LOCUS       DADMKF010000001        88080 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.001, whole genome shotgun sequence.
ACCESSION   DADMKF010000001 DADMKF010000000
VERSION     DADMKF010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 88080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 88080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..88080
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.001"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(<1..903)
                     /gene="nac"
                     /locus_tag="JNC24_000001"
     CDS             complement(<1..903)
                     /gene="nac"
                     /locus_tag="JNC24_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator"
                     /protein_id="HAY9156870.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVATLEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQALSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEIVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQSVDVNAIAKMNLFLPSDYSAIRLRVDEAFSLRRFTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQW"
     gene            1230..1305
                     /locus_tag="JNC24_000002"
     tRNA            1230..1305
                     /locus_tag="JNC24_000002"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1263..1265,aa:Asn,seq:gtt)
     gene            complement(1361..2297)
                     /gene="ldtA"
                     /locus_tag="JNC24_000003"
                     /pseudo
     CDS             complement(1361..2297)
                     /gene="ldtA"
                     /locus_tag="JNC24_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310812.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
     gene            complement(2359..3438)
                     /gene="cobT"
                     /locus_tag="JNC24_000004"
     CDS             complement(2359..3438)
                     /gene="cobT"
                     /locus_tag="JNC24_000004"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HAY9156871.1"
                     /translation="MQTLADLLNTIPAIDPAAMSRAQRHIDGLLKPVGSLGRLEALAI
                     QLAGMPGLNGVPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVYVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRHLAEKLLLDVICYT
                     RELAKNGVTLFGVGDLGMANTTPAAAIVSTITGRAPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGMAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMEMRLGEGSGAALAMPIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(3450..4193)
                     /gene="cobS"
                     /locus_tag="JNC24_000005"
     CDS             complement(3450..4193)
                     /gene="cobS"
                     /locus_tag="JNC24_000005"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HAY9156872.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGVPLAALFSVLVLALMTGGFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLPGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            complement(4190..4735)
                     /gene="cobU"
                     /locus_tag="JNC24_000006"
     CDS             complement(4190..4735)
                     /gene="cobU"
                     /locus_tag="JNC24_000006"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HAY9156873.1"
                     /translation="MMILVTGGARSGKSRHAEALIGDSSQVLYIATSQILDDEMAARI
                     EHHRQGRPAHWHTVERWQHLDELIHADINPHEAVLLECVTTMVTNLLFDYGGDKDPDE
                     WDYQAMEQAINAEIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQ
                     QLAAAANEVWLVVSGIGVKIK"
     gene            complement(4869..5306)
                     /locus_tag="JNC24_000007"
     CDS             complement(4869..5306)
                     /locus_tag="JNC24_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HAY9156874.1"
                     /translation="MKTIKPESIALFCLLSGDVDLAKHLAEILPITCFVGEDWQQDFQ
                     PLDGGPVQSLSDAYSDYSVLLLIAAESEISVPEWLAHSEGTTVILIAEHNARLLTGPA
                     GGAVALIHYLNEICHSRETAVNDAFCRTLTRGSLAIPQCHLLS"
     regulatory      complement(5524..5699)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(5788..6464)
                     /locus_tag="JNC24_000008"
                     /pseudo
     CDS             complement(5788..6464)
                     /locus_tag="JNC24_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460981.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
     gene            complement(6667..7476)
                     /gene="pduF"
                     /locus_tag="JNC24_000009"
     CDS             complement(6667..7476)
                     /gene="pduF"
                     /locus_tag="JNC24_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol diffusion facilitator PduF"
                     /protein_id="HAY9156875.1"
                     /translation="MNDSLKAQCVAEFLGTGLFLFFGIGCLCALKVAGASLGLWEICI
                     IWGLGISLAVYLTAGISGAHLNPAVTIALWLFASFPARKVLPFCLAQLAGAFGGAVLA
                     YSLYSSLFTDFESAHNMVRGSAESLQLASIFSTYPAAAINVWQAALVEVVITSMLMGL
                     IMALTDDGNGVPKGPLAPLLIGILVAVIGASTGPLTGFAMNPARDFGPKIFTWLAGWG
                     EIAMTGGRNIPYFIVPIIAPIIGACVGAAIYRYLIAKNLPVNSVTPKGISE"
     gene            7988..8272
                     /gene="pduA"
                     /locus_tag="JNC24_000010"
     CDS             7988..8272
                     /gene="pduA"
                     /locus_tag="JNC24_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduA"
                     /protein_id="HAY9156876.1"
                     /translation="MQQEALGMVETKGLTAAIEAADAMVKSANVLLVGYEKIGSGLVT
                     VIVRGDVGAVKAATDAGAAAAHNVGEVKAVHVIPRPHTDVEKILPKGINP"
     gene            8269..9081
                     /gene="pduB"
                     /locus_tag="JNC24_000011"
     CDS             8269..9081
                     /gene="pduB"
                     /locus_tag="JNC24_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduB"
                     /protein_id="HAY9156877.1"
                     /translation="MSSNELVEQIMAQVIARVATPEHEVLPKTTHQIRETAMAEKSCT
                     LTEFVGTAIGDTVGLVIANVDSALLDAMKLEKRYRSIGILGARIGAGPHIMAADEAVK
                     ATNTEVISIELPRDTKGGAGHGSLILLGGNDVSDVKRGIEVALKELDRTFGDVYANEA
                     GHLELQYTARASYALEKAFGAPLGRACGVIVGAPAAVGVLMADTALKSANVDVVAYSS
                     PAQDTSYSNEVILIISGDSGAVRQAVISAREIGKTVLSTLGDTPKNDHPSYI"
     gene            9100..10764
                     /gene="pduC"
                     /locus_tag="JNC24_000012"
     CDS             9100..10764
                     /gene="pduC"
                     /locus_tag="JNC24_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol dehydratase large subunit PduC"
                     /protein_id="HAY9156878.1"
                     /translation="MRSKRFEALAKRPVNQDGFVKEWIEEGFIAMESPNDPKPSIKIV
                     NGVVTELDGKPASQFDLIDHFIARYGINLARAEEVIAMDSVKLANMLCDPNVKRSDIV
                     PLTTAMTPAKIVEVVSQMNVVEMMMAMQKMRARRTPSQQAHVTNVKDNPVQIAADAAE
                     GAWRGFDEQETTVAVARYAPFNAIALLVGSQVGRPGVLTQCSLEEATELKLGMLGHTC
                     YAETISVYGTEPVFTNGDDTPWSKGFLASSYASRGLKMRFTSGSGSEVQMGYAEGKSM
                     LYLEARCIYITKAAGVQGLQNGSVSCIGVPSAVPSGIRAVLAENLICSSLDLECASSN
                     DQTFTHSDMRRTARFLMQFLPGTDFISSGFSAVPNYDNMFAGSNEDAEDFDDYNVIQR
                     DLKVDGGLRPVREEDVIAIRNKAARALQAVFAGMGLPPITDEEVEAATYAHGSKDMPE
                     RNIVEDIKFAQEIINKNRNSLEVVKALAQGSFTDVAQDMLNMQKAKLTGDYLHTSAII
                     VDDGQVLSAVNDVNDYAGPATGYRLQGERWEEIKNIPGALDPNEID"
     gene            10774..11442
                     /locus_tag="JNC24_000013"
     CDS             10774..11442
                     /locus_tag="JNC24_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol/glycerol family dehydratase medium
                     subunit"
                     /protein_id="HAY9156879.1"
                     /translation="MEINEKLLRQIIEDVLAEMQPSDKSVSFRAPVSATVPSAPDTGN
                     FLTEIGEAQQGTQQDEVIIAVGPAFGLAQTVNIIGIPHKNILREVIAGIEEEGIKARV
                     IRCFKSSDVAFVAVEGDRLSGSGIAIGIQSKGTTVIHQQGLPPLSNLELFPQAPLLTL
                     DTYRQIGKNAARYAKRESPQPVPTLNDQMARPKYQAKSAILHIKETKYVVTGKKPQEL
                     RVTF"
     gene            11457..11975
                     /gene="pduE"
                     /locus_tag="JNC24_000014"
     CDS             11457..11975
                     /gene="pduE"
                     /locus_tag="JNC24_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol dehydratase small subunit PduE"
                     /protein_id="HAY9156880.1"
                     /translation="MNTDAIESMVRDVLNRMNSLQDAAPVSAVPNASILSAKVTDYPL
                     ANKHPEWVKTATNKTLDDFTLENVLSDNVTALDMRITPETLRIQAAIARDAGRDRLAM
                     NFERAAELTSVPDDRILEIYNALRPYRSTKQELIAIADDLEQRYQAKICAAFVREAAE
                     LYVERKKLKGDD"
     gene            11985..13817
                     /locus_tag="JNC24_000015"
     CDS             11985..13817
                     /locus_tag="JNC24_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diol dehydratase reactivase subunit alpha"
                     /protein_id="HAY9156881.1"
                     /translation="MRYIAGIDIGNSSTEVALATLSATGELSFVSSALEETTGIKGTL
                     RNVHGIQEALAQATKKVGINVSDISLIRINEATPVIGDVAMETITETIITESTMIGHN
                     PKTPGGVGLGVGFTITPQELLTRPADTPYILVVSSAFDFADIATMINASVRAGYQLTG
                     VILQRDDGVLVNNRLEIPLPIVDEVLYIDRIPLGMLAAIEVAVPGKVIETLSNPYGIA
                     TVFALNAEETKNIVPVARALIGNRSAVVVKTPSGDVKARSIPAGNIELLSAGRTTRVD
                     VAAGADAIMKAVGECPKLENVTGEPGTNIGGMLEHVRQTMAELTNKPSNEIFIQDLLA
                     IDTSVPVSVTGGLAGEFSLEQAVGIASMVKSDRLQMAMIASEIKQKLHVDVQVGGAEA
                     EAAIQGALTTPGTTRPLAILDLGAGSTDASIINQSGEIVATHLAGAGDMVTMIIAREL
                     GLNDRYLAEEIKKYPLAKVESLFHLRHEDGSVQFFPTPLSPHVFARVCVVKPDELVPI
                     PGDLTLEKVRAVRRSAKERVFVTNALRALRQVSPAGNIRDIPFVVLVGGSSLDFEVPQ
                     LVTDALAHYRLVAGRGNIRGSEGPRNAVATGLLIAWHKESIHGK"
     gene            13807..14157
                     /locus_tag="JNC24_000016"
     CDS             13807..14157
                     /locus_tag="JNC24_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol dehydratase"
                     /protein_id="HAY9156882.1"
                     /translation="MESKERAPAIVVMEIGDCITTWDEVLLGIEEEGIPFRIQHIPSG
                     EVIDSAWLAARQSPLLVGIACDREELIVHYKNLPASAPLFTLTYQQDNHTRRSIGTNA
                     ARLVKGIPFREFHS"
     gene            14177..14452
                     /gene="pduJ"
                     /locus_tag="JNC24_000017"
     CDS             14177..14452
                     /gene="pduJ"
                     /locus_tag="JNC24_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduJ"
                     /protein_id="HAY9156883.1"
                     /translation="MNNALGLVETKGLVGAIEAADAMVKSANVQLVGYEKIGSGLITV
                     MVRGDVGAVKAAVDAGSAAASAVGEVKSCHVIPRPHSDVEAILPKSA"
     gene            14476..14898
                     /locus_tag="JNC24_000018"
     CDS             14476..14898
                     /locus_tag="JNC24_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003844245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HAY9156884.1"
                     /translation="MKQSLGLLEASGLALAISCADAMAKAASITLVGLEKTNGSGWTL
                     IKITGDVASVNAAIATGASFAGQRNGLVAHKVIARPGDGILPHTTEALTVTVETSKTP
                     ATTVTSEIISCNLCLDPACQRQKGEPRSLCLYANKRGE"
     gene            14901..15533
                     /locus_tag="JNC24_000019"
     CDS             14901..15533
                     /locus_tag="JNC24_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HAY9156885.1"
                     /translation="MDKQTLQETVSQILDEMRERPIPLGVSSRHIHLSSADYLRLFPE
                     QPISEKKPLMQPGQYAAEQVVTLEGPKGKLHNVRLLGPLRNASQVEISRTDARTLGIT
                     APLRMSGDLSGTPGIRLISPFAELEITSGVIVAQRHIHMSPLDALIYGVTHGDKVAVA
                     IEGDERRLIFENVAVRVSPDMRLEMHIDTDEANAAGADSPDVFATLVSVP"
     gene            15530..16021
                     /gene="pduM"
                     /locus_tag="JNC24_000020"
     CDS             15530..16021
                     /gene="pduM"
                     /locus_tag="JNC24_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcompartment protein PduM"
                     /protein_id="HAY9156886.1"
                     /translation="MNNMLMQRIVDEVVFRLKQRAGKTLVLTVFQLRDASVQESVHQY
                     ASLQIRYVDLPLLRQLAENETSDRASIQIHEALAWGMHIQLSLQRHFLNAIELKTLAR
                     LPLSWCDEQGQPIYLHHNRLLSYADIAQLSSGILVLQRKCCVTALAREAAITRNIQLI
                     RQE"
     gene            16025..16300
                     /gene="pduN"
                     /locus_tag="JNC24_000021"
     CDS             16025..16300
                     /gene="pduN"
                     /locus_tag="JNC24_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduN"
                     /protein_id="HAY9156887.1"
                     /translation="MHLARVTGAVVSTQKSPSLIGKKLLLVRRVSADGELPLTPLTGD
                     EVAVDSVGAGVGELVLLSSGSSARHVFSGPNEAIDLAVVGIVDTLSR"
     gene            16312..17319
                     /gene="pduO"
                     /locus_tag="JNC24_000022"
     CDS             16312..17319
                     /gene="pduO"
                     /locus_tag="JNC24_000022"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-domain cob(I)yrinic acid a,c-diamide
                     adenosyltransferase PduO"
                     /protein_id="HAY9156888.1"
                     /translation="MAIYTRTGDSGSTSLFTGQRVSKTHLRVETYGTLDELNATLSLC
                     YCATAIESHRILLEAIQQQIFWFSAELASESEQPSAQQRYIGTEEIAALENAIDSAMN
                     AVPPVHSFILPGRCEAASRMHFARTVARRAERRLVELTTETTVRNVLLHYINRLSDCL
                     YALARVEDNVAHQNLMIQEITKRYHEANHTPALKERTMPLTFQDLHQLIRSAAMRADE
                     LHIPVVISIVDANGTESVTWRMPDALLVSSELAPKKAWTAVAMKTATHKLADTVQPDA
                     PLYGLESHMQGKVVTFGGGFPLWRDGKLLGGLGISGGSVEQDMDIAQSAMAAINVGVN
                     Q"
     gene            17316..18701
                     /gene="pduP"
                     /locus_tag="JNC24_000023"
                     /pseudo
     CDS             17316..18701
                     /gene="pduP"
                     /locus_tag="JNC24_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460996.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating propionaldehyde dehydrogenase
                     PduP"
     gene            18712..19824
                     /locus_tag="JNC24_000024"
     CDS             18712..19824
                     /locus_tag="JNC24_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001091448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HAY9156889.1"
                     /translation="MNTFSLQTRLYSGPGSLKVLSRFSHRHIWIICDAFLARSPLIDT
                     LHQALPDSNRLSIFSEITPAPTIQTVVKGIAQMQTLRPDVVIGFGGGSALDAAKAIVW
                     FGRQCGIEIETCVAIPTTSGIGSEVTSACVISDPEKGIKYPLFDNALYPDIAILDPSL
                     VVSVPPAITANTGMDVLTHALEAYVSPRASDFTDALVEKAVQIVFQYLPTAVKKGDCL
                     ATRGKMHNASTLAGMAFSQAGLGLNHAIAHQLGGQFHLPHGLANALLLTAVIRFNAGD
                     PRTAKRYARLAKTCHLCPDNANDTASLNALIQHIEQLNTTCTLPTLANALKEKKAEWS
                     IRIPDMVQAALADATLRTNPRAADASAIAELLKGLL"
     gene            19821..21166
                     /locus_tag="JNC24_000025"
                     /pseudo
     CDS             19821..21166
                     /locus_tag="JNC24_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585845.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex protein RnfC"
     gene            21169..21723
                     /gene="pduT"
                     /locus_tag="JNC24_000026"
     CDS             21169..21723
                     /gene="pduT"
                     /locus_tag="JNC24_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduT"
                     /protein_id="HAY9156890.1"
                     /translation="MSKAVGILELSSIAKGMETGDAMLKSANVELLVSKTLCPGKFLL
                     MLGGDVGAVQQAINSGASLAGEMLVDSLVLPNIHPSLLSAISGLNHVEQHRAVGVVET
                     WSVAACIDAADCAVKAANVILVRVHMAFGIGGKCYMVVAGDISDVNNAVSAASVRAGE
                     KGLLVYRSVIARPHEAMWRQMVEG"
     gene            21723..22073
                     /gene="pduU"
                     /locus_tag="JNC24_000027"
     CDS             21723..22073
                     /gene="pduU"
                     /locus_tag="JNC24_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000441103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduU"
                     /protein_id="HAY9156891.1"
                     /translation="MTTQHTTERMIQEYVPGKQVTLAHVIANPGKDLFKKLGLPDTIS
                     AIGILTITPSEASIIACDIATKSGAVEIGFLDRFTGAVVLTGDVSAIEYALSQVTRTL
                     GEMMRFTACPITRT"
     gene            22078..22521
                     /gene="eutP"
                     /locus_tag="JNC24_000028"
     CDS             22078..22521
                     /gene="eutP"
                     /locus_tag="JNC24_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
                     /protein_id="HAY9156892.1"
                     /translation="MQRMMLIGPSQCGKTSLTQCLRGENIHYQKTQAIVWSPTTIDTP
                     GEYLENRSLYSALLVSACEADIIALVLNADAPWSPFSPGFTSSMNRPVIGVVTKADLA
                     NMEQISLVKCWLREAGAHNVLVTSAVNNHGVAELLALLHTEEGCR"
     gene            complement(22622..22951)
                     /locus_tag="JNC24_000029"
     CDS             complement(22622..22951)
                     /locus_tag="JNC24_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HAY9156893.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(23123..24181)
                     /locus_tag="JNC24_000030"
     CDS             complement(23123..24181)
                     /locus_tag="JNC24_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAY9156894.1"
                     /translation="MRADKSLSPFEIRVYRHYRIVHGTRVALAFLLTFLIIRLFTIPE
                     STWPLVTMVVIMGPISFWGNVVPRAFERIGGTVLGSILGLIALQLELISLPLMLVWCA
                     AAMFLCGWLALGKKPYQGLLIGVTLAIVVGSPTGEIDTALWRSGDVILGSLLAMLFTG
                     IWPQRAFIHWRIQLAKSLTEYNRVYQSAFSPNLLERPRLESHLQKLLTDAVKMRGLIA
                     PASKETRIPKSIYEGIQTINRNLVCMLELQINAYWATRPSHFVLLNAQKLRDTQHMMQ
                     QILLSFVHALYEGNPQPVFANTEKLNDAVEELRQLLNNHHDLKVVETPIYGYVWLNME
                     TAHQLELLSNLICRALRK"
     gene            complement(24379..24852)
                     /gene="sbmC"
                     /locus_tag="JNC24_000031"
     CDS             complement(24379..24852)
                     /gene="sbmC"
                     /locus_tag="JNC24_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor SbmC"
                     /protein_id="HAY9156895.1"
                     /translation="MNYEITQEEKRTVAGFHLVGPWEQTVKKGFEQLMMWVDNKNIVP
                     KEWVAVYYDNPDETPAEKLRCDTVVTVPNNFTLPENSEGVILTEISGGQYAVAVARVV
                     GDDFAKPWYQFFNSLLQDSAYEMLPKPCFEVYLNNGAEDGYWDIEMYVAVQPKHH"
     gene            complement(24971..26137)
                     /gene="dacD"
                     /locus_tag="JNC24_000032"
     CDS             complement(24971..26137)
                     /gene="dacD"
                     /locus_tag="JNC24_000032"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase DacD"
                     /protein_id="HAY9156896.1"
                     /translation="MKRRLIIAASLFVFNLSSGFAAENIPFSPQPPEIHAGSWVLMDY
                     TTGQILTAGNEHQQRNPASLTKLMTGYVVDRAIDSHRITPDDIVTVGRDAWAKDNPVF
                     VGSSLMFLKEGDRVSVRDLSRGLIVDSGNDACVALADYIAGGQRQFVEMMNNYAEKLH
                     LKDTHFETVHGLDAPGQHSSAYDLAVFSRAIIHGEPEFYHMYSEKSLTWNGITQQNRN
                     GLLWDKTMNVDGLKTGHTSGAGFNLIASAVDGQRRLIAVVMGADSAKGREEEARKLLR
                     WGQQNFTTVQILHRGKKVGTERIWYGDKENIDLGTEQEFWMVLPKAEIPHIKAKYTLD
                     GKELTAPISAHQRVGEIELYDRDKQVAHWPLVTLESVGEGGMFSRLSDYFHHKA"
     gene            26346..27773
                     /gene="sbcB"
                     /locus_tag="JNC24_000033"
     CDS             26346..27773
                     /gene="sbcB"
                     /locus_tag="JNC24_000033"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HAY9156897.1"
                     /translation="MMNDGKQQSTFLFHDYETFGTHPALDRPAQFAAIRTDNEFNVIG
                     EPEVFYCKPADDYLPQPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGY
                     NNVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDG
                     LPSFRLEHLTKANGIEHSNAHDAMADVCATIAMAKLVKTRQPRLFDYLFTHRNKHKLM
                     ALIDVPQMKPLVHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDS
                     DTLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNL
                     KILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLP
                     ALDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYADELQML
                     AQQYADNKEKVALLKALWQYAEEIV"
     gene            complement(27816..28043)
                     /locus_tag="JNC24_000034"
     CDS             complement(27816..28043)
                     /locus_tag="JNC24_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HAY9156898.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(28057..29115)
                     /gene="yeeE"
                     /locus_tag="JNC24_000035"
     CDS             complement(28057..29115)
                     /gene="yeeE"
                     /locus_tag="JNC24_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transport protein YeeE"
                     /protein_id="HAY9156899.1"
                     /translation="MFSMILSGLICGALLGFVMQRGRFCLTGGFRDMYIAKNNRMFYA
                     LLIAISVQSVGVFALIQAGLLTYEAEAFPWLGTVIGGYIFGLGIVLAGGCATGTWYRA
                     GEGLIGSWIALFTYMVMSAVMRSPHASGLNQTLQHYSTEHNSIAETFNLSVWPLVAVL
                     LVITLWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLS
                     EATGRMFGLGITSPTANILQFLVAGDVKYINWGVFLVLGIFVGSFIAAKASREFRVRA
                     ADAQTTLRSGLGGVLMGFGASIAGGCFIGNGLVMTAMMTWQGWIGLVFMILGVWTASW
                     LVYVRPQRKARLATAAAN"
     gene            complement(29294..30652)
                     /gene="plaP"
                     /locus_tag="JNC24_000036"
     CDS             complement(29294..30652)
                     /gene="plaP"
                     /locus_tag="JNC24_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HAY9156900.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(30642..30704)
                     /gene="yoeI"
                     /locus_tag="JNC24_000037"
     CDS             complement(30642..30704)
                     /gene="yoeI"
                     /locus_tag="JNC24_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HAY9156901.1"
                     /translation="MGQFFAYATVITVKENDHVA"
     gene            complement(30919..31848)
                     /locus_tag="JNC24_000038"
     CDS             complement(30919..31848)
                     /locus_tag="JNC24_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY9156902.1"
                     /translation="MKQLLDVLMILDALEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLAQEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLVQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAI"
     gene            complement(31894..32718)
                     /locus_tag="JNC24_000039"
     CDS             complement(31894..32718)
                     /locus_tag="JNC24_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAY9156903.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLEPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(32801..33055)
                     /gene="yoeB"
                     /locus_tag="JNC24_000040"
     CDS             complement(32801..33055)
                     /gene="yoeB"
                     /locus_tag="JNC24_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HAY9156904.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
     gene            complement(33052..33303)
                     /gene="yefM"
                     /locus_tag="JNC24_000041"
     CDS             complement(33052..33303)
                     /gene="yefM"
                     /locus_tag="JNC24_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HAY9156905.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDIIE"
     gene            33586..33636
                     /gene="hisL"
                     /locus_tag="JNC24_000042"
     CDS             33586..33636
                     /gene="hisL"
                     /locus_tag="JNC24_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HAY9156906.1"
                     /translation="MTRVQFKHHHHHHHPD"
     gene            33782..34681
                     /gene="hisG"
                     /locus_tag="JNC24_000043"
     CDS             33782..34681
                     /gene="hisG"
                     /locus_tag="JNC24_000043"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HAY9156907.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            34687..35991
                     /gene="hisD"
                     /locus_tag="JNC24_000044"
     CDS             34687..35991
                     /gene="hisD"
                     /locus_tag="JNC24_000044"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HAY9156908.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     ARGDDALREYSAKFDKTTVNALKVSAEEIAAASERLSDELKQAMAVAVKNIETFHTAQ
                     KLPPVDVETQPGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCGVKDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MAHQVAEAVERQLAELPRAETARQALNASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARDLVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNALKEQA"
     gene            35988..37058
                     /gene="hisC"
                     /locus_tag="JNC24_000045"
     CDS             35988..37058
                     /gene="hisC"
                     /locus_tag="JNC24_000045"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HAY9156909.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FQLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTQDNWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLTEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIAPYPLSTPVADIAAQALSPQGIVAMRERVTQIIAER
                     EYLIAALKEIPCVEQVFDSATNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            37058..38125
                     /locus_tag="JNC24_000046"
     CDS             37058..38125
                     /locus_tag="JNC24_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase"
                     /protein_id="HAY9156910.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVERYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDREILNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKMALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            38125..38715
                     /gene="hisH"
                     /locus_tag="JNC24_000047"
     CDS             38125..38715
                     /gene="hisH"
                     /locus_tag="JNC24_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HAY9156911.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDVVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            38715..39452
                     /gene="hisA"
                     /locus_tag="JNC24_000048"
     CDS             38715..39452
                     /gene="hisA"
                     /locus_tag="JNC24_000048"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HAY9156912.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPERVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            39434..40210
                     /gene="hisF"
                     /locus_tag="JNC24_000049"
     CDS             39434..40210
                     /gene="hisF"
                     /locus_tag="JNC24_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015853929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HAY9156913.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDGETGKYHVNQYTGDESRTRVTQWETL
                     DWVQEVQKRGAGEIVLNMMNQDGVRNGYDLEQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            40204..40815
                     /locus_tag="JNC24_000050"
     CDS             40204..40815
                     /locus_tag="JNC24_000050"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HAY9156914.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVNIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGDTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ"
     gene            complement(40912..41889)
                     /gene="wzzB"
                     /locus_tag="JNC24_000051"
     CDS             complement(40912..41889)
                     /gene="wzzB"
                     /locus_tag="JNC24_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HAY9156915.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIITQPDVGQIAGYNNAMNVIYGQAAPKVSDLQETLIGRFSSA
                     FSALAETLDNQEEPEKLTIEPSVKNQQLPLTVSYVGQTAEGAQMKLAQYIQQVDDKVN
                     QELEKDLKDNIALGRKNLQDSLRTQEVVAQEQKDLRIRQIQEALQYANQAQVAKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIENLKVDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(42035..43201)
                     /gene="ugd"
                     /locus_tag="JNC24_000052"
     CDS             complement(42035..43201)
                     /gene="ugd"
                     /locus_tag="JNC24_000052"
                     /EC_number="1.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HAY9156916.1"
                     /translation="MKITISGTGYVGLSNGLLIAQNHEVVALDILPSRVAMLNDRISP
                     IVDKEIQQFLQSDKIHFNATLDKNEAYRDADYVIIATPTDYDPKTNYFNTSSVESVIK
                     DVVEINPYAVMVIKSTVPVGFTEAMHKKYRTENIIFSPEFLREGKALYDNLHPSRIVI
                     GERSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNTRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADAILSRKPQVVGIYRLIMKSGSDNFRASSIQGIMKRIKAKGVEVII
                     YEPVMKEDSFFNSRLERDLAIFKQQADVIISNRMAEELKDVADKVYTRDLFGSD"
     gene            complement(43451..44857)
                     /gene="gndA"
                     /locus_tag="JNC24_000053"
     CDS             complement(43451..44857)
                     /gene="gndA"
                     /locus_tag="JNC24_000053"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HAY9156917.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQSAGDKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            complement(45021..46391)
                     /gene="cpsG"
                     /locus_tag="JNC24_000054"
     CDS             complement(45021..46391)
                     /gene="cpsG"
                     /locus_tag="JNC24_000054"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707943.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase CpsG"
                     /protein_id="HAY9156918.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALARGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAQPFEAINR
                     VEQHFSREALAVDRTDGISMTFADWRFNLRTSNTEPVVRLNVESRGDVPLMEEKTKLI
                     LELLNK"
     gene            complement(46496..47932)
                     /gene="cpsB"
                     /locus_tag="JNC24_000055"
     CDS             complement(46496..47932)
                     /gene="cpsB"
                     /locus_tag="JNC24_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HAY9156919.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPESDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSAGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMERTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGAMHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            complement(47935..49158)
                     /gene="wcaI"
                     /locus_tag="JNC24_000056"
     CDS             complement(47935..49158)
                     /gene="wcaI"
                     /locus_tag="JNC24_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HAY9156920.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGSKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAAENVIFFPNWSEIARFQHVADADV
                     DALRNQLDLPDNKKIILYSGNIGEKQGLENVIEAADRLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            complement(49155..49634)
                     /locus_tag="JNC24_000057"
     CDS             complement(49155..49634)
                     /locus_tag="JNC24_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HAY9156921.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELILPDEQHDDYRWLTPDALLASNDVHANSRAYFLAEKRAGVPGL"
     gene            complement(49637..50602)
                     /gene="fcl"
                     /locus_tag="JNC24_000058"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             complement(49637..50602)
                     /gene="fcl"
                     /locus_tag="JNC24_000058"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HAY9156922.1"
                     /translation="MSKQRIFIAGHRGMVGSAIRRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     LFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRG"
     gene            complement(50605..51726)
                     /gene="gmd"
                     /locus_tag="JNC24_000059"
     CDS             complement(50605..51726)
                     /gene="gmd"
                     /locus_tag="JNC24_000059"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HAY9156923.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            complement(51752..52300)
                     /gene="wcaF"
                     /locus_tag="JNC24_000060"
     CDS             complement(51752..52300)
                     /gene="wcaF"
                     /locus_tag="JNC24_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HAY9156924.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETE"
     gene            complement(52316..53062)
                     /gene="wcaE"
                     /locus_tag="JNC24_000061"
     CDS             complement(52316..53062)
                     /gene="wcaE"
                     /locus_tag="JNC24_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HAY9156925.1"
                     /translation="MLLSIITVAFRNLEGIVKTHASLAHLAQAEDISFEWIVVDGGSN
                     DGTREYLENLNGIYNLRFVSEPDNGIYDAMNKGITMAQGKFALFLNSGDIFHQDAANF
                     VRKLKMQKDNMMITGDALLDFGDGHKIKRSAKPGWYIYHSLPASHQAIFFPVSGLKKW
                     RYDLEYKVSSDYALAAKMYKAGYAFKKLNGLVSEFSMGGVSTTNNMELCADAKKVQRQ
                     ILHVPGFWAELSWHLRQRTTSKTKALYNKV"
     gene            complement(53073..54290)
                     /gene="wcaD"
                     /locus_tag="JNC24_000062"
     CDS             complement(53073..54290)
                     /gene="wcaD"
                     /locus_tag="JNC24_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative colanic acid polymerase WcaD"
                     /protein_id="HAY9156926.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISVKKLMIALGIGAGLTAFNYLFGQSLDASKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVIGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDAMILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFRVGVVIAFPYISTRLGDLGTEGS
                     SSYYRIVGPLVMVGYSLTHIDGVVRFGSLYEYVASFGIFNGADVGKTIDNGLYLLIIY
                     FSWFAVFLSLWYMGKVIKMMINAFGDNRNFRVQLYLFTPVSLFFTGSIFSPEYAFLIV
                     CPFILRKALNITR"
     gene            complement(54265..55482)
                     /gene="wcaC"
                     /locus_tag="JNC24_000063"
     CDS             complement(54265..55482)
                     /gene="wcaC"
                     /locus_tag="JNC24_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HAY9156927.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLKSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVDEEDVLQLVQLSKPEIAQAIFGTTLAEFSQRSRATYSGQ
                     QMLEEYVNFYQNL"
     gene            complement(55479..55967)
                     /gene="wcaB"
                     /locus_tag="JNC24_000064"
     CDS             complement(55479..55967)
                     /gene="wcaB"
                     /locus_tag="JNC24_000064"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HAY9156928.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            complement(55970..56809)
                     /gene="wcaA"
                     /locus_tag="JNC24_000065"
     CDS             complement(55970..56809)
                     /gene="wcaA"
                     /locus_tag="JNC24_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HAY9156929.1"
                     /translation="MKENPLISIYMPTWNRQQLAIRAIKSVLRQDYSNWEMIIVDDCS
                     TSWEQLQQYVTALNDPRITYIHNDINSGACAVRNQAIMLAQGEYITGIDDDDEWTPNR
                     LSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
                     FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSP
                     KKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGI
                     RGR"
     gene            complement(56902..59064)
                     /gene="wzc"
                     /locus_tag="JNC24_000066"
     CDS             complement(56902..59064)
                     /gene="wzc"
                     /locus_tag="JNC24_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HAY9156930.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITAVFALC
                     AVVYTFFATPIYSADALVQIEQNSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWERLMGRQNETVKVTTFNRPKEMAGQVFTLNVLDNK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHARPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEEEKAKLNGRVTAMPKTQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIVDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGIKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVSWASKNYDLVLIDTPPILAVTDAAIVGRHAGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            complement(59067..59510)
                     /gene="wzb"
                     /locus_tag="JNC24_000067"
     CDS             complement(59067..59510)
                     /gene="wzb"
                     /locus_tag="JNC24_000067"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HAY9156931.1"
                     /translation="MFNNILVVCVGNICRSPTAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMEKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            complement(59516..60655)
                     /locus_tag="JNC24_000068"
     CDS             complement(59516..60655)
                     /locus_tag="JNC24_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HAY9156932.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALGNAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            61314..62897
                     /locus_tag="JNC24_000069"
     CDS             61314..62897
                     /locus_tag="JNC24_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HAY9156933.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPRQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEEEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIVGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLHKDGELEYEV"
     gene            complement(63171..65024)
                     /gene="asmA"
                     /locus_tag="JNC24_000070"
     CDS             complement(63171..65024)
                     /gene="asmA"
                     /locus_tag="JNC24_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HAY9156934.1"
                     /translation="MRRFLTTLMILLVVLVAGLSVLVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQAQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGQVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(65046..65627)
                     /gene="dcd"
                     /locus_tag="JNC24_000071"
     CDS             complement(65046..65627)
                     /gene="dcd"
                     /locus_tag="JNC24_000071"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HAY9156935.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(65719..66360)
                     /gene="udk"
                     /locus_tag="JNC24_000072"
     CDS             complement(65719..66360)
                     /gene="udk"
                     /locus_tag="JNC24_000072"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HAY9156936.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            66678..69995
                     /locus_tag="JNC24_000073"
     CDS             66678..69995
                     /locus_tag="JNC24_000073"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HAY9156937.1"
                     /translation="MSKQSQHVLIALPHPLLHLVSLGLVSFIFTLFSLELSQFGTQLA
                     PLWFPTSIMMVAFYRHAGRMWPGIALSCSLGNIAASILLFSTSSLNMTWTTINIVEAV
                     VGAVLLRKLLPWYNPLQNLADWLRLALGSAIVPPLLGGVLVVLLTPGDDPLRAFLIWV
                     LSESIGALALVPLGLLFKPHYLLRHRNPRLLFESLLTLAITLTLSWLSMLYLPWPFTF
                     IIVLLMWSAVRLPRMEAFLIFLTTVMMVSLMMAADPSLLATPRTYLMSHMPWLPFLLI
                     LLPANIMTMVMYAFRAERKHISESETRFRNAMEYSAIGMALVGTEGQWLQTNKALCQF
                     LGYSQEELRGLTFQQLTWPEDLNKDLQQVEKLISGEINTYSMEKRYYNRNGDVVWALL
                     AVSLVRHTDGTPLYFIAQIEDINELKRTEQVNQQLMERITLANEAGGIGIWERELKPN
                     VFSWDKRMFELYEIPPHIKPNWQVWYECVLPEDRQHAEKVIRDSLQSRSPFKLEFRIT
                     VKDGIRHIRALANRVLNKEGEVERLLGINMDMTEVKQLNEALFQEKERLHITLDSIGE
                     AVVCIDMAMKITFMNPVAEKMSGWTQEEALGVPLLTVLHITFGDNGPLMENIYSADTS
                     RSAIEQDVVLHCRSGGSYDVHYSITPLSTLDGSNIGSVLVIQDVTESRKMLRQLSYSA
                     SHDALTHLANRASFEKQLRILLQTVNSTHQRHALVFIDLDRFKAVNDSAGHAAGDALL
                     RELASLMLSMLRSSDVLARLGGDEFGLLLPDCNVESARFIATRIISAVNDYHFIWEGR
                     VHRVGASAGITLIDDNNHQAAEVMSQADIACYASKNGGRGRVTVYEPQQAAAHSERAA
                     MSLDEQWRMIKENQLMMIAHGVASPRIPEARNLWLISLKLWSCEGEIIDEQTFRRSFS
                     DPALSHALDRRVFHEFFQQAAKAVASKGISIALPLSVAGLSSATLVNDLLEQLENSPL
                     PPRLLHLIIPAEAILDHAESVQKLQLAGCRIVLSQVGRDLQIFNSLKANMADYLLLDG
                     ELCANVQGNLMDEMLITIIQGHAQRLGMKTIAGPVVLPLVMDTLSGIGVDLIYGDVIA
                     DAQPLDLLVNSSYFAIN"
     gene            complement(70105..70953)
                     /gene="alkA"
                     /locus_tag="JNC24_000074"
     CDS             complement(70105..70953)
                     /gene="alkA"
                     /locus_tag="JNC24_000074"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HAY9156938.1"
                     /translation="MYTLNWQPPYDWSWMLGFLAARAVSGVETVADNYYARSLAVGEY
                     RGVVTAIPDIARHTLHINLSAGLEPVAAECLAKMSRLLDLQCNPQIVNGALGKLGAGR
                     PGLRLPGCIDAFEQGVRAILGQLVSVAMAAKLTAKVVQLYGERLDDFPEYICFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLANAALEGSLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKRRFPGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDEA"
     gene            71087..72439
                     /gene="yegD"
                     /locus_tag="JNC24_000075"
     CDS             71087..72439
                     /gene="yegD"
                     /locus_tag="JNC24_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HAY9156939.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPQLLKMENDSTLLPSMLCAPTRE
                     SVSEWLYRHHDVPADDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQQVALFEDLVCAMMLHIRQQAQAQLPEAITQAVIGRPINF
                     QGLGGDEANAQAQEILERAAKRAGFRDVVFQYEPIAAGLDYEATLQEEKRVLVVDIGG
                     GTTDCSLLLMGPQWRSRLDREASLLGHSGCRIGGNDLDIALAFKNLMPLLGMGGETEK
                     GIALPILPWWNAVAINDVPAQSDFYSSANGRLLNDLVRDAREPEKVALLQKVWRQRLS
                     YRLVRSAEECKIALSSVAETRASLPFISNELATLISQRGLESALSQPLARILEQVQLA
                     LDNAQEKPDVIYLTGGSARSPQIKKALAEQLPGIPIAGGDDFGSVTAGLARWAEVVFR
                     "
     gene            complement(72452..74392)
                     /gene="yegI"
                     /locus_tag="JNC24_000076"
     CDS             complement(72452..74392)
                     /gene="yegI"
                     /locus_tag="JNC24_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
                     /protein_id="HAY9156940.1"
                     /translation="MKPTLYTATGECVTPGRELGKGGEGAVYDINEFVDSVAKIYHTP
                     PPALKQDKLAFMAATADAQLLNYVAWPQATLHGGRGGKVIGFMMPKVSGKEPIHMIYS
                     PAHRRQRYPHCAWDFLLYVARNIASSFATVHEHGHVVGDVNQNSFMVGRDSKVVLIDS
                     DSFQINANGTLHLCEVGVSHFTPPELQTLSSFVGFERTENHDNFGLALLIFHVLFGGR
                     HPYSGVPLISDAGNALETDITHFRYAYASDNQRRGLKPPPRSIPLSMLPSDVEAMFQQ
                     AFTESGVATGRPTAKAWVSALDSLRQQLKKCTVSAMHVYPGHLADCPWCALDNQGVIY
                     FIDLGEEVITTGGDFVLAKVWAMVMASVAPPALQLPLPDHFQPTGRPLPLGLLRREYI
                     ILLEIALSALSLLLCGLQAEPRYIILVPVLAAIWIIGSLTSKAYKAEVQQRREAFNRA
                     KMDYDHLVRQIQQVGGLEGFIAKRTMLEKMKDEILGLPEEEKRALAALHDTARERQKQ
                     KFLEGFFIDVASIPGVGPARKAALRSFGIETAADVTRRGVKQVKGFGDHLTQAVIDWK
                     ANCERRFVFRPNEAITPADRQAVMAKMTAKRHRLESALTVGATELQRFRLHAPARTMP
                     LMEPLRQAAEKLAQAQADLSRC"
     gene            complement(74389..75150)
                     /gene="pphC"
                     /locus_tag="JNC24_000077"
     CDS             complement(74389..75150)
                     /gene="pphC"
                     /locus_tag="JNC24_000077"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase PphC"
                     /protein_id="HAY9156941.1"
                     /translation="MSWRLVYASAVGTSHISADLPCQDACQMQVVWLNDQQPLLVMFL
                     ADGAGSVSQGGEGATLAVNEAMAFMVQKVQDGELGLNDVLATNMVLTIRQRLFAEAEA
                     KALAVRDFACTFLGLISSPDGTLIMQIGDGGVVVDLGHGLQLPLTPMVGEYANMTHFI
                     TDEDAVSRLETFTSTGRAHKVAAFTDGIQRLALNMLDNSPHVPFFTPFFNGLASATQE
                     QLDLLPELLKQFLSSPAVNERTDDDKTLALALWLP"
     gene            complement(75147..75806)
                     /locus_tag="JNC24_000078"
     CDS             complement(75147..75806)
                     /locus_tag="JNC24_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAY9156942.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMSGRPINELNAGL
                     VTFRDELLADPLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPTDEWQAAANKVFQGEEDKKFAFFSIG
                     VQGADMKTLAQISVRQPLPLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
     gene            complement(76027..76086)
                     /gene="ibsA"
                     /locus_tag="JNC24_000079"
     CDS             complement(76027..76086)
                     /gene="ibsA"
                     /locus_tag="JNC24_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsA"
                     /protein_id="HAY9156943.1"
                     /translation="MMKHVIILVILLVISFQAY"
     gene            complement(76359..76415)
                     /locus_tag="JNC24_000080"
     CDS             complement(76359..76415)
                     /locus_tag="JNC24_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HAY9156944.1"
                     /translation="MMKLLIIVVLLVISFPAY"
     gene            complement(76686..76742)
                     /locus_tag="JNC24_000081"
     CDS             complement(76686..76742)
                     /locus_tag="JNC24_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HAY9156945.1"
                     /translation="MMKLLIIVVLLVISWPAY"
     gene            77021..78262
                     /gene="mdtA"
                     /locus_tag="JNC24_000082"
     CDS             77021..78262
                     /gene="mdtA"
                     /locus_tag="JNC24_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HAY9156946.1"
                     /translation="MKGSYKSRWVIVVVIAAIAAFWFWQGRNDSQSAAPGATKQAQQS
                     PAGGRRGMRSGPLAPVQAATAVEQAVPRYLTGLGTIIAANTVTVRSRVDGQLMALHFQ
                     EGQQVKAGDLLAEIDPSQFKVALAQTQGQLAKDKATLANARRDLARYQQLAKTNLVSR
                     QELDAQQALVSETEGTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQISSG
                     DTTGIVVITQTHPIDLVFTLPESDIATVVQAQKAGKTLVVEAWDRTNSKKLSEGTLLS
                     LDNQIDATTGTIKVKARFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNEGH
                     FVWVLNSENKVSKHLVTPGIQDSQKVVIRAGISAGDRVVTDGIDRLTEGAKVEVVEAQ
                     SATTPEEKATSREYAKKGARS"
     gene            78262..81384
                     /gene="mdtB"
                     /locus_tag="JNC24_000083"
     CDS             78262..81384
                     /gene="mdtB"
                     /locus_tag="JNC24_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HAY9156947.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVATTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPGASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLDVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSETVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVDDTQFELMMAIALVVMIIYLFLRNIPATIIPGVAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLTFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKMFDRIIAAYGRGLAKVLNHPWLTLSVALSTLLLSVLLWVFIPKGFFPVQD
                     NGIIQGTLQAPQSSSFTNMAQRQRQVADVILQDPAVQSLTSFVGVDGTNPSLNSARLQ
                     INLKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRTQYQFTLQAT
                     SLDALSTWVPQLMEKLQQLPQLSDVSSDWQDQGLVAYVNVDRDSASRLGISMADVDNA
                     LYNAFGQRLISTIYTQANQYRVVLEHNTEITPGLAALDTIRLTSSDGGVVPLSSIAKV
                     EQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQEIMDTEKTLNLPVDITTQFQGST
                     LAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGALLALMIAGS
                     ELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPRDAIYQACLLRFRPILMTT
                     LAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLLFDRLALWTK
                     SRFARHEEEA"
     gene            81385..84462
                     /gene="mdtC"
                     /locus_tag="JNC24_000084"
     CDS             81385..84462
                     /gene="mdtC"
                     /locus_tag="JNC24_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HAY9156948.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLNPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVSVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNIWLYISIPKTFFPEQDTGVLMGGIQA
                     DQSISFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQ
                     QIIDRLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRK
                     KLATLPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIY
                     QPMNQYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGL
                     SAASTISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILI
                     IAAIATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLI
                     GIVKKNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSG
                     GDGSELRHLLGITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            84463..85878
                     /locus_tag="JNC24_000085"
     CDS             84463..85878
                     /locus_tag="JNC24_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY9156949.1"
                     /translation="MTELPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLLLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAIAGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLITLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHVSVDSGTTQTVFMYTWLSMAFIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            85875..87278
                     /gene="baeS"
                     /locus_tag="JNC24_000086"
     CDS             85875..87278
                     /gene="baeS"
                     /locus_tag="JNC24_000086"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HAY9156950.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDEQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            87275..87997
                     /gene="baeR"
                     /locus_tag="JNC24_000087"
     CDS             87275..87997
                     /gene="baeR"
                     /locus_tag="JNC24_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HAY9156951.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLPYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDIPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
BASE COUNT        21561 a        23315 c        22149 g        21055 t
ORIGIN      
        1 ccactgcctt ttttccatca ctggggaact aatcactgac attaacaact ctttcactgc
       61 ctgtgcctgt ggcgataagt tcgctctggc gggtaaattt aatgacaaag agagactcat
      121 ggaaggagtg gtaatgcgtg acatccaccc atttactgcg ccacataacg aacgcgcggc
      181 cgattcgggt aatactgcaa cgcccatgcc gctggcaatc gctgcggtaa gcgtggcaat
      241 agactcaatt tcaccaatga cttttgccgt gaatcgccgt aaggaaaaag cctcatcaac
      301 acgaagtcta atagcactgt aatcactggg gagaaagagg ttcattttcg caatagcatt
      361 cacatcaacg ctttgccccg ggcaatcttg agttcctacc agaaaaagat cttctttcag
      421 caaagcctga ctggatacac cagccacagg ggaatgctca taaatcaccg ccatatcgag
      481 ttggtgattt atcaattttt cgttaagcac tgcaccacta ttttcatgaa gatagataac
      541 gatctccgga aattcagcgc gaaccgcctg taataagggc atggtgatgg atgacgcagc
      601 ggttcctggt gcaaagccaa tcgagacttg ccccgataat gcctgaccaa cgttatgcac
      661 cgccagttgg gcctgttcac actgacgtaa aatggcccgc gcatgggtat agagaatttt
      721 tccggcgtct gttggcgtaa cgccccgctt tgtacggatc aaaagttgtt gatttaactc
      781 accttccagt gtggcaacct gctggctgag cgctggttgt gcgatatgca atacttcagc
      841 agcctgggtc aggctaccaa tatctacaat ttttacgaag tatttcaggc gtctgaagtt
      901 catgttgcct ccggttttta agaatcggcc caagtgccgc cattacttac aaccagattg
      961 caagatgctt gccagtttta ttttggcgtt gatgtacaag ctaaccaact gtcaaataag
     1021 agattatgat agattcgtca tttgctcctt taatcagctg tcgcgttccc ctgccctata
     1081 aaaggagggt atgcaccacg atggttcatt acccaataag attgaaagct caccactttg
     1141 ttgaaattga cagcaaacaa acaaaaaaat gcatttcacc ctttgacatc accatgcact
     1201 gccattaata tgcgccccgt tcacacgatt cctctgtagt tcagtcggta gaacggcgga
     1261 ctgttaatcc gtatgtcact ggttcgagtc cagtcagagg agccaaattc aaaaaagcct
     1321 gctttctagc aggctttttg ctttctaatt accaacgctc ttaaaacatc tgtcttgaac
     1381 cagaactaat ttgcacaggc attcccgatc gacgttgcaa cgcagcattt gcgcgattta
     1441 catcaacttc ttgcccgctg ataaacgccc gcaaagatgg ggttaccggc aatggcactt
     1501 ttcggtcaga ctcatattct gcacgattgc gcgacaacgg ctcatgaact tccagccagt
     1561 tcgagccatc tggttcagtg gtgtatttta ctggctggtc gataatttgc acacgcgtcc
     1621 caacaggaac attatcaaac agatatttga tatcgtcatt gcgcagacga atacagccct
     1681 gacttacccg gagcccaata ccaaaattgg cattggtacc atggatggca tacaacctgc
     1741 caatataaat cgcgtacagc cccatgggat tatcggggcc cgcaggaaca aatgcgggca
     1801 aactctcccc tcgtttcgca tattcgcgcc gagtgttcgg cgttggcgtc caggttggag
     1861 cttcttgttt acgttcaacg gtagtcaccc agttacgcgg ggtttctcgc ccagcctggc
     1921 cgataccaat aggaaagact tccacagtat tactgtctgg tgggtagtaa taaagacgca
     1981 tctcagcgac gttaaccaca atccctttac gaacagtggc gggcaaaatc agttgctgcg
     2041 gaatggtgag ttgcgagcca gacttcggca aaaaaaacat cagcgcccgg gttcgcttcc
     2101 agcatgttac ttaacccttg cccgtattgt gcggcaaaag tctccagcgg ctgggtattg
     2161 tgatcaggaa cagttacagt aagcgactgc cccactaaac ggctaccctc tggaggtaat
     2221 ggataagtta ccgccaggct attatggctg gcaaaaagca gagcaaatga gcaaaaaata
     2281 tttacacgac gcatcatgtc cctttcctaa gtcgcgaaag ctatccgtta agtatagctt
     2341 ttatcagact tttcgttttt aactgttcaa atcagaagtc gtattccccg gtagaacaat
     2401 attactggca gcaagttcgc ccatgttgtt atatatcgca caggcagctt cgatgatggg
     2461 catcgccaga gctgcgccac taccctcacc taaacgcatc tccatattga gataaggctc
     2521 cagccccaaa tgcgacagcg caatacgcgc ccctttttct gccgacaggt gagaaggaat
     2581 gagatacggt ttgattgcag gagacatctg gcaggctgcg agcgcggcag cataagaaag
     2641 aaatccatcc agcaacacgg gtaaaccgca ggaagcagcg cctaacatca cgccagccat
     2701 tccgaccaaa tcaaatccac ccacttttgc caggacatca acgccatcct gcggatttgg
     2761 ttgattcaac gtaatcgccc ggcgcacaac atcaatttta ttagccagtt tatctgtcgg
     2821 caggtttgcg ccaatcccaa ccacttcttc aggagcccgg ccagtgattg tgctgactat
     2881 tgccgctgcc ggtgtcgtgt ttgccatccc caggtcacct acaccaaaca gcgtaacacc
     2941 gttttttgcc agctcccgcg tataacatat gacgtccaaa agcaactttt ctgccagatg
     3001 gcgactcatt gccggagctg aagcaatatt gccactacct cgagcaacac gcatgttgat
     3061 aagcccgggg ataggctcag cagtatcaat accaacatca attacgtaga cgttagcgcc
     3121 cgcttgtgct gccagcacac acacgccggt tgttccgcgg gtcatatttt cagcctgaat
     3181 ggctgtcact tcttttgggg aaatagcgac cccttcctcc cagacgccgt gatcggcaca
     3241 catgaccagt accgcttttt tgcccacatg cggaacgcca ttcaaccccg gcattcctgc
     3301 cagttgtatg gcaagcgcct ccagccttcc caggctacca acaggtttga gtaacccgtc
     3361 gatatgccgt tgtgcacgcg acatagcggc aggatcaata gcagggatcg tattcagtaa
     3421 atcggcaagt gtttgcatgt cacgtctctt tatagcagtg ccagcaggaa gaccagttca
     3481 ccaagctcta tagcagctcc cagcgtatcg ccggtttgcc cgcccagcgt acgtttgaga
     3541 agctgaccga ggatgaaaat cgccaccatc gtcaccacca tagcagccac accatgcata
     3601 cccggcaata atacggcagc aaaaatagcg gctaagccga gtgtgacgca ggtttgtcgc
     3661 ccatcaattt tgccaatgaa cacattgcca agtccttctt cccgggcata acgatgacga
     3721 tacatcaata atgcggcagt cccgcggctg accgcacatg ctgccgccag cgatgcaagg
     3781 atcggttcgc cacgcagagc caactcgctt aataccagaa tctttgccag tacaacaaaa
     3841 atcaatgcca gaccgccgtg ggtgcctaaa cggctatcac gcatgatttc caacatgcga
     3901 tcgcggctac gtgcagaaaa tacgccatca caggtatcgg cgaggccgtc caggtggaat
     3961 cccccggtca tcagcgccag cacaagtacg ctaaacagtg ccgccagtgg tacgccacac
     4021 catgcctgca gcaccatgaa gaccagcccg ctaatcgcgc caagcaataa tccaattaaa
     4081 ggaaaagtaa taataccgcg agaataatgc tcgaaatcca gtccctggga ccagcgacgc
     4141 ggtacaggca gacgcgtaat aaaagagagc atcgcccaaa ataatttact catttgattt
     4201 ttactccaat acccgaaacc accaaccata cttcatttgc cgcagcggcc agctgctgat
     4261 ttacccgccc ggcaatatca cgaaaatgtc gtgccagacg actctccggc acaatcccca
     4321 ttcccacttc gttagtcact aatacaacct ttgcgggaca acgttggcag gcagcaatca
     4381 acgactgaat ctcagcatta atcgcctgtt ccatcgcctg ataatcccat tcatcagggt
     4441 ctttatcgcc gccataatca aacaacagat tagtcaccat ggtggtaacg cattccagca
     4501 atacagcctc atgcgggtta atgtctgcat gaattaattc atcaagatgt tgccagcgct
     4561 ccactgtgtg ccagtgcgcc gggcggcctt gccgatgatg ttctatccgt gcagccatct
     4621 catcatcaag gatttgtgag gtggcgatat acagaacctg cgaagagtcc ccaataagcg
     4681 cctctgcatg gcgactcttc ccgctccgtg cgccccccgt caccaaaatc atcataccga
     4741 ctcctgattc aattgtatga tggtattcat tgaacagaac aatggatccc ccaccgttgc
     4801 tgaccgcaaa acatttacgc gattaatact gcgcgtaata taattaaaaa tccagcattc
     4861 tcaatccatt acgaaagaag atggcattgc ggaatagcca gacttcctcg tgttaacgtc
     4921 cgacaaaatg cgtcattgac agcggtttct ctggaatgac agatttcatt cagataatgg
     4981 attaatgcta cagccccacc agccggacca gtcagcaaac gggcattgtg ttctgcaatc
     5041 aaaattaccg tcgttccctc agaatgagcg agccattctg gtacggatat ctcgctttca
     5101 gctgctatca atagcagcac tgaataatcg ctatacgcat ctgataacga ttgcacaggc
     5161 ccaccatcaa gaggctggaa atcttgctgc cagtcctcac caacaaaaca ggttattggc
     5221 aatatttcag ccagatgttt cgccagatcc acatctccag ataagaggca aaaaagagct
     5281 atcgattcgg gttttatcgt tttcatgcct ctcgccctcc acttaatttc ctgtaatggc
     5341 ggtctggttg ataagccatg aaacaatatt gccacacctt gaatgcagcc ctggtgcctg
     5401 tcggcactgc gatgaaagaa ctaaatgatg ccgccatacc ctgatccttt cagggtggta
     5461 ggggtataaa gacgcgccag tactctaccg gacgcttccg ggcaacccac ttcccaccga
     5521 agttgttggt attgactgac agactggtct tctggcttag cgtcatcctc acccgacctt
     5581 cccaatctcg cgatcagtgg cattttcggg tttgtcagca ttacagcagc gggggctgcg
     5641 agggattttc accctcttcc caggcacaaa tgtgccacac ctgtcagcgt atgttatgag
     5701 ctgatttcac ttttcccata acacgtaaat aacgttaatt attcattcca gcgtttattt
     5761 aaaccttccc tttttttagc atttattcta taccgtttcc atcgccgcat tttcactcac
     5821 ttgctgtcga tacacctgcg gcgtaacctg ataagtctga cggaaaactt tgcaaaaata
     5881 actgtattgt cgtggggatt aggcgcacga gtctggcgca ccgaatctga ccgagatttg
     5941 ttctctttaa taaccacaaa attgagttgt tttttgaggc aattttccac aatcaacttc
     6001 aacaaatcag cagaggccat aactcttgag taatccatct caggaacttt gcggaattca
     6061 ttaagtaatt cgggatctgc ctgccagcaa tcatcgacat tcagaatatc gaccaaatcg
     6121 acatcatcat tacgcagacg aacttgcccg cacaatacaa aaccgactaa atgtccggcg
     6181 attaccagtg gaatagagaa atcggttaat ccggcatgac agcgataaat acaaggctga
     6241 tcggatttag aggcttccag gcccccacaa cgatcgctca tgcgacatcg agtgcttagc
     6301 tgagggtgtt gccgcatcat ctgacaaaat ggagtgaagt taaacagctc ggaaatttca
     6361 tcgccgtgaa tattgaccac gacaaccgca aggctggtcg cctgcgcaaa atcctgcgcg
     6421 attttattaa tgagttctga attaagtgtc gccgcagaaa tcatgataaa acctcccagt
     6481 taatttattg ttatagcaaa acaattgttt tacatttaat cttcacatca tccctgatga
     6541 tttactgacg agaagcacaa aggacaataa caaaagcctc cgtgaagcgg gagacttttt
     6601 aataagttcg gaaagcagat cacaaacagt aaaaaaataa atgtttataa ctgccagagt
     6661 atttcgttat tcgctaattc ctttaggtgt aacactattg acaggtaaat tctttgcaat
     6721 gagatagcga taaatcgccg caccaacaca tgccccaatg atcggtgcaa taatgggtac
     6781 aatgaaataa ggaatattgc gtccaccagt catcgcaatt tctccccacc ccgcaagcca
     6841 ggtgaaaatc ttaggtccaa aatcacgcgc tggattcatg gcaaaaccgg ttaatggtcc
     6901 ggtagaagca ccaataacag caaccagaat accaataagt aaaggtgcaa gcggtccttt
     6961 gggtacgcca ttaccgtcat cggtcaacgc cataatcaac cccattaaca tggacgttat
     7021 gaccacttcg accaacgcag cttgccatac attaatcgcc gctgccggat aagtactgaa
     7081 gatactggcg agttgtaaac tttctgcgct accacgcacc atattgtgag cagattcaaa
     7141 atcggtaaac agactactgt atagagaata tgccagaact gcaccaccaa aggcacccgc
     7201 taattgcgcc agacaaaatg gcagcacttt acgggctggg aaagaggcaa acagccacag
     7261 agcaatggta actgccgggt tgagatgagc gccggaaata cctgccgtaa ggtaaacagc
     7321 aagcgaaatc cctaaccccc aaatgataca aatttcccac agtcccagac ttgccccggc
     7381 aactttcagg gcgcacaaac aaccaatgcc aaaaaagagg aacagtccgg tgcccaaaaa
     7441 ctcggcaacg cattgcgcct tgagtgaatc attcatagtg acaccttttt tacggagttt
     7501 ttctttatca gccaactatg cgctgactgt tgtatatacc gagcacaact ataaaaaatt
     7561 gctctgcaga aaatattgcc aacatgcatg acagactcgt gtaaaaatcg tgctaacata
     7621 acaaattatt gtcattaccg aatcatgcaa cacagcgtta gtcagtgttg aaagtaataa
     7681 ttcccaccaa acttttacaa taaaaaattg ccatcatgac ataataaaat tgaaacaaat
     7741 atcacagttc cttcttcgaa ctaaactcaa ccaaatatta ctcgcgatta aactcactag
     7801 ccaacgcaat taattcacat tgaaatatcc acaaaacaac caataaatta acaacatata
     7861 gttcataaaa gcaattttat aatatttgtt ctctattaat ttctctcatt agccgattaa
     7921 gtcagccatt ctttttcctc gctttttttt atagtcatat cgtcaaaaaa atccaggcga
     7981 ggtctttatg caacaagaag cgttaggaat ggtagaaacc aaaggcttaa ctgccgccat
     8041 agaggccgca gacgccatgg tgaagtcggc aaatgttttg ctggtcggct acgaaaaaat
     8101 aggttccggg ctggtgaccg tcatcgttcg tggtgatgtc ggggcggtaa aagcggcaac
     8161 agacgcgggt gccgcagctg cgcataacgt cggcgaagta aaagccgtac acgttattcc
     8221 ccgcccacat accgatgtcg aaaaaatctt accaaaggga attaacccat gagcagcaat
     8281 gaactggtcg aacagatcat ggcgcaggtc atcgctcgcg tagcgacgcc agagcatgag
     8341 gttctcccga aaaccactca tcaaatacga gagacggcta tggcagaaaa aagctgcacg
     8401 ctaacagaat ttgttggcac cgctattggc gacactgtag gtctggtcat tgcgaacgtt
     8461 gatagcgccc tgttggacgc gatgaaactt gaaaagcgct atcgttccat tggcattctg
     8521 ggtgcgcgta ttggcgcagg gccacacatt atggccgctg atgaagcggt aaaagccacc
     8581 aacactgaag ttattagcat tgaacttcct cgcgatacta aaggcggtgc cggacacggc
     8641 tcactgatcc tcttaggtgg caacgatgtt tccgacgtga aacgcggtat tgaagtcgcc
     8701 ctgaaagaac tcgatcggac ctttggcgat gtctatgcca acgaagccgg acatcttgaa
     8761 ctgcaatata ccgcccgcgc cagctatgca ctggaaaaag cgtttggtgc accactggga
     8821 cgggcttgcg gcgttattgt cggcgctccg gctgctgtgg gtgttctgat ggccgacacc
     8881 gcgctgaagt ccgccaatgt ggatgttgtg gcatacagct ccccagctca agacaccagc
     8941 tacagcaatg aagtcattct gattatctcg ggtgactccg gcgcagtccg ccaggcggtc
     9001 atctccgcac gcgaaatcgg taaaaccgtg ctttcgactc tcggcgatac accgaaaaac
     9061 gatcaccctt cctacatctg aattctctga ggcagattta tgagatcgaa aagatttgaa
     9121 gcactggcaa aacgcccggt caaccaggac ggtttcgtta aagagtggat tgaagaaggc
     9181 tttattgcga tggaaagccc gaacgatcct aaaccgtcaa tcaaaattgt taacggcgtc
     9241 gttaccgaac tggatggaaa acccgccagc cagtttgacc tgatcgacca ctttatcgcc
     9301 cgctacggca tcaatctcgc gcgcgccgaa gaagtcatcg cgatggattc ggtaaaactt
     9361 gccaatatgt tgtgcgatcc aaatgtcaaa cgtagcgata tcgttcccct taccacagca
     9421 atgacgcctg caaaaatcgt cgaagtggtt tcgcagatga acgtggtaga gatgatgatg
     9481 gcgatgcaga aaatgcgcgc ccgtcgaaca ccatcacaac aggctcacgt caccaacgtc
     9541 aaagataacc cagtacaaat tgccgccgac gcagctgaag gcgcatggcg cgggtttgat
     9601 gaacaggaaa ccaccgttgc cgttgctcgc tatgcaccgt tcaacgccat cgccctgctg
     9661 gtcggctcac aggtgggacg tccgggcgtc ttgacccagt gttcactgga agaagccacc
     9721 gaactgaaac tcggtatgtt aggccacacc tgttacgccg aaacaatttc tgtctatggt
     9781 acagaacctg ttttcaccaa tggtgatgat accccgtggt caaaagggtt tctcgcgtca
     9841 tcctatgctt ctcgtggctt aaaaatgcgc tttacttccg gctccggctc tgaagtacag
     9901 atgggttacg cggaaggcaa atcaatgctt tacctcgagg ctcgttgcat ttacatcacc
     9961 aaagccgcag gcgttcaggg tctgcagaat ggttcggtaa gctgcatcgg tgttccatca
    10021 gctgtgccat caggcatccg cgcagtatta gcggaaaact taatttgctc atcgctggat
    10081 ctggaatgcg cctccagtaa tgaccagacc tttacccact cggatatgcg ccgtaccgca
    10141 cgtttcctga tgcaatttct gccaggcacc gactttatct cttccggttt ttccgctgtg
    10201 ccgaactacg acaacatgtt tgctggttca aacgaagatg ctgaagactt cgatgattac
    10261 aacgtcattc agcgtgacct gaaggttgat ggtgggctgc gaccagtacg cgaagaagat
    10321 gttatcgcta tccgcaataa agccgctcgc gctttacagg ctgtctttgc cggtatggga
    10381 ctaccgccta ttaccgatga agaagtcgaa gcggcaacct atgcccacgg ttcaaaagat
    10441 atgcctgagc gcaatattgt ggaagacatc aagttcgccc aggagattat caataaaaac
    10501 cggaacagcc tggaagtggt gaaagccttg gcgcaaggca gttttaccga tgtcgcccag
    10561 gacatgctca acatgcaaaa agcaaaactg accggcgatt atctccatac ctcagccatc
    10621 atcgtcgatg acggacaagt gctctctgcg gtcaatgacg tcaatgatta cgccggaccg
    10681 gctacaggtt accgcctgca aggggaacgc tgggaagaga tcaaaaatat tcccggtgca
    10741 cttgatccca acgaaattga ctaagggtga gaaatggaaa ttaacgaaaa attactgcgt
    10801 cagattatcg aagacgtgct ggcagaaatg caacccagcg acaagtccgt ttcatttcgc
    10861 gcacctgtct ctgcaactgt cccttccgcg ccagataccg gaaattttct gaccgaaatt
    10921 ggcgaagccc aacaaggcac tcagcaagat gaagtcatta ttgctgtagg cccggcgttc
    10981 ggcctggcgc aaaccgtcaa tatcatcggt attccgcata aaaacatctt gcgcgaagtg
    11041 attgccggta tcgaagaaga aggcattaaa gcccgtgtaa ttcgctgctt taaatcgtcc
    11101 gatgtcgcat tcgtagcagt cgaaggcgat cgtcttagtg gttcaggtat tgctatcggt
    11161 attcagtcga aaggcacaac ggttatccac cagcagggac ttcctccctt gtctaacctg
    11221 gaacttttcc cacaagcgcc tttgttgacg ctggatactt atcgtcagat tggcaaaaac
    11281 gccgcgcgtt atgcgaagcg cgagtctcca caaccggtgc cgaccctcaa cgatcaaatg
    11341 gcgcggccga aataccaggc caaatctgcc attttgcata ttaaagagac gaagtacgtg
    11401 gtcactggca aaaaaccgca agaactgcgc gtgacgtttt aacaaaggaa accttcatga
    11461 ataccgatgc aattgagtcg atggtcaggg acgtgttgaa ccgcatgaac agtttacagg
    11521 atgcagctcc tgtttctgcg gtccccaacg catcaatact gagcgccaaa gtgacggatt
    11581 acccgctggc aaataaacat ccggaatggg ttaaaacagc gaccaataaa acgctcgacg
    11641 atttcactct ggaaaatgtg ctaagcgata acgtcaccgc cctggatatg cgcattaccc
    11701 ctgaaacatt gcgtattcag gctgcgattg ccagagatgc tggtcgcgat cgtctggcga
    11761 tgaattttga acgcgccgct gagcttacct cagtaccaga tgatcgcatc ctcgaaatct
    11821 acaatgcgtt acgcccttat cgttcaacta agcaagaact tattgccatt gctgacgatc
    11881 ttgaacagcg ttatcaggcc aaaatttgtg ctgcatttgt ccgtgaagct gcggaacttt
    11941 atgtcgagcg caaaaaactg aaaggcgacg attaaggacg aactatgcga tatatagcag
    12001 gcattgatat cggcaactca tcaacagagg ttgcgctggc aacgctcagt gcaactggag
    12061 agttgtcgtt cgtgagtagc gccctggagg aaaccaccgg aatcaaaggt acgttgcgta
    12121 acgtccatgg tattcaggag gcgctcgcac aggcaacgaa aaaagtcggc atcaatgtta
    12181 gcgatatttc gcttattcgt atcaacgaag caactccggt cattggtgat gtggcgatgg
    12241 aaaccatcac tgaaactatc atcaccgaat caaccatgat cggtcacaac ccgaaaactc
    12301 cgggcggagt ggggctgggc gtagggttca ccatcactcc gcaagagtta ttaacacgcc
    12361 cggcggacac accttacatc ctggtggtgt catcggcatt tgattttgcc gatatcgcga
    12421 cgatgataaa cgcctccgta cgcgccggat atcagctgac tggcgttatt ctgcaacgcg
    12481 acgatggcgt actggtcaat aaccgcctgg aaattccttt acctattgtg gatgaagtcc
    12541 tctacatcga tcgtattccg ctcggcatgc tggcagcaat tgaagttgcc gttcccggga
    12601 aagttatcga aaccctgtct aacccgtatg ggattgccac cgtattcgcc ctcaatgctg
    12661 aagagactaa aaatattgtt cctgtcgcgc gggcgctaat cggcaaccgt tcagcagtgg
    12721 tagtgaaaac gccctctggc gacgttaaag cccgttcgat acccgccgga aatatcgaac
    12781 tgctttctgc cgggcgcaca acgcgcgttg atgtagctgc tggtgccgat gccattatga
    12841 aagcggtcgg tgagtgccca aaactggaaa acgtgacggg agaaccaggc accaacatcg
    12901 gcggaatgct ggaacatgtt cgccagacca tggcggaact gaccaacaaa cccagcaacg
    12961 aaatttttat tcaggatttg ctggcgatag atacctctgt ccccgtcagc gtaaccggtg
    13021 gtctggcagg tgaattttca ctggaacagg ctgtgggtat tgcctctatg gtgaaatccg
    13081 accgtctgca aatggcgatg atcgccagtg aaattaagca aaaactgcat gttgatgttc
    13141 aggtcggagg cgcagaagca gaagccgcca ttcagggggc cttgactacc cctggaacca
    13201 cacgtccact ggcaattctg gacttaggtg caggctctac cgatgcctcc atcatcaatc
    13261 agagtgggga aatcgttgcc actcaccttg cgggagcggg cgacatggta acgatgatta
    13321 tcgctcgtga gctgggctta aatgatcgtt atctggcaga agaaatcaaa aagtacccgt
    13381 tagcaaaagt agaaagcctg ttccacttac gccatgaaga cggtagcgtt cagttcttcc
    13441 ctactcccct atccccccat gtatttgccc gcgtctgtgt agtgaaaccc gacgaactgg
    13501 tgcctattcc cggtgatttg acgctggaaa aagtacgcgc tgttcgtcgc agtgcgaaag
    13561 agcgtgtctt cgtaaccaat gccctgcgcg cgctacgtca ggtaagccct gccggaaata
    13621 ttcgcgacat tccgttcgtc gttctggtgg gtggttcttc gctggatttc gaagtaccgc
    13681 aattggtcac cgacgcgctg gcacattatc gactggtcgc cgggcggggg aatatccgtg
    13741 gtagcgaagg tccgcgcaac gcggtagcta ctgggctact catcgcctgg cacaaggagt
    13801 cgattcatgg aaagtaaaga gcgtgcacca gccatcgttg tcatggagat cggtgactgt
    13861 atcactacat gggatgaggt gcttcttggc attgaagagg aaggtattcc ctttcgcatt
    13921 caacacattc cgtcaggtga agttatcgat agcgcctggc tggctgctcg tcaatctccc
    13981 ctgttggttg gcattgcctg cgaccgggaa gaactgattg tgcattacaa aaacttaccg
    14041 gcatcagcgc cactgtttac gctgacgtat caacaagata accatacccg gcgcagcatc
    14101 ggcactaacg ctgcccgcct ggtaaaagga attcccttcc gggaattcca ctcataatcc
    14161 acaggagaaa cgctgtatga acaacgcact aggactggtt gaaacaaaag ggttagttgg
    14221 cgcaattgaa gctgcagatg ccatggttaa atccgccaac gtgcagctgg ttggctatga
    14281 aaagattggt tcgggtctga ttacggtgat ggtccgcggt gatgttggcg cagtaaaggc
    14341 cgccgttgat gcggggagcg ctgcggcaag cgccgttggc gaagtgaaat cctgccatgt
    14401 catcccgcgc ccacacagtg atgttgaggc tattttacct aaatcggcat aaccccattt
    14461 gctaaggagc acagcgtgaa gcaatcactg ggattacttg aagccagtgg tctggctctc
    14521 gccatcagtt gcgcagacgc tatggcaaaa gccgcgtcca tcacgctggt tggccttgag
    14581 aagaccaatg gctcaggctg gacgctcatc aaaatcaccg gtgacgtcgc ctcggttaat
    14641 gcggcgatcg cgacgggagc cagtttcgcc ggacagcgaa atggccttgt cgcacacaaa
    14701 gtgattgccc gcccgggaga cggcattctg ccccacacaa ctgaagcatt gaccgtgacg
    14761 gttgaaacaa gcaaaacacc agcaacaacc gtcacctcag agattatcag ttgtaatttg
    14821 tgtcttgacc cggcatgtca gcggcaaaaa ggtgagcccc gctcactttg cctgtacgcc
    14881 aataagcgag gggagtagca gtggataaac aaaccttgca ggaaacagtg agccagattc
    14941 tggatgagat gcgcgagcgc cctatcccac tgggcgtttc cagtcgccat atacatcttt
    15001 catcagcgga ttatttgcgt ctttttcctg aacaaccgat tagcgagaaa aagccgttaa
    15061 tgcaacctgg ccaatacgct gctgaacagg ttgttacgct ggagggacca aaaggcaaat
    15121 tgcacaacgt tcgcttgttg gggccgctac gcaatgcaag ccaggtggaa atttcccgga
    15181 cggacgccag aacgttaggg atcaccgccc cgctacgcat gtcaggcgat ctcagcggca
    15241 ctccggggat tcgtttgata agcccatttg cggaactgga gatcacatct ggcgtcattg
    15301 tcgcgcaacg gcatattcat atgtctccgc tggatgccct gatttatggc gttacgcatg
    15361 gcgataaagt ggcggttgcc attgaaggtg atgagcgacg actgatattc gaaaacgtcg
    15421 ctgttcgcgt ctctccggat atgcgtcttg aaatgcatat tgataccgat gaggccaacg
    15481 ccgcaggcgc tgatagccct gacgtttttg ccacactggt gtcagtgcca tgaataacat
    15541 gctgatgcaa cgtattgttg acgaagtggt ttttcgcctt aaacaacgtg cgggaaaaac
    15601 actcgtgctg actgtttttc aattgcgaga cgccagcgtt caagagagcg ttcatcagta
    15661 tgcgtcatta caaatccgat atgtcgatct gccgctgctg cgacaacttg cggagaacga
    15721 aacgtcagat cgagcatcta tccagataca cgaagcgtta gcctggggaa tgcacattca
    15781 attgtcgttg cagcgtcatt tcctgaacgc catcgaattg aagactctcg cccgtctacc
    15841 gctaagctgg tgcgacgaac aagggcagcc gatctacctt catcataatc gacttttaag
    15901 ttatgccgat atcgcacagt taagctcggg aatactggtg ttacagcgga aatgttgtgt
    15961 gaccgcactg gctcgtgaag cggcaattac ccgaaatatt cagttaatca ggcaggagta
    16021 acctatgcat ctggctcgag tcactggcgc ggtagtatcc acgcaaaaat caccttctct
    16081 gatcgggaaa aaactgctgc tggttcgccg ggtcagtgct gacggcgaac tgcctctcac
    16141 gccattaacc ggcgacgaag ttgccgttga ttcagtcggc gcaggcgttg gtgagttggt
    16201 actcctcagc agtggttcca gcgccagaca tgtgttttca ggccctaacg aggccatcga
    16261 cctggcagtg gtcggcattg tcgatacgct ttcgcgctaa ggagctaaaa gatggcaatt
    16321 tatacccgaa caggtgattc aggaagtacg tctttattta ccggacagcg cgtcagcaaa
    16381 actcatctac gtgtcgaaac ctatggcacg ctggatgaac tgaacgcgac actcagtctg
    16441 tgttattgcg ccaccgccat cgaaagccat cgcatcttac tggaagccat tcaacaacag
    16501 atattttggt ttagcgccga actcgccagt gaaagcgaac agccgtcagc gcaacagcgt
    16561 tacatcggta cggaagaaat tgccgcgttg gaaaacgcga tcgatagtgc aatgaacgcc
    16621 gtccccccag ttcacagctt cattctgcct ggtcgatgtg aagccgcaag ccgcatgcat
    16681 ttcgcccgca cggtggctcg acgggctgaa agacggctgg ttgaactgac aacggaaacc
    16741 accgtccgga atgttttgct gcactatatc aaccgacttt ccgattgcct gtatgccctc
    16801 gcccgcgtgg aagacaacgt tgctcatcaa aacctgatga ttcaggaaat cacaaaacgt
    16861 tatcacgaag ccaaccacac accagcattg aaggaacgca caatgccgct cacttttcag
    16921 gatcttcacc agctcattcg tagtgcggca atgcgcgcgg atgaactgca tattcccgtt
    16981 gtcatcagca ttgttgatgc caacggaaca gaaagtgtta cctggcggat gcctgatgcc
    17041 ctgttggtca gcagtgaact ggcaccgaaa aaagcctgga ccgccgtggc gatgaaaacg
    17101 gcaacccaca agcttgctga taccgtgcaa cccgacgcgc cgctttacgg gctcgaaagc
    17161 catatgcaag gaaaagtcgt cacttttggt ggcggcttcc ccctctggcg tgacggaaaa
    17221 ttgcttggcg ggcttggcat cagcggcggt agcgttgaac aagacatgga tattgcccaa
    17281 agcgcaatgg cggcaattaa cgtgggagta aaccaatgaa taactctgaa ttagaaacac
    17341 tgatccgcaa tattctgcgt gagcaacttg tccccgctac accacgaacg caaagaaacg
    17401 ctattttcca gactgtcgat gaagcggtct gcgcagcaca ctaggccttt ttacgctttc
    17461 aacagtgccc gcttaaaacc cgtagcgcca ttatcagcgc cattcgcgaa gaattaatgc
    17521 cccgccttac cgaactggca gaagaaagcg ccaaagaaac ggggatggga aataaagaag
    17581 ataagtacct aaaaaacaaa gcggcactcg ataacacgcc aggaattgaa gatttaacca
    17641 ccacagcact aaccggtgat ggtggcatgg ttttgtttga gtactctccg tttggggtga
    17701 ttggctcggt cacgccaagc accaacccca cggaaaccat cattaacaac agcatcagca
    17761 tgctggcagc aggtaatagc gtctacttca gtccacaccc gggtgccaaa aacgtttcgt
    17821 taaaactgat taccacaatc gaagacatcg cgtttcgctg tagtggcatt cataacttga
    17881 tcgttaccgt atctgaaccg acctttgctg ccaccgaaca gatgatggcg caccctaaaa
    17941 tcagcgttct ggcgattacc ggcggtccgg ggatcgtcgc catgggaatg aaaagtggca
    18001 agaaagtgat tggtgccgga gcgggtaacc ctccgtgcat cgtcgatgaa acggcggata
    18061 ttgtgaaagc cgccgaggac atcatcaacg gcgcttcatt tgattacaac ctgccgtgta
    18121 ttgctgaaaa aagcctgatt gtcgtggcat cagtggcaga ttatttaatt cagcagatgc
    18181 aaagttttgg cgcacttctt cttaactatg agcaaactga gaaattgcgt gcaatttgcc
    18241 tgccagacgg tagcgccaac aaaaaactgg ttgggaaaag cccatcagcc ttgctggaag
    18301 cggcaggcct ccctttgccc gcaaaagcgc cgcgcctact gattgctgtt gtcgacgccg
    18361 atgatagttg ggttacttgt gagcaactaa tgccaatgct gcccattgtg aaggtcaagg
    18421 attttgacag cgctctaacc ctggcgctga aagtggaaga cggcttgcac cacacggcaa
    18481 ttatgcactc tcagaacgtc tcacgactca accttgccgc gcgcgttatg caaacctcaa
    18541 tatttgtcaa aaacggcccc tcctgggcgg gtattggcgt cggcggcgaa ggctttacga
    18601 cctttaccat tgcaacaccg actggagagg gtacaacgtc agcacggact tttgcccgct
    18661 cacgacgctg cgtgttaacc agcggctttt caattcgcta gagaaacgga aatgaatact
    18721 ttttcactgc agacgcgttt atacagcgga ccaggaagtc tcaaggtact tagccgtttc
    18781 tctcatagac acatctggat tatttgcgat gcctttctgg cacgctcacc acttattgac
    18841 acgttgcatc aggcgctgcc cgacagtaat cgtctcagta tttttagcga aataacgccc
    18901 gccccaacta tccagaccgt ggtgaaaggc attgcgcaaa tgcagacttt acgcccggat
    18961 gtagttatcg gttttggcgg cggttctgcc ctggacgccg caaaagcaat tgtctggttc
    19021 ggtcgccagt gtggcattga aattgaaacc tgcgtagcca ttcctaccac cagcggtatc
    19081 ggttccgaag tgaccagtgc ttgtgtcatc agcgatcctg agaaagggat taaatatccc
    19141 cttttcgata atgcacttta tccggatatt gcgatccttg atccatcttt ggtcgtcagc
    19201 gtgccaccgg cgataacggc caataccggg atggatgttt taacccatgc gcttgaagct
    19261 tatgtttctc ctcgcgccag cgacttcacg gatgcactgg tcgaaaaagc cgtgcaaatt
    19321 gtttttcaat acctgcccac tgcagtgaaa aaaggcgatt gtcttgccac ccgtggcaaa
    19381 atgcataatg catcgacgct tgcaggaatg gcctttagcc aggccggact cggtttaaac
    19441 catgcaattg ctcatcaact cggcggacag tttcatctgc cgcatggact ggcgaatgcg
    19501 ctcctgctca cggcagttat ccgctttaat gctggcgatc cgcgcacagc caaacgctat
    19561 gcccggctgg cgaaaacgtg ccatttatgc ccggacaacg ccaatgacac cgccagcctg
    19621 aatgcactta ttcaacatat tgagcagctt aatacaacct gtacgctgcc gactcttgcc
    19681 aatgcgctga aagaaaaaaa agcagaatgg tcaatacgca taccagatat ggttcaggcg
    19741 gcgctggcgg atgcaacgtt gcgtaccaac ccgcgtgcag ctgatgcctc cgcgattgca
    19801 gaactgctca aggggttgtt atgaacgtcg gattaatctc ctgcgacgac cagaccatcc
    19861 gcgagcgagt acaagcagca ggtgtcgtgg gtgctggcgg cgcaggtttt ccaacccata
    19921 ttaagctaca agcgcgtgct gacactttcc tggtgaatgc ggcggaatgc gaacccatgt
    19981 tgaaagtaga tcaacagtta atggcgcagc aagccagccg cctgcttcgc ggtgttcact
    20041 atgcgatgaa agccaccggg gccagtagcg gaatcatcgc gctgaaagaa aaatatcaac
    20101 gggcaataaa cgcactcaca ccactgttac caccggatat ccgcctgcat attttgcccg
    20161 acgtttaccc cgcaggagac gaagtgctga ccatctggat ggcaactggt cgccgcgttc
    20221 cacccgctgc attacccgtt agcgtcggtg tggtggtcaa taatgttcaa accgtactta
    20281 atattgccag agccgtcgag caacagtatc cagtgacgcg ccgcacactc accgttaatg
    20341 gtgcagtggc gaagccaata acggtaaccg tccccatcgg catgtcttta cgtgaagtcc
    20401 tggcgctggc gggcggcgca acagtggatg ctcctggttt tatcaatggc gggccgatga
    20461 tgggaagttt gatcacatcg ctcgacacac cagtcagcaa aaccactggc ggcttgctgg
    20521 ttttacccaa ctctcattca ctgatccaac gtcgaatgca gaatgaccgc agcgtacttg
    20581 ccgtcgcgaa aactgtttgt gagcaatgcc gcttgtgtac cgatctctgc ccacgtcatt
    20641 taatcggtca cgaactggca ccccatttat tagtacgtgc gcggttaact accaacagct
    20701 cgccacgccg caactgttac tgacagcgct cacctgttca gagtgcaacg tctgcgccag
    20761 tgtcgcctgt ccggtaggaa tttcaccaat gcgcataaac cgcctgctta agcaggaatt
    20821 acgcgcacaa aatctccgct acgaaggtgc cttaaatcct gcagatccca tggcaaatta
    20881 tcgactgatt cccgtaaaac ggctggtaac cagacttggc ttaaccccgt ggtatcagga
    20941 tgcgccattg tctgaacagg tgcctcagcc cgaaaaagtg acgttattgt tacgccagca
    21001 catcggtgcc agcgcaattg cgtgtgtaca aaaaggcgat cgcgtcgttc atgggcaatg
    21061 tgtaggtcaa attccacacg gcacattagg tgcccccatt catgccagta ttgatggcat
    21121 ggtcagcgat gtgacagaaa acgcgattac gcttgtgaga ggttaaccat gtcgaaagcg
    21181 gtaggtattt tagaacttag cagtatcgcg aaagggatgg aaaccggcga cgccatgctg
    21241 aaaagcgcca acgttgagtt actggtcagc aaaacgctat gccccggaaa atttctgctg
    21301 atgctcggtg gcgatgtggg cgcggtgcaa caggccatca acagcggagc gtcccttgct
    21361 ggtgaaatgc tggtcgacag tcttgtcctg ccgaatattc atcccagcct cctttctgca
    21421 atcagcggac ttaaccatgt tgaacagcac cgggctgtcg gcgtagtcga aacctggagt
    21481 gtcgccgcct gcattgacgc tgccgattgt gctgtcaaag ctgcaaatgt cattcttgtt
    21541 cgcgtacata tggcttttgg cattggcggt aaatgctaca tggtggttgc tggcgatatt
    21601 tcagatgtta ataacgcggt ctctgccgcc agcgtgcgcg cgggggaaaa aggtttgttg
    21661 gtctatcggt cggtgattgc ccgtccacat gaagccatgt ggcgtcagat ggtggaggga
    21721 taatgacaac tcagcacaca actgaacgga tgattcagga atatgtccca ggtaagcagg
    21781 tcacgctggc acatgtcatc gccaatccag ggaaggatct gtttaagaaa ttgggtttac
    21841 cagacaccat ttccgctatt ggtattctga caattacccc cagcgaagca tctatcatcg
    21901 catgcgatat cgccacaaaa tcaggcgctg tagagatcgg ttttcttgat cgttttaccg
    21961 gtgccgtagt gctgactggt gatgtttctg cgatcgaata tgcacttagc caggttaccc
    22021 gtacgctggg tgaaatgatg cgctttaccg cctgccccat tacgcggacc tgacgttatg
    22081 caacgtatga tgctcatcgg ccccagccag tgcggaaaaa cgtcactcac gcaatgtctg
    22141 cgtggcgaaa atattcatta tcagaaaacg caggcaatcg tctggtcccc gaccaccatt
    22201 gatacaccag gcgaatacct cgaaaaccgc agcctctaca gtgcactgtt ggtcagtgcc
    22261 tgcgaagcgg acattatcgc gctggtgctt aatgcggacg caccgtggtc acctttttcg
    22321 ccaggcttta cctcgtcaat gaatcgcccg gtcattggag tagtaaccaa agcagattta
    22381 gccaacatgg aacaaatatc tttggtgaaa tgttggttac gggaagctgg agcgcacaac
    22441 gtgttagtaa ccagtgcggt taataaccat ggagttgcag aacttttagc cttgctgcat
    22501 acagaagaag gctgtcgcta atttcgacag gttatcagat gggaatgatg acggtgatac
    22561 acagaaaaga atcaatgagc aatgagggtt gccgggcaac cctcattgaa taaaacagga
    22621 attacttcgc ttcgccgttt ttcatttcgc ccatagcttt cagctttttg gagatatcgc
    22681 ggcgttcttt ggagagctcg gcatttttga tgatgtaatc gtcaacgcga tcttcatagt
    22741 cacctttcat gctggcgatg atgccctgga ttgcttcaac gctcatccca ggcttgatgt
    22801 aatcgctcag gttgtccagc agcaggacgc gcttctggtt gtcacggatc tttttctcaa
    22861 cgtcctgaat ttcacgttgc agtttgttct tacgacggaa cagacgaaca aattccagta
    22921 cgtcctggaa tgaaggcttg gtagtttcca tttttatacc cctgataatg tgagagtcgg
    22981 attcgtttaa gcaaccgcta ttcgttaggg ccaacattac tcatagctgc cgcagatgac
    23041 aatgctttta tcccttttca ctatcatacc ctttatcctt gctgaatcga agcagcagca
    23101 agatgattct gaagttcagg aattatttgc gcaaggcccg gcaaatcaga ttcgatagca
    23161 actcaagctg atgcgccgtt tccatgttca gccacacata accatagatt ggtgtttcca
    23221 ctaccttcag gtcatggtgg ttattgagca actgacgcag ctcttccaca gcatcgttca
    23281 atttttccgt attggcaaaa accggctgcg gattaccttc gtacagcgca tgaacaaagc
    23341 tcagcagtat ttgctgcatc atgtgctggg tatcacgaag tttttgcgcg ttcaataaca
    23401 cgaaatggct ggggcgcgtg gcccagtacg cattgatttg caactccagc atacaaacca
    23461 gattgcggtt aatggtctgg ataccttcat atatcgattt tggaatacgg gtttctttgc
    23521 tggcgggcgc aatcagccca cgcattttca cggcatcggt caggagtttt tgtagatggc
    23581 tttccagacg tgggcgttcg agtaagttcg gtgagaatgc agattgatag acccgattat
    23641 actcggtcag acttttcgcc agttgaatgc gccagtggat gaacgcccgt tgtggccaga
    23701 taccggtaaa caacattgcc agtaaagagc cgaggatcac atcgccgctt cgccataacg
    23761 ccgtatcaat ttcacctgtc ggggaaccaa caacaattgc cagcgtcacc ccaatcaata
    23821 atccttgata cggtttcttg cccagcgcca gccaaccgca aagaaacatc gccgccgcgc
    23881 accagactaa catcagcggt aacgaaatta actccagttg cagagcgata agacctaaaa
    23941 tcgaacccaa caccgtaccg ccaatacgtt cgaaggcacg agggacaacg ttaccccaga
    24001 acgagattgg ccccataatc accaccatgg tgaccagcgg ccaggtgctt tccgggatag
    24061 taaacaggcg gataatgaga aaagtgagca ggaatgccag cgcgacccga gtaccatgca
    24121 ctatgcggta atggcggtat acccggattt caaacgggct taatgactta tcggcacgca
    24181 cgcagaaact ccgtaatgaa gattaaaagc gctcataact catctgtcgg ccagaaggcg
    24241 cgcctctgaa ttatccgacg gacaaaccgg gaacagtgta atttcccggc aggagcgaaa
    24301 aaattgaaag gcgcatctta ctcttttcgc tttcaaaaaa agagtggtca tcgcgttaac
    24361 acaccgccct gagatgaatt agtgatgttt tggctgcacc gcaacataca tttcgatatc
    24421 ccagtaccca tcttccgcgc cattgttcaa ataaacctcg aagcagggct ttggtaacat
    24481 ttcataagca ctgtcctgca agaggctatt aaagaactga taccagggtt tagcaaaatc
    24541 atcaccgact acacgagcca ccgccaccgc atactgacca cctgaaattt ctgtcagaat
    24601 gacgccctca ctgttttcgg gaagcgtaaa gttattcggt accgtcacaa cggtgtcgca
    24661 gcgtaatttt tcggcgggtg tttcatctgg attgtcgtaa tagacagcaa cccactcctt
    24721 cggcacaata tttttgttat ctacccacat catcaactgc tcaaagcctt tctttaccgt
    24781 ctgttcccac gggccaacga gatggaaacc tgcaacggta cgtttctctt cctgcgtaat
    24841 ctcgtagttc atgacgcctc cattaacact gtttttatat acagcatagt tgcaatatta
    24901 aaaccacaag gaatgagtgt tgattatgcg agcagactcg cactcctgcc agtctgctgc
    24961 aaaagaaagg tcaggcctta tggtggaaat aatcactcag gcgggaaaac atgccgcctt
    25021 ccccgacaga ttccagggta accagcggcc agtgcgccac ctgtttatca cggtcgtaaa
    25081 gttcaatttc ccctacccgc tgatgggcgc taattggcgc ggtgagctct ttaccatcaa
    25141 gggtatattt ggctttgata tgtggaattt cggctttcgg tagcaccatc cagaactctt
    25201 gttccgttcc taggtcgata ttttctttat cgccatacca gatgcgttcc gttccgacct
    25261 ttttcccacg gtgcaaaatt tgcaccgtag taaagttttg ttgcccccaa cgcagtaatt
    25321 ttcttgcctc ttcctcacga ccttttgcac tgtcagcccc cattaccact gcaatgagac
    25381 gacgctgccc atctacagcc gaagcaatga gattaaaccc ggcaccagaa gtatgacccg
    25441 ttttcaggcc gtcaacattc atggttttat cccacaacaa cccgttacgg ttttgctggg
    25501 tgataccgtt ccaggtgaga cttttctcac tgtacatatg ataaaactcg ggctcgccgt
    25561 ggatgatagc gcgagaaaac acagctaaat cataagccga gctatgctgg ccaggtgcat
    25621 ccagaccatg cactgtttca aaatgcgtat ccttgagatg cagcttctcg gcatagttgt
    25681 tcatcatttc aacaaactgc cgttgcccac cggcaatata gtcagccaga gcaacacaag
    25741 cgtcatttcc ggaatccaca attaaaccac ggcttaaatc acgtaccgat acgcgatcgc
    25801 cctctttcaa aaacatcagt gaagaaccga caaacaccgg attatctttc gcccacgcat
    25861 cgcgccccac ggtgacaata tcgtctggcg taatgcgatg actatcgata gcgcgatcca
    25921 cgacataacc cgtcatcagc tttgtcaggc tggcgggatt gcgctgttga tgctcattac
    25981 ccgcggtgag gatctgaccg gtggtgtaat ccatcaatac ccaggacccg gcatgaatct
    26041 ctggaggctg aggtgaaaaa ggaatgtttt ccgccgcaaa accagacgat aagttaaaaa
    26101 cgaacaaaga agcagcaata ataagacggc gtttcaacag caaaccctca ggagtttcaa
    26161 atagctgttc tttttacgga aatacttatg aactggctgg aataaagtgc aagaaaatgt
    26221 gactaccctc tcatttttat ctgacatgat ctgttgccac tcgctgccaa attgtggcgc
    26281 taaagctgat tagcacggtg atatttgata ctctggcaga cagcagaaat aacggattta
    26341 acctaatgat gaatgacggt aagcaacaat ctaccttttt gtttcacgat tacgaaacct
    26401 ttggcacgca ccccgcgtta gatcgccctg cacagttcgc agccattcgc accgataacg
    26461 aattcaatgt catcggcgaa cccgaagtct tttactgcaa gcccgctgat gactatttac
    26521 cccagccagg agccgtatta attaccggta ttaccccgca ggaagcacgg gcgaaaggag
    26581 aaaacgaagc cgcgtttgcc gcccgtattc actcgctttt taccgtaccg aagacctgta
    26641 ttctgggcta caacaatgtg cgtttcgacg acgaagtcac acgcaacgtt ttttatcgta
    26701 atttctacga tccttacgcc tggagctggc agcatgataa ctcgcgctgg gatttgctgg
    26761 atgttatgcg tgcctgttat gccctgcgcc cggaaggaat aaactggcct gaaaatgatg
    26821 acggtctacc gagctttcgc cttgagcatt taaccaaagc gaatggaatt gaacatagca
    26881 acgcccacga tgcgatggct gatgtgtgcg ccactattgc gatggcgaaa ctggtaaaaa
    26941 cgcgtcagcc acgtctgttt gattatctct ttacccatcg taataaacac aaactgatgg
    27001 cgttgattga tgttccgcag atgaaacccc tggttcacgt ttccggaatg tttggggcat
    27061 ggcgcggcaa taccagctgg gtggcaccgc tggcgtggca tccagaaaat cgcaatgccg
    27121 taattatggt ggatttggca ggagacattt cgccattact ggaactggat agcgacacat
    27181 tgcgcgagcg tttatatact gcaaaagccg atcttggcga taacgccgcc gttccggtta
    27241 agctggtgca tatcaataaa tgtccggtgc tggcccaggc gaatacgcta cgcccggaag
    27301 atgccgaccg actgggaatt aatcgtcagc attgcctcga taacctgaaa attctgcgtg
    27361 aaaatccgca agtgcgcgaa aaagtggtgg cgatattcgc ggaagccgaa ccgtttacgc
    27421 cttcagataa cgtggatgca cagctttata acggcttttt cagtgacgca gatcgtgcag
    27481 caatgaaaat tgtgctggaa accgagccgc gtaatttacc ggcactggat atcacttttg
    27541 tcgataaacg gattgaaaag ctgttgttca attatcgggc acgcaacttc ccggggacgc
    27601 tggattatgc cgagcagcaa cgctggctgg agcaccgccg ccaggtcttc acgcccgagt
    27661 ttttacaagg ttatgcagat gaattgcaga tgctggcaca acaatatgcc gataacaaag
    27721 agaaagtggc gctgttaaaa gcgctttggc agtacgcgga agagatcgtc taatttgaag
    27781 cctccgccgc tggtacggcg gaggtcaatg ttgagtcagg ctttttgaac ggtgatactc
    27841 cacgctgcat cgccaatctg ctgataatcg gtgatggcat gcccttcttc tgccgcccac
    27901 tgtgggattg cttcagttgc ctgggtgcaa tcaaactcaa ttaccaactc atcgccgctg
    27961 gccatttctg ccagtgctgc ttttgcttct atgagtggaa acggacagac ctgcgtcacc
    28021 acatccagct ttttaatcgc catctttaca ccttacttaa tttgccgcag cagttgccag
    28081 tcgcgcctta cgctgcggtc gaacatacac aagccaggac gcagtccaga ctccgagaat
    28141 cataaataca aggccaatcc agccctgcca ggtcatcatt gcagtcataa ccagtccatt
    28201 accgataaag caaccacctg caatactggc accgaagccc atcagtacac cacctaaccc
    28261 gctacgtaat gttgtttgtg catcagctgc gcgaacgcgg aactcacggc tcgctttggc
    28321 tgcaataaat gaccccacga agatccctaa caccaggaaa acgccccagt taatgtattt
    28381 cacgtcaccc gcgaccagaa attgcagaat attggccgtt ggggaagtga ttcctaaccc
    28441 aaacatgcgt ccggttgctt cactcagggg ccaggctaaa agcgcaatca aaccgatgag
    28501 tacagcagtg acaaagggat gccagcgttt ttcaaacaga atatgagcga tcccagttcg
    28561 gcgcggcggt aaggtcgcga ctttgagttt tggcttcttc aactctttca tcaccaccca
    28621 gagcgttatc accagcaaaa cggcaaccaa cggccaaaca gacaaattaa aagtctcagc
    28681 aatagagtta tgttcagtac tgtagtgctg caaggtttga tttaaaccac tggcatgtgg
    28741 agaacgcatc accgcactca tcaccatata agtgaaaagc gcgatccaac tgccgatcaa
    28801 tccctcaccc gcgcgatacc aggtcccggt ggcacatcca cccgccagaa caattcccag
    28861 cccgaagata tacccaccta taacagtacc aagccacggg aacgcctcgg cttcgtaagt
    28921 cagtaggccc gcctgaatta acgcaaaaac ccccacgctt tgtaccgaaa ttgcaatcag
    28981 caaggcgtaa aacatgcgat tatttttcgc gatatacata tcgcgaaaac cacctgtcag
    29041 gcaaaaacgc ccacgctgca tgacaaatcc cagcagagca ccacaaatta gcccgcttaa
    29101 tatcattgaa aacataaatt aattaaccag atgaatgtta atgaggaaat tattcatgac
    29161 tggtggaatg cagaccaata accaaattct ctaaattaga acaaatggtt attaatgagg
    29221 atttcgataa aaaaaaataa aaaaaccgga gccaaatatt cattcgactc cggttgatta
    29281 ttagaaaaga tgattatgca acgtcttcgt actgcggaac tgggttacgg aagctcttgg
    29341 tgacgcaagc caggtaaatc agaccgatag cagcccaaat cagacccaga accattgagc
    29401 tttcttccag gttaacccac agcgcaccaa cggtcagcgc accacacatc ggcaggaaca
    29461 gatactggaa gtgatctttc agcgtcttat tacgcttctc acggatccag aactgcgaga
    29521 tgaccgacag gttaacgaag gtgaacgcca ccaacgcacc aaagttaatc agcgctgtag
    29581 ccattaccag gtcgaagttg attgccagca gggcaatcgc gccaaccagg atgatgttca
    29641 tcgccggagt acgccatttc gggtgtacat aaccgaagaa gcttttcggg aatacgccgt
    29701 cacgacccat tacgtacatc agacgcgcta cgcctgcatg cgccgccata ccggacgcca
    29761 gtacggtaat ggtggagaag atcagcgcgc caacctggaa cgctttaccc gcaacgtaca
    29821 gcatgatttc aggctgtgac gcatccggat ctttaaagcg agagatatcc gggaagtaca
    29881 gctgcaggaa gtaagttgca aagatgaaga tcatgccgcc aatcagcgcg gtcaggaaaa
    29941 tcgcacgcgg gatcacgcgc tctgcatctt tggtttcttc cgacaggttg ctgatgccgt
    30001 caaagccggt aaaggaaaag cacaggattg tcgccccggt aatcatcgga ataacatgcg
    30061 catcgccaga ccagaacgga cgagtgctcg ccagcgtacc agcgccttca ccttcaaata
    30121 cgccataaac aaccatgccc agaatcaccg cgatcagcac tacctgcaac acgacgatta
    30181 cagtgttgaa gttcgctacg gatttcagac tacgcaggtt aaaggcggtc ataaaggcca
    30241 ccagcgccac cacaaacatc cacgatggga tggaaggcac cagagcttca aaatagattt
    30301 tcgccagcag aatgttgatc atcggcgcga acagatagtc gagcagagaa gaccaaccca
    30361 ccataaagcc gacagtcggg ctaatggatt tctgggcgta agtatatgca gagccagcag
    30421 aaggatagcg gcgaaccagc ttcccgtagc tcagagccgt aaacaggatc gcaatcaatg
    30481 cgaacgcata ggctgtcggc acatgaccat ccgtgaggcc tgaaacgata ccaaaagtat
    30541 caaacagcgt catcggctgc atataggcaa gacccatcat tacaaccgga actaacgtaa
    30601 gcgttttacg caattccacg cgagaggtgt ttggagtaac gttatgcgac atggtcattc
    30661 tcctttacgg tgataaccgt cgcgtaagca aaaaattgcc ccattttttt ggattcctca
    30721 gcgacaacaa ctgtcgattt ttagtaaata tctatccggt acgaagcccg gcctcttggt
    30781 atgaatgatt ggtttgaagc aaaaaataac cgacgctgat gaaacgtcgg tttttagtca
    30841 ttttttgaca gcggcgcatt gtgcctaaat gggggggaaa tgacaagaga atgagaagct
    30901 tgtcataata atttttcttt aaatggctga ttttccgtca tcagattttg cataaacacc
    30961 ggcgatcgca ctatttgcta aaatttcatc ccgccaccag ccgctggcaa gccctgcggt
    31021 ctgttcgttc cagccaatcc agacgggttc aaagagagtt tgggcgacca ctttcttctc
    31081 gattaacgcg ccactatcga gaaaacgttg caccagataa cggggtaaat agccgcagcc
    31141 gaggccgcta atttgcagct ccagcttggt tttaaaatcg aaaacggtaa tggcttcttg
    31201 ttcgtcgaga agttgtgagg ccgtagaagc gcctgcctgc gcagtgtccc ccaccacaat
    31261 cgcccgatat cgcttaatga tacgacggtt taacggctct tcttcctggg ctaacggatg
    31321 atgaggcgcg accgcaaaga cttgttccag atcgcccaac cgcgagaaac caaactcact
    31381 ggaagatggt ggctcatgca tcgcgccaac aataatatcc gctcgcccct gagtcagggc
    31441 atcccaggaa ccaccgagta cgccattgat aaacttcagg cgcgtcacgc tatgatgttg
    31501 ataaaaagct tcaatcagtg gcgcaagcag agaaaaagga aacgtgtcgt ccacgccaat
    31561 caccagctcg ttttcccagc cttcatgcag tttgatcgcc tgcttttcca gttctcgcac
    31621 ggtatgcaga acttcccgcc ccttctctaa taacattttt ccggtacggg tgaatttagc
    31681 gcggtgaccg ctacgatcaa gtaattgaat attaaggtcg ctttccagct tgtgaacggt
    31741 gtaactcaga gcggacggcg tcttgtagag tttagccgac gccgccgcaa aactgccttc
    31801 tttttctaag gcatcgagga tcatcaacac atccagcagt tgtttcatac acgccccctt
    31861 gcagtgcatg cggcgtcctg ctggcgtgat cgtttactcc agcggcatta ccagcggatc
    31921 gggatactgg tattcaaatc ccagttcatt acaaatccga ctgccatcaa taatcttacc
    31981 tttgccgctg tccagactat ttctgaactg cggaggttcc agccccagta aacgggccat
    32041 ctgcggatag aaaacattac gcgcagggtg agctggcgca catatattat agatgtgtcc
    32101 gcctttaggt gcctgtaaca acagagtgat agcgccaata acatcttcta aatggactaa
    32161 attaacacca tgttcaccat caggcgcggt ttttccggca aagaagcgtc cgggatgacg
    32221 tcccggtccg accagccccg caagacgcag aatatcgacc gaagtaccgg gtaaattgtg
    32281 cagccagtct tcgagttctt ctaatactcg tccactgttg gtcaccggat tacgcggggt
    32341 ggtttctttc accgtgcctt gcgcgtcgcc atagacagac gttgagctgg taaaaataat
    32401 gcgaggaata cgatgggcca gcgcgctatc cactaactct tgtaccgctt gtaaatagaa
    32461 ctcatcgccg gggccgctac gacgtgccgg aagcgtaatg accagcgcat cggcatccat
    32521 cagggcatcc agatcgtcag aatcgcaaac taactcaggc tccatgcgaa gcagatagct
    32581 atcaatgcca ctcattcggg ccgcttcgac accatcttgt gtggttttac tcccggtgac
    32641 ttgccagcct cgcgctgaaa gtgacatcgc cagcggcatg cctaaccacc ctaacccgac
    32701 aattgcgacc tttttcatcc gttatctcct gatacacctt agatctataa ggctacgcta
    32761 gcgtatcaaa actgacaatt cattctatga atgaatctgt tcaataatga taacgacatg
    32821 ctgcaatgag cagtgaatcg tcggtaaccg cgtataccag acggtgctcc tctgtaatgc
    32881 gtcgggacca gaaacctgac aaattatgtt tcaggggttc tggcttcccc ttaccttcaa
    32941 atggcgttct gcgggtatct ttgataagtt cattgatctt tttaacaatt cgcttatctg
    33001 tttcctgcca gtacagataa tcatcccatg attcctcaga ccagattagt ttcactcaat
    33061 aatgtccttt tctgttcctt tgcctgattt caggctatcg attgagtcca tcaatctccg
    33121 ggcgttagcg ggggagcgca gtagataagc cgtctcttcc agcgagttgt attcttcgag
    33181 tgacatcaga acacaagcct ctccattctg acgagtaata aggatcgggg catgatcttc
    33241 aacggctttc atcattgttg ccgacaaatt ctgacgcgct tcgctgtagc taattgtacg
    33301 catgtcaatc tcctcttttg tacagtttat tgtacaatga tgggcgttaa ttaactattt
    33361 attaattagt ttgtagatca aggtattgtc agtgagacga aaatccaggc ttcgctattt
    33421 ttggtgccgt cagcttagag gacagtcctc ttagccccct cctttccccg ctcattcatt
    33481 aaacaaatcc attgccataa aatatataaa aaagcccttg ctttctaacg tgaaagtggt
    33541 ttaggttaaa agacatcagt tgaataaaca ttcacagaga cttttatgac acgcgttcaa
    33601 tttaaacacc accatcatca ccatcatcct gactagtctt tcaggcgatg tgtgctggaa
    33661 gacattcaga tcttccagtg gtgcatgaac gcatgagaaa gcccccggaa gatcaccttc
    33721 cgggggcttt tttattgcgc ggttgataac ggttcagaca ggtttaaaga ggaataacaa
    33781 aatgacagac aacactcgtt tacgcatagc tatgcagaaa tccggccgtt taagtgatga
    33841 ctcacgcgaa ttactggcgc gctgtggcat taaaattaat cttcacaccc agcgcctgat
    33901 cgcgatggca gaaaacatgc cgattgatat tctgcgcgtg cgtgacgacg acattcccgg
    33961 tctggtaatg gatggcgtgg tagaccttgg gattatcggc gaaaacgtgc tggaagaaga
    34021 gctgcttaac cgccgcgccc agggtgaaga tccgcgctac tttaccttgc gtcgtctgga
    34081 tttcggcggc tgccgccttt cgctggcaac gccggttgat gaagcctggg acggcccgct
    34141 ctccttaaac ggtaaacgta tcgccacctc ttatcctcac ctgctcaagc gttatctcga
    34201 ccagaaaggc atctctttta aatcctgctt actgaacggt tctgttgaag tcgccccgcg
    34261 tgccggactg gcggatgcga tttgcgatct ggtttccacc ggtgccacgc tggaagcaaa
    34321 cggcctgcgc gaagtcgaag ttatctaccg ctcgaaagcc tgccttatcc agcgcgatgg
    34381 tgaaatggaa gaatccaaac agcagctgat cgacaaactg ctgacccgta ttcagggtgt
    34441 gatccaggcg cgcgaatcaa aatacatcat gatgcacgca ccgaccgaac gtctggatga
    34501 agtcatcgcc ctgctgccag gtgccgaacg cccaaccatt ctgccgctgg cgggtgacca
    34561 acaacgcgta gcgatgcaca tggtcagtag cgaaaccctg ttctgggaaa cgatggaaaa
    34621 actgaaagcg ctgggtgcca gttcaattct ggtcctgccg attgagaaga tgatggagtg
    34681 atcgccatga gctttaacac aatcattgac tggaatagct gtactgcgga gcaacaacgc
    34741 cagctgttaa tgcgcccggc gatctccgct tctgaaagca ttacccgcac tgttaacgat
    34801 attctcgata acgtgaaagc acgcggtgat gacgccctgc gggaatacag cgcgaagttt
    34861 gataaaacca cggttaacgc gctgaaggtg tctgctgagg agattgccgc cgctagcgaa
    34921 cgcctgagcg acgagctaaa acaggcgatg gcggtggcag taaaaaatat tgaaaccttc
    34981 cacactgcgc aaaaactgcc gccggtagat gtagaaacgc agccaggcgt gcgttgccag
    35041 caggtcacgc gcccggtcgc ttcagttggg ttgtatattc ctggcggctc cgccccgctc
    35101 ttctcaacgg tattaatgct ggcgactccg gcgcgtattg cgggctgtaa aaaagtggtg
    35161 ctgtgctcac cgccgccgat tgccgatgag atcctttatg cggcgcagct gtgcggtgtg
    35221 aaggacgtgt ttaacgtcgg cggcgcacag gccattgccg cgctggcgtt tggtacggaa
    35281 tctgtgccga aagtggacaa aatcttcggg ccgggtaacg cctttgtcac cgaagcgaaa
    35341 cgtcaggtga gccagcgtct ggacggtgcg gctatcgata tgcccgcagg cccgtcggaa
    35401 gtgttggtca ttgccgacag cggcgcaacg ccggatttcg tggcttctga tttgctttct
    35461 caggctgaac acggcccgga ctcacaggtg attttactga cgcctgacgc tgatatggcg
    35521 catcaagttg ccgaagccgt cgaacgccag ttagcagaac tgccgcgtgc cgaaaccgcc
    35581 cgccaggcac tgaacgccag ccgcctgatc gtgactaaag atttagcgca gtgcgtagag
    35641 atctccaacc agtacggccc ggagcacctg atcatccaga cccgcaacgc ccgcgatctg
    35701 gtcgatggca tcaccagcgc cggttcggta tttcttggtg actggtcacc ggaatcggca
    35761 ggtgattacg cctccggcac caaccacgtt ctgccgactt acggttacac cgccacctgt
    35821 tccagcctcg ggctggcaga tttccagaag cgtatgaccg tgcaggaact gtcgaaagaa
    35881 ggtttctccg cgctggcttc gaccattgaa acattggccg ccgccgagcg cctgaccgcc
    35941 cataaaaatg ccgttacttt gcgtgttaac gcccttaagg agcaagcatg agcaccgtga
    36001 ctattaccga tttagcgcgt gaaaacgtcc gcaacctgac gccgtatcag tcggcgcgtc
    36061 gtctgggcgg taatggcgac gtctggctga acgccaacga ataccccact gccgtggagt
    36121 ttcagcttac tcagcaaacg ctcaaccgct acccggaatg tcagccgaaa gcggtgattg
    36181 aaaattacgc gcaatatgca ggcgtaaaac cggaacaggt gctggtcagt cgtggcgcgg
    36241 acgaaggtat tgaactgctg attcgcgctt tttgcgaacc gggtaaagac gccatcctct
    36301 actgcccgcc aacgtacggc atgtacagcg tcagcgccga aacgattggc gtcgagtgcc
    36361 gcacagtgcc gacgcaggac aactggcaac tggacttaca gggcatttcc gacaagctgg
    36421 acggcgtaaa agtggtctat gtttgcagcc ccaacaaccc gacagggcaa ctgatcaatc
    36481 cgcaggattt tcgcactctg ctggagctaa cccgcggaaa agccattgtg gttgccgatg
    36541 aagcctatat cgagttttgc ccgcaggcat cgctggctgg ctggctgacg gaatatccgc
    36601 acctggctat tttgcgcaca ctgtcgaaag cttttgctct ggcgggcctt cgttgcggat
    36661 ttacgctggc aaacgaagaa gtcatcaacc tgctgatgaa agtgatcgcc ccctacccgc
    36721 tctcgacgcc ggttgccgac attgcggccc aggcgttaag cccgcaggga atcgtcgcta
    36781 tgcgcgaacg agtgacgcaa attattgcag aacgcgaata cctgattgcc gcactgaaag
    36841 agatcccctg cgtagagcag gttttcgact ccgcaaccaa ctacattctg gcgcgcttta
    36901 aagcctccag cgcagtgttt aaatctttgt gggatcaggg cattatctta cgtgatcaga
    36961 ataaacaacc ctctttaagc ggctgcctgc gaattaccgt cggaacccgt gaagaaagcc
    37021 agcgcgtcat tgacgcctta cgtgcggagc aagtttgatg agtcagaagt atctttttat
    37081 cgatcgcgat ggaaccctga ttagcgaacc gccgagtgat tttcaggtgg accgttttga
    37141 caaactcgcc tttgaaccag gcgtgatccc ggaactgctg aagctgcaaa aagcgggcta
    37201 caaactggta atgatcacca atcaggatgg tctgggaaca caaagtttcc cgcaggcgga
    37261 tttcgatggc ccgcacaacc tgatgatgca gatcttcacc tcgcaaggcg tacagtttga
    37321 tgaagtgctg atttgtccgc acctgcccgc cgatgagtgc gactgccgta agccgaaagt
    37381 aaaactggtg gaacgttatc tggctgagca agcgatggat cgcgctaaca gttatgtgat
    37441 tggcgatcgc gcgaccgaca ttcaactggc ggaaaacatg ggcattaatg gtttacgcta
    37501 cgaccgcgaa atcctgaact ggccaatgat tggcgagcag ctcactaaac gagaccgtta
    37561 cgctcacgta gtgcgcaaca ccaaagagac acagattgac gttcaggtgt ggctggatcg
    37621 cgaaggtggc agcaagatta acaccggtgt tggcttcttt gatcacatgc tggatcagat
    37681 cgccacccac ggtggtttcc gtatggaaat caacgtcaaa ggcgacctct atatcgacga
    37741 tcaccacacc gtcgaagata ccggcctggc gctgggcgaa gcgttaaaaa tggctcttgg
    37801 cgacaaacgc ggtatttgcc gctttggttt tgtgctaccg atggacgaat gccttgcccg
    37861 ctgcgcgctg gatatctctg gtcgcccgca cctggaatac aaagccgagt ttacctacca
    37921 gcgcgtgggc gatctcagca ccgagatgat cgagcacttc ttccgttcgc tctcatacac
    37981 catgggcgtg acgctacacc tgaaaaccaa aggtaaaaac gatcaccacc gtgtagagag
    38041 cctgttcaaa gcctttggtc gcaccctgcg ccaagccatc cgcgtggaag gcgacaccct
    38101 gccctcgtcg aaaggagtgc tgtaatgaac gtggtgatcc ttgataccgg ctgcgccaat
    38161 ctgaactcgg tgaagtcagc cattgcgcgc cacggttatg aaccaaaagt cagccgtgac
    38221 ccggacgtgg tgttgctggc cgataaacta tttttacccg gcgttggcac tgcgcaagca
    38281 gcgatggatc aggtgcgtga gcgcgagctg tttgatctca tcaaagcctg tacccaaccg
    38341 gtactgggca tctgcttagg gatgcaactg ctgggtcggc gcagcgaaga gagcaacggc
    38401 gtcgacttgc tgggcatcat tgacgaagac gtgccgaaaa tgaccgactt tggtctgccg
    38461 ctgccgcata tgggctggaa tcgcgtttac ccgcaggcag gcaaccgcct gtttcagggg
    38521 attgaagacg gcgcgtactt ttactttgtt cacagttacg caatgccggt caatccgtgg
    38581 accattgccc aatgtaatta cggcgaaccg ttcaccgcgg cggtacaaaa agataacttc
    38641 tacggcgtgc agttccaccc ggagcgttct ggtgccgctg gcgctaagtt gctgaaaaac
    38701 ttcctggaga tgtgatgatt attccggcat tagatttaat cgacggcacc gtggtgcgtc
    38761 tccatcaggg cgattacggc aaacaacgcg attacggtaa cgacccgctg ccgcgcttac
    38821 aggattacgc cgcgcagggt gccgaagtgc tgcatctggt ggatctgacc ggggcaaaag
    38881 atccggcaaa acgtcaaatc ccgctgatta aaaccctggt cgcgggcgtt aacgttccgg
    38941 tgcaggttgg cggcggtgtg cgtagcgaag aagacgtggc ggcgttactg gaagcgggcg
    39001 ttgcgcgcgt ggtggttggc tccaccgcgg tgaaatcacc agaaagggtg aaaggctggt
    39061 ttgaacgttt cggtgccgat gccttagtgc tggcgctgga tgtccgtatt gacgagcaag
    39121 gcaacaagca ggtggcggtc agcggctggc aagagaactc gggcgtttca ctggaacaac
    39181 tggtggaaac ctatctgcct gtcggcctga aacatgtgct gtgtaccgat atctcgcgcg
    39241 acggcacgct ggcaggctct aacgtctctt tatatgaaga agtgtgcgcc agatacccgc
    39301 aggtggcatt tcagtcctcc ggcggtattg gcgacattaa cgatgttgct gccctgcgag
    39361 gcactggcgt gcgcggcgta atagttggtc gtgcattact ggaaggtaaa ttcaccgtga
    39421 aggaggccat cgcatgctgg caaaacgcat aatcccatgt ctcgacgttc gtgatggtca
    39481 ggtggtgaaa ggcgtacagt ttcgcaacca tgaaattatt ggcgatattg taccgctggc
    39541 aaaacgctac gccgaagaag gtgcagacga actggtgttc tacgatatca ccgcttccag
    39601 cgatggccgt gtggtagata aaagctgggt atctcgcgtg gcggaagtga tcgacattcc
    39661 gttctgcgtg gcgggtggga ttaagtctct ggaagatgcc gcgaaaattc tttcctttgg
    39721 cgcggataaa atttccatca actcccctgc gctggcagac ccaaccttaa ttacccgcct
    39781 ggcggatcgc tttggcgtgc aatgcattgt ggtcggcatt gatacctggt acgacggcga
    39841 aaccggtaaa tatcatgtga atcaatatac cggcgatgaa agccgcaccc gcgtcactca
    39901 gtgggaaaca ctcgactggg tacaggaagt gcaaaaacgt ggagccggag aaatcgtcct
    39961 caacatgatg aatcaggacg gcgtgcgtaa cggttacgat ctcgaacaac tgaaaaaagt
    40021 gcgtgaagtt tgccacgtcc cgctaattgc ctccggtggc gcgggcacca tggaacactt
    40081 cctcgaagcc ttccgcgatg ccgacgttga cggcgcgctg gctgcttccg tattccacaa
    40141 acaaataatc aatattggtg aattaaaagc gtacctggca acacagggcg tggagatcag
    40201 gatatgttaa cagaacaaca acgccgcgaa ctggactggg aaaaaaccga cggactaatg
    40261 ccggtaattg tgcaacacgc ggtatccggc gaagtgttaa tgctgggcta tatgaacccg
    40321 gaagccttag acaaaaccat cgaaagcggc aaagtcacct tcttctcgcg cactaaacag
    40381 cgactgtgga ccaaaggcga aacgtcgggc aatttcctca acgtagtaaa tattgcgccg
    40441 gactgcgaca acgacacgct actggtactg gcgaatccta tcggaccaac ctgccacaaa
    40501 ggcaccagca gctgcttcgg cgacaccgct caccaatggc tgttcctgta tcaactggaa
    40561 caactgctcg ccgaacgcaa atctgccgac ccggaaacct cctataccgc caaactgtat
    40621 gccagcggca ccaaacgcat tgcgcagaaa gtgggcgaag aaggcgtaga aaccgcactg
    40681 gcagcgacgg tacatgaccg ctttgagctg accaacgagg catcggactt gatgtatcac
    40741 ctgctggtgc tgttgcagga tcaggatctg gatttaacga cggtaattga gaacctgcgt
    40801 aaacggcatc agtgagttgc agggtaagcg gatgcgatgt tgttgtcgca tccggcaaaa
    40861 aaaaagggca aggtgtcacc accctgcccg ttttctttaa agctgcaaag attacttcgc
    40921 gttataatta cgcagagcgt tacgccccag cacaatcccc gcgccaacca tgccgcccag
    40981 cagcaccgcc agaatcaagg taattgcctt tttcgggcta tcgcgacgaa taggtaacgt
    41041 cggtttcatc acataacggt aagcatgaat atcaagatca tcaaccttta agttttcaat
    41101 atccaacagg ttttgacgtg tctgatagta gtttggtgag aacaccaacg gacgggtcgc
    41161 ctcatgctta atcatcgact ccagcgcttc gctccccaaa aggaacaacg tatcttgtgt
    41221 cacatcttca gtctgctgaa cctgtggctt tgccacctgc gcctgattcg catactgcaa
    41281 cgcttcctga atctgacgga tacgcagatc tttctgctcc tgcgcgacta cttcctgcgt
    41341 tcttaaagag tcctgcaagt ttttccgtcc cagagcaatg ttgtctttga gatccttttc
    41401 tagctcttga ttcactttat catcaacttg ctgaatgtat tgggccaact tcatttgtgc
    41461 tccctctgca gtttgcccaa cataagaaac agtcaatggt aattgctggt tcttaacaga
    41521 aggttcgatg gtaagttttt ctggttcttc ctgattatcc agcgtttctg ctaatgcaga
    41581 gaaggcagaa ctgaagcgac caattaacgt ctcctgtaaa tccgatactt tcggtgcagc
    41641 ctgaccatag ataacattca tggcattgtt atagccagca atttgcccca cgtcgggctg
    41701 agtgataatt gctgttgacg tccatttctc cttcgctact gccaaatatc caatagccag
    41761 ggcaatagcc acaatgacgg aaatgatgat tgtcatcttg ccacgccaca actgcactag
    41821 taaatcaatc aaatcaatct gttccgggtc atggttttgc ccagaaacat tattattttc
    41881 tactctcatc attaccctaa ctgacggaaa tttccaagtc cgcatagttt agcgataatc
    41941 ctagtgaatg ctataggata agtgataaaa ataatgaatt attggggagg taacaaaaga
    42001 caatcagggc gtaaatagcc ctgataacaa gatgttagtc gctgccaaag agatcgcggg
    42061 tgtatacctt atctgccaca tctttaagct cttctgccat tcggttagag ataatgacgt
    42121 cggcttgttg tttgaagatg gcgagatcac gttccagccg agagttgaag aatgagtctt
    42181 ctttcatcac tggctcgtag atgatcactt caacgccttt cgccttgata cgcttcataa
    42241 tcccctgaat agaagacgca cggaagttat ctgaaccgct cttcataatc agacgataaa
    42301 tacccaccac ttgcggtttg cgtgacaaaa tggcatcggc aataaaatcc ttacgcgtgc
    42361 ggtttgcatc gacaattgcc gagatcaggt tattcggcac agactgatag ttcgccagta
    42421 actgcttggt atctttcggc aagcaataac caccataacc aaacgacggg ttgttgtaat
    42481 ggttgccaat acgcggatcg agacaaacgc cttcgataat ctggcgagta ttcagaccta
    42541 aactttctgc gtagctatcc agttcgttaa agtatgccac gcgcatcgct aggtaggtgt
    42601 ttgcaaaaag tttaatcgct tctgcttcgg tcgaatcggt gaacagggtc gggatatttt
    42661 gcttaatcgc gccttcctgt aacaacgcag caaaacgttc tgcgcgttct gaacgctcac
    42721 cgatgacaat acgtgaagga tggagattat cgtaaagggc tttaccttca cggagaaatt
    42781 ccggagagaa aataatattt tcagtacgat atttcttatg catcgcttcg gtaaaaccaa
    42841 cgggaaccgt tgatttgatg accataaccg cataaggatt tatctcaact acgtctttaa
    42901 ttactgattc tacactggat gtattgaaat aattagtttt aggatcatag tcggttggag
    42961 tggcgatgat gacataatca gcatcccggt aagcttcatt tttatctaat gtggcattaa
    43021 agtgtatttt atctgattgc aaaaactgct gaatttcctt atcaacaata ggagatatcc
    43081 gatcattcag catagcaacg cgtgacggta aaatatctaa tgccacaacc tcatgatttt
    43141 gtgcgattag aagcccgttt gacaagccta catagccagt accggaaatg gtgattttca
    43201 tttcatgctc tcagaattaa cttaactgtg aatcatgatg tttttagcat cctgataaga
    43261 gctaaatgtt ttaacgctac gttattagct ggtcaacgct acgaaaaaca attcagattt
    43321 tctttataag aatagctgat atttattaag ttaatattaa gcagtgaaat ttagtctata
    43381 agataaatgg caaaaaaaaa gcccggtgca atatacgccg ggcctcaatt ttactgttgg
    43441 ttaaatcaga ttaatccagc cattcagtat ggaacacacc ttctttatca atgcgcttat
    43501 aagtatgtgc accgaaatag tcacgctgtg cctggatcag gttcgcaggc agaacagcgg
    43561 cacggtagct atcgtaatag gcaaccgcag cggcgaaggt cggaaccggg ataccgttct
    43621 gtactgcata agcaacgaca tcacgcagcg cctgctggta gtcatcggca atttgcttga
    43681 agtacggagc cagcagcagg ttagcgatct gcggattttc ggcatatgca tcggtgattt
    43741 tctgcaggaa ctgcgcacgg atgatgcagc cagcacggaa aatcttcgcg atttcgccgt
    43801 agttcagatc ccagttgtat tcttcagacg cagcacgcag ctgagagaag ccctgagcgt
    43861 aagaaacgat tttgcccaga tacagcgcac ggcgaacttt ttcgatgaac tcagccttgt
    43921 cgcctgctga ctgagcttgc ggaccagaga gaactttaga tgcggcaacg cgctgatctt
    43981 tcagagaaga gatataacgt gcaaacacag actcggtaat cagcgacagc ggttctccga
    44041 gatccagcgc gctctggctg gtccatttac cggtaccttt gttagccgct tcatccagga
    44101 tcacatcaac caggtagtta ccgtcttcat cttttttagt gaagatatct ttggtgatgt
    44161 cgatcaggta gctgctcagt tcaccgttat tccactcggt aaaggtctgt gccagttctt
    44221 cgttagagag attcaggccg cctttcagga gagaataggc ttcagcaatc agttgcatat
    44281 caccgtattc aataccgttg tgaaccatct tcacataatg gcctgcgcca tcagcaccga
    44341 tataggtaac gcacggttcg ccatcttcag caacggcggc gattttggtc aggatcggcg
    44401 caaccagttc ataggcttct ttctggccac caggcatgat ggacggccct ttcagcgccc
    44461 cctcttctcc gccggaaaca ccggtaccga tgaagttaaa gccttcggca gaaagctcac
    44521 ggttacgacg aatggtgtcc tggaagaagg tgttaccacc atcgatgatg atgtcgcctt
    44581 tatcgagata tggtttgagc gaatcaatag cagcatccgt gcctgcacct gctttcacca
    44641 ttaacaggat gcgacgaggc gtttccagag attcaacaaa ctctttcacc gtatagtaag
    44701 gaaccagttt cttgcctgga ttttcggcaa tcacttcttc cgtcttttca cgggaacggt
    44761 tgaaaataga gacggtataa ccacggcttt cgatgttgag cgcaaggttg cgccccatca
    44821 ctgccatacc gactacgccg atctgttgct ttgacattgt ttactcctgt cagggggtgg
    44881 tcaccgccga gtgtgcggct taaatgtggt atagatgtta actcactcat agagatggtg
    44941 aatagcatta cctttgctat accacactga atatctcacc ttttccgtct tttaaaactt
    45001 atttatatga aattactgaa ttacttgttc agtaactcaa gaataagttt tgtcttttct
    45061 tccatcagcg gcacatcacc gcgagattcc acattcaacc gcaccaccgg ttcggtgttg
    45121 gaggtgcgca ggttaaagcg ccagtcggca aaggtcatgc tgatgccatc ggtgcgatcc
    45181 accgccagcg cctcgcggct aaaatgctgt tccacgcggt taatcgcctc aaagggttgc
    45241 gccagtttgc tgttgatctc accgcttgcc ggaaacgctg ccatccggtc gcgcaccagt
    45301 tcgcccagcg tttttccttt caggcacacc agttcggcga ccagcagcca cgggatcatg
    45361 ccgctgtcgc agtaagcgaa atcacggaag taatggtggg cgctcatttc accaccgtag
    45421 atggcgtctt ccttgcgcat ccgttcttta ataaaggcgt gtccggtttt cgacatcacc
    45481 ggcgtgccgc ctgcggcagt caccacatca acggtgttcc aggagagacg cggatcgtgg
    45541 atgatcttcg cgccgggatt tttttcgagg aatgcttctg ccagcaggcc gacaatgtag
    45601 tagccctcaa taaactgccc tttttcgtca aacaggaagc agcggtcaaa atcgccatca
    45661 aaagcaatgc ccatatccgc accgtgtttg atgaccgcat tgcgggtgtc gtcgcggcat
    45721 tccggcagta gcgggttagg aataccgttg gggaaattgc cgtccggcgt gttgtgcact
    45781 ttgattaatt ccacgggtgc gccaagggct ttaaagcggg cttcgatggc gtccaccacc
    45841 ggacccgctg cgccattccc ggagttaatc accagcttga gcggcgtgag gtttttgaca
    45901 ttgatataac cgaacaggtg atcaacgtaa gcgtcacgca gattgatttg ctgatagcga
    45961 ccgcgtttgg tttcatcaac gggaggaaag tcgttggctt ctgccagacg ctggacatcg
    46021 cgcagtccgg tatcaccgct gatcgggcga gccccttcgc gcaccagttt catgccgttg
    46081 taatccatcg ggttatggct ggcggtaact tcaatgccgc catccacgcc gagatggaac
    46141 gtggcgaaat agatctcttc ggtgccggac atgccaatat ccagcacatc gacgcccgca
    46201 tcctgtaaac ctctcgccag cgccagtttt aaggtttcgc tggtgaggcg gacatcaccg
    46261 cctaacacaa tggttttcgg tttgagaaat tcgccatagg cgcgaccaat gcgccaggcg
    46321 atatcttcat tcagttcttc gcctaatttc ccgcgaatat cataggcttt aaagcaggtt
    46381 aattttttca tgtcgttacc ctttttcagg caatagttgg ccctgaccga aacggccaat
    46441 tttgtttttg tcgttattta catctcggtt tcgcattcat tgcctgatgc gacgtttaca
    46501 cccgtccgta gcgatccgcg aaacgcacca catcatcctc ttcgagataa gagccggagc
    46561 gcacttcaat taaatcgagc ggaattttcc ccgggttttc caggcaatgc atcgccccca
    46621 gcggaatata aatggactcg ttttcaccaa gcagtttgat atcaccatca atggtgactt
    46681 ttgccgttcc cgcgacaacc acccagtgtt ccgcgcggtg atggtgcatc tgtaccgaca
    46741 agccctcgcc cggtttcacg gtgatgcgtt tcacctggta gcggtcgccc gcgtcgatag
    46801 agtcatattt gccccacggg cgatagactt cgcgatgcac ccgatgctca tggcgaccat
    46861 cggctttgat ctgctcgacc acttttttca catcctgcac cgcgttacgg tcggcaatca
    46921 gcaccgcatc tttggtctgc accactacca gatctttcac cccgacggtg gttacgaggc
    46981 cagattcggc gtacacatag ctgttttcag ttttgtgatt aatcacatcg ccgtggcaaa
    47041 cgttgccctc ggcggtgtgg gcgctaattt cccataatga ggaccaggag ccgacatcgc
    47101 tccagcccgc atccatcggc accacaacgg catctgccgt acgttccatg accgcgtaat
    47161 ccaccgactc ttccgggcag gcgagaaacg cttcttcatc cacacgaata aaatcgagat
    47221 ccggatcgac ggcgctcatc gctttttcac aggcatcgag aatatccgga cgatattttt
    47281 tcagttcttc gagatagcgt ccggcgcgga acaggaacat gccgctgttc cagtaatatt
    47341 cgccgcttgc cacataggcc tgggcggttt ccagattcgg tttttcgaca aactgcgcca
    47401 cttcaaaggc caccgtatcc tgctcacccg cagacacttc accgcgacga atatagccat
    47461 aaccggtttc tggtagatcc ggcacaatgc cgaaggtcac cagcttgccc gcttcggcat
    47521 atggcatggc attacgcacg gcggcacgga acgcgtcttc atcggcaatc acatgatccg
    47581 ccgccaatac cagcattaac gggtcgctct ccgggctatg acgttttgcc gccagcgccg
    47641 ccagcgcaat ggcaggtgcc gtgtttcgcc ctgccggttc gagaataatg ttctcggtaa
    47701 gtttgttcag ttgacgcagc tgttccgcga caataaagcg gtgctgctca ttgcaaatca
    47761 ccaccgggct ttcgcactcc acgccgttca ggcggcagat ggtggtttgc agcatggtga
    47821 gatcgccttt caggcataaa aactgcttgg ggtaaagtac gcgggaaagc ggccataagc
    47881 ggctaccgga gccacctgcc atcacaactg gatagagttt cgactgcgcc ataattatcc
    47941 ccgaatatca tttataaatt gacgtaacac gttctcttta tcgagtgtgc gttcggcata
    48001 ttcacgtgcc accgtgttgt gtttgggcag caggagcgcc tgacggatcc ccgccaccag
    48061 cgcctcgacc gattccggtt ctacgcaaac cgcaatgccc ggaaaggttt cgcaaagctg
    48121 ccccagttct gtgtgggcct ctgcggtaat caccgcgtta ccgcctaccg ccagaatatt
    48181 ggtcagtttc gacggcaata cggcatctgc cgcgccgcgt ttttgcacca ccagatggca
    48241 atcgcccatc ttcagcagtg cgggtaaagc gtcatacgat tgcagcggga aaaattgcat
    48301 gttgcgcagt ccacgctgct gcgccatttt ttccagccgc gctttgccgc cgccctgccc
    48361 gacaatggca aaaatcagcg gttcatcgcg cagacgatcg gcagcttcaa taacgttttc
    48421 cagcccctgc ttttcaccaa tattgccgga gtaaagaatg atttttttgt tatccggcag
    48481 gtcaagctgg ttacgaaggg cgtcaacatc ggcatctgca acatgctgaa aacgggcaat
    48541 ttccgaccag ttggggaaga agatgacgtt ttccgccgcc acgccttttt cgatggcttt
    48601 attcatcatc gaacgcgaaa tcgtggagac gttatcgacg ttatgcagtc cgctacgttc
    48661 gaacgccgtt gccagctgtg ccactttgct gcctttgcct tttccggcaa ggcccagccc
    48721 cagcatggcg tccacttcgt aatcctgaat atgcagcacg gtacgcgcac cagaaagttt
    48781 cgccagcagg cgcattcccg gcgtgcaaaa cagcgttggc accacgccga taatgcgatc
    48841 cggcttccag cgacgttgcg ccatcagagg aaagaaactg ctgacggcaa aactgccgag
    48901 atgtaacagg cgtttcaggg tgctcggctg ttttggcacg tacagcgggc agcgccacac
    48961 cgtggcggcc ccctcttctc gtttgtaccg ccaggcggaa tagttctcgc ccacctgcca
    49021 ctgcgggtag taaggcggtg cggtaataac ccgcacctca tgaccttgtg ccgccagcca
    49081 ttccaccatc tcgccggtgt atttgccgat accggttaac tccggcgagt agttaatgcc
    49141 gtagaccagt attttcataa tccgggtact ccggcacgct tctcagcgag aaaataggcg
    49201 cggctgttag cgtgaacatc gttactggcg agcagcgcgt ccggcgtcag ccagcggtaa
    49261 tcgtcatgct gctcatccgg cagtatcagc tcttcttcag cgactctgaa gcgaaaaccg
    49321 agcaccacat agtgagtggt gaaatccgtg ccggagaagt tatcgtcata aaagtgctgc
    49381 cagacaccgt aaaactggcc tgctgttatc ggcagacgca gccccagttc cgccatcgtc
    49441 agccgctcga atgcggcttc cagcgtttcg tctttctgca cgcgccctcc cggcacaaac
    49501 cagtaaccct gcgccgggcg gttggttctt ttgccaagca gaaactcacc gcgactgttc
    49561 tcgacaataa agtcgagaga gacaagcggg gtggagcgca ctaccgtggc aaagtcttcc
    49621 tgacgtaaaa acatcattac ccccgaaagc ggtcttgatt ctcaaggaac cactggtaag
    49681 tgctggcaag ccccgcttcc agtgagattt cgtgatacca gccaagctga tgcaggcgcg
    49741 tcacatccag cagtttgcgc ggcgtaccat ccggtttgct ggcatcaaaa accacccgac
    49801 ctttgtaacc caccactttg gcgatggttt gcgccagctc gcggatagtg cagtcaacgc
    49861 ccgtgccgac gttaatgtgc gacagcatcg gctgggtgtt ctccagccag acttcgtgcg
    49921 ccagctccat gacatgaatg ctcgccgccg ccatatcatc gacgtgcagg aattcacgca
    49981 tcggtgtacc gctgccccat accaccacgt ccggtgcatt ctgtgccgtc gcctcgtgga
    50041 agcgacgcag caatgcaggg atcacatgcg aattactcgg gtggaagttg tcatgcgggc
    50101 catacaggtt ggtcggcatg actgagcggt aatcgcgtcc gtactggcgg ttgtatgatt
    50161 cgcacagttt gatcccggcg attttggcaa tggcgtaagg ctcgttggtc ggctccagcg
    50221 tgccctgcaa taactcgctt tctgccatcg gctgttttgc cagtttcggg tagatacagg
    50281 acgatccaag aaacagcagt ttgttcacgt cgttctgatg cgcggcgtga atgatgttgc
    50341 tctcaatcat catgttctgg tagatgaaat ccgccggata ggtgttgttg gcaacaatgc
    50401 cgcccacttt cgccgccgcc agatagacct ggtcaatgcg ttcgctggca aagaaatcat
    50461 gcaccgcgcg gctgtccagc aggttcagct cgtcgcgggt gcgtaatacc agttccacat
    50521 caccgcgctg ttcgagctgc cgcctgatgg cagaaccgac catcccacga tggccagcga
    50581 taaaaatgcg ttgtttactc atgcttatga ctccagcgcg atcgccacgt cgtagccgtg
    50641 agatttcagc agagagtgtt ttttcgccgc ttcgaggtca ttagccacca tttcagacac
    50701 catctctctg agggtgattt ccggtttcca gcccagtttt tcgtgcgctt tggtcgggtc
    50761 gccgagcagc gtttcaactt cagccggacg gaagtaacgc ggatcaacgg cgataatcac
    50821 atcacccggt ttaacgcccg gcgcgtcatg cccggtgacg gaaaccacaa tgcccttctc
    50881 ttcaacaccc gtgccttcaa agcgcagttt gatgcccagc tgtgccgctg ccatttccac
    50941 gaactgacgc acggagtact gaacgccggt cgcgataacg aaatcttccg gctgttcctg
    51001 ttgcagcatc atccactgca tttttacgta gtctttggcg tggccccagt cacgcaggga
    51061 atccatattg ccgaggtaca ggcacgactc cagcccctgg gcgatgttgg cgattgcgcg
    51121 ggtgattttg cgggtaacga aggtttcgcc gcggcgcggg gattcatggt tgaagagaat
    51181 tccgttacaa gcgtacatgc cgtaggattc gcggtagtta acggtgatcc agtaggcgta
    51241 cagtttggcg accgcatacg gagatcgcgg gtagaacggc gtggtttctt tctgcggaat
    51301 ttcctgcacc agaccataca gttcagaggt ggaagcctga tagaaacgag ttttcttttc
    51361 cagaccgagg aagcggatcg cctccagcag gcgcagcgta cccatcgcgt cgacgtcagc
    51421 ggtatattct ggtgactcaa aagagaccgc aacgtggctc attgcgccca ggttgtacac
    51481 ttcatccggc tgtacttcac gcaaaatgcg cgtcaggtta gaggtatcac tcaggtcgcc
    51541 ataatgcaga tggaatttcg ggttgcaggt gtgcggatcc tgataaatgt gatccacgcg
    51601 ctcggtgttg aatgacgatg cgcgacgctt aataccatgc acctcgtaac ctttttccag
    51661 cagaaactct gccaggtaag aaccgtcttg tccggttaca ccggtgatga gagcgacttt
    51721 tgacatgtct tattcctctg tatttttgaa tttattcagt ttcaacgcgt tcgcgtatca
    51781 ccactgcggg attcccccgg caaaccacat ttgccggaag cgatttaaaa acactgcttc
    51841 gtgcacccac gacggtgccg tcgccgattg tgacgcctgg ggcaacaaag acatcggttg
    51901 ccagccagca tttctcgcca atcacaatag gcgtggcgtt aatggtgaaa tgttgacttg
    51961 catggtcgtg gctaccggtg cataaataac ttttttgcga tatcaccgaa tgtgcgccaa
    52021 tggttatttc accgagggta tataaattga cgtcatcgcc gacccacgcg taatcaccta
    52081 aggttaattt ccacggatag gtaattttta ctgacggacg aataactacg ttttttccta
    52141 tttttgctcc gaataaacgt aataaaaaag cccgccagcg atacaatact tgtggcgacc
    52201 aggcaaatat tgttgcctgt actgcccacc ataattgcac tttaatagcg ttgccgcccc
    52261 ggaacccttt cggcaccgag aaaccgctta aatcttgcat tggttttcct tatattcaga
    52321 ctttgttata taaggctttc gtctttgagg tagtacgttg gcgtaaatgc caggataatt
    52381 cagcccaaaa gccaggcaca tgtaatattt gtcgttggac ttttttcgcg tcagcacaca
    52441 attccatatt attggtggta gataccccac ccatggaaaa ttcagacacc aggccattga
    52501 gttttttaaa tgcataaccg gctttataca ttttggctgc cagcgcgtag tcggaagaaa
    52561 ctttatattc caggtcataa cgccattttt tcaagccgga tacagggaaa aatatcgcct
    52621 gatgactggc gggcaggctg tgataaatat accagcccgg tttggcgcta cgtttaattt
    52681 tatgcccgtc gccaaaatcc agcagcgcat cgccggtgat catcatgtta tctttttgca
    52741 tttttaactt acggacaaaa tttgcggcat cctgatgaaa aatatcgccc gaattgagaa
    52801 acaacgcgaa cttgccttgt gccatcgtaa tgcctttgtt catggcgtcg tagataccgt
    52861 tatctggctc gctgacaaag cgtaggttat agataccatt gagattttcc agatactcac
    52921 gggtgccgtc attggaaccg ccatcgacaa caatccattc gaagctgata tcttccgcct
    52981 gcgccagatg cgccagcgag gcatgtgttt tgactatccc ttcgaggtta cgaaacgcga
    53041 cagtgattat gctaagcaac atgttcttat tcttacctcg taatatttaa cgcttttcgc
    53101 aaaataaacg gacagacgat taaaaatgca tattccgggc taaatatcga accggtaaaa
    53161 aacagcgata ccggagtaaa aagatataat tgcacgcgaa aattgcggtt atcaccaaaa
    53221 gcgttgatca tcatttttat cactttcccc atgtaccaca gcgataaaaa cactgcgaac
    53281 caggaaaaat aaataatcag cagatacaaa ccattgtcta tggtttttcc gacatccgca
    53341 ccgttaaata ttccgaatga tgcgacgtat tcataaagtg agccaaatct gactacaccg
    53401 tcaatatggg tcaaagaata accgaccatc accagcggac cgacaatacg ataatacgat
    53461 gacgatcctt ccgtacctaa atcgcccaga cgggtggaaa tataaggaaa cgcgattacc
    53521 acaccaaccc ggaatacagc cagagaaatc aatgccaacg gtaacttttt cttaatcgcc
    53581 tctttattca gatattgaaa tgcccactcc agcaaataaa acaggataaa agtcataacc
    53641 cctgaaaacg atccggataa tattatccct gcgagaatca tagcatcggt tttaggcgtt
    53701 ttgataccaa actgtttgat gctgagccaa attgagatta atgccagagc gaaaaatgcc
    53761 ggttcgaaat aaagtgctgt cgtgcgcttg ccgccgaatt taatgaaatt cagtacatag
    53821 ctgttgctgt aaatcagata tttcgaaatc gactccataa tactgctgcc accggtgagg
    53881 ataatttgcg ccatctccac cgccgccagc gccaccacca gcccgatcac cagataaaag
    53941 aaacgtaata tcttgcgatg gttgtgtggc gaaatcgttt taaagcgaat actccacacc
    54001 atgccaataa tgatcacaat atagacaaac agcatggttg aagtgacgta tttactggca
    54061 tccagcgact gaccaaacag atagttaaac gccgtgagtc ccgcaccaat ccctaaggca
    54121 atcatcaatt ttttaacgct gatgcgttct aaaaacagca gtaacaagac aggtaaaaaa
    54181 gtgacgatgg tgatggggaa actttcgcca agctgggcga ttttgacgtt aaccagcagg
    54241 tagatcagcg gcagcagcag gtagctacag attctgatag aagttgacat actcctccag
    54301 catctgttgt ccactgtagg tggcgcggct gcgttggctg aactcagcca gcgtggtacc
    54361 aaatatcgcc tgcgcgattt ccggtttgct taactgcacc agttgcagca cgtcttcttc
    54421 gtcgacggtt ttaccgccgg atttttgcaa cacttcccgt gccgcgtcgc tatgggtggc
    54481 aatcaccggc acgccaattg ataacgcctc acacaaaatc agcgggtagt tatcgacgcg
    54541 agaactgaat accagcgcat ccatctgatt gagcgcgctc atcaacttgc gcttgtcagt
    54601 ttcaaagccg tgattaacca cgttgccagc ggtgaacggc gagaacttac caaaggtatg
    54661 cagttcgatt ttgtcgccca gcgccatcat ctcacgcacc agttgctggt tagtcttgcc
    54721 gtcgtaacgc aagtcatgcg ccaccaccgc gattttcggc ttgccctggg tttcgcgcac
    54781 cggaggcaag tccgccagaa tcgcttcggt tgccatatca ataccattat tgataatccg
    54841 gcaacgccct ggaccgtaca ggctattgaa agcgtcagcc acatgctggc tgggggaaat
    54901 aaactgacag cccagcgcca gcatctcacg gaataactgg cgtttgcccg ccaccagctg
    54961 gtgtgcgcga tcaatcttca ccggcggata gttatttaag gtcgggcatt tctggcagcc
    55021 cgttttccag ccttcgcaac cgtcggtaaa ggcgcagcgt ccggtaacac tccagtggtc
    55081 gtgcagcgtc cagaccagag tgacgtccgg tttatggttt ttcacttttt cgcaaaagcg
    55141 caccacgctc ttaagattta gccagtagct gtgcagcaca tgaaaatgca ggaccaccgg
    55201 acccggtgtg cgagtaatgg tgcgatataa ctcattgaaa ttgccaaaca gatcgcgatt
    55261 aaacagacga aacagggcaa tattcgccat cgcggtcatc cgcggcgtat gtttgatgac
    55321 ctgcggatag ttctgatggc tgacgctctc tttgccgcct ttgccataac cgtagacaaa
    55381 atgtgacgcc agcccctgtt gcagcgcacg ctggtggaga tctaacgcca cacctgctgc
    55441 cccgccttcc gccagtcgca cattaaattg cagaatattc atttaattac cttcactcgc
    55501 gctttttctc ccaccaccag cgcgttgtcc gggacagagt cgagcaccac gctgcccgca
    55561 cccacggtga cgttgttacc aagcgtgata tcaccaagaa taatgacgtt ggcaccgagt
    55621 tcgacgccgt tgccaatgtg tggacatgcc atgttatcgg caccacgatt gccgatagtg
    55681 acgccgtggc gaatggtgaa atcatccccc gctaccacgt ttttattgat cactacggcg
    55741 taaccgtgat ggatagtaaa gcggcggcca atggtcgcgg cggcctggat ttcataaccg
    55801 aaaaagcatt cggtgataat gcgatacagc accagcagcg gggccgccca cagattgttg
    55861 aggacgttct ttttgcgcca caccgaacaa aaatgagcaa cacgataggc aagaaccatg
    55921 cagcacgggc gtaaactcca gctgttggca cgcagatctt ccagcatctt tagcgccccc
    55981 gaataccatc agccagacgt ttgccgttac gcaccgacag cagcgtcagc aaagtgcgcc
    56041 aggtcatacg tttattgcgg atctggtaga gggtaaacag ctgatatttt ttgcttgcac
    56101 ggtcaaattt atctttgtgc tttcggtaaa agtggaagta cccggagaat tttttcggtg
    56161 acgaggtgat ctgcatttca ccgtgattga tatgcaggat ctgcgtcgct tcttccactt
    56221 tccacggttc gccgtactcc accaccatcc gcaggaagat gtcgtaatcc tgtgctgctt
    56281 tcagttcggt atcgaacaaa cactctttga aacgccatgc ccaagtaaag acctgattgc
    56341 caatgatatt gcgtttatag aacaggcggc gtgagtacgg cgatttggga tacagtggta
    56401 agcttgccgg ttgggaataa acttctccct ggcatacgta gtcgttagcg tacaaaaagg
    56461 cgtgagtgac cagttgctgt ttatgggcga ggaagacgct cagacggttg ggtgtccatt
    56521 cgtcatcgtc atcgatcccg gtaatgtatt ccccttgcgc cagcataatc gcctggttac
    56581 gtaccgcgca cgccccgctg ttaatgtcgt tgtgaatgta ggtgatacgc ggatcgttga
    56641 gggcagtgac gtactgttgc agttgttccc aggaggtgga gcaatcatcc acgatgatca
    56701 tctcccagtt gctgtagtcc tggcgcagga ccgattttat cgcccgaatc gccagttgtt
    56761 gacggttcca ggtcggcata tagattgaaa tcagcggatt ctctttcata ggttgttctc
    56821 ccggcttatg agctggtttg cttgccggat gcggcgtgag cgccttatcc ggcctacggg
    56881 gcggtgcgaa tgcaggcgtc gttatttcgc atccgactta tattcgtatt cgtaatagcc
    56941 ataatcctga tacgcgctgg cgcggcggaa gatggagttc agaatcaccc ctttcaccgg
    57001 aataccgttt tgctcaaagc ggctcagact ggtttccact tctttcaatg tgttgaccgc
    57061 atagcgcgcc accattaacg tggttccagc gtgacgcccg acaattgctg catcggtcac
    57121 tgccagaatc ggcggcgtat caatcaacac caggtcgtag tttttactcg cccagctcac
    57181 caattcggca aagcgttcgc tcatcaacag ttcagaagga tttggcggca cctgaccgcg
    57241 cgggatcagg tcaaatttgg caatagaggt cggtttagct gcggtagtaa tatcgccctg
    57301 gccaatcaga atttccgaca ggccattgac gttattggta cccaacagct cgtgggtgta
    57361 gcctttgcgc atatcgcagt cgatcaacaa cacccgttta ttggtctgac tgatcaccgc
    57421 cgccaggttg gcgcagacaa aggttttacc gattgacggg ctcacccctg tcatcatcaa
    57481 cacattgttc tgcgcctgca tcatcgcgaa gtgcagactg gtacgcagac tgcggatggc
    57541 ttcaatcgcc agatcggttg gattccccac cgccagtagc tggctctgtt tatagcgttt
    57601 aatccctttg atggttttga cgctatcgcg cgctttctgc cattccgaca gcgggatgct
    57661 ggcatagacg ctgataccat gttcttccag cacctgcggg ctttcgatgc cgcgattaaa
    57721 caacgagcgc agcagcacac ccacgataga gagcatcagg ccgaggataa ttgcgccgag
    57781 gataatcagt cctttcttcg gtttcagcac gccaggctgg gtgattgccg ggtcaacaat
    57841 gcgcacatcg ccgacggtgc tggcttcggt gattttcagc tcctgctctt tattcagcag
    57901 ttgcatatag acctgctgac cagactcgac atcgcgggtc aggcgcacaa tctcctgctg
    57961 ggttttcggc atcgccgtta cgcgaccgtt aagtttggct ttctcttctt ccagcgcctg
    58021 acgtttctcc agcagcgtgc ggtacgccgg gtgaactttg gtgtacagct tggagatttc
    58081 cgcctcttta aaggtcagtt cgttcaactg cgcgtcgatg ttcaccatcg aatcgagcac
    58141 cgctttcgct tccagcggca gatcaacaga atctttatcc tgacggaagg cattcagttt
    58201 gttttcggca acatcgaggc ggctacgtac ttccggtaac tgttgcgcga ggaaagcgag
    58261 gcttttcgac gcttccgccg atttgcgctc aatattttgt tcctgatagt tacgggcgat
    58321 gctgttaaga atgtcgcgga tctgttcgcg atcttcaccg gtataagtca ggctcagtac
    58381 gcctgcgtct ttgccgttct ccgttaccgt caggctgttt tgcagttggt tgatcatccc
    58441 caacgtggag tatttggtga cggtaaactc actgcccggg cgggcgtgaa tggcttcaac
    58501 catcagcgtg acgccttctt ttttcagcat ctggcccgct tgcccacggg cgctaaagcc
    58561 gccatcactg ctcagggtgt agtttttatt gtccagcaca ttaagcgtaa acacctggcc
    58621 cgccatctct ttcgggcggt taaaggtagt cactttcacc gtctcgttct ggcgtcccat
    58681 cagtcgctcc cagcccgccc cgaaaatcgg gaacgtgttt ttgctcactg caatatcgag
    58741 gtcgagatcg tccaccgttt taccaagcac cagacgcgaa cgaatcaact ggatctcggc
    58801 gtccgatgca ggcggtttgt tggctaatgc cgaaccaatg tcctgcacta acgaattgcc
    58861 gctgttttgc tcgatttgta ccagtgcgtc ggcactataa atcggcgttg cgaagaaggt
    58921 gtaaaccacg gcacagaggg caaatacagc ggtgatgcca atcacccacc agcgcgcttc
    58981 aatgacggtg ccgaccaggc gaccaatatc gatttcatca ctgcccgtta ccggagcggc
    59041 atgttgtttt actttttctg tcattcttat acctgctctg cgttcaatgc ctgcgcccac
    59101 tggcgggcag accgttcaag taatgtgtac accgctgcaa acgtttcccg gcttttgcga
    59161 tacggatcgg ggatttcaca ttcgttatcc cagtgaccaa acagcatcac tttgccgcgc
    59221 atctcaggtg ccatctcgca taagcgttcg atatggcgct tttccatggt caaaatcagg
    59281 tcatagttgc gacacaggcg gcggctgatt tgtcgtgcac agtggccttc cagagacagt
    59341 tgatgttctg cggcgacgct gatagcggta ggatcagcgc ccttaccgac cagcgcgccg
    59401 agtccagcgg actccacttt cagttccggg tgataacgtt gcagtaagcg ttccgccgtc
    59461 ggggaacggc aaatattgcc gacacagaca actaatatgt tgttaaacat gacgattacc
    59521 agttatgaat gtcgctggct gtatccgtca tgtaacggac accgctaata gttggcaaca
    59581 actgattgat cagacggttc cagcgggaaa ccggggcggt ggtgacatac accacgtcat
    59641 aaggttgcag gcggaattct gtcgccatca ccagcgacgt ggcatcggac atatccagct
    59701 ggtagatatt ggcaatcttg ccattacgcc cgccctcgcc tttcagcgga cgaatgacaa
    59761 agatgccgct ggcgttggag gtggtcatgt cgataccttc agcattaccc agggcttcag
    59821 tcagggtcat gccgctaaag tccattttca gggtgctctg tttcttcact tcacccatca
    59881 caaatacttt cagatcatca ttacgcggca cgtagagaat atcgccggga taaagcagtc
    59941 ggttctgatt gaggtcgccg ttttgcatca gcgcctgcaa agaaatgcgt tcttcacgac
    60001 cattgtgtgt tagcactacg ttgcgccagt cagcggtgtc ggtcaggcca cctgcggcgt
    60061 tgatggcgtc gagaatagtc agtggcacgt tggtgatcgc ctgttgaccg gatttattca
    60121 cctgaccgga gatataggcc ttttgtgagc ggaaggcggc gatattaacg tccacctgcg
    60181 ggtcagcgat gtacgtcgct aagcgcccgg taatatcact gcggatttca gcgagcgttt
    60241 tcccgactac gtgaaccttg ccgatatacg ggtaaaacat agtgccgtca ggctgtaccc
    60301 agttgccggt gtcgctggag ctgcggtact gaccggctgg cgtggtgagt tccgggtgat
    60361 cccagacggt gacattcaga acgtcgcccg gcccgacgcg atactggtaa ttcgcgatct
    60421 cgctttccag cgtcatattg gggcgcgcta cattcgggcg tgggcgtaat tggtcaatca
    60481 ggcgcggggt cagcggataa acattcacca ttttgtcgag atcgaaatca gcgtcctgct
    60541 gtttgatgac gtctttgccc atcgtcgaca tattgctgcc cggaagtact gtgcaaccgc
    60601 ttatcaaggt tactgacacc aataatggca tcaatttcat tttggatttc atcattgttt
    60661 atttatcact ttggcagagt aattatcctg tgcactatta atagcaatat cgccatgcac
    60721 atttaccttg cagttaattg aataaaaatt taactggcat cagtcctaaa aaaattgatt
    60781 tcatccgcag gctattgaca gaataattca gactggtctt tcaggcatcc agacacgcta
    60841 ccgcccctgg ctttttagcc accaacacac tgatttagct taatttttca aaccctctct
    60901 gcatacaatc gttgatgaga aagggttatt gcggaaatta acttccgaat ataaggtgac
    60961 attatggtaa ttgaatattg gctctccaat aatgcaggag gaagtgttac agctaacgga
    61021 atagcaggca aggtaacaat tcggcaattg gctattttta agaattatat taagtgtcat
    61081 tcaatatggt ttttaggagt ttctttaggt tgacaatatt taatattgcg cctccacatg
    61141 cgatatttct taaataatgt tttattatta ccacttttaa ttcagggata atgtgaggtt
    61201 attaccctca aataatatga gataaatagt gctgcaacat tgcatttttg ccccgattta
    61261 tccatgatcg aattgtgaca tttgtcatac aacgaatagg ttttgtactt actatggaat
    61321 ggattgccga tccgtctatc tgggccgggt taatcacgct gattgtgatc gaactggtcc
    61381 tcggcattga taacctggtc tttattgcca tcctcgccga aaaactaccg ccgaggcagc
    61441 gcgaccgcgc acgggttacc gggctgctgc tggcgatgtt aatgcgcctg ttactgctgg
    61501 cgtcaatctc ctggctggtc accctgactc aaccgctgtt cagcttccgc tcgtttacct
    61561 ttagcgcccg cgacttaatc atgctgtttg gtggtttctt cctgttgttc aaagccacga
    61621 tggagctgaa cgaacggctg gaagggaaag acagcaataa tcccacacaa cgcaaaggtg
    61681 cgaagttctg gggcgtggtg acgcaaattg tggtactgga cgccattttc tcactcgact
    61741 cagtgattac cgccgtcggg atggtcgacc atttactggt catgatggcc gccgtggtta
    61801 tcgctatcag cctgatgttg atggccagca agccgttaac gcaatttgtt aacagtcacc
    61861 cgacgatcgt tattctctgc ttaagcttcc tgttgatgat tggctttagc ctggtggcag
    61921 aaggtttcgg cttcgtcatt ccgaaaggct acctgtacgc tgccattggt ttctcggtga
    61981 tgatcgaggc gctcaatcag ttggctatct ttaaccggcg acgttttctt tccgctaacc
    62041 agacgctgcg ccagcggact accgaagcgg taatgcgcct gcttagcggg caaaaagaag
    62101 atgcggaact ggacgccgaa accgcgtcca tgctggtgga tcatggtaac cagcagatct
    62161 ttaatccgca ggaacggcgg atgattgagc gggtacttaa tcttaaccag cgtaccgtca
    62221 gcagcattat gacgtcgcgc cacgatattg agcatatcga tctcaacgcg ccggaagaag
    62281 aaatccgcca gttgctggag cgaaatcagc atacgcggct ggtggttacc gacggtgatg
    62341 acgcagaaga tttgctcggt gttgttcacg ttatcgacct gctacaacag tctctgcgcg
    62401 gcgaaccgct caacctgcgg gtgttgattc gccagccgct ggtgttcccg gaaaccttgc
    62461 cgttgttacc tgccctggag cagttccgta atgcccgcac gcactttgct tttgtggtgg
    62521 atgagtttgg ctcggtggaa gggattgtga cattaagtga cgtcactgaa accattgtcg
    62581 gtaacttacc gaacgaagtg gaagagatcg acgcccgcca tgatattcag aagaatgcgg
    62641 acggttcctg gacggcgaat ggtcatatgc cgctggaaga tctggtgcaa tatgtgccgc
    62701 tgccgctgga tgaaaaacgt gaatatcaca ccattgccgg gctgttgatg gaatatttac
    62761 agcgtattcc aaaacctggc gaagaagttc aggtggggga ttatttgctt aaaacgttgc
    62821 aggtggaaag ccatcgcgtg cagaaggtgc agattattcc gctgcataag gatggcgagt
    62881 tggagtacga ggtgtgatgg tatcggtcat tttacccaat agccgaaagg aataagatga
    62941 gggaaccgcg ccagcggcga tttaccgccg ggagcccggg ttgccagggt ggtggcgatt
    63001 gagccaccct ggcacgttca ccggcttgag cgttccatag tagcgaggaa catcaagtga
    63061 acggaacagc cttcgatctt acatattcag aatataatga tgccgccacg aacgcctgat
    63121 gcgacgcttt acgcgtctta tcaggcctac aggactggtc acctggtaac ctacatcttc
    63181 tccagcaact tcttcacatc cttgccattg cgggaatctt tattccgctc ggcccagtca
    63241 ttcaggcgac gtttcgcttc gtcctgtaga tgtttgcgca gtaactgatc cacttgcagg
    63301 ctgtaattga gttgctgcca gttgccataa acccgcagcg gtactggcgt ttctttcagg
    63361 aaatcaatca gtttactttc cccgttccag ccacccacga cccgaatatc aaactgggta
    63421 tcgcaggttt gatctgccag attcaacatg ccttcccctg tcagcgccag cacaggcgat
    63481 tgaccttgca tgtcgtttaa cgtcacgacg ccatccttca acgtcaaatc ggtggtaaag
    63541 cggtcaagcc gcgtcacgtt atcaaagttt tcagcggcct tcacatcacc gccattacgc
    63601 tctaccgctt gctgaatcat ctgctggaag ttcatccctt ccatgcgcgt gtcggtcatt
    63661 tcgacatgtg cctgtccttg ccagttgtgg cggaatgcgt cggcatctat gtcagcaccg
    63721 gagaaatcac cagccagtga catttttccg gtcaacgaaa tcggatagtt aaacgccttc
    63781 agaatggtac caatctcaac gttttccagc cgtggctgga agtttatccg cggatttatt
    63841 gatgtcgcgt ccagcgtgcc cggcagtgaa acctgtccac cgttaagttt gccctgcagt
    63901 tgagtaattt ccagcaaacc cgacttgttg gtcatttgcg tggcaacatc tgtaaaattc
    63961 attccgcgcc agcgcacgtt actggcctgc aacaaaatat cagccgtaaa gccttgcagt
    64021 ccctgatagg ccggttcatc aatacgcgaa gaaatgaccg ggcgcggcaa cgttgattgg
    64081 ctctgcccct gctgcgcggc accgttttca ccattcgcgg tttcattaag cgggatgagg
    64141 ttgtcaagat tcagttgcgg gaattgcagt ctcagctgcc attccggttt ctctgtcagc
    64201 gtgacctgtg cttgcccgct cagcgtactg tcattggcgg tcaagctgat ttggttaaat
    64261 gaaaggcgtt tatgtgactc ctgccactgg gcctggaaac tcccctgccc ctgaatacct
    64321 tgttttggta aatcggcacc ctgcaactgc cagttaattt gttcaatagc cgccgttaaa
    64381 tcatgcggat aatcagaagc atccaccgta ccgttaaggg atattgtgag atcgcgctga
    64441 tcgcgattaa cccgcccgga gaactcaaat gagccacgat gttggggatc ttgttccatt
    64501 tgcaggcgga tattgcggat tgtcacctgc tcgtcatctt catgctggaa caccagcacg
    64561 ctgtccgcca ccttaagact ggatatatca aacgaccatc cgcgatcgtc tgacagatcc
    64621 ggcaaggtat tgtcgcgcgg tgcaaccgga gcgtcttcac tgcgcaccgc ttccgtctgc
    64681 ggcgtcagtt ggatcactgc cccttttagc atcacctgct taacgctcag ttgatgactc
    64741 agtagtggta aaagcgccac gtccagacgc atgttgtcgg cgcgaaccag tggctggctt
    64801 gcgccctggg cggtgagaga cattcgcccg gagaggatac taagctgcgg ccagacgtgc
    64861 caacgcagtg gcccgtcgag ctgcaattga taaccgctac gtgcagcaac ttgcttgacc
    64921 atatagtcgc ggaaatcatt cggattcacc agcaacacta acacagataa cccggccacc
    64981 agcacgacca ggagtatcat cagcgtcgtc agaaatcgtc tcatggtatc ctcaatgggc
    65041 ctgaattagt ctttatcgat tcggctggct accgcgccct gctggttgcg atatttcgca
    65101 tcttcacggc ggttgtaagg tcgcgccgcc gggccggaaa gcggctcaaa gctcagcgca
    65161 ccaattaaca tgcccggacg cagcgccagc ggcagcttac cggagttgta gaactccagc
    65221 acaatgcaac cagaccagcc cggatcgata cggtgcgcgg tgacgtgcac catcagcccc
    65281 agacgcgcca aagaggaacg cccgtccagc cagcccacca gatcggctgg cagcgtcacc
    65341 gactccagcg tcaccgccag cgccagctct cctgggtgaa gatagaacgc ctcgccctcg
    65401 tcgagaacga tctcatcgct catcacgcgg tcaagcgcgg cgctcacttc atctttggga
    65461 ccgctcagat cgataaacgc tgccgtgtga ccacggaagg tacgaaattt attgcccagg
    65521 cgtacatcca ccgtcgcgcc gttaatacgc tccactggcg gacgtgggtt gatcgacaaa
    65581 cggccttcat caagccaggc ttcaatatct cggtcacaca gacgcatggc attttctcct
    65641 ttcgcgcatc actcccttaa cgccaattac gtcaagggca taccaggtta tcactgaacg
    65701 gtacacaatt tatcaagctt attcaaagaa ctgacttatt ttcgctttca atatatcgat
    65761 cgcgatgcgg tttttcccgc cgcgcggcac gataatgtcc gcatattgtt tagaaggctc
    65821 aatgaattgc aggaacatcg ggcgcacggt tttttgatat tgcgccatca cggaatccat
    65881 tgaacgccca cgctcgttaa cgtcacgctt gatgcggcgc atcaggcaga tatccagcgg
    65941 ggtatcaacg aaaatggaga agttaagttc gtcacgcaaa cgcgcatccg tcagcaacaa
    66001 aatgccttcg agaatgatga ccttcttcgg ctcaaccgtc accgtttctt tcatacgcgt
    66061 atgttcaaca tagctgtaaa ccggcaggtc aattgccgag ccgcgtttca acgcttgtaa
    66121 atgctcaagc agcaggctgt gatccatcgc gctgggatgg tcgtagttgg tcttaacgcg
    66181 ttcttccatc gacagatggc tttgatcttt gtaatagcag tcttcgggaa ttacgccgat
    66241 gtgttcatca ccgacttgct cacgtaattc acgataaagg gtactggcaa taagactctt
    66301 gccggaagcc gatgcgccag cgataccgat aatgacgcac tgatgagact gatcagtcat
    66361 atatttagcg acctgattaa cctggatgtt aggaaggggg cgacgaagcg ccaaacgcgg
    66421 caattatagg gatttcatcc gtctgatacc agtcgaatag cgttgccgcg cgctcagagt
    66481 taattgttga caaagaattc ccggtggcaa attacgttga tcagttttat gtaaggtaaa
    66541 aaatgttata cgcagttgcg caaattatcc gcctttacgt cactttatga gcaattcgca
    66601 tataaaatgt aaaacttttg tactagcata aacacagaaa cgaatactgg cgacctggtc
    66661 ttgcggataa agcggtaatg agcaaacaat cacagcatgt attaattgcc ctgccccacc
    66721 cgctgcttca cctggtcagt ttaggtttag tctcgtttat ctttaccctt ttctcgcttg
    66781 agctttcgca gtttggcacc caactcgccc cactgtggtt cccgacgtcc atcatgatgg
    66841 tggcgtttta tcgccatgcc gggcgcatgt ggccgggaat tgcgctgagc tgttcgctgg
    66901 gaaatatcgc cgcatccatc ctgctttttt ccaccagctc gctgaacatg acctggacga
    66961 ccatcaatat tgttgaagcc gtggtcgggg cagtgctgct acgtaaattg ctgccgtggt
    67021 ataacccctt gcaaaatctg gctgactggc tgcgtctggc actcggcagc gccattgttc
    67081 cgcctctgtt ggggggtgtt ctggttgtcc tgctgacgcc cggagacgat cctctcaggg
    67141 catttttgat atgggtactg tcagaatcca tcggcgctct ggcactggtg ccgctgggat
    67201 tgttatttaa accacactat ctgctgcgcc atcgcaaccc acggttgctt tttgagtcgc
    67261 tgctcacgtt agccatcaca ctgacgttaa gctggctttc gatgctgtat ctgccgtggc
    67321 cttttacttt cattattgtg ctgttgatgt ggagcgccgt gcgcctgcca cgaatggaag
    67381 cctttttgat cttccttacc acggtgatga tggtgtcgct gatgatggcc gcggatccct
    67441 ccctgcttgc tacgccgcgt acgtacctga tgagccatat gccgtggcta ccgtttttgc
    67501 tgatcctgct gcccgccaac atcatgacca tggtgatgta tgcctttcgt gcggaacgca
    67561 aacacatttc cgaaagcgaa acccgctttc ggaacgcgat ggaatattcc gctatcggta
    67621 tggcgttagt gggcaccgag ggacaatggc tgcaaaccaa caaagcgctc tgccagtttc
    67681 tcgggtacag tcaggaagag ctgcgcggac tcacctttca gcaactgacc tggccggagg
    67741 atctcaataa agatctccaa caggttgaaa agctgataag cggtgaaata aacacctatt
    67801 caatggaaaa acgctactac aaccgcaatg gcgatgttgt ctgggcgttg cttgccgtct
    67861 cactggtgcg ccacacggat ggcacgccgc tctattttat cgctcagatt gaagacatta
    67921 acgagctaaa acgcaccgaa caggtcaatc agcaactgat ggagcgcatc acgctggcta
    67981 acgaagcggg cgggattggc atctgggaga gggagttgaa gccgaatgtt tttagctggg
    68041 ataagcggat gttcgagctg tatgaaattc ctccacatat caaaccgaac tggcaggtgt
    68101 ggtacgagtg cgtgctgccg gaagatcgcc agcatgccga aaaagtgatt cgtgattcgt
    68161 tgcaatcacg ctcgcccttt aaactggaat ttcgcattac cgtaaaagac ggtattcgcc
    68221 atatccgcgc cctcgccaac cgggtactga ataaagaagg cgaagtcgaa cgtctcctcg
    68281 gcatcaatat ggatatgacc gaagtgaaac agcttaacga ggcattgttt caggaaaaag
    68341 agcgcctgca cattacgctt gattccatcg gtgaagccgt ggtctgtatt gatatggcga
    68401 tgaaaattac ctttatgaat ccagtggcgg agaagatgag cggctggacg caggaagaag
    68461 cgttaggtgt tccgctcctg acggtgttgc atattacttt tggcgacaac ggaccattaa
    68521 tggagaacat ttacagtgcc gacacctcac gttccgcaat cgaacaagat gtggtgttgc
    68581 actgtcggag cggcggcagt tacgacgtgc attacagtat tacgccgtta agtactctgg
    68641 acggcagcaa tattggttcg gttctggtga ttcaggacgt caccgaatca cgcaaaatgc
    68701 tgcgccagct gagctacagc gcctcccatg atgcactgac gcatctcgcc aaccgcgcca
    68761 gttttgagaa acaactgcgt atcctgctgc aaacggtaaa cagtacgcat cagcgacatg
    68821 ccctggtgtt tatcgatctt gatcgcttta aagcggtgaa tgacagcgcc gggcatgcgg
    68881 cgggcgacgc tttgctgcgc gaactggcgt cgttgatgct gagtatgctg cgctccagtg
    68941 acgtgctggc gcggctcggc ggtgatgaat ttggtctgct gctgccagac tgcaatgttg
    69001 aaagcgctcg ttttatcgct acacgtatta tcagtgctgt gaatgactat cactttatat
    69061 gggaaggccg ggtacatcgg gtaggtgcca gcgccgggat taccttgatc gatgacaaca
    69121 atcatcaggc ggcagaagtg atgtcgcagg ctgatatcgc ctgttatgcc tccaaaaatg
    69181 gtggacgggg ccgggtgacg gtttacgaac cacagcaagc tgccgcacat agcgaacggg
    69241 cggcgatgtc gcttgatgaa cagtggcgga tgattaaaga gaatcagttg atgatgatcg
    69301 cccacggtgt cgcttcgccg cggatcccgg aagcgcgtaa tttgtggctg atttcgctta
    69361 agctctggag ttgcgaaggc gaaattattg atgaacaaac atttcgtcgt agcttcagcg
    69421 atccggcact tagccatgct cttgaccgac gggtattcca cgaatttttc cagcaggccg
    69481 ccaaagcggt tgccagtaaa ggcataagca tcgccctccc cctttccgtt gccggtttga
    69541 gtagcgccac gctggtgaat gatctgcttg agcagctgga aaatagccct ctaccaccac
    69601 ggttattaca tctgattatt ccggctgaag cgattttaga tcacgcagaa agcgtgcaaa
    69661 aactgcagct ggcgggatgt cggatagtgc tcagccaggt gggccgcgat ctgcaaatct
    69721 tcaactcgct gaaagcgaat atggcagatt acctgctact tgatggtgag ttatgcgcca
    69781 acgtgcaggg taatttgatg gatgagatgc tgattacgat tattcagggg cacgctcagc
    69841 gactcgggat gaaaaccatc gccgggccag tcgttttacc cttagtgatg gatacgcttt
    69901 ctggcatcgg cgtcgatctg atttatggtg atgtgattgc cgatgcccaa ccgctggatt
    69961 tgctggtgaa tagcagttat ttcgcgatta actgagagag cgtttgccca ttgcctgatg
    70021 cgacgctaac gcgtcttatc atgcctacaa atcgctcatt ccccagaccg gataaggcgc
    70081 tcgcaccgca tccggcgacc aacgtcatgc ttcgtctggt tgccagcctt ccgtatacca
    70141 gatatgcaac agcgcataag aacgccaggg cttccagcgc tcggcataac ggcggatttg
    70201 tgccggtgtc attcccggaa atcgccgttt aatcagataa tcatccggca gaaaaacatc
    70261 tttcgcctgc cagccacgca aagcaaaata attcgccgtc cagcgcccga tacccggaaa
    70321 agtttgcagc gttttcatcg cctgctccac atcgcccgga attgtcattg gtaagctgcc
    70381 ctccagcgcc gcatttgcca gatgaatcag tgcctctgcc cgtttcaacg gcatgcctaa
    70441 cgcttttaat gcctgcgggt cggctgctgc cagccgctga ggcgtgggga agcagatata
    70501 ctccggaaaa tcatccagcc gttcgccata aagctgtacc actttggcag tcaattttgc
    70561 cgccatcgcc acgctcacca gttggcctaa aatcgcccgc acaccctgct caaaagcatc
    70621 aatacagccg ggtaaacgca atccgggccg ccccgcgcct aactttccca acgcaccgtt
    70681 aacaatctgc gggttacatt gcagatcaag caggcggctc attttcgcca gacactctgc
    70741 ggcaacaggt tctaaacctg cacttaaatt tatgtgcaga gtatggcggg ctatatccgg
    70801 aatagcagtc actacgccgc gatattcgcc caccgccaga ctacgggcat aataattgtc
    70861 cgcgaccgtt tccacaccgc tcaccgcacg ggcggcgaga aatcccaaca tccacgacca
    70921 gtcatacggc ggctgccagt tcagggtata catcgcatct ccttgttaat ccgctttcag
    70981 cataaacgtt attcagacgt tacgctttgc tttcatattc cggttgtcgc gacagcaaca
    71041 tttcgctaaa gtcacgcccc ttcttcaccg gcatggggat taattcgtgt ttattggttt
    71101 tgattacggt acagcaaact gttcagtggc ggtcatgcgt gacggtaaac cgcaattact
    71161 gaaaatggaa aacgacagca cgctgctgcc ttcaatgctt tgcgcgccaa cgcgtgaatc
    71221 ggtaagcgaa tggctgtacc gccatcatga tgttccggca gacgacgatg aaacgcaggc
    71281 gctgttacgt cgggcgattc gttataaccg cgaagaagat atcgatgtta cggcgaaaag
    71341 cgtgcagttc ggtctttcct cactggcaca gtacattgat gatccagaag aagtgtggtt
    71401 tgtgaaatca ccaaaatcgt tcctcggtgc cagcggctta aaaccgcagc aggtagcgct
    71461 gtttgaggat ctggtctgcg caatgatgtt gcacattcgc cagcaggcgc aggcgcagct
    71521 gccagaagcg attactcagg cggtgattgg tcgcccgatc aacttccagg ggctgggcgg
    71581 cgatgaagca aacgcccagg cgcaagagat tctggaacgc gcggcgaagc gtgccggatt
    71641 cagggacgtg gtattccagt acgagccgat cgcggctggg ctggattacg aagccacctt
    71701 gcaggaagaa aaacgggtgc tggtggtgga tatcggcggt ggtacgactg actgttcatt
    71761 gctgctgatg gggccgcagt ggcgttcgcg tctcgatcgt gaagccagtc tgctgggtca
    71821 cagtggttgt cgtattggcg gtaacgatct ggatatcgcc ctggcgttta aaaacctgat
    71881 gccactgctg ggtatgggtg gcgaaaccga aaaaggcatc gccctgccga tcctgccgtg
    71941 gtggaatgcg gttgccatca acgacgtacc tgcgcagagt gatttctaca gtagtgccaa
    72001 cggtcgtctg cttaacgatc tggtacgcga tgcccgcgaa ccggaaaaag tggccctgtt
    72061 acagaaagtc tggcgtcagc gtttaagcta tcgcctggtg cgtagcgcag aagagtgcaa
    72121 aattgctctt tcaagcgtag cggaaacccg cgcctcactg ccgtttatca gcaatgaact
    72181 ggctacgctg attagccagc gagggctgga aagcgccctc agccagccac tggcgcggat
    72241 tctggaacag gtgcaactgg ccctggataa cgcccaggaa aaaccggacg ttatctacct
    72301 gaccggcggt agcgcccgtt ctccgcagat taaaaaagcg ctggcagaac agttgccggg
    72361 cattccgatt gcaggcggcg atgactttgg ctccgtcacc gccgggctgg cacgctgggc
    72421 ggaagtggtg tttcgttaat tgaaaaatgg ctcagcagcg gcttaaatct gcctgcgcct
    72481 gagccagttt ttctgctgcc tgacgtaacg gttccatcaa cggcatggtc cgtgctggag
    72541 catgaaggcg gaatcgctgc aactctgtcg cgccgactgt caacgccgat tccagccgat
    72601 gtcgtttggc ggtcattttc gccattaccg cttgtctgtc tgctggcgtt atcgcttcat
    72661 tcggcctgaa cacaaaacgg cgttcacagt tcgctttcca gtcgataacc gcctgggtca
    72721 gatggtcgcc aaaccctttc acttgcttaa cgccacgacg ggtaacatct gcggctgttt
    72781 caataccaaa agaccgtaac gccgctttac gcgcagggcc aacgccggga atagaggcaa
    72841 catcaataaa aaatccctcc agaaacttct gcttctgccg ttcccttgca gtgtcatgaa
    72901 gtgctgccag agcgcgtttc tcttcttccg gtaacccgag aatttcgtcc ttcatttttt
    72961 cgagcatcgt ccgtttggcg ataaaacctt ccaggccgcc tacctgctgg atctggcgga
    73021 ctaaatggtc atagtccatt ttcgcacgat taaatgcctc acggcgttgc tgaacttctg
    73081 ctttgtacgc tttgcttgtc aaactgccaa taatccagat agccgccagc acaggaacca
    73141 aaataatata acgcggttct gcctgaaggc cgcaaagcag cagcgataat gctgacagtg
    73201 cgatctcaag cagaatgatg tattcgcgcc gtaacaggcc taaaggaagc ggcctgccag
    73261 tcggttggaa atgatcgggt aatggcagtt gcaatgctgg cggtgctact gacgccatca
    73321 ccatcgccca gacttttgcc agcacaaaat ccccgccggt ggtaatgacc tcttcgccga
    73381 gatcaataaa atagataaca ccttgattat ccagcgcaca ccatgggcag tcagccaaat
    73441 gaccgggata aacatgcatt gccgaaacgg tacatttctt taactgttgg cgtagagaat
    73501 ccagtgctga tacccacgct ttagccgtcg gacgcccggt tgccacgcca ctttccgtaa
    73561 acgcctgctg aaacatggct tcaacatcac tcggtaacat cgatagcgga atcgatcgtg
    73621 gcggcggttt taaaccacgt cgctgattat ctgacgcgta ggcataacgg aaatgggtaa
    73681 tatccgtctc cagcgcatta cccgcatcag agataagcgg cacaccagaa taaggatgcc
    73741 ggccaccaaa caagacgtga aaaatcagca acgcaaggcc aaaattatcg tgattttctg
    73801 tgcgttcaaa gccgacaaat gacgacaagg tttgtagctc tggcggcgta aaatgcgaca
    73861 cgccgacttc gcataaatgc agtgtgccat tggcgttaat ctgaaaggag tcgctatcaa
    73921 tcaacaccac tttgctgtcg cgacctacca taaaactgtt ctggtttacg tcacccacga
    73981 cgtgcccgtg ctcgtgaacc gtagcaaaag atgaagcaat attgcgcgca acatagagta
    74041 gaaaatccca cgcacaatga gggtaacgtt gacgacgatg tgccgggcta tagatcatat
    74101 gaatcggttc tttaccagaa acttttggca tcataaaacc gataactttt ccgcctcgtc
    74161 caccgtgaag cgttgcctgc ggccaggcga cataattcaa caattgcgcg tcagctgtcg
    74221 cagccataaa ggcaagtttg tcctgtttta aggcgggtgg cggcgtgtga taaatcttgg
    74281 cgacgctatc gacaaactca ttgatatcat aaaccgcgcc ttcaccgcct ttgcccagtt
    74341 cacggcctgg cgtaacgcac tcaccagtag cagtatataa agtgggtttc atggtaacca
    74401 cagcgccagt gccaacgttt tatcatcatc cgtacgctcg tttaccgccg ggctggacaa
    74461 aaactgcttt aacaattcag gtaataaatc gagttgttcc tgcgttgctg acgccagtcc
    74521 attgaaaaac ggggtaaaga aaggcacatg aggagaatta tccagcatat ttaacgccag
    74581 ccgctgaata ccatccgtaa atgctgcaac tttatgcgca cgcccagtgc tggtgaaagt
    74641 ttccagtctg gaaacggcat cttcgtcggt aatgaagtgc gtcatgttgg catattcacc
    74701 caccatcggc gtgagaggca attgcagacc gtgacccaaa tctaccacca cgccgccatc
    74761 gccaatctgc ataatcaagg tgccgtcagg cgatgaaatc aatcccagaa acgtgcaggc
    74821 aaaatcgcga actgctaatg ctttggcttc tgcctcggca aaaagtcgtt gacgaatagt
    74881 cagtaccata tttgtggcga ggacatcatt aagccccaat tcaccgtcct gtactttttg
    74941 caccataaac gccatcgctt cattgacggc gagcgtcgcg ccttctcctc cctgcgaaac
    75001 gctacctgcg ccatcagcaa ggaacatcac cagtaatggt tgctggtcgt ttaaccagac
    75061 aacctgcatc tggcaggcat cctgacaagg caaatcagca ctaatgtgcg atgttccaac
    75121 agcggatgca taaaccagac gccagctcac actgacgtcc agccttttgg cgcttccagc
    75181 acaacttccg ttcccggcgt ggagcgggaa accgaacgca gcgaactgga taaccagcta
    75241 aacagttcac ggaattgtaa tccctgcagt ggcaaaggct gacgaacgct gatttgtgcc
    75301 agagtcttca tatccgcccc ctgaacaccg atggaaaaga aggcaaactt cttatcttct
    75361 tccccctgaa acactttgtt ggcggcagcc tgccactcat cggttggagc gccatcggtg
    75421 atcaggaaaa tccacgggcg ataatacgaa ataccatttg cgcggtactc acgtttcctc
    75481 tcttcaacca tatccagggc tttggtaatg gcggccccca taggcgtatc cccctgggca
    75541 aacaggatgg gcgggaaaaa gttagcggcg ctggtaaagg gttgttccac atgcaccggg
    75601 ccgaacgtca caatgccgag ttccactctt tttaaagcca gcggatcagc aagcagttca
    75661 tcacgaaagg taaccaatcc ggcgttaagt tcattaatag gtctgccgct catcgagcca
    75721 gaaacatcca gcagcaaaat gcaggggcaa cgtggttccg ggttactggc aaaatcgctg
    75781 gtggcaaacg tgatttgttc gctcataaga aatcctttct tagagaatta tcgaggtccg
    75841 gtatcatacc gattgcaata atgaaataag agcaaacgaa gaaactttca gaattaggcc
    75901 cgtcggcgca gaaaattttg ctattcgcct cgcaagaacg gagagccgtc gattgacact
    75961 aagggcggag tgacataatt tcaggagtga gggttaggga gaggtttccc cctccccctg
    76021 gtgttcttag taagcctgga agctaatcac taagagtatc accagtatga tgacgtgctt
    76081 catcataacc ctttccttat taaaagccct cttctccggg agagggtttc ccgtttcagc
    76141 gtcccgctga aatcgtcggc ttacctcctt tcgccctgaa tgcagtctat cgctaacgcg
    76201 tgaacaaaat agtttcatct gtgattattg cgtaacgact cgcaaagaat atttgctggc
    76261 aggatcgcag actacaaagc ctgcggattg acaatcttat cgtgaaggca taatttcagg
    76321 agtgagggta gagcggggtt tcccccgccc tggtagtctt agtaagcggg gaagcttatg
    76381 actaagagca ccacgatgat gagtagcttc atcatgaccc tttccttatt aaggcccctt
    76441 cctcgggagg ggctttcccg tttcagcgtc ccgctgaaat cgtcggctta cctcctttcg
    76501 ccctgtatgc agtctatcgc tgacttgcgg ataaaatagt ttccagtgtg attattgcgt
    76561 aacgtctcgc aaagaatatt tgctggcagg atcgcagact gcaaagcctg cagattgaca
    76621 atcttatcgt gaaggcatac tttcaggagt gagggtagag cggggtttcc cccgccctgg
    76681 tagccttagt aagctggcca gctaatgact aagagcacca cgatgatgag tagcttcatc
    76741 atgacccttt ccttatttat ggccccttcc tcgggagggg ctttcccgtt tcagcgtccc
    76801 gctgaaatcg gcggcttacc tcctttcgcc atgcaagcag tctatcgcta acgcgtagat
    76861 aaaatagttt cctgtgttat tactggatgc gtgctcgcaa atgtgcccgt cattcagacg
    76921 attcccgaca gtgtttcata attcctccat ttttctccct tattggctgg ctacactagt
    76981 atcattccgc gaaacgtttc aggaagagaa actcttaacg atgaaaggca gttataaatc
    77041 ccgttgggta atcgtggtgg ttatcgccgc catcgccgca ttctggttct ggcaaggccg
    77101 caatgactcc cagagtgcag ccccaggggc gacgaaacaa gcgcagcaat cgccagcggg
    77161 tggtcgccgt ggtatgcgtt ccggcccatt agccccggtt caggcggcga ccgccgtaga
    77221 acaggcagtt ccgcgttacc tcaccgggct tggcaccatt atcgccgcta atactgttac
    77281 ggtgcgcagc cgcgtggacg gtcaactgat ggcgttacat ttccaggaag gccagcaggt
    77341 caaagcaggc gatttactgg cagaaattga ccccagccag ttcaaagttg cattagcaca
    77401 aacccagggc caactggcaa aagataaagc cacgcttgct aacgcccgcc gtgatctggc
    77461 gcgttatcag caactggcaa aaaccaatct cgtctcccgt caggagctgg atgcccaaca
    77521 ggcgctggtc agtgaaaccg aaggcaccat taaggctgat gaagcaagcg tcgccagcgc
    77581 gcagttgcaa ctcgactgga gccgcatcac cgcaccagtc gatggtcgcg ttggtctcaa
    77641 gcaggttgat gttggtaacc aaatctccag tggtgatacc accggaattg tggtgatcac
    77701 ccagacgcat cctatcgatt tagtctttac cctgccggaa agcgatatcg ctactgtagt
    77761 gcaggcgcaa aaagccggaa aaacgctggt ggtagaagcc tgggatcgca ccaattcgaa
    77821 gaaattaagt gaaggcacgc tgttaagcct cgataaccaa atcgatgcca caaccggtac
    77881 gattaaagtg aaagcacgct ttaataatca ggatgatgcg ctgtttccca atcagtttgt
    77941 taacgcgcgc atgttagtcg acaccgaaca aaacgccgta gtgatcccaa cagccgccct
    78001 gcaaatgggc aatgaaggcc attttgtctg ggtgctgaat agcgaaaaca aggtcagcaa
    78061 acatctggtg acgccgggca ttcaggacag tcagaaagtg gtgatccgtg caggtatttc
    78121 tgcgggcgat cgcgtggtga cagacggcat tgatcgcctg accgaagggg cgaaagtgga
    78181 agtggtggaa gcccagagcg ccaccactcc ggaagagaaa gccaccagcc gcgaatacgc
    78241 gaaaaaagga gcacgctcct gatgcaggtg ttacccccga gcagcacagg cggcccgtcg
    78301 cgcctgttta ttatgcgtcc tgtcgccacc acgctgctga tggtggcgat cttactcgcc
    78361 gggattatcg gttatcgcgc cctgcccgtt tcggcgctgc cggaagtgga ctatccgacc
    78421 attcaggtgg tcacgctcta cccaggtgcc agcccggatg tcatgacctc tgccgttacc
    78481 gcgccgctcg aacgccagtt cgggcagatg tctggcctga aacagatgtc gtcgcaaagt
    78541 tccggcggtg cgtcagttat cactttgcag ttccagctaa cattaccgct cgatgtcgcc
    78601 gagcaggaag tgcaggccgc gattaacgct gcgaccaact tgttgccgag cgatctgcct
    78661 aacccgccgg tttacagcaa agtgaacccg gcagatccgc cgatcatgac gctcgccgtc
    78721 acctcaaccg ccatgccgat gacgcaagtg gaagatatgg tggaaacccg cgtcgcgcag
    78781 aaaatctcgc agatttccgg cgtcggcctg gtgacgcttt ccggcggtca gcgtccggct
    78841 gttcgcgtca aacttaacgc tcaggcgatt gccgccctcg gcctgaccag cgaaaccgtg
    78901 cgcaccgcca ttaccggcgc taacgttaac tcggcaaaag gtagcctcga cggcccttcc
    78961 cgtgcggtca cgctttccgc gaacgaccag atgcaatccg ccgaagagta tcgccagcta
    79021 atcatcgcct accagaacgg cgcgccaatt cgtctgggcg atgtcgcaac tgtagagcaa
    79081 ggtgcagaaa acagctggct cggcgcgtgg gcgaacaaag aacaggccat tgtgatgaat
    79141 gttcagcgcc agcccggtgc taacattatc tccaccgccg acagcattcg gcagatgctg
    79201 ccacagctca ctgagagtct gccgaaatcg gtgaaggtga cagtgctttc cgatcgcacc
    79261 accaatatcc gcgcatccgt cgatgatact cagtttgaat tgatgatggc tatcgcgctg
    79321 gtagtcatga ttatctacct gtttttgcgc aatattccgg cgaccatcat tcccggtgtt
    79381 gctgtaccgc tgtcgttaat cggcactttc gcggttatgg tgtttctcga tttttcaatc
    79441 aataacctga cactgatggc gttaactatc gccaccggat tcgtggtcga tgacgccatc
    79501 gtggtgatcg aaaacatttc ccgctatatc gaaaaaggcg aaaaaccgtt ggcggcggcg
    79561 ctcaagggcg caggtgaaat cggctttacc attatctcgc tgaccttctc actgattgcg
    79621 gtgttgatcc cactgctgtt tatgggcgat atcgtcgggc gactgttccg cgaatttgct
    79681 attaccctgg cggtagcgat tttgatctca gcggtggtgt cgctgaccct gacaccgatg
    79741 atgtgcgcgc ggatgctcag ccaggagtcg ttgcgtaaac agaaccgctt ctcccgtgcc
    79801 tcggaaaaaa tgttcgacag gataatcgcc gcctatggtc gtggactggc gaaagtgctg
    79861 aatcatccgt ggctgacctt aagcgtggca ctcagcacgc tgctgcttag cgtgctgctg
    79921 tgggtgttca ttccgaaagg tttcttcccg gtacaggaca atggcattat tcagggcact
    79981 ttgcaggcac cgcaatccag ctcctttacc aatatggccc agcgacaacg ccaggtcgcg
    80041 gacgtgattt tgcaggatcc ggcagtgcaa agcctgacct catttgttgg cgttgatggc
    80101 actaacccgt cgctgaatag tgcacgttta caaatcaacc tcaaaccgtt ggatgaacgt
    80161 gatgatcggg tgcaaaaagt catcgcccgt ctgcaaacgg cggtagataa agtgccgggc
    80221 gtcgatctct tcctgcaacc aacgcaggat ctgactattg atactcaggt cagccgcacc
    80281 cagtaccagt ttaccttgca ggccacctca ctggatgcgc tcagtacctg ggtgccacag
    80341 ttgatggaaa aactccagca actgccacag ctttctgatg tctccagcga ctggcaggac
    80401 caagggctgg tggcgtatgt caatgttgat cgcgacagcg ccagccgtct ggggatcagc
    80461 atggcggatg tcgataacgc cctgtacaac gcgtttggtc agcgactgat ttccactatt
    80521 tatactcagg ctaaccagta tcgcgtagtg ctggagcaca acaccgaaat taccccgggc
    80581 ctcgcggcgc tggataccat tcgcctgacc agtagcgacg gcggcgtggt gccgttaagc
    80641 tcaattgcca aagttgaaca gcgttttgcg ccgctttcca tcaaccatct ggatcagttc
    80701 ccggtaacga ccatctcctt taacgtgccg gataactatt cgctgggcga tgcggtgcag
    80761 gagattatgg acaccgaaaa gacgctgaat ctgccggtgg atatcaccac gcaattccag
    80821 ggcagcaccc tcgccttcca gtcggcgctg ggcagcactg tctggctgat tgtcgcggcg
    80881 gtggtggcga tgtatatcgt gctcggcatt ctgtacgaga gctttattca cccgatcacc
    80941 attctctcga cgctacccac cgcaggggtc ggcgcgctgc tggcattaat gattgccggt
    81001 agcgaactgg atgtgattgc gattatcggc attattttgc tgatcggtat tgtgaagaag
    81061 aacgccatca tgatgatcga cttcgcgctg gctgctgagc gcgagcaagg catgtcgccg
    81121 cgcgatgcaa tctaccaggc ttgtctgttg cgttttcgtc cgatcctgat gaccaccctg
    81181 gcggctctgc ttggcgcgct gccgctgatg ttgagtaccg gggtcggcgc ggaactgcgt
    81241 cgtccgttag gtatcggcat ggtcggcggt ctgattgtca gccaggtgct gacgctgttc
    81301 accacgccgg tgatttatct gctgttcgac cgcctggcat tgtggaccaa aagtcgcttt
    81361 gctcgccatg aagaggaggc gtaagtgaag ttttttgccc tcttcattta ccgcccggtg
    81421 gcgacgattt tactgtcggt cgccattacc ctgtgcggca tactgggctt ccgtatgctg
    81481 ccggtcgccc cgctgccgca ggtcgatttt ccggtgatta tggtcagcgc ctcgctgccc
    81541 ggtgcgtcac cagaaacaat ggcgtcttcc gttgccacgc cgctggagcg ctcgcttggg
    81601 cgcattgccg gggtcagcga aatgacctcc agcagttcgc tcggcagcac acgtattatt
    81661 ttacagtttg attttgaccg ggacatcaac ggcgcagcgc gtgatgtgca ggcggcgatc
    81721 aacgctgcac aaagtttgct gcccagtggg atgcccagcc gtccgaccta tcgcaaagcg
    81781 aacccgtcgg atgcgccaat tatgatcctc acgctgacat ccgatactta ttcgcagggt
    81841 gaactgtacg atttcgcctc gacgcagctg gctccgacga tttcgcaaat cgacggtgtt
    81901 ggtgatgtcg atgtcggagg cagctcactg cccgccgtac gcgtcgggct gaatccgcag
    81961 gcgctgttta atcagggcgt gtcgctggac gacgtacgca ccgccatcag caatgccaac
    82021 gtgcgtaaac cgcagggcgc gctggaagat ggcactcacc gctggcagat ccagaccaat
    82081 gatgagctaa aaaccgccgc tgaatatcag ccgttgatta ttcactacaa caacggcggc
    82141 gcggttcgtc tgggcgatgt ggcgacggtg accgactcag tgcaggatgt gcgcaacgcc
    82201 gggatgacca acgccaaacc ggctatttta ctgatgatcc gcaaactgcc ggaagccaat
    82261 attatccaga cggttgacag catccgggca aaattaccgg agttgcagga aaccattccg
    82321 gcggcgattg atctgcaaat tgcccaggat cgctccccca ccattcgcgc ctcgctggaa
    82381 gaagtcgagc aaacgctgat tatctcggtg gcgctggtga ttctggtggt gtttttattc
    82441 ctgcgctcgg gtcgcgccac tattattccc gccgtttcgg tgccggtttc gctgattggt
    82501 acgtttgcgg cgatgtacct gtgcggattc agtctcaata acctttcgtt aatggcgctc
    82561 accatcgcta ctggtttcgt ggtggatgac gccatcgtgg tgctggaaaa cattgcacgt
    82621 catctggaag cgggaatgaa accgttgcaa gccgcactgc aaggtactcg cgaagtcggt
    82681 tttacggtgc tgtcgatgag tctgtcactg gtggcggtgt tcctgccgct gctgttgatg
    82741 ggcggattgc cgggccgact gttacgcgag tttgccgtga cgctttctgt cgccattggt
    82801 atttcgttgc tggtttctct gacattaacg ccaatgatgt gtggctggat gctgaaagcc
    82861 agcaagccgc gcgagcaaaa gcgactgcgt ggttttggtc gcatgttggt agccctgcaa
    82921 caaggctacg gcaagtcact gaaatgggtg ctcaatcata cccgtctggt gggcgtggtg
    82981 ctgcttggca ccattgcgct gaatatctgg ctgtatatct cgatcccgaa aaccttcttc
    83041 ccggagcagg acactggcgt gttgatgggc gggattcagg cggatcagag tatttcgttt
    83101 caggcgatgc gcggtaagtt gcaggatttc atgaaaatta tccgtgacga tccggcagtg
    83161 gataatgtca ccggctttac aggcggttcg cgagtgaaca gcgggatgat gtttatcacc
    83221 ctcaagccac gcgacgaacg cagcgaaacg gcgcagcaaa ttatcgaccg tctgcgcgta
    83281 aaactggcga aagaaccggg ggcgaatctg ttcctgatgg cggtacagga tattcgcgtt
    83341 ggtgggcgtc agtcgaacgc cagctaccag tacacgttgt tatccgacga cctggcggca
    83401 ctgcgcgaat gggagccgaa aatccgcaaa aaactggcga cgttgccgga actggcggac
    83461 gtgaactccg atcagcagga taacggcgcg gagatgaatc tggtttacga ccgcgacacc
    83521 atggcacggc tgggaatcga cgtgcaggcc gccaacagcc tgttaaataa cgccttcggt
    83581 cagcggcaaa tctcgaccat ttaccagccg atgaaccagt ataaagtggt gatggaagtg
    83641 gatccgcgct atacccagga catcagtgcg ctggaaaaaa tgttcgttat caataacgaa
    83701 ggcaaagcga tcccgctgtc gtatttcgct aaatggcaac cggcgaacgc tccgctgtcg
    83761 gtgaatcatc agggattatc ggcggcctcg accatctcgt tcaacctgcc gaccggaaaa
    83821 tcgctctcgg acgccagtgc ggcgatcgat cgcgcaatga cccagcttgg tgtgccttcg
    83881 acggtgcgcg gcagttttgc cggaacagcg caggtgttcc aggagacgat gaactcacag
    83941 gtgatcctga ttatcgccgc catcgccacg gtgtatatcg tgctgggtat cctttacgag
    84001 agttacgtac acccactgac gattctctcc accctgccct cggcgggcgt tggtgcgctg
    84061 ctggcgctgg agctgttcaa tgccccgttc agcctaatag ccctgatagg gatcatgcta
    84121 ttaatcggca tcgtaaagaa aaacgccatt atgatggtcg attttgcgct cgaagcccaa
    84181 cggcacggta acctgacgcc gcaggaagct attttccagg cctgtctgct gcgttttcgc
    84241 ccgattatga tgactaccct ggcggcgctg tttggcgcgc tgccgctggt gttgtcgggc
    84301 ggtgacggct cggagctgcg gcacctcctg gggatcacca ttgtcggtgg actggtaatg
    84361 agccagcttc tgacgctgta taccacgccg gtggtgtatc tctttttcga ccgtctgcgg
    84421 ctgcgttttt cgcgtaaacc taaacaaacg gtaaccgagt aaatgacaga acttcccgac
    84481 agcacccgct ggcaattgtg gattgtggct ttcggcttct ttatgcagtc gctggacacc
    84541 accatcgtta acaccgccct tccctcaatg gcgcaaagcc tcggggaaag tccgttgcat
    84601 atgcacatgg tcattgtctc ttatgtgctg accgtggcgg tgatgctgcc cgccagcggc
    84661 tggctggcgg acaaagtcgg cgtgcgcaat attttcttta ccgccatcgt gctgtttact
    84721 ctcggttcac tgttttgcgc gctttccggc acgctgaacg aactgttgct ggcacgcgcg
    84781 ttacagggcg ttggcggtgc gatgatggtg ccggtcggca ggttgacggt gatgaaaatc
    84841 gtaccgcgcg agcaatatat ggcggcgatg acctttgtca cgttacccgg tcaggtcggt
    84901 ccgctgctcg gtccggcgct cggcggtctg ctggtggagt acgcgtcgtg gcactggatc
    84961 tttttgatca acattccggt ggggattatc ggtgcgatcg ccacattgct gttaatgccg
    85021 aactacacca tgcagacgcg gcgctttgat ctttccggct ttttattgct ggcggttggc
    85081 atggctgtat taaccctggc gctggacggc agtaaaggta caggtttatc gccgctggcg
    85141 attgctggcc tggtcgcagt tggcgtggtg gcactggtgc tttatctgct gcacgccaga
    85201 aataacaacc gcgccctgtt cagtctgaaa ctgttccgca ctcgtacctt ttcgctgggc
    85261 ctggcgggga gctttgccgg acgtattggc agtggcatgt tgccctttat gacaccggtt
    85321 ttcctgcaaa ttggcctcgg tttctcgccg tttcatgccg gactgatgat gatcccgatg
    85381 gtacttggca gcatgggaat gaagcgaatt gtggttcagg tggtgaatcg ctttggttat
    85441 cgtcgggtac tggtagcgac cacgctgggt ctgtcgctga tcaccctgtt gtttatgact
    85501 accgctctgt tgggctggta ctacgttttg ccgttcgtcc tgtttttaca agggatggtc
    85561 aactcgaccc gtttctcctc catgaacacc ctgacgctga aagatctccc ggacaatctg
    85621 gcgagcagcg gcaacagtct gctgtcgatg attatgcaat tgtcgatgag tatcggcgtc
    85681 accatcgccg ggctgttgct gggacttttt ggttcgcagc atgtcagcgt cgacagcggc
    85741 accacacaaa ccgtctttat gtacacctgg cttagcatgg cgtttatcat cgcccttccg
    85801 gcgttcatct ttgccagagt gccgaacgat acgcatcaaa atgtagctat ttcgcggcga
    85861 aaaaggagcg cgcaatgaag ttctggcgac ctggtattac cggcaaactg tttctggcga
    85921 ttttcgccac ctgcattgtt ttgctgatca gtatgcactg ggcggtgcgt atcagttttg
    85981 agcgtggctt tattgattac atcaagcatg gtaatgaaca gcgattacaa ctgttaagtg
    86041 atgcgcttgg cgagcagtat gcgcagcatg gcaactggcg cttcctgcgc aacaatgatc
    86101 gctttgtctt tcagatcctg cgttcatttg aacacgataa ttcggaagat aaacccggcc
    86161 cgggtatgcc accgcacggc tggcgtaccc agttctgggt ggttgatcaa aacaacaaag
    86221 tgctggttgg tccgcgagcg ccgattccac ctgacggtac acggcgaccc attctggtca
    86281 acggtgcgga agttggcgcg gtgatcgcct cccccgttga gcggttaacg cgcaatactg
    86341 atatcaattt cgataaacaa cagcggcaaa ccagctggtt gattgtcgcc ctggcaacgt
    86401 tactcgcggc acttgccact tttctgctgg cgcgcggttt actggcaccg gtaaaacgac
    86461 ttgtcgatgg cacgcacaaa ctggcggcgg gcgatttcac tacccgcgta acgcccacca
    86521 gtgaagatga actgggcaaa ctggcgcaag acttcaacca gcttgccagc acactggaga
    86581 aaaaccagca aatgcggcgc gattttatgg ccgatatttc tcacgaactg cgtacgccat
    86641 tagcggtgct gcgcggtgaa ctggaagcta ttcaggatgg cgtgcgcaaa ttcacgccgg
    86701 agacggtggc gtctttacag gcggaggtcg gtacactgac caaactggtt gacgatctcc
    86761 atcagttgtc gatgtctgat gaaggcgccc tcgcctatca aaaagcaccg gtagatttga
    86821 tcccactgct ggaagtggcg ggcggcgcat ttcgcgaacg attcgccagc cgtggcctga
    86881 aactgcaatt ttccctgcca gacagtatta ccgtatttgg cgatcgcgac cgtttaatgc
    86941 agttattcaa taatttactt gaaaacagcc tgcgctacac tgacagcggc ggcagcctga
    87001 aaatctctgc cgagcagcac gacaaaacgg tgcgcctgac ctttgccgac agtgcgccag
    87061 gtgtcagtga cgagcagcta caaaaattat ttgaacgttt ttatcgcacc gaaggttccc
    87121 gcaaccgcgc cagcggtggt tccgggctgg ggctggcgat ttgcctgaac attgttgaag
    87181 cacataatgg ccgcattatt gctgcccatt cgccttttgg cggggtaagc attacagtag
    87241 agttaccgct ggaacgggat ttacagagag aagtatgacc gagttaccaa tcgacgaaaa
    87301 cacaccgcgt attttgatcg tggaagatga accgaagctg gggcagttgc tcattgatta
    87361 tctgcgtgct gcgagctatg cgccgacgct tatcagccac ggcgatcagg tactgccgta
    87421 tgtgcgccag acaccaccgg atctgatcct gttggatctg atgctccctg gcaccgatgg
    87481 cttgacgctg tgccgggaaa ttcgtcgttt ttctgacatt cctatcgtga tggtgacggc
    87541 aaaaatcgaa gagatcgacc gcctgctcgg actggagatt ggcgcagatg actacatctg
    87601 caagccgtac agcccacggg aagtggtagc gcgcgtcaaa accattttgc gccgttgcaa
    87661 accgcagcgc gaattgcagc aacaggatgc cgaaagcccg ttgattatcg acgaaggtcg
    87721 ttttcaggct tcatggcgcg gtaaaatgct tgacctgacg cctgcggaat ttcgtctgtt
    87781 gaaaacgctc tctcatgaac caggaaaagt gttctcccgc gagcaattgc tcaatcatct
    87841 ttatgacgac taccgcgtag taaccgaccg caccattgac agccacatta aaaacctgcg
    87901 ccgcaagctg gaatctctcg acgccgaaca gtcatttatc cgcgccgttt acggcgttgg
    87961 ttaccgctgg gaagccgacg cctgccgcat cgtttagttt tagcgacatg actttgttag
    88021 ccggagatgg cgttcagacc agatccggca acattatccc acgcatggtc agcaaactga
//
LOCUS       DADMKF010000002        74091 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.002, whole genome shotgun sequence.
ACCESSION   DADMKF010000002 DADMKF010000000
VERSION     DADMKF010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 74091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 74091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..74091
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.002"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(<1..1213)
                     /locus_tag="JNC24_000088"
     CDS             complement(<1..1213)
                     /locus_tag="JNC24_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element TnAs1 family transposase"
                     /protein_id="HAY9156952.1"
                     /translation="MPRRLILSATERDTLLALPESQDDLIRYYTFNDSDLSLIRQRRG
                     DANRLGFAVQLCLLRYPGYALGTDSELPEPVILWVAKQVQAEPASWAKYGERDVTRRE
                     HAQELRTYLQLAPFGLSDFRALVRELTELAQQTDKGLLLAGQALESLRQKRRILPALS
                     VIDRACSEAIARANRRVYRALVEPLTDSHRAKLDELLKLKAGSSITWLTWLRQAPLKP
                     NSRHMLEHIERLKTFQLVDLPEGLGRHIHQNRLLKLAREGGQMTPKDLGKFEPQRRYA
                     TLAAVVLESTATVIDELVDLHDRILVKLFSGAKHKHQQQFQKQGKAINDKVRLYSRIG
                     QALLEAKESGSDPYAAIEAVIPWDEFTESVSEAELLARPEGFDHLHLVGENFATLRRY
                     TPALLEVLELRA"
     gene            complement(1217..1776)
                     /locus_tag="JNC24_000089"
                     /pseudo
     CDS             complement(1217..1776)
                     /locus_tag="JNC24_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220837.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            1765..1932
                     /locus_tag="JNC24_000090"
     CDS             1765..1932
                     /locus_tag="JNC24_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9156953.1"
                     /translation="MPLHDVSLVEGGLSALSVPLCLKAHFCQAEIYNLRGHVSKNGKA
                     DSILTKRRGPA"
     gene            2086..3663
                     /locus_tag="JNC24_000091"
     CDS             2086..3663
                     /locus_tag="JNC24_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HAY9156954.1"
                     /translation="MRQNLPVTGRNLELPKDANILSTTSPQSHITYVNPDFIKISGFT
                     EEELLGQPHNIVRHPDMPPAAFEHMWSTLKSGRSWMGLVKNRCKNGDHYWVSAYVTPI
                     AKNGSIVEYQSVRTKPEPEQVLAAEKLYAQLRSGKAARPKLAASFSVKILLLIWGSII
                     SSAMAAGMLTDTSISSLLLATLMSGSLSSVSVLAILSPLGRLVERAKNISNNPLSQSL
                     YTGRTDEFGQIEFALRMMQAETGAIVGRIGDASNRLSEHTRGLLKDIESSNVLTVEQQ
                     AETDQIATAVNQMVASIQEVASNAQHAADAAGRADTETASGQRLVAHTSQSITALEGE
                     IRQATQVIHELEGQSNEISKVLDVIRGIAEQTNLLALNAAIEAARAGEQGRGFAVVAD
                     EVRSLAARTQQSTTDIQSMISALQERAQSAVTVMEQSSRQAHTSVAHAEEAATALDGI
                     GQRVNEITDMNAQIATAVEQQGAVSEDINRSIINIRDAADTNVQTGQNNLQSAKSVAQ
                     LTSALSELAKQFWEKRG"
     gene            complement(3811..4044)
                     /locus_tag="JNC24_000092"
     CDS             complement(3811..4044)
                     /locus_tag="JNC24_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAY9156955.1"
                     /translation="MMEIFWIMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLA
                     GQPYKGRNGRVEGTRELVIHGGTAEFGKNRTLS"
     gene            complement(4041..4310)
                     /locus_tag="JNC24_000093"
     CDS             complement(4041..4310)
                     /locus_tag="JNC24_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9156956.1"
                     /translation="MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQ
                     HNTWFRDQVAAGRQQLERGDVLPHDMVESSAAAWRDEMSRKVSDK"
     gene            complement(4365..4523)
                     /locus_tag="JNC24_000094"
     CDS             complement(4365..4523)
                     /locus_tag="JNC24_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001700046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9156957.1"
                     /translation="MAENGYGLAGLGMGKVKSINQYRLTPGFGGFTPVPYATTECRLP
                     CRWRGISW"
     gene            complement(5212..6081)
                     /gene="repA"
                     /locus_tag="JNC24_000095"
     CDS             complement(5212..6081)
                     /gene="repA"
                     /locus_tag="JNC24_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HAY9156958.1"
                     /translation="MNQTSFTESTYRQVKNPNPVFTPREDAGTLKFCEKLMEKAVGFT
                     SRFDFAAHVAYARSRGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIE
                     CGLATESEAGKLSITRATRALKFLSELGLITYQTEYDPTIGCNIPTDITFTPALFDSL
                     DISEEAVASARRSRVEWENRLRKKQGMDALGMDELIARAWRFVRERFRSYQAELKSHG
                     MKRARARRDAGRTRQDIVTLVKRQLTREIAEGRFRGSLEAVKREIDRRVKERMIMSRN
                     NNYTRLATASPLV"
     gene            6792..7433
                     /locus_tag="JNC24_000096"
     CDS             6792..7433
                     /locus_tag="JNC24_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000335849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusG"
                     /protein_id="HAY9156959.1"
                     /translation="MCLSELQARLNVGQPLFPTHALCIRRSQQHRSPPAGIDFRGAFR
                     ILNISELHQRNWYLAQYIPAGKNREHLFSWLSEQHVLPWTPLILKKVRRTDKVCGYRR
                     HIHAVFPGYFFLKADPESHSFTHLRRHSAFLDFVKMAGEIKTVREDIVQSLMKVYPDP
                     ALNPAAREELDAASTLWLTKARYQYLLRLDAQPLPESRIALLLHLVSDDGALT"
     gene            7574..8236
                     /locus_tag="JNC24_000097"
     CDS             7574..8236
                     /locus_tag="JNC24_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000154135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="traC"
                     /protein_id="HAY9156960.1"
                     /translation="MTILSQENPVILLLENMLEALSTAPDNINNERRRRRYLLNWLDT
                     ARQMREFRGMAEEFTTLRKLLATNSDIPVANTLKSLLESGNNAALYDLYRFRSALATC
                     RSLGWQIGTCAWNDQLLSETLSRAQTGKRHILQLNSTGDTFSTTGRMIRPVAFSLIHP
                     ATINTSEVEEIFRDEGFMLAHGRECSLNGSGRNMLSRILHVGHSGLAEEEWGMDNSLL
                     LH"
     gene            8503..9123
                     /locus_tag="JNC24_000098"
     CDS             8503..9123
                     /locus_tag="JNC24_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000176498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqiJ family protein"
                     /protein_id="HAY9156961.1"
                     /translation="MTLTLLTAYNAPYLAALTFVLLTGIAELIALLCGHSLSSAMDTP
                     DLPEGLTGEALDWLNIGRIPLLIVLCMLAGFFGISGILLQGLIIHLLQAPAPNILLAP
                     LCLLLTCPLVHRTGRLIAPWLPRDETTALTEEAFIGCMATITGYHAVAGSPCEGRFTD
                     RFGQVHYLLLEPEAGKEFRRGDKVIIVCRLSGSRWLGELNPWPDVL"
     gene            9150..10844
                     /locus_tag="JNC24_000099"
     CDS             9150..10844
                     /locus_tag="JNC24_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000482663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HAY9156962.1"
                     /translation="MFNLPGWLFTVIALVLTLFVIGLIFARLYRRASAEQAFVRTGLG
                     GQKVVMSGGAIVMPIFHETIPVNMNTLKLEVSRAAAESLITRDRMRVDVAVAFFLRVK
                     PSAEGISTAAQTLGQRTLTPEDLRSLVEDKFVDALRATAARMSMQDLQDARENFVQGV
                     QNTVAEDLSKNGLELESVSLTSFNQTARVHFNPDNAFDAEGLTLLTQETERRRRERNE
                     VEQDVEVAIREKNRDALSRRLEIEQQEAFMTLEQQQRVKTRTAEQSASIAAIEAERRR
                     EAESARILAERKIEEAEIERQQIVRTRQVEAEREVAIREIEQQQATEIASQARAIAVA
                     AKSEEQSQAEARASKALAEAVQAQQDVETTRQTAEADRTKQVALIAAAQEAETKAVEL
                     TVRAQAEKEAAELQAAAIIELAEATRQKGLAEAEAQRALNDAINVLSDEQTSLRFRLA
                     LLDALPAVIEKSTEPMKSIDGIKIIQVDGLNGGVTRGKTATTDISGGNLAEQAVTAAL
                     AYRTQAPLIDSLLKEVGLSGEGLTALTRPLVMPAPEKVPDVSEPVTAPDNSEPQQV"
     gene            10850..11167
                     /locus_tag="JNC24_000100"
     CDS             10850..11167
                     /locus_tag="JNC24_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001330559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilI type IV pilus biogenesis protein"
                     /protein_id="HAY9156963.1"
                     /translation="MPEFFRHLFHKECPHGIFWFKRGLKMSAEKFRVVIYTNTCEKKV
                     SYIPATEDINIAIMPFQTADNGLIIVCQNSRIIRRKERIPGSSCEWKDVTLAPKSRNR
                     VQL"
     gene            11283..11618
                     /locus_tag="JNC24_000101"
     CDS             11283..11618
                     /locus_tag="JNC24_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001402868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9156964.1"
                     /translation="MRTITTREQLLINGRVHERIATHIVTGAHGYETLCTNGYNVRYN
                     KAQQLVESCEKIAEGKLPVTCPVCFTIWQDVHRFTHVDFDTQSGKSDFIDTCHSEIIT
                     GHVSITGKK"
     gene            11845..12915
                     /locus_tag="JNC24_000102"
     CDS             11845..12915
                     /locus_tag="JNC24_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000730429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HAY9156965.1"
                     /translation="MKKKSLTLLLPSLLSGCTAFNATPVPDTTPPALVFVDGQIRESA
                     AIIAHTQKRLLPPPVTRPVTPPRPTTTATPTTAVSSQSTALPPRPLLQATQLPALVMT
                     GKPGQSSVVYLREAHQRSVSDWIRQLLPPGWQFRQQNAAIPALNKRLPVWSVNDQWHR
                     ALNALLAGQQLYGHMDWNRKILTVTTSATPPVDVTAPQGSQKAADTPRNPFRGSAATP
                     AGPTPVTPAVSVSRAQTTTGTSGTPESRTTTPAPVATKPVVPPQQKVIPPRQWKMEKG
                     TTLKDGLARWTSSEKCPHGTWTLRWETPVNYSIDAPLVFTGSFRQALEQLFALYTPAE
                     KPLYARTSTAQCLLRVTDRPQE"
     gene            12919..13356
                     /gene="pilM"
                     /locus_tag="JNC24_000103"
     CDS             12919..13356
                     /gene="pilM"
                     /locus_tag="JNC24_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilM"
                     /protein_id="HAY9156966.1"
                     /translation="MGWVTGFIMVIMAIGNFWFHSHLSRTVHHQQTAEITQQAADFIR
                     YMNAINDYLYQHPERRAAGGQLTSAQLGLPATKNVSHLISQQRVFVWAKEKPGLMGAL
                     LEQSGDSALLARVENGRLLDTRGRRISITLPAVIPDQVIIWMN"
     gene            13388..15007
                     /locus_tag="JNC24_000104"
     CDS             13388..15007
                     /locus_tag="JNC24_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN family type IVB pilus formation outer
                     membrane protein"
                     /protein_id="HAY9156967.1"
                     /translation="MNKKLLAIMIAATLLQGCTSFRQAENTRKDAVAEQQRAREMIAE
                     LRNRTPVVKESPRQWINPRPVTDKNPGNAVPGCPIIINTRQSITLQQVAQRISQNCHI
                     PVRITPDVQAYLSVTTSGSTQQIQGTVPPPDTGGMVPLAAIGSSDTTLPRLQGNTASL
                     PELKASGLTSLLNAVASRLGISWRYDGSHITFYYLDTRTFPVTYMDSQVAYNATVVSG
                     TMSSNGSSGGTASDAISGDASNTQTTTVDMKSALYQDVKNAVSSMLTPGIGRMFMSTG
                     FITVTDTPQVLETVRTFIEKRNEEMKRQVVLNVEILSIRKTRNEQAGIDWNAVFSDRT
                     LGLSLGSTFTSAASDTVTGGVSIVNGKLTGSKAFLKALSSQGDVSVVTRNSAVTKNLT
                     PVPMQIANQQSYIESVTTDTTANVGSSTSLNAATITTGFNMTLLPFILPDSQTLQLLY
                     SMSLSDKPVIENYESGGSKAQLPNVDLKTINQTVDLKSGQTVIISGFQQSGRRSGKQG
                     VGTPGFFGLGGGINSENDDTILVVLITPNII"
     gene            15028..16323
                     /gene="pilO2"
                     /locus_tag="JNC24_000105"
     CDS             15028..16323
                     /gene="pilO2"
                     /locus_tag="JNC24_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001597533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4b pilus protein PilO2"
                     /protein_id="HAY9156968.1"
                     /translation="MTDISPTERQVFPLTAGRNVFLAGMDWKTLPASHKNPRTFARSL
                     GAVRYISCEYLSTEDTDRHIMVAAVSQNTIPKGSRRYFSLAMLILPLLEPGGYAIVEL
                     SQANDTELYGFVSAVDGILVSDLVGTREEIREAREIFLTINSAPEHGWTCYEPPSFNG
                     PDGRGPLPLETLTGAGKYPAEARLHPVSRGPQLIPVLLILTLLGTAWYGWQYYERLKA
                     EKAALAEAAQKAAEERITPPWLSMPETGDFIRGCSDVWSRLPVSVAGWRYSLAECAAT
                     GNSGNLRASYTNIAGTTVTAFSQRVREIFSIRPFMVMPDGNSGGFALPVTFSMPETPV
                     AVEALPENTLLQERLTTLARSMQLKMDWQEVSNSFTDEDGNTIQPPWKEYDLQILTTL
                     PAHQVAEHFSEPSVRFISVTRQLEEGRFRYQFTGKYYVQ"
     gene            16313..16771
                     /gene="pilP"
                     /locus_tag="JNC24_000106"
     CDS             16313..16771
                     /gene="pilP"
                     /locus_tag="JNC24_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilP"
                     /protein_id="HAY9156969.1"
                     /translation="MSSKTKALLLSVLILPTTVAASTISNMQQNNRQLQVEQIQEETA
                     LYEAMLAREKVRHELRTLEQGEPAEPVHSQESSSAVTTDTPAPITGIPRLTEISGVHP
                     QITARIQLPGGQTADVKEGQHIPGTTFRVEKITTDSVVLEQNGIRTTLQP"
     gene            16875..18383
                     /gene="cpaF"
                     /locus_tag="JNC24_000107"
     CDS             16875..18383
                     /gene="cpaF"
                     /locus_tag="JNC24_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component"
                     /protein_id="HAY9156970.1"
                     /translation="MLAPADQPPDDILDYVVVDTDKTGQIHVRVVEGKKHLRAVQDYL
                     TRLCSRYPGKVSPFEFTTLDVIARLRQETVISGVENGINPVQQKMLGYISHAATLSAS
                     DLHITPGRDSTDFTYLEARVHGELELLDIVRKDEGLELLGATYSGMTDVIKGTQFDPG
                     VPQDARLAERFLKLAGLFGARYSHYPCVGGLYAVLRLIKDDSQHIPTFSMLGYHPEQE
                     RAVRRMLQRPEGIVILSGPTGSGKSTTLRTASAAYLEQYGFNDTGGILLPRRRLFTIE
                     SPPEGRIPGAIQTAVMDTAQGWVDSVKSALRLDPDAILNGEIRDHASAITAIKAAMTG
                     HLMLTTLHANDPINILERLEMEGVQARMIADPQLFIGLLSQRLVQVICPHCRLPWHEV
                     ESSRTDEERRLVENFCQPDAVYLRNHNGCPHCWRGVNGRTVIAEVISPDAKFFQIYRE
                     KGRIEAKTYWHRELGGMTRNQHLLGKINSGQVDPLAAHYISPVDEDSYTLLH"
     gene            18385..19479
                     /locus_tag="JNC24_000108"
     CDS             18385..19479
                     /locus_tag="JNC24_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion protein F"
                     /protein_id="HAY9156971.1"
                     /translation="MKILERLNNTELELSGLNRWLGKKTFRRTTFYESLAGMIRDGTP
                     VMRALEFICDVETDFGKKKGQSGLYFLATDCIASIRSSGQLSPALKDWVPKDEIALIR
                     NGEERGDIAEAMFQVVKTAKGRQEMISSLVSVCLYPLILLTLCVVNMYNVHTRVIPMI
                     SAFAPEERWSVQMVILAKSSGLVIGYGLWLLAGLLLLAILIRMSFSLYTGPRRRYLDR
                     IPPWSLYQTFHGVNYLFNISSMLQINIPLSHALTRIEKHAEHNRWLKSRISAIRKNVL
                     SGQSLALAMKNCGYDFPSKSCVNNLLLNSEREDSVASMALYADRWLEEAKKNVRKSGI
                     IITAISGLLVFLFIINMVSAIYGISDMLKQ"
     gene            19541..20077
                     /locus_tag="JNC24_000109"
     CDS             19541..20077
                     /locus_tag="JNC24_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003809694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HAY9156972.1"
                     /translation="MSVITSSYSEHNIRHLQNDDKGMTLLEVLGVLVVAAIVIGAVMG
                     LMSDTLSSSDNQKELKNLQTIATKMKAQKFQGQYTGTDYVKILTESGGLPADMIAGGN
                     KAKNAWGGAVIIKVSSDKYSYVIESSNVPKKNCIDLVTSLRSSSMFTKINGNVTNKVD
                     PSTVCNADKTTIKLETNS"
     gene            20122..20607
                     /locus_tag="JNC24_000110"
     CDS             20122..20607
                     /locus_tag="JNC24_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HAY9156973.1"
                     /translation="MNRLFILTVIFSLLCSLPSAQASPQDKQYDRCFITAGKRYSIDP
                     GLLKIIARRESSLNPRAINHNRNDRGKIISTDYGIMQINSTHIPGLKKMGIIRSEKDL
                     LNNVCLNIQTGAWILARHFQRCGVNWECLGSYNAGFSKSNTHRRMKYARQIYSAYMQG
                     R"
     gene            20623..21249
                     /locus_tag="JNC24_000111"
     CDS             20623..21249
                     /locus_tag="JNC24_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000939244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HAY9156974.1"
                     /translation="MLQIIPALLVAITVVIQCLAVQMSRYRLSRMNARPPHHGLALMS
                     GMLAAAAIILRLYQNDSAVGVLFMTIMTGCMTLMTLTDAASCRLPRPFTIMYLISGSA
                     FRLWQGDWLTGLCSAGFWFLLLLLFRQYTSYRRKTETIGLGDVFLIAGIAIWSPPGDI
                     PWIVATAAGGALLYLWCNRQRRITRELPFGPFLCASQYAFTFYPGGLW"
     gene            21267..22628
                     /gene="pilV"
                     /locus_tag="JNC24_000112"
     CDS             21267..22628
                     /gene="pilV"
                     /locus_tag="JNC24_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001168172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shufflon system plasmid conjugative transfer
                     pilus tip adhesin PilV"
                     /protein_id="HAY9156975.1"
                     /translation="MQKDNDKGFALLEILGGLVVISLLMPLFWSYIEDYLNEMRNQSA
                     AFHADAYNTAARTYIADNNARLHSGTLPATFTADELIRKGYLKGLNRSPFGQSYTTGI
                     RRNTSTGRLEALTCSTGGENIKDDALRSIASLLPGLGGFIGKNGTATGVFGGWTDKPG
                     DYGLSCNGGHIAIVMMGDDLQESDRLYRFQVPGRPELNQMNTAINMGGNNLNNAGNVN
                     GQSATLKGDVTSENGWLITKNDKGWKNITYGGGFTMTDSQWIRAVGGKGIITTGEIKG
                     GKVSGGTVRSDGRLSTGEYLQLDKTAVANTKCSPDGLVGRDSKGAILSCQSGVWVGFE
                     SYPVGSPIPWPSATPPQGYLLMNGQSFSCSRYPQLARAYPSCKLPDLRGVFIRGWDNG
                     KGLDGDRNRQLLSYQADQSGVYHERGGWLKGHHSGMPYWAQGSTTEMRPKNIAFNYIV
                     KAS"
     gene            23101..23922
                     /locus_tag="JNC24_000113"
     CDS             23101..23922
                     /locus_tag="JNC24_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HAY9156976.1"
                     /translation="MKTDSTGIAARMMLSLDRERICECLLSHRQLQSTPLQVRYPQGV
                     RDALGIMSEQLSLSVSDLTRILVEDALSEMFLPADNIVRRLLSRMEHIMQAHDISATT
                     MAALLAPWNIRPAVFREPDRLTDYLTGEILAALADWFYLSPEWLNGRVHYPLYRPGDW
                     PATQEIFCRIISARENMDIILWHGFPFAGTHSGEYCGVLLRQKKEINNTIIYPVLSLY
                     PARMDIEKEGWFQMARKISPDIPVRAVTLTPAQAEYLITGKILPTALFRVPLSPW"
     gene            24024..25226
                     /locus_tag="JNC24_000114"
     CDS             24024..25226
                     /locus_tag="JNC24_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
                     /protein_id="HAY9156977.1"
                     /translation="MMMKKHITRTLIASAVLFSFNSTAATSYFEARNDAMGGTGVASS
                     HYGVAPLANPALLTKSGAKDDFSLLLPSVGAQLSDPGNIADNADKISDDWKAFDRAVD
                     SHSGVPQTAARLKERLQDFRNTHASAQAGVSAVAALPGDTLAAALMLKTLGTVSVDGK
                     VSDADLNYLESIADSGSQDVDKNRLTSQAFARAALITDVGVALATELETAGQKWSLGF
                     TPKFQRVDLFNYNTLIKNYDSSAFKGDRYHNTQNGINADIGASMDLDDNWTLGLVAQN
                     LIPRSIETKEVHGVTETFRIRPQVTTGVSWHNDMFTTALDVDLTPASGFTSDSKRQFA
                     AVGAEFNAWKWAQLRTGYRQNLASNNGSAFTAGIGLSPFDVIHIDVAGLVGTDNNYGA
                     IAQLQFTF"
     gene            25330..25788
                     /locus_tag="JNC24_000115"
     CDS             25330..25788
                     /locus_tag="JNC24_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238171.1"
                     /note="Members of this family include DotD of type IVB
                     secretion systems and TraH of plasmid conjugative plasmid
                     systems, both lipoproteins; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotD/TraH family lipoprotein"
                     /protein_id="HAY9156978.1"
                     /translation="MQKKRITLAGGVAMLCLSGCHLPPDNTAPARKYGAASEVAVTRH
                     AQYQLWEQGTYSAALPSRQPGGSLQAHSGRVSFDWEGDALELLSELARLRGQQFSYSG
                     VRLPLPVSLHVRDMTFADALRLTEAQTAWRAVLSQSAGRLHLSYMPTEKK"
     gene            25785..26621
                     /locus_tag="JNC24_000116"
     CDS             25785..26621
                     /locus_tag="JNC24_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000745049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system DotC family protein"
                     /protein_id="HAY9156979.1"
                     /translation="MKKQVCLLLSGLLLAGCAGQHHRPPAGMAGNLPPPDLRAWLTPT
                     DNQDDGISDTRRQMLTDAGRTLGFRGGKAQRSWELIQALNARESTLNALYDFRPLISR
                     EGWLPPVIDEAQDVAHIQPDQIRTASRVWTILRPERFVSNPPGWRDWLLRGLSTTATP
                     GTEGRVVPEDRAQRRLWENALRQGWQEGRDNADLTLEANQKRLTRDYRGMMLYALLWR
                     QGMITRPDVTEQRQTVTGNGRKLITGDHVRRLKTHAEFTLQKSRWRPVVSTEGAPDEI
                     TR"
     gene            26605..27753
                     /gene="traJ"
                     /locus_tag="JNC24_000117"
     CDS             26605..27753
                     /gene="traJ"
                     /locus_tag="JNC24_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid transfer ATPase TraJ"
                     /protein_id="HAY9156980.1"
                     /translation="MKLPDEFGTFPFARFTAEEFRYFFAWCARHRVSDIDLTGGSPVS
                     VSRFGRRVRSSAHPLPSSIMMALSDELFGREVLPRVLSGHPVDRTIQVSGDASGRYGL
                     QRGQRVRLRCHLIQGTSATEEKVISVTMRVIPSDIPDILTMNIEPDLLDAMLRKTGLG
                     FICGETGSGKSTLAAALYRYIQTHFPDRKTVTYEDPVEYILGGENDLLPPHQAEIGRD
                     VESFAAGLRSAVRRNPDIIGIGEIRDYETADAAVQAGNTGHFCIGTMHTKSPGETFAR
                     LLGLFPPEIRDSMAAATLSLVQFILVQVLVRTNDGGRQAVREYIVITDELRDTLSRHS
                     HATWGHYIDEIIRKEKRRIRDQVLTMYQTGSIEASEAALFIPAGEFPK"
     gene            27750..28040
                     /locus_tag="JNC24_000118"
     CDS             27750..28040
                     /locus_tag="JNC24_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001578061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAY9156981.1"
                     /translation="MKREGFWRDVTPPLNIYGVPLPFTMIYLLLFPYPSKTTFWVCSG
                     ILLFFVLLGYSGWTVRTLCRRLYSLVQGKQASGRPWWYRHRTELPREWRGTE"
     gene            28104..32165
                     /locus_tag="JNC24_000119"
     CDS             28104..32165
                     /locus_tag="JNC24_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000842452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HAY9156982.1"
                     /translation="MKSRYRTWLAVPPEETEAVKNAVPPLNGRKAVAWDPEKKLWYAR
                     AGTELSLLERWLPRPQELSMDAGDPVTEFAQVLENAGLVIQGLPQMDGAIHRVTTRDD
                     KKGAKSGAYRAYLDGRPAGWYRDYRSADDSPTNWVFSGGEQHDPLARLHLRAFAQQQR
                     DDNARKLQQQYNKQAGYARSYINRLPQATAHEYLTRKGIRAAPGVRLNNKNELVIPFS
                     NGRGEIRSYQRIPVTGGKDARILKDSEKTGNWFTFGTPENGRPLLFAEGYATAASLHE
                     ATGLPVLMTVDASNMIAVAENARQIWTDSPFVFCADNDHQREINKGVFSATKAAEVTN
                     GEVIIPAFTEAEKAQGLTDFNDLDASRGRDNFQNAMNAQLKHIGIPTPNSDTADHREA
                     VVIDNLIFTPVKDEKPQMSPENRQSTAPETELATQDTPYDTHSDDTENQAYEDDYAAY
                     ASLQSVTAETLSPDKPLENIASAADTGTENTSVPTAGDQEDLNTDATGTDTAHNSDTA
                     ETGTLPEPAGMQSVQQATTEPTAEDKPHENVVSAADTREENASVATADTGEENASATT
                     ADNQSTQAEEHPRSKDTPETSLSDAAGTQHTTTTQSEEQVQVSMASADDWSAFAEDLN
                     ADTPGAPTVHNTDNAETNTVSEPAGMRPVQQVTTELTAEDKAQEKSVASAAETGAEHS
                     SDITDDNRNAPAEELNADATVAVHNTGTTETASPSEPAETQPVRQATAEPTAEDKLQE
                     ERLVSATETSTANEAARPETPSLFSRLRRFLTGVKSRTSEPSVIQEKKPTEAETATEE
                     VAPPSPPETSPEDTSSAEPDTIIFAPRRPDSPQRHNLDEILKNLTHKEFPDRTALYSV
                     DGEPAFLDRLYCLEMVDGASADDKKVLAALAVATNFYGGVIELTGSDAFKQKAMQLIV
                     EYDIKVRMKLPAQRAALEKIRKEKEMAHDAIVTHRPTPELNRQSPEETTMAQPAPPVS
                     PKAEAEQTAGSTAETPHPEAPVTAKAEAPRADRPEQPAAPSSAPEKAPVSAQPQQPDT
                     PEEEPPGKLSPGESVTAVLTNYGEKEYAPGKGRCFFVELTNRSGKREYWGNGLEELVK
                     NHQKGDPVTLTLQARETFRLSGETRDRIRNIWAITPVSNGITVNNDQPHAGQHTLEFP
                     MATFSQVMLQMQQHWPQIMQEVRSPKNPPNNLYIGEDRQPASAPQDQSMRLPLAGNAP
                     NELIPAIASADKDNRQLNLLLVHSADDHLQGVVRLNGTLYPALATPSADNRQLVINAL
                     TDNGLQFAGYGEAVNHDENNHQRPSPQIMQFHLKQQDSPLFAAIHKPEEQPDKLFRSL
                     GFEQTWKEWSDSQKAEDRQEKTLQQAQSHSPGR"
     gene            32182..32532
                     /locus_tag="JNC24_000120"
     CDS             32182..32532
                     /locus_tag="JNC24_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAY9156983.1"
                     /translation="MNMKKTLLVILTGITLASPVAGAATCRAGAAAEAGSRAAWERAR
                     TASQAWSQRENDVASSLQSCLSRIKDISITLPQFPSLDKLLRDLENQVCDAVVDRVND
                     HLPGNIDPWKDYNL"
     gene            32544..33239
                     /locus_tag="JNC24_000121"
     CDS             32544..33239
                     /locus_tag="JNC24_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraM"
                     /protein_id="HAY9156984.1"
                     /translation="MSEAQPTSPVSPEADNSGDIYNPVLRTIAQSEQRAALVPGLVRA
                     LLYSGAALCISLVANGIQYWHATGVTREYFATDNGRLVRLAPTSQPAWSQSDVMNFGS
                     MTLSEAFNLDFVHYRKQITTQAPRFSEAGFAGYVNALQASNILDTIKKEKFNLTTTIG
                     AGTLVNQGQMENGVWFWKFQYPVRMRLVGQTSTRPEQAFTFEITVRRVDPRIKPGGME
                     ISQMVSRNAAAGI"
     gene            33250..34224
                     /locus_tag="JNC24_000122"
     CDS             33250..34224
                     /locus_tag="JNC24_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001191879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraN"
                     /protein_id="HAY9156985.1"
                     /translation="MHIHHLIGALLIVCGTVTAADNPGWKNARSPQTGGKTAQATGNT
                     PAGGPPPAAQLPGNLPPPGAALVQEGASLDSPLNASEIRELRGIMADNERAISAPLTS
                     VVPRISSLTVNLSPGASLPLVRTALHNVSVVTFTDVNGSPWPQSDPPYNAAPGHFDVQ
                     FNGNMVSVTPLRAWAAGNVTVYLKGLSVPVILNITSGETDTTTLSQEIDSRLDLRIPR
                     RGPASPVVSIPSEKIALHDATLQAFLDGVPPDDPAIRRLKFSGNVPDTTIWQHGDDLL
                     VRSRAMLRDEFEQTLASADGTRLWKLPVTPLLTFSVNGQSVHVTPELE"
     gene            34228..35565
                     /gene="traO"
                     /locus_tag="JNC24_000123"
     CDS             34228..35565
                     /gene="traO"
                     /locus_tag="JNC24_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001272017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraO"
                     /protein_id="HAY9156986.1"
                     /translation="MSAEQEQDTAAKSGKKLSALLVMGGVIIIGGGYLLLTWLSPENA
                     ASLSSVNINSTTSGGGRNVMETQHYRDLLRADNERGAAEALRNNETFIASLPRGLDTD
                     TGNDQKRDNPPDDQSGTSGQNASPPQEDQTAREKRREALQKLLARINTQHQDARPPVV
                     AAAMWSTTGTPGTAPHASPSGTVTSQHPRLQETSLATPATRQGIQLIPALTRVPAYID
                     TEVDSDNAVSRVVASVPAGPWAGAVLYSQDARLAGQGMDIHFDRMMWRGMFLKVNAYA
                     LNERTGMSTVASDVNHRWFKHIVLPSVLGGVGNVGSLYKDANTQILQGNYGSITGRVG
                     MPDGEALAGVIAGGMAERGSQLLTRQAESEPYRQVTVSRGEVVSILFVEPVMSNDIST
                     PSSGTAPSESPSSGRPLTQAEAEHQASDRIRAAVLRKQNEMQQRYGIQQESYP"
     gene            35562..36275
                     /gene="traP"
                     /locus_tag="JNC24_000124"
     CDS             35562..36275
                     /gene="traP"
                     /locus_tag="JNC24_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraP"
                     /protein_id="HAY9156987.1"
                     /translation="MKNEHEDPGSFGETTAPASGKSFLKRQYMGYSLPVWAGCALIIL
                     VAIWYKFLRTPSEPPADAGFAVTPPAAESWAEPMTMTESGIGNNMPAPPVTPQADMQQ
                     LARDLKAELDRRDEEIRRTLTMLKDSITTLSNAIQKDEAWAQETRQLLEETRKQVHTL
                     QMQGALPAPTGNNRQPAKTLPSPLQGMKIMSVEKGMAWIRWQGSAWAVREGDSLGNIT
                     ITRIDPSSRAVITSAGTLR"
     gene            36275..36802
                     /gene="traQ"
                     /locus_tag="JNC24_000125"
     CDS             36275..36802
                     /gene="traQ"
                     /locus_tag="JNC24_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001388619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraQ"
                     /protein_id="HAY9156988.1"
                     /translation="MSIDALTAIENFASGIFSAGMEFLFTWGELLGIIGLIGHLMRAR
                     AEGRHSMGPGKFIAGIFICGMLVSLPSMINAGGTQMGFRADSFAPIAYVQPGSFGAAA
                     GAVNAILSLAKLAGVGFVMNGISIWRKSLLDGHTALSASESISKGNVKFVAGVLLVFN
                     DRVIDATLASLGIAF"
     gene            36849..37247
                     /locus_tag="JNC24_000126"
     CDS             36849..37247
                     /locus_tag="JNC24_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001605438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraR"
                     /protein_id="HAY9156989.1"
                     /translation="MSRLRAMAARLRAASVYVQLCLMACRDRLVRLLLLLPVMLLPHT
                     AMANTLFDMIKTVGDGARGSSSSFVAIGQVIGLVLFIGGLLGLKQIGKKPGAGLACLL
                     SILVGVCLAVVPELMGSGQKTLGLSPVSIS"
     gene            37304..37555
                     /locus_tag="JNC24_000127"
     CDS             37304..37555
                     /locus_tag="JNC24_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003665769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraS"
                     /protein_id="HAY9156990.1"
                     /translation="MSCELPPFTLLRTEHGNVIARDNRDNQEFVFAECYSTAIAALIL
                     AAVTLYTERKAYEKDPATHRPGPGTMAFPAPATGHGIQR"
     gene            37470..38288
                     /locus_tag="JNC24_000128"
     CDS             37470..38288
                     /locus_tag="JNC24_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001353682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraT"
                     /protein_id="HAY9156991.1"
                     /translation="MKKTPPLIAPVRERWLFPLPQPDTVFSGEALLPPPVLSTEGRCP
                     CCRRTVTHRFILEDSWPLQQMADTCRDTIVLLEKNLTRVMRLKKRPVPENADEKKKHT
                     RTLQDAERSLAQARLSARRLALRHVEKSQIVTTDALSENESDLLQPEGPPFHLCAFCH
                     AWHCLNGYAAAQGVMVWLPDLHPASVVALNARALQEIFSDNRQRVRQGRAVLNALVQN
                     RLAVEEKFRTWRPADFADALRRWPPAQRKTLREKMDGVALILLPDSFPDKKYVM"
     gene            38583..41627
                     /locus_tag="JNC24_000129"
     CDS             38583..41627
                     /locus_tag="JNC24_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAY9156992.1"
                     /translation="MNINRIIFAIEDCISTLSRYSVSAFFPDYCDLHTVIGLDQQDRE
                     RRPWLKAPYIATTKQGYYLSVFEISGALKEMDENPDQTAPGTLESLIAALAERMNTAW
                     KNSGHKISFVFERDPERGRDEIEAMLLPQRKSIARTGILLQDVLDEKVTTLTPWLVRE
                     RCWLTVWSSEELLSRTDRKNHQTRVRKLAEHAPPARFAQDPWRWTLSALKIRHDALLD
                     TLEQALTHDSDGLLIRLMDIHEVGREIRRQLERNSTPAVWQPHLPEDARPAGWRQGGD
                     TSVFHAPSLNLQLFTTQPETHGSLIQAGELWHGMVAITLPPQNLRTFNQLVRDVPRAI
                     PWRIRMDLMPDGMKALGVKKTLLTYSSFIPPLRPMYDSVMMLNDINKHDPVCIMTIVA
                     TTWGNSREVCTRNQALLKSALEGWGVCGTTTTFGDPRRAWVNTVLAASHSSGPIPLYP
                     PLSHALSLFPLNRAGSVWRGQGNLMMHTEDGSAWEVALASSQQNKHTELTPGAPGLGK
                     SVLINALSEIQIASAQKNLPFIAYIDKGFSAQGLVQLIRDSLPEQRKDEAVGIILSND
                     PDHTRNLFDVMYGARKPVTPEKNFMVSVLCALCVDTGTGQPCNPGDTRQIISSLVDLA
                     FREYGENNPRLYRAGTEPLVDLALEESGIAEQHDAGWWNAATWFEIRDMLHIAGNIPA
                     AQRAHYQAMPLLAEMSALLGQPSIRDVFGTVQRDNSEERLLDYIRRALDQGHSDYPMM
                     SGCTRFMLSPDTRVVAVDLNNVAGDKTPAGRLRTGIMYLLAGQIAGGDFVLPQYQEEI
                     RKNLDPRYHEVIFRRIEQLDQEVKTKVYDELHNAKGINFIWEALDTQELEQRKFGIRT
                     VLSTQLLQHYPPAVLKSANTLWLLRYRPDEIPFLRDNFGVPEVTLRRFLKMPEGAAPD
                     GSGVPVLAVFRVKNGTLARILKFTLGPLELWALNSSPKDSALRRALTQEVGSLRARQI
                     LAEHFPRGSATSLIEHRARTHDSENVIHELAAELIRKQGYNL"
     gene            41627..42247
                     /locus_tag="JNC24_000130"
     CDS             41627..42247
                     /locus_tag="JNC24_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraV"
                     /protein_id="HAY9156993.1"
                     /translation="MYSIHITPRCRRPARGQVFLKTLRVDGDSFSFTLPSACFTLRPS
                     PNPRVIFASDRRADGEMSGIRLALPGETGTLITTATWQVNVPERPPFDIQHVIHWLLP
                     APLTALMSDNTWLWPDVPGTPALTAGDWPVMESDTPTLKRVKRRTFTLIDNRNRQGWI
                     TRRFQCFPLAPLPESLSHRVPDILYPPKTEKKECKDNLQPHCLHSL"
     gene            42205..43410
                     /gene="traW"
                     /locus_tag="JNC24_000131"
     CDS             42205..43410
                     /gene="traW"
                     /locus_tag="JNC24_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraW"
                     /protein_id="HAY9156994.1"
                     /translation="MQRQSATTLLALLVGCAVIPATHAYSVNVVSSQPVTRQIMPELA
                     TIQGIMEEMVRMQTATGTAITQNSEKLATVIAQDGQATRQQMIFSNETHRLEEARKSF
                     SVPDSICSESASGIAAESRRAAASAAARLSQGGGVSSKPIRERLSRAADSPVREAYDS
                     AGIHAGYCTEAEYARFGGTDVCPAVGDLPGGDSQVRSLYQGAGTADTPAALTWDQKQI
                     DAATAYMKNTARPSAGRAPGKGEVGTQTGRTYVGLQNEYNGIIDAASHPQLSLIADST
                     PNEATRGALTEALQSPSAAAYFDRTASSEARTRGHMSQREFEAFEAGRRYANTDWQQD
                     LQGMEGDNLLRELLRTTALLNWQMNDLKEQIRQGNVIAGQQLALAARQYYGQRLGELS
                     QAMGQGSVR"
     gene            43407..43976
                     /locus_tag="JNC24_000132"
     CDS             43407..43976
                     /locus_tag="JNC24_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000650756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraX"
                     /protein_id="HAY9156995.1"
                     /translation="MKDDNSIDEKKPAPRLRRIGDIGINLFAPLAETRWMLRSCRHML
                     DQHKTRLKRLMPDRQPESPVCMTWEEAVRASGLTVDELDRRLRRRRMFWRTGFWLMMI
                     PVLLLCGMALAATALPVITLIRILVCLLLMSTIAALCASKALIASYRLWQLNERRVSE
                     DEQGTFPDYLQARHGWKQATWLAITHRQY"
     gene            44051..46216
                     /locus_tag="JNC24_000133"
     CDS             44051..46216
                     /locus_tag="JNC24_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotA/TraY family protein"
                     /protein_id="HAY9156996.1"
                     /translation="MKIIPRALSAGLALAAVPAQAFVTYQDIVSAATNPDDLSRQALV
                     TIFGDVVTNPLTASSNTIIGNLFMVFNAIIATMGIVWFLFIGIRHAVRTGHQGSVFNN
                     GRDVVGILSVVSGFLMIVPTASGWSLAQLVMLWGASIMGIGSANIMVQTAAENIAEGY
                     SMTVQPVHVSTRTAARGILEMELCKHAMNTGLNDFNRTARSSTPPMSESSRTENGRYT
                     VTVSNGSATCGSVSLSVKGNGSNKQSALAHFFNPFIETEYQGVLTAQRSAMDTMLRDL
                     DSAAQEFVSAYRTRRDTGNGNLPDIESRIQKAANRYEETVQKALPTDNAETERREQLK
                     SYLTTYGWVALGAWYQTFATANQRLAELADRAPTVSGVSSLGEAGDTEFLKEVKGAYQ
                     AQLQNSAYTPALGTTTSASEYHASNTQNPQSALLEFIGPGLTRFSNKVATIHSGTGTT
                     SQQANPLLKMKDIGDYTMVTTETIWAGYTTVRVLSTAGSNSVFGRLVNAVSGLSDAAK
                     AFLEASAPPLYFLLFLLFCAGFSLSIYLPFIPFIFWMTGIGNWIVSVLIGCAAGPLWA
                     ATHLGTSQDRGSRAAYGYIYLIDSMIRPLLMVFGFFFASVAIVAGGTILNALFAAALM
                     NVQINSVTGVFSMAGFLMIYARACTTLVAAIFALQAYLPDHVINFLGGRDGVNMLGNM
                     VSSVKDIIAGGNTNMRRTPGVREDRLKNINRDDKDGIKQ"
     gene            46305..46955
                     /gene="excA"
                     /locus_tag="JNC24_000134"
     CDS             46305..46955
                     /gene="excA"
                     /locus_tag="JNC24_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid IncI1-type surface exclusion protein
                     ExcA"
                     /protein_id="HAY9156997.1"
                     /translation="MNKTKSRYPSWWAYYHLARNAYFLLGIPCLLLYSAAYTSDLLDD
                     NLRHSISLFILAYGCYFLLFIPPLWLYLRTRAKKKKVQQVVQQIKESSPFAPTSDYEQ
                     LSFSKSCYFGIDIKNGTMLYVRIYPNNVMDVIGLDIHNFTRTVAEDGKLEIHTTYVSL
                     PMIPLEISGISARTLANTMHTMAARGYEYKERFPQMIRYRVKEWEKVAGVPVAEVF"
     gene            47229..48815
                     /locus_tag="JNC24_000135"
     CDS             47229..48815
                     /locus_tag="JNC24_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma"
                     /protein_id="HAY9156998.1"
                     /translation="MSESRSNFPARHGTPWNTEELQRLLVSIAEGVSLKKLTQTHQRS
                     SGGIRGAIKRLLPSEFLEFNDINTVNDLSNYFNKIPRQEHNSIINNLISLSTAKTIAH
                     DKVEIQKNSFKQNTPLPLCQPNTDFKDYIADEHDVIMLIHSAIESLSNKRHQYILLMR
                     LGTDDSPHSLADIAVKLGISRERVRQLQERALGLLAAKSRYEGTPGACLKALINSIGN
                     SPYDIAVWIYRIVHRDFVTSSELAAKFIIFAAGIPKARRDEIKNSLSLISQLQKQKLR
                     ERRAELAHAHAQERARIKKERVEFIFSKWLDNTHWPKYIAPPPPVEQLCSQRAVNDTD
                     ISGFFYSQKLGRTVQYESGLEFKIMNLLECCDQVLFYQEQPFSIPYCFKNKSKKYYPD
                     LLIATVDGRCLLMEVKPTDRMTLSLTRIKSNAGRKWAHTRGWGWLVISDRFSLRQLEE
                     HAISTNTWKLIEAELKTSRILAWREMMELRTRYEIHRLDLIAYIIQSGAEVDNFYRIT
                     PKISNNTSNQRQPNESDCHN"
     gene            complement(48953..49105)
                     /locus_tag="JNC24_000136"
     CDS             complement(48953..49105)
                     /locus_tag="JNC24_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001618166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HAY9156999.1"
                     /translation="MPQRTFLMMLIVVCVTILCFVWMVRDSLCGFRIEQGNTVLVATL
                     AYEVKR"
     gene            49405..49677
                     /locus_tag="JNC24_000137"
     CDS             49405..49677
                     /locus_tag="JNC24_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001305021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9157000.1"
                     /translation="MQNNKKQGHIMKDEIHSTSINPDVVEQFLALMNTHCKSSEEALA
                     MLSTLCYNLMMTCGIHSMDSQMLNGHGISMQLQGIDIAERPGKNIH"
     gene            49976..50344
                     /locus_tag="JNC24_000138"
     CDS             49976..50344
                     /locus_tag="JNC24_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001456887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9157001.1"
                     /translation="MKDNNTTATPEAWKMRFLANGTSGTWFYTDDIAVTDAWLRAHPD
                     DVEVIPLCRIQPRQHSHEQHIEELRAALARMLHMHHMMLKTTHYRENITDARCIREMH
                     EAPLQALNILSRYPHKKDEV"
     gene            50348..50563
                     /locus_tag="JNC24_000139"
     CDS             50348..50563
                     /locus_tag="JNC24_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001622039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9157002.1"
                     /translation="MWLLIFWLSSPFNVSSGDARQPTLAVHSQAFHTERACRDALRAI
                     KKENVGEFSLRGVCVSDGNTKEEAESS"
     gene            complement(50761..51384)
                     /locus_tag="JNC24_000140"
     CDS             complement(50761..51384)
                     /locus_tag="JNC24_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001579788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAY9157003.1"
                     /translation="MQEHIDYQIEKYQFRARNESPRLMRQWEDVMRECRDTGAGARER
                     LRIALLNVDYVTSFELPFRLLLTRTPQMIDSVRREWQLSQKKVVFNERQRGCVYSLQN
                     DLSGVPDSFSYSLATRIRRVDSYGMTTRCFTDIAGQVKAPTARLKLALESGLIVTALD
                     GLFWLGIQRIAADVQRLRQSGMTIITRDVEVSDSLTGTTRGVSGYSL"
     gene            51543..52010
                     /locus_tag="JNC24_000141"
     CDS             51543..52010
                     /locus_tag="JNC24_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000598960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HAY9157004.1"
                     /translation="MIKYIFSLTIIFSASLCAAQIQGHVIRVLDGDTLEILQDKKPVR
                     VRLANIDAPEKKQPFGRWSATQLKDLVAGKPVTVQYAQTDRYGRILGRVYTADGTEAN
                     RWQVRNGAAWVYPDYNNDYTLPPLQRDARALKRGLWADKNPVPPWTWRKQNKK"
     gene            complement(52401..52652)
                     /locus_tag="JNC24_000142"
     CDS             complement(52401..52652)
                     /locus_tag="JNC24_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9157005.1"
                     /translation="MNRLQVLAKTSGGAGFGALAGEELSITDTIENVATGNVRANHGV
                     LSPLMEKRMINLLIIVLRAVVAVANAVIALLELIRELVD"
     gene            53264..54286
                     /locus_tag="JNC24_000143"
     CDS             53264..54286
                     /locus_tag="JNC24_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS100 family transposase"
                     /protein_id="HAY9157006.1"
                     /translation="MVTFETVMEIKILHKQGMSSRAIARELGISRNTVKRYLQAKSEP
                     PKYTPRPAVASLLDEYRDYIRQRIADAHPYKIPATVIAREIRDQGYRGGMTILRAFIR
                     SLSVPQEQEPAVRFETEPGRQMQVDWGTMRNGRSPLHVFVAVLGYSRMLYIEFTDNMR
                     YDTLETCHRNAFRFFGGVPREVLYDNMKTVVLQRDAYQTGQHRFHPSLWQFGKEMGFS
                     PRLCRPFRAQTKGKVERMVQYTRNSFYIPLMTRLRPMGITVDVETANRHGLRWLHDVA
                     NQRKHETIQARPCDRWLEEQQSMLALPPEKKEYDVHLDENLVNFDKHPLHHPLSIYDS
                     FCRGVA"
     gene            54283..55065
                     /locus_tag="JNC24_000144"
     CDS             54283..55065
                     /locus_tag="JNC24_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAY9157007.1"
                     /translation="MMMELQHQRLMVLAGQLQLESLISAAPALSQQAVDQEWSYMDFL
                     EHLLHEEKLARHQRKQAMYTRMAAFPAVKTFEEYDFTFATGAPQKQLQSLRSLSFIER
                     NENIVLLGPSGVGKTHLAIAMGYEAVRAGIKVRFTTAADLLLQLSTAQRQGRYKTTLQ
                     RGVMAPRLLIIDEIGYLPFSQEEAKLFFQVIAKRYEKSAMILTSNLPFGQWDQTFAGD
                     AALTSAMLDRILHHSHVVQIKGESYRLRQKRKAGVIAEANPE"
     gene            55697..56995
                     /gene="trbA"
                     /locus_tag="JNC24_000145"
     CDS             55697..56995
                     /gene="trbA"
                     /locus_tag="JNC24_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TrbA"
                     /protein_id="HAY9157008.1"
                     /translation="MSYQQTSAAEDPMAIWYIVGAICLLFAIIIWRFLPEIVFASCLI
                     LHTLWGMIDWGPFHNFAAPRYNLLAITANNAATITFSQWLDVMSRTVGILWLILLPMT
                     FGFLWMWFHHPAQPRFTRRPLNIHTLPHLFSSLSPAIAPVLADGDSNRLFHGQKRPER
                     RVALTPEAFVEQNNLIRNMQLDVAATRQCFMAQLGQPLTTWKDMAPHEKALFAVFGLQ
                     FFLGDRKSAVSLMNNLNLSCRLKSKRDQGRFSTPVYSLARNAFIRVIKTEGAQQWLRQ
                     HRYVRSGLVWLYAHDLRLTPPNWLWLKGVDRTLFYALHRANTTKGFIEGAGVVAVARA
                     ENEASRLGLPCPEPCVEEAIEGLRRDMLGLGLIWDEPQPDRDRKRQIRTRWSLTDDVI
                     PRRHDNDEDTDTGETTETTETTETTETTEKQHPADKEKEQ"
     gene            56992..58116
                     /locus_tag="JNC24_000146"
     CDS             56992..58116
                     /locus_tag="JNC24_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HAY9157009.1"
                     /translation="MTIHYTATLNNKHLSLIALYPDGNREVIAILPVTELGALINRNN
                     RLMHLSFSSPFREITETVLVDTVDAERPGHHIGRMLSAIDACLDAEYAGKGKHPSRKN
                     GFWRTLMCGFIAGIALPATVAGYHWMKAEPPSTLAYPTSQEWRAEPVTDTETPRPHTE
                     HHVNSIPARPAPTQQEPSSAPAPTAVTEEQHVQAAEARRNLATVLKRNADRGMFTINL
                     STGHERTLYAFLDPACINCRLLEPALKQLAREFSVVIYPVSVIGGESSTKRTAPLLCE
                     PDSQKRATGWHQLYSADSGMAPTGDAPAAQDGQCLTAARAAIDVNNQAFRQFGFGGTP
                     WVLSDTGWHLPSGILKDATSVRLFLKATDSEEYHNEPAPR"
     gene            58097..60400
                     /locus_tag="JNC24_000147"
     CDS             58097..60400
                     /locus_tag="JNC24_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="HAY9157010.1"
                     /translation="MSQHHVNPDLIHRTAWGNPLWNALHNLNITGLCLAGSIITALIW
                     PLALPVCLLFTLVTSVIFMLQRWRCPLRMPMTLSLDDPSQDRKVRRSLFSFWPTLFQY
                     EADETFPARGIFYVGYRRINDIGRELWLSMDDLTRHVMFFATTGGGKTETTFAWLLNP
                     LCWGRGFTFVDGKAQNDTTRTIWYLSRRFGREDDIEVINFMNGGKSRSEIIQSGEKSR
                     PQSNTWNPFAFSTEAFTAETMQSMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVREGK
                     TMSLQMLREHMTLEGMAKLYCRGLDDQWPEEAIAPLRNYLQDVPGFDMSLVRTPSAWT
                     EEPRKQHAYLSGQFSETFTTFAETFGDVFAADAGDIDIRDSIHSDRILIVMIPALDTS
                     AHTTSALGRMFVTQQSMLLARDLGYRLEGTDAQTLEVKKYKGSFPYICILDEVGAYYT
                     DRIAVEATQVRSLEFSLIMTAQDQERIEGQTSATNTATLMQNAGTKFAGRIVSDDKTA
                     RTVKNAAGEEARARMGSLQRHDGVMGESWVDGNQITIQMESKIDVQDLIRLNAGEFFT
                     VFQGDVVPSASVYIPDSEKSCDSDPVVINRYISVEAPRLERLRRLVPRTVQRRLPTPE
                     HVSSIIGVLTAKPSRKRRKNRTEPYRIIDTFQRQLATAQTSLDLLPQYDTDIESRANE
                     LWKKAVHTINNTTREERRVCYITLNRPEEEHSGPEDIPSVKAILNTLLPLEMLLPVPD
                     LTASPPHKKNVAQTPSGGKQESRKKRF"
     gene            60879..61076
                     /locus_tag="JNC24_000148"
     CDS             60879..61076
                     /locus_tag="JNC24_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9157011.1"
                     /translation="MGSTKNGARVPHVRNRFTSMDIGMNSQIPLYCKQDIFIFYIQWL
                     IFLLIGNTMKNTMLRKGLTIF"
     gene            complement(61163..61240)
                     /locus_tag="JNC24_000149"
                     /pseudo
     CDS             complement(61163..61240)
                     /locus_tag="JNC24_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013221335.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            61264..62079
                     /gene="sul2"
                     /locus_tag="JNC24_000150"
     CDS             61264..62079
                     /gene="sul2"
                     /locus_tag="JNC24_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HAY9157012.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            62140..62943
                     /locus_tag="JNC24_000151"
                     /note="aph(3'')-Ib"
     CDS             62140..62943
                     /locus_tag="JNC24_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HAY9157013.1"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASR
                     RGELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAW
                     PSMGQQLGAVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLL
                     ARVERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLA
                     LMIANAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     gene            62943..63779
                     /locus_tag="JNC24_000152"
     CDS             62943..63779
                     /locus_tag="JNC24_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HAY9157014.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            complement(63751..64173)
                     /locus_tag="JNC24_000153"
     CDS             complement(63751..64173)
                     /locus_tag="JNC24_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005001938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9157015.1"
                     /translation="MNQHVWQEKELPGYVQREFEEFLQCGRLEHGFLRVRCESCHAEH
                     LVAFSCKRRGFCPSCGARRMAESAALLVDEVLPEQPMRQWVLSFPFQLRFLFGVVCGK
                     GRNPTLRLWPAIFSGEIDVFPGDRRSRLISSMTSVAPA"
     gene            complement(64385..64816)
                     /locus_tag="JNC24_000154"
     CDS             complement(64385..64816)
                     /locus_tag="JNC24_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tolA family protein"
                     /protein_id="HAY9157016.1"
                     /translation="MVLRSIWIEEQYMGVKKIVMLAILVSFVAGCAPLHPSNCHKTTA
                     LGSCSSGRWDDQDEWGEQARGIRDAINAKLDDPQKWKRKKCRLHMEFSRDGTALKIST
                     SNGDKAYCEAIKSAAHKAKFPAFNNPEVYRDFQKSGFDMRG"
     gene            complement(64884..67595)
                     /locus_tag="JNC24_000155"
     CDS             complement(64884..67595)
                     /locus_tag="JNC24_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAY9157017.1"
                     /translation="MNAIIPHKRRDGRSSFEDLIAYTSVRDDVQENELTEDSEIKSDV
                     PHRNRFRRLVDYATRLRNEKFVSLIDVMKDGSQWVNFYGVTCFHNCNSLETAAEEMQY
                     KADKAVFSRNDTDPVFHYILSWPAHESPRPEQLFDCVRHTLKSLELSKHQYVAAVHTD
                     TDNLHVHVAVNRVHPETGYINWLSWSQEKLSRACRELELKHGFAPDNGCFVHAPGNRI
                     VRKTALVRERRNAWRRGKKQTFREYIAQMSIAGLREEPAQDWLSLHKRLASDGLYITM
                     QEGELVVKDGWDRAREGVALSSFGPSWTAEKLGRKLGEYQPVPTDIFSQVGTPGRYDP
                     EAINVDIRPEKVAETESLKQYACRHFAERLPEMARNGELESCLDVHRTLAKAGLWMGI
                     QHGHLVLHDGFDKQQTPVRADSVWPLMTLDYMQDLDGGWQPVPKDIFTQVIPGERFRG
                     RNLGTQAVSDYEWYRIRMGTGPQGAIKRELFSDKESLWGYTTVQCESLIEDMIAGGNF
                     SWQACHEMFARKGLMLQKQHHGLVIVDAFNHELTPVKASSIHPDLTLSRAEPQAGPFE
                     IAGADIFERVKPECRYNPELAASDEVELGFRRDPVLRRERREARAAAREDLRARYLAW
                     KEHWRKPDLRYGERLREIHAACRRRKAYIRVQFRDPQLRKLHYHIAEVQRMQALIRLK
                     ESVKEERLSLIAEGKWYPLSYRQWVEQQAVQGDRAALSQLRGWDYRDRRKDKRRTTNA
                     DRCVILCEPGGTPLYEDTGVLEARLQKDGSVRFRDRRNGELVCVDYGDRVVFYHHQDR
                     NELVDKLNLIAPVLFDREPRMGFEPEGSYQQFNDVFAEMVAWHNAAGITGSGHFVISR
                     PDVDLHRQRSEQYYHEYIRQQKSISGGHGASYAPVQDNEWTPPSPGM"
     gene            complement(67607..67939)
                     /locus_tag="JNC24_000156"
     CDS             complement(67607..67939)
                     /locus_tag="JNC24_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nikA protein"
                     /protein_id="HAY9157018.1"
                     /translation="MSEKKTRTGSENRKRIVKFTARFTEDEAEIVREKAESSGQTVST
                     FIRSSSLDKVVNCRTDDRMIDEIMRLGRLQKHLFVEGKRTGDKEYAEVLVAITEYVNA
                     LRRDLMGR"
     gene            68172..68507
                     /locus_tag="JNC24_000157"
     CDS             68172..68507
                     /locus_tag="JNC24_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000157082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobC"
                     /protein_id="HAY9157019.1"
                     /translation="MASKKFYSDDDIQLAKAALSELPDLTAQRKTLRDFLDAIRDDII
                     ILVRTKGYTLTDVRDTLKNAGYEAGEKALRDIIREAESKKPSRRSSSKTAPKKTDSGR
                     KDGIDMNNN"
     gene            complement(68593..69441)
                     /locus_tag="JNC24_000158"
     CDS             complement(68593..69441)
                     /locus_tag="JNC24_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HAY9157020.1"
                     /translation="MNQTLPTADLNTAGTTDVIPSVAIDRIIAQRNEGIALFMQAMEC
                     LATARKILLDASGDIFLYGFEDCVTDSVRRMDKTEEAKRNITRLADRKIWDRLMTDTG
                     MYTFMSSCQRDEWNSQLMSDTCPEITLDNVLATFRHLNASKMQTFEQGLIDVYRKLSW
                     DYRTNNPCRLGKKIIIENLLYRWSNGRVTLDCSGREALDDLVRPFYLLEGRNVPDFRN
                     SIGAQYGEFLGNGDNVGKLLEGEYFTVRGYQKGTVHIVFKRSDLVEKLNDIIARHYPG
                     ALPPRV"
     gene            70021..70272
                     /locus_tag="JNC24_000159"
     CDS             70021..70272
                     /locus_tag="JNC24_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003980153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY9157021.1"
                     /translation="MTGWELRIWRKSMLWSREKAAREFGVTQRTWHAWENAEQVDVTV
                     WRTTQALSVRDLLPHMQGMRKADIIRRLENELGETAGNV"
     gene            complement(70303..70524)
                     /locus_tag="JNC24_000160"
     CDS             complement(70303..70524)
                     /locus_tag="JNC24_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001348086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9157022.1"
                     /translation="MPGFFVSRSRANSMTIAERLIQKGFDEGFKQGALEVAREIACRL
                     RDIGWPLERIQEVTGLSGEELKKLFPDEQ"
     gene            complement(70548..71474)
                     /locus_tag="JNC24_000161"
     CDS             complement(70548..71474)
                     /locus_tag="JNC24_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HAY9157023.1"
                     /translation="MSKKKNTTTPTPHDAAFRSFLANPDVARDFLELHLPAEYRQLCD
                     LSTLKLEPATFVEPDLHQYASDILWSVKTTGGEDGYVYTLIEHQSTENLYMPFRMLRY
                     SVAAMQRHLEQHKTLPLVIPVLFYHGERSPYPYSMNWLDCFENPALAAKIYTKPFPLV
                     DITVVDDNEIMNHRRMAALTLLMKHIRHRDMMELLDKLPQVMVEISDEQVRVLIHYIV
                     NAGDSVSPEFMRALAERLPQHEDKLMTIAERLEQKGRQEGRMEGRMEGRMEGALEKAL
                     AIACQLQKMGMTPEQIKQATGLSDDELKKITH"
     gene            complement(71471..71860)
                     /locus_tag="JNC24_000162"
     CDS             complement(71471..71860)
                     /locus_tag="JNC24_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein CbiX"
                     /protein_id="HAY9157024.1"
                     /translation="MKSCFDTDKAFHMSFDEVTFNADRIRSKLELLSEELMPRCGLVY
                     ELYQRRLSAAIDNFVATLPTEQHLLVLELAREEFDYLSAEEIAEEIRRDSERGYCCHG
                     FERNCCPLGCGDLGDDCCDAADLMEEP"
     gene            complement(72038..72304)
                     /locus_tag="JNC24_000163"
     CDS             complement(72038..72304)
                     /locus_tag="JNC24_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9157025.1"
                     /translation="MRPSIIFATAEYVKRLREECLRENKPLHRHTRFRRQELAPDEIN
                     PDVLAMGGHIARRCSEQKRVRIPAMKVSEWGHLLRALEIERVCH"
     gene            complement(72397..72831)
                     /locus_tag="JNC24_000164"
     CDS             complement(72397..72831)
                     /locus_tag="JNC24_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000218847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="post-segregation killing protein PndC"
                     /protein_id="HAY9157026.1"
                     /translation="MTVSSTISVFCRDGVFRTVYCHLHGEPTWNGRILHTHYATGQQA
                     EALVEHGDIRCLGPRCDKPAGHTLQNPVDGVTAYYGRDSGFRMDSEAREYRSFREAIA
                     TESTEEVRFHYVFIDGYWKVMYRTPEGWKMKALALALRRCPE"
     gene            complement(73564..74064)
                     /locus_tag="JNC24_000165"
     CDS             complement(73564..74064)
                     /locus_tag="JNC24_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAY9157027.1"
                     /translation="MSVVAPAVYVGTWHKYNCGSIAGRWFDLTTFDDERDFFAACRAL
                     HQDETDPELMFQDYEGFPGNMASECHINWAWVEGFRQARDEGCEEAYRLWVEDTGETD
                     FDTFRDAWWGEADSEEAFAVEFASDTGLLADVPETVALYFDYEAYARDLFLDSFTFID
                     GHVFRR"
BASE COUNT        18129 a        20675 c        18866 g        16421 t
ORIGIN      
        1 cggcgcgcag ttccagcacc tccagcaagg ccggcgtgta acggcgcagg gtggcgaagt
       61 tctcgccgac caggtgcagg tggtcgaagc cttccggccg ggccagcagc tcggcctcgc
      121 tgacgctctc ggtgaactcg tcccagggaa tcaccgcctc gatggcggca taggggtcgc
      181 tgccgctttc cttcgcttcc agcagcgcct ggccgatcct ggagtacagg cgcaccttgt
      241 cgttgatcgc cttgccctgc ttctggaact gctgctgatg cttgtgcttc gcgccgctga
      301 acagcttgac caggatgcgg tcatgcagat cgaccagctc atcgatcacg gtcgcggtgc
      361 tctccagcac cacggcggcc agggtcgcgt agcggcgctg cggctcgaac ttaccgaggt
      421 ctttgggcgt catctgccca ccctcgcggg ccagcttgag caggcggttc tggtggatgt
      481 gccggcccag gccttcgggc aagtccacca actgaaatgt cttcagccgc tcgatgtgtt
      541 ccagcatgtg ccgagagttg ggtttcagcg gtgcctggcg cagccaggtc aaccaggtga
      601 tgctgctgcc ggccttgagc ttcaacagct cgtccagctt ggcccgatgc gagtccgtga
      661 gtggttcgac cagggcgcgg tagacccgcc gattggctcg cgcaatggct tccgagcagg
      721 cccggtcaat cacgctcagc gccggcagga tgcgtcgttt ctgccgtagg ctctccaggg
      781 cctgaccggc cagcagcaag cctttgtcgg tctgctgggc cagctcggtt agctcgcgca
      841 ccagggcgcg gaagtcggac aggccgaacg gggccagttg caggtaggtg cgcagttcct
      901 gggcatgctc gcgacgggtc acgtcgcgct cgccgtactt tgcccagctc gccggctcgg
      961 cctggacttg cttcgccacc cacaggatga ccggctcggg cagctcgctg tcggttccca
     1021 gcgcgtaacc ggggtagcgc agcaggcaga gctgcacggc gaagccgagg cggttggcgt
     1081 cgccgcgtcg ctggcggatc agcgacaggt cggagtcgtt gaaggtgtag tagcggatca
     1141 ggtcatcctg gctttccggc agcgcaagca gggtgtcccg ctccgtggcc gagaggatca
     1201 agcgacgcgg catgtgtcag tcgtccgtgc ggaggtactg gtagagggtt tcccggctga
     1261 tgttgaactc gcgggcaagc tgcgccttgg gctcgccggc cgtcgctcgc tgccgcaggg
     1321 tagcagcctg ctcatcggac agggctttct tgcggccccg gtacgcgcca cgctgcttgg
     1381 ccaaggcgat gccctcacgc tgccgctcgc ggatcaggcg cgctcgaact cagcgaaggc
     1441 ccccatcacc gacagcatca ggttggccat cggcgagtcc tcgccagtga acaccaggcc
     1501 ctccttcagg aactcgatgc gcacgccgcg ctgagtcagc ttctgtacca agcgacgcag
     1561 gtcatcgagg ttgcgggcca gccgatccat gctgtgcacc accactgtat cgccttcgcg
     1621 gacgaagctc agcagcgctt cgagctgggg gcgctgggtg tccttgcccg atgccttgtc
     1681 ggtgaacacc ttgctcacct gggtttgttc cagctggcgt tccgggttct ggtcaaagct
     1741 gctgacccgg acgtagccga tgcggtgccc ctgcacgatg tctccttggt tgaaggcggc
     1801 ttaagtgcac tttctgttcc gttgtgcctc aaagcccatt tctgtcaggc tgaaatctat
     1861 aaccttcgcg ggcatgtgtc aaaaaatggg aaagcagact ctattctgac gaagcggcgc
     1921 ggccctgcct gacatcaagt tagggtatag cctagattga catgcgcgat gcaaccctta
     1981 acttgcttgc acctatcgtt tccatgctag ctttatcgta acgctcaaga aattgggctt
     2041 aatgccgaaa acaataaaat aaaacagcca acccttgagg ttcttatgcg ccagaattta
     2101 ccagtgacgg gtcgaaactt agaactccca aaagatgcca atattctttc gactacctcc
     2161 cctcaaagcc atatcacgta cgttaatcct gacttcatta aaatcagtgg tttcactgag
     2221 gaagaactat taggccagcc tcacaacatc gtaagacacc cagatatgcc gcctgctgca
     2281 tttgagcata tgtggagtac attaaaatct ggccgctcat ggatggggct agtaaaaaat
     2341 cgctgtaaaa atggcgacca ctattgggta agtgcttatg taacgccaat agctaagaat
     2401 ggttcgattg ttgaatacca gtctgtaagg accaagcctg aacctgagca ggttttggct
     2461 gcggaaaaat tatatgctca attgagaagc gggaaggccg cgaggccgaa attggctgct
     2521 agcttttccg tgaaaatact cttgctcata tggggtagta ttatatcaag cgcaatggct
     2581 gccggcatgc ttactgatac atcaataagc agcttattgt tagccacttt aatgtcagga
     2641 agcttaagct ctgttagtgt tttggctatt ctctctcctc ttggaagact ggttgaaaga
     2701 gccaagaata tttccaataa cccattaagt caatccctct acactgggcg caccgatgag
     2761 tttggccaaa tagagtttgc tttacgaatg atgcaagctg aaacaggcgc catagtaggt
     2821 cgcataggtg atgcatcaaa tcggcttagc gaacacaccc gaggcctact aaaggatatt
     2881 gagtcaagca atgtacttac agttgagcag caggcagaga cagatcaaat agcaacggca
     2941 gtaaaccaaa tggtggcaag cattcaagag gttgcgagca atgcacagca tgctgcagat
     3001 gcggccggaa gagcagacac tgagacggca tctggccagc gtctggtagc ccacacaagc
     3061 cagtcaatca ctgcccttga aggtgaaatt aggcaagcca ctcaggttat tcatgagctt
     3121 gaaggtcaaa gtaacgagat atcaaaagtt cttgacgtta tacgagggat cgccgagcaa
     3181 acgaatttgt tggcactcaa cgcagcaatt gaggccgcgc gtgctggtga gcaggggcgt
     3241 ggttttgctg ttgtcgccga tgaggttcgc agtcttgctg ctcgcacaca gcaatcgaca
     3301 acggatattc aaagcatgat cagcgctcta caagagcgag cgcaatccgc tgttacagtc
     3361 atggagcaaa gtagtcggca agcgcacacg agtgtagctc acgcagagga agcagctaca
     3421 gctcttgatg gaattggcca acgcgttaac gaaattaccg acatgaacgc gcaaatagcg
     3481 actgcggtcg agcagcaggg agcagtaagt gaagacataa accgcagtat tatcaatata
     3541 cgcgatgctg ctgataccaa tgtacagacc gggcagaata atttgcaaag tgcgaaatct
     3601 gtcgctcagt taactagcgc tctgagcgaa ctggcaaaac agttttggga aaaacgagga
     3661 taacgctttt cagtatctcg acagggataa cttactttac catcggttat cccttttagc
     3721 cgcacaaatt ttagccatgg cttttcagga agtcagggct atcagaatgg ccttagaaag
     3781 cctagtcaaa gagctgtcac gagaacaccg ttagcttagc gtacgatttt ttccgaattc
     3841 tgcggttccc ccgtgaataa ccagttcccg ggttccttcc acacgaccgt tgcgaccttt
     3901 atacggctgc ccggccaggc tgctggctga ataaccaatc cgttcgtcca gttcaatggc
     3961 ggccatcagg ttctgctccg caatgtactc acgaatgcgc ttcctgtcct ggctggccag
     4021 catgatccag aatatctcca tcatttatcg gaaaccttcc tgctcatttc atcccgccat
     4081 gcagcggcag aagattcgac catgtcatgg ggaagcacat cgccgcgctc gagttgctga
     4141 cgtcctgccg caacctggtc acggaaccag gtattatgct gccgttcaac ggtctggcgc
     4201 ataaaatccc gaattaactg agagccatta cggtccatgc tttttgcagc ggccataaaa
     4261 gcatccttca gttcagcgtc gattcttaaa ctcatattaa cctgtgccat ttatcacctc
     4321 gtcattacta cgtatataca cagtatataa cgattcagat atcgtcacca ggatatgccg
     4381 cgccagcggc atggaaggcg gcactccgtt gttgcatatg gcacaggagt aaaaccgccg
     4441 aagcccggcg taagccggta ctgattgata gatttcacct tacccatccc cagccctgct
     4501 aagccatacc cgttttcagc catgacagag cttctgtgcg cggtcggagt ggtcccgacg
     4561 agggtttacc cgaagtcggg gcgtatctcc gcgttagcgg gccgtcaggg ccgcttacga
     4621 gcgtgtactg aggacttcca gccagaagac tgacagcgat gaggattctt agttacaaca
     4681 ttcataatta aaagcgatgt tgttcccgcc cttcgggcgg gggcggcctc gcttttcagt
     4741 tatgaggggt aggcacggtg ctgcgtttgt gtggtgtttt tattcctgat gtctttttgt
     4801 tgtggtttca gaacggtgag gttcggggcc cgcgcgtcag cgtgggaggt gcatatttgt
     4861 ctgtccacag gacaggcact gaataggtct tttttttaaa tggaagtagt taagtagttt
     4921 aaaggaaata cagtactggg gtttaaaaga aacattgcac cctgtaacgc ctgcggctgg
     4981 cgctttatag cattaacggt tgtaacctta cggagaagtc ccttgcagtt aaatgtggat
     5041 aaacagaatt ccccgtctgt gagacgtgtt ttgcgtgaaa aacaggggga gcaaggcgag
     5101 ccggaaggca gatgatgagg ctgttttttg tgtgctgtgc tgcttttttg tgcgtcgccg
     5161 ggcttcagac gtgcggaagc ctccggcgca ggccggatta ttcagatgag gtcaaactaa
     5221 gggtgaggca gtggccagcc gtgtgtagtt gttgttccgc gacattatca tgcgttcctt
     5281 cacgcgacgg tcgatttcgc gcttcacggc ctccaggctt ccccggaaac gcccttccgc
     5341 gatttcacgt gtcagctgcc gtttcaccag ggtgacaatg tcctgacggg tcctgcctgc
     5401 atcgcggcgc gcacgggcac gtttcatgcc gtgggactta agctctgcct gataactgcg
     5461 gaaacgctcg cgcacaaaac gccaggccct cgctatcagt tcatccatac ccagcgcatc
     5521 catcccctgc tttttgcgca gcctgttttc ccattccacg cggctgcggc gtgcggaagc
     5581 cactgcctct tctgagatat cgagtgagtc aaacagtgcc ggcgtgaacg tgatgtcggt
     5641 cgggatgtta cagccaattg tcgggtcgta ttccgtctgg taggttatca gccccagctc
     5701 agacaggaac ttcagggcac gggtggcacg ggtgatggag agtttcccgg cctcagactc
     5761 cgtcgccagt ccgcactcaa tggccagcgt ggtgatggag cactgcacgc ggttggccag
     5821 cgggtcatag tggaaacaca gcccctgcag cagcgcatca atcgcccggc ggcgcagcac
     5881 cggcggcata cgccggcgca ggccgcgcga acgggcatac gccacatgcg cggcaaaatc
     5941 aaaacgggag gtgaagccca cggccttttc catcagtttt tcgcagaact tcagcgttcc
     6001 ggcatcttca cggggtgtga acaccggatt cgggttcttt acctgacggt atgtgctttc
     6061 agtaaatgag gtctggttca gggccgccac aagctgtcgc ttatggcggg aacagattgc
     6121 cgtgcgttaa tgcactgcaa agggttgaat ctctgacacg gtttcatata tacttatccc
     6181 gtattctgtg aggcccccat tatctttctc tgattccgcc aagtttcgag gaaagatgat
     6241 gggggttttc ctttatggtc ttctgaaact tttttcagcc ttccggcccc ggcaaactgc
     6301 cgtcgtttct gccatacatc ctgaatgcct tatggcgcat tctgccgttt tctggctttc
     6361 cggaccgcga ggatcccaaa agccactgca taacgatccg caatataccc cttttccacc
     6421 gctttgaaaa gccgacacga tttctcagag ttacagttgc cctctttaac cccctaaaaa
     6481 gcgtcgtttt tttgaactct gtcaaaaaat gaacaatttc acaaacgatc ttttatgctg
     6541 cttttttctt cttttccagt agaagagatc gccgaatttt tttaaacgat ctaatatccc
     6601 gcacacaaca tcaaatttag aataatattc tgtcttatca aatataacga tctattcaac
     6661 tgcttatcct cgctcttgcc acagatacat ccttatgtaa tatattttcc agccattaat
     6721 cctgtttgca ggagttgtcg gttttcgccc gcctcgaaac cgtccacaag gcttacaaga
     6781 gtgcgtaaat catgtgcctg tcagaactgc aggccaggct gaacgtgggt cagccgctgt
     6841 ttccgacaca tgctttatgt attcgccgtt cacaacaaca tcgaagcccc cctgcgggga
     6901 tcgattttcg cggagcgttc cggattttga atatcagtga attacatcaa cgcaactggt
     6961 atctggcaca gtacatccct gccggtaaaa accgcgaaca tctcttttcc tggctttccg
     7021 aacaacacgt ccttccctgg acacccctga tactgaaaaa ggtccgccgc acggataaag
     7081 tctgtggtta ccgtcgacat atacacgccg tgtttcccgg ttactttttc ctgaaagcag
     7141 accccgaaag ccacagcttc actcaccttc gccgtcacag tgcctttctg gacttcgtga
     7201 aaatggccgg tgaaatcaaa accgtcaggg aggatatcgt tcagtctttg atgaaagtgt
     7261 acccggatcc tgcgctcaat ccggcagcca gggaggagct ggatgctgcc tccaccctct
     7321 ggctgacaaa agcgcgttac cagtacctcc tccggcttga tgcccagcct ctgccggagt
     7381 cgaggatcgc cctgctcctg cacctggtat ctgatgacgg agcgttgaca tgaaaattgc
     7441 tgtcttaatt tgacgacttt tcccggcctt tatggccggg atttttttcc cttctgtttc
     7501 cacaatgccc ggcaccggtg acacctgagg gtgtacggag agcggctgtc gggcattttt
     7561 tcaggacgcc tttatgacca ttctatccca ggaaaaccct gtcattctgt tactggagaa
     7621 tatgctcgaa gcactctcca ccgccccgga caacataaac aacgaacggc gtcgccggcg
     7681 ttacctgctg aactggcttg acactgcccg tcagatgcgc gagttcagag gaatggctga
     7741 agagttcact accctgcgaa aactgctggc caccaattca gacataccgg tagcaaacac
     7801 cctgaaatca ctgctggaaa gtggcaataa cgcagcatta tacgacctgt accgcttccg
     7861 ctcggctctt gccacgtgca ggagtctggg atggcagatt ggcacgtgcg catggaatga
     7921 ccagttactg agtgagacac tttcccgcgc acagaccggg aaacgtcata tcctgcagct
     7981 gaacagcacc ggggacacct tttccaccac gggcagaatg atcagaccgg tggcattttc
     8041 attaatccat ccggccacca tcaacacgtc tgaggttgaa gagatattcc gggatgaagg
     8101 ttttatgctc gcacacgggc gcgaatgcag tctgaacggc agcggcagaa atatgctgtc
     8161 acgcattctt catgtgggcc attcaggttt agcggaagag gaatggggca tggacaacag
     8221 tctgctttta cactgaaata ccgggacagc cacaacggca gccactctcc gctgactttt
     8281 tttcgcaaat cgcggtttgc tgatgtggat gtcccatttt ttattatccg tcctctccgg
     8341 ttgtcataaa gcgcattccg gcgtgaggct tctgttgtta tcatcccctc ctgcagacat
     8401 ccccttgtct tctgatgact gacgatatgc ttcttctgac aattaaagaa gcggaagctt
     8461 cacgccataa tgttgtttga ctgacctgcc acaggaggaa aaatgacact gacgttactt
     8521 accgcgtata acgcacccta tctggcagcc ctgacttttg ttctgcttac tggtatcgct
     8581 gaacttatcg ccctgctctg cggacactcc ctctcttcag ctatggatac acctgattta
     8641 ccggagggtc tgacaggaga ggcgctggac tggctgaaca tcggacggat ccccctgctg
     8701 atcgtcctct gtatgctggc aggttttttt ggtatatccg gcattctcct gcagggtctt
     8761 attattcatc tgctgcaggc tcctgctccg aacattctcc ttgcaccact ttgcctgctg
     8821 ctgacatgtc ctctcgtaca ccgcaccggc agactgattg ccccctggtt accccgtgat
     8881 gaaacaacgg cgctcaccga ggaggcattc atcggctgta tggccaccat cactggttat
     8941 cacgcagtgg cgggttcccc ctgtgaaggt cgcttcacag accgcttcgg gcaggttcat
     9001 tacctgttgc tggagccgga ggccgggaaa gaattcagac gaggagacaa agtgattatt
     9061 gtctgccgtc tttccggttc ccgctggctg ggcgaactga atccctggcc ggatgttctt
     9121 taatcccgac atatacagag gttctcacta tgtttaatct tccgggatgg ttgtttaccg
     9181 ttatagcgct ggtcctcacc ctgttcgtta tcgggctgat atttgcccgc ctgtaccgcc
     9241 gggcatcggc agagcaggcc tttgtccgca caggtctggg aggccaaaaa gtggtaatga
     9301 gtggcggagc aattgttatg cccatttttc acgaaacaat tcccgtcaac atgaacaccc
     9361 tgaagctgga agtcagtcgt gcagcggctg aaagtctcat aacccgcgac cgcatgcgtg
     9421 ttgatgttgc cgttgccttt tttcttcggg taaaaccatc agccgaagga atatccactg
     9481 cagcccagac gctgggacaa cgcacgctca cacctgaaga tctgcgtagt ctggtcgagg
     9541 ataaatttgt tgatgcccta cgggccacgg cggccagaat gagcatgcag gatttacagg
     9601 atgcccgcga gaactttgtt cagggtgtgc agaacaccgt tgcggaagac ttatccaaaa
     9661 acggtctgga gcttgaaagt gtgtcgctga caagtttcaa ccagactgca cgggtacatt
     9721 ttaatcctga caatgccttt gatgctgaag gcctgaccct tctgacccag gaaacagaac
     9781 gtcgtcgccg cgaacgcaac gaagttgagc aggatgttga agtggccatc agggaaaaaa
     9841 accgggatgc cctctcccga cgtctggaga ttgaacagca ggaagccttc atgactctgg
     9901 agcaacaaca acgggtaaaa acccgtacgg cagagcagag tgccagtatt gctgctattg
     9961 aagctgaacg acgtcgtgaa gcagaatccg cgcgaattct ggctgaacgt aagatagagg
    10021 aagccgaaat cgaacgacag cagatagtgc gtacccgcca ggtcgaagcc gaacgggaag
    10081 tggccatacg ggaaattgag caacagcagg caaccgaaat cgccagccag gccagagcca
    10141 ttgccgtggc ggcaaaatcg gaagagcagt cacaggcaga agcccgggca agtaaagcac
    10201 tggctgaggc cgtacaggcc cagcaggatg tggaaaccac ccgccagact gcggaggccg
    10261 accgtaccaa acaggttgcc ctgattgctg ctgctcagga agctgaaacg aaagcggttg
    10321 aactgacggt tcgcgcacag gctgaaaaag aagcagccga actccaggca gcagcaatta
    10381 ttgaactggc ggaggccacc cgtcagaaag gtctggcaga agcagaagca cagcgcgcac
    10441 tgaacgatgc cattaacgtg ctttcggatg agcagaccag cctccgcttc cgtcttgccc
    10501 tgctggatgc attacctgcc gtcatagaga agtccacaga accaatgaaa tccattgacg
    10561 gtattaaaat cattcaggtg gatggtctga atggcggtgt tacccgcggt aaaacagcaa
    10621 caaccgacat aagtggcgga aatctcgctg aacaggcagt aaccgccgct ctggcttacc
    10681 gtacacaggc tccgctcatc gactccctgc tgaaagaggt gggtttatcc ggtgaaggac
    10741 ttacagccct gacacgtcca ctggtcatgc ctgccccgga aaaggttccg gatgtcagcg
    10801 aaccggtaac cgctccggat aactcagaac cacaacaggt ttaatccccg tgccggaatt
    10861 tttccggcat ctttttcaca aagaatgccc tcacgggata ttctggttta aaagaggatt
    10921 gaaaatgtcc gcagaaaaat tcagagttgt catatacacc aatacatgtg aaaagaaagt
    10981 cagttatatt cctgcaacag aagatataaa catcgctata atgccatttc agacggcaga
    11041 caatggctta attattgtgt gtcagaactc ccgtataata cgacgcaaag agcgtattcc
    11101 cggaagcagc tgtgaatgga aagatgtgac attagcacca aaatccagaa accgcgttca
    11161 gttatagcct gacagcctgc ttttcatccc ccataaaagg gggtaaaaat taaatatttc
    11221 acaagcgcga tatttttcag gaatgcagat tattttctgt tatgaccaat tgagaaacag
    11281 gcatgagaac aatcacaaca cgtgaacaat tattaattaa tgggagagtc cacgaaagaa
    11341 tcgccacaca tatagttaca ggagctcatg ggtatgaaac gctttgcaca aatggctata
    11401 acgtcagata caacaaggcg cagcaactgg ttgaaagctg cgaaaaaata gctgaaggta
    11461 aactacctgt tacctgtcct gtctgtttca ccatatggca ggatgtacac agattcaccc
    11521 acgttgactt tgacacacaa tcggggaaaa gtgattttat tgatacctgc cattcggaga
    11581 tcataaccgg acatgtttca ataacaggca agaaataaaa taatgttcca gttctgaaaa
    11641 acaatatcac ttgttttctg gaaactatca tttgcagcag gacgtttttc tttttatatt
    11701 tccggaaccg gaatgtttta gttttcataa tcaccccgaa catgaatgtt catcatcaat
    11761 atcctgatga aattatattt atctgaagac ctgttaccag ccacctgtct tataccgtaa
    11821 cccgatacat accggattta ttctatgaaa aagaaatcac ttacactcct tctgccgtca
    11881 ttactcagcg gctgtacggc atttaatgcc acgcctgttc ctgacaccac gcctccggct
    11941 ctggtgttcg tcgacggaca aattcgcgaa agcgctgcca ttatcgccca tacgcaaaaa
    12001 cgtctgcttc cgccaccggt gacccgccct gtgacgccgc cccgcccgac aacaacagcg
    12061 acgcccacaa ccgcagtcag cagccagagt accgcactgc ctccccgtcc gctccttcag
    12121 gccacacagc tgcccgcact ggttatgacc ggtaaacccg gtcagtcatc cgtcgtttat
    12181 ttacgtgagg cacatcagcg ctccgtcagt gactggatac gccagttgct accacccggc
    12241 tggcagttcc ggcaacagaa cgctgccata ccggcactga acaaacgcct ccccgtctgg
    12301 tccgttaatg accagtggca ccgggcactg aatgcgctgc tggccggcca gcagctttac
    12361 ggacatatgg actggaacag gaaaatactg actgtcacga cctctgcaac accgccggtc
    12421 gacgtcacgg caccgcaggg ttcacagaaa gctgctgata caccgcgtaa tccgttcagg
    12481 ggcagcgccg caacgccggc aggcccgaca ccagtgacac ctgccgtcag cgtttcccgg
    12541 gctcagacca ccaccgggac atccggaact ccagagagca gaacaaccac gccagccccg
    12601 gtggccacga aaccggtagt gccaccacag cagaaggtca taccgccacg gcagtggaaa
    12661 atggaaaagg ggaccaccct gaaagacgga ctggcccgct ggacatcatc cgaaaaatgc
    12721 ccgcacggca catggacact gcgctgggaa acacctgtca actacagcat cgatgcccct
    12781 ctggttttca ccggcagttt ccgccaggcg ctggagcagc tctttgcact ctataccccg
    12841 gctgaaaaac cgctgtatgc ccggaccagc actgcacaat gcctgctccg tgtgacagac
    12901 cgcccgcagg aataacctat gggatgggtt acgggtttca ttatggtcat tatggccatt
    12961 ggtaatttct ggttccacag tcacctcagc cgtactgtgc accatcagca gaccgctgaa
    13021 atcacacagc aggctgcaga ctttattcgt tatatgaatg ccatcaacga ttacctgtat
    13081 cagcaccctg aacgacgtgc tgccggtggt cagctcacgt cagcacagct cggtctgccc
    13141 gccacgaaaa acgtcagcca cctcatcagt cagcagcgcg tttttgtctg ggcaaaagaa
    13201 aaacccggcc ttatgggtgc cctcctggaa caaagtggtg attcggccct gcttgccaga
    13261 gtggaaaacg gtcgcctcct ggacacccgt gggcggcgta tttccatcac gctccctgct
    13321 gtgatccccg atcaggtcat catctggatg aattaggccg gactctgaat aactcacgga
    13381 tttatctatg aacaaaaaac ttcttgctat tatgattgca gccactctgc tgcagggctg
    13441 taccagtttc cgacaggcag agaacaccag aaaagacgcc gttgctgaac aacaacgggc
    13501 ccgggaaatg atcgctgagc tgcgaaaccg gacaccggtt gtaaaggaat ccccacgtca
    13561 gtggatcaat ccccgtccgg tcacggataa aaaccccggt aatgcggtgc ccggctgtcc
    13621 gattatcatt aacacccggc agagcatcac ccttcagcag gtggcccagc ggatctcgca
    13681 aaactgtcat attcctgtaa ggatcacccc tgatgtacag gcgtatctga gcgtgaccac
    13741 ctccggcagt acgcagcaaa tccagggcac agtaccaccg ccggataccg gcggtatggt
    13801 tccactggcc gccataggca gttctgacac gacacttccc cgcctgcagg gaaatacggc
    13861 gtcacttcct gaactgaagg cttcaggact gacatcactg ctgaatgcgg tcgcttcccg
    13921 tctcgggatt tcctggcgtt atgatggttc acatatcacg ttctattatc ttgatacccg
    13981 gaccttcccg gtgacctata tggacagcca ggttgcctat aatgccaccg tggtcagcgg
    14041 taccatgtca tcaaatggct catcaggtgg gacagcatcg gatgccattt ccggtgatgc
    14101 cagtaatacc cagaccacca ccgtggatat gaaatctgcg ctgtatcagg atgtgaaaaa
    14161 tgctgtctca tcaatgctga cgccgggtat tggtcgcatg ttcatgtcga cgggctttat
    14221 caccgtcacg gacaccccac aggtactgga aacggtccgg acgttcattg aaaaacgcaa
    14281 tgaagaaatg aagcgccagg tggtgctgaa tgtggaaatt ctgagtatcc ggaaaacccg
    14341 taatgagcag gcaggtatcg actggaacgc agtcttcagc gacagaacgc tgggactgag
    14401 cctcggcagc acattcacca gtgcagccag cgataccgtc actggtggtg tttccattgt
    14461 gaacgggaaa ctcaccggtt caaaagcctt cctgaaagca ctctcttccc agggagatgt
    14521 cagtgtggta acccggaatt ccgccgtgac caaaaacctg accccggtac cgatgcagat
    14581 tgccaatcag cagagctaca ttgagagtgt gacgacggat accaccgcca atgtcggctc
    14641 atccacctcc ctgaatgcgg ccaccatcac caccggtttt aatatgaccc ttctgccgtt
    14701 cattctcccg gactcacaga cactgcagct gctctattcc atgagtctct ccgacaaacc
    14761 ggttattgag aattacgaga gtggtggaag caaagcccag ctgccgaatg tggatctgaa
    14821 aaccatcaac cagacagtgg acctgaaatc cgggcagaca gtcattatca gtggcttcca
    14881 gcagtcaggg cgtcgcagtg gaaaacaggg tgtcggcaca ccgggattct tcggtcttgg
    14941 cggtgggatc aacagtgaaa acgacgacac cattctcgtg gtgctgatca cgcccaatat
    15001 catctgattt gcagaaggag gactatcgtg acagatattt caccgacaga gcgacaggtg
    15061 tttccgctga cggcaggacg gaatgtattt cttgccggaa tggactggaa aacactgccc
    15121 gcaagccata aaaacccccg gacgtttgcc cgcagtctgg gggcagtacg ctatatttcc
    15181 tgtgagtatc tcagtacgga agataccgat cgtcacatca tggttgcagc cgtcagccag
    15241 aatacaatcc ccaaaggcag ccgcaggtat ttctctctcg ccatgcttat cctgcctctg
    15301 ctcgagccag gaggatacgc tatcgttgaa ctgtcacagg caaacgatac agaactttat
    15361 ggttttgttt ctgctgttga cggcatcctg gtcagtgacc tggtgggaac ccgggaggaa
    15421 atcagggagg cccgggaaat attcctcacc attaacagcg cgcctgaaca cggctggaca
    15481 tgttatgaac cgccctcgtt taacgggcct gacggcagag gtcccctgcc actggaaacc
    15541 ctgacaggtg cagggaaata tccggcagaa gcacgccttc accccgtatc ccgtgggcca
    15601 cagcttattc cggtattact catactcacc cttctgggta ctgcatggta tggctggcag
    15661 tactatgaac gcctgaaggc agaaaaagca gcacttgctg aagcggcaca aaaagccgca
    15721 gaggaacgga tcacaccacc gtggctgagt atgccggaaa ccggagattt tatccgggga
    15781 tgcagtgacg tatggtcacg cctgccggtg tcggtcgccg gctggcgata ttcactcgcc
    15841 gagtgtgctg ccaccgggaa cagcggcaat ctgcgtgcat catatacaaa catcgccggg
    15901 acgacagtga ccgcattcag ccagcgggtc agggaaattt tcagcatccg gccgtttatg
    15961 gttatgccgg acgggaatag cggtggtttt gccctgccgg tcaccttcag tatgcctgaa
    16021 actcccgttg ccgttgaggc attaccggaa aacaccctcc tgcaggaacg gctgaccaca
    16081 ctcgcccggt cgatgcagct gaaaatggac tggcaggaag tcagcaacag cttcaccgat
    16141 gaagacggaa ataccatcca gcccccctgg aaggagtatg acctgcagat actgacgaca
    16201 cttccggcgc accaggtggc agagcatttc agtgaaccct ccgtccgttt tatctccgta
    16261 acaaggcaac tggaagaggg tcgtttccgt tatcaattca caggtaaata ttatgtccag
    16321 taaaacaaaa gctcttctgc tttccgtcct gatcctgccg acaacggtcg cggccagcac
    16381 catcagcaac atgcaacaga ataaccggca actccaggtg gaacagattc aggaagaaac
    16441 tgcactgtat gaagccatgc tggcaagaga aaaggtgcga catgaactca gaaccctgga
    16501 gcagggagaa ccagctgagc ctgtgcattc acaggaaagc agttcagccg tcacgacaga
    16561 tacccctgcc cccattaccg gtattccccg gctgacagag atatccggag ttcaccccca
    16621 gataaccgcc aggatacaac tgccgggcgg acaaactgct gatgtaaaag aagggcaaca
    16681 cattcccggc accacattcc gcgtggaaaa gatcaccact gacagtgttg tccttgaaca
    16741 aaatggtatc agaacaacac tacaaccctg actcagtgcc gccatttaca gccggctgtc
    16801 ggaatagatt attcccccgg cccttaatca caaaaaaaat cgtttccggg taccgctgcc
    16861 cgtaaggaga ttttatgctg gcacctgcag accagccacc tgatgacatt ctggattacg
    16921 tggttgttga tacagataaa acaggacaaa ttcatgtcag agtggtggaa ggaaaaaaac
    16981 accttcgtgc cgtccaggat tatctgaccc gcctgtgctc ccgctatccg ggtaaagtat
    17041 ccccgtttga gttcaccaca cttgatgtga tcgcccgact caggcaggag acggtcattt
    17101 ccggggtgga aaacggaatt aaccccgtac agcaaaaaat gctcgggtat atcagccatg
    17161 cggccaccct cagcgcctca gatcttcaca tcacgccggg gcgtgacagt acggatttca
    17221 cgtatctgga ggcccgtgtc cacggagagc tggagctgct cgatatcgtc agaaaagatg
    17281 aggggctgga attactgggg gccacctatt ccggaatgac tgacgtcata aagggaacac
    17341 agtttgatcc cggtgtcccc caggatgccc gtctggctga aagattcctg aaactggccg
    17401 gactgtttgg tgcgcgctac tcccactatc cgtgtgtggg cggactctat gccgtactgc
    17461 gtctgatcaa ggatgacagt cagcatatac cgacattcag tatgctgggg tatcaccctg
    17521 agcaggagcg cgctgtccga aggatgctgc agcgaccgga gggcatcgtg atcctctctg
    17581 gccccaccgg ttccggaaaa tccaccacgc tgaggacggc ttctgccgcc tatctggaac
    17641 agtatggctt caacgatacc ggagggatcc tgctcccccg gcgacgtctg ttcacaatag
    17701 aatcaccacc agaggggcgt atccccggtg caattcagac ggccgttatg gataccgctc
    17761 agggctgggt cgattctgtt aagtccgccc tgcgccttga tcctgacgct atcctgaacg
    17821 gggagatacg tgaccatgcc tccgccatca cggcgataaa agccgccatg accggtcacc
    17881 tgatgctgac cacccttcat gctaacgacc cgataaatat tcttgagcgt ctggaaatgg
    17941 agggcgttca ggcaaggatg attgcagatc cgcagttatt tatcggcctg ctcagccagc
    18001 gtctggttca ggtgatatgt ccccactgcc gcctcccctg gcatgaggtg gagagctccc
    18061 ggacggatga agaacgacgc ctggtggaaa acttctgcca gcccgatgcc gtgtatcttc
    18121 gtaaccataa cggctgccca cactgctggc gtggagtgaa cggccggacc gtcattgcag
    18181 aagtgatttc cccggatgcg aagtttttcc agatttaccg ggaaaaaggc cggattgagg
    18241 caaaaaccta ctggcaccgg gagctcggcg gtatgacacg aaatcagcac ctgctgggca
    18301 aaatcaacag tggtcaggtc gatccgctgg ctgcgcatta catatcccct gtagacgagg
    18361 acagctatac gctgctgcac tgatatgaaa attctggaaa gactcaacaa tacggaactg
    18421 gaactgtccg gccttaaccg ctggctgggc aaaaagacgt tcaggcggac tacgttttac
    18481 gagtcccttg caggcatgat ccgggacggc acaccggtta tgcgggcact ggagttcatc
    18541 tgcgatgtgg aaacggattt tggtaaaaag aaagggcagt ccggcctgta ttttctggcg
    18601 acagactgta tcgccagcat acgcagcagt ggtcagctct ctccggccct gaaggactgg
    18661 gtgcccaaag atgaaattgc cctgatccgt aacggtgaag aacgaggtga tatcgctgaa
    18721 gccatgtttc aggtcgtcaa aacggcaaaa ggacgacagg agatgattag cagtctggtg
    18781 agtgtctgcc tctatccgct catcctgctg acactctgtg tggtgaatat gtataacgtt
    18841 catacccggg ttattccgat gatcagcgcc tttgcaccgg aagaacgctg gtcagtacag
    18901 atggtcattc tggccaaatc ctccggactg gttatcggtt atggtttgtg gctgctggca
    18961 ggactgctgc tgctggcaat tctcatcagg atgtcattca gtctgtatac cggtccccga
    19021 cgacggtatc ttgatcgcat cccgccatgg tccctgtatc agactttcca tggcgtgaat
    19081 taccttttta atatttcatc catgctgcag ataaatattc ctttatctca tgcccttacc
    19141 agaatagaaa aacatgcaga acataaccga tggttaaaat cacgaataag tgctatcaga
    19201 aaaaatgtct tatccggtca gtcactggca ctggccatga aaaactgtgg ttatgacttt
    19261 ccgtcaaaat cctgtgtgaa taatcttctt ttaaattcag aaagggagga cagtgttgcc
    19321 agtatggcgc tttatgcaga ccgctggctg gaagaagcaa aaaagaatgt cagaaaaagc
    19381 ggcattatta ttactgctat atcaggtctg ctggtatttc tcttcatcat caatatggtc
    19441 agtgccattt acggcatcag tgacatgctg aagcagtaaa cacagcatcc tttatattcc
    19501 cctcgatcca aattctgttt taccaatctc aggagctaat atgagcgtca taacctcttc
    19561 gtattcagaa cataatattc gtcaccttca gaacgatgat aaaggcatga ccctgctgga
    19621 agttctcggt gttctggtcg tcgctgccat tgttattggt gccgtgatgg gactgatgtc
    19681 cgataccctg tcctcttcag acaaccagaa ggaactcaaa aacctgcaga caatcgccac
    19741 caaaatgaaa gcccaaaaat tccaggggca gtatacaggt actgactacg tcaaaatcct
    19801 gaccgaaagt ggtggcctgc ctgccgatat gatcgccggt ggtaataagg cgaaaaatgc
    19861 ctggggtggt gcggtaatca tcaaagtcag ttcagataaa tactcctacg ttattgaatc
    19921 cagcaatgtg ccgaagaaaa actgtattga tctggtcacc agcctgcgca gttcatccat
    19981 gtttacaaaa atcaacggca atgtgacgaa taaagttgac ccgtctacag tatgtaacgc
    20041 agataaaact actatcaaac tggaaaccaa cagttaatca ttcatcagcg cccgcccggg
    20101 cgctttattt aaggtcactg tatgaacagg ttatttatcc tgacagtgat tttttcactg
    20161 ttatgctcac ttccttccgc acaggcatcc ccacaggaca aacaatatga tcgttgtttt
    20221 attaccgccg gaaaacgtta ttccattgat ccaggattac tcaaaattat tgcccgcagg
    20281 gaaagcagtc tgaatccccg ggccattaat cataacagga atgacagagg aaaaattatc
    20341 agtaccgatt acgggataat gcagattaac tccacacata ttcccgggct gaaaaaaatg
    20401 ggcattatcc ggagtgagaa agatctgttg aataatgtct gcctgaatat tcagaccgga
    20461 gcatggatcc tcgcccggca ttttcagcga tgcggcgtaa actgggaatg cctcggcagt
    20521 tataacgccg gcttcagtaa aagtaacact caccgccgga tgaaatacgc ccggcagatt
    20581 tacagcgctt acatgcaggg acgttagtcc gggagaactg ccatgctcca gataatcccg
    20641 gcactgctgg tcgccataac cgtggtaata cagtgtctgg ccgttcagat gagtcgctat
    20701 cgactctccc ggatgaatgc ccgcccccct caccatgggc tggcgctgat gagcgggatg
    20761 cttgcagctg ccgccattat cctgcgtctt tatcagaatg acagcgctgt cggggtgctg
    20821 tttatgacga taatgaccgg atgtatgaca ctgatgaccc tgacggatgc tgcatcctgc
    20881 cgtcttcccc ggcccttcac catcatgtat ctgataagcg gcagcgcatt tcgtctctgg
    20941 cagggagact ggctgacagg cctgtgcagt gcagggttct ggttcctgct gttgcttctg
    21001 ttcaggcaat acacctctta ccgcaggaaa acagaaacca taggcctggg cgatgttttt
    21061 ctgattgccg gtattgccat ctggtccccg ccgggagaca tcccctggat tgtcgcaacg
    21121 gcagcaggcg gagccttact ttatctgtgg tgtaacagac aacgacgcat taccagagaa
    21181 cttccctttg ggccgtttct ctgcgcgtca cagtacgcat ttacttttta tcccggtggc
    21241 ctttggtaat accggaggga acgactatgc aaaaagacaa cgataaaggt tttgcacttc
    21301 ttgaaattct gggaggactg gttgtcatca gcctgctcat gccgctgttc tggtcttaca
    21361 ttgaagacta cctgaatgaa atgcgcaacc agagcgccgc atttcatgca gatgcctaca
    21421 atactgcagc caggacctac atcgccgata acaatgcccg cctgcatagc ggaacgcttc
    21481 ctgccacctt cacggcggat gagttaatcc ggaaaggata tctgaagggt cttaaccgct
    21541 cgccctttgg gcagagttat accaccggca tccgtcgtaa cacgagtacc ggtcgtctgg
    21601 aggccctgac ctgctccacc ggaggggaga atatcaaaga cgatgccctg cgcagtatcg
    21661 ccagcctgct ccccggtctg ggcggattta taggaaaaaa tggcacagca accggtgttt
    21721 ttggtggatg gacagataaa cccggcgact atggactcag ctgcaatggc gggcacatcg
    21781 ccatcgtaat gatgggtgat gacctgcagg aaagcgatcg cctgtatcgc tttcaggtac
    21841 cgggacgacc ggagctgaac cagatgaaca cggccatcaa tatgggaggg aacaacctga
    21901 acaatgcagg aaacgtcaat ggacaaagtg caacactgaa aggtgatgtt acaagcgaaa
    21961 acggctggtt aatcaccaaa aatgataaag gctggaagaa catcacctat ggtggcggtt
    22021 ttacgatgac agacagccag tggatacgcg cagtcggtgg taaaggaatt ataaccacag
    22081 gggaaatcaa aggaggaaaa gtcagtgggg ggacagtccg gtctgacggt cgcctttcca
    22141 caggtgagta tttacagctg gataaaacag ccgtagcaaa tacaaagtgt tcaccagatg
    22201 gacttgtagg ccgcgacagc aaaggggcaa tactttcgtg ccaatccggt gtgtgggttg
    22261 gctttgaatc ctaccctgtg ggaagcccta tcccgtggcc atctgccact cctccacaag
    22321 gctatctgtt gatgaacggc cagtcgtttt cctgttcccg gtatcctcag cttgcccgtg
    22381 cttatcctag ctgtaagtta cccgatctgc gcggtgtttt tatccgtggc tgggataacg
    22441 ggaagggact tgatggtgat cggaacagac agctcctgag ttatcaggcc gatcaatcag
    22501 gcgtctatca cgaacgtggc ggatggctga aagggcatca tagtggtatg ccatactggg
    22561 cacaggggag taccacagaa atgcgtccca aaaacattgc attcaactac attgttaaag
    22621 ccagctaggg actcacatct gataggtata cacagagaaa acaccgttac cacaggcgta
    22681 attttgtgtt ggtctggaga tgatttgata tgacgctccc actggcacgg caaatgaaat
    22741 aaatctccgg gcatatcagc tggtcagcaa actcgatgcc aggcgtaaac agaccagcaa
    22801 aatttcccct tattttgttc cctaccctgc aactgtcggg tgcagaaacg ggctgtttgt
    22861 cgtgacactg cacgatttcg accttgaaac ccgtgcagaa acccggggac tggcaatatc
    22921 tcatgccagc atgctcagtc acctcctgtc cgaatccttt tcattcgccc ttaaaacaga
    22981 gttgcaaaca ctcttgaaac acactcgatc ataacgatcg aaataactcg cttccgttta
    23041 aaaaacactc tctttagact caatttatga acaaaacacg ctgacccgca tggaggtgaa
    23101 atgaaaactg acagcacagg tattgccgcc cgtatgatgc tctccctgga cagggagcga
    23161 atttgcgaat gcctgctcag tcatcgccag ctgcagtcca cacccctgca ggttcgttat
    23221 ccgcagggag tcagggatgc ccttggtatc atgagcgaac agctttctct ttccgtctct
    23281 gacctgacgc gtatcctggt cgaagacgcg ttaagtgaaa tgtttcttcc tgcagataac
    23341 atcgtccgcc gtcttctcag caggatggag cacatcatgc aggcacatga tatcagcgca
    23401 acaacgatgg ctgcattact ggccccctgg aatatccgcc ctgccgtctt ccgggagccg
    23461 gacagactca ctgactatct gaccggggag attcttgcag cactggctga ctggttttat
    23521 ctcagtccgg aatggctgaa cggcagggtg cattatccac tgtatcgtcc gggagactgg
    23581 ccagccacac aggagatatt ctgcaggatt atttcagccc gcgagaatat ggacattatt
    23641 ctctggcatg gttttccgtt tgccggaacg cattccggag aatattgcgg cgttctgctc
    23701 aggcagaaaa aagaaattaa taacaccatt atttatccgg tactttcgtt atatccggcc
    23761 agaatggata tagaaaaaga aggctggttt cagatggccc gaaaaatatc tccggatatc
    23821 cctgttcgcg ccgtaacact cactccggca caggcagaat atctgataac aggaaaaata
    23881 ttacccactg cgttattcag ggttccgtta tccccctggt aataatactg agcaataaac
    23941 cggaataata caccaactgt ttttaatggc acagccgtgc cctctgttgt tatacccaaa
    24001 atacctcaat acaggaaact gaaatgatga tgaaaaagca tattacccgt actctcatcg
    24061 cgtctgccgt tttattttcc tttaattcga ctgcagcaac gtcatatttt gaagcccgca
    24121 atgatgcgat gggaggaacg ggggttgcct cctctcacta tggtgtggcc ccactggcaa
    24181 acccggcact gctgacaaaa tccggtgcaa aagatgactt cagtcttctg cttccgtccg
    24241 tgggtgcgca gctgtccgat ccgggtaata tcgccgacaa cgccgacaaa atcagcgatg
    24301 actggaaagc cttcgaccgt gccgtcgaca gccactccgg ggtaccgcag acagcggccc
    24361 gcctgaaaga gagactgcag gacttccgga acacccatgc cagcgcacag gccggggtat
    24421 ctgctgtggc ggcccttccc ggtgataccc tcgctgctgc actgatgctg aaaacgctcg
    24481 ggactgtcag cgtcgacggt aaagtcagcg atgccgacct gaactatctg gaaagcattg
    24541 cagacagtgg cagccaggat gtggataaaa acagactgac ctcacaggct tttgcccgtg
    24601 ccgcactgat caccgatgtg ggcgttgcgc tggccactga actggaaacg gccggacaaa
    24661 aatggtcact gggcttcaca ccaaaattcc agcgtgttga cctgtttaac tacaatacgc
    24721 tgatcaaaaa ctatgacagc agcgcattca aaggtgaccg ttaccacaac acccaaaacg
    24781 gtattaacgc tgatatcggt gccagcatgg acctggatga caactggacg ctgggtctgg
    24841 tcgcacagaa cctgatccca cgcagcattg agaccaaaga ggtacatggt gtcactgaaa
    24901 ccttcaggat ccgtccgcaa gtaacaaccg gcgtatcatg gcacaacgac atgttcacca
    24961 cggcactgga tgtggatctg accccggcca gcggcttcac atcagacagc aaacgtcagt
    25021 ttgccgccgt aggtgctgag tttaatgcct ggaaatgggc acagttacgt accggttacc
    25081 gccagaatct ggccagcaac aacggcagtg cattcactgc cgggattggg ctctcaccgt
    25141 ttgatgtgat tcacattgat gtcgccggcc tcgtgggcac cgacaacaac tacggtgcca
    25201 ttgctcagtt acagttcacc ttctgattat cacgccccgg gacgcccggg gcttctgacc
    25261 tgaatctgct cgtttccgct cttctggcca ctcaccgcca gtcctgttca ttattttccg
    25321 gataaaacca tgcaaaaaaa acgtatcacc cttgcagggg gagttgccat gctctgtctg
    25381 agcggctgtc atcttccccc ggataatacc gcaccggcca ggaagtacgg tgcagcttct
    25441 gaagtcgctg tcacacgaca tgcacagtat cagttgtggg agcagggcac gtattctgca
    25501 gccctgcctt cccgccagcc cggaggcagc ctgcaggctc acagcgggcg ggtttccttt
    25561 gactgggaag gcgatgctct ggagctcctg agcgagctgg cccgtctgcg cggacaacag
    25621 ttcagttaca gcggcgtgcg tcttcccctg cccgtctcac tccatgtacg cgatatgacc
    25681 ttcgctgatg cccttcgtct cacagaggcc cagactgcct ggcgggcagt cctctcacag
    25741 tcagccgggc gtttacatct ttcctatatg ccaacggaga aaaaatgaaa aaacaggtct
    25801 gtctcctgct gtccgggctc ctgctggcag gctgtgccgg acagcaccat cgcccccctg
    25861 ccggtatggc aggcaattta ccaccgccgg atctccgggc atggctgacg cccactgaca
    25921 accaggatga cggcatttca gacacccgtc gccagatgct cacggacgcc ggacgaacgc
    25981 tgggattccg tggcggaaaa gcacagcgct catgggaact gattcaggca ctgaatgccc
    26041 gggaaagtac actgaatgcc ctgtatgact tccgtcccct tatcagcagg gaaggctggc
    26101 taccccctgt tattgatgaa gcgcaggatg ttgctcacat tcagcctgac cagataagaa
    26161 ccgcgtcccg tgtctggacc atcctgcgcc ccgaacgttt tgtcagtaac ccgccgggct
    26221 ggcgtgactg gcttctgcgg gggctttcca ctaccgccac cccgggaact gagggccgtg
    26281 ttgtaccgga agaccgcgca cagcgcagac tctgggaaaa cgcactccgc cagggctggc
    26341 aggaaggccg tgacaacgct gacctcaccc tggaagccaa ccagaaacgc ctgacccggg
    26401 attaccgggg aatgatgctt tatgccctgc tctggcgtca gggcatgatc acacgcccgg
    26461 atgtgactga acagcggcag accgtcaccg gtaacggcag gaaactcatc accggagacc
    26521 acgtccgccg tctgaagaca cacgcggaat tcaccctgca gaaatcacgc tggcgtccgg
    26581 tcgtcagcac ggaaggagcc cccgatgaaa ttacccgatg agttcggcac attccctttc
    26641 gcccgtttca cggccgaaga gtttcgctat ttttttgcct ggtgtgcccg acaccgcgtc
    26701 agtgatatcg atctgacagg cggctcaccg gtatctgtca gtcgctttgg acggcgggta
    26761 cgttcgtccg cacatcctct gccgtcctcc atcatgatgg cgctgagtga tgaattgttt
    26821 ggacgggaag tccttccccg ggtgctgtcc ggtcatcccg tggaccgtac cattcaggtc
    26881 agcggtgatg cctccggtcg ctacggtctg caacgggggc agcgtgttcg cctgcgctgt
    26941 catctgattc agggaacatc agcaacagaa gaaaaagtaa tctccgtcac catgcgggtg
    27001 atcccgtccg atattccgga catcctcacc atgaatattg aaccggacct gctggatgcc
    27061 atgctccgga aaaccggtct gggtttcatc tgcggagaga ccggctccgg taaatccaca
    27121 ctggcagcgg cactgtaccg ctacattcag acgcatttcc ccgaccgcaa aacggtgacc
    27181 tatgaagatc cggtggaata cattctgggg ggagagaatg accttctgcc accccatcag
    27241 gccgaaatcg ggcgtgatgt ggaaagtttt gcggcaggac ttcgctctgc cgttcgccgt
    27301 aacccggaca tcatcggtat cggtgaaatc agggattatg aaacggcaga tgcagccgtc
    27361 caggcaggta acaccggtca cttctgcatc ggcaccatgc acaccaaatc acccggtgaa
    27421 acctttgccc ggctgctggg gttatttcca ccggaaatca gggattcaat ggcagccgcc
    27481 accctttccc tggtgcagtt cattctggtg caggtcctgg tcagaacaaa tgacggtggc
    27541 agacaggccg tccgggaata catcgtcata actgacgaac tgcgtgatac gctctcccgg
    27601 cactcccatg cgacatgggg gcattacatt gatgaaatca ttcgcaagga aaaacggcgg
    27661 atacgggatc aggttctgac catgtatcag acgggcagca ttgaggcatc agaagctgcg
    27721 ctcttcatcc cggccggaga attccccaaa tgaaacgcga aggcttctgg cgggatgtca
    27781 ctccgccact gaatatttat ggtgtcccgt tgccgtttac catgatttac ctgctgctgt
    27841 ttccgtatcc gtcaaaaaca acattctggg tgtgcagcgg cattttgctg ttttttgtgc
    27901 tcctggggta ctcaggatgg actgtccgga cgctctgccg gcgtctgtac agcctggtgc
    27961 agggtaaaca ggcgtcaggc cgtccctggt ggtaccggca tcgcacagaa ttaccccgtg
    28021 aatggcgcgg aacggaataa ccctcttttg ttcatcatcc cggcgtatca caacgccctc
    28081 atctttaatc cccggagaac gacatgaagt ccagatatcg aacctggctt gctgtcccgc
    28141 ctgaggagac agaagccgta aaaaatgcag ttcccccgtt aaatggccgc aaagctgtcg
    28201 catgggatcc agaaaaaaag ctctggtatg cccgggcggg taccgaactt tcactgctgg
    28261 agcgctggtt accccggcca caggaactgt cgatggatgc cggcgatccg gtcactgaat
    28321 ttgctcaggt cctggaaaac gccgggctgg ttattcaggg attgccacag atggacggag
    28381 ccatacacag agtgaccacc cgggatgaca aaaaaggggc caaaagtggc gcatacaggg
    28441 cctatcttga tggtcgtccc gccggctggt accgcgacta ccggagcgcc gatgacagtc
    28501 ccacaaactg ggtattttcc ggcggagaac aacacgatcc tctggccaga ctgcatctgc
    28561 gggcattcgc tcagcaacag cgcgatgata acgctcgtaa actgcagcaa cagtataaca
    28621 aacaggccgg ctatgcccgc tcctatatta acaggttgcc acaggcaaca gcacatgaat
    28681 acctgacgcg caagggcatc cgggccgcgc ctggcgtgcg gctgaacaat aaaaatgagc
    28741 tggttattcc cttcagcaac ggccggggag aaatacgttc gtaccagcgt attccggtca
    28801 ccggcgggaa ggatgcccgc attctgaaag actccgagaa aaccggcaac tggttcacct
    28861 tcggaacccc ggaaaatggc cgccccctgt tatttgcgga aggttacgct actgccgcct
    28921 ccctgcacga agcaaccggc cttccggttc tgatgacagt tgatgccagc aatatgattg
    28981 ccgtggcaga aaatgcccgc caaatctgga ctgacagccc ctttgtcttc tgtgccgaca
    29041 acgatcatca acgggaaatt aacaaagggg tttttagtgc aacaaaggcc gcagaagtca
    29101 cgaatggcga agtcattatt ccggccttta cggaggcgga aaaagcacag ggactgaccg
    29161 atttcaatga cctggacgcc agccggggac gggacaactt tcagaatgcc atgaatgcac
    29221 agttaaaaca tatcggtatc cccacaccca acagtgacac tgccgaccat cgtgaggctg
    29281 tcgtgatcga caacctgata ttcacaccgg taaaagatga gaagccacaa atgtcccctg
    29341 aaaacagaca gagcacagcc ccggaaacgg agctggcgac acaggatacg ccttatgaca
    29401 cccactccga cgatacagaa aaccaggcat atgaagatga ctatgccgcc tatgcatccc
    29461 tgcaatcagt aactgcggag acgttatccc cggataaacc actggagaat attgcgtctg
    29521 ccgctgatac cggcacagag aatacctctg tgcctaccgc tggcgaccag gaagacctca
    29581 atacagacgc cacaggtaca gacaccgctc acaacagcga cacagcagaa acaggaacgc
    29641 ttcctgaacc tgccgggatg cagtccgtgc agcaggcaac cactgaaccg accgcagaag
    29701 ataaaccaca cgagaacgtt gtttccgctg ccgataccag agaagaaaat gcctctgtgg
    29761 ctaccgctga taccggtgaa gaaaatgcct ctgccaccac cgctgacaac cagagcacgc
    29821 aggcagaaga acatccccgg agtaaagaca caccggaaac ctccctctcc gacgcggccg
    29881 ggacccagca caccaccaca acgcagtctg aagaacaagt gcaggtttcc atggccagcg
    29941 ctgacgactg gagcgcattt gcggaagatc tcaacgctga cactccgggg gcccccactg
    30001 ttcacaacac cgacaacgcg gagacaaaca cagtttctga accggccggg atgcggccag
    30061 ttcagcaggt gaccactgaa ctgacggcag aagataaagc acaggaaaag agcgttgcgt
    30121 ctgccgctga gaccggcgca gagcactcct ctgacatcac cgatgacaac aggaacgcac
    30181 ccgctgagga gctcaatgcc gatgccacgg ttgctgttca caacaccggc acaacagaaa
    30241 cagcctcacc ttctgaacct gcagaaactc agccagtccg gcaggccacc gctgaaccga
    30301 cggctgaaga taaactgcag gaagagcgtc ttgtttccgc cactgagacc agcacagcga
    30361 atgaagcagc ccgccctgaa acgccatcgc ttttcagccg tctgcgccgt tttctgaccg
    30421 gtgtgaaatc acgcacctca gaaccttccg ttattcagga gaaaaagccc acggaggctg
    30481 aaacagcaac agaggaggtc gcaccaccat caccaccgga aaccagcccg gaagacacat
    30541 cgtcagcgga accggacacg attatctttg caccccgccg tcccgacagt ccgcagcggc
    30601 acaacctgga cgagatcctt aaaaatctta cacacaaaga gtttcccgac aggactgcgc
    30661 tctacagcgt tgacggtgaa cccgccttcc tggacagact ctactgtctc gaaatggtgg
    30721 atggcgccag cgcggatgac aaaaaggtcc tggctgccct tgctgtggcc accaacttct
    30781 atggcggcgt tatcgagctg accggcagtg atgcctttaa gcaaaaagcc atgcagctga
    30841 ttgtcgaata cgacatcaag gttcgcatga aactcccggc ccagcgggca gcactggaaa
    30901 aaatccgcaa ggaaaaagag atggcgcatg atgccattgt cacccacaga cccacgccgg
    30961 aactcaaccg ccagtcaccg gaagagacaa caatggccca accggctccc cctgtgtcac
    31021 cgaaggcaga ggctgaacaa acagcaggca gtacggctga aacaccgcat ccggaagcgc
    31081 cggtcacggc aaaagctgaa gccccccggg ccgacagacc ggaacaaccc gccgccccgt
    31141 cgtctgcgcc agaaaaagcc cctgtcagtg cacagccgca gcaacccgac acaccggagg
    31201 aagagccgcc cggcaagctc agccccggtg aaagtgtgac tgccgtcctg acaaactacg
    31261 gagaaaaaga gtatgcgccc ggaaaagggc gctgcttttt tgtggaactc accaaccgca
    31321 gtggcaaacg ggaatactgg ggaaacggcc tggaagaact ggtcaaaaac catcagaagg
    31381 gtgaccccgt cacactgaca ctgcaggcga gagaaacatt ccggctgtcc ggtgaaacgc
    31441 gggatcgtat ccgtaatatc tgggccataa ccccggtcag taacggcatt accgttaata
    31501 atgaccaacc tcatgcgggt cagcataccc tggagtttcc gatggcaaca ttcagccagg
    31561 tgatgttaca gatgcaacaa cactggccac agataatgca ggaagttcgc tcccccaaaa
    31621 atcctcccaa taatctgtac attggtgaag acaggcaacc tgcatcagca cctcaggatc
    31681 aatcaatgcg actccccctc gctggtaatg cccccaatga actgatacca gccatcgcct
    31741 ctgccgataa ggacaatcgt cagttgaatc ttctgctggt ccacagcgca gatgatcatc
    31801 tgcagggcgt ggtccgcctg aacggcaccc tttacccggc actggccacc ccgtcagctg
    31861 acaaccgtca gctggttatc aatgccctga cggataacgg tctgcagttt gccggttacg
    31921 gtgaagctgt taaccatgat gagaataatc atcagcgtcc gtccccacaa ataatgcagt
    31981 ttcacctgaa gcaacaggac tccccgttgt ttgccgccat ccacaaaccg gaagagcaac
    32041 cggacaagct tttccgctct ctgggttttg agcagacctg gaaagagtgg tctgacagcc
    32101 aaaaggcgga ggacaggcag gaaaaaactc tgcaacaggc acaatcccac agccccggcc
    32161 gatgagccgg taaggacgac catgaacatg aaaaaaacgc tgctggtcat actgaccggc
    32221 atcaccctgg catcaccggt ggcaggtgcc gccacctgcc gggcgggggc cgctgccgaa
    32281 gccggcagca gagccgcctg ggagcgggcg cgcacagcca gccaggcatg gtcacagcgg
    32341 gagaatgacg tcgcctcctc cctgcagtcc tgcctgtccc gcatcaagga catcagtatc
    32401 actcttccgc agttccccag cctggacaag ctcctccgcg acctggaaaa ccaggtctgt
    32461 gatgccgtcg tggatcgggt gaatgaccat cttccgggga atatcgaccc ctggaaagac
    32521 tacaacctct gaacaggagc accatgagcg aagcacaacc cacctcccct gtctcaccgg
    32581 aagcggataa cagcggagat atttataacc ccgtgctcag aaccatcgca cagtcagaac
    32641 aacgggccgc gctggtaccc ggtctggtca gggcgttgct gtacagcggc gcagcactct
    32701 gcatcagcct ggtggctaat ggcatacagt actggcatgc caccggcgtg acgcgcgaat
    32761 actttgccac tgacaatggc cgactggtcc gtctggcacc aaccagccag ccggcctgga
    32821 gtcagagcga cgtcatgaac tttggcagca tgacgctgag cgaagccttc aatctcgatt
    32881 ttgtccacta ccgaaaacaa atcacgacac aggccccccg cttttccgaa gccggctttg
    32941 ccggctatgt gaatgccctt caggcctcga acattctcga cactattaaa aaggagaaat
    33001 ttaacctcac aaccactatc ggggcaggta ccctggtcaa ccagggacag atggagaacg
    33061 gcgtctggtt ctggaaattc cagtatccgg tcaggatgcg cctggtcggt cagaccagca
    33121 cccgccccga acaggccttc acctttgaaa tcactgtccg gcgcgttgac ccccgcatca
    33181 aacccggcgg catggaaatc agccagatgg tttcccgtaa cgccgcagca ggaatctgag
    33241 actcatatca tgcacataca tcatcttatt ggcgcactgc ttattgtgtg cggcactgtc
    33301 acagctgcag ataacccggg ctggaaaaat gcacgaagcc cgcagaccgg cggaaagacc
    33361 gcacaggcaa ccgggaacac cccggcaggc ggtcccccgc cagccgcaca actcccggga
    33421 aacctgccgc ctcccggcgc tgctcttgta caggaaggcg catcgctgga ttctcccctg
    33481 aatgccagtg aaattcgtga actccgtggg ataatggccg ataacgaacg ggccatcagt
    33541 gccccgctca ccagtgtggt gccccgtatc agttccctca cggtgaatct gtctccgggg
    33601 gccagcctgc cccttgtccg taccgctctg cataacgtca gcgtggtgac cttcacggac
    33661 gtcaacggca gtccgtggcc acagtcggat ccgccctata acgctgcgcc cggtcacttt
    33721 gacgtgcagt tcaacggcaa tatggtgtcc gtcacccccc tgcgcgcctg ggctgccggg
    33781 aatgtgacgg tctatctcaa aggattgtct gtaccggtca tcctgaatat caccagtggt
    33841 gaaacggaca ccactaccct gagtcaggaa atcgacagtc gtctggatct gcgcattccc
    33901 cggcggggac ctgccagccc ggtggtgtcc ataccgtcag aaaaaattgc cctgcatgac
    33961 gccactctgc aggcctttct ggacggtgtg ccaccggatg atcccgccat ccgccgcctg
    34021 aaattctccg gtaacgtccc ggacaccacc atctggcaac acggcgatga tcttctcgtg
    34081 cgctcacggg ccatgctgcg ggatgaattt gaacagacgc tggcatcggc tgatggtacg
    34141 cggctgtgga aactgccggt gaccccctta ctcaccttct ccgtgaatgg tcagtccgtt
    34201 cacgtcaccc cggaactgga gtaaatcatg agcgcagaac aagaacagga cacagccgca
    34261 aaatccggca aaaagctgtc agccctgctg gttatggggg gcgtgattat catcgggggc
    34321 ggatacctgc tgctcacctg gctgtccccc gaaaacgctg catcgctctc gtcagtcaat
    34381 atcaacagca caacgtccgg cggcggacgg aacgtgatgg agacacagca ctaccgggat
    34441 ttactgcggg ctgacaatga acgtggtgct gcagaagcgt tacgtaataa cgagacgttt
    34501 atcgccagtc tgccccgggg actggatacc gataccggaa acgatcagaa aagggataat
    34561 ccccccgatg atcagtcagg cacttccggg caaaacgcat ccccccctca ggaagatcag
    34621 actgcccggg aaaaacggcg tgaggcatta cagaaactgc ttgcccgtat caatacacag
    34681 catcaggatg cccggccacc ggtagtggca gcagccatgt ggagcaccac cggcactccc
    34741 gggacagccc cacatgcgtc accgtccggt accgttacat cacagcatcc ccgtctgcag
    34801 gagacgagtc tggcaacacc ggctacacgt caggggatcc agctgatacc ggcactgacc
    34861 cgcgtaccgg cttacatcga caccgaagtg gactctgaca atgcggtctc ccgtgtggtg
    34921 gcctccgtac ctgccggccc atgggccggg gccgtcctct acagccagga cgccagactc
    34981 gcaggtcagg gaatggatat ccactttgac cggatgatgt ggcggggcat gtttctgaag
    35041 gtgaatgcat atgccctgaa tgaacgaaca ggaatgtcaa ccgtggccag cgacgtgaac
    35101 caccgctggt ttaagcacat cgttctgccg tccgtacttg gcggggtggg caatgtcgga
    35161 agtctttaca aagacgccaa cacacagatc cttcagggaa actacggcag tattaccggc
    35221 agggtgggaa tgcctgatgg agaggcgctg gccggcgtca ttgcaggtgg catggccgaa
    35281 cggggcagcc agctgctgac ccgccaggca gagagcgaac cttaccgtca ggtgacagta
    35341 tcacggggcg aggtcgtctc cattctgttt gttgagccgg tcatgagcaa cgatatcagt
    35401 acgccgtcct ccggcactgc cccttctgag tccccctcct ccggacgtcc gctcacccag
    35461 gcggaggcag agcaccaggc ctctgacagg atccgggcgg cggtcctccg taaacagaat
    35521 gaaatgcagc aacgctacgg catacaacag gaaagttatc catgaaaaac gaacatgaag
    35581 atcccggcag ctttggcgaa acgacggcac cggcatccgg caaatccttc ctgaaacgcc
    35641 agtatatggg atacagcctg ccggtctggg ccggctgtgc cctcatcatc ctggtcgcca
    35701 tctggtacaa gtttctgcgg acaccctcag agccccctgc agatgcaggc tttgctgtca
    35761 caccgcccgc tgctgagagc tgggcagagc ccatgaccat gacggagtcc gggatcggaa
    35821 acaacatgcc cgcaccgccg gttaccccac aggcagacat gcagcaactt gcccgtgatc
    35881 tgaaagcaga gctggacaga cgggatgagg aaatccggcg caccctgacc atgctgaagg
    35941 acagtatcac caccctcagt aacgccattc agaaggatga ggcctgggca caggaaaccc
    36001 gtcagttgct tgaggagacc cgtaagcagg tgcataccct gcagatgcag ggagcactgc
    36061 cagccccgac cgggaataac agacagcctg cgaaaaccct gccttcacct ctgcagggca
    36121 tgaaaatcat gtcggtggag aaaggcatgg cctggatccg ctggcagggc agtgcctggg
    36181 cagtacggga aggcgacagt ctgggaaaca tcaccatcac ccggattgac ccgtccagcc
    36241 gggcagtcat cacctctgcc ggtacattac gatgatgagc attgatgccc tgacagccat
    36301 agagaacttt gccagcggta ttttcagtgc aggcatggag tttctcttta cctggggaga
    36361 gctgctgggg attatcggcc ttatcggtca tctgatgcgc gcccgggcag aaggacgtca
    36421 cagtatggga ccgggaaaat ttattgccgg tatcttcatc tgcggcatgc tggtcagtct
    36481 gccctcaatg atcaatgccg gtggtaccca gatgggcttc agggcagatt ctttcgcccc
    36541 catcgcatac gtgcagccgg gctcctttgg tgccgctgcc ggtgccgtca atgccattct
    36601 ctcactggca aaactggccg gcgtcggttt tgtcatgaac ggcatatcca tctggcgcaa
    36661 atcactgctg gacgggcaca ccgccctgag cgccagtgaa tccatcagta aagggaacgt
    36721 caagtttgtg gccggcgttc tgctggtttt caacgaccgg gttatcgatg ccactctggc
    36781 aagcctcggt atcgccttct gacccctgta ttttttcagt aaatcaatct gttaataaag
    36841 gagacactat gtcacgactt cgtgccatgg cggcgcgtct gcgcgctgca tctgtttatg
    36901 ttcagttatg cctgatggcc tgccgtgacc ggctggtacg cctgttactg ctgcttcccg
    36961 tgatgctgct gccccacacc gcaatggcaa ataccctctt cgatatgatt aaaaccgttg
    37021 gcgatggtgc cagaggctcc agcagttcgt ttgtggctat cggtcaggtt attggcctgg
    37081 tgctctttat cggcggactg cttggcctca aacaaatcgg taaaaaaccc ggtgcagggc
    37141 tggcctgcct gctgtcaatt ctggtcggcg tctgccttgc cgtcgtaccg gaactgatgg
    37201 gcagcggaca gaaaacgctc ggtctttctc cggtttcaat cagttaatcc gcaaaggcac
    37261 cgggaaaccg gtgccgtctt cacacaatga ggaggaccag accatgtcct gtgagctacc
    37321 ccctttcacc ctgctcagaa cagagcacgg caacgtgatt gcccgcgaca accgggataa
    37381 tcaggagttt gtgtttgcag agtgttactc caccgctata gcagcactga ttctggctgc
    37441 cgtcaccctg tatacagaga gaaaagcgta tgaaaaagac cccgccactc atcgccccgg
    37501 tccgggaacg atggcttttc ccgctcccgc aaccggacac ggtattcagc ggtgaagccc
    37561 tgttaccacc gccagtgctc tcaacagagg gacgctgccc ctgctgtcgc aggaccgtca
    37621 cccaccgctt cattctggaa gattcatggc cattacagca gatggctgac acctgccggg
    37681 atacgatcgt cctgctggaa aaaaacctca cccgggtgat gcgcctgaaa aaacgtccgg
    37741 taccggaaaa cgctgacgaa aaaaagaaac acacccgaac cctgcaggat gcggagcggt
    37801 ccctggcaca ggcccgcctg tcggccagac gactggccct gcgtcatgtg gaaaagtcgc
    37861 aaatcgtcac aacggatgca ctcagcgaaa atgaaagtga cctgctgcag ccggaaggcc
    37921 cgccctttca tctctgtgcc ttctgccacg cctggcactg tctgaacggc tacgctgccg
    37981 cacagggagt gatggtctgg ctccccgatt tgcatccggc ctcggtggtt gccctcaatg
    38041 cgcgggcact gcaggagatt ttcagcgata accgtcagcg ggtcaggcag ggacgggcag
    38101 tcctgaatgc cctggtacag aaccgtctgg ccgttgagga gaaattccgc acctggcgtc
    38161 cggcggattt tgcagatgcc ctgcgccgct ggccgccggc acagcgtaaa acgttgaggg
    38221 agaaaatgga cggggtggcg ctcattctgc tgccggattc gtttcctgac aaaaagtacg
    38281 tcatgtgaca cacaccggtg ccggtgacgt tttgccggat atcagttctc cgcagatgca
    38341 ttccggcact ttattatccg gtcgctgact gacgatattc cgcctgacgg agatgaaaaa
    38401 gagattgccc ggttcattcg ccgccagcag tttatttatc cgggtgtaat cacacagaaa
    38461 tacgcccctg cccctcaaca tattatttat cttctctgct gttttttcat gccacatccc
    38521 ctgctgtggc atcaaaaaat ataagacctt gatgattcat ccttaccgga gtgccccatg
    38581 ccatgaatat caaccgcatt atttttgcca ttgaagactg tatcagtacc ctgtcacggt
    38641 attccgtttc cgcattcttc cccgattact gtgatctgca caccgtcatc ggtctggacc
    38701 agcaggaccg cgaacgccgc ccctggctga aagcccccta cattgccacc acaaaacagg
    38761 ggtattacct ttccgtcttt gaaatcagcg gtgccctgaa agaaatggat gaaaacccgg
    38821 accagacggc ccccgggaca ctggagtcac tgattgcagc actggctgag cgaatgaaca
    38881 cggcctggaa aaattccggc cacaaaatca gttttgtttt cgaacgcgat ccggagcgtg
    38941 gaagggatga aatcgaagcc atgctcttac cccagcgaaa atccatcgca cgcacgggga
    39001 tcctgcttca ggacgtcctg gatgaaaaag tcaccacact caccccctgg cttgtccggg
    39061 agcgctgctg gctgaccgtc tggagctctg aagaactgct gagccggacc gatcgcaaga
    39121 accatcagac ccgtgtcagg aaactcgctg aacacgctcc gccagcccgt tttgcgcagg
    39181 atccctggcg ctggacactt tccgccctca aaatacggca tgacgcctta ctggacacgc
    39241 tggagcaggc tctgacacat gattctgatg gcctgctgat tcgcctgatg gatatccatg
    39301 aagtgggacg ggaaatccgc cgccaactgg agcgcaacag tacaccggca gtatggcagc
    39361 ctcatctgcc cgaagatgcc cgtcctgccg gctggcgaca gggcggggat acctctgtat
    39421 tccacgcacc gtcgctgaat ctgcagttat tcaccacaca gcctgaaaca cacggcagcc
    39481 tgattcaggc cggtgagctc tggcacggta tggtcgccat taccctgccc cctcagaatc
    39541 tgcggacatt caaccagctg gtcagggatg tgccccgtgc aatcccctgg cgtatccgga
    39601 tggatctgat gcccgacggc atgaaggcgc tgggggtcaa gaaaaccctg ctgacctaca
    39661 gcagctttat tcctcctctc cggcccatgt atgactcggt aatgatgctg aatgacatca
    39721 acaagcatga tccggtctgt atcatgacca ttgttgccac cacatgggga aacagccggg
    39781 aagtctgtac ccgtaaccag gctctcctga aatctgcgct ggaaggctgg ggggtatgcg
    39841 gcaccaccac aaccttcggt gatccgagac gtgcctgggt gaacacggtt ctggcagcct
    39901 cccacagctc cggcccgatc cccctttacc cgcccctttc acacgccctt tccctgtttc
    39961 ccctgaacag ggcaggctct gtctggcggg gacagggaaa tctgatgatg cacaccgaag
    40021 acggctctgc atgggaggtg gcgctggcgt catcacaaca gaacaagcac acggagctga
    40081 cacccggcgc acccggtctg ggcaaatcgg tactcatcaa cgcactgtcg gagatccaga
    40141 tagcctcagc ccagaagaat ctccctttca ttgcctatat cgacaaggga ttcagtgcac
    40201 agggactggt acagcttatc cgtgacagcc tgcctgagca gagaaaggac gaagccgtgg
    40261 ggatcattct gagtaatgac ccggaccaca cccgcaacct gtttgatgtg atgtacgggg
    40321 ccagaaaacc cgtcacaccg gaaaagaact tcatggtctc cgtcctctgt gcattgtgtg
    40381 tggataccgg caccggtcag ccctgtaatc ccggcgacac ccggcaaatc atcagcagcc
    40441 tggtggacct ggccttccgg gaatatggcg agaataaccc cagactttac cgcgcaggta
    40501 cggaacccct ggtggatctc gccctggaag agagcggcat cgctgaacaa catgatgccg
    40561 gatggtggaa tgccgccacc tggtttgaaa tcagggacat gctgcatatt gcagggaata
    40621 tccccgccgc ccagcgggca cactaccagg ccatgcccct gctggctgaa atgtccgccc
    40681 tgctgggaca gccgtccatc cgggatgtgt tcggcacggt tcagcgtgat aactcggaag
    40741 agcggttgct ggactacatc cgccgtgccc tcgatcaggg acacagtgac tacccgatga
    40801 tgtccggctg tacccggttt atgctcagcc ccgacacccg cgtggtcgcc gttgatctga
    40861 acaatgtggc cggggacaag acacctgccg gacgtctgcg caccggtatc atgtatctgc
    40921 tggccgggca gatcgccggc ggggattttg tcctgccgca gtatcaggag gaaatacgga
    40981 aaaatctcga cccacgctac catgaggtga ttttccgccg cattgaacag cttgatcagg
    41041 aggtcaagac caaagtctat gacgaactgc acaatgcaaa agggatcaac ttcatctggg
    41101 aagccctgga cacccaggag ctggagcagc gtaagttcgg gatacgcacg gtgctgagca
    41161 cccagctgct gcagcattat cctccggccg tgctcaaatc cgccaacacc ctgtggctgc
    41221 tgcgttaccg ccctgacgaa attccgttcc tgcgggataa ctttggcgtc ccggaagtca
    41281 cactgcggcg cttcctgaaa atgccggaag gggccgcacc ggacggcagc ggtgtcccgg
    41341 tactggctgt gttccgtgta aaaaacggca cgctggcccg gatcctgaaa ttcaccctcg
    41401 gtccactgga gctgtgggca ctgaactcct ctcctaaaga cagcgccctg cgacgtgcgc
    41461 tgacgcagga ggtggggtca ctgcgggcac gccagatcct ggccgaacac tttccccggg
    41521 gttcagccac ctcactgata gaacaccgcg cccgcacaca tgacagtgaa aacgtcatcc
    41581 atgagctggc agcagaactc atccgtaagc agggatataa cctctgatgt acagcattca
    41641 tatcaccccc cgctgccggc gtcctgcccg gggacaggtc ttcctgaaaa cactgcgcgt
    41701 ggacggtgac agtttcagct tcaccctgcc gtccgcctgc ttcacactgc gaccgtcccc
    41761 caatccccgg gtcatttttg ccagtgacag acgggctgac ggggagatga gtggtattcg
    41821 cctggccctg cccggagaga caggcacgct gataacaacg gccacctggc aggttaacgt
    41881 gccggagcga ccgccttttg atatacagca tgtcattcac tggcttctgc ccgcaccact
    41941 cactgcgctg atgtctgaca acacctggct gtggccggat gtccccggca caccggcact
    42001 cacagccgga gactggccgg tcatggaaag cgacaccccg acactgaaac gcgtgaagcg
    42061 tcgtaccttc acgctcatcg ataaccgtaa ccgtcaggga tggatcacgc gccgcttcca
    42121 gtgtttcccg ctggctccgc tgccggaatc actcagtcat cgcgtaccag acatcctgta
    42181 tcccccgaaa acggagaaaa aagaatgcaa agacaatctg caaccacact gcttgcactc
    42241 cttgtaggct gtgcggttat ccctgccacc catgcctact ctgtgaatgt tgtgtccagt
    42301 cagccggtca cccgacagat catgccggag ctggccacca tacagggcat catggaggaa
    42361 atggtccgta tgcagacggc caccggcaca gccatcaccc agaactcaga aaaactggcc
    42421 accgtcattg cccaggacgg gcaggccacc cgtcagcaga tgatattcag taatgaaaca
    42481 caccgcctcg aggaggccag gaaaagcttc agcgtgccgg actccatctg cagtgaatcc
    42541 gcgtccggca ttgcagcaga aagccgccgg gcagcagcgt ctgcagcggc acgtctgagt
    42601 caggggggag gggtcagcag taaacccatc cgggaacgtc tctcccgggc tgcggactcc
    42661 ccggtccgcg aggcgtatga tagtgccggc atccacgccg gttactgcac ggaagcggag
    42721 tatgcccgct ttggcggaac tgatgtctgc cctgccgtgg gcgatctgcc gggcggagac
    42781 agccaggtac gatcgctcta tcagggcgca ggcacagcag ataccccggc cgctctcacc
    42841 tgggatcaga aacagattga tgccgcgacg gcttatatga aaaacaccgc cagaccgtca
    42901 gcgggtcgtg ccccggggaa aggagaagtg ggcacacaga ccgggcgcac gtatgtgggt
    42961 ctgcagaatg aatacaacgg catcatcgat gccgcctctc atccgcagct gtcactgatt
    43021 gctgacagta cccctaatga agccacccgt ggggcactga ctgaagccct gcagtcaccg
    43081 tcagctgcgg catattttga ccggaccgca tcatcagaag cccgcacccg tggccacatg
    43141 agccagcggg agtttgaggc ctttgaagcc ggcagacggt atgccaacac cgactggcag
    43201 caggatctgc agggcatgga aggcgataac cttctccgtg agctgctgcg caccaccgcc
    43261 ctgctgaact ggcagatgaa cgatctgaaa gagcagattc gtcagggtaa cgttatcgcc
    43321 gggcagcaac ttgcgctggc cgcacggcag tattacggac agcgccttgg cgaactgtca
    43381 caggccatgg gccaggggag cgtaagatga aagacgacaa cagcatcgac gaaaaaaaac
    43441 cggccccccg tctccgccgc atcggtgata tagggataaa tctgttcgca ccactggccg
    43501 aaacccgctg gatgctgcgc agctgccgcc atatgctgga ccagcataaa acgcgcctga
    43561 agcgcctcat gcccgaccgg caaccggaga gtccggtctg tatgacctgg gaggaagcgg
    43621 tcagggccag cggactgacg gtcgacgaac tggaccggcg tctgcgacgt cgcaggatgt
    43681 tctggcggac aggattctgg ctgatgatga taccggtact tctgctgtgt ggtatggccc
    43741 ttgcggccac cgcactgccg gtcatcaccc tgatacgtat cctggtctgc ctgttactga
    43801 tgagtaccat tgcagcactg tgtgcctcaa aagccctgat tgcgtcatac cgcctctggc
    43861 agctgaatga acgtcgggtc agcgaggacg aacagggcac attccccgat tacctgcagg
    43921 cccggcacgg ctggaaacag gccacatggc tggccattac ccaccggcag tactgactgt
    43981 ccggtctgca atttcttttc ctcattccgg gggctgatgc ccgggcatcc ccttcggttc
    44041 cggagttacc gtgaaaatca tacccagagc actgagtgca ggtctggcgc tggctgccgt
    44101 acctgcacag gctttcgtca cctatcagga cattgtgagt gccgccacca atccggatga
    44161 cctgtcgcgc caggcactgg tcaccatttt tggtgatgtg gtcacaaatc ccctgacagc
    44221 cagcagcaac accatcattg gcaacctgtt catggtgttt aacgcaatta tcgccacgat
    44281 ggggatagtc tggtttctgt ttattggtat ccgtcatgct gtccgcacgg gacaccaggg
    44341 cagtgtcttt aacaacggac gggacgtcgt cggcatactc agtgtcgtga gtggattttt
    44401 gatgattgtg cccaccgctt ccggctggag tctggcgcaa ctggtcatgc tgtggggcgc
    44461 atccatcatg ggcatcggtt ccgccaatat catggttcag acagcggcag aaaacattgc
    44521 tgaaggctat tcaatgaccg tacagccagt tcatgtctcc acccgcacgg cagcacgggg
    44581 aattctggag atggaactgt gcaaacacgc catgaatacg ggcctcaatg actttaaccg
    44641 taccgcacgt tcatccacgc cgcccatgag cgaatcctca cggacagaga atggcagata
    44701 taccgtcacc gtctcaaatg gcagcgccac ctgcgggagt gtgagtctct ccgtgaaagg
    44761 aaacggcagt aacaagcagt ccgcgctggc ccattttttc aatcccttca ttgaaacgga
    44821 atatcagggg gttctgacgg cacagcgctc cgccatggat accatgctcc gtgacctgga
    44881 cagcgcggcg caggaatttg tgtcggcata ccggacccgg cgcgacacgg gtaacggcaa
    44941 cctgccggat attgaaagcc gtattcagaa ggcagccaac cggtatgaag aaacggtgca
    45001 gaaggcgttg ccaacagata acgcagaaac tgagcgcagg gagcagctga aatcctacct
    45061 gaccacctac ggctgggttg ccctgggcgc ctggtaccag acctttgcca ccgccaacca
    45121 gcgactggct gaactggccg accgtgctcc tacagtgagt ggcgtctcgt cactgggcga
    45181 agccggggat acggaattcc tgaaggaagt caagggggct taccaggcac agctacagaa
    45241 ctcagcgtat actcctgcac tgggaacaac cacctctgcc agtgaatacc acgcatctaa
    45301 tactcaaaat cctcaatcag ccctgttaga gttcataggg ccagggttaa ctcgattttc
    45361 aaataaagta gcaaccatac attcaggaac aggaacaacg agtcagcagg caaatcctct
    45421 gctgaagatg aaggacattg gtgattacac catggtcaca accgaaacca tctgggcggg
    45481 ttataccacc gtcagggtgc tttctactgc cggcagcaac tccgtttttg gccgactggt
    45541 caatgccgtc agcggcttat ctgatgcagc aaaggctttt ctggaagcct ccgcaccacc
    45601 actctacttc ctgctgtttt tgctgttctg tgccggtttt tcgttatcta tttacctgcc
    45661 gtttatcccg tttatcttct ggatgaccgg catcggcaac tggatagtca gcgtactgat
    45721 tggatgtgct gctggccccc tgtgggccgc cacccatctg ggaacaagtc aggacagggg
    45781 aagccgggcc gcttacggtt acatctacct gatagacagc atgatcagac cgctgctgat
    45841 ggtattcggg ttcttttttg catcggtcgc catcgtggcc ggcggcacaa tactgaatgc
    45901 gctgtttgct gctgcgctga tgaatgtcca gataaactct gtgacgggcg tgttcagcat
    45961 ggcgggattt ttgatgattt atgcccgagc ctgtaccacg ctggttgcgg ccatttttgc
    46021 acttcaggct tatctgcctg accatgtaat taattttctg ggcgggcgtg acggtgttaa
    46081 tatgctggga aatatggttt cttcagtcaa agatattatt gccggaggta ataccaatat
    46141 gcgcagaaca cctggcgttc gggaagatag actgaaaaat attaatagag atgataaaga
    46201 cggaataaaa caataaaatg aaaacataga aaaaccaata aaacagcatt gccatattca
    46261 cctgaagagt ttaaaatata ttcaggtgaa tatggaggtt tattatgaac aaaacaaaaa
    46321 gcagatatcc ttcctggtgg gcatattacc acttggcccg caacgcttat ttcttattag
    46381 gaataccgtg tttactttta tactcagccg cttatacatc agatttatta gatgataatc
    46441 taagacattc aataagtctt tttattctgg catatggatg ttattttctt cttttcattc
    46501 ctccgctctg gttatatctg agaaccagag cgaagaaaaa gaaagtccag caggttgttc
    46561 agcagataaa agaaagcagt ccctttgcgc cgaccagcga ttacgagcaa ctgtcattct
    46621 caaagagttg ttatttcggg atcgacatta aaaacggcac catgctgtat gtgcgcattt
    46681 atccgaacaa tgtgatggat gtgattggtc tggatattca caactttacc cgcacggttg
    46741 ctgaggacgg caagctggaa atccacacca cctatgtgag tctgccgatg attcctcttg
    46801 agatctccgg catctcagca aggacgctgg ccaacaccat gcacaccatg gctgcacgtg
    46861 gatatgagta caaggaacgt ttcccacaga tgatccggta tcgggttaaa gagtgggaaa
    46921 aagttgccgg tgttcctgtt gcagaagtat tttgaggtag ctatcaactg ttagacgata
    46981 atccgtcagc cggttttctc tgaaccggct ttttcttttc aggcagaatg cctgtctgcc
    47041 gtctatctca ggacgccgca tggagtctct atgttttccc cacaaattct tagcactgtc
    47101 ttgcccactg tagacaaaat caatacaatt ctgcatatca gttatctata aaagataaca
    47161 atatcattat aaataattat catatccaca cactcaaggc agtacctaga aaacataaag
    47221 ataaaatcat gtctgaatcc cgcagcaatt ttccagcacg acatggtact ccatggaata
    47281 ctgaagagtt acagcgtctt ctcgtatcca tagcggaggg cgtctcgcta aaaaaactta
    47341 ctcaaacaca tcaacgatcg tccgggggaa tacgtggagc aatcaaacgc ctgcttcctt
    47401 ctgagtttct tgagttcaac gatataaaca cggtgaatga tctcagcaat tacttcaaca
    47461 agattcctcg ccaagaacac aatagtatca tcaataattt aatatcattg agcactgcaa
    47521 aaacaattgc tcatgataaa gttgaaatac aaaagaatag ttttaaacaa aataccccac
    47581 tccccctctg ccaacccaat acagatttta aagattatat agccgatgaa catgatgtca
    47641 ttatgctaat tcattctgct atcgaaagtt tatctaataa acgacatcaa tatattcttc
    47701 taatgcgact cggcacagat gattcacctc attctctggc agatattgcc gtcaaattag
    47761 gaatttcccg tgaaagggta agacaacttc aggaacgagc ccttggctta ctagctgcta
    47821 aatctcgcta tgaagggaca cctggagctt gccttaaagc ccttattaac tcaataggta
    47881 attcaccata tgatatagca gtatggattt atcgtattgt tcatcgggat ttcgttacat
    47941 cctctgagtt agccgccaaa ttcatcattt ttgccgcagg cattcctaaa gcaagacgag
    48001 atgaaataaa aaattcgctc tcattaatat cacaattaca aaagcagaag ttacgtgaac
    48061 gaagagcaga attagcacac gctcacgctc aggaaagagc ccgaatcaag aaagaaagag
    48121 ttgagttcat attcagtaaa tggctagaca atacacattg gccaaaatat atcgcacctc
    48181 ctccccccgt agaacagcta tgttcacaga gagcggttaa tgatactgac atatctggat
    48241 tcttttactc tcaaaaatta ggtcgtacag tccagtacga atcagggctt gagtttaaaa
    48301 taatgaatct attagaatgt tgcgatcagg ttcttttcta tcaagaacaa ccattttcta
    48361 ttccatattg ttttaaaaac aaatcaaaaa aatactatcc agatttactc attgcgacag
    48421 ttgatggtcg gtgcctactt atggaagtga agccaacaga ccgaatgacc ttgagtctca
    48481 cgagaataaa aagtaatgca ggtcgcaaat gggcacatac tcgaggatgg ggatggttgg
    48541 tcattagtga taggttctcg ttacgtcagc tggaagaaca tgccatctca acaaacacat
    48601 ggaaacttat agaggctgaa ctgaaaacat caaggatttt agcatggcgt gaaatgatgg
    48661 agttacgtac acgatacgag attcatcgtt tagatcttat tgcatatatc attcagtctg
    48721 gtgctgaagt ggataacttt tatcgaatca ccccgaaaat atctaataat acatctaacc
    48781 agagacaacc aaatgagagc gactgtcata attgatgacg cacaggacgg aaaaaataat
    48841 agctgccgga cagcagcaac cagcagtcgt ccggtaaaaa agacaaacaa ggcgcattga
    48901 ggtcagcctt cgcaacaatc cggcggcaga tgtcctgccg ccgtgttgcc cgttaacgtt
    48961 taacttcgta ggctaacgtt gccacaagca ccgtatttcc ctgctcgata cgaaatccgc
    49021 aaagcgaatc cctcaccatc cagacaaaac acagtatcgt cacacagacg acgattaaca
    49081 tcattaaaaa cgttcgttgt ggcatcgcag cccctccatt gcctttcggc cgtcatcggg
    49141 ctactataat gttgttgagg cattacagtg ggccctgggt tgattgataa aaaaatcgcc
    49201 tggggctttc tgctacatgc cgtccacaaa acgctttcgg catacagcct caagcgcccg
    49261 ccgctattct aatcgttttc ctgtccgcca caatcctttt catgccacca cgtattttgt
    49321 gtcagggaca gaaataaagc cggaaagtga ctacgtgtgc acacatctgt tttccgggct
    49381 gcaggcatcg tgttattttc tgaaatgcaa aataataaaa aacaaggaca catcatgaag
    49441 gatgaaatac acagtaccag cattaacccg gatgttgttg agcagtttct ggcccttatg
    49501 aacacacatt gtaaatcctc agaagaggca ctcgccatgc tgagtacgct ttgttacaac
    49561 ctgatgatga catgcggtat ccattccatg gacagccaga tgctcaatgg gcatgggata
    49621 tccatgcaac ttcaggggat tgatattgcc gaaaggccag gcaagaatat tcactaacct
    49681 gaacagggtc tgaaaacgct gatttttcag gtctctgcag gctaaaactg atgatgtaat
    49741 gacaacaaaa tttctgcgtt tgtaccccac cgatgaaaaa gacaaaaact cacaccgggc
    49801 ttctcatcat taaagataaa acccgacgtg tcagcctcta cgaaacgcca acggcatggt
    49861 gtatcagggg gcaggagtgc tacagcaaaa gcactggacg ccgctgtggc agccacgaca
    49921 gtctgagcag actgcgcctg gacagcatta agcctgttga atgagtggtg gcaccatgaa
    49981 agataacaac acaaccgcca ccccagaagc ctggaaaatg cgttttctgg ccaacgggac
    50041 atccggcaca tggttttaca ctgacgacat tgcagtcact gacgcgtggc ttcgcgcgca
    50101 cccggatgat gtggaggtta tccctctctg ccggatacag ccccgccagc attcccatga
    50161 acaacatatt gaagaactcc gcgcagccct ggcccggatg ctccatatgc atcatatgat
    50221 gctgaaaacc acacattacc gggaaaatat caccgatgcc cgttgtatcc gtgaaatgca
    50281 tgaagcgcca ttacaggcgc tcaatattct gtccagatac ccgcataaaa aggacgaggt
    50341 gtgacgcatg tggctactga ttttctggct ttcatcgccc tttaatgtct cttccggtga
    50401 tgcccgtcag cccacactgg cggtgcacag ccaggccttt cataccgaac gggcctgccg
    50461 cgatgcattg cgcgccatta aaaaggagaa tgttggcgaa ttttctctgc ggggcgtttg
    50521 tgtttctgac ggaaatacga aggaggaggc tgagagctca tgacggatat tactgacaga
    50581 catacacttc gccgtatgtc ctggtcggaa ctcatcacgg caggacagga agcagaattt
    50641 cagcgtgatt acgagcgtgc cagaatagtg tggtcattcg ccttacatgt tgcaacaacc
    50701 acgattaaca aaaatctggc catagctcat attcgacgct gcgatacttt gttgcataaa
    50761 tcaaaggctg tacccggaaa caccccgtgt ggttccggtc agcgagtctg agacctcaac
    50821 atcccgggtg atgattgtca ttccggactg acgcagccgc tgcacatctg cggcaatacg
    50881 ttgaatacca agccagaaca gaccatccag cgcggtgaca attaacccgc tttccagcgc
    50941 cagtttcagc cgtgctgtcg gggctttaac ctgcccggct atatcagtga aacaacgcgt
    51001 cgtcatgcca tagctgtcca cccgccgtat tcgcgtcgca agactgtaac tgaagctatc
    51061 cggcacgccg gataagtcat tctgcagact gtatacacat ccccgctgcc gctcattgaa
    51121 tacgactttt ttctggctga gctgccattc ccgccgcacg ctgtcaatca tctgcggtgt
    51181 ccgggttaac agcagacgaa acggcagttc aaaactggtc acataatcaa cattaagtaa
    51241 ggcaatccgc agccgctccc tggctcctgc tccggtatca cggcattcac gcatgacatc
    51301 ttcccactgg cgcatcagtc gcggtgactc attcctggcc ctgaactgat atttctcaat
    51361 ctgataatca atatgttcct gcataccgtc accggatatc tgttgccgta tcatcacgga
    51421 tatcagcgta ctggaattca tgccctgcgt gaagtatcac actgccaaaa aaacacacct
    51481 gaacgttatt atttcgcaat attaattgca cctgttttca taaataaaaa ggagacgatg
    51541 aaatgataaa atatatcttt tcactcacca ttattttttc cgcttctctc tgtgccgcac
    51601 agattcaggg gcacgtcatc cgcgttcttg atggtgatac gctggaaata ttacaggaca
    51661 aaaagcccgt cagggtccgt ctggctaata ttgatgcacc ggaaaagaaa cagccctttg
    51721 gacgctggtc agccactcag ctgaaggacc tggtggccgg aaagcccgta accgtgcaat
    51781 acgcgcagac agacaggtat ggccgtattc tcggccgggt ctataccgct gacggcacag
    51841 aagcaaatcg ctggcaggtc cgtaacggcg ctgcctgggt gtacccggac tacaataatg
    51901 actatactct cccgccgctc cagcgtgatg cccgcgccct gaagcgggga ttatgggcag
    51961 ataaaaatcc ggtaccacca tggacctgga gaaaacagaa taagaaataa ttatttgctg
    52021 atatgaagaa aaaaaccatt tcaaagaaaa taaatggcaa aaatacagca tggaaaaaaa
    52081 attgccattt ttaccgccgc gttattttta atatttgctg tctgctggtc gtaataagca
    52141 gaataacaga aaggtgtaaa aatgaacctg gttgatgcat ttgtcactaa agtgatatcc
    52201 ggaccgtatg aagaatacgg aaaatggtgg gtggatgtag aatatatttc ctggggagtt
    52261 ccgggtaaaa gccggctcat gtttgactca cgggaacagg cacaggaagt gcaggaaggt
    52321 tttcgatttt taacctgagc atattttgtt aaagggcaga aggcggtgac cctctgccct
    52381 taagtacgtt tccgggatta tcagtcaacc agttcccgga tcagttccag gagagctatc
    52441 actgcgttag ccacagcaac aaccgccctg agaacgatga ttaacaggtt aatcatccgc
    52501 ttctccatca atggagaaag aacaccgtgg tttgccctta cattgcctgt cgccacgttt
    52561 tctatagtgt cagttataga cagttcttct ccggccagag caccaaaacc agcaccacct
    52621 gatgttttgg ccaggacttg caacctgttc atcccggtga acgatatgcc ggaaagaacg
    52681 tgcctcaaag ctgacacacc gagtatatac ttatactgtg cttgcagaaa tattatttca
    52741 gccgtattat ttcatagtca gttataggca gttatcttcc ggtcagggct accaacttcg
    52801 atccggaatc gcaaaaaaac cggcctcacc agtcggtttt tttgcgtctg tacctcctcc
    52861 ccggacagca gttctctcac tcccgttgaa tatttcctgt tgaattttac gaaaatacgg
    52921 caaatgggag atatcgaata tatttacttg ggtgtagtcg gtaaaaacca gctcatgttt
    52981 gactcacaga aacaggctct ggaagtacag aaagtaatat gtccgtagat tattaaaggg
    53041 cagaaggcgg tgaccctctg cccttaagta cgtttccggg attatcagtc aaccagttcc
    53101 cggatcagtt ccaggattgc tatcactgcg ttagccacag caacaaccgc cctgagaacg
    53161 atgattaaca ggttaattgt cacgaacggt gcaatagtga tccacaccca acgcctgaaa
    53221 tcagatccag ggggtaatct gctctcctga ttcaggagag cttatggtca cttttgagac
    53281 agttatggaa attaaaatcc tgcacaagca gggaatgagt agccgggcga ttgccagaga
    53341 actggggatc tcccgcaata ccgttaaacg ttatttgcag gcaaaatctg agccgccaaa
    53401 atatacgccg cgacctgctg ttgcttcact cctggatgaa taccgggatt atattcgtca
    53461 acgcatcgcc gatgctcatc cttacaaaat cccggcaacg gtaatcgctc gcgagatcag
    53521 agaccaggga tatcgtggcg gaatgaccat tctcagggca ttcattcgtt ctctctcggt
    53581 tcctcaggag caggagcctg ccgttcggtt cgaaactgaa cccggacgac agatgcaggt
    53641 tgactggggc actatgcgta atggtcgctc accgcttcac gtgttcgttg ctgttctcgg
    53701 atacagccga atgctgtaca tcgaattcac tgacaatatg cgttatgaca cgctggagac
    53761 ctgccatcgt aatgcgttcc gcttctttgg tggtgtgccg cgcgaagtgt tgtatgacaa
    53821 tatgaaaact gtggttctgc aacgtgacgc atatcagacc ggtcagcacc ggttccatcc
    53881 ttcgctgtgg cagttcggca aggagatggg cttctctccc cgactgtgtc gccccttcag
    53941 ggcacagact aaaggtaagg tggaacggat ggtgcagtac acccgtaaca gtttttacat
    54001 cccactaatg actcgcctgc gcccgatggg gatcactgtc gatgttgaaa cagccaaccg
    54061 ccacggtctg cgctggctgc acgatgtcgc taaccaacga aagcatgaaa caatccaggc
    54121 acgtccctgc gatcgctggc tcgaagagca gcagtccatg ctggcactgc ctccggagaa
    54181 aaaagagtat gacgtgcatc ttgatgaaaa tctggtgaac ttcgacaaac accccctgca
    54241 tcatccactc tccatctacg actcattctg cagaggagtg gcgtgatgat ggaactgcaa
    54301 catcaacgac tgatggtgct cgccgggcag ttgcaactgg aaagccttat aagcgcagcg
    54361 cctgcgctgt cacaacaggc agtagaccag gaatggagtt atatggactt cctggagcat
    54421 ctgcttcatg aagaaaaact ggcacgtcat caacgtaaac aggcgatgta tacccgaatg
    54481 gcagccttcc cggcggtgaa aacgttcgaa gagtatgact tcacattcgc caccggagca
    54541 ccgcagaagc aactccagtc gttacgctca ctcagcttca tagaacgtaa tgaaaatatc
    54601 gtattactgg ggccatcagg tgtggggaaa acccatctgg caatagcgat gggctatgaa
    54661 gcagtccgtg caggtatcaa agttcgcttc acaacagcag cagatctgtt acttcagtta
    54721 tctacggcac aacgtcaggg ccgttataaa acgacgcttc agcgtggagt aatggccccc
    54781 cgcctgctca tcattgatga aataggctat ctgccgttca gtcaggaaga agcaaagctg
    54841 ttcttccagg tcatcgctaa acgttacgaa aagagcgcaa tgatcctgac atccaatctg
    54901 ccgttcgggc agtgggatca aacgttcgcc ggtgatgcag cactgacctc agcgatgctg
    54961 gaccgtatct tacaccactc acatgtcgtt caaatcaaag gagaaagcta tcgactcaga
    55021 cagaaacgaa aggccggggt tatagcagaa gctaatcctg agtaaaacgg tggatcaata
    55081 ttgggccgtt ggtggagata taagtggatc acttttcatc cgtcgttgac agttaatcat
    55141 ccgtttctcc atcaatggag gaagtcaccg ttgcttgctc atacatcgcc tgtcacaacg
    55201 gctctctgac attgttggat gtagagtaca ttcccccctg ccagagcact gaacacagca
    55261 ccacctgttt gttcagccag gactttaatt catccataca cagatagaac aggttttaga
    55321 agcaagggag gcacactgta cagcgccaac acaaagatta tacagatgca cacataaaat
    55381 tcatttaaag aaatgaatat tgacaggcaa tctgaagtca aactagacaa cagatgtctg
    55441 gtgccataat attatggatg ttggatgtag agtatcttcc tccggtcaga gcaccaactc
    55501 cgctccggaa tcacaaaaaa ccggcctcac cagtcggttt ttttgtacct gtctcccacc
    55561 accttctgcc gttcgctctt ctctgtgaaa atgtcctgtt gaactcattc ctgtccggtt
    55621 attaccggcg ctgattcgca ttcatgccac ccgacttaat cccgaaattt atccattcat
    55681 ctatggagga atccccgtgt cttaccaaca gacgtctgcc gccgaagatc caatggctat
    55741 atggtatatc gtcggtgcca tatgcctgct ctttgcgatt atcatctggc gctttttacc
    55801 tgaaatcgtc ttcgcctcct gcctgatcct gcacacactg tggggcatga ttgactgggg
    55861 gccctttcac aactttgccg ccccccgtta caacctgctg gccatcactg ctaacaacgc
    55921 ggcaaccatc actttcagcc agtggctgga tgttatgtcc cggacagtgg ggatcctctg
    55981 gttgattctg cttcccatga cattcggctt tctgtggatg tggtttcacc atccggccca
    56041 gccccgtttt acccgcagac cactgaatat ccacacgctg ccacatctgt tttcatcact
    56101 gtcaccggcg atcgcccccg ttctggctga cggagacagc aacagactct tccacggaca
    56161 aaaacgtccc gagcgccgtg tcgctctaac ccccgaagcc tttgtggagc agaacaacct
    56221 tatccgcaac atgcagctcg atgttgcggc cacgcgccag tgctttatgg cgcaactggg
    56281 ccagcctctg accacctgga aagacatggc tccacatgaa aaagcactct ttgccgtctt
    56341 tggcctgcag ttcttccttg gggaccggaa atcggctgtt tccctgatga ataacctcaa
    56401 cctctcctgc agactcaaaa gcaagcggga ccaggggcgt ttcagcacgc cagtgtattc
    56461 actggccaga aacgccttta tccgggtcat caaaacagaa ggcgcacaac agtggctcag
    56521 gcagcaccgt tacgttcgca gcggtctggt ctggctttac gcacatgatt tgcgcctgac
    56581 gcctccgaac tggctgtggc tcaagggcgt tgaccggacg ctcttctatg cccttcaccg
    56641 ggcaaacacc acaaaaggct tcatcgaagg ggccggcgtg gtggccgttg cccgggcaga
    56701 aaatgaagcc agccgtctcg gcctcccctg cccggaaccc tgtgtggaag aggccattga
    56761 agggcttcga cgggacatgc tggggctggg cctgatatgg gacgaacctc agccagatcg
    56821 cgatcgtaaa cgtcagatca ggacccgctg gtcactcacg gatgacgtga taccccgccg
    56881 ccatgataac gacgaagaca ctgacactgg tgaaaccacc gaaaccaccg aaaccaccga
    56941 aaccaccgaa accaccgaaa aacagcatcc cgccgacaag gaaaaagaac aatgaccatc
    57001 cactacactg caacactgaa taataaacac ctgtccctga tcgccctgta ccctgacggt
    57061 aatcgggagg tgattgccat cctgccagtc acagaactgg gcgcactgat taaccgtaat
    57121 aacaggctga tgcacctgtc cttcagttcc ccttttcgcg aaataaccga aacggtgctt
    57181 gttgataccg ttgatgcaga acgtcccgga catcacatcg gcagaatgct ctcagcgatt
    57241 gatgcctgcc tggatgccga atatgccgga aaaggaaaac atccctcccg gaaaaatggc
    57301 ttctggcgga cactgatgtg tggctttatc gccggaattg ctctgccggc gacagttgca
    57361 ggttaccact ggatgaaggc agagccccct tccacgctgg cttaccctac atctcaggaa
    57421 tggagagctg aaccagtaac cgacacagag acaccacgcc cacatacgga acatcatgtt
    57481 aacagcatcc cggcccgccc ggctcccaca cagcaggaac cttcttctgc ccccgccccg
    57541 acggcagtga cggaagaaca acacgttcag gccgctgagg cccgccgtaa tctggccaca
    57601 gtacttaaac gcaacgcaga ccgggggatg ttcaccatta acctgtccac tggtcatgaa
    57661 cgcacgctgt acgccttcct tgaccccgca tgcatcaact gccgtctgct ggaacccgcg
    57721 cttaagcaac tggcccggga attcagcgtg gtgatttatc cggtctccgt gattggcggg
    57781 gagtccagta caaagcggac tgctccgctg ctgtgtgaac cggactcaca aaaacgggcg
    57841 accggatggc atcagctgta ttccgctgac agtggtatgg cccccaccgg cgatgcaccg
    57901 gccgcacagg atggacagtg tctgaccgcc gcacgtgctg ccatcgatgt caataaccag
    57961 gctttccgcc agttcggttt tggcggaacc ccctgggtgt tatcagatac cggctggcac
    58021 ctgccgtccg gcatcctgaa agacgccact tccgttcgcc tcttcctgaa agcaacagat
    58081 agcgaggagt atcacaatga gccagcacca cgttaatcct gacctcatac accgcacggc
    58141 ctggggaaac ccgttgtgga atgcactgca taacctgaac atcactggtc tgtgtctggc
    58201 cggcagtatc attacggcgc tgatatggcc actggccctt cctgtctgcc tgctgttcac
    58261 cctggtcacc tccgtgatat tcatgctgca acgctggcga tgccccctgc gtatgcccat
    58321 gacactcagc cttgatgacc cctcccagga cagaaaggtc aggagaagcc tcttcagctt
    58381 ctggcccacc ctgtttcagt atgaagcgga tgaaacattc cccgcccggg ggatttttta
    58441 tgtcggttac cgccgtatca acgatatcgg tcgtgagctc tggctgagca tggatgacct
    58501 cacccggcat gtcatgtttt ttgccaccac cggtggcggt aaaacagaaa ccacctttgc
    58561 ctggctgctc aacccgctct gctggggaag aggctttacc tttgttgacg gaaaggcaca
    58621 gaacgatacc acgaggacaa tctggtatct ctcccgacgt tttggcaggg aggatgatat
    58681 cgaagtcatc aacttcatga acggcggaaa atcccgcagt gaaatcattc agagcggcga
    58741 aaaatcccgc ccacagtcaa acacctggaa cccgttcgca ttcagcaccg aagccttcac
    58801 cgccgaaacc atgcagtcca tgctgcccca gaacgttcag ggcggtgaat ggcagtcacg
    58861 ggcgatagcc atgaacaagg ccctggtctt tggcacaaaa ttctggtgcg tccgggaagg
    58921 taaaaccatg tccctgcaga tgttacggga acatatgacg ctggaaggga tggcaaaact
    58981 ctattgccgt ggccttgatg accagtggcc ggaagaagct atcgcaccac tgcgaaacta
    59041 cctgcaggat gtcccgggat tcgatatgtc actggtgaga acgccgtcag cctggactga
    59101 ggagccccgt aaacagcatg cttatctctc cggtcagttc tcggaaacgt tcacaacctt
    59161 tgccgaaacc ttcggggacg tctttgcggc agatgccggc gacatcgata ttcgcgacag
    59221 tattcacagc gaccgcattc tgattgtgat gatccctgca ctggacacat cggctcatac
    59281 cacgtcggca ctcggtcgta tgttcgtcac ccagcagagc atgctcctcg cccgtgacct
    59341 gggctaccgg ctggaaggca cagatgcaca gaccctggaa gtcaaaaaat acaaaggcag
    59401 cttcccctat atctgtatcc ttgacgaagt cggtgcctac tacacagacc gcattgctgt
    59461 ggaggccacc caggtacgtt cactggagtt ttccctgata atgacggcgc aggatcagga
    59521 gcgaattgaa gggcagacct ccgccaccaa caccgccaca ctgatgcaga acgccggcac
    59581 caaattcgcc ggacgcattg tcagtgacga caaaacggca agaacggtga aaaatgccgc
    59641 cggtgaagag gcacgggcca gaatgggcag cctgcagcgc cacgatggtg tgatgggtga
    59701 gtcctgggtg gacggtaacc agatcaccat ccagatggaa agcaaaatcg acgtgcagga
    59761 tttgatcaga ctgaatgctg gtgagttctt taccgttttc cagggggatg tggtgccgtc
    59821 agcctctgtg tatattccgg acagcgagaa aagttgcgac agtgatccgg tggtcatcaa
    59881 ccgctatatt tccgttgaag ccccccggct ggagcgtctc cgccgtctcg tccccaggac
    59941 agtgcagcgc aggctcccca caccggagca tgtcagcagc atcatcggtg tactgacagc
    60001 aaaaccatcc cgtaaacgtc gtaaaaaccg tacagagcct taccgtatca ttgatacatt
    60061 ccagcgccag ttggcaacag cgcagacatc actggatctg cttccgcaat atgatacaga
    60121 tatcgaatcc cgggcaaacg aactgtggaa aaaggctgtg cacactatca ataacacaac
    60181 cagggaagaa cgccgggtat gttatatcac actgaatcgt ccggaagagg aacactccgg
    60241 tcctgaagat ataccttcag tgaaggcgat tctgaatact ctgctccccc tggagatgct
    60301 gttacctgta ccggatctaa ctgcatcccc gccccataaa aaaaatgtcg ctcagacacc
    60361 atcaggtgga aaacaagaga gtcgtaaaaa aagattctga taacacctgt atggctgatt
    60421 ttaataacaa cctttacgcg attattaatc tcctccgctc accatagtaa gcggaggatt
    60481 caggatttca atccatcacc agaggtacaa taaatgtgta ttagtagccc ggaaacaaac
    60541 tcatggtctg ttatttatcg taaaaattcc ggagaagata tcaatatcac ttccctgacg
    60601 tttaaaaata gtcttctggc agcccggaca ctgatggtgc ccgaaaacta tatgatatgc
    60661 atactcagaa acggggagag ggtcagacga tgggacagag aaatacttgc aggctcaaac
    60721 agatggtaca agtgttctcc cgataatttc gaaatactgg gtaagctccc cataataaat
    60781 aaagtcacaa aactgataaa atcataatct acggatttta atcagttaag ctaaatcagg
    60841 atgaacgctt tgattatgaa ttatacatga aaacaaatat gggcagcaca aaaaatggtg
    60901 ccagggttcc ccacgttcgt aatagattca cttctatgga tattggtatg aattcccaga
    60961 tcccactcta ttgtaaacaa gacattttta tcttttatat tcaatggctt attttcctgc
    61021 taattggtaa taccatgaaa aataccatgc tcagaaaagg cttaacaata ttttgaaaaa
    61081 ttgcctactg agcgctgccg cacagctcca taggccgctt tcctggcttt gcttccagat
    61141 gtatgctctt ctgctcctgc agctaatgga tcaccgcaaa caggttactc gcctggggat
    61201 tccctttcga cccgagcatc cgtatgatac tcatgctcga ttattattat tatagaagcc
    61261 cccatgaata aatcgctcat cattttcggc atcgtcaaca taacctcgga cagtttctcc
    61321 gatggaggcc ggtatctggc gccagacgca gccattgcgc aggcgcgtaa gctgatggcc
    61381 gagggggcag atgtgatcga cctcggtccg gcatccagca atcccgacgc cgcgcctgtt
    61441 tcgtccgaca cagaaatcgc gcgtatcgcg ccggtgctgg acgcgctcaa ggcagatggc
    61501 attcccgtct cgctcgacag ttatcaaccc gcgacgcaag cctatgcctt gtcgcgtggt
    61561 gtggcctatc tcaatgatat tcgcggtttt ccagacgctg cgttctatcc gcaattggcg
    61621 aaatcatctg ccaaactcgt cgttatgcat tcggtgcaag acgggcaggc agatcggcgc
    61681 gaggcacccg ctggcgacat catggatcac attgcggcgt tctttgacgc gcgcatcgcg
    61741 gcgctgacgg gtgccggtat caaacgcaac cgccttgtcc ttgatcccgg catggggttt
    61801 tttctggggg ctgctcccga aacctcgctc tcggtgctgg cgcggttcga tgaattgcgg
    61861 ctgcgcttcg atttgccggt gcttctgtct gtttcgcgca aatcctttct gcgcgcgctc
    61921 acaggccgtg gtccggggga tgtcggggcc gcgacactcg ctgcagagct tgccgccgcc
    61981 gcaggtggag ctgacttcat ccgcacacac gagccgcgcc ccttgcgcga cgggctggcg
    62041 gtattggcgg cgctgaaaga aaccgcaaga attcgttaac tgcacattcg ggatatttct
    62101 ctatattcgc gcttcatcag aaaactgaag gaacctccat tgaatcgaac taatattttt
    62161 tttggtgaat cgcattctga ctggttgcct gtcagaggcg gagaatctgg tgattttgtt
    62221 tttcgacgtg gtgacgggca tgccttcgcg aaaatcgcac ctgcttcccg ccgcggtgag
    62281 ctcgctggag agcgtgaccg cctcatttgg ctcaaaggtc gaggtgtggc ttgccccgag
    62341 gtcatcaact ggcaggagga acaggagggt gcatgcttgg tgataacggc aattccggga
    62401 gtaccggcgg ctgatctgtc tggagcggat ttgctcaaag cgtggccgtc aatggggcag
    62461 caacttggcg ctgttcacag cctatcggtt gatcaatgtc cgtttgagcg caggctgtcg
    62521 cgaatgttcg gacgcgccgt tgatgtggtg tcccgcaatg ccgtcaatcc cgacttctta
    62581 ccggacgagg acaagagtac gccgcagctc gatcttttgg ctcgtgtcga acgagagcta
    62641 ccggtgcggc tcgaccaaga gcgcaccgat atggttgttt gccatggtga tccctgcatg
    62701 ccgaacttca tggtggaccc taaaactctt caatgcacgg gtctgatcga ccttgggcgg
    62761 ctcggaacag cagatcgcta tgccgatttg gcactcatga ttgctaacgc cgaagagaac
    62821 tgggcagcgc cagatgaagc agagcgcgcc ttcgctgtcc tattcaatgt attggggatc
    62881 gaagcccccg accgcgaacg ccttgccttc tatctgcgat tggaccctct gacttggggt
    62941 tgatgttcat gccgcctgtt tttcctgctc attggcacgt ttcgcaacct gttctcattg
    63001 cggacacctt ttccagcctc gtttggaaag tttcattgcc agacgggact cctgcaatcg
    63061 tcaagggatt gaaacctata gaagacattg ctgatgaact gcgcggggcc gactatctgg
    63121 tatggcgcaa tgggagggga gcagtccggt tgctcggtcg tgagaacaat ctgatgttgc
    63181 tcgaatatgc cggggagcga atgctctctc acatcgttgc cgagcacggc gactaccagg
    63241 cgaccgaaat tgcagcggaa ctaatggcga agctgtatgc cgcatctgag gaacccctgc
    63301 cttctgccct tctcccgatc cgggatcgct ttgcagcttt gtttcagcgg gcgcgcgatg
    63361 atcaaaacgc aggttgtcaa actgactacg tccacgcggc gattatagcc gatcaaatga
    63421 tgagcaatgc ctcggaactg cgtgggctac atggcgatct gcatcatgaa aacatcatgt
    63481 tctccagtcg cggctggctg gtgatagatc ccgtcggtct ggtcggtgaa gtgggctttg
    63541 gcgccgccaa tatgttctac gatccggctg acagagacga cctttgtctc gatcctagac
    63601 gcattgcaca gatggcggac gcattctctc gtgcgctgga cgtcgatccg cgtcgcctgc
    63661 tcgaccaggc gtacgcttat gggtgccttt ccgcagcttg gaacgcggat ggagaagagg
    63721 agcaacgcga tctagctatc gcggccgcga tcaagcaggt gcgacagacg tcatactaga
    63781 tatcaagcga cttctcctat cccctgggaa cacatcaatc tcaccggaga atatcgctgg
    63841 ccaaagcctt agcgtaggat tccgcccctt cccgcaaacg accccaaaca ggaaacgcag
    63901 ctgaaacggg aagctcaaca cccactgacg catgggttgt tcaggcagta cttcatcaac
    63961 cagcaaggcg gcactttcgg ccatccgccg cgccccacag ctcgggcaga aaccgcgacg
    64021 cttacagctg aaagcgacca ggtgctcggc gtggcaagac tcgcagcgaa cccgtagaaa
    64081 gccatgctcc agccgcccgc attggagaaa ttcttcaaat tcccgttgca catagcccgg
    64141 caattccttt tcctgccaga catgctgatt aattttgccc ttattaacat cacagacaga
    64201 aagaacgagg gtaccaatac cattgatggt aactggcagg ccgatgaagg acgccgttac
    64261 cgggataatg taagaatcta tttttgagta tgtcaacata acctgaggca aagtacctca
    64321 ggttccagtg tagtaatcag ccaacctgac agtcttctgt tagccctaca gatatgtcgt
    64381 ggagctaacc gcgcatgtca aagccggatt tttgaaaatc tctataaact tccggattgt
    64441 tgaaggccgg gaatttagct ttatgggctg ctgactttat cgcttcgcaa taggctttat
    64501 cgccattact ggttgatatt tttaacgccg tgccatcccg ggagaattcc atatgcaacc
    64561 tgcatttttt ccttttccat ttttgcggat catcaagttt agcattaatt gcatccctga
    64621 ttccccgtgc ttgctccccc cattcatcct gatcatccca acgtcctgaa ctgcaactac
    64681 ccagggctgt agttttatgg caatttgaag gatgtaatgg cgcacatccc gccacaaagc
    64741 tgactaaaat agccagcata acgattttct ttacccccat atattgctcc tcaatccata
    64801 tagatctcag taccatagct ctgtcagagc aaagaaaatc ttaactaata caagttaaga
    64861 tttttaatat ccactcctca tccctacatt ccaggtgatg gtggcgtcca ctcattatcc
    64921 tgaactggag cataagatgc gccatgcccc ccggatatgc ttttctgttg cctgatatat
    64981 tcgtggtagt actgttcact acgctgacga tgtaaatcca catccgggcg gctgattaca
    65041 aagtgtccac ttccggttat tccagctgca ttatgccagg ccaccatttc cgcaaataca
    65101 tcattaaact gttgatatga gccttccggt tcaaaaccca ttcttggctc acgatcaaac
    65161 aatacaggag caatcagatt cagcttatcc accagttcat ttctgtcctg atgatggtag
    65221 aacaccacac ggtccccata atccacacag actaactcgc cattcctccg atcacgaaaa
    65281 cgcacactgc catctttctg cagacgggct tcgagaacgc cagtgtcttc ataaagtgga
    65341 gttcctcccg gttcacagag aatcacgcag cggtcggcat tcgttgttcg tcgcttgtct
    65401 ttgcgacgat cacgataatc ccagccacgt aactgtgata atgcagctct gtctccctgt
    65461 accgcctgtt gctccaccca ctgccggtag gacaaaggat accacttacc ctccgcaatc
    65521 agtgacaacc gctcttcctt cacactctcc ttcagcctga tcagcgcctg catacgctgc
    65581 acttctgcaa tatggtaatg cagcttacgc aattgtggat cccggaactg cacacggata
    65641 tacgccttgc gccgacggca ggcagcatga atttcccgta accgttctcc gtaacgtaag
    65701 tcgggtttac gccagtgctc cttccatgca agataacgcg cgcgcaaatc ttcccgcgca
    65761 gctgcacggg cttcacggcg ttcacgacgc agtacaggat cgcgccggaa gccgagctcc
    65821 acttcatcgc tggcagcaag ttccggattg tagcgacatt caggcttcac ccgttcaaat
    65881 atatctgcac ctgcaatctc aaatggccct gcctgcggct cagccctgga taacgtcaaa
    65941 tccgggtgga tactgctggc cttcactggt gtgagctcat gattaaacgc atccacaatc
    66001 acaagaccat gatgctgttt ctgcaacata agccctttac gcgcaaacat ctcatggcac
    66061 gcctgccagc tgaaattccc gcccgcgatc atatcttcaa tcaacgactc acactgaaca
    66121 gtcgtgtacc cccacagact ttccttatcg gaaaacagtt cgcgctttat tgctccctgt
    66181 ggacctgtcc ccatccgaat acgataccat tcgtaatcac tgacagcctg agtaccaaga
    66241 ttacgtcccc ggaatcgttc tccgggtata acctgagtaa aaatatcctt cggtacaggc
    66301 tgccatccac catccagatc ctgcatataa tccagcgtca ttaacggcca cacactgtct
    66361 gcacgtactg gtgtctgttg tttatcaaat ccatcatgca gtaccagatg cccgtgctgg
    66421 atccccatcc ataatccagc ctttgccaga gttctgtgca catccaggca actttccagc
    66481 tcaccatttc tcgccatttc cggtaagcgt tcggcaaaat gacggcaggc gtactgtttc
    66541 agactctccg tttctgccac tttttccggc cggatatcaa cgtttatggc ttccggatca
    66601 taacgaccgg gtgtccccac ctgactgaaa atatctgtcg gtacaggctg atattccccc
    66661 agtttccgcc ccagtttttc agctgtccat gacggtccga acgaactgag tgcaacacct
    66721 tcccgcgctc tgtcccagcc atccttcacc accagttccc cttcctgcat cgtgatatac
    66781 agaccatcac tggccagccg tttatgcaaa gacaaccagt cttgtgcggg ctcttcacgc
    66841 agcccggcaa tcgacatctg cgcaatatat tccctgaacg tctgcttttt cccgcgtcgc
    66901 caggcattac ggcgctcacg taccagcgct gttttgcgca caatacggtt gccgggtgca
    66961 tgcacaaagc agccattatc aggcgcgaat ccgtgcttaa gttcaagttc acgacatgca
    67021 cggctgagtt tttcctggct ccaggaaagc cagttaatat aaccagtttc aggatgtaca
    67081 cgattcacgg caacatgcac atgcagatta tccgtgtccg tatgaacggc agccacgtac
    67141 tgatgttttg agagttcaag agatttcagt gtgtgacgca cacagtcaaa aagctgctca
    67201 ggacgcgggc tttcatgcgc aggccaggaa agaatgtagt ggaaaacggg gtctgtatca
    67261 ttacgggaaa aaacagcttt gtccgccttg tactgcatct cttcagcagc tgtttccagt
    67321 gaattgcagt tatggaaaca ggttacgcca tagaaattta cccactgact gccgtccttc
    67381 atcacatcaa tcagggagac aaatttttcg ttacgcaggc gtgttgcata atcaacaagc
    67441 cgtctgaaac ggttgcggtg agggacatca ctcttgattt cgctgtcttc tgtgagctca
    67501 ttttcctgca catcatcccg cacagacgtg tacgcaatca ggtcttcaaa ggaagacctg
    67561 ccgtcacgtc ttttgtgcgg aataatggca ttcatcagca gttatctcag cgtcccatta
    67621 agtctcttcg cagagcattg acatattcag taatggcaac aagaacctct gcatactcct
    67681 tatcaccagt gcgttttccc tccacaaaca gatgcttctg aaggcggcca agacgcataa
    67741 tctcatcaat catacggtcg tcagtacggc agttaaccac cttatccagt gaagaacttc
    67801 gtataaatgt tgaaaccgtc tgtccacttg actccgcttt ttctcttacg atttcagcct
    67861 catcctccgt gaatctcgcc gtgaatttta caattctctt cctgttctca ctgcccgttc
    67921 gggttttctt ctcactcatg gtctgctcct taccggggtg tcggggcaaa gccctgacca
    67981 ggtggggaat gtctgagtgc gcgtgcgcgg tccgacattc ccacatcctg tcccgttttt
    68041 cagtccatta tagcctgtta tttttgtgca tcatagtctt atgcagacgg cagaggcagg
    68101 gcgcagccct gccaggagtc agtttttgat gataaaatca ggcttcaaca ttaaacggag
    68161 agaaagtgcc aatggcgtct aaaaaatttt actcagatga tgacatccag ctggcaaaag
    68221 ctgctttatc ggaattgcca gacctcaccg ctcaacggaa aacattgcgt gattttcttg
    68281 atgccattcg tgatgacatc attatcctgg taaggacaaa gggttataca ctgacagatg
    68341 ttcgtgacac gctcaaaaat gcaggatatg aagcaggaga gaaagcactt cgtgacatta
    68401 ttcgggaagc agaatcgaaa aaaccatccc gccgttcttc ctctaaaaca gcacctaaaa
    68461 agacagactc aggcaggaaa gatggcatcg atatgaacaa caattgatgt gacaatgtca
    68521 cattgtgttc tgagattatg atgattacag aaaaaaaagc cccggcataa atacctgggc
    68581 ttttctgttt aatcagactc gtggcggcaa tgcaccagga taatgccgtg caataatatc
    68641 attcagtttt tcaacaaggt cagaacgctt aaagacaatg tgtacggtcc ctttctggta
    68701 gccacgcacc gtaaaatatt ccccttctaa cagcttaccg acattgtcac cgttcccgag
    68761 aaattcacca tactgcgccc cgatgctgtt cctgaagtca ggaacgttgc gcccctccag
    68821 cagataaaac ggacgtacta ggtcatccag tgcctcccgt ccgctgcagt ccagcgtcac
    68881 acgcccgtta ctccagcggt acagtaggtt ttcaataatt attttcttac ccagacggca
    68941 gggattattg gttctgtaat cccatgacaa tttccggtag acatcaatca gcccctgttc
    69001 aaacgtctgc atcttgctgg cattaagatg gcggaatgtt gccagtacat tgtccagggt
    69061 gatttcagga caggtgtcgc tcatcagctg gctgttccac tcatcacgct ggcatgaact
    69121 catgaaggtg tacatgcccg tatctgtcat caggcggtcc cagattttcc ggtcggcaag
    69181 acgggtgata ttccttttcg cttcttccgt cttatccatg cgacgaacgg aatcagtcac
    69241 gcagtcttca aacccgtaaa gaaaaatatc acctgacgca tcgagcagaa tcttgcgcgc
    69301 tgtcgccagg cattccatcg cctgcatgaa cagtgcaata ccttcgttac gctgcgcgat
    69361 gatgcggtcg atagctacag acggaataac atctgtcgtg ccggcagtat tcaggtcagc
    69421 agtgggtaaa gtctgattca tcacattgtc tcctcatcgt cacgactgcc gggtcagcca
    69481 ggtcatttcc cggaggagcg aagctccttc tgccgtccgg gtcttcgggg atttccccgc
    69541 agtggacctt tccgacgggg gacgctcacc acgtcacggg gcagttgtcc gggggcagac
    69601 gcaaagtttt tgacgcctgc ggcaaaaagt tttgtggctg gtacatctct cccctggacg
    69661 actgaaacgg gatgtggtca tgtatgtacc cccggaaagt ccgtctgcgg agaacaggcc
    69721 gcagtgcggc ggcagacagg cagagacagc tgcgccatca ggcgcagtgg ccttacacac
    69781 cgtaaggtgt gcagggagcg ccgcatacgg cgcgactgaa gaaaaacgac ggcacaacaa
    69841 tggcatgtgg tggtcactgg cacaccactc ccgcacaggt gaaaacagag aaaaccgcac
    69901 gacatttaca accaaaaaaa agtgaaattt cttcactttt tcacgcttat ttttcgcgtt
    69961 gatttggtga ttatgatgat aaaataaagt gaaatttttt cactttttca aaggtgtttt
    70021 atgaccggct gggaactgcg aatctggcga aaaagcatgc tctggtcacg cgaaaaagcg
    70081 gccagagaat ttggcgtgac gcagagaaca tggcacgcct gggaaaatgc ggaacaggtg
    70141 gacgtcaccg tctggaggac cacccaggca ctttcagtcc gtgacctgct gcctcacatg
    70201 caggggatgc gtaaggcaga tatcatcagg cgactggaaa atgaactggg ggaaacggca
    70261 gggaatgtgt gagcgtgatt cacagcctgg ctgaatgcca ggctactgct catcaggaaa
    70321 cagctttttc agttcttcac cggaaagtcc cgtgacctcc tgaatccgtt ccagcggcca
    70381 gcctatatcc cgaagccggc aggctatttc ccgcgccact tcaagagccc cttgtttaaa
    70441 accctcgtcg aaacctttct gtataagacg ttctgcaatc gtcatgctgt ttgctctgct
    70501 cctgctgaca aaaaagcccg gcattttgcc gggctttgtg gttctcatca gtgagtgatt
    70561 ttcttcagtt cgtcatcgga aagtccggta gcctgcttaa tctgctccgg cgtcatcccc
    70621 attttctgaa gctggcacgc aatagccagg gctttttcaa gcgctccttc catgcgtcct
    70681 tccatgcgtc cttccatcct gccttcctgg cgaccttttt gctcaagacg ttctgcgata
    70741 gtcatcagtt tatcctcatg ctgcggcaga cgctcagcca gcgcccgcat aaattccggt
    70801 gatacagagt cccctgcgtt aacgatgtaa tgaatcagaa cacgcacctg ctcgtctgaa
    70861 atttccacca tgacctgcgg gagtttgtcc agcagctcca tcatgtcacg atggcggata
    70921 tgcttcatca gcagcgtcag tgcggccatc cggcgatggt tcatgatttc attgtcatca
    70981 acgacagtga tatcaaccag cggaaacggc tttgtgtata ttttagccgc aagtgccgga
    71041 ttctcaaaac agtccagcca gttcatgctg tacgggtacg ggctgcgctc accgtgatag
    71101 aacagcaccg gaatcaccag tggcaacgtt ttgtgctgct ccagatgtct ctgcatcgcc
    71161 gccacactgt agcgtaacat gcgaaaaggc atgtacagat tttctgtgct ctggtgctcg
    71221 atgagcgtat aaacataacc atcttcaccc ccggtggttt tcacgctcca gaggatatcg
    71281 ctggcgtact gatgcaggtc cggctcaaca aaggtggcgg gttccagctt cagcgtggac
    71341 aggtcgcaca actgccggta ctccgcagga agatgcagtt ccagaaaatc cctggcaacg
    71401 tcgggattcg ccaggaacga ccggaacgcg gcatcatgcg gcgtgggcgt tgtggtgttt
    71461 ttcttcttac tcacggttcc tccatcaaat cagcagcatc gcagcaatca tcgcccaaat
    71521 ccccacaacc gagaggacag caattgcgtt caaagccatg acaacaatag ccgcgttctg
    71581 agtcacggcg tatttcttca gcaatctctt ctgcggaaag gtaatcaaac tcctcacggg
    71641 ccagttccag gaccagtaga tgttgttctg tcggcaatgt ggccacaaaa ttatcaatag
    71701 cagcactcaa acgtcgctga tatagctcat aaacaagacc acaacggggc attagttctt
    71761 cggataatag ttccagtttt gagcgaattc tgtcagcgtt gaaagtgact tcatcaaaag
    71821 acatatgaaa agctttgtca gtatcaaaac aagacttcat tgttatctcc agccctcccg
    71881 ggccctgaat gaaagagaat ctgtttccct tttctgtttt tttgtggaga caccatgcaa
    71941 cacagacgtc gtgatgtttt tcatcttgct cacggttcct cctggtaaag tacctgaaca
    72001 catcctgcca cctgcacagg cagacggcag gggttaatca gtgacagacc cgttcaattt
    72061 caagcgcgcg gagcaggtgg ccccattcgc tgactttcat agcggggata cgtacccgct
    72121 tctgctcact gcagcgtctg gcgatatgac cgcccatcgc caggacgtcc gggttaatct
    72181 catccggtgc cagttcctgg cgtctgaagc gggtatggcg gtgcaggggt ttgttctccc
    72241 gcaggcactc ttcacgcaga cgctttacat actcggcggt tgcgaagata attgatggtc
    72301 tcatggcata atctccatag ttagtgaatg agatgccgga cttccgtacg gggaagcgcc
    72361 agcggagaaa ggcagggttt atcgccctgc tttttttcat tccggacagc gacgcagtgc
    72421 cagcgcgagc gctttcatct tccagccttc cggcgtgcgg tacatcacct tccagtagcc
    72481 gtcgatgaac acataatgga agcgcacctc ttcagtgctt tcagtggcaa tagcctccct
    72541 gaaagaacgg tactcacgcg cctcactgtc catccggaaa ccactgtcac gtccgtaata
    72601 agccgtcaca ccgtccaccg ggttctgaag cgtatgcccg gcgggtttgt cgcaacgggg
    72661 accgaggcaa cggatatcac cgtgttcaac cagggcttcg gcctgctgac cggtggcata
    72721 gtgggtatgc aggatgcgac cgttccaggt cggctcaccg tgcaggtggc agtaaacggt
    72781 acggaacacc ccgtcgcggc aaaaaacgga aatcgtgctg ctgactgtca tgaaaacgtc
    72841 tccttctgct gaatcaggtc gccgggccga ccggttgctc ccccggaggg gcgaaagccc
    72901 cttctgccgt ccacttcttc ggggtttctc cccgcagtgg acctttccga tggggcagcg
    72961 acagccacgg cacggggcag ttgtccgggg gcagacgcaa aagtttttga cgcttgcggc
    73021 aaaaagtttt gtggctggta catctccccc ctggacggct gacacgtgtt gtggccgctt
    73081 cagcccccgg aaagtccaca gcgggaaaca ggccgcaggg gtggcggcgg aagggcagac
    73141 aactgcgcca tcaggcgcag tttccttaca caccgtcagg tgtgcaggga gcgcaggtca
    73201 cggcgcgaca gaggcggcgc gaccggaacg gtcgctgcaa gcctggtttt tcagacatcg
    73261 gaccgtagcg gcagcccgaa accgcgccag cgggttcggc ggagactggc gggtgcgcca
    73321 gcagctgcct gtcggagtcg ggcctgacga ccggatgccg ccaggcagcc gggagccgaa
    73381 gggtgtcccc gcccccggga gtcagaacaa cagaaacgac aaaacagaaa aatgcctcac
    73441 actatgacag gcaggaaggg cagcgccgca ggcgcggccc tcctgtattt caggcaggcg
    73501 taagcctgcc agggttttcc gggggcctgg caggccatac ccccgccgga gcgggggagt
    73561 aaatcaccga cggaacacat gaccgtcaat aaaggtgaag gagtccagga ataaatcccg
    73621 cgcatacgcc tcatagtcaa aatagagcgc caccgtctcc ggcacgtcag ccagcaggcc
    73681 ggtatcactg gcgaactcaa ccgcaaaagc ctcctcactg tcagcctcgc cccaccaggc
    73741 atcgcggaag gtgtcaaaat ccgtctcacc ggtatcctcc acccagagac gataagcctc
    73801 ttcgcagcct tcatcccgcg cctggcggaa gccttcaacc caggcccagt tgatatggca
    73861 ttcagaggcc atattccccg ggaatccctc ataatcctga aacatcagtt ccgggtcggt
    73921 ttcatcctgg tgaagggcac ggcaggcggc gaaaaagtcg cgttcatcat caaacgttgt
    73981 caggtcaaac cagcggccag cgatgcttcc acagttgtat ttgtgccagg ttccaacgta
    74041 tacagcaggt gcaacaacag acattcccga atcctccata gtcacatgtt c
//
LOCUS       DADMKF010000003        64591 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.003, whole genome shotgun sequence.
ACCESSION   DADMKF010000003 DADMKF010000000
VERSION     DADMKF010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 64591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64591
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.003"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(35..1333)
                     /gene="eno"
                     /locus_tag="JNC24_000166"
     CDS             complement(35..1333)
                     /gene="eno"
                     /locus_tag="JNC24_000166"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HAY9157028.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAADKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(1421..3058)
                     /gene="pyrG"
                     /locus_tag="JNC24_000167"
     CDS             complement(1421..3058)
                     /gene="pyrG"
                     /locus_tag="JNC24_000167"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase (glutamine hydrolyzing)"
                     /protein_id="HAY9157029.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(3286..4077)
                     /gene="mazG"
                     /locus_tag="JNC24_000168"
     CDS             complement(3286..4077)
                     /gene="mazG"
                     /locus_tag="JNC24_000168"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HAY9157030.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     ANEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(4148..4483)
                     /gene="mazF"
                     /locus_tag="JNC24_000169"
     CDS             complement(4148..4483)
                     /gene="mazF"
                     /locus_tag="JNC24_000169"
                     /EC_number="3.1.27.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease MazF"
                     /protein_id="HAY9157031.1"
                     /translation="MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTG
                     MCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKSIAWRARGATKKGTVAPEELQLI
                     KAKINVLIG"
     gene            complement(4483..4731)
                     /gene="mazE"
                     /locus_tag="JNC24_000170"
     CDS             complement(4483..4731)
                     /gene="mazE"
                     /locus_tag="JNC24_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MazE"
                     /protein_id="HAY9157032.1"
                     /translation="MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLII
                     EPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKEVW"
     gene            complement(4809..7043)
                     /locus_tag="JNC24_000171"
     CDS             complement(4809..7043)
                     /locus_tag="JNC24_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HAY9157033.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(7091..8392)
                     /gene="rlmD"
                     /locus_tag="JNC24_000172"
     CDS             complement(7091..8392)
                     /gene="rlmD"
                     /locus_tag="JNC24_000172"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAY9157034.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVTVTEDKKQYARAKVVRRLSDSPGRETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHEVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILVPQLEALLPKVMACLGSLQVMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            8449..11205
                     /gene="barA"
                     /locus_tag="JNC24_000173"
     CDS             8449..11205
                     /gene="barA"
                     /locus_tag="JNC24_000173"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HAY9157035.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISSVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSTQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQQ
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(11437..12777)
                     /gene="gudD"
                     /locus_tag="JNC24_000174"
     CDS             complement(11437..12777)
                     /gene="gudD"
                     /locus_tag="JNC24_000174"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HAY9157036.1"
                     /translation="MSSQFTTSVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDAGGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPYGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(12798..14138)
                     /locus_tag="JNC24_000175"
     CDS             complement(12798..14138)
                     /locus_tag="JNC24_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase-related protein"
                     /protein_id="HAY9157037.1"
                     /translation="MTTQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKRFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAMFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPGGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(14140..15492)
                     /locus_tag="JNC24_000176"
     CDS             complement(14140..15492)
                     /locus_tag="JNC24_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY9157038.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSHIVAAWFPAQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQNTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(15927..16376)
                     /locus_tag="JNC24_000177"
     CDS             complement(15927..16376)
                     /locus_tag="JNC24_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HAY9157039.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(16394..17176)
                     /gene="truC"
                     /locus_tag="JNC24_000178"
     CDS             complement(16394..17176)
                     /gene="truC"
                     /locus_tag="JNC24_000178"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HAY9157040.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(17176..17505)
                     /locus_tag="JNC24_000179"
     CDS             complement(17176..17505)
                     /locus_tag="JNC24_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HAY9157041.1"
                     /translation="MTTHDRVRLQLQTLEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWVLIPRMHDLLNNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(18127..18672)
                     /gene="syd"
                     /locus_tag="JNC24_000180"
     CDS             complement(18127..18672)
                     /gene="syd"
                     /locus_tag="JNC24_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HAY9157042.1"
                     /translation="MDDLTAQALKDFTARYYDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLASNLAEFLNQLKPLL"
     gene            18740..19588
                     /gene="queF"
                     /locus_tag="JNC24_000181"
     CDS             18740..19588
                     /gene="queF"
                     /locus_tag="JNC24_000181"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HAY9157043.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKISVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATCGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            19700..21064
                     /gene="ppnN"
                     /locus_tag="JNC24_000182"
     CDS             19700..21064
                     /gene="ppnN"
                     /locus_tag="JNC24_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase"
                     /protein_id="HAY9157044.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLFLTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
     gene            21620..22909
                     /locus_tag="JNC24_000183"
     CDS             21620..22909
                     /locus_tag="JNC24_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HAY9157045.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
     gene            22967..24334
                     /gene="sdaB"
                     /locus_tag="JNC24_000184"
     CDS             22967..24334
                     /gene="sdaB"
                     /locus_tag="JNC24_000184"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HAY9157046.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     NSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            24356..25201
                     /gene="xni"
                     /locus_tag="JNC24_000185"
     CDS             24356..25201
                     /gene="xni"
                     /locus_tag="JNC24_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311685.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HAY9157047.1"
                     /translation="MRGLFPISHPAVACSGIECYPYRLIFKGVIVAVHLLIVDALNLI
                     RRIHAVQGSPCVETCQHALDQLIMHSQPTHAVAVFDDENRSSGWRHQRLPDYKAGRPP
                     MPEELHDEMPALRAAFEQRGVPCWSTSGNEADDLAATLAVKVTQAGHQATIVSTDKGY
                     CQLLSPTLRIRDYFQKRWLDAPFIDKEFGVQPQQLPDYWGLAGISSSKVPGVAGIGPK
                     SATQLLVEFQSLEGIYENLDAVAEKWRKKLETHKEMAFLCRDIARLQTDLHIDGNLQQ
                     LRLVR"
     gene            complement(25306..26454)
                     /gene="fucO"
                     /locus_tag="JNC24_000186"
     CDS             complement(25306..26454)
                     /gene="fucO"
                     /locus_tag="JNC24_000186"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HAY9157048.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMHYNADFTGEKYRDIARVMGVKVEGMSLEEARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDIPALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(26482..27129)
                     /gene="fucA"
                     /locus_tag="JNC24_000187"
     CDS             complement(26482..27129)
                     /gene="fucA"
                     /locus_tag="JNC24_000187"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HAY9157049.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRPIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYVATLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            27676..28992
                     /gene="fucP"
                     /locus_tag="JNC24_000188"
     CDS             27676..28992
                     /gene="fucP"
                     /locus_tag="JNC24_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HAY9157050.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSGHFRLNLAQT
                     FNSFGAIIAVVFGQRLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPTLCFAVIFIFARFRSQTATN"
     gene            29025..30800
                     /gene="fucI"
                     /locus_tag="JNC24_000189"
                     /pseudo
     CDS             29025..30800
                     /gene="fucI"
                     /locus_tag="JNC24_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
     gene            30909..32327
                     /gene="fucK"
                     /locus_tag="JNC24_000190"
     CDS             30909..32327
                     /gene="fucK"
                     /locus_tag="JNC24_000190"
                     /EC_number="2.7.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HAY9157051.1"
                     /translation="MKQEVILVLDCGATNVRAIAVNRQGKIVARASTPNASDIAMENN
                     TWHQWSLDAILQRFADCCRQINSELTECHIRGIAVTTFGVDGALVDKQGNLLYPIISW
                     KCPRTAAVMDNIEQLISAQRLQAISGVGAFSFNTLYKLVWLKENHPQLLERAHAWLFI
                     SSLINHRLTGEFTTDITMAGTSQMLDIQQRDFSPQILQATGIPRRLFPRLVEAGEQIG
                     TLQNSAAAMLGLPVGIPVISAGHDTQFALFGAGADQNEPVLSSGTWEILMVRSAQVDT
                     SLLSQYADSTCELDSQAGLYNPGMQWLASGVLEWVRKLFWTAETPWQMLIEEARLIAP
                     GADGVKMQCDLLSCQNAGWQGVTLNTTRGHFYRAALEGLTAQLQRNLQMLEKIGHFKA
                     SELLLVGGGSRNTLWNQIKANMLDIPVKVLDDAETTVAGAALFGWYGVGEFNSPEEAR
                     AQIHYQYRYFYPQTEPEFIEEV"
     gene            32329..32751
                     /gene="fucU"
                     /locus_tag="JNC24_000191"
     CDS             32329..32751
                     /gene="fucU"
                     /locus_tag="JNC24_000191"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HAY9157052.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            32809..33540
                     /gene="fucR"
                     /locus_tag="JNC24_000192"
     CDS             32809..33540
                     /gene="fucR"
                     /locus_tag="JNC24_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HAY9157053.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(33584..34684)
                     /gene="rlmM"
                     /locus_tag="JNC24_000193"
     CDS             complement(33584..34684)
                     /gene="rlmM"
                     /locus_tag="JNC24_000193"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HAY9157054.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVDMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPAEAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(34677..35072)
                     /locus_tag="JNC24_000194"
     CDS             complement(34677..35072)
                     /locus_tag="JNC24_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HAY9157055.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            complement(35091..36008)
                     /locus_tag="JNC24_000195"
     CDS             complement(35091..36008)
                     /locus_tag="JNC24_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GcvA"
                     /protein_id="HAY9157056.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            complement(36359..36586)
                     /locus_tag="JNC24_000196"
     CDS             complement(36359..36586)
                     /locus_tag="JNC24_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HAY9157057.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELDQ"
     gene            36778..37983
                     /gene="csdA"
                     /locus_tag="JNC24_000197"
     CDS             36778..37983
                     /gene="csdA"
                     /locus_tag="JNC24_000197"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HAY9157058.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTGAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMIHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEDALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            37983..38426
                     /gene="csdE"
                     /locus_tag="JNC24_000198"
     CDS             37983..38426
                     /gene="csdE"
                     /locus_tag="JNC24_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HAY9157059.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLSQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVTENGKMHFFGDSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAATKQV"
     gene            complement(38477..39283)
                     /gene="tcdA"
                     /locus_tag="JNC24_000199"
     CDS             complement(38477..39283)
                     /gene="tcdA"
                     /locus_tag="JNC24_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HAY9157060.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSVGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(39360..40457)
                     /gene="mltA"
                     /locus_tag="JNC24_000200"
     CDS             complement(39360..40457)
                     /gene="mltA"
                     /locus_tag="JNC24_000200"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HAY9157061.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LSDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            40666..40742
                     /locus_tag="JNC24_000201"
     tRNA            40666..40742
                     /locus_tag="JNC24_000201"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:40700..40702,aa:Met,seq:cat)
     gene            complement(40817..42070)
                     /locus_tag="JNC24_000202"
     CDS             complement(40817..42070)
                     /locus_tag="JNC24_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMIN domain-containing protein"
                     /protein_id="HAY9157062.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            42302..43633
                     /gene="argA"
                     /locus_tag="JNC24_000203"
     CDS             42302..43633
                     /gene="argA"
                     /locus_tag="JNC24_000203"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HAY9157063.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IRRQLDSGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQLDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(43695..45521)
                     /gene="recD"
                     /locus_tag="JNC24_000204"
     CDS             complement(43695..45521)
                     /gene="recD"
                     /locus_tag="JNC24_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HAY9157064.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASHPLLATCVSEIGELQNWEESLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHAIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERAGQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHSHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPIVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRG"
     gene            complement(45521..49063)
                     /gene="recB"
                     /locus_tag="JNC24_000205"
     CDS             complement(45521..49063)
                     /gene="recB"
                     /locus_tag="JNC24_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HAY9157065.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLTVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYEHLLE
                     EVDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIDKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLVITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDRHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            complement(49056..51944)
                     /gene="ptrA"
                     /locus_tag="JNC24_000206"
     CDS             complement(49056..51944)
                     /gene="ptrA"
                     /locus_tag="JNC24_000206"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HAY9157066.1"
                     /translation="MPRSTWFKALLLLVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPAMPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISEQTFADWQQKAANIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFSSEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYTQRLPQLFQALLEGY
                     FSYTATEDQLEQAKSWYNQMMDSAEKGKAFEQVIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPDEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(52120..55488)
                     /gene="recC"
                     /locus_tag="JNC24_000207"
     CDS             complement(52120..55488)
                     /gene="recC"
                     /locus_tag="JNC24_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HAY9157067.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFSIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHQLGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEDPTLTPRDIIVMVADIDSYSPFIRAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLSAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(55501..55824)
                     /locus_tag="JNC24_000208"
     CDS             complement(55501..55824)
                     /locus_tag="JNC24_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HAY9157068.1"
                     /translation="MSASLKNQQGFSLPEVMVAMVLMVMIVTALSGIQRTLMNSLASR
                     NQYQQLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGGREGEMTRLH
                     CPNRQ"
     gene            complement(55809..56216)
                     /locus_tag="JNC24_000209"
     CDS             complement(55809..56216)
                     /locus_tag="JNC24_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HAY9157069.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSIESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAGTDARVCLRLLADNEALLVAGYEGVSL
                     WRTGEVIDGNIVFSPRGWSDFCPLKERALCQLP"
     gene            complement(56213..56776)
                     /locus_tag="JNC24_000210"
     CDS             complement(56213..56776)
                     /locus_tag="JNC24_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HAY9157070.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            complement(56767..57237)
                     /locus_tag="JNC24_000211"
     CDS             complement(56767..57237)
                     /locus_tag="JNC24_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HAY9157071.1"
                     /translation="MKTQRGYTLIETLVAMLILVMLSASGLYGWQYWQQSQRLWQTAS
                     QARDYLLYLREDANWHNRDHSISVIREGTLWCLVSSAAGANTCHGSSPLVFVPRWPEV
                     EMSDLTPSLAFFGLRNTAWAGHIRFKNSTGEWWLVVSPWGRLRLCQQGETEGCL"
     gene            complement(57422..58216)
                     /gene="thyA"
                     /locus_tag="JNC24_000212"
     CDS             complement(57422..58216)
                     /gene="thyA"
                     /locus_tag="JNC24_000212"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAY9157072.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAPVAI"
     gene            complement(58223..59098)
                     /gene="lgt"
                     /locus_tag="JNC24_000213"
     CDS             complement(58223..59098)
                     /gene="lgt"
                     /locus_tag="JNC24_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HAY9157073.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(59249..61495)
                     /gene="ptsP"
                     /locus_tag="JNC24_000214"
     CDS             complement(59249..61495)
                     /gene="ptsP"
                     /locus_tag="JNC24_000214"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HAY9157074.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDYAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(61508..62038)
                     /gene="rppH"
                     /locus_tag="JNC24_000215"
     CDS             complement(61508..62038)
                     /gene="rppH"
                     /locus_tag="JNC24_000215"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HAY9157075.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNTSAYRRKRG"
     gene            62723..63412
                     /gene="mutH"
                     /locus_tag="JNC24_000216"
     CDS             62723..63412
                     /gene="mutH"
                     /locus_tag="JNC24_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HAY9157076.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            63481..64194
                     /locus_tag="JNC24_000217"
     CDS             63481..64194
                     /locus_tag="JNC24_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HAY9157077.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASIAWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            64332..64550
                     /gene="ygdR"
                     /locus_tag="JNC24_000218"
     CDS             64332..64550
                     /gene="ygdR"
                     /locus_tag="JNC24_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HAY9157078.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
BASE COUNT        15652 a        17365 c        15825 g        15749 t
ORIGIN      
        1 gaggctggca tttttaaatc agataaagtc agtcttatgc ttggcctttg atctctttac
       61 gaccgttgta cggtgctttt tcgcccagag cttcttcgat acgaatcagc tggttgtatt
      121 tagcaacacg gtcagaacgg ctcatagaac cagttttgat ctggcctgca gcagtaccaa
      181 cagccaggtc agcgatggta gcgtcttcag tttcgccaga acggtgagag ataactgcag
      241 tgtagccagc atctttcgcc atcttgattg cagccagagt ttcggtcaga gaaccgatct
      301 ggttgaattt gatcaggatg gagttagcga tacctttttc gataccttct ttcaggatct
      361 tggtgttggt tacgaacagg tcgtcaccaa ccagctggat tttgtcgccc agaactttgg
      421 tctggtatgc gaaaccgtcc cagtcagatt cgtccagacc gtcttcgata gatacgatcg
      481 gatactgttt ggtcagttct tccaggaagt gagtgaattc ttcagaggtg aaagctttgt
      541 tgccttcgcc agccagaacg tatttaccgt ctttgtagaa ttcagaagct gcgcagtcca
      601 tcgccagagt gatgtctttg cccagttcat aaccagcagc tttaacagct tcagcgataa
      661 cagccagagc ttcagcgttg gaacccaggt tcggcgcata gccaccttcg tcaccaactg
      721 cagtgttcat gcctttcgct ttcagaactt ttgccaggtg atggaaaact tcagaaccca
      781 tgcggatggc ttctttcaca gttttcgcgc caaccggctg aatcatgaat tcctggatat
      841 caacgttgtt gtcagcgtgc tcaccaccgt tgatgatgtt catcatcgga accggcatgg
      901 agtatttgcc cggagtgccg ttcagttcag cgatgtgctc gtacagcggc atccctttat
      961 cggcagcagc agctttggcg ttagccagag atacagccag gattgcgttc gcgccgaatt
     1021 tggatttgtt ttcggtgccg tccaggtcga tcatgatctt gtcaatgcca gcctgatctt
     1081 tagcgtcttt gccaatcagt gcctgagcga tcgggccgtt taccgcagca acagctttgg
     1141 ttacgccttt acccaggaaa cgggatttgt cgccatcgcg cagttccaga gcttcacggg
     1201 aaccagtaga agcacctgac ggagcagctg ccataccgac gaaaccaccc tccagatgta
     1261 cttcggcttc aacagtcggg ttaccacggg agtcgatgat ttcacgaccg atgattttta
     1321 cgattttgga cattaggttt tcctcaagtc actagttaaa ctgaaactcc agacaaacaa
     1381 cgcgtaccca aggtacgcgt tgccgttcta acttttttac ttacttcgcc tgacgtttct
     1441 ggaactcgct ggcagctttc acaaagcctg caaacagcgg gtgaccatca cgtggagtag
     1501 aagtaaactc cggatggaac tggcaagcca cgaaccacgg gtgattcgga acttcgatga
     1561 tctcgaccaa ctgatcatcc ccggaacggc ccgcaacgcg cagacctgca tcttcaatct
     1621 gtttcaacag catgttgttg acttcgtaac ggtgacgatg acgctcaaca attgtcggcg
     1681 cattgtacag ctggcgaacc aggctatcgt caaccaactg gcactgctgt gcgccgagac
     1741 gcatggtacc gccgagatcg ctcttctcgc tacgaacttc aacgttgccg ttttcatcgc
     1801 gccactcggt aatcagcgcc acaaccgggt acttacagtc tggcacaaat tccgtagagt
     1861 tggcgttctc catgttggca acatggcgag cgtaatcaat taacgccacc tgcataccca
     1921 ggcaaatgcc cagataagga atattgttct cacgcgcaaa acgcgcggtc gtaatcatgc
     1981 cttctacgcc acgatagccg aaaccgccag gtacgaggat tgcgtccaga cctttaagga
     2041 tttcaacgcc gcgcgtttca acatcttgtg aatcgatcag tttgatgttg acgctgacac
     2101 gattcttcag cccaccgtgt ttcagtgctt cgatcactga tttataagca tccggcagtt
     2161 caatgtactt gccgaccata ccgatggtga cttcacttac cggattcgct tcttcgaaga
     2221 taacctgttc ccattcggac agatttgctt ccgggcagtt taagctgaat cgtttacaaa
     2281 tataatcgtc cagcccctga gatttcaaca ggcccggaat tttatagatg gaatcgacgt
     2341 ctttcagaga aataaccgct ttttccggaa cattacagaa caatgcaatc ttcgcacgtt
     2401 cgttcgccgg aacagcgcga tctgaacgac aaatcaggat gtcaggctgg ataccgatgg
     2461 agagcagctc ttttacagag tgctgagtcg gtttggtttt gacttcacca gacgctgcca
     2521 tgtacggcac cagcgtcagg tgcataaaca gagtgtgctc acggccaatt tcaacagcca
     2581 tctggcgaat cgcttcgagg aacggcaagg attcgatatc acctactgta ccgccgattt
     2641 ctaccagtac tacgtcatga ccttcgccac cttccagcac gcgctctttg attgcgttag
     2701 tgatgtgcgg aataacctgc acggttgcgc cgaggtagtc accgcggcgt tctttacgca
     2761 gaacgtcaga gtagatacga cccgtggtga agttgttgcg gcggctcatt ttggtacgaa
     2821 tgaaacgctc gtagtgcccc aggtccaggt cggtttcagc gccgtcttca gtaacgaaca
     2881 cttccccgtg ttggattggg ctcatagtac ctggatcgac gttgatgtac ggatccagtt
     2941 tcatgatggt cacattgagg ccacgggctt caagaatggc tgcgagggag gctgcggcaa
     3001 tgcctttacc cagagaggat acgaccccgc cggtcacaaa aatatagttc gttgtcatgc
     3061 tgaacctgag aagttaggtt gaaaagacga tggaataacc aggacgggaa agcagtatac
     3121 ccgaacatga cctgtgccac aaactttcat tatccctcct cttcgccagc gcactattga
     3181 aatcaggagt gagaaaatag cccctttggg gtaaatgttt ttgacgcaaa tcaagcgctt
     3241 gtcatttaaa aaatgacaca aatggcgctt gaccgcgtaa ttcccttaga gatcaatttc
     3301 ctgccgtttt acctgttgcc agacttcttc cattgtttcg aggtcaacac ctgtcatttc
     3361 cagtccacgc gcggcaacaa tacgctccac ttcgcgaaaa cgacgctcga atttttcgtt
     3421 cgctttttgc aatgcgattt ctgctttcgt ccctaaatgg cgagccagat taaccgtggc
     3481 aaacagcagg tcccccattt cctcctccag tttagcctgg tcgacaacag cctgccgcgc
     3541 ctcgtacatc acctcgtcga tctcttcgta gactttatca accaccgggc caagcgtcgt
     3601 ccagtcgaag ccaacgttgg cgcaacgttt ctggattttt tgcgcccgca ttaaagccgg
     3661 taaactacga ggaatatcgt ccagcgccga atgctgcgct ttctgcgcgc gctcttcggt
     3721 tttgatttgc tcccaacggg caagcacttc actactgttt tcggcagaac tatcagcaaa
     3781 aacatgcgga tggcgacgct ctaatttatc gctaatagca gcgcaaatat cattaaagtc
     3841 aaagcgccct tcttcctgag ccatttgcgc gtaaaacacc acctggaata gcagatcgcc
     3901 cagttcaccg cgaagatcgt caaaatcttc acgggcgatg gcgtccagca cttcgtaggt
     3961 ttcttcaagg gtgtaaggcg caatggtggc aaatgtctgc tctttatccc acgggcagcc
     4021 gttttccgga tcgcgcaggc gctgcataat agtgagcaaa cggtcgattt gattcattga
     4081 attgtcctga aaattgcggg tctgtcaggt ggaaacctgt gaccagaata gaagtgagtt
     4141 agtaacacta cccaatcagt acgttaattt tggctttaat gagttgtaat tcctctgggg
     4201 caactgttcc tttcttcgtt gctcctcttg cccgccaggc gatacttttt acctgatcag
     4261 ctaacgctac gccatcacgt tcctgaccgg ataaaacaac ttcgaacgga tatccttttg
     4321 attgcgttgt acaaggaaca cacagacaca tacctgtttt gttgttgtac atgaaaggac
     4381 tcaggacaac agctggacga tgtccagctt gctcgctacc ttttgtcggg tcaaaatcaa
     4441 cccaaatcag atcgcccata tcgggtacgt atcggcttac cattaccaga cttccttatc
     4501 tttcggctct ccccagtcga tattctcgtg gaggttttcc ggcgtgatgt cgttgaccag
     4561 ttcagcaagc gtaaatacgg gctctttacg cactggctca ataattaatt tgccatccac
     4621 caggtcaatc ttcacttcat catcaatatt cagattgagc gcctgcatta acgtagccgg
     4681 gatccgcacc gccggtgaat ttccccaacg ctttacgcta ctgtggatca taaccctttc
     4741 ctcaaaccgc tatcatatgt agatacagta tatatcaatc tacattgtag atacgagcaa
     4801 atttctgcct aactcccgtg caaccgacgc gcgtcgataa catccggcac ctggttgagt
     4861 ttacccagca cgcggcccag cacctgcagg ttgtaaatct caatggtcat gtcgatggtt
     4921 gccagttgct gttttgtgtc gctacggctg gcaacgccaa gcacgttcac cttctcgttg
     4981 gcgagaatgg tcgtgatatc acgtaacaac ccactacgat cattagccac cacgcggacc
     5041 accagcgaat atccggcgga gtagctctca ccccataccg cgtcaacaat gcgttctggc
     5101 gcatgagagc gcagttccgc cagttgttcg caatcggcgc ggtgtactga aataccgcgc
     5161 ccctgggtaa tgaagccgac aatctcatct ccaggaatcg gctggcagca gcgcgcgatg
     5221 tggtgcatca ggttgccaac accttcgact accacgcgac cgttatcttt actgcggttt
     5281 tgcggcgtgt agcttttttg ctgaagttgc ttcagcgcgg cggcgtcctg ctcttcggca
     5341 ctcggcttat taaattgcga ttgcaggaag ttcaccatct gattgagacg gatatccccg
     5401 ccaccaatcg ccgccagcaa ctcgtcgaca tcattaaagt tgtaacgcgg cagcagatgt
     5461 ttttctgctt ctttcaggct gatccccaga tgttccagct cgtcgtcaag gatttgccgc
     5521 ccagccagaa tgtttttgtc acggtcctgt ttacggaacc aagcgtgaat tttcgaacgc
     5581 ccacggctgg ttgtgacgta accgaggttt gggtttaacc agtcacggct ggggttcggc
     5641 tgtttctggg tgataatttc aatctggtcg cccatctgca gctggtaggt gaacggcaca
     5701 atgcgcccgc caatttttgc cccgatgcag cggtgtccga catcactgtg gatgtggtaa
     5761 gcgaagtcca gcggcgttga tcccgcaggc aaatcaacga catcaccttt cggcgtaaag
     5821 acatacaccc ggtcgtcaaa gacctggctg cgtacttcgt cgagcatttc gccggaatca
     5881 gccatctctt cctgccacgc aatcagttta cgcagccagg caatccggtc ttcatgtccc
     5941 gaacgtgcgc cgccagctgc cgcgccctct ttatatttcc agtgcgcagc aacacccaac
     6001 tctgcatctt catgcatctg tttggtgcgg atttggatct caacggtttt gccacctggc
     6061 cccagaacca cggtatgaat agactgataa ccgtttggtt tcgggttagc gacataatcg
     6121 tcaaactcat ccggcaggtg gcgatagtga gtgtgcacta tccccagtgc ggcatagcaa
     6181 tcctgtaaac gctcggcgac aatacgtacc gcacgcacat caaacagctc atcaaaggcg
     6241 aggttctttt tctgcatttt acgccagatg ctgtagatgt gtttcggacg accatacact
     6301 tccgctttaa cgccttcagc tttcatctca gcgcgcagat gaccaacgaa ctcttcgatg
     6361 tagtgttcgc ggtcgagacg ccgttcatgc agcagtttgg caattcgttt gtattcggtt
     6421 ggatggaggt aacggaagca gtaatcttcc agttcccatt tcagttgtcc gattccgaga
     6481 cggttagcca gcggtgcgta gatgttggta cactcttttg ccgccagtac acgttcatct
     6541 tccggcgcat cttttacttc gcgcagatga gcaatacgct ccgccagttt gatgactacg
     6601 cagcgaaaat catcgaccat cgccaataac atccggcgaa cgttatcgac ctgttcggag
     6661 gaaacagaat cagtgtgcgt cgctttcagc tggcggatcg ccgccatatc acgcacgccg
     6721 tgaataaggt taacaaccga cttaccgacg ctctcacgca gcacatcttc gctgactacg
     6781 ttggcatcag ccagagggaa cagcagcgcc gcccgcagcg tgtcaatgtc catacttaat
     6841 gtcgagagga tctccaccat ctcaacacca cgccacaata acagactggc atccggatgc
     6901 ccctgcgtct gttgcagaca atacgcccaa gtttcggcta agcactcaca cgacttctgg
     6961 ctggtaatac ccagacttgc gatccatttt tccggatcaa attcaccagc cttattgata
     7021 tgtgcacttc ttaccgcaac catcgtcctc tcctttaggg accagacctg ccgaaatcgg
     7081 caaatcgcaa ctatttaacg cgcgagaaaa gtaccatcga ttccagatgt cccgtgtgtg
     7141 ggaacatatc cagcatcgcc agtcgcgcaa tggtatatcc tgcttttaat aacgcttcgc
     7201 tatcccgagc cagcgttgca gggttacagg atacataaac tatacgaata ggttccagtt
     7261 ttataatttg ctgcataaca cctgcggcac ctgctcgcgc cgggtccagc aacactttat
     7321 cgaagccgtt tttcgcccac ggctgctttg tgacatcttc ttcaagattt tcgtgataaa
     7381 acgtcacatt ctgtaagcca ttaagacgcg cattctgctg gcctttttcc accagcgccg
     7441 gaacgccttc tacacccacc acactggcag cttgtgtcgc caatggcagt gtaaagttgc
     7501 ccataccgca gaacaaatcc agtacgcgat cttcaggttg cacatccagc cattccaacg
     7561 cacgcgctac cattttttgg ttcacacccg cattgacctg aataaaatcg cgcgggctaa
     7621 aagttaagcg caacccattt gagtcatacc agggcatctc accagagacg gtttcgagta
     7681 tctcactatc gggggcgaga tacagatcca ggccttcaga atgcgaaaag cgttccagtt
     7741 tttcgcgatc tgccgaactt agcggtgcgg tatggcgcaa aatcatcagc gtgccgctgg
     7801 ttgcctgtac cagttcaaca tgaccaagat ggcgcataac ttgtaagctg cccaagcatg
     7861 ccatgacttt gggcagcaat gcttcaagtt ggggcactaa aatggggcat tgtttaacgt
     7921 cgacaatgtc actggagccc gctttgcgaa accccatctg aagttgctgt gttttcggta
     7981 agtagttcag acttaaacgc gcgcgacggc gatagcccca gggaacatcg gcgatcactt
     8041 cagagacttc gtgtttcatt aatcgggcga gtgccgcact tttgcttcgc tgctgtaaat
     8101 ccacgctggc gtgttgttgc tgacagccgc cgcatacgcc aaaatgagga cagcgtggcg
     8161 tttcgcgtcc cgggctatcg cttaaccggc gtacgacttt agcgcgggca tactgttttt
     8221 tatcttcagt aacagtaact tccgcgtttt cctgcggcaa taatccgggg ataaatagcg
     8281 ttttgccgtt atgtcgcgcc acgccctgac caaaagagtc gaggtcgttg actgaaacgg
     8341 ttatgatctg acgcgtcgtc gtgcgtcgtt ttgcagagta gaattgcgcc attggcgaga
     8401 ctttctcaat ttaacagtgt gaccttaatt gtcccataac ggaactccat gaccaactac
     8461 agcctgcgcg cacgcatgat gattctgatc ctggcaccga ccgtccttat tggtttattg
     8521 ctgagtatct ttttcgttgt gcatcgctat aacgacttgc agcgtcaact ggaagatgcc
     8581 ggagccagca ttattgagcc gcttgcagtt tctactgaat atggcatgag cctgcaaaat
     8641 cgcgaatcta tcggtcagtt aataagcgta ctgcatcgtc gccattccga tattgttcgc
     8701 gcgatttcgg tttatgatga aaataaccga ctctttgtca cctccaattt tcatcttgat
     8761 ccctcatcaa tgcagctcgg cagcaacgtg ccgtttcctc gccagctcac tgtcactcgt
     8821 gacggcgata ttatgatcct ccgcacgccg attatttctg agagttactc ccccgacgaa
     8881 tcgcccagta gcgatgccaa aaatagtcaa aatatgttgg gatatattgc gctggagctg
     8941 gatcttaaat cggttcgctt gcagcaatat aaagagatct ttatttccag cgtgatgatg
     9001 ctgttttgta tcggtattgc gcttattttt ggctggcgct taatgcgcga tgtaaccggt
     9061 ccgattcgca acatggtgaa taccgtcgac cgcatccgtc gcgggcaact cgacagccgg
     9121 gtggaaggat ttatgctcgg cgagctggat atgctgaaaa acggtatcaa ctcgatggca
     9181 atgtcgctgg ctgcttatca cgaagagatg cagcacaata tcgaccaggc gacgtccgat
     9241 ctgcgtgaaa cgctggagca gatggaaatt cagaacgttg agttagatct ggcgaaaaag
     9301 cgcgcccagg aagcggcgcg tattaaatcc gagtttctgg caaatatgtc acacgagctg
     9361 cgtacaccac tgaatggtgt tattggcttt acccgcctga cgctgaaaac agaattaaca
     9421 ccaacgcagc gcgatcacct gaatacgatt gaacgttcgg caaataattt gctggcaatt
     9481 attaatgatg ttctcgactt ctcgaaactg gaagcaggta agctgattct ggaaagtatt
     9541 ccattcccac tacgcagcac gctggatgaa gtcgttactc tgctggcaca ttcttctcac
     9601 gataaagggt tggaactgac gctcaatatt aaaagcgacg tgcctgataa cgtgatcggc
     9661 gacccactgc gattacagca aatcatcact aacctggtgg ggaatgcaat taaattcacc
     9721 gagaatggca acattgatat tctggtagaa aaacgtgcgc tgagtaatac caaagtgcag
     9781 attgaagtgc agattcggga taccggcatt ggtattcctg aacgcgatca atcgcgctta
     9841 ttccaggcct tccgacaggc tgatgccagt atttcccgcc gtcatggtgg caccggtctg
     9901 gggctggtga ttacacaaaa actggttaat gaaatgggcg gcgatatttc gttccatagc
     9961 cagccgaatc gcggttcaac tttctggttc cacattaatc tcgatctgaa cccgaacatt
    10021 attatcgaag ggccatccac ccagtgcctc gccggtaagc gcctggccta tgtcgaacca
    10081 aactccgcag cagcgcaatg cacgctggat attttaagtg aaacgccgct ggaagtggtt
    10141 tatagcccaa cgttctccgc gctgcctccc gcgcattacg acatgatgtt gttaggcatc
    10201 gcggtgacct tccgcgagcc gctaacaatg caacatgagc gattagcgaa agcggtatcg
    10261 atgaccgatt tcctgatgct ggcacttcct tgccatgcac aagtcaatgc agaaaaactc
    10321 aagcaagatg gtatcggcgc gtgtctgctg aaaccattaa cacctacgcg cctgttgcct
    10381 gccctgacgg aattttgtca tcacaaacaa aacacgcttt tgcctgtaac cgatgaaagc
    10441 aagctggcaa tgacagtcat ggcagttgat gacaaccccg ctaacctgaa acttatcggc
    10501 gcattgctgg aagatatggt gcaacatgtg gaactttgcg atagcgggca tcaggcggtt
    10561 gaacgggcga aacagatgcc gttcgatttg atcttaatgg atattcaaat gcctgacatg
    10621 gatggcattc gggcctgcga gctcatccac cagctccccc atcagcaaca aacgccggtt
    10681 atcgcggtaa cggcgcatgc aatggccggg caaaaagaga agctgcttgg cgcagggatg
    10741 agcgattatc tggcgaaacc gattgaagaa gagcgattgc ataatttgtt gttgcgctac
    10801 aagcctggca gcggtatttc ctctcgcgtc gtgacgcccg aagtcaacga aattgtggtg
    10861 aacccgaatg cgaccctcga ctggcaactg gcactacgcc aggcagcagg aaaaaccgat
    10921 ctagcgcgcg atatgctgca aatgttactc gatttcctgc ctgaagttcg caacaaagtt
    10981 gaggagcagc tggttggaga aaacccggaa ggcctggttg atttgattca taaactgcat
    11041 ggcagttgcg gctatagcgg tgtgccgcgt atgaagaatc tctgccaact gatcgaacaa
    11101 cagctacgta gcggtactaa agaagaagat ttggagccgg agctgctgga actgttggac
    11161 gagatggata atgtcgcgcg cgaagccagc aaaattctcg ggtaacggac gtcttgtcga
    11221 gtagcaatcg gctttttatg acgctggttt cagattggtg acaaagtgcc tgtgtttatg
    11281 ccggatgcgg cgttaacgcc ttatccggcc tacaaaacca attaaattca atgaattaaa
    11341 aaatgatgta gacctgatac atagcacaac aggctatttt gcgtttagca tcagtctcaa
    11401 gccggctcca gatagagccg gttttggttt tctgtcttaa cgcaccatgc acgggcgctt
    11461 gttatcgaac gtccagccag gaatcagata ctgcattccc atcgcatcgt cacgcgcgcc
    11521 aagcccgtgt ttctgataca gctcatgggc tttcatcact tgatccatat cgatttctac
    11581 acccagcccc ggtttttccg gcacctgaac cagaccgcct ttgatctcaa acggttcttt
    11641 ggtcaagcgc tgattgcctt cctgccaaat ccagtgcgta tcaatcgcgg tgattttacc
    11701 cggtgcagcg gcggcaacat gggtaaacat cgccagggaa atatcgaagt ggttgtttga
    11761 gtgtgaaccc caggtcaggc caaattcatg gcacatttgc gccacacgta ccgaaccttg
    11821 catagtccag aaatgcggat ccgccagcgg gatatcaacg gattgtaacg agagcgtatg
    11881 gcccatctgc cgccagtcgg tggcgatcat attggttgca gtcggcaggc cagtcgcgcg
    11941 acggaactct gccatcactt cacgcccgga gaaaccttgc tccgcaccat acggatcttc
    12001 tgcataagcc agcgaacctt tcaggtattt accaatttta attgcttcgt tcagcgacca
    12061 ggcaccgtta ggatcgagcg taatacgcgc ctgcgggaag cgttgcgcca gtgccacaat
    12121 agactcggct tcttcttccc cggccagtac accgcctttc agtttgaaat cgttgaagcc
    12181 atatttttca tacgccgctt ccgctaggcg caccaccgca tccggcgtca tcgcttcttc
    12241 atgacgcagg cgataccagt cgcatgagtc atccggctgg ctttgatacg gcagaggtgt
    12301 ggctttgcga ttaccgacga agaacagata accgagcatt tcgacttcgc tacgctgttg
    12361 accatcgccc agcagcgatg ccacgtttac ccccagatgc tgccccagca gatccagcat
    12421 tgccgcttct atcccggtaa ctacatgaat agtggtacgc aaatcaaatg tctgcaaacc
    12481 gcgcccacca gcatcacgat cggcaaaagt attacgcacc agcgtcagaa cgtttttgta
    12541 ttcacccagc gttttaccta ccaccagcgg aatcgcatct tccagcgttt tacggatttt
    12601 ctcgccgccg ggaatttccc ctacgccagt gtgaccggaa ttatctttga taatcacaat
    12661 attacgcgta aagaacggtg cgtgtgcccc actcagattc atcagcatac tgtcatgacc
    12721 cgccaccggg atgacctgca tttcagtaac aacagacgtc gtaaattgag aactcatatt
    12781 tatgtccttt ttcagaatca atgacggccg aaaacgggac gtttacggtc aaaggtccag
    12841 ccggggatca ggtactgcat cggacctgcg tcgttacgcg caccgccagg cagacgttta
    12901 taggcctcat gtgccttttg tacctgttcc cagtccagtt ccacgcccag accgggcgca
    12961 tcaggaacgg caatttttcc gtttttaatc tccagcggat ttttggtcag gcgacaatcg
    13021 ccctcctgcc aaatccagtg ggtatcgata gcggtaggat tacccggtgc cgccgcgccc
    13081 acatgggtaa acatcgccag agagatatcg aaatggttat tagaatggca gccccaggtc
    13141 agcccccagt cgtcgcaaag ctgcgccaca cggactgcac cggaaagcgt ccagaagtgc
    13201 ggatcggcaa gtggaatatc taccgcattg agcatcaccg catgacccat ttcgcgccag
    13261 ttggtggcga tcatgttagt cgcgacgggc aagccggtcg cccgccgaaa ctccgccata
    13321 acttcacgac cggagaaacc ctgttctgcg ccgcatggat cttcggcata ggtaagaaca
    13381 tcattcagcc ctttgcatag agaaatggct tcatcaagta gccaggcacc gttgggatca
    13441 acggtaatcc gcgcatcagg gaagcgtttc ttcaatgcac gaacagtgtc gatttcttgc
    13501 tcgccaggta acaccccgcc cttaagtttg aaatctttaa agccatagcg atcctgtgag
    13561 gcttccgcca gacgcacaac ggcttcgctg ttcatcgctt tctgatggcg caactgatac
    13621 cactcatggt tgcccggcgt attttccaga taaggaagat cggttttggt ccgatcaccg
    13681 atataaaaca gataaccgag gacggtaata gtctcgcgtt gcttgcctgg ccccaacagt
    13741 tcgcagaccg gaacattcag cgccttacct agcaggtcaa gcaaggcggc ttccagcgcc
    13801 gccacggcat taacgcgcaa ttcaaaagtc caggcacctt tgccgaaggt atcaaaatcg
    13861 gctgcctgat tacctttatg cacctgctga accactttat tcaggcgcgc aacttcctgg
    13921 cccagaacca tcggaatagc atcgaccagt gtttgataaa tcacctctcc gcccggcgct
    13981 tcaccaatgc cggtatgccc ggcgttatcg gtgagtacca caatattgcg ggtgaaatat
    14041 gcgttatgtg ccccaccaat attaagcaac atgctatcgt gcccggcgac cggaatgact
    14101 ttcatatcag taataacagg actggattgt gtcgtcatct tattgccccg cgacaggttt
    14161 cagctcgata cgcttgatat cgcccaccag caccaggtag ctcagtaccg cgattaaggc
    14221 atgaacacca acataaatca gcgccccatt aaaggagcca gtcgtgccaa cgatataacc
    14281 aattgcgatt ggcgtgacga tgccagaaat gttaccgaac atgttgaaca ggccaccgga
    14341 aagaccgctg atctcttttg gcgcggtatc tgccattact gcccaaccca gcgccccgat
    14401 gcccttaccg aagaaggcca gcgccataaa gccgatgatc atccactcaa cgttgacgta
    14461 gttgcagaac accatcacca tcgacagcaa catgcccatt acgatcggtg ttttacgcgc
    14521 aatgttcagc gatcccgtgc gacgcatcag ccagtcggaa ataatcccac ccagcacacc
    14581 gccgataaaa ccgcaaaccg ccggaacgga agccacaaag cccgctttca gaatcgacat
    14641 cccacgcgcc tgcaccagat aaaccggaaa ccaggtaatg aaaaagtaag tcagggcgtt
    14701 gatacagtac tgaccgatat aaacgccgat catcatccgt gacccgagca gttgtttgat
    14761 ctgcccccac ttcacgctga acggcacttt aactttggtg ttttgctgat ccatattgat
    14821 cagcgcgcca cccgcggcga tgtactccag ctctttctga tttacccccg gatgttgatt
    14881 tggctcgtgg atgactttca accagataaa gctgatgaca atccccagac cgcccataaa
    14941 gaagaagacg tgtgaccagc ccacttcatg cgtcagccag cccataatcg gcgcgaagat
    15001 caccgttgcg aagtattgag cggagttaaa aatcgacacc gccgttcccc tttcctgcgc
    15061 cggaaaccag gccgcaacaa tgtgactgtt gccggggaaa gatggcgctt cagcaagccc
    15121 gaccaggaag cgcagagtaa acagggcaac gataatgccg aatccactaa agatatcgac
    15181 gaagccttgc agcaaggtaa acatcgacca gataaagatc gaccagaagt agacgcgttt
    15241 tgaaccaaaa cgatccagca accaaccacc agggatctgc ccgataacat aagcccatga
    15301 gaaggcagag aacacatagc ccattcccac gggatcaagg ccgatatctt tggccatttc
    15361 cgaaccggca atagaaagcg tagcgcggtc gccgtagttg aaggatgtga cgataaacaa
    15421 catcaccact atccagtaac gagcattcgt gcgtttttcc acactgctcg cagcctgact
    15481 taaagaactc attgttgcac tcctgaaaat tcgcgttagc cacgctcact ctggactgcg
    15541 acatagccag gaaatcagag gtgacgtagg gtgttttttg ccgtttttat aggtcgttcg
    15601 ccgaatacgg cgcgtgttta tatctggcaa tagcagtata aaaagcgcgc catagcggct
    15661 caccgtgcaa caacacaaca ttaatgcgtt caatgaggcc cgattttggc attagccctg
    15721 gacggtggaa tccacttcac ggaaatgaaa acaagaacaa gaaaggaagg gttaaaacga
    15781 agaaataaag aagagtatga aatggatcgc ttgactccag gcaaacgcca gtaaaaatcc
    15841 gcgctatgaa gcagttttta ctggcatttg cctgaaaaga ttcgattcag caccgctaaa
    15901 acgacattta ccgctcgctg aacatatcag gacaacagcg tgccccactg ttcgacccac
    15961 ggatttgatt ccgtttccgg ttccgggttc tcactggcgt caatcaacag catttcgcca
    16021 acccgctgag cgctctgttc ctgcaacaag gcatcgaact gtttgccgcc attgcagaaa
    16081 tttacataac tgctgtcgcc gagcgcaatc acgccataac gcagattcgg ctggaagccc
    16141 agactatctt tgattccctg aaagagcggc acaatgctat caggaaggtc gccctgcccg
    16201 gtcgtggacg taaccaccag aacatactta tcctgataag gcagccagtc gcttaattca
    16261 ggatcttcaa ataccgttgc tttgtggccc tgtgcggtca gaattgcttc tgcttcttcc
    16321 gccactaaca gcgaatttcc gtacatggtg ccgacaaaaa taccaatttc cgccataccg
    16381 ttttctccct ggattaggaa cttatctctc catcctgacc cgatggcgca ctgaactcaa
    16441 ccctttcatt ttcaggaagc agaccgcgcc agccaaattg tgataacgcc tgcatccagg
    16501 tgtcgtccag gcccgcgtga atagtcagcg gctcgccagt aaaaggatgc gtcaatgaca
    16561 actgactggc atgtagcatt aaccgctgga ggccaaaatg ctcagcaccg ctgcgattct
    16621 ggcgtaaatc gccatgtttg ctatcgccaa taatcggatg acgcaaatgg gcaagatgtc
    16681 ggcgaagctg atgtttgcgt ccggttttcg gctccagttc caccaggccg tagcgcgtgg
    16741 tcgggtaacg tccggtcgct accggcattt ctacggtcgc cagaccgcga taatgcgtca
    16801 ctgctggctg cgggccttta tcttcgcggg caaatttatc agcgattttg tccagttctt
    16861 ccaccagtgg ataatccagc accgcttctt ccatcaacca gccgcgcaca atcgcatggt
    16921 aacgtttctg gatttggtgc tgttcaaact gttgtgccag cagccgtccg gcctcgctgg
    16981 ataatcccat caacaacaca ccagaagtgg gtcggtccag acgatgagca gtaaaaacat
    17041 gctgtcctat ctggtcacgc acggtttgca tgaccactac tttctcgtcg cgatccagcc
    17101 agctacggtg aaccagccag ccggagggtt tatttaccgc aaccagccat tcatcctgat
    17161 agagtatttc cagcattagc tcgcatcatc cgcaaaaaga gcatccagtt tttccaactc
    17221 agccagaata agcgcgcgtt gcggatggtc cgtcgccagc gccatttcat aatagggtgc
    17281 aacagcaaaa gcgcccggta acggctggtt gttatttaac aaatcgtgca ttcgcgggat
    17341 cagcacccac tgcaaccact ccagtggttc catggtgtcc ataaagaacg gttgggtact
    17401 attaaattga tgcggctggg gttcatcgtt tcgccagtgc tgatgttcac gcagtaacgc
    17461 ttcaagcgtc tgcaactgga gacgaacgcg gtcatgagtg gtcataaagc aacttcaata
    17521 agaaaaactg ccgcgaagga tagcaggaat aaaaaaaggg agcactgtat tcacagtgct
    17581 cccggttcgt ttcgcagcat tccagctact tttgttgctc cctgctcatc cttgacaact
    17641 tttcctctgg ccttgcggcc aatcgttcat cctgaactat tgcttcctgc tcacaccacc
    17701 ccgatgtgat acttcatcct gaagtgtccc tggccttcct gacccaccga atcatcctga
    17761 ccggttctca ttctccatcc tggaggtgtc ctttaacgcg tcctgcgtca tcctcttcgc
    17821 ttcatccaga agcctttccc tgaaacacca tcctggtgtg tcctgcagaa gtgtcatcat
    17881 cctgatgttc acttcgttgt ctgactccct gtcgacgaag atagaatcgt ctttttcgcg
    17941 aagtcttaca aggcgcttac agacaatgcc ttaaggaaaa tttcatatga aagttaagaa
    18001 ttaacaatca acattttgat tttaaatgaa aataacgatt cttatctttt taagtattcg
    18061 cttatttcct ggcgatctcc tacagggtct gtaagagatc tctcacaacc agatggggta
    18121 tttggattac agaagaggct taagttgatt aaggaattcc gcaagatttg aggctaaatg
    18181 cgtccgttta cgtgttccca gcgtctcttt gcacacttca ccggagagat tacacaccga
    18241 gatgacctct aactcttctt ccagcgtcgc gataaatagc gtgggcggca gttttagcct
    18301 tttctgagtc accagatgac caatcagatt ttcctgcacc cgacggaagt cgtcttcact
    18361 ccaggtttgc agcaatgtca gtttgatatc gccaaactgc gcgtgcatat ccccggcaaa
    18421 ctgagtcgta taaaaggtat gaattgtggg ttgtatcaca atatcaaaag cgcgttcaac
    18481 cgcgtttaca ttttgttccc cggtaaacgg ctggggttgc cagtatacgg catcttcggt
    18541 agttgaaata atgcacggtg aaggaacacc gtacagttcc tcacttaacg gccagctttt
    18601 atgctcttca tgccacgcat cgtagtagcg tgcagtaaaa tctttcaggg cttgtgcggt
    18661 caaatcgtcc actaatttct ctcttcactt aaaccagata cacttgtcgt ttagtttatc
    18721 tggtttatga cggtgaaaca tgtcttctta tgcaaaccat caggcacttg cgggcctgac
    18781 tcttggaaaa tcaaccgatt accgggatac ctatgacgcc agcctactgc aaggcgttcc
    18841 acgcagcctg aatcgcgacc cgctgggtct gaaagcggat aacctgcctt ttcacggtac
    18901 ggatatctgg acgctgtatg aactttcctg gctgaatgcg aaaggtttgc cgcaggtcgc
    18961 tgtcggtcat gttgaacttg attacaccag cgtaaatctg attgagtcga agagttttaa
    19021 gctctatctc aacagtttta accagacgcg ttttaataac tgggatgagg tgcgccagac
    19081 gctggagcgc gacttaagca cttgcgctca gggtaagatt agcgtggcgt tatatcgtct
    19141 tgatgaactg gaaggccagc cgataggtca ttttaatggc acttgcattg atgaccagga
    19201 tatcactatc gataactatg aattcactac tgactatctg gagaatgcca cctgtggtga
    19261 aaaagtagtg gaagagacgc ttgtcagcca cctgctgaaa tcaaactgcc tgatcaccca
    19321 tcaaccagat tggggttcga tccaaattca gtatcgtgga cgccaaattg acagagaaaa
    19381 actgctgcgt tacctggtct cattccgtca tcacaacgag ttccacgaac agtgcgtgga
    19441 acgcatcttt aatgacctgt tacgcttctg ccagccagaa aaattgagcg tttacgcacg
    19501 ttatacccgt cgtggcggtc tggacattaa cccgtggcgc agtaatagcg attttgtccc
    19561 atcgaccaca agactggttc ggcaataaat tttttctcaa ttttgcgtgc tggattcacg
    19621 cagaaggttg tgaaaggaca tcaggcaggg ctattgtaat caaagggaat gacgatattc
    19681 gtcccataag gagtttttct tgattacaca tattagcccg cttggctcca tggatatgtt
    19741 gtcgcagctg gaagtggata tgcttaaacg caccgccagc agcgacctct atcaactgtt
    19801 tcgcaactgt tcacttgccg tactgaactc cggtagtttg accgataaca gcaaagaatt
    19861 gctgtctcgt tttgaaaatt tcgatattaa cgtcctgcgc cgtgaacgcg gcgtaaagct
    19921 ggaactgatt aatcccccgg aagaggcttt tgtcgatggg cgaattattc gcgcgttgca
    19981 ggccaacttg ttcgcggttc tgcgtgacat tctcttcgtt tacgggcaaa tccataacac
    20041 cgttcgtttt cccaacctga atctcgacaa ctccgtccac atcactaacc tggtcttttc
    20101 catcttgcgt aacgctcgcg cactgcatgt cggtgaagcg ccaaatatgg tggtctgctg
    20161 gggcggacac tcaattaacg aaaacgagta tttgtatgcc cgtcgcgtcg ggaatcagct
    20221 gggcctgcgt gagctgaata tctgcaccgg ctgtggtccg ggagcgatgg aagcgccgat
    20281 gaaaggtgct gcggtcggac acgcacagca gcgttacaaa gacagtcgtt ttattggtat
    20341 gacagagccg tcgattatcg ccgctgaacc gcctaacccg ctggtcaacg aattgatcat
    20401 catgccagat atcgaaaaac gtctggaagc gtttgtccgt atcgctcacg gcatcattat
    20461 cttccctggc ggtgtgggta cggcagaaga gttgctgtat ttgctgggaa ttttaatgaa
    20521 cccggccaac aaagatcagg ttttaccatt gttcctcacc ggcccgaaag agagcgccga
    20581 ctacttccgc gtactggacg agtttgtcgt gcatacgttg ggtgaaaacg cgcgccgcca
    20641 ttaccgcatc atcattgatg acgccgctga agtcgctcgt cagatgaaaa aatcgatgcc
    20701 gctggtgaaa gaaaatcgcc gcgacacagg cgatgcctac agctttaact ggtcaatgcg
    20761 cattgcgcca gatttgcaaa tgccgtttga gccgtctcac gagaatatgg ctaatctgaa
    20821 gctttacccg gatcaacctg ttgaagtgct ggctgccgat ctgcgccgtg cgttctccgg
    20881 tattgtggcg ggtaacgtaa aagaagtcgg tattcgcgcc attgaagagt ttggtcctta
    20941 caaaatcaac ggcgataaag agattatgcg tcgtatggac gacctgctac agggttttgt
    21001 tgcccagcat cgtatgaagt tgccaggctc agcctacatc ccttgctacg aaatctgcac
    21061 gtaatctccc tcccccggtg acgtttgttg ccgggttttc tcgtttttgg tcacataccc
    21121 atcaatttat ttcatttgtt atatgaatgt ttcttaccac cctcacgcga taaatatcat
    21181 tgcagttaat atgtcataca atttatgttg taacgcaaac gtttccctat tttcataaac
    21241 cacattttat cgctatgaaa aagaaattat cgccatgatt aactaaaagt attgattttt
    21301 tcagttcaac ctacatatat tgcgcgcccc ggaagaagtc agatgtcgtt taatgggcaa
    21361 atattgccct taaattctct tttacttttg atttacagag taaagcgttg ggataatcta
    21421 tcttccaagt agattattgt atttgggatc aagatcactg atagatacat aacttgtgtg
    21481 tatctttccg ccctcaaatt attacggcgg taaatgatta agccatcgcc gatagacaga
    21541 tttcattttt acggtcaggc accttcccga gctgaactgg ctaaaagctg aattatttgc
    21601 attcctccag gagaaataga tggaaacgac tcaaaccagc acgattgcgt cgaaagactc
    21661 tcgtagtgcc tggcgcaaga cagacaccat gtggatgctg ggcctttacg gcacggcaat
    21721 cggcgcgggc gtgctgttcc tgccaatcaa cgccggtgtt ggcggtatga tcccgctgat
    21781 catcatggct atccttgcgt tcccgatgac attttttgct caccgcggcc tgactcgctt
    21841 cgtactgtct ggtaaaaacc caggcgaaga catcaccgag gttgtagaag aacactttgg
    21901 tattggcgca ggtaaactga ttaccctgct ctacttcttc gctatctacc cgatcctgct
    21961 ggtttatagc gtggcaatca ccaatacagt tgaaagcttc atgtctcacc agctgggtat
    22021 gacaccaccg ccgcgtgcga ttctgtcgct gatcctgatc gtgggtatga tgaccatcgt
    22081 tcgcttcggt gagcagatga tcgttaaagc aatgagtatt ctggtattcc cgtttgttgg
    22141 cgtactgatg ctgctggctc tgtacctgat cccgcagtgg aacggcgcag cactggaaac
    22201 gctgtctctg gacactgcat ctgcaaccgg aaacggtctg tggatgaccc tgtggctggc
    22261 aattccggtt atggtgttct ctttcaacca ctctccgatc atctcttctt tcgccgttgc
    22321 gaagcgtgaa gagtacggcg atatggcaga acagaaatgc tcgaagatcc tggcattcgc
    22381 acacatcatg atggtgctga ccgtaatgtt cttcgtcttc agctgtgtac tgagcctgac
    22441 tccggcagac ctggctgcgg ctaaagagca gaacatctcg attctgtctt acctggctaa
    22501 ccactttaac gcaccggtta tcgcgtggat ggctccgatt atcgcgatta tcgctatcac
    22561 caaatccttc ctcggccact acctgggcgc acgtgaaggc ttcaacggta tggtgattaa
    22621 atctctgcgt ggtaaaggta agtctatcga aatcaacaag ctgaaccgta tcactgcgct
    22681 gttcatgctg gtaacgacct ggattgttgc caccctgaac ccgagcatcc tgggtatgat
    22741 tgaaaccctg ggcggcccaa tcatcgcgat gatcctgttc ctgatgccga tgtacgcaat
    22801 tcagaaagta ccggcaatgc gtaagtacag cggtcacatc agcaacgtat tcgttgtcgt
    22861 gatgggtctg attgcaatct ccgcaatctt ctactctctg ttcagctaag tcctttcgcg
    22921 ccgctttcgg gcggcgcttc ctccgtttta acacgatgta tttcctatga ttagcgtatt
    22981 cgatattttc aaaatcggca ttggcccttc cagttctcat accgttggac caatgaaagc
    23041 gggtaaacaa tttaccgacg atctgattgc ccgtaatctg cttaaagacg tgacccgcgt
    23101 ggtggttgac gtgtacggct cgctctctct gaccggtaaa ggccaccaca ctgatatcgc
    23161 cattattatg ggcctggcgg gtaacctgcc ggataccgtg gatatcgatt ccatccctgg
    23221 ttttattcag gatgtgaata ctcatggtcg cctgatgctg gcaaacggtc agcatgaagt
    23281 ggagttcccg gttgatcagt gcatgaactt ccatgcagac aacctttctc tgcatgaaaa
    23341 cggtatgcgc attaccgcgc tggcgggcga taaagtcgtt tacagccaga cttactactc
    23401 tattggcggt ggctttatcg ttgatgaaga gcattttggc cagcagaata gcgcaccggt
    23461 tgaagttcct tatccgtaca gttcagcagc cgatctgcaa aaacattgtc aggaaaccgg
    23521 gctgtcactc tctggcctga tgatgaaaaa tgagctggcg ctgcacagca aagaagagct
    23581 ggaacagcac ctggcgaacg tatgggaagt catgcgtggc ggtattgagc gcggtatttc
    23641 caccgaaggc gtgttgcctg gcaaactgcg cgttccacgc cgtgctgcgg cactacgccg
    23701 gatgctggtc agccaggata aaaccaccac tgacccgatg gcggttgttg actggatcaa
    23761 catgtttgca ctggcagtga acgaagagaa cgctgctggc ggacgcgtgg tgactgcgcc
    23821 gactaacggt gcgtgcggga ttatcccggc agtgctggcg tactacgaca agttcatccg
    23881 cgaagtgaac gctaactcac tggctcgtta cctgctggta gccagtgcca ttggttctct
    23941 ttataagatg aacgcgtcaa tttctggtgc tgaagtcggc tgccagggtg aagttggtgt
    24001 ggcgtgctca atggcggcgg ctggtctggc agaactgtta ggtgcaagcc cggcgcaggt
    24061 gtgcatcgcg gcggaaatcg ccatggagca caacctcggt ctgacgtgtg acccggtcgc
    24121 cggacaggtc caggtgccat gcatcgagcg taacgccatt gcggcagtaa aagcggtgaa
    24181 cgccgcacgt atggcgctac gccgtaccag cgagccgcgc gtctgcctcg ataaagttat
    24241 cgaaaccatg tacgaaacag gtaaagatat gaacgccaag taccgcgaaa cctctcgcgg
    24301 cggcctggca atgaagatcg ttgcctgcga ttaatcgctc tccagaggcc tcgttttgcg
    24361 aggcctcttc ccgatttctc atccagccgt agcctgttcc ggcatcgaat gttaccctta
    24421 tcgcctgatc tttaaggggg ttatcgtggc tgttcatttg cttattgtcg atgcactgaa
    24481 tcttattcgt cgcattcatg ccgttcaggg atcgccctgt gtcgaaacct gccagcatgc
    24541 gctcgatcag ctcattatgc acagccagcc aacccacgcg gtcgccgttt ttgatgatga
    24601 aaaccgcagt agcggctggc gtcatcagcg tttaccagat tacaaagcgg gtcgaccgcc
    24661 aatgccggaa gagttgcacg acgagatgcc tgcattacgc gccgcctttg agcaacgcgg
    24721 cgtcccgtgc tggtcaacca gcggcaacga agccgatgac ttagccgcca cgctggcggt
    24781 caaagtgaca caggccgggc atcaggcaac gattgtttcg acagataaag gctactgtca
    24841 gttactttca ccgacattac gtattcgtga ttacttccag aaacgttggc tggatgcgcc
    24901 atttatcgat aaagaatttg gcgttcaacc gcagcagttg cccgattact ggggacttgc
    24961 ggggatcagc agttcaaagg taccgggtgt tgcgggaatc ggaccaaaaa gcgccacgca
    25021 gctgctggtc gagtttcaga gtctggaagg gatatatgag aatctggatg cggttgccga
    25081 aaagtggcgc aaaaaattag aaacccataa agagatggcg tttctgtgcc gcgatattgc
    25141 ccgcttacaa accgatttgc atatcgacgg caatttacag caattgcggt tggtacggta
    25201 acggcgagcc ggatgcaaac gattatcaac acattgaatt taatgcactt atataccgga
    25261 taagacgcgg ctagcgtcgt atccggcatt atcacatcag cgcatttacc aggcggtatg
    25321 gtaaagctct acaatatcct caagcgttgc ttcacgcggg ttgccaccgg tacaaacatc
    25381 atccagtgcc gcctgcgcca gtgccggaat gtcttccttg cgtacaccaa catcacgcaa
    25441 atgtggcgga ataccgacat cacggttgag agcaaacacc gcttcaacag cggcattacg
    25501 cgcctcttcc aggctcatac cttccacttt cacgcccata acgcgcgcga tatcgcggta
    25561 cttctcaccg gtaaagtcag cgttatagtg catgacatgc ggtaacagga tggcgttcgc
    25621 aacaccgtgt ggagtgttgt aaaacgcgcc cagtggatgc gccataccat gcaccaaccc
    25681 taacccaaca ttcgagaagc ccatacccgc aacatactgc ccgagcgcca tttcttctcc
    25741 ggcatcctta tcaccagcaa ccgatcctcg cagcgcccca gcaatgattt caatcgcttt
    25801 aatgtgcagt gcatcggtta gcgcccacgc gccacgggta atatacccct caatagcatg
    25861 agtgagcgca tcgacacccg tcgcagcttt cagcgctgga ggcataccat ccatcatgtc
    25921 agcgtcaata aacgccacct gcgggatatc atgcggatca acgcaaacaa acttgcgccg
    25981 tttctcttcg tcagtgatca cgtagttaat ggtcacttct gccgcagtac ctgctgtggt
    26041 aggaattgcc agaatcggta cactgggttt attggtcggg gaaagccctt ccaggctacg
    26101 cacatcggca aactccggat tgttgctgat aatgccaatc gctttacaag tatcctgtgg
    26161 agaaccacca ccaatagcga tcaggtaatc cgcgccgcta ttctggaata caccgagccc
    26221 ttctttgacg acagtaattg ttgggttggg cactacgccg tcgtaaatcg cccatgccag
    26281 ccctgcagca tccatcttat cggtcacttt cgccaccacg ccgcattgca ccagcgtttt
    26341 atcggtgacg atcagcgcct tctgataacc acggcgtttc acctcatcgg ttaaagcccc
    26401 aacagcaccc cgaccaaacc atgccgtttc gttcagaatc attctgttag ccatcatcct
    26461 tctccttgtt gctttacgaa attactcttc aatccgtaac ccataggttt tgaatttctc
    26521 cagcactacg gcaatctctt catcgctcag cactggcacc gggtccgtaa tcgccagggt
    26581 cgccacgtaa agttgcgcca gcacttcaac ttcatgcgcc agccataacg ctttttccag
    26641 attcacctca caagcgataa gcccatgatg ttgtaacaaa gttgccttac gatttttgag
    26701 agccagcgca acatgttcag aaagttcgcg tgttccaaag gtcgcataag gcgcgcaagg
    26761 aatagaatta ccacctgccg ccgcaatcat gtagtgaata gcggggatcg gtcggttaag
    26821 aatggaaact gctgtgcaat gaacggcatg attgtgaaca accgcgttgg catccggtct
    26881 gctttgatag gccgccatat ggaaacgcca ttcgcttgag gggagctttc cttcctcatg
    26941 tttaccgttg ccatcaataa agacaatatg cgactccgtc agtttttcat atggaatgcc
    27001 tgtaggcgta atcagcatcc catcctgata acgtacactg acgttccccg ctgtcccctg
    27061 gttcagtccc aggcgggtca tttccaggca agtgtcaata atctgacgag caagtttatt
    27121 tcgttccatt agctacctct ctctgattca aaacagggca ataatgttgt tcctttcaca
    27181 ctattgaatt agccgtttaa ttacccacca tcttcttcct gattaacaag aaagaaattc
    27241 acaagcttat attttgtgac ctagctcaac taatcacagt aaataactgc aagttctctt
    27301 tttataaccc cattaaaaat gaccgctctt aaaaatattt atcaaaacgg tcatttttct
    27361 attcacccaa gccaggaatg cccgttttcg gcataaacaa ttaatacagt catctggttt
    27421 gtgtttttta tgatttattt tctgaaacgg gcatgaaatt tcgattatta aagtgatggt
    27481 agtcacataa agtcaccttc cagctaataa gtgtgaccgc cgtcatatta cagagcgttt
    27541 tttatttgaa aatgaatcca tgagttcatt tcagacaggc aaatattcac tgatatgaag
    27601 cccgaactcg ctggttttgc acttttgaaa acataaccga ttacgtgctt aagcttctga
    27661 acctaagagg atgctatggg aaacacatca atacaaacgc agagttaccg tgcggtagat
    27721 aaagatgcag ggcaaagcag aagttacatt attccattcg cgctgctgtg ctcactgttt
    27781 tttctttggg cggtagccaa taaccttaac gacattttat tacctcaatt ccagcaggct
    27841 tttacgctga caaatttcca ggctggcctg atccaatcgg ccttttactt tggttatttc
    27901 attatcccaa ttcctgctgg gatattgatg aaaaaactca gttataaagc aggaattatt
    27961 actgggttat ttttatatgc cctgggcgct gcattattct ggcccgctgc agaaataatg
    28021 aactacacct tatttttagt tggcctattt attattgcag ccggattagg ttgtctggaa
    28081 actgccgcaa acccttttgt tacggtatta gggccggaaa gcagtggtca cttccgctta
    28141 aatcttgcgc aaacatttaa ctcgtttggc gcaattatcg cggttgtctt tgggcaaaga
    28201 cttattttgt ctaacgtgcc acatcaatcg caagacgttc tcgataaaat gtctccagag
    28261 caattgagtg cgtataaaca cagcctggta ttatcggtac agacacctta tatgatcatc
    28321 gtggctatcg tgttactggt cgccctgctg atcatgctga cgaaattccc ggcattgcag
    28381 agtgataatc acagtgacgc caaacaagga tcgttctccg catcgctttc tcgcctagcg
    28441 cgtattcgcc actggcgctg ggcagtatta gcgcaattct gctacgttgg cgcacaaacg
    28501 gcctgctgga gctatttgat tcgctacgct gtagaagaaa ttccaggtat gactgcaggc
    28561 tttgccgcta actatttaac cggaaccatg gtgtgcttct ttattggtcg tttcaccggt
    28621 acctggctca tcagtcgctt cgcaccacac aaagtcctgg ccgcctacgc attaatcgct
    28681 atggcactgt gcctgatctc agccttcgct ggcggtcatg tgggcttaat agccctgact
    28741 ttatgcagcg cctttatgtc gattcagtac ccaacaatct tctcgctggg cattaagaat
    28801 ctcggccagg acaccaaata cggttcgtcc ttcatcgtta tgaccattat tggcggcggt
    28861 attgtcactc cggtcatggg ttttgtcagt gacgcggcgg gcaacatccc cactgctgaa
    28921 ctgatcccca cactctgctt cgcggtcatc tttatctttg cccgtttccg ctctcaaacg
    28981 gcaactaact gaacatattt tccgaataaa gtgaggaatc tgtaatgaaa aaaatcagct
    29041 taccgaaaat tggtatccgc ccggttattg acggtcgtcg catgggtgtt cgtgagtcgc
    29101 ttgaagaaca aacaatgaat atggcgaaag ctacggccgc actgctgacc gagaaactgc
    29161 gccatgcctg cggagctgcc gtcgagtgtg tcatttccga tacctgtatc gcgggtatgg
    29221 ctgaagccgc tgcttgcgaa gaaaaattca gcagctagaa tgtaggcctc accattacgg
    29281 taacgccttg ctggtgctat ggcagtgaaa ccatcgacat ggatccaacc cgcccgaaag
    29341 ccatttgggg ctttaacggc actgaacgtc ccggcgctgt ttacctggca gcggctctgg
    29401 cagctcacag ccagaaaggc atcccggcat tctccattta cggtcatgac gttcaggatg
    29461 ccgatgacac atcgattcct gccgatgttg aagaaaaact gctgcgcttt gcccgcgccg
    29521 gtttggccgt cgccagcatg aaaggtaaaa gctatctgtc gctgggcggc gtttcgatgg
    29581 gtatcgccgg ttctattgtt gatcacaact tctttgaatc ctggctggga atgaaagtcc
    29641 aggcggtgga tatgaccgaa ctgcgtcgcc gtatcgatca gaagatttac gacgaagccg
    29701 aactggaaat ggcactgacc tgggctgata aaaacttccg ctatggcgaa gatgaaaata
    29761 acaaacagta tcaacgcaat gccgagcaaa gccgcgcagt tctgcgcgaa agtttactga
    29821 tggcgatgtg tatccgcgac atgatgcaag gcaacagcaa actggccgat attggtcgcg
    29881 tggaagaatc acttggctac aacgccatcg ctgcgggctt ccaggggcaa cgtcactgga
    29941 ccgatcaata tcccaatggt gacaccgccg aagcgatcct caacagttca tttgactgga
    30001 atggcgtgcg cgaacccttt gtcgtggcga ccgaaaacga cagtcttaac ggcgtggcaa
    30061 tgctaatggg tcaccagctc accggcaccg ctcaggtatt tgccgatgtg cgtacctact
    30121 ggtcaccaga agcaattgag cgtgtaacgg ggcataaact cgatggactg gcagaacacg
    30181 gcatcatcca tttgatcaac tcaggttctg ctgcgctgga cggttcctgt aaacaacgcg
    30241 acagcgaagg taacccgacg atgaagccac actgggaaat ctctcagcaa gaggctgacg
    30301 cttgcctcgc cgctaccgaa tggtgcccgg cgatccacga atacttccgt ggcggcggtt
    30361 actcttcccg cttccttacc gaaggcggcg tcccgttcac catgactcgt gtcaacatca
    30421 tcaaaggcct gggatcggta ctgcaaatcg cggaaggctg gagcgtggaa ttgccgaagg
    30481 atgtgcatga catcctcaac aaacgcacca actcaacctg gccaaccacc tggtttgcac
    30541 cgcgcctcac cggtaaaggg ccgtttacgg atgtgtactc ggtaatggcg aactggggcg
    30601 ctaaccatgg ggttctgacc atcggccacg ttggcgcaga ctttatcact ctcgcctcca
    30661 tgctgcgtat cccggtatgt atgcacaacg ttgaagagac caaagtgtat cgtccttctg
    30721 cctgggctgc gcacggcatg gatattgaag gccaggatta ccgcgcttgc cagaactacg
    30781 gtccgttgta caagcgttaa tacattactc cctgatgtga tgcccggtcg ctccggctac
    30841 cgggcctgaa caagcaagag tggttagccg gataagcaat gttatccggc tatattgcag
    30901 gagcgattat gaaacaagaa gttatcctgg tactcgactg tggcgcgacc aatgtcaggg
    30961 ccatcgcggt taatcggcag ggaaaaattg ttgcccgcgc ctcaacgcct aatgccagcg
    31021 atatcgcgat ggaaaacaac acctggcacc agtggtcttt agacgccatt ttgcaacgct
    31081 ttgctgattg ctgtcggcaa atcaatagtg aactgactga atgccacatc cgcggtatcg
    31141 ccgtcaccac ctttggtgtg gatggcgctc tggtagataa gcaaggcaat ctgctctatc
    31201 cgatcattag ctggaaatgt ccgcgaacag cagcggttat ggacaatatt gaacagttga
    31261 tctccgcaca gcggttgcag gctatttctg gcgtcggagc ctttagtttc aatacgttat
    31321 ataagttggt gtggttgaaa gaaaatcatc cacaactgct ggaacgcgcg cacgcctggc
    31381 tctttatttc gtcgctgatt aaccaccgtt taaccggcga attcactact gatatcacga
    31441 tggccggaac cagccagatg ctggatatcc agcaacgcga tttcagtccg caaattttac
    31501 aagccaccgg tattccacgc cgactcttcc ctcgtctggt ggaagcgggt gaacagattg
    31561 gtacgctaca gaacagcgcc gcagcaatgc tcggcttacc cgttggcata ccggtgattt
    31621 ccgcaggtca cgatacccag ttcgcccttt ttggcgctgg tgccgaccaa aatgaacccg
    31681 tgctctcttc cggtacatgg gaaattttaa tggtccgtag cgcccaggtt gatacttcgc
    31741 tgttaagtca gtacgccgat tctacctgcg aactggatag ccaggcaggg ttgtataacc
    31801 caggtatgca atggctggca tccggcgtgc tggaatgggt gagaaaactg ttctggacgg
    31861 ctgaaacacc ctggcaaatg ttgattgaag aagctcgtct gatcgcacct ggcgcggatg
    31921 gtgtgaaaat gcagtgtgat ttattgtcgt gtcagaacgc tggctggcaa ggagtgacgc
    31981 ttaataccac gcgggggcat ttctatcgcg cggcgctgga agggttaact gcgcaattac
    32041 agcgcaatct acagatgctg gaaaaaatcg ggcactttaa agcctctgaa ttattgttag
    32101 tcggtggagg aagtcgcaac acattgtgga atcagattaa agccaatatg cttgatattc
    32161 cggtaaaagt tctcgacgat gccgaaacga ccgtcgcagg agctgcgctg ttcggttggt
    32221 atggcgtagg ggaatttaac agcccggaag aagcccgtgc acagattcat tatcagtacc
    32281 gttatttcta cccgcaaact gaacctgaat ttatagagga agtgtgaaat gctgaaaaca
    32341 atttcgccgt taatttctcc cgaactattg aaagtgctgg cagagatggg acatggagat
    32401 gaaattattt tttccgatgc tcactttccc gcccattcga tgggaccgca ggtgatccgc
    32461 gctgatggcc tgttggtgag cgacttgctc caagcgatta tcccattatt tgagctggac
    32521 agttatgccc cgccgctggt gatgatggca gcggtagaag gtgacactct cgatcctgaa
    32581 gtagaacgac gttaccgtaa tgcgctttca ctacaagccc cgtgtcctga catcatccgc
    32641 atcaatcgtt ttgcgtttta tgaacgggcg caaaaagcct ttgcgatcgt tatcacaggc
    32701 gaacgagcga agtacgggaa tattctttta aaaaaagggg taacaccgta atctcatatc
    32761 ggtacgcccg catgacgcgg gcggttatcg aatgatgggg tgaaaaatat gaaagcggca
    32821 cgccagcaag cgatagtcga cctgctgctg aaccatacca gcctgaccac ggaagctctc
    32881 tctgaacagc taaaggtcag taaagaaacc attcgtcgcg atctcaatga attacagacg
    32941 cagggtaaaa ttctgcgcaa tcatggacgc gctaaatata tccaccgtca aaatcaagac
    33001 agtggcgatc cctttcacat caggctgaaa agccattatg cgcataaagc agatatcgcg
    33061 cgcgaggcgc tcgcgtggat tgaagaaggg atggtgatag ccttagacgc cagttcaact
    33121 tgctggtatc tggcacgcca gttgcctgac atcaacattc aggtcttcac caatagccat
    33181 ccgatttgcc atgaactcgg taaacgcgaa cgcattcaac tgatcagttc cggcggcaca
    33241 cttgagcgca aatatggctg ttacgtcaat ccctcgctga tttcccaact taaatcgctg
    33301 gaaatcgatc tgtttatttt ttcttgtgaa gggatcgata gcagcggcgc actgtgggac
    33361 tccaatgcga tcaacgctga ttacaaatcg atgctattaa aacgtgccgc gcaatcgttg
    33421 ttattgattg ataaaagtaa atttaatcgt tcaggggaag cccgcatcgg gcatctggat
    33481 gaggtaacgc acattatttc tgatgagcgc caggttgcaa cttctttggt aacagcctga
    33541 cggaaagggt agcaggccga agacgacgcc ccggccttgc ctgttatcgc tcgtcgcgac
    33601 gaccacccac cgcagcccag atgcggcgga cgtgcaccgt cacttcttcg cgatcgtgat
    33661 acaactgccg cgcctgaatc tgagcattta tgccatgttc atcaagctgt gcctgaatat
    33721 acgccagatt gtgtgatact tcttcgtagc gttttttcat cggcagtttg agattgaaaa
    33781 tggtttcacg gcaccagcca ttaaccagcc actgcgccat caatgccgca actttagccg
    33841 gtttttcaac catatcgcat accatccagg agatattgct gcgtgtcgga cggaatttga
    33901 aaccgtcttc ccgcagccac gtcacctgcc cggtatccat cagactttgc gccatcgggc
    33961 cgttgtcgac ggaataaacc cacatgttgc gcttcaccag ttggtaggtc cagccgccag
    34021 ggcaagcgcc taaatccacc gcccacatcc cgttcgccag gcgttcatcc cactcatccg
    34081 caggaataaa cacatgaaat gcctcttcca gtttgagcgt ggaacgactc ggcgcctctg
    34141 ccggaaattt caggcgcgga atgcccatat agaacggcga attattgttg ctgtatgagt
    34201 aaccggtata gcagcagcct ggtgcaataa agaatacatg cacaaccgga cgcttcggcg
    34261 tttcatagtt cgccagcacc ccggcatcgc gcaaggcagc gcgtagcggc acggtgaatt
    34321 tacggcagaa tttcagcaac tctttgcttt cgttggtatc ggcaacttca acacgcagtt
    34381 caccgccctt ctctactacg ccctgcaaca tgtcgacaat gggggtaata cgatcttctg
    34441 gcggcaaatg ctgcaagagt tcccccacca caaaccactg gcgggcaaaa attaatgaac
    34501 tgaacggcag ctcacggatt aacttatcgc catcatcagg ttgataacat tcataaatga
    34561 cataacccgc attctctttc acgcgggcaa aaccgaaaat ttcccgctgg ccggctttat
    34621 cggtaatttc tgcggcgcac tctttttcaa agcccggacg gcacagcaat acaaccttat
    34681 tcatgactta cgcccttgcg ctttaaacgg atagcaccaa ctaacattaa cgcccagccc
    34741 gcgaggaagc tcacgccgcc aaccggtgtg acaaatgccc acaaacgcag atgggacagc
    34801 gccaggcaat aaaggctgcc gctgaacaac accgtgccta acgcgaggaa aacgctactc
    34861 cagtaaaacc agatactgat gcgacgctgc attgccaccg ccagacctaa gatcgccagc
    34921 gtatgaaacg cctggtattc gaggccggtc tggatccagc ccatctcaac ggcccccatg
    34981 gttttactta acacatgcgc gccaaaagcg cccagagcca caaaaatgaa gccgctaatg
    35041 gcggcgaaaa tcagcataaa acggctggtc atggtcgtac cctacgtaaa ttattgttca
    35101 taacgaaagc ggaatttttc ttgttcagca gcggctttcg ccaggatcca ttggcgaaag
    35161 gcggctattt tacccagttc tgcctggctg tcatgacaaa ccagataaaa agcgttttta
    35221 ctgaccagaa catcattaaa cgggcaaaca agacgtccgg cctcgatttc agattgcgcc
    35281 atcacgttat ttgccagcgc cactccctgc ccgtgaatag ccgcttgcag caccatggcg
    35341 ctatgactaa aaattggccc ttgctgaacg ttgatatgat ttaaccccaa ctgtcgggta
    35401 tatgtctgcc agtcacggcg tgacgcatca tgtaataacg tatgtttagc cagatcttcc
    35461 ggggtcttca agggtttttc gcctgtcagc agtagcggcg aacacaccgg caataaatat
    35521 tcggcgtaca gtttttccac ccgtagcccc ggccagttgc cccgaccata aaatatcgcc
    35581 acatcaacat catccgccag cttatcttcc tgacgatcaa ccgcctggat tcgaacgtca
    35641 attcccggat aagctgaatt aaagctggaa agtcgcggaa ccaaccaatg aatggcgaaa
    35701 ctggggagta aactgaccgt caacgccccc ttggcgctac gggcctggag tttacgcgtc
    35761 gcttcggtta attgcgaaaa tatctctttg atatcgagga aatagctttg cccttcctcg
    35821 gtcagcagga gtgaacgatt acggcggcgg aacagtttta gccccaaaaa atcctcaaga
    35881 gacttgattt gatgacttac tgcggcctgg gtcacgaaaa gctcttctgc tgcgcgagtg
    35941 aaacttaaat ggcgtgctgc ggcatcaaaa actcgtaagg catttagcgg tggtaatcgt
    36001 ttagacatgg cttttaaact ttgatgttaa attaatttaa caattagatc acactgtgta
    36061 acctattagt ttttttaatc tgagccatta taaattgtcc gttgagcttc taccagcaaa
    36121 tacctatagt ggcggcactt cctgagccgg aacgaaaagt tttatcggaa tgcgtgttct
    36181 ggtgaacttt tggcttacgg ttgtgatgtt gtgttgttgt gtttgcaatt ggtctgcgat
    36241 tcagaccatg gtagcaaagc tacctttttt cacttcctgt acatttaccc tgtctgtcca
    36301 tagtgattaa tgtagcaccg cctaattgcg gtgctttttt ttaccttgcg atcgcgaatt
    36361 actgatccag ttcgaccatc tctttcacgt cagtacggtt gatctgctgt ttgttgccat
    36421 tagcgtcttt atacgaaatc ataccggtat cgttatcagt ctgtggtttg ccgtcagaga
    36481 cgatggtacg tccgtcattg gtgtgcatca catagttcga accggaacag gcgctcaggg
    36541 caaaagtcag catacaggca gaaataattg cggcagtctt tttcatcatc ttctccttaa
    36601 agcgattaat aaatcttaat agtattgatt aacaggctaa aattaacgcc taacactatt
    36661 cagcatatgt tacttacgcg gcttcgccag gatatccaga taattctgat ggttagcact
    36721 ctccttgtat caaagtgaat tttgcgtcac gatcggtgca tcaagccgag gagtaccatg
    36781 aacgttttta atcccgcgca gtttcgcgcc cagtttcccg cactacagga tgcgggcgtc
    36841 tatctcgata gcgccgcgac cgcgcttaaa cctgaagccg tggttgaagc cactcgacag
    36901 ttttacagcc tgagcgccgg aaacgtccat cgcagccagt ttgccgaagc ccaacgcctg
    36961 accgcgcgtt atgaagctgc acgagagaaa gtggcgcaat tactgaatgc accggatgat
    37021 aaaactatcg tctggacgcg cggcaccact gaatccatca acatggtggc acaatgctat
    37081 gcgcgtccgc gtctgcaacc gggcgatgag attattgtca gcgtggcaga acaccacgct
    37141 aacctcgtcc cctggctgat ggtcgcccaa caaactggag ccaaagtggt gaaattgccg
    37201 cttaatgcgc agcgactgcc ggatgtcgat ttgttgccag aactgattac tccccgtagt
    37261 cggattctgg cgttgggtca gatgtcgaac gttactggcg gttgcccgga tctggcgcga
    37321 gcgattacct ttgctcattc agccgggatg gtggtgatgg ttgatggtgc tcagggggcg
    37381 gtgcatttcc ccgcagatgt tcagcaacta gatatcgatt tctatgcttt ctctggtcac
    37441 aaactgtatg gcccgacggg tatcggcgtg ctgtatggca aatcagaact gctggaagcg
    37501 atgtcgccct ggctgggtgg cggcaaaatg attcacgaag tgagttttga cggcttcacg
    37561 actcaatctg cgccgtggaa actggaagca ggaacgccaa atgtggctgg tgtcatagga
    37621 ttaagcgcgg cgctggaatg gctggcagat tacgatatca accaggcaga aagttggagt
    37681 cgcagcctgg cgacgctggc ggaagatgcg ctggcaaaac gtcctggctt ccgctcattc
    37741 cgctgccagg attccagcct gctggccttt gattttgctg gcgttcatca cagcgatatg
    37801 gtgacgctgc tggcggagta cggtattgcc ctgcgggccg ggcaacattg cgctcagccg
    37861 ctactggcag aattaggcgt aaccggcaca ctgcgcgcct cttttgcgcc atataataca
    37921 aagagtgatg tggatgcgct ggtgaatgcc gttgaccgcg cgctggaatt attggtggat
    37981 taatgacgaa cccgcaattc gccggacatc cgttcggcac aaccgtaacc gcagaaacgt
    38041 tacgcaatac cttcgcaccg ttgtcgcaat gggaagataa atatcgccag ttgatcatgc
    38101 tggggaaaca gcttccggct ttgccagacg agttaaaagc gcaggctaaa gagattgccg
    38161 gatgcgaaaa ccgcgtctgg ctgggatata ccgtgactga aaacggtaaa atgcacttct
    38221 ttggcgatag cgaagggcgc attgtgcgcg gcctgctggc ggtgctgttg actgccgttg
    38281 aggggaaaac cgccgccgaa ttgcaggcac agtcgccgct ggcattgttt gatgagctgg
    38341 gattgcgtgc gcaacttagc gcctcacgca gccaggggtt aaatgcgtta agcgaggcga
    38401 ttatcgctgc gacgaagcag gtttaagcat agaaccgggt cggataagac gttcgcgtct
    38461 catccgacct gattgtttaa ccctgacgcg ccgctttcgc catcatcttc ttcagcgcat
    38521 gagaaaccgc aacaaaccca aaggtggcgg tcaccatcgt tgccgcgcca aatccagatg
    38581 cacaatccat ccgcttcggc ccttctgccg tggctttcat cgcacacaca gtaccgtctg
    38641 actgcgggta caccagcgct tcagtagaaa acacgcaatc gacgccgagc ttacctttac
    38701 tgtttttcac tacgccaaaa tcgcttttca ggcgctcgcg caacttcgcc gccagcggat
    38761 cctgaatcgt tttcgccaga tcggtaacct gaatctgcgt cgggtcaatc tgcccacccg
    38821 cgccaccggt cgtgaccagc gggattttat tgcgccgaca ataagcaatc agcgccgctt
    38881 tgggccgtac actatcaatg gcatcaatca cgtacgaata acccacgctc atatactgcg
    38941 ctacgttatc cggcgtcacg aaatcatcca ccaccgttac acggcactcc gggttaatct
    39001 ggcgaatacg ctccgccatg acttccgctt ttgccagccc gacgttatcg cgcagagcgt
    39061 gaatttgccg attggtattg gtgacgcaca catcatccat atcgataagc gtgattgcgc
    39121 caatccccgt gcgcgccagc gcttccgccg cccaggaacc gacaccaccg ataccaacca
    39181 cacaaatatg cgcgtcagca aacagttgca acgctttttc accatacaga cgcgctgtgc
    39241 caccaaaacg ctgacgccat gcatcactaa ttaccacaga catagcacct cagaattaaa
    39301 aagggtgagg ttatccctca ccctgtcgta tctgtaaaaa cggtatacag aataacacat
    39361 cagccgctaa agacgttacc tgcgcccggg gcggttttca gcacccagac acgtccatag
    39421 tggttgtacc aacctgcgcg gtgtccggct tccggcccga tcccttgata gatatcgaag
    39481 tgttggcctt tgattgcacc accgacatcc agcgccacca tcagacgcag ttcgtactga
    39541 ccattaaatt tgccgttatt atccagcaac ggcacttctg ccagcaaggt agtacctggc
    39601 ggaataatgg aacgatcaga ggcaactgac gcgcgaccaa ccagcggcac cgcacttgcc
    39661 cctttcaccg gtgcaaaaga ttgcggttta aagaagacga aagacgggtt ctgttccagc
    39721 agctcgcgaa cctcggcttc actgtgtgtt tcgccccagt gacgaatcgc ctgcatcgac
    39781 atatcttctt ttttcacttc gccacggtcg atcagcacct taccaatgct gcgataggca
    39841 tgaccgtttt tccctgcata gctgaaaaag ttaagcggac tgccatcacc aaagtcgata
    39901 tacccactac cctgcacatc cataatgaag ttatccatca gggagttact gtaagcgaga
    39961 atatatttat cactcaatgc ccccgcgtag atctccgcac gagacggcag acgaccacgt
    40021 tttggcggca tacggtaaat aggatactgg aactcgccct ggcgggtatg gcgcgcctga
    40081 attaccggcg tgtaatagcc tgtaaactgc acgttaccat agttgtcggc accttccatc
    40141 tgccaggcat caatgccgaa ctggcgcata ttgcgggtat caccgcctgc gcgcagccac
    40201 tcttgcaccg cgttataaac attactctgg ttgccataca gacgcggtga cgaattacgg
    40261 atatggttaa tttgctcggc aaaatcaccg gcgttaatcg gcgcgccaac ggcatctggc
    40321 tggttcacca gagagaaagg ctgggtaaat ttcccgtctt tatattgctg tccgcgatcg
    40381 gttggtttgg aagagcaggc ggcaagcatt gccacaaccg tgcccataag aaggtacttt
    40441 acccaacgtc ctttcattgt tctcttcttc aggttaaaaa ataaggcgca acgaagataa
    40501 caaaccgccg cgtttaatga aatgagcagc ctctccctga cgcaaatttt gcacaaaaaa
    40561 taggctttag tgatttgttt ttgttcaaaa tcatgccaga tccgtgatcg gggtaaaaaa
    40621 aaggttgcat gaaaacgcga gcggagtata gtgcgcatcc acggaggcgg ggtggagcag
    40681 cctggtagct cgtcgggctc ataacccgaa ggtcgtcggt tcaaatccgg cccccgcaac
    40741 caattattga acaccctaac gggtgttttt ttgtttctac tctcccataa aaaaagcgcc
    40801 attcagcgcc tttttatcat ccccttctcg ccagcgttgc cccatcggca aaatacgctt
    40861 taatccccgc aagaatagac tccgcaactt cctgctggaa agtcgccgtt ttcagtttac
    40921 gctcttcctc aacgttactg ataaacgccg tttcgaccag aatggaggga atatctggtg
    40981 cctttagtac ggcaaacccg gcctgttcaa cttgattttt atgcagcttg ttgattttac
    41041 cgagcttatt cagcactgct ttaccaaact taaggctgtc agcaatggtc agcgactgca
    41101 ccatatcgaa catggtgtgg tcgacatagc ggtcaccgct tttgctcacg ccaccaatca
    41161 agtccgaggc gttctgggtt tgtgccagat attttgccgc agtactggtt gcacctttgg
    41221 ttgagagcgc aaacaccgag gaaccgctcg gctggcgact ggtaaaggcg tcggcatgga
    41281 tagagacaaa caggtcagca cgctgtttct gggcttttgc tacgcgcact tgcaacggaa
    41341 tgaagatgtc ttcattgcgc gtcatgtaca ccttcatatt gccctctttc tcgatcagag
    41401 agcgcaaacg gcgagctatt tgcaatacca catctttttc gcgcgttttg tatttcccca
    41461 ccgcaccgga gtcttcgcca ccgtggccag ggtcaagcat aatgacaatc ggacgatcgc
    41521 gccctgcttt acctggttgt ggaccacttt gcgctggcgg cacctgcttt tcgaggtcgc
    41581 ctttgttgta atcctccagc agcgccagca gcgggtcctg catatcctgt gcattggccg
    41641 gatagaggtc catcaccaga cgctctttaa acccggcgac cggcgcaagg gcaaacagct
    41701 gcggttttac gttttgcttt aattcaaaaa ccatccgtac ggtttgcggg tcaaattgcc
    41761 cgacgcgcgc cgacttgatg aacgggtcgt ctgcacgaat ttgtgccgcc atccccttga
    41821 gtaccgagtt caggtttaca tcttcgatat ccaccaccac gcgttcaggg ttactcaacg
    41881 cgaactgctt atatttcagc tgacgatttg attctaccgt cacgcgggtg tagctggacg
    41941 caggccagac gcgcaccgcc acgacctggc taaccgcagc caggctgacc tgacttacgc
    42001 tcaatagcca catagcgccc gcgccttgca gtaaacgacg acggctgatt gcagtgttgg
    42061 atcctgacat gcctctcccg agcaaaagaa atctaagctg tgtaacaagt aaacgactaa
    42121 tttgaccgga aactttagcg aaagacgcat aatctgtcat ctaataaacg gtaaacattc
    42181 tttttatatt cacggcatta ctgataaaaa agtcgctctc gcataaaatt tacacttgca
    42241 ccctgcgaaa aaacagaata aaaatacaat aatttcgaat aatcatgcaa aggggtgtgc
    42301 cgtggtaaag gaacgtaaaa ccgagctggt cgagggattc cgccattcgg ttccctatat
    42361 caatacccac cggggaaaaa cgtttgtcat catgctcggc ggtgaagcca ttgagcatga
    42421 gaatttctcc agtatcgtta atgatatcgg gttgttgcac agcctcggca tccgtctggt
    42481 ggtggtctat ggcgcacgtc cgcagatcga cgcaaatctg gctgcgcatc accacgaacc
    42541 gctgtatcac aagaatatac gtgtgaccga cgccaaaaca ctggaactgg tgaagcaggc
    42601 tgcgggaaca ttgcaactgg atattactgc tcgcctgtcg atgagtctca ataacacgcc
    42661 gctgcagggc gcgcatatca acgtcgtcag tggcaatttt attattgccc agccgctggg
    42721 cgtcgatgac ggcgtggatt actgccatag cgggcgtatc cggcggattg atgaagacgc
    42781 gatccgtcgt caactggaca gcggtgcaat agtgctaatg gggccggtcg ctgtttcagt
    42841 cactggcgag agctttaatc tgacctcgga agagattgcc actcaactgg ccatcaaact
    42901 gaaagctgag aagatgattg gtttttgctc ttcacagggc gtcactaatg acgacggtga
    42961 tattgtctca gaacttttcc ctaacgaagc gcaagcgcgg gtagaagccc aggaagagaa
    43021 aggcgattac aactccggta cggtgcgctt tttgcgtggc gcagtgaaag cctgccgcag
    43081 cggcgtgcgt cgctgtcatt taatcagtta tcaggaagat ggcgcgctgt tgcaagagtt
    43141 gttctcacgc gacggtatcg gtacgcagat tgtgatggaa agcgccgagc agattcgtcg
    43201 cgcaacaatc aacgatattg gcggtattct ggagttgatt cgcccgctgg agcagcaagg
    43261 tattctggtg cgccgttctc gcgagcagct ggagatggaa atcgacaaat tcaccattat
    43321 tcagctcgat aacacgacta ttgcctgcgc cgcgctctat ccgttcccgg aagagaagat
    43381 tggggaaatg gcctgtgtgg cagttcaccc ggattaccgc agttcatcac ggggcgaggt
    43441 tctgctggaa cgcattgccg ctcaggcgaa gcagagcggc ttaagcaaat tgtttgtgct
    43501 gaccacgcgc agtattcact ggttccagga acgtggattt accccggtgg atattgattt
    43561 actgcccgag agcaaaaagc agttgtacaa ctaccagcgt aaatccaaag tgttgatggc
    43621 ggatttaggg taagcgatga aaatcgccgg atgcggcagg cgtaacactc gtgcgtcgca
    43681 tccggcaatt acgtttatcc ccgcgaacta aacaacgccg ccagaccact gcgccgctca
    43741 gtacgagtgg cgattgccgc acttaatata cgctcatcgg catacagcga cagacggcga
    43801 cgcgcgcggg tcaccgcggt gtataccagc tctcgcgtta ctatcggcgt gcgttggctc
    43861 ggtaaaatca acgccgcatg gtcgaactcc gatccctgcg atttatgtac cgtcatcgcc
    43921 cacgtagttt cgtgctctgg caagcgactc ggttgcacag acttaatatt gccgtccggc
    43981 atcgcaaacc agacgcgcgt cccctgcccg cgatccagcg caataccgat atcgccatta
    44041 aacaacccaa gcgcgctgtc attacgggca atcatcaccg gtcgaccttc gtaccaacga
    44101 gagtgcgaat gacgatgaat tttgcgcttc tgttgcataa actgctcaat tcgctcattc
    44161 agtccagcca cgccaaatgg cccttcccgc agggcgcaca aaagttggta ctcattgaac
    44221 gcctgaatga ttaaatccgg ctcggcacgc gcttgcagca gatccagata acgtccataa
    44281 cccgcaagag cttcctcaag catcgcaata taatcttcgc cgctctgtaa aagccgtttt
    44341 tcaatatcag taaaatcctg ctgaaaaacg gttttcactg ccgttttatc accacggttg
    44401 atcgccgcag ctaactgacc aatgccagaa tcgctgccga aacgatagct tttttgcagc
    44461 aggcagagac tgtcgcgcaa agatgccgct tctgtgccgg ttcctgccgg aacgtgagtt
    44521 cccgtcaggc ggcttagctg cccggcacgc tcggcggtaa agccggcatt ggcataagcg
    44581 cagatatcgc ccagcacagc cccagcctca accgaggcca gttgatcacg atcgccgaga
    44641 aagatcactc gcgcatgatc gggcaaggcg tcgatcagtc tcgacatcat aggcagatcg
    44701 atcattgacg cttcatctac caccagcaca tcaagatgca gcgggttacc ggcatgatga
    44761 cgtaaacgct ggctacccgg ctgcgcgccc agcaatcggt gcaaagtgct ggcatcttcc
    44821 ggaatgcgtt tcttttgttc atcggtcagc ggtaactgtc gcaaagcctt gccgagagat
    44881 tcggttaagc gcgcggcagc tttacccgtt ggtgcagcca gacggatacg gcagcgttcg
    44941 ccgtcggcca tttgaattaa cgctgccagc aacttcgcca cggtggtcgt tttaccggtg
    45001 cctgggccgc cggaaatcac cgagatccgc cgcgtcagcg ccactgctgc cgccactttt
    45061 tgccagttaa tttcatcgct tactggaaaa agtttgtcca gggtttgcgc cagtagagct
    45121 tcatcaacct caatggcatg attcacctca ttgaagaagc gcgctaccgt ccgctcgtta
    45181 caccacatgc gattcaaata aagacgatcg ccacagagga tcatcggcgt gggttcatct
    45241 cccctgctga ccgcttgaga agccagcaag gattcttccc aattttgtag ctcaccgatt
    45301 tcactgacac aggtcgccaa cagcggatgc gacgcctcgt tattttccag tcgtgaaagc
    45361 ggcaaacaga cgtgtccctc tccggcatca tgacttaaca gtgccgccgc gagggtgacg
    45421 gcaggatgtt catctcccgc cacggtcagg gcaaattgca catccagcgg gcgtagctgt
    45481 ttgtgctcca cagcttccag taattgcttt tgcaatttca ttacgcctcc tccagggtca
    45541 taccggcaaa catctcatcc atcagggcaa tcaacccggc gttgggtcgg gttgtgtaaa
    45601 tcccttgttg cggatgttct ttatcaacgc cacgcaggaa cagatagata acgccgccaa
    45661 agtgacgatc atagtcgtaa tcagcaatgc gatggcgcag atagcgatgc agcgccaggg
    45721 tataaagctg atattgcaga tcatagcggt gtgcctgcat cgctgctgcc atagcctgtt
    45781 gggtgtaagc cgaactgtct tcacccagcc agttggattt atagtcgagc aggtaataac
    45841 gcccttcgtg gcggaatacc aggtcgataa agccttttaa catgccgcgt acctgcatga
    45901 actccagcgg cgggcagcca gcggatagcg ggtcaaactg gcgaattagt gcatcaagct
    45961 ggctggcgat gagcggttcg ctaatcggca ggtaaaactc catctccacc tgtttatcgc
    46021 ggtcggaaag ctgactcagg ctaacacccg tttcattgag aggtgcctgg aggacagccg
    46081 tgatccactc ggtcaacacc ggttcccact gcgattcaaa gccgccgagt tccagttttt
    46141 cctgtaccca gttcgggtca accggctggg taaaatcgag gtcttcaaac aaactgtgca
    46201 agaatgtccc cggtgacgca ccgcgcggga actgatgcgg tgttaacgtc ggttcttcaa
    46261 cgacgctgac cacgcctgcg gcatcaacat ccagccgtgg catcagatcc tgagcgatac
    46321 cgtgaccacg ctgctgcaag ccggagtagc tggtgacacg ccagttatcg ccgggcaatc
    46381 gttgtagtgt cctcgcattc agttctgcag ttaaagcatc attaacctgc cagggctggt
    46441 tatcaccagt ttgtgccgtt tgccaggcaa tatcatcatc gcataacgct tcaatacagg
    46501 tgcgaagccc tgccgcatct tgcggttccc ctttttgcag caaacgcccg agcgcacttt
    46561 ggtggacgtc ggtgtcacct tttttatcgc cacgacggcg caccagcggt gcaacgccga
    46621 gactgcaatg ccaaaccgaa cgcgtcagcg ccacgtaaag caaacgcaga tcttccgcca
    46681 gacgttcgac ctccgcgagg tcgacgcttt ctggcgcagc attaagatcc aaaactgcct
    46741 caaacgagtg gcgatcgtga taaaacgcct gatcctggac gcggaaattg gtaataaatg
    46801 gcaaccagac cagcggatat tccagccctt tcgatttatg gatcgtgaca atctgcacca
    46861 gatgtttatc actttcgagg cgcatttgtt ggctggaggc attactgtct ggctcgagga
    46921 tatgttgcga taaccagcgt accagcgcat gttcactttc cagctgcgtt ccggcttctt
    46981 gaagcagttc gctgatatgc aagatatcgg taagacgccg ctcaccgcct gccgttgcca
    47041 gcaagttttc tgcaatgtta cgcgccgaca tcagcgcccg cagcatcggc ataacgccac
    47101 gtttgcgcca gatttgccga taaccatcga actcttcgac taccgcatcc cacgcatgtt
    47161 cgtcattatt cagcgtttca atatccagcg cattcagccc catcattgac gttgccagcg
    47221 cactgcgcag ggtgttctca cgttcgggcg tcatcaccgc ctgcaacagc caaagcattt
    47281 cctgcgcttc cagagtttca aaaacactat cgcggttcga aaggtaaacg gaagggattt
    47341 ccagcaacgt taaggcatcg cgcacctggg cggcctcctg gcggctgcgc accagcacgc
    47401 tgatgtccga agcacgcacc ggacgcgcgt cgtcgccgtt catcagcaac gcttcgcccc
    47461 gctgtccggc ttgtagccag tcgcggattt gcgcagcaca tacctgcgcc atggtacttt
    47521 gatagtcgcc aacgccgcag ctttcgcctt ccatcagcca cattttcatc gcaggctgcg
    47581 tttcaccttt aaagacaaaa cgtaacgcct gatttttccc ggctgatttc actggaataa
    47641 acggtatttc gcgaaacatg aacgcgtcat cagtctggct gaaaagcttg ttcacactgt
    47701 tcaccattcc aggtgcggag cgccagttgg tgtctaaagt gtagtgggcg tgaacttcac
    47761 tacgcgcctt catataagtg aagatatccg caccccggaa tgcatatatg gcctgcttcg
    47821 ggtcgccaat cagcaacaat gcggtttctg gctgatggtg ccagatacgg cgaaaaattc
    47881 ggtactgctg ggggtcggta tcctgaaatt catcgatcat tgccaccggg aatcgcgtac
    47941 ggatcgccgc tgccaacacc tcgccgcttt cgctacgcag cgcggaatcg agccgactta
    48001 acatgtcatc aaaacccaat tcgccacggc ggcgtttttc acgcgctact gtttcgcgga
    48061 tctcagccaa tgcgcgggtg atcaccagat cacggatcga caatggttct gcaagcagtt
    48121 gatcgatcgc ctcaaacagt ggatgtcgcg gggtttcccc cccggctttc gtgcgatctt
    48181 ctaagaaacg ctgggagaat ttttccagcg actccggcaa ctgataactg tttgtctctt
    48241 cttctgccca ggcgctgatc ttgtcgatcc atttagcctg attgctacgg ttaaacttgc
    48301 gtcgatcaat accagaagat tcgatcagcg catccagttc acccactgcg tcgcgccact
    48361 gctgttttac cgtatcaata cgcgccacaa tttgcgcgtg acgggaagct agcgtttcat
    48421 catcgggcgg cggtgctttg ataaccggcg cttcgccttg cagataacga ttaatatcgc
    48481 gcagcaacgc ctgcggccct ttccaggttt caaagacgac ctgggctatt tcacgcggca
    48541 gcgggtagca gtggcgacgc cagaaatcgg cgcaggcctg gtagcgtagc agagactcat
    48601 cttcaatcag ctgctgctca aacagcatgc cggattcaaa ggcattcagg ttgagcatgc
    48661 gctggcaaaa gccgtgaata gtaaagactg ccgcttcatc catctgccgt tcggctaaca
    48721 acaaccactg cgcggcttgc gctttatcgt cgacctcttc cagcaggtgt tcgtacaatg
    48781 gattgtcggt ggtttcacgc agacaggcga tgcgcaactc gtggatattg ctacggatac
    48841 gaccgcgcaa ttctgccgtg gcagcctcgg taaaggtgac caccagcagt tcttcaacgg
    48901 tcagcgggcg gggaaaggcg gcggaaccgc ctagtccaag taacaggcgc aaataaagcg
    48961 ccgcaatcgt aaaggttttg cctgtgccgg cagaggcttc aatcaggcgc tcgccctgta
    49021 agggcaagcg caaaggatct agtgtctcgg cgacatcact cattcttttc actcatcagg
    49081 ggcattgttt gctgcaacgc gctgacgttc tcccacactt tccagccttc aggatgcaca
    49141 tattcggctt tcccgttttg gctgccggaa atctgcgaca ggatagccat gccttgcggc
    49201 tcgactaccg cctgatggaa gaaatcggcc agtttttgcg gcgtcagcag tttaatctgg
    49261 gccacgattt tatcacgcga atcgaagcgc atattgccgc gatcgaaatc tttacttaac
    49321 ttcgatgctt cttcgccgag cgtttgcggt gcctgcagca tctgggtaat taccgcctgc
    49381 tggatttgcg caaactcatc tggcttcatc gctcgcaatt ttgcctctgc ggttgggaaa
    49441 aacgccttgt aacgctccca caagaatgaa ggctgtttat cattgctttg caaaaggaag
    49501 cccatgcccc actgacgccc cacgctcatt ggaaacgcaa acacggcata gcccaattgt
    49561 tcttcggtac gcaactgatt gtagaaccac ggctgtacga tctgccccaa cagagagcta
    49621 taggctgagc tggtgtattc atcgtagcca gtcggtacaa ataccgctgc cagtgcggag
    49681 tcggtgctgt taccggcttt ttcaaagatg acggattgtt ttttatcgac cactacatct
    49741 ttgtttcgac accactctga accatcagcg cccaactgtt tttgcacatc gcgtgccagc
    49801 gttgttgcct gggcctcggt catgttgccg ataaccataa actctggtcg agcccctgat
    49861 tttaaggcgt cgcgataagc cagcacctct ttcaacgtaa tggagggcaa aattttacgc
    49921 cgttcatctc gcgagaagta cggcacttgc gagagcatct gcgcgggcat aatcacctgc
    49981 tcaaacgctt tacccttttc tgcggaatcc atcatctggt tataccagga cttcgcctgc
    50041 tcaagctgat cttccgtagc ggtatagcta aagtacccct cgagcaatgc ctggaacagc
    50101 tgcggcagac gctgggtgta accattagca ttaaccataa ggccgttgtt agcgttggtg
    50161 gaaaaactta tgccaccaac cgacgcctgg ttgcttaact gatcaagcgc cagccctgcg
    50221 agataatcat tgagcgcaaa catcacctga ttgcgggcgc tgtccatggc tttcggatta
    50281 cgcaaaatca ggctgacatc agctttgggt tcgctggaaa aataacggct tggcgcatac
    50341 accacgcgca gattcgactc atcaacaatc agctctggat ggtcatattt cttctctgac
    50401 ttaatcagcg agaaatcgtc aggaatatag gggttaagct ccggtaagga gagcgcaata
    50461 ttggcagctt tttgctgcca gtcagcgaaa gtttgttcgc tgattttatc gacctgatac
    50521 ggcgcatcga caaaataagc cgttttattg tgcggctctt tcgggctgat ataccagata
    50581 cgcgcattct gcggcgtcat catcgccaga cgttctttta ctgctttagc atcgtaccga
    50641 tcggcaatat tgactgcatc cagcgtatgc tcaaccggaa cgcgaatcat ggtatctgcc
    50701 agccattcga cgtaatccat atcacgggtg atcgacggat aacggaagtc gatatccagc
    50761 acattcgcca gttcatcgaa gtattgttta tcaatgcctt tttcacgtaa cagattgaga
    50821 tagctaaaaa ttgccgccac aacctgatcg cgattagcca ggcctttatc ggttaaagac
    50881 gcagagatcg ctaatacgcc gctgttgccg ttgacgatag gatcggagtt ggcgctaatg
    50941 ccctcaacta atccctgctt ttgcagccag tcggaaagtg tacctgggct gcgattacca
    51001 atcagatagg taatcaattc atccgtttta ctacggaact tcgctgagtt gttatcgatg
    51061 cgaaactcaa cgcgcagaac tttacgcggc attgccggga cgtaatgaat gataatgccc
    51121 ttttgcgcgt cggtgactac cggcacggtg atttccggtt ttttgctctc tttgttcggc
    51181 acgcgaccaa aggtgtccgc cgccattttt gccaactccg gcagcggttt attgctgtaa
    51241 ataaccgcct tcatcagatt ggcggaatag tacttctcgt ggaaatcttt cagcgcctgc
    51301 tgcaccggat taccaggttt atcgcttaaa gtttcgaggt taccaccaga aaactttgaa
    51361 ccggggtgtg ccgggttaat ggtttctgcg ctgacctgtg ccatgcgcat cccgtcacgc
    51421 gtacgcgcca tggttaattc agcgttcacc gcattacgct cacgttcggc atatttcttg
    51481 tcgagcaaag gttcagcaat agcatcggcc aggcggtcta ccgcaccagg caaggcgtcg
    51541 ttctcaactt ccagatagaa agccgtgcga tacggcgcag tgctggcatt gtgactaccg
    51601 ccgtgcattt tgagatattc ggccagactg tcagcctgcg ggtacttttt cgaccccatc
    51661 agactcatat gttcaaggta atgtgccagc ccctggtacg cctcgggatc ttccagcgac
    51721 ccaacgggca ccaccagcgc cgagagcgat ttaactgcct gcggatcaga aaccagcaag
    51781 accaccatac cgttatccag acgtatagcc tgatactggc ggttatcttt atcactttta
    51841 cggatggttt cctgaatcgg ctgccatccc gtttctgcct gacttaaggg tgcccaaagg
    51901 gcaactaaca acaataatgc tttgaaccag gtgctgcggg gcattctcgg acctcataat
    51961 caacttaatt ttctgtccag attcaacacg ttaacgcaaa tcatctgcca gaatttaatc
    52021 ttgtgctgca cgagtcagcc tatgtttata taaccatcag tccgtgactg gtgcgcatca
    52081 taaagtaagc ggatagattg cgcaattttt atacagcact catgactgat taaagcgaaa
    52141 cagcggtaac aggaaacgtt gcgactgttc aacgatggcc tccattgtct ctggtgttaa
    52201 ttgccgccag agcctttgat accagatatc atcaccttcg ccacgcacca tcatgttacc
    52261 ttcgtaagcc tgaaggaatt tcgtacgggc tttttgcaac gtggaatcgt catccagcat
    52321 ggcatcgttt tgcgcgtcat aacaggtttt tagccacgcg ccgccacttt caggtaacac
    52381 cagcaatggc gcggacatcc cttcacgata cccctcaatc agttgtgaga ggtaatgcaa
    52441 agcctgttcg gctgcaagcg gcggaaaacg ccactcgccg tctttgcgta gaaaaaggcg
    52501 actttcacca ttaccaccgc tggcacagta gacaaggtgt tccagccaaa gttgcattcc
    52561 ctgcgccaca cttaataaag agggacgcca gcgcaacagg ccatccggct gcacctgcgg
    52621 caaccagcca gttatctgca caccgttgca ggcgagatca atttccatac tctgccccgg
    52681 ctggcgacag gcaatgactc tgtcggcaag ctgctgcatc tcctggcact gagtttccca
    52741 gaaaatttca ccaaaagcgc catacggtaa atcccctgcc gcccggaagc gacggaacaa
    52801 gcgttcggca tcatcctgct caaccagtgc attcaataac tgctgattga tttgataacg
    52861 gctaagccct tccagaataa atggctcggt gtcggggatt tcgctgtctt cagtacggaa
    52921 gttcacctgc aaacgcatct ggaagaacgc ccgcaccgga tgtgcccaga atcgttgtag
    52981 cgtttccagc ggcacggttt ccggtaaggt aaacggcagc ggctgaacaa attcagaatg
    53041 tgctttacca gcctggctgg ccgcaggtag ccattcacga gcatagcttt gtcgttcgcc
    53101 tggctgatag ttttgtggat caaacggcat tcgggtatgg aggcaagtaa gatgcgcttt
    53161 tacccttgcc tcgctttcat cacagttgag cgcttcatcg cccggtagat aatgactttg
    53221 cccgatgtag tcgatcagtt cctgcaccag taccgacggg aaacgttcac tgttatcctg
    53281 aatggaacgc ccgatatagc tgatatagag tttttgctgc gcggaaatta acgcttccag
    53341 gaacagatag cggtcgtcat cgcgacggct acggtcgcca cgcttcggtt tctggctcat
    53401 caggtcaaag cccaatggcg caagctgacg tggataaacg ccgtcgttca ttcccagcag
    53461 gcaaaccact ttgaacggaa ttgaacgcat tggcatcaga gtacaaatgt taaccggtcc
    53521 ggcgagaaaa cgctggctga tacgttcttg atccagacgc tgtgccagtt catcacgcaa
    53581 tagtgacagc ggcaccgcgt cgccatactg cgcacctaaa ccttcggcga taatcgcctg
    53641 ccattgttgt tcgatcagcg tcatcgccgc ttcggtttcc gcatccggca ggaagaaggc
    53701 gctgagcata tcgcgacaaa ccggcaacca ctcttctagc ggacgctcct gtgccagccc
    53761 gcgacgccag atgtttagct gcattagcag tgaagccaga tgccccacca gttctgcaat
    53821 taagccgctc gattcatcat aaggtagaac cgattgccac tcgccctgcg cgctctccat
    53881 cgcgtagccc agcaacatac gcgtcaggcc aaatcgccag gtgtgttgtc cggtggcggg
    53941 gagttccagc tcgcgaacgt tgtcgtcatc tatcccccaa cgaatgccgg attcgttgac
    54001 ccactggcgt aaataacgca gcccttcttc ggtgatgtca aaccgcgccg ccagcaccgg
    54061 cacatccagt aatgccagca cgtcttccga cacaaagcgg ctgtctggca acgataacag
    54121 gctgataaac gcttccagta ccggatgcga ctgccgcgcc cgacggtcgg aaatggcgta
    54181 aggcaggtaa cgatccgcag gtgcgctacc aaacacagcc cgaataaacg gactgtagct
    54241 gtcgatatca gccaccatca cgatgatgtc gcgcggagta agtgtcgggt cttcttccag
    54301 catcgccagc aggcgatcgt gcaaaacttc aacttcacgc tgcgggctat ggcaaacgtg
    54361 gaaggtgata ctgctatcca gtggatcaag cgggcgtttg ttatcgctac gggaaaactc
    54421 ttcgatgttc acaccagcaa cggcacggtt ttccagttcc agaatgtcag actgaatgtt
    54481 atgcagcagg ttatctggcg tgacatcgac aaaggcgtcc agctcctggc tgctctccag
    54541 gtcagaaagg agataaatgt agtcgcgccc aagcttaccc catgaagcca gcagcgggtt
    54601 gccgacatcc tgttcaccat cgctgttaaa gagctgcccg gcattttcgc tatcacgaaa
    54661 tagcggcaat tcgcgatctt caaaactgtg tcgacgctga cgggtcagca gtttcgccag
    54721 ataagcagga tctttaatat cgccccagta ataacggcag gggttggtaa acaggagatg
    54781 gatttcaata tgtttaccca gcgcctgtag cgcctggaga taaacaggcg gtaacgcgga
    54841 aataccgcat ataaagacgc gcgaaggtaa ccccggcggg caggtcgtcg cggactccag
    54901 cgtttcgata aagcgctgat agagattggc gcggtgccag cgcggttgcc cgagttgatg
    54961 ggtatattcc accagcgcct tccacaacgg ggcttgccag gcctgtgctt ctcccagccc
    55021 ttcaaccaaa tgtcctgttt cccactgtgc cagccagtcc ggacgataga ccagatactg
    55081 gtcaaacagg tccgccgctt ttgaggaaag ctggaacagt tttcgcttgt cgctatcgtc
    55141 agtcagataa tgccgcaaca gggtaaagtc ttcgcgctcc agcaattgcg gcagcagagt
    55201 catcagtttc cagctcatgc tctgtttgtt aaaggcgctc tctttgggga tttccggtaa
    55261 cacccggacg aacatatccc agataaagct cgctggcagc ggaaaatcaa tgtttgccgc
    55321 aatactaaac ttttgcgaca gggtcatttg cagccactgc gccataccgg tactttgcac
    55381 cagaatcatc tctggttcga aaggatcgtc cagccgttcg cgttcgacaa taaactccat
    55441 caacgcttcc agcacgtcca gacgattgga atggtagacc cttaacatag cggctcctga
    55501 ctactgacga ttcgggcaat gcaggcgggt catctcgcct tctctgcccc cgggtgaaac
    55561 tagcgtaacg ctgatgctga cacatcccgc ctgcgatgtc tgcattcggt tgacctgcca
    55621 gttggcaggt ggcgaaatcg cgcgcagttg cgtttgctgc caaccatgcc gccagagctg
    55681 ttggtactgg tttctgctgg cgagactgtt cattaatgtt cgctggatac ccgataacgc
    55741 agtgacaatc atcaccatca gcaccatcgc aaccattacc tccggcaggc taaagccttg
    55801 ttgattcttc agggaagctg acataacgcc ctctctttca gcggacaaaa atcgctccag
    55861 ccgcgtggcg aaaaaacaat gtttccatca atgacttcgc ctgttcgcca caacgaaacg
    55921 ccttcataac cggcaaccaa taaggcttca ttatctgcca gtaaacgcaa acaaacccgg
    55981 gcatcggttc cagcgtactg cgagcactga actgcgggct gcgtctgcca ggagtgcatt
    56041 ttcccccacg ccagcgccga ctgaacgatg gcctggcggc gcaatgactg actttctatg
    56101 ctcacgcgag aggcaaaact gcgatcctgc tgactcattc cttgcaatag caagctaccc
    56161 aaaaccagca gcatcaggac cagagccagt gacgaaacac ctttttcgcg gttcacaggt
    56221 tgaatcctgt cacgctatag ctggcatcca ccacggtttg cggttcagac ttactggcag
    56281 cacgcatatt aaccgtcaac accggcgaga agccgctgac atcctgacgt acgacctgaa
    56341 aagtgtcgat aatgatggca tccggattag tgactttatc ccagccctta ccttcacagg
    56401 atgtcgcacc gcgtagcgtt tccagcacat gctccttcag acgaaatcca atctggtcag
    56461 actcttttac cggttcgcga tcccagatac cgttactgtt cgcatcccac tgcacaatga
    56521 tacagtcacc ctgtccgaca atttccagcc cttcgccggt acagctgcca tgacaataac
    56581 ccgccctctg gagatgcttc gcgacggtaa atacccgcag ccagatttca tcttccagcg
    56641 ccagcttacg ggtgctcgtt aaactttcac gctgtaacgc aggcagaaag cgtgcagccc
    56701 ccagcaacaa tacgctactg atcgccatag caatcaacac ttccagcaga gaaaaacctt
    56761 gctcttttac aggcatcctt ctgtttctcc ttgctgacaa agccggagtc ttccccacgg
    56821 cgaaaccacc agccaccact cgcccgttga gtttttgaag cgaatatgcc cggcccatgc
    56881 ggtattgcgc aggccaaaga aagcaagcga aggtgtcagg tcgctcattt cgacttcggg
    56941 ccagcgtggc acaaagacca atggtgaact gccatgacag gtattggccc cagcagcgga
    57001 actcacaagg caccataacg tcccctccct gataacgctg atactgtggt cgcggttatg
    57061 ccagttggca tcttcacgta aatagagcaa atagtcccgc gcctggctgg cggtttgcca
    57121 tagccgttgc gactgctgcc agtattgcca gccatagagt ccacttgcgc ttagcatgac
    57181 caaaatcagc atcgcgacca gcgtttcaat cagcgtataa ccacgttgtg ttttcatgcc
    57241 ggcagtatgg agcgaggaga aaaaaagacg agggccagtt tctatttctt cggcgcatct
    57301 tccggactat ttacgccgtt gcaggacgtt gcaaaatttc gggaaggcgt ctcgaagaat
    57361 ttaacggagg gtaaaaaaaa ccgacgcaca ctggcgtcgg ctctggcagg atgtttcgta
    57421 attagatagc caccggcgct ttaatgcccg gatgtggatc gtagccttca atctcaaagt
    57481 cttcgaaacg gtagtcgaag atggattcgg gtttacgttt gataatcaac ttcggcagcg
    57541 gacgcggttc gcggcttaat tgcagatgag tttgatccat atgattgctg tacaggtgcg
    57601 tatcgccacc ggtccagaca aaatcaccca cttccagatc gcactgctgc gccatcatat
    57661 gcaccagtaa cgcgtagctg gcaatgttga acggcaggcc gaggaagacg tcacaggagc
    57721 gctgataaag ctggcaagag agtttgccgt ctgccacata gaactggaag aatgcatggc
    57781 acggtgccag cgccatttta tccagttcgc ctacgttcca cgctgaaaca ataatgcggc
    57841 gggaatccgg gtcgtttttc agctggttca gtaccgtagt gatctggtca atatgacgac
    57901 catctggcgt tggccaggcg cgccactgtt taccatacac tggtccgagg tcgccgtttt
    57961 catcggccca ttcgtcccag atggtgacat tgttttcgtg tagataagca atgttagtgt
    58021 cgccctgcag aaaccacagc agttcgtgga tgatggaacg caggtggcaa cgtttagttg
    58081 tcaccagcgg gaatccatcc tgcaggttaa aacgcatctg atgaccaaaa atggaaagcg
    58141 ttccggttcc ggtacggtcg tttttctgtg tgccttcgtc gagcactttt tgcatcagtt
    58201 ctaaatactg tttcatggtt cctcaggaaa cgtgttgctg tgggctgcga cgatatgccc
    58261 agaccatcat gatcacaccc gcgacaatca tcgggatgga aagaatttgc cccatgctga
    58321 tgtactgcac ccaggcaccg gtaaactgcg cgtcgggctg gcggaaaaac tcaacaatga
    58381 tgcgaaacgc gccgtagcca atcaggaaca agccagagac agctcccatt gggcgcggtt
    58441 tacgaatata caggttgagg ataataaaca gcaccacacc ttccagcagc agctcgtaaa
    58501 gctgtgatgg gtggcgcggc agcacaccgt aagtgtcgaa aatggattgc cactgcgggt
    58561 tggtttgcag cagcaaaata tcttctgtac gggagcctgg gaacagcatg gcaaacggga
    58621 agttcgggtc aacgcggccc cacaattcac cgttaataaa gttgcccaga cgcccggcac
    58681 caagaccaaa cggaatgagt ggtgcgataa aatcagaaac ctggaagaag gaacgtttag
    58741 tacggcgggc aaagataatc atcaccacga taacgccaat caggccgccg tggaaagaca
    58801 tgccgccgtc ccagacacgg aacagataca gcggatcggc cataaactgc gggaaattgt
    58861 agaacagaac ataaccaata cgtcccccga ggaagacgcc gaggaagccc gcatagagta
    58921 agttttcaac ttcatttttg gtccagccgc tgcccggacg attcgcccgt cgtgttgcca
    58981 gccacattgc aaaaatgaaa cccaccagat acatcaggcc gtaccagtga agcgccacgg
    59041 gtcctattga gaaaatgacc ggatcaaact ccggaaaatg cagatagcta ctggtcatct
    59101 gtcaccacaa gttcttgtta tttcgctgaa agagaacagc gattgaaatg cgcgccgcag
    59161 gtttcaggcg ctccaaaggt gcgaataata gcacaagggg acctggctgg ttgccggata
    59221 ccgttaaaag atatgtatat gatccgcgct ataaccctcc gcgaatcagc ccgcccatgc
    59281 cgcgacgctc cataaaggct gcaacctgat ggcgaacttc ggtcgccagt tgcgcttcca
    59341 gactacgctg cgcaagattt tctgcttcgg cataatcaat gcgccgcagc aggtatttta
    59401 cccgcgctac agaacgtccg ttcatagaca gatggcgata cccaagccca atgaggattg
    59461 ccacgcacat gggatcgccc gccatttcac cgcacaaacg gagatcgatt ccatgtattt
    59521 ccgcttcccg ggcgatcatc gccagagctc gtaacattgc aggatgaaga ctgtcataaa
    59581 tgttcgccac ccgggtattg ttgcgatcaa cggccagaat gtattgagtc agatcgttgg
    59641 tgccaacaga gatgaaatcg acccgctttg ccagatgcgg cagcataaat accattgacg
    59701 gcacttccag catgatgccg atacgtggtt tgggaatttc gtaaccgatc atctcctcga
    59761 cttcacgtcc ggcacgttca atcaggcggc gtgcttcatc aacttcatcg aggcttgtga
    59821 ccatcggcaa cagaatattc aggttgcccg tagcggcatt agcacgcagc atcgcccgca
    59881 cctggatcaa gaagatctcc ggctgatcga gcgtaatgcg aatcccacgc caacccaggc
    59941 atggattctc ttcgctgatc ggcatgtaag gcagctgctt atctgctccg acatccagcg
    60001 tacgcaaggt gacgggttta tcattgaaca tttgcagcat cccctgatac tgcgccacct
    60061 gttcttcttc cgacgggaaa ccactttgca gcatgaatgg gatttcagtg cgataaagac
    60121 cgatgccatc aatacggctg cccagttttt cttcatgttc cgggcttaaa ccagcattga
    60181 gcatgacttt tatacgctca ccgcttttta actgcgcggg taaattgacg tcatcttccg
    60241 ccagacggct aagctcaatc tcttcactaa ttagccgctg atattcttgc agcagtaccg
    60301 gctccggatc gaccagcaat tcaccgcgat agccatcaac gatcagcgtc ctacgatgca
    60361 gcaccgaagg ctgaatatcc gcgcccatca cggtagggat ccccagcgca cgtaccatga
    60421 tcgcagcatg ggagttggct gcgccatctc gcacgacaac accgactaag cgatcctggg
    60481 gcagctcagc aagagttgtc gctgacagtt catctgccac cagaatgaaa cgttccggcc
    60541 aggcgttcgg cccttgatta gcgtcatcaa gatgaaacag caatcgctga cccagcgcac
    60601 gtaaatcgcc agcccgctct ttgagatagt tatcgcttag cgcggcaaac tgttcggcaa
    60661 atttttcaat gaccgttttt accgcccact ctgccaccga gcctttatca acctcggcaa
    60721 acaattcgcg acgcagccgg gtgtccgaaa gtaggtgcga gtaaagatcg aaaatagccg
    60781 ccgtttcttt ttgtgctccg gcggcaaagc gtttgctgta gcggcgaaac tcgtttgccg
    60841 cttcttccag cgccccggtc agtcgttcgc gttccagagc cggatccagc gttgatgcct
    60901 gatacacctg ttccattaaa ggtaacgtgg catcctgcca gccttcggca atcgccacac
    60961 caggtgctgc cggtaatgcg cggattcgcg tctggcgata ttgcccaaac aaggcagtca
    61021 actgcgactg agaaagaata gctgccatct gggtggcaag cgtcaccagg aaggattctt
    61081 cactttcgtc atactggcgc aactctcgtt gctgtaccac cagtacacca agcaactggc
    61141 gacgttgaat aattggtacg cctaaaaacg cgcggaaacg ttcttctttt acggagggga
    61201 tgtatttgaa gctggggtgc ttttgcgcat ctgcaaggtt tatcggttcc gccagcctgc
    61261 caaccaggcc gacgatccct tcatcaaacg cgagcgttac agtgcgaccg cgtggttttt
    61321 tcagcccccg ggtcgccatc aggtagtaac aacgtcgatc atgatcggcc aggtagaccg
    61381 aacagacctc ggtatccatc gcaagacaga tgtcggtaac cagaatattt aacgcctcat
    61441 tcaggcgtgg tgcgctggct accttttcga ctatttcgcg caggcgagtg agcataattg
    61501 gcgtgactta acctctttta cgtcgataag cagatgtgtt ttgtggtttt ggcgtatttt
    61561 cctgcagtga catcaccaca ctcgcgaact ctttcattac cctacggtag acatcacgtt
    61621 taaatgacac cacctgtctg accggatacc agtaacttac ccatcgccag ccgtcaaact
    61681 ctggtgtact gctggtttgc atattgattt ctgcatcgcc gctcaccagc tgcaagagaa
    61741 accatttttg tttttggccg atacaaaccg gcttcgtgtc ccaacgcacc aaacgtttcg
    61801 gtaatttgta gcgcaaccag ttacgcgttg aagcaaggat tcgaacgtct ttgcggctta
    61861 atcctacttc ttcaaacaat tcacggtaca tcgcctgctc tgcggattct ccggggttga
    61921 ttccgccttg cggaaattgc caggagtgct gaccaaatcg ccgggcccac attacctgcc
    61981 cctggcgatt acaaatcacg atacctacgt ttgggcggta gccatcgtca tcaatcaccg
    62041 gactacctca aaataaagct ttatatacga atgattgttt catactccaa gaagacggta
    62101 aaccactctc tgcagggcat tacacactaa taacaattga ataactcaca gttatgtgca
    62161 gagttataaa cagaggaagg ggtggatagc cgtttttatt caccttttct gtggatagag
    62221 ttgtgaagaa ctacggaatt actacgggaa aacccggagc attctgaata agcccgatat
    62281 agacatttta aaatattcat tattagtcaa tgcattacat gtaatttgag taatgaaaaa
    62341 gagtaattcg tgacccaggt cacacctctc atttacgggt tggcgaaaga tcaaccaatg
    62401 ccgtatttat ccacagaatg tgccactaag ttaagcactg aaccactaaa aactggagtt
    62461 tcgtcgcacg tcaaggctgt aaatggaaac agtagtggag gtttttcaca gttatcccag
    62521 ctttctgtgg ataacatggt gtaagatcct gtttattttc agtgaccaga tttggaaaac
    62581 ctattgcagt gttgcgcaac tcgattaccg gcagcctaaa aagcaatata aatcagagaa
    62641 ttgaacaaca catgtggaaa aagttacact gcgaatattc ggcacataat tgctgtttgt
    62701 tttttaacca aggtatcatg acatgtccca acctcgccca ctcctctctc ctcccgaaac
    62761 tgaagaacag ttgttagcgc aagcacagca actttctggt tatacattgg gagaactggc
    62821 ggcacttgcc gggctggtta cgccagagaa tttaaaacgc gataagggct ggattggcgt
    62881 gttactggag atctggctag gtgccagcgc agggagtaaa cctgagcaag attttgctgc
    62941 tctgggcgtg gaacttaaaa ctatccctgt ggatagtctt ggtcgtccgc tggaaacaac
    63001 attcgtttgt gttgccccgt taacgggtaa tagcggggtg acctgggaaa ccagccatgt
    63061 gcgccacaag ctcaaacgcg tactgtggat accggttgaa ggtgagcgca gcatcccgct
    63121 ggcgcagcgt cgcgtaggat caccgttgct gtggagcccg aatgaagagg aagaccgaca
    63181 gctacgcgaa gactgggaag aattaatgga tatgattgtt ctcggtcagg ttgagcggat
    63241 caccgctcgt cacggggagt atttacagat acgaccgaaa gcagcgaatg cgaaagcgct
    63301 taccgaagcc attggtgccc ggggcgaacg gattctgacg ctgccacgcg gcttttattt
    63361 gaagaagaat ttcaccagtg cactactggc ccgtcatttt ctgatccagt agccatcgct
    63421 ttgacctgcc gctttccggg catataatta ccgcttcatt tttttggcag ggctttttag
    63481 atgttatttg catggataac cgatcctaac gcctggcttg cgctcggtac gctgacgctg
    63541 ctggagatcg ttcttgggat cgacaatatt attttccttt ctctggtggt ggcaaagctc
    63601 cccacagcac aacgtgctca tgcgcgccgt ctggggttgg cgggagccat ggttatgcgt
    63661 ctggcgctgc tggcatcaat cgcctgggtt acgcgcctga caaatccgct ttttacaata
    63721 ttcagtcagg aaatttccgc ccgtgatttg attctgcttc tgggtggctt gttccttatc
    63781 tggaaagcca gcaaggaaat ccacgaatcc attgaaggtg aagaagaagg gctgaaaaca
    63841 cgcgtttcat cattcctcgg cgctatcgtg cagattatgc tgctggatat tatcttcagc
    63901 ctcgactcgg tgattaccgc tgtgggtctg tcagatcacc tgtttattat gatggccgcc
    63961 gtggtgattg ccgtaggcgt gatgatgttc gccgcgcgct cgattggtga ttttgtcgaa
    64021 cgccatcctt cggtaaaaat gctggcgctc tctttcctga ttctggtcgg ctttaccctg
    64081 attctggaaa gtttcgacat ccacgtaccg aaaggttaca tctacttcgc gatgttcttc
    64141 tctattgcgg ttgaaagcct caacctgatt cgcaacaaaa agaacccgct ctgatactcc
    64201 gtacgctctc ctgcgggagg gcgtactcac tcctgttacc ctcacagttt aagattttcc
    64261 tgctttcaag atatataacg tcggtttata aacagactat tatcataggt gggccaaatg
    64321 aggataaccg gatgaaaaaa tgggcagtaa taatttctgc agtcggactg gcgtttgctg
    64381 tttccgggtg ttccagtgat tacgtcatgg cgaccaaaga tggccgtatg attttgaccg
    64441 atggaaaacc tgaaattgat gatgataccg ggctggtgag ttatcacgat cagcaaggta
    64501 acgcgatgca aattaaccgt gatgatgttt cgcaaattat tgaacgttaa caaataaggt
    64561 cagcatccgg ctgtaatgcc gatcagttaa g
//
LOCUS       DADMKF010000004        63003 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.004, whole genome shotgun sequence.
ACCESSION   DADMKF010000004 DADMKF010000000
VERSION     DADMKF010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 63003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 63003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63003
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.004"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(1..413)
                     /locus_tag="JNC24_000219"
                     /pseudo
     CDS             complement(1..413)
                     /locus_tag="JNC24_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417214.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
     gene            complement(519..3080)
                     /gene="mutS"
                     /locus_tag="JNC24_000220"
     CDS             complement(519..3080)
                     /gene="mutS"
                     /locus_tag="JNC24_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAY9157079.1"
                     /translation="MSAIENFDAHTPMMQQYLRLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            3156..3290
                     /locus_tag="JNC24_000221"
     CDS             3156..3290
                     /locus_tag="JNC24_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9157080.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIASVE
                     "
     gene            3367..3720
                     /locus_tag="JNC24_000222"
     CDS             3367..3720
                     /locus_tag="JNC24_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HAY9157081.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKPRE"
     gene            complement(3757..5834)
                     /gene="flhA"
                     /locus_tag="JNC24_000223"
                     /pseudo
     CDS             complement(3757..5834)
                     /gene="flhA"
                     /locus_tag="JNC24_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
     gene            complement(5908..6918)
                     /gene="hypE"
                     /locus_tag="JNC24_000224"
     CDS             complement(5908..6918)
                     /gene="hypE"
                     /locus_tag="JNC24_000224"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HAY9157082.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARAAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISEVALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            complement(6915..8036)
                     /gene="hypD"
                     /locus_tag="JNC24_000225"
     CDS             complement(6915..8036)
                     /gene="hypD"
                     /locus_tag="JNC24_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HAY9157083.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQENEA"
     gene            complement(8036..8308)
                     /gene="hypC"
                     /locus_tag="JNC24_000226"
     CDS             complement(8036..8308)
                     /gene="hypC"
                     /locus_tag="JNC24_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HAY9157084.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            complement(8299..9171)
                     /gene="hypB"
                     /locus_tag="JNC24_000227"
     CDS             complement(8299..9171)
                     /gene="hypB"
                     /locus_tag="JNC24_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HAY9157085.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPKMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHAPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            complement(9175..9525)
                     /gene="hypA"
                     /locus_tag="JNC24_000228"
     CDS             complement(9175..9525)
                     /gene="hypA"
                     /locus_tag="JNC24_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HAY9157086.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            9737..10198
                     /gene="hycA"
                     /locus_tag="JNC24_000229"
     CDS             9737..10198
                     /gene="hycA"
                     /locus_tag="JNC24_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HAY9157087.1"
                     /translation="MTIWEISEKADYIAQRHCRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            10323..10934
                     /locus_tag="JNC24_000230"
     CDS             10323..10934
                     /locus_tag="JNC24_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HAY9157088.1"
                     /translation="MNRFVIVDSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRIDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARISTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            10931..12757
                     /gene="hycC"
                     /locus_tag="JNC24_000231"
     CDS             10931..12757
                     /gene="hycC"
                     /locus_tag="JNC24_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HAY9157089.1"
                     /translation="MSAISLINSGVAWFVAAAVLAFLFSFQKALSGWIAGIGGAVGSL
                     YTAAAGFTVLTGAVGVSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHA
                     QVKCNGLQINMLMAAAVCAVIASNLGMFVVMAEVMALCAVFLTSNSKEGKLWFALGRL
                     GTLLLVIACWLLWQRYGTLDLCLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWV
                     PQAHANASAPAAALFSTVVMKIGLLGMLTLSLLGGNAPLWWGIALLVLGMITAFVGGL
                     YALMEHNIQRLLAYHTLENIGIILLGLGAGVTGIALEQPALIALGLVGGLYHLLNHSL
                     FKSVLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEW
                     VIYQSFFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAEN
                     ATCAPLLMSVSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLI
                     ACPLLPFIIMAICKGDRLPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKL
                     RKWLNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            12760..13683
                     /gene="hycD"
                     /locus_tag="JNC24_000232"
     CDS             12760..13683
                     /gene="hycD"
                     /locus_tag="JNC24_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HAY9157090.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIFKLLGRQSVGPDASGWVFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTAGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWTGFGFAFLAFVSLLAA"
     gene            13701..15398
                     /locus_tag="JNC24_000233"
     CDS             13701..15398
                     /locus_tag="JNC24_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase large subunit"
                     /protein_id="HAY9157091.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDELYPLRKDSMDYRQRPAPTTD
                     AETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHRGMEK
                     LAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILLEVER
                     LHSHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIRRDLL
                     KDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEMARDFSNVGPMVRA
                     SGHARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLDNLPG
                     GPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPTLRYM
                     LRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNSPLK"
     gene            15408..15950
                     /gene="hycF"
                     /locus_tag="JNC24_000234"
     CDS             15408..15950
                     /gene="hycF"
                     /locus_tag="JNC24_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HAY9157092.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWEFNLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KEDFLQQSRFALCNCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            15950..16717
                     /gene="hycG"
                     /locus_tag="JNC24_000235"
     CDS             15950..16717
                     /gene="hycG"
                     /locus_tag="JNC24_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HAY9157093.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGIFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGEQDEQQAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDFLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEARIR"
     gene            16714..17124
                     /locus_tag="JNC24_000236"
     CDS             16714..17124
                     /locus_tag="JNC24_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HycH family protein"
                     /protein_id="HAY9157094.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDEYLAWIATLEAGSEARRKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQKEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            17117..17587
                     /gene="hycI"
                     /locus_tag="JNC24_000237"
     CDS             17117..17587
                     /gene="hycI"
                     /locus_tag="JNC24_000237"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HAY9157095.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPDDIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEEE"
     gene            17612..18385
                     /locus_tag="JNC24_000238"
     CDS             17612..18385
                     /locus_tag="JNC24_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001026459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9157096.1"
                     /translation="MNIRTGMCAFLLSLVLPAQATSFTEYLPMSDSEYAQKRALKPLL
                     TMPYDAEQTWRFRKVGVAGVTLEKMPNDDSEWQLNGKDRAGKSWSVPVGMLANIAGKG
                     QLYRADLDRNGIQDLVIWLPSTGNGLAPYAHLILMTFTREGRPCVFEPRGFYTASKTG
                     VDDLLDLQGNGHTQLLDMQFDSGYWITSLYQVKDAKWQRVHGWFGKLSYPALTRFTYT
                     PNRKLVLKPIAGRDPQTEDLAQTQRCLIKGDVLDGVNQN"
     gene            18547..18858
                     /locus_tag="JNC24_000239"
     CDS             18547..18858
                     /locus_tag="JNC24_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
                     /protein_id="HAY9157097.1"
                     /translation="MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTA
                     FPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRFFVKHITNHAEYDKLTRYYRENK
                     E"
     gene            18855..19274
                     /locus_tag="JNC24_000240"
     CDS             18855..19274
                     /locus_tag="JNC24_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAY9157098.1"
                     /translation="MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEE
                     DDTNPLIDFLASRIAEYENNNQEFAEFDKAVAAMPVGVALLRTLIDQHNLTYADLKNE
                     IGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL"
     gene            complement(19388..20812)
                     /locus_tag="JNC24_000241"
     CDS             complement(19388..20812)
                     /locus_tag="JNC24_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAY9157099.1"
                     /translation="MSVFPEGFLWGGALAANQSEGAFREGGKGLTTVDMIPHGEHRMA
                     VKLGLEKRFQLRDDEFYPSHEATDFYHRYKEDIALMAEMGFKVFRTSIAWSRLFPQGD
                     EITPNQQGIAFYRSVFEECKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVEFFSRY
                     ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASA
                     LATKIAHEVNPQNQVGCMLAGGNFYPYSCKPEDVWAALEKDRENLFFIDVQARGTYPA
                     YSARVFREKGVTIDKAPGDDEILKNTVDFVSFSYYASRCASAEMNANNSSAANVVKSL
                     RNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDELAANGEINDDYR
                     ISYLREHIRAMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVYVDRDDAGNGT
                     LTRTRKKSFWWYKKVIASNGEDLE"
     gene            complement(20821..22278)
                     /gene="ascF"
                     /locus_tag="JNC24_000242"
     CDS             complement(20821..22278)
                     /gene="ascF"
                     /locus_tag="JNC24_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
                     /protein_id="HAY9157100.1"
                     /translation="MAKNYAALARSVIAALGGVDNISAVTHCMTRLRVVIKDDQLIDS
                     PTLKTIPGVLGVVRSDNQCQVIIGNTVSQAFQEVVSLLPGDMQPAQPVGKPKLTLRRI
                     GAGILDALIGTMSPLIPAIIGGSMVKLLAMILEMSGVLTKGSPTLTILNVIGDGAFFF
                     LPLMVAASAAIKFKTNMSLAIAIAGVLVHPSFIELMAKAAQGEHVEFALIPVTAVKYT
                     YTVIPALVMTWCLSYIERWVDRITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGS
                     AISALVYTIHGYLGWLSVAIMGALWPLLVMTGMHRVFTPTIIQTIAETGKEGMVMPSE
                     IGANLSLGGSSLAVAWKTKNPELRQTALAAAASAIMAGISEPALYGVAIRLKRPLIAS
                     LISGFICGAVAGMAGLASHSMAAPGLFTSVQFFDPANPMSIVWVFAVMALAVVLSFIL
                     TLLLGFEDIPVEEAAAEARKHQSVQPTVAKEVSLN"
     gene            22538..23548
                     /locus_tag="JNC24_000243"
     CDS             22538..23548
                     /locus_tag="JNC24_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAY9157101.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESG
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRKNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALAQHGIALNEKLIA
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAMKALHERGVAVPEQVSVIGFD
                     DIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRDSLIAL
                     SR"
     gene            23696..24223
                     /gene="hydN"
                     /locus_tag="JNC24_000244"
     CDS             23696..24223
                     /gene="hydN"
                     /locus_tag="JNC24_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HAY9157102.1"
                     /translation="MNRFIIADASKCIGCCTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            24376..26628
                     /gene="hypF"
                     /locus_tag="JNC24_000245"
     CDS             24376..26628
                     /gene="hypF"
                     /locus_tag="JNC24_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HAY9157103.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLREDPETLLVQLHQHCPPLARIDSVEREPYIWSQLPTEFTIRQSAGGAMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRARKHRPAKPLAVMLPVADGLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPDLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLIHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALALPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYVV
                     HDAHPGYVSSQWAREMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCHGVTHPVTMPLVDNQLDLAAFWQQWLNWQAPVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(26756..27889)
                     /gene="norW"
                     /locus_tag="JNC24_000246"
     CDS             complement(26756..27889)
                     /gene="norW"
                     /locus_tag="JNC24_000246"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HAY9157104.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDASIPLTLIAADSMDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAGQFNLHLFPHTWVTDIDAEAHVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDHQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNDDIYALGDCAE
                     INGQVLPFLQPIQLSAMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFGLLKTLPM"
     gene            complement(27886..29325)
                     /gene="norV"
                     /locus_tag="JNC24_000247"
     CDS             complement(27886..29325)
                     /gene="norV"
                     /locus_tag="JNC24_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HAY9157105.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAVDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTKSDLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            29512..31026
                     /gene="norR"
                     /locus_tag="JNC24_000248"
     CDS             29512..31026
                     /gene="norR"
                     /locus_tag="JNC24_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HAY9157106.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFAGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMMPGDATPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VMAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRLGLSRVVLSAGAR
                     NLLQHYRFPGNVRELEHAIHRAVVLARATRNGDEVILEAQHFAFPEVTLPPPEAAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGMKD"
     gene            complement(31023..31988)
                     /gene="gutQ"
                     /locus_tag="JNC24_000249"
     CDS             complement(31023..31988)
                     /gene="gutQ"
                     /locus_tag="JNC24_000249"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HAY9157107.1"
                     /translation="MSEALLNAGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTSAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEILMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(31981..32754)
                     /gene="srlR"
                     /locus_tag="JNC24_000250"
                     /gene_synonym="gutR"
     CDS             complement(31981..32754)
                     /gene="srlR"
                     /locus_tag="JNC24_000250"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HAY9157108.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFSFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            complement(32821..33180)
                     /gene="gutM"
                     /locus_tag="JNC24_000251"
     CDS             complement(32821..33180)
                     /gene="gutM"
                     /locus_tag="JNC24_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HAY9157109.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVGR
                     SSGRFKPRVVVAIALDDQQRIVDTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            complement(33286..34054)
                     /gene="srlD"
                     /locus_tag="JNC24_000252"
                     /pseudo
     CDS             complement(33286..34054)
                     /gene="srlD"
                     /locus_tag="JNC24_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311588.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
     gene            complement(34058..34429)
                     /gene="srlB"
                     /locus_tag="JNC24_000253"
                     /gene_synonym="gutB"
     CDS             complement(34058..34429)
                     /gene="srlB"
                     /locus_tag="JNC24_000253"
                     /gene_synonym="gutB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAY9157110.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFTLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            complement(34440..35399)
                     /gene="srlE"
                     /locus_tag="JNC24_000254"
                     /gene_synonym="gutE"
     CDS             complement(34440..35399)
                     /gene="srlE"
                     /locus_tag="JNC24_000254"
                     /gene_synonym="gutE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HAY9157111.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSSPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSHFLTGAPTVLIAWFVSGFIYQ"
     gene            complement(35396..35959)
                     /gene="srlA"
                     /locus_tag="JNC24_000255"
                     /gene_synonym="gutA"
     CDS             complement(35396..35959)
                     /gene="srlA"
                     /locus_tag="JNC24_000255"
                     /gene_synonym="gutA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HAY9157112.1"
                     /translation="MIETITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNAL
                     INFIGQHRIERFAQRCAGNPVSRYLLLPCIGTFVFCNPMTLSLGRFMPEKYKPSYYAA
                     ASYSCHSMNGLFPHINPGELFVYLGIASGLTTLNLPLGPLAVSYLLVGLVTNFFRGWV
                     TDLTTAIFEKKMGIQLEQKVHLAGATS"
     gene            36215..37300
                     /gene="mltB"
                     /locus_tag="JNC24_000256"
     CDS             36215..37300
                     /gene="mltB"
                     /locus_tag="JNC24_000256"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HAY9157113.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDRMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAVMAN
                     GQAPGLPNGFKTRYSISQLATAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            37445..37942
                     /gene="pncC"
                     /locus_tag="JNC24_000257"
     CDS             37445..37942
                     /gene="pncC"
                     /locus_tag="JNC24_000257"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HAY9157114.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            38022..39083
                     /gene="recA"
                     /locus_tag="JNC24_000258"
     CDS             38022..39083
                     /gene="recA"
                     /locus_tag="JNC24_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HAY9157115.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            39311..39811
                     /gene="recX"
                     /locus_tag="JNC24_000259"
     CDS             39311..39811
                     /gene="recX"
                     /locus_tag="JNC24_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HAY9157116.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALVRDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WLNFAD"
     gene            39939..42569
                     /gene="alaS"
                     /locus_tag="JNC24_000260"
     CDS             39939..42569
                     /gene="alaS"
                     /locus_tag="JNC24_000260"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HAY9157117.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADVDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            42804..42989
                     /gene="csrA"
                     /locus_tag="JNC24_000261"
     CDS             42804..42989
                     /gene="csrA"
                     /locus_tag="JNC24_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HAY9157118.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            43305..43397
                     /locus_tag="JNC24_000262"
     tRNA            43305..43397
                     /locus_tag="JNC24_000262"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43339..43341,aa:Ser,seq:gct)
     gene            43401..43477
                     /locus_tag="JNC24_000263"
     tRNA            43401..43477
                     /locus_tag="JNC24_000263"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43435..43437,aa:Arg,seq:acg)
     gene            43541..43617
                     /locus_tag="JNC24_000264"
     tRNA            43541..43617
                     /locus_tag="JNC24_000264"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43575..43577,aa:Arg,seq:acg)
     gene            43816..43892
                     /locus_tag="JNC24_000265"
     tRNA            43816..43892
                     /locus_tag="JNC24_000265"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43850..43852,aa:Arg,seq:acg)
     gene            44173..44739
                     /gene="yqaB"
                     /locus_tag="JNC24_000266"
     CDS             44173..44739
                     /gene="yqaB"
                     /locus_tag="JNC24_000266"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HAY9157119.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDIQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVDVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRHYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
     gene            44736..45164
                     /locus_tag="JNC24_000267"
     CDS             44736..45164
                     /locus_tag="JNC24_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HAY9157120.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISHPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKAIGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            45237..46793
                     /gene="gshA"
                     /locus_tag="JNC24_000268"
     CDS             45237..46793
                     /gene="gshA"
                     /locus_tag="JNC24_000268"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000611798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HAY9157121.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLLQVGKDLFRDLKRVAQTLDSINGGDAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMETADTE
                     PFAVWLEKHT"
     gene            46943..47458
                     /gene="luxS"
                     /locus_tag="JNC24_000269"
     CDS             46943..47458
                     /gene="luxS"
                     /locus_tag="JNC24_000269"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HAY9157122.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILERDVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(47522..49060)
                     /gene="emrB"
                     /locus_tag="JNC24_000270"
     CDS             complement(47522..49060)
                     /gene="emrB"
                     /locus_tag="JNC24_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HAY9157123.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTKRRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDWFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(49077..50249)
                     /gene="emrA"
                     /locus_tag="JNC24_000271"
     CDS             complement(49077..50249)
                     /gene="emrA"
                     /locus_tag="JNC24_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
                     /protein_id="HAY9157124.1"
                     /translation="MSANAETQTPQQPVKKSGKRKRLLLLLTLLFIIIAVAIGIYWFL
                     VLRHFEETDDAYVAGNQIQIMSQVSGSVTKVWADNTDFVKEGDVLVTLDPTDARQAFE
                     KAKTALASSVRQTHQLMINSKQLQANIEVQKIALAKAQSDYNRRVPLGNANLIGREEL
                     QHARDAVTSAQAQLDVAIQQYNANQAMILGTKLEDQPAVQQAATEVRNAWLALERTRI
                     VSPMTGYVSRRAVQPGAQISPTTPLMAVVPATNMWVDANFKETQIANMRIGQPVTITT
                     DIYGDDVKYTGKVVGLDMGTGSAFSLLPAQNATGNWIKVVQRLPVRIELDQKQLEQYP
                     LRIGLSTLVSVNTTNRDGQVLANKVRSTPVAVSTAREISLAPVNKLIDDIVKANAG"
     gene            complement(50376..50906)
                     /gene="emrR"
                     /locus_tag="JNC24_000272"
     CDS             complement(50376..50906)
                     /gene="emrR"
                     /locus_tag="JNC24_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HAY9157125.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            50877..50978
                     /locus_tag="JNC24_000273"
     CDS             50877..50978
                     /locus_tag="JNC24_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9157126.1"
                     /translation="MFNGRKRTIHLGMTSLISADIVTTVTNKVNVLF"
     gene            complement(50997..51332)
                     /gene="ygaH"
                     /locus_tag="JNC24_000274"
     CDS             complement(50997..51332)
                     /gene="ygaH"
                     /locus_tag="JNC24_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HAY9157127.1"
                     /translation="MSYEVLLPGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            complement(51322..52059)
                     /locus_tag="JNC24_000275"
     CDS             complement(51322..52059)
                     /locus_tag="JNC24_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HAY9157128.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            complement(52183..53367)
                     /locus_tag="JNC24_000276"
     CDS             complement(52183..53367)
                     /locus_tag="JNC24_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY9157129.1"
                     /translation="MTKPNHELSPALIVLMSIATGQAVASNYYAQPLLDTIARNFSLS
                     ASSAGFIVTAAQLGYAAGLLFLVPLGDMFERRRLIVSMTLLAAGGMLITASSQSLAMM
                     ILGTALTGLFSVVAQILVPLAATLASPDKRGKVVGTIMSGLLLGILLARTVAGLLANL
                     GGWRTVFWVASVLMALMALALWRGLPQMKSETHLNYPQLLGSVFSMFISDKILRTRAL
                     LGCLTFANFSILWTSMAFLLAAPPFNYSDGVIGLFGLAGAAGALGARPAGGFADKGKS
                     HHTTTFGLLLLLLSWLAIWFGHTSVLALIIGILVLDLTVQGVHITNQTVIYRIHPDAR
                     NRLTAGYMTSYFIGGAAGSLISASAWQHGGWAGVCLAGATIALVNLLVWWRGFHRQEA
                     AN"
     gene            complement(53658..54650)
                     /gene="proX"
                     /locus_tag="JNC24_000277"
     CDS             complement(53658..54650)
                     /gene="proX"
                     /locus_tag="JNC24_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HAY9157130.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYCEGIFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWAEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            complement(54708..55772)
                     /gene="proW"
                     /locus_tag="JNC24_000278"
     CDS             complement(54708..55772)
                     /gene="proW"
                     /locus_tag="JNC24_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HAY9157131.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPTTAPTDGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNSFQQLLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            complement(55765..56967)
                     /gene="proV"
                     /locus_tag="JNC24_000279"
     CDS             complement(55765..56967)
                     /gene="proV"
                     /locus_tag="JNC24_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HAY9157132.1"
                     /translation="MAIKLEIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAKLRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            complement(57323..58282)
                     /locus_tag="JNC24_000280"
     CDS             complement(57323..58282)
                     /locus_tag="JNC24_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase 2 subunit
                     beta"
                     /protein_id="HAY9157133.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNANKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            complement(58292..60436)
                     /gene="nrdE"
                     /locus_tag="JNC24_000281"
     CDS             complement(58292..60436)
                     /gene="nrdE"
                     /locus_tag="JNC24_000281"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HAY9157134.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     MATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDATLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADIAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPVAGRINMSNLCSEIL
                     QVNCASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFARTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYAITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSGITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            complement(60409..60819)
                     /gene="nrdI"
                     /locus_tag="JNC24_000282"
                     /pseudo
     CDS             complement(60409..60819)
                     /gene="nrdI"
                     /locus_tag="JNC24_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
     gene            complement(60816..61061)
                     /gene="nrdH"
                     /locus_tag="JNC24_000283"
     CDS             complement(60816..61061)
                     /gene="nrdH"
                     /locus_tag="JNC24_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HAY9157135.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            complement(61309..61638)
                     /locus_tag="JNC24_000284"
     CDS             complement(61309..61638)
                     /locus_tag="JNC24_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HAY9157136.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     LLSMRKS"
     gene            61790..62134
                     /locus_tag="JNC24_000285"
     CDS             61790..62134
                     /locus_tag="JNC24_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgaC family protein"
                     /protein_id="HAY9157137.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            complement(62171..62620)
                     /gene="alaE"
                     /locus_tag="JNC24_000286"
     CDS             complement(62171..62620)
                     /gene="alaE"
                     /locus_tag="JNC24_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HAY9157138.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSHYQQVKA"
BASE COUNT        14826 a        16245 c        16982 g        14950 t
ORIGIN      
        1 atggcatatt ttatgttgtc attaatgatt tcaataatat gtggaaggtg atggaatttc
       61 agcaacagat ctattgcctc ttgttgctct gaatcattta aatcgaaaaa ccagtcacca
      121 ccgctggcaa gccacatatt tccatcgcca gtttcgaatg catccaacgc catcgcttcg
      181 tggttgcctt taaccgacgt aaaccagggt tggtttagca ggcgcagcac gttaagactc
      241 ttcggcccac gatcaatatt atcgcctgtg gaaataagta agtcggtttc agggtaaaaa
      301 gagagttgat gttaacggga ttgtaataac tgatattcac catgaatatc accaacgacc
      361 catatatggc gatagtgatg ggcattgatt ttttgatagc gtgtagatgg catggtttta
      421 ccctgtaaaa taagctttcc tattatacag ggtattttta tttgattcgt cagttgtcgt
      481 taatattccc gatagcaaaa gactatcggg aattgttatt acaccagact cttcaagcga
      541 taaatccact ccagcgcctg acgcggagtc agtgaatccg ggtcgagatt ttccagtgct
      601 tcgaccgcag gcgaagtttc ttctggcact gacagcaaag acatctgcgt accatccact
      661 tgcgtcgcgg cggcgttcgg cgaaatgctt tccagctcac gcagtttttg ccgtgcgcgc
      721 ttaataacct ctttcggtac gcccgccaga gcagcaaccg ccagaccgta gcttttgctc
      781 gccgcgccgt cctgcacgct gtgcataaag gcgatagtgt cgccgtgctc cagcgcatcg
      841 agatgtacgt tggcgacgcc ttccattttc tccggtagct gggtcagctc gaaatagtgg
      901 gtggcaaaca gcgtcaatgc cttaatctta ttcgccagat tttctgcgca cgcccacgcc
      961 agcgacagac catcgtaggt ggacgttcca cgcccgatct catccattaa caccagactg
     1021 tattcggtgg cgttatgtaa aatattggcg gtttcagtca tctccaccat aaaggttgag
     1081 cgcccggaag ccaggtcatc tgccgcgcct acgcgggtaa agatgcgatc gataggtcca
     1141 atctcgactt tttgtgccgg tacatagctg ccgatgtagg ccatcagcgc aatcagtgcg
     1201 gtctggcgca tataggtact tttaccgccc atgttcggac cggtgatgat caacatacgg
     1261 cgctgcggcg acagattcag cgggttagcg ataaatggct cattcagtac ttgttcaact
     1321 accggatggc gaccttcggt aatgcgaatg cccggtttat caatgaaggt cgggcaggtg
     1381 tagttcaggg tataggcccg ttccgccagg ttcaccagca cgtcgagttc cgccagcgcg
     1441 ctcgcgctct gttgcaacgc ttccagatgc ggcaacagca ggtcgaacaa ctcttcataa
     1501 agctgttttt ccagtgccag tgctttgcct tttgaggtga gaactttatc ttcgtactct
     1561 tttagctctg gaatgatgta gcgctcggcg tttttcagcg tctggcgacg catgtagttg
     1621 atgggtgcca gatggctttg cccacggctg atttgaatgt agtagccgtg caccgcatta
     1681 aagccaactt tcagcgtgtc caggccggta cgttcacgct cgcggacttc cagacgctcc
     1741 agataatcgg tcgcgccgtc agccagcgcg cgccactcat ccagctcttc gttatagccc
     1801 gatgcgataa caccaccgtc gcgtaccagc accggcggtg tgtcgatgat tgctcgctcc
     1861 agcagatcgc gcagctcggc aaactcgccc atcttctcac gtagcgcctg taccggtgca
     1921 ctatcgacag tttctaactg cgcacgcagc tccggcagtt gctggaaagc gtggcgcata
     1981 cgggccagat cgcgtgggcg agcagttcgt aaagccagac gtgccagaat acgttccagg
     2041 tcgccgacct gacgcagtac cggctgcaac tcggcggtga aatcctgcaa tgcgccaata
     2101 gtttgctggc gctcaagcaa cacgcgggta tcgcgcactg gcatatgcag ccagcgtttc
     2161 agcatacgac tacccatcgg cgtgacggtg cagtcgagca cagaagccag cgtattttcc
     2221 gcaccgcccg ccaggttctg agtaatttcc aggttacgac gtgtcgcggc atccataatg
     2281 atgctgtcct gctcacgttc catggtgata gaacgaatat gcggcagggt cgtgcgttgg
     2341 gtatctttcg catactgcaa cagacaaccg gcagcacaaa gtccgcgcgg cgcgttctcg
     2401 acgccaaaac cgaccagatc gcgggtgcca aattgcagat tcaactgctg gcgcgcggtg
     2461 tcgatttcaa actcccacag cgggcgacgg cgcaggccgc gacggccttc aatcagcgac
     2521 atctcggcga aatcttctgc atacagcagt tccgccggat tagtgcgttg cagctctgcc
     2581 gccatcgttt cgcggtcggc cggttcgctc aggcgaaaac gcccggagct gatatccagc
     2641 gtcgcgtagc cgaaaccttt gctgtcctgc cagatagccg ccagcaggtt gtcctgacgc
     2701 tcctgtaaca gggcttcatc gctgatggtg cctggcgtaa cgatacgcac aactttgcgc
     2761 tcaaccggac ctttgctggt cgccggatcg ccaatttgtt cgcagatggc aacggactct
     2821 ccctgattca ccagtttggc gagatagttt tccaccgcat ggtagggaat ccccgccatc
     2881 gggatcggct ctcccgccga agcaccgcgt ttggtcagtg aaatatccag cagttgcgac
     2941 gcgcgttttg cgtcgtcata aaacagttca taaaaatcac ccatccggta aaacagcagg
     3001 atctcgggat gctgggcttt cagcctgaga tactgctgca tcatgggcgt atgggcgtcg
     3061 aaattttcta ttgcactcat ggggttatgt ccggttccct gatattaaat ggggtgtgat
     3121 ggtttttatt ttcgttgtaa tcacataagg cgcacatgat accggagtta atcatttcag
     3181 gggaagaagc gaacggcgag ttcttgatgc tggttccggg aaagatgctg ttttgcagct
     3241 tttgcgtgat gcttatgaag caagtacgca aaattgcgtc ggttgaataa acctcctcct
     3301 tcagagccat agtccccttt caaattaagt tgtaatataa atacacctta agagtatgga
     3361 ggcagcatgt ccggcaagcg tatctctcgt gaaaaactga cgattaaaaa aatgatcgat
     3421 ctttatcaag cgaaatgccc gcaggcgtca gcggagccgg agcattacga ggcgttgttt
     3481 gtttacgcgc aaaagcggct ggataaatgt gtgttcggcg aagagaaacc agcctgtaag
     3541 cagtgtccgg tgcactgtta ccagcctgca aagcgtgaag agatgaaaca gattatgcgt
     3601 tgggccgggc cgagaatgct gtggcgtcat ccgatcttaa ccgtgcgtca tctcatcgat
     3661 gacaagcgtc cggtaccaga actgccggaa aaatatcgtc cgaaaaaacc tcgtgaatag
     3721 tatgccggac aggcagatct gtccggcaat ttgcagttaa atcaatgccg atttatcaat
     3781 tcccagccgc ttcatccgtg acagcagggt cgtgcgtttc agccccagac gttgcgcagc
     3841 gcctttaggc ccggcaacca cgccgttggt ttctttcagc acgcgcacaa tcaactgata
     3901 ttcatcttcg ccctcctggg cgacaaccgt tgcggcaggc ggcgtttcag gttccggtaa
     3961 aacaatatct ggcaatgaca gctgcagcac gttaccgcgt gttagcaata ccgcgcgctc
     4021 aatgacgttt tccagttcgc gtacgttacc cggccactcc atgttgctca aggtgcgcag
     4081 cgtctcggca ggaatgctgt cgatattgcg ccccagacga cgggcaattt tgaaggtaaa
     4141 ggctttcgcc agcagcggaa tatcttccgg acgctcgcgt agtggcggca ggtgaatcgg
     4201 gaatacgttc aggcggtaat agagatcgct acggaactct cggtcggcga ccattttttt
     4261 cagatcgcgg ttagtcgcgg caattaaacg tacgtccgtc tgaatgattt tgttgctgcc
     4321 gagacgttca aactcctgtt cctgcaatac acgcagcaac ttcggctgta actccagtgg
     4381 catatcgccc acttcgtcga ggaacagaga gcttttatcc gccagttcaa aacgaccaat
     4441 gcgctgggcg ctggcaccgg taaaagcccc acgctcatga ccaaacagat cgctttccag
     4501 caatccggca ggcatcgccg cgcagttcat tttgaccatg cggcgattat tacgcccact
     4561 gagattatgg atcgcgcggg caatcagctc tttacccgtg ccagtttcac cgaggatcag
     4621 cacggtactg tcactttgcg ccaccatttc aacttgttta agcacgctgt acatggcttc
     4681 gctgcggcca ataatctcgc caaattcact atcaacattg ttgagctgct cggtcagggc
     4741 gaggttttca tcaaccagcc gttctttcag acgatggatt tcctgatagg cgagggcgtt
     4801 atcgacagcg attgccacac gttcggcaat ctggcgcagc aaattcagat tggtagtggt
     4861 aaacactttc tcttcgcatt gcgccagttt cagcacgccc agcatggtgt cgccagacat
     4921 cagcggtaac aggcacaagg tttgaatctg gttgccctag gtgtcgaaca acatgcgttc
     4981 atagggggct aaatcgtccc gctcgtggag attgatcagc agcatctctt tacttttgaa
     5041 cacgcgttcg gtgagggttc cggcttcatc gacttcgctc tgttcgtggg cgggatgttg
     5101 tttatcaaga tagtgagtgg agtagatgtt gagtttgttt ttacggtggc tgcgtaagac
     5161 gatactgata tcgtcaatgt cgaaatagta atggatttct ttggcgactt cgctgaccag
     5221 ttcgtccata tccaggcggg aaagcaccgc gttggtaatg gcaaccagga tgcggaagtt
     5281 atcgcgctcc cggcataaca actcatagtc gacattgttg acgacgcggc tctggatttg
     5341 ttcggtgacg acagaaacga tctgcgtaaa tgtttgcaga cgattgaact ctttttcgct
     5401 ccagggacga tcgtcataac gaataaattc acagccacca aaaatatgcc cttccgccgc
     5461 cagtggcatc aggcaatagt gaccaaattt ggggtatagc ccacctgcga ccagctgcgg
     5521 ccaggtttca caaaattctt cgtaactgca atgcagcgta tcagggcgcg acaaaatgcg
     5581 gcgtaccgga ccgtgtgcca gaacagtttc gtcttcatat ttaatggggt gtctttttca
     5641 cgcgatgcgt aataagacgc acgttgagtc tgcgcttgcc acaacacaat agccgcgttg
     5701 tcggcgagcg cagaacgctt taccagttgc gaaagagcct cacacagcga ggccagatcg
     5761 ggctgctgca atagtgtccg agtgatgtcg aacaaccctt gctgtccgag atcactcatc
     5821 ggtgtatatg acattttgtt cttccaggaa agcaccgcaa agcggtgcaa aaggtcattg
     5881 actaactata ggagatttag aattttatta gcatatacgc ggaagaggtt cggcgtgtgg
     5941 taaatcgagg gttcgtttca caccatacag accggcaaga cgaacacctt tacgttccac
     6001 cacttcacca attagcgccg cgtctttccc cagtggatgg gaatgtaacg ctgccagcac
     6061 ttgctctgcc gcgttgcgtt caacggcgat caccagtttg ccttcgttgg caaagttaag
     6121 ggcgtccagt cccagcagtt cgcaaacgcc gcgcacggca ggtttaaccg gcagtgccac
     6181 ttctgaaatt tcaataccac aaccgcaggc tgccgcgaac tcatgaacta ccgcgttcac
     6241 accaccacgg gtggcatcac gcagcgcttt cacgccggga atgtcacgca gcgtctgaat
     6301 aagcggcgtc agtaccgcgc agtcgctgac cagttcgcca tccagcccca gctgctcacg
     6361 caggttaagg atagtcgccc cgtggtcccc gagcgtgccg ctaaccagta atacatcgcc
     6421 tgcggttagc gtctgtgcgc cccagtgaat attcgccgga attgcgccca tgccagcggt
     6481 gttgataaac agtttatctg ccgcgccgcg ctgcaccact ttagtatcgc cagtaacgat
     6541 ggcaatgcct gccgcgcgcg cggtttctgc catgctggtc actacggctt tcagtgtctc
     6601 catcggcaat ccttcttcga ggataaagcc acaggagaga tagcgcggaa tagcgccact
     6661 gaccgcaacg tcattggctg tgccgcaaat cgccagcttg ccgatattac cgccaggaaa
     6721 gaacagcggg tcaatgacgt aactgtcggt ggagaacgcc agacggtcgc cttctgctac
     6781 cagctgcgcc agatcaagac gtgcctggtc ttcctgctct gccagccacg ggttggcaaa
     6841 ggcttccata aacaggctgt tgattaattg ctgcatcgcc tggccgccgc tgccgtgggc
     6901 gagttggata ttattcacgc ttcattctcc tgctgacgat actgatacca cgcggcgcac
     6961 gctccttcgg aggagaccat cagtgcacca aatgcggttt gaggattaca ggtgttacca
     7021 aacagcgggc attgatgcgg cttacatttg cccgttaata cctcaccaca acgcgcgcgc
     7081 gggtcatcgc agacctgctg cggtgctggg cggaaatgtg cttcggcatc gaatcgttga
     7141 taatccggcg tcaggtgcac gccagaagat tcaatcacgc ctaagccgcg ccattcgctg
     7201 tcgccgttga cacagaacac atcagcaatt gcctgttgcg ccagcaggtt accggcatcc
     7261 ggtaccaccc gacgatactg attctctacc ttgctgtggg ccgctatttt ctgctccacc
     7321 agcatgacca cgccttgcag tagatcaagg ggttcgaaac cagcgaccac cagcggacga
     7381 tgaaaatcgc tggcgataaa attataggca tcagtgccga taaccatact aacgtggccc
     7441 ggcgcgagga acgcgtcgat accgttatcc ggctgttcca gcaaactgcg cagcgtcggg
     7501 ataagcgtaa tatgctggca gaagaagtaa aaattctgca catcgcgggc tttcgcctgt
     7561 tgcagggtga tggcggtggt cggcatggtg gtttcaaaac ctaagccgaa gaatacgact
     7621 ttacgggttg gattctcctg cgccagtttc aacgcatcca tcggcgagta aacgatgcgc
     7681 acatcggcac cgcgtgcttt tgcctgcaac agcgatccct gtttccccgg cacgcgcatg
     7741 gcgtcgccaa aggtacagaa gatgacttcc ggatggctgg caatctccac gcaggtgtcg
     7801 attctaccca tcggcagtac gcacaccggg caacccggac cgtggataaa ctcaacgttt
     7861 tcaggcagta actggtcgag gccgaattta aaaatggcgt gggtatgacc accgcacact
     7921 tccataatcc gcagagggcg ttcggcggtg taagagagat gtgaagcacg ttcgcgcaga
     7981 tgctcaatta actgcatcac ctgttccggc gcgcgatatt catcaacaaa acgcattatt
     8041 tttcctcgcc atacaacagc gcgccgacat ccggctcaac gtcaaacatg ttttgtaagg
     8101 cgtcgagagt gtcgcgtgct tcggcttcat taattacgct catggcaaag ccaacgtgta
     8161 ccagtaccca ctggcccacg cgcggctgac cgttttcatc gcagctgccg actaacgtta
     8221 aatcgacatc gcgctgaatg ccgcagacgt cgacttttgc ctggttgccg tcaatggtgc
     8281 ggatctggcc gggaacgcct atgcacatcg ctgtgtctcc agccagttca gccactggtc
     8341 catcccttcg ccgctggtgg cggaaataag gatgatttca atttctggat tgacttcgcg
     8401 ggcgcaggcg atgcacttct caacgtcaaa gttgagatac ggcaacaggt caactttgtt
     8461 gagcagcatc agcgaggcgg cggcaaacat atgcggatat ttcagcggtt tgtcttcacc
     8521 ttcggtaacg gaaagcaccg ccactttgtg tttttcaccg agatcgaagc tggccgggca
     8581 tacgaggttg ccaacgtttt cgataaacag aataccgtta tcgtccagtg gcagacgcgg
     8641 tgcggcgtcg gcaatcatct gtgcgtcaag atggcagcct ttaccggtgt tcacctgaat
     8701 cgctggtgtg ccggtagcgc gaatgcgtgc ggcatcgttc acggtttgct ggtcaccttc
     8761 aataaccgcg cacggaacgc tgtctttcag gcgcattagg gtttccgtca gcagggtggt
     8821 tttaccggaa ccagggctgg aaaccaggtt gagcaccagt tgcttgcggg cagcaaagcg
     8881 cgcgcggttg cgttcagcca gacggttatt tttgtccagc acgtcaattt cgacttccag
     8941 catccgacgc tggctcatgc ccggcgcgtg agtgcccgct tcgccatgac cgtaatgcag
     9001 gtcgccttct tcagtctggc tgggggtaaa ttcaggcgct ttaatgccgg tgattttcat
     9061 cttcgggcgt gccgccgggg caaatggcgc gctacgaaac gcggaatgag ggttatgttc
     9121 atcaccctcg atatacaggt tgccttcacc gcaaccgcat gttgtacaca tcgctcactc
     9181 ctggtctatt tctatccgcc gaatctgtaa accgtcgtct gccacaatct gtagcatgtc
     9241 accatgacac tgtggacagc ggcggacgcg ctgggtcagt agcgtcacat actgttggca
     9301 tgtttcacac cagcattcgg cctcttgttc ttcgaggtgc agtttacaac cttccgccac
     9361 gctgccgcgg caaaccagat caaaacaaaa ggcaagagag ctggtttcga cacaagaaaa
     9421 tgcgccaatt ttgagccaga ccccagttac gcgttttgcg ccgtgttttg cggcctgctg
     9481 ttcgatcaat tccagtgccc gttggcagag ggttatttcg tgcatatcgc ctcccattaa
     9541 ctattgccag ctacaagcaa taattgtgcc agtgttgatt atccctgcgg tgaataatgt
     9601 cgatgatgtc gaaatgacac gtcgacacgg cgacgaaatt catctttagc ttaaaaatct
     9661 atttaataac aataaattaa aagttggcat agaaaatgct taaagctggc atctctgtta
     9721 aacgggtaac ctgacaatga ctatttggga aataagcgag aaagccgatt acatcgcaca
     9781 gcggcattgt cgcctacagg accagtggca catctactgc aattcgctgg ttcaggggat
     9841 cacgttatcg aaagcgcgcc tgcatcacgc catgagctgc gcgccggaca aagaactctg
     9901 tttcgtcctt tttgaacatt ttcgtattta cgtcaccctg gcggatggct ttaacagcca
     9961 caccatcgag tattacgtcg aaacaaaaga tggcgaagac aaacagcgga ttgcgcaggc
    10021 gcaactgagc attgacggca tgattgatgg caaggtcaac atccgcgatc gcgaacaggt
    10081 tctggaacac tatctcgaaa aaatcgctgg cgtttacgac agcttataca ccgctattga
    10141 aaacaatgtg ccggtgaatt taagccaact ggtaaaggga caaagcccgg cagcatgagc
    10201 tgaggctttg cccgttttgc aggcgttacg cctgtttggg gatgggcgtg tcgatgagtg
    10261 tcgaaaatga catttcatcg gcatgttttc gtcaaaaatg acaatcacct gaggaatgcc
    10321 tggtgaatcg ttttgtaatt gttgactcca cgctctgtat cggctgccac acttgtgagg
    10381 ccgcctgttc agagacgcat cgccagcacg gcctgcaatc aatgccgcgc ctgagagtga
    10441 tgctgaatga aaaagaatct gcgccgcagc tctgtcacca ctgtgaagat gcaccctgtg
    10501 cggtggtttg cccggttaac gccatcaccc gcattgatgg ggccgtgcag ttgaatgaaa
    10561 gtctgtgcgt aagctgcaaa ctgtgcggca tcgcctgccc gtttggcgca attgaatttt
    10621 ccggcagccg tccgctggat attccggcaa acgccaatac cccgaaagcg ccaccggcac
    10681 cgcctgctcc ggcgcgtatc agtacgctgc ttgactgggt gccgggtatt cgcgcgatcg
    10741 ccgtcaaatg tgacctttgc agttttgatg aacaaggtcc ggcctgcgtg cggatgtgcc
    10801 cgactaaagc cctgcatctg gtggataaca ccgatatcgc ccgcgtcagc aaacgtaagc
    10861 gtgagctgac ctttaacacg gactttggcg atctcacctt gtttcagcag gctcaaagtg
    10921 gagaggctaa atgagcgcaa tttccctgat caatagcggc gtggcatggt ttgtcgccgc
    10981 cgctgttctg gcatttctct tttcttttca aaaagcgtta agtggctgga tagctggaat
    11041 tggcggcgcg gttggtagtc tgtatacggc agccgcgggc ttcactgtac tgactggcgc
    11101 ggttggcgtg agcggtgcgc tgtcgctggt aagctacgat gtgcaaatct ctccgcttaa
    11161 cgcgatttgg ctgattacgc tcggtctgtg cggtctgttt gtcagcctct acaacattga
    11221 ctggcatcgc cacgcgcagg tgaagtgcaa cggcttgcag atcaatatgt tgatggctgc
    11281 cgccgtctgc gccgtcatcg ccagcaacct cggcatgttc gtggtaatgg ccgaagtcat
    11341 ggccttgtgt gcggtgttcc tcaccagcaa cagcaaagag ggcaaactgt ggtttgcgct
    11401 ggggcgtctt ggcactctgc tgctggtgat tgcttgctgg ctgctgtggc agcgttatgg
    11461 cacgctggat ctgtgcctac tggatatgcg tatgcaacag ctgccgctcg gttccgatat
    11521 ctggctgctc ggtgtgattg gctttggcct gctggccggg attattccgc tgcacggctg
    11581 ggtgccgcag gcacatgcta acgcctctgc gccagctgcc gcgttgtttt ctacggtagt
    11641 catgaaaatt ggcctgctgg gcatgttaac cctgtcactg ctgggcggta atgcaccgct
    11701 gtggtggggg atcgcgctgc tggtgctcgg catgatcacc gcgttcgtcg gtggtctgta
    11761 tgcgttgatg gaacacaaca tccagcgcct gctggcttac cacaccctgg aaaatatcgg
    11821 cattatcctg ctggggctgg gcgctggcgt aacgggtatc gcgctcgaac aaccggcgct
    11881 gattgctctt ggcctggtcg gtggtctgta ccatctgctt aaccatagcc tgttcaaaag
    11941 cgtactgttc ctcggggcgg ggagcgtctg gttccgtacc ggtcatcgcg atatcgaaaa
    12001 actcggtggt attggcaaga aaatgccggt tatctccatc gccatgttag tcgggttgat
    12061 ggcaatggct gcgctgccgc cactgaacgg gtttgccggg gaatgggtta tctatcaatc
    12121 cttcttcaaa ctgagcaata gcggcgcgtt tgttgcccgt ctgctggggc cgctgctcgc
    12181 tgtgggtctg gcaattaccg gtgcgctggc ggtgatgtgt atggcgaaag tctatggcgt
    12241 cacgttcctc ggcgcgccgc gtaccaaaga agccgaaaac gccacctgtg cgccgctcct
    12301 gatgagcgta agcgtagtgg cactggcgat ttgctgcgta attggcggtg ttgctgcgcc
    12361 gtggctgctg ccgatgctct ctgctgctgt acctctgcct ctggagcctg ctaacaccac
    12421 cgtttctcaa ccaatgatca cattgctgct gattgcctgc ccgctgctgc cattcatcat
    12481 tatggcgatt tgcaaaggcg accgcctgcc gtcacgttcc cgcggtgcgg cctgggtgtg
    12541 cggctacgac catgaaaaat caatggtgat taccgctcac ggttttgcca tgccggttaa
    12601 acaggcgttt gcgccagtgc tgaaactgcg caaatggctg aatccggtgt cgctggtgcc
    12661 gggctggcag tgcgaaggta gtgcgttgct gttccgccgg atggcgctgg ttgaactggc
    12721 ggtactggtg gtgattattg tttcacgagg agcctgagaa tgagtgtttt atatccgtta
    12781 attcaggcgc tggtgttatt tgccgttgcg ccgctgctct ccggtattac ccgcgtggcg
    12841 cgcgcccgtc tgcataaccg tcgtgggccg ggtgtgttgc aggagtatcg tgacattttc
    12901 aaactgctgg gccgccagag cgttggcccg gatgcctccg gctgggtgtt ccgcctgacg
    12961 ccgtatgtga tggtgggcgt catgctgact atcgctactg cgctgccggt ggtgaccgtc
    13021 ggttctccgc tgccgcaact gggtgattta atcaccttac tgtatctctt tgccatcgcg
    13081 cgtttcttct ttgccatttc tggtctggat accggtagcc cgtttaccgc tatcggcgcg
    13141 agccgtgaag cgatgcttgg cgtgctggtc gaaccgatgc tgctgcttgg tctgtgggtt
    13201 gccgcacagg ttgccggttc caccaacatc agcaacatca ccgacaccgt ttatcactgg
    13261 ccgctgagcc agagcatccc gctggtactg gcgctttgtg cctgtgcgtt cgccaccttt
    13321 atcgaaatgg gcaaactgcc gttcgacctg gcggaagccg agcaggagct gcaggaaggc
    13381 ccgctctctg aatacagcgg cagcggcttt ggcgtcatga aatggggtat cagcctgaaa
    13441 cagctggtgg tgctgcagat gttcgtcggc gtatttatcc cgtggggaca gatggaaacc
    13501 ttcaccgccg gtgggctgct gctggcgctg gtgattgcca tcgtcaaact ggtcgtcggc
    13561 gtcctggtta tcgcgctgtt cgaaaacagc atggcccgtc tgcgtcttga tattactccg
    13621 cgcattacct ggactgggtt tggctttgca tttttagcgt tcgtctcctt gctggcggcg
    13681 tgattaaaga gagtttgagc atgtctgaag aaaaattagg tcaacattat ctcgccgcgc
    13741 tgaatgaggc atttccgggc gtcgtgctgg accacgcctg gcagaccaaa gatcagctga
    13801 ctgtcaccgt aaaggtgaac tatctgccgg aagtggtgga gtttctctac tacaagcagg
    13861 ggggctggct gtcggtgctt tttggtaacg acgaacgcaa actgaatggt cattacgccg
    13921 tttattacgt gctgtcgatg gagaagggga ctaagtgctg ggtaacggtt cgcgtcgaag
    13981 ttgatgccaa caaaccggag tatccgtccg tgacgccgcg cgttccggcg gcggtgtggg
    14041 gcgagcgcga agtgcgcgat atgtacggtt tgattccggt tggtctgccg gatgaacgcc
    14101 gtctggtgct gccggatgaa ctttatccgc tgcgtaaaga cagcatggat tatcgtcagc
    14161 gtccggcacc gaccaccgat gctgaaacct acgagttcat caacgaactg ggcgacaaga
    14221 aaaacaacgt cgtgccgatt ggtccgctgc acgtcacttc tgacgaaccg ggccacttcc
    14281 gtctgttcgt cgatggcgaa aacattatcg acgccgacta ccgtctgttc tacgttcatc
    14341 gcggcatgga aaaactggcg gaaacccgta tgggttacaa cgaagtgacc ttcctctctg
    14401 accgtgtgtg cgggatctgc ggctttgccc acagcaccgc ctacaccacg tcggtagaaa
    14461 acgcgatggg tattcaggtg ccagaacgtg cgcagatgat ccgcgccatt ctgctggagg
    14521 tggaacgcct gcactcgcat ctgctcaacc tcggtctcgc ctgccacttt accggctttg
    14581 actccggctt tatgcagttc ttccgcgtgc gtgaaacctc catgaaaatg gcagagatcc
    14641 tcaccggtgc gcgtaaaacc tacggcctga acctgatcgg cgggattcgt cgcgatctgt
    14701 tgaaagatga catgatccag acccgccagc tggcgcaaca gatgcgtcgt gaagtgcagg
    14761 agctggtgga tgtgctgctg agtacgccga acatggaaca gcgcaccgtc ggcattggtc
    14821 gtctggaccc ggaaatggcc cgcgatttca gtaacgttgg cccgatggtt cgcgccagcg
    14881 gtcacgcccg cgatactcgc gccgatcacc cgtttgtcgg ttatggcctg ctgccaatgg
    14941 aagtccacag cgagcagggc tgcgacgtta tttcccgtct gaaagtgcgt atcaacgaag
    15001 tttataccgc gctgaacatg atcgactacg gtctggataa cctgccgggc ggtccgctga
    15061 tggtggaagg ctttacctac attccgcacc gtttcgcgct gggctttgcc gaagcgccgc
    15121 gcggcgacga tatccactgg agcatgaccg gcgacaacca gaagctgtac cgctggcgct
    15181 gccgtgccgc gacctacgcg aactggccga ccctgcgcta catgctgcgc ggtaacaccg
    15241 tttccgatgc gccgctgatt atcggtagct tggacccttg ctactcctgt accgaccgca
    15301 tgaccgtggt cgatgtgcgt aagaaaaaga gcaaagtggt gccgtacaaa gaactcgagc
    15361 gttacagcat tgagcgtaaa aactcgccgc tgaaataagg aatcgccatg tttaccttta
    15421 tcaaaaaagt catcaaaacc ggcacggcga cctcgtctta tccgctggag ccgattgcgg
    15481 ttgataaaaa cttccgtggt aagccggagc agaacccgca gcagtgcatc ggctgtgcgg
    15541 cctgcgtcaa tgcctgcccg tcaaacgcct taacggttga aactgacctc gccaccggtg
    15601 agctggcgtg ggagtttaat ctcgggcgct gcatcttctg tggacgctgc gaagaagttt
    15661 gcccgacggc ggcgatcaaa ctgtcgcaag agtacgaact ggcagtgtgg aagaaagaag
    15721 acttcctgca acagtcccgc ttcgcgctgt gcaactgccg cgtctgcaat cgtcctttcg
    15781 ccgtccagaa agagatcgac tacgccattg cgctgcttaa gcacaacggc gacagccgcg
    15841 cggaaaacca ccgcgaaagc tttgagactt gcccggaatg taagcgccag aaatgcctgg
    15901 tgccgtccga ccgtattgaa ctgactcgcc atatgaaaga ggccatctga tgagcaattt
    15961 attaggcccc cgtgacgcca acggcattcc ggtcccgatg acggtggatg aatccatcgc
    16021 cagcatgaag gcgtcgttac tgaaaaaaat caaacgttct gcctacgttt atcgcgtgga
    16081 ctgcggcggc tgcaacggct gcgaaatcga aattttcgcc acgctttcgc cgctgtttga
    16141 tgcagaacgc ttcggcatta aagtcgttcc ttcaccgcgt catgcggata ttttactgtt
    16201 taccggcgcg gtcacccgtg caatgcgatc ccctgcgctg cgtgcgtggc agtccgcgcc
    16261 ggacccgaaa atttgtatct cctacggtgc ctgcggtaac agcggtggta ttttccacga
    16321 tctctactgc gtgtggggcg gtacggataa aatcgtcccg gtagatgtct acattcctgg
    16381 ttgcccgcca acgcctgccg ccacgctgta cggctttgca atggcgctcg gcctgctgga
    16441 gcagaaaatt cacgcccgtg gcccgggtga gcaggatgaa caacaggcgg aaatcctgca
    16501 tggcgatatg gtgcagccgc tgcgtgtgaa agtggatcgc gaagcacgcc gcctggcggg
    16561 ttatcgttac ggtcgtcaga ttgccgatga cttcctgaca cagttagggc agggcgaaga
    16621 acaggttgca cgctggctgg aagcggaaaa cgatccgcgt ctgaacgaga ttgtcagcca
    16681 tctgaatcat gttgttgaag aggcgcgtat ccgatgagtg aaaaggtggt gttcagtcaa
    16741 ctgagccgta aatttattga tgagaacgat gccacgcccg ccgaggcgca gcaggtggtc
    16801 tattacagcc tggcgattgg tcaccacctt ggggttatcg attgcctgga agcggcgctc
    16861 acctgcccgt gggatgaata tctggcatgg attgccactc tggaggcggg cagtgaagcc
    16921 cgccgcaaaa tggaaggcgt gccgaaatat ggtgagatcg tcatcgacat taaccatgtg
    16981 ccgatgctgg ccaacgcatt cgataaagcc cgggcagcgc aaacttcgca gcagaaggaa
    17041 tggagtacaa tgctgttaag tatgctgcat gatattcatc aggaaaacgc catctatttg
    17101 atggtgagga gactgcgtga ctgacgtttt actctgtgtt ggcaatagca tgatgggcga
    17161 tgatggcgct ggcccgctgc tggcggaaaa gtgcgccgcc gcgccgaaag gtaactgggt
    17221 ggtgattgat ggcggtagtg caccggaaaa cgacatcgtc gccatccgtg aactgcgccc
    17281 tacgcggtta ctgattgttg acgctactga tatggggcta aaccccggcg aaatccgcat
    17341 catcgacccg gatgatatcg ccgagatgtt tatgatgact acccataaca tgccgctgaa
    17401 ctatcttatc gaccagttga aagaagacat tggcgaagtg attttcctcg gcatccagcc
    17461 ggatatcgtc ggcttttact acccgatgac tcagccgatt aaagatgcgg tagaaaccgt
    17521 ttatcaacga ctggaaggct gggaagggaa tggcggcttc gcgcagttag cggtggaaga
    17581 agaataattc tccatcaggg aattcagcga aatgaatatc agaacaggga tgtgcgcttt
    17641 tttattgagc ttagtgctgc ctgcacaggc caccagcttt accgaatatc tgccaatgag
    17701 tgacagcgaa tacgcgcaaa agcgggcgct gaaacctctc ctgaccatgc cttatgatgc
    17761 cgagcaaacc tggcgtttta gaaaagtggg cgttgctggc gtgacgcttg aaaagatgcc
    17821 aaatgatgat agtgaatggc aattgaacgg taaagatcgc gccggtaaat cctggtctgt
    17881 gcctgtgggt atgctggcaa acatagcggg caaagggcaa ctctatcgcg ccgatcttga
    17941 tcgtaacggc attcaggatc tggttatctg gttaccaagt accggtaatg gtctggctcc
    18001 ttatgcacat ttaattctta tgacgttcac gcgcgaaggc cgtccctgtg tctttgaacc
    18061 caggggattc tacaccgcaa gcaaaactgg cgtagatgat ttgctggatt tgcaaggcaa
    18121 tggtcacacg caattgctgg atatgcagtt tgattccggt tactggataa ccagtctgta
    18181 tcaggtaaaa gacgccaaat ggcagcgtgt tcacggctgg tttggcaagt taagctatcc
    18241 cgcgctcacg cgttttactt atacccccaa ccgtaaactc gtcctcaaac ccatcgccgg
    18301 gcgcgacccg caaacggaag atctggcgca gacgcagcgt tgcttgataa agggggatgt
    18361 tcttgatggg gtgaaccaga actgactttt tgcagagtgg ctatgaggat tttgggggta
    18421 attatatcgc gctcaagtgt ttacatattg tgaacttgga tttgatgagc tatagtttcc
    18481 gaacacataa ggatagaact cgcagatgtg gctgtttact tactataagg aagttaaaac
    18541 ttaaaaatgc acatcatttc taaagcgcca tttgaagagt ctgcgcgtaa atatcctaac
    18601 gacgctctgg ctcttcaggc attgtatcgg gttattaagg aaacggattt ttctacgcca
    18661 gaagagatgc gaacggcgtt tcccaacctc gacaatttta aatatcgcaa caaatggtac
    18721 gttctggatg taggtgggaa caacctaagg gtgattgcct acataaactt tgttaataag
    18781 cggttctttg tgaagcacat aactaatcac gccgagtatg acaaactgac acgttactac
    18841 agagagaaca aagaatgact gctaatgctg cacgcgctgt taaagcaaca agagaactgg
    18901 tcaatgcagt cccctttctg ggaggtagtg actcagaaga tgattatcgt gaagcactgg
    18961 aactggtcga atatttgatt gaagaagatg acaccaatcc acttatcgac tttctggcga
    19021 gccgcattgc agaatatgaa aacaacaacc aagagtttgc agagttcgac aaagccgttg
    19081 cggcaatgcc tgttggcgtg gcgttacttc gcacgctgat tgatcaacat aatctgacct
    19141 atgcggattt gaagaacgaa atcggctcta aatccctggt cagtcagatt ttgtctggac
    19201 aacgatccct gacgatatcc cacattaagg cactttcagc ccgatttggc gttaagccgg
    19261 aatggtttct ttgattttga ctgatgccaa accccagatt gcccgatgcg ctgcgcttat
    19321 cgggccttcc tggttcgtgc gacatgtagg ccggataagg cgttcacgcc gcatccggca
    19381 ctgttaccta ctctaaatct tccccattac tggcaatcac ttttttatac caccagaacg
    19441 atttcttacg tgtgcgcgtc agcgtgccgt tgcctgcgtc gtcacggtct acgtagacaa
    19501 aaccgtagcg tttgctcatt tcacccgtag aggcggagac taaatcaata cagccccagg
    19561 tggtgtagcc catcagcgga atgccatctg caatcgcttc gcccattgcg cggatatgtt
    19621 cgcgtaaata gctgatgcga tagtcatcgt taatctcgcc attggcagca agttcatctt
    19681 ttgcgccgag gccgttttcc accagaaaca gcggcttctg ataacggtcg tacatcatgt
    19741 tcatggtgat acgcagaccg agtggatcaa taccccagcc ccagtcgctc acttgcagat
    19801 acgggttacg cagcgatttc actacgttcg ccgcactgct attgttggcg ttcatctccg
    19861 ccgaggcgca gcgcgaggcg taatagctga aagagacaaa atcgacggtg tttttcagga
    19921 tttcatcatc gcccggtgct ttgtcgatgg ttaccccttt ttcgcggaat acgcgggcag
    19981 agtaagccgg atacgtgccc cgcgcctgca catcgataaa aaacaggttt tcccggtctt
    20041 tctccagcgc cgcccagaca tcttccggct tgcaactgta agggtagaag ttaccgcccg
    20101 ccagcataca gcccacctga ttttgcgggt taacctcatg ggcgattttg gtggctagcg
    20161 cactggcaac cagctggtga tgcgcggcct gatatttcac ctgatcctga ttttcacctt
    20221 cttcaaacac cagacccgcg ccggagaacg ggctatgcaa cataatgttg atttcattga
    20281 aggtaagcca gtatttcacc agaccatcaa atgcttcaaa gcaggtccgg gcgtagcggc
    20341 tgaaaaactc caccagcttg cggttacgcc aggagccata ttcggtgacc agatgcatcg
    20401 gcacatcgaa gtggcacaac gtgaccagcg gttcgatacc gtacttttta cactcttcaa
    20461 agacagaacg ataaaaagca atgccctgct gattgggcgt gatttcatcg ccctgcggaa
    20521 agagacggct ccaggcaatt gaggtacgga aaaccttgaa tcccatctct gccatcaggg
    20581 cgatatcttc tttataacga tgataaaaat ccgtcgcctc atggctggga taaaactcgt
    20641 catcgcgcaa ctgaaaacgt ttttccagcc ccagtttcac cgccattcga tgctcgccgt
    20701 gtgggatcat atcgacagtg gtcagccctt tgccaccttc acggaacgca ccttcagact
    20761 ggttggcggc aagcgcgccg ccccataaaa aaccttctgg aaatactgac attttcatcc
    20821 tcaattaaga cttacttctt tggcgacggt cggttgtacg ctctgatgct ttcgcgcctc
    20881 ggcagccgct tcctcaacag gaatatcctc aaagccgagc aacagtgtga ggataaacga
    20941 cagcaccacc gccagcgcca tgaccgcgaa cacccagacg atgctcattg gattcgccgg
    21001 atcgaagaat tgcacgctgg taaataaccc tggcgctgcc attgagtggc tggcaagccc
    21061 cgccataccg gcaaccgcgc cgcaaataaa accgctgata agactggcga taagcggacg
    21121 tttcaggcgg atcgccacgc cgtataacgc cggttcggaa atccccgcca taatggctga
    21181 tgccgccgca gccagcgccg tctggcgcag ttccgggttt ttcgttttcc acgccaccgc
    21241 cagtgatgaa ccgcccagcg acaggttagc gccgatctct gacggcatga ccatcccttc
    21301 tttgccggtt tcggcaatgg tctgaatgat ggttggcgta aagacgcggt gcatcccggt
    21361 cattaccagc agaggccaca gcgcgcccat aatggcgact gaaagccagc ccagataacc
    21421 atgaatggtg taaaccagcg ccgaaatggc gctaccgatc cagataccaa tcgggccaat
    21481 cagcaggatt gccagcggtg cggcaatcaa cacaatcagc attggcttga ggaagttttt
    21541 ggtcaccgcc ggtgtaatgc gatccaccca gcgttcgata tatgacaggc accaggtcat
    21601 caccagcgcc gggatcactg tgtaggtgta tttcaccgcg gtgaccggaa tcagggcaaa
    21661 ttcgacatgt tcaccctggg ccgctttcgc catcagttca ataaaactcg gatgtaccag
    21721 cacacccgcg atggcaatcg ccagcgacat attggtttta aatttgatgg cggcagatgc
    21781 cgcgaccatc agcggcagga agaagaaagc accgtcacca atcacattca gaatggttaa
    21841 ggtcggcgat ccttttgtca gcacgccgct catctcgagg atcatcgcca gcagtttgac
    21901 catcgatccg ccgataatcg ccgggatcag cggtgacatg gtgccgatca gcgcatcgag
    21961 gatccccgca ccaatgcgac gcagcgtgag tttgggttta cccacgggct gtgcgggctg
    22021 catatctccc ggcagcaggc tgacgacttc ctgaaaggct tgtgaaacgg tattgccgat
    22081 aatcacctga cactggttgt cactacgtac cacgccgagc acgccgggga tggtttttaa
    22141 cgtcgggctg tcgataagct ggtcatcttt gataacaacg cgcaaacgcg tcatacagtg
    22201 cgtgaccgcc gagatgttat caacgccgcc cagtgccgct atcaccgagc gtgccagcgc
    22261 cgcataattt ttggccatcg gattttatcc tgttatcagt agggtagatg tagttgttat
    22321 cagtcgacgt aacggaaaaa tgccgatgct gaaacggaat taggaaaccg gtttcacaaa
    22381 atcatgaata gttgtcacta aataagcaag agagttaatt gttcattttt tatatttgtg
    22441 aaaccggtca cctgaatatt tttcccgccg attgcgcaag gacaatcggc aaacggtgca
    22501 gtacactgcg ttgattgcgt gtaaagggat gaaaatgatg acgacgatgc tggaagtggc
    22561 gaagcgcgcc ggggtttcaa aagcgaccgt ttcccgcgtg ctttcaggta atggctacgt
    22621 cagccaggag actaaagatc gcgtgtttca ggcggtagaa gagagcggtt accgcccaaa
    22681 cttgctggcg cgcaatctgt cggcgaagag tactcagacg ctggggttgg tagtgaccaa
    22741 cacgctttac cacggcattt attttagtga attactcttt catgccgcgc gaatggcgga
    22801 agagaaaggg cggcagttgc tattggcaga tggtaaacac agcgccgaag aagagcgcca
    22861 ggcgattcag tatctgctgg atttgcgctg cgacgccatt atgatttacc cgcgtttttt
    22921 aagcgtggat gagatcgatg acatcattga cgcgcacagt cagccgataa tggtgcttaa
    22981 tcgccgcctg cgcaaaaaca gcagccatag cgtctggtgc gatcataaac agaccagctt
    23041 taatgccgtg gcagagttga taaacgccgg acatcaggag attgctttcc ttaccggctc
    23101 gatggattcc cccaccagca ttgaacgtct tgctgggtat aaagacgcgc tggcgcagca
    23161 tggtattgcg ctcaatgaaa aacttatcgc taacggtaaa tggacgcccg ccagcggggc
    23221 cgaaggggta gaaacgttgc tcgaacgtgg ggctaaattt agcgcgttag ttgccagtaa
    23281 cgacgatatg gcgataggtg cgatgaaagc gttgcacgag cgcggcgtag cggtgccaga
    23341 gcaggtgtca gttatcggat tcgatgatat cgcaattgcc ccctatatcg ttccggcgct
    23401 ctccagcgta aaaattccgg tgactgagat gattcaggaa attattggac ggctgatttt
    23461 tatgctcgat ggcggggatt tctcaccgcc gaaaaccttc agcggaaaac tgatccgccg
    23521 cgactcactc attgctcttt cgcgataaac attgtcatca catgtcatcg tcactagtgt
    23581 cgatgacatc ttttttcctg cattacagaa attaatttcc ttaaataaca gtgaattaaa
    23641 actggcatga tttgtgaatg tatcggcgca ttaactgtca ttgctggaga atttgatgaa
    23701 ccgtttcatc attgctgacg cgagtaaatg tattggttgc tgtacctgtg aagtggcgtg
    23761 cgtggtttct catcaggaaa atcaggactg tgcatcgctg accccggaaa catttttacc
    23821 gcgtatccat gtcattaaag gtgtgaacat ttccacggcg acagtttgcc gtcagtgtga
    23881 agatgcaccg tgcgctaacg tctgcccgaa tggtgctatc agccgtgata aagggtttgt
    23941 tcatgtgatg caggaacgtt gcattggttg caaaacctgc gttgtggctt gcccgtatgg
    24001 cgcgatggaa gtggtggtac gtccggtgat tcgcaacagc ggcgcggggc tgaatgtacg
    24061 ggctgacaaa gccgaagcca ataaatgtga cctgtgcaac catcgtgaag acggcccggc
    24121 gtgtatggcg gcttgcccga ctcatgcgct gatttgtgtc gatcgtaata aacttgaaca
    24181 actgagtgca gaaaaacgcc gccgtacggc gctgatgttc taaaaatctt acagaactgt
    24241 tctggcatca aaggaattcc cggaaatatt cgtagaatac ggagctggcg gtatcgccga
    24301 cctgagactg ccggatacga cgcagtcgtg tccggcagga cgggttacgc aattcatgat
    24361 ggcgggaata actcaatggc aaaaaacaca tcttgcggtg tccaactgcg tattcgtggc
    24421 aaagtgcagg gcgtcggttt tcgtccgttt gtctggcagc tggcacagca attaaatctt
    24481 cacggcgatg tctgtaatga cggcgatggc gtagaagtcc ggctgcggga agacccggaa
    24541 acgttacttg ttcaattgca tcagcactgc ccgccgctgg cgcgtattga tagcgtcgag
    24601 cgtgagccgt acatctggtc acaactgccc actgagttca ccatccgcca gagcgcggga
    24661 ggtgccatga atacgcaaat tgtcccggat gccgctactt gccctgcttg ccttgccgaa
    24721 atgaataccc caggcgaacg gcgttatcgt tatccgttta tcaactgtac ccactgcggc
    24781 ccgcgtttca ccattattcg cgccatgcct tacgaccgcc cgtttaccgt gatggcggcg
    24841 tttccgttgt gtccggcttg tgacaaagag taccgtgacc cgctcgatcg tcgcttccac
    24901 gcccagccgg tggcctgccc ggagtgtggc ccacatcttg aatgggtaag tcatggtgaa
    24961 catgcagaac aagaggcggc attacaggcg gctatcgcac agttaaaaat gggcaacatt
    25021 gtcgccatca aagggattgg cggatttcat cttgcctgcg atgcacgtaa cagtaacgcg
    25081 gtggcgacac ttcgggcgcg caaacatcgc ccggcgaaac cgctggcggt catgttgcca
    25141 gtggctgacg gtttaccaga cgctgcgcgc cagttgctta ccacgcccgc cgcgccgatt
    25201 gtgctggtgg ataaaaaata cgttcctgag ctttgtgatg atatcgcccc tgaccttaac
    25261 gaagtcgggg taatgttgcc tgcgaacccg ctccagcatt tgctgttaca ggaactgcaa
    25321 tgcccgctgg tgatgacctc cggcaacctg agcggtaaac caccagctat cagcaacgaa
    25381 caggcgctgg cggatttgca gggcattgcc gacggattct tgatacataa ccgcgacatc
    25441 gtgcagcgga tggatgattc ggtggtgcgc gaaagcggcg aaatgctgcg ccgttcgcgg
    25501 gggtatgtgc cggatgcgct ggctttgcct ccgggcttta aaaatgttcc gcctgtgctg
    25561 tgtctcggcg cggatctgaa aaataccttc tgcctggtgc gcggtgaaca agcggtgttg
    25621 agtcagcatc tgggcgattt aagtgacgat ggcatccaga tgcagtggcg cgaagcgtta
    25681 cgcctgatgc aaaacatcta cgattttacc ccgcaatacg ttgtgcatga cgcgcatccg
    25741 ggctatgtct ccagccagtg ggcgcgtgaa atgaatctgc cgacgcaaac ggtactgcat
    25801 catcatgccc atgcagcggc gtgtctggca gagcatcagt ggccgttgga tggcggtgat
    25861 gtcattgctt tgacgctcga cggtatcggt atgggggaga acggcgcttt gtggggcggc
    25921 gagtgcctgc gggtgaacta tcgcgaatgt gagcacctgg gcggcttgcc tgcagtggcg
    25981 cttccgggtg gcgatttggc agcgaagcag ccgtggcgaa acctgctggc gcagtgcctg
    26041 cgctttgtgc cggagtggca gaattaccct gaaacagcaa gtgtgcaaca gcaaaactgg
    26101 agcgtgctgg cgcgggccat tgagcgtgga attaacgcgc cgctggcgtc atcgtgtggg
    26161 cggttgttcg atgcagtggc ggcggcactg ggctgtgcgc cagccacgtt aagttatgaa
    26221 ggtgaagcgg cttgtgctct ggaggcgctc gcagcctcat gccacggagt gacgcatccg
    26281 gtgacgatgc cgctggtgga caatcaactg gatctcgccg ctttctggca gcagtggctg
    26341 aactggcagg caccggttaa tcaacgcgcg tgggcgtttc atgatgcgct ggcgcagggt
    26401 tttgccgcgt tgatgcgtga gcaggccacg atgcgtggta tcactacgct ggtatttagc
    26461 ggcggggtta ttcataaccg tttgctgcgt gcacgtctgg cgcattatct cgctgatttc
    26521 acattgctct ttccacagag tttaccggcg ggtgatggcg gtttgtctct ggggcagggg
    26581 gttattgctg cggcgcgttg gttagcgggt gaagtccaga acggataaaa cgtgttgcat
    26641 ttggacacca ttgccggatg cggcgtaaac gccttatctg gcctacacat gcgtccccgt
    26701 aggtcggata agacgcgcca gcgtcgcatc cgacatttta ggcacagtag cccacctaca
    26761 tcggcaatgt tttcaacaat ccaaatgcct ctttcatccg atcctcactg accacaaagg
    26821 cgcgaagctg gtcagcatcg tcaacaccgc gagccaccat tccctggcgt tcggtattaa
    26881 tttgccagcg taaatcctga cgctgggttt cgcctgccag atgcagcggt aattccggcg
    26941 ttttgatttt caccagcatc gccggtagtt tcagcggcgt gttattgccg agaagatttt
    27001 ttgccagcac catcgcgcta agttgaatcg gctgcaggaa tggcagtacc tgaccgttga
    27061 tttccgcgca atcgcccagc gcataaatat cgtcattact ggtttgcaga taactatcga
    27121 cgcaaacgcc gcgattaatc gtcagcccgg cgcgtcgtgc cagggctgtt tccgggcgca
    27181 gtccggtggc ggcaattacc gcatccactt cgatactacg ctggtggtcc agcgttgcca
    27241 gaatgccaga atccgttttt tccagcccct gcaactgaga tttcaacagc agatgaacgc
    27301 ccatctccgt caaccgatgc tgcaagcggc tgcttacttc tggtggcatt aatgacgcca
    27361 gaatactggc agcgttgtcg attagcgtga ccgctttgcc tgcacgacaa aaatccatcg
    27421 ccagttcgct accaatcaaa ccaccgccga caatcaacac gcgtcgggca tcccgcagtt
    27481 gcgtttcaca ggcgcgatac tcttgctgac tatttaacgt cagcattaac tcacgcccag
    27541 gcactggcgg gacaaaggca ctggcaccag ttgccagcac cagcttgtcg tattgccact
    27601 gattattctg gcttttcacc acatgggctt cggcatcgat atccgtcacc caggtgtgcg
    27661 gaaacaggtg cagattaaac tgcccggcaa attcacccgc cgtctggcgg gtaaggtcgt
    27721 cggcacgttg cccctggctg ataacatggc tgaggtccgg tttgttgtac tcatccatgc
    27781 tgtcggcggc gatcagggtt aatggaatac tggcgtcctg tttgcgaata tttttcacca
    27841 gttggcgggc ggcgaagccc gaaccaatga tcacaatgcc gttgctcatt ttgcctccga
    27901 tgccagttcg tcaaagacgt ctttgccgag agagcattcc gggcagagga agttatccgg
    27961 aacttcactc cacggcgttc ctggcgcaac gtcctgcatt ggctcgcctt ttgccggatc
    28021 gtaaatccac tggcagacgc tgcactgcat ccgtgggccg aggtcggact tcgtggtggc
    28081 agaggtttct tctttaacca ccgtattcac cgtgctctgc ggcagcggcg cgagcgccca
    28141 ctgacgggcg atttcgcgac catgttcacg gcataactcc agagcgtcct ggtctggtcg
    28201 ccatttggct ttcaggctaa gcgacatttc gaaacccgca tcctgcaggc gcgtggaaag
    28261 acgatccacc gcaccgccgc tccagccgtg agagccgaaa gcactggcgc gtttgttacg
    28321 gaagcgtaat ccggtcatct cctccaccag accggcgatt ttcggcatca tcacgttatt
    28381 catcgtcgaa gtgccgacca gcacgccttt tgagcggaag acattggtca ggatttcgtt
    28441 tttatcgctt cgggcgacgt tgaaaatttt caccgccacg cgtgggtcgg tttccgcaat
    28501 cccctgggcg atagcgtcag ccatcatgcg ggtgttattc gacatggtgt cgtagaaaat
    28561 ggtgattctg tcttcctgat aatcagccgc ccatttcagg tacagctcga caatttgcgt
    28621 cgggttatcg cgccatacca cgccgtgaga ggtcgctatc atatcgactg gtaagttaaa
    28681 gcccaggatc tcggtaattt tcggtgttac caggcggctg aacggcgtca ggatattggc
    28741 gtagtaacgc tggcactgct cgaaaagctc cgtctgatcc acttcatcgt tgaacagatg
    28801 ctcgtcgcag tagtgctgac cgaaagcatc gttactgaac agcaccgcgt cgcctgtcag
    28861 gtaagtcatc atgctgtccg gccagtgcag cattggcgtt tcgacaaaaa tgagctgttt
    28921 gccgttgccg atatccagcg tgtcaccagt tttcaccaca ttaaaattcc actccggatg
    28981 atgatgatga ccatttatcg agtcgatagc gttggcagta cagtaaatcg gcgtatcggg
    29041 aatttgcgcc atcagttcgg tcagcgcccc agcgtggtcc tcttctgcat ggttaatcac
    29101 gatgtaatcg atatccgcca gatcgatttc attacgcagg ttctgcacaa attcgcggct
    29161 gaatttatgg tcgacggtgt cgatcagcac gtttttttct tcgcggatga ggtagctgtt
    29221 gtaactgctg ccgcgtagcg ttttatattc cgtaccgtga aaatcacgca cttcccagtc
    29281 acgttgacca acccaatgaa tgttattttt caccacaata gacatagcaa cctcaattta
    29341 ttcagcgtgt tctaaaaaga tgtcttgcta attgcagatt acgtgccagt tttttatctg
    29401 attgttttta ctctataaat aaaattatgc tcattaatga gtagtcaaaa tgactatcaa
    29461 tcaaatggtc gatatgacaa tatctatagt caaattgaca gtgaggcaaa gatgagtttt
    29521 tccgttgatg tgctggcgaa tatcgccatc gaattgcagc gtgggattgg tcatcaggat
    29581 cgttttcagc gcctgatcac cacgctacgt caggtgctgg agtgcgatgc gtctgcgttg
    29641 ctacgttacg attcgcggca gtttattccg cttgccatcg acggtctggc aaaggatgta
    29701 ctcggtagac gctttgcgct ggaagggcat ccacggctgg aagcgattgc ccgcgccggg
    29761 gatgtggtgc gctttcccgc agacagcgaa ttgcccgatc cctatgacgg tttgattcct
    29821 gggcaggaga gtctgaaggt tcacgcctgc gttggtctgc cattgtttgc cgggcaaaac
    29881 ctgatcggcg cactgacgct cgacgggatg cagcccgatc agttcgatgt tttcagcgac
    29941 gaagagctac ggctgattgc tgcgctggcg gcgggagcgt taagcaatgc gttgctgatt
    30001 gaacaactgg aaagccagaa tatgatgcca ggcgatgcca cgccgtttga agcggtgaaa
    30061 cagacgcaga tgattggctt gtcccctggc atgacgcaac tgaaaaaaga gattgagatt
    30121 gtggcggcgt ccgatctcaa cgtcctgatc agcggtgaga cgggaaccgg taaggagctg
    30181 gtggcgaaag cgattcatga agcctcgcca cgggcggtga atccgctggt ctatctcaac
    30241 tgtgctgcac tgccggaaag tgtggcggaa agtgagttgt tcgggcatgt gaaaggagcg
    30301 tttactggcg ctatcagtaa ccgcagcggg aagtttgaaa tggcggataa cggcactctg
    30361 tttctggatg agatcggcga gttgtcgttg gcattgcagg ccaagctgct gagggtgttg
    30421 cagtatggcg atattcagcg cgttggcgat gaccgtagtt tgcgggtcga tgtgcgcgtg
    30481 ctggcggcga ctaaccgcga cttacgcgaa gaggtgatgg cagggcgatt tcgcgctgac
    30541 ttgtttcatc gcctgagcgt gtttccactt tcggtgccgc cgctgcgtga gcggggcgat
    30601 gatgtcattc tgctggcggg gtatttctgc gagcagtgtc gtttgcggct ggggctctcc
    30661 cgcgtggtat taagtgccgg agcgcgaaat ttactgcaac actatcgttt tccggggaac
    30721 gtgcgcgaac tggaacatgc tattcatcgg gcggtagtgc tggcgagagc cacccgcaac
    30781 ggcgatgaag tgattcttga ggcgcaacat tttgcttttc ctgaggtgac gttgccgccg
    30841 ccagaagcgg cggcggtgcc cgttgttaag caaaacctgc gtgaagcgac agaagcgttc
    30901 cagcgtgaaa ctattcgcca ggcactggca caaaatcatc ataactgggc tgcctgcgcg
    30961 cggatgctgg aaaccgacgt cgccaacctg catcggctgg cgaaacgtct gggaatgaag
    31021 gattaaataa tcccggcctg atagaaatcc tgcaggttaa ttgcgccggt gagtttgccg
    31081 ttttcatcca ccaccggtgc ggcagtgatt ttgcgcttca tcaggatctc tttggcgtcg
    31141 atggcgcgac tttgcgcctg taacgtggtg ccgcctgtcg tcatcgcttc attaactggc
    31201 gtggtgagtg cgccgccgcc taccagccag cgacgtaaat cgccgtcggt aaagacgcct
    31261 tgtacctgtt gttgagcgtc acataccgcc accagcccca gaccggtgcg gctgagttcc
    31321 agcatcgcat ccatcacgct ggcggttaac gccacctgtg ggatggcatc gtcacggcgc
    31381 atcagatgat gcactttatt cagcaagcga gcgcccagtg ccccggctgg gtgggagcgg
    31441 gcaaaatctt cttcattaaa tccgcgcgcc tgcatgaccg ccatcgccag cgcgtcaccc
    31501 atcatcaggg tattgacggt gctggaggtc ggcgcaaggt gcatcgggca ggcttcgcgt
    31561 tctacggaga tatccagcac cgcttttgcc gccaggccca gcggtgacgt cggtttgccg
    31621 gtcatcgcca gcagcgcgat agatttatct tccagacgcg gaataatcag atccagttcc
    31681 ttcgcgccac cggagtaaga gataaacagc atcacatcgc ggctttcaat catccccaga
    31741 tcgccgtgca gtgcttctgc cggatggaca aaaaaagccg atgtgccggt actggcaagt
    31801 gttgcggcga ttttcttacc aatgtggccc gatttgccaa ttcccgaaac caccactttg
    31861 ccttcgcagt gcaggatgat attggcggcg cgaacaaaat catcgcccag acgttccggt
    31921 aaacggcttg cttcctgcaa ctccagcatt aacgtctgac gtcccgcgtt cagtagtgct
    31981 tcactcattg ctctctccgg ttatgatcac atcgatccct ttctcttcca gcgcctgacg
    32041 aaacgccgga tcgatacctg cgtcggtaat cagcttatcg acgctttcaa gactgcaaac
    32101 tacgttgggg cttttacggc caaactttga tgagtccgcc atcaaaatca cttcgcgcgc
    32161 ggcattgcac attgccttac tgacggtata aacctcgtta aaggtggtta cgcccgcatt
    32221 gagatcgatg ccgtcggtgc ccataaacaa tttatcgaaa ctgaaatgtt cgaaggcatt
    32281 ctctgccagc tgtccgtgaa atgaggccga ttttttacga aacgttccgc ctggcatcag
    32341 gatagtttgt tcgttatcca gttcagatag cgcattgacg atatgcagac tgttggtcat
    32401 caccgtgatg ttattaaagc gcgagagcag gggaaccatc tgcaaaacgg tactgccagc
    32461 atcaagaatg atcgaatcgc catcatgaat aaaactaacg gcagcttctg ctatcagctc
    32521 tttcttgtgg gtgttgatga gtgttttatg atcgataggc ggatcggatt cctctttatt
    32581 caacaccact ccgccataag tacgaatgac ggttcctgca tgttccagaa tgaccagatc
    32641 tttgcgaatg gttgtgcctg tggtgtcaaa gtattgcgcc aattcttcaa ccgagcattt
    32701 accctgcttt tgcagatact ccagaatggc ggcctgacgc tgacgaggtt tcatgggcgt
    32761 gattacctga tttaggtttc aaaatgataa cttcgcaagc aagtaacatt cacaacgaaa
    32821 ttatccacgt ttcagtttaa gcgccaatga tagagcattc tgtgatagtg gatcatgggg
    32881 aaagatcaca tcgggctgta aatcacccgc atgcataccg gtaattgcgg gaattttttg
    32941 cggtcgtgcg aagacggtca gtcctttcat aaacaaggtg tcgacgatgc gctgctgatc
    33001 gtccagggcg atggcgacca cgacccgtgg tttaaagcgc ccgctggaac ggcccacgcc
    33061 aacccgccct tgctggcata gtgtgtcgaa ggcacggtta aaacgagaaa tttgccagcc
    33121 gcctaaggcc agttgcgcac accaggcgat aacggcgacg gtgatgagtg cggataccat
    33181 aacttttcct taatgtagtg gcggaagtcc tgtatgcctg atgagcgtag cgcatcaggc
    33241 attctattag tctcacggag atccttaacg gatctccgct gttgattaca acatcacctg
    33301 accgccggtg acattgatcg actgtccggt gcagtacgac gccttaggac tggcatagag
    33361 cagcagcata ttcagcacat cctgataatc gcagccgcgt ttgagcggta ctttgtcgat
    33421 gtaatactgc tcgacttgat ccggtttgat acccagcttg gtcgcgtatt ggggtaacag
    33481 tgactggaac ataggcgatt tcagcaggtt accgagcatc agtgaatgca ccgtaatgcc
    33541 gtactccgcc agatccagcg ccagtgattg agtcagcccg acgccaccaa atttcgctgc
    33601 gctgtagcca gagttgtgtt tgctgcccac tttgccggat ttcgagttga tctgaataat
    33661 gcgaccctga atcccgtcgc ggatcatcaa acgagaaaat tcacgggcac acaggaaata
    33721 acccaccaga ttcacggtca aaatcgccga gctggaagtc gctgataaag gctgcttttg
    33781 ctattccggc gctgtagacc agcaaatcca cgcgcccaaa gatttcatct accccacgag
    33841 agagcgccag gacgctttgc tcgctggtgg cgtcagcacc aaaactatac gctgttcctt
    33901 caccatattc ggcgttaatt tcttgtgcca catttgcggc tttgtcgctc tgaatatcga
    33961 caaccgcgac gcgatacccc tcggcagcca gaccgtggca caggaacgcg cctaaggttt
    34021 gcccaccacc gatgacaacg gcaacctgat tcatttttta ctccttaaca gattcaaact
    34081 tcaaaaccga tcccggcgcg atatcgtcgg gtacagggcc tgccacatgg acagtgcccg
    34141 gaaattccgc ttcgtttaaa ccatcgaagc gcagggtgac atgacccagt tcgcgaaggt
    34201 tgtcttccgc cacgctgcca acagcggtca ccggatagcg atgctgcccg agcgtaaatt
    34261 gcaaaccggg atggagtgca cctttcaact cgccgtggca atgaatgaag caatactctt
    34321 cgaggtccgc tggcgcgcct tcacgaaagg tgatgagcat ctggtcgctg agggcgtcag
    34381 tggcactcgc gccgatacgg gtgatggtgg tctgataaat aacggtcatg tttcagcctc
    34441 tattgataga taaaaccgga gacaaaccag gcgatcagta cagtcggtgc gccggttaaa
    34501 aagtggctca ccagtacaga agggacaccg acgcgaaccg tgtcctgacg ggcttccgcc
    34561 agcgacaaac cgaccgggat gaagtcgcag gccgcctgcg cgttgatggc aaacagtgcc
    34621 ggtaaagcca gatgcggcgg aatattgccg agaccaatct gcacgccaat caatacgccg
    34681 ataacctgtg cgataactgc gcctgggccg aggaaaggtg aaagcagcgg gaaggagcag
    34741 atgagcgcca gcatgaccag acctagtgga tggctcgcca gcggagcaag accgtgggca
    34801 atccagtcac caaggccaga agccataatg atgccaatga gcgccgagac gaacgccata
    34861 aacggcagaa tggtttttaa tacagtgtcg atggtgtcac gaccagattg aaacaacacg
    34921 gcaacggcag aacccatacc cattcccacc ttcgccagta aaccatcgct ttgttcggtg
    34981 attttcttgc tggtgtcata gtcacggccc acggaagagg gttgtggtgt cgcatcaccc
    35041 actacagtga tgttttcttc ttttacgcca gagacataaa tatcttccac aatgtactgc
    35101 gccagcggac tggacttgcc cgtcgagtgg atattaatgg tgggaatacg tcgtttcgga
    35161 tagatgccgc agcgtaatgt gccgccacag tcgattaccg cgacaccaat ttccgcctcc
    35221 gcgggttcac cttctttaaa tccgtcaata gcctgccagc cagtaagttg tgccagtttg
    35281 tcaacaatcg caggccgggt accggcggtg atatagacga tttttttgcc cggcgtggct
    35341 tccagctcaa gcgggccgcc ccagccaccc gttccttttt cgatccgaat acgcgtcatg
    35401 atgttgctcc tgccaggtga actttttgtt caagttgaat gcccatcttt ttctcaaaaa
    35461 tggcggtggt cagatcggtc acccagccgc gaaagaagtt ggtgaccaga ccaaccagca
    35521 gatagctcac cgccagtggg ccaagtggca ggttcagcgt tgtcagaccg ctggcaatgc
    35581 caagataaac aaacagttcg ccagggttga tatggggaaa gaggccattc attgagtggc
    35641 agctataaga ggctgccgcg tagtagctgg gtttgtactt ttccggcata aagcgaccca
    35701 ggcttaaggt catcggattg caaaagacaa acgtgccaat gcacggtagc agcaggtaac
    35761 gggaaacagg gttaccggcg caacgttgag caaaacgttc aatacgatgc tgaccgataa
    35821 aattaatcag tgcgttcatg ataaccagca ggctaatcaa cagcggaaga atgccggtca
    35881 ccatcccggt aaacacctct ccgccctttt ggaacagccc gataaaccac tctgcaccat
    35941 gagtaatggt ttctatcatt gttctctcct tcaggattta ttgttttatt accaaacggc
    36001 aacctaatct aatcagattg aaagatttga aagtgttatt ttgatcgcaa aatgaaagat
    36061 aaatatttta atttgaaagt ttgaataaaa ggatagcgag gggaatgagt tgagttatgt
    36121 aaagtccgta tcgggcagtg actaccgctt ccttgtgcgg ggcgtgatgc tttaccatac
    36181 ttgcccctgg ttgaatctgt taaatggacc cctcatgttc aagcgtcgtt atgtaacatt
    36241 gcttcccctt tttgtgttgc ttgccgcctg tagcagcaag ccaaaaccta ctgagactga
    36301 aacgaccacc ggaacgccgt ctggcggctt cctgcttgag cctcagcaca atgtgatgca
    36361 gatgggcggc gatttcgcta ataacccgaa tgcccagcag ttcatcgaca gaatggtgaa
    36421 caaacacggt ttcgatcgtc agcagttgca ggaaattctc tcccaggcga agcgtctgga
    36481 ttcggtactg cggctgatgg ataaccaggc accaaccaca tcggtgaaac cgccatcagg
    36541 tccgaacggc gcatggctgc gttatcgcaa aaaatttatt acgccggaca acgtgcagaa
    36601 cggtgtggtt ttctggaatc agtatgaaga tgcgttgaat cgcgcgtggc aggtgtatgg
    36661 cgtaccgccg gaaattatcg tcgggattat cggcgttgaa acccgctggg ggcgcgtgat
    36721 ggggaaaact cgcatcctcg atgcgctggc aacgctgtca tttaactacc cacgccgcgc
    36781 ggagtatttc tctggcgagc tggaaacctt cctgttgatg gcgcgcgacg agcaggacga
    36841 tccgctcaat ctgaaaggtt cctttgccgg ggcgatgggc tacggacagt ttatgccgtc
    36901 gtcttacaaa caatatgcgg tagatttcag cggcgacggg catatcaacc tgtgggatcc
    36961 ggttgatgcg atcggtagtg tggcgaacta tttcaaagcg cacggctggg tgaaaggcga
    37021 tcaggtcgcg gtaatggcaa acggtcaggc tccaggcttg ccaaatggct tcaaaaccag
    37081 gtacagcatt tcgcagcttg ccacggcagg tttaacgcca cagcagccgc tgggcaacca
    37141 tcaacaagcc agcctgctgc gtctggatgt cggtaccggt taccagtact ggtacggtct
    37201 gccgaacttc tacaccatta cccgttataa ccacagcacc cattacgcaa tggcggtctg
    37261 gcagttagga caagccgtgg cgctggcgcg cgtgcagtaa ctatttgcaa gaggggggcg
    37321 tgagcttccc tctggctaat cagctttttt ctgaatcctc ctcgtaaaat tgcaacgcca
    37381 acaccatctt cctgacgaaa gtgctatctt gtccggcata aatttttgac tgactgaggt
    37441 tgtgatgact gacagtgaac tgatgcagtt aagcgaacag gttgggcagg cgctgaaagc
    37501 ccgtggcgca accgtaacaa ctgccgagtc ttgtaccggt ggttgggtag cgaaagtgat
    37561 taccgatatt gccggtagct ccgcctggtt tgaacgcgga tttgtcacct acagtaacga
    37621 agccaaagcg cagatgatcg gcgtacgcga agagacgctg gcgcagcatg gcgcggtgag
    37681 tgaacccgtc gtggtggaaa tggcgatagg tgcactgaaa gcggctcgtg ctgattatgc
    37741 cgtgtctatt agtggtatcg ccgggccgga tggcggcagt gaagagaagc ctgtcggcac
    37801 cgtctggttt gcttttgcca ctgcccgcgg tgaaggcatt acccggcggg aatgcttcag
    37861 cggcgaccgt gatgcggtgc gtcgtcaggc tactgcgtat gcattgcaga ccttgtggca
    37921 acaatttcta caaaacactt gatactgtat gagtatacag tataattgct tcaacagaac
    37981 atattgacta tccggtatta cccggcatga caggagtaaa aatggctatc gacgaaaaca
    38041 aacagaaagc gttggcggca gcactgggcc agattgagaa acaatttggt aaaggctcca
    38101 tcatgcgcct gggtgaagac cgttccatgg atgtggaaac catctctacc ggttcgcttt
    38161 cactggatat cgcgcttggg gcaggtggtc tgccgatggg ccgtatcgtc gaaatctacg
    38221 gaccggaatc ttccggtaaa accacgctga cgctgcaggt gatcgccgca gcgcagcgtg
    38281 aaggtaaaac ctgtgcgttt atcgatgctg aacacgcgct ggacccaatc tacgcacgta
    38341 aactgggcgt cgatatcgat aacctgctgt gctcccagcc ggacaccggc gagcaggcac
    38401 tggaaatctg tgacgccctg gcgcgttctg gcgcagtaga cgttatcgtc gttgactccg
    38461 tggcggcact gacgccgaaa gcggaaatcg aaggcgaaat cggcgactct cacatgggcc
    38521 ttgcggcacg tatgatgagc caggcgatgc gtaagctggc gggtaacctg aagcagtcca
    38581 acacgctgct gatcttcatc aaccagatcc gtatgaaaat tggtgtgatg ttcggtaacc
    38641 cggaaaccac taccggtggt aacgcgctga aattctacgc ctctgttcgt ctcgacatcc
    38701 gtcgtatcgg cgcggtgaaa gagggcgaaa acgtggtggg tagcgaaacc cgcgtgaaag
    38761 tggtgaagaa caaaatcgct gcaccgttta aacaggctga atttcagatc ctctacggcg
    38821 aaggtatcaa cttctacggc gaactggttg acctgggcgt aaaagagaag ctgatcgaga
    38881 aagcaggcgc gtggtacagc tacaaaggtg agaagatcgg tcagggtaaa gcgaatgcaa
    38941 ctgcctggct gaaagataac ccggaaaccg cgaaagagat cgagaagaaa gtacgtgagt
    39001 tgctgctgag caacccgaac tcaacgccgg atttctctgt agatgacagc gaaggcgtag
    39061 cagaaactaa cgaagatttt taatcgttcg ttattgatga taatagataa tgtgtgcacg
    39121 gacggtcccc tcgccccttt ggggagaggg ttagggtgag gggaacaggc caacactggc
    39181 gtgaaaaatt actctcacct cgatcctcta acgaattggc tgcacactca ttgactatta
    39241 aatctgttta tcagcaaaac ctgggtcgca tctgcggccc ttttgctttt ttaagttgta
    39301 aggatatgcc atgacagaat caacatcccg tcgcccggca tatgctcgcc tgttggatcg
    39361 tgcggtacgc attctggcgg tgcgcgatca cagtgagcaa gaactgcgac gtaaactcgc
    39421 ggcaccgatt atgggcaaaa atggcccaga agagattgat gctacggcag aagattacga
    39481 gcgcgttatt gcctggtgcc atgaacatgg ctatctcgat gacagccgat ttgttgcgcg
    39541 ctttatcgcc agccgtagcc gcaaaggtta tggacctgca cgtattcgtc aggaactgaa
    39601 tcagaaaggt atttcccgcg aagcgacaga aaaagcgatg cgtgaatgtg acatcgactg
    39661 gtgcgcactg gtgcgcgatc aggcgacgcg aaaatatggt gaacctttgc caactgtctt
    39721 ttcagaaaaa gttaagatcc agcgttttct gctctatcgt ggctatctga tggaagatat
    39781 ccaggatatt tggctaaatt ttgccgactg aacgcatacg gtattttact tcccactcaa
    39841 gaaaacttat cttattccca cttttcagtt accagcccgg cggttaagac acgctggagc
    39901 tggtggcgat atttcgttag cttgatttca ggataattat gagcaagagc accgctgaga
    39961 tccgtcaggc gtttctcgac tttttccata gtaagggaca tcaggtagtt gccagcagct
    40021 ccctggtacc ccataacgac ccaactttgt tgtttaccaa cgccgggatg aaccagttca
    40081 aggatgtgtt ccttggactc gacaagcgta attattcccg cgctaccact tcccaacgct
    40141 gcgtgcgtgc gggtggtaaa cacaacgacc tggaaaatgt cggttacacc gcgcgtcacc
    40201 ataccttctt cgaaatgctg ggcaacttca gcttcggcga ctatttcaaa cacgatgcca
    40261 ttcagtttgc atgggaactg ctgaccagcg aaaaatggtt tgccctgccg aaagagcgtc
    40321 tgtgggttac cgtctatgaa agcgacgacg aagcctacga aatctgggaa aaagaagtag
    40381 ggatcccgcg cgaacgtatt attcgcatcg gcgataacaa aggtgcgcca tacgcatctg
    40441 acaacttctg gcagatgggt gacactggtc cgtgcggccc gtgcaccgaa atcttctacg
    40501 atcacggcga ccacatttgg ggtggccctc cgggaagtcc ggaagaagac ggcgaccgct
    40561 acattgagat ctggaacatc gtcttcatgc agttcaaccg ccaggcagat ggcacgatgg
    40621 aaccgctgcc gaagccgtct gtagataccg gtatgggtct ggagcgtatt gctgcggtgc
    40681 tgcaacacgt taactctaac tatgacatcg acctgttccg cacgttgatc caggcggtag
    40741 cgaaagtcac tggcgcgacc gatctgagca ataaatcgct gcgcgtaatc gctgaccaca
    40801 ttcgttcttg tgcgttcctg atcgcggatg gcgtaatgcc gtccaatgaa aaccgtggtt
    40861 atgtactgcg tcgtatcatt cgtcgcgcag tgcgtcacgg caatatgctc ggcgcgaaag
    40921 aaaccttctt ctacaaactg gttggtccgc tgatcgacgt tatgggctct gcgggtgaag
    40981 acctgaaacg ccagcaggcg caggttgagc aggtgctgaa gactgaagaa gagcagtttg
    41041 cccgtactct ggagcgcggt ctggcgttgc tggatgaaga gctggcaaaa ctttctggtg
    41101 atacgctgga tggtgaaacg gctttccgtc tgtacgacac ctatggcttc ccggttgacc
    41161 tgacggctga tgtttgtcgt gagcgcaaca tcaaagttga cgaagctggt tttgaagcag
    41221 caatggaaga gcagcgtcgt cgcgcgcgcg aagccagcgg ctttggtgcc gattacaacg
    41281 caatgatccg tgttgatagt gcatctgaat ttaaaggcta tgaccatctg gaactgaacg
    41341 gcaaagtgac cgcgctgttt gttgatggta aagcggttga tgccatcaat gcaggccagg
    41401 aagctgtggt cgtgctggat caaacgccat tctatgcgga atccggcggt caggttggtg
    41461 ataaaggcga actgaaaggc gctaacttct ccttcgcggt ggaagatacg cagaaatacg
    41521 gccaggcgat tggtcacatc ggtaaacttg ctgcgggttc tctgaaagtg ggcgacgctg
    41581 tgcaggctga tgttgatgag gctcgtcgcg cccgtattcg tctgaatcac tccgcaacgc
    41641 acctgatgca cgctgcgctg cgccaggttc tggggactca tgtatcgcag aaaggttcac
    41701 tggttaacga caaggtgctg cgcttcgact tctcacacaa cgaagcgatg aaaccggaag
    41761 agattcgtgc ggtcgaagac ctggtgaacg cacagattcg tcgcaatttg ccgatcgaaa
    41821 ccaacatcat ggatctcgaa gcggcgaaag cgaaaggtgc gatggcgctg ttcggcgaga
    41881 agtatgatga gcgtgtacgc gtgctgagca tgggcgattt ctccaccgag ctgtgtggcg
    41941 gtactcacgc cagccgcact ggtgatattg gtctgttccg catcatctct gaatcgggta
    42001 ctgctgcagg cgttcgtcgt atcgaagcgg taaccggaga aggcgctatc accaccgttc
    42061 atgcagacag cgatcgctta agcgaagtcg cgcatctgct gaaaggcgat agcaataatc
    42121 tggcggataa agtgcgctca gtactggaac gtacgcgtca gttagaaaaa gaactacaac
    42181 agcttaaaga acaagctgcc gcacaggaga gcgcaaatct ttccagtaag gcaattgatg
    42241 ttaatggtgt taagctgttg gttagcgagc ttagcggtgt tgagccgaaa atgttgcgta
    42301 ccatggttga cgatttaaaa aatcagctgg ggtcgacaat tatcgtgctg gcaacggtag
    42361 ccgaaggtaa ggtttctctg attgcaggcg tatctaagga cgtcacagat cgtgtgaaag
    42421 caggggaact gattggtatg gtcgctcagc aggtgggcgg caagggtggt ggacgtcctg
    42481 acatggcgca agccggtggt acggatgctg cggccttacc tgcagcgtta gccagtgtga
    42541 aaggctgggt cagcgcgaaa ttgcaataat ataagcgtca ggcaatgccg tggactcgct
    42601 tcacggcatt cgcattaacg ctatcgacaa cgataaagtc aggttgaagt tgtgtatatc
    42661 ggctaaactt aggtttaaca gaatgtaatg ccatgactgc ttacatgtaa tgtgtttgtc
    42721 attgcttact ttttggcgtt atatgatgga taatgccggg atacagagag acccgactct
    42781 tttaatcttt caaggagcaa agaatgctga ttctgactcg tcgagttggt gagaccctca
    42841 tgattgggga tgaggtcacc gtgacagttt taggggtaaa gggcaaccag gtacgtattg
    42901 gcgtaaatgc cccgaaggaa gtttctgttc accgtgaaga gatctaccag cgtatccagg
    42961 ctgaaaaatc ccagcagtcc agttactaat ctttccgcgt ctcatcttta tcggtgagac
    43021 gcaccctcaa aatttctcct tcactctata gtcttttcgc tttactcccg ttcattcaac
    43081 ttaagtctcc atttttttgc attactgcta tctgccagac ctccgttttt ctgtagataa
    43141 aacactcttt ttgacgtttt tacagactaa ttgagcgtga agtgtgcaaa cgataaaagt
    43201 gtgggaaaaa ttgtttgact tataagtctc agaaagtaat atgtgcgcca cgcagcgacg
    43261 atgagcgata aacaagttct tcgaagcact cgtaagaggc gtgtggtgag gtggccgaga
    43321 ggctgaaggc gctcccctgc taagggagta tgcggtcaaa agctgcatcc ggggttcgaa
    43381 tccccgcctc accgccattt gcatccgtag ctcagctgga tagagtactc ggctacgaac
    43441 cgagcggtcg gaggttcgaa tcctcccgga tgcaccatct cttacttgat atggctttag
    43501 tagcggtatc aatatcagca gtaaaataaa ttttcccgat gcatccgtag ctcagctgga
    43561 tagagtactc ggctacgaac cgagcggtcg gaggttcgaa tcctcccgga tgcaccatat
    43621 tctccgaaat cttcagcaat gaaggtatcg aagaggtagc ggaattaacc gcaggcacca
    43681 gggatgataa cgttgcttta gcaacggccc gaagggcgag ccgcaaggcg agtaatcctc
    43741 ccggatgcac catctcttaa ttgatatggc tttagtagcg gtatcaatat cagcagtaga
    43801 ataagttttc ccgatgcatc cgtagctcag ctggatagag tactcggcta cgaaccgagc
    43861 ggtcggaggt tcgaatcctc ccggatgcac catattctcc gtaaccttca gcaatgaagg
    43921 tatcgaagag atagcggtat taaccgcagg caccaggaag gataacgttg cgccagcaac
    43981 ggcccgaagg gcgaacgtca gtgagtcatc ctcccggatg caccatcttc tccaaaaaat
    44041 ttccttcaaa aatatctcat ttctaaataa ctcctttatc catttcctga attttcttct
    44101 ccttcgccat cagcgacaaa atcaagcaat tacgctaaag tagcgtcata tttttcgact
    44161 tgcgagaaga ccatgtacga gcgttatgca ggtttaattt ttgatatgga tggcacaatc
    44221 ctggatacgg agcctacgca ccgtaaagcg tggcgcgaag tattagggca ctacggtctt
    44281 cagtacgata ttcaggcgat gattgcgctt aatggatcgc ccacctggcg tattgctcag
    44341 gcaattattg agctgaatca ggccgatctc gacccgcatg cgttagcgcg tgaaaaaaca
    44401 gaagcagtaa gaagtatgct gctggatagc gtcgaaccgc ttcctcttgt tgatgtggtg
    44461 aaaagttggc atggtcgtcg cccaatggct gtaggaacgg ggagtgaaag cgccatcgct
    44521 gaggcattgc tggcgcacct gggattacgc cattattttg acgccgtcgt cgctgccgat
    44581 cacgtcaaac accataaacc cgcgccagac acatttttgt tgtgcgcgca gcgtatgggc
    44641 gtgcaaccga cgcagtgtgt ggtctttgaa gatgccgatt tcggtattca ggcggcccgt
    44701 gccgcaggta tggacgctgt ggacgttcgc ttgctgtgag tgaagcgtta tcgcttttct
    44761 cgttgtttgc cagtagtttt ctcagcgcta cactattacc cggcaactcc gaagtcgtgc
    44821 tggtggcaat gttgctttcc gggatcagtc atccctgggt tttagtttta acagcaacaa
    44881 tgggtaatag ccttggaggg ttaactaacg ttatccttgg gcgtttcttt ccattgcgta
    44941 aaacatcgcg ctggcaagag aaagctatcg gctggctgaa acgctatggt gcagtcacgc
    45001 tattattaag ctggatgccc gtggttggcg atttactgtg tctgttagcg ggatggatgc
    45061 gcatctcgtg gggaccggta atcttttttt tgtgccttgg taaagcgtta cgctatgttg
    45121 cagttgcagc agcgaccgtt cagggcatga tgtggtggca ctaattgtag gcctgcacat
    45181 atggtcacca ttacagttat gctaattaaa acgattttga caggcgggag gtcaatttga
    45241 tcccggacgt atcacaggcg ctggcctggc tggaaaaaca tcctcaggcg ttaaagggga
    45301 tacagcgtgg gctggagcgc gaaactttgc gtgttaatgc tgatggcaca ctggcaacaa
    45361 caggtcatcc tgaagcatta ggttccgcac tgacgcacaa atggattact accgattttg
    45421 cggaagcatt gctggaattc attacaccag tggatggtga tattgaacat atgctgacct
    45481 ttatgcgcga tctgcatcgt tatacggcgc gcaatatggg cgatgagcgg atgtggccgt
    45541 taagtatgcc atgctacatc gcagaaggtc aggacatcga actggcacag tacggcactt
    45601 ctaacaccgg acgctttaaa acgctgtatc gtgaagggct gaaaaatcgc tacggcgcgc
    45661 tgatgcaaac catttccggc gtgcactaca atttctcttt gccaatggca ttctggcaag
    45721 cgaagtgcgg tgatatctcg ggcgctgatg ccaaagagaa aatttctgcg ggctatttcc
    45781 gcgttatccg caattactat cgtttcggtt gggtcattcc ttatctgttt ggtgcatctc
    45841 cggcgatttg ttcttctttc ctgcaaggaa aaccaacgtc gctgccgttt gagaaaaccg
    45901 agtgcggtat gtattacctg ccgtatgcga cctctcttcg tttgagcgat ctcggctata
    45961 ccaataaatc gcaaagcaat cttggtatta ccttcaacga tctttacgag tacgtagcgg
    46021 gccttaaaca ggcaatcaaa acgccatcgg aagagtacgc gaagattggt attgagaaag
    46081 acggtaagag gctgcaaatc aatagcaacg tgctgcagat tgaaaacgaa ctgtatgcgc
    46141 cgattcgtcc aaaacgcgtt acccgcagcg gcgagtcacc ttctgatgcg ctgttacgtg
    46201 gcggcattga atatattgaa gtgcgttcgc tggacatcaa cccgttctcg ccgattggtg
    46261 tagatgaaca gcaggtgcga ttcctcgacc tgtttatggt ctggtgtgcg ctggctgatg
    46321 caccggaaat gagcagtagc gaacttgcct gtacgcgcgt taactggaac cgggtgatcc
    46381 ttgaaggtcg caaaccgggc ctgacgctgg gtatcggctg cgaaaccgcg cagttcccgt
    46441 tactgcaggt gggtaaagat ctgttccgcg atctgaaacg cgtcgcgcaa acgctggata
    46501 gcattaacgg cggcgacgcg tatcagaaag tgtgtgatga actggttgcc tgcttcgata
    46561 atcccgatct gactttctct gcccgtatct taaggtctat gattgatact ggtattggcg
    46621 gaacaggcaa agcgtttgca gaagcctacc gtaatctgct gcgtgaagag ccgctggaaa
    46681 ttctgcgcga agaggatttt gtagccgagc gcgaggcatc tgaacgccgt cagcaggaaa
    46741 tggaaaccgc tgataccgaa ccgtttgcgg tgtggctgga aaaacacacc tgacagaaaa
    46801 gaaaaaggcc actcgtgagt ggccaaaatt tcatctctga attcagggat gatgataaca
    46861 aatgcgcgtc tttcatatac tcagactcgc ctgggaagaa agagttcaga aaatttttaa
    46921 aaaaattacc ggaggtggct aaatgccgtt gttagatagc ttcacagtcg atcatacccg
    46981 gatggaagcg cctgcagttc gggtggcgaa aacaatgaac accccgcatg gcgacgcaat
    47041 caccgtgttc gatctgcgct tctgcgtgcc gaacaaagaa gtgatgccag aaagagggat
    47101 ccataccctg gagcacctgt ttgctggttt tatgcgtaac catcttaacg gtaatggtgt
    47161 agagattatc gatatctcgc caatgggctg ccgcaccggt ttttatatga gtctgattgg
    47221 tacgccagat gagcagcgtg ttgctgatgc ctggaaagcg gcaatggaag acgtgctgaa
    47281 agtgcaggat cagaatcaga tcccggaact gaacgtctac cagtgtggca cttaccagat
    47341 gcactcgttg caggaagcgc aggatattgc gcgtagcatt ctggaacgtg acgtacgcat
    47401 caacagcaac gaagaactgg cactgccgaa agagaagttg caggaactgc acatctagtc
    47461 agtaaactat cttcacaatt aattgaaaaa agccagttca aatgaactgg cttagttgta
    47521 cttagtgcgc accgccgcca ccgccacctg cgccaaatgg cggtttagca aaccacacca
    47581 gccccagcag tacgaggaat atcccggctg acatccagaa gatctcattg gcggaaataa
    47641 tcagcccctg attggtgatc tgctgggcaa tccagcctga tgcctgctgt tgcgtcatcc
    47701 caagcccttc cagttgactg tacatcgcct gggcattcgg gttgaacggg tttaccgact
    47761 cagtcaactg cgcatggtgc atcgactcgc ggttggtcca catggtcgtg gttatcgacg
    47821 tgccgataga ccccgccagc gttcgcgtaa agttagagag gctcgatgcc gccgccagtc
    47881 gttccggtgg caaaccagac agcgtaatgg tggtcagcgg cataaagaag caggccaccg
    47941 caaacccctg gataaactgc ggccaggccg acgcgccaaa atccatacct ggttcaaagg
    48001 tataggcacg ccagtagaag cagacggcat acataataaa gctgaaggtt accagccgcc
    48061 gcatatccag tttatgcgcg aagcggccga taatcggcga caggatcacc ggaataatcc
    48121 ctaccggcgc agaggccaaa cctgcccagg tcgccgtgta accgtagacc tcctgcaaca
    48181 actgcggcag cagaacaata gcgccgaagt agagcatata cgcgaggctg atacacaagc
    48241 agccgatggt gaagttgcgc gacttaaaca acgacagatc gactatcggg ttatcgtcgg
    48301 tcagctccca gacaatcagg aagcagatag ccaccaccgc caccacggta aggatgataa
    48361 tttcctgtga tgaaaaccag tccagctctt taccgcggtc gagcataatc tgcaggctgc
    48421 cgataccaat aaccagcagt gccagcccca cggcatcaat ccgccgccgt ttggtgcggg
    48481 tttcacgtcc gcgcagagtt tgcagtgtca tcaacaccac cgccacgcca atcggcacgt
    48541 tgatgaagaa tatccagccc cagtggtaat tatcgctgat ataaccgccg aggatcgggc
    48601 cgcaaattgg cgcgacaatc accgtcatcg accacaacgc cagcgcgatc gagcgtttgg
    48661 ctggcgggta gttattcagc aatagacttt gcgaaagcgg gatcaacggc ccggcgacaa
    48721 tcccctgaat cacgcggaag aagatcagca tattcaggct gctggagaca ccacacgccc
    48781 acgacgcaat agcaaaggcg atggtggacc aaaggaacag tttcacttcc ccgacgcgct
    48841 ttgccagcca gccggtaagc gggatcgaga tggcattcgc caccccgaaa gaagtgatta
    48901 cccacgttcc ctggctgagc gatgagccca gattcccggc gatagtgggg atcgccacgt
    48961 tagcaatggt ggagtccagc acctgcatga atgtcgccag tgacagcgca atcgtcataa
    49021 tgaccagttg cgcgccttcc agcggttttt gctgttgcat cacacgcacc tctggattag
    49081 ccagcgttag cttttacgat atcgtcgatc agtttattga caggtgccag gctgatttca
    49141 cgcgcggtac ttaccgcgac cggtgttgaa cgtactttat ttgccagtac ctgaccgtca
    49201 cggttagtgg tattgacgct caccagtgtg gacaaaccaa tgcgcagcgg gtattgctcc
    49261 agctgtttct ggtccagttc gatacgcaca ggtagacgct gaacgacttt gatccagtta
    49321 ccggtcgcat tttgcgccgg aagcagtgag aatgcgctac ctgtgcccat atccagacca
    49381 accactttac cggtgtattt cacatcatcg ccgtaaatat ccgtggtgat agtgaccggc
    49441 tgaccgatac gcatattggc aatctgcgtc tctttaaagt tggcatccac ccacatattg
    49501 gtggctggaa cgaccgccat cagcggcgtc gttgggctaa tttgcgcccc aggctgtacc
    49561 gcacggcggg agacataacc ggtcatcgga ctgacaatac gggtacgctc cagcgccagc
    49621 caggcgttac gtacttcggt ggcagcctgt tgcacggcag gctgatcttc cagtttagtc
    49681 cccagaatca tcgcctgatt ggcattgtat tgttgaatag cgacgtccag ttgcgcctgg
    49741 gcactggtga cggcgtcgcg ggcgtgttgc agctcttcgc gaccaatcag gttggcattg
    49801 cccagcggca cacggcggtt gtagtcgctt tgtgcttttg cgagggcgat tttctgcacc
    49861 tcaatattcg cctgcaactg tttgctgtta atcatcagct ggtgggtttg gcgaacgctg
    49921 gaagccagtg cagttttggc tttttcaaac gcctggcgag catctgtcgg gtcgagagtg
    49981 accagcacgt cgccttcttt tacaaaatcg gtgttatcgg cccagacttt cgtcacgctg
    50041 ccagacacct gagacataat ttgaatctga ttccctgcca cgtatgcgtc atcggtttct
    50101 tcgaaatgac gcagtaccaa aaaccaataa atccctatcg ctacggcaat aattataaag
    50161 agcaaggtga gaaggaggag cagacgctta cgtttgccgc tcttctttac cggttgctgc
    50221 ggggtttgag tctccgcatt tgcgctcata ttgttctcca cgatcttctt aactcatcgg
    50281 ctgagccgac ctgcttgcca aaaaggccag catgttgcga tgctggccgg tcattttttc
    50341 ttttataaat ctggattttt gagcgagatg acgcgttagc tcatcgcttc gagaaccaca
    50401 ccgtcttgtt ccatctggtc gagacgggag agcaatttgc gggtgatttg ctcgagctga
    50461 tctttttctg ttgtgctgag cgcggaccag agttgatgca ggcagttatg ctgcggtggt
    50521 aaaacctcgc gcaaaaactc gtgacctttt tccgttaatt gcagatgcag gcagcggcga
    50581 tcgttatcgc tttcacgacg ttcgatccaa ccgcgttttt ccagttcatc ggcaatacgc
    50641 gtcgcgttgg tacgggatga tccaagagca caacttaatt cagaaggctg aatactgtgg
    50701 ttttcctgag actccagcgt aatcaacgcc ataaacaacg tctcgttaat cccctgagcc
    50761 ttcagcattt tattgcggtt ctccagcagc ttgctttgca tgtgcatgca aagacgagtc
    50821 agaaggatct cctgataagg aaaatcttcg tggcggctgg cgcgaaattt tagcatttgt
    50881 tcaatgggcg taaacgaact atccatttgg gtatgacctc attaatttca gccgatatag
    50941 taacgacagt gacaaataaa gtaaatgtat tgttttagaa aaatgattct tgtgggttat
    51001 ataatcgcca tcactttcca ggcgagccca taggccagcg cactaagcag tgttgggatg
    51061 ataatgctgc gtgttttata gaaactggca cccagtaccg cgaagccgac cagcgtgggc
    51121 acgaaacggc gtgtatcgtg catcacttct ggtgcggtag agacaaccag cagagcgcat
    51181 atcgaggcga tgccaatggt gtcgagcaaa atacctaccg cgccacgttt ggttgggcgg
    51241 gcattaccca cacgcaggcg cagcggcaaa tagcggaagc aataattcgc cgcgccaacc
    51301 agtaacccag gcagcagaac ctcatagctc atcgggcgct ccttgccaga atgcttggat
    51361 taacgcagtg aggcagccac agacaatgcc tgccagaatg gcgacgggaa tagaaaatag
    51421 cgttacgcct gcaagggcac caactaacgc tgcggtaacg caaagagatt gtttgcgctg
    51481 gaaagaggcg agcaggaaac tcataaagag tgccggaagc ataaaaccta atgctgcttc
    51541 aacggcggga taaccttgca gcaagccgct gccagagaat gcccctatta ccgtaccaaa
    51601 tacccacgat gaccatgaac tgaaggcaat gccgatcatc cagttctcgc tccagcggcg
    51661 attattgcgt accagttttg ctgttgcggc ggcaaaaacc tcatccgtca ggccaaacgc
    51721 ccacagggcg gttttcgatt tttgcagacg ctgaataata cggctacgca gtgacgggcc
    51781 atacaacaca tggcgaacat ccattgccat gacggtcagt gcagcaaccc acaaactact
    51841 cccggctgcc agcatcgcgg taatgacgaa ctggctcgcg cctgcataaa tgatgcagga
    51901 gaaaaaaacg ctttcgagag gagagaatcc cagacgggtc gcattcagac cgaacgcaaa
    51961 ggccaccgga atataactaa taacaatcgg taaactgtct ttgcatcctt ccatgaaggt
    52021 cgccgaacca ggagcaggct gtggagtagg gctttccata gagtgtacgc ttaacgattg
    52081 ttaatattat aaatagactg aatgaatatc ttaaccttat cagactgatg ggcttcttaa
    52141 cacccttata agtgtaaagc cacgaaaacg gttgctgatt gcttaatttg cggcttcctg
    52201 acgatgaaaa cctcgccacc agaccagtaa gttaactagg gcaatcgtcg cgccagccag
    52261 acaaacgcca gcccaaccgc catgttgcca ggctgaggct gaaattagcg aaccggcggc
    52321 accgccaata aagtagctgg tcatgtaacc tgcggtcagg cgattacgcg catcagggtg
    52381 tattcgataa attaccgtct ggttagtgat atgcacgccc tgtacggtga gatccagcac
    52441 caggatgccg ataatcagcg ccagtacgga agtgtgacca aaccagatcg ccagccatga
    52501 aagtaatagc agcagcagac cgaaagttgt ggtgtggtgc gatttaccct tatcggcaaa
    52561 accgcccgcc ggacgagcgc ccaacgctcc ggcagctccc gcaagtccaa acagaccaat
    52621 gacaccatcg ctgtagttaa aaggtggagc ggcaagcaaa aaggccattg aggtccagag
    52681 aatgctgaag ttggcaaagg tcaggcagcc cagcaatgcg cgggtgcgga ggatcttatc
    52741 gctgataaac atgctgaaaa cggaacctaa caactgtggg tagttgaggt gggtttctga
    52801 tttcatttgt ggcagaccac gccataatgc tagcgccatc agtgccatta acaccgaggc
    52861 aacccaaaag acggtgcgcc agccgccgag gtttgccagt aacccggcaa cggttcgtgc
    52921 cagcaagatc cccaacagca gaccgctcat aatggtgcca accactttgc cgcgtttatc
    52981 cggtgaagcc agcgtcgctg ccagcggaac cagaatttgt gccacgactg agaataaacc
    53041 ggttaatgcc gtaccgagga tcatcatcgc cagcgactga ctgctggcag tgattaacat
    53101 gccgcctgcc gccagtaagg tcatcgagac aatcaggcgg cggcgttcaa acatatcacc
    53161 gaggggaaca agaaacagca gacctgctgc atagcccaac tgcgcggcgg taacaataaa
    53221 gcctgccgaa ctggcggaaa gggaaaagtt acgcgcgatg gtgtcgagca atggctgggc
    53281 gtaatagtta ctggctaccg cctgaccggt ggcgatagac atcagcacga tcagcgccgg
    53341 gctaagctca tgattaggtt tagtcattat ttttgttcat caggtgatga ttaagctgga
    53401 aatcaataat agcggatgtt gatgaatggg ggcgaaagat agcttgttga aatgtgcgat
    53461 gtggtttgta ggcatgataa gacgcgccag cgtcgcatca ggcattgtgc acagaacggc
    53521 ggatgcggcg aaaacgcctt atccgccata cggaatgagt gtattttgta ggcatgataa
    53581 gacgcgccag cgtcgcatca ggcattgtgc acagaatggc ggatgaggca aaaacgactt
    53641 atccgcccga ataaaaatta cttctgcgct gccagcgcct cattcaccca gccatcgaac
    53701 tgctgctggt gggctttgat ccaaccatca acatggccct gaatatcgcc ttctgaggct
    53761 ttgccgtcat gcataatggc gttctgggcg ttaatatctg ccactggcaa ctgcataatg
    53821 gcaaaaagtt tcgctgctgc cgggtttttc tcggcccagg ctttgttggc aacgatatgc
    53881 atggtgctga ccgggaagcc ataattcgca ccattcggca gtttggtatc ggcgttttta
    53941 tcgcccggca gtgcggagaa cggcacctgc aaccagacca catctttccc tggcttcagc
    54001 tcattactca cccagtacgg cgtccaggtg taataaaaca ccggtttgcc ctctttgtag
    54061 cgactgatgg tgtcggccat catcgctgcg tagttcccct gattatgcgt cacggtgttg
    54121 gtcagttcat acgcggcaag ctggtggttg atcgcacctt cgcagcccca gcctgggtta
    54181 cagccggtta aatccgcttt accgtcgccg ttggtatcga acagtttggc gatcttcgga
    54241 tctttcagtt gtgcgatgtt ggtgattttg tactggtcgg cggttttctt atcgatcagg
    54301 taaccctgtg ccgcgccgtt aacgaatatc ccttcacaat aaaatttctt atcgccaccg
    54361 gcagcttcgt acatgttgtc atgcagcggc gtccagttca cggcggtgaa ggttgcatcg
    54421 ccggaagcaa gtgaggtgta gccaacgttg taatccactt cgctgggttt attgacggta
    54481 taacctaatt tctccagtgc acgactgacc agcagcgtct ggaaggtttc ttcagtgatg
    54541 gtgctctgaa ctggattaac agtaatgcct ttgcccggca gatcggctgc aaaagtttgt
    54601 gtagagataa gcgtggcaaa cgctgtcgca aaaagtacgc tatgtcgcat tgttattcct
    54661 ttttttggca gggtgataat gcccggcgtt accgggcaag tgcagagtta cttaatgaat
    54721 gggcgggtca gcagaccgac agggccagtg gtgtaccagc gacggttgcc gcgactgcgt
    54781 gagtcgcgcc caacggcctg cgtcaggcgg tcgaggataa tggcgaggat cacaatcccg
    54841 acgccgccaa cggtggcaag gcccatatcc agacgaccga taccgcgaag taccatctga
    54901 cccagcccgc cgacggcaat catcgaggcg atgaccacca tagaaagagc cagcatcagc
    54961 gtctggttaa cgcccgccat aatggtcggc atcgccagcg gtaactgaac tttgaacagc
    55021 atctggcgcg ggctggcacc gaatgagcgc gaggcttcaa tcagatccgc cggaacctgg
    55081 ttaatcccca gaatcgtcag acggataatc ggcggcagag caaagatgat cgtcaccact
    55141 acgcccggca cgttaccgat accaaacagc atgacgattg gtaccagata aacaaacgct
    55201 ggcgtggtct gcatggcatc aagcagtgga cgaataattt tcgccgctcg cggacttctc
    55261 gccagccata tccccaacgg caaaccgatg acgatacaga acagcagggc ggttaacacc
    55321 agcgccagag tcaccattgc ctgcgaccag gcaccgattg cgccgatggc aatcagcgaa
    55381 accagcgtcg ccacgcccat tccgaccccg gaaatctgcc aggcgatgag agcgaaaacg
    55441 ataatcgcca ctggcgctgg catacccagc agcaattgct ggaaactgtt gaggatataa
    55501 tcaaccggaa cgcgcacgcc ctggaagacg ggacggaaat gggtaacaac ccagtcgatc
    55561 ccttcagtga cccaactgtc gagcgggatc agcgttttat ggaacggatc gagaatatta
    55621 aaatgctcga cgtttggcgc aggcgtactg gtcagccagt cagcaccacc gccgtcagtc
    55681 ggtgcagtcg tcggtgtacc ccaggcgtct gcggattgcg cggcactgtc cgccgctggc
    55741 gtggtatccc acggattatt ttgatcagcc attatttacc ccctcacgat ctaaagcgcg
    55801 cagcagcatt cctttcgaaa tgatgccgac atactgttgg tcctcgtcga ccacgggcac
    55861 cgcacagggt gcctgtccga catgagagag caactcgcta agaggcgttt gtgcatcgac
    55921 tgctaacggc gcatcaatca gcgccgcatc aagaccttgc tgctgcgtta acgcggtttt
    55981 aagcgaatcg atggagactg cgccgacaaa cttattaccg cgttcgataa cgtagccata
    56041 ttcgcgatct tcatcctgca ataatttcag tgccgaacgt gggccgaagc caggggtttt
    56101 acgaattaag ccattcggtg tccggcgggc aatatctttc gcactgaata cctgactaat
    56161 atcaacgcca cggaagaagg tacggacata atcattcgcc ggattattga gaatttcatc
    56221 cggtgtgccg acctgtacca cttcaccatt ttgcataatg gcaattcggt cgccaatacg
    56281 catggcttca tcgagatcgt gggaaataaa gacaatggtg cgctgatgtt tcgcctgtaa
    56341 ttttaccagc tcatcctgca tctcggtgcg aattaatgga tcgagcgccg agaaggcttc
    56401 gtccattaat aaaatatccg gattaatcgc taacgcgcgg gctaatccca cacgttgacg
    56461 catcccgcca gagagttcat ccgggtagct gtgggcataa ttttccagcc cgacctgacg
    56521 cagtgcatca agggcttttt cccggcgttc ttcggcatta attccggcca attccatacc
    56581 gaacgcagta ttgtccagca cggtcatatg cggcattaag gcaaaggact ggaagaccat
    56641 cgcaatcttt tttctgcgca cctcacggag tttggcatca gatattttgg caatatccac
    56701 accatcaatc agcacttgcc cgcgggtggg ttcaatcagg cgattgagaa ggcgtaccat
    56761 tgtggattta cccgagccgg ataatcccat gatgacaaat atctcgcctt cttcaatggc
    56821 cagactggcg tcttttacgc caagcgatag cccagttttt tccagaattt gttcttttga
    56881 aagtccttgt tcgatatatt tgaatgctcg ctgtggatgc tcaccaaata ttttataaag
    56941 gtttttaatt tctaatttaa ttgccatgca atagaaagat tcctgttatt tgtttatgtc
    57001 gatatattac ccaatggaaa tagttacttt tttctaccct aacatactga gaatctgagg
    57061 caacccctga tggcaaatgt ggcatgaata ttgatgcgag caaatcgccg gaatttcccg
    57121 tgatataagg gctgagagca aatcgagaaa agttgatatt ttttgtcaaa gatagcgaaa
    57181 aaacgcctga atattgtggt ttcaggcgaa taatgcaaca gagtatcagt gtagatcacc
    57241 acaaaatatt tgcgtgataa aaagctattt ggcgggaatt atttccctgt cgcgggtagt
    57301 gatatttttg aaaataacac cctcagaaat tccagtcttc atcttctgtt tcaaccgctt
    57361 tccccatcac ataagaggag cctgaaccgg aaaaaaaatc gtgattttca tcggcattcg
    57421 gcgaaagcgc ggcgaggatt gccggattca cttccgccat ttctgcggga aataaaggtt
    57481 cgtagcccag attcatcaaa gccttattgg cgttgtaaca gagaaacgct ttcacatcgt
    57541 cagcccacgg ggtttcggcg tacagctcat cggtgtattg cagctcgttg tcgtagagtt
    57601 ccagcaacaa atcgaaggcg aaactcttca actcttcacg ttgtcccaga gatatctttt
    57661 ccatgttttt ctgatattta tagcctatgt agtaaccgtg gactgcttca tcacggataa
    57721 tcagacggat caggtctgcg gtattggtca gctttccgcg gctggaaaaa tacatcggca
    57781 gccagaaacc ggaatagaac aaaaaagatt caagaaacac actggcgatt ttctttttca
    57841 gcggatcatc accgcgataa tgttgctgaa taatctgagc ttttcgctgc aacggtgcgt
    57901 tttcttcact ccaggcgtag gcggcatcga catctttggt ctggcacagc gtcgagaaaa
    57961 tcgaactgta agagcgggca tgaaccgctt ccataaagct gatattcgat aataccgctt
    58021 cttcatgagg cgtgagtgca tcgggcatca gcgaaggcgc gccgataaca ttttgcagcg
    58081 tgtcgagcag cgtcaggcca gtaaaaacgc gcatcgtcag ttgttgttct acgacagtta
    58141 atgtctgcca ggcaggaata tcgttcgaca gcggcacctt ttctggtagc cagaaattgc
    58201 tggtcaggcg attccacacc tccagatctt tatcgtcaga tatcttgttc cagttgatgg
    58261 cgctgatacg tgagagtttc atagatattc cttaaagtgc acaggagacg cagccttcaa
    58321 tttcagtgcc ttccagcgcc atctgacgca ggcgaatgta atagagcgtt ttgatacctt
    58381 tgcgccaggc gtaaatctgc gctttgttga tatcgcgagt ggtggcggta tcggggaaaa
    58441 acagcgtcag cgacaaaccc tgatcgacat ggcgagtcgc ttccgcgtag gtgtcgatga
    58501 tcttttctgc gccgatttcg taagcatcct gatacagcgc cagattttcg ttagtcataa
    58561 acggggcagg gtagtaaacg cgtcctgttt tgccctcttt gcgtatctct actttcgcca
    58621 caatcggatg aatactcgac gtagcatggt tgatataaga gatagaaccg gttggcggca
    58681 ccgcctgaag attctggttg taaatgccgt agcgcatcac gtcgtcgcgc agttgcgccc
    58741 acatctcacg ggtaggtaac gtaataccgc tgcgggcaaa cagttcgcca actttcgccg
    58801 ttttcggctg ccagttacct tgcagatatt ggctaaaata ttcaccactg gcataacgtg
    58861 actgtttgaa cccggcgaag gtttcaccgc gttcgcgcgc cagcaacatc gaggtacgca
    58921 gtgcgtgcca ggtgatggca tagaaataga gattggtgaa atccagtgct tccggcgaac
    58981 cataagcgat gccttctcgc gccagatagc cgtgtaaatt catctgcccc agtccgatgg
    59041 cgtgtgaggc ggcatttccg gcttcgatgg acggcacgct gcggatatga ctcatatctg
    59101 acaccgccgt taaaccgcgc acggcagtct ctaccgtgcg ggcaaaatcg ggggaatcca
    59161 tggtgtgcgc aatattcaac gaacctaaat tgcaggagat atcatggcct gtgcgggcat
    59221 agtcgagatt ctcgtcatac tctgaggcac agttgacctg caaaatctct gagcagagat
    59281 tactcatatt tatgcgcccg gcgacagggt tagcacggtt taccgtgtct tcatacatga
    59341 tgtagggata gccggactca aactggattt ctgccagccg ctggaagaaa tcacgggcgt
    59401 tgaggtattt tttgcggatg cgttcatcgg caaccagttc gtcatagtgt tcgctgatgg
    59461 cgatatcggc aaacggcttg ccataaactc gctcaacgtc atagggcgaa aacagcgcca
    59521 tctgcgcatt ctcttttgcc agatggaaag tgatatccgg gatcaccacg cccagcgaca
    59581 gtgttttaat gcggattttt tcgtcggcat tttcccgttt tgtatcgaga aaacgcagaa
    59641 tatcgggatg atgagcatgt aaatagactg cacctgcccc ctgacgcgcg ccgagttggt
    59701 tggcatagga aaatgcgtct tccagcattt tcatcaccgg aattacgcca gaagattgat
    59761 tttcaatacg tttaattggc gcgcccgctt ctcgcagatt cgacagcaaa aatgccacgc
    59821 cgccgccgcg tttcgacagt tgcagtgcgg aatttaccgc ccggccaatc gactccatat
    59881 tgtcttcaat acgcagcaga aaacaggaaa ccagttcgcc gcgctgctgc ttaccgcagt
    59941 tgaggaatgt cggcgtggct ggctgaaagc gtcctgacag catttcatcg gtcagttgca
    60001 acgccagcgt cgcatcgcct tgtgccagcg tcagcgccac catcgtgact cgatcggcaa
    60061 aatcttccag ataacgttta ccgtcgaatg tcttcaacgt atagctggtg taaaacttcc
    60121 atgccccgag gaatgtctgg aaacgaaaac cgctggtgtg tgcgtgggca aacagcgtaa
    60181 tgacaaagtc gcgagagtag cgattaagaa cgctttcatc atagtaacct tcgttgacca
    60241 gccagttcag gcgctgctgc tggctactga aggtcacact gttcggacgc acatgcgtcg
    60301 ccataaaggc gtcaacggcc tggcggtctt tatcgaactg aatgcgacct gcgctatcgt
    60361 agaggttaag catcgcattc agcgcgtggt aatccatcgt ttcctgcgtc aggcattctg
    60421 cggttgtcgt tgccaaaatt cggttactcc tttacgaacg ttttcgatat cgctttgcgt
    60481 acccatgagt tcaaaacggt acagccacgg cacgccgcat ttccgggcaa tcacatctcc
    60541 ggcgcggcca tacgcctcac caaagttgcg attaccagaa gcaataacgc cgcgaagcaa
    60601 cgcccggttg tgctcgtcgt ttaaaaagcg aattacctat cgtggcaccg cgccagccgt
    60661 accgccgccg ccgtaagagg gcacgatcag gatgtaaggc tcgtctacct gaatccgttc
    60721 ccgctcattg agcgggatgc gcaccgcggg cagacctaaa cgttcgataa aacgctgcgt
    60781 gttttcggag ctgctggaga agtagacgag ctggctcatg cactggccgc gtgtggcgct
    60841 ggatgcagac ggttaatcat gtccggacgg aaaccagacc agctaagatc gccagcaatc
    60901 actaccggca actgacgaaa gccctgagca cgcaacgctt ccgctgcttc aggaacgcga
    60961 tcgacattaa tcatttcaaa atcaaagccc cggttttcca tcgcccgttt ggtggcgtgg
    61021 cactgaacgc aatcgttacg agtgtaaata gttatgcgca tgattcgtat ttccgtttaa
    61081 aatgaagata cggcgcgatg atacgcgtcg ggttgtctct ctgttgatac agagatacta
    61141 gatgtagttg aaaaaagatt caaccacaca atatatagca aatagtgatc gaaattaccc
    61201 tagatatgag cgtgacgggg gagggggatt tttgtgattc accaggcaaa aagaaacccc
    61261 gaagacaggc ttcggggtta aagacgcgta tttattatca tttttgcact acgatttgcg
    61321 catgcttaac agtgcgccga taaaaatacc taccgcagct gctgtaccca cgctacacca
    61381 gggtctttca cgaacaaaag aatccgcgca gccaacggca tcgcgtgcgg cttgctggac
    61441 gcgagtacga ccatgcattc gcgccctggt ttctttcagc aatgcctgag ctttactgcg
    61501 tgcggcttca gcttcccctt tggcgtcgct gccccaggac ttcaatacag attcgaggct
    61561 gtcagctaat tgattgacat cattctggat atcctgcacg ccatcatcaa cgtcgttgcg
    61621 gttcggtcgg ttaaacatat gatcccccat tagaattcga tgtaattttc agtgtagacc
    61681 ataattttct taactgaggt tcatcgcggc tttatggact tttctgaaag cgttgtgaat
    61741 gcgcaatcag gtaaggtagg cccgcggttg ataataacga ggatcaaata tgtatttacg
    61801 accagacgag gtggcgcgcg tacttgaaaa agtcggtttt actgtcgatg tggtaaccca
    61861 gaaagcttat ggttaccgcc gtggggaaaa ttatgtctat gttaatcgcg aagcgcgaat
    61921 gggacgtacc gcactggtaa ttcatccaac attaaaagaa cgtagttcga cactggctga
    61981 acccgcttcc gatattaaaa cttgcgatca ttatcaacaa tttccgctct atttagcagg
    62041 cgagcgacac gagcattacg gtatcccgca tggcttcagt tcgcgtgttg cgcttgaacg
    62101 ttatttgaat ggtttatttg gcgaagccag ttaaagacgc gactggcgat gccagtcgcg
    62161 aaaagaagag tcaggctttt acctgctggt aatggctgac tttaaacagt cggcggcaat
    62221 aatcgaggaa gtagccataa accgccccca tcaacatcga aacaacgatg tttgaactga
    62281 ccgccgccat aatctgatgc cagtctgcgc ccaccactaa caagatcgcc acatacaccg
    62341 gtgactggaa cgtcacataa gccaggatat cagccagatt ttttatccag cccgacgggc
    62401 taactttgcg tgccgcgcgc ataaatagat cgcggtacat accgtatggc catgcaatta
    62461 agatgttcac cggaatcgct accaatctgg aataaaaaga ctgttcgaag ctcattccgg
    62521 agaggaaaac ttcaatacac atgttcacga cagaacagta aacaaccatc gcgaacgtat
    62581 ctgcaactgc atgacgcaag cgtgactgcg gtgagaacat gctaatgctc cttaataaaa
    62641 gcgtaatggg atgttaatgg agatgaaagc agtggatctc attgccatct gcattttcgc
    62701 atagcgtata tcgctggcta tgactattca attagtgaaa aatttttatt tagtcagttt
    62761 ttgtagataa aatattccgt aacggctcgt tttttgtaca ttgtggattt ttacatgctg
    62821 atatgattta ttttcaatta aatcaattag atatgacctt gcctttaatt tcactaatga
    62881 aagtttgggg gtgaggtaac gctataagcg tagagaattc ggtgtatgtc gtgaccgtgt
    62941 catttacagc atttttgatg ccagaaaatg tacattaaca gcagaaagca ccagtgaggg
    63001 ggg
//
LOCUS       DADMKF010000005        60958 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.005, whole genome shotgun sequence.
ACCESSION   DADMKF010000005 DADMKF010000000
VERSION     DADMKF010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 60958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60958
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.005"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            <1..468
                     /locus_tag="JNC24_000287"
     CDS             <1..468
                     /locus_tag="JNC24_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY9157139.1"
                     /translation="SGTSVTGSTVKMGEYSAEQIRNGATPVPFDISLQNCVRVTNIET
                     KLVSTKVGTENGQLLGNTLTGNDAAKGVGVLIEGLATSKNPLMTLKPNDSNSVYKDYD
                     PRGKDDTTGGVYPDQDTGITYPLHFQATLQQDGTIPIEAGEFKATSTFQVTYP"
     gene            581..1375
                     /gene="panB"
                     /locus_tag="JNC24_000288"
     CDS             581..1375
                     /gene="panB"
                     /locus_tag="JNC24_000288"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HAY9157140.1"
                     /translation="MKPTTIASLQKCKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTASVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGDEAG
                     DQLLSDALALEAAGAQLLVLECVPIELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            1387..2238
                     /gene="panC"
                     /locus_tag="JNC24_000289"
     CDS             1387..2238
                     /gene="panC"
                     /locus_tag="JNC24_000289"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HAY9157141.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHDGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIIAIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKIVELA"
     gene            complement(2530..3426)
                     /locus_tag="JNC24_000290"
     CDS             complement(2530..3426)
                     /locus_tag="JNC24_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HAY9157142.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEESLKGHSTDVLYSVQMQGNPGYLHVVIEHQSKPDKKMVFRMMRYSIAAM
                     HRHLEADHDKLPLVVPILFYQGEATPYPLSMCWFDIFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGESMMTLAQWFEEKGIEKGIQQGRQEERQEFAQRFLSKG
                     MSREDVAEMTNLSLAEIDRLIN"
     gene            3700..4080
                     /gene="panD"
                     /locus_tag="JNC24_000291"
     CDS             3700..4080
                     /gene="panD"
                     /locus_tag="JNC24_000291"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HAY9157143.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(4084..5313)
                     /locus_tag="JNC24_000292"
     CDS             complement(4084..5313)
                     /locus_tag="JNC24_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAY9157144.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWAQIGDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLVTWKDTPVYNAPSVGSAPFGVLADNLRYPILHKLKDRLNQTWYQVRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNKINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATAFVVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLETMSRLVSNQPQG"
     gene            complement(5377..5817)
                     /locus_tag="JNC24_000293"
     CDS             complement(5377..5817)
                     /locus_tag="JNC24_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAY9157145.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRERIVELGGPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(5922..6692)
                     /locus_tag="JNC24_000294"
     CDS             complement(5922..6692)
                     /locus_tag="JNC24_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY9157146.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(6689..7615)
                     /locus_tag="JNC24_000295"
     CDS             complement(6689..7615)
                     /locus_tag="JNC24_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAY9157147.1"
                     /translation="MTIALELQQLKKTYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            7724..8386
                     /gene="can"
                     /locus_tag="JNC24_000296"
     CDS             7724..8386
                     /gene="can"
                     /locus_tag="JNC24_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HAY9157148.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(8427..8975)
                     /gene="hpt"
                     /locus_tag="JNC24_000297"
     CDS             complement(8427..8975)
                     /gene="hpt"
                     /locus_tag="JNC24_000297"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HAY9157149.1"
                     /translation="MVRDMKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVG
                     LLRGSFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIV
                     EDIIDSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGY
                     GIDYAQRYRHLPYIGKVILRDE"
     gene            9169..11559
                     /locus_tag="JNC24_000298"
     CDS             9169..11559
                     /locus_tag="JNC24_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone-dependent
                     dehydrogenase"
                     /protein_id="HAY9157150.1"
                     /translation="MAINNTGSRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWCSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGSILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQINADNVHNLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPVIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPKKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLIPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKINEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(11606..13156)
                     /gene="cueO"
                     /locus_tag="JNC24_000299"
     CDS             complement(11606..13156)
                     /gene="cueO"
                     /locus_tag="JNC24_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HAY9157151.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGEKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLAEETTLHW
                     HGLEVPGDVDGGPQGIIPPGGKRTVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFNADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVSELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGM
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHNAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            13322..13669
                     /locus_tag="JNC24_000300"
     CDS             13322..13669
                     /locus_tag="JNC24_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HAY9157152.1"
                     /translation="MKTFFRTVLFGSLMAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            13775..14641
                     /gene="speE"
                     /locus_tag="JNC24_000301"
     CDS             13775..14641
                     /gene="speE"
                     /locus_tag="JNC24_000301"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HAY9157153.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGIVSFCRQYLPNHNAGSYDDPRFKLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSYRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            14657..15451
                     /gene="speD"
                     /locus_tag="JNC24_000302"
     CDS             14657..15451
                     /gene="speD"
                     /locus_tag="JNC24_000302"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HAY9157154.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(15489..15851)
                     /locus_tag="JNC24_000303"
     CDS             complement(15489..15851)
                     /locus_tag="JNC24_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacL family protein"
                     /protein_id="HAY9157155.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDGEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(16026..18623)
                     /gene="acnB"
                     /locus_tag="JNC24_000304"
     CDS             complement(16026..18623)
                     /gene="acnB"
                     /locus_tag="JNC24_000304"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HAY9157156.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVDVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLNSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLADIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            18978..20735
                     /locus_tag="JNC24_000305"
     CDS             18978..20735
                     /locus_tag="JNC24_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HAY9157157.1"
                     /translation="MKMTLPFKPHVLALICSAGLCAASAGLYIKSRTVEAPVETQSTQ
                     QTAPDITAVTLPATVSAPPVTPAVVKSAFSTAQIDQWVAPVALYPDSLLSQVLMASTY
                     PANVAQAVQWSHDNPLKQGDAAIQAVSDQPWDASVKSLVAFPQLMALMGENPQWVQNL
                     GDAFLAQPQDVMDSVQRLRQLAQQTGSLKSSTEQKVITTTKKAVPVKQTVTAPVIPSN
                     TVLTANPVITEPATTVISIEPANPDVVYIPNYNPTVVYGNWANTAYPPVYLPPPAGEP
                     FVDSFVRGFGYSMGVATTYALFSSIDWDDDDHDHHHHDDDDYHHHDGGHRDGNGWQHN
                     GDNINIDVNNFNRITGEHLTDKNMAWRHNPNYRNGVPYHDQDMAKRFHQTDVNGGMSA
                     TQLPAPTRDSQRQAAASQFQQRTHAAPVITRDTQRQAAAQRFNEAEHYGSYDDFRDFS
                     RRQPLTQQQKDAARQRYQSASPEQRQAVREKMQTNPQNQQRREAARERIQSATPEQRQ
                     VFKEKVQQRPLNQQQRDNARQRIQSASPEQRQVFREKVQESRPQRLNDSNHTVRLNNE
                     QRSAVRERLSERGARRQER"
     gene            complement(20806..22230)
                     /gene="lpdA"
                     /locus_tag="JNC24_000306"
     CDS             complement(20806..22230)
                     /gene="lpdA"
                     /locus_tag="JNC24_000306"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HAY9157158.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(22555..24447)
                     /gene="aceF"
                     /locus_tag="JNC24_000307"
     CDS             complement(22555..24447)
                     /gene="aceF"
                     /locus_tag="JNC24_000307"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HAY9157159.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEAAPAAAPASAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIR
                     RLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKV
                     DFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAK
                     RKLDVKLTPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVV
                     PVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVN
                     APEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSD
                     IRRLVM"
     gene            complement(24462..27125)
                     /gene="aceE"
                     /locus_tag="JNC24_000308"
     CDS             complement(24462..27125)
                     /gene="aceE"
                     /locus_tag="JNC24_000308"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HAY9157160.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKEISTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYIPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(27286..28050)
                     /gene="pdhR"
                     /locus_tag="JNC24_000309"
     CDS             complement(27286..28050)
                     /gene="pdhR"
                     /locus_tag="JNC24_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HAY9157161.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            28591..29964
                     /gene="aroP"
                     /locus_tag="JNC24_000310"
     CDS             28591..29964
                     /gene="aroP"
                     /locus_tag="JNC24_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HAY9157162.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAIVAMIIFGGWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(30007..30861)
                     /gene="ampE"
                     /locus_tag="JNC24_000311"
     CDS             complement(30007..30861)
                     /gene="ampE"
                     /locus_tag="JNC24_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HAY9157163.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(30858..31409)
                     /gene="ampD"
                     /locus_tag="JNC24_000312"
     CDS             complement(30858..31409)
                     /gene="ampD"
                     /locus_tag="JNC24_000312"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HAY9157164.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRPDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDRYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            31497..32390
                     /gene="nadC"
                     /locus_tag="JNC24_000313"
     CDS             31497..32390
                     /gene="nadC"
                     /locus_tag="JNC24_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HAY9157165.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVSTRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            32593..33033
                     /gene="ppdD"
                     /locus_tag="JNC24_000314"
     CDS             32593..33033
                     /gene="ppdD"
                     /locus_tag="JNC24_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HAY9157166.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWARNCNIQSDSALQQACEDVFRFDDAN"
     gene            33043..34429
                     /gene="gspE"
                     /locus_tag="JNC24_000315"
                     /pseudo
     CDS             33043..34429
                     /gene="gspE"
                     /locus_tag="JNC24_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
     gene            34419..35621
                     /gene="hofC"
                     /locus_tag="JNC24_000316"
                     /pseudo
     CDS             34419..35621
                     /gene="hofC"
                     /locus_tag="JNC24_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414648.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
     gene            complement(35656..36699)
                     /gene="guaC"
                     /locus_tag="JNC24_000317"
     CDS             complement(35656..36699)
                     /gene="guaC"
                     /locus_tag="JNC24_000317"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HAY9157167.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHYSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVQEQENRIFNNL"
     gene            36924..37544
                     /gene="coaE"
                     /locus_tag="JNC24_000318"
     CDS             36924..37544
                     /gene="coaE"
                     /locus_tag="JNC24_000318"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HAY9157168.1"
                     /translation="MRYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGANMIAADGTLQRRALRERIFANPEEKNWLNALLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            37544..38287
                     /gene="zapD"
                     /locus_tag="JNC24_000319"
     CDS             37544..38287
                     /gene="zapD"
                     /locus_tag="JNC24_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HAY9157169.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELDRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            38297..38494
                     /gene="yacG"
                     /locus_tag="JNC24_000320"
     CDS             38297..38494
                     /gene="yacG"
                     /locus_tag="JNC24_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HAY9157170.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(38594..38983)
                     /gene="mutT"
                     /locus_tag="JNC24_000321"
     CDS             complement(38594..38983)
                     /gene="mutT"
                     /locus_tag="JNC24_000321"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HAY9157171.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGEPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(39043..41748)
                     /gene="secA"
                     /locus_tag="JNC24_000322"
     CDS             complement(39043..41748)
                     /gene="secA"
                     /locus_tag="JNC24_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HAY9157172.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(41810..42322)
                     /gene="secM"
                     /locus_tag="JNC24_000323"
     CDS             complement(41810..42322)
                     /gene="secM"
                     /locus_tag="JNC24_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secA regulator SecM"
                     /protein_id="HAY9157173.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(42553..43470)
                     /gene="lpxC"
                     /locus_tag="JNC24_000324"
     CDS             complement(42553..43470)
                     /gene="lpxC"
                     /locus_tag="JNC24_000324"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HAY9157174.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(43571..44722)
                     /gene="ftsZ"
                     /locus_tag="JNC24_000325"
     CDS             complement(43571..44722)
                     /gene="ftsZ"
                     /locus_tag="JNC24_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HAY9157175.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(44783..46045)
                     /gene="ftsA"
                     /locus_tag="JNC24_000326"
     CDS             complement(44783..46045)
                     /gene="ftsA"
                     /locus_tag="JNC24_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017374018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HAY9157176.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(46042..46872)
                     /gene="ftsQ"
                     /locus_tag="JNC24_000327"
     CDS             complement(46042..46872)
                     /gene="ftsQ"
                     /locus_tag="JNC24_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HAY9157177.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLGWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(46874..47794)
                     /locus_tag="JNC24_000328"
     CDS             complement(46874..47794)
                     /locus_tag="JNC24_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase B"
                     /protein_id="HAY9157178.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSMDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(47787..49262)
                     /gene="murC"
                     /locus_tag="JNC24_000329"
     CDS             complement(47787..49262)
                     /gene="murC"
                     /locus_tag="JNC24_000329"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HAY9157179.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLMNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLIGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(49316..50383)
                     /gene="murG"
                     /locus_tag="JNC24_000330"
     CDS             complement(49316..50383)
                     /gene="murG"
                     /locus_tag="JNC24_000330"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HAY9157180.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHHLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(50380..51624)
                     /gene="ftsW"
                     /locus_tag="JNC24_000331"
     CDS             complement(50380..51624)
                     /gene="ftsW"
                     /locus_tag="JNC24_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HAY9157181.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSSVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(51624..52940)
                     /gene="murD"
                     /locus_tag="JNC24_000332"
     CDS             complement(51624..52940)
                     /gene="murD"
                     /locus_tag="JNC24_000332"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HAY9157182.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIAQAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDAECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     QVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQLGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(52943..54025)
                     /gene="mraY"
                     /locus_tag="JNC24_000333"
     CDS             complement(52943..54025)
                     /gene="mraY"
                     /locus_tag="JNC24_000333"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HAY9157183.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(54019..55377)
                     /gene="murF"
                     /locus_tag="JNC24_000334"
     CDS             complement(54019..55377)
                     /gene="murF"
                     /locus_tag="JNC24_000334"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HAY9157184.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGTEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSVGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(55374..56867)
                     /gene="murE"
                     /locus_tag="JNC24_000335"
     CDS             complement(55374..56867)
                     /gene="murE"
                     /locus_tag="JNC24_000335"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HAY9157185.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKAKDEATDGEIREMHGVPVIYLSQLNERLSALAGR
                     FYHEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENT
                     TGSAVDVQHELAGLVEQGATFCAMEVSSHGLVQHRVAALKFAASVFTNLSRDHLDYHG
                     DMEHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWL
                     KAIDVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKT
                     AARLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDR
                     DKGKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAV
                     TCAVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGVIA"
     gene            complement(56854..58620)
                     /locus_tag="JNC24_000336"
     CDS             complement(56854..58620)
                     /locus_tag="JNC24_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HAY9157186.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPGSIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(58636..59001)
                     /gene="ftsL"
                     /locus_tag="JNC24_000337"
     CDS             complement(58636..59001)
                     /gene="ftsL"
                     /locus_tag="JNC24_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HAY9157187.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(58998..59939)
                     /gene="rsmH"
                     /locus_tag="JNC24_000338"
     CDS             complement(58998..59939)
                     /gene="rsmH"
                     /locus_tag="JNC24_000338"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HAY9157188.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAVATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(59941..60399)
                     /gene="mraZ"
                     /locus_tag="JNC24_000339"
     CDS             complement(59941..60399)
                     /gene="mraZ"
                     /locus_tag="JNC24_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HAY9157189.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAVGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
BASE COUNT        14596 a        16854 c        14998 g        14510 t
ORIGIN      
        1 agtggaactt ctgtgactgg ttctacggta aaaatgggtg aatatagtgc agaacaaatc
       61 agaaacggtg ccacaccggt tccttttgat atttcacttc aaaactgcgt tcgtgtgact
      121 aatattgaga caaaattagt ttcaacaaag gttggtactg aaaacgggca actccttggt
      181 aatactctca caggtaatga cgcagcaaaa ggagtcggtg tactcataga aggtttagca
      241 actagtaaaa atcctctaat gacattgaaa cctaatgatt caaattctgt ttataaagat
      301 tacgacccga gaggcaaaga cgatacaaca ggaggagttt acccggatca agatacaggt
      361 ataacatacc ctctccattt ccaggccacg ctacaacagg atggaactat accaatagaa
      421 gctggtgagt ttaaagccac cagtactttc caggtaacct acccttaata agtccaccgc
      481 accgccatca tctggcggtg cggtaattga taaatcattg cccccaccaa tgtgactgat
      541 aaaatgccag gcaacacgac atcatttatc aggacacgtt atgaaaccga ccaccattgc
      601 ttcacttcag aaatgtaagc aggataaaaa gcgtttcgcg accatcaccg cttacgacta
      661 cagcttcgcg aaactatttg ctgatgaagg acttaacgtc atgctggtag gcgactcgct
      721 gggcatgacg gttcagggtc acgactccac cctgccggtg accgttgccg atatcgccta
      781 ccacactgcc tccgtacgtc gtggtgcgcc aaactgcctg ctgttggctg acctgccgtt
      841 tatggcgtat gccacgccgg aacaagcctt tgaaaacgcc gcaacggtta tgcgtgccgg
      901 tgccaatatg gtcaaaattg agggcggtga gtggctggtc gaaaccgtaa aaatgctgac
      961 cgaacgtgcc gttcctgtat gtggtcactt aggtttaacc ccacagtcag tgaatatttt
     1021 cggtggctac aaagttcagg ggcgcggcga tgaagcgggc gatcaactgc tcagcgatgc
     1081 attagcctta gaagccgctg gggcacagct gctggtgctg gaatgcgtgc cgattgaact
     1141 ggcaaaacgt attaccgaag cactggcgat cccggttatt ggcattggcg caggcaacgt
     1201 cactgacggg cagatcctcg tgatgcacga cgccttcggc attaccggcg gtcacattcc
     1261 taaattcgct aaaaatttcc tcgccgaaac gggcgacatc cgcgcggctg tgcggcagta
     1321 tatggctgaa gtggagtccg gcgtttatcc gggcgaagaa cacagtttcc attaaggagt
     1381 cacgttgtgt taattatcga aaccctgccg ctgctgcgtc agcaaattcg ccgcctgcgt
     1441 atggaaggca agcgcgtggc gctggtgcct actatgggta atctgcacga tggccatatg
     1501 aagctggtcg acgaagccaa agcccgcgcc gatgtggtcg tcgtcagtat tttcgttaac
     1561 ccgatgcagt tcgaccgccc ggaagatctg gcacgttatc cacggacctt gcaggaggac
     1621 tgcgagaagc taaacaaacg taaagtggat ttagttttcg ccccttcggt aaaagagatc
     1681 tacccgaacg gtactgaaac ccatacttac gttgacgttc ccgggctttc gaccatgctg
     1741 gaaggcgcga gccgtccggg gcattttcgc ggcgtttcga ctatcgtcag caagctgttt
     1801 aacctggtcc agccggacat cgcctgcttc ggtgaaaagg actttcagca actggcgcta
     1861 atccgcaaaa tggttgccga tatgggcttc gatattgaaa ttgtcggcgt cccgattatg
     1921 cgcgccaaag atggccttgc gctcagttcc cgtaatggtt atctgacggc ggaacaacgc
     1981 aaaattgcgc ccggtctgta caaagtttta agttcaattg ccgacaaatt gcaggctggc
     2041 gagcgggatc ttgatgaaat tattgccatt gcagggcaag aactgaatga aaaaggcttc
     2101 cgcgccgatg atattcagat tcgcgatgcc gacacgttgc tggaagtctc tgaaaccagc
     2161 aaacgagctg taattctggt agccgcctgg cttggcgatg cccgcctgat cgacaacaaa
     2221 atcgtcgaac tggcgtaatg cttttactct aaataattca agttgcagga aggcggcaag
     2281 cgagtgacaa attcgtcagg aacgaatttg cccagccgca ggctggcctt cggtgagaga
     2341 catggatgtc tctcattaat ccccaggagc ttacataagt aagtgactgg ggttagcgaa
     2401 cgcagacgca gcacatgcaa tttaaagtat gacgagtata actggcgcta cggctgatgg
     2461 cgccaattat taatttgccc cacgtcatgg cgatggcgtc tgtaagcaat tattcataac
     2521 gcagacaact tagttaatca gcctatcaat ctcagcaaga gataaatttg tcatctctgc
     2581 aacgtcttcc cgagacatcc ctttactcag aaaacgctgg gcgaattctt gcctttcctc
     2641 ctgtcttccc tgctgaatcc ccttctcaat ccctttctct tcaaaccact gcgccagcgt
     2701 catcatagac tcccctcccg tttccctgtc tctcagcaca ccataaaaca aatccgcttg
     2761 ttcagtatga ccgcgttgca gcatatagtt ttgcatggca actaactgac ttccgctagt
     2821 gtacccttcg tcaatcagcg tgaccagttg ctccagcaat aacattaagt cgcgctggcg
     2881 aatatgtttt tgcagtagtt cgagaatcgc aatccgccga tgttgcatga tttcgtcatc
     2941 cggtgtgatg gtgatatcca ccagcgggaa aggactgtta tagacgcgtc gtgccagctc
     3001 cggcgagtaa aatatatcaa accagcacat tgagagcgga taaggtgtgg cctcgccctg
     3061 ataaaacagt atcggcacca ccagcggcag cttatcgtga tccgcctcca gatgccggtg
     3121 catggcagca atagaataac gcatcatgcg aaagaccatt ttcttatccg gcttgctttg
     3181 gtgttcaatc acaacatgca gataaccggg attgccctgc atttgcacgg aatagagcac
     3241 gtccgtgctg tgtcctttca ggctctcttc aatgaaactc cccgactcta aatgaagcgt
     3301 gttgaggtca caaagttctc gtaattccac tggcaaatgt atctccagaa agtcgcgagc
     3361 cgtctccgcg tgcattaaaa attgtttaaa cactgcatca tgcggtgtgg tactcggtgc
     3421 atccatcttc cctgccttcc gtgttgtctt gcgtgaaggc agtatccagt caaggcgtgc
     3481 catcgtcagc cacctcttta gtgtttaccg agcagcgttc agagaaattt caattggtta
     3541 cagccgttac atggcacgct tcggcacggt acgcaaactg ctgatgtaaa tgccgtcaga
     3601 tgagtagaca ctaaacaaaa atcgggcaat actgcgtgag aatttcctca aagcaggcat
     3661 cgcctgcttc gttaacgaca gggtagaaag gtagaagtta tgattcgcac gatgctgcag
     3721 ggcaaactcc accgcgtgaa agtgactcat gcggacctgc actatgaagg ttcttgcgcc
     3781 attgaccagg attttcttga cgcagccggt attctcgaaa acgaagccat tgatatctgg
     3841 aatgtcacca acggcaagcg tttctccact tatgccatcg cggcagaacg cggttcgaga
     3901 attatttctg ttaacggtgc ggcggcccac tgcgccagtg tcggcgatat tgtcatcatc
     3961 gccagcttcg ttaccatgcc agatgaagaa gctcgcacct ggcgacccaa cgtcgcctat
     4021 tttgaaggcg acaatgaaat gaaacgtacc gcgaaagcga ttccggtaca ggttgcttga
     4081 ttgttatccc tgcggctggt tactcaccag ccgcgacatc gtctccagcg aatccgttct
     4141 taagatataa agtcgtttta gtaacaacgg attatccccc ggttttactt tgcctttcat
     4201 ggttgtcacc gccaggtgaa atccggcatc gtttgctgcc ttcacggctt tgtcattaaa
     4261 tccgccaaac ggatacgaaa gataccagac atgcggatta aattgcgcca gagcgcggcg
     4321 tgaacgtgca aaatcaaaca gaatattgtg ctcactacgg ctcagtaata tgggtcggcg
     4381 atagccatcc acccgatgca aaaaatgggt atgtgactga aaatcaaaaa catcgcgaat
     4441 ttcgttaagc tcagaaacgc tcataaattg cagcgatttt gggttccact tctgcgggtg
     4501 acgtttgatg cgcgaggtaa caacaaacgc cgtcgccttc atgccatatt gtttcaatac
     4561 aggatacgca tagcggctca ccgacttgag gccatcatca aaggtaatca ccaccgctcg
     4621 cgcagggaga ttgatcttat tcttcacgta accttccagc tgcgccatgc tcagtgtcgc
     4681 gtatcccctg tcgcgcagcc aggccatttg gttattgaaa gcgcgtaccg aggtggtcgt
     4741 cgaagtatgg cgaaaacggg tgttttcttc gtcgcgcaga atatggtgat aagttagcac
     4801 cggaaggcca ttatcgggtt gggcatccag cgcgctgata taggccagtc gatcgccaat
     4861 acggacctga taccaggttt gatttaacct gtctttcagt ttatgcaaaa tcgggtagcg
     4921 caaattgtcc gccaataccc caaatggcgc actgcccaca ctcggcgcgt tatagaccgg
     4981 cgtatctttc caggtaacta agttctgatt actcagcggc ttgttgaggt cacccaaacc
     5041 gtcttcaact ttttgtcgcc cctgaaccgg ctcgagatgc cctttatcga taaacccttt
     5101 accaaagcca aaattaaatg cgtaataact tgcggcagtg ggctccacgg caatgatttg
     5161 tccggcccga atattcccca cggtcaccat cttgtcgcca atttgcgccc agaccgcagc
     5221 attttcgatg gtttgcatat aacgggcagg taacgcggca ctgacacttg cggtaaacag
     5281 catcagcagg agaataacag cttgtttgta catgaaaaaa taaccgaggg tgaaacaacg
     5341 cttttattgt agcaaaagcg ctataagaat cgtgggttaa tactcataca aattatgttt
     5401 gaggacgaaa ggcggatttt tttgttgttg gtgccaaaga ctactcacct ccggcccacc
     5461 cagttcgaca atacgctcgc gaaactctga actggtcatg gtttcacggc aagccatcat
     5521 ctgttcaatt aacggtaacg tgacaccaga aatcacttcg caacgggagt gtttgtggct
     5581 taataatgaa gccacgcggt acggtggcgc tccggctata tcggttaaga agatgacacc
     5641 ctcaccagag tccgcttcat gcagagcatc gcacatcatg cggctgagca tattagagct
     5701 taacccacgc cagaaattca cggcccggca ctgaagaagc gccccatgtt ttttctccag
     5761 cgcatccagt atctcttgcg cccggtcatc gtgacaggta attacccaac ctaacatcgc
     5821 cttcctcctt agccgaaaat cagtctaccc tgaccattgt tgataaaggg tgatagccgt
     5881 caaaactcct ctcccccaaa tccagaggag aggaaatagc cttagctacg caaaccacgt
     5941 ccacgttgga tcagcgacca acagatcaaa taaaacgcca caataaagac caccagtacg
     6001 ccaaaggtag tgaccagcgg aacatcattg ataccgagga agccgtagcg gaaaccactg
     6061 atcatataaa cgattgggtt caggtgcgac agcccttgcc agaacggcgg caacaaagtc
     6121 agtgagtaaa agaccccacc caaatacgtg agtggcgtta acacaaaggt tggcaccagg
     6181 ctgatgtcat cgaacgtttt ggcaaacaca ccgttcagca aacccgcaag ggagaacaac
     6241 accgccgtga gcaccagggt taaggcaacg aatacccacg aatgcacctg aaacggcaca
     6301 aaaaacagtg aaattgccgt caccagaatg ccaacaaaca gaccacgcgc cacgccaccg
     6361 ccgacatatc cggcaataat gacgtgagtc ggaaccggcg ctaccagcag ctcttcaata
     6421 ttacgctgga acttggcacc aaaaaatgat gacgcaacgt tggcgtaggc attggtgatc
     6481 accgacatca tgatcagccc cggtacgatg aactgcatat agctgaagcc atgcatatcg
     6541 ccaatacgcg aaccaatcag gttaccgaag ataataaagt aaagggtcat ggtgatgact
     6601 ggcggcacca gcgtctgcac ccagatacgc ataaagcgat ggatctcttt cgcccagatg
     6661 ctttttagcg ccacccagta aagatgcatc atgcgcgatc tccttgtttt tcattaacca
     6721 gtgaaacaaa cagctcttcc agacggttag ctttgttacg catacttaat acctgaatgc
     6781 cctgctcact taactgcgta aatacgctgt tgatcccctg ctcacgcagc acttcaactt
     6841 ccagcgtcgc ggtatcgacc agtcgatact gatagccatc gagcttcggt aacggacttt
     6901 tcggtgcgag atcgagaata aaggtttccg atttcagctt cgccagcagc gccttcatcg
     6961 aggtattttc caccagctca ccgtgttgaa taatgccgat attgcggcac agcatttctg
     7021 cttcttccag gtagtgtgtg gtgagaatga tggtggtgcc tttgtcgttt aaatccttca
     7081 aaaagcccca cattgagcgg cgaagttcaa tatccacgcc tgcggtcggt tcgtcgagaa
     7141 tcagtagttt aggttcatgc attaacgcac gggcaatcat taaacggcgc ttcatcccgc
     7201 cagataacat acgcgcacgt tcgttgcgtt ttccccatag atcgagttgt ttaagatatt
     7261 tttcgctgcg gatgtacgcc tctttgcgct ccacaccgta gtaccctgcc tgattcacca
     7321 caatttgctg cacggtttca aacgggttga agttaaattc ctgcggcacc agtcccaact
     7381 gacgtttagc gttcacgaca tctttctcga gatcgtaacc aaatacgctg acccgcccgg
     7441 aggttttatt taccagagag ctgataatac cgatagtggt cgatttcccg gccccgttcg
     7501 gcccgagaag cgcataaaaa tcacccgctt cgacctgcaa atctattcca cgaagcgcct
     7561 gaacgccgcc tggataggtt tttttaagct gttgaagttc cagtgcaatg gtcataaatt
     7621 tttacttacc ttacgttctt acactttatc tgtggtttaa atcgtcccgg agttgcccta
     7681 tattagccaa acgtaattat ttggttacag gtcgttaacc tccatgaaag acatagatac
     7741 actcatcagc aacaatgcac tatggtcaaa aatgctggtg gaagaggatc ccgggttttt
     7801 tgagaaactg gcacaagcgc aaaaaccgcg ctttctatgg attggatgtt ccgacagtcg
     7861 cgttcctgca gaacgtttaa ccggtcttga gccgggcgaa ctctttgttc accgtaatgt
     7921 tgctaacctg gtcattcaca ccgacctgaa ctgcctttcc gtggttcagt atgcagtgga
     7981 tgtactcgaa gttgaacaca ttattatctg tggccactac ggttgcggcg gcgtacaagc
     8041 cgccgttgaa aacccggaac tggggcttat caacaactgg ctgctgcata tccgcgatat
     8101 ctggttcaaa catagctcat tgctcggcga aatgccgcaa gagcgccgtc tggatacctt
     8161 gtgtgaactg aacgtcatgg aacaggttta taacctaggc cactccacca ttatgcaatc
     8221 agcgtggaaa cgcgggcaga aagttaccat tcacggctgg gcctacggca ttcacgacgg
     8281 cttgctgcgt gatctggatg tcacagcgac caaccgcgaa acccttgagc aacgttaccg
     8341 tcacgggatt tccaacctca agctgaaaca cgccaaccac aaataaaaat gccatgccgg
     8401 atgcaacaca tccggcaact tcacacttac tcgtcccgca gaatcacttt gccgatatac
     8461 ggcagatgac ggtaacgctg tgcgtaatca atgccgtaac ccaccacaaa ctcatccggg
     8521 atcgagaaac cgataaattc taccgggacg ttcacttcac gacgggacgg tttatccagc
     8581 agcgtacaaa tcgccagcga cttcggttcg cgcaggctta agatctcacg cactttcgac
     8641 agtgtattcc ccgagtcgat gatatcttca acaatcagca cgtccttgcc acggatatct
     8701 tcatccagat ctttgaggat tttcacatca cgggtggtgg acatgccgct accgtagctg
     8761 gaggcggtca taaagtcgac ttcatgagat acctgaactt cacggcacag gtccgccata
     8821 aacataaatg agccacgcag cagacccacc agcaccatat cgctgccgct gtctttgtaa
     8881 cgctcagtaa tctgacgacc cagttcggcg atacgcgctt taatctccgc ttcggggatc
     8941 attacttcta cagtatgttt catatctcta accatatgat ttaaaaacaa atcacttcgc
     9001 acgggcaggt tttggcaccg caacaaagcg caagacacgc agtataccag taaaactatt
     9061 cattcgcgga atctgttaat aaagctcgaa ttgtgatgac ggtcacacat gttaaaacct
     9121 ataattaatt gctactaaaa tattaatgaa ttgaaatggt gtctctttat ggcaattaac
     9181 aatacaggct cgcgacgatt actcgtcacg ctaacagccc tttttgcagc gctttgcggg
     9241 ctgtatctac tcattggcgg aggctggctg gtcgcgattg gcggctcctg gtactaccct
     9301 atcgctggcc ttgtgatgct cggcgtcgcc tggatgctgt ggtgcagtaa acgcgccgcg
     9361 ctttggctat acgcagccct gctgctcggc accatgattt ggggcgtctg ggaagttggt
     9421 ttcgacttct gggcgctgac tccgcgcagc gatattctgg tcttcttcgg catctggctg
     9481 atcctgccgt ttgtctggcg tcgcctggtc attcctgcca gcggcgcagt tgccgcactg
     9541 gtggtggcac tgctgattag cggtagtatc ctgacctggg ccggatttaa cgatccgcag
     9601 gaaatcaacg gcaccttaag cgccgatgcc acacctgcgg aagctatctc acccgttgcc
     9661 gatcaggact ggcctgccta tggtcgcaat caggaaggtc aacgcttttc acctctgaaa
     9721 caaattaacg ccgataacgt ccataatctg aaagaagcct gggtgttccg tactggcgat
     9781 gtgaagcagc cgaacgatcc gggcgaaatc accaatgaag tgacgccgat taaagtgggc
     9841 gacacccttt acctgtgtac cgctcaccag cgcctgtttg cgctcgatgc cgccagcggc
     9901 aaagagaaat ggcattacga tcctgagctg aaaactaacg agtctttcca gcacgtaacc
     9961 tgccgtggcg tctcttatca tgaagccaaa gcagaaacgg cttcgccgga agtgatggcg
    10021 gattgcccgc gtcgtatcat tcttccggtc aatgatggtc gactgattgc gattaacgct
    10081 gaaaacggca aactgtgcga aaccttcgcc aataaaggcg tgctcaatct gcaaagcaat
    10141 atgccagaca ccaaaccggg tctgtatgaa ccgacttcgc caccgattat caccgataaa
    10201 accatcgtga tggccggttc agttaccgat aacttctcaa cccgcgaaac gtctggcgtg
    10261 atccgtggtt ttgatgtcaa caccggggag ctgctgtggg cttttgatcc cggcgcgaaa
    10321 gatccgaacg caatcccgtc tgacgaacac acctttacct ttaactcgcc aaactcctgg
    10381 gcaccagcgg cctatgacgc gaagctggat ctggtctatc tgccgatggg cgtgaccacg
    10441 ccggatatct ggggcggtaa ccgcacaccg gaacaggaac gttatgccag ctcgattctg
    10501 gcgctgaatg ccactaccgg gaaactggcg tggagctacc agaccgttca ccacgacctg
    10561 tgggacatgg atcttccggc acagccgacg ctggcggaca tcaccgttaa tggtcagaaa
    10621 gtgccagtta tttacgctcc ggcgaaaacc ggcaacattt ttgtgctcga tcgtcgcaat
    10681 ggcgaactgg tggttccggc cccggaaaaa ccggtgccgc aaggtgctgc gaaaggcgat
    10741 tacgttactc cgacccagcc gttctctgaa ctgagcttcc gtccgaagaa agatttgagc
    10801 ggtgcggata tgtggggagc caccatgttt gaccaactgg tgtgccgcgt gatgttccac
    10861 cagatgcgct atgaaggcat tttcacgccg ccatctgaac agggcacgct ggtcttcccg
    10921 ggtaacctgg gaatgtttga atggggcggg atttccgttg atccaaatcg tgaagtggca
    10981 attgccaacc caatggcact gccgtttgtt tcgaaactga tcccacgcgg tccgggtaac
    11041 ccgatggagc agccgaaaga tgccaaaggc acgggtacgg aatccggcat tcagccacag
    11101 tacggtgtac cgtatggtgt cacgctcaac ccgttcctct caccgtttgg tctgccgtgt
    11161 aaacagccag catggggtta tatctcggcg ctggatctga aaatcaatga agtggtgtgg
    11221 aagaaacgta ttggtacgcc gcaagacagt atgccgttcc cgatgcctgt accggtgccg
    11281 ttcaatatgg gtatgccgat gctgggcggg ccaatctcca cggcgggtaa cgtgctgttt
    11341 atcgctgcta cggcagataa ctacctgcgc gcttacaaca tgagcaacgg tgaaaaactg
    11401 tggcagggcc gtttaccagc gggtggtcag gcgacgccaa tgacctatga agtgaatggc
    11461 aagcagtatg tggtgatctc cgcaggcggt cacggttcat ttggtacgaa gatgggcgac
    11521 tatattgtgg cttatgccct gccggatgat gtgaaataac cataatgccc ggagagatcc
    11581 gggcatattt ccgaatacgg tctttttata ccgtaaaccc taacatcatc cccgtatctt
    11641 catgctccag cagatggcag tgcgccatat aagcatgttc tttcggtgcg ttgtgattaa
    11701 acttcaccag cacttcactg acattgcctt ctaccttaac ggtatctttc cagcccgcgc
    11761 gatgagccgc tggcggtttg ccattttctg acaagatacg gaactgcatg ccgtgaatat
    11821 ggaacggatg cagcatcatg tcgcccacgc cagagataac ccaacgttcg tactgccctt
    11881 tcgccgccgc aaacatcggc ttattcatat caaacgcctg accgttgatt ttattggcat
    11941 ggtggaaatc gaacttcccg ccgtggttca tatgattcat attgccgtgc cccatatggc
    12001 ccatcatctg gctgtgatcc attccggcca tcgcctgatc gccatatttc tccattagca
    12061 tctgcatccc catcatatcg agcatcgggt ccatagaaag ttgcagcttg cgtaccgtca
    12121 gcccttccag cgaaggtaac gcaggcaggc tacttaatgt gtcaggcaaa gcaccggagg
    12181 cactaatagc aatcggctga atccgcatta ccggatgcgg cttgtcaaac ggcgcaatcg
    12241 ccatccccat ctggctgacc ggtagcgtca ccagatcaaa gggtttgttg tcgttaacct
    12301 ccaccagcac ttcaaaacgc tcgcccatca gcaccggcag ctcgctcacc ttcaccggtt
    12361 caggtagcag accaccgtcg ctggcaatca catacagcgg gcgattgtcg ctggtggcga
    12421 aattgagcga gcgggcgtta cagccattga gcaaacgcag gcgcagccag ccacgcgggg
    12481 cagcgtgttg tgggtagatt gcaccgttgg tcagcagcgt atcgccaaac cagcccacgg
    12541 cggcggtcat cacatccagt tgataatcaa tctgcccatc agcgttaaat tttttatcct
    12601 gaacaatcac cggaacatca tcgatacccc actgttttgg cagcattaat ttcaggatct
    12661 cgtcatcttc aattaccacc agcccagcca accccatcgc cacctgtcgc ccggtcttgc
    12721 catgttgatg tggatggaac cagcaggtag cggcaggttg atcaacgttc aacgtcaccg
    12781 tacgtttgcc gcctggcgga ataattccct gcgggccgcc gtcgacgtca cccggtactt
    12841 ccagcccgtg ccagtgcaac gtcgtctctt ccgccagttg gttatagata tcaaccgtta
    12901 ccgctttgcc gcgctgtaat ttcaccgccg gccccagcag attgccgtta tagccccagg
    12961 tagttgcagt tttctcgcca aaggtagact gacctgcgcc aatagttaac tgaatgcgat
    13021 tacgggcatc ggtcgtgagc aaatcaggga tcggtaacgt tgggcgttcc gccgcaaaga
    13081 ctgcgcggct ccacagcggc aaggctgaag ccacacccag cgcgacggaa tattttaaga
    13141 aatcacgacg ttgcatagtt atttccttat tcttaagcag gcgaaacaaa atcaaacgtt
    13201 gagcatagtc cttcccctta cgggaaggtc aagccgcaga cacaatttaa tcgacaataa
    13261 tggtcgtcgc ctcgctcgca gtgtgctaac gtttatcttc tttaagcccc tggtagaagc
    13321 aatgaagacg tttttcagaa cagtgttatt cggcagcctg atggccgtct gcgcaaacag
    13381 ttacgcgctc agcgagtctg aagccgaaga tatggccgat ttaacggcag tttttgtctt
    13441 tctgaagaac gattgtggtt accagaactt acctaacggg caaattcgtc gcgcactggt
    13501 ctttttcgcc cagcaaaacc agtgggatct cagtaattac gacaccttcg acatgaaagc
    13561 cctcggtgaa gacagctacc gcgatctcag cggcattggc attcccgtcg ctaaaaagtg
    13621 caaagccctg gcccgcgatt ccttaagcct gcttgcctac gtcaaataat ccctgatacc
    13681 tttttgtaga aatattgacc gtgcatccgc ggtcaatgtt agctattatg ttgcgccctt
    13741 tttttacggg tgttaacaaa ggaggtatca acccatggcc gaaaaaaaac agtggcatga
    13801 aacgctacac gaccagtttg ggcagtactt tgcggtagat aacgttctgt atcatgaaaa
    13861 gaccgatcac caagatctga tcatttttga gaacgctgca tttggtcgcg taatggcgct
    13921 ggatggcgta gtacaaacca ccgagcgcga cgagtttatc tatcatgaga tgatgaccca
    13981 tgttccgcta ctggcccacg gtcacgcgaa acatgtgctg attatcggcg gcggcgacgg
    14041 tgccatgctg cgtgaagtaa cccgacataa aaacgttgag tcaatcacga tggtggaaat
    14101 cgatgcgggt atcgtgtcgt tctgccgtca gtatctgccc aaccataacg ccggtagcta
    14161 cgacgatccg cgctttaagc tggtgatcga cgatggcgtc aatttcgtta atcaaaccag
    14221 ccagaccttt gatgtcatta tctccgactg caccgatcct atcggtcccg gcgaaagtct
    14281 gttcacctca gcattttatg aaggctgcaa acgttgcctg aatcctggcg gtatcttcgt
    14341 cgcacaaaac ggcgtctgct ttttacagca ggaagaagcc atcgacagct atcgcaaact
    14401 cagccattac ttcagcgacg ttggctttta tcaggcggcg atcccgacct attacggcgg
    14461 tatcatgact tttgcatggg cgacagataa cgacgcctta cgccatctct caaccgaaat
    14521 tattcaggcg cgttttctcg cttctggcct gaaatgccgt tattacaatc cggcaatcca
    14581 tacggcagct tttgccttac ctcagtatct gcaagacgca ctggcttcac agccgtccta
    14641 aggagaagat aagaaattga aaaaactgaa actgcatggc tttaataatc tgaccaaaag
    14701 tctgagtttt tgtatttacg atatctgcta cgccaaaact gccgaagagc gcgacggtta
    14761 tattgcttat atcgatgaac tctataatgc caaccgtctg accgaaatcc tgtcagaaac
    14821 ctgttccatt atcggggcca atattcttaa catcgcccgc caggattacg aaccacaggg
    14881 tgccagcgtc actattctgg tgagtgaaga accggttgac ccgaaactca tcgacaaaac
    14941 agaacacccc ggcccactgc cagaaacggt cgttgcccat ctcgataaaa gtcatatttg
    15001 cgtacatacc tacccggaaa gtcatcctga aggcggttta tgtaccttcc gcgccgatat
    15061 tgaagtctct acctgcggcg tgatttctcc gctgaaggcg ctgaattacc tgatccacca
    15121 gcttgagtcc gatatcgtaa ccattgatta tcgcgtgcgc ggttttaccc gcgacattaa
    15181 cggtatgaag cactttatcg accatgagat taattcgatt cagaacttta tgtctgacga
    15241 tatgaaggcg ctgtatgaca tggtggatgt gaacgtctat caggaaaata tcttccatac
    15301 caagatgttg cttaaagagt tcgaccttaa gcactacatg ttccacacca aaccggaaga
    15361 tttaaccgac agcgagcgcc aggaaattac cgctgcgctg tggaaagaaa tgcgcgagat
    15421 ttattacggg cgcaatatgc cagctgttta acggctctgg cggagctccc aggctccgcc
    15481 agatttattt acttctgctg cacgaaattg cggtaagccg ccacgacctg cagaaaatcc
    15541 tcaacgccgc atagcgacag gctttcttcg tcgtagtagt tcatcccttc ttccatttca
    15601 tcgccagcga attccagttg attggcgcga accatcacct cttcaccgtc catccacagg
    15661 gtgtattcat gccctgcccg ttgccaggac cgttcgctac ctttcagtgc gtgcgctgct
    15721 tgttccactt catcaagcaa agccaggttt tctttcacct cttcattaaa ccagtgcccg
    15781 accacttcat gccccatgga catacgcacc tttaccactc cggtaatatc gcgcagaaat
    15841 tcgtaatcca tagtgtgttc ctctgctccc ggcaatgcgc cgaaatcgca ttgcgctatt
    15901 gatgtaatta ttgcagcctt accggagaaa aacagcggaa cggcgatggt ttagaaataa
    15961 aaaatgcccg ggaatctcat tcaccgggca ttgtgtcgtt tatgcgcagc gcgtgcgctt
    16021 acttattaca ccgcagtctg gaaaatcacc ccatcggctt tttcggtgta ctgagaaagc
    16081 tggttgaagt tcagataacg ataagtatca acggcagttt tatctacttg cgcaacgtag
    16141 gtctggtact cttccggcgt cggcagtttg ccaatcagcg ccgcaacagc cgccagttct
    16201 gcagaagcca ggaagacatt cgcgccagtc cccagacggt tcgggaagtt acgggtagag
    16261 gtggaaacca ccgtcgcacc gtctgctaca cgcgcctggt tacccataca cagagaacag
    16321 cccgggatct cgatacgagc cccgctctta ccgaagacgc tgtagtagcc ttcttcagtc
    16381 aactgtgcgg cgtccatgcg ggttggcggt gccacccaca ggcgggtcgg caactggcct
    16441 ttgtgcgcat ccagcagttt acccgcagca cggaagtgac cgatgttagt catgcaagaa
    16501 ccgataaaca cttcgtcgat cttctcgccc tgtaccgcag acagcggacg tgcgtcgtcc
    16561 gggtcgttcg gtgcacagag gattggctct ttaatatccg ccagatcgat gtcgatcact
    16621 gccgcgtatt cagcatctgc atcggcttcc agcagctcag gattcgccag ccatttttcc
    16681 atgccctgaa tacgacgttc gagggtacga cgatcgccat aaccttccgc gatcatccac
    16741 ttcagcagga cgatgttaga gttcaggtat tcgatgatcg gttctttgtt cagcttgata
    16801 gtacaaccag cggcagaacg ctcagcggac gcatcggtca gttcaaatgc ctgctcaact
    16861 ttcagatccg gcagaccttc aatttccaga atgcgaccag agaagatgtt tttcttgcct
    16921 ttcttctcaa cggtcagcag accttgtttg atcgcataca gcgggatcgc gtgtaccaga
    16981 tcgcgcaggg tgatgcccgg ctgcattttg cctttgaagc gcaccagaac ggattccggc
    17041 atatccagcg gcattacgcc tgttgcggct gcaaatgcca ccagaccaga acccgccggg
    17101 aaagagatac cgatcgggaa acgggtatgg gagtcaccac cggtaccgac ggtatccggc
    17161 agcagcatac ggttcagcca ggagtggatt acgccgtcac ccggacgcag cgacacacca
    17221 ccacggttca taatgaagtc cggcagcgtg tggtgcgtgt tcacgtcaac tggcttcgga
    17281 tacgccgcgg tatggcagaa agactgcatc accaggtcag ccgagaagcc caggcacgcc
    17341 aggtctttca gttcatcacg ggtcatcggg ccggtggtgt cctgtgaacc tacagaagtc
    17401 attttcggtt cgcagtacgc gcccggacga atacctttca caccgcaggc acgacctacc
    17461 attttctgag ccagcgagaa gccgcgatcg ctctcagcga catctttcgc ctgacggaac
    17521 acatcactgt gcggcagacc aagtgcttca cgcgctttgg tggtcaggcc acgcccgata
    17581 atcagcggaa tacggccacc agcacgcact tcatcaatca gcacgtcggt tttcagttcg
    17641 aaggtcgcca gcagttcgcc ggtttcgtgg ttacgcactt cacctttgta cgggtaaacg
    17701 tcaatcacgt cgcccatgtt caggttagag acgtcgactt cgattggcag tgcacccgcg
    17761 tcttccatcg tgttaaagaa gatgggtgca attttaccgc cgaggcacaa accaccgccg
    17821 cgtttgttcg gcacatgtgg aatatcatcg cccataaacc acagaacgga gttcgtggcg
    17881 gatttacgcg atgaacccgt acccacaacg tcaccgacgt acgccagcgg gaaacctttc
    17941 tgttgcagag cttcgatttg cttgatcggg ccaacaacac ctggctggtc tggctcaata
    18001 ccttcacggg cgtttttcag catcgccagc gcgtgcagtg ggatatccgg gcgcgaccac
    18061 gcatccggtg ccggagaaag gtcatcagtg ttggtttcgc cagtgacttt gaagacggta
    18121 acggtcagtt tttcagccag cgccgggcga ttcaggaacc attcggcatc cgcccaggac
    18181 tgcattacct gcttcgcata ttcgttgcct gctttggctt tctcttctac gtcatagaag
    18241 ttatcgaaca tcagcagtgt gtgagaaagt gctttggcag cgatcggtgc cagtttggca
    18301 tcatccagcg cgtcgatcag cggatgaatg ttgtaaccac cctgcatggt acccagcagt
    18361 tcgatggctt tttccggagt cagcagaggg gatttggctt cgcctttagc gatagcagcc
    18421 aggaagcctg ctttgacata ggcggcttca tcgacgccag ggggaacacg gttggttaat
    18481 agatctaaca ggaattcttc ttcgcccgcg ggcgggtttt tcagcagctc tacaagtgcg
    18541 gccatttggt ttgcatccag gggtttgggc gcaatcccct cagcggcacg ctcagctacg
    18601 tgcttacggt attcttctag cacgacggtt ctcctcgctc tcattgtcat agtgcggcag
    18661 gcgattctct tcacgctcct gtgagacagc agtttgtagg gtaaatgccc agtaccgcaa
    18721 cgggcagaat agcaggattt tgacgacgtg ttaatctgtt tacaaaaaag caacataaaa
    18781 aagtggctga atcgttaagg atggtctaga gattgtttcc ctccgtaaaa acttcgttaa
    18841 ttcgcatggt gataatcacc tttctcaacg aatcccaaga ttagtcaaaa tttaaactac
    18901 cgcctcttta tactcggatt aacagcacct gcgggtggca gttcgccgac cattgcgatt
    18961 tccttgagat ccgaattatg aaaatgactt tgccgtttaa accccatgtg ctggcactaa
    19021 tttgcagtgc cgggctttgt gccgcctctg ccgggctata tataaaaagc cgcacagtgg
    19081 aagcgcctgt ggaaacgcaa tcgacacaac agactgcgcc tgacatcacc gcagttacgc
    19141 ttcctgcaac ggtttccgca ccgcccgtaa cgcccgccgt cgtcaaatcc gcattcagca
    19201 ctgcacaaat cgatcaatgg gttgcgcctg tcgcgctgta ccccgattct ctgctttcac
    19261 aagtgttaat ggcatcaacc tatccggcaa acgttgctca agcagtgcaa tggtcgcacg
    19321 ataatccact taaacaaggc gatgctgcta ttcaggcggt atctgaccag ccgtgggacg
    19381 ccagcgttaa atcactggtg gcctttccac aattgatggc attgatgggc gaaaacccgc
    19441 aatgggtgca aaacctgggc gatgcttttc tggcgcagcc gcaggacgtg atggactcgg
    19501 tacagcgatt gcggcaactg gcgcaacaaa ccggctcgct gaagtcatca accgaacaga
    19561 aagttattac cacaacgaag aaagctgtac cggtaaaaca gacagtcacg gcacccgtca
    19621 taccatccaa taccgtttta actgccaacc ccgtcattac agagccggca acaaccgtca
    19681 tttccattga gcccgccaat cctgatgtgg tctatattcc caactacaac ccaaccgtgg
    19741 tttacgggaa ctgggccaat actgcgtatc cgccggttta tctgccacca ccagccggag
    19801 aaccgtttgt tgacagcttt gtgcgcggat tcggctatag catgggcgtt gctaccacgt
    19861 acgcactatt tagcagcatc gactgggatg acgacgatca tgaccatcat catcatgacg
    19921 atgatgatta tcatcaccac gatggcggtc atcgtgacgg taatggctgg caacacaacg
    19981 gcgacaacat caatatcgac gtcaacaatt tcaaccgtat caccggtgag catcttactg
    20041 ataagaatat ggcatggcgg cacaatccaa actaccgtaa tggtgtgccc tatcatgatc
    20101 aggatatggc aaagcggttt catcaaaccg atgtcaacgg cggaatgagt gccacgcagc
    20161 tacctgctcc aacacgcgac agccagcgtc aggcggcagc aagtcagttt cagcaacgaa
    20221 cacacgccgc ccccgtcatt acacgagata cccaacgtca ggcagcggca cagcggttta
    20281 atgaagctga acactatggg agctatgacg acttccgcga cttcagccgt cgccaaccac
    20341 tgacccagca acaaaaggac gccgctcgtc agcgttatca atcggcctct cctgagcagc
    20401 gccaggcggt tcgcgagaaa atgcagacta acccacagaa ccagcagcga agagaggcag
    20461 cgcgtgagcg tattcagtct gcaactcccg aacagcgcca ggtgtttaag gaaaaagtac
    20521 agcagcgccc actgaaccaa cagcaacgtg ataacgcccg ccagcgtata caatcggcat
    20581 cacctgaaca acgtcaggtt tttcgggaaa aagttcagga gagccgccca caacgtctaa
    20641 acgacagtaa ccatactgtc aggctgaata acgagcaacg gtcagcagta cgcgaacgtc
    20701 tctctgagcg cggagcaagg cgacaggaaa ggtaaattgc agacgtaaaa aaagcggcgt
    20761 ggttagccgc ttttttaatt gccggatgtt ccggcaaacg aaaaattact tcttcttcgc
    20821 tttcgggttc ggcaggtcgg taatgctacc ttcgaacact tctgccgcca ggcccacaga
    20881 ctcgtgcaga gtcgggtgcg cgtggatggt cagtgcgatg tcttcagcat cacaacccat
    20941 ttcgattgcc agaccgattt cacccagcag ctcaccgccg ttagtaccga caatcgcacc
    21001 accgatcaca cggtgagatt ctttgtcgaa aatcagcttg gtcataccgt ctgcgcagtc
    21061 ggaagcgata gcacgaccag aagcagccca cgggaaggtg gcggtttcat agctgatgcc
    21121 tttctctttc gcttctttct cagtcagacc tacccatgca acttctggtt cggtataggc
    21181 gatagacggg ataactttcg gatcgaagta gtgtttttta ccggcgataa cttcagcggc
    21241 aacgtgacct tcgtgaacac ctttgtgtgc cagcatcggc tgaccaacga tatcgccgat
    21301 agcaaagatg tgcggtacgt tggtacgcag ctgtttgtca acgcggatga aaccacggtc
    21361 gtccacttcc acgccagctt tgcctgcgtc gaggttttta ccgttcggca cacgaccaat
    21421 cgctaccagc acggcgtcgt aacgctgcgg ttcagcgggt gcttttttgc cttccatcgt
    21481 cacataaata ccgtcttctt tcgcttcaac ggcggtaact ttggtttcca gcatcaggtt
    21541 gaatttcttg ctgatacgct tggtgaagac tttaacgatg tctttgtcag ctgccgggat
    21601 aacctggtcg aacatttcaa ccacgtcaat ctgtgaaccc agcgcgtggt atacggtgcc
    21661 catttccaga ccgatgatac cgccacccat taccagcagg cgttctggta cttctttcag
    21721 ttccagcgcg tcagtggagt cccagatacg cggatcttca tgcggaataa acggcagttg
    21781 gatcgggcga gaacccgctg caatgatcgc attgtcgaag ttgatcacgg ttttgccgtt
    21841 ctcaccttca acttccaggg tgttagcccc ggtgaattta cccagaccgt tgaccacttt
    21901 gactttgcgg cctttcgcca taccagccag accaccggtc agctgattga tcactttctc
    21961 tttccaagta cgaatcttgt caatatcggt tttcggttcg ccgaagacga taccgtgttc
    22021 agccagcgct ttggcttctt cgataacttt tgctacgtgc agcagtgctt tagaagggat
    22081 acagccgacg ttcaggcaaa caccgccaag ggtgttgtaa cgttctacga ttacggtttc
    22141 cagacctaaa tcagcgcaac ggaaggcagc ggagtaacct gcggggcctg ccccaagtac
    22201 cacgacctga gttttgattt cagtactcat catgacctct atatatttat ctccggcggt
    22261 catacccgtc gtctttcagg ccgcagcggc gttagcttca cgccctcacc ccagtcactt
    22321 acttatgtaa gctcctgggg attctggcgc ttgctacctt gctgcaccca gaaatccata
    22381 gggtatgtga agtaataccc taaccaccac cgggtcgttc tatccgtcgg tattttacaa
    22441 aattgttaac aatttttaaa caacaaacgg caaccgattt gtctattcgc taataacctc
    22501 aatacattca tgagattacc agaaaaaagc cggccgttgg gccggctctt ttacttacat
    22561 caccagacgg cgaatgtcag acagcgtgtt gttaatgatg gtaatgaaac gggcaccatc
    22621 agcaccgtcg atcacgcggt ggtcgaagga gagagaaatc ggcagcatca gacgcggcac
    22681 gaactcttta ccattccaca ccggctccat cgcggacttg gaaacgccga ggatagccac
    22741 ttccggcgcg ttcacaatcg gcgcgaagtg ggtagtaccc aggccgccga tgctggagat
    22801 ggtgaagcaa ccgccctgca tttcgcccgc agtcagctta ccgtcacgcg ctttcttaga
    22861 aatagtcatc agctcgcgag acagctcgat gatgcctttc ttgttgacgt ctttgaatac
    22921 cggaacaacc agaccgttcg gggtatccac cgccacaccg atgttgatgt atttcttcag
    22981 ggtcagacgc tgaccgtctt ccgacagcga actattgaag cgaggcatct gctcaagagc
    23041 tgcagcaacg gctttcatga tgaagacaac cggggtgagc ttcacatcca gcttacgttt
    23101 cgccgcttct tcgttctgct gtttacggaa cgcttccagc tcggtgatat cggttttgtc
    23161 gaagtgggta acatgcggga tcatcaccca gttacggctc aggttagcac cagaaatttt
    23221 ctggatacgg cccagttcca cttcttcgat ttcgccaaac ttgctgaagt ccaccttcgg
    23281 ccacggcagc atgccaggga taccaccgcc agtcgccgcc ggagctgctt ctgcacgttt
    23341 gatagcttct ttcacgtaag cctgaacgtc ttcgcgcagg atacgacctt tacgaccagt
    23401 gcccttcact ttcgccaggt taacgccaaa ctcgcgtgcc agacggcgga tcagcggagt
    23461 cgcgtgaacg taagcgtcgt tttcagcaaa ttcagatttg ccttcggctt tcgcagccgg
    23521 agctgctgcc ggggcttcag ctttcgctgc cggagccggc gctgccgctt cctgtttcgc
    23581 aggagctgcc gcaggcgctg cgccttcaac ttcgaagatc ataatcagcg agccagtttt
    23641 cactttatcg ccaacgttga ctttcagttc cttcacgacg cctgcaaacg gagccgggac
    23701 ctccatagaa gctttatcgc cttctacggt gataagcgat tgttcagcgg caactttgtc
    23761 gcccactttc accatcactt cggtcacttc aacttcgtca ccgccgatat ccggaacgtt
    23821 aacttctttc acgccagccg ctgatgcagg ggccgctgcc ggagctgctt cctgtttagc
    23881 cgccggagct gccgcgcctg cttcacccgc gacttcgaag accataatca gcgagccggt
    23941 agacactttg tcacccacgt tcactttgat ctctttcacg gtgccagcaa acggagccgg
    24001 aacttccata gaagctttat cgccttctac ggtgatcagc gactgttcag cttcaacttt
    24061 atcgccaact ttcaccagga tttcggtcac ttcaacttcg tcgctgccga tatccggaac
    24121 gttaacgtct tttgccgccg cagccgcggg tgctgctgcc ggagctgctt ctttcttctc
    24181 ttctgcctga gcaggtgcag cgtctgctgc accgtcggcg gaatcgaaaa tcataatcag
    24241 tgcgccggtc tgggttttat cgccaacaga gactttgatc tctttaacga tacccgcctg
    24301 cggagacgga acttccatag aggctttgtc gccttctacg gtgatcagcg actgttcggc
    24361 ttcaactttg tcgcccactt tgaccaggat ctcggtgatt tcaacttcat cagccccgat
    24421 gtccggtact ttgatttcga tagccattat tcttttacct cttacgccag acgcgggtta
    24481 actttatctg catcgatgtt gaatttggcg attgcgtcag caaccacttt cttatcgatt
    24541 tcgccacgtt tagccagttc gcccagcgcc gcaaccacga cataagaagc atcaacttcg
    24601 aagtggtgac gcaggttctc acggctgtcg gaacgaccga agccatcagt acccagtacg
    24661 cggtagtcgt cagccggtac gtaagtacgg acctgctcag cgaacagttt catatagtcg
    24721 gtagatgcca ctgccggagc gtcgttcatc acctgagcga tatacggaac gcgcggagtt
    24781 tccagcggat gaagcatgtt ccagcgttca caatcctgac catcacgcgc cagctcggtg
    24841 aaggaggtca cgctataaac gtcagaacct acgccgtaat ctttcgccag gatctctgcc
    24901 gcttcacgga cgtgacgcag gatagaaccg gagcccagca gctgaacttt acctttgcta
    24961 ccttcaatgg tttcgagttt gtagatacct ttacggatac cttcctcagc accttccggc
    25021 attgccggca tgtggtagtt ttcgttcagc gtagtgatgt agtagtaaac gttctcttgt
    25081 ttttcaccgt acatacgctc cagaccgtca tgcatgatga cagcaacttc gtaagcgtaa
    25141 gccgggtcgt aagagataca gttcgggata gtcagcgact gaatgtggct gtgaccatct
    25201 tcgtgctgca gaccttcacc gttcagggtg gtacgaccgg aagtaccgcc gatcaggaag
    25261 ccacgcgctt gctggtcgcc agccgcccag cacagatcgc cgatacgctg gaagccgaac
    25321 atcgagtaat agatgtagaa cggaatcatc ggcagattgt tggtgctgta agaggtcgcc
    25381 gctgccagcc aggaacaccc tgcgcccagc tcgttgatcc cttcctgcag aatctgacct
    25441 ttctcgtctt ctttatagta agcaacctgc tcgcggtcct gcgggatgta ctgctgaccg
    25501 ttcgggctgt aaataccaat ctgacggaac agaccttcca taccgaaagt acgcgcttcg
    25561 tcggcgatga tcggtaccag acgatctttg atcgacttgt tcttcagcat tacgttcagg
    25621 gcacgaacga aagcgatagt ggtagagatc tctttgctct gctcttccag cagcgcgccg
    25681 aagtcttgca ggctcggcag ctcaagcttc tcggtgaagt tcggctgacg gcttggcaga
    25741 taaccgtgca gtttctgacg ctgtgcgtgc agataggtat gctcttcaga accttccggg
    25801 aaggtgatgt acggcagttt ttcgatatct gcatcagaca ccggcacatt gaaacggtcg
    25861 cggatgtgac gcacgccgtc catgttcatt ttcttaacct ggtgcgcgat gtttttacct
    25921 tcagccgcgt cgcccatgcc gtaaccttta atggtatgag caaggattac tgtcgctttg
    25981 cctttggttt cctgcgcttt cttgaatgca gcgtagattt tcttcggatc gtgaccacca
    26041 cggttcagtg cccagatctg ctcgtcagtc cagtctgcaa ccagtgctgc ggtttcagga
    26101 tatttaccga agaagtgttc acgaacgtac gcaccatctt tcgatttgaa ggtctggtag
    26161 tcgccgtcaa cggtttcgtt catcagctgg atcagtttac cgctggtatc tttacgcagc
    26221 agttcatccc aacggctacc ccacatcact ttgataacgt tccagccagc accttcgaag
    26281 atgccttcca gttcgttgat gatcttgccg ttaccggtga ccgggccgtc aagacgctgc
    26341 aggttacagt tgataacgaa gaccaggtta tccagttttt cacgggtagc gatggtgatc
    26401 gcacctttgg attccggttc gtccatttca ccgtcgccga ggaacgcgta aacggtctgt
    26461 ttagaggtat ctttcaggcc acggtgttcc agatatttca ggaatttagc ctggtaaata
    26521 gcaccaatcg gccccagacc catagatacg gtcgggaact gccagaattc cggcatcagt
    26581 ttcgggtgcg gataggaaga gaggccattg ccgtgaactt cctgacggaa gttatccagc
    26641 tgctcttgag tcagacgacc ttccaggaaa gcacgagcgt acacgcccgg ggagatgtgg
    26701 ccctggaagt aaaccaggtc gccgccatcc tgctcgttgc gtgcacggaa gaagtggtta
    26761 aagcacacat cataaatggt tgcggaagac tggaaggacg ccatatggcc gcccagttcg
    26821 aggtcttttt tcgacgcacg cagcaccgtc atgatggcgt tccagcggat agctgaacga
    26881 atacggcgtt ccagttccag attacccgga tactccggtt gttcttcaac ggggatggtg
    26941 ttgatgtagt tgctgatacc tgtgcctgcg gctacgttta caccgccttt gcgggcttca
    27001 gcaagcagtt ggtcgatcag atactgagca cgctcaacac cttcttcacg gatgaccgat
    27061 tcgatcgcct ggagccagtc gcgagtttcg atcggatcca cgtcatttgg gaaacgttct
    27121 gacatgggtt attccttatc tatctaataa cgttgagttt tctggaacct gtcccattga
    27181 actctcgccg gaaagctcaa taagacaggt tctacgttta gttgccgcgc ttttatatac
    27241 gcttgattta caacatcttc tggataattt ttaccagaaa aatcactaat tctttcgttg
    27301 ctccagacga cgcagagaac gctcacggcg gctctcttca cgacttctgt cgagcaaaat
    27361 ttcttcgata aaggccagat ggcgatgcga tgcttcgcgc gcttcttccg gcttaccggc
    27421 cataatcgct tcaaatatgc gggtgcggtg actactcacc agcggcagca tctcgcgacg
    27481 cgaatagagc aattcgaagt tctggcggac attctgggcc aacatcggct ccatacacct
    27541 tagcagatga agcagaacca cattgtgggc cgcttcggtg acggcaatct gatactggag
    27601 tacggcgttt gattccgcgt ccagatcgcc agactgctgc gccagctcta tggcgtggtg
    27661 gagttcacgg atgcgttcct tgtcttcatc ggtactacgc agcgcggcgt aataagcggc
    27721 gataccttcc agggcgtgtc gtgtttcgag caagtcatac tgtgattcag gatggtcgga
    27781 gagcagctcc accagcggat cgctgaagct ttgccatagg ctgctctgga caaaagtgcc
    27841 gccaccctgg cgacgaagca acaagccctt cgcttcgaga cgttgaatcg cctcacgcaa
    27901 ggagggacgg gagacgtcga actgttttgc cagttcgcgt tccggtggga gtttttcgcc
    27961 cgggcggaga gtgccttcga gaatcaaaaa ctccagttgc tgctcaatca catcggagag
    28021 ttttggttgg cggattttgc tgtaggccat gagttcctgt cttaagccac ttgccgaagt
    28081 caattggtct taccaatttc atgtctgtga cgctaaagta acaaagtatt caccttatgt
    28141 ccatacaggt tttgattgaa atcatgaaac tgtgcacatt ttaacaactt gacatatata
    28201 acgtttcaaa attgtaacta tgcacaaatg tagactttac gtaggaaagg gattttttaa
    28261 cgattattaa actataaaaa tccgaattga accgattcac ttaccaattt tttgtttttt
    28321 aagtcaataa aaacgaattt aaattcattc tgcatattga gaggggttga ggctgagctt
    28381 tacaaacggt ttctttttaa gcaactcatc ttcaaccatg cataaagcgg gtgcattcgc
    28441 tgccgcatac cattattctt gatctgacgg aagtcttttt gtaacaattc aaacttcttt
    28501 gatgtaaaaa aattaataca acaaacggaa ttgcaaactt acacacgcat cactgcgtag
    28561 atcaaaaaaa caatcaccgc acgaggtttc atgatggaag gtcaacagca cggcgagcag
    28621 ctaaagcgcg gccttaaaaa ccgccatatt cagcttatcg cgctgggtgg cgcgataggg
    28681 acagggctat tcctgggtag cgcctccgta atacagtccg cagggccagg gattatcctg
    28741 ggttacgcca ttgctggttt tatcgccttt ctgatcatgc gtcagctggg tgaaatggtg
    28801 gtcgaagaac ctgtcgcagg ctcctttagc cactttgctt ataaatactg gggcagtttt
    28861 gccggtttcg cctctggctg gaactactgg gtactgtacg ttttagttgc catggcagag
    28921 ctgactgccg tgggtaaata cattcagttc tggtatccgg aaatcccaac ctgggtttct
    28981 gccgccgtat tctttgtggt gattaacgcc atcaacctga ccaacgttaa agtgtttggc
    29041 gagatggagt tctggtttgc cattatcaaa gttattgcga tagtagcgat gatcatcttc
    29101 ggcggctggc tgctgttcag tggcaacggc ggcccgcagg cgagcgttag caacctgtgg
    29161 gatcaaggtg gtttcctgcc gcacggcttc accggactgg tgatgatgat ggcgattatc
    29221 atgttctcgt tcggtggtct ggaactggtg gggatcaccg cagcagaagc tgataacccg
    29281 gagcaaagta tcccgaaagc gactaaccag gttatctacc gcatcctgat tttctatatt
    29341 ggttcgttag ccgttctgct ctcactgatg ccgtggaccc gcgttaccgc cgataccagt
    29401 ccgtttgtgc tgatcttcca cgagttaggc gatacctttg tggcgaatgc gctgaacatc
    29461 gtggtactga ctgcggcgct ctccgtgtac aacagctgcg tatattgcaa cagccgtatg
    29521 ctgtttggtc tggcacaaca gggtaatgcg ccaaaagcgc tggcgtctgt cgataaacgc
    29581 ggcgtaccgg ttaacaccat tctggtgtct gcgctggtta cagcattgtg cgtattgatt
    29641 aactatcttg ctccggaatc cgcatttggc ctgttaatgg cactggtggt atccgcactg
    29701 gtgatcaact gggcgatgat cagtctggcg catatgaagt tccgtcgcgc caagcaggaa
    29761 caaggcgtgg taactcgctt ccctgctctg ctttatccgc tgggtaactg gatctgcctg
    29821 ctgtttatgg cggcggtact ggtgattatg ctgatgaccc caggaatggc gatttcggta
    29881 tacctgatcc cggtatggct gatcgtgtta ggtatcggct atctgtttaa agagaaaacc
    29941 gccaaagccg taaaagcgca ttaatctctc tacgccctca cccatgcagg gtgagggcaa
    30001 taatctttac accaacgctc cgtaaatcgt cagtagcgca atcaccacca cgaccacgaa
    30061 cgaggttttc ttcgccattg aaaccgctgc cttcggcgtc tccaccttat cgacatgcgg
    30121 ttcacgcgcc agagagaact gcgccagacg cgttaacacc tgatactgcg aagtatggaa
    30181 gtcacccagc gaagcaaacc aggccggtaa cgctttctcc ccatgaccga tcaaggcata
    30241 taccacaccc gcaagacgaa ccggcaccca atccagtaca tgaagcacgg catcaatgcc
    30301 ggactgtaaa cgatgatgcg gcgtctggta tcgcgccagc cagtattgcc atgcacgcaa
    30361 aaacgcatac cccatcagcg taacgggtcc ccaggttccc cccacaatca accagaacag
    30421 cggtgcaaga taaaaacgaa agttaatcca cagcaatgca ttttgcagct cacgcaaata
    30481 ctcacgttcg tcgcagcctg ccgggacgcc gtgaatcatg gtgagttcgc cagccatcgt
    30541 ggcacgggca tggctatcat tacgtgaagc agctgtcaga taagcatgat aatgaagacg
    30601 aactttacct gcgccaatac acagcaaacc aatcagcagc cacaccagca gcgtgggaac
    30661 gttgaacaat actccctgca atgcgcgtaa cagtaaaaaa gtcacgccca tcgcaataat
    30721 ggtcatgcct aacgtgcgcc cgagagaaaa atgtttcacc cgccgaaaga acgcttcaag
    30781 acgatgatca agctgccagt gctcgcccaa cttaaacagg cgctcgaaaa ttaacaccag
    30841 taaggttgta aatagcgtca tgttgtctcc ttgctgacca gcgcacgaaa ccgtgcccaa
    30901 tcaaatgcag gaccgggatc ggttttccga tccggcgcaa tatcacaatg gcccgtcatg
    30961 ttattagcga tatcgggata gcgatctatc agtgcccgcg taaccgccgc aagctgttga
    31021 tactgcgcat cggtatacgc cagagtatcg gtgccttcaa gctcaatccc aatagaaaaa
    31081 tcattgcagc gttcacgccc ctgatactga gagactcccg catgccaggc acgtttatcg
    31141 aaaggaacat actggactat ttcaccatca cggcgaatca aacagtgagc ggagacgcgc
    31201 aaatgggcga tctcagcaaa gaaaggatgt gcctgcggat caatagttcc agtgaataat
    31261 gcgtcgatcc acggaccgcc aaactcgcct ggcggcaggc taatattgtg caccaccagc
    31321 agggtgggtg tttcgtcatc cgggcggcaa tcgtaatgtg gtgagggaac gcggcgcgcg
    31381 ccaaccagcc acccctgttc taacaacatg caggatctcc ttatatgtgg tgctaatacc
    31441 cggttcagag tagcatgttt ctaccttatg attcgttagc tatctggagt tttaacatgc
    31501 cgcctcgccg ctataaccct gacacccgac gtgacgagct gctggaacgc attaatctcg
    31561 atatccccgg cgcggtggcc caggcgctgc gggaagattt aggcggaaca gtcgatgcca
    31621 acaatgacat tacggcaaaa cttttaccgg aaaactctcg ctctcatgcc acggtgagca
    31681 cccgcgagaa tggcgtattt tgcggcaaac gctgggttga agaggtgttt attcaactgg
    31741 caggcgacga tgtcaccata atctggcatg tggatgacgg cgatgtcatc aacgccaatc
    31801 aacccttgtt cgaacttgaa ggcccatccc gcgtgctgtt aacgggcgaa cgcactgcgc
    31861 ttaattttgt gcaaaccctt tcaggagttg ccagtaaggt acgccactat gtcgaattac
    31921 tggaaggcac caacacgcag ttgttggata cgcgcaaaac cttacctggc ctgcgttcag
    31981 ctctgaaata cgcggtactt tgcggcggcg gagcgaatca ccgtctgggg ctttctgatg
    32041 ccttcctgat caaagaaaat catattattg cctccggctc agtgcgccag gccgtcgaaa
    32101 aagcgtcctg gctgcacccg gatgcgccag tagaagtcga agtagagaat ctggaagaac
    32161 ttgatgaagc cctgaaagca ggagccgata tcatcatgct ggataacttc gaaacagaac
    32221 agatgcgcga agccgtcaaa cgcaccaacg gcaaggcgct actggaagtg tctggcaacg
    32281 tcactgacaa aacactgcgc gaatttgccg aaacgggcgt ggactttatc tccgtcggtg
    32341 cgctaactaa acacgtacaa gcactcgacc tttcaatgcg ttttcgctaa ttgctgacag
    32401 atttatccgc tccatcgcgg gcggataatc tccccttccc caactttctt cgtaacgcct
    32461 cgcaaatttc ttgtcttttg ctgcaacgaa ataaaaaacc tcggaagctg ctttcccgtt
    32521 tcgttttttg cccctcgctt gtgctgatgg cgtttgccaa agtagcgcca accaaatcaa
    32581 ggagcgaaac agatggacaa gcaacgcggt tttacactta tcgaactgat ggtggttatt
    32641 ggcatcattg ccattttaag cgccattggt attcccgctt atcaaaacta cctgcgcaaa
    32701 gctgcactca ccgacatgct acaaaccttt gtgccttacc gtacagccgt agagttgtgc
    32761 gcgctggaac atggtggatt agatacctgc gacggtggca gcaatggcat tccctcgcct
    32821 accaccaccc gctatgtttc agccatgagt gtggcaaaag gcgtggtgtc gctgaccggg
    32881 caagaaagtc tcaatggact aagcgtcgtc atgacaccgg gttgggataa cgcaaacggc
    32941 gtcaccggct gggcgcgcaa ctgcaatatt caaagtgaca gcgcattgca gcaagcctgc
    33001 gaagatgtct tccgctttga tgacgccaac taaggagcgg caatgaatat tccacagctc
    33061 accgccctgt gtctgcgtta tcagggagtc ttgctggatg ccagcgaaga ggtggttcat
    33121 gttgcggtag tcgatgcacc ttcgcatgag ttgctggacg cattgcattt cgctaccacc
    33181 aaacgtattg agatcacctg ctggacgcgc caacaaatgg aaggccacgc cagtcgcaca
    33241 caacagacat tgcccgtagc tgttcaggag aagcatcagc ccaaagcaga gttgctgact
    33301 cgaacgttac aatctgcgct ggaacaacgt gcgtctgata ttcatatcga accagcggac
    33361 aatgcctacc gcatccgctt gcgtatcgac ggcgtattgc atcctttacc ggatgtttca
    33421 ccggatgccg gagtcgcatt aaccgccaga ttaaaagtgc tgggaaacct ggatattgcg
    33481 gaacatcgcc tgccgcagga cgggcaattc actgtcgaac tggcaggaaa cgccgtctca
    33541 tttcgtattg cgaccttacc atgtcggggt ggtgaaaagg tggtattaag gttgttacag
    33601 caggtgaacc aggcactgga tgttaacacg ctgggaatgc agccgttaca actggcggac
    33661 tttgctcatg ccttgcaaca accacaggga ctggtgctgg taactggccc tacaggcagc
    33721 ggcaaaacgg tcacgcttta tagtgccctg caaaagctga ataccgctga cattaatatt
    33781 tgtagcgtcg aagatccggt tgagatcccc atagccggac taaaccagac gcaaatccat
    33841 ccgcgtgccg ggctcacctt tcagggcgtt ttgcgtgcgt tattgcgcca ggatcctgac
    33901 gtcatcatga tcggagagat ccgctatggc tttaacggca gaaattgcca ttaaagccgc
    33961 gcaaaccggt cacctggtgt tgtctaccct acacactaat tccacctgcg aaacgctggt
    34021 acgtttacag caaatgggag tcgcccgctg gatgctctca tcagcgctta cgctggtaat
    34081 agcccagcgt ctggtacgta aactttgccc acattgtcgc cagcagcaag gggagcccat
    34141 ccatattcca gtcaatgtat ggccgtcgcc gctgccccac tggcaggcac ccggttgtgt
    34201 acattgctac cacggttttt atggtcgtac ggccttattt gaagttctgc ccataacgcc
    34261 ggtcattcgt cagcttattt ccgctaatac cgacgttgaa tcgctggaaa cgcacgcacg
    34321 acaggcgggt atgcgtacgc tttttgaaaa cggctgcctg gccgtggagc aaggcttaac
    34381 cacctttgaa gagttaatcc gcgtactggg gatgccgcat ggcgagtaag caactctggc
    34441 gctggcatgg cataaccggc gacggcaatg cgcaagatgg gatgctatga gcagagagcc
    34501 gtgctttgct gctcatggca ctacagcaac agatggttac cccacttagc ctgaagcgaa
    34561 tcgccatcaa ttctgcgcag tggcgaggag ataaaagcgc ggaagtcatt catcaactgg
    34621 cgacgctact caaagccggg ttaacgcttt ctgaagggct ggcactgctg gcggaacagc
    34681 atcccagtaa gcaatggcaa gcgttgctgc aatcgctggc gcacgatctc gaacagggca
    34741 ttgctttttc caatgcctta ttaccctggt cagaggtatt tccgccactc tatcaggcga
    34801 tgatccgcac gggtgaactg accggtaagc tggatgaatg ctgctttgaa ctggcgcgtc
    34861 agcaaaaagc ccagcgtcag ttgaccgaca aagtgaaatc agcgttacgt tatcccatca
    34921 tcattttagc gatggcaatc atggtggttg tggcaatgct gcattttgtt ctgccggagt
    34981 ttgccgctat ctataagacc ttcaacaccc cactaccggc actaacgcag gggatcatga
    35041 cgctggcaga ctttagtggc gaatggagct ggctgctggt gttgttcggc tttctgctgg
    35101 cgatagccaa taagttgctg atgcgccgac cgacctggct tatagtgcgg cagaaattgc
    35161 tgttacgcat cccgattatg ggttcactga tgcggggaca aaaactcacg cagatcttta
    35221 cgattctggc gctgacacaa agtgcaggca ttactttttt gcagggcgta gagagcgtca
    35281 gagaaacaat gcgctgcccg tactgggtgc aacttctgac acaaatccag cacgatatca
    35341 gtaacggtca tcccatctgg ctggcgctaa aaaataccgg ggagtttagc ccgctctgtt
    35401 tgcaattagt gagaacagga gaggcatccg gctcgctgga cctcatgtta gacaacctcg
    35461 cccatcatca tcgggataac acaatggcgc tggcggataa cctcgcagcc ttactggaac
    35521 cgacgttgct gatcataacg ggaggaatta tcggtacgct ggtggtggcg atgtatctgc
    35581 caattttcca tttaggcgat gcgatgagtg ggatgggata acgctggcgt gttgctccac
    35641 accagcgttg gaagattaca ggttgttgaa gatgcggttt tcttgttcct gcacacggat
    35701 aaatgtggtg cgcttggtca gttctttcag gcgtgaagcc ccaacgtatg tacaagctga
    35761 acgcaggccg cccaaaatat cacgcgcggt attttcaacc gggcctcgca gcggcagctt
    35821 aacggtttta ccttctgctg cgcgatattc cgcaacgccg ccaacgtgac gtttcatcgc
    35881 agactcggag ctcatgccgt agaacagcat aaatttctcg ccgttctcct caacgatgcg
    35941 accgccgctc tcttcgtggc ccgccagcat gccgccaagc atgacgaaat cggcaccgcc
    36001 gccaaaggct tttgcgacat cgcccggcgt ggtgcagcca ccgtcgctga cgatcattcc
    36061 gcccagaccg tgcgccgcat cggcacattc gattaccgca gaaagctgcg gataaccgac
    36121 gcctgttttg acgcgagttg tacaaacaga acctgggcca atgccaactt taacgatatc
    36181 agcaccggag aggataagct cctcacacat ttcaccagtc actacgttgc cagcacaaat
    36241 ggttttggtc ggccacgctt cacgcgcttt cgcaacgaac tgcacgaagt gttcggaata
    36301 accattcgcc acatcaatac aaacgaagtt taatgccggg ttcaggtcga gaatctgttt
    36361 agttttttcg aaatccgcat cagacgtacc ggtagaaacc atcacatgtt tcagcacatc
    36421 agcggaagaa ttgttgataa acgcttgcca ctcttcgaca gaatagtgtt tatgcacagc
    36481 agtcaaaata tcaaaagaag ccagcgcaga ggccatagaa aatgtaccta cggtgtccat
    36541 atttgcggcg ataatcggca cgccggacca gctctgacct gaatgtttga aggtgaattg
    36601 acgttccagt tcaacatcgg aacggctttt aagagtggag cgtttagggc ggatgagaac
    36661 gtctttaaaa cctaacttca gatcttcttc aatacgcatg tgcggattcc tggggttaat
    36721 ggctataagg gtaagagcgc aactccagtg acgctatcat acgcactaat caatgcggcg
    36781 caagactgcg aaattgtctt tttttacgct acaatcccat aaatttacct ggtaaatagt
    36841 gagcgaacgt ttcatcaatt tcgctttcgt gcactcattt ttaacgtttt gataatcatg
    36901 atgaattccc gggaatataa attatgaggt atatagttgc cttaacggga ggcattggca
    36961 gtggcaagag taccgttgcc aatgcgtttg ctgatctcgg aattaacgtc attgatgccg
    37021 atattattgc gcgtcaggtg gttgaaccag gtgcacctgc gctacatgcc attgctgatc
    37081 actttggcgc taacatgatt gctgctgatg gaacattgca gcgccgggcc ttgcgcgagc
    37141 ggatcttcgc caacccggaa gagaaaaact ggcttaacgc cctgctgcat ccgctgattc
    37201 agcaagagac gcaacaccag atccagcaag ctacttcccc ctatgtactg tgggttgtgc
    37261 cattgctggt agaaaactca ctgtataaaa aagcgaatcg agtgctggtg gtggatgtca
    37321 gcccagaaac gcaacttaag cgcaccatgc agcgcgatga tgtaactcgc gagcatgtcg
    37381 aacaaatcct tgctgctcag gcaacgcgcg aagcccgcct tgccgtggca gatgacgtca
    37441 ttgataataa cggcgcaccg gatgctatcg catcggatgt tgcccgcctg cacgcacact
    37501 atttgcagct tgcgtcgcag tttgtctcac aggaaaaacc gtaatgcaga cccaggtcct
    37561 ttttgaacat ccactaaatg aaaaaatgcg tacatggctg cgcattgagt ttttgattca
    37621 gcaactcacc gttaatttac ccatcgttga ccacgctggc gcgctgcatt tcttccgtaa
    37681 tgtcagtgaa ttactggatg ttttcgagcg cggcgaagtc cgcactgagc tgttgaaaga
    37741 acttgaccgg cagcaacgta aactccagac ctggattggc gtgcctggcg tggaccagag
    37801 ccgtattgaa gcattaattc agcagttaaa agcggcgggg agcgtattaa tttccgcgcc
    37861 gcgtatcggg caatttctgc gtgaagatcg tttgattgct ctggtgcgtc agcgactgag
    37921 catcccaggc ggctgttgca gctttgattt acctacattg cacatttggc tgcatctacc
    37981 ccaggcgcag cgcgacagcc aggtagaaac ctggattgcc agcctgaacc cgctcaccca
    38041 ggcacttacc atggtgctgg atttaattcg ccagtcggcc cccttccgta aacaaaccag
    38101 tctgaatggt ttttatcagg ataacggtgg cgatgccgac ttgctgcgcc tgaatctgtc
    38161 gctcgattca cagctttatc cgcaaatttc cggacataag agccgttttg ccattcgttt
    38221 tatgccgctg gacactgaaa acggacaggt accggaacgt ctggatttcg aactggcctg
    38281 ttgctaagga gtaaaaatgt cagaaactat tacggtgaat tgcccaacct gcgggaaaac
    38341 ggtggtgtgg ggtgaaatca gcccgtttcg gccattttgc tccaaacgtt gtcagctgat
    38401 cgacctcgga gaatgggctg ctgaagaaaa acgaatcccc agcagtggcg atctttccga
    38461 aagcgatgac tggagcgaag agccaaagca gtgacatttg tctgatgccg cacgtaggcc
    38521 tgataagacg cggacagcgt cgcatcaggc atcttgtgca aatgtcggat gcggcgtgaa
    38581 cgccttatcc gacctacaga cgcttaagct tcgcaattac cggttcattg gctggcggaa
    38641 aatcatcggc attaagaccg actagcgaca tccactcacc gggttgccct tctttacccc
    38701 acggctcccc ttcccagctt tcgaccagcc agaaccacag agttatatgc ctgtccggga
    38761 attcatattc cagtttttca aatagcgaaa aatgttgtgg ggtaatcccg acttcttcct
    38821 gaagttcacg cactaccgcc tgttccggcg tttcgcccat ttcaatttta ccgccgggaa
    38881 actccagttt attcgccatg tgcgcatctg ctgcgcgacg cgttataaag atttcattgt
    38941 tctcgttgcg aataatacct accgcaattt gcagcttttt cattgtctta aacctataaa
    39001 aaaggcgcag aatcctgcgc cttttacttc aactgttagc ttttattgca ggcgaccatg
    39061 gcactgcttg tattttttac cagaaccgca cgggcaagga tcgttacgtc ctactttgcg
    39121 ctcaccggtt tgcgccgcca gtgcagctgc ggctgcagag tcgtcatcct gatggctaag
    39181 ctgctgcatt tgcgctaaac gctcggcttc catacgacgc tgttgttcca gctcctcaac
    39241 ctcttcaggc atacgtacct ggactttgct cagcgtgctg ataacttcat attttaacga
    39301 ctccagcatc gctgcaaaca tggagaacga ttcacgtttg tattcctgct tcggatcttt
    39361 ctgtgcatag ccacgcaggt ggataccctg acgcagatag tccatcgccg ctaggtgctc
    39421 tttccacaga gagtcgagag tttgcagcat gacgcctttt tcgaagtgac gcatcatctc
    39481 agcaccaacc acttcttctt tacgctgata cacttcgatg gactgcgcca gaattcgctc
    39541 acgcagcgtc tcttcatgca gttctggttc tttatccagc cactcggcaa ttggcaaatc
    39601 gaggtcgaaa tcgttcttca gacgttcctg cagccccgga atatcccaca tttcttccag
    39661 tgactgtggc ggaatgtagg catcaatggt cgctttgaac acatcttcac gaatgctgtt
    39721 gatggtttcg ctcacatcgc tgacatccag cagttcgtta cgctgggagt aaatggcgcg
    39781 acgctgatcg ttagccacgt catcatattc cagcagttgc ttacgaatgt cgaagttacg
    39841 gctttcaact ttacgctggg cgttggcaat cgctttagtc acccacgggt gttcaatggc
    39901 ttcgcctggc ttcataccca gtttacgcat catgccggat actcggtcgg aagcaaaaat
    39961 acgcatcagc gcatcttcca tcgacaggta gaaacgggaa gaaccagcat ccccctgacg
    40021 accagaacga ccgcgcaact ggttatcgat acgacgggat tcgtgacgct cggtaccgat
    40081 gatatgcagg ccacctgctt ccagtaccgc atcgtgacgt acctgccagt cggctttaat
    40141 tttttcaatt tgctctgcgg tcggattttc cagcgcggca acttctgcct gccagctacc
    40201 accgagcaca atatctgtac cacgacccgc catattggtc gcgatagtca ccgcagccgg
    40261 ataacctgcc tgagcaacaa tcgccgcttc gttggcgtgg aatttggcgt tcaggacgtt
    40321 gtgcttaata ccggctttgg tcagttcgtt tgacaccagc tccgattttt cgatggagat
    40381 agtacccacc agcaccggct ggcctttcgc agtacgttct ttgatatctt caatgatcgc
    40441 ctgaattttt tccgcttcag tcatgtagac caggtccggc agatctttac gaatcattgg
    40501 acggttggtc ggaacaacga cggtatccag cttgtagatg gagctaaatt cgaaagcttc
    40561 ggtatcagca gtaccggtca tccccgccag tttttcatac agacggaagt agttctggaa
    40621 ggtgatcgaa gccagcgttt ggttttcgtt ctggatctgc acaccttctt tcgcttccac
    40681 agcctggtgc agaccatcgg accagcgacg gccctgcatg gtacgaccgg tgtgttcgtc
    40741 aacgatgata acttcaccat ctttaacgat gtagtcgacg tcgcgggtaa acagcgcatg
    40801 agcgcgcagc gccgccgtta cgtggtgcat cagcatgatg ttggccggag agtacagaga
    40861 ctccccttca tccatgatgc cctctttcac cagcagttct tcaatcagca ccagaccacg
    40921 ttcggtcagg ttcacctggc gagatttttc gtccaccgag aagtggcctt cgccctggaa
    40981 ggtttcggag tcttcttttt cctgacggat caggtgcgga ataattttat tcacgcgttt
    41041 atacatttcc gagctgtctt ctgccgggcc ggaaatgatc agcggtgtac gcgcttcatc
    41101 gatcaggatg gagtccactt cgtccaccag cgcatagtgc agtttacgct gtacacgttc
    41161 ttcagggctg aacgccatat tgtcgcgcag gtagtcaaag ccgtattcgt tgttcgtacc
    41221 gtaagtgatg tcagctgcgt aagcttcgcg ctttgccggt gctggcatgc ccggcaggtt
    41281 gataccgaca gtcaggccaa ggaattcaaa cagcggacgg ttgttttcgg cgtcacgttg
    41341 cgccaggtag tcgttgacgg taactacgtg cacgccttta ccggttagtg cgttcaggta
    41401 agcaggcagc gttgcggtca gggttttacc ttcaccggta cgcatttcgg cgatgcagcg
    41461 ttcgttaaga accataccgc cgagtaactg aacgtcgaag tgacgcatac caaagacgcg
    41521 cttacttgcc tcacgtacca cggcgaaagc ttccgggatc agattttcca gcacttcgcc
    41581 tttttccaga cgtgcacgaa actctgcggt tttccctttc agttcttcgt cggagagttt
    41641 ttccatctcc ggttccatgg cattgatgat gttgaccact ttgcgcatcc ggcgcagggt
    41701 gcgatcgtta cgactaccga aaactttagt taacaatttg attagcataa taaaatctca
    41761 aacgccccgc gttgcggagt taataaaatg aagtaaaggt ttattgttgt taggtgaggc
    41821 gttgagggcc agcacggatg ccttgcgcct ggcttatcca gacgggcgtg ctgaattttg
    41881 cttgtggggt aaaatgcgca taatcaatgc gataaccctt ttcagacggc gtgccttcct
    41941 gggtcagcag cgcgctgagc gtatccagta atgcaagatg ttgcgcctga agaggcaaag
    42001 actcttcagc aacgggcagt gtttgcggtg ccattgcgaa agaaagatga cggattaccg
    42061 tgcgaatggc atgttgatgc cagtaatcaa cggaatagtt cgaattcggg cggcgtgtgt
    42121 tcgcttccag caaggccaat tgaccaaagt taactttggc tgaaggctcg tggttgcggg
    42181 ttgtcgcttt tgcgggcgcg tttggttcgg cggcgttgct gagcgcaggc aaacctaaac
    42241 tcgccgcaac catccctaat aagagatgcg gccagaagta gcgtttacca aactgtcgcc
    42301 agcgcgtcag tattccactc acgttattgc catcccgatc gtattcaaac gtatttagcg
    42361 cacaaatctt atcattaaag cctgcagtcc acagcacgaa tgaagataag ttgtgcggaa
    42421 aagtgcttaa gaaaacagcg ttcgcaccgt tttttatagc tcttataaaa ttcaaaaaaa
    42481 aatgactggt tcacctggcc ggagagagtg ccagatttgc cagtcgaatt ttatacgaca
    42541 gtataaatgt cgttatgcca gtacagctga aggcgctttg aaggccaacg gcagttctgc
    42601 gtcgtcctgg aaggtcacat attcccaggc ttcctgtttc gccaggacag cctgcagcag
    42661 tttgttattc agtgcatgac cggatttata agcggtaaat gcaccaataa tattgtgacc
    42721 acacatgaac aagtcaccga tcgcatcgag cattttgtga cgcacaaatt cgtcttcaaa
    42781 acgcaggccg tcttcgttca gtacgcgata atcgtcaaca acgatggcac aatcgaagct
    42841 gccgcccagg cacaaaccac gggactgcag atattcgata tcacgcatga aaccgaacgt
    42901 acgcgcacgg ctgatctggc gcataaacgc atcagcggag aagttcatcg catagcgctg
    42961 gttgctggaa tcaatagccg gatggttaaa atcgatggtg aaatccagcg aaaaaccatt
    43021 gtacggctta aattcagccc acttatcgcc atcttcgaca cgaacagtct ctttgatgcg
    43081 aacaaatttt ttggcgcagt tcaactcgtc gataccggcg tcaagcagca ggtatacaaa
    43141 cggagcggcg ctgccgtcca tgatcgggat ttccggcgcg ttaacttcga taacaatgtt
    43201 atcgatgccc aggcccgcga gagcagcatt gaggtgctct acggttgaaa tccgtacatc
    43261 atgctcgttg accagacacg tacagagcat ggtatcacgc acagatttgg catcggccgg
    43321 gaaatctacc ggtggattca agtcggtgcg acgatagatg accccggtgt tggccggcgc
    43381 agggcgtaac gtcagagtga ctttcttgcc ggtatgtaaa ccgacacccg tcgcctgaac
    43441 gatacgttta agtgtccttt gtttgatcat cgtattatct cgccaaatta cctatccaac
    43501 cgaagtgtac tatacattcg gcgggccagt ttagcacaaa gagcctcgaa acccaaattc
    43561 cagtcaattc ttaatcagct tgcttacgca ggaatgctgg gatatccaga taatccggct
    43621 ctttcgcagt ttgcggcgca ttgtcattca cgactttagc aaccggcttc tgctcctggg
    43681 tcagcggagc catcccatgc tgctggtagc gatccatcac tggctgctga acctgcttat
    43741 tggtcaccag agtgatttca ggacgtttgt ccatgccgat acctgtcgca acaacggtta
    43801 cgcgcagctc gtcattcata tccgggtcaa gagaagtacc gataaccaca gtcgcgttgt
    43861 cggaagcaaa tgcacggatg gtgttaccta ccgtttcgaa ctcatccaga cgcaggtcga
    43921 agcccgccgt gatgttaacc agcacgccgc gcgcgccaga caggtcgata tcttccagca
    43981 gcggagaaga gatagccatt tcagcagctt cttccgcacg gtcttcaccg ctcgccacgc
    44041 cagaacccat cattgcgtag cccatctcag acattacggt gcgtacgtct gcaaagtcca
    44101 cgttcatcaa acccggacga gtaatcagtt cagcgatacc ttgcacagcg cctttcagta
    44161 catcgttcgc tgcgccaaac gcatccagca gggagatacc gcggcccaga actttcagca
    44221 gtttgtcgtt cgggatagtg atcagagagt ccacatgctt ggacagttca gtgatcccct
    44281 gctccgcgaa tgccatacgc ttcttgcctt caaagttgaa aggcttagtg acgacagcaa
    44341 cggtcaggat acccaaatct tttgccactt cagcgacgac tggtgctgca cctgtaccgg
    44401 taccaccacc catacccgca gcaataaaga ccatgtctgc accttccagc gccgcacgca
    44461 atgcatcgcg atcctcatca gccgcattgc gaccaacttc tggattagcg ccagcgccca
    44521 gtcctttggt gataccgcta ccgatttgaa tcgtctgtcc aaccgctgtt ttacgcagcg
    44581 cttgtgcatc ggtatttacc gcgaagaatt caacaccttc aatacgctcg cgcaccatgt
    44641 gttcaacagc attaccgccg ccgccgccga cgccgatgac tttaatcacc gcgtcattgg
    44701 taagttccat tggttcaaac atagtttctc tccgatttgt gcctgtcgcc tgaggccgta
    44761 atcatcgtcg gcctcataaa aattaaaact cttttcgcag ccaactattg agtcgcttga
    44821 tccacgagcc aactgatgct gtaacacgtt tttctacttc agcttcaccg ttaagatgtg
    44881 actctttccc atagtgaagc aatcccaccg ccgtcgaata atacggctcc tgagcataat
    44941 ccgttaaacc ggtaatgttc agcggcgcgc cgatacgcac ttgcgtatga aacacgcgct
    45001 gagcacaggc tgcaagacct tcgatctgcg ctgcgccacc ggttaataca atgcctgccg
    45061 ccaggtgatg tttaacccct tgttggcgaa gcttttcctg caactgcaat atctcttcgt
    45121 tgaccaggtt gagcagctcg gtatagcgcg gctcgatcac ctctgccagg gtctgacgtt
    45181 gcagactccg tggcggacga ccgcctacgc tcggcacttc cacgctctca tcttttccaa
    45241 cgatggaacc cagcgcacaa ccgtggcgaa ctttaatcgc ttcggcgtcg cttggcggcg
    45301 tgccaaaggc gtaagcgata tcactggtca cgacattgcc agcataagga attaccttag
    45361 tgtggcgcaa tgccccaccg gtataaacgg cgatatccat tgtaccacca ccgatatcga
    45421 cgacgcagac acccagttca cgttcatctt ccgtcaatac cgaataactt gatgccagtc
    45481 cggcaaatat cagttggtca actttcagcc cacaacgttc aaccgctttg acgatgtttt
    45541 tcgccatatc gttgtgacat gtgatcaggt gcacttttgc ctgcatccgc acgcccgaaa
    45601 gtcctaccgg attcttgatc ccttcctgat agtcaatcgc atactcttgc gggatcacat
    45661 gcagcacacg atgttcatcg cgcacacgca ccgatttcgc ggtatggacg acgttttcca
    45721 catcttcttg cgtcacttct tcttcagaaa taggcaccat accaatttca ttctggcagc
    45781 tgatgtgctt accagaaagc gccagatata ccgaagagat ctgacaatct gccatcaatt
    45841 ctgcctggtc aatggcgcgt tgtacgcact tgaccacgga ttcgaggtcg ttcaccccgc
    45901 ctttatccat accacgtgac gggcagctgc ccacgccaat gatattgacc ataccgtcgg
    45961 gcagaacttc ccctactaaa gcggcaacct tcgcggtacc aatctccagt cctactacca
    46021 gttttctgtc cgtcgccttg atcattgttg ttctgcctgt gcctgatttt gttgctgagt
    46081 agattcctct ggcggcaagg gcgcccagcc tactgccgct ccagagtcat aacgcaaatc
    46141 aacgtagcta atccgtttgc catcggtttg cgcctgctgc tgtaaaaccg gataaagttc
    46201 tacaaagcga gccaaacgtt tcatcgtatc gccccggcca agattgagct taatatcgtt
    46261 attcagcgtc aactgccagg aacgccgcgc ggtcatcgcc gcttccttca gagtaaatct
    46321 gtcctttgcc agcatctgcc ccatttcgcg atagccctgc aacacttcat tggcgctgcc
    46381 ttccgggcca tacagcattg gaagcacctg cttgctggtg cgatctggcg gcacgctgaa
    46441 ggtgtttcct tccgcgtcta ccatatgttg atcattccac cgcgcaatcg gcacatattc
    46501 aaccagatga atcttcaatt catcaggcca ctgctttctg acgctcacct gcttaatcca
    46561 cggcaggcgt tgttctattt gcgtctggat gatgttgaca tcctgggtca taaaggtacc
    46621 cggctcaccc aatgccagga tcgactgccg gatatcgtca ttacgcgtgt aatggcgttc
    46681 accggtcaac accagctttg agagcggcag gcgttgcgca tcttccatcc agcccaacac
    46741 gacccagccg ctcaccaaca ctgtcgttaa aacggtcagc aggaaaagga tccccgccag
    46801 acgcgttcca ttattgcggc gagaagaaac ctcttcttcg ctatttcgcg tgttcagagc
    46861 agcctgcgac atattagtcc gccagttcca gaattcgtac taccaactgc gagaagctca
    46921 tacctgcctg acgtgccgcc atcggcacca ggctgtggct ggtcataccc ggtgaggtat
    46981 tggcttccag cagataaaac tgtccatcgc tgtccagcat aacgtcaata cgtccccatc
    47041 ctttgcaacc taacgtcgtc catgctttca gcactaatgc ctgcaaattg gcctcttgtg
    47101 acgcttccag acctgcgggg cagaaatact gtgtctcatc agagagatac ttcgcctcat
    47161 aatcatagaa ggttccggac ggttgaatac gtattgacgg taaaatttct tcaccgagta
    47221 tcgcaaccgt gaactccggc ccacttagcc atttttcaat caatacttct tcatcgtgct
    47281 gaaatgccaa tcttaatgca tcttgtagag cattttctgc tactactttt gacattccca
    47341 cactggaacc ttcgcggctc ggcttaacga taaccggcaa acccagagca gaaatttctg
    47401 ctaactgctt atcgctcagg cctttttcaa actctgcgcg ggttaacgct acccacggcg
    47461 cgaccggtaa accggcacct tgccacagca gtttgctgcg tagtttatcc attgaaagcg
    47521 cagatgccat cactccgctg ccggtataag gcaagcccat cagctcaagc atgccctgca
    47581 gcgtaccatc ttcaccgccg cgaccgtgta gcgcgataaa cactttctga aaacccatcg
    47641 acttcagttg cgtcacgtcg acttctttcg ggtcgacagg atacgcatca ataccgcctt
    47701 cacgcagccc ggctaacacc gctgcgccag aattcagaga aacttcccgc tcagcggaag
    47761 tcccgcccaa caggaccgca attttatcag tcatgttgtt cttcctccgg agtttgcggc
    47821 ttcagtttga tttcagctaa agaacgggca atttttccga tattaccagc cccctgaacg
    47881 agaatcaggt cgttaccgat taataccggt gccagcatct cggctacctg cgcagggtcc
    47941 ggcaccagaa tgggatcaat tttcccacgt ccacgaattg tgcgacacag cgaacggctg
    48001 tccgctcccg gaattggcgc ttcgccagcc ggatacactt ccagcatcaa cagggtatca
    48061 acctgcgtca gcacattggc gaaatcatca tacaggtcgc gcgtacgggt aaaacggtgc
    48121 ggctgaaaca gcattactag gtttttatcc ggccagcctg cgcgcgccgc tttaatggtg
    48181 gcgtcaactt ccgtcgggtg gtggccgtag tcatcgacca gcattgccgt accgctttta
    48241 ccattcactg gctccagcgg gaattcaccg aggaaatcaa aacggcgacc agtcccctgg
    48301 aagctctcca gcgcacgcaa aatagcctcg tcgtcaattc cctcttccgt agcaaccgca
    48361 accgcagctg cggcgttcag cgcgttatga cggcctggcg cattcagggt gacgcgcatc
    48421 ggctctttgt cctggcgcag cagcgtaaag tgcccctgcg ggccaatctg ctgataatct
    48481 tctacacgca cgtcggcatc ttcgctgaag ccgtaagtcg tggtctgacg tcccacacgc
    48541 ggtaacaatt cgcggatcac cggatcatca acacacatca ccgcacgacc gtaaaacggc
    48601 aggttgtgca gaaaattaat aaaagtctgt tttaaattct caaagtcgcc ctggtaggta
    48661 tccatgtggt cggcttcgat attggtgaca atcgccacca tcggttgcag atgcaggaac
    48721 gatgcatcac tctcatctgc ttcggcaatc aggtaccgac catgccccaa acgcgcatga
    48781 acccccgcag cttttaccag cccgccgtta acgaaggttg ggtcgagccc cgcttctgcg
    48841 tagatgctgg aaaccatcgc ggtggttgtc gttttgccgt gcgttcctgc aatggcgatg
    48901 ccatgacgaa aacgcattaa ctcagccagc atttcggcac gacggatcac cggaatacgc
    48961 gcttcatgag ctgcgacaat ttccgggtta tcggcagaaa tggcgctgga aacaacgacc
    49021 acgctggcat cacgtacgtt ttccgggcga tggttgaaat aaatcgtcgc tcccagattc
    49081 attaactgct gcgtgaccgg atttggcgct aaatcggaac cactgatctg ataaccttca
    49141 ttggccagaa cttcggcaat accgcccata ccggcaccac cgatgccgac aaaatgtatg
    49201 tgccgaacgc gacgcatttc gggcacgatg gaacgcagtt ttgccaattg ttgtgtattc
    49261 attctttacg ccattaactt cttaaattca tacgatgcaa aaggcatcgc tacaattacg
    49321 cccgggcagc ccggctcact tcatttgcca ctcgctcggt ggcatccgga atggatgcag
    49381 cgcgggcgcg ttctgccatg gttaataagg tttctcgcga ccacccggcc agggtgttgg
    49441 cgacagcatc cacgctaagc tgtggctgct cgataatttt ggctgcgccc gctttttcca
    49501 gcggtagcgc attccagtat tgctggcggt ctttatgttg aaacggcaca aacaacgccg
    49561 gtagtcctgc cgcggcgatt tcactcaccg ttaaagcacc ggagcggcaa acgacgacat
    49621 ccgcccacgc atacgccgcc gccatatcat caataaattc cgtcacttta tgctgcggtt
    49681 gccccgcttc ggcatacgcc tgttcaacgg attgttgcga acctttaccg ctctgatgcc
    49741 agatagtgac tgaatcaccc agtttcgcag caacctgcgg cattgtctgg ttaagaatgc
    49801 gtgcgccctg ggaaccaccc actaccagca cacgaaccgg accttcacgt ccagccaaac
    49861 gttgctgcgg caacggcagc gccagcacat cggtacgcac cgggttaccc accacttccg
    49921 cattagggaa agcgcctgga aacgcctgca tcactttggt ggcaatcttc gccagccatt
    49981 tattggttaa gcccgcaata ccgttttgtt catgaagtac aaccggaatg cctaacgacc
    50041 acgcggccag accacctgga cctgatacat agccgcccat accgagtacc acgtcaggtt
    50101 tgtacgcttt cataatcgcc cgcgcctgac gccaggcgtt gaagatacgc agcggcgcag
    50161 ctatcagtgc ttttatacct tttccacgca gaccagagat acgaatgaaa tcaatttcga
    50221 tgccatgttt tggcactaag tccgcttcca tacggtcggc agtccccagc cagcgaactt
    50281 gccaaccctg agccattaga tggtgcgcaa ccgccagtcc cgggaataca tgtccaccgg
    50341 ttccgcctgc catcaccatt aatcgctttc cttgaccact catcgtgaac ctcgtacaaa
    50401 cgcctgcgct ttctccagac gcgtttcata atcaatacgc aacagcatca tgatggctgt
    50461 cgacataatc agtaagctcg aaccaccgta actgatcagc ggcaatgtca gacctttggt
    50521 cggtaacatc cccgccgccg cgcctacgtt aaccagcgcc tggaagctaa accagatgcc
    50581 aatagaacag gcgagaaaac cggaaaaacg gtggtcaatt tctaacgctt tgcggccaat
    50641 cgacatcgcg cgaaaagcga cgaagaatac cattaaaagt gccagcacca caccgacata
    50701 ccccagttct tcgccgataa tggcgaaaat aaagtcagtg tgcgcttccg gcagatactc
    50761 cagtttttgt accgagttac ctaaaccttg tccccaaagt tcgccgcgac caaacgccat
    50821 cagcgattgc gttaactgat agccgctgcc aaagggatct tcccacgggt tccagaatgc
    50881 ggtaacacgg cggatacggt acggttcggc gagtatcagc aacacaaccg ctgaaatgcc
    50941 cataccgata atggcaatga actgccacaa tttcgctccc gccaggaaca acatcgccag
    51001 cgtagtcaca aacaacacca ccaccgtacc aaggtctggc tgtgccagca gtaacactgc
    51061 caacaccaga atcacgccca tcggtttcag gaagccgcgc aggttattac gtacttcgtc
    51121 gcctttacgc accagatagt tggcgatata acaaaacagc gacagttttg tcagctccgc
    51181 aggctggata cgcagcaaac cgagatcgat ccaacgcgat gcccctttaa ccgagctacc
    51241 cactaccagc acgatcatca gcaggatgat agatccgagc agcatcgtgg cactgtagcg
    51301 ttgccagaac tccatcggca gacgcagcgt aatgatcgcc agaatgaatg ccaggatcag
    51361 atagacgcca tcacgcttcg cgaagaagaa cggatcgttg gttaagcgtt gccctatggg
    51421 cattgacgcc gaggtcacca tgataaagcc aatcgccgca aggccgaagg tcagccacag
    51481 taaggtgcga tcgtacatga tcaggctgtc ggtatctttt tcccgcgagc ccatcaccca
    51541 gccttttagc gccgtggaga tccagaccag gatactgaat cctggcaggc gcggcatttt
    51601 caggcgaggg agagataaac gcatcaacct aactccttcg ccagacgggc aaactcattg
    51661 cctcgttgtt caaagttctt gaactgatca aggctggcac aggccgggga gagcagaacc
    51721 atatcgccca gctgaacacg cggagccagc aagcgcatcg cttgttccat ggtttcggtt
    51781 tgttctgcca cttccgggcg tagcgccgcc agctgcgcac cgtcacgacc aaaacagtac
    51841 aaacgcacgt tgtcgccatt cagatagcgc gccagtggac tgaagtccgc cgatttacca
    51901 tcgccgccca gcaacaaatg cagcgtgccg tctacctgca agccattcaa cgccgcttcc
    51961 gtactgccga cgttagtggc tttcgaatcg ttaatccagc gtacgccatt gtgctccagc
    52021 accacttcaa agcgatgcgg cagaccggtg aaggtggtta acgctttcag gctgctggcg
    52081 cgcggcaaac cagctgcgtc tgccagtgcc agtgctgcca gcgcattggt gtagttatgc
    52141 tgcccggaaa gtttcatctc tttcacattc agcactttct cgcctttaac ccgcagccag
    52201 gtttcgccct gctgatgatt caggtgatag tcacccatgt tgacgccaaa gctgacgcag
    52261 cgttcatcag caccgcgaat cggcattgtt aaggcatcat cagcattgac gacacaaact
    52321 ttcgcatttt cgtaaatacg caattttgcc gcacggtatt gctgcaaacc aaacggatag
    52381 cggtccatat gatcttcagt cacgttcaga atggtcgccg ccaccgcctg taagctggaa
    52441 gtggtttcca gctggaagct cgacagttcc agcacgtaca gttcgcactc agcatcaagc
    52501 agcatcaacg ctggcaggcc aatattgcca cccacaccaa cgttaacccc cgccgctttc
    52561 gccatttcac ccactagcgt ggtgaccgtg cttttgccgt tagaaccggt aatcgccaca
    52621 atcggtgctt gtgcttcgcg acagaacagc tcgatatcgc caacgatttc gattccggca
    52681 tcagcggcag cgcttaagga tggatgcgcc tgtgcaatac cgggactggc gacaatcaga
    52741 tctgccgcca tcagccattc atcattcaga ctgcccgtgt ggcgttctac ggcttcgggt
    52801 aatttatcca ggccaggcgg tgtcatacgc gtatccataa cgcgcggcgt cacaccgcga
    52861 gcgaggaaaa agtccacgca ggaaagcccg gtgaggccca ggccgataat gacgacattt
    52921 ttaccctgat aatcagccat gattaacgta ccttcagcgt tgccagacca atcagaacca
    52981 gcatcagcga aataatccag aaacgcacaa tgacgcgcgg ttccggccag cctttcagtt
    53041 catagtggtg atgaatcggt gccatgcgga aaatacgttg tccgcgcagt ttaaaggagc
    53101 cgacctgcag gatgacagaa agcgtttcta ccacgaacac gccccccata atcaccagca
    53161 ggaattcctg acgtagcagt acggcgataa tgcctaacgc accacctaac gccagcgaac
    53221 ctacatcgcc cataaagacc tgcgccggat aggtgttaaa ccacaggaag cccagtcctg
    53281 ccccgactat cgccgtgcag acaatgacca gttcccctgc gtgtcgcaga tacggtatat
    53341 gcaagtagct ggcaaagttc atattgccgg tcgcccacgc caccagcgca aaaccaccgg
    53401 cgacaaatac ggtcggcata attgccaggc cgtcgagacc atcggtcagg tttaccgcgt
    53461 tgccagtacc cacaatgacg aagtaagcca gcagaatgta gaacagcccc agctgcggca
    53521 tcacatcttt aaagaatggg accaccagct gcgttgcggg cgtgtctttg ccggcaaggt
    53581 acagggcgaa ggcgacaccc agcgcaatga ccgacatcca gaaatacttc caacgagcga
    53641 tcaacccttt ggtgtcttta cgcaccactt tgcgataatc atcaacaaag ccaataacac
    53701 cgtaacctac cagcaccacc aacacgcacc agacgtacgg attggacggg taagcccaca
    53761 gcagtacgga gatcacaatc gccgtcagga tcataatccc gcccatggtc ggcgtaccgc
    53821 gcttgctgaa gtgtgattca ggaccgtcgt tacgcaccac ctgaccaaag gaaagttttt
    53881 gcaaatgagc tatcatacgc gggcccatcc acaatgagat gaacagcgcg gtcagcaggc
    53941 tgacgatggc gcgaaacgtc agataggaaa agacgttaaa gccggaataa tatttgacca
    54001 aatgttcggc cagccaaact aacatgtccc attctcctgt aaagcgcgta ctacctcttc
    54061 catggcggca ctacgtgaac ccttaactaa aatcgtaatt acctgttgct cagcaatcag
    54121 tgatttaaga cgcgtaatta acgcagtttt atcagcaaaa tgttcgccaa cgccgctggc
    54181 ggtgctgata gcatggcttt gtttacccac gcttaacacg cggtcaatac cagcagcttt
    54241 tgccgcctcg cccacctgta catggcaggc ttcgctttca gcgcccagtt ccgccatatc
    54301 gcccaccacc agcacgcggt agcccggcat ttcagccagt acctgaacag cggcggtcat
    54361 tgaaccgaca ttggcgttgt aggagtcgtc gagcagcaac tggttttctg ccagttggat
    54421 ggggaacaga cggcctggaa cagctttcag atttgccagc cccgctttga tagcatcaag
    54481 cgttgcgccc acggacatgg agagcgcagc ggctgccagc gcattcgcaa tattgtgacg
    54541 ccccggcaac ggcagcagaa catcgacgct accggttggg gtttgtaggg taaattccgt
    54601 accgtgcgag gtcacatgga tattggtggc ggtgaaatcg ctgttggcgg cattgggtga
    54661 gaaacgccac actttgcgtg agccaattac gctctgccag ttcagccagt cgttgttgtc
    54721 ggcgttcata atggcgatac cgttttccgg caggccgcta aagatttcac ctttcgcttt
    54781 cgcgacaccc gcaagcgagc caaaaccttc cagatgcgcc gctgccaggt tgttgaccag
    54841 cgcagcttcc gggcgagtca gactcacagt ccaggctatt tcgccctgat ggttcgcgcc
    54901 aagttcaata actgcgtaat cgtattccgg cgtcaagcgc aacaacgtca tcggtacacc
    54961 gatgtcgttg ttgagattgc ctgccgtata aagcgtgttg ccgcactggc ttaaaatcgc
    55021 cgccgtcatc tctttaacgg aggttttgcc ggaggaacct gtcagagcaa ccacgcgcgc
    55081 cggaacttgc tggcgaaccc atgcagccag ttcaccaaac gccagacgcg tatccttgac
    55141 gattaactgc ggcaggtcga tatccagcgg acggctgacc agtagtgcgc ccgcgccgcc
    55201 agctttcgcc tggtcggcaa aatcatgagc atcgaaacgt tcgcctttca gggcaacaaa
    55261 caggcagccc ggcgtcagtt ttcgggtatc agtggttaca gcatcaaggg tgatatctgc
    55321 accttgcagt tcaccgttga gaatgtcggt aagttggcta agggttacgc taatcatgca
    55381 atcaccccca gcagacgcgc caccgtgacg cgatcggagt agtccagacg ctgattgcca
    55441 acaatctggt aatcttcatg gcctttgccc gcgaccagta ccacatcatt ctctttagcc
    55501 tgcataacgg cgcaagtcac cgcttcagca cggccttcca tcactttggc atgtccggca
    55561 tctaacattc ccgccagaat atcgttgatg atggcacgcg gttcttcggt acgcgggtta
    55621 tcgtccgtca ccaccgccac gtcagcaaac tcttcggcaa ttgcgcccat cagtggacgc
    55681 ttacctttat cgcgatcgcc accacagcca aagacgcacc acagcttgcc cgcacagtgc
    55741 aggcgcgccg cctgtaaggc tttttccagt gcatccggcg tatgcgcgta atccaccacc
    55801 accgtcggtt tgcctggcgc agtgaacact tccatacgtc cgcaaaccgg ttgcagacgc
    55861 gcggcggttt tcagcagatc agccagtgga tagccgagtg ccagcagtgt cgccagcgcg
    55921 agcagcaggt tgctgacgtt aaaagcgccc atcagatggc tttcaatttc gccatcgccc
    55981 caacttgagc taaagcgaat cgtcgcaccg ctgtcgtgat agttcacgtc gatcgctttt
    56041 aaccagcgtc cgtgacagtt cggattaata tgatcttcca ttgataccgc aaccgcgtcc
    56101 ggcagttttg ccagccagcg gcggcccact tcatcgtcgg cgttaataat cgcctgaccg
    56161 caatgatgtt cagaataaag cagccatttc gcagcttcgt agtgttccat atcaccatga
    56221 taatcaaggt gatcgcggct taagttggta aagaccgacg ccgcaaattt caatgccgcc
    56281 acacggtgct gtaccagccc gtgggaggaa acttccattg cgcaaaacgt cgcgccctgc
    56341 tccaccagcc ccgccagctc atgctgaaca tcgactgcgg aaccggtagt attttctgtc
    56401 gggatcactt tcccaagcag accgttacca acggtgccca ttaccgcgct ggtttcgcca
    56461 agcagttggc tccactgcgc cagcagctgg gtagtcgtgg ttttgccgtt ggtgcccgtt
    56521 acgcctacga gacgtaaatt gtcagagggt tcatggtaaa agcggcccgc cagtgcagat
    56581 aaacgctcat tgagctggct gagatagatg accggtacgc cgtgcatttc acggatttca
    56641 ccgtcggtcg cctcatcttt cgctttcgcc tctgcaataa tggcagccac accttgcgct
    56701 atcgcctgcg ggatatatcg acgcccgtcc gcctgatgac ctactacagc tacaaagaga
    56761 tcgcccgccg cagccacacg gctgtcgagt gtcatctctc gcagtgctcg cgaaggtgcg
    56821 tctggcaccc acggagcaag aaggtcgcgc aaattacgat ctgccacctg tcccctcgcc
    56881 ttgattaatc acaaattcat ttttatcgcc cgttgtcagc gcatccggct cgatgttcat
    56941 ggtacgcaat acgccgccca tgatggcacc aaagaccggc gcggaaacgg cgccgccgta
    57001 gtatttaccc gcctgcggat cgttgataac aacaaccagc gcgaagcgcg gctgactcgc
    57061 aggcgcaacg cctgcggtat aagcaatata tttattgatg tagcgaccgt ccggcccgac
    57121 ctttttcgcg gtaccggttt taatggcgat acgatagcct ttaatcgccg ccttcacgcc
    57181 gccgccgcct ggtagcgcca cgctttccat catatgcacc acagtgcgga caatggatcc
    57241 cgggaagaca cgttcaccgg gaaccggggg gtcaactttg gtaatcgaca gtgggcgata
    57301 aatgccgtag ctgccgatag ttgcgtagac tcgcgctaac tgtaacggtg ttaccattag
    57361 cccgtagccg aaagagaagg tggccctctc tatgtcagac caccgttgtt tttgaggata
    57421 taagccactg cgttctccga ccaaccccaa attggtcgct tttcccagtc caaaacgtga
    57481 gtaagtatct actaacgctg aggacggcat cgctaacgcc agcttggaaa caccgacgtt
    57541 actcgacttc tgtaataccc cggtcagggt taattcgctg tagcgtgcca cgtctttgat
    57601 ttcgtggccg ttaattcgat aaggaatggt attgagtacc gagttttccc gcaccacgcc
    57661 acgttgcaac gcggtcatta ccaccatcgg tttaaccgtt gagcccggtt caaacacgtc
    57721 ggtgatggta cggttacgca tcgcctcttt cggcgtgccg ctcagattgt tagggttgta
    57781 tgacgggctg ttagccatcg ccagcacttc accggtgttg acatccacca gcacggcgct
    57841 accagattca gccttgttaa aggccaccgc gttgttcagt tcgcgataaa ccagcgcctg
    57901 caggcgttca tcaatactca gcgccaggtt gtgcgctgcc tggctgtcag tagaagaaat
    57961 atcttcaatt acgcgaccat agcggtcttt acgcacaatg cgctcacccg gctgcccggt
    58021 aagccattta tcgaaactct tctcaacgcc ctcaatccct tgactatcga cgttagtaaa
    58081 gccgatgagg tgagcagtca cttcgccgga cggatagtaa cggcgagact cttcacgcag
    58141 atgaatcccc ggcagtttca gttttttgat gtagtccgcc atgtcagggt tcacctgacg
    58201 cgccagataa ataaaacgcc ctttcgggtt ggcgttaatg cgggctgaaa gctgatccag
    58261 cggaatattg agcgcgttag ccagcgcctt ccagcggtca ccgacgctga taccgccagc
    58321 gtcatgcact tctttcgggt cagcccaaat cgcttttacc ggcacgctca ccgctaacgg
    58381 gcgaccagaa cggtcagtaa tcatgccgcg ggaggtggaa acttgctgaa cgcgaagaga
    58441 acgcatgtcg ccctctttca ccagcatatc cggggagata acttgtaacc acgctacgcg
    58501 tccgagcaga aaagccagcg ccaggagaat acagccgcat aacaacgcaa aacgccaact
    58561 gataaagttg gcatgttctt cctgacgttt tggtttctgc gttttcgccg ctgctttcat
    58621 gcgtcgcgtt tatccttatt tttgcactac gatattttct tgtgacggat caacatgctg
    58681 catttgcagc ttttccgtgg cgatcctttc cacccggcta tggtcgccga gcgcattctc
    58741 ttcaaggatc aggttgcgcc attcaatgtc taaagcatct cgctccagca ccagttgttc
    58801 gcgctgagcg gtcagtaaac gggtatggtg cgccgtggtt accacagtca ccgccgtcaa
    58861 aataatgcaa atgaacaggc agagtggcag cttcccaaat cgcaaaagat cgtcaccgat
    58921 aacaccaggc aatgcatggc gctcgtggct tcccatcgat cctttaactt tgcttagagc
    58981 ttctgtcact ctgctgatca tgcattcgtc ctctctgcaa tacgcagaac tgaactacgg
    59041 gcacgagggt tctcagccac ctcttcttcg cccggcatta acttgcctag tgctcgcagc
    59101 tgacggccac ccagtttttt gagctgctct tcagtcatcg gtaaccctgc cggaacttgc
    59161 ggaccgcggc tgttttcacg cataaaacgt ttcacaatac ggtcttccag cgagtggaag
    59221 ctgatgatcg aaagccgccc acccggggcc agcacgttga gcgagctttt tagcgcctgc
    59281 tctatctcct ccagttcact gtttacccaa atgcgcaccg cctggaaggt acgggtcgcg
    59341 ggatgtttaa acttatcttt caccggcgtt gcaacagcca cgacttccgc cagttctttg
    59401 gtgcgggtca tcggctgttc gcggttacgc tcgacaatgg cgcgggcaat gcgtttggca
    59461 aaacgctctt caccataggt tttcaatacc caagcgatat cggcttcttc tgcggtttgt
    59521 agccattcag cggctgactg cccacgggtt gggtccatac gcatgtccag cggaccatcg
    59581 cgcataaagg aaaagccgcg ctcagcatca tcaagttgcg gtgaagagac gccaagatcg
    59641 aggagaatgc cgtcgatctt gccgataaga tcgcgctcgg caacgtattc gcccagcgcg
    59701 gagaaaggtc cgtggatgat ggagaagcgc ggatcatcaa tagtcttcgc aacggcgata
    59761 gcctgcgggt cgcgatcgat cgccagcaaa cgcccctctt cgccaagctg cgagaggatc
    59821 agacgtgagt gaccaccgcg accaaaagtc ccatcaatgt agatgccatc aggacggata
    59881 ttgaggccat taacggcttc atccagcagc accgtagtat gtttatagtt ttccatcatt
    59941 ttatagagac aagtcctgca gtcgctccga taagtctccg gtagccaact gctctgcgtc
    60001 gatatcttcc ttgacctgtt gatgccaggt tgtttcatcc cacagctcaa acttgttgaa
    60061 ctgtccaacc agcatcactt cttttgtcag cccggcatgt tgccgcagta ctggcgcgat
    60121 taacaatcga cctgcgccat ccatctgaca ttcgctggca tgacctaaca gtaggcgctg
    60181 cacacggcgc tcaaccgggt tcatgctcga cagacgcgat aatttttgct cgataatttc
    60241 ccattcaggc agggggtaaa gcagcaggca cgggtgatga atgtcaatgg tgcaaaccat
    60301 ttgaccgaca gcgttctcaa gcagctgttc ccggtaacgg gtaggcactg ataagcgccc
    60361 tttgctgtcg agattgacta acgttgctcc ccggaacatg ccagcctcac cccttattcc
    60421 cactttgccc cacaatttcc cactgaaagg agtttacgga gcggaggaaa agcttgtcaa
    60481 gccagtcaca aggcataccg aggtaaaaaa cccgatgttt acggctaata tcagcgttgt
    60541 ttattttacg tccaacgtgc gcagcaaatt aacgttatga atagctgtaa gaaaaaatcc
    60601 ctcatcgtca tcaccgtttt aaaatgactc actattttcc acagcaaata taaagtgtca
    60661 gtttgcgacg cgagcggcat tttaggacat atcttccccg gttaacagtc cctgttgcgt
    60721 ctgtgtggcg cttgatgcca gcgggtttgc gcaaaagcaa agaaaagtgc ttgttaaatc
    60781 ggcaattcat ctctgcatgt aacaaaataa tgcaaaaacg cgctgtcgat atcacttaat
    60841 tattgtgaat ttcagctggt gagttaatta acgggcgggt ttaaaagaac ttatcaataa
    60901 tagtcaggaa tagtcttatt tactttaagg atattgatgt ccattcccgt actctacg
//
LOCUS       DADMKF010000006        58962 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.006, whole genome shotgun sequence.
ACCESSION   DADMKF010000006 DADMKF010000000
VERSION     DADMKF010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 58962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58962
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.006"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(216..1163)
                     /gene="dusC"
                     /locus_tag="JNC24_000340"
     CDS             complement(216..1163)
                     /gene="dusC"
                     /locus_tag="JNC24_000340"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HAY9157190.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCIAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIEKL"
     gene            1402..1800
                     /locus_tag="JNC24_000341"
     CDS             1402..1800
                     /locus_tag="JNC24_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HAY9157191.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            1797..2492
                     /locus_tag="JNC24_000342"
     CDS             1797..2492
                     /locus_tag="JNC24_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HAY9157192.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            2622..3506
                     /gene="cdd"
                     /locus_tag="JNC24_000343"
     CDS             2622..3506
                     /gene="cdd"
                     /locus_tag="JNC24_000343"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HAY9157193.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            3656..4375
                     /gene="sanA"
                     /locus_tag="JNC24_000344"
     CDS             3656..4375
                     /gene="sanA"
                     /locus_tag="JNC24_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HAY9157194.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFRREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            4378..4617
                     /locus_tag="JNC24_000345"
     CDS             4378..4617
                     /locus_tag="JNC24_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HAY9157195.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            4811..6049
                     /locus_tag="JNC24_000346"
                     /pseudo
     CDS             4811..6049
                     /locus_tag="JNC24_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416651.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
     gene            6043..7278
                     /gene="preA"
                     /locus_tag="JNC24_000347"
     CDS             6043..7278
                     /gene="preA"
                     /locus_tag="JNC24_000347"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HAY9157196.1"
                     /translation="MLTKDLSITFCGVKFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEENLAALRRLKEDYPDK
                     VLIASIMGENEQQWEELARLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPELVEKYC
                     RAVKRGSTLPMLAKMTPNIGDMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMP
                     IVNGKSSISGYSGKAVKPIALRFIQQMRTHPELRDFPISGIGGIETWEDAAEFLLLGA
                     ATLQVTTGIMQYGYRIVEDMASGLSHYLADQGFDSLQEMVGLANNNIVPAEDLDRSYI
                     VYPRINLDKCVGCGRCYISCYDGGHQAMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGC
                     IELGEVKFKKGEKEHPVTL"
     gene            complement(7405..8415)
                     /gene="mglC"
                     /locus_tag="JNC24_000348"
     CDS             complement(7405..8415)
                     /gene="mglC"
                     /locus_tag="JNC24_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HAY9157197.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(8431..9951)
                     /gene="mglA"
                     /locus_tag="JNC24_000349"
     CDS             complement(8431..9951)
                     /gene="mglA"
                     /locus_tag="JNC24_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HAY9157198.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            complement(10012..11010)
                     /gene="mglB"
                     /locus_tag="JNC24_000350"
     CDS             complement(10012..11010)
                     /gene="mglB"
                     /locus_tag="JNC24_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HAY9157199.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            complement(11290..12288)
                     /gene="galS"
                     /locus_tag="JNC24_000351"
     CDS             complement(11290..12288)
                     /gene="galS"
                     /locus_tag="JNC24_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000246891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HAY9157200.1"
                     /translation="MATVSRVLNNSTLVSADTREAVMKAVSELDYRPNANAQALATQV
                     SDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAEKERHAIEVLIRQRC
                     NALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDNLSGARMATRMLLNN
                     GHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGTPDMPGGEAAMVELL
                     GRNLQLTAIFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIPIARYTDPQLTTVRY
                     PIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQNAAAITNSTNQAM"
     gene            complement(12472..13629)
                     /locus_tag="JNC24_000352"
     CDS             complement(12472..13629)
                     /locus_tag="JNC24_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 family protein"
                     /protein_id="HAY9157201.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYGAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWRLVRDAPSVKSLFNTGVMLYLVGLGVLLLLGLISDSQTSRT
                     WTPDASAILYEKYWKLHGGVEAISNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSLRHYRRTGFVLVAIGVTINLPAIALQWQLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTMLFYHLGLFMHFDR
                     LELLAFVIPVWLANILFSVIWLRYFRQGPVEWLWRQLTLRAAGTAISKTSR"
     gene            complement(13646..14314)
                     /gene="folE"
                     /locus_tag="JNC24_000353"
     CDS             complement(13646..14314)
                     /gene="folE"
                     /locus_tag="JNC24_000353"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HAY9157202.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            14572..15408
                     /locus_tag="JNC24_000354"
     CDS             14572..15408
                     /locus_tag="JNC24_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase YeiG"
                     /protein_id="HAY9157203.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTTKAGAQRVAAELGIVLVMPDTSPRGEKVANDDGYDLGQGAGF
                     YLNATQPPWATHYRMYDYLRDELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWGIKAFSTYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            complement(15440..17431)
                     /gene="cirA"
                     /locus_tag="JNC24_000355"
     CDS             complement(15440..17431)
                     /gene="cirA"
                     /locus_tag="JNC24_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
                     /protein_id="HAY9157204.1"
                     /translation="MFRLNPVVRVGLCLSAISCVWPVLAVDDDGETMVVTASSVEQNL
                     KDAPASISVITQEDLQRKPVQNLKDVLKEVPGVQLTNEGDNRKGVSIRGLDSSYTLIL
                     VDGKRVNSRNAVFRHNDFDLNWIPVDSIERIEVVRGPMSSLYGSDALGGVVNIITKKI
                     GQKWSGTVTVDTTIQEHRDRGDTYNGQFFTSGPLIDGVLGMKAYGSLAKREKDDPQNS
                     TTTDTGETPRIEGFSSRDGNVEFAWTPNQNHDFTAGYGFDRQDRDSDSLDKNRLERQN
                     YSVSHNGRWDYGTSELKYYGEKVENKNPGNSSPITSESNTVDGKYTLPLTAINQFLTV
                     GGEWRHDKLSDAVNLTGGTSSKTSASQYALFVEDEWRIFEPLALTTGVRMDDHETYGE
                     HWSPRAYLVYNATDTVTVKGGWATAFKAPSLLQLSPDWTSNSCRGACKIVGSPDLKPE
                     TSESWELGLYYMGEEGWLEGVESSVTVFRNDVKDRISISRTSDVNAAPGYQNFVGFET
                     GANGRRIPVFSYYNVNKARIQGVETELKIPFNDEWKLSINYTYNDGRDVSNGENKPLS
                     DLPFHTANGTLDWKPLALEDWSFYVSGHYTGQKRADSATAKTPGGYTIWNTGAAWQVT
                     KDVKLRAGVLNLGDKDLSRDDYSYNEDGRRYFMAVDYRF"
     gene            complement(17723..19192)
                     /gene="lysP"
                     /locus_tag="JNC24_000356"
     CDS             complement(17723..19192)
                     /gene="lysP"
                     /locus_tag="JNC24_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HAY9157205.1"
                     /translation="MGSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            complement(19397..20278)
                     /locus_tag="JNC24_000357"
     CDS             complement(19397..20278)
                     /locus_tag="JNC24_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY9157206.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            20377..21426
                     /locus_tag="JNC24_000358"
     CDS             20377..21426
                     /locus_tag="JNC24_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HAY9157207.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATESVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            21500..22357
                     /gene="nfo"
                     /locus_tag="JNC24_000359"
     CDS             21500..22357
                     /gene="nfo"
                     /locus_tag="JNC24_000359"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HAY9157208.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAVEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKFRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQDDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            22361..23449
                     /locus_tag="JNC24_000360"
     CDS             22361..23449
                     /locus_tag="JNC24_000360"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HAY9157209.1"
                     /translation="MNDREKQILKILRRNPLIQQNEIADILQISRSRVAAHIMDLMRK
                     GLIKGKGYILTEQDYCVVVGAINMDIRGMADIRYPQAASHPGSVHCSAGGVGRNIAHN
                     LALLGRDVHLISAIGNDFYGETLLEETRRAGVNVSNCIRLHGHSTATYLAIANKQEET
                     ILAINDTHILQQLTPQLLNTSRDLIRHAGVVLADCNLTPEALEWVFTIADEIPMFVDT
                     VSEFKANKVKNWYSRIHTLKPTQNELEILWGQPIKDDNDRIRAVNSLHQQGVKRIFVY
                     LKDESVFCSDKDGEQFLLTAPAHTTVDSFGADDGFMAGLVYSFLEGYSFRDSARFAVA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(23505..24755)
                     /locus_tag="JNC24_000361"
     CDS             complement(23505..24755)
                     /locus_tag="JNC24_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HAY9157210.1"
                     /translation="MDVMRSVLGMVVLLAIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEAAATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTGGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(24855..25796)
                     /gene="rihB"
                     /locus_tag="JNC24_000362"
     CDS             complement(24855..25796)
                     /gene="rihB"
                     /locus_tag="JNC24_000362"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HAY9157211.1"
                     /translation="MEKRKIILDCDPGHDDAIAIMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRQQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERAGGPAGEMFSD
                     IMNFTLKTQFENYGLAGGPVHDATCIGYLINPDGIKTQEMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            25926..26624
                     /gene="yeiL"
                     /locus_tag="JNC24_000363"
     CDS             25926..26624
                     /gene="yeiL"
                     /locus_tag="JNC24_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
                     /protein_id="HAY9157212.1"
                     /translation="MKEIHNNDLKQQLMSESAFKDCFLTDVSADTRLFHFLARDYIVQ
                     EGQQPSWLFYLTRGRARLYATLANGRVSLIDFFAAPCFIGEIELIDKDHEPRAVQAIE
                     ECWCLALPMKHYRPLLLNDTLFLRKLCVTLSHKNYRNIVSLTQNQSFPLVNRLAAFIL
                     LSQEGDLYHEKHTQAAEYLGVSYRHLLYVLAQFIHDGLLIKSKKGYLIKNRKQLSGLA
                     LEMDPENKFSGMMQ"
     gene            complement(26691..27941)
                     /locus_tag="JNC24_000364"
     CDS             complement(26691..27941)
                     /locus_tag="JNC24_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HAY9157213.1"
                     /translation="MDIMRSVAGMVVLLAIAFVLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRMNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSIIEAAANGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFANVSLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTSGTLDVKTIAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(28035..28973)
                     /locus_tag="JNC24_000365"
     CDS             complement(28035..28973)
                     /locus_tag="JNC24_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HAY9157214.1"
                     /translation="MSELTLSPELLQISAEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEEIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSPFEVSIRLDSAREIARA
                     MAVKWQTGLNGGLVVANPIPEQFAMPEETINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLVG"
     gene            complement(28961..29902)
                     /locus_tag="JNC24_000366"
     CDS             complement(28961..29902)
                     /locus_tag="JNC24_000366"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HAY9157215.1"
                     /translation="MREKDYIVIIGSANIDVAGYSHHPLNYADSNPGKIKFTPGGVGR
                     NIAHNLALLGKNAWLLSAVGGDFYGQSLLAQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMSISDCISAEFLAQHQEFIRGAKVIVADCNLSEEVLVWVLENSGETP
                     VFIDPVSAWKCVKIRDHLSKIHTLKPNRLEAETLSGIALSGREDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDINGESGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFIDSV
                     RFAQGCSSMALACEYTNNPELSIANVRSLVENTECLN"
     gene            complement(30360..32051)
                     /gene="fruA"
                     /locus_tag="JNC24_000367"
     CDS             complement(30360..32051)
                     /gene="fruA"
                     /locus_tag="JNC24_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HAY9157216.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVAATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTAQELDKAVAEATPYEPAGKAQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILDGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPETQIVEKNA"
     gene            complement(32068..33006)
                     /gene="fruK"
                     /locus_tag="JNC24_000368"
     CDS             complement(32068..33006)
                     /gene="fruK"
                     /locus_tag="JNC24_000368"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAY9157217.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTLADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            complement(33006..34136)
                     /locus_tag="JNC24_000369"
     CDS             complement(33006..34136)
                     /locus_tag="JNC24_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HAY9157218.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDEHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEVGAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            34504..35685
                     /gene="setB"
                     /locus_tag="JNC24_000370"
     CDS             34504..35685
                     /gene="setB"
                     /locus_tag="JNC24_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HAY9157219.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLADVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPVILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLFCLLRIKD
                     V"
     gene            complement(35682..35936)
                     /locus_tag="JNC24_000371"
     CDS             complement(35682..35936)
                     /locus_tag="JNC24_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HAY9157220.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            36091..36663
                     /gene="yeiP"
                     /locus_tag="JNC24_000372"
     CDS             36091..36663
                     /gene="yeiP"
                     /locus_tag="JNC24_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HAY9157221.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLVKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            36886..38352
                     /locus_tag="JNC24_000373"
     CDS             36886..38352
                     /locus_tag="JNC24_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAY9157222.1"
                     /translation="MKTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERGLTPFTVLSCDNIPDNGHVVKNAVLGMAEKCSPELAGWIKKHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFIAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFVHISDCMQDRVFRHAARTLMLDE
                     QAPTLQIKDVDLTQYADKLIARFANPSLKHKTWQIAMDGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNTLKI"
     gene            38470..39456
                     /locus_tag="JNC24_000374"
     CDS             38470..39456
                     /locus_tag="JNC24_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAY9157223.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYELWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            39495..40208
                     /gene="lpxT"
                     /locus_tag="JNC24_000375"
     CDS             39495..40208
                     /gene="lpxT"
                     /locus_tag="JNC24_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HAY9157224.1"
                     /translation="MIKNLPQIVLLNIVGLALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIMGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            40620..41186
                     /gene="mepS"
                     /locus_tag="JNC24_000376"
     CDS             40620..41186
                     /gene="mepS"
                     /locus_tag="JNC24_000376"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HAY9157225.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            41367..42923
                     /locus_tag="JNC24_000377"
     CDS             41367..42923
                     /locus_tag="JNC24_000377"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HAY9157226.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLIIDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALNINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVAEGVETPEQARWLSERGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTPQW"
     gene            43005..44819
                     /locus_tag="JNC24_000378"
     CDS             43005..44819
                     /locus_tag="JNC24_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAY9157227.1"
                     /translation="MIVRILLLFIALFTFGVQAQAIKESYAFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFMTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPSKEDMLSLFSLPIFPEKYWKDHKLSDPLATPPLASGPYRVTSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRPVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNETGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRSRDYDMMPRLWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            44820..45914
                     /locus_tag="JNC24_000379"
     CDS             44820..45914
                     /locus_tag="JNC24_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HAY9157228.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HVFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            45914..46939
                     /locus_tag="JNC24_000380"
     CDS             45914..46939
                     /locus_tag="JNC24_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY9157229.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FEPNKAV"
     gene            46941..48530
                     /gene="yejF"
                     /locus_tag="JNC24_000381"
     CDS             46941..48530
                     /gene="yejF"
                     /locus_tag="JNC24_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HAY9157230.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LITSQGSIVFDGQPLQDLNRRQLLPTRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFAAPQQEYTRQLLALS"
     gene            complement(48534..48878)
                     /locus_tag="JNC24_000382"
     CDS             complement(48534..48878)
                     /locus_tag="JNC24_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9157231.1"
                     /translation="MTSLQLSIVHRLPQNFRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(49211..50331)
                     /locus_tag="JNC24_000383"
                     /pseudo
     CDS             complement(49211..50331)
                     /locus_tag="JNC24_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311101.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
     gene            complement(50359..51054)
                     /gene="rsuA"
                     /locus_tag="JNC24_000384"
     CDS             complement(50359..51054)
                     /gene="rsuA"
                     /locus_tag="JNC24_000384"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HAY9157232.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLVSPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            51204..52964
                     /locus_tag="JNC24_000385"
     CDS             51204..52964
                     /locus_tag="JNC24_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAY9157233.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NIDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRSDEKALFRDCIYELPLRYMIKHSYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDTPGAERDVLIENFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRPPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            53089..53373
                     /gene="rplY"
                     /locus_tag="JNC24_000386"
     CDS             53089..53373
                     /gene="rplY"
                     /locus_tag="JNC24_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HAY9157234.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(53512..54519)
                     /gene="yejK"
                     /locus_tag="JNC24_000387"
     CDS             complement(53512..54519)
                     /gene="yejK"
                     /locus_tag="JNC24_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HAY9157235.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNTKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWEPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            54701..54928
                     /locus_tag="JNC24_000388"
     CDS             54701..54928
                     /locus_tag="JNC24_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HAY9157236.1"
                     /translation="MPQISRYSDEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMATNL
                     INTSIAPAQRQAIANSFARALQSSINEDKAH"
     gene            54948..56708
                     /gene="pbgA"
                     /locus_tag="JNC24_000389"
     CDS             54948..56708
                     /gene="pbgA"
                     /locus_tag="JNC24_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin transport protein PbgA"
                     /protein_id="HAY9157237.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDEQTATQWINWLGHYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQINRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEDEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            56783..56859
                     /locus_tag="JNC24_000390"
     tRNA            56783..56859
                     /locus_tag="JNC24_000390"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:56817..56819,aa:Pro,seq:ggg)
     gene            complement(56962..58962)
                     /gene="yejO"
                     /locus_tag="JNC24_000391"
                     /pseudo
     CDS             complement(56962..58962)
                     /gene="yejO"
                     /locus_tag="JNC24_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter YejO"
BASE COUNT        14513 a        15390 c        14398 g        14661 t
ORIGIN      
        1 gaacaaaatc gccgctgcgc ggtgccctcg gcttatccct tacggctacc gggtcgggcg
       61 cgaggtaaca tccctgtaaa acgcgccctc agcccacctc catgtgggct gccccggcct
      121 tcagggaacg cctcggcaat tttgacgcca ccaaacaacc gtgcggccta ttgataaaga
      181 gctaacacat tgtcaaaaaa catcactatg gttttttaga gtttctcgat atcaattgcc
      241 tgaatagccc ttgcaatatc aggggaatta ttcaacaccc gaacatgctg aaataattcc
      301 gttgcttcat cgtattcttt acgcaaataa ctcaaccact gtttaatccg cgcaacgtga
      361 tataacccgg tatcgccctg cttttccaga cgggtatatt tttgcagcaa agcaaccacc
      421 tccggccacg gcattcgcgg ttcgttatat tttactaccc ggctcaggtt gggaatattg
      481 agcgccccgc gaccaatcat cactgcgtcg cagccgctga tcgcaatgca ttgttgcgcg
      541 ctctgccagt cccagatttc gccgttggca atcaccggaa tattcagccg ctggcgaatc
      601 tcgccaatcg cctgccagtc aatatgctcc gcgcggtaac cctgctcttt tgtccgtcca
      661 tgcaccacca gctccgtagc accagcctgt tgaaccgcat cggcgatttc aaatttcttc
      721 tcaccgctgt cccagcccag acgcactttc acgctgacgg gcaaatgcgc cggtacagct
      781 tcacgcatcg cttttgcacc ctggtagatg agttcaggat ctttgagtaa cgtcgccccg
      841 ccgccgctgc cgttaaccgt tttcgacggg cagccgcaat tgagatccac gccccaggaa
      901 cctaattcca ccgcacgggc ggcgttctct gccagccatt gtgggaactg acctaacaac
      961 tgaacgcgca ccagcgtacc ggatggtgtc cggctggcgt tttgtagctc agggcaaatg
     1021 cgatgaaaga cttttaccgg cagcagttga tccaccacgc ggacaaactc ggtgatgcac
     1081 agatcgtagt cgttaacttc ggtcagcaat tctcgcacca gagagtcaag cacaccctcc
     1141 atcggagcca gtaacacacg catatcttca cccggaaaaa aatgaggcgc tatgttagcg
     1201 cctatggtca acggattaaa ggtctgcaaa gagggattgc tgcaaactgt aatccctctc
     1261 tatgcttgat gtatgcctga gttcgttgcg cattgcgtaa tgccggggag cgaaaaaata
     1321 agcgctgatt tcttaatgtg atcggtagca cgttttacga attaattgta tgatgaatcc
     1381 atctcatctg gggtgttgat tatgagtaag acactgaaca ttatctggca atatttacgc
     1441 gctttcgtcc tgatttatgc ctgcctgtat gcaggcattt tcattgcttc cctgctaccg
     1501 gtaaccattc cgggcagcat catcgggatg ctgatcctgt ttgtcctgct ggccttgcaa
     1561 attcttccgg caaaatgggt caatccgggg tgctacgtac tgattcgcta tatggcgcta
     1621 ttgtttgtgc cgattggcgt aggcgtcatg caatattttg atttgctccg cgcacagttt
     1681 ggcccggtag tggtttcctg tgcagtcagt acgctggtgg tttttctggt ggtgagctgg
     1741 agttcacaac tggtacacgg tgaacgtaaa gtcgtaggtc agaaaggatc agaagaatga
     1801 tggcgaatat ctggtggtca ttaccgctga cgttgattgt cttttttgcc gcccgcaaac
     1861 tggcggcacg gtataagttt cccctgctta acccgttgct ggtagcaatg gtggtgatca
     1921 ttcctttttt aatgctgacc ggcatctctt acgacagcta ctttaaaggt agcgaagtgc
     1981 taaacgatct gctgcaaccg gcggtcgtcg cgctggccta tcctttatat gagcagctac
     2041 accagatccg cgcgcgctgg aaatcgatca tcaccatctg ttttatcggc agcgtggttg
     2101 cgatggtgac ggggacttcc gtggcattgt tgatgggcgc ttcaccggaa atcgccgcgt
     2161 caatcctgcc gaaatcagtc accacgccta ttgcaatggc ggttggcggc agtattggcg
     2221 gtattccggc aatcagcgca gtttgcgtga ttttcgtcgg catcctcggc gcggtatttg
     2281 gccatacatt gcttaatgcg atgcgtattc gtaccaaagc tgcgcgcggt ctggcaatgg
     2341 ggacagcctc gcacgccctc ggtacggcgc gctgcgccga gctggattat caggaaggtg
     2401 catttagttc gctggcgctg gtgttatgcg ggataattac ttcgctgatc gcaccgttcc
     2461 ttttcccgat tattctggca gtaatgggct aaaatttgcg atgcgtcgcg catttttgat
     2521 gtaagtttca cgcgttgcat aattaatgag attcagatca catataaagc cataacgggt
     2581 tcgtaaactg ttatcccatt acatgattat gaggcaacgc catgcatcca cgttttcaaa
     2641 ccgcttttgc ccaacttgcg gataacttgc aatctgcact ggaacctatt ctggcagaca
     2701 agtacttccc cgctttgttg accggggagc aagtctcatc gctgaagagc gcaacggggc
     2761 tggacgaaga cgcgctggca ttcgcactac ttccactggc ggcggcctgt gcgcgtacgc
     2821 cattgtcgaa ttttaatgtt ggcgcaattg cgcgcggtgt gagcggaacc tggtatttcg
     2881 gtgccaatat ggaatttatt ggtgcaacaa tgcagcaaac cgttcatgcc gaacaaagcg
     2941 cgatcagcca cgcctggttg agtggtgaaa aagcgcttgc agccatcacc gttaactata
     3001 cgccttgtgg tcactgccgt cagtttatga atgaactgaa cagcggtctg gatctgcgta
     3061 ttcatctgcc gggccgcgag gcacacgcgc tgcgtgacta tctgccggat gcctttgggc
     3121 cgaaagatct ggagattaaa acgctgctga tggacgaaca ggatcacggc tatgcgctga
     3181 cgggtgatgc gctttctcag gcagcgattg cggcggcaaa ccgttcgcac atgccttaca
     3241 gtaagtcgcc aagcggtgtc gcgctggaat gtaaagacgg tcgtattttc agcggcagct
     3301 acgctgaaaa cgccgcattc aacccgactc tgccaccgtt gcagggggcg ttaattctgt
     3361 tgaatctcaa gggttatgat tacccggata tccagcgcgc ggttctggca gaaaaagccg
     3421 atgcgccgtt gattcagtgg gatgccacct ccgcaacgct gaaagctctc ggctgtcaca
     3481 gtatcgaccg cgtgcttctc gcttaagcct ggtgccggat gcggcgtgaa cgccttgtcc
     3541 ggcttgccag ccctctcctg gtgtcgaaat tcccggcaaa cagtttgccg tttcttgcgc
     3601 aaaaccagcg ggtaaagtag cctgatggaa attttcctta gatcgagtct cctgcatgtt
     3661 aaagcgcgtg ttcctcagcc tgttagtcct gatcggcttg ctgctgttga ctgtgctcgg
     3721 cctcgatcgc tggatgagct ggaaaaccgc gccttatatc tacgacgaat tgcaggatct
     3781 cccctaccgc caggtcggcg tggtgctcgg cacagcaaaa tattatcgta ctggcgtaat
     3841 taatcagtat tatcgctacc gcattcaagg agcgattaat gcctataaca gcggtaaggt
     3901 aaattatcta ttactgagcg gcgataacgc attgcaaagt tataatgagc cgatgaccat
     3961 gcgcaaagat ttaatcgctg ctggtgtcga cccatcagat attgttctcg attacgcagg
     4021 ctttcgtacg ctggattcca tcgtgcgtac acgcaaagtg ttcgatacta atgatttcat
     4081 tattatcacc caacgtttcc actgtgagcg ggcattattt attgcgctgc atatggggat
     4141 tcaggctcag tgttatgccg taccgtcacc gaaagatatg ctgtcagtac gtattcgcga
     4201 atttgccgcc cgtttcggtg cgctggctga cctttatatt tttagacgtg aaccgcgttt
     4261 tttagggccg ctggtcccta ttccggctat gcaccaggtg ccggaagatg cgcaggggta
     4321 tcccgccgtc acacccgaac agttacttga attacaaaag aaacaaggaa agtagttatg
     4381 gatgtacagc agttttttgt cgttgccgtt tttttcctta tcccgatatt ttgtttccgc
     4441 gaagcatgga aaggctggcg cgcaggcgcg attgataaac gggttaaaaa tgcaccggaa
     4501 ccggtgtatg tctggcgagc aaaaaatccc ggactctttt tcgcttatat ggtggcatat
     4561 atcggcttcg gaattttatc tatcggcatg attgtttatc ttattttcta tcgttaatat
     4621 tttctctttt ctgttaatta ttgagaataa ttattacttc actcaataag ctgcggatat
     4681 aacattctga accgcagcta tttgtgaatc ctttcacagt ttaaatttcc ccacacgctt
     4741 agccttaata tcagcacgca atatttattt agcgttcgcc ttaattactc atagcattaa
     4801 ggaagatcac atgccgcaac aaaattatct ggatgaactc actccggctt ttacgccttt
     4861 actggcgatt aaagaagcct ctcgctgttt attatgtcat gacgctccct gtagtcaggc
     4921 ttgcccggca cagaccgatc cggggaaatt tattcgctca atctactttc gtaattttaa
     4981 aggcgctgcc gagacaattc gcgaaaataa tgccctcggt gccgtttgtg ccagagtgtg
     5041 cccgacggag aaattatgcc aaagcggttg tacccgtgcc ggtgttgatg ctcccattga
     5101 tatcggccgc ttacagcgtt ttgttactga ttttgaacaa caaaccggga tggaaattta
     5161 tcagcccggt actaaaacgc tcggcaaagt cgcgattatt ggcgcaggtc ctgccggatt
     5221 gcaggccagt gtgacactga caaaccaggg ttatgacgtc acgatctatg agaaagaagc
     5281 acaacccggt ggctgattgc gtaacggcat tccgcaattc cggttaccgc aatcagtact
     5341 ggatgcagag atcgcccgta ttgaaaaaat gggcgtgacc attaagtgca acaacgaagt
     5401 cggtaacaca ctcacccttg agcagctgaa agcagaaaac cgcgcggtac tggtcaccgt
     5461 ggggttatca agcggttccg ggctaccgct gtttgagcat agtgacgttg agattgccgt
     5521 cgacttcttg caacgtgcac gacaggcgca aggcgatatc agcattccac aaagcgcatt
     5581 aattatcggc ggcggtgatg tcgcgatgga cgtagccagc acgctgaaag ttctcggctg
     5641 tcaggcggta acttgcgtag cgcgtgaaga gttagatgag tttccggcaa gcgaaaaaga
     5701 gtttaccagc gcccgggagc tgggcgtttc gatcatcgat ggattcacgc cagtagccgt
     5761 ggaaggcaat aaagtcacgt ttaagcatgt acggttatcg ggcgaactga cgatggcggc
     5821 agataaaatt attctcgccg tcggtcagca tgccagacta gatgcctttg cggagttaga
     5881 gccgcagcgt aacaccatca aaacacaaaa ttaccagacc cgcgacccgc aagtctttgc
     5941 tgctggcgat attgttgagg gagacaaaac cgtggtctat gccgtgaaaa ccgggaaaga
     6001 agccgccgag gcgattcatc actatttaga gggagcttgc tcatgttaac gaaagatctt
     6061 tcgattactt tttgcggcgt gaagtttccc aacccgttct gcctctcttc ttcgccggta
     6121 ggcaactgct atgagatgtg tgccaaagcc tacgacacag gttggggcgg tgtggtgttt
     6181 aaaacgatcg gcttttttat cgccaacgaa gtctcgccgc gttttgatca tctggtgaaa
     6241 gaagataccg gttttatcgg cttcaaaaat atggagcaga ttgccgagca tccgctggag
     6301 gagaatctgg ccgccctgcg tcggctgaaa gaagattacc cggacaaagt attgatcgct
     6361 tcgatcatgg gggaaaatga gcagcaatgg gaggagctgg cgcgcctggt gcaagaagct
     6421 ggcgcggata tgatcgagtg taacttctcc tgtccgcaaa tgacttctca tgcgatgggt
     6481 agcgatgtcg ggcaaagccc ggagctggta gaaaaatatt gtcgggcagt gaaacggggt
     6541 tccacgctgc caatgctggc gaagatgacg ccgaatatcg gtgatatgtg cgaagtggcg
     6601 ctggcggcga agcgcggcgg cgcagatggc attgcggcga ttaacaccgt taaatccatc
     6661 accaatatcg atcttaatca gaaaatcggt atgccgatcg ttaacggaaa atcaagtatt
     6721 tccggatatt ccggtaaagc ggtaaaaccg atcgccctgc gcttcattca gcaaatgcgc
     6781 acccatccag aactgcgcga tttcccaatc agcggtatcg gcggcattga aacctgggag
     6841 gatgcggcgg agtttttatt gctcggcgca gcaacgttac aggtcaccac cggcattatg
     6901 cagtacggct atcgcatcgt agaagatatg gcgagcgggt tatcgcatta tctcgccgat
     6961 cagggatttg attcgttgca ggagatggta ggtctggcga ataacaatat tgtcccggcg
     7021 gaagatttag accgcagtta tattgtctat ccccgtatca atcttgacaa atgtgttggc
     7081 tgtggacgct gttatatttc ctgttacgac ggcggtcacc aggcgatgga atggagcgag
     7141 aaaacccgca caccgcattg taataccgag aaatgtgtgg gttgtctgct ttgtggtcac
     7201 gtctgcccgg tgggttgtat tgagctcggg gaagtgaagt ttaagaaagg cgagaaagaa
     7261 cacccggtaa cgttgtagaa cccgcgtcac atccggcatt tggttcctgt tgccggatgc
     7321 ggcgtgaacg ccttatccgg cctacaaaac cacacagatc atgatgttat gtgcgtttat
     7381 gaccgaatgc ggaccacatt cacatcattt cttacgcgcg tatttcagtg aatccagcgc
     7441 tacggcgaag ataataatcg cccctttgat gatgtactgc cagtatgggt ttacgccgat
     7501 atacgtcagg ccatagttga tgacggtaaa aataatcacc ccggtcacca cgccaatcac
     7561 cgtccccaca ccgccgctga acgatacacc gcctaccacg cacgccgcga tagcatccag
     7621 ctcatacata aagccgaggt tgttggtggc agagccgata cgtccggctt ctaacatccc
     7681 gccaaaggca tagaacacgc cagacaacgc gtagatcatc agcaggttca ggccgacgtt
     7741 gacaccagat acttttgccg cttccgggtt accgccaatg gcaaaaatgt tcttaccgaa
     7801 gcgggttttg ttccacaaca cccagacgaa cgccaccgca atcaacgcgt agaaggtgat
     7861 gtaagagaga cggaaactcc ccagcgcgac aaagccctga gcaaaggtag agaagccact
     7921 gtcaaaacca gaaattggcg acgccccgac aaagtcgtaa tagagcgagt tgatgccata
     7981 gacgatgatc atcgtgccga gcgtggtaat gaacggcgtc acgttgagat aagcgataat
     8041 cagaccgttg atcaaaccga tcaccgcacc aatggcacag acaatcagaa taaccagcgc
     8101 aatcggcatc gtcgccattt ccgggaacac tttgttggcg ttatccatgg actgcaataa
     8161 tgtcgcagcc accactgccg ccagccctac ctgacgacca gcagaaagat cggtcccctg
     8221 ggtgacaatt aaccctgcca caccgagcgc gataataata cgcaccgatg actgggtgag
     8281 aatattactt aagttcaaca gacttaaaaa tgttgggtcc tggaaaataa taatcgccag
     8341 caaaactaaa agaacgacgt aaataccgcc ctctttcagg taagtaagaa aacttttctt
     8401 atttaacgca ctcatgggaa gcccctaatc ttaaaggtgc aaagacgcaa gacgcagaat
     8461 ttcgttttgc gttgttgttt ttgtatcgac aattccggaa acgagaccat tgctcatgac
     8521 cagaatacgg tctgttatcc ctaacaactc aggcatttca gaggagataa taataatccc
     8581 cttgcctttc ttcgccagtt cggcaattaa ctgataaatt tcaaacttcg ccccgacatc
     8641 aataccgcgc gtcggttcat cgagcattaa tatttctggt tgcgttagta gccagcgacc
     8701 aataatcacc ttttgctgat taccaccgga gagcgaacca atttgcgtcc gatgacccgg
     8761 cgtttttacc cgcatcgaat caatcaccca ctgggtatcg cttttcatcc gcgagttatc
     8821 cagtaaacca actttatttt tgtagttgcg aatattggaa attaacgagt taaaaccaat
     8881 atccagatag gcataaattc ccgttgagcg gcgctcctca gttaccagtg caaatccgtg
     8941 gtttatggct tcgttggcat tatggttatt gatctgtttg ccgtgcaacg taatggtgcc
     9001 agccgatttc tcgcgaatac caaataacgt ctcaacaata tcggtacgtt tcgcccccac
     9061 cagcccggca ataccgagga tctccccttt atgcagatca aacgagacat cgcgaatcga
     9121 cggctggcgc agtgacgtta ggttacgcac ctcgaggatg acttcgcccg gcttgttttc
     9181 tttgtcagga aaacgctgat taagagaacg cccaaccatc atggcgatga tcttgtccat
     9241 cgtcagtcct gccagcggtt cggtggcgat ccactgaccg tcgcgcaata cggtaacttc
     9301 atcacataac tggaagattt cttccatttt atgcgagata taaacaatac cgcagccgcg
     9361 ctcttttaat ttacgaataa tagtgaacag atgattgacc tctttttcgg ttaacgaaga
     9421 agtcggttca tccataatca caattttcgc gttataggaa aacgctttgg cgatttcgat
     9481 catctgcatt tgcgaaacgg ataatgtgcc gacgcgcgca cgcggatcga tatcaatatc
     9541 cagttcatca aaaattgctt tggtttcgcg gtacattttg tcctgatcga caaacatgcc
     9601 tttggtggga tatcgcccca gccacatgtt atccatcacc gaacgttgta ataccaggtt
     9661 taactcctgg tgtaccatcg aaataccatt ttccagtgcc tctttggcgg aatggaaatc
     9721 gatctcttta ccctggaata aaatggtgcc ggagtctttt tgataaatac caaacaggca
     9781 ttttaataat gtcgattttc ctgcaccgtt ttcccccatt aatgcatgga tagaatgtgg
     9841 ccggactttt aaattaacgt tatcaagtgc cttaacacca ggaaaggact tgttgatacc
     9901 gctcatttcc aacaagtatt ccccggaaga cggagtcgtt gagctgacca tataatttta
     9961 ccttgttggc catacaataa gggcgcagta aacgactgcg cccaatcagt cttatttctt
    10021 gctgaattca gccaggttgt ctttatctac gccaacataa ggtacgcgga ccactttgtt
    10081 gtcgattttc cagttggtgc catcagccgc acctttgcca tcggccaggt ttttcgccag
    10141 atcaaaggtc gctttcgcct ggttgttagc atcgttcagt actgtgcccg ccagtgcacc
    10201 ggatttcacc agcgccagcg cttctggcag cgcatcgacg ccaaacaccg gaatgctgga
    10261 cttgttgtgt gctttcagtg cttctaccgc gcccattgcc atcgcatcgt tgttggcgat
    10321 aaccacttcg attttgttgg cgttcgggcc agacagccag gcgtccatct tatctttcgc
    10381 ctgagcggtg tcccacattg cggtatctaa ctgtaactgt tcagttttga tgcctttatc
    10441 gttcaattct ttaatcacgt aagtggtacg tgcttctgca tccggatggc ccggttcacc
    10501 tttcagcagt acgaactgaa tctgaccgtc tttgttcaga tcccaaccct gattcgccgc
    10561 ccagtgttta gcaatcaaat cgccctgaat aatgccggac tctttggagt cagtgccaac
    10621 gtagtaggct ttgtcgtagc tatccagcgc cttacgagac ggttctttgt tgaagaaaac
    10681 caccggcacg ttttgcccac gcgctttctc aatcaccgta cccgcagctg ccgggtcaac
    10741 caggttgatt gccagtgcct tcaccccttt cgccagcaat acgtcgatct gatcgttctg
    10801 cttggactgg tcattctgag aatcattcat cagcagctga acatctggcg cggctttcgc
    10861 atcttgctca atagccttgc gcactacaga cataaagtta tcgtcgtact tatagattgt
    10921 tacaccaatg cgagtatcag cagcgtgtgc agcggcaccg aataacatgc tggccatcac
    10981 agcagacagg gttaacacct tcttattcat ggtatctccg gtttttctta tgcagggtag
    11041 tgcttgagat aaatgctcgg cggggcagta gagttaatga agtgttactg aacgccgaag
    11101 ctcacttttt taaattcgtt cttccatgcc cggtaacgct ccagaaaacg gctttaattg
    11161 ttgttatgac gctattacct cggtgaaagt gattattcac tgttacatac gggttacaac
    11221 gttaaaacgg tgcaatcata gctatcacat tgttaagata ctgtgaaatc actcacagat
    11281 tgaaagcggt tacatcgcct gatttgttga gttagtgatc gccgccgcat tctggcgcgt
    11341 tgcgacagaa tggcgacgca ctaacgtcgg cataaaacag tggctggcac gaggatcaat
    11401 attgcctgct gccccctgca aggccagttc ggtggctaat ttagccattg aagcaatggg
    11461 ataacgcacg gtcgttaatt gcgggtcggt gtaacgggcg atgggaatat catcgaaacc
    11521 gatgattgag agatgtaacg gaatcgcaat gccattatct tttaatgctg tcagtgcgcc
    11581 agcggccata ttgtcgttat aagcaaatat agcggtaagt tgtagattgc gccccagcag
    11641 ttcaaccatc gccgcctcac cgcccggcat gtccggcgta ccagtgccaa tccagctttc
    11701 cggcggaata atatcctgct ctttcaacgc actcatccag cctgctttac gcatggcgtc
    11761 atcttcaatg ccgtgactgg aagaaagata accaatacgt tgatgaccgt tattcagcaa
    11821 catgcgcgtc gccattcggg caccgctgag attatccagg caaacgcaac gatgggcgta
    11881 ccccggcaca acgcggttga ttaacaccat accgggaatg ttatccataa attgcgccag
    11941 ttcatcgtca ctcaatgctt ttgagtgaac aatcaacgca ttacaacgct ggcgaattaa
    12001 cacctcaatg gcgtgacgct ctttttccgc ttcatgatag ctattgccga ttagcacgta
    12061 tttctgatgc tgctgagcga ctagatccac cgcttttacc agcgcgccga aaaacgcatc
    12121 agaaacgtcc atcaccacca cgccaatggt gtcgctaacc tgagttgcca gcgcctgggc
    12181 attggcgttt ggccgataat ccagctcact cacggctttc attactgctt cacgcgtgtc
    12241 ggcactgacc agcgtgctgt tattgagcac ccgggaaacc gttgccacag agacgccagc
    12301 ctaacgcgct acatcacgaa tggtgatcat attcactacc tgttatgaga ttgcagtaaa
    12361 tgactgcttg ctggcggcta ttttgtcagc actgaataca ggacttcgtg aatcgagtca
    12421 cagcaatgga aacggttaca gccgttttgt taacgaatgt gatccagatc gttatcttga
    12481 tgttttagat attgccgttc cggcagcacg caaagttaac tgacgccaga gccattccac
    12541 cggcccctgg cggaagtaac gcagccagat aacagagaag aggatattcg ccagccatac
    12601 cggaataaca aacgccagca gctccaggcg gtcaaaatgc ataaacaaac cgaggtggta
    12661 aaaaagcatg gtacaaatca gcgtttgcaa tagatagttg gtcagcgcca tccgtccgac
    12721 gcaggcgatc gcaagcacca gcttaaagcg gctcaattgc ggccagaagc cataaaacag
    12781 cgacgcatag ccaatcgcct gaaacggcgc actcagttcc cgcggcattt gaagtaagaa
    12841 ggcacaccag cgatacgccc agtccagctg ccattgcagg gcgatggcag gaaggttaat
    12901 ggtcacccca atcgccacca gcacaaaacc agtacgacga tagtgacgta agctgaactg
    12961 ccctttcagc cagccgctgc gcatcagcgc cgcaccaatg agcatcatcc ctgccagttg
    13021 ccagccatac tgtgcgccca gtgccagtaa actgttgcca acaccatcgg cacgattact
    13081 gatcgcttca acgccgccgt gaagcttcca gtatttttca tacaaaatag ccgatgcatc
    13141 cggcgtccag gtgcggctgg tctggctgtc ggaaatcagc cccaataaca gcaaaacgcc
    13201 aagccccacc agataaagca tgacgcctgt attaaacagg ctttttaccg atggcgcatc
    13261 gcgcaccagc cgccagcaga ttaagcccac cagcccgtaa gccagcagaa tatcgccgtc
    13321 ccagaacaat aaaccgtgaa taaaacccag caagactaac agcgttaacc gcgactggat
    13381 ccagcgtctg ccacggggca gcaacatttg caggcccgca ccaaacagca gcgcaaaaag
    13441 cgtgaggaat ttcacctggc cgatgagatc gagaaatgcc caggtccatg catcctgcgg
    13501 cgtaatagcg ccgtaccagg cgggattgag ataagccgcc tttggtagcc caaaggcgct
    13561 gatgtttaat agcaggatcc ccagaatggc gacgccgcga acaaaatcga gcgttacgtt
    13621 gcgctccatg gttcctgcct tttaatcagt tgtgatgacg cacagcgcgc agaaactcgt
    13681 ggcgcgtatt ctgactggat ttgaacaatc caccaagaga ggtcgttgtt gtggcactgg
    13741 ttgcatcgcg gatgccacgc gctttcacgc agtaatgcac cgcatcaatg gagacagcca
    13801 cgttattggt gcccagcagc gtttgtagcg caataagaat ttgctgcgtc agacgttcct
    13861 gcacctgcgg acgctgggca aagaactgca cgatacggtt aatttttgac agaccgatca
    13921 ccgaatcttt cgggatataa gccaccgtcg ctttgccatc gatggtaaca aaatggtgtt
    13981 cacaggtact ggtcagagtg atatcgcgca cggtgaccat ttcatcgacc ttcattttgt
    14041 tttcaatgag ggtgattttc gggaagttgg cgtaatccag accggagaaa atttcatcga
    14101 catacatttt agcgatgcga tgcggcgttt ccatcaaact gtcatcagcc aggtcgagat
    14161 tcagcagctg catgatttcg gtcatatgac cagcaataag gcttttgcgc gtttcgttat
    14221 ccatttcatg cacgggcggg cgcagcggtg tttccagtcc tcgcgcaact aacgcttcat
    14281 gaaccagggc cgcttcttta ctgagtgatg gcatttatga tttctcctgc aggtgtgacg
    14341 cctccgccct gcgtgggggc aaagttatta agctgattta cagcctgatt attgtgcgtg
    14401 aggcggcgca cataatccag tattcacagc gataattatt gtaattgccg ctgactttca
    14461 tcagcagatg ttaaaacatc gttatgcaaa tacggaagtg aaagttactc acagcacatt
    14521 gaataaacgg tatgatgaag aaattgcaaa caacacaaca aggagccacg catggaaatg
    14581 ctcgaagagc accgctgttt tgaaggctgg cagcaacgct ggcgacacga ctccagtacc
    14641 ttaaactgcc cgatgacgtt cagtatcttt ctcccacccc ctcgtgatca caccccgcca
    14701 ccagtgctgt actggctttc cggattaacc tgcaatgacg agaacttcac caccaaggcg
    14761 ggtgcccagc gcgtggcggc agaactgggg attgtactgg tgatgccaga caccagcccg
    14821 cgcggcgaaa aggttgccaa cgacgatggc tacgatttag gccagggcgc aggcttttat
    14881 cttaatgcca cgcaaccgcc gtgggcgacg cattaccgga tgtatgatta tctgcgcgat
    14941 gaattaccgg cgctggttca gtcgcaattt aatgtcagcg accgctgcgc cattagcggt
    15001 cactcaatgg gtggtcacgg tgcgctgatt atggcgctga aaaatccggg taaatacacc
    15061 agcgtttcgg cctttgcgcc aattgtgaat ccgtgcagcg tcccgtgggg aatcaaagcg
    15121 tttagcacct atttaggtga ggacaaaaat gcatggctgg aatgggatag ttgcgcgctg
    15181 atgtatgcca gtaacgcgca ggatgcgatc ccgacgctta tcgatcaggg cgataatgat
    15241 cagtttcttg ccgaccagtt gcaacctgcg gtattggcag aagccgcgcg ccagaaagcg
    15301 tggccgatga cgctgcgtat tcagccggga tatgatcaca gttactactt catcgcctct
    15361 tttatagagg atcacctgcg cttccatgcg cagtatttac tgaagtgaaa gtccgcccgg
    15421 ttcgccgggc atcttctcat cagaagcgat aatccactgc cataaagtaa cgacgtccgt
    15481 cttcgttata gctgtagtcg tcacgactga gatccttgtc gccaaggttc agcacgcctg
    15541 cgcgcagttt gacgtcttta gtcacctgcc aggccgcgcc ggtattccag atggtataac
    15601 cgcccggtgt tttagccgtc gcgctgtcgg cgcgtttctg cccggtatag tgaccagaaa
    15661 catagaatga ccagtcttcc agcgccagcg gtttccagtc cagcgtaccg ttagcagtat
    15721 ggaacggcag atcggatagc ggtttgtttt cgccgttgct gacatcacga ccatcgttgt
    15781 aggtgtagtt gatcgacagt ttccattcat cgttgaacgg aattttcagt tcggtttcca
    15841 cgccctgaat acgagctttg ttaacgttgt agtagctaaa taccggtatg cgccgtccgt
    15901 tagcgcccgt ctcaaaacca acaaagtttt ggtagcccgg tgcagcgttg acgtcagacg
    15961 tacggctgat gctgatacga tctttcacat cgttacggaa aacggtaacg ctggattcaa
    16021 ccccttccag ccagccttct tcacccatgt agtaaagccc cagctcccaa ctttcgctgg
    16081 tttctggttt cagatccggg ctacccacaa tcttacatgc gccacggcag gaattgctcg
    16141 tccagtcagg gctaagttgc aacagagaag gtgctttaaa tgccgtcgcc cagccccctt
    16201 tcaccgttac ggtgtcggtg gcgttataaa ccaggtaggc acgcggactc cagtgttcac
    16261 cgtaggtttc gtgatcgtcc atacgcacgc cggtcgtcag cgccagcggc tcgaagatcc
    16321 gccattcatc ttccacaaac agcgcgtact ggctggcaga cgttttggag ctggttcccc
    16381 cggtcaggtt caccgcatcg ctaagtttgt cgtgacgcca ttcaccgcca accgtgagaa
    16441 actgattaat cgccgtcagc ggcaacgtgt atttgccgtc gaccgtattg ctttcggaag
    16501 ttatcgggct gctgttgcca gggtttttgt tctcgacttt ctcaccgtag tatttcagtt
    16561 cgctggtgcc gtaatcccaa cgcccattat ggctgacgga gtagttctgg cgttccaggc
    16621 ggtttttgtc cagcgagtcg gaatcacgat cctgacggtc gaaaccgtat ccggcagtaa
    16681 aatcgtgatt ttgattcggt gtccaggcaa attcgacatt gccgtcgcgg ctggagaatc
    16741 cttcaatacg cggcgtttct ccggtatcgg tggtcgttga gttttgcggg tcatcctttt
    16801 cacgttttgc caggctgccg taagctttca ttcccagcac accatcaatt aatggtccac
    16861 tggtaaagaa ctgaccgtta taggtatcac cgcgatcgcg atgttcctga atggtggtat
    16921 cgacggtaac ggtacccgac catttctgac cgattttttt ggtgatgata ttcactacac
    16981 cgccgagcgc atcggaaccg tacagcgacg acatcgggcc acggaccact tcaatacgtt
    17041 cgatggaatc gaccgggatc cagttcagat cgaaatcatt gtggcggaag acggcattgc
    17101 gggagttcac gcgtttaccg tcgacgagaa tcagggtata gctgctgtcc agaccacgaa
    17161 tactaacgcc cttacggtta tccccttcgt tcgtcagttg tacgccaggc acttctttga
    17221 ggacatcctt cagattctgt accggttttc gctgcagatc ttcctgggta atgacgctga
    17281 tactggcggg tgcatcttta aggttttgtt caacggaaga tgcagtgaca accatcgttt
    17341 cgccatcgtc atcgaccgct aacacaggcc atacacaaga aatagcggac aaacacagcc
    17401 cgacccgtac gacagggttc aacctaaaca ttccatatct ccatgaggta actacgaaaa
    17461 taaaatgggt atcgctcaca tcttctttac gtccccttgc gtacggcagc atcgcggtgg
    17521 acttatttgt atgcaggtga tcatcccaga aagccggacc agcctctgat ttgtcagcct
    17581 tttttgattg tagcgtaacg ataatgcaaa cgataacaat tatcaatcca aatgttaaat
    17641 tttatatccg catgatttgt cagggaaatg gaattgaaaa agccctctcg gttgagaggg
    17701 cttagcaagg aagggaggaa acttatttct tatcgttctg cgggaacttc atttcgctgt
    17761 agcgtacgaa gtgagttcct ttaatcagct tgtagccgaa ccaaataatc aggaacagcg
    17821 ggataccaat atacgttgcc gctacgccgc cccagtcaat ggtatctttc aggaacgctt
    17881 cgtagttctg gcccaaagtg ataatcagac acagaatgaa tgcgaagatc ggccccagtg
    17941 ggaagaaacc tgaacggtac ggcagatcgt taatgtcgtg tccctgcaat acgtaaccgc
    18001 gacggaagcg atagtggcta atggcaatcc ccagccaggc gataaaaccc gtcatcccgg
    18061 aggtgttcag cagccacagg tataccgtct ggttgccaaa catggaggtc aggaagcaca
    18121 aaccggcaat caccgtcgtc gcatacagcg cattacgcgg cacgccacca cgcgacaatt
    18181 tagcgaaaat gcgcggcgct ttaccgtcac acgccagggt gtacagcata cgagtagacg
    18241 catacatacc ggagttacct gctgacagca ccgccgtcag aataactgcg ttcatcaccg
    18301 ccgccgcaga gagcagaccc gcgtgctgga acaccaaggt gaacggactg acgctgatgt
    18361 ctttaacatc gttacgcagc aggctcggat cggtatacgg aataatcagg ctgataatca
    18421 ggatcgcgaa cacatagaac aacaggattc gccagaatac ctgacgtacc gcgcgtggaa
    18481 tgtttttcgc cggatcttcg gactcgcctg cagcaatacc gatcagctcg gttccctgga
    18541 aagagaagcc gacaatcata gctacgccga tcatcgccgc aaaaccacca gcaaacggcg
    18601 cttcgccgat tgtccagttg ctccagcccg caggctgcgc gcctttgaag ataccgataa
    18661 tcatcagcac gccaacgatg ataaagacaa taactgtcgt gactttgatc agtgaaaacc
    18721 agtattccgc ttcaccaaag ccacgaactg agatgtagtt cagcaggaag ataacgccga
    18781 ggaacaacgc actccagatc cagcccggtg tatccgggaa ccaccagctc atgaccagct
    18841 gtgctgcaac caagtcaacg gcaatagtca ccgcccagtt gtaccagtag ttccagccca
    18901 gcgcgaagcc aaagccttct tcaacatagt tctgaccgta agtggcaaac gaaccggaaa
    18961 ccggcatata tgcagccaat tcaccgagac tggtcatcag gaagtaaacc atcaggccaa
    19021 tcagcatata cgagagcaat gccccgcccg gacctgcctg agaaatcgtt gcgccagagg
    19081 caacaaaaag acctgtaccg atggaaccgc caatggcaat catcgtcagg tgacgcgcct
    19141 ttaattcacg gcgtaagccc ggcgcttctg tagttttagt ttcggaaccc atacaaaaat
    19201 gctatccatc ttaaaaatga ggcgcgattg tagcagacga ttggcaatcc ttccggcaga
    19261 aatacacggt tataagacac cttcatgatc gcccagggat tataagtaaa gcagccaatc
    19321 tcttttctgg cgaatgcatg ctgagggcgc tgcgtcacga aatcgacaca gcaccagcat
    19381 gttcttgtac agcaccttaa cgcggtacat tcgcgggatc gcaatagtcc agaaagcgcc
    19441 gtagcgcgtt ggaaaggtgt ttttgccgat gatgtatacg ccacaacgta cgcatcaggc
    19501 gcgggagagg gaccgcaact tcacttaatg tgcctgcctg caattgatct tcaatcacac
    19561 gtcgcgacag gcaactaatt cccaacccat gacgcaccgc atgtttgatt gcctcggagt
    19621 tacctaattc catcgccatc tcaaacttcg gtaaatgtga cagcaacaga taatcgacaa
    19681 tctcccgcgt gccggaaccg cgttcacgca ggatccacgg cgcagcggcc agctgttcta
    19741 aggtgaccgg accacgagcc aacggcgaag tcggcgcggc gaaaaccacc agctcgtctt
    19801 ccagccacgg ttcagaaatg atttcagtgc tgtggcacgg tccttcaata aagccaatat
    19861 caacgcggaa atccagcacc gcttgcatca cgtcctggct gttcccaacg ctaagttcaa
    19921 tcggcaactg cggataatca tggcgataac gggcgataac tgcaggcaga atgtagttac
    19981 cgatggtact actggcatag atacgaatcg cgccgttgtc ttcgcgaaac agttgttcga
    20041 tttcaaccgc ctgttcaagc aatgccaacg cacgcggata gagcagccgc ccgtgttcat
    20101 taacaaccag tcttttcccc acgcgatcaa acagttgcac gccaagctgc ccttccaggt
    20161 cggtcaaggc tgcgctcact gctgattgcg acaacgccag catcaccgac gcctgggtgg
    20221 ttgatccact tttcaatact tctgcaaaaa cttccaactg ccggagggtg atgtgcatag
    20281 tcgcttacca cttataaaga ttaattataa atatataatc aattttattt ttaaaccagt
    20341 tagtcgtaac cttatgcccg gttaaaggag agggttatga cgaatatcac cttacagaaa
    20401 caacatcgta cactgtggca ttttattccg gggttagccc tgagtgcagt tatcaccggg
    20461 gtcgccctgt ggggtggttc cattcccgcg gttgcgggtg ccgggtttag tgccctcacc
    20521 ctcgcaatct tgttggggat ggttttaggc aacaccatct atccgcacat ctggaaaagc
    20581 tgtgacggtg gtgtgctgtt tgccaagcaa tatttattac gtctgggtat cattctttat
    20641 ggcttccgtc tgacgttctc gcaaattgcc gatgtcggta tcagtgggat catcattgac
    20701 gtgttgacgc tgtccagtac cttcctgctg gcttgcttcc tggggcagaa agtgtttggt
    20761 ctggataagc acaccagctg gttgatcggt gccggtagca gtatctgtgg tgctgccgcg
    20821 gtactggcga ctgagtcggt agtgaaagcg gaagccagta aagtaaccgt ggctgttgca
    20881 accgttgtta tcttcgggac cgtcgcgatt ttcctctacc cggcgatata tccgctgatg
    20941 tctcaatggt ttagtccgga aactttcggt atctacattg gttctactgt gcacgaagtg
    21001 gcgcaggtgg tggcggcagg tcatgccatc agcccggatg cggaaaacgc agcagttatt
    21061 tccaaaatgc tgcgcgtgat gatgctggct ccgttcctca tcctgctggc ggcgcgtgtt
    21121 aaacagctgt ctggggcgaa cagcggcgag aaaagcaaaa tcactattcc gtggtttgcc
    21181 atcttgttca tcgtcgttgc catctttaac tcgttccacc tgttaccgca gagcgtggtg
    21241 aacatgctgg taacgctgga taccttcctg ctggcaatgg cgatggcggc gctgggtctg
    21301 accactcacg tcagcgcgct gaaaaaagct ggggcgaaac ctctgctgat ggcactggta
    21361 ctgtttgcct ggctgattgt tggtggtggt gcgattaact atgtgattca aagcgtcatc
    21421 gcataaacca ctacatcttg ctcctgttaa cccgctatca ttaccgtttt cctccagcgg
    21481 gtttaacagg agtcctcgca tgaaatacat tggagcgcac gttagtgctg ctggcggtct
    21541 ggcaaatgcc gcaattcgcg ccgtcgaaat cgacgcaacc gcgtttgcct tgttcaccaa
    21601 aaaccaacgt cagtggcgtg ccgcaccgct cacgacgcaa accatcgatg aattcaaagc
    21661 cgcctgtgaa aaatatcact acacatcggc gcaaattctt ccccacgaca gttatctgat
    21721 taacctcgga catccggtca ctgaagctct ggaaaaatcg cgcgatgcct ttatagatga
    21781 aatgcagcgt tgcgaacaac tggggctttc tttgctcaac ttccaccctg gcagccatct
    21841 gatgcagatt tcagaagagg attgccttgc gcgtattgcc gaatccatca acattgcgct
    21901 ggataaaact caaggtgtaa cagcggtgat tgaaaacacc gccggtcagg gcagtaactt
    21961 agggtttaaa ttcgaacatc tcgcggcgat tatcgacggc gtggaagata aattccgcgt
    22021 cggcgtctgc attgatacct gccatgcttt cgctgccggg tatgatttgc gtactccagc
    22081 cgaatgcgaa aaaacgttcg ctgattttgc ccgtattgtc ggctttaagt atctgcgcgg
    22141 gatgcacctt aacgatgcga aaagtacctt tggcagccgc gttgaccgcc atcatagcct
    22201 cggtgaaggc aatatcggtc atgatgcgtt ccgctggatc atgcaagacg accgtttcga
    22261 cggcattccg ctgatccttg aaaccatcaa cccggatatc tgggcagaag agatcgcctg
    22321 gctgaaagcg caacaaactg aaaaagcggt ggcctgatcg atgaacgaca gggaaaagca
    22381 gattcttaaa attttacggc gtaacccgct gattcaacag aatgaaatcg ccgatattct
    22441 gcaaatcagc cgctcacgtg ttgccgctca tattatggat ttgatgcgca agggtttgat
    22501 taaaggcaaa ggatatatcc ttaccgagca ggattactgt gtggttgtgg gtgcaatcaa
    22561 tatggatatt cgcggaatgg cggatatccg ttacccgcaa gcagcctctc atccaggttc
    22621 tgttcactgt tccgcaggcg gcgtcggacg taatatcgcg cataacctgg ctctgcttgg
    22681 tcgggatgtg catttaatat cagctattgg caacgatttt tacggcgaga cattactgga
    22741 agaaacgcgc cgcgcgggcg tgaacgtttc aaactgcatt cgtctgcatg gacacagcac
    22801 tgcaacttat ctcgctatcg ctaataagca ggaagaaact atcctggcaa tcaatgacac
    22861 acatattctg caacagctaa cgcctcaact gctcaatact tcacgggact tgattcgcca
    22921 tgctggtgtg gtgctggctg attgtaatct cacacctgaa gccctggagt gggtctttac
    22981 cattgctgat gaaattccga tgtttgtcga taccgtttcc gaatttaaag cgaacaaagt
    23041 aaaaaactgg tacagccgga tccacactct gaaaccaaca caaaatgaac tggaaatttt
    23101 gtgggggcaa ccgattaaag acgacaacga tcgcatcaga gctgtaaatt cgctgcatca
    23161 acaaggtgtt aaacgcattt ttgtttatct gaaagatgaa tctgtatttt gcagcgataa
    23221 ggatggagaa caatttttgc tgaccgcgcc agcgcatacg acagtagaca gttttggtgc
    23281 tgacgatggt tttatggcgg gcctggtata tagctttctt gaaggataca gtttccgcga
    23341 cagcgcccgt tttgcggtag cctgcgcggc aatttcgcgc gccagcggca gcttaaacaa
    23401 ccctaccctg tctgccgata acgcgctttc attagtgcca atggtgtaac aatgttgccg
    23461 gattcgacgc ttgaccgcat cttatccggc tcataaattg cgcactacgc taaaccaata
    23521 aagaatcctg caatagtagc actcatcagg ttagaaagtg tcgccgccgc cagcgcgcgt
    23581 aaaccaagtt gggcgatttc cggcgcacgg tgtggcgcaa ccgcagaaaa cgcccccacc
    23641 accaccccga tagaaccaaa gttagcgaaa ccgcacaacg cgaaagaaat aatcgccacg
    23701 gttttagcat ccagagtgcc acccgtttgc agatatggcg agaaattgag ataagcgaca
    23761 aattcattga tcgccagctt ctgcccaatc aaactcccgg caagatttgc atcactccag
    23821 tcaaccccca tcacccacgc caatggggcc aacaggtaac ctaaaatcga ctccagcgaa
    23881 gcatgttcaa aaccaaacca gccgccaaca ccgccgataa taccgttaat caacgcaatt
    23941 attgcaacaa atgccataac aactgtcgct acaccggcgg cgattttcag cccggtcatt
    24001 gcccctgtcg cagcggcttc aataatgctt tttggcggtg tttcggtgaa agagaggtta
    24061 ttaaaagaaa cctgcgaaga ttccgtagcc gggcttaaca ggcgggcaaa caagatcccg
    24121 ccagggatcg ccattaacga tgccgccagc aaatattcca caggtacgcc cagtgcggcg
    24181 taaccaatca ttgtcgaacc agcaatcgag gccatgccac tacaaatcgc tgtaaataat
    24241 tcattgcgat tcagacgatc gataaagggt ttgacgatcg ccggaatttc gttttgcccg
    24301 aggaaaatgg tggtgaccgc gacgaatgac tcgatcttgc tgatatttaa agctttctgg
    24361 aagataccgc cgagaatgcg aattaagatc cccatcacac cgatgtagta gagaatactc
    24421 accagtgcag tgacgaagat aattgccggt aataccctga aaccaaagat aaatcctgcg
    24481 ccatcaaata aggtatccat tttcggtccg accagagaac cgaagataaa tgcgctacct
    24541 gcgtcgctgt acgccatcac tttgtggacg ccaaaagcga ctttttctgc gacccaacgc
    24601 cctggcggta accaaagcat aatcccgcca atcacgacct gtaacactaa cgccgcgcca
    24661 acggtacgca ggctgatctt cttcttgttt actgacagta aaaacgcaat tgccagcagt
    24721 accaccattc ccagaacact tctcatgaca tccatagtga ttatctcttc atgtcatttt
    24781 gggcgacggc tttctgccca tcagaaatag ccgggcctgc atcttgcgat actgtcccgg
    24841 cttgttatca acacttaatg ggtttttatg tagccgcgta cgcactcttc gactaatccc
    24901 cagaaccagt cggtatcaat agtgatgccg actttagtat tggcgggctt gccaagaacg
    24961 cccagctcgt cgcagacggt acgcccatag caagggccac tgttgacgtc cacttcgacg
    25021 tacatctcct gggttttaat gccatcaggg ttaatcagat aaccgatgca ggtggcgtcg
    25081 tgcaccgggc cgccagccag gccgtagttt tcgaactgcg ttttgagagt gaagttcatg
    25141 atgtcgctga acatctctcc ggcagggccg cctgcccttt ccattcgtgc aattacgtcc
    25201 ggggtgcaaa cggtctggtt ggtaagatcg aggcccatca tcactaatgg aacgccggag
    25261 gtgaacacca cgcgtgcggc ttccgggtcg gcaaagatat tgaactcggc agatggggtg
    25321 aagttgcctg taccgtaagc gccgcccatt agcacaattt cacggatttt gggcaggatt
    25381 gcgggttgca tacgcattgc caccgcgata tttgaaagcg gaccaaccgg caccagagtg
    25441 atatcgccat cgcttgccat aagggtatca atgatgtatt tcaccgcgtg ggtactttct
    25501 gcctggcggg tcagcggctc gaataccggg ccatccagtc cggtttcacc gtgaatatta
    25561 tcggcaacga tttgttgacg cataatgggt tgcggcatcc ccgcataaac cggaacatta
    25621 atctccagtt tctggcaaac attcaggcca ttaattaatg ttttatcaag cgtctgatta
    25681 cccgcaacaa tggtgatacc taataaatca atcgccggat gtttcgccgc catcattata
    25741 gcaatagcat catcatgacc cggatcacaa tccagaataa tttttctctt ttccattgtt
    25801 tatttcctct gtttccagtt gcgttatttt ttctacagca acgaaatttc gatcgggccg
    25861 atgattgaat cttaacaaca gcgtacgaat gctaaatatg agaaatctca tagcggataa
    25921 acatcgtgaa agaaatccac aataatgatc ttaagcagca attgatgagt gaatccgcgt
    25981 ttaaggattg ctttttaacg gatgtttcag ccgatacgcg gctgtttcat tttttagcgc
    26041 gtgactacat tgtgcaggaa gggcaacagc cgtcctggct gttttacctg acgcgaggcc
    26101 gcgccaggct ttacgccacg ctagctaatg gtcgcgtgtc gctgatcgat ttctttgccg
    26161 ccccctgttt tattggcgag attgagttaa tcgacaaaga ccatgaaccg cgtgcggtgc
    26221 aggctattga agagtgttgg tgccttgcgc tccctatgaa acattaccgc ccgctgttat
    26281 taaacgacac gctattttta cgaaaactct gcgtcacctt aagtcataaa aattatcgta
    26341 atattgtttc tttaactcag aatcaatcat ttccgttagt gaatcgcctg gcagcattta
    26401 tattactctc gcaggaaggt gatctttatc acgaaaagca tacgcaagcg gcagagtatt
    26461 taggcgtttc ttatcgacat cttttatatg ttctcgcgca attcattcac gacggtttat
    26521 taattaaaag caagaaaggg tatctaatta aaaacagaaa gcagttgtca ggactggcgc
    26581 tggagatgga cccggagaat aaattctccg ggatgatgca gtaaaaatta ttggcaatag
    26641 cacgattgcc ggatgcaacg cttaataagg cgtacatccg gcaatgaaac tcaagctaaa
    26701 ccaataaaga acccggcaat ggtcgcgctc atcaggttgg aaagcgtcgc cgccgccagc
    26761 gcccgtaaac caagctgggc gatttccggc gcgcgttttg gcgaaatagc cgaaaacgct
    26821 ccaacgacaa caccgataga accaaagttt gcaaacccac agagggcgaa cgagataatg
    26881 gcaatggtct tgacatccag cgtgccgctc gtttgcagat agggcgagaa attgagataa
    26941 gcaacaaact cattaatcgc cagtttctgc ccaatcaaac ttccggcaag atttgcatca
    27001 ctccagtcca cccccataat ccatgccaac ggggccagca catagccgaa aatgctttcc
    27061 agagagacat tggcaaaacc aaaccagccg ccaactccac caataatgcc gttgatcagc
    27121 gcaatgatcg ctacaaatgc cattaccacc gtcgccacac cggcggcgat tttcagcccg
    27181 gtcattgcac cgttcgccgc agcttcaata atgctttttg gcggtgtttc gctaaacgac
    27241 agattttcaa acgtaacttg cgaaggttcc gtcgccggac taagaatacg tgcaaacagg
    27301 ataccgccag gaatagccat cagcgaagcg gctaacaaat agtcaatcgg cacccccatt
    27361 ccggcatagc caatcatcat cgaacccgca atagacgcca tgccgctaca aatagccgta
    27421 aatagctcgt tgcgattcat gcgatcaata aaaggtttaa cgatcgccgg gatctcattt
    27481 tgcccgagga aaatagtggt gactgcgaca aaagattcga ttttgctgat gttgagggct
    27541 ttctgaaaaa tactgccaag aatacgaatc agcagcccca ttacgccaat gtagtacagc
    27601 aagctgataa gcgcggtaac gaaaataatc gccgggagta cgcgaaaggc gaagataaag
    27661 cccgcaccgt caaacagaac atccattttg ggaccgacca gcgaaccaaa aataaaggca
    27721 cttcctgcgt cactgtagga catcacttta tggacgccta atgccgcctg ctctactgcc
    27781 cattttcccg ggggaaaata gagcatgatg ccaccaatgg caatttgcag cagcagcgca
    27841 gcgccaaccg tgcgcaagct gatgctcttt ttatttaccg acaacacaaa ggcaattgcc
    27901 agcaatacca ccatccccgc aacacttctc attatatcca taatgatttt cccttcatgc
    27961 cagtaaaccc ggcgtcagcg ccaggttttg gtatgtttga tgagtacggg cgacggcttt
    28021 ctgcccgcca gaaattaacc cacgagacgc tgatattctt tggcaatttc gctcgccaga
    28081 atggcgttat taaagaccag ttgaatattg gatttcaggc tatcaccgcc ggttagctcc
    28141 gcaacacgcg ccagcaggaa cggtgtactc tctttcccaa taacaccctg ttcttcggct
    28201 tcagcaacgg cttgatcaat tgctgcattg atggtttctt ctggcatcgc aaactgttcc
    28261 gggatcgggt tcgctaccac caggccgccg ttcaaaccgg tttgccactt cactgccatc
    28321 gcgcgagcaa tttcgcgggc actgtcaaga cgaatgctga cttcaaatgg gctggttcgg
    28381 cagaaaaacg caggcagcgc ttttgtctga taaccaatta acggtacacc gaaggtttct
    28441 aaatattctg ttgttaatcc taaatcgaga atagattttg cccccgcaca aacaacagtg
    28501 acattggtat ttgccagttc ctgtaaatcg gcagaaatat cgaaggtatg ttccgctccg
    28561 cgatgcacac caccaatacc tccggttgca aaaactttaa tcccagccag tgctgcaata
    28621 atcatggttg atgcaacggt agtcgcgcca tttttccctg cagcaacgac aaaaggtaaa
    28681 tcacgacgac taactttcgt cacgttatgt ccttcacggc ccagcaattc gatttcttct
    28741 ttacttaagc ccactttcat cacaccgcca ataatggcga tagtggctgg tactgcgccc
    28801 tgtttacgaa ttgtttcttc tacttcaatt gcggtttggg cattttgtgg gaatggcatt
    28861 ccgtgagaaa ttatggtcga ttccagcgca acaaccggtt ttttattttt taaagcatcc
    28921 tgtacttcag cggatatttg taataattca ggggacagag ttaattcaga cattctgtgt
    28981 tctccactaa cgatctaaca ttggcaattg ataattcagg gttgttggta tattcacagg
    29041 caagtgccat tgacgaacat ccctgcgcaa atcgaacaga atcgataaac ggcataccgt
    29101 ctacccaaca ggaggccagc cccgccatca tggcgtcgcc tgccccagta acattgatga
    29161 cattcgtttt aataggggcc gaccacccac tttcaccgtt gatatcactg tagtaaacgc
    29221 catcccctcc catgctcagc accaagcggt tcaggccgtg ttgatgaaac caggcggcga
    29281 cctttgcgac atcttcacgt ccggaaagtg caattccgct caacgtttcg gcttcaagac
    29341 ggttaggttt taacgtatgg attttgctca gatgatcgcg gattttgacg catttccatg
    29401 ccgataccgg atcaataaat acaggcgttt ccccgctatt ttccagaacc cagaccagca
    29461 cctcttcact aaggttgcaa tccgcgacaa taacctttgc accgcgaata aactcctgat
    29521 gctgggcaag aaattctgca gaaatacagt cactaatact catatcattt atcgcgacaa
    29581 gcatttctcc tgtattatcg agcagtgata aatagctgga tgtattctca cccggaacaa
    29641 ttaagcattt atcgacataa acaccggatt gattagtttg cgccagtaat gattgcccat
    29701 aaaaatcacc acctactgca cttaaaagcc aggcattttt ccccagtaac gccagattat
    29761 gagcgatatt ccttcccacg ccacctgggg taaatttaat ttttccggga ttcgaatccg
    29821 cataattcaa tggatgatgt gaatacccag ccacatcaat attcgccgaa ccaataatta
    29881 ctatgtagtc cttttcgcgc atagcacgcc ccctggcaat aaattgccaa cgaagaaatt
    29941 gggtaaaata gccattacag tcaataagac tattagagca cgtgttcaga tttaaacaca
    30001 tgttcataat aagcatattt aacgtaatgt aaatcgatca gaaaaaacga aatcggccag
    30061 atatgaaggc ggtcacaaag tttgtctatt tatgcagcaa caaaagagaa agttgtgatg
    30121 tgtaacacac aatatggcag gcaagaaaaa gggcagagat gtttctgccc tccagattgt
    30181 tgacaacgcg cgctttgttc atcctggatg tggcgtaaac gccttatccg acctacaaaa
    30241 gcgcaaaaaa ttcaatatat tgcagggttc atgtaggtct gataagcata gcgcatcagg
    30301 caattttgcg tttgtcatca tcggggcaga gatgtctcat ccccgataat gaagataaat
    30361 tatgcgtttt tttctactat ttgtgtttcc gggcgtttca ggaaggcata ggccaaaccc
    30421 gccaccagcg taccggcaat aattgctacc aggtaaccca ataccggcgt aatagcgcca
    30481 gggatcagca gaacaaacag accaccgtgt ggtgccatca gtttcgcacc aatcgccatt
    30541 gagattgcgc cagtcagcgc cccaccaacg atacagcacg gtagcacacg catcggatca
    30601 cgagcagcaa acggaattgc accttccgaa ataaagcaca gaccaagtac cagagcggct
    30661 ttgccacctt cctgctgcgc tttgtcgaat ttgcgacgcg ccaccattgt tgccagaccc
    30721 attgccagcg gtggcaccat acctgccgcc ataatcgccg ccatcgggcc ataggtttga
    30781 gtactcagca gacccacacc gaatgcgtac gctgctttgt ttaccggacc gcccatgtca
    30841 gtacacatca tgccaccgag gatcgccccc agcagaaccg cattcgcagt ccccatggtc
    30901 tgcagccagt gagtcaaccc atcgagaatg ccagcaaccg gtttaccgat taggtagatc
    30961 atcgccagac cgaccaccag actggaaatt agcggaatga tcaggatcgg tttcagcgcc
    31021 tccatactct gtggcagttt caattgcgta ctgattaact tcgcaatgta accagccagg
    31081 aagccagcaa ttataccgcc aataaagcca gaaccggtgc tgaccgccag cataccgcca
    31141 atcagacccg gtgtgaggcc cggacgatcg gcaatggaga aggcaatata acctgccagt
    31201 accggcacca tcagcgcaaa ggctgaacca ccaccaatct gcatcagcgc cgctgccaac
    31261 gtacccggct ctttaaacgc ttcgatacca aaagcaaaag aaagcgcgat acacagacca
    31321 cctgcaacaa ccatcggcag catgtaagag acgcccgtca gcaagtgacg gtaagcgcct
    31381 gcactctctt tcttaccttc agtggtcgct gtttgagctt tgcccgctgg ttcatacggc
    31441 gttgcttcag caaccgcttt atccagttcc tgcgcagttt tcttcagcgc cagaccggta
    31501 gaggtgcgat acatcggttt accagcaaat ttcgccagat ccacttcgat atctgccgcc
    31561 acaatcacca gatccgctgc tgcgacttct tcaggggtga ttgcattgcc cgcgccaaca
    31621 gaaccacggg tttcaacttt cacccaccag ccacgttttt tcgcttcggt ttcaatggct
    31681 tcagccgcca taaaggtgtg tgctacgcca gtcgggcaag cagtcaccgc aactacgcgt
    31741 ttcggaccgc tggcagcaac cggtgctgtc gcagcgaccg gcgcagtgta aggtttcgca
    31801 tggcctttgg cttcactcag gaacagctca gggtgcgcaa ctgcccggga aatatcaccc
    31861 agccagacat ttttaccgtt cagcgcgctg tcattcggga tggaatcacc gagaacaatc
    31921 gccatttctg cgtcgttcgg attgtcgatg atttccagtt ttgcttttcg cgccgccgcg
    31981 cccagcaggg tcttcgccat ataggcgcgt gcctgaccga gattagcgtc aataatcagc
    32041 agcgttttca ttatgcctct cctgctgtca gttaaaaggt tgtaagtcga cgcgcgccat
    32101 cattgcggcc aactgcggac gatcggtaat acccacattg ctttgactta ccgccagggc
    32161 tgcaacagct gtcgccagac gcagtgtgtg ttcactggat tcacgcatca gcaagccata
    32221 aatcaggcca ccaaccatag aatcccctgc gccaacggtg cttacgacat cgactgacgg
    32281 tggtttggcg atccattcgc cggaggcatt aacccaaagc gcgccttcgg cacccagtga
    32341 aataacaaca tgcgcaatac cttgttcacg tagcgcatgt gcagcttcaa tcacatcttt
    32401 catttcaggc agtttacggc ctgcccagat ttccaactcg cggcggttag gtttcaccag
    32461 ccacggtgcc gctttcaaac ctgctactaa cgcttcacgg ctactatcaa agataatgca
    32521 aggacactga ctacgcaggc gagtcatcca gtcggtgaac gcttccgggc tgacgcctga
    32581 cggtaagctt ccgctgacac agaccatatc gaactgaccg agccagctca gagaatcagt
    32641 cacaaagcgt tcccagtcgg cgagggtgac ttcaaaaccc gagaagttga agtcggtcac
    32701 ttcgccgtct ttttccgtca gcttaacgtt aattcgggtg cgcccctgta caacctggaa
    32761 acggttggca atgcccagct cgctgaacag ttgctgaaaa ccatcctgat tgtctttacc
    32821 caggaagccg ccaacggtga catcaattcc caggtctttt aatactttgg ccacgttgat
    32881 gcctttaccc gccgcatgca gaccggtggt tttcaccagg ttcacttcgc cgcgttcaat
    32941 ttccgggcag aaaccaacaa ggtcataagc cggattaagg gtgatcgtag caacacgtct
    33001 gctcattatg cgccctcccc aagaccagca gcgatagcgt cgccaatggc tttcagcgcc
    33061 tgttcagcat ctgcaccctg ggcggtaaag cgtaggcgat gacctttctt aacgccaagt
    33121 gccacaactt tcatcagact acgtccgttt gccggtttgc cggtgccatc aaggtttgtc
    33181 acggtaatat cactgttaaa ttgtttaatg gtattgacca gcatggtacc tggacgagca
    33241 tgcaggccgt gttcattgcg caccacaaac tccgcgctta acacgtcgtc ggtcggcgca
    33301 tcatcgctgg tcagcagcgc cagcaacgtt gccgcatccg ctttcagcaa gcggtcagct
    33361 ttattgtcga gcaataaatc agcgagacgc ttaagaaccg cgatgggctg atcgtcattc
    33421 atcgccacac tcaccagcat ggctgccgtt tcgccgtcca catcaaaagc atttgccgca
    33481 cggcttaccg caatcgcgct acgcagattg ccttcggcgc tatcgctcag ccagataccc
    33541 tgtccgagat tcagcggttg ttcattgatg gctttggtga cgaaagtggc gtcaactgcc
    33601 cccacctctt tcagacgcgc agcgttcagc gcctgaagag tcagcagatc gctggcgacg
    33661 atatccagcg tcagcatttc gttgtcgagc ttcagctgct cactctgctt ttcgcccatc
    33721 agtaatgcgc gaagttcttc tgctgttgtt gctgacttca gttgttcagc aacggaatca
    33781 tcgctcagta cgtgggtcag ctggcgtagc aggcccagat gttcatccga gctggcagca
    33841 ataccgattg ccacgtacgc tacctgaccg tcaccccagg tgacgccttc cgggaactga
    33901 aatacctgaa cgccggtttt cagcacctga tcgcgggtgt cggtagtgcc gtgtggaata
    33961 gcaataccat tgccgaggaa cgttgaggtt tgctgttcgc gcgccagcat gccattgacg
    34021 tagccttctg ctacattacc ggcctgcacc agcgccgcag cgacctggcg aatcgcctct
    34081 tctttgtctc cggccttttc gcccggatgg atgtcctgta cggataactg gaacatagtt
    34141 ctcctctctt gctgaattga aacgattcag cctctatgag aaaaaaagcg ccaacctggc
    34201 ttagggttaa agacaagatc gcgctgaaac gtttcaagaa agcataatac ttctgtttca
    34261 gcacgcaagg aaagccgtaa agttatgagc aaaagtttga tgtgctgcac aattttttgt
    34321 catttttcct aattgctgat gggaaaatcc gttatcagca atttcatttc agcagcgtca
    34381 gcagccaatc agcaacagtg aaatggcatt ttgattattc gtggcgcttt tgatttcgct
    34441 gtctgtttat tttctgacaa gcagcgtaaa ctccgcgtct tcctcttcca gtgatcgacc
    34501 tgcatgcata actcccccgc agtctccagc gcgaaatcgt ttgacctgac ctcgacggcg
    34561 tttttaatcg ttgcctttct caccggtatt gcgggtgctc tgcaaacccc gacactcagt
    34621 attttcctta ccgatgaagt acatgcccgt ccggcgatgg tgggattctt ctttaccggc
    34681 agcgctgtca ttgggattct ggtaagtcag tttctcgctg ggcgctctga taagcgcggc
    34741 gatcgcaaat cgctgattgt cttttgctgc ctgttaggcg tgctggcctg cacccttttt
    34801 gcctggaatc gcaactactt tgttttgcta ttcgttggcg tctttcttag cagctttggc
    34861 tcgaccgcta acccgcaaat gtttgccctt gcccgtgaac atgccgacaa aaccggacgt
    34921 gaggcggtga tgttcagctc ttttttacgc gctcaggttt cactggcatg ggtcattggc
    34981 ccaccgctgg cttatgcgtt agcgatgggt ttcagcttta cggtaatgta tctgagcgca
    35041 gcggtagcgt ttattgtttg cggcgtgatg gtgtggctgt ttttaccgtc gatgcaaaaa
    35101 gagcttccgc tggcgaccgg cacggttgaa gcgccgcgcc gtaaccgtcg cgatacgcta
    35161 ctgctgtttg tcatttgtac attgatgtgg ggctcgaaca gcctgtacat catcaacatg
    35221 ccgctattta ttatcaacga actgcatctt cccgagaaac tggccgatgt gatgatgggg
    35281 accgccgccg ggctggaaat cccgacgatg ttgattgccg gatatttcgc caaacgtctg
    35341 ggtaagcgtt tcttaatgcg cgttgctgcc gtgggtggcg tctgttttta cgcaggaatg
    35401 ctgatggcgc attcacctgt cattctgttg ggcttgcagc tgctaaatgc tatttttatt
    35461 ggcattctgg gcggcatcgg gatgctctat tttcaggatc tgatgcccgg tcaggcgggt
    35521 tcagccacca cgctctatac caacacttcg cgcgtgggct ggatcatcgc aggatcagtg
    35581 gcgggcatcg tcgccgaaat ctggaattat cacgctgtgt tctggtttgc gatggtgatg
    35641 attatcgcca ctctgttttg cttactgcgg attaaagatg tttaaggcgc ggtcagcatt
    35701 tccagatcga taagccaggt catggcctgc tgacgcgagt taccgcacat ttcagcgctg
    35761 gcttgtaagc ctgcacagac tttcggtcgc agtggcgagg tgaaaatttt acagcgttgc
    35821 tgttcatcaa gctgaatgca gggcgtattg gcgggcttgc catctggcat accgggaata
    35881 gggctggaaa ttgaaggggc agtgcaacac gccccgcaac ccggacggca ttccatacgt
    35941 tgttctctta aggttaacca ccggattgat gagcgcgcac agtaccacct tttttgcacc
    36001 agcaaaagtg cgaataccac ttgcccgaaa ggcccgtcgc gagtactttg tcgcgatatt
    36061 tttgacattt tcgactacag gaatttttcg atgccaagag cgaacgaaat taaaaaaggt
    36121 atggtactga attacaacgg caaactgctg ctggttaagg atattgatat tcagtcgccc
    36181 actgcccgcg gtgccgctac gctgtacaaa atgcgttttt ctgatgtccg taccgggctg
    36241 aaagtagaag agcgtttcaa aggtgatgat atcgttgaca ccgtgacgct gacccgccgt
    36301 tacgttgact tctcctatgt cgatggcaac gaatatgtct ttatggataa agaagactat
    36361 accccgtata ccttcaccaa agatcagatt gaagaagagt tgctgtttat gccagagggc
    36421 ggcatgccgg acatgcaggt gctgacctgg gacggtcaac tgctggcgct tgagctgccg
    36481 cagaccgttg atctggaaat cgttgaaacc gcaccaggca tcaaaggggc atctgccagc
    36541 gcccgtaaca aaccggcgac attgagcact ggtctggtga ttcaggtacc agaatactta
    36601 agcccgggcg aaaaaattcg tatccatatc gaagaacgcc gttatatggg gcgtgctgac
    36661 taacttcagc cgcatgcaga aaagggatag ctcaggctgt ccctttttta atttattata
    36721 ccaatcttct attttgcgct tcctgaacac cttacgccct caattttcac tcgttgattg
    36781 atcgccctca cacttcatcg cattaacaat ccagaccaat ttcaattgct gtcatataac
    36841 tttacactgt cattgttaat ttatcgttac taagacgtga ctcctatgaa gacaattgcc
    36901 tccgttacgc tcccgcatca tgtacacgct ccacgctacg atcgccagca gttgcaatca
    36961 cgtatcgttc attttggctt tggcgccttt caccgcgctc atcaggcgtt actgaccgat
    37021 cgtgtgctga atgcccaggg cggcgactgg gggatctgtg aaatcagctt gttcagcggt
    37081 gatcaactga tgagccagct ccgcgcacag aaccatttat ataccgtgct ggagaaaggt
    37141 gcggacggca atcaggtgat aattgtcggt gccgttcacg aatgccttaa tgcaaaactg
    37201 gattccttag cggcaattat tgagaaattt tgcgagccac aggtggcaat tgtttccctg
    37261 acgattaccg aaaaaggcta ttgtattgac ccggccaccg gtgcactcga caccagtaat
    37321 ccgcggatta ttcacgatct acaaacccct gaagaacctc actccgcacc gggtattctc
    37381 gtcgaagcac tgaaacgccg ccgtgagcgc ggccttacac cgtttaccgt gctctcctgc
    37441 gacaatattc ccgacaatgg ccatgtggtg aaaaacgcgg tgctgggaat ggcagaaaaa
    37501 tgttcgccag aactcgccgg gtggataaaa aaacacgtca gttttccggg aaccatggtc
    37561 gaccgcattg ttccggctgc aaccgacgaa tcactggcag aaatcagcca gcatctgggg
    37621 gtaaatgatc cctgcgcgat tagctgcgaa ccgtttatcc agtgggtggt tgaagataac
    37681 ttcatcgctg ggcgtcctgc ctgggaagtc gcaggtgtac aaatggtgaa tgatgtcctg
    37741 ccatgggaag agatgaaact gcgtatgctt aatggcagcc actcttttct cgcttatctg
    37801 ggttacctct caggattcgt ccatatcagt gattgcatgc aggatcgcgt atttcgccat
    37861 gctgccagaa cattaatgct ggatgagcaa gcgccgacac tgcaaattaa agatgtcgat
    37921 ttaacacaat atgcggataa gttaattgca cgttttgcta atccgtcgct gaaacataag
    37981 acctggcaaa tcgcgatgga tggcagccag aaattaccgc aacgtatgct ggcaggtatt
    38041 cgcatacatc tggggcgtga aacggactgg tcgttgctgg cattaggcgt tgctggctgg
    38101 atgcgttacg tcagcggcgt tgatgatgcc ggaaatgcca ttgatgttcg cgatccgctt
    38161 agcgataaaa ttcgcgaact tgttgcgggc agcagcagtg aacaacgcgt aaccgccctg
    38221 ctttccctgc gtgaagtttt cggtgatgat ctgccagata acccgcattt tgtgcaggcc
    38281 atcgaacaag cctggcaaca aatcgtacaa ttcggcgcac atcaggcgct attaaacacc
    38341 ctcaaaattt aacgatttct gcggttaaag cggatgaagc tcaccttcgt ccgctctccc
    38401 cttctctttt ctgccttttt tagccaggat taacgctcag ttaacttacc agagtattgc
    38461 ggagccgttg tgaccaggac caacctcatc accggttttc tcggcagcgg gaaaaccacg
    38521 tcgattcttc atctgttagc ccataaagat cccaacgaaa aatgggcggt actggttaat
    38581 gaatttgggg aagtcggaat tgatggtgct ttgctcgccg atagcggcgc attgctgaaa
    38641 gagatccccg gcggctgcat gtgctgcgtt aatggtttac ccatgcaggt agggttgaat
    38701 accttactgc gtcagggaaa accagaccgc ttgttgatag agccgaccgg gctgggccat
    38761 ccgaaacaga tcctcgatct gttaaccgca ccagtctatg aactgtggat agatctgcgc
    38821 gccaccttgt gcattctcga tccacgcctg ctgctggacg aaaaaagcgc cagcaatgaa
    38881 aacttccgtg accagctggc tgccgcagac atcattgtcg ccaataaatc cgaccgtgcg
    38941 acgcccgaaa gtgagcaagc gctacagcgt tggtggcagc aaaatggtgg cgatcgacaa
    39001 ttaattcaca gtgagcatgg gaaagttgac ggtcatcttc tggatttgcc gcgtcgcaat
    39061 ttagccgagt tgcccgccag cgccgcgcat tctcatcagc atgtcgtgaa aaaagggtta
    39121 gcagcgttaa gcctgccaga gcatcaacgc tggcgtcgca gtctgaacag cgggcaagga
    39181 tatcaggcct gcggctggat attcgacgct gatacggtat tcgacaccat tggcattctg
    39241 gaatgggcgc gacttgcacc ggtggaacgc gtcaaaggcg tgctgcgtat tcccgaaggg
    39301 ctggtacgaa tcaaccgtca gggcgatgac ctgcacattg aaacgcaaaa cgttgcgcca
    39361 ccggacagcc gtattgagct gatttccagc agcgaagctg actggaatgc tttgcagagc
    39421 gcgctgttga agcttcgttt agcgactacc gcgtaaggtt gcctgcgttt ttcagtaaga
    39481 taattagaga aaatatgatt aaaaatttgc cgcaaatagt gttgttgaat attgtcggcc
    39541 tcgcgctgtt tctttcctgg tatattcccg ttaatcatgg attctggttg ccgattgatg
    39601 cggatatttt ttatttcttt aatcagaaac tggtcgaaag taaggccttt ttgtggctgg
    39661 ttgcattgac caacaatcgc gctttcgacg gttgttctct gctggcgatg ggaatgttga
    39721 tgctgagttt ctggctgaaa gaaaacgccc ctggcagacg acgtatcgtg attatgggtc
    39781 tggtcatgct attaactgca gtggtattaa accagctggg tcaggcatta attcctgtaa
    39841 aacgggccag cccaacattg acttttaccg atattaaccg cgtcagcgaa ctgctctctg
    39901 ttcccacgaa agatgcctca cgagatagct ttcccggcga tcacggcatg atgctgctta
    39961 ttttttcggc attcatgtgg cgttatttcg gcaaagttgc aggccttatc gcccttatta
    40021 tttttgtggt ttttgcattt cccagagtaa tgattggcgc acactggttt actgacatca
    40081 ttgtcggttc gatgaccgtg atattgatcg gtttgccctg ggtgttgctg acgccattaa
    40141 gtgatcgatt aatcaccttt tttgacaaat cactaccggg aaaaaacaaa catttccaaa
    40201 acaaataact cacagtaatt aacatcatca ggcttatttt tatagtgagg ataatcctga
    40261 tgatgcgcac cgtgctttca tccatcgcgc gcaaaaatca ttctctaagt aaatgaatgg
    40321 attgcatgcg tttcactcaa tcgtacttta attgaccaat cccgcttatt aactttctgt
    40381 atcacttttt cttataaaaa atcatgtaaa accgctcgcc aagaccgcac caatcgggta
    40441 atctagaact cgttttgcct cggcggtaga ttatcctcac agcatataat tttgtgcgtt
    40501 agtccacaga tttggcctta aggaattgtt tcaacatgcc caggtaatta gtctcgtgtc
    40561 gcttggcatt tttttataac gatatttgtc gttaaggact tcaagggaaa acaaacaaca
    40621 tggtcaaatc tcaaccgatt ttgagatata tcttgcgcgg gatccccgcg attgcagtag
    40681 cggttctgct ttctgcatgt agtgcaaata acaccgcaaa gaatatgcat cctgagacac
    40741 gtgcagtggg tagtgaaaca tcatcactgc aagcttctca ggatgaattt gaaaacctgg
    40801 ttcgtaatgt cgacgtaaaa tcgcgaatta tggatcagta tgctgactgg aaaggcgtac
    40861 gttatcgtct gggcggcagc actaaaaaag gtatcgattg ttctggtttt gtacaacgta
    40921 cattccgtga gcaatttggc ttagaacttc cgcgttcgac ttacgaacag caggaaatgg
    40981 gtaaatctgt ttcccgcagt aatttgcgta cgggtgattt agttctgttc cgtgccggtt
    41041 caacgggacg ccatgtcggt atttatatcg gcaacaatca gtttgtccat gcttccacca
    41101 gcagtggtgt tattatttcc agcatgaatg aaccgtactg gaagaagcgt tacaacgaag
    41161 cacgccgggt tctcagccgc agctaataaa ccgtttggat gcaatccctt ggctatcctg
    41221 acgagttaac tgaaagcact gcttaggcag tgcttttttg ttttcattca tcagagaaaa
    41281 tgatgtttcc gcgtcttgat ccaggctata gtccggtcat tgttatcttt taaatgttgt
    41341 cgtaatttca ggaaattaac ggaatcatgt tcatacgcgc tcccaattct ggacgtaagc
    41401 tcctgcttac ctgcattgta gcaggcgtga tgattgcgat actggttagc tgccttcagt
    41461 ttttagtggc ctggcataag cacgaagtca aatacgacac actgattatc gacgtacaaa
    41521 agtatctcga tacctatttt gccgacctga aatccactac tgaccggctt cagccgctga
    41581 ccttagatac ctgccagcag gctaaccccg aactgaccgc tcgcgcggcg tttagcatga
    41641 atgtccgtac gtttgtgctg gtgaaagata aaaaaacatt ctgttcatct gcgactggtg
    41701 agatggacat tccactcaat gaattgattc cggcgctcaa cattaataaa aacgtcgata
    41761 tggcgatctt acccggcacg ccgatggtgc cgaacaaacc cgcaatcgtc atctggtatc
    41821 gcaacccttt gctgaaaaat agcggtgtgt ttgccgctct gaatctcaac ctgacgcctt
    41881 ctctctttta tagttcacgg caggaagatt acgatggcgt cgccctcatt attggcaata
    41941 ctgcgctatc taccttttct tcacgtttga tgaacgttaa tgaattaacc gacatgccgg
    42001 tccgtgaaac taaaattgcg ggcattcctc tgaccgttcg gctttatgcg gatgactgga
    42061 catggaacga tgtgtggtac gcatttttac tgggcggcat gagtggaact gtcgttggcc
    42121 tgctctgcta ttacctgatg agcgtacgta tgcgccccgg cagagaaatc atgaccgcca
    42181 tcaagcgcga acaattttac gtggcgtatc aaccggtggt ggatacacaa gctttgcgag
    42241 taacgggcct ggaagtactg ctacgctggc ggcatcctgt cgcgggagaa attcccccgg
    42301 atgccttcat taactttgcc gaatcgcaaa agatgattgt gccgctgact cagcacctgt
    42361 ttgagttaat tgcccgcgat gccgcagaat tagaaaaagt gctgccagta ggcgtcaaat
    42421 ttggtattaa cattgcgccg gaccatctgc acagcgaaag ctttaaagca gatatccaga
    42481 aactgctcac ttccctgccc gcacaccatt tccagattgt gctggaaatt accgagcgcg
    42541 atatgctgaa agagcaagaa gccacacaac tcttcgcctg gctgcactcg gtcggcgtag
    42601 aaattgctat tgatgacttc ggcaccgggc acagcgcgct tatctatctt gagcgtttta
    42661 cgctcgatta tctgaaaatt gaccgtggat ttatcaacgc catcggtacg gaaacgatca
    42721 cttcacccgt acttgacgcg gtgctgacgc tggcgaaacg cctcaatatg ctgacggttg
    42781 ctgaaggggt cgaaacgccg gaacaggcgc gatggctaag cgaacgcggc gttaatttca
    42841 tgcaaggcta ctggattagt cgccctttac cgctggacga ttttgtccgc tggctgaaga
    42901 aaccgtatac gccgcagtgg taaggtgtgc ttacgtccct tattattcat agtgaaagca
    42961 tgccggattg cggctaatga tgagtaaaag gaaatccatt gcagatgatt gtgcgcatac
    43021 tgctgctgtt tatcgctctg ttcacctttg gtgtgcaggc gcaggctatc aaggaaagct
    43081 atgcctttgc cgtgctgggc gaaccccggt acgcgtttaa tttcaaccat tttgattatg
    43141 tgaaccccgc cgcgccaaaa ggtgggcaga taacgttgtc agccctcggc accttcgata
    43201 atttcaaccg ctatgcactg cgcggcaacc cgggcgcacg caccgagcag ctgtacgaca
    43261 cgctatttac gacttccgat gacgaaccag gcagttatta cccgctgatt gctgaaagcg
    43321 cacgctatgc tgacgattat tcctgggtgg aggtcgctat taatccgcgc gcccgttttc
    43381 atgatggttc gcccattact gcccgcgatg tagagtttac ttttcaaaaa tttatgaccg
    43441 aaggcgtgcc gcaatttcgt ctggtctaca aaggcaccac cgtcaaagcc attgcaccgt
    43501 taaccgtgcg cattgagtta gctaaaccca gcaaagaaga tatgctgagt ctgttttcgc
    43561 tgccgatatt tccagaaaag tactggaagg atcacaaact tagcgacccg ctcgccacgc
    43621 ctccgcttgc cagtggtccg taccgcgtta cgtcctggaa aatggggcaa aatattgtct
    43681 attcccgcgt gaaagattac tgggcagcaa acttaccggt aaaccgtgga cgctggaatt
    43741 tcgacaccat tcgctacgat tattacctcg atgataatgt cgcctttgaa gcgtttaaag
    43801 caggtgcctt tgatttgcgt atggaaaacg acgctaaaaa ctgggccacg cgttataccg
    43861 gtaaaaattt cgataaaaaa tacatcatca aagatgagca aaagaacgaa tcagcccagg
    43921 atacgcgctg gctggcgttt aatatccaac gtccggtatt cagcgatcgc cgggtccggg
    43981 aagcaatcac tctcgccttt gactttgaat ggatgaacaa ggcgttgttt tacaacgcct
    44041 ggagtcgcac gaacagttat tttcagaata ccgaatacgc ggccagaaat taccccgacg
    44101 ccgcggagct ggtgcttctg gcaccaatga aaaaagatct accgccagaa gtcttcacac
    44161 aaatctacca gccgccggta tctaaaggcg atggctacga tcgtgacaac ctgttaaaag
    44221 ccgacaaact tctcaacgaa acgggctggg tgctgaaggg tcagcaacgc gttaatgcca
    44281 caacgggtca gccgctcagc tttgaattat tgcttcccgc aagcagcaat agtcagtggg
    44341 tattgccgtt ccagcacagc ctgcaacggc tgggtatcaa tatggacatt cgcaaggtgg
    44401 ataactcgca aatcactaac cgcatgcgca gtcgcgacta tgacatgatg ccgcgcctat
    44461 ggcgggcgat gccgtggccc agttccgatt tacagatttc ctggtcatcg gaatatatca
    44521 attccactta taatgccccc ggcgtgcaaa gcccggttat cgactcgctg atcaaccaaa
    44581 ttattgccgc gcagggaaat aaagaaaaat tactgccgtt ggggcgagca ctggatcgcg
    44641 tattaacgtg gaattattac atgctgccaa tgtggtacat ggcggaagac cgtctcgcct
    44701 ggtgggataa attctcccaa cccgctgtac gccctgttta cagcctgggt atcgatacct
    44761 ggtggtatga cgttaataaa gcggccaaac tgccgtcagc cagacaacag ggagagtaga
    44821 tgggcgctta cctgattcgc cgtctgttgc tggtgatccc aacgttatgg gctattatca
    44881 ctatcaactt tttcatcgtg caaattgcgc ctggcggtcc ggtcgatcag gccatcgccg
    44941 ccattgagtt tggtaatgcc ggagtattac ccggcgcagg cggtgaaggt gttcgtgcca
    45001 gccatgcgca aacgggtgtc ggcaatatca gcgacagtaa ttaccgtggc ggacgcggat
    45061 tagacccaga agtgatcgct gagatcactc atcgctacgg tttcgataag ccgatccacg
    45121 aacgttactt caaaatgctc tgggactaca tccgctttga ttttggtgat agcctgtttc
    45181 gcagcgcctc ggtgctgacg ctgattaaag acagtctgcc ggtttccatc accctcggat
    45241 tgtggagcac gctgattatc tatctggtgt cgattccgtt aggcattcgc aaagctgttt
    45301 ataatgggag tcgctttgac gtctggagta gcgcatttat catcatcggc tacgccattc
    45361 cggccttttt gtttgccatc ctgctgattg tcttcttcgc gggcggcagc tatttcgacc
    45421 tgttccctct acgcggcctg gtttccgcta actttgattc gctgccgtgg tatcagaaaa
    45481 tcaccgatta tctgtggcat atcacgctac cggtgctggc gacagtgatt ggtggctttg
    45541 cggcgctgac catgctgacc aaaaactcat tccttgatga agtgcgcaag caatacgtgg
    45601 tgaccgcgcg tgcgaaaggg gtaagtgaga aaaacattct ctggaaacat gtgttccgca
    45661 acgccatgct gctggtgatt gccggttttc cggcgacgtt tatcagcatg ttttttaccg
    45721 gctcgctgct gattgaggtg atgttttcac tcaatggtct tggcttactg ggctacgaag
    45781 cgaccgtctc gcgcgattat cctgtaatgt ttggtacctt gtatattttc accctgattg
    45841 gcctgctgct gaatattgtc agtgatatca gctatacgct ggttgatccg cgtatagatt
    45901 ttgagggacg ttaatgtcgc gactcagccc cgtcaatcag gcccgttggg cgcgttttcg
    45961 tcataaccgt cgcggctact ggtcgttatg gattttcctc gttttgtttg gtttgagttt
    46021 gtgttctgaa cttatcgcta acgataaacc gttgctggtg cgttatgacg gcagttggta
    46081 tttcccgttg ttgaaaaact acagcgaaag cgattttggc ggcccgctgg caagtcaggc
    46141 tgattatcag gacccgtggc tgaaacaacg gctggaaaat aacggctggg tactgtgggc
    46201 accgattcgc tttggtgcta ccagtatcaa ctttgctacc gataagccct tcccttctcc
    46261 accctcccgg caaaactggc tgggaacgga tgccaacggc ggcgatgtgc tggcacgcat
    46321 tctctatggc acgcggatct cggttctgtt tggcctgatg ctgactctct gttccagcgt
    46381 gatgggcgtg ctggcggggg cgctacaagg ctattacggc ggtaaagtcg atctctgggg
    46441 acaacgcttt attgaagtat ggtcggggat gccgacgctg tttttgatta ttttactttc
    46501 cagcgtcgta cagcctaact tctggtggct gctggcaatt actgtcttgt ttggctggat
    46561 gagtctggtc ggcgtggtgc gggcggagtt tttacgtact cgtaatttcg actacattcg
    46621 tgcggcacag gcgcttggcg tcagcgatcg cagtatcatc ctgcgtcata tgttgcctaa
    46681 tgccatggtc gcgaccctca cctttttacc gtttatttta tgtagttcga taaccaccct
    46741 gacctcgctc gatttcctcg gcttcggtct accgctcggt tcaccgtcac tcggtgaact
    46801 gctgttacaa gggaaaaata accttcaggc cccgtggctt gggatcaccg ccttcttgtc
    46861 ggtggcgata ttattgtctt tgctgatctt tattggtgaa gccgtccgcg acgcatttga
    46921 acctaataag gcggtgtagc atgacgcaaa ctctgttagc gattgaaaat ttgtcggtgg
    46981 gttttcgcca tcagcaaacc gtacgtacag tagtcaatga tgtttcacta cagattgagg
    47041 ctggcgaaac gctggcgctg gtgggtgagt caggttcagg caaaagcgtt accgcgctgt
    47101 caattttacg cctgctccct tccccgccgg ttgaatatct ctccggcgat attcgttttc
    47161 atggcgaatc gctgcttcat gccagcgatc aaacgttgcg cggtgtacgc ggtaataaga
    47221 tcgccatgat ttttcaggaa ccgatggtgt cgttaaatcc attgcatacc ctggaaaaac
    47281 agctttatga agtgctttca ctccaccgcg ggatgcgtcg ggaagcggct cgtggcgaaa
    47341 ttcttaactg ccttgatcgc gtcggtatcc gccaggcggc aaaacggctg acagattatc
    47401 cgcatcagct ctccggcggc gaacgccagc gggtgatgat tgcgatggcg ctgttaacgc
    47461 gaccggaatt attaattgcc gatgaaccga ccaccgcact ggacgtctct gtccaggcgc
    47521 agattttaca gctgttgcgc gaactgcaag gcgagctgaa tatgggcatg ctgtttatta
    47581 ctcataacct cagcattgtc agaaaactgg cccaccgcgt ggcggtaatg caaaacggtc
    47641 gctgtgtcga gcaaaataac gccgctacgc tatttgcctc acccactcat ccttacacac
    47701 aaaagctact caacagtgaa ccgtcaggcg atccggtacc gttgccagaa cctgcctcaa
    47761 cgttgctgga tgttgaacag cttcaggttg ccttccccat tcgcaaaggg attttgaagc
    47821 gcattgtgga tcataatgtg gtggtgaaaa acatcagttt tacgctacgg gcgggtgaaa
    47881 cactgggttt agtgggcgag tctggttccg ggaaaagcac gacgggactg gcgctgctgc
    47941 gactgattac ctctcagggc agcatcgtct ttgacggtca gccactgcaa gatttaaatc
    48001 gccgccagct gttacctact cgtcatcgta ttcaggtggt atttcaggat ccaaactcct
    48061 cgctcaaccc acgactcaac gttttgcaga ttattgagga aggcttacgg gttcaccagc
    48121 cgacgctttc tgccgcacaa cgcgaacaac aagtgatagc cgtgatgcat gaagtgggat
    48181 tagatcctga aacacgccac cgttatccgg cggagttctc tggtggtcag cgacaacgta
    48241 ttgcgattgc cagggcatta attcttaagc cctcactgat catacttgat gaaccgacat
    48301 catcactcga caaaacggta caggcgcaaa tattgacgct attgaaatca ttgcaacaaa
    48361 agcatcaact ggcctatttg tttatcagcc acgatttgca cgttgtccgc gcgttatgtc
    48421 atcaggttat cgtactgcga caaggggaag tagtggaaca aggaccgtgc gcgcgcgtgt
    48481 ttgccgcacc gcagcaggag tatacgcgtc agctactggc gttgagctga cgcttaaaaa
    48541 ggattgtagt ttgaaaaggg ttcggcaatt gccacaccaa aatttttcaa tcggcatgtt
    48601 gcagcaaact cgtcctggcg atttacaaac aggcacggct caccttcaca ctccagcacc
    48661 gaacatggaa cttcgatgtc gcttaacgcc tggcttagtt tatacatcac cgaccatgca
    48721 ttatcaccat ccgggctaag caatttaagc gccaccaggc tgttgtcacc gcacggtcca
    48781 ttttgcggaa tcggttcaac cttcgaacct gcgaaaccag cagaccagcg aaagttctgc
    48841 ggtagacgat ggacgattga gagttgtaat gaagtcactt attttccccg gaagcacatt
    48901 tacttcacaa tttatttaca tcaattttaa cacatcatca acaatccgtc tttatacaga
    48961 tcgtaaagat tgatgcctcg cctgcatggc ctctatggct ctgatttaag taatatctgc
    49021 gggcgtggtc acgtttgcgg ggctatatgt acccgtttct gcgatctaac tcaacctttt
    49081 taactacaat gatgtgactt tttacataaa ttgattttac ataaaataaa catatatcgg
    49141 gggaatgata gatttgtggt tgacatgcat caacaaagga agccttttag cttcctcgtt
    49201 gtgcaataga tcaccgtttt ttcggtcgac tggcgtacag acagaagaga atggagctgg
    49261 ttgcgcagaa tgcaattgac caaatcatcg gccatgcaga gttaaaggtc gcaagagaaa
    49321 gcaatgcgcc aacaattgcc cctatgccaa aacggaaggt tcctgccagc gaagatgccg
    49381 ttcccgccat atggggaaac tcatcaagaa tgaccgccat cgcattggat gacaccatag
    49441 acacgcagcc cacaaacgcc gcaacgccaa ccaccagcga ccagaatccc agccccagca
    49501 gcgcactgat gaccatccac gctgccataa taaattgtat ccacaacccc gagcggaaca
    49561 tatttaacgc gccaatgcgg cggacgaagc ggctgttaaa gatggtcatc acgaacagaa
    49621 aaacaatgtt tagcgcaaag taataaccaa agttttccgg tgcaacgtgg ttgatttcaa
    49681 tgtagacaaa cggtccggcg ctcaggaacg agaacattcc ggcaaagctg aaaccgctgg
    49741 ccagcatata actcaggaca cgcttatgac ggaacagcgc cgcaaagtta ccaatagtgg
    49801 tacgaatgtg aaaaggctgg cgaggatcca gaagatgtaa tgccagctca gccacaccag
    49861 cacccagccg ccaactatcg gtgccatcag cggtgcaatg gtcgtcacca gcatgacaaa
    49921 cgacatcatc cgcgagaact cttctttcgg gtaaatatca cgcatcaaag cgttgataac
    49981 cacgctggcc gcagccgcag ccagcccgtg gaagaaacgc atcacaatca gctgatcgat
    50041 ggtttgtgcc aacgcacacg ccaccgcggc ggcggcaaac accagcgtac cgccaagaac
    50101 caccggctta cgcccgaagc tgtctgccat cggcccgtag attaactgcc ccaacgcaaa
    50161 gcccagaata taagtactca gggtcatctg cgtactgccc gccggtacgc caaactgcgc
    50221 tgaaattacc ggtagcgcgg gcagatacat atcaatcgac agcggcatca acatggccag
    50281 caggccaagg ataaaaacaa tagcaaacga cgaatgctgt cgggtggtca caacgggctc
    50341 ctgaaagtca ttgaaaagtt agacgacgct ggcaatttct tcttcagtta acggacgata
    50401 ttcaccgggg gctaaatcag catccagcgt aatgccgcca atacgttcac gatgcagctc
    50461 aaccacatgg ttacccacgg cggcgaacat gcgtttcacc tgatgataac gcccttcgct
    50521 gatggtcaga cgaacctgcg ttggggtaat cacttccagc accgccggct tagtgagatc
    50581 tttttcgtta tgcagctgca cgcctttagc aaattgttct gccgtatcgt cagctacggg
    50641 tgataccagc gtcaccagat aggtcttctc gcaatgatgg cgcggagaag taatgcggtg
    50701 cgaccactga ccatcatctg tcattagtac cagaccggtg gtatcgatat ccagccgccc
    50761 tgcggcgtgc agtttccacg ctaccggttc atctagaaaa tagagcaccg ttgggtgatc
    50821 agggtcgtcc gtggagcaaa cataaccctg aggcttattg agcatgaagt aacgtggacc
    50881 gtgttgctgc gccagcgggt tgccatcgta agcgacatca tgttcaggaa gcagtttgaa
    50941 cgctgcatta cggacgattt cgccatcgac ggtgacacga ttgccgcgga tttcacgccc
    51001 ggcaatagca cggctaacgc cgagttgctg tgcgataaat ttatcaagtc gcatgtgtgt
    51061 gattttgcct gtaaaaaacg gaggtcgggt aatgacccga atatctgaac tgttgtctgc
    51121 ccagtatagc ggtctaataa catccctcaa gggaaaaaga tccgtggcat actattagca
    51181 gaataatcta cctacgcgag accatgattt ttacacttcg cccatatcag caagaagccg
    51241 tggatgccac gctcaaccat tttcgtcgtc ataaaacccc tgccgttatc gtgctgccca
    51301 ccggtgcagg taaaagcctg gtgatagcgg aactggcacg gctggctcgt ggtcgcgtac
    51361 tggtgctggc acacgttaaa gaactggtgg cgcaaaacca tgcaaagtat caggcgctgg
    51421 ggctggaagc cgatattttt gccgccgggc tgaagcgtaa agagagccac ggtaaagtgg
    51481 tatttggcag tgtgcagtcg gtcgcccgta atattgatgc ctttcagggt gaattttcgc
    51541 tgttgattgt cgatgaatgt caccgtattg gtgacgatga agagagccag tatcagcaaa
    51601 tcctcactca cctgacaaaa gtgaatcccc acttacgcct gctggggctg actgccacgc
    51661 cttttcgatt gggcaaaggt tggatctacc agttccatta tcacggcatg gtacgcagcg
    51721 atgagaaagc ccttttccgt gactgcattt atgagctgcc cctgcgttat atgattaaac
    51781 acagctatct gacgccgcca gaacgactgg atatgccagt agtgcaatat gatttcagcc
    51841 gcttgcaggc acagagcaac gggctgttca gcgaagccga tctcaaccgt gagctgaaaa
    51901 aacagcaacg tattaccccg cacatcatca gccagattat ggagttcgcc gaaaagcgta
    51961 aaggagtgat gattttcgcc gcgacggtcg aacacgcaaa agagattgtg ggattactgc
    52021 ctgccgaaga tgcagcactg attactggcg acacccccgg cgctgagcgc gatgtgttaa
    52081 ttgaaaattt taaagcccag cgttttcgct atctggtcaa cgtcgcggta ctgaccaccg
    52141 gatttgacgc cccgcacgtc gatcttatcg ccattctgcg ccctaccgaa tcagtgagtc
    52201 tttaccaaca aattgtcggg cgcggtctgc gtctcgctcc gggcaagact gattgtttaa
    52261 ttcttgatta tgcgggtaat cctcacgatc tctacgcgcc ggaagttggt acaccgaaag
    52321 gcaaaagtga caacgttccg gtacaggttt tctgccctgc ctgcggtttt gccaacacct
    52381 tttgggggaa aacgaccgcc gacgggacat tgattgaaca ctttggtcgc cgctgtcagg
    52441 gatggtttga agatgacgac ggtcatcgcg aacagtgtga cttccgtttc cgttttaaaa
    52501 attgcccgca atgtaacgca gaaaatgata ttgccgctcg ccgctgccgc gaatgtgaca
    52561 cggtactggt tgatccggat gatatgttaa aagcggcgct acgactgaaa gatgcgctgg
    52621 tattacgctg tagcggcatg tctttgcagc atgggcacga cgagaaaggc gaatggttga
    52681 aaatcaccta ttacgatgaa gacggtgcgg atgtgagtga gcgtttccgt ctgcaaacac
    52741 ctgcccagcg taccgccttc gagcagcttt ttatccgccc gcatacgcgc acaccgggca
    52801 tcccgctgcg ctggatcacc gccgccgata tcctcgccca gcaagcctta ttgcgacccc
    52861 cggattttgt cgtcgcccgc atgaaaggcc agtactggca ggtgcgtgaa aaagtgttcg
    52921 attacgaagg tcgttttcgt cgggcgcacg aattacgcgg ttaatggtag ttttgattga
    52981 tgtataaggc gattgagtat agaatctcac ccgcttttgc atacgcaaag cagatcactt
    53041 acctgttgct gggtcgcctg tagcaggatt aatttaagag agaaagaaat gtttactatc
    53101 aacgcagaag tacgtaaaga gcagggtaag ggtgcgagcc gccgcctgcg tgccgctaac
    53161 aagttcccgg caatcatcta cggtggcaaa gaagcgccgc tggctatcga gctggatcac
    53221 gacaaagtca tgaacatgca agctaaagct gaattctaca gcgaagttct gaccatcgtt
    53281 gttgacggta aagaaatcaa agttaaagct caggacgtac agcgtcaccc gtacaaaccg
    53341 aagctgcagc acatcgactt cgttcgcgct taattgctga ataagttgta aaaaaccccg
    53401 ctcccgcggg gttttttgta tctgcagatt atgcctgatt acggtattgc tattttttgc
    53461 aggccagata aggcgttcac gccgcatctg gcattcagaa cacaaaaccg attaattgcc
    53521 gccagacgtc cggcgctgca attggtcgcg caaattcggc ggcgtgcctt taatggtcag
    53581 ggtatcggtc gccggctccc agaagatgcg ttcacctaac agcatcgcat caaagttaat
    53641 agtcaagcca ccgccgctac cggcaaactt cgtcaactga cgcagtgtgc tccggtctgc
    53701 cgggaagctc tcttccagtt cgtagccttt ttcagccgca aactccgtga agctcacttc
    53761 gcttacgcct gccagctctt tcgacagcga ttccagttcg atctcttcgc ctgcctgcag
    53821 ctgctcatta cagtagctat aaacctgctg gcgcacgttc tgtcgctctg ctttatccag
    53881 ttgtgcttcg gcagtgaaat catcaaccgc ttgcaacagc ccacggtttt gtgctttcgt
    53941 gttcaatcct tcgctggccc cgaggaaatc cataaagaaa tccgccactt tgcgccctac
    54001 tcgccctttc aggaaagtga gatagcgggt ggactctgga ttggtttccc attcggttaa
    54061 atcgatacgc gcaacaatat ccgcatgatt gatatcaaga taatgggttg ggttgatgtc
    54121 gagattttcg ttaacacgca tactgctcag gttgctcaga actgccacca gcagatactc
    54181 caccgccaga taacggtagt ggcaaaacag cacgaatcca ccgtcagcaa aaggatattt
    54241 cgctaattcg tcacgcaggc gaccagttgc cgcgcggcta aatgccagaa agtcctcttc
    54301 gccctgacgc tgtaagcgca gcgtctgcgc cagttcactc tcttcgctaa acaagccgta
    54361 agctttattt ttggcgctat agacccgatg cagttctgcc accatctcaa cgacggtttc
    54421 tgtcggttcc agcaatgaat cgcgcaacac cagctcaagg ttctgctcat cacgcttgat
    54481 aagctggtgc agagcaatct ggttgatatc cagactcatg ataaactctc ctttaagacc
    54541 gggcggtatt caaccaccgc cggttaagcg acgcaataaa agatggcggt cattttacag
    54601 aaggcagcgt aacgagaatg atttaacgca taaaaaagca gaaaaaaaac cgttgctacg
    54661 gtaatatgtt gccctttcat caactaactg atttcgattt atgccacaaa tttcccgcta
    54721 cagtgatgaa caggttgaac aattgctcgc agagctgctc aacgtactgg aaaaacataa
    54781 ggctccgacc gacctttccc tgatggtgtt aggcaacatg gccactaacc ttatcaacac
    54841 cagcattgcc ccggcccaac gccaggcaat tgccaactct tttgcccgcg ccttacagtc
    54901 ctctatcaac gaagacaaag cgcactaagg gaaacagata acaggttatg gtaactcatc
    54961 gtcagcgcta ccgtgaaaaa gtctcccaga tggtcagttg ggggcactgg tttgcactgt
    55021 tcaatattct gctttcgctc gtcattggca gccgttacct gtttatcgcc gactggccga
    55081 caacgcttgc tggtcgcatt tattcctacg taagcattat cggccatttc agcttcctgg
    55141 tgttcgccac ctacttgctg atcctcttcc cgctgacctt tatcgtcggc tcccagaggc
    55201 tgatgaggtt tttgtccgtc attctggcaa cggcgggaat gacgctatta ctgatcgata
    55261 gcgaagtctt tactcgtttc catctccatc ttaatcccat cgtctggcag ttggttatca
    55321 acccagacga aaatgagatg gcgcgcgact ggcagctgat gttcatcagc gtgccggtta
    55381 ttttattgct tgaactggtg tttgcgacgt ggagctggca aaagctgcgc agcctgacgc
    55441 gtcgtcgacg cttcgcgcgc ccgctggccg cattcttatt tatcgccttt atcgcctcgc
    55501 atgtggtgta tatctgggcc gatgccaact tctatcgccc gatcaccatg cagcgcgcta
    55561 acctgccgct ttcgtacccg atgacggcgc gacgttttct tgagaagcat ggtctgcttg
    55621 atgcgcagga gtatcaacgc cgtcttattg agcaaggtaa tccagacgcc gtttccgttc
    55681 agtatccgtt aagcgaactg cgctatcgcg atatgggcac cgggcagaat gtgctgttga
    55741 ttactgtcga tggcctgaac tactcacgct tcgagaagca gatgcctgcg ctggcaggtt
    55801 ttgctgagca aaatatttcg ttcacgcgcc atatgagctc cggcaacact acagacaacg
    55861 gcatctttgg cctgttctat ggtatctcgc cgagctatat ggacggcatt ctgtcgaccc
    55921 gtacgcctgc ggcattaatt actgcgctta atcagcaagg ctatcagctg gggttattct
    55981 catcagatgg ctttaccagc ccgctgtatc gccaggcatt gttgtcagat ttctcgatgc
    56041 cgagcgtacg cacccaatcc gacgagcaga ccgccacgca gtggatcaac tggctgggcc
    56101 actacgcaca agaagataac cgctggttct cgtgggtttc tttcaatggc actaacattg
    56161 acgacagcaa tcagcaggca tttgcacgga aatatagccg ggcggcaggc aatgtcgatg
    56221 accagatcaa ccgcgtgctc aatgcactgc gtgattctgg caaactggac aatacggttg
    56281 tgattatcac tgccggtcgg ggtattccgc tgagcgaaga cgaagaaacc tttgactggt
    56341 cccacggtca tctgcaggtg ccattagtga ttcactggcc aggcacgccg gcgcagcgta
    56401 ttaatgcgct gactgatcat accgatctga tgacgacgct gatgcaacgc ctgctacatg
    56461 tcagcacacc tgccagcgaa tattcgcaag gtcaggattt gttcaaccct caacgccgtc
    56521 attactgggt tactgcagcg gataacgata cgctggcaat taccaccccg aaaaagacgc
    56581 tggtgctgaa caataacggt aaataccgca cttacaactt acgtggtgaa agagtgaaag
    56641 atgaaaaacc acagttaagt ttgttattgc aagtgctgac agacgagaag cgttttatcg
    56701 ctaactgatt aattataaat cagttagcga aatatcttac ttgcaatcgg tgtggaaaac
    56761 ggtagtatta gcagccacga gtcggcacgt agcgcagcct ggtagcgcac cgtcatgggg
    56821 tgtcgggggt cggaggttca aatcctctcg tgccgaccaa aaatcccaag aaaaaaccaa
    56881 cctttacggt tggttttttt atatctgcaa ttaatttgat aaacagaccg tgacacatca
    56941 cagcctgttt attttctgtt atcagaacgt ccagaccact cccgcctgag tattccacgg
    57001 cgattctacc cctgcgccat tgccatagct gactgacaaa tgaccgctta acgtcggggt
    57061 aaacgatgac cttatcccag cctgataaac gccacgggtt ccagccacat cattattaaa
    57121 tttgccatcg tcattaactt tcacatggtt agaatcggcg tattcctgac gcacggcggc
    57181 tttcagccag ggttccaacg tcgtaccgtt ttgcaggtcc atgtgatagc ttaccgccgt
    57241 tcccgcttca gcgcgtaata tccgggtatt tcccacatcc gcgcgcatgc cgtttgataa
    57301 cgtgtagtcc tgaccatctg tggtaaagcc ggtaaaggcg agatagggtc taacactcca
    57361 caatccgtca acccaacgga acccgctctc aacatgagca cccgcgccgt tactattgta
    57421 atcgccaaac gctgttgccc cattactcat cttgccatgg atggtgttgg caaaacggtc
    57481 aactttcacc accccatcaa cataggcacc gttctgatgc tcccaaccgg cataagcccc
    57541 cagggtgtag ctatcaatat tgcctttgcc gccgcgatca aaaccaatat cagaatgaga
    57601 gtaatcaaag aacaagccgc gaattgtgct gctttcttca cgggagaaac ggctatcgat
    57661 accgagcgtc aggcccgtca atgtttgctc aaagccagct cccgcatcag tggtcacgtt
    57721 gttgcgggtg ttaattgccg aactccacat cgccgtatcg taattaacgc cttttacgct
    57781 accaagacgc tcacgcacgg tgtccagttc tgcatcaaat accagcggtt gtgctgctgc
    57841 catgttcagc acatcagtgg ttgaaggggt aatttgcgcg cgattctctg cgaggctcca
    57901 gctatggttg ccattatcca gcaaggtata ttcatacgta ccgatatcaa caacgcctcc
    57961 ggcattgccc aacgtgaatg cagcatcacc gccgcccgtc gttaccagtg taaggctatc
    58021 tcctgctgcc gggctggccc ccgtgtccgt cacgaatatt ttgaaatcac ctgttgcctg
    58081 accgatgacg ttgagctgat cgctctgatg attagccata tcggtacgca tataaaattg
    58141 ccgtttcccg acagggtatt ggtggtcaac gtaatgaaat tttccgcact tgccgtcaca
    58201 gatgaacgag tcactggctc agccatcata atcacatttg cgccggacat cgcaaggttg
    58261 gctaagttga ggcgagtgtt ttggttcgtc gtagtgatat cggcaatgat ggtccatgcg
    58321 ctattttcga gcgaaacgtc cgcttggctg gtatccacgg caccatgcgt tctaaaagaa
    58381 gcgccctcag aaaccactac cttacccacc aacatagcgg gtgcataatt tattttcggc
    58441 ttcatgacct caagaatgcc atcattccct ctgactgaca cgttaaccat tgtgccagcc
    58501 cagacattag cgcggccatt gttgataacc atattttcag agatggcttt agaggaatac
    58561 gtgatacttc cattctgatc tgatcgaccg agatcatata cagcatttgc ctgcactttc
    58621 gtaccagtat cctttcccag cgattgcata gtggcatgct catcaaggat agtgtcaatg
    58681 gcctgagtgt cctccataac aatgagcccg gagccatcat ccagttcata attttttgac
    58741 acaccatcct ttatactaaa ttggccttta ctgtttgtac cactcacttc cagcgcattc
    58801 gttgagacaa ttaatttccc gcctgctttt ttatcaacgc cagttgcagt cccgccatcc
    58861 ataacctcca gtaatcctcc cttttcgata gcggcaaaga cggcaatccc atctttttca
    58921 acaattaagc gcccgccttt aatcgtggga tcattcgccg cg
//
LOCUS       DADMKF010000007        57431 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.007, whole genome shotgun sequence.
ACCESSION   DADMKF010000007 DADMKF010000000
VERSION     DADMKF010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 57431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57431
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.007"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            146..1489
                     /gene="argG"
                     /locus_tag="JNC24_000392"
     CDS             146..1489
                     /gene="argG"
                     /locus_tag="JNC24_000392"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HAY9157238.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGVPQVENLENKGQ"
     gene            complement(1497..3104)
                     /locus_tag="JNC24_000393"
     CDS             complement(1497..3104)
                     /locus_tag="JNC24_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011378876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HAY9157239.1"
                     /translation="MLEDFKSHWLLYLCVIVFGITNLVASSGAHMVQRLLFFVLTILV
                     VKRISSLPLRLLVAAPFVLLTAADMSISLYSWCTFGTTFNDGFAISVLQSDPDEVVKM
                     LGMYIPYLCAFAFLSLLFLAVIIKYDVSLPTKKVTGILLLIVISGSLFSACQFAYKDA
                     KNKKAFSPYILASRFATYTPFFNLNYFALAAKEHQRLLSIANTVPYFQLSVRDTGIDT
                     YVLIVGESVRVDNMSLYGYTRSTTPQVEAQRKQIKLFNQSISGAPYTALSVPLSLTAD
                     SVLSHDIHNYPDNIINMANQAGFQTFWLSSQSAFRQNGTAVTSIAMRAMETVYVRGFD
                     ELLLPHLSQALQQNTQQKKLIVLHLNGSHEPACSAYPQSSAVFQPQDDQDACYDNSIH
                     YTDSLLGQVFELLKDRRASVMYFADHGLERDPTKKNVYFHGGREASQQAYHVPMFIWY
                     SPVLGDGVDRTTENNIFSTAYNNYLINAWMGVTKPEQPKTLEEVIAHYKGDSRVVDAN
                     HDVFDYVMLRKEFTEDKQGNPTPEGQG"
     gene            complement(3582..3668)
                     /locus_tag="JNC24_000394"
     tRNA            complement(3582..3668)
                     /locus_tag="JNC24_000394"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3632..3634),aa:Leu,seq:gag)
     gene            complement(3683..4015)
                     /gene="secG"
                     /locus_tag="JNC24_000395"
     CDS             complement(3683..4015)
                     /gene="secG"
                     /locus_tag="JNC24_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HAY9157240.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNKGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(4243..5580)
                     /gene="glmM"
                     /locus_tag="JNC24_000396"
     CDS             complement(4243..5580)
                     /gene="glmM"
                     /locus_tag="JNC24_000396"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003905541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HAY9157241.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGSRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(5573..6421)
                     /gene="folP"
                     /locus_tag="JNC24_000397"
     CDS             complement(5573..6421)
                     /gene="folP"
                     /locus_tag="JNC24_000397"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HAY9157242.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(6511..8445)
                     /gene="ftsH"
                     /locus_tag="JNC24_000398"
     CDS             complement(6511..8445)
                     /gene="ftsH"
                     /locus_tag="JNC24_000398"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HAY9157243.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(8545..9174)
                     /gene="rlmE"
                     /locus_tag="JNC24_000399"
     CDS             complement(8545..9174)
                     /gene="rlmE"
                     /locus_tag="JNC24_000399"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HAY9157244.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            9300..9593
                     /gene="yhbY"
                     /locus_tag="JNC24_000400"
     CDS             9300..9593
                     /gene="yhbY"
                     /locus_tag="JNC24_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HAY9157245.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(9749..10225)
                     /gene="greA"
                     /locus_tag="JNC24_000401"
     CDS             complement(9749..10225)
                     /gene="greA"
                     /locus_tag="JNC24_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HAY9157246.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            10473..11906
                     /gene="dacB"
                     /locus_tag="JNC24_000402"
     CDS             10473..11906
                     /gene="dacB"
                     /locus_tag="JNC24_000402"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HAY9157247.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYITQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAQKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPGDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     EPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(12092..13264)
                     /gene="cgtA"
                     /locus_tag="JNC24_000403"
     CDS             complement(12092..13264)
                     /gene="cgtA"
                     /locus_tag="JNC24_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HAY9157248.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFILE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(13280..14245)
                     /locus_tag="JNC24_000404"
     CDS             complement(13280..14245)
                     /locus_tag="JNC24_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HAY9157249.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(14372..14629)
                     /gene="rpmA"
                     /locus_tag="JNC24_000405"
     CDS             complement(14372..14629)
                     /gene="rpmA"
                     /locus_tag="JNC24_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007369914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HAY9157250.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(14650..14961)
                     /gene="rplU"
                     /locus_tag="JNC24_000406"
     CDS             complement(14650..14961)
                     /gene="rplU"
                     /locus_tag="JNC24_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HAY9157251.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            15220..16191
                     /gene="ispB"
                     /locus_tag="JNC24_000407"
     CDS             15220..16191
                     /gene="ispB"
                     /locus_tag="JNC24_000407"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HAY9157252.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            16420..16698
                     /gene="sfsB"
                     /locus_tag="JNC24_000408"
     CDS             16420..16698
                     /gene="sfsB"
                     /locus_tag="JNC24_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HAY9157253.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(16746..18005)
                     /gene="murA"
                     /locus_tag="JNC24_000409"
     CDS             complement(16746..18005)
                     /gene="murA"
                     /locus_tag="JNC24_000409"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAY9157254.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(18060..18314)
                     /gene="ibaG"
                     /locus_tag="JNC24_000410"
     CDS             complement(18060..18314)
                     /gene="ibaG"
                     /locus_tag="JNC24_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015585657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HAY9157255.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(18474..18767)
                     /gene="mlaB"
                     /locus_tag="JNC24_000411"
     CDS             complement(18474..18767)
                     /gene="mlaB"
                     /locus_tag="JNC24_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HAY9157256.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(18767..19402)
                     /gene="mlaC"
                     /locus_tag="JNC24_000412"
     CDS             complement(18767..19402)
                     /gene="mlaC"
                     /locus_tag="JNC24_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HAY9157257.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(19421..19972)
                     /gene="mlaD"
                     /locus_tag="JNC24_000413"
     CDS             complement(19421..19972)
                     /gene="mlaD"
                     /locus_tag="JNC24_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HAY9157258.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTEPTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(19977..20759)
                     /gene="mlaE"
                     /locus_tag="JNC24_000414"
     CDS             complement(19977..20759)
                     /gene="mlaE"
                     /locus_tag="JNC24_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HAY9157259.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDTIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(20767..21576)
                     /gene="mlaF"
                     /locus_tag="JNC24_000415"
     CDS             complement(20767..21576)
                     /gene="mlaF"
                     /locus_tag="JNC24_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HAY9157260.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQANPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            21786..22763
                     /locus_tag="JNC24_000416"
     CDS             21786..22763
                     /locus_tag="JNC24_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HAY9157261.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQAERQGTDSLTR
                     EQLAELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAI
                     GTSLPELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIEPLAYSRDY
                     SVMLLVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            22777..23763
                     /gene="kdsD"
                     /locus_tag="JNC24_000417"
     CDS             22777..23763
                     /gene="kdsD"
                     /locus_tag="JNC24_000417"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HAY9157262.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPGEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            23784..24350
                     /gene="kdsC"
                     /locus_tag="JNC24_000418"
     CDS             23784..24350
                     /gene="kdsC"
                     /locus_tag="JNC24_000418"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HAY9157263.1"
                     /translation="MSKAGASLATCYGPVSADVIAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            24347..24922
                     /gene="lptC"
                     /locus_tag="JNC24_000419"
     CDS             24347..24922
                     /gene="lptC"
                     /locus_tag="JNC24_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HAY9157264.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGIDMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPNSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            24891..25448
                     /gene="lptA"
                     /locus_tag="JNC24_000420"
     CDS             24891..25448
                     /gene="lptA"
                     /locus_tag="JNC24_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HAY9157265.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            25455..26180
                     /gene="lptB"
                     /locus_tag="JNC24_000421"
     CDS             25455..26180
                     /gene="lptB"
                     /locus_tag="JNC24_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004091254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HAY9157266.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            26228..27661
                     /gene="rpoN"
                     /locus_tag="JNC24_000422"
     CDS             26228..27661
                     /gene="rpoN"
                     /locus_tag="JNC24_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HAY9157267.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDIGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHINTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            27684..27971
                     /gene="hpf"
                     /locus_tag="JNC24_000423"
     CDS             27684..27971
                     /gene="hpf"
                     /locus_tag="JNC24_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007369896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HAY9157268.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            28089..28580
                     /gene="ptsN"
                     /locus_tag="JNC24_000424"
     CDS             28089..28580
                     /gene="ptsN"
                     /locus_tag="JNC24_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HAY9157269.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVSADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            28626..29480
                     /gene="rapZ"
                     /locus_tag="JNC24_000425"
     CDS             28626..29480
                     /gene="rapZ"
                     /locus_tag="JNC24_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HAY9157270.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            29477..29749
                     /gene="npr"
                     /locus_tag="JNC24_000426"
     CDS             29477..29749
                     /gene="npr"
                     /locus_tag="JNC24_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HAY9157271.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            29963..30595
                     /gene="yrbL"
                     /locus_tag="JNC24_000427"
     CDS             29963..30595
                     /gene="yrbL"
                     /locus_tag="JNC24_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HAY9157272.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKLSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(30592..31320)
                     /gene="mtgA"
                     /locus_tag="JNC24_000428"
     CDS             complement(30592..31320)
                     /gene="mtgA"
                     /locus_tag="JNC24_000428"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HAY9157273.1"
                     /translation="MSKSRLTVFSFVRRFLLLLMVVLAVFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDWVSMDQISPWMGLAVIAAEDQKFSEHWGFDVASIEKA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSSPSGYVRS
                     RQAWILRQMYQLGGEPFMQQHQLD"
     gene            complement(31317..31970)
                     /gene="elbB"
                     /locus_tag="JNC24_000429"
     CDS             complement(31317..31970)
                     /gene="elbB"
                     /locus_tag="JNC24_000429"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HAY9157274.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFATLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(32200..34536)
                     /gene="arcB"
                     /locus_tag="JNC24_000430"
     CDS             complement(32200..34536)
                     /gene="arcB"
                     /locus_tag="JNC24_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HAY9157275.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPVDVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(34632..35561)
                     /locus_tag="JNC24_000431"
     CDS             complement(34632..35561)
                     /locus_tag="JNC24_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312117.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HAY9157276.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLINRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTVHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGLAECLQTLERVVETGVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTE"
     gene            36236..40696
                     /gene="gltB"
                     /locus_tag="JNC24_000432"
     CDS             36236..40696
                     /gene="gltB"
                     /locus_tag="JNC24_000432"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HAY9157277.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGHILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHPGKALYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            40709..42127
                     /locus_tag="JNC24_000433"
     CDS             40709..42127
                     /locus_tag="JNC24_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase"
                     /protein_id="HAY9157278.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGSDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            42311..42790
                     /locus_tag="JNC24_000434"
     CDS             42311..42790
                     /locus_tag="JNC24_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001613919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 family protein"
                     /protein_id="HAY9157279.1"
                     /translation="MESLSERTSTGYQQIHDGIIHLVDSARTETVRSVNALMTATYWE
                     IGRRIVEFEQGGEARAAYGAQLIKRLSKDLSLRYKRGFSAKNLRQMRLFYLFFQHVEI
                     RQTVSGELTPLGIPQTPSAEFPSAKIWQTLSAKLRGCLIFPQLLYCISYFRFVYSCK"
     gene            42807..43112
                     /locus_tag="JNC24_000435"
     CDS             42807..43112
                     /locus_tag="JNC24_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY9157280.1"
                     /translation="MIYIEQLELIHKSGDVLYPVKITRKSSGKTAFHLVPFGLNKTHD
                     LLEVEDASEAIRLVIDERHSIRCSTLTATITNKKGKRIKRTGIYSIKGVNIKEYNVR"
     gene            complement(43188..43652)
                     /locus_tag="JNC24_000436"
     CDS             complement(43188..43652)
                     /locus_tag="JNC24_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF386 domain-containing protein"
                     /protein_id="HAY9157281.1"
                     /translation="MMMGEVRSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSTIENEQAIILKPGMFAVFMLGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(43649..44524)
                     /locus_tag="JNC24_000437"
     CDS             complement(43649..44524)
                     /locus_tag="JNC24_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HAY9157282.1"
                     /translation="MTTLAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDVTEMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELMGADARTIFTRAGQGDEQAQQLIHRSA
                     HVLARLIADIKATTDCQCVVVGGSVGLAEGYLALVETYLAQEPAVFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(44521..45210)
                     /locus_tag="JNC24_000438"
     CDS             complement(44521..45210)
                     /locus_tag="JNC24_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAY9157283.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPIPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSEAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(45258..46748)
                     /gene="nanT"
                     /locus_tag="JNC24_000439"
     CDS             complement(45258..46748)
                     /gene="nanT"
                     /locus_tag="JNC24_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HAY9157284.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(46858..47751)
                     /gene="nanA"
                     /locus_tag="JNC24_000440"
     CDS             complement(46858..47751)
                     /gene="nanA"
                     /locus_tag="JNC24_000440"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HAY9157285.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(47873..48664)
                     /gene="nanR"
                     /locus_tag="JNC24_000441"
     CDS             complement(47873..48664)
                     /gene="nanR"
                     /locus_tag="JNC24_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312126.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HAY9157286.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGEFSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVADQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
     gene            49044..50366
                     /gene="dcuC"
                     /locus_tag="JNC24_000442"
     CDS             49044..50366
                     /gene="dcuC"
                     /locus_tag="JNC24_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HAY9157287.1"
                     /translation="MFGIIISVIVLITMGYLILKNYKPQVVLAAAGVFLMMCGVWLGF
                     GGVLDPAKSSGYLIVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERIGASRAMVSL
                     LSRPLKLIRSPYIILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAV
                     AVIATTMSIEWGILETNSIFAAQVAGMKIATYFFHYQLPVASCVIISVAISHFFVQRA
                     FDKKDKNINHEQAEQKTLDNVPPLYYAILPVMPLILMLGSLFLAHVGLMKSELHLVVV
                     MLLSLTVTMFVEFFRKHNLRETMDDVQAFFDGMGTQFANVVTLVVAGEIFAKGLTTIG
                     TVDAVIRGAEHSGLGGIGVMIIMALVIAICAIVMGSGNAPFMSFASLIPNIAAGLHVP
                     AVVMIMPMHFATTLARAVSPITAVVKRTAIPMAVGFVVNMIATITLFY"
     gene            complement(50409..50906)
                     /gene="sspB"
                     /locus_tag="JNC24_000443"
     CDS             complement(50409..50906)
                     /gene="sspB"
                     /locus_tag="JNC24_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HAY9157288.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(50912..51550)
                     /gene="sspA"
                     /locus_tag="JNC24_000444"
     CDS             complement(50912..51550)
                     /gene="sspA"
                     /locus_tag="JNC24_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein A"
                     /protein_id="HAY9157289.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIELSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            complement(51945..52337)
                     /gene="rpsI"
                     /locus_tag="JNC24_000445"
     CDS             complement(51945..52337)
                     /gene="rpsI"
                     /locus_tag="JNC24_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAY9157290.1"
                     /translation="MAENQYYGTGRRKNSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            complement(52353..52781)
                     /gene="rplM"
                     /locus_tag="JNC24_000446"
     CDS             complement(52353..52781)
                     /gene="rplM"
                     /locus_tag="JNC24_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HAY9157291.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQVSFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            complement(53000..54127)
                     /locus_tag="JNC24_000447"
     CDS             complement(53000..54127)
                     /locus_tag="JNC24_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HAY9157292.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSTP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFARGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLHDETRAQMDKLWLALAGGKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGDRLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            54321..54719
                     /locus_tag="JNC24_000448"
     CDS             54321..54719
                     /locus_tag="JNC24_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1043 family protein"
                     /protein_id="HAY9157293.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            54873..56240
                     /gene="degQ"
                     /locus_tag="JNC24_000449"
     CDS             54873..56240
                     /gene="degQ"
                     /locus_tag="JNC24_000449"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HAY9157294.1"
                     /translation="MKKQPQLLSALALSVGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPEQPAQPFEGLGSGVIINASKG
                     YVLTNNHVINQAQKISIQLNDGREFDAKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIVSALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPGSGSAKAGVKAGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTSTSSSASAEMIAPALEGATLSDGQLKDGGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKSAIIALQIVRGNESI
                     YLLMR"
     gene            56330..57397
                     /gene="degS"
                     /locus_tag="JNC24_000450"
     CDS             56330..57397
                     /gene="degS"
                     /locus_tag="JNC24_000450"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HAY9157295.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQGGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
BASE COUNT        14265 a        15301 c        14255 g        13610 t
ORIGIN      
        1 agcgcattat tttacaaaaa acacactaaa ctcttcctgt ctccgataaa agatgattaa
       61 atgaaaactc atttattttg cataaaaatt cagtgagagc ggaaatccag gctcatcatc
      121 agttaattaa gcagggtgtt attttatgac gacgattctc aagcatctcc cggtaggtca
      181 acgtattggt atcgcttttt ccggcggtct ggacaccagt gccgcactgc tgtggatgcg
      241 acaaaaggga gcggttcctt atgcatatac tgcaaacctg ggccagccag acgaagagga
      301 ttatgatgcg atccctcgtc gtgccatgga atacggcgcg gagaacgcac gtctgatcga
      361 ctgccgcaaa caactggtgg ccgaaggtat tgccgctatt cagtgtggcg catttcataa
      421 caccaccggc ggcctgacct atttcaacac gacgccgctg ggccgcgccg tgactggtac
      481 catgctggtt gctgcgatga aagaagatgg cgtgaatatc tggggtgacg gtagcaccta
      541 taaaggaaac gatatcgaac gtttctatcg ttatggtctg ctgaccaatg ctgaactgca
      601 gatttacaaa ccgtggcttg atactgactt tattgatgaa ctgggcggcc gtcatgagat
      661 gtctgaattt atgattgcct gcggtttcga ctacaaaatg tctgtcgaaa aagcctactc
      721 cacagactcc aacatgcttg gtgcaacgca cgaagcgaag gatctggaat acctcaactc
      781 cagcgtcaaa atcgtcaacc caattatggg cgtgaaattc tgggatgaga gcgtgaagat
      841 cccggcagaa gaagtcacag tacgctttga gcaaggtcat ccggtagcgc tgaacggtaa
      901 aacctttagc gacgacgtag aaatgatgct ggaagctaac cgcatcggcg gtcgtcacgg
      961 cctgggcatg agcgaccaga ttgaaaaccg tatcatcgaa gcgaaaagcc gtggtattta
     1021 cgaagctccg gggatggcac tgctacatat cgcttatgaa cgtctgttga ccggtattca
     1081 caacgaagac accattgagc agtatcatgc acatggtcgt cagttgggcc gtctgctgta
     1141 ccaggggcgt tggtttgatt cccaggcgct gatgctgcgt gactctctgc aacgctgggt
     1201 tgccagccag atcactggtg aagttaccct ggagctgcgc cgtgggaacg attattcaat
     1261 cctgaatacc gtctcagaga acctgaccta caagccagag cgtctgacga tggaaaaagg
     1321 cgactcggtg ttctcgccag atgatcgtat tggtcaattg accatgcgta acctggatat
     1381 cactgatacc cgagagaaac tttttggtta tgccaaaact ggcctgctct cctcctctgc
     1441 cacttcaggc gtgccgcagg tggagaatct ggaaaacaaa ggccagtaat tcgacatcaa
     1501 ccctgccctt caggggtggg gttaccttgc ttatcctctg taaactcctt tctgagcatc
     1561 acataatcga aaacatcatg gtttgcatcc acaacccgtg agtctccttt atagtgtgca
     1621 atcacttcct ccagcgtttt cggttgttcc ggctttgtta cccccatcca cgcattaata
     1681 aggtaattat tgtaagctgt cgaaaagatg ttgttttccg ttgtacgatc cacgccatcc
     1741 ccaagaacgg ggctatacca gataaacatc gggacatgat atgcctgctg gctagcctcc
     1801 ctgcctccat gaaaatagac gttcttcttc gtagggtcac gttccaggcc gtggtcggca
     1861 aaatacatga ccgaggcgcg gcgatctttt aataattcaa aaacctgacc tagcaaacta
     1921 tctgtgtaat gaatggagtt gtcatagcag gcatcctgat cgtcctgcgg ttgaaacacg
     1981 gcgctggatt gcggataggc gctacaagcc ggttcatggc ttccatttaa atgaagaaca
     2041 atcagtttct tttgctgcgt attttgctgt aatgcttgcg ataaatgcgg caacaacaat
     2101 tcatcaaatc ctctgacata aactgtttcc atggcgcgca tggcgatact ggtaactgct
     2161 gtaccattct gccgaaaagc ggattgcgag cttagccaga aagtctgaaa tcctgcttga
     2221 ttagccatat taataatgtt gtcggggtaa ttatgaatgt catgactcaa aacagaatca
     2281 gcagttaaag aaaggggaac cgacagcgca gtgtaaggtg cgccgcttat tgattgatta
     2341 aacagtttga tctgttttct ttgtgcttca acttgcggtg tcgtagagcg tgtatatcca
     2401 tacaaagaca tattgtcgac acgtacagac tcccccacaa tcaacacgta ggtatcaata
     2461 cctgtatccc tgactgataa ttgaaaatac ggcaccgtgt ttgcaattga gagtaatctt
     2521 tgatgctctt tcgctgctaa agcaaaataa ttgagattga aaaacggcgt ataggtagca
     2581 aatcgcgacg ctagtatata tggactgaat gcttttttat tttttgcatc tttataagca
     2641 aattgacaag cagaaaataa actgcccgaa atgacaatca gcaataatat tcctgtcact
     2701 ttttttgtcg gcaaggaaac atcatatttt attattactg ccaaaaaaag aagggataaa
     2761 aaagcaaaag cacatagata agggatatac atccccagca ttttgacaac ttcatccgga
     2821 tcactctgca gcacactaat cgcaaatcca tcattgaaag ttgtaccaaa ggtacaccat
     2881 gaatagaggc taatactcat gtctgccgca gtcagtaaca caaatggtgc ggcaacaagc
     2941 aggcgaagcg gaagggatga tatacgtttt acaaccagga tggtcaggac gaagaacagc
     3001 aatcgctgaa ccatatgcgc gccggaagag gcgactaagt tcgtaatacc aaaaacaata
     3061 acacaaagat acaacaacca atgagattta aaatcttcta gcaaatttgt tgaatactgt
     3121 catccagaaa tccaagttca caaccaatat aaacaattat caacaatgtt caattttata
     3181 tcagacatgt ttttcaactc tgatccatat cagaccgaaa cactgttagt tttttctttt
     3241 taaatgatgc ggccattgcg ttctcagtaa ttaaataaca ttatcaatgc gtctctaaac
     3301 attacgcaga acgtagccat cagtagttat actgacagga gcaggatggt ttatcgagaa
     3361 gtaaacatat ccgtattcgt accggaactg gagttgacac aataaagtgc caattatgtc
     3421 agcagaaggg aaaagtttat aactaaggcg taccggcacc atcgtttcaa ggtaccagct
     3481 acgagtaaag caactggacg agatacagat acctgacaac cattcctcag accaggacca
     3541 aaacgaaaaa agacgctttt cagcgtctct tttctggaat ttggtaccga ggacgggact
     3601 tgaacccgta agccctattg ggcactacca cctcaaggta gcgtgtctac caattccacc
     3661 acctcggcac ggatactact ttttagttcg ggatatcgct ggtcggctta gccggagcag
     3721 ctggctgagt ttgttcggtt ttcgccggtg cactcagatt ttcccattcg ctacctttat
     3781 tggttttgtt gctattgatg ttacccagca ccagactgat gataaagaat aacgttgcca
     3841 gcagcgccgt catgcgggtc atgaagttac cagaaccact tgaaccaaac agcgtagcgg
     3901 aagcgcctgc tccgaaggag gctcccatat cagcgccttt accttgctgc agcatgatca
     3961 gaccaacaag gccaattgcc acaataagga aaactaccaa aagagcttcg tacataatca
     4021 acctgttcct tgcggagttg ccgcgtacca atgcttcaac caataaagcg ggagttttta
     4081 atctttccca ctgaagtggg tgtgaatact aaccaaagcg aatgaccttc gcaagggcaa
     4141 ttttatcgca ttgtatcaac tgcggaaaaa aacagcaaaa ccgatgtgtt cgctgagaaa
     4201 aaaggcgaca atcgccgcct ttttagccag ttatttaccg ttttaaacgg cttttactgc
     4261 atcggcgata cggtgtgcaa attcagttac ctgcgcttcg tcttcacctt ctaccatcac
     4321 gcgaattaac ggttcggtgc cggatttacg caacagcaca cgtccacggc tacccagcgc
     4381 cgcttcaact tcggcggtca cagctttaac tgactcatgc tcaagcggat cgccgctacc
     4441 tgcggtgtaa cgcacgttaa ccagaatctg cgggaacatt ttcatgccgc tgcaaaggtc
     4501 gtgcaggctc atatggttac gtgccatcgc cgccagcacc tgcaagccag caacgatgcc
     4561 gtcaccggta gtggttttat ccagcaggat cacatgaccg gaattctctg caccgatacg
     4621 ccagcctttc tcctgcattt tttccagtac gtagcggtca cccactttcg cgcgcgcaaa
     4681 tggaattcct aactgtttca acgccagctc aagccccatg ttactcatca atgtacccac
     4741 agcaccacca cgcagctggc cctgacgaag accttcacgc gcgatgatat acatgatctg
     4801 atcaccatcg actttattgc cttcatggtc aaccataatc acgcgatcgc cgtcgccgtc
     4861 gaaggcaata ccgagatccg ctttttcagc cagcacacga gcctggagcg cgcgaacgtc
     4921 ggtagccccc acttcggcat tgatgtttac accgttcggc tcacaaccga tagcgataac
     4981 gttcgccccc agttcacgca gcacgttcgg cgcgatgtga taagtcgcac cgtttgcgca
     5041 atccaccaca atcttcagtt cgctgaggct aagttcgttc gggaacgtgg ctttgcaaaa
     5101 ctcgatatag cgacccgcag catcaacgat acggctggct ttacccagtt ctgccgaatc
     5161 aacacagctg atctcttttt ccatttccgc ttcgatggcc tcttctaccg catccggtag
     5221 tttggtgccg tcgatagaga aaaatttaat gccattatcg tagaacgggt tatgcgatgc
     5281 agatatcaca attccggcct ctgcgcggaa ggtacgcgtc agataagcca cggccggtgt
     5341 tggcatcggg ccagtgaaga gtgcggaaag gcccgctgcc gccagacccg cttccagtgc
     5401 tgactccagc atatagccag aaatacgcgt gtctttacca ataatgatct tacgggagcc
     5461 gtggcgcgcc agcactttac ccgcggccca acccagctta agcacaaaat caggtgtgat
     5521 cggcgcatcc cctacacgac cacgaatccc atcggtaccg aaatatttac gattactcat
     5581 agcgtttgtt ttcctttgca gacagagtgg cttccaccac ccgcatcgct tctacggttt
     5641 ctttgacgtc atgaacacga ataatgtgcg cgccttgcat tgcggcaatg accgcacagg
     5701 ccagactacc gctcaggcgc tcggacggcc ccacgttcag cagctgccca atcatcgatt
     5761 ttcgtgacat acctaccaac agcggcaggt tgaaatggtg aaattcagcc aggcgcgcca
     5821 gtaatgaata gttatgggag agatttttac cgaaaccgaa tccggggtcg agcaacaatt
     5881 tctcttttgc gatacccgcc tgctcgcaac gtgctatttg ctcaataaag tagcgattca
     5941 cttctgcaaa gacatcgtca tacttcggag cttcctgcat ggtttttgga tttccctgca
     6001 tatgcatcag acaaaccggt aaaccggttt ctgcagccgc ctccagagcg ccaggttcgg
     6061 aaagggagcg gatatcgtta ataatgtgag cgccaacttt cgctgactca cggatgactt
     6121 ctggtttgga tgtatctacc gagatccaga cttcgaagcg ttgagcaatt gcctcaacca
     6181 caggaataac acgttgcaac tcttcttcaa cgctaacttc cgccgcccct gggcgcgtgg
     6241 actcgccacc aacgtcaatg atcgtcgcgc cagcgttgat catcagattc gcatgtttca
     6301 ccgcatctat cagcgagtta tgcgtgccac catccgaaaa ggaatcaggc gtgacgttga
     6361 ggatccccat tacgtgagga tggctaaggt ccagtgaagt gccctgggca aagagtttca
     6421 tggtgttatc cctggtatga attgataaga aaaaaccccg gagcacgccc cggggttttc
     6481 ggtacaaata cagtcatctg atgcgggaac ttacttgtcg cctaactgct ctgacatggt
     6541 gttacccggg ttcggcgtac gcggttcatc aaccggacga ggagcctttg gactaccatt
     6601 gtcgccagaa ttgttagaag cgcctggttc ttcccagccc gctggcggac gtacatcgcg
     6661 acgtgccatc aggtcatcaa tctgcggtgc gtcgatagtc tcatatttca tgagagcatc
     6721 tttcatcgca tgcagaatat ccatattgtc ggtcagaagc tgacgcgcac gattatagtt
     6781 acgctcaatc agtgctttca cttcctggtc gatgatacgt gcagtttcat cggacatatg
     6841 tttcgctttc gctacgctac ggccgaggaa cacttcacct tcttcttccg cgtacagcag
     6901 tggacccaat ttctcagaga agccccactg agtcaccatg ttacgcgcca ggttggtcgc
     6961 aactttaata tcgttggacg caccggtaga aacatgttcc ggcccgtaga tgatctcttc
     7021 tgccagacga ccaccgtaca gcgtagaaat ctggctttcc agtttctgac ggctggcgct
     7081 gattgcgtcg ccctcaggca agaagaaagt cacacccagc gcacgaccgc gtgggataat
     7141 cgtcactttg tgcaccggat cgtgttccgg caccaggcga ccgataatcg catgacccgc
     7201 ttcgtggtaa gccgtcgatt ctttctgcgc ttccgtcatc accatggagc gacgttccgc
     7261 acccatcatg attttatctt tcgctttctc gaactcaacc atcgacacaa cgcgtttgtt
     7321 gccacgagca gcgaacagtg ccgcttcgtt taccaggttc gccaggtcag caccggagaa
     7381 accaggagta ccacgggcaa tgattgccgc gtcgatatcg ggtgccaatg gtacgcgacg
     7441 catgtgaact ttcaggatct gctcacgacc gcgaacatct ggcaagccaa ccacaacctg
     7501 acggtcgaaa cggccaggac gcagcagggc cgggtcaaga acgtccggac ggttagtcgc
     7561 ggcgataacg atgatacctt cgttaccttc gaagccatcc atctcaacca gcatctggtt
     7621 cagagtctgt tcacgttcat cgtgaccacc gcccagacca gcgccacgct ggcggcctac
     7681 ggcgtcgatt tcatcgataa agatgatgca cggtgccgct ttcttcgcct gttcgaacat
     7741 gtcacgaaca cgggatgcac ccacaccgac gaacatttct acgaagtcag aaccggagat
     7801 agtaaagaac ggaactttcg cttcgcctgc aatcgctttc gccagcagcg ttttaccggt
     7861 acccggagga ccgaccatca ggacgccttt cgggatctta ccgccgagtt tctggaagcg
     7921 gctcggctcg cgcagatatt caaccagttc agcaacttct tcttttgctt cgtcgcagcc
     7981 cgcaacgtca gcaaaggtcg ttttgatctg atcttccgtc agcatgcgcg ctttgctctt
     8041 accaaacgac atggcacctt tgccaccgcc gccctgcatt tgacgcatga agaagatcca
     8101 cacaccaatc agcaacagca tcgggaacca ggagatgaag atagaagcca gcaggcttgg
     8161 ttcttcaggc ggttcaccga caaccttgac gttcttggtc aacaggttat ccagtaattt
     8221 cggatcctga accggaatgt aagtggtata acggttacta tctttcttgg taacgttgat
     8281 ttcacgtccg ttgatacgcg cttcacgaac ctggtcgtta ttgacctctt gtaggaaggt
     8341 agagtaatcc accttacggc cattagactc gctgggccca aagctctgga atactgacat
     8401 cagcacaacg gcaatgacca gccagagtat taggtttttc gccatgtcac tcaagggatt
     8461 aacctcttat tacaactgtg ttaaaaacag cgtcaggata ctctatatcc agcatctttc
     8521 aaactttcgt ctgaaatctc ccggttaggg tttacgcccg gtcgctacaa tatacacttc
     8581 ccgcgaacgt gcacgagaag agtccggctt acgaactttg accttcgtaa acagggagcg
     8641 aatttccctt agatactcat cgaaaccttc gccctggaac accttcacta caaaactgcc
     8701 acctggcgct aatacatcac gacacatttc tagcgccagt tccaccagat acatggcacg
     8761 ggggatatcc accgccggtg ttccgctcat gtttggtgcc atatcggaca tgacaacctg
     8821 gactttgctg tcgccaacgc gctccagcag tgctttcatc accagttcat cacgaaaatc
     8881 gccctgaaga aagtctacac caacgatagg atccataggt aaaagatcgc aagcgatgat
     8941 gcggcctttg ccgccaattt gggtgaccac atattgtgac caaccacccg gagcagcacc
     9001 aaggtcgaca accgtcattc ccggtttaaa gagtttgtca ctttgctgta tttcatcaag
     9061 tttaaaccag gcacgggaac gtaacccctt tttctgtgcc tgttgaacat atttatcgct
     9121 aaagtgttcc tgaagccagc ggctggagct ggcagaacgc ttcttacctg tcatttaact
     9181 ttcccatggg gataactcat cgtaaccaat tgcgtaaatt tttacgcgcc tatttggtga
     9241 tatatgggag atggcggtag aatgacccat tttcaatccc aacgtaagca aaaaatacga
     9301 tgaatctgag tactaaacaa aaacagcacc tgaaaggtct ggcacatccg ctcaagccag
     9361 ttgttctgct tggcagtaat ggtttgaccg aaggggtgct ggccgagatt gaacaagcgt
     9421 tagagcacca tgaactcatc aaggtgaaaa tcgccaccga agatcgcgaa acaaaaacct
     9481 tgatcgtgga agctatcgtg cgcgaaaccg gcgcctgtaa tgtacaggtc atcggtaaaa
     9541 cgctggtgct ttatcgccca actaaagaac gtaaaatctc gctgccacgc taagattatc
     9601 ctaaagttac acacatttgc tgtgtaaaac gaggggtttt ccgcaggcag gagagcaaaa
     9661 tgccacgctc tgttcgttga taaaaggccg catagcggcc tttttccttt ctttacaata
     9721 catcaacatc ttgagtattg ggtaattctt acaggtattc caccttaatt acttcaaatt
     9781 ctacttcgcc gcccggcgtt ttgatgacca caacatcatc ttcttctttg ccgatcaggc
     9841 cacgagcaat aggcgagttt acagaaatca ggttttgttt aaagtcagct tcgtcatcgc
     9901 caacgatgcg ataagtctgt tcttcgtcag aatccagatt cagcaccgtt acggtagcac
     9961 caaaaataac gcgcccattg ttaggcattt tggtgacatc aatcacctgc gcgtttgaca
    10021 gcttggcttc gatgtctttg atacggcctt cgcagaagcc ctgctgttca cgagctgcgt
    10081 ggtattcggc gttttctttc aggtcgccat gctcacgcgc ttccgcgata gcagcaatga
    10141 tttcaggacg gcgcacagat ttcagaaaat ccagctcttc gcgtaatttt tcagcgccgc
    10201 gtaaggtcat cggaatagct tgcatttgtt atacctcttg aatattcctg atggggcaag
    10261 tcttcaccca ccccggctgt tcggcctgcc cggcataact cctcaccggg accagaagca
    10321 aaaaaatacc gacccaggta caagtcccag gtcagctaca attcacattt tgatagtcat
    10381 tttaccctga agttcccgaa gggtcatcgt ttactttata gggcgttgcg ccgtagtatg
    10441 acggctcgat tccaggttgt tagcgcgaga ttatgcgatt ttccagattt atcatcggat
    10501 tgaccagctg tatagcgttc agtgttcagg ccgcaaatgt tgatgagtac attactcaac
    10561 tccccgctgg tgccaacctt gccctgatgg tgcaaaaagt cggcgcgtcg gcccccgcta
    10621 ttgattacca cagtcagcag atggcgctgc ctgccagtac ccagaaagtg attactgcgc
    10681 tggcggcgtt gattcaactc ggccccgatt ttcgttttac cacgacgctt gaaaccaaag
    10741 gcaatgtgga aaacggcgta cttaagggtg acttagtggc gcgatttggt gccgatccga
    10801 cgttaaaacg tcaggatatt cgcaatatgg tcgcgacttt gaaaaaatct ggcgtcaacc
    10861 aaatcgatgg caatgtgttg atagatacct ccattttcgc cagccacgat aaagcccccg
    10921 gctggccatg gaatgacatg acacaatgct ttagcgctcc gcctgccgcc gccatagttg
    10981 accgcaactg tttctccgtc tcgctctaca gtgcccaaaa acccggtgat atggccttta
    11041 tacgcgtggc atcttattac cccgttacga tgttcagcca ggtacgcacc ctcccccgtg
    11101 gttctgccga agcgcaatac tgcgaactgg atgtcgtacc aggcgacctg aaccgcttta
    11161 cgctgacggg atgcctgcca caacgttctg agccgctccc gttggctttt gccgtgcagg
    11221 atggagccag ttatgccggt gcaattctga aagatgagtt aaaacaggcg ggtatcacct
    11281 ggagcggaac actgctgcgc cagactcagg ttaacgaacc tggaacggta gttgccagta
    11341 aacagtcggc cccgctgcac gatctgctta agattatgct gaaaaagtcg gataacatga
    11401 tcgccgatac ggttttccgc atgataggcc atgcgcgctt caatgtacct ggtacatggc
    11461 gggccgggtc ggacgctgtg cgtcagatcc tgcgccagca agccggtgtc gatattggaa
    11521 acaccattat tgccgatggt tcagggcttt cgcggcataa cctgattgcc cccgccacca
    11581 tgatgcaggt gctgcaatac attgcccaac acgacaatga acttaacttt atctccatgc
    11641 tgccgctggc gggctatgac ggctctttgc agtaccgtgc aggtctgcat caggcgggcg
    11701 tggatggaaa agtctcagcg aaaaccggtt cgttgcaggg ggtatataac ctggcgggat
    11761 tcattaccac agcgagcggg caacgaatgg cgtttgtgca atatctttct ggctatgcag
    11821 tagaacctgc ggatcagcgt aatcgccgta ttccgttagt gcgttttgaa agccgtttgt
    11881 ataaagatat ttatcagaac aattagtcaa aaagaaaccc cggcacatgg ccggggcttc
    11941 agattattga caaagtgcgc tttgtttatg ccggatgcgg cgtaaacgcc ttatccggcc
    12001 tacaaaatcg tgcaaattca acatattgca attctcttgt aggcctgata agcgtagcgc
    12061 atcaggctga tttggcgttt atcatcagtg attaacgctt gtaaatgaac tcaacgcctt
    12121 cttcgtcgtc ttcgtcccag tcgtcatccc agtcttcatc atcctcttca gcaatctctt
    12181 caagctgctg gcgatgataa tcgtcccaca tgaattcgac tttctctggc tgtttcgctt
    12241 cttcagcctg cacgaccggg ttttcaagga taaaggtcat cacatcccag cagagatctt
    12301 tcacgcccag tccgctcgcc gcagagatca gataatattt atcttcccag cccagcgctt
    12361 cagcaatcgc tttcgctttc tcttcagctt ctgccttatc cagcagatcg atcttattga
    12421 acactaacca acgcggtttc gccgccagat cctggctgta tttttccagc tcgctgataa
    12481 taatacgcgc gttttcaacc ggatcggtgc cgtcaatcgg atcgatatcg atgaggtgca
    12541 acaggacgcg gcaacgttcc aggtgcttca ggaagcggat acccagacct gcgccttccg
    12601 cagcgccttc aatcagtcct ggaatatcgg caacaacgaa gctcttttcg ttgtccattc
    12661 gtaccacacc cagacttggc accagagtgg taaacggata atccgccact ttcggtttag
    12721 ccgccgatac cgcgcgaata aaggtcgatt tacccgcgtt tggcatcccc aacataccga
    12781 cgtcagccag cagcatcagc tccagcagca gctcacgctt atcacccggc gtaccgttgg
    12841 ttttctgccg tggtgtacgg ttaacggacg atttgaaacg ggtattgccc agaccgtgcc
    12901 agccgccctt agcaaccagc agacgctgac cgtgtttagt catatcgccc atggtttcac
    12961 cagtaccctg gtcgattaca cgcgtaccta ccggcacttt aatcgtcacg tctttaccgc
    13021 gcttaccggt acaatcgcgg cttgcgccgt tctgaccgcg ctctgcacgg aaagattttt
    13081 caaaacgata atcgataagc gtgttcaggt tttcgtcggc ttccatccat acgtcaccac
    13141 catcaccacc gtcgccgcca tccgggccgc ctttcggaat atacttttcg cggcggaagc
    13201 tcacgcaacc attaccgcca tcacctgcaa cgaccagaat cgatgcttca tcaacaaact
    13261 tcattttatt ctccataaat cattcgcctg cgcggggttg cgataccacc gttgtatgct
    13321 tacgtaatcc gccccaaata cgatgaccaa tggcggaata catcgcgccc gcaaccacga
    13381 caaacgcacc gagataacct aaaaggttta acatcggtct ggcgaagaaa tcgggccagg
    13441 ccagtgataa aagatctgaa aaaaacagcg taaacagtgg ggtgagcgtg atgatcgcgc
    13501 tcacctgcgc tgcctgccag cgagccatcg cttccgccag ggcgccatat cctaccaatg
    13561 tattcagtcc gcaaaaaatt aaacatgcga gctgccagtg gctaagctgc gctatcactc
    13621 caggctttgc cagagggaag agcgcaattg tacataaagt gtacagtaaa aacaggatct
    13681 gcggtgaggc cagccgacgc aataaaacct tttgcgccac gccataactc acccaaaccg
    13741 tcgccgcacc gaccccaaag ataactcccc aggtgtaatc ggtgagcttt gtaaatatct
    13801 cgaccagact ggtgttaaaa aacatcacca ggccgctcag gagcatcaat gcccctacaa
    13861 cctgagtgct gcgcattttc tctttcagga taaatacgct ggcaaccatc atgccaactg
    13921 gcgagagttg cccaatcacc tgcgaagcgg tcggactcag gtattgcaag gatgagctga
    13981 acaggatgaa gttcccaaac agcccggcgg tcgccactgc caacaaaatc aaccagcgtg
    14041 gcttacgaaa cacgcgtaat ggcggcaacc tcttcttcac cgcaagaatg gcaccaaggc
    14101 caatactcgc catcaagaaa cggtaaaaca cgattgtcgg aggttccatc acctccagca
    14161 cctgcttcat tgcgattggc aacgcccccc agcaaattgc tgtggtgagc gccaaaagaa
    14221 tgccaatgcc tgcctgctgc ttcataccgt attccctgca aagcgcattt gcggattaac
    14281 gtcgcaccat gcgcattttt accggttatc gaatgtaaaa agccccgcaa cgtgttgcgg
    14341 ggctttcatc cgttaccggg acgcgaaaaa cttattcagc ttcgatgctg ataaatttac
    14401 ggtttttcgg gcctttaact tcgaatttca ctttaccgtc tgctttagca aacagagtgt
    14461 ggtcacgacc gcaacctacg ttagcgccag cgtggaattt ggtaccacgt tgacgaacga
    14521 tgatgctacc cgccagaacg gattcgccac cgaaacgctt aacgcccagg cgtttagctt
    14581 ctgaatcgcg accgttacgt gtggagccgc cagccttttt atgtgccatt tgaaatctct
    14641 cctcaggtct taggcgctga tgccagtaat tttcacatca gtgaaccact gacgatgacc
    14701 ctgctgctta cgatagtgtt tacgacgacg aaacttaacg attttaactt tctcgccacg
    14761 accgtgagca acaacttcag ctttgattac gccgccatca acgaaaggaa cgccgatttt
    14821 gacttcttca ccgtttgcga tcatcagcac ttcagcgaac tcaacagttt cgccagttgc
    14881 gatgtccagc ttttccaggc gaacggtctg accttcgctt actcggtgtt gtttaccacc
    14941 actttggaaa accgcgtaca tataaaactc cgcttccgcg cacacctttt caatgattca
    15001 gagtgcgcta taaatattca caatagggcg cgaatattac gcaaaacgca cgcctttgac
    15061 aagtgctaca gtcaatacac gaagaaaaaa aacacaactt gtacggtaac gtttatctgt
    15121 gccatttttt cagtacaatc accctatatt cctaaccata aaccctaagt tgcctttgtt
    15181 cacagtaagg taatcggggc gaaaagcccg gcttttgcga tgaatttaga aaaaatcaat
    15241 gagttaaccg cgcaagatat ggcgggtgtt aatgcggcaa tccttgagca gcttaattcc
    15301 gacgtccaac tgatcaatca gttaggctat tacatcgtca gcggcggcgg taaacgtatt
    15361 cgtccgatga ttgctgtact ggctgcacga gctgttggct atgagggaaa tgcgcatgtc
    15421 accattgctg ccctgatcga gtttatccac acggcgactc tgctacacga cgacgttgtg
    15481 gatgaatcag atatgcgcag gggtaaagct accgccaacg ccgcatttgg caatgccgcc
    15541 agcgtgctgg taggcgattt tatttatacc cgcgcgttcc agatgatgac cagcctcggt
    15601 tcactcaaag tgctggaagt catgtcagaa gctgtaaacg tcatcgcaga aggtgaagtt
    15661 ctgcaactga tgaacgttaa cgatccggac atcactgaag aaaactacat gcgcgttatc
    15721 tatagcaaaa ccgcgcgtct gtttgaggct gccgcgcagt gttccgggat tctggctggc
    15781 tgtacgccgg aggaggagaa aggcctgcag gattatgggc gctatctcgg cactgctttc
    15841 cagttgatcg acgatttact cgattacaat gccgatggcg aacagttagg taaaaatgtc
    15901 ggcgacgatc tgaacgaagg taaaccgacg ctgccgctgc tgcatgcgat gcatcatggc
    15961 acaccagaac aggcacagat gatccgtacc gccatcgaac agggtaacgg tcgccatctt
    16021 ctggaaccgg ttctggaagc aatgaacgct tgtggatctc ttgaatggac gcgtcagcgt
    16081 gccgaggaag aagcagacaa agccatcgca gcattacagg tgctcccgga caccccttgg
    16141 cgagaagcac tcatcggcct cgcgcacatc gctgttcaac gcgatcgtta atcccctccc
    16201 ttcatcccgc gcagtgcgcg ggatgattcc agtaaattcc ataaaaacac taattcagcg
    16261 ctataacacc tgaaaaaaaa gggcgatgaa tctttatata ttctgaatat tcacactctt
    16321 tacaggaact ttttagagca ataggccatc aggagtatag tgatgcccga cagaagaagt
    16381 gttctgaatg aaagcgacta acttaaggag tgaggaaaga tggaaagtaa tttcattgac
    16441 tggcatcccg ctgacatcat tgcgggtttg cgcaagaagg gaacgtcaat ggcggcggag
    16501 tctcgcagaa atggtttgag ttcctcaacg ctggcaaacg cattatcgcg cccatggccg
    16561 aaaggagaga tgattattgc gaaagccctg ggaactgacc cctgggttat ctggccatca
    16621 cgctaccatg atccgcagac ccatgagttt atcgacagaa cgcagttgat gcgtagctac
    16681 actaaaccga aaaaatgagt ggtctggcgg tagccccgcg aacggggctg ccagctctca
    16741 gacgattact cgcctttcac acgctcaata tttgcaccta aagcgcgcag tttgtcttca
    16801 atgcgttcgt agccacgatc gatgtgataa atacgatcaa ccaccgtcgt cccttccgca
    16861 atacagccag ccagcaccag gcttgctgat gcacgcagat cggttgccat aacctgtgcg
    16921 ccagaaagtt tttcaacacc gtgacaaata acggtattgc tttcgatttc ggcatgcgcg
    16981 cccatacggc taagctccgg cacatgcata aagcggtttt caaagaccgt ttcggtgatg
    17041 aatccggtcc cttctgccac caggttcaac agcgtgaact gggcctgcat atcggtcggg
    17101 aatgccggat gcggcgcggt acgtacgtta acagccttcg gacgtttgcc atgcatatcc
    17161 aggctaatcc agtcttcgcc gacttcgatg tccgctccag cgtcacgcag tttcgccagc
    17221 acggcgtcga gagtatctgg ctgcgcgtta cggcagataa ttttgccgcg agaaatcgcc
    17281 gccgccacca ggaaagtacc ggtttcgata cgatccggta gcacgcgata gacaccgccg
    17341 cctaaacgtt ccacaccttc gatgacgata cgatcggtgc cctgaccgct aattttcgca
    17401 cccagcgtaa tcaggaagtt cgcggtatcg acgatttccg gttcacgcgc tgcattttca
    17461 ataatcgtgg taccttccgc cagggttgca gcacacatga tggtcaccgt tgcgccaacg
    17521 ctgactttat ccatcacgat atgtgcgcct ttcagacgac catcgacgga agctttaacg
    17581 taaccttctt ccagtttgat ggtcgcgcct aattgttcga ggccagaaat gtgtagatca
    17641 accggacgcg caccgatcgt acaaccgcca ggcagtgaaa cttgcccctg accaaagcgc
    17701 gctaccagcg gccccagtgc ccagatagaa gcacgcatgg ttttaaccag atcgtaaggt
    17761 gcgcagaata cattaacgtc gcgggcatca atatgcacag aaccattacg ttctactttc
    17821 gcacccagct ggcttagcag cttcattgat gtatcgacgt ctttcagttt tgggacgttc
    17881 tggatctcta ccggttcttc cgccagtaga gcggcaaaaa ggataggcag agcagcattt
    17941 ttagcgccgg aaattgtgac ttcgccctgg agcttcgttg gcccctgaac acgaaattta
    18001 tccatttagt ttgttctcag ttaacaattc atatccgcta ccggcgaatc gcccatagct
    18061 caaaagccgt tcagtttgcg atcgcgcgcc cactccgcag gggtatacgc tttgatcgac
    18121 acagcatgaa tgcggttatc cgcaatatat tccatcagcg ggccatagac cgtctgctgt
    18181 tttttaaccc gactcatgcc gtcaaacaac tcacccacgg caataacctg aaagtggctg
    18241 ccatcgccgg aaacgtggac ttcctggagg gagagagcgt tcatcaacac gctctgaatt
    18301 tcattatttt ccatgggatc ttcaatcatc agttaataaa ccagcgaaac atcttagagc
    18361 aaagttgcgc tggcataaat aagcaaaaag cctcgctgat aaatcagaca aggctcgact
    18421 tgcaggcagg tttgccggac aggcggttaa cgccatatcc ggcctgaaaa aatttaacga
    18481 ggcagaacat cagcaggcaa attatacaat ttcgcgaggg tatacacttt gtcgtttacc
    18541 ccctgaagcg tcacgttgtt gccctgcttt ttcgccagat cgataagatg gagcagcagt
    18601 gccagtccac ccgtatccac gcgggacaca cggctaagat cgatgcaggt aatccccttc
    18661 accgcttcct cacgcatttc ccaaagcggt agcagaacgt cctgatccag ctctccggat
    18721 aacgccagcg tgtcacccgt ctgcatccag ctcagtgact cgctcattat tttttctctt
    18781 ccagagtgat tttctgttga gaaatcgatt tcagttgcgc agtcaggccg tcgatacctt
    18841 tggtacgcag cagcgttccc cactcgtttt gtttggtggt gatcatactg acgccttcag
    18901 caatcatgtc gtaagcctgc cagtttcccg tctgggagtt tttacgccac tggaagtcca
    18961 gacgcaccgg cggacggcca ttcgggtcaa taatggtaac gcgaataggc acaatggttt
    19021 tatcgcccag cggctgttct ggcgcaatct gataggtttg accgtgatac atcgccagcg
    19081 cctgaccgta agcctgcttc aggtactcac ggaaagcggc aaagtaggct tcacgttgag
    19141 caggggtcgc actcttgtaa tactggccca gcaccagcgc accggcgtat ttcacctgta
    19201 cgtatggcag cagttcctga tcaacaatgg tacgcagata atccgggttg gcccgaattt
    19261 gcggttgctc attcttcagg cgatcgaacg ttttctgcgc cgcctcgtcc atcagcttat
    19321 aaggattggt ctggtctgcc gcggttgccg cactcagagg tgcaatcacc agcaaagcga
    19381 ccatcattaa acgtttaaac atgcgtcggt tctcctgaaa ttatttcgtt gtacccacag
    19441 gttcagtggt ttcattatta cctggcgcag cagctggcgc atcgccacta ttcttattgt
    19501 catcgccttt actaccgtaa aggaactgac caatgagatc ttccagcacc atcgcagact
    19561 tagtgtcctg aattgtatcg ccatccttca ggatagcagt ccccagttcc gggtcttcaa
    19621 aaccgacgtt taatgccaga tattgttccc ccagcaggcc ggaagtacga atgctcagcg
    19681 aactggtatc tggaatgtgg ttataacgtt gttcaatttc cagcgttacg cgcggcagat
    19741 aggttttcgg gtccagcgta atatccgcca cccgacccac aacaacgcca ccaatactga
    19801 ccggagagcg cgctttcagg ccgccaatgt tatcgaacgt cgcataaagc gtgtaggtcg
    19861 gttcagtacg tatggacgtc acgttcgccg ccttcaggca aacaaacagc gccgccagca
    19921 gtgctgctaa taaaaagata cccacccaaa tttcattttt tttcgtttgc atgaactcaa
    19981 ttcccaaaca tcaatgcggt cagcacaaaa tccagcccca gaacagccag agacgagtgg
    20041 acaacggtgc gagtggttgc ccggctaatc ccggcagacg tcgggatggt gtcgtagccg
    20101 ttaaacaacg aaatccacgt caccgtgatg gcgaacacca cgctcttaat cagacagttg
    20161 accagatcca tacgccagtc gacggcattt tgcattgccg accagaagaa cccgctatca
    20221 atgcctttcc aactgacgcc gaccagagat ccgccccaga tccccacggc gacgaaaata
    20281 accgtcaaca gtggtaatga aataacccca gcccagaaac ggggagaaat aacccgacgc
    20341 agcggatcca ccgccatcat ctccatactg gagagttgct ctgtagcgcg catcaggccg
    20401 atttctgcgg ttagcgccga accagcacgc ccggcaaaca acaacgcggc aaccaccggc
    20461 cccagttcac gcagtagcga taacgccacc agcataccca gactggtttc cgcactataa
    20521 gtggtcagaa ccagataacc ttgcagcccc aacaccattc cgatgaacac gccagaaacc
    20581 acaataatca gcatcgacag gacgccgaca ttatagagct ggcgcaccag cagcggcgca
    20641 tgtttgcgaa attccggttt gccgaccagc gcattgaata acattaaccc ggcccgcccg
    20701 aacgttctca gggttttaat ccctttatgt ccgagcgacg ccagcgcatt taacagcatg
    20761 agtggcttaa ctccctggta aaagatcagc gtgataatcg ccggcaggat agcggaacgg
    20821 aacaggcccg tcagctatcc cgtccagaaa ctgacgtacg cgcggatcag gattcgcctg
    20881 caacgcctgg gcactgccat gagcgacaat ttttttgtcc gccaggatcc aggcgtgatc
    20941 cgcaatactt aacacttccg gcacatcgtg agaaaccacc acacaagtca cgcccagcgc
    21001 gctgttcagc tcagaaatca gcttcaccag tacgcccatg gtgatgggat cttgcccaac
    21061 aaaaggttca tcaaacatga tgagatccgg ctccagcgca atcgcacgcg ccagcgctgc
    21121 acgccgcgcc atcccaccgg aaagttcaga aggcattagt ttagccgctc cacgcagccc
    21181 cacggcctcc agcttcatca tcaccgtact atgcaacaat ggcgcgggta gttgggtatg
    21241 ttcgcgcagt ggataggcga cgttgtcaaa tacgttcata tcagtgaaca acgccccgga
    21301 ctgaaataac atgctcatcc gtttgcgcac tgtatacagg cgcgaacgag acatcgccgg
    21361 aatattctca ccatcgaaaa ggatctcacc atgatctggt gcgatttgcc cgccaatcag
    21421 acggagtagc gtcgttttac cgatgcccga tggccccatg atcgccgtga tcttccctcg
    21481 cggcacggtc agggaaatat tatcgaagat gcagcgattg ccacgcgtaa aactgacatc
    21541 gcgcatatcg actaaattcg ccacagactg ctccataatt cacccttcgt cttgcgttga
    21601 ttttctaagc atggcgctca atttaacctt gaacccaaca tatttacaga atattacccg
    21661 ccgtggttag cgaaagctgg catttgtttt actttttagc cgcataaagt caaaattaag
    21721 aatccgttac ggctttctga aaatcttcag cggaccgacg agtatacctg aagaaaggac
    21781 gttagatgct tttagctacg gcactgttaa ttgttggttt acttttggtc gtttacagtg
    21841 ccgaccgcct ggtttttgcc gcgtctattc tttgccgaac ctttggcatc ccgccgctga
    21901 tcatcggcat gacggtggtc agtattggta catcgttacc agaaatcatc gtctcgcttg
    21961 ccgcgtctct gcacgaacaa cgcgatttag ccgttggtac agccctcggc tcaaacatta
    22021 tcaatatttt gctgatcctc ggtctggctg cgctggttcg tccttttacc gtccattccg
    22081 atgttctgcg tcgtgaatta cccttaatgt tgttggtcag cgtagtggcc ggttccgtac
    22141 tctatgacgg acaacttagt cgcagcgatg gtatctttct cctctttctg gctgtgctat
    22201 ggctgctgtt cattgttaaa cttgcacgtc aggctgaacg tcaggggact gacagcctga
    22261 ccagagagca gcttgcagag ctgccgcgtg acggcggatt gcccgtcgcg tttttatggc
    22321 tcggcattgc gcttatcatc atgccagtgg ccacgcggat ggtggttgat aacgccacgg
    22381 tgctggcgaa ttactttgcc atcagcgagt tgacgatggg tctgacggca attgctatcg
    22441 gaaccagcct gccggaactg gcaaccgcaa tagcgggggt tcgcaaaggt gaaaacgaca
    22501 ttgctgtcgg aaatatcatt ggcgcaaaca tttttaatat tgtcatcgtg ttgggtttac
    22561 ccgcgctgat aacgccagga gagattgagc cattggcgta cagtcgtgac tacagcgtga
    22621 tgttgctggt gagcattatt tttgcgttgc tgtgctggcg gcgctccccg caaccgggcc
    22681 gtggtgtagg ggtattatta actggcggat ttatcgtatg gctggcgatg ttgtactggt
    22741 tatcgccaat actcgttgaa taactggaaa cgcattatgt cgcacgtaga gttacaaccg
    22801 ggttttgact ttcagcaagc aggtaaagaa gtcctggcga ttgaacgtga atgcctggcg
    22861 gagcttgatc aatacatcaa tcagaatttc acgcttgcct gtgaaaagat gttctggtgt
    22921 aaagggaaag ttgtcgtcat ggggatgggg aaatcggggc acatcgggcg caaaatggcc
    22981 gcaacgtttg ccagcaccgg tacaccttca tttttcgtcc atcctggtga agccgcgcat
    23041 ggtgatttag gcatggtcac cccacaggat gtggtgattg ctatctctaa ctcaggtgaa
    23101 tccagcgaaa tcacggcctt aattccagtg cttaagcgtc ttcacgtacc gttaatctgc
    23161 atcaccggtc gcccggagag cagcatggcg cgcgccgcag atgtgcatct gtgtgttaaa
    23221 gtagcgaaag aagcctgtcc gttagggctg gcaccgacca gcagcaccac cgccacgctg
    23281 gttatgggcg atgccctcgc tgtcgcgctg ttaaaagcac gcggctttac tgctgaagat
    23341 tttgcgctct cacacccagg cggcgcactg ggtcgtaaac ttctgctgcg cgtaaacgat
    23401 attatgcata cgggcgatga gatcccgcat gttaagaaaa cggccagtct gcgtgacgcg
    23461 ttgctggaag ttacccgcaa aaatcttggt atgactgtca tttgcgatga caatatgatg
    23521 attgaaggca tctttaccga cggtgattta cgccgtgtct tcgatatggg cgtggatgtt
    23581 cgtcagttaa gtattgccga tgtgatgacg ccggggggaa tacgtgtgcg ccctggcatt
    23641 ctggccgttg aggcactgaa cttaatgcag tcccgccata tcacctccgt gatggttgcc
    23701 gatggcgacc atttactcgg agtgttacat atgcatgatt tactgcgtgc aggcgtagtg
    23761 taaagattca aggataaaca acaatgagca aagcaggtgc gtcgcttgcg acctgttacg
    23821 gccctgtcag cgccgacgtt atagcaaaag cagagaacat tcgtctgctg atcctcgatg
    23881 tcgatggcgt actgtcagat ggcctgattt atatgggcaa taatggcgaa gagctgaaag
    23941 cgttcaatgt tcgtgacggt tatggcattc gttgtgcgct cacctctgat attgaagtcg
    24001 ctatcattac cgggcgaaag gctaaactgg tagaagatcg ttgtgccaca ttggggatca
    24061 ctcacttgta tcaggggcag tcaaacaaac tgatcgcctt tagcgatctg ctggaaaaac
    24121 tggcgattgc cccggaaaat gtggcttatg tcggcgatga tctcatcgac tggccggtaa
    24181 tggaaaaagt gggtttaagc gtcgccgtgg ccgatgcgca tccactgttg atcccgcgcg
    24241 ccgattacgt gacgcgcatt gctggcggtc gtggcgcagt gcgcgaagtt tgcgacttat
    24301 tactcctggc gcagggcaaa ctggatgaag ccaaagggca atcgatatga gtaaagccag
    24361 acgttgggtt atcattgtgc tatcactggc ggttctggtg atgatcggca ttgatatggc
    24421 cgaaaaagac gataccgccc aggtggtcgt caacaacaat gatcccacct ataaaagcga
    24481 gcatacggac acgctcgtct ataacccaga aggggcacta agctatcgat tgattgctca
    24541 acacgttgaa tattattccg atcaggccgt ttcgtggttt acgcagccgg tacttaccac
    24601 gtttgataag gataaaatcc cgacatggtc cgtaaaagca gataaagcca agctgaccaa
    24661 tgaccggatg ctctatttat atggacacgt tgaagtcaac gcacttgtgc caaactctca
    24721 acttcgcaga atcacgacgg ataacgcgca gatcaatctg gtgacgcagg atgttacctc
    24781 tgaagacctc gtcacgttat acggaacaac atttaactcc agcggtctga aaatgcgcgg
    24841 caacttacgc agcaagaacg ccgagctgat tgaaaaggtt agaacatcct atgaaattca
    24901 aaacaaacaa actcagcctt aatcttgtgc ttgccagctc acttctggcc gccagcattc
    24961 cggcatttgc cgtaaccgga gacactgatc agccgatcca cattgaatcg gaccagcaat
    25021 ctcttgatat gcaaggcaac gtggttacct ttaccggtaa tgtcatcgtc acccagggca
    25081 ccatcaaaat taatgccgac aaagtggtcg ttacccgtcc gggcggcgaa caaggtaaag
    25141 aagtgattga cggctacggt aaaccggcaa cgttctacca gatgcaggac aacggtaaac
    25201 ccgttgaagg tcacgcttcc cagatgcact acgaactggc aaaagatttt gtcgttctga
    25261 cgggtaatgc ttatctgcag caggtcgata gcaacattaa gggcgataag atcacttacc
    25321 tggtaaaaga gcagaaaatg caggctttca gcgacaaagg caagcgcgta accaccgttc
    25381 tggtgccgtc gcagctgcag gacaaaaaca acaaaggcca gaccccggca cagaagaagg
    25441 gtaattaatt cgttatggca acattaactg caaagaacct tgcaaaagcc tataaaggcc
    25501 gtcgcgtggt agaagacgtc agcctgaccg tcaactccgg ggaaattgtc ggtctgctgg
    25561 ggccaaacgg tgccggtaag accaccactt tctacatggt tgtaggcatt gtgccgcgcg
    25621 atgcgggcaa catcattatt gatgatgacg atatcagtct gctgcctctg catgcacgcg
    25681 cgcgccgcgg tatcggctat ctgccacagg aagcctccat tttccgtcgc ctcagcgttt
    25741 acgataacct gatggcggta ctgcaaattc gtgacgactt gtctgctgaa caacgtgaag
    25801 accgcgcgaa cgagctgatg gaagagtttc acattgagca cctgcgtgac agcatggggc
    25861 agtcactctc cgggggtgaa cgtcgccgtg tagaaattgc ccgcgcactg gctgcgaatc
    25921 cgaaatttat tctgctcgac gaaccgtttg ccggggttga cccgatctcg gttatcgaca
    25981 ttaaacgcat cattgagcac ctgcgcgaca gcggcctggg cgtgctgatc actgaccaca
    26041 acgtgcgtga aacactggcg gtttgtgaac gcgcttatat cgtcagtcag gggcatttga
    26101 tcgcccacgg cacgcctaca gaaatcttac aagacgaaca cgttaagcgt gtataccttg
    26161 gggaagactt cagactctga tagggtagaa gtttgcgacg ttttagcagg agagtacgat
    26221 tctgaacatg aagcaaggtt tgcaactcag gcttagccaa caactggcga tgacgccaca
    26281 gctccaacag gcaattcgtc tgttgcagtt gtcgacgctg gaacttcagc aggagctaca
    26341 gcaggcgctg gagagtaatc cgctgcttga gcaaatcgac actcatgaag aaatcgacac
    26401 ccgcgaaacg caagacagtg aaacgctgga caccgccgac gcgctcgaac aaaaagagat
    26461 gccggaagag ctgccgctcg atgccagttg ggacaccatt tacaccgctg gtacaccatc
    26521 cggcaccagc ggtgactaca ttgacgatga gctgccggtc tatcagggcg aaacgacgca
    26581 gaccttgcag gattacctga tgtggcaggt cgagctgaca ccgttttccg acactgaccg
    26641 cgctattgct acctctatcg tcgatgccgt tgatgacatc ggttatctga ctgtcccgct
    26701 ggaagatatt ctcgaaagta tgggcgatga agagatcgac atcgacgagg ttgaagccgt
    26761 ccttaagcgg atccaacggt ttgatccggt cggtgtagcg gcaaaagatc tgcgtgactg
    26821 cctgctaatc caactctccc aattcgataa aaccacgccg tggctggaag aggccagact
    26881 gatcattagc gatcatctcg atctgttagc caatcacgac ttccgcactt taatgcgcgt
    26941 cacgcgtctg aaagaagatg tgctgaaaga agccgtcaat ctgatccagt cgctcgatcc
    27001 gcgccccggg cagtcgatcc agactggcga acctgagtat gtcattccag atgtgctggt
    27061 gcgtaagcat aacggtcact ggacggtaga actcaacagt gacagcattc cgcgtctgca
    27121 aatcaaccag cactacgcct cgatgtgcaa taacgcgcgc aacgatggtg acagccagtt
    27181 tatccgcagc aatctgcagg atgccaaatg gttgatcaag agtctggaaa gccgtaacga
    27241 tacgctactg cgcgtgagtc gctgtatcgt tgaacagcag caagccttct ttgagcaagg
    27301 tgaagaatat atgaaaccga tggtactggc cgatatcgcc caggctgtcg aaatgcatga
    27361 atcgacgata tctcgcgtga ccacgcaaaa atacctgcat agtccacgag gcatttttga
    27421 actgaagtat ttcttttcca gtcacatcaa taccgagggc ggcggcgaag cttcctccac
    27481 ggcgattcgt gcgctggtga agaaattaat cgcggcggaa aacccagcga aaccgttgag
    27541 cgacagcaag ttaacctctt tgctgtcgga acaaggtatc atggtggcac gccgcactgt
    27601 tgcgaagtac cgagagtctt tatccattcc gccgtcaaac cagcgtaaac agctcgtttg
    27661 acccaaccga taaggaagac actatgcagc tcaacattac cggaaataac gtcgagatca
    27721 ccgaggcact gcgcgaattt gttacagcca aatttgccaa acttgagcaa tattttgacc
    27781 gaatcaacca ggtctatgtt gttctgaaag tggagaaagt cacccacacc tcagatgcaa
    27841 cactgcatgt aaacggtggt gaaattcatg ccagcgcgga aggtcaggac atgtacgctg
    27901 ccattgatgg cttaattgat aagctggcac gccagttgac caaacataaa gataaactga
    27961 aacaacacta attgtccggg caattagcat gtgcatggcg gcctgttgtg cggcacaacg
    28021 ggccatttgt acggttaatg ctccgagcct gttccactgt ttgagtgggc aggttcttag
    28081 gtgaaattat gacaaataat gatacaactc tacagcttag cagtgttctt aacagggaat
    28141 gtacgcgaag ccgcgtccac tgtcagagca aaaaacgcgc cctggaaatc atcagcgagc
    28201 tggcggcgaa acaacttagc ctgccgcctc aggtggtttt tgaagctatc ctgacgcggg
    28261 aaaaaatggg cagtaccggt atcggcaatg gtattgccat tccgcatggc aaactggaag
    28321 aagatactct gcgcgccgtt ggcgttttcg ttcagctcga aacgcctata gctttcgatg
    28381 ccatcgacaa ccaaccggtg gacttacttt ttgccctgct ggtgtcggca gaccaaacta
    28441 aaacgcacct gcatactctg tcgctggtgg cgaaacgtct ggcggacaaa accatctgcc
    28501 gccgtttacg cgcagcccag agcgatgaag agctgtatca aatcattacg gataccgaag
    28561 gtactccgga tgaagcgtag ttattcggta atgtctcttt tagacgttgt gaggagaaac
    28621 agtacatggt actgatgatc gtcagcggac gttcaggttc aggtaaatct gtcgccctgc
    28681 gtgcgctgga agatatgggt ttttactgcg tggataacct tcccgtagtg ttgttacccg
    28741 atctggctcg aaccctggcc gatcgcgaga tttctgccgc cgtcagcatt gatgttcgta
    28801 atatgccgga gtcaccagaa atattcgaac aggcgatgag taacctgcct gacgctttct
    28861 caccgcaact cctgttcctg gatgccgacc gtaatacctt aattcgtcgt tacagtgaca
    28921 cgcgccgact gcatccgctt tccagcaaaa acctgtcgct ggaaagtgct atcgacaaag
    28981 aaagcgattt gctggagcct ctgcgttcgc gagcggatct gattgtcgat acctcagaaa
    29041 tgtccgttca cgagctggct gaaatgctgc gtacccgtct gctgggtaaa cgtgaacgcg
    29101 aactgaccat ggtctttgag tctttcggct tcaaacacgg tatccctatc gatgcagatt
    29161 acgtctttga cgtgcgcttc ttgccgaacc cgcactggga tccgaaactg cgtccaatga
    29221 caggtcttga taaacctgtc gccgcgttcc tcgaccgcca cacagaagta cacaatttta
    29281 tctaccagac gcgaagctat cttgagctat ggttacctat gctggaaacc aacaaccgta
    29341 gctacttgac ggtcgccatt ggttgtaccg gcgggaagca ccgttcggtg tatattgcag
    29401 agcaactggc agactacttc cgctcgcgcg gtaaaaacgt ccagtcacgc catcgtacgc
    29461 tggaaaaacg taaaccatga ccgtcaagca aactgttgaa atcacaaaca agctgggcat
    29521 gcatgcccgg cctgcaatga agctgtttga attaatgcag ggttttgacg ctgaagtgct
    29581 gttacgtaat gacgaaggca ccgaggccga agccaacagc gttattgcgc tgctgatgtt
    29641 ggattctgcc aaaggacggc agattgaagt tgaagcgacc ggcccacagg aagaggaagc
    29701 actggccgcc gttatcgccc tctttaactc tggttttgat gaagattaat cttcatcact
    29761 ttgacataca actcccttca aactcccccc gacaataaga aaatcacgta ctgaaatcgt
    29821 tctcaatcaa cgtcatttgt acattttgtg cgcttttcac tttcagaaga accttaagaa
    29881 aaccttaaga ggcattgttt aggttttgtt taagttaatc gaccatactg gagatcgtca
    29941 gaaaatattt ccaggagatg gcatgattcg cttatctgaa caaagtcccc tgggcaccgg
    30001 aagacatcgg aaatgttatg cgcatccgga agatgcccaa cgctgtatca agattgtcta
    30061 ccatcgtggc gatggtggcg ataaagagat ccgccgtgag ttaaagtact acgcgcatct
    30121 tggtcgccgg ttaaaagact ggagtggaat accgcgctat cacggtaccg ttgagacgga
    30181 ttgcggaacc ggttatgtct acgatgtgat tgcagatttt gacggcaaac tctccattac
    30241 gctgaccgaa tttgctgaac aatgccgtta cgaagaagac attgcacaac tgcgccagct
    30301 actgaaacag ctgaagcgtt atttgcagga taaccgtatc gtgacgatgt cgctgaagcc
    30361 gcagaatatc ctctgtcacc gcatcagtga gtcagaggtt atcccggtcg tctgcgacaa
    30421 cattggtgaa agcacattga ttccactggc gacctggtcg aagtggtgtt gtctgcgtaa
    30481 gcaagagcgg ttatggaaac gatttatcgc ccaaccggcg ctggctattg ccctgcagaa
    30541 agatctgcaa cctcgtgaga gcaaaacgct ggccctcact tcgcgcgaag cttaatccag
    30601 ctggtgttgt tgcataaacg gctcaccgcc taactgatac atctgccgta aaatccacgc
    30661 ctgacggcta cgcacgtagc ccgatggcga ggagactttg aaacgaagtg gattaggtaa
    30721 tacagctgcc agtaatgcag cttccgaccg ggtaagtttg ctcgcgggtt tgtggaaata
    30781 acgttgtgcc gcagcttcga cgccaaacac accgtcgcca aattcggcga tattcaggta
    30841 aaccgtcagg atacgctttt tgctccagac tgtttctatc cccagcgtta atccagcttc
    30901 cagccctttt cgtacccagc tacgcccatc ccataaaaag agatttttgg ccgtctgctg
    30961 agaaatcgtt gaagcaccgc gaatacggtt ttcattgcgc tcgttgtgcg ccagggcttt
    31021 ctcaatggaa gcgacatcaa agccccagtg ctcagaaaat ttctgatctt ctgcggcaat
    31081 caccgccagt cccatccacg gcgagatttg atccatactg acccagtcag aatgtgccac
    31141 gtaacgaaaa ttgccatgca gccaggcgct gacctgtcgc tcgaccatta ccgctgagaa
    31201 gggaacaggc gcaacactaa acaacgcgat gcccccgccc cagaaaacgg cgaggacaac
    31261 cattaacagc aaaagaaaac ggcgaacgaa actaaacacc gttaagcggc ttttactcat
    31321 tcagccagaa ccagcacgcg ggaaaccagc ttatcaatgc cgctcgccgc ttctgcaatg
    31381 ttctgcgcca gcatatatgc tggggtggtg acaatcttat tgtcttcatc aaccacgata
    31441 tcatcgacag gacagggcac atgctccgcg cccatctctt ccagcacttc tgcggtatcg
    31501 atatcagtac cgatggtcaa acgcagcggg aaatcgaaaa ttttcggcag catcgccggg
    31561 gcaatacaca taaaaccaag cggttttccg gcctgatgca tcgcttgtgc cagcgccttt
    31621 aattcacggt caacggtgca ttcgctacca agagtggcaa aattgcttaa attcttcgcc
    31681 gcgccaaacc cccccggcac aatcaacgca tccagttcag cggcatcggc ctgggccaga
    31741 ggacggattt caccgcgcgt tattcgtgcc gcttcaatca gcacgttgcg cgtttccgtc
    31801 atcgcttcgc cagttaaatg gttgataaca tcaacctgct gcttatccgg tgcaaagcag
    31861 accgcctgcg caccgctgcg tgaaatagct aacaacgtca acaccgcttc atgaatttca
    31921 gaaccgtcat agacgccgca tccgctcaga attacgccaa ttttcttcat tgtgatcatc
    31981 ctttcgcaat tgactgaaac acattaattt ttttaataaa aatggtacgc atcacacatt
    32041 taactgattc atgtaacaaa tcatttaagt tttgctatct taactgcgtg cggcctgaaa
    32101 aacagtgctg tgcccttgta actcatcata ataatttacg gcgcagccaa gatttccctg
    32161 gtgttggcgc agtattcgcg caccccggtc tagccggggt cattttttag tggcttttgc
    32221 cacccacgct ttcagcactt ctacgtcgtg acgccactct tctttcatct cttcaatcca
    32281 ttcaccgacg ttatcttccc aggcaggaag gtcaggagac tgaatttgct gacccagctg
    32341 ttgcagatgg cgtaacccca ctgaccccgc cgcaccttta attttatgtc cttcctcaac
    32401 aatgcctttt ttatcctgcg ccgtcagatt cgactccagc acgctgacat agcccggcat
    32461 catcttctca aacaccgcta acccgtcggt gatcagcttc ggtcctacaa gttcgagata
    32521 ctgttccagc atgggaatat cgagcaatgc ttctgattta ctgttctctt ctgtcgtcac
    32581 cgtactctcc tcatcatcct gggtatccca gaatttcttg atcatcgcgg ttagcgccgg
    32641 aacagaaagc ggcttactca gcacatcatc cattccagca ttgaggtact cttgtttgtc
    32701 tttcagcacg ttagcggtta aggccaccag cggcggtaaa tcctctcgcg gataacgctt
    32761 cgtcagttca cgagagatat ccagcccggt catatctggc aactgaatat ccagtaacac
    32821 caggtcgtat tcgcccggtt taaacatctc tagcgccgcc ttgccggtca tggcgacatc
    32881 aacgctgtta cctaattttt ccagcacaga acgcgcaaca atcacgttca gttcaatgtc
    32941 ttccaccagc agcacattca gcgccggtaa aggcatatcg tcttcatcaa acgcatcatc
    33001 gacctcttct gctaccgacg gtgcgtggat cgtcaacgta aaggttgaac ctttgccctg
    33061 ttcgctggta accgtaatat cgccgcccat atttttcgcc agacgacgag aaacggccag
    33121 accaataccg gtgccggtgg caggtttacc gccatgactg tctttcacct ggtaatacat
    33181 ggcgaaaatt ttatccagct catcctgcgg aatgccgata ccagagtctt ccacttcaaa
    33241 atgcagcata tcgccttcat cgtagcgcac gcgcacggta acctggcctt gctgggtgaa
    33301 tttgacggcg ttactgatga ggttccacag gatctgccgt aaacgcgtcc cgtcggtaat
    33361 gacctgatgc ggtaatggca gcgtcggctc caggttaaag cgcaatcctt tttgttgcgc
    33421 ctgcaatgcg gagagatttt ccagatcggc aaggaagctg gtgaaatcaa ccggttgatt
    33481 atcaagctgg accttgcgcc gttccatctt atccatgtcg ataatatcgt taaagatatt
    33541 ccccagcgtg acggccgaaa catggatggt cttgagatat ttttcctgct cggcggtgag
    33601 ttcggtatcc agcagaatgc ggctcagacc gacgataccg ttcagcggtg tacgcaattc
    33661 gtgactgatg gtggagataa acgtcgtttt gtcgcggctg gcccgttcaa gcgcatcctg
    33721 ataccgctta cgctcggtaa tgtctcgacc aaagcccatc aaaccgtgac gtttacccac
    33781 gcggtcgtag tacggcactt tacggatttc aaagcaggct ttgcgcccgt ccgggtaatc
    33841 cagccactgt tcataggtca gtgacacatt atgacggaac actttttcat cggtttcaat
    33901 gacttttgcg gcggcttccg gtgagtaaac atcaacaggt ttcaggtgaa ccagttgttt
    33961 ttcgcttttt ccggtcagca gctccatcgc gcggttacag ccggaaaact ctttatcttc
    34021 gttacgataa aaaaccaggt cgggtgaagc atcaaggaag gaacgtaaga atgaggattg
    34081 ctgctcgagc tgaatttgtg tctcttcgcg ctctttgatt tcaattttca gttggccgaa
    34141 ggtttcctgc agttctgctt ccgctttttc acgaacggcg atttcctgat ttagctgggc
    34201 aatattatct tttaactgaa cgttgaggct caaatcgcgc tcgcgcatct cctccagttt
    34261 ttgcaccagc cgtgacagac gttgtcgtga ctcctccagt tgctcgacga ccaccgatag
    34321 aaagtagacc gcccacggcg taatcaacaa accaaagaag atagaacgaa taacatcaat
    34381 gctttcgacc tgaccatgca gcaccatggt taccgccatt tgtaccacaa tggcaagaac
    34441 gacgagggcc agcgccagca acattgagaa gcgcaccaga cctaacttca tcatcaggtc
    34501 aacataatac tgcgccagca gacgaatttg cttcattagg gaattccttc acgacaacct
    34561 gccccaataa tacccaattc tgacagttac gttataaatt gtgtgagaaa tgcggaattc
    34621 ccctcaacga cctactccgt tggaggtagc cacggacgtc ccagcgccga tccctgtacg
    34681 ccatgttcat tcagatagcg atccagctcg accattcccg tccatcgatt ttcgcaccac
    34741 agaggagcaa gcagcgtcgg gcgacgggcg ctggcggaaa tgcggtggta gatcacttcc
    34801 ggtggcgtat ggcgaatcat ttctccggca gtgagcgtgt aatcctccag ttcaataccg
    34861 ttcaaacgcc ccgcttccca ggctttcgcc ataatgctgc ctttcacaat atgcaacgga
    34921 tgtagcttta taccgtcaac accggtttcc actacccgct ccagcgtttg cagacattca
    34981 gccagccctt cgcctggcag accgacaatt aaatgcgagc acaccttcag cccgcgctgg
    35041 cgtgccagct gcgtcgtgcg ctgataacag gcgaaatcat gaccgcgatt aatgcgatgt
    35101 agcgttttgt cgtgcacggt ttgcagcccc agctccagcc acacttcgta gccctggtct
    35161 ttatattcgc aaagcagatc cagcaccgca tccggcacgc agtccgggcg cgtgccgaca
    35221 cacaaaccga caatattggc ctggctcacc gcctgttgat acatcgaacg cagcacctga
    35281 acttcggcaa aggtgctggt atacgcctgg aaataggcga ggtaacgttt cgcgcgatta
    35341 attaaattcg cctgatgcgc cagctgttcg gcaatggaac gatgctgctg cgcttcgtcg
    35401 gcaaacgagg caacattaca gaaggtgcag ccgccgcgcc cgatggtgcc gtcgcgattc
    35461 gggcaactga aaccgccgtg cagcgtcagc ttatgcacct tttgcccata acgacgggtg
    35521 agatcaccac caaacatatt gactaatttc tgtaactgca taatctgata gaccgcgcct
    35581 tgaaaagagg ccaaagcctg ccatttttag ctcaattcgg cgatgacctg gatcaatcgt
    35641 cccggcccgc ttttatcaac tgcataatca atcaaaatta ccgaaatttc atgcataatc
    35701 acataaatca cttttgctta tcttgtgtca gattttttta tctccttatg gattttaggc
    35761 aaaaacagta gcatgaaacg tcattaccaa ttaatgcagt ataaaatgct ggttttctcg
    35821 tcaattcaag gcaggataag ggttaacacg cctttatgac agtcaggaat tgactgtttc
    35881 tctaacgact tcccttttag ccttaaagat aaaatctatt ttaatttcag acatttaata
    35941 aagaattttg cgctaaagca catttctgta ccaataagct tgccatttga cctgtatcag
    36001 ctttcccgat aagttggaaa tccgctggaa gctttctgga tgagcagcct gctcatcata
    36061 tttatgcagt aattgagatc ccctcttcac cgtattaacc gatgcgaaaa ggacaacaag
    36121 ggggcgaatg cgaggcgcgc gtatgacacg caacccccgt cgccacgctc tttctgtacc
    36181 cgtgcgcagc ggttcggtag tggggttccc gcagagcctg ggggaggttc acgatatgtt
    36241 gtacgataaa tcccttgaga gggataactg tggtttcggc ctgatcgccc acatagaagg
    36301 cgaacctagc cacaaggtag tgcgtactgc aatacacgca ctggcccgca tgcagcaccg
    36361 tggcgcgatt ctcgccgatg gtaaaaccgg tgacggttgc ggcttgctgt tacaaaaacc
    36421 ggatcgcttt tttcgcatcg ttgcgcagga gcgcggctgg cgtttagcga aaaactacgc
    36481 cgtcgggatg ctattcctga ataaagatcc tgaactcgcc gctgccgcac gccgcatcgt
    36541 tgaagaagaa ctgcaacgcg aaaccttgtc gattgtgggc tggcgtgatg tccccactaa
    36601 cgaaggcgta ctgggtgaaa tcgccctctc ctctctgccc cgcattgagc aaatttttgt
    36661 gaacgccccg gcaggctggc gtccgcgcga tatggagcgc cgtctgttta tcgcccgccg
    36721 ccgcattgaa aagcgtctcg aagccgacaa agacttctac gtctgtagcc tatcgaatct
    36781 ggtgaacatc tataaaggtc tgtgtatgcc ggcggatctg ccgcgctttt atctggatct
    36841 tgcggacctg cgtctggaat cggccatttg cctgttccac cagcgcttct ccactaacac
    36901 cgtaccgcgc tggccgctgg cgcaaccgtt ccgctatctg gcgcataacg gtgaaatcaa
    36961 caccatcact ggtaaccgcc aatgggcgcg tgcgcgtacc tataaattcc agacgccgct
    37021 tatccctgat ctgcacgacg ccgcaccgtt cgtcaacgaa accggctctg actccagttc
    37081 gatggataac atgctggaac tcctgctggc aggcgggatg gatatcatcc gcgccatgcg
    37141 tctgttagtg ccacccgcct ggcagaacaa cccggatatg gacccggaac tgcgtgcctt
    37201 ctttgacttt aactccatgc atatggagcc gtgggatggc ccggcgggca tcgtgatgtc
    37261 cgacggtcgt tttgccgcct gtaacctcga ccgtaacggt ctgcgtccgg cgcgctacgt
    37321 catcaccaaa gataagctca tcacctgcgc ctctgaagtc ggtatctggg attaccagcc
    37381 tgacgaagtg gtcgaaaaag gccgcgtcgg gccaggcgaa ctgatggtta tcgacacccg
    37441 cagtgggcat attctgcact cggcagaaac cgatgacgat ctgaaaagcc gccatccata
    37501 taaagagtgg atggagaaaa acgtccgccg actggtaccg tttgaagatc tgcccgatga
    37561 agaagtgggt agccgcgaac tggacgacga cacgcttgcc agctaccaga aacagtttaa
    37621 ctacagcgcg gaagagctgg actccgtaat tcgcgtactg ggcgaaaacg gtcaggaagc
    37681 ggtcggttcg atgggcgatg ataccccatt cgccgtgctc tccagtcagc cgcgcattat
    37741 ttacgactac ttccgccagc agtttgccca ggtgactaac ccgccaatcg acccgctgcg
    37801 tgaagcgcat gttatgtcgc tcgccaccag tatcggtcgt gaaatgaacg tcttttgcga
    37861 agcagagggc caggcgcacc gtttaagctt taaatcgccg attctgctct actccgattt
    37921 caaacagctc acgacgatga aagaggagca ctaccgcgca gatacgctgg atatcacctt
    37981 tgacgtcact aaaaccacgc tcgaagcgac agtcaaagag ctgtgcgaca aagccgaaaa
    38041 aatggtacgt agcggcaccg tgctgctggt gctctccgac cggaatatcg ctaaagatcg
    38101 cctgccggtt ccagccccga tggcggttgg cgcgatccag acccgtctgg tcgatcaaag
    38161 cctgcgttgc gatgccaaca tcatcgtcga aaccgccagc gcccgcgatc cgcaccactt
    38221 cgccgtgttg ctgggcttcg gcgcgacggc tatttatcca taccttgcct atgaaacgct
    38281 gggccgcctg gtagacaccc atgcgattgc caaagattat cgtaccgtga tgctcaacta
    38341 ccgtaacggc atcaacaaag gcttgtacaa aatcatgtcc aaaatgggca tctccaccat
    38401 cgcctcttac cgctgctcga aactgtttga agcggtcggt ctacacgatg atgtagtggg
    38461 cctgtgcttc cagggggcgg tcagccgcat tggtggggca agctttgaag acttccagca
    38521 ggatctgctg aatctgtcga aacgtgcctg gctggcgcgt aagcccatca gccagggcgg
    38581 cctgctgaaa tacgtccacg gcggcgaata ccacgcctac aacccggacg tggtgcgcac
    38641 gctgcaacaa gcggtacaaa gcggcgagta cagcgactat caggaatacg cgaagctggt
    38701 taatgagcgt ccggcaacca cgctgcgcga tctgctggca attacgccgg gtgaaaacgc
    38761 ggtcaacatt gctgatgttg aaccggcaag cgaactgttt aaacgcttcg ataccgctgc
    38821 gatgtctatc ggcgcgttaa gcccggaagc ccacgaggcg ctggcggaag cgatgaacag
    38881 catcggcggt aactcaaact ccggtgaagg cggtgaagat ccggcgcgct acggcaccaa
    38941 caaagtgtcg cgcatcaagc aggtggcttc cggtcgcttt ggggttactc cggcgtatct
    39001 ggtcaatgcc gacgtcattc agattaaagt cgcccagggc gcgaagccgg gcgaaggcgg
    39061 tcagttgccg ggtgataaag tcactcctta catcgccaaa ctgcgctatt cggtgcccgg
    39121 agtgacgctg atctccccgc cgccgcacca cgatatctac tctatcgagg acttagcgca
    39181 gctcattttc gacctcaagc aggttaaccc gaaagcgatg atctccgtga agctggtttc
    39241 cgaaccgggc gtaggcacca tcgcgactgg cgtggcaaaa gcttatgccg atttaatcac
    39301 catcgcaggc tatgacggtg gcaccggcgc aagcccgctg tcgtcagtga aatacgcagg
    39361 ttgcccgtgg gagctggggc ttgttgaaac ccagcaggcg ctggttgcca atggtctgcg
    39421 tcataagatc cgtttgcagg tcgatggcgg cctgaaaacc ggtgttgata tcatcaaagc
    39481 ggcgattctc ggcgcagaaa gcttcggctt cggcactggc ccaatggtcg cgctcggctg
    39541 taaatatctg cgtatttgcc acctgaacaa ctgcgcaacg ggtgtggcaa ctcaggatga
    39601 caaactgcgt aagaatcact atcacggcct gccgttcaag gtgacgaatt actttgagtt
    39661 tatcgcccgt gaaacccgcg agctgatggc acagctgggg gtaacgcggc tggtggatct
    39721 gattggtcgc accgacctgc tgaaagagct ggacggtttc accgccaaac agcagaaact
    39781 ggcgctgtcg aagctgctgg agaccgccga accgcatcca ggtaaggcac tctactgcac
    39841 cgaaaacaac ccgccgtttg ataacggcct gctgaacgcg cagttgctgc aacaggcgaa
    39901 accgtttgtc gatgaacgcc agagcaaaac cttctggttc gatatccgca ataccgatcg
    39961 ctccgtgggc gcgtcgcttt caggctatat cgcccagacg cacggcgatc aggggctggc
    40021 agccgatcct atcaaagcgt acttcaacgg caccgcaggc cagagtttcg gtgtatggaa
    40081 cgcgggcggc gtggaactgt acctgaccgg ggatgccaac gactatgtcg gtaaaggcat
    40141 ggcgggcggc ttaatcgcca ttcgtcctcc ggttggttcc gccttccgca gccatgaagc
    40201 aagtattatc ggcaacacct gcctgtatgg cgcgaccggt ggtcgtctgt atgccgcagg
    40261 ccgcgcgggt gaacgtttcg gcgtgcggaa ctccggtgct atcaccgtgg tagaaggcat
    40321 tggcgacaac ggctgtgaat atatgacggg cggtattgtc tgtattctgg gtaaaaccgg
    40381 cgttaacttc ggtgcgggca tgaccggcgg cttcgcttac gttctcgatg aaagcggtga
    40441 tttccgcaaa cgcgttaacc cggaactggt cgaggtctta agcgttgacg atctggcgat
    40501 ccatgaagag catctgcgcg gtcttatcac cgagcatgtt cagcatactg gctctcagcg
    40561 cggtgaagag attctggcga actggtcaac cttcgccact aaatttgcgc tggttaaacc
    40621 gaagtccagt gatgtaaaag cactgctggg tcatcgtagt cgtagcgcag cagagttgcg
    40681 cgtgcaggcg cagtaagggg tagcaacaat gagtcagaat gtttatcaat ttatcgacct
    40741 gcagcgcgtt gatccgccaa agaaaccgct gaagatccgc aaaattgagt ttgttgaaat
    40801 ttacgagccg ttttccgaag gccaggccaa agcgcaggct gaccgctgcc tgtcgtgcgg
    40861 caacccatac tgcgagtgga aatgtccggt acacaactac atcccgaact ggctgaagct
    40921 cgccaacgag gggcgtattt ttgaagcggc ggaactgtcg caccagacca acaccctgcc
    40981 ggaagtttgc ggacgagtct gcccgcaaga ccgtctgtgc gaaggttcct gcactctgaa
    41041 cgatgagttt ggcgcggtga ccatcggcaa cattgagcgt tatatcaatg ataaagcgtt
    41101 cgaaatgggc tggcgtccgg atatgtccgg cgtgaagcaa accggtaaaa aagtggcgat
    41161 tatcggcgca ggcccggcag gtctggcgtg tgcggatgtc ctgacgcgta acggcgtaaa
    41221 agccgttgtc ttcgaccgtc atccagaaat tggcgggctg ctgaccttcg gtattccggc
    41281 cttcaagctg gaaaaagagg taatgacgcg tcgccgtgaa atcttcaccg gcatgggtat
    41341 tgaattcaaa ctcaataccg aagtgggccg cgacgtgcag ctggacgatc tgctgagtga
    41401 ttacgatgcc gtcttccttg gcgtcgggac ttatcagtca atgcgcggcg ggctggaaaa
    41461 cgaagacgcc gatggcgtgt acgcagcgct gccgttcctt atcgccaaca ccaaacagtt
    41521 aatgggcttt ggtgaaaccc gcgacgaacc gttcgtcagc atggaaggca aacgcgtggt
    41581 ggtccttggc ggtggcgaca ctgcgatgga ctgcgtgcgt acgtccgtgc gccagggagc
    41641 gaagcacgtt acctgtgcct atcgtcgtga tgaagagaac atgccgggtt cccgccgcga
    41701 agtgaaaaac gcgcgggaag aaggcgtaga gttcaaattc aacgtccagc cactggggat
    41761 tgaagtgaac ggtaacggca aagtcagcgg cgtaaaaatg gtacgtaccg aaatgggcga
    41821 accggatgcc aaaggccgtc gccgcgcgga gatcgttgca ggttccgaac atatcgttcc
    41881 ggcagatgca gtgatcatgg cgtttggttt ccgtccacac aacatggaat ggctggcaaa
    41941 acacagcgtc gagctggatt cacaaggccg catcatcgcc ccggaaggca gcgacaacgc
    42001 cttccagacc agcaacccga aaatctttgc tggcggcgat atcgtccgtg gttccgatct
    42061 ggtggtaacc gctattgccg aaggtcgtaa ggcggcagac ggtattatga actggctgga
    42121 agtttaagct ctgtcccagc gccgcctcct cagaaaagag tcggcgctga aattctcctt
    42181 tctttctgga aagcgcctcg cataatctga agaaaaccaa tgtaatacgc tcatctggat
    42241 tgttaccggt agatgcccgg cgtatagctg attccattat tcaacgcagc gccatataaa
    42301 gggacagata atggaatcgc tctcagagag aacctcaaca ggctaccagc aaatccacga
    42361 cggtattatt cacctggtcg atagcgcccg gacggaaacg gtacgtagcg ttaacgcgct
    42421 gatgaccgcg acatactggg aaattggccg acgaattgtc gaatttgaac aaggtggcga
    42481 ggcaagggct gcgtatggtg cgcagctaat caagcgacta tcaaaggatt taagtctaag
    42541 gtataagcgt ggtttctctg caaaaaactt acgccaaatg aggctttttt acctcttttt
    42601 tcaacatgtt gaaattcgcc agacagtgtc tggcgaatta acaccattgg gaattccgca
    42661 gacaccatct gcagaattcc catcggcaaa gatttggcag acgctgtctg ccaaattgag
    42721 aggctgtttg atatttccac agcttttgta ttgcatctcc tattttcggt ttgtatattc
    42781 atgcaaataa acgaaggaaa gttgccgtga tttacattga acagcttgaa ttaatccata
    42841 aaagtggtga tgtgttgtat ccagtaaaaa taaccagaaa atcatcagga aaaactgcct
    42901 tccaccttgt tccattcggc ttaaataaga ctcacgacct tctagaggtg gaagatgctt
    42961 cagaagcaat tcggctggtg attgatgaac gtcattccat tcgctgcagt acacttacag
    43021 caactattac gaataaaaag ggcaagcgaa ttaagcggac aggtatatac agtatcaagg
    43081 gtgttaacat aaaagaatac aacgtcaggt aatggcacag agcggtccgt cagttcaggt
    43141 ttggctctgt gccaaagatg ggtcaggtca cacctgatct aataaattta agccattaaa
    43201 tcagccttaa ccttcaccac caccttttta atctcacccg gctcgccaac aacgcatccc
    43261 ggcttatgcg gctctcctag cataaacacg gcgaacattc ccggtttcag gatgatggct
    43321 tgctcgtttt caatggtgct gcaaagctgg taatcatcct catggtggaa ctcttcacac
    43381 tgacgcgcag tgcctgccat gccaaacaga atccgttctt caccgtttaa taacagctgg
    43441 atatcaatgt attgctcgtg cagctccgct tttttctcaa cgggcgattg agtgttaaac
    43501 gtcatgacat tcataaaaat attgtcgccc tgtaattcgt aacgacccgg cgctttttct
    43561 tgcggtctgg cagctaatgc cagcgttaac gcgtcctgta acgcaggatg taacccagca
    43621 gacggtaatg accgtacttc acccatcatc ataatttttc tccctgggcc aacagcgctg
    43681 ccccaagtaa acctgcatca tggcggtagt gcgccgccag taaatcaaca tgaaataccg
    43741 ctggctcctg cgccagatac gtttccacca acgccagata cccttctgcc agaccaacgc
    43801 tgccaccgac caccacgcac tggcaatcag ttgtggcttt aatatcagcg atcagccttg
    43861 ccagcacatg tgcggagcgg tgaatcagtt gctgcgcctg ctcgtcgccc tgcccggcgc
    43921 gtgtgaaaat agttctcgca tccgcgccca tgagttcacc ttgcgctgcc gctgcaatac
    43981 cgcgaccaga agcaatcgct tccacgcaac ctgtgcgtcc acagccgcag actgggccgt
    44041 gtggatcggc aagcgtatgc ccgatatgcc ccgccagacc gccagggccg gtaagcagtt
    44101 taccgccgct cactacaccg ccgccgacgc cagtggaaac ggtgataaac accatctcgg
    44161 ttacatcgcc ttccagcgcc tgatactctg cccatgctgc ggcctgcgcg tcgttaatgg
    44221 caatggtcgg caaattggta agttgttcca gcgttttgac taacggaaag tgtagcaatc
    44281 caccgagatt atgcggattc agcgccagca aactgccgtc gcgaataatt ccggttgaag
    44341 cgatggcaac ccgctgcgca tgagcttgca acggagagac taatgcggat aaggcatcac
    44401 gcaaggcttc tggtgtctgg ctggctggcg taggaagttc acgacgatcg cggatctgcc
    44461 cgtcagcgcc aatcagcgcg gcggcaagtt tagtaccgcc gatatcaatc gccagtgtgg
    44521 tcatagcacc gcctttttca tcgctgtgtt gtaccactga caaatgtgtt caagacgcgt
    44581 aatggcagaa ccgaccgtca ccgcccacgc gccgtggcgc atcgcatctg ccgcctgagc
    44641 aggcgtgttg taacgccctt cggcaatcac ccgacatccg gcttcgctca acgttttcac
    44701 cagcgccaga tccggctctt ctggcgtttc aggcgtggta tagccagaaa gcgtagtgcc
    44761 aataatttcg gctcccagct tttggcatgc caggccgtct tccggcgttg agcagtcggt
    44821 catcgccagt aaaccgtgat gatgtatacg tgccagcagc gtttcaacag gcaccggacg
    44881 cgggcggtcg gtgccgtcaa tggcgataat gtccgcgccc gcctgcgcca gcgcatcgac
    44941 atcttcaata taggccgtga tgcgtaccgg agaatcctcc agatcgcgtt tcacaattcc
    45001 aataatcggc acgctcacca ccgcacgcgt ggcttgcaga tttgccacac cttcgatgcg
    45061 aatggcaacc gcgcccgcct gctctgccgc taatgccatg gctgcgacga tttcgggttt
    45121 atcgagcggg ctgtcaggaa tcggctggca ggagacaatc aggccaccgt tagcagcgat
    45181 tttttgatcc agttgtgcaa gtaacgacat acatcttccc ttagcgaaag gcccggtaca
    45241 tagaccgggc aacaggatta acttttggtt ttgactaaat cgtttttggc gctgccaaat
    45301 ggcacggcac cgctaaatgg tttgccgtcg atagcgtcat gagtacgcaa tgcttccggg
    45361 cgcagccaac gctgaacgcg ggaaggcata tccagaccaa tcagcaggat caccacaaat
    45421 gtcaggctga acgagagcga tgccagcgca gtacccagat ccagacgttg agcgatcaac
    45481 gcgccgatga ttggggccag tgcgccaccc aatgcgccaa cgttgtaggt aaagcccagg
    45541 cccgctgcac gctggtcggt atcgaaataa ccgccaatca gtttcggtaa gatcccggcg
    45601 atcccttgtc caagcatttg ctggaagaac agtaacagac cgagcaccca gacgtttgcg
    45661 ccgccaatcg caaataccgg aataatcagc agctgcgagg ccagcaggct acaaacgtac
    45721 gctttgcggg ttcccagcca gtcaccgagg aagcctccta cgcagcatcc cacggccgcg
    45781 ccaaagccac taaagaacag cacattggcg acagtatgcg ggttataagc cagatcggtt
    45841 ttcagatacg ttggcagcag cgcctgaatc ggccatgagt agaggaaagc aaacaacacg
    45901 accaccatca gcattacgcc cgttggccag cgtttgcctg tactctgcac cataaagctg
    45961 ataaagattg cggcgcataa cagcccaaga acagcgacga tcgcggcatt ttgcaggtta
    46021 ccggcgaagc agaaccacag cgcagtagcc gccgccagtg tcattacgat attggcaatg
    46081 cgatgttcac cacggtagag aatatccacc attgtgcgta ctggtgcttt acctgcgtgt
    46141 ttctctttcc agtcttccgc ttccgggatg tttttacgca gccagagagc aaagatgatt
    46201 ggcaaaatgc cgataaagaa cagcgcacgc caaccccaga ccggaaccac caggctatag
    46261 acctgagcgg caacgacggc ccccacagag aagcctgaaa tcaaaaaacc actggctttg
    46321 ttacgcagat gttttggcca gctttcaatg acataggtgg cgctggaacc gtattcaccc
    46381 gccatcccca tgccgatgac cagacgagcg ataaacatgg tgatgtagcc tggcgcaaag
    46441 ccgcaggcca gcgtcccggc cgagaagaga acgatgctgg tgaccattgc cagacgacgc
    46501 ccgtagcggt cacccatagc gccgagcatc aggccgccga accagcgaga gataaaggct
    46561 gcggagatca gacttgccgc ctgcaccgtc gtcagcccga attcaccctg tacttcggtg
    46621 agtaccaggg cgattaaaac gaaatcaaaa ccgtcaagca gatatcccaa ccaggcagcg
    46681 gaaaatgcgc gccattgtgc acggttgaga tggcgatacc acgggatatt ctgggttgta
    46741 gtactcattg tgagtctccc gcggtgggcg atgcccacac gctttggtat gaaaattgta
    46801 gggtacagat gcgtttattt cccctcaccc ggtaggggcg agcgagggga aaacaactca
    46861 cccgcgctct tgcatcaact gctgggccag cgccttcagt tctggcagat atttttcatc
    46921 taccggtcca aacggtttgc ggcacagcgg cacagaaacg acatccatat aatgcaggac
    46981 agttttcagg ccgcggaata cgccagtttt gatcagtaaa tcaatgactt tgttgcactc
    47041 agtttgcagt ttctgcgcgg tctggatatc gccttctttc agcgccttca cgatcccctg
    47101 atatcgccag cccatgatgt tgtaagtact gccgatacca ccatcagcgc ctgccagcaa
    47161 gccggaagcg aagatttcgt cgtaaccgtt atagagcacc agatcaggat gttcacgacg
    47221 gatctgttcc atctgataga gatcgccaga agtctgtttc agcgcaccga cgccaggcaa
    47281 cgtaaccagg gtgttgatct gatccagggt cagttttacc ccactcaggg ctggaatgtt
    47341 gtacacaacc atcggcaaac catccgctga atcaataatt gcccgatagt gatcgcagtg
    47401 ttcttcaaag ctgaaaggat agtagaacgg cgtgacggcg gagacggcat cgaagccata
    47461 acgtttagcc gatgccgcaa gttgttggct ttcggcggtg ctgacgcaac cgacgtgggc
    47521 gatgagttta atcttacctt tcgcctcttc ggcgacgatt tccagtacct gttcacgctc
    47581 ggaaaggctt tgtacaaagg cctcgccggt cgaaccaccc acgtataaac cgtcgatgcc
    47641 ctgctgaata ttgaactgaa ccaggcgacg cagactcgct ttatccagtg cttgttgttg
    47701 gtcaaaagga gtcaggagtg cagccattac gccacgtaaa ttcgttgcca taaatacctc
    47761 tgaagtgata cttgtctgat aaacgatata cctttatacc tgttatacca gatcaattaa
    47821 gcaacacccc atacagaaag cttataatgc gatctgcttc actaaagtgg cattatttct
    47881 ttttgttggt ggtctgaccg aaagcgtgcc aggtagcaga gacgctgttg agatgcgatt
    47941 gcaacgcacg atcggcttcg tcaggatcat gacggcggat cgcatcaacg atcgcaatat
    48001 gctgttgata gctaacgttg ttatgctcgt gcagagcctg atcagccacg gttggacgtg
    48061 cggcgatcag ccagtcgagc agggcaacgt ggatcgccat gaagattggg ttaccaggga
    48121 tctccgccag cacgcggtgg aaatcaacgt ctgaacgaat gaatgccgcg ttgttatcca
    48181 gcgactgact gttgatttcc agtgcttttg ccagcaaatc gatttgctca tcggtggcat
    48241 gttcagccgc atagcgcacc agactggatt caaagaacag acgtaattgt tcgaaatggg
    48301 caatcccacc gggatgagaa aggaaatctt tcgccatgcc ggaaaactca ccgatgatag
    48361 tgtccgcaga aggacgcgag acgcgagcgc gttcgccgtt gtttatttgc accagacctt
    48421 tgcgttttaa cgctgccagc gcttcgcgca cagaaggacg cccgacgtta aagaacgcca
    48481 tcagttcgcg ttcagacggt aattgttcac cttcgccaaa ttcacgacgg cggatcatct
    48541 gttccagctc ttcttccacc atttcggaga gttttttacg cgccagcggg cggctacgca
    48601 agttgcgacc aattgcaggt gaagaatctt cggtttgcga atcaaatgcg ttcataaggc
    48661 ccattctgta aggtcagtgt gattaacatc atcagtgaca tcctatcaca ggattgaaag
    48721 taggggaaaa tggcaggttt ttctctttgt gcctcatcat taccataatt aacggaataa
    48781 ttaactattg cgaaaaatta atgtaacgca gataaacaca tcccgattga attattcata
    48841 agactattca caagcattat gaatattatg aatgtgttct tacaaaataa tcataagcgc
    48901 atatttttta atgaaaaatc acatcaccta caattaaaaa cacgacatcc gcaccataaa
    48961 tagacttgca aaaaatataa catcattgtt ttcaatctgc cgtttatggg attgaccgtt
    49021 ttcttttgac acggagttca acaatgttcg gcataattat atctgtcatc gtattaatta
    49081 cgatgggcta tttgatcctg aaaaactaca aacctcaggt ggtgctggct gccgcaggtg
    49141 tcttcctgat gatgtgcgga gtctggttag ggttcggtgg tgtactcgat cccgccaaaa
    49201 gcagcggcta cttgatcgtc gacatttata atgaaatcct gcgcatgctg tccaaccgca
    49261 ttgccggatt ggggctgtcg attatggcgg tgggcggtta tgcccgctac atggagcgca
    49321 taggagccag tcgcgcgatg gtgagcttgc taagccgccc gttaaaactc attcgctcgc
    49381 cgtatattat tctgtcggca acttacgtca tcggccaaat catggcgcag tttattacca
    49441 gcgcctccgg tctgggtatg ttgctgatgg tcaccttatt tccgacgctg gtgagtctgg
    49501 gagtaagtcg cctctctgcg gtggcggtta tcgcaaccac gatgtccatt gagtggggga
    49561 ttctggaaac gaactccatt tttgcagccc aggtcgcggg aatgaaaatt gccacatact
    49621 tcttccacta ccagcttccg gtcgcctctt gcgtcattat ctcggtggcg atctcccact
    49681 ttttcgtgca acgcgctttt gacaaaaaag ataaaaatat caatcacgaa caggcagagc
    49741 aaaaaactct cgataatgtc ccgccgctct attacgccat tttacctgtg atgccgttaa
    49801 tcctgatgct cggctcgctg ttcctcgccc acgtcgggct gatgaagtca gaactgcatc
    49861 tggtggtggt gatgttacta agtttgacgg tgacgatgtt tgttgagttc ttccgcaagc
    49921 ataacttgcg cgaaacaatg gacgatgtgc aggcgttttt tgacggcatg ggtacgcagt
    49981 ttgccaacgt ggtaacgctg gtggtcgcgg gtgaaatatt tgcgaaaggc ttaacgacga
    50041 ttggcactgt tgatgcggtt atcaggggtg cggagcattc tggtctgggc ggtattggcg
    50101 tgatgattat tatggcgcta gtcattgcca tttgtgccat tgtgatgggc tctggcaatg
    50161 cgccgtttat gtcatttgcc agtcttattc cgaatatcgc agccggacta catgtaccag
    50221 cggttgtaat gattatgccg atgcattttg ccacgacgct agcgcgcgcg gtttcgccga
    50281 ttactgcggt ggtgaagcgg acagcgatcc ccatggcagt cggtttcgtg gtgaatatga
    50341 ttgccacaat cacgctattt tattaagtca ttaaaaagac aaaacaggcc gcctgggcct
    50401 gttttgtatt acttcacaac acgtaatgcc ggtcgaccac cgcgtggtgg ctgcggaggt
    50461 tcatcgtcag gatgagtgtc atcatcgtga tctggcttgt cgccatcaat aaccgacata
    50521 acggtttcgt tgtctgccga tgcctcttca tcattcatga tgctggtatc ttcatcgtag
    50581 gcagcttcag gctcaaacat cgtgcctgcg ccattttcac gggcgtagat agccagcacg
    50641 gcagccagcg gcacagaaac ctgacgcgga atgccaccaa agcgcgcgtt aaagcgcacc
    50701 tcatcattcg ccagttccag attgccgaca gcacgcggcg caatgttgag tacgatttgc
    50761 ccgtcacgcg catattccat aggaacctgc acgccaggga gcgtcacatc caccaccagg
    50821 tgcggcgtga gctggttatc cagcaaccac tcatagaatg cacgcagcag atagggacga
    50881 cgtggtgtta gctgtgacaa atccatacag attaactccg gcccagacgc atttcacgtt
    50941 ctgcttcagt taaagaagca aggaaagagt cacgctcaaa gacgcgggtc atatagcctt
    51001 tcagctcttt cgcacccggg ccgctgagct cgatacccag ttgcggcaga cgccacagca
    51061 gcggagcaag atagcaatcg accaggctga actcatcgct caggaagtac ggcttctgac
    51121 cgaagaccgg cgcaatcgcc agcagttctt cgcgcagttg cttacgtgcg gcatctgctt
    51181 cagaagctga accgttgatg atggtgttca tcagcgtgta ccagtctttt tcgatgcgat
    51241 gcatgtacag acggctttca ccgcgagcta ccgggtaaac aggcatcagt ggcggatgcg
    51301 ggaaacgctc atccagatat tccataatga tgcgagattc ccacagggtc agctcacgat
    51361 ccaccagggt cggaacgctc tgattcgggt tgaggtcaat cagatcctga ggcggattgt
    51421 ccttttccac gtgttcgatc tcgaaactta cacctttctc agccagcaca atgcggacct
    51481 gatggctata gatgtcagta ggaccggaaa acagcgtcat taccgaacgt ttgttggcag
    51541 cgacagccat gaaaacctcc aggtatagtc agaattttta ctgctaccag ccaccaggtg
    51601 gccagtcaga agttgtgtta cccaataagg aacgactctc tttgttcgaa aatcaaacaa
    51661 aaaatgagca atacccgaca tttgggcaga aaattggatg atagtttacc agattttgcg
    51721 accattgtgg tgagtggatg ccggaaatgg ggaaaaagag atgcgcttta gtctgaaata
    51781 gttgacttag acccttattg gcgatgtggt ttttgttttg cctgtctttc agacggcagc
    51841 aaaaagcaac ttcccatttt ttacgctgat tcagaattta gccataaaaa aacccgccga
    51901 agcgggtttt ttcgtaaatt gttttctgcc ggagcagaag ccaattaacg tttggagaac
    51961 tgcggacgac gacgtgcttt acgcagaccg actttcttac gttcaacctg acgagcgtca
    52021 cgagtaacga agccagcttt acgcagttca gaacgcaggg actcgtcgta ttccatcaga
    52081 gcgcgggtga taccgtgacg gatcgcacca gcctgaccag agataccacc acctttaacg
    52141 gtgatgtaca ggtccagttt ctcaaccatg tcgaccagtt ccagcggctg acgaactacc
    52201 atgcgggcag tttcacgacc gaagtactgt tccagagaac gttggttgat tacgatttta
    52261 ccgttgcccg gtttgatgaa aacgcgagct gcggaatttt tgcggcgacc agtgccgtag
    52321 tattgatttt cagccattgc ctataatccc gattagatgt caagaacttg cggttgctgt
    52381 gccgcgtggt tgtgctcgtt acccgcgtaa actttcagtt tacggaacat agcacgaccc
    52441 agcgggcctt ttggcaacat gcctttaacc gcgatttcaa tcacacgctc aggacggcga
    52501 gcaatcatct cttcaaagct cacttgtttg ataccaccga tgtggccggt gtggtgatag
    52561 tacactttgt cagtacgctt gttgccggtt acagcaactt tgtcagcgtt cagaacgatg
    52621 atgtaatcac cggtatctac gtgcggagtg tattccgctt tgtgcttacc gcgtaggcga
    52681 cgagccagtt cagtagccag acggcccaga gttttaccgg tcgcgtcaac aacataccag
    52741 tcgcgtttta cggtttctgg tttagctgta aaagttttca ttaaaagctt acccaataaa
    52801 tagttacacg ttggtgaaca cccaaacgtc ttcaattgtt gaggttcaca cgacaaagtc
    52861 cggcaaacct accccttcga atagcctatg ccagcacaca aaaagttttg ggaaaaaaac
    52921 tttcttgtaa cgtggggtcg caggattata gagaagtcgg ggtcaaagat cgaccccttt
    52981 ttgtgatttg tgacaggttt taacccgcca aatgctcgcg cttcagatac tcttcgcttt
    53041 gcatctcttg cagacgtgac aggcaacgct ggaactcaaa cttcagccga tcgccctgat
    53101 aaatttcata cagcggcact tctgcactca ccactaattt gacatggcgc tcgtaaaact
    53161 catccaccag cgcaataaag cgccgcgctt cgctctccat caaccgcgtc ataactggta
    53221 catcaaacaa catgaccgta tgaaagagac gtgagagcgc aatatagtca tgctgactgc
    53281 gggcgtcgac gcacagcgta gtaaaagaga ccgccagcgt ctggttctcg acgcccattg
    53341 tcgctaatgg ccgatggttg atttctaacg tcggtgaatt ttctcgtttc ccccccgcca
    53401 gcgccaacca tagtttatcc atttgcgccc gggtttcatc gtggagtggc gaaagccaca
    53461 gatgcgcctg agtgagtgta cgcagtcgat aatcaacacc agcgtccacg ttcattacat
    53521 cacaatgctg tttaatggca tcgattgcag gcagaaaacg cgcacgttgc aagccatttc
    53581 gataaagttc gtccggcgga atatttgacg tcgctaccag ggtaataccg cgggcgaaca
    53641 aggctttcat cagaccgcca agtagcatgg catcggtaat atcagaaaca aaaaattcgt
    53701 caaaacagag cacgtcagtt tcggctttaa agcgatcggc aataatttcc agcggatcgg
    53761 tctgcccctg taaggcagtt agctcttcat gcacccgcag cataaaacgg tgaaagtgca
    53821 ggcgctgttt ccgctctccc ggcaggcttt gatagaaaag gtccatcagc caggttttcc
    53881 cgcgtcctac accgccccac atatataagc cacgcactgg cgtatgcttt gtgtcttcgc
    53941 gtttacccca cagcttaccg acccgcgcca ttagcccact cgtcctgggg gctggtggcg
    54001 tgctattgat gagttcctga taaataattt ccaggcggct gacggcctct ttttgaacgt
    54061 cgtcgggttg atggctgcct tcattgagcg ccttcaggta ttgcgatgtt ggggtaacgc
    54121 tttgcatgat cttattgtta ttccttgaat aatcggtgcg ccgttgttca cggttgacga
    54181 aaaaaaggcc gttctacact acgcgatatg cagtcgggat tccacttctg tggaattaac
    54241 ggttatagtg gcataatcag ccgcaggcat ggagcctgaa gccaacaccc tacggaaaca
    54301 aaagacaacg ggagatgttc atgacctggg aatatgcgct aattgggtta gtcgtcggca
    54361 tcattattgg tgctgtggcc atgcgttttg gtaatcgtaa actacgccag caacaggcgt
    54421 tgcagtacga actggaaaag aataaagctg aactggacga gtatcgcgaa gagctggtta
    54481 gccactttgc ccgcagcgcg gaattactgg ataccatggc gcacgactat cgccagctgt
    54541 atcagcacat ggcgaaaagt tccagcagcc tgctgccgga actgtctgct gaagcaaacc
    54601 cgttccgtaa tcgtctggca gagtctgaag ccagcaacga tcaggcaccg gtgcagatgc
    54661 ctcgcgacta ttctgaaggc gcatccggcc tgctgcgtac tggcgcgaag cgcgactaat
    54721 ttatttttcg ggcgcagcca ttgcgccctc ctcttctctc cctccccgac tatcatttaa
    54781 tctggtgtct cattgttagc cgtctgaaaa ttcaataaca tcaaactgtt ttgaatctct
    54841 tttcttatca ttcaggtacg agagcaggaa taatgaaaaa acaaccccag ctgttgagtg
    54901 cattagcgtt aagtgtcggg ttaactctct cggcgtcatt tcaggccgtc gcgtcgattc
    54961 caggccaggt tgccgatcag gcccctctcc ccagtctggc tccaatgctg gaaaaagtgc
    55021 ttccggcagt ggtgagcgta cgggtggaag gaacggccag tcagggacag aaaatcccgg
    55081 aagaattcaa aaagtttttt ggtgatgatt taccggagca acctgcacaa cccttcgaag
    55141 gtttaggctc cggtgtcatc atcaacgcca gtaaaggcta tgtgctgacc aacaaccatg
    55201 tgattaatca ggcacagaaa atcagtattc agctcaatga tgggcgcgag tttgatgcaa
    55261 aactgattgg tagcgatgac cagagcgata tcgccctgtt acaaattcaa aacccgagca
    55321 aattaacgca aatcgctatt gccgactccg ataaattgcg cgtcggtgat tttgccgtag
    55381 cggtcggtaa cccatttggc cttgggcaaa ccgccacctc tggcattgtt tccgcattag
    55441 gacgcagcgg gttgaatctt gaaggtctgg aaaactttat ccagacagat gcttccatta
    55501 accgcggtaa ctccggcggt gcactgttaa accttaacgg tgagttaatt ggcatcaaca
    55561 ctgcaatcct tgcgcctggc ggcgggagcg tcgggattgg atttgccatc cccagtaata
    55621 tggcgcgaac actggcgcag cagcttatcg actttggtga aatcaaacgc ggtttgttag
    55681 gcatcaaagg caccgagatg agtgccgata tcgccaaagc cttcaacctt gacgtgcagc
    55741 gtggcgcgtt tgtcagcgaa gtgttgccag gttctggctc ggcgaaagcg ggcgtcaaag
    55801 cgggcgatat tattaccagc ctcaacggca aaccgctgaa tagctttgct gagttgcgct
    55861 ctcgtatcgc gaccaccgag ccgggcacaa aagtgaaact tggcctgctg cgtaacggca
    55921 aaccactgga agtagaagtg acgctcgata ccagcacctc ttcgtcggcc agcgctgaaa
    55981 tgatcgcgcc agcgctggaa ggtgcaacgt tgagcgatgg tcagctaaaa gatggcggca
    56041 aaggtattaa gatcgatgaa gttgtcaaag gaagcccagc tgctcaggct ggcttgcaaa
    56101 aagacgatgt gatcattggc gtcaaccgcg atcgggtgaa ctcgattgct gaaatgcgta
    56161 aagtgctggc ggcaaaatcg gccatcatcg ccctgcaaat tgtacgcggc aatgaaagca
    56221 tctatctgct gatgcgttaa tgtcgtaaac cgggcatcag gcttacgtgt gatgtccggt
    56281 taactcgtgg tatgctgctg ccgttccctt ttttaatgac gcctccatca tgtttgtgaa
    56341 gctcttacgt tccgttgcga ttggattaat tgtcggcgct attctgctgg ttgccatgcc
    56401 ttcgctgcgc agccttaacc cgctttccac tccgcaattt gacagtaccg atgagacgcc
    56461 tgccagctat aatctggcgg ttcgccgcgc cgcgccagcg gtggttaacg tttacaaccg
    56521 tggtttgaac accaactctc acaaccagct tgagatccgc accctgggat ccggtgtaat
    56581 catggatcaa cgcggttata tcatcaccaa taaacacgtc atcaacgacg ccgatcagat
    56641 catcgtcgcc ttacaggatg gtcgtgtatt tgaagcattg ctggtgggat ctgactctct
    56701 aaccgatctg gcggtactta aaattaatgc cactggcggt ttacctacca ttccaatcaa
    56761 tgcacgtcgc gtaccgcata ttggcgacgt ggtactggcg atcggtaacc cgtacaacct
    56821 cgggcagacc attacccagg ggattattag tgccacgggt cgaattggtc tgaacccgac
    56881 cgggcggcaa aacttcctgc aaaccgatgc ttccattaac cacggtaact ctggcggcgc
    56941 gctggtgaac tcgctgggcg aactgatggg cattaacacg ctgtcgtttg ataagagtaa
    57001 cgatggcgaa acgccggaag gtatcggctt tgcgattcct ttccagttag caaccaaaat
    57061 tatggataag ctgatccgcg atggtcgcgt gatccgcggc tacattggca ttggcggacg
    57121 tgagatcgca ccgctgcacg cacagggcgg tggtatagat caactgcaag ggatcgtggt
    57181 taatgaagtg tcacctgacg gcccggcggc gaatgcgggt attcaggtca acgatctgat
    57241 tatttcggtg gataacaaac cggccatctc tgctctggag acgatggatc aggtggcgga
    57301 aattcgccct ggttcggtga tcccggttgt agtgatgcgt gatgataagc agttaacgct
    57361 gcaggtcacc attcaggaat atccggcaac caattaagtc gagtgctcaa aacaaaaaac
    57421 cggagtctgt g
//
LOCUS       DADMKF010000008        49564 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.008, whole genome shotgun sequence.
ACCESSION   DADMKF010000008 DADMKF010000000
VERSION     DADMKF010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 49564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49564
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.008"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            114..746
                     /gene="comR"
                     /locus_tag="JNC24_000451"
     CDS             114..746
                     /gene="comR"
                     /locus_tag="JNC24_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HAY9157296.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(808..1347)
                     /locus_tag="JNC24_000452"
     CDS             complement(808..1347)
                     /locus_tag="JNC24_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HAY9157297.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(1574..2878)
                     /locus_tag="JNC24_000453"
     CDS             complement(1574..2878)
                     /locus_tag="JNC24_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAY9157298.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELYNAVKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMKNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(3286..3828)
                     /locus_tag="JNC24_000454"
     CDS             complement(3286..3828)
                     /locus_tag="JNC24_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAY9157299.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(3851..4876)
                     /gene="nagZ"
                     /locus_tag="JNC24_000455"
     CDS             complement(3851..4876)
                     /gene="nagZ"
                     /locus_tag="JNC24_000455"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HAY9157300.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSSYWLKTVLRQELCFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKAGH"
     gene            complement(4887..5711)
                     /gene="thiK"
                     /locus_tag="JNC24_000456"
     CDS             complement(4887..5711)
                     /gene="thiK"
                     /locus_tag="JNC24_000456"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HAY9157301.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPASIAPKPHLYLRDWMVVDYLPGAVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKAGWFEYRWRQIGDQQFIRLADDTWRQLLIKQ"
     gene            complement(5692..6333)
                     /gene="lpoB"
                     /locus_tag="JNC24_000457"
     CDS             complement(5692..6333)
                     /gene="lpoB"
                     /locus_tag="JNC24_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HAY9157302.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDQTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(6347..6724)
                     /locus_tag="JNC24_000458"
     CDS             complement(6347..6724)
                     /locus_tag="JNC24_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HAY9157303.1"
                     /translation="MRKGCFGLVSLALLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVSIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(6727..7086)
                     /gene="hinT"
                     /locus_tag="JNC24_000459"
     CDS             complement(6727..7086)
                     /gene="hinT"
                     /locus_tag="JNC24_000459"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HAY9157304.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            7420..9609
                     /gene="fhuE"
                     /locus_tag="JNC24_000460"
     CDS             7420..9609
                     /gene="fhuE"
                     /locus_tag="JNC24_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
                     /protein_id="HAY9157305.1"
                     /translation="MLSTQFNRDNQYQAITKPSLLAGCIALALLPSAAFAAPATEETV
                     IVEGSATAPDDGENDYSVTSTSAGTKMQMTQLDIPQSVTIVSQQRMEDQQLQTLGEVM
                     ENTLGISKSQADSDRALYYSRGFQIDNYMVDGIPTYFESRWNLGDALSDMALFERVEV
                     VRGATGLMTGTGNPSAAINMVRKHATSREFKGDVSAEYGSWNKERYVADLQSPLTEDG
                     KIRARIVGGYQNNDSWLDRYNSEKTFFSGIVDADLGDLTTLSAGYEYQRIDVNSPTWG
                     GLPRWNTDGSSNSYDRARSTAPDWAYNDKEINKVFMTLKQRFADTWQATLNATHSEVE
                     FDSKMMYVDAYVNKADGMLVGPYSNYGPGFDYVGGTGWNSGKRKVDALDLFADGSYEL
                     FGRQHNLMFGGSYSKQNNRYFSSWANIFPDEIGSFYNFNGNFPQTDWSPQSLAQDDTT
                     HMKSLYAATRVTLADPLHLILGARYTNWRVDTLTYSMEKNHTTPYAGLVFDINDNWST
                     YASYTSIFQPQNDRDSSGKYLAPITGNNYELGLKSDWMNSRLTTTLAIFRIEQDNVAQ
                     STGTPIPGSNGETAYKAVDGTVSKGVEFELNGAITDNWQLTFGATRYIAEDNEGNAVN
                     PNLPRTTVKMFTSYRLPVMPELTVGGGVNWQNRVYTDTVTPYGTFRAEQGSYALVDLF
                     TRYQVTKNFSLQGNVNNLFDKTYDTNMEGSIVYGAPRNFSITGTYQF"
     gene            complement(9669..11102)
                     /gene="ptsG"
                     /locus_tag="JNC24_000461"
     CDS             complement(9669..11102)
                     /gene="ptsG"
                     /locus_tag="JNC24_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HAY9157306.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFSFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(11397..12194)
                     /locus_tag="JNC24_000462"
     CDS             complement(11397..12194)
                     /locus_tag="JNC24_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAY9157307.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(12205..13209)
                     /gene="holB"
                     /locus_tag="JNC24_000463"
     CDS             complement(12205..13209)
                     /gene="holB"
                     /locus_tag="JNC24_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HAY9157308.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVVE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(13206..13847)
                     /locus_tag="JNC24_000464"
     CDS             complement(13206..13847)
                     /locus_tag="JNC24_000464"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HAY9157309.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(13837..14859)
                     /gene="yceG"
                     /locus_tag="JNC24_000465"
     CDS             complement(13837..14859)
                     /gene="yceG"
                     /locus_tag="JNC24_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HAY9157310.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTWMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(14862..15671)
                     /gene="pabC"
                     /locus_tag="JNC24_000466"
     CDS             complement(14862..15671)
                     /gene="pabC"
                     /locus_tag="JNC24_000466"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HAY9157311.1"
                     /translation="MFLINGHKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMIFCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTVYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVSFSSATLYEYLAPLCERPN"
     gene            complement(15791..17032)
                     /gene="fabF"
                     /locus_tag="JNC24_000467"
     CDS             complement(15791..17032)
                     /gene="fabF"
                     /locus_tag="JNC24_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HAY9157312.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLDITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(17120..17356)
                     /gene="acpP"
                     /locus_tag="JNC24_000468"
     CDS             complement(17120..17356)
                     /gene="acpP"
                     /locus_tag="JNC24_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAY9157313.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(17567..18301)
                     /gene="fabG"
                     /locus_tag="JNC24_000469"
     CDS             complement(17567..18301)
                     /gene="fabG"
                     /locus_tag="JNC24_000469"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HAY9157314.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(18314..19243)
                     /gene="fabD"
                     /locus_tag="JNC24_000470"
     CDS             complement(18314..19243)
                     /gene="fabD"
                     /locus_tag="JNC24_000470"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAY9157315.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKLMQEAVPEGTGAMAAIIGLDDASIAKACEEAAAGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVSVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(19259..20212)
                     /locus_tag="JNC24_000471"
     CDS             complement(19259..20212)
                     /locus_tag="JNC24_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HAY9157316.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(20280..21350)
                     /gene="plsX"
                     /locus_tag="JNC24_000472"
     CDS             complement(20280..21350)
                     /gene="plsX"
                     /locus_tag="JNC24_000472"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HAY9157317.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRVALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(21431..21604)
                     /gene="rpmF"
                     /locus_tag="JNC24_000473"
     CDS             complement(21431..21604)
                     /gene="rpmF"
                     /locus_tag="JNC24_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAY9157318.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(21656..22177)
                     /gene="yceD"
                     /locus_tag="JNC24_000474"
     CDS             complement(21656..22177)
                     /gene="yceD"
                     /locus_tag="JNC24_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HAY9157319.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEVQK
                     PNPFAVLASLKRK"
     gene            22376..22960
                     /gene="yceF"
                     /locus_tag="JNC24_000475"
     CDS             22376..22960
                     /gene="yceF"
                     /locus_tag="JNC24_000475"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HAY9157320.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(23072..24031)
                     /gene="rluC"
                     /locus_tag="JNC24_000476"
     CDS             complement(23072..24031)
                     /gene="rluC"
                     /locus_tag="JNC24_000476"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HAY9157321.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLIAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            24604..27789
                     /gene="rne"
                     /locus_tag="JNC24_000477"
     CDS             24604..27789
                     /gene="rne"
                     /locus_tag="JNC24_000477"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HAY9157322.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAATVVAPAPKAATATPAAPA
                     QPGLLSRFFGALKALFSGGEEAKPTEQPTPKAEAKPERQQDRRKPRQSNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTGVPVAAAVEPVVSAPVVEEVAEVVEAPVQIAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVAAVEPEVTVEHNHATVPMTRAPAPEYVPDAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(27984..28937)
                     /gene="flgL"
                     /locus_tag="JNC24_000478"
     CDS             complement(27984..28937)
                     /gene="flgL"
                     /locus_tag="JNC24_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-filament junction protein FlgL"
                     /protein_id="HAY9157323.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTETAPFSEANGDYVGGTESI
                     KQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKETAAAALDKTNRGLKNSLNNVLTVRAELGTQLNELESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(28949..30592)
                     /gene="flgK"
                     /locus_tag="JNC24_000479"
     CDS             complement(28949..30592)
                     /gene="flgK"
                     /locus_tag="JNC24_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HAY9157324.1"
                     /translation="MSSLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQA
                     NSTLGAGGWVGNGVYVSGVQREYDAFITNQLRAAQTQSSGLTARYEQMSKIDNMLSTS
                     TSSLATQMQDFFTSLQTLVSNAEDPAARQALIGKSEGLVNQFKTTDQYLRDQDKQVNI
                     AIGASVDQINNYAKQIASLNDQISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVS
                     VQDGGTYNITVANGYSLVQGSTARQLAAVPSSADPSRTTVAYVDGTAGNIEIPEKLLN
                     TGSLGGILTFRSQELDQTRNTLGQLALAFAEAFNTQHKAGFDANGDAGEDFFTIGKPA
                     VLQNTKNKGDVAIGATVTDASVVLATDYKISFDNNQWQVTRLASNTTFTVTPDANGKV
                     AFDGLELTFTGTPAVNDSFTLKPVSDAIVNMDVLITDEAKIAMASEKDAGDSDNRNGQ
                     ALLDLQSNSKTVGGAKSFNDAYASLVSDIGNKTATLKTSSTTQGNVVTQLSNQQQSIS
                     GVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR"
     gene            complement(30658..31599)
                     /gene="flgJ"
                     /locus_tag="JNC24_000480"
     CDS             complement(30658..31599)
                     /gene="flgJ"
                     /locus_tag="JNC24_000480"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HAY9157325.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKAGEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQALSQLVQKAVPRNYDDSLPGDSKAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGPVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQALQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(31599..32696)
                     /locus_tag="JNC24_000481"
     CDS             complement(31599..32696)
                     /locus_tag="JNC24_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HAY9157326.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTESLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(32708..33406)
                     /gene="flgH"
                     /locus_tag="JNC24_000482"
     CDS             complement(32708..33406)
                     /gene="flgH"
                     /locus_tag="JNC24_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HAY9157327.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(33459..34231)
                     /gene="flgG"
                     /locus_tag="JNC24_000483"
                     /pseudo
     CDS             complement(33459..34231)
                     /gene="flgG"
                     /locus_tag="JNC24_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
     gene            complement(34403..35158)
                     /locus_tag="JNC24_000484"
     CDS             complement(34403..35158)
                     /locus_tag="JNC24_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgF"
                     /protein_id="HAY9157328.1"
                     /translation="MDHAIYTAMGAASQTLNQQAVTASNLANASTPGFRAQLNALRAV
                     PVEGLSLPTRTLVTASTPGADMTPGKMDYTSRPLDVALQQDGWLAVQTADGSEGYTRN
                     GSIQVDPTGQLTIQGHQVIGEAGPIAVPEGAEITIAADGTISALNPGDPANTVAPVGR
                     LKLVKATGSEVQRGDDGIFRLSAETQATRGPVLQADPTLRVMSGVLEGSNVNAVAAMS
                     DMIASARRFEMQMKVISSVDDNAGRANQLLSMS"
     gene            complement(35178..37103)
                     /gene="flgD"
                     /locus_tag="JNC24_000485"
                     /pseudo
     CDS             complement(35178..37103)
                     /gene="flgD"
                     /locus_tag="JNC24_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706995.3"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
     gene            complement(37115..37519)
                     /gene="flgC"
                     /locus_tag="JNC24_000486"
     CDS             complement(37115..37519)
                     /gene="flgC"
                     /locus_tag="JNC24_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HAY9157329.1"
                     /translation="MALLNIFDIAGSALTAQSQRLNVAASNLANADSVTGPDGQPYRA
                     KQVVFQVNAAPGAATGGVKVADVIESQAPDKLVYEPGNPLADAKGYVKMPNVDVVGEM
                     VNTMSASRSYQANVEVLNTVKSMMLKTLTLGQ"
     gene            complement(37523..37939)
                     /gene="flgB"
                     /locus_tag="JNC24_000487"
     CDS             complement(37523..37939)
                     /gene="flgB"
                     /locus_tag="JNC24_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HAY9157330.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            38094..38753
                     /gene="flgA"
                     /locus_tag="JNC24_000488"
     CDS             38094..38753
                     /gene="flgA"
                     /locus_tag="JNC24_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation protein
                     FlgA"
                     /protein_id="HAY9157331.1"
                     /translation="MLAIKRSVAIISILFTPLSAASNLTSQLQTFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRQDTLPPRTVLDINQLVDAVSLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVTQNARVRMVSGLVVSGVVDADGNILIN
                     Q"
     gene            38829..39122
                     /gene="flgM"
                     /locus_tag="JNC24_000489"
     CDS             38829..39122
                     /gene="flgM"
                     /locus_tag="JNC24_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-28 factor FlgM"
                     /protein_id="HAY9157332.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDASVTNTRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALLNEAQQDLQSN"
     gene            39127..39543
                     /gene="flgN"
                     /locus_tag="JNC24_000490"
     CDS             39127..39543
                     /gene="flgN"
                     /locus_tag="JNC24_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HAY9157333.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRRKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTTHRGGKKISI"
     gene            complement(39583..41118)
                     /gene="murJ"
                     /locus_tag="JNC24_000491"
     CDS             complement(39583..41118)
                     /gene="murJ"
                     /locus_tag="JNC24_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HAY9157334.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(41228..42151)
                     /locus_tag="JNC24_000492"
     CDS             complement(41228..42151)
                     /locus_tag="JNC24_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAY9157335.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWMLQGAWSPTRAKALP
                     ICESWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSADPLQITTCMHRRAG
                     SQRETVQAVTDDALIDITDMREWREERGQGVVHKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVLQTAGEQAVLAQRIVDKIWRDAMSE"
     gene            complement(42153..42800)
                     /locus_tag="JNC24_000493"
     CDS             complement(42153..42800)
                     /locus_tag="JNC24_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF480 domain-containing protein"
                     /protein_id="HAY9157336.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTDMSNAVDGDLQARVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(42811..43395)
                     /gene="rimJ"
                     /locus_tag="JNC24_000494"
     CDS             complement(42811..43395)
                     /gene="rimJ"
                     /locus_tag="JNC24_000494"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HAY9157337.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            43631..44839
                     /gene="mdtH"
                     /locus_tag="JNC24_000495"
     CDS             43631..44839
                     /gene="mdtH"
                     /locus_tag="JNC24_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HAY9157338.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRCRFFSLLMMQDSAGAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSALLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     ATRRLLERDA"
     gene            44903..45550
                     /gene="grxB"
                     /locus_tag="JNC24_000496"
     CDS             44903..45550
                     /gene="grxB"
                     /locus_tag="JNC24_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HAY9157339.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYRDNMAKQTQINLLSSMAI"
     gene            45684..46244
                     /locus_tag="JNC24_000497"
     CDS             45684..46244
                     /locus_tag="JNC24_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAY9157340.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLISQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            46350..47396
                     /gene="pyrC"
                     /locus_tag="JNC24_000498"
     CDS             46350..47396
                     /gene="pyrC"
                     /locus_tag="JNC24_000498"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HAY9157341.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHDFTPLMTCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            47470..47715
                     /gene="dinI"
                     /locus_tag="JNC24_000499"
     CDS             47470..47715
                     /gene="dinI"
                     /locus_tag="JNC24_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HAY9157342.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
     gene            48005..48259
                     /gene="bssS"
                     /locus_tag="JNC24_000500"
     CDS             48005..48259
                     /gene="bssS"
                     /locus_tag="JNC24_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415578.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HAY9157343.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFQVNEYRRH"
     gene            48374..49492
                     /gene="solA"
                     /locus_tag="JNC24_000501"
     CDS             48374..49492
                     /gene="solA"
                     /locus_tag="JNC24_000501"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HAY9157344.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETVDGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVVSDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDKKSDFDLTPFRLSRFQ"
BASE COUNT        12044 a        13341 c        12296 g        11883 t
ORIGIN      
        1 tttaaatttc agttaaatag aaagcaatta tttttatggg atctggactg gtgaacggag
       61 gtgattattt ttataataac ctttcattca cagtcaccag gttcaacggc atcatggcaa
      121 ctgactcaac acaatgtgta aaaaaaagcc gtggccgccc aaaagtgttc gacagggatg
      181 ccgcgcttga taaggccatg aaattgttct ggcaacacgg ttatgaagcg acttctcttg
      241 cggacctcgt cgaagcgacc ggagccaaag cgcccacgct atacgcggaa tttaccaaca
      301 aagaggggtt atttcgtgcc gttctcgacc gctatatcga tcgttttgcc gctaagcatg
      361 aagcacagct gttttgtgaa gagaaaagcg tggagtctgc gctggctgac tattttgctg
      421 ccatcgccaa ctgctttacc agcaaagaca ccccggcagg ctgcttcatg atcaacaact
      481 gcaccaccct ctccccagat tcaggagata tcgccaatac gttgaaatca cgccatgcga
      541 tgcaagagcg cactttgcag cagtttttat gtcaacgaca agcgcgcggg gaaatcccga
      601 cccactgtga cgtgacacat ctggcagaat tccttaattg tattattcag gggatgtcga
      661 tcagcgcacg cgaaggtgca tcgctggaaa aactgatgca gattgccgga acgactttgc
      721 gtttatggcc cgaactggtg aaataagtcg caaaaaaagc ccctcagcct gagcgaatga
      781 ggggccaaat tgcagagtac agccttgtta tactttgtta ttcaaaatca gttgcccgtt
      841 actatctagc gggatctgcg tacccggatc gcggtccatg cggattttgc cctgctgatc
      901 gccaatctta taggtcacat cataaccgag cattttttct gacttgtcat acaccgtttt
      961 acaacgctgt tgcgtagtcg tgtaagtatc gctttcctgg agagagccct ggatctggtt
     1021 accggcatat ccaccaccca gcgcccccac aacagtggcg acatctttac cacgaccacc
     1081 accaaactga tgccctatca cgccgccagc aacagcgccg agcaccgacc cggtaatgcg
     1141 attttcatcc tgcaccggtc gacgatgggt cactgtgacg ttgcgacact cctgacgcgg
     1201 tgttttaacc gtttccttga ttggggttgc agaaacaacc tgagcgtatt gcgggccccg
     1261 ttcaaacacg ttcagactgg ccactgccgc tacgcccagc gcagctgcga caccaatccc
     1321 gatacccgcc aacattgatt tattcacggg aacacctcct tctttgcagt tatgccaatc
     1381 atgcaagcga aggagaaaag cagctatcag acagaagatg aaaagcgcgg gaatggctgg
     1441 aaatacttaa ggattcggag aagtctgaag gaatgcctga tgcgacgctt aacgcgtctt
     1501 atcaggccta ccgctcgtac catttgtcca gggcggatga ggcgtttatg ccacatccgc
     1561 cagtgtacgt cgattaatgc aacttcaaac gcggacggat aacgcggtta atactcccca
     1621 ccagcatcat taatccggtt ttaaagtaac catgcagcgc aatctgatgc attcggtata
     1681 gcgagatata tacaaagcgc gcaattcgtc cttcaatcat cattgagccg cgcgtcaggt
     1741 tacccatcag gctaccgaca gtggagaagt tcgacagcga taccagcgaa ccatgatctt
     1801 tatactgata atttttcagc ggtttaccgt tcatctgcgc cagaatattt ttcattgcgc
     1861 aggtcgccat ctggtgtgca gcctgagcac gcggcggaac aaagccccct tccggacgcg
     1921 ggcatgacgc gcagtcgcca atagcgtaaa tgtctggatc gcgggtggtt tgcagcgtcg
     1981 gttccaccac cagctggttg atacggttgg tttcaagacc accgatatct ttcaggaagt
     2041 ctggcgcttt gatcccggct gcccacacca tcagatcagc ctcaatatat tcaccatctt
     2101 tagtgtgcag gccgccttca tcagcactgg tgaccatggt ttgcgtcagc acgcgaacgc
     2161 caagtttcgt tagctcgttg tgggccgcag cagagatacg tggcggtaat gcaggcaaaa
     2221 tacgttctcc cgcttctacc agcgttacgt tcagggcttc gttggtcagg cctttgtaac
     2281 cgtagctgtg cagttgcttg accgcattgt acaattcagc ggagagttct acacccgtcg
     2341 cgccgccgcc gacaatcgca atgttcactt taccattcgc gcccaggttg gcggagtatt
     2401 tcaggaacag attcagcatc tcctggtgga agcgacgcgc ctggtgcggg ttatcgagga
     2461 aaatgcagtt ctctttgaca cctggcgtat tgaaatcgtt agaggtgcta cccagcgcca
     2521 ttaccagggt gtcataggcg attttacgtt ccggaaccag cagttcacct ttctcatcgc
     2581 gcagttctgc aatagtgatt gttttcgctt cacgatcaat atcaatgacg gaacccagct
     2641 ggaactggaa accatgattg cgcgcatggg ccagatagct caacgcatcg acgccttcat
     2701 caagcgagcc agtcgccact tcgtgcagca gcggtttcca cagatggctg tggttacgat
     2761 cgaccagcgt aattttggct tttttcttgc gtcccagctt atgccccagc tgtgttgcca
     2821 tttccagccc accagcaccg ccgccgacaa tcacaatttt tttcaatggc gtagtcaacg
     2881 tgaccccctt aaatttatta accaattgtt aattaatggt tataaacata gctcttaatt
     2941 aacaacaggt tacagcaatg aaaatgttct tcgctattga gaataacatg aatggtgcat
     3001 tggtcatacc aaaattgatg ttaatcaagt tttgttgctg aaaagatagg cgactttacc
     3061 gggtgagatc aaaaaacaga gtttattgtt tattatttga gcaagtccgg aagggtgata
     3121 tagggtgacg cgtatatcga gggcgtttgg tcgtcctgat aagatgcgca gcatcgcatc
     3181 aggcattgat gctcgactgt cggatgcggc gtaaacgcct tatccgactt acagaattcg
     3241 gagcgcatca ggtaccggca aggttacctg atgcgacgag ggcatttacc cgagggtttt
     3301 gaacgcctta atgcgctgca aatgcgggga gatattcttg aatttgtgcg tctgctcttc
     3361 gtcccagaca atctcgtaat aatgatgcaa ctcttcagat gtccgctggc tgttaagcgc
     3421 ttcatcatta cgcgacaaaa tcaccaggca acgatcgcga ttcttctcgc ggaagttggt
     3481 cacacactta gtggcaatat cggcatactc ttccgggcga tcaatcttgc cttccatgtt
     3541 ctcataaggg aacaaattag ggttgaagat cacctggcgg atatcgcaaa gaaaaccaat
     3601 ccgttccgcc cagtatccgc ccagaccaac gccgcaaatc agcggacgct cgtcgacgtt
     3661 cagttgcaac attttgtcca cttctttgag cagatgctgc atatcatgtt tcggatgccg
     3721 cgtactgtag cttatcaagc gtacatccgg gtcaataaac tgcaattgta agactttctc
     3781 gtggttaccc ggactgttag agtcaaaacc gtgtaaatag ataatcatcg cttcctcaca
     3841 taagccaggg ttagtgacct gctttctctt cctgccagcg ttcatgtaac tgattcagac
     3901 gggtgctgat cgccttccag cgagccgagt ccatcagttc ctgtcgcgaa aatgaacctt
     3961 tatgatacaa acgtgtaaca cgttctgcct tgatcggtga cagattatct aacacgctga
     4021 cggccccttt acgattattg cagaccagga tcatatcgca acctgcgtcc agtgatgcct
     4081 gcccgcgttc ggcataactg cccataatcg cggcaccttc catcgataaa tcgtcagaga
     4141 aaattacacc gtcaaaacac agttcctgac gcaaaacggt tttcagccag taggaagaac
     4201 cgctcgccgg acgcggatca acatcactgt agatcacatg cgcaggcata atggcgtcga
     4261 gtttattttc gcggattaag gaactgaaga ccgacatatc tttagcgcga atctccgctt
     4321 gtggacgtgg atcgcacggt gtttctttgt gtgagtctgc cgttactgca ccgtgtcctg
     4381 ggaagtgttt cccggtcgtt ttcattccgg cttcatgcat accatcaata aaccggctgg
     4441 caattgccag ggctttttgt ggatcggcat gataagaacg ctcgccaatc gccgcgctga
     4501 tatgcccgac atccagcact ggcgcaaagc tgatatcaat atccatagcg atcatttcgc
     4561 tggccatcag ccaacccgcc tcttgcgcca gtttgccgcc ctcttccatt cccaacagcg
     4621 cagcaaagga ttgtgctgcc ggtaagcggg taaaaccttc gcgaaaacgc tgcacgcgtc
     4681 caccttcctg atctaccgcc accaccagat gattgcgcga tgctgcgcgg atctggcgca
     4741 ccagttcacg taactgggca ggatcatgat agttacgcgt aaagagaatc agccctccca
     4801 ccagcggatg cgccagtatt tcacgctctt ccgcgtccag ttcgtaacct tcgacatcca
     4861 acattactgg acccacactg ctctccttat tgttttatta atagctgccg ccaggtgtca
     4921 tcggccagcc tgataaattg ttgatcgccg atttgtcgcc agcggtactc aaaccaccct
     4981 gctttgagca tcagcagcca gggaaaccat cgcctgacct gacgccataa ttgcgccgga
     5041 taaatcttcg cgcgagtggc atagtcattg accaattgcc ggtgctgttc agtattttcc
     5101 acccacaccg ccgccagttc cagcgcgata tcaccatctc cggcatactc ccagtcgatg
     5161 agttttaacc ctgacgcgct atgcactaaa tttccggcgt ggacatccat atgcaatgga
     5221 cttaagcgta aaggccgtgg ttcccgcgct ttgcgcagac gttttaacat tcgcagccaa
     5281 cccacagtcc gccgcgccgg atcgctttgc tgccagtaca gttccagtaa gggcaacagc
     5341 gttattcgcc agccaaaacg tggttgttga tgaagataat acagcaagcc tgccagttcg
     5401 ttggtatccg gcaaatacgt ttttaccgcg ccgggcagat agtcgactac catccagtca
     5461 cggagatata aatgcggctt cggtgcaatg cttgcgggta gttgtgataa agcccgatac
     5521 tggcgcaaga acgcggactg cggcgcatca ggatcgtgcg gctgacgcac aacaaaacgc
     5581 tggccctgat gttcaatgag aaaactaccg ccactaagcc cactattaaa cgtcgtgacg
     5641 ggatgaaact gcgggaaaaa gcgcgacagc aattcgtcgc gcgtgatggg attattgctg
     5701 cgaaacggca cctttacctg accagataat ttcgcccgtc tgcaccagca tcagctgcat
     5761 ttgtagggtc ggagcgttaa cgttgccaga ggcgctggag tacagcacgt aatgagcgcc
     5821 gacattgcgg gcaatgccta tggctttact acgggtacct aaactgtcct gcggcgacaa
     5881 acctaactgt tgcttagcca tcgacagctg ctgggcggaa accagggtaa atttcccgtt
     5941 attagccagc gcatttcgca gcgtttcggt cgcttctgcg gcattcagcg aaccgttagt
     6001 acggttgtta acgctatcaa ccagcaggac gctacccgca gtcaccccgt cagccccaag
     6061 catcttactg accatcggct gcattgcgcc attccagtca taatggcgaa tatgcggcgc
     6121 aggcggtgcc gtttgatctt cgtgctcaat tgggcctggc tgctgcggga tcgtcggcac
     6181 cgagggcact gtggggacag gctgttgtgg ctcggctggt tgttccggcg ctggtttcac
     6241 ttcttctacc ggtgcaggtt cacgttgccc cacacacccg gcgagaaaca tcgccagcgc
     6301 ggtaatcaag gcgtagcgac tcatttttgt catcaagatt caccccttac aaatatagat
     6361 aaagtctgac tttgtgcgcc cccagaaaat tggcgctgcc gtacagcgtt accgccgaat
     6421 gtgcgggaat gctaacgctg cgtggccttt ccagaggatg catctccagc cctctggcgt
     6481 cataccagta aaaacgataa tgaacggtaa cgggttcttg cctttcgtta taaagcgttg
     6541 aggatgctga aggttgaata tcagacgtcg aaaggacggg cttttctgca ctgatgcccg
     6601 cagccaataa agatgactcc atcaccagcg attgctcatc attcaccgga atttccggat
     6661 gtgaacgaca gcccaccagc agtaacaacg ccagagacac cagcccaaag catccttttc
     6721 tcatcgttac agacctttat gcgccagcat tggtcccagc ggacggccac ccaacaagtg
     6781 catatggatg tggtaaacct cttgtccgcc atggcggttg gtattcatga tcagacgata
     6841 gccatcttcg gcaatacctt cttgctcagc aatttttgcc gctacggtga tcatgcgtcc
     6901 cagcgcctgc tcatgctcag ctgacacgtc gttcacagtc ggaatgagga tattcggaat
     6961 gatcagaata tgcgttggcg cttgcggcga aatatcgcga aacgccgtta ccagatcatc
     7021 ctggtagacg atatcggagg ggatctcacg acgaataatt ttgctgaata tagtttcttc
     7081 tgccacgaca ttttcctttt tcaaatagcc atgcgttgcg ccatgctacg ccgcgccaca
     7141 ctgcgagtat agagtatggt cgagttaaac ctgctctttc aaccttaact cacgatttct
     7201 taagcaaaaa atcgttttgc tgccgtatat atcgccatta ttcccatttc tgcctgcgaa
     7261 tgcgctgaaa agacattctc tgtttggttt acacgacaaa tatgaatgcg catatttctc
     7321 atttgcattt acaaacaaaa ttattcgcac actaaatata accgctcatc accctcctcc
     7381 ggcatgagcc tgacgacaac ataaaccaag agatttcaaa tgctttcaac acaatttaac
     7441 agggataatc aatatcaagc catcaccaaa ccgtcactac ttgccggttg catagcactg
     7501 gcactattac cttctgccgc ttttgctgca ccagccactg aagaaacggt gattgttgag
     7561 ggttcagcca cagctccaga tgatggcgaa aatgattaca gcgtaacgtc tacctctgcg
     7621 ggtaccaaaa tgcagatgac tcaacttgat attcctcagt cggtcactat tgttagccag
     7681 cagcggatgg aagatcagca gttacaaacg ctgggcgaag tgatggaaaa cacgctgggg
     7741 atcagcaaaa gtcaggcgga ttccgatcgt gctctttatt attcccgtgg attccagatc
     7801 gataactata tggttgatgg tatccccacc tattttgaat cgcgctggaa tctgggcgac
     7861 gcactttctg atatggcact ctttgaacgc gtagaagtag tgcgtggcgc gacaggactc
     7921 atgaccggga cgggtaatcc atctgcggca attaatatgg ttcgaaaaca cgcgaccagt
     7981 cgtgaattta aaggcgatgt ctcggcggaa tacggtagct ggaacaaaga acggtatgtg
     8041 gcggatttac aaagcccact caccgaagac ggtaaaatcc gcgcgcgaat tgtcggcggc
     8101 taccagaata acgactcatg gctggaccgc tacaacagtg aaaagacctt cttctcgggc
     8161 attgtcgatg ctgatttagg cgatcttacg acgctttcag ccggttacga atatcagcgc
     8221 attgatgtta atagcccaac ctggggcggt ttaccgcgct ggaatactga tggcagcagc
     8281 aacagttacg atcgtgcacg cagtaccgca cctgactggg cgtacaacga taaagagatc
     8341 aacaaggtct ttatgaccct gaagcagcgg tttgctgata cctggcaagc gacactgaat
     8401 gccacccact ctgaagtcga atttgacagc aaaatgatgt atgtcgatgc ctatgtaaac
     8461 aaagcggatg gtatgctggt tgggccatac agtaattatg gacctggctt tgattatgtc
     8521 ggcggcaccg gttggaacag tggcaaacgt aaagttgatg cgctggattt gttcgctgac
     8581 ggtagttatg aattgtttgg tcgtcagcac aatctaatgt ttggtggcag ttacagcaaa
     8641 caaaacaatc gttacttcag ttcatgggcc aacatcttcc cggatgaaat tggcagtttc
     8701 tacaacttta atggcaattt cccacaaacc gactggtcac cacagagcct ggcacaggac
     8761 gataccacac atatgaaatc gttatatgct gctactcgag tcacccttgc cgatccgctg
     8821 catctgatcc tcggcgcacg ttataccaac tggcgggttg atacgctgac ttacagcatg
     8881 gagaaaaacc acaccacgcc ttacgctggt ctggtgtttg acatcaatga caactggtcg
     8941 acctacgcca gctatacctc tattttccag ccgcaaaatg atcgtgacag ttcaggcaaa
     9001 tatctggctc caatcaccgg taacaactac gagctgggtc tgaaatcgga ctggatgaat
     9061 agccgtctga ccaccacgtt agccatcttc cgtattgagc aggataatgt cgctcagtcc
     9121 accggtacac ctatccccgg cagcaacggc gaaaccgcct ataaagcggt ggatgggaca
     9181 gtcagtaaag gggtggaatt tgaactcaac ggcgcaatta ccgacaactg gcagctgaca
     9241 tttggcgcaa cgcgctatat tgcagaggat aacgaaggaa acgccgttaa tcctaatctg
     9301 ccacgcacca cggttaaaat gttcaccagc tatcggttgc ctgtcatgcc agagttgaca
     9361 gtcggcggtg gtgttaactg gcaaaatcgc gtgtataccg acaccgtgac accgtatggc
     9421 accttccgcg ccgagcaagg tagctacgcg ctggtggatc tcttcacccg ctaccaggtg
     9481 acgaaaaact tctcgttaca ggggaacgtc aataacctgt tcgacaaaac ctacgatacc
     9541 aacatggaag gttctatcgt ctacggcgca ccgcgtaatt tcagcattac cggcacgtat
     9601 caattctgaa taacacctgt aaaaaaggca gccatctggc ggccttagtc tccccaacgt
     9661 cttacggatt agtggttacg gatgtactca tccatctcgg ttttcaggtt atcggattta
     9721 gtaccgaaaa tcgcctgaac accagaacca gcaaccacta cgcccgctgc gcccagtttc
     9781 ttcaggccag cctgatccac tttagacaca tcagcaacgc tgacgcgcag acgggtaata
     9841 catgcgtcga ggttagtaat gttttcttta ccaccaaatg cagcaaccag agccggtgcc
     9901 atttcgctgg tacctgttgc ttttgcatct tcagtcgcgt cttcacgacc cggcgttttc
     9961 agatccagtg ctttaatcag cacgcggaag atggtgtagt aaacaatcgc ataaccgata
    10021 ccgacgatcg ggaacagcca cagtttgctg ctgttaccag acagaacgat aaagtcgatc
    10081 agaccgtgcg agaacgacgt accgtcacgc atccccagaa gaatacagat tgggaatgcc
    10141 aggcctgcca gaatcgcgtg gatgatgtac aggatcggcg caacgaacat gaaggagaac
    10201 tcgatcggct cggtgatacc ggtcaggaac gaggtcagcg ccgcggagat cataataccg
    10261 cccactttcg cgcggttttc tggtttagca gagtgccaga tagcaattgc ggcagctggc
    10321 agaccgtaca ttttgaacag gaagccacca gacagtttac ccgcagtcgg gtcacccgcc
    10381 atataacgcg gaatgtcgcc gtggaaaacc tgacctgctg cgttggtgta ttcaccaatc
    10441 tgcatctgga aaggtacgtt ccagatgtgg tgcagaccaa acggcaccag gcaacgttcg
    10501 atgaaaccgt aaatgccaaa cgcaactacc gggttctggt aagcagccca ctgagagaag
    10561 gtctggattg cagaaccaat cggcggccaa atgaaggaca gcacaacgcc agtaaagatg
    10621 gcagccaggc cagaaatgat cggcacaaag cgtttaccgg caaagaagcc aagatactca
    10681 ggcagcttaa tacggtagaa acggttaaac atgtacgctg cgatcgcacc ggagataatc
    10741 cctccgagta cgccagtatc cgccaggtgt ttagaggcga tttcttcagc aggtaaatgc
    10801 agtaccagtg gcgcaaccac ggccatggtt ttaaccatga tgccataggc aacaactgcg
    10861 gccagcgcgg atacgccatc gttattggta aagccgaggg cgacaccgat cgcaaaaatc
    10921 agtggcatgt ttgcaaagac ggaaccgcct gcttctgcca taacatgcga tacaacggcg
    10981 ggcagccagc tgaaattcgc ggaaccgacg cccagcagaa tacctgcgat aggcagtacg
    11041 gataccggca gcatcagcga tttaccgacc ttttgcaggt tagcaaatgc attcttaaac
    11101 ataattgaga gtgctcctga gtatgggtgc tttttttacg ttctcacgcg tggcaagggg
    11161 ggagagcctc gccgtgtaca gggcatctaa gcgcccttta tttattacac agagtaaaat
    11221 aattcagtgc cgatttgttt gacggctatc acatttcaac ttcagatcat tggagaagtt
    11281 gcacattttg acaaaacata tgtcacaata tttcaaacgt ggttattcac cgcccaattt
    11341 tcggcggtga actttactcg ttttagccat taattacgag ctttaaaaaa actcattcaa
    11401 cggatggatt gaaggcggga agcgtcgatg tgaaacagac gggcgaagtt atcggtggtt
    11461 acctgcgcca gttcttcaac ggcaacacct ttcaacacag ccatgtattc tgcaacgtca
    11521 cgaaccatcg caggttgatt ctcttttcct cgatgcggta ccggcgcaag gtaaggtgag
    11581 tcagtttcca ccagtaaccg atccaggggg acataacgcg cagcatctcg aagttgctcc
    11641 gcattacgga aggtcacaat gccggaaaag gagatgtaaa atccgagatc cagtaattta
    11701 cccgccgttt ctctgtcctc tgtaaaacag tgtagtacgc caccgcaatc cgtcactttt
    11761 tcttcgcgca gaattgccag cgtatcggcg cgggcgtcac gggtatggac gataaccggc
    11821 ttgttcagtt cacgaccaat ctggatatga tggatgaagg actcttgctg acgaacttta
    11881 gtttccggtg tgtaataata atccagcccg gtttcaccca gcgctacaac accctcttct
    11941 gccgccagac ggcgtaaatc ttctacatcg tagggatcat tctggttgag tggatgcacg
    12001 ccacaagaaa atacgacgtt gtcacgttcg cctaccagat cccgcatatg taaataaccc
    12061 ggtaatgttg tggcgactgc cagacaaaat ttcacatcgc gtgcggcggc tttcgccaga
    12121 acgtcatcca cgtccttatg caaagattca taatccagac catcgagatg gcagtgtgag
    12181 tcgactaaaa acatgatgtc tctcttaaag atgaggaacc ggtagcacaa cgcccggttg
    12241 caggtaatgc tcaatacgca gtaaaagatc ggtgatgaga agctcgcggt tgatgcctgt
    12301 gacagacatt aactgttcac gaatgtggca aacatccccc agtatagcct gcaggcgcga
    12361 gggagaaaga tggtttgcca gttcgacgac aaggcccggc acatcaacat tggtcacctg
    12421 cgcagcacca tgatggcgtt ttagcgcatc catcagcaac gttgccagcc agtgtaaacg
    12481 cgccggagct tgttcatgat taagggccgc cagcagcgaa taccagtcgc ccgatggcac
    12541 gctatatgcc aatgcctgac acaatgtttc acgagcctgc cagttatctc cctgaaacaa
    12601 cgccagtgcc gcgccaggcg aaccggcgct taagcgcaat gcggcaagta atgcatcctg
    12661 tgacattgtc acttcgcgtg aaagccaggt cacggcgtac tgttccggcg gcggcgcaag
    12721 gtaatgtaac cgacaacgac tacgtaatgt tgccagtaaa cgttcaggct cgcgggtagc
    12781 caggaaaaac caggtttctg ctggtggctc ttcaagcgtt ttcagcaatg cgttagccgc
    12841 ggcgtcggtc agtaaggcag catcggttac ccagacaact ttcgcaccac ctaggcgtgc
    12901 gtgctcattc agcttttcgg tgacctcacg tactgcatca atgcccagcg tatttttccc
    12961 tttttcggga gccagggtgt agtaatcggg atgcgtgcca gcctgcatca actgacatcc
    13021 acgacagtga ccgcaacttt tgtggccctg cggttgttgg cagagtaaat aacggctcag
    13081 ggcgtagatt aaagcatcat cgcccatgcc cggtaacgcc tgaatgagta gcgcatggtg
    13141 acctcttccg gcctgatagc tggctaccag tttttcgaaa tcaggtcgta accatggata
    13201 ccatctcatg cgtccaactc cttcacccag tgggtcacgg tagtgcggat tgcatccatc
    13261 acggcctcca gcggctgggt ggcatcaatg gtatgaatac ttttatcttg tgcagccagt
    13321 tccagatagc gggcgcgggt gcgattaaag aaatcgaaag actcttgctc aatacgatcc
    13381 agctcgccgc gcgcacgcgc gcgttttaag ccaacttccg gggtaacatc gagatagagc
    13441 gttaagtcgg ggcgaaaatc cccgagaaca gcatcacgca gtgttgccag catatgttgg
    13501 tcaataccac gtcctccgcc ctgatacgcc tgagtggaga gatcgtggcg atcgccaatc
    13561 acccaggtgc cgttagccag agctggtttg atgaccgttt ctaccagttg aacgcgcgcg
    13621 gcataaaaca tcagaacttc ggctttatcg gtaatgactt catcgcctac cgatttgata
    13681 tccagcacca ggcttcttaa cttttcggca agttgcgtac cgccaggttc ccgagtgaaa
    13741 accatgtcgc ggatacccag ttgctcaagc gtctcaacca ccacattacg cgcggtagtt
    13801 ttgcctgcgc cttccagccc ctcaatgacg atatacttac tgcgcatttt tttccttaag
    13861 cactttcaga taatcctgca cagacttgtt atggctggca agattggtat taaacgtgtg
    13921 accaccttta ccatcggcca caaaatagag atacggcgtt tttgccggat gcgcagcagc
    13981 cttcagcgaa tccgcccccg gcgtagctat cgcacccggc ggcaaaccgg taatggtata
    14041 ggtgttatac gctgtcggcg tttccaggtc tgcacgagaa agtttgccat tataacgctc
    14101 tcccatcccg taaatcacgg ttgggtcggt ctgcaggcgc ataccaatgc gtaaacggtt
    14161 gataaatacc gaggcaacct gatcgcgttc actggcaacg gcggtttctt tttcgataat
    14221 tgatgccatc gtcaccagct gatttttatc tttataaggc agaccgtccg cacgcccttc
    14281 ccaggcgcta tcgaccgctt tcaccatttt cttgtgcgct cgcttgagta acgcgacatc
    14341 ggtggtattg gcggtataca tccaggtgtc tggccagaac caaccttcaa tccactccgg
    14401 gttttccagt tcaagtgcct gcgctacggt ggcgtactta tcgtcactca gcgtgtgctt
    14461 gatatacgga gcctcacgca attgcttgag gtagtcgctc agacgcatcc cttctaccag
    14521 tcgcagcggg aactgtgctt ctttaccgct ttccagcaat ttcagcatct cgcgcacggt
    14581 catctgcggt gtaaagcggt aagtcccggc tttaaagtga gaaagatccg gttcgatacg
    14641 cagcaaccat tgaaaaaccc gtgggcgatt gatgatctta tcggcataaa gctgttcacc
    14701 aagcgccaga cgtccggtcc ctggcttcag ggtaaatatc gtctcttctt tgataagcaa
    14761 tttgctgtcg gcaagatggc gaaccttcca gacgcccaca ccagcggcga tacccagtac
    14821 caccagcaat aacaagataa tcaataacac ttttttcatg actaattcgg gcgctcacaa
    14881 agtggggcta aatattcata taacgttgct gacgaaaagg agacatcgcc acaggcacgt
    14941 acgggcatca ctggcattaa cgcattacaa ataaccatct catctgcctg caacgcctct
    15001 tccagagagg cttgcacttc gacaagctga taagaggatt gtgccagcaa acggatacag
    15061 aattgtcgca taatgccgtt aacacctgcc tgatccagac gcggcgtata aactacattg
    15121 cccttccgcc agaacaaatt agccgcacag cattccgtaa cccacccttc gctgtcaagg
    15181 accagcgcct catcagcgtt tgtctgctca agatgagagc ggatcaatac ttgctcaagc
    15241 cgattaagat gtttaatacc tgcaagatga ggattgcgcc ccagccgcac cgggcttagc
    15301 gccaacgtca tcccttcgtt acgcaaacgg tcgtaatgtg caggataaac cgtaacggag
    15361 agaatccgcg ttgccggtcc actgttcaat gtgctgtacc ctcgcccgcc actaccgcga
    15421 ctgatcacga ctttcagcac accattttgc tgttctgctg ccagcgtttt catctcttgt
    15481 tcaagctgag gccagaagtc acagaaaatc atcaaccgct gacaagcatc ctgtagtcgc
    15541 tggatatgcg ccgataacaa actgacttta ccgtcgataa ctctggcggt ggtaaaacaa
    15601 ccatcaccaa actgcgttgc ccgatcgctt actgccagcg attcctgctt atgaccgtta
    15661 attaagaaca taccggctcc ttatcagaga attgttagtg tggcagcatg ttcactacgg
    15721 aacaagtcgg aataaaagct aagaaaaaag gcccgcaagc ggacctttta tatgggtggg
    15781 aaatgacaac ttagatcttt ttaaagatca aagaaccatt agtgccaccg aagccgaagg
    15841 agttacacag cgtgtattcc attccgctga cctgacgtgc ttcatgcggt acgaaatcca
    15901 gatcgcaacc ttcatccggg ttatccaggt tgatggttgg cggaacagcc tgatcgcgca
    15961 gcgccaggat ggagtagata gattctactg cacccgccgc acctaacagg tgaccggtca
    16021 tagatttcgt ggagcttacc aacacacggc ttgcagcttc accgaagatg gttttcaccg
    16081 cctgcgcttc agctttatcg ccagccggcg tagaagtacc gtgcgcgtta acgtagccaa
    16141 tctgactcgc ttcaatgcct gcatcacgca gagcatttgc catcgccaga gctgcgcctg
    16201 cgccattttc tggcggtgac gtcatatgat aagcatcgct gctcatacca aagccgacga
    16261 gttcagcgta aattttcgca ccgcgttttt tcgcgtgttc gtactcttca agtaccagca
    16321 taccggcacc atcgcccagt acgaagccat cacgctcttt atcccacggg cggctcgccg
    16381 cttgcgggtt atcattgcgg gtagataatg cacgtgccgc gccaaaacca ccaacaccca
    16441 gcggcgtact ggctttctct gcgccacctg caaccatcac gtcagcatcg ccatacgcga
    16501 taatacgcgc agcatggcca atgttgtgca cgccggaagt acaggcggtc gcgatagaga
    16561 tgcttgggcc acgcaggcca tacatgatag tcagatgacc tgccaccatg ttcacaatcg
    16621 ttgacggaac gaagaatggg ctgatcttac gtggaccacc gttcatcaga gatgtgtggt
    16681 tttcttcgat cagtccgagg ccgccaatcc cggagccaat tgcggcacca atgcgggttg
    16741 cgttctcttc cgttatatca aggccagaat cctgcatggc ctgaacgcca gcgacaattc
    16801 catattgaat gaaggcatcc atcttgcgct gttctttgcg cgagataatg tcctcacagt
    16861 taaaatcctt tactaagcca gcaaatttcg ttgcataggc gctagtatcg aaatggtcga
    16921 ttaggctgat gccactctga ccggcaagca gagctttcca ggtagactct acggtattgc
    16981 cgacaggaga caacatgccc agtccggtca caactacacg acgcttagac acgtttgtcc
    17041 tccagggagg gaaaaaatga ttctagtggg acaaaaagat aaaactcagg cggtcgaacg
    17101 accgcctgga gatgttcact tacgcctggt ggccgttgat gtaatcaatg gcagcctgaa
    17161 cggtggtgat tttctcagct tcttcgtccg gaatctcagt atcaaactct tcttccagag
    17221 ccattaccag ctcaacggtg tcaagagaat ccgcgcccag gtcttcaacg aaagaagcat
    17281 tgttggtaac ttcttcctgc ttaacgccca gctgttcgcc gataattttc ttaacgcgtt
    17341 cttcgatagt gctcatactc ttaaatttcc tatcaaaact cgctttcgcg atggttttcg
    17401 tagtgtataa aatgttgaaa aatttgcaac taaatcccgg caggtcttac cacgatttta
    17461 cgttattttg aggccttacc ccccaataac gcaaatcatt ttgcactaat tattactcat
    17521 aaccacgcag actgcgcgca acatgagcat tttgtgcaaa tcgcggtcag accatgtaca
    17581 tcccgccgtt cacatgcaaa gtttcacccg tgatgtaagc tgcttcgtcg gatgccagga
    17641 atgcaaccgc gttggcgatt tcctgtgcgc cgccgaggcg acccgcagga acctgcgcca
    17701 ggatacccgc acgctggtca tcgctcagcg cacgtgtcat gtccgtttca ataaagcccg
    17761 gagcaacaac gtttacagta ataccgcgtg acgcaacttc gcgcgccagt gatttactga
    17821 agccgatcaa gcccgctttc gccgcagcgt agttggcctg accgccattt cccatggtac
    17881 caaccacaga accgatagtg ataatacgac catgacgctt tttcatcata gcgcgcatta
    17941 ccgcttttga cagacggaaa acagatgaaa ggttggtttc gataatatcg ttccactctt
    18001 catctttcat tcgcattaac aggttatcac gagtgatacc ggcattattg accaggatat
    18061 ccacttcacc aaattctgcg cgaatttttt ccagaacaga ttcgatagat gccgggtcgg
    18121 tcacattcaa catcagacct ttgccgttgg cacctaaata atcgctgatc gcctgagcgc
    18181 cattttcact ggtcgcagtg ccaataactt tcgcgccacg ggctgcgagc gtttcagcaa
    18241 ttgcgcggcc aattccgcgg cttgcaccgg ttaccagtgc gatttttcct tcaaaattca
    18301 tgattttcct cttttaaagc tcaagcgccg ctgccatcgc tgaaggttcg ttcagcgccg
    18361 aggcggtcag ggtgtcgaca atgcgtttcg tcaggccagt aagcaccttg cccgggccga
    18421 cttcatagag atgttctacg ccttgcgctg ccatgtactc aacagacttc gtccactgaa
    18481 ccgggttata caactgacgt accagtgcgt cacggatggc atcaccattg gtttcgcatt
    18541 tcacatcaac gttattcaca acagaaactg ttggtgcgtt aaaggtgatt ttcgctaatt
    18601 ctactgccag tttgtcggct gctggtttca tcagcgcaca gtgagacggt acgctcactg
    18661 gtaacggcag agcacgtttt gcgcccgcag ctttacaggc agcgccagca cgctcaaccg
    18721 cttctttatg accggcaata accacctgtc ccggagagtt aaagtttacc ggagaaacga
    18781 cctgacctgc tgcagcttct tcacacgctt tcgcaataga cgcatcatcc agaccgatga
    18841 ttgccgccat agcgcccgta ccttccggta cggcttcttg catgagcttg ccgcgcatct
    18901 caaccagacg caccgcatca gcgaaatcaa tcacaccagc gcaaaccagc gcggaatatt
    18961 cccccaggct gtgaccggcc atcattgccg gtgctttacc gccctgctgc tgccatacgc
    19021 ggtacagcgc aacagaggca gtcaacagcg ctggctgggt ttgccaggtt ttattcagtt
    19081 cttcagctgg cccctgctgg gtcagcgccc acaggtcgta gcccagcgcc gcagaagctt
    19141 cagcaaacgt ttcttcgaca attggatagc tcgccgccat atcagccagc attccaacgg
    19201 tttgagaacc ctgtccaggg aacacaaatg caaattgcgt catgttttaa tccttatcct
    19261 agaaacgaac cagcgcggag ccccaggtga atccaccgcc aaaggcttca agcagaacca
    19321 actgccccgg cttaatgcgc ccgtcgcgta cagcttcatc cagcgcgcac gggacagagg
    19381 ccgcagaggt attaccgtgg cgatccagcg tcaccacgac attgtccata gacataccga
    19441 gttttttcgc cgttgcactg ataatacgca ggttagcctg atgcggaacc agccagtcca
    19501 gttgagaacg gtcaagatta tttgccgcca gcgtctcatc aacgatgtgc gccagttccg
    19561 ttaccgcaac cttgaagact tcgttgcccg ccatcgtcag atgaattgaa ttctctggat
    19621 tcacacggtc agcattaggc agcgtcagca actcaccata gctaccgtcg gcatgcagat
    19681 gggtggagat gattcccggc tcttcagagg cagccagcac cgcagcgccc gcgccatcgc
    19741 caaaaataat aatagtccca cgatcggttg gatcgcaggt gcgcgccagt acatcggaac
    19801 cgacgaccag agcatacttc accgccccag atttcacgta ttgatcggct acgcttaatg
    19861 cataggtgaa acctgcgcag gctgctgcaa cgtcaaatgc cgggcaacct ttaatgccca
    19921 gcatgctttg aatctgacaa gctgcgctcg ggaaagcgtg cgtagcagaa gtcgttgcca
    19981 caacgatcag gccaatctgg tctttctcaa tgcccgccat ctcaattgcg cgtgtcgccg
    20041 cttcaaagcc catggttgaa acggtttcgt ttggcgcggc aatgtggcgt tcgcggatac
    20101 cggtacgagt gacaatccac tcgtcagagg tgtccaccat tttttccaaa tcggcgtttg
    20161 tccgcacttg ttcgggcaga tagctgccag taccaataat cttcgtatac atgtacgctc
    20221 agtcactttt cggttatata ccgtcacttg caaactgcga gttcgctggc agcgtcctgc
    20281 taccgcagag ttccgctttt gccaccgtcc agcagctcaa aaccagctgg gtatacagat
    20341 tccaggcgag cggcaattcg ctgaggaact tgtcgctgca ccgcctgcac tgcctgttca
    20401 atcgcgaccg caaaagctcg ctgattggct gcaccatgac tttttatcac cgtgccgcgc
    20461 aatcctaaca gacaggcgcc gttatactgg tcggggttga ggtgactgaa tcgcctcgtc
    20521 aggctctttt gtagccaacg ctttaataac agtagccacc acgaccgttt tttcccttca
    20581 ccctgagatt tcagcagaga aaggaacatc ctgacaacac cttccatcgt ctttaatgtg
    20641 acatttcctg taaagccgtc acaaaccagc acatctgtct tgccagttaa caactcattg
    20701 gcttcaagat agccgatata attgatagaa gggattgttt taagcaccgc tgaggcatcc
    20761 cgaatactgt cgagaccctt tacttcttct tcaccaatat tgagcaacgc cacgcgagga
    20821 ttgggaattt ccaccacctc ttcagccaga actgagccca taatggcaaa ttgcaccaac
    20881 attgtactgt cacaatcgac gttggcccct aagtcaagga ccaccgtttt gcccttttgc
    20941 tgatgtggta ataccgtcac cagcgccgga cgctcaatcc cctccagggg cttgagtaat
    21001 aattttgcca gccccatcag tgccccggta ttaccggcac tgacacaggc ttgcgctcga
    21061 ccttctttca ccagctccag ggccacgcgc attgaactcc cacgactggc gcggatagct
    21121 tgcgaaggcc gggcatcact ggcgataact gactgcgcag gaataatctg cagacgcgaa
    21181 cgttgttcaa agtcagcttt agcaagtaat ggcgtgatgg cgtcgggatt gccgactaaa
    21241 agaagagtga gttgcgaatt agaattcagt gcctgcaatg ctgcaggcac tgtcacggaa
    21301 gggccaaaat cccctcccat gacatctaac gccagggtta gacgtgtcaa ggtatcgtcg
    21361 ccgcccggtt tggtctttcc ccagttgcct ggggaaaatc ctcactaagc ttcatcacgc
    21421 agacgcgtga ttacttagcg atgaccttgc ggccgcggta gtaaccgtcg gcagtgatgt
    21481 ggtgacgcag gtgtttttca ccagaagttt tgtctacaga caggctggtg actgcggtca
    21541 gcgcgtcatg ggaacgacgc atgccacgtt tggaacgggt tggtttattc tgttgtacgg
    21601 ccatggacct tactcctcaa ttacggttta tccccgatcc aacggggagc accaattact
    21661 tacgctttaa gctggctaat acggcaaatg ggtttggctt ttgcacttct tcaggcagtt
    21721 caccaaagac catgtccgct tcggacactt cacagtgttc agaatcatgc accggaacta
    21781 ccggcaaggc gaggatgatt tcatcttcaa ccattgcaag cagatcgatt tcaccgaatt
    21841 cgttaacctc aatcggttca tacgcttccg gcagtgcttc agcctgttcg tctgaacgca
    21901 caggactaaa acaatacgtt gtgtagacct gatgagtaaa cggcttcccg caacgctgac
    21961 actcgagcgt taccgtcacc ttcgcatcgc cgtttaacac tgcgagacgt tggttatcga
    22021 tagcgaacga catggagcat tccacatcac tgtccacact gactacggat tcggcgacgc
    22081 gctcaacctg atcaggggta tagataccct ggtaatcaag gcgtttttga gccgtacgaa
    22141 ccggatcgag agtcaggggt aattttacct tttgcatagg gcgcgcatat taactttgta
    22201 acgtcataga gtcaaagaaa aaggcagcct gtggttgcct tttgccaata attcgcacac
    22261 attgcgggtt actgctttat tttcgttcag tgctgataac gaacatcaac ctgcaaaacg
    22321 aagcgcatag tttaaaatgg ctatcatcaa tacgctatat ctgatggaaa aaaatatgcc
    22381 taaacttatt ttagcctcca cctcgccatg gcgtcgcgct cttctggaaa aactgcaaat
    22441 ctcttttgaa tgtgcagcac cagaggtcga cgaaacccca cgcagcgacg aatcaccgcg
    22501 acagttggtg cttcgactgg cacaagaaaa agcgcaatct ctggcgtcac gttatccgga
    22561 tcatttaatt attggttcag accaggtatg tgttcttgat ggcgaaatca ccggtaaacc
    22621 gttaacggaa gaaaatgccc gtctgcaatt acgcaaagcc agcggcaata tcgtcacctt
    22681 ctatactgga ctggcgttgt ttaattcggc gaatgggcat ctacaaacag aagtggagcc
    22741 ttttgacgtc catttccgtc acctgagcga ggcggagatt gataattacg tgcgtaagga
    22801 gcatcccctg cactgcgcgg gtagctttaa gagtgaagga tttggcatta cgctgtttga
    22861 gcgtttagag ggacgtgacc ctaacacgct ggttggttta ccgctaatcg cgctgtgtca
    22921 gatgttacgt cgggaaggga aaaacccgct gatgggatag ttgtttgcgc tggatgcggt
    22981 tacgtcgcat ccggcaatcg gtgcaggcgg cctgatgcgg cgtaaacgcc ttatcaggcc
    23041 aaccgcccat tatcaggctt atatgcttgt attagcgcgc gttacgcagc ttttgcaaac
    23101 aacgcttcaa accttcatcc atcggcgctt cgatacgcat cacctcaccg gtccccggat
    23161 gggtaaactt caacgctgcg gcgtgcagga acagacgatt taatcccgtg cctgcttcag
    23221 tgagctgtct gtcaaattca cggtcaccgt agcgatcgtc aaaggcaatc ggatgacccg
    23281 catactgtgt atgcacacgg atctgatgag tacgtccggt taccgggcta caacgcacca
    23341 gggtggcaaa tgcatagcgt tcttccactt taaagcgtgt ttccgacggt ttgccttcct
    23401 gactgacacg cacgatacgt tcgccgcttt gcagaatatt tttcaataac ggcgcttgaa
    23461 cgctcttcac atgcgactgc cactgaccgc gcaccagcgc caggtaatct ttttgcattc
    23521 ctttttcacg taattgctca tgcagagaac gcaacgccga gcgttttttc gctatcagca
    23581 acacacctga agtgtcccgg tcgagacgat gaaccagttc aaggaaccgc gcttccgggc
    23641 gcaacgcccg caaaccttcg ataacgccga agcttaaacc actgccgcca tgtaccgccg
    23701 taccggaagg tttattcagc accaggatat gatcatcttc atataagatg acgtccgcca
    23761 gcgccgccac cttttgcaga tgtggcgaaa ccgcctcttc ttcccgctca gcaacgcgaa
    23821 ccggtgggat acgcacctca tcacccgctt cgagtttata ttcaggctta atacgttttt
    23881 tgttcacccg cacttcgcct ttacgcaaaa tacggtaaat catacttttt ggtacgcctt
    23941 tcaattgggt acgcaaaaag ttatcgatac gttgccccgc ttcgtcagcg gtgatagcaa
    24001 caatttttac ggatggagtc tctgttttca tggtgggcga ttctaattag ccaacaggat
    24061 tcgcgccact catttttcta tgcttatatt tagttttgca ccttattact tcactacgtg
    24121 atcactttat tgatggttat taaaccaatc accagcaaga agtgaaaaaa ctgtgagtaa
    24181 gcgggtgata aatggtaaaa gtcatcttgc tataacaagg cttgcagtgg aataatgagg
    24241 ccgtttccgt gtccatcctt gttaaaacaa gaaattttac ggaataaccc attttgcccg
    24301 accgatcatc cacgcagcaa tggcgtaaga cgtattgatc tttcaggcag ttagcgggct
    24361 gcgggttgca gtccttaccg gtagatggaa atatttctgg agagtaatac ccagtctgtt
    24421 tctttgataa ttgcgctgtt tttccgcatg aaaaacgggc aaccgacact ctgcgcctct
    24481 ttgagctgac gataaccgtg aggttggcga cgcgactaga cacgaggcca tcggttcaca
    24541 cccggaaagg cgttactttg cccgcagctt agtcgtcaat gtaagaataa tgagtaagtt
    24601 acgatgaaaa gaatgttaat caacgcaact cagcaggaag agttgcgcgt tgcccttgta
    24661 gatgggcagc gtctttatga cctggatatc gaaagtccag ggcacgagca gaaaaaggca
    24721 aacatctaca aaggtaaaat cacccgcatt gaaccgagtc tggaagctgc ttttgttgat
    24781 tacggcgctg aacgtcacgg tttcctccca ctaaaagaaa ttgcccgcga atatttccct
    24841 gctaactaca gtgctcatgg tcgtcccaac attaaagatg tgttgcgtga aggtcaggaa
    24901 gtcattgttc agatcgataa agaagagcgc ggcaacaaag gcgcggcatt aaccaccttt
    24961 atcagtctgg cgggtagcta tctggttctg atgccaaata acccgcgcgc aggtggcatt
    25021 tctcgccgta tcgaaggcga tgaccgtacc gaattaaaag aagcactggc aagccttgaa
    25081 ctgccggaag gcatggggct tatcgtgcgc accgctggcg taggcaaatc tgctgaggcg
    25141 ctgcaatggg atttaagctt ccgcctgaaa cactgggaag ccatcaaaaa agccgctgaa
    25201 agccgcccgg ctccgttcct gattcatcag gagagcaacg taatcgttcg cgcattccgc
    25261 gattacttac gtcaggacat cggcgaaatc cttatcgata acccgaaagt gctcgaactg
    25321 gcacgtcagc atatcgctgc attaggtcgc ccggatttca gcagcaaaat caaactgtat
    25381 accggcgaga tcccgctgtt cagccactac cagattgagt cgcagatcga gtccgccttc
    25441 cagcgtgaag tccgtctgcc gtccggtggt tcgattgtta tcgacagcac cgaagcgtta
    25501 acggccatcg acatcaactc cgcacgcgcg acccgcggcg gcgatatcga agaaaccgcg
    25561 tttaacacca acctcgaagc tgccgatgag attgctcgtc agctgcgcct gcgtgacctc
    25621 ggcggcctga ttgttatcga cttcatcgac atgacgcctg tacgccacca gcgtgcggta
    25681 gaaaaccgtc tgcgtgaagc ggtgcgtcag gaccgtgcgc gtattcaaat cagccatatt
    25741 tctcgctttg gcctgctgga aatgtcccgc cagcgcctga gcccatcact gggtgaatcc
    25801 agccatcacg tctgcccgcg ctgctccggt accggtaccg tgcgtgacaa cgaatcgctg
    25861 tcgctctcta ttctgcgttt gatcgaagaa gaagcgctga aagagaacac ccaggaagtt
    25921 cacgccattg ttcctgtgcc aatcgcttct tatctgctga atgaaaaacg ttctgcggtg
    25981 aatgcgattg aaacgcgtca ggacggtgtt cgctgcgtga ttgtgccaaa cgatcagatg
    26041 gaaaccccgc actaccacgt gttgcgcgtg cgcaaagggg aagaaacgcc gaccttaagc
    26101 tacatgctgc cgaagctgca tgaagaagcg atggcgctgc cgtctgaaga agagttcgct
    26161 gaacgtaagc gtcctgaaca acctgcactg gcaacctttg ccatgccgga tgtgccgcca
    26221 gcgccaaccc cagctgaacc tgccgcgact gtcgtagccc cagcaccgaa agctgcaacg
    26281 gcaacaccag cagctcctgc acaacctggg ctgttaagcc gcttcttcgg cgcactgaaa
    26341 gcgctgttca gcggtggtga agaagccaaa ccgaccgagc aaccaacacc gaaagcagaa
    26401 gcgaaaccgg aacgtcaaca ggatcgtcgc aaacctcgtc agagcaaccg ccgtgaccgt
    26461 aatgagcgcc gcgacacccg tagtgaacgt actgaaggca gcgataatcg cgaagaaaac
    26521 cgtcgtaatc gtcgccaggc acagcagcag actgccgaga cgcgtgagag ccgtcagcag
    26581 gctgaggtaa cggaaaaagc acgtaccacc gacgagcagc aagcgccgcg tcgtgaacgt
    26641 agccgccgcc gtaacgatga taaacgtcag gcgcaacaag aagcgaaggc gctgaatgtt
    26701 gaagagcaat ctgttcagga aaccgaacag gaagaacgtg tacgtccggt tcagccgcgt
    26761 cgtaaacagc gtcagctcaa tcagaaagtg cgttacgagc aaagcgtagc cgaagaagcg
    26821 gtagtcgcac cggtggttga agaaactgcc gctgccgaac caattgttca ggaagctcca
    26881 gctccacgca cagaactggt gaaagtcccg ctgccagtcg tagcgcaaac tgcaccagaa
    26941 cagcaagaag agaacaatgc tgataaccgt gacaacggtg gcatgccgcg tcgttctcgc
    27001 cgctcgcctc gtcacctgcg cgtgagtggt cagcgtcgtc gtcgctatcg tgacgagcgt
    27061 tatccaaccc agtcgccaat gccgttgacc gtagcgtgcg cgtctccgga actggcctct
    27121 ggcaaagtct ggatccgcta tccaattgta cgtccgcaag atgtacaagt tgaagagcag
    27181 cgcgaacagg aagaagtaca agtgcagccg atggtgactg gggtccctgt cgccgccgct
    27241 gtcgaacctg ttgttagcgc accggttgtt gaagaagtgg ctgaagttgt agaagccccc
    27301 gttcagattg ccgaaccgca accggaagtg gttgaaacga cgcatcctga agtgattgcc
    27361 gccgcagtaa ctgaacagcc gcaggtgatt accgagtctg atgttgccgt agcccaggaa
    27421 gttgcagaac acgcagaacc ggtggttgaa ccgcaggaag agacggcaga tattgaagaa
    27481 gttgccgaaa ctgctgaggt tgtggttgct gaacctgaag ttgttgctca acctgccgcg
    27541 ccagtcgtcg ctgaagtcgc agcagaagtt gaaacggtag ctgcggttga acctgaggtc
    27601 accgttgagc ataaccacgc tacggtgccg atgacgcgcg ctccggcacc ggaatatgtt
    27661 ccggatgcac cgcgtcacag tgactggcag cgccctactt ttgccttcga aggtaaaggt
    27721 gccgcaggtg gtcatacggc aacacatcat gcctctgccg ctcctgcgcg tccgcaacct
    27781 gttgagtaat aattagctca agtaatcaag ccctggtaac tgccagggct tttttatttc
    27841 atctttgaat caacatatta aaattcatcg aatggcatcc ttgctaacca acaatgcaaa
    27901 ataggcaaaa aaatgcccgc cccggcgatc ccagagcagg cagacaaaac atacccagtt
    27961 tcatgatatg ttttaaagcg aaattatttg ttgagctgga agagcgacaa tccctgcata
    28021 tcggtaaatg ctttatacga tgcctgcaat gccgtttgct gcatgatgta agatgaaata
    28081 gttgcattcc agtccacatc aaccagatcg ctcatctgct gcgtttgccc taaagcgcga
    28141 tcgctaccta atgaatccag cgactccagt tcgttcagct gcgtgcctaa ttccgcgcga
    28201 acggtcagca cattgttcag cgagtttttc agtccgcggt tggttttatc taacgccgca
    28261 gcggcggttt ctttatccgc ttcgctatcc gcgaccggcg ttttcagggc tgcgatggcg
    28321 ctatccagca tggcaaaaag attggtttca gaagcgctac cgtctggttc cgctaccgcg
    28381 ttactggtaa tactgtcgaa aattttgtcg cccgtatgtc ctatcaccat cgaacgcgaa
    28441 gcatcgacct gttgcttaat actttctgtt ccgccgacat aatcaccgtt agcctcacta
    28501 aacggcgcag tctctgtttt ataaccggca aaaatgtagc gcccattacc gtcagtggtg
    28561 ttcgccagat tcagcaactg gtcacgaagc ccctgaatat ccgtagccag cgaggcccgg
    28621 tcatcgtcac tcaaggtgcc attgctggcg tagacaattt tttcctgagc attctggata
    28681 gcagtggtga cctggctaag tacactctct tccagtgaca ctttttgagt ggcgaaagta
    28741 cgcgccagcg tgtactggct gttttgcgcc tgtgcctggg agagaacaac cgcttgtgat
    28801 gcagcaatgg gatcatctga agggttaacg actcgcttac ccgtcgacat ctgttcgccg
    28861 tacttcatcc attctgcctg agaattggtg ataccacgca tgttttgctg gtacatcatc
    28921 tgggtactga aacgcatttt atctcccctt agcgaatgtt aatcagcgca tcaaaaatcg
    28981 cgtttgccgt ctgcagaacc tgcgcattcg ccaggtaata ctgctgaaaa cgttgcagat
    29041 ttccgtactc ctcatcgaga ttgacaccgg aaatcgactg ctgctgattg gaaagctgcg
    29101 tcaccacatt accttgcgtg gtgctactgg ttttcaacgt cgcggtttta ttaccgatat
    29161 cactcactaa cgaggcataa gcgtcgttaa aggatttcgc accgcccacc gttttactat
    29221 tgctttgcag atccagcagg gcctgaccgt tgcggttatc gctatcaccc gcatcttttt
    29281 cgctcgccat cgctattttc gcttcgtcgg tgattaatac atccatgttg acgatggcgt
    29341 cacttactgg tttcagcgtg aagctgtcgt taacggcagg cgttcctgta aacgtcaact
    29401 ccagaccatc aaatgccact ttaccgttgg catccggcgt caccgtaaaa gtggtattgc
    29461 tggcaaggcg ggtgacctgc cactgattat tatcgaacga gattttgtaa tctgtcgcca
    29521 gtaccacgga ggcatcagtt accgtggcac cgatcgcaac gtcacctttg tttttcgtgt
    29581 tttgcagaac cgcgggctta ccgatagtaa agaaatcttc accagcatcg ccgttagcat
    29641 caaatccggc tttgtgttgg gtgttgaaag cctcggcaaa tgccagcgcc agttgtccaa
    29701 gcgtattacg cgtctggtcc agctcctgag aacggaatgt cagaatgccg cccagcgacc
    29761 cggtattcag taatttctcc gggatctcaa tattgcctgc cgtcccatca acataagcga
    29821 cagtcgtacg agaagggtca gcgctggaag gaactgccgc cagttgccgc gccgtacttc
    29881 cctgaaccag tgagtaacca ttggccaccg tgatgttata agtgccgcca tcctgaacgc
    29941 tgacttctac accaacaatc tggtttaatt cgctcaccag ttgatcgcgt tgatccagca
    30001 gattgttagg tgacgcccct gcccccacgc ctgtcaggcg cgagatttga tcgttcaggc
    30061 tggcaatttg tttagcgtaa ttgttgatct gatcaacgct ggcacctatc gcgatattga
    30121 cctgtttgtc ctggtcgcgc agatattgat cggtggtttt aaactgattc accaaccctt
    30181 ctgatttccc aatcagcgcc tggcgcgctg ccgggtcttc cgcgttactc accagcgttt
    30241 gcaggctggt gaagaaatcc tgcatctgtg ttgccagcga agaggtactg gtggagagca
    30301 tattgtcgat tttcgacatc tgctcatagc gggcggtcag gccgctactt tgcgtctgcg
    30361 cagcacgtaa ctggttggta ataaacgcat catactcacg ctgcacacca gaaacgtaga
    30421 cgccattgcc aacccagccg ccagcgccca acgtgctatt ggcctgcgcc ataatagtgg
    30481 tttggcgggt atatccggca acgttatagc tggagatatt attacttgcc gtatttaacg
    30541 ccgcctgggc cgcgttcagt ccgctcatgg cgttattgat caagctggac atggaggttc
    30601 cttataagcc ttcaattaca gagtattatc ggcagcgacc cgccggactt gagttattca
    30661 gaacagatta tcaatgttca tgctgtaggt tttgctcacc ttgtcgctta tcgatttcat
    30721 ctgctggatc atgttggtga gtttacgggc atagtgagga tcggtagcat accccgcgtc
    30781 ctgtagggcc tgcgccccct gctccgcact cgcggctgtc gtcacggcgg cgtagcgcgg
    30841 gttacgtgtt aacagcccaa cgtaatccga caatgcttcc agatacgagc tgtagacccg
    30901 aaatttcgct tttactttct tcgcttcgcc attttcatat tcagtcgtgg tgatttcagt
    30961 aactggacct ttccagttgc cagaggcttt gacgccaaac aggttatagc tcggctcgcc
    31021 gttttcgcgg cggatttgcc gttgccccca accagactcc agcgccgcct gagcgaggat
    31081 caaatgatgt ggcacaccgc tttgctggct tgccagttgg gcgggcagcg agagttgcgc
    31141 gaggaatgct ttactgtcac ccggcagcga atcatcgtag ttacgtggca cggccttttg
    31201 caccagctgc gaaagcgcct gattttgata acgcaccaca gtttcgagcg ggaatttcat
    31261 cggtgctgct ggcgtggact cctctggcaa tggttgttct ggcgtcatct gtttaaccat
    31321 catctctgca agccccagac ctttgcccgc cgtcatctgt tgggcaatct gctggtcata
    31381 catactggta tacaggcgag tgtgctcgct gctgaacagg ccatcttttg gtaaagcgtc
    31441 gcgcatgctt ttcaacatca tctgcacgaa catcccttcc acctgacggg ccaccggacg
    31501 gatatttgcc gccggatctt cgcccgcttt cgcctttagt tcgttgagtg attgcgcatc
    31561 ccaggccgca cttgccagta gtttgctgtc gctgatcatc agatgatttc cagttttgcc
    31621 cgcagacatc ccgcactttg cattgattgc agtatggaca tcagatccat cggcgtagcg
    31681 cccagcgcat tgagcgcgcg caccacgtta ttgaggctgg cgctggaacg tacgctttgc
    31741 agcgaaccgc cgctctggcg taaatcgatc tgcgtttgtg gagtaaccac agtctgtcca
    31801 ccaccaaacg gtgtatctgg ctggctgaca ttggcctgac gattgactgt tactgagaga
    31861 tttccctgcg ctaccgcgca gctgtcgagg gtcacttcgc gattcatcac caccgaaccg
    31921 gtgcgcgagt taatcactac tttagcgtcc tgcggggtga cattaacctg catattctgg
    31981 atatctgcaa ggaaacggac ctgggaactg ttgccacttg gtacgcgcac ctgaatagtc
    32041 cgcgcatcta acgcggtggc gctgccatat ccacgcacgc ggttgatggt gtcagcgatt
    32101 tgctgcgcca tgctgaaatc ttcgtcgtta agttgcaaat taagggtatt cccgacgcca
    32161 aactggctgg gcaattcacg ttcaataacc gcaccattgg tgatccgtcc accgttcagt
    32221 tggttcacct gaacactgct accgccagcg gaggctcctg cgccgccaac cagaatattg
    32281 ccctgcgcca gcgcatacac ctgactgtca acgcccttaa gcggcgtcat caacaacgta
    32341 cctccacgca agcttttggc atttcccatg gaagaaacca ccacatcgat ggtttgcccc
    32401 tgacgtccaa acggaggaag tgactctgtc accattaccg cagcgacgtt ttttagctgc
    32461 atattggtgc ccgtcggaac ggtaattccc agctgtgaga gcatgttatt aagcgtttgt
    32521 gtggtaaacg gcgtctgggt tgtctggtca ccggtgccat ccagccccac caccagacca
    32581 tagccaatca gtgagttttg ccttaccccc tgaacactgg tgagatcgcg aatacgctca
    32641 gcctgagccg ccgtcgtaac cagtagaaga attaatgcag agagaaattt aatcaccaca
    32701 gcctcactta cattggcgac aggttaagga agaaacgctg caaccagccc atattttgcg
    32761 cttcgttaat gtagccattg cctacgtatt caatgcgcgc atccgccacc tgagtagacg
    32821 gtacggtatt gctgccgctg atagtgcgtg gattaaccac gccagagaag cgaataaatt
    32881 cggtaccctg attaatggca atctgttttt cacccaccac atgcaggttg ccgttgacca
    32941 gtacctggtc aaccgtcacc gtcaacgtgc cgctaaaggt attgctggca ttggccccgc
    33001 cctttccgtt gaacgtgtta ccaccggagg cttcgacatc ggcacgagcg ttaccaaaca
    33061 gcccctgcaa atagcgcggc acagtatcaa agccaaaatt agttttaccg tcacggctgg
    33121 cattcgcaga ggagcttttg ctggcgctga cgttctcctg caacacgatg gtcagcgtat
    33181 cgccaatatt gcgtggtcga cgatcttcaa acagcggttg atagccatag ttaatcggct
    33241 gagcagactg gaaaatagaa ccgttggcga cgggcgtcgg accgggaacc ggttgtgcac
    33301 tggtcgcccc ctgcaccagc ggcgtggagg gtatccaggc gcagccggtt agtgaaagca
    33361 ccaacaagct ggaaatggca taagtatgcg cagcgttttt ttgcatggct atcatcttca
    33421 aaaatcagta aaccggtgaa cctgccaccg gttaagcctt agagttgcgt cagtttttgc
    33481 agcatctgat cggtggtgga caccgcttta ctgttgattt cgtaagcgcg ttgcacctga
    33541 atcatattga ccagttcttc cgccacgttg acgttagacg tttcaacata cccttgatac
    33601 agcagtcccg cgccgttcag gcccggcgtg ctttcgttcg gtacaccaga ggattgcgtt
    33661 tcggtgtaga ggttttcgcc aatgctctcc agcccggtgt cattcataaa ggtggtgaga
    33721 ttgagctgcc caacctgaac cggagctgcc tggccttgtt gggttacgct gaccacgcca
    33781 tcacgaccga tggtgatact taacgcattc gccggaatgg tgatcgctgg ctgcacctga
    33841 aaaccaccag ctgcccgttc tgatccacct ggaaagagcc gtcacgggta taggctgatg
    33901 aaccatctgg caacatcacc tggaaaaagc cctgcccttt aatcgcgaca tctttgctgt
    33961 tgttggtctg cgacaggttt ccctggctgt gtaagcgttc agtggcgacc gggcgtaccc
    34021 ccgtgccgat ttgtaatccg gagggtaagg tggtttgttc ggaagactgt gcccccggct
    34081 ggcgaatggt ttgataaagc agatcttcaa acaccgcgcg ctgacgctta aaaccgttag
    34141 tactgacgtt tgccaggttg ttggcaatga cgtccatatt ggtttgctgg gcgtcaaggc
    34201 ccgttttggc gatccataat gaactgatca taaaatgtcc tgtagtggat tagtgacaaa
    34261 gtgcatatgt cttcttgccg gatgcggcgt aaacgcctta tccggcctac aagtgctcgc
    34321 aaaatcgata aattgcgatt gcccccatag acccgataaa cttgcgcatc gggcaactta
    34381 tacttgtcat gtatcctttc aattaactca tcgacagcag ttggttggca cggcctgcgt
    34441 tatcatcgac gctgctgatc accttcatct gcatttcaaa acgccgcgcg ctggcaatca
    34501 tgtcgctcat tgccgcaacg gcattgacgt tactgccttc cagaaccccc gacatcacac
    34561 gcaaggttgg atctgcctgc agtaccggcc ctcgcgtggc ctgggtttct gcgcttaaac
    34621 gaaaaatgcc gtcgtcaccg cgctgcactt cgctgcccgt ggctttcacc agtttcagac
    34681 gccctactgg cgcaactgta tttgccggat cgcccggatt gagcgccgag attgtgccat
    34741 cggcagcaat agtgatttcc gctccttccg gcacagcaat tggccccgcc tcgcctatca
    34801 cctgatgccc ctgaattgtc agttgcccgg tgggatcaac ctgaatgctg ccattacgcg
    34861 tatacccttc gctgccgtca gcggtctgca cggccagcca gccatcctgc tgcaacgcga
    34921 cgtccagcgg gcgcgaggtg taatccattt tgccgggcgt catatctgcg cccggcgttg
    34981 acgccgtgac caacgtgcgc gtgggcagag aaagcccttc cactggcacc gcgcgtaaag
    35041 cattcaactg cgcgcgaaaa ccgggcgttg aggcattggc cagattactg gcggttaccg
    35101 cctgttgatt cagtgtctgg ctggctgctc ccatcgcggt ataaattgcg tgatccattg
    35161 agctatcccg tcagcgatta gcgtaagtta accagcgtgt tgaggatctg gtcctgagtt
    35221 ttgatggtct gggcgttaga ctgatagtta cgctgggcaa cgatcatatt gaccagttct
    35281 ttactgagat cgacattgga cgcttccaac gcaccgttgg tcagggtgcc aaagttgccc
    35341 gtcccggctg tccccaacag cgccacgcca gaagattgcg tcgcagacca gacgttgtcg
    35401 ccttcggatg ccagaccttc gttgttggca aagttcgcca gtacaatctg ccccagcagt
    35461 tgtgtttgtt cgttggaata gttgccgaca accgtaccgt catcattgat ttgataactc
    35521 accagatcgc ccggtttgta gccattctgg gtggttgcca caatattgtt agcgccggta
    35581 ttttgctgca tggagttgag gaagctcagg ctaaacgtgg cgggatctgc gccgttaatt
    35641 gcgccagtgg taatattctc tgttggattt gaggtcagaa cgccattggc attaaacacc
    35701 agcttcattg caggctcggc tgtacctgtt ggatcactgc tatcctgggt gtagacctgc
    35761 cagttattat ccccggtctt cacaaagtag acgctcatgt catgagcatt accctgactg
    35821 tcgaaaacag tcaccgaacc ttttttgtta tagctatccg cattgctggc gctgaatggc
    35881 gtaacagtag gaagcggatc actggaattc aggttgatct gcatcgacgc cgtggtggta
    35941 gttttcgctg ccatcagggt attcgggatc gaaatattgg tcggattcgc cccttgctga
    36001 atagtcggcg gcgtaccggt tgccgggtaa cccgtcagct gtaaaccttg catattcacc
    36061 aggttacggt tttcatccag cttaaattgt ccgttacggc tgtagaacac cgaaccgttg
    36121 ctgtctacca gacggaaaaa accgttctgg ctgatagcaa cgtccagacc tcgcccggtg
    36181 ttggtggtcg tgccatcggt aaagtcctga gtgataccgg caacttttac ccccagtccc
    36241 actttcgaac cggcaaacat atcggcaaaa gaggccgtgc ctgatttaaa gccgtaggtg
    36301 gcggagttgg cgatattgtt gccaataaca tcgaggttgg tggcggcagc gtttaatccg
    36361 ctaaccgctt gagaaaaggc catgactgac tcctgaaatg tgaaggctta aattatctgc
    36421 cgtacttcgt cgagggtggt ggtgccgtaa gtgccgagat ccagcgtatt accgctgttg
    36481 ccgcggagca caccctgcac cagagcaaac tgcagcggct gggcaaccag ttgtgtacca
    36541 ccgttactgg cgctaatcgc tacattgtaa gaaccgttcg gcgcagtgct gccatcagtc
    36601 aacgtaccgt cccaggtgaa actgtggact ccggcggtca gttcaccaat atcaatggtg
    36661 cgcacaaccg cgccattttt atcggtgatg gtggccgtaa ctttgtctgc cgcctgttgc
    36721 agctcaacac caaacggcgt ggtcgtggtc acagcccctt cttcactgcc ggttcccgca
    36781 agaacagtgg tgccggggat catcacgccg tgaccgatca ggttactggc ctgtaacgac
    36841 tggctgttat caatctgtcc ggaaatagat ccgagcgtgg tattgagttt ttcaatcccg
    36901 ctgaccgtgc tgatttgtgc caattgcgac gtcagctcgt tgttttccat tggattggtc
    36961 gggtcctggt ttttcagctg cgccaccaac aaagtcagaa aactgctttg taagtctgcg
    37021 gcgttgctgc ccgtgagcga actactgctg gtggtactga cgccggtatt tgtcggatcg
    37081 gtggtggtta ccgcaatgga catagctttc tcctttattg accgagcgta agggttttca
    37141 gcatcatgct tttcaccgtg ttgagcactt caacattggc ctgatagctg cgtgacgccg
    37201 acatggtgtt aaccatctcc ccgacgacat caacgttcgg catttttacg tagccctttg
    37261 catccgccag cggattaccc ggttcataaa ccagtttgtc cggggcctga ctttctataa
    37321 catcggcaac ctttacgccg cctgtcgcag cacctggtgc agcgttaacc tggaatacca
    37381 cctgttttgc ccgatatggc tgtccatcgg gaccggtcac gctatcagca ttcgccagat
    37441 tactggccgc cacgttcaag cgctgggact gggcagttaa cgccgacccg gcgatatcaa
    37501 aaatattcag cagtgccatc cgttaatttc cgctctgtaa aacgttcatc atgcctttga
    37561 tttgcccgct caacgcgcta aggctcatct ggtattgcag gctgttatcg gcaaactggg
    37621 tgcgttcgcg atccatatcg acggtattac cgtcaagcga aggctggtcc ggaatacggt
    37681 attgcagttc tgcggtagga ggcgtcagcg cctgcgccgg aatgtgttgc gttgaggtca
    37741 tcgtcagtgc aaccacactg gttgcatccc gtccacgttg catgactttt ttaagttcac
    37801 tggcaaaatc gatatcgcgc gcctgataac caggggtatc ggcattggcg atgtttgctg
    37861 ccagcacttc ctgacgctgg gcgcgcagat tgagcgcctc ttgttgaaaa cgtaaggcgg
    37921 cgtcgagctt atcgagcata tctcctccgc aggtatcaaa attctgccat cagcttaaat
    37981 gcctttacgc gcgcgttatc ggcggaataa acgcaaaatg ggtcgctatt tatgccgttg
    38041 atgaccattg cggacaggta caattcacgt tgtagaaatg gctgggggcg aaaatgctgg
    38101 caataaaacg tagcgtggcg atcatctcga tactattcac tccgttaagt gcggcgagta
    38161 atctcacatc gcaattgcaa acctttttta gcgcccagct cgcgggggta agtgatgagg
    38221 ttcgtgtttc tattcgtaca gcgcccaatc tactaccgcc gtgcgagcag ccattgcttt
    38281 cgatgagcaa taattcccgc ctgtggggca atgtgaatgt gttggcacgc tgcggtaacg
    38341 acaaacgata tttacaggtt aatgtacagg ccacaggaaa ttatgtggtt gccgcgatgc
    38401 ccattgtgcg gggaggaaag ctggaagctg gcaatgtcaa actaaaacgc ggacggcagg
    38461 ataccctgcc accgcgtacg gtgctggata tcaatcaact tgttgatgcc gttagcctgc
    38521 gcgatctatc acccgatcaa cctatccagt taacccagtt tcgccaggca tggcgggtaa
    38581 aagcgggaca acgcgtcaac gtgatcgcca gcggtgacgg gtttagcgcc aacgcagaag
    38641 gtcaggcgct gaacaatgca gccgtcacac agaatgcgcg ggtgcgcatg gtatcgggac
    38701 tggtggtcag cggcgttgtt gatgcagatg ggaatattct tataaaccag taagctgtta
    38761 aagattaccc gtcccttgcc gataaataag caacacatga taaaagcgcc ctcaatgagg
    38821 aataaaccat gagtattgat cgcacttcgc ctctgaagcc cgtaagcacc gttcaaccac
    38881 gcgaaaccac tgacgcgtcg gtaacgaaca cccgggcggc aaaaacaaca gcctccacca
    38941 gcaccagtgt gacgttaagc gacgcgcaag caaaactgat gcaacccggc agcagtgata
    39001 tcaatcttga acgtgtcgaa gcgttaaaac tggcgattcg taacggtgaa ctaaaaatgg
    39061 acaccggcaa aattgccgat gcgctgctca acgaagcgca gcaagacttg cagagtaact
    39121 gatcgtatga cacgtcttgc agagatcctc gaccagatgt ccgctgtgct taacgatctc
    39181 aaaacggtaa tggatcaaga acagcaacat ctctctatgg ggcagatcaa cggcagccag
    39241 ttgcaatgga ttacagaaca aaaaagctca ctgctggcga cgctggatta cctcgaacag
    39301 ttacgcagga aagaacccaa tacagcaaat agcgttgata ttagtcaacg ctggcaggaa
    39361 attactgtga aaacgcagca actacgccaa atgaatcaac ataacggctg gttactggaa
    39421 ggacagattg agcgcaatca acaggcgctg gaaatgttga aaccgcatca ggaaccgacg
    39481 ctatatgggg cgaacggtca gacctcaaca acccatcgcg gcggtaaaaa gatttcgatc
    39541 tgaatattat ctccggcctg cactgcaggc cggaatgcat tgttacaccg tccggcgggc
    39601 aaattcttta actttgaagc ccagtaccgc cagtgcagcg aagtacgcgg caatccccgc
    39661 cagcacgacc gccattaaac gcagtaagcg ccagggcatg gtacccaatg accactccgg
    39721 catgatatgt aacataccta aaagcacgcc agacatcacc agtaccgcca ccaccagacg
    39781 caacagaaac gccatccagc cgggttgcgg agtaaagatt ttctgcttac gcaactgcca
    39841 gtaaagcagc gaagcattca gacacgccgc cagaccaata gaaagtgaca gcccagcatg
    39901 tttcaacgga ccaataaacg ccaggttcat caattgcgtt aaaatcagcg taacgatggc
    39961 aattttcact ggcgttttaa tgtcctggcg ggaataaaag ccaggagcca acactttcac
    40021 tacaatcagg ccaatcaaac ccaccgagta ggcaattaac gcccgctggg tcatcagcgc
    40081 atcaaacgcg gtaaatttac cgtactggaa cagcgaaacg gttaacggac ccgaaagaat
    40141 gcccaacgca accgcactcg gcagcgccaa caggaaacaa agacgcaacc cccagtccat
    40201 caaacggttg tattcatcat gattgccact ggcaaaactt ttcgacagcg acggcagcaa
    40261 aatggtgcca agcgccacgc ccagcacacc ggagggaaac tccattaagc ggtcggcgta
    40321 atacatccaa gacaccgaac cggaagcaag aaacgaggca aaaatggtgt tgatgattaa
    40381 ggagatctgg ctcacagaga cgccaaggat cgccggtccc atctgtttca ccacgcgcat
    40441 tgctccggca tcgtggaagt taatgcgcgg caggaccagc atgccgatct tcttcaggtg
    40501 cggtagctga tacaccagct gcaggatgcc gccgaccgta acagcccacg ccagcgccag
    40561 caccggtggg ttaaagtacg gtgcggcaaa cagcgcgaaa ccaatcatgc tgatgttaag
    40621 cagtgttggc gcaaacgccg gaatcgagaa gcggttccac gtattcagaa tcgctcccac
    40681 cagcgacgcc agggagatca gcaagatata gggaaaggta atctttagta gctggctggt
    40741 cagggcaaat ttgtcagctg tgtcagcgaa gcctggcgcg gtcaccatga tcacccacgg
    40801 tgcggcgagc atgccagcga ccgtcacaac cgccagcgca agtgtcagca ggccagaaac
    40861 ataagagaca aagacccgcg tggcgtcttc accctgctta cttttatatt ccgccagaat
    40921 cggtacaaat gcctgggaaa atgccccttc ggcaaagata cggcgtaaca agttaggaag
    40981 tttaaaagcg acgaaaaagg cgtcggttgc catccctgcg ccaaagattc tggcgacaat
    41041 tgcgtctcgt gcgaagccaa gcacacgcga aaacatggtc atcgagctga cggcggccag
    41101 cgattttaat aaattcatcg gtgttctaat ccagacccac gggcaaaacg cctgcaaggc
    41161 aggcgctgaa agaggcgaat agtctacctg gattatggtg aattgctacc gccagatgtt
    41221 acagggttta ttcactcatc gcatcgcgcc agatcttgtc aacgatacgt tgcgccagca
    41281 ctgcctgttc gccggcggtt tgcagaactg tctgattttg cacacattca atgaagtgcc
    41341 gcgcacagcc gacaaatcca cgttgctcaa gagtgctctg ccaaccagga atcggtttat
    41401 gcactacgcc ctgcccgcgc tcctcacgcc attcgcgcat atccgtaatg tcgatgagcg
    41461 catcgtcagt cacggcctgc acggtttcac gctgacttcc ggcacggcga tgcatacagg
    41521 tggtgatctg caaaggatcg gccgaaaaat ggtgctcggc aaacagcatt tcgccagcgt
    41581 cgttagtcag tagcgtaccg ccatccagag aggctttgcc gcccgacaac cacagcgcgg
    41641 tatccaccac atgcagataa tcatccagca acgtgaaata aagatcgtgt ggcccgacac
    41701 tattgctacg atgtttatcc attcttagcg aagctgcggt agcgagttgc gtttttaact
    41761 caccgtagag tggtgcgaaa cgacggttaa aaccgaccat caacgtcagt tttttccgcg
    41821 ccgccagttc caccagccgt tcagcatcgc gcagattttc tgccagcggt ttatcgacac
    41881 agacatgtac acccgcattg agtaacgtac tgaccacgtc aaagtggctg gcggtgctgg
    41941 aatgcacaaa aaccgcatcg caactggcgg caaggctgga taacgaatcg gcataaggaa
    42001 tgcgccagct ttcacaaatt ggcagggctt tcgcgcgcgt aggcgaccag gctccttgta
    42061 acatccagtc agacgctgcc gccagcaccg gtaaccacgc tttttgcgca atgccgccta
    42121 atcccacaac gccgatacgt aattttttca ctttaatctc ccagatgggc cagcaacgaa
    42181 tcaagacgct gtttcagttc tgccacttcg atttccaggg cttcgacgcg ggcctgtaaa
    42241 tcaccatcaa ccgcgttcga catatccgtt accgccggct gatcctctac ctcaccactg
    42301 aaaagatgca tgtagcggct ttcgcgttta cccggttcgc gggccagacg caccacaaaa
    42361 ggaccatctt cgcgatttgc cagttgttcc agcgtcgact ccacttccgc catatcgctg
    42421 aattcataca ttcgcgcggc gcggctgcgc agttcacccg gcgtctgggc accacgcaat
    42481 aacaacgtgg tgattaacgc cacttccgct gcgctcagtt tcagatcgcc aaattctgaa
    42541 ttacaaaaac gttgttcata tttggtgacc cgattaccaa agccactcac cgtgcgcaga
    42601 taatggcgtt tgaccagatt atccagttgc tcctgcactt cagattcact cagattcatg
    42661 accggttcac ggttcgtttt ctgattacag gccgtgacta caccattgac tgagagcggg
    42721 tattgctccg gcgtggtcac ctgcttttcc agcaagcaac caatcacgcg agcttcaagg
    42781 gcagtaagtt gatatttcat gagattctcc ttagcggccg ggcgtccagt ctggggtagt
    42841 taatgccgtc agtacgtgat cgcgccattg tccatcaatc aacagatagt ctttcgcata
    42901 gccttctttt tcaaaaccca gtcgcgccag taaatcaccg ctgcgtttat tgtgcggcat
    42961 ataattagcc ataatgcgat gaatatgttg ggtgcgctgc atataacgaa tggctgcggt
    43021 cagggcttca aacatgagtc ctttgccctg ccatttttgc ccaatcgaat aaccgagata
    43081 gcaggcatga aaagagccac gaacaacatt ggaaaaattg gcaacgccaa taatctcttt
    43141 ttcgtccggg tcgaataagc caaagtagaa agctgaacct tgtttatgaa attcgttaat
    43201 catccccagc ctggcctgcc agcctgatgg ataacagtgg ctttcgtcgc gcactggctc
    43261 ccagggcttg aggaaatggc gattctctgc gtaataatcc gcaagacgcc aggcatcacg
    43321 atcatgcacc agacgcacga ccagtcggtc tgtggttaag cgcacttttg gcacgttact
    43381 gcgatagcca aacattctat acctactcct tcccgtaacg tctttaatac gctgtaagta
    43441 ttactatacc tgcgcaacca gaccctgtga aaagtccaaa atgccatttt ctgcattatt
    43501 cacatatttg ataaaaactc tctatcaaag cgatgttttg ataaaaaaat attgtccccg
    43561 aagtgatggg aaaagtctta gcggcaatgc agaatagaaa gtgctcatct tttatttccc
    43621 gggaggggaa atgtcccgcg tatcgcaggc gaggaacctg ggtaaatatt tcctgctcat
    43681 cgataatatg ctggtcgtgc tggggttctt tgttgtcttc ccgctgatct ctatccgctt
    43741 cgttgatcaa atgggctggg ccgccgtcat ggtcggtatt gctctcggtc tacgccagtt
    43801 tattcagcaa ggtctgggta ttttcggcgg cgcaattgcc gaccgctttg gtgccaaacc
    43861 gatgattgtt accggtatgc tgatgcgcgc cgccggattc gccacaatgg gtatcgccca
    43921 cgaaccgtgg ctattgtggt tttcatgcct gctctcggga ctcggcggca cgttgtttga
    43981 tccgccgcgt tcggcgctgg tggtgaaact aatccgtcca caacaacgtt gtcgtttttt
    44041 ctcgctgttg atgatgcagg acagtgccgg tgcggtcatt ggcgcattgt tggggagctg
    44101 gctgttgcaa tacgactttc gcctggtctg cgccacaggg gccgttttgt ttgtgctgtg
    44161 tgcggcgttc aatgcgtggt tgttaccggc atggaaactc tccaccgtac gcacgcccgt
    44221 tcgcgaaggc atgacccgcg tgatgcgtga caagcgtttt gtcacctatg tcctgacgct
    44281 ggcgggttac tacatgctgg ctgtacaagt gatgctgatg ctgccaatta tggtcaacga
    44341 cgtggctggc gcgccctctg ccgttaaatg gatgtatgcc attgaagcgt gtctgtcgtt
    44401 aacgttgctc taccctatcg cccgctggag tgaaaagcat tttcgtctgg aacaccggtt
    44461 gatggctggg ctgttgataa tgtcattaag catgatgccg gtgggcatgg tcagcggtct
    44521 gcaacaactt ttcaccctga tttgtctgtt ttatatcggg tcgatcattg ccgagcctgc
    44581 gcgtgaaacc ttaagtgctt tgctggcgga cgcaagagct cgcggcagct atatggggtt
    44641 tagccgtctg ggtctggcga ttggcggcgc tattggttat atcggtggtg gctggctgtt
    44701 tgacctgggc aaatcggcgc accagccaga gcttccgtgg atgatgctgg gaattattgg
    44761 catcttcact ttccttgcgc tgggttggca gtttagtcag aaacgcgcaa cgcgtcgttt
    44821 gctggaacgc gacgcctgat ttgatactcc cccctgactc ctccatactg aaagtgaact
    44881 gacagccaaa tggaggagtc acgtgaagct atacatttac gatcactgcc cttactgcct
    44941 caaagcccgc atgattttcg gcctgaaaaa tatccccgtc gaattacatg ttctgctcaa
    45001 cgacgacgca gaaacaccca cccggatggt cggtcaaaaa caggttccca ttctgcaaaa
    45061 agatgacagc cgctatatgc cagaaagcat ggatatcgtt cactatgtcg ataaactcga
    45121 cggcaaaccg ttactgaccg gcaaacgttc ccctgccatt gaagagtggc tgcgcaaggt
    45181 caatggctac gccaacaaac tgctgttgcc gcgttttgcc aaatcggcat ttgatgagtt
    45241 ttctactccc gccgcgcgca aatatttcgt cgacaagaaa gaggccagcg cgggtaattt
    45301 tgccgacctg ctggcccact ctgacggtct gattaagaat atcagcgatg atttgcgtgc
    45361 gctggacaaa ctgatcgtca aaccgaacgc cgtgaatggc gaactttcgg aagatgatat
    45421 tcagctattt ccgctactgc gtaatctgac gctggtagcc ggaatcaact ggccaagccg
    45481 cgttgctgat taccgcgata atatggcgaa acagacacaa atcaatttgt tatcatcaat
    45541 ggcgatttaa tcctgacccg ccgcggcagc ctgtattcgc gggttgccgc cgttttcagt
    45601 tttttctgct ggcgagttta tcgctggtga cacatgctgc gttgtgtcag gatattgacg
    45661 cagatttcat gtcgaggact accatgaaca agtttttatt tgccgctgca ttgatcgtca
    45721 gtggcctgct cgttggctgt aatcaactca cccaatacac catcaccgaa caagaaatta
    45781 accagtcgct tgcgaaacat aataatttct caaaagatat cggtttaccc ggcgtggctg
    45841 acgcccatat tgttctgaca aacttgatca gccaaattgg tcgcgaagag ccgaataagg
    45901 ttaccctaac cggagacgcc aatctggaca tgaactccct gttcggtagt cagaaagcga
    45961 ccatgaaact gaagctgaaa gcgctgcccg tgtttgataa agaaaaaggt gcgatcttcc
    46021 tgaaagagat ggaagtggtc gatgcgacgg tacaaccgga aaaaatgcaa acggtgatgc
    46081 aaacgttgct tccctatttg aaccaggcat tacgcaatta ctttaaccag caacctgctt
    46141 acgtcctgcg cgaagatggc agtcagggcg aagcaatggc gaaaaaactg gcgaaaggca
    46201 ttgaagtgaa gccaggcgaa attgtcattc cgtttacgga ttaatgacga gggcgcattc
    46261 gcgcccttta tttttcgtgc aaaggaaaac gtttccgctt atcctttgtg tccggcaaaa
    46321 acattccttc agccggagca tagagattaa tgactgcacc atcccaggta ttaaagatcc
    46381 gccgcccaga cgactggcac cttcacctcc gcgatggcga catgttaaaa actgtcgtgc
    46441 cgtataccag cgaaatttat ggacgggcta tcgtaatgcc caatctggct ccgcccgtga
    46501 ccacagttga ggctgccgtg gcgtatcgcc aacgtattct tgacgccgta cctgccgggc
    46561 acgatttcac cccattgatg acctgttatt taacagattc gctggatcct aatgagctgg
    46621 agcgcggatt taatgaaggc gtgttcaccg ctgcaaaact ttacccggca aacgcaacca
    46681 ctaactccag ccacggcgtg acgtcaattg acgcaatcat gccggtactt gagcgcatgg
    46741 aaaaaatcgg tatgccgcta ctggtgcatg gtgaagtgac acatgcagat atcgacattt
    46801 ttgatcgtga agcgcgcttt atagaaagcg tgatggaacc tctgcgccag cgcctgactg
    46861 cgctgaaagt cgtttttgag cacatcacca ccaaagatgc tgccgactat gtccgtgacg
    46921 gaaatgaacg gctggctgcc accatcactc cgcagcatct gatgtttaac cgcaaccata
    46981 tgctggttgg tggcgtgcgt ccgcacctgt attgtttacc catcctcaaa cgcaatattc
    47041 accaacaggc attgcgtgaa ctggtcgcca gcggttttaa tcgagtattc ctcggtacgg
    47101 attctgcgcc acatgcacgt catcgcaaag agagcagttg cggctgcgcg ggctgcttca
    47161 acgccccaac cgcgctgggc agttacgcta ccgtctttga agagatgaat gctctgcagc
    47221 actttgaagc attctgttct gtaaacggcc cgcagttcta tgggttgccg gtcaacgaca
    47281 cattcatcga actggtacgt gaagagcaac aggttgctga aagcatcgca ctgactgatg
    47341 acaccctggt gccattcctc gccggggaaa cggtacgctg gtccgttaaa caataaaaat
    47401 cgtagcttcc tgttgtcatt aggttattta acctgtataa ataaccagta tattcaacag
    47461 ggggctatta tgcgaattga agtcaccata gcgaaaactt ctccattgcc agctggggct
    47521 attgacgccc tggctggcga actttcccgc cgtattcagt atgcgtttcc tgataatgaa
    47581 ggccacgtat cggtacgtta tgccgcagcg aataatttat cggttattgg cgcaacaaaa
    47641 gaagataaac agcgcattag cgaaattctc caggaaacgt gggaaagcgc cgatgactgg
    47701 tttgtcagcg aataatatgc agtgattttt gtttttgccg ggtcgccccg gcttttttat
    47761 tttacttacg aattttgagt acaaaatttc tacacttcct caaaataatt gcacaatatg
    47821 ctaaaaaaat atgaatgaca tgctgtttta ttgttcatat aaatcttaaa agcagaaatt
    47881 tatctgttcc cccctttaaa aattcggtga atacccttac ttattggtat actgaagttg
    47941 cttcagataa aaacgcattg aacctcgaat aacgttgtct agtaacacga attagggggc
    48001 catgatggaa aaaaataatg aagtcattca gactcatccg ctcgtagggt gggacatcag
    48061 caccgttgat agctatgatg cgctgatgtt gcgtttgcac taccagaccc caaataagtc
    48121 cgagcaggaa gggactgaag ttggtcaaac gctctggtta accactgatg ttgccagaca
    48181 atttatttcg atattagaag caggaatcgc caaaattgaa tccggtgatt tccaggtaaa
    48241 cgagtatcgg cgtcattaac atgacatgac catccaccca aaaggcacct caccggtgcc
    48301 tttaccattt ctgggttgta tatctgcatt cagttaatta ctctgcttaa accatgagag
    48361 cagagagaaa aaaatgaaat acgatctcat cattattggc agcggttccg taggcgctgc
    48421 cgccgggtat tatgcaaccc gcgccggttt aaacgtgcta atgaccgacg cccatatgcc
    48481 accgcatcaa cacggcagcc accacggcga tacgcgatta attcgccatg cttatggtga
    48541 aggcgaaaag tatgtcccgc tggtcctccg cgcgcaaacg ctgtgggatg aactttcccg
    48601 ccacaacgaa gatgatccca tttttgtacg ctctggtgtc attaacctcg gcccggctga
    48661 ctccgcattt ctcgccaacg tcgcccacag cgccgaacag tggcaactca acgttgaaaa
    48721 gctcgatgcg caagggatta tggcccgctg gccagaaata cgcgtcccgg acaactacat
    48781 cggcttattt gagactgatt ccggtttttt gcgcagcgaa ctggcgatta aaacctggat
    48841 ccaactggcg aaggaagcgg gctgtgcgca actgttcaac tgcccggtca ccgcaattcg
    48901 tcatgacgat gatggcgtaa ctattgaaac ggttgacggt gagtatcagg cgaaaaaagc
    48961 gattgtctgc gcgggaacat gggtaaaaga cctgctcccg gagctgcctg tccagcccgt
    49021 acgtaaagta tttgcctggt atcaggccga tggccgctat agcgtgaaga ataaattccc
    49081 ggcgtttacc ggtgaactgc ccaatggcga tcaatattat ggttttccgg cagaaaacga
    49141 cgcgttgaag attggcaaac ataacggagg ccaggttatc cattcagcgg atgaacgtgt
    49201 tccgtttgcg gaagtggtca gcgatggttc ggaagccttc ccgttcttgc gcaatgtatt
    49261 gccgggtatc ggttgctgcc tgtacggcgc tgcctgcacc tatgataatt cgcctgacga
    49321 agattttatt atcgataccc tacccggcca cgataataca ctgctcatta ccggcctgag
    49381 tgggcacggt tttaaatttg cgtcagtttt aggggaaata gctgccgatt ttgcgcaaga
    49441 caaaaaaagc gattttgatt tgacgccatt caggctttcc cgcttccaat aatcatcatt
    49501 acggcctcca gaatgaggcc gtttttacag gagtatttaa tgcgtcggct gttgcactat
    49561 ctca
//
LOCUS       DADMKF010000009        46747 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.009, whole genome shotgun sequence.
ACCESSION   DADMKF010000009 DADMKF010000000
VERSION     DADMKF010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 46747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46747
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.009"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(17..868)
                     /gene="pdxK"
                     /locus_tag="JNC24_000502"
                     /gene_synonym="thiJ"
     CDS             complement(17..868)
                     /gene="pdxK"
                     /locus_tag="JNC24_000502"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HAY9157345.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            973..1341
                     /locus_tag="JNC24_000503"
     CDS             973..1341
                     /locus_tag="JNC24_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HAY9157346.1"
                     /translation="MKKIICLVITLLMTLPAYAKLTVHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAQ"
     gene            complement(1344..2255)
                     /gene="cysM"
                     /locus_tag="JNC24_000504"
     CDS             complement(1344..2255)
                     /gene="cysM"
                     /locus_tag="JNC24_000504"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HAY9157347.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNASLVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(2389..3486)
                     /gene="cysA"
                     /locus_tag="JNC24_000505"
     CDS             complement(2389..3486)
                     /gene="cysA"
                     /locus_tag="JNC24_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HAY9157348.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNIAFGLTVL
                     PRRDRSNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPEQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQRGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            complement(3476..4351)
                     /gene="cysW"
                     /locus_tag="JNC24_000506"
     CDS             complement(3476..4351)
                     /gene="cysW"
                     /locus_tag="JNC24_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HAY9157349.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            complement(4351..5184)
                     /gene="cysT"
                     /locus_tag="JNC24_000507"
     CDS             complement(4351..5184)
                     /gene="cysT"
                     /locus_tag="JNC24_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HAY9157350.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLAQMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            complement(5184..6200)
                     /locus_tag="JNC24_000508"
     CDS             complement(5184..6200)
                     /locus_tag="JNC24_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY9157351.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWTKENGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQTRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRN"
     gene            complement(6371..7162)
                     /gene="ucpA"
                     /locus_tag="JNC24_000509"
     CDS             complement(6371..7162)
                     /gene="ucpA"
                     /locus_tag="JNC24_000509"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HAY9157352.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DDDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPL
                     RRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            complement(7291..8148)
                     /gene="murR"
                     /locus_tag="JNC24_000510"
     CDS             complement(7291..8148)
                     /gene="murR"
                     /locus_tag="JNC24_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HAY9157353.1"
                     /translation="MLYLTKIRNAESEFTENEQKIADFLRANVSELKSVSSRKMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIEVISKAPFIQITGLGGSALVGRDLSFKLMKI
                     GYRVAYEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQLNDVESLKMIQR
                     SSELTQRLK"
     gene            8312..9208
                     /gene="murQ"
                     /locus_tag="JNC24_000511"
     CDS             8312..9208
                     /gene="murQ"
                     /locus_tag="JNC24_000511"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAY9157354.1"
                     /translation="MQLEKMITEGSNTASAEIDRVSTLEMCRIINDEDKTVPLAVERV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGTEDSREGGINDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            9212..10450
                     /gene="murP"
                     /locus_tag="JNC24_000512"
                     /pseudo
     CDS             9212..10450
                     /gene="murP"
                     /locus_tag="JNC24_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040446.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
     gene            10463..10636
                     /locus_tag="JNC24_000513"
     CDS             10463..10636
                     /locus_tag="JNC24_000513"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylglucosamine transporter subunit
                     IIBC"
                     /protein_id="HAY9157355.1"
                     /translation="MGLNSAFGPSGLVALPLMTSAQGILPAMAVYAGGILVAWVCGFI
                     FTTLFGCRNVNLD"
     gene            10641..11945
                     /gene="pbp4b"
                     /locus_tag="JNC24_000514"
     CDS             10641..11945
                     /gene="pbp4b"
                     /locus_tag="JNC24_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin binding protein PBP4B"
                     /protein_id="HAY9157356.1"
                     /translation="MKRTMLYLSLLAVSCSVSAAKYPVLTESSPEKAGFNVERLNQMD
                     RWISQQIDVGYPGVNLLIIKDNQIVYRKAWGAAKKYDGSVLMEQPVKATTGTLYDLAS
                     NTKMYATNFALQKLMSEGKLHPDDRIAKYIPGFADSPNDTIKGKNTLRISDLLHHSGG
                     FPADPQYPNKAVAGALYSQDKGQTLEMIKRTPLEYQPGSKHIYSDVDYMLLGFIVESV
                     TGQPLDRYVEESIYRPLGLTHTVFNPLLKGFKPQQIAATELNGNTRDGVIHFPNIRTS
                     TLWGQVHDEKAFYSMGGVSGHAGLFSNTGDIAVLMQTMLNGGGYGDVQLFSAETVKMF
                     TTSSKEDATFGLGWRVNGNATMTPTFGTLASPQTYGHTGWTGTVTVIDPVNHMAIVML
                     SNKPHSPVADPQKNPNMFESGQLPIATYGWVVDQVYAALKQK"
     gene            complement(12003..12902)
                     /gene="yfeX"
                     /locus_tag="JNC24_000515"
     CDS             complement(12003..12902)
                     /gene="yfeX"
                     /locus_tag="JNC24_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HAY9157357.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(12998..13573)
                     /locus_tag="JNC24_000516"
     CDS             complement(12998..13573)
                     /locus_tag="JNC24_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HAY9157358.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWRGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            complement(13634..14083)
                     /locus_tag="JNC24_000517"
     CDS             complement(13634..14083)
                     /locus_tag="JNC24_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HAY9157359.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRATFPRTWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            complement(14070..14495)
                     /locus_tag="JNC24_000518"
     CDS             complement(14070..14495)
                     /locus_tag="JNC24_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase YpeA"
                     /protein_id="HAY9157360.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            14709..15578
                     /gene="amiA"
                     /locus_tag="JNC24_000519"
     CDS             14709..15578
                     /gene="amiA"
                     /locus_tag="JNC24_000519"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HAY9157361.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            15582..16481
                     /gene="hemF"
                     /locus_tag="JNC24_000520"
     CDS             15582..16481
                     /gene="hemF"
                     /locus_tag="JNC24_000520"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HAY9157362.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCLPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(16487..17539)
                     /gene="eutR"
                     /locus_tag="JNC24_000521"
     CDS             complement(16487..17539)
                     /gene="eutR"
                     /locus_tag="JNC24_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
                     /protein_id="HAY9157363.1"
                     /translation="MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHA
                     LTITAWQQIYDQLHPGKFHGEFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPA
                     TRGEQGFIGSQCLGSAEIATRPGGTEFELSTPDDYTILGVVLSEDVITRQANFLHNPD
                     RVLHMLRSQSALEVKEQHKAALWGFVQQALATFCENPENLHQPAVRKVLGDNLLMAMG
                     AMLEDAQPMVTAESISHQSYRRLLSRAREYVLENMSELVTVLDLCNQLHVSRRTLQNA
                     FHAILGIGPNAWLKRIRLNAVRRELISPWSQSTTVKDAAMQWGFWHLGQFATDYQQLF
                     AEKPSLTLHQRMREWG"
     gene            complement(17585..18085)
                     /gene="eutK"
                     /locus_tag="JNC24_000522"
     CDS             complement(17585..18085)
                     /gene="eutK"
                     /locus_tag="JNC24_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HAY9157364.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVKEPD
                     APVIVAESADELLALLTSVRQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            complement(18098..18757)
                     /gene="eutL"
                     /locus_tag="JNC24_000523"
     CDS             complement(18098..18757)
                     /gene="eutL"
                     /locus_tag="JNC24_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HAY9157365.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            complement(18767..19654)
                     /locus_tag="JNC24_000524"
     CDS             complement(18767..19654)
                     /locus_tag="JNC24_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutC"
                     /protein_id="HAY9157366.1"
                     /translation="MDQKQIEEIVRSVMASMGQAAPAPSEAKCATTNCAAPVTSESCA
                     LDLGSAEAKAWIGVENPHRADVLTELRRSTVARVCTGRTGPRPRTQALLRFLADHSRS
                     KDTVLKEVPEEWVKAQGLLEVRSEISDKNLYLTRPDMGRRLCAEAVEALKAQCVANPD
                     VQVVISDGLSTDAITVNYEEILPPLMAGLKQAGLKVGTPFFVRYGRVKIEDQIGEILG
                     AKVVILLVGERPGLGQSESLSCYAVYSPRMATTVEADRTCISNIHQGGTPPVEAAAVI
                     VDLAKRMLEQKASGINMTR"
     gene            complement(19675..21036)
                     /locus_tag="JNC24_000525"
                     /pseudo
     CDS             complement(19675..21036)
                     /locus_tag="JNC24_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311339.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutB"
     gene            complement(21048..22451)
                     /gene="eutA"
                     /locus_tag="JNC24_000526"
     CDS             complement(21048..22451)
                     /gene="eutA"
                     /locus_tag="JNC24_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HAY9157367.1"
                     /translation="MNTRQLLSVGIDIGTTTTQVIFSHLELVNRAAVSQVPRYEFIKR
                     EISWQSPVFFTPVDKQGGLKEAELKTLILEQYQAAGIAPESVDSGAIIITGESAKTRN
                     ARPAVMVLSQSLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANY
                     ALFDAGKISGTACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQL
                     VQVTRRMAELIVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPADP
                     FCFADIGPLLATALHDHPRLREMNVQFPAQTVRATVIGAGAHTLSLSGSTIWLEGVQL
                     PLRNLPVAIPIDETDLVSAWQQALLQLDLDPKTDAYVLALPASLPVRYAAVLTVINAL
                     VDFVARFPNPHPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPL
                     FGGSVVPVTVKSLAFPS"
     gene            complement(22448..23674)
                     /gene="eutH"
                     /locus_tag="JNC24_000527"
     CDS             complement(22448..23674)
                     /gene="eutH"
                     /locus_tag="JNC24_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HAY9157368.1"
                     /translation="MGINEIIMYIMMFFMLIAAVDRILSQFGGSARFLGKFGKSIEGS
                     GGQFEEGFMAMGALGLAMVGMTALAPVLAHVLGPVIIPVYEMLGANPSMFAGTLLACD
                     MGGFFLAKELAGGDVAAWLYSGLILGSMMGPTIVFSIPVALGIIEPSDRRYLALGVLA
                     GIVTIPIGCIAGGLVAMYSGVQINGQPVEFTFALILMNMIPVIIVAILVALGLKFIPE
                     KMINGFQIFAKFLVALITLGLAAAVVKFLLGWELIPGLDPIFMAPGDKPGEVMRAIEV
                     IGSISCVLLGAYPMVLLLTRWFEKPLMSVGKVLNMNNIAAAGMVATLANNIPMFGMMK
                     QMDTRGKVINCAFAVSAAFALGDHLGFAAANMNAMIFPMIVGKLIGGVTAIGVAMMLV
                     PKEDATTAKTEAEAQS"
     gene            complement(23991..25178)
                     /gene="eutG"
                     /locus_tag="JNC24_000528"
     CDS             complement(23991..25178)
                     /gene="eutG"
                     /locus_tag="JNC24_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization ethanol dehydrogenase
                     EutG"
                     /protein_id="HAY9157369.1"
                     /translation="MQNELQTALFQAFDTLNLQRVKTFSVPPVTLCGPGSVSSCGQQA
                     QTRGLKHLFVMADSFLHQAGMTAGLTRSLAVKGIAMTLWPCPVGEPCITDVCAAVAQL
                     RESGCDGVIAFGGGSVLDAAKAVALLVTNPDSTLAEMSETSVLQPRLPLIAIPTTAGT
                     GSETTNVTVIIDAVSGRKQVLAHASLMPDVAILDAALTEGVPSHVTAMTGIDALTHAI
                     EAYSALNATPFTDSLAIGAIAMIGKSLPKAVGYGHDLAARESMLLASCMAGMAFSSAG
                     LGLCHAMAHQPGAALHIPHGLANAMLLPTVMEFNRMVCRERFSQIGRALRTKKSDDRD
                     AINAVSELIAEVGIGKRLGDVGATSAHYGAWAQAALEDICLRSNPRTASLEQIVGLYA
                     AAQ"
     gene            complement(25168..26004)
                     /gene="eutJ"
                     /locus_tag="JNC24_000529"
     CDS             complement(25168..26004)
                     /gene="eutJ"
                     /locus_tag="JNC24_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutJ"
                     /protein_id="HAY9157370.1"
                     /translation="MAHDEQWLTPRLQTAATLCNQTPAATESPLWLGVDLGTCDVVSM
                     VVDRDGQSVAVCLDWADVVRDGIVWDFFGAVTIVRRHLDTLEQQFGRRFSHAATSFPP
                     GTDPRISINVLESAGLEVSHVLDEPTAVADLLQLDNAGVVDIGGGTTGIAIVKKGKVT
                     YSADEATGGHHISLTLAGNRRISLEEAEQYKRGHGEEIWPTVKPVYEKMVDIVARHIE
                     GQGITDLWLAGGSCMQPGVAELFRKQFPALQVHLPQHSLFMTPLAIASSGREKAEGLY
                     AK"
     gene            complement(26015..27418)
                     /locus_tag="JNC24_000530"
     CDS             complement(26015..27418)
                     /locus_tag="JNC24_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase EutE"
                     /protein_id="HAY9157371.1"
                     /translation="MNQQDIEQVVKAVLLKMQSSDTPPAAVHEMGVFASLDDAVAAAK
                     VAQQGLKSVAMRQLAIAAIREAGEKHARDLAELAVSETGMGRVEDKFAKNVAQARGTP
                     GVECLSPQVLTGDNGLTLIENAPWGVVASVTPSTNPAATVINNAISLIAAGNSVIFAP
                     HPAAKKVSQRAITLLNQAIVAAGGPENLLVTVANPDIETAQRLFKFPGIGLLVVTGGE
                     AVVEAARKHTNKRLIAAGAGNPPVVVDETADLARAAQSIVKGASFDNNIICADEKVLI
                     VVDSVADELMRLMEGQHAVKLTAEQAQQLQPVLLKNIDERGKGTVSRDWVGRDAAKIA
                     AAIGLNVPQETRLLFVETTAEHPFAVTELMMPVLPVVRVANVADAIALAVKLEGGCHH
                     TAAMHSRNIENMNQMANAIDTSIFVKNGPRIAGLGLGGEGWTTMTITTPTGEGVTSVR
                     TFVRLRRCVLVDAFRIV"
     gene            complement(27430..27717)
                     /gene="eutN"
                     /locus_tag="JNC24_000531"
     CDS             complement(27430..27717)
                     /gene="eutN"
                     /locus_tag="JNC24_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutN"
                     /protein_id="HAY9157372.1"
                     /translation="MKLAVVTGQIVCTVRHHGLAHDKLLMVEMIDPQGNPDGQCAVAI
                     DNIGAGTGEWVLLVSGSSARQAHKSETSPVDLCVIGIVDEVVSGGQVIFHK"
     gene            complement(27824..28117)
                     /gene="eutM"
                     /locus_tag="JNC24_000532"
     CDS             complement(27824..28117)
                     /gene="eutM"
                     /locus_tag="JNC24_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HAY9157373.1"
                     /translation="MEALGMIETRGLVALIEASDAMVKAARVKLVGVKQIGGGLCTAM
                     VRGDVAACKAATDAGAAAAQRIGELVSVHVIPRPHGDLEEVFPIGLKGDSSNL"
     gene            complement(28156..29172)
                     /gene="pta"
                     /locus_tag="JNC24_000533"
     CDS             complement(28156..29172)
                     /gene="pta"
                     /locus_tag="JNC24_000533"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HAY9157374.1"
                     /translation="MIIERCRELALRAPARVVFPDALDQRVLKAAQYLHQQGLATPIL
                     VANPFELRQFALSHGVAMDGLQVIDPHGNLAMREEFAHRWLARAGEKTPPDALEKLTD
                     PLMFAAAMVSAGKADVCIAGNLSSTANVLRAGLRIIGLQPGCKTLSSIFLMLPQYSGP
                     ALGFADCSVVPQPTAAQLADIALASAETWRALTGEEPRVAMLSFSSNGSARHPCVANV
                     QQATEIVRERAPKLVVDGELQFDAAFVPEVAAQKAPASPLQGKANVMVFPSLEAGNIG
                     YKIAQRLGGYRAVGPLIQGLAAPMHDLSRGCSVQEIIELALVAAVPRQTEVNRESSLQ
                     TLVE"
     gene            complement(29169..29972)
                     /gene="eutT"
                     /locus_tag="JNC24_000534"
     CDS             complement(29169..29972)
                     /gene="eutT"
                     /locus_tag="JNC24_000534"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization cob(I)yrinic acid
                     a,c-diamide adenosyltransferase EutT"
                     /protein_id="HAY9157375.1"
                     /translation="MKDFITEAWLRANHTLSEGAEIHLPADSRLTPSARELLESRHLR
                     IKFIDEQGRLFVDDEQQQPQPVHGLTSSDEHPQACCELCRQPVAKKPDTLTHLSAEKM
                     VAKSDPRLGFRAVLDSTIALAVWLQIELAEPWQPWLADIRSRLGNIMRADALGEPLGC
                     QAIVGLSDEDLHRLSHQPLRYLDHDHLVPEASHGRDAALLNLLRTKVRETETVAAQVF
                     ITRSFEVLRPDILQALNRLSSTVYVMMILSVTKPPLTVKQIQQRLGETQ"
     gene            complement(29969..30670)
                     /gene="eutQ"
                     /locus_tag="JNC24_000535"
     CDS             complement(29969..30670)
                     /gene="eutQ"
                     /locus_tag="JNC24_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutQ"
                     /protein_id="HAY9157376.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEARKVADLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLEQGAMQPSFKSVTGKGGIKVIDGSSVKFGRFDGAQPHCVGLTDLVTGDDGSSMAAG
                     FMQWENAFFPWTLNYDEIDMVLEGELHVRHEGETMIAKAGDVMFIPKGSSIEFGTTSS
                     VKFLYVAWPANWQSL"
     gene            complement(30645..31124)
                     /gene="eutP"
                     /locus_tag="JNC24_000536"
     CDS             complement(30645..31124)
                     /gene="eutP"
                     /locus_tag="JNC24_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HAY9157377.1"
                     /translation="MKRIAFVGSVGAGKTTLFNALQGNYTLARKTQAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            complement(31137..31472)
                     /gene="eutS"
                     /locus_tag="JNC24_000537"
     CDS             complement(31137..31472)
                     /gene="eutS"
                     /locus_tag="JNC24_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HAY9157378.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGVIGIM
                     TLTPGETAMIVGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            complement(31765..34044)
                     /gene="maeB"
                     /locus_tag="JNC24_000538"
     CDS             complement(31765..34044)
                     /gene="maeB"
                     /locus_tag="JNC24_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HAY9157379.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGDQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRERAPELMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            34333..35283
                     /gene="tal"
                     /locus_tag="JNC24_000539"
     CDS             34333..35283
                     /gene="tal"
                     /locus_tag="JNC24_000539"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HAY9157380.1"
                     /translation="MNELDGIKQFTTVVADSGDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGRVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     AQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQYAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            35303..37306
                     /gene="tkt"
                     /locus_tag="JNC24_000540"
     CDS             35303..37306
                     /gene="tkt"
                     /locus_tag="JNC24_000540"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HAY9157381.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKDIYHAWDAREKGEKAQQRWNEKFAAYKKAHPQLAEEFTRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPLLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQVEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEITRGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(37402..38445)
                     /locus_tag="JNC24_000541"
     CDS             complement(37402..38445)
                     /locus_tag="JNC24_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HAY9157382.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAALAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQMIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDAWPTLWITR"
     gene            complement(38571..39146)
                     /gene="nudK"
                     /locus_tag="JNC24_000542"
     CDS             complement(38571..39146)
                     /gene="nudK"
                     /locus_tag="JNC24_000542"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HAY9157383.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKTVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQTSHLMD"
     gene            complement(39214..41193)
                     /gene="aegA"
                     /locus_tag="JNC24_000543"
     CDS             complement(39214..41193)
                     /gene="aegA"
                     /locus_tag="JNC24_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HAY9157384.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKCGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHLTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDSLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRLVPIEG
                     SEFVMPADAVIMVFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            41399..43099
                     /gene="narQ"
                     /locus_tag="JNC24_000544"
     CDS             41399..43099
                     /gene="narQ"
                     /locus_tag="JNC24_000544"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
                     /protein_id="HAY9157385.1"
                     /translation="MIVKRPVSASLARAFFYIVLLSILSTGIALLTLASSLRDAEAIN
                     IAGSLRMQSYRLGYDLQSGSPQLNAHRQLFQQALHSSVLTNLNVWYVPEAVKTRYAHL
                     NANWLEMNNRLSKGDLPWYQANINNYVNQIDLFVLALQHYAERKMLLVVAISLAGGIG
                     IFTLVFFTLRRIRHQVVAPLNQLVTASQRIEHGQFDSPPLDTSLPNELGLLAKTFNQM
                     SSELHKLYLSLEASVEEKTRDLHEAKRRLEVLYQCSQALNTSQIDVHCFRHILQIVRD
                     NEAAEYLELNVGENWRISEGQPNPELPMQILPVTMQETVYGELHWQNSHVSSSEPLLN
                     SVSSMLGRGLYFNQAQKHFQQLLLMEERATIARELHDSLAQVLSYLRIQLTLLKRSIP
                     EDNATAQSIMADFSQALNDAYRQLRELLTTFRLTLQQADLPSALREMLDTLQNQTSAK
                     LTLDCRLPTLALDAQMQVHLLQIIREAVLNAMKHANASEIAVSCVTAPDGNHTVYIRD
                     NGIGIGEPKEPEGHYGLNIMRERAERLGGTLTFSQPSGGGTLVSISFRSAEGEESQLM
                     "
     gene            43263..46376
                     /gene="acrD"
                     /locus_tag="JNC24_000545"
     CDS             43263..46376
                     /gene="acrD"
                     /locus_tag="JNC24_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HAY9157386.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGIGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASGANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENIERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMILTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNKIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVA
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(46475..46534)
                     /gene="ypfM"
                     /locus_tag="JNC24_000546"
     CDS             complement(46475..46534)
                     /gene="ypfM"
                     /locus_tag="JNC24_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HAY9157387.1"
                     /translation="MIERELGNWKDFIEVMLRK"
BASE COUNT        10704 a        13153 c        12107 g        10783 t
ORIGIN      
        1 ccatcggcgc cattttttat gcttccgcca gcggcggcaa aatcaattca tcgctctcat
       61 gctgctgggt atagcgcatc acttccagta cgcgcaaccc cgcacggtgc accgcatcgt
      121 ttaacgcctt ccctttcagc aaaccactga taagctgagc acaaaacagg tcgccagtcc
      181 ctttcaggtc ggtttttacc cgtgaatggg aaatgacatt cacgctgtcg gcactgacca
      241 ccaccacctg catctcctga ttttcttcat taccggaggc gctggtaatc accacccatt
      301 ttaatgtgtc tgaaagcaga ctttttgcgg cagcaatggc actgtcgaga tcgcggcaat
      361 ttttaccggt caggatttcc agctcaaaga tattgggggt aattccttgc gccagcggca
      421 gtaaatattg tcgatacgcc tcgggaaggt caggtttgac ataaattccg ctatcaatat
      481 cgccaatcac cggatcgacc atgatcaata ggtcaggatg gtctttgcgt agcgcagtca
      541 gccactcggc aaggattttg atttgcgatg ccgttcccat atagcccgtg gttacagcac
      601 gaagttggcg cagcgcatca cgctcctgaa gcgcacgcaa atagccgcta aaccattcgt
      661 ccggaatcgc accaccgtag aaagtgtcat aatgcggcgt attgctcagc aataccgtcg
      721 gcacggcaaa gacattcaag ccgttctgtt ttatagcagg caccgcaatg ctgttgccta
      781 cgctgccgta cactacctgc gactgcacgg cgacgatatc cgcctgcagc gccctactct
      841 tatcgttaaa caacaacaaa ctactcattt aattttttct ccttgccgat gatcctcatc
      901 gtattccaac cgaaacttta cctgattctg gcagtcaaat cggctatcac aaaacaagga
      961 taaggtaatt caatgaagaa aatcatttgt ctggtcatta cactactaat gacactcccc
     1021 gcttacgcga agttaactgt tcatgaagaa gcccgcatca acgccatgct ggagggatta
     1081 gcacagaaaa aggatttgat atttgtgcgc aacggtgatg aacatacctg cgatgaagcg
     1141 gtttctcatc tgcgtctgaa gctcggcaat acccgtaacc gcattgacac tgccgagcag
     1201 tttattgata aggttgcttc gtcgtcatcg attaccggga agccgtatat tgtgaagatc
     1261 cccggtaaga gcgatgagaa cgcacagcct tttttacatg ccttaattgc gcagacggat
     1321 aaaacggtgc ctgcgcaata atcttaaatc cccgccccct gactaaaatg ctcttcccca
     1381 aacaccccgg tggaaaggta gcgatcgcca cgatcgcaga tgatcgctac caccaccgca
     1441 ccagggttag cttttgccac ccgcagtgct ccggcaaccg cgccgccgga gctgacgcca
     1501 cagaatattc cttcccgcac tgccagttcg cgcatggtgt tttctgcatc gcgctgatga
     1561 atatccagca cctcatccac cagagaagcg ttgaaaatcc ccggcagata ttccgcaggc
     1621 cagcggcgaa tgccgggaat actgctgcct tcttccggct gcaggccaac aatcgttact
     1681 ggtttggatt gttcgcgcat aaagcgtgaa acgccggtga tagtgccggt tgttcccatg
     1741 ctggagacaa aatgggtaat gcgcccgccg gtttgctgcc agatttccgg cccggtagtg
     1801 gtgtaatggg cgtaagggtt atcgggatta ttgaactgat cgagcagctt tccttcgcca
     1861 cgattcgcca tctccagcgc cagatcgcgc gcaccttcca tgccctgctc tttggtgaca
     1921 agaatcagtt ccgcaccata agcacgcatc gccgcacggc gttcctggct catgttgtcg
     1981 ggcatcagca atttcatgcg atagcctttc agcgcggcaa tcattgccag cgcaatgccg
     2041 gtgttaccac tggtggcttc gattaagaca tcacccggtt taatttcccc gcgcttttcc
     2101 gcctcgacga tcatcgaaag tgccgcacga tctttcaccg aacctgccgg gttattgcct
     2161 tccagtttta accacacttc actgccgtta tccggcccca ttcgctgcaa cttcaccaga
     2221 ggcgtattgc ctattgtttg ttctaatgta ctcacgatct ctatccatac gtggtgttgc
     2281 ctgatgcgac gcttgcgcgt cttatcaggt ctacaggtta caaaccttgc cattaaaaag
     2341 cccggatcgc gggaagcgcc tccgggcgtt taacattcac tcaacctatc aggcgctttg
     2401 tgcgagagca agttcctcat cgcgggtttc gatacgctcg tcgccgttat acagccgcgc
     2461 atgttgcaga ccaacgaata aacgctcgcc acgctgcggg gcatcgtcgc catgcatcac
     2521 gaccgtcagc ggttcgttgt accaccccag cggctgcacc actaattggg tgtagtgacc
     2581 tttcgggctg gcttccagta cctgtaccgg cagcggcgaa tcgagactgg tacggcggct
     2641 gatatccact tcccacgggc gcaggaagag atccaccggc ccctgataag caggtgtgta
     2701 gcccagcggc cagcgatgtg cgccaacatg gaactgcccg ccgcgaatgg ttccctgcag
     2761 gcggttcact tcgcccataa attcgagcac aaaacgggtc gctggttcgc gccatacctg
     2821 ctccggcgca tcagcctgtt caatattgcc ctggctcatc accactacac gatcagctac
     2881 ttccgtcgct tcttcctggt cgtgggtcac gaacacgctg gtgaatttca gctcttcatg
     2941 cagttgacgc agccagcgac gcagctcttt acgcacctgc gcatccagcg cgccaaacgg
     3001 ttcatcaagc agcagaattt gcggttctac agccagcgcg cgcgccagcg ccacgcgctg
     3061 tttctggccg ccggaaagct gtgccggata acgatccgcc agatgagcaa gctgcaccat
     3121 ctccagcaat tttgtcactt tcgctttgat ggctgcggca ttcgagcgat cgcgacgcgg
     3181 cagcaccgtc aggccaaaag cgatattgtc gaataccgtc atatggcgga acagcgcgta
     3241 atgctggaac acgaaaccga ccttgcgatc gcgcgcgtgc aggcggctga cgtcggtgcc
     3301 gtggaagcga atatgcccgc tggtttgatg ctccagcccg gcgataatgc gcagcagcgt
     3361 ggttttcccg gaaccggacg gccccagcaa cgcgaccatc tgacctgaag gaatatccag
     3421 tgagatatcg ttcagcacct gggtgcgacc aaacgacttc ttaatattgg caatctcaat
     3481 gctcatgatg ttcctcctgc tgtgcgcgtt tttcctgatt ctccaggcgc cactgcaaca
     3541 tactttttaa aaacagggtg ataatcgcca tcagcgttaa cagtgccgca gcggtaaagg
     3601 agccgacggt gttgtagtcc tgctccagca attcaatctg taacggcagc gacagggttt
     3661 cgccgcgaat cgagccggaa accaccgaca ccgcgccaaa ctcgccaatt gcgcgggcgt
     3721 tggtcaacac cacgccataa agcagcgccc agcgaatgtt cggcaatgtg acgcggcgaa
     3781 acatctgcca gccagacgcg ccaagcaaaa tcgccgcttc gtcttcctgg ctgccctggc
     3841 ttaacatcac cggcaccagc tcgcgcacca caaacggaca cgtcacaaag atggtgacca
     3901 gcaccattcc gggccaggag aacataattt gcagattatg ttcatccagc cagccgccca
     3961 gcgggccgtt agagccgtag aacagcagat acaccagacc ggcaaccacc ggcgatacgg
     4021 caaacggaat gtccagtagc gtcagcagta actggcgtcc agggaagtta aagcgcgtca
     4081 ccagccaggc cagcagaatg ccgaacacca ggtttaccgg tacggcaatc agcgcgatca
     4141 tcaccgtcag ccagatggcg tgcagcatgt ccggatcggc cagattctgt aaaaccggca
     4201 tcagcccttt gctgaacgcc tgtacgaaga tataaatcat cggcaccagc aggatgaacg
     4261 ccgataccag catcccgatg ccaatcagaa accatttgcc ccagttaatc gggcgcgcgt
     4321 cataacgctt caattgggta acttccgcca ttaatgacct accacacgcc gaccaaagcg
     4381 actttgcaga gtgttaattg agaacagcag cagcagagat gccgcgagga tcaccgaagc
     4441 aatcgcgctc gctgccgggt aatcaaactc ctgtaagcgc acaaaaatca tcagcgacgt
     4501 cacttccgtc ttccacgcga tatttccggc gataaaaatc accgcgccaa attcaccaag
     4561 actacgggta aacgacagcg ccacgcccgc caccagcgcc ggagaaagtt ccggcagcac
     4621 cactttgcag aaactctgcc agcgcgttgc gccaagcgtt tctgccgctt cttcatattc
     4681 cgggcctaac tcttccagca ccggctgcac ggtacgtacc acaaacggaa tgctggtaaa
     4741 ggccatcgcc accgcaatcc ccagccaggt ataggtgact ttgatatcaa acttcgccag
     4801 ccattcaccg taaaaaccgt ttacagaaaa gagcgaggct agcgttaaac cggcgactgc
     4861 cgttggcagc gcaaagggta aatccatcag cgcatcaagc agcgtgcggc ccggaaagcg
     4921 atagcgggtt aagatccacg ccatcagcag gccaaaaacg ccgttaaaaa tcgatgccac
     4981 aaacgctgac agcagcgtta ctttgtaggc cgctactacc tgcgggttgg tgatcacctc
     5041 ccagtactgc gcccagctca tctgagccag ctgcatcacc agcgcggaga gcggcagcag
     5101 caaaatcagg cacacaaaca gcagactggt gccgaggctt aaggtaaagc ccggcagcac
     5161 gcgtcttgat gaaactgcaa acattaatta cgccccgccg ctaacagctt gtctaactcg
     5221 ccgccgctgg tgaagtgggt tttcatcact tccggccagg agccaaattt gtcttccacg
     5281 cggaacagct cggtctgcgg gaatttgtct ttcagtttat ccatgacttc cgggttattc
     5341 acgcggtagt aatagtcggt gatgatggtt tgcgcctgcg ggctgtagag ccagttaagg
     5401 taggctttgg cggctttttc cgtaccgttg gcctgcacgt ttttatcaac ccacgccacc
     5461 gggaattccg ccagaatgtt ggttttcgga atcaccactt caaaaccctg cgcttcatac
     5521 tgtttacgga tgttgttcac ttccgactcg aagctgatca gcacatcgcc caggccgcgc
     5581 tcggcaaaag tggtggtcgc gccacgaccg ccagtatcga acacttcaac gtttttcagg
     5641 aactgggtca taaactgttc ggttttggct ttatcgccac cgtcagcttt atccgctgcg
     5701 ccccatgccg ccagataggt gtaacgcgcg ttacccgacg ttttcgggtt cgggaaaatc
     5761 agcttcacat cggagcgcac caggtcgttc cagtcgtgga tattcttcgg gttgccctta
     5821 cgcaccagga agcccatggt ggagtagaat ggcgagctat tatttggcag gcgcgtttgc
     5881 cagtccgccg ggatcagctt gcctttgtca tgcaggattt gtacgtcagt cacctggtta
     5941 taagtgacaa cgtctgcttt taagccctgc aaaattgcca gcgcctgttt tgatgacccg
     6001 gcatgagatt gttttattgt cagtttgtcg ccgccgtttt ctttggtcca ttgttgctca
     6061 aacggcgggt tcagggcggc aaacagctcg cgggagacgt cataagaact gttcagcagt
     6121 tccgttgcct gtacatggcc cgccagcagc agagaagcga ccagcgcgag tgagttcttt
     6181 ttcagtaagt taacggccat tgcgcaccct tataaattta atgactttct aatagccatc
     6241 atatttataa cggacgtgaa aggagtaacg gttttatata ccgtttggtg atttggaagt
     6301 tgaaaaggga ataagaaccg ggtttaaaac gaaatcctcc ccacaaatgc agggaggaaa
     6361 cagaggtgaa tcagataccg acgctaaccg tctccggcag tgtgctgccg ccatcaatca
     6421 cattctgtgt accggttaaa tagctggatt catccgatgc gaggaaggcc gccagttcgc
     6481 cgacttccag cggatcggcg aggcgacgca gcgggattgc tttcgccatt tcagtcagca
     6541 ccgactctgg atcttccggg ttcgactggc gggcaatgct ttccgccatt ggtgtgcgca
     6601 cgtatcccgg gcaaatggcg ttaacgcgaa taccagactg cgcgtactcc accgccagcg
     6661 attttgtcag gccaacaatc gccgctttcg ttaaggcgta cgccgtttcg ccaggatcgg
     6721 ccaccatatc accagtgact gaagacatca tcacaatgcg accatctttg cgggcaatca
     6781 tctccggcag caccgccttc gtgacgttcc atacgccttt aatattgatg tcaatatgga
     6841 aatcgcgatc gtcatcgctc atatcgagga aactgcccag acgacaaacg cctgcgttat
     6901 tcaccaggat atcaatgcgc ccttcttttt ccttcgcgcg tttgatagct gcggctaccg
     6961 acgccgggtc acgcacatcg gcgacaaccg ccgtacagcg atgaccacga ccacacagtt
     7021 cgtccgccag cttttcgatc tcaggggaga tatccagcaa gattaggttt gcgccatgac
     7081 gcgcaaaagt tctggcaatt ccttcgccaa ttccctgcaa tgcgcccgta atcagtgctg
     7141 tcttgcccgt gagtttaccc attttaatgc tctccttgtt aatacagagc gttacactcc
     7201 attccctctg aaaatacagg ataaacaaaa tattgctgcg gatcagctca ctaattcagg
     7261 aaaatctcaa tccagtcgct gctttatgca tcatttcagg cgttgtgtta gctcactgct
     7321 gcgctgaatc attttcaatg actccacatc attgagttgc acaagaccaa caaacagtaa
     7381 atcggtaaca gaattttgcg ccgtgcgggt agacatcgac gagctacgcc attcggtttc
     7441 accggaaacg gtatcgagcg tgaaatgcgc cagccgccgc agcggagagt cagccagaga
     7501 agtaatggca atcaccgttg ccccctgctt tcgtgccgct tctgcgcaca agacaatctc
     7561 ttttttactg ccgctgtaag agatagcgat ttgtacatcg ccttttttca acgcctggga
     7621 aacggtggct tgtacgtgtg tgtcggcttc ataggccacg cgatagccaa ttttcatcag
     7681 tttgaacgac agatcgcgcc ccaccagcgc cgacccgccc aggcccgtta tctggataaa
     7741 cggtgcttta ctgatgacct caatgatttt ttgcagccga gcgtagtcga acaacgcaca
     7801 ggtttgctcc agcgccagct ctttttcgcg attcagtttg cgggcaatca cttccagcga
     7861 atcatcactg gtaatcgaac tgtgcaggtg cagcgccgtg gcattcgttt tctcccggct
     7921 ggcactgtat tcgccaatta acgccatacg caattcagta aatccttgcg cgccgagctt
     7981 ctgggcaaat ttcacaatgc tcgactgact gatgccgagc tgtttcgcca tcttgcgcga
     8041 agagacggat ttcagctcac tcacgttggc ccgcagaaag tcagcaattt tttgctcgtt
     8101 ctcggtaaat tcactttccg cattgcgtat tttcgtcagg tacaacatct tccactctcc
     8161 cgggctggca gcaaaaattc cgcttaagtt cttatgacgc tcttcacact ctgcaagtgt
     8221 agactcaatg attcctttaa gtctgtcaat tcgggatata tgattccata catctgcaat
     8281 tatttgatcg taaatagtaa ggtcaccacc gatgcaactt gaaaagatga ttactgaagg
     8341 ctcgaacacc gcctcggctg aaattgaccg cgtatcgacg ctggaaatgt gccggattat
     8401 caacgatgaa gataaaaccg taccgcttgc cgttgagcgc gtcctgccgg atatcgccgc
     8461 ggcgatcgat gttatccacg cccaggtaag cggcggcggt cgtctgattt atctcggtgc
     8521 gggaacatcc ggtcgtctgg ggattctgga tgccagcgaa tgtccgccca cctacggcgt
     8581 gaaaccgggt ctggtggttg gtttgattgc tggcggcgaa tatgccattc agcacgcggt
     8641 ggaaggcacg gaagatagcc gagaaggcgg cattaacgat ctgaaaaata taaatttaac
     8701 ggcacaggat gtggtggtcg gtattgccgc cagcggtcgt acgccgtatg tgattgccgg
     8761 actggaatac gcccgccagc tcggttgccg cacagtggga atttcctgta atccggggag
     8821 tgccgtttcc accaccgccg agtttgccat tacaccgatt gtaggtgccg aagtggtcac
     8881 tggttcttcg cgaatgaaag caggcacagc gcagaaactg gtgctcaata tgctttccac
     8941 cgggctgatg attaaatccg gcaaagtgtt cggcaacctg atggtcgatg tggtcgccac
     9001 caacgaaaaa ctgcatgtgc gccaggtcaa tatcgttaaa aacgccaccg gatgtaatgc
     9061 cgaacaagcg gaagcggcgt taattgcctg cgagcgcaac tgtaaaacgg ccattgtgat
     9121 ggtgctgaaa aatctcgatg ccgcagaagc taaaaaacgc ctggatcaac acggcggctt
     9181 tattcgtcag gttttagaca aggaataacc catggccaaa gagatcagca gtgaacttct
     9241 gaacaccatt cttacccgtg tcggcggact gggaaatatc gccagttgtg gtaactgtat
     9301 gacgcgcctg cgtctgggtg tacatgacag ttcactggtt gatcccaata tcaaaacgct
     9361 ggaaggcgtg aagggcgtca ttttgaccag cgaccaggtg caggtcgttt ttgggcctgg
     9421 taaagcgcat cgtgccgcaa aagcgatgag cgagctgctg ggcgaggcac cggtacagga
     9481 tgccgcagaa atcgccgccc agaacaaacg tcagttaaaa gccaaacaaa cctccggcgt
     9541 gcaacaattt ctcgccaaat tcgccaccat cttcacgccg ctgatccccg gttttattgc
     9601 cgccggtctg ctgctgggga tagcgacgtt aattgccacg gtgatgcacg ttccggcaga
     9661 tgctcagggc acgctacccg acgcgctgaa ttttatgaag gtgttcagca aaggtttgtt
     9721 cactttcctg gtgattctgg tgggctataa cgccgcccag gcatttggcg gcacgggcgt
     9781 caatggcgca attattgccg cactcttttt gctcggttat aaccccgctg caaccaccgg
     9841 tgactacgcc ggttttcacg atttctttgg tctgcccatc gatccgcgcg gcaatattat
     9901 cggcgtgctg attgccgcgt gggcctgcgc ccgcattgaa ggcatggtgc gccgctttat
     9961 gtcggacgat ctcgacatgc tgctgacctc gttaatcacc ctgctgatca ccgctacgct
    10021 tgcgtacctg atcattatgc cgctgggtgg ctggttattc gaaggtatgt catggctgtt
    10081 tatgcacctg aacagtaatc cgttcggttg tgcggtttta gccgggctgt tcctgatcgc
    10141 cgtggtattt ggcgtgcatc agggctttat tcctgtttac ctcgcgttaa tggacagcta
    10201 gggattcaac agcttatttc ccatcctttc aatggcaggc gcgggccagg tgggcgcggc
    10261 gctggcactc tactggcggg cgcaaccgca cagtgcgcta cgcagtcagg tacgcggggc
    10321 gattattccc ggcctgctgg gcgttggtga accgctgatt tacggtgtca ccctgccccg
    10381 catgaagccg tttgttaccg cctgtttagg cggcgcggcg ggcggtttgt ttatcggcct
    10441 gatagcctga tggggtctgc cgatgggctt aaacagcgcc tttgggccgt ctggtctggt
    10501 agcgctgccg ctgatgactt ccgcacaagg catccttccg gccatggcgg tttatgctgg
    10561 cgggattctg gtggcatggg tttgcgggtt tattttcacc acgctctttg gctgccgtaa
    10621 cgtcaatctg gactgatatc atgaaacgga caatgctcta tctttctctg ctggctgtta
    10681 gttgtagcgt cagcgccgca aaataccctg ttctgacaga aagctcgcca gagaaagcag
    10741 ggtttaacgt cgaacggctt aaccagatgg atcgctggat tagccagcaa attgatgtcg
    10801 gttatcccgg cgtaaacctg ctgatcatta aagataatca gattgtgtat cgcaaagcct
    10861 ggggagcggc gaaaaagtac gatggcagcg tgttgatgga acagcctgtc aaagccacca
    10921 ccgggacgct gtatgacctg gcctcaaaca ccaaaatgta cgccacgaac ttcgccctgc
    10981 aaaagctgat gagcgaaggc aaactgcatc ctgacgatcg gattgcgaaa tatattccgg
    11041 ggtttgccga tagtccaaat gacaccatta aaggcaaaaa caccctgcgg atttctgacc
    11101 tgctgcatca cagcggcggt ttcccggcag atccgcaata cccgaataaa gcggtcgcgg
    11161 gcgcgttata ttcccaggat aaaggccaga cgctggagat gatcaagcgc acgccgctgg
    11221 aatatcagcc cggcagcaaa catatctaca gcgatgtcga ttatatgctg cttggattta
    11281 tcgtcgagtc ggttaccggt cagccgcttg accgctatgt tgaagagtcg atttatcgcc
    11341 cgctcggcct gacacatacg gtatttaacc cgctgctgaa aggctttaaa ccgcaacaaa
    11401 ttgccgccac ggaattgaac ggcaataccc gcgatggcgt gatccatttc ccgaatatcc
    11461 gcacctccac tctctggggt caggtgcacg atgaaaaagc cttttattcg atgggcggcg
    11521 tttccgggca cgcaggtttg ttttccaata ccggcgatat tgcggtgtta atgcaaacga
    11581 tgctgaacgg cgggggctat ggcgatgtgc agctgttcag tgcggaaacg gtgaagatgt
    11641 tcaccaccag ctctaaggaa gatgccactt ttggcctcgg ctggcgcgtg aatggtaatg
    11701 ccaccatgac gccgacgttt ggcacgctgg caagcccgca aacctacggt cacactggct
    11761 ggaccgggac agtaaccgtt atcgatccgg tcaatcatat ggcgattgtg atgttaagca
    11821 acaagcccca ttcgccagtt gccgatccac aaaaaaatcc caatatgttc gaaagcggtc
    11881 agttaccgat tgcaacttat ggttgggtag ttgatcaggt gtatgcggcg ttgaagcaaa
    11941 agtaataaaa aggccagtca gttttgttac tggctggcct ttaatcatcg aatcagaaac
    12001 gcttacaacg ccattaactt gtccagcgac ggcgcgaagt aatagccgcc ggttaccggt
    12061 ttggtgaaac gcaacatcgc atcacgctta ccatcggtat cgccaaacat gctcagcagt
    12121 tgctgctcaa tgttatgcag acgcgcgcag taggcgcaga agtacagacc gtgagtgcca
    12181 ctggcggtgc cgtacggcag gctctggcga acaatcttca gccctttgcc atcttctttc
    12241 agatcaacgc gggtgaggtg agaggtttcc ggacgttcgt cgccgtcaat ctcttcgttg
    12301 gcctctttgg tgcgcccgat catcatctcc tgatcgtgaa cgctcatccg gttgagctgc
    12361 ttcaggttgt gctcccaacg ctgcacaaac acatagctgc cgccggcatc cacgccgtct
    12421 ttgataaccg ccacttcgcg acgcgtctct tcacccgccg ggttttctgt accgtcaaca
    12481 aagccgctca ggtcacgctc ttccacccaa cggaagccgt ggatctcttc tttcacttca
    12541 atgcagtcac caaaggcttc catcgccgcc tgggcgacag agaagtttac gtcgtgacgc
    12601 agagaaagaa tgtggatcaa cacatcgaac tgggtggtcg gcgcaaggcc tttaccgtag
    12661 cccggaaaat ctttcagctc ttctgcccca acgccgccgc tcagagcgcg ccaggtgttg
    12721 ttaccaaagg caaccaccgc accaagatgc gcgtccggga atttcgcttc aaaagttgcc
    12781 agtttgtcgg caaatgtttt actggccgca cgcagggcgt caacttcccc tttcacgttg
    12841 gcttcgatcc aaatcgccgc gcggcaatgt tctggcaaaa tgccactctg aacctgagac
    12901 attgttcctc ctgaaaataa gaatgccacg caatccgtgg cattgttgag cgctatttta
    12961 cgtgtttttt cgcggcggcg gtttgttcag acgcaaatta acgccgccag ataattttac
    13021 tgactttcca gtttttcagg gtatcgtccg aaggcattaa cccttcagga ccacgccatt
    13081 ccccgctgaa ctggtagcta atatgttgac tgccttcggc tttacattcg acggcacgat
    13141 tatcatcacc atcagctttt tggcaattgc caaatgcttt gctgtaaagg tcgctaaacg
    13201 gtgtaccgat tttaacaccg gtgtcagcag gaatatcgct atccagcacg tcaatgcgac
    13261 tgatcgtgcc ttgatcgccg ttaatcacca tcgccacgtt gtcgcctttc atcacttcaa
    13321 aaaagcgcac cacgttgccg ttcgcggttt tcattccgct gcgcaggcga taatcgccat
    13381 caagcgcatc agcaatggct tgttcttgca gtggtgtgga cgccgttaat tcacccacgc
    13441 cctgctcgct cactttggtg gacgatccaa accagttcca cgggttagcg gccgaccagt
    13501 taactgacga catcgtggaa cagccggtca gcatcaacgg catagcgcat aacattaaac
    13561 gcagcgattt catgtcactt cctttggtta ttcaataacg ttgcttggag tgcaaattca
    13621 ccaaaaagtg ccgttattct ttttcttcat taaaacaagc gcgtaaacgt cgattggtca
    13681 gcagccagat taacgccaca atatccgcca ctaccagcgc cagaccgata ccgctaacgg
    13741 attcaccgtt cagccacagc cacggttgcc agcaaagtaa aaccacctgc gccaacagca
    13801 acagaaaata gagcacatgc caagtgcgag gaaatgtagc ccgccgaccg ctcaacaaaa
    13861 acgccagcac cgccggaatg cctggaatca gccccagcca gaaattatcg tgatcgggat
    13921 aaaacagatt tagcagcgca gtaccctgct cgcgcgacgc accggcaatg acaaacagca
    13981 cccaggttcg cgcctgaagc aatagcacaa gccagaagag caagggtaaa cgcaggcgtc
    14041 cgtgcgcatc ataatggaca ggatggaact cagtactctt catcttcaat caaacgctta
    14101 cccagactca gcacgtcggc gtgttcatat cccaggcgtt catacattcc gagcaccatg
    14161 tcgttatctt ccggcacatt gatctgaatt ttcgggcagc cacgagcaat cagctttttc
    14221 tccagccgat taagcaacgc attggcaatc ccacgcccac gaaactctgg atgcacgcca
    14281 agataataag cagacccgcg atgcccgtca taaccgccca tcaccgttcc gaccacttca
    14341 ccgtttacct cagcgaccaa aaacaaactg acgtcatggt tcatcttacg ctcgatgtcc
    14401 atttccggat cgttccacgg acgcagcaag tcgcaacgct cccaaagggt gatgacctct
    14461 tcgaaatctt cctggcgaaa tacgcgtatc tccatggtat tcgttacctt tttgcgggtt
    14521 aaaaggctga ttatggcgtg aacggtcgaa ttagccaata tctgacgaaa atcggttgaa
    14581 aaagtggcat aatggggagt tgtcaactat tgaaatgaaa agtaaaacaa ttctcaacat
    14641 caaaccgtcg taacggatta cgcgatacga tataacatct ggaactttat tattacaact
    14701 caggccgtat gagcactttt aaaccactaa aaacactcac ttcgcgccgc caggtgctga
    14761 aagccggttt ggctgccctg acgttgtcag gaatgtcgca agccatcgcc aaagacgaac
    14821 ctttaaaaac cagcaacgga cacagcaagc cgaaagccaa aaaatctggc ggcaaacgtg
    14881 tcgttgttct cgatccaggc cacggcggaa ttgataccgg agcgatcgga cgcaacggtt
    14941 cgaaagaaaa acatgtggtg ctggcgattg ctaaaaacgt ccgttccatt ttgcgtaatc
    15001 atgggattga tgcgcgttta acgcgttctg gcgatacgtt tatcccactt tacgatcgcg
    15061 ttgaaatcgc ccataaacat ggcgcagatc tgtttatgtc aattcatgcc gatggcttta
    15121 ccaacccgaa agctgccggt gcttcggtat ttgccctctc taaccgtggg gcaagtagcg
    15181 caatggcgaa atacctgtct gaacgcgaaa accgcgccga tgaagttgcc ggtaaaaagg
    15241 cgactgacaa ggatcaccta ttgcagcaag tgctgtttga tctggtgcaa accgatacta
    15301 ttaaaaacag cctgacgctt ggctcgcata ttctgaaaaa gattaagccg gtgcataaac
    15361 tgcacagccg caacaccgaa caagccgcgt ttgtggtgtt gaaatcgccg tcggttcctt
    15421 cggtgctggt ggaaacctcg tttatcacca acccggaaga agagcggctg ttaggcacgg
    15481 cggcgtttcg tcagaaaatc gccacagcga ttgctgaagg cgtgatcagt tatttccact
    15541 ggttcgacaa ccagaaagca cattcgaaaa agcgataagt tatgaaaccc gacgcacacc
    15601 aggttaaaca gtttctgctc aaccttcagg atacgatttg tcagcagctg accgccgtcg
    15661 atggcgcaga atttgtcgaa gatagttggc agcgcgaagc tggcggcggc gggcgtagtc
    15721 gggtgttgcg taatggtggt gttttcgaac aggcaggcgt caacttttct cacgtccacg
    15781 gcgaggcgat gcctgcttcc gccaccgctc atcgcccgga acttgccggg cgcagtttcg
    15841 aggcgatggg cgtttcactg gtagtgcatc cgcataaccc gtatgttccc accagccacg
    15901 cgaatgtgcg tttttttatt gccgaaaaac cgggtgcaga gcccgtctgg tggtttggcg
    15961 gcggcttcga tttaacccct ttctatggtt ttgaagaaga cgccattcac tggcaccgca
    16021 ccgcccgtga cctgtgcctg ccatttggtg aagacgttta tccccgttac aaaaagtggt
    16081 gcgacgatta cttctacctc aaacatcgca acgaacagcg cggtattggc gggctgttct
    16141 ttgatgatct gaacacgcca gatttcgacc actgttttgc ctttatgcag gcggtaggca
    16201 aaggctacac cgacgcttat ttaccaattg tagagcgacg taaagcgatg gcctacggcg
    16261 agcgcgagcg caattttcag ctctaccgtc gcggtcgtta tgtcgagttc aatctggtct
    16321 gggatcgcgg cactctgttt ggcctgcaaa ctggcgggcg caccgagtcg atcctgatgt
    16381 caatgccgcc actggtacgc tgggagtatg attatcagcc aaaagatggc agcccagaag
    16441 cggcgttaag tgagtttatt aaggtcaggg attgggtgta actccctcac ccccattccc
    16501 gcatccgctg atgcaacgtc aacgacggct tctcggcaaa cagctgctgg taatccgtgg
    16561 caaattgccc cagatgccag aatccccact gcatggcggc gtcttttacc gttgtgcttt
    16621 gcgaccacgg gcttatcagt tcgcggcgta cggcgttcag gcgaatgcgt ttcagccacg
    16681 cgtttgggcc aatgcctaaa atagcgtgaa acgcgttttg tagcgtgcgg cgactgacat
    16741 gcagttgatt acacaagtcc agcaccgtca ccagctcaga catattttcc agcacatatt
    16801 cacgggcgcg ggaaagtaat ctacggtaac tctgatgact gatgctttcc gccgtcacca
    16861 ttggttgcgc gtcttccagc atcgccccca tcgccattag caaattatcc cccagcactt
    16921 ttcgcactgc aggctgatgg agattttccg ggttctcgca aaacgtcgcc agcgcctgtt
    16981 ggacaaagcc ccacagcgcg gctttatgct gctctttcac ttccagcgcc gactggctac
    17041 gcagcatatg taatacccga tccggattgt gcaaaaagtt agcctgacgg gtgatgacat
    17101 cttcagacag cactacgccc aggatcgtat aatcgtccgg cgtacttaat tcaaactcgg
    17161 ttccaccggg gcgcgtcgct atttctgcgc ttcccagaca ttgcgaaccg ataaatccct
    17221 gctcaccgcg cgtcgccgga atgccaaacc agaacgagtt cggccagacc aggcacgact
    17281 gacgcagcgc cagaccggtg tattcacgaa aaacctgaat atcatcgagc agaatttccg
    17341 taaattcacc atgaaacttg cccggatgca gctgatcgta aatctgctgc caggcggtaa
    17401 tcgtcaaagc atgttcatag acatccgttg tctgtcgttg atgaacatta tccacttcga
    17461 ccttcggcgt gagcttcagg ttttcgggta agggttcatg ataaagatgg tgcaaattgg
    17521 ctgtacgggt ctttttcatg atgttaatgc cgggtgttgt aggacacccg gcacctccga
    17581 caggttaatg gggcttgaga cgataacgac tactgcgttt acgtaacgtc ccggcagaaa
    17641 agagctgttc gagcgcattt ctggcttttt ccagcggcca gccaaagtgg gcagccactt
    17701 ctcctgccgt cattccctga cgtactgatg ttaacagcgc caacaactcg tcagcagatt
    17761 ccgcgacgat aactggcgcg tcgggttcct ttacgggttg cttcggctgg cggttaaagc
    17821 cagtaaccag ccactgggtg tcatcgtctg gccgaccgat ctccttgcgg ctgatgacac
    17881 gcccggtacg catcgcggca gcacaacctg cgtccagcgc cgcacgacac gccgccagat
    17941 cgccttccac caccagcgtt aagcgaccag ggtcaagcac ttcgtgactg agcagacgaa
    18001 cgttagctgc tttgagcatg gcatccgctg catctatcgc ggcgaccatg ccgtccactt
    18061 ccagcaatcc cagtgcattg atcatcggca acctccgtta cgcacgctgg attggattac
    18121 gcgcgatttc cagcactgca tcggtaaagg cgttacaggc tgctttacac gcggcctggc
    18181 taccggttaa aaatgctgcc gagtagttgg tttcagacgg tggcgggaca taggtcacca
    18241 gctgtacgtc ggcagatttc aacgctgcat caatgccgta ggtcgcttcc agcggcggtg
    18301 ccaccagata cgccatcgga tcgccaagcg tgatcccggc ggttgatgaa agataagaac
    18361 cggtacgcga aactacatgt gccaggaatg cggtgttttc tgcgtcatta gcccactgga
    18421 aagcagcgcc attttcaata tgcgcaacca tcgcatccag accagcacgc acttccgccg
    18481 ggtttggccc accaagcata atcagcactt caccggcagt cggtgacggt ccgtgtgctg
    18541 ctcccgcata cagcgagcgg ccatatacca cttcgaccat cgcctgcttg gtcgcttcgt
    18601 cagccgcaat gtaggtgacg tcatcggaat cagcagaaat cagcccgagg ctacgaatat
    18661 gcggcggcaa ttttagttca cgcgcaaatt cagcgttaac ggaggcaatc acccgcatag
    18721 cggttaccga cggtcgaatc aaatctaaag ctggcatgat gcctccttat cgggtcatgt
    18781 tgatgccgga tgctttctgc tccagcatac gtttcgccaa atccacaatg acggctgcgg
    18841 cttcaaccgg cggcgtgccg ccctggtgaa tgttagagat gcaggtgcga tcggcctcaa
    18901 cggtggtcgc catacgcggc gagtaaacgg cgtaacagga gaggctttct gactgaccca
    18961 accccggacg ttcccccacc agcaggatca ccactttcgc gccgaggatc tcaccgatct
    19021 gatcttcaat cttcacgcga ccataacgaa caaagaatgg cgtgccgact ttcagcccgg
    19081 cctgtttcag gcccgccatc agcggcggca ggatctcttc gtagttcacg gtgatggcat
    19141 cagttgacag gccatcagaa ataacgacct gtacgtccgg attagcaaca cactgtgctt
    19201 tcagcgcttc aacagcttct gcacacaggc ggcggcccat atccgggcga gtcaggtaga
    19261 gatttttgtc gctaatttcg gagcgtactt ccagcaagcc ctgcgctttc acccactctt
    19321 ccggcacttc tttcagtacg gtatctttcg aacgagagtg atcggccagg aaacgcagca
    19381 gcgcctgcgt acgtggacgc ggaccggtac gaccggtaca aacgcgggcc acggtgctgc
    19441 ggcgcagttc tgttaatacg tctgcgcgat gcgggttttc aacaccaatc cacgcttttg
    19501 cttccgcaga acctaaatcc agcgcgcagc tttccgaggt caccggtgcc gcacagttag
    19561 tggtggcgca ctttgcttct gacggcgcgg gggccgcttg tcccattgac gccatcacgc
    19621 tgcgtacaat ttcttcaatc tgtttttgat ccatgatgtg ttatctccgc gtcatcagaa
    19681 gaacagtgac ggatcgcccg cccgtttggt caggcgaccg tttgccataa tgcccatgct
    19741 ttccagccag cgttcaaact ccggtgacgg gcgcaggttg agtaactgac gcacagtggc
    19801 agtgtcgtgg aatgcggtgg tctgatagtt gagcatgatg tcatcgccca gcggcatccc
    19861 catgatgtag ttgcagcctg cggtggcgag caggatcatc aggttttcgt tgaggttctg
    19921 gtcagcatca gcgtggttgg tgtaacagca gtcacagccc atagagatgc cgctcagctt
    19981 gcccataaag tgatcttcta agccagcacg gataatctgg cggtcgttgt agagatactc
    20041 cggcccaata aagccgacca cggtgttgac gataaacgga tcgtaatgac gcgccagccc
    20101 atagttacgt gcttccatcg tcacctagtc tgcgccgaag ttagcgccag cagacagcgc
    20161 cgaaccttgc ccggtttcga agtagaggca gttttccccg gcgatacggt tgaactcagc
    20221 acccactgcg cgcgcttcgt cgagcatcgc cagttccacg ccaaactctt tcagcccttt
    20281 ttcgctgcca cagatactct ggaaaatcag tccgccaggc gcgccgcgac gaatcgcttc
    20341 gatctgggtg gtgacgtgcg ccagtacgca gccctgagtt gggatgttga atttgtcgat
    20401 cacgccgtaa atggtgtcca acacgcggct taagttttcc acgtcgtcag ttaccgggtt
    20461 aacgccgatc accgcatcgc ccaccccgaa ggaaagccct tcgtagattt gtgcagcgat
    20521 actttgcacg tcgtcacggg tgtcatttgg ctgcaaacgg gcgctgaagg tgcccggaat
    20581 accgatggtg gtattggcct ttttgattac cggcattttc ttcgcgccgt agatcaggtc
    20641 cgcgttggag caaatcttcg ctaccgccgc gaccacttcc gaggtcagcc ctttgcgggt
    20701 aaaggcaatg tcgtccacgc tggtttcatc gctcagcaca tactcacgca gttcgctgat
    20761 gctcctgttt ttaatctggt tgtaggccgt ttcgttaaca tcgtcctgaa tcagccgcgt
    20821 cacgcagtca tcttcatagg caatcaccgg attattgcgg atgtccgcta cggtcatttc
    20881 cgacaacacc tgctttgccg ccacgcgctc ctgtgagctt gccgctgcaa cgcccgccag
    20941 cacatccccc gaacgcagtt cgttggcttt agccagcacc tcttttacat ccttaaactg
    21001 atatacattg ccgaacaatg tggtctttag tttcataagt cgttccctca ggaaggaaat
    21061 gcgagtgatt tcaccgtcac cggcacaacc gatccgccaa aaagaggcgt accaatgtcg
    21121 atatagtccc ccgcgcggac aatcacttcg tcaatgactg ccaacgggag ttgttgtagc
    21181 tgtgggcgca acaacatgcc cagagcttta ccaaagtcct gcccggccac caccagcagg
    21241 ggatgcggat ttggaaaacg cgcgacgaaa tcgaccagcg cgttgatgac cgtcagtacc
    21301 gcggcgtaac gcacaggcag cgaggcggga agcgccagca cgtacgcgtc agttttggga
    21361 tcaagatcca gctgaagcag cgcctgttgc caggcactca ccagatccgt ttcatcaatc
    21421 gggatcgcca ccggcaaatt gcgcagcggc agttgtacgc cctcaagcca gattgtgctg
    21481 ccagagagcg aaagggtatg cgcgcccgcg ccaatcaccg tggcgcgcac ggtttgcgcc
    21541 ggaaactgta cgttcatctc acgcaggcgc ggatggtcat gcagcgccgt tgccagcagc
    21601 gggccaatat cggcaaaaca gaacgggtcg gcgggctggt ggcgataaca ttcgcccacg
    21661 ccgccagaaa gcgtaatgat ttcgggcgta acacctgcgg gcagcaaacc ggtttgcatc
    21721 aatgcctgcg cgagcggcga gagcgttccg tcaatcactt cgacaatcag ttctgccatc
    21781 cgccgggtaa cctgcaccag ctgcgcgccg gtcagcgaac gggcgtcggt gcctgcgccg
    21841 aagcactcat ccacaatcat ctgccccggt ttatgagcgt aaaccacgcg cccctggctg
    21901 tcggtttcca gcaggcgacc accgacgttg aggcaggcag tgccgctgat ttttccggca
    21961 tcgaacaggg cgtagttcgc agtgccaccg ccgatgtcaa tattcagtac ccgacacagc
    22021 cgttgttcag aaagggtttg tgccccggca ccgtgaccgg caatgacgga ttcgaggtgc
    22081 ggcccggcgc tggcaacgac aaaatcgccc agcgactggg agagcaccat caccgccgga
    22141 cgagcattgc gggttttcgc gctttcaccg gtgatgatga tggcaccaga atcaacgctt
    22201 tccggcgcaa tgcccgcagc ctgatattgc tcgaggatta aggttttcag ttccgcttct
    22261 tttaaaccac cctgtttatc gacaggggta aagaacaccg ggctttgcca gctaatttcg
    22321 cgtttaatga attcgtagcg cggcacctgc gacaccgccg cacggttaac cagctccagg
    22381 tgggagaaaa tcacctgggt ggtggtggtg ccgatatcga taccgacgct caatagctgg
    22441 cgagtgttca cgattgtgcc tccgcttcgg ttttagccgt ggtcgcgtct tcttttggca
    22501 ccagcatcat cgccacgcca atcgccgtta cgccgccgat caacttgccg acaatcatcg
    22561 ggaagatcat ggcgttcatg ttggcagcgg cgaagcctaa gtggtcgccc agggcgaaag
    22621 cagcggaaac ggcgaaggcg cagttgatga ctttgccgcg ggtatccatc tgcttcatca
    22681 tgccgaacat cgggatgttg ttggcaagcg ttgccaccat gccggctgcc gcgatgttgt
    22741 tcatattcag cactttaccg acgctcatca gcggtttttc aaaccagcgg gtcagcagca
    22801 gcaccattgg atacgcccct aacagaacgc aggagataga accgataact tcaatggcgc
    22861 gcatcacttc accgggttta tcgccagggg ccataaagat agggtccaga ccggggatca
    22921 gttcccagcc aagcagaaac ttaactaccg cggcagcaag accgagggtg atcaatgcaa
    22981 cgaggaattt ggcgaagatc tggaagccgt tgatcatttt ttccgggatg aatttcagcc
    23041 ccagcgccac cagaatcgca acaatgatca ccgggatcat gttcatcagg atcagggcga
    23101 aagtgaattc caccggctga ccgttgatct gcacaccgga gtacatagca accagaccac
    23161 cagcaataca gccaatcgga atggtcacaa tgcccgccag cacgccgagc gccagataac
    23221 gacggtcaga aggttcgata atgccgagcg ccaccggaat ggaaaacaca atcgttggcc
    23281 ccatcatcga cccaagaatt aacccagagt atagccacgc tgctacgtcg ccgcccgcca
    23341 gctctttggc gaggaagaag ccgcccatat cgcacgccag cagcgttccg gcgaacattg
    23401 acgggtttgc gccgagcatt tcgtaaaccg ggataattac cggcccgagt acgtgagcca
    23461 gtaccggtgc cagcgcggtc ataccgacca tcgccagacc cagagcgccc attgccataa
    23521 agccttcttc gaactgaccg cctgatcctt cgatactttt accgaactta ccgaggaaac
    23581 gagcagaacc gccgaactgc gacaggatcc tgtctacggc agctatcagc ataaagaaca
    23641 tcatgatgta catgatgatt tcgttaattc ccatcgcctt tactccctgt tagttgttat
    23701 ttattggcgg atgcggcgta aacgccttat ccgccctaca tgtgcaatcc cgaaggccgg
    23761 atcagacgcg gcaagcgtcg catccggcaa ttgcaccgcg ccactggcgg atgcggcgtg
    23821 aacgccttat ccgccctaca tgtgcaatcc cgtaggccgg ataagacgcg gcaagcatcg
    23881 catccggcaa ttgcaccgcg ccactggcgg atgcggcgtg aacgccttat ccgccctaca
    23941 tgtgcaatcc cgtaggctgg ataagacgcg gcaagcgtcg catccggcat ttattgcgcc
    24001 gccgcgtaca gtccgacaat ctgttccagg ctggcggtac gcgggttact gcgcagacaa
    24061 atatcttcca gcgcggcctg cgcccatgcg ccgtaatgcg cagatgtcgc accaacatcg
    24121 cccagtcgtt taccaatccc aacttccgca atcagctcac ttaccgcgtt aatagcgtca
    24181 cgatcgtcgg attttttagt tcgcagtgcc cgaccaatct gactaaagcg ttcacgacaa
    24241 accatccggt taaattccat caccgttggc agcaacatgg cgttcgccag accgtgtgga
    24301 atatgcagcg ccgcgcccgg ctgatgcgcc atcgcgtggc acaacccaag acccgcactg
    24361 gaaaacgcca ttcccgccat acatgaagcc agcaacatgc tctcgcgcgc ggcaaggtcg
    24421 tgaccgtagc ccaccgcttt cggcagcgat ttgccaatca tcgcaatggc accaatcgcc
    24481 agactgtcgg taaacggtgt agcgttcagg gcgctgtatg cttcaatggc atgggttaac
    24541 gcatcaatgc cggtcatcgc cgtgacatgc gacggcacac cttcggtcaa cgcggcgtcg
    24601 agaatcgcca catccggcat cagcgaggca tgggctaaca cctgcttgcg cccgctcacc
    24661 gcgtcgataa tcaccgttac attggtggtt tcagagccgg ttccggcggt cgttggaatg
    24721 gcaatcagcg gcaagcgcgg ttgcagaacg ctggtttctg acatctctgc cagcgtgcta
    24781 tccgggttcg tcaccagcaa cgccacggct ttcgccgcat ccagcaccga gccgccgcca
    24841 aatgcgatca ccccatcaca gcctgactca cgcaactgcg ccacggctgc acacacgtcg
    24901 gtgatgcacg gttcgcccac cggacatggc cagagcgtca tggcgatgcc tttaacagcc
    24961 aggctgcgcg tcagcccggc ggtcatccct gcctgatgca aaaagctgtc tgccatcacg
    25021 aacagatgtt tcagcccacg cgtttgcgct tgctgtccgc aactgctcac cgagcccgga
    25081 ccgcaaagcg tcaccggtgg aacgctaaat gtttttaccc gttgcagatt cagggtatcg
    25141 aacgcctgaa agagcgcggt ctgcaattca ttttgcatat agcccctccg ctttctctcg
    25201 cccactactg gcgatcgcca gcggggtcat aaacaaactg tgctgcggta aatgcacctg
    25261 taacgccggg aattgtttgc gaaacagttc cgccacgccc ggttgcatac aagagccgcc
    25321 cgccagccat aaatcagtaa tgccctgccc ttcaatatgc cgggcgacaa tgtccaccat
    25381 tttttcgtac accggtttca ccgtaggcca aatctcttca ccgtgaccgc gcttgtactg
    25441 ctccgcctct tccagcgaaa tacggcgatt tccggcgaga gtcagagaga tgtgatgtcc
    25501 gccggttgct tcatccgccg agtacgtcac cttgcccttt ttcacgatgg caatgccggt
    25561 agtgccgccg ccgatatcca ctacaccggc gttgtccagt tgcagcagat ccgcaactgc
    25621 ggttggctca tccagcacat ggctcacttc cagtccagca gactccagca cgttgatgga
    25681 aatacgcggg tccgtacctg gaggaaatga agtcgccgca tggctaaaac ggcgaccgaa
    25741 ttgctgctcg agcgtgtcga gatggcgacg aacaatggtg acagcgccga agaaatccca
    25801 gacgatgccg tcgcggacca catcggccca gtcgaggcat accgccaccg actgaccgtc
    25861 gcggtcgaca accatcgaca ccacatcgca ggtgcccaga tccaccccca gccacagcgg
    25921 agattccgtc gcggcgggcg tctggttaca cagcgttgcc gccgtttgca gacgtggggt
    25981 gagccattgt tcgtcgtgcg ccatctgtta ctccttaaac aatgcgaaac gcatcgacta
    26041 atacacagcg acgcagacgg acaaacgtac gcacgctagt taccccttca ccggttggcg
    26101 tggtgatggt catggtggtc cagccttccc cgcccagccc cagcccggca atgcgcggtc
    26161 cgttcttaac gaaaatgctg gtatcaatag cattcgccat ctggttcatg ttttcgatgt
    26221 tgcgcgagtg cattgccgcc gtgtggtggc aaccgccttc cagtttcacc gccagcgcaa
    26281 tggcatccgc cacgttggcg acgcgcacga cgggcagcac cggcatcatc agttcggtca
    26341 cggcaaacgg atgttctgca gtggtttcca caaacagcaa acgcgtttct tgcggcacgt
    26401 tcaggccgat tgccgccgcg attttcgccg catcgcgacc aacccagtca cggctgacgg
    26461 tgccttttcc gcgctcgtcg atatttttca gcaacaccgg ttgcagctgc tgcgcctgtt
    26521 ctgcggtcag tttcaccgcg tgctggcctt ccatcagacg catcagttca tcggctacgc
    26581 tatcaacaac aatcagtacc ttttcgtcgg cacaaatgat gttgttatcg aaagaagcgc
    26641 ctttgacgat ggactgagcg gcacgggcga ggtcggcggt ttcatccacc actaccggcg
    26701 ggttgccagc gcctgcggca atcagacgtt tattggtgtg tttacgcgcc gcttctacta
    26761 ccgcttcgcc gccggtcacc accagaaggc cgatgcccgg aaacttgaac aaacgctgcg
    26821 cggtttcgat atccggattt gccacagtaa ccagtaagtt ttccggcccg cctgcggcaa
    26881 caatcgcctg gttgagcagc gtgatcgccc gctgggagac ttttttcgcc gccggatgcg
    26941 gggcaaaaat gacgctgttg cccgcggcaa tcaggctgat ggcgttatta attacggttg
    27001 ccgccgggtt agtggacgga gttaccgaag ccaccacgcc ccagggcgcg ttttcaatta
    27061 aagtcaggcc gttgtcgccg gtcagcacct gcggagagag acactcaacg cctggcgtgc
    27121 cgcgcgcctg agcgacgttt ttggcaaatt tatcttcaac gcggcccatg ccggtttcac
    27181 tgacggcaag ttccgctaaa tctctggcgt gtttttcgcc tgcctcacga atggcagcaa
    27241 tggctaactg gcgcattgcc acgcttttta acccttgctg ggcgactttg gctgccgcaa
    27301 cggcgtcatc cagggacgcg aaaacgccca tctcatgaac ggcggcgggc ggcgtgtcac
    27361 tgctttgcat tttcagcagt accgctttca ccacctgttc aatatcctgt tgattcatga
    27421 tgttctgcct tatttgtgga aaattacctg accgccagac accacctcat cgacaatgcc
    27481 aatcacgcac agatcgaccg gtgacgtttc gcttttatgc gcctggcggg cggaactgcc
    27541 actcaccagt aacacccact ccccggttcc cgcgccaata ttgtcgatgg cgacggcgca
    27601 ttgcccgtca ggattacctt gtggatcaat catttccacc atcagcaatt tgtcatgcgc
    27661 cagtccgtga tggcgtacgg tacaaacaat ttgtccagtg acgactgcca gtttcatacc
    27721 cgcctccgtg gcgtatttca ggtaaaagct ccccctaccc tccgcagaag gtaaaatgaa
    27781 aaaggagaga gcgtgacgcc cgaatcgacg tcacacaggg tgattacagg ttgctgctat
    27841 cgcctttcag gccgatcggg aagacttctt ccaggtcgcc gtgcggacgc gggataacgt
    27901 gtacagatac cagctcgccg atacgctgtg ccgctgcggc acctgcatcg gtcgcggctt
    27961 tacaggctgc aacatcacca cgaaccatgg cagtacacag gccgccgcca atctgcttaa
    28021 caccaaccag cttgacgcgc gcagctttaa ccatcgcgtc agaagcctca atcagtgcaa
    28081 ccaggccccg ggtttcgatc attcctaatg cttccattgt gctttcctct ttatcagggt
    28141 ccagaacggg accgttcatt caaccagtgt ttgtaaactg ctttcgcggt tcacttctgt
    28201 ctgacgcggc acagctgcca ccagcgccag ctcgataatt tcctgcacgc tacaaccacg
    28261 agagagatcg tgcatcggcg cggcaagtcc ttgtatcaat ggcccgacgg cacgatatcc
    28321 gccgagtcgt tgtgcgattt tgtagccaat atttccggct tccagcgacg gaaaaaccat
    28381 cacattggct ttgccctgta gcgggctggc aggcgctttt tgcgccgcca cttccggcac
    28441 gaaggcggcg tcaaactgca actcgccatc aaccaccagc tttggtgcgc gctcacggac
    28501 gatttctgtc gcctgctgga cgttggcaac gcaggggtga cgggcgctac cgttactgga
    28561 aaacgacagc atcgccacgc gcggctcttc tccggtgagg gcgcgccagg tttcggcact
    28621 ggcaagcgcg atatccgcca gttgcgccgc cgtcggctgt ggcaccacgc tgcaatcggc
    28681 aaagcccaac gccggaccgc tgtactgtgg cagcatcagg aaaatggatg agagcgtttt
    28741 acagcctggc tgcaagccga taatacgtaa tccggcacgc agcacattcg ccgtggaaga
    28801 gaggttgccc gcgatacaga catccgcttt accggcgctg accattgctg cggcgaacat
    28861 cagcggatcg gtaagttttt ccagcgcatc cggcggcgtt ttttcgcccg cgcgggccag
    28921 ccagcgatga gcaaattctt cccgcattgc gaggttgcca tgcggatcaa tcacctgtag
    28981 cccgtccatc gccacgccgt gactgagcgc aaactgacga agttcaaacg gattagcgac
    29041 cagaatgggc gttgccagac cttgttgatg taaatattgc gcagctttca gcacacgttg
    29101 atctaacgca tccggaaaaa ccactctggc gggcgctcgc aacgccagtt cacgacaacg
    29161 ttcaataatc attgcgtttc ccccagtcgt tgttggatct gcttcaccgt tagcggcggc
    29221 ttcgttacgc tcaggatcat cattacgtag accgtgctcg aaaggcggtt cagcgcctgc
    29281 aagatgtccg ggcgtaatac ttcaaagctg cgggtgataa acacctgcgc cgctacggtt
    29341 tcggtttccc gcactttggt acgtagcaga ttgagcaacg ccgcatcgcg cccgtggctg
    29401 gcttccggca ccagatgatc gtggtcgaga tagcgcagcg gctggtgaga aagtcgatgc
    29461 agatcttcgt cagaaagccc gacaatcgcc tggcagccca gcggttcccc cagcgcatcg
    29521 gcgcgcataa tgttgccaag acgcgaacgg atatccgcca gccacggctg ccacggttcc
    29581 gccagttcaa tttgcagcca cactgccagc gcaatggtgc tgtcgagaac cgcgcgaaaa
    29641 cccagacgcg gatcgctttt ggcgaccatt ttttccgccg acaggtgagt cagcgtatcc
    29701 ggcttttttg ccaccggctg gcggcacagt tcgcagcacg cctgcggatg ttcatcgcta
    29761 ctggtcagcc catgaacggg ctgcggctgc tgctgttcat cgtcaacaaa caggcggccc
    29821 tgctcgtcaa taaacttgat gcgcagatgg cggctttcca gcaactcccg ggcagagggc
    29881 gtcaggcgac tgtccgcagg gagatggatc tctgcgcctt cgctgagcgt atggttcgct
    29941 cttagccatg cttcggtgat gaaatctttc atagggattg ccagttagcc ggccaggcga
    30001 catacaggaa tttcacgctg gatgtcgtac caaattcgat gctggagcct ttcgggataa
    30061 acatcacgtc gcctgctttg gcgatcatgg tttcgccttc gtggcgaaca tggagctccc
    30121 cttccagcac catgtcgatt tcgtcgtagt tcagggtcca cgggaagaat gcgttctccc
    30181 actgcataaa accggcggcc atgctgctgc cgtcgtctcc ggtcacaaga tcagtcaagc
    30241 ccacgcaatg cggttgtgcg ccatcaaaac gaccaaactt gacgctgctg ccatcaatca
    30301 ctttgatgcc gcctttgccg gtcaccgatt tgaagctcgg ctgcattgcg ccctgctcca
    30361 gcgactgttt ttccttcatc actttttcca tcagctgtgc gaccaggctt tcggtaaact
    30421 ggccttccgg cagctgggca atgatggttt cgcggatgcg ctggctttcg gttttgtcgg
    30481 ctggcacgct ggcgggcacg gacgccgtta ccgggatcga ttcatcacat tcggtgatgg
    30541 taaaacccag tagatccgcc actttacggg cttccggggt aataatgctg gcgcgcagaa
    30601 ccacggacat tgcctgttcg ccgcgcgcgt gtgcttcacg aatatcattc gctgtgatga
    30661 gttttttcac ctgcttcctc ctgtttggtg agagatgcca gataatccac cagttgctgc
    30721 acgctttgcg ggtcgtggct gtttagctcg aaaatcggct cttcgaaacc cgtctcaagc
    30781 aataactttc gcgttgcggc gacgtcagca tctggcatgt ccgttttact gatgacggcg
    30841 atttgccgtt tgctgacgcc aatatccagc aacccggcag gcaaccgact ttccgggtca
    30901 tttgcgccat ggacatagat cagcatgtct acatcctgta gcgtggtaat taaggcgtga
    30961 taccagcggg gatggctaaa atattcaccc ggcgtatcaa tatcgccctt atcattaaat
    31021 tccacggcct gtgtttttct ggcgagggta taattcccct gcaacgcatt aaataacgtt
    31081 gtttttcctg ccccgacgct acccacaaac gcaattcgtt tcatgaatgc ctcgtattaa
    31141 cttttggtca tttcacaaag cgtataattt aataagcgac ctaaaccgct gacggtttgt
    31201 gataacgctt cttctactgc gcccaccgag ccataaatca ccaatgcacc gctgaagcgg
    31261 tcgagaaaac cgatatgcac atcagcggct ttcagcgcta aatcaccgac gatcatcgcc
    31321 gtttcgccgg gagttagcgt cataatgcca attacgcccg catccggaac gccgattttc
    31381 ttcgccagtt cctcaccggg atgcgcgatg agatgcgcca gtgtgacctg tttgccgggc
    31441 acaaattcct gaatgatgcg ttctttatcc atgagtcgcc gcctgtgaaa aactttcgtg
    31501 tatcgtgaca atttttcgtc agagtaaata acaaaattcg gcaagcgcgt ttaaaaggtg
    31561 aggtagatca ctaagaatgg agagatattc gctgtggtgc ataaactttc atatgcaacg
    31621 catgaatata aataaaataa tatcgagata ttatatagac tgcttttttt attattcgca
    31681 aactcagtaa taagctttac taatatattg cagccaaacc gcttaccctg aatattcagg
    31741 gtaagcgtga gagttaaaaa agaattacag cggttgggtt tgcgcttcaa ccacggccag
    31801 cgccaccatg ttgacgatac gacgcaccga tgcgatcggc gttaacacgt gaaccggttt
    31861 cgccacgccc atcagcaccg gaccgacagt cacaccttcc gagctggaaa cgcgcagtaa
    31921 gttgtaacta atgcgggcag cttccatgtt cggcatcacc agaatattgg cagaaccttt
    31981 caaagggctg tccggcatac ggtcgttgcg aatcgcttcc accagcgccg catcgccgtg
    32041 catttcacca tcaatcatca gttctggcgc acgttccctg accagttcca gcgcctgacg
    32101 cattttgctc gacgacgggc agtcagaaga accaaagttg gagtgcgaca acagagcaac
    32161 gcgcggttca ataccgaaac gacggacagt ttctgccgcc atcaaggtga tctccgccag
    32221 ctcttctgcg tctggttcgt cattgacgta ggtatcggca ataaaggtgt taccactcgg
    32281 cagcagcagc gcgttcattg cacctgcggt gtgaacgcca tcgcgataac caaagacatt
    32341 tttcaccacg ctaaaatgtt catgataatc acccaccgta ccgcaaatca ttgcatcggc
    32401 ttcgccacgc tgaaccatga tcgcgccgat cactgtcggg ttactgatca gcgcccgctg
    32461 cgcctgttcc tgagtgacgc cgcgacgctt catgatctgg aagtattcgg tccagtactc
    32521 tttaaagcgc ggatcggatt cgttattgac gatctcaaaa tcaacgcccg ctttgatctg
    32581 caagcccagt ttctgaatgc gcatttcgat cacgttcgga cgaccgataa ggatcggttt
    32641 cgccagtccc agcgttacca gttcctgagt ggcatgcaga acgcgcgcct cttccccttc
    32701 cggcagaact acgcgcttcg gcgctttgcg agcctgggag aaaatcggct tcataaacag
    32761 gttggttttg taaacgaact cagtcagctt gtcgatgtag acgtcgaaat cagcaatcgg
    32821 acgcgtcgcc acgcccgact ccatcgccgc tttcgcgacc gcaggagcga tcttaacgat
    32881 caagcgcgga tcaaacggtt ttggaatgat gtattccgga ccaaagctca gatcctgatc
    32941 gccatacgct gaagccacca cttcgctctg ttccgcatgg gccagttctg caatcgcgcg
    33001 taccgccgcc agtttcatct cttcgttgat ggcggttgcg ccaacgtcca gcgcgccacg
    33061 gaagatgaac gggaagcaaa ggacgttgtt gacctggttt ggatagtcag aacgaccggt
    33121 gcagatgatg gcatccggac gcacttcttt cgccagcggc ggcagaattt ccggttccgg
    33181 attcgccagc gccagaatca ttggtgcccg agccattttc ttcaccattt cctgggtcag
    33241 cactttcggg ccggaacagc ccaggaaaat atccgcacct tcaatcacat catcgagggt
    33301 acgtttgccg tcatccacca ccgcatatgc ggctttggtt tccgccatgt ttggctcacg
    33361 gccttgatag ataacgcctt ttgaatcgca aaccacgatg ttatgttttt gcagacccag
    33421 cgctaccagc agattcatac aggcgattgc tgcggcaccc gcgccggaaa ccaccatccg
    33481 cacgtcggag atgtttttct ccaccacgcg caagccgttg aggatggcgg cagtgctgat
    33541 gattgccgtg ccgtgctgat cgtcgtggaa taccggaata ttcatccgct cgcgcagttt
    33601 ctgttcaata tagaaacatt ctggcgcttt aatgtcttcg aggttgattc cgccgaaggt
    33661 cggttcgagc gcagcgacaa cttcaataaa tttgtccggg tcgagttcat caacttcaat
    33721 gtcaaataca tcaatcccgg cgaatttctt aaacagaacg cccttgcctt ccatcaccgg
    33781 tttgccagcc agcgcgccaa tgttgcctaa ccccagcacc gccgtaccgt tagagatcac
    33841 cgccaccagg ttaccgcggg cggtatattt gtaggctttt aacgggtctt tttcgatttc
    33901 aagacaaggt gcggcaacgc cgggtgagta ggccagcgcc agatcgcgct gcgttgccag
    33961 aggcttggtt ggagaaacct ggattttccc tggaactgga aattcatgga aatcaagtgc
    34021 actttgtttt aactggtcat ccatttggtt gttcctttca cgtaacgttc acaaataaag
    34081 tgtgtgggca acaccccctg cccacaacgt ggcgcacatt attaccttgc cggagtctac
    34141 agactttgag caagtccaaa ctctcaccat taatataatg ttttggtaat aatcctataa
    34201 cactgatgtt acctgcttaa tccagcaata ccatgcctgt ctgctatgct tttttgatgc
    34261 gtttagcgaa atttctcaga agtgtgaatt aacgcactca tctaacactt tacttttcaa
    34321 ggagtatttc ctatgaacga gttagacggc atcaaacagt tcaccactgt cgtggcagac
    34381 agcggcgata ttgagtccat tcgccattat catccccagg atgccaccac caatccttcg
    34441 ctgttactca aggctgccgg attatcacaa tatgagcatt taatagacga tgctatcgcc
    34501 tggggtaaaa aaaatggcaa gacccaggaa caacaggtgg tcgcagcgtg tgacaaactg
    34561 gcggtcaatt tcggtgctga aatcctcaaa atcgtacccg gtcgcgtgtc aacagaagtt
    34621 gatgcacgcc tctcttttga taaagaaaag agtattgaga aggcgcgcca tctggtggac
    34681 ttgtatcagc aacaaggcgt tgagaaatca cgcattctga tcaagctggc ttcgacctgg
    34741 gaaggaattc gcgcggcaga agagctggaa aaagaaggta ttaactgcaa cctgacgctg
    34801 ctgttttctt ttgcacaggc acgggcctgt gcggaagcag gcgtttttct gatttcgccg
    34861 tttgtcgggc gtatttatga ctggtatcag gcacgcaagc cgatggaccc gtatgtggtg
    34921 gaagaagatc cgggcgttaa atcggtgcgc aatatctacg actactataa gcaacaccac
    34981 tatgaaacca ttgtgatggg cgcgagcttc cgtcgcaccg aacaaatcct cgccttaacc
    35041 ggctgcgatc gactgactat cgcaccgaat ttgctgaagg agctgcagga aaaagtttcg
    35101 ccagtggtac gtaaattaat cccaccttct cagacgttcc cacgcccagc tcccatgagc
    35161 gaagcggagt tccgttggga gcacaatcag tatgcgatgg cggtagaaaa actgtctgaa
    35221 ggcattcgtc tgttcgccgt tgatcaacgc aaactggaag atcttcttgc cgccaaacta
    35281 taaaccagcc acggagtgtt atatgtcccg aaaagacctt gccaatgcga ttcgcgcact
    35341 cagtatggat gcggtacaaa aagccaattc tggtcatccc ggcgcgccga tgggcatggc
    35401 tgatattgcc gaagtgctgt ggaacgattt tcttaaacat aaccctaccg acccaacctg
    35461 gtatgatcgc gaccgcttta ttctttccaa cggtcacgcg tcgatgctgc tctacagttt
    35521 gctgcatctg accggttacg acctgccgct ggaagagctg aagaactttc gtcaactgca
    35581 ttcgaaaacc ccaggccacc cggagattgg ttatacgcca ggcgttgaaa ccaccaccgg
    35641 tccacttgga caaggtttgg cgaacgccgt cgggctggcg atagcagagc gtacactggc
    35701 ggcgcagttt aaccagccag accatgagat cgtcgatcac ttcacctatg tgtttatggg
    35761 cgacggctgc ctgatggaag gtatttccca cgaagtctgt tcgctggcag gcacgctggg
    35821 actgggcaag ctgattggtt tttacgatca caacggtatt tccatcgacg gtgaaacaga
    35881 aggctggttt accgacgata cggcaaaacg ttttgaagcc tatcactggc atgtgatcca
    35941 tgaaatcgac ggtcacgatc cgcaggcggt gaaggaagcg atccttgaag cgcaaagcgt
    36001 gaaagataag ccgtcgctga ttatctgtcg tacggtgatt ggctttggat cgccgaataa
    36061 agcaggtaag gaagaggcgc acggcgcacc gctgggggaa gaagaagtgg cgctggcacg
    36121 gcaaaaactg ggctggcacc atccgccatt tgagatccct aaagatattt atcacgcctg
    36181 ggatgcccgc gaaaaaggcg aaaaagcgca gcagaggtgg aatgagaagt ttgccgccta
    36241 taaaaaggct catccgcaac tggcagaaga gtttacccgt cggatgagcg gtggtttacc
    36301 gaaggactgg gagaaaacga ctcagaagta tatcaatgag ttgcaggcga atccggcgaa
    36361 aatcgccacc cgtaaggctt cgcaaaatac gcttaacgct tatggcccct tgctaccgga
    36421 gctgctcggc ggttcggcgg atctggctcc cagcaacctg accatctgga aaggttctgt
    36481 ttcgctgaag gaagatccgg cgggcaacta cattcactac ggggtgcgtg aatttggcat
    36541 gaccgctatc gccaacggca tcgcgcacca cggcggcttt gtgccgtata ccgcgacgtt
    36601 cctgatgttt gttgaatacg cccgtaacgc cgcgcggatg gcggcactga tgaaagcgcg
    36661 gcagattatg gtttataccc acgactcaat tggcctgggc gaagatggtc cgacgcacca
    36721 ggctgttgag caactggcca gcctgcgctt aacgccaaat ttcagcacct ggcgaccgtg
    36781 cgatcaggtg gaagcggcgg tgggctggaa gctggcggtt gagcgccaca acggaccgac
    36841 ggcactgatc ctctcaaggc agaatctggc ccaggtggaa cgtacgccgg atcaggttaa
    36901 agagattact cgtggcggtt atgtgctgaa agacagcggc ggtaagccag atattattct
    36961 gattgccacc ggttcagaga tggaaatcac cctgcaagct gcggagaaat tagcaggaga
    37021 aggtcgcaat gttcgcgtgg tttccctgcc ctcgaccgat attttcgacg cccaggatga
    37081 ggaatatcgg gagtcggtgt tgccttctaa cgttgcggct cgcgtggcgg tggaagcagg
    37141 tattgccgat tactggtaca agtatgttgg tctgaaaggg gcaattgtcg ggatgacggg
    37201 ttatggggaa tctgctccgg cggataagct gttcccgttc tttggcttta ccgccgagaa
    37261 tattgtggca aaagcgcata aggtgctggg agtgaaaggt gcctgatagt gatcgccgga
    37321 tgctgattgc cggatgcgac gctgacgcgt cttatccggc ctacagcgtc ttatccggcc
    37381 tacatgtccc cgccattttg tttaacgggt gatccacaac gtgggccagg cgtctggccc
    37441 atgccagtta tcgcaggtgg gttctgcggc gtaacgcacc aggcgaaaac gctgaccgtc
    37501 aaagcgccag cgcgcctgaa tgccacaatc gcttaatccg cgccctttcg ctaaggtcac
    37561 cagttcacgc gatttctcat caaatgttgc gttcatcagt tccagttcat tcgtctcctg
    37621 cccgttgttg aacggcaaac gcaaccgaac cgggcgcgag gctagcggct ttttacgcga
    37681 cacaatccat gccaaatcaa tggtgttata ggcccctgcc tcacagctga tcatcatcag
    37741 cgctttatca tcagtcagcg cagtgacatt cacctcacga cgtaacggat caagcgagca
    37801 gcgcagacca ttcatccgcc agtttccata atccagcaaa tcgttgcgtt cttcgagtga
    37861 gagtggcgtc ggcgttgggt taaccaccgc gacctctttc agcgcaggcg cgggcggtac
    37921 gctgagcggc ggttcgtccc ctttcttgat ccacgcggtt tcactgccaa cgcgcttttg
    37981 ctgagcatca ataaacaaca acgccgcttt taagccactc agagaaatgg tctgattgcc
    38041 gtcgcgtaag gtgatcgcct tcccttcctg aatcatctgc aaaaacgcgg tgatggttgc
    38101 cgtatcatcg gttactaata accatggtga aatccgccac ttgtcaccac ttaacgccaa
    38161 cggctcacca tctaacagca gccgtggcgc tatctctcct tcagacgcat ccggcgactt
    38221 caacccgccg cgctcaatac gtaaaacggc atcggtatgc gccccggcgc tgcggctcag
    38281 ggtcatcacc agtccattat gatcgcccgt attgcgcgcc acgcagaagt tttggttatt
    38341 acaggtgacc tgccagtcgg aaaacgcccg ttgcgctagt gccgcccaca ccaaagacgt
    38401 cggcaacaaa gcgaaaaaaa agagaaggaa aatgcgatag cgcatggacg gtacgacccc
    38461 agaagttcaa acaaacagca caagtcgtat cttcgttgcg caggtagcgc ggctcaatca
    38521 gatttgtcgg attgagccaa taataaacaa cgaaatatta ttgttatttt tcagtccatt
    38581 aaatgtgacg tttgcaaata gttaagcaat aacaccgtct taccgtcacg tatctcgccg
    38641 gttttgatca tctccagcgc ctggctgaac ggcagctcaa gcacttcaat atcttcatct
    38701 tcaacaccgc caccggcgtt agcgcgctga ttgtcactgt attcggcgat aaaaaagtgg
    38761 attagctcag tcacaccgcc aggcgacata tacagttcaa ataatttgcg cacttcgccc
    38821 acttcatagc ccgtctcttc aatcgcttct ttacgaatgc acacttccgg ttcgtcgtta
    38881 tccagcagcc cggcgcaggt ttcaatcagc tgcccgcttt cattgccatt aacccaggta
    38941 gcgacacgga actgacgaat cagaaccacg gtctttttct tcgcgttgta caggaggatc
    39001 gtcgcaccat taccgcgatc gtaaacttca cgtttatggc ggataacttc gccatctttg
    39061 cgcgtgagat cgtaagtaat gttgtgcagg gtgaaatagt tatcggagag aattttgtct
    39121 ttaatgaggg tgatttgttg cgtcataccg actccacagc gcgaaatgaa caattatatt
    39181 acgctgtgaa gtcgggtttg tctgcgcagg ctatcagtga gatttgactg attttacccc
    39241 cagccagtca ataatcccct gtgccgcatg acgtccttct gccattgcgg taaccaccag
    39301 atccgcacca cgcacggcgt cgccaccggc gaagattttc ggattggtgg tctggtaacg
    39361 gtactggctt tccacatccg cgatgatgcg gccccatttg tctaccgtta caccgtgcga
    39421 ctccagccac ggcatcccgt gtggattgaa gccaaacacc ataatcaccg catcggctgg
    39481 catgacaaat tcactgccct caatcggtac aagtcgccgg cgcccctggg catccggctc
    39541 gccaagacgc gtgcgcagga aacgaattcc gcaaacgtga ccttgctcat tcagctcaag
    39601 cgccaccggc tggacgttaa attcgaagtt ggccccctct tcgcgggcgt tcttcacctc
    39661 tttcttcgag cctggcatgt tagcttcatc acgacgataa gcgcaggtga cgttactcgc
    39721 gccgtggcgc agcgcggtac gcacacagtc catcgcggtg tcgccgccgc ccagtaccac
    39781 gacgttgagt ccggcggtat tgataaacgg ctcttccggt agctcttcga gtcccatcac
    39841 ctgtttagtg ttggcgatga ggaacggcag cgcgtcataa acgcccggcg catcttcatt
    39901 gggtaaaccc gctttcatgg aacggtaagt gcctacgcca acgaagaccg cgtcgtattg
    39961 ttccaaaagc gaatccaaag agacatcttt acccacttca caattgagtt cgaagtgaat
    40021 ccccatcgcg ctgaagattt cccgacggcg tgccagcagg gatttatcca gtttgaaaga
    40081 aggaatgccg aaagtgagca agccaccaat ttctggatgg cgatcgtaca ccgtcaccgc
    40141 cacgccattg cgggtcagaa catccgcaca ggccagccct gccggacccg caccgataat
    40201 cgccacccgc ttgtccactt tggtgacatg gcttaagtca ggacgccaac ctttcgccaa
    40261 cgcctgatct gaaatgtagc gttcaatgtt gccgatagtt accgcgccgt gctcatcgcg
    40321 aatagtacag gcaccttcac acaaacggtc ctgcggacaa acgcgtccgg taatttccgg
    40381 cagggtgttg gtcaggtgag aaagctcgac ggcggcgtcg atgtttccgg ctttcaccag
    40441 ttcaatccac tgcggaatat ggttatgcag cgggcaggtc cattcacaaa cgctgtgctc
    40501 gccgcattta aggcagcgcg aggcttcccg ttgtgcctga tcggcacgga atggcagata
    40561 aatttcatca aaaccggttt tgcgcgcttc aatcgccagt ttatccggct cgccacgcgc
    40621 gggcgttgcc tgcatttgtt cgactttact cattaccggc atttcttgtg cagcggtact
    40681 ggcatgccac ggttgatgct cctgacgtgc ggtgcgcaag cggcgggatt tcgccatgcc
    40741 ggagagtgcg acgtcagtga ccagttgcag cgcgtccgcc gggcaattct cgacacaggc
    40801 aggaccgttt tcgcgccctg cacaaaggtc gcatttatgc gccgtggctt ttacttttcc
    40861 tgccgcgacg ggtgtcagga cgatttgcat cgtaccaaac ggacaggcca ccacgcagga
    40921 tttacagcca atacactttt gctgattgac ctgaatgctg tcatcaacgt ggctgattgc
    40981 gccattaggg cagctacggg cgcagggcgc atcttcacaa tggtgacagg tcactgcact
    41041 acgttgctgc tgatgtttga taaccgtaat tcggggatga aaatggtgtt ggctcaggac
    41101 atgttgctca tcattgtgag ccatgacaca ggcgatttca caagcatgac aacccagaca
    41161 ttgctgactg ttggccataa taaaacgatt cataacgacc ttcttttttg gttgtaaaaa
    41221 ccttattctt tatatgagtg ttgttattac ccgacttaca ggggaatcgg caatgttcat
    41281 gtgcccagaa taagtaacta tttcgctata aactgtggca gatcaaataa tcccatcact
    41341 gactaaattg cgttttagcg aactggaaca ttaatgattt tttgtggaga agacgcgtgt
    41401 gattgttaaa cgacccgtct cggccagtct ggcccgggcc tttttttaca ttgtgctgct
    41461 gtcgattctt tccacgggta tcgctctgct aactctggcg agcagtttgc gcgacgctga
    41521 ggctatcaat attgccggat cgctgcgtat gcagagttac cgcctgggct acgacttgca
    41581 aagtggcagt ccacaactca atgcacatcg ccagttgttt cagcaggcac tgcattcatc
    41641 ggtattaacc aatctcaacg tctggtatgt gccagaagca gtaaaaactc gctatgcgca
    41701 tctgaatgcc aactggctgg agatgaataa tcggctcagc aagggcgatt tgccgtggta
    41761 tcaggccaat attaataatt atgttaatca gatagacctg ttcgtgctgg ctttacagca
    41821 ctacgctgaa cgcaaaatgc tgctggtggt ggcgatttcc ctggctggcg gtatcggtat
    41881 tttcacgctg gtctttttta ctttgcgccg tatacgccat caggtggttg ccccgctgaa
    41941 tcagctggtt accgccagtc aacgtattga acacgggcag ttcgactcgc cgccgctgga
    42001 taccagtctg ccgaatgagc ttggtctgct tgcaaaaacc tttaaccaga tgtcgagcga
    42061 gctgcataaa ttgtaccttt cgctggaggc gtcagtagaa gaaaagaccc gcgatctcca
    42121 cgaggccaag cgtcgtctgg aggtgttgta tcaatgttcg caggcgctga acacaagcca
    42181 gattgatgtg cattgtttcc gccatatttt gcagattgtt cgcgacaatg aagcggctga
    42241 atatctggag ttaaatgtcg gtgaaaactg gcggattagc gaagggcaac caaacccgga
    42301 attgccgatg cagattttac cggtgacaat gcaagagacg gtttacggcg aactgcactg
    42361 gcaaaatagt cacgtttcat catcagaacc gctgcttaac agcgtttcgt cgatgctggg
    42421 acgcggtttg tactttaatc aggcgcaaaa gcattttcag cagttattgt tgatggaaga
    42481 acgtgcaacc atcgcccgcg aattgcacga ctcgctggct caggtacttt cttacttacg
    42541 tatccagttg acgttactga agcgttcgat accggaagat aacgccaccg cacaaagtat
    42601 catggccgac ttttcccagg cgttgaatga tgcttatcgg cagttacgcg agctgttgac
    42661 tactttccgc ctgacgctgc agcaggcgga tctcccctcc gcgttgaggg aaatgctgga
    42721 tacgttacaa aatcaaacca gcgccaaact gaccctcgac tgccgtctgc caaccctggc
    42781 gctggatgcg caaatgcagg tgcatttgtt gcaaattatt cgcgaagcgg tgctgaatgc
    42841 gatgaagcac gccaacgcca gcgaaatcgc cgtcagttgc gtcaccgcgc cggacggcaa
    42901 tcacacggtt tatatccgtg ataacgggat tggtatcggt gaaccgaaag aacccgaagg
    42961 tcattatggt ctgaatatca tgcgcgaacg cgcggaacgg ctgggtggga cgctgacttt
    43021 ttcacaacct tccggcggcg gcacgttagt gagtattagc tttcgctctg cggagggtga
    43081 ggaaagtcag ttgatgtaat gcctcctact gaccaaagaa tacttgcact taaggttcag
    43141 tataaaaggg catgataatt tacattaact cctttttttc tccacgattg gctcgtacct
    43201 tgccgctaca gtgaagcaag tcaagcctac aacgatacgc agaaacacga ggtcctcttt
    43261 taatggcgaa tttctttatt gatcgcccca tttttgcctg ggtgctggca atcctgttgt
    43321 gtctgacagg gaccctggcg attttttcat tgcccgttga acaatacccc gatctcgcgc
    43381 caccgaatgt gcgagtgacc gctaactatc ccggcgcatc ggcccagacg ctggaaaaca
    43441 ccgtgaccca ggttatcgag caaaatatga ccggcctcga taatctcatg tatatgtcat
    43501 ctcagagcag tggcatcggt caggcatctg tcactttaag ttttaaagca ggcaccgatc
    43561 cggacgaagc cgtgcagcaa gtacaaaacc agctgcaatc agccatgcga aagttaccgc
    43621 aggcggtgca aaatcagggc gtgacggtgc gtaaaaccgg cgataccaat attctgacca
    43681 ttgctttcgt ctctaccgat ggttcgatgg ataaacagga tattgccgat tatgttgcca
    43741 gtaatattca ggacccgtta agccgcgtga atggcgtcgg ggatatcgat gcctatggtt
    43801 cgcaatattc catgcgtatc tggctggacc cggcgaaact caacagtttc cagatgacgg
    43861 ctaaagatgt cactgatgcc attgagtcac agaacgcgca gattgcggtt gggcaacttg
    43921 gtggtacacc ttccgtcgat aagcaggcgc tcaacgccac cattaacgcc cagtcactgc
    43981 tgcaaacacc agaacagttc cgcgatatca ccttgcgggt gaatcaagac ggctcagagg
    44041 taaggctggg cgatgtcgcc accgtcgaaa tgggggcgga gaaatacgat tatcttagcc
    44101 gcttcaatgg taagccagcc tccgggctgg gggtaaaact ggcctccggc gcaaacgaaa
    44161 tggcgacagc ggagctggtg ctcaatcgtc tcgacgagct ggcgcagtac ttcccgcacg
    44221 gactggaata caaggtggcg tatgaaacca cctcgtttgt taaagcctcc attgaagacg
    44281 tggtgaaaac gctgctggaa gctatcgccc tggttttcct cgttatgtat ctgttcctgc
    44341 aaaacttccg cgccacgctg atacccacta tcgccgtgcc ggtggtgttg atgggaacct
    44401 tctccgtact ttacgccttc ggttacagcg tcaacacctt aaccatgttc gcgatggtac
    44461 tggcgatcgg tctgctggtg gatgacgcaa tcgtggtggt ggaaaacatc gaacgtatta
    44521 tgagtgagga aggactcact cctcgcgaag ccacacgtaa atcgatgggg cagatccagg
    44581 gggcactggt cgggattgcg atggtgcttt cggcggtatt tgtaccaatg gccttcttcg
    44641 gcggcaccac cggtgccatc tatcgccagt tctctattac cattgttgcg gcgatggtgc
    44701 tgtcagtact ggtagcgatg atcctcactc cggctctgtg tgccacacta cttaagccac
    44761 tgaaaaaagg tgagcatcac gggcaaaaag gcttttttgc ctggtttaac cagatgttta
    44821 accgcaacgc cgaacgctac gaaaaagggg tggcgaaaat tctccaccgc agcctgcgct
    44881 ggattgtgat ttatgtcctg ctgcttggcg gcatggtgtt cctgttcctg cgtttgccga
    44941 cgtcgttctt accgctggaa gaccgtggca tgtttactac ctcggtacaa ttgcccagcg
    45001 gttcaaccca acaacagacc ctgaaagtcg ttgagcaaat cgagaaatat tacttcaccc
    45061 atgaaaaaga caacatcatg tcggtgtttg ccaccgttgg ttctggccct gggggtaacg
    45121 ggcaaaacgt ggcgcgaatg tttatccgcc tgaaagactg gagcgaacgc gacagtaaga
    45181 ccggcacctc gtttgccatt atcgagcgtg caacaaaggc gtttaacaag attaaagaag
    45241 ctcgcgttat cgccagcagc ccgccagcaa ttagcggtct tggtagttct gcgggttttg
    45301 atatggagtt gcaggaccac gctggagcag gtcacgatgc gctgatggca gcacgtaacc
    45361 agttgctggc gctggcggcg gaaaacccgg agctaacccg tgtgcgccat aacggcctcg
    45421 acgacagtcc gcagttgcag attgatatcg accagcgtaa agctcaggcg ctgggcgttg
    45481 ctatcgacga tattaacgac acactgcaaa ccgcctgggg ttcgagctat gtgaatgact
    45541 ttatggatcg cggtcgcgtg aagaaagtct atgtgcaggc agctgcgccg tatcgcatgc
    45601 tgccagatga catcaatctc tggtatgtcc gaaataaaga tggcggcatg gtgcccttct
    45661 ctgctttcgc gacctcacgc tgggaaacag gctcgccgcg tctggaacgc tataacggtt
    45721 attctgcggt tgagattgtt ggggaagccg caccgggggt cagtaccggt acggcgatgg
    45781 atattatgga atcgttagtg aagcagctgc caaacggctt tggtctggag tggacggcga
    45841 tgtcgtatca ggagcgactt tccggcgcgc aggctccggc gctgtacgcc atttccttgc
    45901 tggtggtatt cctgtgtctg gctgcgttgt atgaaagctg gtcggtgccg ttctcggtaa
    45961 tgctggtcgt gccgctgggg gtaatcggcg cgctgctggc aacctggatg cgcgggctgg
    46021 aaaacgacgt ttacttccag gtgggcctgt taacggtcat tggtttatcg gcgaaaaacg
    46081 ccatcctgat cgtcgagttt gctaacgaga tgaaccaaaa aggccacgac ctgtttgaag
    46141 cgacgctcca cgcctgccgt cagcgtttac gcccgattct gatgacctcg ctggcattta
    46201 tcttcggcgt attgccaatg gcaaccagca cgggtgccgg ttccggtggt cagcatgcgg
    46261 tgggtactgg cgtaatgggc gggatgattt cagccactat tctggctatt tacttcgtgc
    46321 cgctgttctt tgtgctggtg cgccgccgct tcccgctaaa gccgcgcccg gaataagcaa
    46381 taaaaaaggc gacatgccaa tgtgtcgcct ttttcaactt tccgattaag aacctgctca
    46441 gcgggttctt gctgtttata ctttgtctca ggaattactt acgaagcata acttcgataa
    46501 agtctttcca gttccccagt tcacgttcaa tcataacaac ctctcttata attatgggta
    46561 ttctacggaa acaatatacc gtggtgaagc taatttactc gattgctgcg atgactacct
    46621 ccggggggac aaatcttatg taaatactat ggtcctgcgg tgatgatttg tatgtgatac
    46681 acagcaacat ttcgagatat tcatacggca tctaatactg atttaattca ggttaaaata
    46741 cagacag
//
LOCUS       DADMKF010000010        45687 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.010, whole genome shotgun sequence.
ACCESSION   DADMKF010000010 DADMKF010000000
VERSION     DADMKF010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 45687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45687
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.010"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(48..1907)
                     /gene="gss"
                     /locus_tag="JNC24_000547"
     CDS             complement(48..1907)
                     /gene="gss"
                     /locus_tag="JNC24_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HAY9157388.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            2112..2978
                     /gene="yghU"
                     /locus_tag="JNC24_000548"
     CDS             2112..2978
                     /gene="yghU"
                     /locus_tag="JNC24_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HAY9157389.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPVSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETMNWLFWLQGAAPFLGGG
                     FGHFYHYAPMKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(3101..3349)
                     /gene="hybG"
                     /locus_tag="JNC24_000549"
     CDS             complement(3101..3349)
                     /gene="hybG"
                     /locus_tag="JNC24_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HAY9157390.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(3362..3703)
                     /gene="hypA"
                     /locus_tag="JNC24_000550"
     CDS             complement(3362..3703)
                     /gene="hypA"
                     /locus_tag="JNC24_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HAY9157391.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(3696..4184)
                     /gene="hybE"
                     /locus_tag="JNC24_000551"
     CDS             complement(3696..4184)
                     /gene="hybE"
                     /locus_tag="JNC24_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HAY9157392.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRSGE
                     NA"
     gene            complement(4177..4671)
                     /locus_tag="JNC24_000552"
     CDS             complement(4177..4671)
                     /locus_tag="JNC24_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HAY9157393.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNTPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(4671..6374)
                     /gene="hybC"
                     /locus_tag="JNC24_000553"
     CDS             complement(4671..6374)
                     /gene="hybC"
                     /locus_tag="JNC24_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HAY9157394.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(6371..7549)
                     /gene="hybB"
                     /locus_tag="JNC24_000554"
     CDS             complement(6371..7549)
                     /gene="hybB"
                     /locus_tag="JNC24_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HAY9157395.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLKRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIVLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(7539..8525)
                     /gene="hybA"
                     /locus_tag="JNC24_000555"
     CDS             complement(7539..8525)
                     /gene="hybA"
                     /locus_tag="JNC24_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HAY9157396.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(8528..9646)
                     /gene="hybO"
                     /locus_tag="JNC24_000556"
     CDS             complement(8528..9646)
                     /gene="hybO"
                     /locus_tag="JNC24_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HAY9157397.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEEGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(9835..10122)
                     /locus_tag="JNC24_000557"
     CDS             complement(9835..10122)
                     /locus_tag="JNC24_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 domain-containing protein"
                     /protein_id="HAY9157398.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(10241..11128)
                     /locus_tag="JNC24_000558"
     CDS             complement(10241..11128)
                     /locus_tag="JNC24_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HAY9157399.1"
                     /translation="MPRLTAKDFPQELLDYYDYYAHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYIMYPSPNGHGEVRGYLVKPAKMSGKTPAVV
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQAAGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHFAELDTRINEGWPAYEAALKANNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            11285..12325
                     /gene="gpr"
                     /locus_tag="JNC24_000559"
     CDS             11285..12325
                     /gene="gpr"
                     /locus_tag="JNC24_000559"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HAY9157400.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRIGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMHREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(12365..12859)
                     /locus_tag="JNC24_000560"
     CDS             complement(12365..12859)
                     /locus_tag="JNC24_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HAY9157401.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            13050..13934
                     /gene="yghA"
                     /locus_tag="JNC24_000561"
     CDS             13050..13934
                     /gene="yghA"
                     /locus_tag="JNC24_000561"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HAY9157402.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPVPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASIITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQKTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(14094..14519)
                     /gene="exbD"
                     /locus_tag="JNC24_000562"
     CDS             complement(14094..14519)
                     /gene="exbD"
                     /locus_tag="JNC24_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HAY9157403.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(14526..15260)
                     /locus_tag="JNC24_000563"
     CDS             complement(14526..15260)
                     /locus_tag="JNC24_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbB"
                     /protein_id="HAY9157404.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            15512..16699
                     /gene="metC"
                     /locus_tag="JNC24_000564"
     CDS             15512..16699
                     /gene="metC"
                     /locus_tag="JNC24_000564"
                     /EC_number="4.4.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HAY9157405.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFDCVEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCVLFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLSNEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            16839..17498
                     /locus_tag="JNC24_000565"
     CDS             16839..17498
                     /locus_tag="JNC24_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HAY9157406.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(17538..18437)
                     /locus_tag="JNC24_000566"
     CDS             complement(17538..18437)
                     /locus_tag="JNC24_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003024513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAY9157407.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVDYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            18631..19794
                     /gene="yqhD"
                     /locus_tag="JNC24_000567"
     CDS             18631..19794
                     /gene="yqhD"
                     /locus_tag="JNC24_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HAY9157408.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKI
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANDVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRETKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            19899..20726
                     /gene="dkgA"
                     /locus_tag="JNC24_000568"
     CDS             19899..20726
                     /gene="dkgA"
                     /locus_tag="JNC24_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HAY9157409.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKAPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGEIAKLDQGKRLGPDPDQFGG"
     gene            20926..21852
                     /locus_tag="JNC24_000569"
     CDS             20926..21852
                     /locus_tag="JNC24_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HAY9157410.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTVEQTVRQIYQNYKSDASTPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     ADHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPEAFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            21903..22160
                     /gene="yqhH"
                     /locus_tag="JNC24_000570"
     CDS             21903..22160
                     /gene="yqhH"
                     /locus_tag="JNC24_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HAY9157411.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDIKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(22203..24422)
                     /locus_tag="JNC24_000571"
     CDS             complement(22203..24422)
                     /locus_tag="JNC24_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HAY9157412.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSPRLDTPGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VTVQPPRPKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCVRALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLHCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKKPLAKIGYK
                     SEDVFVPKGDKQRRLHKALLRYHDPANWPLIRPALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(24533..25945)
                     /gene="ftsP"
                     /locus_tag="JNC24_000572"
     CDS             complement(24533..25945)
                     /gene="ftsP"
                     /locus_tag="JNC24_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HAY9157413.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYDVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(26020..26757)
                     /gene="plsC"
                     /locus_tag="JNC24_000573"
                     /gene_synonym="parF"
     CDS             complement(26020..26757)
                     /gene="plsC"
                     /locus_tag="JNC24_000573"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HAY9157414.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(26990..29248)
                     /gene="parC"
                     /locus_tag="JNC24_000574"
     CDS             complement(26990..29248)
                     /gene="parC"
                     /locus_tag="JNC24_000574"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001639862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HAY9157415.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKVAVKGKSNQSVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASNGDSEE"
     gene            complement(29794..30276)
                     /locus_tag="JNC24_000575"
     CDS             complement(29794..30276)
                     /locus_tag="JNC24_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAY9157416.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            complement(30329..30721)
                     /gene="ygiW"
                     /locus_tag="JNC24_000576"
     CDS             complement(30329..30721)
                     /gene="ygiW"
                     /locus_tag="JNC24_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HAY9157417.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            30873..31532
                     /gene="qseB"
                     /locus_tag="JNC24_000577"
     CDS             30873..31532
                     /gene="qseB"
                     /locus_tag="JNC24_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator QseB"
                     /protein_id="HAY9157418.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLILTARDALAERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            31529..32878
                     /gene="qseC"
                     /locus_tag="JNC24_000578"
     CDS             31529..32878
                     /gene="qseC"
                     /locus_tag="JNC24_000578"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     QseC"
                     /protein_id="HAY9157419.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNAHSIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVVDVTLNADNFIVRDNGPGV
                     TPEALARIGERFYRPPGQTATGSGLGLSIVQRIAKLHGMNVEFGNAEQGGFEAKVSW"
     gene            32988..33569
                     /gene="mdaB"
                     /locus_tag="JNC24_000579"
     CDS             32988..33569
                     /gene="mdaB"
                     /locus_tag="JNC24_000579"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HAY9157420.1"
                     /translation="MSNTLIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYDVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            33600..33914
                     /locus_tag="JNC24_000580"
     CDS             33600..33914
                     /locus_tag="JNC24_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HAY9157421.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(33962..35854)
                     /gene="parE"
                     /locus_tag="JNC24_000581"
     CDS             complement(33962..35854)
                     /gene="parE"
                     /locus_tag="JNC24_000581"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HAY9157422.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPNSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(35883..36464)
                     /gene="yqiA"
                     /locus_tag="JNC24_000582"
     CDS             complement(35883..36464)
                     /gene="yqiA"
                     /locus_tag="JNC24_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HAY9157423.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(36464..37291)
                     /gene="cpdA"
                     /locus_tag="JNC24_000583"
     CDS             complement(36464..37291)
                     /gene="cpdA"
                     /locus_tag="JNC24_000583"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HAY9157424.1"
                     /translation="MESLLTLPLAGEARVRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(37316..37738)
                     /locus_tag="JNC24_000584"
     CDS             complement(37316..37738)
                     /locus_tag="JNC24_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HAY9157425.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(37739..38368)
                     /gene="nudF"
                     /locus_tag="JNC24_000585"
     CDS             complement(37739..38368)
                     /gene="nudF"
                     /locus_tag="JNC24_000585"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HAY9157426.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            38573..40054
                     /gene="tolC"
                     /locus_tag="JNC24_000586"
     CDS             38573..40054
                     /gene="tolC"
                     /locus_tag="JNC24_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HAY9157427.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLAREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            40202..40873
                     /locus_tag="JNC24_000587"
     CDS             40202..40873
                     /locus_tag="JNC24_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HAY9157428.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            40879..42039
                     /locus_tag="JNC24_000588"
     CDS             40879..42039
                     /locus_tag="JNC24_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HAY9157429.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKRTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDDIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFIE"
     gene            complement(42077..42892)
                     /gene="ygiD"
                     /locus_tag="JNC24_000589"
     CDS             complement(42077..42892)
                     /gene="ygiD"
                     /locus_tag="JNC24_000589"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311954.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HAY9157430.1"
                     /translation="MTPLVKDIIMSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMT
                     LPRPQAIVVVSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPVLAQHLV
                     ELLASVPVTLDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLA
                     ALRDEGIMLVASGNVVHNLRTVKWHGDSSPYPWAMSFNEYVKANLTWQGAVEQHPLVN
                     YLDHEGGALSNPTPEHYLPLLYVLGAWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            43008..43781
                     /gene="zupT"
                     /locus_tag="JNC24_000590"
     CDS             43008..43781
                     /gene="zupT"
                     /locus_tag="JNC24_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HAY9157431.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQPLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(43839..44009)
                     /locus_tag="JNC24_000591"
     CDS             complement(43839..44009)
                     /locus_tag="JNC24_000591"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9157432.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(44270..44923)
                     /gene="ribB"
                     /locus_tag="JNC24_000592"
     CDS             complement(44270..44923)
                     /gene="ribB"
                     /locus_tag="JNC24_000592"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HAY9157433.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(45027..45175)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            45237..45587
                     /gene="ubiK"
                     /locus_tag="JNC24_000593"
     CDS             45237..45587
                     /gene="ubiK"
                     /locus_tag="JNC24_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HAY9157434.1"
                     /translation="MTYPYRTTMVLNTYQYRETTMIDPKKIEQIARQVHESMPKGIRE
                     FGEDVEKKIRQTLQAQLTRLDLVSREEFDVQTQVLLRTREKLARLEQRMSELENRSTE
                     IKKQPDPETLPPTL"
BASE COUNT        11280 a        12254 c        11465 g        10688 t
ORIGIN      
        1 cgtactgatt attcttcata atcagtacgg ggttgctaca acatgtatta ctttttcacc
       61 acaattaacg gttcaatatc actctctttt ttgatgacca gtgattcatc accgcgcaaa
      121 cacgtcccac cgtagttgcc gcccacggtg aaggtacata cctgaatgta tttaccgtcc
      181 actttcggca aacaccacag ttgctgatag atgtttttct gctcggcaaa tttaccgctg
      241 gttttgtcca gcacctcttc atgatggctg accaggtcga tattgctacc acagcgaccg
      301 gcaatcggtt tcactgcgta acctgttttc accaattcat cattaacagt gaaatcggta
      361 tccagcaggt aacgatggtg cgggaacagc gaccagagga tcggcagaat cgctttgttg
      421 ccggggatca ccgtccacag cggctcaaag actagcactt ccgggcgcag caatacgtcg
      481 ataagacgca cttcgttttg cggatgaccg gtacggattg gcaccgcagc aaactcacgg
      541 tcgctaactt cacgaatctg atcaaacgcg gtttcccacg cccaggtttt ccacacgcag
      601 ttaaccagtc gcccttcccc atcaatcagt tgcccggcag catcccagcc cagttcatcc
      661 aatccacgca agatacgcgt ttcaaagccc gcctggtgca gcgcctgctc cataaactgc
      721 gcgtgatagt tttcctcgat atctttgtcc tgcatgatat ggacaaacgg acgtgcacga
      781 ctgtgtttcc aggcaccagc caattcgtta atcagccctt ccgccggatt gaagccgttg
      841 cctttatagc cctgctccgc ccaacgttcg aggatcaagc ccgcttcggt atgacaggag
      901 gcggagtcgg cgttgtactc gtaaaccttc aggccacgct catccatgca gaaatccata
      961 cgaccagtga tcatatggtg acggcgacgc tgccaggaga gacgcaaacg tggccagagg
     1021 attttcggga tgtcgaacag cgccagcagg ttgtcatctt tcagcacctt gtcggttgcg
     1081 tgaagataca tcaggtgcag ctcgttggtg gctttaatta gctcctgctc ggcactctcg
     1141 gtaatggtgt agtaatgata aggatcctga ttgatcacct gaccgttggc ctgcacatag
     1201 gcgttttgca gcggatcttt ttcatccagc catttaccgt caaactggcc tttgttttcc
     1261 aggcgcgctc cgctgatttt cagcagctcg cctgcaattt ccggctgcgg taagctgtat
     1321 tcagtatctt ccgtctggat catccagccc agaatggtgg tgtcatcaaa agtgtctttc
     1381 agggtatagc agccgttttc gaccaccatc tccagctcac gcgtccactg ttgcccttgc
     1441 ggcaacgggg aatgaatcac gttctgttcc gcaatacgga ctttgttgcc atgcaattgg
     1501 gtaatgatgg cgacatggcc agtgtcttta aattcaccgc ctttatccca gataagaagc
     1561 gcacccgcga ccggcgcacg cggcgagccg ttaggaaatg cctgcaatgg caggatgttg
     1621 tcattaacca cttctcgcag gaagcgcagc gagaaaatct cccacgccat acccacgtca
     1681 gtaaagacca caccgtaatt cagaaagaga aaacggcgag caaattcaac gcattgccac
     1741 ttgtggccca tatattcgtc gtcgatatag ctacggaata cggcgtcatc ttcgtattcc
     1801 tgcggatcga gagaactgta atctgaagag tagattgcta ccccacctgg ggcgtagccc
     1861 aataatgtcc cgaacggggc atcctggctg gtcgttcctt tgctcatcac tttaccttta
     1921 acaatacaac ctaagcagtt ggcgtaattg ttgtgctctg attacctgct cagcgacatt
     1981 aaccggacag aggtcagact aatcatacac ctcatcgcag cggctggcgc aaagggttaa
     2041 aaaattcaca ttctgtacag caagtgacct gctacactgc ttcaacacta ccactcagaa
     2101 ggcaactcac tatgacagac aatacttatc agcccgcgaa agtctggacg tgggataaat
     2161 ccgctggcgg cgcgttcgcc aatatcaatc gcccggtttc tggtccgacg catgaaaaaa
     2221 cgctgcccgt tggcaaacac ccattgcaac tttattcgct gggaacgccg aacggtcaga
     2281 aagtaacgat tatgcttgag gagctgctgg cgctgggcgt tactggtgca gagtacgacg
     2341 cctggctgat tcgtattggc gatggcgatc aattctccag cggctttgtc gaagtgaacc
     2401 caaactcgaa gatcccggcg ctgcgcgatc atacgcataa tccgccgatc cgcgtgtttg
     2461 aatctggttc gatcctgctt tatctggcgg agaaatttgg ctacttcctg ccgcaggatt
     2521 tggcaaagcg tactgaaacg atgaactggc tgttctggtt acagggcgcg gcaccgttcc
     2581 tcggcggtgg ttttggtcac ttttaccatt acgcgccgat gaaaattgag tacgccatca
     2641 accgctttac catggaagcc aaacgtctgc tcgacgtgct ggataagcaa ctggcgcagc
     2701 ataagtttgt tgcgggcgat gagtacacca ttgcggatat ggcgatttgg ccgtggtttg
     2761 gcaatgtggt gttaggcggc gtgtatgatg ccgcagagtt tcttgatgcg ggcagttata
     2821 agcatgtaca acgctgggcg aaagaagtag gcgaacgtcc ggcggtgaaa cgtgggcgta
     2881 ttgttaaccg caccaacgga ccgctgaatg agcagttgca tgagcgccat gacgccagtg
     2941 atttcgagac gaatacggaa gataagcgtc aggggtaagg gttggtgttc gtcgcagcaa
     3001 gccatccagg ccggataagg cgttcacgcc gcatccggca atcgtgcaca atgcctgatg
     3061 cgacgctgcc gcgtcttatc aggcctacga aagctaatca tcacgcgctg gtaatgtcgt
     3121 aatccatttg gcgcagtgcg tctaatgtgg ctttggcttc atcttcgtcg atgatgctca
     3181 tggcaaatcc gacgtgtacc agcacccact ggcccagtag atcggcaggg ttaccttcac
     3241 aaatcagggc gatattcacg tcgcgcttga taccacatac ttcaacctgc gcaagctggt
     3301 gaatatcttc accgacagcc agcacctggc ctggaacgcc aatacacatt attaactccg
     3361 gttattcaac ttcaatactt ttgacgatca gcgaatcgcc ggtatcgaca cgcaaccgct
     3421 cgccgtgaca gagcggacac tgcgcatcgt gctgatgaat ctccaccacc tggctgcaat
     3481 cccagcacca agcctgggcg ggtttataga cgatatgtaa atcgcaccct tgcgccaccg
     3541 ttccgtggca gacaatttca aaactaaaac ggacggcgct ctcctcaacg caggagagcg
     3601 cgccaatttc cagccacacg gcggtgacgc gcttaacatc gtgctgctcc gcctgccgtt
     3661 ggataatttc aacggcgctc tggcaaagag acaactcatg cattttcgcc actcctgcga
     3721 ccaaacagca gggcgcgacg ccctgcgtgt ggtacatccg gattcgtgac tggcagcgaa
     3781 aggatcattc gtgcgcagtc atccgtcagg cgttggccct cttcaatcga catgctgtgc
     3841 gaaagcggcg acatcagcga acaggagaga tattgcgaaa caccgtccag ttcgccaacg
     3901 gtaaaggtca tagtgccata cggcagttgc agaccaattt tttcactcac tttgcgcagc
     3961 ggccagagtt gatccgggcc ggggaagata actgcactca gcatccacgg ggtgatcaca
     4021 caccccgtcc actgaccttc gaacagcgta aaatcagaaa catacaccgg cattgaagga
     4081 tgcagaaaag agagatcgtg catcgaacgc cgggcaattt cttcaaacgc tgcctgtact
     4141 tgcgccttcg gggaggtctg gaaacctgct atctcttcag tcatgaatcg cctcccgtgg
     4201 gatggcttcc acgcctgatt cgcgcagcgc agccagaacc tgctcaagcg caggttcaat
     4261 cattgcttca accgtcggcg ttaagccgat gtgtggctcc agcgattccg ggatcacgcc
     4321 gaccagggtc agctttttcg gaaactcacc ggtgaagcgc agggccgaca atacgtcggc
     4381 caggccaagc tgatgcggag agattttgtt ggtaaacaac gccggaactt cttcatcccg
     4441 caggatcatc atcgttcccg gcgtgttctt tttcgacaca atggcatccg caataatcag
     4501 atgatcgcga tttgccatgt cgccaagcag ctccattccc gccgtgccgc catcgaggat
     4561 ctcaacataa tccggcagaa tgtatcgttg ctctaacgct tcgacaatcc gcacaccgat
     4621 ggcttcatcg gtcagcaaaa tattgccgac ccctaagact aaaatacgca ttacagaacc
     4681 ttcactgaaa ccacttcgtt gccgtcggca tccactacgt gtaccgcaca ggccatgcac
     4741 gggtcgaagg agtgaatggt acgcaccact tccagcggtt tattcggatc agcaaccggt
     4801 gtacccacca gcgactgctc gtaaggaccg acgtcatcat tgaagttacg cggaccagag
     4861 ttccaggttg atggaacaac tgcctggtag ttactgataa taccgtcttt aataaccatc
     4921 cagtgagaga gcataccgcg cggcgcttcg aggaagccaa cacctttgaa ctcacccgtt
     4981 gccggaatgt tcggtttcac aaaggtggtg tgatcgcctt tgccgatatt ggtgatcagt
     5041 gcactgtatt gattttgcag gatatcctgc aattcgcagc agtgaacggt acgaccaata
     5101 atacggccca gcgtggagtg cagctgcgcc acttccagcg tgttgccagt cagtttctga
     5161 taaatcgcaa cgatttcatt cagtttgttc tgggtagatt cgcgacctgc cgccagtttc
     5221 accagcatat tagccagcgg acccacttct accgttttgc cgtagaaagt cggtgatttc
     5281 acccaggaat atttcccgtc gtcagaccaa ccatcataag ccggaatggt ggtaccttcc
     5341 cacggtgcct gcggcgcttc gtctttatac caggagtgct tcgcgctttc ctgaatgcct
     5401 ttgatcaggt attcatcgga atgagaagtg atcggacgat acgaggacag atccgcattc
     5461 tcaatgtagc cgcccgggaa caggaagctg ccgtttttac tgtcggtcgg gaattccggc
     5521 acgctcaggt agttcaccgc acctttaccg cgcgtcagcc attccgggta gaacgcggca
     5581 ataactgcgg tatcaacctt ataaacctgt tcaacaaagt cgctcagctt gtcgatgaaa
     5641 gacttgatgt acatcaggcg ctcaaggttc agcacgccca aaccgtcgag gttgatcggg
     5701 ttcgcgacac cacctaccgc caggttctga atgtgcggcg ttttaccgcc cagcagcgcc
     5761 acgacgcggt tagcgtcacg ctggcactcc agcgcctgca ggtagtgcgc taccgcaatc
     5821 aggttcactt ccggcggcag tttcatcgcc gggtgacccc agtagccatt ggcgaaaata
     5881 cccaactgac cgctggcaac cagatctttg atcttgttct gaactttagt gaactcttcc
     5941 ggactgttca gatgccaggt cgaaacgcct ttcagcattt cggaggcttt ggttgggtca
     6001 gcttgcagtg cagaagtgat gtccacccag tccagcgccg aaagctgata gaaatgaacg
     6061 atatggtcat gcgtggtgtg cgcagccaga atgatgttac ggatgtattg cgcgttaacc
     6121 ggaacgtcga tattcagcgc actttctgcc gcacgaacgg aagacagcgc gtgagtggta
     6181 gtacatacgc cacagatacg ttgcacaatc atccatgcat cgcgcggatc gcggtttttc
     6241 acgatctctt ccatgccgcg ccacatggta ccggaagccc atgctttcga aacgacgcca
     6301 ttttcgattt cgcaatcgat gcgtaaatgc ccctcaatac gggttaccgg atcaatagta
     6361 attctctggc tcatgctttg ctcgcctcat gacgattatg atcgttttgt tttaaaggag
     6421 gaagtatcgg cagtagacga atgagtacga tgtaagcgca aatctcaata gccacaaaac
     6481 caatagaaat caacagttct tcccaggtcg ggaagtaggc gtaaccgccg cccgggttga
     6541 atgccaccag cgaataggtc agacgccagg ttgcacaacc taacagtgcg ctcagtgctg
     6601 acaggaacag catgcgggaa tcattacgca gcttcgccac acgcagaacg accagcggga
     6661 agagcatcag caggacttca atccagaaca tcacggagta gaagtcaccg gcaaacgcta
     6721 acgacagctt gtcgcgatag ataagctcgc caaagcgcag cacgatgaaa atcgccagca
     6781 acacactgat ggtgttggtc agcttgacga acagactctt ttcatccgga ccgttgccac
     6841 gcagacccgc ctgcaccagc gaaccttcaa agatgacaat cgagaagccc atgatgaacg
     6901 ccgtcagcag cgagaacagc ggcaacattt catagctctg ccacaacgga tgcaccttgt
     6961 agcccgccga gatcatcagt gaccccattg aagactggtg catggtcggc aacagcgcgc
     7021 caagtgcaat gatgaagaac ataaccttgt tcagacgctt gagagaaacc ttccagccca
     7081 gacgttcaaa cagtgccgga gcaaactcca gtgccatcac gccgatatag atggtcatac
     7141 agaccgccgt ctcgaacagt accgagttca cgttgaagtg acccggaatg tagaagtacg
     7201 gcaggttcca gtagcgaccc acgtcgatag tgatcgacaa gccacccagt gagtaaccaa
     7261 acagactcgc caacagcgcc ggacgcacca gcggatggta ttgcccacgg ttaaagacgt
     7321 ataccgccca cgccagcgcc cagccgccac aggcaaagcc ggtgccaatc agcaggtcaa
     7381 acgcgatcca cacgccccac gggaagccgc cgttcaggtc agagacagag cccagaccga
     7441 acaccagacg cttcacaatc aggagcatac agatgacgat taacggtcca aaaatcatga
     7501 ccggtttact gatgattttg ccgcccagcg gttgtggatc atgactcatg atcgtctcct
     7561 ccgtcgtgat ggtcgttttt ggtgttgcga cgaaccagca cggttaagcc cgccagcaca
     7621 gccagtggta gcatcatgcc tttatacagg gtgtgttgaa tattttcgga acgcgcaccg
     7681 gtagaaagat cgtccagttt cggcaggtcg agattttcat aaggcacacc cgtcagtacc
     7741 agaacctgag taccgccgcc ctctttctcg ccgtacagat gcggataata tttcggcacc
     7801 gtatgcaggt aagtgtcgcc agatttcagc gtctgacgcg gatagtggta ttcgctgcca
     7861 ggcttcagcg ccagacgttt tttcgcctcc gccatcagct cttcacgcgt accgaaaatc
     7921 accgcgcccg ccgggcacac ttctacgcag ccaggcagac cgcctttatc gagacgttcc
     7981 acacctttct ggttgcacag ctcgcactta tgcagcgcac caaacgggtt gttgtagtcg
     8041 tacttcggca cgttgtacgg acaggcgacc atgcagtaac ggcaaccggt gcacacgtct
     8101 ttgtcgtaat ggacaatgcc ggttttcgga tcttttttca gtgcagagac cgggcacaca
     8161 gagacacagt tcggatcgac gcagtgcata cactgtttct taatgtacgc gtagccgttc
     8221 tcctcctggt ctttgttgac ccctgtgccg ctggtccaca cctgaatgat gttattggta
     8281 tacggcgaca gtttgtcgtt gttcgaccag gtctgttccc cttgcgggtt acgttcaggg
     8341 aagttgatat cctgacactt ggtgacgcaa gcctggcagc ctacgcacaa ggtcgagtcg
     8401 tacaacatcc ccagcgatcc cggaattggc gggcggtttt cagcagccgc atgactgaca
     8461 gacggcagcg cgcccgtcag caatgccccg caggaggctg ctttaataaa attacgtctg
     8521 ttcacggtta ttctccccgt gagtcagcgt tatctttctt ttgctgacga cccagttcac
     8581 gcaccgccat cacgctgaca ccggcaacca gcccaaccac accgccgagc aaaccaatag
     8641 cgcctgcaga gacgttgccg ccctctttag cgttaacatc cggtttctgt gaacgcggag
     8701 tctgattttc gacgttggca agctgatgga tgcctttatg gaagccgata ccttcttcgt
     8761 tacagccata gcatgggtga ccaatcgcta ccggccacac gccgccaaca tcgcagaatt
     8821 gcagcgttga gcagttgccg taagtttctg gccctttaca gccgaggtgg tacaggcacc
     8881 agccttcacg gtggccttca tcaccgaact ctttggcaaa acgaccagca tcgaagtgcg
     8941 ggcgacgttc gcagtgttcg tgaatcagac ggccataggc gaaggtcgga cggtttttgt
     9001 catccagttt cggcggtttg ccgtaagtga tgatgtgcgc aacggtcgcg aggaagttgt
     9061 gcgggttcgg cgggcagccc ggaatattga taacggtttt gcctggcaga acttcttgca
     9121 ggctgactgc gccagttggg ttaactccag ctgcggcaac accgccccac gcagagcagg
     9181 aaccgatagc gataatcgct gctgcgcctt ctgccgcttt gcggatgtga tccacaatcg
     9241 gctcaccagc aaccatgcaa taaataccgt tatcttttaa tgggatggaa ccatccacaa
     9301 ccaacacata ctgccctttg tacttctcaa gtgcgttatg tttgttctct tcgacctgat
     9361 gaccgaaggc ggcggaaagc acttcgtgat actccagaga gatagtctcc agcacgaggt
     9421 tttctaccgt tggatgcgtt gcacgaagca gagattccgt acaaccggtg cactcctgcg
     9481 cgccaatcca gataactggc ggacgctgcg ggttagtaac cgattcggcc atctctgcag
     9541 cggctttgct acttaacccc atggtggcgg ctaatgctgc acaaagcttc atgaaatcac
     9601 gacggttaat gccgtgagaa tggatgaggg tgttatctcc agtcatttat agttattccg
     9661 ttgcgaagac ctggcatata ttttgcctca atcgcaaaat caataatgcg atcgatgcgc
     9721 catttaccac acatttatta tggttatcga tatcatgata ctgcggcggg gcgctaaaac
     9781 gaagggaaga agcattagtg taattaatta aagcaagata atacgtatgt ttgatcaatt
     9841 ttcgaggcga taaccggcca taaaaaagcg caacgtagaa caggaattat tctcacgaaa
     9901 gaaatccatt accatctctt tatccagtcc atagcggcgc gtcagaatac ccgcttccca
     9961 ggcgctaagc tggcgatctc cggtcttttc gtacatccgg tgtgagtagc ctaatacaaa
    10021 accgcgttta taatcggcac agaattttgt cacattaacc gcactgtcgg catgcgttgc
    10081 ttttaatccc gccattaagc ctttaccaaa atggttattc atcgttcacc tcattcgcta
    10141 tatatcgcca tatatagcga tattttaggc aaagaacgac ttttgcgcac gctatttttt
    10201 catgcttgtt taagacgtaa tccagtaaga gataaaccta tcaggagaga tatttatcga
    10261 accatttcag tgtcctttgc caggcaagat cggcggcaga tttgtcataa cggggcgtgg
    10321 aatcattatg gaatccgtga ttaacccccg gatagatata cgcctcataa accttattat
    10381 tggctttcaa cgccgcctcg taagcaggcc agccctcgtt gattcgggtg tccagttccg
    10441 cgaagtggag tagtaaaggc gcttcaatct tcgccacatc ggcagtgggt gcctgacgac
    10501 cataaaacgg caccgcgcag gccagttccg gatacgcgac agccgccgcg ttcgatacgc
    10561 caccgccata gcaaaatccg gtaataccca ctttgcctgc cgcttgtgga tagcgttgca
    10621 taaactcaat tgcggcaaag aaatcattca tcagtttggt tggatcaacc tgctgttgca
    10681 gctcacgacc tttatcatca tttcccggat aacctccaac ggaacttaag ccgtcaggtg
    10741 ccagggcgat ataccccgcc ttcgccactc gccgtgccac atcttcgata tacggattca
    10801 gtccacgatt ctcatgcacc accaccacgg ctggcgtttt gccgctcatt tttgcgggtt
    10861 tcaccagata accccgtacc tcgccgtgac catttggcga aggatacatg atgtactcag
    10921 caacaatctc cgggtcggta aactctacct gagtcgccag cgcataattt ggcttgagca
    10981 aatcaaacaa cgctaatgcc gtcatcccgc ccaccgcata cttcgccgca aggttgagga
    11041 actcacgttt cgaaattttc ccgtgagcgt aatagtcgta gtaatccaac aactcttgtg
    11101 ggaaatcttt ggcggttaaa cgcggcatcg ttgcactcct cagttggtgt ttttttaagc
    11161 aaagcataag cacgtgtttt tgcccagttt ttcgtcactc tgtgagccag actacgggat
    11221 acgcgctggc gaatcgctaa actagaaaca ttgtttcgaa attgaacggt ggaaaggaga
    11281 ggtcatggtc tggttagcga atcccgaacg ttacgggcag atgcaatacc gctattgcgg
    11341 aaaaagcggt ttacgcctgc ccgcgttatc gctcggttta tggcacaatt tcggtcacgt
    11401 taacgcgctg gaatcacagc gtgcgatcct gcgtaaagcg tttgatttgg gcattacgca
    11461 ctttgattta gccaacaatt acgggccgcc tccaggaagc gcagaagaga actttggtcg
    11521 cctgctgcgg gaggattttg ccgcttatcg cgatgaactg attatctcta ccaaggctgg
    11581 ctacgatatg tggcccggcc cttacggctc tggcggttca cgtaaatacc tgctcgccag
    11641 cctcgaccaa agcctgaagc gtatagggct tgagtatgtc gatatctttt actctcatcg
    11701 cgtcgatgaa aatacgccga tggaagaaac cgcctctgcg ctggctcatg cggtacaaag
    11761 cggtaaggcg ctgtatgtcg ggatctcctc ttactcgcca gagcggacgc aaaaaatggt
    11821 cgagttgctg cacgagtgga aaattccgct tttaattcat caaccttcgt acaatttact
    11881 gaaccgctgg gtggataaaa gcggcctgct ggataccctg caaaataacg gcgtgggctg
    11941 tattgccttt actcctctgg ctcagggatt gctgaccgga aaatatctca acggcattcc
    12001 gcaagattca cggatgcatc gtgaagggaa taaagttcgt ggtctgacgc cgaaaatgct
    12061 taccgaagcc aacctcaaca gcctgcgctt attgaatgaa atggcacagc agcgtggaca
    12121 atcaatggcg caaatggcgt taagctggtt gctgaaagat gatcgcgtga cgtcggtatt
    12181 gattggtgcc agccgcgcgg agcaactaga ggagaacgtg caggcgctga ataatctgac
    12241 atttagcacc gaggagctgg cgcagattga tcagcatatc gccgatggcg agctgaatct
    12301 gtggcaggcg tcgtccgata aatgacctgt taataacggg cgcgcgaacc gcgcccgaat
    12361 cagatcagtg attatgacga gtcagtcggt caagatagcc catcacaaat gcagaaagca
    12421 caaacgtcag atggataatg acgtaccaca tcagtttgtt atccgggaca tttttcgcat
    12481 ccataaagac gcgcagtaag tgaatggaag aaattgccac aatcgacgct gctactttgt
    12541 ttttcagcga cgttgcgtcc attttcccca gccagttcag cttctctttg ttctcggaga
    12601 tatccagctg tgagacgaaa ttctcataac cggaaaacat caccatcacc agcaaaccgc
    12661 caaccagcgt catatccacc agcgacagca acacgaggat caaatctgat tccgccatcg
    12721 agaagatatt cggcagtacg tgaataatct cctggaagaa cttcagcgcc agggcaacta
    12781 acgccagcga aaggccaaag tacacggggg caagcagcca gcgagaagca tacattgcat
    12841 tttcaagaaa acgttccata cattcctgtc gtgtagttaa aacgggcaac agtatatcgc
    12901 aattgagtaa acagcacgca acagcaaatc aacatatacg ttaaaccttt gtcgcataat
    12961 tctgaggttt agggatcttc ctggttccct tatcaacgca tcgagagcgt ctacccttaa
    13021 aggacgttga acctgaaggg agaaaaacga tgtctcattt aaaagacccg accacgcagt
    13081 attacactgg tgaatatccc aaacagaaac aaccgacgcc aggcatccag gcgaagatga
    13141 caccggtacc ggattgcggc gagaaaacct atgttggtag cggtcgcctg aaagatcgta
    13201 aagcactggt gacagggggc gattccggaa taggtcgcgc tgccgccatc gcttacgcgc
    13261 gtgaaggggc tgacgtggcg atcagttatc ttcccgtgga agaagaagac gctcaggatg
    13321 tgaaaaagat cattgaagaa tgcggacgca aagccgttct gctgccaggc gatttaagcg
    13381 atgagaaatt cgcccgttcg ctggttcacg aagcgcacaa ggcgttaggc gggctggata
    13441 ttatggcgct ggtcgccggg aaacaggttg ccattccgga tattgcagac ctcaccagcg
    13501 aacagtttca aaagaccttt gccattaacg ttttcgcgct gttctggcta acccaggaag
    13561 cgatccccct gctaccgaaa ggtgcaagta tcatcaccac ttcgtcaatc caggcatacc
    13621 agccaagtcc gcatttactg gactatgcgg ctacgaaggc ggcgattctg aactacagcc
    13681 gtggcctggc aaaacaggtc gcggagaaag gtattcgggt gaatattgtc gcgccaggcc
    13741 ctatctggac ggcactgcaa atttccggcg gacaaacgca ggataagatc ccgcagtttg
    13801 gtcagaaaac gccgatgaag cgtgcggggc aaccggcgga actggcccct gtatatgttt
    13861 atctggcaag tcaggagtcg agctacgtca ccgcagaagt gcacggcgtg tgcggcggcg
    13921 agcatttagg ttaaaaaaat gcccggttgt gaaaagcaac cgggcatcat tgtgaattca
    13981 ctcttacctg atgtggcgaa aacgccttat ccggcctaca aacgcacgaa gattcaacat
    14041 gttgcaatag attttgtagg cctgatgagc gtagcgcatc aggcaattct actttacttc
    14101 gctttggcgg tttcttcgcc gaccagacct atcttcaggt aacccgcctg atgcagcgta
    14161 tccattacct tcatcaacgt ctcgtaatcg acggttttgt ccgctcggaa gaagatggtg
    14221 gtgtctttct tgccttcggt taacgcattc aacgccgtaa tcattgtttc atcggtgacc
    14281 ggatcgttac cgataaacat cgagttgtct gccttcaccg acagataaac cggtttttcc
    14341 gggcgcggct gcggcgtgct ggtagaagca ggcaagttca ccttcacatc taccgtcgct
    14401 aacggtgccg ccaccataaa gataatcagc agaaccaaca tcacgtcgat aaacggcgtc
    14461 acgttgatat catgcatttc gccgttatcg tcgaggtttt cgttaagatg cattgccatc
    14521 ggacattatc ctgcgcgtaa tttttgtgcg acacgaaccg gatgcgcagc ggcgctggct
    14581 tccagatcca ggtcacggct ttgcagcaac aatacctgcg ctgcaacatc acccagcatc
    14641 gctttaaagc cgccaatctg gcgtgcaaat acgttataga taacgaccgc aggaatcgct
    14701 gccacgaggc cgattgccgt tgctaacaga gcttctgcga tacccggcgc aacgactgcc
    14761 aggttagtgg tctgcgtttg cgcgatacca ataaagctgt tcatgatgcc ccagaccgta
    14821 ccaaacagac caacgaacgg agaaatcgcg ccgatggttg ccagatagcc gttaccgcgg
    14881 cccatttgac gacccactgc ggcgacccga cgctccagac ggaagctggt acgttcttta
    14941 ataccttcgt tatcgtcgct gccttctgac agttccagct cattctgcgc ttcattgagc
    15001 aaatgcaggc ttaagctttt gctaccaaaa tcagcggcga tatcgttggc ctggtttaag
    15061 gaacgcgctt cagccagcag ttgctgctcg cgcttaaggc gacgcttctg attgaagaac
    15121 tctacgctct tactgaagaa gattgcccaa gtgactacgg aggccaaaat aagcccaatc
    15181 atcacgcact taacgacaat atcggcgtgc tgatacatac cccagacgga aaggtccgtc
    15241 tgcatcaaat tattacccac tgtgtatctc caggacgcaa gtcacaaaat ctgcgcataa
    15301 taatatcaaa atgacgtcga attgatagtc gttctcatta ctatttgcat actgccgtac
    15361 ctttgctttc ttttccttgc gtttacgcag taaaaaagtc accagcacgc catttgcgaa
    15421 aattttctgc tttatgccaa ttcttcagga tgcgcccgcg aatattcatg ctagtttaga
    15481 catccagacg tataaaaaca ggaatcccga gatggcggac aaaaagcttg atactcaact
    15541 ggtgaatgca ggacgcagca aaaaatacac tctcggcgcg gtaaatagcg tgattcagcg
    15601 cgcttcttcg ctggtctttg actgtgtgga agccaaaaag cacgcgacgc gcaatcgcgc
    15661 caatggcgag ttgttctatg gacggcgcgg aacgttaacc catttctcct tacaacaagc
    15721 gatgtgtgaa ctggaaggtg gcgcaggctg cgtgctattt ccctgcgggg cggcagcggt
    15781 tgctaattcc attcttgctt ttgtcgaaca gggcgatcat gtgttgatga ccaacaccgc
    15841 ctatgaaccg agtcaggatt tctgtagcaa aatcctcagc aaactgggcg taacgacatc
    15901 gtggtttgat ccgctgattg gtgccgatat cgttaagcat ctgcagccaa acactaaaat
    15961 cgtgtttctg gaatcgccag gctccatcac catggaagtc cacgacgttc cggcgattgt
    16021 tgccgccgta cgcagtgtgg tgccggatgc catcattatg atcgacaaca cctgggcagc
    16081 cggtgtgctg tttaaggcgc tggattttgg catcgatgtt tctattcaag ccgccaccaa
    16141 atatctggtt gggcattcag atgcgatgat tggcactgcc gtgtgcaatg cccgttgctg
    16201 ggagcagcta cgggaaaacg cctatctgat gggccagatg gtcgatgccg ataccgccta
    16261 tataaccagc cgtggcctgc gcacattagg tgtgcgtttg cgtcaacatc atgaaagcag
    16321 tctgaaagtg gctgaatggc tggcagaaca tccgcaagta gcgcgagtta accaccctgc
    16381 tctgcctggc agtaaaggcc acgaattctg gaaacgagac tttacaggca gcagcgggct
    16441 attttccttt gtgcttaaga aaaaactcag taatgaagag ctggcgaact atctggataa
    16501 cttcagttta ttcagcatgg cctactcgtg gggcgggtat gaatcgttga tcctggcgaa
    16561 tcaaccagaa catatcgccg ccattcgccc acaaggcgag atcgatttta gcgggacctt
    16621 gattcgcctg catattggtc tggaagatgt cgacgatctg attgccgatc tggacgccgg
    16681 ttttgcgcga attgtataac attgccactt ttggacaatt ttgcagacat ttaattgtga
    16741 aaagtcttaa attgttgcgt ccgggatcaa ggcgtcccgg acgaatcagg agtacaatag
    16801 gcagataaag gcttaaacgc tgttccacag gaaagtccat ggctgttatt caagatatca
    16861 tcgctgcgct ctggcaacac gactttgccg cgctggcgga tcctcatatt gttagcgttg
    16921 tttactttgt catgtttgcc acgctgtttt tagaaaacgg cctgctgccc gcctcatttt
    16981 tgccaggcga cagcttgttg atactggcag gcgcattgat tgcccaaggg gttatggatt
    17041 ttctgcctac gattgcgatt ctgaccgccg cagcaagtct gggctgctgg ctaagttata
    17101 ttcaggggcg ctggttaggg aataccaaaa cggtgaaagg ctggctggca cagcttcctg
    17161 ctaaatatca ccagcgcgcc acctgcatgt ttgaccgcca cggtctgctg gcgctgctgg
    17221 ctggacgttt tcttgcattt gtccgtacgc tgctgccaac catggcggga atttccggtc
    17281 tgccaaaccg ccgcttccag tttttcaact ggttaagtgg attgctgtgg gtcagcgtgg
    17341 taaccagttt tggctatgcc ttaagtatga ttccgttcgt taaacgccat gaagatcagg
    17401 taatgacgtt cctgatgatc ctgccaattg ccttgttaac cgctggcttg ttaggcacgc
    17461 tgtttgtagt gattaaaaaa aaatactgta acgcctgacg attttccccg ttcccggctg
    17521 ctgtaccggg aacgtattta attcccctgc atcgcccgca ttcttgccgc atcttccccc
    17581 ggcgtcacac cgaagtaacg tttaaactca cggctaaatt gcgatgcgct ttcatagtcg
    17641 acgcgcatcg ctgctgcgct ggccttcatg ccgtcatgga tgatcatcat ccgcgcctta
    17701 tgcagacggt aattcttcaa atactgcaac ggcgaggtgc tggtgacaga cttaaaatta
    17761 tgatggaacg ccgatacgct catgttggct tctgccgcca gttgctcgac gctcaggttt
    17821 tcggtgtatt tattctcaat ccgtttcagc acgcggctaa tcagactgaa gtgagtctgg
    17881 cgactgacca gcgccagtaa cgcgccgccg caaggtccgg tcagcacgta gtacagaatt
    17941 tcgcggatga tctgtttgcc gagaatacgc gcatccagtg gtcgctccat cacgtcgagt
    18001 aaccgctccg ccgcgcataa aatctcttct gataacgtgg cggagttaat cccgctggct
    18061 gccatcgacg gctggaaatg ctcatcttcg ccaatgtcca tcaacagttc ctgtaactgc
    18121 aaaatatcga cattgagacg caatcctgcc agcggcacct ctgacgtggc ataggtttcg
    18181 cactcaaacg gcaacggcac cgtcagcagc aggtattcat tggcatcata acgaaacacg
    18241 cgttcattga tataaccgat tttatgcccg gaaaagagaa ttatgatgcc aggctcgtac
    18301 atcaccggtg tacgtgcgaa aggcgtctcg ccatacaaca aacgcacatc gggcaacagt
    18361 tctgacaaac tattttcttt atttttcagt ttattaactt tatccgccag caagcggcaa
    18421 atctcttcac gtttcatatc gcgtaatttc ttaggaataa tgcggcaatt tgattgtgcg
    18481 caattttgta gcatttctcc agcactctgg agaaataggc aagacattgg cagaaatgag
    18541 cattgagagc cagggcgctg gcgatcacaa tgaaaaacat caggcagatc gttctctgcc
    18601 ctcatattgg cccagcaaag ggagcaagta atgaacaact ttaatctgca caccccaacc
    18661 cgcattctgt ttggtaaagg cgcaatcgct ggtttacgcg aacaaattcc tcacgatgct
    18721 cgcgtattga ttacctacgg cggcggcagc gtgaaaaaaa tcggcgttct cgatcaagtt
    18781 ctggatgccc tgaaaggcat ggacgtgctg gaatttggcg gtattgagcc aaacccggct
    18841 tatgaaacgc tgatgaacgc cgtgaaactg gttcgcgaac agaaagtgac tttcctgctg
    18901 gcggttggcg gcggttctgt actggacggc accaaattta tcgccgcagc ggctaactat
    18961 ccggaaaata tcgatccgtg gcacattctg caaacgggcg gtaaagagat taaaagcgcc
    19021 atcccgatgg gctgtgtgct gacgctgcca gcaaccggtt cagaatccaa cgcaggcgcg
    19081 gtgatctccc gtaaaaccac aggcgacaag caggcgttcc attctgccca tgttcagccg
    19141 gtatttgccg tgctcgatcc ggtttatacc tacaccctgc cgccgcgtca ggtggctaac
    19201 gacgtagtgg acgcctttgt acacaccgtg gaacagtatg ttaccaaacc ggttgatgcc
    19261 aaaattcagg accgtttcgc agaaggcatt ttgctgacgc taatcgaaga tggtccgaaa
    19321 gccctgaaag agccagaaaa ctacgatgtg cgcgccaacg tcatgtgggc ggcgactcag
    19381 gcgctgaacg gtttgattgg cgctggcgta ccgcaggact gggcaacgca tatgctgggc
    19441 cacgaactga ctgcgatgca cggtctggat cacgcgcaaa cactggctat cgtcctgcct
    19501 gcactgtgga atgaaaaacg cgagaccaag cgcgctaagc tgctgcaata tgctgaacgc
    19561 gtctggaaca tcactgaagg ttccgacgat gagcgtattg acgccgcgat tgccgcaacc
    19621 cgcaatttct ttgagcaatt aggcgtgccg acccacctct ccgactacgg tctggacggc
    19681 agctccatcc cggctttgct gaaaaaactg gaagagcacg gcatgaccca actgggcgaa
    19741 aatcatgaca ttacgttgga tgtcagccgc cgtatatacg aagccgcccg ctaagctttt
    19801 tacgcctcaa actttcgttt tcgggcattt cgtccagact taagttcaca acacctcacc
    19861 ggagcctgct ccggtgagtt catataaagg aggaacgtat ggctaatcca accgttatta
    19921 agctacagga tggcaatgtc atgccccagc tgggactggg cgtctggcaa gcaagtaatg
    19981 aggaagtaat caccgccatt caaaaagcgt tagaagtggg ttatcgctcg attgataccg
    20041 ccgcggccta caagaacgaa gaaggtgtcg gcaaagccct gaaaaatgcc tcagtcaaca
    20101 gagaagaact gttcatcacc actaagctgt ggaacgacga ccacaagcgc ccccgcgaag
    20161 ccctgctcga cagcctgaaa aaactccagc ttgattatat cgacctctac ttaatgcact
    20221 ggcccgttcc cgctatcgac cattatgtcg aagcatggaa aggcatgatc gaattgcaaa
    20281 aagagggatt aatcaaaagc atcggcgtgt gcaacttcca gatccatcac ctgcaacgcc
    20341 tgattgatga aactggcgtg acgcctgtga taaaccagat cgaacttcat ccgctgatgc
    20401 aacaacgcca gctacacgcc tggaacgcga cacacaaaat ccagaccgaa tcctggagcc
    20461 cattagcgca aggagggaaa ggcgttttcg atcagaaagt cattcgcgat ctggcagata
    20521 aatacggcaa agccccggcg cagattgtta tccgctggca tctggatagc ggcctggtgg
    20581 tgatcccgaa atcggtcaca ccttcacgta ttgccgaaaa ctttgatgtc tgggatttcc
    20641 gtctcgacaa agacgaactc ggcgaaattg caaaactcga tcagggcaag cgtctcggtc
    20701 ccgatcctga ccagttcggc ggctaacatg caaattctcc cggtgacggt aatgttccgc
    20761 taccggactt ttcagaaatc atttattccc ctcgcgtccc gcccgttgtt actcttcctt
    20821 gttcaggaat gccaaatata aggacatcat catgcagagc cggaagctct taaaagaaca
    20881 actcatctat atccgggata aacgcaacgg agaggtgaaa aacagatgaa aataatactt
    20941 ctgtttttag cagccctggc aagttttacc gtacacgcgc agcctccttc acagaccgta
    21001 gaacaaacag tccggcagat ttatcagaac tataaatcag atgccagtac accttatttc
    21061 ggtgaaaccg gagagcgggc gataacttca gcgcgtattc aacaggcgct taccctgaac
    21121 gacaatctta cgctgccggg caatattggc tggctggatt atgatccggt ttgtgattgt
    21181 caggattttg gcgatctggt gctagaaagc gttgcgataa cccaaactga cgccgatcat
    21241 gccgatgccg ttgtgcgctt tcgtatcttt aaagatgata aagaaaagac cacgcagaca
    21301 ctgaaaatgg tggcggaaaa tggtcgttgg gtcattgacg atattgtcag caatcatggc
    21361 agcgtcttac aagcagttaa tagcgagaat gaaaaaacgc tggccgcttt agcttcgttg
    21421 caaaaagaac agccggaagc ctttgttgcc gaactctttg aacatattgc tgattatagc
    21481 tggccgtgga cgtgggtggt ttccgactct taccgccagg cggttaatgc cttctataaa
    21541 accaccttca agacggccaa taatcccgat gaagatatgc aaatagaacg gcaatttatt
    21601 tacgacaatc cgatctgttt tggcgaagag tcgctatttt cacgcgttga tgaaattcga
    21661 gtcctggaga aaaccgccga ttccgcccgc attcatgttc gttttacgct gaccaatggc
    21721 aacaacgaag agcaagaact ggttttacag cggcgcgaag gcaagtggga aatcgctgat
    21781 tttatccgcc cgaacagcgg cagcctactt aagcagattg aggcaaaaac cgccgccaga
    21841 ttaaagcaat gagctgaatt aaataacaat tagccggaac aataaataaa agggaacact
    21901 atatgaaaac gattttcacc gtgggagctg ttgttctggc aacctgcttg ctcagtggct
    21961 gcgtcaatga gcaaaaggtc aatcaactgg cgagcaatgt acaaacatta aatgccaaaa
    22021 tcgcccggct tgagcaggat ataaaagcac tacgcccaca aatctatgct gccaaatccg
    22081 aagctaacag agccaatacg cgtcttgatg ctcaggacta ttttgattgc ctgcgctgct
    22141 tgcgtatgta cgcagaatga taaaaaaatc cccggcagca tgtcagttgc cggggatttt
    22201 ttttaacgtc caaccgccgc tttagggcgt ttcttcgcac cagcattcac cggacgagat
    22261 tgcgtagacg acgctttttt tgccgtagca ggcgtctgac gctgggtcgc catcggcgta
    22321 tgtttagtca acgccggacg ggtattgcgg ttctggcgac gagcttcacg catctcttca
    22381 atggttggcg caggcactaa gcaatcgcga cggctgccaa tcagatgctt tttgcccatc
    22441 gcttccagcg ccgggcggat taacggccag tttgccggat cgtggtaacg caacaacgct
    22501 ttatgcaaac gacgctgttt gtcgcccttc ggtacgaaga cgtcttcgct cttataacca
    22561 atcttcgcca gcggtttttt cccggtgtaa tacatggtgg ttgagttcgc cagcggcgat
    22621 gggtagaagt tctgtacctg gtcgagacgg aaacgatgct ttttcagcca cagcgccaaa
    22681 ttcaccatat cttcatcacg cgtaccgggg tgcgcagaga tgaaatacgg gatcagatac
    22741 tgctctttac ctgcctgttt cgagtaggta tcgaacagct ctttaaagcg gtcatagctg
    22801 cccatgcccg gcttcatcat cttcgacaac ggcccttctt cggtatgttc cggagcaatc
    22861 ttcagataac cgccgacgtg atgggtcgcc aactctttga tatagcgcgg atcttccacg
    22921 gcgatgtcat aacgtacgcc tgaggcgatg aggatttttt taatgccttt cagatcacgc
    22981 gcacggcgat agaggttgat cgtcggttcg tggttagtgt ccatgtgcgg acaaatatcc
    23041 ggataaacgc acgacaaacg gcgacaagtt tgttcagcgc gtggcgattt gcagtgcaac
    23101 atatacatgt tggcagttgg cccaccgaga tcggaaatca cgcccgtaaa acctggaacg
    23161 gtgtcgcgga tcgcttcgat ctcattaatg atcgaatctt ccgaacggct ctgaataatg
    23221 cgcccttcgt gctcggtaat agaacagaaa gaacagccgc caaagcagcc gcgcataatg
    23281 ttgaccgaaa aacggatcat ttcgtaagcc ggaatacggg cattgccata ggccggatgt
    23341 ggcacgcgct tgtacggcag cgcaaaaacg ctgtccatct cttcggtaga aagcgggata
    23401 gcaggcgggt tgatccacac atagcggtca ccgtgttttt gcatcaatgc gcggacgcag
    23461 cctgggttgg tttcgtggtg cagaatacgc gaagcatggg cgtacagcac tttatcgccc
    23521 ttcactttct cgaaagaagg cagcaacacg taggtttttt cccacggttt cgggcgcggt
    23581 ggctgcacgg ttacggcttt ggcttcctgc tttttcggtg ccaccggttt gttatccgcg
    23641 cacggcaaat cttcaccata cggatgcggg attgggtcga tttttccagg ggtatcaaga
    23701 cggggggaat ccacgccgct ccagccaggc agcgcctctt tcacgataat cgcggtatta
    23761 cgcacatcgc ggatttcact aatcggctcg cccatcgcca gacggtgcgc cacctccacc
    23821 agcggacgct caccgttacc aaacatcagc atgtcggctt tcgaatccac cagcacggaa
    23881 cggcgcacgg tatcggacca gtaatcataa tgtgcggtac ggcgcagact agcctcaata
    23941 ccgccgagga tcaccggtac atctttccac gcctctttac aacgctgggt ataaaccagt
    24001 gtggcgcgat ccgggcgctt acccgcgacg ttatccggcg tgtaggcatc gtcatgacgt
    24061 aaacggcgat cggcggtata acggttgatc atcgaatcca tgttgccagc agtaacaccg
    24121 aaaaacagat tcggtttacc cagacgcata aagtcgtctt tgctgctcca gtccggctgg
    24181 gcgatgatcc cgacgcgaaa gccctgtgct tccagcatac gaccgcaaat cgccatcccg
    24241 aagcttgggt gatcgacata cgcgtcgcca gtaaccaaaa tgatgtcgca gctatcccag
    24301 ccaagttgat ccatctcttc acgagacatc ggcaaaaacg gtgccggtcc aaaacaggcg
    24361 gcccagtact gcggccagga gaacaggtcg cgatccggtt ggatcaggga gatagagctc
    24421 attttgcttc cagaaatgat aaaaaaataa tcaaaggccg gggattataa gccggaacga
    24481 aagagaaatc gaaaagtatt ccatactcgc cctcctcggg cgagtatgaa gattacggta
    24541 ccggattgac taacagttgc ccaatcgagc cacggtccgc catttccagc gtctgactgt
    24601 tgaagtagaa cgggaagtgc gcccaggaag gctgaccgaa ataaacaagc agctccactt
    24661 gtccgtcaac ccaaacggta tctttccagc ctctgtcttc cgggaacggc atcgcaccat
    24721 tcacgttacg gatctggaac atcacgcctt caatatggaa cgcttgcggc tcgtccgcgc
    24781 gtaccgtcca gcgttcccac gttccttgct gcgcggtgac atcaatacgg ttgacgtccc
    24841 acagctgtcc attaataccc gggtcatcac ccagactgat atcgcgactg cgaatcggcg
    24901 aaccggccat gatttcagtt ggcagcaagc gcatcggaag actgtctgtg accagcggca
    24961 gaaggccggt tgggcgcagc gttagcacca gggtagaaac cagaatactg gatggctcaa
    25021 agaagccgcg aatacgatca acaatgctcg ccgcttcgcc acaggtgatc gacacttcat
    25081 cgccgttgct catatccacc agaatctcgc ggcgctcgcc cggtgccagc gaaagttgct
    25141 tcaccgatac aggagcaggc aggaatccct gatcgccaga aatcacatgt aacgggcgac
    25201 catcgctcat ctgtagttga tagcgacgag agttcgacgc gttcagcagt cgcaagcgca
    25261 cccagccacg cgagacttca acgtacgggc tttgtacacc gttaaccagc agcgtatcac
    25321 caacaaagcc gccgcttccc ggttcgttgt attctggcgt accaaagtta tccagccgtt
    25381 tatcctggat aatgaccgga aaatcatcca catcataatg gttggggata ggcagcgact
    25441 tgctgacttc atcttccacc agccacattc cggcaaggcc gttatagacc tgctgagccg
    25501 tgcggttggg agtattggcg tgataccaca gagtagctgc gttctggcga ataggcagta
    25561 cgggtgccca gtcagcgttt ggcgacatca tccgtgccgg accgcccatc agcgggccag
    25621 gtacctgtag cccggccacc gtcattgaga cattttctgt caggcggttg ctgtaaataa
    25681 gcttaacatc gtcgcccttc cagacgcgga tagtcggccc caggtaacga ccattgattc
    25741 cccagaccga cgcgcgtgtc cctggcgtaa atgaccagtg cgcacgttgt acagtcataa
    25801 acagcggttg cccacggcga gattcaagta gcggcggaac gggtagcggt tgctgttgcc
    25861 cggctgcgct ggccttcagg ggaacagcgc ctgcacaaag tgcaatcccc gatgcctgaa
    25921 tgaactgacg ccgactgagt gacatatttg ctccatgtaa aactggctaa agtaataccg
    25981 tgcaggaaag tgttccccgc aaaaatacag attgccgact taaacttttc cggcggcttc
    26041 gcgttctgcg acttctttat cgagctcggc gattttttgt tccattatcg aacgacaatg
    26101 ggcagccagc tcacgaacct gatctttgcc atactgactg acgtcaattg gcggcagcat
    26161 ttcgacaatc accagaccgt tgtgcagtcg attaagatta atcttattcg aagttgtaga
    26221 gacgcacacg ggaataatcg ggacgcccgc cgcaattgcc gcgtgaaatg ctccagtctt
    26281 gaacggtagc aggccgcgac cacggctgcg ggttccttcc gggaacatcc agatggaaat
    26341 acggcgtttt ttgaaatgat tcacgacttc cgcaatggtg ccgtgagctt tagtgcgatt
    26401 gtttctgtcg atcaataagt tgccggttaa ccagtacaac tgcccgaaga aggggatcca
    26461 cagcaagctc tttttaccta ccgtcaccgt cggcggttgc acaatgttcg atgctgtcac
    26521 catgtcatag ttgttctggt ggttagcgat atagatagca ttgccgtagc tttcagcatc
    26581 tgcaggtttg cggcactcaa ctttcaggcc gaacagcggc gcaagacggc caaacatatg
    26641 cccaaaggtg gccacatgtt tcgggttacg cgggctgaaa aggcagtaaa tggagccgaa
    26701 tacacagact aagatgctgt aaatcacggt aataataaga cgaaagatat atagcataac
    26761 accctctgaa ggtctgagag actggcattg tacgtcacct gaaatcaggt tagggcattg
    26821 ttaaaagccg ctctctggaa aagcgtattg ttaatcgcaa cgcatgaata aacaacggtt
    26881 tcaagggaat ttttatcgtt tccgggggtt gaatacgctg ccggatgacg acttaacgtt
    26941 tcatccggcg ttccttgcaa gcgggaggaa acaggccctc cccggcatat tactcttcgc
    27001 tatcaccgtt gctggcacgg cgaggagagt cgatctcaac acgatcgata cgctgcaaac
    27061 cgcgcatcaa cgtaccgcgg cgtccacgtt caccagtgac tttctgtaac tcttccgggc
    27121 gcagtttaat tttgcgtttc ccaacatgaa tggtcagcgt gctttgcggc ggcagaacgt
    27181 acaattgcgc cagaccatct tctccacgcg cggcttctgc cgatggaatg ttgataatct
    27241 tgttgccttt gcccttcgac agctgcggca gatcgcttac cgggaacatc aacatacggc
    27301 ctgcctgagt gattgccagc agcatatcgg aagcatcttc aatcaccacc ggcggcataa
    27361 catgggcatt ttccggtaag gtgatcaaag ccttacctgc acggttacgc gccaccagat
    27421 cgttaaaggt gcagacgaaa ccgtaacccg catcggaagc catcagcagt ttctgatcgt
    27481 cgctttccat cagcatatgg tcaacggtcg ccccaggcgg caacgttaat ttgccggtga
    27541 gtggttcgcc ctgaccacgc gccgacggca gcgtaatcgg gtcgatggca tagctacgac
    27601 cggtggaatc aacaaacact accgattggt tgctcttacc tttcaccgcc actttgaagc
    27661 tatcacccgc tttataattc aggcccggcg cgtcgatatc atggccttta gcgctgcgta
    27721 cccagcccat ctgcgacagc acaatggtga caggttcaga cggcagcatg tcgtgctcgc
    27781 tcatcgcttt cgcttcttcg cgttcctgca acggcgaacg acgatcgtca ccgtaggctt
    27841 gcgcgtctgc ctgcagttct ttcttcagca ggttattcat tttacgctcg gaagccaaaa
    27901 tgccctgcaa ctggtcgcgc tctttttcca gctcactctg ctcaccgcga atcttcatct
    27961 cttccagttt ggcaagatga cgcagtttca gttcgaggat cgcttccgcc tgggtttccg
    28021 taaggccaaa ccgcgacatc agcgccggtt tcggttcatc ttcgttacga atgatctcaa
    28081 tcacttcgtc gatgttgaga aacgccacca gcaaaccttc caggatatgc aggcgcttga
    28141 ggactttctc cagacgatag ttcagtcggc ggcgcacggt atcgcgacgg aacaccagcc
    28201 attcggagag gatttccagc aggtttttca ccgccggacg accatccaga ccgatcatat
    28261 tgaggttaat gcgatagctc ttttccagat cggtggtagc gaagaggtgg ttcatcacct
    28321 gatccatatc cacgcggttg gaacgcggca caatcaccag acgggtcggg ttctcgtggt
    28381 cagattcatc gcgcagatcg tcaaccatcg gcagcttttt gttgcgcatt tgcgcagcaa
    28441 tttgctccag tacacgcgca cctgaaacct gatgcggtaa tgcgctgata accaccgcgc
    28501 catcttcttt cttccacacc gcgcgcatac gtactgaacc acgtccgttc tcgtagattt
    28561 tacggatctc ggcgcgcgaa gtgataattt ccgcttcagt cggataatcc ggcccctgca
    28621 cgatatccag cagctgatcg agcgtggttt tcggctggtc gattaatgcg attgccgcct
    28681 gagctacttc acgcaggtta tgcggtggaa tatcggtcgc catgccgacg gcaataccgg
    28741 tggtgccgtt aagcaaaatg tttggcagac gagcaggcag cattttcggc tcctgcaaag
    28801 tgccgtcgaa gtttggcacc cagtcagccg tcccctgccc cagctcgctc aatagcagct
    28861 cggaatattt cgacaaccgg gattcggtgt aacgcattgc cgcgaacgat ttcggatcgt
    28921 ccggcgcgcc ccagttcccc tgaccatcaa ccagcggata acggtaagag aacggctgcg
    28981 ccatcaggac catcgcttca taacaggcgc tatcgccgtg cggatggtat ttacccagta
    29041 cgtcaccgac ggtacgggcc gattttttaa atttggcgct ggcattcagg cccagttcag
    29101 acatcgcata cacaatgcgg cgctgaacag gtttcagacc atcaccaata aacggcaacg
    29161 cacggtccat gatcacgtac atggagtagt ttaagtaggc gttttccgta aattcatgta
    29221 gcgcaaggcg ctctgccata tcgctcatta attctgattc ctcaactgat tcgccagcct
    29281 tcaaaggggc aatattgccg gagatactac attatctgcc gcgtcgagtc acaggagatc
    29341 agcgtgaata gcgccgccgg acgtgctccg acggcgataa aggcatcaat gtgccttgat
    29401 atacaacgct ttgctgtaga cttgatcggc atagagggca gcacaaggtt gcgcccatgc
    29461 cacaccactc ggcatggggg tccgctccgg gcgattcgag aagttgaggt tatgcgagtc
    29521 cagggaagcc taaagcatca aagtagtcga ctataagtat atggttcagc ccagtttagc
    29581 tgtgtctcca ccagtgcccc gcgcctcctg ccataagtgc ggcttgctca tgggtacccc
    29641 cgagacggct tacggcgcac cggataataa atttcgcgta tacatggtat tccttaccgg
    29701 agaaattata agcccaacaa caatacgcct gtggcattgt tcgctcaaac ttatcgcgag
    29761 tgatttggct cacactccgg taaagattcc ccatcacgct aacggcacat aaacatccgt
    29821 ttccagtcgc tgctccgtca ccccttcggc aagattggtg tagtgaaaca gaatcggtgc
    29881 tttacgcatt ttctcgccgc ttgcaggcag ccagtggcga ataatgcccc atatcgtgtg
    29941 tgaaatatcg tccagctcgc caacgtggcg ggccacggca taacgtccac cggtaagttc
    30001 accattgctc acaccataac gattatcggg aatcggttcg ctaacgctgc cgcagatatc
    30061 aaagcgaaac gcttccggtg cggtggtggc agggtcgtcc caggcgacgc caaaagtctg
    30121 gctttggtta acaggagaaa gccccgtctc tttacgccac atgataaatt tcgccacact
    30181 gtagttgagc aactcagggc tacagcggtg cggcaacatc gccacaggga ttgatgggaa
    30241 atcgataatg tttacatcca gtgtcaggtt tgtcattttg cctccctgcg aaattattcc
    30301 cgggaacgcg tcccgggagc ggtaacaatt acggatttac tttgcggatc tgtttgacgt
    30361 caatttcaac agaattccag tctttatcga cttcaccctg aatctcaacc gtatctttcg
    30421 gcgtcaccgt cacaccgttc cagcgtttgt ggtcgatatc aacattgata gtaccgctgg
    30481 catctttaaa cacgtagaga tcgtcagaga tgcgttcaac gatattgccg cgcagggtta
    30541 cccaggtgtc gtcacgcagg gattttgcgc tttctacagt cgttacgctg ccgttcggcc
    30601 cctggaatcc tccggcctga ctttgcgttg ccgatgggcc agaaaaaccg ccctgctctg
    30661 ctgccatcac cggtgcactg cacagggcca ttactgcgat tactgctgcg aattttttca
    30721 tgtttattac tccctttaat gtctgtttcc gagcatttaa caagatagtc cttaacaact
    30781 tcttaaggga aaaaaataaa atttaatgct gtacagagcg cgttacaaca cggtttactg
    30841 gcagcaaata cggttatcgc agggatgaaa aaatgcgaat tttactgata gaagatgaca
    30901 tgctgattgg cgacggcatc aaaactggcc ttagtaaaat gggttttagt gtcgactggt
    30961 ttacacaagg tcgtcaggga aaagaggcgc tttatagcgc accttatgat gcggtgatcc
    31021 tggatttaac cttaccaggc atggatggtc gcgatatttt gcgcgaatgg cgagagaaag
    31081 gtcagcgtga gccggtactg atcctgaccg cgcgtgatgc gttagcggaa cgtgtagaag
    31141 ggctgcgtct gggagctgac gattatctgt gtaaaccttt tgcgttgata gaagtcgccg
    31201 ccaggctgga ggctctgatg cgccgaacca acggccaggc cagcaacgag ctgcgccacg
    31261 gtaacgttat gctcgacccc ggcaaacgta tcgccacgct ggctggcgaa cccttaacac
    31321 tgaaaccaaa agaatttgcc ctgctggaat tactgatgcg taacgctggt cgggtactgc
    31381 cgcgcaaact gattgaagag aaactgtata cctgggacga agaggtcacc agtaatgccg
    31441 ttgaagtgca tgtgcatcat ctgcgacgca aactcggtag tgattttatt cgtaccgtgc
    31501 atggtattgg ctacacatta ggtgagaaat gaaatttacc caacgtctta gtctgcgcgt
    31561 caggctgacg ctaatctttt taattctggc ctcggtgacc tggctgcttt ccagctttgt
    31621 cgcctggaaa caaacaacgg ataacgtcga tgaattgttc gacacccaac tgatgctgtt
    31681 tgccaagcgg ttaagtacgc tcgatctcaa cgaaatcaac gcggcggatc gcatggcaca
    31741 gacgccaaat aaattaaaac acggtcatgt tgatgacgat gcgctgacct ttgccatctt
    31801 tacccacgac ggcagaatgg tccttaatga tggcgataac ggagaagata ttccctatag
    31861 ctatcaacgg gaaggttttg ctgacgggca actggtcggt gaagacgatc cttggcgttt
    31921 tgtctggatg acctcacctg atggcaaata tcgcatcgtt gttggccagg aatgggaata
    31981 ccgtgaagac atggcgctgg cgattgttgc cgggcaattg atcccgtggc tggtcgcact
    32041 gccgattatg ttaatcatca tgatggtact actgggtcgt gaactcgcgc cgctgaacaa
    32101 actggcgctg gcactacgta tgcgtgaccc tgactcggaa aaaccactaa acgcgactgg
    32161 cgtacccagc gaagtgcgac cactggttga gtcgctaaat caactgttcg cccgcacaca
    32221 tgcgatgatg gttcgagaac gacgctttac ctccgatgca gctcacgaac tacgtagccc
    32281 gttaacagcg ctgaaagtgc aaaccgaagt tgcgcagctc tctgacgatg atccgcaggc
    32341 gcggaaaaaa gcactgctcc aattacattc cgggatcgat cgcgctactc gtctggttga
    32401 tcaactgctc acgctatcgc ggctggactc actggataac cttcaggacg tcgcggagat
    32461 cccgcttgaa gatctcctgc aatcgtcggt gatggatatt taccacacgg cgcagcaggc
    32521 gaaaattgac gtgcgactga cactcaatgc ccacagcatc aaacgcactg gacaaccgct
    32581 attgctaagt ttgttggtgc gaaaccttct ggataacgca gtgcgctaca gtccacaggg
    32641 cagcgtggtg gacgtcacgc tgaatgccga taatttcatc gtgagggata acggccccgg
    32701 tgtgacacca gaggcactgg cgcgaattgg cgaacgcttc tatcgccccc ccggacaaac
    32761 cgctaccggc agtggacttg ggctatcgat tgtccagcga atcgccaaat tgcatggcat
    32821 gaatgttgaa tttgggaatg cggaacaagg tggatttgag gcgaaggtga gctggtaagg
    32881 gtaagacttt tgctaaattc gcaaaagact ttgcacattt tgctaatttt accgtaccgc
    32941 tctgtgacgt actatagtcg acaaacgtct caccttgagg ttaaaaaatg agcaacaccc
    33001 tgattatcaa cggcgcgaaa aaattcgccc actccaatgg tcaactgaac gacaccctga
    33061 ccgaagtcgc ggatggcaca ctgcgcgacc tcgggcatga tgttcgcatc gttcgcgccg
    33121 acagcgacta cgatgtcaaa gcggaagtac aaaactttct ctgggctgat gtggtgatct
    33181 ggcagatgcc aggctggtgg atgggcgcgc cgtggacagt gaaaaaatac attgatgatg
    33241 tattcaccga aggtcacggg acgctgtatg ccagcgatgg ccgtacccgc aaagatccgt
    33301 caaaaaaata cggttccggc ggcctggtac agggcaaaaa atatatgctt tctctgacct
    33361 ggaacgcacc aatggaagcc ttcaccgaaa aagatcaatt cttccacggc gttggcgtcg
    33421 acggtgtgta tctgccgttc cataaagcaa accaattcct tggcatggaa ccgctgccga
    33481 catttatcgc taatgacgtg ataaaaatgc ctgatgttcc ccgctatact gaagaatatc
    33541 gcaagcatct tgtggaaatt tttggttaac tagagctcag gctttagaag gagttaacca
    33601 tgcttaccgt aatcgcagaa atccgtactc gtcctggtca acatcaccgt caggcggtat
    33661 tggatcagtt tgctaaaatc gttccaaccg tactgaaaga agaaggttgc cacggctatg
    33721 cgccaatggt ggattgcgct gctggcgtga gtttccagtc tatggcaccg gattctatcg
    33781 tgatgattga gcagtgggaa agcatcgcgc accttgaagc gcatctgcaa accccgcaca
    33841 tgaaggcgta cagcgaagcc gtaaaaggcg acgtgctgga gatgaatatt cgtattctgc
    33901 agccagggat ttaatcctgc cttgtttgcc cggccatcct gaccgggcaa tgttctttcc
    33961 tttaaacctc aatctccgcc atgtcgcctt tctcttgcaa ccagttgcgg cgatcttccg
    34021 agcgtttctt cgccagcagc atatccatca tcgcgtcagt acgctgatcg tcttcatcat
    34081 cgatagtcaa ctgcaccaga cggcgagtgt tcggatcaag cgtggtttca cgcaattgca
    34141 tcgggttcat ttcccccaga cctttaaaac gctggacgtt cggcttgcct ttcttgcgtt
    34201 ttaattgctc aagtacgccc tctttctctt cttccgtcag cgcgtaataa acctctttcc
    34261 cgagatcaat acggtagagc ggtggcagtg cgacgtaaac gtgaccgtgt ttcaccaacg
    34321 cgcggaagtg ttttacgaac aaagcgcaga gcagcgtggc aatgtgcaga ccatcagagt
    34381 ccgcatccgc gaggatacag attttgccat aacgaagctg gctcagatcg tcgctgttag
    34441 gatcgatacc gatcgctacc gaaatatcat gcacttcctg cgaggccagc acttcgtcgg
    34501 aagaaacttc ccaggtgtta aggatcttac ctttcagcgg catgatcgcc tgatattcac
    34561 gatcgcgcgc ctgcttggca gatccgcctg cggagtcacc ttccacgagg aacagttcgg
    34621 tacggttaag gtcctgcgcg gtacaatcag ccagtttgcc aggcagcgcc gggccgctgg
    34681 tcagcttttt acgcaccact tttttagccg cacgcatacg acgctgggcg ctggaaatcg
    34741 ccatctctgc cagcaactcc gccgcctgaa cgttctggtt cagccacagg ataaaggcat
    34801 ctttcaccac gccagaaacg aatgccgcgc attgacgcga agagagacgc tctttcgtct
    34861 gcccggcaaa ctgcggatcc tgcattttca ccgacagaac ataggcgcag cgatcccaga
    34921 tatcttccgc cgacagcttc acgccgcgcg gcagaatgtt gcgatattca cagaactcac
    34981 gcatcgcgtc caacaggccc tgacgcaggc cattaacatg ggtaccgccc tgcatcgttg
    35041 ggatcaggtt gacgtagctt tcggtcagca gttcaccgcc ttccggcagc cacagtagcg
    35101 cccagtccac agcttcagta tcgccagcga aattaccgat aaacggtttt tctggcagcg
    35161 tcggtaaacc gtttaccgct tccgccaggt aatcattcag accgtcctga tagcaccagc
    35221 gctgttcggt gttgttgatc tcatctttaa aggtaatctc aacgccgggg cacagtaccg
    35281 ctttggcttt cagcacatgc gtcaggcgtg aaacagaaaa tcgcgggctg tcaaagaagg
    35341 tttcatccgg ccagaagtgc acgctggtac cggtattgcg tttaccgcaa gtgccgacaa
    35401 cctgtaaatc ctgcaccttt tcgccatttt caaaggcgat gttataaacc tgaccatcgc
    35461 ggcgcacgtt aacttcaacg cgcttcgaca gggcgttaac caccgaaatc cccacgccat
    35521 gcaggccgcc agagaactgg taatttttgt tagagaattt accgcctgca tgcagacggc
    35581 aaagaatcaa ttcaaccgcc gggacaccct cttccgggtg aatatccacc ggcatcccgc
    35641 gcccatcgtc aataacttct aacgactggt cagcatgtaa gattacatcc acgcgttttg
    35701 cgtgacccgc cagtgcttca tcgacactgt tatcaataac ttcttgcccc aaatggttag
    35761 ggcgagtggt atcggtatac atccccggac ggcggcgaac cggctcaagc ccggtgagta
    35821 cctcaatggc atcagcgtta taagtttgcg tcatggttta agttagcaat tcgagttgat
    35881 cgtcagagat ggtgcagacc aagaaaatcg acgatcgggt tgaaataatc ttcgaagccc
    35941 gtgaatgcgt ggttgccgcc ttctatgaca gtctggcggc aggaagcgta gtacgccacc
    36001 gcctggcggt aatccagcac ttcatctccc gtctgttgca gcagccagat caaatccggc
    36061 gcttccagcg ggtcaatctg catgactttg agatcgtaaa tatggcgtga ctctagcaca
    36121 tattgctgcc cggtgtatgg gttctcgttc tgaccgagat agtccgtcag cagttcaaac
    36181 gggcgcaccg ccgggtttac caccactgcg ggcagcataa aacattgtga caaccaggtg
    36241 gcgtaatatc cccccagtga cgaaccgaca atacccagcg aatcgccgcc atgttccagg
    36301 acaatggatt ccagcagttc tgccgcgtcg gaaggatacg gcggcaactg cggaatgatc
    36361 atctcaacgt cagggtgatg ttccgccagc cagtttttta acaagctcgc ttttgcagag
    36421 cgcggcgagc tgttgaaacc gtgtaaataa agaagcgtag acatcagtag ccttctgaag
    36481 cggtatcagg ttggaaacgt gtgtccgcca ggcgatgcac ctcggtggtc agcgtgccat
    36541 cagcatgtaa ctcgagagta cgccagccgg gcgcgatagt atccagcgta aagttggaac
    36601 agtgcggctt aaactgcaca caggtcgacg gcgttgccag caggcggcga ccgttccagt
    36661 cgagatccag ctcctgatga atatgaccgc acagcaagta tttgacgtgc ggaaacttcg
    36721 ccagcacggt atccagttcg cccgcgttac gcagactgtg ttgatcgagc caactacaac
    36781 ccgcaggtag cggatgatga tgcagcagca gcaacgtatg gcgttctggc gcatcggcca
    36841 gtttacgttc cagccactca agctgaaact cgctcagctc accgtgcggc acgccaaaca
    36901 cctggctatc cagcaacagg atttgccatt gctcaccaat aaacacccgc ttcgccgggg
    36961 agatacccgc atcctgtaac gcgctgtaca tcgcgggctg gaaatcgtgg ttgcccggca
    37021 gccagacgca gggcgcacga aaacttgcga tgccttcagc gaaatgctga taggccgcag
    37081 aggattgatc ctgcgctaaa tcacctgtcg cgacaatcag gtcgaattcg tgctgatgtg
    37141 gacgaatcgc ctccagcacc gcctggtagc tttcccaggt gtttacccct aacagagctt
    37201 cgtgcttttg tgcaaacagg tgagtgtcgg taatttgtaa aatcctgact ctggcctcac
    37261 cagccagagg aagggttaac aggctttcca aatggtgtcc ttaggtttca cgatgctagt
    37321 aaaccggaat cgccatcgct ccatgtgcta aacagtatcg caaccagtcc gctaaaaact
    37381 gattaatttg atgcttttcg tcgcgttgat gcaacttttt attaggataa tcataccgcg
    37441 ctttgaagcg aaaaatctgc tggcttgaac acacttcagc caccatcgcg tcatgataca
    37501 gacgcaccgt cattgacgga aggctccagt aactgatcgc gggcgcagtc tgttctattg
    37561 tcaccagggt agtgtatcgg gtcgattcca caatcgtcag ccgatattgt gcgtttgcca
    37621 cctgatagct tacagtttcg ccgggtgcgt cattgcgcgg taacaaacgg cgcaattgtg
    37681 aaaagttcat ctcgcacagg cgcatcattt caggaaagtc aggtgtgtaa cgcttcattt
    37741 atgcccactc attttttaac gcttgatgat gcagctgcag ccattgcaaa gcgatgaccg
    37801 acgctgcgtt gtcgattttc ccctcttcta cccactggta tgcctgttcc cggcttacca
    37861 catgaacgcg aatatcttcg ttttcatcag ccagaccatg aataccgctt gcggtcgtgg
    37921 cgtccacttc gcccaccata attgacgaac gctcactggt gccccccggg cttgccagga
    37981 aacttaacac cggtttggtc cgtttgacta tcagtcccgc ctcttcaatc gcttcgcgac
    38041 gggcaacatc ttccacactt tcaccctctt ctatcatccc ggcaaccatc tccagtagcc
    38101 agggggtttc actggtgtcg tatgcggcaa tccgaatctg ctcaatcagc acaacttcat
    38161 cgcgcactgg gtcaaagggt agcaagactg cggcgtgacc gcgctcaaaa atttcccgcc
    38221 gtacctcatg actcatttgc ccgttgaata gacgatgacg aaatctataa agatctaatg
    38281 aaaaaaagcc gcgataaagt gtttctcgtg caataatttc tacatcgttt ttgccaaatg
    38341 taacgggcag gttgtctggc ttaagcattg ttaatgtcct ggcactaata gtgaattaaa
    38401 tgtgaatttc agcgacgttt gactgccgtt tgagcagtca tgtgttaaat tgaggcacat
    38461 taacgcccta tggcacgtaa cgccaacctt ttgcggtagc ggcttctgct agaatccgca
    38521 ataattttac agtttgatcg cgctaaatac tgcttcacca caaggaatgc aaatgaagaa
    38581 attgctcccc attcttatcg gcctgagcct ttctgggttc agttcgttga gccaggccga
    38641 gaacctgatg caagtttatc agcaagcacg ccttagtaac ccggaattgc gtaagtctgc
    38701 cgccgatcgt gatgctgcct ttgaaaaaat taatgaagcg cgcagtccat tactgccaca
    38761 gctaggttta ggtgcagatt acacctatag caacggctac cgcgacgcga acggcatcaa
    38821 ctctaacgcg accagtgcgt ccctgcagtt aactcaatcc atttttgata tgtcgaaatg
    38881 gcgtgcgtta acgctgcagg aaaaagcagc agggattcag gacgtcacat atcagaccga
    38941 tcagcaaacc ttgatcctca acaccgcgac cgcttatttc aacgtgttga atgctattga
    39001 cgttctttcc tatacacagg cacaaaaaga agcgatctac cgtcaattag atcaaaccac
    39061 ccaacgtttt aacgtgggcc tggtagcgat caccgacgtg cagaacgccc gcgcgcagta
    39121 cgataccgtg ctggcgaacg aagtgaccgc acgtaataac cttgataacg cggtagagca
    39181 gctgcgccag atcaccggta actactatcc ggaactggcg gcgctgaatg tcgaaaactt
    39241 taaaaccgac aaaccacagc cggttaacgc gctgctgaaa gaagccgaaa aacgcaacct
    39301 gtcgctgtta caggcacgct tgagccagga cctggcgcgc gagcaaattc gccaggcgca
    39361 ggatggtcac ttaccgacgc tggatttaac ggcttctacc gggatttctg acacctctta
    39421 tagcggttcg aaaacccgtg gtgccgctgg tacccagtat gacgacagca atatgggcca
    39481 gaacaaagtg ggcctgagct tctcgctgcc gatttatcag ggcggaatgg ttaactcgca
    39541 ggtgaaacag gcccagtaca actttgttgg tgccagcgag caactggaaa gcgcgcatcg
    39601 tagcgtcgtg caaaccgtac gttcctcctt caacaacatt aatgcatcta tcagtagcat
    39661 taacgcctac aaacaagccg tagtttccgc tcaaagctca ttagacgcga tggaagcggg
    39721 ctactcggtc ggtacgcgta ccattgttga tgtgttggat gcaaccacca cgctgtacaa
    39781 cgctaagcaa gagctggcaa atgcgcgtta taactacctg attaaccagt tgaatattaa
    39841 gtcagctctg ggtacgttga acgagcagga tctgctggca ctgaacaatg cgctgagcaa
    39901 accggtttcc actaatccgg aaaacgttgc accgcaaacg ccggaacaga atgctattgc
    39961 tgatggttat gcgcctgata gcccggcacc agtcgttcag caaacatccg cacgcactac
    40021 caccagtaac ggtcataacc ctttccgtaa ctgacgacga cgacggggct tcggccccgt
    40081 ctgaacgtaa ggcaacgtaa agatacgggt tatctgccgc attcttcccc cttctcgctt
    40141 caatttcgac cagccatcct ctattctgat gggtatttac cactggtccc ggaagacaaa
    40201 aatgaaacgg acaaaatcca tacgccacgc atcgttccgc aaaaactgga gcgcacgcca
    40261 tctgacacca gtcgctctcg cggttgccac tgtttttatg ctggctggct gtgaaaagag
    40321 tgatgaaaca gtgtctctct atcaaaatgc tgacgactgt tcagctgcaa acccaggcaa
    40381 aagcgccgaa tgtaccaccg cgtacaacaa tgcgctgaaa gaagccgaac gtactgcgcc
    40441 gaaatacgcc acccgtgaag actgtgttgc tgaatttggt gaaggtcagt gccagcaggc
    40501 accagcccag gctggcatgg caccagaaaa ccaggcgcag gcccagcaat ccagcgggag
    40561 tttctggatg ccgctgatgg ccggttacat gatggggcgt ctgatgggcg gcggcgcggg
    40621 atttgcacag cagccgctgt tctcctcgaa aaacccagcc agcccggctt acggtaaata
    40681 taccgacgcg acgggtaaaa actatggcgc agcccagcca ggccgcacca tgaccgtacc
    40741 gaagacagcc atggcaccaa aaccggcgac cactactacc gttacccgtg gcggttttgg
    40801 tgaatctgtt gccaaacaaa gcactatgca gcgtagcgca accggcacct cttctcgttc
    40861 aatgggtggc tgataccgat ggaaagagtc agtattaccg agcgcccgga ctggcgcgaa
    40921 aaagcccacg aatacggttt caattttcac accatgtacg gcgagccgta ctggtgtgaa
    40981 gatgcttact acaagttgac cctcgcccag gttgaaaagc tggaagaagt caccgccgaa
    41041 ctgcaccaga tgtgcctgaa agtggtggaa aaagtgatcg ccagcgatga gctgatgacc
    41101 aaattccgca ttccaaaacg cacctggagt tttgtgcgtc agtcatggct gactcaccag
    41161 ccatcgcttt attcgcgtct tgatctggca tgggatggca ctggcgaacc taaactgctg
    41221 gaaaataacg ccgatacacc aacgtcacta tacgaggcgg cgttctttca gtggatctgg
    41281 ctggaagatc agcttaacgc cggtaacttg ccggagggca gcgaccagtt taacagtctg
    41341 caagaaaagc taatcgatcg ctttgtcgag ttgcgtgaac agtatggctt ccagttgctg
    41401 catctcacct gctgtcgcga cacggtggaa gatcgcggaa ccattcagta tttgcaggac
    41461 tgcgcgacgg aagctgaaat cgctaccgag ttcctctaca ttgacgatat cgggttaggt
    41521 gaaaaaggtc agttcacgga tttacaggat caggtaattt ccaacctgtt caagctgtat
    41581 ccatgggaat ttatgttgcg tgagatgttc tccaccaagc tggaggatgc aggcgtacgc
    41641 tggctggaac cggcgtggaa gagcattatc tccaacaaag cgcttctacc gctactatgg
    41701 gagatgttcc cgaatcaccc gaacctgctg cccgcttatt ttgcggaaga tgatcatccg
    41761 caaatggaaa aatatgtggt taaaccgatc ttctcccgtg aaggcgcaaa cgtgtcgatc
    41821 attgagaacg gcaaaaccat tgaagcagcg gaaggtccgt atggcgaaga agggatgatt
    41881 gttcagcaat tccacccgtt accgaaattc ggcgacagct atatgctgat tggtagctgg
    41941 ctggtgaacg atcaacctgc cggaattggc attcgtgaag accgtgcgtt gatcacccag
    42001 gatatgtctc ggttttatcc acatattttt attgagtaat caatgatgcc ggatggcatt
    42061 cgccatccgg taattgttaa cctatctgca ccgacagcat actcaggctg cccatttcta
    42121 taccatcgac cggaatggta attggctcct gcccatccca cgcacctaag acatacaaca
    42181 acggcagata gtgctccggc gttgggttcg ataacgcgcc accttcatgg tcaaggtaat
    42241 tcaccagagg atgctgctct accgcccctt gccacgtcag attcgctttc acatactcat
    42301 taaacgacat cgcccaagga tatggtgaac tatcaccgtg ccacttcact gtacgcaggt
    42361 tatgcaccac gttgccactg gcgaccaaca ttattccttc atctcgcagc gctgccagtt
    42421 tgcgccccat ttcgaaatgc caggcggcag gtttgctact gtcgatactc aactgcacca
    42481 tcgggatatc ggcgtcagga tacatcttaa tcagcacgcc ccaggaaccg tggtcaaagc
    42541 cccaggcttc tttatccagc gtcaccggga ccgacgctaa cagctcaacc agatgctgtg
    42601 ccagcacagg cgaacccgga gcaggataat gcgtatcgta cagcgcctgc gggaagccac
    42661 caaagtcatg aatcgtgggc ggcgtctcca tcgcggtcac tcctgttcca cgggtaaacc
    42721 agtgagccga aaccaccaca atcgcttgcg ggcgtggcaa tgtcatcccc aacttctgcc
    42781 agctgcgggt atacaaatta tcttccagca cgttcatcgg actaccgtga cctaaaaaca
    42841 atgctggcat acgtgttgaa gacatgatga tatccttaac taaaggtgtc attttgatat
    42901 cctcacaata cgcttgttcg gcggagtaag aacccggata acaatgatga tgatcatcag
    42961 ttattttgac gatctgcctg aaggtgaaga tttataagga gttgtcgatg tcagtacctc
    43021 tcattctgac catactggcg ggggcagcca cgtttattgg cgcgtttctc ggcgttctcg
    43081 ggcaaaaacc ctcgaaccgc ttactggcgt tttcgctagg atttgcggcg gggatcatgt
    43141 tgctcatctc attaatggaa atgcttcctg ccgcactagc agctgaagga atgtcgcctg
    43201 tgttgggtta tggaatgttt atcttcggtc tgcttggcta ttttggcctg gaccgcatgt
    43261 tgccacatgc gcatccgcag gatttaatgc aaaaatcggt gcagccgttg ccaaaatcga
    43321 tcaagcgcac agccattctg ctcactctcg gcatcagtct gcataacttc ccggaaggga
    43381 ttgccacctt tgtcacggcg agcagcaacc tggagctggg atttggcatc gcactggccg
    43441 tcgcgttgca caatatccct gaaggtctgg cagtggcagg cccggtttat gcggcaacgg
    43501 ggtctaaacg caccgcgatt ctgtgggcgg ggatttctgg actagcagaa attcttggtg
    43561 gtgtgctggc gtggttaatc ctcggtagca tgatttcccc ggtggtcatg gcggcaatca
    43621 tggcggcggt tgcaggaatt atggtggcgc tctcggttga tgaattaatg ccgctcgcca
    43681 aagagattga ccctaataat aaccccagct atggcgtact atgtggaatg tcagtgatgg
    43741 gattcagttt agtgctgcta caaacggcgg gaattggtta aaaacaacat cagaagttgt
    43801 ccagcacatg gacaacttct gccagcttca cgaaagcctt attctacctt ttcggctgtc
    43861 tcttctctcg tactgtttaa gtatttgttc cgcgcttcaa gcagtgcgtc tctgtcctgg
    43921 cgaaacgccc gacaataacg tttcaaatgc aggaaataac ccaccacaac cagagcaatc
    43981 aatacaatcc agtaccaggc gataaacatc cttttaccct taatatttaa caatactaaa
    44041 catttagcgc ataaatttca catatccttt ttcggatata ttcatatggt cgcataataa
    44101 cggaacaatt atatgatttt gatcataatg aaaaagaatg cctgatatta atgaaatttt
    44161 ctcatagaaa gagaagcgga attaacatct tgcatagcag ggcaaaataa aaaccgaagc
    44221 cagctacaac ttcggtttct tgattaaggc agtaattaag cagcggtttt cagctggctt
    44281 tacgctcatg tgcctgacgg tatgccacca ggtcttcaat agtcacgagc gccatattgt
    44341 gtttattggc aaactcaatg cactctggtg cacgcgccat cgtgccatcg tcattagtca
    44401 gctcacacag cacaccagcc ggtttaaagc ctgccagcgt catcaaatca atagtcgctt
    44461 cagtatgacc gccacgcgtc agtacaccac ctgcctgcgc gcgaagtggg aaaacgtggc
    44521 ccgggcgatt cagatcagaa ggttttgcgc catcggcaat cgctgcgcga acggtcgtaa
    44581 tacgatcagc ggcagaaaca ccggtagtca caccttcagc cgcttcaatg gtcacggtaa
    44641 aaccggtgcc ataggcgctg gtgttatttt ctaccatcat tggcagatca agttgtttac
    44701 ggcgatcttc agtgatgcac aggcaaacaa taccgctacc gtggcgaatg gtcagcgcca
    44761 tctgctcaac ggtcatggtt tctgccggga agatcatatc gccttcgttt tcacggtctt
    44821 catcatcaag caccattaca ccgcgtcctt cacgcagcgc agccagtgca ttttcaacac
    44881 gttcgaaagg cgtaccaaaa gaggaaagta gcgtctgatt catggtaaaa aaacctcact
    44941 aaaattatgg ttaccagaat cagggcagtc ttaggagtgg cggcatatag ccaaaataac
    45001 gtgagcgggt ccatgcccga cagaatcgtt actctctccc atccggactc taaccgtcgg
    45061 ccccggaatt acaccggatc tgctgtcctt tgagttcgca cccaaagcgc tcgcgggctt
    45121 tcaactgagt tgatttaccg ccggtgggga atttcgcccc gccctgagaa taagcggatt
    45181 cactataacg ctaatgatta gcggcagcaa cgcatagctt cacataattc tggtttatga
    45241 cttaccctta tcgcactaca atggtactca acacctatca gtacagggaa actacaatga
    45301 ttgacccgaa aaaaattgag caaatcgctc gccaggttca cgaatcaatg cctaaaggaa
    45361 tcagggagtt cggggaagat gtggagaaaa aaatccgcca aaccctacaa gctcagctga
    45421 cgcgcctcga ccttgtaagc cgtgaagagt tcgacgtcca aacgcaagtc ctgttacgga
    45481 ctcgtgagaa actggcgcgg cttgaacaaa gaatgagcga actggaaaat cgcagtactg
    45541 agattaaaaa gcagccagat ccagagactc tccccccaac gctgtaaaca tttgtcttta
    45601 atgcactgta aattatgaat taatcttcta atttacagtg ctttctatta ccttatcgct
    45661 taattccatt cagaatatca tcacaaa
//
LOCUS       DADMKF010000011        44448 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.011, whole genome shotgun sequence.
ACCESSION   DADMKF010000011 DADMKF010000000
VERSION     DADMKF010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 44448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44448
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.011"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(<1..716)
                     /locus_tag="JNC24_000594"
     CDS             complement(<1..716)
                     /locus_tag="JNC24_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAY9157435.1"
                     /translation="MAQLSLQHIQKIYDNQVHVVKDFNLEIADKEFIVFVGPSGCGKS
                     TTLRMIAGLEEISGGDLLIDGKRMNDVPAKARNIAMVFQNYALYPHMTVYDNMAFGLK
                     MQKIAKEVIDERVNWAAQILGLREYLKRKPGALSGGQRQRVALGRAIVREAGVFLMDE
                     PLSNLDAKLRVQMRAEISKLHQKLNTTMIYVTHDQTEAMTMATRIVIMKDGIVQQVGA
                     PKTVYNQPANMFVAGFIGSSA"
     gene            complement(730..1389)
                     /gene="pgmB"
                     /locus_tag="JNC24_000595"
     CDS             complement(730..1389)
                     /gene="pgmB"
                     /locus_tag="JNC24_000595"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAY9157436.1"
                     /translation="MKLQEVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNSQERAQLAYRKNLLYVHSLRELTVNAVLPGIRSLL
                     ADLRAQQISVGLASVSLNAPTILDALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGITGAQLLLPSTDSLTWSRLSAFWQN
                     V"
     gene            complement(1386..3653)
                     /gene="ycjT"
                     /locus_tag="JNC24_000596"
     CDS             complement(1386..3653)
                     /gene="ycjT"
                     /locus_tag="JNC24_000596"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HAY9157437.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDVVGMEIAINGEVFSLSREAWQRELDFASGELR
                     RSVVWRTSNGTGYTIASRRFVSADQLPLIVLEITITPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCKVSGDVQQCYTAKERRLLQHTS
                     AQLHAGETVTLQKLVWIDWRDDRQAVLDEWGSASLRQLEMCAQQSYDQLLAVSTENWR
                     QWWQKRRITVNGGEVHDQQALDYALYHLRIMTPAHDERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMAYYNVQQALSIARQFGCSDDA
                     FIHRAEMFLKELRLPEIQPDGVLPQDDSFMAKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSAASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGGSVRDGELHLNPA
                     LPEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLNGQLISVAEESVFCLGDF
                     ILPFNGTAATHQEDE"
     gene            complement(3650..4705)
                     /locus_tag="JNC24_000597"
     CDS             complement(3650..4705)
                     /locus_tag="JNC24_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAY9157438.1"
                     /translation="MKSAMTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSR
                     LSQAQALAEKYGNASVWDDPQAMLLAVKPDVVSVCSPNRFHYEHTLMALEAGCHVMCE
                     KPLAMTPEQAREMCDTARKLGKVLAYDFHHRFALDTQQLREQVTNGVLGEIYVTTARA
                     LRRCGVPGWGVFTNKELQGGGPLIDIGIHMLDAAMYVLGFPAVKSVNAHSFQKIGTQK
                     SCGQFGEWDPATYSVEDSLFGTIEFHNGGILWLETSFALNIREQSIMNVSFCGDKAGA
                     TLFPAHIYTDNNGELMTLMQRKMADDNRHLRSMEAFINHVQGKPVMIADAKQGYIIQQ
                     LVAALYQSAETGTRVEL"
     gene            complement(4715..5503)
                     /locus_tag="JNC24_000598"
     CDS             complement(4715..5503)
                     /locus_tag="JNC24_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAY9157439.1"
                     /translation="MKIGTQNQAFFPENILEKFRYIKEMGFDGFEIDGKLLVNNLEEV
                     KAAIKETGLPVTTACGGYDGWIGDFIEERRLNGLKQIERILEALAEVGDKGIVVPAAW
                     GMFTFRLPPMTSPRSLDGDRKMVSDSLRVLEQVAARTGTVVYLEPLNRYQDHMINTLA
                     DARRYIVENDLKHVQIIGDFYHMNIEEDNLAQALHDNRDLLGHVHIADNHRYQPGSGT
                     LDFHALFEQLRADNYQGYVVYEGRIRAEDPAQAYRDSLAWLRTC"
     gene            complement(5521..6573)
                     /locus_tag="JNC24_000599"
     CDS             complement(5521..6573)
                     /locus_tag="JNC24_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HAY9157440.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKVRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPEGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAHRCDIARRHGADFCLNPIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSAFRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            complement(6604..7207)
                     /locus_tag="JNC24_000600"
                     /pseudo
     CDS             complement(6604..7207)
                     /locus_tag="JNC24_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415828.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
     gene            complement(7194..8075)
                     /locus_tag="JNC24_000601"
     CDS             complement(7194..8075)
                     /locus_tag="JNC24_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAY9157441.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(8096..9406)
                     /locus_tag="JNC24_000602"
     CDS             complement(8096..9406)
                     /locus_tag="JNC24_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY9157442.1"
                     /translation="MIKSKIVLLSALVSCALISGCKEENKTHVSIEFMHSSVEQERQA
                     VISKLIARFEKENPGITVKQVPVEEDAYNTKVITLSRSGSLPEVIETSHDYAKVMDKE
                     QLIDRKTVATVISNVGEGAFYDGVLRIVRTEDGSAWTGVPVSAWIGGIWYRKDVLAKA
                     GLEEPKNWQQLLDVAQKLNDPANKKYGIALPTAESVLTEQSFSQFALSNQANVFNAEG
                     KITLDTPEMMQALTYYRDLAANTMPGSNDMPGSNDIMEVKDAFMNGTAPMAIYSTYIL
                     PAVIKEGDPKNVGFVVPTEKNSAVYGMLTSLTITAGQKAEETEAAEKFVTFMEQADNI
                     ADWVMMSPGAALPVNKAVVTTATWKDNDVIKALGELPNQLIGELPNIQVFGAVGDKNF
                     TRMGDVTGSGVVSSMVHNVTVGKADLPGTLQASQKKLDELIEQH"
     gene            complement(9441..10951)
                     /locus_tag="JNC24_000603"
                     /pseudo
     CDS             complement(9441..10951)
                     /locus_tag="JNC24_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
     gene            complement(11163..11477)
                     /gene="pspE"
                     /locus_tag="JNC24_000604"
     CDS             complement(11163..11477)
                     /gene="pspE"
                     /locus_tag="JNC24_000604"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HAY9157443.1"
                     /translation="MFKKGLLALALVFSLPVFAAEHWIDVRVPEQYQQEHVQGAINIP
                     LKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKEILSEMGYTHVENAGGLKDIAMPKV
                     KG"
     gene            complement(11552..11773)
                     /gene="pspD"
                     /locus_tag="JNC24_000605"
     CDS             complement(11552..11773)
                     /gene="pspD"
                     /locus_tag="JNC24_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HAY9157444.1"
                     /translation="MNTRWQQAGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(11782..12141)
                     /gene="pspC"
                     /locus_tag="JNC24_000606"
     CDS             complement(11782..12141)
                     /gene="pspC"
                     /locus_tag="JNC24_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HAY9157445.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(12141..12365)
                     /gene="pspB"
                     /locus_tag="JNC24_000607"
     CDS             complement(12141..12365)
                     /gene="pspB"
                     /locus_tag="JNC24_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HAY9157446.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRDELSQSEQQRLA
                     QLADEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(12419..13087)
                     /gene="pspA"
                     /locus_tag="JNC24_000608"
     CDS             complement(12419..13087)
                     /gene="pspA"
                     /locus_tag="JNC24_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HAY9157447.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVNDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            13254..14231
                     /gene="pspF"
                     /locus_tag="JNC24_000609"
     CDS             13254..14231
                     /gene="pspF"
                     /locus_tag="JNC24_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HAY9157448.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNAVERSVYRHGTSDYPLDDIIIDPFKRRPPEDAIAVSETTSLPTLP
                     LDLREFQMQQEKELLQLSLQKGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(14349..15614)
                     /gene="puuE"
                     /locus_tag="JNC24_000610"
                     /pseudo
     CDS             complement(14349..15614)
                     /gene="puuE"
                     /locus_tag="JNC24_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415818.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
     gene            complement(15652..16932)
                     /locus_tag="JNC24_000611"
     CDS             complement(15652..16932)
                     /locus_tag="JNC24_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HAY9157449.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESITCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLELLDANAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTAKGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARDPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSRMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPYYPFPGGRTLRVPFTAMGAAWYSLRDRLGV"
     gene            complement(16934..18424)
                     /gene="puuC"
                     /locus_tag="JNC24_000612"
                     /pseudo
     CDS             complement(16934..18424)
                     /gene="puuC"
                     /locus_tag="JNC24_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415816.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
     gene            complement(18574..19131)
                     /gene="puuR"
                     /locus_tag="JNC24_000613"
     CDS             complement(18574..19131)
                     /gene="puuR"
                     /locus_tag="JNC24_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HAY9157450.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(19158..19922)
                     /gene="puuD"
                     /locus_tag="JNC24_000614"
     CDS             complement(19158..19922)
                     /gene="puuD"
                     /locus_tag="JNC24_000614"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HAY9157451.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPGSPSNVQPHLYGENGDEPDADPGRDLLSMAIINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITACQHHIAEKQRL"
     gene            20134..21495
                     /locus_tag="JNC24_000615"
     CDS             20134..21495
                     /locus_tag="JNC24_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HAY9157452.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRDAEGNLQPPC
                     APGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIPADGAVAEASPGQFEINLYH
                     TDNVLEACDDALALKRLVRLMAGKHKMHATFMAKPYEEHAGSGMHIHISMQNNRGENV
                     LSDTEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRRFQPGMYVPTQASWGHNNRT
                     VALRIPCGDRHNHRVEYRVAGADANPYLVMAAIFAGILHGLDNELPLQEEVEGNGLEQ
                     EGLPFPIRQSDALGEFIENDHLRRYLGKRFCHVYHACKNDELLQFERLITETEIEWML
                     KNA"
     gene            21644..21808
                     /locus_tag="JNC24_000616"
     CDS             21644..21808
                     /locus_tag="JNC24_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9157453.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            21798..23183
                     /gene="puuP"
                     /locus_tag="JNC24_000617"
     CDS             21798..23183
                     /gene="puuP"
                     /locus_tag="JNC24_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HAY9157454.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YEGDRTPVSET"
     gene            23317..23562
                     /locus_tag="JNC24_000618"
     CDS             23317..23562
                     /locus_tag="JNC24_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HAY9157455.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVAEAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            23875..25518
                     /gene="sapA"
                     /locus_tag="JNC24_000619"
     CDS             23875..25518
                     /gene="sapA"
                     /locus_tag="JNC24_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein SapA"
                     /protein_id="HAY9157456.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            25515..26480
                     /gene="sapB"
                     /locus_tag="JNC24_000620"
     CDS             25515..26480
                     /gene="sapB"
                     /locus_tag="JNC24_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapB"
                     /protein_id="HAY9157457.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATMELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            26467..27357
                     /gene="sapC"
                     /locus_tag="JNC24_000621"
     CDS             26467..27357
                     /gene="sapC"
                     /locus_tag="JNC24_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HAY9157458.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            27357..28349
                     /gene="sapD"
                     /locus_tag="JNC24_000622"
     CDS             27357..28349
                     /gene="sapD"
                     /locus_tag="JNC24_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HAY9157459.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            28351..29157
                     /gene="sapF"
                     /locus_tag="JNC24_000623"
     CDS             28351..29157
                     /gene="sapF"
                     /locus_tag="JNC24_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HAY9157460.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            29225..29578
                     /locus_tag="JNC24_000624"
     CDS             29225..29578
                     /locus_tag="JNC24_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HAY9157461.1"
                     /translation="MMDKIKSNARDLRRNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDTRRTSWLESQGWTVLRFWNNEIDCNEEAV
                     PEIILQELNRRSPSP"
     gene            29946..30734
                     /gene="fabI"
                     /locus_tag="JNC24_000625"
     CDS             29946..30734
                     /gene="fabI"
                     /locus_tag="JNC24_000625"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HAY9157462.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            30879..32005
                     /locus_tag="JNC24_000626"
                     /pseudo
     CDS             30879..32005
                     /locus_tag="JNC24_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309887.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
     gene            32073..34007
                     /gene="rnb"
                     /locus_tag="JNC24_000627"
     CDS             32073..34007
                     /gene="rnb"
                     /locus_tag="JNC24_000627"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HAY9157463.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAKKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLAIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTALDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWQPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTGTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRGIIARPVA"
     gene            complement(34085..34188)
                     /locus_tag="JNC24_000628"
                     /pseudo
     CDS             complement(34085..34188)
                     /locus_tag="JNC24_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            34242..36232
                     /gene="pdeR"
                     /locus_tag="JNC24_000629"
                     /pseudo
     CDS             34242..36232
                     /gene="pdeR"
                     /locus_tag="JNC24_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
     gene            36379..36552
                     /locus_tag="JNC24_000630"
     CDS             36379..36552
                     /locus_tag="JNC24_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9157464.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKAPWEI"
     gene            36642..37391
                     /locus_tag="JNC24_000631"
     CDS             36642..37391
                     /locus_tag="JNC24_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAY9157465.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            37660..37878
                     /gene="osmB"
                     /locus_tag="JNC24_000632"
     CDS             37660..37878
                     /gene="osmB"
                     /locus_tag="JNC24_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HAY9157466.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(38004..38330)
                     /gene="yciH"
                     /locus_tag="JNC24_000633"
     CDS             complement(38004..38330)
                     /gene="yciH"
                     /locus_tag="JNC24_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HAY9157467.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGVVRIQRQTSGRKG
                     KGVCLITGVDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(38330..39067)
                     /gene="pyrF"
                     /locus_tag="JNC24_000634"
     CDS             complement(38330..39067)
                     /gene="pyrF"
                     /locus_tag="JNC24_000634"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HAY9157468.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNRDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLVDLGMTLSPADYAERLAALTQK
                     CGLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALSAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            complement(39260..40429)
                     /gene="lapB"
                     /locus_tag="JNC24_000635"
     CDS             complement(39260..40429)
                     /gene="lapB"
                     /locus_tag="JNC24_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HAY9157469.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYRCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            complement(40436..40744)
                     /gene="lapA"
                     /locus_tag="JNC24_000636"
     CDS             complement(40436..40744)
                     /gene="lapA"
                     /locus_tag="JNC24_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HAY9157470.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGVAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            complement(40893..41657)
                     /gene="pgpB"
                     /locus_tag="JNC24_000637"
     CDS             complement(40893..41657)
                     /gene="pgpB"
                     /locus_tag="JNC24_000637"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HAY9157471.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFCIKAAFVLFAILAAVIIVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            41827..42417
                     /gene="ribA"
                     /locus_tag="JNC24_000638"
     CDS             41827..42417
                     /gene="ribA"
                     /locus_tag="JNC24_000638"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HAY9157472.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            complement(42481..>44448)
                     /gene="acnA"
                     /locus_tag="JNC24_000639"
     CDS             complement(42481..>44448)
                     /gene="acnA"
                     /locus_tag="JNC24_000639"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HAY9157473.1"
                     /translation="MLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLRKHGV
                     VEKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSEDQVE
                     LVEKYAKAQGMWRNPGDEPIFTSTLELDMNDVEASLAGPKRPQDRVALPDVPKVFAAS
                     NELEVNATHKDRQPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLLAKKA
                     VILGLKRQPWVKASLAPGSKVVSDYLAKAKLTPFLDELGFNLVGYGCTTCIGNSGPLP
                     DPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNINLAS
                     EPIGHDRKGDPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEINVTRS
                     DTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGSIKPD
                     SPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTRHLPD
                     SDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESFERIH
                     RSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADGSQEV
                     VPCRCRIDTATELTYYQNDGILHYVIRNMLK"
BASE COUNT        10458 a        11768 c        11220 g        11002 t
ORIGIN      
        1 gccgatgatc caataaatcc ggcaacaaac atattcgctg gttggttata aaccgttttc
       61 ggcgcaccaa cttgctgaac aatcccatcc ttcataatca caatccgcgt cgccatggtc
      121 atcgcttcgg tctgatcgtg ggtcacgtag atcatggtgg tattcagctt ctggtgcagc
      181 ttgctgatct ctgcgcgcat ttgcacgcgc agcttggcat caaggttaga gagcggttca
      241 tccattaaaa acacgcccgc ttcgcgcaca atcgcccgcc cgagcgccac acgctggcgt
      301 tgcccgccgg aaagcgcgcc cggcttgcgt ttcaggtact cacgcaggcc gagaatttgc
      361 gccgcccagt tcacccgctc atcaatcacc tctttggcga ttttttgcat cttcaggcca
      421 aacgccatgt tgtcgtaaac cgtcatatgc ggatacaacg cgtagttctg gaacaccatc
      481 gctatattgc gtgctttggc tggaacgtca ttcattcgtt tgccgtcgat caacagatcg
      541 ccgccgctga tctcctcaag cccggcaatc atgcgcaggg tggtcgactt accgcagcct
      601 gacggaccga caaacacgat gaactcttta tcggcaattt ccaggttgaa gtccttcacc
      661 acatgcacct ggttatcgta gattttttga atatgttgta acgaaagctg agccatgttg
      721 attcctttgc tatacgtttt gccagaaggc cgataaccgc gaccaggtca gggagtccgt
      781 tgaaggcaat agtaattgcg ccccggttat gcccgcgccg atccccaccg agcgcatacc
      841 gctggcgtta atggcgtcaa tgcccgcctg cgcatcttca atgccgatac atgcctgcgg
      901 cggcacgccc agcccggcac aggcagcgag aaagatttcc ggatccggtt tcgagttttt
      961 aagttgggaa gcatccgcgc agaaggtgaa aaactcacgc agctccagtg catctaaaat
     1021 cgtcggcgca ttgagggaga cagaagccag ccctaccgag atctgttgtg cgcggagatc
     1081 tgccagcaaa gagcgaatgc cgggtagaac agcgttgacc gtcaactcgc gcaatgagtg
     1141 gacatagagc agatttttgc gatacgccag ttgcgccctc tcctgcgagt taaagtcgcc
     1201 ctctttgccc ccgtgttgca gaatgcgccg cagagactca tcgcggctga tcccttttag
     1261 ggattcgtta aactgcgcat caatgctgat gccaatttca gcggcaatct gctgccacgc
     1321 ctggaaatgc agatgcgcgg tatcggtgat tacaccatcc agatcgaaaa ttacctcttg
     1381 cagtttcatt catcctcctg atgtgtggca gcggtcccat tgaagggcaa aataaaatca
     1441 cccaaacaga aaacagattc ttcagccacg gatataagct gaccgttcaa acgcagtgaa
     1501 acgggggcag aagttcgaat cgcaatacgc tgcgcgtcaa gagtgacctg taattcgcag
     1561 ccctgccaga acagagggaa agacaactgt tgccactgct caggtaacgc cggattgaga
     1621 tgcaattcac cgtcacgcac gctccccccg gcaaaaccct gaatcgcccc cagccagata
     1681 gcgccagttg cggcagcatg gataccatca tcacaactat gcggatcagc accaagatcg
     1741 atttcagtcc cctcgcgcca gaactgataa ctttgggtca gcaggccaca gcgtgcggca
     1801 acaatgccgt ggattgcttt acttaatgac gagtcgtgaa tagtgcgcgg ttcataaaat
     1861 tgcagattgg caagacacga tgccgctgag aactgctccg gcagcatgta attgagcatc
     1921 accacatcag cttgtttgag gatttgcatc tcgttcactt ctgcgcgtga ataatccagc
     1981 agaatggttt gcttccccgc cgccgctttg tatttcgcaa gattaatcgc cggcttagcc
     2041 ataaacgaat catcctgcgg caaaacgccg tcgggctgaa tttctggcag ccgcagttct
     2101 ttaaggaaca tttcggcacg atgaataaac gcgtcgtcgc tacagccaaa ctggcgggca
     2161 atactcagcg cctgctggac gttgtaatac gccatatagc tggtgaaggc gttattattg
     2221 acatgttcgg tatattcgtc aggcccaata acatcatgaa tttccagacg gtcgttaacc
     2281 ctcaccgcgc ggctaatcca gaactttgca gtttccagaa gtagcgccat gccttcatgc
     2341 gcaatgaaac tttcatcccc cgtggtctgc cagtattgaa taaccgccca ggcgatatcg
     2401 gccaccagat gatgttccgc ctgcgccgag gccacttttt gccgcagccc ggtgcgaatg
     2461 ttaatggcgg caaattccgg cgtctcttct tcgccgctgc gcgcgctttc ccacggaaat
     2521 agcgcgccct gccagccgtt gcgtcgcgct ttctcctgcg cgcctggcaa gttgtgccag
     2581 cgataacgca gtaaacttcg ggcaaccgtc ggatcgctaa acagatgaaa cggtaacaaa
     2641 aatacttctg tatcccagaa aacgtggcct ttgtagcctt cgccggttaa gccttttgcc
     2701 gcaatgctgc tgcgctcgtc gtgggcaggc gtcatgatgc gcagatgata aagcgcataa
     2761 tctaacgctt gctgatcgtg cacttcgccg ccatttaccg tgatacgacg tttctgccac
     2821 cattgacgcc agttttctgt tgatactgca agaagttggt cgtaactctg ctgcgcgcac
     2881 atttcaagct ggcgaagcga cgcgctgccc cactcgtcta aaacggcttg cctgtcatcc
     2941 cgccagtcga tccacaccag tttttgcaac gtcactgtct cgcctgcatg aagctgcgca
     3001 ctggtatgtt gcagtaaacg gcgctctttg gcggtatagc attgctgcac atcaccgctc
     3061 accttacagc aacagctgat ggccacatca ctgcgtccat cctgggtggt gtagatcccc
     3121 tgcatcaaat gctgaccaaa cacccgcacc tgggtttcgt cgagatgctg gcgaccgtgg
     3181 ttggtctgcg tggcgtcaat gcctgttgaa atcagcactg acgcgtcggc gtccagtggc
     3241 gtaatagtga tttccagcac aatgagcggc agttggtctg ccgaaacaaa gcgacggctg
     3301 gcgatggtgt aacctgtgcc gttgctggta cgccagacaa cgctacggcg taattctcca
     3361 ctggcaaagt caagttcacg ctgccaggct tcgcgggata acgagaaaac ctcaccattt
     3421 atggcaatct ccatccccac gacatcaggc aggttcacca gttcgttgat ttcacctttt
     3481 cccgcccgat gatacagccc cgccagatac atccctcgcg tctggcgcgt gtaatcttct
     3541 tcatggctgg cgcgaagccc aagatagccg ttcccctgcg ccatcagcga tgcatacttg
     3601 ttcagggtat gctggctgaa atggggttct gataacgtta ctggcctggt cataattcca
     3661 cacgcgtccc tgtttctgcg gattgataca acgccgccac cagttgctgg atgatgtacc
     3721 cctgcttggc gtcggctatc atcacgggct tgccctgtac gtgattgata aaggcttcca
     3781 tgctgcgcaa atggcggttg tcgtctgcca ttttccgttg catcagcgtc attaattcac
     3841 cgttgttatc ggtgtagata tgtgctggaa acagcgtcgc accagcttta tcaccacaaa
     3901 agctgacgtt cataatcgac tgttcgcgga tgttgagtgc aaatgacgtt tccagccaca
     3961 gaatgccgcc gttatgaaat tcaatggtgc caaacagcga atcttcgacg ctgtaagttg
     4021 ccggatccca ctcaccaaat tgaccacagc tcttttgcgt gccgatcttt tgaaagctat
     4081 gcgcattcac gcttttcacc gccggaaagc ccagcacata catcgcagca tccagcatat
     4141 gaatgccgat gtcgatcagc gggccaccac cctgcagttc tttattggta aagacacccc
     4201 agccgggaac gccgcagcga cgcagggcgc gggcggtggt aacgtaaatc tctcccaaaa
     4261 cgccgttggt cacctgttca cgcagctgtt gcgtatcgag cgcaaaacga tggtgaaagt
     4321 cgtaggccag caccttgccc agtttgcgcg cggtatcgca catttcccgc gcctgttctg
     4381 gcgtcatggc gagcggtttt tcgcacatca catggcagcc cgcttccagt gccatcaggg
     4441 tatgttcgta atgaaaacgg ttaggtgagc agacgctaac cacatcaggt ttcaccgcca
     4501 gcagcatggc ctgcggatcg tcccacacgg atgcattccc gtatttttct gccagcgcct
     4561 gcgcctggga aaggcggctg tcacagacag ccaccagttc cagatcgttg cgggtgcagt
     4621 agtacgaagc atgaacctta tccgccacct ggcctgcgcc tattatcgcg acccgcagcg
     4681 gagagcttgt cattgcactt ttcacaaaga cctcttagca ggtacgcaac caggccaacg
     4741 aatcacggta cgcctgggca ggatcttccg cccggatacg cccttcgtac actacatagc
     4801 cctgatagtt atccgcgcgc agctgttcaa acagcgcgtg gaaatccagg gtgccgctgc
     4861 ccggctggta gcgatggtta tccgcaatat gcacatgacc gagcaggtcg cggttgtcat
     4921 gcagcgcctg cgccaggtta tcttcttcga tattcatgtg atagaaatcg ccgataatct
     4981 gtacatgttt aagatcgttt tcgacgatgt aacggcgggc atcggcgagg gtgttgatca
     5041 tatgatcctg atagcggttt aacggttcga gatacaccac tgttccggta cgcgcggcga
     5101 cctgttccag tacgcgcagg gaatcactca ccattttgcg gtcgccgtcc aggctacgcg
     5161 gcgaggtcat cggcggtaag cggaaggtaa acatgcccca cgctgccgga acgacgatac
     5221 ctttatcccc cacttctgcc agcgcttcga gaatgcgttc gatctgcttt aagccattaa
     5281 gacgacgctc ttcgataaag tcgccaatcc aaccgtcata gccaccgcag gcggtggtca
     5341 ccggtaaacc ggtttctttg attgctgctt tgacttcttc gagattgttg accagcagtt
     5401 tgccgtcaat ctcaaaacca tcgaagccca tctctttgat ataacgaaat ttctccagaa
     5461 tgttttccgg aaagaatgcc tgattctgtg tgcctatttt cattttttca ttcctttgaa
     5521 ttaaaacgta acgcccattt tgatgctctg ttccggatgc tgatcgacat actgcatgta
     5581 gctttccggg ctattggcaa aggtcactac cgggtcgatt aaatcttcgc aattgagata
     5641 accgttcatc agcagttccc agcaggtttc ttcaatacgc ttacggctcc agcgcggata
     5701 atccgggttc ggttcgctgc acgcgcggga gaaaacaatt ttggcgttat tgaaatgcgc
     5761 ttcgcgtccg aggttaaaac cttcagcaaa tggtttggca aacgcaacat aggagatggt
     5821 gccaccgtag gccagaccgc ggaacgccga ttgcagcgcg tcggcatagc cgctggtttc
     5881 gataatcaca tcggcaccct gcttgccggt cagcgttttg atctctttgc ctacgtcagt
     5941 accaatgggg ttaaggcaga aatccgcgcc gtggcgacgg gcaatatcac agcgatgagc
     6001 gataggatcg acgccaatca cgaccgacgc gccagcacgt ttagccagtt gaatggcaat
     6061 ttgaccaatc gcgccaagcc ctaccaccac aacaaaatca cccacgcgca cgttggcatc
     6121 gcgcacgccg ctcatggcaa actgcgccgg gtcgtaacaa acggcgtttt tccacgagct
     6181 gccttccggc attttgcgca gcttgtagtt attcaccgcg ttaatgatga ccgtctcgga
     6241 gagcgggccg tagccgcata cgctgtcgcc caccgcgtag tcggtaacgt cgctgccgca
     6301 ctcgataatg tcgccaacca ccatgtttcc aagctggaat ttgccaaact caatgccgcg
     6361 cggcgcatct gccggacgcg gagtgaacat ctgccattcg ccgttaaagt cttcatcgat
     6421 aaacgggctg gcggcgcgga agtcgaccac ttccgttccg tgtttcggtg cgccgaaacg
     6481 cacgcggacc ttcacttcat tagctaaaat cgcccgatct tcatactcaa ccagcgcagc
     6541 aacacgcggt gctgtggcta ctaacttttt catttaaaac tccttgtaaa gaaactggcc
     6601 gctttagccc ttcacgccac cggcggtcaa accactctta ataaaacgtt ccgaaagcgc
     6661 atacataatc accaccggca atgcggtcac cagtgaggcc gccatcatcc gtccccagat
     6721 gtaatctggc gtactgaaca gcgcgttcag gcccaccggt aaggtgaaat tgccggcgct
     6781 ggagaggaaa atcgacgcaa acagatagtc gttccacgcc accataaagc agtagacgaa
     6841 cacggatatc agcccggaca tcgccaacgg cacggtgatg cggaagataa tttgcagacg
     6901 gttgaggcca tccatcatcg ccgcttcttc gatctcatcg gggatggtgt cgaagtagct
     6961 tttcagcata aacacggcgg tgggcagagt ttgcgtcacc atggtgatga tcagcgccat
     7021 ctcagtcgac gtaatgctcc agcgtccact gctgcggcag cagcgtagga tgcagtgaga
     7081 tcgcctcttt cgcgcccttg aacgaggtca tcagcatcac aaaaaatgga aacagcgtga
     7141 tgatgagaaa cagcgccagc ccgcagtaaa aaccgatgcg actgagtgtg cgtttatttg
     7201 ttgccattga ggttcacccg tttcctggtc agcagaatga cagcgaaaat aatgatgaag
     7261 agcaccaccg aaatcgccgc cgctttccct aagtcattaa aggcaaaggc ggttttgtag
     7321 agatagacgc cgagaatatc gaccttggtg gtcagcagat aaacatcagc aaacatgtag
     7381 aacatccaga tagtgcgcag ggtcaccacc gtcgccagga ccggcataat tgcgggcagc
     7441 gtgacgatgc gaaaccgctg ccaggcatta gcgccatcca tctctgccgc ttcgtacagc
     7501 gatttgtcga tggtctgcaa aatcgccaga aacgagataa aggcatacgg gaagtagcgc
     7561 cagatggcga acagcaccac cagcacaaag ctactgcccg gattatcgaa ccacagcggt
     7621 gcctgctcat agagatgcag aagatcgacg ccgaggtagt tcacaatgcc gtagccgttg
     7681 ttgaacatgt atttccaggc aaacaccagc gaaatggacg gcgttacgta ggagaggatc
     7741 accagcgaac gcgcggtttt gcgcaggcgg aactcgcggt tgaaaaacat cgccactgcc
     7801 agcccgagca cggtgctgcc cgccaccacc agcgcggtat accagaccgt catccacagc
     7861 gagtgccaga agccgggatc ggagaggata cgcacatagt tactcacccc aacaaaggtt
     7921 gactcgatgt tgggattgag cggcagacgt aaaaaactga tttcgatatt cgacaccatt
     7981 ggccaggcca ccagaccacc cagcagtaat aagctgggcg cgagaagcag cagcgcaaag
     8041 ggcatatcgg aacgacctga aaaaagcctg ttcattgcgg attcctgtcc tgggtttagt
     8101 gctgttcgat cagttcatcc agctttttct ggctcgcttg cagcgtgcca gggagatcgg
     8161 ctttacccac ggtgacgtta tgcaccatgg agctcaccac gccagaaccc gtcacatcac
     8221 ccatgcgggt aaagttttta tcccctactg cgccaaagac ctgaatattt ggcagttcac
     8281 cgattagctg attcggtagt tcacccagcg ccttaataac gtcgttgtct ttccaggtgg
     8341 cggtagtgac aaccgcttta ttcaccggca gcgcagcacc tggcgacatc atcacccagt
     8401 cggcaatgtt gtctgcctgc tccataaagg tgacaaattt ttctgctgct tccgtctctt
     8461 cggccttttg cccggcggta atggtcagcg aggtcaacat gccgtagacc gcagagtttt
     8521 tctcggttgg caccacgaaa ccgacgtttt tcgggtcgcc ttctttaatt acagccggaa
     8581 ggatataggt ggagtaaatc gccatcggcg cggtgccgtt cataaaggcg tctttcactt
     8641 ccatgatgtc gttagaaccc ggcatgtcgt tagaacccgg catagtgttg gcagcaaggt
     8701 cgcggtaata ggtcagtgcc tgcatcatct ctggtgtatc aagggtgatt ttgccttcgg
     8761 cgttaaagac attagcctgg ttggataacg caaactggga gaaggattgt tccgtcaaca
     8821 cgctttctgc tgtaggcagc gcaatgccgt attttttatt cgccgggtca ttcagtttct
     8881 gtgcaacgtc cagtagctgt tgccagtttt tcggctcctc cagccccgct tttgccagca
     8941 catctttgcg ataccagatg ccgccaatcc aggcgctgac gggaacaccg gtccaggcac
     9001 tcccatcttc ggtgcgcaca atacgcagta cgccatcgta aaacgcgcct tcaccaacgt
     9061 tgctgatgac cgtggcaacc gtcttgcgat cgataagctg ctctttatcc ataactttgg
     9121 cgtagtcatg gctggtttcg atcacttccg gcagcgagcc gctacgtgaa agggtgatga
     9181 ctttggtgtt ataagcgtcc tcttccacgg gcacttgttt aacggtgata cctgggtttt
     9241 ctttttcaaa acgggcaatc aatttgctga taactgcctg acgctcctgc tccaccgaag
     9301 aatgcataaa ttcgatggat acatgggttt tattttcttc tttacagcct gaaatcaggg
     9361 cgcaggaaac cagtgctgat aacagcacga tttttgattt aatcatgaga tgttcctttt
     9421 tatttaatcc acataacctg ttaggggcgc agcgttattt cattacccaa tagatcaact
     9481 tcactaatca gatcccgacc atgcagatta gtaatattta catgctgaat atttttactg
     9541 acattaaaca atccagtcag acaatcacca tcagcgttac ttctttgaat acgcattacg
     9601 gatgaattaa tcgtatcgat ggtaaaatta ttatccggat gaaactcgtt atggctgcga
     9661 cgaagtgtaa ttaaacgcga caactcatga tataccgcat gccttaatgt agcttcatcg
     9721 ttcagttctc gggttatctc tttactgtga tattttttac ggttaatcgc acggttatat
     9781 ccgagttttt cgacacctgc gtaatcatta cgcgagccaa gaatactttg aatatatatc
     9841 gctggcacac cggggaaact taacaaaatc gcatgggcga gaataaacct ggcgcaacgt
     9901 tcttcatcgc tactctcacg gcggcttaac gcatccatat gtgagaggcg aggaagttaa
     9961 accaggtggt gttgctggaa ggtagtgtca ggttttgcgc ccacgcacaa agcgcctcaa
    10021 cgttctgttt ttgcaccgca tgcagcacca gtggcggcag cgagaactgg tacaccatat
    10081 gtgcttcgtc atcgcctgcg ccaaagtaag caatgttgtc tttatgcgga acattggtct
    10141 cggtaatgat cactgtacct ggcgcaacgt tatcaataat cgaccgtaac agtttgataa
    10201 tcagatgtgt tttttccaga tggatgcagc ttgttcccgg ctctttccac ataaagccaa
    10261 cggcatccag gcggacatac tcagcacctt tcgcaaggta acagagtaaa acatccacca
    10321 tcgccagcaa cacttcaggg ctacggtaat tcaggtcaat ttgatcgtca ctaaaggtgg
    10381 tccataaatg gcgcgttgaa tgatcgcgca tctggaatgg cgttaataac ggtaacgcac
    10441 gcgggcgagt gacggcgctg agatcggttt gcggatcaac cgcaataaaa aaatcttcaa
    10501 aacctggctg ctgttgtaaa tagtttttaa accattcact ttttgccgac atatggttgc
    10561 agacaaaatc aaacattaaa tgactgcatt caccgagttg ctgaatatcc tgccactccc
    10621 ccgcttcact ggcgacctga tgataatcaa ttaccgaaaa cccatcatca gatgaacatg
    10681 gataaaaagg taataaatga acatgtgaaa aaatgctttg cagccattga tggtaaaact
    10741 gattaaatgt gggtaatggt tttaaatcat tgctgtgaaa ttgatcggca taggtaatta
    10801 acacgacatc actttcatcc cagtgttttt tacgtcgctg tgtaattaat cgtttcgcac
    10861 tctcaagacg cgttacaagt ttctgtaaat gagttgcggt aaatgttcca ccgtagattt
    10921 cgtccaggta atccgtaatt ttctgtttca tgttgccttt ttgtggtagc ggtcccatga
    10981 aacgagagac tactccgcag aaaataaact gtcaattgag cacaagggtt ttagtcacgc
    11041 aggaaatcat tttttaccag ggaaaaagcg tatttgtgag ctgacgcaaa taatctatgg
    11101 ggatgtgaat aaggtgtgac agaaaaaaaa cggcgcataa gcgccgctca tggtgaattc
    11161 ttttaacctt tgaccttcgg cattgcgatg tctttcaggc caccggcgtt ctcaacgtgg
    11221 gtatatccca tctcgctaag gatctctttt gcttgccctg actggcgtcc ggcattgcaa
    11281 tacactttca cggtgtcgtt tttatccgga acggcggtgg caatgcgctc tttcacttcc
    11341 ttcaggggaa tattgatggc cccctgaacg tgctcttgct gatactgctc tggaacacga
    11401 acatcgatcc agtgttcagc ggcgaaaacg ggcagtgaaa acaccagtgc cagagcaagt
    11461 aagccttttt taaacatgta agcgtccttc tatggatgaa aattgccatc cattatacgc
    11521 ctgagattta acaatttttg cgaaactccc ctcacctttt ataacgctgt gccagtttat
    11581 tagcagcccg acttaacagc ggttccagcg ccacagccag caacattttc agcggtcggc
    11641 gagcaactga ttttatcgcc cagcccgcta cacccgccgg gccatagcgc agtgcggtaa
    11701 gaagtaccag cttgcctgct aatttgaaac caggctttac cttttgcccg gcctgttgcc
    11761 agcgagtatt cataactttc ctcacagttg acggaaacgg ctacgtaacg tgaaagtatc
    11821 ggaagtgaca taacgttcca tctcgcgtaa acgcgtttca cttgccgcca gttcacggtc
    11881 gacttcatcc agcaattcgc tgctggaagg tagctgctca ccaaaggcca tgttgtccgg
    11941 cattggatca agcgcaaatg acaaaatgat gtaagcaacc agggtaaaca gcgccagacc
    12001 gaagaaaatc gacagcacca ccaggatacg caccagtttt accggtacat caaaatagtt
    12061 ggcaatcccg gcgcagacgc cgcggaccat gccctgctgt ggaatacgcc ataatttttt
    12121 attgagatta atgcccgcca ttagcgatcc ctccagttcg gatgttctgc atcaagaata
    12181 gattccagcg cctgaatacg ttcgcgcatc cgttttgctt catcagccag ttgcgctaat
    12241 cgctgctgct cactttgcga caattcatcg cgaccagaac gattgctgta atgcagccat
    12301 aaccagatcg gtaaaacaaa cagcacaaaa atggttaacg gaatagccag aaatagcgcg
    12361 ctcataagta ctccttagac gatgagcggc ggcgcgttcg gacgccgcct ggatgttatt
    12421 attgattgtc ttgcttcatt ttggctttta attgtgccag ttgttcgctg attgcatcat
    12481 cggctttcag ttcggcaaac tgatcgtcca gcgatttttg tttaccgaag ctgtggcttt
    12541 ctgcttccgc ttccatctgg tcaattcgac gttcgaaaga ttcgaaacga gccattgctt
    12601 catccagttt gccactgtcc agctgacgac gcacatcgcg cgacgagttt gccgcctgat
    12661 gacgtaacat caatgcctgc tggcgagcgc gtgtttcgct caatttgttt tccagctcgc
    12721 caatctcttt cttcatgcgt gccagcgtat cgttcaccag cgtcacttca tgttccaggg
    12781 acttaatcag atcggtcagt ttctgttttt caattaacgc tgcacgtgcc agatcctctc
    12841 tctctttcag cagcgccagt tcggcttttt cctgccattc aacttcacgc gccgacgctt
    12901 gttcaatacg gcgagtcagc tgtttctttt ctgccaacgc acgcgccgaa gtagaacgta
    12961 cttcaaccag tgtatcttcc atctcctgga tcatcagacg caccagtttc tgtggatctt
    13021 ccgctttctc taacagagcg ttgatgttgg cattcacgat gtcggcaaag cgagaaaaaa
    13081 tacccataat gttgtcctct tgatttctgc gttcaggatt gtcctgctga actgttaata
    13141 caatttgcgt gccaattttt tatctttttg atttataaag atctgattga agaatcaaca
    13201 gcaacatgcc aggatgaatt agcgaattac actaacaagt ggcgaatttc atcatggccg
    13261 aatacaaaga taatttactt ggtgaggcga acagctttct cgaagtgctg gaacaggttt
    13321 cgcatctcgc accgctggac aaaccggtgc tcatcatcgg cgaacgcggc accggtaaag
    13381 agctgattgc cagccgcctg cattatctct cctcccgttg gcaagggccg tttatttccc
    13441 ttaactgcgc ggcgttaaat gaaaatctgc tggattccga actgtttggt cacgaagcgg
    13501 gggcgtttac cggtgcgcaa aaacgtcatc cagggagatt tgaacgtgcc gacggcggta
    13561 cgctatttct tgatgaactc gctacggcac cgatgatggt gcaggagaaa ttattgcgcg
    13621 tgattgagta cggtgaactg gagcgcgttg gcggcagcca gccattgcag gtgaatgtgc
    13681 ggttggtatg cgcgacgaat gccgatctcc cggcgatggt caatgaaggc acttttcgcg
    13741 ctgacctgct cgaccgactg gcttttgatg ttgtacaact gccaccactg cgcgagcgcg
    13801 aaagcgacat aatgttgatg gcagaacact ttgccatcca gatgtgtcgg gaaatcaagc
    13861 tgcctctgtt cccggggttt acggagcgcg ccagagaaac attgctgaat tatcgttggc
    13921 cgggaaatat tcgtgaattg aaaaacgcgg tggaacgttc agtgtatcgc cacggcacca
    13981 gcgattatcc gcttgatgac atcattattg atccctttaa acggcgtccg cctgaagacg
    14041 ctatcgccgt ttcagaaacc acctcgcttc cgacgctgcc gctggattta cgtgagtttc
    14101 aaatgcagca ggaaaaagag ttgctgcaac tcagtttgca aaaggggaaa tataaccaga
    14161 agcgcgcagc tgaattgctg gggttaacct accatcagtt ccgcgcgttg ttgaaaaaac
    14221 accagattta gcgaagttgg gcaatgcgat tcgccggatg cggcgtgaac gctttatccg
    14281 gcatacataa tcaacgtcgt ttgcaggcct gataagcgca gcgcatcaga cattattgcg
    14341 ttgggctatt aatcgctcag cgcatcctgc aaaattttca ttgccgcatc gaattgcgca
    14401 tccgggatgg tcagcggata caggaagcga atcacgttgc cgtatgcgcc acaggtcagc
    14461 aggagcagcc cctgcgccag cgcgcgttgc tggattttct gtgcaatcgc cgctgacggc
    14521 tcgcccgttt gcggatcgtt aaactctgcc gcaatcatcg accccaggcc gcgtaccgca
    14581 gcaatggccg gaacgctttc tttggcatca atcaacgtgt ttttgagacg ctggcccagt
    14641 tgattcgcgc gttcgcagag tgattctttg ttgataatat tgagcaccgc gtgcgcggca
    14701 gccaccgcca gcgggttccc ggcgtaggtg ccacccaacc cgcccggcgc gggcgcgtcc
    14761 ataatattcg cgttaccgac cacgcccgaa agcggcatcc cgcccgcgag gcttttcgcc
    14821 atcgtcatta agtccggctt atcggcgtaa tgatccatgg caaacagttt accggtacgc
    14881 gcaaagccgc tttgcacttc atcggcaatc atcacaatac cgtgctcgtc gcacaggcgg
    14941 cgaatggcgg caaccagctc ttttggcgca acgttgaaac cgccctcgcc ctgcaccggt
    15001 tcgaaaataa tggccgcgac ctgcttcgct tcgatgtctg atttaaacaa gcgttcgatg
    15061 gcgtcgaggg agtcctatgt tgaaatgccg tgtaaatctg acggataagg tacgtgatac
    15121 accgaaccag ggaacgggcc gaagccgatt ttgtacggcg caacttttcc ggtcagcgcc
    15181 atggtcatat atgtacgacc gtggaagcca ccgctaaacg caatcacgcc agggcgtccg
    15241 gtatgggcgc gagcaatttt caccgcgttt tccaccgctt ccgcaccggt agtgaagaac
    15301 gcggttttgg cctgcccgct caccggggca agggcgttga ttttctccgc cagggtgacg
    15361 tagctttcat acggcacaat ctgatacgcg gtgtgggtaa actgttgcag ttgctgctcc
    15421 accgccgcga ccagatcagg gtggcgatgt ccggtattca gcaccgcaat gcctgcggcg
    15481 aaatcgatgt actcgttgcc ctcaacatcc ttcagagtag cgttttcagc cgactgggcg
    15541 aagaagttac acatcacgcc aaccccgcgt ggggtagcag aaagacgacg ctgatggaat
    15601 tcattgttgc tcatgtttca caccttttga ctcttcactg ttaatcggaa attaaacgcc
    15661 cagacgatcg cgcaggctgt accaggctgc gcccatcgcg gtaaacggta ctcgcagcgt
    15721 gcgcccgccg gggaacgggt aatacggcag attggcgaag gcatcgaaac gttcggcgtc
    15781 gccgcgcagc agttcggcaa tcaaacgtcc ggcgagatga gtacaggtga cgccgtggcc
    15841 gctgtaaccc tgcatgtaat agatgttggt atcaaggcga ccaaactgcg gcatgcgcga
    15901 cagggtcagc aggaagttgc ccgtccagcg gtagtcgatt ttgacgccct tcagttgcgg
    15961 gaaggttttc agcagtttcg gcacaacaag acgctcaacg tcatccgggt cgcgcgcgcc
    16021 gtagaccacg ccgccgccgt acagcaaacg gttgtcggcg gtaagacggt agtaatcaag
    16081 cagataatta cagtcttcca cacagtagtt tttcgggatc agcgaacggg ctaaatcttc
    16141 cgacagccgt tcagtggtga tcacctgcgt gccgcacggc atgctgcgtt tcgccagttc
    16201 cggctctact ttatcgccca gatacgcatt cccggcgaca atcacatact tcgccgtcac
    16261 ctgacctttg gcagtgcgca ccaccgctgg cgtggtgtgc tggatctgcg tcacggcaga
    16321 aagctcatac acgcgcccgc cgttcaggcg gatggcatcg gcttcaccaa tggcgaggtt
    16381 tagcgggtga atatgcccac cgctgtgatc caacagtgcg ccagtgtagc gatcgctggc
    16441 gacctcgcgg cgaatggcgt tagcgtccag cagctccagc tgtttattgc cgtagcgttc
    16501 ccagttctct ttctgctctt cgagtgtggc gagctgttta tcattcatcg ccacaaacag
    16561 gccgccgggg cggtagtcgc agtcaatctg atagcgtttg atacgttcgc ggatgatctc
    16621 gccaccctcg aacatcatgc tgccgagcat acgggcggtg tccatgccgt agcttttttc
    16681 gatcacgtcg atgtcgcggc tgtaggagtt cacaagctgc ccgccattgc gcccgcttgc
    16741 gccaaagccg atgcgtgagg cttcgagaac cactacgtca aagcccgctt ccgccagatg
    16801 cagcgcggag gagagcccgg tatagccgcc gccaaccacg caaacgtcgc aggtgattga
    16861 ctcattcagt gtgtcgaatg gtgcatattt attcgcactg gcggcgtagt aactgctggt
    16921 atgttcggtc atttcaggcc tccaggctta tccagatggt tttcagttca gtgaattttt
    16981 caagggcatg cagggatttg tcgcgaccgt tgccgctctg cttatagccg ccaaacggca
    17041 cggtcatatc gccgtcgttg tagttattga cgaagacgga accggctttc aggcggcggc
    17101 tcatacggtg cgcgcgggag aggtcgcgcg tccataccgc cgcgccaagg ccgtactggc
    17161 tgtcgttggc aagctgtagc gcctggtctt ctgatgtgaa acgcgtgacc accagcaccg
    17221 gaccgaaaat ctcttcgcgg cttaaggacg cattcgggtc aacatccaca aagatggtcg
    17281 ggccgatggc ggcagccagc tcagcgttac gaccatccag cagcagttgc cctttgcttt
    17341 cgccttcacg aataaagcta tggaccgagt cggcgtgggc gcaatcgatt aaggtgccca
    17401 tggtggttac ggtatcaagt ggatggcccg gctgccagtt ttgcgcctgc tgttttaaca
    17461 gggctaagaa ttcatcggcg atgctctctt ccagtaacaa acgcgttccg gcgatgcaca
    17521 cctggccctg gttgtagaag atgcctgcgg cggtggcgct tgccgctttt tgcagatccg
    17581 gacaatcggc gaaaacgatg ttggcgcttt tgccgcccgc ttccagccag acgcgtttca
    17641 tgttgctatc acccgcgtct ttcagcagtt gtttacccgt acgcgtggag ccggtaaagg
    17701 cgatggcatc gatatcgtta tgacgcgaaa gcgcctgccc ggcttcatga ccataaccgc
    17761 tcactacgtt cagcacgcca tccggcagcc ccgcttcttc ttttgccagc ccggcgagac
    17821 gaatcgcact gagtggtgat ttttcagacg gttttagaat cacgctgttt cccgccgcca
    17881 gcgccgggcc gagtttccag caagtcagca gcagcgggaa gttccacggc acgatggcgg
    17941 caatcacgcc gaccggttca cgcacgatca tcgccagctc atggctactg gtggtcgcca
    18001 cttcgccata cactttgtcg atcgcttcgg cgtaccagcg aatggcgcgc gccgcgccgg
    18061 gaatatcatc acgcagactg tgacgaatcg gtttgccggt gtcgagagtt tccagcagtg
    18121 ccagctcttc ggcgtgggct tccattaaat cggcgagttt attcagtacc gctttacgtt
    18181 tagccggaga agagagtgac cagtcgccgc gttcaaatac gccgcgtgct gcgctcaccg
    18241 catggtcgat atcgacgctc ttgccgcggg caattttcgc cagcggtgcc taggtgaccg
    18301 gatcaacggt ttcaaaggtt tcattttccg ccgcagcagt atattcaccg ttaataaata
    18361 agcggttttc aatggcgaga cttaacgctt tatcctgcca gtaagccaga tgatgaaaat
    18421 tcattatgac tcctgtttca cgtctatcag atatatgcag tttgtgaacg tcagggcgca
    18481 gagatccccg ggccgcatgc ccgtttattt ctggcgacat tctttatgga ttgccgggcg
    18541 cattgcgctt acccggctta cagaacaaaa agattaaaac gtggtgggcg tatgggcgct
    18601 gataattcgg caaatacctg ccgacgtatt actgaaactg tgcgggatgc cggtattaat
    18661 ggcataactt tgccccgcga cgaggtggta atcctgacca ttaatcgtca gaacaatttc
    18721 accttccagt actgtgccta tttcctcacc ctgatgctta attctttccc cagtggttgt
    18781 gcccggctgg tacgtttcaa agatcatcgc cagcgtgcga ttcgggttac cgttatgaac
    18841 cagcttcatt gacacaccct gactgcccat ctcaattaag tcgtcctgat taatgacgac
    18901 ctgcggctca tcaggttttt ccggctcgga aaagaattcc gagagtgaca gaccatacac
    18961 cttcagcagc ttttgcagcg tactgatggc agggctgact ttatcttgtt ctatcgtact
    19021 gatagcactg tgagtcagcc cggagagttc ggcggcacga cgttgtgaaa gcccctgctg
    19081 ctggcggatt tccgacaagc gttttcctgg cgccagtccc tcatcactca tatttgcatt
    19141 tccttaaact gtagtggtca gagtcgctgt ttttcagcga tatggtgctg acaagcggtg
    19201 ataaagccct cgaacaatat acgcgaaagc gcgtactcgc tactgttcca ttccgggtgc
    19261 cactgtacgc ccagcgcaaa aggatgattg atgacgctaa ccgcctcaac taagccatcc
    19321 ggcgagcgag cttcaacacg caaccgtggg ctaacgacct tcgccccttg tccatgtaga
    19381 gagtttaccc aaaagttgct acattcaggt aacaacgcag acagtaatcc cccctcttca
    19441 acctgaactt catgcgacgg tgcatattgc tgttccaccg gcagttcggg atcttcccga
    19501 tgttccagca attcaggctg ttcgcacagc ttgcgatgca acgaaccacc ggttgccacc
    19561 accagttctt gtaaaccccg gcagatggcg aaaatgggga tgcgcctttc gagtgcggca
    19621 ttaattatcg ccatgctcag aagatcacgc ccgggatcgg cgtcaggctc atcgccgttt
    19681 tcaccatata ggtgcggctg cacattgctg ggactaccag gaagataaat gccatcgagt
    19741 ttcggcaaaa gttgttcaag taacgacggt tccgccagcg catgtggtag cgcaattggc
    19801 aagccgcctg cgtggatgat ggcattcagg tacttttctt gcagagtctg ggtcgcatga
    19861 cccttaagcc tgttcctgca cattacgaca ccgataaccg gattgttcat tatattttcc
    19921 atgctcaatc tcacaaagtg gactaaatta tcgccattac tgcgctttaa atgaccttta
    19981 tgttcaatat tttttcaatc tagcagtggg ttttcatttt tgcaaactca atttaacatt
    20041 tgacaaacat ttagtttgca tacagattcg aatggtggtc attatatttt acgctttgat
    20101 aacgagcgga aaacaaacca aaggcgaaga atcatggaaa ccaatatcgt tgaagtagag
    20161 aactttgttc agcagtcaga agagaggcgg ggtagcgcct ttacgcagga agtgaaacgc
    20221 tacctggagc gctacccgaa tacgcaatat gttgatgttt tacttactga tttgaatggt
    20281 tgcttccgtg gaaaacgtat ccctgtttca agcctgaaga agctcgagaa agggtgttat
    20341 ttcccggcct cggtatttgc aatggatatt ctgggcaacg tagtagaaga agcaggtctg
    20401 ggtcaggaaa tgggcgagcc ggatcgtacc tgtgttcctg ttctcggttc cttaactcct
    20461 tctgccgccg atccagagtt tatcggtcag atgctcctga ccatggtcga tgaagatggc
    20521 gctccctttg acgttgagcc gcggaacgtt cttaaccgcc tgtggcagca gctgcgccag
    20581 cgcgacgctg aagggaatct gcaaccgccc tgcgcgcctg gcaccgatga ccgcaataca
    20641 caaagccagg tttactccgt tgataacctc aaccacttcg ccgacgtgct caatgatatt
    20701 gatgaactgg cgcagttgca gctgattccg gcagatggcg cggtcgctga ggcctcgccg
    20761 ggtcagtttg aaatcaacct ttaccatact gataacgtgc tggaagcctg cgatgatgcg
    20821 ctggcactaa aacgcctggt gcgtctgatg gcaggaaagc ataagatgca cgccactttt
    20881 atggcgaagc cgtatgaaga gcacgcgggc agcgggatgc atatccatat cagtatgcaa
    20941 aataatcgtg gcgagaatgt gctttctgac acagaaggcg aagattcgcc gctgctgaaa
    21001 aagatgctcg ccgggatgat tgacctgatg ccgtcgtcga tggcgttgct ggcaccaaac
    21061 gtgaactcgt atcgccgctt ccagccggga atgtatgtgc cgacgcaggc gtcgtggggc
    21121 cataacaacc gcaccgtcgc cctgcgtatt ccgtgcggcg accgtcataa tcaccgcgtg
    21181 gaatatcgcg tggcgggtgc cgatgccaac ccatatctgg tgatggcagc gatttttgcc
    21241 ggtattttgc atggccttga taacgagctg ccgttgcagg aagaagtcga aggcaacggg
    21301 ctggaacagg aaggcttacc cttcccgatt cgccagagcg atgccctggg tgagtttatc
    21361 gagaatgatc acctgcgccg ctatttaggc aaacggtttt gccatgtgta tcacgcctgt
    21421 aaaaatgatg agctgttgca gtttgagcgc ctcatcacag aaacagaaat tgagtggatg
    21481 ttgaaaaacg cgtaattccc cttgcggggt tgtttcatcg gcctgagcgg agccaggcct
    21541 gacatttgct ggtaatccag acatttcccg gatgtcgcgt tatggagcgc aggcggcaac
    21601 gggcttccgt taacgattaa gactgtgacg aggaaatgag atgatgccga tgattaaatc
    21661 cccgcatggc gagggaggct gcgtatgcgc ccctcctgct actgactgga ctccgccccc
    21721 tttgcttccc ttgctaaaca ggtttgattt caggtcgaca cgaccgcaaa ccttattacg
    21781 caggggaggc agcaattatg gctattaatt caccactgaa tattgctgcg caacccggca
    21841 aaacccgtct gcgaaaatca ctgaaattgt ggcaggtggt aatgatgggt ctggcctatc
    21901 tcacgccgat gaccgtattt gatacttttg gtattgtctc cggcattagc gacggtcacg
    21961 ttccggcgtc ctatttgctg gcgctggcgg gcgtgctgtt taccgctatc agctacggca
    22021 aactggttcg ccagtttccg gaggccggtt cggcctatac ctacgcgcaa aagtcgatta
    22081 acccgcacgt cggatttatg gtcggctggt catcactgct ggattatctc tttttgccaa
    22141 tgatcaacgt tttgttggcg aaaatctatc tctccgccct cttcccggaa gtgccgccgt
    22201 gggtgtgggt ggtaaccttc gtcgccattt taaccgccgc gaatctgaag agcgtcaacc
    22261 tggtcgctaa cttcaatacc ctgtttgtac tggtgcaaat ctccatcatg gtggtgttta
    22321 tcttcctggt ggttcaggga ctgcataaag gagaaggcgt tggcaccgtc tggtcacttc
    22381 agccgtttat cagcgagaac gcgcacctga tcccgattat taccggtgcg acgattgtct
    22441 gtttctcgtt cctcggtttc gatgcggtga ccacgctttc ggaagagacg ccggacgccg
    22501 cacgcgtgat cccgaaagcc atcttcctga cggcggtata cggcggcgtt atctttatcg
    22561 cggcgtcgtt ctttatgcag ctgttctttc ccgatatcag ccgctttaaa gacccggacg
    22621 ccgcactgcc tgaaattgcg ctgtacgtcg gcggcaagct ctttcagtcg attttcctct
    22681 gcaccacgtt tgtgaacaca ttagcgtctg gcctggcttc acatgccagc gtgtcgcgtc
    22741 tgctgtatgt gatggggcgt gacaatgtgt ttccggagcg cgtgtttggc tatgtgcacc
    22801 caaaatggcg gactccggca ctcaacgtca ttatggtcgg gattgtcgcg ctgtcggcgc
    22861 tgttctttga tttagtcacc gcgacagcac tgattaactt cggtgctctg gtggcgttta
    22921 ccttcgtgaa tctgtcggtg tttaatcatt tctggcggcg taaaggaatg aataaaagct
    22981 ggaaggatca cttccactat ttgctgatgc cgctggttgg cgcgctgacg gtgggtgtgc
    23041 tgtgggttaa cctagagtca acgtcactga cactcggtct ggtatgggct tcgttaggcg
    23101 gcgcatattt gtggtatctg atccgccgct atcgcaaagt gccgctgtac gaaggtgaca
    23161 gaacgccggt gagcgaaacg taatagccgg atgcgcggcg cattatcctg cctgagggta
    23221 acgcgccgcg ccattaacta taaaaagatg tataacccgg atcacgagtg ctatcatccg
    23281 cccttatgct tagcgcatta accccagggg caaataatga accacgatat cccgctaaaa
    23341 tattttgata ttgccgatga gtacgcgacc gaatgcgcag agcctgtcgc agaggctgaa
    23401 cgcacaccgc tggcccacta cttccagtta ctgctcaccc gtttgatgaa caatgaagag
    23461 atcagcgaag aagcccaaca tgaaatggct gctgaagcgg ggattaatcc cgtacgcatt
    23521 gatgagatcg cagagttttt gaatcaatgg ggtaatgagt aattatttaa ttgcagacga
    23581 tcggtcagta tcgttgtccg gataaagcgt tcacgccgca tccggctaac aatgacacgc
    23641 actgcagcgt gctttaatgg gtttctcacg atcgtaccgc tctgctcctc gctaagttgt
    23701 tctgtgagtt atagttatag agaaatcacg ggtacgaatg ttgaacagat ggcgacgcca
    23761 cctgttcaac gaccaaccac acggccctca ttacgacccc tgaacacaaa cggtaatggc
    23821 aaatgtcatt aaatattagt aagatagcct ctttacataa aatcccttaa tattatgcgc
    23881 caggtattat cgtctctttt ggtgattgct ggacttgtga gtggtcaggc aatcgccgcg
    23941 cctgaatccc ccccgcatgc tgatatccgc gacagcggtt ttgtctattg cgtcagcggg
    24001 caagtcaaca cctttaaccc atccaaagcg agcagtgggt taattgtcga tacccttgcc
    24061 gcccagtttt atgatcgact gctggatgtc gatccctata cctatcgcct gatgccggaa
    24121 cttgccgaaa gctgggaagt actcgacaac ggcgcgacct atcgcttcca cctgcgtcgc
    24181 gatgttccgt ttcaaaaaac cgactggttt actcccactc gtaaaatgaa tgccgacgat
    24241 gtggtgttta ccttccagcg aatttttgac cgcaacaacc cgtggcataa cgtcaacggc
    24301 agcaacttcc cctacttcga cagcctgcaa tttgccgata acgtgaaaag cgtccgcaaa
    24361 ctggataatc ataccgttga gttccgactc gcccagccgg atgcttcttt tttgtggcac
    24421 ctcgcaaccc attatgcttc ggtcatgtcg gcagaatatg cccggaagtt agagaaagaa
    24481 gatcgccagg agcaactcga ccgtcaaccg gtcggcaccg gtccgtatca gttgtcggaa
    24541 taccgcgccg ggcaatttat tcgcctacaa cgtcatgatg acttctggcg cggtaaaccg
    24601 ttaatgccgc aggtagtggt ggatttaggc tccggcggca ccggacgtct gtcgaaactc
    24661 ctgaccgggg aatgcgacgt tctggcctgg cctgctgcca gccagctatc cattttgcgt
    24721 gacgacccgc gcttgcgttt aacgctgcgt cctgggatga acgtcgccta tctggcattt
    24781 aacaccgcca aaccgccgct aaataatccc gctgtccgcc atgcactggc actggcgatt
    24841 aataaccagc gcctgatgca atccatctat tatggtacgg ctgaaacggc ggcctctatt
    24901 ttaccgcgcg cctcgtgggc ctatgacaac gaggctaaaa ttactgaata caatccggcg
    24961 aaatcgcgcg aacagttgaa ggcgttgggg ctggaaaact taacgctgaa actgtgggtg
    25021 cccacacgtt cgcaggcgtg gaaccccagt ccactgaaaa ctgccgaact gattcaggcg
    25081 gatatggcgc aggttggcgt aaaagtggtg attgtgccgg tagaaggtcg ctttcaggag
    25141 gcgcggttga tggatatgag ccatgatctg acattatccg gttgggcgac ggacagtaac
    25201 gacccggaca gtttcttccg tcctttactg agctgcgcgg caattcattc acagaccaac
    25261 ctcgcccact ggtgcgatcc gaaattcgac agcgtgttgc gtaaggcgct ctcctcgcag
    25321 cagctggcgg cgcgtattga agcctatgac gaagcgcaga gtattctggc gcaggaatta
    25381 cctattctgc cgctggcgtc gtcattgcgt ttgcaggcct atcggtacga tatcaaaggt
    25441 ctggtactta gcccgtttgg taacgcctcc tttgctgggg tgtatcgcga gaaacaggat
    25501 gaggtgaaaa aaccatgatt atcttcacct tacgccgcat tttgttattg attgtcacct
    25561 tgttcctgct gaccttcgtt ggcttcagct tgagctattt cacccctcat gcgccgttac
    25621 aaggcgcgtc actgtggaat gcctgggtgt tctggtttaa cggcctgatc cactgggatt
    25681 ttggtgtttc cagcattaac ggtcaaccga ttgccgaaca gttaaaagag gtcttcccgg
    25741 cgacgatgga actgtgcatc ctcgccttcg gttttgcgct gattgttggc atccccgtgg
    25801 ggatgattgc cgggattacg cgccataagt ggcaggataa cttgatcaac gccatcgcct
    25861 tactgggctt ttcaatcccg gtgttctggc tggcgcttct gttgacgctg ttttgttcac
    25921 tcacgctagg ttggttgccc gtttcagggc gtttcgatct gctctacgaa gtgaaaccga
    25981 ttaccggttt tgcgttgatt gatgcctggc tttcggactc accatggcgg gatgaaatga
    26041 tcatgagcgc aatccgccat atgatattgc ccgtgatcac cctgtcggtt gcgccaacaa
    26101 ctgaagtgat ccgcctgatg cgtatcagca ctatcgaagt gtatgaccag aactatgtaa
    26161 aagcggcggc gacccgcggt ttgtcgcgct ttactatttt gcgtcgccac gttctgcata
    26221 acgcgttacc gccggttatt cctcgcctgg gcttacagtt ttccaccatg ctgacgctgg
    26281 cgatgatcac cgaaatggtt tttagctggc cgggtttagg gcgctggtta attaacgcca
    26341 tccgccagca ggattacgca gccatttccg ccggagtgat ggtgtgtggc tcactggtca
    26401 ttattgttaa cgtgatttct gatattttgg gtgccatggc taaccctctg aaacataagg
    26461 aatggtatgc cttacgatag cgtatacagc gaaaagcgcc cgccgggcac gctgcgtacc
    26521 gcctggcgca aattttatag tgacgcctct gcgatggtcg gcctgtacgg ctgcgcgggg
    26581 ctggctgtac tgtgtatttt tggcggctgg tttgcgcctt acggcatcga ccagcaattt
    26641 ctcggttatc aattattgcc gccatcatgg tcgcgctatg gcgaagtttc tttcttcctg
    26701 gggactgacg acctcggacg cgatgtttta agccgtttac tgagcggagc tgcgccaacc
    26761 gtgggtggcg catttgtggt gacgcttgcc gcgacgatct gtggcctggt actgggcacc
    26821 ttcgccgggg cgacacacgg cctccgctca gcggtgctta accatattct ggatacctta
    26881 ctggcgatcc cttcgctgct gttggcaatt atcgttgtag cgtttgccgg accgagtttg
    26941 tcccacgcta tgtttgctgt ctggctggca ttgctgccgc gtatggtacg ttcgatttac
    27001 agcatggtgc atgacgaact ggaaaaagag tacgttatcg ccgcccgtct ggatggcgca
    27061 tcaacgctga atattctctg gtttgctgtg atgccaaaca tcaccgccgg gctggtcacc
    27121 gagatcaccc gcgcactgtc gatggcaatt ctcgatatcg ccgcgctggg ctttctcgat
    27181 ctcggcgcac aactcccctc gcctgaatgg ggagcgatgc tcggtgatgc gctggaactg
    27241 atttatgtcg ccccgtggac tgtcatgctg ccaggtgcgg caattatgat tagcgtgttg
    27301 ttggttaacc tccttggcga tggcgttcgt cgtgcgatta ttgcgggggt ggaataatgc
    27361 cattactcga tattcgtaac ctgaccattg aatttaaaac cggtgatgag tgggttaaag
    27421 ccgtcgaccg ggtaagtatg accttaaccg aaggtgaaat ccgcggtctt gttggtgaat
    27481 ccggttccgg caagagtttg attgcgaaag cgatttgtgg ggtgaataaa gataactggc
    27541 gtgttactgc tgaccgtatg cgttttgatg atatcgattt gctgcgtctc tccgcacgcg
    27601 aacggcgcaa actggttggc cataacgtgt cgatgatttt ccaggaaccg cagtcgtgtc
    27661 ttgacccttc agaacgtgtg ggtcgccagt tgatgcaaaa catcccagcc tggacctata
    27721 aaggccgttg gtggcagcgt tttggctggc gcaaacgccg tgccattgaa ctgctgcacc
    27781 gcgtggggat taaagatcac aaagatgcga tgcgcagttt tccctatgag ttgaccgaag
    27841 gtgaatgtca gaaagtgatg atagccattg cactggcgaa tcaaccgcgt ctgctgattg
    27901 ctgacgaacc gaccaactca atggagccaa caacccaggc gcaaatcttt cgcctgctga
    27961 cgcgtctcaa ccaaaacagc aataccacta ttttgcttat cagccatgac ttacaaatgc
    28021 ttagccaatg ggcggataaa attaacgtgc tttactgcgg tcaaacagtg gaaaccgcgc
    28081 cgagtaagga gttggtgacg atgccacatc atccttatac ccaggcgctg atccgcgcga
    28141 taccagactt cggcagcgcg atgccgcata aaagtcgcct caatacgctg cccggcgcta
    28201 tcccactgct ggaacagtta ccgattggtt gtcgtctggg gccacgttgc ccgtatgcac
    28261 aacgagaatg cattgtgacg ccacgtttga cgggggcgaa aaatcatctc tatgcctgtc
    28321 atttcccgct gaacatggag aaagagtgag atgatcgaaa cgctgcttga agtgcgtaat
    28381 ttaagtaaaa cgttccgcta ccggaccggc tggtttcgtc gtcagaccgt agaagcggta
    28441 aaacccttga gctttacgct acgtgaaggc cagacactgg cgattattgg cgagaatggt
    28501 tcgggtaaat ccacgctggc aaaaatgctg gcgggaatga tagagcctac cagcggcgag
    28561 ttattgattg acgatcatcc actgcatttt ggcgattatt ccttccgcag tcagcgtatt
    28621 cgcatgattt ttcaggatcc ttcgacctcg ttgaatcccc gtcaacgtat ctcgcaaatt
    28681 ctggattttc cactgcgcct gaacaccgac ctggaaccgg aacagcgtcg caaacagatt
    28741 attgaaacga tgcgtatggt tgggctgcta ccggatcacg tcagttacta tccgcatatg
    28801 ttggcacccg gacaaaaaca gcgtctgggt ctggcgcgcg cgctaatatt gcgcccaaaa
    28861 gtcattattg ccgatgaagc cctcgcttca ctggatatgt cgatgcgttc gcagttgatt
    28921 aatctgatgc tggagttaca ggaaaaacag ggcatttcgt atatttatgt tacccagcat
    28981 atcggaatga tgaagcacat tagcgaccag gtgctggtga tgcatcaggg cgaggttgtc
    29041 gagcgaggca gcaccgcgga tgtgctggca tcgccgctgc atgaactcac caaacggctg
    29101 attgccgggc actttggcga agcattgaca gcggatgcgt ggcgtaaaga tcgctaattt
    29161 tctgaatcct ccttccagat tatccacctc attctgacaa gagattaaat cttgtcagaa
    29221 tgaggtgatg gataaaataa aatcaaatgc gcgtgattta agacgcaatc tcactttgca
    29281 ggaacgaaag ctctggcgat atcttcgcag ccgacgtttt ggtgatttca aatttcgccg
    29341 tcaacatccg gtggggagct acattctcga ttttgcttgc tgctcggcgc gcgtagtcgt
    29401 tgagctggat ggtgggcagc atgatttggc agttgcctat gataccaggc gcactagctg
    29461 gcttgagtcg cagggctgga ccgtgctgcg tttctggaat aacgagattg attgtaatga
    29521 ggaggcggtg ccggagatta ttctgcagga actgaaccgc cgatcaccct caccctgaaa
    29581 gggcgagggg gccgaccgag ccgaatagct gttgtggtga aaacatggag acagtgctgg
    29641 agaatattcg gcaaggtctg aaccatccca gccatcgcca taaaagggtt aggggctgta
    29701 tgagcctgtt tgttgctggg gtaacaatat ttgcacaata tggtcccctc gcccctctgg
    29761 ggagagggtt agggtaaggg gaaaagcgcc caccctgccg cagcctgctc cggtcggacc
    29821 tggcaactat agctactcac agccaggttg attataataa ccgtttatct gttcgtactg
    29881 tttactaaaa cgacgaatcg cctgattttc aggcacaaca agcatcaaca ataaggatta
    29941 aagctatggg ttttctttcc ggtaagcgca ttctggtaac cggtgttgcc agcaaactat
    30001 ccatcgccta cggtatcgct caggcgatgc accgcgaagg agctgaactg gcattcacct
    30061 accagaacga caaactgaaa ggccgcgtag aagaatttgc cgctcaattg ggttctgaca
    30121 tcgttctgca gtgcgatgtt gcagaagatg ccagcatcga caccatgttc gctgaactgg
    30181 ggaaagtttg gccgaaattt gacggtttcg tacactctat tggttttgca cctggcgatc
    30241 agctggatgg tgactatgtt aacgccgtta cccgtgaagg cttcaaaatt gcccacgaca
    30301 tcagctccta cagcttcgtt gcaatggcaa aagcttgccg ctccatgctg aatccgggtt
    30361 ctgccctgct gaccctttcc taccttggcg ctgagcgcgc tatcccgaac tacaacgtta
    30421 tgggtctggc aaaagcgtct ctggaagcga acgtgcgcta tatggcgaac gcgatgggtc
    30481 cggaaggtgt gcgtgttaac gccatctctg ctggtccgat ccgtactctg gcggcttccg
    30541 gtatcaaaga cttccgcaaa atgctggctc attgcgaagc cgttaccccg attcgccgta
    30601 ccgttactat tgaagatgtg ggtaactccg cggcattcct gtgctccgat ctctctgccg
    30661 gtatctccgg tgaagtggtc cacgttgacg gcggtttcag catcgctgca atgaacgaac
    30721 tcgaattgaa ataatcattc tgttggtaaa tattgggcgg cgttctgccg cccgttatct
    30781 ctgttatacc tttctgatat ttgttatcgc cgatccgtct ttctcccctt cccgccttgc
    30841 gtcaggataa cgatttcctt tacgaccaag gagcgcccat ggaacaacgc cacatcaccg
    30901 gcaaaagcca ctggtatcat gaaacgcaat ccagtactgc ggagtatgac gttctgcctc
    30961 tggtcccgga agccgcaaag gtcagcgatc cctttctgct cgacgtgatc cttgatgaag
    31021 aaacgctggc tcccttcctt tcatggctgg tccctgcgcg cgttcttgca gtggaattgt
    31081 tccctgacca gcttaccgtg acccgttcac agaccttcac cgcttatgaa cgcttgtcta
    31141 cggccctgac ggttgctcag gtttgcggcg tccagcggtt atgtaactac tattcggcgc
    31201 gacttacgcc gctccccggg cctgattcct ccagggaaag caatcatagg ttggcacaaa
    31261 tcacgcaata tgcccgccaa ctggctagct cgccttctat tatcgacaac cgatcgcgcc
    31321 agcatctgaa tgacgtcggt cttactgcct gggactgtgt gatcattaac caaatcattg
    31381 gttttattgg ctttcaggcg cggaccattg cgacatttca ggcttatctc gggcacccgg
    31441 tacgctggtt acccggtctg gaaatacaaa actacgccga cgcgtcactg tttgctgatg
    31501 aatcaatacg ctggcgaagc agctatgagg tggaaaaact acctgaagag tacacaaaaa
    31561 gttcaacagc agaactttgc caactggccg aaacactctc tctccaccct atttcacttt
    31621 cccttctcga aaggttgtta aacagtacac gggttaatac acagccggat aataagcttg
    31681 cggcgttgtt atgcgcgcgg ataaatggca gtcctgcttg ttttgccgcc tgtatggatt
    31741 catcaaatga atataaaaaa tcagccccct tctgcgcaag ggcgaaaatg aaattaacca
    31801 atgggctgac cgtcattctg ttgagcacgc taccgttcag gcgatacaat ggctgacccg
    31861 agcacccgat cgctttagcg ccgcccagtt cagcccttta ctcggacacg aaaaatcatc
    31921 aacgcagatt attaatctgc tggtatggag cgggctgtgt ggctggataa atcgcttaaa
    31981 aatcgcgttg ggtgagacat attaaccttg ccgcgtcaga cagattcgcg taaaactgtc
    32041 agccgctcta atggccacca aaatagacaa ttatgtttca ggacaacccg ctgctagcgc
    32101 agcttaaaca gcaactgcat tcccagacgc cacgcgctga aggggtggta aaagccacag
    32161 aaaaaggctt tggcttcctg gaagtcgacg cgaaaaaaag ttatttcatt ccgccgccgc
    32221 agatgaaaaa agtcatgcat ggcgaccgaa ttatcgcggt gatccacagt gaaaaagaac
    32281 gtgaatccgc agagccagaa gaactggttg aaccgttcct gactcgtttc gtgggtaaag
    32341 ttcagggcaa aaatgaccgt ctggccatcg ttcctgatca tccactctta aaagacgcca
    32401 ttccttgccg cgcagcccgt ggcctgaacc acgagtttaa agaaggcgac tgggcggttg
    32461 ccgaaatgcg ccgtcatccg ctgaaaggcg atcgttcttt ctatgctgaa ctgacacaat
    32521 acatcacttt tggtgacgac cactttgtac cgtggtgggt tacccttgcg cgccataatc
    32581 tggaaaaaga agcaccagac ggcgtcgcta ccgaaatgct cgatgaaggt ctggttcgtg
    32641 aagatctgac cgcgctggat tttgtcacca tcgacagtgc cagcacagaa gatatggatg
    32701 acgccctttt cgctaaggcg ttgccggatg acaaacttca gctgattgtg gcgattgccg
    32761 atccaaccgc gtggattgct gaaggcagca agctggacaa agccgcgaaa attcgcgcat
    32821 tcaccaacta tctgcctggc ttcaacatcc ctatgcttcc tcgcgagctt tctgacgatc
    32881 tctgctcact gcgcgccaat gaagtccgcc cggtactggc atgccgcatg acgctctccg
    32941 ctgatggcac cattgaagat aatatcgaat tctttgccgc caccatcgaa tccaaagcga
    33001 agctggtgta tgaccaggtt tctgactggc tggaaaatac cggtgactgg cagcctgaaa
    33061 gtgaagcaat tgccgaacaa gtccgtttgc tagcgcaaat ttgccaacgc cgcggcgagt
    33121 ggcgtcataa ccacgcactg gtgtttaaag atcgcccgga ttaccgcttt attctcggtg
    33181 aaaaaggtga agtgctggat atcgtcgccg agcctcgtcg cattgccaac cgtatcgtcg
    33241 aagaagcgat gattgccgct aacatttgtg cggcccgcgt tctgcgcgat aagctcggtt
    33301 ttggcatcta taacgtgcat atgggctttg atccggcgaa tgccgacgcg ctggcagcgt
    33361 tgctgaaaac gcacggtctg catgtcgatg ccgaagaagt gctcacgctg gacggtttct
    33421 gcaaactgcg tcgtgaactg gatgcgcaac caactggttt cctcgacagc cgcattcgtc
    33481 gcttccagtc atttgctgaa attagcactg aacccggtcc tcactttggc ctcggtctgg
    33541 aagcatacgc cacctggact tcgccgatcc gtaaatatgg cgacatgatc aaccaccgtc
    33601 tgctgaaagc ggttatcaaa ggcgaaactg cgacgcgtcc gcaggatgaa atcactgtcc
    33661 aaatggccga acgtcgccgt ctcaaccgga tggcagaacg tgatgttggc gactggttat
    33721 acgcacgctt cctgaaagat aaagccggga ccggcacccg tttcgcggcg gaaattgtcg
    33781 atatcagccg tggcggcatg cgtgtgcgtt tggttgataa cggcgctatc gcctttattc
    33841 cggcaccttt cttacacgct gtgcgcgatg aactggtttg cagccaggaa aacggcactg
    33901 tacaaattaa aggtgaaacg gtttacaaag taactgacgt tattgacgtc accattgccg
    33961 aagtccgcat ggaaacccgc ggcattattg cgcgcccggt cgcgtaatct cctttcacgg
    34021 cccattcctc atggatgggc cgtttatttc cccgctctac cttcgttata tccaggcaga
    34081 ttttttaact gactttcgtt tgaaaactgg cgtttttcca aaactggatt acaaataaat
    34141 atataaaaaa acaatgcgtt tagccgccgt taatttcatg taagtcatgt tgttcctttt
    34201 atttaaccgc ggactccgca gttaaccgga ggatatgcat catgaaaacc gttagggagt
    34261 ccacaacgtt gtacaacttt ctcggatcgc acaatccata ctggcggttg acggaaagca
    34321 gcgatgtttt gcgctttttt accaccgaaa ccacagaacc tgatcgtact ttgcagttat
    34381 ctgccgaaca ggctgctcgc atcagggaaa tgacggtcat cacctccagc ctgatgatga
    34441 gtctgaccgt cgatgagagc gatctttctg tgcatctggt aggacgaaaa atcaataaac
    34501 gggaatgggc tggcaacgcg tctgcatggc atgacacacc ggcagttgct cgtgatttat
    34561 cacacgggct ttcctttgct gagcaggtag tttctgaagc acattccgca atagtgattc
    34621 tcgacagccg ggggaatatc caacgcttca atcggttatg tgaagattac actgggttga
    34681 aagaacacga cgtcattggc caaagcgtgt ttaaactgtt tatgagccgt cgtgaagctg
    34741 cggcatccag gcgcaataac cgtgtatttt ttcgaagcgg caatgcatat gaagtcgaac
    34801 tgtggatacc aacacgtaaa ggccagcggc tgtttctgtt tcgcaataaa ataaatttgt
    34861 ccacagcggc agtggcaaaa acgagatttt tttaatctgt tccggtaccg acattaccga
    34921 agagcgccgc gctcaggagc gactgcgtat tctggcaaat accgacagta tcaccggact
    34981 gccgaatcgt aacgcaatgc aggagttaat cgatcacgct attaatcagg ctgataacaa
    35041 taaagttggg gttgtgtatc ttgatttgga taattttaaa aaggtcaacg acgcctatgg
    35101 gcatttgttt ggtgaccagt tattacgcga cgtgtcattg gctattttaa gctgtctcga
    35161 acatgaccag gtgttggcgc gtccaggtgg cgatgagttt ctggtactgg catccaatac
    35221 ctcacaaagc gcgctggaag caatggcatc acgaattttg acccgcttac gcctcccctt
    35281 tcgtattggt ttaattgaag tttataccag ctgttcagta ggtatttcgc tctctcccga
    35341 acatggttcg gacagcgcga ctattattcg tcacgccgac acagcaatgt acacagcgaa
    35401 ggaaggcgga cgaggacaat tttgcgtttt tactccagaa atgaatcagc gggtatttga
    35461 atatctctgg ctggatacca acttgcgtaa agcactggaa aacgatcagt tggttattca
    35521 ctatcaaccg aaaatcacct ggcgtggcga agtgcgcagt ctggaagcac tagtacgttg
    35581 gcagtcacct gaacgtgggt tgattccacc gttggacttc atttcctacg ccgaagagtc
    35641 agggctaatt gtgcctttag gccgttgggt gattctcgat gtcgtacgcc aggtggcaaa
    35701 gtggcgggat aaaggcataa acctgcgagt ggcggtaaat atttctgcac gtcagctcgc
    35761 cgatcaaacc attttcaccg ccctgaaaca ggttctccag gaactcaatt ttgaatactg
    35821 ccctatagat gttgaactga cagagagttg tctgattgag aatgatgaac tggcactgtc
    35881 tgttattcaa caatttagcc aactaggtgc gcaagtgcat ctggatgatt ttggtaccgg
    35941 ctactcttca ctttcgcaac tggcgcgctt tccgatcgat gccatcaaac ttgaccaggt
    36001 ttttgttcga gatattcaca aacaacctgt ctcgcagtca ctggtccggg cgatcgtcgc
    36061 tgtggcccag gcattgaatc ttcaggtgat cgccgaaggt gtagagagtg caaaggaaga
    36121 tgctttttta accaagaacg ggatcaatga gcggcaagga tttttgtttg ccaaaccgat
    36181 gcccgccgtc gccttcgaac gctggtataa acgctatctg aagcgcgcat aagtagatga
    36241 atatatatac ttagcgctat atcccgtcat tatatatcgt attcctggat gataattagc
    36301 cgttgctgtt aaacacttct cttttagcgg caatttttgc attattcatt ttccactttt
    36361 gctataagga tgtccttgat gtctgaattt gatgcccagc gcgttgccga acgtattgat
    36421 attgtgttag atatccttgt ggcaggcgat taccactctg cgatccataa tctcgagata
    36481 cttaaagcag aactcttgcg ccaggttgct gaatctacgc cagacatccc gaaggcaccg
    36541 tgggagattt aacaatcgtg ccttcaggtt ttcctatttg aggatgcccg gcgcgtcagt
    36601 atttttttat ttagtattat aacgttataa gaattacagc gatgaattcc cgacaacaaa
    36661 ctattctaca gatggtcatt gaccagggtc aggttagcgt aaccgatctg gcaaaagcca
    36721 ctggagtttc tgaagttacc attcgccagg atctcaacac cctcgaaaaa ctgagttacc
    36781 tccgccgtgc acatggcttt gcagtttcgc ttgatagtga tgacgtcgaa acccgtatga
    36841 tgagcaacta tacgctgaag cgtgaactcg ccgagtttgc cgcgtcactg gttcaaccgg
    36901 gcgaaaccat ctttatcgaa aatggcagca gcaatgccct gctcgcccgg actctggggg
    36961 agcagaagaa aaatgtcact atcatcacgg tcagcagcta catcgcgcat ttgctgaaag
    37021 acgcgccttg tgaagtcatt ttgctcggtg gcgtgtacca gaaaaaaagc gaaagtatgg
    37081 ttggcccgtt gacacgccag tgcatccaac aggtgcattt cagcaaagca tttattggta
    37141 ttgatggctg gcaacctgaa actggattca ccggtcgcga tatgatgcgt accgatgtgg
    37201 tcaatgccgt gctggaaaaa gagtgcgaag cgatagtcct gactgacagc tcgaaatttg
    37261 gtgctgtaca ttcatactcc atcggtcccg ttgagcgatt caatcgcgtg attaccgatt
    37321 cgaaaatacg cgccagcgat ctgatgcatc ttgagcacag taaactcacc gttcacgtcg
    37381 ttgacattta accatttccc ttctctacgg atgatttgca gtttggcaaa tcatccgctc
    37441 taagatgatt cctggttgat aattaagact atttacctgt tattaacacg cttaagatat
    37501 aaaattatta tcagcgatat aacaggaagt cattatcacc tgcgtgatat aaccctgcgc
    37561 gcgagcagat ttcacggaat aatttcacca ggcttattct tagctattat agttatagag
    37621 agcttacttc cgtgaatcat aaattcagga gagagtatta tgtttgtaac gagcaaaaaa
    37681 atgaccgcgg ctgttctggc aattactttg gcaatgtctc tgagtgcctg ttctaactgg
    37741 tctaaacggg accgcaacac cgcaatcggc gcgggtgcag gggcattagg cggtgcagta
    37801 ctgaccgatg gcagtacgtt gggtacatta ggtggtgcag ctgtcggtgg tgttattggt
    37861 caccaggtcg gtaaataatc gtaaataaat gtcgattatc gactcgcaat tataatacgc
    37921 ttgattctgt tcagtacgaa ataactgaat aatacgcaca gtaaaatatt cgaaagccac
    37981 ggatatatcc gtggcttttt atgttaaccg cctgcgagtt ttaccttcat ccctttcgct
    38041 tcaagtagtg attttaataa atcacgctta tcgccctgga tttcaataac tccatcttta
    38101 actgctccgc cgcagccgca ttttttcttc agttccgctg caagttttgt cagttcggca
    38161 tcatcgagat cgacaccggt aatcaggcaa acgcccttac ctttacgtcc actggtctga
    38221 cgctgaatac gcaccacacc gtcgccttta gggcgtacag gggccgcttt gggctcatca
    38281 atacgtccgg tttccgttga gtagaccaga cggctgttgg aatcactcat catgcactcc
    38341 gctgtaaaga ggcgttgatc gctttcagcg tctgcgctgg atctaccgat tgcgttaccg
    38401 ggcgaccaat caccatataa tcaacaccag ccgacaacgc ctgttctggc gtcataatgc
    38461 ggcgctggtc accagcttca ctcccctgcg gacgaatgcc cggcgtaacc agtttgaact
    38521 cctgaccgaa tacctgttta aagcgcacag cttcctgagc agaacacacc acaccatcaa
    38581 ggccacattt ttgcgtcagt gccgccagac gttctgcata atctgcaggt gacagtgtca
    38641 tgccaagatc gaccaggtcg ctggcttcca tgctggtcaa cactgtcaca gcaatcaaaa
    38701 gcggtgcatc tttgccaaac ggaaccagtg cctcacgcgc tgcggtcatc atacgcgccc
    38761 caccagaggc atgaacattc accatccaca cgcctaagtc agctgcagca gcgacagcgt
    38821 gcgctgcagt gttaggaata tcgtggaatt tcaggtcaag aaagatatca aaaccacgct
    38881 gttgaagttc gcgcacaaac tgtggcccaa acaatgtaaa catctctttg ccgaccttca
    38941 gacgacaatc gcgtgggtcg atcttgtcga caaaggacag cgcgtcatca cgattatgat
    39001 aatcaagggc aacaaccaca ggagaattcg taacagcgcg ggaagaagat gaagcagtta
    39061 acgtcatgac cagaccttct tgatgatggg caccggaagt ggcgcgaaac aggtaaacgg
    39121 cgagcattct acccgtggaa ataaaaaatt aacagttgcg atttttcccc tggcaggctt
    39181 tttctgctcc gtcgttgccg gagcacaatg gaacatcaat aactagtatg ttgtaactaa
    39241 agtcggattt ttttaaaaat tacaggccat caagaccgcg aatcggttta atggttgacc
    39301 aggcccgaca agacggacaa tgccagtaga gggtgtatgc ggtaaaacca catttctggc
    39361 agcgataacg aggcttacta cgtacctttt cgccaaccat gtcacgcagc accatcaggc
    39421 tctctttggc acgcccttct tccgcttcat ttaagtggta atccattaac ttatggaaca
    39481 cacgcatggt cggatgacgc tgaagctggc gcgtaatata gacctgtgcg gcctcactac
    39541 cgtcgcgcgc ttcgatgata tcagcaagca tcaattcagc atcggcaccg gtgttctctt
    39601 ccaccgcgcg ttgcaggaat tctgcccatt cggcagtttt acccaactgc tgatagcagg
    39661 tttgcaacat ttccagcgtt tcgctgacca gttctctgtc ctgggatatc acgcgttgca
    39721 aactttcgac ggctttggcg tattctcctt tcgccataaa cacgcgtccc atcatgatgg
    39781 atacgcgggc gctgttttta tctgccgccg cccctttttt tagcaaggtc atggcacgat
    39841 cgagatcgtc gctggccata tgctgcaggg ctaactcaca gtagaaatgg gcaatttcga
    39901 cgcgctgttt atctttaccc agcttcacca ggcgttcggc aacatcaatt gctttctgcc
    39961 actcgctggt agcctggtag atttgtagca actgttgcag cgcgccaatg cggaagtcag
    40021 tttcatcggt cagctgattg aacatgtctt ccgcgcggtc gtataacccg gcggccatgt
    40081 aatcacgccc cagttgttga atcgccaaca gacgctgttc ataggtcagt gaggcgcttt
    40141 ccattagggt ctgatggatg cgaatagcgc gatcaacttc gccacgcgaa cggaacaggt
    40201 ttccgagcgt aaggtgggct tcaacggtgc ctgtatcctc tttaagcata tcgagaaaca
    40261 ggtctaccgc tttatcctgt tgattactaa gcaggaagtt aacccccgct acgtaatcac
    40321 gcgacaagcg gttggcttca tcttgcttgt tttgttgcgc acttctgcgg cccatatacc
    40381 agccataggc agcggctaca ggcaaaagca gaaacaacaa ctccagcata gaaagttatt
    40441 ccttcgccgc tgacgagtgc ggcactacag ccacgtcagt cgcgggtgaa agctggtttt
    40501 ccagtcgctt tattttacgt tcagcgcgcg ccagggaaac acgaactcgc agccagaaca
    40561 ggccacaaat caaccaaccg atagcaaccc ccgcagcaaa caataccgcc agcaatgtgg
    40621 aaatacggta ctccccttgc gctaacagat aattaaacgt cacctgttga tcgttctgcg
    40681 cacccaacgt gaccgaaatc acgaagatcg ctaacaccag taagaaaatg agtaaatatt
    40741 tcacattact tcccgttatg tggttaaagc gaataaatta tggtcaactt accgcaatag
    40801 ccctttaaag taccattttc gcgccgtctg cgaaatggca tccacgcgca ttgttgatga
    40861 tttaagggct aaattcggaa aatcaaccag cgttaacttt cttgttctcg ttgcgctatt
    40921 tcgcgatttt cttccgcagg tggtgttaat ggcccacaaa ttcgttgcgc aagccacgtt
    40981 gccaccgcca ccagcgccca cgaaatcaac gtagctacta ccagatcgcg tggccaatgc
    41041 atcccgagca gcaggcggct tcccatgact cccgttgccc agaccagcaa gatagcaatg
    41101 gttaacgttc gccgacgcgg ccacagcaaa ccaacggcca gcagtgccca actggcagca
    41161 aacatcgtgt gaccggaagg aaaggcaaac cccgtctctt tctgccagtg tgaacgcaaa
    41221 tattgtggga tatttttctc ttcagccaac tgttctttca ctagatttcc gcgttctgct
    41281 cgctttaaag tgtagaactc atcaaccgga atatgatgtg ttttttccag ccagataaca
    41341 aaaggtcgtg gttcctggac tttgtctttg atccaggatt taacgccttg tcccacaatg
    41401 attacggccg ccagaatggc aaataataca aaggcagcct taatgcaaaa acgcagacac
    41461 cagagaaacc agccgaataa aatcaaatgt gtaatgacgc cccagggctg ggtgacagtt
    41521 tcagtaaccc aaaaagccgc ttttagtagc caactttgtt ctccaggttg ccaacgccag
    41581 ccagaaatcc atacggctac tggcatgaca agcaatagtg cagctcccac tgcggtacgt
    41641 ctggcaatcg aacgcatggc ctctcctttt tgataagtcc cacaatcata actgaaaacg
    41701 ccagttccag gaaaaattga cagatttgtg ccattccgtg aacgatcgac gcgtcgtgat
    41761 taggtgaacc ccttctcgtt atggcaaaat aagccaatac agaaccagca ttatctggag
    41821 aatttcatgc agcttaaacg tgtggcagaa gccaaactgc caaccccatg gggcgatttc
    41881 ctgatggtgg gatttgaaga actggcaacc ggacacgatc atgtcgcgct agtctatggc
    41941 gatatttccg ggcatacccc ggtacttgcg cgcgtccatt ccgaatgtct gaccggtgac
    42001 gccctgttca gcttgcgctg cgattgtggc ttccagctcg aagcggcatt gacgcaaatt
    42061 gccgaggaag gccgtggtat tttgctgtat caccgtcagg aaggtcgtaa cattggtctg
    42121 ctgaataaaa tccgcgctta cgcactgcag gatcaaggtt acgataccgt agaggctaac
    42181 catcagttag gcttcgccgc tgatgagcgc gacttcactc tttgcgctga tatgttcaaa
    42241 ctccttggcg tcaatgaagt ccgcttgtta accaataacc cgaaaaaagt cgaaattctg
    42301 accgaagcag ggattaatat tgttgaacgc gtaccattga ttgtaggtcg taaccccaat
    42361 aacgaacatt atctcgatac caaagccgag aaaatgggtc atttgctgaa caaataaccc
    42421 tctcgcattg tggaattaat ttgcttgccg gaagcaaaat aaccggcaag caaatatttg
    42481 ttacttcagc atattacgaa tgacataatg caaaatgccg tcgttctggt agtaggtcaa
    42541 ctccgtcgcg gtgtcgatac gacaacggca gggtacgacc tcctggctac catccgcgcg
    42601 cgtaagcgtc accggaaccg tcgcaccggg ttgtagattt tgcagatcgc caatatcaat
    42661 cttctcttcc ccggttagtc ctaacgtttt acgcgttacg ccttgcggaa attccagcgg
    42721 caggatgccc atgccaatta aattcgaacg gtgaattcgt tcaaacgatt cggcaatcac
    42781 cacacgaatt ccaagcagac gaggaccttt tgccgcccag tcacggcttg agcctgaacc
    42841 atactctttc ccggcaatca ccgccagcgg cgtttgttcc tgcttatagc gcatcgcagc
    42901 atcataaata gagactacgt cgctgtcagg taaatgccgc gtcatccccc cttcaacgcc
    42961 aggcaccatt tcattacgga tgcgaatatt ggcgaaggtg ccgcgcatca tcacttcatg
    43021 gttaccacgc cgcgaaccgt aggagttaaa gtcttttcgc tcaacacccc gaccttgtag
    43081 atatcgaccc gctgggctgt cgggcttaat actgcccgcc ggagagatat ggtcagtggt
    43141 gactgaatcc cccagcattg cgaggatccg cgcaccgtga atatcttcca ctggtgctgg
    43201 tgttgcctgc atttcatcaa agaaaggcga taagcgaata taggttgagt cctcctgcca
    43261 accgtaggta tcggatcgtg tgacgttaat ttccttccac tctgctgtgc cttcaaaaac
    43321 ttctgcgtac tctttgcgga acatttctgt ggagacttgt tctaccgcac gggcaatttc
    43381 ttgtgccgat ggccagatat ctttcagata aaccggatcg cctttacgat catgaccaat
    43441 aggctcagaa gccaggttga tattcatatt tcccgccagc gcataggcaa ccaccagcgg
    43501 cggcgaggcc agccagttgg ttttaaccag cggatggata cggccttcaa agttacggtt
    43561 gccggacagc actgcaccga cggttaaatc gccttttttg attgccgttt cgataggatc
    43621 gggcagcggc ccagagttac caatacaggt agtacaaccg tatcccacaa ggttaaaccc
    43681 cagttcgtcg agaaacggtg tcagtttcgc ttttgccaga tagtcagaaa caactttcga
    43741 gcctggtgcc agcgacgctt tgacccacgg ttgccgcttg aggccaagaa ttacggcttt
    43801 tttcgccagc aagcctgcag ccatcagcac acttgggtta gaggtgttgg tgcacgaggt
    43861 tatcgcagca atgaccacag cgccatcagg taactgatac tgatgtccgt tcataacata
    43921 atcgaccggc tggcgatctt tatgcgtggc attcacttcc agttcgttac tggcggcaaa
    43981 tacttttggt acatcgggca gtgcaacgcg atcctgtggg cgttttggcc ctgccaggct
    44041 cgcttcaacg tcattcatat ccagttctaa cgtactggta aaaatcggtt catcgcccgg
    44101 gttacgccac atgccctgcg ctttggcata tttttcgacc aactcaacct gatcttcgct
    44161 gcgcccgctt aaacgcatgt aatcgagggt tacagcatcg attgggaaga agccacaggt
    44221 ggcaccatat tctggcgaca tattggcaat ggtggcgcga tccgccagcg gtagtgaatc
    44281 cagaccatca ccataaaatt cgacgaattt ctccaccacg ccatgtttgc gcagcatttg
    44341 ggtaacagtg agaaccaggt ctgtggcggt aataccttca cgtaatttac cggtaagttt
    44401 aaagcccact acgtccggga taagcatgga aaccggctgg cctaacat
//
LOCUS       DADMKF010000012        43713 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.012, whole genome shotgun sequence.
ACCESSION   DADMKF010000012 DADMKF010000000
VERSION     DADMKF010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 43713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43713
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.012"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(<1..559)
                     /locus_tag="JNC24_000640"
     CDS             complement(<1..559)
                     /locus_tag="JNC24_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HAY9157474.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPDTARDFLAFHLPAPIHALCDMKT
                     LKLESSSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVLLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQR"
     gene            complement(762..998)
                     /gene="feoC"
                     /locus_tag="JNC24_000641"
     CDS             complement(762..998)
                     /gene="feoC"
                     /locus_tag="JNC24_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HAY9157475.1"
                     /translation="MASLIQVRDLLALRGRMEATQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            complement(998..3319)
                     /gene="feoB"
                     /locus_tag="JNC24_000642"
     CDS             complement(998..3319)
                     /gene="feoB"
                     /locus_tag="JNC24_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HAY9157476.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTSARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDAALARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGDGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            complement(3336..3563)
                     /gene="feoA"
                     /locus_tag="JNC24_000643"
     CDS             complement(3336..3563)
                     /gene="feoA"
                     /locus_tag="JNC24_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HAY9157477.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFNVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            complement(4001..6322)
                     /locus_tag="JNC24_000644"
     CDS             complement(4001..6322)
                     /locus_tag="JNC24_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HAY9157478.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYIDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     GVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKVAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFKNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNARRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            complement(6419..6931)
                     /gene="greB"
                     /locus_tag="JNC24_000645"
     CDS             complement(6419..6931)
                     /gene="greB"
                     /locus_tag="JNC24_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709179.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HAY9157479.1"
                     /translation="MRIIKQTKGINEMKTPLVTREGYEKLKQELNYLWREERPEVTKK
                     VTWAASLGDRSENADYQYNKKRLREIDRRVRYLTKCMENLKIVNYSPQQEGKVFFGAW
                     VEIENDDGVTHRFRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEASW
                     YVNAIEYVKP"
     gene            7123..7842
                     /gene="ompR"
                     /locus_tag="JNC24_000646"
     CDS             7123..7842
                     /gene="ompR"
                     /locus_tag="JNC24_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018150685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HAY9157480.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            7839..9191
                     /gene="envZ"
                     /locus_tag="JNC24_000647"
     CDS             7839..9191
                     /gene="envZ"
                     /locus_tag="JNC24_000647"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HAY9157481.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(9267..10889)
                     /gene="pckA"
                     /locus_tag="JNC24_000648"
     CDS             complement(9267..10889)
                     /gene="pckA"
                     /locus_tag="JNC24_000648"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HAY9157482.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            11268..12968
                     /locus_tag="JNC24_000649"
                     /pseudo
     CDS             11268..12968
                     /locus_tag="JNC24_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709174.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
     gene            complement(13103..13981)
                     /gene="hslO"
                     /locus_tag="JNC24_000650"
     CDS             complement(13103..13981)
                     /gene="hslO"
                     /locus_tag="JNC24_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HAY9157483.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNAQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(14006..14407)
                     /gene="hslR"
                     /locus_tag="JNC24_000651"
     CDS             complement(14006..14407)
                     /gene="hslR"
                     /locus_tag="JNC24_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HAY9157484.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMALAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            complement(14418..15086)
                     /gene="yrfG"
                     /locus_tag="JNC24_000652"
     CDS             complement(14418..15086)
                     /gene="yrfG"
                     /locus_tag="JNC24_000652"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HAY9157485.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            complement(15151..17286)
                     /gene="igaA"
                     /locus_tag="JNC24_000653"
     CDS             complement(15151..17286)
                     /gene="igaA"
                     /locus_tag="JNC24_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HAY9157486.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIYTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDGSRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMKIQAYYESCLNPQLITPSESLIE"
     gene            17606..18166
                     /gene="nudE"
                     /locus_tag="JNC24_000654"
     CDS             17606..18166
                     /gene="nudE"
                     /locus_tag="JNC24_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HAY9157487.1"
                     /translation="MSKSLQKPTILNVETVAHSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            complement(18331..20883)
                     /gene="mrcA"
                     /locus_tag="JNC24_000655"
     CDS             complement(18331..20883)
                     /gene="mrcA"
                     /locus_tag="JNC24_000655"
                     /EC_number="2.4.1.129"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HAY9157488.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVATLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQEATAMLADGSTVALSMDGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGVGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            21003..21782
                     /gene="hofM"
                     /locus_tag="JNC24_000656"
     CDS             21003..21782
                     /gene="hofM"
                     /locus_tag="JNC24_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HAY9157489.1"
                     /translation="MAFKIWQIGLHLQQQEAVAVAIVRGAKECFLQRWWRLPLENDII
                     KDGRIVDAQRLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRCRWGRKLAVGMTSAKELAAALSVDPES
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKAY"
     gene            21782..22114
                     /locus_tag="JNC24_000657"
     CDS             21782..22114
                     /locus_tag="JNC24_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial assembly protein"
                     /protein_id="HAY9157490.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTD
                     NAEARVNTVLLQAEQQLARSLQITKPRLLERQQIREQRLQRQRQRQFTRDWQSALEAL
                     AALLPEHA"
     gene            22305..22745
                     /gene="hofO"
                     /locus_tag="JNC24_000658"
     CDS             22305..22745
                     /gene="hofO"
                     /locus_tag="JNC24_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HAY9157491.1"
                     /translation="MNMFFDWWFATSPRLRQFCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWATLYRLVDTTPFSEEKTLPFSPLDFQLPGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLETPHEG"
     gene            22735..23139
                     /gene="hofP"
                     /locus_tag="JNC24_000659"
     CDS             22735..23139
                     /gene="hofP"
                     /locus_tag="JNC24_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HAY9157492.1"
                     /translation="MRVKRWLLAGIVLCLLTGMRDPFKPPEDLCRISELSQWRYQGMV
                     GRGERIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDVLTLGTGTNCEPPQWLWQRQ
                     GDTNEAMDSRTTVDADTRRTGGKAAKSDADGG"
     gene            23051..24289
                     /gene="hofQ"
                     /locus_tag="JNC24_000660"
     CDS             23051..24289
                     /gene="hofQ"
                     /locus_tag="JNC24_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HAY9157493.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNNNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            24690..25211
                     /gene="aroK"
                     /locus_tag="JNC24_000661"
     CDS             24690..25211
                     /gene="aroK"
                     /locus_tag="JNC24_000661"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HAY9157494.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            25268..26356
                     /gene="aroB"
                     /locus_tag="JNC24_000662"
     CDS             25268..26356
                     /gene="aroB"
                     /locus_tag="JNC24_000662"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HAY9157495.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTNET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLKRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEIRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            26448..27734
                     /gene="damX"
                     /locus_tag="JNC24_000663"
     CDS             26448..27734
                     /gene="damX"
                     /locus_tag="JNC24_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HAY9157496.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTSSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPAATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPSTTRPARQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPTATSTPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETARNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            27841..28677
                     /gene="dam"
                     /locus_tag="JNC24_000664"
     CDS             27841..28677
                     /gene="dam"
                     /locus_tag="JNC24_000664"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HAY9157497.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKTYFPEAELYHFAEKAQNAFF
                     YCESYADSMARADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            28695..29372
                     /gene="rpe"
                     /locus_tag="JNC24_000665"
     CDS             28695..29372
                     /gene="rpe"
                     /locus_tag="JNC24_000665"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAY9157498.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            29365..30123
                     /gene="gph"
                     /locus_tag="JNC24_000666"
     CDS             29365..30123
                     /gene="gph"
                     /locus_tag="JNC24_000666"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HAY9157499.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPIAGEE
                     RVITWIGNGADVLMERALTWARQERATQRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            30116..31120
                     /gene="trpS"
                     /locus_tag="JNC24_000667"
     CDS             30116..31120
                     /gene="trpS"
                     /locus_tag="JNC24_000667"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HAY9157500.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
     gene            31280..32185
                     /locus_tag="JNC24_000668"
     CDS             31280..32185
                     /locus_tag="JNC24_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9157501.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLTQKGLCLALE
                     LGPHTYVGEHQLICRKGESANVKRVGLDNRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            32202..32564
                     /locus_tag="JNC24_000669"
     CDS             32202..32564
                     /locus_tag="JNC24_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAY9157502.1"
                     /translation="METRLNLLCEAGVIDKDICKGMMQVVNVLETECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQVLLKEFALEVNPCEE
                     GYLLANLYGLWMAANEEV"
     gene            32648..33811
                     /locus_tag="JNC24_000670"
                     /pseudo
     CDS             32648..33811
                     /locus_tag="JNC24_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417840.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
     gene            33811..35037
                     /locus_tag="JNC24_000671"
                     /pseudo
     CDS             33811..35037
                     /locus_tag="JNC24_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417839.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
     gene            35034..35912
                     /locus_tag="JNC24_000672"
     CDS             35034..35912
                     /locus_tag="JNC24_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HAY9157503.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLGVMRETGINVVACTGYYQDAFFPEHVVTRSVQELA
                     QEMVDEIEQGIDGTELKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRETPSQFFQ"
     gene            35923..36276
                     /locus_tag="JNC24_000673"
     CDS             35923..36276
                     /locus_tag="JNC24_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HAY9157504.1"
                     /translation="MKKIGVAGLQREQIKKTIEATATGSFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            36288..37592
                     /locus_tag="JNC24_000674"
     CDS             36288..37592
                     /locus_tag="JNC24_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9157505.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIRPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLV
                     AAVVVLMTRVVVMRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPIVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            37604..38689
                     /locus_tag="JNC24_000675"
     CDS             37604..38689
                     /locus_tag="JNC24_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HAY9157506.1"
                     /translation="MKTFPLQSLTIIEAQQKQFALVDSICRHFPGSEFLTGGDLGLTP
                     GLNQPRVTQRVEQVLADAFHAQAAALVQGAGTGAIRAALAALLKPGQRLLVHDAPVYP
                     TTQVIIEQMGLTLITADFNDLSALKQVVDEQLPDAALVQHTRQQPQDGYILADVLATL
                     RSAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVISRIRS
                     TLYSGGSQIQGAQALEVLCGLVFAPVMHAVQAGVSERLLALLNGGAVPEVKSAVIANA
                     QSKVLIVEFHQPIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQSE
                     HCAIRINPNRNGEETVLRILRESIASI"
     gene            complement(38841..39572)
                     /gene="phoB"
                     /locus_tag="JNC24_000676"
     CDS             complement(38841..39572)
                     /gene="phoB"
                     /locus_tag="JNC24_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator PhoB"
                     /protein_id="HAY9157507.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKATK
                     EKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(39672..40457)
                     /gene="frlD"
                     /locus_tag="JNC24_000677"
     CDS             complement(39672..40457)
                     /gene="frlD"
                     /locus_tag="JNC24_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HAY9157508.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGCI
                     TLVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSPLWQTLVPHL
                     DFAFASAPQEDEALRLKMKAIVARGAGTVIVTLGENGSIAWDGAQFWRQAPEPVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHGAW"
     gene            complement(40454..41285)
                     /gene="frlC"
                     /locus_tag="JNC24_000678"
                     /pseudo
     CDS             complement(40454..41285)
                     /gene="frlC"
                     /locus_tag="JNC24_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026213.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
     gene            complement(41335..42357)
                     /gene="frlB"
                     /locus_tag="JNC24_000679"
     CDS             complement(41335..42357)
                     /gene="frlB"
                     /locus_tag="JNC24_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HAY9157509.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHAIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPNAEIGKIKNDLKPLPNALGHLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            complement(42378..>43713)
                     /gene="frlA"
                     /locus_tag="JNC24_000680"
     CDS             complement(42378..>43713)
                     /gene="frlA"
                     /locus_tag="JNC24_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
                     /protein_id="HAY9157510.1"
                     /translation="GSQELQRKLGFWAVLAIAVGTTVGSGIFVSVGEVAKAAGTPWLT
                     VLAFVIGGLIVIPQMCVYAELSTAYPENGADYVYLKNAGSRPLAFLSGWASFWANDAP
                     SLSIMALAIVSNLGFLTPIDPLLGKFIAAGLIIAFMLLHLRSVEGGAAFQTLITIAKI
                     IPFTIVIGLGIFWFKAENFAAPTTTAIGATGSFMALLAGISATSWSYTGMASICYMTG
                     EIKNPGKTMPRALIGSCLLVLVLYTLLALVISGLMPFDKLANSETPISDALTWIPALG
                     STAGIFVAITAMIVILGSLSSCVMYQPRLEYAMAKDNLFFKCFGHVHPKYNTPDVSII
                     LQGALGIFFIFVSDLTSLLGYFTLVMCFKNTLTFGSIIWCRKRDDYKPLWRTPAFGLM
                     TTLAIASSLILVASTFVWAPIPGLICAVIVIATGLPAYAFWAKRSRQLNALS"
BASE COUNT        10157 a        11128 c        11988 g        10440 t
ORIGIN      
        1 cgcgttgacg aatatgtttt tgaattaact ccagcagcgc catacgtcgg tgctgcatga
       61 tttcatcatc aggcatgacg gtgacatcaa tcagcggaaa tgcggaggca taaagctgcc
      121 ttgccagttt gggatcggcg aaacaatcca gccaacacag cgaataggga taggggcttt
      181 caataccgtg gtaaaacaac aatagcacca ccatcggcaa cgttttgtat ccagcatcaa
      241 ggtgattttg cattgcggca atagcgtaac gcatcatgcg aaatgcgatc agtttgtttg
      301 aggtgctttg atgttcaatc agacaataga tgtatcctgg tccttgttcc gttttcaccg
      361 accacagcac atcggaatag ctttcacgca gatcgtcatc aataaagctg ctcgactcca
      421 gcttgagggt tttcatatca caaagcgcgt gaatgggtgc cggtaaatga aacgcaagaa
      481 aatcacgagc cgtgtccggt tggcgtaaaa agagtttgaa cagcgcatcg tgtggggtgg
      541 aactctgctt tttgctcatg gcgttccttc acctgttgtt tgaatgccgc gactctacgc
      601 gccactccac acctgtgcat tagcgagttt tcatccctgc gagatgcttt caaaggaact
      661 tacgccgtgc tgcaagagtt gcatttttat ggaaagccgc acgcaaaaaa gcccggatgc
      721 agacgcatac cgggctttcc aaaacaggcg atggagtaag gttaacgcag cgcccaccac
      781 tcgcgcagac aggcttttcc ttccgggcag cttttacaac tgccagagag gcagccgtca
      841 ggttcttcct gaatccgcac ggctttgccc atactttcca gttgttgcag catggcgtta
      901 atcattggct gcggagtgtt caatgtctgg cttatctggg tcgcttccat acggccccgt
      961 aacgccagca aatcacgtac ctgaataagt gaagccatta atggcaatca ccggtggtgc
     1021 tggctgcgca gcaactgctt accgacttgc gggttgccag cagttcgata tccacccggc
     1081 tacgcgcgcg gcgcagcaga ccgataacta cgatgttaaa caggattacc gccagaatgc
     1141 acaccaggct gtaagttggg tgctgactgt agctggcgac ctgatagaac aatgttgcca
     1201 gtgagtaagc gatattcagc ccccacagga tggagaagcc catccagcca cggcttgatt
     1261 cacgggcgat ggctcccatc accgagatac atggtacata cagcaggacg aaaatcaggt
     1321 agctgtatgc ggcagctgcg ctaccgaatt tctgatccat cacgcccatc gccccggtac
     1381 ccatttcacc gtcgcctttg ctagcttcaa tggggttcat cagtacgcta aggctgaagg
     1441 tgtctttcag gctctgccag gtttcatcta ccgcactgaa cagctcttca ccgaggttga
     1501 attccgccgg attgaactct tcgtcctgaa tattttctgc ggtgtagagg gtgttgagcg
     1561 tacccaccac cacttctttt gccattgcgc ccgtaaacag gccaaccgtt gcctgccagt
     1621 tatcttcatg tacgccaatc ggcttgaaga caggggttat cacccggctg acggacgcca
     1681 gcgccgagtc gttgatgtta tcgacgattt tcccgctcaa cgagaagctg ttgaaagcgc
     1741 tcaggaaaat gctgacgatg ataatcactt taccagcacg cagaacgaag cccttcagac
     1801 gctgccaggt ctggataatc aggcttttaa cgtgtggtac atggtagacc ggcagctcca
     1861 tgacaaacgg cgtcgcttca ccgcgcatga tggtgtactt gagcatcagg ccagtcagca
     1921 ccgccatcac gataccgagc atatacagcg agaaaaccgc cagggcaccg ttctgcccga
     1981 agaaggcagc cgcgaatact gcgaagatag ccagacgcgc gccgcaggac ataaacggtg
     2041 ccatcatgat ggtcatcaga cgttcacgcg gtgcatcaag cgtacgtgcg cccattaccg
     2101 acggtacgtt acaaccgaaa ccgacgatca gcggcacaaa ggatttcccc ggcaagccca
     2161 gcgcctgcat caggcggtcc atcacaaacg ctgcacgcgc catatagccg gagtcctcaa
     2221 ggaaggagag gaacaggtac atcatgccga tctgcggcac cagtggcagc acggtgttaa
     2281 taccgccacc caggccctgg gcgaggaaga tagtcagcca gtccgggaag tggagcgtgt
     2341 agccaatcca ttgaatacca tgcacaaata gcgccacgga gccgacgtca aacagcggct
     2401 gtaacgcccc gccgatgttg atagccagca ggaacatcag gtacatcaca aagaggaaaa
     2461 tcggcagacc gaggaaacgg ttgagcacga ttttatctac cgcagtggtg aaacggctgg
     2521 gttctgccgt cagggtgttg cttaccacat cacagatggc agctatgcac tggtaacgcg
     2581 catcggcaat gtgcagcgcc ggatcatcca tttcattacg cagacgggca agggcggcat
     2641 ccagatgctg cgacgcttca ccggcgtagg cgcggctgta gatatcgcct tccagcattt
     2701 gcaggcccag ccagcgacgt tgtttcagcg ggatgtcgga aggcatcact tttgccagtg
     2761 aatctgcttc gttgagtagc ggctgtgcgt aatgcaccag ttccacattc tcgttagctt
     2821 tatagcgatc aatcgccagc ttgagcgctt caataccgcg accacgggtt gaaaccagcg
     2881 ggatcactgg gcagccaaga cgcgccgaca gagcatcaat ttcaatacga atattttgct
     2941 tctcggcaat gtcgagcatg ttcagtgcca cgatgcaggg aatgccgagt tccagcagtt
     3001 gtagcgtcag gtacaggtta cgctcaaggt tagacgcatc caccacgtta atcagcaggt
     3061 cggcgtcgcc actcaaaatg tagtgacagg cgatttgctc atcgagcgag gtctgcgatg
     3121 agatggtggt cagagaatag gtgccgggca ggtccaccag cgtgacctga tgatcggtgg
     3181 tggagaattg cccttcttta cgttcgacgg taacgccagc ccagttacct acacgctgac
     3241 gtgcgctagt gagctggtta aataaggttg tcttgccaga atttggatta ccaattaagc
     3301 caatggttaa ttttttcatt gttgttatca ccgtattaac aggaaaccgc ttccacttct
     3361 aataaggcca gatctttttt gcgtaatacc aggctcacac gacgggtttc gatatgaatg
     3421 gggtcgccga gtggagcgac gcgcaccaca ttaaaagagg agccaggtaa catgccaaga
     3481 gaaagcagtt tttggcgata tgccgggctg atttcacggg aaaagccagt gattttccac
     3541 gcagtatctg gagtgtattg cataggtgcc tacttgtttc tcattaactg gataactacc
     3601 tcatggggcg tttgacagcg acatgagacc agatgatttg ccaacaaagg agagattaaa
     3661 taatcaacaa cacaatgata atgagaatgg tttctatcag caatacttaa aatgtgtgcg
     3721 aatgttggtt tgaataataa tctgactcaa tgttgatgtg gctcaaggtt atatcgcagc
     3781 gaaaatataa ttcgaatagt gaattacccg gactggctaa tgtttaatta aggtttcagt
     3841 tgttaatgaa gtgataaata ttgccggaat atattatgag tttggttgtg gaataaaaag
     3901 gccggagata ttctccggca cttttatgtc aggcaaggcg ttcgttgcca gatgcggcgt
     3961 gaacgcctta tcgcgcaggt ttaggggcca gtccgggcgt ttaacgtttt ttgcccattg
     4021 ccgccgccag cgcatccatc atcgcgctat taccggcagg ctgcgcttca cgaccgcgtg
     4081 gtttggctgc cgggcggttg ttttgcgggc gttcattacc gccgccgcga cgggcgttgg
     4141 tttcgccagg ctgctcgtcc agacgcatgg tcagggcgat acgtttacgc tgaagatcca
     4201 cttccagcac tttcaccttc acaatgtcgc ccgctttcac cacggtatgc ggatcttcca
     4261 caaacttgtt cgacaatgaa gagatgtgaa ccaggccgtc ctgatgcacg ccaatatcga
     4321 caaacgcgcc aaagttggtg acgttggtga cagcgccttc gaggatcata cccggttgca
     4381 ggtcgttcat tgtctcgacg ccatcggcaa actgagcggt tttaaattcc ggacgcggat
     4441 cgcgacccgg tttttccagc tctttgatga tgtcagttac tgtcggcaca ccgaattttt
     4501 catcagtaaa gtcagacgct ttcaggttac gcagttcgct gctgttaccc atcagatctt
     4561 tcagtgcctg ctgtgttgct gccagaatgc gttccaccac cggataggct tccgggtgaa
     4621 cggtagaggc gtccagcggg ttatcaccgt ggttaatgcg caggaagccc gcgcactgct
     4681 cgaaggcttt cggccccaga cggctgactt tcagcagttg ctgacggttc ttgaactggc
     4741 cgttctcatc gcgccaggcc acgatgtttt gcgccatcat gcgcgtcagg cccgccacgc
     4801 gagttaatag cggaacagaa gcggtgttga gatcgacgcc aacggcgttt acgcagtctt
     4861 ctactactgc atccagtttg cgggccagtt gcgtctggct gacgtcatgc tgatactgac
     4921 ctacgccgat agatttcgga tcgattttca ccagctccgc cagcggatcc tgcaaacggc
     4981 gggcgataga taccgcacca cgcagcgaaa cgtcgagatc cgggaactcc tgcgctgcca
     5041 gctcggaagc tgagtaaacc gacgcgccag cttcgctgac gatcactttc tgtgcggtca
     5101 ctttcgggaa ctgcttctgc acgtcgagat agaaacgttc agtttcgcgg gaagctgtac
     5161 cgttgccgat cgccaccagt tcaacgttat gtttttcaca caaggcagca acggtcatcg
     5221 ctacttttgc ggcttgtccg gtgtgcgggt aaatggtatc ggtcgccacc agtttgccag
     5281 tggcatcgac cactgccact tttaccccgg tacgcagacc cggatcgagg cccatcgttg
     5341 cacgcagtcc ggcaggggcc gccatcagca gatcgtgcag gttacgggca aagacgttga
     5401 ttgcttcatc ttccgcgcgt tcgcgcacgg tacccatcag ttcggtttcc agatgcatca
     5461 gcaccttgat gcgccaggtc cagctcacta cgcctttgcg ccagctatcc gccggggcat
     5521 tgttcaggcg caggccaagg tgatccatga tgatttgctc gcaatagctc tctttgggcg
     5581 gctcatcgaa ctgcggatcg gcattcagcg aaagctggag tacgccttcg ttacgcccac
     5641 ggaacatcgc cagcgcgcgg tgagaaggca ccgtggacaa cggttcgtga tgatcgaaat
     5701 agtcgcggaa tttcgcccct tcctcttctt taccgctcac caccgtagaa accaaatgcg
     5761 cgttcttcca cagataatca cgcactttcg ccagcagcgc ggcatcttcg gcaaaccgtt
     5821 ccatcaggat atagcgcgcg ccgtccagcg cggctttggt atctgccacg cctttatcgg
     5881 catcaatata ttgcgcagcg gcgacttctg gtgtgtgtga cggatcgctc cacagcaggt
     5941 cagccaacgg ctcaagccct gcttcaatgg cgatttgccc gcgggtgcgg cgtttaggtt
     6001 tgtagggcag gtagaggtct tcgagttcgg ttttgcttag ggtggcgttg atggccttcg
     6061 ccagatcatc ggtgagtttg ccttgctcgg aaatggactt gaggatcgcc tgacgtctct
     6121 cttccagctc gcgcagatag ctcagacgcg tttccagatt acgcagctgc gtgtcatcca
     6181 gaccgccggt gatttcctta cgataacgtg cgataaacgg cacggtattc ccttcgtcaa
     6241 gcaggcgaac ggcagcgtca acctgttccg ggcgcgcctg aatttcaccc gcaataatgc
     6301 ggcagaacga atcattcatc atggtttgag ttcatctttt cggatcaaaa atcaggggat
     6361 agttatacgg actggctggc aaaaatgcca gccatcggca ggaggttaag actcttcatt
     6421 acggtttcac gtattcgata gcattaacat accagctcgc ttccccggca ggggtattca
     6481 ccaccgccag atcgccgact tcttttttca gcaatgcgcg ggccatcggg gaatcgatag
     6541 agatgtaatc tttacggcca aaaatttcat cgtagccgac aatacggaaa cggtgagtca
     6601 cgccatcgtc gttttcaatc tccacccacg cgccaaaaaa gactttgcct tcctgctgag
     6661 gggagtaatt gacgattttg agattttcca tgcatttagt gagatagcgc acgcgacggt
     6721 cgatttcacg cagacgcttt ttattatact gatagtcagc attttcgctg cggtcgccca
     6781 gacttgcggc ccaggtcacc ttttttgtga cctccgggcg ttcttcacgc cagagataat
     6841 taagctcttg tttgagtttt tcataccctt cccgggtaac caggggcgtt ttcatctcgt
     6901 tgattccctt tgtctgtttg ataatgcgca cattgggtat aacgtgatca tatcaacaga
     6961 atcaataatg tttcgccgaa taaattgtat acttaagctg ctgtttaata tgctttgtaa
     7021 caatttaggc tgaaattcat accagattta gctggtgacg aacgtgagct tttttaagaa
     7081 tacacgctta caaattgttg cgaacctttg ggagtacaaa caatgcaaga gaactacaag
     7141 attctggtgg tcgatgacga catgcgcctg cgtgcgctgc tggaacgtta tctcaccgaa
     7201 caaggcttcc aggttcgaag cgtcgctaat gcagaacaga tggatcgcct gctgactcgt
     7261 gaatctttcc atcttatggt actggattta atgttacctg gtgaagatgg cttgtcgatt
     7321 tgccgacgtc ttcgtagtca gagcaacccg atgccgatca ttatggtgac ggcgaaaggg
     7381 gaagaagtgg accgtatcgt aggcctggag attggcgctg acgactacat tccaaaaccg
     7441 tttaacccgc gtgaactgct ggcccgtatt cgtgcggtgc tgcgtcgtca ggcgaacgaa
     7501 ctgccaggcg caccgtcaca ggaagaggcg gtaattgctt tcggtaagtt caaacttaac
     7561 ctcggtacgc gcgaaatgtt ccgcgaagat gagccgatgc cgctcaccag cggtgagttt
     7621 gcggtactga aggcactggt cagccatccg cgtgagccgc tctcccgcga taagctgatg
     7681 aaccttgccc gtggtcgtga atattccgca atggaacgct ccatcgacgt gcagatttcg
     7741 cgtctgcgcc gcatggtaga agaagatcca gcgcatccgc gttacattca gaccgtttgg
     7801 ggcctgggct acgtctttgt accggacggc tctaaagcat gaggcgattg cgcttctcgc
     7861 cacgaagttc atttgcccgt acgttattgc tcatcgtcac cttgctgttc gccagcctgg
     7921 tgacgactta tctggtggtg ctgaacttcg cgattttgcc gagcctccag cagtttaata
     7981 aagtcctcgc gtacgaagtg cgtatgttga tgaccgacaa actgcaactg gaggacggca
     8041 cgcagttggt tgtgcctccc gctttccgtc gggagatcta ccgtgagctg gggatctctc
     8101 tctactccaa cgaggctgcc gaagaggcag gtctgcgttg ggcgcaacac tatgaattct
     8161 taagccatca gatggcacag caactgggcg gcccgacgga agtgcgcgtt gaggtcaaca
     8221 aaagttcgcc tgtcgtctgg ctgaaaacct ggctgtcgcc caatatctgg gtacgcgtgc
     8281 cgctgaccga aattcatcag ggcgatttct ctccgctgtt ccgctatacg ctggcgatta
     8341 tgctattggc gataggcggg gcgtggctgt ttattcgtat ccagaaccga ccgttggtcg
     8401 atctcgaaca tgctgctttg caggtcggta aagggattat tccgccgccg ctgcgtgagt
     8461 atggcgcgtc cgaggtgcgt tccgttaccc gcgcctttaa ccatatggcg gctggtgtta
     8521 agcaactggc ggatgaccgc acgctgctga tggcgggggt aagtcacgac ttgcgcacgc
     8581 cgctgacgcg tattcgcctg gcgactgaga tgatgagcga gcaggatggc tatctggcag
     8641 aatcgatcaa taaagatatc gaagagtgca acgccatcat tgagcagttt atcgactacc
     8701 tgcgcaccgg gcaggagatg ccgatggaaa tggcggatct taatgcagtg ctcggtgagg
     8761 tgattgctgc cgaaagtggc tatgagcggg aaattgaaac cgcgctttac cccggcagca
     8821 ttgaagtgaa aatgcacccg ctgtcgatca aacgcgcggt ggcgaatatg gtggtcaacg
     8881 ccgctcgtta tggcaatggc tggatcaaag tcagcagcgg aacggagccg aatcgcgcct
     8941 ggttccaggt ggaagatgac ggtccgggaa ttgcgccgga acaacgtaag cacctgttcc
     9001 agccgtttgt ccgcggcgac agtgcgcgca ccattagcgg cacgggatta gggctggcaa
     9061 ttgtgcagcg tatcgtggat aaccataacg ggatgctgga gcttggcacc agcgagcggg
     9121 gcgggctttc cattcgcgcc tggctgccag tgccggtaac gcgggcgcag ggcacgacaa
     9181 aagaagggta aataaacggg aggcgaaggt gcctcccgtt ttgctttcta taagatactg
     9241 gatagatatt ctccagcttc aaatcattac agtttcggac cagccgctac cagcgcggca
     9301 cccgcagggg tgtcggtgta tttatcgaag ttgtcgataa acagtttcgc cagggtttcg
     9361 gctttttcct gccactgttc cggagaagcg taggtgttac gcggatcgag aatcttcgtg
     9421 tctacgcccg gcagttcggt tgggatcgcc aggttaaaca tcggcagagt gaaggtttct
     9481 gcattatcca gcgaaccgtt gaggatggcg tcgataatgg cgcgggtatc tttaatcgag
     9541 atacgtttgc cagtgccgtt ccagccagtg ttaaccagat aagcctgcgc gcccgccgcc
     9601 tgcatacgtt tcaccagcac ttctgcgtac tgagtcgggt gcagcgacag gaacgccgcg
     9661 ccgaagcaag cggagaaggt tggcgtcggt tcggtgatgc cacgctcagt accggccagt
     9721 ttggcggtga agccagagag gaagtgatac tgggtttgat cggcagtcag gcgagaaacc
     9781 ggcggcaaca cgccgaaagc atcagcagtc aggaagataa ccttagtcgc gtggcccgct
     9841 ttggaaaccg gcttaacgat gttatcgatg tgatagattg gataagaaac gcgggtgttc
     9901 tcggtttttg aaccatcatc aaagtcgata gtgccatctt cacgcacggt gacgttttcc
     9961 agcagcgcat cacgacggat agcgttgtag atttcaggtt ccgcttcttt cgacagcttg
    10021 atagtttttg cgtagcagcc gccttcgaaa ttaaacacgc cgtcatcgtc ccagccgtgt
    10081 tcgtcatcgc caatcaggcg gcgtttcggg tcggtggaaa gggtggtttt accggtgccg
    10141 gaaaggccga agaacaccgc aacatcgcct ttctcgccaa cgttggcgga gcagtgcata
    10201 gaagcgatac ctttcagcgg cagcaggtag ttcatcatcg agaacatccc tttcttcatt
    10261 tcgccgccgt accaggtgcc gccaatcagc tgcatgcgct cggtcaggtt aaacgccacg
    10321 aagttttcgg agttcagacc ctgttctttc cactgcgggt tagtgcactt cgcgccgttc
    10381 ataacgataa agtctggttt gaaacctgcc agctcttcat cgctcgggcg aataaacatg
    10441 tttttgacaa aatgcgcctg ccaggccact tcggtgatga aacggacgga aagacgagta
    10501 tccgggttcg caccacagaa agcgtcgaca acgaacagac gtttgccgga aagctgcctg
    10561 gtcaccaggc ctttcagatg ctgccaggtt tccggagaga gaggtttgtt gtcgttctta
    10621 cctttgcctt tgtctgccca ccagaaagta tcgcgagtgg tatcgtcacg gacgatatac
    10681 ttatcttttg gtgaacgacc ggtgaagatc ccggtatcga cggcaacggc acccagatta
    10741 gttaacaccc cgcgctcata acctgtcagg ctcggatcga gctcttcctg atacagcagg
    10801 tcgtagcttg ggttgtaaac gatatcatgt acgtcactga taccataagc ctcgagttct
    10861 tgcggggtca aaccattgtt aacgcgcatt tcactgctcc ttagccaata tgtattgcct
    10921 gaatagtaaa gtctttttgg gggtgttaac cgcgacaagg ctcatagatt tacgtatctg
    10981 gagaaattca tatttgacaa aaaactccgt gattcctgtc acgaaacggt tgctattatc
    11041 gcagaaatag cattttaggt gtggaaaatg ttctcaaaag gttaaatctt ggtttatatg
    11101 gcgggaatat tgtgagtgga atcgcattcc tgaatgaaat tgatgcaaat tcaaccggta
    11161 aagtatcgat tcaccacgct aacttttttt gattattttc tgaaaaaagc aaaatactac
    11221 tgcccggatg cgataattaa cccacctttt gattctggat aaccaacatg gataacgttg
    11281 aactttcacc ggcaacacgc tgggggatga ttgctaccgg attacttcag gggctggttt
    11341 gctacctgct tatcgcctgg cttgccggaa aaaatcacag ttggattgtt tatggcgtgc
    11401 ctgcgaccgt cgcgttttca tccgttttac tcttctccgt gatctctttt aaacagaagc
    11461 gcctttgggg atggctggcg ctggtgttta ttgccacgac agggatgagc ggttggctga
    11521 agtggcaaac tgatggcatg aacccctgga gagctgaaaa ggcgatttgg gattttggct
    11581 gatatctgct gttgatggca atgttgttgc taccgtggat acaacaaagc cttcgtatcc
    11641 gcaatggcag tagccgctat agctactttt accagtcagt atggcataac gtacttatat
    11701 tactggtgat ttttctctcc aatggcctga cgtggctggt gcttttactg tggggtgagt
    11761 tgtttaaact tgttggcatc acgtttttca atacgctctt ttttgcaacc gactggttta
    11821 tgtatcttac atttggtctg gttaccgcac tggtggtgat cctcgcgcgt acacagtcac
    11881 gtttaatcga ctctattcaa aagttgttca cgctaatcgc cacggggttg ctgccgttag
    11941 tatcattgct aaccctgatg tttatcatca ccctgccgtt tacggacctg agcgcgattt
    12001 ctcgccacat ctccgccgcc gggttgctgt taacgctggc ctttttgcaa ttgatcttaa
    12061 tggctattgt ccgcgatccg caaaaagcgt cacttccctg gacggggccg ttgcgttgcc
    12121 tgattaaaac cgctttgctg gttgctccgc tgtatgtgtt cgtcgccgcc tgggcgttat
    12181 ggctgcgggt cgctcagtac ggctggactg tcgaccgctt gcagggcgcg ctggcggtgc
    12241 tggtgttact ggtgtggtcg ctggggtatt ttgtcagtat cgtctggcgt aatgggcaaa
    12301 atccccttgt tcttcagggc aaagtgaacc ttgcggtttc gttattggtg ttggtgatac
    12361 tggtgcttct taattcgccg gtgctggaca gtatgcgcat tagcgtgaac agtcatatgg
    12421 cgcgttatca gagcggcaaa aacacgccag accaggtaac tatctacatg ctcgaacaga
    12481 gcggtcgcta tggacgtgcg gcgcttgagt cgctgaaaag cgatgccggg tttatgaaag
    12541 acccgaaacg cgcgcgggat ctgctgatgg cgttagatgg agagcaacat cttcaggaac
    12601 aggtatcgga aaaagtatta gccgaaaatg tgttaattgc tcctggttct gttaaaccgg
    12661 atgcgacatt ctggtcggcc ttaatccagg atcgctataa cgtgatgacc tgtattgaaa
    12721 aagacgcctg cgtcctggtc gagcaagatc tgaatagtga tggtcaggcg gagcggatcc
    12781 tgtttgcttt taatgatgac agagtcattg tctatggctt tgactcagac agaaaagaat
    12841 gggacgcgct tgatatgagt ttacttccga acgaaataac gaaagaaaaa ttactcacag
    12901 ctgcgaagga tggaaaactg gatctgacgg tagatggtga aacgctggaa ataaacttaa
    12961 gtaagtgaca actcttttaa cggatacgac gctgtatctg accaacaata atttgcgatt
    13021 tatttcacag gcctggtaag cgcagcacat caggccatta cgtcgtaaaa tccggcagag
    13081 ccctctctgc cggacatact cattaatgaa cttgcggatc tgccggagac gcgttgttgc
    13141 ggatttcagc aatatccatc gcattgaaca gatagtggtt accgcagtaa tcacaatgca
    13201 tgtcaatttc gccatcttcc gccaggatgc tatcgacttc ttcatcaggc agcgttttca
    13261 gcgcgtcggc gcaacgttca cgcgagcagg tgcatttgaa ctccacatcc tgcggatcgt
    13321 aaaccgtcac ctcttcttcg tgatacaaac gccacaacac ttcgtttgcc ggtaaggtca
    13381 gcagttcttc ggttttgatg gtttcggtta gcgtcgccag gtggtcaaag tcgtcctgct
    13441 gggcattttg cgcaggcatt acctgcaaca acataccgcc tgcagccggt ttgccgtcta
    13501 cgtcgccggt gcgaataaac aggcgcgtcg gcagctgttc agaacgcata aagtaatctt
    13561 ccaggcaggc cgccagggta tcaccttcca gaccaaccac gccctgatag cgttcgcctt
    13621 cgctcggggt aatggtgatc accacgtaac cattgccgac cagcgttttc aggtcggcat
    13681 tttctggaat ttcgccctgc acgcgcgcca caccgcgcat ttgctggtta ttgttaccgt
    13741 taataaccgc cagattcatc ggaccgtcgc cttgcagctg tacggtgata tcaccatcaa
    13801 acttcagcgt agcggttaac aggctggtcg caaccagcag ttctgccagc acgtttttaa
    13861 cgggctgcgg ataatcgtga ttctcaagga tctgttgcag ggtttccgaa acggttacca
    13921 gttcgccgcg cacggcaaag ttttcaaaca gatagcgatg taattggtca tgttgcggca
    13981 taatcatctc tcttgcaggt gacagttatt cactgtcgcc gtgtttaaat cgtaacaggt
    14041 cgcggcgctc ttttttgtcc gggcgtcggt ccgggtgcgg catggttaag gcattaagtt
    14101 tacgtgccag cgccattttt tcgcgtttct ctacactttc cgcagtctct tcatacagca
    14161 aggctgcctc gctggcgggg cgacgctgtt cagtaatcgc ctttacaatc accgtgcgtt
    14221 cgtcatttcc ctggcgcaga gtgagcgtgg cattcagctc gacgattttg ctcggcttgc
    14281 tgcgctgccc gttgtaatgc accttaccgc cttcaatcat ttcacgggcc agcgcgcggg
    14341 ttttataaaa acgggcagcc catagccatt tatccagtcg aacctcaaca gcaggttttt
    14401 ctttcatggc gtctccttca cattagcgag gggatcaggc ggcggtagtc attcagtgac
    14461 ggatggcgtt gatactgttt ctcggcaatc ccggaatcag gattagtcac gccgaggcag
    14521 taacgaatac caaattgcgc ggcagcatcg agaatcgctt cgctgtcatc aataaacagc
    14581 gttctttcag ctttcagacc cgtagcttcg gccaccgcat gccataaccg ctgatcctct
    14641 ttcggataac caaatgtgtg ggtggaaagt aataaatcaa ggtgtgcgtc cagaccggta
    14701 tgctcaagtt ttaccgccag gttgtgcgga tgcgcattgg tgagcaaaat tcgctgctta
    14761 ccgctggctt tcagtgcctc aagaaacgga atggtatctt cacgcagtac ggcacgcggt
    14821 cccatctcgg tggtcatcgc acagatatcc agacccagtt gctcactcca gtaatcaaga
    14881 cagtaccagt ttagcgtatg ctgtacgtcg tgatattgct ggcgcatata ttccatcgct
    14941 tcctgtggcg taaccccgtt tttcgcgccc catgtttcag gcaccagctt ttgccagaaa
    15001 tagttatcga aggcgaggtc gagcaacgtg ccgtccatat ccagcagaac ggtatctacg
    15061 tcctgccagg caatgttgat atgcatgagg gaaatctcca gagtgaagca atttgcgcga
    15121 cagggtagca taacctgccg cgcaaacgtg ttattcgata aggctttctg aaggggtgat
    15181 cagttgcggg ttcaggcagc tttcataata ggcctgaatc ttcatcatgc gagtgcgatg
    15241 acgctggtaa cgacgccagg cctgtacgcc attgtaaatg gcgctaccca gcatcgtcag
    15301 tagcagcaat gtggtgctga gatagcgcca caggccggaa cgatccggga ttggatgaag
    15361 gccaatatgc tgcgtaccat tggcgtcagt gaagattttt gtgacgatac cctcggcgtt
    15421 aaacggcgta tgcatcagca tttgtgccag tttctggaaa gcgttccact gttcttgcgg
    15481 cgggtagtcg taaagtgatg ccgaaggcca gggctgatca acaaaatcgc tgccttcgtc
    15541 gctgacaatc aggaatccgc cgggggccgg actgtttagt gattgtgccg ctcgcgccgt
    15601 ttcatgcgtg ataaacggcg cggtggaggt tgccaccagg ttatccagcg attccgcact
    15661 caccgggcgt aataacacat tcacgccatc gagcttcccg gcgttggcgc gttttaccag
    15721 cgcgtcccag tctttactgt tgccgagatt gaccagcgca tttttcagtc gcacacagtc
    15781 atctttggca gaacataaat ccgctgtctt cagtacaatg tcgccaaaat catcaagcaa
    15841 taccatgccg gatttttgaa ttgctgaacg taatgaggca ctgacgcgag atccatcttc
    15901 cggtttaggg tgcagctggc gattaactgc ttcagtcaat gccgtcgctt tgttgaccag
    15961 ttcagattct ggtaatggca atgagcgggc gtcgttccag atgatctgcg agcagtcaaa
    16021 cggtaaaaaa ggtgaattgg ttttcgcgct ccaggttccg gaagtgcgaa tattacacat
    16081 tcccgtaccg ctgatacgca atgtatcgcc tacccgcacg ccagcgtcag ccagttgttt
    16141 tacgctggtg gcttcaatgg tctgtgcgcc tttcatccat gagagagtga atttcagcgg
    16201 catatccagc gggatccaga ataacagcat aaacagcacc agcagcgagc ccgcagcgat
    16261 aatcgtactg cgcagccagt gctgtaacgg aaagtttttt acttcatcat gcagcgaaag
    16321 atatcgcccc tgacgcacta cgtggcggtc gagatagata tcaatatcgg tttgttgacc
    16381 gagatcctga gcaatgtatg gctgccagtg tgcgggatag accaggtcga taataccgag
    16441 tgaaatattg ttgatctgtt cctgatcgtt ttcgccaaac aatccccaac gacggggtgt
    16501 accgcgcaga caatgaattt cccgcaggga ggattttgcc gggggcgcga atagccccca
    16561 cagacctgcg cccagcagca gtaatgcgcc gcctgccagc cacggaacaa atacatccgg
    16621 ggtaatcagg cagaaaaaga acatcaggaa ggaggcgacg atcagcaacg cttcacgcag
    16681 cccgcgcgga cgactcagcg catattcttc atgcgtttct ttgcgaatat tgagtagctc
    16741 gatttgctca ctctcttccc cgcgaatcga cgcctgcgtg gacggaacag gttgcaaggc
    16801 atagccgcgc gtttcctgca tgtactcctg cagcgtatga ccgttgagtg aaataaccag
    16861 cggcagcgaa tcggtataaa tcagttcaac ggtattctca tcgttgatgt attgttccca
    16921 gaaagggggc aggtggactt ctaccgaatc gaggtagtaa cgccatttat tcggatcgtc
    16981 ggtagagatg ccgtaacgcg tgatagcgtg tgtcagcatc atgacgttgt tgctttcggc
    17041 attcagcgcc agagagatcg gtgccgcgct agctcccgtt gggccaggca cctgtaatac
    17101 ctgcgtcagg ctctcaagat aattttcaac ggcgctacgt tcttcaggtg tgagtttacg
    17161 cgtttgcgca tccgcgaagg cgttggtcca gggcagctga cgccgtctgg atcgcacttt
    17221 tatcagccat cccgcaagta gtgagcaggc cagcaaagca gctaaaaaaa tcacaatggt
    17281 gctcatgctt tccccatctt acttagtctg tcaggcgtgg tcagtcgcac cagacacaat
    17341 acatgattct gcggttggct atcggcaagc gtggagttta acttgagcat cattaagcac
    17401 atcacagtaa ccgctgatca tagcaaaccc tctgaaggcg ggatatcagc aaattcctgg
    17461 agttatttca acctattgac ttttaatttg atttgaggac gattatttat taagttacat
    17521 aaatgtaaat aacatgcaat tcacattgcc gaaagcgtgt ccgatatcgc acaataaccg
    17581 ctgatttcac agcatagatc ccacgatgag caaatcatta caaaaaccca ccattctgaa
    17641 tgttgaaact gtagcccatt cccgactgtt taccgtcgag agcgtggatc tggagttcag
    17701 caatggcgtg cggcgtgttt atgaacgaat gcgtccaacc aaccgggaag cagtgatgat
    17761 tgtgccgatt gtggacgatc acctgatcct gatccgcgaa tacgcagtgg gaactgaatc
    17821 ctacgaatta ggtttttcga aaggattaat tgatccgggt gaaagcgtct acgaagccgc
    17881 taaccgcgag ctaaaagaag aggttggatt tggtgcgaac gatctgactt ttttgaagaa
    17941 gctcagcatg gcaccgtctt acttttccag caaaatgaat atcgtggtcg cgcaagatct
    18001 ctacccggaa tcgctggaag gcgatgagcc agagccgcta ccacaggtgc gctggccgct
    18061 ggcgcatatg atggatttgc tggaagaccc tgacttcaac gaagcgcgta atgtcagcgc
    18121 gctgttcctc gtgcgcgaat ggttgaaagg gcaggggcga gtgtaaatgc gtgaagtttg
    18181 cctgatgcac aacgattatc aggcttatgg ggttcctgca atatattgat ttttcacgat
    18241 tttgttagcc ggataacgcg ttcacccgca tccggcataa acaaatgcac tttgtcagcc
    18301 aactgaaaag gcgccgaagc gcctttttaa tcagaacaat tcctgtgcct caccattatc
    18361 gataatggtt gttcccacct cgtgcaccgc ctgttgtgtc ggctgcgtac cttcgatgaa
    18421 atactcttcg cggctgttgc caccattagc taactgcccg gtgctgcgat cgatattcac
    18481 cgtcacaata cccggaggcg gcgtcagcgg ctgctccggc acaccttcaa gaacggcttt
    18541 catataagcg tcccatgcag gctgggcact cttggcaccg ccttcgtaac ctgagatctg
    18601 atctttaatc gctccggaag ccgttgtatg accgagatta cgacggtgat catcaaagcc
    18661 aatccagacc gaggtcacaa cgcccggacc gtaacccgag aaccacgcat ctttcgaact
    18721 gttagtggta ccggttttcc cgccgatatc gcgacgctgc aaatcacgac ctgcacgcca
    18781 gccggtaccc tgccagcctg gttcaccaaa gatattggtg ttcaaagcac tcttaatcag
    18841 gaatgccagc ggagtgttga tgacgtgcgg tgcgtactcc tgcgcgccag tcttcgccac
    18901 taacgcctga tttgcctgct ccagctgcgg cattggtaca gaaacattct gctgctcgcg
    18961 ggagatagcg acatcttcaa catcgttatt ttccagcacg ttcgatttct gcgtatcacc
    19021 gtaaatcacc ggaatatcgc attccgggca ggctactttc ggtttcgctt cgaaaatcac
    19081 gccgccctga tcgttttcaa ttttgctgat aaaccacggg tccaccagga agccgccgtt
    19141 cgccatgacc gcgtagccgc gcgccacctg cattggggtg aaggacgctg aacccagcgc
    19201 cagcgattcg gtgtggacaa tgttttgcgc cgggaagccg aagcgttgca gatattctgc
    19261 agcgtagtcg acgcccatcg cccgcattgc acgtaccatc accacgtttt tcgactgacc
    19321 cagcccctga cgtaagcgaa ttggaccagc atactgcggt ggtgagttct tcggctgcca
    19381 gtcagaaccg acacctgcat cccagcgaga aattggcaca tcgttcaaca tacttgccag
    19441 cgtcagacct ttatccatcg ccgcggtgta gaggaacggt ttgatgttgg aacccacctg
    19501 acgcagtgcc tgggtggcgc ggttaaactt gctctgattg aaatcaaagc caccgaccag
    19561 cgccataacg gcaccatttt gcggattgat cgacaccagc gccgagttca cttccggcac
    19621 ttgtgccagc caccatgcat cgccaacctg acgaacccag atttgctgac ccgtttgcag
    19681 aacatcggtc actttacgcg gcgtcggtcc ctgctgagta tccgaacggt aaggacgcgc
    19741 ccagcgaacg ccgtccatac tcaatgcgac agtcgacccg tccgccagca ttgccgtcgc
    19801 ttcctgagga ttggcgctgg tgactgcggc aggcagcagc ggaccatagg tcggcagcgc
    19861 cttcagcgta tcggtaatct tgttgttatc ccacgccgac tcgcccactt tccacagcac
    19921 atttgccggg ccgcgatagc cgtggcgcat gtcgtagtcc agcacgttat tacgtaccgc
    19981 ctgctgtgcg gcctgctgca ctttgcgggt gatggtggtg taaatgcgat aaccgtcttc
    20041 ataggcactt tcgccataac ggttatacat ctcctggcgc accatttcgc tcaggtacgg
    20101 cgcagagaaa gcaatctccg gcgcgtgata gttagcgtta atcgcctcag tgcgtgtctg
    20161 atcgaactgt tgttgggtga tatacccttc atccagcatc cgcgacagta cgacgttacg
    20221 ccgcgcgacg gcacgatcca ttgagtagag cggattaaag gtggaaggcg ctttcggcag
    20281 cccggctatc acagccattt cgttcagcgt cagttggtcg accgttttac cgaaatagac
    20341 ttgtgccgca gcaccgacac cataggcgcg gtaaccgagg taaatcttgt tcagataaag
    20401 ctcgaggatc tcgtctttcg tcagcaactg ttcaatgcga atcgcgagga agacttcctt
    20461 aatcttacgc atcagcgtgc gttctggact gaggaagaag tttctcgcca gctgctgggt
    20521 aatggtactc gccccttgtg acgcgtggcc ggagaacagc gccacacttg ccgcacggaa
    20581 gatccccacc gggtcaacgc cgtgatgctc gtagaagcgg ctgtcttccg tcgcgataaa
    20641 ggctttcacc atctccggcg ggatttgatc caacgtaacc ggaatacgac gtttctcacc
    20701 gtattgagca atcagctcgc catcggcgct gtaaatctgc atcggaattt gcaggcgaac
    20761 atcttttaat gtcgccacat ccggcagttg tggctcgatg tagcggtata ggccataaat
    20821 cgagcctgct cccagcagaa tgcaacagac tgcaaggatc aaaaaatact ttacgaactt
    20881 cactggaaat ttcccattta gtttcatttg ggcagtttat aaacaaacgt gcggtagtat
    20941 aaaggcaagc cagacgcatt gatatacccg tcagagtgac gggtgataag gagatcatca
    21001 caatggcatt taagatctgg caaattggtt tgcatttaca acagcaagaa gcagtagcgg
    21061 ttgcgatcgt gcggggcgca aaagaatgct ttttgcaacg ctggtggcgg ttgccgctgg
    21121 agaacgacat tatcaaagat gggcggattg ttgatgcgca gcggctggct aaaacgttgt
    21181 taccgtggag tcgcgaactg ccgcagcgtc atcacattat gttggcgttt cccgccagtc
    21241 gcacattaca gcggtcattt ccgcgcccgt cgatgtccct cggtgagcgg gagcaaacgg
    21301 cctggctgtc agggacgatg gcccgcgagc tggatatgga tccggactcc ctgcgtttcg
    21361 attacagcga agactcactc agtcccgcct ataacgtgac tgccgcgcaa agcaaagagc
    21421 tggcaacgct actcactctg gcagaaaggt tgcgtgttca tgtgagcgcg atcaccccgg
    21481 atgccagtgc attacagcga ttcctgcctt ttttaccttc tcatcagcaa tgtctggcct
    21541 ggcgtgataa cgaacagtgg ctgtgggcga cacgctgtcg ctgggggcgc aaactggcgg
    21601 tggggatgac tagcgcgaag gagctggcgg cagcgttatc cgttgatccc gaaagcgtcg
    21661 cgatatgtgg cgaaggcgga tttgatccct gggaggccgt ttctgttcgt cagccgccgc
    21721 taccgccgcc aggtggagac tttgccatcg cgctggggct ggcgcttggg aaggcgtact
    21781 gatgaacccg ccaattaatt ttttgccctg gcgacagcaa cgccggaccg cttttcttcg
    21841 tttctggttg ctgatgttcg ttgcgcctct gctgctggcc gtcgggataa cgctaatact
    21901 gcgtctgaca gacaacgccg aagcacgcgt aaacaccgtt ttgcttcagg cggaacaaca
    21961 actcgcccgc agcttacaga taacgaaacc acgtttgctg gagcgccaac aaattcgtga
    22021 gcagcgtttg caacggcagc gccagcgaca atttacccgc gactggcaat ctgcgctgga
    22081 agcactggcg gcgcttttac ccgagcacgc ctagctgaca acgataagct ggcagcaggg
    22141 aacgctggag atcaaggggc tgacaacaag cattaccgcg ttaaacgcac tagaaacgtc
    22201 actccgccag gatgcgtctt ttcatctcaa tcagcgggga gccacgcagc aggatgcgca
    22261 gggacgctgg caatttgagt atcagttaac aaggaaggtt agcgatgaac atgttctttg
    22321 actggtggtt cgccacatca ccccgcctcc gccagttttg ctgggcattc tggttgctga
    22381 tgttagttac gctcattttt ctgtcatcga cacaccatga agagcgcgac gcattaattc
    22441 gactacgggc aagtcatcac cagcagtggg ccacactgta tcgcctggtt gacaccactc
    22501 ccttcagcga agaaaaaacg ctgccctttt cgccactgga ttttcagttg cccggcgcgc
    22561 aactggtttc ctggcatcca tccgcgcagg gaggcgagtt ggcgttgaaa acgctgtggg
    22621 aagcagtgcc gtcggcattt acacggctgg cagagcgcaa cgtcagcgtg agccgttttt
    22681 cattaagcgt ggaaggtgat gatcttttgt tcacgctaca actggagacg ccgcatgagg
    22741 gttaaacgct ggttgttggc aggtattgta ttgtgccttt taaccggtat gcgtgaccct
    22801 tttaaaccgc cggaagatct atgccggatt agcgaactta gccagtggcg ctatcagggg
    22861 atggtagggc gaggcgagcg catcatcggt gtaataaaag acgggcaaaa gaaatggcga
    22921 cgggtgcagc aaaacgatgt gctggaaaac ggctggacaa ttttacagct gacgccagac
    22981 gtactaacgc tgggtaccgg gacaaactgc gaaccgccac aatggttgtg gcaacggcaa
    23041 ggagatacaa atgaagcaat ggatagccgc actactgttg atgctgatac ccggcgtaca
    23101 ggcggcaaag ccgcaaaaag tgacgctgat ggtggatgac gttccggtag ctcaggtgtt
    23161 gcaggcgctg gctgaacagg agaagttgaa cctggtcgtg tcgccagacg tcagcggtac
    23221 ggtgtcgtta catctaacag atgttccctg gaagcaggca ctacaaactg tagtgaaaag
    23281 cgccggactg ataacgcggc aggaaggcaa cattctctca gtgcattcca ttgcctggca
    23341 gaataacaat atcgcccgcc aggaggcgga gcaggcgcgg gcgcaggcaa atctgccgct
    23401 ggaaaatcgc agtataaccc tgcaatacgc cgacgcggga gaactggcga aagcggggga
    23461 gaagctactg agtgccaaag ggagtatgac cgtcgataaa cgcaccaatc gccttttgct
    23521 acgagataac aaaacggcgt taagcgcgct tgaacagtgg gtagcgcaaa tggatctgcc
    23581 ggtcgggcag gttgagctgt cggcgcatat tgtcaccatt aatgaaaaaa gtttgcgtga
    23641 gttaggcgtg aaatggacgc tggccgatgc gcaacacgct ggtggcgttg ggcaagtcac
    23701 cacgcttggt agcgacctct ccgtagcgac ggcgacaacg catgtcggtt ttaacattgg
    23761 gcgcatcaac ggacgcttgc tggatcttga gctttccgcg ctcgaacaaa aacagcagct
    23821 ggatattatc gccagtccgc gtctgctggc ctcacatctt cagcctgcca gcattaaaca
    23881 ggggagcgaa attccatatc aggtttccag cggggaaagt ggcgcgacgt cggtggaatt
    23941 taaagaggcc gtcctgggga tggaagttac gcccacagtg ttacaaaaag gtcgcatccg
    24001 gctgaaatta cacatcagcc agaacgttcc ggggcaggtg ctacagcagg ccgatggcga
    24061 agtgctggcg attgataagc aggagatcga aacgcaggtc gaggtcaaaa gcggagaaac
    24121 gttggcgctg ggcggcattt ttacccgtaa aaataaatcg ggtcaggata gcgtaccgtt
    24181 gcttggcgac attccctggt tcgggcaatt atttcgtcat gacggaaaag aagatgaacg
    24241 acgcgagtta gtggtgttta tcacgccacg actggtttcc agtgagtaaa cagccgtaaa
    24301 agcggtaatg tttttacgct gaacgtgttt catctatttg acgcgcgcag gtatttagca
    24361 tacaaggagt accgatttga gagttggtgc tcttcgctgc ctgcgttcca tgatgatgat
    24421 ttatcattca ggcggcattt tgctgtcttt tttacgctaa tcttacccgg tgatttatcg
    24481 ccagagcggt ggtagcaagg cagcgcgctt gcagcgacca gatatgcaga gggatgggtg
    24541 atttattcag ttgccaaacc cgctggagta ttgagataat tttcagtctg actctcgcaa
    24601 tatcttatga ggtttcagtt catgtcctgc ggcgctctct gagcgaagcg ggtttatcat
    24661 taacgaatag tcttagtagt accgaaaaaa tggcagagaa acgcaatatc tttctggttg
    24721 ggcctatggg tgccggaaaa agcactattg ggcgccagtt agctcaacaa ctcaatatgg
    24781 aattttacga ttccgatcaa gagattgaga aacgaaccgg agctgatgtg ggctgggttt
    24841 tcgatttaga aggcgaagaa ggcttccgcg atcgcgaaga aaaggtcatc aatgagttga
    24901 ccgagaaaca gggtattgtg ctggctactg gcggtggctc tgtgaaatcc cgtgaaacgc
    24961 gtaaccgtct ttccgctcgt ggcgttgtcg tttatcttga aacgaccatc gaaaagcaac
    25021 ttgcacgcac gcagcgtgat aaaaaacgcc cgttgctgca cgttgaaaca ccgccacgtg
    25081 aagttctgga agcgttggcc aatgaacgca atccgctgta tgaagagatt gccgacgtga
    25141 ccattcgtac tgatgatcaa agcgctaaag tggttgcaaa ccagattatt cacatgctgg
    25201 aaagcaacta attctggctt tatatacact cgtctgcggg tacagtaatt aaggtggatg
    25261 tcgcgttatg gagaggattg ttgttactct cggggaacgt agttacccaa ttaccatcgc
    25321 atctggtttg tttaatgaac cagcttcatt cttaccgctg aaatcgggcg agcaggtcat
    25381 gttggtcacc aacgaaaccc tggctcctct gtatctcgat aaggtccgcg gcgtacttga
    25441 acaggcgggt gttaatgtcg atagcgttat cctccctgac ggcgagcagt ataaaagcct
    25501 ggctgtactc gataccgtct ttacggcgtt gttacaaaag ccgcatggtc gcgatactac
    25561 gctggtggcg cttggcggcg gcgtagtggg cgatctgacc ggcttcgcgg cggcgagtta
    25621 tcagcgcggt gttcgtttca ttcaagtccc gacgacgtta ctgtcgcagg tcgattcctc
    25681 cgttggcggc aaaactgcgg tcaaccatcc cctcggtaaa aacatgattg gcgcgttcta
    25741 ccagcctgct tcagtggtgg tggatctcga ctgtctgaaa acgcttcccc cgcgtgagtt
    25801 agcgtcgggg ctggcagaag tcatcaaata cggcattatt cttgacggtg cgttttttaa
    25861 ctggctggaa gagaatctgg atgcgttgtt gcgtctggac ggtccggcaa tggcgtactg
    25921 tattcgccgt tgttgtgaac tgaaggcaga agttgtcgcc gccgacgagc gcgaaaccgg
    25981 gttacgtgct ttactgaatc tgggacacac ctttggtcat gccattgaag ctgaaatggg
    26041 gtatggcaat tggttacatg gtgaagcggt cgctgcgggt atggtgatgg cggcgcggac
    26101 gtcggaacgt ctcgggcagt ttagttctgc cgaaacgcag cgtattataa ccctgctcaa
    26161 gcgggctggg ttaccggtca atgggccgcg cgaaatgtcc gcgcaggcgt atttaccgca
    26221 tatgctgcgt gacaagaaag tccttgcggg agagatacgc ttaattcttc cgttggcaat
    26281 tggtaagagt gaagttcgca gcggcgtttc gcacgagctt gttcttaacg ccattgccga
    26341 ttgtcaatca gcgtaacaac aagaaaggtc aggccgctta tcaagcggtc tattagcttc
    26401 aggttaattg caacgtggta agcattaacc ttttagtggg gtgttaaatg gatgaattca
    26461 aaccagaaga cgagctgaaa cccgatccca gcgatcgtcg tactggtcgt tctcgtcaat
    26521 cttctgaacg ttctgagcgt actgaacgtg gcgaaccgca gatcaatttt gatgatattg
    26581 aacttgatga cactgacgat cgccgtccga ctcgtgcgca aaaagagcgc aatgaggaac
    26641 cggaaatcga agaagaaatt gacgaatccg aagatgaaac cgtggatgaa gagcgcgtag
    26701 agcgtcgtcc gcgtaagcgc aaaaaagcag ccagtaaacc cgcttctcgt cagtatatga
    26761 tgatgggcgt cggcattctg gttctactgc tgttgatcat cggtatcggt tctgcgctaa
    26821 aagccccctc gacctcttcc agcgatcaaa ccgcgtctgg cgagaagagt attgatcttg
    26881 caggcaatgc gaccgatcag gcgaatgggg tgcagccagc gccgggaacc acgtctgcgg
    26941 aaaatactca gcaggatgtt tctctgccac cgatctcttc tacgccgact caagggcaaa
    27001 ccccggcggc aacggatggt caacaacgtg ttgaagtgca gggtgacctg aacaatgcgc
    27061 tgacccagcc acaaaatcag caacagttga acaatgtggc ggtcaattcc acattgccga
    27121 ccgaacccgc gactgtcgcg cctgttcgca atggcaatgc atcgcgtgac acggcgaaaa
    27181 cgcaaaccgc tgaacgtccg tccactacgc gcccagctcg tcagcaggcg gtgattgaac
    27241 cgaaaaaacc gcaagcaacc gtgaaaacgg agccgaagcc ggtagcacag acgccgaagc
    27301 gtactgaacc agctgcccct gtggcgagca cgaaggcacc gactgcgact tctacgccag
    27361 caccaaaaga gacggcgact acggctccag tacagacggc atccccggcg caaaccacgg
    27421 caacaccagc cgctggaggg aagaccgcag gtaatgttgg ttcgttgaaa tcggcaccgt
    27481 ccagccatta cactctgcag ctgagcagtt cctctaacta cgacaacctg aacggttggg
    27541 cgaagaaaga gaatctgaaa aactacgttg tctatgaaac ggcgcgtaat ggtcagccgt
    27601 ggtatgtcct ggtttctggc gtgtacgctt cgaaagaaga ggcgaaaaaa gcggtatcta
    27661 cattgccagc agatgttcag gccaaaaacc cgtgggcgaa accgctgcgt caggtacagg
    27721 ccgatctgaa gtaatcaagg ttatctcccg caatggttta tcgttgcggg agctgcctga
    27781 agcgctggat gctgtcggag ctttctccac agccggagaa ggtgtaatta gttagtcagc
    27841 atgaagaaaa atcgcgcttt tttgaagtgg gcagggggca agtatcccct gcttgatgat
    27901 attaaacggc atttgcccaa gggcgaatgt ctggttgagc cttttgtagg tgccgggtcg
    27961 gtgtttctca acaccgactt ttctcgttat atccttgccg atatcaatag cgacctgatc
    28021 agtctctata acattgtgaa gatgcgtact gatgagtacg tacaggccgc acgcgagctg
    28081 tttgttcccg aaacaaattg cgccgaggtt tactatcagt tccgcgaaga gttcaacaaa
    28141 agccaggatc cgttccgtcg ggcggtactg tttttatatt tgaaccgcta cggttacaac
    28201 ggcctgtgtc gttacaatct gcgcggtgag tttaacgtgc cgtttggccg ctacaaaaaa
    28261 acctatttcc cggaagcaga gttgtatcac ttcgctgaaa aagcgcagaa tgcctttttc
    28321 tattgtgagt cttacgccga tagcatggcg cgcgcagatg atgcatccgt cgtctattgc
    28381 gatccgcctt atgcaccgct gtctgcgacc gccaacttta cggcgtatca cacaaacagt
    28441 tttacgcttg aacaacaagc gcatctggcg gagatcgccg aaggtctggt tgagcgccat
    28501 attccagtgc tgatctccaa tcacgatacg atgttaacgc gtgagtggta tcagcgcgca
    28561 aaattgcatg tcgtcaaagt tcgacgcagt ataagcagca acggcggcac acgtaaaaag
    28621 gtggacgaac tgctggcttt gtacaaacca ggagtcgttt cacccgcgaa aaaataattc
    28681 tcaaggagaa gcggatgaaa cagtatttga ttgccccctc aattctgtcg gctgattttg
    28741 cccgcctggg tgaagatacc gcaaaagccc tggcagctgg cgctgatgtc gtgcattttg
    28801 acgtcatgga taaccactat gttcccaatc tgacgattgg gccaatggtg ctgaaatcct
    28861 tgcgtaacta tggcattacc gcccctatcg acgtacatct gatggtgaaa cccgtcgatc
    28921 gcattgtgcc tgatttcgct gccgctggtg ccagcatcat tacctttcat ccagaagcct
    28981 ccgagcatgt tgaccgcacg ctgcaactga ttaaagaaaa tggctgtaaa gcgggtctgg
    29041 tatttaaccc ggcgacacct ctgagctatc tggattacgt gatggataag ctggatgtga
    29101 tcctgctgat gtccgtcaat cctggtttcg gcggtcagtc tttcattcct caaacactgg
    29161 ataaactgcg cgaagtacgt cgccgtatcg acgagtctgg ctttgacatt cgtctggaag
    29221 tggacggtgg cgtgaaggtg aacaacattg gcgaaatcgc tgcggcgggc gcggatatgt
    29281 tcgtcgccgg ttcggcaatc ttcgaccagc cagactacaa aaaagtcatt gatgaaatgc
    29341 gcagtgaact ggcaaaggta agtcatgaat aagtttgaag atattcgcgg cgtcgctttt
    29401 gatcttgatg gtacgctggt cgacagtgct cccggtcttg ctgctgcggt agatatggcg
    29461 ctgtatgcgc tggagttgcc catcgcaggt gaagaacgcg ttattacctg gattggtaac
    29521 ggcgcagatg ttctgatgga gcgcgcattg acctgggcgc gtcaggaacg tgcgactcag
    29581 cgtaaaacaa tgggtaaacc gcccgttgat gacgacattc cggcagaaga acaggtacgt
    29641 attctgcgta aactgttcga tcgctactat ggcgaggttg ccgaagaggg gacgtttttg
    29701 ttcccgcacg ttgccgatac gttgggcgcg ttgcaggcta aaggcctgcc gctaggcctg
    29761 gtcaccaaca aaccgacgcc gttcgtcgca ccgctgctcg aagccttaga tattgccaaa
    29821 tactttagcg tggttatcgg cggcgatgat gtgcaaaaca aaaaaccgca tccggacccg
    29881 ctgttactgg tggctgagcg gatgggaatt gccccacaac agatgctgtt tgtcggcgac
    29941 tcacgcaatg atattcaggc ggcaaaagcg gcaggttgcc catcagttgg cttaacctac
    30001 ggatataact acggcgaggc tatcgatctc agccagcctg atgtaattta tcagtctata
    30061 aatgaccttc tgcccgcatt agggcttccg catagcgaaa atcaggaatc gaaaaatgac
    30121 taagcccatc gtttttagtg gcgcacagcc ctcaggtgaa ttgaccattg gtaactacat
    30181 gggtgcgctg cgtcagtggg taaacatgca ggatgactac cattgcattt actgtatcgt
    30241 tgaccaacac gcgatcaccg tgcgccagga tgcacagaag ctgcgtaaag cgacgctgga
    30301 tacgctggcc ttgtatctgg cttgtggtat cgatcctgag aaaagcacca tttttgttca
    30361 gtcccacgta ccagaacatg cgcagttagg ctgggcactg aactgttata cctacttcgg
    30421 cgaactgagt cgcatgacgc agtttaaaga taaatctgcg cgttatgccg agaacatcaa
    30481 cgctggtctg tttgactatc cggtgctgat ggctgcggac atcctgctgt atcaaactaa
    30541 tctggtaccg gtgggtgaag accagaaaca gcacctggaa ctgagtcgcg atatcgccca
    30601 gcgtttcaac gcgctgtatg gcgagatctt taaggtgccg gagccgttta ttccgaaatc
    30661 tggcgcgcgc gtaatgtcgc tgctggagcc gaccaagaag atgtccaagt ctgacgataa
    30721 ccgcaataac gttatcggcc tgctggaaga tccgaaatcg gtagtgaaga aaatcaaacg
    30781 cgcggtcact gactccgacg agccgccggt agttcgctac gatgtgcaga acaaagcggg
    30841 cgtttccaac ctgctggata tcctttctgc ggtaacgggc cagagcatcc cagaactgga
    30901 aaaacagttc gaaggcaaga tgtatggtca tctgaaaggt gaagtggctg atgccgtttc
    30961 cggtatgctg actgaattgc aggaacgcta tcaccgtttc cgcaacgatg aagccttcct
    31021 gcaacaggtg atgaaagacg gcgcagaaaa agccagtgta cacgcttccc gtacgctgaa
    31081 agcggtgtac gaagcgattg gttttgtggc gaagccgtaa tttaacatta atgccaaaaa
    31141 ccggggaaac ccggtttttt tatcctcatt tgcaataatc cgaaaaaatg tgaagcgcct
    31201 cgccgtttcc acatctcgtc gttgccactt atcttcattt taatgaagat ataaatattc
    31261 attttattga aaatatatta tgcgtcgaac gtttatcaaa aaagaaggcg tcgtcatcac
    31321 gacgctggcc cgttatttgt tgggtgaaaa atgcggtaat cgactgaaaa ccatagatga
    31381 gctggcaact gaatgccgtt catcggttgg cctgacgcag gccgcgttga aaacgttgga
    31441 atcaagcgga gcgatacgga ttgaacgccg tgggcgcaat ggcagttatc tggtcgagat
    31501 ggataacaaa gcattgctga gtcatgtgga tatcaacaac gtggtatgtg cgatgccttt
    31561 gccctatacc cgcttgtacg aaggtttagc gagcgggctg aaagctcagt ttgatggcat
    31621 tcctttttac tatgcgcata tgcgcggcgc ggatattcgc gtggagtgtt tacttaacgg
    31681 cgtgtatgac atggcggtgg tttcccgact ggcggcggaa agttatctca cgcaaaaagg
    31741 gttatgcctc gcgctggagt tggggccgca cacctacgtt ggcgagcacc agttgatttg
    31801 ccgtaaaggc gagtccgcaa acgtgaagcg cgtggggctg gataaccgtt cggcggatca
    31861 gaaaatcatg accgatgtct tttttggcga tagtgatgtg gaacgtgtcg atctctctta
    31921 tcacgagagt ttacaacgca ttgttaaagg cgatgtcgat gcggttatct ggaacgtggt
    31981 ggcggaaaac gaactgacca tgctgggatt agaggcgacg ccgctcacag acgatccgcg
    32041 atttttacag gccaccgaag ccgtggtcct gacgcgagtc gatgattacc caatgcaaca
    32101 actgctgcgt gccgttgtag ataaacacgc cctgctcgcc catcaacaac gggtagtgag
    32161 tggggaacag gaaccgagtt attaaacgaa aggcatctga tatggaaacc agactcaacc
    32221 tgctttgcga ggcaggcgtt attgataagg acatctgcaa aggcatgatg caggtcgtca
    32281 acgtactgga aacagagtgc catctgccgg tgcgcagtga gcaaggaacg atggcgatga
    32341 cacatatggc gagtgcactg atgcgcagtc gccgtggtga agaaatagag ccgctggata
    32401 acgaattgct ggcagagctg gcgcaatcca gccactggca agccgttgtg caattgcatc
    32461 aggtgttgtt gaaggaattc gcactggaag ttaacccgtg tgaagaaggc tatttgctgg
    32521 ccaaccttta tggattatgg atggctgcta acgaagaggt ttgaagctcc cgacaaatcc
    32581 acgtcgcaca acctaaatat tcaaaaaaat gaatatttaa ttcaaggaac agaaacgagg
    32641 caacaagatg tttgtagaag cattgaaacg ccagaacccg gcgctgattt ccgccgcact
    32701 aagtttgtgg cagcagggca agatcgcccc ggacagctgg gtgatcgacg tggatcaggt
    32761 actggaaaac ggtaagcgac tgattgagac ggcgcggctt tacggcattg aactgtatct
    32821 gatgaccaag cagtttggcc gtaacccatg gctggcggaa aaattgctgg cattaggtta
    32881 cagcggcatt gtggcggtgg attacaaaga ggcgcgagtc atgcgccgcg ctggtttgtc
    32941 ggtggcacat caggggcatc tggtacaaat cccttgtcat caggttgctg acgccgttga
    33001 acagggcacc gacgtcatca ccgtgtttac tctcgacaaa gcgcgggaag tttctgcggc
    33061 agcggtgaag gccggacgtg tgcagtttgt gctgctcaaa gtttatagcg acgatgattt
    33121 tctttatccg gggcaggaga gcggttttgt acagcattcg ctgcacgagg tggttgctga
    33181 aattaagaaa ctgccggggc tgcatttagc cggacttacc catttccctt gcctgctttg
    33241 ggatgaggct gccggaaaag ttttgccgac accgaatctt cacacgctga tacaggcacg
    33301 ggatcaactg gcgaaatctg gtattgcact tgagcaactg aacgcgcctt cagcgaccag
    33361 ctgcacttcg ctgccattac tggcgtaata cggtgtgact catgccgaac ccggtcatgc
    33421 gctgacgggc actattccgg caaaccagca gggcgatcag tctgaacgta tcgcgatgct
    33481 ctggttgagt gaaatctccc atcatttccg tggcgacagc tactgctacg gcggcggtta
    33541 ctaccgtcgt ggtcatgcgc aacatgcgtt ggtgtttacg ccagaaaatc aaaagattac
    33601 tgaaaccaat ctcaaaactg tggatgacag cagtatcgac tacaccctgt cgctggcagg
    33661 cgagtttccg gtaagcagtg cagtggtgct ctgtttccgc acgcagattt ttgtcacccg
    33721 tagcgatgtg gtgctggtgt ccggtattca ccgtggcgaa ccgaaaatcg ttggtcgtta
    33781 tgacagtctt ggaaactctc tgggggcgta atggcgcgat ttgtggtgtt agtgattgat
    33841 agctttggcg taggggcaat gaaagatgtc acgctggtgc gtccgcaaga tgcgggagcg
    33901 aatacatgtg gtcacatcct gagccagttg ccgcatttgc agctaccaac gctggagaag
    33961 ctggggctaa tcaacgcatt gggttatgcg ccaggcgata tgcagccgtc agattccgca
    34021 acctggggcg tggcagagct gcaacatgaa ggtggcgata cctttatggg gcatcaggaa
    34081 attttaggca cgcgcccgtt accgccgctg cggatgcctt ttcgcgatgt gattgaccgt
    34141 gttgagcagg cattagtatc cgccggttgg caggtggagc gccgtggcga tgaactgtaa
    34201 tttctgtggg tcaatcaggc ggttgcgatt ggcgataatc tcgaggcgga tttaggccaa
    34261 gtctataaca ttaccgccaa tctctctgtg atctcttttg acgacgcaat caaaattggt
    34321 cgtatcgtgc gtgagcaggt acaggtcggt cgggtcatta catttggtgg cctgttaacc
    34381 gacagtcaac gcattctcga tgccgcagaa agcaaagaag ggcgctttat tggtatcaat
    34441 gcgccgcgtt ctggcgctta tgacaacggc ttccaggtcg tgcatatggg ctacggtgtc
    34501 gataaaaaag tgcaggtgcc gcaaaaactg tatgaggcgg gcgtgccaac cgtgctggtg
    34561 ggtaaggtgg cagatatcgt cagcaatcct tatggcgtga gctggcaaaa cctggtggat
    34621 agccagcgga ttatggatat caccctcgac gaatttaaca cccatccgac ggcgtttatt
    34681 tgcaccaata tccaggaaac cgacctcgct ggtcatgcag aagacgtcgc acgttatgcc
    34741 gaacgtttgc aggtcgttga ccgtaacctt gcccggcttg ttgatgcgat gcagccagat
    34801 gattgcctgg tcgtgatggc ggatcacggc aacgatccga ccattggtca cagccaccat
    34861 acccgcgaag tggtgccgct gctggtttat cagcaaggcc tggctgctac gcagctcggt
    34921 gtgcgcacca cgctttctga tgtgggagct accgtgtgtg aatttttccg tgcgccaccg
    34981 ccacaaaatg gtcgctcttt tctttcctcc ctccggtttg caggagacac cctatgagtt
    35041 ttgatccgac gggttacacc ctggcccatg agcatctgca tattgatctc tccggcttta
    35101 aaaacaacgt ggactgccgc cttgatcagt atgcgttcat ttgccaggag atgaacgacc
    35161 tgatgacccg gggtgtacgt aatgtgattg agatgaccaa ccgttacatg gggcgcaatg
    35221 cgcaatttat gctcggtgta atgcgcgaaa ccgggatcaa cgtggtggcc tgtactggtt
    35281 attaccagga tgcgtttttc ccggaacatg tggtgacccg cagcgtgcag gaactggcgc
    35341 aggagatggt cgatgaaatt gaacagggta tcgatggcac ggagctgaaa gccgggatca
    35401 tcgcggagat cggctccagc gaaggaaaga ttacgccgct ggaagagaag gtatttattg
    35461 ccgctgcgct ggcgcataac cagaccggac gcccgatctc cacgcatacg tcgttcagca
    35521 ccatggggct ggaacaactg gctctgttgc aagcccacgg agttgatctt tcgcgcgtca
    35581 ccgttggtca ctgcgatctg aaagacaacc tcgacaacat tttgaagatg atcgatctcg
    35641 gcgcgtacgt gcagttcgac accattggta agaacagtta ctacccggac gaaaagcgca
    35701 ttgcgatgct ccacgcgcta cgtgaccgtg ggttgctgaa ccgcgtcatg ctgtcgatgg
    35761 atattacgcg ccgctcccat ttaaaagcca atggtggtta tggctatgac tatttattga
    35821 ccacctttat tccgcaattg cgccagtcag gattcagtca ggccgatgtg gatgtgatgt
    35881 tacgtgaaac tccctctcaa tttttccaat aaggacagac tcatgaaaaa gattggcgtt
    35941 gcaggcttac agcgtgagca gattaaaaaa accattgaag cgacggctac tggcagtttt
    36001 gaagttttca ttcacaacga catggaagcg gcaatgaaag tgaaatccgg gcaactggat
    36061 tactacatcg gtgcgtgtaa taccggtgcg ggcgcggcat tgtcgattgc catcgcggtg
    36121 attggctata acaaaagttg caccattgcc aaaccaggca ttaaagcgaa agacgagcat
    36181 atcgccaaaa tgatcgctga aggaaaagtg gcgtttggcc tttccgttga gcacgtcgaa
    36241 cacgcgattc cgatgctgat taaccatctg aaataaaagg cacgactatg gatctgtata
    36301 ttcagattat cgtggtggcg tgcctgacgg gtatgacatc gcttctggcg catcgctcgg
    36361 cggctgtttt tcatgacggt atccgcccga tcctgccgca actgattgaa ggctatatga
    36421 accgtcgcga ggcggggagt atcgcttttg gtctgagcat tggttttgtg gcctcggtgg
    36481 ggatctcttt taccctgaaa accgggctgc tcaacgcatg gttactcttt cttcctaccg
    36541 atatcctcgg cgtactggcg ataaacagcc tgatggcgtt tggtcttggc gctatctggg
    36601 gcgtgttgat ccttacttgc ctgttgccag taaaccagct gctgaccgcg ctgccggtgg
    36661 atgtattagg tagcctcggg gaattaagct cgccggtggt ttctgctttt gcactgttcc
    36721 cgctggtggc gattttctac cagtttggct ggaagcaaag tctggtcgcc gccgtggttg
    36781 ttctgatgac ccgtgtggta gtcatgcgct atttcccaca tcttaaccct gaatccatcg
    36841 aaatctttat tggcatggtg atgctgctgg ggatcgcgat aactcacgac ctgcgtcatc
    36901 gtgatgaaaa tgatattgat gccagcgggc tttcggtgtt tgaagaacgc acgtcgcgga
    36961 ttatcaaaaa cttaccctat atcgccatcg tgggagcatt gattgccgcc gttgccagca
    37021 tgaagatttt cgccggcagt gaagtgtcga tcttcactct ggagaaagcg tactccgcag
    37081 gcgtaacgcc ggaacaatcg caaacgctga tcaaccaagc ggcactggca gaatttatgc
    37141 gcgggctggg ttttgtaccg ttgattgcca ccaccgcctt agcaacgggt gtgtatgcag
    37201 ttgcgggctt tacctttgtt tatgcggtgg gctatctctc gccgaatccg atagttgcag
    37261 cggtattagg cgcagtggtt atttcggcgg aagtcttgct gcttcgttcg atcggcaaat
    37321 ggctgggacg ctacccgtcg gtgcgtaatg cgtcggataa catccgtaac gccatgaata
    37381 tgctgatgga agtggcgctg ctggtcggtt cgattttcgc agcaattaag atggcgggtt
    37441 ataccggatt ctctatcgcg gttgccattt acttcctcaa cgaatccctg ggccgtccgg
    37501 tacagaaaat ggcggcaccg gtcgtggcag taatgatcac cggtattctg ctgaatgttc
    37561 tttactggct tggcctgttc gttccggctt aaggaaggca cctatgaaga cgtttcctct
    37621 gcaaagtctg acgattattg aggcgcagca aaagcagttt gcgctggtgg atagcatttg
    37681 tcgccatttc cccggcagcg agtttcttac tggcggtgat ttaggcttaa cgccaggact
    37741 gaatcaaccg cgcgttaccc agcgtgtgga acaggtgctg gctgatgcat ttcacgcaca
    37801 ggctgcggcg ctggtgcagg gtgcgggtac tggcgcgatt cgtgcggcgt tggcggcttt
    37861 gctcaaaccg gggcagcgtc ttctggtaca tgatgcgcct gtttacccga cgacacaggt
    37921 cattattgag cagatggggc tgacgcttat tactgctgat ttcaatgacc tgtcggcact
    37981 gaagcaggtc gtcgacgagc aactaccgga tgcggcgctg gtgcagcata cgcgccagca
    38041 gccgcaggac ggctacattc tggcagatgt gctggcaaca ttgcgctcgg ctggcgttcc
    38101 tgcattaacc gatgacaact atgcggtgat gaaagtggcg cgaattggct gcgaatgcgg
    38161 ggcaaatgtc tcgacttttt cctgcttcaa gctgtttggg ccggagggtg ttggcgcggt
    38221 ggtaggcgat gccgatgtta ttagtcggat tcgctccacg ctctattccg gcggcagcca
    38281 gattcagggc gctcaggcgc tggaagtctt gtgtgggctg gtgtttgcgc cagtgatgca
    38341 cgcggtgcag gcgggggtat ctgaacggtt gctggctttg cttaacggtg gtgcggtgcc
    38401 ggaagtgaaa agcgcggtga ttgctaatgc gcagtcgaag gtgttgattg tcgagtttca
    38461 tcagccgatt gccgccagag tgctggaaga ggcgcaaaag cgcggtgcct tgccttaccc
    38521 ggtgggtgca gagtcgaaat atgaaatccc gccgctcttt tatcgccttt ccggaacgtt
    38581 tcgccaggcg aatccacaat cagaacattg tgcgattcgc attaacccga atcgcaacgg
    38641 tgaagagacg gtgctgcgga ttttgcgtga gagtattgcc agtatttaat gctggatagc
    38701 accgtcaagc taaattccgt actgaacggt cccctcgccc ctttggggag agggttaggg
    38761 tgaggggaac gtacgggatg taaaattcaa cgcgccagca tcgccggatg cgatgctggc
    38821 gcatcttatc cggcctacgg ttaatgtctt ttattatcaa tcgttaaggt gatgcgattg
    38881 gcgcggcaga agagttcgga gacgtgcacc ggtttgtcat tctgatcaaa ggcgattttg
    38941 ctgatgcgaa acaacggttc gcccagttcg catttcagcc attttgcctg cgggcgggtg
    39001 gcggcaaaga tatcgatggt ctttttgtcg ctgaccactc gcgtgtcaaa acgctcctga
    39061 aataactgat aggtggagct tccttcgacg taaatctcat caaagtcagg ataacgcgac
    39121 agcgggatcc atgaactatc aataaacagc ggctctttat cgagatacat cacccggcag
    39181 agatggaaca cttcgctgtt tccggggatg ttcagctttt cgcaaaacgg cgcggcgctg
    39241 acccgttcct gttcgatcac tttctctttc gtcgccttgc cttgtgagac gccaaaatcg
    39301 gtaaaaccac tgacagtaag cagggcgttt tcaactttct ggctttgtac aaaggtgcct
    39361 ttgccctgcc agcggatcag tacgccgtct gccactaagt cgctgatagc tttgcgaata
    39421 gtaatgcggc tgacgttata ttgcgtacaa agctcgtttt cggtagggat ctgttgcccg
    39481 gcctggtaaa ccccctgcgc gatatcatcc agcagccgct ggcggacggt agcgtagagg
    39541 agttgatgag agtagcggtc cgtagctgac ataatcgacc ctgaaataaa gaatgaatta
    39601 cacggattat agggtcgttt tccggcaagt aacagcacgt tggcgcggag atgctcacgc
    39661 caacgttata cctaccaggc accgtgatac tgaatggttt tcgccgcgca cgccgttccc
    39721 tgcgccatcg cctgcggtaa tgtcatcccc gcagaccagc cgcaaaggaa tccggcaatg
    39781 aacgaatctc cggcacccat ggtgtcgata accgtcaccg gttcaggagc ctgacgccag
    39841 aactgcgcgc catcccaggc aatgctgccg ttttcaccca gcgtgacaat cactgttcct
    39901 gcgccacggg caacaatcgc tttcatcttc agacgcagcg cttcgtcttc ttgcggtgcg
    39961 gaggcaaagg caaaatcgag atgcggcacc agtgtctgcc agagcgggct gtcccacttg
    40021 tcggagaagt cgaaagcggt aagtttgccc gcagcgtgca gctgtgggaa tgcgtcttcc
    40081 gcatgtcccc agattgccgc gtgcacaatg tcatactgcg ccagccaggc gtaatcctct
    40141 tcactcaggg caaagtcggc catcacgcct tcggtgtagt cgccaaaaac gcgatcattg
    40201 tcgtgcagtt ccacctgagt ttgtgcggta acgccgtgtt tcgtatggac atggctgata
    40261 tcgacgccca tgcgggcgag atcctgcttc agctttgtgc cgtagtcatc gtcacccacc
    40321 aaggtaatgc atcccggctg tatgccgtag cgagtgcagt acaccgccac attgaccgca
    40381 ttaccgccag aaaacgcttt attcagttgc gggtagatat cgacgcagtt atcgccgatt
    40441 gtcgccaggg ttttcatctc tcatcctccg gcagcagcgc gcgaaagcgt tccagcgcct
    40501 ggcgggcata gagtctgggc tcgttcatat acatcgtcac cagctccacc gtacagtaac
    40561 cctcatagcc ccgctcaata atatcgcgca tcagttcccg cagcggcatt ttgccttctc
    40621 caggaatgta atgcgtgtcg ctggccccgt cgctgtcgac aatatgcaga tgacgataat
    40681 ttatcgccca gtttgtcgaa ataactcatc accggttccg cctggacata cggcgcgcaa
    40741 atgtcgacca tgctgaacag gcgcggcgaa ggcaccagcg ccagcgcatg aagcacatca
    40801 ttagcattac acacgacgtt cgattcatac ggcgttaacg gttcgaggat tagatccatg
    40861 ccgatgtttt ccgcgtactc acacagttcg cttaggttct ctgccagccg tccccagata
    40921 acattaggtg gcgtgagata gcctgcgtgg gccgcggaaa tcagcgtata acccgcgttc
    40981 atctcttttg ccatatccat cgccagcttg atcatgtcga ggctttcgcg acgcatatgt
    41041 tcatcgccca gcatcatgtt atacggatag ccgttggttt ctggcgtata gccgataatc
    41101 ggcatctgat acgtctgcgc cagcgccttg atttgtttga tgccgcccgc ttttaagtcc
    41161 ggcgcgaacg cgtgcgggcg accgccccaa atttcgatgc cgtcgtaacc cagctcgctt
    41221 gcatcacgaa atgcgtgttc aataggcagc cgctggtggc cgcaggtaaa catacctgtt
    41281 ttcatcgggt aactccttgc gtaaatccgg cccgttgccg ggccgggaga gggattaata
    41341 ttccaccaga ccaccgtaat agcggcgttc atccgggttg tgatctttgt aaatagacag
    41401 gtagtagcag agccactcca ttggcacgaa catcaggaac ggtgccagcc acgggtgcag
    41461 cccttgcgaa atttcggcgt aatcgatgac gatcacgttg tcagtacgct gtttaacaaa
    41521 gttaatggcg cgttcggtgg tgtggcgact ttcatcattg ccgagcagga acaggaacgg
    41581 aacgcccggt tcgacaatct ccagcgggcc atggcggaac tctccgctct caatcacgca
    41641 gccgtgcgtc caggtaaatt ccatcagcgt tacaatgcct tctttgtaac ccagcggacg
    41701 cagcggaccc gcagcaacgg tataaatcat cggccactgg ctggccagtt caccaagctg
    41761 gcggcctttt tcttcccagg tgcgtaccag atgaccgagc gcattcggca acggcttgag
    41821 atcgtttttg atcttgccga tttccgcgtt cggcgcgagg cgggtgatca tctccagcac
    41881 cacgctgtag cagagcagca ggtgaatttc ccagatacag tcggcctgat aatcaatgct
    41941 aaattccgcc gccgaggtaa tcgggctgtc cgcgcgtttg gtgaacgccg cagtgagtgc
    42001 gccgcaggcc cggcccagct ccagcgcttt gattacctct tccgttttac cgtagtcaga
    42061 aacgccaatc accgcgcaac gatcgtcgag gcgatacggg gtgttatcgc agaactccca
    42121 gccggaaatg gcgtagacct gaagatcgga aaagcgatcc gccagatgtt tcgccgtttg
    42181 cgccgcgttg agtggcgagc cgcaggcaac gaaataaata cgatcaatgt cgcgcttcac
    42241 catctcttcc acgatggcgt gcaccagcgg aacgtcatgg ctgagaactt tttccacttc
    42301 ctgaaccata ttttcggtga ccagaaagtc cacggtgctt ttatcaatat ccaacatttt
    42361 attttctcca gataacatta cgacaaagcg ttgagctggc ggctacgctt cgcccagaag
    42421 gcgtaagcag gcagaccagt agcaataacg atgacggcgc agataaggcc gggaatcggt
    42481 gcccagacaa aggttgaggc gaccagaatg aggcttgacg caatggcgag ggtggtcatc
    42541 agcccgaaag ccggagtacg ccacagcggt ttgtaatcgt cgcgtttacg acaccagatg
    42601 atggagccga aggtgagggt atttttgaaa cacatcacca gggtgaaata acccagcagg
    42661 ctggtgagat cggaaacgaa gatgaagaag atccccagcg ccccttgcag gatgatggag
    42721 acatccggcg tgttgtattt cggatgcaca tggccgaagc atttaaagaa caggttgtct
    42781 ttcgccatcg cgtattccag ccgcggctgg tacatcacgc agctggaaag cgaaccaaga
    42841 atgacgatca tcgccgtgat ggcaacaaag atcccagcgg tgctgccgag tgcggggatc
    42901 caggtcaggg cgtcggaaat cggcgtttca gaattggcga gtttgtcgaa gggcatcagg
    42961 ccggaaatca ccagcgccag cagggtgtag agcaccagaa ccagcagaca ggaaccaatc
    43021 agcgctcgtg gcatggtttt tccggggttt ttaatttcgc cggtcatata acagatagag
    43081 gccatgccgg tatacgacca actggtggca gagatccccg ccagcagcgc cataaagctg
    43141 cccgttgcgc caatcgcagt ggtggtaggg gcggcaaaat tctccgcttt aaaccagaag
    43201 atcccaaggc caatgacgat agtgaacggg ataattttgg cgatggtaat tagcgtctga
    43261 aacgctgcgc cgccttcaac ggagcgcagg tgtagcaaca taaaggcgat aattaatccg
    43321 gcggcgataa atttaccgag caacggatcg ataggcgtta aaaagccaag attgctgaca
    43381 atcgccagcg ccataatcga caatgacggc gcatcgttgg cccagaagct ggcccagccg
    43441 gagaggaaag ccagcggtcg gcttccggca tttttcagat aaacataatc tgcgccattt
    43501 tccggataag cggtggatag ttccgcatag acgcacattt gcgggatcac aattaacccg
    43561 ccaatgacaa acgcgagcac cgtaagccac ggcgtgcccg ctgcttttgc cacttcaccc
    43621 acagatacaa aaataccgga gccgacggtt gtcccgacgg cgattgcaag aacggcccaa
    43681 aatccgagct tgcgttggag ttcctggctt ccc
//
LOCUS       DADMKF010000013        43570 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.013, whole genome shotgun sequence.
ACCESSION   DADMKF010000013 DADMKF010000000
VERSION     DADMKF010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 43570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43570
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.013"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            <1..664
                     /locus_tag="JNC24_000681"
     CDS             <1..664
                     /locus_tag="JNC24_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HAY9157511.1"
                     /translation="SQAITNGFQRSMFSNEAGMGSTPNAAAAAASWPPHPAAQGIVQM
                     IGIFIDTLVICTASAMLILLAGNDTTYMPLEGIQLIQKAMRVLMGSWGAEFVTLVVIL
                     FAFSSIVANYIYAENNLFFLRLNNPKAIWCLRICTFATVIGGTLLSLPLMWQLADIIM
                     ACMAITNLTAILLLSPVVHTIASDYLRQRKLGVRPVFDPLRYPDIGRQLSPDAWDDVS
                     QE"
     gene            734..1510
                     /gene="yaaA"
                     /locus_tag="JNC24_000682"
     CDS             734..1510
                     /gene="yaaA"
                     /locus_tag="JNC24_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HAY9157512.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQHLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPENARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEASSSNGELVFKRYEQR"
     gene            complement(1663..1959)
                     /locus_tag="JNC24_000683"
     CDS             complement(1663..1959)
                     /locus_tag="JNC24_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HAY9157513.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAPHDHHGGHGPGKHHR"
     gene            complement(2173..3459)
                     /gene="thrC"
                     /locus_tag="JNC24_000684"
     CDS             complement(2173..3459)
                     /gene="thrC"
                     /locus_tag="JNC24_000684"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HAY9157514.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIAGDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSLGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALCDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFAALRKLMMNHQ"
     gene            complement(3460..4392)
                     /gene="thrB"
                     /locus_tag="JNC24_000685"
     CDS             complement(3460..4392)
                     /gene="thrB"
                     /locus_tag="JNC24_000685"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HAY9157515.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVTPVDGALLGDVVTVEAAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCLELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(4394..6856)
                     /gene="thrA"
                     /locus_tag="JNC24_000686"
     CDS             complement(4394..6856)
                     /gene="thrA"
                     /locus_tag="JNC24_000686"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HAY9157516.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEGELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     MERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTSVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHQLRYAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELVKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     gene            complement(6938..7003)
                     /gene="thrL"
                     /locus_tag="JNC24_000687"
     CDS             complement(6938..7003)
                     /gene="thrL"
                     /locus_tag="JNC24_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HAY9157517.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(7217..7903)
                     /locus_tag="JNC24_000688"
     CDS             complement(7217..7903)
                     /locus_tag="JNC24_000688"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HAY9157518.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDVIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADQHQLQALRERVMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(8303..8443)
                     /locus_tag="JNC24_000689"
     CDS             complement(8303..8443)
                     /locus_tag="JNC24_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9157519.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            8539..9255
                     /gene="arcA"
                     /locus_tag="JNC24_000690"
     CDS             8539..9255
                     /gene="arcA"
                     /locus_tag="JNC24_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HAY9157520.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(9315..10667)
                     /gene="creD"
                     /locus_tag="JNC24_000691"
     CDS             complement(9315..10667)
                     /gene="creD"
                     /locus_tag="JNC24_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HAY9157521.1"
                     /translation="MLKSSLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELDAPNITFGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLKLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREVSESGFQAQWQSSWFANNLGERFASGNDTGWENFL
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHIGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSVDSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTTDDELRIWK
                     "
     gene            complement(10725..12149)
                     /gene="creC"
                     /locus_tag="JNC24_000692"
     CDS             complement(10725..12149)
                     /gene="creC"
                     /locus_tag="JNC24_000692"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HAY9157522.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAQG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMDGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPDLGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVTPTEVNVAAEPALLDQALGNLLDNAIDFTPESGRITLS
                     AEVDQEHVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(12149..12838)
                     /gene="creB"
                     /locus_tag="JNC24_000693"
     CDS             complement(12149..12838)
                     /gene="creB"
                     /locus_tag="JNC24_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HAY9157523.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSSPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDTHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(12851..13324)
                     /gene="creA"
                     /locus_tag="JNC24_000694"
     CDS             complement(12851..13324)
                     /gene="creA"
                     /locus_tag="JNC24_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HAY9157524.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            13535..14404
                     /gene="robA"
                     /locus_tag="JNC24_000695"
     CDS             13535..14404
                     /gene="robA"
                     /locus_tag="JNC24_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HAY9157525.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(14401..15048)
                     /gene="gpmB"
                     /locus_tag="JNC24_000696"
     CDS             complement(14401..15048)
                     /gene="gpmB"
                     /locus_tag="JNC24_000696"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HAY9157526.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            15100..15621
                     /gene="yjjX"
                     /locus_tag="JNC24_000697"
     CDS             15100..15621
                     /gene="yjjX"
                     /locus_tag="JNC24_000697"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP phosphatase"
                     /protein_id="HAY9157527.1"
                     /translation="MHQVICATTNPAKIQAILQAFHEIFGEGSCHIASVAVESGVPEQ
                     PFGSEETRAGARNRVANARRLLPEADFWVAIEAGIDGDSTFSWVVIENTSQRGEARSA
                     TLPLPAVILQKVREGEALGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRASVYHQAVIL
                     ALSPFHNAVYQAL"
     gene            complement(15706..16032)
                     /gene="trpR"
                     /locus_tag="JNC24_000698"
     CDS             complement(15706..16032)
                     /gene="trpR"
                     /locus_tag="JNC24_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HAY9157528.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(16122..18059)
                     /gene="sltY"
                     /locus_tag="JNC24_000699"
     CDS             complement(16122..18059)
                     /gene="sltY"
                     /locus_tag="JNC24_000699"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HAY9157529.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQVQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDVIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQVDLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSALTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            18270..19937
                     /gene="ettA"
                     /locus_tag="JNC24_000700"
     CDS             18270..19937
                     /gene="ettA"
                     /locus_tag="JNC24_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HAY9157530.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(20244..21476)
                     /locus_tag="JNC24_000701"
     CDS             complement(20244..21476)
                     /locus_tag="JNC24_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide
                     kinase/transcriptional regulator NadR"
                     /protein_id="HAY9157531.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIDTVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQR"
     gene            complement(21497..22879)
                     /gene="radA"
                     /locus_tag="JNC24_000702"
     CDS             complement(21497..22879)
                     /gene="radA"
                     /locus_tag="JNC24_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HAY9157532.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSMMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(22928..23896)
                     /gene="serB"
                     /locus_tag="JNC24_000703"
     CDS             complement(22928..23896)
                     /gene="serB"
                     /locus_tag="JNC24_000703"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HAY9157533.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            24002..24646
                     /locus_tag="JNC24_000704"
     CDS             24002..24646
                     /locus_tag="JNC24_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HAY9157534.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            24674..25690
                     /gene="lplA"
                     /locus_tag="JNC24_000705"
     CDS             24674..25690
                     /gene="lplA"
                     /locus_tag="JNC24_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
                     /protein_id="HAY9157535.1"
                     /translation="MSTLRLLISDSYDPWFNLAVEECIFRQMPATQRVLFLWRNADTV
                     VIGRAQNPWKECNTRRMEEDNVRLARRSSGGGAVFHDLGNTCFTFMAGKPEYDKTIST
                     SIVLNALNALGVSAEASGRNDLVVKTVEGDRKVSGSAYRETKDRGFHHGTLLLNADLS
                     RLANYLNPDKKKLAAKGITSVRSRVTNLTELLPGITHEQVCEAITKAFFAHYGERVEA
                     EIISPDKTPDLPNFAETFARQSSWEWNFGQAPAFSHLLDERFSWGGVELHFDVEKGHI
                     TRAQVFTDSLNPAPLEALAGRLQGCLYRADMLQQECEALLVDFPDQEKELRELSTWIA
                     GAVR"
     gene            complement(26146..26865)
                     /gene="deoD"
                     /locus_tag="JNC24_000706"
     CDS             complement(26146..26865)
                     /gene="deoD"
                     /locus_tag="JNC24_000706"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAY9157536.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGIDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(26945..28168)
                     /gene="deoB"
                     /locus_tag="JNC24_000707"
     CDS             complement(26945..28168)
                     /gene="deoB"
                     /locus_tag="JNC24_000707"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HAY9157537.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAVGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            complement(28220..29542)
                     /gene="deoA"
                     /locus_tag="JNC24_000708"
     CDS             complement(28220..29542)
                     /gene="deoA"
                     /locus_tag="JNC24_000708"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HAY9157538.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVIHAKDENSWQEAAKAVKAAIKLADKAPESTPTVYRRISE"
     gene            complement(29669..30448)
                     /gene="deoC"
                     /locus_tag="JNC24_000709"
     CDS             complement(29669..30448)
                     /gene="deoC"
                     /locus_tag="JNC24_000709"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HAY9157539.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGNEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            30706..32256
                     /locus_tag="JNC24_000710"
     CDS             30706..32256
                     /locus_tag="JNC24_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HAY9157540.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQDEIDVRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAELKQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIAN
                     GPVHDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYC
                     QQQIAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCKKEG
                     IAARYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGSSSDIGTTPGAR
                     LPYGDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMRE
                     FTANVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISH
                     EQQMRFSQ"
     gene            32228..33091
                     /gene="yjjW"
                     /locus_tag="JNC24_000711"
     CDS             32228..33091
                     /gene="yjjW"
                     /locus_tag="JNC24_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HAY9157541.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKRCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQHLTGRDNQQIKRSICLLAERGKLAELRLLVIPDQ
                     VDYLHHIDELATFIKRLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
     gene            complement(33607..34389)
                     /locus_tag="JNC24_000712"
     CDS             complement(33607..34389)
                     /locus_tag="JNC24_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAY9157542.1"
                     /translation="MICRFIDTHCHFDFPPFSGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAEKYQPLYAALGLHPGMLEKHSDVSLDQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRIHDKLAMHLKRHDLPCTGVVHGFS
                     GSLQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAVRVFDVLCELRPEPEDEIAEVLLNNTYALFSVSG"
     gene            complement(34386..35459)
                     /locus_tag="JNC24_000713"
     CDS             complement(34386..35459)
                     /locus_tag="JNC24_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HAY9157543.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRIFEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            complement(35581..35742)
                     /locus_tag="JNC24_000714"
     CDS             complement(35581..35742)
                     /locus_tag="JNC24_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HAY9157544.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            complement(35869..36474)
                     /gene="osmY"
                     /locus_tag="JNC24_000715"
     CDS             complement(35869..36474)
                     /gene="osmY"
                     /locus_tag="JNC24_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HAY9157545.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRHVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            complement(36867..38456)
                     /gene="prfC"
                     /locus_tag="JNC24_000716"
     CDS             complement(36867..38456)
                     /gene="prfC"
                     /locus_tag="JNC24_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HAY9157546.1"
                     /translation="MTLSPYLQEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     SRLKSEYSVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            complement(38547..39224)
                     /gene="yjjG"
                     /locus_tag="JNC24_000717"
     CDS             complement(38547..39224)
                     /gene="yjjG"
                     /locus_tag="JNC24_000717"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HAY9157547.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHNREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            complement(39239..39685)
                     /gene="rimI"
                     /locus_tag="JNC24_000718"
     CDS             complement(39239..39685)
                     /gene="rimI"
                     /locus_tag="JNC24_000718"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAY9157548.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRVLLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            complement(39654..40067)
                     /gene="holD"
                     /locus_tag="JNC24_000719"
     CDS             complement(39654..40067)
                     /gene="holD"
                     /locus_tag="JNC24_000719"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HAY9157549.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIAIPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            40170..41201
                     /gene="rsmC"
                     /locus_tag="JNC24_000720"
     CDS             40170..41201
                     /gene="rsmC"
                     /locus_tag="JNC24_000720"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HAY9157550.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSRQMGDNARFSLVATVDDVADCDMLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANCVEGEVFASNVFSEVKGRFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            41469..41555
                     /locus_tag="JNC24_000721"
     tRNA            41469..41555
                     /locus_tag="JNC24_000721"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:41503..41505,aa:Leu,seq:cag)
     gene            complement(41594..41830)
                     /locus_tag="JNC24_000722"
     CDS             complement(41594..41830)
                     /locus_tag="JNC24_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HAY9157551.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYVLLPSCLALIAAIMLMIMNLNQG"
     gene            41971..42759
                     /gene="fhuF"
                     /locus_tag="JNC24_000723"
     CDS             41971..42759
                     /gene="fhuF"
                     /locus_tag="JNC24_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HAY9157552.1"
                     /translation="MAYRSAPLYEDIIWRTHLQPQDAGLAQAVRATIAKHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQLYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRVACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGDCTLK"
     gene            complement(42797..43474)
                     /gene="bglJ"
                     /locus_tag="JNC24_000724"
     CDS             complement(42797..43474)
                     /gene="bglJ"
                     /locus_tag="JNC24_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HAY9157553.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIADDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCEA
                     SNVLHPY"
     gene            complement(43432..>43570)
                     /locus_tag="JNC24_000725"
     CDS             complement(43432..>43570)
                     /locus_tag="JNC24_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HAY9157554.1"
                     /translation="AIHSNAEMYAWINSAQGARELNLPSVYGDAAEWNTAELRREMSH
                     S"
BASE COUNT        10351 a        11915 c        10975 g        10329 t
ORIGIN      
        1 aagccaggcg attactaacg gttttcagcg cagtatgttt tccaatgagg cgggaatggg
       61 ttcgacgcca aacgcggcag cggcagcggc gtcctggcct ccgcatccgg cagcgcaagg
      121 gattgtccag atgattggca tttttatcga caccctggtc atctgtacgg caagcgccat
      181 gctgatatta ctggcgggta acgacacaac ctacatgccg ctggaaggta ttcagcttat
      241 ccagaaggcg atgcgggtgc taatgggttc ctggggtgct gagtttgtta ccctcgtggt
      301 tattctgttt gccttcagct ccatcgttgc caactacatt tatgccgaaa acaatctctt
      361 ctttttacgc ctgaacaacc ctaaagcgat ctggtgtttg cggatctgca ccttcgcaac
      421 ggtcatcggc ggcaccttgc taagtcttcc gctgatgtgg caactggcag atatcataat
      481 ggcctgcatg gctattacca atttgaccgc cattttactg ctctcgcctg tggttcatac
      541 cattgccagt gattatctac gccagcgtaa actcggcgtg cgcccggtgt ttgatccgtt
      601 gcgttatccg gatatcggtc gccagctttc tccggacgcg tgggatgatg tttcgcagga
      661 gtaatcacaa ctatcgatca actcattctc tttttttgct aaagtcggca taaatttcct
      721 gcaaggactg gatatgctga ttcttatttc acctgcgaaa acgcttgatt accaaagccc
      781 gttgaccacc acgcgctata cgctgccgga gctgttagac aattcccagc acttgatcca
      841 tgaagcgcgg aaactgacgc ctccgcagat tagcacgctg atgcgcatca gcgacaaact
      901 ggcgggtatc aacgccgctc gctttcatga ctggcagcca gatttcacgc cggagaatgc
      961 ccgccaggct attttggcgt ttaaaggtga tgtctacacc ggcttgcagg ccgaaacttt
     1021 cagcgaagac gatttcgatt ttgcccaaca gcatttgcga atgctttccg gcttgtatgg
     1081 cgtactgcgc ccgctcgatt taatgcagcc ttatcgtctg gaaatgggga tccgtcttga
     1141 gaatgcccga gggaaagatc tgtatcaatt ctggggagat atcatcacca acaagctgaa
     1201 cgaggcgctc gcagcacaag gcgataatgt ggtgattaat ctggcgtcag atgagtattt
     1261 taaatcggtg aagccgaaga aattgaatgc cgagattatc aagccggtgt tcctcgatga
     1321 gaagaacggc aaatttaaga taatcagctt ctacgctaag aaagcacgcg gtctgatgag
     1381 tcgtttcatt attgaaaatc ggctgaccaa accagagcaa ctgactggtt ttaatagcga
     1441 aggttacttc tttgatgaag cttcctccag caatggcgaa ctggtgttta aacgctacga
     1501 gcagcgttaa gtttgcagtc aatgccggat gcggcgtgaa cgccttatcc ggcctacaaa
     1561 agcatgcaga ttcaatatat tgcagaattc acgtaggcct gataagacgc gccagcgtcg
     1621 catcaggcgc tgaatgccgg attgttaccc ggcatttgtc atttagcggt gatgtttgcc
     1681 tggaccatga ccgccgtgat gatcatgagg agctttctta tggtggcgcg gcggtggcgg
     1741 cggtccgtgt gggtgccagc gattgcctcg ccattcataa tgttgtttcc accagccgtg
     1801 gtcgcgccag tgacctccat cccagtaata gccacgatta tcacgatcgc ctatctgtaa
     1861 ttttactgac gggactaacg taatttccgc agcctgtgct gccatgggag cgaccagaac
     1921 cagggaaagt gcgagtacga tagattgcat ctttttcaca ggttactcct tttatcattg
     1981 cccgttatgg gtcgatatcc ggagattacg cgatgagaac gacggttgtt attctccgca
     2041 atcctaatct ctaagttacc gcatttattg agaatttctc ctttttccct gtcgtttttc
     2101 tccataattt cttcataaaa aagccgggct gcataaaagc aaacccggcc tgattgagat
     2161 aatgaataga ttttactgat gattcatcat caatttacgc aacgcagcaa aatcggcggg
     2221 cagattatgt gaaagcaagg gtaaatcagc acgttctgcc agctcttttg gcagatccaa
     2281 cgtttcaccg agaatcgctt ccacgctctc tttaaatttc gccggatgcg cggtgccgag
     2341 gaacaagcca tattcgcctg gattcaactg gtcacacagc gcacgatacg ctacggcagc
     2401 gtgcggctcc gaggtgtagc ccagttcttt taactcacgc attgtctgtt gcgtggtttc
     2461 atcatccacg gctgcataac ccagctcttt cagttgccag attttgcggc ggaacaactc
     2521 ttccacacgc ggccagttgt tcggctgact cacgtccatc gcgttggata acgtcgcctg
     2581 agtcgctttg ggtgaccact gaccgtcgtg caggaaacgt ggcacggtat cgttcacgtt
     2641 ggtcgcagca ataaaacgtt tcaccggcag accgagtgac ttcgccagca gacccgccgt
     2701 caaatcgccg aagtttccgc ttggcaccga gacaaccagc tggttgcgcg cttcctgcgg
     2761 cagctgcgca actgcttcaa agtagtagca aatctgcgcc agcaaacggc tgatgttaat
     2821 cgagttagcc gagtttaacc ccagcgccac tttcagttct tcatcatcaa acgcctgctt
     2881 caccagcgcc tgacaggcat cgaaatcgcc gtcgatggca acagtttcga tattgccgcc
     2941 caacgtacag aacagttttt cttgcagtgg actgattttg cctcgtggat agaggataac
     3001 cactttcaca ttcggcaaac cgtagaaagc atgagccact gccgctccgg tatcaccgga
     3061 ggtcgcggtc agaatggtca ctggcttatc gcccgcaata tgggtcagca tttgtgccat
     3121 aaagcgaccg ccgaaatctt taaatgccag cgttggcccg tggaacaatt ccagacaacc
     3181 gacatcgctt tcaacattgg cgaccggagc cgggaaggca aacgccgcgc gcacgcgctc
     3241 ttccaggatt tcctgcggga tttcatcacc aataaacgcc gagaggatct tcgcactgcg
     3301 ggtgacaaaa tccagcttca gcatctcatc aatttcagtc aggctgaatt ccggcaggtc
     3361 atgcggaaaa aacagcccct gatttttgcc caacccctgg gttacggctt gcgcaaagct
     3421 gacctgctcg ttgtgatctt tcagattgta gagtttcatt tagttttcca gtactcgtgc
     3481 gcccgccgta tccagccggc aaatatgaac aaaaccttcc tgattttgca ggtagttctt
     3541 acccaaccag tcggcaacgc gctgggcggt atccggcttg tcacatagag cgaacaaggt
     3601 cgggccggag ccggagatac cgctcgctac cgcgccgatt tccgcgaccg cctgccgcgc
     3661 ctgccggaag ccaggcagta accgttcacg gtagggttca gcgataacat ctttcatcag
     3721 cttcgcggca agctcaggct gacgggaata gcaggcgtga atgaagccag ccagatgtcg
     3781 cccgtgcgca atgcaatcct ggcggcgata ctgcgccggt aaaatagccc gggcttctgc
     3841 cgtcgagact ttaattcccg gatacgccag cacccacagc cactcatcaa accctggcac
     3901 ttgctggctg atgatgtcgt tttcttcgat catcaactgc ataccaccaa gaaaacacgg
     3961 tgccacgttg tcgtaatgaa tgctgccgga gatacgtcct tccagctcgc ccatcaaagc
     4021 cagcaaacgg gtgtcattaa gtggcttgcc gcagtgttca ttcatcgcca tcagcgccgc
     4081 gaccaccgaa caggcgctgg agcctaagcc cgagccgatc ggcatattct tttccagagt
     4141 catcgccact ggaatttgct tgcccagctc caggcaaaaa cgctcccagc actgataaac
     4201 gatattttcc cgtggttctg acggcagctt atcggcaaag cgtccgaggt tgttgagact
     4261 gaatgtctct gccgcctcaa ccgtgactac atctccgagc aatgcaccat caacgggtgt
     4321 caccgccgcc ccgagcacat caaacccgac gctcatattg gcactggaag ccggggcata
     4381 aactttaacc atgtcagact cctaacttcc atgagagggt acgtagcaga tcggcaaaga
     4441 caccagcagc ggtaacgtca ttgcccgcac catatccgcg cagcaccaac ggcagcggct
     4501 gataatagtg gctataaaag gccagggcgt tttcgccatt tttcactttg aacagcggat
     4561 cattcccatc cacttcggca atcttcacgc ggcagacacc atcttcatca atattgccaa
     4621 catagcgcaa aacttttcct tcatcacggg ccttcgccac gcgcgcggca aagagatcgt
     4681 cgagctgtga cagattcgcc ataaaagcgg caacatcacc ctcggcgtta aactctgcgg
     4741 gcagcacagg ttcaatttca atatccgcca gttccagttc acgtcccgtt tcacgagcga
     4801 gaatcaatag tttacgcgcc acatccatac cagaaagatc atctcgcggg tccggttcgg
     4861 tataacccat ttcccgcgcc agcgtggtcg cctcggagaa actcatgcct tcgtctaact
     4921 tgccgaagat ataagaaagc gaacctgaaa gaatgccgga gaacttcacc aattcatcac
     4981 ctgcattgag cagattttgc aggttctcaa taaccggtaa tccagcccca acgttggtgt
     5041 catagaggaa tttacgccgc gatttttccg ccgcataacg caactgatga tagtaatcca
     5101 tcgacgaggt gttggccttt ttgttcggcg tgacaacgtg gaaaccttcg cgcaggaagt
     5161 cggcatattg atccgccact gcctggctgg aggtgcagtc aacaatgacc gggttcagca
     5221 gatgatattc tttcacgagg cgaattaagc gcccgagatt aaacggctct ttggcttgtg
     5281 caagttcttc ctgccagttt tccagattaa ggccatgcac actagtgagc agagccttcg
     5341 agttggcaac gccgcagaca cgtaagtcga tatgtttatt cttcagccag ctttgctgac
     5401 gcttcagttg ctccagcagc gcaccgccaa cgccaccgac gccaatcaca aacacttcga
     5461 taacctgatc ggtattgaac agcatctgat gagtaacgcg cacgccagtg gtcgcatcat
     5521 cgttatttac cacgacagag attgagcgtt cagaagatcc ctgagcaatg gcgacaatgt
     5581 tgatattggc gcgggccagc gcggcaaaga atttcgccga gatcccacgc aaggtgcgca
     5641 taccatcacc taccaccgag ataatggcca gccgttccat caccgccagc ggctccagta
     5701 agccttcttt cagttccagg tagaactctt cctgcattgc ccgttcagcc cgcacacagt
     5761 cgctttgcgg aacgcagaaa ctgatactgt attcggaaga tgattgcgta atcagcacca
     5821 cggaaatacg ggcgcgtgac atcgcggcaa agacgcgcgc cgccatgccg accattcctt
     5881 tcatccccgg gccggaaacg ctgaacattg ccatgttatt cagattggaa atgcccttga
     5941 ccggtaattc gccttcatca cgactggcac caatgagcgt acctggtgct tgaggatttc
     6001 cggtattttt aatcaggcaa gggatctgga actgggcgat gggggtaatg gtgcgggggt
     6061 gaagaacttt agcgccgaag taggaaagct ccatcgcttc ctggtaggac atcgacttca
     6121 acaacctcgc atcgggcacc tgacgcgggt cgcaggtata gaccccgtca acgtccgtcc
     6181 aaatctcgca acaatcggcg cgtaaacagg cagccagcac cgccgcggag tagtcggaac
     6241 cgttgcgtcc aagtaccacc agttcgcctt tttcattacc ggcggtgaag cctgccatca
     6301 gcaccatgtg atcagccgga atacgacttg ccgcaatacg gcgggtggac tcagcaatat
     6361 cgacggtaga ttcgaggtaa tgccccactg ccagtagttt ttcgaccgga tcgataacgg
     6421 taacgttgtg accacgcgct tctaacacgc cggccataat ggcgatcgac attttctcgc
     6481 cacggcaaat cagcgcagcg ttaatgctat ccgggcactg ccctaacaaa ctaatgccat
     6541 gcaggacatg ttttatttgg gcaaattcct ggtcgacgaa agttttcaat tgcgccagcg
     6601 ggaatcccgg ctgggcggcg gcgagtcccg tcaaaagttc ggcaaaaata cgttcggcat
     6661 cgctgatatt gggtaaagca tcctggccgc taatggtttt ttcaatcatc gccaccaggt
     6721 ggttggtgat tttggcgggg gcagagagga cggtggccac ctgcccctgc ctggcattgc
     6781 tttccagaat atcggcaacc cgcagaaaac gttctgcatt tgccactgat gtaccgccga
     6841 acttcaacac tcgcatggtt gttacctcgt tacctttggt cgaaaaaaaa agcccgcact
     6901 gtcaggtgcg ggcttttttc tgtgtttcct gtacgcgtca gcccgcaccg ttacctgtgg
     6961 taatggtgat ggtggtggta atggtggtgc taatgcgttt catggatgtt gtgtactctg
     7021 taatttttat ctgtctgtgc gctatgccta tattggttaa agtatttagt gacctaagtc
     7081 aataaaattt taatttactc atggcaggta accagttcag aagctgctat cagacactct
     7141 ttttttaatc cacacagaga catattgccc gttgcagtca gaatgaaaag ctgaaaaata
     7201 cttactaagg cgttttttat ttggtgatat ttttttcaat atcatgcagc aaacggtgca
     7261 acattgccgt gtctcgttgc tctaaaagcc ccaggcgttg ctgtaaccag tcgaccagtt
     7321 ttatgtcatc tgccactgcc agagtcgtca gcaatgccat gactcgttcg cgtaaagctt
     7381 gcagttgatg ttggtctgcc gttgtgtcac ttttcgccgg ttgttgtatt aatgttgcta
     7441 attgatagca atagaccatc accgcctgcc ccagattgag cgaaggataa tccgccacca
     7501 tcggcacacc agtaagaacg tcagccaacg ctaactcttc gttagtcagc ccggaatctt
     7561 cgcgaccaaa caccagcgcg gcatggctca tccatgaaga tttttcctct aacagcggca
     7621 ccagttctac tggcgtggcg tagtaatgat atttcgcccg actgcgcgca gtggtggcga
     7681 cagtgaaatc gacatcgtgt aacgattcag ccagtgttgg gaaaacttta atattatcaa
     7741 taacatcacc agatccatgt gcgacccagc gggtggctgg ctccaggtgt gcctgactat
     7801 cgacaatccg cagttcgcta aaacccatcg ttttcattgc ccgcgccgct gccccaatat
     7861 tttctgctct ggcgggtgcg accaggataa tcgttatacg catattgcca ctcttcttga
     7921 ccaaataacc gcgaacaggg tgatcactgt caacttatta cgcggtgcga atttacaaat
     7981 tcttaacgta agtcgcagaa aaagcccttt acttagctta aaaaaggcta aactatttcc
     8041 tgactgtact aacggtttaa ttgttaaaaa atgctacata tccttctgtt tacttaggat
     8101 aattttataa aaaataaatc tcgacaattg gattcaccac gtttattagt tgtatgatgc
     8161 aactagttgg attattaaaa taatgtgacg aaagctagca tttagatacg atgatttcat
     8221 caaactgtta acgtgctaca attgaacttg atatatgtca acgaagcgta gttttattgg
     8281 gtgtccggcc cctcttagcc tgttatgttg ctgttaaaat ggttaggatg acagccgttt
     8341 ttgacactgt cgggtcctga gggaaagtac ccacgaccaa gctaatgatg ttgttgacgt
     8401 tgatggaaag tgcatcaaga acgcaattac gtactttagt catgttacgc cgatcatgtt
     8461 aatttgcagc atgcatcagg caggtcaggg acttttgtac ttcctgtttc gatttagttg
     8521 gcaatttagg tagcaaacat gcagaccccg cacattctta tcgttgaaga cgagttggta
     8581 acacgcaaca cgttgaaaag tattttcgaa gcggaaggct atgatgtttt cgaagcgaca
     8641 gatggcgcgg aaatgcatca gatcctctct gaatatgaca tcaacctggt gatcatggat
     8701 atcaatctgc cgggtaagaa cggtcttctg ttagcgcgtg aactgcgcga gcaggcgaat
     8761 gttgcgttga tgttcctgac tggccgtgac aacgaagtcg ataaaattct cggcctcgaa
     8821 atcggtgcag atgactacat caccaaaccg ttcaacccgc gtgaactgac gattcgtgca
     8881 cgcaacctgc tgtcccgtac catgaatctg ggtactgtca gcgaagaacg tcgtagcgtt
     8941 gaaagctaca agttcaatgg ttgggaactg gacatcaaca gccgttcgtt gatcggccct
     9001 gatggcgagc agtacaagct gccgcgcagc gagttccgcg ccatgcttca cttctgtgaa
     9061 aacccaggca aaattcagtc ccgtgctgaa ctgctgaaga aaatgaccgg ccgtgagctg
     9121 aaaccacacg accgtactgt agacgtgacg atccgccgta ttcgtaaaca tttcgaatct
     9181 acgccggata cgccggaaat catcgccacc atccacggtg aaggttatcg cttctgtggt
     9241 gatctggaag attaatcggc tttaccaccg tcaaaaaaaa cggcgctttt tagcgccgtt
     9301 tttatttttc aaccttattt ccagatacgt aattcatcgt ccgttgtaac ttctttactg
     9361 gctttcattt tcggcagtga aaacgcatac cagtcgatat tacgagtcac aaacatcatg
     9421 ccggccagcg ccaccaccag cacactggtt cccaacaaca gcgcgctatc gacagagttg
     9481 agcagtcccc acatcactcc atccagcaac aacagcgcga gggtaaacaa catgctattg
     9541 cgccagcctt tcaataccgc ctgcaaataa ataccgttca ttaacgcccc aatcagactg
     9601 gcgattatcc atgccacggt aaaaccgata tgttcagaaa gcgccagcaa gagcaaataa
     9661 aacatcacca atgaaagccc caccagcaaa tattgcattg ggtgtaaacg ttgcgcggtg
     9721 agcgtttcaa aaacaaagaa cgccataaaa gtcagtgcaa tcagcagaat ggcgtactta
     9781 gtcgcccggt ccgttaattg gtattgatcg gctggcgtcg tcactgcgac gctaaacgcg
     9841 aggaagtttt cccagccggt atcattgcct gaagcaaaac gctcaccgag attattagca
     9901 aaccagctgc tttgccattg cgcctgaaaa cctgactcgc taacttcccg tttggctggc
     9961 aaaaaatcac ctaaaaaact gggatgcggc cagttgctgg ttaaggtcat ttcgctatta
    10021 cgcccggcag gcaccacaga aagatcgccg gtaccgctta aattcagggc catattcagc
    10081 ttcaggttct gcttccgcca gtccccttcg ggtaaaggga tatgcacgcc ctgcccgcct
    10141 tgctctaacc cggtgccggg ttcaatggtc agcgccgttc cgttaacttc aggcgctttc
    10201 accacaccaa taccacgcgc atccccgacg ctaatcacaa taaatggctt gccaaaggtg
    10261 atatttggcg cgtcgagttc gctaagacgc gaaacatcga aatcggcttt tagcgttaaa
    10321 tcactgtgcc agacctgacc ggtataaatc cctatcttgc gttcttccac gttctgattg
    10381 ccatcaacca tcaatgactc aggtaaccaa aaatggataa aacttcgttt ccgctccacg
    10441 gttttatcct cttcctgtac cgtataaagc tcggtcacag gaatagcgat aagcggccca
    10501 acaagttttt gcggcccgct ggtactttgg cgaatcgcat cttccacatc gctacggtaa
    10561 tcggcacgtt cgacaatcac ctgccgaatc agcattatcg gaatcaacaa cagcaatact
    10621 gcaccaaaca gggtggtcat tttccagaac agggaggatt tcaacatagt cttctccttt
    10681 gcaatggcag caggatacga aggctatgtg ggaagaattt gaagctatgt gaagtgacgg
    10741 tgaagtcgaa gcgaggccag cacgccacct tcctgcacgt tgcgcagcgt gacttcgccg
    10801 ttaaacaaac gggcgacctc actgacgaac gccaacccca gaccgctgct tttttgccca
    10861 tttgcacgcg gcaaagagta aaagcgttca aaaatacgtg acagcgcgta gtcaggaata
    10921 ccactaccgg tatccagcac cttaagcgtg acgtgttcct gatccacttc ggcgcttagc
    10981 gttatacgac cgctctcggg ggtaaaatcg atggcgttat ccagtaaatt ccccagcgcc
    11041 tgatccagta acgccggttc agcagcaaca ttaacctcgg tgggcgtaac atgtagagtg
    11101 atgttttttt ctgccaactg cacggtgcgc gcttcgctga cgcggcggaa taatgccgcc
    11161 acatcaacag cagtcaaaac gacttcctga cgattctcca gtcttgcctg gcgtagtaac
    11221 gtttccacca gtgcctgcat tcgcgcattt tgcgtcagaa tgttgtcagt aaaacgagcc
    11281 actacttcag gcggcggacc ttcgcgtaaa atttccgccg cgccacgaat cgccgccagt
    11341 gggcttttta gctcatgggt taacgcataa acatactgct caatatagtt tttcccttcc
    11401 agcttcacgc gcatactttc cagcgcctgc gcgagtttac gcagctcgct actaccgaga
    11461 tcgggaagag gaacgggctt attgtcagtg acggaatcag catagcgagt gagccgggca
    11521 atggagcggt tgatccacca aaccatgcct gcgccaatca ccagtgcgat ccccaacaaa
    11581 atggcgctgg cccataaaat tcgccgctcg ctgcgtttaa tgaccggagc catcgccgcg
    11641 ttcggtttgc ctacgctcaa aacgccaata agccgcgagc cgtccataat cggcgcggca
    11701 acatacatca ccgaactttc gggatcggca ggattttgca acgtgctacg cgcaccatac
    11761 tgaccacgca acgttagcca gacgtcattc cagcgcgaat aatcctgccc aacggcttta
    11821 tttgccgaat cgaacaatac tttgccctgc gcatcggtca tatagacatg atattcattg
    11881 cgcactttgt taatgccacc gatattggcg cgaaacgggc gatgttgtag ctgattaaac
    11941 gcctgagcca gttgcccatg cgttgggtcc ccagagagca aatcgggacg cgccagctcc
    12001 gccagcaacg ttgcggtatc gattaacgtc ccctccgttg ctcttcgcac gcccggttta
    12061 acttctttga caaaaatagc cagtacgaac caggccgcca ccgccaccag taaaaaatag
    12121 cccagcagca accgcatgcc gatacgcatt acaggcccct caggctatat cccatgccgc
    12181 gatgagtatt aatcggagaa agatcggggt tgatggcgcg cagcttggca cgcaacgttt
    12241 taatgtgggt atcgacggtg cgatcgtagg tgtcctgcgc atcttcccac acgctatcca
    12301 ttagttgctg gcgggaccag acgcggcctg gcgacttgag taacgtcttc agcaataaaa
    12361 actcataccg cgtcagcgtt aatggcgtgt caaaccagct gatctgcgcc gcgggttcat
    12421 tcaattcaaa atgaccaata cggatgacgg gagacggcga cgagaacttc ttcacccgac
    12481 gcagtaaggt gcgcaccctg gcgcacactt cgcggggtga aaacggttta gccacatagt
    12541 cgtcagcgcc aatttccagc ccaagcaggc gatcgacctc ttcacttcgg gccgtcagga
    12601 acagtacagg taacgccgga tggagcgcca gtaactggcg gcacaattca aagccgctga
    12661 tatcaggcaa accaacatcg agaatcatga cgtcgggaac ctgctggcga gctttatcca
    12721 gcaccggcaa gcctcgctca aagacctcga cggcaaaacc ttcctgctgc aacatgtaga
    12781 ccagcgtgtc ggctatccct tgctcatctt ccactaacca gaccgtttcc cgttgcataa
    12841 atcgcctctg ttattgccgc cacggcatga caggaaccgc gctaatcgcg tttttcggcg
    12901 aaccttctac aactttgtcg gagtaagcca gataagcgag cgcgttgcgt ttggcgtcat
    12961 aaaagcgcac gacctgtaac gacttaaaga ccagggacgt gcgttttttg aatactacct
    13021 cgccctgagc tttgccgttt ttaatacgat ccgacagttc aatcggcccg acttgctgac
    13081 aagaaatagc cgcatcggag gtatcttccg ccagacccaa tcccccttta ataccacctg
    13141 ttttcgcccg gctcacataa caggtgacat ttttcacatc gggatcgtca aaggcttcca
    13201 caacaatttt gtgatccggg ccgatcattt taaatacggt gtcgaccgaa ccaatctctt
    13261 ctgcatgagc caatggcccc agcattatca ggcttaaaga caggatcaaa tgcttgtatt
    13321 tcatattgtt accattttta tctattacac gagcaattag ttcgtcacgg taaataacat
    13381 attgattaag aacaaaatct caatactttt atttccgtca agccctaaaa catactctac
    13441 taagaaaaaa acactgaatg ctaaaacagc aaaaaatgct attatccaat tacctgatgt
    13501 caggtgctcg ttgttgaaag gatgaggata ttttatggat caggccggca ttattcgcga
    13561 ccttttaatc tggctggaag gtcatctgga tcagcccctg tcgctcgaca atgtagcggc
    13621 gaaagcaggt tattccaagt ggcacttaca gagaatgttt aaagatgtca ctggccatgc
    13681 tattggcgcg tatattcgtg ctcgtcgttt gtcgaaatcg gcggtcgcac tacgcctgac
    13741 tgcgcgtccg attctggaca tcgcgctgca ataccgcttc gactctcaac agacatttac
    13801 ccgcgcattc aagaagcagt ttgcccagac tcctgcactt taccgccgtt ctcctgaatg
    13861 gagcgccttt ggtattcgcc cgccgctacg tctgggtgaa ttcacaatgc cagagcacaa
    13921 atttgtcacc ctggaagata cgccgctgat tggtgttacc cagagctact cctgttcgct
    13981 ggagcaaatc tctgatttcc gccatgaaat gcgttatcag ttctggcacg attttctcgg
    14041 caacgcgccg accattccgc cagtgctcta cggcctgaat gaaacgcgtc cgagtcagga
    14101 taaagacgac gaacaagagg tattctatac caccgcgtta gcccaggatc aggcagatgg
    14161 ctatgtactg acggggcatc cggtgatgct gcagggcggc gaatatgtga tgtttaccta
    14221 tgaaggtctg ggaaccggcg tgcaggagtt tatcctgacg gtatacggaa cgtgcatgcc
    14281 aatgctcaac ctgacgcgcc gtaaaggtca ggatattgag cgatactacc cggcagaaga
    14341 cgccaaagcg ggagatcgcc caattaatct gcgctgtgaa ctgctgattc ctatccgccg
    14401 ttaacgctgc agctcatcta atgcaggggc gtccagatgc gagatgtccc ctgcggtttc
    14461 aacgacccag ccagacgcca gccacaggct ttcctgataa tccacgcgcg aaatagaaca
    14521 gttacgcaga cgtaagcggc gctctgccca tgctggtaat ccgagaatcg tactcaccag
    14581 acatcccagt gcaataccat ggctcaccag cagcggtcgg cttccctgcg gtaagtcccg
    14641 gcaagattcc agtgccgcat tgacgcgatc gctgagctcc tgcattgact cgccttcagg
    14701 aatacgcccg tcaacggtgc cattgaccag ctgccgacgc cagttctctt cttcttcggt
    14761 cagagaatcg atatgtcttt tctccagcac acccatgttt aattcacgca ggcgagaatc
    14821 aaagatgatg tcacagccgc aggcctgggc gatgatttcc gccgtacgcc gggtgcgtcc
    14881 taaatcgcta ctgataatat gcgtaatgcc aagctctttg gcacgggttg ccacctgcat
    14941 cgcctgttgc tcacctttgg cggtcagcgg gctgtcagac tggccctgaa tacgtcgctc
    15001 ggcgttccac tgcgtttcac cgtggcggac taggtatacc tgtaacatgc tttttttccg
    15061 tatactgctg taacgttaat tttctgagta atgctgatta tgcaccaagt tatctgtgcg
    15121 accaccaatc ccgctaaaat tcaggccatt ctgcaggcat ttcacgagat cttcggcgaa
    15181 ggatcctgcc atattgcatc cgttgccgtc gagagcggtg taccggaaca gccctttggc
    15241 agtgaggaaa cgcgcgctgg cgcacgaaat cgggtagcaa atgcccgccg tttacttcca
    15301 gaggctgatt tttgggtggc gattgaagct ggcatcgatg gtgacagcac tttcagttgg
    15361 gtggtgattg aaaacaccag ccagcgcggc gaggcgcgtt cggctacttt gccgttacct
    15421 gcggtgattc tgcagaaagt gcgtgaaggt gaggcgcttg gtcccgtgat gtcgcgttat
    15481 accggtattg atgagattgg tcgtaaggaa ggggcgatcg gcgtgtttac cgccgggaaa
    15541 ctcactcgcg ccagcgtcta tcaccaggcg gtgatcctgg cgctgagtcc gtttcacaat
    15601 gccgtgtatc aggcattgta ggacggataa ggcgttcacg ccgcatccgg cattcggtgc
    15661 acgatgcctg atgcgacgct gccgcgtctt atcaggccta caaaatcaat cgcttttcag
    15721 caacacctct tccagccact ggcgcagctc cacgggcgcg gctttcaggc tgttagatcc
    15781 acgcgtaatc gtcgcgatgc ccgcgccgag ttcatttttt aactcacgct gactcatttc
    15841 gccacgcaac agctcttcga caatacgcac gcgagtcccc aacgcttcgc gctcatctgg
    15901 cgtcagcatc aggtttaaca acggtaaatg gagatcgttt tggtaggcat tcttaagcag
    15961 gtcgacaaaa cgtaaccact cctggtgacg ctgttctgcc atcgctgctg aatagggtga
    16021 ttgttgggcc ataatatgtc gccattgtta gcgggggaag caaaatgcct cccccggttg
    16081 tactcgctaa agagtacgat agcatatcat aaacgtgcgg atcagtaacg acgtccccat
    16141 tccgtggcgc tcatcaacgt cggtttatcc cccatgaaat aacggtagta agcgtcataa
    16201 gccagcacgt tcttcacata accgcgcgtc tcggagaacg gaatactctc gacaaatgcc
    16261 acagcgtcga tacgcccggc gctgttgcca agccaggttc gcacccgtcc aggtccggcg
    16321 ttataagctg ctgaggagaa aatacggtta ttgccaaact gctgataaac atattgcagg
    16381 taactggtgc caatgttgat attcgtttcc ggatccagca attgcccagg gctgctataa
    16441 ccaggaatag agaacatctt caccgtatgg gtcgctgtac caggcataat ctgcatcagg
    16501 ccgctggccc ctaccggtga tttcactttc ggattccagg cgctctcctg acgggcaatc
    16561 gccatcgcat agctttgcgg gatctctttc ccactggtgt aacgtttgaa aagatcgttg
    16621 taagccagcg ggaatcgctc ttccagatga tcccaaagct tcccggcgat cgttgcctga
    16681 acgctaagat cccaccattg gttattgaaa gcataccgag ccagttgagc ctgctctgtt
    16741 tttgacttgc tcttcaccag attggcccac tcgctacgcg cggtgttatc gagattccag
    16801 tacatcaact cgcgcacgcg cgccatctcc ggcccctgag tcagggcgct gtcaacattc
    16861 tgcggcgctt tatcaatctt cagctcatac tcttctccga tgcgttgtgc tgcaaccatc
    16921 gggtagaaac cacgctgttg catgagttga tgcaaaatct ctttcgcttc agcttcacgt
    16981 ccgcgttcca gcaataaatc cacctgccag taacgccatt catctttctc ttttgcttcc
    17041 atcggcagac gcgccagcca ggtattcagg ccgcgacgat cgccggtgcc aagtgccatt
    17101 cgcacacggc gttcaatcaa cgacgtggat tgcgagcgca taatgacgtc atcgcgccat
    17161 ttcgcctgct cgtcggtgac atcgttgccc atcaaacgcc aggcgacgat atcgcgcagc
    17221 tcctgaatct gatcttcatt aagctgctgc acctgggcaa gcgacgggat catcagccgt
    17281 gcattttcag catcctgccg cgccacactg gcaaacgcca ccgctgccat ttgacgggta
    17341 aaatcggtcg ctccggttgt acgcgcgaag gtcagtaccg tattagggtt gttcgccagt
    17401 gaaatgattg ccgaggcgat agtctggtaa tcggcaggca tctgccctgc cagcactgtt
    17461 accaggccag tgttacccgc tttcatcgcc agacggatac gctctaaata cgccagcgga
    17521 tcttgtttac ctgacgcacg ccagacgcta aataacttgt cacaggcgtt aggctggctc
    17581 ttgccggtta gccacagctc tttcgcccct tgccaggctt cttcactctg cccggtgttc
    17641 catttcgcat agtagtaatt acattgcgct tcggtagttc cgggcttttc cgggctaaag
    17701 gctaacaagc cacgccagtc ttcacgccgc gccagttcat tgacgaaacg agattgcagc
    17761 gtgcgagcgg gaggaagcgt ggggttagcg cgaacaaagt tagtgaccgt caccgccggt
    17821 tgattcatca gatcatcggt gatctggcgg tattccaggt agggataaag cggataatcc
    17881 ttcagtccag gcatcatttg ttcgaccaca tccatttgtc gattatccca ggcctgcttg
    17941 atttgcgcgt aacggctacg ctgctcatcc agtgagtcgg ctcgcgccac gctgctgacc
    18001 gtcagcagac agacaccggc agccaacagc cgccaggtaa cttgtttggc tttttccaca
    18061 agcgcatcct ctaagtgtaa atacatcaat gcagcgtcat gccgcgtaat agatttatgc
    18121 taaccagtca ttgccgttta cgccacgtta cggacacttt tttactttta ctgcgaaggt
    18181 gatcgaccaa ctgggattag gcggggaaaa aggctacact tcgcctttga aaactcatca
    18241 tcacgataaa catagaggcg aagtccaacg tggctcaatt cgtttatacc atgcatcgtg
    18301 tcggcaaagt tgttccgccg aaacgtcata ttttgaaaaa catctctctg agtttcttcc
    18361 ctggggcaaa aattggtgtc ctgggtctga acggcgcggg taagtctacc ctgctgcgca
    18421 ttatggcggg cattgataaa gacatcgaag gtgaagcgcg tccgcagcca gacatcaaga
    18481 ttggttacct gccgcaggaa ccgcagctga acccggaaca caccgtgcgt gagtccattg
    18541 aagaagcggt ttctgaagtg gttaacgccc tgaaacgcct ggatgaagtg tatgcgctgt
    18601 acgccgatcc ggatgccgat tttgacaagc tggccgctga acaaggccgt ctggaagaga
    18661 tcattcaggc tcacgacggt cataacctga acgtacagct ggagcgtgcg gcggatgcgc
    18721 tacgtctgcc ggactgggac gcgaaaatcg ctaacctctc cggtggtgag cgtcgtcgcg
    18781 tagcgttgtg ccgcctgctg ctggaaaaac cagacatgct gctgctcgac gaaccgacca
    18841 accacctgga tgccgaatcc gtggcctggc tggaacgctt cctgcacgac ttcgagggca
    18901 ccgtggtggc gattacccac gaccgttact tcctcgataa cgttgcaggc tggatcctcg
    18961 aacttgaccg cggtgaaggt attccgtggg aaggcaacta ctcctcctgg ctggagcaga
    19021 aagatcagcg cctggcgcag gaagcttcac aagaagcggc gcgtcgtaag tcgatcgaga
    19081 aagagctgga gtgggtacgt cagggaacta aaggccgcca gtcgaaaggt aaagcacgtc
    19141 tggcacgctt tgaagagctg aacagcaccg aatatcagaa acgtaacgaa accaacgaac
    19201 tgtttattcc acctggaccg cgtctgggcg ataaagtgct ggaagtcagc aacctgcgta
    19261 aatcctacgg tgatcgcctg ctgattgatg acctgagctt ctcgatcccg aaaggggcaa
    19321 tcgtcgggat catcggtccg aacggcgcgg gtaaatcgac cctgttccgt atgatctctg
    19381 gtcaggaaca gccggacagc ggcaccatca ctttaggtga aacggtgaaa ctggcgtcgg
    19441 ttgatcagtt ccgtgactca atggataaca gcaaaaccgt ttgggaagaa gtttccggcg
    19501 ggctggatat tatgaagatc ggcaacaccg agatgccaag ccgcgcctac gttggccgct
    19561 ttaactttaa aggggttgat cagggtaaac gcgttggtga actttccggt ggtgagcgcg
    19621 gtcgtctgca tctggcgaag ctgctgcagg ttggcggcaa catgctgctg ctcgacgaac
    19681 caaccaacga cctggatatc gaaaccctgc gcgcgctgga aaacgccctg ctggagttcc
    19741 cgggctgtgc gatggttatc tcgcacgacc gttggttcct cgaccgtatc gccacgcaca
    19801 tcctggacta ccaggatgaa ggtaaagttg agttcttcga aggtaacttt actgagtacg
    19861 aagagtacaa gaaacgcacg ctgggcgcag acgcactgga gccgaagcgt atcaagtaca
    19921 agcgtattgc gaagtaatgc gtaaaatgcc ggatgcggcg tgaacgcctt atccggccta
    19981 caaaccaaga aatttcaata cgttgtgaat ctccaggcct gataagacgc ggtaagcgtc
    20041 gcatcaggca ttgagcaccg aatgccggat acggcgtgaa cgccttttcc ggcctacaac
    20101 agccttagta tccgcaattt cgtagcgggt cacccgtgca ggttttttca aaccttgttt
    20161 aatctcgaca gcctcttcca gagaggtttt caactcatca aaaaacttat tttatacctc
    20221 gcctttgagc cgtttcatcg cggttatctc tgctccccca tcatctcccg caccagctcc
    20281 acgcagcgca ggaaacggct gtcataatcg tcctcttcaa cccgcacgaa ttcgatattg
    20341 ttctcttcca gcatctccac cagcaagttc tggaactctt tgcgatccac cgaactgccg
    20401 aggctgcgta aaccatccgc cacccacggc gtgttgttct ccagcaggat caccagatcg
    20461 aaacggtatt catcaatcaa cgcctgtacg aacggatgct cacgcccttc gtactttttg
    20521 cagaacgcct gggtagtgac aaaatcagtg tcaataaacg ccactttatt ggcatatttc
    20581 actgcaaaat caatgtattg tgcgtggccc agcgcgattt tatcgtaatc ggaatactgc
    20641 aatgcgatct catcaccgcc gaggtgtgaa aagacataat cgcgaccata ttcccacgca
    20701 ctggtggtat tgaagatatt ggcaagtttg tttaccaggg tggatttacc gctcgactcg
    20761 ccaccaagga tcgccacggt acgtacaaag aacggcttca cttccgtagg aatatattcc
    20821 cagtagcgga acgggttttc gcggatctgc gcaccgctga tactcataaa ggtacgtttc
    20881 ggatcgacca gcaccgtatc gatccccaga tgttccatat actgtggcgc atcggcttct
    20941 tccgaggtgt agatcaggtc cggctgaatc cccttttcag ccataaactt tttgatgccg
    21001 ttgctccaca catcccagcc gtgcggatac ggctccatgc cctcttcgtt gaaagcatga
    21061 atgcgaatat ttttctgata tttaaaagtt tgtaataacc agcgcagacg atccggcacc
    21121 gtgggctgct gcgacatggc gctgtcttca aacaacgcgc ggtcgcgggt atcgtcaaaa
    21181 cccataatga tatgcagctc gtcaacctgg ctacaggcgc gctggataag gtagatatgt
    21241 ccggtatgca gcgggtagaa cttaccgaat acgacaccaa tggttttctt ctgtcgggga
    21301 aattcaagcc ccaaaaaacg gtgcaacgcc tccagctttt gcgcgctggg gcttttaatt
    21361 ttggcattca gtaactggct taaataccct ttggtcatac cgctggcatc agctacctgc
    21421 tgtagcgtgc agccctgttg cttgatggca gttttcaggt aatcaaatga cgacatatct
    21481 ccctccgtat ctctcattat aagtcgtcga acacgctaag cgcgtcggag agttttttaa
    21541 cgccaaaaat ctgcatccct tccggcgctt ttttcggtac gttagccgcc ggaacaatcg
    21601 cccggcgaaa accgtgtttc gccgcttctg aaattcgttc ctgaccgctg ggcaccgggc
    21661 ggatctcccc tgccagcccg acttcaccaa acaccaccag atcctgtggc agcggtctgt
    21721 cacgcaggct ggaaaccatc gccagcagta acgctaaatc ggcactggtt tcggttacct
    21781 tcacgccgcc gaccacgttc acaaatacat cctgatcggc catttgcaga ccaccgtgac
    21841 ggtgcaagac agccagcagg attgccagac ggttttgttc cagccccact gccacgcggc
    21901 gcggattcgc catcatcgag tgatcgacca gcgcctgaat ctccaccagc aacggacgcg
    21961 ttccttccca caccaccatc acggagctgc cggaggtcac ttcatctccg cgacttaaga
    22021 aaattgccga agggttgctg acttcacgca gcccctgttc ggtcatcgcg aagacgccca
    22081 gctcattcac cgcgccgaag cggtttttat ggctgcgcaa ggtgcgaaaa cgggagtcgg
    22141 catcaccatc cagaagcacc gaacagtcga tacagtgttc cagcacttta gggccagcca
    22201 gcgagccatc tttggttacg tgcccaacca tgacaatcgc cacaccgcgc gttttggcga
    22261 agcgcgtcag ataagccgcc gtttcacgca cctgcgccac gctaccaggc gatgattgta
    22321 catccgccat atgcatcacc tggatcgagt caattaccat cagtttcggt tgctcttctt
    22381 cggcaatcag gcagatctgt tcgatgctgg tttccgacaa catattgaga ttgtcagtcg
    22441 gcaggccaag gcgatgagcg cgcattgcca cctgttgcag cgactcttcg ccagtgacat
    22501 acagcgtttt catctgctgg gccagtttgc acagcgtttg cagtagcagc gtggatttcc
    22561 ccgcacctgg gttaccgcca atcagaatgg cgcttcccgg caccacgccg ccgcctagta
    22621 cgcggtcgaa ctctttaaat ccggtggaaa aacgcggcag ctcttcaagg ctgatatcgg
    22681 agagtttctg gacttttgcc accccggcgc taccggcata gccgctgaga cgctcgttac
    22741 gcgccaccgt tggcgacgca gcaagacgca cctcggtgat ggtgttccag gcatgacagg
    22801 cactgcactg cccctgccag cgcggataat cggccccgca ttcattacaa acaaaggcgc
    22861 gttttggagc ttttgccacg gtgtacctcg ttaatgctgt gccgcccgca ggatggcggg
    22921 cgagcaatta cttctgattc aggctgccag agaggatgca gaataccccc atcaggtcag
    22981 cgtgacggat ggtgacttcc gccttttcat tcacttttgg cttggcatgg taggcaatcc
    23041 ccagccctgc tgctttgatc atcggcaggt cattagctcc atcgccaatc gccacggtct
    23101 gcgccagcgg gatttcatac tcctgcgcga ggcgagtcaa agttttcgct ttgtactgcg
    23161 cgtctacgat gtcgccaatc acattgccgg taaatttacc gtccatgatc tccagttcat
    23221 tggctaccac ggcggtcagg cgcagcttgt cgcgcagata ttcagcaaag aaagtaaagc
    23281 cgccggaggc aatcgccact ttccagccca gcgtttccag cttgagcacc agttgcgtta
    23341 agcctggcat cagcggcaga ttttcacgca cctgttgcag aatattggcg tcagcgcctt
    23401 tcagcgtcgc cacacggctg cgcaggctgg cggtaaaatc gagttcgccg cgcatcgccc
    23461 gttcggttac ttccgccacc atctcgcccg ttccggccag tttggcaatt tcatcaatac
    23521 attcaatctg gatggcggtg gaatccatgt ccatcaccag caaacccggc gtgcgcaggt
    23581 gcgggatttt ccccagcggg gcgacatcca gttgcgcttc atgggccagg cgcgtagccc
    23641 gtgcggtgag tgaacctgcc agacgaatca cctgataatc ttccacgcac caggcggcaa
    23701 caatcaccat cgccgcaccc agtttgctct ggtattgggt cagacgctgt ttatccagcc
    23761 cacgaccata cagcagccag ccgctacgac ctgcgtggta atccagtggc atcacttcat
    23821 caccacttaa tgaaagaggc agacccggcc ataaagagac atcttcaggc aggtcgcacc
    23881 aggtaatgtt aggcattaag gctcctgtaa aatcgttcga agcagggaaa ataacgcatg
    23941 aggctacctt gtatccattg cttctggcaa cattaagtct caaattttca aagggtggaa
    24001 gatggctcgc acaaaactga aattccggct gcatcgggca gtgattgtcc tgttctgtct
    24061 tgccttgtta gtggcactga tgcagggggc gtcatggttt agtcaaaacc accagcgaca
    24121 gcgtaatcca cagctggaag aactggcccg caccctggcg cgtcaggtga cgctgaacgt
    24181 tgcaccgctg atgcgtaccg actcaccaga tgaaaaacgc attcaggcga tcctcgatca
    24241 gttaacggat gaaagccgca tcctcgacgc gggtgtgtat gacgaacaag gcgatcttat
    24301 cgcccgttca ggcgaaagcg tcgaagtgcg cgaccggctg gcgctcgacg gtaaaaaagc
    24361 aggcggctat tttaaccagc agattgtcga gccaattgcg ggtaaaaacg gaccgctcgg
    24421 ctatctgcgc ctgacactcg acacccatac gctcgccacc gaagcccaac aggtggataa
    24481 caccactaac attttacgcc tgatgttgct gctctcactg gcaatcggcg tagtgctgac
    24541 ccgcacgctg ctacagggta aacgcacccg ctggcagcaa tcgcccttcc tgttaaccgc
    24601 cagcaaaccg gtgccggaag aggaagaaag cgagaaaaaa gagtgaccca ttactacaag
    24661 aaaggaaatc gttatgtcca cattacgcct gctcatctct gactcttacg acccgtggtt
    24721 taacctggcg gtggaagagt gtatttttcg ccaaatgccc gccacgcagc gcgttctgtt
    24781 tctctggcgc aatgccgaca cggtagtgat tggtcgcgcg cagaacccgt ggaaagagtg
    24841 taatacccgg cggatggaag aagataacgt ccgcctggcg cggcgcagta gcggtggcgg
    24901 cgcggtgttc cacgatctcg gcaatacctg ctttaccttt atggctggca agccggagta
    24961 cgataaaacc atctctacgt cgattgtgct caatgcgctg aacgcgctcg gcgtcagcgc
    25021 cgaagcgtcc ggacgtaacg atctggtggt gaaaacagtc gaaggcgacc gcaaagtctc
    25081 aggctcggcc tatcgcgaaa ccaaagatcg tggcttccac cacggcactt tgctgctcaa
    25141 tgccgacctt agccgcctgg caaactatct caatccggat aaaaagaaac tggcggcgaa
    25201 aggcattacc tcagtgcgtt cccgcgtgac caacctcacc gagctgctgc cggggatcac
    25261 ccatgagcag gtttgcgagg ccataaccaa ggcctttttc gcccattatg gcgagcgtgt
    25321 agaagcggaa atcatctccc cggacaaaac gccagacttg ccaaacttcg ccgaaacctt
    25381 tgcccgtcag agtagctggg aatggaactt cggtcaggct ccggcattct cgcatctgct
    25441 ggatgaacgc tttagctggg gcggcgtgga actgcatttc gacgttgaaa aaggccatat
    25501 cacccgcgcc caggtgttta ccgacagcct caaccccgcg ccgctggaag ccctcgccgg
    25561 gcgactgcaa ggctgcctgt accgcgcgga tatgctgcaa caagagtgcg aagcgctgtt
    25621 ggttgacttc ccggaccagg aaaaagagct acgggagttg tcgacgtgga tagcgggggc
    25681 ggtaaggtaa ctcgctgatt aaaataccaa aggaagggaa tatgatcgat ctggaaaacc
    25741 aggagcggga gattatcaat ttaatgttaa gccagcgtat ttcctggcta gccgccgtgc
    25801 gtatcaggca taaattatca cttgctgaag taagtaaaat gttaggtatc tcgattaatt
    25861 ctctaaaata gatcgaaaag acggaacggc ttagctcaaa catcaaaagc aaaatgggag
    25921 aaatttatgg ttgtccacca gagttgctga tatgcccttc ctggatgacg gctgaacata
    25981 aatgaataga ttcgtgaata gatacttgaa tgtattttat tttttatcca tatgaaaaat
    26041 aatagtaaat ctataaatca gttgctattg atgaatagat actaaaaagc cggagcagtc
    26101 ccccactccg gcttaacacg acaatcgcct ttcagcgaaa cacaattact ctttatcgcc
    26161 cagcagaacg gattccagtg cgattttgat catgtcgttg aaggtggtct gacgctcagc
    26221 ggcagtggtc tgctcgtgag tgcggatgtg gtcagacaca gtgcagatgg tcagggcttt
    26281 cgcgccaaat tctgcagcga cgccgtagat accagccgct tccatttcca cgccgaggat
    26341 gccgtatttt tccatcacgt cgaacatttc gccgtccgga gagtagaaca ggtcagcgga
    26401 gaacaggtta ccaacgcgag catcaatgcc cagtgcttta gccgcgtcta ccgcgttacg
    26461 caccatgtca aagtcagcga tagcggcaaa gtcatgatct ttaaaacgga tgcggttaac
    26521 tttggaatcg gtgcaggcac ccataccgat aacgacgtcg cgcagtttta cgtgcggcag
    26581 aactgcgcca caggaaccca cgcggataat tttcttcacg ccgaaatcgg tgatcagttc
    26641 tttggtgtag atggagcagg acgggatacc cataccgtga cccattacgg aaattttgcg
    26701 gcctttgtaa gtaccggtga agcccagcat accgcgaacg ttgttcactt cacgggcatc
    26761 ttcaaggaaa gtttcagcaa tatacttcgc acgcagcggg tcgcctggca tcaaaactac
    26821 gtcagcgaaa tcgcccattt ctgcattaat gtgtggggta gccattgttt tatccttatc
    26881 aacatgtttt caccctctcc ctgagggaga gggccggggt gagggcatca agccgcaccc
    26941 attatcagaa catggctttg ccatattcca tatcagaagt accaaaatat tttgccagag
    27001 tctggccgat atccgcgaag gtttcacggt gacccagtga gcccggtttt actttcgggc
    27061 cgtaaaccag taccggaatg tgttcacgcg tgtggtcagt accggtccag gtcggatcac
    27121 aaccgtggtc agcagtgagg atcaggatgt cgtcatcgcg cagcagagac atcagctccg
    27181 gcagacggcg gtcgaacagc tccagaccca cagcataacc ggcgacgtcg cgacggtggc
    27241 cccaggaaga gtcgaagtca acgaagttgg tgaagacgat ggtgttatca cccgcttctt
    27301 tcatctcttt gatggtggcg tcaaacagcg cgtccaggcc agttgctttc actttcttgg
    27361 tgataccgca gttggcgtag atgtctgcaa ttttaccgac agaaaccacc tgaccgtgtt
    27421 tttcatcaac cagtttctgc agcacggtcg gtgctggcgg ctcaacagcc aggtcgtgac
    27481 ggttaccggt acgctggaag ttaccggctt tgtcaccgat aaacggacga gcgataacac
    27541 gaccgatgtt gtagccgccg ttggtcagct cttcacgggc gatttcgcac agttcgtaga
    27601 gtttatccag gccaaaggtt tcttcgtggc aggcaatctg gaacacagag tcagcggagg
    27661 tatagaaaat cggcttgccg gttttcatgt gctcttcgcc cagctgatcc agaatgaccg
    27721 taccggaaga gtggcagtta ccgaggtaac ccggcagatt agcgcgttca accagtttat
    27781 ccagcagctc ttgcgggaag ctgttttcgt gatcggagaa atatccccac tcaaatagaa
    27841 ccgggacacc ggctatttcc cagtgaccag acggggtatc tttaccggat gacatttcgt
    27901 gcgcccatgc gtacgcgccg ataacttcag cgttgccgtc cattcccgcc ggaatgaaac
    27961 cggtagaacc ttcgtgtgct ttcgccagcc ccagacgggt cagatttggc agattgagcg
    28021 gacctttacg accgttatca gcttcgcctt tggcacaagc ttctgcgata tgacccaggg
    28081 tgtcagcccc gacgtcacca aagcgttctg catcttctgt agcgccgatg ccgaatgagt
    28141 ccagcaccat aataaatgca cgtttcatat gttctccgta ctcagtgctt caaatttaaa
    28201 tgatcagatc agtataccgt tattcgctga tacggcgata gacagttggt gtgctttccg
    28261 gtgctttatc ggcaagttta attgccgctt tcaccgcttt cgccgcttcc tgccagctgt
    28321 tttcgtcttt cgcgtggata accgccagcg gacgctgacc gtctacctgg tcgcccaaac
    28381 gcgccatatc agtaaagccg acgctgtaat caatggtgtc agatgcctga cgacgaccgc
    28441 cgcccattgc aaccactgcc atccccagcg cgcgggtatc catttcactg acaaaacctt
    28501 cggtatcagc atagactgct ttcgtcagca tcgctgtcgg cagatacttc gcgtagttct
    28561 caacgaagtc ggttgggcct ttttgtgccg ctaccatacg accaaagact tctgccgctt
    28621 taccgttgtc cagcactgct tgcaatttcg cgcgcgcttc ggcgtcatct ttcgccagtt
    28681 tgccggagat aagcatctcc acgcacagcg ccatcgtgac atcaaacaga cgcgggttac
    28741 gatactcacc cgtcaggaac tgcaccgctt cacgaacttc aaccgcgtta cctgcactgg
    28801 aggccagtac ctgattcatg tcggtgagca gcgcggtggt gcgcacgcca gcgccgttag
    28861 ccacgccaac aatcgcttcg gcaagggctt cagagagttc gtaggtcggc ataaacgcgc
    28921 cgctacccac tttcacgtcc atcaccagcg cgtccagacc ttccgcaagt ttcttcgcca
    28981 gaatagaggc ggtgatcagc gggatggagt ccacggttgc ggtaatatca cgggtcgcgt
    29041 agaaacgttt atccgccgga gccagtgagc tggtctgacc gataatcgcc acgccgacgt
    29101 ctttaataat ttcgcggaaa cggttgtcat ccgggaaaat gtcgaagcca gggatggatt
    29161 ccagtttgtc gagcgtaccg ccagtatgac cgaggccgcg accagagatc atcggaatat
    29221 agccgccgca ggctgcgacc atcggcccca acatcagcga agtcacatcg ccgacgccac
    29281 cagtggaatg tttatcaaca atcgggccat tcagatgcag gcttttccag tcgagaacgg
    29341 ttcctgaatc tcgcatcgcc atggtcagcg agacacgctc aggcattgtc atatcgtgga
    29401 agaaaatggt catcgcgagg gcggcaatct gcccttcgga gatagtgttg tcgcgaatgc
    29461 cgttgataaa gaaacgaatt tcttcatcgc tcagtgcatg accatcacgt tttttacgaa
    29521 taatttcttg tgcgagaaac aaggtaacct cccgaggaaa agagtatgag gcggcttttg
    29581 ccggatgcgt ctacgcctta tccggcctac gggtagatac gtctcgtagg cctgataagc
    29641 gcagcgcatc aggcgtaaag catcttactt agtagctgct ggcgctctta ccgtcaccgt
    29701 ggcccagcgc tttcaacagg cttgccagca ggctggaagc accaaagcgg tagtgacgcg
    29761 catctgccca gtcagcaccg aacagttcat ctgcgatggc gagatatttc tgcgcatctt
    29821 ccgcagtacg cacgccgccc gctggtttga aaccaacggt tttttctacg cccatatcac
    29881 ggatcacttc catcatgatg cgcgcgcttt ccggcgtcgc gttcacagcc actttaccgg
    29941 tagaggtttt gatgaagtcc gcacccgctt tgatggagat ttcagacgct ttacggatca
    30001 gcgcttcgtc tttcagttcg ccggtttcga tgatcacttt cagcagtaca ttcgctgccg
    30061 cgcaagcctc tttacaggct ttcaccaggt caaaaccaac ctgctcgtta cccgccatca
    30121 gcgcgcggta cgggaacaca acgtcaactt catcagcacc gtaggcgatt gccgcacggg
    30181 tttctgccag cgcgatttcg atgtcatcgt taccgtgtgg gaagttggtt accgtagcga
    30241 ttcggatttc cggggtgccc tgctctttca gcgttttgcg agcaatcggg ataaagcgag
    30301 gatagataca gatagcggcg gtattgccga ccggggtttt ggcctgatga cacagagcaa
    30361 ttactttctc gtcggtgtcg tcgtcattca gggtggtcag gtccatcaat ttcagtgcac
    30421 gcaggctgct tgctttcaga tcagtcattt cattctcctg gcttgtcgcc aataaaattc
    30481 accttgcgag tttgttagta ttctaacatc tactccgcaa cacacttcga tatacatcac
    30541 aattaaggaa atctacttac aagtttgcat cactgtaatg cgatctggtt caaataattc
    30601 actttcaaat gaatgcgtca gtggtggcaa acgcatcagg atcaaagtga acatcacgaa
    30661 acttcttaca atggcgcatc accttcggca taaaaggaaa agaagatgcc cacttctcat
    30721 gaaaatgcac tgcaacaacg ttgccagcaa attgtcacca gcccagtgct tagcccggag
    30781 cagaagcgcc attttctggc actggaagca gaaaacaatc tgccttatcc gcagcttcct
    30841 gccgaagccc gccgcgcgct ggatgagggt gtaatctgcg atatgtttga aggtcatgcg
    30901 ccgtacaaac cgcgctatgt cttacccgat tacgcccgtt ttctggcgaa cggttccgaa
    30961 tggctggagc tggaaggcgc gaaagatctt gatgacgcac tctctctgct gaccattctt
    31021 taccaccacg taccgtcggt cacatcgatg ccggtctacc tggggcaact ggatgcgttg
    31081 ttgcaaccat atgttagaat tctaacacaa gacgagatcg atgttcgaat aaaacgtttc
    31141 tggcgttacc tcgacagaac cctgccagac gcctttatgc acgccaatat cggcccgtct
    31201 gattcgccca ttacccgcgc gatcttacgt gcagacgcag agttgaagca ggtttcaccg
    31261 aacctgacct ttatctacga tcctgaaatc acccctgatg acctgctgct ggaagtggcg
    31321 aagaacatct gtgaatgtag caaaccgcac atcgccaacg gtccggtgca tgataaaatt
    31381 ttcacaaaag ggggctacgg gattgtgagc tgttacaact cactgccgct ggcgggtggt
    31441 ggcagcacgc tggtacgcct taacctgaaa gccattgccg agcgcagcga atcgctggat
    31501 gacttcttta cgcgcactct accgcactac tgccagcagc agatcgccat catcgatgcg
    31561 cggtgtgaat tcctctatca acaatcacac ttctttgaga atagcttcct ggtgaaagaa
    31621 gggctgatta accctgaacg ttttgtgcca atgtttggca tgtatgggct ggcggaagcg
    31681 gttaacttgc tgtgtaagaa agaagggatt gccgcgcgct acggtaaaga agccgccgca
    31741 aatgaagtgg gttatcgcat cagcgcgcaa ctggcggagt ttgtcgccaa tacccccgtg
    31801 aaatatggct ggcaaaaacg cgccatgtta cacgcacagt cggggagcag ttccgatatc
    31861 ggcaccacgc cgggcgcgcg tttgccgtat ggtgatgagc cagatccgat cacccatctg
    31921 caaactgtcg ccccgcatca tgcttattat tattccggca tcagcgacat tctgacgctc
    31981 gacgaaacca tcaaacgtaa tccgcaggcg ctggtacagc tttgcctcgg tgcctttaaa
    32041 gccggaatgc gtgaatttac tgccaatgtc agcggtaacg atctggttcg cgttaccggt
    32101 tatatggtgc gtttgtcgga tttagaaaaa tatcgcgccg aaggttcacg caccaacacc
    32161 acctggctgg gcgaagaagc cgcacgcaac actcgtattc tggaacgcca gccgcgcgtg
    32221 ataagccatg aacagcagat gcgctttagt cagtaagatt atccccttct cctgcgttga
    32281 cgggccaggc agtcgtctgg ctctgttttt gcagggctgc aatctgcgct gcaaaaactg
    32341 tcacaatccg tggacgatgg gacgttgcaa tgactgtggg gagtgcgtgc cacagtgtcc
    32401 gcatcaggcg ttgcagattg ttgacggcaa agtggtgtgg aacgctgtgg tttgcgagca
    32461 gtgtgatacc tgcctgaaga ggtgtccgca acatgccacg ccaatggcgc aatccatgag
    32521 cgtggacgaa gtgcttagcc atgtccgcaa agcggtgctg tttattgaag ggataacggt
    32581 aagcggcggc gaagccacga cccaactgcc gtttgtagtg gcgctgttta ctgctatcaa
    32641 aaacgatccg caactgcgcc acctcacctg cctggtggac agtaacggca tgttgagcga
    32701 aaccggctgg gaaaaattac tcccggtgtg tgacggcgca atgctcgatc tcaaagcgtg
    32761 ggggagcgaa tgtcatcaac acttaaccgg acgcgataat cagcagatta agcgcagcat
    32821 ctgtttgctt gcagagcgcg gcaagctggc ggaactgcgt ttgctggtaa ttccagacca
    32881 ggtggattat ttgcatcaca tcgatgagct ggcgacgttt atcaagagac ttggcgatgt
    32941 tccggttcgc ctgaatgcgt ttcatgccca cggcgtgtat ggcgaggcgc aaagctgggc
    33001 gagcgccacg ccggaagacg ttgagccgtt ggctgatgcg ttaaaggtgc gcggggtgag
    33061 ccggttgata tttccggcgc tctatttgtg acatgccgga tacggcctac ggttcggcac
    33121 agacttgtag gcatgataag acgcgacaag cgtcgcatca ggcattatgc gccgactgcc
    33181 ggatgcggcg tgaacgcctt atccggccta cggttcggca cagacttgta ggcatgataa
    33241 gacgcgtcag cgtcgcatca ggcattgtgc gccaactgcc ggatgcggcg tgaacgcctt
    33301 atccggccta cggttcggca cagacttgta ggcatgataa gacgcgtcaa gcgtcgcatc
    33361 aggcattgtg cgccaactgc cggatgcggc gtgaacgcct tatccggcct acggttcggc
    33421 acaaaccagt aggcatgata agacgcgtca agcgtcgcat caggcattgt gcgccaactg
    33481 ccggatgcgg cgtgaacgcc ttatccggcc tacggttcgg cacaaacctg taggcatgat
    33541 aagacgcgtt aagcgtcgca tcaggcatta tgcgccgact gccggatgct gcctacgaag
    33601 ggtaggctac ccactaacgc taaacaacgc atacgtatta ttaagcaaca cttcggcaat
    33661 ctcatcttct ggttccgggc gcaactcgca aagcacgtca aacacccgca cagcctgttc
    33721 cggtcggtta ggctgcccct gaaagccgtt gagcggcata tccggcgcgt cggtttccag
    33781 caacaacgat gccagcggta attttgcgat gacatcgcgg gttttactgg cgcgtggata
    33841 ggtgatagta ccgcctacgc cgattttgta gcccagctgt acaaaccgct cggcctgttg
    33901 caggctgccg gaaaaaccgt gaaccacgcc agtgcacggt aaatcgtgac gtttaagatg
    33961 catcgccagt ttgtcgtgta tacgccggga atgaaggatc accggcagat cgtagcgttt
    34021 cgccagtttc agctgttcgt cgagtaacca ctgctgcctc tcaaattgcg gatcgtcgcc
    34081 gaagagatcc agaccgatct cccccaccgc caccaccttc gccggacgcc tttccagcgc
    34141 ctgctgtagc tgatcaagag acacatcgct atgtttttcc aacatccccg gatgcaaccc
    34201 caatgcggca tacagcggtt gatatttttc cgctaacgcc tgcacgcggg caaaattttc
    34261 cgcctctgtc gccggaacaa tgatcttgcc tacgcccgct tgtgccgcgc gttgcaggct
    34321 ggcctcttca tcgccactaa acggcgggaa atcaaaatgg cagtgggtat cgataaaacg
    34381 gcaaatcaag ccagatcctc attgttaaat gtcgggtcgt tcgcctgcgg agcatcgctc
    34441 acttcggcaa ccagcgtatc gttggcgact ggcgcaggcg gaataacgat cgattccggc
    34501 gtcaccactg gcaccagatg gcgggtaagc ggcgcttttt cagttaatag cttgccaacc
    34561 gtggcgagga aataacgacc gcataaacgc ccaagtttat agtcttcacg cagcgccgga
    34621 atccgactgc caagcgcgat actatgtaat ggcttcggcg gataaatttc gaatatccgc
    34681 agcttgcccg gtggtttctc aataaattgc tgaatgtcac gatagctggt ttcatgatgc
    34741 tgcaccagat tgaccagcgg ctgcaggcta ctgtcaccca gccagcgttc catgcgtttg
    34801 aaccactgcg gcgtgtagta catttgtgac ggcacagtgc gaatgacgac caacgtttta
    34861 gcgccctgcc ttgccgcctc tttaaccgga atcgcatcac tgatcccgcc atccaggtag
    34921 ttaatgcctt ccagcgacac tccgctacga taaaagccag gtatcgcact ggaggcgcga
    34981 atcacatcca gccagttttg tttggttggt aaaaagtaat taggcgcgta gtcatcctga
    35041 cgacaggcgc acatataaaa cgatttgccg ctgtcaaaca accgcgcggc ggtatccatt
    35101 tgcaacggca tctggcttgc ggtggcctcc accagccagt cgagatcgat aagatttcct
    35161 ccacggacaa agcgcaatgg atcgaaaaat tcgcgttttg tggtatagcg catgatgact
    35221 ttgcgcgcgt aaccgggctg attgcagata aacgccgaga ggttctgcgc cccggcagat
    35281 gtgccgagat aaagatcgaa aggattaaac tgcgcgcgca taaactcatc cagcacgcca
    35341 gccgtgaaaa ttccacgctg tccgccgcct tcgcacacca gagctattcg tccaggctgg
    35401 aacggcctta gcgacaacgg cgcaatatta ccaagcgtta caggtattcg ctgccccacc
    35461 tctgccttcc tgtttttgtt gtgaaagaat gacaaagtaa cgcaatttat tattcgctaa
    35521 aaccgcaaaa gccagtccgc tggactggct ttggataaaa ttttcttaat ggtctgaaat
    35581 ctagggtcgt tttcggccca tgaacaaact caccaggaac agaataatcc cgacgacaaa
    35641 gacaatttta gctgcgcctg cagcggtacc ggccagacca ccaaacccaa gtgcggcggc
    35701 gattaacgcg ataaccagaa atatgatgcc ccaacgaaac ataagcgtct cctttaccat
    35761 agttaatgtc accgcttgat atgagcgtga aaatcactca atgcgatatt tttagtgtgg
    35821 tgcacatcgc gcctcccgac aaagttcggg aggacgaatt acgacgaatt acttagtttt
    35881 cagatcattt ttaacgcttt tcacaccatc taccgctttg gcgatacttt cagcacggtc
    35941 gctctgtgcc tgggaatcga cggtaccgga gagctgaacc acgccgtcgg tggtttcaac
    36001 tttcacatga cgggaaggga cgatatcgtc cgccagcagt ttggctttga tttcactggt
    36061 ggtggcggtg tcacccgcgt agcccttcac agagccttct ttagcgtcgc gaacgtgcag
    36121 tttgtcgctg acagaggtca ccccttcaac gcctttcgcc actttcactg cctcttcggc
    36181 ctgagcctgg ctttcaacga aaccgctcag ggtcacgact ttttgatcgg ttttgacaga
    36241 gatatcggtg ctcttgatgt tgtcatgatc caccagcgcc gccttcactt tcgcggtgat
    36301 ggcgctgtca tccatgaaat taccgacttt attcatagag ctatcgactt tttgccctgc
    36361 gctttcattg gtagtctgtg cgttgttttc cgcgtaggca gagccggtcg cgacggcaga
    36421 ggtcaacatt acagccagca gagttttcga aatcttcagt cttgtcatag tcatcgattt
    36481 attcctgtat gtttgctcgt aatttgagcc tggcaacacg aggttgcatt gctgaatagg
    36541 gagagacttc accctctaca gaagtcaatg gtcgccatca caaaagcgat gggtgatgaa
    36601 taacgaccat tacagcctct gaatcagtta ttaatatcgg tagaatgaca atcgacggct
    36661 ttagatactg atatctacgc attgaacggt atttaacgcc gtcagaaatg tcattacttt
    36721 gttaaatata gatcacaatt ttgaaaccgc tcgggatatc agcgaaaaca taagcaaaag
    36781 tgaatgtttt aagaacattc cgtaagcggc taataaggaa gggaaattga cagggcgcag
    36841 cggctaccgc gccctggagg caagaattaa tgctcgcggg tctggtggaa ctgaacgtcg
    36901 ggataacgtt cctgcgccag gcgcaggttg accatgctgg tagcgatgta agcgaggtta
    36961 tcgccgccat caagcgccag ttggctttcg ttcttacgct tgaactcttc gaatttcttc
    37021 gcgtccgcac attctaccca gcgggcagtg gcaacgttga ctgattcata tactgcttca
    37081 acgctgtatt cgctcttcag gcgcgatacc accacatcaa actgcagcac accaacagca
    37141 ccaacgatca aatcgttgtt ggagatcgga cggaacacct gcaccgcgcc ctcttcggaa
    37201 agctgtacca gccctttgag cagctgtttt tgcttcagcg gatctttcag gcggatacga
    37261 cggaacagtt ctggtgcgaa gttcggaata ccggtgaact tcatcatctc accctgggta
    37321 aaggtgtcgc cgatctgaat agtgccgtgg ttgtgcaggc cgaggatatc gcccggatac
    37381 gcttcttcaa cgtgcgaacg gtcgcccgcc ataaaggtca gcgcgtcgga gataaccaca
    37441 tctttcgcag tacgcacctg gcgcagcttc atgccttttt catatttacc ggatactacg
    37501 cgcataaacg ccacgcggtc gcggtgtttc gggtccatgt tggcctgaat tttaaatacg
    37561 aagccggtaa atttgtcttc gctcgcctct acggtacggg tatcagtctg acgcggcatc
    37621 ggcgcaggag cccactccac caggccatcc agcatatgat caacgccgaa gttacccagc
    37681 gcagtaccga agaataccgg agtgatttcg cccgcaagga acaattcttt gtcgaactcg
    37741 ttagacgcgc ctttcaccag ttccagttcg tcacgtagct gctgtgccag atcttcacca
    37801 accgcagcgt cgagatccgg gttattcagc cctttaacaa tacggacttc ctgaatcgtg
    37861 tggcctttac cgctctgata gagataggtt tcgtctttat aaaggtggta aacgccttta
    37921 aacagcttgc cgcagccaat cggccaggtg atcggtgcgc agccgatttt cagctcgttc
    37981 tcaacttcat cgagcagttc catcgggtcg cggatatcac ggtcaagttt gttcataaag
    38041 gtgaggatcg gcgtgtcgcg cagacgggta acttccatca gcttacgggt acgatcttca
    38101 acaccttttg cagcgtcgat aaccatcagg cagcagtcca ccgccgtcag ggtacgatag
    38161 gtatcttccg agaagtcttc gtgccccggg gtgtcgagca ggttaaccag gcaatcgtga
    38221 tacggaaact gcatcacaga cgtagtaatg gagatcccac gctgcttttc catctccatc
    38281 cagtccgact tagcgtgctg gttggaacca cggcctttta ctgtaccggc ggtctgaatg
    38341 gcctgtccga acagcagcac cttctcggtg atggtagtct taccggcatc cgggtgagaa
    38401 ataatggcaa aagtgcggcg cttcgccacc tcttgcaaat aaggagacaa cgtcataatt
    38461 ctttcttctt gagtaagcgg catcgcgccg cgcttgttga aaacgaaaaa ttgcggctat
    38521 tttacccatc aacggggggg aggcaatcag tgtttacaca ggagctgctc cagttcgtgc
    38581 aacgaagaaa cggtccaggt gggcgcgatg ccttctggtt gctcgcgatt gtgcgcattc
    38641 agccagcagg tcgcaagccc ggcgttgatg ccaccgagaa tatcggactc ggcagtgtcg
    38701 ccaaccatca gcacgcgtga acggtcagga ttgcccgcct gttccagcgc ataatcgaaa
    38761 attttcttat tcggtttggc aacgccaact tcttcggaaa tcaccagcaa atcgaagtaa
    38821 tcacgcaggc ccgtgcgttc cagacgcact tgctgcaagg cgctaaagcc gttggtgatg
    38881 atgccgattt tggcgttgcc acgaatggcg ttaagcagag aaaccgcgcc cggcagcggc
    38941 gtgcagattt ccgccatcgc attaataaag gcatcgttaa gcaagcctgc ttcaactttc
    39001 agccgttcgg cccagctctc aaaacgcccg tgctgaagct gtaatgaagt gatcgcgccg
    39061 ttttgataat ccacccacag tggcttgtta acggcctgat agtcctgaaa atcttcagcg
    39121 gtaaaggtga cgctgtaatc aagaaacatc cgctgcaggc cggtgaatga gtcaaaggta
    39181 aacagcgttt catcggcatc aaagaaaatc cagtcccact tcattattcc accttgtctt
    39241 acatactgat tggcaacgcc atgatgatgg cgtcttcgcg accgtccgtg gtggggtagt
    39301 aattgcggcg aatcgtcgcc tcgttaaagc ctaaactttc gtacagggca atggcggcag
    39361 catttgaggc acggacttcc agccatagtg tcgccacgcc gcgtttttcc agttcgtcga
    39421 tcagatgttc cagcagcacc cttcccaatc cctgacgctg atagtcagga tcgaccgcaa
    39481 tattgaacaa tgtagcttca tccagcacca cttgcgtaat cgcaaacgcc gccattttgc
    39541 cgttttgcgt taactgaaag ttgagataac gctcgccctg gttgctggca aaggtttttt
    39601 cactccacgg aaaggcgtgg gcgcgttgtt caatgtggta agccgctggt aaatcagtcg
    39661 tttcgaggga agaaatcgtg ttcatatgtg caaatctgtt gccataacgc ggcgcgtgcc
    39721 gttgggtttg cccgtaattc ggtgagcgcc ggtgatgcca cctgagcgcc ttccagtgat
    39781 agcggttcgt cagtacccaa ccgccagctg ttgcagcgac tgccttgcgg cagcatcgcg
    39841 attttttctg gcgtcagttg cagcacttga tcggggctga cggttaatgc gcgcagaaca
    39901 tcgctcacta aaggatcggt cagggcggga agatcgtttg ccaccatcac cagacggacg
    39961 tgtgccggga tcgcaatggc aatctccccc tgcaacgcgc cagggcgacg cagcgaccac
    40021 tgggtaatgc ccagttgctg taactgccag tctcgtcggg atgtcatagc gaatcgctcc
    40081 tgttgtcagg ggcgcaaata tagcaaattc gtcgataccg cgccaacata tggctataat
    40141 cgccgccagt atcaattgag gagcattcca tgtctgcatt taccccggca agtgaagtct
    40201 tgctgcgtca cagtgatgat ttcgaacaaa gccgtattct gtttgccgga gacttacagg
    40261 atgacctgcc cgcgcgttta gataccgcgg ccagccgtgc tcatacccag caattccacc
    40321 actggcaggt attaagccgc cagatggggg ataacgcccg tttcagtctg gtcgccacgg
    40381 tggatgacgt cgcagattgc gatatgctga tttactactg gccgaagaac aaaccggaag
    40441 cccagttcca gttgatgaat ttactttctc tgctgccagt ggggacagat atttttgtcg
    40501 ttggcgagaa ccgcagcggc gtgcgcagcg ccgagcagat gctggcagat tatgcgccgt
    40561 tgaataaagt cgacagcgct cgtcgctgtg gcctctattt tggtcgtctg gaaaaacagc
    40621 cggtatttga tgccgataaa ttctggggcg aatacagcgt cgatggcctg acggtcaaaa
    40681 cgctgcctgg cgtgtttagc cgcgacggtc tggatgtcgg tagccagttg ctgctctcga
    40741 cgttaactcc gcacacgaaa ggtaaagtgc tggatgtcgg ctgtggcgcg ggggtgcttt
    40801 cagttgcctt tgcgcgccat tcgccgaaaa ttcgtctcac cttgtgcgat gtctctgcgc
    40861 cagcggtaga agccagccgc gcaacacttg cggccaactg tgttgaaggt gaagtctttg
    40921 ccagcaacgt cttttccgag gtgaaaggtc gttttgatat gatcatctcc aacccgccgt
    40981 tccacgatgg gatgcaaacc agcctggatg cggcgcaaac gctgattcgc ggcgcggtgc
    41041 gtcatcttaa tagcggcggc gagctgcgaa ttgtagcgaa cgccttcctg ccttacccgg
    41101 acgtgctgga tgagacattt ggcttccatg aagtgattgc gcaaaccggg cgtttcaagg
    41161 tgtatcgcgc cattatgacc cgccaggcga agaaaggtta attatctcgc cgaataccgt
    41221 gtcggatgcg gcgtgaacgc cttatccgac ctacaaaacc tgcacgttag cccttcgtag
    41281 gccagataag acgcgccagc gtcgcatctg gcatctgccc ccgcatgcct accaaacctg
    41341 cacgctagtt tcctgatgga catttttcca gcaattacac ctccggcgat aattaactat
    41401 tgacgaaaag ctgaaaacca ctagaatgcg cctccgtggt agcaattctt tttaagaatt
    41461 gatggtatgc gaaggtggcg gaattggtag acgcgctagc ttcaggtgtt agtgtcctta
    41521 cggacgtggg ggttcaagtc ccccccctcg caccaattat ctttacttcc tttcttgttt
    41581 cttccttgat ttttcatccc tgattcaaat tcattatcat cagcatgatg gcagcaataa
    41641 gcgccaggca cgatggtagc aaaacgtaat gccgtaactg tttgtgatac agcatcaccg
    41701 gcgtcaatac taatcccatc agaatgacaa tccgccactg atccggcgat aaatccgcat
    41761 acatcagccc tgcaatcagc aatcccggca gcaacactcc ccagccactg cccagcgtac
    41821 gttgcaacat ggtttcatct ctttcattga taatgataac caatatcata tgataatttt
    41881 tatcatttgc aagccagaga aatcccttgc tatcgggtaa acctatcgct aggattagca
    41941 atcattatca tttagattac tatcccgatt atggcctatc gttccgcacc gctctatgaa
    42001 gatatcatct ggcgaacgca tctccagccg caggatgctg ggcttgcaca agccgtacgt
    42061 gcgacgatcg ccaaacatcg tgaacatttg ctcgagttta tccgcctgga tgaacccgct
    42121 ccgcttaacg ccatgacgct ggcgcaatgg tcatcaccga atgcgctaag ttctctactc
    42181 gcggtctatt ccgatcatat ctatcgcaac caaccaacga tgatccgcga gaacaaaccg
    42241 ctgatctcac tatgggcaca attgtatatc ggcctgatgg tgccaccatt aatgctggcg
    42301 ctactgacgc aggaaaaggc attagatgtg tcgccggaac atttccatgc tgagtttcac
    42361 gaaaccggac gcgtcgcctg tttctgggtc gatgtgtgtg aagataaaaa cgcaacacca
    42421 cattcgccgc agcagcgaat ggaaacgtta atcagccagg cgctggttcc ggttgtgcag
    42481 gcactagaag cgaccggaga gatcaacggc aaacttatct ggagtaatac cggttatttg
    42541 atcaactggt atctcactga gatgaagcaa ctgctcggcg aggcgacggt tgaatcgctg
    42601 cgccatgccc tcttttttga gaaaacgctc acgaacggtg aagataatcc actctggcgt
    42661 accgtcgtgc tgcgcgatgg cctgctggtg cgccgaacct gctgccagcg ttatcgctta
    42721 ccggatgtgc agcaatgtgg cgattgtacg ctgaaatgaa agcactgccg gggaagtaaa
    42781 cccggcatca tgcggattaa tagggatgca acacattact tgcttcgcaa tgccgcatac
    42841 ataacagaat atctgcggcc tccaacaacc ctgcatcaga actgacgccc agtttcgaca
    42901 tcacattaaa tttatgtgca cggatcgttt tgatattgcg tttaagctgc tcggcaattt
    42961 gtgtcattga gtagccacgc gacataaagc gcaatatttc tctctccgtc gggcttagcg
    43021 tccggctttg gttaatgtac cactgattgt tcagtcggtc ggtcgcctga cgtacaccat
    43081 ttaatgacaa aaagagttcc tgatgaaaaa tctccagcgt tgacgcttta cttaatacac
    43141 cgtccagcgg tgatggcgac aatgaaccaa tcagccgagc ttcaatatca tcatccgcaa
    43201 taactaaacg ccgggtacgc ggaaacttaa tcgccagttc agtcaggcaa gataatccct
    43261 ctctgcgctc acttctcatg gcagaaaaag aaaaaatgac cgccgcaaat gagatccgcg
    43321 acatggcatc ctgaaatgac tcctgactgg tataggtatg gagcttataa gggttacccg
    43381 caaactttct gaataaactc tcaataccga tactgctcat gacgcatttt tctatgagtg
    43441 cgacatttct cttcttaatt cggctgtgtt ccattctgcg gcatctccat aaacagaagg
    43501 caagttaagt tctcttgcac cctgcgcgct atttatccat gcatacattt cagcgttgct
    43561 gtgaattgcc
//
LOCUS       DADMKF010000014        43275 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.014, whole genome shotgun sequence.
ACCESSION   DADMKF010000014 DADMKF010000000
VERSION     DADMKF010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 43275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43275
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.014"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            59..577
                     /locus_tag="JNC24_000726"
     CDS             59..577
                     /locus_tag="JNC24_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HAY9157555.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQVGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
     gene            593..1132
                     /locus_tag="JNC24_000727"
     CDS             593..1132
                     /locus_tag="JNC24_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9157556.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEATADKKRDQSYE
                     DELRSLEIQKQKLTLAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(1227..2804)
                     /gene="guaA"
                     /locus_tag="JNC24_000728"
     CDS             complement(1227..2804)
                     /gene="guaA"
                     /locus_tag="JNC24_000728"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAY9157557.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPVFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWARLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(2873..4339)
                     /gene="guaB"
                     /locus_tag="JNC24_000729"
     CDS             complement(2873..4339)
                     /gene="guaB"
                     /locus_tag="JNC24_000729"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004159092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HAY9157558.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            4501..5871
                     /gene="xseA"
                     /locus_tag="JNC24_000730"
     CDS             4501..5871
                     /gene="xseA"
                     /locus_tag="JNC24_000730"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HAY9157559.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDQQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTAVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     TRQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRTGQQQQRLTQRLNQQNPQPKIHRAQTRIQQLEYRLAETLRVQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKAGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            complement(5868..6083)
                     /locus_tag="JNC24_000731"
     CDS             complement(5868..6083)
                     /locus_tag="JNC24_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9157560.1"
                     /translation="MELHCPQCQHVLDQDNGHARCPSCGEIIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(6152..7624)
                     /gene="der"
                     /locus_tag="JNC24_000732"
     CDS             complement(6152..7624)
                     /gene="der"
                     /locus_tag="JNC24_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HAY9157561.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLERVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDTNVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            complement(7742..8920)
                     /gene="bamB"
                     /locus_tag="JNC24_000733"
     CDS             complement(7742..8920)
                     /gene="bamB"
                     /locus_tag="JNC24_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HAY9157562.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWTQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            complement(8931..9551)
                     /locus_tag="JNC24_000734"
     CDS             complement(8931..9551)
                     /locus_tag="JNC24_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgM family protein"
                     /protein_id="HAY9157563.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGKALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            complement(9569..10843)
                     /gene="hisS"
                     /locus_tag="JNC24_000735"
     CDS             complement(9569..10843)
                     /gene="hisS"
                     /locus_tag="JNC24_000735"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HAY9157564.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGIFEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELPGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            complement(10954..12072)
                     /gene="ispG"
                     /locus_tag="JNC24_000736"
                     /gene_synonym="gcpE"
     CDS             complement(10954..12072)
                     /gene="ispG"
                     /locus_tag="JNC24_000736"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HAY9157565.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQRVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            complement(12099..13112)
                     /gene="rodZ"
                     /locus_tag="JNC24_000737"
     CDS             complement(12099..13112)
                     /gene="rodZ"
                     /locus_tag="JNC24_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HAY9157566.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPVPTAATTPDGAAPLPTDQAGVTTPAADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            complement(13397..14551)
                     /gene="trmG/rlmN"
                     /locus_tag="JNC24_000738"
     CDS             complement(13397..14551)
                     /gene="trmG/rlmN"
                     /locus_tag="JNC24_000738"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HAY9157567.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKPFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            complement(14701..15132)
                     /gene="ndk"
                     /locus_tag="JNC24_000739"
     CDS             complement(14701..15132)
                     /gene="ndk"
                     /locus_tag="JNC24_000739"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HAY9157568.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            complement(15281..17593)
                     /gene="pbpC"
                     /locus_tag="JNC24_000740"
     CDS             complement(15281..17593)
                     /gene="pbpC"
                     /locus_tag="JNC24_000740"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HAY9157569.1"
                     /translation="MPRLLTKRGCWITLAAAPFLLFLAAWGADKLWPLPLHEVNPARV
                     VVAQDGTPLWRFADADGIWRYPVTIEDVSPRYLEALINYEDRWFWKHPGVNPFSVARA
                     AWQDLTSGRVISGGSTLTMQVARLLDPHPKTFGGKIRQLWRALQLEWHLSKREILTLY
                     LNRAPFGGTLQGIGAASWAYLGKSPANLSYSEAAMLAVLPQAPSRLRPDRWPERAEAA
                     RNKVLERMAAQGVWSREQVKESREEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTL
                     DAGLQRRLEELAQNWKGRLPPRSSLAMIVVDHTDMRVRGWVGSVDLNDDSRFGHVDMV
                     NAIRSPGSVLKPFVYGLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPISMSEA
                     LVRSLNLPAVQVLEAYGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAA
                     AYTAFARHGKAGKLRLQPDDPLLERPLMSSGAAWIIRRIMADEAQPLPDSALPRVAPL
                     AWKTGTSYGYRDAWAIGVNARYVIGIWTGRPDGTPVVGQFGFASAVPLLNQVNNILLS
                     RSANLPEDPRPNSVTRGVICWPGGQSLPEGDGNCRRRLATWLLDGSQPPTLLLSEQEG
                     INGIRFPIWLDDKGQRVAADCPQAKQEMVNVWPLPLEPWLPASERRAVRLPPASTICP
                     PYGHDAQLPLQLTGVRDGAIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRN
                     VTLHLTDKGDYQLLVMDDVGQIATVKFVMQ"
     gene            complement(17594..22555)
                     /gene="yfhM"
                     /locus_tag="JNC24_000741"
     CDS             complement(17594..22555)
                     /gene="yfhM"
                     /locus_tag="JNC24_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha2-macroglobulin"
                     /protein_id="HAY9157570.1"
                     /translation="MKKLRVAACMLMLALAGCDNNDNAPTAVKKDAPSEVTKAASSEN
                     ASSAKLSVPERQKLAQQSAGKVLTLLDLSEVQLDGAATLVLTFSIPLDPDQDFSRVIH
                     VVDKKSGKVDGAWELSDNLKELRLRHLEPKRDLIVTIGKEVKALNNATFSKDYEKTIT
                     TRDIQPSVGFASRGSLLPGKVVEGLPVMALNVNNVDVNFFRVKPESLPAFISQWEYRN
                     SLANWQSDKLLQMADLVYTGRFDLNPARNTREKLLLPLGDIKPLQQAGVYLAVMNQAG
                     RYDYSNPATLFTLSDIGVSAHRYHNRLDIFTQSLENGAAQQGIEVSLLNEKGQTLTQA
                     TSDAQGHVQLENDKNAALLLARKDGQTTLLDLKLPALDLAEFNIAGAPGYSKQFFMFG
                     PRDLYRPGETVILNGLLRDADGKALPDQPIKLDVIKPDGQVLRNVVSQPENGLYHFTW
                     PLDSNAATGMWHIRANTGDNQYRMWDFHVEDFMPERMALNLTGEKTPLTPNDEVKFSV
                     VGYYLYGAPANGNTLQGQLFLRPLREAVSALPGFEFGDIAAENLSRTLDEVQLTLDDK
                     GRGEVSTESQWKETHSPLQVIFQGSLLESGGRPVTRRAEQAIWPADALPGLRPQFALK
                     SVYDYRTDSTVKQPIVDEGSNAAFDIVYSDAQGVKKAVSGLQVRLIRERRDYYWNWSE
                     DEGWQSQFDQKDLIENEQTLDLQADETGKVSFPVEWGAYRLEVKAPNEAVSSVRFWAG
                     YSWQDNSDGSGAVRPDRVTLKLDKASYRPGDTIKLHIAAPTAGKGYAMVESSEGPLWW
                     QEIDVPAQGLDLTIPVDKTWNRHDLYLSTLVVRPGDKSRSATPKRAVGVLHLPLGDEN
                     RRLDLALETPAKMRPNQPLTVKIKASTKNGEKPKQVNVLVSAVDSGVLNITDYVTPDP
                     WQAFFGQKRYGADIYDIYGQVIEGQGRLAALRFGGDGDELKRGGKPPVNHVNIVAQQA
                     LSVTLNEQGEGSVTLPIGDFNGELRVMAQAWTADDFGSNESKVIVAAPVIAELNMPRF
                     MASGDTSRLTLDITNLTDKPQKLNVALTASGLLELVSDSPAAVELAPGVRTTLFIPVR
                     ALPGYGDGEIQATISGLALPGETVADQHKQWKIGVRPAFPAQTVNYGTALQPGETWAI
                     PADGLQNFSPVTLEGQLLLSGKPPLNIARYIKELKAYPYGCLEQTASGLFPSLYTNAA
                     QLQALGIKGDSDEKRRASVDIGISRLLQMQRDNGGFALWDKNGDEEYWLTAYVMDFLV
                     RAGEQGYSVPTDAINRGNERLLRYLQDPGMMSIPYADNLKASKFAVQSYAALVLARQQ
                     KAPLGALREIWEHRADAASGLPLLQLGIALKTMGDATRGEEAIALALKTPRNSDERIW
                     LGDYGSPLRDNALMLSLLEENKLLPDEQYTLLNTLSQQAFGERWLSTQESNALFLAAR
                     TIQDLPGKWQAQTSFSAEPLTGEKAQNSNLNSDQLVTLQVSNSGDQPLWLRMDASGYP
                     QSAPLPANNVLQIERHILGTDGKSKSLDSLRSGDLVLVWLQVKASNSVPDALVVDLLP
                     VGLELENQNLANGSASLEQSGGEVQNLLNQMQQASIKHIEFRDDRFVAAVAVDEYQPV
                     TLVYLARAVTPGTYQVPQPMVESMYVPQWRATGAAEDLLIVRP"
     gene            22762..23607
                     /gene="sseA"
                     /locus_tag="JNC24_000742"
     CDS             22762..23607
                     /gene="sseA"
                     /locus_tag="JNC24_000742"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HAY9157571.1"
                     /translation="MSTTWFVGADWLAEHIDDPEIQIIDARMASPGQEDRNVAQEYLN
                     GHIPGAVFFDIEALSDHTSPLPHMLPRPETFAVAMRELGVNQDKHLIVYDEGNLFSAP
                     RAWWMLRTFGVEKVSILGGGLAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDV
                     LLASHENTAQIIDARPAARFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDE
                     LDAIFFGRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLP
                     VEPVK"
     gene            complement(24100..24876)
                     /gene="sseB"
                     /locus_tag="JNC24_000743"
     CDS             complement(24100..24876)
                     /gene="sseB"
                     /locus_tag="JNC24_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HAY9157572.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIVPFYERRWGGFLRDFKQNRII"
     gene            complement(25018..26301)
                     /gene="pepB"
                     /locus_tag="JNC24_000744"
     CDS             complement(25018..26301)
                     /gene="pepB"
                     /locus_tag="JNC24_000744"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HAY9157573.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKAPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(26479..26679)
                     /gene="iscX"
                     /locus_tag="JNC24_000745"
     CDS             complement(26479..26679)
                     /gene="iscX"
                     /locus_tag="JNC24_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HAY9157574.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(26691..27026)
                     /gene="fdx"
                     /locus_tag="JNC24_000746"
     CDS             complement(26691..27026)
                     /gene="fdx"
                     /locus_tag="JNC24_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HAY9157575.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(27028..28878)
                     /gene="hscA"
                     /locus_tag="JNC24_000747"
     CDS             complement(27028..28878)
                     /gene="hscA"
                     /locus_tag="JNC24_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HAY9157576.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQAGIPDRSDNRVQRELLDAAIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDDAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(28895..29410)
                     /gene="hscB"
                     /locus_tag="JNC24_000748"
     CDS             complement(28895..29410)
                     /gene="hscB"
                     /locus_tag="JNC24_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HAY9157577.1"
                     /translation="MDYFTFFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSQAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNEAWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(29506..29829)
                     /gene="iscA"
                     /locus_tag="JNC24_000749"
     CDS             complement(29506..29829)
                     /gene="iscA"
                     /locus_tag="JNC24_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HAY9157578.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(29846..30232)
                     /gene="iscU"
                     /locus_tag="JNC24_000750"
     CDS             complement(29846..30232)
                     /gene="iscU"
                     /locus_tag="JNC24_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HAY9157579.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(30260..31474)
                     /gene="iscS"
                     /locus_tag="JNC24_000751"
     CDS             complement(30260..31474)
                     /gene="iscS"
                     /locus_tag="JNC24_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311423.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAY9157580.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(31715..32203)
                     /gene="iscR"
                     /locus_tag="JNC24_000752"
     CDS             complement(31715..32203)
                     /gene="iscR"
                     /locus_tag="JNC24_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HAY9157581.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRIQDAIDVKL
                     RA"
     gene            complement(32504..33241)
                     /gene="trmJ"
                     /locus_tag="JNC24_000753"
     CDS             complement(32504..33241)
                     /gene="trmJ"
                     /locus_tag="JNC24_000753"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HAY9157582.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKA"
     gene            33360..34163
                     /gene="suhB"
                     /locus_tag="JNC24_000754"
     CDS             33360..34163
                     /gene="suhB"
                     /locus_tag="JNC24_000754"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HAY9157583.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYSQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAMLANMRDELSDALKR"
     gene            34308..35162
                     /locus_tag="JNC24_000755"
     CDS             34308..35162
                     /locus_tag="JNC24_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAY9157584.1"
                     /translation="MALPVNKRVLKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILAVIGQDDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKETKRLILIPDGEHIDAFSDRHGDVYREQMVDFI
                     LSALNPQN"
     gene            35353..36633
                     /gene="csiE"
                     /locus_tag="JNC24_000756"
     CDS             35353..36633
                     /gene="csiE"
                     /locus_tag="JNC24_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HAY9157585.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATMATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFETRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQPVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYTHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(36625..37764)
                     /locus_tag="JNC24_000757"
     CDS             complement(36625..37764)
                     /locus_tag="JNC24_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HAY9157586.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLTLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     ALLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAVRQGSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(37924..38814)
                     /gene="hcaR"
                     /locus_tag="JNC24_000758"
                     /pseudo
     CDS             complement(37924..38814)
                     /gene="hcaR"
                     /locus_tag="JNC24_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311430.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
     gene            38950..40311
                     /locus_tag="JNC24_000759"
     CDS             38950..40311
                     /locus_tag="JNC24_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit alpha"
                     /protein_id="HAY9157587.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLRDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            40308..40826
                     /locus_tag="JNC24_000760"
     CDS             40308..40826
                     /locus_tag="JNC24_000760"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HAY9157588.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLEQLDKEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QISETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            40826..41146
                     /locus_tag="JNC24_000761"
     CDS             40826..41146
                     /locus_tag="JNC24_000761"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HAY9157589.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            41143..41955
                     /locus_tag="JNC24_000762"
     CDS             41143..41955
                     /locus_tag="JNC24_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HAY9157590.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAARYPGGGGPLYTASK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            41965..43167
                     /locus_tag="JNC24_000763"
     CDS             41965..43167
                     /locus_tag="JNC24_000763"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HAY9157591.1"
                     /translation="MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDERHLPYER
                     PPLSKSMLLEDSPQLQQVLPANWWQENNVHLHSGVTIKTLGRDTRELVLTNGESWHWD
                     QLFIAIGAAARPLPLLDALGERCFTLRHAGDAARLREVLQPERSVVIVGAGTIGLELA
                     ASATQRRCKVTVIELAATVMGRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVVDGEKV
                     ELTLQSGETLQADVVIYGIGISANEQLAREANLDTANGIVIDEACRTCDPAIFAGGDV
                     AITRLDNGALHRCESWENANNQAQIAAAAMLGLPLPLLPPPWFWSDQYSDNLQFIGDM
                     RGDDWLCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRPIRKWIQSGKTFDAKLLID
                     ENIALKSL"
     gene            43186..>43275
                     /locus_tag="JNC24_000764"
     CDS             43186..>43275
                     /locus_tag="JNC24_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HAY9157592.1"
                     /translation="MSTPGAWRGARVCITFKSHYLAKGLVMNTL"
BASE COUNT        10266 a        11949 c        10859 g        10201 t
ORIGIN      
        1 tttcctcttt tgatgatctt aaatgtctta tttttcgtaa tgtgtataac aaggaatagt
       61 gatgaaattt aaaaaatgtc ttctgcctgt ggcaatgtta gcgtcattca ctctggcagg
      121 atgccagtca aatgctgacg atcatgccgc cgatgtttat caaaccgatc aactgaatac
      181 caaacaagaa actaaaaccg ttaatattat ttccattctt cccgcaaaag ttgccgtaga
      241 caactcccaa aataaacgga acgcacaagc cttcggcgcg cttattggcg cagtcgctgg
      301 cggtgttatc ggccacaacg tcggttctgg cagcaattcc ggaacgacgg caggggcagt
      361 tggcggcgga gctgtaggcg cggcagcggg ttctatggtg aatgataaaa ccttagtgga
      421 aggtgtttct ttaacatata aggaaggcac caaagtgtat acctccaccc aggtgggtaa
      481 agagtgccag tttacgacag gtttagccgt tgttattacc acaacgtata acgaaacgcg
      541 tattcagcca aatactaaat gtcctgaaaa gagctaataa tcaggaggag tcatgaagaa
      601 agtttttctt tgcgccatct tagcctcctt aagctatccg gctatcgcct catcattgca
      661 ggatcaactc tctgctgtcg cagaagcgga acagcaaggt aaaaatgaag agcaaaggca
      721 gcatgacgaa tgggtcgcgg agcgcaacag ggaaatccag caagagaagc aacgtcgcgc
      781 aaatgcccag gccgccgcta acaaaagagc ggcaacggca gcggcaaata agaaagctcg
      841 tcaggataaa ctggacgccg aagccactgc ggacaaaaaa cgcgatcaaa gttatgaaga
      901 tgagctacgc agcttagaga ttcagaaaca aaaactgacg ctggcgaaag aagaagcccg
      961 cgttaagcga gaaaacgaat ttatcgatca ggaactgaag cacaaagctg cgcaaaccga
     1021 tgtggtgcaa tctgaagctg acgccaacag aaatatgact gaaggcggtc gcgatctgat
     1081 gaaaagcgtg ggcaaagcag aagagaacaa atcggacagc tggtttaatt aatcgatgtt
     1141 agtaacttca agcctatgat tcttgaagat aaaaaaccct ctgtagtaac agagggtttt
     1201 gttcattcat agtgcaggga ttaaaatcat tcccactcaa tggtagctgg cggcttgccg
     1261 ctgatgtcat acaccacgcg ggaaataccg ttcacttcat tgataatgcg gttggaaacg
     1321 cgaccgagga aatcgtacgg cagacgcgcc cagtgtgcgg tcataaagtc gatggtttcg
     1381 acagcacgca gagagacaac ccagtcatac ttacgaccat cgcccattac gccaacggaa
     1441 cgtaccggca ggaacacagt gaacgcctgg ctgactttgt cgtacaggtc cgctttacgc
     1501 agttcttcaa tgaagatggc gtcagcacgg cgcagcaggt cacagtactc tttcttcact
     1561 tcacccagca cacgaacgcc aaggcctggt cccgggaatg ggtgacggta caacatgtcg
     1621 tacggcaggc ccagctccag accaatctta cgcacttcgt ctttgaacag ctctttcagc
     1681 ggttcaacca ggcccatctt catctctttc ggcaggccgc ccacgttgtg gtgagatttg
     1741 atgacgtgtg ctttaccggt tgcagacgcc gcagattcga taacgtcagg gtagatggtg
     1801 ccctgcgcca gccacttcac gtcttccagt ttcagcgctt cttcatcgaa tacttcaacg
     1861 aaaacgcgac cgatgatttt acgttttgct tccggatcgt tttcgccagc cagcgctgac
     1921 aggaagcgat cttctgccgg aacgtgaaca atgttcagac caaagtgatc gccaaacata
     1981 tccagaacct gctctgcttc gttgagacgc agcaggccgt tgtcgacgaa tacgcaagtc
     2041 aggtttttac cgatagcgcg gtgcagtagc attgcggtta cggaggaatc cacgccacca
     2101 gagaggccga ggatgacttt atcgtcgcct acctgctcac ggatgcgggc tacagcatcg
     2161 tcgataattt tcgctggcgt ccacagggct tcacactggc agatatcacg cacaaaacgc
     2221 tccagcatgc gcataccctg gcgggtgtga gtcacttccg ggtggaactg tacgccatag
     2281 aagcgttttt cttcgttagc cataatggca aacgggcagc tttcggtgct ggctacggtg
     2341 acgaagtcgg acgggatagc ggtaacttta tcgccgtggc tcatccagac atcgagcagc
     2401 ggtttaccgt ctgcggtcag cgcatcttcg ataccgcgaa ccagtgcact gtcgtttacg
     2461 acttcaacct gcgcgtagcc aaattcacgt tcgttagagg cttcaacgtg accgcccaac
     2521 tgcattgcca tggtctgcat gccatagcaa acgccgaata ccggtacgcc tgcttcaaag
     2581 acatactgcg gcgcacgcgg gctgttttct tcagtagtac tttccgggcc gccggaaaga
     2641 ataatgccgc ttggattgaa gtcacgaatt tgtgcttctg tcacatccca cgcccacagt
     2701 tcgcagtaaa cacccagctc acgcacgcgg cgcgcaacca gttgagtgta ctgagaaccg
     2761 aagtctagaa tgaggatgcg atgcttatga atgttttccg tcattgacgc ttattccgag
     2821 gcaagtgaaa cagataatat aaatcgcccg acatgaagtc gggcgaagag aatcaggagc
     2881 ccagacggta gttcggggac tctttagtaa tggtcacgtc gtgaacgtgg ctttcctgaa
     2941 tgcccgcacc gctgatacgt acaaactccg ctttagtacg cagttcgtcg atagtaccac
     3001 agccggtcag acccatacag gagcgcaggc cgcccatctg ctggtgaatg atctctttca
     3061 ggcgaccttt ataagctacg cgaccttcga taccttccgg caccagtttg tcggcagcgt
     3121 tatcgctctg gaaataacgg tcagaggaac ctttggacat cgcgcccagg gaacccatac
     3181 cacggtaaga tttgtaagaa cggccctggt agagttcgat ttcacccgga gattcttcag
     3241 tacccgccag catggaacct accatcaccg cgcttgcgcc agcggcgata gctttggcga
     3301 tgtcgccgga gaagcgaata ccaccatctg cgataaccgg aataccggtg ccttccaggg
     3361 cttcaactgc gtcagcaacg gcagtgatct gcggaacacc tacgccagta acgatacgag
     3421 tcgtacagat agaaccaggg ccgataccga ctttaaccgc gctgcaacca gcttctgcca
     3481 aagcgcgtgc acctgcagct gttgccacgt tgccgccgat gatttgcaga tccggatatt
     3541 tagcacgggt ttcacggata cgctgcaaaa cgccttctga gtgaccgtgg gaggagtcga
     3601 tcagcagaac gtcaacgcca gcggcaacca gcgcgtcaac acgctcttcg ttacccgcac
     3661 ctgcgccaac tgcagcacca acacgcagac ggccttgctc gtctttacag gcgttcggtt
     3721 tacgttccgc tttctggaag tctttcacgg tgatcatgcc gatcaggtgg aattcgtcat
     3781 caaccaccag cgctttttca acgcgttttt cgtgcatttt tgccagcacc acttcacggg
     3841 cttcaccttc acgcacggtg accagacgct ctttcggcgt catgtaaacg ctaaccggct
     3901 ggttcaggtc ggtaacaaaa cgcacgtcac gaccggtgat aatacccacc agttcgtttt
     3961 cttcggtaac gaccggatag cccgcaaaac cgttacgctc ggtcagttct ttcacttcgc
     4021 gcagcgtcgt ggttggcaga acagtctgcg gatcagtcac cacaccagat tcgtgttttt
     4081 tcacacggcg aacttcttct gcctggcgtt caatggacat gtttttgtgg ataaagccga
     4141 taccgccttc ctgagccaga gcaatagcca ggcgcgcttc cgttacggta tccattgctg
     4201 cggaaagcat agggatattc agacgaatag ttttcgtcag ctgggtgctg aggtcagcag
     4261 tattcggcag aacggtagaa tgagcaggaa cgaggagaac gtcgtcaaac gtcagagctt
     4321 ctttagcgat acgtagcatg ggcaatatct cgaccagagt ggttaataaa tattgccgcg
     4381 gcattataca gagcgtaacc gattgcatct accccttttt gcaaaaaatg cttgctatcc
     4441 ccgaagggcg ggttactatc gactgaataa cctgctgatt tagaatttga tctcgctcac
     4501 atgttacctt ctcaatcccc tgcaattttt accgttagtc gcctgaatca aacggttcgt
     4561 ctgctgcttg agcatgagat gggacaggtt tggatcagcg gcgaaatctc taatttcaca
     4621 caaccggctt ccggtcactg gtactttaca ctcaaagacg acaccgccca ggtacgctgc
     4681 gcgatgttcc gcaacagcaa ccgccgggtg accttccgtc cacagcatgg acaacaagtt
     4741 ttagttcgcg ccaatattac gctctacgag ccgcgcggtg actaccagat aatcgttgag
     4801 agtatgcagc cggccggtga agggctgctg caacagaagt acgaacagct caaagcgaag
     4861 ttgcaggctg aaggtttgtt cgatcagcaa tacaaaaaac cacttccctc ccctgcgcat
     4921 tgcgttggtg tgatcacctc aaaaaccggt gctgcgctac atgatatttt gcatgtgtta
     4981 aaacgtcgcg atccttctct accggtgatc atctacccca ccgccgttca gggcgatgac
     5041 gcaccggggc aaattgttcg cgccattgaa ctggcgaatc agcgcaatga gtgcgacgtg
     5101 ttgatcgttg ggcgcggcgg cggttcgctg gaagatttat ggagttttaa cgacgaacgc
     5161 gtagcgcggg cgatttttgc cagccgcatt ccggtcgtca gcgccgtcgg gcatgagacg
     5221 gatgtgacca ttgccgattt tgttgccgat ctgcgtgcgc caacgccgtc tgccgccgct
     5281 gaagtagtga gccgtaatca gcaagagtta ctgcgccagg tgcaatcgac ccgtcaacgg
     5341 ctggagatgg cgatggatta ttatctcgcc aaccgcacgc gtcgctttac gcagatccat
     5401 caccgattac agcaacagca tccgcagctc cggctggcac gccagcaaac catgcttgag
     5461 cgcctgcaaa agcggatgag ctttgcgctg gaaaatcaac ttaagcgtac cgggcaacag
     5521 cagcagcggt taacacagcg gctgaatcag caaaatccac agccgaagat tcatcgcgcg
     5581 caaacgcgca ttcagcaact ggaatatcgt ttagcagaaa ccctgcgcgt acagcttagc
     5641 gccacgcgtg aacgtttcgg taatgcagta acgcacctcg aagccgtaag cccactgtca
     5701 acgctggcgc gtggatacag cgttactact gctactgacg gcaatgtact gaaaaaagtg
     5761 aagcaagtta aagcgggtga aatgctaacc acacgtctgg aagacggctg gatagaaagt
     5821 gaagttaaaa acatccagcc agtaaaaaaa tcgcgtaaaa aggtgcatta agccagcacg
     5881 aattcaacgc gttttttcga tatcaagcca tgcccgtgct gacagaagta atctactgca
     5941 ccgcaggctt ttaacacctg caacggttga tggcagtctg gacaaagagc tttcatttca
     6001 ataatttccc cgcaggatgg gcaacgcgca tggccgttat cctgatcaag cacatgctga
     6061 cattgtggac aatgcagttc catatgaatt cctccggtag ccatccctct acattcatag
     6121 agggaatggc agataaaata cttacggata attatttatt tttcttgatg tgcttcatca
     6181 gacgcttacg tttacgcatc tgggttggcg tcagggtgtt acgcttgttc gcatacgggt
     6241 tttccccttc tttgaactga atacgaatcg gcgatcccat tacgtccagc gatttgcgga
     6301 agtagttcat caagtagcgc ttgtaggaat caggcaggtc tttcacctga ttaccgtgaa
     6361 tcaccacaat cggcgggtta taaccaccag cgtgggcata tttcagcttc acacgacgac
     6421 cgcgtaccag cggcggttgg tgatcttcaa cagccatcgt catgatgcgc gtcagcatag
     6481 aggtccccac acgacgggtg gagctgtcat acgcttcacg tactgattca aacaagttac
     6541 caacaccact gccgtgcaag gcagagataa agtgcacacg agcaaaatcg ataaagccca
     6601 gacggaagtc cagcgtttct ttcacctgct ctttcacttc ctggctcagg ccatcccact
     6661 tattcaccac aatgacaagt gagcgcccac tattgagaat aaagcccagc agcgagagat
     6721 cctgatcgga aataccttcg cgcgcatcaa tcactaacat caccacgttg gtatcttcaa
     6781 ttgcctgcaa cgttttgatt acggagaatt tctctacagc atcggtgatt ttgccgcgtt
     6841 tacgtacgcc agcggtgtca atgagcacat actcacgtcc atcgcgttcc attggaatgt
     6901 agatactgtc acgcgtcgtg ccaggcatgt cataaacaac aacgcgctct tcaccaagaa
     6961 tacggttagt gagtgtagac ttacctacgt tcggacgacc cacaatcgcc agtttgatcg
     7021 gcagactttg cgggtcgaag tcgtcttcct cttcttcttc gccgttctct tccgcttcaa
     7081 attgcgccca gtattcagcg tcttcgtcga cttcctcttg cggtgcgaga tcttccatcc
     7141 acggcagcag cacacgctcc agcagactta atacgccacg accgtgagat gcggcgatcg
     7201 ggtagatttc acctaaacca agcgagtaga aatcaaccac tgcctgatcg ggatccagac
     7261 cgtcagtttt gtttgccacc aggaaggtcg gtttttcacg agagcgcaga tgtttggcaa
     7321 tcgcttcatc tgccggcatc aagcccgcgc gcgcatccac cataaacagt acgacgtccg
     7381 cttcttcaat cgccagcagc gactgttccg ccatgcgggt ttctacaccg tcttctgtgc
     7441 catcaatccc gccggtatca atacagataa actcacggcc ttcaatttcc gcacgaccgt
     7501 acttacggtc acgagtcaga cccgggaaat ccgcaaccag cgcatctcgg gtgcgagtta
     7561 gacggttaaa taacgtggat tttcctacgt tagggcgccc gacaagcgcg accacaggta
     7621 ccatgtttaa agcctcattt ttataaatca tcagacaacg cacgctatat tcgcgtcgtt
     7681 gttaaaaaca ggaaaacggc ccctgtccag gagccgtttt caaagtgaac gacagagacg
     7741 attaacgtgt aatagagtac acggttccgt cttttgcctg gatcagcagt ttgccgtcag
     7801 cggcaaccgg ttcagtctgg aaaccggaac tatcaacttt ttgctgggca acgaaacgac
     7861 catcttcgac gttaatccag tgcagataac cttcactgtc acctaccacc aggttgccat
     7921 tatacagcac cggcgaagtc agcaggcgat gcagcagatc gctttgtgtc cacagcgtaa
     7981 cgccgccatc aatggtcaac gccatcaccc ggtcattttg atcgaccaga tagatgcgat
     8041 tgccgtcgac gatgaaatca ttcaccgaac ccagttcgcg tttccacata atctgaccac
     8101 tgcgcagatc aagcgccgtc aggttaccat tataggccag agcgaaaaca acgccgttaa
     8161 caacgacggg agtcgtgtca acatcgctca gacggtcaat ttcggtagaa ccggtcgcct
     8221 gggaaatacg ctgctgccaa atcatctggc cctgttccat cagcactgcg ctgacgcgac
     8281 cattatcgcc ccccacgacg gccgcaccaa aagccgttgc cggcgcagac tcgccacgca
     8341 aagagagcga aggcatatcg aggttaactg tccatttgac agcgccgtca gcttcgttca
     8401 gcgcttgtaa ctgaccgtta ctagtgtgga ttaacaccag accgtcgctg accaccgggc
     8461 gagaaagtgc ttcacccgcg actttagttt gccatgccac agtaccatcg ctggtattca
     8521 gcgcgtaaac ctgcgccttt tcgctgccaa tgtagacatg cccaccagac acggtcacac
     8581 cgccagaaag taatgcaggc tctttagaga accagccatc tttctcggcc aggctgacag
     8641 accagatttc tttgccatca tccgcattca gcgcttttac caaaccagcg cggtccgctg
     8701 catagacaac gttgtccgcc agtgccggat gaagattgga atagaagttg ccaatgccgc
     8761 taccaacgga agtgctccac gccgtggtcg gcgtaaactg gttttcaacg gttggcaatg
     8821 gggacatctt taccacatct tcttcgctgt taaacagcga acagccgctt aaaagggtaa
     8881 cggaaagcag tcctggcagc agtaatttac gcaattgcat cgggtccctc tcagatggac
     8941 aaattattaa ttttcatctg catcatttcg ctcagtgccg gagtaacatc gcttttcacg
     9001 cctgcttccc atgcactacg cgcaccttgc ttatcacctt tgctcaacaa tgctttacca
     9061 cgcaggtcgg caacaatggc agcccaccct tcacctttga tggtatcaag ggttttcagc
     9121 gcggcatcag cctgcttgag ctgtacctga acgcgagcaa gacgcagatt tatcacggct
     9181 ttgagatttt catcactcgt gtctgccagc ccctgttgta actgggcggc agctttctct
     9241 agttcatttt tgtcaacaaa ttgctgcgca agttccaaag aagccagcgc accataagta
     9301 tttttatttt cagcagcaaa tttttccgcc gccgggatgc tatccggttt gccttcgctc
     9361 actgcggtaa ccgcattttg ataggcaaga gaagcggagc gtgcagaatc aacctgatgg
     9421 ctgttccagt agcgccagcc aatcagtgcg ccaacgccca aaatcacccc aacagccagt
     9481 gctttgccat tttcagcaaa aaagcgttta accgcttcta cctggtcgtt ttcgttctcg
     9541 taaatttcca cgctgtcctt ctccttcctt aacccagtaa cgtgcgcaaa tgcgcggcta
     9601 cgctatcctg cgcaactgcc gtttgctcac cagagcgcaa atccttcact actgctgtgc
     9661 cgttagccac ttcagactca cccagcacca cagcaacgcg ggcaccccat ttatcagcac
     9721 gggcaaactg tttcttaaag ttgccgccgc cgtggttggt catcaatttc acgcccggta
     9781 attcatcacg cagacgctca gctaatgcca tagccgcaga ttgtgtatca gcacctgaag
     9841 ccaccaggta tatatcgaca acaggatcgg ctttaaattc cggattaacg gcctgtacta
     9901 acaatacaag acgttcgagg cccatcgcaa aaccgactgc cggtgttgca cgaccgccca
     9961 gttgttccac cagaccgtca taacgaccgc ctgcacacac ggtgccctgg gagccgaggc
    10021 tgttagtcac ccactcgaaa acggtacggt tatagtaatc cagaccacgc accagacgct
    10081 ggtttacggt gtaagcgatc cccgcgctct caagcagttt gcacagaccg gcaaaatgct
    10141 cacgagattc ctcgtccaga taatcaccta atgccggagc gtcgttgaga agcgcctgca
    10201 cttccggatt ttttgaatcc agcacgcgca gcgggttagt gtacatgcgg cgtttgcagt
    10261 cttcgtccag cttttcttta tgctgctcaa ggaatgccac cagcgcatcg cggtaattgg
    10321 cgcgtgcttc cagcgaacca atagagttca gctcaagagt tacgtgttcg aagataccca
    10381 gcgcacgcca ccagcgggca gtgagcataa tcagttcagc gtcgatatcc ggaccttgca
    10441 gaccgaaaac ttcgcagccc aactgatgga actgacgata acgccctttc tgcggacgct
    10501 cgtgacggaa catcggcccg atataccaca gacgctgttc ctgattgtac agaagaccat
    10561 gctcgatgcc ggcgcgtaca cagcccgccg tcccttcagg gcgcagagtc aggctgtcgc
    10621 cattgcgatc ctcaaaggtg tacatctctt tttcaaccac gtcggtgact tcaccaatcg
    10681 cacgtttgaa tagcggggtc tgctctacaa tcggcaagcg gatttcactg taaccgtagc
    10741 tgccgagcac gtttttcagt gtgccttcaa tgcgctgcca gatggccgtt tcgccaggca
    10801 ggtaatcgtt catgccgcga atggcttgaa tgttttttgc cacgtttatt ctctttctga
    10861 atataaaaat gaaccctcaa cgcttccctc aatgtttcgg gagccatgcg ggttcaatca
    10921 tacacgggaa gcgaggcgct tcccatcacg ttattatttt tcaacctgct gaacgtcaat
    10981 tcgacgcgct tcgtccagct gactggcttt cgcacgaatg cgtgcttcca gctggtcgat
    11041 catatcgttg ttgtccagac ggtctttgcg cacgccatct tcatagaggc cgcttttctt
    11101 gttgccgccg gtgacgccga gtgtagaaac cagcgcctca cctgggccat tcaccacgca
    11161 gccgataatc gaaacgtcca tcggagtgat gatatcttcc aggcgttgct ccagcgcgtt
    11221 aaccgtaccg ataacatcaa attcctgacg cgaacaggtc gggcaggcga tgaagttaat
    11281 ccctcgcgaa cggatacgca gcgatttcaa aatatcgaaa ccgactttga tctcttcgac
    11341 cggatcggcc gccagcgata cgcgcagcgt gtcgccgatg ccttccgaca gcagcagacc
    11401 taaaccaatg gcggatttta ctgccccgct gcgtgcacca ccggcttcgg tgatccccag
    11461 atgcagcggc tgatcgatct gttttgccag caaacgataa gactcaacag cgaggaagac
    11521 gtcagacgct ttcacgctga ctttgaactg atcgaagttc aggcgatcga gatgatcaac
    11581 atgacgcatg gcggattcca gcaacgcctg cggcgtcggt tcgccatact tttcttgcag
    11641 atctttttcc agcgatccgg cgttaacgcc aatacggatc ggaatgtttt tatcgcgcgc
    11701 acagtcaacc accatgcgaa tacgctcttc attaccgata ttgccagggt taatacgcag
    11761 acaatcgacg ccgtattccg ctactttcag cgcaatgcga tagtcgaagt ggatgtcagc
    11821 caccagcggc acgttaaccc gctgtttgat gagtttgaac gcttctgccg cgtccatcgt
    11881 cggtacggag acacggacga tatcagcgcc aacgcgttcc agcgccttga tttgattgac
    11941 cgttgcttcg acatctgtcg tacgcgtatt ggtcatggac tgtacggcga tgggagcacc
    12001 atcgccaatc ggcacattcc caacgtaaat acgtgttgat tttctacgtt gaattggagc
    12061 ctggttatgc atgaaaaatc tcccgcgtta cccgtctgtt actgcgccgg tgattgttcg
    12121 gcattgaggg tcagacgcgc aacctggtta gttctgataa aacgactcag atcgacaggt
    12181 ttcccttgat actggatctg tactgcggct ggcgcaccaa ttttcagttt atacggtgcc
    12241 tggccggtta aattcaaatt accgtcttta cgctgcatcc cgctaaacaa ttttttaccg
    12301 gtagcatcag tgacctccag ccagcaatcg gcagtaaagt tcatcaccag cgcattcgga
    12361 tcagccgccg gcgtggtcac gccagcctga tcggttggca acggcgcagc accatctggc
    12421 gttgttgctg ccgttggtac cggggtcgcg gcggtatcaa catttgcctg cgaaggcgaa
    12481 acaaccgcat tctgttgcgg atcaacagct ggtgctggcg cagttacggc aggtgtttgc
    12541 gtgttggttg cggtagtatc cacagacgct ggcggcgtgg acgttgtagc cgggtctgta
    12601 gttgtcgacg tatttaacgg gacactctgc ccctgctcgc tattactgct cagttctgcc
    12661 gaagattgat cggccatagt ggtgatctct tcctgctgag ctttgtggtc ttgccaccac
    12721 caggcaccgc tcaggccgat aaccacaaac aacaccagcc aggtgaaggt catcagccag
    12781 ccatcacgtt ttttgcggcg tttaccgagg gaaaaactct gcattggcgc aacttttgca
    12841 gcccgtagtg gagcctgctt ttccagccct ggcagcagtt cttcttctgg aatatgtacc
    12901 agacgcgcat aagagcggat atatccgcgc aggaatgttg aagcaagatc ggcgggtgcc
    12961 ttatcttctt caatgtcgcg taccgtggaa accttcaggc aaagtcgctc ggcaacggcc
    13021 tgctgactaa gtcctagttg ttcgcgagca ttacgcaggc gagcgccggt agtaagtgct
    13081 tcattttggt cgtgcgtggc ttcagtattc attcgctgca ggtacgttta aatgagaatt
    13141 gagatgccgg tgaaccatca tgctcaccca caccgcgaaa catccgttaa gttaaccgtt
    13201 atcatacagt ataagaccgt ctgtgtgctc gtgacaaagc ccacattcac taaggctaac
    13261 ttactgttgc atcgttacat actgccttaa agtcagcaaa aacgcaccgt taatattgac
    13321 cagacaattg caacttaata cttcattcat aaaacattac gccacggtac ataaagtaac
    13381 cgtggcgtaa tggctatcag accgctttaa tgtcgatggc ttcaccctgc atccgtttac
    13441 gcagggtacg tttcgtacgg tcgataacat cgcccgccaa ctgaccacag gcagcatcga
    13501 tatcatcacc acgagtttta cgcacaatag tggtgaaacc gtagctcatc agcacttttg
    13561 agaaacggtc gatacggctg ttcgagctgc gtccatacgg cgcacccggg aacgggttcc
    13621 acgggatcag gttgatctta cacggcgtat ctttcagcag ttccgccagt tggtgcgcgt
    13681 gttcagtgcc gtcgttaacg tggtcaagca tcacgtattc aatagtgact cggccctgat
    13741 tggcgttgga tttctccaga taacggcgca ccgcagcaag gaacgtttcg atattgtact
    13801 ttttgttgat cggcacaatt tcgtcacgaa tttcgtcgtt cggcgcgtgc agggaaattg
    13861 ccagtgcaac gtcgatcata tcgcccagtt tatccagcgc cggaactaca ccggaagtag
    13921 aaagcgtgac gcgacgttta gacaggccaa aaccgaaatc atcaagcatg atttccatcg
    13981 ccggaacgac gttgttcagg ttgagcagcg gctcgcccat gcccatcatc actacgttag
    14041 tgattggccg ctgaccggtg acttttgctg cgccgacgat tttcgccgcc cgccacacct
    14101 ggccgataat ttccgacacc cgcaggttgc ggttaaagcc ctgctgggcg gtggaacaga
    14161 atttacactc cagcgcacac cccacctgcg aagagacgca gagcgtggca cggtcgtctt
    14221 ccgggatata caccgtttcg acgcgctgat cgccaacggc gatcgcccat ttaatggtgc
    14281 cgtcggatga acgctgttct tcaaccactt ccggtgcgcg gatctccgcc acctctttca
    14341 gtttgccgcg caacactttg ttgatgtcgg tcatctcatc aaagttgtcg cagcaatagt
    14401 gatacatcca cttcatcacc tgatcggcgc ggaagggttt ttcacctaaa tctttaaaaa
    14461 actcccgcat ctgctgacgg ttgagatcca gcaggttgat ttttccatct ttcgtggtga
    14521 cgttttcagg tgtgactaat tgttcagaca tatgctattc cggcctcgtt attacacgtt
    14581 atggcccctg gagggttgaa aaaagaaacg ccccggtgag cggctgctcg tccgggggcg
    14641 ctgcattgta caaattctgg cgcacggatg ccacgtttgc acgcggcatt tacgaaatta
    14701 ttaacgggtg cgcgggcaca cttcgccttc gccgaagaaa taagcgattt cgcgagcggc
    14761 agattcgacg gaatcagaac cgtgggtacc gttttcggtc aggctgtcag cgtaatcagc
    14821 gcgcagagta ccggccagtg cgtttgccgg attggtcgcg cccagcagat cgcggtgacg
    14881 ctgaacggcg ttttcacctt ccagcacgga aaccacgatc gggccagagg tcatgaattc
    14941 aaccagacca tcaaagaacg gttttccatc gtgttcagca taaaagccac gagcctgttc
    15001 aacggtcagg tgcagcattt tggtgccgac aattttgaac cctgcagctt caaagcgcgc
    15061 aaagatatta ccaatgacgt tttttgctac cgcgttcggt ttgatgatgg aaaaagtacg
    15121 ttcaatagcc atttttacct ctgtaaattg ttctgttgtt gtctgtacca gcgtacgaga
    15181 tggcgcggat tataatgagc aacagggccg ttgactattg atgaaggtaa cattttttta
    15241 aaataaaatg agttttagca acaatcaggg acaaaacaga ctattgcatg acaaatttca
    15301 ctgtcgcgat ttgccccacg tcatccatca ccagcaattg ataatcacct ttatccgtca
    15361 aatgcagggt cacgttgcgc ccgcgttcag ttaacggttc gccattcaga aaccaccagc
    15421 gttcacctgc ccctccactt gattgcaacg gcaaagtcgc ttccgctgcg cccggtaaac
    15481 gtttaataat cgcgccatcg cgaacgcctg tcagctgcag agggagctgg gcatcgtgac
    15541 cgtatggcgg gcaaattgtc gaggccggtg gtaagcgcac agcgcgacgt tctgatgcgg
    15601 gcagccaggg ttccagcggt agcggccaga catttaccat ttcttgcttc gcctgcgggc
    15661 agtcagcggc gacgcgctgc cctttgtcat ccagccagat ggggaagcga atgccattaa
    15721 tcccttcctg ctctgacagt aacagcgttg gcggctggct gccatccagc agccaggttg
    15781 ccaggcggcg gcgacagtta ccgtcacctt ccggcaaaga ctgtccgccc ggccagcaga
    15841 taacgccacg agtgactgag ttcgggcgcg ggtcttccgg cagattcgca ctgcgcgaca
    15901 gtaagatatt attgacctga ttcaacaatg gtacggcact ggcaaagcca aactgaccaa
    15961 caacgggcgt gccgtcaggt ctgccagtcc agatcccaat gacatagcga gcgttaaccc
    16021 caatcgccca ggcgtcacga tagccatagc tggtgcccgt tttccatgcc agtggggcga
    16081 cgcgcggcaa ggcactatcc ggcaagggtt gcgcttcatc agccataatc cggcgaatga
    16141 tccacgccgc ccccgacgac attaaaggcc gttcaagcag cggatcgtca ggctgtaagc
    16201 gcaatttgcc tgccttgccg tggcgagcaa acgcggtata cgctgccgcc atatcttcca
    16261 gttttgcacc agcgccgccg agaatgagtg aaagattcgg cgcagcgccg ttgggcaaat
    16321 ataacggcaa tccaacattg cgtaactttg ccgcaaaccg tttcggtcca taggcttcca
    16381 gcacctgcac agcaggtaag ttcagcgagc gcaccagcgc ctcgctcatg ctgatcgggc
    16441 catgaaaacc gctatcaaag ttacctggtc gataatcacc ggtgcgccgg gggacgtctt
    16501 gcagcagtga tgccgggtgg atcaagcctt catccagcgc cagaccataa acaaacggtt
    16561 tgagcaccga tcctggcgat cggatcgcat tgaccatatc aacatgacca aagcgtgaat
    16621 catcgttgag atcaaccgat cccacccagc cgcgaacacg catatcggta tgatcaacca
    16681 cgatcatcgc cagtgagctg cgcggtggca accgcccttt ccagttttgc gccagttctt
    16741 ccagacgtcg ttgaagaccg gcatccagcg tagtaacgat tttgtcgctt ttgcttttac
    16801 cgagcatcat gcgcgaaaac agcggtgcca gttgcggcat ttgtcggggt gccagccaga
    16861 tgggttcttc ccttgactct tttacctgct cacgggacca cacaccttgc gcagccatcc
    16921 gttcgagcac tttattacgc gcggcttcgg cacgctccgg ccaacgatcc gggcgaagac
    16981 ggctgggcgc ttgcggcaaa accgccagca ttgccgcctc ggaatagctt aaattcgcag
    17041 gcgactttcc gagataagcc caacttgccg caccgatccc ctgcaacgta ccgccaaacg
    17101 gagcgcggtt aagatacaag gtcagaattt cacgcttaga cagatgccat tccagttgca
    17161 acgcgcgcca gagctggcga attttgccgc caaatgtttt ggggtgagga tcaagcagac
    17221 gagcaacctg catagtgagc gtgctgccac cggaaataac ccgtcccgaa gtgagatctt
    17281 gccatgctgc gcgcgccacc gagaatggat tcacccccgg atgcttccag aaccagcgat
    17341 cttcataatt gatcagcgct tcaaggtaac gtggagaaac atcttcgatt gttaccggat
    17401 aacgccagat gccgtcagca tcggcgaagc gccagagcgg cgtaccatcc tgcgccacga
    17461 ccactcgtgc gggattgact tcatgcagcg gtagaggcca gagtttatct gctccccacg
    17521 ctgcaagaaa aaggagaaat ggcgcagctg ccagcgttat ccagcagccg cgtttggtta
    17581 acaagcgagg catttacggt cggacaatca gcagatcttc agccgcgccg gtcgcccgcc
    17641 attggggaac atacattgat tccaccatcg gttgcggtac ctgatacgtt cccggcgtca
    17701 ccgcccgcgc cagatacacc agcgttaccg gttggtattc atcaacggca accgccgcca
    17761 caaagcgatc gtcacggaac tcaatgtgct taatactcgc ctgctgcatc tggttcagta
    17821 agttctgcac ttcgccacca ctttgctcca ggctggcgct accgttcgcc agattctggt
    17881 tttccagttc cagacccaca ggcagcagat ccacgactaa cgcatccggc acgctgttac
    17941 tggcttttac ctgcaaccac accagcacca gatcgccgct acgtaacgag tccagcgatt
    18001 tgctcttacc atcagtacca agaatatgac gctcgatttg cagcacattg ttcgccggta
    18061 aaggtgcgga ttgcggataa ccgctggcat ccatacgcaa ccataacggc tgatcgccac
    18121 tgttgctcac ctgcaaggtg acaagttgat cgctattcag attgctgttt tgcgctttct
    18181 cgcctgtcag cggctcagct gagaaagagg tttgcgcctg ccatttaccc ggtaaatcct
    18241 gaatcgtacg ggcagccagg aacaacgcgt tactttcctg cgtcgatagc cagcgttcac
    18301 caaacgcctg ctgcgaaagt gtgttcagca aagtgtactg ctcatcgggt agcagtttat
    18361 tttcttccag caaggagagc attaacgcgt tgtcgcgcag tggactaccg taatcaccca
    18421 gccatatccg ctcatcacta tttctcggcg ttttcagcgc cagcgcaatc gcttcttcac
    18481 cacgcgtcgc atcacccatg gttttcagcg caatgccaag ttgcagcagc ggtaaaccag
    18541 aagcggcatc tgcacgatgc tcccagattt cacgcagcgc acccagtggt gctttttgct
    18601 gacgggccag caccagcgca gcgtaagact gaacggcgaa tttactggct ttgagattat
    18661 ccgcgtacgg gatcgacatc atgcccggat cttgtaaata gcgcagcaga cgctcattac
    18721 cccggttaat ggcgtctgtc ggcacgctgt aaccctgttc gcctgcgcgg accaggaaat
    18781 ccatcacgta agccgtcagc cagtactctt cgtcaccgtt tttatcccac agcgcaaagc
    18841 cgccgttatc acgttgcatt tgcagcaaac gggaaatgcc gatatcgacc gatgcacggc
    18901 gtttctcatc actgtcgcct ttgatgccca acgcctgcag ttgggctgcg ttggtataaa
    18961 gtgacgggaa caggccgctg gcggtttgct caagacagcc gtacggatac gcttttaact
    19021 ctttgatata acgtgcaatg ttcagcggtg gtttgccgct caacaacaat tgcccttcca
    19081 gcgtaacagg cgagaagttt tgcaatccat ccgccggaat cgcccatgtc tcaccaggct
    19141 gtaacgccgt accgtaatta accgtttgtg ccgggaacgc cggacggacg ccgattttcc
    19201 actgcttatg ctgatcggca acggtttcac ccggtaacgc taacccgcta atggtggcct
    19261 gaatttctcc atcgccataa cccggcaatg ctcgcaccgg gataaacagc gtagtacgca
    19321 cacctggcgc taattcaacg gctgcgggtg aatcgctgac cagttcaagc aaaccactgg
    19381 cggtcagggc aacgttcagt ttttgcggtt tatcggtaag attagtgata tccagcgtca
    19441 gacgcgaggt atcgccactc gccataaagc gcggcatgtt cagttcagca atcaccggtg
    19501 cggcaactat cactttgctt tcgttgctac cgaaatcatc tgccgtccag gcttgcgcca
    19561 tgacgcgcaa ttcaccgtta aaatcgccaa tcggcagtgt aaccgagcct tcgccctgtt
    19621 cgttgagcgt taccgacagc gcctgctgcg cgacaatatt gacgtgattg accggcggtt
    19681 taccaccacg tttcagctca tcaccatcgc caccgaaacg cagagctgcc agacgcccct
    19741 gaccttcaat aacctgaccg taaatatcgt aaatgtctgc gccatagcgt ttctgaccaa
    19801 agaacgcctg ccacggatct ggcgtaacgt agtcagtaat attcagcaca ccactatcaa
    19861 cggcagacac cagcacattc acctgtttag gcttctcgcc atttttagtg ctggctttaa
    19921 ttttcacggt taatggttga ttgggacgca tttttgctgg tgtttccagc gccagatcga
    19981 ggcgacggtt ttcatcacca agcggcagat gcaacacacc aaccgcgcgt tttggcgtcg
    20041 cggagcgaga tttatcgcca ggacgcacca ccagcgtact caaataaaga tcatgacgat
    20101 tccaggtttt atcgaccgga atcgtcagat ccagcccttg agccggaaca tcaatctctt
    20161 gccaccacag cggcccttca ctggactcga ccatcgcata acctttaccc gccgttggcg
    20221 cggcaatatg caacttaatg gtatcgccgg ggcgataact ggctttatct agtttcagcg
    20281 tgacacggtc gggtcgcact gcgccgctcc cgtcgctgtt gtcctgccag ctatagccag
    20341 cccaaaaacg cacgctactg accgcttcat tcggcgcttt gacttccaga cgataagcgc
    20401 cccactctac cgggaaactg accttaccgg tttcatccgc ctgaagatcc agagtttgtt
    20461 cattttcgat cagatctttt tgatcaaact gtgactgcca gccttcatct tctgaccagt
    20521 tccagtagta atcgcggcgt tcgcgaatca ggcgcacctg caagcccgac acggcttttt
    20581 tcacgccttg cgcatcgcta taaacgatgt caaaagcggc gttactgcct tcatcaacaa
    20641 tgggctgttt caccgtgctg tcagtgcgat aatcgtaaac cgatttcaag gcgaactgcg
    20701 gacggagccc cggcaatgca tcggcaggcc agatagcctg ctcagcgcgg cgcgtcaccg
    20761 ggcgaccgcc cgattccagc aaactgccct ggaaaataac ctgcaatggg gaatgcgttt
    20821 ccttccactg gctttctgta gaaacttcgc cgcgcccttt atcatccagc gtcaactgaa
    20881 cttcatccag cgtgcgggaa aggttttcgg cagctatatc gccgaattca aaaccaggta
    20941 aggctgacac agcctcacgc agtgggcgca ggaaaagttg cccttgcaaa gtattaccat
    21001 tagcaggcgc accgtacagg tagtatccca ccacggagaa tttcacttca tcattcggcg
    21061 taagcggggt tttctcaccg gtcagattca gcgccatacg ctctggcata aaatcttcga
    21121 cgtggaaatc ccacatccgg tactggttat cgcccgtgtt ggcgcgaata tgccacatac
    21181 cggttgccgc attgctatcg agtggccagg taaagtggta gaggccattc tccggctgac
    21241 taacgacgtt cctgagtacc tgcccgtcag gtttaatcac gtctaacttg atgggttgat
    21301 cgggcaatgc tttaccgtct gcatcgcgca gtaaaccatt gaggattacc gtttcacctg
    21361 ggcgataaag atcgcgtggg ccaaacatga aaaactgttt gctatagcct ggcgcgccag
    21421 caatgttaaa ttctgctaag tccagcgccg gaagttttaa atcgagtagc gttgtctgac
    21481 cgtctttacg tgccagcagt aatgccgcat ttttatcatt ttccagctgc acatgcccct
    21541 gagcgtcact ggttgcctga gtcagagtct gtcctttctc atttaataaa gagacttcaa
    21601 ttccttgctg ggccgcgccg ttttccagac tttgggtaaa gatatccaga cgattgtgat
    21661 aacggtgagc tgaaacgccg atatcactta acgtaaacag cgtcgcggga ttactgtaat
    21721 cgtaacgtcc agcctgattc atcacagcca gatacacgcc cgcctgctga agcggtttga
    21781 tatcgcccag cggcagcaat aatttttcac gggtgttacg cgcaggattg agatcaaacc
    21841 gtccggtgta gaccagatcc gccatctgca gcagtttgtc tgactgccag ttcgccagcg
    21901 aattgcggta ttcccattgg ctaatgaatg ctggcagaga ttctggctta acgcggaaga
    21961 agttaacatc gacattatta acgttgagcg ccattaccgg cagcccttca acgactttgc
    22021 caggcagcag cgaaccacgg ctggcaaaac cgacgctggg ttggatgtcg cgggtagtta
    22081 tagttttttc gtaatcttta ctgaaggttg cgttgttgag tgctttgacc tccttgccaa
    22141 tagtaacgat caaatcacgt ttcggttcga ggtggcgtaa acgtagctct ttaagattat
    22201 ctgacagctc ccaggcacca tccactttgc cgcttttttt atcgacgaca tgaataacgc
    22261 gtgagaaatc ctgatccggg tcgagaggga tggagaacgt cagcaccagc gtggctgcac
    22321 catcaagttg gacttcagag agatccagca atgtcagcac cttaccggca ctctgttggg
    22381 ccagtttttg tctctccggc acggagagtt ttgctgaact cgcgttttct gaagaggccg
    22441 ctttagtaac ttcagaaggc gcatcttttt tcaccgctgt tggcgcgtta tcgttgttgt
    22501 cgcaccctgc cagcgccagc attagcatgc aggcggctac gcgtaacttt ttcatttttc
    22561 atccctgcca caatggcccg ttagcaacgt cgaataatta ttatgcgtga gaatttacgt
    22621 tatgtaattc agtatcacgc ccatacaatc cgaattttag tgagaattgt ggctcagaca
    22681 gccaaatcag cctctttcgc ttgtcgcttt tcactaaaca tccgacaatt tagcctaacc
    22741 ccggcaaaaa tggagatgcc tatgtccacg acatggtttg taggagccga ctggctcgcc
    22801 gaacatattg atgacccgga aattcagatt atcgatgccc gcatggcgtc gcctggacag
    22861 gaagatcgta acgttgctca ggagtatctg aatggacata ttcccggcgc agtgtttttt
    22921 gatatcgaag cgctttctga tcacacttcc ccgcttccgc acatgctgcc gcgcccggaa
    22981 acgttcgccg tggcgatgcg tgaattaggc gttaaccagg ataagcacct gattgtctat
    23041 gacgaaggta atcttttctc agccccacga gcatggtgga tgctgcgcac ctttggtgta
    23101 gagaaagtgt cgattctggg gggtggactt gcaggctggc agcgcgatga tctgctgtta
    23161 gaagaaggtg cagtagagct gccggaagga gagtttaacg ccgcgtttaa tcctgaagcc
    23221 gtggtgaaag taaccgatgt attattggca agccatgaaa atacggcgca aattattgat
    23281 gcccgcccgg ctgcacgttt taacgcagaa gttgatgaac ctcgcccagg tttacgtcgc
    23341 ggacatattc ccggagcact gaatgttccg tggacggaac tggtgcgcga aggcgaacta
    23401 aaaacgaccg atgaactgga tgcgatattt tttggtcgcg gcgtcagcta cgacaaacca
    23461 attatcgtca gctgcggctc tggtgtaacg gcagccgtgg ttttgttagc actcgcgacg
    23521 ctggatgtgc caaacgtgaa actgtacgac ggcgcatgga gtgaatgggg cgcgcgggca
    23581 gatttaccgg ttgagccagt gaaataagta ttttacaggc aataaaaaac cgccgaattt
    23641 ggcggttttt tattgctggt ctggttcgcg gcctttccag caggttgtat taccgtagta
    23701 atgcaagcgc gtctcagcgg agacaatact cgccagtaac tctctttttg tcaagcaaaa
    23761 gagagttatt attgttctgt tagtgtatta tccactgcgg ccctttccgc cgtctcgcaa
    23821 gcgggcgctg gcttaaggaa aggatgttcc gcgaccgtta gagcaagcgc gtctcagcgc
    23881 ttgctatcgc ggcaatatcg ccagtggtgc tgtcgtgatg cggtcttcgc atggaccgca
    23941 caatgaagat acggtgcttc tgtatcgtac ttattgtttc tggtgcgctg ttttcccaag
    24001 taaataataa ccggagtctc tccggcgaca atttactggt ggttaacaac cttcagagca
    24061 gcaagtaagc ccgaatgccg ccctttaggg cggcatattt tagattatcc ggttttgttt
    24121 aaagtcacgc aaaaaaccac cccagcgacg ttcatagaat ggcacaatat gttcggtaat
    24181 aaagtggcta attccttttt cccctttttt cacctgacaa atatcgattg gttcatcgcc
    24241 aggtaatgta tcggtcgcta cacttcccgt cgcctgaata atttcttcga tatcaccatc
    24301 ggcttcaatg ccaataagta aattaggctg tgcctcttcg ttctctttaa ttgaacaaat
    24361 aaaagcacgc ttcaccggct taatggtttt aaataaggtg gtgagtgaat caatcatttg
    24421 tgctggcggc tctgcgactt ccgataatat cagcgattca ccgccttcca ggatttcctg
    24481 gctgctcagc ggatttccct cttcaccaat caacaaactg atttcacgcg gcataaattc
    24541 tttaccggtc ggcagtttgg cattaaggaa gagcgtttcg ccaagggtca tctcaaacaa
    24601 tgtgcgaacg ggcattacga caaatgcctg ttcgtcttca accgcctgtt gaagcgcttc
    24661 taacgaggtg aaaaaaggaa tgacgctggt gccgtcttct ttttcccagt gctgtaaatc
    24721 aagcgcacta tcttcaacca cagcctcgcc ctgcgccgcc gtaccaggca cccagacggt
    24781 ggattccagt agagtacgga aaaaggccgg gcggtgcgcc ggttcggttg ctgctttttc
    24841 cagcaggtct tcaagttcgt tttttgtttc ggacataaga accacaattc attcaacgtt
    24901 cggggcgcaa atgcctgatg cgctacgctt atcaggccta caagggattc gcaatttgtt
    24961 gaatttgcag aatttgtagg ccggataagg cgtttacgcc gcatccggca tattagttta
    25021 cgccgttaac agattagcta tcgtgcgcac accaagtccc gtagcgcccg cagaccactg
    25081 ttcaaccggc gctttacggt aagtcgccga gcagtcgata tgcagccagc cttgctgata
    25141 gttctcaaca aagtgcgaca ggaagcccgc cgccgtgctc gcgcctgccg gatacgccgc
    25201 gcttccggta ttgttcagtt cggcaaagtt agacggcagc tggctgcggt ggaattccgc
    25261 cagcggcaga cgccagaatg gttcgttctc ttgtgaggca ctcgccagca aacgaccggc
    25321 aagcgcatcg tcaaaactga acagcgcgtg ataatcatta cccagcgcag ttttcgccgc
    25381 cccggtgagg gtcgccgcat caatgatcat ttccggtttc tgcgcactgg catcaatcag
    25441 accatcggca agcaccagac gcccttcggc atcagtgttc atcacttcaa cttttttacc
    25501 gttgcgatag gtgatgatat cgcccagctt gaacgcattg ccgctaatca ggttatccgc
    25561 acagcagagg aacagcttca cgcgcttgtt cagtccgcgc gtaatggcaa atgccagcgc
    25621 cccggtaacc gttgccgcgc cgcccatgtc cgacttcatc gagtccataa acgcagtctg
    25681 tttgatgctg tagccgccgg agtcaaaagt gataccttta cctaccaggc acgcgtacac
    25741 tggcgcttct ttatcgccag ttgggttgta atccagcgcc agcaataccg gagaacgttc
    25801 tgaaccgcgt ccgacggtgt gcagccccat ataaccttgc tcacgcagat cttcgccttt
    25861 ggtgatccga taagtcacac gatcgcccgc gacgttgctg atcagatcaa cagcacgctg
    25921 tgccagttgc gatggtccca attcttctgc cggtgcgttg atggtgtcac gcacccagtc
    25981 gatgatcatc aggcggttat ccagttcctg gcgctgggca tcgtccagat ccggccactc
    26041 cactttacgc gtgcctttcg gggctttgta accttgccag aatgcccagc agcgatccgc
    26101 atcccagcct tcaccgctta actgaacatg cttgatgccc agaccgtcaa tcttgcgcgc
    26161 cgcacgctgg atcagcccca gatcgtctgc cccgttcaaa tgcagggtaa tgccgtcatt
    26221 attaatgctg taagttgctt tttctcccca gcgcgcgtcg gcaggttggg tagagagggt
    26281 aatcttcatc gcttctgtca ttttagttat ccttctttgt aaaagggccg ccagttggca
    26341 gccccaggat ttttccgcta aatgattcgc gttgcagaaa ggcggcaagt gagtgaagcc
    26401 ccaggagcat agataactat gtgactgggg tgaacgagcg cagccaacgc atctgcggcg
    26461 tgaagcatga cgcggaaatt attcggcctc gtccagccag actaacaaaa tcgcttcgag
    26521 gattttctcg ttggatgcct gcgggtcgtc gtcgaaatct tccagatcgc aaatccactg
    26581 atgcatatcg gtgaatcgaa ccgttttcgg atcaagatca ggatacgcat cgtacagtgc
    26641 ttcgccaatt tcgcggctat cggtccactt aagtcccata ctaacctctg ttaatgctca
    26701 cgcgcatggt tgatagtgta acgcgggatt tcgactacta aatcttcgtc ggtaacacgt
    26761 gcctggcagc ttaaacggct ttcgggctcc agtccccagg ctttgtccag catgtcgtct
    26821 tcctgctctg agctttctgg cagcgagtca aaaccttcac gaacgatgca gtggcaggtg
    26881 gtgcaagcac aggatttttc acaggcgtgt tcaatctcga taccgttacg cagagctgcg
    26941 tcgagaatgg tttcaccgct attagcttcc agaacagcgc catcaggaca gagatcctga
    27001 tgaggcaaaa taacaatctt tggcatatta aacctcgtcc acggaatggc ctttcagcgc
    27061 acgacgaacc gactggtcca tgcggcgagc ggcgaaatcc tgggtttgtt tgtctacgtt
    27121 tttaatcgct tgttcgatgg cgtcaacgtc atcgccctgc gccacttcac tcaggtgagc
    27181 ggcagcatcg tcaatgacct gacgttctgc ggcgcttaac agcgcggcat cagcagccag
    27241 cgcgccgtgc agactttcca gcacacgcgc cgcttctact ttttgttctg ccagcattcg
    27301 ggcttttacg tcctgctcgg catagctcat tgagtctttg atcatcgaag cgatttcgct
    27361 atcggtcaga ccgtaagacg gtttgacctg aatagacgcc tcaacgccgg tggatttctc
    27421 catcgccgtc acgctcaaaa gaccgtcggc atcgacctgg aacgtcacgc gaatatgcgc
    27481 accaccagcc ggtagcgccg gaataccacg cagcgcaaaa cgcgccagtg agcggcagtc
    27541 ctgcaccagt tcgcgctcac cctgcattac atggatagac atcgccgtct gaccatcttt
    27601 aaaggtggtg aaatcctgag cgcgggccac cggaatagtg gtattacgcg gaatcacttt
    27661 ctccaccagg ccgcccatcg tttcgaggcc cagcgacagt gggatcacat caagcagcag
    27721 catttcgctg tctggcttgt tacccaccag aatatccgcc tgaatcgccg cgccaatagc
    27781 gacgacttta tccgggtcga tggaagtcag cggtggacga ccgaaaaatt cgcctacccg
    27841 ttcacgcacc agcggcacgc gagtagaacc gcccaccatc accacttcca gcacttcgtc
    27901 agcttctaca cccgcgtctt taagcgcgcg acgacaagcc agtaaggttc gttttaccag
    27961 tggcgcgatc agttcattga attgttcacg gctgatttcg ccctgccagc ccgcaacgtt
    28021 aacggtcacg gagtccgcat cgctcagcgc gattttggct gcaatggcgg catccagcag
    28081 ttcacgctga acgcggttat cgctacgatc aggaatgccc gcctgctcgc gaatgtaatc
    28141 cgccagcaga tggtcgaaat catcgccgcc gagcgcggaa tcaccgccgg ttgccagcac
    28201 ttcaaacacg ccgcgactta agcgcagaat ggaaatatca aacgtcccgc caccgaggtc
    28261 ataaacagcg atcacgcctt cctgaccgga atccagcccg taggcgatag ccgcagcggt
    28321 cggttcgtta agtaagcgca gaacatgcaa ccccgccaga cgcgccgcgt ctttggtgcc
    28381 ctgacgctgg gcatcgtcaa agtacgccgg aacggtgata actacaccat ccagctcgcc
    28441 tgccagggct tcagttgccc gcgccgccag tgctttgagg atgtccgcag aaacgcgcac
    28501 cgggttcagc agccccgccg ccgtttcaat catcggcagg ccgttttcgc tggcctggaa
    28561 ttgataaggc agatgcggat agcgttgctg gatatcagcc agcgagcgcc ccatcaggcg
    28621 tttaacagaa ctaatggtgt tggcggtgtc aagcgctgcg ttagtacgcg cgtcataacc
    28681 caccgaatgc ccttgctgtt gatagtgaac aacagatggc agcaggtgac ggccttcatg
    28741 gtcggctaac gtttcggcct gaccgctgcg cactgtcgcc accagcgagt tggttgtgcc
    28801 caggtcaata ccagccgcca gacgacgctg atgcggcgca gcactcaaac caggttcact
    28861 aatttgtaat aaggccatgt ttagcttcca gaaattaaaa atcgagcagt ttttcttcga
    28921 gttgttcggc actgctccgc agtttatcga gaaaacgcag cttacgcacg gtatccgccg
    28981 ccgcgtccca cgcctcgttg tctaactgtt caaccatcaa ctgatggcgg gtatcaaaca
    29041 tctttttcac acgtttgata aagctttcca gccgcgcttc atctttcgcc tgttcgatct
    29101 cgtccagctc ttcgcgcagc tccaactgtt ccatcaggaa cgcggtgtcg cgcacagtat
    29161 gctgctcgct ggcgagatca aaaccgtgta aagaaagcaa atattccgcg cgcattaacg
    29221 gatgacgcag cgtttgccag gcctggttaa tggttgcaga ttgctgtacg gcggcgagtt
    29281 gttccgcctg gcttccgctg gcgaatttat caggatgata ctgacgttgt agatcctgaa
    29341 aacgcaggct cagcgcctgg gtatcgagtt gataacgggc aggcaagcca aagaaggtga
    29401 agtaatccat aacattctca gggctgcgat ccgccgcgat aacggcgaat caggtaaaac
    29461 aaaccccacg cgcaggcgac cacggtgggg ttatcggtat gcgcatcaaa cgtggaagct
    29521 ttcgccgcaa ccacactcat ctttgacgtt cgggttggtg aatttaaacc cttcgttcag
    29581 gccttctttt acgaagtcca gctgcgtacc gtccagaaat tgcaggcttt tgccatcgac
    29641 cacgactttc acgcctttgt cttcaaacac gatgtcttcc ggcgtcggtt cgtcaacaaa
    29701 ttccagtaca taagccatac ctgaacatcc ggaggttctc acgcccagac gcaggccaaa
    29761 ccctttgccg cggttagcca gaaaggtatt tactcgcgct gctgcactgt cgctcagtgt
    29821 aatcgacata accaaacctc aactcttatt ttgcttcacg tttgctttta tagtccgcaa
    29881 tggcggcttt gatcgcgtct tctgccagaa tagaacagtg aattttcacc ggcggcagtt
    29941 caagttcttc agcaatatcg gtatttttga tcgcctgcgc ttcgtcgaga gacttccctt
    30001 tcacccattc ggtgaccagg gagctggaag cgatagcaga accgcagccg taagttttaa
    30061 aacgcgcgtc ttcaatgata ccttcatcgt tgactttaat ctgcaacttc atcacgtcgc
    30121 cacaggccgg tgcccccacc atgccgctgc cgacgttctc gtcgttgttg tcaaaggaac
    30181 ccacgttacg cggattctcg taatggtcga taactttttc gctgtaagcc attataaatt
    30241 ctcctgattc cgataccgat taatgatgag cccattcgat gctgttcaga tccacgccct
    30301 gcttgtacat ttcccacagc ggagaaaggt cacgcagacg accgatggat ttacgaacta
    30361 actcgatggt gtagtcgatc tcttcttcag tagtaaaacg acctaaagag aaacggatag
    30421 agctatgtgc cagctcgtcg ttcagcccca gcgcgcgcag cacgtaggac ggttcgaggc
    30481 ttgctgacgt acaggcggaa cctgaagaaa ctgcgaggtc tttcagcgcc ataatcagcg
    30541 actcaccttc aacgtagttg aagctgacgt tgagaatgtt cggcgcaccg tgttccaggt
    30601 caccgttcag gtaaacttct tcgatatctt tgatgccgtt ccacagacgg ttacgcaggc
    30661 cgcgcagacg ttccatctcg gtcgccatct cttcttttgc gatgcgatag gcctcgccca
    30721 ttccgacgat ctggtgaaca ggcagagtgc cggaacgcat accgcgctcg tgaccgccgc
    30781 cgtgcatttg cgcttcgatg cgtacgcgcg gtttacgacg tacatacagc gcaccgatac
    30841 ctttcgggcc atagattttg tgaccggaga aagacatcag gtcaactttc aactggctca
    30901 ggtcgatagg cagtttgccc acgctctggg ttgcatcaac gtgatagata atgccacgag
    30961 cacggcacat ttcgccgata gccgcgatat cctgcaccac gccgatttcg ttatttacgt
    31021 gcatgatgga aacaaggatg gtgtcatcac gcatcgctgc ttcaagttct ttcagatcga
    31081 taatgccgtt acgctgcggt gccaggtagg tgacttcaaa accttcgcgc tccagctgac
    31141 ggcaggtatc cagtaccgct ttgtgttcgg ttttgctggt gatgatgtgc ttgccttttt
    31201 tctgataaaa gttggctgca cctttgatcg ccaggttgtc agattcggtt gcaccagagg
    31261 taaagacgat ttcacgcgga tcagcgccga ccagatcggc aatctgatta cgggcgatat
    31321 ctaccgcttc ttcagcctgc cagccgaaac ggtgagaacg ggaggccggg ttaccaaagg
    31381 ttccgtccat cgtcataaac tgcatcattt tctcggcaac acgcgggtcc accggcgtgg
    31441 ttgcggagta gtcgagataa atcggtaatt tcattgctct ataaactccg tacatcactc
    31501 aatgcaagga accaggctac cggctggatg tacgaccgtg tttacggagc gcgatacgcc
    31561 ccggcctgat tctaatatct tgttatcttt aacctaactg tttcgggttt cagcaagttg
    31621 acaaacttac gaatctccgg gagcttacat aagtaagtga ccggagtgag tatgtgcagt
    31681 caacgcagat gcaacacgaa acaggacggt taaattaagc gcgtaactta acgtcgatcg
    31741 cgtcttgtat gcgggtgcgt ggcgcgtcgt gagtatgctg acgaccagac acatccagca
    31801 cttcctggtt attaaccagt tcgcctaaag taatgttgtt gagaaaaccg gtgagacggt
    31861 cgctcaaatc acgccacagc gcgtgggtca ggcatttatc gccgccctgg caaccgcctt
    31921 taccctgaca acgggtagca tctacagatt cgtcaacggc gctaatcact tcgccaacgg
    31981 cgatgctgct ggcatctttg cctaacagat aaccaccgcc tggtccacgt acgctggaaa
    32041 ccagaccatt tttacgcaga cgggaaaaca gttgttccag ataagaaagg gaaattccct
    32101 gacgttcgga aatatcagcc aacggtaccg ggcccgcttc agagttgagc gcaacgtcaa
    32161 gcattgcggt cacggcatag cgccctttag atgtcagtct catgtcttac ttcacctcaa
    32221 actcgcccct gcccggggtt ttttattgta aaagtggggg tattgcatag cagggtcaag
    32281 tctgacattc ccgagtaaat tggtcaacta tttacttgac tgatttagtc gggtatttaa
    32341 ccttcagtgc cattttttta tcgtggcgtt tgtacctgtt gtccttccag accatgggcg
    32401 ggttgagggc aattattcgc attagcgctg gcctcttccc ctcaccctaa ccctctcccc
    32461 gaaggggcga ggggaccgtc cgtgcattta tctggcttag ccctcaggcc ttattacctt
    32521 tattctgctg ctctatagaa gccagaatcc cgcgcaggat attcaattcc tggctttccg
    32581 ggcgcgcacg ggtaaacaga cggcgcaatt tattcatcac ctgccccgga tggttttcac
    32641 ggataaaacc ggttgccagc agcgtttgtt ccagatgacc ataaaaacgc tccagatcat
    32701 cgaccagcgg atacggcgtc tcttcatgct cgacctgctc gccattttcc tgagttgcca
    32761 gccaggccat acgcacttca taggcaataa cctgaaccgc cattgccaag ttcagcgaac
    32821 tgtattccgg gtttgccgca atcgcgacat gataatggca tttctgcaac tcttcattgg
    32881 tcaagccgac gcgctcacga ccaaacacca gcgccaccgg ggtatttgcc gcttcagcga
    32941 cgcttttcag accgcattcg cgcgggtcga gcatcggcca cggcagcgtg cgggagcgtg
    33001 cgctggtgcc caccaccagg ctacaaccgg ctaacgcttc gtcgagcgta tcgacgatgt
    33061 gagcattacc aatcacatcg ctagcccctg ctgccagggc aatcgcctgg gagtcgggtt
    33121 tcaccagtgg attaaccagc cacagattgg ttaatcccat tgttttcatg gcacgggcaa
    33181 cagaacccat attgccggtg tgtgacgtct ccaccagcac aattcgaata ttttgcagca
    33241 ttgtctttct tcatctaaag attattcacg catcttatca taaaacgaag acagatgccg
    33301 atctcgctgc tatactctgc gccgttttcc cgttctttaa catccagtga gagagaccga
    33361 tgcatccgat gctgaacatc gccgtgcgcg cagcgcgcaa ggcgggtaat ttaattgcca
    33421 aaaactatga aaccccggac gctgtagaag cgagccagaa aggcagtaac gatttcgtga
    33481 ccaacgtaga taaagctgcc gaagcggtga ttatcgacac gattcgtaaa tcttactcac
    33541 agcacaccat catcaccgaa gaaagcggtg aacttgaagg tactgatcag gatgttcaat
    33601 gggttatcga tccactggat ggcactacca actttatcaa acgtctgccg cacttcgcgg
    33661 tatctatcgc cgttcgtatc aaaggccgca ccgaagttgc tgtggtatac gatcctatgc
    33721 gtaacgaact gttcaccgcc actcgcggtc agggcgcaca gctgaacggc taccgtctgc
    33781 gcggtagcac cgctcgcgat ctcgacggta ccattctggc gaccggcttc ccgttcaaag
    33841 caaaacagta cgccactacc tacatcaaca tcgtcggcaa actgttcaac gaatgtgcag
    33901 acttccgtcg taccggttct gcggcgctgg atctggctta cgtcgctgcg ggtcgtgttg
    33961 acggtttctt tgaaatcggt ctgcgtccgt gggatttcgc ggcaggcgag ctgctggttc
    34021 gtgaagcagg cggcatcgtc agcgacttca ccggtggtca taactacatg ctgaccggta
    34081 acatcgttgc tggtaacccg cgcgttgtga aagccatgct ggcgaacatg cgtgacgagt
    34141 taagcgacgc tctgaagcgt taatgactca ggcgggtgat gtcactcacc cgccctcgcc
    34201 tttcaggcgc tattctgaaa tacttcctca ccgctttact ttctttcgtc actctcccac
    34261 catctttccc cgattaatgg atgataaaga agtcacaagg aaaggttatg gcgctgccag
    34321 tgaacaaacg cgttctcaaa attctgttta ttctctttgt tgttgccttc tgcgtttatt
    34381 tagtgccgcg cgttgccatc aacttcttct attatcctga cgataaaatt tacggtcccg
    34441 acccctggtc ggcggaatcc gtcgaattta cggctaagga cggtactcgt ctgcaaggct
    34501 ggtttattcc ttcttcgacg ggccctgctg acaatgccat cgcaaccatc attcatgctc
    34561 acggcaatgc cggaaatatg tccgcccact ggccgctggt cagttggtta cccgagcgta
    34621 atttcaacgt ttttatgttt gattatcgcg ggtttggtaa atcaaaaggc acgccgtccc
    34681 aggccggatt gctggacgat acgcaaagtg ccatcaatgt ggtgcgccat cgcagtgatg
    34741 taaacccaca acggctggtg ctgttcgggc agagtattgg cggggcgaat attcttgctg
    34801 ttattggtca ggatgatcgt gaaggcatac gtgcggtgat cctcgactcc acatttgcct
    34861 cttatgcaac catcgccaat cagatgatcc ccggcagtgg ctacttactt gatgagagtt
    34921 acagcggcga aaattatatc gccagcgtca gcccgatccc gcttttactc attcacggta
    34981 aagctgatca cgttatccca tggcagcaca gcgaaaagtt gtatagcctg gcaaaagaga
    35041 caaaacggtt gatcctaatc ccggatggcg aacacattga tgctttttcc gatcgtcacg
    35101 gcgatgttta tcgcgaacag atggtggact ttatccttag cgcgttgaat ccgcagaact
    35161 aacccatgat cgctagcacg ataatcattc acaaaaccac cttaagacat gctaatccac
    35221 tggtcagaac agtttaagat gagaaaaatt ctgtgacgct tgccaacatt tctgatgatt
    35281 agcattccct tcgccatttc cctgagcaaa ctttagctat tcttatcaat tatgcttatg
    35341 ggagatctac agatgatgcc tacgcttgct ccaccatctg tcctttcggc tccccagcgc
    35401 cgctgccaga tcttgctgac gctctttcag ccggggttga ccgccaccat ggcaaccttc
    35461 agcgagctta atggtgtgga tgatgatatt gccagtcttg atatcagcga aacaggacgg
    35521 gagatcctgc gctatcatca actcacactg acgactggtt atgacggtag ctaccgggtt
    35581 gaaggtacag tgcttaacca acgtttgtgt ttatttcact ggctacgacg tggtttccgt
    35641 ctgtgtccgt catttattac cagccaattc acccccgccc tgaagagtga actgaagcgg
    35701 cgcggaattg cgcgtaactt ttacgacgat accaatctac aagcgttagt gaatctctgc
    35761 tcccgacggc tgcaaaaacg ctttgaaacg cgcgatattc atttcctgtg tctgtatctg
    35821 caatattgtt tgctgcaaca ccacgctgga attacgcctc agtttaatcc gctccaacgt
    35881 cgctgggcgg aatcctgcct tgaatttcag gtagcgcagg aaattggacg ccactggcag
    35941 cgtcgggcgc tccagcctgt accgcctgat gagccactgt ttatggcatt acttttttcc
    36001 atgttgcggg ttcccgatcc attgcgtgat gcgcatcagc gagacagaca attgcgtcag
    36061 tctatcaaac gtctggtaaa ccattttcgt gagctgggaa atgttcgttt ttatgatgaa
    36121 caggggttat gcgatcagct ttatacccac ctcgcccagg cgttaaatcg cagtttgttt
    36181 gccatcggta ttgataatac cctgccggaa gagttcgcca gactgtaccc acgcctggtg
    36241 cgcaccaccc gcgcggcgct ggccggattt gaaagtgaat acggcgtcca tctttctgat
    36301 gaggaaagcg gtctggtcgc ggtgattttc ggtgcctggc taatgcagga aaacgatctg
    36361 catgaaaaac agattattct actgaccggg aatgatagcg agcgagaagc gcagattgag
    36421 cagcagttac gcgaactaac gttactgccg ctcaacatta agcatatgtc ggtaaaggca
    36481 tttttgcaga caggcgctcc gcgtggcgcg gcactgatta ttgcgcctta taccatgcca
    36541 ttaccgctct tttcaccacc gctgatctat acggacctga cgctgacaac acatcaacag
    36601 gagcagatcc gcaaaatgct cgaatcagca tgagggaaca actttcgggc gcaaaaacat
    36661 tgccggaagc gccaccagcg ccattaccca gaacacgccg tggcccagat attgatacag
    36721 gaaaccggcg aaaacggtca tgatagcgat actgccgccc atcgcgacgg cagagtaaac
    36781 cgcctgtaaa cggatgactt cgctaccctg gcgaacagca atatagcgca tggcggccag
    36841 gtggcagacc gtgaaggtgc cgcaatgcaa aatttgcacc actatcaacc acggcaacgc
    36901 cgtagttgct cccataatgc cccagcgcac tacgccgcaa atcgccgaga tcaacagcat
    36961 atcgcgagca ctacaacggc ggaaaagttt attactcagc gcaaagataa tgacttccgc
    37021 caccacgccc agcgaccaca aataccccac cgccgaggcc gagtagccag ctgcctgcca
    37081 gtaaatggcg ctaaaaccgt aataggccgc atgtgccccc tgcaataaac aaacgcaggc
    37141 cagaaagcgc cagttctggc gaaccagcgc caaccacgca gaccaaccgg tgctctcctg
    37201 ctggcgactt gccccttgtg gctgaatcgt cggacggatg agaaagccga gcagcatgga
    37261 tgccacgccc aacgtcaaca gcgcgaggat cacccgataa tcaaacatag tgaccagttt
    37321 gcccgtcagc gccgagccaa tgacaaacgc caccgagccc cacagtcgca ctttgccgta
    37381 atcaagcggg aactgctttt gccacgtatt cgccagtgca tcggtcaacg gtaccagcgg
    37441 tgagaaaaag aggttaaagc caatcatcac cagcatcagc cacgctacgt gcgcccccgc
    37501 ccagaaggcg acagcaaaga gaagtgtcag cagtgccagc acgcgcaagg cggaaatcag
    37561 gcgggaagga tcgctgacgc ggggcgcgat gagcaaactc ccgagaaaac gggcaaccag
    37621 acctgccccc aataacaggc cgatggtttc tggcgttaaa ccaatccctt taagccagac
    37681 gctccagaaa ggtagaaaaa tgccgtaact aaaaaagtat gtgaaatagc cgagcgccaa
    37741 ccagcgcgtg gattgcaaaa ccatgagtcc ctcccgtcag gaggcgttag tctggggtta
    37801 atcgaatgtt ggtgcaagtt gaaaaataag aatattcgag cctgcttccc gatgtctgta
    37861 acgccagttc acaagcacta aaaaactgcg atccgcgacg catgttgcaa aaataatgcg
    37921 taattatgcc gttacgcttg ccaaacgttc ctgcacaatg gcgatgaaat cgcgtaatgc
    37981 cggcttcatc tcccctttct tccacgccat cagtaaagca atggaaggca catttccggc
    38041 aatgggacga aaaacaacct gcccggtatt aaaattattc atataaccgg gtatcaaagt
    38101 gacgcccagc cccatgccca ccagattcat ggtcaccaga atattcgttg ccacctggac
    38161 gatatttggc tggctgtttt cttgcgcaaa ccacgcttta acgatcggcg caagcgaacc
    38221 ggaatacgcc ggatcggtac tgacgaaatt cacgccatcc agttacgcgg cggtgatctc
    38281 tttttcatgt gctaaagggt gatcaaccgg taacaccacc actaatggtt cgtcaaaaag
    38341 ctccagataa tcaatctccg ggctataaac aggatggcgc attaagccga cgtcgagttc
    38401 accacggcga attttttcct cctgttgcgt ggtgattaaa ctcaccagct caatcaaggt
    38461 gtctggctgt ctgagacgaa acattggtaa tacttttggc aataaattca cttccgccga
    38521 tggcacaaag ccaatggtta attgtctgtc ttcctgaaca attttccgcg cccgtaattt
    38581 ggcattttcc gcttgttcga ggattgccag cgcgtcctgg agaaaacatt ctcccgccgc
    38641 cgtcaacgcg actttgcgct tatccctcac cagtaacgga acaccgacac agttttcaag
    38701 atcgcggatc tggctgctta acgaaggctg tgaggtatgc agtttttccg ccgcacgggt
    38761 aaagttcagt gcctgcgcca ctgcgacgaa atagcgtaaa tgccgtagtt ccatcacctt
    38821 ccccttgtta tcgaaaaaac gtctcagctg gtagaaatta actatttcac atattagcaa
    38881 ccaaccaaca acatccttat gacacaaaaa tagaaggtca atacatctta tctttcagga
    38941 ttaaaaaata tgaccacacc ctcagattta aacatttacc aactgattga tacccaaaat
    39001 ggtcgggtca ctccgcgtat ttataccgac ccagatattt accaactgga gcttgaacgt
    39061 attttcggcc gttgctggtt atttctcgcc cacgaaagcc agatcccaaa acctggtgat
    39121 ttctttaaca cctacatggg ggaagatgcg gttgtcgtag tgcgtcagaa agacggcagc
    39181 attaaggcgt ttctcaacca atgccgccac cgggccatgc gtgtgagtta tgcagattgc
    39241 ggcaacactc gcgcctttac ctgtccgtat cacggctggt cttatggcat taacggcgag
    39301 ttgatcgatg taccgctgga acctcgcgcc tacccacaag ggttgtgtaa atcccactgg
    39361 ggactaaacg aagttccttg tgtggagagt tataaagggc tgatttttgg caactgggat
    39421 accagcgcac cgggcctgcg tgattacctg ggtgacattg cctggtatct ggatggcatg
    39481 ctggatcgtc gcgaaggcgg caccgaaatt gtcggcggcg tacaaaagtg ggtgatcaac
    39541 tgtaactgga aattcccggc agagcagttc gccagtgacc agtatcatgc tctgttcagc
    39601 catgcttctg ccgttcaggt attaggggcg aaagatgatg gcagcgataa gcgcctcggt
    39661 gatggacaaa ccgcccgccc ggtgtgggaa accgccaaag atgcgctgca atttggtcag
    39721 gacggtcacg gtagcggttt cttctttact gaaaaaccgg atgctaatgt ctgggtcgat
    39781 ggcgcagttt caagctatta ccgcgaaacc tatgccgaag cagaacaacg tttaggtgaa
    39841 gttcgcgccc tgcgcctggc gggtcataac aatattttcc ccacgctttc atggctcaac
    39901 ggcactgcca cgctccgcgt ctggcatccg cgcggccctg atcaagttga agtgtgggcg
    39961 ttctgtatta ctgacaaagc cgcctccgat gaagttaaag ccgcttttga aaacagcgcc
    40021 actcgtgctt ttggtcctgc tggttttctc gagcaggatg actcggagaa ctggtgtgaa
    40081 atccagaaat tgcttaaagg ccaccgcgcc cgcaacagca aactgtgtct ggaaatgggg
    40141 cttggtcagg aaaagcgccg cgacgacggc attcctggca ttactaacta tatcttttca
    40201 gaaactgccg ctcgtggaat gtaccaacgc tgggccgatc ttctgagtag cgaaagctgg
    40261 caggaagtgc tcgataaaac cgccgcttac cagcaggagg tgatgaaatg agtgcgcaag
    40321 tttcactaga gttacatcac cgcattagcc agtttctctt tcacgaagcc agcttactgg
    40381 acgactggaa atttcgtgac tggctggagc agctcgacaa agagattcgt tacaccatgc
    40441 gcaccacagt taacgcgcaa acacgcgacc gccgcaaagg cgtccagcca ccgacaacct
    40501 ggatttttaa tgacaccaaa gaccagctgg agcggcgaat cgcccgtctg gaaacgggca
    40561 tggcctgggc agaagagccg ccgtcacgca cccgtcactt aatcagcaac tgccagataa
    40621 gcgaaaccga catcccaaac gtatttgctg tgcgggtaaa ttatctgctt tatcgggcac
    40681 aaaaagagcg cgatgaaaca ttctatgttg gaacgcgttt cgacaaagtt cgccgtctgg
    40741 aagatgacaa ctggcgcttg ctggaacggg atatcgtcct ggatcaagcg gtaatcactt
    40801 cccataacct gagtgtactg ttctgatgaa tcgaatttat gcgtgtcccg ttgcggatgt
    40861 gccggagggt gaggctctcc ggatcgatac ctcgcccgtc atcgccctgt tcaacgttgg
    40921 cggcgagttt tatgccatta acgatcgttg cagccatggt aatgcgtcga tgtcagaagg
    40981 gtatctggaa gatgacgcca cggtggagtg cccgctacac gccgccagtt tttgcctgaa
    41041 aacggggaaa gcgttatgcc tgcccgccac cgatccgctc accacttatc cagtacacgt
    41101 tgaaggtggt gacattttca tcgacttacc ggaggcgcag ccatgagcga tctgcataac
    41161 gagtccattt ttattaccgg cggcggatcg ggattagggc tggcgctggt cgagcgattt
    41221 atcgaagaag gcgcgcaagt tgccacgctg gaactgtcgg cggcaaaagt cgccagtctg
    41281 cgtcagcgat ttggcgaaca tattctggcg gtggaaggta acgtgacctg ttatgccgat
    41341 tatcaacgcg cggtcgatca gatcctgact cgttccggca agctggattg ttttatcggc
    41401 aatgcaggca tctgggatca caatgcctca ctggttaata ctcccgcaga gacgctcgaa
    41461 accggcttcc acgagctgtt taacgtcaac gtactcggtt acctgctggg cgcaaaagcc
    41521 tgcgctccgg cgttaatcgc cagtgaaggc agcatgattt tcacactgtc aaatgccgcc
    41581 aggtatcctg gcggcggtgg cccgctgtac accgccagta aacatgctgc aaccggactt
    41641 attcgccaac tggcttatga actggcaccg aaagtgcggg tgaatggcgt cggcccgtgt
    41701 ggtatggcca gcgacctgcg cggcccacag gcgctcgggc aaagtgaaac ctcgataatg
    41761 cagtctctga cgccggagaa aattgccgcc attttaccgc tgcaattttt cccgcaaccg
    41821 gcggatttta cggggccgta tgtgatgttg gcatcgcggc gcaataatcg cgcattaagc
    41881 ggtgtgatga tcaacgctga tgcgggttta gcgattcgcg gcattcgcca cgtagcggct
    41941 gggctggatc tttaaggaag cacgatgaaa gaaaaaacga tcattattgt cggtggcggg
    42001 caagcggcgg caatggctgc ggcctcgcta cgccagcaag ggttcaccgg tgagctgcat
    42061 ctgttttccg atgagcgaca tcttccttat gaacgacctc cactctcgaa atccatgttg
    42121 ctggaagatt ccccgcagtt acagcaggtg ttacccgcta actggtggca ggaaaacaat
    42181 gttcatctgc attccggtgt aaccatcaaa acgctgggtc gcgacacacg agagttagtg
    42241 ttaaccaacg gcgaaagctg gcactgggat cagcttttta tagcaatcgg cgcggcagct
    42301 cgaccgctgc cgttgcttga tgcactggga gaacgctgct ttaccctacg ccatgccggt
    42361 gatgccgcca gactgcgaga agttctgcag cccgaacggt cagtcgtgat tgtcggtgcc
    42421 ggaactattg gtctggaact ggctgccagc gccacgcagc gcagatgtaa ggtgacagtg
    42481 attgaactgg cggcaaccgt catgggccgt aatgcaccac cgcccgtgca acgctatctt
    42541 ttacagcgcc accagcaggc tggtgtgcgc attctgctca ataatgccat tgaacatgtg
    42601 gtcgatggtg aaaaagtaga actgacgctg caaagtgggg aaacgcttca ggctgatgtg
    42661 gtgatttacg gtattggtat cagcgccaac gagcaactgg ctcgcgaggc caaccttgat
    42721 actgccaatg gcattgtcat tgatgaggct tgccgcacct gcgatcccgc gatctttgcc
    42781 ggtggcgatg tggcaatcac tcgtcttgat aatggtgcac tacaccgctg cgaaagctgg
    42841 gaaaacgcca ataaccaggc gcaaattgcc gctgccgcaa tgttagggct accgctaccg
    42901 ctactgccgc cgccgtggtt ctggagcgat cagtacagtg ataacttaca gtttattggc
    42961 gatatgcgtg gcgatgactg gctttgtcgt ggcaacccgg aaactcagaa ggcgatttgg
    43021 tttaatctgc aaaacggcgt gcttatcggt gcggtcacgc tgaatcaggg gcgtgagatt
    43081 cgcccaattc gcaaatggat ccagagcggc aaaacgtttg atgcgaaact gctgatagat
    43141 gagaacatcg cgcttaaatc actgtaacca ggataattag cgaatatctc aacgcctggg
    43201 gcgtggcgag gtgcaagagt gtgtattacg tttaaatcac attatcttgc aaagggattg
    43261 gttatgaaca cacta
//
LOCUS       DADMKF010000015        42852 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.015, whole genome shotgun sequence.
ACCESSION   DADMKF010000015 DADMKF010000000
VERSION     DADMKF010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 42852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42852
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.015"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            <1..1571
                     /locus_tag="JNC24_000765"
     CDS             <1..1571
                     /locus_tag="JNC24_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HAY9157593.1"
                     /translation="NHQDKHSDKVNSHQHHGKVDKKYRSNTVEVNMLNDFSQIDNDLG
                     LNIIYFLQHGHWRVNEHSLQMEKIWFYNSEPSIDIQEYNSFADNTTNTFIFTIIPDNN
                     HVIKLSSPITVKIECKDGYYFINFSGDKSDIIYKSDITYKVNGLSIIPENFFTLLSGN
                     FKADWRWDVSKETFPKDKFDSYVKSVFSKIDFYKQCGVINPQNANTAYFGDTDGRVGA
                     VLYALLVSGHIGIREKGWSLLCELLKHENMASSAYIHKNNKLLYNLLNTRDMILNELH
                     QHVFLKDDAITPCIFLGDHTGDRFSTIFGDKYILTLLNSMRNMDGNKDSRINKNVIVL
                     AGNHEINFNGNYTARLENYKLSAGDTYNLIKTLDVCHYDSETKVLTSHHGIIRNEENE
                     CYCLGALQVPFNQMKDPIDPEELANIFNKKHKQHMDDRLIHLIRSNAVASTPVYENYF
                     NNTTAFRPKPEDIFKCGQTLKKTKQKYGHYGLGVDQHQKIDNYTMGLNSWKIAPNERG
                     DKKGVPGLSCFQPQ"
     gene            1833..3455
                     /locus_tag="JNC24_000766"
     CDS             1833..3455
                     /locus_tag="JNC24_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HAY9157594.1"
                     /translation="MKIGTVAGTNDSTTTITTNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIQDDKISFTIQTNHSEKYSMLSGMRAHILRR
                     NNCYQFIVTINSKNYGCPLDNTDINWCSIVYLLNNMTVNDNANDVAVTESYKPIWNWK
                     ISQYNVFDIKFETMIKPQFADRTYFSNCSPVDPTSTRPTYFGDTDGSVGAVLYALFAT
                     GHLGIMAEGENFLSQLLNIEDEVLNVLLRENFNEQLDTNVNIIISILNRRDIVLESLQ
                     PYLVINKDAVTPCTFLGDQTGDRFSNICGDKFIIDLLKRIMSINENVHVLAGNHETNC
                     NGNYMQNFTRMKPLDEDTYSGIKDYPVCFYDPKYKIMANHHGITFDDQRKRYIIGPIT
                     VSSDEMTNALDPVELAAIINKKHHAVINGKKFKNSRAISCRSFNRYFSVSTDYRPKLE
                     ALLACSQMLGINQVVAHNGNGGRECIGETGTVLGLNARDSKHAGRMFSMHNCQINPGA
                     GPEITTPWKSYQHEKNRNGLMPLIRRRTMLQL"
     gene            3821..4888
                     /locus_tag="JNC24_000767"
     CDS             3821..4888
                     /locus_tag="JNC24_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HAY9157595.1"
                     /translation="MDKSKRHLAWWVVGLLVVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQSELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELSWPDDLVVRLPQ"
     gene            4885..7620
                     /gene="rbbA"
                     /locus_tag="JNC24_000768"
     CDS             4885..7620
                     /gene="rbbA"
                     /locus_tag="JNC24_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HAY9157596.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKNVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVMVLGGDMRDPRHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRSQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            7620..8744
                     /locus_tag="JNC24_000769"
     CDS             7620..8744
                     /locus_tag="JNC24_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HAY9157597.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKIWSMGLVVLVVSGLSLVLMAKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            8817..9090
                     /locus_tag="JNC24_000770"
                     /pseudo
     CDS             8817..9090
                     /locus_tag="JNC24_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
     gene            9087..9446
                     /locus_tag="JNC24_000771"
     CDS             9087..9446
                     /locus_tag="JNC24_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HAY9157598.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(9566..9967)
                     /gene="nikR"
                     /locus_tag="JNC24_000772"
     CDS             complement(9566..9967)
                     /gene="nikR"
                     /locus_tag="JNC24_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HAY9157599.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(9973..10779)
                     /gene="nikE"
                     /locus_tag="JNC24_000773"
     CDS             complement(9973..10779)
                     /gene="nikE"
                     /locus_tag="JNC24_000773"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HAY9157600.1"
                     /translation="MTLLNVSGLSHHYAHGGFNGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPAQGNISWRGEPLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQRV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIVETQVVGDKLTFSSDAGRVLQNAVLPAFPVRRRTTEKV"
     gene            complement(10776..11540)
                     /gene="nikD"
                     /locus_tag="JNC24_000774"
     CDS             complement(10776..11540)
                     /gene="nikD"
                     /locus_tag="JNC24_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HAY9157601.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKAVYPCALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGK
                     IVEQGDVETLFNAPKHAVTRSLVSAHLALYGMELAS"
     gene            complement(11540..12373)
                     /gene="nikC"
                     /locus_tag="JNC24_000775"
     CDS             complement(11540..12373)
                     /gene="nikC"
                     /locus_tag="JNC24_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HAY9157602.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSR
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            complement(12370..13314)
                     /gene="nikB"
                     /locus_tag="JNC24_000776"
     CDS             complement(12370..13314)
                     /gene="nikB"
                     /locus_tag="JNC24_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HAY9157603.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGGWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            complement(13314..14888)
                     /gene="nikA"
                     /locus_tag="JNC24_000777"
     CDS             complement(13314..14888)
                     /gene="nikA"
                     /locus_tag="JNC24_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter, nickel/metallophore
                     periplasmic binding protein"
                     /protein_id="HAY9157604.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALSKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTPQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKELLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            complement(14999..15586)
                     /gene="acpT"
                     /locus_tag="JNC24_000778"
     CDS             complement(14999..15586)
                     /gene="acpT"
                     /locus_tag="JNC24_000778"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HAY9157605.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            complement(15641..16690)
                     /locus_tag="JNC24_000779"
     CDS             complement(15641..16690)
                     /locus_tag="JNC24_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAY9157606.1"
                     /translation="MEIPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLELM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGVYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            16822..18039
                     /locus_tag="JNC24_000780"
     CDS             16822..18039
                     /locus_tag="JNC24_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY9157607.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVNY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     RVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKVGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            complement(18043..18600)
                     /gene="dcrB"
                     /locus_tag="JNC24_000781"
     CDS             complement(18043..18600)
                     /gene="dcrB"
                     /locus_tag="JNC24_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HAY9157608.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(18673..19338)
                     /locus_tag="JNC24_000782"
     CDS             complement(18673..19338)
                     /locus_tag="JNC24_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HAY9157609.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSILGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNILLKRLADKSEINAL
                     QAS"
     gene            19559..19804
                     /gene="tusA"
                     /locus_tag="JNC24_000783"
     CDS             19559..19804
                     /gene="tusA"
                     /locus_tag="JNC24_000783"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HAY9157610.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            complement(19906..22104)
                     /gene="zntA"
                     /locus_tag="JNC24_000784"
     CDS             complement(19906..22104)
                     /gene="zntA"
                     /locus_tag="JNC24_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HAY9157611.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSSPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAA
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTKLNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(22178..22804)
                     /locus_tag="JNC24_000785"
     CDS             complement(22178..22804)
                     /locus_tag="JNC24_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HAY9157612.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIIRSLYL"
     gene            22945..23304
                     /locus_tag="JNC24_000786"
     CDS             22945..23304
                     /locus_tag="JNC24_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HAY9157613.1"
                     /translation="MSKPPLFFIVIIGLIVVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            complement(23307..23576)
                     /locus_tag="JNC24_000787"
     CDS             complement(23307..23576)
                     /locus_tag="JNC24_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HAY9157614.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(23566..24162)
                     /gene="rsmD"
                     /locus_tag="JNC24_000788"
     CDS             complement(23566..24162)
                     /gene="rsmD"
                     /locus_tag="JNC24_000788"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HAY9157615.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRAVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQREAQGESDAD"
     gene            24312..25808
                     /gene="ftsY"
                     /locus_tag="JNC24_000789"
     CDS             24312..25808
                     /gene="ftsY"
                     /locus_tag="JNC24_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HAY9157616.1"
                     /translation="MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     VKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVVESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVKAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            25811..26479
                     /gene="ftsE"
                     /locus_tag="JNC24_000790"
     CDS             25811..26479
                     /gene="ftsE"
                     /locus_tag="JNC24_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HAY9157617.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSHRMLTLSDGHLHGG
                     VGHE"
     gene            26472..27530
                     /gene="ftsX"
                     /locus_tag="JNC24_000791"
     CDS             26472..27530
                     /gene="ftsX"
                     /locus_tag="JNC24_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HAY9157618.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINDLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            27775..28629
                     /gene="rpoH"
                     /locus_tag="JNC24_000792"
     CDS             27775..28629
                     /gene="rpoH"
                     /locus_tag="JNC24_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007899894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HAY9157619.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMQGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            28900..30003
                     /locus_tag="JNC24_000793"
     CDS             28900..30003
                     /locus_tag="JNC24_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HAY9157620.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(30202..30585)
                     /gene="panM"
                     /locus_tag="JNC24_000794"
     CDS             complement(30202..30585)
                     /gene="panM"
                     /locus_tag="JNC24_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HAY9157621.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
     gene            31009..32118
                     /gene="livk"
                     /locus_tag="JNC24_000795"
     CDS             31009..32118
                     /gene="livk"
                     /locus_tag="JNC24_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HAY9157622.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINAKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            32166..33092
                     /gene="livH"
                     /locus_tag="JNC24_000796"
     CDS             32166..33092
                     /gene="livH"
                     /locus_tag="JNC24_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HAY9157623.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYGVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            33089..34366
                     /locus_tag="JNC24_000797"
     CDS             33089..34366
                     /locus_tag="JNC24_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivM"
                     /protein_id="HAY9157624.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDSASDVR
                     WQWVFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLAVS
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTFFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAILLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            34363..35130
                     /gene="livG"
                     /locus_tag="JNC24_000798"
     CDS             34363..35130
                     /gene="livG"
                     /locus_tag="JNC24_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HAY9157625.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRNQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            35132..35845
                     /gene="livF"
                     /locus_tag="JNC24_000799"
     CDS             35132..35845
                     /gene="livF"
                     /locus_tag="JNC24_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HAY9157626.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            36129..37193
                     /gene="ugpB"
                     /locus_tag="JNC24_000800"
                     /pseudo
     CDS             36129..37193
                     /gene="ugpB"
                     /locus_tag="JNC24_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312326.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
     gene            37291..38178
                     /gene="ugpA"
                     /locus_tag="JNC24_000801"
     CDS             37291..38178
                     /gene="ugpA"
                     /locus_tag="JNC24_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HAY9157627.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSRQSV
                     DPFGFSSQFVGLDNFVTLFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALHSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSVVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            38175..39020
                     /gene="ugpE"
                     /locus_tag="JNC24_000802"
     CDS             38175..39020
                     /gene="ugpE"
                     /locus_tag="JNC24_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HAY9157628.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            39022..40092
                     /locus_tag="JNC24_000803"
     CDS             39022..40092
                     /locus_tag="JNC24_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate import ATP-binding
                     protein UgpC"
                     /protein_id="HAY9157629.1"
                     /translation="MAGLKLQAVTKSWDGKTQVIKPLTLDVADGEFIVMVGPSGCGKS
                     TLLRMVAGLERVTEGDIWINDQRVTEMEPKDRGIAMVFQNYALYPHMSVEENMAWGLK
                     IRGMGKQQIAERVKEAARILELDGLLKRRPRELSGGQRQRVAMGRAIVRDPAVFLFDE
                     PLSNLDAKLRVQMRLELQQLHRRLKTTSLYVTHDQVEAMTLAQRVMVMNGGVAEQIGT
                     PVEVYEKPASLFVASFIGSPAMNLLTGRVNNEGTHFELDGGIVLPLNGGYRQYAGRKM
                     TLGIRPEHIALSSQAEGGVPLVMDTLEILGADNLAHGRWGEQKLVVRLAHQERPTAGS
                     TLWLHLAENQLHLFDGETGQRV"
     gene            40089..40832
                     /gene="ugpQ"
                     /locus_tag="JNC24_000804"
     CDS             40089..40832
                     /gene="ugpQ"
                     /locus_tag="JNC24_000804"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAY9157630.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQQAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QRAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(40819..41259)
                     /locus_tag="JNC24_000805"
     CDS             complement(40819..41259)
                     /locus_tag="JNC24_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HAY9157631.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            41379..>42852
                     /gene="ggt"
                     /locus_tag="JNC24_000806"
     CDS             41379..>42852
                     /gene="ggt"
                     /locus_tag="JNC24_000806"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HAY9157632.1"
                     /translation="MIKPTFLRRVAIAALLSGSCFSAAAAPPAPPVSYGVEEDVFHPV
                     RAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIR
                     SKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDK
                     YGTMPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKK
                     GDTLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERT
                     PISGDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAY
                     ADRSEYLGDPDFVKVPWQALTNKAYAKSIADQIDINKAKPSSEIRPGKLAPYESNQTT
                     HYSVVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNIYGLVGGD
                     ANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTV"
BASE COUNT        10235 a        10822 c        11746 g        10049 t
ORIGIN      
        1 caaaccatca agataagcat tcagataaag ttaacagtca tcaacaccat ggcaaggttg
       61 ataaaaaata tcgatcaaac acagttgaag ttaatatgct gaatgacttt tcgcaaatag
      121 ataacgattt aggcctgaat atcatctatt tcctgcaaca tggtcactgg agggtaaatg
      181 aacatagtct tcagatggag aaaatatggt tttacaatag tgagccatct atagatattc
      241 aagaatataa tagtttcgcc gataatacta ctaacacgtt tatctttaca attataccag
      301 acaataacca tgtgataaaa ttatcgtctc ccattacagt taagattgag tgtaaggatg
      361 gttattattt tattaatttc tccggagata aatcagacat aatttataaa tcagacataa
      421 cttataaagt taatgggctt tctatcatac ctgaaaattt ctttactctt cttagcggta
      481 attttaaagc tgactggcgt tgggatgtct ctaaagagac gtttcctaag gataagtttg
      541 atagctatgt taaatctgtg ttttctaaaa tagactttta taaacaatgt ggtgtgatta
      601 acccacaaaa tgccaacacg gcttattttg gcgatacaga tggaagagta ggcgctgtac
      661 tttatgcatt gctcgtttca gggcatatag gcatcaggga aaagggttgg agtttattat
      721 gtgaattatt aaaacacgaa aacatggctt catctgcata catacataaa aataataaat
      781 tgctctacaa tttattaaat acgagagaca tgatattgaa tgagttgcat caacatgttt
      841 ttttaaagga cgatgcgata accccttgta tcttccttgg ggatcataca ggcgatagat
      901 ttagtactat ttttggtgat aaatatatcc ttactttgct aaattctatg cgaaatatgg
      961 atggtaataa agatagccgt attaataaaa atgtcatcgt tctggctgga aatcatgaaa
     1021 tcaattttaa tggtaactat actgcacgac tagaaaatta taaactctct gccggtgata
     1081 cgtataactt aattaaaacg ctcgatgttt gtcattatga ctcagaaacg aaagttttaa
     1141 ccagtcatca cggaataata agaaatgaag aaaatgaatg ttattgtctc ggtgctttgc
     1201 aggttccatt taatcagatg aaagacccaa ttgatcctga ggaattagca aatatattta
     1261 ataaaaaaca taaacaacac atggatgatc gtcttatcca tctaattaga agcaatgctg
     1321 tagcatccac acctgtatat gaaaattatt ttaataacac tactgctttc agaccaaaac
     1381 ctgaagatat cttcaaatgt ggacagacgc tcaaaaaaac taaacagaaa tatggccatt
     1441 atggattagg ggtagatcag catcaaaaaa ttgataatta taccatgggg ctaaattcct
     1501 ggaagatagc acctaacgaa cgtggtgata aaaaaggtgt cccagggctt agttgttttc
     1561 aacctcaata aaacttcatg agtgctgtgg tatctctttt taattttgcc agtatttcat
     1621 aggttttctt aatcgattgt agaggcatgt tctgatgacg cctctacaat ctttagggac
     1681 tttctctgct atgagataag aaatttcata ttatgcttta aggctgaatg atttgtgcaa
     1741 aatataatta ttttcccatc gaacatgagg tgtatgtcat aagattaaat gttaactatg
     1801 tattcctcgc ggtaaataat atgaggtaaa agatgaaaat tggaactgtg gcaggcacta
     1861 acgacagtac aacgacaatc acaacaaatg atatggtgca agagcatgtt accaacttca
     1921 ctaaagaact gtttgggtat attgcaaatg gtataggtga tgatatatca agtattgcca
     1981 ggacaatgct cggtgaggtt gtggaaaaaa tagatgattg gcaaatagaa cggttccaac
     2041 aatctattca agatgacaaa atttcattta ccatacaaac gaatcattca gaaaaatata
     2101 gtatgctatc gggcatgcgc gctcatattc tacgtaggaa taactgttat cagtttattg
     2161 tgacaattaa tagtaaaaat tacggttgcc ctcttgataa taccgatatt aattggtgca
     2221 gcattgttta tttacttaat aatatgacag taaatgataa tgctaatgat gttgcagtta
     2281 ctgagtcgta caaacccatc tggaactgga aaatatcgca atacaatgta tttgatatta
     2341 aattcgaaac aatgataaaa ccccagttcg ctgacagaac ctatttctcc aattgttcac
     2401 ctgttgatcc cacaagtacc agaccgacct attttggcga taccgatggc tcagtcggtg
     2461 cagttttgta cgcgcttttt gcaacgggac atttgggaat catggctgaa ggagaaaatt
     2521 tccttagcca gttgcttaat atagaagatg aagttctcaa cgtattgcta agggagaact
     2581 ttaatgagca acttgacact aatgttaata taataatcag tatcctgaac agaagagaca
     2641 ttgttttaga aagtcttcag ccttatttgg ttattaataa agacgcagtc acgccatgta
     2701 cgtttctggg cgaccagact ggcgatcgat ttagtaatat ctgtggcgat aaatttatca
     2761 tcgatttatt aaaacgcata atgagtatca atgaaaacgt tcatgtatta gctggaaatc
     2821 atgaaacgaa ttgcaatggt aactatatgc agaacttcac gcgcatgaaa ccacttgatg
     2881 aggataccta ctctgggata aaagattatc ctgtatgttt ctatgacccc aaatacaaaa
     2941 taatggcaaa tcatcatggg ataacttttg atgatcagcg aaaacgttac attataggac
     3001 cgataacagt ctctagtgat gaaatgacca atgctctcga tccggtcgaa ttagcggcaa
     3061 ttatcaataa aaagcaccat gctgtaatta atggcaaaaa gtttaaaaat agccgggcta
     3121 tctcttgtcg atcctttaat cgttattttt ctgtttcgac agattacaga cctaaacttg
     3181 aagcactatt agcatgctca caaatgttag gtataaatca agttgttgca cataacggta
     3241 atggtggaag agaatgtata ggtgaaacgg ggacagtttt aggtcttaac gcccgtgaca
     3301 gtaaacatgc tggaagaatg ttcagcatgc ataattgcca aattaaccct ggtgcagggc
     3361 ctgagataac cactccctgg aagtcttacc agcatgaaaa aaacagaaat ggacttatgc
     3421 ctttgatcag aaggcgcaca atgttacagc tctgaaccat agacaacatg aatattaatg
     3481 ttactatctg gttgagactg ttgataaaac gtaaaaagga atgctctgtt cctgcaactg
     3541 aacaggcgtg aaacgctatc caacaggatg gataccgttt tgtcagccag ttttatagat
     3601 cacacttatg agtatgcgtg tttcatttac ggccaggaat gctgatgatg acaacattac
     3661 tacccgtttt cactaagcct tccccgttgg cgctcaatgc tctgcgcgct ggtcgtattt
     3721 gtcgtttcct tcttatcccg gatggaagaa tccgctgaac gtcgttcggc ggcagttgta
     3781 acacgcgtcg tttgcttgca gggattatgg cgggtcgatt atggataaga gtaagcgcca
     3841 tctggcgtgg tgggttgtcg ggttactggt ggtggctgcc gtcgtggcgt ggtggctgtt
     3901 gcgcccggcg ggtgtgccgg aaggctttgc tgtcagcaat gggcgcattg aagcgacgga
     3961 agtggatatc gccagcaaaa ttgccgggcg tatcgacacc attctggtga aagaaggcca
     4021 gtttgttcgc gaaggcgaag tgctggcgaa gatggatact cgcgtgttgc aggagcagcg
     4081 actggaagcc atcgcgcaaa tcaaagaagc acaaagtgcc gttgctgccg cgcaggcttt
     4141 gctggagcaa cggcaaagcg aaactcgtgc cgcgcagtcg ctggttaatc aacgccagtc
     4201 cgaactggac tccgtggcta aacgtcatac gcgttcccgc tcgctcgccc aacgaggggc
     4261 tatttctgcg caacagctgg atgacgatcg cgccgccgct gagagcgccc gagctgcgct
     4321 ggaatcggca aaagctcagg tatcggcttc taaagcggct atagaagcgg cccgtaccaa
     4381 tatcattcag gcccaaactc gcgtcgaagc cgcgcaagcc actgaacggc gtattgccgc
     4441 agatatcgat gacagtgaac tgaaagcccc gcgtgacgga cgcgtgcagt atcgggttgc
     4501 tgagccaggg gaagtgctgg cggcaggcgg tcgggtgctg aatatggtcg atctcagcga
     4561 cgtctatatg actttcttcc tgccaaccga acaggcgggc acgctgaaac ttggcggtga
     4621 agcccggctg atcctcgatg ccgcgccaga tctgcgtatt ccggcgacca tcagttttgt
     4681 cgccagtgtc gcccagttca cgccaaaaac cgtcgaaacc agcgatgaac ggctgaaact
     4741 gatgttccgc gtcaaagcgc gtatcccacc ggaattactc cagcagcatc tggaatatgt
     4801 caaaaccggt ttaccgggcg tagcgtgggt gcgggtgaat gaagaacttt cgtggcctga
     4861 cgacctcgtg gtgaggttgc cgcaatgacg catctggaac tggttcccgt cccgcctgtc
     4921 gcgcaactgg cgggcgtgag ccagcattat ggaaaaaacg ttgcgctgaa caatattact
     4981 ctcgatattc cggcccgctg tatggtcggg ctgattggtc cggatggcgt cgggaagtcg
     5041 agcttattgt cgttgatttc cggtgcccgc gtcattgagc agggcaatgt gatggtgctg
     5101 ggcggcgata tgcgcgaccc gaggcatcgc cgcgacgtct gcccgcgcat cgcctggatg
     5161 ccgcaggggc tgggcaaaaa cctctaccac accttgtcgg tgtatgaaaa cgtcgatttt
     5221 ttcgctcgcc tgttcggtca cgacaaagcg gagcgggaag tgcgaattaa tgagctgctg
     5281 accagcacgg ggttagcacc gtttcgcgat cgtccggcag ggaaactctc cggcgggatg
     5341 aagcaaaaac ttgggctgtg ctgcgcgtta atccacgacc cggaactgtt gatccttgat
     5401 gagccaacaa cgggggttga cccgctctcc cgctcccagt tctgggatct gatcgacagt
     5461 attcgccagc ggcagagcaa tatgagcgtg ctggtcgcca ccgcctatat ggaagaggcc
     5521 gaacgcttcg actggctggt agcgatgaat gccggagaag tgctggcaac tggcagcgcc
     5581 gaagagctac ggcagcaaac gcaaagcgct acgctggaag aagcatttat aaatctgtta
     5641 ccgcaagcgc aacgccaggc gcatcaggcg gtagtgatcc caccgtatca acctgaaaac
     5701 gcagagattg ccatcgaagc gcgcgatctg accatgcgtt ttggttcctt cgttgccgtt
     5761 gatcacgtta atttccgcat tccacgcggg gagatttttg gttttcttgg ttcgaacggc
     5821 tgcggtaaat ccaccaccat gaaaatgctc accggactgc tgcccgccag cgaaggtgag
     5881 gcgtggctgt tcgggcaacc ggttgatcca aaagatatcg atacccgccg tcgggtgggc
     5941 tatatgtcgc aggcgttttc gctctataac gaactcaccg tgcggcaaaa ccttgagtta
     6001 catgcccgtt tgtttcacat cccggaagcg gaaattcccg caagagtggc tgaaatgagc
     6061 gagcgtttta agctcaacga cgttgaagat gttctgccgg agtcattgcc gctcggcatt
     6121 cgccagcggc tttcgctggc ggtggcggtg attcatcgcc cggagatgtt aatcctcgat
     6181 gagcctactt ctggtgtcga tccggtggcg agggatatgt tctggcagtt gatggtcgat
     6241 ctctcgcgcc aggacaaagt gactatcttc atctctaccc actttatgaa cgaagcggaa
     6301 cgttgcgacc gcatctcact gatgcacgcc ggaaaagtgc ttgccagcgg tacaccgcag
     6361 gaactggttg agaaacgcgg agccgccagt ctggaagagg catttatcgc ctatttgcag
     6421 gaagcggcag ggcagagcaa cgaagccgaa gcgccgcccg tggtacacga caccacccac
     6481 gcgccgcgtc agggatttag cctgcgccgt ctgtttagct acagccgccg cgaagcgctg
     6541 gaactgcgac gcgatccagt acgttcgacg ctggcgctga tgggaacggt gatcctgatg
     6601 ctgataatgg gttacggcat cagtatggat gtggaaaacc tgcgctttgc ggtgctcgac
     6661 cgcgaccaga ccgtcagtag ccaggcgtgg acactcaacc tctccggttc ccgttacttt
     6721 atcgaacagc cgccgctcac tagttatgac gagcttgatc gtcggatgcg tgcgggcgat
     6781 atcacggtgg cgattgagat cccgcccaat ttcgggcgcg atatcgcgcg tggtacgcct
     6841 gtggaactcg gcgtctggat cgacggagcg atgcccagtc gtgccgaaac ggtaaaaggt
     6901 tacgtgcagg caatgcacca gagttggctt caggatgtgg cgagccgaca atcgacaccc
     6961 gccagccaaa gcgggctgat gaacattgag acgcgctatc gctataaccc ggacgtaaaa
     7021 agcctgccag cgattgttcc ggcggtgatc ccgcttctgc tgatgatgat cccgtcaatg
     7081 ctaagcgccc ttagcgtggt gcgggaaaaa gagctagggt cgattatcaa cctttacgtg
     7141 acccccacca cccgtagcga atttttgctt ggcaaacagc tgccgtacat cgcgctgggg
     7201 atgctgaact ttttcctgct ctgcggcctg tcggtgtttg tgtttggcgt accgcataaa
     7261 ggcagtttcc tgacgctcac cctggcggcg ctgctgtata tcatcattgc caccggaatg
     7321 gggctgctga tctccacctt tatgaaaagc cagattgccg ccattttcgg aacggcgatt
     7381 atcacgttga tcccggcgac acagttttcc gggatgatcg atccggtagc ttcgctggaa
     7441 gggcctggac gttggatcgg cgaggtttac ccgaccagtc attttctgac tatcgcccgc
     7501 ggaacgttct cgaaagcgct ggatctgact gatttgtggc aactttttat cccgttgctg
     7561 atagccatcc cgctggtgat gggcttaagc atcctgctgc tgaaaaaaca ggagggatga
     7621 tgcgccattt acgcaatatt tttaatctgg gtatcaaaga gttgcgcagt ctgctcggtg
     7681 ataaagcgat gctgacgctg attgtcttct cgtttacggt gtcggtgtat tcgtcagcga
     7741 ccgttacgcc aggatcgttg aacctcgcgc cgatcgccat tgccgatatg gatcaatcgc
     7801 agttatcgaa ccggatcgtt aacagcttct atcgtccgtg gtttttgcca ccggagatga
     7861 tcaccgccga tgagatggat gccggactgg acgccggacg ctataccttc gcgataaata
     7921 ttccgcctaa ttttcagcgt gatgtcctcg ccggacgcca gccggatatt caggtgaacg
     7981 tcgatgccac gcgcatgagc caggcattta ccggcaatgg gtatatccag aatattatca
     8041 acggtgaagt gaacagcttt gtcgcgcgct accgtgataa cagcgaaccg ttggtatcgc
     8101 tggaaacccg gatgcgcttt aacccgaacc tcgatcccgc gtggtttggc ggggtgatgg
     8161 cgatcatcaa caacattacc atgctggcga ttgtattgac cggatcggcg ctgatccgcg
     8221 agcgtgaaca cggcacggtg gaacacttac tggtgatgcc gataacgccg tttgagatca
     8281 tgatggcgaa gatctggtcg atggggctgg tggtgctggt ggtatcggga ttatcgctgg
     8341 tgctgatggc gaaaggtgta ctgggcgtac cgattgaagg ctcgatcccg ctgtttatgc
     8401 tgggcgtggc gctcagtctg tttgccacca cgtcaatcgg catttttatg gggacgatag
     8461 cgcgttcaat gccgcaactg gggctgctgg tgattctggt gctgctgccg ctgcaaatgc
     8521 tttccggtgg atccacgccg cgcgaaagta tgccgcagat ggtgcaggac attatgctga
     8581 ccatgccgac gacacacttt gttagcctcg cgcaggccat tctctaccgg ggtgccggat
     8641 tcgaaatcgt ctggccgcag ttcctgacgc tgatggcaat tggcggcgcg tttttcacca
     8701 ttgcgctgct gcgattcagg aagacgattg ggacaatggc gtaaggaagg gaaacttgac
     8761 ttcatactgt gatgtcatac tattgatgtc ataaagtgaa gtcataagga catgccatga
     8821 gaaccaaggt acaggcttta cggaagaaaa aaaaatacat tggatcaaat atttaaaact
     8881 cctgttcctc aagggatcaa gtggtctgat atagagtcac tggttaaagc attaggcgga
     8941 gaaattaagg aaggaagagg ttcgcgttgt aagttcatac taaatatgag cgttgcgtgt
     9001 ttccatcggc ctcatccgtc gccagatacc gataaaggcg ctgtagaaag cgtgcgtgac
     9061 tggttactaa gtataggggt aaaaccatga tcaaactgaa gacgccaaac tctatggaga
     9121 ttgccgggca accagctgtt attacctacg tgccagaact gaatgcattt cgtggcaaat
     9181 tcctgggctt gtcaggttat tgtgattttg tctctgacag cattcagggg ctgcaaaaag
     9241 aaggggaact ttccctgcga gagtatcttg aggattgtaa agctgcgggt attgagccgt
     9301 atgcgcgtac agaaaagatt aagaccttta cgttacgcta tccagaatcg ctgagtgaac
     9361 gccttaataa tgcggctgcg cagcaacaag tctctgttaa tacctatatt attgaaacac
     9421 tgaatgagcg tttgaatcat ttataaattt tagcggcgta cattacgcag aatatctgcc
     9481 tttacaggat ctatctatgc aactgaactt aattttataa attaagttca gttaatatct
     9541 tttctttcct cgtttcagaa ataactcaat cttccttcgg caagcactgc aaatgcccat
     9601 gccgcacgcc gcgctgggcg ataacgtcat cggcaaaatg ctgcacgtcg cccatatcac
     9661 ctttcagcac ggcgatttcc agacagtcgt cgtgattgat atgcacatgc agcgtggcga
     9721 cggagagatc gtggtgatgg tgctgggtgg agacaatgcg gctggctaag tcgcgttttt
     9781 cgtgttcata cacataggag agcaccgcga aaccttgcgt gccgtgctgc tgagtggcct
     9841 cctgggccag ggcgctacgc agaatatcgc ggatagcttc ggaacggttg ttataaccac
     9901 gacgctggct caggctgtcc agcgtctcca gtaaatcgtc atcaagcgtg atggtgactc
     9961 gttgcatttg cgttaaacct tttctgtggt gcgacggcgc acggggaatg cgggtaatac
    10021 cgcgttttgt agcacacgtc cggcgtcaga ggaaaaggtt aatttatctc ccaccacctg
    10081 ggtttcgacg atttgtccgt tgtccatcac catcacccgc tgacagaagc gttccaccag
    10141 gcgtaagtcg tgggtgatga acaggcaggc ggtgccaaac tgttgttgta gctttttcag
    10201 caggcgaatg acgcccgcct gtaacacgag atcaaggtta gaaacggctt catccagaat
    10261 cagtagtttc ggttcgaccg ccagcgcgcg agccaggcag acgcgctgga gctggccgcc
    10321 gcttaactgc ggtgggcgtt tgtcgagaac gctgtcatcg agatcgaccg ccttcagcat
    10381 ttcgctggcg cgcgccagtt gttcggattt tttcagtgat agcaggtggc gcatcggttc
    10441 acgcaggatc tcgcgcacgg ttttgcgcgg attcacggcg ctgatggagt cctgaaatac
    10501 catctgaata tcgcggcgga acgctttacg ctgggcgcga ttgagtttcg ccagcggttc
    10561 gccacgccag ctaatattcc cctgcgcggg cgattctaaa cccaccagca accgcgcgag
    10621 ggtacttttc ccgcagccgc tgcgccccag cagggcgacg gtttcgccgc ttttcagggt
    10681 cagggaaacg ttattcagca ccgcctgatg ttgatgtttt ccgttaaatc caccgtgggc
    10741 atagtgatgg gaaaggccgg agacgttaag taaagtcatg atgccagctc cataccgtag
    10801 agggcgagat gagcggaaac caggctgcgc gttaccgcat gtttgggggc gttaaacagc
    10861 gtttctacat cgccctgttc gacaatttta ccttgtgaca tcaccgccac gtcatccgcc
    10921 agacgcgcca caacgcccat atcatgggtg accagcagca ttcccggcgc ttgtttttgc
    10981 ataatgcttt ccagcagatc gaggatgcgc gcctgtgcta ccacgtcgag gtcggtggtc
    11041 ggttcatcgg cgatgataaa cggtgattca cacagcaccg ccatcgcaat catcatgcgc
    11101 tgcaacatgc cgccgctcat ctcgaacggg tacagcttca gcacgcgcgc ggcgttttcc
    11161 agccccaccg cttctatggc agcggtaagc gtagcgtcat cggcgggttt ccctaacgcc
    11221 aggcaggttt cccgcgcgtg ggtgtgcatg gtgtgtagcg gattaaaggc gctgcgcggg
    11281 ttctgcatga tggtggcaat tttgatgccg cgcagggcgc agggataaac cgctttgcca
    11341 tcggctaaaa tttcccccgc cgtctggcga acgccagcgg gcaaaatgcc cagcgtcgcg
    11401 gcgcaggtta atgatttccc gctgccgcta ccgccgacta acgccagcac gcgcccgcgt
    11461 tgcagggtta acgatacacc gtgtaccagc ggctgcgcgg cctgtagcgc gatattacgt
    11521 agttcaatct gttgcggcat tagtgtgcgt gctccgtcac cagatgagga tccagatgat
    11581 cgcgcagtgc gtcacccacc aggttaaagg ccatcacgct gataaacagc gccagccccg
    11641 gccagaacat ttgcagcggc tgggtccaga tatactggcg cgcgtcgtta atcatcacgc
    11701 cccattcggc ggtcggcgcg gtcacaccga ggccaaggaa agacatcccc gcgacgtgca
    11761 gcatcatatg gccgatatcc agcgttgcca gcaccagcag cgaagggatc accgcgcctg
    11821 ccagatgatc gacaaacacc cgcacatggc ccgcaccgga aagccgtgac gccagcacaa
    11881 actcgcgttg gcgtagtgaa atcaccaagc tgcgcaccat gcgtgcatac cacgcccagt
    11941 gcgacagggc gatggcgata attacgttgg tcagcccggt gccgagcacg ccaaccataa
    12001 agaacgacag aatcgaggtc gggaaggtca taaacatatc ggcgacgcgc atggtggcct
    12061 gatcaacgcg cccgccaatc aacccggcgc tgccgccaat aaccagccct aatgtcagca
    12121 ccagcagcag gcaggccatt accgaaccga gcgacacgcg ggtcgctgcc atcagccgcg
    12181 agaaaatatc gcgacctaag tgatcggtgc ccagccagtg ctgcgcatcc ggcgaaagca
    12241 ggcgcgacgg caaatcaatc gcctgggggt catacggcag ccaccactgg ctggtgagcg
    12301 caatcagcgc cagcagggcg ataatgatca gcgccaggcg taccgaccag cgggaagaga
    12361 ggaaaaagtt cacgcgtgcg ctccttcatg acgacgaatg cgcgggtcca gcgcggcgtt
    12421 gagcaaatcg acaatcaaat tacagaccac aaaaaccacc accatcatca gcgtaaagca
    12481 ctgaatcacc ggatagtcac ggttaaaaat cgccgacacc gcatagcgcc cgacgcccgg
    12541 ccaggcaaag atgttttcga taatcatcgt cccgccaatc agttcgccga tgtgcatccc
    12601 cacggcggtg atcatcggca gcgaggcatt gcgcagaata tggcggcgtt cggtctgttt
    12661 gtcgttcagg ccgcgcagac gcgcccaagt gacgtgacgc tgaccggcga cgtccagcat
    12721 actggcgcgc agcagtcgcg cgttaatcgc cagcgacata aaggcaatgg aaaccgcagg
    12781 caaaatgatg tgctgccagc cgccgtaacc catcgcgggt agccattgca gatacaccga
    12841 aaacgccatc accagtaaaa acgccagcca gaagttaggc atcgacacgc caagaaacgc
    12901 gatgaaacgt acggcgaaat ccggcagacg gtcgcgatgg cgcgccgccc agataccgag
    12961 cggtacggaa gtgagcagaa ttaataccag cgccgcacct gcaagttcca gcgtggcggg
    13021 caggaagttc agcatatcgt ccagcaccgg gcgttggctg gcgaatgaga taccaaagtc
    13081 aagatgcagc gccttccaca accaggtacc gtactggacg tacagcggct gatccagccc
    13141 gagcatggtg cgggtagagg ccagcatctc cggcgtcggc ggcaggttag ataaacgcag
    13201 ataatcgagc gccgggtcgc cggtaccgag gcgcagcatc agaaaaatga tcaccgaggc
    13261 ggcgagcacc atcgggatca gcagcagaaa gcggcgtaat acgtaacgca acattaaggt
    13321 ttcaccggtt taatctgttc gaacggaatt tcggtggcga tcggcgcgta ggggatatta
    13381 cccagctccg gttttgatac caccatcatc gagatgtagc tgataggcag ataaaccgcc
    13441 tcgtcatgca gacgggtcaa aatgtcgcga tacagcgcct gacgttgcgt ttcgtcatgg
    13501 gtcgccagca cttcgccgat ctctttatca atcagcggct tgtcggctaa tccttgctgt
    13561 gcctggaagt cagcgtgcga cggtacgcgc attgaactca ggaaggcgtg tggatcgtat
    13621 ggcgcgcccc aggtgcggtg gaaaatcatg ccaaaacgac cgtcgcgctg acgagcatag
    13681 atactgctct cttcttcgcc aatcagcgag acatctgcgc caatctggcg catatcagcc
    13741 tgaatgattt ccgccatcga tttgcttaac gcatcggtgc cgatgaacga cagttcaatg
    13801 cgcagcggct gaccattttt ctcgcggatg tctttgcccg caggcagcgt ccaaccggct
    13861 ttttccagca actctttcgc tttctgcgga tcgtactggc gcggtttcag gccgagatta
    13921 gcgtaaggca cagaaggggc aaacagggtg tcggcgacct gcggggtgcc atataacgcg
    13981 ttatcaatca gcgatttttt gtttaccgcg taattaagag cttcacgtac tgccagctcg
    14041 ttggtggggg ctttggcggt attgagcgcc agcatcacgg tttcgatcgg ctgtgacagt
    14101 tgggtgtggt aagccggatt ctggctaaag cgggcgaagg tatcgagcgg taataaccct
    14161 tcgtttccgt acagcaggtc gatatcgcca gtttcaaacg ccaccgcgcg ggtagtcggg
    14221 tccgggatga cgttaaaggt gatcttttta atcgctggct tttcgcccca gtagttttcg
    14281 ttacggacga agacatcgta ctgattcagt ttcgattcct gcaaaatcca cggtccggtg
    14341 ccaatcggcg ctttaattcc gttcatggtt tcatggtttt taaactgcga gggagcgata
    14401 aaacggaaag gacggggcag ggccagttct tgcaggaaag gatagtaggc gcttttcagg
    14461 gtaatttgca gctctgtttt actgagtgct ttaacatcaa caatctggtt tgccagctcc
    14521 agccaggcgt gacgttgacg gttatcgagc actgcgcgga agttttctgc cgccgcctcg
    14581 gcatcgaacg gttcaccgtt ggagaatttc acgtcatcac gcagggtgaa ggtccaggtt
    14641 ttaccatctt ctgaatgagt ccagcttttt gccagccacg ggatcaccga accgtctgcc
    14701 tgatatttca ccaatggttc ataaaccatg ctctgggcga acatctggtt aggcgtgtaa
    14761 aggtgcgggt ttagtggccc gacattcacc ggccaggcgg tagtgatttc atctggtgca
    14821 gcggcatgga cgataaaaga cgcacaagcc agcagcgcaa atagagtgcg gcggagtgtg
    14881 gagagcatgg taaccccaat ggattaaaat agatggcgga aataagtatg acgattttaa
    14941 gtattcgtca tactgattac ctgttctgga tcaatagatg ggcagtcggg tgggcggatc
    15001 agttaactga atcgatccat tgcacactgt cggcggtgag cgtaaagggg gtgggggtgc
    15061 agatcgcaag gcttaaattt tctagctgac aatggctgac tgacagcgag gagtgatagg
    15121 tgctatctac gctgacgatt tgccaggcgc tgccgccacg ctgtttaacg atggcttctt
    15181 tgcgcgtcca gatgcgccag aacatttcca gttgctgatc aggatgcacg gcgtccattt
    15241 cagcgtgttc cccgaggctg aacacggcgt tcgccagcca gcgccagttg gcgcgcgggc
    15301 gaatcacttc gatatcgcag ccgacttcgc cttcatcgct caacagcaga gcgatatcgt
    15361 caccgctatg gcttaagttg aaccatagcg gcgtttccgg cgcgaatgca ggtttgcctt
    15421 gttcgccata gatgatctcc ggtagcgggg aaagcgtgtg cgaaagcaat gcacgccccg
    15481 ccagccagcg ttcgcgtcgt ggaccttgcg gtgcttgctc gcgtaaaccc ggtggcagtg
    15541 gagctgcgct taaggtcgaa actttcccca gaactatccg atacatatca gggccaacgt
    15601 ttaatggaaa atgaaagtgc gtatcgtatc acttgtcgcc tcatcccggt aaccgacttt
    15661 tcggtctgcc tggccccagt aaaatcgcca gtttgctacc gcctttggtg gtttccatcc
    15721 agattttaca cacactggtt aatggcaccg aaagtagcat ccctaccggg ccgagcagcc
    15781 atccccaaat taacaatgaa agaaatacca ccatggtgga catccccagg cgatggccca
    15841 tcatccgtgg ttctaaaata ttgccgatga ccatatggac cactaaaaac aatgcgccga
    15901 ccagaataca ttcgtaaaca ccattaaaca gcagcacctg aatcattggc ggtacggcgg
    15961 aaattaccgc gccgatattg ggcacgtagt tgagcaaaaa cgccagtact gcccacatca
    16021 gcgcaaactg cacccccatc agctccagcc ccagccagac gatgacacct gtccataaac
    16081 tgagtagcgt cttcaatgca agatagtgcg aaacgccttt aagtgcgcgg tgtaatcctg
    16141 cgatgtgaat ctgtgggtta ttcagcgcaa aacgcatttt gtaagggacg tggcgcactt
    16201 caaacagcat aaaaactacg gtcatcacca gcaaaagcac gctcgccatt gccccggaaa
    16261 gcccggtcat tagcgctgtg gtgaaggtaa ccactttttc cgagtccatc cgctgcagca
    16321 ttcgctccgg cgacatatgc aaattaagaa aaggcaacat ctcctgcaat ttaaaaagtt
    16381 tgcgcgtcag ctccttatta aacttcggca gcatagagat aaattcgtta aacgatgccg
    16441 ccagtacgcc gaccagcgcg gttagtgcga tcagcatcac caccactaca atcgtaatgg
    16501 caacggggcg ttgtactccc cgacgaataa accaggtaac gagcgggttg aggacgatgg
    16561 caaaaaacag cgccagtaac agctgcacaa tgatatctgc cgctgcgtga atgcccgcga
    16621 ggatcactac cagcgaggcc agcttgagca gaatgtgcat gcccgtttta tcgggttgag
    16681 ggatttccat tggggcttcc ttgtgacttt ttgtattaag tgtagcggga gtcacgccag
    16741 caatattctg attcttactg ctgattttca cgtcagcacg tggtaaaaat gaaacactgt
    16801 tgtaaaaatg tggtgatcct catgcccgaa cccgtagccg aacccgcgct aaacggattg
    16861 cgcctgaatt tgcgcattgt ctccattgtc atgtttaact tcgccagcta cctcaccatc
    16921 gggttgccgc tcgctgtatt accgggctat gtccatgatg tgatgggctt tagcgctttc
    16981 tgggcaggat tggttatcag cctgcaatat ttcgccacct tgctgagccg tcctcatgcc
    17041 ggacgttacg ccgatttgct gggccccaaa aagattgtcg tcttcggttt atgcggctgc
    17101 tttttgagcg gtctgggata tctgacggca ggattaaccg ccagtctgcc cgtcatcagc
    17161 ctgttattac tttgcctggg acgcgtgatc cttgggattg ggcaaagttt tgccggaacg
    17221 ggatcgaccc tgtggggcgt tggcgtggtt ggctcgctgc atatcgggcg agtgatttcg
    17281 tggaacggca ttgtcaatta cggggcgatg gcgatgggtg cgccgttagg cgtcgtgttt
    17341 tatcactggg gcggcttgca ggcgttagcg ttaatcatta tgggcgtggc gctggtggcc
    17401 attttgttgg cgatcccgcg tccgacggta aaagccagta aaggcaaacc actgccgttt
    17461 cgcgcggtgc ttgggcgcgt ctggctgtac ggtatggcac tggcactggc ttctgccgga
    17521 tttggcgtca tcgccacctt tatcacgctg ttttatgacg ctaaaggttg ggacggtgcg
    17581 gctttcgcgc tgacgctgtt tagctgtgcg tttgtcggta cgcgtttgtt attccctaac
    17641 ggcattaatc gtatcggcgg cttaaacgtg gcgatgattt gctttagcgt tgagataatc
    17701 ggcctgctac tggttggcgt ggcgactatg ccgtggatgg cgaaagtcgg cgtcttactg
    17761 gcgggggcag ggttttcgct ggtgttcccg gcattgggcg tagtggcggt aaaagcggtt
    17821 ccgcagcaaa atcagggggc ggcgctggca acttacaccg tatttatgga tttatcgctt
    17881 ggcgtgaccg gaccactggc tgggctggtg atgagctggg cgggcgtacc ggtgatttat
    17941 ctggcggcgg cgggactggt cgcaatcgcg ttattactga cgtggcgatt aaaaaaacgg
    18001 cctccggaac acgtccctga ggccgcctca tcatcttaaa acttactgaa taaccagcgt
    18061 attaatgatg ttttctgcgg tggtctgcgc tttttgctga tcgtcagctg gcagagtaat
    18121 ttgcatggtc agcagttgat tacccacgtt acccagaata acggaagagt acgccgtctg
    18181 gcctttcgcg gagataatac tgtctaactg ctgcattttg tgacctttca gctcaatggc
    18241 tttgttggtt accacttgca gctgcggatc gcggctacgt tgctgatctt ccagacgctt
    18301 cgccaacacc gccagatctt ctttcggatc atcgcccatg atgacgatga ctgctttctg
    18361 cccggtggcg tcggaccaga catgcatgtt attggcctgc gttcccagct taccgctctg
    18421 gtcggtcata tccgctggca gcgagaaact taacttgcca tcaagcaggt tgacgggatt
    18481 cccggtagcg ttactttccg cgaccgaacc ctgcgccgta gcgttagtgt ctttatcatc
    18541 acaggccgca agccccataa ccagcaggcc aattccgaca tatttaacca gattgcgcat
    18601 tgacttcttc ctttcgataa acggccataa cggctcattc atccatctta tcacaactct
    18661 gataacgaac ctttaactcg cctgcaaagc gttgatttcg gatttatctg ccagtctttt
    18721 caacagaata ttgagtaata cgccatacat tggcaggaag aaaacgatac tgattaacac
    18781 tttgaaacag taatcgacca gcgcgatttc catccagtgt tcagccataa aggcatccgg
    18841 gctacgccag aaggcaatga agaaaaaggc cagcgtgtcg ctgacgttac cgaacagtgt
    18901 ggacgctgtc ggtgccagcc accagcggcg actctgacgc aggcggttaa aaacgtgcac
    18961 gtcgaggatc tgtcccagcg cgtaggccat gaaactggcg gtggcgatac gggcgacaaa
    19021 caggttgaag tgggcgagtg cgccgaatcc ctgccaggaa cccatataga atagcgacga
    19081 gatgacgtag gagatcagca acgccgggat catcaccgca aagatgatac gacgggccag
    19141 cggtgcgcca aaaatacgca cggtcaggtc ggtagcaagg aaaataaacg ggaagctaaa
    19201 cgcgccccag gtggtatgga aacccaaaat ggagacggga agctgaacca gatagttact
    19261 ggaggtgatc accagcagat gaaataacga taaccagaac aacgccttat agcgttgagt
    19321 ttgcgagaaa acgttcatat tgtacctttt tgattaacca ttggggtgag ggaacccaat
    19381 acgtacgaca cgtctgttac cttaacataa taacgtgctt accctttttt cgagccgccg
    19441 catgatactg ctttacgttg acaatgcaat ggttaatttt cacgcaatcg ttaacctggt
    19501 ttgcttacgg actcacaggg cgtaaactag cgccgttttt ttatgtgatg agaagaaaat
    19561 gaccgatctc ttttccagcc ctgaccacac actcgacgca cttggcctgc gctgcccgga
    19621 acccgtaatg atggtgcgca aaactgtgcg caatatgcag cctggcgaaa cgttgctgat
    19681 tatcgccgac gatccggcca ctacccgcga tattcctggg ttttgtacct ttatggaaca
    19741 cgaactggtt gcgaaagaga cggatggact gccttatcgt tatttgattc gtaaaggcgg
    19801 ttgatgggga aagattggct tcgatgccgc cttttcccct caccctaacc ctctccccag
    19861 aggggcgagg ggaccgatcg cgctcaatgt tgcgatcggt ttgccttatc tcctgcgcaa
    19921 caatcttaac gcattcgctg tcaccagcac cgtcgcaccc gtatctgcca gcaccgccag
    19981 ccacaggccg gtcatcccta acagcgtggt gacgaggaag atccctttca gccccagcgc
    20041 aatggtgatg ttctggcgga tattggcgtg agtggcgcgt gccagttcaa tcatttgcac
    20101 caggccgcgc aggtggttat gggttaatgc tgcatcagcg gtttccagcg ccacgtctgt
    20161 gccactgccc attgcaatcc caatggcggc agctttcatc gctggcgcgt cgttaatacc
    20221 atcaccgacc atcgccaggg gcgcatgttg attcagcttg gtcaccgctt tgactttatc
    20281 ttccggcaat aggcccgctt taaactccag ccccagctcc ccggcgattg ccgccgctgc
    20341 acgtggatta tcgccggtga ggatcactcc tttgaccccc agcgcgttca gttcactgat
    20401 ggcagttgca gcatcggcgc gcagggtatc ctgcaatgca atgataccca gcacgtcatc
    20461 attacgtact accagcacaa ccgtttgccc ggcgctttcc agttcattaa tcaaaccagc
    20521 aaatgcatca gcgggatgtt tcccggcagc gcaaatcaat acgcgctcac cgttaacctg
    20581 cgcttcaatg ccagacccga ccagcgcccg ctgtgattcg gcggtgggaa tggcgagttc
    20641 agcaacctgt gcttcgcgta cgatggcttg cgccagtgga tgcgtcgcgc cttgctcgac
    20701 cgccgccgcc agtgtcagca gttcagattc actaataccc gttgccggat gaatcgcggt
    20761 aacgcgcggt ttaccgacgg tcagcgtacc ggttttatca aacgccacct gagtaacacg
    20821 acctagttgt tccagcgccg ctccgccttt aatcaacgcc ccacgacgcg ctgccgccgc
    20881 cagcccggag gtaatcgccg caggcgttga gataactaac gcacacgggc agccaatcag
    20941 cagcagcgtc agccctttat aaatccactc ctgccagctg gcggcaaaca gcagcggtgg
    21001 caccagcgtc accagcagag cgacggccat aatcgcgggc gtatagatac ggctgaaacg
    21061 gtcgataaac cgctcaatgg gggcgcgacg ctcttcggct tcttcgatca gtttcagaat
    21121 ccggtcaatg gcgctggcac ccggttctga cagcacttcc agcgttacca gacggtctac
    21181 gctggtggct cctgcaggaa ctttatcgcc cgtcgcgcgc tccaccggaa tggattctcc
    21241 ggtcagggcg ctttcatcaa aactggcaaa cggtgagagc agtttaccgt cggcaggcaa
    21301 acgcccacct gcggcgactt caatcacatc gccaggccgc aggctgttaa tcgccacctc
    21361 ttcccgctca ccgttacgca ggcgcgtggc ggtttctggt ttcagcgcca ttaacgcgct
    21421 aacgccctga cgcgcgcggc tggcggccca gccttccagt cgttcaccaa tcaaaaacag
    21481 cagcaacacc atcgcagctt cagccgttgc gccaataaac agtgcaccaa tagcggctac
    21541 gctcattaag gtttcaatgg cgaagtagct gccggatttg atcagccgta atgcctgacg
    21601 agcaatcggg tacagcccaa ccagcgtggt cgcgataaac gccagttgcc cgaacggatg
    21661 attgaactgc tccagacccc agctgattgc catcatcacg attagtgtaa tcagcggcag
    21721 attctctttc aggcgcgatg cttgtggctc gtcggcggcc tgctcatcgc gcagggaata
    21781 gcccgctttt tgcaccgcag attcaacttg tgcacgaatg tcattgtcgg catcaaccac
    21841 cagtttttcg gtggcgaaca acacctgcac ctgattcaca cctgcaagct ggcgcacggc
    21901 attttctacc ttgcgcgcac aggcggcgca gtccatgcca ctgactttcc agctatagcg
    21961 ggtgccggag acgttttcag agagcgttgg cgagctggag catgcgccgt cgcagcaaca
    22021 gtcgttggcg ttctgtaccg tggttagcgg tttgaacgca gcaaattgag gggctttctt
    22081 gccgtgattg tcaggtgtcg acatggcata ctccggttaa gttttttctc attaaccgaa
    22141 ggatacactc tggagtcgac tccagagtca agttttatca gagatacagt gagcggatga
    22201 tgaggaagtg accggcaaag tagcaggccg cagcaatcgc gttatccgca cggaagcgtc
    22261 ggcgatagtg gctccccagc cagacaaagt tactgataaa cagcaacgaa gcgccaacaa
    22321 acgcagagag cgccggagcg gtcggacgga agaaccacag ttcacctgcc agccacacca
    22381 tcaccagcgt catgccgata aacgtgcaga taggccaacg atactcttcc agccgcgtcc
    22441 agataatcgc cagtaacagc gcacccagaa ccagcaacac cagtggcaga ggccagaaga
    22501 aagagagcgt catctgactg gcgaaataga tggtgtacag caggtgcgag aggaaaaacg
    22561 cgccgatggc gtacatcaga cgttgacgtg gcaacagggt tagcgcatcg cccagcagtg
    22621 aggcgcacag ccctgccagc accagatagc taatggcgtc gaacatcggc gcttgccagg
    22681 ccagtaacag caggagaaga agggttaacg gtttaaacac ccagcgttgc caggttggcc
    22741 cacgatacga tgcatccaca gatagccatg cggaaagaca gacagcgata aacgaccaaa
    22801 gcatcttagt tccttgaatt cgttattttt tcgtaagcgg aatgcctacg gtcttgcttc
    22861 cagtttagtg gcgagggtgg acggatgaca acaccataac tggcagggta tgcttatttc
    22921 cgcattttcc gatgagggat aaagatgagc aaaccacctc ttttctttat tgttatcatt
    22981 ggcttaattg tcgtcgccgc atcgtttcgt tttatgcaac agcgacggga aaaagctgat
    23041 aatgatatgg ctccgctcca gcaaaagctg gtggtggtga gcaacaagcg ggaaaaaccg
    23101 attaacgatc gccgttcgcg ccagcaggaa gtgactccgg caggcaccag tatgcgctac
    23161 gaggcaagct tcaaaccgca aagcggagga atggagcaga cgtttcgcct cgacgcccag
    23221 cagtaccacg ccctgacagt gggcgataaa ggtacgctga gctataaagg aacgcgcttt
    23281 gttagctttg ttggcgaaca ataaggttat tttttaactt taaatttctt ctgccagacc
    23341 agcagttcaa acacgccgaa aaggaaaatc cgtacctttt cggcggtggt catctgcggg
    23401 ccatctttcg gtaaagtgga tttcaatagc gccagctgca taccatgcat cagcacggta
    23461 aaaatcagcg ccacgttaac gaaaatattt agcgggcgcg ggaagggatg caccagattg
    23521 aggattaaaa atccccaaac gcagagcatt aacaaacgac caatattaat cagcatcact
    23581 ttctccttgt gcttcgcgtt gatacagccg ataagccacc tgacccgcca ctttttcccg
    23641 atgtaacgac cagtttgccg gaacggtggg cagaccgttt tcgacttcgc tttcgacata
    23701 aatcagggct tcgtcagcca gccagccgtt atcttccagt aaatttatcg tctcttctaa
    23761 caagccacgg cggaacggtg gatcgacaaa cacgatatta tgcggtgtgc ctttttgcgc
    23821 caggaatgac atcgcgttgc tgttcaccac gcgtgcattg cctgctttta gtgtcgccag
    23881 attcttaatt aactgctgag aaactgcacg atccatctca attaacgtgg cccccgcagc
    23941 gtagcgcgat aacgcttcca gccccagcgc gccgctcccg gcgaagcaat ccagacattg
    24001 ggcgtcaaca atgaccggag ccagccagtt aaacaacgtt tcgcgtacgc ggtcggtggt
    24061 ggggcgcaga cctgggctat caggaaccgg gagtttacgg cctcgccact gcccgccaat
    24121 aatgcggatt tggccgctgc cggaatgatt cggttttttc atgataaatt gctcaatccg
    24181 ccagataaca catacttgca ggcggtgatg tgaattaagt taagtgatag actatttcat
    24241 cattttttta gctgctatgt acatagcgtt aacgctgtgc catgaagcaa cagcgaggag
    24301 tgtagtcgca aatggcgaaa gaaaaaaaac gtggcttttt ttcctggctg ggctttggtc
    24361 aaaaagagca gaccccggaa aaagagacag aagttcagaa tgaacaaccg gttgtagaag
    24421 aaatcgttca ggcgcaagag cctgtgaagg cttcggaaca agccgttgaa gagcagccgc
    24481 aggcgcatac tgaagccgag gcggaaactt ttgctgccga cgttgtggaa gtcactgaac
    24541 aggttgttga aagtgaaaaa gcgcagcctg aagcggaagt tgttgcacag ccggaaccgg
    24601 tcgtagaaga aacgccggag ccagtggcta tcgaacgtga agagctgccg ttgccggaag
    24661 acgtcaacgc cgaagaggtt tcgccagaag agtggcaggc tgaagcggaa accgtagaga
    24721 ttgtcaaagc ggcggaagaa gaagcggcta aagaagaaat caccgacgaa gagctggaag
    24781 cacaggcgct ggcagccgaa gcagcagaag aggcggtgat ggtggttcct ccggcagaag
    24841 aagagcagcc ggtggaagaa atcgctcagg agcaggaaaa accaaccaaa gaaggtttct
    24901 tcgcgcgcct gaaacgcagc ctgttaaaaa ccaaagaaaa tctcggttcc ggatttatca
    24961 gcctgttccg cggtaaaaaa atcgacgatg atctgtttga ggagctggaa gagcagcttt
    25021 tgatcgccga tgtgggcgtg gaaaccacac gtaaaattat caccaatctg acggaaggcg
    25081 catcccgcaa gcagcttcgt gatgccgagg cgctctatgg cctgctgaaa gaagagatgg
    25141 gtgagattct ggcgaaagtc gatgagccgc tgaatgttga aggtaaaacg ccgttcgtga
    25201 tcctgatggt gggcgtcaac ggtgtgggta aaaccacgac gattggtaag ctggcacgtc
    25261 agtttgagca gcagggtaaa tcggtgatgc tggcggcggg cgatactttc cgtgcagcag
    25321 cggttgaaca gcttcaggtc tggggtcagc gcaacaatat tccggtgatt gcccagcata
    25381 ccggggcgga ttccgcctct gttatcttcg acgccattca ggcagctaaa gcgcgtaata
    25441 tcgacgtcct gattgccgat accgccggac gcctgcagaa caaatcgcac ctgatggaag
    25501 agttgaagaa aatcgtccgc gtgatgaaga aactcgacgt tgaagcgccg catgaagtta
    25561 tgctgactat tgatgccagc accgggcaga acgcggtaag ccaggccaaa ctgttccacg
    25621 aagctgttgg cttaaccggc atcacgctaa cgaaactgga cggcacggcg aaaggcgggg
    25681 taattttctc ggtggctgac cagtttggta tccctatccg ctacattggt gtcggcgaac
    25741 gtattgagga tttgcgtccg tttaaggcgg acgactttat agaggcactt tttgcccgag
    25801 aggattaaca atgattcgct ttgaacatgt cagcaaggct tatctcggtg ggagacaggc
    25861 gctgcagggc gttacgttcc atatgcagcc gggtgagatg gcgtttctga ccggtcattc
    25921 cggcgcaggg aaaagtaccc tcctgaagct gatctgtggg attgagcggc ccagcgccgg
    25981 gaaaatctgg tttagcggcc atgacatcac gcgtctgaaa aaccgtgaag ttccgtttct
    26041 gcgccgccag attggcatga ttttccagga tcaccatcta ctgatggacc gtactgtcta
    26101 cgataacgtg gcgatcccgc tgattatcgc tggtgccagc ggtgacgata ttcgtcgccg
    26161 ggtgtcggcg gcactggata aagtcgggct actggacaaa gcgaagaact tccctattca
    26221 gctttccggc ggtgaacaac agcgtgttgg cattgcccgc gcggtggtga ataagcccgc
    26281 ggtactgctg gcggacgaac cgaccggtaa cctggacgac gcgttgtcgg aagggattct
    26341 acgtctgttt gaagagttta accgcgttgg ggtaaccgta ttgatggcaa cgcacgacat
    26401 caacctgatt tcacggcgtt cccatcgcat gctcaccctg agcgatggtc acttgcatgg
    26461 aggcgtgggc catgaataag cgcgatgcaa tcaatcatat tcggcagttt ggtgggcgtc
    26521 tggatcgctt ccgtaaatcg gtcggcggct caggcgacgg cggtcgtaac gcaccgaaac
    26581 gcgcgaaatc ctcgccaaaa ccggtaaatc gcaaaaccaa cgttttcaac gaacaggtgc
    26641 gctatgcctt ccacggcgca ttgcaggatc tgaaaagcaa accgttcgcc acgtttttaa
    26701 cggtgatggt tatcgccatc tccctgacgc tgcccagcgt ctgttatatg gtgtacaaaa
    26761 acgttaatca ggcggcgacg cagtattatc cgtcaccgca aatcactgtt tacttgcaaa
    26821 aaacgctgga cgatgacgcc gctgcgggcg tggtggcaca gttgcaggcc gagcaaggcg
    26881 tggagaaagt gaactatctt tctcgtgaag acgcactggg cgagttccgt aactggtctg
    26941 gttttggtgg tgcgctggat atgctggaag aaaacccgct tccggcagtg gcggtggtga
    27001 tcccgaaact cgattttcag gggacggaat cactcaatac gctgcgtgat cgtatcacgc
    27061 agattaacgg cattgacgaa gtgcggatgg atgacagctg gtttgcccgt ctggcggcgt
    27121 tgaccgggct ggtcgggcgc gtttcggcga tgatcggcgt gttgatggtg gcggcagtgt
    27181 tcctcgtcat cggcaacagc gtacgtctga gcatctttgc tcgccgtgac tccatcaacg
    27241 tgcagaaact gattggtgcg acggatggtt tcatccttcg cccgttcctg tatggtggcg
    27301 cactgctggg attttctggc gcattgttgt cattaatttt gtcagaaatt ctggtgctgc
    27361 gattgtcatc ggcggttgcg gaagtggcac aggttttcgg aacgaagttt gatatcaatg
    27421 atttatcgtt cgatgaatgc ctgctattgc tgctggtatg ctcgatgatt ggctgggtgg
    27481 cagcgtggct tgccacggta caacatttac gccactttac gcctgaataa taaaagcgtg
    27541 ttatactctt tccctgcaat gggttccgta gcagggaaag agatcccgtt gtctcttccc
    27601 ggtatttcat ctctatgtca cattttgtgc gtaatttatt cacaagcttg cattgaactt
    27661 gtggataaaa tcactgtctg ataaaacagt ggatgataac ctcgttgctc ttaagctctg
    27721 gcacagttgt tgctaccact gaagcgccag aagatatcga ttgagaggat ttgaatgact
    27781 gacaaaatgc aaagtttagc tttagcccca gttggcaacc tggattccta catccgggca
    27841 gctaacgcgt ggccgatgtt gtcggctgac gaggagcggg cgctggctga aaagctgcat
    27901 taccatggcg atctggaagc agctaaaacg ctgatcctgt ctcacctgcg gtttgttgtt
    27961 catattgctc gtaattatgc gggctatggc ctgccacagg cggatttgat tcaggaaggt
    28021 aacatcggcc tgatgaaagc agtgcgccgt ttcaacccgg aagtgggtgt gcgcctggtc
    28081 tccttcgccg ttcactggat caaagcagag atccacgaat acgttctgcg taactggcgt
    28141 atcgtcaaag ttgcgaccac caaagcgcag cgcaaactgt tcttcaacct gcgtaaaacc
    28201 aagcagcgtc tgggctggtt taaccaggat gaagtcgaaa tggtggcccg tgaactgggc
    28261 gtaaccagca aagacgttcg tgagatggaa tcacgtatgg cggcacagga catgaccttt
    28321 gacctgtctt ccgacgacga ttccgacagc cagccgatgg caccggtgct ctatctgcag
    28381 gataaatcat ctaactttgc cgacggcatc gaagatgata actgggaaga gcaggcggca
    28441 aaccgtctga ccgacgcgat gcaaggtctg gacgaacgca gccaggacat catccgcgcg
    28501 cgctggctgg acgaagacaa caagtccacg ttgcaggaac tggctgaccg ttacggtgtt
    28561 tccgctgaac gtgtacgcca gctggaaaag aacgcgatga aaaaactgcg cgctgctata
    28621 gaagcgtaat ttccgctatt aagcagagaa ccctggatgc gagtccgggg tttttgtttt
    28681 ttgggcctct acaataatca atttcccctc cggcaaaacg ccaatcccca cgcagattgt
    28741 taataaactg tcaaaatagc tattccaata tcataaaaat cgggtatgtt ttagcagagt
    28801 atgctgctaa agcacgggta gccatgcata aaacgaaata aagtgctgaa caacaacatc
    28861 acaacacacg taataaccag aagaatgggg attctcagga tgaacataaa gggtaaagcg
    28921 ttactggcag gatgtatcgc gctggcattc agcaatatgg ctctggcaga agatattaaa
    28981 gtcgcggtcg tgggcgcaat gtccggtccg gttgcgcagt acggtgacca agagtttacc
    29041 ggcgcagagc aggcggttgc ggatatcaac gctaaaggcg gcattaaagg caacaaactg
    29101 caaatcgtaa aatatgacga tgcctgtgac ccgaaacagg cggttgcggt ggcgaacaaa
    29161 gtcgttaacg acggcattaa atatgtgatt ggtcacctct gttcttcatc aacgcagcct
    29221 gcgtctgaca tctacgaaga cgaaggcatt ttaatgatca ccccagcggc aaccgcgccg
    29281 gagctgaccg cccgtggcta tcagctgatc ctgcgcacca ccggcctgga ctccgaccag
    29341 gggccgacgg cggcgaaata tattcttgag aaagtgaaac cgcagcgtat tgctatcgtt
    29401 cacgacaaac agcaatacgg cgaaggtctg gcacgagcgg tgcaggacgg cctgaagaaa
    29461 ggcaatgcta acgtggtgtt ctttgatggt atcaccgccg gggaaaaaga tttctctacg
    29521 ctggtggcgc gtctgaagaa agagaatatc gacttcgttt actacggcgg ttaccacccg
    29581 gaaatggggc aaatcctgcg tcaggcacgc gcggcagggc tgaaaactca gtttatgggg
    29641 ccggaaggtg tggctaacgt ttcgctgtct aacattgcgg gcgaatcagc ggaagggctg
    29701 ctggtgacca agccgaagaa ctacgatcag gttccggcaa acaaacccat tgttgacgcg
    29761 atcaaagcga aaaaacagga cccaagcggc gcattcgttt ggaccaccta cgccgcgctg
    29821 caatctttgc aggcgggcct gaatcagtct gacgatccgg ctgaaatcgc caaatacctg
    29881 aaagcgaact ccgtggatac cgtaatggga ccgctgacgt gggatgagaa aggcgatctg
    29941 aaaggctttg agttcggcgt atttgactgg cacgccaacg gcacggcgac agatgcgaag
    30001 taatcattaa tcggcaactt tgggttgccg ccaaattgct aatatcgagt acgttgcttc
    30061 atgccggatg cggcgtaaac gccttatccg gcctataaga tccaaagaaa tcagtaaatt
    30121 gcaacacaca ttgtaggcct gataagcgta gcgcatcagg caatttctcg ttggtcatca
    30181 cttttgaaat caacttgacg attaacgctt ctcccagccg ccctgttgcg ccgtaaatcc
    30241 cagtgcctgc ataaacgctg tcatcacacc gcgatcttcc acgcctgcat ccgccatcca
    30301 ccagcatgaa acgccaggat tgttacgcaa aacctcttcc agcagatatt gccccacacc
    30361 gcgacgtcgg gtgacttccc gcacacgcag ggaatccagt gctccctcgg tgccgcttaa
    30421 ggttacccgt acggcagcga gcaggcgctc gttaaaacgc gcggcgtaga tacggtggtt
    30481 atcgtcaacc tgtaaagagg aaggggaata ctccggccag atcttttgca ggtcaatccg
    30541 gtcctggtcg ctaaagtttt ctaatcgaat gatggtcagc ttcatgggta acccgtgtaa
    30601 atcacaaaag tggaaccagt gtagcgaaat aatttaatcg gaggctttct cttttttatt
    30661 tcttttggca ggtgattaat tttttaacag caataattac aaagttaaaa cattagagaa
    30721 tgaaaaatac ccagcataat cccctgaatg gtagtgaatt attccgccct ttgtgccgtt
    30781 attttatgct gacaaaggca cttttttctg tttatctatc aataaattca gaatattatc
    30841 tgttcttaat cgactgaaaa atagggattt taatcactgt tatcacaaaa tactgcgcta
    30901 aacccttaat cagacaggca aaaacagtgc agtataaaaa aagaacagtc tgatttgtta
    30961 acacataaaa acaaagcaac acaacatcac gaatggggat ttttgactat gaaacggaat
    31021 gcgaaaacta tcatcgcagg gatgattgca ctggcaattt cacacaccgc tatggctgac
    31081 gatattaaag tcgccgttgt cggcgcgatg tccggcccga ttgcccagtg gggcgatatg
    31141 gaatttaacg gcgcgcgtca ggcgattaaa gacattaatg ccaaaggggg aattaagggc
    31201 gacaagctgg ttggcgtgga atatgacgac gcctgcgacc cgaaacaagc cgttgcggtc
    31261 gccaacaaaa tcgttaatga cggcattaaa tacgttattg gtcatctgtg ttcttcttct
    31321 acccaacctg catcagatat ctacgaagac gaaggtattc tgatgatctc gccgggagcg
    31381 accaacccgg agctgaccca acgcggttat caacacatta tgcgtactgc cgggctggac
    31441 tcttcccagg ggccaacggc ggcaaaatac attcttgaga cggtgaagcc ccagcgcatc
    31501 gccatcattc acgacaaaca acagtatggc gaagggctgg cgcgttcggt gcaggacggg
    31561 ctgaaagcgg ctaacgccaa cgtcgtcttc ttcgacggta ttaccgccgg ggagaaagat
    31621 ttctccgcgc tgatcgcccg cctgaaaaaa gaaaacatcg acttcgttta ctacggcggt
    31681 tactacccgg aaatggggca gatgctgcgc caggcccgtt ccgttggcct gaaaactcag
    31741 tttatggggc cggaaggtgt gggtaacgca tcattgtcga atattgctgg cgatgctgcc
    31801 gaaggcatgt tggtcactat gccaaaacgc tatgaccagg atccggcaaa ccagggcatc
    31861 gttgatgcgc tgaaagcaga caagaaagat ccgtccgggc cttatgtctg gatcacctac
    31921 gcggcggtgc aatctctggc gactgccctt gagcgtactg gcagcgatga gccgctggcg
    31981 ctggtgaaag atttaaaagc taacggtgca aacaccgtga ttgggccgct gaactgggat
    32041 gaaaaaggcg atcttaaggg atttgatttt ggtgtcttcc agtggcacgc cgatggttca
    32101 tccacggcag ccaagtgatc atcccaccgc ccgtgaaatg cgggcgggtt tagaaaggtt
    32161 accttatgtc tgagcagttt ttatatttct tgcagcagat gtttaacggc gtcacgctgg
    32221 gcagtaccta cgcgctgata gccatcggct acaccatggt ttacggcatt atcggcatga
    32281 tcaacttcgc ccacggcgag gtttacatga ttggtagcta tgtctccttt atgatcatcg
    32341 ccgcactgat gatgatgggc attgataccg gctggctgct ggtggcggcg ggattcgtcg
    32401 gcgcaatcgt cattgccagc gcctacggct ggagtatcga acgggtagcc tatcgcccgg
    32461 tgcgtaactc taagcgcctg attgcgctca tctcggcaat cggcatgtcc atcttcctgc
    32521 aaaactacgt tagcctgacc gaaggttcgc gtgacgtggc gctgcccagt ctgtttaacg
    32581 gtcagtgggt ggtggggcat agcgaaaact tctctgcctc tattaccacc atgcaggcag
    32641 tgatctggat tgttactttc ctcgccatgc tggcgctgac gattttcatt cgttattccc
    32701 gtatggggcg cgcctgccgt gcctgcgcgg aagatctgaa aatggcgagc ctgcttggca
    32761 tcaacaccga ccgggtgatt gctttgacct ttgttattgg cgcggcgatg gcggcagtgg
    32821 ctggcgtact gctcggtcag ttctacggcg tcattaaccc ctatatcggc tttatggccg
    32881 ggatgaaagc ctttaccgct gcggtgctcg gtgggattgg cagcattcca ggggcgatga
    32941 ttggcgggct gatcctgggg attgcggagg cgctctcttc tgcctatctg agtacggaat
    33001 ataaagatgt ggtctcattc gccctgctga ttctggtgct gctggtgatg ccgaccggta
    33061 ttctgggtcg cccggaggta gagaaagtat gaaaccgatg catattgcaa tggcactgct
    33121 ctctgccgcg atgttctttg tcctcgctgg cgtctttatg ggcgtgcaac tggagctgga
    33181 tggcaccaaa ctggtggtcg acagcgcttc ggatgtccgt tggcagtggg tgtttatcgg
    33241 cacggcggtg gtctttttct tccagctttt gcgacctgct ttccagaaag ggctaaaaag
    33301 cgtttccggg ccgaagttta ttctgcccgc gattgatggc tccacggtga agcagaaact
    33361 gttcctcgtg gcgctgctgg tgcttgcggt gtcgtggccg tttatggtgt cacgtgggac
    33421 ggtggatatc gccactctga ccatgatcta cattatcctc ggtctcgggc tgaatgtggt
    33481 tgttggtctt tctggtctgc tggtgctggg gtacggcggt ttttacgcca tcggcgctta
    33541 cacttttgcg ctgctcaatc actattacgg tctggggttc tggacctgtt tgccgattgc
    33601 cggactgatg gcagcggcgg cgggcttcct gcttggtttt ccggtgttgc gtctgcgcgg
    33661 tgattatctg gcgatcgtta ccctcggttt cggcgaaatt gtgcgcatat tgctgctcaa
    33721 taacaccgaa attaccggcg gcccgaacgg tatcagtcag atcccgaaac cgactttctt
    33781 cggccttgag ttcagccgta ccgcccgtga aggcggttgg gacacgttca gtaatttctt
    33841 tggcctgaaa tacgatccct ccgatcgcgt catcttcctg tatctggtgg cgttgctgct
    33901 ggtggtgcta agcctgtttg tcattaaccg cctgctgcgg atgccgctgg ggcgtgcgtg
    33961 ggaagcgttg cgtgaagatg agatcgcctg ccgttcgctg ggcttaagcc cgcgtcgtat
    34021 caagctgact gcctttacca tcagtgccgc gtttgccggt tttgccggaa cgctgtttgc
    34081 tgcgcgccag ggctttgtca gcccggaatc cttcaccttt gctgaatcgg cgtttgtact
    34141 ggcgatagtg gtgctcggcg gtatgggatc gcagtttgcg gtgattctgg cggcaatttt
    34201 gctggtggta tcgcgcgaat tgatgcgtga tttcaacgaa tacagcatgt taatgctcgg
    34261 tggtttgatg gtgctgatga tgatctggcg tccgcagggc ttgctgccca tgacgcgccc
    34321 gcaactgaag ctgaaaaacg gcgcagcgaa aggagagcag gcatgagtca gccattatta
    34381 tctgtgaacg gcctgatgat gcgcttcggc ggcctgctgg cggtgaacaa cgtcaatctt
    34441 gaactgtacc cgcaggagat cgtctcgtta atcggcccta acggtgccgg aaaaaccacg
    34501 gtttttaact gtctgaccgg attctacaaa cccaccggcg gcaccatttt actgcgcaat
    34561 cagcacctgg aaggtttacc ggggcagcaa attgcccgca tgggcgtggt gcgcaccttc
    34621 cagcatgtgc gtctgttccg tgaaatgacg gtaattgaaa acctgctggt ggcgcagcat
    34681 caacaactga aaaccgggct gttctctggc ctgttgaaaa cgccttcttt ccgtcgcgcc
    34741 cagagcgaag cgctcgaccg cgccgccacc tggcttgagc gcattggttt gctggaacac
    34801 gccaaccgtc aggcgagtaa cctggcctat ggtgaccaac gccgtctgga gattgcccgc
    34861 tgcatggtga cgcagccgga gattttaatg ctcgacgaac cggcggcagg tcttaaccca
    34921 aaagaaacca aagagctgga tgagctgatt gccgaactgc gcaatcatca caacaccact
    34981 atcttgttga ttgaacacga tatgaagctg gtgatgggga tttcagaccg aatttatgtg
    35041 gtcaatcagg ggacgccact ggcaaacggt acgccggagc aaatccgtaa taacccggac
    35101 gtgatccgtg cctatttagg tgaggcataa gatggaaaaa gtcatgttgt cctttgacaa
    35161 agtcagcgcc cactacggca aaatccaggc gctgcatgag gtgagcctgc atatcaatca
    35221 gggcgagatt gtcaccctca ttggcgcgaa cggggcgggg aaaaccacct tgctcggcac
    35281 gttatgcggc gatccgcgcg ctaccagcgg gcgaattgtg tttgatgata aagacattac
    35341 cgactggcag acggcgaaaa tcatgcgcga agcggtagcg attgtcccgg aagggcgtcg
    35401 cgtcttctcg cggatgacgg tggaagagaa cctggcgatg ggcggctttt ttgccgaacg
    35461 cgaccagttc caggagcgca taaagtgggt gtatgaactg ttcccacgtc tgcatgagcg
    35521 ccgtgttcag cgggcaggca ccatgtccgg cggtgagcaa caaatgctgg cgattggtcg
    35581 cgcgctgatg agcaacccgc gtttgctgct acttgatgag ccatcgcttg gtcttgcgcc
    35641 gattatcatc cagcaaattt tcgacaccat cgagcagctg cgcgagcagg ggatgactat
    35701 cttcctcgtc gagcagaacg ccaaccaggc gctaaagctg gcggatcgcg gctacgtgct
    35761 ggaaaacggc catgtggtgc tctccgatac tggtgatgca ctactggcga atgaagcggt
    35821 gagaagtgcg tatttaggcg ggtaataaca cgttgattga taaggagtca aaagactcct
    35881 ttgagacagg tgacaaatgt aaaattgcct gatgcgctgc gcttatcagg cctactgggt
    35941 gagttgcaat atattgaatt tgcacgatct tgtaggccgg ataaggcgtt tacgccgcat
    36001 ccggcatgaa acgatgagta atctgtagag tttgattcag accttctatt tttccgctta
    36061 tccgtgcccc atctcccatt ttccctcacc cacgccgtca ccgccttgtc atctttctga
    36121 caccttactt gcaggtttat gaagttggca ccgccaccat gatggcatcg aaagccatta
    36181 aaccggtata tgacgtgttt aaagaggcag ggattcaatt cgatgagtcg cagtttgtgc
    36241 cgacggtttc cggttactac tccgacagca aaacgggcca cttactctcc cagccattca
    36301 acagctcgac tcccgttctc tattacaaca aagacgcctt caagaaagcg gggttagacc
    36361 cggaacagcc gccgaaaacc tggcaggatc tggcggactc ttccgcaaaa ctgaaagcct
    36421 ccggtatgaa gtgcggctac gccagcggct ggcagggctg gatccaactg gaaaacttta
    36481 gcgcctggaa cggtctgccg tttgccagca aaaacaacgg ctttgacggc acggacgcgg
    36541 tgctggagtt caacaagccg gagcaggtga aacacattgc catgctcgaa gagatgaaca
    36601 agaagggcga cttcagctac gtcggtcgta aggatgaatc caccgagaag ttctataacg
    36661 gtgattgcgc gatgaccacc gcctcttccg gttctctcgc taatattcgt gagtacgcca
    36721 aatttaacta tggcgtaggc atgatgcctt acgacgccga tgcgaaagat gcaccgcaaa
    36781 acgccattat cggcggggcc agcctgtggg tgatgcaggg caaaaataaa gaaacgtata
    36841 ccggcgtggc gaagttcctc gacttcctcg cgaagccaga aaacgctgcc gagtggcatc
    36901 agaaaaccgg ttatctgcca atcaccaaag cagcgtatga cctgacccgt gagcagggct
    36961 tttatgagaa aaacccaggg gcggataccg cgacgcgtca gatgctgaat aagccgccgt
    37021 tgccgttcac caaagggctg cgtctgggca acatgccgca gatccgcgtg attgtggatg
    37081 aagagctgga gagcgtgtgg accggtaaga agacaccaca gcaggcactg gataccgccg
    37141 ttgagcgtgg aaatcagttg ctgcgccgct ttgagaaatc gacgaagtct taatcagtgt
    37201 aatgtcggat gcgtttcgct tatctgacct ggcatcgcgt gtaggccgga taagcgaagc
    37261 gcatccggca cagttcagga attaaccgta atgtcatcat cccgtccggt gttccgctcg
    37321 cgctggctgc cttatctgct ggtcgcgccg cagctcatca tcaccgttat cttttttatc
    37381 tggcctgcgg gcgaagcgtt gtggtactcg cggcaaagcg tcgatccgtt tggtttctcc
    37441 agccagtttg tcggtctgga taacttcgtc acgctgttcc atgacagcta ctatctagac
    37501 tccttctgga cgacgataaa attcagcacc tttgtcaccg tcagcggttt gctggtatcg
    37561 ctgttctttg cggcgctggt ggagtacatc gtgcgcggca gccgtttcta tcaaaccttg
    37621 atgctgctgc cgtatgccgt ggctcccgcc gttgccgccg tattgtggat cttcctgttt
    37681 aaccccggtc gcgggctgat cacccatttt ctcgctgagt tcggctacga ctggaaccac
    37741 gcgcaaaaca gcggtcaggc aatgtttctg gtggtgtttg cctcggtgtg gaagcaaatc
    37801 agctacaact ttctgttctt ctatgccgcg ctgcactcca ttccccgttc gttgattgaa
    37861 gccgcagcta ttgacggcgc agggccgatt cgccgcttct ttaagattgc tctgccgctt
    37921 atcgctccgg tgagtttctt cctgctggtg gtgaacctgg tgtacgcctt cttcgacacc
    37981 ttcccggtga tcgacgccgc cacgtccggc gggccggttc aggccaccac tacgctgatt
    38041 tataagatct accgcgaagg ctttaccgga ctggatctgg cttcgtctgc cgcgcagtcg
    38101 gtggtgttga tgttcctcgt catcgtgctg acggtggtgc agttccgcta tgttgaaagc
    38161 aaggtgcgtt accaatgatt gagaaccgtc cgtggctgac gatattcagc cataccatgc
    38221 tgatcctcgg gatcgcggtg atcctcttcc cgctgtacgt ggcgtttgtc gcggcgacgc
    38281 tggataaaca ggccgtctat gccgcgccga tgacgctcat ccccggcaca catctgctgg
    38341 aaaatatcca caacatctgg gttaacgggg taggcacgaa tagcgcgccg ttctggcgga
    38401 tgttgcttaa cagctttgtg atggcgttca gcattacgct cggcaaaatt accgtctcga
    38461 tgctctcggc atttgccatt gtctggtttc gttttccgct acgtaacctc ttcttctgga
    38521 tgatttttat caccctgatg ctgccggttg aagtacgtat cttcccgacg gtggaagtca
    38581 tcgccaacct gaaaatgctc gacagctacg ccggtttaac gctgccgctg atggcctcgg
    38641 cgaccgccac gtttctgttc cgccagttct ttatgaccct accggatgaa ttggtggaag
    38701 ccgcgcggat cgacggcgcg tcaccaatgc gtttcttttg cgacatcgtt tttccgttgt
    38761 ccaaaactaa tctggcggcg ctgtttgtga tcacctttat ctacggctgg aatcagtatt
    38821 tgtggccgtt gttgattatt accgacgttg atctcggcac caccgtggca gggatcaaag
    38881 ggatgatcgc tacaggcgaa ggcaccacgg aatggaactc ggtaatggcg gcgatgttgt
    38941 taacgcttat ccctccggtg gtgattgttt tagtgatgca gcgtgccttc gtgcgcggcc
    39001 tggtcgatag tgagaaataa gatggcagga ctgaaattac aggcagtaac caaaagctgg
    39061 gatggcaaaa cccaggtcat taaaccgctg acccttgatg tggcggatgg cgaatttatc
    39121 gtgatggtcg ggccgtctgg ctgcgggaaa tcgacgctgc tgcgcatggt tgccgggctg
    39181 gagcgggtga cagaaggcga tatctggatc aacgaccagc gcgtgaccga aatggagccg
    39241 aaagatcgcg ggattgcgat ggtgttccag aattacgcgc tttatccgca tatgagcgta
    39301 gaggagaaca tggcgtgggg gctgaaaatt cgcggcatgg gcaagcagca aattgccgag
    39361 cgcgttaaag aagcggcgcg cattctggaa ctggacggtc tgcttaagcg tcgtccgcgt
    39421 gagctatccg gcggtcagcg ccagcgtgtg gcgatgggcc gcgcgattgt gcgcgatccg
    39481 gcggtgttct tgtttgatga gccgctctct aacctcgatg ccaagctgcg cgtgcagatg
    39541 cgtcttgaac tgcaacagtt gcaccgtcgc ctgaaaacga cttcactcta cgttactcac
    39601 gatcaggttg aagcgatgac gctcgcccag cgagtaatgg tgatgaacgg tggcgttgcc
    39661 gaacagattg gcacaccagt tgaagtctac gaaaagcccg ccagcctgtt tgtggcgagt
    39721 tttatcggca gcccggcgat gaacctgctg acaggccgcg tgaataatga aggcacgcat
    39781 ttcgaactgg acggcggtat cgtgctaccg ctaaacggtg gctaccgtca gtatgccggg
    39841 cgtaaaatga ctctcggcat tcgcccggaa catatagcgc taagctcgca ggcagaaggt
    39901 ggtgtgccgc tggtgatgga cacgctggag atcctcggcg cagataacct ggcgcacgga
    39961 cgctggggcg agcagaagct ggtggtgcga ctggcgcatc aggagcgccc gacggcaggc
    40021 agcacgctgt ggctgcattt ggcggaaaat cagctgcatc tttttgatgg tgaaacagga
    40081 caacgagtat gagtaactgg ccttatcccc gcatcgtcgc tcatcgtggc ggcggtaagc
    40141 tggccccgga aaacaccctg gcggcaatcg acgtcggggc aaaatacggt cataagatga
    40201 tcgaatttga cgcgaagtta tcgaaagatg gcgagatctt cctgctccat gacgacaatc
    40261 tcgaacgcac cagcaacggc tggggcgtcg cgggtgaact gaactggcag gatttactgc
    40321 gcgtggatgc gggcagttgg tacagcaaag cgtttaaagg tgagccgctg ccgttgcttt
    40381 cgcaggtggc ggaacgctgt cgcgaacacg ggatgatggc gaatatcgaa atcaaaccca
    40441 ccactggcac tggaccatta acgggcaaaa tggtggcgct ggcggcacgc caactgtggg
    40501 ccggtatgac gccgccgctg ctgtcatcgt ttgagattga tgctttagaa gctgcacaac
    40561 aggcggcacc ggaactgccg cgtggtttat tgcttgatga gtggcgcgac gactggcgcg
    40621 aactgaccgc gcggctgggc tgcgtctcta ttcatctcaa tcataagtta ctcgataaag
    40681 cgcgagtgat gcagttgaaa gacgccggat tacggattct ggtttatacc gtcaataaac
    40741 cccagcgcgc ggcagagttg ctgcgctggg gcgtggattg catctgtacc gatgcgattg
    40801 acgtgattgg tccgaacttt acggcccaat agttttcaac ggaatgtcag gctgcggcgt
    40861 gctgggctga ttcagcatgt cgccgtttct cctttccggc agcatatgct gctgattcaa
    40921 cgaactatcc ggattacggt tgctgtttaa catcccgccg ttggtgttgg gcagcatttg
    40981 ctgccgggcg ggatttcgct cccccggctg tgactgcaac acccgctgag aattattgtt
    41041 tatctggttt tctaaatgct gctgttgcaa ctgagtttgc gttttcagtt gctgattcag
    41101 catccctttt tgctggattt gctgagtctg catctgggtt tgcatccgct gctggctggg
    41161 tatctgatac cccggctggt tagggttgtt cagagtatta atgggctgtg caaagccgac
    41221 aaacggcagg agtgccgtaa gaatcagaag tcgtttcatc gcgtatcctc ctctgaagat
    41281 atcctttaag tttactcgct tcccgacaaa acgatgatta attcagagtt atataccagg
    41341 cttagctggg gttgcccctt aatctctgga gaataacgat gataaaaccg acgtttttac
    41401 gccgggtggc cattgctgct ctgctctcag gaagttgttt tagcgccgcc gccgcgcctc
    41461 ctgcgccgcc cgtctcgtat ggtgtggagg aagatgtctt ccacccggta cgcgcgaaac
    41521 agggaatggt agcgtctgtg gacgccactg ccactcaggt gggggtggat atcctcaagg
    41581 agggcgggaa tgccgttgat gccgccgtgg cggtgggcta cgcgctggcg gtaacgcatc
    41641 cgcaggcagg gaatctgggc ggtggtggtt ttatgttaat ccgctcgaaa aatggcaata
    41701 ccacggctat cgatttccgc gaaatggcac ccgccaaagc gacccgcgat atgttcctcg
    41761 atgatcaggg caacccggac agcaaaaaat cactcacttc gcatctggct tccggcacac
    41821 cgggtacggt agcaggtttc tcgctggcgc tggataaata cggcaccatg ccgctgaaca
    41881 aagtcgtgca gcccgcgttt aaactggcac gcgatggttt tatcgttaac gacgcgctgg
    41941 ctgacgatct caaaacctac ggtagcgaag tgttgccgaa tcacgaaaac agtaaagcta
    42001 tcttctggaa agagggcgag ccgctgaaaa agggcgacac gctggtgcag gcgaacctgg
    42061 caaagagcct ggagatgatt gctgaaaacg gcccggacga attctataaa ggcacgattg
    42121 cggaacagat cgcccaggag atgcagaaaa acggtggctt gatcactaaa gaagatttag
    42181 cggcctataa agcggtcgaa cgcactccga taagcggcga ttatcgcggg tatcaggttt
    42241 actccatgcc accgccatcc tccggcggga tccatatcgt acaaatcctc aatattctgg
    42301 aaaacttcga tatgaagaaa tacggctttg gcagcgccga tgcgatgcaa atcatggcag
    42361 aagcggagaa atacgcctac gccgaccgct cggaatatct tggcgacccg gattttgtca
    42421 aagtcccgtg gcaggcactg accaataaag cctatgccaa atccattgcc gatcaaattg
    42481 atatcaacaa agcgaagccg tccagtgaga ttcgccctgg caagcttgcg ccttatgaga
    42541 gtaatcaaac tacccattac tctgtagtgg ataaagacgg taacgcggtg gcggtgacct
    42601 atacgctgaa caccaccttc ggtacgggca ttgtcgcggg cgagagcggt attctgctta
    42661 ataatcagat ggatgatttc tccgccaaac cgggcgtacc gaacatttac gggctggtgg
    42721 gcggtgatgc caacgccgtc gggccgaata aacgtccgct gtcatcgatg tcaccaacca
    42781 ttgtggtgaa agacggtaaa acttggctgg ttaccggtag tcctggcggg agtcggatta
    42841 ttaccaccgt gc
//
LOCUS       DADMKF010000016        42611 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.016, whole genome shotgun sequence.
ACCESSION   DADMKF010000016 DADMKF010000000
VERSION     DADMKF010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 42611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42611
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.016"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            54..1274
                     /locus_tag="JNC24_000807"
                     /pseudo
     CDS             54..1274
                     /locus_tag="JNC24_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
     gene            1247..1876
                     /locus_tag="JNC24_000808"
     CDS             1247..1876
                     /locus_tag="JNC24_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
                     /protein_id="HAY9157633.1"
                     /translation="MGDTMQQRLTQDLTQFLASLPEDDRIKAINEIRMAIHQVSPFRE
                     EPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLQKSIEIDGFTQPIVVTHTDKNAMEIV
                     DGFHRHEIGKGSSSLKLRLKGYLPVTCLEGTRNQRIAATIRHNRARGRHQITAMSEIV
                     RELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADRQYSRAWTVK"
     gene            complement(1877..3037)
                     /gene="ybdL"
                     /locus_tag="JNC24_000809"
     CDS             complement(1877..3037)
                     /gene="ybdL"
                     /locus_tag="JNC24_000809"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HAY9157634.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTQMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYAPAIALSGGIVKRMALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNSSATVWQQADFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHSQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     LADMLRAEPEHYLALPDFYRQKRDILVNALNESRLEILPCEGTYFLLVDYSAVSTLDD
                     VEFCQWLTQEHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            3146..4234
                     /gene="hcxA"
                     /locus_tag="JNC24_000810"
     CDS             3146..4234
                     /gene="hcxA"
                     /locus_tag="JNC24_000810"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HAY9157635.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLNDFFTDEQLSRAVWIYGKR
                     AIAAAQTKLPPAFGLPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAVPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALS
                     DQQNQQLTQSFCDVVDAIIAGGGMVGDLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEMDINNHAEIDKVITHTLRP
                     VEYIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(4244..4441)
                     /locus_tag="JNC24_000811"
     CDS             complement(4244..4441)
                     /locus_tag="JNC24_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HAY9157636.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(4623..6728)
                     /gene="cstA"
                     /locus_tag="JNC24_000812"
     CDS             complement(4623..6728)
                     /gene="cstA"
                     /locus_tag="JNC24_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein CstA"
                     /protein_id="HAY9157637.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLAIFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFAITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQGVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTAGWQKAFSPDAKVGFLAIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKSALAALKDPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            complement(6909..7322)
                     /gene="entH"
                     /locus_tag="JNC24_000813"
     CDS             complement(6909..7322)
                     /gene="entH"
                     /locus_tag="JNC24_000813"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HAY9157638.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTRLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPMSEGKVRGVC
                     QPLHLGRQNQSWEIIVFDEQGRRCCTCRLGTAVLG"
     gene            complement(7325..8071)
                     /gene="dhbA"
                     /locus_tag="JNC24_000814"
     CDS             complement(7325..8071)
                     /gene="dhbA"
                     /locus_tag="JNC24_000814"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase"
                     /protein_id="HAY9157639.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALAFVEAGAKVTGFDQAFAQEQY
                     PFATEVMDVADAAQVAQVCQRLLAETERLDVLVNAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTPRIGMSAYGASKAALKSLALSVGL
                     ELAVSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(8071..8928)
                     /gene="entB"
                     /locus_tag="JNC24_000815"
     CDS             complement(8071..8928)
                     /gene="entB"
                     /locus_tag="JNC24_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HAY9157640.1"
                     /translation="MAIPKLQAYALPESHDIPQNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEGLLPAPVPARKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(8942..10552)
                     /gene="entE"
                     /locus_tag="JNC24_000816"
     CDS             complement(8942..10552)
                     /gene="entE"
                     /locus_tag="JNC24_000816"
                     /EC_number="2.7.7.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase"
                     /protein_id="HAY9157641.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVTEHSSIRVVQLLNDSGE
                     HNLQDAINHPADGFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEIC
                     QFTQQTRYLCAIPAAHNYAMSSPGSLGVFLAGGTVVLAADPSATLCFPLIAKHQVNVT
                     ALVPPAVSLWLQALAEGESRAQLASLKLLQVGGARLSATLAARISAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEEIENL
                     LLRHPAVIYAALVSMEDELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            complement(10562..11737)
                     /gene="entC"
                     /locus_tag="JNC24_000817"
     CDS             complement(10562..11737)
                     /gene="entC"
                     /locus_tag="JNC24_000817"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
                     /protein_id="HAY9157642.1"
                     /translation="MDTSLAEEVQQTMATLAPNRFFFMSPYRSFTTSGCFARFDEPAV
                     NGDSPDSPFQQKLAALFADAKAQGIKNPVMVGAIPFDPRQPSSLYIPESWQSFSRQEK
                     QTSAHCFTRSQSLNVVERQAIPEQTTFEQMVARAAALTATPQVDKVVLSRLIDITTDA
                     AIDSGVLLERLIAQNPVSYNFHVPLADGGVLLGASPELLLRKDGERFSSIPLAGSARR
                     QPDEVLDREAGNRLLASEKDRHEHELVTQAMKEVLRERSSELHVPSSPQLITTPTLWH
                     LATPFEGKANSQENALTLACLLHPTPALSGFPHQAATQVIAELEPFDRELFGGIVGWC
                     DSEGNGEWVVTIRCAKLRENQVRLFAGAGIVPASSPLGEWRETGVKLSTMLNVFGLH"
     gene            12112..13077
                     /gene="fepB"
                     /locus_tag="JNC24_000818"
     CDS             12112..13077
                     /gene="fepB"
                     /locus_tag="JNC24_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HAY9157643.1"
                     /translation="MRLAPLYRNALLLTGLLLSGIAVVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSAEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYTLGTETFRLDYYSAMQVLDRLNSLLKKD"
     gene            complement(13158..14408)
                     /gene="entS"
                     /locus_tag="JNC24_000819"
     CDS             complement(13158..14408)
                     /gene="entS"
                     /locus_tag="JNC24_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HAY9157644.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATPALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPALPPPPQPREHPLKSLLAGFR
                     FLLASPLVGGIALLGGLLTMASAVRVLYPALADNWQMSAAQIGFLYAAIPLGAAIGAL
                     TSGKLAHSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYT
                     MLQTQTPEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGV
                     LLLLVLVELRRFRQTPPQVTASDS"
     gene            14519..15523
                     /gene="fepD"
                     /locus_tag="JNC24_000820"
     CDS             14519..15523
                     /gene="fepD"
                     /locus_tag="JNC24_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HAY9157645.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAF
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFIGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            15520..16512
                     /gene="fepG"
                     /locus_tag="JNC24_000821"
     CDS             15520..16512
                     /gene="fepG"
                     /locus_tag="JNC24_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HAY9157646.1"
                     /translation="MIYVSRRLLITCLLLISACVVAGIWGLRSGAVTLEISQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMVGGIVTSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAAALLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAADLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            16509..17324
                     /gene="fepC"
                     /locus_tag="JNC24_000822"
     CDS             16509..17324
                     /gene="fepC"
                     /locus_tag="JNC24_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HAY9157647.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEEAVTKAMQATRITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGTPLVVPLGRTAPSTANS"
     gene            complement(17321..18454)
                     /gene="fepE"
                     /locus_tag="JNC24_000823"
     CDS             complement(17321..18454)
                     /gene="fepE"
                     /locus_tag="JNC24_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
                     /protein_id="HAY9157648.1"
                     /translation="MSSLNIKQGSDAHFSEYPLASPSNNEIDLLNLIEVLWRAKKTVM
                     AVVFAFACAGLLISFILPQKWTSSAVITPAEAIQWQDLEKTFTKLRVLDLDVNIDRGG
                     AFNLFIKKFQSVSLLEEYLRSSPYVMDQLKEAKIDELDLHRAIVALSEKMKAVDDNAS
                     KKKDEPSLYTSWTLSFTAPTSKEAQTVLSGYIDYISALVVKESIENVRNKLEIKTQFE
                     KEKLAQDRIKTKNQLDANIQRLNYSLDIANAAGIKKPVYSNGQAVKDDPDFSISLGAD
                     GIERKLEIEKAVTDVAELNGELRNRQYLVEQLTKANVNDVNFTPFKYQLSPSLPVKKD
                     GPGKAIIVILSALIGGMVACGSVLLRYAMASRKQDAMMADHLV"
     gene            complement(18670..22551)
                     /gene="entF"
                     /locus_tag="JNC24_000824"
     CDS             complement(18670..22551)
                     /gene="entF"
                     /locus_tag="JNC24_000824"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HAY9157649.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGDVDAPLL
                     ARAVVAGLAQADTLRMRFTEDNGEVWQWVDDALIFEQPEIIDLRTNIDPHGTAQALML
                     ADLQQDLRVDSGKPLVFHQLIHVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCTW
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWAEQRRQLPPPASLSPAPLPGR
                     SASADILRLKLEFTDGEFRQLATQLSGVQRTDLALALAALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTASGPVLNVLPLGIHIAAQETLPELATRLAAQLKKMRRHQRYDAEQIVRD
                     SGRAAGDEPLFGPVLNIKVFDYQLDIPGVQAQTHTLATGPVNDLELALFPDEHGDLSI
                     EILANKQRYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQINATQV
                     EIPETTLSALVAEQAAKTPDAPALADARYLFSYREMREQVVALANLLRERGVKPGDSV
                     AVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQLPRF
                     SDVPNLTSLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAIVNRL
                     LWMQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAMQQFF
                     AEYGVTTTHFVPSMLAAFVASLTPQTARQSCATLKQVFCSGEALPADLCREWQQLTGA
                     PLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNMGLRILDAMMHPVPPGV
                     AGDLYLTGIQLAQGYLGRPDLTASRFIADPFAPGERMYRTGDVARWLDNGAVEYLGRS
                     DDQLKIRGQRIELGEIDRVMQALPDVEQAVTHACVINQAAATGGDARQLVGYLVSQSG
                     LPLDTSALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKTQASGRAP
                     KAGSETIIAAAFASLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQFARQVTPGQVM
                     VASTVAKLATIIDGEEDSSRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVLSRYL
                     DPQWSIIGIQSPRPHGPMQTATNLDEVCEAHLATLLEQQPHGPYYLLGYSLGGTLAQG
                     IAARLRARGEQVAFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGS
                     TSTELFTTIEGNYADAVRLLTTAHSVPFDGKATLFVAERTLQEGMSPERAWSPWIAEL
                     DIYRQDCAHVDIISPGAFVKIGPIIRATLNR"
     gene            complement(22548..22766)
                     /locus_tag="JNC24_000825"
     CDS             complement(22548..22766)
                     /locus_tag="JNC24_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbtH family protein"
                     /protein_id="HAY9157650.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCLLSAGWDIVCQPQ
                     SQASCQQWLEVHWRTLTPANFTQLQEAQ"
     gene            complement(22769..23971)
                     /gene="fes"
                     /locus_tag="JNC24_000826"
     CDS             complement(22769..23971)
                     /gene="fes"
                     /locus_tag="JNC24_000826"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HAY9157651.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEEYS
                     TIKRVWIYITGVTDHHQNSQPQSMQRIAGTDVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGRGHAVSALEMPQAPLQPGWDCPQV
                     PEIAAKEIIWKSERLKNSRRVWIFTTGDVTAEERPLAVLLDGEFWAQSMPVWPVLTSL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKAIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRDGQQEGVLLEKLKAGEVSAEG
                     LRIVLEAGIREPMIMRANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            24214..26454
                     /gene="fepA"
                     /locus_tag="JNC24_000827"
     CDS             24214..26454
                     /gene="fepA"
                     /locus_tag="JNC24_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HAY9157652.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDSYTRSKYGDETNRLYRQNYSLTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDIDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGANTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKMGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYQQGNDDLKAETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSEMVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SVNTHF"
     gene            26629..27249
                     /gene="entD"
                     /locus_tag="JNC24_000828"
     CDS             26629..27249
                     /gene="entD"
                     /locus_tag="JNC24_000828"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HAY9157653.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGR
                     KRKTEHLAGRIAAVYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGTTALAVVSRQ
                     PIGIDIEEIFSVQTARELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASER
                     QTEAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
     gene            complement(27371..27523)
                     /locus_tag="JNC24_000829"
     CDS             complement(27371..27523)
                     /locus_tag="JNC24_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HAY9157654.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            complement(27874..28026)
                     /locus_tag="JNC24_000830"
     CDS             complement(27874..28026)
                     /locus_tag="JNC24_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HAY9157655.1"
                     /translation="MLTKYALAAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            28466..29584
                     /locus_tag="JNC24_000831"
     CDS             28466..29584
                     /locus_tag="JNC24_000831"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HAY9157656.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNK
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTHPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFASSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRPLTEDTLRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWADD"
     gene            29650..29898
                     /locus_tag="JNC24_000832"
                     /pseudo
     CDS             29650..29898
                     /locus_tag="JNC24_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
     gene            29963..30331
                     /locus_tag="JNC24_000833"
     CDS             29963..30331
                     /locus_tag="JNC24_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HAY9157657.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYPGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            30425..31078
                     /gene="nfsB"
                     /locus_tag="JNC24_000834"
     CDS             30425..31078
                     /gene="nfsB"
                     /locus_tag="JNC24_000834"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HAY9157658.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNERKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNITLTEV"
     gene            31186..32433
                     /locus_tag="JNC24_000835"
     CDS             31186..32433
                     /locus_tag="JNC24_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HAY9157659.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(32501..33877)
                     /gene="pheP"
                     /locus_tag="JNC24_000836"
     CDS             complement(32501..33877)
                     /gene="pheP"
                     /locus_tag="JNC24_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HAY9157660.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPDKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(33979..37122)
                     /gene="cusA"
                     /locus_tag="JNC24_000837"
     CDS             complement(33979..37122)
                     /gene="cusA"
                     /locus_tag="JNC24_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HAY9157661.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKARENGVPVYLRDVAKVQIGPEMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIASVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCALFLWHVRSALVAIISLPLGLCIAFIVMHFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQDQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDAMAEQIEEVARTVPSVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADVKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRK"
     gene            complement(37134..38357)
                     /gene="cusB"
                     /locus_tag="JNC24_000838"
     CDS             complement(37134..38357)
                     /gene="cusB"
                     /locus_tag="JNC24_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
                     /protein_id="HAY9157662.1"
                     /translation="MKKIALIIGSMIAGGIISAAGFTWFAKAEPPAEKTSTAERKILF
                     WYDPMYPNTRFDKPGKSPFMDMDLVPKYADEESSASGVRIDPTQTQNLGVKTATVTRG
                     PLTFAQSFPANVSYNEYQYAIVQARAAGFIDKVYPLTVGDKVQKGTPLLDLTIPDWVE
                     AQSEYLLLRETGGTATQTEGILERLRLAGMPEADIRRLIATQKIQTRFTLKAPIDGVI
                     TAFDLRAGMNIAKDNVVAKIQGMDPVWVTAAIPESIAWLVKDASQFTLTVPARPDKTL
                     TIRKWTLLPGVDAATRTLQLRLEVDNADEALKPGMNAWLQLNTASEPMLLIPSQALID
                     TGNEQRVITVDADGRFVPKRVAVFQASQGVTALHSGLAEGEKVVSSGLFLIDSEANIS
                     GALERMRSESATNAH"
     gene            complement(38373..38705)
                     /gene="cusF"
                     /locus_tag="JNC24_000839"
     CDS             complement(38373..38705)
                     /gene="cusF"
                     /locus_tag="JNC24_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HAY9157663.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEVQPQVISATGVV
                     KGIDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(38729..40111)
                     /gene="cusC"
                     /locus_tag="JNC24_000840"
     CDS             complement(38729..40111)
                     /gene="cusC"
                     /locus_tag="JNC24_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HAY9157664.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRTAMPVPQQFSLSQNGLVN
                     AADNYQNVGWRTFFVDNQVKTLISEALVNNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGNTATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSGSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLNDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEISL
                     YTALGGGWQQ"
     gene            40268..40951
                     /gene="cusR"
                     /locus_tag="JNC24_000841"
     CDS             40268..40951
                     /gene="cusR"
                     /locus_tag="JNC24_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HAY9157665.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            40941..42389
                     /gene="cusS"
                     /locus_tag="JNC24_000842"
     CDS             40941..42389
                     /gene="cusS"
                     /locus_tag="JNC24_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HAY9157666.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFTRDAIPDKDAQGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTEPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDKCQVAGDPLMLRRALSNLLSNALRYTP
                     TRETIVVRCQTVDHQVQVTVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVIMLPERE"
BASE COUNT        10229 a        11777 c        10856 g         9749 t
ORIGIN      
        1 catcaccgtc ttatctcact gttcaccctg taacgttcag agagcgtctt cagatgtcta
       61 tttataaaat tcctcttccg ctcaatattc tggaagctgc acgagagcgt attacctgga
      121 cactcaacac cctaccccgc gtttgtgtct cattttctgg cggcaaaaac tctggtttaa
      181 tgctgcatct gacagccgaa cttgcccgac aaatgggcaa aaaaatctgc gttttgttta
      241 tcgactggga ggcgcaattc tcttgcacca ttaactatgt tcagtccctg cgcgagttgt
      301 acaccgatgt catcgaagag ttttactggg ttgcgctccc gcttacgacg caaaattccc
      361 tttcacaata ccaacccgaa tggcagtgct gggaacctga tgtcgaatgg gtgcgtcagc
      421 ccccgcaaga tgcgataacc gatcctgact ttttctgctt ttaccagcca ggcatgacct
      481 tcgaacaatt tgtacgtgag tttgccgaat ggttttcaca aaaacgtccg gtggcgatga
      541 tgatcggcat ccgtgcggat gagtcctaca accgttttgt cgccatcgcc agtttaaata
      601 aacaacgttt tgccgacgat aaaccatgga ctacggccgc accaggcggt catagctggt
      661 acatttaccc catttacgac tggaaagtgg ctgatatctg gacctggtat gcaaatcatc
      721 aaaatctctg caacccactg tataacctaa tgtatcaggc aggcgttcct ctgcgtcata
      781 tgcgaatttg agaacctttt ggcccggagc aacgacaagg attatggctc tatcacgtta
      841 tcgaaccgga tcgctgggct gctatgtgcg cacgagtcag cggggtaaaa agtggcggca
      901 tttacgccgg acatgataat catttctatg ggcatcggaa aatcctcaag ccagaacatt
      961 tagactggca agaatatgca ttattgctgc tcaatagcat gccggaaaaa acagctgagc
     1021 attaccgcaa taaaatcgcc atttatttgc actggtatca gaaaaaaggc atcgaggttc
     1081 cacaaaccca gcaaggggac attggcgcga aagatatccc ctcctggcgg cggatatgca
     1141 aagttttact caataacgat tactggtgtc gggcattatc atttagtcct acgaaagcga
     1201 agaactatca gcgttataac gaacggataa aaggaaaacg tcaggaatgg gggatactat
     1261 gcaacaacga ttaacgcagg atttaacgca atttctcgcc agcttgccgg aagatgatcg
     1321 catcaaagcg attaatgaaa tccgcatggc gatccatcag gttagcccct ttcgtgaaga
     1381 gccagtggat tgcgttctgt gggttaaaaa cagccaactt atgcctaatg attacaaccc
     1441 aaacaacgtg gcaccacccg aaaaaaaact gctgcaaaaa tccattgaaa tagatggttt
     1501 tacccaaccc attgtggtca cacatacgga taaaaatgcc atggaaatcg tggatggttt
     1561 tcaccgccat gaaattggta aaggctcatc atcattaaaa ttacgcttaa aaggttattt
     1621 gccggtgacc tgtctggaag gtacgcgtaa tcaacgcatc gccgcgacta ttcgtcataa
     1681 tcgcgctcgt ggacgccatc aaatcaccgc catgtcggaa atcgtccgtg aacttagcca
     1741 gttgggatgg gacgataata aaattggcaa agagctggga atggacagcg acgaagtgct
     1801 gcgcctgaag caaattaacg gcctgcaaga gctgtttgcc gaccgtcaat attcccgcgc
     1861 ctggacggtt aaataactaa agctggcgca ggcgttcagc tgctgccagc aacgtcgatt
     1921 ccttcttggc aaaacagaga cgaatcagtt tatgtgggaa gggatcggcg caaaacaccg
     1981 acagcggaat cgccgctacg ccgtgctcct gcgtcagcca ctggcaaaac tcaacatcat
     2041 ccagggtaga aaccgcgctg taatccacca gcaaaaagta tgtaccttca cacggtaaaa
     2101 tctccagccg gctttcattt aaggcattta ccagaatatc gcgcttctgg cgataaaagt
     2161 ccggtaacgc aagataatgc tcaggttctg cacgtagcat atcagcaagc gccagctgtg
     2221 ccggggtatt caccgaaaag gtcagatact gatgtacctt gcgaatttcg gcgctgatgg
     2281 gcgctggcgc aacacaataa cccactttcc agccggtcat atgataggtc ttgccaaatg
     2341 aagaaaccgc cactgcccgc tcacgcagct gcgaatgcgc cagcacactg gcatggccct
     2401 gttgtgaaaa gttgatgtgc tcgtagactt catcgctaat gacaaaaatc tcgtgcccgg
     2461 cgatcgcctg ccacaaagcg gcgaaatcag cctgctgcca gacagttgca ctggagttat
     2521 gcggagtgtt gaggatcacc agtctggtgc gctcgcttaa taatgcggca aattcctgcc
     2581 agtcaacgcg aaaatgcggt ggttgcagtg ccatacgctt cactattccc ccagaaagcg
     2641 cgatggcggg ggcgtaactg tcatagctgg gatcaaaaca aatcacttca tcgccattgc
     2701 gcaccagtgc ggtaatcgcc gcgtataacg cttccgtcgc ccctgccgtt acggtgatat
     2761 cgctatcggc atctggttga tagccataca aacgttccgt tttctgagca atcgcctcgc
     2821 gcaaggcctg cacgccggtc atgggcgcgt attggtttgc cccctgggca acgtggtacg
     2881 ccagccgctc ctgtaaatag cgcggaccat caaaatcagg aaagccttgc gacaggttaa
     2941 tcgcctggtg ttgctgcgcc agcgcgctca tctgggtgaa aatagtggtg ccaagttgtg
     3001 gaagtttgct ttgtggaatc agagggttat ttgtcatttt tattgtacct gacgaagttg
     3061 cgtgttgaag gcactataac acgatgctag tatttggcaa tcaagacgtt tagatgtcta
     3121 aataaaacaa taaggacaac acaacatgcc tcacaatcct atccgcgtgg tcgtcggccc
     3181 ggctaactac ttttcacatc caggaagttt caatcacctg aacgattttt tcactgatga
     3241 acaactttct cgcgcggtgt ggatctacgg caaacgcgcc attgctgcgg cgcaaaccaa
     3301 acttccgcca gcgtttggac ttccaggggc aaagcatatt ttgtttcgtg gtcattgcag
     3361 cgaaagcgat gtacagcaac tggcggctga gtccggtgac gatcgcagcg tggtgattgg
     3421 cgtcggcggc ggcgcactgc tcgacaccgc gaaagccctc gcccgccgtc tcggtctgcc
     3481 atttgttgcc gttccgacga tcgccgctac ctgcgccgcc tggacaccgc tctccgtctg
     3541 gtataacgat gccggacagg cgctgcatta tgagattttc gacgacgcca attttatggt
     3601 gctggtggaa ccagagatta tcctcaacgc gccgcaacaa tatctgctgg cggggatcgg
     3661 tgacacgctg gcgaaatggt atgaagcggt ggtgctggct ccgcaaccag aaacgttgcc
     3721 gctaaccgtg cgactgggga tcaataatgc gcaggccatt cgcgacgtat tgttaaacag
     3781 tagcgaacag gcgctgagcg atcagcaaaa tcaacagtta acgcaatcat tttgcgatgt
     3841 ggtggatgct attattgctg gtggtgggat ggtcggtgat ctgggcgatc gttttacgcg
     3901 tgtggcggcg gctcatgccg tgcataacgg tctgactgtc ctgccgcaaa ccgagaaatt
     3961 tctccacggc accaaagtcg cttatggcat tctggtacaa agcgccttgc taggtcagga
     4021 tgatgtgctg gcgcaattaa ccggggcgta tcagcgtttt catctgccga ccacgctggc
     4081 ggagctggaa atggatatca ataatcatgc ggagatcgac aaagtgatta cccacaccct
     4141 gcgtccggtg gaatacattc attacctgcc agtcacgctg acaccagata cgttgcgtgc
     4201 agcgttcgaa aaagtggaat cgtttaaagc ctgacgtgca aatttagcag cagcgcgcgc
     4261 cgccttttcc accgtagcgc gcgtcctgcc gctcacggaa aaactcttcg taggtcatcg
     4321 gcgtttgatc gggatggtta acccgcatat gttcgacata gttgtcgtaa tcaggcatac
     4381 caatcatcag cttcgccgcc tgacctaaat attttccggc ttttgccagt gaatcaaaca
     4441 tcttggcctc cgctaaccat gttgctgaaa gagtgccgga tgcggcgtga acgccttatc
     4501 cggcctacaa atgctatcaa cgcgctcggt cagtcccctc gccccatcgg ggagagggtt
     4561 agggtgagga ggaaccctcg gcactatgcc aacattcgcc aacatccccc ctcactctga
     4621 ctttagtgtg cgccttttgc ctgcgccacg atctcctcga cattttccgg cattggctca
     4681 tacggcgttt ctttcgccgt tggcttcgga tctttcaatg ccgccagtgc cgacttaatc
     4741 gagaacagcg ccagaaccac cacgaccacc ataaagaaga tggttaaccc ggcatccaga
     4801 cggttgttga acaccagttg cgccagctgt gactcagtat actgcgacgg aatattgccg
     4861 ctgtctatca ttgcctggaa cttattagca atggccagga agccgacttt cgcatccggg
     4921 ctaaacgctt tctgccagcc tgcggtcagg gtacaaatca gcagccaggc cgttggtacc
     4981 agcgccaccc aggcgtaacg ttgacgtttc atcttgaaca acaccacggc acagagcatc
     5041 agcgccatcc ctgccagcat ctggttggca ataccaaaca gcggccacag agtgttaatg
     5101 ccgcccagcg gatcgaccac gccctgatgg aggaagtagc cccacgccag tacgcacagc
     5161 gctgttgcca gcaggttagc aggcagtgaa tcggtccgct tcaggccagg agacaccacg
     5221 cccagcagat cctgcaacat aaagcgcgca gcacgcgtac ccgcatctac cgccgtcaga
     5281 ataaacagag cttcaaacag aatggcgaag tgataccaga acgccacatc catcatacca
     5341 cccagcgcac cgtgcaggat gtaagccatc cccaccgcca gcgtcggcgc accgcccgca
     5401 cgggaaatga tcgactgttc acccacttcg ctggcaatct ggttgagcgt atccggagta
     5461 atcgcaaagc cccagctact caccacctgc gcggcagaag cgaccacatc cgccgtccct
     5521 gccggagcca gcaccgccat cgggctgttc atggcgaagt acacgcccgg atcgatgata
     5581 caggcggaaa ccagtgccat aatcgccacg aaggattcca ttaacattcc accgtagccg
     5641 ataaagcacg cctgcccttc gttcgccagc atcttcggcg tggtgccgga agagatcagc
     5701 gcatggaagc ctgacaccgc gccacaggcg atggtgataa acaggaacgg gaacagatta
     5761 ccggtccata ccgggccagt gccatcgaca aatttggtca gcgcaggcat ggtcagcgtc
     5821 ggacgcataa tcaaaatgcc taccgccaga ccaacgatag tcccgatttt caggaaggta
     5881 gagaggtagt cacgcggggc cagcagtaac cacaccggca gcaccgccgc cacaaaaccg
     5941 tagcccacca gcatccaggt cagctgcacg ccggtaaagt caaagtacgg tgcccaggtc
     6001 gggctttctg ccacccagcc gccagagata atggcaaaaa taaggaacac caggccaatc
     6061 actgacactt cgccaatacg ccccggacgc agatagcgca ggtagattcc cataaaaatc
     6121 gccagcggaa tggtgaacgc aacggtatac gttccccacg ggctatgagt cagggctttc
     6181 accacgatca tcgccagtac tgcaaggata atgaccatga tcataaagca ggctaccagc
     6241 gcaatcaccc cggcagttgg ccccatctct tctttgacca gctcacccag cgagcgaccg
     6301 tcacggcgcg tagaaacaaa cagcaccatg aaatcctgca ccgcaccggc gagaaccacc
     6361 ccggcgagca gccagatcat ccccggcagg tagcccattt gcgctgccag caccggcccc
     6421 accagcggcc ctgctccggc aatggccgca aaatggtgac cgaacagcac tttcttgtcc
     6481 gtcggcacat agtccagccc gtcgttatgg cgcaccgctg gcgtcatacg cgtcgggtca
     6541 accgccagca catttttggc gatatacagc ccataaaaac ggtaagcgat cagatagata
     6601 cagaccgacg ccaccacaat ccacagcgcg ttgatctgtt ccccacgatt taaagcaatg
     6661 tatcccagag caaatgctcc cattacagag agcactgtcc agacgaggta tttccctgat
     6721 ttgttcatag ttgttatccg tgtgcgtgtc catagagatg ttacattttg tttctataac
     6781 atctttgtac gatttaacaa cacggtaacc atgtaaatcg gagttgaaac caatatttaa
     6841 cttaacaatg ttaactcgat cactccgtta accgagtttt cctgcaatct catttaattc
     6901 tgtcgggctc atcccaaaac tgccgtaccc agccgacaag tgcagcaacg ccgcccctgt
     6961 tcatcgaaaa cgatgatttc ccagctctga ttttgccgcc caagatgcag cggctggcag
     7021 acgccgcgta ccttgccttc agacatcggg cgatgatggg tggcgttaag ttccgtaccc
     7081 accacacact gcccgtcacg ggtcatcata aatccggcca tcgatcccag cgtttcagcc
     7141 agcgccgccg acgcgccgcc atgcagcagg ccaaacggct gatgagtacg ggtatcaacc
     7201 ggcatttcgg cttccagcac atcatctccc agacgggtat acacaattcc cagatgcgcc
     7261 accattgtgt tatcgctggt ggcgttcagt tcgtcgagcg ttaaatggcg tttccagatc
     7321 atgcttatgc ccccagcgtt gagccgccat cgaccacaat atcctgtagg gtgatatggc
     7381 tggcgaggtc agaggcgagg aacaaaatcg tgttggcgat ctcttgtgga cgggcgattt
     7441 tccccagcgg aatgccgagt ttaaactgct cgccaaagcc gcgaatacgc tgttcttcgg
     7501 cgtcatcgct cacccacagc gtgcgttgca tatcggtgtc ggtggagcca ggcgaaacca
     7561 cattacagcg cacgccgcta accgccagtt ccagcccgac gctcaacgcc aggcttttca
     7621 gcgccgcttt cgatgcgcca taagcactca taccaatacg cggcgtgtgt gcggcgtcgg
     7681 acgccacagt gacaatcgcc ccgccccgct gacggcgaaa ctggttcatg gtttgctgaa
     7741 acaggttaaa cgcaccgccg acgttaaccg caaaagtctg ctgccagtcc tctttactga
     7801 gctgatcggt cgcgcccatg cgcaaaattc ccgccgcatt gaccagcacg tccagtcgct
     7861 ccgtttcagc taacagtcgc tgacatacct gcgcgacctg cgcagcatcg gcaacatcca
     7921 tcacttcggt cgcaaaggga tattgctcct gagcgaacgc ttgatcaaaa ccggtaactt
     7981 tcgctcccgc ttcaacaaac gccagcgccg tggcgtagcc gataccttta cctgcgccgg
     8041 ttacccagac atttttaccg ctgaaatcca ttatttcacc tcgcgggaga gtagcttcca
     8101 ccaggcgtcg atggtcgggt ttttcgccag catgacaaag tcgatatcgc catgcacttt
     8161 gcgccagcgc gccgccagcg ccatcatgcg caccgaatcc agaccgtagt cgatcaggtt
     8221 gtcgtcatcg aacggttcat cggactcgtc cagcaacggc aggataacct cacgcagcgc
     8281 cgctttcctg gcggggacag gtgctggcag taatccttca gtcatcacca cccggccaga
     8341 ccgcccggcc acatatttca gcgacatcaa atgctcgtca cggctgaaat cggccagcgc
     8401 atccgccacc ataaacggtt taatatcgcg cataaatgcg tcggttgcgg tggtcataca
     8461 gccaatgtgg gcatataccc cggtaataat cagctggtta cgtccactct ctttgagcat
     8521 ttgctccagc ggagaacgat gaaacgcgct gtagcgccac ttcaccagca ccgtgtcgtc
     8581 ggcatctggc gtcaggcgat ccaccacctt ttgctgttcc ggcgaacggg tcaggcccgg
     8641 cccccacata tcattcaaca gcgcccgatc ttcatcgctc tgctctttcg gctgggcggt
     8701 gtaataaacc gggatattgt gctgtttgca gtagtcgcgc agcgcagcaa tattcgcgat
     8761 cacctgctcc atcatcgggc agttctcgcc ccagaagctg acaaaatagt cctgcatatc
     8821 atggattaac aacgcggcac gttgcggttc aaaggcccag tcaactttat tctgcggaat
     8881 atcgtgagac tccggcagtg cgtaagcctg taattttgga atagccatcg tgttctctcc
     8941 ttcaggctga tgcgcgtgac gccagccact gacgtaattg ttttttatcg actttcccga
     9001 ccgccgtcag cggaagtgaa tccacacact ccacgcgatc cggtaattta aattcggcaa
     9061 taccctgttc acgcaggaaa cgacgcacct gcaccgcgcg cagcggctct tttaccacca
     9121 gataagcaca gcttttttcg cccatcagct catcttccat gctcaccagt gcggcataga
     9181 tcaccgccgg atggcgcagc agcaggtttt cgatctcttc ggcagcgatc ttctcgccgc
     9241 cacggttgat ctgatctttc tcgcgcccct gcacggtgat gtaaccctct ggatcaatag
     9301 agatcagatc gccggaacag taaaaaccgt tggcatcaaa ggcactggca ttgtgctgcg
     9361 gacttttgta ataaccgcgg aaggtgtacg gcccgcgcgt catcaggcgt ccaacttccc
     9421 cttgcggcag tggatttcct tcagcatcag caacccatac ttcatcgtcc ggacacattg
     9481 ggtaaccctg ggtatggata attttctccg cgctatcatc aagtcgggtg tagttcacca
     9541 gcccttccgc catgccaaac acctgctgca actggcagcc aatctcagcg gaaatacgcg
     9601 ccgcaagggt ggcagaaaga cgtgcgccgc cgacctgtaa cagtttcagc gaggcaagct
     9661 gcgcccggct ttcgccttca gccagcgcct gcaaccacag gctaactgcc ggcggcacca
     9721 gcgcggtaac gttaacctga tgttttgcaa tcaacgggaa gcaaagcgtg gcgctgggat
     9781 cggccgccag aacaaccgtt cctccggcaa gaaagacgcc cagcgatcct ggcgaactca
     9841 tagcgtagtt atgagccgcc gggatcgcgc acaggtagcg tgtctgttgt gtgaactgac
     9901 aaatctcgac gctacgacgc acgctgtagt agtagtcgtt atgagtgcgc gggatcagct
     9961 tcggtgtacc ggtggtgccg ccggaaagct ggaaataggc cacttcatca gcaggtgatg
    10021 gcgtagcagt aaaaccgtct gccggatggt taatcgcatc ctgcaagtta tgctcaccgc
    10081 tgtcgttgag cagttgcacc acgcgaatgg aggaatgttc tgtgacgaaa gtattgagga
    10141 aatcatcccc gctaaacagc gcatgttggc gatcggcaat cagtaacgcc ggttcaatct
    10201 ggctggcata ggcgttcagt tcactacgct gatggctgaa caacgccagc accggcgcaa
    10261 cgcccagttt cagcagtgcg aaaaaggtaa tgtaaagttc agcgacgtta cccagttgta
    10321 ccagcgcggt ttcaccgggt ttaatgccct gacggcgtaa actacacgcg aggttatccg
    10381 ccgcctgatt cagctcccgg taactcaact gtcgctcgcc gtcgataacc gcgatgctgt
    10441 cactcgcagc gtggcgagtc agaatgtcgg tcagcggcaa atcctgccag tagccttttt
    10501 cccgatagcg acgggcaaac tcttccggcc agcgggtgaa tggaatgctc atcctcgctc
    10561 cttaatgcaa tccaaaaacg ttcaacatgg tagaaagttt gacgcctgtt tcgcgccact
    10621 cacccaacgg tgacgacgca ggcacaatcc ccgctccggc aaacagacgc acctgatttt
    10681 cccgcagctt cgcgcagcgg atggtcacca cccattcgcc gttaccttcg ctgtcacacc
    10741 aacccacaat gccgccaaac agttcgcggt caaacggctc cagttcagca ataacctggg
    10801 tcgcggcctg atgcgggaaa ccgctcagcg cgggggtcgg atgcagcaga caggccagag
    10861 tcagtgcgtt ttcttgcgaa ttcgctttac cttcaaaggg agttgcgaga tgccacagcg
    10921 tcggcgtggt aatcaattgt ggagaggagg gaacgtgtaa ctcactactg cgttcgcgca
    10981 gtacctcttt catcgcctga gtcaccagtt catgttcatg gcgatctttt tctgacgcca
    11041 gcagacgatt acccgcttcg cgatcgagca cttcatccgg ctgacgacgc gcggaaccgg
    11101 ctaacggaat ggagctaaaa cgctcgccgt ctttacgtag cagcagttcc gggctggccc
    11161 ccagcaggac gccaccatca gccagcggga catggaagtt gtaactaacc gggttttgcg
    11221 caatcaaccg ttccagcaat acgccactat caatggcggc gtcagtggtg atatcaatca
    11281 atcgtgacaa caccactttg tcgacctgcg gcgtggcggt aagtgcggcg gcgcgggcaa
    11341 ccatctgttc aaacgtggtt tgttcaggaa ttgcctggcg ttccaccacg ttcagcgact
    11401 ggctgcgggt aaaacagtgg gctgaggtct gtttttcctg acgggagaac gactgccagg
    11461 attcgggaat atacagcgac gaaggctgac gtggatcgaa gggaatcgcc ccgaccatca
    11521 ccggattttt gatgccctgc gctttggcat cggcaaacag cgcggcgagt ttttgctgga
    11581 agggactgtc gggcgaatcc ccgttcacag ccggttcatc gaagcgggcg aaacatcctg
    11641 acgtcgtaaa actgcggtac ggcgacataa agaaaaagcg attgggcgca agtgttgcca
    11701 tggtctgctg tacttcctca gccagtgacg tatccatatc atcctccaca aaatgataaa
    11761 ggctttaata atgattatca tttatatttt cggtcgctaa cctaaaagca aacgcgcact
    11821 atgtcaactc ttgaggtaac gcaatctgca actcggggtt gcatctggtt gtggttacaa
    11881 tgaaaatgag aagcattatt gatgaattcg cataagcgca atgtgatgtc ctgcgccgtt
    11941 ttgccccctc tcccttccag ggtgagggct ggggtgaggg ttaatgttcg caccagtgct
    12001 ggctgttccc ctcaccctaa ccctctcccc aaaggggcga ggggacggat tgtgcgcttt
    12061 gtcgaatttg tcattacgcc cttaacctta ttaataacag gaagctgact tgtgagactc
    12121 gccccgctct accgcaacgc ccttctatta acaggacttt tgctttcagg aatagccgta
    12181 gttcaggccg ccgactggcc gcgtcagatt actgacagcc ggggcactca taccctggaa
    12241 agccagccgc agcgtattgt ttccaccagc gtcaccctga ccggatcact gctggcgatt
    12301 gatgctccgg tgatcgccag cggcgcgacc acgccgaata accgcgtcgc agatgaccag
    12361 ggctttttac gccagtggag caaggtggcg aaagaacgca aactgcaacg gctctatatc
    12421 ggcgaaccga gcgccgaagc cgttgccgcg caaatgccgg atctgatttt aattagcgca
    12481 accggcgggg attcggcgct ggcgctgtat gatcagcttt ccaccatcgc cccgacatta
    12541 atcatcaatt acgacgacaa aagctggcag tcgctgttaa cgcagcttgg cgaaattacc
    12601 gggcatgaga aacaagcggc agagcggatt gcacagtttg ataagcaact ggcagcggcg
    12661 aaagagcaaa tcaaattacc gccgcagccg gtcactgcca ttgtctatac cgccgctgca
    12721 cacagtgcca atctctggac gccggaatca gcacaagggc agatgctgga acaactcggc
    12781 tttacgctgg cgaagttgcc cgcagggtta aacgccagcc aaagccaggg caaacgccat
    12841 gacatcattc agcttggtgg ggaaaatctg gctgctgggt taaacggtga atcgctattc
    12901 ctgttcgccg gtgatcagaa agacgccgat gcgatttatg ccaatccact gctcgcgcac
    12961 ctgcctgcag tacaaaacaa gcaggtttat acgctgggaa ccgagacgtt ccgcctggat
    13021 tactacagcg ccatgcaagt gctggacaga cttaattcac tgttaaaaaa agattgataa
    13081 aacatacaat tagagcacta ataatcccct cgcccctttg gggagagggt tagggtgagg
    13141 ggaaaatgca cctttagcta actgtcagac gctgtcacct gcggcggcgt ctggcgaaaa
    13201 cgtcgcaact ccaccagcac cagcaataac aacacgccga taatcaacaa accaaaaccg
    13261 ctcgcgcttg cggaagcaac cggtgtcatc atcgcgccca aaccacccaa cagcgccgcg
    13321 cctatggcat cgcccgtcac gttctgcgcc gtccacaaac cgttaatccg ccctaacatc
    13381 gcttccgggg tttgcgtttg cagcattgta tattgcagca acgaactaac cgcgctcagc
    13441 cagccgaaca gcgccagaca aaccacgcct aaaatccaca tcggcatcag gccaaacaga
    13501 ccaatggcga ggaacgatcc cagcgtggag agcagcatca ataaccctgg tcgcgcacta
    13561 tgtgccagct tcccgctggt taacgcacca atagccgcac cgagcgggat cgccgcatag
    13621 agaaaaccaa tctgtgccgc cgacatctgc cagttgtcag ccagcgccgg atacagtacc
    13681 cgcaccgcgc tcgccatcgt caataaacca cccagcaacg caatccctcc taccagcggg
    13741 ctggcgagca gaaaacgaaa tcctgccagt aatgatttca acggatgctc gcgcggctgc
    13801 ggtggcggtg gcaacgccgg aaggcttaac aacggtagca aggtaataaa cgtgcccgcc
    13861 gccgccagcc cgtagttcca ggctacgccg ccggtcgcca gcaataaacc gccaatcatg
    13921 ggcgaaatca ccgaccccag acgcacggtc aacatggtga tcgccccggc ctgcattaag
    13981 ttttcacgcc ctaccagtgc aggtgttgcc gccagtagcg ccgtaacacc aagtgatgcg
    14041 aaaaaaccat cccataaacc aagtaaatag attgccagca atgacggctc cggcagcagc
    14101 gcgttcaggc acagtccaat gaagccaatg ccacaggtgc cgcgcgccag caaaatcact
    14161 tttttgcgct catagcgatc cgccagcaca ccgccaacca tcaggccaac aaacatcgcg
    14221 ccgccggtca gcgtcaccga aagccccacc tgccaggtag aatgcgtcat catctggatc
    14281 tgcaccggca ccgcgacgcc gagcaaaccc agagacacaa ttgagataaa acgagcgagg
    14341 aatactgcgc gaaacgccgg gtgcgttttc aacaggctga ggttaagcag ccaggattgt
    14401 ttattcatta caatgccttg ccatcaaata ttttaaatac ctattcttag gctgcacatg
    14461 ctaacatatc caaataagat cgataacgat aattaatttc attatcatgg aagttcgtat
    14521 gtctggttct gttgccgtga cacgcgccat tgccgtgccc ggattgctgt tattactgat
    14581 tatcgcgacg gcattaagcc tgctcattgg ggcaaaatca ctccccgcgt cagtagtgct
    14641 ggaggccttc tcaggcacct gccagagcgc cgactgcacc atcgtgctcg acgcgcgact
    14701 gccgcgcacc cttgccggtt tacttgcagg cggtgcgctt gggcttgctg gggcgttaat
    14761 gcaaaccctc acccgaaatc cacttgccga ccccggcttg cttggcgtga acgccggagc
    14821 cagctttgcc attgtgctgg gtgcggcgct gtttggttac tcatccgcgc aggaacaact
    14881 ggcgatggcc ttcgccgggg cgctggtggc ctcattgatt gttgccttta tcggcagtca
    14941 gggcggcggg cagttaagtc cggtgcgttt aaccctggcg ggcgtggcgc tggcggcggt
    15001 gctggaaggg ctgaccagcg gcatcgccct gcttaatcct gacgtctacg atcaactgcg
    15061 tttctggcaa gccggttcgc tggatattcg caatctacat accttaaaag tggtgctgat
    15121 cccggttctg atcgccggag caactgcgct attactgagt cgcgcgctga acagtttgag
    15181 cctcggcagt gacaccgcga cggcgctggg cagtcgcgtg gcgcgcacac agttgattgg
    15241 tctgctggcg attaccgtgc tttgtggtag tgcgacggcg gtagttggcc cgattgcctt
    15301 tatcggcctg atgatgccgc acatggcgcg ctggctggta ggtgccgatc atcgctggtc
    15361 gctgcccgtc acgctacttg ctacccctgc cctgctgctg tttgccgata tcatcgggcg
    15421 ggtgattgtg cccggcgaac tgcgcgtttc tgtggtcagt gcgtttattg gtgcaccggt
    15481 gctgatcttc ctcgtgcgac gtaaaacgcg aggtggtgca tgatttacgt ctctcgccga
    15541 ttactcatca cctgtttgct gctgatttcc gcctgtgtgg ttgcaggtat ctggggatta
    15601 cgcagcggtg ccgtcacgct ggaaatctcg caggtattcg ccgcgttgat gggcgatgcg
    15661 ccgcgcagta tgacgatggt ggtcaccgaa tggcgtttac cacgcgtgct gatggcgctg
    15721 ttgattggcg cggcgctggg cgtcagcggc gcgatttttc agtcgctgat gcgtaacccg
    15781 ctcggcagcc ctgacgtaat gggctttaac accggggcgt ggagcggcgt gctggtggcg
    15841 atggtgctgt ttggtcagga cctgacggct atcgcgctgg cagcaatggt gggcggcatt
    15901 gtcacttctc tgttggtctg gctgctcgcc tggcgtaacg gtatcgacac ctttcggttg
    15961 attattatcg gcatcggcgt tcgcgccatg ctggtggcct ttaatacttg gctgttgctg
    16021 aaagcgtctt tagaaacggc gctaacagcg ggtttgtgga atgccggatc gctcaatggc
    16081 ctgacgtggg caaaaacttc gccttccgca cccatcatca tattgatgct cattgccgcc
    16141 gccttgctgg tgcggcgaat gcgcttgctg gagatgggcg atgataccgc ctgcgcgctg
    16201 ggcgtcagcg tcgagcgttc gcgtctgtta atgatgctgg ttgcagtggt gcttaccgct
    16261 gcggcaacgg cgcttgccgg gccgatttcc tttattgctt tagtcgcgcc gcacattgcc
    16321 cgacgcatta gcggcaccgc tcgctggggg ctgacccagg cggcgctgtg cggagcgctg
    16381 ttactgctgg cggccgatct ctgcgcccaa caactgttta tgccgtatca acttccggtt
    16441 ggcgtcgtta ccgtcagcct cggcggtatt taccttatcg tcttgttaat tcaggagtct
    16501 cgcaaaaaat gaccgaatca gtagcccgtt tgcgcggcga acagttaacc ctgggatatg
    16561 gcaaatatac cgttgcggaa aatctgactg tagaaattcc tgatggtcac ttcacggcaa
    16621 ttatcggacc aaatggctgt ggtaaatcca cgttactgcg taccttaagc cgcctgatga
    16681 cgcctgctca cgggcatgtc tggctggatg gcgagcacat tcaacattac gccagtaaag
    16741 aagttgcacg ccggattggt ctgttggcgc aaaatgctac cacgccgggc gatatcaccg
    16801 tgcaggagct ggtggcgcgt ggacgttatc cgcatcaacc gctgtttacc cgctggcgca
    16861 aagaggatga agaagcggta acgaaagcga tgcaggccac gagaataact catctggcag
    16921 atcaaagcgt ggataccctt tctggcggac aacgccagcg agcgtggatc gcgatggtgc
    16981 tagcccagga aacggcaatt atgctgctcg acgaacccac gacctggctg gatatcagtc
    17041 atcagattga tctactggaa ctgttaagcg aactgaaccg cgagaaaggc tataccctgg
    17101 cggcggtgct gcacgatctt aatcaggcct gtcgttacgc cagccatttg attgcattgc
    17161 gggaagggaa aattgttgct cagggagcgc cgaaggagat tgtcactgct gaactgattg
    17221 agcgcattta tggtctgcga tgcatgatca ttgacgatcc ggtagccgga acgccgcttg
    17281 tggtgccgct cggacgaacg gcaccttcaa ccgcaaatag ttaaactaag tggtctgcca
    17341 tcatggcatc ctgttttctg gatgccatcg catagcgcaa taacacgcta ccacaagcca
    17401 ccatcccgcc gatcagcgcg gaaaggatca caataatcgc cttacccgga ccgtcttttt
    17461 tcactggcaa tgacgggctt aactgatatt taaacggcgt aaaattcaca tcgttgacat
    17521 ttgcttttgt taattgctcg acaagatact gccgattacg taattcaccg ttcagttccg
    17581 caacgtcagt aaccgctttt tcaatttcca gtttgcgttc aataccgtct gcgccgagag
    17641 aaatagagaa atcggggtca tctttaacgg cctgaccatt actgtatacg ggctttttaa
    17701 ttcctgccgc gttggcaatg tcgagtgaat aattgaggcg ctgaatgttt gcatcaagtt
    17761 gatttttcgt tttaatgcga tcctgagcca gtttttcttt ttcaaactgg gttttgatct
    17821 ccagtttatt acggacgttt tctatcgact ctttcaccac caacgcagag atataatcga
    17881 tatacccgct caaaacggtc tgcgcctctt tactggttgg cgcggtaaaa cttagcgtcc
    17941 aggaggtata cagtgacggt tcatcttttt tcttactggc attgtcatca accgctttca
    18001 ttttttcgct caatgcaaca atagcgcgat gcaaatccag ttcatcgatt ttcgcctctt
    18061 ttaattggtc catcacataa ggtgatgaac gcaggtactc ttccagcaag ctaacagact
    18121 gaaacttctt gataaacagg ttaaatgcac cgccacggtc gatattaaca tcgagatcaa
    18181 gaacgcggag tttggtaaag gttttctcca ggtcctgcca ctgaatggct tcggcgggag
    18241 taataacggc tgagctggtc catttttgcg gcaggatgaa agagatcagt aaacctgcgc
    18301 aggcaaatgc aaaaacgacc gccatgaccg tttttttggc ccgccataaa acttcgatta
    18361 gattaagtaa atcaatttca ttattactgg gcgacgccag aggatactca gaaaaatgag
    18421 cgtcacttcc ctgtttaata ttcaatgatg acatggggta taacctgaaa atgagtccaa
    18481 taggggaaaa tctccacggc gctcacttta ccagttggtg agaattatcc gaagctgaag
    18541 tttgtaaatt ccttccactg aatgcaatat tcactctatt taagagaaat attcatcaaa
    18601 cctataaatc aaacaaataa aaaacccata attattttct gtaattatgg gtttatagat
    18661 aatattaatt tacctgttta gcgttgcgcg aataatcggc ccaattttta caaacgcccc
    18721 tggagagata atatccacat gcgcacaatc ctgacgatag atatccagct ccgcaatcca
    18781 cggcgaccag gcgcgttcag gactcatacc ttcctgaagc gtgcgttcag caacaaacaa
    18841 cgtcgcttta ccgtcaaacg gtacgctatg agcagtcgtc agcaggcgca cagcatcagc
    18901 gtagttgcct tcaatggtgg taaacaactc cgttgaagta cttccctgct gtgccgccag
    18961 gaaggcctcg cgctcgcggt taatctccgc cagcacttcc gggtccagac cattagcttc
    19021 tttttcctgc cagttttgcg tttctggcgg ccaggtatcc agcaagccaa gaaatgccac
    19081 ctgttcgcca cgggcacgca gtcgcgccgc aataccctgc gccagcgtac cgccaaggga
    19141 ataccccagc aggtaataag ggccgtgcgg ttgttgctca agtaacgttg ccagatgcgc
    19201 ttcgcagact tcatccaggt ttgtcgctgt ctgcatgggt ccatgagggc gcggcgactg
    19261 aatgccaata atcgaccatt gtggatcgag ataacgcgag agtacgctaa actgccaggc
    19321 aaaaccggac gccggatgga aacaaaacag cgtcgggcca ttaccttcac gcaacggcag
    19381 aatggtttcg aatcccatgc gccgggagct gtcctcttca ccatcaataa tcgttgccag
    19441 tttggcgacg gttgacgcga ccatcacctg ccccggcgtc acctgacgag caaactgtcg
    19501 acttaactgc gctgccagtt tcattgccag tagcgaatga ccgcccagcg caaagaaatc
    19561 agcatcggca tcctgcacgt cacaacccag caacgacgcg aatgccgcgg cgataatcgt
    19621 ttcactgccc gctttcggtg cacgtcccga cgcttgtgtc ttcagttcag gcaacggtaa
    19681 ggctttgcga tccagcttgc cgttggcgct aagaggtaac tgtggaagtt gcagcagaac
    19741 caccggtacc atatgtggtg gcaatgtttc acgaagctgc gcctgcaatg cgctggtatc
    19801 caacggcagg cccgattgcg acaccagata gcccaccaac tgacgcgcat caccaccagt
    19861 ggctgccgcc tggttaatca cacaggcgtg ggtaacggct tgttcgacat ccggcagcgc
    19921 ctgcatcacg cgatcgattt cgcccagttc gatacgctgc ccgcgaattt ttagctgatc
    19981 gtcactgcgc ccgaggtact ccaccgcgcc gttatccagc cagcgggcaa cgtctccggt
    20041 acggtacatc cgttcgccag gagcaaaagg atcggcaata aagcggctgg cggtcagatc
    20101 ggggcgtccg agatagccct gcgccagttg aatgccggta agatagagat cacccgccac
    20161 acccggcggc accggatgca tcatcgcatc aagaatacgc agccccatgt tccacaccgg
    20221 ataaccaatc ggcacactgc tgccgcgcac ctgtgccagt tcctcgccaa aagccggata
    20281 ccagctgaca tctaccgccg cttccgtcgg gccatacaga ttatgtaacg gcgcaccggt
    20341 taactgttgc cattcacggc ataaatcagc cggtaaggct tcaccactac agaaaacctg
    20401 tttcaacgtc gcgcaactct ggcgagcggt ttgcggagtc agcgaggcaa caaatgccgc
    20461 cagcatcgac ggcacaaagt gcgtggtcgt tacgccatat tcggcaaaga attgctgcat
    20521 agcgagcggg tcacggtgcg cttccggttc agccatcacc agttttgccc ctgcgataaa
    20581 cggccagaaa aactcccaca ccgagacatc aaaactgcac ggcgtttttt gggcaacgac
    20641 atcttcgcct gtaagcgggt agtgattttg catccataac aggcggttaa cgatagccgt
    20701 ctgcccgacc attacccctt tcggcctgcc ggtggagcca gaggtaaaga tgatataagc
    20761 cgtgtggtgc ggttgtgaaa gttgcagcgg cgcactgccc tggggggtaa gcggggcgtt
    20821 atagcaaagg cttgttaaat tgggaacatc gctaaagcgc ggcagttgat cgtcggtggt
    20881 aattaacagc gacggacgcg catcttccag catcattttc aggcgatcgt ccggatagcc
    20941 ggtatccagc ggtagccagg ccgcgccagc ttcaactatt gcatggagtg ccagggtcaa
    21001 aaagaccgag cgcggtagtg ccaccgccac gctgtcccct ggtttaacgc cgcgctcacg
    21061 cagcagattc gccagcgcca ccacctgctc gcgcatttcc cgatagctga acaggtaacg
    21121 cgcatctgcc agcgccggag catccggtgt ttttgccgct tgttctgcca ccagcgcgct
    21181 cagcgtggtt tctggaatct caacctgagt ggcgttgatc tgcgccagct gcgcatactc
    21241 acctggcagc ataatatcga catcgccgca caacagcgcc ggatccgcgg cgaactgggc
    21301 aatcagcatt ttcaggcgtt cagcatgctg gattaacgtt ggctcatcgt aacgctgttt
    21361 attggcgagg atctcaatac tcaaatcacc gtgttcatcc gggaacaggg ccagttcaag
    21421 gtcattaacc ggaccggttg ccagggtatg ggtttgcgcc tgaacaccag gaatatccag
    21481 ttggtaatca aataccttaa tattgagtac cggaccaaac agcggttcat cacctgccgc
    21541 tcgcccgctg tcacggacaa tttgttcggc atcgtaacgt tgatggcgac gcattttttt
    21601 cagttgagcc gccagtcggg ttgccagttc cggcagcgtt tcttgcgccg caatgtgaat
    21661 acccaacggt aaaacgttga gcacgggtcc gctagccgtc agcgccgccg agcccagtcg
    21721 acgcataaag ataaatccgg cggcatagtc catgcgattg cacaatcgcc ccagccacaa
    21781 ggctgccagc gcaagggcta aatcggtacg ctgcacacct gaaagttgcg tagccagctg
    21841 gcggaattcc ccgtcggtaa attccagttt caggcgcaga atatctgccg aagcgctgcg
    21901 ccccggtaaa ggtgccggag aaagtgacgc gggcggcggc agttgacgac gctgttctgc
    21961 ccagaatgcc gcatcacgct gccaggcttc gctttcgcgg tattgctggt actcttccac
    22021 tacatcagcg aaaggcgtaa acggcgaagc aggcgttggt tcgccacgca gccatgtgca
    22081 gtaaatattg gcgatctggc gagtaatggc cgggaaactg aagccatcga ccagcaaatg
    22141 gtgataacgc tgataccagt accagcggtt atccgcaaca tgtatcagct gatgaaagac
    22201 cagtggttta ccgctatcga cgcgcaaatc ttgttgcaaa tccgccagca ttaatgcctg
    22261 cgcagtaccg tgcggatcaa tattggttcg caggtcgata atttctggct gttcgaatat
    22321 cagcgcatca tcgacccact gccagacttc gccgttatct tccgtaaaac gcatccgcaa
    22381 cgtatctgct tgcgctagtc cggcaaccac cgcgcgggcc agtaatggcg catcaacatc
    22441 tccagttaac tcaacgtaat gcgccacgct ccaggcggag ggtaaatccg acagtttttc
    22501 tgccatccag atgccgggct gtgcggcgac caaaggtaaa tgctggctca ttgtgcctcc
    22561 tgcaactggg tgaaattggc cggtgtcaga gtacgccagt ggacttccag ccactgctgg
    22621 caggacgcct gtgactgcgg ctgacacaca atgtcccagc ctgccgataa gaggcattgt
    22681 tgcggccaca gactgaattg cccctgcgca ttgcgcaaaa tgtaaaacgc tccctgcgga
    22741 tcatcgaagg gattactgaa tgccatactc aactcctgtc atggaaaagt ggttgccaga
    22801 ggtcgattaa cccctgcatc aagccaccgc gccaacaaag cgcatcatgt ccgccgtcaa
    22861 cctgacgcca gaaaatggat tcttttatag ggtgcaattg cgcatacagc gcctgattgg
    22921 cccgcatgat catcggctcg cgaatacccg cttccagcac aatgcgcaga ccttcggcgc
    22981 taacttcacc agcttttagc ttttcaagta acacgccctc ttgctgcccg tcccgatgcg
    23041 gccaccagta cgatcctgac tggcttaata cacagccaaa gcgttcaggc cagtgcagtc
    23101 cggcatacag cgcggacagc ccaccaaaac tctgcccggc gaccacggtg cgatcggcgc
    23161 gatcgctaaa aggggcaata gctttcacca ggggtaataa ctcttgctgt actgccagcc
    23221 agaaatccgc attacacggc agttcgtggg cgcggtgcgt ggtgtcgata gcgtcgatca
    23281 acacatacac ggcgggagga agttgctgac gatgggtcag cgaagtcagc acaggccaga
    23341 cgggcatact ttgcgcccaa aattcgccat cgagcaaaac tgccagcgga cgttcttctg
    23401 ctgttacatc gccggtggta aaaatccata cacgacgtga atttttcaac cgttcacttt
    23461 tccagataat ttctttggct gctatttctg gcacttgcgg acaatcccat cccggttgca
    23521 gaggcgcttg cggcatttcg agtgcagaaa cagcgtgccc tcgcccgcct ttccagcttt
    23581 gtggattcag cggatcggct attgcctggg gtaatagttt tcgccagcct tcacgtaatt
    23641 cgaggcgatc gggggatggt gcagaaaaaa tgtcatcgcg ttcggtggga ataaagcagt
    23701 agctgccgcg ccagttggca ttgagttgtg tcgtccactg ccagacatca gtacctgcaa
    23761 ttcgctgcat cgactggggc tggctgttct gatgatgatc ggtcacacca gtgatgtaga
    23821 tccatacgcg ctttatcgtc gagtattctt cagaaccttg gggatcacgc caccagaaag
    23881 tgacctcaaa catttcgtca ttcagacgct gccattccgg gccatgtttc gactgccacc
    23941 agctctcact tcctactttt aacgccgtca caccataacc ccatgtttac tgtgcaattt
    24001 ttcattgatt gcagaaatat attgataata ttattgataa ccatttgcat ttgcaatagc
    24061 gtaatggcgc gccgtgggaa gcgcggacat ttattaacca actgcactgc gtgtctttca
    24121 ggatcaaagg ttttcgcggt agcgggatgc gtcgtgttga ggacgaccat gcccgacagt
    24181 tgcaattcgt ggcaaaaatg caggaataaa acaatgaaca agaagattca ttccctggcc
    24241 ttgttggtca atctggggat ttatggggta gcgcaggcac aagagccgac cgatactcct
    24301 gtttcacatg acgataccat tgtcgttacc gccgccgagc agaacttaca ggcgcctggc
    24361 gtttcgacca tcaccgcaga tgaaatccgc aaaaaccccg ttgcccgcga tgtgtcggag
    24421 atcatccgta ccatgccagg cgttaacctg accggtaact ccaccagtgg tcagcgtggg
    24481 aataaccgac agattgatat tcgcggtatg ggtccggaaa acacgctgat tttgattgac
    24541 ggcaagccgg taagcagccg taactcggtg cgtcagggct ggcgtggcga gcgcgatacc
    24601 cgtggtgata cctcctgggt gccacctgaa atgattgaac gtattgaagt tctgcgtggt
    24661 ccggcagctg cgcgttatgg caacggcgcg gcgggcggcg tggttaacat cattaccaaa
    24721 aaaggcagcg gtgagtggca cggctcctgg gacgcttatt tcaatgcacc agaacataaa
    24781 gaggaaggtg ccactaaacg caccaacttc agcctgaccg gtccgctggg cgacgaattc
    24841 agcttccgct tgtatggcaa cctcgacaaa acccaggctg acgcgtggga tatcaaccag
    24901 ggccatcagt ccgcgcgtgc cggaacgtat gccacgacgt taccagccgg gcgcgaaggg
    24961 gtgatcaaca aagatattaa tggcgtggtg cgctgggatt tcgcgccatt gcaatcgctg
    25021 gaactggaag caggttacag ccgccagggc aacctgtatg cgggcgacac ccagaacacc
    25081 aactccgatt cctatacccg ctcgaaatat ggcgatgaaa ccaaccgtct gtatcgccag
    25141 aactactcgc tgacctggaa cggcggctgg gataacggag tgaccaccag caactgggtg
    25201 cagtatgaac acacccgtaa ctcgcgtatt ccggaaggtc tggcaggcgg taccgaaggg
    25261 aaatttaacg aaaaagcgac acaggatttc gtcgatatcg atcttgatga cgtgatgctg
    25321 cacagcgaag ttaacctgcc gattgatttc ctcgttaacc aaacgctgac gctgggtacg
    25381 gagtggaatc agcaacggat gaaggacttg agttccaata cccaggcgct gaccggggcg
    25441 aacaccggcg gcgctattga tggtgtgagt gccaccgacc gtagcccgta ttcaaaagca
    25501 gaaattttct cgctgtttgc cgaaaacaac atggagctga ctgacagcac catcgtaacg
    25561 ccggggctgc gtttcgatca tcacagtatt gtcggcaata actggagccc ggcgctgaac
    25621 atatcgcaag gtttaggcga tgacttcacg ctcaaaatgg gcattgcccg tgcttataaa
    25681 gcaccgagcc tgtaccagac taacccgaac tacattctct acagtaaagg tcagggctgc
    25741 tatgccagcg cgggcggctg ctatcagcaa ggtaacgatg acctgaaagc agaaaccagc
    25801 atcaacaaag agattggtct ggagttcaaa cgcgacggct ggctggcggg cgtcacctgg
    25861 ttccgtaacg attatcgcaa taagattgaa gcgggctatg tggctgtagg gcaaaacgca
    25921 gtcggcaccg atctctatca gtgggataac gtgccgaaag cagtggttga aggtctggaa
    25981 ggatcgttaa acgtaccagt tagcgaaatg gtgatgtgga ccaataacat cacttatatg
    26041 ctgaagagtg aaaacaaaac cacgggcgac cgtttgtcga tcatcccgga gtacacgttg
    26101 aactcaacgc tgagctggca ggcgcgggaa gatttgtcga tgcaaacgac cttcacctgg
    26161 tacggtaagc agcagccgaa aaaatacaac tacaaaggtc agccagcggt tggcccggaa
    26221 accaaagaaa tcagtcctta cagcattgtg ggtctgagcg cgacctggga tgtgacgaag
    26281 aatgtcagtc tgaccggcgg cgtggacaac ctgttcgaca aacgtttgtg gcgtgcgggt
    26341 aatgcccaga ccacgggcga tctggcaggg gccaactata tcgcgggtgc cggggcgtat
    26401 acctataacg agccgggacg tacgtggtac atgagcgtaa acactcactt ctgatgctaa
    26461 cgtcagattg ttgacaaagt gcgcgtcgtt catgccggat gcggcgtgaa cgccttatcc
    26521 ggcctacaaa aatcttgcca attcaatata ttgcaggacc atgtaggcct gataagcgta
    26581 gcgcatcagg cagttttgcg tttgtcatca gtctcgaata tggtcgatat gaaaactacg
    26641 catacctccc tcccctttgc cggacatacg ctgcattttg ttgagttcga tccggcgaat
    26701 ttttgtgagc aggatttact ctggctgccg cactacgcac aattgcaaca cgctggacgt
    26761 aaacgtaaaa cagagcattt agccggacgg atcgctgctg tttatgcctt acgggaatat
    26821 ggctataaat gtgtgcccgc aatcggcgag ctacgccaac ctgtctggcc tgcggaggta
    26881 tacggcagta ttagccactg tgggactacg gcattagccg tggtatctcg tcaaccgatt
    26941 ggcattgata tagaagaaat tttttctgta caaaccgcaa gagaattgac agacaatatt
    27001 attacaccag cagaacacga gcgactcgcg gactgtggtt tagccttttc tctggcgctg
    27061 acactggcat tttccgccaa agagagcgca tttaaggcat cggagagaca aactgaagca
    27121 ggttttctgg attatcagat aattagctgg aataaacagc aggtcatcat tcatcgtgag
    27181 aatgagatgt ttgctgtgca ctggcagata aaagaaaaaa tagtcataac tctgtgccaa
    27241 cacgattaat tgacaacatc tggtacgatt cgcccgcagc catcactgac tgcgggcgaa
    27301 agtgtaaagc aggtgcctta ccactctgac ctgacaaccg gatatgcagg gattgctccc
    27361 cgcacaacgg ctacttcttc ggttcgtaag cgagaacagc cttaaactca atattacgtt
    27421 cctttacggt gaactcacac agcgagtctc cgaccagaag cgtaaatccc agcaccgtca
    27481 aacacagcac tatgactgcc acaagggcat atttcgtcag catgtttata ttgcctccat
    27541 gcgaagaagg gactaccatc cgagttgttg aggttcggag tggcagcccc aggattgata
    27601 gaaatatctc ctggggcttc atctttccag acctcagagt aaacctgaaa ccggaaagct
    27661 tcaggcaccc gccgttatct tacccttcct cagcaaaata tctgtttatt tatacagtaa
    27721 gtcctgtttc ggacttctgt tggcaacact cgttattgct cgtaaaacga aatcgcccgc
    27781 agccatcact gaccgcgggc gaaagtgtaa agcaggtgcc ctaccatcct gacctgacaa
    27841 ccggatatgc ggggattact ccccgcacaa cggctacttc ttcggttcgt aagcgagaac
    27901 agccttaaac tcaatattac gttccttcac cgtaaactca cacagcgagt ctccgaccag
    27961 aagcgtaaat cccagcaccg tcaaacacag cactatgact gccgcaaggg catatttcgt
    28021 cagcatgttt atattgcctc catgcgaaga agggactacc atccaaattg ttcgggtttg
    28081 gagtggcagc cccagggttg atagaaatat ctcctggggc ttcatctttc cagacctcag
    28141 agtaaacctg aaaccggaaa gcttcaggca cccgccgtta tcttaccctt cctcaacaaa
    28201 atggctgttt atttatacag taaatcctgt ttcggacttc tcactcctca tttaatcaac
    28261 tgaagctgag cccgtttttt ctgcatgcct caatcgtcaa aattcttcgc catcagccag
    28321 ctctgccata acttgccaca ttgaaaaagt agccaaattg cttcagtcac catcatcaaa
    28381 aaaagaaaca ttccagaact tccgcatctc gcccagccgc tccgacttct atactgaata
    28441 gaaaacgcca acataagaga aacctatgcc attacccgat tttcatgttt ctgaaccttt
    28501 taccctcggt attgaactgg aaatgcaggt ggttaatccg ccgggctatg acttaagcca
    28561 ggactcttca atgctgattg acgcggttaa aaataagatc acggccggag aggtaaagca
    28621 cgatatcacc gaaagtatgc tggagctggc gacggatgtt tgccgtgata tcaaccaggc
    28681 tgccgggcaa ttttcagcga tgcagaaagt cgtattgcag gcagccgcag accatcatct
    28741 ggaaatttgc ggcggtggca cacacccgtt tcagaaatgg cagcgtcagg aggtatgcga
    28801 taacgagcgc tatcaacgaa cgctggaaaa ctttggctat ctcatccagc aggcgaccgt
    28861 ttttggtcag catgtccatg ttggctgcgc cagtggcgat gacgccattt atttgctgca
    28921 cggcttgtca cggtttgtgc cgcactttat cgccctttcc gccgcgtcgc catatatgca
    28981 aggaacggat acgcgttttg cctcctcacg accgaatatt ttttccgcct ttcccgataa
    29041 tggcccgatg ccgtgggtca gtaactggca acaatttgaa gccctgtttc gctgtttgag
    29101 ttacaccacg atgatcgaca gcattaaaga tctgcactgg gatattcgcc ccagtcctca
    29161 ttttggcacg gtggaggttc gggtgatgga taccccgtta acccttagcc acgcggtaaa
    29221 tatggcggga ttaattcagg ctaccgccca ctggttactg acagaacgcc cgttcaaaca
    29281 tcaggagaaa gattacctgc tgtataaatt caaccgtttc caggcctgtc gctatgggct
    29341 ggaaggcgtc attaccgatc cgcacactgg cgatcgtcga ccgctaacgg aagacacctt
    29401 gcgattgctg gaaaaaatcg ccccttccgc acataaaatt ggtgcatcga gcgcgattga
    29461 ggccctgcat cgccaggtcg tcagcggtct gaatgaagcg cagctgatgc gcgatttcgt
    29521 cgccgatggt ggctcgctga ttgggctggt gaaaaagcat tgtgagatct gggccgatga
    29581 ctaagtaggg aattgcctta cagaagaata tccctgacaa tggtgtttta acttacattt
    29641 aacaatgtta tgaaacaccc tttagaaacc ttgactaccg cagcaggcat tttgctgatg
    29701 gctttcctct cttgcctgct gctgcccgcc cccgcactgg ggctgacgct ggcacaaaaa
    29761 ctggtgacca tgttccatct gatggatctt agctagcttt acactttatt gttttgtctg
    29821 tggtttttag tgctgggcgc tattgagtat tttgttctgc gctttatctg gcgacgctgg
    29881 ttctcgctgg cggattaaac gtcgattatc gccgtacttc gcggcatact ttgcttattc
    29941 tccttcgcct aaaggaacgt ttatggataa gcaatcactg cacgaaacgg cgaaacgcct
    30001 ggcccttgag ttaccctttg tcgagctttg ctggcctttt ggcccggagt tcgatgtttt
    30061 taaaattggc ggcaaaattt ttatgctgtc gtcggagctg cgcggcgtcc cctttatcaa
    30121 tctgaagtcc gatccacaaa aatccctgtt aaatcagcag atatacccga gcattaagcc
    30181 agggtatcac atgaataaaa agcactggat ttcggtgtat cccggcgagg aaatctccga
    30241 agcgttactt cgcgatctga tcaacgattc gtggaatctg gtggttgatg gtctggctaa
    30301 acgcgatcaa aaaagagtgc gtccaggcta aagcggaaat ctatagcgca tttttctcgc
    30361 ttaccatttc tcgttgaacc ttgtaatctg ctggcacgca aaattacttt cacatggagt
    30421 ctttatggat atcatttctg tcgccttaaa gcgtcattcc actaaggcat ttgatgccag
    30481 taaaaaactt accccggaac aggccgagca gatcaaaact ctcctgcaat acagcccatc
    30541 cagcaccaac tcccagccgt ggcattttat tgttgccagc acggaagaag gtaaagcgcg
    30601 tgttgccaaa tccgctgccg gtaattatgt gttcaacgaa cgtaaaatac ttgatgcctc
    30661 gcacgtcgtg gtgttctgtg caaaaaccgc gatggacgat gcctggctga agctggttgt
    30721 tgaccaggaa gatgctgatg gccgctttgc cacgccggaa gcgaaagccg cgaacgataa
    30781 aggtcgcaag ttcttcgctg atatgcaccg taaagatctg catgatgatg cagagtggat
    30841 ggcaaaacag gtttatctca acgtcggtaa cttcctgctc ggcgtggcgg ctctgggtct
    30901 ggacgcggta cccatcgaag gttttgacgc cgccatcctc gatgcagaat ttggtctgaa
    30961 agagaaaggc tacaccagtc tggtggttgt tccggtaggt catcacagcg ttgaagattt
    31021 taacgctacg ctgccgaaat ctcgtctgcc gcaaaacatc accttaaccg aagtgtaatt
    31081 ctctcttgcc gggcatctgc ccggctattt cccctcagat tctcctgatt tgcataaccc
    31141 tgtttcagcc gtcatcatag gctgctgttg tataaaggag acgttatgca ggatttaata
    31201 tcccaggttg aagatttagc gggtattgag atcgatcaca ccacctcgat ggtgatgatt
    31261 ttcggtatta tttttctgac cgccgtcgtg gtgcatatta ttttgcattg ggtggtactg
    31321 cggaccttcg aaaaacgagc catcgccagt tcacggcttt ggttgcaaat cataacccag
    31381 aataaactct tccaccgttt agcttttacc ctgcagggga ttatcgtcaa tattcaggcg
    31441 gtattctggc tgcaaaaagg caccgaagcg gcagatattc tgactacctg cgcgcagttg
    31501 tggatcatga tgtatgcgct gctttcagtc ttctcgttgc tggatgttat tttgaatctg
    31561 gcgcagaaat tcccggcggc atctcagtta ccgctgaaag ggatatttca ggggattaaa
    31621 ctgatcggcg cgatcctggt cggcattttg atgatctcgc tgctgattgg tcagtcgcca
    31681 gcgattctga tcagcggtct tggtgcaatg gctgccgtgc tgatgttggt atttaaagat
    31741 ccgattcttg gtctggtggc cggtattcag ctttccgcga acgatatgct gaaactgggc
    31801 gactggctgg agatgccgaa atacggcgca gatggcgcgg tgatcgatat tgggttaacc
    31861 accgtcaaag tgcgtaactg ggacaatacc attaccacta ttcccacctg gtctctggtt
    31921 tctgactcct ttaaaaactg gagcgggatg tcagcatctg gcgggcgacg tattaagcgc
    31981 agtatcagta ttgatgtcac cagtattcgt tttcttgatg aagacgaaat gcaacgtctg
    32041 aataaagcgc atttgttaaa gccttattta accagccgcc atcaggaaat taatgagtgg
    32101 aatcgccagc aaggttctac ggagtcggta ttaaacctgc gccgaatgac caatattgga
    32161 acctttcgtg cctatctgaa cgaatatctg cgtaaccatc cgcggattcg taaagatatg
    32221 accttaatgg tacgccaact ggctccaggt gataacggtt taccgctcga gatctacgcg
    32281 tttactaaca ccgtggtgtg gctggaatat gaaagcattc aggctgatat attcgatcac
    32341 atatttgcga ttgtcgaaga gtttggtctg cgacttcatc agtcgccaac cggcaatgat
    32401 attcgctctc tggcgggtgc atttaagcag taattaaaaa aaccgctctc atcgaatgga
    32461 tgagagcgga catcgttttg tcggatgtag cgtgaatgcc ttatttccga cgcagcgttt
    32521 taaatgccac aaacaggaat acaatccaca ccggcagcag gatcgctgac aagcgcatat
    32581 catccatcgt gcacatcagc agcaaaatca tgccgaggaa ggcaatgcag agatagtttc
    32641 cgaacggata gagcagcgcc ttaaactgtg tttcacgccc ctgacgtcgc atcgctgcac
    32701 gaaaacgcag atgcgccaga cagatcataa tccagttcaa cagcagcgtt gctaccacca
    32761 gcgccatcag cagaccaaac gctttttgcg gcagcagata gttgattaac accaccagcg
    32821 aagtgatcgc tccggaaagc atcagcgagt taatcggcac accgcgacgg ctgacgcgag
    32881 tcaaaaactt cggcgcatta ccctgcacag aaaggccaaa cagcatgcgg ctgttagagt
    32941 aaaccccgct gttatacact gacagcgatg ctaccagaat gacgaagttc agcgcagaag
    33001 ctaccacgtt gctgtcgaga ttatggaaaa tcatcacaaa cgggctactg ttggatttca
    33061 cttccaccca cggatagagc gccagtaaaa ccaccagtga accgatgtaa aacagcagga
    33121 tgcgatacac cacctgattt accgcttttg gaatgctttt atccggatcg cgcgcttcag
    33181 cggcagtaat cccaatcagc tccagaccgc cgaaggagaa cataattacc gccagcgaca
    33241 aaatcagccc attccagccg gtggcgaaga aaccaccgta gcgccagagg ttgtcgatac
    33301 tggctttctc gccgccgtga ccagaaaaca gcagccacag gccaaagccg atcataccga
    33361 tgattgccag cactttaatc aacgcaaacc agaactcggt ttcgccatat aagcgcacgt
    33421 tcaccaggtt aacggcgttg atgataataa agaaggcggc agcccaaatc cacgttggaa
    33481 catccgggaa ccagtactgc atatagatgc ccgcagcggt cagctctgcc attcccacca
    33541 gcacgaacat tacccagtag ttccagccag agaggaagcc cgcaaacggt ccccagtatt
    33601 tataggcaaa gtgggcaaat gaaccggata ccggctcttc gaccaccatc tcgccaagct
    33661 ggcgcataat caggaaagcg atgatcccgg cgacgccgta acccagcaat acagccggac
    33721 ccgccatctg aatcgccgga ccaatgccaa gaaacagacc agtaccaatt gcgccaccca
    33781 gcgcaatcag ttgaatatga cggttatgta atccgcgatg aagcgtcggc tcttgattcg
    33841 acgcagtatc ttccgatacg gttgacgcgt ttttcacgcc tttcccctgt gtgtcttttt
    33901 tgttgagggg caccttttaa catttagtgc ccatcgtcgc aagacacaat ccacacggtt
    33961 aaacggggta tcctgctttt atttccgtac ccgatgtcgg tgcagccaca tcagcttata
    34021 cgccgccggg ataataaaca gcgacagcaa aggtgcggtg atcatgccgc caatcatcgg
    34081 tgcggcaatc cggctcatca cctctgaacc agcccccgtt ccccacagaa tcggcagcag
    34141 accggcgata atcaccgcca ccgtcatcgc tttcgggcgc acgcgcagta ccgcgccgtg
    34201 atacaacgcc tcatccagct tctgctcact gaatgtttgc ggattattca acgacggctc
    34261 ggcctctatg gcgtgacgta aatacatcag catcaccacg ccaaattcgg cggcgacccc
    34321 ggcaagggca ataaaaccgg tgcccgttgc cacagaaaga tgaaagccca tccaccacag
    34381 caaccagatg ccgcccacca gcgcaaacgg tacgctgctg ataatcagca acgcttcgcc
    34441 cacccgacgg aatgccagat acaacagcac gaagatgatc atcagtgtca tcggcaccat
    34501 gagtttcagc ttatggttgg cgcgctccag tagctcgaac tgcccggaga atgccacgct
    34561 ggtgcccggt tttaactgca ctttttcagc tatcgctttt tgcaaatcgt gaaccaccga
    34621 caccatgtca cgatcgcggg catcaatata aatccagctc gtcgggcgcg cattctcggt
    34681 tttcagcatc gacggtccgg tagagacttt aacgtcggcc acgtctgcca gggtgatttg
    34741 ctgcttcatc ggcgtcagga tcggtagctg gcgcagtgcc tgcgggctat cgcgccagct
    34801 ttgcggataa cgcagattaa tgggataacg ggcaatccct tccaccgttt cgccaaccat
    34861 cgccccgccc accgcagaag tcacaaacaa ctgcacatcc gccaccgtca taccgtaacg
    34921 cgcggctttt tcacggttaa tctcaacgtt gatatagcgc ccaccttcca gacgctcggc
    34981 tagagcagaa gccacgcttg gcaccgttcg cgctacttct tcaatttgct cagccatcgc
    35041 gtcgatatcc gccagcacag tgccggaaac tttaatgccg atggggcttt taatgccggt
    35101 tgagagcata tcgatacggt tacgaattgg cggcacccac agattcgcca gccccggcag
    35161 ccgcacggtg ttatccagtt cctcaatgat tttgtccatc gtcatgccag gccgccactg
    35221 atcctgcggc ttaagctgga tggtcgtttc aaccatctcg agcggagcag aatcggtggc
    35281 ggtttccgct ttcccggttt tgccaaatac ccgcgccact tcaggtacgc tcataattag
    35341 cttgtcggtt ttttgcagca tactcgccgc ctctgctgcg gaaatccccg gcagcgtcga
    35401 tggcatatac aacaagtcgc cttcattgat ctgcggtaaa aattccccgc caactttatt
    35461 gagcggccag agaaccgtca gcaccgaaag cgctgccacc aacagcgtgg ttttcggcca
    35521 gtgcagtact ttcagcaata gaggatgata aacacgaatc aaaaagcgat tgagcgggtt
    35581 actgctttcc ggcggaattt tgccacggat ccagtacccc atcaggatag ggatcactac
    35641 gatcgccagc agcgccgcgc ccgccatcgc ataggtttta gtgaacgcca acgggccaaa
    35701 cagacgccct tcctgccctt ccagggtgaa gatcgggata aacgacaacg tgataatcag
    35761 cagactgata aatagcgccg gacccacttc aacagatgca tcggtgatca cctgccagcg
    35821 cgttttatta tccagcgtgg cgtcaggatg ctggtgctgc cactcttcca gccgtttatg
    35881 tgcattttcg atcatgacga tagcggcatc gaccatcgcc ccgacggcaa tcgcaatgcc
    35941 gcccaacgac ataatattgg cattcagtcc ctggaagtgc atgacaataa aagcaataca
    36001 caaccccagc ggcaacgaaa taatcgccac cagcgccgag cgcacatgcc agagaaacag
    36061 cgcacagact accgccacca cgataaactc ttccagcaac ttgccgctga ggttgtcgat
    36121 ggcgcggtca atgagctggc tgcgatcgta tgttgtaact atctccacgc cttccggcag
    36181 actacttttc agcgtttcca gtttgtcctt cacggaggcg atcacttctc gggcgttttt
    36241 gccggagcgc aggatcacca ccccgcccgc cacttcgcct tcgccattca gttcggcaat
    36301 gccccggcgc atctccgggc caatctggac cttcgcaaca tcacgcagat aaacgggcac
    36361 gccattttct ctggctttta aaacgatgtg attaaagtcg tcgagcgttt gcagatagcc
    36421 gctggcgcgc accatatatt ccgcttccgc cagttcgatc gacgaaccgc ccgcttcctg
    36481 gtttgaagca tccagcgcgc tttttacttc ggctagactg atgccatatt gcgccaggcg
    36541 ctggggatcg ataaccacct gatactcttt caccacgccg cccaccgacg ccacttccgc
    36601 aacgtcaggg atggttttta gctcatattt gagaaaccag tcctgcaatg agcgtaaatc
    36661 ggccagatcg tgcttaccgc tgcgatccac cagtgcatat tcatagatcc agccaacacc
    36721 cgtggcatct ggtcccagct cggcgctgac tcccgcaggc agcttaccct gtacctggtt
    36781 gaggtactcc agcacccgcg aacgcgccca gtacggatcg gtaccatctt cgaaaatgac
    36841 atacacataa gagtctccaa actgcgagaa accgcgcaca gtcttcgcgc caggcaccga
    36901 caacatggtg gtggttagcg gataagtcac ctgattttca acgatttgcg gtgcctgacc
    36961 gggatagctg gttttaataa tcacctgcac atcggagaga tccggcagcg catccactgg
    37021 cgtgttaatg atggtccagg tgccccagat gctcagaaac aacgcgccca tcagcaccaa
    37081 aaaacggttc gccaccgagc gacgaataat ccattcaatc attggttatt ccctcaatgc
    37141 gcattggtag cactttcaga gcgcatccgc tccagtgcgc cagaaatatt ggcttcggaa
    37201 tcaatcagga acaggccgct ggaaaccacc ttttcacctt ccgccagacc agagtgtaac
    37261 gcggtgacgc cttgcgatgc ctggaaaacg gcaacgcgtt tcggtacaaa gcgtccgtcg
    37321 gcatcaacgg taatcacccg ctgttcgttg ccggtatcaa tcagcgcctg tgacggaatg
    37381 agcagcatcg gttcgctggc ggtgttgagt tgcaaccagg cgttcatgcc cggttttagc
    37441 gcctcgtcgg cgttgtcgac ttccagacgc agctgcagcg tgcgggtcgc ggcatccacg
    37501 ccaggtagca gcgtccattt gcggatggtg agtgttttat ccggtcgcgc cggaacggtg
    37561 agcgtaaact gcgaggcatc tttcaccagc caggcgatag actccgggat cgcagcagtg
    37621 acccacaccg ggtccatacc ctgaattttc gctaccacgt tatctttggc gatattcatt
    37681 cccgcgcgca gatcaaacgc ggtgatcacg ccatcaatgg gtgctttgag cgtaaagcga
    37741 gtctggattt tttgcgtggc gatcaggcgg cgaatatccg cctccggcat tcccgccagt
    37801 cgcagccgct caagaatgcc ttcagtctgg gtcgccgtac cgccggtttc gcgcagcagt
    37861 aaatactcac tctgcgcttc cacccagtca ggaatggtca ggtcgagaag cggtgtgccc
    37921 ttttgtactt tatcgcccac ggtaagcgga tacaccttgt cgataaaccc ggcagcgcga
    37981 gcctgcacaa tggcatactg atactcgttg taactgacat tcgccgggaa actctgggca
    38041 aaagtcagcg gtccgcgcgt gacggtagcc gttttcaccc ccagattctg cgtctgagtc
    38101 gggtcaatgc gcacaccaga cgcagaactc tcttcatcgg catatttcgg caccagatcc
    38161 atatccataa acggcgattt acctggttta tcgaaccgcg tattggggta catcgggtcg
    38221 taccagaata agattttacg ttctgcggtc gacgtttttt ctgcgggtgg ttcagccttt
    38281 gcaaaccagg taaaacctgc cgcagaaata ataccgcccg cgatcatgct gccgataata
    38341 agcgcgattt ttttcatctc attaaacctg ggttactggc tgactttaat atcctgtaat
    38401 aaagaaaggt tgccctgctg gacaaaatta aacgccactt tgtcgccggt tttaatttca
    38461 ctcattttcg tctgcggggt gatggtaaag cgcatagtca tctccggcca gttcacggca
    38521 gcaatcggat cgtgatggat ggtgattttt ttgctttcca gatcaatacc ctttaccacg
    38581 ccagtggcgc taataacctg cggttgtact tcgctcatgg tttcatgatg atgttcgtta
    38641 gcctgggcat taaagccaat aacggtaaac agactgaaca ttgcgacttg cagtgctttt
    38701 ttcatttatt ctctcctgga gttaaaaatt attgctgcca accgccccct aaggcggtat
    38761 acaaagaaat ttcgttaacc tgacgggcat aattcaggtc gagtaaagtt tgtcgggttg
    38821 caaataaaga acgctcggca tccagcactt ccagataact taccgcgccg tgctgatata
    38881 atgcccgcgc ccgttgcaaa gtaatttgca gcgacgccag ataacgctgc tgggcgctga
    38941 tttgatcgtt caggctttga cgtaatgcca gcgcatctgc cacttcttta aaggcgttct
    39001 ggattttctg ttcataattc actaccgact gctgctggcg aatttcggcg atatccagat
    39061 tggcctggtt gcgtccggca ttaaaaatgg ggatctcaat tttaggaata aaattccaca
    39121 tcccgctgct agcgttaaat aatgaagaca gatcgctgct ggcggttgat attccgctgg
    39181 tcaggctgat agacgggaaa aatgccgcac gcgcagcacc aatattagca ttagccgcca
    39241 ttaacgcgtg ttcagcttcc ataatatccg ggcgctgcaa taagatttgc gacgacaagc
    39301 ccgccggtaa tttaacgctt tgcaggctgc cgctgtttac tgtctgcgct tgcggcagtt
    39361 tgccgtagct tcccaataac agttgcaatg cattattcgc ctgcgccagt tccccctgac
    39421 gtttagcgat gtcactgcgg gtactttcta tcaccccgcg agcctgttcc agcgccagaa
    39481 cattgctgct accggtcaac agttgttttt cgacaaacgc atatgactgc tgataattac
    39541 gcagcgtttc ttcggctatt tgcaattgcg catacgccag ttgctgattg aaatagcttt
    39601 gcgcgacatt agaaaccagc agaatatgca ccgcgcgctg agcttcctca gtggctaaat
    39661 aattttgtcg ctcggcttcg ctcatgttct ttaagcgacc gaaaaaatcg aggtcaaagc
    39721 tggcgttaag gccggtcgaa aactcccgtg tcgtggcggt attcccttta agattaccgc
    39781 tccagctgcc gctgccctcg ccattgagct gtgggtagcg gtcggcatcg gtcagacgat
    39841 attgcgcccg tgcctcctgc actttcagcg tcgccatgcg caaatcccgg ttattcacca
    39901 gcgcctcgct aatcagcgtc ttcacctgat tatcaacaaa aaaggtgcgc cagcccacgt
    39961 tctggtagtt atctgctgcg ttaaccaagc cgttctggct gagtgagaac tgctgcggca
    40021 cgggcattgc cgtacgctga taatccggtg ccagtgaaca accggttagc gcaagggcca
    40081 cacaaaatgg cagaagttta caaggagaca taggctcata atttctggtg attttatacc
    40141 gccaacttta ctcgccaggc tctgattttc cggtgacagg aaaatgacaa aattgtcatt
    40201 ttgccaataa gcgattgcca tctgatcccg ctactctaga attgcccggg caacatgcgg
    40261 aggaaaaatg aaactgttga ttgtcgaaga tgaaaagaaa accggagaat acttgaccaa
    40321 agggttaacc gaagccggtt ttgtggtcga tttggccgac aacgggctga atggctacca
    40381 tctggcgatg accggtgatt atgacctgat aatcctcgat attatgctgc cggacgtgaa
    40441 cggctgggat atcgtgcgca tgttgcgctc cgccaataaa gggatgccga ttctgttgct
    40501 taccgcgctt ggcaccattg aacatcgcgt caaggggctg gagttggggg ccgatgacta
    40561 tctggtgaag ccgttcgctt ttgctgaact gctggcgcgg gtgcgcactc tcctgcggcg
    40621 cggggcggcg gtgattatcg aaagtcagtt tcaggttgcc gatctgatgg tcgatctcgt
    40681 cagccgcaaa gtcacccgca gcggcacgcg catcactctg accagtaaag agtttactct
    40741 gctggagttt tttctccgcc atcagggcga agtgctgccc cgctcgctta tcgcctcgca
    40801 ggtgtgggac atgaatttcg acagcgacac taacgccatt gatgtggcgg tgaagcggct
    40861 acgcggcaaa atcgacaacg actttgagcc gaagctgatt cagaccgtgc gcggcgtggg
    40921 ctatatgctt gaggtgccgg atggtcagta agccatttca gcgcccgttt tcgctggcaa
    40981 cccgtctgac cttttttatc agcctggcca ccatcgcggc gtttttcgcc tttgcatgga
    41041 tcatgatcca ctcagtgaaa gtgcattttg ccgagcagga tattaatgat ttaaaagaaa
    41101 ttagcgccac ccttgaacgg gtacttaatc atcctgacga gacgcaagcc cgacgcttaa
    41161 tgacgctgga agatatcgtc agtggttatt ccaacgtgtt gatttccctg gcagatagcc
    41221 acggcaaaac ggtgtatcac tcccccggtg cgccggatat ccgcgagttt acgcgtgacg
    41281 ccatacccga taaagacgct cagggtggcg aggtgtatct cctttccggc ccgacgatta
    41341 tgatgccagg ccacggtcac gggcatatgg aacacagcaa ctggcggatg attaacttgc
    41401 cggttggccc gttggtggac ggcaaaccga tttatacgct ctacatcgcg ctttcgatcg
    41461 attttcatct tcattacata aatgatttga tgaataaact tattatgacc gcatctgtca
    41521 tcagcatcct gatcgtcttt atcgtgctat tggcggtaca taaaggtcac gcgccgatcc
    41581 gcagcgtcag ccgtcaaatc cagaatatca cctcgaaaga tctcgacgtt cgccttgacc
    41641 cgcagaccga gcccattgag ctggaacagc tggtactgtc gttcaaccat atgatcgagc
    41701 gtattgagga tgtctttacc cgccagtcca atttctcagc ggatatcgct cacgaaattc
    41761 gcacgccgat taccaatctc attacgcaaa cggaaatcgc cctcagccag tctcgcagcc
    41821 agaaggagct ggaagatgtg ctctactcta atctcgaaga gctgacgcga atggcgaaaa
    41881 tggtcagcga tatgctgttt ctcgctcagg ccgataacaa ccagctaatc cccgaaaaga
    41941 aaatgctcaa cctggcggat gaagtcggca aagtgttcga ttttttcgag gcgttagcgg
    42001 aagatcgcgg cgtggagttg cgatttgttg gcgacaaatg tcaggttgcg ggcgatccgc
    42061 tgatgctgcg tcgggcgtta agcaacctgc tttctaatgc cctgcgttat acgccaacca
    42121 gagagacaat tgtagtgcgc tgtcagacgg tcgatcacca ggtgcaagtt accgtcgaaa
    42181 accccggtac gcccattgcg cccgagcact taccgcgatt gtttgaccgt ttctaccgcg
    42241 ttgacccttc ccgccagcga aaaggcgaag gtagcggcat tgggctggcg atagtgaaat
    42301 cgattgttgt cgcgcataaa ggcacggttg cagtaacgtc agatgcgcgg gggacaaggt
    42361 ttgtgattat gctgccggag agagagtgat tattaagttc aaattcaggg cgtggtgcat
    42421 cacctcctgg tattgttgat caacaaatta gagaaaaact tattaaacat atgaaatatc
    42481 gaaggctttt atttttcaaa gtttcacgag atgtttgtat atgaaactta gttaaatctg
    42541 tgtttcttcc aattgtagat cacaatgccc tttctaaatt ttcatcgtta taaacaagcc
    42601 atctatcata t
//
LOCUS       DADMKF010000017        40159 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.017, whole genome shotgun sequence.
ACCESSION   DADMKF010000017 DADMKF010000000
VERSION     DADMKF010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40159
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.017"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            <1..494
                     /gene="gstA"
                     /locus_tag="JNC24_000843"
     CDS             <1..494
                     /gene="gstA"
                     /locus_tag="JNC24_000843"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HAY9157667.1"
                     /translation="NGDDYFAVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQL
                     LAPVNSISRYKTIEWLNYIATELHKGFTPLFRPDTPEEYKPTVRAQLDKKLQYVNEAL
                     KDEHWICGQRFTIADAYLFTVLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAE
                     GLK"
     gene            complement(538..1398)
                     /gene="pdxY"
                     /locus_tag="JNC24_000844"
     CDS             complement(538..1398)
                     /gene="pdxY"
                     /locus_tag="JNC24_000844"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase PdxY"
                     /protein_id="HAY9157668.1"
                     /translation="MKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNHT
                     QYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKAA
                     NPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNNV
                     EEAVLAARELIAQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGMRQP
                     VGVGDVTSGLLLVKLLQGATLQEVLEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIAK
                     PEHYFSATKL"
     gene            complement(1460..2734)
                     /gene="tyrS"
                     /locus_tag="JNC24_000845"
     CDS             complement(1460..2734)
                     /gene="tyrS"
                     /locus_tag="JNC24_000845"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HAY9157669.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYGVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            complement(2863..3519)
                     /gene="pdxH"
                     /locus_tag="JNC24_000846"
     CDS             complement(2863..3519)
                     /gene="pdxH"
                     /locus_tag="JNC24_000846"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HAY9157670.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHAQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            complement(3578..3907)
                     /gene="mliC"
                     /locus_tag="JNC24_000847"
     CDS             complement(3578..3907)
                     /gene="mliC"
                     /locus_tag="JNC24_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HAY9157671.1"
                     /translation="MSMKKLLLICLPVLLTGCSAFNQLVEHMQTATLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGISASGVRYTDGIYVFWSKGDEATVYKRDRIVLNNC
                     QLQNPQR"
     gene            complement(4005..5114)
                     /gene="anmK"
                     /locus_tag="JNC24_000848"
     CDS             complement(4005..5114)
                     /gene="anmK"
                     /locus_tag="JNC24_000848"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HAY9157672.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAALLPGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANP"
     gene            5388..5855
                     /gene="slyB"
                     /locus_tag="JNC24_000849"
     CDS             5388..5855
                     /gene="slyB"
                     /locus_tag="JNC24_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HAY9157673.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            complement(5902..6336)
                     /gene="slyA"
                     /locus_tag="JNC24_000850"
     CDS             complement(5902..6336)
                     /gene="slyA"
                     /locus_tag="JNC24_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HAY9157674.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            6537..6773
                     /locus_tag="JNC24_000851"
     CDS             6537..6773
                     /locus_tag="JNC24_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HAY9157675.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            6776..7633
                     /locus_tag="JNC24_000852"
     CDS             6776..7633
                     /locus_tag="JNC24_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HAY9157676.1"
                     /translation="MSIKTIKYFSTIIVAVVAVLAGWWLWNYYMQSPWTRDGKIRAEQ
                     VSITPQVSGRIVELNIKDNQLVNAGDLLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFISAEELDTANLNVKAMQASVNAAQATLKQTQWQLAQTEIRAPVSG
                     RVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LQGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQRVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            7654..9645
                     /locus_tag="JNC24_000853"
     CDS             7654..9645
                     /locus_tag="JNC24_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001376876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAY9157677.1"
                     /translation="MRNLPWFRATLAQWRYALRNTIAMCLALTVAYYLNLDEPYWAMT
                     SAAVVSFPTVGGVISKSLGRIAGSLLGAIAALLLAGHTLNEPWFFLLSMSAWLGFCTW
                     ACAHFTNNVAYAFQLAGYTAAIIAFPMVNITEASQLWDIAQARVCEVIVGILCGGMMM
                     MILPSSSDATALLTALKNMHARLLEHASLLWQPETNDAIRAAHEGVIGQILTMNLLRI
                     QAFWSHYRFRQQNARLNALLHQQLRMTSVISSLRRMLLNWPSPPGATREILEQLLTAL
                     ASSQTDAYTVARIIAPLRPTNVADYRHVAFWQRLRYFCRLYLQSSQELHRLQSGVDDH
                     ARLARTSALARHTDNAEAMWSGLRTFCTLMMIGAWSIASQWDAGANALTLAAISCVLY
                     SAVAAPFKSLSLLMRTLVLLSLFSFVVKFGLMVQISDLWQFLLFLFPLLATMQLLKLQ
                     MPKFAALWGQLIVFMGSFIAVTNPPVYDFADFLNENLAKIVGVALAWLAFAILRPGSD
                     ARKSRRHIRALRRDFVDQLSRHPTLSGSEFESLTYHHVSQLSNSQDALARRWLLRWGV
                     VLLNCSHVVWQLRDWESRSDPLSRVRDNCISLLRGVMSERGVQQKSLAATLEELQRIC
                     DSLARHHQPAARELAAIVWRLYCSLSQLEQAPPQGTLAS"
     gene            complement(9646..10167)
                     /gene="sodC"
                     /locus_tag="JNC24_000854"
     CDS             complement(9646..10167)
                     /gene="sodC"
                     /locus_tag="JNC24_000854"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC2"
                     /protein_id="HAY9157678.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGTGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(10248..11144)
                     /locus_tag="JNC24_000855"
     CDS             complement(10248..11144)
                     /locus_tag="JNC24_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HAY9157679.1"
                     /translation="MVQRITIAPQGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RDHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLKSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            complement(11193..11432)
                     /locus_tag="JNC24_000856"
     CDS             complement(11193..11432)
                     /locus_tag="JNC24_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HAY9157680.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            11535..12134
                     /locus_tag="JNC24_000857"
     CDS             11535..12134
                     /locus_tag="JNC24_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAY9157681.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKIAEVPKGS
                     FYHYFRSKEAFGVAMLERHYATYHQRLTELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGHENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRNSEPLENALAHVKTIIATPAV"
     gene            12171..13268
                     /gene="nemA"
                     /locus_tag="JNC24_000858"
     CDS             12171..13268
                     /gene="nemA"
                     /locus_tag="JNC24_000858"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HAY9157682.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMAVQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQRRAELNPQRAESFYGGGAEGYTDYPTL"
     gene            13349..13756
                     /gene="gloA"
                     /locus_tag="JNC24_000859"
     CDS             13349..13756
                     /gene="gloA"
                     /locus_tag="JNC24_000859"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAY9157683.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            13859..14506
                     /gene="rnt"
                     /locus_tag="JNC24_000860"
     CDS             13859..14506
                     /gene="rnt"
                     /locus_tag="JNC24_000860"
                     /EC_number="3.1.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HAY9157684.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            14599..19168
                     /locus_tag="JNC24_000861"
                     /pseudo
     CDS             14599..19168
                     /locus_tag="JNC24_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
     gene            complement(19219..19566)
                     /gene="grxD"
                     /locus_tag="JNC24_000862"
     CDS             complement(19219..19566)
                     /gene="grxD"
                     /locus_tag="JNC24_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HAY9157685.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGKLVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            19900..20715
                     /locus_tag="JNC24_000863"
     CDS             19900..20715
                     /locus_tag="JNC24_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HAY9157686.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKTAKKAASKSTTKSKTASSVKKSSITASKNAKTRSKHAVNKTASA
                     SFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPYRWGG
                     SSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAAPIERSELKNGDLVFFRTQG
                     RGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            20843..21424
                     /gene="sodB"
                     /locus_tag="JNC24_000864"
     CDS             20843..21424
                     /gene="sodB"
                     /locus_tag="JNC24_000864"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HAY9157687.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(21570..22739)
                     /locus_tag="JNC24_000865"
     CDS             complement(21570..22739)
                     /locus_tag="JNC24_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY9157688.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGTGARPEVKKELAVLMRPQVLPALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLATPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMRVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVMGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(22905..22994)
                     /gene="ynhF"
                     /locus_tag="JNC24_000866"
     CDS             complement(22905..22994)
                     /gene="ynhF"
                     /locus_tag="JNC24_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein YnhF"
                     /protein_id="HAY9157689.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            23293..24318
                     /gene="purR"
                     /locus_tag="JNC24_000867"
     CDS             23293..24318
                     /gene="purR"
                     /locus_tag="JNC24_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HAY9157690.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLVGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            complement(24315..25247)
                     /locus_tag="JNC24_000868"
     CDS             complement(24315..25247)
                     /locus_tag="JNC24_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY9157691.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     VVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            25360..26571
                     /locus_tag="JNC24_000869"
     CDS             25360..26571
                     /locus_tag="JNC24_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
                     /protein_id="HAY9157692.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGMTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIIPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEAGCQNH
                     GNAEVAHSESH"
     gene            26862..28010
                     /gene="cfa"
                     /locus_tag="JNC24_000870"
     CDS             26862..28010
                     /gene="cfa"
                     /locus_tag="JNC24_000870"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HAY9157693.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCEGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(28050..28691)
                     /gene="ribE"
                     /locus_tag="JNC24_000871"
     CDS             complement(28050..28691)
                     /gene="ribE"
                     /locus_tag="JNC24_000871"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HAY9157694.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            28906..30279
                     /gene="mdtK"
                     /locus_tag="JNC24_000872"
     CDS             28906..30279
                     /gene="mdtK"
                     /locus_tag="JNC24_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HAY9157695.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRMPSAI
                     ILQRASR"
     gene            complement(30320..31576)
                     /locus_tag="JNC24_000873"
     CDS             complement(30320..31576)
                     /locus_tag="JNC24_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9157696.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPSGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGAELQQSTITVQQGGVLILDGSTVKGDSVTFIVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVNGDIRVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            31884..31960
                     /locus_tag="JNC24_000874"
     tRNA            31884..31960
                     /locus_tag="JNC24_000874"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31918..31920,aa:Val,seq:gac)
     gene            31965..32041
                     /locus_tag="JNC24_000875"
     tRNA            31965..32041
                     /locus_tag="JNC24_000875"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31999..32001,aa:Val,seq:gac)
     gene            32149..32454
                     /locus_tag="JNC24_000876"
     CDS             32149..32454
                     /locus_tag="JNC24_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HAY9157697.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            32580..34184
                     /locus_tag="JNC24_000877"
     CDS             32580..34184
                     /locus_tag="JNC24_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HAY9157698.1"
                     /translation="MKKIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADEAGVGMPY
                     SDEENSKMMLANIASIEIPPINCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARQQKFAVAVYESCQVTDLQITNAGVMLATNQDLPSETFDLAVI
                     ATGHVWPDEEEATRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMAVAIQHGSFI
                     EDDKQHVVFHRDNASEKLNITLMSRTGILPEADFYCPIPYEPLHIVTDQALNAEIQKG
                     EYGLLDRVFRLIVEEIKFADPDWSQRIALESLNVDSFAQAWFAERKQRDPFDWAEKNL
                     QEVERNKREKHTVPWRYVILRLHEAVQEIVPHLNEHDHKRFSKGLARVFIDNYAAIPS
                     ESIRRLLALREAGIIHILALGEDYKMEINESRTVLKTEDNSYSFDVFIDARGQRPLKV
                     KDIPFPGLREQLQKTGDEIPDVGEDYTLQQPEDIRGRVAFGALPWLMHDQPFVQGLTA
                     CAEIGEAMARAVVKPASRARRRLSFD"
     gene            complement(34196..35008)
                     /locus_tag="JNC24_000878"
     CDS             complement(34196..35008)
                     /locus_tag="JNC24_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9157699.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIHHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGEIPFCSQFARSDDHARIGCCED
                     NARIVTAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGPGGSAALAYHDGERVRFAVAIEGENNIRTGVRYRLNEQHQFVEC"
     gene            complement(35012..35797)
                     /locus_tag="JNC24_000879"
     CDS             complement(35012..35797)
                     /locus_tag="JNC24_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HAY9157700.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSATDHGEKVYLYSKAVRLWHWSNALLFVLLLASGLINHFAMVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINAVGDNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMCGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            complement(35794..36462)
                     /locus_tag="JNC24_000880"
     CDS             complement(35794..36462)
                     /locus_tag="JNC24_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HAY9157701.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQENKYYQYQLLGAGKPHLYRRFGQHLIK
                     KENV"
     gene            complement(36526..37164)
                     /locus_tag="JNC24_000881"
     CDS             complement(36526..37164)
                     /locus_tag="JNC24_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HAY9157702.1"
                     /translation="MNHQDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARTVFLSPPYSVAEEELSVLLESIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            complement(37177..39285)
                     /locus_tag="JNC24_000882"
     CDS             complement(37177..39285)
                     /locus_tag="JNC24_000882"
                     /EC_number="1.2.7.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
                     /protein_id="HAY9157703.1"
                     /translation="MANGWTGNILRVNLTTGNITLEDSSKFKSFVGGMGFGYKIMYDE
                     VPPGTKPFDEANKLVFATGPLTGSGAPCSSRVNITSLSTFTKGNLVVDAHMGGFFAAQ
                     MKFAGYDVIIIEGKAKSPVWLKIKDDKVSLEKADFLWGKGTRATTEEICRLTSPETCV
                     AAIGQAGENLVPLSGMLNSRNHSGGAGTGAIMGSKNLKAIAVEGTKGVNIADRQEMKR
                     LNDYMMTELIGANNNHVVPSTPQSWAEYSDPKSRWTARKGLFWGAAEGGPIETGEIPP
                     GNQNTVGFRTYKSVFDLGPAAEKYTVKMSGCHSCPIRCMTQMNIPRVKEFGVPSTGGN
                     TCVANFVHTTIFPNGPKDFEDKDDGRVIGNLVGLNLFDDYGLWCNYGQLHRDFTYCYS
                     KGVFKRVLPAEEYAEIRWDQLEAGDVNFIKIKDFYYRLAHRVGELSHLADGSYAIAER
                     WNLGEEYWGYAKNKLWSPFGYPVHHANEASAQVGSIVNCMFNRDCMTHTHINFIGSGL
                     PLKLQREVAKELFGSEDAYDETKNYTPINDAKIKYAKWSLLRVCLHNAVTLCNWVWPM
                     TVSPLKSRNYRGDLALEAKFFKAITGEEMTQEKLDLAAERIFTLHRAYTVKLMQTKDM
                     RNEHDLICSWVFDKDPQIPVFTEGTDKMDRDDMHASLTMFYKEMGWDPQLGCPTRETL
                     QRLGLEDIAADLAAHNLLPA"
     gene            complement(39306..39932)
                     /locus_tag="JNC24_000883"
     CDS             complement(39306..39932)
                     /locus_tag="JNC24_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HAY9157704.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
BASE COUNT         9642 a        10553 c         9887 g        10077 t
ORIGIN      
        1 aaaacggtga cgattacttt gccgttaacc ctaaggggca ggtgccagca ttgctgctgg
       61 atgacggtac tttgctgacg gaaggcgtag cgattatgca gtatcttgcc gacagcgtcc
      121 ccgaccgcca gttgctggca ccggtaaaca gtatttcccg ctataaaacc atcgaatggc
      181 tgaattacat cgccaccgag ctgcataaag gtttcacacc tctgtttcgc ccagatacac
      241 cggaagagta caaaccgaca gttcgcgcgc agctggataa gaagctgcaa tatgtgaacg
      301 aggcactgaa ggatgagcac tggatctgcg ggcaaagatt tacaattgct gatgcctatc
      361 tgtttacggt tctgcgctgg gcatacgcgg tgaaactgaa tctggaaggg ttagagcaca
      421 ttgcggcatt tatgcaacgt atggctgaac gtccggaagt acaagacgcg ctgtcagcgg
      481 aaggcttaaa gtaatagcca tcggcccaca ggtcgttgtg ggccgaaatg tgatatttca
      541 gagctttgtt gcgctgaagt aatgttctgg tttggcaata cgatcctgag cagccaccac
      601 ttgcagctca tattcctgca ttgctttggt ggtcaccatg atttcgtaga ctgcagcggt
      661 cacatgttcc aggacctcct gcagcgttgc cccctgaagc agtttcacca gcagtaaacc
      721 gctcgtcaca tcaccaacac ctaccggctg gcgcatacca aaatccacca gtggacggct
      781 gatatgccag gcttcatcgg cggtgaccag cagcatttca aaacggtcac ggctgtagcc
      841 agctcgcgcc aggtgtttaa ccaacacaat ttgtggccct tgcgcaatga gttcgcgcgc
      901 tgccagaacc gcttcttcga cgttatttac cgcatgctca cagagtattt ccagctcaac
      961 cagattcggc gcaatgatat cgctggcagg caaaccgtgc cgcacatgaa actctgcgac
     1021 acccggtgca acgatacagc ctttttccgg atgccccatt accggatcgc aaaaatattt
     1081 cgcctgcgga ttcgctgctt tcacctgacg gacgataccg aggatatgtt caccctgctc
     1141 cgccgatccc agatagccac ttaacacggc atcacaggtg tgtaatttat caatagcggc
     1201 aatgccttgc acaatttcgg ttaaatggct gggcggcatc acgcagccag tccatttgcc
     1261 gtattgggtg tgattagaaa attgaacggt gttcagcggc cagacgttcg cgcccaggcg
     1321 gcgcatcgga aactctgccg cactgttacc cgcatgacca taaacaacgt gagactggat
     1381 agcgagaata tttttcatca tgttccctgt ataaaaacca gggggagtga tttctcactc
     1441 cccctttcca cttaatgcat tatttccagc aaatcagaca gtaattcttt ttgccgcgac
     1501 gcagtaaggt aaaacgacca aacagacgat cttcttcttt aaagaagtat tcaggatcgg
     1561 actgtttttc accgttaatg gtgatggcat tggaggcgat agttttacgc gcctgaccac
     1621 gggaaggttg cagttcagaa tcgaccagcg cctgcatcag gtctgcgccc ttttccatct
     1681 caaccatcgg tacgccgtcc tgcgccagct gttcgaagtc cgcttcactc agcgcactca
     1741 aggaaccgct gaacaggcat tcggtaatac gttttgccgc ctgcaaacct tcttcaccgt
     1801 gaaccagacg agtcacctgc tccgccagta catactgggc gcgcggtgct ttaccgctgt
     1861 ttttatcttc ttcttccagg gcgttgatct cttcaatgct cataaaggtg aagaacttca
     1921 ggaagcggta aacgtcggca tccgcagtgt tgatccagaa ctggtagaat ttgtacgggc
     1981 tggttttctt cggatccaac cagactgcgc cgccttcagt tttaccaaat ttggtgccgt
     2041 ctgctttggt gatcagcgga acggtcaggc caaacacctg attctgatgc agacgacggg
     2101 tcaggtcgat accagaagtg atgttacccc actggtcaga accaccaatt tgcagcacca
     2161 caccgtactg tttgttcaga caggcgaagt cataaccctg caacaggttg taggaaaact
     2221 cagtgaacga aatcccctga tcttcacggt tgagacgctg cttaaccgct tctttgttga
     2281 tcatctggtt aacggagaag tgtttgccaa tatcgcgcag gaaggtcagc acattcatat
     2341 tgccgaacca gtcatagttg ttcgccgcga tagcagagtt ttctccacag tcgaaatcaa
     2401 ggaacggggc aacctgcttg cggattttgt ccacccactc ctgaacagtt tcttcggtgt
     2461 tcagcttacg ctcggcagct ttgaagctcg ggtcgccaat cagacccgtc gcgccgccta
     2521 ccagcgcaac cggcttgtgg cccgcctgct ggaagcgttt caggcataac aatggaacaa
     2581 gatgccccaa atgcaagctg tcagcggtag gatcgaagcc gcaatagagc gcgatcgggc
     2641 cttgcgccag tcgctctgct aacgcttcct cgtccgtcac ctgggctacc agcccccgct
     2701 cttgcaattg tttaatcaag ttactgcttg ccatcaaaat ctccatgtat ataacgactg
     2761 cacctttgcc ggtacacgac ttttcgccag atgcgaaaga gacatagaat aaagtgccag
     2821 aatcaggagt accagcgatt aaagcaagat ttttgcatct tttcagggtg caagacgatc
     2881 aatcttccac gcatcatttt cacgctggta caaaaagcgg tcatgcaggc gatgctcacc
     2941 gccctgccag aactcaatct gttcaaggct gacgcgaaaa ccgccccaaa agctcggcaa
     3001 tggcacttcg ccctgttgaa acttctgctt cagctccagg aatttacttt caaggatacc
     3061 gcgggcagaa atgcgactgg actgcttcga aacccatgca ccaatctggc tatcacgcgg
     3121 gcggctatga aaatatttca tcacttcgag agtcgaaagt cgttccgctt taccgatcac
     3181 catcacctgg cgctcaaggg tatgccacgg gaacagcagg ctaacgcgcg gattattttc
     3241 aatttgatgt gctttacggc tgccgaggtt ggtgtaaaac accatgcctt tttcgtcgta
     3301 atgtttgagt aaaacgatgc gttgataagg ctgagcatgt tcatccacgg tagcgaccac
     3361 catcgcggta gggtctgcca gtttggcttc acacgcctga gagagccaac gttcaaaaag
     3421 ggttaatgga tcggcgggaa gatcgcgacg gcgtaacccg cctttggtgt attcacggcg
     3481 cagatgcgcg atttgctgca attcgtcgtt atcagacatg gttttcttta cggattgtca
     3541 gtgggtgacg ctattgtgcg ccgcccctgg aaaaatctca acgctgtgga ttttgtaact
     3601 gacagttatt caatacgatg cggtcgcgtt tatagacagt cgcttcatcg cctttcgacc
     3661 agaaaacata gattccgtca gtgtaacgta caccagacgc tgaaatgccc tgtttgagat
     3721 gcagtagttg attatcgtaa acaaaactga cttcctggcg cggattattc agtttgaccg
     3781 tcaacggttt ttcatcacac tggtattcca gcgtagcggt ttgcatgtgc tcaaccagtt
     3841 gattgaacgc gctacagccg gtgagaagca ccggcaaaca gatcagtaac agttttttca
     3901 tagacatttc ccgaagactt tcctggtctg gagggcaata cgccctccct aacgtttcaa
     3961 gtgtaacggc agacgcggta agaaaaattc agttaactct gatatcacgg gttagcaggg
     4021 aaaatagccc ccagtaccgt ctcctggctt gcgccagtga cggaaggcag atttcctggt
     4081 aatcccgcca gcgtccgcca ggcaagccag gcgaatgcca atgcttccat gtcatcgcca
     4141 ctaatgccaa cggcatcggt ggtggtgact tctgtgcctg gcagtaatgc cgccagacgc
     4201 gccatgagta gcgggttacg actacctcca ccacatacca tcaatcgttc gcagccaccg
     4261 ctcaacaaaa cttgttcaga aatggtcacg gcggtgagtt ctgccagtgt ggcctgaaca
     4321 tcacggggat caacacccgg aaaatggcgt aaatggcgct ccagccagcc gtagttaaag
     4381 tattcgcgtc cggtgctttt cggtgcaggt tgcgagaaat atgggtcgct gagcatattt
     4441 tgcagcagtg ggagaataac tttacccgcc cgtgcccact cggcatcttt atcgtaaggt
     4501 ttaccggcct gacgccagat ccaggcatcc atcagcatgt taccaggacc ggtatcgtag
     4561 ccgccaaccg gctgcccagg aatgagcagt gacagattgg cgatgccgcc aatattgagc
     4621 accattcgtc gctcggttgg gtgagccagc aaggcatgat ggaacgcagg taccagcggt
     4681 gcgccttgtc ctcccaaggc aatatcgcga cggcgaaaat caccgacaac cgtaattccg
     4741 gtgcgtgcca caatttgatt gttatcgcca atctgtaaag tgtgtggtgc cacgcccgtc
     4801 ggttcatgcc agacggtttg accgtgacag ccgatcgcaa ctatatctct cgcctgcagg
     4861 ttttgttcct taagcaaggc attgaccgca tcagcaaaaa gtcgcccgag ttgagtatca
     4921 agctgtccaa actgcgaaag tgtaagctgc tggccctggc aaatatccag tacagcctgt
     4981 ttcagagaca ccgggatcgg ccagctcaag cttgccagct gtgcgacccg gtgttcatct
     5041 attgtcgcca acacaacatc aacaccatca aggctggtgc ctgacataac gccaataaag
     5101 cggcccgatt tcatagttca tcctttttca atctgacgtt tgcgcaccac tcaaacataa
     5161 acttttcgtg gataccatgc ggaatgaccg atttttaccg ttggtagtaa aacattatct
     5221 tcaaatcaat aatcatcatg aatgttttgt ttataattgg ttgatcctac tttcattatg
     5281 atttgctcat atttggtaga acatgtaacc atggattcac atatgccata tactttgacc
     5341 atgagggatg cttgcgtggc gtttcatggt gaacaggaga tttttcaatg attaaacgcg
     5401 tattggttgt ttcaatggta ggtctgtctc ttgtcggttg tgttaataac gacaccctgt
     5461 caggggatgt ttataccgct tctgaagcga aacaagtaca gaatgtcagc tatggcacca
     5521 tcgttaacgt acgtccggta cagattcagg gcggtgatga ttccaacgtt atcggtgcaa
     5581 ttggcggtgc tgttcttggt ggtttcctgg ggaatactgt tggtggcgga accgggcgtt
     5641 ctctggctac tgcagcaggc gctgttgcag gtggcgtagc tggtcagggc gtacagagtg
     5701 caatgaacaa aacgcagggt gtcgagctgg aaattcgtaa agacgatggt aataccatca
     5761 tggtggtaca gaaacaaggc aacactcgtt tctctccggg ccaacgtgtc gtactggcca
     5821 gcaatggcag tcaggtgacc gtttctccgc gctaaataag tttgcgtgtg gtcaggttac
     5881 tgaccacacg cccccttcat ttcacccttt ggcctgtaac tcaatgatat tatgctcaag
     5941 ttttgcgatg agcgtaatca gttgctccag ttcctctgcg gagatgccat gtaatatttc
     6001 cgcgcgggtt ttgttaataa cagcttccat ttcgctgatc agtggctctg ccttttccgt
     6061 cagtttaata cgtttagccc gacgatcgct ggcacaagtt tgacgcgaaa ttaacccttt
     6121 ttcttccagt tggtccagag tacggaccag tgatggctgc tcgatgccaa tcgcttttgc
     6181 cagttgaatt tgcgactggt ctggaggtaa ctgatggata ttgtgtaacg taacccaatg
     6241 ggtttgtgtt aactccagcg gtttcaggcg atggtctatc agagcacgcc atatgcgcac
     6301 caaccgtgcc agatcagaac ctagtggcga ttccaatttc atctccttat aattagcttg
     6361 ctaagatatt atgcggcttt tagaatagtg tgcagcaatt gtattgctaa aacaaatgta
     6421 ttgctgcatt tggttaccgt cagacatatt tttcagaaat tgcgcgtaaa tttttcgcac
     6481 ttaaagaata tttattaatc taacgcaata tattcggtcg taaaggaatc tactttgtga
     6541 agtttatgct caatgcaaca ggattgccct tacaagatct ggtgttcggt gcgtccgtct
     6601 attttcctcc gtttttcaaa gcattcgcgt ttggattcgt catctggctt gtcgtacacc
     6661 gcctgcttcg tggctggatc tacgccggtg acatctggca tcccttgtta atggatttat
     6721 cgctgtttgc gatttgcgtt tgccttgctc tggcaatact gattgcgtgg taactatgtc
     6781 aattaaaaca attaagtatt tctcaacaat cattgtagcg gtagttgcgg ttcttgccgg
     6841 atggtggctg tggaattatt acatgcaatc tccgtggaca cgcgacggga aaatacgcgc
     6901 agaacaggtt tctatcaccc cacaggtgtc gggacgtatt gttgagctga atataaaaga
     6961 caaccagctg gttaacgcag gggatcttct gctcacgatc gataaaacgc cctttcagat
     7021 cgccgagctg aacgcacagg ctcagctggc gaaagcgcaa tctgacctcg caaaagccaa
     7081 taacgaagcg aatcgtcgtc gtcatctctc acaaaatttt atttctgccg aagaactcga
     7141 taccgctaac ctcaatgtta aagcgatgca ggccagtgtt aatgccgcac aggccacgct
     7201 gaaacagaca caatggcaac tggcgcaaac ggaaattcgc gccccggtga gtggacgggt
     7261 gactaacctc actacccgca tcggcgatta cgctgacacc gggaaaccgc tttttgccct
     7321 tgtcgacagc cactcgtttt acgtcattgg ttattttgaa gaaaccaagt tgcgccatat
     7381 ccgcgaaggt gcaccagcac aaattaccct ctatagtgat aacaaaacgt tacagggtca
     7441 cgtttccagt atcggtcggg cgatttatga tcagagcgtt gaaagtgact caagccttat
     7501 tccggatgtg aaacctaacg tcccctgggt tcgtctcgcc caacgcgttc ccgtccgctt
     7561 tgcgctggat aaagttccag gcgatgtcac gctggtgtcg ggcaccacct gtagcattgc
     7621 cgtaggtcaa taatgaacgc atcgtaatgg tccttgcgca atttgccctg gttcagggcc
     7681 acgctggcgc aatggcgtta tgcgttacgc aataccattg ccatgtgtct ggcgctgacg
     7741 gttgcctatt atttgaatct ggatgaaccc tattgggcga tgacctcggc tgcagtggtt
     7801 agctttccca ccgttggcgg tgtcatcagc aaaagcctcg gacgcatcgc tggcagttta
     7861 ctgggagcca ttgcggcact gcttcttgcc ggacatacgc tcaatgagcc gtggtttttt
     7921 ctattgagca tgtcggcgtg gcttggcttt tgtacctggg cctgtgcgca cttcacgaat
     7981 aacgtcgcgt atgcatttca actggcgggc tacacggctg ccatcatcgc ctttccgatg
     8041 gttaatatta ctgaggccag ccagctgtgg gatatcgctc aggcgcgcgt ttgcgaggtg
     8101 atagtcggta ttttgtgcgg cggcatgatg atgatgatcc tgcctagcag ttccgatgct
     8161 actgcccttt taactgcatt gaaaaacatg cacgcccgat tactggaaca tgccagttta
     8221 ctctggcagc ctgaaacaaa cgatgccatt cgtgcagcac atgagggggt gattgggcag
     8281 atactgacca tgaatttgct gcgtatccag gctttctgga gccactatcg ttttcgccag
     8341 caaaacgcgc gccttaatgc gctactccac cagcaattac gtatgaccag tgtcatctcc
     8401 agcctgcgac gtatgttgct caactggccc tcaccgccag gtgccacgcg agaaattctc
     8461 gaacaattgc tgactgcgct cgccagttcg caaacagatg cttacaccgt cgcacgtatt
     8521 atcgccccgc tacgcccgac caacgtcgcc gactaccggc acgtcgcctt ctggcagcga
     8581 ctacgttatt tttgccgcct ttatctgcaa agtagtcagg aattacatcg tctgcaaagc
     8641 ggtgtagatg atcatgccag actcgcacgg acatccgctc tggctcgtca taccgacaac
     8701 gccgaagcta tgtggagcgg gctgcgtaca ttttgtacgt tgatgatgat tggcgcatgg
     8761 agtattgcat cgcaatggga tgccggtgcc aatgcattaa cgctggcagc aattagctgc
     8821 gtactctact ccgccgtcgc agcaccgttt aagtcgttgt cactgctgat gcgcacgctg
     8881 gtgttacttt cgctattcag ctttgtggtc aaatttggcc tgatggtcca gattagcgat
     8941 ctgtggcaat ttttactgtt tctctttcca ctgctggcga caatgcagct tcttaaattg
     9001 cagatgccca aatttgccgc actgtggggg caactgattg tttttatggg ttcgtttatc
     9061 gctgtcacta atcccccggt gtatgatttt gctgattttc ttaacgagaa tctggcaaaa
     9121 atcgttggcg ttgcgctggc ttggctagcg ttcgccattc tgcgtccagg atcggatgct
     9181 cgtaaaagcc gccgccatat tcgcgcgctg cgccgggatt ttgtcgatca gctaagccgc
     9241 catccaacac tgagtggaag cgaatttgaa tcgctcactt atcatcacgt cagtcagttg
     9301 agtaacagcc aggatgcgct ggctcgccgt tggttattac gctggggtgt agtgctgctg
     9361 aactgttctc atgttgtctg gcaattgcgc gactgggaat cgcgttccga tccgttatcg
     9421 cgagtacggg ataactgtat ttcactgttg cggggagtga tgagtgagcg tggcgttcag
     9481 caaaaatcac tggcggccac acttgaagaa ttacagcgga tttgcgacag ccttgcccgt
     9541 catcatcaac ctgccgcccg tgagctggcg gcaattgtct ggcggctgta ctgctcgctt
     9601 tcgcaacttg agcaagcacc accgcaaggt acgctggcct cgtaattact taattacacc
     9661 acaggcatag cgttcaccgc caccgcccag cggtttaggt tgatcggaca tattatcgcc
     9721 gccaacgtgg accatcagcg ccttgtcttt gatttcatcc agtgatttca gacgaggcgc
     9781 gatgacggca tcggtagctt tgccgtcatt attgacgacc agtgcaggca gatcgcctaa
     9841 atgcccggta ccttctggcc cttcatgttt accggtattt tgtggatcaa gatgcccgcc
     9901 tgcggattcc gcggcgctgg ctttgccatc tttggtggct ggctggcagc ttcctttggc
     9961 atgaatatgg aagccatgtt caccgggggg taatgctttc agatcgggtg aaaactccag
    10021 acctttatcg gtttcagtaa tggtgacgct accaattgac tgcccaaccc cttgcgacgt
    10081 gacgaggttc atctcgactt tttcactggc agcttgtgcg ccggtcgcaa caaccagcgc
    10141 cagaatggcc agactaaaac gtttcatagg acctccgttc attgtgcatt cctgaataag
    10201 tgtaaaccag tgacacattt ttgaacggcg gctattccta aaagtgctta cggtacgtcg
    10261 taccccagtg ccgctttacg gatacgaaac cattgttgac gggtcatttt cagtgtttct
    10321 gcttcgacag ctgcccgtac gcgctcaatt ttacctgagc cgataattgg cagcggctgc
    10381 gacggtaaac gtaataccca ggcgtaaacc acctgttcaa tcgagcccgc gtttaactcc
    10441 tccgccacca cagccagttc atcacgcagc ggctggaaat aatcatcatt aaacagacga
    10501 ccaccaccaa ggcaggacca cgccatcgga cgaacacgca gttgttgtag ttggtcgagc
    10561 gtgccatcca gcagtaacgg ctgatgcacc ggggatattt ccacctgatt agtggcaagg
    10621 gtaaacggca gacgtgattt caacagggca aattgcgcag gcgtaaagtt cgatacgcca
    10681 aaatgacgca ctttgccgct ctgatgcaga tgtttgaacg cgtccgccac ttcatcggca
    10741 tccattaacg ggtctggtcg gtggattaac agcagatcca gatgatcggt cgcgagatta
    10801 attagcgact gttcggcgct cttaatgatg tgatcgcggt cagtgatgta atgaccaatg
    10861 acgttttctt cacgcgcggt cgtcgcgata ccgcatttac tgacgatttc catccgttca
    10921 cgcaggtgag gtgccagttt cagtgcctcg ccaaacgccg cttcgcactg atagccacca
    10981 taaatatcag catggtccac ggtggtcacg ccgagatcca gatgctcttc aataaaactg
    11041 accagctggc gggcggacat attccagtcc atcaatcgcc agtagcccat cacaaaacgg
    11101 gaaaactccg ggccttgcgg cgcaatagta atacgctgaa ccataatcgc ttcctcttat
    11161 cagatatgag aggagtatac gcaagattag gttcaaaaga gtgatggttg ctccggttcg
    11221 tctgatgacg ctggcttatt tgcgcgtaat ttgcgcatta atcgctgccg acaaaggcgc
    11281 agcacctctt gtttttcgcc atcgctcatt ttattccagt taaaacgctc atcccgacta
    11341 cgaaaacagc cgcgacaaaa cccgcgttcg tcagactggc aaattccccg gcacgggctc
    11401 tggacgggaa agaactctaa ttgctccgcc acttcgccct cctcaaataa gattattacc
    11461 attattgaag ctgttaatgt ccaaagtagc aactttgctt gcactagacc gactggtcta
    11521 ctacactcca acgcatgaac aaacacaccg aacatgatac tcgcgaacat ctcctggcga
    11581 cgggcgagca actttgcctg caacgtgggt tcaccgggat ggggctaagc gaattactaa
    11641 aaatcgctga agtgccgaaa gggtccttct atcactactt tcgctctaaa gaagcgtttg
    11701 gcgttgctat gcttgagcgc cattacgcca catatcacca gcgactgact gagttgctgc
    11761 aatccggcga aggtaactac cgcgaccgca tactggctta ttaccagcaa acactgaacc
    11821 agttttgcca acatggaacc atcagtggtt gcctgacagt aaaactctct gccgaagtgt
    11881 gcgatctgtc agaagatatg cgtagcgcga tggataaagg cgctcgcggc gtgattgccc
    11941 tgctctctca ggcgctggaa aatggccatg agaaccactg tttaaccttt tgtggcgaac
    12001 cgctgcaaca ggcacaagtg ctttacgcac tatggctggg tgcgaatctg caggccaaaa
    12061 tttcgcgcaa ttccgagcca ctggaaaacg cgctggcaca tgtaaaaacc attattgcga
    12121 cgcctgccgt ttagcaggca ttttttatca ccagacgacc gggagccttt atgtcatctg
    12181 aaaaactgta ttccccactg aaagtgggcg caataactgc ggcaaaccgt atttttatgg
    12241 caccgctgac gcgtctgcgc agtattgaac cgggtgacat ccctaccccg ttgatggcgg
    12301 aatactatcg ccaacgtgcc agtgccggtt tgattattag cgaagccacg caaatttctg
    12361 cccaggcaaa agggtatgct ggtgcacctg gcatccatag tccggaacaa attgccgcat
    12421 ggaaaaaaat taccgctggc gttcatgctg aaaatggtca tatggccgta cagctgtggc
    12481 acactggacg catttctcac gccagcctgc aacctggcgg tcaggcaccg gtagcgcctt
    12541 cagcacttag cgcgggaaca cgtacttctc tgcgcgatga aaatggtcag gcgatccgtg
    12601 ttgaaacatc catgccgcgt gcgcttgaac tggaagagat tccaggtatc gtcaatgatt
    12661 tccgtcaggc cattgctaac gcgcgtgaag ccggttttga tctggtagag ctccactctg
    12721 ctcacggtta tttgctgcat cagttccttt ccccttcttc aaaccatcgt accgatcagt
    12781 acggcggcag cgtggaaaat cgcgcacgtt tggtactgga agtggtcgat gccgggattg
    12841 aagaatgggg tgccgatcgc attggcattc gcgtttcgcc aatcggtact ttccagaaca
    12901 cggataacgg cccgaatgaa gaagccgatg cactgtatct gattgaacaa ctgggtaaac
    12961 gcggcattgc ttatctgcat atgtcagaac cagattgggc gggaggtgaa ccgtatactg
    13021 atgcgttccg cgaaaaagta cgcgcccgtt tccacggtcc gattatcggc gcaggtgcat
    13081 acacggtaga aaaagccgaa acgctgattg gcaaagggtt aattgatgcg gtggcatttg
    13141 gtcgtgactg gattgcgaac ccggatctgg tcgcccgctt gcagcgcaga gctgagctta
    13201 acccacagcg tgccgaaagt ttctacggtg gcggcgcgga aggctatacc gattacccga
    13261 cgttgtaatc caacattgcg agcggcgtaa agccgccgct atactaaaac aacattttga
    13321 atctgttagc cattttgagg ataaaaagat gcgtcttctt cataccatgc tgcgcgttgg
    13381 cgatctgcaa cgctccatcg atttttatac caaagtgctg ggcatgaaac tgctgcgtac
    13441 cagcgaaaac ccggaataca aatactcact ggcgtttgtt ggctacggcc cggaaaccga
    13501 agaagcggtg attgaactga cctacaactg gggcgtggat aaatacgaac tcggcactgc
    13561 ttatggtcac atcgcgctta gcgtagataa cgccgctgaa gcgtgcgaaa aaatccgtca
    13621 aaacgggggt aacgtgaccc gtgaagcggg tccggtaaaa ggcggtacta cggttatcgc
    13681 gtttgtggaa gatccggacg gttacaaaat tgagttaatc gaagagaaag acgccggtcg
    13741 cggtctgggc aactaatctc ctgccgggcg tgaactcatc gcgcccgcat ctttactgca
    13801 tcgacaagta atatttgtca taatgcgcgc tgcaatttat ccgtattaag agaatcagat
    13861 gtccgataac gctcaactta ccggtctgtg cgaccgtttt cgtggttttt atcctgttgt
    13921 gatcgatgtt gaaacagccg gatttaacgc caaaaccgat gcgctgcttg agattgccgc
    13981 catcaccctg aaaatggatg aacaaggctg gctgatgccg gacaccacat tacatttcca
    14041 cgtcgaacca tttgtcggcg caaatttgca gccagaggcc ctcgccttca acggcattga
    14101 cccgaacgat cccgatcgcg gcgcggtcag cgaatacgag gcgctgcacg aaatttttaa
    14161 agttgtacgt aaaggtatta aagcgagcgg ctgtaaccgc gccattatgg tggcgcacaa
    14221 tgccaatttt gatcacagct ttatgatggc cgccgcagaa cgcgcctcac tgaaacgtaa
    14281 cccgttccac cctttcgcca cttttgacac tgctgcactg gccgggctgg cactcggaca
    14341 aaccgtattg tcaaaggctt gccagaccgc tggcatggac ttcgacagca cccaggcgca
    14401 ctccgcgctg tacgacaccg aacgcactgc tgtgctgttt tgtgaaatcg tcaaccgctg
    14461 gaaacgtctg ggaggctggc cgctacctgc cgccgaagag gtgtaatcga gtcgatgcct
    14521 gatgacatgc aatgattcag gcatctatag tgaggctatt ccacgcatca agcatgatat
    14581 tcacggggaa tagcgttaat ggcagataat ccagaccctt catcgctcct gccggacgtg
    14641 ttttcactgg cgacccgcga ctggtttctt cgcgccttta aacagccgac cgctgtccag
    14701 tcgcaaacct ggcatgtggc ggcgcgaggc gaacatgcgc tggtgattgc accgactggc
    14761 tccgggaaaa cgctggcagc atttctctac gccctcgatc ggctcttccg cgaaggcggc
    14821 gaagataccc gcgaggcgca taagcgtaaa acctcacgca tcctctatat ttcaccgata
    14881 aaagccctgg gcaccgacgt tcagcgcaac ttgcagatcc cgttgaaagg tattgccgat
    14941 gaacggcggc ggcgcggcga aacggaagtc aatcttcgcg tagggatccg tactggcgat
    15001 acgcctgcac aggaacgcag caaactcacc cgtaatccgc cggatattct gatcaccaca
    15061 cccgaatcac tctatctgat gctgacctcc cgcgcgcgcg aaacgctacg cggcgtcgaa
    15121 acggtaatta ttgatgaagt ccacgcggta gcgggcagta aacgtggtgc gcatctggcg
    15181 ttaagtctgg agcggctcga tgcgctgctc cacacctcag cacagcgaat tggcctttct
    15241 gccactgtgc gctcagccag cgatgtggca gcatttcttg gtggcaatcg cccggttacg
    15301 gtagtcaacc cgcccgcaat gcgccatccg cagatacgaa ttgtcgtacc ggtcgccaat
    15361 atggatgatg tctcatcggt cgccagcagc accggcgaag acagccatgc cggccgggaa
    15421 ggctccatct ggccatatat tgaaacgggt atccttgatg aagtgttgcg ccatcgctcg
    15481 accattgtct ttactaattc acgtgggctg gcggaaaaac tgacggcacg attaaatgag
    15541 ctttacgccg cacgcttaca gcgttccccg tctatcgccg ttgatgcggc ccatttcgag
    15601 tcgacctccg gcgcaacctc taaccgtgta caaagtagcg acgtttttat tgcccgctca
    15661 caccacggct ccgtctctaa agaacaacga gcaatcaccg agcaggcgct gaaatcgggt
    15721 gagttacgct gcgtggtcgc aacctccagt ctcgaactgg ggattgatat gggcgcagtg
    15781 gatctgatga ttcaggtggc aacgccgctt tctgttgcca gtgggttaca acgcattggt
    15841 cgcgccggac atcaggttgg cggtgtatct aaagggctgt ttttccctcg tacccggcgt
    15901 gacttagtcg attccgcagt cattgtagag tgtatgttcg caggcaggct ggaaaacctg
    15961 acaccgccgc ataatccact cgacgtcctt gcgcagcaaa ccgttgccgc cgcggcgatg
    16021 gatgcattac aggtagacga atggtactcc cgcgtacgcc gtgccgcacc gtggaaagat
    16081 ctgccaagac gtgtttttga cgctacgctg gatatgcttt ccgggcgcta tccctctggc
    16141 gatttttctg cttttcgccc caaactggtc tggaaccggg agaccgggat attgaccgcc
    16201 cgacctggcg ctcaattgtt ggcggttacc agcggtagca ccattccgga tcgtggcatg
    16261 tatagcgtgt tattacccga aggtgaagaa caggccggtt cgcggcgggt gggtgaactg
    16321 gatgaggaga tggtatatga gtcgcgggtg aacgacatta tcactctcgg cgctacctca
    16381 tggcggatcc agcaaatcac ccgcgatcag gtgattgtga ctcctgctcc gggtcgttct
    16441 gcccggctcc ccttctggcg tggtgaaggt aacggacgtc cggctgaatt aggcgagatg
    16501 atcggcgatt ttcttcattt gctggcggat ggcgcgttct tttccgggac tattcccccg
    16561 tggctggcag aagaaaatac gatcgccaat attcaggggt tgattgacga gcaacgcaac
    16621 gcgacgggca tcgttccggg gagtcgccat ctggtcctcg aacggtgccg tgatgaaatt
    16681 ggcgactggc gtattatttt gcactctccc tatggaagac gggtgcatga accctgggcg
    16741 ctggcgattg ccgggcgaat acatgcccta tggggcgctg acgcgtctgt ggtcgccagt
    16801 gatgacggca ttgttgcgcg cattcctgac actgatggta aattgcccga cgccgcgatt
    16861 tttttgtttg aaccagaaaa gttgctgcaa attgtccgcg aggcggtagg cagctcggca
    16921 cttttcgccg cccgttttcg cgaatgcgcc gcgcgggcat tattaatgcc ggggcgcact
    16981 ccgggccatc gcaccccgct ttggcaacaa cggctgcgcg ccagtcagtt gctggaaatc
    17041 gctcagggat atccggattt tccggtcatt ctcgaaaccc tacgcgaatg tctgcaagat
    17101 gtttatgatc ttcccgcact ggaacgtttg atgcgtcgcc tgaacggtgg cgaaattcaa
    17161 atatccgatg taacgaccac tacgccctcg cctttcgcca caagtttatt gttcggctat
    17221 gtcgcggaat ttatgtacca gagcgacgcc ccgctggcag agcgccgggc atccgtactg
    17281 tcgctggaca gcgagttact gcgcaatcta ctcggacagg tcgatccggg ggaattactc
    17341 gacccgcagg tcattcgcca ggtggaagaa gagttgcaac gactggctcc tggcagaaga
    17401 gcgaaaggtg aagaaggatt gttcgacctg ctgcgcgaac tggggccaat gaccgttgaa
    17461 gacctggcgc aacggcatac aggcagcagt gaagaggttg cgtcgtatct ggaaaatctt
    17521 cttgcagtaa aacgaatctt cccagcgatg attagcggac aggagcgtct tgcctgtatg
    17581 gatgatgccg ccaggctgcg tgatgccctc ggcgtacgac taccagagtc attgccagag
    17641 atttatttac atagagtcag ttacccgctt cgcgacctct ttctgcgcta tctccgggct
    17701 catgctctgg tcacggctga acaactggct catgagttta gtctcggtat tgccattgtc
    17761 gaagagcagc ttcagcaact gcgtgaacag ggtctggtga tgaatctgca acaagacatc
    17821 tgggtgagcg atgaagtatt tcgtcgtctg cgtttgcgct cgctgcaagc cgccagagaa
    17881 gcgacgcgtc ccgttgcagc cacgacctat gcgcgattgc tgctggaacg tcagggcgta
    17941 ttacccgcca ccgatggtag cccggcgctc tttgcctcaa catcgccagg cgtttatgag
    18001 ggcgtagatg gcgtgatgcg ggtgatcgaa cagcttgccg gagtcggttt acccgcctca
    18061 ctctgggaaa gccagatcct gcctgcccgc gtacgcgact attcgtcaga aatgctcgat
    18121 gaattactgg caaccggtgc ggttatctgg tcggggcaaa aaaactgggt gaagatgacg
    18181 gcctggtggc actgcatcta caggaatatg ctgcagaatc gttcactccc gccgaagcgg
    18241 atcaggcgaa tcgttcggcg ctgcaacaag cgatagtcgc tgttctggct gacggaggag
    18301 cctggtttgc acaacaaatc agccaacgga tacgcgacaa aatcggcgaa tcggttgatc
    18361 tctctgccct gcaagaggcg ttatgggcgc tggtctggca aggcgtcatc accagcgaca
    18421 tttgggcacc gttacgcgcc ctcacccgca gcagttccaa cgcacgcacc tcaactcgcc
    18481 gcagtcaccg ggctcgtcgt ggacgtcctg tctatgcgca acccgtctcg ccgctggtat
    18541 cttacaacac accaaatctg gctggatgct ggcgctggcg gaaaatatgc tcgaccgcta
    18601 cggcatcatc agtcgtcagg cggtgatagc cgaaaatatc cctggcgggt ttccatcgat
    18661 gcaaacgctt tgtcgaagta tggaagactc cgggcgaatt atgcaaggtc gttttgtaga
    18721 aggtctgggt ggcgcgcaat tcgctgaacg tctgactatt gaccgattgc gcgatctggc
    18781 gacacaagcc acgcaaacgc gccactatac accagtggcg ctctctgcca acgatccggc
    18841 taatgtgtgg ggaaatcttc tgccctggcc tgcacatccg gcaacgctgg ttccaacgcg
    18901 tcgggcgggt gcgctggtgg tcgtttctgg cggcaaattg ttactctatc tggcgcaagg
    18961 tggcaaaaaa atgctggtct ggcaggaaaa agaggaatta ctcgccccag aggttttcca
    19021 cgcgctgact accgcactgc gtcgcgaacc acggctgcgc tttacgctaa cagaagtgaa
    19081 tgatctaccg gtccggcaaa cgccgatgtt tacgctgctg cgtgaggcgg gattttcaag
    19141 ttcgccacaa gggctggatt ggggatagag aaaggactga cggatgcccg ttcgcatccg
    19201 tcagtattgc aggacggatt attccgcgtc cggctcttca gacttgtatt tagcggcagt
    19261 ttctttgatc agctgctgca gttcgccacg ctgatacatt tcgatcacga tatcacaacc
    19321 gccgaccagc ttgccgtcaa cccacagttg cgggaaggtc ggccagttag catatttcgg
    19381 cagttccgca cgaatgtccg gattctgcag aatatcaaca taggcaaaac gttcgccaca
    19441 tgcggcaagc gcctggactg cctgggcaga gaaaccgcag ctcggcagtt tcggtgaacc
    19501 tttcatgtac agcaggatcg ggttttcagc aatctggcgt tggatttttt cgatagtggt
    19561 gctcattgtc ttgcttcctc aaacttcttt acggcagtaa tctgacattg tagcgggtca
    19621 gtgcggcatc ggaaaataac attttcatca cgcttttgct attttatccc tttgctctat
    19681 ttgttgcatt tcaaatattc gtttcttctc tgatgccggc aaactggcgt atttataacc
    19741 atttttagct ttcactgctg catttttttg cgctcgccaa cgaaacgtat tttttaacaa
    19801 taaaagctat taactttctc ttcttctatg cattagaatc atcaagtttt gtaaatcaga
    19861 cgcaggcatg atagacctgc cttaacagag ggacgctcag tggcgcggat aaaccgtatt
    19921 tcgatcacgc tctgtgcttt actttttacc accctgcctt taacgcctat ggcccatgct
    19981 tcaaagcaag ccagggagag ttctgctacc actcatatca ccaaaaaagc agataaaaag
    20041 aaaagcacgg caaccaccaa aaagacccag aaaacagcga aaaaagccgc cagtaaaagt
    20101 acgaccaaaa gcaaaaccgc ttcttccgtt aaaaaatctt ccattaccgc ttctaaaaac
    20161 gccaaaactc gcagcaaaca cgccgtcaat aaaacggcct cagccagctt taccgaaaag
    20221 tgtaccaaac gtaagggcta taaatcgcat tgtgtgaaag tcaaaaatgc cgcgtcagga
    20281 actcttgccg acgcgcacaa agcgaaggtg caaaaagcta cgaaagtggc aatgaataaa
    20341 ctgatgcagc aaattggtaa gccatatcgt tggggtggca gctcaccgcg taccggtttt
    20401 gattgcagcg gcctggttta ttacgcttat aaagatttgg tgaaaattcg tattccgcgt
    20461 acggcgaatg aaatgtatca cctgcgtgat gcagcgccaa tcgaacgtag tgaactgaaa
    20521 aacggcgacc tggtcttttt ccgtactcag ggacgcggca cagccgatca tgtcggcgtg
    20581 tatgtcggca acggcaaatt tattcagtca ccgcgcacag gtcaggaaat tcaaatcact
    20641 tctctcagtg aagactactg gcagcgccac tatgttggcg ctcgtcgggt aatgacccca
    20701 aaaacacttc gctaaaactt taccctgttg ttacggcaac agggtaagtt catcttttgt
    20761 ctcacctttt aatttgctac cctatccata cgcacaataa ggctattgta cgtatgcaaa
    20821 ttaataataa aggagagtag caatgtcatt cgaattacct gcactaccat atgctaaaga
    20881 tgctctggca ccgcacattt ctgcggaaac tatcgagtat cactacggca agcatcatca
    20941 gacttatgtc actaacctga acaacctgat taaaggtacc gcgtttgaag gtaaatcact
    21001 ggaagagatt attcgcagct ctgaaggtgg cgtattcaac aacgcagctc aggtctggaa
    21061 ccatactttc tactggaact gcctggcacc gaacgccggt ggcgaaccga ctggaaaagt
    21121 cgctgaagct atcgccgcat cttttggcag ctttgccgat ttcaaagcgc agtttactga
    21181 tgcagcgatc aaaaactttg gttctggctg gacctggctg gtgaaaaaca gcgatggcaa
    21241 actggctatc gtttcaacct ctaacgcggg tactccgctg accaccgatg cgactccgct
    21301 gctgaccgtt gatgtctggg aacacgctta ttacatcgac tatcgcaatg cacgtcctgg
    21361 ctatctggag cacttctggg cgctggtgaa ctgggaattc gtagcgaaaa atctcgctgc
    21421 ataataactg atggcaaatg cagcattgcc tgatgcgctt cgcttaccat gccaacgcgg
    21481 atgaggcgtt aacagcgcat tcggcaagat aaatcgcacg ttgtcagcaa ctgtaacgca
    21541 gaaggttatc cttctgcgtt tttgtttaat tagctgttgg caacgcaaac tgtttcaggt
    21601 tgttttctgg ctgacataaa caccagcaat aatgccagtc ccgcgacaat cgcccccatc
    21661 accggcacaa agctgtatcc cagcccagcg gaaattaccg caccaccagc cgctgctccc
    21721 agcgcatttc caagattaaa ggcaccaata ttgactgatg aagacagacc tggcgcttca
    21781 ctggcgacac gcatcacgcg catctgtaac ggcggtacga ccgcaaaggt tgctgcgccc
    21841 cacaccacca tgctaatagc tgcgccgaac tcattgcggg ccaggaacgg ggttgccagc
    21901 ataatcacca tcagcagcaa caaaaagcct ttcaacgtgc cgttaactga acgatctgcc
    21961 agtttgccgc cgagatagtt accgatagag aatccgacac caatcagcac cagcattgcc
    22021 gtgacgaaca ccggtgttgc gtgggtaata ctttgcagta ccggagagat ataggtgtag
    22081 agagtaaaca ttgcgccagc tcccagtacc gtcgtcagca atgcaggcag cacctgcgga
    22141 cgcattaata ccgccagctc ttttttcact tcaggtcgtg cccctgtacc acctttaggt
    22201 aatgagaaga acagacttac cattgaaatc actcccagcc ccgccgttgc cagaaatgac
    22261 atccgccagc cgatggtttc acccaaccag gtcgccgccg gcacgccacc gatatttgcc
    22321 agggttaacc ccataaacat agtggcaact gcgctggcct gtttatgttt tggcaccacg
    22381 cttgcggcca cgactgaacc caaaccaaaa aatgctccgt gattcaggct ggtcaaaatg
    22441 cgtgaaagca tcagggtcat ataatccggc gcgatggcgg aaagtacgtt gccgagcgtg
    22501 aaaattgcca tcaggaaaat caacgcactg cggcgggcac gatgagaaag tagaagcgtc
    22561 atcagcggcg cgcccaccat tacgccaact gcataggcac tgattaacat tccggcagcg
    22621 ggaatcgaga catccacacc gcgcgcaatg acgggcaaca agcccattgg cgagaactcc
    22681 gttgtcccga taccaaacgc gccaatcgcc agcgccagca acggatagtt aattttcatg
    22741 ccttatctcc acctcttcgc gtcattacgc gatattcatt aaagtggcga aagcatgaca
    22801 gcaatcacaa aaaaatgaaa ataacaaaaa gagaaaacac ttttgccatt ttgctaacaa
    22861 acaggaagga gatgcgaggg agaacgcgct ccctcgagag gaaatcagtg cagcgcggca
    22921 gtcaaaccca cggctacgat caaaccgagg acgataatcg ttgttaccag tgaaaattta
    22981 aggtcggtgc tcatcaagtt ttctcctttt ttattaccac acaaaaagtg atattacgca
    23041 tttttacaca ctgtgatgaa aaaatctccc gtcatttata atgataagtg tttttaccac
    23101 ttcccctttt cgtcaagatc ggccaaaatt ccacgcttac actatttgcg tactggccat
    23161 tgaccccttc ctgacgctcc gtgtcgtttt tccggcgtac cgcaacactt ttgttgtgcg
    23221 taaggtgtgt aaaggcaaac gtttaccttg cgattttgca ggagctgaag ttagggtctg
    23281 gagtgaaatg gaatggcaac aataaaagat gtagcgaaac gagcaaacgt ttccactaca
    23341 actgtgtcac acgtgatcaa caaaacacgt ttcgtcgctg aagaaacgcg caacgccgtg
    23401 tgggcagcga ttaaagaatt acactactcc cctagcgcgg tggcgcgtag cctgaaggtt
    23461 aaccacacca agtctatcgg tttgctggcg accagcagcg aagcggccta ttttgccgag
    23521 atcattgaag cagttgaaaa aaattgcttc cagaaaggtt ataccctgat tctgggcaat
    23581 gcgtggaaca atcttgagaa acagcgggct tatctgtcga tgatggcgca aaaacgcgtc
    23641 gatggtctgc tggtgatgtg ttctgagtac ccagagccgt tgctggcgat gctggaagag
    23701 tatcgccata tcccaatggt ggtgatggac tggggtgaag caaaagctga cttcaccgat
    23761 gcggtcattg ataacgcgtt cgaaggcggc tacatggccg ggcgttatct gattgaacgc
    23821 ggtcaccgcg aaatcggcgt tatccccggc ccgctggaac gtaacactgg cgcaggccgc
    23881 cttgtcggtt ttatgaaggc gatggaagaa gcaatgatca aggtgccgga aagctggatt
    23941 gtgcagggtg actttgaacc tgaatccggt tatcgcgcca tgcagcaaat cctgtcgcag
    24001 ccgcatcgcc ctactgccgt cttctgtggt ggcgatatca tggcaatggg cgcactttgt
    24061 gctgctgatg aaatggggct gcgcgtcccg caggatgttt cgctaattgg ttatgataac
    24121 gtgcgcaacg cccgctattt tacgccggcg ctgaccacaa tccatcagcc aaaagattcg
    24181 ctgggtgaaa cagcgttcaa catgctgttg gatcgtatcg tcaacaaacg tgaagaaccg
    24241 cagtccattg aagtgcatcc gcgcttgatt gaacgccgct ccgtggctga cggcccgttc
    24301 cgcgactatc gtcgttaatc acccgttgcg ggagtctctt ccggctcccg cagccactcc
    24361 ttattcagcg tctcactatc gccgagatac tcaagcaacc aggttaacgc aggcgacata
    24421 tcattttgct gccatgtcag acaacatgcc gaatccggaa aggggttttc cagttcaagt
    24481 gctacccact tcccctcatt aagccacggt ttggcgaaat gtgttggcac catccctatg
    24541 cataatcctg ccgagataca ggttgccgat gattcccagt caggcacgac aactcttttt
    24601 tggttatcca gcaaccaggt aatacgttta ggtagcgttc gcgaggtgtc ttcgcgcacc
    24661 aacgacggcc agttgcgcaa cgtatcatcg ctgaacgggc catccatcaa cgccagcggg
    24721 tgatggctgg caacaacgca actccagctt agcatcccca tatcccggaa ggcataacga
    24781 ccgcctaccg gaatcgcgcg tgttgcgcca atcgccagtt ccacgcgccc gtcggaaagc
    24841 gcatcccaga caccgttgaa cacttcctga aagacaagaa gttcgacatc atcaaaatgg
    24901 cgataaaaat caacgatcat ctgccgtgta cgttctggcc tgacaatatt atccactgcg
    24961 atagctaact gaccgcgcca gccgttcgct atctgctgac attgctggcg ggtgatctgc
    25021 atttttttga caacagagcg cccttctttg agaaaccacg ctccagcagc ggtcagctcc
    25081 acatcacggt gccgtcgttc aaagagcggc accgccagcc actcttccag ctgacgcacg
    25141 gtatagctga ccgcagaagg aacgcgatgc agctcctgtg ccgcagcgct aaaactacca
    25201 ttacgcgcta ccgcatcaac aacttcgagt gaatattctg accacatagt ctgcctgcaa
    25261 aatttttgaa accaatcatc aaatattacc gtttcacaac actaatttca ctccctacac
    25321 tttgcggcgg tgtttaattg agagatttag agaatataca tgcaacctgg gaaaagattt
    25381 ttagtctggc tggcgggttt gagcgtactc ggttttctgg caaccgatat gtatctgcct
    25441 gctttcgccg ccatacaggc cgacctgcaa acgcctgcgt ctgctgtcag tgccagcctt
    25501 agtctgttcc ttgccggttt tgccgcagcc cagcttctgt gggggccgct ctccgaccgt
    25561 tatggtcgta aaccggtatt attaatcggt atgacaattt ttgcgttagg tagtctgggg
    25621 atgctgtggg tagaaaacgc cgctacgctg ctggtattgc gttttgtaca ggctgtgggt
    25681 gtctgcgccg cggcggttat ctggcaagcg ttagtgacgg attattatcc ttcacagaaa
    25741 gttaaccgaa tttttgcgac catcataccg ctggtgggtc tatctccggc cctggctcct
    25801 ctgttaggaa gctggctgct ggtccatttt tcctggcagg cgattttcgc caccctgttt
    25861 gccattaccg tggtgctgat tctgcctatt ttctggctca aacccacgac gaaggcccgt
    25921 aacaatagtc aggatggtct gacctttacc gacctgctac gttctaaaac ctatcgcggc
    25981 aacgtgctga tatacgcggc ctgttcagcc agtttttttg catggctgac cggttcaccg
    26041 ttcatcctta gtgaaatggg ctacagcccg gcagttattg gtttaagtta tgtcccgcaa
    26101 actatcgcgt ttctgattgg tggttatggc tgtcgcgccg cgctgcagaa atggcaaggc
    26161 aagcagttat taccgtggtt gctggtgctg tttgctgtca gcgtcattgc gacctgggct
    26221 gcgggcttca ttagccatgt gtcgctggtc gaaatcctga tcccattctg tgtgatggcg
    26281 attgccaatg gcgcgatcta ccctattgtt gtcgcccagg cgctgcgtcc cttcccacac
    26341 gcaactggtc gcgccgcagc gttgcagaac actctacaac tgggtctgtg cttcctcgca
    26401 agtctggtag tttcctggct tattagtatc agcacgccat tgctcaccac caccagcgtg
    26461 atgttatcaa cagtagtgct ggtcgcgctg ggttacatga tgcaacgttg tgaagaagct
    26521 ggctgccaga atcatggcaa tgccgaagtc gctcatagcg aatcacactg atctatatcg
    26581 atatacttat acttaggctg ctaacaaaat tttgttgtat gattgaaatt agcggcctat
    26641 actaatttcg agttgttaaa gctacgataa atattatgtt tttacgggga caggatcgtt
    26701 cccgactcac tatggatggt catttcggca agggttcctc ctttccctct gttctacgtc
    26761 ggattataga ctcgcggttt tttctgcgag atttctcaca aagcccaaaa agcgtctacg
    26821 ctgttttaag gttctgatca ccgaccagtg atggagaaac tatgagttca tcgtgtatag
    26881 aagaagtcag tgtaccggat gacaactggt accgtatcgc caacgaatta cttagccgtg
    26941 ccggtatagc cattaacggt tctgccccgg cggatattcg tgtgaaaaac cccgattttt
    27001 ttaaacgcgt tctgcaagaa ggctctttgg ggttaggcga aagttatatg gatggctggt
    27061 gggaatgtga ccgactggat atgtttttta gcaaagtctt acgcgcaggt ctcgagaacc
    27121 aactccccca tcatttcaaa gacacgctgc gtattgccgg cgctcgtctc ttcaatctgc
    27181 agagtaaaaa acgtgcctgg atagtcggca aggagcatta cgatttgggt aatgacttgt
    27241 tcagccgcat gcttgatccc ttcatgcaat attcctgcgc ttactggaaa gatgccgata
    27301 atctggaatc tgcccagcag gcgaagctca aaatgatttg tgaaaaattg cagttaaaac
    27361 ctgggatgcg cgtactggat attggctgcg gctggggcgg actggcacac tacatggcat
    27421 ctaattatga cgtaagcgtg gtgggcgtca ccatttctgc cgaacagcaa aaaatggctc
    27481 aggaacgctg tgaaggcctg gatgtcacca ttttgctgca agattatcgt gacctgaacg
    27541 accagtttga tcgtattgtt tctgtgggga tgttcgagca cgtcggaccg aaaaattacg
    27601 atacctattt tgcggtggtg gatcgtaatt tgaaaccgga aggcatattc ctgctccata
    27661 ctatcggttc gaaaaaaacc gatctgaatg ttgatccctg gattaataaa tatatttttc
    27721 cgaacggttg cctgccctct gtacgccaga ttgctcagtc cagcgaaccc cactttgtga
    27781 tggaagactg gcataacttc ggtgctgatt acgatactac gttgatggcg tggtatgaac
    27841 gattcctcgc cgcatggcca gaaattgcgg ataactacag tgaacgcttt aaacggatgt
    27901 ttacctatta tctgaatgcc tgcgcaggtg ctttccgcgc ccgtgatatt cagctctggc
    27961 aggtcgtgtt ctcacgcggt gttgaaaacg gccttcgagt cgctcgctaa aggctattct
    28021 atcgccccct ctccgggggc gatttcagat caggcttctg tacctggttg attcatggca
    28081 ttttctcgtg ccgccagcac acgttctacc gtatctacca ctgcctgagt ttgtggatca
    28141 atttcaatgt tgacgcgtgc gccgagtttt ttcttcccaa gagtcgtgcg ttccagagtt
    28201 tccggaatta aatggacgca aaaacgcgtt ggcgtgactt cgccgacggt caggctaata
    28261 ccgtcgatgc caataaatcc tttgtacagg atatatttca tcaactgact atcctggact
    28321 ttaaaccaga tctggcgatt attttccgag gttaatattt tcgccacttc agcggtggtc
    28381 ataatatgac ctgacattaa gtgtccgcca atttcatcgc tgaattttgc cgcacgctca
    28441 acgtttaccc aatcccccac ttttaaatcg ccaagattgg taatgcgtaa cgtttctttc
    28501 atcaggtcaa aactgacatg gttgccgtta atttccgtca ctgtcaggca gcaaccgtta
    28561 tgcgccacgg aagcacctgt ttccaggccg tccagcatgt ggtcgggtaa ctccaccaca
    28621 tgcgtacgaa aatttggttt ctcgtcaatc gacaccagtt ttgcggtgcc ctgtacaatc
    28681 cccgtaaaca tacttacaac tcctgaaatc agttaagaca ttctgttcag cacaatagca
    28741 ggtggaaaac gcccttacca gtgaaggggt aagaatgact attttttcac tggagaatta
    28801 ataaatcctc gctacaatag actgaatttc ccctgcttct tctttttgct gcccattcag
    28861 gcggcttttt agtctctcat ataactacaa ataaaaggtg ttcacgtgca gaagtatatc
    28921 agtgaagcgc gtctgttatt agcattagca atcccggtga ttctcgcgca aatcgcccaa
    28981 actgcgatgg gttttgtcga taccgtgatg gcgggcggct atagtgccac cgacatggcg
    29041 gcggtcgcta tcggtacttc tatctggctt ccggcgatcc tctttggtca cggactgctg
    29101 ctggcattaa cgccggttat cgcgcaatta aatggttccg gtcgacgtga gcgcattgcg
    29161 catcaggtgc gacaaggttt ctggctggca ggttttgttt ccgttctcat tatgctggtg
    29221 ctgtggaatg caggttacat tatccgctcc atggaaaaca tcgatccggc tctggcggac
    29281 aaagccgtgg gttatctgcg tgcgttgttg tggggcgcgc caggctatct tttcttccag
    29341 gttgcccgta accagtgtga aggtctggca aaaaccaagc cgggtatggt aatgggcttt
    29401 atcggcctgc tggtgaacat tccggtgaac tatatcttta tttatggtca tttcggtatg
    29461 cctgagctcg gtggcgttgg ttgtggcgtg gctactgcgg cggtgtattg ggttatgttc
    29521 cttgccatgg tttcttacat taaacgcgcc cgctccatgc gcgatatccg taacgaaaaa
    29581 ggcaccgcaa aacccgatcc tgcggttatg aaacgactga ttcaactcgg tttgccgatt
    29641 gcgctggcac tattctttga agtgacactg tttgccgtcg tggctctgtt agtgtctccg
    29701 ctcggtattg ttgatgtcgc aggacaccag attgccctga actttagttc actaatgttc
    29761 gtgcttccaa tgtcgctggc ggcagcggta actatccgcg taggttatcg tctgggtcag
    29821 ggctcaacgc tggatgcgca aaccgctgct cggaccgggc ttatggtggg tgtctgtatg
    29881 gcaaccctga cggccatttt cacggtttca ctgcgggagc aaatcgccct gttgtacaac
    29941 gacaatcccg aggttgtaac gctggctgcg catttgatgt tgctggcggc ggtatatcag
    30001 atttctgact caatccaggt gattggcagt gggattttgc gtggttataa agatacgcgt
    30061 tccattttct atatcacctt tacagcttac tgggtgctgg gcttgccaag cggctatatt
    30121 ctggcactga ccgatctggt cgttgaacct atggggccag caggcttctg gataggcttt
    30181 attatcggcc tgacatcggc agccattatg atgatgctgc gtatgcggtt cctgcaacgt
    30241 atgccgtcag ccatcattct gcaacgagcg tcccgctaat aaagacaagg cgcaaccttc
    30301 acgggttgcg cctgtgtttt tacgcaggct ggagcgttgc gccaatcccg tcttcgtctg
    30361 gctgtaattt cagagcgtta cacagagttt gccgactggc atctgttatc tcaacgcgta
    30421 tatccccgtt aacttcccct ttcacattga tgaagtcagg tgaaatttct ttcgcactgg
    30481 tttgcaggca aatcgctccc tctccgcgca ggcgtttcac tttcagttgc acatgcgttg
    30541 ccgcaccagt gatcagccac agttttccgc cattcagatt gatgttacca acaatgaaag
    30601 tgacgctgtc accttttacc gtactgccgt cgagaatcaa cacaccgccc tgctgtacag
    30661 taatcgttga ttgttgcagc tctgcgccat aaccaataat cattgaagcg ccgtccagta
    30721 atgcaacact cccggtcagt tcaaccggtg cgcgatcttc ctctacgaca aaattttcgt
    30781 ccgcgctggt gggtgacaac aggatcactg ttccaccttg tattaccgca ttttccagcg
    30841 taccggcatg cacttcagcc cgccccccca cattcaccga caggttttgc gtcttaccgg
    30901 aactgtagag ctgaaggcca tccgtcccca gacgataaat ggatccattc tcaacaatag
    30961 tgtcagagtc ttcaccaccg agattattca tggtgccacc agcaagcaac gtatcactgg
    31021 ctttcccggc cagcataaaa acgccgccct gattaatctc acaattgctg gcttctccac
    31081 ttggatacac caacaattca ccaccttcat ctaccacgac cgctgaggtt gtcccattaa
    31141 ccaacaggcc gccctcttga tcgagaatga ttttttccgc gcgatgtcct tccagtacac
    31201 gcaggttacc gccattttcc agcaacaaac cgcaggcata accctgatca acgctgaatt
    31261 caccttcggg atggcggcca ttaaccgtag cgagcgtaga gagactaatt gcgcctccct
    31321 cggactgaac aatatctgtg gcaatggcac cgtcgctgac cgatagcgtg ccattgttac
    31381 cgtactgtgt gcatttggcg atcccaccgg ttgccacttt caaccagcca gcccccgtga
    31441 ccacggtatt ttcggctctt ccgccagctt caacaatcaa ctcgccttca gtaagttgcg
    31501 tcgcgccaat gacctcgccg gtcttaacaa tttgcacatt cccgtcgctc atcaagtttt
    31561 tcgcatcaga tcccatgatt tattcctttg ctgcatctgt gtgcctttat tgctacctaa
    31621 gtgtaaaggc tacggaggat ttatccacga cagatttgag atggtggcaa acaattctgt
    31681 ttaaactctg atacacgaat tattgggttg tatcagatgt aaatgcgatc ctgaataaaa
    31741 atcgcccttg caaatcaaca aaatatagcc aatttgctta aaagctctgc aaacggtcga
    31801 aatagcgcag aaaattacgt tttgcctctt gccacctttc cactctgccg ctaatatttg
    31861 tccccgttgt cacctacaac gttgcgttca tagctcagtt ggttagagca ccaccttgac
    31921 atggtggggg tcgttggttc gagtccaatt gaacgcacca tcctgcgtcc gtagctcagt
    31981 tggttagagc accaccttga catggtgggg gtcggtggtt cgagtccact cggacgcacc
    32041 agattttctt aatctggtct tttccttttt ccctctgttt attctctgta tccaatacgt
    32101 taaaagattt acactgtctt cgtatgcgtt atcagaagga gaatcgctat ggcaactttg
    32161 ttacaacttc attttgcttt taatggccca tttggcgacg caatggctga gcagcttaaa
    32221 ccacttgctg agtcgattaa tcaggaacct ggttttctgt ggaaggtatg gacagaaagt
    32281 gaaaagaacc acgaagccgg tgggatctac cttttcactg atgaaaaaag cgcccttgcc
    32341 tatctggaaa aacatactgc ccgactgaaa aatctcggcg ttgaggaagt tgtcgccaaa
    32401 gttttcgatg tcaatgagcc acttagtcaa atcaatcagg caaaactcgc ctgacagaat
    32461 ttaatcaagg gcggttagcg cccttttcat ccctgtctga aatttctcaa attctaaaaa
    32521 tctcaaccaa acttatctga taacactaaa ttcgaaagaa tgcgtacagg taagtaacaa
    32581 tgaaaaaaat tgctattgtg ggtgccgggc ctacggggat ctacacctta ttctcgcttc
    32641 tacagcaaca aactccactt tctatttcta tcttcgagca ggctgacgag gccggtgtcg
    32701 ggatgccata cagtgatgag gaaaactcaa aaatgatgct ggcaaatatt gccagtattg
    32761 aaataccgcc gattaattgt acgtatctcg aatggctaca aaagcaagaa gccagccatc
    32821 tccagcgtta tggcgttaaa aaagaaacct tgcacgatcg tcagttttta ccgcgaattc
    32881 tgctgggcga atatttccgc gatcaatttt tacgattagt agaccaggca cgacagcaaa
    32941 aatttgcagt ggctgtttat gaatcatgcc aggttaccga tctgcaaatt acaaatgctg
    33001 gcgtcatgct cgctacaaat caggatttac ccagcgagac gtttgattta gcggtgattg
    33061 ccacgggtca cgtctggcct gatgaagaag aagcaaccag aacgtatttt cccagcccgt
    33121 ggtcaggcct gatggaagca aaggtcgatg cgtgtaacgt gggtattatg ggaacatcct
    33181 tgagcggact ggatgcggca atggcagtgg ctattcagca tggttcgttc attgaagatg
    33241 ataaacaaca cgtcgttttt caccgcgata acgcaagtga aaagctaaat atcacgttga
    33301 tgtcgcgcac gggtatttta cccgaagccg atttctattg ccctattccc tacgagccct
    33361 tacacatcgt caccgatcag gcattaaatg ctgagattca aaaaggcgaa tatggccttt
    33421 tggatcgggt atttagattg atagtagagg aaatcaagtt tgctgatcca gactggagcc
    33481 aacgcatagc cttagagagc ctgaatgtcg attcctttgc tcaagcctgg tttgccgagc
    33541 gcaaacaacg cgacccattt gactgggcag aaaaaaatct ccaggaagtc gaacgcaata
    33601 aacgagaaaa acatactgtt ccctggcgtt atgtcattct gcgcctgcat gaagccgtac
    33661 aggaaattgt tccacatctg aatgaacacg accataaacg gttcagtaaa ggccttgccc
    33721 gggttttcat cgataattat gcggcaatcc cttcagagtc tattcgtcgg ctgctggcct
    33781 tacgtgaagc ggggatcatt catattctcg ccctcggtga agactacaaa atggaaatta
    33841 atgagtcgcg caccgtcctg aaaacggaag acaacagcta ctcgtttgac gtttttattg
    33901 atgcccgcgg acagcgtccg cttaaagtga aagatattcc tttccctggg ctacgcgagc
    33961 aattacagaa aacaggggat gaaatccctg atgttggcga agattatacg ttacagcaac
    34021 ccgaagatat tcgtggacgc gtagcgttcg gcgcgttgcc ctggttgatg cacgaccagc
    34081 ctttcgttca gggacttacg gcatgtgcag aaattggtga ggcgatggct cgggcggtcg
    34141 taaagcctgc atcccgtgct cgtcggcgtc tttcgtttga ttaaaggtaa agctatcagc
    34201 actcaacaaa ctgatgctgt tcattaagac gatagcggac tccggttcga atattgtttt
    34261 caccttcaat ggcgacggcg aagcgaactc tttcgccgtc atgatatgcc agtgcggcgc
    34321 ttccccctgg cccgagaata attgagtcga ccacgcccgt gctcactacc gttgaatttt
    34381 caccgctggc aatgatgtgt acattgtcgc cgctgttggc aattctggcg tttgcgccaa
    34441 agctggcgat ttgcaccagg tcaccattac tggcgacatg acaccgttca cccaatgtac
    34501 aaacgcgtac tcgcattccg gtattcgcaa tccgactact atctccagcg ctgcttatcc
    34561 gcgaagaatt accggttaca gcaacccgtg cgcgttcacc ggagctgcca atgtgactgt
    34621 taaagccgac acttccaatc cgcacgctat aacccatact ggcaatttgc gcggcatacc
    34681 cagcggttac gattcgggcg ttatcctcac aacagccgat gcgagcatga tcgtcgctgc
    34741 gggcaaactg actgcaaaat ggtatctctc cggggtctgt ggccagtttt tgcatagagg
    34801 aaacttcctg atgcgcgaag ttttcatccg ccagccattt tttccacgca tactcaacca
    34861 gactctccgc ccagtccgta tatccttcct caatcagtgc atgatgaata tcggcataac
    34921 taccgccgcg tgggaaatgg cgcaggaacc agcgatacat caccgctccg atacgccatt
    34981 ctcgcaggtc tgctcgtgta atgatcattc gttagtgccg gtgatagcca tcgaccatgc
    35041 ttttaaaggt ttcgtgtggc gtacgccccg tggtgcaaag ataaagatgt ccgaagataa
    35101 aaaagaggct tataaatgcc agagcaaaat gcgcctgcaa taaccagtat cttacgccag
    35161 gaaacacatc tcccacggct tgcggataga gacacaacag ccccgtcaat agtaacaacg
    35221 gcagcaatcc acacatgaca ccaacatagg cgacctgctg taagggatta aatttagact
    35281 gtgttgttgc cgggaaagga tgttcttccc cctgcataat gccaaacaaa taaaatcgtg
    35341 tttgttttgc cgctcgttcc agccacccct gacgacgaat gcgatagtgg tgaccattat
    35401 ccccaacggc attgatcagc acaaagccga gccagcatgc cagtaacaaa aatccgcaaa
    35461 cttcatgcac cgcaaccaga cttttaaccg cagttgcgcc caccatcgca aagtgattta
    35521 tcagcccact tgccagcaac aatacaaaga gtaacgcatt cgaccagtgc cataatctga
    35581 ccgctttgct gtaaagataa accttctcac catgatcggt ggctgatttc ttcacgccac
    35641 gagcacgaag caaggcgtgc agccccaaca ccaaccacat cccaaccagc aatactccgg
    35701 caatgatcaa ccacaccggc caaaattcgg gagtgaactg tggcacatag ttcgctaact
    35761 ggctctgaaa ctgttcagca tgttgcgacg ggttcataca ttttcctttt taatcaaatg
    35821 ttgaccgaac cggcgataca gatgcggttt cccggcacca agtagctgat attgatagta
    35881 tttattttcc tgcaaccacg cctgaatttc tgggctatct tcgcgaccaa agatcaatgc
    35941 gtgttccgga caggcgctga cgcaaatggg cggaaagcct tttgccagtc gggactcagc
    36001 gcagaaatca catttatctg ccactttagt gacagggtta aggtagcgaa cctggtacgg
    36061 acaagcgcca atgcaataac tgcagccgat gcactgcgat ttttctaccc gcacgatccc
    36121 ctgctcatca cgccacgacg cccctgtcgg gcatacgtcg atgcacggag catcttcgca
    36181 gtgctgacat gactggcgaa aaaagtgata ctgcgtttcg ttgtcgttat cggtgactgg
    36241 aatgtgtgct atcgataaac ggcttccctg agcggggacg tggttagttt tacggcatgc
    36301 gcgggcgcaa atattacagc cattgcaccg tgactcgtca tgaatcatgg cataccgaac
    36361 ttccttttct tgcctcgtgt tcgctaacag agaagacgca gaaccagtaa aaaagattac
    36421 tgttcccatc cccagaacaa atttgcgtcg agtgaaggac atctgattat cccctaatgc
    36481 agtggctgca actgtccacc ctatccatga catctccctg aaatttcagg ggttctgaaa
    36541 ctgctctacc tcgaaccatt cgcataagcc ataagccttg ccataggtca taaatcgctc
    36601 gctaaacaag tacaacaccg ccgtcgatga ctccacctgc cgtatatcgg catattcagg
    36661 tgcaacatcc atcgcggcga tcgcggcttc aatctgcgct tcctggaagt aatatggcgc
    36721 ttgcatcagc atggcgactt tgtaaggacg aggatacgtc tggcactcaa aacgaacggc
    36781 gtgtgcaatg gcgcgacaaa tgtcctgctc cacaacttgc aaagacatcg ccgcgtagtt
    36841 ttcactcatg gcctgcgttg agtaatagta atcatcctga cttcccgtca tacaagcgat
    36901 atccgcgtag tctccgttct gcttaatgct ttccagcaaa actgacagtt cctcttctgc
    36961 aaccgaatag ggtggcgaga gaaacacggt gcgcgccacc agctgcccag ccgcagaatg
    37021 ctggcgaatg aactcagcca ggatttcagc aggttccggt tcggttaccg tatcaacggg
    37081 gtgacgcatc ccctgcaacc attgccgctt cgcctcgtca acttcgctga ctttcgctaa
    37141 cggcaattca tcctgatggt tcatcttacc cattccttac gcaggcagta gattgtgtgc
    37201 tgccagatcg gcggcaatat cttccagccc cagccgctgc aatgtttcgc gggttggaca
    37261 accaagctgt gggtcccagc ccatttcttt gtagaacatc gtgagcgaag catgcatatc
    37321 gtcacgatcc attttgtcag taccttcagt aaagaccggg atctgcggat ccttgtcgaa
    37381 aacccaggaa cagataagat cgtgttcatt acgcatatct ttggtttgca tcagttttac
    37441 cgtgtaggca cgatgcaacg taaagatacg ctctgcagct aaatctaatt tttcctgagt
    37501 catctcttcg ccggtgatcg ctttgaagaa tttggcttca agcgccagat cgccccggta
    37561 attacggctt ttcagcggcg aaacggtcat tggccagacc cagttgcaca gggtgacggc
    37621 gttatgcaaa cagacccgca acagcgacca ctttgcatat ttaatttttg cgtcattgat
    37681 tggcgtgtag tttttggttt catcgtaagc atcttcagaa ccaaaaagtt ctttcgccac
    37741 ttcacgttgc agtttcaatg gcaagccgga accaataaag ttgatatggg tatgcgtcat
    37801 gcagtcacgg ttgaacatac agttaacaat ggaaccgacc tgcgctgacg cttcattggc
    37861 atggtgaacc ggatagccaa acggcgacca gagtttattt ttcgcgtagc cccagtactc
    37921 ttcacccaaa ttccagcgtt ctgcgatggc atatgaacca tcagccaggt gactcagctc
    37981 acccacacga tgcgccagac ggtagtaaaa atctttaatt ttaatgaagt taacgtcacc
    38041 cgcttccagt tgatcccagc gaatttccgc atactcttca gcaggcagaa cacgcttgaa
    38101 cacacctttg ctgtaacaat aggtaaagtc gcgatgcaac tgcccgtagt tacaccatag
    38161 gccgtagtca tcgaacagat tcagacccac caggttacca atcacacgac catcgtcttt
    38221 atcttcaaaa tctttcggac cgttcgggaa gatggtggta tggacaaagt tggctacaca
    38281 agtgttacca cctgtgctgg gcacgccaaa ctctttcacc cgaggaatat tcatttgggt
    38341 catacaacgg atcgggcaag agtggcagcc actcattttt actgtgtatt tctctgccgc
    38401 cggacctaag tcaaaaacgg atttataggt acgaaagccg accgtgttct gattgcctgg
    38461 cggaatttcc cccgtttcaa tcggaccacc ttcagccgcg ccccaaaaca gccctttacg
    38521 tgctgtccaa cgtgacttgg gatctgaata ctctgcccac gattgcggcg tacttggcac
    38581 gacatggttg ttattcgcac caataagttc agtcatcatg taatcgttga gacgcttcat
    38641 ctcctgacga tcggcaatgt tgaccccttt cgtcccttca accgcaatcg ctttcaggtt
    38701 tttcgaaccc attattgcgc cagttcccgc accgccgctg tggttacggc tattcaacat
    38761 gccagagaga ggaacaaggt tttccccagc ctgaccaata gccgccacac aggtttccgg
    38821 actggtcaat cgacaaattt cttccgtcgt tgcgcgcgtc ccttttcccc ataagaaatc
    38881 ggctttttcc aggctaactt tgtcatcttt aatcttcagc cataccggtg atttcgcctt
    38941 cccttcgata ataatgacgt cgtatccagc gaatttcatt tgcgctgcaa aaaagccacc
    39001 catatgggca tcgacgacta aatttccttt ggtaaaagta gaaagtgagg tgatatttac
    39061 gcgagaacta cagggggcac cagatccagt taatgggccg gtagcaaaga ctaatttatt
    39121 cgcttcatcg aaaggtttcg tgcctggcgg tacttcgtca tacataattt tgtagccgaa
    39181 gcccatgcca ccgacaaaac ttttaaactt actggaatct tcgagggtaa tatttcctgt
    39241 cgtgagattg actcttaata tattacctgt ccaaccgtta gccatgattt tttcctttgc
    39301 aagattcaca cagtaatatc tttccactcg ataattttta acgcccctgt tgggcaggcg
    39361 tttgcgcatt caccgcataa cacacatttc gaggattttt tactttcggt atttacggtg
    39421 gccatcatcc acgggcatgc cgtagtacag gcgctacagc caatacagcg tttatgatcg
    39481 acggtaatac agccttcttt ctgctgccag gtaatcgcac caatcgggca gacgttcatg
    39541 cattgcggct ctttgcattg acggcaggtg tccgcggtat agttgagatc gccatacagg
    39601 ccgccgccag agccaacccc gttgtcgcca aagaaataat tgcgatggat tttgatacgg
    39661 gagaagaatg tccctactga gccatcgttg aagttggtac aagagatttc acagcggtgg
    39721 caacccgtgc atcgcgctcg ctgggtcacc agcacccctt tgggtgtatt tatcaaccct
    39781 actgtgccgc tatcaatatc ttcctgtttg caacccaaaa gtgacagtaa cgcaggggca
    39841 attgttagac cagcaaggcc ttttcctgat atacgcaaga attctaatcg cgttaagcca
    39901 atatctaata gtggacgatc aaccgggttc atttatttcg catcctcttc gcaaacacag
    39961 tgctgacaat tcagcagcct gatattaagc attcaactaa tacgctgctg actctgtccc
    40021 caacaccaga tattttccct aacccgatag tgtatgagaa aagatttaac gccgataaac
    40081 caatccttta tggttaggag aaaaaataat gtgattattc tgagccctta acattgatcg
    40141 ttatcaatta aaattacaa
//
LOCUS       DADMKF010000018        40077 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.018, whole genome shotgun sequence.
ACCESSION   DADMKF010000018 DADMKF010000000
VERSION     DADMKF010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40077
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.018"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(<1..675)
                     /locus_tag="JNC24_000884"
     CDS             complement(<1..675)
                     /locus_tag="JNC24_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001371776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9157705.1"
                     /translation="MSGKPAARQGDMTQYGGSIVQGSAGVRIGAPTGVACSVCPGGVT
                     SGHPVNPLLGAKVLPGETDIALPAPLPFILSRTYSSYRTKTPAPVGSLGPGWKMPADI
                     RLQLRDNTLILSDNGGRSLYFEHLFPGEDGYSRSESLWLVRGGVAKLDEGHRLAALWQ
                     ALPEELRLSPHRYLATNSPQGPWWLLGWCERVPEADEVLPAPLPPYRVLTGLVDRFGR
                     TQTFHRE"
     gene            complement(835..1047)
                     /gene="rpmE"
                     /locus_tag="JNC24_000885"
     CDS             complement(835..1047)
                     /gene="rpmE"
                     /locus_tag="JNC24_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HAY9157706.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            1250..3448
                     /gene="priA"
                     /locus_tag="JNC24_000886"
     CDS             1250..3448
                     /gene="priA"
                     /locus_tag="JNC24_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HAY9157707.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMAVKAGCRVRVPFGKQQERIG
                     VVVSVSDVSELPLNELKAVVEVLDVEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQTLRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPDVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIINGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            3604..4629
                     /gene="cytR"
                     /locus_tag="JNC24_000887"
     CDS             3604..4629
                     /gene="cytR"
                     /locus_tag="JNC24_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HAY9157708.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            4721..5680
                     /gene="ftsN"
                     /locus_tag="JNC24_000888"
     CDS             4721..5680
                     /gene="ftsN"
                     /locus_tag="JNC24_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HAY9157709.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            5773..6303
                     /gene="hslV"
                     /locus_tag="JNC24_000889"
     CDS             5773..6303
                     /gene="hslV"
                     /locus_tag="JNC24_000889"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HAY9157710.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            6313..7644
                     /gene="hslU"
                     /locus_tag="JNC24_000890"
     CDS             6313..7644
                     /gene="hslU"
                     /locus_tag="JNC24_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HAY9157711.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            7711..8637
                     /gene="menA"
                     /locus_tag="JNC24_000891"
     CDS             7711..8637
                     /gene="menA"
                     /locus_tag="JNC24_000891"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HAY9157712.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLVLITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQEMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            8730..9215
                     /gene="rraA"
                     /locus_tag="JNC24_000892"
     CDS             8730..9215
                     /gene="rraA"
                     /locus_tag="JNC24_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HAY9157713.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(9300..9545)
                     /gene="zapB"
                     /locus_tag="JNC24_000893"
     CDS             complement(9300..9545)
                     /gene="zapB"
                     /locus_tag="JNC24_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HAY9157714.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            9971..10816
                     /gene="glpF"
                     /locus_tag="JNC24_000894"
     CDS             9971..10816
                     /gene="glpF"
                     /locus_tag="JNC24_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
                     /protein_id="HAY9157715.1"
                     /translation="MSQTSTLKGQCIAEFLGTGLLIFFGVGCVAALKVAGASFGQWEI
                     SVIWGLGVAMAIYLTAGVSGAHLNPAVTIALWLFACFDKRKVIPFIVSQVAGAFCAAA
                     LVYGLYYNLFFDFEQTHHIVRGSVESVDLAGTFSTYPNPHINFVQAFAVEMVITAILM
                     GLILALTDDGNGVPRGPLAPLLIGLLIAVIGASMGPLTGFAMNPARDFGPKVFAWLAG
                     WGNVAFTGGRDIPYFLVPLFGPIVGAIVGAFAYRKLIGRHLPCDICVVEEKETTTPSE
                     QKASL"
     gene            10839..12350
                     /gene="glpK"
                     /locus_tag="JNC24_000895"
     CDS             10839..12350
                     /gene="glpK"
                     /locus_tag="JNC24_000895"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HAY9157716.1"
                     /translation="MTEKKYIVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPG
                     WVEHDPMEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAI
                     VWQCRRTAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGE
                     LLFGTVDTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEV
                     RRSSEVYGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNT
                     GEKAVKSENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFA
                     TKVQNTNGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDV
                     LEAMQADSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGL
                     AVGFWQNLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDVM"
     gene            12480..13490
                     /gene="glpX"
                     /locus_tag="JNC24_000896"
     CDS             12480..13490
                     /gene="glpX"
                     /locus_tag="JNC24_000896"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HAY9157717.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            13587..14333
                     /gene="fpr"
                     /locus_tag="JNC24_000897"
     CDS             13587..14333
                     /gene="fpr"
                     /locus_tag="JNC24_000897"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HAY9157718.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLEE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(14338..14766)
                     /gene="uspD"
                     /locus_tag="JNC24_000898"
     CDS             complement(14338..14766)
                     /gene="uspD"
                     /locus_tag="JNC24_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HAY9157719.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(14793..15092)
                     /locus_tag="JNC24_000899"
     CDS             complement(14793..15092)
                     /locus_tag="JNC24_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HAY9157720.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(15304..15744)
                     /locus_tag="JNC24_000900"
     CDS             complement(15304..15744)
                     /locus_tag="JNC24_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HAY9157721.1"
                     /translation="MTIQQWLFSFKGRIGRRDFWIWIGLWFAGMLVLFSLAGKNLLDI
                     QTAAFCLVCLLWPTAAVTVKRLHDRGRSGAWAFLMIVAWMLLAGNWAILPGVWQWAVG
                     RFVPTLILVMMLIDLGAFVGTQGENKYGKDIQDVKYKADNKSSN"
     gene            15845..16444
                     /locus_tag="JNC24_000901"
     CDS             15845..16444
                     /locus_tag="JNC24_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HAY9157722.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNSEKGLTFAVEPIKLALSESLEGLNK"
     gene            16552..17319
                     /gene="tpiA"
                     /locus_tag="JNC24_000902"
     CDS             16552..17319
                     /gene="tpiA"
                     /locus_tag="JNC24_000902"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HAY9157723.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(17374..18129)
                     /gene="cdh"
                     /locus_tag="JNC24_000903"
     CDS             complement(17374..18129)
                     /gene="cdh"
                     /locus_tag="JNC24_000903"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HAY9157724.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDNNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHQCEILR"
     gene            complement(18236..19225)
                     /locus_tag="JNC24_000904"
     CDS             complement(18236..19225)
                     /locus_tag="JNC24_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY9157725.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(19545..20507)
                     /gene="pfkA"
                     /locus_tag="JNC24_000905"
     CDS             complement(19545..20507)
                     /gene="pfkA"
                     /locus_tag="JNC24_000905"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HAY9157726.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(20688..21590)
                     /gene="fieF"
                     /locus_tag="JNC24_000906"
     CDS             complement(20688..21590)
                     /gene="fieF"
                     /locus_tag="JNC24_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709720.2"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HAY9157727.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLVSPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(21738..22238)
                     /gene="cpxP"
                     /locus_tag="JNC24_000907"
     CDS             complement(21738..22238)
                     /gene="cpxP"
                     /locus_tag="JNC24_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HAY9157728.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            22388..23086
                     /gene="cpxR"
                     /locus_tag="JNC24_000908"
     CDS             22388..23086
                     /gene="cpxR"
                     /locus_tag="JNC24_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HAY9157729.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            23083..24456
                     /gene="cpxA"
                     /locus_tag="JNC24_000909"
     CDS             23083..24456
                     /gene="cpxA"
                     /locus_tag="JNC24_000909"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HAY9157730.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(24562..25236)
                     /gene="yiiM"
                     /locus_tag="JNC24_000910"
     CDS             complement(24562..25236)
                     /gene="yiiM"
                     /locus_tag="JNC24_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein"
                     /protein_id="HAY9157731.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWVREFPEQAELFVAPAFGENLSTDGLTESNVHMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
     gene            complement(25385..26368)
                     /gene="kdgT"
                     /locus_tag="JNC24_000911"
     CDS             complement(25385..26368)
                     /gene="kdgT"
                     /locus_tag="JNC24_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HAY9157732.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(26628..27248)
                     /gene="sodA"
                     /locus_tag="JNC24_000912"
     CDS             complement(26628..27248)
                     /gene="sodA"
                     /locus_tag="JNC24_000912"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HAY9157733.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            27533..28567
                     /gene="rhaT"
                     /locus_tag="JNC24_000913"
     CDS             27533..28567
                     /gene="rhaT"
                     /locus_tag="JNC24_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HAY9157734.1"
                     /translation="MSNAITMGIFWHLIGAASAACFYAPFKKVKKWSWETMWSVGGIV
                     SWIILPWTISALLLPDFWAYYSSFSLSTLLPVFLFGAMWGIGNINYGLTMRYLGMSMG
                     IGIAIGITLIVGTLMTPIINGNFDVLINTEGGRMTLLGVLVALIGVGIVTRAGQLKER
                     KMGIKAEEFNLKKGLVLAVMCGIFSAGMSFAMNAAKPMHEAAAALGVDPLYVALPSYV
                     VIMGGGAIINLGFCFIRLAKVKDLSLKADFSLAKPLIIHNVLLSALGGLMWYLQFFFY
                     AWGHARIPAQYDYISWMLHMSFYVLCGGIVGLVLKEWNNTGRRPVTVLSLGCVVIIVA
                     ANIVGIGMAN"
     gene            complement(28564..29502)
                     /gene="rhaR"
                     /locus_tag="JNC24_000914"
                     /pseudo
     CDS             complement(28564..29502)
                     /gene="rhaR"
                     /locus_tag="JNC24_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709710.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
     gene            complement(29486..30322)
                     /gene="rhaS"
                     /locus_tag="JNC24_000915"
     CDS             complement(29486..30322)
                     /gene="rhaS"
                     /locus_tag="JNC24_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HAY9157735.1"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRAHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            30610..32079
                     /gene="rhaB"
                     /locus_tag="JNC24_000916"
     CDS             30610..32079
                     /gene="rhaB"
                     /locus_tag="JNC24_000916"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HAY9157736.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWNVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRSNGLMAQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPEAMCSEIQAACREMAQPLPESDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIQLMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQLHSTRQTKELCA"
     gene            32076..33335
                     /gene="rhaA"
                     /locus_tag="JNC24_000917"
     CDS             32076..33335
                     /gene="rhaA"
                     /locus_tag="JNC24_000917"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HAY9157737.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQAIGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHPDDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLDP
                     AHHIDAVESKLFGIGAESYTVGSNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPWQAVWEMYC
                     QRHDTPAGSEWLESVRAYEKAILSQRG"
     gene            33577..34401
                     /gene="rhaD"
                     /locus_tag="JNC24_000918"
     CDS             33577..34401
                     /gene="rhaD"
                     /locus_tag="JNC24_000918"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HAY9157738.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAP
                     YHGNFHAQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGVVKVDSDGAGY
                     HILWGLTNEAVPTSELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLVVFPDGVGILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSG
                     STLDETFGLIDTAEKSAQVLVKVYSMGGMKQTISREELIALGKRFGVTPLASALAL"
     gene            34411..34725
                     /gene="rhaM"
                     /locus_tag="JNC24_000919"
     CDS             34411..34725
                     /gene="rhaM"
                     /locus_tag="JNC24_000919"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HAY9157739.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGAHNYAIY
                     LDKARNLLFATVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
     gene            35026..35472
                     /locus_tag="JNC24_000920"
     CDS             35026..35472
                     /locus_tag="JNC24_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAY9157740.1"
                     /translation="MAALTASCIDLNIQGNGAYSVLKQLATIALQNGFITDSHQFLQT
                     LLLREKMHSTGFGSGVAVPHGKSACVKQPFVLFARKAQAIDWKASDGEDVNCWICLGV
                     PQSGEEDQVKIIGTLCRKIIHQDFIHQLQQGDADQVLALLNQTLSS"
     gene            35483..36934
                     /locus_tag="JNC24_000921"
     CDS             35483..36934
                     /locus_tag="JNC24_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIBC"
                     /protein_id="HAY9157741.1"
                     /translation="MESSLCIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQG
                     SSGVENRLSSEEIAAADYVILATGRGLSGDDRARFAGKKVYEIAISQALKNIDQIFSE
                     LPTNSQLFAADSGVKLGKQEVQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
                     LPYTDMSKGAPSFTWVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLAND
                     KALLGTQSGAGFLGAVVLGLAIGYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVL
                     TYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAAFLVGAMLAFDMGGPINKTAWFFCFSL
                     LEKHIYDWYAIVGVVALMPPVAAGLATFIAPKLFTRQEKEAASSAIVVGATVATEPAI
                     PYALAAPLPMITANTLAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLA
                     IGLALNISFIIVLKGLWLRRKAKAAQQELVHEH"
     gene            36924..37994
                     /locus_tag="JNC24_000922"
     CDS             36924..37994
                     /locus_tag="JNC24_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAY9157742.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGS
                     FVARKGNKGPKVAVVRHMDEVGFMVTHIDESGFLRFTTIGGWWNQSMLNHRVTIRTHK
                     GFKIPGVIGSVAPHALTEKQKQQPLSFDEMFIDIGANSRDEVEKRGVAMGDFISPEAN
                     FACWGEDKVVGKALDNRIGCAMMAELLQTVNNPEITLYGVGSVEEEVGLRGAQTSAEH
                     IKPDVVIVLDTAVAGDVPGIDNIKYPLKLGQGPGLMLFDKRYFPNQKLVAALKSCAAH
                     NDLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSGMISKADYDALLTLI
                     RDFLTTLTAEKVNAFSQFRQVD"
     gene            37994..39742
                     /locus_tag="JNC24_000923"
     CDS             37994..39742
                     /locus_tag="JNC24_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAY9157743.1"
                     /translation="MLNERQLKIVDLLEQQPRTPGELAQQTGVSGRTILRDIDYLNFT
                     LNGKARIFASGSAGYQLEIFERRSFFQLLQKHDNDDRLLALLLLNTFTPRAQLASALN
                     LPETWVAERLPRLKQRYERTCCLASRPGLGHFIDETEEKRVILLANLLRKDPFLIPLA
                     GVTRDNFQHLSTACDNQHRWPLMQGDYLSSLILAIYALRNQLTDEWPQYPGNEIKQIV
                     EQSGMFLGDNAVRTLTGLIEKQHQQAQVISADHVLGLLQRVPGIASLNIIDTQLVENI
                     TGHLLRCLAAPVWIAEHRQSSMNNLKAAWPAAFDMSLHFITLLREQLDIPLFDSDLIG
                     LYFACALERHQNERQPIILLSDQNAIATINQLAIERDVLHCRVIIARSLSELIAIREE
                     IEPLLIINNSHYLLDDAVNNYITVKNIITAADIEQIKHFLATAFIRQQPERFFSAPGS
                     FHYSNVRGESWQHITRQICAQLVAQHHITADEAQRIIAREGEGENLIVNRLAIPHCWS
                     EQERRFRGFFITLAQPVEVNNEVINHVLIACAAADARHELKIFSYLASVLCQHPAEVI
                     AGLTGYEAFMELLHKG"
     gene            complement(39791..>40077)
                     /locus_tag="JNC24_000924"
     CDS             complement(39791..>40077)
                     /locus_tag="JNC24_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YiiG family protein"
                     /protein_id="HAY9157744.1"
                     /translation="DVDAAMKKVSELETLVAQAKEADKGGMNFSFINSADQYQLETKK
                     YVRRVRDKVPYSDWDKEHLQDANTSWMVEDSFPRALREYNEMVDDYNSLR"
BASE COUNT         9704 a        10447 c        10437 g         9489 t
ORIGIN      
        1 ttcgcggtgg aacgtctgtg tgcgcccgaa gcggtccacc agcccggtca gtacccggta
       61 cggcggcagc ggcgcaggca gcacctcatc cgcttccggc acccgctcac accagccgag
      121 cagccaccac ggcccctgcg gactgtttgt cgccagataa cgatgcggac ttaagcggag
      181 ttcttccggc agcgcctgcc agagtgcggc cagccggtga ccttcatcca gtttcgccac
      241 gccgccgcgc accagccaga gtgattcgct gcggctgtaa ccgtcctcac cgggaaacag
      301 gtgctcaaaa tacaggcttc tgccgccgtt atcactgagt atcagtgtgt tatcgcgcag
      361 ctgtaagcgg atatccgcag gcattttcca gccggggccg aggctcccca ccggcgcggg
      421 cgtttttgtc cggtaactgc tgtaggtgcg ggagaggatg aacggcagcg gggcgggcag
      481 ggcgatgtcg gtttcaccgg gaaggacctt cgcaccgagc agcggattga ccggatggcc
      541 ggacgtcacc ccgccggggc acaccgaaca ggccacgccg gtgggggcac cgatgcgcac
      601 cccggctgaa ccctggacaa tgctaccgcc atactgcgtc atgtcgccct gacgcgccgc
      661 cggttttccg ctcatcgtaa aatccttttg aaataaataa gaaccagaag cctagcgcca
      721 gtcaacaaca ggaaatgata atgagttaac accccggaat ttttcagaat ttataaaagg
      781 caaattacag gcacaaaaaa agcgccgtgc ggcgcttttt tcggaaatcc ggtcttattt
      841 gctgcccggg atgttgaaac gcttgttgaa gcggtcaaca cggccaccgg tagcaacatc
      901 acgctgtttg ccagtgaaga acgggtggca cttgctgcac acgtcgaggt tcaggtcatg
      961 accaacggtg gagcggattt tcattacgtt accgcaagag cagctagcag taatttcttc
     1021 gtatttcggg tgaatatctt ttttcatggg aaaacctcgg tttaaggccg cgtcgctctt
     1081 ccagccctaa cgccagacac cacgcgatgt taaaagtata gcttcaatac gatcatttcg
     1141 tacgaaggcg cgaaatcata cagaaattaa ccagcgtatg caaactgatc cgcactcttc
     1201 tacggcaatg tgtatactaa cccaccgaat ttcaagtcag gatgatgcta tgcccgttgc
     1261 ccacgttgcc ttgcccgttc cgcttcctcg tacctttgac tatctgctgc cagaaggcat
     1321 ggcggttaaa gccgggtgtc gcgtgcgcgt gccgtttggc aaacagcagg agcgcatcgg
     1381 ggttgtggta tcagttagcg atgtcagtga gctgccgctt aacgagctga aagcggtagt
     1441 tgaagtgctg gacgttgagc cggtatttac tcactccgtc tggcgattac tgctctgggc
     1501 ggcagattac tatcatcatc cgattggcga tgtgctgttt catgccttgc cgattttact
     1561 gcgccagggg cggcctgcgg cgaacgcgcc gatgtggtac tggtttgcca ctgaacaagg
     1621 ccaggcggtg gatctgaaca gcctgaaacg ctcacccaag caacaacagg cgctggcggc
     1681 gttacggcaa gggaaaatct ggcgcgacca ggttgccacg ctcgaattta atgatgccgc
     1741 gttgcagacg ctgcgcaaaa aaggtctgtg tgatttagca agtgaaacac cagagtttag
     1801 cgactggcga acgaactatg ccgtttctgg tgagcggttg cgattgaata ccgaacaggc
     1861 caccgccgtt ggcgcaattc atagcgcggc agatactttt tctgcctggc tgctggcggg
     1921 cgttaccggt tccggtaaaa cggaggttta tctcagtgta ctggaaaacg tgctcgctca
     1981 gggcaaacag gcgctggtga tggtgccgga aatcggcctg acaccgcaaa ctatcgcccg
     2041 ttttcgtgaa cggtttaatg cgcccgtgga agttctgcat tccggtctga acgacagcga
     2101 gcgtctttcg gcgtggctga aagcgaaaaa tggcgaggcg gcgattgtga tcggcacccg
     2161 ctccgcgctg tttacgccgt ttaaaaatct cggcgtgatt gtcattgacg aagagcacga
     2221 cagttcctac aagcagcagg aaggctggcg ctatcatgcc cgcgacctgg cggtgtatcg
     2281 tgcgcatagc gagcaaatcc cgattattct tggctccgca acgcccgcgc tggaaacgtt
     2341 gtgcaacgtc cagcagaaaa aataccgcct gctgcgcctg acgcgccggg cggggaatgc
     2401 gcgtccggca attcaacacg tgctggattt aaaaggtcag aaggtgcagg caggtctggc
     2461 tccggcgtta atcacccgta tgcgccagca tttacaggcc aacaatcagg ttatcctctt
     2521 tcttaaccgc cgtggctttg cgcctgcgct actgtgccac gactgcggct ggattgccga
     2581 atgcccacgt tgcgatcact actacacgct gcatcaggcg caacagcatt tgcgctgtca
     2641 ccactgtgac agtcagcgtc cggtgccgcg ccagtgccct tcctgcggtt ccacgcacct
     2701 ggtacccgtg gggctgggca ccgaacagct tgaacagacg cttgcgccgc tgttccctga
     2761 tgtgcccatt tctcgtatcg accgcgatac caccagccgc aaaggggcgc tggaacagca
     2821 actggcggaa gtacatcgcg gcggtgcgag gattttgatc ggcacacaaa tgctggcgaa
     2881 aggtcaccac tttccggatg tgacgctggt ggcgttactg gatgtggacg gcgcgctgtt
     2941 ttctgccgat tttcgctcgg cagagcgttt cgctcagctt tacacccagg tcgccggtcg
     3001 tgccgggcgt gcgggtaaac agggcgaagt ggtgctgcaa acgcaccatc cggaacatcc
     3061 tctgttgcaa acgttgctct ataaaggcta cgacgccttt gccgaacagg cgctggctga
     3121 gcgacgcatg atgcagctgc cgccgtggac cagccatgtg attgtgcgtg cggaagatca
     3181 taacaatcag catgcgccgc tgttcctgca acaactgcgt aatctgatcc tctccagccc
     3241 gctggcagac gacaaattgt gggttctcgg tccggttccg gcactggcac ctaaacgtgg
     3301 cggtcgctgg cgctggcaga tattgttgca gcacccttcc cgcgtgcgct tgcaacacat
     3361 cattaacggt acgctggcac tcatcaatac aataccggat tcccgtaagg tgaaatgggt
     3421 gctggatgtt gatccgattg agggttaaac cgctcacgat gcgaggcgga tcgaaaaatt
     3481 caatattcat cacacttttc atgaaaattc tgtaaccgtt ttcacgcgct atctgctaaa
     3541 aatgttgccg atgtgaagta aacatggatg tagtacgcct gacgtgccag gcgaggagtg
     3601 agtgtgaaag cgaagaagca ggaaactgcc gcgaccatga aagacgttgc cctcaaggca
     3661 aaagtctcta cagcgactgt ctcccgagca ttaatgaatc ccgataaagt ctcccaggcc
     3721 acccgtaatc gggttgaaaa agcggcccgg gaagtgggtt atttaccgca gcctatgggg
     3781 cgcaacgtca agcgtaatga atcccgcacc attctggtga ttgtcccgga tatctgcgat
     3841 cccttcttta gcgaaattat tcgcggtatc gaagttacgg cggcaaatca cggatatctg
     3901 gtgctgattg gcgactgtgc gcatcaaaat cagcaggaaa aaacctttat cgatttgatc
     3961 atcaccaagc aaattgatgg catgttgctg ctgggttcaa ggctgccgtt tgatgccagc
     4021 attgaggaac agcgtaatct gccgccgatg gtgatggcga acgaattcgc accggaactg
     4081 gagctaccta cagttcatat cgacaatctg accgccgcat ttgatgcagt aaattatcta
     4141 tatgagcaag ggcataaacg gattggctgt atagccggtc ccgaagagat gccgctgtgt
     4201 cactaccgcc tgcaaggtta cgttcaggcg ctgcgtcgct gcggcattat ggttgatccg
     4261 caatacatcg cccgtggcga cttcaccttc gaagccggaa gcaaagcgat gcagcagctg
     4321 cttgatcttc cacaaccgcc tactgctgtc ttctgccata gcgatgtgat ggcgctcggc
     4381 gcactttctc aggcaaaacg ccaggggctg aaagtcccgg aagacctttc cataatcggt
     4441 tttgataaca tcgacctgac gcaattttgt gatccgccgc tgacaaccat cgcgcagccg
     4501 cgttacgaaa tcggtcggga agctatgctg ttattgcttg atcaaatgca ggggcaacac
     4561 gttggcagtg gctctcgttt aatggactgc gaacttatca tccggggatc aacacgcgcg
     4621 ttaccttaaa gtaaaccttt aagactgccg tgactggtca aaggcccgcc gcttaagtaa
     4681 catggcgggc tgacgaacga ataaatacag cgaaacgata gtggcacaac gagattatgt
     4741 acgccgcagc caaccggcac cttcgcggcg aaaaaagagc acctcacgga aaaagcaacg
     4801 aaatctgcct gcggtttctc ccgctatggt cgctattgct gccgccgtgc ttgtgacctt
     4861 tatcggtggt ctgtacttca ttacgcatca caagaaagaa gagtccgaga cgctgcaaag
     4921 ccagaaagtg accggtaacg gactaccacc aaaaccagaa gaacgctggc gctacattaa
     4981 agagctggaa agtcgccagc cgggtgtgcg tgcgcccaca gaaccttctg ccggtggtga
     5041 agtgaaaacg ccggagcaac tgacaccaga acaacgtcag cttcttgaac aaatgcaggc
     5101 tgatatgcgc cagcagccaa cgcagctggt tgaagttccg tggaatgaac agacgccgga
     5161 acagcgtcag caaacgctac aacgccagcg tcaggcgcag caattagcgg agcagcaacg
     5221 cctggctcag cagtcgcgta cgactgaaca aagctggcag cagcagacgc gtacgtcgca
     5281 agccgcgccg gtacaggcgc aaccgcgcca gtctaaaccg gcttctactc agcagccgta
     5341 ccaggatctg ctgcaaaccc ctgcgcacac gaccgcgcag tcgaagccgc agcaagctgc
     5401 gccggttgct cgtgccgctg acgcgccaaa accgacggcg gagaaaaaag acgaacgccg
     5461 ctggatggtg cagtgcggtt cgttcagagg cgcggaacag gcagagacgg tacgtgctca
     5521 gctggcgttc gaaggatttg actcgaaaat caccaccaac aatggctgga atcgtgtggt
     5581 cattggcccg gtgaaaggca aagagaacgc agacagcacc ctcaatcggt tgaagatggc
     5641 gggtcataca aactgcattc ggctcgccgc cgggggttga aaccctcaaa atccccccca
     5701 tctataattg cattatgccc cgtacttttg tacggggttt gtactctgta ttcgtaacca
     5761 aggggtcagc tcgtgacaac tatagtaagc gtacgccgta acggccatgt ggtcatcgct
     5821 ggtgatggtc aggccacgtt gggcaatacc gtaatgaaag gcaacgtgaa aaaggtccgc
     5881 cgtctgtaca acgacaaagt catcgcgggc tttgcgggcg gtactgcgga tgcttttacg
     5941 ctgttcgaac tgtttgaacg taaactggaa atgcatcagg gccatctggt caaagccgcc
     6001 gttgagctgg caaaagactg gcgtaccgat cgcatgctgc gcaaacttga agcactgctg
     6061 gcagtcgcgg atgaaactgc atcgcttatc atcaccggta acggtgacgt ggtgcagcca
     6121 gaaaacgatc ttattgctat cggctccggc ggcccttacg cccaggctgc ggcgcgcgcg
     6181 ctgttagaaa acactgaact tagcgcccgt gaaattgctg aaaaggcgtt ggatattgca
     6241 ggcgacattt gcatctatac caaccatttc cacaccatcg aagaattaag ctacaaagcg
     6301 taaggatctc ccatgtctga aatgacccca cgcgaaatcg tcagcgaact ggataagcac
     6361 atcatcggcc aggacaacgc caagcgttct gtggcgattg ctctgcgtaa ccgctggcgt
     6421 cgcatgcagc tcaacgaaga gctgcgccat gaagtgaccc cgaaaaatat cctgatgatc
     6481 ggcccgaccg gtgtcggtaa aactgaaatc gcccgtcgtc tggctaagct ggcgaatgcg
     6541 ccgttcatca aagttgaagc gaccaaattc accgaagtgg gctatgtcgg taaggaagtg
     6601 gattctatta ttcgcgatct gaccgatgcc gccgtgaaaa tggtacgcgt ccaggctatc
     6661 gagaaaaacc gttatcgcgc tgaagaactg gcagaagaac gtattctcga cgtgctgatc
     6721 ccacctgcta aaaacaactg gggacagacc gaacagcagc aggaaccgtc cgctgctcgt
     6781 caggcattcc gcaaaaaact gcgtgaaggc cagcttgatg acaaagaaat cgagatcgat
     6841 cttgccgcag caccgatggg cgttgaaatt atggctcctc caggcatgga agagatgacc
     6901 agccagctgc agtccatgtt ccagaacctg ggcggccaga agcaaaaagc gcgtaagctg
     6961 aaaatcaaag acgccatgaa gctgctgatt gaagaagaag cggcgaaact ggtgaacccg
     7021 gaagagctga agcaagacgc tatcgacgct gttgagcagc acgggatcgt gtttatcgac
     7081 gaaatcgaca aaatctgtaa gcgcggcgag tcttccggtc cggatgtttc tcgtgaaggc
     7141 gttcagcgtg acctgctgcc gctggtagaa ggttgcaccg tttccaccaa acacgggatg
     7201 gtcaaaactg accacattct gtttatcgct tctggcgcgt tccagattgc gaaaccgtct
     7261 gacctgatcc cggaactgca aggtcggctg ccaatccgcg ttgaactgca ggcactgacc
     7321 accagcgact tcgagcgtat tctgaccgag ccgaatgcct ctatcaccgt gcagtacaaa
     7381 gcactgatgg cgactgaagg cgtaaatatc gagtttaccg actccggtat taagcgcatc
     7441 gcggaagcgg catggcaggt gaacgaatct accgaaaaca tcggtgctcg tcgtttacac
     7501 actgttctgg agcgtttaat ggaagagatt tcctacgacg ccagcgattt aagcggtcaa
     7561 aatatcacta ttgacgcaga ttatgtgagc aaacatctgg atgcgttggt ggcagatgaa
     7621 gatctgagcc gttttatcct ataatcgcgt tcaatcattt tcatcattgt ttgatggggc
     7681 tgaaaggccc catttttatt ggcgcgtatt atgactgaac aacaaattag ccgaactcag
     7741 gcgtggctgg aaagtttacg acctaaaacc ctccccctcg cctttgctgc aattatcgtc
     7801 gggacggcgc tggcatggtg gcaaggtcac ttcgatccgc tggtcgccct gctggtacta
     7861 attaccgccg ggctattaca gatcctttct aacctcgcca atgattacgg cgatgcggta
     7921 aaaggcagcg ataaacctga ccgcattggg ccgctacgcg gcatgcaaaa aggggtcatt
     7981 acccagcaag agatgaaacg ggcgctcatt attaccgtcg tgctcatctg tctctccggg
     8041 ctggcactgg ttgcagtggc atgccatacg ctggccgatt ttgtcggttt cctgattctt
     8101 ggcgggttgt cgatcattgc cgctatcacc tacaccgtgg gcaatcgtcc ttatggttat
     8161 atcggtctgg gtgatatttc cgtactggtt ttctttggct ggttgagcgt catggggagc
     8221 tggtatttac aggctcatac attgattccg gcactgatcc ttccggcgac cgcctgtggc
     8281 ctgctggcaa cggcagtact gaacattaat aacctgcgtg atatcaatag cgaccgcgaa
     8341 aatggcaaaa acacgctggt ggtgcgctta ggtgaagtga acgcgcgtcg ttatcatgcc
     8401 tgcctgctga tgggctctct ggtatgtctg gcgctgttta atctcttttc gctgcatagc
     8461 ctgtggggct ggctgttcct gctggcggca ccattactgg tgaagcaagc ccgttatgtg
     8521 atgcgggaaa tggacccggt ggcgatgcga ccaatgctgg aacgtactgt caagggagcg
     8581 ttactgacta acctgctgtt tgttttaggg atattcctaa gccagtgggc agcataactg
     8641 acaaatatca attaacaatt gatgattttg ccaacagcct acatagcgcg atatactgaa
     8701 aattctcgca gcaactgaat gttaagccta tgaaatacga tacttccgag ctttgtgaca
     8761 tctatcaaga agatgttaac gtcgtggaac cgctgttctc caactttggc ggacgggcgt
     8821 cgtttggcgg acaaataatc acggtaaaat gtttcgagga caacgggttg ctgtacgatc
     8881 tgctcgaaca gaatggccgt ggtcgtgttc ttgtcgtcga tggcggtggt tctgttcgtc
     8941 gcgcactggt cgatgctgaa ctggcgcgtc tggcagtaca aaatgaatgg gaaggtctgg
     9001 ttatttacgg cgcggtgcgt caggtagatg acctagaaga gttggatatc ggcatccagg
     9061 cgatggcggc aattccggtt ggtgccgctg gcgaaggcat tggcgaaagc gatgtccgcg
     9121 tcaattttgg cggtgtaacc ttcttctccg gcgaccatct ttatgccgac aataccggga
     9181 ttattctttc agaagatccg ctggatattg aatgataaga aaggcaccgc aaggtgcctt
     9241 ttttctgcgt tacctgttgg cctacacagt aaagaaatta cgcgaaagat gaagcgtaat
     9301 cagacctctt ccatgcgacc cagcagggcc tgcagacgtt cctgccagcc gttctgctgt
     9361 tctttcagat ggttgttctc acgctccagc tcttcgcgct gatgctgggc attttgaact
     9421 tcctgcgaca gtgagttgtt tttttctttc agctcttcga tttccatctg caacagagtg
     9481 atggtatcaa tcgcctgctg tacttttgct tccagtttct caaacacttc taatgacatt
     9541 gtcatacctc tcctgaattg caaggcgttg atggataaaa aatcctcgtc ccgattaccg
     9601 gtgacgcctt aataaatacg agcgcacttt agttagctcc gattgtatga agccgcgcca
     9661 tcgctgtcca gcggcacgcc ttgcagatta cggtttgcca cacttttcat ccttctcctg
     9721 gtgacataat ccacatcaat cgaaaatgtt aataaatttg ttgcgcgaat gatctaacaa
     9781 acatgcatca tgtacaatca gatggaataa attgcgcgat aacgctcatt ttatgacgaa
     9841 gcacacacat tttaagttcg atatttctcg tttttgctcg ttaacgataa gtttacagca
     9901 tgcctacaag catcgtggag gtccgtgact ttcacgcata caacaaacat taactcttca
     9961 ggatccgatt atgagtcaaa catcaacctt gaaaggccag tgcattgctg aattcctcgg
    10021 taccgggttg ttgatttttt tcggtgtggg ttgcgttgca gcactaaaag tcgctggtgc
    10081 gtcttttggt cagtgggaaa tcagtgtcat ttggggactg ggggtggcaa tggccatcta
    10141 cctgaccgca ggggtttccg gcgcgcatct taatcccgct gttaccattg cattgtggct
    10201 gtttgcctgt ttcgacaagc gcaaagttat tccttttatc gtttcacaag ttgccggcgc
    10261 tttctgtgct gcggctttag tttacgggct ttactacaat ttatttttcg acttcgagca
    10321 gactcatcac attgttcgcg gcagtgttga aagtgttgat ctggcaggca ctttctctac
    10381 ttaccctaat cctcatatca attttgtgca ggctttcgca gttgagatgg tgattaccgc
    10441 tattctgatg gggctgatcc tggcgttaac ggacgatggc aacggtgtac cacgcggccc
    10501 tttggctccc ttgctgattg gtctactgat tgcggtcatt ggcgcatcta tgggcccatt
    10561 gacgggtttt gccatgaacc cagcgcgtga cttcggtccg aaagtctttg cctggctggc
    10621 gggctggggc aatgtcgcct ttaccggcgg cagagacatt ccttacttcc tggtgccgct
    10681 ttttggccct atcgttggcg cgattgtagg tgcatttgcc taccgcaaac tgattggtcg
    10741 ccatttgcct tgcgatatct gtgttgtgga agaaaaggag accacaactc cttcagaaca
    10801 aaaagcttcg ctgtaatatg actacgggac aattaaacat gactgaaaaa aaatatatcg
    10861 ttgcgctcga ccagggcacc accagctccc gcgcggtcgt aatggatcac gatgccaata
    10921 tcattagcgt gtcgcagcgc gaatttgagc aaatctaccc aaaaccaggc tgggtagaac
    10981 acgacccaat ggaaatctgg gccacccaaa gctccacgct ggtagaagtg ctggcgaaag
    11041 ccgatatcag ttccgatcaa attgcagcta tcggtattac gaaccagcgt gaaaccacta
    11101 ttgtctggga aaaagaaacc ggcaagccta tctataacgc cattgtctgg cagtgccgtc
    11161 gtaccgcaga aatctgcgag catttaaaac gtgacggttt agaagattat atccgcagca
    11221 ataccggtct ggtgattgac ccgtactttt ctggcaccaa agtgaagtgg atcctcgacc
    11281 atgtggaagg ctctcgcgag cgtgcgcgtc gtggtgaatt gctgtttggt acggttgata
    11341 cgtggcttat ctggaaaatg actcagggcc gtgtccatgt gaccgattac accaacgcct
    11401 ctcgtaccat gttgttcaac atccataccc tggactggga cgacaaaatg ctggaagtgc
    11461 tggatattcc gcgcgagatg ctgccagaag tgcgtcgttc ttccgaagtg tacggtcaga
    11521 ctaacattgg cggcaaaggc ggcacgcgta ttccaatctc cgggatcgcc ggtgaccagc
    11581 aggccgcgct gtttggtcag ttgtgcgtga aagaagggat ggcgaagaac acctatggca
    11641 ctggctgctt tatgctgatg aacaccggcg agaaagcggt gaaatcagaa aacggcctgc
    11701 tgaccaccat cgcctgcggc ccgactggcg aagtgaacta tgcgctggaa ggtgcggtgt
    11761 ttatggcagg cgcatccatt cagtggctgc gcgatgagat gaagttgatt aacgacgcct
    11821 acgattccga atatttcgcc accaaagtgc aaaacaccaa tggtgtgtat gtggttccgg
    11881 cctttaccgg gctgggtgcg ccgtactggg acccgtatgc gcgcggggcg attttcggtc
    11941 tgactcgtgg ggtgaacgct aaccacatta tacgcgcgac gctggagtct attgcttatc
    12001 agacgcgtga cgtgctggaa gcgatgcagg ccgactctgg tatccgtctg cacgccctgc
    12061 gcgtggatgg tggcgcagta gcaaacaatt tcctgatgca gttccagtcc gatattctcg
    12121 gcacccgcgt tgagcgcccg gaagttcgcg aagtgactgc attgggcgcg gcctatcttg
    12181 ctggtctggc ggtcggtttc tggcagaacc tcgacgagct gcaagagaaa gcggtgattg
    12241 agcgcgagtt ccgtccaggc atcgaaacca ctgagcgtaa ttaccgttac gcaggctgga
    12301 aaaaagcggt taaacgcgcg atggcgtggg aagaacacga cgtaatgtaa atgccggatg
    12361 aagcgtttat gccgcatccg gtagtcccga aacgtgcggg ggcaaccccg cacacatcaa
    12421 taatccctcc cttcccctgt gctacacttc gcgccattcc ttactgctta gagtttgcta
    12481 tgagacgaga acttgccatc gaattttccc gcgtcaccga atctgcggcg ctggctggct
    12541 acaaatggtt aggacgcggc gataaaaaca ccgcggacgg cgcggcggta aacgccatgc
    12601 gtattatgct caaccaggtc aatattgacg gcaccatcgt cattggtgaa ggtgaaatcg
    12661 acgaagcacc gatgctctac attggtgaaa aagtcggtac tggtcgcggc gacgcggtag
    12721 atattgctgt tgatccgatt gaaggcacgc gcatgacggc gatgggccag gctaacgcgc
    12781 tggcggtgct ggcagtaggc gataaaggct gcttcctcaa tgcgccggat atgtatatgg
    12841 agaagctgat cgtcggaccg ggagccaaag gcaccattga tttgaacctg ccgctggcgg
    12901 ataacctgcg caatgttgcg gcggcgctcg gcaaaccgtt gagcgaactg acggtaacaa
    12961 ttctggctaa accacgccac gatgccgtta tcgctgaaat gcaacaactc ggcgtacgcg
    13021 tatttgctat tccggatggc gacgttgcgg cctcaattct cacctgtatg ccagacagcg
    13081 aagttgacgt gctgtacggt attggtggcg cgccggaagg cgtagtttct gcggcggtga
    13141 tccgcgcatt agatggcgac atgaacggtc gtctgctggc gcgtcatgac gtcaaaggcg
    13201 acaacgaaga gaatcgtcgc attggcgagc aggagctggc acgctgcaaa gcgatgggca
    13261 tcgaagccgg taaagtattg cgcctgggcg atatggcgcg cagcgataac gtcatcttct
    13321 ctgccaccgg tattaccaaa ggcgatctgc tggaaggcat tagccgcaaa ggcaatatcg
    13381 cgactaccga aacgctgctg atccgcggca agtcacgcac cattcgccgc attcagtcta
    13441 tccactatct ggatcgcaag gacccggaaa tgcaggtgca catcctctga ttgatttgat
    13501 cgattgagcc ttccagtcct tcgggactgg aatttttttg ttcggagaac gaagataagg
    13561 caagtcaatc aaaacaggag aaaaacatgg ctgattgggt aacaggcaaa gtcactaaag
    13621 tgcagaactg gaccgacgcc ctgtttagtc tcaccgttca cgcccccgtg cttccgttta
    13681 ccgccgggca attcaccaag cttggccttg aaatcgacgg cgaacgcgtc cagcgcgcct
    13741 actcctatgt taactcgccc gataatcccg atctggagtt ttacctggtc accgtccccg
    13801 atggcaaatt aagcccacga ctggcggcac tgaaaccagg cgatgaagtg caggtggtta
    13861 gcgaagcagc tggcttcttt gttctggagg aagtaccaga ttgcgaaacg ctatggatgc
    13921 tggcaaccgg tacagcgatt ggcccttatt tatcgattct gcaactaggc aaagatttag
    13981 atcgcttcaa aaatctggtc ctggttcacg ccgcacgtta tgccgccgac ttaagctatt
    14041 tgccactgat gcaagaactg gaaaaacgct acgaaggcaa actgcgcatt cagacggtgg
    14101 tcagtcggga aacggcagcg gggtcgctca ccggacggat accggcatta attgaaagtg
    14161 gggaactgga aagcgcgatt ggcctgccga tgaataaaga aaccagccat gtgatgctgt
    14221 gcggcaatcc acagatggtg cgcgatacac aacagttgct gaaagagacc cggcagatga
    14281 cgaaacattt acgtcgccga ccgggccata tgacagcgga gcattactgg taagcggtta
    14341 cttatcgata aacggcacga tgagcaaatc cgcactcatc ttattgatca tcccgcgata
    14401 tgccggcatc aaacggttga taaatgagtg gtgatgacca cagacaagga ggtcgcactg
    14461 ctctttttgc ataatttcca gcagtgtttc cggcatttct ccgcgttcaa tacgcagttt
    14521 tgtcttcggc cattgaatat ttttcgtcag tttatacagc ttgttatccg acttattctt
    14581 caacaattga agaatatctt ctgttgcagg gaagtagata cccggataca actcgcttaa
    14641 gccatcatca atatgaatta acgtcaggtg agcgtcatta tgtctggcga gctccagggc
    14701 tttattcacc agtaaggcat cttcttcatt cccggaaatt gccacgccaa tgtgtttata
    14761 agccatgttt aactccttct aaagcaactc catcaagcta acgaacgcag tgataactca
    14821 aaaataatca tctcggcctg gcatgagaaa gtgaaggccg catcaagctc aacttgcccc
    14881 tccagatcct taatcttata ggccacgctg gccccttcat ctgcagagga tgcagcggcg
    14941 gcaagctctt tcagtttggt cataaaagac tctgcttctt cgcgggtggc aaaaaagcgc
    15001 gaaaacttac tggtacagtt gtcgttatca atcaccgtac cgatatcaat cgcacatcct
    15061 ttagtactgc atttatctac gacatctttc atagggcacc tctgtatatt cgcctctctg
    15121 tcgattacga cttaagaata atgcgccgtt aagggaagaa caaacaagta atgatcttat
    15181 ggattaccat tttttttgat attgcttaag gtgaagagtt cagaaggatt ttccgggtat
    15241 tgtcatttgc atcgtggaaa taacaatccc ggaaaaaaat agcagtaaga gatatattaa
    15301 aggttaatta cttgatttat tgtcggcttt atacttcaca tcctgaatat ctttaccata
    15361 tttattttcg ccttgtgtgc caacaaacgc accaagatcg ataagcatca tcaccagaat
    15421 caacgtcggg acaaaacgcc ccaccgccca ttgccagaca cccggtaaaa tcgcccagtt
    15481 acccgccagc agcatccacg ccacaatcat cagaaatgcc catgcgccgg aacgcccgcg
    15541 atcatgcaag cgcttaacag ttactgccgc tgttggccaa agcaagcaca caaggcaaaa
    15601 cgccgcggtc tgaatatcga gtaaattttt acccgccagt gagaaaagca ccagcatgcc
    15661 tgcgaaccac aggcctatcc aaatccagaa atcacggcgt ccaatacgcc ctttaaatga
    15721 gaataaccat tgctgtatgg tcatgtaagt tccttgatgg ttgtcttttc caggattcta
    15781 cccttttgac aagggcgaca gatatcgttt taatcggagc cagtcataac aaaaggtact
    15841 gtcaatgaag ccagggtgta cgctgttttt tctcttatgt tctgcattaa ccgttacaac
    15901 aacggcgcat gcacaaacac cagatacggc aacgaccgcg ccttatctgc tggctggagc
    15961 ccctactttc gatctctcca tcagccagtt tcgagaagac tttaacagcc agaatcctag
    16021 cctgccactg aacgaatttc gtgccatcga cagcagtccc gacaaagcca atctcactcg
    16081 tgctgcaagt aaaattaacg agaacttgta tgcttctaca gcgctggagc gcggtacctt
    16141 aaaaatcaaa agcattcaaa tgacctggct acccatccag gggccagagc aaaaagcagc
    16201 gaaagcgaaa gctcaggaat acatggcagc agtgatccgc acactcaccc cattaatgac
    16261 caaaacacaa agccagaaaa aactgcagtc gctactaacg gcggggaaaa acaaacgtta
    16321 ttacaccgag acagaaggtg cactgcgtta tgttgtcgcg gacaacagcg aaaaggggct
    16381 gaccttcgct gttgaaccga ttaagctggc gctatctgaa tcgcttgaag gtttgaataa
    16441 atgacaaaaa gcaaagcctt tgtgccgatg aatctctata ctgtttcaca gacctgctgc
    16501 cctgcggggc ggccatcttc ctttattcgc ttataagcgt ggagaattaa aatgcgacat
    16561 cctttagtga tgggtaactg gaaactgaac ggcagccgcc acatggttca cgagctggtt
    16621 tctaacctgc gtaaagagct ggcaggtgtt gctggctgtg cggttgcaat cgcaccaccg
    16681 gaaatgtaca tcgatatggc gaagcgcgaa gctgaaggca gccacatcat gctgggtgcg
    16741 caaaacgtgg acctgaacct gtccggcgca ttcaccggtg aaacttctgc cgctatgctg
    16801 aaagacatcg gcgctcagta catcatcatc ggccactctg aacgtcgtac ttaccacaaa
    16861 gagtctgacg aactgatcgc gaaaaaattc gcggtgctga aagagcaggg cctgactccg
    16921 gttctgtgca tcggtgaaac cgaagctgaa aacgaagcgg gcaaaactga agaagtttgc
    16981 gcacgtcaga tcgacgcggt actgaaaact cagggtgctg cggcattcga aggtgcggtt
    17041 atcgcttacg aacctgtatg ggcaatcggt actggcaaat ctgcaactcc ggctcaggca
    17101 caggctgttc acaaattcat ccgtgaccac atcgctaaag ttgacgctaa catcgctgaa
    17161 caagtgatca ttcagtacgg cggctctgta aacgcgtcta acgctgcaga actgtttgct
    17221 cagccagata tcgacggcgc gctggttggc ggtgcttctc tgaaagctga cgctttcgca
    17281 gtgatcgtta aagctgcaga agcggctaaa caggcttaag tctgacaggt gccggatttc
    17341 atatccggca cttactttcc ttaactcttc gccttaacgc aaaatctcac actgatgatc
    17401 ctgaatttcc tcggctgaag cacggttaag cgtcagtaga tttcgttgtg tcgccagcaa
    17461 tacaaatgag ttatcactct gccgtaccat cgccagcccg tagcttccca tatgttcccg
    17521 cgcctcaggt acttcttctg ccagcatcat aaatgggctg cgttgtacca gttcgctttc
    17581 cgttacccga cgcgccaggt attcatgccc acgcaaacca cctggcagtg gcaaccagcg
    17641 gctgctgatg ttcgccagat tgttatccag ttgttcgcgc acatcaggac gaatacaaga
    17701 gatatgaata tgaaaatggt tttgcgtacg cccggtgcgg gagttgatcg ccaaagaaac
    17761 cgcgcgatcg ggaaccggct ggccgtattt tttgctcata aaatcacgcg cctgccaggc
    17821 caaccaaaag aagttcggcg ttgaaggatc ggtcaacaaa ggactttcag taccgttaat
    17881 acgatacgtt ggcatcaaca gatattgcag tggaccatta agatctttta aaaccacgta
    17941 tccggcattg ggtttgactt ccgcacatgg cgaaggattt tgattttgct gctgattggg
    18001 caaacattcc tcaaggacaa tcttacgtaa tgtatccgac tcttcaccgg ttaatttcca
    18061 gtaaccaata ccggcagcca caacggcgat aactatcatc accaaaaaaa gaagacccgc
    18121 ttttttcatc tttttttccc tgtacctcaa agagatgcaa gggtaacgca aaatcgtgac
    18181 aaataaaaaa cccggtcgcg agaaaccacc gggtttgata attatcctgg ggaaatcagc
    18241 gtttgctgat ctgatcgaac gtaccgccgt tagcaaaatg ctctttttgc gctttcgtcc
    18301 agccgccgaa ctcttcatca atggtgaata acttcagctt tggaaacgca ttttcgtact
    18361 ttttcgctac ttcagcgtcg cgcggacggt agtagttttt cgcggcaatt tcctgacctt
    18421 ctggcgagta gagatatttc aggtaggctt ccgccacctc tttggtgcct tttttctcga
    18481 ccactttatc gaccaccgac acggttggct ctgcgaggat agactcactc ggcgtgacga
    18541 tttcgaattt atctttcccc agttcattcg ctgccagcag agcttcgttt tcccaggcga
    18601 tcagtacatc gccaatcccg cgctcgacaa aggtgttagt ggagccgcgc gcgccagaat
    18661 ccagaacttc gacgttttta tacagtgccc gaacaaaatc ctgtgctttt gcctgatcgt
    18721 tgttgttgtg atgcagcgcg tagccccagg cagctaggta gttccagcgt gcgccaccag
    18781 agcttttcgg attaggcgtg atcaccgaaa cacccggttt aatcagatcg ttccagtcat
    18841 ggatctgctt cggattgccc ttacgcacca ggaaaacaat ggtggaagtg tacggtgcgg
    18901 agttatccgg cagacgtttg atccactctt tatcaatccg cccacgttcc gcaatcgcgt
    18961 ccacgtcata ggccagagcc agcgtgacaa catcagcttc aataccgttg attaccgacg
    19021 tcgcttgttt acctgaacca ccgtgtgact gacgaatcac cacgttatca ccagtttgct
    19081 gtttccagtg ggcgctgaat gccttgttgt actgttcgta caattcgcgc gttggatcat
    19141 atgaaacgtt aagaagctga atatcctttg ccataacgct ggttgccgcc agcaaaaatg
    19201 ttaaccctac gccccacttg ttcatcgccc gactctctta tgttgtgttg tgatgagcaa
    19261 agcgtgccag aaggttaacc aaacattaaa gaataaaaaa agattggcta taacttgcgg
    19321 gtatatgttg agggattaaa aaggcggaag aaatccgcct catattgctg acaaagtgcg
    19381 ctttgtccat gccggatgcg gcgcgaacgc cttatccggc ctacaaaagt ttgcaaattc
    19441 aataaattgc agaattcatg taggcctgat aagcgaagcg catcaggcat ttttgcttct
    19501 gtcatcggtt tcaggctaaa ggaatctgcc tttttccgaa atcattaata cagttttttc
    19561 gcgcagtcca gccagtcacc tttgaacgga cgcttcatgt tttcgatagc gtcgatgatg
    19621 tcgtggtgaa ccagctgttc gttctggata cctacacaac gaccgccgta acctgccagc
    19681 agcagatcga tagcgtaagc gcccatacgg gaagccagaa tacggtcgta aggcaccgga
    19741 gaaccaccgc gctggatgtg gcccagcaca gttgcgcggg tttcacgacc ggtttctttc
    19801 tcgatgaaat gcgccagttc gtcaacatca cacatatgtt cggtaatcgc cacgatcgcg
    19861 tgttttttac ctttcgcgat acccgctttg atttcgttta ccaggtcttc acggctgaat
    19921 tcaacttccg gaaccacaac gaattcacag ccaccggcaa tggccgcagc caacgtcaga
    19981 tcgccacaat aacggcccat cacttccacc acggaaatac gctggtgaga agaagaggtg
    20041 tcacgcagac ggtcgatcgc ttctacaacg gtgctcagcg cagtgaagaa accgatagtg
    20101 tagtcagtgc ctttgatgtc gttgtcgata gtgcccggca gaccgatgca cgggaagccc
    20161 atttcggtca gacgcattgc acccatgtag gaaccgtcac cgccgataac caccagcgcg
    20221 tcgataccac gttttttcag gttttcgata gccacggcgc ggatgttctc gtcgcggaat
    20281 tccgggaagc gcgcagaacc gaggaacgta ccgccacggt tgatcatgtc agaaacgctg
    20341 taacggtcta gctgtaccat acggtcttca tacagaccca gatagccgtc ataaatgccc
    20401 attacttcca gaccttctgt cagcgcagaa cgaacaaccc cgcgaattgc ggcgttcatg
    20461 cctggcgcat caccgccgct tgtcaacaca ccgattttct taatcatgac tacctctgaa
    20521 ctttggaatg caaaataaaa tctgttgccg gaagtcttct tgcacatcga agtgatccaa
    20581 cgaatgtgca aatagtataa caatcacttc ctgctgaatt gattcaggtc aggccaaatg
    20641 gcggtatttt atacacaaaa tgcgggtctg gctctctttt atactgatta tgaaagcata
    20701 gaccgtttac cctccctggg tacgacggaa caggggtcct gatggataat tacatccgat
    20761 cccggaaaac gccgtaaaat agcctgctct acctgatccg ccaccatatg tgcctgaacc
    20821 aaaggcagag agtcttccat ttccaaatga atctgaataa agcgggtcgg ccctgactgc
    20881 cgcgtgcgaa gatcgtgagc gccgctaaca cccggccagg aagtcacgat atcaataatt
    20941 tcttgccgtt cctcatcagg caatgcgcga tccagtaatg actgtaccgc ctcatatccc
    21001 atgcgtaacg cgctatataa aatatagatg ccgattccca atgcaaacag agcatcggcg
    21061 cgatgccagc cgtaccagga caaccccagc gccagcagaa ttgcgccgtt catcataaca
    21121 tcagactggt aatgtagcat atcagcccgc accgcctggc tttgcgtccg gcgcaccacc
    21181 caccgctgaa acgagacaag gataatcgta caaattagcg ccacaattgt cacgataacc
    21241 ccgacgcctg gatctgtcat cggtgttgga gataccagat gttgaatacc cgtcaaaaac
    21301 aggaatagtg ccgaaccgga gataaacata ctttgcgcca gcgccgcgag ggactcagct
    21361 ttaccgtgac caaacgagtg attatcgtcg gcaggttgca gggaatatcg caccaccagt
    21421 aaattcgtca acgacgcgcc gatatccacc agcgaatcca ccagcgcggc gagaatactc
    21481 accgacccgg tataccacca tgcaaaaatt ttaatcagca atagcagcga agccatcgcc
    21541 gtcgcagcaa tcgccgcccg actgaccagc cgtccataag attgattcat aaatactccc
    21601 actatcaact gacgctagta taacggaagc aaatcatctg caatgcatta agcagcaggc
    21661 aaattgagga taaaaaaaac cccacatcat gtgggggaag acagggatgg tgtctatggc
    21721 aaggaaaaca gggtttacta ctgggaacgt gagttgctac tactcaatag cttcaacgat
    21781 gaactttttt gccattgcgt cacgtcacgc aactgctcca ttcgttgttg atgtttctcg
    21841 tttaaaaccg cttgctgctc cggcgttaac aggcgataca tttggttgcg gacttttgcc
    21901 atctcaacct gacgagcaat ttgctcattc gccatttttt ctgcctgtgc gcgcacagcg
    21961 ttttcatcaa aattttctgc ggtgacaagg cgatgcattg tctccagttc gctaacatta
    22021 acaggaggct gttcgtgccg ggcctgttgc ataagatctc gcatctgctg acgctgatgt
    22081 tcggttaaac ttatgccgtc gaacatatgg ctctgcgtac tgcgctgcgt aagttcttca
    22141 cccggatgcc agttatcgcc tgaaccgact tcagcagcgt ggcttaatga actgactgcc
    22201 agcgttgagg ccatgacggc agcggtaact atgcgcatca tttgctccca aaatctttct
    22261 gtcgcgattc aacgatagag agtttacgat tcaggctgca aacatgcgtc agggggtgta
    22321 aaacaacgta aagtcatgga ttagcgacgt ctgatgacgt aatttctgcc tcggaggtat
    22381 ttaaacaatg aataaaatcc tgttagttga tgatgaccga gagctgactt ccctattaaa
    22441 ggagctgctc gagatggaag gcttcaacgt gattgttgcc cacgatgggg aacaggcgct
    22501 tgatcttctg gacgacagca ttgatttact tttgcttgac gtaatgatgc cgaagaaaaa
    22561 tggtatcgac acattaaaag cacttcgcca gacacaccag acgcctgtca ttatgttgac
    22621 ggcgcgcggc agtgaacttg atcgcgttct cggccttgag ctgggcgcag atgactatct
    22681 cccgaaaccg tttaatgatc gtgagctggt ggcacgtatt cgcgcgatcc tgcgccgttc
    22741 gcactggagc gagcaacagc aaaacaacga caacggttca ccgacactgg aagttgatgc
    22801 cttagtgctg aatccaggcc gtcaggaagc cagcttcgac gggcaaacgc tggagttaac
    22861 cggtactgag tttaccctgc tctatttgct ggcacagcat ctgggtcagg tggtttcccg
    22921 tgaacattta agccaggaag tgttgggcaa acgcctgacg cctttcgacc gcgctattga
    22981 tatgcacatt tccaacctgc gtcgtaaact gccggatcgt aaagatggtc acccgtggtt
    23041 taaaaccttg cgtggtcgcg gctatctgat ggtttctgct tcatgatagg cagcttaacc
    23101 gcgcgcatct tcgccatctt ctggctgacg ctggcgctgg tgttgatgtt ggttttgatg
    23161 ttacccaagc tcgattcacg ccagatgacc gagcttctgg atagcgaaca gcgtcagggg
    23221 ctgatgattg agcagcatgt cgaagcggag ctggcgaacg atccgcccaa cgatttaatg
    23281 tggtggcggc gtctgttccg ggcgattgat aagtgggcac cgccaggaca gcgtttgtta
    23341 ttggtgacca ccgaaggccg cgtgatcggc gctgaacgca gcgaaatgca gatcattcgt
    23401 aactttattg gtcaggccga taacgccgat catccgcaga agaaaaagta tggccgcgtg
    23461 gaactggtcg gtccgttctc cgtgcgtgat ggcgaagata attaccaact ttatctgatt
    23521 cgtccggcca gcagttctca atccgatttc attaacttac tgtttgaccg cccgctatta
    23581 ctgctgattg tcaccatgtt ggtcagtacg ccgctgctgt tgtggttggc ctggagtctg
    23641 gcaaaaccgg cgcgtaagct gaaaaacgct gccgatgaag ttgcccaggg aaacttacgc
    23701 cagcacccgg aactggaagc ggggccacag gaattccttg ccgcaggtgc cagttttaac
    23761 cagatggtca ccgcgctgga gcgcatgatg acctctcagc agcgtctgct ttctgatatc
    23821 tctcacgagc tgcgcacccc gctgacgcgt ctgcaactgg gtacggcgtt actgcgccgt
    23881 cgtagcggtg aaagcaagga actcgagcgt attgaaaccg aagcgcaacg tctggacagc
    23941 atgatcaacg atctgttggt gatgtcacgt aatcagcaaa aaaacgcgct ggttagcgaa
    24001 accatcaaag ccaaccagtt gtggagtgaa gtgctggata acgcggcgtt cgaagccgag
    24061 caaatgggca agtcgttgac agttaacttc ccgcctgggc cgtggccgct gtacggcaat
    24121 ccgaacgccc tggaaagtgc gctggaaaac attgttcgta atgctctgcg ttattcccat
    24181 acgaagattg aagtgggctt tgcggtagat aaagacggta tcaccattac ggtggacgac
    24241 gatggtcctg gcgttagccc ggaagatcgc gaacagattt tccgtccgtt ctatcggacc
    24301 gatgaagcac gcgatcgtga atctggcggt acaggattgg ggctggcgat tgttgaaacc
    24361 gccattcagc agcatcgtgg ctgggtgaag gcagaagaca gcccgctggg cggtttacgg
    24421 ctggtgattt ggttgccgct gtataagcgg agttaaactc cgcatttgta ggcaggataa
    24481 ggcgtttacg ccgcatccgg catttgagca ggatgcctga tgcgacgctg atagcgtctt
    24541 atcaggccta cactccgggg gttattttcc ccacaaccgg cgggaaaaat cttcgatctt
    24601 gccgctcagt cggcgctttt gcatcgtccg cgtccagctt ttcgataacc cggcggcgga
    24661 gagtaaacgg tgatactgat cgtcatcaaa cggcatatgc catgcgatgg cggcagcctc
    24721 ctgcacggtc acatcgctga cacgggaaac caactccagc ggcgcgtccg cagacacttt
    24781 ccctggcgct atcacgctat acaaccaccc caccttacct gtgttttgca tcaactgcgc
    24841 tatatcgctg atatcaaaat ggtaattgag tttatagcaa ggcgagcgcg gctggctgac
    24901 ctgaattaat gcctctcccc agcggaaaat atcgcccata tgaacattac tttccgtgag
    24961 accgtcggtt gagagatttt cgccaaacgc aggcgcaaca aacaactccg cctgttccgg
    25021 aaattcccgc acccagtaga gatagtgctc acgaggataa tgacacagcg ctctgtctgg
    25081 cccgccgtga acctttttct ccgcctgctc gtcaccttcc agccccagct ccgtcagcat
    25141 caactcacca tcaacctgga ttttggcaat tgcgctgggt ttgccttcgg gataagcctg
    25201 aatcttgcct gtgtatacat caaccggata tcgcatctgt tccctctcca cgagaaattt
    25261 ccccattaga ccatatttat tttatgtcag gcgtgctgtt ttcgcggtaa gtatccaggc
    25321 aggaactcaa tagcaaaccg gacgaacgtt agttcatccg gctgtgggga ttaaggaagc
    25381 aggtttattt caccgtacct aaaatttcga ttttcgctgc tctggctttg actttacgtg
    25441 accagataga ggtaagaatt ggcaccagaa tcgaagtcac aatgaccgcc gtcgctacca
    25501 gcgaagttgc tgccggagcc atcggtttaa acgcaggcac catttctgca atcagcacag
    25561 gtgtcgctac cgcggcccct gcggaactgg aagcggcaat tccggcagtg ccatcgccac
    25621 cgccaatcaa tttatcggcg ataatcaacg gaataccggt cacgataatt actgccacac
    25681 ccaacaggat ccccagcaaa ccagtctggg caatcacagt caaatcaatg gtattgccca
    25741 gcgcgaaggc aaagaatgga atcagcgttt gcactgcttt gctgaaaaat tctcgtaatt
    25801 cagggtcaag attccccagg gcaaagccca ccaggaacgg taatactgcg ccgacgaaaa
    25861 catgcggttc aaacgaggca atcccggcag tgcccagaat aatcatcgtc atgagcggcc
    25921 cggactccaa cgacatcaac acaaatgccc cagcttcttc tttcgtaccg tactgctgca
    25981 tgatggaagc gtaaagtccg ccgttggtca tatccatcgc cgccaccagc gccagcgttg
    26041 aaagtccggc aaagaatcca acttcaacac catgttccgg aataatgcgc gaggcaattg
    26101 ccgcaaccac ccacgcgacg gcaattttag ttaccaccag cgtaccggat ttacgcagta
    26161 ccgttcccgt cgcgcttaat tttattgacg cccccatgca aaaaaaccac accgccagaa
    26221 tgggcaccgt accggtaatc atcccgttgg tgaatgatcc aaaatatttc cccgcccccg
    26281 gcgagaaggt gtggcacagt gcgccaagga atagcgggac gagcatcatc ccccccggga
    26341 ttttctcaat cgagcgtttt atctgcattt ccatcacctg tcggcatatt tttaagggct
    26401 gatgcgtaat tcctcaatgg agcatcagtg ttgatggaaa cgaagataga cattcattta
    26461 atcaataaaa gtgatcaaaa ccacatcaat tgaaacgctg ttttattttt atcggatcat
    26521 tgtttttatt ttgaaattga tcacaaaaaa accaccgccg ttggcgatgg ttcatttttt
    26581 aagctgatat gcggcctata cgcctcattg cagcaggcgg caaatgatta ttttttcgcc
    26641 gcaaaacgtg ccgctgcttc gtcccagttc accacgttcc agaactcttt aatgtagtcc
    26701 ggacggcggt tctggaattt caggtagtaa gcatgttccc acacatccag gcccagaatc
    26761 gggaagccgg aagcgccaga aatagcttca cccatcagcg gagaatcctg gttagcagta
    26821 gaaaccaccg ccagtttatc gcctttcagc accagccatg cccagccgga accaaagcgg
    26881 gaagctgccg ctttttcaaa ttctgctttg aagttatcaa cggagccgaa gtcacgttcg
    26941 atagccgctt tcaggtcacc ctgcagggtg gtgccttttt tcagaccttt ccagaacagg
    27001 ctgtggttag cgtgaccgcc agcgttgttg cgcagtacgg ttttcttgtc tgctggcagc
    27061 tggtccagtt tggtgatcag ctcttcaacc ggcaggttgg caaattctgg caggctttcc
    27121 agcgccgcgt tggcgttgtt tacgtaggtc tgatggtgtt tggtgtggtg gatttccatg
    27181 gtctgcttat cgaagtgcgg ttccagggca tcgtaagcat acggcaggga tggcagggta
    27241 tagctcatat tcatctccag tattgtcggg cggccgattg ttaatgccgc gtaagcagtt
    27301 ggttcattat agttaattaa atgatattga aaatgattat caatgccgta cttttcgtaa
    27361 gggtatggtt ttgcaggaaa atgcccgaga tgtgaagcaa atcacccact taatgccgtg
    27421 attgccagta aatcgacaac ggcggcaaca ggcgaaaggt taatcgacag cacgattttt
    27481 acactcatct cgtcggagat gtgaagcgac gaaaaatgat gaggataaga agatgagtaa
    27541 cgcgattacg atggggatat tttggcattt gatcggcgcg gccagtgcag cctgttttta
    27601 cgctccgttc aaaaaagtaa aaaaatggtc atgggaaacc atgtggtcag tcggtgggat
    27661 tgtttcgtgg attattctgc cgtggaccat cagcgccctg ttactaccgg atttctgggc
    27721 gtattacagc tcgtttagtc tctctacgct gctgcctgtt tttctgttcg gcgctatgtg
    27781 ggggatcggt aatatcaact atggcctgac catgcgttat ctcggcatgt cgatgggaat
    27841 tggcatcgcc attggcatta cgttgattgt cggtacgctg atgacgccaa ttatcaacgg
    27901 caatttcgat gtgctgatta acaccgaagg cggacgcatg acgttgctcg gcgttctggt
    27961 ggcgctgatt ggcgtaggga ttgtaactcg agccgggcag ttgaaagagc gcaagatggg
    28021 cattaaagcc gaagagttca atctgaaaaa agggctggtg ctggcggtga tgtgcggcat
    28081 tttctctgcc gggatgtcct ttgcgatgaa cgccgcaaaa ccgatgcatg aagccgctgc
    28141 cgcacttggc gtcgatccac tgtatgtcgc tctgccaagc tatgttgtca tcatgggcgg
    28201 cggcgcgatc attaacctcg gtttctgttt tattcgtctg gcaaaagtga aggatttgtc
    28261 gctaaaagcc gacttctcgc tggcaaaacc gctgatcatt cacaatgtgt tactctcggc
    28321 actgggcggg ttgatgtggt atctgcaatt ctttttctat gcctggggcc acgcccgcat
    28381 tccggcgcag tatgactaca tcagttggat gctgcatatg agtttctatg tattgtgcgg
    28441 cggtatcgtc gggctggtgc tgaaagagtg gaacaataca ggacgccgtc cggtaacggt
    28501 gttgagcctc ggttgtgtgg tgattattgt cgccgctaac atcgtcggca tcggcatggc
    28561 gaattaatct ttctgcgaat tgagatgacg ccactggctg ggcgtcatcc cggtttcccg
    28621 ggtaaacacc accgaaaaat agttactatc ttcaaagcca cattcggtcg aaatatcact
    28681 gattaacagg cggctatgct ggagaagata ttgcgcatga cacactctga cctgtcgcag
    28741 atattgattg atggtcattc cagtctgctg gcgaaattgc tgacgcaaaa cgcgctcact
    28801 gcacgatgcc tcatcacaaa atttatccag cgcaaaggga cttttcaggc tagccgccaa
    28861 ccgggtaatc agcttatcca gcaacgtttc gctggatgtt ggcggcaacg aatcactggt
    28921 gtaacgatgg cgattcagca acatcaccaa ctgcccgaac agcaactcag ccatttcgtt
    28981 agcaaacgac acatgctgac tactttcatg ctcaagctga ccgataacct gccgcgcctg
    29041 cgccatcccc acgctaccta agcgccagtg tggttgccct gcgctggcgc taaatcccgg
    29101 aatcgctccc tgtcagtcaa gattcagctt cagccgctcc gggcaataaa taatattctg
    29161 caaaaccaga tcgttaacgg aagcgtagga gtgtttatcg tcagcatgaa tataaaagag
    29221 atcgccacgg gtaatgcgat aaggacgatc gttgagtaca tgcagaccat taccgcgcca
    29281 gacaatcacc agctcacaaa aatcatgtgt atgttcggca aagacatctt gcggataacg
    29341 gtcagccaca gcgactgcct gctggtcgct ggcaaaaaaa tcatctttga gaagttttaa
    29401 ctgatgcgcc accgtggcta cctcggccag agaacgaagt tgattattcg caatatggcg
    29461 tacaaatacg ttgagaagat tcgcgttatt gcagaaagcc atcccgtccc tggcgaatat
    29521 cacgcggtga ccagttaaac tctcggcgaa aaagcgtcga aaagtggtta ctgtcgctga
    29581 atccacagcg ataggcgatg tcagtaacgc tggcctcgct gtggcgtagc agatgtcggg
    29641 ctttcatcag tcgcaggcgg ttcaggtatc gctgaggcgt cagtcccgtt tgctgcttaa
    29701 gctgccgatg tagcgtacgc agtgaaagag aaaattgatc cgccacggca tcccaattca
    29761 cctcatcggc aaaatggtcc tccagccagg ccagaagcaa gttgagacgt gatgcgctgt
    29821 tttccaggtt ctcctgcaaa ctgcttttac gcagcaagag cagtaattgc ataaacaaga
    29881 tctcgcgact ggcggtcgag ggtaaatcat tttccccttc ctgctgttcc atctgtgcaa
    29941 ccagctgtcg cacctgctgc aatacgctgt ggttaacgcg ccagtgagac ggatactgcc
    30001 catccagctc ttgtggcagc aactgattca gcccggcgag aaactgaaat cgatccggcg
    30061 agcgatacag cacattggtc agacacagat tatcggtatg ttcatacaga tgccgatcat
    30121 gagcgcgtac gaaacagacc gtgccaccgg tgatggtata gggctgccca ttaaacacat
    30181 gaatacccgt gccatgttcg acaatcacaa tttcatgaaa atcatgatga tgttcaggaa
    30241 aatccgcctg cgggagccgg ggttctatcg ccacggacgc gttaccagac ggaaaaaaat
    30301 ccacactatg taatacggtc atactggcct cctgatgtcg tcaacacggc gaaatagtaa
    30361 tcacgaggtc aggttcttac cttaaatttt cgacggaaaa ccacgtaaaa aacgtcgatt
    30421 tttcaagata cagcgtgaat tttcaggaaa tgcggtgagc atcacatcac cacaatgaag
    30481 ctaattgtga acatcatcac gttcatcttt ccctggttgc caatggccca ttttcctgtc
    30541 agtaacgaga aggtcacgaa ttcaggcgct ttttagactg gtcgtaatga aattcagcag
    30601 gatcacatta tgacctttcg caattgtgtc gccgtcgatc tcggcgcatc cagtgggcgc
    30661 gtgatgctgg cgcgttacga gcgtgaatgc cgcagcctga cgctgcgcga aatccatcgt
    30721 tttaacaatg gcctgcatag ccagaacggt tatgtcacct ggaatgtgga tagcctggaa
    30781 agtgccattc gccttggatt aaacaaggtg tgcgaggaag ggattcgtat cgatagcatt
    30841 gggattgata cctggggcgt ggactttgtg ctgctcgacc aacagggtca gcgtgtgggc
    30901 ctgcccgttg cttatcgcga tagccgctcc aatggcctaa tggcgcaggc acagcaacaa
    30961 ctcggcaaac gcgatattta tcaacgtagc ggcatccagt ttctgccctt caatacgctt
    31021 tatcaactgc gtgcgctgac ggagcaacaa cctgaactta ttccacacat tgctcacgct
    31081 ctgctgatgc cggattactt cagctatcgc ctgaccggca agatgaactg ggagtacacc
    31141 aacgccacga ccacgcaact ggtcaatatc aatagcgacg actgggacga gtcgctactg
    31201 gcgtggagcg gggctaacaa agcctggttt ggtcgcccga cgcatccggg taatgtcatt
    31261 gggcactgga tttgcccgca gggtaatgag attccggtgg tcgccgttgc cagccatgat
    31321 accgccagcg cggttatcgc ctcgccgtta aacggttcac gcgctgctta tctctcttct
    31381 ggcacctggt cattgatggg cttcgaaagc cagacgccat ttaccaatga cacggcgctg
    31441 gcagccaaca taaccaatga aggcggggcg gaaggtcgct atcgggtgct gaaaaatatt
    31501 atgggcttat ggctgcttca gcgagtgctt caggagcggc aaatcaacga tctcccggcg
    31561 cttatcgccg cgacacaggc acttccggcc tgccgcttca tcatcaatcc caatgacgat
    31621 cgctttatta atcctgaggc gatgtgcagc gaaattcagg ctgcgtgtcg ggaaatggcg
    31681 caaccgctcc cggaaagtga tgctgaactg gcgcgctgca ttttcgacag tctggcgctg
    31741 ctgtatgccg atgtgttgca tgagctggcg cagctacgcg gtgaagattt ctcgcaactg
    31801 catattgtcg gcggcggctg ccagaacacg ctgctcaacc agctatgtgc cgatgcctgc
    31861 ggtattcggg tgatcgccgg gcctgttgaa gcctcgacgc tcggcaatat cggcatccag
    31921 ttaatgacgc tggatgaact caacaatgtg gatgatttcc gtcaggtcgt cagcaccacc
    31981 gcgaatctga ccacctttac ccctaatcct gacagtgaaa ttgcccacta tgtggcgcag
    32041 cttcactcga cacgacagac aaaggagctt tgcgcatgac cactcaactg gaacaggcct
    32101 gggagctggc gaaacagcgt ttcgcggcgg tggggattga tgtcgaggag gcgctgcgcc
    32161 aacttgatcg tttacccgtt tcaatgcact gctggcaggg cgatgatgtt tccggttttg
    32221 aaaacccgga aggttcgctg accggtggga ttcaggctat tggtaattat ccgggcaaag
    32281 cgcgtaatgc cagtgagcta cgagccgatc tggaacaggc tatgcggctg attccggggc
    32341 cgaaacggct taatttacat gccatctatc tggaatcaga cacgccagtc tcgcgcgacc
    32401 agatcaaacc tgagcacttc aaaaactggg ttgaatgggc gaaagccaat cagctcggtc
    32461 tggattttaa cccctcctgt ttctcgcatc cgctaagcgc cgatggcttt acgctctcgc
    32521 atcccgacga cagcattcgc cagttctgga ttgatcactg caaagccagc cgtcgcgttt
    32581 cggcctattt tggcgagcaa ctcggcacac catcggtgat gaacatctgg atcccggatg
    32641 gtatgaaaga tatcaccgtt gaccgtctcg ccccgcgtca gcgtctgctg gcagcactgg
    32701 atgaggtgat cagcgagaag ctggatccgg cgcaccatat cgacgccgtt gagagcaaat
    32761 tgtttggcat tggcgcagag agctacacgg ttggctccaa tgagttttac atggggtatg
    32821 ccaccagccg ccagactgcg ctgtgcctgg acgccgggca tttccacccg actgaagtga
    32881 tttccgacaa gatttccgcc gccatgctgt atgtaccgca gttgctgctg cacgtcagcc
    32941 gtccggttcg ctgggacagc gatcacgtgg tgctgctgga tgatgaaacc caggcgattg
    33001 ccagtgagat tgttcgtcac gatctgttcg accgggtgca tatcggcctc gacttctttg
    33061 atgcctctat caaccgcatt gccgcgtggg tcattggtac acgcaatatg aaaaaagccc
    33121 tgctgcgtgc gttgctggaa cctaccgctg agctgcgcaa gctggaagcg gcgggcgatt
    33181 acactgcgcg tctggcgcta ctggaagaac agaaatcgtt gccgtggcag gcggtctggg
    33241 aaatgtattg ccaacgtcac gatacgccag caggtagcga atggctggag agcgtgcggg
    33301 cttatgagaa agcgattttg agccagcgtg ggtaaacact gccggatgcg gcgcgagcgc
    33361 cttatccggc ctacaggtcg aatgaccatg cgtgctgcca ccctcacccc ggccctctcc
    33421 ctgaaagggc gagggagaaa aacatagcct cgtgcggcaa cggagctgaa caccgctccg
    33481 gacagtcccc tcgcccctac ggggagaggg ttagggtgag gggaacaagc cgcaccatgt
    33541 ctaaacaagg caacctaatt tacaggaaca cagaacatgc aaaacattac ccagtcctgg
    33601 tttgtccagg gaatgatcaa agccaccacc gatgcctggc tgaaaggctg ggacgagcgc
    33661 aatggtggta acctgacgct gcgcctggat gacgccgata tcgcgcctta tcacggcaac
    33721 tttcatgcac aaccgcgcta tattcccctc agccaaccca tgcctttact ggcaaatacg
    33781 ccgtttattg tcaccggctc cggcaaattc ttccgtaacg tccagcttga tcctgcagct
    33841 aacttaggcg tcgtaaaagt cgacagcgac ggcgcgggct accatattct ctgggggtta
    33901 accaacgaag ccgttccaac ttccgaactt ccggctcact tcctttccca ctgcgagcgc
    33961 attaaagcca ccaacggtaa agatcgggtg atcatgcact gccacgccac caacctgatc
    34021 gccctcacct atgtacttga aaacgacacc gcggtcttta ctcgccaact gtgggaaggc
    34081 agcaccgagt gtctggtggt attcccggat ggcgttggca ttctgccgtg gatggtgccc
    34141 ggcacggacg aaatcggcca ggcgaccgca caagagatgc aaaaacattc gctggtgttg
    34201 tggcccttcc acggcgtctt cggcagcggg tcgacgttgg atgaaacatt cggtttaatc
    34261 gacaccgcag aaaaatcagc acaagtatta gtgaaggttt attcgatggg cggcatgaaa
    34321 cagaccatca gccgtgaaga gttgatagcg ctcggcaagc gcttcggcgt tacaccacta
    34381 gctagtgcgc tggcgctgta aggagcaaac atgatccgca aagcctttgt catgcaggtt
    34441 aaccccgacg cccacgaaga gtatcagcgt cggcataacc ccatctggcc ggaactggaa
    34501 gcagtgttga aatctcacgg tgcgcataac tacgccattt acctcgacaa agcgcgtaat
    34561 ctgctgtttg ccacggtaga gattgaatct gaagaacggt ggaatgcggt agccagcact
    34621 gatgtttgcc aacgttggtg gaaatatatg accgatgtta tgcccgctaa cccggataac
    34681 agcccggtga gtagcgagct gcaagaagtg ttttacctgc cgtaattgcc caatgtgcca
    34741 gagatgcctc cggcacaata ttttaaattt cgatttgaaa aattaaacat attacatctc
    34801 ccggtaagtt atatttccct gatacattgt gagtaaatca caaaaataat gaataaccca
    34861 ttaatgattc atgtggttta tttaaataac cattatgtgc attactccgc aaatctgacc
    34921 tttcactctg tctcaaattg tcattgttta ccgacatatc gcctctcatc acttaatcgc
    34981 tccaccatta caattgatta aaatatattt tatggagtcc gattaatggc agctcttact
    35041 gcaagctgta ttgacctgaa tattcagggc aatggcgctt attccgttct gaagcagttg
    35101 gcgacaatag cgttacaaaa cggttttatc accgactcac accagttcct gcaaaccctg
    35161 ctgctgcgcg aaaaaatgca ctctactgga tttggttccg gtgtcgccgt gccgcacggt
    35221 aaaagcgcct gcgttaaaca accgtttgta ttattcgccc gcaaagcgca ggctattgac
    35281 tggaaagcca gcgatggcga agacgtcaat tgctggatct gcctcggcgt gccgcaaagc
    35341 ggcgaagagg atcaggtcaa aatcatcggc acactgtgtc gcaaaattat ccaccaggat
    35401 tttattcatc aactgcaaca gggcgacgcc gaccaggtgc ttgccttgtt aaatcaaacc
    35461 ctcagctcat aaggaagtgg cgatggagtc atccttatgt attgtcgcga tcaccaactg
    35521 ccccgccggg atcgctcaca cctacatggt ggcggaagcc ctggaacaga aagcccgttc
    35581 tctcggccat accataaaag tggaaactca agggtccagt ggcgttgaaa accgcttatc
    35641 cagcgaagag attgccgctg ccgattacgt cattctcgct accgggcgtg gcctgagcgg
    35701 tgatgatcgc gcgcggtttg ccgggaagaa agtttatgag attgccatct cccaggcgtt
    35761 gaaaaatatc gaccagattt tcagcgaatt accgacaaac tcgcagcttt ttgccgcaga
    35821 tagcggcgtg aagcttggca aacaggaagt gcaaagcggc agcgtgatga gccacctgat
    35881 ggcgggcgtc tctgcggcgc tgccgtttgt catcggcggt ggtatcctgg tggcgcttgc
    35941 caacatgctg gtgcagttcg gtttgccgta taccgatatg tcgaaaggcg cgccgtcgtt
    36001 tacctgggta gttgaatcca tcggttacct tggcttcacc tttatgatcc ccatcatggg
    36061 cgcttatatt gcctcgtcga ttgccgataa accggctttc gctccggcgt ttctggtttg
    36121 ctatctggcg aacgacaaag cgctgctcgg cacccagtcg ggtgcgggat tcctcggcgc
    36181 ggtggtgctg gggctggcga ttggctattt cgtcttctgg ttccgcaaag tgcgccttgg
    36241 caaagcatta caaccgctgc tcggttcaat gctgatcccg ttcgttactc tgctggtttt
    36301 cggcgtgctc acttactacg ttatcgggcc ggtgatgtcc gacctcatgg gcgggctgct
    36361 ccacttcctg aataccattc ctccatcaat gaagtttgcg gcagcgtttc tggtcggtgc
    36421 gatgctggcg ttcgatatgg gcggccccat caataaaacc gcctggttct tctgcttctc
    36481 actgctggaa aaacacattt atgactggta cgccatcgtc ggcgttgtcg cgttgatgcc
    36541 gcccgttgcg gcgggtcttg cgacgttcat tgcgcccaaa ctgtttaccc gacaggaaaa
    36601 agaggctgcc agtagtgcca ttgtggtcgg tgccacagtc gcaaccgaac cggctatacc
    36661 ttacgctctg gcagctccgt tgccgatgat taccgccaat acgctggcgg gtggcattac
    36721 cggcgtgctg gtgatcgctt tcggaatcaa acgtctggca cctggtttgg gtatctttga
    36781 cccgctgatc ggcctgatgt cgccggtggg ttcgttttat ctggtgctag cgattggcct
    36841 cgcgctgaac atttcattca ttatcgtcct gaaaggattg tggttacgcc gcaaagcgaa
    36901 agccgcgcaa caggagcttg tccatgaaca ttgagttact gcaacagttg tgcgaagcca
    36961 gcgccgtcag cggcgatgaa caggaagttc gcgacattct gataaacacg ctggaacctt
    37021 gcgttaatga gatcaccttt gatggtctgg gcagctttgt tgcccgtaag gggaataaag
    37081 gtccaaaagt tgccgttgtc aggcatatgg atgaagtcgg ctttatggtc acccacatcg
    37141 acgagagcgg ttttctgcgc tttaccacca ttggcggctg gtggaatcag tcaatgctca
    37201 accaccgggt aaccatacgc acacacaagg gatttaaaat ccctggtgtg attggttccg
    37261 tcgcacctca tgcgttaacg gaaaagcaaa agcaacagcc gctgtcattt gatgagatgt
    37321 tcattgatat tggcgcgaac agtcgcgatg aggtggaaaa acgcggtgtg gcgatgggcg
    37381 attttattag cccggaagcc aattttgcct gctggggcga agataaagtg gtcggcaagg
    37441 cgctggataa ccgcatcggc tgcgcaatga tggctgaact attgcagacg gtgaataatc
    37501 ccgaaattac gctgtatggc gtcggcagcg tggaagaaga agttgggcta cgcggggcac
    37561 aaacctcggc ggaacacatt aaaccggatg tggtgattgt gctggatacc gctgtcgcag
    37621 gtgatgttcc gggcattgat aacattaaat acccgctgaa actgggccag gggccggggc
    37681 tgatgctgtt tgacaagcgc tacttcccca accagaaact ggtggcggcg ttaaaaagct
    37741 gtgccgcaca taacgattta ccgctgcaat tttccaccat gaaaaccggt gcgacggatg
    37801 gcgggcgcta caacgtgatg ggcggcgggc gtccggttgt tgcgctgtgc ctgccaaccc
    37861 gctatctgca cgctaacagc ggcatgattt caaaagccga ttacgatgct ctgctcacgt
    37921 tgatacggga ttttctgacc accttaaccg cggagaaagt caacgcgttt agccagttcc
    37981 gtcaggtgga ttaatgctaa acgaacgcca gttaaagatt gtcgatctgc tggagcaaca
    38041 gccgcgcacg cctggtgagc tggcgcaaca gactggcgtt tcaggcagga ccatcctgcg
    38101 tgatattgac tatctcaact ttacccttaa cggcaaagcc cgcattttcg ccagtggcag
    38161 tgcgggctat cagctggaaa tcttcgagcg ccgcagcttt tttcagttgc tgcaaaagca
    38221 cgataacgac gatcggctgc tggcgctgtt attactgaat acctttactc cccgtgcgca
    38281 actcgcctcg gcgcttaatt tgccagaaac ctgggtagca gagcgtctgc cccggttaaa
    38341 acagcgttat gaacgcactt gttgcctggc cagtcgcccc ggtttgggcc atttcattga
    38401 tgagacagaa gagaaacgcg ttatcttgct ggcgaacttg ctgcgcaaag atccgttttt
    38461 aattccgctg gcgggcgtaa cacgagacaa ttttcagcat ttatccacgg cctgcgacaa
    38521 ccaacaccgc tggccgctca tgcagggtga ttatctctcc agcctgattc tggcgattta
    38581 cgccctgcgt aatcaactga ccgatgagtg gccgcaatat cccggtaacg agataaaaca
    38641 aatcgttgaa cagagcggca tgtttcttgg tgataacgct gtaagaaccc tgacaggttt
    38701 gatagagaaa cagcatcagc aagcgcaggt aatttcagcc gatcatgtgc tggggttgct
    38761 gcaaagggtg ccaggcatcg cgtcattgaa tattattgat acgcagctgg ttgagaatat
    38821 taccgggcat ttattacgtt gccttgccgc accggtgtgg attgccgagc accgccaaag
    38881 cagcatgaat aacctgaaag ccgcctggcc tgcggccttt gatatgagtc tgcactttat
    38941 tacgctactg cgtgaacagc tcgatattcc ccttttcgat agcgatctga tcggtttgta
    39001 ttttgcctgt gcgctggaac ggcatcaaaa cgaacgccag ccgatcattt tgctctcgga
    39061 ccagaacgcg attgccacta ttaatcagct cgccattgag cgcgatgttt tacattgtcg
    39121 ggtgattatt gcccgtagct taagcgagct tattgccatt cgcgaagaga ttgagccgtt
    39181 attgatcatt aacaacagcc attatttact ggatgacgcg gtaaataatt acatcaccgt
    39241 aaaaaatatc attacggctg ccgatatcga acaaataaaa cactttctgg cgacggcatt
    39301 tattcgccaa cagccggaac gctttttctc tgcccccgga agttttcatt attcgaatgt
    39361 acgcggtgaa agctggcaac atattacccg gcaaatttgt gcgcaattag tggcacaaca
    39421 ccatattacc gccgatgaag cgcaacggat catcgcccgc gaaggcgaag gtgaaaacct
    39481 gattgttaat cgcctcgcca tcccacattg ctggagcgaa caggagcgac gttttcgtgg
    39541 attttttatt accctcgccc aaccagttga ggtgaataac gaagtcatta accatgtctt
    39601 gatcgcctgc gccgccgccg atgcgcgtca tgagctaaaa atatttagct atctggcaag
    39661 cgtattgtgt cagcatccgg cagaagttat tgccgggtta acaggatatg aggcatttat
    39721 ggagttactt cacaaggggt gaactgtgtc ggatgcgccg catccgacac tgcattttca
    39781 tcaaaaacgt ttaacgcagg ctattatagt catcaaccat ttcgttgtac tcgcgtaacg
    39841 ctcgtggaaa tgagtcttcg accatccagc ttgtgtttgc atcctgaaga tgctctttat
    39901 cccagtcaga gtacgggact ttatctctga cgcggcgaac atatttttta gtctcgagtt
    39961 gatactggtc tgccgaatta atgaacgaga aattcatacc gcctttatcc gcttctttgg
    40021 cctgcgctac cagcgtttcc agttcagaga ctttcttcat tgccgcatca acatcaa
//
LOCUS       DADMKF010000019        39910 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.019, whole genome shotgun sequence.
ACCESSION   DADMKF010000019 DADMKF010000000
VERSION     DADMKF010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39910
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.019"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(174..849)
                     /locus_tag="JNC24_000925"
                     /pseudo
     CDS             complement(174..849)
                     /locus_tag="JNC24_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(994..1602)
                     /locus_tag="JNC24_000926"
     CDS             complement(994..1602)
                     /locus_tag="JNC24_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HAY9157745.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVIRNKKPYVFEAVGPVKYPPLKQWIAHGEKGKYVVRRVEGGLSVEQQQK
                     LAQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            complement(1786..2103)
                     /gene="metJ"
                     /locus_tag="JNC24_000927"
     CDS             complement(1786..2103)
                     /gene="metJ"
                     /locus_tag="JNC24_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HAY9157746.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            2380..3540
                     /gene="metB"
                     /locus_tag="JNC24_000928"
     CDS             2380..3540
                     /gene="metB"
                     /locus_tag="JNC24_000928"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HAY9157747.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDAVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLRRFLSGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            3543..5975
                     /locus_tag="JNC24_000929"
     CDS             3543..5975
                     /locus_tag="JNC24_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HAY9157748.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPGKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTDFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            6324..7214
                     /gene="metF"
                     /locus_tag="JNC24_000930"
     CDS             6324..7214
                     /gene="metF"
                     /locus_tag="JNC24_000930"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HAY9157749.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            7543..9723
                     /gene="katG"
                     /locus_tag="JNC24_000931"
     CDS             7543..9723
                     /gene="katG"
                     /locus_tag="JNC24_000931"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HAY9157750.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSERVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            9817..10722
                     /gene="yijE"
                     /locus_tag="JNC24_000932"
     CDS             9817..10722
                     /gene="yijE"
                     /locus_tag="JNC24_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HAY9157751.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVMALALVSRKKKEAVSVKRI"
     gene            complement(10749..11366)
                     /locus_tag="JNC24_000933"
     CDS             complement(10749..11366)
                     /locus_tag="JNC24_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HAY9157752.1"
                     /translation="MKASLALLSLLTAFTSHSLKSPAVPPTVVQIQANTNLAIADGAR
                     QQIGSTLFYDPAYMQLTYPGGDVPQKRGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     EYPQKWQLKRPDSNIDHRRVPNLETWFTHHDKTRPISKNPSDYQAGDIVSWRLDNGLA
                     HIGVVSDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFVK"
     gene            complement(11640..12743)
                     /gene="gldA"
                     /locus_tag="JNC24_000934"
     CDS             complement(11640..12743)
                     /gene="gldA"
                     /locus_tag="JNC24_000934"
                     /EC_number="1.1.1.6"
                     /EC_number="1.1.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
                     /protein_id="HAY9157753.1"
                     /translation="MDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFA
                     QSTVEKSFKDAGLVVEIAPFGGGCSQNEIDRLRGIAETAQCGAILGIGGGKTLDTAKA
                     LAHFMGVPVAIAPTIASTDAPCSALSVIYTDEGEFDRYLLLPNNPNMVIVDTKIVAGA
                     PARLLAAGIGDALATWFEARACSRSGATTMAGGKCTQAALALAELCYNTLLEEGEKAM
                     LAAEQHVVTPALERVIEANTYLSGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVA
                     FGTLTQLVLENAPVEEIETVAALSHAVGLPITLAQLDIKEDVPAKMRIVAEAACAECE
                     TIHNMPGGATPDQVYAALLVADQYGQRFLQEWE"
     gene            complement(12754..13416)
                     /gene="fsa"
                     /locus_tag="JNC24_000935"
     CDS             complement(12754..13416)
                     /gene="fsa"
                     /locus_tag="JNC24_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HAY9157754.1"
                     /translation="MELYLDTANVAEVERLARIFPIAGVTTNPSIIAASKESIWEVLP
                     RLQKAIGDEGILFAQTMSRDAQGMVEEAKRLRDAIPGIVVKIPVTSEGLAAIKILKKE
                     GITTLGTAVYSAAQGLLAALAGAKYVAPYVNRVDAQGGDGIRTVQELQALLEMHAPES
                     MVLAASFKTPRQALDCLLAGCESITLPLDVAQQMLNTPAVESAIEKFEHDWNAAFGTT
                     HL"
     gene            complement(13428..15929)
                     /gene="ptsP"
                     /locus_tag="JNC24_000936"
     CDS             complement(13428..15929)
                     /gene="ptsP"
                     /locus_tag="JNC24_000936"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HAY9157755.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLHTDRK
                     GNAKSALALIGTDTLAGDNCQLLISGADEQEAHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALSNLPAAKDVDAEQSTLE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLRQHLLAGVSEGLSCAEAIVAS
                     ANHFCEEFARSSSSYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQSAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRVWLTQQARTADGIRIEIAANIAHSVE
                     AQAAFGNGAEGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGG
                     DKPVDYLNIPAEANPFLGYRAVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSM
                     EEILWVKEKLAEAKQQLRNEHIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSN
                     DLTQYLLAVDRDNAKVTRHYNSLNPAFLWALDYAVQTVHRQGKWIGLCGELGAKGSVL
                     PLLVGLGLDELSMSAPSIPAAKARMAQLDSRECRKLLNQAMACRTSLEVEHLLAQFRM
                     TQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFST
                     GLGFSFAIPHSKSEHIEQSTISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIF
                     SRLARRIMHEEFRNALVNAASADAIASLLQHELEL"
     gene            16238..17317
                     /locus_tag="JNC24_000937"
     CDS             16238..17317
                     /locus_tag="JNC24_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HAY9157756.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGTWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIVGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            17332..17652
                     /locus_tag="JNC24_000938"
                     /pseudo
     CDS             17332..17652
                     /locus_tag="JNC24_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
     gene            17703..19999
                     /locus_tag="JNC24_000939"
                     /pseudo
     CDS             17703..19999
                     /locus_tag="JNC24_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
     gene            19965..20843
                     /locus_tag="JNC24_000940"
     CDS             19965..20843
                     /locus_tag="JNC24_000940"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HAY9157757.1"
                     /translation="MTSSAGQRISCNVVETRRDDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLKIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            20845..21186
                     /locus_tag="JNC24_000941"
     CDS             20845..21186
                     /locus_tag="JNC24_000941"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HAY9157758.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(21173..22024)
                     /locus_tag="JNC24_000942"
     CDS             complement(21173..22024)
                     /locus_tag="JNC24_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY9157759.1"
                     /translation="MYHGVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVAAGGWNFPQWKTPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTERSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(22239..23972)
                     /gene="cptA"
                     /locus_tag="JNC24_000943"
     CDS             complement(22239..23972)
                     /gene="cptA"
                     /locus_tag="JNC24_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HAY9157760.1"
                     /translation="MHSTEVQAKPLFSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWAASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQDVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLSAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            complement(24155..26806)
                     /gene="ppc"
                     /locus_tag="JNC24_000944"
     CDS             complement(24155..26806)
                     /gene="ppc"
                     /locus_tag="JNC24_000944"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HAY9157761.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            complement(27158..28309)
                     /gene="argE"
                     /locus_tag="JNC24_000945"
     CDS             complement(27158..28309)
                     /gene="argE"
                     /locus_tag="JNC24_000945"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HAY9157762.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASIGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDTLRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGVNAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPLPSMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELIIQVIHHFCWH"
     gene            28463..29467
                     /gene="argC"
                     /locus_tag="JNC24_000946"
     CDS             28463..29467
                     /gene="argC"
                     /locus_tag="JNC24_000946"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HAY9157763.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVELPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQVLQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            29475..30251
                     /gene="argB"
                     /locus_tag="JNC24_000947"
     CDS             29475..30251
                     /gene="argB"
                     /locus_tag="JNC24_000947"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HAY9157764.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSTLVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRHAEQLPALFNGMPMGTRILA"
     gene            30312..31685
                     /gene="argH"
                     /locus_tag="JNC24_000948"
     CDS             30312..31685
                     /gene="argH"
                     /locus_tag="JNC24_000948"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HAY9157765.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALSVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLDELQKFSPVIGEDVYPILSLQSCLDKRAAKGGVSPQQVAQAIA
                     FAQARLG"
     gene            31952..32869
                     /gene="oxyR"
                     /locus_tag="JNC24_000949"
     CDS             31952..32869
                     /gene="oxyR"
                     /locus_tag="JNC24_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HAY9157766.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVPMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGYFDKVLKQAV"
     gene            complement(32852..34252)
                     /gene="sthA"
                     /locus_tag="JNC24_000950"
     CDS             complement(32852..34252)
                     /gene="sthA"
                     /locus_tag="JNC24_000950"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HAY9157767.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGVRVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIEGCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            34573..35223
                     /gene="fabR"
                     /locus_tag="JNC24_000951"
     CDS             34573..35223
                     /gene="fabR"
                     /locus_tag="JNC24_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HAY9157768.1"
                     /translation="MMGIRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            35223..35582
                     /locus_tag="JNC24_000952"
     CDS             35223..35582
                     /locus_tag="JNC24_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HAY9157769.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMPVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(35622..36722)
                     /gene="trmA"
                     /locus_tag="JNC24_000953"
     CDS             complement(35622..36722)
                     /gene="trmA"
                     /locus_tag="JNC24_000953"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HAY9157770.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IELDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQCETIFVDPPRSGLDSETEKMVQAYPCILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      36836..37026
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            37091..38935
                     /gene="btuB"
                     /locus_tag="JNC24_000954"
     CDS             37091..38935
                     /gene="btuB"
                     /locus_tag="JNC24_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HAY9157771.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTANRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRVEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRGTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALEHNFTDAWSGFVRGYGYDNRTNYDAYYSPGSPLLDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNVIVGHGSIGAG
                     VDWQKQTTTPGTGYVEDGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKAPNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            38880..39737
                     /gene="murI"
                     /locus_tag="JNC24_000955"
     CDS             38880..39737
                     /gene="murI"
                     /locus_tag="JNC24_000955"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HAY9157772.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEAFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
BASE COUNT         9108 a        10031 c        11015 g         9756 t
ORIGIN      
        1 atggggggcg ggggaatgtc atatgcctat gaatatgatg taaaatataa tatatgtcat
       61 ttgagaaaaa agagcagaat gaggtaaaaa tctttggctg tgtattcagt ctggtaatca
      121 tccaaggaaa gcttatcaga agggtgatga ctccaattag tagaacatat attttcgata
      181 aaagcaccga tgactttttc atgaagttct acagaacgcg agaaacagat ggttctgcgg
      241 gccagacgtt tgattcgggt tctcagggtc agattgttac gctcaatgtg ctgagtaaaa
      301 atcttaccag tcagatgctt ctccttcggt aactctctag cgtaactacc ccattcgtca
      361 gtggtcaaca tgccgatggt aaaagggtaa gcagcgcccg caattcacga caggtttcgt
      421 cagtccgggg gccaaaagtg taggccagaa caccaccagt tttcgtgttg tacacgtacc
      481 agagccagtg ctgtcgcgct ttactgccga caaagctcca ttgctgacta agtttgcaga
      541 tgagtgccac atcagcatga gcgaccgggg atgacgttat tcttcgtggc gagagttttt
      601 taaagttcgg atgactgtat taatgccgat tttcagcgta cgggcagtat cgcgaacccc
      661 ggcaccgttg aaagtcattt cagcgatttg gtctttaact cccggtttac ggggctcgta
      721 acagtaactg agctgaaata cccggtgaca gtcacggccg cggaatcgct catgtctttt
      781 tgggttctgc ccgtgacggt aaacctgaac ggactggcaa cgggggcaat gaaggttaac
      841 tgtgaccatg aaacaacctt aaaagcgagt attatatcct taattcaact aattggaggc
      901 atcaccgttt accggagcga cagcttgcgg aaaggtcgtg caactacaaa gcaacaaaca
      961 gactacggcg ggaattattt ttttcatcat ccattaccac gaaaacagcg gccactcttt
     1021 ggcgactgtg gtgagttgtg gcgcgtcgaa aacggcttgt ggtgaaacta ccgtttcctc
     1081 aagcggaata tttttgccgt agcgttcttt gagcttagcc tggaccagcg ggttgctgag
     1141 atcgaactct ttcaacttct gctgctcgcc cacgcgcatt cccagcgcgt tctggtaaac
     1201 cttccacacc acttccgaac agtactggcg atcgtcgctc catgagaagc tgaaatcata
     1261 cggtttaccg agataacgtt ttgccgtttg cgccagtttt tgctgttgtt caacactcag
     1321 tccgccttca acgcggcgaa caacgtattt gcccttttca ccatgggcga tccactgctt
     1381 gagcgggggg tatttcaccg ggccgaccgc ttcaaaaacg tagggctttt tgttgcgtat
     1441 caccagcata ccggtgtggc tataatcgga atgggtcgcc agttggatcg ctttactttg
     1501 cgatgagcga gagatctgaa agatgatgtc gccggtttgt ggctgccagg caaaggcagg
     1561 tacagaaacc agcaggctga ggatcagcag cctgtttttc atagttaaac gtccatgtat
     1621 aaaaagcggt gggtcgcaga caacgtgctc gttgtttatg ccggatgcgg cgtgaacgcc
     1681 ttatccggcc tacaagttcg tgcaaattca ataaattgca atatgacgta ggcctgataa
     1741 gcgtagcgca tcaggcgatt ccactccgcg ccgctctttt ttgctttagt attcccacgt
     1801 ctccgggtta atccccatct cacgcatgat ctcttttgcc gcttccggga tttcgtcgct
     1861 gcgctcttta cgcagatcgg catcatccgg caaaggttgc ccggtaaagg catgcagaaa
     1921 cgcttcgcac agcagctcgc tgttggtagc gtgacgcagg ttgttcacct gacgacgcgt
     1981 gcgttcatcg gtgaggattt ttaacacctt aagaggaatg gaaaccgtaa tctttttgac
     2041 ttgttcactc ttcttgccgt gctcagcgta tgggctgata tattcgccgc tccattcagc
     2101 catgagatac ttaatcctct tcgtcaataa attgagacca gaccacacaa tttatgtggg
     2161 tactgaccgt aaacccgcat agtttaccgt acaggcgtta ccgtgacatc gtgtaatgca
     2221 cctgtcggcg tgataatgca tataatttta acggctattt gggatttgct caatctatac
     2281 gcaaagaagt ttagatgtcc agatgtattg acgtccatta acacaatgtt tactctggtg
     2341 cctgacattt caccgacaaa gcccaggaaa cttcatcaca tgacgcgtaa acaggccacc
     2401 atcgcagtgc gtagcgggtt aaatgacgac gaacagtatg gttgcgttgt ccctcccatc
     2461 catctttcca gcacctataa ctttaccgga tttaatgaac cgcgcgcgca tgattactcg
     2521 cgtcgcggca acccaacgcg cgatgtggtt cagcgtgcgc tggcagaact ggaaggcggt
     2581 gctggcgcag tacttactaa taccggcatg tccgcgattc acctggtaac gaccgtcttt
     2641 ttgaaacctg gcgatctgct ggttgcgccg cacgactgct acggcggtag ctatcgcctg
     2701 ttcgacagtc tggcgaaacg cggttgctat cgcgtgttgt ttgttgatca aggcgatgaa
     2761 caggcattac gggcagcgct ggcagaaaaa cccaaactgg tactggtaga aagcccaagt
     2821 aatccattgt tacgcgtcgt ggatattgcg aaaatctgcc atctggcaag ggaagtcggg
     2881 gcggtgagcg tggtggataa caccttctta agcccggcat tacaaaatcc gctggcatta
     2941 ggtgccgatc tggtgttgca ttcatgcacg aaatatctga acggtcactc tgacgcggtg
     3001 gctggcgtgg tgattgctaa agacccggac gttgtcactg aacttgcctg gtgggcaaac
     3061 aatattggcg tgacgggcgg cgcgtttgac agctatctgc tgctacgcgg gttgcgaacg
     3121 ctggtgccgc gtatggagct ggcgcagcgc aacgcgcagg cgattgtgaa atatctgcaa
     3181 acccagccgt tggtgaaaaa actgtatcac ccgtcgctgc cggaaaatca ggggcatgaa
     3241 attgccgcgc gtcagcaaaa aggctttggc gcaatgttga gttttgaact ggatggcgat
     3301 gagcagacgc tgcgtcgttt cctgagcggg ctgtcgttgt ttacgctggc ggaatcatta
     3361 gggggagtgg aaagtttaat ctctcacgcc gcaaccatga cacatgcagg catggcacca
     3421 gaagcgcgtg ctgccgccgg gatctccgag acgctgctgc gtatctccac cggtattgaa
     3481 gatggcgaag atttaattgc cgacctggaa aatggcttcc gggctgcaaa caaggggtaa
     3541 aaatgagtgt gattgcgcag gcaggggcga aaggtcgtca gctgcataaa tttggtggca
     3601 gtagtctggc tgatgtgaag tgttatttgc gtgtcgcggg cattatggcg gagtactctc
     3661 agcctgacga tatgatggtg gtttccgccg ccggtagcac cactaaccag ttgattaact
     3721 ggttgaaact aagccagacc gatcgtctct ctgcgcatca ggttcaacaa acgctgcgtc
     3781 gctatcagtg cgatctgatt agcggtctgc tacccgctga agaagccgat agcctcatta
     3841 gcgcttttgt cagcgatctg gaacgcctgg cggcgctgct cgacagcggt attaacgacg
     3901 cagtgtatgc ggaagtggtg ggccacgggg aagtatggtc ggcacgcctg atgtctgcgg
     3961 tacttaatca acaagggctg ccagcggcct ggcttgatgc ccgcgagttt ttacgcgctg
     4021 aacgcgccgc acaaccgcag gttgatgaag ggctttctta cccgttgctg caacagctac
     4081 tggtgcaaca tccgggcaaa cgtctggtgg tgaccgggtt tatcagccgt aacaacgccg
     4141 gtgaaacggt gctgctgggg cgtaacggtt ccgactattc cgcgacacaa atcggtgcgc
     4201 tggcgggtgt ttctcgtgta accatctgga gcgacgtcgc cggggtatac agtgccgacc
     4261 cgcgtaaagt gaaagatgcc tgtctgctgc cgttgctgcg tctggatgaa gccagcgaac
     4321 tggctcgtct ggcggctccc gttcttcacg cccgtacttt acagccggtt tctggcagcg
     4381 aaatcgacct gcaactgcgc tgtagctaca cgccggatca aggttccacg cgcattgaac
     4441 gcgtgctggc ctccggtact ggtgcgcgta ttgtcaccag ccacgatgat gtctgtttga
     4501 ttgagtttca ggtgcccgcc agtcaggatt tcaaactggc gcataaagag atcgaccaaa
     4561 tcctgaaacg cgcgcaggta cgcccgctgg cggttggcgt acataacgat cgccagttgc
     4621 tgcaattttg ctacacctca gaagtggccg acagtgcgct gaaaatcctc gacgaagcgg
     4681 ggttacctgg cgaactgcgc ctgcgtcagg ggctggcgct ggtggcgatg gtcggtgcag
     4741 gcgtcacccg taacccgctg cattgccacc gcttctggca gcaactgaaa ggccagccgg
     4801 tcgaatttac ctggcagtcc gatgacggca tcagcctggt ggcagtactg cgcaccggcc
     4861 cgaccgaaag cctgattcag gggctgcatc agtccgtctt ccgcgcagaa aaacgcatcg
     4921 gcctggtatt gttcggtaag ggcaatatcg gttcccgttg gctggaactg ttcgcccgtg
     4981 agcagagcac gctttcggca cgtaccgact ttgaatttgt gctggcaggc gtggtggaca
     5041 gccgccgcag tctgttgagc tatgacgggc tggacgccag ccgcgcgtta gccttcttca
     5101 acgatgaagc ggttgagcag gatgaagagt cgttgttcct gtggatgcgc gcccatccgt
     5161 atgatgattt agtggtgctg gacgttaccg ccagccagca gcttgctgat cagtatcttg
     5221 atttcgccag ccacggtttc cacgtcatca gcgccaacaa actggcggga gccagcgaca
     5281 gcaataaata tcgccagatc cacgacgcct tcgaaaaaac cgggcgtcac tggctgtaca
     5341 atgccaccgt cggtgcgggc ttaccgatca accacaccgt gcgcgatctg atcgacagcg
     5401 gcgatactat tttgtcgatc agcgggatct tctccggcac gctctcctgg ttgttcctgc
     5461 aattcgacgg tagcgtgccg tttaccgagc tggtggatca ggcgtggcag cagggcttaa
     5521 ccgaacctga cccgcgtgac gatctctctg gcaaagacgt gatgcgcaag ctggtgattc
     5581 tggcgcgtga agcaggttac aacatcgaac cggatcaggt acgcgtggag tcgctggtgc
     5641 ctgcccattg cgaaggcggc agcatcgacc atttctttga aaatggcgat gaacttaacg
     5701 agcagatggt gcaacggctg gaagcggccc gcgaaatggg gctggtgctg cgctacgtgg
     5761 cgcgtttcga tgccaatggc aaagcgcgtg tgggcgtgga agcggtgcgt gaagaccatc
     5821 cgctggcatc actgctgccg tgcgataacg tctttgccat cgaaagccgc tggtatcgcg
     5881 ataatccgct ggtgatccgt gggcctggcg ctgggcgcga cgtcaccgcc ggggcgattc
     5941 agtcggatat caaccggctg gcacagttgt tgtaatttca gaaatttaat aatgcccggt
     6001 actcatgttt tcgggtttat ggtttctaat gaaatatatt gaattatcat aggattaggc
     6061 cggattaagc gtttacgacg aatccggcaa gaagcaataa gtacatggtt agtttatatt
     6121 tgcagtccgg tttgctttgc ataccggatt ttctttttct taccatcctg aagttttttc
     6181 atcttccctg atttttcctc accatcattg gtcatttttc ggttgacgcc cttcggcttt
     6241 tccttcatct ttacatctgg acgtctaaac ggatagatgt gcacaacaca acatataact
     6301 acaagcgatt gatgaggtaa ggtatgagct tttttcacgc cagccagcgg gatgccctga
     6361 atcagagcct ggcagaagtc caggggcaga ttaacgtttc gttcgagttt ttcccgccgc
     6421 gtaccagtga aatggagcag accctgtgga actccatcga tcgccttagc agcctgaaac
     6481 cgaagtttgt atcggtgacc tatggcgcga actccggcga gcgcgaccgt acgcacagca
     6541 ttattaaagg cattaaagat cgcactggtc tggaagcggc accgcatctt acttgcattg
     6601 atgcgacgcc cgacgagttg cgcaccattg cacgcgacta ctggaataac ggtattcgtc
     6661 atatcgtggc gctgcgtggc gatctgccgc cgggaagtgg taagccagaa atgtatgctt
     6721 ctgacctggt gacgctatta aaagaagtgg cagatttcga tatctccgtg gcggcgtatc
     6781 cggaagttca cccggaagca aaaagcgctc aggcggattt gcttaatctg aaacgcaaag
     6841 tcgatgccgg agccaaccgc gcgattactc agttcttctt cgatgtcgaa agctacctgc
     6901 gttttcgtga ccgctgtgta tcggcgggca ttgatgtgga aattattccg ggaattttgc
     6961 cggtatctaa ctttaaacag gcgaagaaat ttgccgatat gaccaacgtg cgtattccgg
     7021 cgtggatggc gcaaatgttc gacggcctgg atgatgatgc cgaaacccgc aaactggtgg
     7081 gcgcgaatat tgccatggat atggtgaaga ttttaagccg tgaaggagtg aaagatttcc
     7141 acttctatac gcttaaccgc gctgaaatga gttacgcgat ttgccatacg ctgggtgttc
     7201 gacctggttt ataaatagtg tggcttttgt gaaaatcaca cagtgatcac aaattttaaa
     7261 cagagcacaa aatgctgcct cgaaatgagg gcgggaaaat aaggttgtca gccttgtttt
     7321 ctccctcatt acttgaagga tatgaagcta aaaccctttt ttataaagca tttgtccgaa
     7381 ttcggacata atcaaaaaag cttaattacg atcaatttga cctacatctc tttaaccaac
     7441 aatatgtaag atctcaacta tcgcatccgt ggattaattc aattataact tctctctaac
     7501 gctgtgtatc gtaacggtaa cactgtagag gggagcacat tgatgagcac gtcagacgat
     7561 atccataaca cgacagccac tggcaaatgc ccgttccatc agggcggtca cgaccagagc
     7621 gcgggggcgg gcacaaccac tcgcgactgg tggccaaatc aactccgtgt tgacctgtta
     7681 aaccaacatt ctaatcgttc taacccgctg ggtgaggact ttgactaccg caaagaattc
     7741 agcaaattag attactacgg cctgaaaaaa gatctgaaag ccctgctgac agaatctcag
     7801 ccgtggtggc cagccgactg gggcagctac gccggtctgt ttattcgtat ggcctggcac
     7861 ggcgcaggta cttaccgttc aatcgatgga cgcggcggcg cgggtcgtgg tcagcaacgt
     7921 tttgcaccgc tgaactcctg gccggataac gtaagcctcg ataaagcgcg tcgcctgttg
     7981 tggccaatca aacagaaata tggtcagaaa atctcctggg ccgacctgtt tatcctcgca
     8041 ggtaacgtgg cgctggaaaa ctccggcttc cgtaccttcg gttttggtgc cggtcgtgaa
     8101 gacgtctggg aaccggatct ggatgttaac tggggtgatg aaaaagcctg gctgactcac
     8161 cgtcatccgg aagcgctggc gaaagcaccg ctgggtgcaa ccgagatggg cctgatttac
     8221 gttaacccgg aaggcccgga tcacagcggc gaaccgcttt ctgcggcagc agctatccgc
     8281 gcgaccttcg gcaacatggg catgaacgac gaagaaaccg tggcgctgat tgcgggtggt
     8341 catacgctgg gtaaaaccca cggtgccggt ccgacatcaa acgtaggtcc tgatccagaa
     8401 gctgcaccga ttgaagaaca aggtttaggc tgggcgagca cttacggcag cggcgttggc
     8461 gcagatgcca ttacctctgg tctggaagtt gtctggaccc agacgccgac ccagtggagc
     8521 aactatttct tcgaaaacct gttcaaatat gagtgggtac agacccgcag tccggctggc
     8581 gcaatccagt tcgaagcggt agacgcaccg gaaattatcc cggatccatt cgatccgtcg
     8641 aagaaacgta aaccgacgat gctggtgacc gacctgacgc tgcgttttga tcctgagttc
     8701 gagaagatct ctcgtcgttt cctcaacgat ccgcaggcgt tcaacgaagc ctttgcccgt
     8761 gcctggttca aactgacgca cagggatatg gggccgaaat ctcgctacat cgggccggaa
     8821 gtgccgaaag aagatctgat ctggcaagat ccgctgccgc agccgatcta caacccgacc
     8881 gagcaggaca ttatcgatct gaaattcgcg attgcggatt ctggtctgtc tgttagtgag
     8941 cgggtatcgg tggcctgggc atctgcttct accttccgtg gtggcgacaa acgcggtggt
     9001 gccaacggtg cgcgtctggc attaatgccg cagcgcgact gggatgtgaa cgccgcagcc
     9061 gttcgtgctc tgcctgttct ggagaaaatc cagaaagagt ctggtaaagc ctcgctggcg
     9121 gatatcatcg tgctggctgg tgtggttggt gttgagaaag ccgcaagcgc cgcaggtttg
     9181 agcattcatg taccgtttgc gccgggccgc gttgatgcgc gtcaggatca gactgacatt
     9241 gagatgttcg agctgctgga gccaattgct gacggtttcc gtaactatcg cgctcgtctg
     9301 gatgtttcca ccaccgagtc actgctgatc gacaaagccc agcaactgac cctgaccgcg
     9361 ccggaaatga ccgcgctggt gggcgggatg cgcgtactgg gtgccaactt cgatggcagc
     9421 aaaaacggcg tcttcactga ccgcgttggc gtattgagca atgacttctt cgtgaacttg
     9481 ctggatatgc gttacgagtg gaaagcgacc gacgaatcga aagagctgtt tgaaggccgt
     9541 gaccgcgaaa ccggcgaagt gaaatacact gccagccgtg cggatctggt atttggttct
     9601 aactctgtcc tgcgtgcggt ggctgaagtt tacgccagca gcgatgctca cgagaagttt
     9661 gttaaagact tcgtggcggc atgggtgaaa gtgatgaacc tcgaccgttt cgacctgctg
     9721 taatctgacc ttcttcagcg actgcctttc aggcagtcgc tgaagtttct ttaccggcgt
     9781 atagtgtcca caggaaaact acacactgga tctctcatgt ctgccgcagg aaagagcaac
     9841 ccactggcaa tcagtggcct ggttgtgctc acacttatct ggagttatag ctggattttc
     9901 atgaagcaag tcaccagcta catcggtgcc ttcgacttta ccgccttacg ctgcattttc
     9961 ggcgctctcg ttttattcat cgtcctttta ttacgtggtc gcggaatgcg cccgacaccg
    10021 tttaaataca ccttagccat tgccctgtta caaacctgcg ggatggttgg cctggcgcag
    10081 tgggcgctgg tcagcggcgg agcggggaag gtggcgatcc taagctatac catgccgttc
    10141 tgggtggtga ttttcgccgc gttgtttctc ggtgaacgcc tgcgacgtgg gcaatatttc
    10201 gcgatactga ttgccgcttt cggcttattt ttggtgttgc agccgtggca actcgatttc
    10261 tcttcgatga aaagtgccat gctggcaatc ctctccggcg tcagttgggg ggcgagcgcg
    10321 attgttgcta aacgtctgta tgcccgtcat ccgcgcgtgg atttattgtc gttaacatcc
    10381 tggcagatgc tctatgcggc gctggtgatg agtgtggtcg ctttactggt gccgcaacgt
    10441 gaaattgact ggcagcccac cgtgttctgg gcgctggcct acagtgcgat tctggcgacg
    10501 gcactggcgt ggagcttatg gttgtttgta ttgaaaaact tgcctgccag tattgccagc
    10561 ttaagcacac tggccgttcc cgtttgcggc gtactctttt cctggtggct gctcggcgag
    10621 aatccggggg ccgttgaagg tagcggtatt gtgctgattg tgatggcact ggcgctggtg
    10681 agccgtaaga aaaaagaagc cgttagtgta aaaaggatct gaattttttc ttcatgtggg
    10741 gcgatcgctt atttaacaaa ataacgataa tgccccacca tgcgccagct aaacagcaca
    10801 tcttcttcct gcgcacctgc gccaatgtta tgtatcacca gcggcgtacc gtcgcgggcg
    10861 aagccatctg aaaccacccc aatatgtgcc agcccgttat ctaatcgcca ggagacaata
    10921 tcgcccgctt gatagtcact ggggttcttg ctgatggggc gtgttttatc gtggtgggta
    10981 aaccaggttt ccagattagg cacccgacgg tgatcgatgt tgctgtccgg gcgctttaac
    11041 tgccattttt gcgggtactc ggcaaaattc ttcgccatat cttcgtgcac cagtttctgc
    11101 aaatcgacct tctggctgcg caatgcacgg atcaccacat cggaacatac cccgcgtttt
    11161 tgcgggacat caccgccagg ataggtaagc tgcatatacg ccggatcgta aaatagcgtg
    11221 ctgccgattt gctgtctggc accgtctgcg atggcaaggt tggtattggc ctggatttgt
    11281 accacggttg gtggaacggc gggagatttt aaggagtggc tggtaaatgc cgttagcagg
    11341 ctgagcagcg ccagtgaagc tttcatctgg ttcgtccgtg aaaataagag tcacagattc
    11401 gaccttccgg gataacgatt gttctgtaga gaaaatgtcc agaagcggat aaaaaactcc
    11461 tgaacaacgt gggagtttca gattactggc acagagtgcc ggatgcggcg tgaacgcctt
    11521 atctgtccta aaaaaaaaga aaattcaata tattgcagag attgcgtagg cctgataagc
    11581 gtagcgcatc aggcaatttt gcgttcaaac tcccggacaa gccgggagtt tggagtaggt
    11641 tattcccact cttgcaggaa acgctgaccg tactggtcgg ctaccagcag agcggcgtaa
    11701 acctgatctg gcgtcgcgcc gccaggcatg ttgtggatgg tttcacattc tgcacatgcc
    11761 gcttcagcca caattcgcat tttcgccggg acatcttctt taatatccag ttgagcgaga
    11821 gttattggca aacctaccgc atggctaagt gcagctacgg tttcgatttc ctcaaccggt
    11881 gcgttttcca gaaccagctg cgtcagcgta ccaaatgcca ctttttcacc gtgataatag
    11941 tgatgcgcgt ccgggatagc ggtcaggccg ttatgcactg cgtgcgccgc agccagacca
    12001 ccactttcaa aaccaacacc gctcaaatag gtgttcgctt caatcacgcg ctccagcgcc
    12061 ggagtcacta catgctgttc ggcagcaagc atcgcttttt cgccttcttc cagcagggtg
    12121 ttgtagcaca gttcagccag tgccagcgca gcctgggtgc acttgccgcc cgccatggtg
    12181 gtcgcgccgc tacgagagca ggcacgcgct tcaaaccagg ttgccagcgc atcgccgata
    12241 cccgccgcta acagacgtgc aggtgcgcca gcgacgattt tggtgtcgac aatgaccata
    12301 ttcgggttat ttggcaacag cagatagcgg tcaaactcac cctcatcggt gtagataaca
    12361 gacaatgcgc tgcacggtgc atcggtagag gcgatagtcg gtgcgatcgc taccggaaca
    12421 cccatgaaat gtgccagtgc tttggctgta tcgagggttt ttccgccacc gataccgaga
    12481 attgcgccac actgcgcagt ctccgcgatg ccacgcagac ggtcgatctc attttgcgaa
    12541 catccaccgc caaacggcgc aatttctact accagtccag catctttaaa gcttttctcg
    12601 acagtggatt gagcaaaacc taaaacaaat ttgtcaccca ccactaacca gcgttctgcc
    12661 agcggcttca ggtattcgcc cagacgatta atcacatcag cgccctggat gtatttaccc
    12721 ggtgattgaa taatgcggtc cataattgct cctttagaga tgagtagtgc caaatgcggc
    12781 attccagtcg tgttcgaact tctctatagc tgactctacc gcaggggtgt tgagcatttg
    12841 ttgcgctaca tctaagggca gggtgatgga ttcacatcct gccagtaaac agtccagcgc
    12901 ctgacgcggc gttttaaagc tggctgccag caccatgctt tctggcgcgt gcatttctaa
    12961 cagcgcttgc agctcctgaa ccgtacgaat gccgtctccg ccctgggcat ctacgcggtt
    13021 aacatacgga gcaacgtatt ttgcccctgc cagcgcggcc agtaaccctt gtgcagcact
    13081 gtacactgcg gtgccaagcg tagtaatacc ctcttttttc agtattttaa ttgctgccag
    13141 accttcggaa gtcaccggga ttttcaccac aatacccgga atagcgtcgc gcaggcgctt
    13201 cgcttcttcc accatcccct gcgcgtcgcg gctcatggtc tgagcaaaca gaatgccctc
    13261 atcaccaatc gctttttgca gacgcggcag cacttcccat atggactcct tgctggcagc
    13321 gataatgctc gggttagttg tcaccccggc aatggggaat atgcgtgcca gacgttcgac
    13381 ttctgcgacg ttagcggtgt ccagatacag ttccatgatg tttcctttta cagttccagt
    13441 tcatgttgca gcaggctggc gatagcgtcg gcagaggcgg cgttaaccag cgcgttacgg
    13501 aattcttcgt gcatgatgcg gcgagcgagg cgcgagaaaa tgcgcatgtg ctgatcgccc
    13561 gcagcgtgtt tgttcagggt taacatgatg atgaattgcg cttcatcatc accccagcgc
    13621 accggcgctt gcagacgcgc aacgctgatg gtggattgct caatgtgttc tgatttgctg
    13681 tgtggaatgg caaaactaaa gcccagaccg gtagagaaaa cggcctcgcg cgcccacaag
    13741 tcggcttcca gtttacgcgg atagcggcag cgccctgcca gcagcaggtt atcggtcatg
    13801 cctttgagca cttcttcttt gctgcgccag tcgctttcca gtgtgatgca ctcggcggtg
    13861 accagcggtg cgtcttgttg ggtcatgcgg aattgcgcca gcaggtgctc cacttccagc
    13921 gaagtacggc aggccattgc ctggttgagc aacttgcggc actcacggct atcaagttgc
    13981 gccatccgcg ctttcgccgc cggaattgat ggtgcgctca tgctgagttc atccagccct
    14041 aagccgacca gcaacggcag cacggaacct ttcgctccca gctcaccgca cagaccaatc
    14101 catttaccct ggcgatgcac cgtctgcacg gcgtaatcga gcgcccacaa gaatgccgga
    14161 ttcaggctgt tgtagtgacg agtaacctta gcgttatcgc gatccactgc cagcagatat
    14221 tgcgtcaggt cattactacc aatactaaag aaatcaatct cttcgcagca ttgatcgatg
    14281 atgaacatca ccgacggcac ttccagcata atgccgagct ggatcttctc atcaaacgga
    14341 atgtgttcgt tacgtagttg ctgtttggct tctgccagtt tttctttcac ccataagatc
    14401 tcttccattg aggagatcat cgggatcatg attttcaggc tgccgtgagc ggaggcgcgc
    14461 aggatcgagc gtagttgtgt ggtaaataac gacgcgtatt cttcataaat acgcacggcg
    14521 cgataaccga ggaacgggtt tgcctctgcg ggaatgttta gataatcaac gggtttgtcg
    14581 ccgccaatgt ccatagtgcg cacaataatg ctgcgtccgt tggcagactc cagcgcctga
    14641 caaaaaatgt tgtacagctc gctttcgccc ggtgcactgg tgcgatccat atagagcatt
    14701 tcagtgcgga acaaaccaac gccttccgca ccattgccga atgcggcctg cgcttccacg
    14761 gagtgagcga tgttagcggc aatttcaatg cggataccgt cagcggtacg ggcttgttgg
    14821 gtcagccaga cacgctgttg ctcacgcagg gcgtcctgta cgcgggcttc ttgctgataa
    14881 taacgagcta cggcttctcc tggctcaacc acaatcgccc cggcgttgcc gtcgatataa
    14941 atcgtttgat gctgccacgg agtgagggca tcaatatcca caccaaccag cgtcggaatg
    15001 ttgaacgaac gggcaaggat caccgtatgt gaggtggtgc cgccactttt cagcaacaga
    15061 cctttgaggt gatttttatc cagttcgagg aactggctgg gggtcagttc atcagccata
    15121 caaatggcgg actgcgtcag tttgcccggt gccgggaagc gttgctcacc gtagatttgc
    15181 tggagtaact ggaagcagac gtcgcgtacg tccagggcac gttcttgcag gtagctgctg
    15241 ctggaacggg cgaactcttc gcaaaagtga ttcgcactgg cgacaatcgc ttcggcgcag
    15301 cttaatccct cgctgacgcc tgccagtaag tgctgacgta aagatgtatc gccagccagg
    15361 gatcggtgag cttccagtat cgcgctggta gcaccgtcgc tatccagcag acgaaactca
    15421 atgtttttca gtaccagcgt caggccgttt tccagtgtgg attgctcggc gtcaacgtct
    15481 ttggctgcgg gaagattact cagcgcattg agatctaaag aagagatcgg cgtcagaatg
    15541 ccgcctgcac taccgctgca cacggtgcgg gcgcggataa tttgcggatt cagattggtc
    15601 agtgaaaccg gcagtggttc cagttcgtca gatttaactt ccgccagcgg cgcatcgcag
    15661 tgggggaatt catcgcgcag ccattggctt aaacgctggt gcgcttcctg ttcgtcggcc
    15721 ccggaaatca gtaactggca gttatcgccc gccagcgtat cggtgccaat cagcgcaagg
    15781 gcgcttttgg cgttgccctt gcggtcagtg tgcaggttat gccactcaat ttgtgatgaa
    15841 aaagtattac acagcgtttc aacgtggctt gccggacgcg catgtacgcc gttaggtagc
    15901 tcacaaataa attccacaat cagggccatt gcctctctcc cggtacggtt ttctgtttac
    15961 aggataagag cgcacggcaa cggcctgcca tgtgacaaat ctgccaaaag ctggacaaat
    16021 gtaatgtaac cgtcaatttg cgacgcgtct cacaagacgc cgttttgcgg catgcttccg
    16081 gtttatcgca agttatgagg cggatcgcat ttttgtactg atattacaaa agtccagtaa
    16141 atggcctttt tatccactgt ttgctccgcc tgcgattgcc tattgtttcc tccatcaaaa
    16201 atatggacat gggctttcgc aagcccggga gcagcttatg aatgagttgg tgcagatcct
    16261 gaaaaatacc cgtcagcatt taatgacggg cgtttcacac atgattccct tcgtggtatc
    16321 gggcggtatt ttgctggcgg tttccgtcat gttgtatggc aaaggcgcag tgccggatgc
    16381 cgtagccgat ccaaatctga aaaaactgtt tgatatcggc gttgcgggct tgacgctgat
    16441 ggtgcctttc ctcgccgctt acattggtta ttccattgca gaacgttctg cgctggctcc
    16501 atgcgctatc ggtacctggg ttggtaacag ctttggtgcg ggcttctttg gtgcactgat
    16561 cgccgggatt atcggcggca tcgtggtgca ttacctgaag aaaattccgg tgcataaagt
    16621 tctgcgctcg gtgatgccta tcttcattat tcctatcgtc ggcacactga ttaccgcagg
    16681 catcatgatg tggggcttgg gcgagcctgt aggggcgttg accaacagcc tgactcagtg
    16741 gcttcagggg atgcagcagg gcagcattgt tatgctggcg gtgatcatgg gtctgatgct
    16801 ggcgttcgat atgggcggtc cggttaacaa agtggcctat gccttcatgc tgatttgcgt
    16861 tgctcagggt gtttataccg tggtggccat cgctgccgtg ggtatttgta tcccgccgct
    16921 ggggatgggg ctggcgacgc tgattggtcg taaaaatttc tccgcagaag agcgcgaaac
    16981 cggcaaagcg gcactggtga tgggctgtgt tggcgttact gaaggggcga ttcctttcgc
    17041 cgctgccgat ccgctgcgtg tcattccttc catcatggtc ggttcagttt gtggtgcagt
    17101 aactgcggcg ctggtcggtg cgcagtgcta tgcaggctgg ggtggtctga ttgtgctgcc
    17161 ggtggttgaa ggcaagctgg gttatatcgc agcagtggct gtcggagcag tggtgacggc
    17221 tgtttgtgtg aacgtgctga aaagtctggc gcgtaaaaat gggtcttcga ctgatgaaaa
    17281 agaagacgac ctggatttgg attttgaaat taattaattg aggaacgagc catgacgaaa
    17341 attattgcag taaccgcatg tccttcaggt gttgcccata cctatatggc agcagaggcg
    17401 ctggaaagtg ccgcgaaagc gaaaggctgg gaagtgaaag tagaaaccta ggggtcaatt
    17461 ggtctggaaa atgaactgac tgcggaagat gtggcgagcg ctgatatggt gattctgacc
    17521 aaagatatcg gcatcaagtt tgaagagcgt tttgctggca agaccatcgt gcgcgtcaac
    17581 atcagcgatg cagtaaaacg tgctgacgcc attatgagca aaattgaagc tcacctggcg
    17641 caaaccgctt aagttcttct cccgctcgca agggcgggtt cgctttccca caggagttcc
    17701 tcatgacgaa tcgtatctct cgcctcaaaa ctgcactgtt tgccaatacc cgtgaaatct
    17761 cgctggagcg ggcgctgctt tataccgcca gccatcggca aaccgaaggc gaaccggtga
    17821 tattgcgccg ggcgaaagca acagcgtata tccttgaaca tgttgaaatt tcgattcgtg
    17881 atgaagaact gattgccggt aaccgcaccg taaaaccgcg cgccgggatt atgtcgccgg
    17941 aaatggaccc ttactggctg ctgaaagagc tggatcaatt cccgacgcgt ccgcaggacc
    18001 gctttgctat cagcgaagaa gataaacgta tctaccgtga agagttgttc ccgtactggg
    18061 aaaaacgttc gatgaaagat ttcatcaacg ggcagatgac agatgaagta aaagccgcga
    18121 ccaacacgca gattttcagc atcaaccaga cggataaagg ccaggggcac attattattg
    18181 attacccacg cctgttgaat cacggtctgg gtgagctggt ggcacagatg cagcaacatt
    18241 gtcagcaaca gccggagaat cacttttatc aggccgcgtt gttactgctg gaagcctcgc
    18301 agaaacacat tttgcgttac gccgaactgg cggaaacgat ggcggcaaac tgcacagatg
    18361 cacagcgtcg cgaagaactg ctgactattg cggagatctc tcgccataac gccgaacata
    18421 agccgcagac gttctggcag gcgtgccagt tattctggta catgaacatc attctgcaat
    18481 acgaatccaa cgccagttcg ctgtctttag ggcgcttcga ccagtatatg ttgccgttct
    18541 atcaggcatc tttaacccag ggcgaagatc cggcgttcct gaaagaactg ctcgaatctt
    18601 tatgggtgaa atgcaacgac atcgtgctgt tgcgctccac cagcagcgcg cgttatttcg
    18661 caggtttccc gaccggctat accgcactgc tcggcgggtt aaccgagaac ggacgtagcg
    18721 cggtgaacgt gctttcgttc ctttgccttg acgcctatca aagcgtgcaa ttaccgcaac
    18781 cgaacctcgg cgtgcgcact aacgccttga tcgacacgcc gttcctgatg aaaaccgccg
    18841 aaaccattcg cctcggcacc ggtattccgc aaatctttaa cgatgaagtg gtggtaccag
    18901 cgttcctcaa ccgtggcgtt tcgctggaag atgcgcgcga ctattccgta gtgggctgtg
    18961 tggaattatc tattcccggc agaacctacg gcttgcatga catcgcgatg tttaatctgc
    19021 tgaaagtgat ggaaatctgc ctgcatgaaa atgaaggcaa tgctgcgctg acttatgaag
    19081 gtttactgga gcagatccgc gccaagatca gccactacat caccctgatg gttgagggca
    19141 gcaatatttg tgatatcggc catcgcgact gggcacctgt accgctgctc tcgtctttta
    19201 tcagcgattg tctggaaaaa ggtcgcgata ttaccgatgg cggcgcgcgt tataacttct
    19261 ccggcgtaca ggggatcggt atcgccaacc tgagcgattc tctccatgcg ttgaaaggga
    19321 tggtttttga gcaacagcgt ttaagttttg acgaattgct gtcggtatta aaagccaact
    19381 ttgcaacgcc agaaggcgaa aaagtccgtg ctcgcttaat taaccgcttc gagaaatacg
    19441 gtaacgatat cgacgaggtg gataacatca gcgccgaact gttacgccac tactgcaaag
    19501 aagtggaaaa ataccagaac ccgcgcgcgg ttacttcacg ccgggatcgt ataccgtttc
    19561 tgctcacgtc ccgttgggat cggtggttgg cgcgacgcca gacggtcgtt ttgccgggga
    19621 acaactggca gacggcggct tgtcacctat gctgggccag gacgcacaag ggccaacagc
    19681 ggtactgaag tcagtcagta agctcgataa tacgctgctg tctaacggta cgttgctgaa
    19741 cgtgaaattc actccggcga ccctggaagg tgaagcggga ttacgcaaac tggccgactt
    19801 cttacgggcg tttacccagc ttaagttgca gcatattcag tttaacgtgg tgaacgccga
    19861 cacgttgcgg gaagcgcaac agcgcccaca agattatgcc gggctggtgg tgcgcgttgc
    19921 cggatacagc gccttctttg tcgaactgtc gaaggagatc caggatgaca tcatccgccg
    19981 gacagcgcat cagctgtaac gttgtggaaa cgcgccgcga tgatgtggcg cgcattttca
    20041 acattcagcg ttattcactg aatgacggcg agggcattcg tacggtggtc ttttttaaag
    20101 gctgtccgca tctttgcccg tggtgtgcta atccggagtc gatctccggc aaaatccaga
    20161 cggtacgcag agaggcgaaa tgtctgcact gtgcgaaatg tttgcgtgat gcggatgaat
    20221 gcccctccgg ggcgtttgaa cggattggtc gcgatatcag ccttgacgct ctggaacggg
    20281 aagtgatgaa agatgacatt ttctttcgca cgtccggcgg cggcgtcacg ctttctggcg
    20341 gcgaagtgtt aatgcaggcg gagtttgcta cccgtttttt acagcgactg cggctgtggg
    20401 gtgtgtcatg cgccattgaa actgccggag acgcgccagc aagcaagctg ttaccgctgg
    20461 cgaaattgtg cgatgaagtg ctgttcgatt taaaaattat ggacgcgact caggcgcggg
    20521 atgtggtgaa gatgaacctg ccacgcgtgc tggagaatct gcgtttgctg gtgagtgagg
    20581 gtgtcaacgt gatcccgcgt ttaccgttga tccctggttt cacgctcagc agggagaata
    20641 tgcagcaggc gctggatgta ctgatcccgc tgaaaatcag gcagatccat ctgttaccgt
    20701 ttcatcagta cggcgaaccg aaataccgcc tgctggggaa aacatggtcg atgaaagagg
    20761 tgcctgcgcc gtcgtcagcc gatgtgacaa cgatgcgcga aatggcagaa cgggccggat
    20821 ttcaggttac cgtgggaggt taaaatggca tacctggtgg cagtaaccgc ctgcgtaagc
    20881 ggcgtggcgc atacttatat ggcggcggaa cggctggaaa agttgtgcca gttagagaag
    20941 tggggcgtca gcattgaaac tcagggcgcg ctgggaacgg agaatcgttt agcggacgag
    21001 gatatccgtc gggcggatgt tgctctgttg attacggata tcgagcttgc cggtgccgag
    21061 cgatttgaac attgccgcta tgtgcaatgc agcatctacg cattcctgcg tgagccgcag
    21121 cgggtaatga gcgcggtgcg caaagtgctt tctgcgccgc agcaaaccca tcttattctg
    21181 gagtagtcgg tttttctgtc agttggctgt ggtactgccg gcgatattcc gacggcgagc
    21241 gttcggtgtt tttacgaaac agacggcaga agtagttgct gtcgacaaag ccgcaggcgt
    21301 gcgccacttc ttttaccttc aggtcatagc ctttcaataa cgtcttagcg tgctccagtc
    21361 gcgtgtgatt caggtattcg ttaaaaccaa tggcccccgt tttttgaaac aggtgcgaga
    21421 gatagtttgg tgaaatatag aatgcctgtg cgacagattc gcgggtaagc gcggaggcat
    21481 agcgttcgtc gatataatcg cgaatagctt caaatagtgc ctggctgcgt gaggcggtct
    21541 ggatttggct gccgagcaaa tcgcggcagt ggctaagcag gctggctacg ataagccttg
    21601 ccgtttgctg ctcctgcggc tgcatttgca tttcattgag cgtttgtagc agaaaagaac
    21661 ctatacgtgg gccgcgtcgg gcgacgtgtt gcttcgccag attttgatat tgtttgccat
    21721 cccattgcac gacgctgaag ccgagttgct gtttgccaaa cagcacgcta aaggtggtag
    21781 cgggtgtttt ccattgtggg aaattccagc caccagcggc aacgtacagc acatcgccgg
    21841 gaactaacgt tgctccagcg ccggtatcaa caaactcacc ttccagcaca atttccagcc
    21901 gtggaaaatc gacctgatac gcaagatcgg gcaccgggcc gttactgctg gcaaagtaaa
    21961 tctggcgcag ggacagcggg ccgttgatca ggcgggagag cagatagctg acgccgtgat
    22021 acatgttatt cccttaggta tgtagacggg aaaacagtaa ctggcattat taaaaaaggc
    22081 cacctgaaaa gtgtggcctg aggggagttc gatgtaaaca ttgcggcagt tgtattgccg
    22141 gatgtggcgt aaacgcctta tccagcctac cgccgcgatc tgtaggccgg ataagacgcg
    22201 tcaagcgtcg catccggcaa atagcgcctg gctgataatt actgattacc tacctggtcg
    22261 ccatacggca gagtgtcgta atcgatcagc gcgtttttct tgtacgggtt accaatccag
    22321 cgggtagttt ctttgaactg cggattcacc actgaacggg ttggatcgta accgtcgtaa
    22381 gataagcccg ccaaatctga ccaggtgtgg atcagttccg ccaggctgta tttacgatcg
    22441 acatcctgtg agaaatcacg gggatgagtc gcttgccatt tttccgacgt ccacagcagg
    22501 aacggaatgg tgtacatgtg gcgcgtcggg ttgtcttcgt tacgcccctg agttttgtgc
    22561 ggcggcgtgt cgtaaacctc ttcaccgtgg tcagagaagt aaaccaggaa tccgttcggg
    22621 tctgccgctt taaagtcttt aatcaggctg gcaaccacat gatcgttata caggttagcg
    22681 ttgtcataat cgttatatga ttccagctct tctgcgctta atcctggcgg aacatgatcg
    22741 gtattgccat caaacttgcc ctgattttcc gggtagcggt atttgtattt gatatgcgta
    22801 cccagcagat gaacgatgat cagtttcttc ggcgcagggt cattcagcac atcctggaac
    22861 ggcttcagca cgttggtgtc gtattcacgc gcactctgcg tacgttgctg gttcatgtag
    22921 tactgcttgt cggtctggcg cgaaaatacc gtcagcatgg tattgcgggc ggtcatcgtc
    22981 tgctggttgg tgatccagaa ggttttataa cccgcctgtt tcatcatgtt catcagcgac
    23041 ggctgcgtca gatacagatc cgggttcttt tcattggcaa aggtcagcgc ctgttgcagg
    23101 atttcaatgg tgtacggacg agacgtaact acgttattaa acacggtcag attcggatcg
    23161 gttttatgca gcgcatccag ctccggcgtg gtttcacgcg gataaccgta cagactcatg
    23221 cgtccgcgct gggtcgactc gccaatcacc agcactaaag tgcgcggttc gttacccgat
    23281 tcatctttga aattagccag tggcggcaag gcattatttt cattcagtaa ctttgttagc
    23341 gagtttagtt gctgacgata ctgataatag ccggtcagga attgccacgg tgcggcaggc
    23401 tccatacgcg aggccaggtt atccaacgtt ttctcaaacg gcttgttttt gataaacgta
    23461 ttcatggcga tcggatgcag aatcaagcca taaagcaggg caaaagagac aacataacgc
    23521 cacggctttg gaatatagac cgggcgcagg cgtgtccaca gcagaactgc caccgccgta
    23581 taggccagcg cgataagcac aattttcagg ctgaaatact ggcttaaata ctcgctggct
    23641 tcgttggtgt tggtttcgaa catcacaaac agaacgctct gcgagaactc ctgcccgtag
    23701 atgacgtagt agcatagcgc cgccagagag gccgcccata gcaccacgcc gattactgcg
    23761 gcaataattt taatccgctt cggaaagagg aataccggga tcaaccacag cgagctgaat
    23821 aacagcgagt cgcgaatgcc gttggtgcca ctataaccac tgatgtaaat aatggcctgt
    23881 agcagagtag agaaaaacca aaagtagagc agtgcccaac ccagggcttt ccagctaaaa
    23941 aggggtttag cctggacttc tgtggaatgc atagtaagaa cctgtcttga aaaaaatatc
    24001 gccgaatgta acgacaattc cttaaggata tctgaaggta tattcagaat ttgaataaaa
    24061 tgcagacaga aatatattga aaacgagggt gttagaacag aagtatttca gaaaaccctc
    24121 gcgcaaaagc acgagggttt gcagaagagg aagattagcc ggtattacgc atacctgccg
    24181 caatcccggc aatagtgacc attaacgctt gttcgacgcg aggatccggt tcctggcctt
    24241 ctttttctgc ctggcgggag cggtgcagca actcggcctg caatacgttc agcgggtcgg
    24301 tgtaaatatt ccgtagctga atagactctg caatccacgg cagatcggcc atcagatggg
    24361 aatcgttggc aatcgccagc accactttga tgtcttcttc ttgcaggttg cgtaactctt
    24421 tacctaacgg ccacagtgct ttgtctacca gacgttgatc atagtattcc gccaaccaca
    24481 ggtctgcttt ggcgaagacc atctccagca tgccgagacg cgtcgagaag aatggccaat
    24541 cgcggcacat ggcttccagc tcgctctgtt tgccatcttc gaccactttt tgcagcgccg
    24601 tacctgcacc cagccaggcg gggagcatca ggcggttttg cgtccaggca aaaatccacg
    24661 gtatggcgcg cagcgactcg acgccgccgg ttgggcgacg tttcgccgga cgtgaaccca
    24721 acggcagttt gcccaattct tgttccggcg tagcggagcg gaagtaaggc acaaaatctt
    24781 tgttttcacg tacgtagcca cggtagagat cgcaggagat gactgacagt tcatccataa
    24841 tgcgacgcca gctctctttc ggctcaggtg gtggcagcag gttggcttcc agaatcgccc
    24901 cggtgtaaag cgacaggctg ctgacggtga tttctggcag accatattta aagcggatca
    24961 tctcgccctg ttcagtcacg cgcaggccac ctttcaggct tcctggcggt tgtgacagca
    25021 gcgccgcatg ggcaggtgcg ccgccgcgac caatggaacc gccgcgaccg tggaacaacg
    25081 tcagctcaat acccgctttt tcgcaggttt tgattaatgc atcctgtgcc tgatattgcg
    25141 cccaggaagc tgccatcacg cccgcatctt ttgctgagtc ggaatagcca atcatcacca
    25201 tctgtttgcc ctgaatcagg ccgcgatacc agtcgatatt gagcagctgg gtcatgacat
    25261 cgttggcgtt gttcaggtca tcgagggttt caaacagcgg agcaaccggc atcgcaaacc
    25321 cgatacccgc ttctttcagc agcaggtgga cagccagtac gtcggacggc gttttcgcca
    25381 tcgagatcac gtaggcggca atggagcctt gcggcgcttc ggcaatcacc tggcaggtat
    25441 cgagcacttc gcgcgtttcg gcgcttggtt gccagttgcg cggcagaagc ggacgtttgg
    25501 agttcagttc gcggatcagg aacgcctgtt tgtcggcctc tgaccagctt tcgtagtcgc
    25561 cgataccgag gtagcgggtc agctcgccca gcgcttcggt atggcgggtg ctctcctgac
    25621 ggatatcaat acggaccagc ggtacgccga aacatttcac gcggcgcagg gtgtcgagca
    25681 gatcgccgtt ggcgataata cccatgccac acgcctgaag tgactggtag caagcgtaga
    25741 gcggttccca cagttcttcg ttttgtgtca gcagcccttc tggttttggc agttcttcgc
    25801 ctttcaggcg cgcttccagc catgcctgtg tcgccatcag gcgagaacgc aggtttttca
    25861 tcagatagcg atacggttct gcggcacctt cttcgccaac cagcgccagc agttcagggg
    25921 tcgcttcaac catcgacagt tcagaaacca gaacctgaat atctttcagg aacagatcgg
    25981 tggctttcca gcgactgagt agcaggacgt ggcgggtgat atcggcagtg acgttcgggt
    26041 tgccgtcgcg gtcaccgccc atccacgaag taaaacggac cggaacaaat tcgacgggca
    26101 gtttgtagcc gaggttctct tccagttgtt cgttcagttc gcgcaagtaa ttcggtacgc
    26161 cttgccacag gctgttttct actacggcaa agccccattt ggcttcatct accgggcttg
    26221 gacgcagctt acggatttca tcagtatgcc atgactgggc gattaactgg cgcaggcgac
    26281 gcatcagctg gttgtgttcg tagtcagcga tatctttgtt atcgagctgt tttaagcagg
    26341 cgttcacttc caccattttg tggatcagtg tacgacgggt aatttcggtt gggtgagccg
    26401 tgaggaccag ttccagcgac agcgattcca ctgctttttt gatggtgtct tcgctcagtt
    26461 ccggctggtt tttcagtttg cgcagggtgc gggcgatcac ttccgggttg ctggcagctt
    26521 cgcctttcgg cgaaatgctg tggtattgct cggcggtgtt ggccaggttc aggaactgac
    26581 taaacgcacg cgcaacgggc agcagctcgt cgttcgacaa attttgtaag gtggtgagca
    26641 actcctggcg gttagcatca ttgccagcgc gtgaagattt cgacaactta cggatagttt
    26701 ctacgcgttc aagaatgtgt tctcccaacg catccttgat ggtttctccc agcactttgc
    26761 cgagcatact gacattacta cgcaatgcgg aatattgttc gttcatatta ccccagacac
    26821 cccatcttat cgtttgatag ccctgtatcc ttcacgtcgc attggcgcga atatgctcgg
    26881 gctttgcttt tcgtcgtctt ttataaagcc acgtaaaagc ggtgacgtca aatgctgcga
    26941 aatcgcttca gcaaacgaat aaatagcagg aatttacgcc attaaattca cgacgcttta
    27001 aataagcgta acttatggaa atgttaaaaa atcgccatac ataacaccaa aggtgtaggt
    27061 cggataagat gcgcaagcat cgcatccgac attattgcgg caccggagtt tggcaacggt
    27121 gccggatgcg gcgcgagcgc cttatccggc ctacgtttta atgccagcaa aaatggtgaa
    27181 ttacctggat aatcaactca cgggtgggtt taataaaccg cgtctccaga tattcatcag
    27241 gctgatgagc ttgattgatg gaacccggac cgagcaccag cgtcgggcat aacgtttgaa
    27301 taaacggcgc ttcggtacag taattcacca cttcggtttt tgctccgagc aatttctcaa
    27361 ccacttcaac cagttgatga ttcggtgggc attcatagcc agggatcggc ggatgcagct
    27421 cgtcgaccgt cagacgaccc ggccagcgtt cgctcaccgg agccaatgca tcgttgagca
    27481 aaccattaag ttcattgagt gtcatgctag gcagcggacg aatatccata tgcaactcac
    27541 agcaagcgca aatacggtta gaagcgtcgc caccgtgaat atgcccgagg ttgagcgtag
    27601 ggtatggcac ggtaaatgct tcgtagtgat aacgttcttt caggttatcg cgcaattgca
    27661 aaatatgccc gatggcgtcg tgcattagtt cgatagcgtt aactccgcgt gctggatcgc
    27721 tggagtgccc cgactggccc tgaatacgga tggcgttaga gatatgacct ttatgtgcgc
    27781 gtaccggttg tagtgacgtc ggttcgccaa tgatggcgca atccgggcgc agggcggtag
    27841 tttcggcaaa ataacgcgct ccggccatac tggtttcttc atcagcagtc gccagaatgt
    27901 agagcggttt tttcagtttc gtgacgtcga catcgcgtag cgtatcaagg ataaacgcaa
    27961 aaaagccttt catgtcggcg gtgcctaagc cgtaaagctt gccgtcatgc tccgtcagtg
    28021 taaacggatc gcgcgtccag cgaccgtcat caaatggcac cgtatcggta tgccccgcca
    28081 gcaacaagcc gccagccccc tgtccgatac ttgccagcat attgaatttg ttgcgagttc
    28141 ctggcacagg ctgcacttcc acattgaagc ctaaatcttt aaaccagtcc gccagcagag
    28201 tgattaaatc tgcattgctt tgatcgagtg cctcttccgt ggcgcttatt gaaggtgtgg
    28261 caatcagagc acggtaaatc tcgataaatg gcggtaattt gtttttcatt gttgacacac
    28321 ctctggtcat gatagtatca atattcatgc agtatttatg aataaaaata cactaacgtt
    28381 gagcgtaata aaacccacca gccgtaaggt gaatgtttta cgtttaacct ggcaaccaga
    28441 cataagaagg tgaatagccc cgatgttgaa tacgctgatt gtgggtgcca gcggctacgc
    28501 tggcgcagag ctagtgacct atgtaaatcg ccatccgcat atgaacataa ccgctttgac
    28561 tgtttcagcg caaagcaatg atgcgggaaa gttaatctcc gatttgcatc cgcagctaaa
    28621 aggcatcgtt gagctgccgt tgcagccgat gtcggatatc agcgagttta gcccaggggt
    28681 ggacgtagtg tttctcgcca ccgcccatga agttagccac gatttagcgc cgcagtttct
    28741 tgaagcgggc tgcgtggtgt tcgacctttc cggtgcgttt cgtgttaacg acgccacctt
    28801 ctatgaaaaa tattacggct ttacccatca atacccggaa ctgctggaac aggcagccta
    28861 cggtctggcg gagtggtgcg gtaataaatt aaaagaagcg aatttgattg cggtgccagg
    28921 ctgttatccg acggcggcac agctggcgct gaaaccgttg attgatgccg atcttcttga
    28981 cctcaatcag tggccggtga tcaacgccac cagcggcgtg agcggtgcag gacgtaaggc
    29041 agcgatttca aatagttttt gtgaagttag cctgcaaccg tatggcgtct ttactcatcg
    29101 ccatcaacca gagatcgcca cacacctcgg tgctgacgtt atcttcaccc cacatctggg
    29161 caatttcccg cgcggcattc tcgaaaccat tacctgccgc ctgaaatcgg gtgtgaccca
    29221 ggcgcaagtc gcgcaagtgt tacagcaggc gtatgcccat aaaccgctgg tgcggctgta
    29281 tgacaaaggc gttccggcgc tgaaaaatgt cgttgggctg ccattttgcg atatcgggtt
    29341 tgccgttcag ggcgagcatc tgattattgt ggcgaccgaa gacaacttac tgaaaggcgc
    29401 ggcggcacaa gcggtacagt gcgccaatat tcgtttcggc tatgcggaaa cgcagtctct
    29461 tatttaaggg tgcaatgatg aatccattaa ttatcaaact gggcggcgta ctgctggata
    29521 gtgaagaggc gctggaacgt ctgtttagca cactggtgaa ttatcgtgag tcacatcagc
    29581 gtccgctggt gattgtgcac ggcggcggtt gcgtggtgga tgagctgatg aaagggctga
    29641 atctgccggt gaaaaagaaa aacggcctgc gggtgacgcc tgctgatcag atagacatta
    29701 tcaccggagc actggcggga acggcaaata aaaccctgtt ggcatgggcg aagaaacatc
    29761 agattgcggc cgtaggtttg tttctcggtg acggcgacag cgtcaaagtg acccagcttg
    29821 atgaagagtt aggtcatgtt ggactggcgc agccaggttc gcctaagctt atcaactcct
    29881 tgctggagaa cggttatctg ccggtggtca gctccattgg cgtaacagac gaagggcaac
    29941 tgatgaacgt caatgccgac caggcggcaa cggcgctggc ggcaacgctg ggcgcggatc
    30001 tgattttgct ctccgacgtc agcggcattc tcgacggcaa agggcaacgc attgccgaaa
    30061 tgaccgccgc gaaagcagaa caactgattg agcagggcat tattactgac ggcatgatag
    30121 tgaaagtgaa cgcggcgctg gatgcggccc gcacgctggg ccgtccggta gatatcgcct
    30181 cctggcgtca tgcggagcag cttccggcac tgtttaacgg tatgccgatg ggtacgcgga
    30241 ttttagctta agttttgttg gccggaggcg cagctttccg gcattgaatt tcaaaataag
    30301 gaaacagagt tatggcactt tggggcgggc gttttaccca ggcagcagat caacggttca
    30361 aacaattcaa cgactcactg cgctttgatt accgtctggc ggagcaggat attgttggct
    30421 ctgtggcctg gtccaaagcc ctggtcacgg taggcgtgtt aaccgcagaa gagcaggcgc
    30481 aactggaaga ggcgctgagc gtgctgctgg aagatgttcg cgccaggcca caacaaatcc
    30541 ttgaaagcga cgccgaagat atccatagct gggtggaagg caaactgatc gacaaagtgg
    30601 gccagttagg caaaaagctg cataccgggc gtagtcgtaa cgatcaggta gcgactgacc
    30661 tgaaactgtg gtgcaaagat accgttagcg agttactgac ggctaaccga cagctgcaat
    30721 cggcgctggt ggaaaccgca caaaacaatc aggacgcggt catgccaggt tacactcacc
    30781 tgcaacgcgc ccagccggtg acgttcgcgc actggtgcct ggcctatgtt gagatgctgg
    30841 cgcgtgatga aagccgtttg caggatgcgc ttaagcgtct ggatgtcagc ccattaggct
    30901 gtggcgcgct ggcgggaacg gcctatgaaa tcgaccgtga acagttagca ggctggctgg
    30961 gctttgcttc ggcgacccgt aacagtctgg acagcgtttc tgaccgtgac catgtgttgg
    31021 aactgctgtc ggctgccgct atcggcatgg tgcatctgtc gcgttttgct gaagatctga
    31081 ttttctttaa caccggcgaa gcggggtttg tggagctttc tgaccgcgtg acttccggtt
    31141 catcattaat gccgcagaag aaaaacccgg atgcgctgga gctgattcgc ggtaaatgcg
    31201 gtcgggtgca gggtgcgtta accggcatga tgatgacgct gaaaggtttg ccgctggctt
    31261 acaacaaaga tatgcaggaa gacaaagaag gtctgttcga cgcgctcgat acctggctgg
    31321 actgcctgca tatggcggcg ctggtgctgg acggcattca ggtgaaacgt ccacgttgcc
    31381 aggaagcggc tcagcagggt tacgccaacg ccaccgaact ggcggattac ctggtggcga
    31441 aaggcgtacc tttccgcgag gcgcaccata tcgtgggtga agcggtggtg gaagccattc
    31501 gtcagggcaa accgctggaa gatctgccgc tcgacgagtt gcagaaattc agcccggtga
    31561 ttggcgaaga tgtctatccg attttgtcgc tgcaatcgtg cctcgacaag cgtgcggcaa
    31621 aaggcggcgt ctcaccgcag caggtggccc aagcgattgc ttttgcgcag gcgcggttgg
    31681 ggtaagaaca tttatatgta caaatttgag cctggcttat cgccgggctt ttttatggca
    31741 aaaaaaagcg gatcctggag atccgcaaaa gttcacgttg gctttagtta ttcgagttga
    31801 gaaactctcg aaacgggcag tgacttcaag ggttaaaaga ggtgccgctc cgtttctgtg
    31861 agcaattatc agtcagaatg cttgataggg ataatcgttc attgctattc tacctatcgc
    31921 catgaactat cgtggcgatg gaggatggat aatgaatatt cgtgatcttg agtacctggt
    31981 ggcattggct gaacaccgcc attttcggcg tgcggcagat tcctgccacg ttagccagcc
    32041 gacgcttagc gggcaaattc gtaagctgga agatgagctg ggcgtgatgt tgctggagcg
    32101 gaccagccgt aaagtgttgt tcacccaggc gggaatgctg ctggtggatc aggcgcgtac
    32161 cgtgctgcgt gaggtgaaag tccttaaaga gatggcaagc cagcagggcg agacgatgtc
    32221 cggaccgctg catattggtt tgattcccac ggttggaccg tacctgctac cgcatattat
    32281 cccgatgctg caccagacct ttccaaagct ggaaatgtat ctgcatgaag cacagaccca
    32341 ccagttactg gcgcaactgg acagtggcaa actcgattgc gtgatcctcg cgctggtgaa
    32401 agagagcgaa gcattcattg aagtgccgtt gtttgatgag ccaatgttgc tggctatcta
    32461 tgaagatcac ccgtgggcga accgcgaatg cgtaccgatg gccgatctgg caggggaaaa
    32521 actgctgatg ctggaagatg gtcactgttt gcgcgatcag gcaatgggtt tctgttttga
    32581 agccggggcg gatgaagata cacacttccg cgcgaccagc ctggagacgc tgcgcaacat
    32641 ggtggcggca ggtagcggga tcactttact gccagcgctg gctgtgccgc cggagcgcaa
    32701 acgcgatggg gttgtttatc tgccgtgcat taagccggaa ccacgccgca ctattggcct
    32761 ggtttatcgt cctggctcac cgctgcgcag ccgctatgag cagctggcag aggccatccg
    32821 agcaagaatg gatggctatt tcgataaagt gttaaaacag gcggtttaaa ccgtttaacg
    32881 cagctacccg ataggcttcc gccatggtcg ggtagttaaa ggtggtgttg acgaagtact
    32941 caatagtgtt gccgccacct ttctgttcca taatcgcctg accgatatga ataatttcgg
    33001 cagcgcgctc gccaaagcag tgaataccca gaatctcttt tgtttcccga tggaacaaaa
    33061 ttttcagcgt gcccacgttc atgccgacga tttgtgcgcg tgccagatgt ttaaactggg
    33121 cgcggcccac ttcatatggc actttcatcg cggtcagctg ctgttcggtt ttgcccacag
    33181 agctgatttc cgggatggtg taaatgccgg tagggatatc ttcaatcaga tgcgcggtgg
    33241 cttcgccttt taccagcgcc tgcgcggcaa tgcgcccctg gtcataggcc gctgatgcca
    33301 ggctcggata accaatcacg tcgcccaccg cgtaaacgtg cggctgtgcg gtctgataca
    33361 tgctgttgac cttcagctgt ccgcgactgt cagtttccag cccaatgttc tgtaacgcca
    33421 gcgaatcggt attaccggta cgaccgttgg catagagcag gcagtcagct ttcagttttt
    33481 tacccgactt cagatgcatg atcacaccgt cgtcacagcc ttcgatcttc tcgtactctt
    33541 cgttgtgacg aatcactacg ccactgttcc agaagtgata ggagagagaa tctgacatct
    33601 cttgatcgag aaatgccagc aggcgatcgc gggtgttgat cagatccact tttacatcca
    33661 taccgcggaa gatcgacgca tattcacagc cgatcactcc agcaccatag ataagtacat
    33721 ggcgcggttc gtggtgcatg ctaagaattg agtcgctgtc gtaaatgcgt ggatgggtga
    33781 aatcaacatc tgttggatga tatggacgag agccgcaggc aataacaaat ttttcagcgg
    33841 ttagtgtttc gacgctgccg tccgggcaat ccagcgccaa cgtatgctcg tcaacaaagc
    33901 gagcgttccc ctgcaatatt tcacagtgat tacgttcgta aaatccctga cgcatgcgcg
    33961 tttgttgatt aatcacgtta tcggcatggt taaggatatc ggcaaaagaa gagcggagca
    34021 gtcgcgaatg gtcgctgtat agtgggtttt gattgaattc tataatgcgg ctgacggcgt
    34081 gacggagagc tttcgacggg atggtgcccc agtgggtgca accgccgcca acattttgat
    34141 aacgctcgat aactgcgacg cgcacacctt gcttaaccag gcccattgca gcgccttcgc
    34201 cgccggggcc ggaacctatt actatggcat cgtaatcgta ggaatgtggc atggtagggc
    34261 ttacctgttc ttatacataa aagcaacaga atggtaacat tctatcgcgg gtaagccaat
    34321 tgatccccgt catttatctg gctatatcct gagcggcctt tgctttgtct gtttcttact
    34381 tttgccctga cgttttattg gatttttatc gacgatactc tccgtttaag cggcaggttt
    34441 ccgctgtacg taaaagaacc ggccagtaaa tatgttttat tgcgttaccg ttcattcaca
    34501 atactggagc aatccagtat gttcattctc tggtatagtg ccagcagtac ttttggcaag
    34561 gattcagaca tcgtgatggg cataagagcg caacaaaaag aaaaaacccg ccgttcgctg
    34621 gtggaagccg catttagcca attaagtgct gaacgcagct tcgccagcct gagtttgcgt
    34681 gaagtggcgc gtgaagcggg cattgctccc acctcttttt atcggcattt ccgcgacgta
    34741 gacgaactgg gtctgaccat ggttgatgag agcggtttaa tgctacgcca actcatgcgc
    34801 caggcgcgtc agcgtatcgc caaaggcggg agtgtgatcc gcacctcggt ctccacattt
    34861 atggagttca tcggtaataa tcctaacgcc ttccggttat tattgcggga acgctccggc
    34921 acctccgctg cgtttcgtgc cgccgttgcg cgtgaaattc agcacttcat tgcggaactt
    34981 gcggactatc tggaactcga aaaccatatg ccgcgtgcgt ttactgaagc gcaagccgaa
    35041 gcaatggtga caattgtctt cagtgcgggt gccgaggcgt tggacgtcgg cgtcgaacaa
    35101 cgtcggcaat tagaagagcg actggtactg caactgcgaa tgatttcgaa aggggcttat
    35161 tactggtatc gccgtgaaca agagaaaacc gcaattattc cgggaaatgt gaaggacgag
    35221 taatgaaaca agcaaatcaa gatagaggta cgctgctgct ggcgttagtt gctggcttat
    35281 cgattaatgg tactttcgca gcgctgttta gctccattgt gccattttct gtattcccga
    35341 ttatttccct ggtgctgacg gtttactgcc tgcatcaacg ttatcttaat cgcactatgc
    35401 cggtaggctt gccgggtctg gcagctgcct gttttattct cggcgtactg ctgtacagca
    35461 cggtagttcg tgcggaatat ccggatatcg gctctaactt cttcccggca gtactctccg
    35521 tcattatggt gttctggatt ggcgcgaaga tgcgtaaccg taagcaggaa gttgctgagt
    35581 aatcggtatt atgccggatg ctggcgcatc cggcatgggt tttacttcgc ggtcagtaat
    35641 acgccgcact ccatatggtg cgtgtagggg aactgatcaa acagagccag acgttcgacc
    35701 ttgtgcgtct ggcttaatgt ttccagattc ttgcataacg tttccgggtt acaggagatg
    35761 tacaaaatac acggatacgc ctgcaccatt ttctcggttt cactgtccag accgctgcgc
    35821 ggtgggtcga caaaaatggt ttcgcactga taactcttta agtcgatccc ttgcaggcgg
    35881 ttaaactcgc gcacaccatt catcgcctga gtaaattctt ctgccgccat acgaataatt
    35941 tgtacgttat caatatggtt agctgcgatg ttgtattgcg cagcagcaac cgacggctta
    36001 gcgatttcgg tggctaatac ccgatcaaaa ttgcgcgcca gcgctaatga aaagttaccg
    36061 ttgccgcagt acagctccag taaatcgcct tttgagcctt tggttacgtc cagcgcccat
    36121 tccagcatct gaatattcat cgccgcgttc ggctgggtaa agctgttttc tacctgacgg
    36181 tagatcatct ctttccctgc gaccggcaga cgctcgtcga tgtaatcttg atccagctcg
    36241 attttggttt tcgttgcccg accaatcaga tgcacattca gattctgcgc gcgcagtgca
    36301 tcgcgcaggg cctccgcttc ctgacgccac tcatcatcca gcttcttatg gtatagcagg
    36361 gaaaccaccg cctgattact cagcgtggtg aggtaatcaa tctggaacaa cttgtggcgc
    36421 agaacgggat tattacgcac acccgcaatc atcgccgtca tcaactggtt gataagttca
    36481 ctggcggcgg ggaagctatc cacgcggata cggcttttgg tttgttgatc gaaaatgatg
    36541 tgatacaggt catcgccatc gtgccagatg cggaactccg cacgcatccg gtaatgactg
    36601 accggcgagc gaaacacttc cggaaccagg tcagaaaacg gtgccatcat actttgcaaa
    36661 cgtaccactt tttcggctaa ctgcgcttca tactgttctg ttggaaggtg ttcgggggtc
    36721 atgatgtatc ctgaaaaatt aaagtacgcg gcgattgtag ggattgctca tcagatgtcc
    36781 agatcttgat gaattcctat ttgtgagcta cgtctggaca gtaacttgtt acaacctgta
    36841 gcatccactt gtcggtcctg tgagttaata gggaatccag tgcgaatctg gagctgacgc
    36901 gcagcggtaa ggaaaggtgc gatgattgcg ttatgcggac actgccatcc ggtgggaagt
    36961 catcatctct tagtatctta gatacccctc caagcccgaa gacctgccgg ccaacgtcgc
    37021 atctggttct catcatcgcg taatattgat gaaacctgcg gcatccttct tctattgtgg
    37081 atgctttaca atgattaaaa aagcttcgct gctgacggcg tgttctgtca cagccttttc
    37141 cgcttgggca caggatacca gcccggatac tctcgtcgtt actgctaacc gttttgaaca
    37201 gccgcgcagc actgtgcttg caccaaccac cgttgtgacc cgtcaggata tcgaccgctg
    37261 gcagtcgacc tcggtcaatg atgtgctgcg ccgtcttccg ggcgtcgata tcacccaaaa
    37321 cggcggttca ggtcagctct catctatttt tattcgcggt acaaatgcca gtcatgtgtt
    37381 ggtgttaatt gatggcgtac gcctgaatct ggcgggggtg agtggttctg ccgaccttag
    37441 ccagttccct attgcgcttg tccagcgtgt tgaatatatc cgtgggccgc gctccgctgt
    37501 ttatggttcc gatgcaatag gcggggtggt gaatatcatc acgacgcgcg atgaacccgg
    37561 aacggaaatt tcagcagggt ggggaagcaa tagttatcag aactatgatg tctctacgca
    37621 gcaacaactg ggggataaga cacggggaac gctgttgggc gattatgccc atactcatgg
    37681 ttatgatgtt gttgcctatg gtaatacagg aacgcaagcg cagacagata acgatggttt
    37741 tttaagtaaa acgctttatg gcgcgctgga gcataacttt actgatgcct ggagcggctt
    37801 tgtgcgcggc tatggctatg ataaccgtac caattatgac gcgtattatt ctcccggttc
    37861 accgttgctc gatacccgta aactctatag ccaaagttgg gacgccgggc tgcgctataa
    37921 cggcgaactg attaaatcac aactcattac cagctatagc catagcaaag attacaacta
    37981 cgatccccat tatggtcgtt atgattcgtc ggcgacgctc gatgagatga agcaatacac
    38041 cgtccagtgg gcaaacaatg tcatcgttgg tcacggtagt attggtgcgg gtgtcgactg
    38101 gcagaaacag actacgacgc cgggtacagg ttatgttgag gatggatatg atcaacgtaa
    38161 taccggcatc tatctgaccg ggctgcaaca agtcggcgat tttacctttg aaggcgcagc
    38221 acgcagtgac gataactcac agtttggtcg tcatggaacc tggcaaacca gcgccggttg
    38281 ggaattcatc gaaggttatc gcttcattgc ttcctacggg acatcttata aggcaccaaa
    38341 tctggggcaa ctgtatggct tctacggaaa tccgaatctg gacccggaga aaagcaaaca
    38401 gtgggaaggc gcgtttgaag gcttaaccgc tggggtgaac tggcgtattt ccggatatcg
    38461 taacgatgtc agtgacttga tcgattatga tgatcacacc ctgaaatatt acaacgaagg
    38521 gaaagcgcgg attaagggcg tcgaggcgac cgccaatttt gataccggac cactgacgca
    38581 tactgtgagt tatgattatg tcgatgcgcg caatgcgatt accgacacgc cgttgttacg
    38641 ccgtgctaaa cagcaggtga aataccagct cgactggcag ttgtatgact tcgactgggg
    38701 tattacttat cagtatttag gcactcgcta tgataaggat tactcatctt atccttatca
    38761 aaccgttaaa atgggcggtg tgagcttgtg ggatcttgcg gttgcgtatc cggtcacctc
    38821 tcacctgaca gttcgtggta aaatagccaa cctgttcgac aaagattatg agacagtcta
    38881 tggctaccaa actgcaggac gggaatacac cttgtctggc agctacacct tctgaaccac
    38941 gtcccaccgt gctggtgttt gactccggcg tcggtgggtt gtcggtctat gacgagatcc
    39001 ggcatctctt accggatctc cattacattt atgctttcga taacgtcgct ttcccgtatg
    39061 gcgaaaaaag cgaagcgttt attgttgagc gagtggtggc aattgtcacc gcggtgcaag
    39121 aacgttatcc ccttgcgctg gctgtggtcg cttgcaacac tgccagtacc gtttcacttc
    39181 ctgcattacg cgaaaagttc gacttcccgg ttgttggtgt cgtgccggcg attaaacctg
    39241 ctgcacgtct gacggcaaat ggcattgtcg gattactggc aacccgcgga acagttaaac
    39301 gttcttatac tcatgagctg atcgcgcgtt tcgctaatga atgccagata gaaatgctgg
    39361 gctcggcaga gatggttgag ttggctgaag cgaagctaca tggcgaagat gtttctctgg
    39421 atgcactaaa acgtatccta cgcccgtggt taagaatgaa agagccgcca gataccgttg
    39481 tattgggttg cacccatttc cctctactac aagaagaact gttacaagtg ctgccagagg
    39541 gaacccggct ggtggattct ggcgcagcga ttgctcgccg aacggcctgg ttgttagaac
    39601 atgaagcccc ggatgcaaaa tctgccgatg cgaatattgc cttttgtatg gcaatgacgc
    39661 cagaagctga acaattattg cccgttttac agcgttacgg cttcgaaacg ctcgaaaaac
    39721 tggcagtttt aggctgattt ggttgaatgt tgcgcggtca gaaaattatt ttaaatttcc
    39781 tcttgtcagg ccggaataac tccctataat gcgccaccac tgacacggaa caacggcaaa
    39841 cacgccgccg ggtcagcggg gttctcctga gaatctcaac agagaaaagc aaaaaaatgc
    39901 ttgactctgt
//
LOCUS       DADMKF010000020        39165 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.020, whole genome shotgun sequence.
ACCESSION   DADMKF010000020 DADMKF010000000
VERSION     DADMKF010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39165
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.020"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(<1..1112)
                     /gene="setA"
                     /locus_tag="JNC24_000956"
     CDS             complement(<1..1112)
                     /gene="setA"
                     /locus_tag="JNC24_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetA"
                     /protein_id="HAY9157773.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNAIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNALLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPL
                     AFMLALNYGFTVMFSIAAGIFTLSLVLIAFMLPSVARVELPSENALSMQGGWQDSNVR
                     MLFVASTLMWTCNTMYIIDMPLWISSELGLPDKLAGFLMGTAAGLEIPAMILAGYYVK
                     RYGKRRMMVIAVAAGVLFYTGLIFFHSRMALMTLQLFNAVFIGIVAGIGMLWFQDLMP
                     GRAGAATTLFTNSISTGVILAGVIQGAIAQSWGHFAV"
     gene            complement(1214..1345)
                     /gene="sgrT"
                     /locus_tag="JNC24_000957"
     CDS             complement(1214..1345)
                     /gene="sgrT"
                     /locus_tag="JNC24_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HAY9157774.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEGLMQWDDRNSES"
     gene            1434..3089
                     /locus_tag="JNC24_000958"
     CDS             1434..3089
                     /locus_tag="JNC24_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator SgrR"
                     /protein_id="HAY9157775.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIA
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPREWETLSNFASHPIGTGPYAVIRN
                     TTNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEEKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      3123..3222
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            3253..4236
                     /gene="thiB"
                     /locus_tag="JNC24_000959"
     CDS             3253..4236
                     /gene="thiB"
                     /locus_tag="JNC24_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HAY9157776.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFTFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVYPAFQNAIP
                     TGNWMYPVANVTQPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            4212..5822
                     /gene="thiP"
                     /locus_tag="JNC24_000960"
     CDS             4212..5822
                     /gene="thiP"
                     /locus_tag="JNC24_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HAY9157777.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALIQMMCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQSVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDISARYSMLCQSLGIEGWSRL
                     NVVELRALKRPLVQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            5806..6504
                     /gene="thiQ"
                     /locus_tag="JNC24_000961"
     CDS             5806..6504
                     /gene="thiQ"
                     /locus_tag="JNC24_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HAY9157778.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDSVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTDELL
                     SGKASASALLGITG"
     gene            complement(6574..7338)
                     /locus_tag="JNC24_000962"
     CDS             complement(6574..7338)
                     /locus_tag="JNC24_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HAY9157779.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWMAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(7424..8302)
                     /gene="araC"
                     /locus_tag="JNC24_000963"
     CDS             complement(7424..8302)
                     /gene="araC"
                     /locus_tag="JNC24_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HAY9157780.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRA
                     YWHEWLNWPPIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            8641..10341
                     /gene="araB"
                     /locus_tag="JNC24_000964"
     CDS             8641..10341
                     /gene="araB"
                     /locus_tag="JNC24_000964"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HAY9157781.1"
                     /translation="MAIAIGLDFGSDSVRALAVECATGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKIVLAELSAEQRAAVVGIGVDTTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKAQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATNAPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVATL
                     "
     gene            10352..11854
                     /gene="araA"
                     /locus_tag="JNC24_000965"
     CDS             10352..11854
                     /gene="araA"
                     /locus_tag="JNC24_000965"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HAY9157782.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLAVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            12023..12718
                     /gene="araD"
                     /locus_tag="JNC24_000966"
     CDS             12023..12718
                     /gene="araD"
                     /locus_tag="JNC24_000966"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAY9157783.1"
                     /translation="MLEDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQSGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            12793..15144
                     /gene="polB"
                     /locus_tag="JNC24_000967"
     CDS             12793..15144
                     /gene="polB"
                     /locus_tag="JNC24_000967"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HAY9157784.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IPADQVPRAQHILQGKQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASSLDFELEYVASRPQLLEKLNAWFANYDPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHTSPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDVIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRETIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            15308..18214
                     /gene="rapA"
                     /locus_tag="JNC24_000968"
     CDS             15308..18214
                     /gene="rapA"
                     /locus_tag="JNC24_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HAY9157785.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPVQTEGFDDLIKNCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRMEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            18226..18885
                     /gene="rluA"
                     /locus_tag="JNC24_000969"
     CDS             18226..18885
                     /gene="rluA"
                     /locus_tag="JNC24_000969"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HAY9157786.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(19002..19817)
                     /gene="djlA"
                     /locus_tag="JNC24_000970"
     CDS             complement(19002..19817)
                     /gene="djlA"
                     /locus_tag="JNC24_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HAY9157787.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            20072..22426
                     /gene="lptD"
                     /locus_tag="JNC24_000971"
     CDS             20072..22426
                     /gene="lptD"
                     /locus_tag="JNC24_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HAY9157788.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWQHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKLLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYRYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            22479..23765
                     /gene="surA"
                     /locus_tag="JNC24_000972"
     CDS             22479..23765
                     /gene="surA"
                     /locus_tag="JNC24_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HAY9157789.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            23765..24754
                     /gene="pdxA"
                     /locus_tag="JNC24_000973"
     CDS             23765..24754
                     /gene="pdxA"
                     /locus_tag="JNC24_000973"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HAY9157790.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTAGTLTLLPVALRESVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDAGIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLNELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            24751..25572
                     /gene="rsmA"
                     /locus_tag="JNC24_000974"
     CDS             24751..25572
                     /gene="rsmA"
                     /locus_tag="JNC24_000974"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HAY9157791.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            25575..25952
                     /gene="apaG"
                     /locus_tag="JNC24_000975"
     CDS             25575..25952
                     /gene="apaG"
                     /locus_tag="JNC24_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HAY9157792.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            25959..26801
                     /gene="apaH"
                     /locus_tag="JNC24_000976"
     CDS             25959..26801
                     /gene="apaH"
                     /locus_tag="JNC24_000976"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase
                     (symmetrical)"
                     /protein_id="HAY9157793.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGTLTCLRWEDKQYFVQPSNRHKDLGE
                     AAAS"
     gene            complement(26879..27358)
                     /gene="folA"
                     /locus_tag="JNC24_000977"
     CDS             complement(26879..27358)
                     /gene="folA"
                     /locus_tag="JNC24_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HAY9157794.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(27550..29412)
                     /gene="kefC"
                     /locus_tag="JNC24_000978"
     CDS             complement(27550..29412)
                     /gene="kefC"
                     /locus_tag="JNC24_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HAY9157795.1"
                     /translation="MDSHTLIQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLHWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLATSSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLLENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGTAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(29405..29935)
                     /gene="kefF"
                     /locus_tag="JNC24_000979"
     CDS             complement(29405..29935)
                     /gene="kefF"
                     /locus_tag="JNC24_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HAY9157796.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVLSHGWAYGHGGKALHGKYLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(30043..31374)
                     /locus_tag="JNC24_000980"
                     /pseudo
     CDS             complement(30043..31374)
                     /locus_tag="JNC24_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414587.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(31433..31720)
                     /gene="fixX"
                     /locus_tag="JNC24_000981"
     CDS             complement(31433..31720)
                     /gene="fixX"
                     /locus_tag="JNC24_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HAY9157797.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQALELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(31717..33003)
                     /locus_tag="JNC24_000982"
     CDS             complement(31717..33003)
                     /locus_tag="JNC24_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAY9157798.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFAESAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVILAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGYPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(33054..33995)
                     /locus_tag="JNC24_000983"
     CDS             complement(33054..33995)
                     /locus_tag="JNC24_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HAY9157799.1"
                     /translation="MNTFSQVWVFSDTPSRLPELMNGAQALANQINTFVLNDADGAQA
                     IQLGANHVWKLNGKPDDRMIEDYAGVMADTIRQHGADGLVLLPNTRRGKLLAAKLGYR
                     LKAAVSNDASTVSVQDGKATVKHMVYGGLAIGEERIATPYAVLTISSGTFDAAQPDAS
                     RTGETHTVEWQAPAVAITRTATQARQSNSVDLDKARLVVSVGRGIGSKENIALAEQLC
                     KAIGAELACSRPVAENEKWMEHERYVGISNLMLKPELYLAVGISGQIQHMVGANASQT
                     IFAINKDKNAPIFQYADYGIVGDAVKILPALTAALAR"
     gene            complement(34010..34780)
                     /locus_tag="JNC24_000984"
     CDS             complement(34010..34780)
                     /locus_tag="JNC24_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HAY9157800.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAEAA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
     gene            35253..36767
                     /locus_tag="JNC24_000985"
     CDS             35253..36767
                     /locus_tag="JNC24_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiporter"
                     /protein_id="HAY9157801.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVLGLTASTWILWTVLGSNTLLLIDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            36798..37940
                     /gene="caiA"
                     /locus_tag="JNC24_000986"
     CDS             36798..37940
                     /gene="caiA"
                     /locus_tag="JNC24_000986"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HAY9157802.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            38069..39165
                     /gene="caiB"
                     /locus_tag="JNC24_000987"
                     /pseudo
     CDS             38069..39165
                     /gene="caiB"
                     /locus_tag="JNC24_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
BASE COUNT         9364 a        10766 c        10257 g         8778 t
ORIGIN      
        1 acagcaaagt gcccccaact ttgtgcaatt gctccctgaa taacgccagc cagaattacc
       61 ccggtagaaa tactgttagt aaataaggtg gtagctgccc ccgctcttcc aggcattaaa
      121 tcctgaaacc atagcatccc aatacccgca acaatgccga taaatacagc gttaaaaagt
      181 tgcagcgtca tcaacgccat acggctatga aagaaaatca atccggtgta aaacagtact
      241 cctgccgcca ctgctatgac catcattcgc cgcttaccat aacgtttgac atagtagcca
      301 gccagaatca ttgctggtat ttccagtcca gctgccgtcc ccatcaggaa acccgccagt
      361 ttgtctggca atcctaactc gctactgatc cacaacggca tatcaataat gtacatggtg
      421 ttgcaggtcc acattaacgt cgaggcgaca aataacatcc gtacgttact atcctgccag
      481 ccaccttgca ttgataaagc attttccgac ggcagttcta cccgcgccac agacggaagc
      541 ataaatgcaa tcaataccag actgagtgtg aatatcccgg cggcaatcga aaacatcacc
      601 gtaaagccgt aattcaacgc cagcataaag gccaacggtg gaccgataac ccatgccaga
      661 gaaagctgcg cacgcatcac cgagctaaac atcaccactt ctcgcgccga gttatccgca
      721 tattcccgcg ccagagcaaa taactgtggc attgccgtat tggccagaga tgccagaagc
      781 acaccacagg tgataagcgt cagataatga cgattaaatg caaacaatag cgcattgccg
      841 atagccatca aacagcaaaa tataatcagt tttcgccgat cgccctgact gtcagaacgt
      901 tttgccaacc agaggcttac gccgatccca gcaatagcat tcaccgtata aaagagtccg
      961 atccagaaag gttgcgcgcc aacctcacga ctcagaaata agctcaatgt aggagcctgt
     1021 agcgccccgg ccacccccat cataaaagcg accagcataa atgccgcgta aacaccgttc
     1081 atacggcgag ccatcgtcat tatccagatc atacgttccc tttttagcgc ggcgagaata
     1141 aaaaaaacca gcaggtataa tctgctggcg ggtgatttta caccaatact cagtcacaca
     1201 tgatgcaggc aagtcaactt tcagaattgc ggtcatccca ctgcatcagt ccttcaagca
     1261 tggttaatcg ttgtgggacg cttaaccaac gcaaccagca caacttcgct gtcgcggtaa
     1321 aatagtgctg ataaaactga cgcatggggc acctccttgc ttcatcgtta ggaattattg
     1381 acttaatata gggaaaataa aattgctgtc ttttgcgcag gagttcccct tttatgccat
     1441 ctgctcgtct gcaacaacag ttcatccgcc tgtggcaatg ctgcgagggt aaatcgcagg
     1501 acacaacgct caacgaactg gcagcgttat tgagctgctc gcgtcgtcat atgcgtaccc
     1561 tgctcaacac catgcaggat cgcggctggc tgacgtggga agcggaagtc gggcgcggta
     1621 aacgctcgcg tctgacattc ctctataccg ggctggcgct tcagcaacag cgggcggaag
     1681 acctgctgga gcaggatcgt atcgaccaac tagtgcagtt ggttggcgac aaagcgactg
     1741 tgcggcaaat gctggtttct catctgggcc gcagcttccg ccaggggcgg cacatcctgc
     1801 gcgtgctcta ctatcgtccg ttgcgtaatc tgctacctgg cagcgcattg cgccgttccg
     1861 aaacccatat tgcccggcaa atcttcagtt ccctaacgcg cataaatgag gaaaatgggg
     1921 aactggaagc agacatcgcc caccactggc agcaaatatc accgcttcac tggcgtttct
     1981 ttttgcgtcc aggagtccat tttcatcatg gtcgtgaact ggaaatggac gacgtgatcg
     2041 cctctttaaa acgaatcaat acgctgccgc tctattcgca tattgcggac attgtgtcgc
     2101 cgacgccctg gacgctggat atccatctca cgcaaccgga ccgctggtta ccgttactgc
     2161 tggggcaagt tccggcgatg atcctgccgc gcgaatggga aaccctcagt aactttgcca
     2221 gccatcccat cggcaccggt ccgtatgcgg tgatccgcaa caccaccaat caactgaaaa
     2281 ttcaggcatt cgatgacttc ttcggttacc gggcattaat cgatgaagtt aacgtctggg
     2341 ttctgccgga aattgccgac gagccagctg gaggtctgat gctaaaaggt ccacagggtg
     2401 aggaaaaaga gattgaaagc cgcctggagg aaggttgcta ctatttacta ttcgacagcc
     2461 gcacccatcg cggggcgaat cagcaagtca gggactgggt aagctatgtg ctttctccaa
     2521 ctaatctggt ctatttcgct gaggaacagt accagcaact gtggttcccg gcttatggac
     2581 tgctcccccg ttggcaccat gcccgcacca taaagagcga aaaaccggct ggcctggaaa
     2641 gcctcaccct gaccttttat caggatcaca gtgagcatcg ggtgattgcc gggatcatgc
     2701 agcagattct ggcaagtcac caggtcacgc tggaaatcaa agagatcagc tacgatcagt
     2761 ggcatgaagg agagatcgag agcgatatct ggcttaacag cgccaacttt acgctgccgc
     2821 tggatttttc gctgttcgcg cacttgtgcg aggtgccgct gctccaacac tgtattccga
     2881 tcgactggca agccgacgcc gcccgctggc gcaatggcga aatgaacctg gcgaactggt
     2941 gccagcaact ggtcgccagc aaagcaatgg tgccgcttat ccaccactgg ctgatcattc
     3001 aggggcaacg cagtatgcgc ggcctgcgca tgaacaccct cggctggttc gattttaaat
     3061 cagcgtggtt tgcgccaccg gatccatgat tgctggtaga taacaaaatc actacactaa
     3121 cgccgttctc aacggggtgc cacgcaaacg cgtgcgctga gaaaataccc gtcgaacctg
     3181 atccggataa cgccgacgaa gggatttgag gctccttctc aagtcctttg ccactctttt
     3241 ttgaggtgca aagtgttaaa aaaatgtctt cccctgctgt tgctgtgcac agcgcccgtt
     3301 ttcgctaaac ccgttctgac tgtttatacc tacgattcct tcgccgccga ctgggggcct
     3361 ggtccaaaga tcaaaaaagc ttttgaagcc gactgtaact gcgaactgaa actggtggcg
     3421 ctggaagatg gcgtttcgct tctcaaccgt ctacggatgg aaggcaaaaa tagtaaagcc
     3481 gatgtggtgc tggggctgga taacaatctg ttagacgccg ccagtaaaac cggactattt
     3541 gccaaaagcg gtgtggcagc ggatgccgtt aacgttcccg gcggctggaa taatgacact
     3601 ttcgtcccgt ttgattacgg ctatttcacc ttcgtctatg acaagaacaa actgaaaaac
     3661 ccgccgcaaa gcctgaaaga attggttgag agcgatcaga actggcgggt gatttatcag
     3721 gatccgcgta ccagcacgcc ggggctgggt ctgctgttgt ggatgcaaaa agtctatggc
     3781 gatgacgccc cacaagcctg gcagaaactg gcgaagaaaa ctgtcacggt caccaaaggc
     3841 tggagcgaag cctatggcct gtttttaaaa ggcgaaagcg atctggtact gagttacacc
     3901 acctctccgg cttatcacat tctcgaagag aagaaagata actacgccgc cgcgaacttc
     3961 agcgaaggtc actatctgca ggtggaagtc gccgcccgca ccgctgccag caagcagccg
     4021 gagctggcgc aaaaattcct ccagtttatg gtttatccgg cttttcagaa tgcgatccca
     4081 accggcaact ggatgtatcc ggtggcaaac gtcacgcagc ctgccggttt tgaacaattg
     4141 accaaaccag caaccacgct ggagttcacg ccagccgaag tggcggcaca acgtcaggca
     4201 tggattagcg aatggcaacg cgccgtcagc cgttaattcc cggctggtta attccaggtg
     4261 taagcgccgc cacgctggtg gtagcggttg cgctcgcggc gtttctcgcc ctgtggtgga
     4321 acgcgccgca gggtaactgg gtagcagtct ggcaggacag ctacctgtgg catgtggtgc
     4381 gcttctcctt ctggcaggcg tttctctcgg cgctactctc tgtcgtaccc gcgatattcc
     4441 tcgcccgcgc cctctatcgc aggcgctttc cgggtcggct ggcgctgttg cgtctgtgcg
     4501 caatgacctt gatcctcccg gtgctggttg ctgttttcgg cattcttagc gtctatggtc
     4561 gtcagggctg gctggcatcg ctctgccaat cgctcggtct ggagtggacc ttttcgccct
     4621 acggcctgca aggcattttg ctggcccatg tgttttttaa tctgccgatg gcgagccgtt
     4681 tattacttca ggcactggaa aacattcccg gcgaacaacg tcaacttgcc gcccagcttg
     4741 gaatgcgtgg ctggcacttc tttcgtttcg tcgaatggcc gtggttacgg cgacaaatcc
     4801 cgccagttgc tgcgcttatc tttatgctct gtttcgccag cttcgccacc gtactgtcgc
     4861 taggcggcgg tccgcaagcg accactatcg agctggctat ttatcaggcg ctgagttacg
     4921 actacgatcc tgcccgcgcg gcgatgctgg cgctgatcca gatgatgtgt tgccttgggc
     4981 tggtgctgtt gagtcagcga ttgagtaagg ccattgcgcc cggcaccacg ctgctgcaag
     5041 gctggcgcga cccggacgat cgtctgcata gccgcatttg cgacacggtg ttaatagtgc
     5101 tggcgctgct gctgttgttg ccaccgttgc tggcggtgat cgtcgatggg gtaaatcgcc
     5161 agttgccgga agtgctggca caatcggtgc tgtggcaggc gctgtggacc tcgttgcgta
     5221 ttgcgctggc ggcaggtgta ttgtgcgtag tgctgaccat gatgctgcta tggagcagtc
     5281 gcgaacttcg ggcgcggcag aaaatgctgg cgggtcaggc gctggagatg agcggcatgt
     5341 tgatcctcgc catgccgggg attgtgctgg ctaccggctt ctttttactg ctcaacaaca
     5401 ctatcggcct gccacaatct gctgacggca ttgtgatttt caccaatgcg ttaatggcga
     5461 tcccttacgc gctgaaagta ctggaaaacc cgatgcgcga tatctccgcc cgctacagca
     5521 tgttgtgtca gtcgttgggc attgaaggct ggtcacgctt aaacgtggtg gagctgcgcg
     5581 ccctgaaacg cccactggtg caggcgctgg cctttgcatg cgtgctgtcg attggtgatt
     5641 ttggcgtggt ggcgttgttc ggtaacgatg atttccgcac cctgccgttt tatctctacc
     5701 agcaaattgg ctcctatcgc agccaggacg gcgcggtcac cgcgttaatt ctgctactac
     5761 tctgttttct gctgtttacc gtgattgaaa aactaccggg gcgaaatgtt aaaactgact
     5821 gatatcacct ggctttacca ccatttgccg atgcgtttta gcttaacggt agaacgcggc
     5881 gagcaggtgg cgatcctcgg gccaagcggc gcaggtaaaa gtaccctgct gaatttgatt
     5941 gccggttttc tgacgccagc cagcggttcg ctgactatcg acagcgttga tcacaccacg
     6001 acgccgccgt cacgccgtcc ggtatcgatg ctgtttcagg agaacaacct gttcagccac
     6061 ctgacggtcg cgcagaacat cgggctgggg cttaatccgg gattgaagct gaacgcggcg
     6121 cagcaggaga aaatgcacgc tatcgcccgc cagatgggga ttgataattt aatggcgcgg
     6181 ttaccgggcg agctttccgg cggtcagcga cagcgcgtgg cgttagcgcg ttgtctggta
     6241 cgcgagcagc cgattttatt gctcgatgaa ccgttctctg cgctcgatcc ggcgctacgc
     6301 caagagatgt tgacgctggt gagcacgagc tgccagcagc aaaaaatgac gttattgatg
     6361 gtgtcgcaca gtgtggaaga tgccgcgcgg atcgccacgc gctcggtggt ggtcgccgac
     6421 gggcgcatcg cctggcaggg taaaaccgat gaattgttaa gcggtaaggc gagtgcttcg
     6481 gcgctgttgg gaattacagg ttaaaagcgc acggacagtc ccctcgcccc tctggggaga
     6541 agggaacaaa ccagcgccga tgcaaactat cccctaaacc ccaaccactt tacgcaaaat
     6601 atcgatatac accggcatca acgggtggcg aattaacacc accagcgcca ccacgccgat
     6661 ggcagaaatc aacggcgtca gccacaacaa acgaccgcgg gacaaataat gactcaaacg
     6721 gtcagtcgct ttaccactgc gccataaccg ccagcacagc cagccaccaa cccacaaaaa
     6781 taccgccgtc gccagcagca accatttaaa ctcaccgctc tgcatcccgg caggaatatc
     6841 gatcgccgcg cccgccagaa tccctggcag gaagtaaaac ggcggccaca gcaggcagcc
     6901 gataatattc ggcgtgataa attttgccac tggcagatcc agcattcccg ccaccattgg
     6961 caccagcgga cgcgtcgggc caacaaaacg accgaccaga atggtgaaca tgctgtgttg
     7021 atgcaacgca tgttcagttt tatcaagtag tgctttgttt ttcttcagaa atgaccagcg
     7081 atgcaacggc tttttaaaac gccagcccag ccagaaggag atccagtcgc ccagcaggca
     7141 accgacaatc cccgccatcc acgcgtgcca gaaacttaac tcgccgctgc caatcagcgc
     7201 tcccagcccc gccatcagca ccgtaccggg tagaatcaaa ccgaccagcg ccagcgactc
     7261 cagaaaggcc accaacacca ccgccatcaa tgaatacacg gtggattggg taataaagtg
     7321 ttccagcaat gcttgcataa tgtgcctgtc aaatggacga agcagggatt ctgcaaaccc
     7381 tatgctactc cgtcaagccg tcaattgtct gattcgttac caattatgac aacttgacgg
     7441 ctacatcatt cactttttct tcacaaccgg cacggaactc gctcgggctg gccccggtgc
     7501 attttttaaa tacccgcgag aaatagagtt gatcgtcaaa accaacattg cgaccgacgg
     7561 tggcgatagg catccgggta gtgctcaaaa gcagcttcgc ctgactaatg cgttggtcct
     7621 cgcgccagct taagacgcta atccctaact gctggcggaa aagatgtgac agacgcgacg
     7681 gcgacaagca aacatgctgt gcgacgctgg cgatatcaaa attgctgtct gccaggtgat
     7741 cgctgatgta ctgacaagcc tcgcgtacac gattatccat cggtggatgg agcgactcgt
     7801 taatcgcttc catgcgccgc agtaacaatt gctcaagcag atttatcgcc agcagctccg
     7861 aatagcgccc ttccccttgc ccggcgttaa tgatttgccc aaacaggtcg ctgaaatgcg
     7921 gctggtgcgc ttcatctggg cgaaagaaac ccgtattggc aaatattggc ggccagttaa
     7981 gccattcatg ccagtaggcg cgcggacgaa agtaaaccca ctggtgatac cattcgcgag
     8041 cctccggatg acgaccgtag tgatgaatct ctcctggcgg gaacagcaaa atatcacccg
     8101 gtcggcagac aaattctcgc ccctgatttt tcaccacccc ctgaccgcga atggtgagat
     8161 tgagaatata acctttcatt cccagcggtc ggtcgataaa aaaatcgaga taaccgttgg
     8221 cctcaatcgg cgttaaaccc gccaccagat gggcgttaaa cgagtatccc ggcagcaggg
     8281 gatcattttg cgcttcagcc atacttttca tactcccgcc attcagagaa gaaaccaatt
     8341 gtccatattg catcagacat tgccgtcact gcgtctttta ctggctcttc tcgctaaccc
     8401 aaccggtaac cccgcttatt aaaagcattc tgtaacaaag cgggaccaaa gccatgacaa
     8461 aaacgcgtaa caaaagtgtc tataatcacg gcagaaaagt ccacattgat tatttgcacg
     8521 gcgtcacact ttgctatgcc atagcatttt tatccataag attagcggat cctacctgac
     8581 gctttttttc gcaactctct actgtttctc catacccgtt tttttggatg gagtgaaacg
     8641 atggcgattg caattggcct cgattttggc agtgattctg tgcgagcttt ggcggtggaa
     8701 tgcgctaccg gtgaagagat cgccaccagc gtagagtggt atccccgttg gcagaaaggg
     8761 caattttgtg atgccccgaa taaccagttc cgccatcatc cgcgtgacta cattgagtca
     8821 atggaagcgg cgctgaaaat cgtgcttgca gagcttagcg ccgaacagcg cgcagctgtg
     8881 gtcgggattg gcgttgacac aaccggctcg acgcccgcac cgattgacgc cgacggtaac
     8941 gtgctggcgc tgcgcccaga gtttgccgaa aacccaaacg cgatgttcgt attgtggaaa
     9001 gaccacactg cggttgaaga agcggaagag attacccgtt tgtgccacgc gccgggcaac
     9061 gttgactact cccgctatat tggcggtatt tattccagcg aatggttctg ggcaaaaatc
     9121 ctgcatgtga ctcgccagga cagcgccgtg gcgcaatctg ccgcatcgtg gattgagctg
     9181 tgcgactggg tgccagctct gctttccggt accacccgcc cgcaggatat tcgtcgcgga
     9241 cgttgcagcg ccgggcataa atctctgtgg cacgaaagct ggggcggctt gccgccagcc
     9301 agtttctttg atgagctgga cccgatcctc aatcgccatt tgccttcccc gctgttcact
     9361 gacacctgga ctgccgatat tccggtgggc accttatgcc cggaatgggc gcagcgtctc
     9421 ggcctgcctg aaagcgtggt gatttccggc ggcgcgtttg actgccatat gggcgcagtt
     9481 ggcgcaggcg cacagcctaa cgcactggta aaagttatcg gtacttccac ctgcgacatt
     9541 ctgattgccg acaaacagag cgttggcgag cgggcagtta aaggtatttg cggtcaggtt
     9601 gatggcagcg tggtgcctgg atttatcggt ctggaagcag gccaatcggc gtttggtgat
     9661 atctacgcct ggtttggtcg cgtactcggc tggccgctgg aacagcttgc cgcccagcat
     9721 ccggaactga aagcgcaaat caacgccagc cagaaacaac tgcttccggc gctgaccgaa
     9781 gcatgggcca aaaatccgtc tctggatcac ctgccggtgg tgctcgactg gtttaacggt
     9841 cgtcgcacgc caaacgctaa ccaacgtctg aaaggggtga ttaccgatct taacctcgct
     9901 accaacgctc cgctgctgtt cggcggtttg attgctgcca ccgcctttgg cgcacgcgca
     9961 atcatggagt gctttaccga tcaggggatc gccgtcaata acgtgatggc gctgggcggc
    10021 atcgcgcgga aaaaccaggt cattatgcag gcctgctgcg acgtgctgaa tcgcccgctg
    10081 caaattgttg cctctgacca gtgctgtgcg ctcggtgcgg cgatttttgc tgccgtcgcc
    10141 gcgaaagtgc acgcagacat cccatcagcc cagcaaaaaa tggccagtgc ggtagagaaa
    10201 accctgcaac cgcgcagcga acaggcacaa cgctttgaac agctttatcg ccgctatcag
    10261 caatgggcga tgagcgccga acaacactat cttccaactt ccgccccggc acaggctgcc
    10321 caggccgttg cgactctata aggacacgat aatgacgatt tttgataatt atgaagtgtg
    10381 gtttgtcatt ggcagccagc atctgtatgg cccggaaacc ctgcgtcagg tcacccaaca
    10441 tgccgagcac gttgttaatg cgctgaatac ggaagcgaaa ctgccctgca aactggtgtt
    10501 gaaaccgctg ggcaccacgc cggatgaaat caccgctatt tgccgcgacg cgaattacga
    10561 cgatcgttgc gctggtctgg tggtgtggct gcacaccttc tccccggcca aaatgtggat
    10621 caacggcctg accatgctca acaaaccgtt gctgcaattc cacacccagt ttaacgcggc
    10681 gctgccgtgg gacagtatcg atatggactt tatgaacctg aaccagactg cacatggcgg
    10741 tcgcgagttc ggcttcattg gcgcgcgtat gcgtcagcaa catgccgtgg ttaccggtca
    10801 ctggcaggat aaacaagccc atgagcgtat cggctcctgg atgcgccagg cggtctctaa
    10861 acaggatacc cgtcatctga aagtctgccg ttttggcgat aacatgcgtg aagtagcggt
    10921 caccgatggt gataaagttg ccgcacagat caagttcggt ttctccgtca atacctgggc
    10981 ggttggcgat ctggtgcagg tggtgaactc catcagcgac ggcgatgtta acgcgctggt
    11041 cgatgagtac gaaagctgct acaccatgac gcctgccaca caaatccacg gcgaaaaacg
    11101 acagaacgtg ctggaagcgg cgcgtattga gctggggatg aagcgtttcc tggaacaagg
    11161 tggcttccac gcgttcacca ccacctttga agatttgcac ggtctgaaac agcttccagg
    11221 tctggccgta cagcgtctga tgcagcaggg ttacggcttt gcgggcgaag gcgactggaa
    11281 aaccgccgcc ctgcttcgca tcatgaaggt gatgtcaacc ggtctgcagg gcggcacctc
    11341 ctttatggag gactacacct atcacttcga gaaaggtaat gacctggtgc tcggctccca
    11401 tatgctggaa gtctgcccgt cgattgccgt agaagagaaa ccgatcctcg acgttcagca
    11461 tctcggtatt ggtggtaagg acgatcctgc ccgactgatc ttcaataccc aaaccggtcc
    11521 agcgattgtc gccagcctga ttgatctcgg cgatcgttac cgtctgctgg ttaactgtat
    11581 cgacacggtg aaaacaccgc actccctgcc gaaactgccg gtggcgaatg cgctgtggaa
    11641 agcgcaaccg gatctgccaa ctgcttccga agcgtggatc ctcgctggtg gcgcgcacca
    11701 taccgtcttc agccatgcgc tgaacctcaa cgatatgcgc cagttcgccg agatgcacga
    11761 cattgaaatc acggtgattg ataacgacac ccgcctgcca gcgtttaaag acgcactgcg
    11821 ctggaacgaa gtgtattacg gatttcgtcg ctaagtagcc acatcaggta tgtaatgcct
    11881 gatgcgacgc tgacgcgtct tatctggcct acacgctgcg attttgtagg ccggataagc
    11941 aaagcgcatc cggcattgtt ttccccctcg cccctttggg gagagggccg gggtgagggg
    12001 aaacgacctc tcaggagtca acatgttaga agatctcaaa cgccaggtat tagaagccaa
    12061 cctggcgctg ccaaaacaca acctggtcac gctcacatgg ggcaacgtca gcgccgttga
    12121 tcgcgagcgc ggcgtctttg tgatcaaacc ttccggcgtc gattacagcg tcatgaccgc
    12181 tgacgatatg gtcgtggtta gcatcgaaac cggtgaagtg gttgaaggta cgaaaaagcc
    12241 ctcctccgac acgccaactc accggctgct ctatcaggca ttcccctcca ttggcggcat
    12301 tgtgcatacg cactcgcgcc acgccaccat ctgggcgcag tcgggtcagt cgattccagc
    12361 aaccggcacc acccacgccg actatttcta cggcaccatt ccctgcaccc gcaaaatgac
    12421 cgacgcagaa atcaacggcg aatatgagtg ggaaaccggt aacgtcatcg tagaaacctt
    12481 tgaaaaacag ggtatcgatg cagcgcaaat gcccggcgtt ctggtccatt cccacggccc
    12541 gtttgcatgg ggcaaaaatg ccgaagatgc ggtgcataac gccatcgtgc tggaagaggt
    12601 cgcttatatg gggatattct gccgtcagtt agcgccgcag ttaccggata tgcagcaaac
    12661 gctgctggat aaacactatc tgcgtaagca tggcgcgaag gcatattacg ggcagtaatg
    12721 actgtataaa accacagcca atcaaacgaa accaggctat actcaagcct ggttttttga
    12781 tggattttca gcgtggcgca ggcaggtttt atcttaaccc gacactggcg ggacaccccg
    12841 caagggacag aagtctcctt ctggctggcg acggacaacg ggccgttgca ggttacgctt
    12901 gcaccgcaag agtccgtggc gtttattccc gccgatcagg ttccccgcgc tcagcatatt
    12961 ttgcagggta aacaaggctt tcgcctgaca ccgctggcgt taaaggattt tcaccgccag
    13021 ccggtgtatg gcctttactg tcgcgcccat cgccaattga tgaattacga aaagcgcctg
    13081 cgtgaaggtg gcgttaccgt ctacgaggcc gatgtgcgcc cgccagaacg ctatctgatg
    13141 gagcggttta tcacctcacc ggtgtgggtc gagggtgata tgcacaatgg cactatcgtt
    13201 aatgcccgtc tgaaaccgca tcccgactat cgtccgccgc tcaagtgggt ttctatagat
    13261 attgaaacca cccgccacgg tgagctgtac tgcatcggcc tggaaggctg cgggcagcgc
    13321 atcgtttata tgctggggcc ggagaatggc gacgcctcct cgcttgattt cgaactggaa
    13381 tacgtcgcca gccgcccgca gttgctggaa aaactcaacg cctggtttgc caactacgat
    13441 cctgatgtga tcatcggttg gaacgtggtg cagttcgatc tgcgaatgct gcaaaaacat
    13501 gccgagcgtt accgtcttcc gctgcgtctt gggcgcgata atagcgagct ggagtggcgc
    13561 gagcacggct ttaaaaacgg cgtctttttt gcccaggcta aaggtcggct aattatcgac
    13621 ggtatcgagg cgctgaaatc cgcgttctgg aatttctctt cattctcgct ggaaactgtc
    13681 gctcaggagc tattaggcga aggaaaatct atcgataacc cgtgggatcg aatggacgaa
    13741 attgaccgcc gtttcgccga agataaacct gcgctggcaa cttataacct gaaagattgc
    13801 gagctggtga cgcagatctt ccacaaaact gaaatcatgc catttttact cgaacgagca
    13861 acggtgaacg gcctgccggt ggatcggcac ggcggttcgg tggcagcgtt tggtcatctc
    13921 tattttccgc gaatgcatcg cgctggttat gtcgcgccta atctcggcga agtgccgccg
    13981 cacaccagcc ctggcggcta cgtgatggat tcacggccag ggctttatga ttcagtgctg
    14041 gtgctggact ataaaagcct gtacccgtcg atcatccgca cctttctgat tgatcccgtc
    14101 gggctggttg aaggcatggc gcagcctgat ccagagcaca gtaccgaagg ttttctcgat
    14161 gcctggttct cacgggagaa acattgcctg ccggagattg tgactaacat ctggcacggg
    14221 cgcgatgaag ccaaacgtca gggtaataaa ccactttcgc aggcgctgaa gatcattatg
    14281 aatgcctttt atggcgtgct tggcaccacc gcctgccgct ttttcgatcc gcgcctggca
    14341 tcgtcgatca ccatgcgtgg tcatcagatc atgcggcaaa ccaaagcatt gattgaagcg
    14401 cagggctacg acgttatcta cggagatacc gactcgactt ttgtctggct aaaaggtgca
    14461 cattcggaag aagaggcggc gaaaatcggt cgtgcactgg tgcagcacgt taacgcctgg
    14521 tgggcggaaa cgctgcaaaa acaacggctg accagcgcat tagaactgga gtatgaaact
    14581 catttctgcc gttttcttat gccaaccatt cgcggagctg ataccggcag taaaaagcgc
    14641 tacgccgggc tgattcagga gggcgataaa cagcggatgg tgtttaaagg gctggaaacc
    14701 gtgcgcaccg actggacgcc gctggcccag cagtttcagc aggagctata cctgcgcatc
    14761 ttccgcaacg agccatatca ggaatatgta cgcgaaacca tcgacaaact gatggcgggt
    14821 gaactggatg cgcgactggt ttaccgtaaa cgccttcgcc gtccgctgag cgagtatcag
    14881 cgtaatgtgc cgcctcatgt acgcgccgct cgccttgccg atgaagaaaa ccaaaagcgt
    14941 ggtcgcccct tgcaatatca gaatcgcggc accattaagt acgtatggac caccaacggc
    15001 ccggagccgc tggactacca acgttcacca ctggattacg aacactatct gacccgccag
    15061 ctacaacccg tggcggaggg aatactccct tttattgagg ataattttgc tacacttatg
    15121 accgggcaac ttgggctatt ttgagcaaaa aaagagttcg ccagatacca ttttgatgcg
    15181 tgacgaatgc tttgccatcc agtaccatag cgccctttcc attcctggac ctgaataaca
    15241 ccactacccc ataagcacgg tagcgggtgg ttattgcctg caattaaaga tatagagccg
    15301 aacacatatg ccttttacac ttggtcaacg ctggatcagc gatacagaaa gcgaattggg
    15361 acttggaacc gttgtcgcgg tggatgcgcg aactgtcact ttacttttcc catctactgg
    15421 tgaaaaccgt ctgtacgcac gcagtgattc ccccgtgacc cgcgtgatgt tcaaccctgg
    15481 tgatactatt accagccatg acggctggca gatgcaagtc gaagaagtaa aagaagaaaa
    15541 tggcttgctg acctatatcg gtactcgcct ggatactgaa gagtccggcg tagccctgcg
    15601 tgaagttttc cttgatagca aactggtgtt cagcaaaccg caggaccgtc tgtttgccgg
    15661 gcagattgac cgtatggacc gctttgcgct gcgttatcgc gcgcgtaagt attccagcga
    15721 acagttccgt atgccgtaca gcggcctgcg cggtcagcgt accagcctga tcccgcatca
    15781 gctcaacatc gctcatgatg ttggtcgccg ccacgcgccg cgcgtcctgc tggctgacga
    15841 agtgggttta gggaaaacca ttgaagccgg gatgatcctg catcagcaac tgctctctgg
    15901 cgctgctgaa cgtgtgctaa ttatcgtccc ggaaacctta cagcatcagt ggctggtaga
    15961 aatgctgcgc cgtttcaacc tgcgctttgc gctatttgat gatgagcgtt atgccgaagc
    16021 tcagcacgat gcttacaacc cgttcgacac cgaacagctg gtgatttgct cgctggattt
    16081 tgcccgtcgt agcaaacagc gcctggaaca tctctgtgaa gccgaatggg acctgctggt
    16141 ggtcgatgaa gcgcatcacc tggtgtggag cgaagatgcg ccaagccgtg aatatcaggc
    16201 cattgaacaa ctggcagagc acgtgccggg cgttctgctg ctgaccgcga ccccggaaca
    16261 gctggggatg gaaagccact tcgcccgtct gcgtctgctg gacccgaacc gtttccacga
    16321 ttttgcgcag ttcgttgaag agcagaaaaa ttatcgtccg gttgcggacg ccgttgccat
    16381 gctgctggca ggtaacaaac tgagcaatga cgaactgaac atgctcggcg agatgatcgg
    16441 cgagcaggat atcgagccgc tgttgcaggc agcaaacagc gacagcgaag atgcccagag
    16501 cgcccgtcag gagctggttt cgatgctgat ggatcgccac ggcaccagcc gcgtgctgtt
    16561 ccgtaacacg cgtaacggtg tgaaaggatt cccgaaacgc gagctgcaca ccattaagct
    16621 gccgctgccg acgcagtatc agacggctat taaagtctcc ggcattatgg gcgcacgtaa
    16681 aagtgcggaa gatcgtgctc gcgatatgct ctacccggag cgtatttatc aggaatttga
    16741 aggtgataac gccacctggt ggaacttcga tccgcgcgtt gagtggctga tgggctacct
    16801 gaccagccat cgctctcaga aagtgctggt gatctgcgcc aaagctgcca ctgcgctgca
    16861 actggagcag gtactgcgcg aacgtgaagg tattcgcgct gcggtgttcc acgaaggtat
    16921 gtcgattatc gaacgtgacc gcgctgccgc ctggtttgcc gaagaagaca ccggcgcaca
    16981 ggtactgctg tgctcagaaa tcggttctga aggacgtaac ttccagttcg ccagccacat
    17041 ggtgatgttt gacctgccat tcaacccgga tctactggag cagcgtattg gtcgtctgga
    17101 tcgtatcggc caggcgcacg atattcagat ccatgtgcct tatctggaga aaaccgctca
    17161 gtcggtgctg gtgcgctggt atcacgaagg tctggatgca tttgagcaca cctgcccgac
    17221 cggacgcact atttacgata gcgtatacaa cgatctgatt aactatctgg cttcaccggt
    17281 tcaaaccgaa ggctttgacg atctgatcaa aaactgccgc gagcaacatg aagcgctgaa
    17341 agcacagctg gaacagggtc gtgaccgcct gctggaaatc cactccaacg gtggcgaaaa
    17401 agcccaggca ctggcagaaa gcattgaaga gcaggatgac gataccaacc tgatcgcctt
    17461 cgccatgaac ctgttcgata ttatcggtat caatcaggac gatcgcggcg acaacatgat
    17521 cgtgctgacg ccgtccgatc atatgctggt gccggacttc cctggcctgt cggaagatgg
    17581 catcaccatc acctttgatc gtgaagtggc gctggcgcgt gaagatgcac agtttattac
    17641 ctgggagcat ccgctgatcc gcaacggtct ggatctgatc ctttctggcg atactggtag
    17701 cagcacgatt tcactattga aaaacaaagc gttgccggta ggtacgctgt tggtggaact
    17761 gatttatgtc gttgaagccc aggctccgaa gcagttgcag ctcaaccgct tcctgccacc
    17821 gacgccggta cgtatgctgc tggataaaaa cggcaacaac ctggcggcgc aggtagagtt
    17881 tgaaaccttt aaccgtcagc ttaacgcggt taaccgtcac accggcagca aactggttaa
    17941 cgccgtgcag caggatgttc acgctatcct tcagctgggt gaagcgcaga tcgagaaatc
    18001 tgcccgtgca ttgattgatg ccgcacgtaa cgaagccgac gaaaaactgt ctgccgagct
    18061 gtctcgtatg gaagcgctgc gcgcagtgaa cccgaacatt cgtgacgacg aactgaccgc
    18121 cattgagagc aaccgtcagc aggtaatgga aagcctggat caggcaggct ggcgtctgga
    18181 tgccctgcgt ttgatcgttg taacgcatca gtaacggagc cgaaaatggg gatggaaaac
    18241 tacaatccac cgcaggaacc ctggttggtt atcctgtatc aggatgacca cattatggtg
    18301 gttaacaagc cgagcggttt gttgtctgtg ccggggcgtc tggaagagca caaagacagc
    18361 gtgatgacgc gcattcagcg tgattatccg caggcagaat cggtgcatcg tctggatatg
    18421 gctaccagcg gcgtgattgt agtggcgctg accaaagccg cggagcggga gttaaaacgc
    18481 cagttccgcg agcgcgagcc gaaaaagcag tatgtagccc gcgtctgggg gcatccatcc
    18541 cctgcggaag gtctggtgga tctgccgctg atttgcgact ggccaaaccg cccgaaacag
    18601 aaagtctgtt acgaaacggg taaacctgcg cagacggaat atgaagtggt ggagtatgcg
    18661 gcggataaca cggcaagagt ggtgttaaaa ccgattaccg ggcgttcgca tcagctgcgt
    18721 gtgcatatgc tggcgctggg tcatccgatt ctcggcgatc gtttttatgc atcaccagaa
    18781 gcgagagcga tggcaccgcg tttgttgcta catgcagaga tgttgacgat tacccatccg
    18841 gcgtatggca atagtatgac gtttaaagcg ccagcggatt tttaatcgtc acgctggcgc
    18901 agttcggtag gcggataagg cgttcacgcc gcatccggca attgtgcgcc gattgcctga
    18961 tgcgacgcgg gccgcgtctt atcaggccta cagtttaaaa cttatttaaa ccctttctgc
    19021 tgctttatca gctcatatgc ctgctgaatt tcctgcgctt tctgcttcgc catctccatc
    19081 atctcaggcg gcaaaccttt cgccaccagc ttatcgggat ggtgttcact catcagctta
    19141 cggtaggcac gtttgatggt ggtcgcatca tccgtcggct tcacgcccag cacattacag
    19201 gcatcttcca gcgtcgggcc acgctgcgct tgctgccagt taccaccgcc agattgctgc
    19261 tgataaccgc cgccaaactg tgcaccgccc tgcatcatgc gcaaaaactg gtcaaactgg
    19321 gcgcgggaga tccctaattc ttcagcaatg acatacagca ccgcccgttc attcgggtgc
    19381 agtgaaccat cagcaaacgc cgcctgaatc tggatttcca gaaacatacg aattaagtca
    19441 aaacgaccaa agcagacact gcgaaactgg cgcatctttt cgcgcagcgg gtaattatct
    19501 gattttccca cccggaacgc gttttgcgcc gcagtacggg aagcgccatg aaggttcatt
    19561 cggtccatca actggctggc gatatgaata tcagcctccg tgacgcgacc tttggatttg
    19621 gttaaatgcc ccatcacttc aaaagtggtg gcaaaaaaca gcgcctgacg ctcacgctgg
    19681 ttggcgaacc acgccatttt acggctacgg gctttatcaa acatatggcc aattaacagg
    19741 cctaacacta cgccccaaaa gccgccgccc atcagtaagg ccacggccac gccaatgatt
    19801 tttccccaat actgcatata ttccccaaat cgacacacgg atatcagggc tatctcccac
    19861 aatataaagg tgcttttacc gttttccggc tgcggtcaat tgtgggacat cgcctataat
    19921 ttgcattatc atacctgtca ttcaatgtcg tgcctaacac cactgacgct attcggacag
    19981 gattaacact agcgtagaga tgacgagtac gttagtctct gaccgtttgt cacgcgcaac
    20041 gttaccgatg atggaacaat aaaatcaacg tatgaaaaaa cgtatcccca ctctcctggc
    20101 caccatgatt gccaccgccc tttatagtca acagggactg gcagccgacc tcgcctcaca
    20161 gtgcatgttg ggcgtgccaa gctatgaccg tcctctggta cagggcgata ccaatgactt
    20221 acccgtgact atcaatgctg accacgcgaa aggggactac ccggatgacg ccgtgtttac
    20281 tggcagcgtg gatatcatgc agggtaacag ccgtctgcag gccgacgaag tgcagctcca
    20341 tcaaaaagag gcaccaggac aaccggagcc ggtacgtacc gttgatgcgc tcggtaatgt
    20401 ccattacgac gataaccagg tgatcctcaa agggccgaaa ggctgggcga atctgaacac
    20461 caaagatacc aacgtctggg aaggtgatta ccagatggtg ggtcgccagg gtcgcggtaa
    20521 agcggacctg atgaaacaac gtggcgaaaa ccgctatacc attctggata acggtagctt
    20581 tacctcctgt ctgccgggtt ctgacacctg gagcgtggta ggtagcgaaa ttattcatga
    20641 ccgcgaagaa caagttgcgg agatctggaa cgcccgcttt aaggtgggtc cggtaccgat
    20701 cttttatagc ccctatttgc agttgccggt gggtgacaaa cgtcgctctg gtttcttgat
    20761 cccgaacgcc aagtacacca ccaccaacta ctttgagttc tacctgccat attactggaa
    20821 catcgcgcca aatatggatg ccaccatcac gccgcattat atgcatcgtc gtggcaacat
    20881 catgtgggag aacgaattcc gctacctctc ccaggcgggc gctggcttga tggaactgga
    20941 ctatctgcct tcagataaag tctatgaaga tgaacacccg aacgatgaca gttcacgtcg
    21001 ttggttgttc tactggcaac actccggggt catggatcaa gtgtggcgtt tcaacgtcga
    21061 ctacaccaag gtcagcgatc ctagctactt caatgatttc gataacaagt acggttccag
    21121 tactgacggc tacgcaacgc aaaaattcag cgttggctat gcggtgcaaa acttcaatgc
    21181 caccgtttca accaagcagt tccaggtctt tagcgagcag aacaccagta gctactcggc
    21241 agagccgcag ttagacgtta actactacca gaatgatgtt ggtccgtttg atacgcgtat
    21301 ttacggccag gcagtgcact ttgttaacac cagagacgac atgcctgaag caacccgtgt
    21361 tcacctggaa ccgaccatca atttgccgct ctctaataac tggggcagca tcaataccga
    21421 agcgaagttg ctggcaaccc attatcagca aaccaatctt gactggtata actccagaaa
    21481 cacgaccaag ctggacgaat ccgttaaccg cgttatgccg caattcaaag ttgacggcaa
    21541 aatggtcttt gaacgcgata tggaaatgct ggctccgggt tatacccaaa cgctggaacc
    21601 gcgcgcgcag tatttgtacg tgccgtatcg cgatcagagc gacatctata actacgactc
    21661 gtctctactg caatctgact actctggcct gttccgggac cggacttacg gcggtcttga
    21721 ccgtattgcc tccgctaacc aggtgacgac cggtgtcaca tctcgcatat atgatgatgc
    21781 tgccgttgaa cgttttaata tttccgttgg tcaaatctac tatttcacgg agtctcgcac
    21841 tggcgatgac aacataacat gggagaatga cgacaaaacg ggctcactgg tgtgggcagg
    21901 cgatacttac tggcgtatct ccgagcgttg gggattgcgt ggcgggattc agtacgatac
    21961 acgtctggat aacgtagcga ccagtaactc cagcattgaa taccgtcggg atgaagaccg
    22021 tctggtacag ctgaattacc gttacgccag cccggaatat attcaggcta cgctgcctaa
    22081 gtactattcc actgctgagc aatataagaa tggtatttcg caggtaggtg ctgtcgccag
    22141 ctggccaatt gccgatcgtt ggtccattgt tggggcctac tactacgaca ccaatgcgaa
    22201 caagcaagcc gactctatgt taggtgtgca atacagctcc tgctgctatg caattcgcgt
    22261 cggttacgag cggaagctga acggttggga taacgataaa caacatgcgg tatatgacaa
    22321 cgcaatcggc tttaacatcg aacttcgcgg cctgagctcc aactacggtc tgggtacgca
    22381 agagatgctg cgttcgaaca ttctgccgta tcaaaacact ttgtgatttg ttgatttacc
    22441 acgtaatccg cagtgcggtt aattgaaatg gaaaaagtat gaagaactgg aaaacgctgc
    22501 ttctcggtat cgccatgatc gcgaatacca gtttcgctgc cccccaggta gtcgataaag
    22561 tcgcagccgt cgtcaataac ggcgtcgtgc tggaaagcga cgttgatgga ttaatgcagt
    22621 cggtaaaact gaacgctgcg caggcaaggc agcagcttcc tgatgacgcg acgctgcgcc
    22681 accaaatcat ggaacgtttg atcatggatc aaatcatcct gcagatgggg cagaaaatgg
    22741 gagtgaaaat ctccgatgag cagctggatc aggcgattgc taacatcgcg aaacagaaca
    22801 acatgacgct ggatcagatg cgcagccgtc tggcttacga tggtctgaac tacaacacct
    22861 atcgtaacca gatccgcaaa gagatgatta tctctgaagt gcgtaacaac gaggtgcgtc
    22921 gtcgcatcac catcctgccg caggaagtcg aatccctggc gcagcaggtg ggtaaccaaa
    22981 acgacgccag cactgagctg aacctgagcc acatcctgat cccgctgccg gaaaacccga
    23041 cttctgatca ggtgaacgaa gcggaaagcc aggcgcgcgc cattgtcgat caggcgcgta
    23101 acggcgctga tttcggtaag ctggcgattg ctcattctgc cgaccagcag gcgctgaacg
    23161 gcggccagat gggctggggc cgtattcagg agttgccggg tatcttcgcc caggcattaa
    23221 gcaccgcgaa gaaaggcgac attgttggcc cgattcgttc cggcgttggc ttccatattc
    23281 tgaaagttaa cgacctgcgc ggcgaaagca aaaatatctc ggtgaccgaa gttcatgctc
    23341 gccatattct gctgaaaccg tcgccgatca tgactgacga acaggcccgt gtgaaactgg
    23401 aacagattgc tgccgatatc aagagtggta aaacgacttt tgctgccgca gcgaaagagt
    23461 tctctcagga tccaggctct gctaaccagg gcggtgatct cggctgggct acaccagata
    23521 ttttcgatcc ggccttccgt gacgccctga cccgcctgaa caaaggtcaa atgagtgcac
    23581 cggttcactc ttcattcggc tggcatttaa tcgaactgct ggatacccgt aatgtcgata
    23641 aaaccgacgc tgcgcagaag gatcgtgcat accgcatgct gatgaaccgt aagttctcgg
    23701 aagaagcagc aagctggatg caggaacaac gcgccagcgc ctacgttaaa atcctgagca
    23761 actaatggtt aaaacccaac gtgttgtgat cactcccggc gagcccgccg ggattggccc
    23821 ggacttagtt gtccagcttg cacagcgtga gtggccggtc gaactggttg tttgtgccga
    23881 tgccactctc cttaccgacc gggcagcgat gctcggtttg ccgctcaccc tccgccctta
    23941 ttcccccaac tcccctgcac aaccgcaaac tgcgggcaca ttaacgctac ttcctgtcgc
    24001 gctacgcgaa tctgtcactg cggggcagtt agcgattgaa aatgggcatt acgtggtgga
    24061 gacgctggcg cgagcgtgtg atggctgtct gaacggtgaa tttgctgcgc tgatcacagg
    24121 ccccgtgcat aaaggcgtca ttaacgacgc aggtattccg tttactggtc ataccgagtt
    24181 tttcgaagag cgctcgcagg cgaaaaaagt ggtgatgatg ctggcgaccg aagaacttcg
    24241 cgtggcgctg gcaacgacgc atttaccgct gcgcgatatc gcagatgcta tcacccctgc
    24301 acttttgcac gaagtgattg ctattttgca tcacgatttg cgtacaaaat ttggtattgc
    24361 cgaaccgcgc attctggtct gcgggctgaa tccgcacgcg ggcgaaggcg gtcatatggg
    24421 tacggaagag atagacacca ttattccggt gctcaacgag ctgcgggcgc aggggatgaa
    24481 actcaacggg ccgctgcctg ccgataccct gtttcagccg aaatatctcg ataacgccga
    24541 cgccgtgctg gcgatgtacc acgatcaggg tcttcccgtg ctaaaatacc agggcttcgg
    24601 gcgcggtgtg aacattacgc tgggcctgcc ctttattcgc acatcagtgg accacggcac
    24661 cgcgcttgaa ctggcgggac gtggcgaagc cgatgtcggc agttttatta cggcgcttaa
    24721 tctcgccatc aaaatgattg ttaacaccca atgaataatc gagtccacca gggccactta
    24781 gcccgtaaac gcttcgggca aaactttctc aacgatcagt tcgtgatcga cagcattgtt
    24841 tctgccatta acccgcaaaa gggccaagca atggtcgaaa tcggccccgg tctggcggca
    24901 ttgaccgagc cggtcggcga acgtctggac cagctgacgg tcatcgaact tgaccgcgat
    24961 ctggcggcac gtctgcaaac gcatccattc ttaggcccga aactgacgat ttatcagcag
    25021 gatgcgatga cctttaactt tggcgaactg gccgagaaaa tgggtcagcc gctgcgtgtt
    25081 ttcggcaacc tgccttataa catctccacg ccgttgatgt tccatctgtt tagctatact
    25141 gatgccattg ccgacatgca ctttatgttg caaaaagagg tggtgaatcg tctggttgca
    25201 ggaccgaaca gcaaagcgta tggtcgatta agcgtcatgg cgcaatacta ttgcaacgtg
    25261 atcccagtgc tggaagtacc gccgtcagcc tttacaccac cacccaaagt ggattccgcc
    25321 gtcgtgcgcc tggttcctca tgcaacgatg cctcacccgg ttaaagatgt tcgcgtgttg
    25381 agccgcatca ccactgaagc ctttaaccag cgtcgtaaaa ccattcgtaa cagcctcggc
    25441 aacctgttta gcgtcgaggt gttaacggga atggggatcg acccggcgat gcgagcggaa
    25501 aatatctctg tcgcgcaata ttgccagatg gcgaactatc tggcggagaa cgcgcctttg
    25561 caggagagtt aacgatgatc aattcgcccc gagtgtgtat tcaggttcaa agcgtctaca
    25621 ttgaggcaca atcttcacct gataatgaac gttacgtttt tgcttatacc gtaaccatac
    25681 gcaatctggg gcgagcgcca gtgcagttgt tggggcgtta ctggctgatc accaatggca
    25741 atggccgtga aaccgaagtc cagggcgaag gagtggttgg cgtccagcca cttatcgcgc
    25801 ctggcgaaga gtaccagtac accagcggtg caatcattga aaccccgctg ggcaccatgc
    25861 agggtcacta cgaaatgatc gatgaaaatg gcgtcccttt cagcatcgac attcccgtat
    25921 tccgactcgc cgttcccaca ctcattcatt aaaagaatat ggcgacatac cttattggcg
    25981 acgttcatgg ttgttacgat gaactgatcg cattgctgca taaagtagaa tttacccctg
    26041 ggaaagatac cctctggctg acgggcgatc tggtcgcgcg cgggccgggt tcgctggatg
    26101 ttctgcgcta tgtgaaatcc ttaggcgaca gcgtacgtct ggtgctgggc aatcacgatc
    26161 tgcatctgct ggcggtattt gccgggatca gccgcaataa accgaaagat cgcctgacac
    26221 cgctgctgga agcgccggat gccgacgagc tgcttaactg gctgcgtcgc cagcctttgc
    26281 tgcaaatcga cgaagagaaa aagttggtga tggcccacgc agggatcacg ccgcagtggg
    26341 atctacagac cgccaaagag tgcgcgcgcg atgtagaagc ggtgctatcg agtgactcct
    26401 atcccttctt tctcgacgct atgtacggcg atatgccaaa taactggtca ccggaattgc
    26461 gggggctggg aagactgcgt tttatcacca acgcttttac ccgtatgcgt ttttgcttcc
    26521 cgaacggtca actggatatg tacagcaaag aatcgccgga agaggcccct gccccactga
    26581 aaccgtggtt tgcgattcct ggccctgtcg ctgaagaata cagcatcgcc tttggtcact
    26641 gggcatcgct ggagggcaaa ggtacgccgg aaggtatata cgcgctggat accggctgct
    26701 gctggggtgg tacattaacc tgcctgcgct gggaagataa acagtatttt gtccagccgt
    26761 cgaaccggca taaggatttg ggcgaagcgg cggcgtctta aacacagcct gatataggaa
    26821 ggccggataa gacgcgaccg gcgtcgcatc cggcactagc cgtaaattct atacaaaatt
    26881 accgccgctc cagaatctca aagcaatagc tgtgagagtt ctgcgcatca gcatcgtgga
    26941 attcgctgaa taccgattcc cagtcatccg gctcgtaatc cgggaaatgg gtgtcgcctt
    27001 ccacttctgc gtcgatatgc gtcagataca gtttctgcgc ttttggcagg aactgctcat
    27061 aaacgcgacc gccgccaatc accatgattt ctggtacgtc accacacgcc gcgatggctt
    27121 catccaccga cttcacccac gttacgcgat cgtccgtacc cggttgactg ctgaggataa
    27181 tatttttgcg tcctggcaac ggacgaccga ttgattccca agtatggcgg cccataatca
    27241 cgggtttatt taaggtgttg cgtttaaacc aggcgagatc ggcaggcagg ttccacggca
    27301 tggcgttttc catgccgata acgcgatcta ccgctaacgc cgcaatcaga ctgatcattg
    27361 agatttcccg ataaaaaaaa ttgtcgccac tatacgtaaa gcgtaaaccg tcgtcgactg
    27421 gtgcgaggat gatgttgagg aaaattttat attctgctgg cgagtccacg ctctctccct
    27481 ggactcgccg cattacaatg aaacaaaaac aaacagttag ctgtaaagtg tgatttacgt
    27541 cactctttat taggatgagg gtttcgtttc cggttcatcc gccatgttgc cggtatgttt
    27601 accttcttcg gttccctgcc agccatgtcg ttgaattaat gacagatgtt cgcggtcctc
    27661 ggtaatgatc tcacttaaca tcgcgctggt gcgtttatag accgccgcgc gggctttggt
    27721 gtcgttctca accattgcca tctcttccac catctgaata ttaaagcggc ggaacacatc
    27781 ggcacgttct cgcgcttcat acggccccag acctaaactt tccagtgcca gacgcccggt
    27841 tttcagcgca ccttcgaagg tttcacgctc cggcttttca acgcctgcct gacgcaaacg
    27901 aatgtagtgg tcgacatcgc gggcgcgggc aataatctgc aaatgcggga agtgttcttt
    27961 caccatctct gtcagttgca ggttggtttg cggatcgtcg atggcgttaa tcagcacttc
    28021 cgctttcgcc gctcccgcag attccagtaa atccatccgc gtggcatcgc cataaaacac
    28081 tttcatacca aatttacgca aggtttcgat atggtccgga tcgtgatcga gcaccaccat
    28141 tttcaccccg ctggagagca gtaaacgtcc ggtgatctgc ccaaaacgac cgaatccggc
    28201 aatgattacc cgcggctgtt cttcgtcgat ctcatcagct tcacgcgcct cttcagtaga
    28261 agattgctca aggcgattga ggatcaccag cagaatcggc gttgccgcca tcgacagcgc
    28321 caccgccagg gtcagcgatt tcgcccactc cggttccagc acattcgcca tctgcgccgt
    28381 gccaaatacc acaaaggcaa actcactgcc ctgccctaac aacaccgcaa accaacgacg
    28441 ctgtttattt ggcacttgca acggtcgggc aatcagccac agcatggcga ttttgatgat
    28501 gaggaaaccg agcagcaaaa tgacaatgcg caatgggttt tcaagcagcg tgccaaagtc
    28561 tatcgacatg ccaacaccga tgaaaaacag ccccaacagc aaacctttaa atggttcgat
    28621 atcgctctcc agcgcatgac ggtattccga gcttgccagc agtacgcccg ccagaaacgc
    28681 gcccatcgcc atcgacaagc cgacctcttc cagcagcaaa ccaaagccaa acacgaggaa
    28741 taacgccacg gcactaaaca cttcccgcaa gccagagcgg gctacaaaac gcagcgccgg
    28801 acgcgtgaca tagcgcccca gcaataccac cagcaccagc gcacccgcca cttttaacgc
    28861 cgagagagca aatgcgccca tcgtcgtcga ggcactgctc gttgccagta gcggaatcat
    28921 cgccaccagc gggatcgccg cgatatcctg gaacagcagc accgcaaagg cactgcgacc
    28981 catttgcgtc accatcagat tgcgttcatt catcgcctgc atggcaatcg ccgtagagga
    29041 gagcgccagc gtcatgccga tcaattccgc gacctgccag tgcagcccaa gtaacatgca
    29101 gaacagcccc agcaggccgc cgcaaatcac catctgcaat gcgccaccgc cgaacactgc
    29161 cgcacgcagc ttccacagcc tttgtggatc gagttcgagg ccgataataa acagcatcag
    29221 caccacccca atctcggcaa agtgcagaat agattcggca tcggtcacca gtcgcagccc
    29281 ccacgggcca ataatgcagc cggcgatcag gtagccaagt accgatccca gaccaagacg
    29341 taccgcaatg ggtacaatca gcgctgccga accgagataa atcagcgcct gaatcagcgt
    29401 atggctatcc atgatgggcc tcctgccatt ccagcagacg ttgcttatag tgacgcgcct
    29461 gcccttcgag ggtttcgtcg tcacaaataa aggtgcagtg catggcaaac ggtggcagcc
    29521 agttcagccc gcagtagatt gccgtcgcct gtagcggctg cgacagcaca tcaaagcccg
    29581 gatgcgcacc aatttcaaaa tggctttccc cgccgccggt cgtcaccgcc cacagcaaat
    29641 atttgccatg cagcgccttg ccgccgtgac cgtaagccca gccgtgcgac aaaactttat
    29701 cgatccaaag tttaagaagc ggaggaatgc tgtaccactg catcggatgc tgccagacga
    29761 tcagatcggc gcgagacagc gcctcctgct cggcggcaat atcgatattg aagtcaggat
    29821 agagttgata aagagagcga atttcgacgc cttccagcgt ccttgcctgt tcaagcatcc
    29881 gtttattcgc atgggaatga tgcggatacg gatgcgcata aattataaga atcataggta
    29941 gcccgcgtga ttacgccttt gttttcccac aaagtgtagt catatattca cgcggcttat
    30001 agtcaataat attgattcag aaaagagaga aatctgaaca atttacccca ttctctgccc
    30061 gcggatcgtc atattgctgg tttgcgccag tgacatccct cgagtctccg gggcaaacgc
    30121 tacggaaatc aacaagccaa acagcgagat acccgcctcc attagcatcg tgttactgat
    30181 accgtaatta ttgataaaga tcggcagtgc ccaggtcgaa acaatggtgc caatacggct
    30241 taaggacata atcacgccca cggcagaggc gcggatatct gtcgggaaga gttcattagg
    30301 atagagccac tgcaaattac ccggcccgcc agagaaaaag gcatacaccg caaaggccat
    30361 cactaccagc cagatcccca tatccgggat cagccccaaa accgccagcg ccagcgtcat
    30421 catggcaaag ctgccaatca acaatggacg ccgtccggca gtgtttaacc acagcatcgg
    30481 cggaatacag ccgagcataa agaacaggct aatcaccacg ttccccagcg ccgcgttttt
    30541 gccaacaccc aatcccaaca aaccaacgat ttgcgggcca aaggtgtaaa tggcgaacat
    30601 tgggatcacc tggcaggtcc agatggcggc aacaaacaga acaaaaggaa aatggcggcg
    30661 attaaacaga tcgcgaaaac gggtttgcta cggctgctct tcatcgaaag ccaccggttc
    30721 gccaaacagt tttatcatca tctcttcgca ctcttttact cgccctttgc gtaataacca
    30781 gcggggagat tcaggcagtt cgaatcgacc aatcaaaatc aacaaacagg ggatcgccgc
    30841 gctacccagc atccagcgcc agccgccttc cacatcataa agccagtagc cgaccagatc
    30901 ggcgcaggtc gcgccgacat accacatggc ggcgataaag ctgatggaaa atgcccgctg
    30961 acgggtactg gagaactcgg tgatcattga ggtggcgatg ggataatctg caccgatgac
    31021 aatgccgata agtacccgca tcaccaacag ttcgacgggg gatgaaacaa acatcgtcgc
    31081 caccgatatc acgccgatgg cgatgatatc aatgaggaac attttgcgcc gtcctacttt
    31141 atcggaaata tagccaaaca gcgatgtgcc aacgaacagc ccggcgagcg ttcccgcgcc
    31201 caacaagcca atccagtcag cgtccagttt tagcgccgga gtcagttgct ccagcgccac
    31261 gccaatcatt accagtacat aaccatccag aaacggacca ccgcttcccc acagcaaaat
    31321 gcggcggtga attgaggaga atttgagatc gtcaaagttt ctggacggtt gcatgggtta
    31381 ttcctgtttt tttgcaatga gagtcagtac ggcgttacgt atcaaaccaa catcagccgt
    31441 aacggaactc cacaccaaag gtgccgcgcg ggtattccca ctgttccagc gccgacccca
    31501 gccccagaat gcgacaggtg ccgcactcca gacatccggc gtaatcgaag cgcacactgc
    31561 cgtcatcctg cttcttgtac agacctgcgg ggcacgcttt caccagcagc tccagggcct
    31621 gtttatcggc atcggccttt acaacaatgt gcggatgctc ttcatcgaca ttgaatttat
    31681 tgacgcccag tttgacgtcc acattgacgg gagaagtcat aaaacggtca ctcctttcat
    31741 gccatccttg atcagattga tgaagcccac tttcttgccg tggcggagga ttttcttgcg
    31801 catcagttcc ggcgcactgc cgtcaatggt gaacaggtca cgcgccacgc ccaccgccag
    31861 ttccgggtag ccgctaaaca tgcgtgggtt atcaaggaag gccggtagtt tctggtacat
    31921 acgcatatcg cgcagcgggc cactctcaag atgctgacga tattccgcca gtttttgctt
    31981 actgaaatcg tcgcttttca tcgctgaaag cacggttttt gctgcggctt ccccggcggc
    32041 aatcgccaga tccataccgc gaatggtaaa accgaggttc atacacattc cggcggcatc
    32101 accggcaatc aatacgccgt caccaaccaa ctccggcagc atgttgatgc ctgcttccgg
    32161 cactacgtga gcggaatatt ccaccagctt gccgcccgcg atcagcggtg ccactgccgg
    32221 atgctgtttg aaatcttcca gcatttgcgg caccgatttt tttgcgtcat gcagatgatg
    32281 cagaccacaa accagcccca gcgacagggt attttcattg gtataaagga agccgccgcc
    32341 catcaggcca tcggtgggtg atcccgcaaa cagacaagcc gcgccctgat taccctgcaa
    32401 ctgaaaacgg tcttcgatta ccgacttcgg taactcgatc agttccttca cgccaaccgc
    32461 cacatccgtc ggtttaacgc gttttgccat ccccagcttt tcggcaagga tggagttcac
    32521 tccatcagca aggatcaccg ttttcgcttc aatcacatcg ccatcggctt ctacaccgac
    32581 gactttgcca tcgcgctgta cgaggttatc gacgcggatc ccggtaatta actgcgcgcc
    32641 cgcttcttcg gcctgctcca tcagccaggc atcaaattta ctgcgcaaaa cggagtaaga
    32701 acgctgggat ggcgaggttt cgtcaccgtt gcagtagtcc atagtcatcg ctgacttttc
    32761 cgtcataaac gcgagttttt catgggtgat caggcgttct accggggcgg attcggcgaa
    32821 accaggaata atgtgttcca ggctgtgggc atagagacgc ccgccggtga cgttcttggc
    32881 acctgcggaa ttgccacgct cgataactaa cacttgcgcc ccttcgcggg cgagcaccag
    32941 tgcggcaacc gaaccggcaa gcccagcacc gacgatgatg gcgtcaaaga tatcttcgga
    33001 cataagagct ccctgtcagc ggcagggcca aaatgcagcc ctgccagagt ggatcaacgc
    33061 gctaaagctg cggtcagcgc cggaaggatc ttcacggcgt caccaacaat gccgtaatcc
    33121 gcgtactgga agatcggcgc atttttatct ttattgatgg cgaaaatggt ttgcgatgca
    33181 ttagcaccaa ccatgtgctg gatctgcccg gagatcccca ccgccaggta cagttcaggt
    33241 ttcagcatca ggttggagat accgacatag cgttcgtgct ccatccattt ttcgttttcc
    33301 gccaccggac gagaacaggc caactccgca cctatcgcct tgcaaagctg ttctgccagc
    33361 gcaatgttct ctttgctgcc aataccgcga ccgacgctga ccaccagacg ggctttgtcg
    33421 agatcgacgc tgttgctctg gcgcgcctgg gttgccgtgc gggtaatcgc cacagccgga
    33481 gcctgccact ccacggtgtg cgtttcgcca gtgcgtgacg cgtctggctg agccgcatcg
    33541 aacgtgccgc tgctgatggt cagtaccgca tacggcgtgg caatgcgttc ttcgccaatc
    33601 gccagaccac cgtaaaccat atgtttcact gttgctttac cgtcctgtac gctgacggtg
    33661 ctggcatcgt tagacaccgc cgctttaagg cgataaccca gttttgccgc cagtaatttg
    33721 ccgcgacggg tgtttggcag cagcaccagg ccgtctgcgc cgtgctggcg aatagtgtca
    33781 gccatgacac cggcgtaatc ttcgatcatc cgatcgtccg gtttgccgtt taatttccag
    33841 acatgattag cgccgagctg gattgcctgt gcgccgtcgg catcattgag gacaaaggtg
    33901 ttgatttgat tagctaaagc ctgcgcaccg ttcatcagtt ccggcagacg agaaggggta
    33961 tcgctgaata cccagacttg agaaaacgtg ttcatagcat cccctgtaat taaatgactt
    34021 tgcgaagatt ttcagcaaat gcggcgatct gttcttcgcc gtcgccttca atcacgatgc
    34081 gctgacgttc gcgctgtttc ggcgcggcaa cctgttgttc tgaccaggct gcctctgcgt
    34141 taaaaccaat atccgccgcc gaccatacct ggacgggctt tttcgccgcg ccgagaatgg
    34201 ctttcatcga aggaatttgt ggggaattga tatcagtgga aacagcaaca accgcaggca
    34261 gcggaatgct taaggtttcg gtttcatctt ccagttcgcg ctcaacggtg agggtatctg
    34321 ccgtcaggga gataattttg ctgacgccgt taactgccgg aatattgagg atttcgccca
    34381 ccagcagacc aacctgctgg gcataaaggt cggaagaacc atcgccacag aggatcaaat
    34441 caaagcctgc tttctgggcg gctgcagcca gtgcgctcgc cgtttgttgc ggcagtgcct
    34501 gctcgaactg gtcatcaatc accacaatca gttcatccgg gccgcgcgat agtacatctt
    34561 tacgcccttt ggcgttggtc agggctttac cgcccacact taaggccgtc acctgcgcct
    34621 ctgctgcctg ttgctttagc tggcaagccg cttcaatagc gttcagatcg tattggctta
    34681 ttttggcatc ggctttgctg aagtctaatg aaccatcagc attattgacc gcaatatcct
    34741 gttcatcagg cacgcactta tagcaagtaa taatcttcat tgcatctcca gaaatcatga
    34801 aggaaacatc gttcatatcg gtggtgttaa ttacagatat cacgcccttt aatttggaaa
    34861 taaaacttta atcaccaata ttgaaaatgt cacacgcaga taaatccaac tttcaatatt
    34921 gttaagttcc tcaccaatat tgaaaacatg gcgtagaaaa aagaaatttt caatattgct
    34981 ttatggatca ccaatattga aagtcagatg ctattttttt actcacaaca caacataaca
    35041 aactgattaa taatcaaaat taacgagaaa agcgccctct attcacccga aaaaaaatgt
    35101 gataacaatc acagaatgca gcttattgaa tacccattat gagttagcca ttaacgcgtc
    35161 cacgaggtta ataataatta tatcaaatgt taacaaaaat aaaacaaacg ggaacgcaaa
    35221 ataaatattc gttttcacag tggaattaac tcatgaagaa tgaaaagaga aaaacgggaa
    35281 tagaaccgaa ggttttcttt ccgccgttaa taatcgtcgg catactttgt tggcttacag
    35341 tcagagatct ggatgcagcg aatgtcgtta ttaatgctgt attcagttac gtcaccaatg
    35401 tatggggatg ggcatttgaa tggtatatgg tggtgatgct tttcggttgg ttctggctgg
    35461 tgtttggccc gtatgccaaa aagcgtttag gtaacgaacc acctgaattt agtaccgcca
    35521 gttggatctt tatgatgttc gcctcctgta cgtctgctgc cgtactgttc tggggatcga
    35581 ttgagatcta ctactacatc tccaccccgc cgtttggctt agaaccgaac tcgacagggg
    35641 cgaaagagtt ggggctggct tacagcttgt tccactgggg acctctgccg tgggccactt
    35701 acagcttcct ttcagtcgcc ttcgcttact tcttctttgt ccgcaaaatg gaagtgattc
    35761 gccccagctc gacactggtg ccgctggtag gtgaaaaaca cgccaaaggg ttgttcggca
    35821 ctatcgtcga caacttctat ctcgtcgcct tgatcttcgc gatgggtacc agtctgggcc
    35881 ttgccacgcc gctggtgacc gagtgtatgc aatggttgtt tggcattccg cataccctgc
    35941 aactggacgc tatcatcatt acctgctgga ttatcctcaa cgccatttgc gtcgcttgcg
    36001 gtctgcaaaa aggggtacgt atcgccagtg acgtgcgtag ttacctgagc ttcctgatgc
    36061 tgggttgggt gttcattgtc agcggtgcca gcttcatcat gaactacttc accgattcgg
    36121 tggggatgtt gctgatgtat ctgccgcgca tgttgttcta taccgatccc atcgctaaag
    36181 gcggcttccc gcagggctgg accgtgttct actgggcatg gtgggtgatt tatgctatcc
    36241 agatgagtat cttcctcgcc cgcatctccc gtggtcgtac tgtgcgtgaa ctgtgcttcg
    36301 gcatggtgct ggggctgaca gcgtcaacct ggatcctgtg gactgtactc ggtagtaaca
    36361 ctctgctgtt gatagataaa aacatcatca acattccaaa tctgatcgaa cagtacggtg
    36421 tggcgcgcgc catcattgaa acctgggccg ctctgccact cagcaccgcc accatgtggg
    36481 gcttcttcat cctctgcttt attgccaccg ttacactggt taacgcctgc tcttataccc
    36541 tggcgatgtc cacttgccgc gaagtacgcg atggtgaaga accacctctg ctggtgcgta
    36601 tcggttggtc aattctggtt ggcattatcg gtattgttct gctggcgctc ggcggcctga
    36661 aaccgattca aaccgccatt atcgccggag gatgcccgct gttcttcgtc aacattatgg
    36721 tgacgctctc ctttattaaa gacgcgaaac agaactggaa agattaatta acccccaaaa
    36781 tatcaagagg ttgaaagatg gattttaatt taaatgatga gcaggaactg tttgtcgccg
    36841 gtatccgcga actgatggcc agcgaaaact gggaggccta ttttgccgag tgcgaccgtg
    36901 acagcgtcta cccggaacgt tttgtcaaag cactggcgga tatgggtatc gacagtctgc
    36961 tgatccctga agagcacggt ggtctggacg cggggtttgt tactctcgcc gccgtgtgga
    37021 tggagctggg acgtctgggg gcaccaacct atgtgctgta ccagttgccg ggcgggttca
    37081 acaccttcct gcgcgaaggc acacaagagc agatcgacaa aattatggct ttccgcggca
    37141 ccggtaagca gatgtggaac tcagcgatta ccgaaccggg cgcgggctcc gacgtgggta
    37201 gcctgaaaac gacttatacc cgtagaaatg gtaagattta tcttaatggt agtaagtgtt
    37261 ttattaccag cagcgcctac accccgtaca tcgtggtgat ggcgcgcgac ggggcttctc
    37321 cggacaaacc tgtctacacc gaatggtttg ttgatatgag caaaccgggc atcaaagtga
    37381 ccaaacttga aaagctcggt ctgcgtatgg atagctgctg tgaaatcacc tttgacgacg
    37441 tggaactgga cgagaaagac atgttcggtc gggaaggtaa cggctttaac cgcgtcaaag
    37501 aagagttcga ccatgaacgt ttcctggtag ccctcaccaa ctacggtacg gcgatgtgcg
    37561 cttttgaaga tgcggcgcgc tacgccaatc agcgcgtgca gtttggcgag gctattggtc
    37621 gtttccagtt gattcaggaa aaattcgccc acatggcgat caaattaaac tccatgaaaa
    37681 acatgctgta tgaagcagcg tggaaagcag acaacggcac catcacctct ggcgatgcag
    37741 cgatgtgcaa atacttctgc gccaatgcgg catttgaagt tgtggatagc gcaatgcagg
    37801 tgctgggcgg tgtcgggatt gcgggcaacc accgcatcag ccgcttctgg cgtgatctgc
    37861 gtgtagaccg cgtctccggg ggatctgacg aaatgcagat cctgacgctg ggtcgcgcgg
    37921 tgctgaagca ataccgctaa gtccgtgcgc tgcctgatgc gacgctaatg cgtcttatca
    37981 ggcctacggg agcacgaatg taggccggat aaagcgttta cgccgcatcc ggcagtcatg
    38041 cactacataa atttttcagg agctaataat ggatcatcta cccatgccga aattcgggcc
    38101 gttggccgga ttgcgcgttg tcttctccgg tatcgaaatc gccgggccgt tcgccgggca
    38161 aatgttcgca gaatggggtg cggaagttat ctggatcgag aacgtcgcct aggccgacac
    38221 cattcgcgtt caaccgaact acccgcagct ctcccgccgc aatttgcacg cgctgtcgtt
    38281 aaatattttc aaagatgaag gccgcgaagc gtttctgaaa ttaatggaaa ccaccgatat
    38341 cttcatcgaa gccagcaaag gtccggcctt tgcccgtcgt ggcattaccg atgaagtact
    38401 gtggcagcac aacccgaaac tggttatcgc tcacctgtcc ggttttggtc agtacggcac
    38461 cgaggagtac accaatcttc cggcctataa caccatcgcc caggccttca gtggttacct
    38521 gattcagaac ggtgatgttg accagccaat gcctgccttc ccgtataccg ccgattactt
    38581 ttctggcctg accgccacca cggcggcgct ggcagcactg cataaagtgc gtgaaaccgg
    38641 taaaggcgaa agtatcgaca tcgccatgta tgaagtgatg ctgcgtatgg gccagtactt
    38701 catgatggat tacttcaacg gcggcgaaat gtgcccgcgc atgagcaaag gtaaagatcc
    38761 ctactacgcc ggttgcggtc tgtataaatg cgccgacggc tacatcgtga tggaactggt
    38821 gggcattacc caaattgaag agtgctttaa agatattgac ctcgcacatc tactcggtac
    38881 gccggaaatc ccggaaggca ctcagcttat ccaccgtatc gaatgccctt acggcccact
    38941 ggtagaagaa aaactcgatg cctggctggc ggcacatacc atcgcagaag ttaaagaacg
    39001 ctttgccgaa ctgaatatcg cctgtgccaa agtactgacc gtaccggaac tggaaagcaa
    39061 tccacagtat gttgcccgtg aatcaatcac tcacatggca tggacacggc tgccattttg
    39121 aaaaatatcg gctacagcga aaacgacatt caggagttgg tcagc
//
LOCUS       DADMKF010000021        38337 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.021, whole genome shotgun sequence.
ACCESSION   DADMKF010000021 DADMKF010000000
VERSION     DADMKF010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 38337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38337
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.021"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            59..1423
                     /locus_tag="JNC24_000988"
     CDS             59..1423
                     /locus_tag="JNC24_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY9157803.1"
                     /translation="MNDYKMTPGERRATWGLGTVFSLRMLGMFMVLPVLTTYGMALQG
                     ASEALIGIAIGIYGLTQAVFQIPFGLLSDRIGRKPLIVGGLAVFAAGSVIAALSDSIW
                     GIILGRALQGSGAIAAAVMALLSDLTREQNRTKAMAFIGVSFGITFAIAMVLGPIITH
                     KLGLHALFWMIAILATTGIALTIWVVPNSSTHVLNRESGMVKGSFSKVLAEPRLLKLN
                     FGIMCLHMLLMSTFVALPGQLADAGFPAAEHWKVYLATMLIAFGSIVPFIIYAEVKRK
                     MKQVFVFCVGLIVVAEIVLWNAQTQFWQLVVGVQLFFVAFNLMEALLPSLISKESPAG
                     YKGTAMGVYSTSQFLGVAIGGSLGGWIDGMFDGQGVFLAGAMLAAVWLAVASTMKEPP
                     YVSSLRIEIPADIAANEALKVRLLETEGVKEVLIAEEEHSAYVKIDSKVTNRFEVEQA
                     IRQA"
     gene            complement(1551..2042)
                     /gene="yajQ"
                     /locus_tag="JNC24_000989"
     CDS             complement(1551..2042)
                     /gene="yajQ"
                     /locus_tag="JNC24_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HAY9157804.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            2210..3121
                     /gene="panE"
                     /locus_tag="JNC24_000990"
     CDS             2210..3121
                     /gene="panE"
                     /locus_tag="JNC24_000990"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAY9157805.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            3084..3674
                     /gene="yajL"
                     /locus_tag="JNC24_000991"
     CDS             3084..3674
                     /gene="yajL"
                     /locus_tag="JNC24_000991"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HAY9157806.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLLVRGGIKVTTASVASDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGAECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKTHEVASQLVMAAGIYNYYE"
     gene            complement(3728..5176)
                     /gene="thiI"
                     /locus_tag="JNC24_000992"
     CDS             complement(3728..5176)
                     /gene="thiI"
                     /locus_tag="JNC24_000992"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HAY9157807.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDEKQRLAIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPDVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSVDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNR
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            5382..5624
                     /gene="xseB"
                     /locus_tag="JNC24_000993"
     CDS             5382..5624
                     /gene="xseB"
                     /locus_tag="JNC24_000993"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HAY9157808.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDTSLPPFTPDNE"
     gene            5624..6523
                     /gene="ispA"
                     /locus_tag="JNC24_000994"
     CDS             5624..6523
                     /gene="ispA"
                     /locus_tag="JNC24_000994"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HAY9157809.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDADMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQSLKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            6548..8410
                     /gene="dxs"
                     /locus_tag="JNC24_000995"
     CDS             6548..8410
                     /gene="dxs"
                     /locus_tag="JNC24_000995"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HAY9157810.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVTAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLAI
                     LNFGTLMPDAAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            8465..9439
                     /gene="yajO"
                     /locus_tag="JNC24_000996"
     CDS             8465..9439
                     /gene="yajO"
                     /locus_tag="JNC24_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HAY9157811.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSINDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALKLQKQHGWAQFISMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHAVVGFK"
     gene            complement(9493..10011)
                     /gene="pgpA"
                     /locus_tag="JNC24_000997"
     CDS             complement(9493..10011)
                     /gene="pgpA"
                     /locus_tag="JNC24_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HAY9157812.1"
                     /translation="MTILPRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(9989..10966)
                     /gene="thiL"
                     /locus_tag="JNC24_000998"
     CDS             complement(9989..10966)
                     /gene="thiL"
                     /locus_tag="JNC24_000998"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HAY9157813.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTFDWKGYDHFATP"
     gene            complement(11044..11463)
                     /gene="nusB"
                     /locus_tag="JNC24_000999"
     CDS             complement(11044..11463)
                     /gene="nusB"
                     /locus_tag="JNC24_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HAY9157814.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(11483..11953)
                     /gene="ribE"
                     /locus_tag="JNC24_001000"
     CDS             complement(11483..11953)
                     /gene="ribE"
                     /locus_tag="JNC24_001000"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HAY9157815.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(12042..13145)
                     /gene="ribD"
                     /locus_tag="JNC24_001001"
     CDS             complement(12042..13145)
                     /gene="ribD"
                     /locus_tag="JNC24_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HAY9157816.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGGVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVAAMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRQRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSSEWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGALLQAGLVDELIVYIAPKLLGSDARGLC
                     TLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(13149..13598)
                     /gene="nrdR"
                     /locus_tag="JNC24_001002"
     CDS             complement(13149..13598)
                     /gene="nrdR"
                     /locus_tag="JNC24_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HAY9157817.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDDVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            13749..14288
                     /locus_tag="JNC24_001003"
     CDS             13749..14288
                     /locus_tag="JNC24_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HAY9157818.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     QETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVDQLGFVRIHDIQPVMQ"
     gene            14587..15471
                     /locus_tag="JNC24_001004"
     CDS             14587..15471
                     /locus_tag="JNC24_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HAY9157819.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYDAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(15648..15995)
                     /locus_tag="JNC24_001005"
     CDS             complement(15648..15995)
                     /locus_tag="JNC24_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease family protein"
                     /protein_id="HAY9157820.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(16124..17095)
                     /gene="secF"
                     /locus_tag="JNC24_001006"
     CDS             complement(16124..17095)
                     /gene="secF"
                     /locus_tag="JNC24_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HAY9157821.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(17106..18953)
                     /gene="secD"
                     /locus_tag="JNC24_001007"
     CDS             complement(17106..18953)
                     /gene="secD"
                     /locus_tag="JNC24_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HAY9157822.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(18981..19313)
                     /gene="yajC"
                     /locus_tag="JNC24_001008"
     CDS             complement(18981..19313)
                     /gene="yajC"
                     /locus_tag="JNC24_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HAY9157823.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(19336..20463)
                     /gene="tgt"
                     /locus_tag="JNC24_001009"
     CDS             complement(19336..20463)
                     /gene="tgt"
                     /locus_tag="JNC24_001009"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HAY9157824.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSVYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(20519..21589)
                     /gene="queA"
                     /locus_tag="JNC24_001010"
     CDS             complement(20519..21589)
                     /gene="queA"
                     /locus_tag="JNC24_001010"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HAY9157825.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            21682..22263
                     /gene="acpH"
                     /locus_tag="JNC24_001011"
     CDS             21682..22263
                     /gene="acpH"
                     /locus_tag="JNC24_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HAY9157826.1"
                     /translation="MNFLAHLHLAHLAESSLSGNLLADFVRGNPEESFPPDVVAGIHM
                     HRRIDVLTDNLPEVREAREWFRSETRRVAPITLDVMWDHFLSRHWSQLSPDFPLQEFV
                     CYAREQVMTILPDSPPRFINLNNYLWSEQWLVRYRDMDFIQNVLNGMASRRPRLDALR
                     DSWYDLDAHYDALETRFWQFYPRMMAQASHKAL"
     gene            complement(22268..24085)
                     /gene="malZ"
                     /locus_tag="JNC24_001012"
     CDS             complement(22268..24085)
                     /gene="malZ"
                     /locus_tag="JNC24_001012"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HAY9157827.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVSMHKHRSQQQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLGLDGVFNHSGDSHAWFDRHNRGTGGACHNPES
                     PWRDWYSFSDDGTALDWLGYASLPKLDYQSESLVNEIYRGEDSIVRHWLKAPWSMDGW
                     RLDVVHMLGEAGGARNNMQHVAGSTEAAKETQPEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDIARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     TALFALYQRMIALRKKSQALRHGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEACEV
                     VLPASPLLNAVQWQCKERHGQLTDGILALPAISATVWMN"
     gene            complement(24241..25614)
                     /gene="proY"
                     /locus_tag="JNC24_001013"
     CDS             complement(24241..25614)
                     /gene="proY"
                     /locus_tag="JNC24_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HAY9157828.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVWFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRCLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            complement(25690..27009)
                     /gene="brnQ"
                     /locus_tag="JNC24_001014"
     CDS             complement(25690..27009)
                     /gene="brnQ"
                     /locus_tag="JNC24_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HAY9157829.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVAAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFRLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(27416..28711)
                     /gene="phoR"
                     /locus_tag="JNC24_001015"
     CDS             complement(27416..28711)
                     /gene="phoR"
                     /locus_tag="JNC24_001015"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HAY9157830.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEESGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTQLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            complement(28769..29458)
                     /gene="phoB"
                     /locus_tag="JNC24_001016"
     CDS             complement(28769..29458)
                     /gene="phoB"
                     /locus_tag="JNC24_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HAY9157831.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDCAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            29648..30850
                     /gene="sbcD"
                     /locus_tag="JNC24_001017"
     CDS             29648..30850
                     /gene="sbcD"
                     /locus_tag="JNC24_001017"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HAY9157832.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETTQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIITSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFTTTLH
                     TLAGEHEA"
     gene            30847..33990
                     /gene="sbcC"
                     /locus_tag="JNC24_001018"
     CDS             30847..33990
                     /gene="sbcC"
                     /locus_tag="JNC24_001018"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HAY9157833.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVTLLTPEQV
                     QSLTASLQVLTDEEKQLITAQQQEQQSLNWLTRQDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHIRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVASALAQHSEQRPLRQRLVALHGQIVPQQKRLAQLMVTIQ
                     NVTLEQTQRNVALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTTSLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALTGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLLQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            34116..35300
                     /gene="araJ"
                     /locus_tag="JNC24_001019"
     CDS             34116..35300
                     /gene="araJ"
                     /locus_tag="JNC24_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HAY9157834.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMGVLTELAHNVGISIPAAGHMI
                     SYYALGVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQEFSWRYTF
                     LLIAVFNIAVMASVYFWVPDIRDEAKGNLREQFHFLRSPAPWLIFAATMFGNAGVFAW
                     FSYVKPYMMFISVFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIV
                     LALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFN
                     LGSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKP
                     LG"
     gene            complement(35443..36351)
                     /gene="mak"
                     /locus_tag="JNC24_001020"
     CDS             complement(35443..36351)
                     /gene="mak"
                     /locus_tag="JNC24_001020"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HAY9157835.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVALNGRAHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVGQLIKQFVFGGE
                     CETPVRKAKHGDSSGVRGAAWLWPQE"
     gene            36476..37387
                     /gene="rdgC"
                     /locus_tag="JNC24_001021"
     CDS             36476..37387
                     /gene="rdgC"
                     /locus_tag="JNC24_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HAY9157836.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWIDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFALRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(37465..37920)
                     /locus_tag="JNC24_001022"
     CDS             complement(37465..37920)
                     /locus_tag="JNC24_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9157837.1"
                     /translation="MTQHPWSKLQREIYDLLTPTINLQIHCTRYLMRSQNGGSTDLPR
                     YWITLDKNVIWDYPKDFIAGNAGVRNFHGETCWYPYLTDICSISDLLREYIDTPKAEL
                     LTKQFTSDKWGLVNILRAADRRIGMRRLDQLRRKTHNIAALKIIARRSE"
     gene            complement(37917..38252)
                     /locus_tag="JNC24_001023"
                     /pseudo
     CDS             complement(37917..38252)
                     /locus_tag="JNC24_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
BASE COUNT         9473 a        10314 c         9728 g         8822 t
ORIGIN      
        1 tgtttaccca acatgcgctc cgcactacac tatgtgggca tttttttaag gggtggtaat
       61 gaacgattat aaaatgacgc caggtgagag gcgcgcgacc tggggtttag ggaccgtatt
      121 ctcgttgcgc atgctgggca tgtttatggt tctgccggtt ctgaccacgt acggcatggc
      181 tctgcaaggt gccagcgaag cattaatcgg tattgccatt ggtatttatg gtctgactca
      241 ggccgttttt cagattccgt ttggcctgct ttccgaccgc attggtcgca aaccattaat
      301 tgtcggtggg ctggcggtgt ttgccgccgg tagcgttatc gctgcgcttt ccgactccat
      361 ctggggaatt attctgggcc gggcgctaca aggctctgga gcaattgccg ccgccgttat
      421 ggcgctgctt tccgatctca cgcgcgaaca aaaccgcacc aaagcgatgg cgtttatcgg
      481 cgtgagcttt ggcattacct ttgccattgc gatggtgctt ggcccgatca tcactcacaa
      541 acttgggctg cacgcgctgt tctggatgat cgctattctg gcaacaaccg gcattgcgtt
      601 gaccatttgg gttgtgccca acagtagcac tcacgtactt aatcgtgagt ccggaatggt
      661 gaaaggcagt ttcagtaaag tgctggcgga accgcggctg ctgaaactca actttggcat
      721 tatgtgtctg cacatgcttc tgatgtctac gtttgttgcc ctgcccggac agctagctga
      781 tgcggggttc ccggcggctg aacactggaa ggtctatctg gcgacgatgt taatcgcctt
      841 tggctcgatc gtgcctttca ttatctacgc tgaagttaag cgcaaaatga agcaagtctt
      901 tgtcttctgc gtcgggttga tcgtggttgc ggaaattgtg ttgtggaacg cacaaacgca
      961 gttctggcaa ctggtggtcg gcgtgcagct tttctttgtg gcgtttaatt tgatggaagc
     1021 ccttctgccc tcacttatca gtaaagagtc gccagcaggt tacaaaggta cggcgatggg
     1081 tgtctactcc accagccagt ttcttggcgt ggcgattggc ggttcgctgg gcggctggat
     1141 tgacggcatg tttgacggtc agggggtatt tctcgctggc gcaatgctgg ccgcagtgtg
     1201 gctggcagtc gccagtacca tgaaagaacc gccgtatgtc agcagtttgc gcattgaaat
     1261 cccggcggac attgccgcaa acgaagcgtt aaaagtgcgt ttgctggaaa ctgaaggcgt
     1321 caaagaagtg ttgattgcag aagaagagca ttcagcttat gtgaaaatcg acagcaaagt
     1381 gacaaatcgc tttgaggttg aacaggcaat tcgtcaggca taaaaaacgc ggcgaattgc
     1441 ctgctgcgct acgcttacca ggcctacaaa gttcttgagt tacaaaccgt tgtaggccga
     1501 atcaggcgtt catgccgcat ctgacatgaa caaaacgcac attgtcgcga ttaatcgcgg
     1561 aagtttttga actggaacgg ctggccgaga tcgccaccac gtaccatcgc catgacagcc
     1621 tgcaaatcat cacgagattt gccagtcaca cggatctcat cgccctgaat ttgcgcctgc
     1681 actttcagtt tgctgtcttt aatcatcttg acgattttct tctgggtcgc gctctcaatg
     1741 ccctgtttca gtttcgcttc cacaaaccag gttttaccgc tatgaacgat attttccggt
     1801 acatccagtg aactgccttc aatgccgcgc ttcagcagct tggcacgcag aatatccagc
     1861 aactgattga cctggaaatc ggactcgctc aacactttga tggttttgct ggcgtcgttc
     1921 agctcaaatg aggcttcaac attacggaag tcaaaacggg actccacttc gcggctcgcg
     1981 ttatcgaccg cgttacgtgc ttcctgaaga tcaacttcag agacaatatc gaaagatggc
     2041 atcttttcct ctcccttcat ttttgatgcg aagcataata cccgcaaagt taaataaccg
     2101 acagtcctgc accaggacgt atcccttaat tgtgcatagt cacaacagca tgcacaatta
     2161 agggatagcc ttattgaaca cccggagtgg ttgcgggtga ggaggaacaa tgaaaattac
     2221 cgtattggga tgcggtgcct tagggcaatt atggcttaca gcactttgta aacagggtca
     2281 cgaagttcag ggctggctgc gcgtaccgca accttattgt agtgtgaatc tggttgagac
     2341 tgatggttcg atatttaacg aatcgctgac cgccaacgat cccgattttc ttgccaccag
     2401 cgatctgctc ctggtgacgc tgaaagcatg gcaggtttcc gatgccgtca aaagcctcgc
     2461 gtccacactg cctgtaacca cgccaatact gttaattcac aacggcatgg gcaccatcga
     2521 agagttgcaa aacattcagc agccattact gatgggcacc accacccatg ccgcccgccg
     2581 cgacggcaat gtcattattc atgtggcaaa cggaatcacg catattggtc cggcacggca
     2641 acaggacggc gattacagct atctggcgga tattttgcaa accgtgttgc ctgacgttgc
     2701 ctggcataac aatattcgcg ccgagctgtg gcgcaagctg gccgtcaact gtgtgattaa
     2761 tccactgacc gccatctgga attgcccgaa tggtgaatta cgtcatcatc cgcaagaaat
     2821 tatgcagata tgcgaagaag tcgcggcagt gatcgagcgc gaagggcatc atacttcagc
     2881 agaagatttg cgtgattacg tgatgcaggt gattgatgcc acagcggaaa atatctcgtc
     2941 gatgttgcag gatatccgcg cgctgcgcca caccgaaatc gactatatca atggttttct
     3001 cttacgccgc gcccgcgcgc atgggattgc cgtaccggaa aacacccgcc tgtttgaaat
     3061 ggtaaaaaga aaggagagtg aatatgagcg catcggcact ggtttgcctc gcccctggta
     3121 gtgaagagac tgaagccgtc accactatcg atctgctggt tcgcggcggt atcaaagtca
     3181 ccactgccag cgtcgccagc gatggcaacc tggccattac ctgctcgcgc ggcgtgaagc
     3241 tgctggcgga tgcgccgctg gtcgaggtgg ctgatggcga atatgacgtg atcgtactgc
     3301 ctggtggcat taaaggcgca gagtgttttc gcgatagtac cctgctggtt gaaaccgtta
     3361 aacagttcca ccgttccggg cgtatcgtcg cggctatttg cgccgcgcca gccaccgtgc
     3421 tggtaccgca cgatatcttc ccgattggca atatgaccgg cttcccgacg ctgaaagaca
     3481 aaattcccgc cgaacaatgg caggacaagc gcgtcgtctg ggatgcacgg gtaaaattgc
     3541 tgaccagcca ggggccgggt acggctattg attttggtct gaaaattatc gacctgctgg
     3601 ttgggcgcga gaaaacccat gaagtggcat cacaactggt gatggcggca gggatttata
     3661 attattacga gtagtgtcgg atgcggcaaa cgtcgcatct gaccagatgc gacgtaaaaa
     3721 ccacaaatta tgggcgatac accttcacat tgttaaagcc ctgctcgcgc agatagagcg
     3781 cctgcagacg gctcatcacc ccgcgctcac accacagtag ccaggttctg ttctggtcga
     3841 gatcgccaaa tttggtgctc agtttataga acggcagaga aaccacgtcg ataccttcaa
     3901 ccttcagcgg cttatcttcc tgctcatcga cagaacggat atcgaggatc acgtcgttcg
     3961 ggccaaagcc attgacggtt tccacttcca ccacttcctg ctcggtctgc tgggcgattt
     4021 cgcggatatc aacgttattc gcttcctcaa ccactttatc gagaatactg aagtcgaact
     4081 tctcttcttc cgcttcaatc ttcgatttaa ccgctttcac cgtcgggctt ttggagatca
     4141 cgccacaata ttctggcatc gtgcgagcaa agtcttcagt gccaatctgg cgggccaggt
     4201 tgatgatgtg ctctttgtcg taagaaatca gcggacgcag gatcagcgta tcagagacgt
     4261 tatcaatcag gcgcaggttg gtcagcgtct gactggacac ctggccgagc gcttcgccgg
     4321 tgaccagcgc ctgtacgccg taacgttcag ccactttaga cgcggcacgc accatcatac
     4381 gtttgaggat aacgcccatc tgaccgtcgt cgattttctc gagaatttcc ccgacgaccg
     4441 gttcgaaatt aatggcgaca aaacgcacgc ggtgggagct gccaaagcgg ttccacagat
     4501 aatgcgccac ctgacgcacg ccaatttcat gcgctgcgcc gccgaggtta aagaagcagt
     4561 aatgcacgcg gcagccgcga cgcatcaaca tataactgga aacaccggag tcgaaaccac
     4621 cggaaatgag cgacagcaca tcttcctggg tgccaatcgg gaaaccgcca ataccttcgt
     4681 agcggccttt aatcagcaga agacgatcgt cttccacttc cagatggaca gttacatccg
     4741 ggttagtcag cttcacgcgc gcggattcaa tatgctgatt taaaccgccg ccgacgtaac
     4801 gttccacatc aatcgagcta aaatcatgtt tgccacggcg cttcacgcgt acgcagaagg
     4861 ttttgccttc cagctgatcg cgatattgaa ccaacgcttt ctcgaaaata tcgtgcatgt
     4921 cggtaaacgg cacgtcttcg acttcgagaa tatggtggat acccggaata cgggtcaggg
     4981 cgtcgcgaat agccagacgc tgtttttcat cttttgcgcg aacttcgatg ttatcccagt
     5041 ggcggacgac agcgagcgtc tcatcatagt gctttaaaac gttacgaatg ttcccggtaa
     5101 ggatttttat aaagcgcaag cgcacagatt ggcttttgat ggtgatttcc gggaacaatt
     5161 taatgataaa cttcatggcg gcaatggttc gttggcaagc cttaagcgac ttgtataggg
     5221 aaaaatacag cagcccacac ctgcggctgc atccaggcgc ggaagtatac cactaacatc
     5281 gctttgctgc gcacatcacc ttaccattgc gcgttatttg ctatttgccc tgagtccgtt
     5341 accatgacgg ggcgaaaaat attgagagtc agacattcat tatgccgaag aaaaatgagg
     5401 cgcccgccag ctttgaaaag gcgctgagcg agctggaaca gattgtaacc cgtctggaaa
     5461 gtggcgacct gccgctggaa gaggcgctga acgagttcga acgcggcgtg cagctggcac
     5521 gtcaggggca ggccaaatta caacaagccg aacagcgcgt acaaattctg ctgtctgaca
     5581 atgaagacac ctctctaccc ccttttacac cggacaatga gtaatggact ttccgcagca
     5641 acttgaagcc tgcgttaagc aggccaacca ggcgctgagc cgttttatcg ccccactgcc
     5701 ctttcagaac actcccgtgg tcgaaaccat gcagtatggc gcattattag gtggtaagcg
     5761 cctgcgacct tttctggttt atgccaccgg tcatatgttc ggcgttagca caaacacgct
     5821 ggacgcaccc gctgccgccg ttgagtgtat ccacgcttac tcattaattc atgatgattt
     5881 accggcgatg gatgatgacg atctgcgtcg cggtttgccg acctgccatg tgaagtttgg
     5941 cgaagcaaac gcgattctcg ctggcgacgc tttacaaacg ctggcgttct cgattttaag
     6001 cgatgccgat atgccggaag tgtcggaccg cgacagaatt tcgatgattt cagaactggc
     6061 aagcgccagc ggtattgccg gaatgtgcgg tggtcaggca ttagatttag atgcggaagg
     6121 caaacacgta cctctggacg cactcgagcg tattcatcgt cataaaaccg gcgcattgat
     6181 tcgcgccgcc gttcgccttg gtgcattaag cgccggagat aaagggcgtc gtgctctgcc
     6241 agtactcgac aagtacgcag agagcatcgg ccttgccttc caggttcagg atgacatcct
     6301 ggatgtggtg ggagatactg caacgttggg aaaacgccag ggtgccgacc agcaacttgg
     6361 taaaagtacc taccctgcac ttctgggtct tgagcaagcc cggaagaaag cccgggatct
     6421 gatcgacgat gcccgccagt cgctgaaaca actggctgaa caatcactcg atacctcggc
     6481 actggaagcg ctagcggact acatcatcca gcgtaataaa taaacaataa gtattaatag
     6541 gcccctgatg agttttgata ttgccaaata cccgaccctg gcactggtcg actccaccca
     6601 ggagttacga ctgttgccga aagagagttt accgaaactc tgcgacgaac tgcgccgcta
     6661 tttactcgac agcgtgagcc gttccagcgg gcacttcgcc tccgggctgg gcacggtcga
     6721 actgaccgtg gcgctgcact atgtctacaa caccccgttt gaccaattga tttgggatgt
     6781 ggggcatcag gcttatccgc ataaaatttt gaccggacgc cgcgacaaaa tcggcaccat
     6841 ccgtcagaaa ggcggtctgc atccgttccc gtggcgcggc gaaagcgaat atgacgtatt
     6901 aagcgtcggg cattcatcaa cctccatcag tgccggaatt ggtattgcgg ttactgccga
     6961 gaaagaaggc aaaaatcgcc gcaccgtctg tgtcattggc gatggcgcga ttaccgctgg
     7021 catggcgttt gaagcgatga atcacgcggg cgatatccgt cctgatatgc tggtggtcct
     7081 caacgacaat gaaatgtcga tttccgaaaa tgtcggcgcg ctcaataacc atctggcaca
     7141 gctgctttcc ggtaagcttt actcttcgct gcgcgaaggc gggaaaaaag ttttctctgg
     7201 cgttccgcca attaaagagc tgctcaaacg taccgaagaa catattaaag gcatggtagt
     7261 gcctggcacg ttgtttgaag agctgggctt taactacatc ggcccggttg acggtcacga
     7321 tgtgctgggg cttatcacca cgctgaagaa catgcgcgac ctgaaaggcc cgcagttcct
     7381 gcatatcatg accaaaaaag gtcgtggtta tgaaccggca gaaaaagacc ccatcacttt
     7441 ccacgccgtg cctaaatttg atccctccag cggttgtttg ccgaaaagta gcggcggttt
     7501 gccgagctat tcaaaaatct ttggcgactg gttgtgcgaa acggcagcga aagacaacaa
     7561 gctgatggcg attactccgg cgatgcgtga aggttccggc atggtcgagt tttcacgtaa
     7621 attcccggat cgttacttcg acgtggcaat cgccgagcaa cacgcggtga cctttgccgc
     7681 cggtctggcg attggtgggt acaaacccat tgtcgcgatt tactccactt tcctgcaacg
     7741 cgcctatgat caggtgctgc atgacgtggc gattcaaaag ctcccggtcc tgttcgccat
     7801 cgaccgcgcg ggcattgttg gtgctgacgg tcaaacccat cagggcgctt ttgacctctc
     7861 ttacctgcgc tgcataccgg aaatggtcat tatgaccccg agcgatgaaa acgaatgtcg
     7921 ccagatgctc tataccggct atcactataa cgacggcccg tccgcggtgc gctacccgcg
     7981 cggtaacgcg gttggcgtgg aactgacgcc gctggaaaaa ctgccaattg gcaaaggcat
     8041 tgtgaagcgt cgtggcgaga aactggcgat ccttaacttt ggtacgctga tgccagacgc
     8101 ggcgaaagtc gctgaatcgc tgaacgctac gctggtcgat atgcgttttg tgaaaccgct
     8161 tgatgaagcg ttaattctgg aaatggccgc cagccatgaa gcgctggtca ccgtagaaga
     8221 aaacgccatt atgggcggcg caggcagcgg cgtgaacgaa gtgctaatgg cccatcgtaa
     8281 accagtaccc gtgctgaaca ttggcctgcc tgacttcttt attccacaag gaactcagga
     8341 agaaatgcgc gccgaactcg gcctcgatgc cgccggtatg gaagccaaaa tcaaggcctg
     8401 gctggcataa tccctactcc actcctgcta tgcttaagaa attattcatg gcaggagtgg
     8461 cagcatgcaa tacaacccct taggaaaaac cgaccttcgc gtttcccgac tttgcctcgg
     8521 ctgtatgacc tttggcgagc cagatcgtgg taatcacgca tggacactac cggaagagag
     8581 tagccgtccc atcattaaac gcgcgctgga aggcggcata aatttctttg ataccgccaa
     8641 tagctattcc gatggcagca gcgaagagat cgttggtcgc gcactgcggg atttcgcccg
     8701 tcgtgaagac gtggtcgttg cgacgaaagt gttccatcgc gttggtgatt taccggaagg
     8761 attatcccgt gcacaaattt tgcgctctat caacgacagc ctgcgccgtc tcggcatgga
     8821 ttatgtcgat atcctgcaaa ttcatcgctg ggattacaac acgccgatcg aagagacgct
     8881 ggaagccctg aacgacgtgg taaaagccgg gaaagcgcgt tatatcggcg cgtcatccat
     8941 gcacgcttcg cagtttgctc aggccctgaa gctacaaaaa cagcacggct gggcgcagtt
     9001 tatcagtatg caggatcact acaatctgat ttatcgcgaa gaagagcgcg agatgctgcc
     9061 tctgtgttat caggagggcg tggcggtgat tccgtggagc ccgctggcgc gggggcgact
     9121 gacgcgtccg tggggagaaa ctaccgcacg actggtgtct gatgaggtgg ggaaaaatct
     9181 ctataaagaa agcgatgaaa atgacgcgca gatcgcagag cggttaacgg gcgtcagtga
     9241 agaactcggt gcaacacgag cacaagttgc gctggcctgg ttattgagta aaccgggcat
     9301 tgccgcaccg attatcggta catcgcggga agaacagctt gatgagctgc tgaacgcggt
     9361 ggatatcact ttgaagccag aacagattgc cgaactggaa acgccgtata aaccgcatgc
     9421 ggtcgtagga tttaaataag agtccaggcc tgatgagacg tgacaagcgt cacatcaggc
     9481 atcggtgcac aactacgaca gaatacccag cggccagtga tgaccgataa agtacaggat
     9541 gcctgcggaa atcaccccgg cgacaatatc gtcgatcatg atccccatgc cgccatgtac
     9601 attgcgatca aaccagcgga tcggccacgg cttccacata tccagaatac ggaaaatcac
     9661 aaacccggcg gcaacccact gccagtcatt ggtcggcagc gccatgagcg tgatccacat
     9721 accaataaat tcgtcccaga caatgctgcc gtgatcgtgc acgcccatat ctttcgccgt
     9781 ttgatgacaa agatagacgc cgatacagat ccccaacatc accaccagcg agtagagctg
     9841 ccagggtaaa aaggtcatca gataccagaa cggaatcgct gccagcgatc ccatcgtgcc
     9901 aggaacgatt gggcttaatc cactcccgaa tccgacagca agcagatgcc acggattact
     9961 catcttcagg cgacttttcg cgacatcttt atggcgtggc aaaatggtca tatcctttcc
    10021 agtcaaacgt gacaggttcg ccgtcacgaa taaaacaaag cccttcgata tcggcggtca
    10081 tttgcccgat acaggtaaac ggtacgccaa ggtgtccgag agcaacatcc agcgcgccac
    10141 ggttcagttc cggcaccgtg aaacacaact cgtaatcttc accgccagag agcgcccagc
    10201 gcagcgcctg ttccggttca acatggcgag aaagcgcatc agaaaacggc agcaatgcca
    10261 ggtcaatacg tgcgccgcag tcgctggctt tcacgatatg cccgagatcg gaaatcagac
    10321 cgtcagagag atcgatagct gaatttgcca gatcgcgcag tgcctgcccc tgtaaaatac
    10381 gcggcgatgg acggagatga cgtttgatca agtagtcagc atctttagca tcggcaacct
    10441 gcaaacggtt ttgcaaaatc gccagcccgg cggcgctatc gcccggtgta ccggtcacat
    10501 agatccagtc acccggtttc gccccagagc gcgttaaggc tcgtcccatc ggaacaaagc
    10561 cgtggatacc caacgtcatt gataatggcc cacgcgtggt atcgccgcca atgagttgca
    10621 tatcgtaata attgagaaga tcaaacaaac tgtcgctgaa ggactcaagc cacgcttcgt
    10681 ctacgtccgg taaggttaat gccagcgtca gccaggccgg atcggccccc atcgctgcca
    10741 gatcgcttag gttcaccgcc agtgctttat aagccagatc agcaggatcg atatcaggga
    10801 ggaaatggtt acccgccacc agcgtatcag tgctgatcgc cagggtctgt ttctcgggga
    10861 tattgagaag tgcgcaatcg tcgccgatgc ccagttcgac atcaagacga gaacttctta
    10921 cacggtcaaa ataacgggca atcagggaga actcgccaca tgccatacgt tatgcctcag
    10981 caggtaaaaa agaaaaggcc gacgatcatg gaacggtctt ccgtgaatct accggcctgg
    11041 atatcacttt ttgttagggc gaatcacagg tgctgcttta tcgagtacgc cgttgacgaa
    11101 cttatggctg tcttctgcgc cgaacgattt cgccagttcg atcgcttcgt taatggccac
    11161 tttgtatggc acatcgctac gtttagacag ttcgtacagc gcaatgcgca gtactgcttt
    11221 ttctacctgt cccagttctt ccagcaggcg ggacaggtat ggcttcatca gtccgtcgag
    11281 gtatgcggta ttagtcgcca ccccggccag cagctcacgg aagtacagga cgtcaacgtc
    11341 ttttacatcc tgttcagcca ggaactggta ttcaacatca gcgatgtcgt tctgggacaa
    11401 ctgccaggag tagagcgcct ggacggcaca ctcacgagcg cggcgacgag cagcaggttt
    11461 cacggatttc cccttactaa tttcaggcct tgatggcttt caatacatta atcatttcaa
    11521 gcgcggtcag tgcagcttct gcacctttgt tgccagcttt ggtgccagca cgttcgatcg
    11581 cttgttcaat gctttcagtg gtcagaaccc caaaagcaac cggaatttcg ctgtcctggg
    11641 caacatgcgc caggccgttg cttgcaccac cagcgacata ttcaaagtgg gcagtgccac
    11701 cacgaataac cgtacccagc gcaatcaccg cgtcgtattt accggtttta gccagtgcac
    11761 ccgccgccag cggcagctca taggcaccag gcacccaaac aacggtaatg ttttcatctt
    11821 ttacctgacc gatacgtttc agtgcgtcaa ttgcaccttc cagcaggctg tcattgataa
    11881 agttgttgaa acgcgcgatg gtgatggcga cgcgagcgtc cggggtagca acgttagctt
    11941 caataatgtt cattttcttc cttcgggttc gagtatggcc ccgcaggggg gcggatttta
    12001 gcataatatt tcgtgcgctg cttccctttc gagccgggag atcatgcacc cactaaatgc
    12061 aggcaaacat ccgggcctac atgacgtatc tctttgaatt taaattgggg ggcgtcggct
    12121 aatttctcaa gccctggcag cgtgcataat ccgcgggcgt cgctgcctaa tagtttaggt
    12181 gcgatataga caatcagctc atcgactaaa cccgcctgca gcaatgcgcc agcgagcgtt
    12241 ggccccgctt ccacccagat gctgttaatt tgctgtttac ccagttgcat catcagtaca
    12301 accagatcca gatgaccttt atgctctgga atcagcaagg tacgcaccgt ttccggccac
    12361 tcactagaat cttcctgcgt acgcgcgaac caggtttcgc cgggctgctg cacaatgcga
    12421 tgttccggcg tcacgcgatt ttggctatca atcacaatac gtactggctg gcggagattt
    12481 tgttgcgggt agagcgcctg agtttgttca tccagttcag accaacgcac tgttaaggct
    12541 ggatcatccg ccagcaccgt ggcgctgctg gttaaaatgg catgactttg tgcgcgctgt
    12601 cgttgtacgt cgcgccgcgc ctgaggcgaa gtgatccact ggctttcgcc gctcgccatc
    12661 gccgtgcggc catcaagcga tgcgccaagt tttaactgaa tataaggaaa gccggtgcgc
    12721 atccgcttga gaaagccttt attcaattgc tcggcttcac tcatcatcag cccgtggctg
    12781 acgtcaatgc cagcctgttg cagacggtaa agtccacgcc cagcgacctg cgggttagga
    12841 tcctgcatcg ctgcgaccac gcgcgctacg ccagcggcga tgagtgcgtc acagcacggt
    12901 ggcgtacggc catgatggct acagggttcg agtgtcacat acgccgttgc gcctttggct
    12961 ttttcacccg ccatacgcaa cgcgtgtact tcggcatgtg gttcacccgc tcgttggtgg
    13021 taaccttcac cgacaatttc gccatctttg acaatgacgc ccccgacatt cgggttgggg
    13081 tgggtggtaa aacgtcctcg ttgcgccagc tttagcgccc gcgccatgta atactcgtcc
    13141 tgcacggctt agtcctccag gcgcgcgatc tcttcgccaa attctttgat atcttcgaaa
    13201 ctgcggtaga cagaggcaaa acggatatag gcgactttat cgagcttttt caattgctcc
    13261 atcaccagat tgccaatcat cttgctcggt acttcgcgct caccggtggc gcgcagctgc
    13321 gatttaatat gattgattgc catttcaacg tcatcggaac tcaccggacg tttttccagc
    13381 gcccgcagca ttccgctacg caatttctct tcattaaacg gttcacgcac gtcgttgctt
    13441 tttacaacac gcggcataac cagctccgcc acttcaaagg tggtgaaacg ttcattacac
    13501 accagacact gccgacggcg gcgtacggat gaaccctcgc ccacgagacg agagtcaatt
    13561 accttagtgt ccacggcgaa acagaatggg caatgcatac ggcgtcctga ccaggtggtt
    13621 aacggaaatc tattttaccc tgaactgacg atacaacaaa ggcgcaacgc ttttgagata
    13681 aaggatcgat gctttcgctg cttttaccct ctaccattag gctaattgcg acgagaaagg
    13741 gacgacctat gaatactaac gtttttcgac tgctcctgct gggaagcctg ttcagcctta
    13801 gcgcctgtgt gcagcaaagt gaagtgcgac agatgaaaca cagcgtcagc acgctgaacc
    13861 aggagatgac gcaactcaat caagaaacag tcaaaatcac tcagcaaaac aggctgaatg
    13921 caaaatccag cagtggggtt taccttctgc ctggagcgaa aacaccggca agactggaaa
    13981 gccagatcgg tactttacgt atgtcgctgg tgaatattac gcctgataca gatggcacca
    14041 cccttacact acgtattcag ggtgagtcga atgatccctt gcccgcattc agcggaactg
    14101 ttgaatatgg gcaaattcag ggaacaatag acaactttca ggaaatcaat gtgcagaatc
    14161 aattgattaa tgcccctgcc agcgtcctcg cccccagcga tgttgatatt ccgttacaat
    14221 taaaggggat ttctgtggat cagttaggct ttgtacgtat ccacgacatt caacctgtta
    14281 tgcagtaaac gtatttcggg acgattttgt gcgtcccgca acatctttcc ccgtcatttt
    14341 gttactctgc ttacatcacc tggattgata gtaaaagttt gcaacaaggg cgaaagtcag
    14401 tacaatcccc gcccgaatgt gtgtaaacgt gaacgcaatc gattacgtaa atgatagaac
    14461 tgtgaaacga aacatatttt tgtgagcaat gatttttata ataggctcct ctgtatacga
    14521 aatatttaga aacgcaattt gcgccttttt cactcccgca agggattttc aaacagtggc
    14581 atacatatga aaaaaacatt actggcagcc ggtgcggtac tggcgctctc ttcgtctttt
    14641 actgtcaacg cagctgaaaa cgacaaaccg cagtatcttt ccgactggtg gcaccagagc
    14701 gttaacgttg tcggaagcta tcacacccgt ttcggaccgc agatccgcaa cgatacctac
    14761 cttgagtacg aagcattcgc taaaaaagac tggttcgact tctatggtta tgcggatgcg
    14821 ccggtattct tcggcggtaa ctccgatgct aaaggtatct ggaaccacgg ttctccgctg
    14881 tttatggaaa tcgaaccacg tttctccatc gacaagctga ccaatactga ccttagcttc
    14941 ggtccgttca aagagtggta cttcgcgaac aactacattt acgacatggg tcgtaataaa
    15001 gatggtcgcc agagcacctg gtacatgggt ctgggtaccg acatcgatac tggtctgccg
    15061 atgagcctgt ccatgaacgt ctatgcgaaa taccagtggc agaactatga cgcagcgaac
    15121 gaaaacgagt gggacggtta ccgtttcaaa attaaatact ttgtgccgat taccgatctg
    15181 tggggcggtc agctgagcta catcggcttc accaacttcg actggggttc cgatttaggg
    15241 gatgacagcg gtaacgcaat caacggtatt aagacccgta ctaataactc tatcgcttcc
    15301 agccatattc tggctctgaa ctacgatcac tggcactact ctgtcgtagc tcgttactgg
    15361 cacgacggtg gtcagtggaa cgacgatgca gaactgaact tcggcaacgg caacttcaac
    15421 gttcgctcta ccggctgggg tggttacctg gtagtaggtt acaacttctg attatgaaaa
    15481 tgccgggatt tattcccggc atttctgatt gttaacaacg tgtgctttgt tcatgccgga
    15541 tgcggcgtaa acgctttgtc cggcccacaa aagcacgtaa attcaatata ttgctgaaat
    15601 aacgtaggcc tgataagcgt agcgcatcag gcaattttac gttttaatca cttcttcttg
    15661 ttcatcatcg ctttcaggtc agcgaatggg ttgtacttcg cttcaccgac atctttctgc
    15721 gcatcttccc ctgcgataac ggtcgtacca tactgatccg cttcggtata tttcgaatgc
    15781 tcatgatcgt ggcaatagag acaaagcaat tcccagttac tgccatcttc cgggttattg
    15841 gtatggtcgt gatcaatgtg gtgaacggta agttcacgca ggttggaata aacaaactcg
    15901 cgggaacagc gaccgcagac ccacggatag atttttaatg ctttttcgcg atagccgctt
    15961 tctaaccgcg catagttttt tgggatgata gccatgaaaa gttacctgga tgaaatgtag
    16021 ggttacgcgc agtttcccaa gatgcgggga ttttcgcaat ctccatacat caaaaaaaat
    16081 cccgatcttc tgaccgggat tttcaacatc aacgggaact tgattacggc agaattgacg
    16141 gctgatccgc cccttctttt tccactttct gctgcaacat gtgttcgcgc ttcataccca
    16201 gtttcagagc caacgcagat gctacgtaga tggaagatgc agtgccgatg gaaacaccga
    16261 taagcatggt cagcgagaag ccttccagta ccggaccacc gaagagatac agcatcagga
    16321 taaccatcaa ggtagtaccg gatgtgatca aggtacggtg cagcgtctgg gtcaaggaca
    16381 cgttaaagat ttcgtaaggc gtaccgcgac ggatcttgcg gaagttttca cgaattcggt
    16441 ccgataccac gatactgtcg ttaagcgagt aaccgataac cgacatcaac gatgccacaa
    16501 tggtcaggtc aatctcgata tggaataacg acaaaatacc cagcgtaatg ataacgtcgt
    16561 gcgccagcgc aataaccacc cctgccgcca gtcgccactc aaagcggaaa cctacgtaca
    16621 cgaggataga cagcagcgct gccatcaacg ccatcgcacc ggtttgcgca aggtctgccc
    16681 ccacgctcgg accgacgaac tcaatacgct tcactgctgc attctgattg gtggattcgt
    16741 taatcacctt cagaacctgg ctgcccagca cctgaccgcc ggtttcgcct tcagcaggcg
    16801 gcatacggac catgatgtcg tggctgctac caaagttttg cagcattggc tcttcaaaac
    16861 cggctttttg caatgcatca cgcattacgt caatttcagc cggtttttcg agcgtaattt
    16921 cgataaccgt accaccggtg aaatccagcc cccagttaaa gccgcgcacg cccataataa
    16981 cgatagcagc gattaacagc agaccagaga tgccgaaagc ccagtagtcc cagcgcataa
    17041 agtcatagac tttacggccg tggtttagtt gttcaacagt atattcctgt gccacatcgc
    17101 actcctcaga ttgacagctt cttgacgcgc ttgccgccat atagcaggtt tacgatggca
    17161 cgcgtaccga caatcgcggt aaacatcgac gtcgccacac cgataccggt agtaatcgcg
    17221 aaccctttaa ttgccccggt acccactgcg tacaggatga tgactttaat cagcgtggtg
    17281 atgttcgcat cgaagataga actgaatgcg ccacgataac cttcatcaat tgcctgttga
    17341 acagtacgtc cgttgctcaa ctcttcttta atacgttcgt tgatcagtac gttcgcatcg
    17401 accgctaccg caagggttaa gacgataccc gcgatacctg gcatactcag tgttgcgcct
    17461 ggcaacagcg acataatacc gacgatcaag atcaagttgg caatcagagc actggtcgca
    17521 atcagaccaa acttcttata gaagatgatc atgaacagaa tagaaaccag cagaccggca
    17581 aggcaagcct ccagcccctg ctcaatgttc tgcataccca gggtcgggcc aattgtacgt
    17641 tcttcaacaa tctgaatcgg cgcgatcaac gcaccggcac gcagcagcag tgacagctga
    17701 cgggcttcgt tcgggttgtt gatgccggtg atacggaagc tgttacccag acgagactgg
    17761 atgttggcga tgttaatcac ctcttcctgt ttcaccagaa ccgcacgacc atttgcatct
    17821 ttcttaccgc tgtctttgta ctccacaaac agggttgcca tcggtttgcc gatgttgtcc
    17881 ttggtgaagt tagacataat gttgccacca gcgctatcga gcgagatgtt aacctgcggc
    17941 tggttgtatt cgtcctggct ggaagtggag tcggtgatat ggtcaccggt cagaattacg
    18001 cgtttgtaca gcacaaccgg ctgaccttcg cgggtctgtt tcacttcaga gtcgcccggt
    18061 acgcgaccgg atgccgcagc ggcctgatca acattggtgt ttaccagacg gaattccagc
    18121 gttgccgtcg cgcccagaat ctctttcgca cgcgcagtgt cctgaatacc aggcagttca
    18181 acaacgatac ggtcagcacc ctgacgctga accaccggct ccgccacgcc aagttggttt
    18241 acacggttac gcaggatatt aatgttctgc tgcaccgcat attcacgcgc ttcactcaga
    18301 cgagcatcgc tcattaccgc acgcagctgg ttgctgccct ggctgctaat caccaggtcc
    18361 ggatggcgct tgctcagata cgcaatggct tcatcacgag ctttagcatc gcggaaagtg
    18421 atgctcaggc cgtagttgtt ttctttacga acagtggtat acgggatgcc cttttcgcgc
    18481 aggtcactgc gtaggctatc gatattttgt tcctggagtt tgccaagcgc ggtgtccata
    18541 tccacttcca tcaggaagtg aacgccgcca cgcaggtcaa ggccgagctt catcggctca
    18601 gcatgaatag ctgccagcca gcgcggcgtt gccggggcaa ggttaagcgc cacgacgtat
    18661 ttgtcaccca taacgcccat taatgcttca cgagcgcgca actgggtgtc agtggagtcg
    18721 aagcgcgcaa gaatagcgcc ctcttccagt gccacagact tagcagttat tttttcttct
    18781 tgtaacgttt tctggacctg gatcagcgtt tgctcactgg cggcgactcc gcgcgcacca
    18841 gtgatctgaa cagccggatc ctcaccaaac aggttgggaa gcgcatacag cagaccgatg
    18901 acaatcacca cgatcagcat gacgtacttc cacaaaggat aacggtttaa cacggcaatt
    18961 cccttaggga aaaattttaa ttacagcgcc ttcatggtgc ctttcggcag gacggcagct
    19021 acgaagtcac gtttaataac tacttcagtg gtgtcattca gcgcgatagc aatgtagccg
    19081 ttttccgcta ctttggttac gcgaccaacc aggccaccgt tcgtcagaac ttcatcacct
    19141 ttggcaatgg agtccatcag ctttttgtgt tctttggtgc gcttctgctg tggacgcagg
    19201 atcatgaaat agaaaatcag accgaatacc accagcatca aaatcaaaga catcgggcta
    19261 ccttgcgccg gtgcacccgt tgccgctacc gcatcagaaa taaaaaagct cattaaattt
    19321 ccctcattat taatattaat caacgttcaa aggtggtact tctcgcccct gacgctggta
    19381 aaaatcagtt acgaagctct ctaatttacc ctcttcaata gccttgcgta aacccgccat
    19441 caaacgctgg tagtaacgaa ggttatgaat ggtgttgagt cgcgcgccta atatttcgtt
    19501 gcaacggtca agatgatgca agtaagcgcg tgaataattg cgacaggtgt agcaatcaca
    19561 ctcaggatca agtgggccag tatcgctctt atacttcgca ttgcggattt tcaccacgcc
    19621 atcggtcacg aacaaatgac catttcgggc gttgcgggtt ggcattacgc agtcaaacat
    19681 atcgatacca cgacgtacgc cttcaaccag gtcttctggt ttaccaacgc ccatcaggta
    19741 acgcggtttg tctgccggaa tttgcgggca tacatgctcc agaatgcggt gcatatctgc
    19801 tttcggctca cccacagcca gaccgccgac agcgtagcca tcaaaaccga tatctaccag
    19861 acctttaaca gaaatatcac gtaaatcttc gtaaacgctg ccctgaatga taccaaacag
    19921 cgcatttttg tttccgagac tgtcaaaacg ctcacggcta cgcttcgccc aacgcagaga
    19981 catctccatg gagcgttttg cgtaatccca gtcagcagga tacggcgtac actcatcaaa
    20041 gatcatgacg atatccgaac caagatcgta ctgaatctcc attgattttt caggatcgag
    20101 gaaaatcgga tcgccgttga tcgggttacg gaagtgcacg ccctgttcgg tgattttacg
    20161 aatatcgcca aggctgaaga cctggaagcc gccggagtcg gtgaggatcg gccccttcca
    20221 ctgcataaaa tcgtgcagat cgccgtgcag tttcatgatt tcctggcccg ggcgcagcca
    20281 caggtggaag gtgttgccga ggataatttg cgcgccagtg gcttcaactt cttccggcgt
    20341 catccctttt acggtgccgt aggtgccaac aggcataaaa caaggcgttt ccactacgcc
    20401 acgatcaaag accaggcggc cacggcgtgc gcgaccgtcg gtggtgtcca gttcaaattt
    20461 cattttttct ccactacgtc agaaaaacag tccaacgttt taaaccagcg ccacggaatt
    20521 actccccgac gcgctcatta attgcctgcg gattgtacgt gataaacatc gcatcaccgt
    20581 aactaaaaaa gcgatatttc tcttctaccg ctgctttata ggcgttcatg gtgtgttgat
    20641 aaccggcaaa ggccgaaacc agcataatca gcgtcgattc cggcaggtgg aagtttgtca
    20701 ccagcgcatc gaccactttg tactggaagc ccggatagat aaagatttgg gtatcgtcaa
    20761 agaacggttc gataagatcg tttttcgccg cctgagccgc gctttccagc gaacgtactg
    20821 aggtggtgcc aaccgcaatc acccggttac cgcgcgcttt cgccgccagt accgcgtcta
    20881 ccacatcctg cggcacttca gcgtattccg agtgcatgat gtgatcttca atggtgtcga
    20941 cgcgcaccgg ctggaaggtg cccgcaccaa cgtgcaacgt cacaaacgcc atctccacgc
    21001 ctttggcgcg taatttttcc agcaaaggtt cgtcaaaatg cagacctgcg gtcggggctg
    21061 caaccgcgcc cggtttttcg ctataaacgg tttgataaag ttcgcggtca gcgtcttcgt
    21121 ccggacggtc gatatacggc ggcagcggca tatggccgat gctgttgaga atatccagca
    21181 ccgagcgttc atcattaaat tcgacttcaa acagtgcgcc gtggcgcgcg gtcattgttg
    21241 cgttaatact ttcgtcatcg cccagcagca gttctgcgcc aggttttggc gctttcgagg
    21301 cgcgaatatg cgcaagaatg cgtttgtcgt cgagcatccg ttcaaccagc acttcaatct
    21361 tgccgccgct ggctttacgc ccaaacaggc gcgccgggat cacgcgggta ttattaaaaa
    21421 ccagaagatc gccggggttg agcttatcaa gtaaatcggt gaaagtaccg tgcgtcagcg
    21481 cgcccgtcgg cccgtccagc gacagtaaac gacagctact gcgttcaggc atgggatagt
    21541 gggcaatcag ggattcgggc aattcaaagg agaaatcggt aacgcgcatg acactgactc
    21601 agactaaaat aagaggcggg tagtctagtg ccggggcgct ctccctgcaa cctttacccc
    21661 cttttggata gaatataatc gatgaatttt ttagctcacc tgcatttagc ccatctcgcg
    21721 gaaagctcgc tttccggcaa tttactggct gatttcgtac gcggaaatcc tgaagaaagt
    21781 tttccgcccg acgtcgtggc tggcattcat atgcatcgac gtatcgacgt attaaccgac
    21841 aatctgccgg aagtccgcga agcacgggaa tggtttcgta gtgaaactcg ccgcgttgcg
    21901 cctattacgc tggatgtcat gtgggatcac tttctttccc gccactggtc gcagctgtcg
    21961 ccggactttc cgctacagga atttgtctgt tatgcccgcg agcaagtgat gacgattttg
    22021 ccggactcac cgccacgttt tatcaatctg aacaattact tgtggtcaga gcagtggctg
    22081 gtgcgctatc gcgatatgga tttcatccag aacgtgttaa acggcatggc aagccgccgc
    22141 ccacgtctgg atgccctgcg tgactcctgg tacgatttag acgctcatta tgacgccctc
    22201 gaaacccgct tctggcagtt ttatccgcgg atgatggcgc aggcgtcaca caaggcgtta
    22261 taaaacgtta gttcatccat accgtagccg aaatggcagg caaagccaga atcccgtcag
    22321 tcagttgccc atgcctttct ttgcattgcc attgcacggc attgagcaac ggtgacgcgg
    22381 gtagcacgac ttcacaagct tcgccacggt tgattgccac cagtacacgc tgctgattca
    22441 gcacgcggac aaataccacc acgttatctt ccgcatacag cacctgacag ccgccatgac
    22501 gtagcgcctg acttttctta cgcagcgcaa tcattcgctg gtacagcgcg aataacgccg
    22561 tatcctgctt ttccacctgc caggggaacg gtttacggca aaacggatcg tttttgccat
    22621 ccagtcctac ttcatcaccg taataaatgc acggtacacc aggccaggtg aacagccaga
    22681 ccaccgccag cggcaggcgc gcaatatccc gaccgagcag cgttttaaat cgcgcagtat
    22741 cgtggctgtc gagctgatta aacatacgta attgttgttg atgagaaagc cctgcgcggt
    22801 aattatccat ccaggccata caggtttggg catcaatttg ctgcggatcg taagagatat
    22861 cggtattggc aagaaatccc cacaacggga atgtgaagcc acgatagttc atggcggcat
    22921 cttccacatc ggcctgtaac cattgccgtg catcgccaaa atgttcgccg acaatatacg
    22981 cttccggctg ggtttctttc gccgcttcgg tgctcccagc aacgtgctgc atattattgc
    23041 gcgccccacc cgcctcccct agcatatgca ccacatccag ccgccagccg tccatactcc
    23101 acggcgcttt cagccaatgg cggacaatac tgtcttcccc gcgataaatt tcattcacca
    23161 gactttccga ctgataatcc agcttcggca agctggcata gccaagccag tcgagcgccg
    23221 tgccatcatc actaaacgag taccagtcgc gccagggcga ttcagggttg tgacaagctc
    23281 cccccgtgcc acgattgtgc ctatcaaacc aggcatggga atcgccactg tggttaaaca
    23341 cgccatccag ccccagccgc attcccagct gctgcgtatt gtggcgtaag cgcagcaacg
    23401 ccccatcacc gccaaactgc ggatcgacat ggcgataatc ctcggtatcg tatttatgta
    23461 cgctgggagc tttaaacacc ggattgagat acagcgccgt cacgccaagc tttttcagat
    23521 acggcagttt ttcgcttatc ccgtccagat cgccgccata gaacgttgat ccgcccgcct
    23581 gcgccgtgac cggttcatcc cagtcacgca agatgatctc ttgtccggct gcatgatggt
    23641 aatagacatg atcctgttca gcttcacgag gaagactacg cgcaaaacga tcagggaaga
    23701 tctgataaaa aatctgatcc gcagcccatt gtgggccgat atccggtaca tcgacggcaa
    23761 actgctccag tcgtgccggc ggcattcggc tgaagccctg cggtgtaaac caacgctggc
    23821 gatcgtgcca cagcagtttg aaactgtaac gccgccgggg ttgtccgctg gagagatcaa
    23881 tcgccgcacg ccatgcggtg acgccaggct gctgctgact gcgatgctta tgcatcgata
    23941 ctgacatttc ttcgttatcg tgttctgtac gcagcataat gcgctgcggt gggtcttcgc
    24001 ccgtcagcca cagtgtaatg agcagttgat ctttgctttg tttaacaaat gggggcaccg
    24061 gcaggtgcca tgcatttaac atcataattc ccctgtgatg aaaatctgat caccttgcca
    24121 tagcctaatg caggatcact catcatctga aggatatttg ggggaggagg acgggggcgg
    24181 ataactttcg gaatagccgg atcaggattt cctcctgaac cggcttacag aacgcaagga
    24241 ttactggttt tcagccagct gacgatcgtg gcgacgctta aacatccagc caatcaacag
    24301 cacaacaatc cacgcgaaac cgacatacag cgagatacgc gtatccgggt gataaccaat
    24361 caacccgata ataaagagca ggaaaatcag cccgccgatg gtcgttgcta ccccacccgg
    24421 cactttaaat ttcagcgcct taacttcttc tggcggcaaa cagcgacgga aggcaatttg
    24481 cgacagcagg atcataatcc acacccacac cgtggcgaag gttgccagcg aagcgatcac
    24541 caggaagacg ttttccggca tgatgtagtt cagatacacc gcaaacagca gcgcggtagt
    24601 catcaccagc accgtaaccc acggaatacc gcgacgcgac gttttgctga aaattttcgg
    24661 cgcgctgccc tgctctgcca taccgtggag catacggcct acgccaaaga cgtcactgtt
    24721 aattgccgat agcgaagcgg tcagcacaac aaagttaaga atgctagcgg caaaggtaat
    24781 gcccatatgc tggaacgtca gcacgaacgg gctaccggca gtgccaacct gattccacgg
    24841 gtagatagac ataatgacga acagcgtacc gacgtagaac accagaatac gcatcggcac
    24901 ggagttaatc gcacgcggta tcgatttctc aggatctttc gcttcaccgg cggtaatccc
    24961 gataatttcg atcccaccgt aagcaaacat caccatttgc aacgacatca ccatcccaag
    25021 ccagccgtta ctgaagaagc cgccgttgct ccacagatta tgaataccgg tcggttgccc
    25081 gccgttgcca atcccccaga tgatgatgcc gaaaccagcg acaatcatga tgatgatggt
    25141 ggcgacttta aagaacgaga accagaattc cagctccccg aagaccttca cgctcatcag
    25201 gtttacggcg caaatgatca gcaccacgct cagtacccaa atccagtgcg gcaccgtcgg
    25261 gaaccagaca cccatataga taccaaaagc ggtcacatcg gcgatggcga caataaggat
    25321 ttcaaagcag taggtccagc cggtaatgta acctgccagc gggccgaggt tttcctgcgc
    25381 ataacgcgag aaagagctgg cggccgggtt atgtaccgac atttccccca gcgcacgcat
    25441 aatgatatac gccgcgatac caccgataat ataggccaac aacacgctcg gaccggccat
    25501 tttgatggcg tctgccgaac cgtaaaacag cccggtacca attgctgaac ccagtgccat
    25561 aaagcgtatg tggcgggtac ttagcccacg ctttagcttg ttcttacttt ccatcaattc
    25621 ctaacccatc aacacaataa aaaaccacgg ggcatcgcag ccccgtggtt aaaacaaatg
    25681 ttcagtgatt tagtgagcgc tggaggtcac ctgacgacct gccgcgcgat cccagataat
    25741 ggccagaacc accatcacca ctgttggcat taaccacgcc agaccttgtt cggccagcgg
    25801 taaacgctgc gcccaggacg gtaagatatc gctgaatgca gatgctttga tcccgtcgag
    25861 aataccaaaa agcaggctga taaacatcgg cggagcaatc acgcgggacg aattatgcca
    25921 ccatgagcgt gtaaaactta atacaaccag tgcgatacac ggcggataaa tagcggtcag
    25981 caccggtacg gagatctgga tcagctggct taagccgagg ttagaaacca ccatcgagaa
    26041 gccgccgagg ataaacacca gcgtacgata agagagcggt acgtactggg caaagaattc
    26101 tgcacaagca caggtcaggc caactgccgt taccaagcag gcgatgaaga ttaacgccgc
    26161 cagcaggaag ctaccgccgc cgccaaaggt gtgctgaacg taagcatgca gaatagcagc
    26221 gccgtttgca gactgatcga ccagcgacgc gctgtctgaa cccagacgga acagcgccag
    26281 gtacagcaga gtcagaccaa cacccgccat caggccagcc cagacggtat aacgggtcag
    26341 cagacgcgct tcggtaacgc cacgagaacg cgccgcgtta acaataacga taccaaacac
    26401 cattgcgccc agcgtatcca tggtcagata gccgttaacg aagccgttag aaaacgcagc
    26461 gttttgataa gcctcagtcg ccgtgctgat agaacccgcc ggccagacaa tcgcggcaac
    26521 agacaggatg accagcgcga taattttcag cggcgcaagg aagttgccca cggtatccag
    26581 cagcttgccc ggatagagcg aaaccagaat aacgatagcg aaatagacca ggctgtagat
    26641 aaacagcggc agcgcggaat caccagtcag cggcgcaatc cccacttcaa aggaaacggt
    26701 agctgtacgc ggcgtagcga aaagcggccc caccgccagg taacaaaccg ttgccagcag
    26761 cacgccagcg actttaccga ttggcgtgct gaggctgtca acaccgccgc caacttttgc
    26821 cagcgccact accgttaata ccggcaggcc aacggcagta atgaggaagc cgaatgccgc
    26881 agtccagacg tgttcgccag cctgcaagcc gaccattgga gggaaaataa tgttacctgc
    26941 gccgacgaac aacgcaaatg tcataaagcc cagagcgatg atatcgcgcg atcttaattg
    27001 atgggtcata aatcatactg cctgtggatg tggtgttgta acgttaaatt tttgtgccaa
    27061 tccctgggga tgatacagcg gtatatttaa acaattttag cgggaaatac tcccaaccag
    27121 gcgcggcgaa gaatagtttt tcgatttacc gcgttaatgc agtcagtatg acagcacctg
    27181 aagcgcaatt taaacgctta taacgtttta aagcaaggag ggatctaaaa accagaggta
    27241 tatagcgata agaaatcacc aaccagtata aaacactatt tccgtgaaaa agacatggct
    27301 aatcatgcga acaaaaaatc accaaccgtt gtaaaatcta acgcccgtat tttgactgca
    27361 aagaaaataa gccagcatcg actggcttat ggcaataaaa gatgacaaag gcggattaat
    27421 cgctgttttt ggcaattaaa cgttccggga taacaaaact gaaacgtgtt ccttttccta
    27481 ctgtactctc aatattcagg cgactttcgt gatgattcac agcatgtttc acgatcgcta
    27541 accctaatcc gctaccgccg gtttgccggg aacgcgcttt atcaacgcga taaaaacgct
    27601 cggtcaggcg cggaatatgc tccggtgcaa tgcccggtcc gttatcttca acgctaaatt
    27661 cggcaccgtg cggcactcgc tgccagcgta cggtgatatg cgtgccttct ggagtatgat
    27721 tcacggcgtt atagaccagg ttcgaaatcg cactgcgtag ctgatcttcg ttgccagaca
    27781 ctttgaggcc gttatctatc tcaaaggtaa atgtctgttt tttctgactc agagtctgag
    27841 cctcgcgctc aacaacgcgc agcatcatcg gcacatcaac cttttcattg agcaattgcg
    27901 tcggcgcggc ttcaattttc gacagcgtta gcaactgctt caccagccct tccatccgct
    27961 gggtctgctc gcgcatggtg tgcaacgctt tttcgcgtac cgcgccttcc agcggctgct
    28021 catccatcat ctccaggtaa ccctgtaaca cggtcaatgg cgtacgtaac tcatggctca
    28081 cgttggcaaa aaagttacgc cgcgcccctt ccagttgatg catttgcgtg acatcacgcg
    28141 ccaccatcag caactgtttg tgggtataag gcatgacgcg aatttccaga tgccgcccgg
    28201 tgttgagcac cagattgagc gggcgagaaa aatcacgcgt tttcagatat tgcgtaaact
    28261 ccgggtaacg cagtaggtta aggatgttct gcccgttatc ttccggccag cgcaaaccaa
    28321 gaatttgttg cgccagaccg ttacaccaga aaataccgct ctcttccgtg gtcagcacca
    28381 ccgcgtcggg cagcgactcc gcgccgctac gaaagcgttt aatcagattg cccagttcac
    28441 ggcggcgttt tttatttcgc agctgcatct ggtgtaagcc gtatagtagc ggttcccagc
    28501 taccacgccc cggtggcggg gtcatactgc gatccaccca cagccaccat gaaaggcgca
    28561 ataaattcca gaaatgccag ataagcagtc ctgttaccga tgccagcaaa aaccagggca
    28621 ggtaaccaaa aaatgcaccc aggatgaaag ccgggaggca gcaaagtagc agctccagca
    28681 ccagcctttt ccacgacagc cgttccagca cgtaagatac tcccgttaag aaatcataag
    28741 ccctgctctg cgtccgatga gcaaggcgtt aaaagcgggt tgaaaaacga tatcctgtac
    28801 cgcgcacggt ctgcaccatg cggtcatgcc cgccgggctc cagtgcttta cgcaggcgac
    28861 gaatgtggac atcgaccgtg cggtcttcca catacacgtt agttccccag acgtggttta
    28921 acagctgctc gcggctgtac acgcgctcag gatgcgtcat aaaaaagtgc agcagtttaa
    28981 attctgtcgg ccccatctcc agcggctctt cgcccgccat cactcggtga gatgtcgggt
    29041 cgagacttaa tccctgcatc tcaatcacct cttccaccgc cattggcgaa atacggcgca
    29101 ttaccgcttt gattcgcgcc accagctcct tcggcgaaaa cggcttggtg atatagtcat
    29161 ccgcgccggt ttcaaggccg cgcacgcgat cttcttcttc ccctctggcg gtcaacatca
    29221 ccactggaat atcccgggtc atcgactcgc gcttaaggtg tttgatgaac tggataccgg
    29281 agccgccagg taacatccag tcaaggagaa ttaaatccgg ccagggttca ttcagttgat
    29341 tcacagcaca gtcataatct tccgcttcga ccggctgaaa gccattttgt tcgagcacga
    29401 agcagaccat ttcacgaatt ggagcttcat cttctacgac cagaatacgt ctcgccatga
    29461 tttgccctgt tgtaataaat aggttgcgat cattaatgcg acgtcattat gcgtcagatt
    29521 tatgacagat ttatgaaaag ctcgtcgcac atatcttcag gttattgatt tccgtggcgc
    29581 agaaaaaagc aaatggcaca tctgtttggg tataatcgcg cccatgcttt ttcgccaggg
    29641 aaccgttatg cgcatccttc acacctcaga ctggcatctc ggccagaact tctacagtaa
    29701 aagccgcgaa gctgaacatc aggcttttct tgactggctg ctggagacaa cacaaaccca
    29761 tcaggtggat gcgattattg ttgccggtga tgttttcgat accggctcgc cgcccagtta
    29821 cgcccgcacg ttatacaacc gttttgttgt caatttacag caaactggct gtcatctggt
    29881 ggtactggca ggaaaccatg actcggtcgc cacgctgaat gaatcgcgcg atatcatggc
    29941 gttcctcaat actaccgtgg tcgccagcgc cggacatgcg ccgcaaatct tgcctcgtcg
    30001 cgacgggacg ccaggcgcag tgctgtgccc cattccgttt ttacgtccgc gtgacattat
    30061 taccagccag gcggggctta acggtattga aaaacagcag catttactgg cagcgattac
    30121 cgattattac caacaacact atgccgatgc ctgcaaactg cgcggcgatc agcctctgcc
    30181 catcatcgcc acgggacatt taacgaccgt gggggccagt aaaagtgacg ctgtgcgtga
    30241 catttatatt ggcacgctgg acgcgtttcc ggcacaaaac tttccaccag ccgactacat
    30301 cgcgctcggg catattcacc gcgcacagat tattggcggc atggaacatg ttcgctattg
    30361 cggctccccc attccactga gttttgatga atgcggtaag agtaaatatg tccatctggt
    30421 gacattttca aacggcaaat tagagagcgt ggaaaacctg aacgtaccgg taacgcaacc
    30481 catggcagtg ctgaaaggcg atctggcgtc gattaccgca cagctggaac agtggcgcga
    30541 tgtatcgcag gagccaccag tctggctgga tatcgaaatc actactgatg agtatctgca
    30601 tgatattcag cgcaaaatcc aggcattaac cgaatcattg cctgtcgaag tattgctggt
    30661 acgtcggagt cgtgaacagc gcgagcgtgt gttagccagc caacagcgtg aaaccctcag
    30721 cgaactcagc gtcgaagagg tgttcaatcg ccgtctggca ctggaagaac tggatgaatc
    30781 gcagcagcaa cgtctgcagc atcttttcac cacgacgttg cataccctcg ccggagaaca
    30841 cgaagcatga aaattctcag cctgcgcctg aaaaacctga actcattaaa aggcgaatgg
    30901 aagattgatt tcacccgcga gccgttcgcc agcaacgggc tgtttgctat taccggccca
    30961 acaggtgcgg ggaaaaccac cctgctggac gccatttgtc tggcgctgta tcacgaaact
    31021 ccgcgtctct ctaacgtttc acaatcgcaa aatgatctca tgacccgcga taccgccgaa
    31081 tgtctggcgg aggtggagtt tgaagtgaaa ggtgaagcgt accgtgcatt ctggagccag
    31141 aatcgggcgc gtaaccaacc cgacggtaat ttgcaggtgc cacgcgtaga gctggcgcgc
    31201 tgcgccgacg gcaaaattct cgccgacaaa gtgaaagata agctggaact gacagcgacg
    31261 ttaaccgggc tggattacgg gcgcttcact cgttcgatgc tgctttcgca ggggcaattt
    31321 gctgccttcc tgaatgccaa acccaaagaa cgcgcggaat tgctcgagga gttaaccggc
    31381 actgaaatct acgggcaaat ctcggcgatg gtttttgagc agcacaaatc ggcccgcaca
    31441 gagctggaga agctgcaagc gcaggccagc ggcgtcacgt tgctcacgcc ggaacaagtg
    31501 caatcgctga cagcgagttt gcaggtactt actgacgaag aaaaacagtt aattaccgcg
    31561 cagcagcaag aacaacaatc gctaaactgg ttaacgcgtc aggacgaatt gcagcaagaa
    31621 gccagccgcc gtcagcaggc cttgcaacag gcgttagccg aagaagaaaa agcgcaacct
    31681 caactggcgg cgcttagtct ggcacaaccg gcacgaaatc ttcgtccaca ctgggaacgc
    31741 atcgcagaac acagcgcggc gctggcgcat attcgccagc agattgaaga agtaaatact
    31801 cgcttacaga gcacaatggc gcttcgcgcg agcattcgcc accacgcggc gaagcagtca
    31861 gcagaattac agcagcagca acaaagcctg aatacctggt tacaggaaca cgaccgcttc
    31921 cgtcagtgga acaacgaact ggcgggttgg cgtgcgcagt tctcccaaca aaccagcgat
    31981 cgcgagcatc tgcggcaatg gcagcaacag ttaacccatg ctgagcaaaa acttaatgcg
    32041 cttgcggcga tcacgttgac gttaaccgcc gatgaagttg ctagcgccct ggcgcaacat
    32101 tccgagcaac gcccactgcg tcagcgcctg gtcgcgctgc atgggcagat tgttccccaa
    32161 caaaaacgtc tggcgcagtt aatggtcact atccagaatg tcactctgga acagacgcaa
    32221 cgtaatgtcg cacttaacga aatgcgccag cgttataaag aaaagacgca gcaacttgcc
    32281 gatgtgaaaa ccatttgcga gcaggaagcg cgcatcaaaa cgctggaagc tcaacgtgca
    32341 cagttacagg cgggtcaacc ttgtccactt tgtggttcca ccagccaccc ggcggtcgag
    32401 gcgtatcagg cgctggagcc tggtgttaat cagtctcgat tactggcgct ggaaaacgaa
    32461 gttaaaaagc tcggcgaaga aggtgcggcg ctgcgcgggc aactggatgc attaacgaag
    32521 cagcttcagc gcgatgaaaa cgaagcgcaa agcctccgac aagatgagca agcacttact
    32581 caacaatggc aagccgtcac gaccagcctc aatatcacct tgcagccaca ggacgatatt
    32641 caaccgtggc tggatgcaca agatgagcac gaacgccagc tgcggttact cagccaacga
    32701 catgaattac aagggcagat tgccgcgcat aatcagcaaa ttatccagta tcaacagcaa
    32761 attgaacaac gccagcagca gctcttaacg gcattgacgg gttatgcact gacattgcca
    32821 caggaagatg aagaagagag ctggttggcg acacgtcagc aagaagcgca gagctggcag
    32881 caacgccaga acgaattaac cgcgctgcaa aaccgtattc agcagctgac gccgattctg
    32941 gaaacgttgc cgcaaagtga tgatctcccg cacagcgaag aaactgtggc gctggataac
    33001 tggcggcagg ttcatgaaca atgtctcgca ttacacagcc agcagcagac gttacagcaa
    33061 caggatgttc tggcggcgca aagtctgcaa aaagcccagg cgcagtttga caccgcgcta
    33121 caggccagcg tctttgacga tcagcaggcg ttccttgcgg cgctaatgga tgaacaaaca
    33181 ctaacgcagc tggaacagct caagcagaat ctggaaaacc agcgccgtca ggcgcaaacg
    33241 ctggtcacac agacagcaga aacgctggca cagcatcaac aacaccgacc tgacgggttg
    33301 gctctcactg tgacggtgga gcagattcag caagagttag cgcaaactca ccaaaagttg
    33361 cgtgaaaaca ccacgagtca aggcgagatt cgccagcagc tgaagcagga tgcagataac
    33421 cgtcagcaac aacaaacctt actgcagcaa attgctcaaa tgacgcagca ggttgaggac
    33481 tggggatatc tgaattcgct aataggttcc aaagagggcg ataaattccg caagtttgcc
    33541 caggggctga cgctggataa tttagtccat ctcgctaatc agcaacttac ccggctgcac
    33601 gggcgctatc tgttacagcg caaagccagc gaggcgctgg aagtcgaggt tgttgatacc
    33661 tggcaggcag atgcagtacg cgatacccgt accctttccg gcggcgaaag tttcctcgtt
    33721 agtctggcgc tggcgctggc gctttcggat ctggtcagcc ataaaacacg tattgactcg
    33781 ctgttccttg atgaaggttt tggcacgctg gatagcgaaa cgctggatac cgcccttgat
    33841 gcgctggatg ccctgaacgc cagtggcaaa accatcggtg tgattagcca cgttgaagcg
    33901 atgaaagagc gtattcctgt gcagatcaaa gtgaagaaga tcaacggcct gggctacagc
    33961 aaactggaaa gtacgtttgc agtgaaataa ctattcagca ggataatgaa tacagagggg
    34021 cgaattatct cttggccttg ctggtcgtta tcctgcaagc tatcacttta ttggctacgg
    34081 tgattggtag ccgttctggt ggttgtgatg gtggtatgaa aaaagtcatt ttatctttgg
    34141 ctctgggcac gtttggtttg gggatggccg aatttggcat tatgggcgtg ctcacggagc
    34201 tggcgcataa cgtaggaatt tcgattcctg ctgccgggca tatgatctcg tattatgcac
    34261 tgggggtggt ggtcggtgcg ccaatcatcg cactcttttc cagccgctac tcactcaaac
    34321 atatcttgtt gtttctggtg gcgttgtgcg tcattggcaa cgccatgttc acgctctctt
    34381 cgtcttacct gatgcttgcc attggtcgcc tggtttccgg ctttccgcat ggcgcatttt
    34441 ttggcgtcgg cgcgatcgtg ttatcaaaaa ttatcaaacc cggaaaagtc accgccgccg
    34501 tggcggggat ggtttccggg atgacagtcg ccaatttgct gggcattccg ctgggaacgt
    34561 atttaagtca ggaatttagc tggcgttaca cctttttatt gatcgctgtt tttaatattg
    34621 cggtgatggc atcggtctat ttttgggtgc cagatattcg cgacgaggcg aaaggaaatc
    34681 tgcgcgaaca atttcacttt ttgcgcagcc cggccccgtg gttaattttc gccgccacga
    34741 tgtttggcaa cgcaggtgtc tttgcctggt tcagctacgt aaagccatac atgatgttta
    34801 tttccgtttt ttcggaaacg gcgatgacct ttattatgat gttagttggg ctagggatgg
    34861 tgctgggaaa tatgctaagt ggcaggattt caggacgtta ttcaccactg cgcattgcag
    34921 cagtgactga ctttatcatt gtactggcac tgctgatgct ctttttctgc ggcggcatga
    34981 aaacaacgtc gcttattttt gcttttattt gttgcgcggg attatttgcc ctttcagcac
    35041 ctctgcaaat attgttactg caaaacgcca aaggcggaga gttattaggt gccgcaggtg
    35101 ggcaaatagc gtttaacctc ggtagcgccg tcggcgcata ttgcggaggt atgatgctga
    35161 cgctggggct ggcatataat tacgtggcgc tgcctgccgc cctgctttcg tttgctgcga
    35221 tgtcatcgtt gctgctgtat ggtcgctata agcgccagca agcggcggat agtccggtgc
    35281 tggcgaaacc actggggtag attactgtca cggtgcgcgt tgcttattgc cggatgcggc
    35341 gtgaacgcct tatccgccct acgcggttct ggcacatttt gtaggcctga taagacgcgg
    35401 caagcgtcgc atcaggcatg atgcgccaat tgcctacgtt ttttactctt gtggccataa
    35461 ccacgcagcg ccgcgtacgc cgctggaatc accgtgcttc gccttacgca ccggcgtttc
    35521 acattcgccg ccgaagacaa attgtttaat caactgccca accgtttgat ataaacggtc
    35581 tacattgctc atcccgcccc ccaggacaat cacatccgga tcgagaatat tcacgacatg
    35641 tgccagcgat tttgccagcc gcagctcgta gcgacgcaat gccagttccg ctaccggatc
    35701 gctttcttca accaggcgga taatttcact gcctttcagc gcatgtccgc tcaaacgacg
    35761 ataatccgtc gcgaatcccg tgcccgaaat aaaggtttca atacaacctt gtttaccgca
    35821 ataacaaggg acttcctcgc gataacgcag ctcgtcttca tccatccacg gtagcggatt
    35881 gtgtccccac tcgcctgccg tgccattgcc gccgatatgc gcccgcccat ttaatgccac
    35941 gcccgcgccg catcccgtgc cgataatcac ggcaaatacc gtctgcgctc ccgctgccgc
    36001 gccatctact gcttctgaaa ccgccagaca gttggcatca tttgccagcc gcacttcccg
    36061 ctgcaacctc gcgcttaagt ctttatcgaa tggctgaccg ttgagccagg ttgaattggc
    36121 attcttcacc acaccggtgt agggcgaaat cgaacctgga atgcccatac ctaccgttcc
    36181 gcgctgccct gtcgcctgct ccgccatatc aaccaacgtg gcgatcgttt caatagtctg
    36241 ccggtaatca tcacgcggcg tgggcagacg atggcggtac aactgctccc ctgcatcgcc
    36301 cagtgcaatc acttcagttt tggtgccgcc taaatcgata cctatacgca ctgtactctc
    36361 cttatttttt tcaatatcaa tagcgtagag acggacaacc ggattggcaa tgcaaggccg
    36421 ccgacaattc gttatcatgc ccgctaaatt taacgacaag gccgtggaaa ttatcatgct
    36481 gtggttcaaa aatttaatgg tttaccgtct tagccgcgag atttcgctgc gtgcagaaga
    36541 gatggaaaaa cagctagcct cgatggcatt taccccatgc ggcagccagg acatggcgaa
    36601 gatgggctgg gttcctccga tgggatcgca cagcgatgcg ttaacgcacg ttgccaatgg
    36661 tcaaattgtt atctgcgcgc gcaaagaaga aaaaatcctc ccgtctccgg tgattaaaca
    36721 ggcgctggaa gcgaaaatcg ccaaactgga agcggaacag gcgcgtaagc tgaagaaaac
    36781 cgaaaaagat tcgctgaaag acgaagtgct gcactctctg ctgccgcgtg ctttcagccg
    36841 ttttagccag acaatgatgt ggatcgacac ggttaacggt ttgattatgg tggactgcgc
    36901 cagtgccaaa aaagcggaag atacgctggc attactgcgt aaaagcctgg ggtcgttacc
    36961 ggttgtacca ttgagcatgg aaaacccgat tgaactgacg ctgaccgaat gggttcgctc
    37021 cggtagtgcg gcacagggct tccagctgct tgatgaggcc gagctgaaat cgttgctgga
    37081 agatggcggc gtgatccgcg cgaagaaaca agatctgacc agcgaagaga tcaccaatca
    37141 cattgaagcc ggaaaagtgg tgactaaact ggcgctcgac tggcagcagc gcattcagtt
    37201 tgtgatgtgc gacgatggtt cgctcaagcg tcttaagttc tgcgacgagc tgcgcgatca
    37261 aaacgaagat atcgaccgtg aagatttcgc cctgcgtttt gatgccgatt tcatcctgat
    37321 gactggtgaa ctggcagcgt taattcaaaa cctgattgaa ggattaggtg gcgaagcaca
    37381 acgttaattg ctgattttcc tttaatgccg gatgcgacgc ctgccgcgtc ttatccggcg
    37441 tacgaagcca caccaggcat ataattattc gctacggcga gcaataattt ttagcgcagc
    37501 aatattatgc gttttacgcc gtaactgatc taatcgcctc atccctattc gcctgtcagc
    37561 agcacgcaga atattcacta acccccattt atcgctggta aattgttttg ttaacagctc
    37621 cgcttttggg gtatcaatat attcacgcaa taaatcagaa attgaacaaa tatccgtcag
    37681 gtaaggatac cagcaagttt caccgtgaaa attacgcact ccagcgttac ctgcaataaa
    37741 gtctttcggg taatcccaga tcacgttttt atccaacgtg atccaataac gcggtaaatc
    37801 ggtacttcca ccattttggc tgcgcatgag ataacgggta cagtgaattt gcaggttgat
    37861 cgttggcgtc agtaagtcat agatctcccg ctgtaacttg ctccatggat gttgtgtcat
    37921 tgtttttcct caagccggtg acgcgcttca ctgcgaattt tccgcgttgc cccggttttc
    37981 actaaatcac gtaactgcga aagatcgggt tgttcgacaa ataagagtaa tgatagatcc
    38041 tcttttaacc acgccgtttt cacatcggca gccagctgtg gattattaat agccagcgac
    38101 cgatcctgag actcagtcaa tgtcgttaac aacgacgccg gggtatgtgc gttgcgtaat
    38161 gccgtttggc gcaaatgatc attgcgactt tgcagcaatg cacgccacca ttcatcatct
    38221 gatttatctt ttatcgtagc cagttccgca aaaaatacct ggagatcttc actgatattt
    38281 atatcaacct tgatgaatta tgaatatatc ggaaattgaa aaaatcgcag caaagga
//
LOCUS       DADMKF010000022        37809 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.022, whole genome shotgun sequence.
ACCESSION   DADMKF010000022 DADMKF010000000
VERSION     DADMKF010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 37809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37809
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.022"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(268..885)
                     /gene="tdk"
                     /locus_tag="JNC24_001024"
     CDS             complement(268..885)
                     /gene="tdk"
                     /locus_tag="JNC24_001024"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HAY9157838.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            1490..1903
                     /gene="hns"
                     /locus_tag="JNC24_001025"
     CDS             1490..1903
                     /gene="hns"
                     /locus_tag="JNC24_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator H-NS"
                     /protein_id="HAY9157839.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(2047..2955)
                     /gene="galU"
                     /locus_tag="JNC24_001026"
     CDS             complement(2047..2955)
                     /gene="galU"
                     /locus_tag="JNC24_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAY9157840.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(3157..4170)
                     /gene="rssB"
                     /locus_tag="JNC24_001027"
     CDS             complement(3157..4170)
                     /gene="rssB"
                     /locus_tag="JNC24_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HAY9157841.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(4262..5167)
                     /gene="rssA"
                     /locus_tag="JNC24_001028"
     CDS             complement(4262..5167)
                     /gene="rssA"
                     /locus_tag="JNC24_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HAY9157842.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVERKMDELLPLVRTNI"
     gene            5280..5738
                     /locus_tag="JNC24_001029"
     CDS             5280..5738
                     /locus_tag="JNC24_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HAY9157843.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELMAGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            5788..6630
                     /gene="purU"
                     /locus_tag="JNC24_001030"
     CDS             5788..6630
                     /gene="purU"
                     /locus_tag="JNC24_001030"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HAY9157844.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMAEAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            6790..6874
                     /locus_tag="JNC24_001031"
     tRNA            6790..6874
                     /locus_tag="JNC24_001031"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6824..6826,aa:Tyr,seq:gta)
     gene            6922..7053
                     /locus_tag="JNC24_001032"
     ncRNA           6922..7053
                     /ncRNA_class="other"
                     /locus_tag="JNC24_001032"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(7237..7914)
                     /gene="narI"
                     /locus_tag="JNC24_001033"
     CDS             complement(7237..7914)
                     /gene="narI"
                     /locus_tag="JNC24_001033"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HAY9157845.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRLFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(7914..8624)
                     /gene="narJ"
                     /locus_tag="JNC24_001034"
     CDS             complement(7914..8624)
                     /gene="narJ"
                     /locus_tag="JNC24_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HAY9157846.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQAQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVMFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(8621..10159)
                     /gene="narH"
                     /locus_tag="JNC24_001035"
     CDS             complement(8621..10159)
                     /gene="narH"
                     /locus_tag="JNC24_001035"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HAY9157847.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLEVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(10156..13899)
                     /locus_tag="JNC24_001036"
     CDS             complement(10156..13899)
                     /locus_tag="JNC24_001036"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HAY9157848.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVSTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFLDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
     gene            complement(14292..15683)
                     /gene="narK"
                     /locus_tag="JNC24_001037"
     CDS             complement(14292..15683)
                     /gene="narK"
                     /locus_tag="JNC24_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HAY9157849.1"
                     /translation="MSHSSAPERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATDTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
     gene            16022..17818
                     /gene="narX"
                     /locus_tag="JNC24_001038"
     CDS             16022..17818
                     /gene="narX"
                     /locus_tag="JNC24_001038"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HAY9157850.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDEQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLLDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            17811..18461
                     /gene="narL"
                     /locus_tag="JNC24_001039"
     CDS             17811..18461
                     /gene="narL"
                     /locus_tag="JNC24_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HAY9157851.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(18462..19856)
                     /gene="ychO"
                     /locus_tag="JNC24_001040"
     CDS             complement(18462..19856)
                     /gene="ychO"
                     /locus_tag="JNC24_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HAY9157852.1"
                     /translation="MSRFVPRIIPFYLLLLAAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDDGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLLDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVRVEQYFGERVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            20041..20394
                     /gene="ychN"
                     /locus_tag="JNC24_001041"
     CDS             20041..20394
                     /gene="ychN"
                     /locus_tag="JNC24_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HAY9157853.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            complement(20438..21133)
                     /locus_tag="JNC24_001042"
     CDS             complement(20438..21133)
                     /locus_tag="JNC24_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HAY9157854.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPNE
                     GPVWIFGYGSLMWNPALEFTELCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(21291..21521)
                     /gene="chaB"
                     /locus_tag="JNC24_001043"
     CDS             complement(21291..21521)
                     /gene="chaB"
                     /locus_tag="JNC24_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HAY9157855.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            21791..22891
                     /gene="chaA"
                     /locus_tag="JNC24_001044"
     CDS             21791..22891
                     /gene="chaA"
                     /locus_tag="JNC24_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HAY9157856.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLALVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQTKTHQSLFVYEHEDDSDDDDPHHGKPSAH
                     SSVWHAIWLIIHLIAVIAVTKMNASPLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            23294..23401
                     /locus_tag="JNC24_001045"
     CDS             23294..23401
                     /locus_tag="JNC24_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small toxic polypeptide LdrA/LdrC"
                     /protein_id="HAY9157857.1"
                     /translation="MTLAQFAMTFWHDLAAPILAGIITAAIVSWWRNRK"
     gene            23830..23937
                     /locus_tag="JNC24_001046"
     CDS             23830..23937
                     /locus_tag="JNC24_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000141634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small toxic polypeptide LdrA/LdrC"
                     /protein_id="HAY9157858.1"
                     /translation="MTLAKFAMIFWHDLAAPILAGIITAAIVGWWRNRK"
     gene            24365..24472
                     /locus_tag="JNC24_001047"
     CDS             24365..24472
                     /locus_tag="JNC24_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small toxic polypeptide LdrA/LdrC"
                     /protein_id="HAY9157859.1"
                     /translation="MTLAQFAMIFWHDLAAPILAGIITAAIVGWWRNRK"
     gene            complement(24621..25475)
                     /gene="kdsA"
                     /locus_tag="JNC24_001048"
     CDS             complement(24621..25475)
                     /gene="kdsA"
                     /locus_tag="JNC24_001048"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HAY9157860.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(25511..26319)
                     /locus_tag="JNC24_001049"
                     /pseudo
     CDS             complement(25511..26319)
                     /locus_tag="JNC24_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing protein"
     gene            complement(26323..26715)
                     /locus_tag="JNC24_001050"
     CDS             complement(26323..26715)
                     /locus_tag="JNC24_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB family protein"
                     /protein_id="HAY9157861.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            complement(26712..27545)
                     /gene="prmC"
                     /locus_tag="JNC24_001051"
     CDS             complement(26712..27545)
                     /gene="prmC"
                     /locus_tag="JNC24_001051"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HAY9157862.1"
                     /translation="MEYQYWLREAISQLQASESPRRDAEILLEYVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            complement(27545..28627)
                     /gene="prfA"
                     /locus_tag="JNC24_001052"
     CDS             complement(27545..28627)
                     /gene="prfA"
                     /locus_tag="JNC24_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HAY9157863.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            complement(28669..29925)
                     /gene="hemA"
                     /locus_tag="JNC24_001053"
     CDS             complement(28669..29925)
                     /gene="hemA"
                     /locus_tag="JNC24_001053"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HAY9157864.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            30139..30762
                     /gene="lolB"
                     /locus_tag="JNC24_001054"
     CDS             30139..30762
                     /gene="lolB"
                     /locus_tag="JNC24_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein localization protein LolB"
                     /protein_id="HAY9157865.1"
                     /translation="MPQPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            30762..31613
                     /gene="ispE"
                     /locus_tag="JNC24_001055"
     CDS             30762..31613
                     /gene="ispE"
                     /locus_tag="JNC24_001055"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HAY9157866.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            31764..32711
                     /locus_tag="JNC24_001056"
     CDS             31764..32711
                     /locus_tag="JNC24_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HAY9157867.1"
                     /translation="MPDMKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            32835..34514
                     /gene="dauA"
                     /locus_tag="JNC24_001057"
     CDS             32835..34514
                     /gene="dauA"
                     /locus_tag="JNC24_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HAY9157868.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(34569..34847)
                     /gene="ychH"
                     /locus_tag="JNC24_001058"
     CDS             complement(34569..34847)
                     /gene="ychH"
                     /locus_tag="JNC24_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HAY9157869.1"
                     /translation="MKRKNASLLGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            35124..35708
                     /gene="pth"
                     /locus_tag="JNC24_001059"
     CDS             35124..35708
                     /gene="pth"
                     /locus_tag="JNC24_001059"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HAY9157870.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            35825..36916
                     /gene="ychF"
                     /locus_tag="JNC24_001060"
     CDS             35825..36916
                     /gene="ychF"
                     /locus_tag="JNC24_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HAY9157871.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRVGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
BASE COUNT         9410 a         9789 c         9242 g         9368 t
ORIGIN      
        1 tctggggccg tttttttgtt tacccaaagc aacttttcca taaaccgaca gcattagcct
       61 tcatcatatt tgcgacgatg tataacgcct aaacacaggg atattgtact ttacaggtca
      121 caagtcaacg tcggtgctta agagccctgt gaggcgtata gcggcgttaa aaaactgccg
      181 agaagggtat atagcccgga agaagtgcgt aaaacgaact gacaggataa aagtgccctg
      241 ctcaccctgt cagtaaagaa attcttatta atcgtggcga tgcctttcct gaatagccgt
      301 taatgagccg acttgtaacg cctctttata gtgtttacgg catacagaaa cgtatcgttc
      361 attaccacca attaccacct gctcaccttc gttataaggt ctgcctgctt gatcaagacg
      421 cagcaccatg cttgctttac ggccacaaaa acagatggtt tttaattcaa ccagtttgtc
      481 ggaccatgcc agtaagtatt ggctgccaat aaataattca cctcgaaaat cggtacgtaa
      541 accataacaa agtacgggta tatcgagttg atcgacaacc tccgataatt catatacttg
      601 ttgtctggtt aaaaactggc attcatcaac cagtacgcaa tgaattgcct gctgttcatg
      661 ttccgcacga atctcatcaa ataatgatga attttggtta aataattttg caggcgatga
      721 caaacctata cgcgaactga ctttcccggc accaaagcga tcatcaattt ctgccgtata
      781 tacgacagtg cgcatgccgc gttcctggta attgtatgaa gattgcaaca atgctgtaga
      841 cttacccgca ttcattgcgg aatagtagaa atatagctgt gccatcagcc acaggccctc
      901 aatgatatga aataaagttg gctggagttt atcataattc gtcagttttt cagctcaaca
      961 gtatgcacag aagtgggtat atgcgttctc ccttacgaag ccttgcataa tccttctgag
     1021 ctatcattac aactgcctcg cttgttataa gcgggtaaat gactactggt aactattcac
     1081 aatctttaac ctgttgcgca agtaatagcc ctctgttgac ctccaggaga tagtgcaata
     1141 ctaagtccat gctcttattg cgactgttct acttttcatc attcgcttaa tagggaattc
     1201 tcgtaaacac aactaataca gaagactgaa aggtcgtcag cctacgataa tctccccata
     1261 aaatgtgaca tgaatcagga agttttaacc tcacgtgctg cgaaatcatc ggtgtaaata
     1321 gggctatatg ccgcgtcttt tctggctaat tttatgaaaa gatatttatt ggcggcacaa
     1381 aataaagaac aattttgaat tccttacatt cctggctatt gcacaactga atttaaggct
     1441 ctattattac ctcaacaaac caccccaata taagtttgag attactacaa tgagcgaagc
     1501 acttaaaatt ctgaacaaca tccgtactct tcgtgcgcag gcaagagaat gtacacttga
     1561 aacgctggaa gaaatgctgg aaaaattaga agttgttgtt aacgaacgtc gcgaagaaga
     1621 aagcgcggct gctgctgaag ttgaagagcg cactcgtaag ctgcagcaat atcgcgaaat
     1681 gctgatcgct gacggtattg acccgaacga actgctgaat agccttgctg ccgttaaatc
     1741 tggcaccaaa gctaaacgtg ctcagcgtcc ggcaaaatat agctacgttg acgaaaacgg
     1801 cgaaactaaa acctggactg gccaaggccg tactccagct gtaatcaaaa aagcaatgga
     1861 tgagcaaggt aaatccctcg acgatttcct gatcaagcaa taatcttttg tagattgcac
     1921 ttgcttaaaa tcccgccagc ggcgggattt tttattgtcc ggtttaagac aatttaataa
     1981 gaataagcta taaaaaaacg gcgtcgattg ctcaacgccg tttcgtggat aacaccgata
     2041 cggatgttac ttcttaatgc ccatctcttc ttcaagccag gctttaaatt ccgtgccaag
     2101 ggtgttatga cgaataccgt attcaacgaa ggcctgcatg taacctaatt tattaccgca
     2161 gtcatggctc ttccctttca tatgataggc ttccaccgtt tctttttcga tcagcatatc
     2221 aattgcgtcg gtgagctgaa tttcatcacc agctcccgga ggggtttttg ccagcaacgg
     2281 ccaaatatcc gcgctaagta cgtaacgacc cacaatagcg agattagacg gcgcaacatc
     2341 cgctttcggt ttttctacca caccaaccat cggtacgctt tcacccggcg ctaattcaac
     2401 gcctttgcaa tccacaacgc catatgcggt cacatcagca accggttcaa ccatgatctg
     2461 gctatgaccc gtttcatcaa agcggcggat catctctgcc aggttatcct gtgacaaatc
     2521 ggattcatat tcatccagaa taacatcagg caaaataaca gctaccggtt catcacccac
     2581 taccgggtga gcacacaata ccgcgtgtcc caggcctttc gccagaccct gacgaacttg
     2641 cataatagtc acgtgcggtg gacaaataga ctgcacttca tcaagcagtt gacgttttac
     2701 acgtttttcc agcattgctt ccagttcaaa actggtatca aagtggtttt caatagagtt
     2761 tttagatgag tgtgtaacca gcacaatttc agtaatgcca gccgcaatac attcattcac
     2821 gacgtattga attaatggct tatcgacaag tggcagcatc tctttcggga tggctttcgt
     2881 cgccggcaac atcctggttc ctaatcccgc aacggggata acggcttttt tgactttcgt
     2941 attaatggca gccatttaaa ttctcctgga ctgttcgtgt tttgaacgtg ttcatatttc
     3001 tgtatcgcat cccagtatat cagcaccccg aaggggctaa gttctgaaaa ggaggcgagt
     3061 tagcataatt aagacaatta cttatacgaa tggcgacgat agtaccacca gccgacatta
     3121 gcaggtaatg caaatttagc ccgcgttatc gtttgctcat tctgcagaca acatcaagcg
     3181 cagtcgacca ccggttcccc atatttggca ttgccaggca tcgcatcgct ggctcaattg
     3241 attcaaataa gcgttaccta aagtgcctaa cggaacacca ttactgattt gcacctggtg
     3301 ttcgccggta tttaacgtcg cattcagacc cgcagaaacc agaatgagat ttttcagttc
     3361 gcgatgataa tagccaacta atagcggaaa ctgccccggc agattggcct gacggagtaa
     3421 atggtttacc tgcttcaata acgcgcccaa ctctggcaac cgttgatttt ggtgtgcaag
     3481 ctgttcctgt aataatccgt taaacaatgc gcgtaataac aaggcagcaa gtacgccatt
     3541 atgtccagct cgggtgacat caaggcaata aaatgccaga tcgttttccg aaagtgcggc
     3601 aatatcaagc accaggccgg gtttgtccgc ggcaaccaat tgacgataat taacccggca
     3661 atgggaaatc acctgctgaa ccggcggttg tagttcctgt aataatttcg ccgctgcggc
     3721 agggttatca accattgcat cccagtcgcg aaaaagcctt tcctcttcct caacgcgcga
     3781 attaaacatg ctgggataga gacaggcaaa aaccatctcg cgcaagcgat tcagatcttt
     3841 aactggtttc agcaaaacat cttcaacgcc cagacgtaac gctttggcaa tatctgccat
     3901 attttcagtg gcagatatca ccagaactgg ggtctggtcg cctctgttac gtatatgctc
     3961 cagcagttta agcccgttca ttcgtggcat cgcgatatca catatcatca ggtctggagt
     4021 gaaacctccc agcaactcaa gggcatccac cccatcagcc gccagtaccg ttgtcgctcc
     4081 caatgaggaa aaccatgaat ccagaagcga gcgaaatacc tgctcatctt caacaatgag
     4141 aatctgtttt ccgaccaatg gctgcgtcat gttctctccc ctgactggct ttactcaata
     4201 gtggcatgct attgccacct gcgcctgtca gaattaactt aagtgtagat aaaaattctg
     4261 gtcaaatgtt ggtgcgtacc aacggcaaaa gttcgtccat tttcctttcc actgccagct
     4321 gtccggccgc aatggcagcg tgcgcgcgat ggaaatcaag cgtagatatt tgcgggcaaa
     4381 caggttgaat cagaatatcg ggcggatcac ctgccatgcg gttcctttta aggcggttct
     4441 ccaacacctg gatagacgtg gtcataatct ctgttgccgt tggcgctgtc acagcccgac
     4501 gtgtcgttat gctgcctaac ctttctttca gacgcgcatg ccacggtaga gaatcaccat
     4561 tctcgctatt ttcttcactg acattaaagg agagcagatc ttgttgcatc aaatgagcat
     4621 cgtgctgcag gtcgaccgct atcacaatat cagcccccaa tgcacgcgta agagaaatag
     4681 gaattgggtt aactactgcc ccatcaacca gccagtagcc gttatgtgca acgggtgcca
     4741 tgagtcctgg aatactgcat gatgcgcgaa tagcaagatg gagatcgcct tcagtaaacc
     4801 ataattcacg ccccgtactt aaattggtgg caacagccgc aaagcgacgg gaacaatttt
     4861 cgatctctgt ttccggcatt atttcgcgat attgattgaa gacacgctcg ccgcgcagta
     4921 acccaccgcg ctgccaggag aggtccatca ggcgtaaaac gtcccaataa ctgaaagagg
     4981 ttacccaatc ttccagcgca gataatcgat cgcatgcata ggccgcgccc accagcgaac
     5041 caattgaaca tcctgcaacg atatcaattt caatacccac tttttttagc gcattaataa
     5101 cgccaatatg cgaccaacct ctcgccgcgc cagatcccag cgccagccct atttttatct
     5161 ttctcattat tcccgcaaga ttcccctgga atgcaatcgt agccacatcg ctactgctcg
     5221 gttaacatag tgctactcag gcggttaata cgccgttatt gtttccaggg agattatttg
     5281 tgtctcagct ttgtccctgt ggtagtgctg tcgagtatag cctatgttgc cacccttatg
     5341 tgtctggtga aaaggttgca cctgatccag aacatctcat gcgttcgcgt tactgcgctt
     5401 ttgtgatgca agacgcagat tatttaataa agacctggca tccctcttgt ggagcagcgg
     5461 cgttacgtgc cgaattgatg gccggatttg cgcatactga atggttggga ttgaccgtat
     5521 ttgaacattg ttggcaggac gccgacaata tcggatttgt cagttttgtt gctcgattta
     5581 ccgaaggtgg caaaacaggt gcaataattg aacgttcgcg atttttgaaa gaaaacggtc
     5641 aatggtacta tattgacggt acacgtccgc agtttggtcg caacgatccc tgcccttgtg
     5701 gttcaggtaa aaaatttaaa aagtgctgcg gccaataatg gttgacggta cggtttagca
     5761 aacactctca acaaggtttt tccagcaatg cattcactcc aacgtaaagt tctgcgtact
     5821 atttgtccgg accaaaaagg tctgatcgca cgtattacca atatttgcta caagcacgag
     5881 ttaaatatcg tacagaacaa tgaatttgtt gatcaccgta ccgggcgctt ttttatgcgc
     5941 acggaactgg aagggatttt taatgattcc accctgctgg cggatctcga tagcgcattg
     6001 ccagaaggct ccgtgcgtga gctgaatcct gccggtcgtc gccggatagt gattctggtc
     6061 actaaagaag cgcattgcct tggcgatttg ttgatgaaag ccaattacgg cggcctggat
     6121 gtcgaaatcg cggcagttat tggtaaccac gatactttac gttctctggt tgagcgtttt
     6181 gatattccgt ttgagctggt aagccatgaa gggttaagcc gcaacgagca cgatcaaaag
     6241 atggcggaag ccattgatgc ttatcaacct gactatgtgg tgctggcgaa gtatatgcgg
     6301 gtattaacgc cggaatttgt ggcacgcttc ccgaataaaa tcatcaatat tcaccattca
     6361 ttcctgcctg cgtttatcgg cgcacgtcct tatcaccagg cctatgaacg tggtgtgaag
     6421 attattggcg caaccgctca ctatgtgaat gacaatctgg acgaaggccc aatcatcatg
     6481 caggacgtta ttcatgtcga tcatacctac acagctgaag atatgatgcg cgcaggtcgt
     6541 gacgtcgaga aaaacgtctt aagtcgtgcg ctatacaaag tactggcaca gcgcgtcttt
     6601 gtttacggta atcggacgat tattctttaa tcgccagcaa aaataactgg ttacctttaa
     6661 tccgttacgg atgaaaatta cgcaaccagt tcatttttct caacgtaaca ctttacagcg
     6721 gcgcgtcatt tgatatgatg cgccccgctt cccgataagg gagcaggcca gtaaaaagca
     6781 ttaccccgtg gtggggttcc cgagcggcca aagggagcag actgtaaatc tgccgtcatc
     6841 gacttcgaag gttcgaatcc ttcccccacc accatcactt ttcaaaagtc cgtaaactct
     6901 caaacgaatc cgcaattaaa tattctgccc aagcggggaa ggatgagaag cttcgaccaa
     6961 ggttcgactt gagcgccagc gagagagcgt tgccgcaggc aacgacccga agggcgaagc
     7021 gcgcagcgct gagtaatcct tcccccacca ccatcgcttc cttaaataat caaaacctgt
     7081 tactccgagc taattttgcc cgatggcacc acgtttaccg ggcctacaat atcctcccct
     7141 gtaggtcgga taagcgaagc gcatccgaca actcaccccg caccgtgccc ataaaaaaac
     7201 cccgccgaag cagggtctat gtgaactaaa attcgcttag tgacgagcgc gcaccagctg
     7261 gtacttacgt gtcagatact ccaccggtac gctccagatg tgtaccagac gcgagaacgg
     7321 gaacagcagg aataacgtca tccccagcac caggtgcaga cggaagataa acgccacacc
     7381 atcgaggtgt tgagaagcgc caccgtggaa ggtcaccacc gactgcgccc agccaaccag
     7441 tttcatcatc tcgctaccgt ccatatgctg agcggagaac ggaatggtca acaggcccag
     7501 cgcgcactgg ataacgagca gcgacaggat caggatatcc gctccggtag tggttgcacg
     7561 cacgcgtggg ctgaacagac gacgcttcag caacagcacg ccgccaatca gacacagcac
     7621 gccgctggca ccaccagcaa acatcgccat tttctgtttc acttcaatcg gcagccaggc
     7681 ttcatacatc cagtgcggcg tcagcatacc gaagaagtga ccaacaaaaa tccccagaat
     7741 accgatatgg aacaggttcg acgccaggtt catccctttg cgatccagca tctggctgga
     7801 cgccgcgcgc caggtgtact gcccgtagtc ataacgcagc cagctaccaa tcaggaagac
     7861 cgccccggct atgtacgggt agatatcaaa gaagaacata ttcaggaatt gcattagtgc
     7921 tgtcctccgg tggtgatatt cagatattgc ggcgcgacgg caccggcaaa gcgacgctga
     7981 tgagcagtga ttgctgaatc accgcagcct ttgtcagcaa agaatttaac ctgctcttct
     8041 tcccagacag catccagcgc ctgcggcgta tcatcgcgcg cttcgtcggc aattttttcc
     8101 gccactttgt cgctgtcgat agcggtattg gccaatttca gcagcagatc aaacatcacg
     8161 gcataacggc tttcacgctg ttgcagacgc gcgctcagca atgccagaat cggcgcgata
     8221 tctttcaaac cttccacggc ttcgctttgc ggcagctgcg acaggtactc cagatacagc
     8281 ggcagatggt ccggtaattc gcggctgttt aactgcaagc cgtgctgctc gtactgcgcc
     8341 agcaggtcca ccatcgcctg gccgcggtca cgggattcac cgtgaacatg ttcaaatagc
     8401 aacagtgacg tggcgcgacc acggtcgaac agttcgctgt actgggcctg ggcatcgagc
     8461 ggatccatcg ccgttaaatc gcgcaggaaa atgcccagcg catgggcatc ttctttcgac
     8521 agatttttcg acgcagcaat cgcctcaaac atctcttgtt gatgctgcca taaggcagca
     8581 tccggatatt caaggagacg cgatacaatc acgagttcga tcatggatgc ggctccgttt
     8641 tgctggtcac atcgatggca tcgatacgac ggctgttgaa cagattgaat ttggtatctg
     8701 aaccgtggca gccatcacca aaggtaaagc cgcagccatt tttctccggg aaggcttccc
     8761 gtgccagttc acgatgacta ctcggcacca caaagcgatc ttcgtagtta gcaatcgcca
     8821 gataacggta catctcctgt gcctgggctt cggtcagacc gacctcttcc agcgcacggg
     8881 tatcaacttt accgtcaacg gtttcagcac gtttgtaatg acgcatcgcc agcatacgtt
     8941 tcagtgcgcg cagtaccggt ttggtatcac cggcggtcag cagattcgcc agatactgta
     9001 ccggaatacg caagctttcg acgtctggca gaatgccgtt gctacccagc tcacccgcgt
     9061 ctgctgcaga ctgaatcgga gacagaggcg gcacgtacca gaccatcggc agtgtgcgat
     9121 attctggatg cagcggcagc gccagtttcc attccattgc cattttataa accggcgact
     9181 gctgtgcggc ttcaataacg ctcagcggaa taccgtcttt aatcgcctgc tcgatgactt
     9241 tcggatcgtt cggatcgagg aacacctcca gctgacgctg gtaaagatct ttctcgttct
     9301 cggtgctggc tgcacgttca atcgcgtcgg catcgtacag cagcacgcca agataacgga
     9361 tacgaccgac acaggtttct gagcacacgg tcggctgacc cgcttcaata cgcggatagc
     9421 agaagatgca cttctcagac ttaccgctct tccagttgaa gtagattttt ttgtacgggc
     9481 atccggtgat gcacatacgc cagccacggc atttatcctg gtcgatcagg acgatgccat
     9541 cttcttcacg cttgtaaatc gcaccgctcg ggcaggtcgc cacacatgcc gggttcaggc
     9601 agtgttcgca caggcgcggc aaatacatca taaaggtgtt ttcgaactgg ctatacatcg
     9661 ccttctggat gttgtcgaag ttcttgtctt tcgccagttt gtcaaactca ccacccaggt
     9721 catcttccca gttcggccct ttttcgattt tcgccatccg ttccccggta atcagcgaac
     9781 gcggacgggc aatcggctgc gatttgctgc cttccggcgc ggtatgcagg ttctgatagt
     9841 caaaatcgaa cggctcgtaa taatcgtcga tccccggcag atgcgggtta gcgaagattt
     9901 tacccagcaa catggcacgg ttacccatgc gcggctgcag tttgccgttg attttacgga
     9961 tccagccgcc tttgtatttt tcctggtttt cccagtcagt cgggaagccc tgacccggct
    10021 tggtttccac gttgttgaac cacgcgtatt ccacgccttc acggctggtc cagacgtttt
    10081 tacaggtaac tgaacaggtg tggcagccga tgcacttatc gagattcagc accatgccga
    10141 cttgtgaacg aattttcatt ttacgctctc ctgtacctgg tcattgcctt cgccatctaa
    10201 ccagtcaatg ttcttcatct tacgcactac aacaaactca tcgcggttag aacctacggt
    10261 gccgtagtag ttaaagccgt atgccagatg ggcatagccg ccgatcatat gcgtcggttt
    10321 cggcgtgata cgggtgaccg agttatggat accaccacgc tgttgggtaa tttccgaacc
    10381 aggcaggtta acgatacgtt cctgcgcgtg gtacatcatg gtcatccctg ccggaacacg
    10441 ctggctgaca accgcacggg cagtcagagc accgttgctg ttgaatactt caatccagtc
    10501 gttatcggcg atacccagat ctttggcatc ggcttcactc aaccagacca ccggaccacc
    10561 gcgacctaaa gtcagcatca gcaggttgtc gctgtaggtg gagtggatac cccacttctg
    10621 gtgcggcgtc aggaagttga gcgctttttc cgggttgccg ttggatttct ggcctatcac
    10681 ttctttcacc gaacgggtgt cgatcggcgg acgataaacc agcaggcttt caccgaaatc
    10741 acgcatccac tggtgatcct gatacagttg ctgacgacca gagagcgtac gccatgggat
    10801 cagctcgtga acgttggtgt aaccggcgtt gtaagaaacg tgttcatctt ccagaccaga
    10861 ccaggtcggg ctggagataa ttttgcgcgg ctgtgcctga atatcgagga agcggatctt
    10921 ctcgtcttct ttattcagcg ccagatgcgt atggtcacga ccggtaaatt cgctcagggc
    10981 agcccaggct ttcacggcta cctgaccgtt ggtttccggt gccagtgtca ggatcatctc
    11041 tgccgcatca attgcggtgt tcagcatcgg ctggcctttc gccggacctt ccgctttggt
    11101 gtagttgagc ttacgcagca gatccatctc gctctgggtg ttccaggcaa tccctttacc
    11161 gccattaccg attttctcca tcaacgggcc aatagaggta aagcgttcgt aagtcgccgg
    11221 ataatcgcgc tctacgacca taatgtgtgg cgcggtttta cctgggatca ggtcgcactc
    11281 gccttttttc cagtctttca catccagcgg ctgcgccagt tcagcggcag agtcatgctg
    11341 gataggcagc gtgacgatgt cggtttcttt acccagatgg ccgacgcaca cttcggagaa
    11401 tttcttcgcg atggctttgt agatttccca gtcgcttttc gcttcccagg ccggatcgac
    11461 cgccgcagac agcgggtgaa taaacggatg catatccgaa gtattcatgt cgtctttttc
    11521 gtaccaagtc gccgtcggca aaatgatgtc ggaatagaga caggtgctcg acagacggaa
    11581 gtccagcgta accaccagat ccagcttgcc ttccagacca ttgtcctgcc agtccacttc
    11641 ttccggcttc acgccgccct gttgccccag atctttaccc tggataccgt gctccgtccc
    11701 cagcaggtac ttgagcataa actcatgacc tttaccggaa gaaccgagca ggttagaacg
    11761 ccagatgaac aggttgcgcg ggtggttttt accgttttct ggttgttctg ccgcaaaacg
    11821 gatggaaccc tctttcaggg atttcaccgt atagtccacc ggattcatcc cggctttttc
    11881 cgcttcgcca gcgatagtca gcgggttagt gcctaactgc ggtgcagacg gcagccagcc
    11941 catgcgttcc gcacggacgt taaagtcgat caagtgtccg gtatagcggg atttgtccgc
    12001 catcggtgac agcaactctt ccgccgtgac ggtttcataa cgccactggc tggagtggtt
    12061 atagaaataa gaagtgctgt tcatgtgacg cgccggacgc tgccagtcaa gggcaaacgc
    12121 cagcggctgc cagccggttt gcggacgcag tttttcctga cctacatagt gcgcccagcc
    12181 gcccccgctc tgaccgacac agccgcagaa aatcagcatg ttgatcagac cacgatagtt
    12241 catatcgagg tgataccagt ggttcagccc cgcaccgacg ataatcatcg aacgaccgtg
    12301 cgttttatca gcgttatcgg caaattcacg ggcgatgcga ataatctggc tgcgagaaac
    12361 gccggtaatc tgctcggccc aggccggggt ataagctttc acatcgtcat agctggttgc
    12421 acagttaacg tcgttcaggc cacgttccag accgtagttt gccagcgtca gatcataaac
    12481 ggtgctcacc agggcggtgc tgccatcagc cagttgcagg cgtttcaccg gcagtttgtg
    12541 cagcagcacg ttttccagtt ccactttgtt gaagtgttcc gtgccgtcgc caccaaagta
    12601 cgggaagcct acctcagcga tctcatcctg gctacccagc aggctcagtt gcagctcggt
    12661 ttcttcgcca gttttgccgt cgcgctgctc aagattccat ttgcccttct cgccccagcg
    12721 gaagccaata gaaccgttcg gcgcaaccat ttcgccattg gtattaaagg cgacagtttt
    12781 ccattccgga ttgttttcct ggcccagcgc atcaaccaga tcagcagcgc gcagcatacg
    12841 acctgcagcg tagtaaccgt cgcgttcttc cagcatcacc agcatcggca tgtcggtgta
    12901 gcgacgcaca tagtcggtga aatactggct tgggttgtcg aggtggaatt cacgcagcat
    12961 tacgtggccc atcgccagcg ccattgccgc atcggtgccc tgtttcggtg ccagccacag
    13021 atcgcacagt ttggcgattt cagcgtagtc tggtgtgacg gcaacagttt tggtgccttt
    13081 gtaacgcact tcagtaaaga agtgagcatc cggggtacgc gtctgcggca cgtttgaacc
    13141 ccaggcgatg atgtagctgg agttatacca gtcagcagat tccggtacgt cagtttgctc
    13201 gccccaggtt tgcggagacg caggaggcag gtcgcagtac cagtcgtaga agcttaagca
    13261 agtaccgcca atcagcgaga gatagcgtgc acccgatgcg taagaaacca tcgacattgc
    13321 cggaattggc gagaaaccag caacacggtc cgggccgtag tttttgatgg tgtaaacgtt
    13381 agatgcggcg atcagttcgt tcacctcctg ccaggaagaa cgaacaaatc caccgcgtcc
    13441 acgcgcctgc ttaaagcttt tcgctttatc ggcgtcttca atgatagaag cccatgcctc
    13501 aaccggatcg ctatgcagcg ccttcgcttc acgccacatt ttcatcaggc gtttgcgcat
    13561 catcgggtat ttcaggcggt tggcactgta aagataccag gagtagctgg caccgcgcgg
    13621 gcagccgcga ggttcatggt ttggcagatc cggacgggta cgcggatagt cagtctgctg
    13681 ggtttcccag gtgaccagac cgtttttgac gtagattttc cagctgcagg agccggtgca
    13741 gtttaccccg tgggtagagc ggacgatttt gtcatgctgc caacgctggc gatatccatc
    13801 ctcccagtca cggttggtat tgagaagctg gccatgccca tcggcaaagg tttcaccctt
    13861 ctgtttgaag tagcgaaacc ggtccaggaa tttactcatc ggttttctcc tgtgggagcc
    13921 tgtcggccct ctgataaatc gacattgcta atgtttaagg gcaaggtaac gctctgaaac
    13981 agcgtgggaa ttgataacga tcaagagtga tggggatgaa aaataaagta aattcctaag
    14041 ataccccttt aggataatta acagccacca cataacagac tgtaaattaa gacgttttat
    14101 acctgtagtt atttaaacta ttaagatttt tatgcagatg ggtattaagg agtattcccc
    14161 atgggtaaca tattaatggc tcttaatgaa atccggcgaa ataagccata taatatgagt
    14221 atgttgtaac taaatgacgc ataaaaaaag cgcgacatca tgccgcgcaa aggataatca
    14281 aatattaaga tttatttttt agaatgccga ccatataccg cccaggtaat cactacgcag
    14341 gcgatataga aaatcaaaaa taccttcatt gcgccgactg gcgaacccgt taatgccagc
    14401 gagctaccaa acgctttcgg gataaagaag ccaccaatag cgccaatcgc agagatgaaa
    14461 cccagcgccg ctgccgtgtc ggttgccgct tcacgcatcg cacgttcgtc agaaccccct
    14521 tctgctttca cgcgatccat tgtcagttta cggaagatca ctgaaatcat ctggaaagtg
    14581 gaaccactac ccagcccagc tgtcaggaac agcgccagga agaccgcgaa gaacgccatg
    14641 aagcttccgc cctgcccgtc agtcggtaag gtcaggaaca gcaggccgct gaaaatcgcc
    14701 atcagaataa agttcaccag cgtgacacga gttccgccca gacggtcaga taatgcacca
    14761 cctgcagaac gcgccagcgc accaataaac ggcccgaaga aagcgtattg cagaatctga
    14821 acatccggga actgcgtttt tgacagcatc gcaaagcccg cggagaagcc gataaaggag
    14881 ccgaaggttg ccagatacag caggctcata atccacagat gaccccgttt gagtaccggc
    14941 aactgctcct tgatggaggc tttcgaggta gcaagatcgt tcatgccaaa ccacgccgca
    15001 atggtgaaga tggcaaggaa cggcacccat atccaggacg cattcgccag atacagctca
    15061 gtcccatccg gctgtttgac gccctggcta ccaaatactg cgaaaatcga cagtgatacc
    15121 accagcggag caaccaactg catgacgctg acgcccatgt ttcccagacc accattcaga
    15181 cccagcgcgc caccctgctt ctgtttcgga aagaagaagc tgatgtttgc catactggat
    15241 gcgaagttcg cgccagcaaa gccgcatagc agagagatga tgatgaagac gctataaggc
    15301 gtggaggtat cctgcacggc aaaacccagc cagacgcaag gaataatcag aataccggtg
    15361 ctgaacgccg tccagcgacg accaccgaag ataggaacca taaaggagta tggaacacgt
    15421 aataacgcgc cagaaaccga aggcagcgca gtcaacataa atagctgatc ggtcgtaaaa
    15481 ttaaaaccga ctttcggtaa gttcaccgca acagcgctga acaacatcca tacgcaaaac
    15541 gccagcagca gacagggaac ggaaatccac aggttacggc tggcaatacg ttgaccgcgt
    15601 tgttgccaga acgcaggatc ttccggtcgc caatctgtaa tgacagctcc agtagccctt
    15661 tcgggggcgg atgagtgact catagacacc tctgatactc gtttcgatgt ctgccacctt
    15721 agtgtctgta gctaaaggca atttgatgta aatcaaacga taagataact ttatctatca
    15781 ttgatattta tcattaccca tagtgagtac agtgatttca taaaaattat gagatttttc
    15841 acggtgctgt aaaatcccta cccttaccga tgtaaagcga ctaaccacac ggcaaataag
    15901 gagtaactct ttccgggtat gggtatactt cagccaatag ccgagaatac tgccattcca
    15961 gaatgtatcg tcacattcat taaggttatt gctcatttaa agcctgaagg aagaggtttg
    16021 catgcttaaa cgttgtctct ctccgctcac cctggttaat caggttgcgc ttattgtgtt
    16081 gctttctact gctattggac tggcagggat ggcggtttct ggctggctgg tgcaaggcgt
    16141 tcagggcagc gcccatgcga tcaacaaagc gggatcgctg cgcatgcaaa gttatcgtct
    16201 gttggcggca gtgccattaa gtgagaaaga caagccctta attaaagaga tggaacaaac
    16261 ggcatttagc gccgagttaa ctcgagcagc agaacgagac gaacaactgg cgcaattaca
    16321 gggtttacaa gattactggc gtaatgaact gatccctgca ctgatgcgtg cacaaaaccg
    16381 cgaaacggtg tcagcggatg tcagccagtt tgttgccggg cttgatcagc tggtatctgg
    16441 ttttgaccgc accacggaaa tgcgcattga gacagtggta ctggtccatc gggtaatggc
    16501 ggtatttatg gcacttttac tggtgttcac tattatctgg ttgcgggcgc gactgctaca
    16561 accgtggcgg caactgctgg caatggcgag tgccgtcagt catcgcgatt ttacccaacg
    16621 cgcgaacatc agcgggcgca acgaaatggc gatgcttgga actgcattga acaatatgtc
    16681 tgcagaactg gccgaaagtt atgccgtact tgagcagcgg gttcaggaga aaaccgccgg
    16741 gctggagcat aaaaatcaga tcctctcttt tttatggcag gctaaccgcc gtttgcattc
    16801 ccgcgccccg ctgtgtgaac gcctgtcacc tgtactcaac ggcttacaga atttaaccct
    16861 gctacgtgat atcgaattgc gggtgtatga cactgatgat gaagagaatc atcaggagtt
    16921 tacctgccag ccagatatga cttgtgatga taaaggctgc cagctctgcc cgcgcggcgt
    16981 attacccgtt ggtgatcgcg gcacgaccct gaagtggcgg ctggctgact ctcatacgca
    17041 gtacggtatt ttgctggcga ccctgccgca ggggcgtcat cttagccatg atcaacaaca
    17101 actgctggat accctggttg aacaactcac cgccacgctg gcgctggatc gccatcagga
    17161 acgtcagcaa cagttgatcg tgatggaaga gcgtgccacc attgcgcgcg aactgcatga
    17221 ttctattgcc caatctctct cttgcatgaa gatgcaggtg agttgtttac agatgcaggg
    17281 cgatgcgctg ccagaaagca gccgcgaact gttaagtcag atccgtaacg aactgaatgc
    17341 atcctgggcg cagttgcgtg aattgctcac cacattccgc ttgcagctca ccgagcctgg
    17401 attacgtccg gcgctggagg cgagttgcga agagtacagc gccaaatttg gcttcccggt
    17461 gaagctggat tatcaattgc cgcctcgcct ggtgccttcg catcaggcaa tccacttgtt
    17521 gcaaattgcc cgtgaggcat taagtaacgc cctcaaacat tcgcaagcga gtgaagtcgt
    17581 ggtgacggtg gcgcaaaacg ataatcaggt caaactgacc gtccaggata acggctgcgg
    17641 cgtgcctgaa aatgccatcc gcagcaatca ctacggcatg ataataatgc gcgaccgtgc
    17701 gcaaagttta cgaggcgatt gccgcgtccg ccgtcgtgaa tcaggtggca ccgaagtggt
    17761 ggtcaccttt attcccgaaa aaactttcac agacgtccaa ggagataccc atgagtaatc
    17821 aggaaccggc tactatcctg ctgattgacg atcacccgat gctgcgaact ggcgtaaaac
    17881 agcttatcag tatggcacca gatatcaccg tggttggcga agcgagtaat ggcgaacagg
    17941 gtattgaact ggcggaatct cttgatcccg atctgatcct gttagatctc aatatgcccg
    18001 gcatgaacgg tctggaaacg ctggataaac tgcgcgaaaa gtccctctca gggcgcattg
    18061 tggtattcag cgtctctaac catgaagaag atgtggtcac cgcactgaaa cgcggcgcgg
    18121 atggttatct gttaaaagat atggaaccgg aagatctgct gaaagcattg catcaggcag
    18181 ctgctggcga aatggtatta agcgaagcat taacgcctgt tctggccgcc agcttgcgcg
    18241 ctaaccgtgc cactactgag cgcgatgtta accagttaac cccacgcgag cgcgatattc
    18301 tcaagctgat tgcccagggt ttgccgaaca agatgattgc ccgccgcctg gatatcaccg
    18361 aaagcacagt aaaagtgcac gtcaagcaca tgctgaagaa aatgaagctc aagtctcgcg
    18421 tggaagcagc ggtatgggtg catcaggagc gcattttctg attacggttc ccagcgcagt
    18481 tcgtcgtttg acaatgcatc gaacggttcg acaagcgtta gcgtgatctc attggaggag
    18541 acacgctgcc cctggttatc ttccaccacc accgacaatc gccagtgatt gctcgcccct
    18601 tccccgttct gccagtcagg catgatcagc gtccagccct ccgcgctgtt ggcttgtgcg
    18661 cccggcgtca aacttaatat ctgcgtatcg ccctgccaaa tcagttgccg aataccgtaa
    18721 cgactgcgga tttgtaattt cagcggcact gtttcgccag gttttagatc ccacggcggt
    18781 gtcgccagaa acaccgttaa cgtttttcgc tgtcggtact caagagtcgg tagattattt
    18841 cgctgcggat tatcatagcg actaccacgt aacgactgac tttcggcaac ctcgcccgca
    18901 gaaagttgtt ttttgagcgg cacaccaaag cggtaattaa gattcagtcc gaggttattt
    18961 tgattttcgc cactttcacc ctgtttatgc tgggccgtca cagtgactaa tggcacaggg
    19021 gtgtaattta atcccagact caacgcgacg ggattgtgat aacccgtacc agagttaaac
    19081 aaatcaacac gctcaccaaa atactgttct accctgacac tggtattgag gtgttgatag
    19141 aacggcatgc gcatccgggc tgtcaggtca tacccgcgcg ccatccgttg ttcctgcgtg
    19201 gctgtctgtt catgccatgc agcaaacggc tgataaaagt ttgccgatag tcgcaaatat
    19261 tcgccccacg cttcggcacc aaagcccgct cgctgaagat tttcgtccag caagttgtca
    19321 taaaaagtgt tataacccac cagccagttg ccgcgcgccc agcgttgccc aacgcccaca
    19381 ttgctcacca acccatcatc ctgctgagta agaccaagct ggctccaggt gagataacga
    19441 tcattatctt gtaacggcac aaaccagctt ccacgactgc cggtgaaatg tccttcgtta
    19501 tccactttga cgtcaacact ggcatttccc cacggtgata gccaggactc tacgtgctga
    19561 ttaacctgtt gactaagcgc gtcgcggact tttcccaatg cgaaagcttt tgcctgctcg
    19621 ccagtatcca gcccgttatc attcatactg gtttcgccga aatctttcac aatttcagca
    19681 aagtgttttt ccccatcatg gttttcggga gccatgccta aatccggcag accatcattg
    19741 ttattatcaa agggatttgc cgctttttgc tcgaaggtag attgtgcgtt agctgtaccg
    19801 cctgccgcca gcaagagtaa ataaaacggg ataatgcgag gaacgaaacg gctcaaaacg
    19861 tcggtaactt ccggtaataa tgaaaaacca tagccttaaa gaataacgat tactggcaac
    19921 ttttgtcgta ttttctcgcg ctttcaggaa aatccttaaa aaggtgaccg attggtcaga
    19981 cgctgcagaa gcagtaagat agaagatgag tgtctaattc cctgaataca ggagtaaata
    20041 atgcaaaaaa tcgtgatcgt tgccaatggc gcaccttacg ggagcgaatc cttgtttaac
    20101 agcttgcggc tggccattgc gttacgagag caggagagca atctggatct gcgtctgttc
    20161 ctgatgtctg atgcggtcac agccgggttg cgcgggcaaa aaccagggga aggctacaac
    20221 attcagcaaa tgctggagat ccttaccgct cagaatgtac cggtgaaatt gtgcaaaacc
    20281 tgtaccgacg ggcgcgggat tagcacactt cctcttattg atggggtgga aatcggtact
    20341 cttgtggaac tggcgcaatg gacgctgtca gccgataaag tgctcacatt ttaatgtttg
    20401 agggggcgtc tgacgcccca ctatgaggga agtttactca ggcgaatccc ggccgtaaca
    20461 caccatccgg ataattctcc gccagcagtt tttttaccga taccagcaaa tcattcagcc
    20521 catcgtcctg cattcccagt ttgataagct cctgttccag tgaaaacagg tattgtgcgt
    20581 tggttcccag cggaccgctc gccgccgcaa tcaacggcgc gatgacctga gcgcgagtgt
    20641 cagattcata ttctggatgt cgcgggtcca taataaacac aatggcgttt actgtgcgtc
    20701 catcatcaag atcaagctga caccaggttg gcagataaca gccggtaatc atctcgcgct
    20761 tccacaacag ggttagttcc tgctccagcg tctcttctgg tagtcgatag gcgacgcctg
    20821 tggtgcgtcc gccctctttc agtgcaagca tccgtcccgg ctggtgcgca gtcccgcgcc
    20881 cggcggtcag gcgcaggcaa aacgcgcgat gccatccaac cagtgtaccg gtgcataatt
    20941 cggtaaactc cagtgccgga ttccacatca acgatccata accgaagatc cacaccggtc
    21001 cctcattagg tcgacaagcc agcgtcgccg ccagcgaagc cgcccgttgt tctgctgacc
    21061 ataagagtga ttcttcaatg gcaccaaatg ccgttttaca atcggcattc atcaagaaat
    21121 cacgcgttat caccttacac ctccgccact gcatacttcc ctgcgacaac tttttggtgt
    21181 cttccggaca tttttaccgc tcattttgaa accactaacg ggacaatatg caatccacgg
    21241 accacttgca aggcaacacc gcacgaatag ctttaactaa gctaccggtt ttacgatttt
    21301 ttatgccatt tatcatcatc ccctttggca tattcatgct tcacagcagc ccaggccact
    21361 ttatgcgcgg tttcttcgcg actggcgtca tcacgccgat cttctttatc tttatattga
    21421 tcccatgcgc tgttgaacgc ttctttatag atatcctggg catgagacgg tagaacgtgc
    21481 tttacgcttt ccggcagatc gcttttcgtt ttatacggca tcgttgactc cttttacaaa
    21541 gttgaacatt aagtgtggtc aacaatatct actcttgcca aaacataaga attttcatgt
    21601 cagactgttc ttaagaaacg ctaaaacaaa cattgtctaa acgtaatact tatttttatg
    21661 ccccaatgag taaaaaatcg acagcggggt aaaaattcgt aaaatgttac cgatatccag
    21721 acactttctg ttatttttcc tacctatttt ttacattttc gataaattca tctgcaaagg
    21781 gataaccata atgtcaaatg ctcaagaggc ggtaaaaacc cgccacaagg agacttcgct
    21841 tattttcccg gttctggcgc tggtagtgct gttcctgtgg ggaagcagcc agacactacc
    21901 agtggtcatt gccatcaatc ttcttgcgct tattggtatt ttaagtagcg cctttagtgt
    21961 tgtccgtcat gcggacgtat tagcccatcg cctgggagaa ccttacggtt cgcttattct
    22021 tagcctttca gtggttattc ttgaagtcag tttgatttca gctttaatgg caaccggcga
    22081 cgccgcgcca acgctaatgc gtgatacgct ctattcaatc attatgattg ttaccggtgg
    22141 gctggttggc ttttcattat tgttgggcgg tcgtaagttt gccacccaat atatgaatct
    22201 gtttggtatc aagcagtatt taattgcgct gttccccctg gcgataatcg tactggtatt
    22261 tccaatggct ctgcctgcgg cgaatttttc aaccggtcag gcgttactgg tagcattaat
    22321 ttctgcggca atgtatggcg tatttttgct gatccagacc aaaacgcatc aaagtttgtt
    22381 tgtctacgag cacgaagatg acagtgatga cgacgacccg caccacggta aaccgtctgc
    22441 ccatagcagc gtgtggcatg ctatctggtt gattatccat ctgattgccg ttattgcagt
    22501 caccaaaatg aatgccagcc cgctggagac attgctcgac agcatgaatg cccctgtcgc
    22561 ctttactggc ttcctggtgg ctctgttgat tctgtcgccg gaaggtttag gtgcgttaaa
    22621 agcagtgttg aacaaccagg tccagcgcgc aatgaatctg ttctttggtt cagtgttagc
    22681 aaccatttcg cttaccgtac ctgtcgtcac gctaattgcc tttatgacgg gtaacgaatt
    22741 acagtttgca cttggtgcgc cagaaatggt ggtgatggtg gcctctttag tgctgtgcca
    22801 tatctccttc tccaccggac gtactaacgt gctcaatggc gcagcgcatc tggcactgtt
    22861 tgccgcctat ttgatgacga tatttgcctg acagttatat ctcctccgat gacggaaatc
    22921 gggggagata agataaattc ctgcttattg tatttcttca tactacctgg acagctcgtt
    22981 aatcagaatt gcgagcctgt accacaacct gcacgaaacc actgcaacag ctgttgaaaa
    23041 gccggaatgt actctttatt gcaaacattt cagggatgaa gtgaagatga attgagacgt
    23101 agtatccaca ccaagccgga attatccggt gaggcgcaat gttgcggggg ctttatccct
    23161 ggtggcattg gttgctggaa agagaaaacc cccgcacgtt gagaggtaaa acctgacaac
    23221 aaaacggggg ctaatcttga aaccagacac ttcaagaata gccgccttgt tgtcattaca
    23281 acaaggcggc tatatgacgc tcgcgcagtt tgccatgact ttctggcacg acctggcagc
    23341 accgatcctg gcggggatta ttaccgcagc gattgtcagc tggtggcgta accggaagta
    23401 aggtgaaaac gggcgtcagg ctgccgtgat ggcaatatgc gcccgaaacc ggccgcaggg
    23461 gtaacagcct gcggccattt ttgtctttat ctcttccaca agtaaatatt ggcaacccga
    23521 gctggaagca tcatccagag cactcatgaa acgactgtaa cagctgttga aaaaccggaa
    23581 tgcactcttc attgcaaaca tttcagggat gaagtgaaga tgaattgagt cgtagtatcc
    23641 acaccaagcc ggcgttatcc ggtgaggcgc aatgttgcgg gggcttaatc cctggtggca
    23701 ttggttgctg gaaagagaaa acccccgcac gttgagaggt aaaacctgac aacagaacgg
    23761 gggctaatct tgaaccatca acaattcaag aatagccgca aaatgttgtc attacaacaa
    23821 ggcggctata tgacgctcgc gaagtttgcc atgattttct ggcacgacct ggcagcaccg
    23881 atcctggcgg ggattattac cgcagcgatt gtcggctggt ggcgtaaccg gaagtaaggt
    23941 gaaaacgggc gtcaggctgc cgtgatggca atatgcgccc gaaaccggcc gcaggggtaa
    24001 cagcctgcgg ccatttttgt ctttatctcc tccacaagta aatattggca acccgagctg
    24061 gaagcatcat ccagagcact catgaaacga ctgtaacagc tgttgaaaaa ccggaatgca
    24121 ctcttcattg caaacatttc agggatgaag tgaagatgaa ttgagacgta gtatccacat
    24181 caagccggcg ttatccggtg aggcgcaatg ttgcgggggc tttatccctg gtggcattgg
    24241 ttgctggaaa gagaaaaccc ccgcacgttg agaggtttaa cctgacaaca gaacgggggc
    24301 taatcttgaa ccagaacaat tcaagaatag ccgcaaaatg ttgtcattac aacaaggcgg
    24361 ctatatgacg ctcgcgcagt ttgccatgat tttctggcac gacctggcag caccgatcct
    24421 ggcgggaatt attaccgcag cgattgtcgg ctggtggcgt aaccggaagt aaggtgaaaa
    24481 cgggcgtcag gctgccgtga tggcaatatg cgcccgaaac cggccgcagg ggtaacagcc
    24541 tgcggccttt tttgtatctg gcacccatga aaaaagtctt aacgcagaac gctaaggctt
    24601 tatttttcaa gcaaaaaaga ttacttgctg gtatccagtt cttcgaaacc tttcaccaga
    24661 tcatcaatcg ctttcatctg cttgaggaac ggttccagtt tagccagcgg cagcgcggat
    24721 ggaccatcac atttcgcatg ttccggatcc ggatgcgctt caataaacag ccccgccaga
    24781 cctaccgcca taccggctcg tgccagctca gccacctgag cacgacgacc accggaagct
    24841 gcgccaaacg gatcgcggca ttgcagtgcg tgggtcacgt cgaaaatcac cggcgagtta
    24901 ccagacactt tcttcataat gctgaagccc agcatatcga caaccaggtt gtcatagccg
    24961 aagttagcac cgcgatcgca aagaatcact ttttcgttgc cgccttcttt gaatttatca
    25021 acgatattac ccatctgtcc cgggctgaca aactgtggtt tcttgacgtt aattaccgca
    25081 ccggttttcg ccatggcttc aaccaggtca gtctggcgag caagaaacgc cggcaactga
    25141 atcacatcca cgacatcagc aacgggctgt gcctgacttg gttcgtgaac gtcggtgata
    25201 attttcacgc caaaagtctg cttcaactcc tggaagattt tcatcccttc ttccaggccc
    25261 ggtccacgat aagagtggat ggaggagcgg ttggctttgt caaaagaggc tttgaacacg
    25321 taagggatac ccagtttctg ggtcacggtt acgtagtgct cgcaaatgcg catcgccaga
    25381 tcgcgagatt ccagcacgtt cataccacca aacagtacga acggcaggtc atttgctacg
    25441 ttgatgtcgc caatgctaac cactttttgt ttcataggat cgccttaatc ttgagtaaaa
    25501 tgtcgattaa ttaatgcagc acaatatgtt tatgcgcgat gttatttatt tgcgcacgga
    25561 tcatttcgct gatcgggtct tccggacact gttcaacgaa ataacttaaa tcgttcaacg
    25621 caacgtgttc gcaatccagt tgcgcataaa tcaacccgcg atcgcgaatt tcatagggat
    25681 cttccgggtt gaattgtaat aaagcttcgc tggtgcgtaa cgccagctcc atctgatttt
    25741 cttccatcaa cgaggctttg agtgtatcca acaatttgcg gattacctca atgttatcag
    25801 cttcatcaag gtcttcataa aacagttccg ccgacgggct gatatttccc tttaaccata
    25861 cgtccagcat atgttcgctt aacgattcac cgttaaaagg attaatcagc caaatttcgc
    25921 catccggaca ttcaatgcgc aatatcagct gcgtagggaa aatcaccggc agcagcggca
    25981 aatcgagacg attcgcgacc cataataaaa ccgcacctaa tgataccgca ctgcctgtcg
    26041 attctttaac acctggtcca gccataatgc atcggaaaga cgataaacgc cgcgtgaggc
    26101 tttaaatccc cagtcgccgt aaaacagcgc gatcagtttt tccagctgct cttctaaagg
    26161 cagaagctgg ctgatttctt ccttcgccag actaacgaga cgctccagtt cgtcgtaaac
    26221 atcttgcgag ggaaaatcgc ggcggattgc ttcgcaagcc aggatcatgc cttcgcacaa
    26281 tggcgcttta ttaaattcga aatcagctaa cgatctcatg acttacccca gtaacggtac
    26341 ttttgtggtg gcgagtttaa tgatgatgta cagcaccacc aacgccagcg ggaaggcaat
    26401 aatgcgcgcc tgctgactgt gcatacgacg ataatcgagt gcaataaaac ccaaaacgat
    26461 ataaatgata actccaaaca gcttttcagt cagccactgt gcctgcccgg aaaatggcag
    26521 gatgtgcgct ttagccatca acgcaatgcc gcttaacaat aacaccgtat cgacaaccgg
    26581 cggcacaatg cgcgtccagc gagcaaatgc ctgtggatgc ttctgataac gtagccagaa
    26641 acgtaaggtt aatagcccaa cagaaagcgc gatactaata agatgaacac taagcagtgt
    26701 agaaaaactt gtcattgata atagcggccg agcgttacgc gctcgttatc accatagtca
    26761 cggcaggttt cgacgtcatg ataccccgcg aggataaatg cttgtcgcac cgcttcgccc
    26821 tgctgccagc catgttccag aagcagaaag ccgccggata ccagcgcgtt acgcgactgt
    26881 tcgatgatat gcacgatgtc tgccattcca ctgtctgccg caaccagcgc agtgagcggc
    26941 tcaaagcgga catcgccttg ttgaagatgt gggtcctgct cgtcaatata cggcggattg
    27001 ctgacaatca tcgcaaactg ctgcccggct agcgcgctaa accagtcgct ttgcagaatg
    27061 tggatatttt tgatcgccag atgctgggca ttacgttgtg ccagggagac agcatcaggc
    27121 atacgatcta cagcgataat ttcgcagtcc gggcgctcgc tagccagcgc aagcgcaatc
    27181 gccccggtac ccgtcccgag atcgagaata cggcaaggtt gttcaggcaa ccgcgccagt
    27241 gcctgctcca ccagacactc cgtatccggg cgcggaatta aggtcgctgg cgaaacaaat
    27301 aacggcaacg accagaattc tcgcaccccg gttaaatgag caatgggttc accatcgcga
    27361 cgacgtgtca gtagcgcatc aagttgctga cattgttcgt cagtcagctg cgtttcacca
    27421 aaggccagaa taaaagtccg ccccctgcca gtgacatact ccaataagat ttcagcgtca
    27481 cggcgtgggc tttcgctcgc ctgaagttgg cttattgctt cacgtaacca gtattgatat
    27541 tccattattc ctgctcggac aacgccgcca gttggtcggc ctgatgttcc tggataatcg
    27601 gttcaatcag catatccagc ttaccttcca tcacttcatc cagacggtag agcgtcaggt
    27661 tgatgcggtg atcggtaacg cgcccctgcg ggaagttgta agtacggtta cggtcgctgc
    27721 gatcgccact ccccagcagg ttacgacggg tagacgcttc ggcctgttgc cgttttgcca
    27781 tttctgcagc gtggatacga gcaccaagta cagaaagtgc tttagctttg tttttatgtt
    27841 gtgaacgttc gtcctgacat tcaacaacaa tcccggtcgg caagtgagta atacgaattg
    27901 ccgaatcggt ggtgttaacg tgctgaccac ccgcccctga cgagcggaaa gtatcaatgc
    27961 gtaaatctgc tgggttgatg tccggcagtt ctgcgtcagg cagttctggc attaccgcaa
    28021 cggtacaagc agaagtatga atacgaccct gcgattccgt agcaggaaca cgctgcacgc
    28081 gatgaccgcc ggattcaaat ttcagacgac catacacacc atcaccgcta attttggcga
    28141 tgatctcttt ataaccacca tgttcaccct cgctggcgct catgatttct acccgccagc
    28201 ggcgggcttc ggcataacgg ctgtacatac ggaacagatc gcccgcgaac agcgccgctt
    28261 cgtcgccgcc ggttccggct cggacttcga ggaaggcgtt acgttcgtca tcaggatctt
    28321 ttggcagtaa cagaacctgt aattgctgtt ccagttgctc gcttttttct ttagcttcgc
    28381 gcagttcatc ctgcgccatc tcacgcattt caggatcatc gagcatcatc tgtgcggttt
    28441 cgatatcttc ctgaacctgt tgccagtcgg taaaacagcg cgaaacatca cttaactgcg
    28501 catattcgcg tgataatgcg cgaaaacgtt cctggtcggc gatagtttgc gcgtcaccca
    28561 gcaacgcctg aacttcttca tggcgttcat gcagggcttc cagtttggca acgatagaag
    28621 gcttcatagg cgtaaatgca ccctgtaaaa aaagaaaatg atgtactgct actccagccc
    28681 gaggctgtcg cgcagaatat tcaggcgttc gttatccccg tcacgggcgg cctgttgaag
    28741 tgatttcgtt ggcgcatgga tcaagcggtt agtcagtttc catgccagat cctgcataat
    28801 ggcttgcgcg tcgccgccct gctcaagggc cgctaacgct ttggcggtta actcatcgcg
    28861 aacgtgctct gcctggctgc gatactcgcg aatggtttcg ctggcgcttt gtgctcgcag
    28921 ccacgccata aattcgctgg tttcctgagc gacaatagtt tccgcctcaa ccgctgcggc
    28981 tttacgctgc gccaggttgt gcgaaatgat gctttgcaga tcgtccacac tataaagata
    29041 agcattcgcc agtttgccaa cttccggctc aacatcgcgc ggaacggcaa tatccaccaa
    29101 cagcattggt tggttacgac gactttttaa tgcgcgctcc accatgcctt tcccgataat
    29161 cggtaacggg ctggcggtgg aactgatgat gatatcggct tcgcgcagac gttcgtcgat
    29221 ctcactcagg gcaatcactt ccgcgccaac ttcatctgcc agaatttggg cacgttcgcg
    29281 agtgcggttg gcgataatca tcttctgtac tttatgttcg cgcagatgac gcgctaccag
    29341 ctcgatggtt tcgcccgcgc ctaccagcaa cactgtgacc gtagagagcg attcaaagat
    29401 ctgccgcgcc agcgtacaag ccgcaaaagc gacagacaca gcactggcac cgatatctgt
    29461 ttcagtgcga acgcgtttcg ctacagagaa agatttctgg aacatgcgtt ccagttcgct
    29521 ggccttcata tgaccttttt gcgaatcggc aaacgctttt ttaacctgac cgaggatctg
    29581 cggctcccca agaaccagtg aatccaggcc gctggcaaca cgcattaaat ggctaaccgc
    29641 gtcgttatcc tgatgccagt agaggctttt acgcagatct tcttcattaa gattgtgata
    29701 atcgcaaagc cagcggatta acgcctcttg caggttatcc tgctcttcaa cgctaagata
    29761 aagttccgtg cggttgcacg tcgacagcac cacgccgccc tgcaccatcg gctgcgcaag
    29821 caggctgtca agcgcctgat cgagcttatc cggcgaaaac gatacacgtt ctcgcagcga
    29881 tacaggtgcc gttttatggt tgataccgag tgctaaaagg gtcatgtctg cgggaaataa
    29941 taccaacgtt gatatggtta gtctgcttgc atcatacagg atgcgtaaga tcaataaaag
    30001 agagcgcccc cttttggagt aattgccgaa agccgttaga ttctggcaat taagacaact
    30061 tgaacataga cgatagcgga cggtaacgct agcattaagg gttataactg caacgtatct
    30121 caaggacttg tcatcactat gccccagccc gattttcgtc ttatccgcct gctaccgctg
    30181 gctgctcttg tgctcactgc ctgttccgtt accacgccca aaggtcctgg caaaagcccg
    30241 gattcgccac aatggcgtca gcatcagcaa gacgtgcgca atcttaatca gtatcagact
    30301 cgcggcgcgt tcgcttatat ttctgaccaa caaaaagtgt acgcccgctt tttctggcag
    30361 caaaccggcc aggatcgcta ccgtctgctg ctcactaacc cattgggcag cacggaactg
    30421 gagctgaatg ctcaaccggg taacgtgcag ttagtcgaca ataaaggtca gcgttatacc
    30481 gccgatgacg ccgaagagat gattggcaaa ttgaccggaa tgccaattcc gctcaacagc
    30541 ttgcgccaat ggattttagg tttaccgggt gatgcaaccg actacaaact ggacgaccag
    30601 taccgcctga gcgaaattac ctacagccag aatggcaaaa actggaaggt tgtttatggt
    30661 ggttatgaca ccaaaacgca acctgcgatg ccagccaata tggaactcac cgacggtggt
    30721 caacgcatca agttaaaaat ggataactgg atagtgaaat aatgcggaca cagtggccct
    30781 ctccggcaaa acttaatctg tttttataca ttaccggtca gcgtgcggat ggttaccaca
    30841 cgctgcaaac gctgtttcag tttcttgatt acggcgacac catcagcatt gagcttcgtg
    30901 acgatgggga tattcgtctg ttaacgcccg ttgaaggcgt ggaacatgaa gataacctga
    30961 tcgttcgcgc agcgcggttg ttgatgaaaa ctgcggcaga cagcgggcgt cttccgacgg
    31021 gaagcggtgc gaatatcagc attgacaagc gtttgccgat gggcggcggt ctcggcggtg
    31081 gttcatccaa tgccgcgacg gtcctggtgg cattaaatca tctctggcaa tgcgggctaa
    31141 gcatggatga gctggcggaa atggggctga cgctgggcgc agatgttcct gtctttgttc
    31201 gggggcatgc cgcgtttgcc gaaggcgttg gtgaaatact aacgccggtg gatccgccag
    31261 agaagtggta tctggtggcg caccctggtg taagtattcc gacaccagtg atttttaaag
    31321 atcctgaact cccgcgcaat acgccaaaaa ggtcaataga aacgttgcta aaatgtgaat
    31381 tcagcaatga ttgcgaggtt atcgcaagaa aacgttttcg cgaggttgat gcggtgcttt
    31441 cctggctgtt agaatacgcc ccgtcgcgcc tgactgggac aggggcctgt gtctttgctg
    31501 aatttgatac agagtctgaa gcccgccagg tgctagagca agccccggaa tggctcaatg
    31561 gctttgtggc gaaaggtgtt aatctttccc cattgcacag agccatgctt taagccgggc
    31621 aagctgagtt tcggtgacaa cgtcaccttg ttccagacgt tgcatcgcgc tctttaatac
    31681 accgcctgga aaggatcatg cctggcccgc acagttttcg gcagattctt tccaccaatg
    31741 gacgcatgcc tgaggttctt ctcgtgcctg atatgaagct ttttgctggt aacgccaccc
    31801 cggaactagc acaacgtatt gccaaccgcc tgtacacttc actcggcgac gccgctgtag
    31861 gtcgctttag cgacggcgaa gtcagtgtac aaatcaacga aaatgtacgc ggtggtgata
    31921 ttttcatcat ccagtccact tgtgccccta ctaacgacaa cctgatggaa ttagtcgtta
    31981 tggttgatgc cctgcgtcgt gcttccgcag gtcgtatcac cgctgttatc ccctactttg
    32041 gctatgcgcg ccaggaccgt cgcgtccgtt ccgctcgtgt accaatcact gcgaaagtgg
    32101 ttgcagactt cctctccagc gtcggtgttg accgtgtgct gacagtggat ctgcacgctg
    32161 aacagattca gggtttcttc gacgttccgg ttgataacgt atttggtagc ccgatcctgc
    32221 tggaagacat gctgcagctg aatctggata acccaattgt ggtttctccg gacatcggcg
    32281 gcgttgtgcg tgcccgcgct atcgctaagc tgctgaacga taccgatatg gcaatcatcg
    32341 acaaacgtcg tccgcgtgcg aacgtttccc aggtgatgca tatcatcggt gacgttgcag
    32401 gtcgtgactg cgtactggtc gatgatatga tcgacactgg cggtacgctg tgtaaagctg
    32461 ctgaagctct gaaagaacgt ggtgctaaac gtgtatttgc gtacgcgact cacccgatct
    32521 tctctggcaa cgcggcgaac aacctgcgta actctgtaat tgatgaagtc gttgtctgcg
    32581 ataccattcc gctgagcgat gaaatcaaat cactgccgaa cgtgcgtact ctgaccctgt
    32641 caggtatgct ggccgaagcg attcgtcgta tcagcaacga agaatcgatc tctgccatgt
    32701 tcgaacacta atcgaacccg ctcaaagacc cgctgcggcg ggtttttttg tctgtaatat
    32761 ccatttgtat gacctatgcc tccttcacct gccatttagt tgacagatga tgcgctcacg
    32821 gatgaaacat tattgtgaac aaaatatttt cctcacatgt gatgcctttc cgcgctctga
    32881 tcgacgcttg ctggaaagaa aaatatactg ccgcacggtt tacccgtgac ctgattgccg
    32941 ggataaccgt cgggattatt gctatcccgc tggcgatggc gttggctatt ggtagtggtg
    33001 tggcacccca gtacggttta tataccgcag ctgttgcggg gattgtcatt gctctgacgg
    33061 gtgggtcacg ctttagcgtt tccggtccga ctgcggcatt tgtggtaatt ctctatcccg
    33121 tgtcgcaaca gtttggactg gcaggactgc tggttgcgac cttgctgtcg gggatctttt
    33181 tgattctgat gggtctggca cgctttggtc gcctgattga gtatattccg gtttccgtca
    33241 ccttaggttt cacctcgggt atcgggatca ccatcggtac catgcagatt aaagattttc
    33301 tcggtctgca aatggcccat gtcccggaac attatctaca aaaagtcggc gcattattta
    33361 tggcgctgcc gaccattaat gtgggtgatg ctgccattgg cattgtgacg ctaggtattc
    33421 ttgttttttg gccgcgtctg ggcattcgtt tacccggtca ccttccggcc ttgctggctg
    33481 gttgcgcggt gatggggatt gttaacctgc tcggcggaca tgttgctacc atcggttcgc
    33541 aattccacta cgtcctggcc gatggttctc agggtaacgg tattccgcaa ctgctgccgc
    33601 aactggtgct gccgtgggat ctgcctaatt cagaattcac gctaacctgg gattctattc
    33661 gcacactgct gcctgcggca ttctcaatgg caatgctcgg cgcaatcgaa tctctgctct
    33721 gcgccgtggt actggatggt atgaccggga cgaaacacaa ggcgaacagc gaactggttg
    33781 gacagggact ggggaatatt atcgctccgt tctttggtgg tattaccgct acagctgcca
    33841 tcgcgcgttc tgccgctaac gtccgtgccg gggcaacttc ccctatctcg gcggtgatcc
    33901 actctattct ggttattctt gccctgctgg tactggcacc gctgctctcc tggctgccgc
    33961 tttccgctat ggcagccctg ctgttgatgg tggcgtggaa catgagtgaa gcgcataaag
    34021 tggtcgactt gctgcgtcat gcaccgaaag atgacatcat tgtcatgctg ctgtgcatgt
    34081 cgctgaccgt gctgtttgat atggttattg ccatcagcgt ggggatcgtg ctggcatcgc
    34141 tgctgtttat gcgtcgtatc gcacgtatga ctcgcctggc accggtagtc gtagatgttc
    34201 cagacgatgt tctggtactg cgcgttattg gcccgctgtt ttttgctgct gctgaaggct
    34261 tgttcacgga cctggagtca cgtcttgaag gcaaacggat tgtgattctg aagtgggatg
    34321 ccgttccggt acttgatgct ggtggtcttg atgcgttcca gcgttttgtg aagcgtctgc
    34381 ccgaaggatg tgaactgcgc gtgtgcaacg tggaattcca gccactgcgc actatggctc
    34441 gcgcaggcat tcaaccgatc ccgggacgcc tcgcgttctt cccgaatcgt cgcgcggcga
    34501 tggcggattt ataagtacga gattgaccag tcagcgcaaa ctgactggtc agcaaactgc
    34561 attatttgtt agctatgacg gcgattatcg ctacggcggc aacgtttgtc atagtgacga
    34621 acccaccagt aacggtcgca cacctgttca tgcccgccaa cacgggcacc cgccagccag
    34681 agaatggcac cgacgaaaat acttagcact gcaccatgtg cgaaatactg gggcatatta
    34741 aactgtggta actggttgag gattgaatac cccacgccga ccaccattac caccagcccc
    34801 aaccccatga gcacgttacc gagtaacgaa gcgtttttgc gtttcatatg tcacctccgg
    34861 aactttctgg gttgtaacag ggaatacccc tcttccttat gtgtaaagta tagacaacac
    34921 cctgtgggtt atgtgcgggc gtgatcacaa ttacaaccct tatttcaaca aaactttaca
    34981 aataaacgcc tgaacacctt gactttctaa tagtccactc aggcaattat tacgttatct
    35041 gcatttgata cgcagttttt tgtcaagcgg ggccgcaacc agtaaactac gcgccagtta
    35101 tgtacacact caggacaaaa aacgtgacga ttaaattgat tgtcggcctg gcgaaccccg
    35161 gtgctgaata cgccgcaacg cgacataatg ctggtgcctg gttcgttgac ttactggcag
    35221 agcgtttgcg cgctccgctg cgcgaagagg ctaaattctt tggttatact tcgcgagtca
    35281 ctcttggagg cgaagatgtc cgcctgttag tcccgactac atttatgaat ctcagcggca
    35341 aagccgttgc ggcgatggcc agttttttcc gcattaatcc ggacgaaatt ctggtggccc
    35401 acgacgaact ggatctgcct cctggcgtcg ccaaatttaa attgggcggt ggccatggtg
    35461 gtcacaatgg actgaaagac atcatcagta aattgggtaa taaccctaac tttcaccgtt
    35521 tacgcatcgg aatcggtcat cctggcgata aaaataaagt tgtcggtttt gtgttaggca
    35581 aaccgcctgt tagtgaacag aagttaattg atgaagccat cgacgaagcg gcgcgttgta
    35641 ctgaaatgtg gtttacagat ggcttgacca aagcaacgaa ccgattgcac gcctttaaag
    35701 cgcaataagt cgttgtctgc ggcatttttg ccgagtgccg tgtataatag gcaaagttat
    35761 ttccatttct gcaatctgtt agcaataaca ggttgattat taagatatta aggtgattta
    35821 aatcatggga ttcaaatgcg gtatcgtcgg tttgcccaac gtcgggaaat ctaccctgtt
    35881 caacgcgctg accaaagccg gtattgaagc ggccaacttt ccattctgca ccattgagcc
    35941 gaacacaggc gtcgtaccaa tgcctgaccc tcgcctggat caactggctg aaatcgtaaa
    36001 accgcaacgt acgcttccca cgaccatgga atttgtcgat atcgccggtc tggtaaaagg
    36061 cgcatcgaaa ggcgaaggtc tgggtaacca gttcctgacc aacatccgtg aaaccgaagc
    36121 gatcggtcac gttgttcgct gctttgaaaa tgacaacatc attcacgttt ccggcaaagt
    36181 taacccggct gacgatattg aagttatcaa caccgaactg gcgctggcag acctcgacac
    36241 ctgcgaacgt gcgattcatc gcgtacagaa gaaagccaaa ggtggcgata aagacgcgaa
    36301 agctgagctg gcggtcctag aaaaatgcct gccccagctg gaaaacgcag gtatgctgcg
    36361 cgcgctggat ttaagcgctg aagagaaagc ggctattcgt tacctgagct tcctgacgct
    36421 gaaaccaaca atgtacatcg ccaacgtcaa cgaagacggt tttgaaaaca acccatatct
    36481 tgaccaggtg cgtgaaatcg cggcgaaaga aggttctgtt gtggttccgg tttgtgctgc
    36541 tgttgaagca gacattgccg aactggacga cgaagaacgt gacgagttta tgcaggagct
    36601 tgggctggaa gaaccgggcc tgaaccgtgt gatccgtgtc ggttataagc tgctgaacct
    36661 gcaaacttac ttcaccgctg gggtgaaaga agtgcgtgca tggaccattc cggttggagc
    36721 aaccgcgccg caggcagcgg gcaaaatcca tactgatttt gaaaaaggct ttatccgtgc
    36781 acaaaccatc tcgtttgaag atttcatcac ttacaaaggt gaacaaggcg cgaaagaagc
    36841 aggcaaaatg cgtgcagaag gtaaagatta catcgttaaa gatggcgatg tgatgaactt
    36901 ccttttcaac gtctaattaa tttattgtct catgaggttt cgctaaatct catgaagagc
    36961 gcaaaagtat ataaaatcca cgcaattgcg tggatttttc atttcaccga gtcctaatca
    37021 ttcttgtaac atcctacact tttgtgctta ttgcctacag tgaaccacga gaataattaa
    37081 agacatcagt gtcagatgac ggatatctca ttagtagtga tataacgtaa gtttttgttc
    37141 aactacacat cacccccttc acagctgatc aatattgatc acgctccaat atgctggcga
    37201 gaaaaggact tacctgaagg gtaatatccc gcagataaag tccccgaata acgtgattac
    37261 gtgcgatcct ggcaagatga aaatgataac cagtagcaca ataacaacga taggccagaa
    37321 aacccattga ccaaaaatga atatcgatgt tttttgcttt ttcttattag taatgcaaaa
    37381 atggcgcata ttgtttttgt tatgcagata catcaagcga aattcccata actaattagg
    37441 attattacaa aaaatcttca ccaaattttt taaccgctgt ttgttgtaat atttttgcca
    37501 tatttatagt aacgaattgg aataagtaac gacaacttag ggaataactc gatataaact
    37561 cacctttgta agggggatgt taaatatata ttaattcctg tgttcctgat tttgcccggt
    37621 aatagagaat tattctctga gaagctcatc atgattgcac catacaaata tcatgatgat
    37681 aagaggatat tattcattga tttaaacaaa atgacaaaat ccatagaccc atagtctggg
    37741 tactatatag gagtggtatg ggcatcaaac aacacaatgg gaataccaaa gcagatcgtc
    37801 tcgctgaat
//
LOCUS       DADMKF010000023        37257 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.023, whole genome shotgun sequence.
ACCESSION   DADMKF010000023 DADMKF010000000
VERSION     DADMKF010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 37257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37257
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.023"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(<1..642)
                     /locus_tag="JNC24_001061"
     CDS             complement(<1..642)
                     /locus_tag="JNC24_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase YliE domain
                     protein"
                     /protein_id="HAY9157872.1"
                     /translation="MVKKPQQGTIFAAAQRSDSYFVKCINMLSLYEKIKIRLIILFLL
                     AALSFIGLFFIINYQLVSERAVKRADSRFELIQKNVGYFFKDIERSALTLKDSLYLLK
                     NTEEIQRAVILKMEMMPFLDSVGLVLDDNKYYLFSRRANDKIVVYHQEQVNGPLVDES
                     GRVIFADFNPSKRPWSVASDDSNNSWNPAYNCFDRPGKKCISFTLRINGKDHDL"
     gene            complement(742..1653)
                     /gene="gsiD"
                     /locus_tag="JNC24_001062"
     CDS             complement(742..1653)
                     /gene="gsiD"
                     /locus_tag="JNC24_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001236035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HAY9157873.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGAAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(1656..2576)
                     /gene="gsiC"
                     /locus_tag="JNC24_001063"
     CDS             complement(1656..2576)
                     /gene="gsiC"
                     /locus_tag="JNC24_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HAY9157874.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGLEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMLLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(2594..4132)
                     /gene="gsiB"
                     /locus_tag="JNC24_001064"
     CDS             complement(2594..4132)
                     /gene="gsiB"
                     /locus_tag="JNC24_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HAY9157875.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYTVKLREGIKFQDGT
                     DFNAVAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPAALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQATLLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(4152..6023)
                     /gene="gsiA"
                     /locus_tag="JNC24_001065"
     CDS             complement(4152..6023)
                     /gene="gsiA"
                     /locus_tag="JNC24_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HAY9157876.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLQRRSREVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYPHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQAPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKDAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGPRRAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(6010..6975)
                     /gene="iaaA"
                     /locus_tag="JNC24_001066"
     CDS             complement(6010..6975)
                     /gene="iaaA"
                     /locus_tag="JNC24_001066"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HAY9157877.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFARGMERVSPEIFSTPLRYEQLLAARKEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCIGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            7179..8414
                     /gene="moeA"
                     /locus_tag="JNC24_001067"
     CDS             7179..8414
                     /gene="moeA"
                     /locus_tag="JNC24_001067"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HAY9157878.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTAQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VDVGEWVEVEPFNALFGGL"
     gene            8414..9163
                     /gene="moeB"
                     /locus_tag="JNC24_001068"
     CDS             8414..9163
                     /gene="moeB"
                     /locus_tag="JNC24_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HAY9157879.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLIVGLGGLGC
                     AASLYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAALIAEHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     TSGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(9342..10004)
                     /gene="fsa"
                     /locus_tag="JNC24_001069"
     CDS             complement(9342..10004)
                     /gene="fsa"
                     /locus_tag="JNC24_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HAY9157880.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDARKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVEAAVAKFEQDWQGAFGRT
                     SI"
     gene            10135..11034
                     /locus_tag="JNC24_001070"
     CDS             10135..11034
                     /locus_tag="JNC24_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HAY9157881.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMTTVLRDKPFYDRSGGGLTLSGGEPFMQPEMAMALLQASHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            11040..13472
                     /locus_tag="JNC24_001071"
     CDS             11040..13472
                     /locus_tag="JNC24_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HAY9157882.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALAREMAATETRESRRDELLAIAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIELLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQTLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            13618..14433
                     /gene="ybiV"
                     /locus_tag="JNC24_001072"
     CDS             13618..14433
                     /gene="ybiV"
                     /locus_tag="JNC24_001072"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HAY9157883.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIKFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKAVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            14585..15850
                     /locus_tag="JNC24_001073"
     CDS             14585..15850
                     /locus_tag="JNC24_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003570920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HAY9157884.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLDRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRRPPGTTSKGLGAHTDSGALERWLLPAYQ
                     RVFANVFNGNLAQYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSNMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGIDS"
     gene            complement(15817..16920)
                     /locus_tag="JNC24_001074"
     CDS             complement(15817..16920)
                     /locus_tag="JNC24_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAY9157885.1"
                     /translation="MDKKLKIADIAARTGLSPSTVSRVLAGKANTSARAREQVLACAR
                     ELGVMDGMAAGRMLLNNLIIFAPQRAFDQRSDIFYYRVIQSISKALSPHEVRLRYCAL
                     EEFDSDPALFLSRMNEADTQAAILLGIDDPHIHDLAADLAKPCVLINCRDERMRLPAI
                     APDHRLIGAFAARYLFEMGHREVMNVMCLRRYTMEQRFAGIKESWRQHNLAFHHDRHL
                     LNISSFSAKEAEEKVGAWLDAANGKTLPTAFLVGGDFMAAGTVNALHRRGLRVPQDVS
                     VMSIDGFNLAAIEDVPLTAVHVPRDELGNEAVQMLQQRLIRPDAPVGSLLLHGRLAVR
                     ESVRRVRPGKGNTAVEREGLYDTQLSIPNARLP"
     gene            17215..18516
                     /locus_tag="JNC24_001075"
     CDS             17215..18516
                     /locus_tag="JNC24_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY9157886.1"
                     /translation="MSQGINNDMTASKPRHIVKNLRWWILVLFLLGVTVNYITRNSLG
                     ILAPELKSSLGITTEQYSWIVGAFQLAYTLFQPLCGWLIDVIGLKLGFMICAGLWALA
                     CLMHAGAGNWGHLALLRFFMGGAEAAATPANAKTIGEWFPKSERPIAAGWAGVGFSIG
                     AMLAPPIIYFAHASFGWQGAFMFTGILAMLWVVLWWAFYHSPDKHPNLSKAELDFIRQ
                     DNEAPPVKLPFLTALKTVGKNKRFYGIAIPAFMAEPAWAVMSFWVPLYLSKTYGMDLK
                     QIALFAWLPFLAADLGSIASGYLTKLYTRLFGLSRVNSVVASSVSGAFLMVSLAVMAF
                     TNNPYIAIVLISIGGFGHQIISCMLSALVVESFDKGQMATVNGMRGSAAWIASFLFSL
                     IIGVTADKIGFNPLFIAMGFFDLIGAFFLVTFIAERRAKRA"
     gene            18531..20894
                     /locus_tag="JNC24_001076"
     CDS             18531..20894
                     /locus_tag="JNC24_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HAY9157887.1"
                     /translation="MKTLKNWTLHKQSAHHVELLVDGQHILCLYVLEENLFRVLLKRK
                     GELALDRTWSIAPAQDVPWEGRSREDLSGFSLPAWTVIQHDDTVVIATESLRATVHQP
                     LWLEWHYRNNEGEWLPLVNDRPTSAYLLNAHGDGVAHYQSRRKDERFYGLGDKAGDLQ
                     RNGKRYEMRNLDAMGYNAVSTDPLYKHIPFTITRRDDISFGLFYDNLSSCWLDLGNEI
                     DNYHTAYRRWQAEAGDIDYYLFTGERVLDITKAFVRLTGKTLFGPKWSLGYSGSTMHY
                     TDAPDAQNQLMNFIRLCEEHAIPCDSFQLSSGYTSINGKRYVFNWNYDKVPQPKVMSQ
                     AFHNAGLHLAANIKPCLLQDHPRYNEVAERGLFIRDSETDEPERSSFWDDEGSNLEFT
                     NPQTIQWWQEGVTTQLLEMGIDSTWNDNNEFEVWDGEARCHGFGQTIAIKHIRPVMPL
                     LMMRASMEAQQRFAPEKRPYLISRSGCAGMQRYVQTWSGDNRTSWDTLRYNTRMGLGM
                     SLSGLYNLGHDVGGFSGDKPDPELFVRWVQNGVMHPRFTIHSWNDDHTVNEPWMYPGV
                     TPAIRSAIELRYRLLPYFYTLMWQAYADDEPMLRPTFLDHEHDVQTFEECDDFLLGRD
                     ILVASVVEQGERQRRVWLPDNETGWYDFYNGEWFSGGQWITLDAPLEKLPLMVRAGAG
                     LPLSKRITYVSAEQDDTRELKLFPLKGVGTTSGLLFEDDGESWGYQTGNALWVEWEMV
                     CDGATINLKVNARGDYRPAWSALKVSLPLEEKRTLLVNGVEGSEWMR"
     gene            complement(21074..22669)
                     /locus_tag="JNC24_001077"
     CDS             complement(21074..22669)
                     /locus_tag="JNC24_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HAY9157888.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFADPEILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERAIDFSGNYEDYLRSKGIDA"
     gene            22888..23808
                     /gene="ldtB"
                     /locus_tag="JNC24_001078"
     CDS             22888..23808
                     /gene="ldtB"
                     /locus_tag="JNC24_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HAY9157889.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVERKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(23867..24985)
                     /locus_tag="JNC24_001079"
     CDS             complement(23867..24985)
                     /locus_tag="JNC24_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HAY9157890.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWSCLGLYIVFLTALE
                     FKQELWGLVIVAAGFALLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAIGLSQVISNVPSTILLLNYVPPSLLLVWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVILPAN"
     gene            complement(24982..25449)
                     /locus_tag="JNC24_001080"
     CDS             complement(24982..25449)
                     /locus_tag="JNC24_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MntR"
                     /protein_id="HAY9157891.1"
                     /translation="MSRRAGTPTAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            25635..25763
                     /gene="mntS"
                     /locus_tag="JNC24_001081"
     CDS             25635..25763
                     /gene="mntS"
                     /locus_tag="JNC24_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HAY9157892.1"
                     /translation="MNEFKKCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            26035..27618
                     /locus_tag="JNC24_001082"
     CDS             26035..27618
                     /locus_tag="JNC24_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HAY9157893.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSDDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(27667..28182)
                     /gene="ompX"
                     /locus_tag="JNC24_001083"
     CDS             complement(27667..28182)
                     /gene="ompX"
                     /locus_tag="JNC24_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HAY9157894.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(28235..28300)
                     /locus_tag="JNC24_001084"
     CDS             complement(28235..28300)
                     /locus_tag="JNC24_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9157895.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            28535..29422
                     /gene="rhtA"
                     /locus_tag="JNC24_001085"
     CDS             28535..29422
                     /gene="rhtA"
                     /locus_tag="JNC24_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HAY9157896.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
     gene            29721..30224
                     /gene="dps"
                     /locus_tag="JNC24_001086"
                     /gene_synonym="pexB"
     CDS             29721..30224
                     /gene="dps"
                     /locus_tag="JNC24_001086"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HAY9157897.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            30628..31374
                     /gene="glnH"
                     /locus_tag="JNC24_001087"
     CDS             30628..31374
                     /gene="glnH"
                     /locus_tag="JNC24_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HAY9157898.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFGTEPK"
     gene            31513..32172
                     /gene="glnP"
                     /locus_tag="JNC24_001088"
     CDS             31513..32172
                     /gene="glnP"
                     /locus_tag="JNC24_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HAY9157899.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            32169..32891
                     /gene="glnQ"
                     /locus_tag="JNC24_001089"
     CDS             32169..32891
                     /gene="glnQ"
                     /locus_tag="JNC24_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017491583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HAY9157900.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALENVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     DPQVLIKNPPSQRLQEFLQHVS"
     gene            33008..35233
                     /gene="ybiO"
                     /locus_tag="JNC24_001090"
     CDS             33008..35233
                     /gene="ybiO"
                     /locus_tag="JNC24_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
                     /protein_id="HAY9157901.1"
                     /translation="MRWILFILFCLLGAPAHAVSIPGVTTTTTTDSTTEPAPEPDIEQ
                     KKAAYGALADVLDNDTSRKELIDQLRTVAATPPAEPVPKIVPPTLVEEQTVLQKVTEV
                     SRHYGEALSARFGQLYRNITGSPHKPFNPQTFSNALSHFSMLAVLVFGFYWLIRLCAL
                     PLYRKMGQWARQKNRERSNWLQLPAMIIGAFIIDLLLLALTLFVGQVLSDNLNAGSRT
                     IAFQQSLFLNAFALIEFFKAVLRLIFCPNVAELRPFTIQDESARYWSRRLSWLSSLIG
                     YGLIVAVPIISNQVNVQIGALANVIIMLCMTVWALYLIFRNKKEITQHLLNFAEHSLA
                     FFSLFIRAFALVWHWLASAYFIVLFFFSLFDPGNSLKFMMGATVRSLAIIGIAAFVSG
                     MFSRWLAKTITLSPHTQRNYPELQKRLNGWLSAALKTARILTVCVAVMLLLSAWGLFD
                     FWNWLQNGAGQKTVDILIRIALILFFSAVGWTVLASLIENRLASDIHGRPLPSARTRT
                     LLTLFRNALAVIISTITIMIVLSEIGVNIAPLLAGAGALGLAISFGSQTLVKDIITGV
                     FIQFENGMNTGDLVTIGPLTGTVERMSIRSVGVRQDTGAYHIIPWSSITTFANFVRGI
                     GSVVANYDVDRHEDADKANQALKDAVAELMENEEIRGLIIGEPNFAGIVGLSNTAFTL
                     RVSFTTLPLKQWTVRFALDSQVKKHFDLAGVRAPVQTYQVLPTPGATPAEPLPPGEPT
                     L"
     gene            complement(35230..36156)
                     /gene="rlmF"
                     /locus_tag="JNC24_001091"
     CDS             complement(35230..36156)
                     /gene="rlmF"
                     /locus_tag="JNC24_001091"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HAY9157902.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVAFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            36432..36692
                     /locus_tag="JNC24_001092"
     CDS             36432..36692
                     /locus_tag="JNC24_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HAY9157903.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMFGTATIYK"
     gene            36957..>37257
                     /locus_tag="JNC24_001093"
     CDS             36957..>37257
                     /locus_tag="JNC24_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HAY9157904.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQTNADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNAPG"
BASE COUNT         9032 a        10059 c         9339 g         8827 t
ORIGIN      
        1 caaatcgtga tctttgccgt tgatacgtag cgtaaaagag atacattttt tacccggacg
       61 atcaaagcaa ttgtatgccg gattccagct gttgttagag tcatctgaag ccaccgacca
      121 cggtcgtttc gatgggttaa aatcggcaaa aataacccgc cctgactcgt cgacaagcgg
      181 tccatttact tgttcctgat gataaacaac gattttatca ttcgccctcc gcgaaaagag
      241 ataatattta ttatcatcaa gtaccagtcc caccgagtct aaaaatggca tcatttccat
      301 tttcagaatc acggcgcgtt gaatctcctc tgtatttttt aataaataca gtgagtcctt
      361 taatgtcagg gccgaacgtt caatatcttt aaagaaatag ccaacgtttt tctgaataag
      421 ttcaaagcgg ctatcggcac gttttaccgc gcgttccgat accagttgat agttaatgat
      481 gaaaaaaaga ccaataaatg acagtgctgc caataaaaat aaaattatca gccttatctt
      541 tatcttttcg tataaactca gcatgttaat acatttaaca aaataactat ctgaacgctg
      601 tgccgctgca aaaatagtcc cttgctgcgg ctttttaacc aaattacccg attctacaaa
      661 atcagcagaa agttacaaca cttgcttagg gcaataattt cctgtgaggt gattaccctt
      721 tcaagcaata ttcaaacgta actatccttt aattttcgga tccagcgcat cgcgtaaacc
      781 atcgcccaac aaattgaacg ccagtacggt cagaaaaata gccagggccg gaaaaacagc
      841 gacatgcggc gcgataacca tatccgctcg tgcctcattg agcattgctc cccactctgg
      901 tgtcggcggc tgcgcaccga ggccgagaaa tgacaggctg gcggcagaga taatcgaggt
      961 gccaatgcgc atggtgaaaa acaccacgat agacgagacg gtcccaggca agatatgacg
     1021 caataaaatg gtcatatcgc tggcaccaat actgcgtgct gactcaataa aggtttgctg
     1081 tttcaacacc agcgtgttgc cgcgcaccag tcgggcaaac gcggggatgg aaaaaatggc
     1141 gactgcgata atcacgttag caatgccgct tcccaacacc gcaacaacag cgatcgccag
     1201 taaaataccc gggaaggcaa acagcacatc gcaaatgcgc atgatcagcc gatcccacca
     1261 gccttcataa tatccagcca acaagcccag caacgtcccg atcgccgcac cgataaacac
     1321 ggcaaacacg cccgccgcca gcgagatttg cgcaccaacc aggacacggc tgaaaatgtc
     1381 gcgccccagt gaatcgacgc caaaccagtg ctgcaaagaa ggtccgttat tcagattgtc
     1441 ataatcaaaa taattttcgg cgtcataggg agcgatccag cgtgcaaaaa tggccaccac
     1501 aatcaataaa ataacgaata atgcggcggt catcgccata tgctgacggc gaaatcgtcg
     1561 ccagaattca tgccacggtg tacgcacctg gtcaggtttg accagtggca tggcgtttaa
     1621 caccgcctga cgtcgccagt taaatagtcg catccttact tgtacctgat agccgggtta
     1681 atggcggcgt aaagcacatc caccactaag ttgataagaa taaattccag cgagaaaagc
     1741 agaatttccg cctgaatcac cgggtaatcg cgcatttcta cggagtcaac gagtaagcgt
     1801 ccaagtccgg gccagttgaa aactttctcc accacgatag aaccaccgag caaaaagcca
     1861 aactgtaagc ccatcatggt cactaccggg atcatcgcgt tacgtagccc gtgtttgagg
     1921 acaacccagg tttcgctcac ccctttcgcc ctcgcggtac gcatataatc ttcgcttaaa
     1981 acatcgacaa acgacgcgcg ggtaaagcgc gccatcacgg ctgccactgc cgcgccgagc
     2041 gtcagggagg gtaaaatgta gtgctgccaa ctgtctgctc ccacggtagg cagccagccc
     2101 agttcaacgg agaatacctg aattaaaagc atccccagcg caaatgccgg aaacgagatc
     2161 cccgacaccg caatggtcat actcaatcga tccggccaac gattacgcca gacggcggcg
     2221 ataattcccg ccgccatacc aaatatcacc gcccagacca tactggttat ggtcagccac
     2281 agcgttggca taaagcggct ggcaatctca tcggcaaccg gacgacgcga caccatcgac
     2341 aggccaaaat ccccctgcac agcattgctg atatagtgcc agaactggtg atacagcggc
     2401 tgatccaacc ccagctgctg acgcaccagt tctataacct gcgcatcagc ttcgagcccg
     2461 gcaatcaatc gcgccggatc gccgggcagc atatgcacaa ataaaaacac cagcaccgag
     2521 acgataaaca gcgtcggaat caaccccagt aagcgtttga taacgtaatt aagcattcca
     2581 ctccctgcgt tgcttattgc aaatccgcgt cttcaaagct gaagccggtg tctggcatga
     2641 tccaaaaacc ggtcaggttt ttactgtgtg ccgacaccag tttttctacc accagcggga
     2701 tccacggcga ctcttgccag atgatatcct gcgccgcctt atacaagcgg gtcttttccg
     2761 ccggatcatt agttttcagt gcctgagcca ggaagtcgtc cacctgttta ttgctgtaaa
     2821 atgcggtatt aaacagcgtc ggtggccagt tttgcgaggc gaacagtggc gatagcgccc
     2881 agtccgcttc gccggttgaa gccgaccagc cagtgtagaa catccgcacg ccgctctctt
     2941 tttgcccttt accttcaact tctgccgccc gctgtccggc atccatcgcg gtcacctggg
     3001 ctttaatccc gacctgcgct aactgctgct gggtaaattg cagcactttc tgcgcggtgc
     3061 tgtggttatg tgacgaccac agcgtggtac tgaaaccgtt gggatatccc gcctctttca
     3121 gtaattcgcg cgctttcact ggatcgtaag gccacggttt ataactttgc gcgtaggcga
     3181 tacttggcgg taccacacca gtagctggcg ttgcatagcc cgcaaaggca actttcacca
     3241 gcgccggacg gttaatggcg taattcagcg cctcacggac cttcgggtta tcgaacggct
     3301 tttgcgtcac gttcatactg atataacgct gcataattga cggactggcc atcaactcga
     3361 tatttttgtt tttctccagc agtgtggcct gctcgtaggg aatggggaaa gcaaactgcg
     3421 cttcaccggt ttgcagcatt gccgcgcggg tgttgttatc cgccaccgga cgccaggtta
     3481 tgctgtccag tttgggcaat cctggctgcc agtaacccgc gaattttttc accttcacaa
     3541 aatcggtctg attccaggta tccagttcat acggtccggt tcccaccgga tgaaaaccaa
     3601 tctccttgcc atatttttcc agcgctgccg gtgaaatcat cgcggtcgcc ggatgggcaa
     3661 gaatattaat aaacgctgag aacggctgtt tgagggtaat ctttaccgtt gtcggatcga
     3721 tcgcctccgt tttggcaata ttcttataca ggttatagcg tttaagatga ttcgccggat
     3781 cgctggcccg gtccagattc gcttttaccg ccactgcgtt gaaatcggtg ccatcctgga
     3841 atttaatgcc ttcccgcaat ttcacggtgt aagtaatgcc gtcatcggaa acggtataac
     3901 tctccgccag cacgtttttc agtttcatct ctttatccag accgaacagc ccctggtaaa
     3961 acgatttcgc tacggcctga gataacgtat cattcgcgtc atacggatcg agcgtggtga
     4021 aattcgatcc taccgccacc accacatctt tggcagcgaa tgcacaagat gccatcaacg
     4081 ctgtcgcaat gcccagcgcc actaacccac tacggtgtac agctcttgcc attttattct
     4141 ccgcctgaat gttatctacg catgaatgcg tattctgatt gtggttgtgc gacgtaatgc
     4201 cccggcccga cgcattgcaa cgagacggct gccacctctt cgccacgcag atgaatattg
     4261 ctgggaagat cgtccgacag cagtacacgc tgcggtcgtt gtcgggacgg ttcagcgacc
     4321 ggaactgccg ccagtaattt acgcgtataa ggatgctgcg ggttttcgaa gaccgcgcgc
     4381 cgtggaccaa tttcaacaat ttgcccgaga tacatcaccg ccacacgatg actaatccgc
     4441 tctaccacgg ccatatcgtg ggagataaac agatacgcaa tgccgaaatc acgctggaga
     4501 tcgagcaaca agttgataat ctgcccgcga atagaaacat ccagcgccga aacggcttcg
     4561 tcggcaatga tcacttttgg attcaatgcc aacgcgcgag caatgcagat gcgctggcgc
     4621 tgaccgccgg aaaactcatg cgggtaacgc caggcatgtt caggtaacag gcccacgcgc
     4681 tccagcaacc acgcaacgcg tgcagccgcg tctttacctg gcaataaacc gtgtacacgc
     4741 agcggttcga taatcgaatc accgatggtc tgacgtgggt ccagcgaagc gtaagggtcc
     4801 tgaaaaataa actgaatatc ccggcgcaat gcctgaagtt tgccgggtga caaggtatcg
     4861 attctctgac cgttaaagat aatttcgccg ccctgcgatt cgaccaggcg cagcaacgcc
     4921 cgcccggtag tggatttacc gctgccagac tcgcccacca gcgatagcgt ttcgccaggc
     4981 cagagatcaa aactgacttt ctcaacggca tgcacttccc gcgttacgcg attcaacaaa
     5041 ccgctgcgca aagggaaacg ggtgacaaga ttacgcactc gtaaaacagg ttcgccatcc
     5101 accaccgttt tctgctcgat ggggggggcc tgtttcgctg gatgttcaag cgatatcaac
     5161 gggaaacgtc ggggataatc caaccctttc atcgcaccaa gttgcggaac agcagctaac
     5221 agcgcacggg tgtaaggatg ttgcggtgca tgaaaaatct gttcgacggt acccgtttcc
     5281 accgcctcgc cctgatacat caccagtacc cgatcggcaa tctctgccac cacgcccata
     5341 tcgtgagtga taaagataac gcccatcgac atctcttttt gcaatacttt gattaattgc
     5401 aggatctgcg cctgaatagt gacatccagc gcggtggttg gctcatcggc aatcagcacc
     5461 gccgggcggc atgacagcgc catcgcaatc atcactcgct ggcgcatccc gccagagagt
     5521 tgatgcggat aacgtgaaag aatggtttgt gcctcaggaa tgcgtacctg atccagcatc
     5581 cgcttcgcct cgaccatcgc ttcttcacga ctggcgttct gatgcagacg aattgattcg
     5641 gcaatctgtt cacccacagt aaataccggg ttcagcgatg tcatcggctc ctgaaatatc
     5701 atcgccatat ccgcaccgcg aacatggcgc atttgtgcag cgctctgctc gctaagttca
     5761 atcacttcgc gactgcgccg ctgcaacagc attttatcgc actgtactaa accgcccgcc
     5821 tgttccaaca ggcgcatcaa tgccaacgca gtcactgact taccggagcc ggattcgcca
     5881 acaattgcca gcgtctcacc gcgttgcaga ctaaaagaga gattgcggac cgcagctatt
     5941 ttctgctggt cctgcataaa ggcaatattc agattttcaa ccgccagcac attaccggca
     6001 tcaagttcat cactgtgtgg caacggtgtc ccctttttca cggtagatac cggtggttgg
     6061 cgtatcgcct gcgtagcccc aggcgcgata cattccttcg gtgttaaacg gtagcgcgac
     6121 attcccttca tggtcgatag cgattaagcc accgctaccg ccaagcgcag ggagtttttc
     6181 cattactacc cgctcgcagg cttccgcgag acttaatccg ccgtaatcca ttaacgcggc
     6241 gatgtcatat gccgccagcg cgcggatgaa gacttcgccc gtgccgatac aagaaaccgc
     6301 cacactggcg ttattggcgt agcatccggc acccactaag ggactatcgc caactcgtcc
     6361 gggtaattta ttggtcattc cgcctgtgga cgtggctgcc gccaaattgc cgtctaaatc
     6421 caacgccacg gcccccacgg tgcccatttt ctgtttttca tccagtggcg caccgctatg
     6481 gtcgaggacg gttgcccctt ccttgcgcgc tgccagtagt tgttcataac gcaaaggcgt
     6541 ggagaaaatc tccggcgaga cgcgctccat gccacgagca aacgcaaaat tttctgcccc
     6601 ttcgccaatc atcatcacat gcgggctttg ctccatcacc agccgggcgg caagaaccgg
     6661 attacgcaga tgactaacgc ccgccaccgc accggctttc agggtgttac catccatcac
     6721 acaggcgtcc agttcatggg tttcatcacg cgtaaagaca gcgccaattc cggcgttaaa
     6781 cagtggacac tcttccagca gacgcaccgc ttccgtcacc acatccagcg cactttcgcc
     6841 cgcttccagc attttctgcc cggtttcaac aatggcagac aacgcctcaa tgtagcgtaa
     6901 ttcctgttgc agactcatct gcgcgcggct aattgcacct gcgccaccat gaattgcaat
     6961 gactgctttg cccatagtat tcgtccatta agggtggctc gccacttttg ctataaatat
     7021 cagaattttt atgaattact cattcaattt ttgaatatag aaagatggat atggcatgta
     7081 aaggcaggag ccaacgctac gcccgaggtg gcatacagtc tgtggcgttt ttctgacata
     7141 ataggcaaat tcgattttgc ctccgcagga gtgttttcat ggaatttacc accggattga
     7201 tgtcgctcga caccgcgctt aatgagatgc tttctcgcgt caccccactg accgcccagg
     7261 aaacgctgcc actggtacag tgttttggtc gtattctggc gagcgatgtc gtttcgccgc
     7321 ttgatgttcc ggggtttgat aactccgcaa tggacggcta cgcggtgcgt ttagccgata
     7381 ttgcctccgg gcaaccgctg cccgttgccg gtaaatcctt tgccggtcag ccgtatcatg
     7441 gtgaatggcc tgcgggcacc tgcattcgta ttatgaccgg tgcgccggtg ccggaaggct
     7501 gcgaagcggt ggtgatgcag gagcagactg aacaaacgga caatggcgtg cgttttactg
     7561 ctgaagtgcg tagcgggcaa aatattcgcc gtcgcggtga agatatctct gcaggtgcgg
     7621 ttgttttccc ggcgggaact cgcctgacta ccgcagagct gccagtaatt gcttcactgg
     7681 ggattgccga agttccggtg attcgtaaag tgcgtgtagc gcttttttct accggtgatg
     7741 aactccagtt gcccggtcag ccgctgggcg acggccaaat ctacgacacc aaccgtctcg
     7801 ccgtacacct gatgttagaa cagctgggtt gcgaggtaat caacttaggg attatccgcg
     7861 acgatcccca tgccctgcgc gccgcattta ttgaagccga cagccaggcg gatgtagtga
     7921 tcagttccgg cggtgtttca gtgggtgaag cggattacac caaaacgatt cttgaagagc
     7981 tgggggagat cgccttctgg aagctggcga ttaaaccagg taaaccgttc gcgttcggta
     8041 aactcagcaa tagctggttc tgcggcctgc cgggcaaccc ggtttcagcg acgctgacct
     8101 tctatcaact ggtacagcct ttgctggcaa aactaagcgg caacaccgcc agcggcctgc
     8161 ccgcgcgcca gcgcgtacgc acagcgtccc gcctgaaaaa aacgccagga cgtcttgatt
     8221 tccagcgcgg cgtgctgcaa cgcaacgccg acggtgaact tgaagtgacg accaccggac
     8281 atcagggttc acatatattt agctccttta gtctcggcaa ctgctttatc gtgctggaac
     8341 gcgatcgcgg caatgtggac gtgggcgaat gggtggaagt agaaccgttt aacgcgttgt
     8401 tcggaggcct gtaatggcgg aactcagcga tcaggagatg ctgcgctaca accggcaaat
     8461 cattctgcgc ggttttgatt ttgacggtca ggaggcgcta aaagattctc gtgtgctgat
     8521 cgtcggcctc ggtggactcg gctgcgcagc ttcgctgtat ctggcaagcg ccggtgtcgg
     8581 caacctgacg ctgctcgact tcgacacggt ttcgctctcg aatctgcaac gccagacact
     8641 gcacagtgat gccacggtcg ggcaaccgaa ggtggaatcc gcccgtgacg ccctgacgcg
     8701 gatcaaccca catatcgcga ttacgccagt caatgcactg ctagatgacg cagaacttgc
     8761 agcattgatt gctgaacacg atctggtgct cgactgtacg gataacgttg cggtacgtaa
     8821 tcaactgaac gcaggctgtt ttgccgcgaa ggtgccgctg gtttccggcg cggcaattcg
     8881 tatggaaggg caaatcacgg tcttcactta tcaggacggc gaaccgtgct atcgctgcct
     8941 tagccgtttg tttggcgaga acgcattaac ctgcgtagaa gcaggcgtga tggcaccgtt
     9001 gatcggcgta attggttcgt tgcaggcaat ggaagcgatc aaactgctgg caggttatgg
     9061 caaaccgacc agcgggaaaa tcgtcatgta cgatgcgatg acctgtcagt ttcgcgaaat
     9121 gaaactgatg cgcaatccgg ggtgtgaggt gtgcgggcag taattgccac ctccaggcct
     9181 gataagacgc gacaagcgtc gcatcaggca tttgctccag acgccagatg cggcgtgaac
     9241 gccttatctg gcctacggtt cgggcacaga tttccaagcg cggcaggcat cggtgcactc
     9301 cgccacgtca tcagcaattt atgaggatgc agaacgtccg gttaaatcga cgttctgcca
     9361 aacgctccct gccagtcctg ctcaaacttc gccacagcgg cttccaccgc cggatagcta
     9421 atcatctgtt gtgccacatc cagtggcaga gtaattgatt cacatcctgc cagtaagcag
     9481 tccagcgcct gacgcggggt tttgaaactc gctgccagca ctttcgcctg cggcgcatgc
     9541 attttcaata actggtgtaa gtcggtcaca gtctgaatgc cgctaccgcc ctgagcatca
     9601 atacgattaa cgtaaggcgc aacatattcc gcacctgcca gcgccgacag caacccttgt
     9661 gctgcgccat ataccgcggt tcccagcgtt ggaatccctt ccgcttttaa catcttaata
     9721 gctgccagcc cctcggcggt caccggaact ttcaccacga tatccgcaat aatagaacgc
     9781 aatttacgtg cgtcattcac catcccttca gcagtggtag ccattacctg ggcaaacaga
     9841 cgcccctgac cgcccatcgc ttcatgaagt tgcggaagca caacatccag cggtttttta
     9901 cccgcggcga taatgcttgg gttagtggtc acacccgcca gcggaaaaat acgtgacagc
     9961 gccttcaccg caacaacgtc tgaagtatcc agatacagtt ccataaccat cctcaaaatg
    10021 tactttttgt ctgaatgtag actatcacgg gaaaactctc tgaacagttg acgcacatca
    10081 atctaacttt cattcgaaag tattttaatg tttgaacgaa acagagaggc gactatgatt
    10141 ttcaatattc agcgctactc gacccatgat ggccccggta tccgcacggt cgtatttctt
    10201 aaaggctgtt cgctgggctg ccgctggtgt cagaacccgg aaagccgcgc ccgcacgcag
    10261 gatctgctgt atgacgcacg actgtgtctg gaaggctgcg agctgtgcgc taaggccgcg
    10321 ccggaagtga ttgagcgcgc gctgaatggt ttgcttattc atcgggaaaa gttaaccccg
    10381 gagcatctga cggcgttaac cgactgctgt ccgacacagg cattaaccgt gtgtggtgaa
    10441 gtgaaaagcg ttgaggagat catgacgacc gttctgcgcg ataaaccgtt ttacgatcgc
    10501 agcggcggcg gtttaacgct ttcgggtggt gagcccttta tgcagccgga aatggcgatg
    10561 gcgctactgc aagccagcca cgaggcaggc attcatactg cggtagaaac ctgtctgcat
    10621 gtgccgtgga aatatatcgc cccttctctg ccctatatcg atctgtttct tgccgattta
    10681 aaacacgttg ccgacgcgcc gtttaaacag tggaccgacg gtaacgccgc cagagtgctg
    10741 gataacctga aaaaactcgc cgcagcgggc aaaaaaatca ttatccgcgt gccgctgatt
    10801 cagggcttta atgccgacga aacctctgta aaagctatta ccgattttgc cgccgacgag
    10861 ctgcacgttg gcgaaattca tttcctgccc taccacacgc taggcatcaa caaatatcac
    10921 ttacttaatc tgccctatga cgccccggaa aaaccgcttg atgcgccaga actgctcgac
    10981 tttgcccagc agtatgcctg ccagaaaggg ttaaccgcga ccttacgagg ataacaacca
    11041 tgaccacact gaaactggac acgctcagcg accgcattaa agcgcacaaa aatgcgctgg
    11101 tgcatattgt gaaaccgcca gtctgtaccg agcgcgcgca gcactatacc gagatgtatc
    11161 aacaacatct cgataagccg atcccggtac gtcgcgcgct ggcactggcg catcacctgg
    11221 cgaatcgcac catctggatc aaacacgatg agttgatcat tggcaaccag gcaagcgaag
    11281 ttcgcgccgc gccgatcttc ccggaatata ctgtctcgtg gatcgaaaaa gagattgatg
    11341 atctggcaga tcgtcccggt gctggctttg cggtgagcga agagaacaaa cgcgttctgc
    11401 atgaagtgtg cccgtggtgg cgcggtcaga ccgtacagga tcgctgctac ggcatgttta
    11461 ccgatgagca aaaaggtctg ctggcgaccg gaatcattaa agcggaaggc aatatgacct
    11521 ccggcgatgc gcacctggcg gtgaatttcc cgctgctgct ggaaaaaggt cttgatggtc
    11581 tgcgcgagaa agtagcggaa cgtcgctcgc gcatcaatct gacggtactg gaagatttgc
    11641 acggcgaaca gttcctgaaa gcgattgata tcgtgctggt ggcagtcagc gaacacattg
    11701 aacgttttgc agccctggcg cgtgaaatgg ccgcgaccga aacccgcgaa agccgtcgcg
    11761 atgaactgct ggcgatagca gaaaactgcg atcttatcgc ccaccagccg ccgcagactt
    11821 tctggcaggc gctgcaactg tgttacttca tccaattgat attgcagatc gaatctaacg
    11881 gtcactcggt atcgtttggt cgtatggacc agtatctcta cccgtactat cgccgcgacg
    11941 ttgaactcaa ccagacactg gatcgcgaac acgccatcga attgctgcat agctgctggc
    12001 tgaaactgct ggaagtgaac aagatccgct ccggctcaca ctcaaaagcc tctgcgggaa
    12061 gtcctctgta tcagaacgtc accattggcg gacaaactct ggttgatggt caaccaatgg
    12121 acgcggtgaa tccactctct tacgctatcc tcgaatcctg cggtcgcctg cgttccactc
    12181 agcctaacct cagcgtgcgt taccatgcag gaatgagtaa tgatttcctc gacgcctgcg
    12241 tacaggtgat ccgttgcggc ttcgggatgc cggcgttcaa caacgacgaa atcgtgatcc
    12301 cggaatttat taaactcggt attgaaccgc aggacgctta cgactacgca gcgattggtt
    12361 gtatcgaaac cgccgtcggt ggcaaatggg gctatcgctg taccggcatg agctttatca
    12421 acttcgcccg cgtgatgctg gcggcgctgg aaggcgggcg tgatgccacc agcggcaaag
    12481 tgttcctgcc acaagaaaaa gcgttgtcgg caggcaactt taacaatttc gatgaagtaa
    12541 tggacgcgtg ggatacgcaa atccgttatt acacgcgtaa atcaatcgaa atcgaatatg
    12601 tcgtcgacac catgctggaa gagaacgtgc acgatattct ctgctcggcg ctggtggatg
    12661 actgtattga gcgagcgaaa agtatcaagc aaggcggcgc gaaatatgac tgggtttctg
    12721 gcctgcaggt tggcattgcc aacctcggca acagcctggc ggcagtgaag aaactggtat
    12781 ttgaacaggg cgcgattggt cagcaacagc tggctgccgc actggcggat gacttcgatg
    12841 gcctgactca tgagcaactg cgtcagcggc tgattaacgg tgcgccgaag tacggcaacg
    12901 acgatgatac cgtcgatacg ctgctggctc gcgcttatca gacctatatc gacgaactga
    12961 aacagtacca taacccgcgc tacggtcgtg gtccggttgg cggcaactat tacgcgggta
    13021 cctcgtctat ctccgctaac gtaccgtttg gcgcgcaaac tatggcaaca ccggacgggc
    13081 gtaaagccca caccccgctg gcagaaggcg caagcccggc ctccggtact gaccatcttg
    13141 gccctactgc ggtcattggc tcagtgggta aactgcctac ggcagcgatt ctcggcggcg
    13201 tgttgctcaa ccagaaactg aatccggcaa cgctggagaa cgaatctgac aagcagaaac
    13261 tgatgatcct gctgcgtacc ttctttgaag tgcataaagg ctggcatatt cagtacaaca
    13321 tcgtttcccg cgaaacgctg ctggaggcga aaaaacatcc ggatcagtac cgcgatctgg
    13381 tagtgcgtgt cgcgggctat tccgccttct tcaccgcgct ctctccagac gcacaggacg
    13441 atatcatcgc ccgtactgaa catatgctgt aatctcctcg gcccggcggc gtcgtcgccg
    13501 ggctaaatca ttcacatcat caatttcatc cttactttca ttcgaaatat aatttgtgct
    13561 ctgcgtcaca ttgttattac actgttacgg tttcattttc gagccaggag cacacctatg
    13621 agcgtaaaag ttatcgtcac agacatggac ggtacttttc ttaacgacgc caaaacgtac
    13681 aaccaaccac gttttatggc gcaatatcag gaactgaaaa agcgcggcat taagttcgtt
    13741 gtcgccagcg gtaatcagta ttaccagctt atttcgttct ttcctgagct aaaggatgag
    13801 atctcttttg tcgcggaaaa cggcgcactg gtttacgaac atggcaagca actgttccac
    13861 ggcgaactga cccgacatga atcgcggatt gttattggcg agttgctaaa agataagcaa
    13921 ctcaattttg tcgcctgcgg tctgcaaagt gcatatgtca gcgaaaatgc ccccgaagca
    13981 tttgtcgcac tgatggcaaa acactaccat cgcctgaaag ctgtaaaaga ttatcaggag
    14041 attgacgacg tactgttcaa gttttcgctc aacctgccag atgaacaaat cccgttagtg
    14101 atcgacaaac tgcacatagc gctcgatggc attatgaaac ccgtcaccag tggttttggc
    14161 tttatcgacc tgattattcc cggtctacat aaagcaaacg gtatttcgcg gttactgaaa
    14221 cgctgggatc tgtcaccgca aaatgtggta gcgattggcg acagcggtaa cgatgcggag
    14281 atgctgaaaa tggcgcgtta ttcctttgcg atgggcaatg ctgcggaaaa cattaaacaa
    14341 atcgcccgtt acgctaccga tgataataat catgaaggcg cgctgaatgt gattcaggcg
    14401 gtgctggata acacatcccc ttttaacagc tgacctctct accggagccg tgcgctccgg
    14461 ttttcccttc tttttacatc ctgtacatca catcaaatca ttaaacttgc attaacttat
    14521 ttataactta tattgtcgca tgcaaatcag aatggtttcg aatgaaagtt aatgagaggt
    14581 ggttatggct tctactttta ccagcgacac attgcctgcc gatcacaaag cagctatccg
    14641 tcagatgaag cacgcgctgc gggcgcagct tggcgacgtc cagcagatct ttaatcagct
    14701 aagcgatgac attgccacgc gagtggctga aatcaacgca ctcaaagcac agggcgatgc
    14761 cgtctggccg gtgctgtctt atgccgatat caaagcaggt catgttactg cagagcagcg
    14821 cgaacagatt aaacgtcgcg gttgtgcggt gataaaaggc catttccccc gcgaacaagc
    14881 gctaggctgg gatcagtcga tgctggacta tctggaccgc aaccgctttg acgaggtcta
    14941 caaaggcccc ggcgataatt tcttcgggac gctcagcgct tcacgtcccg agatttaccc
    15001 catctactgg tcgcaggcgc aaatgcaggc ccgccagagt gaagaaatgg cgaatgcgca
    15061 gtcgtttctc aatcgtctgt ggacatttga aagtgatgga aagcaatggt ttaacccgga
    15121 tgtgagcgtc atctaccctg accgtatccg ccgccgtccg cccggaacga cctccaaagg
    15181 tcttggagcg cataccgact ccggggcgct ggaacgctgg ctgcttccag cgtatcagcg
    15241 cgttttcgcc aacgtcttta atggcaatct ggcgcaatat gatccctggc atgcggcaca
    15301 tcgtacggaa gttgaagagt acacggtgga caacaccacc aaatgttccg tgtttcggac
    15361 attccaaggc tggacagcgc tctctaatat gctgcctggt caggggctgc tgcacgttgt
    15421 gcccattcct gaagccatgg cgtacgtact gttacgtccg ctgcttgatg atgtgccgga
    15481 ggatgaactg tgcggcgtag cgcccggaag agtgttgccg gtatcagagc aatggcatcc
    15541 actgttaatt gaggcgttaa ccagcattcc aaaactcgag gccggagact ccgtctggtg
    15601 gcactgcgac gtcatccatt ccgttgcccc cgttgaaaat caacagggtt ggggcaacgt
    15661 gatgtacatt cctgcggcac caatgtgcga gaaaaatctt gcctacgcgc acaaggtgaa
    15721 ggccgcactg gaaaaaggcg catcgccggg cgacttcccg cgcgaggact atgaaacaaa
    15781 ctgggaagga cgctttacgc ttgccgacct caacattcac ggtaagcgag cgttgggaat
    15841 cgacagctga gtgtcgtaca gcccttcccg ttccacggcg gtgttccctt ttcctgggcg
    15901 aacccgccgc actgactccc gcaccgccaa tcgtccgtgc agcagcagcg agcccaccgg
    15961 agcatccggg cgaatcagcc gctgttgcaa catttgcacc gcttcattgc ctagctcatc
    16021 gcgtggcaca tgcacagccg tcaggggaac atcctcgatt gccgcaaggt taaagccgtc
    16081 gatactcatc accgagacat cctgtggtac gcgcagacca cgacgatgta gcgcatttac
    16141 cgttcccgcc gccataaaat ccccgccgac cagaaacgcc gtcggcaacg tcttgccatt
    16201 ggcggcatcc agccacgctc cgaccttttc ttccgcttct ttagcgctaa aactgctgat
    16261 attcagcaaa tgacggtcat ggtgaaaggc cagattatgc tgacgccatg attctttaat
    16321 ccctgcaaac cgctgctcca tagtgtagcg gcgcaggcac atcacattca tcacctcccg
    16381 gtgacccatt tcaaacagat agcgggcggc gaacgcgcca attaaccgat gatccggcgc
    16441 aatcgccggg aggcgcattc gttcatcacg gcagttgatc agcacacaag gcttagcgag
    16501 atcggcggcc agatcatgga tgtgcggatc gtcaatcccc agcaggatcg ctgcctgcgt
    16561 atctgcttca ttcatccgcg agagaaagag cgcgggatcg ctgtcaaact cctccagcgc
    16621 gcagtaacgc agccgcactt catgcggcga taacgctttg ctgatgctct ggatcacacg
    16681 gtaataaaag atatcagatc gttggtcaaa agcgcgttgc ggcgcgaaaa taatcaggtt
    16741 gttcagcaac atacgacctg ctgccatgcc atccatcacg cccaactcac gcgcgcaggc
    16801 cagcacctgt tcgcgagcgc gtgcactggt attcgctttg ccagccagca cccgcgaaac
    16861 cgtactcggc gatagcccgg tacgtgcggc gatatcggcg attttcagct tcttgtccat
    16921 tttgtgatct gcctctaatt cggaaatgac agaattttca tgagcaaaaa agcccattct
    16981 gtctggcatt aaatccattt cagtaatgta tatcagtcac tcatggaaaa acgtgcacaa
    17041 aacccgcttt ctttcgcttt tagggtctct tactgtgaat tggtagaaca acttccatac
    17101 aaaaataatg gaaaaacgaa caataaagac tttatttatc aaaaagttaa aagtggtgta
    17161 acaactccat tctgacgttg ccgcacaaac cctataccat tctgtggagt gcatatgagt
    17221 cagggtatca ataacgatat gactgccagt aaaccgcgcc atatcgtcaa aaatctgcgt
    17281 tggtggatac tggtcctttt tttgctcggt gttactgtca actacatcac gcgtaactcg
    17341 ctgggtattc tcgcccccga actgaaaagc agcctaggga ttaccaccga acaatattcc
    17401 tggattgtag gtgctttcca gctcgcctat accctattcc aaccgttgtg cggatggctg
    17461 attgacgtca ttggcctcaa actgggcttt atgatctgcg cgggactgtg ggcactggcc
    17521 tgtctgatgc atgcgggggc gggaaactgg ggacatctgg cgctgttgcg cttctttatg
    17581 ggcggtgcgg aagccgcggc aaccccagca aacgccaaaa ccatcggtga atggtttcca
    17641 aaatcagaac gcccgatcgc cgcaggctgg gcaggcgtcg gtttttccat cggtgccatg
    17701 ctggccccac cgattatcta ttttgcccac gcttcattcg gctggcaggg tgcatttatg
    17761 ttcactggca tcctggcgat gctgtgggtg gtgctgtggt gggcgttcta tcacagcccg
    17821 gataagcatc ctaacctcag caaagccgag ctggacttta ttcgccagga taacgaagcg
    17881 cccccggtca agttaccctt tctgaccgca ctgaaaaccg tgggcaaaaa taaacgcttt
    17941 tacggtatcg ctatcccggc ctttatggca gaaccggcgt gggcggtgat gagtttctgg
    18001 gtgcctctat acctgtccaa aacctatggc atggacctga agcagatcgc attattcgcc
    18061 tggttgccgt tcctcgccgc tgatcttggc agtattgcca gcggctatct gaccaaactt
    18121 tacacccgcc tgtttgggct gagtcgtgtg aactccgttg tcgcatcatc ggtcagtggc
    18181 gcattcttga tggtttcgct ggcggtgatg gcctttacca ataacccgta tatcgccatc
    18241 gtccttatct ccatcggcgg cttcggtcac cagatcatct cctgcatgct gagcgcactg
    18301 gtggtggaat ccttcgacaa aggtcagatg gccactgtta acggcatgcg cggttctgcc
    18361 gcgtggatcg caagtttcct gttctccctg attatcgggg ttaccgctga caaaatcggc
    18421 tttaaccccc tgtttatcgc catgggcttc tttgacctga ttggcgcttt cttcctggtc
    18481 acctttattg ctgaacgtcg cgcaaaacgc gcctgagagt ggatgtaaac atgaaaacgc
    18541 tgaaaaactg gaccctgcac aaacagtctg cccaccacgt tgaactgctg gtcgatggcc
    18601 agcatatcct gtgcctgtac gtgctggaag aaaatctgtt ccgcgtgctg ttaaaacgca
    18661 aaggcgagct ggcgctagat cgcacctgga gcattgctcc ggcgcaggat gtgccatggg
    18721 aagggcgcag ccgcgaagat ctcagcggct ttagcctgcc cgcgtggaca gtgatccagc
    18781 acgacgacac cgtggttatc gccaccgagt cactgcgtgc aaccgttcac cagccgctgt
    18841 ggctggagtg gcactatcgt aacaacgaag gcgaatggct gccgttggtg aatgaccgcc
    18901 cgaccagcgc ttatctgctg aatgcccacg gcgatggcgt ggcacactat cagagccgtc
    18961 gtaaagatga acgcttttat ggcctcggag ataaagccgg cgatctgcaa cgcaacggta
    19021 aacgttacga gatgcgtaac ctcgacgcga tgggctacaa cgccgttagt actgacccgc
    19081 tgtacaaaca cattccattc accatcaccc gccgcgatga cattagcttc ggcctgtttt
    19141 atgacaacct cagcagctgc tggctggatc tgggcaacga gatcgacaac taccacaccg
    19201 cctatcgccg ctggcaggcg gaagcgggtg atatcgacta ctacctgttt accggcgaac
    19261 gcgtacttga tatcaccaaa gccttcgtac gcctgaccgg aaaaaccctg ttcggaccga
    19321 aatggagcct gggctacagc ggttcaacta tgcactacac cgatgcgccg gacgcacaaa
    19381 atcagttgat gaactttatc cgcctgtgtg aagaacatgc aattccctgc gattcgttcc
    19441 agctctcttc cggttatacc tcaattaacg gcaaacgcta tgtgttcaac tggaactacg
    19501 acaaagtgcc gcagccgaaa gtgatgagcc aggcatttca caatgctggt ttgcacctgg
    19561 ccgccaatat caagccgtgc ctgttgcagg atcacccacg ctataacgaa gtggcagaaa
    19621 gaggactgtt catccgtgat tcagaaacag atgaaccaga acgttccagc ttctgggatg
    19681 acgaaggttc taacctcgag tttactaatc cgcagaccat ccagtggtgg caggaaggcg
    19741 tgaccacaca gcttctggag atgggtatcg actccacctg gaacgacaac aacgaattcg
    19801 aagtatggga cggtgaagca cgctgtcacg gctttggtca gacgatcgcg atcaaacata
    19861 ttcgcccagt gatgccactg ctgatgatgc gagcctcgat ggaagcccag cagcgtttcg
    19921 ctccggaaaa acgtccgtac ttgatctccc gctccggctg cgcgggaatg cagcgctacg
    19981 tgcagacctg gagtggcgat aaccgcacta gctgggacac tctgcgctac aacacccgca
    20041 tgggtctggg gatgagcctc tccgggctgt ataacctcgg tcacgacgtc ggcggtttct
    20101 ccggtgataa gccggacccg gaactgtttg tacgctgggt acaaaacggc gtgatgcatc
    20161 cgcgctttac gattcactca tggaacgatg accataccgt aaacgaaccg tggatgtatc
    20221 cgggcgtcac tccggcgatc cgcagcgcta ttgagttgcg ctatcgcctg ctgccctact
    20281 tttataccct gatgtggcag gcatacgccg atgatgaacc aatgctacgt ccgactttcc
    20341 ttgaccacga gcacgacgtt cagacttttg aagagtgtga tgacttcctg cttggtcgcg
    20401 atattttggt tgccagcgtc gtagagcaag gcgagcgcca gcgccgtgta tggctgccag
    20461 ataacgaaac tggctggtac gatttctaca acggcgaatg gttcagtggc ggtcagtgga
    20521 tcaccctcga cgcaccgctg gagaaactgc cgctgatggt tcgtgccggg gctggtctac
    20581 cgctcagcaa acgcatcacc tatgtgagcg cagaacaaga cgatacccgc gaactgaaac
    20641 tgttcccgct gaaaggtgtg ggcacaacgt ccggcctgct gtttgaagac gatggcgaaa
    20701 gctggggcta ccaaactggt aatgcgcttt gggtggaatg ggaaatggtt tgcgatgggg
    20761 cgaccatcaa cctgaaagtc aacgcgcgcg gggattatcg tcctgcatgg agtgcactga
    20821 aagtgtcatt accattggaa gaaaaacgca cgctgctggt caatggtgtt gaagggagtg
    20881 aatggatgcg ttaaatcatt accggggccg ggaggcgact gcaccttacc cggtccagcc
    20941 atcgaatcct ttacctcagt atttcacgct gcaggaaggc agcaaacctg taaaccccgg
    21001 gagcttaaat aagtaagtga ccggggtgaa caggtgaagc caatgcatct gccgcatgaa
    21061 agacggcggg gaattaagcg tcgatccctt tactacgcag gtaatcttcg taattaccgc
    21121 taaagtcgat cgcgcgttcc ggggtgattt ccagaatgcg ggtcgccagg gagcttacga
    21181 actcacggtc gtgtgaaaca aagatcagcg tgccctgata cagttccagc gccatgttca
    21241 gtgactcgat ggattccata tccaggtggt tggtcggttc gtccatgatc agaatgttcg
    21301 gcttctgcat cattaactta ccaaacagca tccgcccttt ttccccaccg gaaagcactt
    21361 tagctggctt tttgatgtcg tcctggctga acagcaaacg accgagaatg ctgcgtaccg
    21421 cctgctcgtc atcgccttcc tgcttccact gactcatcca ttcgaacacg gtcagatcat
    21481 tttcaaactc atattcgtgg tcctgggcgt agtaaccaat gcgcgcgttt tcagaccatt
    21541 ttacggtgcc gctgtccggt tgcagatcgc ccaccagcgt tttcagcagc gttgatttac
    21601 cgacgccgtt ggtacccagt accgcaagtt tttcaccgac ctccagcagc aggttgagat
    21661 ttttaaacag tggaccgtta tcaaaccctt tggtcagacc ttccacttcc agcgcgttac
    21721 ggaacagttt cttatcctgt tcaaaacgga tgaacgggtt ctgacggctg gaggctttca
    21781 cctcttccag tttgatttta tcaatctggc gcgcgcgcga agttgcctgg cgagatttcg
    21841 aggcgttggc gctaaagcgg ctaacgaaag attgcaactc agcaatctgc gctttcttct
    21901 tggcgttatc ggccagcaga cgttcacgcg cctgggtcgc cgccgtcatg tactcatcgt
    21961 agttacccgg ataaacgcgc agctcgccgt aatccagatc cgccatgtgg gtacagacca
    22021 tgttaaggaa gtgacggtcg tgcgagatga tgatcatggt gctgtcacgc tcgttcagca
    22081 cctgttccag ccagcgaatg gtgtcgatgt cgaggttgtt ggtcggttcg tcgagcagga
    22141 gaatctccgg atcagcaaac agcgcctgcg ccagaagcac acgcagtttc cagccaggag
    22201 caacttcact catcgggccg tagtgctgtt ccactggaat tcccacgcca agcaacagtt
    22261 caccggcgcg agcttccgca gagtaaccgt ccatttcgcc gtatttaact tccagatcgg
    22321 ccactttata gccgtcttcc tcactcattt ccggcaaagc atagatgcgg tcacgctcct
    22381 gcttcacttc ccacaactct ttatgcccca tgatcaccgt atccagcaca gtgaactctt
    22441 caaaggcaaa ctgatcctga cgcagcttac caatgcgctc gttgggatcg agggaaacgt
    22501 tacccaaagt cggctctaag tcgccgccga ggatcttcat aaaggtggat ttaccactac
    22561 cgttcgcgcc aatcaggccg taacggttgc cgccgccgaa tttgacggaa atgttttcaa
    22621 acaacggctt actgccgaac tgcatggtga cgttactgga aactaacaca ggcgtatcct
    22681 gaaaagagat atgacaaacc gcgcattatg ccataagtct gaaagaagat cctgctcctc
    22741 gcttaaggtc tacactcaag ccaaaggcaa aaaatgtgat ttcgtacaca tctgatttca
    22801 ctgtgagctg gaatgaactt ataatgcgct tccaatactc taatattctc aacccaatgg
    22861 cctgccaggc acaaaatctc gcttaacatg aatatgaaat tgaaaacatt attcgcagcg
    22921 gccttcgctg ttgtcggctt ttgcagtacc gcctctgcgg taacttatcc tctgccaacc
    22981 gacgggagtc gcctggttgg tcagaatcag gtgatcacca ttcctgaagg taacactcag
    23041 ccgctggagt attttgccgc ggagtaccag atggggcttt ccaatatgat ggaagcgaac
    23101 ccgggtgtgg ataccttcct gccgaaaggc ggtactgtac tgaacattcc gcagcagctg
    23161 atcctgccgg ataccgttca tgaaggcatc gtcattaaca gtgcagagat gcgtctgtat
    23221 tactatccga aagggaccaa caccgttatc gtgctgccga tcggcattgg tcagttaggc
    23281 aaagatacgc ctatcaactg gaccaccaaa gttgagcgta agaaagcagg cccgacctgg
    23341 acgccgaccg ccaaaatgca cgcagagtac cgcgctgcgg gcgaaccgct tccggctgtc
    23401 gttccggcag gtccggataa cccgatgggg ctgtatgcac tctacatcgg tcgcctgtac
    23461 gctatccatg gcaccaacgc caacttcggt atcggcctgc gtgtaagtca tggttgtgtg
    23521 cgtctgcgta acgaagacat caaattcctg ttcgagaaag taccggtcgg tacccgcgta
    23581 cagtttattg atgagccggt aaaagcgacc accgagccag acggcagccg ttatattgaa
    23641 gtccataacc cgctgtctac caccgaagcc cagtttgaag gtcaggaaat tgtgccaatt
    23701 accctgacga agagcgtgca aacagtgacc ggtcagccag atgttgacca ggttgttctt
    23761 gatgaagcga ttaaaaaccg ctccgggatg ccggttcgtc tgaattaatc ttcaaaactt
    23821 aaagcaaaag gcggactcat aatccgcctt ttttatttgc cagaccctag ttggccggga
    23881 gtataactaa caaaacatat cccaccaacg ccgcccacaa cagcatcggt attgaataga
    23941 gatggaaacg ccaccagatg cggcgatcgt tcgccatacg tagcgcaatc aaatttgcca
    24001 gcgatccggg taataaccca aagccaccta cgtttaccgc ccataccagt aataaagacg
    24061 gcggcacata gttcagcaac aatatggtac tcggcacgtt actgatcacc tgcgataaac
    24121 cgattgccgt taaccataac ccgggttcag atagatgact cacgttaccc aacacgcctt
    24181 gcaacgctgg aagctgggtc agtaaatgga cgtcgataaa catcgccata aacaccagca
    24241 gcagcgtcca gtccacactg agcaccacgc gacgtgccag cagcgcaaag cctgccgcca
    24301 caatcaccag tccccacagc tcttgtttga actccagcgc cgtcagaaag acgatataca
    24361 gccccagaca actccacacc agccgcggtt tccactccgg tgtttgcacc cccgtatggt
    24421 attgcagcgc ctttccaggg aaacaacacc agcacaggag caggagcgtc agcatcattg
    24481 cgccagccag cggtgccatt tgggcaataa atccggcaaa cgaaagacca gaacgtcccc
    24541 agataagaat attttgcggg ttgccaattg gcgtcagtag cgaaccagcg ttgactgcca
    24601 gcgcctcaaa aataatcagc cgattaaccg ggatctcaca cagtcttttt agcgtgatag
    24661 tcagcggaac aacaataaac agcgcgacat cgttggtcag aaaggtagaa agcagcgccg
    24721 ccgccagcac cataaacatc gccagccgac gctccgtagc aaagcggcgc accattttgc
    24781 gccccagcac atcaaaataa ccgcttaact ccacaccttt ggtcagcagc atcaggccgc
    24841 ttaaggtgat gatggtgtgc cagtcgatag cagcaggcca ggatttcggt gcaaagggca
    24901 cgaaaaagct taatccgata ccaacaagaa ttaataactg aaaaaaacga tcgccttgca
    24961 gcgtgcgtaa aaaaggcagg ctcatttggc accgtgtttc tgggtaaaca aacgaaaagc
    25021 gtcgagcgtc tcttcactaa catggtgctc catgccttcc gcgtcgcgac gggcgatttc
    25081 cggactgacg cccaacacca acaagaaatt ttcgactatc tgatgtcgct cgcggctttc
    25141 ctgcgccagc ttctctcctt ctgccgttaa aaacacgcca cgccagggga tcatttcaat
    25201 cagccccatg gttgccagcc gcttaagcat tttagccacc gtcggttgcg aaactcccag
    25261 acgagcagcc atgtccacct gacgagcttc ccccacttcc ctgatcaagt cagaaatcag
    25321 ctcaacgtaa tcatcaataa gctcgcgccg atgcgcctct ctgacctggc ggaacccttc
    25381 aacgtgctct tccacgttca ctaactgcgt cacttttttt gctgttggcg tacctgcgcg
    25441 acgactcatt gtgcttcctc attatggtga cgcatacata gcgttctcat ttcgtgcgca
    25501 cattgtaaac cagagttgcg aaggtacaaa aaattaacgt tttagcaata gctacataat
    25561 atagcctgtg ctatatctgt atgtaatgca atcatccctc aaggatcgac gggattagca
    25621 agtcaggagg tcttatgaat gagttcaaga agtgtatgcg cgtgtttagt cattctccct
    25681 ttaaagtacg gttaatgctg ctctctatgt tgtgcgatat ggtcaacaac aaaccgcagc
    25741 aagataaacc ttccgataaa tagcggcgtc gcggtacgcc gcttcactcc tgcttttatg
    25801 caggcataac gcgttttggt ctgaaaaacc ccactttttg tcggatttgc aatccccttc
    25861 gcaaaagatt tgttcgtcag tagttgacct gaacggcggc tcgctctatc ttcttgcagc
    25921 cctacgtata ttgcggctcg cggatgcgga cccctttccc ctcttcacgc actcttgcag
    25981 gtattgaccc ttgacgccag ggtaagcaca tggcgtttgt tacgatagtg gcatatgaat
    26041 ttaaccctca aagaatcgct tgttacccgt agccgggtat ttagcccgtg gactgcgttc
    26101 tactttttac agtcgctatt aattaacctc ggcttaggtt accccttcag tttgctctac
    26161 accgctgcgt ttacggctat tttgcttttg ctatggcgaa cattgcctcg cgtacaaaaa
    26221 gttctggtcg gtgtcagttc gctggtggcg gcttgttatt tcccttttgc tcaggcctac
    26281 ggcgcgccta atttcaatac attgctggca ttgcactcca ccaatatgga agagtcgacc
    26341 gaaatcctga cgatttttcc gtggtacagc tacctggtcg gcttatttat ttttgcgctc
    26401 ggcgtaatag caatcaggcg aaaaaaagag aatgaaaaag cgcgctggaa taccttcgac
    26461 agcctgtgtc tggtattcag tgtggcgaca ttttttgttg ctcccgtgca aaacctggcc
    26521 tggggtggcg tatttaaact gaaagatact ggctatccgg tatttcgttt tgctaaggat
    26581 gtcatcgtca ataataacga ggtgattgaa gagcaagaac ggatggcaaa actttccgga
    26641 atgaaagata cctggacggt cactgccgtt aagccgaagt atcagaccta tgtggtggtg
    26701 atcggcgaaa gcgcgcgtcg cgatgccctc ggtgcctttg gcggtcactg ggacaatacc
    26761 ccgtttgcca gcagcgttaa cggtttgata tttgctgact acattgccgc cagtggctcc
    26821 acgcagaaat cacttggctt aacgctcaat cgcgtagtcg atggcaaacc acagtttcag
    26881 gataactttg tcaccctggc aaatcgcgcg ggcttccaga cctggtggtt ttccaaccag
    26941 ggccaaatcg gcgaatacga tactgctatc gccagcatcg ccaaacgtgc agatgaagtg
    27001 tacttcctga aagaaggtaa tttcgaagca gataaaaaca cgaaagacga agcgttactg
    27061 gatatgaccg ctcaagtgct ggcgcaagag cactcgcaac cgcagctgat tgttctgcat
    27121 ctgatgggct cgcatccgca ggcctgcgac aggacacaag gcaaatacga aacctttgtg
    27181 caatcgaaag aaacgtcgtg ctatctctat accatgacgc aaacggacga tttactgcgc
    27241 aagctgtacg atcagttacg caacagcggc agcagcttct ctctggttta cttttctgac
    27301 cacggtctgg cctttaaaga gcgcggcaaa gacgtgcaat accttgccca tgatgataaa
    27361 tatcagcaaa atttccaggt gccttttatg gtcatttcca gcgacgataa agcgcatcgg
    27421 gtgattaaag cccgccgctc agccaatgac ttcttaggct ttttctcgca gtggacggga
    27481 attaaagcga aggaaataaa tatcaaatac ccgtttatat ctgagaagaa agccgggccg
    27541 atatacatca ccaacttcca gttacagaag gtggattaca accatctcgg aaccgatatt
    27601 ttcgacccga aaccttaaaa caaaaaatcc gccccgagag gcggattttt tatatcacca
    27661 aagtgattag aagcggtaac caacaccggc aatccaggtg cctacgtcaa cgctacgaat
    27721 acggctctgc tcgtaagaga agtccagagc aacgttttcc atcgggttga actgcagacc
    27781 agcaccgtag gagaaaccgt agtcgctggt gtcgtgtttg taggtcgggt attcagtggt
    27841 ctggaattta ccataaccca cccccactac accgtagatg cttgcccagt cgttaatgcg
    27901 gtaagccgga ccagcagtga tgccgtagta ctggtttttg ttgtagtcac cagagcttgc
    27961 agtacggctt ttctcggtgt aagtgaaaga accgatcaca cccagcgggc tgttgtcttc
    28021 ttcatagcgg tatttcaggt tgaaaccgcc cattttgttc atttggccct gagcgtcgct
    28081 ctgtgcgtaa ccgccagtta cggtagaagt cgcagctacg gaagtacctg cggtgaaagc
    28141 cagaactgcg gccagtgctg aaagacatgc aatttttttc ataaccacct caaatgtgat
    28201 tcaaataagt cctaagtttt aaatatatca aaaattaatg ggaaactctt cgcgatttgt
    28261 gatgtctaac gggccatttc atgtaacaga acgtttccat acaccgctat ccatctaaat
    28321 ttaaatcact ttttcagaga actgcgtaag tattacgcat gttttccctg tcattcatcc
    28381 agattattcc taatcaccag actaatgatt ccatcaatcc tggcgcattt tagtcaaaac
    28441 gggggaaaat tttttcaaca aatgctcaac cagcattggg tatatccagt acactccacg
    28501 ctttacttaa gtctagatat ttgtgggaga aaggatgcct ggttcattac gtaaaatgcc
    28561 ggtctggtta ccaatagtca tattgctcgt tgccatggcg tctattcagg gtggagcctc
    28621 gttagctaag tcactttttc ctctggtggg cgcaccgggt gtcactgcgc tgcgtctggc
    28681 attaggaacg ctgatcctca tcgcgttctt taagccatgg cgactgcgct ttgccaaaga
    28741 gcaacggtta ccgctgttgt tttacggcgt ttcgctgggt gggatgaatt atctttttta
    28801 tctttctatt cagacagtac cgctgggtat tgcggtggcg ctggagttca ccggaccact
    28861 ggcggtggcg ctgttctctt ctcgtcgccc ggtagatttc gtctgggttg tgctggcggt
    28921 tcttggtctg tggttcctgc taccgctggg gcaagacgtt tcccatgtcg atttaaccgg
    28981 ctgtgcgctg gcactggggg ccggggcttg ttgggctatt tacattttaa gtgggcaacg
    29041 cgcaggagcg gaacatggcc ctgcgacggt ggcaattggt tcgttgattg cagcgttaat
    29101 tttcgtgcca attggagcgc ttcaggctgg tgaagcactc tggcactggt cggttattcc
    29161 attgggtctg gctgtcgcta ttctctcgac cgctctgcct tattcgctgg aaatgattgc
    29221 cctcacccgt ttgccaacac ggacatttgg tacgctgatg agcatggaac cggcgctggc
    29281 tgccgtttcc gggatgattt tcctcggaga aacactgaca cccatacagc tgctggcgct
    29341 cggcgctatc atcgccgctt caatggggtc tacgctgaca gtacgcaaag agagcaaaat
    29401 aaaagaatta gacattaatt aaatttacat ttctgcatgg ttatgcataa ccatgcagaa
    29461 tttctcgcta cttttcctct acaccgtctt tatatatcga attatgcaaa agcatattta
    29521 ttccgaaaat tcctggcgag cagataaata agaattgttc ttatcaatat atctaactca
    29581 ttgaatcttt attagttttg tttttcacgc ttgttaccac tattagtgtg ataggaacag
    29641 ccagaatagc ggaacacata gccggtgcta tacttagtct cgttaattac tgggacataa
    29701 catcaagagg atatgaaatt atgagtaccg ctaaattagt taaatcaaaa gcgaccaatc
    29761 tgctttatac ccgcaacgat gtctccgaca gcgagaaaaa agcaacagta gagttgctga
    29821 atcgccaggt tatccagttt attgatcttt ctttgattac caaacaagcg cactggaaca
    29881 tgcgcggcgc taacttcatt gccgtacatg aaatgctgga tggcttccgc accgcactga
    29941 tcgatcatct ggataccatg gcagaacgtg cagtgcagct gggcggtgta gctctgggga
    30001 ccactcaagt tatcaacagc aaaaccccgc tgaaaagtta cccgctggac atccacaacg
    30061 ttcaggatca cctgaaagaa ctggctgacc gttacgcaat cgtcgctaat gacgtacgca
    30121 aagcgattgg cgaagcgaaa gatgacgaca ccgcagatat cctgaccgcc gcgtctcgcg
    30181 acctggataa attcctgtgg tttatcgagt ctaacatcga ataaatccat cgctgatggt
    30241 gcagaacttt agtacccgat aaaagcggct tcctgacagg aggccgtttt gttttgcagc
    30301 ccacctcaac gcacttattt agtgcatcca tctgctatct ccagctgatt aagtaaattt
    30361 tttgtatcca catcatcaca caatcgttac ataaagattg ttttttcatc aggttttacg
    30421 ctaaataatc actgtgttga gtgcacaatt ttagcgcacc agattggtgc cccagaatgg
    30481 tgcatcttca gggtattgcc ctataaatcg tgcatcacgt ttttgccgca tctcgaaaaa
    30541 tcaaggagtt gcaaaactgg cacgattttt tcatatatgt gaatgtcacg caggggatcg
    30601 tcccgtggat agaaaaaagg aaatgctatg aagtctgtat taaaagtttc actggctgca
    30661 ctgaccctgg cttttgcggt ttcttctcat gccgcggata aaaaattagt tgtcgcgacg
    30721 gataccgcct tcgttccgtt tgaatttaaa cagggcgata aatatgtggg ctttgacgtt
    30781 gatctgtggg ctgccatcgc taaagagctg aagctggatt acgaactgaa gccgatggat
    30841 ttcagtggga tcattccggc actgcaaacc aaaaacgtcg atctggcgct ggcgggcatt
    30901 accatcaccg acgagcgtaa aaaagcgatc gatttctctg acggctacta caaaagcggc
    30961 ctgttagtga tggtgaaagc taacaataac gatgtgaaaa gcgtgaaaga tctcgacggg
    31021 aaagtggttg ctgttaagag cggtactggc tccgttgatt acgcgaaagc aaacatcaaa
    31081 actaaagatc tgcgtcagtt cccgaacatc gataacgcct atatggaact gggcaccaac
    31141 cgcgcagacg ccgttctgca cgatacgcca aacattctgt acttcatcaa aaccgccggt
    31201 aacggtcagt tcaaagcggt aggtgactct ctggaagcgc agcaatacgg tattgcgttc
    31261 ccgaaaggta gcgacgagct gcgtgacaaa gtcaacggcg cgttgaaaac cctgcgcgag
    31321 aacggaactt acaacgaaat ctacaaaaaa tggttcggta ctgaaccgaa ataataacgc
    31381 tacacctgta aagcgcactg gcagttccct ctcccctctg gggagaggat tagggtgagg
    31441 ggcgcaaacc cgctccgggg ccattaatta ccctgaattt gattatttac accacggtaa
    31501 caggaacaac atatgcagtt tgactggagt gccatctggc ctgccattcc gcttctgatt
    31561 gaaggtgcca aaatgaccct gtggatttcg gtcctcggtc tggctggcgg tctggtaatc
    31621 ggattgctgg caggttttgc acgcaccttc ggtggttgga tagccaacca cgtcgcgctg
    31681 gtctttattg aggtgatccg cggcacacct atcgtcgtcc aggtgatgtt tatttatttc
    31741 gccctgccga tggcgtttaa cgacttacgc atcgacccat ttactgcggc ggtggtcacc
    31801 atcatgatca actccggcgc gtacattgcg gaaatcacgc gtggtgcggt gctgtctatt
    31861 cacaaaggtt ttcgtgaagc aggactggcg ctcggtcttt cacgttggga aaccattcgc
    31921 tacgtcattt taccgctggc actgcgtcgt atgctgccgc cgctgggtaa ccagtggatc
    31981 atcagcatta aagacacctc gctgtttatt gtgatcggcg tggcggaact gacccgtcag
    32041 gggcaagaaa ttattgccgg taacttccgc gcccttgaga tctggagcgc cgtggcggtg
    32101 ttctatctga ttattaccct ggtgctaagc tttattttgc gtcgtctgga aagaaggatg
    32161 aaaatcctgt gattgaattt aaaaacgtct ccaagcactt tggcccaacc caggtgcttc
    32221 acaatatcga tttgaacatt gcccagggcg aagtcgtggt gattatcggg ccgtccggtt
    32281 ccggtaaatc gaccctgctg cgctgcatca acaaactgga agaaatcacc tccggcgatc
    32341 tgattgtcga tggcctgaag gttaacgatc cgaaagttga cgagcgcctg attcgccagg
    32401 aagcaggtat ggtgttccag cagttttacc tcttcccgca tctgacggcg ctggaaaacg
    32461 tcatgtttgg tccgctacgc gtgcgtggcg cgaacaaaga agaggcggaa aaactggcgc
    32521 gtgagctgct ggcgaaagtc ggtctggcag aacgtgcaca tcactaccct tccgaacttt
    32581 ctggtggtca acagcagcgt gtggcgattg cccgcgcgct ggcggtgaag ccgaaaatga
    32641 tgctgtttga tgaaccgact tccgctcttg acccggaact gcgccatgaa gtgctgaagg
    32701 ttatgcagga tctggctgaa gaagggatga cgatggtgat cgtgacccac gaaatcggtt
    32761 ttgccgagaa agtagcttcg cggctgatct ttatcgacaa aggccggatt gcggaagatg
    32821 gcgatccgca ggtattgatc aagaacccgc cgagccagcg cttgcaggaa tttttgcagc
    32881 acgtctctta acttctctgc ctccctttca cccgaaaggg aggcacacca gattcctctc
    32941 atttaaaatc gcccctcccc cagcatctat acttatcttt ttgctctgtt ttctcactgg
    33001 aggagtcatg cggtggatcc tgttcatcct cttctgcctg ctgggcgcac ctgcccacgc
    33061 ggtatccata cccggcgtta caaccacaac gacaacggac tcaacgactg aaccggcccc
    33121 ggaaccggat atcgaacaaa aaaaagcggc ctatggcgca ctggcggatg tgctggataa
    33181 cgacacctcg cgtaaagaat tgatcgacca gttgcgtact gtcgccgcga cgccccctgc
    33241 ggaaccggtc ccgaagatcg tgccgccgac gctggttgaa gagcaaaccg tgctgcaaaa
    33301 ggtcaccgaa gtcagccgcc attatggtga agccctttcc gcccgcttcg ggcaacttta
    33361 tcgcaatatc accggctccc cgcataagcc atttaatcca caaaccttca gcaatgcgct
    33421 gagccatttt tcaatgttag cggtattagt gtttggtttt tactggctga ttcgcctgtg
    33481 cgcactgccg ctgtatcgca aaatgggtca gtgggcgagg caaaaaaatc gtgagcgcag
    33541 caactggttg cagcttccgg cgatgattat cggggcgttt attatcgacc tgctgttact
    33601 ggccctgaca ttatttgtcg gccaggtatt aagcgacaac ctgaatgcag gcagtcgcac
    33661 catcgctttc caacaaagtt tgtttctcaa cgcctttgcc ctgattgaat ttttcaaagc
    33721 cgtactacgc ctgatttttt gcccaaacgt ggcggagctg cgcccgttca cgattcagga
    33781 cgagagcgcc cgttactgga gtcgtcgcct gagctggtta agcagtttga taggttatgg
    33841 cctgattgtg gccgtgccga ttatctctaa tcaggtgaat gtacagatag gtgcgctggc
    33901 gaacgtcatc attatgctgt gcatgaccgt ctgggcgttg tacctgatct ttcgtaataa
    33961 aaaagagatt acccagcatt tgctcaactt tgcggagcat tcgctggcct ttttcagcct
    34021 gtttatccgc gcctttgcgc tggtgtggca ctggctggca agcgcctatt ttatcgtgct
    34081 gtttttcttt tcgttgttcg atccgggcaa cagcctgaaa tttatgatgg gtgcaacggt
    34141 gcgcagcctg gcgattattg gtatcgcagc gtttgtttcc ggtatgtttt cccgctggct
    34201 ggcaaaaacc atcactctct cgccacatac tcagcgtaac tatccagagt tgcaaaaacg
    34261 gctgaatggc tggctttcgg cggcgctgaa aacggcgcgt attctgaccg tctgcgtggc
    34321 ggtaatgttg ctgttgagcg catggggatt gttcgatttc tggaactggc tgcaaaacgg
    34381 cgcggggcag aaaaccgtag atatcctgat ccgtatcgca cttattcttt ttttctcggc
    34441 ggttggctgg acggtgctcg ccagtttgat cgaaaaccgg ctggcttcgg atattcatgg
    34501 ccgcccgcta cccagcgccc gtacgcgtac cctgctgacg ctgttccgta acgcgctggc
    34561 ggtgattatc agtaccatca ccatcatgat tgtgttgtcg gaaatcggcg tcaatatcgc
    34621 gccattgctg gcaggtgccg gggcattagg tctggctatc tcgtttggtt cgcaaacgct
    34681 ggtgaaagat atcattaccg gggtgtttat tcagtttgaa aacggcatga acactggaga
    34741 tttggtgact atcgggccgt tgaccggcac agtggaacgg atgtcgattc gctccgtggg
    34801 cgtgcgccag gataccgggg cgtatcacat cattccgtgg tcttcgataa ccacctttgc
    34861 taacttcgtc cgcggtattg gttcggtagt ggcaaactac gatgttgatc gccatgaaga
    34921 tgctgataaa gccaatcagg cactgaaaga tgcggtagcg gaattaatgg aaaacgaaga
    34981 aattcgcggg ctgattattg gtgaaccgaa ttttgccggg attgtcggct taagcaatac
    35041 cgcgtttaca ctgcgtgttt cgttcaccac gctgccactc aaacagtgga cggtacgctt
    35101 tgccctcgac agccaggtga aaaaacattt cgacctggcg ggcgttcgcg cgccagtgca
    35161 gacttatcag gtgctgccta ctccgggcgc gaccccggct gaaccgttac cgccggggga
    35221 gccaacgctt taacgctggc gattgacaaa acggcggcgc tgctcgtcgt tcataaaggt
    35281 ccaggcaata aagcgactct gcttttgccc ctgggccatc tcttttttta ccaccttcac
    35341 cgcgcccacg tcggtcagag cacgatacaa cggcggtaag ttttcaccac gagataccag
    35401 cgaggtaaac cacatcacct gcttcgcgaa gcctttgctc tcttcaatca tctttttgat
    35461 aaaggcgact tcaccacctt cacaccacaa ctcctgttgc tgaccaccaa agttcagtgc
    35521 atcgtctttg ttcagcccca ggttacggcg tttacgctca ctacctgccc gcgctgcagc
    35581 ggcggaatcg tggaatggcg ggttacacaa ggtcgcgtcg tattgctcgt ttttatggat
    35641 gatgccgtta aaaattgccc cactctcttt ttgccgacgc agacgaatgg cgcggttaag
    35701 ccccggatta gcactgataa tcgcctgcgc actgcttaac gcctggctgc tggtttcact
    35761 accggtaaaa cgccagccat attcatgtac gccaatcagc ggataaatac agttcgcacc
    35821 aacgccgata tccagaatgc tggcattcgc tggaattgtt ccgcttgctt cagccagtaa
    35881 atcggcaagg tgatgaatat aatccgcccg gcccggtact ggtgggcaga gaaaaccgtc
    35941 ggggatatcc cagttcgcta cggcgtaaaa atgggccagc aacgccttat tgagcgcctt
    36001 caccgccagc ggattggcaa agtctacgct ttgctccccg gcgggtgtaa gcgtgaggaa
    36061 ttgcctgagt tcaggattga cctgacaaag cgtggcgaga tcgtagcggc tgtgatgacg
    36121 gttgcgcgga tgcaaccccg gtttctgggc ggacatagca ttctccttta agcagcggcg
    36181 taagataccc gcataacgcg acagggtaaa tatctggctg cgtttatcat tccccaaaac
    36241 cttaacatgg ctatggttta tggcaattca tcataaaaat cagcattatg catgtcatta
    36301 atttgtctta ttttgtaaaa atatatcgtt caaaaaataa gcacatctct gtcaattttt
    36361 tgcgtcattt gcatcgcgca ggcgacctac acttatcagg cactacccgt tgtatttatg
    36421 aggatgtcat tatgaaaaag tgcctcacac tactgattgc caccgtcctg agcggaatct
    36481 ctctcacggc ttatgccgcg caaccgatga gtaacctgga cagcggtcag ctacggcccg
    36541 ccggtaccgt ttcggcaact ggcgcatcaa acctaagcga tctggaggat aaactggcag
    36601 aaaaagcgcg cgaacaaggc gcgaaaggtt atgtcattaa ttccgcaggc ggaaatgacc
    36661 agatgttcgg tactgcaacc atctacaaat aaccccacca acgtaactgc attcgcctct
    36721 ggatgcagtt ttcgcctgtt ttgtaatcaa aaaacatcaa atatgaactc aatgtaaata
    36781 aatgtatttc tttttcgcgc aatgggtgat agaaaatcgc gccaagtgat aatgcttatc
    36841 aaaattatta tcacttgcat gaacactatc acgggattaa cagtggcatc gcatccgcag
    36901 agatgctttc tcgtggcagt gaaaatttca acatataaga aaaagtcacc tgcaaaatgg
    36961 aaaacaatcg caatttccct gccagacaat ttcattcgct cacgttcttt gccggtcttt
    37021 gtattggcat cacgcctgtg gctcaggcac tcgccgccga agggcaaact aacgcggatg
    37081 acacgctggt tgtcgaagca tcaacgcctt cgctttatgc gccacaacaa tctgccgatc
    37141 cgaaattctc gcgtccggta gcggatacta cccgcacgat gacggtgatt tctgaacaag
    37201 tgattaaaga tcagggcgca accaacctta ccgacgcgct caaaaacgcc cccggcg
//
LOCUS       DADMKF010000024        36764 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.024, whole genome shotgun sequence.
ACCESSION   DADMKF010000024 DADMKF010000000
VERSION     DADMKF010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36764
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.024"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(<1..135)
                     /locus_tag="JNC24_001094"
     CDS             complement(<1..135)
                     /locus_tag="JNC24_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HAY9157905.1"
                     /translation="MIIGNIHNLQPWLPQELRLAIEHIKAHVTAETPKGKHDIEGNRL
                     F"
     gene            complement(280..873)
                     /locus_tag="JNC24_001095"
     CDS             complement(280..873)
                     /locus_tag="JNC24_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAY9157906.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVAEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLAEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAVQYYHAAETAIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTILKE"
     gene            944..1657
                     /gene="bdcA"
                     /locus_tag="JNC24_001096"
     CDS             944..1657
                     /gene="bdcA"
                     /locus_tag="JNC24_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAY9157907.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     EHLAQKTGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMAREL
                     ARDFGPRGITINVVQPGPIDTDANPANGPMRDMLHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            1788..2183
                     /locus_tag="JNC24_001097"
     CDS             1788..2183
                     /locus_tag="JNC24_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HAY9157908.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPENQFEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEQ"
     gene            2464..2598
                     /gene="pyrL"
                     /locus_tag="JNC24_001098"
     CDS             2464..2598
                     /gene="pyrL"
                     /locus_tag="JNC24_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HAY9157909.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCPGVRR
                     "
     gene            2602..3537
                     /gene="pyrB"
                     /locus_tag="JNC24_001099"
     CDS             2602..3537
                     /gene="pyrB"
                     /locus_tag="JNC24_001099"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HAY9157910.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            3550..4011
                     /gene="pyrI"
                     /locus_tag="JNC24_001100"
     CDS             3550..4011
                     /gene="pyrI"
                     /locus_tag="JNC24_001100"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HAY9157911.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            4084..4470
                     /gene="ridA"
                     /locus_tag="JNC24_001101"
     CDS             4084..4470
                     /gene="ridA"
                     /locus_tag="JNC24_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HAY9157912.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(4676..7372)
                     /gene="mgtA"
                     /locus_tag="JNC24_001102"
     CDS             complement(4676..7372)
                     /gene="mgtA"
                     /locus_tag="JNC24_001102"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HAY9157913.1"
                     /translation="MFKEIFTRLIRHLPSRLVHRDPLPGAQQTVNTAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGENKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDIMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKMTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMIFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(7513..7566)
                     /gene="mgtL"
                     /locus_tag="JNC24_001103"
     CDS             complement(7513..7566)
                     /gene="mgtL"
                     /locus_tag="JNC24_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HAY9157914.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            7751..8698
                     /gene="treR"
                     /locus_tag="JNC24_001104"
     CDS             7751..8698
                     /gene="treR"
                     /locus_tag="JNC24_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator TreR"
                     /protein_id="HAY9157915.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMESQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            8817..10238
                     /gene="treB"
                     /locus_tag="JNC24_001105"
     CDS             8817..10238
                     /gene="treB"
                     /locus_tag="JNC24_001105"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HAY9157916.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            10288..11943
                     /gene="treC"
                     /locus_tag="JNC24_001106"
     CDS             10288..11943
                     /gene="treC"
                     /locus_tag="JNC24_001106"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HAY9157917.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVQHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DDSSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            12337..14475
                     /gene="nrdD"
                     /locus_tag="JNC24_001107"
     CDS             12337..14475
                     /gene="nrdD"
                     /locus_tag="JNC24_001107"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004128226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HAY9157918.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVSEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSRESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFGGEHVY
                     DNEQLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            14633..15097
                     /gene="nrdG"
                     /locus_tag="JNC24_001108"
     CDS             14633..15097
                     /gene="nrdG"
                     /locus_tag="JNC24_001108"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HAY9157919.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDKIIKDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVKRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(15142..15528)
                     /gene="cybC"
                     /locus_tag="JNC24_001109"
     CDS             complement(15142..15528)
                     /gene="cybC"
                     /locus_tag="JNC24_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HAY9157920.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDNLKVVEKADN
                     ATQVKDALTKMRAAALDAQKATPPKLEDKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(15712..17064)
                     /gene="pmbA"
                     /locus_tag="JNC24_001110"
     CDS             complement(15712..17064)
                     /gene="pmbA"
                     /locus_tag="JNC24_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HAY9157921.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGLADKELLAFDAPDLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLLPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            17158..17709
                     /locus_tag="JNC24_001111"
     CDS             17158..17709
                     /locus_tag="JNC24_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HAY9157922.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRAAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            complement(17792..19165)
                     /gene="mpl"
                     /locus_tag="JNC24_001112"
     CDS             complement(17792..19165)
                     /gene="mpl"
                     /locus_tag="JNC24_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HAY9157923.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLEPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVASADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            19341..20339
                     /gene="fbp"
                     /locus_tag="JNC24_001113"
     CDS             19341..20339
                     /gene="fbp"
                     /locus_tag="JNC24_001113"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HAY9157924.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENAQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEY
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            complement(20372..21367)
                     /gene="yjfF"
                     /locus_tag="JNC24_001114"
     CDS             complement(20372..21367)
                     /gene="yjfF"
                     /locus_tag="JNC24_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease YjfF"
                     /protein_id="HAY9157925.1"
                     /translation="MIKRNLPLMITIGVFVLGYLYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVIGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLVSEESIPINHPIYDTLSSLAWKIPGGG
                     RLSAMGLLMLAVVVIGIFLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLST
                     GLATLAGIVFSIYTQAGYALAGVGVELDAIASVVIGGTLLSGGVGTVLGTLFGVAIQG
                     LIQTYINFDGTLSSWWTKIAIGILLFIFIALQRGLTVLWENRQSSPVTRVNIAQQ"
     gene            complement(21354..22376)
                     /locus_tag="JNC24_001115"
     CDS             complement(21354..22376)
                     /locus_tag="JNC24_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY9157926.1"
                     /translation="MPQSLPDTTPPKRRFHWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITAGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEAVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSGVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSRDKT"
     gene            complement(22390..23892)
                     /locus_tag="JNC24_001116"
     CDS             complement(22390..23892)
                     /locus_tag="JNC24_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HAY9157927.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQAISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFFLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEVELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHVLQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDEPTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAQLSVPAIMNAIAA"
     gene            complement(24032..24988)
                     /gene="ytfQ"
                     /locus_tag="JNC24_001117"
     CDS             complement(24032..24988)
                     /gene="ytfQ"
                     /locus_tag="JNC24_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
                     /protein_id="HAY9157928.1"
                     /translation="MWKRLLVVSAVSAAMSSMALAAPLTGGFSQVGSESGWRAAETNV
                     AKSEAEKRGITLKIADGQQKQENQIKAVRSFVAQGVDAIFIAPVVATGWEPVLKEAKD
                     AEIPVFLLDRSIDVKDKSLYMTTVTADNILEGKLIGDWLVKEVNGKPCNVVELQGTVG
                     ASVAIDRKKGFAEAIKNAPNIKIIRSQSGDFTRSKGKEVMESFIKAENNGKNICMVYA
                     HNDDMVIGAIQAIKEAGLKPGKDILTGSIDGVPDIYKAMMDGEANASVELTPNMAGPA
                     FDALEKYKKDGTMPEKLTLTKSTLYLPDTAKEELEKKKNMGY"
     gene            25298..25828
                     /gene="ppa"
                     /locus_tag="JNC24_001118"
     CDS             25298..25828
                     /gene="ppa"
                     /locus_tag="JNC24_001118"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HAY9157929.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            complement(26207..26548)
                     /locus_tag="JNC24_001119"
     CDS             complement(26207..26548)
                     /locus_tag="JNC24_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HAY9157930.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            complement(26551..30330)
                     /gene="tamB"
                     /locus_tag="JNC24_001120"
     CDS             complement(26551..30330)
                     /gene="tamB"
                     /locus_tag="JNC24_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HAY9157931.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGNVTGGWRDLTLSDVRYEQPGVAVKAGNLHLAVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVAEVAQEEVVEPKIENPQPDEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGIVNASGTAQLSDNWPVDIILNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEFPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTQGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            complement(30327..32060)
                     /gene="tamA"
                     /locus_tag="JNC24_001121"
     CDS             complement(30327..32060)
                     /gene="tamA"
                     /locus_tag="JNC24_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HAY9157932.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQILDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSDWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFAGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGGGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            32266..32904
                     /gene="msrA"
                     /locus_tag="JNC24_001122"
     CDS             32266..32904
                     /gene="msrA"
                     /locus_tag="JNC24_001122"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAY9157933.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            33227..34570
                     /locus_tag="JNC24_001123"
     CDS             33227..34570
                     /locus_tag="JNC24_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAY9157934.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(34633..34839)
                     /locus_tag="JNC24_001124"
     CDS             complement(34633..34839)
                     /locus_tag="JNC24_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HAY9157935.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            35164..35718
                     /locus_tag="JNC24_001125"
     CDS             35164..35718
                     /locus_tag="JNC24_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HAY9157936.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFETGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTQEEVQQVMDLLQKLLK"
     gene            complement(35808..36719)
                     /locus_tag="JNC24_001126"
     CDS             complement(35808..36719)
                     /locus_tag="JNC24_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9157937.1"
                     /translation="MLPRIRHNNFIGAVELFVKSSYTKTHSNNFFNNIQHAFKKKDWI
                     SNYDSLLTLREFFRCATQIDKNSYQVLSSKNGTVNAMDKFLISFSLKDNGAEYTMTLR
                     GSGFEYEEIPITINEYNSFMDFKNREFPLEQNRRLYACDILQKKQSDIPKRIKGYIHQ
                     AIGDVSLGYALLEDIVSKLKRGKFELQIPGGGIKECDGWYIHEKIIDDNFAIVIESLG
                     FALKIYGGDERFRNGSSVVLEDEDYSLIYNFLVNAGCQQVELAEQVDAIVSANLAADS
                     NITKEKICKKYKSTIEAFKKEQLALPV"
BASE COUNT         8908 a         9846 c         9137 g         8873 t
ORIGIN      
        1 aaacagacga ttgccttcga tatcgtgctt gccctttggc gtttctgccg taacgtgtgc
       61 tttgatatgc tcaatcgcca ggcgtaactc ctggggtagc cacggctgaa gattatgaat
      121 atttccgatg atcatctttt ccactcctta attaaaacaa tgtttcattt ccatcgttat
      181 acgaaaagat gatcaattcc gctagcctta atgcgtgaat tattgtctta tcacaactta
      241 tcggcaggcg cgttcccgcc tttcgggtca aggcccgcat cactccttga gaatagtttt
      301 tatcgcttcc cccgccagtg cagctgttgc acagagttgt tcgatggagt gcccctcccg
      361 agccttcgca gaaagccctg acatcacggt actcataaaa tcagtcacac attgtgcgct
      421 ttgtggatgc cgcctggcga tatagtcata aatggccgtt tctgcggcgt gataatattg
      481 aacggcaata tcacgcgctt gtggatcatg actatgaatg ccttcaagaa ccatacagcc
      541 agcgcagccg ccgttttggc tatatctgcg cgccgcttct tttaatacct cagccaggca
      601 ctcccctacc ggacgatcat cacgaagaat atcggcaagc ggaatagctt ccgtaccgac
      661 atattcattg agtacacggc taaataaccc agccttactg ccaaaagccg cgtagaggct
      721 cggggggtta ataccaagat aatcagtaac ttcagcaaca ctgacagcat cgaaaccttt
      781 ttggtgaaaa agcacttttg ccgcagagac tgcctgctca ggagcgaaac gtctgggacg
      841 acctggaacg cgagattgtt ttttagtgac cattacaaaa cttgttgaca gaaagttaaa
      901 acagttttgt aatgcatgtt acataataaa tcaaggagtc cttatgggcg cttttacagg
      961 taagacagtt ctcatcctcg gtggcagtcg tggtatcggt gccgctatcg tacgtcgttt
     1021 cgtcaccgat ggggccaatg tacgattcac ctatgcgggg tcgaaagatg ccgctgaaca
     1081 cctggcacaa aagactggag cgacagcagt attcacagat agtgctgaca gagacgctgt
     1141 cattgatgtc gttcgtaaga gcggcgcatt ggatatcctg gtggtaaatg caggtattgg
     1201 cgtctttggc gatgccctgg aattgaatgc cgacgatatt gatcgccttt tcaaaatcaa
     1261 tattcatgct ccttatcatg cctctgttga agccgcccgg cagatgcccg aaggcgggcg
     1321 catcttaatc atcggctccg tgaatggcga tcgtatgcct gttgcaggca tggctgctta
     1381 tgccgccagt aaatctgccc tgcaaggcat ggcgcgcgag ctggcccgtg atttcggacc
     1441 gcgtgggatc accatcaacg tcgtccagcc agggccaatt gataccgacg ctaatcccgc
     1501 caacgggcca atgcgcgata tgttgcatag ttttatggct atcaaaagac atgggcaacc
     1561 ggaagaggtc gctggtatgg tcgcatggtt agcagggcca gaagccagtt ttgttaccgg
     1621 cgcgatgcat accattgatg gcgcgtttgg cgcataaccg actacgctca attaagccca
     1681 gccatttccc atgatgtctg ggttttgttt actcacgtcg tccgctaaaa gcggctcctg
     1741 gtaaatataa atcttctgtt catgtcgtaa atcttgagga taaccaaatg gtagaaagaa
     1801 ccgctgtttt ccctgcaggc cgacattcac tgtatgctga acatcgttat tctgcggcta
     1861 ttcgttccgg cgatttgctg tttgtttccg ggcaagtagg aagtcgagag gacggaacac
     1921 cagaacccga ttttcagcaa caagtcagac tggcatttga taatttgcat gcgaccctgg
     1981 ctgctgcggg atgcactttt gacgatatca ttgatgttac gagcttccat accgatccag
     2041 aaaaccaatt tgaagacatc atgacggtga aaaatgaaat atttagcgcc ccaccttatc
     2101 caaactggac ggcggtaggt gttacatggc tggcaggctt tgattttgaa attaaagtga
     2161 tagcgcgcat ccctgagcag taagcaatag tgttagccgt tcgttttcac actccgccct
     2221 ataagtcgga tgaatggaat aaaatgcata tctgattgcg tgaaagtgaa aaaggaaaaa
     2281 gcaggaaatg tctgcaatta ttgataccga acgaccgttc cccagcagaa tcacaacaaa
     2341 taaaaatgca tgtattttga cttataattc aaataaaccg tttgcgctga caaaatattg
     2401 catcaaatgc ttgcaccgct tctgacgatg agtataatgc cggacaattt gccgggagga
     2461 tgtatggttc agtgtgttcg acattttgtc ttaccgcgtc tgaaaaaaga cgctggcctg
     2521 ccgtttttct tcccgttgat cacccattcc cagcccctca atcgaggggc ttttttttgc
     2581 ccaggcgtca ggagataaaa gatggctaat ccgctatatc agaaacatat catttccata
     2641 aacgacctta gtcgcgatga ccttaatctg gtgctggcga cagcggcgaa actgaaagca
     2701 aacccgcaac cagagctgtt gaagcacaaa gtcattgcca gctgtttctt cgaagcctct
     2761 acccgtaccc gcctctcttt cgaaacttcc atgcaccgcc tgggtgccag cgtggtgggc
     2821 ttctccgaca gcgccaatac atcactgggt aaaaagggcg aaacgctggc cgataccatt
     2881 tcggttatca gcacttacgt cgatgcgata gtgatgcgtc atccgcagga aggtgcggcg
     2941 cgcctggcca ccgagttttc cggcaatgta ccggtactga atgccggtga tggctccaac
     3001 caacatccga cgcaaacctt gctggactta ttcactattc aggaaaccca ggggcgtctg
     3061 gacaatctcc acgtcgcaat ggttggtgac ctgaaatatg gccgcaccgt tcactccctg
     3121 actcaggcgt tagcgaagtt cgacggcaac cgtttttact tcatcgcgcc ggacgcgctg
     3181 gcaatgccgc aatacattct ggatatgctt gatgaaaaag ggatcgcctg gagtctgcac
     3241 agctctattg aagaagtgat ggcggaagta gacatcctgt acatgacccg cgtgcaaaaa
     3301 gagcgtctgg acccgtccga gtacgccaac gtgaaagcgc agtttgttct tcgcgccagc
     3361 gatctccaca acgccaaagc caatatgaaa gtgctgcatc cgctgccgcg tgttgatgag
     3421 attgcgacgg atgttgataa aacgccacac gcctggtact tccagcaggc aggcaacggg
     3481 attttcgctc gccaggcgtt actggcactg gttctgaatc gcgatctggt actgtaaggg
     3541 gaaatagaga tgacacacga taataaattg caggttgaag ctattaaacg cggcacggta
     3601 attgaccata tccccgccca gatcggtttt aagctgttga gtctgttcaa gctgaccgaa
     3661 acggatcagc gcatcaccat tggtctgaac ctgccttctg gcgagatggg ccgcaaagat
     3721 ctgatcaaaa tcgaaaatac ctttttgagt gaagatcagg tagatcaact ggcattgtat
     3781 gcgccgcaag ccacggttaa ccgtatcgac aactatgaag tggtgggtaa atcacgcccc
     3841 agtctgccgg agcgcatcga caatgtgctg gtctgcccga acagcaactg tatcagccat
     3901 gccgaaccgg tttcttccag ctttgccgtg cgaaaacgcg ccaatgacat cgcgctcaaa
     3961 tgcaaatact gtgaaaaaga gttttcccat aatgtggtgc tggccaatta attgcggttg
     4021 gtaataaaag tctggctccc tataatgagc cagacttttt accgctgtaa taaaggagaa
     4081 atcatgagca aaactatcgc gacggaaaat gcaccggcag ctatcggtcc ttacgtacag
     4141 ggcgttgatc tgggcaatat gatcatcacc tccggtcaga tcccggtaaa tccgaaaacg
     4201 ggcgaagtac cggcagacgt cgctgcacag gcacgtcagt cgctggataa cgtaaaagcg
     4261 atcgtcgaag ccgctggcct gaaagtgggc gacatcgtta aaaccaccgt gtttgtgaaa
     4321 gatctgaacg acttcgcaac cgttaatgct acctacgaag ccttcttcac cgaacacaat
     4381 gccaccttcc cggcacgttc ttgcgttgaa gttgcccgtc tgccgaaaga cgtgaaaatt
     4441 gagatcgaag cgatcgctgt tcgtcgctaa tcttgatgga aatccgggct atcatgcccg
     4501 gattaagtct gatgacaaac acaaaatcgc ctgatgcgct acgcttatca ggcctacgtg
     4561 attcctgcaa tttattgaat ttgttggccg gataaggcat ttacgccgca tccggcatga
     4621 acaaaactca ctttgtctgc aatctgaatc caggccacca tgcccagttg attctttatt
     4681 gccagccgta acgacggcta taaaatcctt tcaccagttg ggttaacgtc atataccctg
     4741 ccagaatcgc aaccagccac gggaaatagc ttaacggcag cgcctgcagt tgcagataac
     4801 tggccagcgg tgaaaacggc aatgcgatcc cgacaatcat cacgatcacg gtcatgatca
     4861 ttaacggcca cgatgcacag ctctgaataa acggcacacg gcgggtgcgg atcatatgca
     4921 caatcagcgt ttgcgacagc aagcccacca caaaccatcc cgactggaac agcgtttgcg
     4981 tttccggcgt gttggcatgg aatacccacc acatcaggca aaacgtcaga atatcgaaga
     5041 tcgagctgat cggtccgaag aagatcataa agcgccccag atccgccgga ttccaacgct
     5101 gcggcttttg aatttgctcg tcgtcgacgt tatcaaacgg gatcgccacc tgtgacacat
     5161 catacagcag gttctgaata agcaagtgta acggcaacat cggcaggaag ggcaagaaag
     5221 cactcgccac cagcacgctg aacacattac cgaagttgga gctcgccgtc attttgatgt
     5281 atttcagcat gttggcgaaa gtgcgacgtc cttcaataac cccctcttcc agcaccatca
     5341 ggcttttttc cagcaggatg atatcagccg cttcacgggc aatatctacc gcgccgtcca
     5401 cagaaatgcc gatatccgca gcgcgtaagg cgggcgcatc attaatacca tcgcccataa
     5461 agccaaccac atgcccttcg cgcttcagta aggtcacaat gcgttcttta tgcatcggcg
     5521 tcagacgagc aaacagcgtg gtgcgctgtg cgagatttgc cagttcgtcg tcagatagcg
     5581 tttcaatatc actaccaatg accacctctc ccgcgtccag ccccacttca tggcacactt
     5641 tcgctgcaac taactcactg tcgccagtaa ggatttttac ggtaattcca ctcgctttta
     5701 atgccttcag tgccggagca gttgtctctt ttggcggatc aaggaaagca atatatcctt
     5761 cgaggatcag gtcggattca tccgcccgct ggtaatctcc ttcacgcgct ggcaggtatt
     5821 tcgtcgccac cgcaaccacg cgcagcccct gacgattcag cgtatcagta acccgcttaa
     5881 tcttacgcag catgatgtca tcgagcggca caatctcgcc attgtgacgc acctgcgaac
     5941 acacattgag gatttcctgc aatgcacctt tgcaaaccag ctgatggtgc tcggtatttt
     6001 ctgccactac caccgacatc cggcgacgct cgaaatcaaa cggaatctca tcaatttttt
     6061 gccaacggct ggccagagag cgcgctgact cttcatccgt accttcgagc accgctgtat
     6121 cgagtaggtt tttaagtccg gtctgataat gactgttcaa ccacgcgcta tgcagcacgc
     6181 gttcgctggt tttaccggag atatcggtat gattctccag cacaattttg tcctgtgtca
     6241 gggtgccagt tttatcagtg cacagaatat ccattgcgcc aaagttctga atagcatcca
     6301 gatgtttgac gatcactttc tgtttcgaca gctttactgc gccgcgcgcc agcgtcgagg
     6361 taacaatcat cggcaacatt tccggcgtta agcctaccgc gaccgaaagc gcaaacagtg
     6421 ccgcttccca ccagtcgcct ttggtgtaac cgttgataag caacaccacc ggagccatca
     6481 ccagcataaa gcgaatcagc agcatactga cgcggctgat cccctgctga aaggcattcg
     6541 gctcgctttc ctgctcacta acacgccccg ccagttgacc aaaccaggtg ttggctcctg
     6601 tagcaatcac cattgcttgt gccgtgccgc tcaccacggt ggtgcccata aaacacaggg
     6661 tgtcacactc cagcggattg ctgtgctccg gctggcgagt ggtagcggct ttttccacgg
     6721 gcagagactc accggttaac gacgcctgag cgacgaacag atcccgcgcc tgcaagatac
     6781 gtaaatctgc cgggatcata tcccccgccg ccagttttat aatatcgccg ggcaccagct
     6841 ggtcgatcgg gatctccagc cagccatttt cgcctttgtc gttaattacg cgcagcaccg
     6901 tcgcggtatt gctgaccatc gctttcaggg catctgccgc tttagtggaa cgtgcttcct
     6961 gaataaagtt cagcaacgta gaaatagcga ccattagcgc gataacgccc gcggcaaata
     7021 aatcttcagt ggcgtaagaa atagcgccga gaatggtgag taaaatatta aatgggttgc
     7081 gatagcagac ccataaatgt acccaccacg gcgacggttg ttgtgcgggt aatttatttt
     7141 caccatgttg ttcgcgggca gattccactt ccgcctgatt taacccttcc ggatgggtgt
     7201 cgaacgtttt ccacaattct tcttcgggca tcaccgccat tttcaggcaa tgcgcactta
     7261 aggacggcgg gaccgccgta ttcactgtct gctgcgcgcc aggcaatgga tcgcgatgaa
     7321 ccagacggga aggtaaatgg cgaatgagcc gggtaaaaat ttctttaaac ataaggagtc
     7381 cctccgcact gtctgaaaca gtacagaaat gtcttcaggc gtggcgaagc cttacctgaa
     7441 ggtaataaag ctgagcaggg acgttaaaac gtcacggcct tacgcatccg gtagcgccgt
     7501 gaaaagacgg gcttaccgga aaagttttaa tctccgtcga gggagaggcg tgggatcagg
     7561 ttccatataa cctccggtaa gtgaaaaaag cgccgcaaat aataaggaga atccaataat
     7621 attcgtagca tttatggcga tataatactt gctggcaact aaaccaacga taaaccagac
     7681 tttacccatt gctgaatgca cgggtaaagt taggctcaaa taattaaaca acacgttaca
     7741 ggacaacagg atgcaaaatc ggctgactat caaagacatc gcacgcttaa gcggcgtggg
     7801 gaaatctaca gtttcccggg tgctgaataa cgaaagcggc gtgagccagc gcacccgcga
     7861 gcgtgttgaa gcagtgatga atcagcatgg attttcccct tcccgctctg cgcgcgctat
     7921 gcgtgggcaa agcgataaag tggtcgccat cattgttacc cgtctggatt cgttgtcaga
     7981 aaatctcgcc gttcaaacca tgctgccagc gttctatgaa caaggttacg acccaatcat
     8041 gatggaaagc cagttttccc cgcaattagt tgccgaacat ttgggggtgc tgaaacggcg
     8101 taatatcgac ggcgtagtgc tgttcggttt tactggcata acagaagaaa tgttagccca
     8161 ctggcagtca tcgctggttc tgctggcgcg tgacgcaaaa ggctttgctt cggtctgtta
     8221 tgacgacgaa ggggcaatca agatcctgat gcaacggctg tatgaccagg ggcatcgtaa
     8281 tatcagttat ctcggcgtgc cgcatagtga cgtgacaacc ggtaagcgac gtcacgaagc
     8341 ctacctggcg ttctgcaaag cgcataaact gcatcccgtt gccgctctgc cagggcttgc
     8401 tatgaagcaa ggctatgaga acgtggcaaa agtgattacg cctgaaacta ctgcgttact
     8461 gtgcgcaacc gacacgctgg cacttggcgc aagtaaatac ctgcaagagc aacgcatcga
     8521 caccttgcaa ctggcgagcg tcggtaatac accgttaatg aaattcctcc atccggagat
     8581 cgtcaccgta gatcccggtt acgccgaagc tggacgccag gcggcctgcc aattgatcgc
     8641 acaggtaacc gggcgcagcg aaccgcaaca aatcatcatc cccgccaccc tgtcctgatc
     8701 gtttcctgaa cgataaattg tgatcttcgc tgcatttcgg gaacgttccc gtttttaaat
     8761 ttttccgcgc aatatattct gcagccaacc aaaaatgtca tctgccatgg ggctttatga
     8821 tgagcaaaat aaaccaaacg gatatcgatc ggttgattga actggtcggc gggcgcggca
     8881 atattgcgac ggtgagccac tgtattactc gcctgcgctt tgtcctcaac caaccggcca
     8941 atgccagacc gaaagaaatt gagcaactcc ccatggtgaa aggctgtttc accaatgccg
     9001 ggcaatttca ggtggtgatt ggcaccaacg tgggtgatta ctatcaagca ctaatagcgt
     9061 caaccggaca ggcgcaggtt gataaagagc aggtaaaaaa agccgcccgg cagaatatga
     9121 aatggcatga gcagttgatc tctcatttcg cggagatctt cttcccgttg ctgcccgcgt
     9181 tgattagcgg cggtttgatc ctcggttttc gcaatgtgat cggcgatttg cccatgagca
     9241 acggtcagac gctggcgcaa atgtaccctt ccctgcaaac gatctacgat tttctgtggt
     9301 tgatcggtga agcgattttc ttctacctgc cggtcgggat ttgctggtca gcggtgaaaa
     9361 aaatgggcgg cacgccgatc cttggtatcg tgcttggcgt gacactggtt tccccccagc
     9421 tgatgaacgc ttatctgctc gggcagcagc tgccggaagt gtgggacttt ggcatgttca
     9481 gcatcgccaa agtgggctat caggcgcagg tgatcccggc actgttagcc gggctggcgc
     9541 tgggcgttat tgaaactcgc cttaaacgca tcgtaccgga ttacctctat ctggtggtgg
     9601 tgcccgtctg ttcgctgatc ctcgcggtgt tcctcgccca tgcgctgatt ggtccgtttg
     9661 gtcgcatgat tggcgatggc gttgcctttg cggtacgtca cctgatgacc ggcagctttg
     9721 ctccgattgg tgcggcattg tttggcttcc tgtacgcgcc gctggtgatc accggcgtac
     9781 accagaccac ccttgctatt gatttgcaga tgattcaaag catgggtggc acgccagtgt
     9841 ggccgctgat tgcgctgtcg aatatcgctc agggctccgc cgtgataggc attatcattt
     9901 ccagccgcaa gcacaatgaa cgcgagatct ccgtgcctgc cgctatctcc gcctggcttg
     9961 gggtcactga gcctgcaatg tacggcatca acctgaaata tcgcttcccg atgctgtgcg
    10021 cgatgattgg ttctggtctg gcaggattgc tatgcggcct gaacggcgtt atggcgaatg
    10081 gtatcggcgt agggggcctg ccgggaattc tctcgattca accgagctac tggcaggtat
    10141 ttgcgctggc aatggctatc gccatcatca tcccgattgt actcacctcg tttatctatc
    10201 agcggaaata ccgcttgggc acgctggaca ttgtttaatt ttcttcgggg cgcaattgcg
    10261 ccccctcgca tttgcaggaa taacgtaatg actaatcttc cccactggtg gcaaaacggc
    10321 gttatctacc agatttatcc aaagagtttt caggacacca cgggtagcgg caccggcgat
    10381 ttacgtggcg ttatccaacg cctggactat ctgcataaac tgggcgttga tgccatctgg
    10441 ctaaccccct tttatgtctc tccccaggtc gataacggtt acgatgtagc gaactatacg
    10501 gcgattgatc ccacctacgg cacgctggac gattttgacg aactggtgac gcaggcaaaa
    10561 tcgcgcggga ttcgtatcat tctcgatatg gtgtttaacc atacctctac ccaacatgcc
    10621 tggtttcgcg aggcgctgaa caaagaaagc ccttaccgcc agttttatat ctggcgcgat
    10681 ggcgaaccag aaacgccacc gaacaactgg cgttcaaaat ttggcggtag tgcgtggcgc
    10741 tggcatgcgg aaagcgaaca gtactatttg catctctttg caccagaaca ggcggatctc
    10801 aactgggaga atccagcggt acgcgcagag ctgaaaaaag tctgtgaatt ctgggccgat
    10861 cgtggggtcg acgggttgcg cctggatgtg gtgaatctga tctccaaaga cccgcgtttc
    10921 cctgaagacc tggatggcga cgggcgtcgc ttctacaccg acgggccacg agcacacgag
    10981 tttttgcacg agatgaaccg cgatgtgttt acgccacgcg ggttaatgac cgtaggtgaa
    11041 atgtcctcca ccagccttga gcattgccag cgatacgcgg cactgacagg cagtgaattg
    11101 tcgatgacct ttaattttca tcacctgaag gtcgattatc ccggtggtga aaaatggacg
    11161 ctggctaaac ctgactttgt ggcgttgaaa acattgttcc gccactggca acaaggaatg
    11221 cacaacgtgg catggaatgc cttgttctgg tgtaaccacg atcagccgcg cattgtttct
    11281 cgctttggtg atgaaggtga ataccgcgtg cctgcggcaa aaatgctggc gatggtgcag
    11341 catggcatgc agggaacgcc gtatatctac cagggcgaag agattggcat gaccaacccg
    11401 catttcacgc gcattactga ctatcgcgac gtggagagcc tcaatatgtt tgccgagctg
    11461 cgcaacgatg gtcgtgatgc cgacgagtta ttggcaatcc tcgccagtaa atcccgtgac
    11521 aacagccgca cgcccatgca atggagcaac ggcgataatg ccgggtttac ggctggcgaa
    11581 ccgtggattg gcctgggcga taactatcaa caaatcaacg tagaagccgc gctggccgat
    11641 gactcctcgg tgttttacac ctaccaaaag ttaatcgcac tgcgtaagca agaagccatc
    11701 ctgacatggg gcaattacca ggatctgctg ccaaacagcc ctgtattgtg gtgctatcgc
    11761 cgtgaatgga aggggcaaac cttgctggtc attgccaacc ttagccgtga gatccaaccc
    11821 tggcagccag ggcaaatgcg cggcaactgg cagcttgtga tgcataacta cgaagaagcc
    11881 tcaccacaac cctgtgccat gaatttacgg ccttttgagg ctgtctggtg gttacagaag
    11941 taaatcttcc ctaagccccg gtaatgccgg tcattccggg gttttgtctg tcagcatgtt
    12001 gtttttgttg atttttcaac cagcaaattc attaaaaaat ttacataacg ctgtagcgcc
    12061 cgtcatccgt acgctctgct ttttactttg agctacatca aaaaaagtta aaacatcctt
    12121 gatgcaaagc actatatata gactttaaaa tgcgtcccaa cccaatatgt tgtattaatc
    12181 gactataatt gctactacag ctccccacga aaaaggtgcg gcgttgtgga taagcggatg
    12241 gcgattgcgg aaagcaccgg aaaacgaaac gaaaaaaccg gaaaacgcct ttcccaattt
    12301 ctgtggataa cctgttctta aaaatatgga gcgatcatga caccgcatgt gatgaaacga
    12361 gacggctgca aagtgccgtt taaatcagag cgcatcaaag aagcgattct gcgtgcagct
    12421 aaagcagcgg aagtcgatga tgccgattat tgcgccactg ttgccgcggt tgtcagcgag
    12481 cagatgcagg gccgcaacca ggtggatatc aacgagatcc agaccgcagt tgaaaaccag
    12541 ctgatgtcgg gtccatacaa acaactggct cgtgcttaca tcgagtaccg tcacgatcgc
    12601 gacattgaac gtgaaaaacg cggtcgcctg aaccaggaga tccgtggtct ggtcgagcag
    12661 accaacgcct cgttactcaa cgaaaacgcc aacaaagaca gcaaggtgat tccaacccag
    12721 cgcgacctgc tggccgggat cgtggctaaa cactatgcac gccagcacct gctgccgcgt
    12781 gacgtggtgc aggcacatga gcgtggcgat attcactatc acgatctcga ttactcgccg
    12841 ttcttcccga tgttcaactg catgttgatc gacctgaaag gcatgctgac ccagggcttt
    12901 aaaatgggga acgccgagat tgaaccgccg aagtcgatct ctacggcaac cgcggtaact
    12961 gcgcagatta ttgctcaggt cgccagccat atttatggcg gcaccaccat taaccgtatc
    13021 gatgaagtgc tggcgccgtt tgttaccgcc agctacaaca aacatcgcaa gaccgcagaa
    13081 gagtggaaca tcccggacgc cgaaggctac gctaactctc gtaccatcaa agagtgctac
    13141 gatgccttcc agtcgctgga gtacgaagta aacaccctgc acaccgccaa cggtcagacg
    13201 ccgtttgtaa cctttggttt tggtctgggc accagcaggg aatcgcgcct gattcaggaa
    13261 tcgatcctgc gtaaccgtat cgcaggcctc ggtaaaaacc gtaaaactgc ggtgttcccg
    13321 aaactggtgt ttgcgattcg cgatggcctg aaccataaaa aaggcgatcc gaactacgac
    13381 atcaaacagc tggcgctgga gtgcgcaagc aagcgcatgt atccggatat cctgaactac
    13441 gatcaggtag tgaaagtcac cggttcgttt aaaaccccga tgggctgccg cagcttcctc
    13501 ggcgtgtggg aaaatgaaaa cggcgagcag atccacgatg gtcgtaacaa cctcggcgtg
    13561 atcagcctga acctgccgcg tattgctctg gaagcaaaag gcgatgaagc caccttctgg
    13621 aagctgctgg atgaacgtct ggtgctggca cgtaaggcac tgatgacccg tatcgctcgt
    13681 ctcgaaggcg tgaaagcgcg cgtcgccccg atcctctata tggaaggtgc ttgtggcgta
    13741 cgtctgaatg ctgacgatga tgtttctgaa atcttcaaaa acggtcgtgc gtctatttcg
    13801 ctgggttaca tcggcatcca cgaaaccatt aacgcgctgt tcggcggcga gcatgtttac
    13861 gacaacgagc agcttcgcgc gaaaggtatc gccattgttg aacgtctgcg tcaggcagtg
    13921 gatcagtgga aagaagaaac gggttatggt ttcagtctct acagcacgcc gagtgaaaac
    13981 ctgtgcgacc gcttctgccg tctcgatact gctgagtttg gcgtggtgcc gggcgtgacc
    14041 gataaaggtt actacaccaa cagtttccac cttgatgtgg agaagaaggt gaacccgtac
    14101 gacaagatcg actttgaagc gccttacccg ccgctggcga acggtggttt catttgctac
    14161 ggcgagtatc caaacattca gcacaacctg aaagcgctgg aagatgtctg ggattacagc
    14221 tatcagcatg taccgtatta cggcaccaat acaccgattg atgagtgcta cgagtgtggc
    14281 tttaccggtg agttcgagtg caccagcaaa ggcttcactt gcccgaaatg tggtaaccat
    14341 gacgcctccc gtgtgtcggt aactcgccgc gtgtgcggat atttaggtag cccggatgcg
    14401 cgtccgttta acgccggtaa gcaggaagaa gttaagcgcc gcgttaaaca tttggggaat
    14461 gggcagatag gttaatcttc actctgcgtt aagcaaatgc cggatgcggc gtaaacgcct
    14521 tatccggcct acatttggca acggctgtag gcctgataag acgcgacagc gtcgcatcag
    14581 gcattgatta ccggatgcag cctacacgcg gactgataag ccaggcgaaa taatgaatta
    14641 tcatcagtac tatcctgtcg acatcgtcaa cggccccggc actcgctgca ccctgtttgt
    14701 ctccgggtgt gttcatgaat gccccggttg ctataacaaa agcacatggc gggtaaattc
    14761 tggtcagcca tttaccaaag caatggaaga caagatcatt aaagatctga atgacactcg
    14821 tatcaaacgc caggggattt ccctctccgg cggcgatccg ctgcatccgc aaaacgtgcc
    14881 ggatattctg aaactggtaa aacgcatccg cgccgagtgt ccgggtaaag acatctgggt
    14941 gtggacaggc tataaactcg acgaactcaa cgctgcgcaa atgcaggttg ttgatttgat
    15001 taacgtgctg gtcgacggca aatttgtgca ggatttaaaa gacccttccc tgatctggcg
    15061 cggcagcagc aatcaggtgg tgcatcattt gcgatgataa gttccctgtt acctgagtgc
    15121 aggcaacagg gaaatgagga attaacgata cttctggtga taggcgttgc gggtcgtttt
    15181 cagttgctct gcagcagcct gcgcttcttt tactttacct tcatttgcca gcttcagcgc
    15241 gtcgtcaatc tgaccgacca gaatgtcgaa accgtggcgg aaatctttca tttccgggct
    15301 gtccggtgat ttatcttcga gcttcggcgg cgttgctttt tgcgcatcca gcgctgcggc
    15361 gcgcatcttc gttaacgcgt ctttgacttg cgtcgcgtta tcggcttttt cgaccacttt
    15421 taaattgtcg ttgagggttt ccatattgtc ttcaagatca gcggcaaacg acgccgaact
    15481 gaataccaac gaggagactg caagaatagc taacaggctt ttacgcattg ctcacttcct
    15541 tttttattta cacttcatcc ttcacactgc ctcatcgaca gctacgctca ttcgaccggg
    15601 tcacgtacta cgagtacgct ccccggctct cattcacttt ccgtctcgat gcagtgcgaa
    15661 tgattttgtg taatttttta gtttatagcg cggcaggtcg cgccagtttt tttactgtcc
    15721 ggcgattttc atctccggca acagcacaga accacactgt atattactgc gtgtttcaat
    15781 atcgttaccg acggtgacaa tattgcgcca catatctttt aaattacctg cgatggtgat
    15841 ttcgctcacc ggatactgaa tttcgccatt ctctacccag aagcccgcag caccacggga
    15901 ataatcaccg gtaatggcac tcacgccctg gcccatcagc tccgtcacca ccagcccggt
    15961 gcccatctct ttgagcattt gttcgaagct tagcccttgt ccggcaatcc gccagttgtg
    16021 aataccgccc gcatgtccgg tgcttttcag cccaagtttt cgcgccgaat agctggtcag
    16081 cagccactga gtcaggatgc catctttaat aatatcgcga cgctcggtgc gcacaccttc
    16141 gctgtcgaat ggcgtcgacg ccagcccttt cagcagatgc ggatgctctt caatggtcag
    16201 ccagtccggc agaatttgtt tacccagcga atccagcagg aaggtagatt tacgataaac
    16261 cgatccaccc gctatcgccc ccaccagatg cccaaaaagc ccagttgcca cttcattggc
    16321 aaaaatgact ggcgctttca tggtggagag tttacgcggt aacaggcgcg ataaagtacg
    16381 gcgagcacag tcggccccaa cccactctgg cgtttgcaga tcgctcatcg cgcgcccaat
    16441 ggtgtaggcg taatcacgct ccatatcgcc attttcttcg gcaattacac agctggagag
    16501 cgaatgacgc gttgagcagt aaccctgcaa catgccgtgg ctgttgccaa aaactttgac
    16561 accgtagtgg ctgttaaagc tgccaccttc ggtattggtg atacgtttat ccgcctgtaa
    16621 tgctgcctgt tctgcgcggg ccgccagttc aatggcttca tccggggaaa cttccgcagg
    16681 gtggaacaag tcgagatctg gtgcgtcaaa ggccagcagc tctttgtctg ccaggccggc
    16741 acagggatct ggcgaggtat aacgggcaat atccagcgcc gcctgtacag tacgggcaat
    16801 ggcctgcggg cttaaatcgg tggatgatgc gctacctttg cggttctggt gataaacagt
    16861 gatccccagc gcgccatcgc tattgaattc aacattctcc acttcaccat aacgcgtgct
    16921 tacgctaatg ccggtggtct tgctgacggc aacttccgca ccgtccgatt tgcctgaggc
    16981 caactccagc gcagtggaaa ctgcttcttc cagaatcttg cgctgcgctt caacttgaga
    17041 gattactttc attgcaagtg ccataatgta gagagtttct ctgaagtcta acagagaacc
    17101 gtttttcagt gcgcatctta actggtaaca ttagcctctt tttttaagga gcctgagatg
    17161 actaagcagc ccgaagactg gctcgacgac gttcccggtg atgacatcga agacgaagac
    17221 gatgaaatta tctgggtcag taaaagtgaa attaaacgtg atgccgagga gctaaaacgc
    17281 cttggcgcgg aaattgttga tctggggaaa aacgcgctgg ataagatccc gttagatgcg
    17341 gatctgcgcg cggctattga gctcgcccag cgtattaaga tggaaggtcg ccgccgccag
    17401 ttgcagctca tcggtaagat gctgcgccag cgcgacgtag agcctattcg tcaggcgctg
    17461 gataagctga aaaaccgtca caaccagcag gtggtgctgt tccataaact ggaaaacctg
    17521 cgcgatcgtc tgatcgatca aggtgatgac gccatcgctg aagtattgaa tctgtggccc
    17581 gatgccgatc gtcagcaact gcgtacactg atccgcaacg cgaagaaaga gaaagaaggg
    17641 aataagccgc cgaaatccgc acgccagatt ttccagtatc tgcgcgaact ggcggagaat
    17701 gaagggtaat tttcgttgtt gatgtgagta tggtttgccg gatgcggcgt aaacgcctta
    17761 tccggcaatg ctatatcagg ctgaagccga attactgcgc agcttccgcc ttcttcgcca
    17821 gaccatccag cagtttctga tggatcccac caaaaccgcc gttgctcatc accagaatat
    17881 ggtcgccagg ctgagcggtt ttcaccacca tatctgccag cgtatccaca tcgccactcc
    17941 agtgtgcagg ctgaacgcag gcttctgcca cttctgccac ctgccacgga atatgcgccg
    18001 gttgcagcag gaagacttca tcggcacgac ctaatgaagg tgccagatcg tctttgcaga
    18061 tccccatttt catggtattc gagcgcggtt ccagcacagc aataatgcgc gccgtaccac
    18121 caactttgcc acgcagcgcc gccagcgttg ccagaatcgc cgtcgggtga tgggcaaaat
    18181 cgtcatatac cgtgacgcca ttcgcttcac cacgcaactc cagacggcga cgagcattaa
    18241 taaacgaacc cagcgcgtta gcggcatctg ccgacgctac accaacatgg cgagccgctg
    18301 caatcgccat caggccattg tgcatattat gttcgcctac cagcgaccat ttcacttcgc
    18361 ccactttttc gccatccagc aaaacttccc attcggaagc atcggtggtc agctttttcg
    18421 cctgccagtg accctgctca cccaccagct cctgctcgct ccagcagccc atcgccatgg
    18481 tctgtttcag gttgatgtca ttttccggcc agataatacg gccctgcccc ggaacgatac
    18541 gcaccagatg gtggaactgt ttctggatcg ctttcaggtc gtcaaagata tcggcgtgat
    18601 cgaactcaag gttgttgagg atcagcgtac gcgggcagta atggacaaat ttagagcgtt
    18661 tgtcgaagaa ggcgcagtcg tactcatccg cttcgataac aaagaagtcg ctttcgccca
    18721 gacgcgccga aacctcaaag ttccccggca caccgccgat cacaaatccc ggtttgtaac
    18781 cacactgttc cagaatccag gtcgccattc ccgcggtggt ggttttgccg tgtgtaccgg
    18841 caacggccag cacccagcgg tcgcgcagca caaaatcgtg cagccactgt ggacctgaca
    18901 tataagggat gtttttttcc agtaccgctt ccacacacgg atttccacgg gtcatggcgt
    18961 tgccaataat caccagatcc ggctgcggct cgagctggct ggcatcgtaa ccctgaatca
    19021 gctcaatgcc ttgcttctca agtaaggtgc tcatcggcgg atacacattg gcgtccgaac
    19081 ccgttacttc atggcctaac tggcgcgcca gcatcgccag accgcccata aatgtgccac
    19141 aaattcctaa aatatgaatg cgcatacgtc actatccttt tttaatctgc cgctcatttt
    19201 acgcatatgt cgggcaagtg agaaacgcat ttcaggataa tccgcaattt gctggcgcga
    19261 ttcacctgag cgcaacattg agattatttg ttaagattgt tgcggtcgct ttactccata
    19321 aacattgcag ggaaagtttt atgaaaacgt taggtgaatt tattgtcgaa aagcagcacg
    19381 agttttctca tgctaccggt gagctcactg ctttgctgtc ggcaataaaa ctgggcgcca
    19441 agattatcca tcgcgatatc aacaaagcag gactggttga tatcctgggt gccagcggtg
    19501 ctgagaacgc gcagggcgag gttcagcaaa aactcgactt gttcgctaat gaaaaactga
    19561 aagccgcact gaaagcacgc gatatcgttg cggggattgc ctctgaagaa gaagatgaga
    19621 ttgtcgtctt tgaaggctgt gaatacgcaa aatacgtggt gctgatggac cccctggatg
    19681 gctcgtccaa catcgatgtt aacgtctctg tcggtaccat tttctccatc taccgccgcg
    19741 ttacgcctgt tggcacgccg gtaacggaag aagatttcct ccagcctggt aacaaacagg
    19801 ttgcggcagg ttacgtggta tacggctcct ctaccatgct ggtttacacc accggatgcg
    19861 gtgttcacgc ctttacttac gatccttcgc tgggcgtttt ctgcctgtgc caggaacgga
    19921 tgcgcttccc ggagaaaggc aaaacctact ccatcaacga aggaaactac attaagtttc
    19981 cgaacggggt gaagaagtac attaaattct gccaggaaga agataaatcc accaatcgcc
    20041 cgtatacctc gcgttatatc ggttcactgg ttgcggattt ccaccgtaac ctgctgaaag
    20101 gcggtattta tctctaccca agcaccgcca gccacccgga cggcaaactg cgtttgcttt
    20161 atgagtgcaa cccgatggca ttcctggcgg aacaagcggg cggtaaagcg agcgatggca
    20221 aagagcgtat tctggatatc atcccggaaa ccctgcacca gcgccgttcg ttctttgtcg
    20281 gcaacgacca tatggttgaa gatgtcgaac gctttatccg tgagttcccg gacgcgtaat
    20341 ctcctgcgcc tctccacacg gagaggcgtt tttattgctg cgcaatgttg actcttgtca
    20401 ctggcgaact ctgacgattc tcccacagca ccgttaatcc acgctgtaat gctataaaaa
    20461 taaacaacaa aataccgatg gcgattttcg tccaccagga actcagcgtg ccatcaaagt
    20521 ttatgtaagt ctgaatcagt ccctgaatcg ccacgccaaa aagcgtccct aataccgttc
    20581 caacgccacc gctcaaaagc gtaccgccaa ttaccactga ggcgatagca tccagctcca
    20641 cacctacgcc cgccagcgca tatccggcct gggtataaat cgagaagaca atccccgcca
    20701 gcgttgccag tccggtggag agcatataaa tgcgaatagt ggtgctgcga gtggaaatcc
    20761 ccatcagatt cgccgacgtt gcgttgccgc caatggcgta tacctgatta ccaaaacggg
    20821 tacgatgcgc gaggaatatg ccgataacca ccaccgccaa catcagcagt cccatcgcac
    20881 ttaagcgacc gccgccaggg attttccacg caaggcttga gagcgtgtca taaatgggat
    20941 ggtttatcgg aatcgactct tccgaaacga gatagctgac gccgcgcaaa aagaacatcc
    21001 ccgcaagcgt aatgatgaac gccgggatct tcagggcgtc gatcagaagc cccataaatg
    21061 cgccgaaggc acagcccatc accagcacca gcggaaacgc cagcagcggc gagaggccga
    21121 aatcgccaat cacttttgcc agaaacacgc cggtaaaggc gatcaccgaa ccgacggaga
    21181 gatcgatccc accggagagg atcacaaagg tcatgccaac ggcaatgatc ccaagaaagg
    21241 cgttatcggt caggatattg cagatcactc ttgtggaagc aaaaccagga aactgcgtca
    21301 ggcagtagag atagcccagc acaaagacgc cgatggtgat cataagcggc aagttacgtt
    21361 ttatcacggc tacgcactcc tttaatcaga ctgataaagc gttgcgactg aacaatcagc
    21421 acacaaagca ccaccaccgc tttcaccacc tggttcatct ctggcggaaa gcccgaaagc
    21481 aaaattccgg tattcattcc ctgaataatc agcgccccaa ctcccgaaag cagcaggtta
    21541 aagcgaccgc ccatcagcga tccaccgcca atcaccaccg cgagaatggc atccagctcc
    21601 agccataatc cggcgttgtt ggcatcggca ccgcgaatat ccgccgccac gataatgccc
    21661 gcaatcgccg cacatagccc gctcaacacg taggtgagca tgacgatgat tcgtgtgttg
    21721 actccggcat tttttgccgc ccgaatgttg ataccaacgg cttcgataaa catccccagc
    21781 gccgttttgc gggtcaacag ccagaacagg agaagcgtca gcaccgcgat aatgaccggc
    21841 gttggcagga acaacagcga tccgctgcca aaccatgaga gatccggcga gttaaacgtg
    21901 acgatctgcc cggcggtgat cagttgcgcc acgccgcgcc cggcgaccat caggatcagg
    21961 gtggcgacaa acggctgaat tttgaggatc gctaccagta tgccgttcca caatcccgct
    22021 aggatgccgg tgcccagggc gcttaacaaa acaatcggca ggctgaatcc cgcgaccgtc
    22081 atcgcagccg ttgtggctcc ggcgatagcc atcaccgccc ccacagagag atcgatacca
    22141 ccggtggcga tcaccagcgt cataccaatc gccagtaacg caacgggagc tgcacggtta
    22201 agaatgtcga tagggctacc gaacaaacgc ccatcctgaa gcaccacctg ccagaaatgc
    22261 ggggctacca ggctatcgac cagcagcacc agcaacagtg ccgccagctg cggcatcccc
    22321 gtcggccagt gaaaacgcct ttttggcggc gtagtgtcag ggagagattg aggcatcaca
    22381 ctgttctcct tacgccgcaa tggcgttcat gatcgccgga acggaaagct gtgccagcgg
    22441 gatctccgcc acctgtttgc gatcgcgcat gatgatcacc cggtcggcat agcccaccag
    22501 ctcttccagt tcggaggaga tcaccagcag cgccagaccg tcagcacaca gcgtttcaat
    22561 caggcggatg atctcggcgt gggcaccaac atcaatgcca cgggttggct catcaaggat
    22621 cagaaattgc ggtcgggtca gtagccaacg tgaaagcagc actttttgct gattgccacc
    22681 ggagagaaat tcaatcggtt gttcagttga aggtgtgcga atgccaagct ggcggataaa
    22741 gcgttcggca atctcttgct gctctttgcg ggaaatgggt cgtagccagc cgcgctgggc
    22801 ctggagagcg aggatgatat tttcccgcac cgaggcggcg gcgatgatgc catcggtttt
    22861 cctgtcttcc gggcagaatc cgatgcccag tactgaagcc tgatgtggcg atcgcagggt
    22921 ttgcggtttg cctttgatca acgccgtgcc gctgtcagca ggtttgatac cgaagatcac
    22981 ttcggcggtt tcggtacgtc cggatcccag caagccagcc agaccgacga tctcgccggg
    23041 gcgcacttcg agatcaaacg gtgctatcgt tccttttttg ccgtaatttt tgaacgcggc
    23101 aacgggtttg tcgctcaaca atgttcgccc ggcacgctgt agcacgtggg tatccagctc
    23161 gcgccccagc atcattttta ccagttcgat ctgcggtagc tcgcgcgttt cccgacagcc
    23221 tacgaaactg ccgttgcgta agacagtgat ccgatcgctg acctgataga cctgatcgag
    23281 aaagtgagtg acaaagatca ggctgacgcc gcgatcgcgc aactgacgca tcaggccaaa
    23341 taatagctcc acttcctggg tgtcgaggct ggcggtgggt tcatcgagga tcagcacttt
    23401 ggcggagaga tcgatagccc ggcaaatggc gacgatttgc tgcatcgcga ctgaaaagcg
    23461 gttaagcggt tcgcgcacgt cgaggaagaa accgtaagat gccatcagtt cggtggcgcg
    23521 cttttccatc tctttacggc gtagaaggcc gaaacgtttg ggttcgcgac ctatgaatag
    23581 attatcagca accgacatat tgggtagcag gttgacttcc tgatagacgg tgccaatacc
    23641 gagttgttgt gcatgggcgg tatttttcgg tgagatagcc tggccttcca gccagatggt
    23701 gccgcgatcg gcgtggtata caccagttaa tgctttgatt agcgttgatt ttcccgcccc
    23761 gttttcacca agcagcgcca tgatttcgcc acggcgcagg ctgaaatcaa cgttgtctaa
    23821 cgctttgacg ccggggaaaa atttacttaa tccttcggtg cggaggatct cctggtgttg
    23881 gtcggtcgtc atggtttccc ccggtgtaaa gcgtgctccc agtaggcatg ataagacgcg
    23941 gtaagcgtcg catcaggctt cagtgcacca tcgccggatg cggcgcgagc gccttatccg
    24001 gcctacgaat cggcatcagg catagcaacc ctcaataccc catatttttc ttcttctcta
    24061 actcttcttt tgcggtatca ggcaggtaaa gggtggattt ggttaacgtc agcttttcag
    24121 gcatggtgcc gtcttttttg tatttctcca gcgcatcgaa agcgggtccc gccatgttcg
    24181 gcgtcagttc aacactggcg ttcgcttcgc catccatcat cgctttgtag atgtccggta
    24241 caccgtcaat ggaacccgtg aggatatctt tgcccggttt caggcccgct tctttaattg
    24301 cctgaattgc accaatcacc atgtcgtcgt tatgggcgta aaccatgcag atgtttttgc
    24361 cgttgttttc cgctttgata aagctctcca tgacttcttt gcctttactg cgggtgaagt
    24421 cacctgactg cgagcggata attttgatat ttggcgcatt cttaatggct tcggcaaagc
    24481 ctttcttacg gtcaatggcg acgctggccc caacggtgcc ctgcagctcc accacgttgc
    24541 atggtttgcc attcacttct tttaccagcc agtcaccgat caacttacct tcgaggatgt
    24601 tgtcggcagt aacggtggtc atatagagag atttgtcttt cacatcgatg gaacggtcga
    24661 gcaagaagac cgggatttcg gcatctttcg cctcttttaa taccggttcc caacccgtcg
    24721 caaccactgg agcgataaag atcgcatcca ccccttgtgc aacgaaggaa cgtaccgctt
    24781 taatctggtt ttcctgcttt tgctgaccat cggcaatttt caacgtaatt ccgcgttttt
    24841 cggcctcact tttcgccaca ttggtttctg cggcgcgcca gccggattcc gatccgacct
    24901 gcgaaaatcc tccggttaat ggagctgcca acgccataga cgacatggct gccgagactg
    24961 cagagactac aagtaagcgt ttccacatag ggttgtcctc gtcggggtaa ttattattgg
    25021 tgaaaagatg ttcgcgaaaa aactatagac aattcgttat gtaacggatt gcgttacatc
    25081 acacttcaaa aaagtaaata aaacgttaat cacaagtttg taatcgcttt catctcacta
    25141 tgaaaaatgc ggctacggtt atggattttc ctgctctgta taccgtctta aaactggcga
    25201 aaaaggaaaa tgaagacgaa aacaagcaaa gacattcggc gcgagttggc tataatactc
    25261 ggcacttgtt tgccacatat ttttaaagga aacagacatg agcttactca acgtccctgc
    25321 gggtaaagat ctgccggaag acatctacgt tgttattgag atcccggcta acgcagatcc
    25381 gatcaaatac gaaatcgaca aagagagcgg cgcactgttc gttgaccgct tcatgtccac
    25441 cgcgatgttc tatccgtgca actacggtta catcaaccac accctgtctc tggacggtga
    25501 cccggttgac gtactggtcc cgactccgta cccgctgcag cctggttctg tgatccgttg
    25561 ccgtccggtt ggcgttctga aaatgaccga cgaagccggt gaagatgcga aactggttgc
    25621 ggttccgcac agcaagctga gcaaagaata cgatcacatt aaagacgtaa acgatctgcc
    25681 tgaactgctg aaagcgcaaa tcgctcactt cttcgagcac tacaaagacc tcgaaaaagg
    25741 caagtgggtg aaagttgaag gttgggaaaa cgcagaagcc gctaaagctg aaatcgttgc
    25801 ttccttcgag cgcgcaaaga ataaataagt tcttctagcg caataaccct gaacgccggg
    25861 cttcggttag taagggtttt ttatgcccgc gataaataaa ctctctattc caccatcatt
    25921 attctcagcg gttgcaaggc ttgaacggta agaacaagca aacccgacca ccattttgct
    25981 gttcatagcc acttgctgga agttagccga cctcactcat actcaccgtt caaagcgtgc
    26041 ccccgtttta cccctgcaca tttgtgaacg ccagcagagt tttttatgcg attgcagata
    26101 tggaaagcgg ctcggagaga aaagcaaaag atggaaaagt tacaaaaaaa aatcgcgaga
    26161 tacatcgcat aaaacaacgc cacccgaagg tggcgtgctt ttattattac ttatccctgt
    26221 ctaaccagtc gccgctttca attagcttta atccatcgac gggtcgttga taaacgtaca
    26281 tccatgcact cccgtacggc gtctgaatca actggcgcgc gtattcaccg cccctggtgc
    26341 gcaaggcatc aagttcggcc agcgtggcgt tgtcaatacg ataaacttca ccgtgtaccg
    26401 ttccgttccc cggaactgcg cctggatagt ggcccaggct atacaactgg tagttatcga
    26461 tactgaaatc gcccagtaac tgggcattgg tcatccagtg actgttgcct tgtttgtggc
    26521 gtaaactgcc gtagacaaat attcgcattg ctaaaactcg aactgataga gcaaatccag
    26581 tgcctggtct acaccagaca cggcttccag atatagctta ggcatcaggc gataacgtaa
    26641 cgtgagtgtt gctatagagt caaatatacc cacgccgtat ttcacttgca gacctggcaa
    26701 tacatagccg ctgaccacta cctgggagga gtcgcctact ccctgggtgt cgagcgctaa
    26761 attgcttacg ccaaacgtct cgccgatttt acccacaatc tggccacttt gcgcaacccc
    26821 cagaccaatc agcatcgagg tcattgccgc actgtcgctc tgatcgctct ccagcccttg
    26881 tccacgtagc aaataagaca atgcagcttg ttgcgacatc gccgggtcag agaagatctc
    26941 cgctttcggt tcgtccgcca gaccagtgac gcgaactccg gcaattacgt cgtcttctgt
    27001 agcatccggg ttacgaatag cttcaatatt aagatacggt tgatccggcg gaccagagaa
    27061 cagtaactca cccttacgca caatcagatc ctgaccatag gcatggaagc gcccttcagg
    27121 gatgttgatc tgcccgttca ggcccagccc ttgtttgtcc tgtacgacgt taagatcgcc
    27181 cgtcagccgc gctttcaggc caaaggcgtc aatgcgcaca ttgttgccaa cgtggacaat
    27241 caggttactg ttaatcggaa tcgacgccgt tttcggctct tccggttgca ggttatcgtt
    27301 aagcatcacc acatcgctgg agacgcctac tgcgctttcc ggcagatcgt gcaccacgat
    27361 gcgcgcccac gggacatcca cgcgaccatc gagggtaaac aggtttggtg tagcctcgaa
    27421 tacaacatct ggcgatacat ccattcgtac catcggcggc acggtgatcc gcactttact
    27481 gcctttcgcc gttactcgtg cccgccagtt ttcaatttgg ctccagtcgg catcaccgtt
    27541 caggtagatt tcaccctgct gggtccgtac tgtaccggca agcgtcgagc gcataccgtt
    27601 aaagttgacc gcaagctggc tcggctgcat atcaaacggc ataaagttgc cgtcgatatc
    27661 cacacccgta acctgaagct gaccaaacaa ctgcgggctt tgcacatcac cacccagacg
    27721 caagttggca ctcaccatcc ccgctgcttt ttccccacgg gtaaagatgg ggtttatcat
    27781 cgcaaggttg aagttacgga tattgacgtt gccaccaaga ttacggcggc cttgcggatc
    27841 ggtcacctgc acctgtccat caaactggcc gttattggtc aggcggatgg tccagcccaa
    27901 ttcggcacgg ttgttacgca attccgccgt cagattcagt gtctgaaacg ccaccggcag
    27961 cgccgcatcg ttgacggttt gcgttacctg cacgttacgc cccgaaaggg tgatactgcc
    28021 ctgcggcagc ccctctttcg tggtgtccca ggcaacatcc gctttacccg tgaagatacc
    28081 gctggcctga gtggtttctg gcataaacgg tttcagcatg gcgaggtcga agcggttgag
    28141 attcaccacc gcacgccctt cggcccccgc atcgatagtt tgcggcacgc acagttccgc
    28201 attcgggtta agccagcagt gtggcccgat gctgattttt tgctccttat tgcggtaatc
    28261 cagcgcaata tcgcgggtca gcgaccacgg gccgaccggc gtctggaaac gggtattgct
    28321 aagagttccc ttccagcgtt cttctttgcg atcaaaactt cctgccagat taagctgccc
    28381 ggagacaggc tcgccctgaa tccgcaactg tagctcgtgc tgcttttcgc tgcctttggc
    28441 attcagggtg acgaggttga tatttacatc cggctgcgaa atttgctcaa cgcgtacgtc
    28501 gagtttcccg gcgatctgat cggtggattt aatgtcgcct tccacgcgaa cctgcgccac
    28561 ggaaagttcc tgccagcgca ggccgcgcgc ggtgatatct gccagtagtt gtggcgcttc
    28621 caccgtgccg cgtactttca ccagcccttt cgctgtaccg ccaagccccg gcagcgcgtt
    28681 atccagcccc ggcgcgttga tggtggcatc aagattgaga tcttttaccc ccagctcgcc
    28741 tttcacttcg gcactgtttg gcccgagttc cagatgaagc ccagggatca tccactgcat
    28801 ataactgttg cctttcagcg tgccgtcaac gttcactttg ttctgtttga cgttaccggt
    28861 cagcttcagt tctggcacct ccatctgcca ggtgccgccg tacaggctac cgcgggtttt
    28921 aatcaagcca ttgagtttcg acggccactc cgggaactct ttggcggtgt taatgccatt
    28981 aagcgttagc tcaccgcgcc aactaatggc ctgctgccag tcgagcaacg ccttgagttc
    29041 agttttccct tccagcgccg cgacggtgag tttgtcgaga ttgacctgct gttcattacc
    29101 tttggcatca agggtaatgg tggctggcgg gatctcctgt cccttcactg ccgtacgcat
    29161 agagagcgtg taatcggtca ttttgccggt aagtttcagt ttcagatcat ccgcctgata
    29221 ctgcttctca ccagtgaacg gccagtaaag ctgtttgctg ttcacttcca cgttgagtgg
    29281 caatccggct tccgccagtc gcgtctgggc gcgtaaatcc atatccaccg gaccggaaag
    29341 gttaacgcct atctccagct gttcgcgcag cgcgccgccc actttcagct tcaccttttc
    29401 acctttcaac ggctccacgt tcagtgtgct gttgaggatg atatccaccg gccagttgtc
    29461 tgacagctgc gccgtgccgc tggcgttgac gatcccctgg ttggaatcga tatccagggc
    29521 gtccagttta gtattgccgt caatgctgct cactttcagc agcatggtgc gcacggtgat
    29581 gtccgtgtcg cccgtcacgc gcagctgctc gcccttaaac tcttcaatgt tcaggttaag
    29641 cggcaaatgc acgtcggtca tttccggcaa taccgggcga gaaaagagat ctttcagcgt
    29701 ttcgccgagc ggcttttcat ccggctgcgg attttcaatc ttcggttcga cgacctcttc
    29761 ctgcgccact tccgccactt tcggcagagc aatcagcagg cctttcagcg acgtcggttt
    29821 cagggtcagg gttttctcct gccagttcag gccggaggtg aagtccatca ccgacaccgt
    29881 ggtgtcatca atcttgatgt tgacgttatc cagcgccacc cgtgtcaggg tgatgggata
    29941 cggcgtggag agatccagcg gaccgctatc ttcctcttct tcaacctgtt cagaaggagg
    30001 cattttttta ctgtcgatgt tgacctgaat gtctttcagc gccaggtcat taatacaaac
    30061 gctactgttc cacaggcact caagaccgac cgccagatgc agattacccg cttttaccgc
    30121 cacgcctggc tgctcataac gaacgtcaga caaggtgaga tcgcgccagc cgccggtgac
    30181 gttgccaata tccagtcctg gcacccagcg atccgccgct ttaaatacaa gatgcaggcc
    30241 gctggtggtg cccaccagaa acgccaccga tcccagcaac agtaagataa cgataaccac
    30301 gccgaggctg atttttttcc ataaactcat aattctggcc ccagaccgat gtaaaactgt
    30361 aacccgtgtt cgtctttatc cgcgaccggt acggcaaaat cgagtttgat tggcccgacc
    30421 ggcgattccc agcgtacgcc gcccccggta ccggttttaa agtcgctgcg gcgaatatcg
    30481 cttaccgctt cgccactatc gacaaacacc gcgccccacc attttccggt cacgttgtac
    30541 tggtattcca gcgatccggt tatcagcttc gaggcccctt tcaggtcacc gttggcgtat
    30601 ttcggggcga tagatttata tttgtagccg cgaatactac gatcgcctcc ggcgaagaaa
    30661 cgcagatccg gcggtacttt gtcgaaatca ccggtttcaa tccagcccag cgtgccgcgt
    30721 gtcacaaaac gatggcgatc gtacagtgtg cggatccaga cgttctgcgc ctggaaaacg
    30781 gagaaatcaa catctgaacc ccaggccgtg ttggagtagt cgatagagta gcgttgcgag
    30841 tcgccccagg ttggcatcag gccaccacga gaacgcgtgc ggctaatcat caccccagga
    30901 taaaacagca tcgtggtgtt ggtaatttca ccctgggtaa agtggtcgag gctccagcgc
    30961 aggttaatgg cacgctgcca gtcgctggag agatcccagt agcgagaagc caccagcgta
    31021 gtggagtcag attcggtatc gttcaggtca gtgcgcttaa aaccgccctg caccaaataa
    31081 tattgttcca gtggattctt cagcagcggc attttatagc tgaagtcgag gatctgttcc
    31141 ggcgcggaaa tactggtact ggtggtcaga ctgtgaccat aagagttcat ccacggcttt
    31201 ttccacgtcg ctttcacgcg cggtcccacg tccgtagagt aaccgacccc ggtttcgatg
    31261 gtgttttctg ttcgcggcga aaccacgccc gtcaatggta atactttcgt ttcgcgcgct
    31321 ttatcaaatt gcggtgccac taccaccgaa ttaaaccagc cggtagcaga aagtcggcgg
    31381 ttcagttccg ccagatcttt cgattcatac tcatcgccct ctttaaacgg caccagattt
    31441 tgcaggtatt catcgcggat ttgtgatcct tcaaaggtca catgcccaaa gcggtaacgt
    31501 tcgccactgt tataatcaat atcccagaag gctttatgca ggccgagcgc aatgcccagc
    31561 tgcgctttgg taaattcgct atcgaaataa cctttacgca acgcaatact ggttaaggac
    31621 tttttgaaat tttcataatc gccctggttc agtaccgtac caatagccgg gcgagtatcg
    31681 agcaatttca aatagtcttt atcggtccgc gcgccgccgc gcaataccac atcggtgccg
    31741 ccaattaaca ccggtacacc tggcgtgact ttggcgatca atacctgccg ccctttcttt
    31801 ggcggtggac ggagatcaaa ttcaatggtc ggctggtaat aacccagcgc tttcagacct
    31861 tcacggatgg catcatcgac gcgtgcgcga aagcgacggt ctggcgtcac ttcatcactt
    31921 tcaatcgtag aaagctgcgc acgaacgttc ttttccagct gtcccgataa cccctcgacc
    31981 tgtagacgga cgttcgcggc gacggcagat ccgcttaagc agagtaagct tacacagcat
    32041 aactgtcgga tatagcgcac attttctcct gaatatcctt ttttcctacc cctggaacgc
    32101 cgttaaacgg cttaacaaaa atccagtaat atggattaaa aagcagacta aaccccaaat
    32161 atttcttatg ttttacttta gacctattca cggtggttat tgtgtgcaaa tacgcctctt
    32221 gttacaacct taaccccaat gaccgatttt cgggagagcg acaccatgag tttatttgat
    32281 aaaaagcatc tggtttcccc cgccgatgcc ctgcctggac gtaacacccc gatgcccgta
    32341 gccacgctgc atgcggtcaa cggtcactca atgaccaatg tacctgacgg aatggagatt
    32401 gccatttttg cgatgggttg tttctggggt gtggagcgtc tgttctggca gttacccggc
    32461 gtttacagca ccgccgcagg ctataccggc ggctatacgc caaatccgac ttatcgggaa
    32521 gtgtgctccg gtgatacggg tcatgccgaa gcggtacgca ttgtttacga tccttccgtc
    32581 atcagctatg agcagttgct acaggtattt tgggagaatc acgatcccgc ccagggaatg
    32641 cgtcagggca atgaccacgg cacgcagtat cgttcagcga tttatccgct gacccctgaa
    32701 caggatgccg cagctcgcgc cagtctggaa cgttttcagg cggcgatgct tgccgccgat
    32761 gacgatcgtc acatcaccac ggaaatcgct aacgccacac cgttttatta tgccgaagat
    32821 gaccaccagc aatatctgca taaaaaccca tatggttact gtggaattgg cggaattggc
    32881 gtctgtctgc cgccggaagc atagcgttac gggtacaaat gtagtttgtt gataaagtgc
    32941 tctttgtcta tgcctgatgc ggcttaaacg acttaccagg cctacaaaat cgtccaaatt
    33001 caatatattg caaggactgc gtaggcctga tcggcatagc gcatcaggca gtattgcatt
    33061 tatcagcagt ctgaatttta accctctggc gactttacag taccttacgc tatactagcc
    33121 actgaaaatg tcggttcact ttcttcgaat cggctttcaa tgtgtatttc acacaaatta
    33181 atcaactttc ccttccgagg atctggcctg aaaggtcgga taagatatgt taaacagtat
    33241 tttagtcata ctctgcttga tcgctgtaag tgcgttcttc tcgatgtccg agatctcgct
    33301 tgccgcctca cgcaaaatca aacttaaact gctggctgat gaaggcaata taaatgccca
    33361 acgcgttctg aatatgcagg aaaatcccgg catgttcttt accgtggtcc aaatcggtct
    33421 gaacgcagtg gcgattctcg gcggtatcgt cggtgatgcg gcattttctc cagcttttca
    33481 cagcctgttc tcccgctata tgtcggcaga gctctctgag caactgagct ttattctctc
    33541 tttctcgtta gtgactggca tgtttatcct gtttgcggat ttaaccccga aacgcatcgg
    33601 tatgattgcg ccagaagcgg tggctttgcg tatcatcaac ccgatgcgct tctgcctgta
    33661 cgtttgcacc ccactggtgt ggttcttcaa cggcctggcg aacatgatct tccgtatttt
    33721 caaactgcca atggtacgta aagatgacat cacttctgat gacatctacg cggtagtgga
    33781 agccggtgcg ctggcgggcg tgttacgtaa acaggaacac gagctgattg aaaacgtctt
    33841 tgagctggaa tcccgtaccg ttccgtcttc aatgacaccg cgtgaaaacg tgatttggtt
    33901 tgatctccac gaagatgagc aaagcctgaa gaataaggtg gcggaacatc cgcactctaa
    33961 gttcctcgtc tgtaatgaag atattgacca catcatcggt tatgtcgatt ctaaagacct
    34021 gctgaaccgc gtgctggcta accaaagcct ggcactgaac agcggcgtac aaattcgcaa
    34081 cacgctgatt gtgccggata cgttaaccct ttcagaggcg ttggaaagtt ttaaaaccgc
    34141 aggtgaagac ttcgcggtga tcatgaacga gtacgcgctg gtggtgggga tcatcaccct
    34201 caacgacgtg atgaccacgc tgatgggcga tctggtcggt caggggctgg aagagcagat
    34261 tgtcgcccgt gatgagaact catggctgat tgacggcggc actccgattg acgacgtcat
    34321 gcgcgtgctg gatattgacg aattcccgca gtcgggcaac tacgaaacca tcggcggctt
    34381 tatgatgttt atgctgcgta agatcccgaa acgcaccgat tcggtgaaat tcgccggcta
    34441 caaatttgaa gtggtggata tcgataacta ccgcatcgac cagctgctgg tgacccggat
    34501 cgacagcaag gccaccgccc tttcgccaaa actgcctgac gctaaagata aagaagaaag
    34561 cgtcgcgtaa ccccccagaa acatcaacgg ctcctgaatc aggagccgtc ttattactgc
    34621 atagcacttt ggttaagcca tctctgtttg cagacgcata acctgacggt taacttcgga
    34681 catcactgac aaatgcagct tatccttcac ttttgggata agaatcttac ccttatcaaa
    34741 ttcaaaagcg ccaacgtcct tgatgtataa ccgtccacgg aacaggatct ttacgtactt
    34801 cgccacctga agtgggttgt accgttggaa aattttcatt gttatctcct gctgagtatt
    34861 acgcctgtgc ggtgccacaa tcggcccaac tattatgagg cgcaaatttt aatgcttagt
    34921 gactatagac tatccgggca atgtttccac cgcgtataac tttttttacc taaaggttac
    34981 aattattcag aattatcttt ttaccgaagc gcggtcttca gtataagcat tcatttttca
    35041 tatgatttgt gcgcttgacc gcaaactggc atcacacttg cgggaaattc gataaatagc
    35101 acatatgatt aaaactcaga cccaagtggt cggatcacct gcatatcata agaaggaaac
    35161 accatgaccc tacgcaagat tctggcactc acctgcctgc tgttgccgat gatggcttcc
    35221 gcacatcagt tcgaaaccgg tcagcgagtg ccgccgattg gcattaccga tcggggcgag
    35281 ttggtgcttg ataaagatca gtttagctac aaaacctgga acagcgcgca gttagtggga
    35341 aaagtgcgag tactgcaaca tattgctggt cgcacctctg caaaagagaa aaacgcgacg
    35401 ctgattgaag cgattaaatc agcgaagtta ccgcacgatc gttaccagac caccactatt
    35461 gttaacaccg acgacgcgat tccgggttca gggatgtttg tgcgcagcag tctggagagt
    35521 aataaaaagc tttatccctg gtcgcagttt attgtcgata gcaatggcgt cgcgcgcggt
    35581 gcctggcagc tggatgaaga aagttccgct gtagtggtgc tggataagga tggtcgcgtg
    35641 caatgggcca aagacggggc gctcactcag gaagaggtgc agcaagtgat ggacctgctg
    35701 caaaagttgc tcaaataatg gcaacaacat agattctgca aaaataattt tcccctatcg
    35761 ttatctatgg gataggggaa ttaagtctct gagttcttac gccgaactca tactggtaac
    35821 gctagttgct cttttttaaa cgcctcaatg gtcgacttat atttcttgca aatcttttct
    35881 ttggtaatat tactatctgc ggccagattc gcacttacta tcgcatccac ttgttcggct
    35941 aattccactt gttggcaacc tgcattaacc agaaaattat aaataaggga gtaatcttca
    36001 tcctccaaaa caacagagga accattacga aaacgttcat cccctccata gattttaagc
    36061 gcaaagccca aagattctat gactatggca aaattatcat caattatttt ttcgtgaata
    36121 taccatccat cgcattcttt aattccacct cctggtattt ggagttcaaa tttaccccgt
    36181 tttaatttgc ttactatatc ctccaataag gcataaccta aagacacgtc accaatcgcc
    36241 tggtgaatat aacctttaat ccttttcggt atgtcagact gttttttctg cagaatatca
    36301 caggcataca atcttctgtt ttgttctaac ggaaactcgc ggtttttgaa atccatgaat
    36361 gagttatact catttattgt tatgggaatt tcttcgtatt caaatccact acctcgcaat
    36421 gtcattgtat attcggcacc attatcttta aggctgaaac tgataaggaa cttatccatg
    36481 gcgttaacag ttccattttt tgatgaaagt acctgataac tatttttatc aatctgagtt
    36541 gcgcaacgaa agaactccct caaagttaac aggctatcat aattcgaaat ccaatctttt
    36601 ttcttaaatg catgctggat attgttaaaa aaattgtttg aatgtgtttt tgtatacgat
    36661 gactttacaa ataactccac cgcaccaata aaattattgt gtctgatcct gggtaacata
    36721 tgacaacctt attttatttg gttattttat gtcgccatta aata
//
LOCUS       DADMKF010000025        36331 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.025, whole genome shotgun sequence.
ACCESSION   DADMKF010000025 DADMKF010000000
VERSION     DADMKF010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36331
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.025"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            417..1865
                     /locus_tag="JNC24_001127"
     CDS             417..1865
                     /locus_tag="JNC24_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HAY9157938.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKDAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPFIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIIRGLRVDGVGTMIGGTFNSFLHTSFSQNVGLVSVTRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLKVRTVRMWLLMRKLKKNEHGE"
     gene            1867..1992
                     /locus_tag="JNC24_001128"
     CDS             1867..1992
                     /locus_tag="JNC24_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9157939.1"
                     /translation="MNFLMRVIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(1989..2060)
                     /locus_tag="JNC24_001129"
     CDS             complement(1989..2060)
                     /locus_tag="JNC24_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9157940.1"
                     /translation="MINQVSVYRQPPVLSGCRQVKTI"
     gene            2115..2663
                     /gene="idi"
                     /locus_tag="JNC24_001130"
     CDS             2115..2663
                     /gene="idi"
                     /locus_tag="JNC24_001130"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HAY9157941.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTRLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESNEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPNGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLHGIDATPWAFSP
                     WMVMQAANSEARKLLSAFAQHN"
     gene            complement(2706..4223)
                     /gene="lysS"
                     /locus_tag="JNC24_001131"
     CDS             complement(2706..4223)
                     /gene="lysS"
                     /locus_tag="JNC24_001131"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HAY9157942.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(4233..5331)
                     /gene="prfB"
                     /locus_tag="JNC24_001132"
     CDS             complement(join(4233..5255,5257..5331))
                     /gene="prfB"
                     /locus_tag="JNC24_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HAY9157943.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            complement(5422..7155)
                     /gene="recJ"
                     /locus_tag="JNC24_001133"
     CDS             complement(5422..7155)
                     /gene="recJ"
                     /locus_tag="JNC24_001133"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HAY9157944.1"
                     /translation="MKQQIQLRRRAVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGETLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEADKFELFQQRFG
                     ELVTEWLDPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            complement(7161..7871)
                     /gene="dsbC"
                     /locus_tag="JNC24_001134"
     CDS             complement(7161..7871)
                     /gene="dsbC"
                     /locus_tag="JNC24_001134"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HAY9157945.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     ALQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKEMKEFLDEHQKMTSGK"
     gene            complement(7896..8792)
                     /gene="xerD"
                     /locus_tag="JNC24_001135"
     CDS             complement(7896..8792)
                     /gene="xerD"
                     /locus_tag="JNC24_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HAY9157946.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            8904..9425
                     /gene="fldB"
                     /locus_tag="JNC24_001136"
     CDS             8904..9425
                     /gene="fldB"
                     /locus_tag="JNC24_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HAY9157947.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIVALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            complement(9465..9872)
                     /locus_tag="JNC24_001137"
     CDS             complement(9465..9872)
                     /locus_tag="JNC24_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin CptA"
                     /protein_id="HAY9157948.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(9853..10119)
                     /gene="sdhE"
                     /locus_tag="JNC24_001138"
     CDS             complement(9853..10119)
                     /gene="sdhE"
                     /locus_tag="JNC24_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HAY9157949.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRNRERGPVAI"
     gene            10362..11342
                     /gene="ygfZ"
                     /locus_tag="JNC24_001139"
     CDS             10362..11342
                     /gene="ygfZ"
                     /locus_tag="JNC24_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HAY9157950.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMAEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDEHVLLGVAGFQARAALANIFSELPSKEKQVVKEGATTLLWFEYPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            complement(11538..12197)
                     /locus_tag="JNC24_001140"
     CDS             complement(11538..12197)
                     /locus_tag="JNC24_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAY9157951.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(12361..12672)
                     /locus_tag="JNC24_001141"
     CDS             complement(12361..12672)
                     /locus_tag="JNC24_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HAY9157952.1"
                     /translation="MQPNDITFFQRFQDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTVTLDTLTEKHAEQENMTLTELIKVIADIYPGQTQFYVIEFKC
                     L"
     gene            12717..14150
                     /locus_tag="JNC24_001142"
     CDS             12717..14150
                     /locus_tag="JNC24_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAY9157953.1"
                     /translation="MKKLTLQKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDDMFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKVVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHENPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYCCNPDDVMFAQESMRERYVFTDVQLRGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVITSNGEKL"
     gene            complement(14207..14950)
                     /locus_tag="JNC24_001143"
     CDS             complement(14207..14950)
                     /locus_tag="JNC24_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAY9157954.1"
                     /translation="MAIALVTDGSRGIGRATALLLAKEEYTVAVNYQQNLHAAQEVVN
                     LITQAGGKAFVLQADISDENQVIAMFTAIDQHDEPLAALVNNAGILFTQCTVENLTAE
                     RINRVLSTNMTGYFLCCREAVKRMALKNGGSGGAIVNVFSVASRLGSPGEYVDYAASK
                     GAIDTLTTGLSLEVAAQGVRVNCVRPGFIFTEMHASGGEPGRVDRVKSNIPMQRGGQA
                     EEVAQAIVWLLSDKASYVTGSFIDLAGGK"
     gene            complement(15216..18089)
                     /gene="gcvP"
                     /locus_tag="JNC24_001144"
     CDS             complement(15216..18089)
                     /gene="gcvP"
                     /locus_tag="JNC24_001144"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HAY9157955.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASDVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLTRAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLEID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWPEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAAVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            complement(18208..18597)
                     /gene="gcvH"
                     /locus_tag="JNC24_001145"
     CDS             complement(18208..18597)
                     /gene="gcvH"
                     /locus_tag="JNC24_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HAY9157956.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            complement(18621..19715)
                     /gene="gcvT"
                     /locus_tag="JNC24_001146"
     CDS             complement(18621..19715)
                     /gene="gcvT"
                     /locus_tag="JNC24_001146"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HAY9157957.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQAGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPADRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVLEGI
                     GETAIVQIRNREMSVKVTKPVFVRNGKAVA"
     gene            20005..20088
                     /locus_tag="JNC24_001147"
     CDS             20005..20088
                     /locus_tag="JNC24_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9157958.1"
                     /translation="MSKNTKSKNNDIRKYNAKTEVKLVYFK"
     gene            complement(20162..21364)
                     /gene="ubiI"
                     /locus_tag="JNC24_001148"
                     /gene_synonym="visC"
     CDS             complement(20162..21364)
                     /gene="ubiI"
                     /locus_tag="JNC24_001148"
                     /gene_synonym="visC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HAY9157959.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAADA
                     PPQLRVSAINAASEKLLTRLGVWQDILSRRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPAELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLVLVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGTNPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(21387..22565)
                     /gene="ubiH"
                     /locus_tag="JNC24_001149"
                     /gene_synonym="visB"
     CDS             complement(21387..22565)
                     /gene="ubiH"
                     /locus_tag="JNC24_001149"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HAY9157960.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADYATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVTLHCPDRVANVARTQSHVEVTLESGETLTGRVLVA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGKITHAGKRSAYPLALTRAARA
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            complement(22562..23887)
                     /gene="pepP"
                     /locus_tag="JNC24_001150"
     CDS             complement(22562..23887)
                     /gene="pepP"
                     /locus_tag="JNC24_001150"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HAY9157961.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNELEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIMNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENESELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     DVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            complement(23913..24491)
                     /gene="ygfB"
                     /locus_tag="JNC24_001151"
     CDS             complement(23913..24491)
                     /gene="ygfB"
                     /locus_tag="JNC24_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HAY9157962.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            24659..24988
                     /gene="zapA"
                     /locus_tag="JNC24_001152"
     CDS             24659..24988
                     /gene="zapA"
                     /locus_tag="JNC24_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HAY9157963.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            25030..25213
                     /gene="ssrS"
                     /locus_tag="JNC24_001153"
     ncRNA           25030..25213
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="JNC24_001153"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            25234..25836
                     /locus_tag="JNC24_001154"
     CDS             25234..25836
                     /locus_tag="JNC24_001154"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HAY9157964.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQQEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(25937..25996)
                     /locus_tag="JNC24_001155"
     CDS             complement(25937..25996)
                     /locus_tag="JNC24_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HAY9157965.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(26328..26387)
                     /gene="ibsC"
                     /locus_tag="JNC24_001156"
     CDS             complement(26328..26387)
                     /gene="ibsC"
                     /locus_tag="JNC24_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HAY9157966.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            complement(26616..27848)
                     /locus_tag="JNC24_001157"
     CDS             complement(26616..27848)
                     /locus_tag="JNC24_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HAY9157967.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            complement(28104..28763)
                     /gene="rpiA"
                     /locus_tag="JNC24_001158"
     CDS             complement(28104..28763)
                     /gene="rpiA"
                     /locus_tag="JNC24_001158"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HAY9157968.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            29193..30086
                     /locus_tag="JNC24_001159"
     CDS             29193..30086
                     /locus_tag="JNC24_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator ArgP"
                     /protein_id="HAY9157969.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            30289..32433
                     /gene="scpA"
                     /locus_tag="JNC24_001160"
     CDS             30289..32433
                     /gene="scpA"
                     /locus_tag="JNC24_001160"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HAY9157970.1"
                     /translation="MSNEQEWQQLANKELSRREKTVDSLVQQTAEGIAIKQLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKIDDFAPRLSFF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCRTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEVAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAADHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            32426..33421
                     /gene="meaB"
                     /locus_tag="JNC24_001161"
     CDS             32426..33421
                     /gene="meaB"
                     /locus_tag="JNC24_001161"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HAY9157971.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPFCGNALRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVGQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLLVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRVLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            33432..34217
                     /gene="scpB"
                     /locus_tag="JNC24_001162"
     CDS             33432..34217
                     /gene="scpB"
                     /locus_tag="JNC24_001162"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HAY9157972.1"
                     /translation="MSYQYVNVATINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMMPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            34241..35719
                     /gene="scpC"
                     /locus_tag="JNC24_001163"
     CDS             34241..35719
                     /gene="scpC"
                     /locus_tag="JNC24_001163"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
                     /protein_id="HAY9157973.1"
                     /translation="METQWTRMTADEAAEIIQHNDMVAFSGFTPAGSPKALPTAIARR
                     ANEQHEAKKPYQIRLLTGASISAAADDVLSDADAVSWRAPYQTSSGLRKKINQGAVSF
                     VDLHLSEVAQMVNYGFFGDIDVAVIEASALAPDGRVWLTSGIGNAPTWLLRAKKVIIE
                     LNHYHDPRVAELADIVIPGAPPRRNSVSIFHAMDRVGTRYVQIDPKKIVAVVETNLPD
                     AGNMLDKQNPMCQQIADNVVTFLLQEMAHGRIPPEFLPLQSGVGNINNAVMARLGENP
                     EIPPFMMYSEVLQESVVHLLETGKISGASASSLTISADSLRKIYDNMDYFASRIVLRP
                     QEISNNPEIIRRLGVIALNVGLEFDIYGHANSTHVAGVDLMNGIGGSGDFERNAYLSI
                     FMAPSIAKEGKISTVVPMCSHVDHSEHSVKVIITEQGIADLRGLSPLQRARTIIDNCA
                     HPMYRDYLHRYLENAPGGHIHHDLSHVFDLHRNLIATGSMLG"
     gene            complement(35716..36327)
                     /locus_tag="JNC24_001164"
     CDS             complement(35716..36327)
                     /locus_tag="JNC24_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAY9157974.1"
                     /translation="MIGIDNTYPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLFD
                     RQLDFIISPQHVSARVQELENLTISELPPLRLGFLVSRRYEERQEQELLQELPWLQMR
                     FQNRANFEAMIDANMRPCGINPTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNPA
                     TLKYFDAPHRPMYMQEYLYSIRNHRYTATMLQHIAEDRDGTNH"
BASE COUNT         8703 a         9741 c         9170 g         8717 t
ORIGIN      
        1 tgagttatgt cttctcaaat ttcgcgatgc aataaacggg catatattag gtagcatgac
       61 cgttttaatt ataattttgc gactgagttc aaattattgc ccctcaatct atgccgcttt
      121 cattatttca acattgccga tcaaaagagc gctatccaga taaaaacgat taaatgcgag
      181 agtgcgacat gccagaatga ttaatgaaat atcaatatga gaaaattaca gataattcca
      241 ttgtcaggtt ctcttgtcat tttcgtggtt taagcacgat tatgtgattg cctccgcaat
      301 tcctgtctct aactcccctt cctcgcaaaa actggcactc cacgagcatg tgtttagaca
      361 gtttcattaa cgtaaacggt tgctttttac tctggcgggc gaaaggagaa acactgatga
      421 gcgccataga ttcccaactt ccctcatctt ctgggcaaga ccgcccaact gatgaggttg
      481 accgcatatt atcaccagga aagctgatca tactcggtct gcaacacgtc cttgtcatgt
      541 acgcaggtgc agtcgctgtt cctcttatga ttggtgaccg actgggcctc tcaaaagatg
      601 ctattgcgat gctcattagc tcggatctct tttgctgcgg gatcgtcaca ttattgcaat
      661 gtatcggtat cggccgcttt atggggatcc gcctgccggt gattatgtcg gtgacctttg
      721 ctgctgtaac accaatgata gccattggga tgaacccgga tatcggcctg ctggggatat
      781 ttggtgccac tatcgccgcg ggttttatca ccacattatt agcgccattt attggtcgct
      841 tgatgccttt attcccgcca ctggttaccg gtgtggttat tacttctatt gggcttagca
      901 tcattcaggt gggtattgac tgggccgccg gaggtaaagg gaatccgcaa tatggtaatc
      961 ccgtttattt aggtatctcc tttgctgtct taatttttat cttgctcatt actcgctatg
     1021 cgaaaggatt tatgtccaac gtcgccgtat tactggggat tgtatttggc tttttacttt
     1081 cgtggatgat gaatgaagtc aatttatccg ggctacatga tgcttcatgg tttgcgattg
     1141 tcacgccgat gtcatttggt atgccgattt tcgatcccgt ttccattctg accatgactg
     1201 ccgtgttaat catcgtgttt atcgagtcga tggggatgtt cctggcactg ggtgaaatag
     1261 tcggtcgtaa actctcttca cacgatatta ttcgcgggct gcgtgtcgat ggcgtaggga
     1321 caatgatagg cggaacgttt aacagcttcc tccacacgtc attttcacaa aacgttggcc
     1381 tggttagcgt gacgcgcgtt catagccgct gggtgtgtat ttcttcggga attatattaa
     1441 tcctgtttgg catggtgcca aaaatggcgg tgctggtcgc ctccattccg caatttgtgc
     1501 tgggcggtgc tgggctggtg atgttcggca tggtactggc gacagggatt cgaattctgt
     1561 cgcgctgtaa ctacaccacc aaccgttaca acctctatat tgtggcgatc agtctcggcg
     1621 ttggcatgac tccgacgctc tctcacgatt tcttttctaa gttaccggcc gtactgcaac
     1681 cgttgctgca tagcggcatt atgctcgcaa cccttagcgc cgttgtgctg aatgtcttct
     1741 ttaatggcta ccagcatcat gctgacctgg tgaaggaatc cgtctctgat aaagatttaa
     1801 aagtcaggac agtacgtatg tggcttctga tgcgcaagct gaagaaaaat gagcatggag
     1861 aataatatga attttttaat gcgcgttata ttcagtctgc tgttgctttt tactctctct
     1921 attcctgtca tttctgactg tgttgcaatg gccattgaaa gtcgcttcaa atatatgatg
     1981 ctactttttt aaatggtttt tacctgtcgg catccgctca aaacgggcgg ttgtcgataa
     2041 acgctcactt ggttaatcat ttcactcttc aattatctat aatgatgagt gatcagaatt
     2101 acatgtgaga aattatgcaa acggaacacg tcattttatt gaatgcacag ggagttccca
     2161 cgggtacgct ggaaaagtat gccgcacaca cggcagacac ccgcttacat ctcgcgttct
     2221 ccagttggct gtttaatgcc aaaggacaat tattagttac ccgccgcgca ctgagcaaaa
     2281 aagcgtggcc tggcgtgtgg actaactcgg tttgtgggca cccacaactg ggagaaagca
     2341 acgaagacgc ggtgatccgc cgttgccgtt atgagcttgg cgtggaaatt acgcctcctg
     2401 aatctatcta tcctgacttt cgctatcgcg ccaccgatcc gaatggcatt gtggaaaatg
     2461 aagtgtgtcc ggtatttgcc gcacgcacca ccagtgcgtt acagatcaac gatgatgaag
     2521 tgatggatta tcaatggtgt gatttagcag atgtattaca cggtattgat gccacaccgt
     2581 gggcgttcag tccgtggatg gtgatgcagg cagccaatag tgaagcaaga aaattgttgt
     2641 ctgctttcgc gcagcacaat taaaaaaccc cgacatttgc cggggttgtg agcataacgt
     2701 aatgcttatt ttaccggacg catcgccggg aacagaataa cgtcgcggat ggtatggctg
     2761 ttggtgaaca gcattaccat acggtcgatg ccaatcccca gacctgctgt cggcggtaag
     2821 ccatgttcca gtgcggtaac gtaatcttca tcgtagaaca tcgcttcgtc gtcacctgcg
     2881 tctttcgcgg caacctgatc caggaagcgt tgcgcctgat cttccgcgtc attcagctcg
     2941 ctaaagccgt taccgatttc acgaccaccg atgaagaact caaagcggtc tgtgatttcc
     3001 gggttaatgt cgttacgacg cgccagcgga gaaacttctg ccggatattc agtaatgaag
     3061 gtcggctgaa tcagatgtgc ttctgccact tcttcgaaga tctcggtaac gatacggccc
     3121 agaccccagc tcttctcaac gtggataccg atagattcag caatcgcttt cgcagagtcg
     3181 aagttatcca gatccgccat gtcggtttcc gggcgatatt tcttgatcgc ttcacgcatg
     3241 gtcagttttt cgaacggttt accgaagtcc agcgtcacgt cgccgtaggt cacttccgtc
     3301 ttaccgagaa tatcctgtgc cagagtacgg aacagcgatt cggtcagctc gatcagatct
     3361 ttgtaatctg cgtaagccat gtagagttcc atcatggtga actctgggtt atgacgtacg
     3421 gaaatacctt cgttacggaa gttacggttg atttcgaata cacgctcgaa gccaccgacc
     3481 accagacgct tgaggtacag ttccggcgcg atacgcaggt acatgtcgag atccagcgcg
     3541 ttatggtggg tgataaacgg acgcgcagcg gcaccgcctg ggatcacctg catcatcggc
     3601 gtttcaactt ccataaagcc gcggttcacc atgaactggc gaataccaga gaggatctgc
     3661 gagcgtactt taaaggtgtt acgggattca tcgttagaga tgagatccag ataacgctga
     3721 cgatagcgcg cttcctgatc ctgcaagccg tggaatttat ccggcagcgg acgcagtgct
     3781 ttggtcagca gacgcagctc ggtacagtgg atagacagtt cgccggtttt ggttttgaac
     3841 agtttacctt tcgcgccgag gatgtcgccg aggtcccatt ttttgaactg ctcgttgtaa
     3901 acgccttccg gcagatcgtc acgggcaacg tacagctgaa tgcggccacc cacatcctgc
     3961 agggtaacga aagacgcttt acccataata cggcgggtca tcatgcggcc agcaacggcg
     4021 acttcgatgt tcagcgcttc cagttcttcg ttctctttac cgtcgaattc tgcgtgcaat
     4081 tggtcagagg tatgatcgcg acggaaatcg ttcgggaagg caatcccctg ctcgcgcagg
     4141 ttcgccagct tctcacgacg cgttttcagt tcattgttaa gatcgactac cgcgtcagcg
     4201 ccctgtgcgt gttgttcaga catgttggtt cctcataacc ctgctttcaa acttgcttcg
     4261 ataaattgat ccaggctgcc gtccagcacg gcctgcgtgt tgcgagtttc taccccggta
     4321 cgcagatctt taatgcggga gtcatcaagg acataagaac gaatctggct gccccagccg
     4381 atgtcagatt tgttatcttc catcgcctgt ttctcggcat ttttcttctg catctccagt
     4441 tcataaagct tcgctttcat ctgcttcatg gcctgatctt tgttcttgtg ctgggaacgg
     4501 tcgttctggc actgggtcac gatcccggtc gggatgtggg taatacgcac cgcagattcg
     4561 gtacggttaa cgtgctgacc gcccgcgccg gacgcgcgat aaacgtcaat gcgcagatcc
     4621 gccgggttga tttcgatatc aatatcatca tcaacttccg gataaacaaa cgcagagctg
     4681 aacgacgtgt ggcgacgacc gccggagtca aacgggctct tacgcaccag gcggtgaacg
     4741 ccagtttctg tacgcagcca gccgtaagcg taatcgccgg agattttgat cgtcacggat
     4801 ttaatacccg ccacttcacc ttccgactct tcgatgattt cagttttgaa accacgcgat
     4861 tctgcccagc gcagatacat acgctcaagc atgctcgccc agtcctgtgc ttccgtaccg
     4921 ccagaccccg cctgaatatc gaggtagcag tcggcgctgt catattcgcc agagaacata
     4981 cggcggaact caagctgcgc cagtttttct tccagggcgt cgagttcagc aacggcttcg
     5041 ttaaaggttt cttcgtcgtc agcttctaca gccagttcca gcagaccaga aacatcttcc
     5101 agcccctgtt tcatttggtc gagggtgtcg acaacggctt cgagggagga acgctcttta
     5161 cccagcgcct gtgcgcgttc gggttcattc cagacatccg gctgttccag ctcggcgttt
     5221 acttcttcca gacgctcttt cttggcgtcg tagtcaaaga taccccctaa gaacgtcgga
     5281 gcgttccgtg aggtcctgaa tgcgattatt taccggatta atttcaaaca tggtctgatt
     5341 tcttttattg agctagtcaa aatgcggtga taagagcggg attgtaccca atccacgctc
     5401 ttttttatag agaagatgac gctaaattgg ccagatattg tcgatgataa tttgcaggct
     5461 gcggttgccg cgaaactcgt tgatatcgag cttgtaagcc agttgcactt cgcgcacgcc
     5521 gttatccggc cagagggcgg tatcgacatt aaaagcaata ccatccagca gcggaccgcc
     5581 gccgaccggt tcgaccatga ctttcaaatg acgttcgccc accagccgct gttgcagcag
     5641 ccggaaatga ccgtcaaaca gcggctccgg gaacatctgc ccccacgggc cagcatcgcg
     5701 cagcagctgc gccacttcca tggtcatttc ggccgggctt aacggaccgt ctgacaccac
     5761 ttcgccttgc aatagcgaag ggtccagcca ctcagtaacc agttcgccaa accgttgttg
     5821 aaagagttcg aatttatccg cttccagcga caaacccgcc gccatcgcat gaccgccaaa
     5881 cttcagcatc atgccagggt agagtgtgtc taatcgctcc agcgcatcac gcatatgcag
     5941 cccctgaatg gagcgaccag aacctttcag cgtaccatca cccgcgggcg caaaggcgat
     6001 aaccggacgg tgaaaacgct ctttaatacg ggaagccaga ataccgacaa ccccctgatg
     6061 ccattcgggg tgatacattg ccagcccgcc gggtagcgtg tcacggctgc gctccagttt
     6121 ctcgcacagg gtcagggctt caacctgcat tccttgttcg atctcttttc gcgtctggtt
     6181 tagcgcatcg agttcatttg ccagcacgcg cgcttcgccg atgttgtcgc acagcaagag
     6241 cgccacaccg acggacatat cgtccagtcg tccggcagca ttgagacgtg gccccagcgc
     6301 aaaacctaaa tcgctggcgg cgagtttttg tgcatcacgg tttgccactt caagcagcgc
     6361 tttaatcccc ggacggcact ttccggcacg gatgcgactc atcccctgcc aggtcagaat
     6421 gcgattatta gcgtccagcg gtacgacgtc cgccactgtc cccagcgcta ccagatccag
     6481 cagttctgcc aggttaggaa ttgcgatgcc gcgctcatca aaccagccct gatcgcgcaa
     6541 aaaggtgcgc agcgccagca tcagataaaa cgccacaccc acgcctgcca gtgatttcga
     6601 cgggaaatta cagtcgcgca agttagggtt aatgatcgct tccgccgcgg gtaatgtttc
     6661 gcccggcaaa tggtgatcgg taacaataac cggaatgccc aacgagcgag cgtgttcaac
     6721 ccccgcatgg gaggaaatac cgttatccac cgtgacaatt aactgcgcac cacgggcatg
     6781 ggcctgatcg accacttccg ggcttaagcc gtaaccgtct tcgaaacggt ttggaaccag
     6841 gtagtcgata ttgctgcaac caagcgagcg catcgccagc acgcttagag ccgtgctggt
     6901 ggcaccgtcg gcgtcgaaat caccgaccac aataatccgc gttccttcgc gaaaggcgtt
     6961 gtaaaggatc tcaacggcct tttcgacgcc gctcagttgc tgccagggca gcatcccttt
     7021 aacactgcgt tccagttctt gcgcactgcg cacgccccgg ctggcgtata aacggcgcag
     7081 caagggaggc aattcagcgg gcaagtctgc cgtttcatcg actgcacggc gacgaagttg
     7141 tatctgttgt ttcacgcgaa ttatttaccg ctggtcattt tttggtgttc gtcgaggaat
     7201 tctttcatct ctttcggcgg ctggtaaccc ggaacaagtg tgccattgct cagcacaact
     7261 gccggagtac cgctaacgcc aagctggacg ccaagtgcgt aatggtcggc aatatccacg
     7321 tcgcagctgg ctggtgcgac gcttttacct gccatcacat catcaaacgc tttgttttta
     7381 tctttcgcac accagatagc tttcatttct ttctctgcat cgctgtccag cccctggcgc
     7441 gggaaagcaa gataacgcac ggtgatcccc agcgcgttat agtctgccat ttgctcatgc
     7501 agtttgtggc agtaaccaca ggtaatatca gtaaacacgg tgatgacgtg tttttcctgc
     7561 agcgctttat aaacgatcat ctctttttca agcgcattca actgctttaa cagcatctta
     7621 ttggtgacat tgaccggagc cgtgccgcta acgtcataca ttggcccctg aatgatatgt
     7681 ttaccatcat cggtgatgta caacacgccg ctgttagtca gaactgtctt catgccagct
     7741 acgggcgcgg gctgaatatc gctgcttttg atgcccattt tggctaacgt ttgttgaatt
     7801 gccgcgtcat cagcctgagc aaaacctgaa aacgccgcta acaaagtaaa caacataaaa
     7861 cctttcttca taaatcttcc cgttcttttc agacatcacg cccgcgggtg atgctgttga
     7921 tgaagttgcc gcagacgctc ggtagcgaca tgcgtataaa tttgcgtggt ggaaagatcg
     7981 ctgtggccca gtagcatctg caccacgcgt aaatccgcac catgatttaa taaatgagtg
     8041 gcaaaagcgt gacgcaacac atgcggtgac agcttttcgc tgtcgatacc cgccagcacg
     8101 gcataatgtt taatgcggtg ccagaaggtc tgtcgcgtca tctgctgcgc acgctggctg
     8161 ggaaacaaca cgtcaattga cacaccattc agcagccacg gacgcccatg ttccagatag
     8221 gtttccagcc agtaaaccgc ctcttcacct aacggtacca ggcgctcttt gttgccttta
     8281 ccaatgaccc gcactacgcc ctgacgcagg ctgatatcac tcattgtcag cccgaccagt
     8341 tcagagacac gcagcccggt agcatacaac acttcaagca tggctttatc gcgtagctcc
     8401 agtggctgat caattaatgg cgcctgtaat aaacgttcga cctgcgcttc gcttaaatct
     8461 tttggcaaac gctggggcaa tttcggtgaa gcgagatgcg cactgggatc gtcttcacga
     8521 aacttttcgc gataaagata ctggaacaat cggcgcactg cactcagcaa acgcgctgag
     8581 ctggtggctt tatacccgcc ctccagccgt tctgccagta atgcctgcaa atcgtcactt
     8641 tgcgccgtcg ccagcgtcaa cccgcggtga tgcaaccact ccaccatcat tgacagatcg
     8701 cgacggtaag cgttcaacgt attttcagcc agattttttt ccagccacag agcatcaaga
     8761 aactgctcga tgcgtgccag atcctgtttc acttgcgccc cttatggtca ctcatttgat
     8821 ccattatgcc ttattgtgcc gtgactaaag cgattctgat acactagctg caaaagccac
     8881 agcagaatcg agaagcttac gttatgaata tgggtctttt ttacggttcc agcacctgtt
     8941 acaccgaaat ggcggcagaa aaaatccgcg atattatcgg cccagaactg gtgaccttac
     9001 ataacctcaa ggacgactcc ccgaaattaa tggagcagta cgatgtgctc attctgggta
     9061 tcccgacctg ggattttggt gaaatccagg aagactggga agccgtctgg gatcagctcg
     9121 acgacctgaa ccttgaaggt aaaattgttg cgctgtatgg gcttggcgat caactgggat
     9181 acggcgagtg gttcctcgat gcgctcggta tgctgcatga caaactctcg accaaaggcg
     9241 tgaagttcgt cggctactgg ccaacggaag gatatgaatt taccagcccg aaaccggtga
     9301 ttgctgacgg gcaactgttc gtgggtctgg cgctggatga aactaaccag tatgacctta
     9361 gcgacgagcg tattcagagc tggtgcgagc aaatcctcaa cgaaatggca gagcattacg
     9421 cctgatctca ctgacggctt agcgcatatg ctttgccgtc atctttatct ttgcgtctct
     9481 tgttgcaaca aaatccgccg taaatcccgc cattcggctt cgtccatgct gtcggctgcc
     9541 agccataaat gttgccgttt accgccatca gaacgtaaac gcagcatcat gccgctctta
     9601 atcatccacg gtgctttgac gatgctccac tcctgccctt gccaacgcaa acgcccgtcc
     9661 atcaacaagc gaatttcccc ctggcgagca ttaatacgcc gctggctgcg aacgcaatca
     9721 aacaccacca gcgaaagtaa caccatccat aacggggtgt aactgagtgg ccagggcatg
     9781 agtaaaataa cagcggcaac cagcccatga atcagcaagg aaagccactg tgcgcgccag
     9841 gagacgcgca aatcagattg ccacaggacc acgttcccgg ttccgtgtct ggatgagtcg
     9901 gaccatcatt tccagttctg catcggccgg tttaccgtga ttcatcagcc agttaaacag
     9961 gtccggatcg tcacattcca gcagacgaat aaagatgcgt ttttcgtcat cgcttaagct
    10021 gtcgtactca tgttcgaaaa acggcatgat tgaaatatcg agttcgcgca taccacggcg
    10081 gcatgcccaa tgaatgcggg ctttgttgtt aatgtccatc ttcttcctgt ctcacgaaaa
    10141 tccagtaccc cgctattgta acgtgttttt cgccttcttt tacgggaata tcagtaaaca
    10201 ccatcgcgat cacgaaatta attcacaaca attcaatggc ttcatttttt tggaagtcgc
    10261 ctcgcagaag gcacagatcg cgtagtgaaa gcacttgcat tgcctcatag ctcttttacc
    10321 attagtcatt aatacgccgt taagcaactc aggactctat tatggctttt acaccttttc
    10381 ctccccgtca gcctacggct tctgcccgtt taccgctgac gctgatgacg cttgatgact
    10441 gggcgcttgc caccattact ggcgcggaca gcgaaaaata tatgcagggt caggtgacag
    10501 cagatgtcag ccagatggca gaagatcagc acctgctcgc cgcccattgc gacgccaaag
    10561 gcaaaatgtg gagcaattta cgtctgttcc gcgacggcga tggctttgcg tggattgaac
    10621 ggcgcagcgt gcgtgaaccg cagctgactg aactgaaaaa atatgcggtg ttctctaaag
    10681 tgaccatcgc gccagacgac gagcatgtgc tgcttggtgt tgccggtttt caggcgcgcg
    10741 ccgcgctggc aaatatcttt agtgaactgc cttcgaaaga aaaacaggta gttaaagaag
    10801 gcgcgaccac tttgctatgg tttgaatacc cggcagaacg tttcctgatc gtaaccgatg
    10861 aagctactgc taatatgctg accgataaac tgcgcggtga agcggaactg aacaatagcc
    10921 aacagtggct ggcattaaac attgaagcgg gtttcccggt gattgatgcc gccaacagcg
    10981 ggcagtttat cccacaggcg accaatctcc aggcgctggg cggtatcagc tttaagaaag
    11041 gctgttatac cggacaagag atggtggcgc gagcaaaatt ccgtggtgcc aataaacgtg
    11101 cgctctggtt gctggcaggt agcgccagcc gactgccgga agctggtgaa gacttagagc
    11161 tgaaaatggg cgagaactgg cgtcgtaccg gtacggtgct ggctgcggta aaactggaag
    11221 atggtcaggt cgtggtacag gtcgtcatga ataacgatat ggaaccggat agcatcttcc
    11281 gcgtacgcga cgatgcgaat acattgcata ttgagccgct gccgtattcg ctcgaagagt
    11341 aaatctcttt atcgcatcag gcatttatcg cctgatgcga cgctgacgcg tcttatcatg
    11401 tctgggattt gatgccttta tcacaacagg gacgaggccc gtagatcgga taaggcgttc
    11461 acgccgaatc cggcagttgt gctccgatgc ctgatgcgac gctgacgcgt cttttcaggc
    11521 ctacaaaggc atacccatta cgcctgccca atatacaaat agatcgccag aaagtggcac
    11581 acactgccgc cgagcacgaa gccgtgccag atggcatgat tgtagggaat gcgtttgcag
    11641 acatagaaaa tcactccgag cgaataaacc acgccgccta ccgccaataa ggtaacgctg
    11701 cccgccgcga gcttaactgc catttcataa attaccacca gcgacagcca gcccatcgcc
    11761 agataggtca ccagagataa aattttgaat cggtgcgcga tggtcagttt aaacagaata
    11821 cccagcaatg ccaggctcca gataacaatc atcaacccgc gcgctaacgg agaatccagc
    11881 cccaccagca aaaacggcgt gtaggttccg gcaatcaaca ggtaaatagc gcaatggtca
    11941 aatttcttca gccacatttt tgcccgttga tgaggaatgg cgtgatagag cgtcgaagcg
    12001 aggaacagca ggatcatact gccgccatag aggctgtaac tggttatcgc tgtggcgctg
    12061 gcattgagat ccaccgcctg aaccagtagc aacaccagcc caacgatacc aaacaccaac
    12121 ccaatgccgt gactgacgct gttggcaatt tcctctgcca gcgaatatcc ctgcttaatg
    12181 aggggcttct gaaccataac ttactccgga gaaacgtaca cgcacatgta tacctctcca
    12241 gcgtaactga gaatagttcc agtgaacacc tgttagctaa aataaattct gatttgagac
    12301 taatctccta aaaatcatga aattaaatgc gaaatttcaa ctaacaggcg tgagttcaat
    12361 ttaaagacat ttaaattcaa tcacataaaa ctgtgtctga ccgggataga tgtcggcaat
    12421 gacctttatc agttcagtca gggtcatatt ttcctgctct gcatgttttt ccgtcagcgt
    12481 atccagcgtt acggttgagg ttgcggtgac ttcaatcgtg caaaaataac cgtcatcttc
    12541 aaaacgcccg acacgaagca catcacccgt tttgaagtgc gattcagact cgtcgcggat
    12601 ggtgatggtt ttacgcccag ccagaatgtc atcctggaaa cgttgaaaaa aagtgatgtc
    12661 gtttggctgc atggtactat ttcctgtaag aattgactca tctggagcct atgatagtga
    12721 aaaaactcac cttacagaaa gatttcttat ggggcggcgc agttgccgct catcaggtcg
    12781 aaggcggctg gaacaaaggc ggcaaagggc cgagcatttg tgacgttctg accggtggcg
    12841 cacacggcgt gccgcgcgaa atcaccaaag aagtcttgcc aggcaaatac tatccaaacc
    12901 atgaagccgt tgatttttat ggtcactaca aggaagacat caagctattt gccgaaatgg
    12961 gcttcaaatg ttttcggact tccatagcct ggacgcgcat ttttccaaaa ggcgatgaag
    13021 ctcagccaaa cgaagaaggg ctgaagttct acgatgatat gttcgatgaa ctgctgaaat
    13081 acaacatcga accggtgatc accctctccc actttgaaat gccgctgcat ctggtgcagc
    13141 aatacggtag ctggaccaac cgtaaagtgg ttgatttctt tgtccgtttc gcggaagtgg
    13201 tgtttgaacg ctataagcat aaagtcaaat actggatgac cttcaacgaa attaacaacc
    13261 agcgtaactg gcgtgcaccg ctgttcggtt actgctgctc cggcgtggtt tataccgagc
    13321 atgagaaccc ggaagagacg atgtatcagg tgctgcatca ccagtttgtc gccagcgccc
    13381 tggcggtgaa agctgcgcgt cgcattaacc cggagatgaa agtcggctgt atgctggcga
    13441 tggtgccgct ctatccctac tgctgtaacc cggacgatgt gatgttcgct caggagtcga
    13501 tgcgcgaacg ctacgtcttt accgatgtgc agctacgcgg ctattacccg tcctatgtgt
    13561 tgaacgagtg ggagcgtcgc ggatttaaca tcaaaatgga agacggcgat ctcgatgtac
    13621 tgcgcgaagg cacctgcgat tatcttggtt tcagctatta catgaccaac gcagtgaagg
    13681 ccgaaggcgg caccggcgat gcgatctctg gtttcgaagg cagcgtaccg aacccgtatg
    13741 ttaaagcgtc tgactggggc tggcagattg atccagtagg tctgcgctat gccctttgcg
    13801 aactgtatga gcgttatcag aggccgctgt ttattgtcga aaacggtttt ggcgcttacg
    13861 acaaagtgga agaagatggc agcatcaacg acgactaccg cattgactac ctgcgcgccc
    13921 atatcgaaga gatgaaaaaa gcggtgactt acgatggcgt ggatctgatg ggctacacac
    13981 cgtggggctg catcgactgc gtatcgttca ccaccgggca gtacagcaaa cgctacggct
    14041 ttatctatgt gaataaacat gacgacggta ctggcgatat gtcgcgttca cgtaagaaga
    14101 gctttaactg gtacaaagag gtgattacca gcaacggcga gaagctttaa gtcgatgaag
    14161 taccggatgc aatacttgtt gcatccggtc agacaacttc cctgttttat ttcccgcccg
    14221 ccaaatcgat aaaacttccc gtgacgtaag aggctttatc acttagtagc cagacaatgg
    14281 cctgcgcgac ctcttctgcc tgtccaccac gctgcatggg gatgttcgac ttaacgcgat
    14341 cgacgcgtcc aggctcgccg ccgctggcgt gcatttcggt aaaaataaac cctggccgca
    14401 cgcagttaac gcggaccccc tgcgcggcga cttccagcga tagtccggtg gttaacgtat
    14461 caatcgcccc tttcgatgcc gcgtaatcaa catattcccc tggcgaaccc aaccgtgagg
    14521 ccaccgaaaa gacattgacg atagcgccgc cactgccacc atttttaagc gccatgcgtt
    14581 ttaccgcctc gcggcagcag agaaaatatc ccgtcatgtt ggtggaaagt actcggttga
    14641 ttcgctctgc ggtaaggttt tcaacggtgc actgggtaaa caagatcccg gcgttattga
    14701 ccagcgctgc tagcggttca tcgtgctgat cgattgctgt aaacattgcg atgacctgat
    14761 tttcgtcgct gatatccgcc tggagcacga atgccttgcc accggcttgc gtgattaagt
    14821 tcaccacttc ctgcgccgca tggaggtttt gttgataatt aaccgccacc gtatactctt
    14881 ctttcgccaa cagtaatgca gttgcccgcc cgatgccgcg actgccatcg gtcacaagtg
    14941 ctatagccat ttgtttttcc gagaaaaatt aattcgttga cgtatctttg agattgctaa
    15001 caaactggct caatcttcaa tgttggcacg cttttcccct cgccctttca gggagagggc
    15061 cggggtgagg gtaaatattc gcgccagtgc cggcctgttc ccctcaccct aaccctctcc
    15121 ccaaaggggc gaggggactg tctgagcact tttgtacttt gtcatctgac taaaaaggcg
    15181 ccgaagcgcc tttagaaaat agtcgaatca gtgaattact ggtattcgct aatcggtacg
    15241 caggagcaga acaggttacg gtcgccgtaa acatcatcca gacgtttcac cgtcggccag
    15301 tatttgtctg ccacgcctgc cgggaatacc gcaacttcac ggctgtacgg atgcgcccac
    15361 tcggcgacca gttcgctctg aatgtgcggc gcgttcacca gcgggttatc ttccagcggc
    15421 cagacagcgg ctttcacctg gtcaatttct gcgcggatag ccagcatcgc gtcgataaag
    15481 cgatccagtt ccactttgct ttcagattca gtcggttcaa ccatcagcgt acccgccacc
    15541 gggaacgaca tcgtcggcgc gtggaaaccg tagtcgatca ggcgcttggc aatatccagc
    15601 tcgctgatgc cggtttcttc tttcagcggg cgaatatcga gaatacattc gtgcgccacg
    15661 cgaccgtcgc gaccggtata cagcaccggg aaggcatcct gcaggcggct ggcaatatag
    15721 ttggcgttga ggattgccac ctggcttgct tttttcagcc cttctgcgcc catcatgcgg
    15781 atgtacatcc agctgattgg caggatagag gcgctaccga acggtgccgc agaaactgcg
    15841 ccctgacggg ttaacatgcc ttcgatttgc accacgctat gacccggtac aaacggtgcc
    15901 agatgcgctt tcacaccgat cggtcccata cccggaccac caccgccgtg cggaatgcag
    15961 aaagttttat gcaggttgag gtgcgaaacg tccgcgccaa taaagcccgg cgaggtgatg
    16021 ccaacctggg cgttcatgtt cgcgccatca aggtaaacct gaccgccgaa ctgatgcacg
    16081 acttcacaca cttcacggat cgtttcttca tacacgccgt gggtagaagg ataagtcacc
    16141 atgatacagg agaggttatc gcccgcctgt tccgctttcg cgcgcagatc agtcagatcg
    16201 atgttgccgt ttttatcaca cgccacaacc accacctgca ttcctgccat atgtgcagaa
    16261 gcggggttag ttccgtgcgc agaagccggg atcaggcaga tatcgcgatg cccttcgttg
    16321 cggctttcat gataatgacg aatcgccagc aggcccgcgt attcgccctg tgcgccggag
    16381 ttcggctgca tacaaacggc gtcgtaaccg gtcagtttca ccagccagtc agccagctgc
    16441 gcaatcatct gctgataacc ttcagcctgt tccggcgggc aaaacgggtg cagttcggca
    16501 aattccggcc aggtgattgg gatcatctcg gcggcggcgt tcagtttcat ggtgcaggaa
    16561 cccagcggga tcatcgcctg attcagcgcc agatctttac gctccagcga gtgcatatag
    16621 cgcatcattt cggtttcgct gtggtagcga ttaaacaccg gatgggtgag gatttcgtcg
    16681 tcgcgcagca tcgcaggctg gatagagcgg ctgtcgtgag ccacgtcttt gtccagcgtg
    16741 tcgatctcca gcccgtggtt atcgcccagc agcacgctga aaagctgcat tacgttttca
    16801 cgcgtggtgg tttcatcaag ggtgatcccc acggcgttca ggatatcgct acgcaggttg
    16861 atttcagccg cttcggcacg cgtcagtacg cccgctttgt cggccacttc cacacacaag
    16921 gtgtcgaaat agtgcgcatg gcgcagtttc agaccttttt gttgcaggcc cgccgccaga
    16981 atatcggtca gacggtgaat gcggttagcg atacgtttca ggccaaccgg gccgtgataa
    17041 acggcataca ggctggcgat gtttgccagc agtacctggg aagtacaaat gttggagttc
    17101 gctttctcac gacgaatatg ttgctcgcga gtctgcatcg ccatgcgcag cgcggtattg
    17161 ccagctgcat ctttcgatac accgataata cggcccggca ttgagcgttt gtattcatct
    17221 ttcgccgcaa agaatgccgc gtgtgggcca ccgtagccca tcggcacgcc gaagcgttgc
    17281 gccgaaccaa aaacaatatc cgcgccctgt ttacccggcg cagttaacag caccagcgcc
    17341 ataatatcgg cggcaacgct gaccacaatt ttgcgtgatt tcagttcgct aataagcgca
    17401 gtgtagtcgt gaatttcacc ggtagtgcct acctgctgta acagcacgcc gaagacgtcc
    17461 tgatggtcga gcactttttg cgcgtcatcg acaatcactt caaaaccaaa ggtttcggca
    17521 cgagtacgga ccacatccag cgtttgcgga tgcacatcgg aagccacgaa gaagcggttg
    17581 gcatttttca gtttgctgac gcgtttcgcc atcgccattg cttcggcggc agcggtggcc
    17641 tcgtccagaa gagaagcaga ggccatatcc agtccagtca aatccagcgt tacctgctgg
    17701 aagttgagca gtgcttcaag gcggccctgg gagacttcag gttgatacgg agtgtacgcg
    17761 gtataccagc ccggattttc cagcatgtta cgcaggataa ccggcggtag ctgcacggcg
    17821 gtgtaaccca tgccgatgta agacgtgaag cgtttattgc gactggcaat agccttgagt
    17881 tctgccagtg cggcgtattc ggtcgccggt gcgccaacct gcggcggggt cgcaagctga
    17941 atatctttcg gtacaatctg gccggtcagc gcgtttaacg attgtgcgcc aacggcattc
    18001 agcatttctt gctgttgcgc ggcgtccggt ccgatatggc gttcaataaa agcgccgctg
    18061 ttttcaagct ggcttaacgt ctgtgtcatg agcgatggtt cctgaaacgt gcagtgaatt
    18121 gtgaacctct ctccttacga agagagtgag ggtgaggcat aaattttcct caccctgatc
    18181 ctctcccgca gaagaggaat aaagccgtta ctcgtcttct aacaatgctt cgtatgcggt
    18241 cgcatccagc agtgattcca gttcgctttc atcgctggct ttgattttaa agatccagcc
    18301 acctgcatac ggttcgctgt tcaccagttc cggggaatcg ctcagtgcgt cgtttaccgc
    18361 cacgatttca ccgcttactg gcgcataaat gtctgacgcc gcttttactg attcggcaac
    18421 cgcgcagtca tcgcccgcgc taaccgttgc gcccacttcc ggcaggtcaa caaacaccat
    18481 atcgcctaac agctcctgag catgttcggt aataccaacg gtgtaagtgc cgtcggcttc
    18541 tttacgcagc cattcgtgtt ctttgctgta tttcagttct gctggtacgt tgctcatcaa
    18601 tcaatctcca aaaaagtaaa tcacgcgacg gctttgccgt tacgcacaaa aacaggtttt
    18661 gtcactttaa ccgacatttc acggttgcga atttgcacaa tcgccgtttc gccaatacct
    18721 tccagcacgc gcgccagcgc aatgctgtaa cccagcgtcg gggagaaagt accgctggtg
    18781 ataatgcctt catgctggtt gccctgcgca tcggtaaagc gtaccggcag ttcattacgc
    18841 agcacgcctt tttcggtcat caccagacca accagttttt ctgtaccatg ctcacgctgc
    18901 acttccaggg cttcacgacc gataaagtca cgatctgccg gttcccaggc gatagtccag
    18961 cccatgttgg cggctaaagg agaaatagtt tcgtccatct cctgaccata aagattcatg
    19021 cccgcttcca gacgcagcgt gtcacgcgcg cccaagccac atggcttaac acccgcttcc
    19081 accagcgcac gccagaaatc ggccgctttt tcattgggca gcgcaatttc atagcccgct
    19141 tcaccggtat aaccagtggt ggcaataaac agatcgcccg cctgcacgcc aaagaacggt
    19201 ttcatccctt ccaccgcctg acgctgggcg tcattaaaca gtgtggcagc ttttgcctgc
    19261 gcattcggcc cttgcacggc aatcatggaa aggtcatcac gaacggtaat ttcgatgccg
    19321 aaaggttcag cgtgttgggt aatccaggag aggtcttttt cgcgggtggc ggagttaaca
    19381 acgaggcgga agaaatcttc agtaaagtag tagacgatga ggtcatctat cacaccgcca
    19441 gaggcattca acatccccga gtaaagggct ttgccgcttt tggtgagctt cgccacatcg
    19501 ttcgccagca gataacgcag aaactcccgg gtgcggctgc cgcggagatc gacgatggtc
    19561 atatgtgaca catcaaacat tccggcatcg gtacgtaccg catgatgttc gtcgatttgc
    19621 gaaccgtaat gcagcggcat catccagccg tggaaatcca ccatgcgagc gccgcaaagc
    19681 gtgtgttgtt cgtacaaagg agtctgttgt gccatcttgt cctcattgaa taagcggggc
    19741 tgacaacttt tcatggtgaa attatcacca cgaaacccag catcggagcc actcccggtc
    19801 cccaacgcaa tcgttctctt ttgcctgaac ttaccaccga aacagactgt taaccataag
    19861 gtaaaattga tcatcacatt agcttatggt taaaaaacgc caaaatcgcg acagaataaa
    19921 aaaccaaaaa acacaccagt ttctatataa agatgatgcc attagaaagc taatttgaat
    19981 aaggcaatat taagagctaa aaaaatgtca aaaaacacta aatcaaaaaa taatgacatt
    20041 agaaaatata atgcgaaaac ggaggtgaaa ttagtttatt tcaaatgagg aaaatctccc
    20101 ggcgaaaaaa ccgggagatg aaagtgtgat gggtatcaaa taaacagcag aagagaaatt
    20161 tttaacgcag ccattcaggc aaatcgttta atcccattgc ctggcggata agttgtggtt
    20221 taacgccagg aagcgtgtcg gccagtttca aaccaatatc acgcagcagt tttttcgccg
    20281 gattggtacc ggaaaacaga tcgcggaatc cctgcatacc agccagcatc agcgccgcac
    20341 tgtgcttgcg gctacgctca taccggcgca ggtaaatgta ctgcccgatg tctttcccct
    20401 gacgatgcaa ccgtttcagt tcagcaatca gctctgcagc atccataaag ccaagattta
    20461 ctccctgccc cgccagcggg tgaatggtat gcgcggcgtc gcccaccagc accagacggt
    20521 gcgcggcaaa ctggcgcgca taacgccccg tcagtgggaa cacctgacgc gcgctctcaa
    20581 ccttgcataa gcccaggcga ttatcaaaag cgatatttaa cgcgcgatta aattcatctt
    20641 cacttgcctg ctgcatccgc tgcgcttcct ctggcgacag tgaccagaca attgagcaaa
    20701 gatgcggatc gctaagcggt aaaaaggcca gaatgccttc gccatggaaa acctgccgcg
    20761 ccaccgcatc atgcggttct tccgtgcgaa tggtcgctac cagcgcgtga tgctgataat
    20821 cccagaaagt cagcggaata tctgctttgt tgcgcaacca ggaattagca ccgtccgcgc
    20881 caatcaccag acgcgccgtt aacatgctgc catctttcag cgtcaggaaa gtttcatttt
    20941 ctccccaggc gacctgctgt aattctgcgg gggccaacag agtgatatct gacgactgct
    21001 gcgctttgtt ccacagcgcg tagtgaatca ctgagttttc aacgatatgc ccaagatggc
    21061 tatagcccat gctttgatcg tcaaacgaaa tgtgaccaaa actgtctttg tcccacactt
    21121 ccataccgtg ataacaactg gccctacgag agagaatgtc ctgccagacg ccaagacggg
    21181 tgagtaattt ttcgctggcg gcattgatag ccgaaacgcg cagttgtggt ggtgcatccg
    21241 ccgccagagg ttccggtacg cgctgctcca gtacggcaac gcgtaagccg ctcccctgta
    21301 agccgcaggc aaccgccagc cccaccatgc cgccaccaac aatggctaca tcaacacttt
    21361 gcattgttta ttccttaaaa ccgccttcaa cgcgccaccc aaccgagggt gcgctgcgcc
    21421 agcacatcgc gtgccggggt gaataattcc atcgtcatca gtccgatgtt gcgcccgaca
    21481 accagcggtg cccaacggtt ggcaaaaaga tgtaccaggc tgtccgtgac gccgatggtt
    21541 gcttcgcgat cgctctgtcg acgctgctga tagcggcaca atatgccgta atcaccgatg
    21601 tcttctccgc gctcatgcgc ctgagtcagg gtttccgcaa ggctcatgac atctcgcata
    21661 ccgaggttaa acccttgccc ggcaatcggg tgcagagttt gcgccgcatt gcccaccagc
    21721 acggtacgat gggtaatagc tctggcggcg cgggttaacg ccagcggata agcactgcgt
    21781 ttaccggcgt gggtaatttt tccaagtcgc cagccaaagg ccgactggag ttcacggcaa
    21841 aacttctcgt cgctccacga cagcacctct tcgcgccgtt ccagtggatg acaccagacc
    21901 agcgaacagc gtccgtcaga catcggcaac atcgccagcg ggccatgttg cgtgaagcgt
    21961 tcaaaagcgc ggccttcgtg cgcaacggaa gtcgcaacgt tggcaatcac ggccagttgt
    22021 tcgtaaggct cctgctgcca gtcaacgccg cacgcggtgg ctaacgctga atgggtgcca
    22081 tcagctgcta ccagcacgcg gcccgtcagc gtctcgccac tctccagcgt cacttcaacg
    22141 tgactctgag tacgggcaac gttagccacg cgatcagggc aatgcagcgt tacgccaggt
    22201 gctttacgca gcaatgcaaa cagccgttgc ccgacattgt gcaattcgac aacctgtccc
    22261 agcgccgcca gttggtaatc ttctgcggcg agggtgacaa atccagcgtg accacgatcg
    22321 ctgacatgca cggtagtgat ggcggttgcg taatccgcca gagattgcca gacgccgatg
    22381 cgcgccagtt gctgacaggt ccccgcagcc agcgcaatag ctcgtccatc aaagcccgga
    22441 tgagcatgtg actctggcgc ggtcgcttca atcaaatgta ccggcagcgc cccgtgactt
    22501 aaccgggaaa tagccagcgc cagcgtcgcg cccgccatgc cgccaccgac gatgattacg
    22561 ctcattgctt tctcgcagct gccatcaacg cttcgatttc ttccggcttt ttcaccacgc
    22621 tggcggtgag gttttcgttg ccggtttcgg taatcacaat gtcgtcttca atacgaatgc
    22681 cgataccgcg atattgttct ggcacatccg catccggcgc gatatacaga ccaggctcta
    22741 cggtcagtac catccccggt tccagaatgc gcgagcgatc ctgaccataa acgccaacgt
    22801 catggacatc cagtcctaac cagtggctaa ggccatgcat aaagaaagga cgatgggcgt
    22861 tctgagcgat cagttcatca acatcacctt tcaggatgcc gagttttacc aggccgctaa
    22921 ccatgatgcg caccacttca ccagtgactt ccagaatgga agttcccgga cgatacaggc
    22981 gcaggctggt ttcgagagac tccagcacaa tgtcgtagat ttcacgctgg gcctgggtga
    23041 atttgccgtt gaccgggaag gtgcgggtaa tatcgcccgc gtaacctttg tattcacaac
    23101 ccgcgtcaat caacaccagg tcaccgtcgc gcagttcact ctcgttttcg gtgtagtgca
    23161 gaatgcagcc gttttcaccg ctgccgacaa tggtgttata ggacggatag cgcgcaccgt
    23221 ggcggttaaa ttcgtggtga atttcgcctt ccagatggta ctcgaacatt cccggacggc
    23281 atttttccat cgcccgtgta tgtgccatgg cggtgatttc tcccgcgcgg cggagtacgg
    23341 caatctcttc tggcgatttg aacaggcgca tttcatgaac aacaggacgc cagtcgatca
    23401 tcgttgccgg tgcggtgaga ttttgccgcg aacctttacg cagtttttcc agcgcactgt
    23461 tcatgatttc atcagcatat gcatattcgc cctgggcatg gtaaaccaca tccaggccgt
    23521 taagtagttg ataaagttgc tgattgattt cgctgaatgc cagtgcgcgg tcaacgccca
    23581 gtttctctgg cgcggcatcc tggcctaagc gacggccaaa ccagatctcc gccgtcaggt
    23641 cgcgaacgcg gttaaacaga acgctgtggt tatgagtgtc atcgctttta atcagcacca
    23701 gcaccgcttc caattcgtta aagccggtga agtaccagaa gtcactgttc tgacgatagg
    23761 ggtattcgct gtcggcgcta cgtgttactt ctggtgcagc aaaaatcagc gcggcgctgc
    23821 cgggttgcat ttgctccacc agggcctgac ggcgacgctg aaactcttgc cgggatatct
    23881 cactcataac actctcctta cgttttttgt ttttagtgta gagtcggttt ttgtacttct
    23941 ggcgcggtcg gttgcggatg agtaaaggtg tcgtggcata acagcgcggc aacacgaacg
    24001 tattcgatga tctcttcaag cgacatttca agctcttcct gatcttcgtc ttcgtcgtaa
    24061 cccagttgcg caatgttacg cagatcgtcg atagcttcac cggtttcgcc ggtcacttta
    24121 tccagcttcg gttgcgtaac gccaagacca agcaggaagt gattgaccca acctgccaat
    24181 gcatcagccc gatcgaaaac gctgacatca tcgccatcag gcagataaag ctgaaaaagg
    24241 aagccgtcat cctgcagggc atcgctggtg gcagagtgca ttttacgcag tgcctgtgcc
    24301 agctcatgac cgaaagccat gccttcgttc gtcaggtcgt gaagtagcgg cagccatgag
    24361 ctgtcatcgt taccgccaca tatcatccca ctgattaaac catgcatctc ggcaggggtc
    24421 agccccgtcc cttgttggtt cagatactgg ttcatttcgt tgtaaccagg catttcgttc
    24481 tgtatagaca taagcattcg tcgtcaaagg gaggaatatt catgatatgc taccactttg
    24541 ggccctggtg gaccagaaaa gggcttgtct cttctcatca gggtagctat agtgtcgccc
    24601 cttcgcagac catgggtcta aagacgaagg cagcgcagtc aatcagcagg aaggtggcat
    24661 gtctgcacaa cccgtcgata tccaaatttt tggccgttca ctgcgtgtga actgcccgcc
    24721 tgaccaaagg gatgcgttga atcaggcagc ggacgatctg aaccaacggt tgcaagatct
    24781 gaaagaacgc actagagtca caaatactga acagttggtc ttcattgccg cattgaatat
    24841 cagctatgag ttagcgcaag aaaaagcgaa gactcgtgac tacgcggcaa gtatggaaca
    24901 gcgtattcgg atgctgcagc agaccataga acaagcgtta cttgaacaag gtcgcatcac
    24961 cgaaaaaact aaccaaaact ttgaatgaca cttttcggtt tactgtggta gagtaaccgt
    25021 gaagacaaaa tttctctgag atgttcgcaa gcgggccagt cccctgagcc gatatttcat
    25081 accacaagaa tgtggcgctc cgcggttggt gagcatgctc ggtccgtccg agaagcctta
    25141 aaactgcgac gacacattca ccttgaacca agggttcaag ggttacagcc tgcggcggca
    25201 tctcggagat tcccttctta tctggcacca gccatgacgc aactaccaga actcccactg
    25261 acattatccc gacaagaaat ccgcaaaatg attcggcaac gtcgtcgtgc gttaacgccg
    25321 gaacaacagc aggaaatggg tcaacaagcc gctacccgga tgatgactta tcccccggtg
    25381 gtgatggcgc atacggtcgc tgtattcctc tcttttgatg gtgaactcga cacccagccg
    25441 ctaatagaac aactctggcg cgccggtaag cgcgtatatc ttccagtttt gcatcccttt
    25501 agtgccggta atttgctgtt cctgaattac catccgcaaa gcgaactggt gatgaacagg
    25561 ttgaagatcc atgagccaaa actggatgtg cgtgacgtgc tacccctttc ccgattagac
    25621 gtgctgatca caccgctggt cgcctttgat gagtacggtc agcgcctggg aatgggcggt
    25681 ggtttttatg atcggacctt acaaaactgg cagcactata aaacgcaacc ggtgggttat
    25741 gcgcatgatt gtcagttggt ggaaaaactc cccgttgaag agtgggatat ccctcttcct
    25801 gcggtggtta caccgtcgaa agtctgggag tggtaagggc gatacacctg catcgccctg
    25861 attgacatcg ttgattcttt aacctaattt agtgagtaag ggtaagggag gattgctcct
    25921 cccctgtgac tgactgttaa taagctggga aacttatgag taacagtaca atcagtatga
    25981 tggcaagtcg catcataacc cttctccttc aagccctcgc ttcggtgagg gctttaccgt
    26041 tacagcccca tgctgcccag ctatcgtaag ctctctttaa ataactacaa cgcattgatc
    26101 tacctttaaa tcttttcttc gagcgtgctc gaaaaaccca tgcaaaacag agataatttt
    26161 tgggaaaaag gatctattac ggcagtaaac gtgggcgatg aagtggctat caaaaagttc
    26221 ggttcagggc gatgcacccc gcatcgccct gattgacatc gttgattctt tgacctaatt
    26281 tagtgagtaa gggtaagaga ggatgactcc tcccctgaga ctgactgtta ataagctccg
    26341 aaacttatca gtaacagtac aatcagtatg atgacaagtc gcatcataac ccttctcctt
    26401 taagccctcg cttcggtgag ggctttaccg ttacagcccc atgctgtccc gccatcgtaa
    26461 atccccatta aataaacaca acgcattgat ctgagtttga tttattttct ggagcagact
    26521 cgcaaagtag aatgcgcaac gcggcaacgg tgtggagaag ggataaaaaa acgggcaagt
    26581 cagtgacctg cccgttaatt ttcagagaag gggaattagt acagcagacg ggcgcgaatg
    26641 gtacccggaa tagctttcat tgcctgcagc gctttttcgg caacgtcttc gtcagcttca
    26701 atatcaataa ctacataacc catctgggcg gaagtttgta gatattgcgc ggcgatgttg
    26761 acgccctgct cggcgaagat tttgttcagc gcagttagca cgcccggacg gttttcgtgg
    26821 atgtgcatca gacgacgacc accatgcagt ggcagcgaga cttccgggaa gttcaccgca
    26881 gagagcgttg agccattgtc agaatacttg atcaatttac ccgcaacttc caggccgata
    26941 ttctcctgcg cttcctgagt cgaaccgcca atgtgtggcg tcagaaggac gttgtcgaat
    27001 tcacacagtg gagaggtaaa tggatcgcta ttggtcgccg gttccgtcgg gaatacgtcg
    27061 attgccgccc ccgccagatg tttgctcgcc agcgcatcac acagcgccgg aatatccacc
    27121 acagtaccgc gcgaggcatt aatcagcagc gagccgggct tcattagcga aatctctttc
    27181 gcgcccatca tatttttggt ggacggattc tctggtacat gcagactcac cacatcgctc
    27241 atattcagca ggtcagaaag atgctgtacc tgagtggcgt tgcccagcgg cagtttgttt
    27301 tcaatatcat aaaagtaaac atacattccc agcgattcag ccagaatgcc caattgcgta
    27361 ccaatatgac cgtagccgat gatacccagc tttttgccgc gcgcttcaaa agaacccgcc
    27421 gccagtttgt tccacacgcc acggtgcgct ttagcattgg cttccggcac gccgcgcaat
    27481 agcagcagca gttcgccaat caccagctcc gcaacagagc gcgtatttga gaacggtgcg
    27541 ttaaataccg ggatcccgcg ctttgccgcc gcatccagat caacctggtt tgttccgata
    27601 cagaaacagc caatagcgac cagtttttct gcggcgttga tcacgtcttc agtcagatgg
    27661 gtacgggatc gcaggccgat gaagtgggca tcgcggatgg attcttttaa ttgttcatca
    27721 tccagcgcgc ctttgtgaaa ttcgatgttg gtgtaaccag ctgcacgaag gctttccagc
    27781 gccttttggt gcacgccttc taccagcaga aacttaatct tgtctttctc cagcgatacc
    27841 tttgccattt acccaatcct gtcttttgaa atgttgtgtg cggatttgca tccgcctttc
    27901 aacatatcaa aaaataatat tgcggcaata tgaacgtttg cgtcgcgatg ttgaggaaat
    27961 atcacgcaaa ggtatttctt ggttgaatat gctggtttag aggatttatt tagagcaatc
    28021 gacaatagcc aagtaaaaac gtgacacatg tcaccaaatt taatgaagag aattttttta
    28081 acgggggagg ttcccccgtc agatcatttc acaatggttt tgacaccgtc aggtgtgcca
    28141 atcagcgcaa cgtccgcgcc acggttagca aacaagccaa cagtcaccac gccaggaatc
    28201 gcatttatgg cgttttccat cgctatcggg tcaaggattt ccatgccgtg gacgtcgagg
    28261 atcacgttgc cgttatcggt caccacgccc tgacggtatt ccggacgacc gcccagtttc
    28321 accagctggc gcgccactgc actacgtgcc atcgggataa cttctactgg cagcgggaat
    28381 ttgcccagaa tatcaacctg cttggaagcg tcagcaatac agataaattt ttccgcaacc
    28441 gaagcaatga ttttttcacg ggtcagcgcc gcgccgccgc ctttgatcat ttgcatgtgg
    28501 ccgttgattt catctgcgcc atcaacgtag atgccaaggc tgtcgacttc gttgagatca
    28561 aaaacgtgaa tgccgaggct tttcagtttt tcagtggaag catctgaact ggaaacggcc
    28621 ccttcaatct ggcctttcat tgtaccgagc gcgtcaataa agtgtgcggc ggtggaacct
    28681 gtacctacac caacaatggt gccgggctga acatactgaa gtgccgccca tcctactgct
    28741 tttttcaatt catcctgcgt catgatcgtt tcgcctgtgg tatgaaattt cacacgcatt
    28801 atatacaaaa aaagcgattc agaccccgtt ggcaagccgc gtggttaact catccataaa
    28861 atatcgcgca atggcgggca tcccctttcg ccccgcaaat aaagcataca acggtctggg
    28921 tatgccgctc cacggtgcga acaggcgcac cagttccccc cgttcgcaag cccctgttta
    28981 caggcaaatt gaggcaataa cgccacccca ttcaacacaa ccagggcacg aactcgctga
    29041 gcgaaatgca ttagcgcaaa tccctgatct tttaagctgc gcgtttaacc acggatacaa
    29101 atgttacccg ccgacggctt cggtatatgc aacctgacac aaaattgtgt catagtgcag
    29161 gaaaaagcat ttaccaggag cagacaacag caatgaaacg cccggactac agaacattac
    29221 aggcactgga tgcggtgata cgtgaacgag gatttgagcg cgcggcacaa aagctgtgca
    29281 ttacacaatc agccgtctca cagcgcatta agcaactgga aaatatgttc gggcagccgc
    29341 tgttggtgcg taccgtaccg ccgcgcccga cggaacaagg gcaaaaactg ctggcactgc
    29401 tgcgccaggt ggagttgctg gaagaagagt ggctgggcga tgaacaaacc ggttcgactc
    29461 cgctgctgct ttcactggcg gtcaacgccg acagtctggc gacgtggttg cttccggcac
    29521 tggctcctgt gttggctgat tcgcctatcc gcctcaactt gcaggtagaa gatgaaaccc
    29581 gtactcagga acgtctgcgc cgcggcgaag tggtcggcgc ggtgagtatt caacatcagg
    29641 cgctgccgag ttgtcttgtc gataaacttg gtgcgctcga ctatctgttc gtcagctcaa
    29701 aaccctttgc cgaaaaatat ttccctaacg gcgtaacgcg ttcggcatta ctgaaagcac
    29761 ctgtggtcgc ctttgaccat cttgacgata tgcaccaggc ctttttgcag caaaacttcg
    29821 atctgcctcc aggcagcgtg ccctgccata tcgttaattc ttcagaagcg ttcgtgcaac
    29881 ttgctcgcca gggcaccacc tgctgtatga tcccgcacct gcaaatcgag aaagaactag
    29941 ccagcggtga actgattgac ttaacgccag ggctatttca acgccggatg ctctactggc
    30001 accgctttgc tcctgaaagc cgcatgatgc gtaaagtcac tgatgcgttg ctcgattatg
    30061 gtcacaaagt ccttcgtcag gattaatcca tcaaataatg cctgatagca catatcaggc
    30121 gttatcctca cttctttttg tattccttga atcacatcac aaaatagaca aatctcaggc
    30181 ggcaaaaaac gacgtctgaa tgcatttttt tgctggcgac aaacccacgt aaaaagctca
    30241 ccgtaagcgc aaataccctc attttgattg cgttttacgg agcaaataat gtctaacgag
    30301 caggagtggc aacagcttgc caacaaggaa ttgagccgtc gggagaaaac tgtcgactcg
    30361 ctggttcagc aaaccgcgga agggatcgcc atcaagcagc tgtataccga agccgatctc
    30421 gataatctgg aggtgacagg tacccttcct ggtttgccgc cctacgttcg tggcccgcgt
    30481 gccactatgt ataccgccca accgtggacc atccgtcagt atgctggttt ttcaacagca
    30541 aaagagtcca acgcttttta tcgccgtaac ctggccgccg ggcaaaaagg tctttccgtt
    30601 gcgtttgacc ttgccaccca ccgtggctac gactccgata acccacgcgt ggcgggtgac
    30661 gtcggcaaag cgggcgtcgc tatcgacacc gtggaagata tgaaagtcct gttcgaccag
    30721 atcccgctgg ataaaatgtc ggtttcgatg accatgaatg gcgcagtgct accagtactg
    30781 gcgttttata tcgtcgccgc agaagagcaa ggtgttacac ctgataaact gaccggcacc
    30841 attcaaaacg atattctcaa agagtacctc tgccgcaaca cctatattta cccaccaaaa
    30901 ccgtcaatgc gcattatcgc cgacatcatc gcctggtgtt ccggcaacat gccgcgattt
    30961 aataccatca gtatcagcgg ttaccacatg ggggaagcgg gtgccaactg cgtgcagcag
    31021 gtagcattta cgctcgccga tgggattgag tacatcaaag cagcaatctc tgccggactg
    31081 aaaattgatg acttcgctcc tcgcctgtcg ttcttcttcg gtatcggcat ggatctgttt
    31141 atgaacgtcg ccatgttgcg tgcggcacgt tatttatgga gcgaagcggt cagtggattt
    31201 ggcgcacagg atccgaaatc actggcgctg cgtacccact gccagacctc aggctggagc
    31261 ctgactgaac aggatccgta taacaacgtt atccgcacca ccattgaagc actggctgcg
    31321 acgctgggcg gtactcagtc actgcatacc aacgcctttg acgaagcgct tggtttgcct
    31381 accgatttct cagcacgcat tgcccgcaac acccagatca tcatccagga agaatcagaa
    31441 ctctgccgca ccgtcgatcc actggccgga tcctattaca ttgagtcgct gaccgatcaa
    31501 atcgtcaaac aagccagagc tattatccaa cagatcgacg aagccggtgg catggcgaaa
    31561 gcgatcgaag caggtctgcc aaaacgaatg atcgaagagg cctcagcgcg cgaacagtcg
    31621 ctgatcgacc aaggcaagcg tgtcatcgtt ggtgtcaaca agtacaaact ggatcacgaa
    31681 gacgaaaccg atgtacttga gatcgacaac gtgatggtgc gtaacgagca aattgcttcg
    31741 ctggaacgca ttcgcgccac ccgtgatgat gccgccgtaa ccgccgcgtt gaacgccctg
    31801 actcacgccg cacagcataa cgaaaacctg ctggctgccg ctgttaatgc cgctcgcgtt
    31861 cgcgccaccc tgggtgaaat ttccgatgcg ctggaagtcg ctttcgaccg ttatctggtg
    31921 ccaagccagt gtgttaccgg cgtgattgcg caaagctatc atcagtctga gaaatcggcc
    31981 tccgagttcg atgccattgt tgcgcaaacg gagcagttcc ttgccgacaa tggtcgtcgc
    32041 ccgcgcattc tgatcgctaa gatgggccag gatggacacg atcgcggcgc gaaagtgatc
    32101 gccagcgcct attccgatct cggtttcgac gtagatttaa gcccgatgtt ctctacacct
    32161 gaagagatcg cccgcctggc cgtagaaaac gacgttcacg tagtgggcgc atcctcactg
    32221 gctgccgatc ataaaacgct gatcccggaa ctggtcgaag cgctgaaaaa atggggacgc
    32281 gaagatatct gcgtggtcgc gggtggcgtc attccgccgc aggattacgc cttcctgcaa
    32341 gagcgcggcg tggcggcgat ttatggtcca ggtacaccta tgctcgacag tgtgcgcgac
    32401 gtactgaatc tgataagcca gcatcatgat taatgaagcc acgctggcag aaagtattcg
    32461 ccgcttacgt cagggtgagc gtgccacgct cgcccaggcc atgacgctgg tggaaagccg
    32521 tcacccacgt catcaggcgc taagtacaca gctgcttgac gccattatgc cgttctgtgg
    32581 taacgccctg cgactgggcg ttaccggcac acccggcgcg gggaaaagta cctttcttga
    32641 agcctttggc atgttgttga ttcgtgaggg attaaaggtt gctgttattg cggtcgatcc
    32701 cagcagcccg gtcactggcg gtagcattct cggggataaa acccgcatga atgacctggc
    32761 gcgtgccgag gcggcgttta ttcgcccggt accctcctcc ggtcatctgg gcggtgccag
    32821 tcagcgagca cgggaattaa tgctgttatg cgaagcggcg ggttatgacg tagtgattgt
    32881 cgaaacggtt ggcgtcgggc agtcggaaac ggaagtcgcc cgcatggtgg actgttttat
    32941 ctcgttgcaa attgccggtg gcggcgatga tctgcagggc attaaaaaag ggctgatgga
    33001 agtggctgat ctgctcgtta tcaacaaaga cgatggcgat aaccatacca atgtcgccat
    33061 tgcccggcat atgtacgaga gtgccctgca tattctgcga cgtaaatacg acgaatggca
    33121 gccacgggtt ctgacttgta gcgcactgga aaaacgtgga atcgatgaga tctggcacgc
    33181 catcatcgac ttcaaaaccg cgctaactgc cagtggtcgt ttacaacaag tgcggcaaca
    33241 acaatcggtg gaatggctgc gtaagcagac cgaagaagaa gtactgaatc acctgttcgc
    33301 gaatgaagat ttcgatcgct attaccgcca gacgctttta gcggtcaaaa acaatacgct
    33361 ctcaccgcgc accggcctgc ggcagctcag tgaatttatc cagacgcaat attttgatta
    33421 aaggaatttt tatgtcttat cagtatgtga acgttgccac tatcaacaaa gtggcggtca
    33481 ttgagtttaa ctatggacga aaacttaatg ccttaagtaa agtctttatt gatgatctta
    33541 tgcaggcgtt aagcgatctc aaccggccgg aaattcgctg tatcattttg cgggcgccaa
    33601 gtggatccaa agtcttctcc gcaggtcacg atattcacga actgccgtct ggcggtcgcg
    33661 atccgctctc ctatgatgat ccattgcgtc aaatcacccg catgatccaa aaattcccga
    33721 aaccgatcat ttcgatggtg gaaggtagtg tttggggtgg cgcatttgaa atgatcatga
    33781 gttccgatct gatcatcgcc gccagtacct caaccttctc aatgatgcct gtaaacctcg
    33841 gcgtcccgta taacctggtc ggcattcaca acctgacccg cgacgcgggc ttccacattg
    33901 tcaaagagct gatttttacc gcttcgccaa tcaccgccca gcgcgcgttg gctgtcggca
    33961 tcctcaacca tgttgtggaa gtggaagaac tggaagattt caccttacaa atggcgcacc
    34021 acatctctga gaaagcgccg ttagccattg ctgttatcaa agaagagctg cgtgtactgg
    34081 gcgaagcgca caccatgaac tccgatgaat ttgaacgtat tcaggggatg cgccgcgcgg
    34141 tttatgacag cgaagattac caggaaggga tgaacgcttt cctcgaaaaa cgtaaaccta
    34201 atttcgttgg tcattaatcc ctgcgaacga aggagtaaaa atggaaactc agtggacaag
    34261 gatgaccgcc gatgaagcgg cagaaattat ccagcataac gacatggtgg catttagcgg
    34321 ctttacccca gcgggttcgc cgaaagcctt accgaccgcg attgcccgca gagctaacga
    34381 acagcatgag gccaaaaagc cgtatcaaat tcgcctgctg acgggggcgt caatcagcgc
    34441 cgccgctgac gatgtacttt ctgacgccga tgctgtttcc tggcgtgcgc catatcaaac
    34501 atcgtccggt ttacgtaaaa agatcaatca gggcgcggtg agtttcgttg acctgcattt
    34561 gagcgaagtg gcgcaaatgg tcaattacgg tttcttcggc gacattgatg ttgccgtcat
    34621 cgaagcatcg gcactggcac cggatggtcg agtctggtta accagcggga tcggtaatgc
    34681 gccgacatgg ctgctgcggg cgaagaaagt gatcattgaa cttaatcact atcacgatcc
    34741 gcgcgttgca gaactggcgg atattgtgat tcctggcgca ccaccgcggc gcaatagcgt
    34801 gtcgatcttc catgcaatgg atcgcgtcgg tacccgctat gtgcaaatcg atccgaaaaa
    34861 gattgtcgcc gtcgtggaaa ccaacttgcc cgacgccggt aatatgctgg ataagcaaaa
    34921 tcccatgtgc cagcagattg ccgataacgt ggtcacgttc ttattgcagg aaatggcgca
    34981 tgggcgtatt ccgccggaat ttctgccgct gcaaagtggc gtgggcaata tcaataatgc
    35041 ggtaatggcg cgtctggggg aaaacccgga aattcctccg ttcatgatgt attcggaagt
    35101 gctacaggaa tcggtggtgc atttactgga aaccggcaaa atcagcgggg ccagcgcctc
    35161 cagcctgaca atctcggccg attccctgcg caagatttac gacaatatgg attactttgc
    35221 cagccgcatt gtgttgcgtc cgcaggagat ttccaataac ccggaaatca tccgtcgtct
    35281 gggcgtcatc gctctgaacg tcggcctgga gtttgatatt tacgggcatg caaattcaac
    35341 gcacgtagcc ggggtcgatc tgatgaacgg catcggcggc agcggtgatt ttgaacgcaa
    35401 cgcgtatctg tcgatcttta tggctccgtc gattgctaaa gaaggcaaga tctcaaccgt
    35461 cgtgccaatg tgcagccatg ttgatcacag cgaacacagc gtcaaagtga tcatcaccga
    35521 acaagggatc gccgatctgc gcggtctttc cccgcttcaa cgcgcccgca ctatcattga
    35581 taattgtgca catcctatgt atcgggatta tctgcatcgc tatctggaaa atgcgcccgg
    35641 cggacatatt caccacgatc ttagccacgt cttcgactta caccgtaatt taattgcaac
    35701 cggctcgatg ctgggttaat ggtttgtccc gtcacgatct tcagcaatat gctgaagcat
    35761 cgtggcggta taacgatgat ttctgattga ataaagatat tcctgcatat acatcggcct
    35821 gtgcggcgca tcaaaatatt tcagcgttgc cggattcacc agtcgccagg caaaatgcgg
    35881 gatcaccgtc agaaaatgtc cgcgctccac ggcgctgatt ttggccataa aactgtaggg
    35941 acgataaata atggtgggat tgatcccaca aggtcgcata ttcgcatcaa tcattgcttc
    36001 gaagttagcc cggttttgaa agcgcatttg tagccagggc agctcctgaa gcagctcttg
    36061 ctcttgacgc tcttcataac ggcgagaaac gagaaagccc aatcgcagcg gtggtaattc
    36121 actgatagtc aggttttcca gttcctgaac tcgtgcagat acatgctgtg gggagataat
    36181 aaaatctaac tgacggtcaa aaagattatc gataacgccg ttttcgctga actcaactgg
    36241 ctgggcggtc acgccttcat acttatctcc gagactaatc aactgatcga aaataattgt
    36301 cggataagta ttatctatgc caatcacaat t
//
LOCUS       DADMKF010000026        36106 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.026, whole genome shotgun sequence.
ACCESSION   DADMKF010000026 DADMKF010000000
VERSION     DADMKF010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36106
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.026"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            246..581
                     /locus_tag="JNC24_001165"
     CDS             246..581
                     /locus_tag="JNC24_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004856597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phnA family protein"
                     /protein_id="HAY9157975.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            739..1182
                     /gene="yjdN"
                     /locus_tag="JNC24_001166"
                     /pseudo
     CDS             739..1182
                     /gene="yjdN"
                     /locus_tag="JNC24_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418531.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
     gene            1315..2103
                     /gene="phnC"
                     /locus_tag="JNC24_001167"
     CDS             1315..2103
                     /gene="phnC"
                     /locus_tag="JNC24_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HAY9157976.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVALNIHHGEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTREQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQQFDNERFDHLYRSINRIEENAKAA"
     gene            2128..3144
                     /gene="phnD"
                     /locus_tag="JNC24_001168"
     CDS             2128..3144
                     /gene="phnD"
                     /locus_tag="JNC24_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY9157977.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLSPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLIPGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEIQGVKSNNGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            3270..4049
                     /gene="phnE"
                     /locus_tag="JNC24_001169"
     CDS             3270..4049
                     /gene="phnE"
                     /locus_tag="JNC24_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HAY9157978.1"
                     /translation="MQTITIAPPKRSWFSLLSWAIVLAVLVVSWQGAEMAPLTLIKDG
                     GNMATFAADFFPPDFSQWQDYLTEMAVTLQIAVWGTALAVVLSIPFGLMSAENLVPWW
                     VYQPVRRLMDACRAINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAVEAI
                     EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIG
                     VTLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLRKHFI"
     gene            4070..4795
                     /gene="phnF"
                     /locus_tag="JNC24_001170"
     CDS             4070..4795
                     /gene="phnF"
                     /locus_tag="JNC24_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HAY9157979.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAAR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGIGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVADALGITEGENVIHLRTLRRVNGVALCLIDHYFADLTLWPTLQ
                     RFDSGSLHDFLREQTGIALRRSQTRISARRAQAKECQRLEIPNMSPLLCVRTLNHRDG
                     ESSPAEYSVSLTRADMIEFTMEH"
     gene            4796..5248
                     /gene="phnG"
                     /locus_tag="JNC24_001171"
     CDS             4796..5248
                     /gene="phnG"
                     /locus_tag="JNC24_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HAY9157980.1"
                     /translation="MHADTATRQHWMSVLAHSQPAELAARLNALNITADYEVIRTAET
                     GLVQIQARMGGTGERFFAGDATLTRAAVRLTDGTLGYSWVLGRDKQHAERCALIDALM
                     QQSRHFQNLSETLIAPLDADRMARIAARQAEVNASRVDFFTMVRGDNA"
     gene            5245..5829
                     /gene="phnH"
                     /locus_tag="JNC24_001172"
     CDS             5245..5829
                     /gene="phnH"
                     /locus_tag="JNC24_001172"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HAY9157981.1"
                     /translation="MTLETAFMLPVQDAQHSFRRLLKAMSEPGVIVALYQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLATPLSNDIVNQSLRFHTNAPLVSQPEQATFAVTDEAIS
                     SEQLNALSTDTAVAPEAGATLILQVASLSGGRMLRLTGAGIAEERMIAPQLPECILHE
                     LTERPHPFPLGIDLILTCGERLLAIPRTTHVEVC"
     gene            5829..6893
                     /gene="phnI"
                     /locus_tag="JNC24_001173"
     CDS             5829..6893
                     /gene="phnI"
                     /locus_tag="JNC24_001173"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HAY9157982.1"
                     /translation="MYVAVKGGEKAIDAAHALQESRRRGDTDLPELSVAQIEQQLNLA
                     VDRVMTEGGIADRELAALALKQASGDNVEAIFLLRAYRTTLTKLAVSEPLDTTEMRLE
                     RRISAVYKDIPGGQLLGPTYDYTHRLLDFTLLANGEAPTLTTADSEQQPSPHVFSLLA
                     RQGLAKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDVSIVPEELGFAVNVGELLMTECEMVNGFIDPPDEPPHFTRGYGLV
                     FGMSERKAMAMALVDRALQAPEYGEHATGPAQDEEFVLAHADNVEAAGFVSHLKLPHY
                     VDFQAELELLKRLQQEQNHG"
     gene            6886..7731
                     /gene="phnJ"
                     /locus_tag="JNC24_001174"
     CDS             6886..7731
                     /gene="phnJ"
                     /locus_tag="JNC24_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HAY9157983.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTECTDDAT
                     LIQTRHRIPETPLTEDQIIIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWDEPCAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQS
                     EAKSQ"
     gene            7728..8486
                     /gene="phnK"
                     /locus_tag="JNC24_001175"
     CDS             7728..8486
                     /gene="phnK"
                     /locus_tag="JNC24_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HAY9157984.1"
                     /translation="MNQPLLSVNNLTHLYAPGKGFSDVSFDLWPGEVLGIVGESGSGK
                     TTLLKSISARLTPQQGEIHYENRSLYAMSEADRRRLLRTEWGVVHQHPLDGLRRQVSA
                     GGNIGERLMATGARHYGDIRATAQKWLEEVEIPANRIDDLPTTFSGGMQQRLQIARNL
                     VTHPKLVFMDEPTGGLDVSVQARLLDLLRGLVVELNLAVVIVTHDLGVARLLADRLLV
                     MKQGQVVESGLTDRVLDDPHHPYTQLLVSSVLQN"
     gene            8597..9277
                     /gene="phnL"
                     /locus_tag="JNC24_001176"
     CDS             8597..9277
                     /gene="phnL"
                     /locus_tag="JNC24_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HAY9157985.1"
                     /translation="MINVQNVSKTFILHQQNGVRLPVLNRASLIVNAGECVVLHGHSG
                     SGKSTLLRSLYANYLPDEGQIQIKHGDEWVDLVTAPARKVVEIRKTTVGWVSQFLRVI
                     PRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWHLAPSTFSGGEQQRVNI
                     ARGFIVDYPILLLDEPTASLDAKNSAAVVELIHEAKARGAAIVGIFHDEAVRNDVADR
                     LHPMEASS"
     gene            9274..10410
                     /gene="phnM"
                     /locus_tag="JNC24_001177"
     CDS             9274..10410
                     /gene="phnM"
                     /locus_tag="JNC24_001177"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HAY9157986.1"
                     /translation="MIINNVKLVLENEVVHGSLEVQDGEIRAFAESQSRLPEAMDGEG
                     GWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSHDALMVASGITTVLDAVAIGDVR
                     DGGDRLVNLEKMINAIEETQKRGVNRAEHRLHLRCELPHHTTLPLFEKLVQREPVTLV
                     SLMDHSPGQRQFANREKYREYYQGKYSLTDAQMQQYEEEQLALAARWSQPNRESIAAL
                     CRARKIALASHDDATHAHVAESHQLGSVIAEFPTTFEAAEASRKHGMNVLMGAPNIVR
                     GGSHSGNVAASELAQLGLLDILSSDYYPASLLDAAFRVADDESNRFTLPQAVKLVTKN
                     PAQALNLQDRGVIGEGKRADLVLAHRKGNHIHIDHVWRQGKRVF"
     gene            10410..10967
                     /gene="phnN"
                     /locus_tag="JNC24_001178"
     CDS             10410..10967
                     /gene="phnN"
                     /locus_tag="JNC24_001178"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HAY9157987.1"
                     /translation="MMGKLIWLMGPSGSGKDSLLAELRLREQTQLLVAHRYITRDASA
                     GSENHIALSEQEFFTRAGQNLLALSWHANGLYYGVGVEIDLWLHAGFDVLVNGSRAHL
                     PQARARYQSALLPVCLQVSPEILRQRLENRGRENASEINARLARAARYTPQDCHTLNN
                     DGSLRQSVDTLLTLIHQKEKHHACL"
     gene            10954..11388
                     /gene="phnO"
                     /locus_tag="JNC24_001179"
     CDS             10954..11388
                     /gene="phnO"
                     /locus_tag="JNC24_001179"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
                     /protein_id="HAY9157988.1"
                     /translation="MPACELRPATQYDTDAVYALICELKQAEFDHHAFRVGFSANLRD
                     PNMRYHLALLDGEVVGMIGLHLQFHLHHVNWIGEIQELVVMPQARGLNVGSKLLAWAE
                     EEARQAGAEMTELSTNVKRHDAHRFYLREGYEQSHFRFTKAL"
     gene            11390..12148
                     /gene="phnP"
                     /locus_tag="JNC24_001180"
     CDS             11390..12148
                     /gene="phnP"
                     /locus_tag="JNC24_001180"
                     /EC_number="3.1.4.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
                     /protein_id="HAY9157989.1"
                     /translation="MSLTLTLTGTGGAQGVPAWGCECAACDRARRSPQYRRQPCSGVV
                     KFNEAITLIDAGLHDLTDRWSPGSFQQFLLTHYHMDHVQGLFPLRWGVGDPIPIYGPP
                     DEQGCDDLFKHPGLLDFSHTVEPFVVFDLQGLQVTPLPLNHSKLTFGYLLETAHSRVA
                     WLSDTAGLPEKTLKFLRNNQPQVMVMDCSHPPRADAPRNHCDLNTVLALNQVIRSPRV
                     ILTHISHQFDAWLMENALPSGFEVGFDGMEIGVA"
     gene            complement(12295..12624)
                     /gene="yjdP"
                     /locus_tag="JNC24_001181"
     CDS             complement(12295..12624)
                     /gene="yjdP"
                     /locus_tag="JNC24_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjdP"
                     /protein_id="HAY9157990.1"
                     /translation="MKRFPLFLLFTLLTLSTVPAQADIIDDTIGNIQQAINDAYNPDR
                     GRDYEDSRDDGWQREVSDDRRRQYDDRRRQFEDRRRQLDDRQRQLDQERRQLEDEERR
                     MEDEYGR"
     gene            complement(12693..13010)
                     /locus_tag="JNC24_001182"
     CDS             complement(12693..13010)
                     /locus_tag="JNC24_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase"
                     /protein_id="HAY9157991.1"
                     /translation="MQPVFLFKKRTGAMGGHVAGTAGFCVAVPAFYSWSMSEVIDKDT
                     RSSERTDYPHYASEVALAFGSRVVGLELAKMIVDAWLGAQYEGGRHQQRVEAITAIEQ
                     RRN"
     gene            13263..15248
                     /gene="yjcS"
                     /locus_tag="JNC24_001183"
     CDS             13263..15248
                     /gene="yjcS"
                     /locus_tag="JNC24_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase YjcS"
                     /protein_id="HAY9157992.1"
                     /translation="MNNSRLFRLSRIVIALTAASGMMVNTANAKEEAKAATQYTQQVN
                     QNYAKSLPFSDRQDFDDAQRGFIAPLLDEGILRDANGKPYYRGEDYKFDINAPAPETV
                     NPSLWRQSQLNGISGLFKVTDRMYQVRGQDISNITFIEGDTGIIVIDPLVTPPSAKAA
                     LDLYFQNRPQKPIVAVIYTHSHTDHYGGVKGIISEADVKSGKVQVIAPAGFMDEAISE
                     NVLAGNIMSRRALYSYGLLLAHNPQGNIGNGLGVTLASGYPSIIAPNKTITKTGEKMI
                     IDGLEFDFLMTPGSEAPAEMHFYIPALKALCTAENATHTLHNFYTLRGAKTRDTSKWT
                     EYLNETLDMWGNDAEVLFMPHTWPVWGNKHINDYIGKYRDTIKYIHDQTLHLANQGYT
                     MNEIGDMIKLPPALANNWASRGYYGSVSHNARAVYNFYLGYYDGNPANLHPYGQVEMG
                     KRYVQALGGSARVINLAQEANKQGDYRWSAELLKQVIAANPGDQVAKNLQANNFEQLG
                     YQAESATWRGFYLTGAKELREGVHKFSHGTIGSPDTIRGMSVEMLFDFMSVRLDSAKA
                     AGKNISLNFNMSNGDNLNLTLNDSVLNYRKTLQSQADASFYISREDLHAVLTGQAKMA
                     DLVKAKKAKIIGNGAKLEEIIACLDNFDLWVNIVTPN"
     gene            15457..15732
                     /locus_tag="JNC24_001184"
     CDS             15457..15732
                     /locus_tag="JNC24_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9157993.1"
                     /translation="MPTVLSRMAMQLKKTAWIIPVFMVSGCSLSPAIPVIGAYYPSWF
                     FCAIASLILMLITRRVIQRANINLAFVGIIYTALFALYAMLFWLAFF"
     gene            15751..16782
                     /gene="mdtN"
                     /locus_tag="JNC24_001185"
     CDS             15751..16782
                     /gene="mdtN"
                     /locus_tag="JNC24_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HAY9157994.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSINATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMATVRAPFDGR
                     VISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            16782..18833
                     /gene="mdtO"
                     /locus_tag="JNC24_001186"
     CDS             16782..18833
                     /gene="mdtO"
                     /locus_tag="JNC24_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HAY9157995.1"
                     /translation="MSALNSLPLPMVRLLAFFHEELSERRPGRVPQIVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGRMFLMRTHRLGLVFFAVAIVAIYGQTFPAMLDYPEVVVRLTLWCI
                     VVGLYPTLLMTLIGVLWFPSRAISQMHQALNDRLDDAISHLTDSLAPLPETRIEREAL
                     ALQKLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKL
                     ASEINKLQHAVAEGQCWQSDWRISESEAVAARECNLENICQTLLQLGQMNPNTPPTPA
                     AKPPSMVADAFTNPDYIRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVSNPNVG
                     SSYQKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERS
                     SYIGTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAVIYTFVWPESEART
                     LPQKLAGALGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLD
                     SEERALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAGQFADALEKYAAGL
                     ATALSRSPQITLEETPASQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGAT
                     Q"
     gene            18830..20296
                     /gene="mdtP"
                     /locus_tag="JNC24_001187"
     CDS             18830..20296
                     /gene="mdtP"
                     /locus_tag="JNC24_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HAY9157996.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDAIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAERVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAAPVVEKK"
     gene            20494..22641
                     /gene="fdhF"
                     /locus_tag="JNC24_001188"
     CDS             20494..22641
                     /gene="fdhF"
                     /locus_tag="JNC24_001188"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:20911..20913,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HAY9157997.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVSWDEALNYVTERLSAIKEKYGPDA
                     IQTTGSSRGTGNETNYVMQKFARAVIGTNNVDCCARVUHGPSVAGLHQSVGNGAMSNA
                     INEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIVCDPRKIETARIADMHIAL
                     KNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKIVEGYTPESVEDITGVSAS
                     EIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLAMLTGNLGKPHAGVNPVRG
                     QNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVESLPAHTGYRISELPHRAAH
                     GEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFMTKTASAADVILPSTSWGE
                     HEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMGYPMHYNNTQEIWDELRHL
                     CPDFYGATYEKMGELGFIQWPCRDTSDADQGTSYLFKEKFDTPNGLAQFFTCDWVAPI
                     DKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPGYAQINTEDAKRLGIEDEA
                     LVWVHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTENLSPITKTPEYKYCA
                     VRVEPIADQRAAEQYVIDEYNKLKTRLREAALA"
     gene            22735..23424
                     /locus_tag="JNC24_001189"
     CDS             22735..23424
                     /locus_tag="JNC24_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HAY9157998.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(23503..24816)
                     /gene="gltP"
                     /locus_tag="JNC24_001190"
     CDS             complement(23503..24816)
                     /gene="gltP"
                     /locus_tag="JNC24_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HAY9157999.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVPGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(25158..25754)
                     /gene="nrfG"
                     /locus_tag="JNC24_001191"
     CDS             complement(25158..25754)
                     /gene="nrfG"
                     /locus_tag="JNC24_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HAY9158000.1"
                     /translation="MKQPQIPVKMLTTLTILMVFLCVGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTCAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            complement(25751..26134)
                     /gene="nrfF"
                     /locus_tag="JNC24_001192"
     CDS             complement(25751..26134)
                     /gene="nrfF"
                     /locus_tag="JNC24_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HAY9158001.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVSEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            complement(26127..27845)
                     /locus_tag="JNC24_001193"
     CDS             complement(26127..27845)
                     /locus_tag="JNC24_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HAY9158002.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWEFKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQTDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDAASARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFCH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRAQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQAKGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTGADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GKKRDE"
     gene            complement(27865..28821)
                     /gene="nrfD"
                     /locus_tag="JNC24_001194"
     CDS             complement(27865..28821)
                     /gene="nrfD"
                     /locus_tag="JNC24_001194"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HAY9158003.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPILPVLFLFSGISSGAAVALIAMAIRQRSNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            complement(28818..29489)
                     /gene="nrfC"
                     /locus_tag="JNC24_001195"
     CDS             complement(28818..29489)
                     /gene="nrfC"
                     /locus_tag="JNC24_001195"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HAY9158004.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            complement(29486..30052)
                     /gene="nrfB"
                     /locus_tag="JNC24_001196"
     CDS             complement(29486..30052)
                     /gene="nrfB"
                     /locus_tag="JNC24_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HAY9158005.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAAQASDDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            complement(30097..31533)
                     /gene="nrfA"
                     /locus_tag="JNC24_001197"
     CDS             complement(30097..31533)
                     /gene="nrfA"
                     /locus_tag="JNC24_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HAY9158006.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHPDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            31927..33885
                     /gene="acs"
                     /locus_tag="JNC24_001198"
     CDS             31927..33885
                     /gene="acs"
                     /locus_tag="JNC24_001198"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006816721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HAY9158007.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSINVPDTFWGEQGK
                     ILDWIKPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLELGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHS
                     VIFGGFSPEAVAGRIIDSNSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSVEHVV
                     VLKRTGGKIDWQEGRDLWWHDLVEQASDQHQAEKMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDG
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            34017..34330
                     /locus_tag="JNC24_001199"
                     /pseudo
     CDS             34017..34330
                     /locus_tag="JNC24_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
     gene            34327..35976
                     /gene="actP"
                     /locus_tag="JNC24_001200"
     CDS             34327..35976
                     /gene="actP"
                     /locus_tag="JNC24_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HAY9158008.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKLVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTFADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMMGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSITVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
BASE COUNT         8404 a        10186 c         9712 g         7804 t
ORIGIN      
        1 gtcagaaaga taaaaatatc atgtgaataa aaaaaagaac aagtagagca ttaacattat
       61 cttaaataat aaatagaggc aaaaagatta ttttcttttt gcgtttcctt tcaaatgaaa
      121 acgatcgtcg tctaaaatca gcagtacccc cgacaaactc agggattttg tgtataattg
      181 cggccttttt cggcaatctg ccgttttttg gcgcttttgc cctgctgact tttgaggaaa
      241 tccacatgtc attaccacac tgcccaaaat gcaactccga atacacttac gaagataacg
      301 gcatgtacat ctgcccggaa tgtgcctacg aatggaacga cgcagaacct gcacaggaaa
      361 gcgacgagct gatcgttaaa gatgctaacg gcaatctgct ggctgacggc gacagcgtta
      421 ccatcattaa agatctgaag gtgaaaggta gctcttcgat gctgaaaatt ggcaccaaag
      481 tgaaaaacat ccgcctggtt gaaggcgacc ataacatcga ttgcaaaatc gacggttttg
      541 gtccgatgaa actgaaatct gagtttgtga aaaagaactg attgtattgt gatcggtaag
      601 ccggataagg cgctcgcgcc gcatccggca acgatgccga ctgcctgatg cgacgcttgc
      661 gcgtcttatc aggcagcctc tcatctgtat aaatttcgaa ctacacttaa ttggcttctc
      721 ttaactgagg tcaccatcat gccgttaagt ccctacctct cttttgccgg taactgtgcc
      781 gacgcaattg cttattatca acgtacgttg ggcgctgaac tgctctataa aatcagcttc
      841 ggcgaaatgc caaaatcagc gcaggacagc gccgagaact gcccttccgg aatgcaattt
      901 cccgataccg ccatcgccca cgccaatgtg cgcattgccg gaagcgacat catgatgagc
      961 gatgccatgc cgtcaggaaa agccagctac tccggcttta cgctggtgct cgattcgcaa
     1021 caggtcgaag aaggaaaacg ctggtttgac aatcttgccg ctaacggaaa aatcgaaatg
     1081 gcctggcagg aaactttcta ggcgcatggc tttggcaaag tcaccgataa atttggcgta
     1141 ccgtggatga ttaatgtcgt caaacaacaa ccaacgcaat aacccgccgg gaggcccgcc
     1201 ctcccgcact gtcatcgaat tcccgttaac tcttcatctg ttagtcactt ttaattaacc
     1261 aaatcgtcac aataatccgc cacgatggag ccactttttt agggaggctg catcatgcaa
     1321 acgattatcc gtgtcgagaa gctcgccaaa accttcaatc agcatcaggc gctgcatgcg
     1381 gttgctctga acattcatca cggtgaaatg gtggctctgc ttgggccgtc gggttccggc
     1441 aaatccaccc ttttacgtca cttaagcggt ttgattaccg gcgataaatc cgccggcagc
     1501 catatcgagc tgctgggccg cacagtccag cgcgaaggcc gtctggcgcg cgatatccgc
     1561 aaaagccgcg ccaacaccgg ctacatcttc caacaattca acctggtgaa ccgcctgagc
     1621 gtactggaga acgtgctgat tggcgcgctc ggcagcacgc cgttctggcg cacctgtttt
     1681 agctggttta cccgcgagca gaaacaacgc gcgttacagg cgctgacccg cgttggcatg
     1741 gtgcattttg cccatcaacg cgtttccacc ctctccggcg gacagcagca gcgtgtggcg
     1801 attgcccgcg cgctgatgca gcaggcgaag gtgattctgg ccgatgaacc catcgcctcg
     1861 ctggacccgg aatccgcccg catcgtgatg gacaccctgc gcgacatcaa tcagaacgac
     1921 ggcatcaccg tggtcgtcac gctgcatcag gtggattacg ccctgcgcta ctgcgaacgc
     1981 atcgtcgccc tgcgccaggg gcacgttttc tacgacggca gcagccaaca gtttgataac
     2041 gaacgttttg accatctcta ccgcagcatt aatcgcatcg aagagaacgc gaaagctgcc
     2101 tgacatcccc atcattgagg aaaacgaatg aacgctaaga taattgcctc gctggccttc
     2161 accagcatgt tcagcctcag caccctgtta agcccggcac acgccgaaga gcaggaaaag
     2221 gcgctgaatt tcggcattat ttcaacggaa tcacagcaaa acctgaaacc gcaatggacg
     2281 ccattcttac aggatatgga gaagaagctg ggcgtgaagg tgaacgcctt ctttgcccca
     2341 gactacgcag gcattatcca gggaatgcgc ttcaataaag tggatatcgc ctggtacggc
     2401 aacctgtcgg caatggaagc ggtggatcgc gccaacggcc aggtcttcgc ccagacggtc
     2461 gcggcggatg gatcgccagg ttactggagc gtgttgatcg tcaacaaaga tagtccgatc
     2521 aacaacctga acgatctgct ggcgaagcgg aaagatctca ccttcggcaa tggcgatcct
     2581 aactccacct ctggcttcct cgtccccggt tactacgtct ttgccaaaaa caatatctcc
     2641 gccagcgact ttaagcgcac cgtcaacgcc gggcatgaaa ccaacgcgct ggccgtcgcc
     2701 aacaagcagg tggatgttgc caccaacaac accgaaaacc tcgacaagct gaaaacctcc
     2761 gcgccagaga agctgaaaga actgaaggtg atctggaagt cgccgctgat cccaggcgat
     2821 ccgatcgtct ggcgcaagaa tctctccgaa accaccaaag acaagatcta cgacttcttt
     2881 atgaactacg gaaaaacgcc ggaagagaaa gcggtgctgg aacgcctggg ctgggcaccg
     2941 tttcgcgcct ccagcgacct gcaactggtg ccgattcgcc agctcgcact gtttaaagag
     3001 attcagggcg taaaaagcaa taacggactg aatgagcagg acaagctggc gaaaaccacc
     3061 gagattcagg cgcagctgga tgacctggac cgcctgaaca acgcgttaag cgcgatgagt
     3121 tcggtgagta aagcggtgca gtaatgtgct cgatcggtcc cctctccctt gagggagagg
     3181 gttagggtga gggggaaatg cgcacgctga tgccctcacc ccggccctct cccacaggga
     3241 gagggagaaa acattaaacg gagccaaaca tgcaaaccat caccatcgcc ccacccaagc
     3301 gcagctggtt ctcgcttctg agctgggcca ttgttctcgc cgtgttggtc gtctcgtggc
     3361 agggcgcgga aatggccccg cttacgctga tcaaagacgg cggcaacatg gcgaccttcg
     3421 ccgccgactt cttcccgccc gatttcagcc agtggcagga ttacctcacc gaaatggccg
     3481 tcacgctgca aatcgccgtc tggggcaccg cgctggcagt ggttctctcc atccccttcg
     3541 gcctgatgag cgccgaaaac ctggtgccgt ggtgggttta ccagcccgtt cgccgcctga
     3601 tggacgcctg ccgcgccatt aacgaaatgg tcttcgccat gctgttcgtg gtcgccgtcg
     3661 gtctcggacc gttcgctggc gtgctggcgc tatttattca caccaccggc gtgctctcga
     3721 aactgctttc cgaagcggtg gaagcgattg aacccggccc ggtggaaggc attcgcgcca
     3781 ccggcgccaa caagctggaa gagatccttt acggcgtgct gccacaggta atgccgctgc
     3841 tgatctccta ctccctctat cgcttcgaat ccaacgtccg ctcggcgacc gtcgtcggca
     3901 tggtcggtgc aggcgggatc ggcgtcaccc tgtgggaagc gattcgcggt ttccagttcc
     3961 aacaaacctg cgccctgatg gtgcttatca tcgtcacggt cagcctgctg gatttcctct
     4021 ctcaacggtt gcgtaagcac tttatctgat aagtgaggca ttgatatcta tgcacttgtc
     4081 tacacatccg accagctacc caacacgcta tcaggagata gccgcaaaac ttgagcagga
     4141 gcttcgtcaa cactatcgct gcggcgacta tcttcccgcc gagcagcaac tggcggcgcg
     4201 ctttgaggta aaccgccaca ccctgcgccg cgccatcgac caactggtgg aaaaaggctg
     4261 ggtacagcgc cgtcagggca tcggcgtgct ggtgctgatg cgcccgttcg attacccgct
     4321 caacgcccag gcgcgtttta gccagaacct gctggaccag ggcagccatc ccaccagcga
     4381 aaaactgctg tcggtattgc gccccgcgtc cggtcacgtt gctgacgcgc tggggattac
     4441 cgagggtgag aacgttatcc atttgcgcac cctgcgtcgc gtcaacggcg tcgcgctctg
     4501 tttaatcgac cactactttg cggacctcac cctctggccg acactgcaac gtttcgacag
     4561 cggttcgctg cacgattttc tgcgcgagca aaccggaatt gcgctgcgcc gcagccagac
     4621 gcggatcagc gcccgccgcg cccaggccaa agagtgccag cgccttgaaa tcccgaatat
     4681 gtcgccgctg ctgtgcgtgc gcacccttaa ccaccgtgac ggtgaaagca gcccggcgga
     4741 gtactccgtc agcctgacgc gcgccgacat gattgaattc actatggagc actgaatgca
     4801 cgcagatacc gcgacccgcc agcactggat gtccgtactg gcgcacagcc aaccggctga
     4861 actggcagcc cgcctgaacg cgctgaacat caccgccgac tatgaagtga tccgcactgc
     4921 cgaaactggc ctggtgcaga ttcaggcgcg aatgggcggt accggcgaac gcttttttgc
     4981 cggcgacgcc acgctgaccc gtgccgccgt acgcctgact gacggcaccc tcggctacag
     5041 ctgggtgctg gggcgtgata aacagcacgc cgaacgctgc gcgctgattg atgcgctgat
     5101 gcagcaatct cgccactttc aaaacttatc agaaaccctt attgccccgc tggacgctga
     5161 ccgtatggca cgcattgccg cacgccaggc cgaagtgaac gccagccggg tcgacttctt
     5221 tacgatggtt cgcggagaca acgcatgacc ctggaaaccg cttttatgct tcccgtgcag
     5281 gatgcccagc acagttttcg tcgcctgtta aaggccatga gcgagccggg cgtgattgtc
     5341 gccctgtatc agctcaaacg cggctggcag ccgctgaata tcgccaccac cagcgtgctg
     5401 ctgacgctgg ccgataacga cacgccggtg tggcttgcca cgccattaag taacgatatc
     5461 gtcaatcaga gcctgcgttt tcataccaac gcgccgctgg tcagccagcc ggaacaggcg
     5521 accttcgcgg tgacggatga ggcgatttcc agcgaacagc tcaacgccct ttccaccgac
     5581 accgccgttg cgccggaagc gggcgcgacg ctgattttac aggtcgccag cctgagcggc
     5641 ggacgcatgt tgcgtctcac cggcgcgggc attgccgaag aacgaatgat cgctccgcag
     5701 ctgccagagt gcattctgca cgaactcacc gagcgcccgc acccgttccc gctcggcatc
     5761 gacctaatcc tgacctgcgg cgagcgcctg ctggctatcc cgcgaaccac tcatgtggag
     5821 gtgtgctgat gtacgttgcc gtgaaagggg gcgaaaaggc gatcgacgcc gcccacgccc
     5881 tgcaagagag ccgacgccgg ggcgataccg atttgcccga actgagcgtc gcccagattg
     5941 aacagcagct taacctcgcg gtggatcgcg tgatgacaga aggcggcatt gccgaccgcg
     6001 aactggcggc gctggcgctg aaacaggcca gcggcgataa cgtcgaagcg attttcctgc
     6061 tacgcgccta ccgcaccacg ctgacgaaac tggcggtaag cgagccgctc gacaccacgg
     6121 agatgcgtct cgaacggcgt atttcagcgg tttataaaga tattcccggc ggccagctgc
     6181 ttggcccaac ctacgactac acccatcgcc tgctcgattt cactctgctg gcaaacggcg
     6241 aagcgccgac gctgaccacc gccgacagcg aacaacagcc gtcgccgcac gttttcagcc
     6301 tgctggcgcg tcaggggctg gcgaagtttg aagaggatag cggagcacag ccggatgaca
     6361 tcacccgcac gccgccggtt tacccctgtt cacgttcttc ccgtttgcag cagttaatgc
     6421 gcggcgacga aggctatttg ctggcgctgg cttactccac ccagcgcggt tacgggcgca
     6481 accacccgtt cgcgggcgag atccgcagtg gttacatcga cgtgtcgatt gtgccggaag
     6541 agctgggatt tgcggtaaac gtcggcgaac tgctgatgac cgagtgtgaa atggtcaacg
     6601 gttttatcga cccgccggat gagccgccgc acttcacgcg cggctacggg ctggtattcg
     6661 gcatgagcga gcgcaaagcg atggcaatgg cgctggttga ccgtgcgttg caagccccgg
     6721 aatacggcga gcacgcgaca ggcccggcgc aggatgaaga gttcgtgctg gcgcatgccg
     6781 acaacgtcga agccgcaggc tttgtctcgc acctcaaact cccgcactac gtcgatttcc
     6841 aggccgaact ggagctactc aaacgtctgc aacaggagca gaaccatggc taatctgagc
     6901 ggctacaact ttgcctacct cgacgagcag accaaacgca tgatccgccg cgccatctta
     6961 aaagcggtgg cgatccccgg ttatcaggta ccgtttggcg ggcgcgagat gccgatgccg
     7021 tacggctggg gaactggtgg tatccagctc accgccagcg tgattggcga aagcgacgtg
     7081 ttgaaggtga ttgaccaggg tgcggatgac accaccaacg ccgtgtcgat tcgcaacttc
     7141 tttaagcgcg tgaccggggt aaacaccact gaatgtacgg acgatgcgac gcttatccag
     7201 acgcgtcacc gcatccccga aacgccgctg accgaagatc agatcattat cttccaggtg
     7261 ccaatcccgg aaccgctgcg ctttatcgag ccgcgcgaaa cggagacccg cactatgcac
     7321 gcgctggagg agtacggcgt gatgcaggtg aaactgtatg aagatatcgc ccgcttcggt
     7381 catatcgcca ccacctatgc ttatccggtg aaggtgaacg ggcgctacgt gatggacccg
     7441 tcgccgatcc cgaaattcga taacccgaaa atggacatga tgcccgccct gcagctgttc
     7501 ggcgcagggc gcgagaagcg catctatgcg gtgccgccgt ttacccgcgt ggaaagtctc
     7561 gatttcgacg atcacccgtt caccgttcag cagtgggatg agccatgcgc catctgcgga
     7621 tcgacccaca gctatcttga tgaagtggtg ctggatgacg ccggaaaccg catgtttgtc
     7681 tgctccgata ccgattattg ccgccaacag agcgaggcga aaagccaatg aatcaaccgt
     7741 tactttcggt caataacctg acccaccttt acgcgccggg caaaggcttt agcgatgtct
     7801 cttttgattt atggccgggg gaagtgctgg gcattgtcgg ggaatccggc tccgggaaga
     7861 ccacgctgct gaagtcgatc tccgcgcgtc tgacgccgca gcagggggag attcactatg
     7921 aaaaccgttc cctctatgca atgagcgagg ccgaccgccg tcgcctgctg cgcaccgaat
     7981 ggggcgtggt gcatcagcat ccgctcgacg gcctgcgccg ccaggtgtcg gcaggcggca
     8041 atatcggcga gcggctgatg gcgaccgggg cgcgacatta cggcgatatt cgcgctaccg
     8101 cgcagaagtg gctggaagag gtggagattc ccgccaaccg tatcgacgac ctgccgacca
     8161 ccttttccgg cggtatgcag cagcgtttgc agattgcccg caacctggtg acgcatccga
     8221 agctggtgtt tatggatgaa ccgaccggcg ggctggatgt gtcggtgcag gcccgcctgc
     8281 tcgacctgct gcgcggactg gtggtggagc tgaacctcgc ggtagtgatt gtcacccacg
     8341 atttaggcgt cgcccgcctg ctggcggacc gtttgctggt gatgaagcag gggcaagtgg
     8401 tggagagtgg gttaaccgac cgcgtgctcg acgacccgca tcatccgtat acacagctgc
     8461 tggtgtcatc ggttttgcag aattgagccg gtgccggatg cggcgtaaac gccttatccg
     8521 gcctacaaat gcgctccccg taggtcggat aagacgcgtc agcgtcgcat ccgacacccg
     8581 aaccacgagg cgaaaaatga ttaacgtaca aaacgtcagt aaaaccttca tcctgcacca
     8641 gcaaaacggc gtgcgcctgc ccgtcctcaa ccgcgcctcg ctcatcgtca acgcgggcga
     8701 atgcgtggtg ctccacggcc attccggcag cggaaaatca accctgctac gctcgctgta
     8761 cgccaactat ctgcccgacg aagggcaaat ccagatcaaa cacggtgacg aatgggtcga
     8821 tctggtcacc gcgccagcgc gcaaagtggt ggagatccgc aaaaccaccg ttggctgggt
     8881 gagccagttt ctgcgcgtca tcccgcgtat ttcggcgctg gaagtggtga tgcagccgct
     8941 actcgatacc ggcgtaccgc gtgaagcctg cgccgctaaa gccgcgcgtc tgctcacccg
     9001 cctgaacgtg ccggaacgcc tgtggcatct ggcgccatcg accttctccg gcggcgaaca
     9061 gcagcgcgtc aacatcgccc gcggctttat cgtcgactac cccattctgc tgctcgacga
     9121 acctaccgcc tcgctggacg ccaaaaacag cgccgccgta gtcgagctga ttcacgaagc
     9181 caaagcccgt ggcgcggcca tcgtcggcat cttccacgac gaagccgtac gtaatgacgt
     9241 tgccgaccgc ctgcacccaa tggaagcctc ttcatgatta tcaataacgt caaattggtg
     9301 ctggaaaacg aagtggtaca cggttcactg gaggtgcagg acggcgaaat ccgcgccttt
     9361 gccgaaagcc agagccgcct gccggaggcg atggacggcg aaggcggctg gctgctgccg
     9421 gggctgattg agctgcatac cgataatctg gataaattct tcaccccgcg cccgaaagtt
     9481 gactggcctg cccactcggc gatgagcagc cacgacgcgc tgatggtggc gagcggcatc
     9541 accaccgtac tggatgccgt ggcaattggc gacgtgcgcg acggcggcga tcggctggtg
     9601 aatctggaga agatgatcaa cgccatcgaa gagacgcaga aacgcggcgt caaccgcgcc
     9661 gagcaccgtc tgcatctgcg ctgcgaactg ccgcatcaca ccacgctgcc gctgtttgaa
     9721 aaactggtgc agcgcgagcc ggtgacgctg gtgtcgctga tggaccactc gccgggccag
     9781 cgccagttcg ccaatcgcga gaagtatcgc gaatattatc agggcaaata ctccctcact
     9841 gatgcgcaga tgcagcagta cgaagaagag caactggcgc tcgccgcacg ctggtcgcag
     9901 ccgaatcgcg aatccatcgc cgccctgtgc cgcgcgcgaa aaattgcgct tgccagccac
     9961 gatgacgcca cccacgccca cgttgctgaa tctcaccagc ttggcagcgt gatcgccgaa
    10021 tttcccacca cgttcgaagc ggcggaagcc tcgcgcaagc atggcatgaa cgtgctgatg
    10081 ggcgcgccga atattgtgcg cggcggctcg cactccggca acgtggcggc cagtgaactg
    10141 gcgcagcttg gcctgctgga tatcctctct tccgactact accccgccag cctgctcgat
    10201 gcggcatttc gcgtcgccga tgacgagagc aaccgcttta cgctgccgca ggcggtgaag
    10261 ctggtgacta aaaatccagc gcaggcgctt aatctccagg atcgcggggt gattggcgag
    10321 ggcaaacgcg cggacttggt gctggcgcat cgcaagggca atcacattca tatcgaccac
    10381 gtctggcgtc agggtaaaag ggtgttctga tgatgggaaa actgatttgg ttaatggggc
    10441 cgtccggctc cgggaaagat agcctgctgg cggaactccg cctgcgggaa caaactcagc
    10501 tactggtggc gcatcgctac atcacgcgcg acgccagcgc cggaagcgaa aaccatatcg
    10561 ctctgagcga gcaggagttt tttacccgcg cggggcagaa cctactggcc ttaagctggc
    10621 acgctaacgg tctgtattat ggcgtcggcg tcgagatcga cctctggctg cacgccggat
    10681 tcgacgtgct ggtcaacggc tcacgcgccc atctgccgca ggcgcgggcg cgctatcaat
    10741 cggcgctgct gcccgtctgt ttacaggttt cgccggagat cctccgccag cgcctggaaa
    10801 accgtggccg tgaaaacgcc agtgaaatta acgcccgcct ggcgcgcgcc gcccgctata
    10861 ctccacagga ttgccatacg ctcaacaatg acggcagcct gcgccagtcg gtcgacacgc
    10921 tgctgacgct gattcatcag aaggagaaac accatgcctg cttgtgagct tcgcccggcc
    10981 acgcagtatg ataccgacgc ggtttacgcg ctgatttgtg agctaaaaca ggcggagttt
    11041 gaccaccacg catttcgcgt gggctttagc gccaatctgc gcgacccgaa catgcgctat
    11101 catctggcgc tgctggatgg cgaggttgtc ggcatgatcg gcctgcattt gcagtttcat
    11161 ctgcatcatg tcaactggat cggcgaaatt caggaactgg tagtgatgcc gcaggcgcgc
    11221 ggcctgaacg tcggtagcaa gttgctggcg tgggcagaag aagaagcccg ccaggccggg
    11281 gccgaaatga ccgagctttc gaccaacgtg aagcgccacg acgcgcaccg tttctatctg
    11341 cgagaaggct acgagcagag tcacttccgc ttcaccaagg cgctgtaaca tgagtctgac
    11401 cctcacgctc accggcaccg gcggcgcaca gggcgttccg gcatggggct gcgagtgcgc
    11461 ggcctgcgac cgagcgcggc gctcgccgca gtatcgccgc caaccgtgca gcggcgtagt
    11521 gaagtttaac gaggcgatca ccctgatcga cgccgggctg cacgatctca ccgatcgctg
    11581 gtctcccgga tcgttccagc agtttttgct gacgcattat catatggatc acgtccaggg
    11641 gctgtttccg ctgcgctggg gcgtgggcga tccgatcccg atctacggcc cgccggatga
    11701 acagggctgc gacgatctgt ttaaacatcc cgggctgctt gatttcagcc acacggtgga
    11761 accgtttgtg gtgtttgatt tgcaggggtt gcaggtcacg ccgctgccgc ttaaccactc
    11821 aaaactcact ttcggctatt tactggaaac ggcgcacagc cgggtggcgt ggctgtctga
    11881 caccgctggc ttgccggaaa aaacgctgaa atttttacgc aataatcagc cgcaggtaat
    11941 ggtgatggat tgcagtcacc cgccgcgcgc ggatgcaccg cgtaatcact gtgatttaaa
    12001 taccgtgctt gcgctgaatc aggttatccg ctcgccacgg gtgattctga cccatatcag
    12061 ccaccagttt gatgcgtggc tgatggaaaa cgcactaccg tcagggtttg aggtggggtt
    12121 tgatgggatg gagattgggg tggcgtgatg agagggaatg tgcgcgctgg ccccctcacc
    12181 ctaaccctct ccccagaggg gcgaggggac cgattgtgct caatattgaa tattgcgctc
    12241 gttttctccc tctccccatt ggggtgaggg gcgatgcctg ctccataccc aacctcatcg
    12301 cccatactca tcttccattc tccgctcttc atcctccagt tgccgacgct cctgatcaag
    12361 ctggcgctgg cgatcgtcca gctgcctgcg gcgatcttca aactggcggc ggcggtcgtc
    12421 atattgtctg cgccgatcgt cgctcacttc acgctgccag ccgtcgtcgc gcgaatcttc
    12481 atagtctcgc ccacggtcag ggttataagc gtcattaatc gcctgctgaa tattaccaat
    12541 agtatcgtcg ataatatcag cctgggccgg aacggtggac agcgtgagca gggtgaataa
    12601 aagaaatagc ggaaagcgtt tcattagcca acctcaaaaa gaaactctat ccacattagt
    12661 cattactcat ccatgcaagt agtggatgaa tctcaatttc tccgctgctc tattgccgta
    12721 atcgcctcca cgcgttgttg atgacgaccg ccttcgtact gtgcgcccag ccacgcatcc
    12781 acaatcattt ttgccagttc gaggccaacc actcgtgaac caaaagccag tgcaacttca
    12841 ctggcataat gcggataatc agtgcgctct gacgacctgg tgtctttatc aatcacctca
    12901 ctcattgacc atgaataaaa ggctggcaca gcaacgcaga atcctgcggt gccagcgaca
    12961 tgaccgccca ttgctccggt acgtttttta aaaagaaaca ccggttgcat tgataaattg
    13021 tgctcagtat ttgccgaagt acaatatata taataaatat ataaaacacc tcatgttatt
    13081 tacagagata ataaattcgc gtaattttcc ctcttcagca caaatgccta ataaaatgga
    13141 tcgttcagct cacacttcca cttattaaag tcgcaaattc ccaattatga taaccccagc
    13201 tttaaatgac gtattttgca tggattacat atttacgaca taaatgataa atggagattt
    13261 acatgaataa ctctcggtta ttccgtttga gcagaattgt tattgcgtta actgccgcca
    13321 gcggcatgat ggtaaatacc gctaacgcga aagaggaagc gaaagccgcc actcaatata
    13381 cccaacaggt taatcagaat tacgccaaat cattaccgtt tagcgatcgt caggattttg
    13441 acgacgctca gcgtggattt atcgctccgc tgctggatga aggtattctg cgcgatgcta
    13501 atggcaaacc atattatcgc ggggaagatt ataaatttga tatcaatgcg cccgcgcccg
    13561 aaaccgttaa ccctagcctg tggcgtcagt cgcagttaaa cggtatttcc ggcctgttta
    13621 aagtcaccga cagaatgtac caggttcgcg gtcaggatat ctccaacatc acctttatcg
    13681 aaggtgatac cgggattatt gtcatcgacc cgctggtgac gccaccgagc gcaaaagccg
    13741 cccttgacct ttacttccaa aatcgcccgc aaaaaccgat tgttgcggtt atttataccc
    13801 acagccatac cgaccactat ggcggcgtga aaggcattat ctctgaagct gatgtgaaat
    13861 ccggtaaagt gcaggttatc gcccctgctg gctttatgga cgaagccatc agcgaaaacg
    13921 tactggcggg taatattatg agccgccgtg cactttactc ctacggcctg ctgctggcgc
    13981 ataaccctca gggtaacatc ggcaatggtc ttggcgtaac gctggcatcg ggctacccga
    14041 gcatcatcgc accgaacaaa accatcacca aaaccggtga gaagatgatt atcgacggcc
    14101 tggagtttga cttcctgatg accccaggta gcgaagcacc agccgaaatg cacttctata
    14161 ttccggccct gaaagcgctg tgtaccgccg agaacgccac gcataccctg cacaacttct
    14221 acactctgcg cggcgcgaaa acccgcgaca ccagcaagtg gaccgagtat ctgaacgaaa
    14281 cgctggatat gtggggtaac gacgcggaag tcctgtttat gccgcacacc tggccggtct
    14341 ggggcaataa gcatatcaat gattatattg gtaaatatcg cgatactatc aagtacattc
    14401 acgaccagac cctgcacctg gcgaaccagg gctacaccat gaatgaaatc ggcgacatga
    14461 ttaaactgcc gcctgcactt gccaataact gggccagccg tggctattac ggttctgtca
    14521 gccacaacgc ccgcgcggtg tataacttct atcttggcta ttacgacggt aacccggcta
    14581 acctgcatcc gtatggtcag gtggagatgg gtaaacgtta cgtgcaggcg ctgggcggtt
    14641 ctgcccgtgt catcaacctg gcgcaagaag cgaacaagca aggtgattac cgctggtcgg
    14701 cagaactgct gaaacaggtg attgccgcca acccgggtga ccaggtcgcg aagaatctgc
    14761 aagcgaataa ctttgaacag ctgggctatc aggccgagtc cgccacctgg cgcggtttct
    14821 acctgaccgg cgcgaaagag ctgcgcgaag gggtgcataa gttcagccac ggcaccatcg
    14881 gttccccgga caccattcgc gggatgtcgg tcgaaatgct gttcgacttt atgtccgttc
    14941 gcctcgatag cgcgaaagcc gcgggtaaaa atatcagcct gaacttcaat atgagcaatg
    15001 gcgataacct caacctgacg ctgaacgata gcgtgcttaa ctaccgtaaa acactgcaat
    15061 cccaagctga cgcctctttc tacatcagcc gtgaagatct gcacgccgtg ctgaccgggc
    15121 aagccaaaat ggcggatctg gtaaaagcga agaaagccaa aattattggc aatggcgcga
    15181 aactggaaga aattatcgcc tgtctggata atttcgattt gtgggtgaat atcgtaaccc
    15241 caaattaatg tcactcgctt ttgggggcgc tgaagccccc aaaagcattt attttaaaaa
    15301 tatatacagc caattaataa ataaaatata taagttatga atgcggtgac tgaataaagc
    15361 tatttttatt catcgagaaa atagccatac tgacgccttc aatggatatt gatattcact
    15421 acagatattg ttcgcagcag acaggatgta tttaatatgc caacagttct ctctcgcatg
    15481 gcgatgcaac tcaaaaaaac agcctggata attcccgtct tcatggtttc gggatgctca
    15541 ttatctccgg caatcccggt gatcggtgct tattatccca gctggttttt ctgcgccatt
    15601 gccagcctta ttttgatgct catcacgagg cgagttattc agcgggcaaa tatcaatctg
    15661 gcatttgtcg gaattattta taccgccctt tttgctctct acgccatgct gttctggctg
    15721 gcatttttct aatctatttg agacgcgctc atggaaagta cgccgaaaaa agctcctcgc
    15781 agtaaattcc ctgctctgtt agtggttgca ttggcgctgg ttgcccttgt tttcgttatc
    15841 tggcgcgtag acagtgcgcc atcaactaat gacgcttacg cgtcagcaga taccattgat
    15901 gtggtgccgg aagtcagcgg ccgcattgta gaactggcgg tcaccgacaa ccaggcagtc
    15961 aaacagggcg atttgctgtt ccgcattgac ccgcgcccgt acgaagccaa tctggcgaaa
    16021 gctgaagcct ccctcgcggc gctggataag caaattatgc tcacccagcg tagcgttgat
    16081 gcgcaacagt ttggtgccga ctcgattaat gccacggtag aaaaagcccg tgccgccgcg
    16141 aaacaggcca cagatacatt acgccgtacc gagccattac tgaaagaggg ttttgtctca
    16201 gcggaagacg ttgaccgtgc aagaactgcg cagcgcgccg cagaagccga tcttaatgcc
    16261 gtattgttac aggcgcagtc agccgccagc gccgtcagcg gcgtcgatgc gctggtggcg
    16321 caacgtgcgg cggtcgaagc ggatattgcc ctgaccaaac tgcatctgga aatggcgacc
    16381 gttcgcgcgc cgtttgatgg ccgggtcatt tccctcaaaa cctccgtcgg gcaatttgct
    16441 tctgccatgc gccctatttt taccctaatc gacactcgtc actggtacgt gatcgccaac
    16501 ttccgcgaaa ccgatctgaa aaatattcgc tcaggtacac ccgcaacgat tcgcctgatg
    16561 agtgacagcg gcaaaacctt cgagggtaaa gtggattcga ttggctacgg cgtgctaccg
    16621 gatgacggcg gcctggtgct gggcggcctg ccgaaagttt ctcgctctat taactgggtc
    16681 cgcgttgccc agcgttttcc ggtcaaaatc atggtcgata aacctgaccc ggaaatgttc
    16741 cgcatcggcg cttcggcagt cgctaatctt gagccgcaat aatgagcgcg ctcaactccc
    16801 tgccattacc gatggtcagg ctgctggcgt tctttcatga agagttaagc gagcggcgac
    16861 ccggccgcgt gccgcagatc gtgcaactct gggtaggctg cctgctggtg attctgatct
    16921 cgatgacttt tgagatccct tttgtggcgt tatcgctggc agtgctgttt tacggtattc
    16981 agtcgaacgc gttttacacc aaatttgtgg cgatcttgtt tgtggttgcc acggtgctgg
    17041 agatcggcag cctgtttttg atctacaaat ggtcatacgg cgaaccgttg atccgattga
    17101 tcatcgccgg gccgatcctg atgggccgca tgtttttgat gcgcacccat cggctgggac
    17161 tggtcttttt cgctgtcgcc attgtcgcca tttacgggca aaccttcccc gccatgctcg
    17221 actatccgga agtggtcgtg cgcttaacgc tgtggtgtat cgttgttggc ctctatccaa
    17281 ccttgctgat gacgttaatc ggcgtgctgt ggtttcccag tcgtgccatt tcgcaaatgc
    17341 atcaggcgct taatgatcgg cttgatgatg ccattagtca cctgacagac agcctcgcac
    17401 cgctacccga aacgcggatt gaaagagagg cgctggcgct gcaaaaactg aatgtctttt
    17461 gtctcgcgga cgatgccaac tggcgaactc aaagcgcatg gtggcaaagc tgcgtggcaa
    17521 cggtaaccta catttactcg acgctgaatc gctacgatcc cacctctttt gctgattctc
    17581 aggcaattat tgaatttcgg caaaaattag cctcagaaat caacaagctg cagcatgccg
    17641 ttgctgaagg tcagtgctgg caaagcgact ggcggatcag tgaaagtgaa gcggtggcgg
    17701 cacgggaatg taacctggag aatatctgcc agacgttgtt acaactgggc cagatgaacc
    17761 cgaatacgcc gccaacgccc gccgccaaac cgccatcaat ggtcgccgat gcttttacca
    17821 atccagacta tatacgctac gcggtaaaaa cattgctcgc ctgtttgatc tgttacacct
    17881 tttacagcgg cgtggactgg gaaggcattc acacctgtat gctgacctgc gtgatcgtct
    17941 ctaatccaaa tgtcggttcg tcgtaccaga agatggtgct gcgttttggc ggggcctttt
    18001 gcggcgcgat tctggcgctg ttattcacgc tactggtcat gccctggctg gacaatattg
    18061 tcgaattgct gtttgtgctg gcaccgattt tcctgttggg cgcatggatt gccaccagct
    18121 ctgaacgctc ttcttatatc ggcacacaga tggtggtcac cttcgcgctc gccacgctcg
    18181 aaaacgtttt tggcccagtg tacgacctgg tggaaattcg cgatcgcgcc ctgggtatca
    18241 tcattggtac cgtggtgtcc gcggtgattt acacctttgt ctggcctgaa agtgaagcgc
    18301 gcacgctgcc gcaaaaactg gctggcgcgc tgggtatgtt aagtaaagta atgcggatcc
    18361 cacgccagca ggaagtcacg gctctgcgca cttatctgca aattcgtatc ggtctgcatg
    18421 cggcgtttaa tgcctgtgaa gagatgtgcc aacgcgtggc gctggagcgt caactggaca
    18481 gcgaagaacg cgccttactg attgaacgtt cgcaaacggt tattcgtcag ggccgcgata
    18541 tccttcacgc ctgggatgcc acctggaatt cggcgcaggc gctggataac gcactacagc
    18601 cggacagagc aggtcagttt gccgacgccc tggaaaaata cgctgccggt ctggcaaccg
    18661 cactcagccg ttctcctcaa ataacgcttg aagagacacc cgcctcgcag gccatcctgc
    18721 ccaccttatt aaaacaggag caacacgtct gccagctttt cgcccgcttg ccagactgga
    18781 cagccccggc attaacgccc gccacggaac aggcacaagg agccacgcaa tgatcaatcg
    18841 tcaactttca cgtctgctgt tgtgcagcat tctcggcagc acgacgctga tttccggctg
    18901 tgccttggta cgcaaggatt ctgcgcctca tcaacagctc aaaccggaac aaatcaaact
    18961 ggccgatgat attcatcttg ccagctccgg ctggccgcag gcgcagtggt ggaaacaact
    19021 caatgacccg cagctggatg cgctgatcca acggacgcta agtggttcac acaccctcgc
    19081 cgaagcgaaa ctgcgggaag aaaaagcgca gtcccaggcc gatttgttag atgccggttc
    19141 acaattacag gtcgcagcgt tagggatgct caaccgccaa cgcgtctcgg cgaacggctt
    19201 tttaagccct tatgcgatgg atgcgcccgc actgggtatg gacgggccgt actatacgga
    19261 agccacagta ggtttgtttg ccggactgga tcttgatttg tggggtgtgc atcgctcagc
    19321 ggttgccgcc gccattggcg cgcataatgc cgcgctggca gaaaccgcag cagtagagct
    19381 atcgctgacc acgggcgtag cgcagcttta ttacagtatg caggccagct atcagatgct
    19441 cgatctgtta gaacaaactc gcgatgtgat tgattacgcg gtgaaagcgc accagagtaa
    19501 agtggcgcac ggtctggaag cacaagtgcc tttccacggc gcgcgggcac agattctggc
    19561 ggtcgataaa caaattgctg ccgtcaaagg gcaaatcacc gaaacgcgag aatctctgcg
    19621 tgcattgatt ggcgcggggg ccagcgatat gccggagatc aaaccggtgg cattaccacg
    19681 agtccagacc ggcattccgg cgacactctc ttatgagttg ctcgccagac gcccggatct
    19741 gcaagccatg cgctggtatg ttcaggcgtc attagatcag gtggattccg cgcgggcgct
    19801 gttctacccg agctttgata tcaaagcgtt tttcggcctt gatgccatcc acctggatac
    19861 cttattcaaa aaaaccagtc gccagttcaa cttcatcccg ggtctgaaat tgccgctgtt
    19921 tgacggtgga cggttgaatg ccaatctcga aggcacgcgc gccgccagca acatgatgat
    19981 tgaacgttac aaccagtcag tactgaacgc ggtgcgtgac gttgccgtca acggcacgcg
    20041 tctgcaaacg ctcaacgacg agcgagaaat gcaggctgaa cgcgtggaag caacgcgctt
    20101 cacccagcgc gctgccgagg ccgcctatca gcgcggctta accagccgct tacaggccac
    20161 tgaagcccgg ttgccagtac ttgccgaaga gatgtcatta ctgatgctgg acagccgccg
    20221 ggtgatccaa agcattcagt tgatgaaatc gctgggcggc gggtatcagg cggctcccgt
    20281 cgtcgagaaa aaataaaatg tctgccgcgt gatggctgtc acgcggtatt tcgtttcgtc
    20341 acgtcaaaac tgacgacagc ctgtttttcg tcagagtttt gaataaatag tgcccgtaat
    20401 atcagggaat gaccccaaat aaaatgtggc ataaaagatg catactgtag tcgagagcgc
    20461 gtatgcgtga tttgattaac tggagcgaga ccgatgaaaa aagtcgtcac ggtttgcccc
    20521 tattgcgcat caggttgcaa aatcaacctg gtcgtcgata acggcaaaat cgtccgggcg
    20581 gaggcagcgc aggggaaaac caaccagggt accctgtgtc tgaagggtta ttatggctgg
    20641 gatttcatta acgataccca gatcctgacc ccgcgcctga aaacccccat gatccgtcgc
    20701 cagcgtggcg gcaaactcga acctgtttcc tgggatgagg cactgaatta cgttaccgag
    20761 cgcctgagcg ccatcaaaga gaagtacggt ccggatgcca tccagacgac cggctcctcg
    20821 cgtggtacgg gtaacgaaac caactatgta atgcaaaaat ttgcgcgcgc cgttattggt
    20881 accaataacg ttgactgctg cgctcgtgtc tgacacggcc catcggttgc aggtctgcac
    20941 caatcggtcg gtaatggcgc aatgagcaat gctattaacg aaattgataa taccgattta
    21001 gtgttcgttt tcgggtacaa cccggctgat tcccacccaa tcgtggcgaa tcacgtaatt
    21061 aacgctaaac gtaacggggc gaaaattatc gtctgcgatc cgcgcaaaat tgaaaccgcg
    21121 cgcattgctg acatgcacat tgcactgaaa aacggctcga acatcgcgct gttgaatgcg
    21181 atgggccatg tcattattga agaaaatctg tacgacaaag cgttcgtcgc ttcccgtaca
    21241 gaaggctttg aagagtatcg taaaatcgtt gaaggctaca cgccggagtc ggttgaagat
    21301 atcaccggcg tcagcgccag tgagattcgt caggcggcac ggatgtatgc ccaggcgaaa
    21361 agcgccgcca tcctgtgggg catgggtgta acccagttct accagggcgt ggaaaccgtg
    21421 cgttctctga ccagcctcgc gatgctgact ggtaacctcg gtaagccgca tgcgggtgta
    21481 aacccggttc gtggtcagaa caacgtgcag ggtgcctgcg atatgggcgc gctgccggat
    21541 acgtatccgg gataccagta cgtgaaagat ccggctaacc gcgagaaatt cgccaaagcc
    21601 tggggcgtgg aaagcctgcc tgctcatacc ggttatcgca tcagcgagct gccgcaccgc
    21661 gcagcgcatg gcgaagtgcg tgccgcgtac attatgggcg aagatccgct gcaaactgac
    21721 gcggagctgt cggcagtacg taaagccttt gaagatctgg aactggttat cgttcaggac
    21781 atctttatga ccaaaaccgc gtcggcggcg gatgtcattt tgccgtcaac gtcgtggggc
    21841 gagcatgaag gcgtgtttac tgcggctgac cgtggcttcc agcgtttctt caaagcagtt
    21901 gaaccgaaat gggatctgaa aacggactgg caaatcatca gtgaaatcgc cacccgtatg
    21961 ggttatccga tgcactacaa caacacccag gagatctggg atgagttgcg tcatctgtgc
    22021 ccggatttct acggtgcgac ttacgagaaa atgggcgaac tgggcttcat tcagtggcct
    22081 tgccgcgata cttcagatgc cgatcagggg acttcttatc tgtttaaaga gaagtttgat
    22141 accccgaacg gtctggcgca gttcttcacc tgcgactggg tagcgccaat cgacaaactc
    22201 accgacgagt acccgatggt actgtcaacg gtgcgtgaag ttggtcacta ctcttgccgt
    22261 tcgatgaccg gtaactgtgc ggcactggcg gcgctggctg atgaacctgg ctacgcacaa
    22321 atcaataccg aagacgccaa acgtctgggt attgaagatg aggcattggt ttgggtgcac
    22381 tcgcgtaaag gcaaaattat cacccgtgcg caggtcagcg atcgtccgaa caaaggggcg
    22441 atttacatga cctaccagtg gtggattggt gcctgtaacg agctggttac cgaaaactta
    22501 agcccgatta cgaaaacgcc ggaatacaaa tactgcgccg tgcgcgtcga gccgatcgcc
    22561 gatcagcgcg ccgccgagca gtacgtgatt gacgagtaca acaagttgaa aactcgcctg
    22621 cgcgaagcgg cactggcgta atacagtcct ttctacagcc tcctttcgga ggctgttttt
    22681 ttatccattc gaactcttta tactggttac tccctaccca atcgtattat caaaatgaaa
    22741 aaaattatcg cattgatgtt gtttttgaca ttctttgccc acgccaacga ctccgagcct
    22801 ggcagccagt atttaaaggc agcagaggcc ggggaccgac gcgcacaata ttttcttgcc
    22861 gacagctggt ttagctccgg cgatttgagc aaagccgaat attgggcaca gaaagccgcc
    22921 gacagcggtg atgctgatgc ctgcgcgctg ctggcgcaga tcaaaatcac caatccggtc
    22981 agtctggact atccacaagc aaaagttctt gcagagaaag cggcgcaagc gggcagtaaa
    23041 gaaggtgaag taacgctggc gcatattctg gtaaatactc aagcgggtaa accggattat
    23101 ccaaaggcta tttcgctgtt agaaaacgcc tcggaagatc tggagaacga ctctgccgtc
    23161 gatgcccaaa tgctgcttgg tttgatttac gccaacggcg tgggcattaa ggccgacgat
    23221 gacaaggcaa cctggtattt caaacgcagc tctgcaattt cccgaaccgg ttattccgag
    23281 tactgggcgg gaatgatgtt cttaaacggt gaagaaggtt ttatcgagaa gaacaagcaa
    23341 aaggcgctgc actggttgaa cctgagctgt atggaagggt ttgataccgg gtgtgaagag
    23401 tttgaaaaat taaccaacgg ttaaggtagg cctgataaga cgcggcaagc gtcgcatcag
    23461 gcattcacac attgccgggt ggatatcccc ggcaatcttc aattattgat ccgcagtttt
    23521 atcaaatttg cccagcactt cacgctcata agccagcgcc tttttgcggt caaatttgtg
    23581 ttcccacttg gcaatcacca gcaccgccag cgcattaccc accacgttca gcgcagtacg
    23641 cgccatgtcg aggatacggt caacaccagc aataaacgcc agaccttcca gcggaatacc
    23701 tacgctaccc agcgttgcca gcagcaccac aaacgacacg cccggcacgc cagcaatccc
    23761 tttcgaggtc accatcagcg tcagcaccag aatgatttcc tgccagatgg acaggtcaat
    23821 gccatacagc tgagcgatga aaatagcggc aatactttga tacagcgtcg aaccatcaag
    23881 gttaaaagag taaccggtcg gcaccacgaa gctggtgatc gacgccggtg ctccgtaggc
    23941 ttccatcttc tcaataattc gcggcagtac gctttcagag ctggcagtgg agtacgccag
    24001 aatcagctca tctttcagaa tacgaatcag gatccagacg cttaacccgc acaggcgcgc
    24061 cacaattccc agcactacca gcgcgaagaa cagaatggcg aaatgcacca gcagcaccag
    24121 tttcgccagt ggccacagag aagagaaacc aaagttagcc accgtcaccg caatcagcgc
    24181 aaacacaccc accggtgcat aacgcatcac catgtgagtc actttaaaca tggtttcaga
    24241 gatggagcgg aacacggtca ccagcggttc acgatgcgtc gcgggcaggg aagaaagccc
    24301 cagaccaaac agcaccgaga aaaagatgat cggcagcatt tcgcctttcg ccatcgacgc
    24361 cacaatgttc gtcggcacca gcgacaaaat cgtgcccata atgccgtggg aactgctttg
    24421 taccgcttcc gtagtgctct gatatttcga gatatcgacg gtcgccagct gcgacatatc
    24481 caccccggca ccgggctgga agacgttcgc cagagtgatc cccaaaatga tggcgacggt
    24541 ggtgatcacc tcgaagtaaa taatggtttt cgcgccaata cgcccgagct gtttggcatc
    24601 accaacaccc gcgataccca ccaccagcgt ggagatcaca atcggcacaa caatcatttt
    24661 gatcagatgg atgaagatat cacccgccgg agagagcaaa ttgacgacca gccagtcgcg
    24721 gctgtcgcta tggtagtgca ggtagcttcc caggagaatg cccagcacca tagcaaacag
    24781 aatctgccag gccaggctga attttatatt tttcatcaga ataacttcct caatggagcg
    24841 ccgttccccg ttcagaaacg cgagaacgtg acatactgcc agggcatgaa ttgtgttgcg
    24901 ttggtttgta gattttttta acgcttatga tcagtatcat ttgcgcggta tgttgcgcaa
    24961 ccctttaaca acagagcttt tcgctgcatt aatgcataat aacgcgagtt tatggcagga
    25021 ttaatgaata accatttgtt atggaaagat ttttatttgt ttaatctgca ataatactga
    25081 ttagtgatta tttttggcat atgttcattc gattattttt tatgcagctc aatgacataa
    25141 tcttcatcac cagatgatta atccgatctc cgctgcaaca gttttgccat attaatcgac
    25201 tcgaccagct gtgtccggtt gatccggggt gagttgagat ccatcacttt ttgccatagt
    25261 tcgatggctt gcgcgtaatt cgcctgcata aacgcatcgg aagccagcag catcagagcg
    25321 gtgatttcgt tagagtccag cgcgagggct ttgtcgatca ttgcgcaagt ctgggcggtc
    25381 atatgctggc tggcctggta ataaagcacc gtcgccagcg ccgcatacag ttcagcgttc
    25441 tcaccacgta gttgcaacgc ctgacgatac gccagcagcg aattgctgta atcgttttgc
    25501 cacagatagt actcgcccag taacgcccat tgttcgctgt tttgtggatt agcgcggatt
    25561 ttatcctgca atgcctgaag ctgcgcttcc ggggtttgct ggctggcaaa ctgatgtagc
    25621 ggatcgcgct gacgctgata ctccgcacgt accgcctgcc attttgggct taacagataa
    25681 ctgccgacac agaggaatac catcaatatc gtgagtgttg tcagcatttt caccggtatt
    25741 tgaggctgtt tcatcgctta gccctcactc gccagaggat cagtgccatc agcaataaca
    25801 acaccactgg cagcgcccac aacaccagcg tctgacccgt taacggtggg ttatagcgaa
    25861 caaaatctcc gtagcgttcg gtcatccagc caatgatttc gacttcgttc ttcccctccg
    25921 acaccatgct ataaacctga tggcgcatac tgaccgccac cggcgcattg gattcgagta
    25981 agttttgatt ctggcactgc ggacaacgta actggctggc aatatttaac gcctgttgct
    26041 gttgttgcgg attggcgaat tgccaggtgt ctacgacctg agcgcgcgca aaacaggtaa
    26101 ataacagcag tagcgtgaga agccctttat tcatcgcgct tcttcccccg ccatccgctt
    26161 aacaatgcgc ccgcaatcat caacaatcct ccaccccaga tccagcgcac accgctttgt
    26221 acatacaaac gaaaagcgta acgatccgct ccggtttttt cacccatgac cgcataccag
    26281 tcatggatgc cgttccagcg aattgacggt tccatcattt gctgacggcg tgcttcataa
    26341 aaacgccgct ccggcgttaa ttcaccaatg cgttgctgat ggtcaaacag cgccactatc
    26401 gctttttcgc tggtgtaatt gcctttggct tgcagatcga ggcgctcaaa acggaaggtg
    26461 tatcctgcca gcgtcacctg ctgacccggc tgtaaattca ggctgatctc ctgacggctg
    26521 acgctggaaa ccacgatccc tgcggcaaat aacagcacgc ccgcatgagc gaccagcgcc
    26581 ggaagctgcg cgcgtttgcc agagacaaac gtcgccagca caatcaccac cagcatcaac
    26641 agaccaaacg gtaacgtcgc gcggttaaaa tacggcgcgc caacggagag gcgtccccag
    26701 cctagcagac cgtaaatcat cggataaagc gttcccacca gcacgatcag tagcactgcg
    26761 caaaacagca acagcgtagc gaggattaac atttcccgcg ataaccccga aaaacgcacc
    26821 gccgggccac cgtcccgcgc tcgccagcca tacagagcca aagacgcaag gctaatcagt
    26881 gcaaacaggc tgaacaacgg cacggcgcgg acgttatcca gtgcgaacgc atgaaccgaa
    26941 accagaatgc cagaacggac aattaaggtg cccagcagcg acagcatcag agtaactatc
    27001 gccagcaaca gcgaccagtg gcaaaaaatc ccccgctggc gtgtcaggga taaactgtgc
    27061 agcagcgcag tggcagaaag ccagggtaat aaagaggcat tttccaccgg atcccagaac
    27121 caccagccgc cccagccgag ttcgcagtag gcccaccagg aaccgaggat gatccccgcc
    27181 gttaatgcac tccagccagg taacgcccag cgccagcaaa ttcgggcgct ggccgcatca
    27241 aactcgccgc gcagtagact cgccagcgcc acgctcgccg ctaccatcaa accgccatag
    27301 ccaaggtaaa gcagcggtgg atgaaagata agaccgggat gttgcagcat cggattgaga
    27361 tcgcggcctt cgattgctgg tggaaatatc cgcacgaagg gatcggacca cagcaccaca
    27421 aacagtagca gtgcggcgag catgagagat aaaacggcta acgtcagcgg aaatagcgga
    27481 tcggtttgct gccgataatg ccaggcaaac agcgcgctcc aggcggaaag cagcagcacc
    27541 caaagcagca gcgaaccttc atggccgccc cacaccgctg ccagcttgaa ttcccacgac
    27601 agcaggctat agctatgttg cgcgacataa atgaccgaga aatcgctgat gagaaaacaa
    27661 tacgtcagta cgccaaaggc gagcagcagg agcgcgaact gcgccagaat gccgatgcaa
    27721 gtgagtcgca tcatggcagg ccagcgcaac cgcactcccg caaaggccgt caacggggtc
    27781 aacacgttga ccccgagact taacaacaac gccagaaaac cgacttcagg aaggaatacg
    27841 tccagaaacc acctcttttc tggcttacgc caccgtcaac tgtccggcgt agagaatgaa
    27901 aaagcgcagc atcaacacgc cgaccagact ggccccacac gccgccagca cggcaggaat
    27961 gccggaactg cgattgaccc acggtttgag caacattggc acaatcagcc ccagcccggc
    28021 gacaccaagc cagaaccacc acgtccagaa accgccacct aatgccgcca ccagcgcacg
    28081 aactttaccg tcatcgccca gcgccagacc gacaaaaaac gccaccagca ggaagatttc
    28141 accccatacc acggggattt ccatccggtg aacaaagtgc gcttccgtgg aatgcgggtt
    28201 actgcgttgg cgtatcgcca tggcgatcag cgccaccgcc gcaccggacg agatgccgga
    28261 gaagaggaac agcaccggca ggatcgggtt attgaggaac ggatacgatt tcagcgccga
    28321 cagcagaaag ccggtataag cccccagcag caccgccaac accagcatca gcgtttccag
    28381 tccgcgatga acgggcgtca gcaggctcag aaccttttgc acgatcccca gcttcggcaa
    28441 ccagcgttgt tgcagggcaa gcaaatcatg ttcaaagatt ttcgccagcc acagcaccag
    28501 caccaccatg tagagctgaa acagcatcac ccccatcgac atcaccgagg taaaactgta
    28561 gtggaacatc agcttccaga aggtccacgg tcttgtcagg tggaagacga ggatcaacag
    28621 accgaggatc accgcgcccg gcccgacaat cagcgtggtg cgcagcaacg tactgtctgc
    28681 accgcccgcc tgcgggtaga agcgacgtaa cagcacggcc agcgtcacca gaccggcaga
    28741 aataccaatc aaaaacaggt agatggcaat cggccagtcc cacaccagcg attcaaaatg
    28801 aaatgcggaa gtctgcgtca ttggctcacc tccccgtatt taaacggtac gcggtacagc
    28861 ttcggtttgg ttcccagcgc cagcttgtag cggtaagtag gcttctggcg cagcagttgc
    28921 gaaatctcac tgttaggatc gtccagattg ccaaacgtca gcgccttcgt cgggcaagcc
    28981 tcaacacacg cgggcagctt accggcctgc aaattggttt tacggcagaa atcgcatttg
    29041 tccgccgtct tcgtgaccgg atggataaag cgcacgcggt acggacaggc ggcgatgcag
    29101 tactgacaac cgacgcagag atcaggatta acatcgacaa tcccgctagc ggcatcacga
    29161 aaagacgcac cggtcgggca gacgtcaacg cacggcgcat gatcgcagtg ctggcaagac
    29221 ttacggaaga agcgatattt cacatcagga aattcgccct gcggctcgct acgaataatc
    29281 gtcaggcgcg agacgccttc cggcaccttg ttcacttccc gacaagcatc catacaggcg
    29341 gtacagccga tgcataacga ctcgtcatgc accatgccgt aacgcacccc attgatattc
    29401 aacgttttcg ccaccacgcg ccctgcggtg ccgctgacgg ctgccagcac gccgacgccg
    29461 gtgagaaact ggcgacgaga ccaggtcatg gctgctcctt aagcaacgga acagacgccg
    29521 ggttaaagtt cggattggtg cgctgatcgc tgtggcaatc gacgcaaatc ttaatccgtc
    29581 ctttgtcact taacgtctgc atcgtatctt gctgtggatg cagggaatgg cagctggcgc
    29641 acgccacttt ggttacgtgg acatcgtgcg gccagaacgc tttttgcaac tgttccggca
    29701 gatgacagga catacagacg ctgttctgct ctccaacctt gtacatcggc tcgttaaagc
    29761 gcatcacatc tttcacccct tcgcggtgtt gcggtgatgg ctggccgtga cagttggtgc
    29821 aggtgaccgg cagtttgtta ttcgggttga taacggaagc atgtttgcca tgcatacctt
    29881 cggtatctgg tttatgacag tccagacagg cggcatccgg gttacgctgc tgggtaactt
    29941 cgtagcgatc atcagatgcc tgcgccgcag gggtagcggt aattccgttc aggctccaca
    30001 acaggcccga cgccagcacc ccggcagtta ataacgaacg taatacgctc atattcactc
    30061 cattgtgttt tccgcccctt gcgaggcgga acggggttat tggcttaaca gaccgttttt
    30121 acgtgcctgt tcttcccact gcgggatcac cgttttgatg aagtcctgct tctcggcctt
    30181 gatttgttcc atgttcaggc caatggcctg ctgggctttc tctttggttg agatatccgg
    30241 gatctggatt tcatgggtga tgcctttggt cgccagcagg cgcgccagtt tggtgcgtgc
    30301 atccgccgct ttatccatcg ccgtaccgag catccgtaaa ccttcttccg gtgcgtgcat
    30361 atgaatgccg tgggaagcga tcgccagatc ccagcgccac tgggcatgac ggatatcgtc
    30421 ctgaattggc ttcatttcag cttccgtcgc gcctgcatcc agcgctgctt tcgcttcgaa
    30481 gtgagcgtga accagttgat cttcaacctt gattttcagg tcgttaatcg actgcttacg
    30541 ttccgcgacc actttttgca aggcagcttt gtcctgggta tggcagttcg cacaagtctg
    30601 ggcgaagtta tcaaacggat taccaatttt atggtcggta tagagtttgc cttcggcgtt
    30661 ctgcactttt ggcatatggc agtcgataca ggtcacgttg tttttaccgt gaatgcccgc
    30721 tgtccaggtt tcatattccg ggtgctgcgc tttcagcatt ggcgttttcg acagggagtt
    30781 agtccagtca gagaaggcaa ttttgtcgta atactgctcc atattttcga ctttcatgcc
    30841 gtcatcccac gggaatttaa ccgctttgtt tttgccgtcg aagtaatact ccacatggca
    30901 ctgaccgcaa accatcgatt gctggtcgaa acgtccggct ttctcaaaag gtttaccaat
    30961 ggcttccatc gcgcgagccg catacggacg ggaaagggtt aactccggtt tgcctttggc
    31021 gaactctggt gaggcggtgt tatggcagtc ggcacaacct aagttgttga cgatttccgg
    31081 gccgccgcgc gcccacttac cgtggaagta gccatcttcg ccgtctttct ggatcagacg
    31141 cgccacatcc gggcttttac aactccagca cgccatcggt agcggaccat cttcagcgtt
    31201 tttcggcgca ccggtacgca gggtttcacg cacatcggtc acagcaaagg catgtccacg
    31261 cggcttgttg taatcgcgcg agaagggata ccccgcccac aggatcacca gccgtggatc
    31321 ttccgccagg gcgtcaacac gctctgactg ttccgaggtg gctttccagg agagatattg
    31381 atcgggatgc tgcggggcaa aggtttcatt cttcgcttcc acagttacag gttttgcggg
    31441 agctgccgtt tgttcagcgt gaacagaagt gaaaaagaaa aaaggaatca ataagctgaa
    31501 gatacggcgt gcgtttattt ttatccttgt cataggggct tcatccgaat tgcgccattg
    31561 ttacaatggc ggtttttatt gtttttcacg acagtaaccg cacctacact gtcatgacat
    31621 tgctcgcccc tatgtgtaac aaataaccac actgtgaatg ttgtctttaa tcaattgtaa
    31681 gtgcatgtaa aataccactt tagagttagt tagtatcttc ctctttttca acagcaatgc
    31741 ataactgcat gttcctcaaa gaattaatca acttttgttg ctgaccttca aaaattaccc
    31801 tgccgtttat ttgcacaatt ctacttttgc gtgatctgtc gcccaaatac taaacaaaac
    31861 tgccaatacc cctacattta acgcttatgc cacatattat taacacccta caaggagaac
    31921 aaaagcatga gccaaattca caaacacacc attcctgcca acatcgcaga ccgttgcctg
    31981 ataaaccctc agcagtacga ggcgatgtat caacaatcta ttaacgtacc tgataccttc
    32041 tggggcgaac agggaaaaat tcttgactgg atcaaacctt accagaaggt gaaaaacacc
    32101 tcctttgccc ccggtaatgt gtccattaaa tggtacgagg acggcacgct gaatctggcg
    32161 gcaaactgcc ttgaccgcca tctgcaagaa aacggcgatc gtaccgccat catctgggaa
    32221 ggcgacgacg ccagccagag caaacatatc agctataaag agctgcaccg cgacgtctgc
    32281 cgcttcgcca ataccctgct cgagctgggc attaaaaaag gtgatgtggt ggcgatttat
    32341 atgccgatgg tgccggaagc cgcggttgcg atgctggcct gcgcccgcat tggcgcggtg
    32401 cattcggtga ttttcggcgg cttctcgccg gaagccgttg ccgggcgcat tattgattcc
    32461 aactcacgac tggtgatcac ttccgacgaa ggtgtgcgtg ccgggcgcag tattccgctg
    32521 aagaaaaacg ttgatgacgc gctgaaaaac ccgaacgtca ccagcgtaga gcatgtggtg
    32581 gtactgaagc gtactggcgg gaaaattgac tggcaggaag ggcgcgacct gtggtggcac
    32641 gacctggttg agcaagcgag cgatcagcac caggcggaaa agatgaacgc cgaagatccg
    32701 ctgtttattc tctacacctc cggttctacc ggtaagccaa aaggtgtgct gcatactacc
    32761 ggcggttatc tggtgtacgc ggcgctgacc tttaaatatg tctttgatta tcatccgggt
    32821 gatatctact ggtgcaccgc cgatgtgggc tgggtgaccg gacacagtta cttgctgtac
    32881 ggcccgctgg cctgcggtgc gaccacgctg atgtttgaag gcgtacccaa ctggccgacg
    32941 cctgcccgta tggcgcaggt ggtggacaag catcaggtca atattctcta taccgcaccc
    33001 acggcgatcc gcgcgctgat ggcggaaggc gataaagcga tcgagggtac cgaccgttcg
    33061 tcgctgcgca ttctcggttc cgtgggcgag ccaattaacc cggaagcgtg ggagtggtac
    33121 tggaaaaaaa tcggcaacga gaaatgtccg gtggtcgata cctggtggca gaccgaaact
    33181 ggcggtttca tgatcacgcc gctgcctggc gctaccgagc tgaaagccgg ttcggcaaca
    33241 cgtccgttct tcggcgtgca accggcgctg gtcgataacg aaggtaaccc gctggaaggc
    33301 gctaccgaag gcagcctggt gatcaccgac tcctggccgg gtcaggcgcg tacgctgttt
    33361 ggcgatcacg aacgttttga acagacctac ttctccacct tcaaaaatat gtatttcagc
    33421 ggcgacggcg cgcgtcgcga tgaagatggc tattactgga tcaccgggcg tgtggacgac
    33481 gtgctgaacg tctccggtca ccgtctgggg acggcagaga ttgagtcggc gctggtggcg
    33541 catccgaaga ttgccgaagc cgccgtagta ggtattccgc acaatattaa aggtcaggcg
    33601 atctacgcct acgtcacgct taatcacggg gaggaaccgt caccagaact gtacgcagaa
    33661 gtccgcaact gggtgcgtaa agagattggc ccgctggcga cgccagacgt gctgcactgg
    33721 accgactccc tgcctaaaac ccgctccggc aaaattatgc gccgtattct gcgcaaaatt
    33781 gcggcgggcg ataccagcaa cctgggcgat acctcgacgc ttgccgatcc tggcgtagtc
    33841 gagaagctgc ttgaagagaa gcaggctatc gcgatgccat cgtaacccac aattgccgga
    33901 tgcggcgtaa acgccttatc cggcctacat tcggcaaggg ttacccgagc gttaaccttc
    33961 tcccataagg gagcgggaat taaaacaatc cctacattac ctctggagaa tctgtgatga
    34021 atggcactat ttatcagcgg atagaagaca atgcgcattt cagggagtta gtcgaaaaac
    34081 ggcaacggtt tgccaccatc ctgtcgatta ttatgctggc agtttatatc ggctttattt
    34141 tactgatcgc cttcgcgccc ggctggctgg cacgccgctg aatccgaaca ccagcgtcac
    34201 acgcggtatt ccaattggtg ttggagtgat tgtgatctcc tttgttctca ccggtatcta
    34261 catctggcgg gcgaacggcg aattcgaccg tcttaataat gaagtcctgc atgaggtaca
    34321 agcatcatga aaagagttct gacggcgctt gccgccacac tccctttcgc agctaacgcc
    34381 gcggatgcta ttagcggggc cgtagagcgc cagccaacga actggcaggc gattattatg
    34441 ttcctgattt tcgtcgtgtt tacgctcggc attacctact gggcatcaaa actcgtacgt
    34501 tctcgtagcg actactacac cgcaggcggc aatatcactg gcttccagaa cgggctggcg
    34561 attgccgggg actatatgtc cgccgcctca ttcttgggga tctccgcgct ggtgtttacc
    34621 tccggctatg acggcttaat ttactcgctg ggcttcctgg tgggctggcc gatcattttg
    34681 ttcctgattg ccgaacgtct gcgtaacctg gggcgctaca cctttgccga tgtggcctct
    34741 taccgtctga aacaagggcc gattcgtatt ctttcggcct gtggttctct ggtggtggtg
    34801 gcgctttacc ttatcgccca gatggtgggc gcaggtaaac tgatcgagct gctgtttggc
    34861 cttaactatc acattgcggt ggtgctggtc ggcgtgctga tgatgatgta cgtcctgttc
    34921 ggcggcatgc tggcgaccac ttgggtacag attatcaaag ccgtgctgtt gctgttcggt
    34981 gccagcttta tggcctttat ggtgatgaaa cacgtcggct ttagcttcaa caatctgttc
    35041 agtgaagcga tggcggtaca cccgaaaggt gtcgacatca tgaagccggg cgggctggtg
    35101 aaagatccga tctccgcact ctctctgggt ctgggactga tgtttggtac ggcgggcttg
    35161 ccgcacattc tgatgcgctt ctttacagtc agcgatgccc gcgaagcacg taagagcgtg
    35221 ttctacgcca ccgggtttat gggctacttc tatattctga cctttattat cggcttcggc
    35281 gcgatcatgc tggttggtgc gaatccggaa tataaagacg cggcgggcca tctgattggt
    35341 ggtaacaaca tggcggccgt tcacctggcg aatgcagtgg gcggcaacct gttcctcggt
    35401 tttatttcag cggttgcttt cgccactatc ctcgcggtgg ttgcgggtct gacgctggcg
    35461 ggcgcatccg cggtttcgca tgacttgtac gctaacgtct tcaaaaaagg cgcgaccgaa
    35521 cgtgaagagc tgcgggtatc aaaaatcacc gtactgatcc tcggcgtgat tgcgattatc
    35581 ctcggcgtgc tgtttgagaa tcagaacatc gcctttatgg tggggctggc gtttgccatc
    35641 gcggcgagct gtaacttccc gatcattctg ctttctatgt actggtcgaa actgaccacg
    35701 cgtggcgcga tgatgggtgg ctggctgggg ctgattaccg cagtagtact gatgatcctc
    35761 ggcccgacga tttgggtaca gatccttggt cacgaaaaag ccatcttccc gtatgaatac
    35821 ccggcgctgt tctctatcac cgtggcattc ctcggcatct ggttcttctc ggcaaccgat
    35881 aactcagcgg aaggggcgcg cgagcgtgaa ctgttccgcg cgcagtttat ccgctcccag
    35941 accggctttg gcgttgagca aggccgcgca cattaatctc tccccttccc cggtcgcctg
    36001 accggggaat actcttcctc tccagcatgc atcacctttt cccaaaatat taaacaaata
    36061 aactcattaa aaaatgagcg atttttgaca gtcgtagaaa atgata
//
LOCUS       DADMKF010000027        35163 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.027, whole genome shotgun sequence.
ACCESSION   DADMKF010000027 DADMKF010000000
VERSION     DADMKF010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35163
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.027"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(<1..384)
                     /locus_tag="JNC24_001201"
     CDS             complement(<1..384)
                     /locus_tag="JNC24_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005136105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9158009.1"
                     /translation="MLREVSISNDTISVKFYRNEKIECACNFLMDKDAQGYIDLSDFD
                     LINCHFKGDVISKVSFLSSNLQHVTFECKEIENCNFTKATVDNVIFKCRRLHNVIFLK
                     TSGECVDFSQNILDTVDFSQSQLGHS"
     gene            complement(387..581)
                     /locus_tag="JNC24_001202"
     CDS             complement(387..581)
                     /locus_tag="JNC24_001202"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9158010.1"
                     /translation="MIGHITNSGGNNSLMKHDVVQGNNIVDLDLLRNFNGVTGLNRDN
                     FIYISDIFLNIKQRNEKIMQ"
     gene            complement(810..1730)
                     /gene="malM"
                     /locus_tag="JNC24_001203"
     CDS             complement(810..1730)
                     /gene="malM"
                     /locus_tag="JNC24_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HAY9158011.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQEPGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTSDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPVPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            complement(1882..3222)
                     /gene="lamB"
                     /locus_tag="JNC24_001204"
     CDS             complement(1882..3222)
                     /gene="lamB"
                     /locus_tag="JNC24_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HAY9158012.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            complement(3294..4409)
                     /gene="malK"
                     /locus_tag="JNC24_001205"
     CDS             complement(3294..4409)
                     /gene="malK"
                     /locus_tag="JNC24_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HAY9158013.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIHIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            4774..5964
                     /gene="malE"
                     /locus_tag="JNC24_001206"
     CDS             4774..5964
                     /gene="malE"
                     /locus_tag="JNC24_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HAY9158014.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVAVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            6118..7662
                     /gene="malF"
                     /locus_tag="JNC24_001207"
     CDS             6118..7662
                     /gene="malF"
                     /locus_tag="JNC24_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HAY9158015.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ECAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGEIGKNYLSDAFKFGGEQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGADPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLLFLLVGALAIVNLKA
                     TRMKFD"
     gene            7677..8567
                     /gene="malG"
                     /locus_tag="JNC24_001208"
     CDS             7677..8567
                     /gene="malG"
                     /locus_tag="JNC24_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HAY9158016.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     AIGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            8939..10414
                     /gene="xylE"
                     /locus_tag="JNC24_001209"
     CDS             8939..10414
                     /gene="xylE"
                     /locus_tag="JNC24_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HAY9158017.1"
                     /translation="MNTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLNTVFVA
                     PQNLSESAANSLLGFCVASALIGCIIGGALGGYCSNRFGRRDSLKIAAVLFFISGVGS
                     AWPELGFTSINPDNTVPIYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIR
                     GKLVSFNQFAIIFGQLLVYCVNYFIARSGDASWLNTDGWRYMFASECIPALLFLMLLY
                     TVPESPRWLMSRGKQEQAESILRKIMGNTLATQAVQEIKHSLDHGRKTGGRLLMFGVG
                     VIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIM
                     TVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQAPGIVALLSMLFYVAAFAMSWGPVCW
                     VPLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHNGFSYWIYGC
                     MGVLAALFMWKFVPETKGKTLEELEALWEPETKKTQQTATL"
     gene            complement(10458..10868)
                     /gene="psiE"
                     /locus_tag="JNC24_001210"
     CDS             complement(10458..10868)
                     /gene="psiE"
                     /locus_tag="JNC24_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HAY9158018.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(10994..11137)
                     /locus_tag="JNC24_001211"
     CDS             complement(10994..11137)
                     /locus_tag="JNC24_001211"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9158019.1"
                     /translation="MFQRNIVDFSRIAGGWESPEWVIFELPAWVRFVLPDAPPGAAYP
                     AYG"
     gene            11138..11416
                     /locus_tag="JNC24_001212"
     CDS             11138..11416
                     /locus_tag="JNC24_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9158020.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            complement(11463..13559)
                     /locus_tag="JNC24_001213"
     CDS             complement(11463..13559)
                     /locus_tag="JNC24_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HAY9158021.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYIDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETTLAQKRVEPVVPQSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTIGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDAYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(13559..14296)
                     /locus_tag="JNC24_001214"
     CDS             complement(13559..14296)
                     /locus_tag="JNC24_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HAY9158022.1"
                     /translation="MIKQTIVALLLSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAEQGADSSTDDAAAINALRQ
                     QIQALEVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISHPGNQP
                     FTPGRDVASYLSDQSLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            complement(14293..14931)
                     /locus_tag="JNC24_001215"
     CDS             complement(14293..14931)
                     /locus_tag="JNC24_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HAY9158023.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     LVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            complement(15045..15287)
                     /locus_tag="JNC24_001216"
     CDS             complement(15045..15287)
                     /locus_tag="JNC24_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9158024.1"
                     /translation="MKKVLYGIFAISALAATSVWAAPVQVGEAAGAAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            complement(15749..17398)
                     /gene="pgi"
                     /locus_tag="JNC24_001217"
     CDS             complement(15749..17398)
                     /gene="pgi"
                     /locus_tag="JNC24_001217"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HAY9158025.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     gene            17923..19272
                     /gene="lysC"
                     /locus_tag="JNC24_001218"
     CDS             17923..19272
                     /gene="lysC"
                     /locus_tag="JNC24_001218"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HAY9158026.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHSNLFE"
     gene            19524..19796
                     /locus_tag="JNC24_001219"
     CDS             19524..19796
                     /locus_tag="JNC24_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HAY9158027.1"
                     /translation="MALPRITQKEMTEREQRELKTLLDRARIAHGRVLTNSETNSIKK
                     EYIDKLMVEREAEAKKARQLKKKQAYKPDPEASFSWSANTSTRGRR"
     gene            complement(19929..20801)
                     /gene="rluF"
                     /locus_tag="JNC24_001220"
     CDS             complement(19929..20801)
                     /gene="rluF"
                     /locus_tag="JNC24_001220"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HAY9158028.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDIVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            21149..21986
                     /locus_tag="JNC24_001221"
                     /pseudo
     CDS             21149..21986
                     /locus_tag="JNC24_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313031.3"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
     gene            22056..22862
                     /locus_tag="JNC24_001222"
     CDS             22056..22862
                     /locus_tag="JNC24_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAY9158029.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEVHKGYQFWPTDISSAKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            22872..23279
                     /locus_tag="JNC24_001223"
     CDS             22872..23279
                     /locus_tag="JNC24_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbose transporter subunit IIA"
                     /protein_id="HAY9158030.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGDANVEAVEFVPGENAGDIV
                     AKLEKLVSIHNQDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNVDELCEHLTQIAKQTCVVWKQLATAEEDF"
     gene            23279..23773
                     /locus_tag="JNC24_001224"
                     /pseudo
     CDS             23279..23773
                     /locus_tag="JNC24_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
     gene            23839..24636
                     /locus_tag="JNC24_001225"
     CDS             23839..24636
                     /locus_tag="JNC24_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HAY9158031.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALGWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLNAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALVYIQLNPQWRKAEPQLQATASTALDQLDD"
     gene            24647..25471
                     /locus_tag="JNC24_001226"
     CDS             24647..25471
                     /locus_tag="JNC24_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAY9158032.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRRHLVFFNTTPAVCGPVIGVTAAMEEARANGAEIDDGTINGIKVG
                     LMGPLAGVGDPLVWGTLRPITAALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQL
                     GFRKGVNIVSDMGGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTHAADGST
                     VIMTVQNILDQLCPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            25523..26751
                     /locus_tag="JNC24_001227"
                     /pseudo
     CDS             25523..26751
                     /locus_tag="JNC24_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312968.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
     gene            27134..27823
                     /gene="pepE"
                     /locus_tag="JNC24_001228"
     CDS             27134..27823
                     /gene="pepE"
                     /locus_tag="JNC24_001228"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HAY9158033.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TCDDYTAKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(27914..29545)
                     /locus_tag="JNC24_001229"
     CDS             complement(27914..29545)
                     /locus_tag="JNC24_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HAY9158034.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(29765..33448)
                     /gene="metH"
                     /locus_tag="JNC24_001230"
     CDS             complement(29765..33448)
                     /gene="metH"
                     /locus_tag="JNC24_001230"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000096005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HAY9158035.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDGLV
                     VAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFETLGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAEAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGEDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWEVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNEPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKTDDTANAQQAEWRSWEVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRTAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTRKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            33648..34472
                     /gene="iclR"
                     /locus_tag="JNC24_001231"
     CDS             33648..34472
                     /gene="iclR"
                     /locus_tag="JNC24_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HAY9158036.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
     gene            34789..35163
                     /locus_tag="JNC24_001232"
                     /pseudo
     CDS             34789..35163
                     /locus_tag="JNC24_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         8387 a         9118 c         8981 g         8677 t
ORIGIN      
        1 actatgacca agttgactct gcgagaagtc aactgtatca agaatatttt ggctaaaatc
       61 aacacattcg ccactcgttt tgagaaaaat cacattgtgt aaacgtcgac atttaaatat
      121 gacattatca actgttgcct tagtaaaatt gcaattctca atttctttac attcgaaagt
      181 tacatgttgt agatttgatg ataaaaaaga caccttagaa ataacgtcac ctttaaaatg
      241 acaatttatt aaatcaaaat cagacaggtc gatatacccc tgcgcatctt tatccatcag
      301 aaaattacaa gcgcattcta ttttttcatt tctgtagaat tttacactta tagtatcatt
      361 actaattgag acttcacgta acatatttat tgcatgattt tttcgttccg ttgctttata
      421 tttaaaaata tatcactgat ataaataaag ttatctctgt ttaaacctgt caccccattg
      481 aaattacgta gtaaatcaag atctactata ttatttcctt gaaccacatc atgtttcatc
      541 aaagagttat tgccgccaga atttgtgata tgacctatca taaaacctct gtaatacaga
      601 aataaaataa cctggaaaag tagcctgagc gtccagacac taccggcgaa aggaaaagaa
      661 tctctgataa ggcattgaga taatggacat tcttagtgaa gaagaagtga agtactataa
      721 aaaatcgctc atatcccagg aaggaagcat aaaaaaaggt gcgccaggag acgcaccagt
      781 tgttgcaaaa tcagcactgt ggggcgtaat taccccttgc cttttacact gctgataaag
      841 gtggaacggg cagatgtcga tcccaggcgt tcagcttcat caagcagttt taacgcctta
      901 tcaacatcac ctttcgcgac agcgtttttg atcgcggtat taaaataact ttccgtgtcg
      961 ttgagcatcg gctcgctttt cttcaccggc accggagcag gtgcagccac agctggtgcc
     1021 gccgtgttgc ctaccgtaac cggagctgga gcggaagaac caaatagtgg tcctaccaac
     1081 acgctggagc tggagttcgt tttgactttc agtttcagta agccatcgct ggtatgacga
     1141 gcaaccggat cgggaatatc cgggatcgag ttaccgacgc ctttggcata ggctttagcc
     1201 gggtcgagca gttgggtcgt ctgctggaga tctttttccg tggtaaagac cagaacataa
     1261 agtttttgct gccccaacgc cggtgtcagg cgcataacgc cttccagccg atctgcactc
     1321 atcacgcctg gttcctggta ggtgaaataa ctgctgggga agaaggctga tggggtcatg
     1381 ttctgatcaa gaatcagcac gttcggcgca aaaacgctgg tttgtttgtt cacttcgctg
     1441 gtcagcgtca gggtcagttc gccaatgttt gccgggacgc tgtacgcagc aaccggacca
     1501 ctgatgccgg gaacgttcag ttgttggccg ccggtcgcca gttgggtggt ctgggtttta
     1561 gattgatcga ccggtgtcca ggtgagttgt tgcagcgcgg cagatggaat ggctggcgcg
     1621 tcgctggtgt tttgcggtac gtagttaaca tcggcaaggc taattccagg cgcgcttgcc
     1681 agtaaccctg ctgataaaca gagggcgatg agacttttat tcattttcat tgttttcacc
     1741 tcaaaatctg gagctcagcg gtagccaggc aatagcgcgc taaacccgat aatcagaggg
     1801 gctttcgccc ctttcagata atgacaacct gtttttatgc cggatgcggc gtaaacgcct
     1861 tatccggccc aggttttgct attaccacca gatttccatc tgggcaccga aggtccactc
     1921 gtcgctgtcg ccacgaccga agctgccgcc gttgaaatca gcaggaacgg ctttgccgta
     1981 gttcgggtta accttgctat cgccgttgta gtcgtaaccc catttctcat cccacttggc
     2041 gtaggttgcg aagacacgaa tagccgggcg tgaccagatg ctgtcgccag cctgccattg
     2101 ttgtgcgagg gtaattttgt actgattgtt cttgtcgccg gtgcgctggg attcgacgtt
     2161 gtcgtagccg atttccatca cggtgctcat gattggcgtc cacttgtaca tcgggcgaat
     2221 accgacggtc caccacttgg tgccgttgtc gttatcccag ttgatatcct ggtacatacc
     2281 cacgtacatc atgtcccagt tgtcgcccat ggagatcgca ccgtggtcga ggatacgcag
     2341 catgtgaccg ttgttgttga tattgtaggc aaatttttcg ttatcaaacg cgacgccaga
     2401 accctgcgac agacctttac cctgcgaggt catcgagtca gtagcgtact gaacaacaaa
     2461 cttgttaaag cccttcagga cactctgagt atgttcagca gtgaataacc agccgtcttt
     2521 cgatgcgcca tcaaccagac gatagttatc acgcaggttg gcacgaccgt agtcgacacc
     2581 cagttctaat gtgccgcccg ggttgatttc catctgcgct aaacgcacat cgaaaacgtc
     2641 gttcgcggtt tcgttggtat agtcataaat attgttgctg gcaaaagagg aagaaccacc
     2701 agcttcagag gagcgggttg ctgccagaga gagtttaccg aagccaacat cgatgttttc
     2761 cagaccggca ccaggaccag aaatatccca gtagtagaag tcgatcatat gaacgtcatg
     2821 acgttggtag aagcgcttac ctgcccagat ggtggaacct ggcagccatt cgatcaggtt
     2881 tttaccctgc acgtttgctt cacggaaggc cggatcggta gcttcccagt cattctgttg
     2941 cgcgacggaa taggccacgt tagtgtcgaa atagaagctc ttatcgccct ctttccacac
     3001 ttcctgaccc aattttaatt cagcataagt ttcacattcg ttgccaagac ggtatttact
     3061 ttgagcaccg gtagtctgga aacactgttg ttcaccgccg ctacctgtcc agccaatacc
     3121 ggaacgtgca tagccgtgga aatcaacagc cattgcctga gcagacatta cgcccgctgc
     3181 gacggcaacc gccagaggaa gtttgcgcag agtaatcatc attctatctc ctgagtcatt
     3241 gcttttcttt tttcacatca cctgtgacag gctttgtgtg ttttgtgggg tgcttaaacg
     3301 cccggctcct tatgcagtcg acgacatgca gtgccatcct cacggaacag atggcaacgc
     3361 tctggcggca ggccgatagc gaatgtggca ccttcttcta ccaacaccac gtcgttctgg
     3421 cggtacacca ggttttgacg aatggaaggg atctggatat ggatttgggt ttcgttgccg
     3481 agttgctcga cgacctgaac ttcaccctca aggatgacgt cagcgatatc actcggcagt
     3541 agatgttccg ggcgaatacc cagcgacata ttggctccaa cctggacatc acggctttca
     3601 actggcagcc agacttgctg acgatttggc atcggcagct ccacctgcac ttgatcgatt
     3661 gcggtggcgg tcacttttac cggcaggaag ttcatctttg gcgaaccgat aaatccggcg
     3721 acaaaacggt ctgccggata gtggtacagc tccagcggtt tcccaacctg cgccacgcga
     3781 ccggcgtcca gcaccacgat tttgtcggcc agcgtcatcg cttcgacctg atcgtgggtg
     3841 acgtaaatca ttgtgcggcc caggcgttta tgcagacggg agatttcgat acgcatttgc
     3901 acacgcagtg cagcatcgag gttggagagc ggttcatcga gcaaaaatac gcttggctcg
     3961 gccaccagcg tacggccaat cgccacacgc tgacgctgac caccggagag cgctttcggt
     4021 ttgcgatcca gcaaatgcgc cagttgcagc acttccgcca cctggttgac gcgttggtta
     4081 atcacctctt tttttgcgcc agccagtttc aggccaaatg acatgttttc tgctactgac
     4141 aggtggggat agagcgcgta agactgaaac accataccaa cgccgcgttc tgctggcgga
     4201 gtgtcattca tccgtttctc accgatgaac aggtcgccgc tggtgatcgt ctcaagcccg
     4261 gcaatcatgc gcagtaaagt cgatttaccg cagccagacg gtccgacaaa caccacgaat
     4321 tcaccttcat ggatatcgag attgatatct ttcgatacca cgacctcgcc ccaggctttc
     4381 gttacatttt gcagctgtac gctcgccatg cccttctccc tttgtaacaa cctgtcatcg
     4441 acagcaacat tcatgatggg ctgactatgc gtcatcagga gatggcttaa atcctccacc
     4501 ccctggcttt tttatggggg aggaggcggg aggatgagaa cacggcttct gtgaactaaa
     4561 ccgaggtcat gtaaggaatt tcgtgatgtt gcttgcaaaa atcgtggcga ttttatgtgc
     4621 gcatctccac attaccgcca attctgtaac agagatcaca caaagcaacg gtggggcgta
     4681 ggggcaagga ggatggaaag aggttgccgt ataaagaaac tagagtccgt ttaggtgttt
     4741 tcacgagcac ttcaccaaca aggaccatag attatgaaaa taaaaacagg tgcacgcatc
     4801 ctcgcattat ccgcattaac gacgatgatg ttttccgcct cggctctcgc caaaatcgaa
     4861 gaaggtaaac tggtaatctg gattaacggc gataaaggct ataacggtct cgctgaagtc
     4921 ggtaagaaat tcgagaaaga taccggaatt aaagtcaccg ttgagcatcc ggataaactg
     4981 gaagagaaat tccctcaggt tgcggcaact ggcgatggcc ctgacattat cttctgggcg
     5041 cacgaccgtt ttggtggcta cgctcaatct ggcctgttgg ctgaaatcac cccggacaaa
     5101 gcgttccagg acaagctgta tccgtttacc tgggatgccg tacgttacaa cggcaagctg
     5161 attgcttacc cgatcgctgt tgaagcgtta tcgctgattt ataacaaaga tctgctgccg
     5221 aacccaccaa aaacctggga agagatcccg gcgctggata aagaactgaa agcgaaaggt
     5281 aagagcgcgc tgatgttcaa cctgcaagaa ccgtacttca cctggccgct gattgctgct
     5341 gacgggggtt atgcgttcaa gtatgaaaac ggcaagtacg atattaaaga cgtggccgtg
     5401 gataacgctg gcgcgaaagc gggtctgacc ttcctggttg accttattaa aaacaaacac
     5461 atgaatgcag acaccgatta ctccatcgca gaagctgcct ttaataaagg cgaaacagcg
     5521 atgaccatca acggcccgtg ggcatggtcc aacatcgaca ccagcaaagt gaattatggt
     5581 gtaacggtac tgccgacctt caagggtcaa ccatctaaac cgttcgttgg cgtgctgagc
     5641 gccggtatta acgccgccag tccgaacaaa gagctggcga aagagttcct cgaaaactat
     5701 ctgctgactg acgaaggtct ggaagcggtc aataaagaca aaccgctggg tgcagtggcg
     5761 ctgaagtctt acgaggaaga gttggcgaaa gatccacgta ttgccgccac catggaaaac
     5821 gcccagaaag gtgaaatcat gccgaacatc ccgcagatgt ccgctttctg gtatgccgtg
     5881 cgtactgcgg tgatcaacgc cgccagcggt cgtcagactg tcgatgaagc cctgaaagac
     5941 gcgcagactc gtatcaccaa gtaatgctgt gaaatgccgg atgcggcgtg aacgccttgt
     6001 ccggcctaca aaactgaaac gcatgtaggc ctgataagac gcatcagtgt cgcatcaggc
     6061 agttgttgtc ggataaggcg tgaaagcctt atccgtcctg gaatgaggaa gaaccccatg
     6121 gatgtcatta aaaagaaaca ttggtggcaa agcgacgcgc tgaaatggtc agtgctaggt
     6181 ctgctcggcc tgctggtggg ttaccttgtt gttttaatgt acgcacaagg ggaatacctg
     6241 ttcgccatta ccacgctgat attgagttca gcggggctgt atattttcgc caatcgtaaa
     6301 gcctacgcct ggcgctatgt ttacccggga atggctggaa tgggattatt cgtcctcttc
     6361 cctctggtct gcaccatcgc cattgccttc accaactaca gcagcactaa ccagctgact
     6421 tttgaatgtg cgcaggaagt gttgttagat cgctcctggc aagcaggcaa aacctataac
     6481 tttggtcttt acccggcggg cgatgagtgg caactggcgc tcagtgacgg cgaaatcggc
     6541 aaaaattacc tctccgacgc gtttaaattt ggcggcgaac aaaaactgca actgaaagaa
     6601 acgaccgccc agcccgaagg cgaacgcgcg aatctacgcg tgattaccca gaatcgtcag
     6661 gcgctgagtg acattaccgc cattctgccg gatggcaaca aagtgatgat gagctccctg
     6721 cgccagtttt ctggcacgca gccgctctac acactcgacg gtgacggcac gttgacgaat
     6781 aatcagagcg gcgtgaaata tcgtccgaat aaccaaattg gcttttacca gtccattacc
     6841 gccgacggca actggggtga tgaaaagcta agccccggtt acaccgtgac tactggctgg
     6901 aaaaacttta cccgcgtctt caccgacgaa ggcattcaga aaccgttcct cgccattttc
     6961 gtctggaccg tggtgttctc gctgatcact gtctttttaa cggtggcggt cggcatggtt
     7021 ctggcatgtc tggtgcagtg ggaagcattg cgcggcaaag cggtctatcg cgttctgctg
     7081 attctgccct acgcggtgcc atcattcatt tcaatcttga ttttcaaagg gttgtttaac
     7141 cagagcttcg gtgaaatcaa catgatgttg agcgcgctgt ttggcgtgaa gcccgcctgg
     7201 ttcagcgatc cgaccaccgc ccgcacgatg ctaattatcg tcaatacctg gctgggttat
     7261 ccgtacatga tgatcctctg catgggcttg ctgaaagcga ttccggacga tttgtatgaa
     7321 gcctcagcaa tggatggcgc agatccgttc cagaacttct ttaagattac gctgccgctg
     7381 ctgattaaac cgctgacgcc gctgatgatc gccagcttcg cctttaactt taacaacttc
     7441 gtgctgattc aactgttaac caacggcggc ccggatcgtc ttggcacgac cacgccagcc
     7501 ggttataccg acctgcttgt taactacacc taccgtatcg cttttgaagg cggcgggggt
     7561 caggacttcg gtctggctgc agcaattgcc acgctgctct tcctgctggt gggtgcgctg
     7621 gcgatagtga acctgaaagc cacgcgaatg aagtttgatt aagggagata acaaaaatgg
     7681 caatggtcca accgaaatcg caaaaagcac gtttgtttat tactcacctg ctactgctac
     7741 tttttatcgc ggcgattatg ttcccgctgc tgatggtcgt cgctatctca ctgcgccagg
     7801 ggaactttgc gatcggcagc ctgatcccgg agcaaatctc ctgggatcac tggaaactgg
     7861 ctttaggttt tagcgtggaa caggctgatg gtcgcattac gccaccgcca ttcccggtac
     7921 tgctgtggct atggaactcg gtaaaggtcg ccgggatttc cgcgattggc attgtcgcgc
     7981 tctctaccac ctgcgcctac gctttcgccc gtatgcgctt tccaggcaaa gcgacgctgc
     8041 tgaaaggaat gctgattttc cagatgttcc cggcagtact ttcactggtc gcgttgtatg
     8101 cgttgtttga tcgtctgggt gagtacattc cattcattgg cctgaatact cacggcggcg
     8161 taattttcgc gtatctgggt gggattgcgc tgcatgtctg gaccatcaaa ggctatttcg
     8221 aaaccatcga tagttcgctg gaagaagcgg ctgcgctgga tggtgcgaca ccgtggcagg
     8281 ccttccgcct tgtcctgttg ccgctgtcag taccgattct ggcggtggta ttcatcctgt
     8341 cgtttatcgc tgccattact gaagttccgg tcgcgtcgct gttactgcgt gacgtaaaca
     8401 gctacaccct ggccgtgggg atgcagcaat acctcaaccc gcaaaactac ctgtggggtg
     8461 actttgccgc cgctgccgtg atgtctgcat taccgatcac catcgtcttc ttgctggctc
     8521 aacgctggct ggtcaacggc ctgacggcag gtggtgtgaa aggttaaaga tgttgttctg
     8581 ccaatgttat gccgctgcac cctcaactta cgttatccca acttgtgact gttattcggc
     8641 gctccacgga gcgccttttt ttctttcgtc tgcaatctga atcgttcgcc ggttaatatt
     8701 tccatcatag agcttattat ttttacgtta tttgttttcc cacttacgat aattctcttt
     8761 cgtgctctga gtcacggcaa tagtattgtt ttcatcaatt tttgataatt atcacaatta
     8821 agatcacaga aaagacatta cgtaaacgca ttgtaaaaaa tgataattgc cttaactgcc
     8881 tgacaattcc aacatcaatg cactgataaa agatcagaat ggtctaaggc aggtctgaat
     8941 gaatacccag tataattcca gttatatatt ttcgattacc ttagtcgcta cattaggtgg
     9001 tttattattt ggctacgaca ccgccgttat ttccggtact gttgagtcac tcaataccgt
     9061 ctttgttgct ccacaaaact taagtgaatc cgctgccaac tccctgttag gattttgcgt
     9121 agccagcgct ctgattggtt gcatcatcgg cggtgccctc ggtggttatt gcagtaaccg
     9181 cttcggccgt cgtgattcac ttaagattgc tgctgtcctg ttttttattt ctggtgtagg
     9241 ttctgcctgg ccagaacttg gttttacctc tataaacccg gacaacacag tacctattta
     9301 tctggcaggt tatgtcccgg aatttgttat ttatcgtatt attggcggta ttggcgttgg
     9361 tttagcctca atgctttcac caatgtatat tgcggaactg gctccagctc atattcgcgg
     9421 aaaactggtt tcatttaacc agtttgcgat tattttcggg caacttttag tttactgcgt
     9481 aaactatttt attgcccgtt ccggtgatgc cagctggctg aatactgacg gctggcgtta
     9541 tatgtttgcc tcggaatgta tccctgcact gctgttctta atgctgctgt ataccgtgcc
     9601 agaaagtcct cgctggctga tgtcacgcgg caagcaagaa caggcggaaa gtatcctgcg
     9661 caaaattatg ggcaacacgc ttgcaactca ggcagtacag gaaattaaac actccctgga
     9721 tcatggccgc aaaaccggtg gtcgtctgct gatgtttggc gtgggcgtga ttgtcatcgg
     9781 cgttatgctc tccatcttcc agcaatttgt cggcatcaat gtggtgctgt actacgcgcc
     9841 ggaagtgttc aaaacgctgg gggccagcac ggatatcgcg ctgttgcaga ccattattgt
     9901 cggagttatc aacctcacct tcaccgttct ggcaattatg acggtggata aatttggtcg
     9961 taagccactg caaattatcg gcgcactcgg aatggcaatc ggtatgttta gcctcggtac
    10021 cgcgttttac actcaggcac cgggtattgt ggcgctactg tcgatgctgt tctatgttgc
    10081 tgcctttgcc atgtcctggg gtccggtatg ctgggtaccg ctgtcggaaa tcttcccgaa
    10141 tgctattcgt ggtaaagcgc tggcaatcgc ggtggcggcc cagtggctgg cgaactactt
    10201 cgtctcctgg accttcccga tgatggacaa aaactcctgg ctggtggccc atttccacaa
    10261 cggtttctcc tactggattt acgggtgtat gggcgttctg gcagcactat ttatgtggaa
    10321 atttgtcccg gaaaccaaag gtaaaaccct tgaggagctg gaagccctct gggaaccgga
    10381 aacgaagaaa acacaacaaa ctgctacgct gtaatcttcc tgtccagcac gccgcgccag
    10441 ttcggcgtgc tttcttttta ctcccgcttc agccgtttcg aattacacag ccacagagtg
    10501 atcaccagta acaggatcgc agccgagtag atcaacacat ccagtggcga tttatgatcg
    10561 acgatgatca agcgcacaat ggcggtgatc ccaatataga caaagtaacg tagcgggaag
    10621 tgaaaaccgg actgaaagta cttcacaatc agcgcgataa attcgaaata gagaaagtaa
    10681 accaccagtc cttctaccag ctcatatttg ctggtttgtt ctggcgcgaa cagcacatct
    10741 gccagatgca ccgtttcttt gccgaggaag acaaccaaaa tcaggccgag gcacagcagg
    10801 ccaagattga gtacggtctg caaaatggtg gagataaact ccacgcgcgg acgagagagt
    10861 gacgtcatag cagtacctcc ttcacagtgg cgagcttcct gtataacgca aaaatgtgac
    10921 ggagatctat attttttgtg tatgttttgt tcaacctgga tttggagcgc gcctccacca
    10981 aagccggata tacctacccg taggccggat aagccgcgcc tggcggcgca tccggcaaga
    11041 caaaccgcac ccaagccggt aattcaaaaa tcacccattc aggtgattcc cagccaccag
    11101 caatgcgcga aaaatcgaca atgttcctct ggaacaaatg aaacgtaacc tgataaaggt
    11161 ggtaaaaatg aagccttatt ttgctgcttt gatgttatca gtctctgttc ttcctgctta
    11221 tgctggtccg ctcggtactg ctgataaagc cgacctaccg caaagcaatg tctccagccc
    11281 aatgatggcc cagtcgctca ggcaaccaga cctgcaaccg atatcgacgg acaggaagac
    11341 agaatgtttt cgcctttaca caccagatcg caaaccgggg gtgaactgcg ttcctgatgg
    11401 ctcaacaggc cattaagaat gagggtgcca gtgattgcga aaactggcac ctttcccatg
    11461 acttagcgga aattgacgct acggtcgctg gtcatatcat acaactggaa cttacgccca
    11521 agttgctgac caccgtcacg cgtcagcggc gtccagccaa tcgccgcacg gctgcgtgtc
    11581 gggccagacg agaagagatc caacggtacc gagacataca cgcctttggt gaagtcccct
    11641 tcgccgtact cctcttttga aacattcgtg atcgtggcat agccacccac caccacgccg
    11701 ctatcaaagc gtttggcgat ctccagcgtg ccgcctttat cccctgccag atactgcccg
    11761 acgctggctt tcactaacac gtcctgggcg aaagatggcg tccagtaggc ggtcagatgt
    11821 ccggttttca cgctgtagtc ggtgaatttc atcatatctt ttgcactacg ccagtcgcgc
    11881 tgtttaacgt agttggcatc cagaccaaac gcccagttgc tgtccagcgg acgatacaac
    11941 acttcagccc ccgcaccgcc aaacatggtt tcgagataac ctccatagac ctgaccgtag
    12001 aagccgttgc ccagatgctg gaagtagttg gcttgcaggt tattcacata ggcatcgttc
    12061 tgcacatact cgcgcacatg ggtacgaacg cgcggtaagt gcgagtcctg tggcggattg
    12121 gtgtagttaa acttgtcgta gttgttggca agatttgcaa acaggctgcc gatggtcagc
    12181 agatggtccg tcagccacaa atctgccgtt cccatcacgc ccagctgata catgtaaaag
    12241 ttttccgggc caccgaccga ctggttcagc accggatcga tatggaaatc aaagcgtgat
    12301 ttgtcgatat accagccctg ctcggtggac tgcggaacca ctggctcgac gcgtttttgc
    12361 gccagcgtcg tttcgtggcc caacggctct ccggcgagat gatttttcag gctggcgaca
    12421 tcggtttccg tcgtcacctg cggcatgtta aggcgatttt ccgtaatgcg gatcgttttg
    12481 atcccatccg gcagatcgtt catcacgatc cgattagcac ggatgatccc ttcgcgcgaa
    12541 tcacgatatt tcacctgctc gccggtaaca tacagcgtat cgcctttcgc ctggatctgc
    12601 ggatcggcaa gtccggcatt gtatttcagc aacgttaact gattcgccac caccgagtgc
    12661 tgcaaaatgg catcctgcgg ctgcggttga tattgcgggc gggcgttatc gatgtaggac
    12721 gggcgcagat cgttaaagtt ggtgcgcagc gtgacgccaa acataaaggt gttgccacgt
    12781 tcatagctaa ggttaacgtc ggcccaatcg gtaacgcgat aaatcgcccc gacgttaaac
    12841 ttactttttt gctccagctt cccggcaaaa tcctgctgat aattattacc ttcatactcc
    12901 agtttcaggc gcagcggttg ccagggcgtc tggtattcca cgccgccaaa cagtgaggcc
    12961 gggccgtgga acatctgact gccgtcgata gatcccgctt gtttgtagct gttatcgcga
    13021 tagcaatatt tatcactggc tgaacagagc ggatttttca cattaccgct ggtgcccaga
    13081 tatccccagc ccaggccgag cgtaaaatcg aacggccccc aggctttgct ggcaacaaga
    13141 tattccgcat caaacagccc cgtaccgccg atatcccgcg caccaaccgc cacttgcggc
    13201 agccagtaac tctcttccca caaacgcagt ttgagatcga aggctttatc tttatacgtt
    13261 tgatcgccag agaacgcttc gacgctgctg tactgccggg tgcgcacgtc ggtgtaacgc
    13321 agcgttgttt ccagccacgg gaagagttgc actgaagctg agtaataacg gtactgatcg
    13381 ttatcgcgat agttcagact caactcccct tcccgtgcca tacgcgcggt gggagtttgt
    13441 aataatccta cgccaccgaa atccgactgc gacggaccaa tgggcgcagg atatgtttcg
    13501 ccgtagcaag ctgtgctaat gcccagcgcc agtaagctaa gcagatgtct ttttttcatt
    13561 attggggtat ccgctgcgtc agagtctgaa gaatgtcggc gttaagggca tcaggcgtct
    13621 cactccagac ggagtctgcg aggccaacat aaataatgct gccgggcatc ggctctacgt
    13681 gacgcttgtt ccagtaagcc accggcgctt tttgcgtgcg tccgtccggg taaacgaccc
    13741 acgcgtagct gcgatccgca ccgctgagca ggctctggtc agagagatag ctcgccacgt
    13801 cgcgacctgg agtgaatggc tgattgccag gatggctgat aagcccgaac aacgtgaccg
    13861 tggacggcgg tggtccgacc cacagcgtgt agttgccctg caacggcggg ttaccgcgtt
    13921 cggcaacgcg gacgatatcg ggatcaagat tgattttttg cctgcctgtc acttccaatg
    13981 cctggatttg ctggcgtaag gcgttaatgg cggcggcatc gtcggtgctg gaatctgcgc
    14041 cctgttctgc cagccgtgtc agcaacgcct gctgctgacg caatgctgcc gccgttgcca
    14101 gctcttcgct aatcaccgca ccaggccacc agctgtttgc cagtcgaggt tgccctacca
    14161 gatcgattaa atgctctgcg cccgttagcg ttttggcctc actgctgcca ttgctgaaca
    14221 ccttgacggt tcctgccgcg aagaccgatg acgctcccac actcaaaagc aacgcgacaa
    14281 tagtttgttt aatcattgtt ttgccgcctt gatgagagtg gttttcaccg ggaaataatc
    14341 cgcgccgaga tactgttccg actggcgaat ttgcccttcg ctgtcgatcc agtagcggtt
    14401 atgccagcgc gcctggtcgg tggagacttc ttcgtccagc acgcgaacca gggtttcatc
    14461 gctgccgacc ttgacggtat cggtgccatc ccatttgaag actgagcgtg cggtggcgta
    14521 gcgtacctgc tggtactcgg tccagcccat cgtgcgcgtc catgttgcac catcaacaat
    14581 ttgtgcgggt ttaatcagcg ggtcggcggc aaggttattc acttcaatca ggttgtcgcc
    14641 gccaagcaaa gtcttcacca gacgaccatg ttgtgtgaca agagtggctt gatcctgagt
    14701 gacccatttt tgttgtccgt cttcagcgaa ggccagcacc acaaacaatt gcggcccgcc
    14761 attaagctgc atgtactggc tggcgtaggg catattttga atatcatcgt ccgtcagctg
    14821 tacgcctggc gtgccgaaca gactgtccca cagtgaattg cccagctctt tagtcgtggc
    14881 tgaacaggcc tgtaataaca ggcagataag aatgagtgca ggtcgcttca cgactcttct
    14941 ccagaggggc agaataacca caccggagtg tggttatggt taatcccttc atatttcaag
    15001 ccacagatgc aatgactcga attattttgg ggatacatac tttattactg ggtactggtg
    15061 gttgtggttg ttgtggtccc ggtattagaa ccatcaccgc caccggttgc cgcgagcgcg
    15121 acacccaccg ccgagcttac ggtgctgacg ctggtcgcgg aggaactccc cgccgaaacc
    15181 gacgttgctg ccgcccctgc cgcttcgccc acctgtacag gtgcagccca cacagaagtc
    15241 gccgcaagcg cagatatggc aaaaatgcca tacagaactt ttttcataac aatttccctt
    15301 cattgaatga atggagattt acccaaaaac atttcgggcg ggatcgagta tacacaacta
    15361 aagcatgcga attacggcgc ggggaattag cgatggtgtt ttaggataat tggaatgaat
    15421 atcaaatata agtttcaact tatatttaaa taacaataaa aatcataaac ttaaaataaa
    15481 aacaaaagta attaatctgc gtattctccg aaataaacaa cgattaaaaa tctattttag
    15541 gattaacctc acggtatgat ttccgttaaa tcacagacaa ggggatttat ctgataaaac
    15601 aatgccgact tattgtcggc attgttttta aggttattta actcctcata ctatgcatta
    15661 atgtcgatat tggcatcagg ctccggttgc cggatgcggc gtaaacgcct tatccggcct
    15721 acatatcggc atcaggcacg catgatgatt aaccgcgcca cgctttatag cggttaatca
    15781 gaccattggt cgagctatcg tggctgctga tttctttgtc atctttcagc tctggcagaa
    15841 tacggttcgc cagttgttta cccaactcca cgccccactg gtcgaaggta aagatgttca
    15901 ggatcacgcc ctgagtaaag attttgtgct catacagcgc aatcaacgca cccaggctga
    15961 acggagtgat ttcgcgcagc aggatggagt tggtcgggcg gttaccttcg aacactttga
    16021 acggcaccac gtagtcaagc gttgccggat ctttaccctg atcgcgatat tcctgctcaa
    16081 ccacttcgcg ggatttacca aacgccagcg cttcggtctg ggcgaagaag ttagacagca
    16141 gtttctggtg gtgatcagag agcgggttat gggtgatagc cggggcgatg aaatcgcacg
    16201 gtaccatttt ggttccctgg tggatcagct ggtagaacgc gtgctgaccg ttagtgcctg
    16261 gttcacccca gataatcggg ccagtctggt aatccacaac gttaccgtta cggtcaacat
    16321 acttaccgtt ggactccata ttgccctgct ggaagtacgc cgcgaaacgg tgcatatact
    16381 ggtcatacgg cagaatcgct tcagtttccg caccaaagaa attattgtac cagatgccaa
    16441 tcagcgccag caggacaggc aggtttttct cggcaggcgt ggtggagaaa tgcttgtcca
    16501 tcgcgtgtgc gccagaaagc agttcaacga agttatcaaa gccgatggag agaacaatcg
    16561 acaggccaat cgctgaccac aaagagtaac ggccgccgac ccagtcccag aactcgaaca
    16621 tgttggcagt atcaatacca aactcgccaa cggctttggc attggtggaa agcgccgcaa
    16681 agtgttttgc aacgtgtttt tcatcacctg ccgctttcag gaaccagtca cgcgcgctat
    16741 gggcgttggt catagtttcc tgagtggtga aggttttaga tgctaccagg aacagcgtgg
    16801 tttccgggtt tacttttttc agcacttcag cgatgtgagt cccatcgacg ttagaaacaa
    16861 agtgcatgtt caggtgattt ttgtacggac gcagtgcttc ggtcaccatg tacgggccga
    16921 ggtcagaacc accgatcccg atgttcacta cgtcagtgat tgctttgccg gtataaccct
    16981 tccactcacc ggagataatc gcttccgaga aggttttcat cttctccagc accgcgttga
    17041 cttccggcat tacgtctttg ccatcaacca gaatcggggt attgctacgg ttacgcagcg
    17101 ctacgtgcag cacggcgcgg ttttcagtgc ggttgatctt ctcgccagag aacatcgact
    17161 taatcgcgcc cgccagatcg cactctttcg ccagatcctg taatttcgcc agcgtctctt
    17221 cagtgatgcg gtttttggag taatccacca gcatctgatc gtcgaatgtt gcggagaact
    17281 tagaaaaacg atcgccgtct ttagcaaaaa gatcggcgat cgtaacgtct ttcatttcat
    17341 cgaagtgttt ctgtagtgcc tgccaggcag cggtctgcgt tggattgatg tttttcatta
    17401 gcaatactct tctgattttg agaattgtga ctttggaaga ttgtagcgcc agtcacagaa
    17461 aaatgtgatg gttttagtgc cgttagcgta atgttgagtg taaaccctta gcgcagtgaa
    17521 gcatttatta gctgaactac tgaccgccag gagtggatga aaaatccgca tgaccccgtc
    17581 gttgacaacc gccccgctca ccctttattt ataaatgtac tacctgcgct agcgcaggcc
    17641 agaagaggcg cgttgcccaa gtaacggtgt tggaggagcc agtcctgtga taacacctga
    17701 gggggtgcat cgccgaggtg attgaacggc tggccacgtt catcatcggc tacaggggct
    17761 gaatcccctg ggttgtcacc agaagcgttc gcagtcgggc gtttcgcaag tggtggagca
    17821 cttctgggtg aaaatagtag cgaagtatcg ctctgcgccc acccgtcttc cgctcttccc
    17881 ttgtgccaag gctgaaaatg gatcccctga cacgaggtag ttatgtctga aattgttgtc
    17941 tccaaatttg gcggtaccag cgtagctgat tttgacgcca tgaaccgcag cgctgatatt
    18001 gtgctttctg atgccaacgt gcgtttagta gtcctctcgg cttctgctgg tatcactaat
    18061 ctgctggtcg ctttagcgga agggctggaa cctggcgagc gattcgaaaa actcgacgct
    18121 attcgcaaca tccagtttgc cattctggaa cgtctgcgtt acccgaacgt tatccgtgaa
    18181 gagattgaac gtctgctgga gaacattact gttctggcag aagcggcggc gctggcaacg
    18241 tctccggcgc tgacagatga gctggtcagc cacggcgagc tgatgtcgac cctgctgttt
    18301 gtcgaaatcc tgcgcgaacg cgatgttcag gcacagtggt ttgatgtacg taaagtgatg
    18361 cgtaccaacg accgatttgg tcgtgcagag ccagatgtag ccgcgctggc ggaactggcc
    18421 gcgctgcagc tgctcccacg cctcaatgaa ggcttagtga tcacccaggg atttatcggt
    18481 agcgaaaata aaggtcgtac aacgacgctt ggccgtggag gcagcgatta tacggcagcc
    18541 ttgctggcgg aggctttaca cgcatctcgt gttgatatct ggaccgacgt cccgggcatc
    18601 tacaccaccg atccacgcgt agtttccgca gcaaaacgca ttgatgaaat cgcgtttgcc
    18661 gaagcggcag agatggcaac ttttggtgca aaagtactgc atccggcaac gttgctaccc
    18721 gcagtacgca gcgatatccc ggtctttgtc ggctccagca aagacccacg cgcaggtggt
    18781 acgctggtgt gcaataaaac tgaaaatccg ccgctgttcc gcgcgctggc gcttcgtcgc
    18841 aatcagactc tgctcacttt gcacagcctg aatatgctgc attctcgcgg tttcctcgcg
    18901 gaagttttcg gcatcctcgc gcggcataat atttcggtag acttaatcac cacgtcagaa
    18961 gtgagcgtgg cattaaccct tgataccacc ggttcaacct ccactggcga tacgttgctg
    19021 acgcaatctc tgctgatgga gctttccgca ctgtgccggg tggaagtaga agaaggtctg
    19081 gcgctggtcg cgttgattgg caatgacctg tcaaaagcct gcggcgttgg caaagaggta
    19141 ttcggcgtac tggaaccgtt caacattcgc atgatttgtt acggcgcatc cagccataac
    19201 ctgtgcttcc tggtgcccgg cgaagatgcc gagcaggtgg tgcaaaaact gcatagtaat
    19261 ttgtttgagt aaatactgta tggcctggaa gctatatttc gggccgtatt gattttcttg
    19321 tcactatgct tatcaataaa cgagcctgta ctctgttaac caacgtcttt atcgagaata
    19381 attgccttta atttttttat ctgcatctct aattaattat cgaaagagat aaatagttaa
    19441 gagaaggcaa aatgaatatt atcagttctg ctcgcaaacg aattcccgta cactgaactc
    19501 tctggctcac tgagggcacc atcatggcac tcccccgtat tacccaaaaa gagatgactg
    19561 aacgcgagca gcgtgaactt aaaacactgc tggatcgggc gcggattgcg catggtcgcg
    19621 tactgaccaa ctccgaaacc aacagcatta agaaagagta catcgataaa ttaatggtcg
    19681 agcgtgaggc tgaagcgaaa aaagcccgcc aattgaagaa aaagcaggct tataaaccgg
    19741 atcctgaagc gtcatttagc tggtcagcaa atacgtcgac gcgcggcagg cgctaatgaa
    19801 caatcaacgc ctgagatttt cactcgtctg tgtgaacaca attttcccct caccctaacc
    19861 cttttcccga aggggcgagg ggactgtccg agcacttttg ggccgttttc atcagtctga
    19921 ggcgttaatc agcgcccctt ctttttacgc cccggcgagg taaaacgctt accgttggac
    19981 gccgggcgac cgcctttttc cgccgttttt tccatcttaa cgactggacg tttgatgccc
    20041 gctgttttcg gtttggcctt cgctttgggc ttcgcctcgg aagaggaatt ttcaatgagc
    20101 ttaaagagat cgataagctc gtcgtcggtt aaatcgcgcc attctcccag cggaatgccg
    20161 cttaagctga cgttcataat gcgcgtacgt tccagctttt tcacttcata gccgaaatgc
    20221 tcgcacatgc gccggatctg acggttcaac ccctgcacca gggtaatgcg gaagacaaac
    20281 ggtgcttctt ttttgacttt gcactttttc gtcaccgtcc cgaggatcgg cactcccgcc
    20341 cccataccac gaataaattc atcggtaatc ggtttatcga ccgtcaccag atactctttc
    20401 tcatgatcgt tacccgcacg gaggatctta ttcaccagat cgccgtgatt ggtgaggaaa
    20461 atcagcccct gggagtcttt atccaggcgg ccaatcggga acacgcgttt gctgtggtta
    20521 acgaaatcga caatgttatc gcgctcgcca tcttcggtgg tgctgacaat accgacgggc
    20581 ttgttcaggg cgataagtac caaatcttcg gcttcccgag gctcaatcaa ctgaccgttt
    20641 actttcacaa tgtcgccggg tttcacctga tcaccaatgg tggctcgctt gccattaagg
    20701 aacacattgc cttgctcgat atagcgatcc gcttcgcggc gtgagcaaat tccgctttcg
    20761 ctgatgtatt tatttaaacg gactgatgag tcgggcagca taaattctcc tgtaaaacgc
    20821 ggaatatacc gtacctctta gcggggaaaa acagggttat gcggcaacgt cgaaattaaa
    20881 tgtgatctgc cacgcctttt atcacaaaag tgtctccgga agtacgatgc aaatttgcct
    20941 ggcaaaataa ggataatcaa catattacga atcgctgcac acctgtgctg atgactacta
    21001 cgcagagaaa caaatgtgca agagaaagcg ctatttctcc ccatgcaaag agtgaaaaat
    21061 cgctttgaaa ggccctcata atggggttat atcagatcaa gcggtgagaa agcgtatttg
    21121 cacaaatgtg cagaaccagg aggcggcaat ggaaaacagt gacgatatcc gtttgattgt
    21181 gaagattgcc caactctatt acgaacagga tatgacgcag gcgcaaatcg cgcgcgaact
    21241 gggtatttac cgcaccacca tcagccgctt gcttaaacga ggccgcgatc agggaattgt
    21301 caccatcgcc atcaactatg actacaacga aaatctctgg ctggagcagc aactgaagca
    21361 aaagtttggc ttgaaagacg ttgtggtggt gtcgggaaat gatgaggatg aagagactca
    21421 actggcgatg atggggttac acggcgcgca actgctggat cgcttgctgg agcctggcga
    21481 tattgtcggt ttttcctagg gtcgcgcggt gagcgcactg gttgaaaact tgccgcaagc
    21541 ggggcaatcg cggcagttaa tctgcgtgcc aattattggc ggaccgtccg gtaaactcga
    21601 aagccgctat cacgtaaaca cattaaccta cagcgtggca gcgaagctga aaggggaatc
    21661 gcatctcgcg gattttccgg ctctgctgga taacccctta attcgtaatg ggatcatgca
    21721 gtctcagcac tttaaaacca tctctgccta ctgggataat ctggatgtcg ccctggtggg
    21781 aattggctca ccggccattc gcgacggcgc taactggcat gcgttttatg gtggtgaaga
    21841 gagtgacgac ctgaaagcag gcgcggtatt ccattggcat tgccatgagt gaagaaaaat
    21901 acagcggaat tgttggtgca ctgcgtggaa aatatattaa ttgtctggta acgaatagca
    21961 gcacagctga actattactg aaataaaaat cttcccgatg tttaccgtaa taaaaattac
    22021 ggcgggtccc tattatctaa aatcaggagt ctgttatgca aacgtggtta aatttgcagg
    22081 ataaaatcat tatcgtcacc ggcggcgcat ccggtattgg tctggcgatt gtagaggaat
    22141 tattagcaca aggcgcaaac gtacagatgg tagatattca cggtggcgat ggtcaatatg
    22201 aagttcataa aggctatcag ttttggccga ccgatatttc cagcgccaaa gaggtaaatc
    22261 atacggtagc ggaaattatc cagcgttttg gtcgcatcga cggtctggtc aataacgccg
    22321 gggtcaattt cccgcgtctg ctggtcgatg agaaagcgcc tgccgggcag tatgaactca
    22381 acgaagctgc attcgaaaaa atggtcaata tcaaccaaaa aggcgttttt ctgatgtcgc
    22441 aggcggtggc gcgccagatg gtcaaacaac atgatggcgt gattgtgaat gtttcctcag
    22501 aaagtggact ggaaggctca gaaggccaaa gctgttacgc cgctaccaaa gccgcgctca
    22561 atagcttcac gcgctcctgg tcgaaagagc tgggtaaaca cggtatccgt gtggtcggta
    22621 tcgcgccggg gattctggaa aaaaccgggc tacgcacgcc ggaatatgaa gaggcgctgg
    22681 cgtggacgcg caatatcacc gtcgagcagc tgcgtgaagg ctataccaaa aacgccattc
    22741 ctattgggcg cgccggaaga ttagcagaag tcgctgattt tgtttgttat ctgctgtctg
    22801 aacgcgccag ctacatcacc ggagtaacca ctaacattgc gggcggcaaa acgcgcggat
    22861 aaggaggctt tatggtcaac gccatttttt gtgcccacgg caaattagct tgcgccatgc
    22921 tggaatcggt tcagatggtg tacggcgacg ccaatgtcga ggcggttgaa tttgtaccag
    22981 gcgagaacgc cggagacatc gtagcaaagc tggaaaagtt agtaagcatt cacaatcaag
    23041 atgagtggct gattgccgtg gatttacagt gcggcagccc gtggaatgcg gcagcgatgc
    23101 ttgcgatgcg caatcctcgt ctacgggtaa tcagcggcct ttccctgccg ctggcgctcg
    23161 aactggttga taaccaggac agcatgaatg ttgatgaact gtgtgaacac ctgacgcaaa
    23221 tcgcaaagca aacctgcgtt gtctggaaac aactggcaac ggcagaggaa gatttctgat
    23281 gaacattacg ctcgcccgca ttgatgaccg tctgatccac ggccaggtca ctaccgtctg
    23341 gtcgaaagtg gctaacgccc agcgcattat tatctgcaat gacgaagttt ataacgatga
    23401 agtgcgccgg acattattgc gtcaggctgc gccaccggga atgaaggtta acgtggtcaa
    23461 tattgaaaaa gccgtcgcgg tttatcacaa cccgcaatat taggacgaaa ccgtttttta
    23521 tcttttcact cgcccacagg atgccttagc gatggttcgc cagggggtaa aaataggcac
    23581 cctgaatatt ggcggcatgg cctggcggcc cggtaaaaaa cagttaacca aagcggtttc
    23641 attagatgat gatgatatta acgcatttca tgagttaaat aatcttggcg tcataatgga
    23701 tttacgcgtc gtagcgtcag atccctcaat caatattatc gacaaaataa acgaacagtt
    23761 aatcgcaaat taaataatag cgcctgtgta catatgcttt aacaggcagg gatgacgaca
    23821 cttaaaaggt gacaaatcat ggaaataagt accctacaaa ttattgctat atttcttttt
    23881 tcctgtattg ccggaatggg cagcgtgctg gatgaatttc agactcatcg cccgttaatt
    23941 gcctgtacgg tgattggttt aattctcggt gatttaaaaa ccggaataat actcggtggt
    24001 acgctggaat tgatagctct cggctggatg aacgtcggcg cggcgcaatc tccggattct
    24061 gcactcgcca gcataatctc cgccattctg gttatcgttg gtcagcaaag catcgccacc
    24121 ggaatcgcca tcgcgttgcc tgtggctgcg gcaggccaag tgctgacggt gtttgcccgt
    24181 accatcaccg tagtgttcca gcacgcggcg gataaagcag cggaagaggc gcggtttcgc
    24241 actctcgata ttctgcatgt ctccgcgctt ggcgtgcagg cgctgcgcgt tgctattccg
    24301 gcactgattg tctcactgtt cgtcagcgcc gatatggtca gcaatatgtt aaacgccatt
    24361 cccgaatttg tgacccgtgg actgcaaatt gctggcggtt ttatcgtggt ggtcggttac
    24421 gccatggtgc tacgtatgat gggcgtgaaa tatttgatgc ctttcttttt cctcggtttc
    24481 ctcgcaggtg gctacctcga tctcagcctg ctggcgttcg gtggcgtcgg cgtgatcatg
    24541 gccctggtct acatccagct aaatccacag tggcgtaaag ctgaacctca actccaggcc
    24601 accgcctcca ccgcccttga ccaacttgac gattaacgga gccatcatgg aacagagaaa
    24661 aattacacgc agcgatctgg tgagcatgtt tctgcgctcc aacctgcaac aggcgtcctt
    24721 taactttgaa cgtattcacg gactgggctt ttgctacgac atgatccccg ccatcaagcg
    24781 actttaccca ttaaaagagg atcaggttgc ggcgctcagg cgacacctgg tgttcttcaa
    24841 taccacgcca gccgtatgtg gcccggtcat cggcgtcact gccgccatgg aagaggcgcg
    24901 ggccaacggc gcggaaattg atgacggtac cattaacggc atcaaagtcg gtctgatggg
    24961 accattggca ggagttggcg atccactggt ctggggaacg ctgcgcccga ttaccgctgc
    25021 gctcggcgca tctctggcac tttctggcaa catcctcggc ccgctgctgt ttttctttat
    25081 tttcaatgcg gtgcgtctgg cgatgaagtg gtatggccta cagctcggct ttcgcaaagg
    25141 ggtgaatatc gtcagcgata tgggcgggaa tgtgctgcaa aaactcaccg aaggcgcgtc
    25201 gattctcgga ctgtttgtga tgggcgtgct ggtcaccaaa tggacgtcaa tcaacgtacc
    25261 gttggtggtt tcacaaacgc atgccgccga tggctccacc gtcatcatga ccgtgcagaa
    25321 cattctcgac caactttgcc ctggtttgct ggcgctcggt ctgacgctac tgatggttcg
    25381 tctgctcaac aaaaaaatta acccggtatg gctgattttc gccctgtttg gcttagggat
    25441 tatcggcaat gcgctgggct tcctgtcctg attcttcgcc ccggcacgac tgccggggcc
    25501 atcgctcaac atgaggtggt ttatgaaaac aacagctctg cgtctttatg gtaaacgtga
    25561 tttacgcctg gaaacctttg accttcctga aatgcaggag gatgaaatcc tcgcgacggt
    25621 ggtcactgac agcctgtgcc tctcttcctg gaaagaggca acctgggtga aaaccataaa
    25681 aaagtacccg acgatgtggc gaccaaccca atcatcatcg gccacgagtt ttgcggcgat
    25741 attctggccg tgggtaaaaa gtggcagcac aaattccagc cgggtcagcg ttatgtgatt
    25801 caggccaacc tgaaactccc tgaccgcccg gactgccccg gctactcctt cccgtgggtg
    25861 ggcggcgaag ccacgcatgt ggttattccc aacgaggtca tggaacaaga ttgcctgctg
    25921 gcatacgacg gcgaaaccta ttttgaaggc tcgctggttg aaccgctttc ctgcgtgatt
    25981 ggcgcgttca acgccaacta tcatcttcag gaaggtagtt ataaccacac gatggggatt
    26041 cgcccgcaag ggcgcacgct gatcctcggc ggcaccggac caatgggatt gttggcgatt
    26101 gattatgcgc tgcatggacc agttaacccg tcgctgctcg tcattaccga taccgacaac
    26161 gacaaattga gttatgcgcg caagcactat ccatcagaac cgcaaacact gattcattat
    26221 ctcaatgcca ccgatgcagc atttgatacg ttaatggcgc tgagtggcgg tcacggcttc
    26281 gatgatattt tcgtctttgt gcctaatgaa ggactggtga ctctcgcctc ttccttgctg
    26341 gcgacagatg gttgcctgaa tttcttcgcc ggaccgcagg ataaacattt cagcgcgcca
    26401 attaatttct acgatgtgca ttatgcattt acccactacg tgggcacgtc aggcggcaat
    26461 accgacgaca tgcgcgcggc ggtcaaactg attgaagaga aaaaagtgca ggccgcaaaa
    26521 gtggtaacac atattcttgg gctgaatgcc gcgggcgaaa ccacgcttga attgcctgcc
    26581 gtcggcggcg gcaaaaagct ggtgtatacc gggaaatacc tgccgctgac gtcactcacg
    26641 cagattcagg atcaagcact ggcggcgatt ctggcgcgtc atcaggggat ctggtccggt
    26701 gaggcggagc aatatctgct cactcatgca gaggcaattt cccatgatta atcgcgctac
    26761 ttaactctgc atgtcgctgg ctggtcgccg cggcaacttc gcgacctgat gtgcggctga
    26821 ttgctgaagc ggcccaatgt gcacgagaag gataaaaaaa catcagcgcc ttaaggggcg
    26881 ctcaaattgg tggcaaagta cacattgttt atgccggatg cggaataaag ccttattcgg
    26941 cctacataat ctcgaaaatt caatatattg cagttatctg gtaggcctgg taagcgtagc
    27001 gcatcaggca atgctgtgtt tgtcatcagt ctcagcgccc ggtgcaatac gtgacatcgt
    27061 gagccatgtc agtaagtggt taaattcgca gtggtagcat gccttccgtg atttcttact
    27121 aaccggagaa gtaatggaac tgcttttatt gagtaactcg acgctgccgg gtaaagcctg
    27181 gctggaacat gcactgccgc taattgctga acagttgcag ggtcgccgct cagcggtgtt
    27241 tatccctttc gctggcgtaa cgcagacctg tgatgattac acagcgaaaa cggctgcggt
    27301 tctcgctccg ctgggtgttt ctgtcaccgg tattcatagc gttgtcgatc ccgttgccgc
    27361 gattgaaaat gctgagatcg tgattgtcgg gggcgggaat actttccagt tgctgaaaca
    27421 gtgccgcgag cgcgggctgc tggcaccaat tactgacgtg gttaaacgtg gtgcgctgta
    27481 tattggctgg agcgcaggcg ctaaccttgc ttgcccaacc attcgtacca ccaacgatat
    27541 gccgattgtc gatccgcaag gtttcgatgc gctaaatctg ttcccgctgc aaatcaaccc
    27601 gcacttcacc aacgcgctgc cggaaggcca taaaggtgaa acccgtgagc agcgtattcg
    27661 cgaactgctg gtcgtcgcgc cagaactgac gattattggt ctaccggaag gtaactggat
    27721 cacggtgagt aaaggtcacg ctacgctggg tggcccgaac accacttatg tgtttaaggc
    27781 cggtgaagaa gcggtgccgc tggaagcggg tcaccgtttt taagtgaatt gccggatgca
    27841 tgatccggca tcttgttcat agtgcctgat gcgacgcttg acgcgtctta tcaggcctac
    27901 aggattcaca atattaatac tcgtcccgtc cttcatcttc atccggctgt tccagcacac
    27961 tgtaagccac cgaacaaaac agcgagttca gacgctgcat atctcccagt aagcctaaat
    28021 gtagcgaact ggtttcaatg ctctgcacgt tttgctgatg caggcgatcg acgtgggcgt
    28081 gggaatagcg gcgattaaga atgcgaaagc gatgcttgct gcggcgcaaa cgacgagcgc
    28141 tggtgacatc gccagagaag aacaccgaca ttgccagctt taaattactg agcaattgct
    28201 catagagcgc atccagttcc ttcaacccat caagcgaaaa tgcccgccgt gctgccagcg
    28261 atttatcagc aatttcgctg cccatgcgct cgacgatatc ggaagcctgt tcaaggttga
    28321 gcgacatttc aataatctcc gcccagcgac gcgactcttc ttctgccagt tcctcttttg
    28381 gcattcgcgc cagatacagc ttaatggcgg tgtagagaac gttgatatca tctgccagct
    28441 tacgcagctc tttctcctgc cgtggctcgc cgtgcatcac tttattcagc ccttccatca
    28501 tctgttccat ggcgtcgcca atgcgcaggg tttcgcgcgc ggcgttcgcc agagcaagcg
    28561 tgggcgtatc cagcgcgctg acatccagat gtttaggccg tagctgggta tccagttccg
    28621 gttcatcgcg aataatcgtt ttgcaaaacc gtgccatcgg gtcaacaaat ggcagcatga
    28681 ccaggcaacg tacaaggttg tagaagacgt ggaaatagat caccagttcc gcttttggca
    28741 atgacaactt ccccattgtc tctgccagca aatggacaaa cggcaggata atcaggctac
    28801 ccaccagctt aaacagcaaa ctacccagcg cgacacggcg ggctgcggca ttggcggcac
    28861 tgttgttgag catcgccagc aggccggaac cgaggttagc accaatcacc agacagagcg
    28921 ccacggggaa ggagataatg cctgcggcgg tcagagtcgc ggtcagcagt acagcagcaa
    28981 ggctggagta gctgataatg gcgaacatcg cgccaatcag cgcatccagc agaatatcgc
    29041 cggtcagcga ggcaaagatc acctgaacgc cgtttgcctg ggtgatcggc gttacggcct
    29101 gcacaatcaa ctccagcgcc agcaaaatca gcccaagacc aataccgacg cggcccagtt
    29161 gcccggcgcg tgactgtttg cgtccgagga aaaaaatcac gccgataaaa ataagtaacg
    29221 gtgacagcca ggataagtcg aaggtgagaa tacgcgccat tagcgccgtc ccgacatctg
    29281 cacccagcac aatcaccaga gccggtgcga gggctaccag atcctgagcg acgaacgagg
    29341 tcaccagcat ggtggtggca ttactgctct gtaccagtgc ggtaacgccg atccccgcgc
    29401 aaaaggcgag cggctttttt tcgacgctcc ggctaaggac agtacgcaaa cgcgcgccga
    29461 agacgcgcat tacgccggtt cgaacaatat gagtccccca gaccagcagg gcgacggcag
    29521 aaagcaggtg aagcagcgtt aacacgagtt caggttctcc ttatcgttat atgttcctga
    29581 agcaattgaa tgaccaaagg accggaaaca cgctgactgg catctttact gtgccttttg
    29641 acattcgttt ttccggctaa gattattcca gcctgtttcc agtataagtg tttcatatta
    29701 ataaagagac agggcactca ttgagcgccc tgtttgttgt aaggaaaagt gacagatttg
    29761 tgaatcagtc cgcgtcatac cccagattcg gtgccagcca gcgctcaaca tcggagacgc
    29821 tcataccttt acggcgggca tagtcttcaa cctgatcgcg ctgaatttgc gccaccgcgt
    29881 agtacttgct gtccgggtgg ctgaaatacc aacccgaaac cgatgcacca ggccacatgg
    29941 cgaaagattc tgtgagtttc atgccagtat gtttttccac ttccagcagc tcccagatgg
    30001 tggctttttc cgtatgttcc gggcaggccg ggtagcccgg tgccggacgg atgccctggt
    30061 agttttcgcg gatcagttct tcgttgctga ggttctcgtt cggcgcatag ccccagtaga
    30121 ctttacgcac acgctcatgg agatactccg caaaggcttc ggctaaacgg tcggcaagcg
    30181 ctttcaccat gattttgttg taatcatcgt gctgcgcttc aaaggcatca gccagtgcgt
    30241 cctcttccag cccgccagtc acggcaaatg cgccgatgta atctgcttta ccagaaagct
    30301 tcggcgcaac gaagtcagcg agacagtagt tagcgaatcc cgttttttcg gtctgttgac
    30361 gcagatggtg gctgacgttg atcacatggg tgcgcgtttc gtcacggtag atttcaatgt
    30421 catcgcccac acggtttgcc gggaacaagc ccaccacgcc acgcggattc agcgttttct
    30481 cggcgcttaa tttatccagc atgtcgttgg cgtctttaaa cagccgctgc gcctcaacgc
    30541 ccaccacttc atcttccaga atgcgcggat acttcccggc cagcgaccag gtcataaaga
    30601 acggtgtcca gtcgatgtaa ttacgcagag tttcgatgct ggcttcaact tcctgcacgc
    30661 cgagacggtg cgccaccggc ggcgtgtaag cctgccagtc aaaagcgaag tcgttatcgc
    30721 gcgccgcttc cagcgtgacc ggtggtgtgc gcggtttctt gcgcccgtgc tgaatacgta
    30781 cggtttcata ctccttgcgg gtacgagcga caaaatcatc gcgctgagta tcggaaagca
    30841 gcgccgccac cacaccaacg gtgcgcgagg cattctgcac atacaccgtc gggccgctgt
    30901 agttctgctc aattttcacc gccgtgtgcg cttttgaggt cgtcgcgcca ccgatcagta
    30961 acggaatggt gaagccctga cgctccatct ctttcgccac gttaaccatc tcgtccagcg
    31021 acggcgtgat aagccccgaa agtccaatca gatcagcatt cacttcttta gcggtacgga
    31081 gaattttttc cgcaggcacc ataacgccga gatcgacaat ttcgtagttg ttacattgca
    31141 gcaccacacc aacgatattt ttaccgatgt cgtggacgtc gcccttcacg gtggcgatca
    31201 ccatcttgcc gttggttttg ccctgctctt tgctggcttc aataaacggt tcgaggtagg
    31261 ccactgcctg tttcatgacg cgcgccgatt tgaccacctg tggcaggaac attttcccct
    31321 cgccaaacag atcgccgacc acgttcatgc cgtccatcaa cgggccttca atcacttcaa
    31381 tcgggcgcgt agcctgctgg cgggcttctt cggtatcctg ctcgataaac tcagtaatgc
    31441 ctttgaccag cgagtattcc agacgtttat tcacttccca cgagcgccac tccgcctgct
    31501 gggcgttagc ggtgtcgtcg gttttgctgc cgcgatattt ctcggcaagc tccagtaaac
    31561 gttcagtgcc atcatcgcga cgattaagaa tcacatcttc caccgcgtcg cgcagttcag
    31621 cgggcaggtc gtcgtaaatc gccagttgcc cggcgttgac gatccccata tccatgccat
    31681 tgcgaatagc gtagtagagg aacaccgcgt gaatggcttc gcgcaccggt tcgttgccac
    31741 ggaatgagaa agaaacgtta gatacgccgc cggaaatcag cgcgtgcggc agttcgcgtt
    31801 tgatgtcttc gcaggcgccg ataaagtcct gcgcatagtt gttgtgctct tcaatgccgg
    31861 tggcgaccgc gaagatgttc gggtcgaaga tgatatcttc tggcgggaag ccaacctctt
    31921 cggtgaggat tttgtacgcc cgacggcaaa tctcgatttt ccgtgcgcgg gtatcggcct
    31981 gtccctgttc gtcaaaggcc attaccacca ccgctgcacc gtaacgacgc aacagtttcg
    32041 catgatggat aaaggcgtcg acgccctctt tcattgagat agagttaaca atgcctttgc
    32101 cctgaataca cttcagcccc ttttcgatga cctcccattt tgaggagtcg atcataatcg
    32161 gcacgcgagc gatatccggt tcaccggcaa tcagattgag aaaacgcacc atcgccgctt
    32221 cggcgtcgag catcccttca tccatattga tatcgataat ctgcgcgccg ttttccacct
    32281 gctggcgggc cacatccagt gcctcgctgt atttctcttc tttgatcagg cgcttgaact
    32341 tagcggaacc ggtgacgttg gtgcgttcac ccacgttcac aaacaggcta tcttcgccaa
    32401 tgttcagcgg ctccaggccg gacaaacggc aggctacggg gatttccggc agtttgcgcg
    32461 gcgctaatcc ttctactgca cgactcatcg ctgcaatatg ttgtggcgtg gtgccacagc
    32521 agccgccgac gatattgaga aagcccgctt ccgcccattc acgtatttgt tttgccatcg
    32581 tgtcggcgtc gagatcgtac tcaccaaagg cgttgggtag cccggcgttc gggtgcgcgg
    32641 tgacgtagca ttccgcaatc cgtgacagct cctgcacgta ctggcgcagt tcatcgggcc
    32701 ccagcgcaca gttcaggcca aaggtcagcg cttcggcgtg gcgcaatgag ttgtaaaatg
    32761 cttcggtggt ctgcccggag agcgtgcgcc cggaggcgtc ggtgatggtg ccagagatca
    32821 taatcggcag ctcaacgccc agcgtttcaa actccgtttt taccgcaaat accgccgctt
    32881 tggcgttaag ggtgtcgaaa acggtttcaa tcaggatcag atccgcacca ccttccacca
    32941 gcgctttggt ggactctcga taagccacca ccagcccgtc aaaagtgata ttacgaaatg
    33001 ccggatcgtt gacgtccgga gaaatagacg ccgtgcggtt ggttgggccg agaacaccgg
    33061 caacgtagcg cggtttctcc ggcgtgcgcg cggtccactc gtcagcacag gcgcgcgcca
    33121 gtttcgccgc cgcaaagttg atttccgccg acagggattc catctggtaa tccgccatcg
    33181 caatggtcgt ggagttgaag gtgttggttt cgatgatatc cgcgcccgct tcaaagtagg
    33241 cgttgtggat agcggcgatc acttccggtt tactgagtac cagcaggtcg ttgttgcctt
    33301 tgaggtcgca tggccagtcg gcaaagcgtt caccacgaaa atcggcttcg ttcagtcgat
    33361 agctctggat catggtgccc ataccgccgt ccagcaccag aatacgttca tttaactgcg
    33421 cacgcagttg ttccactttg ctgctcacac ttgctcccga cacgctcaac ccagatttaa
    33481 taaaaattca acaaaccata ctggcataaa cgcatctgtg gtaaaagcga ccaccacgca
    33541 acatgagatt tgttcaacat taactcatcg gatcagttca gcaactattg cattaactaa
    33601 caataaaaat gaaaatgatt tccacgatac agaaaaagga gactgtcatg gtcgcaccca
    33661 ttcccgcgaa acgcggcaga aaacccgccg ttgccaccgc accagcgact ggacaggttc
    33721 agtctttaac gcgtggcctg aaattactgg agtggattgc cgaatccaat ggcagtgtgg
    33781 cactcacgga actggcgcaa caagccgggt tacccaattc cacgacccac cgcctgctaa
    33841 ccacgatgca acagcagggt ttcgtgcgtc aggttggcga actgggacac tgggcaatcg
    33901 gcgcacatgc ctttatggtc ggcagcagct ttctccagag ccgtaatttg ttagcgattg
    33961 ttcaccctat cctgcgcaat ctaatggaag agtctggcga aacggtcaat atggcggtgc
    34021 ttgatcaaag cgatcacgaa gcgattatta tcgatcaggt acagtgtact cacctgatgc
    34081 gtatgtccgc acctatcggc ggtaaattgc cgatgcacgc ttccggtgca ggtaaagcct
    34141 ttttagccca actgagcgaa gaacaggtga cgaagctgct gcaccgcaaa gggttacatg
    34201 cctataccca cgcaacgctg gtgtctcctg tgcatttaaa agaagatctc gcccaaacgc
    34261 gcaaacgggg ctattcattt gacgatgagg aacatgcact ggggctacgt tgccttgcag
    34321 cgtgtatttt cgatgagcac cgtgaaccgt ttgccgcaat ttctatttcc ggaccgattt
    34381 cacgtattac cgatgaccgc gtgaccgagt ttggcgcgat ggtgattaaa gcggcgaagg
    34441 aagtgacgct ggcgtacggt ggaatgcgct gactttttct ggcgggcaga ggcaatattc
    34501 tgcccatcat acctgagtgg caatagaata agggtgtctg ttaatcgcat tgacgccaaa
    34561 ataacttaat gtcatacact tcacacactt ttattaactc agcattattt ttaaacatca
    34621 aaccacttaa ttattaacaa aatcgcaaga tctcattcca ccgaaacttt tacggctgca
    34681 ttatgagatt gagcgattta tgatatttct ttagttgtaa tcatattaaa ttttttcact
    34741 gttctgatag ttaaaattta agacatcaat aaacaatgag atatttaaat gattactcgt
    34801 attcctcgta gttctttctc tgcaaatatt aataatacag cccagacaaa tgaacaccaa
    34861 accctgagtg aattgtttta taaagaactc gaggataaat tttctggcaa ggagctggcg
    34921 acgcctctat taaaaagctt ctcagagaac tgtagacaaa atggtcgtca tatctttagc
    34981 aacaaggatt ttgtcattaa attttccacg tccgtcttac aagctgataa gaaagaaatt
    35041 acgataatta ataaaacgaa aacacgacac tcactcaaac cattgctcca atatttgaag
    35101 aatacctaat ggaaatttta cctcaacgct cagacactct tgataaacaa gaattaaacc
    35161 taa
//
LOCUS       DADMKF010000028        34981 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.028, whole genome shotgun sequence.
ACCESSION   DADMKF010000028 DADMKF010000000
VERSION     DADMKF010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34981
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.028"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(<1..815)
                     /locus_tag="JNC24_001233"
     CDS             complement(<1..815)
                     /locus_tag="JNC24_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HAY9158037.1"
                     /translation="MKPAHNPSFFRSFCGLGCISRLSVEEQNITDYHRIWDNWAKEGA
                     ATEDRTQAVRLLKICLAFQEPALNLSLLRLRSLPYLPPHIQELNISSNELRSLPELPP
                     SLTVLKASDNRLSRLPALPPHLVALDVSLNRVLTCLPSLPSSLQSLSALLNSLETLPD
                     LPPALQKLSVGNNQLTALPELPCELQELSAFDNRLQELPPLPQNLRLLNVGENQLHRL
                     PELPQRLQSLYIPNNQLNTLPDSIMNLHIYADVNIYNNPLSTRTLQALQRLTSS"
     gene            907..993
                     /locus_tag="JNC24_001234"
                     /pseudo
     CDS             907..993
                     /locus_tag="JNC24_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1003..1083)
                     /locus_tag="JNC24_001235"
                     /pseudo
     CDS             complement(1003..1083)
                     /locus_tag="JNC24_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(1397..1873)
                     /locus_tag="JNC24_001236"
     CDS             complement(1397..1873)
                     /locus_tag="JNC24_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HAY9158038.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDIERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            complement(1932..3221)
                     /gene="bioA"
                     /locus_tag="JNC24_001237"
     CDS             complement(1932..3221)
                     /gene="bioA"
                     /locus_tag="JNC24_001237"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HAY9158039.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVVSAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKSQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKICDREGILLIADEIATGFGRTGKLFACEHAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAILESGDWQQ
                     QVADIEVQLREQLAPARDAEMVADVRVLGAIGVVETTHPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            3308..4348
                     /gene="bioB"
                     /locus_tag="JNC24_001238"
     CDS             3308..4348
                     /gene="bioB"
                     /locus_tag="JNC24_001238"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HAY9158040.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLEQALMT
                     PDTDEYYNAAAL"
     gene            4345..5499
                     /gene="bioF"
                     /locus_tag="JNC24_001239"
     CDS             4345..5499
                     /gene="bioF"
                     /locus_tag="JNC24_001239"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HAY9158041.1"
                     /translation="MSWQEKINAALDARRTADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWQQGAEKFGVGSGGSGHVSGYSVVHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFTHNDVAHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRRDEGDARREKLVSLIARFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            5486..6241
                     /gene="bioC"
                     /locus_tag="JNC24_001240"
     CDS             5486..6241
                     /gene="bioC"
                     /locus_tag="JNC24_001240"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HAY9158042.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHAELQRRSADVLLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLVQARQKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQAWQAVDERP
                     HANRFLPPDEIEQSLNGVHYQHHIQPITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            6234..6911
                     /gene="bioD"
                     /locus_tag="JNC24_001241"
     CDS             6234..6911
                     /gene="bioD"
                     /locus_tag="JNC24_001241"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HAY9158043.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAEPTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
     gene            7490..9511
                     /gene="uvrB"
                     /locus_tag="JNC24_001242"
     CDS             7490..9511
                     /gene="uvrB"
                     /locus_tag="JNC24_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HAY9158044.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVVVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(9703..10611)
                     /gene="yvcK"
                     /locus_tag="JNC24_001243"
     CDS             complement(9703..10611)
                     /gene="yvcK"
                     /locus_tag="JNC24_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HAY9158045.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     regulatory      10876..11020
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            11008..11997
                     /gene="moaA"
                     /locus_tag="JNC24_001244"
     CDS             11008..11997
                     /gene="moaA"
                     /locus_tag="JNC24_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HAY9158046.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEVGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEDDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     gene            12019..12531
                     /gene="moaB"
                     /locus_tag="JNC24_001245"
     CDS             12019..12531
                     /gene="moaB"
                     /locus_tag="JNC24_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HAY9158047.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            12534..13019
                     /gene="moaC"
                     /locus_tag="JNC24_001246"
     CDS             12534..13019
                     /gene="moaC"
                     /locus_tag="JNC24_001246"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HAY9158048.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIVGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            13012..13257
                     /gene="moaD"
                     /locus_tag="JNC24_001247"
     CDS             13012..13257
                     /gene="moaD"
                     /locus_tag="JNC24_001247"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HAY9158049.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADYPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            13259..13711
                     /gene="moaE"
                     /locus_tag="JNC24_001248"
     CDS             13259..13711
                     /gene="moaE"
                     /locus_tag="JNC24_001248"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HAY9158050.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDQQAAKRW"
     gene            13848..14552
                     /locus_tag="JNC24_001249"
     CDS             13848..14552
                     /locus_tag="JNC24_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HAY9158051.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            14757..15470
                     /locus_tag="JNC24_001250"
                     /pseudo
     CDS             14757..15470
                     /locus_tag="JNC24_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706665.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
     gene            complement(15499..16246)
                     /locus_tag="JNC24_001251"
                     /pseudo
     CDS             complement(15499..16246)
                     /locus_tag="JNC24_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005020183.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0104 family protein"
     gene            complement(16246..17487)
                     /gene="clsB"
                     /locus_tag="JNC24_001252"
     CDS             complement(16246..17487)
                     /gene="clsB"
                     /locus_tag="JNC24_001252"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HAY9158052.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DNVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGDAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRNFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLAQVDP
                     PAQPTMETQDRVETENTGVKP"
     gene            complement(17484..18245)
                     /locus_tag="JNC24_001253"
     CDS             complement(17484..18245)
                     /locus_tag="JNC24_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HAY9158053.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            18378..18788
                     /locus_tag="JNC24_001254"
     CDS             18378..18788
                     /locus_tag="JNC24_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9158054.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            complement(18750..19856)
                     /locus_tag="JNC24_001255"
     CDS             complement(18750..19856)
                     /locus_tag="JNC24_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY9158055.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(19867..21000)
                     /locus_tag="JNC24_001256"
     CDS             complement(19867..21000)
                     /locus_tag="JNC24_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY9158056.1"
                     /translation="MSNPILSWRRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQAGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            complement(20993..22729)
                     /locus_tag="JNC24_001257"
     CDS             complement(20993..22729)
                     /locus_tag="JNC24_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAY9158057.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGNATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKETTPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKHGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            complement(22722..23720)
                     /gene="hlyD"
                     /locus_tag="JNC24_001258"
     CDS             complement(22722..23720)
                     /gene="hlyD"
                     /locus_tag="JNC24_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HAY9158058.1"
                     /translation="MMKKPVVIELAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRT
                     VNLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLML
                     AGYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQA
                     TLKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTR
                     AVEPGTVLNEGGTVFTVSLTRPVWVRAYVDERYLDQAQPGRKVLLYTDGRPDKPYHGQ
                     IGFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGNEAGHE"
     gene            complement(23720..24391)
                     /gene="cecR"
                     /locus_tag="JNC24_001259"
     CDS             complement(23720..24391)
                     /gene="cecR"
                     /locus_tag="JNC24_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HAY9158059.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELLLRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGSDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            24620..25984
                     /gene="rhlE"
                     /locus_tag="JNC24_001260"
     CDS             24620..25984
                     /gene="rhlE"
                     /locus_tag="JNC24_001260"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HAY9158060.1"
                     /translation="MSFDYLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSAAIH
                     GNKSQGARTRALADFKSGDIRVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAIPGYEPDPSIKAEPIQNGRQ
                     QRGGGGRGQGGGRGQQQPRRGEGGAKSASAKPAEKPSRRLGDAKPAGEQQRRRRPRKP
                     AAAQ"
     gene            complement(26216..26698)
                     /gene="ybiA"
                     /locus_tag="JNC24_001261"
     CDS             complement(26216..26698)
                     /gene="ybiA"
                     /locus_tag="JNC24_001261"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
                     /protein_id="HAY9158061.1"
                     /translation="MPVRAQRIQHVMQDTIINFYSTSDDYGDFSNFAAWPIKVDGKTW
                     PTSEHYFQAQKFLDEKYREEIRRVSSPMVAARMGRNRSKPLRKNWESVKEQVMRKALR
                     AKFEQHAELRVLLLATAPAKLVEHTENDAYWGDGGNGKGKNRLGYLLMELREQLAIEK
                     "
     gene            26818..28968
                     /gene="dinG"
                     /locus_tag="JNC24_001262"
     CDS             26818..28968
                     /gene="dinG"
                     /locus_tag="JNC24_001262"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HAY9158062.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRVPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTANNQEEQKRCAKLK
                     GDLDTYRWDGLRDHTDIVIDDDLWRRLSTDKASCLNRNCYYYRECPFFVARREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDEVLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEAQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRVE
                     PSIDNEEQHIAEMAAFFRKQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            28996..29958
                     /gene="ybiB"
                     /locus_tag="JNC24_001263"
     CDS             28996..29958
                     /gene="ybiB"
                     /locus_tag="JNC24_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HAY9158063.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGAALRLSSVSHPEYIGRVAKFFSDI
                     GGRALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDTAGSELLPQAKDPETTAQ
                     WIERCLAGSEPIPESLKIQMVCCLVATGEAATISDGLARVNQAF"
     gene            30099..31184
                     /locus_tag="JNC24_001264"
     CDS             30099..31184
                     /locus_tag="JNC24_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate/lactate/ureidoglycolate dehydrogenase"
                     /protein_id="HAY9158064.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKTVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCAAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCNAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQKQGIPLDAGS
                     WQAICDAARQIGMPEETLQAFCQQLAS"
     gene            complement(31413..31673)
                     /locus_tag="JNC24_001265"
     CDS             complement(31413..31673)
                     /locus_tag="JNC24_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HAY9158065.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPITASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(31938..32204)
                     /gene="ybiI"
                     /locus_tag="JNC24_001266"
     CDS             complement(31938..32204)
                     /gene="ybiI"
                     /locus_tag="JNC24_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HAY9158066.1"
                     /translation="MASGWANDDAVNEQINSTIEDAIARARGEIPRGESLDECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDSQLR"
     gene            complement(32278..32955)
                     /gene="ybiX"
                     /locus_tag="JNC24_001267"
     CDS             complement(32278..32955)
                     /gene="ybiX"
                     /locus_tag="JNC24_001267"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HAY9158067.1"
                     /translation="MMYHIPGVLSPQDVARFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSTLYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSQHCV
                     PPVTRGVRVASFMWIQSMIRDDKKRAMLFELDNNIQSLKSRYGESEEILSLLNLYHNL
                     LREWSEI"
     gene            complement(32997..34981)
                     /locus_tag="JNC24_001268"
                     /pseudo
     CDS             complement(32997..34981)
                     /locus_tag="JNC24_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
BASE COUNT         8274 a         8975 c         9488 g         8244 t
ORIGIN      
        1 gaagaggtta atctttgcag ggcttgcaga gtgcgagtcg acaatggatt gttataaata
       61 ttaacatctg cataaatgtg cagattcatg atactgtctg gcaatgtgtt cagctgattg
      121 ttagggatat atagtgattg cagacgttgt ggaagttcgg gcagtctgtg tagttggttt
      181 tccccaacgt ttaaaagcct cagattttga ggaaggggcg gtagctcttg taatctgtta
      241 tcaaaagcac ttagttcctg tagttcacat ggtaattctg gtaaggcagt aagctggttg
      301 ttgccaacag aaagtttttg tagagccggg ggaagatcag gtagcgtctc caggctattg
      361 agaagggctg agagtgactg caaggaagat ggaagagaag gcaaacatgt taaaactctg
      421 ttaagtgaaa catcaagagc gaccaggtga ggcggaagag ccgggagcct gctcagtctg
      481 ttatcgctgg ctttaagtac agttaaggac ggagggagtt ctggcagaga gcgtagctca
      541 ttgctagaga tgttaagttc ttgtatgtgc gggggcaggt atgggagaga gcgtaatctg
      601 agtaaactta aattgagggc tggctcttga aaagccagac atattttcag taatcgaact
      661 gcctgtgttc ggtcttctgt tgcagcacct tccttggccc agttatccca gatgcggtga
      721 taatccgtga tattttgctc ttctacggat aaacgggata tacatcctaa accacaaaag
      781 gagcggaaaa aagaaggatt gtgggcaggt ttcatttatt attgatcctc attgtttata
      841 tattaatatg ttcgtgcaaa tggagtgagg attctttaaa aaaacgcgcc tgtaaatagt
      901 taagaacagg aagaactgtt ggggatgtgg ctggccgaaa atgaaggggt gaagttctgg
      961 ctcaatgtgc tgactgaact gaaaaaacgc ggttggtcgc cctacctttt catcaagcca
     1021 gtagtctgcc caccactgca tcatttctct gcgcttatcg agatactgag catggttgta
     1081 aattcagcta acccagtgcg ttgacaaaaa attagcgcaa gaggacaaaa aatcaccttg
     1141 cgctaatgct ctgtatcagg tcactaatac catctaagtg gttgattcat agtgactgga
     1201 tatgttgtgt tttgcagtat tatgcagtct attttttaag ctaaatgtat tataatgcat
     1261 tgatattaat gatttttact gtttctcgtt cagcttttta tactaacttg agcgaaacgg
     1321 gaaggtaaaa agacaaaaag ttgtttttaa tacctttaag tgataccaga tggcattgcg
     1381 ccatctggca gagtgattaa ctaaacatcg cagtaatcga ggcgcttgcc agagagtgga
     1441 aatgaacgtt aaacccgacc atcgcgccgc tggcaccttc atcgacatca atacgttcta
     1501 tatccagcgc gtgaacggta aaaatgtagc gatgagtttc gcctttcggc ggtgctgcgc
     1561 catcgtaccc ggttttacca aagtcggtac gcgtctgcaa aacgccgtct ggcattgcta
     1621 ccagaccaga gccaaaccct tgcggtaata cgcgggtatc agcgggtaag ttaacaacta
     1681 cccagtgcca ccagccggag ccggttggcg catccgggtc gtagcaggtg acaacaaaac
     1741 ttttcgttcc cgcaggaaca tcatcccacg ccagatgcgg tgaaatatta tcgccatcgt
     1801 aacccatgcc gttaaagaca tgacgatgcg gcaatttatc gccatcgcgc agatcgttac
     1861 tgatgagttt catgaaccct cctttcttgt ttgcagaaag tgtagccaga aaccctcacg
     1921 cggacttctc gttattggca aaaaaatgtt tcatcctgta ccgcgcggtt aaccgctgcg
     1981 gtcagacgct gcaactgttg cgggagaata atatagggcg gcatcaggta aatcagtttg
     2041 ccaaaaggcc ggatccagac accctgttcg acaaagaatt tttgcagcgc cgccatattc
     2101 accggatgag tggtttcgac cacgccaatg gcccccagta cgcgcacatc ggcaaccatt
     2161 tcggcatcac gggcgggggc aagttgctcg cgcagctgta cttcaatatc cgccacctgt
     2221 tgctgccagt cgccagattc gagaatcgcc aggctggcgt ttgctgccgc gcaggccagc
     2281 ggattgccca taaaagttgg cccatgcata aagcaaccgg cttcaccgtt actgatggtt
     2341 tctgcaacct cgcgcgtggt gagtgtggcg gaaagggtca ttgtgccgcc ggttaaggct
     2401 ttaccgaggc acaaaatgtc cggcgcgatt tctgcatgtt cacaggcaaa cagtttcccg
     2461 gtacgaccaa atccagtggc gatctcgtcg gcaatcagca agataccttc gcgatcgcat
     2521 attttgcgga ttcgttttaa ccattccgga tggtacatgc gcatcccgcc tgcgccctgg
     2581 acaatcggct caatgatcac cgccgcgatt tcatgacgat gcgccgccat caggcgggca
     2641 aagcccacca tatcgcgctc atcccattcg ccatccatac ggctttgtgg ggcgggagca
     2701 aacaagtttt ctggcagata gcctttccac aggctgtgca ttgagttgtc cggatcgcac
     2761 accgacatcg cgccaaaggt atcgccatga taaccattgc ggaaggtcag aaaacgctgg
     2821 cgcgcttcgc ctttggcttg ccagtactgc aacgccattt tcatcgccac ttccaccgct
     2881 acggaaccgg agtccgcgag aaaaacgcac tccagcggtt gcggcgtcat cgccaccagt
     2941 ttgcggcaca gctcaatggc tggcgcatgg gtgataccgc caaacatcac atgcgacatg
     3001 gcatcaattt gcgacttcat cgccgcatta agctgcgggt gattgtagcc gtggatcgcc
     3061 gcccaccagg acgacatacc gtcaaccagg cgtctgccgt cagacaaaat cagctcgcaa
     3121 ccttcggcgc tcaccaccgg ataaaccggc agcggggagg tcatggatgt gtatgggtgc
     3181 cagatatggc gttggtcaaa ggcaagatcg tccgttgtca taatcgactt gtaaaccaaa
     3241 ttgaaaagat ttaggtttac aagtctacac cgaattaaca acaaaaaaca cgttttggag
     3301 aagccccatg gctcaccgcc cacgctggac cttgtcgcaa gtcacagaat tatttgaaaa
     3361 accgttgctg gatctgctgt ttgaagcgca gcaggtccat cgccagcatt tcgatcctcg
     3421 tcaggtccag gtcagcacgt tgctgtcgat taagaccgga gcttgtccgg aagattgcaa
     3481 atactgcccg caaagctcac gctacaaaac cgggctggaa gccgagcggt tgatggaagt
     3541 tgaacaggtg ctggagtcgg cgcgcaaagc gaaagcggca ggatcgacgc gcttctgtat
     3601 gggcgcggcg tggaagaatc cccacgaacg cgatatgccg tacctggaac aaatggtgca
     3661 gggggtaaaa gcgatggggc tggaggcgtg tatgacgctg ggcacgttga gtgaatctca
     3721 ggcgcagcgc ctcgcgaacg ccgggctgga ttattacaac cacaacctgg acacctcgcc
     3781 ggagttttac ggcaatatca tcaccacccg cacttatcag gaacgcctcg atacgctgga
     3841 aaaagtgcgc gatgccggga tcaaagtctg ttctggcggc attgttggct taggcgaaac
     3901 ggtaaaagat cgcgccggat tattgctgca actggcaaac ctgccgacgc cgccggaaag
     3961 cgtaccaatc aacatgctgg tgaaggtgaa aggcacgcca ctggcggata acgatgatgt
     4021 cgatgccttt gattttattc gcactattgc ggtagcgcgg atcatgatgc cgacctctta
     4081 cgtgcgcctt tctgccggac gcgagcagat gaatgaacag actcaggcga tgtgctttat
     4141 ggctggcgca aactcgattt tctacggttg caaactgctg accacgccga atccggaaga
     4201 agataaagac ctgcaactgt tccgcaaact ggggctaaat ccgcagcaaa ctgccgtgct
     4261 ggcaggggat aacgaacaac agcaacgtct tgaacaggcg ctgatgaccc cggacaccga
     4321 cgaatattac aacgcggcag cattatgagc tggcaggaga aaatcaacgc ggcgctcgat
     4381 gcacggcgta ctgccgatgc cctgcgtcgc cgttatccgg tggcgcaagg agccggacgc
     4441 tggctggtgg cggatgatcg ccagtatctg aacttttcca gtaacgatta tttaggttta
     4501 agccatcatc cgcaaattat ccgcgcctgg cagcagggcg cagaaaagtt tggtgtaggt
     4561 agcggcggtt ccgggcatgt cagcggttat agcgtggtgc atcaggcgct ggaagaagaa
     4621 ctggccgagt ggcttggcta ttcgcgggcg ctgctgttta tctctggttt tgccgcgaat
     4681 caggcagtca ttgccgcgat gatggcgaaa gaggaccgta ttgttgccga ccggcttagc
     4741 catgcctcct tgctggaggc tgccagttta agcccgtcgc agcttcgccg ttttactcat
     4801 aacgatgtcg ctcatctggc gcgactgctt gcttccccct gtccggggca gcaactggtg
     4861 gtgacagaag gcgtgttcag catggacggc gatagcgcgc cactggcgga aatccagcag
     4921 gtaacgcaac agcacaatgg ctggttgatg gtcgatgatg cccacggcac gggcgttatc
     4981 ggggagcagg ggcgcggcag ctgctggctg caaaaggtaa aaccagaatt gctggtggtg
     5041 acttttggca aaggatttgg cgtcagtggg gctgcggtgc tttgctccag tacggtggcg
     5101 gattatctgc tgcaattcgc ccgccacctt atctacagca ccagtatgcc gcccgctcag
     5161 gcgcaggcat tacgtgcgtc gctggcggtc attcgccgtg atgaggggga tgcacggcgc
     5221 gaaaaactgg tgtcactcat tgcgcgtttt cgtgccggag tacaggatct gccgtttacg
     5281 cttgctgatt catgcagcgc catccagcca ttgatcgtcg gtgataacag ccgtgcgtta
     5341 caactggcag aaaaactgcg ccagcaaggc tgctgggtca cggcgattcg cccgccaacc
     5401 gtacccgctg gtactgcgcg actgcgctta acgctaaccg cagcgcatga aatgcaggat
     5461 atcgaccgtc tgctggaggt gctgcatggc aacggttaat aaacaagcca ttgcagcggc
     5521 atttggtcgg gcagccgcgc actatgagca acacgcagag ttacagcgcc ggagtgctga
     5581 cgtcttactg gcaatgctcc cacagcgtaa atacacccac gtactggacg cggggtgtgg
     5641 gccaggctgg atgagccgcc actggcgaga gcgtcacgca caggtgacgg cgttagatct
     5701 ctcaccgcca atgcttgtgc aagcccgcca gaaagatgct gcagaccatt atctggcggg
     5761 agatatcgaa tccctgccgt tagcgactgc gacgttcgat cttgcatgga gcaatctcgc
     5821 ggtgcagtgg tgcggtaatt tatccacggc actccgcgag ctgtatcggg tggtgcgccc
     5881 cggaggcgtg gtggcgttta ccacgctggt gcagggatcg ttacccgaac tgcatcaggc
     5941 gtggcaggcg gtggacgagc gtccgcatgc taatcgcttt ttaccgccag atgaaatcga
     6001 acagtcgctg aacggcgtgc attatcaaca tcatattcag cccatcacac tatggtttga
     6061 tgatgcgctc agtgccatgc gttcgctgaa aggcatcggt gctacgcatc ttcatgaagg
     6121 gcgcgacccg cgaatattaa cgcgttcgca attgcagcga ttgcaactgg cctggccgca
     6181 acaacagggg cgatatcctc tgacgtatca tctttttttg ggagtgattg ctcgtgagta
     6241 aacgttattt tgtcaccgga acggataccg aagtggggaa aaccgtcgcc agttgtgcac
     6301 ttttacaagc cgcgaaggca gtaggttatc ggacggcagg ttataaaccg gtcgcctctg
     6361 gcagcgaaaa gaccccggaa ggcttacgta atagtgacgc gctggcgtta cagcacaaca
     6421 gcagcctgca gctggattac gcaacagtaa atccttacac cttcgcagaa cccacttcgc
     6481 cgcacatcat cagcgcgcaa gagggcaggc cgatagaatc attggtaatg agcgccggat
     6541 tacgcgcgct tgaacaacag gctgactggg tgttagtgga aggtgctggc ggctggttta
     6601 cgccgctttc tgacactttc acttttgcag attgggtaac acaggaacaa ctgccggtga
     6661 tactggtagt tggtgtgaaa ctcggctgta ttaatcacgc gatgttgact gcgcaggcaa
     6721 tacaacacgc cggactgact ctggcgggtt gggtggcgaa cgatgttacg cctccgggaa
     6781 aacgtcacgc tgaatatatg accacgctca cccgcatgat tcccgcgccg ctgctgggag
     6841 agatcccctg gcttgcagaa aatccagaaa atgcggcaac cggaaagtac ataaaccttg
     6901 ccttgttgta gccattctgt atttggttaa attgcgagcg agatcgcgtc ttcgattgac
     6961 tgcaatttaa ccaattaaat tctaaaataa tcacgaaaaa aattttactt ctgcctcatg
     7021 cggcgaatgt gggaattgcc caggcggcgg gggatagggg ctggagacag ttatccacta
     7081 ttcctgtgga taaccatgtg tattagagtt agaaaacacg aggcaagcga gagaatacgc
     7141 ggcttgcacg cgaattggcg ttaaagacgg ctcaaagaaa tatcttttat tttttaactg
     7201 gttagataaa tgcaatggca gtcactgaac aggcatctct tgccataaaa ctgtcatcac
     7261 tcatcttgac aaatgttaaa aaagccgttg ctttggggat aacccggtaa ggccggagtt
     7321 ttatctcgcc acagagtaaa ttttgctcat gattgacagc ggagtttacg ctgtatcaga
     7381 aatattatgg tgatgaactg tttttttatc cagtataatt tgttggcata attaagtacg
     7441 acgagtaaaa ttacatacct gcccgcccaa ctccttcagg tagcgactca tgagtaaacc
     7501 gttcaaactg aattccgctt ttaaaccttc tggcgatcag ccagaggcga ttcgacgtct
     7561 cgaagagggg ctggaagatg gcctggcgca ccagacgtta cttggcgtga ctggctcagg
     7621 gaaaaccttc accattgcca atgtcattgc tgaccttcag cgcccaacca tggtacttgc
     7681 gcccaacaaa acgctggcgg cccagctgta tggcgaaatg aaagagttct tcccggaaaa
     7741 cgcggtggag tatttcgtct cctactacga ctactatcag ccggaagcct atgtaccgag
     7801 ttccgacact ttcattgaga aagatgcctc ggttaacgaa catattgagc agatgcgttt
     7861 atccgccacc aaagcgatgc tggagcggcg tgatgtggtt gtagtggcct ctgtttccgc
     7921 gatttatggt ctgggcgatc ctgatttata tctcaagatg atgctccatc tcacggtcgg
     7981 tatgattatc gatcagcgcg cgattctgcg ccgactggcg gagctgcaat acgctcgtaa
     8041 tgatcaagca ttccagcgtg gtactttccg cgttcgtggc gaggtgatag atatcttccc
     8101 ggcagaatcg gatgacattg cacttcgcgt ggaactgttt gacgaggaag tggaacgatt
     8161 gtcgttattt gacccgctga ccgggcagat tgtttccact attccacgtt ttaccatcta
     8221 cccgaaaacg cactacgtca caccgcgcga gcgcatcgta caggcgatgg aggagatcaa
     8281 agaagaactg gccgccagac gcaaagtgct gttggaaaac aacaaactgc tggaagagca
     8341 gcggctgacc cagcgtaccc agtttgatct ggagatgatg aacgagctgg gctactgttc
     8401 ggggattgaa aactactcgc gcttcctctc cggtcgtgga ccgggtgagc caccgccgac
     8461 gctgtttgat tacctgcctg ccgatgggct gctggtggtc gatgaatctc acgtcaccat
     8521 tccacaaatt ggcggcatgt atcgcggtga ccgggcgcgt aaagagacac tggtggagta
     8581 cggcttccgc ctgccatcag cgctggataa ccgtccgctt aagtttgaag agttcgaagc
     8641 attagcgccg caaaccatct atgtttcggc gacgccgggt aattacgagc tggaaaaatc
     8701 cggcggcgat gtggtggatc aggtggtgcg tccaaccgga ttgcttgacc cgattatcga
     8761 agtgcggccg gtggcgacac aggttgatga tcttctttcg gagattcgtc agcgagcggc
     8821 aattaacgaa cgcgtactgg tcaccacact gaccaagcgg atggcggaag atcttaccga
     8881 atatctcgaa gaacatggcg agcgcgtgcg ttatcttcat tcagatatcg acaccgtcga
     8941 acgtatggag attatccgcg acttgcgtct gggtgagttc gacgtgctgg tagggatcaa
     9001 cttactgcgc gaaggtctgg atatgccgga agtgtcgctg gtggcgatcc tcgacgctga
     9061 caaagaaggc ttcctgcgtt ccgaacgttc gttgatccag actattggtc gtgcggcacg
     9121 taacgttaac ggtaaagcga ttctctacgg cgataagatc accccatcaa tggcgaaagc
     9181 gattggcgaa accgaacgtc gccgtgagaa acagcagaag tacaacgagg aacacggaat
     9241 tacgccgcaa ggcttgaaca agaaagtggt cgatatcctg gcgctggggc agaacattgc
     9301 caaaaccaaa gcgaagggca gaggaaaatc gcgcccgatt gttgagccgg ataatgtgcc
     9361 gatggatatg tcgcctaaag cgttgcaaca gaaaatccat gagctggaag ggttaatgat
     9421 gcaacacgcg cagaatctgg agttcgaaga agcggcacaa attcgtgacc agttgcatca
     9481 gctgcgtgag ctgtttatcg cggcttcgtg acaggataac gaagaagaca gatgacaaaa
     9541 gcaaaattgc ctgatgtgct acgcttatca ggcctacatg atctctgcaa tacattgaat
     9601 tagcgcggtt tgtaggccgg ataaggcgtt cacgccgcat ccggcataaa caacgcgcac
     9661 tttgtcttca ataggaagcc ggaatttttc ttcggatttc cgttaaccta aagcctgtaa
     9721 cgccttttcc agcgcgttat gtaacaactg gcggtcatga cgatacggaa tatcgctggc
     9781 ctccagtacc tcctgaatca caatccgctc tttcaccgcc gagacatcca cttttggccc
     9841 gacgatgacc gcatcaatga cctttttacc aacatactgc tccataattg ccagcttgct
     9901 ttccagcttc aaattagccg caggtaaact caactcacgc cccagattgc cgatatagac
     9961 catcggcgct ggcgtgcggc gtaatgcctg ggcgatttcc ttcagcagca gaattggcat
    10021 caggctggta taaaaactgc caggcccaat aatgatgaga tccgcttcat tgatagcgtg
    10081 aaccgcctca cgcgttgcgg gtacattcgg cgttaataat aactcttgaa tcggcgtagt
    10141 taactggtcg atattgacct cgccgtaaac ttcatgcccc tgatcgtcaa tcgccatcag
    10201 atcgacggga tgctctgaca ttggaatcaa atgcgtatcc actttcagca gattacgaat
    10261 taaattgatg gcttccagag gccgcacgct aaggtgatcc agcgccttta acatcaagtt
    10321 tccgagatta tgaccggaaa gttcgccatt gccgccaaaa cggtattcaa acatcgcgga
    10381 ggcgacgctc ggttccgtta tcagttggtt gaggcagttg cgcatatcgc cccaggcaat
    10441 gccgccttct gaacggcgaa tacgccccgt cgagccacca ttatccgtgg tggtgacgat
    10501 acccgttaaa cgagaaccca aagacgaaag tgatgagaga acgcgtccca gtccatgccc
    10561 tccgccgaga gcaacgacac gatcaagatc agccagcgta cgattgcgca tatagtttcc
    10621 tggagtcaga ttatccgcgc tacagtagcg caaattgcgg ggaaacagca attaacctgc
    10681 caatattgag gatacaaact attttcttct acctctaaag gacgatgcac gctatgcctc
    10741 cctgatgatg tatatcaaag taaaactgcc attttccctt attctgtagc gaaatagcac
    10801 gatcatgacg ctatatacat gattacatag cgaaagtgtg gatggtaaaa atctcattta
    10861 cacgctagta tcggcttaac cactaaacac tctagcctct gcacctgggt caactgatac
    10921 ggtgctttgg ccgtgacaat gctcgtaaag attgccacca gggcgaagga agaaatgact
    10981 tcgcctcccg tatctggaaa ggtgtacatg gcttcacaac tgactgatgc atttgcgcgt
    11041 aagttttact acttgcgcct gtcgattacc gatgtgtgta actttcgttg cacctactgc
    11101 ctgccggatg gctacaaacc gagcggcgtc accaataaag gctttcttac cgtcgatgaa
    11161 attcgccggg ttacgcgcgc cttcgccagt ctgggcaccg aaaaagtgcg cctgacagga
    11221 ggagagccgt ctttacgccg cgactttacc gatatcatcg ccgctgtgcg ggaaaacgac
    11281 gctatccgcc agattgcggt caccaccaat ggttaccgtc tggaacgcga tgtggcgaac
    11341 tggcgcgatg cgggacttac tggcattaac gtcagtgtcg acagtctgga cgcccgccag
    11401 tttcacgcta ttaccgggca ggataaattc aaccaggtca tggcagggat cgatgctgca
    11461 tttgaggtcg gttttgagaa ggtcaaagtc aataccgtgc tgatgcgtga tgttaatcat
    11521 caccagctcg acacctttct gaactggatc cagcatcgcc ctatccagct gcgtttcatc
    11581 gaactgatgg aaacgggcga gggcagtgag ctcttccgta agcatcacat ctctggtcag
    11641 gttctgcgtg acgagctact gcgtcgcggc tggatccacc aattacgtca acgcagcgac
    11701 ggtcctgcgc aagtcttttg ccatccagat tacgccggag agattggcct tatcatgccg
    11761 tatgaaaaag acttctgcgc cacttgcaac cgcctgcgcg tttcctccat tggtaaactc
    11821 catctctgcc tgtttggtga aggcggcgtt aacctgcgcg atctgctgga agacgatacc
    11881 cagcaacagg cgctggaagc gcgtatttca gcggcgctgc gggagaagaa acagacccat
    11941 ttcctgcatc aaaacaacac cggtattacg caaaacttat cgtacattgg cggctaaaac
    12001 gtcaaaagga gagatcagat gagtcaggta agcactgaat ttatcccgac ccgtattgct
    12061 attcttacgg tttctaatcg tcgcggtgaa gaagacgata cctccggtca ctatttgcgc
    12121 gattcggcgc aagaagcggg ccatcacgtt gtcgataaag ccattgtgaa agaaaaccgc
    12181 tacgctattc gcgctcaggt atctgcgtgg atcgccagcg acgatgtaca agtggtgttg
    12241 attacggggg ggactggcct gacggaaggt gatcaggctc ccgaagcatt gctgccgttg
    12301 ttcgaccgtg aagttgaagg ttttggtgaa gtgttccgga tgttgtcgtt tgaagagatt
    12361 ggcacttcca cgttgcaatc tcgtgcggta gcgggcgtcg ccaacaaaac gctgattttc
    12421 gccatgccgg gttcgaccaa agcgtgccgt accgcatggg aaaatatcat cgcgccgcag
    12481 ctggatgccc gtacgcgtcc gtgtaatttc catccacatt tgaagaaata agtatgtcgc
    12541 aactgaccca tatcaacgcc gctggcgaag cgcacatggt ggatgtctcc gccaaagcgg
    12601 aaaccgtgcg tgaagcgcgg gcggaagcct ttgtcaccat gcgcagcgag acgctggcga
    12661 tgattattga tggtcgccac cacaaaggcg acgtatttgc cactgcgcgt attgtcggta
    12721 ttcaggcggc aaaacgcacc tgggatctga tcccgctgtg tcacccgctg atgctcagca
    12781 aagttgaagt caatttacag gccgagccgg agcacaatcg ggtgcgtata gaaaccttat
    12841 gccgcctgac cgggaaaacc ggtgtcgaaa tggaagcgtt aaccgcggcc tccgtggcgg
    12901 cgctgaccat ttatgacatg tgcaaagcgg tgcaaaaaga tatggtgatt ggtccggtac
    12961 gtttgctggc gaagagcggc ggcaagtcgg gtgactttaa ggtggaagcg gatgattaaa
    13021 gttctttttt tcgcccaggt gcgcgagttg gtgggaacag atgcaaccga agtggctgcg
    13081 gattacccaa ccgttgaagc gttacgccag cacctggctg cgcagagcga tcgctgggcg
    13141 ctggcgctgg aagatggcaa attgctggct gccgtcaacc agacgctggt gagttttgac
    13201 catccgctga ctgacggcga cgaagtagct ttcttcccgc cggtaaccgg aggttaagat
    13261 ggcagaaacc aaaattgttg tcggcccgca gccgttcagc gtaggagaag agtacccgtg
    13321 gctggctgag cgtgacgaag acggtgcagt ggtcacgttt accggtaagg tgcgcaacca
    13381 taacctgggt gacagcgtca aagcattaac cctcgaacac tatccgggga tgactgaaaa
    13441 agcactggca gaaatcgttg atgaagcgcg taaccgctgg ccgctggggc gcgtcacggt
    13501 gattcaccgc atcggggaat tatggccggg cgatgaaatc gtttttgtcg gtgtcaccag
    13561 tgcgcatcgc agcagtgcgt ttgaagccgg gcagtttatt atggattatc tcaaaacccg
    13621 cgcaccgttc tggaagcgcg aagccacgcc ggaaggcgac cgctgggttg aagcccggga
    13681 gagcgatcag caggcggcaa aacgctggta gttttttgtt agccggataa ggcaccgctg
    13741 cgtccggcaa tttcaccgct cagtacatgc gtttacgctg cgtcgatgca cagcctcatc
    13801 attttgcagt atccttaaga tattccttat atcttcagga gatcgtcatg gacagattcc
    13861 cacgttctga ttcaatcgta caaccccggg ctggcttgca aacttatatg gcgcaagtct
    13921 atggctggat gaccgttggc ttgttgctga ccgcatttgt tgcctggtat gcggctaatt
    13981 ccgcggccgt gatggagctg ttgttcacta accgtgtctt tttaatcggt ctgatcatcg
    14041 cgcaattagc attggttatt gtgttatcag cgatgattca aaagctgagc gcaggtgtaa
    14101 cgacgatgct ctttatgctt tattcggcgc tgacgggtct tacgctttcc agtatattca
    14161 ttgtctatac cgctgcttct atcgccagta ctttcgtcgt tactgccggg atgttcggcg
    14221 caatgagcct gtacggttac accacgaagc gcgatttaag tggctttggc aatatgctgt
    14281 ttatggcgtt aatcggcatt gtgctggcat cgctggtcaa cttctggctg aaaagcgaag
    14341 cattgatgtg ggcagttacc tacatcggcg tgattgtctt tgtcggattg acggcgtatg
    14401 acactcagaa attgaaaaat atgggtgagc agattgatac ccgcgacacg tcgaacctgc
    14461 gcaaatattc cattcttggc gcgttaacct tgtatctgga cttcatcaac ctgttcctga
    14521 tgttgttacg gatcttcggc aaccgccgtt aattcttttt caccatcact atcccggcca
    14581 tgtgtcggga tttttttctt actaaaaacg acacttaaca agtaccaggt ataaaatatg
    14641 ccttatgagc gagcggaatc gttttccgta tccataaata caaaagtatt ttgtatgcgt
    14701 ggttttaaat ttattgatgg tgaatattaa tattttttct aaggacaact aaaagaatgg
    14761 aatcatactc gcaaaacagc aataaattag attttcagca cgaggccagg atataaaacg
    14821 gtatatggct cattaccgct ttaggtttgg tggcaaccgc aggactagcc tggggagcta
    14881 agtatctcga aattacggca accaaatatg attcaccacc aatgtatgtc gctatagggt
    14941 tattattact ttgtatgtat ggcttaagta aggatatcaa caagataaat gccgccatcg
    15001 cgggcgtaat atatctgttt ttactctctt tggtggcgat tgtcgttgca agtttagttc
    15061 ctgtatatgc cattatcatc gtgttcagca ctgcgggcgc gatgttttta atcagtatgc
    15121 tggccggttt attatttaat gttgagcctg gttctcaccg ttttatcatt atgatgacgt
    15181 tgacagggtt ggccctggta atcatcgtga atgcggcatt aatgagtgaa cggcccattt
    15241 gggtaataag ttgcttaatg attgtgttat ggccaggcat tatctcgcat ggacgaaata
    15301 agctccttga actggcagga aaatgccata gtgaagagtt gtggagtccg gttcgttgcg
    15361 cttttacagg tgcattaaca ctctattact attttatcgg cttctttggg atacttgccg
    15421 cgatagctat aacgcttgtc tggcaaaggc atacgcgttt ttttcattag ccgtaaatgc
    15481 acatactaaa gaacttaact aaacttcaca tcgccgcttc atttttcgcc cgtagcccag
    15541 tacaaagaaa tagttcacgc tttgaccaag taacagccag ataatcgccc ccattaccat
    15601 ccagttaacg ctggaaatca gcatctgagc gagggcgaat ttccatgaag gcagcaccag
    15661 cttttgccct ttgatggtca tatggcggtg cttcgcgaaa gcgcaaaacc acaaataaac
    15721 cgcgataatc atcagtaagc caatgccgag aatacgcagc gtagtttgat cgacatacca
    15781 gtgatccggc aactccacca cgcctgcggt aaagataatc cctgccagta aaatatagcc
    15841 cagccagttg gtggtaatac tgagcgagaa aatccgcgta atagtgctac cgggcaaccc
    15901 cagacgagag tacaaacgat agcgcatacc aatgccgccg acccaggtac tgagcgtcag
    15961 gttgaaggcg tagcagataa acgacaccag catcacctgg cgcttcgcca gtttgtgacc
    16021 gcagtaaaag cgggcgagca ggtcatagca gccgtaaatc agatagctga cgaccaccag
    16081 cgaccgcact aagcagcgca acgcgattgt agtcgcggat gaccttccag acctcttccc
    16141 agtccacttt tttggcgtag accaccagca acacaatcac cgcgataaaa aacagccagg
    16201 tgaggatctt ctttgctaag cgccagcgcg ggtgtgattt actcatcagg gttttacccc
    16261 cgtgttttca gtttctaccc gatcctgcgt ttccattgtc ggttgtgcgg gcggatcaac
    16321 ctgcgccaga cgtggcgtgt gtgccggaag ccagccaacc agcgccggga agtggcgtaa
    16381 aaagtggaac gccagcacgc ttttggtcag gttccaccag gtgcgtttgg gcagcatggt
    16441 ttcatccacc tgctgacaat ctgcggcgat aatgccgttc agattatcgc gcagcgtctg
    16501 gttaaaatta cgatcgtgga tgatgacatt tgcttcgaga ttcagtgaca aactgagcgg
    16561 atcgagatta ctggacccta ctgtcgccca gtgatcgtcc atcaatgcta ctttgccgtg
    16621 gagcgggcgg cggcggtact caaacacctg aacgccgcct ttaaccagat agttatacag
    16681 caagcgcgcg ccgactctga caatcggcat atccggttcg ccctgaatga tcagtttgat
    16741 ccgcaccccg cggcgtgccg ctttacgcaa ggcgtgtaaa aatcgatagc cggggaagaa
    16801 gtaggcgttg gcgataatga cttcccgccg cgcctgagtg agcattttca gatagtggcg
    16861 ttctatatca tcgcggtgtt cttcgttatc gcgccagacc agcaatacct gcgcgtctcc
    16921 cggctggcgg ttctcttccg ctttgtgatg acgtcgccac cagcgtcgtg ccgcgctctg
    16981 tccaggcagg ttttccagct caaactggag aatatcttcg acaatcggcc cttcaaggcg
    17041 taccgcgtaa tcctgtttag cctctggacc gtagctggac atatgctcgg cggagtaatt
    17101 cagcccgcca ataaaggcga tacgcgcgtc gatcaccaca attttgcgat gcatccggcg
    17161 aaacacattg gtgcgcatac caaaaaggcg agggcgggga tcgtagtagc ggaacactac
    17221 gccagctgcc gtcagttcat tgacaaactc atcgctgaga tccggcgaac cgtagccatc
    17281 cagcaagact tccgctttaa ccccgcgttg cgctgctgcc agtagtgccg catgcagctg
    17341 tttgccgacg ttatcctcaa accagataaa cgtttcaaga atgatgcgtt cttgtgcctc
    17401 gccaatcgcc ttaaacaccg cgggataata ttgctcgccg ttttccagca actggatctt
    17461 attgccttcg cgccagctac atttcataaa tgaatctccg cacttaaagg ggcatgatca
    17521 gaaaggtgtc gccatgtccg cagcggcaac gcggttggcg cgctggcgct ggcatttttg
    17581 acgtagatcc tgtccagtcg tagtagagga aattgcaccg gaaacgtgcg cgccgggcgt
    17641 ccgtgggcgc gggtaaaaat ctcatccagt ccggcctgca cttttaacgg atgattagct
    17701 ttttgccgcc agtcattgaa atcacccgcc accaataccg gttcgccgtc cggtagctca
    17761 ttcacccatt cggcgagcat cgcaagctgc gcctgacggt gcgcctcacg caggcccaga
    17821 tgtacgcaca tcacatgaat cgcttttccg gtcatcggcg gcacaatgcg gcagtagagc
    17881 acgccgcgct tttccgcacc atcgaccgaa acatcgcgat tctcataatg ttcaatggga
    17941 taacgcgaca gtacggcgtt gccgtgatgc ccttccgggt atacggcatt gcgaccgtag
    18001 gcaaaatcgc tccacatagt gtcggcgaga aactcgtagt gcgaggtatc gggccagttt
    18061 tccacatgca gcggatgaac ttcgtgcgcg cccatcactt cctgcaggca aacaatatcg
    18121 gcgctgacgg tacgcacggc atcgcgaagt tccggcaaaa tgaagcgtcg gttaaacgcg
    18181 gtaaagcctt tgtgaatgtt gatggtgagc accttgaacg aaaattgttg tgtttgatcg
    18241 ggcataattt tcctgtcttt gcctcttatc tcattgaaat agtgtagtcg gcgtcacaaa
    18301 aaggtgcggt cttacggaat tttccgtaaa gttcggtact ctgagtaagt agagataaat
    18361 tcttcaggag agaagccatg aagtggcaac aacgtgttcg tgtcgcaacg ggtctaagtt
    18421 gctggcagat tatgttgcat ttactggtag tggcgctgct ggtggtgggc tggatgagta
    18481 agactctggt tcacgtcggc gtgggattat gcgcactgta ttgtgtcacg gtagtgatga
    18541 tgctggtgtt tcagcgccac cccgagcaac gctggcgtga ggtggcagac gtgctggaag
    18601 agctgaccac gacctggtat tttggcgcag cgctgattgt gctgtggctg ttgtcccgcg
    18661 tactggaaaa caactttttg ctggcaattg cagggctggc gatccttgct ggcccggcgg
    18721 tagtgtcttt gctggcgaaa gataagaagt tacatcacct tacgtctaaa catcgcgtac
    18781 gccgctgacc ctgtcgtggc cgttatcacc agtagcggcc acaaactatt ccacacaata
    18841 tccagactcg catccttcaa ataaatctgc ttggtaatgt ccgtaaagtg gcgaataggg
    18901 ttaatccacg tcaggttttg cagccatact ggcatgtttt ccaccggaga aacgtaaccg
    18961 gaaaggagaa tggcgggcat cataaaaaca aacacgccga taaacgcctg ctgttgtgtc
    19021 gagcagagtg atgaaatcaa cagaccgaat cccaccagcg ataaaccgta aatcaccatc
    19081 gtaaagtaga acagcgccag cgatccggcg aaggggattt gatacgccca gataccaatc
    19141 gccagcacaa tggtggcctg gaaggtggcg acaattaacg ccggtacggc tttgccgatg
    19201 aagatctgcc aggtggtgag cggcgaaacc agtagctgat cgagcgtacc ttgttcacgt
    19261 tcgcgggcga cggaaagtga agtgacgatc attacgccga tagtggtgat catggcgatc
    19321 agcgacggca ccacaaacca tttgtagtca agattcgggt tataccagtt gcgtaccacc
    19381 agctcgctgt tgttaggttt cggttttcct tccagcagct cctgctgata atttttgacg
    19441 atctgttgca ggtagttggc ggcaatttgc gcactgttgg agttacgccc gtcgaggatc
    19501 aactgcaacg gcgcggtctg gaaggtatcc agtttgcgcg agaagtcagc cgggaaacgc
    19561 accagtagta acgccttttg tgtgtcgatg gttgggcgga tctcctgtgg gcttttcagc
    19621 agcagcacat gggtaaaggc gctggcgcgg gcaaaacgtt gggtcagctc caccgaatgc
    19681 tcgccgttat cttcatcgta gatggcgatg gtggcgttag tcacttccag cgtggcggcg
    19741 aacgggaaca ggatcacctg aataagcaca ggtaaaatca gaatcgcgcg ggtttgtggt
    19801 tcgcgcagca acgactgcaa ctctttgcgg attaacgtcc ataagcgatg aaacatgctc
    19861 ttctccctaa tccagccgac gtttggtttt cagccacgtc aggccgataa acatcaccgc
    19921 cgaagcgatc aaaaacagca cgtttaccac cagcaccact ggaatattcc cggcgaggaa
    19981 caggctttgc agggtgctga cgaaataacg agcgggaata atgtacgtca ccgcgcggat
    20041 caccgcgggc atactgtcga tctgaaaaat aaagccggaa agcataatcg acggcaggaa
    20101 cgcggcgttc agggcgacct gagcggcatt gaactggttg cgggtaatcg tggaaatcag
    20161 cagccccatc cccagggtac tgagtaaaaa caggctggag ataaaaaaca gaataagcag
    20221 cgaaccgcga tacggcacgc cgagaataaa cactgacacc agcatacaca gcagcatcgc
    20281 cagcatcccg agaaagtaat aagggatcag cttacacagc agcagttccg tgcgggtaat
    20341 ctccgtagag agcagagcct ccatggtgcc gcgttcccat tctcgcgcca ccaccagcga
    20401 ggtgagaatc gcgccgatga ccgtcatgat gatggtcact gcaccgggga taatgaagtg
    20461 ctggctaatc gccgccgggt taaaccagta gcgggtttgt acatcaataa gcggttcaaa
    20521 agtctgcccg ttgtcctccg ctcgctgcat ttgccagatc tgccagatcc cttcgacata
    20581 cccctgtaca aagttagcgg tattcggctc actgccgtcg gtgatcacct gaatcggtgc
    20641 ggtggcgttg gcgcgctcca tctgttccgc aaaatccacc ggaataacca ccagaccgcg
    20701 aattttcccc gcctgcattt tggcgatcag ttcctgacgg ttatcgctga tggtggcgtc
    20761 gatgtagggc gaaccggtca tggtgtgggt gaaatccagc gccgcttcgc tacgctgttc
    20821 cagcaagatc ccgacccgca gcttgctgga gtcgaggtta atgccataac caaaaataaa
    20881 cagcagtagc agtgggatca ctaccgcaat cagccagcta ctcggatcgc gaacgatctg
    20941 ccgcgtctct ttgacgcaca gcgcccgcac gcgacgccag gaaaggatcg ggttactcat
    21001 tgctatgctc cttatcccag tcgtggatca actgaataaa ggcttgctcc atggtgggat
    21061 cgggttgctc atcgttagcc gactgtgctt tcaaatcgtc cggcgtaccg ctggcgatta
    21121 atttcccacg gtacaccagg ccgatgcggt cgcaatattc cgcttcatcc ataaagtggg
    21181 tggtgaccat caccgtgacg cctttctcta ccatgctgtt gatatgtagc caaaattcac
    21241 ggcgggtgag gggatcaacg ccggacgtcg gttcgtcgag aaacagaatg tccggttcat
    21301 gcatcagcga acaggccagc gccagccgct gtttaaaacc taatggcagt tcatcggtgg
    21361 cgtgggaggc gatacttttc aggccgaacg cctcgctcat gcgggagatt ttttcgttct
    21421 gcgcccgacc gcgtaagcca tacacaccag agaaaaagcg taaattctgt tcgaccgtca
    21481 ggttaccgta gagcgaaaac ttttgcgcca tatagccgag atgctggcgc gctttaccag
    21541 aactctcttt cagatccatc cccagtacca gcgcctgacc agaggtcggc accagcaaac
    21601 cgcacatcat cttaaaggtg gtcgatttgc ccgcgccgtt tggccccagc aaaccaaaaa
    21661 tctccccgtg tttaacggca aagttgacgt gatcggtggc ggcaaaatca ccaaatttct
    21721 tggtcagttc tttcgcttcg atcaccgtct cgccgggtgt gccttctacc gtatgtaata
    21781 ttgcgcccag cggcgattcc gaggttccgg caccgcccag caaatcaata aacgcatctt
    21841 caaaacgcgg cgtagtttcg ttgatgttga tttccggcat cccgtcggca tggcgaatat
    21901 cgtctggtgt ggtctctttt ttgaggatca gacgtaccga tttcccctga atcatgccgt
    21961 cgctgacctg cggcagtttc aatgcccgtt gcaacagttt gcggttgccc tcgtgtggac
    22021 ttgtcatcag aaagctgcgt ccggccatgg tttgtgtcag ggcttttggt tccccctgat
    22081 acagcaactc gccttcattc atcagtaaca cgtcacggca ctgctcggct tcgtcgagat
    22141 acgaggtact ccagaggatt aacatccctt cgcctgccag ctcatgcacc atctgccaca
    22201 gttcgcgccg tgagataggg tcaacgccga cgccgggttc atcgagcagc aacactttcg
    22261 gttcgcccac cagggtacag gccaggccga gtttttgttt catcccaccg gagagcttgc
    22321 ccgccaggcg tccggtaaac ggcccaagag acgtaaactc cagcaggcga gcaaaagttt
    22381 gcttacgtgc ctcgccggtg acgctgcgca aatccgcgta cagattgagg ttctccatca
    22441 ccgtgagatc ttcatacaga ccaaatttct gcggcatata gccgagcacg gcgtgcagcg
    22501 cgccgtcgtt tttgattgga tcaaagccaa tcaccgtggc attgccgctg tcgggtttca
    22561 gtaatcccgc cagcatccgc attagcgtgg ttttacctgc accatccggc cccaccagcc
    22621 ccgtcacata accggcgtga atggtacaat cgagcggcgc gacggcgggc ttgtccatgc
    22681 caggaaagcg tttttccagg ccgttcagcg tgataacggc atcattcatg tcctgcctcg
    22741 ttaccgaatt gcaccgtcac cggcattccc tggcgtaacg catcatcggc gtcggtcacc
    22801 acaatacgca ggcgatagac gaggtcggta cgcagatccg gtgtttcgac ggttttcggg
    22861 gtaaattcag cagtcggcga aacgaaacca atctgcccgt gatacggctt gtccgggcga
    22921 ccatcggtat aaagcagcac tttgcgcccc ggctgggcct ggtcaagata ccgttcatca
    22981 acataagcgc gcacccacac cggacgcgtt agtgaaacgg taaacaccgt gccaccttca
    23041 ttgaggaccg tgcctggctc caccgcgcgc gttaacagcg tgccatcaga cggggctatc
    23101 aacgttgagt cctgtaaatt caactccgcc tgcgccagtt gcgcctgcgc ctgttcgagg
    23161 ctggctttcg cctgggcgat gtcctgttca cggttaccgg aacggtactg acgcaattta
    23221 tcctgtgctg atttcagcgt tgcctgcgcc tggtcgcgcg aggagcgggc attttccagg
    23281 tcatttgccg aaatagtgcg gcttttccac agtccttgct ggcggttata gaagttctgc
    23341 gcatagtcat aggcggcttg cgcctgtttc accgctgcgg cggcctgagc gatttcttca
    23401 tcgcgatacc cggcaagcat cagatcatac tgcgcctgtg ccaccgagac gcccgctttc
    23461 gcctgcatca gggcaatctc atacggcttg tgatccagtt cgcccagcac ctggcccgct
    23521 ttgatagcat caccttcgtc caccgccagc gattcaacgc gccccccaac acggaaacta
    23581 agattcaccg tacgaatatc cacgttgcca tacagcgtca ggccgttatc ctggcggctt
    23641 tgataccacc agtagcctcc ggcaaccacg gcggcaagta ccactaccgc cagttcgatc
    23701 acgacaggtt ttttcatcac tacaaactcc tttgcgataa tccttgcaaa atcaggtcga
    23761 tatgacaggt caccgtctgg ttgatcagtt cggtcttttc ttcatcgaac gcggtccagc
    23821 cggtacgtaa cagaattgtt tctttgccaa gacggaacgc cagaatctca ccaatcaacg
    23881 catgggtatg aaggatcatg cgggtgtcat tggcgtcgct gccggtccag gcggcaatca
    23941 ggcgtgtcag gtggctgtgt agcggactaa tcacctgttc gtgcaccagg tggtaggctg
    24001 ccgtgggaga gagctgctca cgggagataa acttgctgag gttgacggtg tcatcctggg
    24061 tgagcagctt aatcatgttc ctgcaggcgc gaaggagcag ttcacggatg gcagcccgat
    24121 caggctgtgg ttgggcgaac aagcgttcgg cttcctcggc atgcggacgg aactgctcgc
    24181 caataaaatc ggcaatccac tgggcgcagg cgaggtacaa atcttctttc gaaccgaagt
    24241 agtaggtgat ggcagcgata ttctgcccgg cttgggcggc tatctcgcgg gtggtggcgt
    24301 tcattccata ttcaccaaac tgcgccagtg cggcagcaat cagctgtttt ttcgcctgtt
    24361 cacccttgat tgtcatggca ggattattca tcgcacagtc cattcttaat caaatgattg
    24421 attaagatta tgactccata tgggagttgt ccagtatggc taagaatttt agctacgcca
    24481 gtcacaggga taatttatgc gctgcgtcac aaaaactgct acactccgct cgctcatgac
    24541 attgtggttt ttgtcatttt ccttttcagt atctccctga aaactacacc ggtaacggtc
    24601 ggggcggttc ggagtagtca tgtctttcga ttatttgggt ttaagccctg atatcctgcg
    24661 cgccgttgcc gagcagggtt accgtgaacc cacccctatt cagcagcagg cgatccctgc
    24721 ggtgctggaa ggccgcgacc tgatggctag cgcccagacc ggcaccggca aaacagcggg
    24781 ctttacgctg ccgctgttgc aacacctgat cactcgccag ccgcacgcca aagggcgtcg
    24841 tccggtacgt gcgctcattc ttaccccgac ccgtgaactg gcggcgcaga ttggcgaaaa
    24901 cgtccgtgat tacagcaaat acctgaacat tcgttcgctg gtggtgtttg gtggtgtgag
    24961 tattaacccg cagatgatga aactgcgtgg cggcgttgat gtgctggtgg caacgccggg
    25021 acgtttgctg gacctggaac atcagaatgc agtgaagctg gatcaggttg aaatcctcgt
    25081 cctcgatgaa gctgaccgca tgctcgacat gggctttatc cacgatatcc gtcgcgtgtt
    25141 aacaaaacta cctgcgaagc gccagaacct gttattctcc gcgaccttct ctgacgatat
    25201 taaagccctg gcggaaaaac tgttgcacaa cccgctggaa atcgaagtgg cacgccgcaa
    25261 taccgcgtct gatcaggtga cccagcacgt tcactttgtc gataagaaac gcaaacgcga
    25321 attgctgtcg cacatgattg ggaaagggaa ctggcagcag gtgctggtgt ttacccgtac
    25381 caaacacggc gctaaccatc tggctgaaca gctcaataaa gatggcatcc gtagtgcggc
    25441 gatccacggc aataaatcgc aaggtgcgcg tactcgtgcg ctggctgatt ttaaatcggg
    25501 cgatattcgt gtactggtgg caactgacat cgctgcgcgc ggcctggata ttgaagagct
    25561 gccgcacgtc gtcaactatg aactgccaaa cgtaccggaa gattatgtcc atcgtatcgg
    25621 gcgtaccggt cgtgcggctg ctaccggtga agcgttgtcg ctggtgtgtg ttgatgaaca
    25681 caaactgctg cgtgatatcg aaaaactgct gaaaaaagag atcccgcgca ttgcgattcc
    25741 gggctatgag ccggacccgt caatcaaagc cgaaccgatc cagaacggtc gccagcaacg
    25801 tggcggcggc ggtcgtgggc aaggtggtgg tcgcggtcaa cagcaaccac gccgtgggga
    25861 aggtggcgca aaatctgcaa gcgcgaaacc tgcagaaaaa ccgtctcgcc gcctcggcga
    25921 tgccaaaccg gcaggcgaac aacaacgtcg ccgccgtccg cgtaaacctg ccgctgcgca
    25981 gtaatctttt atgccgggct atgcccggca tcaggctgat gaacaaacgc aaaactgcct
    26041 gatgcgctac gcttatcagg cctacgtgaa ctctgcaata tattgaattt gcatgctttt
    26101 gtaggccgga taaggcgttt acgccgcatc cggcatttca caacaagcac ttgtcagcaa
    26161 tttgagaaca cgggaaaaaa tttatctgtt ttaccgccca taagccaccg agacgttact
    26221 tctctatagc caattgttct cgcaactcca ttaaaaggta gcccagtcga ttcttgccct
    26281 taccattacc accgtctccc cagtaagcat cgttttccgt atgctcaacc agttttgcgg
    26341 gcgcggttgc cagcaagagc acacgcagtt ctgcatgctg ttcgaattta gcacgaagtg
    26401 ctttacgcat cacctgttct ttaaccgact cccagttttt acgaagtggc ttggaacgat
    26461 tacgccccat gcgagcggcg accatggggg aagaaacccg acggatctct tcgcggtatt
    26521 tttcatcaag gaatttttgt gcctgaaaat agtgttcgga ggtgggccag gttttcccgt
    26581 caaccttgat gggccaggcg gcaaagttag aaaaatcacc gtagtcatcg ctggtgctgt
    26641 agaaatttat gatggtgtct tgcatgacgt gttggatcct ttgtgctcga acgggcatta
    26701 aaccgcatta tgttggtggt tattgcgagc cgctttccag aaacagaaaa accattaccc
    26761 ctgaaaaccg aaaaatgcca caatattggc tgtttataca gtatttcagg ttttctcatg
    26821 gcattaaccg ccgcgcttaa agcgcaaatt gccgcctggt ataaggcgct tcaggaacag
    26881 atccccgact ttattccccg tgtgccgcag cggcagatga ttgcggacgt cgccaaaacg
    26941 ctggccggag aagaaggccg gcatctggcg attgaagccc ccaccggcgt tgggaaaacg
    27001 ctctcctatt tgatccccgg catcgccatt gcccgcgaag agcaaaaaac gctggtggtg
    27061 agtaccgcca acgtggcgtt gcaggatcag atttacagca aagatttacc gctgctgaaa
    27121 aagatcattc ccgatcttaa attcactgcc gctttcgggc gtgggcgcta cgtttgtccg
    27181 cgtaatctga cggcgctcgc cagcactgaa cccacgcaac aggatctgct ggcgtttctt
    27241 gacgacgaac tgacggcaaa taatcaggaa gagcaaaaac gttgtgcgaa gctgaagggc
    27301 gatctcgaca cttatagatg ggatggtctg cgcgatcata cggatatcgt tatagatgat
    27361 gatctctggc gtcgtttgag taccgacaaa gccagctgcc tcaaccgtaa ctgttactac
    27421 tatcgtgaat gcccgttttt tgttgctcgt cgggagattc aggaagcgga agtggtggtg
    27481 gcaaaccatg cgctggtgat ggcggcgatg gaaagcgaag ccgtactgcc tgacccgaaa
    27541 aatctgctgc tggtgctgga cgaaggtcat cacctgccag atgtggcccg ggatgcgctg
    27601 gagatgagcg ctgaaatcac cgcgccctgg tatcgactac agctggactt gttcacgaaa
    27661 ctggtcgcta cctgcatgga gcagtttcgc ccgaagacca tcccaccgct ggcgatccct
    27721 gaacgtttga atgcgcattg tgaagagttg tatgagctta tcgcctcgtt aaacaacatt
    27781 ctcaatctct acatgcctgc cgggcaggag gcagagcacc gttttgcgat gggcgaactg
    27841 cctgatgaag tgctggagat ctgccagcgg ctggcaaaac tcaccgagat gctgcgtggt
    27901 ctggcggagt tatttcttaa cgatttaagt gagaaaaccg gcagccatga cattgtacgc
    27961 ctgcatcggt tgattttgca gatgaaccgc gcgttgggga tgttcgaggc gcaaagcaaa
    28021 ctctggcggc tggcgtctct ggcgcaatct tccggtgcgc cggtgaccaa atgggcgacg
    28081 cgggaagagc gcgaagggca actgcatctc tggtttcact gcgtggggat acgtgtcagc
    28141 gatcagctgg aaaggctgct gtggcgcagt attccgcaca ttattgtcac ctccgcaacc
    28201 ttgcgttcgc tgaatagttt ttcgcgtttg caggagatga gtggtctgaa agagaaagcg
    28261 ggcgaccgtt ttgtggcgct ggattccccc tttaaccact gcgaacaggg caaaattgtt
    28321 attccccgga tgcgcgttga gccttccatc gacaacgaag agcagcatat tgccgaaatg
    28381 gcggcctttt tccgtaagca ggtggagagc aaaaaacatc tcggtatgtt ggtactgttt
    28441 gccagcggac gggcgatgca gcgctttctc gactatgtga cggatttacg tctgatgttg
    28501 ctggttcagg gcgatcagcc gcgttaccgt ttagttgaac tgcaccgcaa acgcgtcgcc
    28561 aacggtgagc gcagcgtgct ggtgggctta cagtcatttg ccgaagggct tgatttgaaa
    28621 ggtgatctgc tcagccaggt gcatatccac aaaatcgctt ttccgcccat cgacagcccc
    28681 gtggtgatca ccgaagggga atggctgaaa agcctcaacc gctatccgtt tgaggtgcaa
    28741 agcctgccga gcgcctcgtt taacctgatt cagcaggttg ggcgactgat tcgaagccac
    28801 ggttgctggg gcgaagtggt tatctacgac aaacgcttgc tgaccaaaaa ttatggcaag
    28861 cgactactgg atgcattacc ggtatttccg atagagcaac cggaagtccc tgaaggtata
    28921 gttaaaaaga aagaaaaaac gaaatcccca cgccgtcggc ggcgttaatg atgtgagtca
    28981 ggtaaggagt cgtaaatgga ctatcgcaaa atcattaaag agatcgggcg cgggaaaaac
    29041 cacgcgcgcg atttagaccg ggatactgcg cgcggtctgt atgctcatat gctcaatggt
    29101 gaagtccctg accttgagtt gggcggcgta ttgattgcgc tgcgtatcaa aggagaaggg
    29161 gaagcagaga tgctcggctt ttacgaagcc atgcaaaatc acaccatcaa gctgacgccg
    29221 ccagcaggca agccgatgcc gattgtcatc cccagttaca acggcgcgcg taaacaggcc
    29281 aacctgacgc cgttgttggc gattctcctg cacaaactcg gttttcctgt ggtggttcac
    29341 ggggttagcg aagatccaac ccgcgtgctg actgaaacca tttttgaatt gatgggtatt
    29401 acgccaacgt tgcacggcgg gcaggcgcag gcgaagctcg acgaacatca accggtgttt
    29461 atgccagtcg gcgcgttttg cccgccgctg gaaaaacaac tggcgatgcg ctggcggatg
    29521 ggcgtgcgta acagtgcaca taccctggcg aaactggcga cgccatttgc cgaaggtgcg
    29581 gcgctgcgtc tttccagcgt ttcgcatccg gaatatattg gacgcgtcgc gaagttcttt
    29641 agcgatatcg gcgggcgggc gctactaatg catggtactg aaggtgaagt gtatgctaat
    29701 ccgcagcgct gcccgcagat caatctcatt gaccgtgaag ggatgcgggt gctgtatgaa
    29761 aaacaggaca ctgctggtag cgagttactg ccacaagcaa aagatccgga aaccacggcg
    29821 cagtggattg agcgttgcct tgctggcagc gaaccgattc ccgaatcgct gaaaatccag
    29881 atggtttgct gcctggtggc tacgggtgaa gcggcaacta tcagcgacgg cctggcgcgc
    29941 gttaatcagg cattttaatt cttttctcag ccggatgagc catgtcatcc ggcttttacc
    30001 ccaccgcaat atgaaattcc tgcatcttta ttgaccttcc cacgcccggc gtgcagcata
    30061 aaaatacaac aaacacataa cataaacagg agttaaccat ggaaagtggt catcgctttg
    30121 atgctcagac gctgcacagt tttattcagg ctgtatttcg tcagatgggt agcgaggaac
    30181 aagaagcgaa attagttgcc gatcatttaa tcgcggcaaa cctggcaggg catgattcac
    30241 atggtattgg catgatccca agctatgtac gctcctggag tcaggggcac ctgcaaatta
    30301 accatcatgc caaaaccgtt aaagaggcgg gggcggcggt cacgctcgat ggcgatcgcg
    30361 catttggtca ggtcgcggca catgaagcga tggcgctggg gattgagaaa gcgcatcagc
    30421 acggtattgc cgccgtggcg ctacataact cgcatcatat cggccgtatc ggttactggg
    30481 cggagcagtg tgcagcggcg gggtttgtct ctatccactt tgttagcgtg gtcggtattc
    30541 caatggtcgc gccgttccac ggtcgcgaca gccgctttgg caccaatccg ttctgtgtgg
    30601 ttttccctcg taaagataat ttcccgctgt tgcttgatta cgccaccagc gccattgcat
    30661 ttggcaaaac ccgcgtcgcc tggcataaag gcgtccccgt gccgccaggt tgcctgattg
    30721 acgttaacgg cgtgccgacg accaatccgg cggtaatgca ggagtcgccg ttgggttcgc
    30781 tgttgacctt tgccgaacat aaaggctacg cccttgcagc gatgtgtgaa attcttggcg
    30841 gggcgctttc cggcggtaaa acgacgcatc aggaaacgtt acaaaccagt cccgatgcca
    30901 ttcttaactg catgaccact atcatcatca acccggaact cttcggcgcg ccggattgta
    30961 acgcgcagac cgaagccttt gccgagtggg tgaaagcctc gccacatgat gatgataagc
    31021 cgattttgct accgggcgag tgggaagtga acacgcgtcg cgaacggcag aagcagggga
    31081 ttccactgga tgcgggaagc tggcaggcca tttgtgatgc agcgcggcag attggtatgc
    31141 cggaagagac gttgcaggct ttctgtcagc agttagccag ctaaaaaaaa gcccgtccag
    31201 tggcggacgg gcaaacaagg gtaacatagg atcaatgagg gttagagcat atgcgtctgt
    31261 cggcaaacag acagggaaat acttgtgctg gacgtagcgt aaacgcctga tccgtccaac
    31321 ggttcggatt ttgtaggcct gataagacgc ggtagcgtcg catcaggcat ctgttgccgg
    31381 atgcggcgta aacgccttat ccgtcctacg aattacttat aaattaccgc agtaccgctt
    31441 aatttattgt tgttggtggc ggaagtgatg ctgtatccac tggcaccggc tgccgcggct
    31501 ttctcagcca gtttcgcttc cagggcatcg agggtggatg cgccatcggc agaaaccacg
    31561 ccgattttat tcatgttctg tgcctgggat gccgttatcg gttccgccgc gaacacgcca
    31621 aatgacagag ttgaaagagc catagcagca acaacagtat tgatagtttt cataattaat
    31681 ctctcgcagg tgatttttgt ataagtgacg ttgtttcgtc gatgtgatga gtatcacgtt
    31741 tttttacgag agataaaatc gaagagaatt gaccgccttg ttcaaataaa ttgattgata
    31801 tctaacatac tgataaacca tttatttgtt gtacttatga acttgtttta taccgcgtgg
    31861 cagtcacagc agcgttaaag gcacggaatg ccacgcaaaa taaaaattat actcgctatg
    31921 catctcgcca gttgtagtta acgtaactgg ctgtctttcg aacctctgcg attatatcct
    31981 gtataagctg gtttttgtaa atctttctcc tgctgacaat gaatacataa gcgcacgcca
    32041 ggaatggctt cccgacgggc ctgcgggatg ggggcaccgc actcttcaca ttcatccagg
    32101 ctttcgccgc gcggaatttc accccgagcg cgggcaatcg catcttcaat tgtactgttg
    32161 atctgttcgt tgacggcgtc atcgttagcc caaccggatg ccatatcgac ctccccatat
    32221 caatacttgt acagttaagt gtagctaatc cagggacgaa ctcgggcagt tcaagcatca
    32281 gatctccgac cattcccgca gcagattatg ataaagatta agcagcgaca ggatctcttc
    32341 actttcgccg tagcggcttt tcagcgactg aatattgttg tccagttcaa acagcatggc
    32401 gcgcttttta tcatcgcgga tcatcgactg gatccacata aatgatgcca ctcgtacgcc
    32461 gcgggttacg ggtggcacgc aatgctggct gctggaggga tacaacacga gatcgcctgc
    32521 cgggagtttt acccgatgtt gtccgaaggt gtcattaacg accagttcgc cgccgtcgta
    32581 gctttgtgga tcgcttaaaa acagcgtggc agaaaggtca gtacgcatcc agccgttttg
    32641 tggatggctg cgtactgcgc catccacatg gaaaccatag gtttcattgt tctgatagcg
    32701 attaaacagc ggcgtggaaa gggtacgcgg caaggccgcg gcaaagaaca aagcatgttg
    32761 gttaaccgcg ttcagcacct cattttgcag ggcggcgtat aacgtgctgc gggtgtcgac
    32821 ctgttgattg ttcttaactt gcgcaccttg tgcgccggtg gtgacgcgtc catccaccca
    32881 ttcggcttgt tccagttgtt cgcgaaaccg ggcgacgtcc tgtggcgata acacgccggg
    32941 aatgtggtac atcatacaag ttctccaaaa agtggggcct gcgccccaca tctgaatcag
    33001 aaatgcatat tggctgtgag caagaaggtt cttggctcgc ccgggtgata acggtagccg
    33061 ctcttgttga ttgaggcgac gtaatcagta tcaaacaggt tgtagacgtt tagctggaag
    33121 tcgagattgc gattaacccg ataccccagt ttggcatcgg cgacccagta accttcggta
    33181 aatgctggcg ttcccaccgc gccgtctgaa cctttatgca tactgccgat atagcgtgcg
    33241 cccgcgccaa cagagatatc gtcggttgcc tgatattggc tccataaggt gaaggcgtgc
    33301 tccggggtat acggcagcga tgaggaacca tcctgggcaa catctttgcc gtttttgatg
    33361 gttgcttttt gctgggtata gccgccaatc acctgccacg cgggagtgat attcccggcc
    33421 acggatatct catagccttc gacgcgtttc ttaccgtatt gcgagtaagt tccgtcatca
    33481 ttttgctcaa cttcattttc gatatcagta cggaacagcg cggcggtgag caacagacgt
    33541 ttatccagaa cctgccattt ggtgccaatc tcgctggtgt tggctttttg cggtttaaaa
    33601 tcggtgcggt tggcactgtt accgctgcca gactgcgcaa gggcgaagtt gttgccgccc
    33661 ggaggctgct gggaaacggc atagttaata tagacattgc cgttttccgt caggtgatac
    33721 agcgccccgg ctttccagtt catcagattg cccgacttgg cggtgtcgac ggtggtgacc
    33781 ggagaacctt ttgccacacc agttgggcag gtgatggcac cgcgtccgct gccgccgcag
    33841 gcggtggcac tgtcatattc agtatgataa ttatccagac ggatcccgcc gttcagctca
    33901 aaatcacggg tgatttgcag cgtatcaaag gcgtaaattg cgaaggtatc cgtctgacca
    33961 ttggcgtttg cgccgttgcg cgtcaggccg ccgggatgaa tgctgctgtc aggatgataa
    34021 atatttaccg cgggtaacgt caccggatta acgccgtagt tagtctgcgt ttcacgggta
    34081 aattccacgc cggtactgac atcatgaccg atagaaccgg tatagaacgt cgaggtcagg
    34141 ttggtctggt tggtgagaat tttattactc acatctttgg tattcgccgt gcgtgaccag
    34201 gttcagctat tcacatcgct ggtgggctga gtaatattcg acgccccgcc cataatcgcc
    34261 gtcatcaggt aatcctgctt tacgcgcgac caacgggtag tattgcgaat ggtggtgtta
    34321 tcgttgatgt cgtgctcaaa acgcatggtg gcggtgtcgg tggtcgaatc gtcgtaatcg
    34381 gaatccgtgc cgtaaaagtt atgagtatca acttttccgg aatgattcag ggccgccgtt
    34441 cccgcagatg gggcagaata gcccggcaaa ccgatggtcg gaatgccgcc gtctggcgtg
    34501 ttgtgctggg tgacatgcag ataattaaga tacaaacgat tcgctgtacc aaggccaaaa
    34561 gcgacagaag gggcgacgcc gtaacgctca tttttgactt tgtcgcgtcc ggcatcgtgc
    34621 gttttttcgc ccattacatt caggcgcacc gcggtggtat caccaatgac ctgattgacg
    34681 tccagcgtgc cgcggcggaa ccaggcgctg ccaatactgg cggaggcgtc aatgccggaa
    34741 tcattgcgcg gctgtttgct gatcatattg atcgagcccg tcggtgcgct gcgcccgtag
    34801 tcggtgccgg acggcccttt aatcacttcg acctgctcgg tattgaaggt gtcgcgcgag
    34861 acgctgccga tatcgcgaat gccatcaata taaatactgt tagaggtatc ggcaccacgc
    34921 atataaatgg cgtcgccagt ggtggagtta ccgttctcac ccgcaaaaaa cgcacccacg
    34981 c
//
LOCUS       DADMKF010000029        34001 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.029, whole genome shotgun sequence.
ACCESSION   DADMKF010000029 DADMKF010000000
VERSION     DADMKF010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34001
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.029"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            12..514
                     /locus_tag="JNC24_001269"
                     /pseudo
     CDS             12..514
                     /locus_tag="JNC24_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311186.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
     gene            complement(581..2038)
                     /gene="nuoN"
                     /locus_tag="JNC24_001270"
     CDS             complement(581..2038)
                     /gene="nuoN"
                     /locus_tag="JNC24_001270"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HAY9158068.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGLMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPMGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLYSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSASGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(2045..3574)
                     /gene="nuoM"
                     /locus_tag="JNC24_001271"
     CDS             complement(2045..3574)
                     /gene="nuoM"
                     /locus_tag="JNC24_001271"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HAY9158069.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRLGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     AEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     EIASQELPGMSLRELFMILLLVVLLVLQGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(3738..5579)
                     /gene="nuoL"
                     /locus_tag="JNC24_001272"
     CDS             complement(3738..5579)
                     /gene="nuoL"
                     /locus_tag="JNC24_001272"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HAY9158070.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEILHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAIVGILLA
                     AWLWLGKRTLVTSIANSAPGRLLSTWWYNAWGFDWLYDKVFVRPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(5576..5878)
                     /gene="nuoK"
                     /locus_tag="JNC24_001273"
     CDS             complement(5576..5878)
                     /gene="nuoK"
                     /locus_tag="JNC24_001273"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HAY9158071.1"
                     /translation="MIPLQHGLILAAILFILGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(5875..6429)
                     /gene="nuoJ"
                     /locus_tag="JNC24_001274"
     CDS             complement(5875..6429)
                     /gene="nuoJ"
                     /locus_tag="JNC24_001274"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HAY9158072.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(6441..6983)
                     /gene="nuoI"
                     /locus_tag="JNC24_001275"
     CDS             complement(6441..6983)
                     /gene="nuoI"
                     /locus_tag="JNC24_001275"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HAY9158073.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPIYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(6998..7975)
                     /gene="nuoH"
                     /locus_tag="JNC24_001276"
     CDS             complement(6998..7975)
                     /gene="nuoH"
                     /locus_tag="JNC24_001276"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HAY9158074.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(7972..10698)
                     /gene="nuoG"
                     /locus_tag="JNC24_001277"
     CDS             complement(7972..10698)
                     /gene="nuoG"
                     /locus_tag="JNC24_001277"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HAY9158075.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQSKIDVIVQALAGAKKPLIISGTNAGSIE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRHASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     HVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(10751..12088)
                     /gene="nuoF"
                     /locus_tag="JNC24_001278"
     CDS             complement(10751..12088)
                     /gene="nuoF"
                     /locus_tag="JNC24_001278"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HAY9158076.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(12085..12585)
                     /gene="nuoE"
                     /locus_tag="JNC24_001279"
     CDS             complement(12085..12585)
                     /gene="nuoE"
                     /locus_tag="JNC24_001279"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HAY9158077.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(12588..14390)
                     /gene="nuoC"
                     /locus_tag="JNC24_001280"
     CDS             complement(12588..14390)
                     /gene="nuoC"
                     /locus_tag="JNC24_001280"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HAY9158078.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(14484..15146)
                     /locus_tag="JNC24_001281"
     CDS             complement(14484..15146)
                     /locus_tag="JNC24_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017490251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B"
                     /protein_id="HAY9158079.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(15162..15605)
                     /gene="nuoA"
                     /locus_tag="JNC24_001282"
     CDS             complement(15162..15605)
                     /gene="nuoA"
                     /locus_tag="JNC24_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HAY9158080.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(16234..17172)
                     /gene="lrhA"
                     /locus_tag="JNC24_001283"
     CDS             complement(16234..17172)
                     /gene="lrhA"
                     /locus_tag="JNC24_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HAY9158081.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPIEMMSPYLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
     gene            18087..19304
                     /locus_tag="JNC24_001284"
     CDS             18087..19304
                     /locus_tag="JNC24_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAY9158082.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            19388..19987
                     /gene="yfbR"
                     /locus_tag="JNC24_001285"
     CDS             19388..19987
                     /gene="yfbR"
                     /locus_tag="JNC24_001285"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HAY9158083.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNSQIAQEYKAIEKIAQQ
                     KLVNMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(20046..21878)
                     /locus_tag="JNC24_001286"
     CDS             complement(20046..21878)
                     /locus_tag="JNC24_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HAY9158084.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTGVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTDEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(21965..22613)
                     /gene="hxpA"
                     /locus_tag="JNC24_001287"
                     /pseudo
     CDS             complement(21965..22613)
                     /gene="hxpA"
                     /locus_tag="JNC24_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416796.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HpxA"
     gene            complement(22624..23118)
                     /locus_tag="JNC24_001288"
     CDS             complement(22624..23118)
                     /locus_tag="JNC24_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HAY9158085.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(23201..23656)
                     /locus_tag="JNC24_001289"
     CDS             complement(23201..23656)
                     /locus_tag="JNC24_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF412 domain-containing protein"
                     /protein_id="HAY9158086.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGQLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            23994..25196
                     /gene="ackA"
                     /locus_tag="JNC24_001290"
     CDS             23994..25196
                     /gene="ackA"
                     /locus_tag="JNC24_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HAY9158087.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            25271..27415
                     /gene="pta"
                     /locus_tag="JNC24_001291"
     CDS             25271..27415
                     /gene="pta"
                     /locus_tag="JNC24_001291"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HAY9158088.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            27605..29125
                     /gene="yfcC"
                     /locus_tag="JNC24_001292"
     CDS             27605..29125
                     /gene="yfcC"
                     /locus_tag="JNC24_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAY9158089.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATMIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGTRMMIAPALLVGFAKGILLLVGNGEAGDASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(29158..29700)
                     /gene="yfcD"
                     /locus_tag="JNC24_001293"
     CDS             complement(29158..29700)
                     /gene="yfcD"
                     /locus_tag="JNC24_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HAY9158090.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(29758..30309)
                     /gene="yfcE"
                     /locus_tag="JNC24_001294"
     CDS             complement(29758..30309)
                     /gene="yfcE"
                     /locus_tag="JNC24_001294"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HAY9158091.1"
                     /translation="MKLMFASDIHGSLPATERVLELFAQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMLLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(30365..31009)
                     /gene="yfcF"
                     /locus_tag="JNC24_001295"
     CDS             complement(30365..31009)
                     /gene="yfcF"
                     /locus_tag="JNC24_001295"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HAY9158092.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLKPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RRIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLALGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            31145..31792
                     /gene="yfcG"
                     /locus_tag="JNC24_001296"
     CDS             31145..31792
                     /gene="yfcG"
                     /locus_tag="JNC24_001296"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HAY9158093.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLTEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRQRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            31849..32211
                     /gene="folX"
                     /locus_tag="JNC24_001297"
     CDS             31849..32211
                     /gene="folX"
                     /locus_tag="JNC24_001297"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HAY9158094.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            32232..33125
                     /locus_tag="JNC24_001298"
     CDS             32232..33125
                     /locus_tag="JNC24_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family protein"
                     /protein_id="HAY9158095.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHEQKERLCQSRWNITQKLVDLINAS
                     DTPPSVFISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNDLRG
                     PFNMVSPYPVRNEQFAHALGQALHRPAILRVPATAIRLLMGESSILVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(33173..>34001)
                     /locus_tag="JNC24_001299"
     CDS             complement(33173..>34001)
                     /locus_tag="JNC24_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HAY9158096.1"
                     /translation="DTARDFIDIHLPAPLRKLCDLTTLKLESNSFIDEDLRQYYSDLL
                     WSVKTQEGVGYIYVVIEHQSKLEELMAFRMMRYSIAAMQNHLDAGYKELPLVIPMLFY
                     HGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDITVVPDDEIMQHRKMALLELIQK
                     HIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQTGDAQRFRAFIGEITERAPQE
                     KEKLMTIADRLREEGAMQGKHEEALRIAQEMLDRGLDRELVMMVTRLSPDDLIAQSH"
BASE COUNT         8479 a         9280 c         8564 g         7678 t
ORIGIN      
        1 aggaaaaata aatgggaatg attggttatt ttgcagaaat tgactcagaa aagattaacc
       61 aactgcttga gtctactgaa aaacctttga tggataatat acatgacacg ctttcaggat
      121 tacgccgact ggatattgat aaacgctggg attttttaca ctttgggctg acaggcaccc
      181 ctgcttttga tcccgcgaag aatgatccgc tgagtcgtgc cgtgcttggt gaacacagcc
      241 tcgaagatgg catagatggc ttcctgggat tgacctggaa tcaggagtta gccgcgacaa
      301 tcgaccgact ggaatcactg gatcgaagtg agctgcgtaa acagttttcc attaagcgac
      361 tcaatgaaat ggagatttat ccgggagtga ccttttcaga agagttagag gggcaactct
      421 ttgcttcgat catgctgata tggaaaagct gattagcgcg tatcgacgta tgctgcgaca
      481 agggaatcat gcgctgacgg ttattgttgg gtgatggtgt cagggcacca aaaaaaaccg
      541 ccgaacatgt cggcggcttt ctgacttaca aactgatagc ttacatcagc ggcattgcta
      601 accgcacaat gctaatcagc ggttgtggcc atacacccag caccagtacc aacagtgcag
      661 agatcagcac cacgataccg cctgcgctgt actgccagtt tgacggtgca tcgcgacccg
      721 gttgttccgg ggcgtgcagg tacaggctca ccgccacgcg caggtagtag taaaggccgc
      781 ttgccgaacc gacaaccacg gcgcccacca gccaccacaa gtgtgcctgg acaccgaccg
      841 ccagcacgta gaacttacca ataaagccca gcgtcatcgg aataccggcc agagacagca
      901 tcatcaccgt catcaccgcc gcgaggatcg ggcgatgcca gaacagtccg cggtaagagt
      961 acagggaatc agcatccggg ccacgatacg ggctggacat caggctgacc acgccgaacg
     1021 cgccgaggct gctaaacaga taaccggcca ggtaaacccc taccgcttcc atcgacatct
     1081 cgccagtttg cagcgcaatc agcgccacca gcagatagcc gaggtgagag atagatgagt
     1141 aaccgagcag acgtttgata ttggtctggc tcagcgccat caggttaccg aagatgatgg
     1201 aggcaaaggc gataatcgcc agcaccacgc gaatcgcttc gctgtcaccc atcggtgcgt
     1261 agaggaacag acgcatcacc acaccgaaga tagcgatttt gctcgccgtc gccaagaaag
     1321 tggaaaccgg cgcaggcgcg ccctggtata cgtctggcgt ccacaggtgg aacggcacca
     1381 gagagagttt gaagccgagg ccaacaatca tcaggccgaa acctgccagc aacagcggct
     1441 cgttgagcat accgtcgcca aggtttttac ccaacgcgac aaacgacagg tcgccagact
     1501 gcgcatacac cagcgccata ccaaacagca ggaaagaaga cgctgcggca gaaaggatgg
     1561 tgtatttgat actggcttcc agtgaacgtt tctggcggaa agcgtaaccg accaggccaa
     1621 acagcggcaa agagatcagt tcgataccga ggaacagaga cgccagatgg ttggcattcg
     1681 ccagcaggat cccgcccagc gcggcaatta acaccaacag gtagaactcg tccttgttgt
     1741 cgttatagcc ttcaagccac gggtaggcga aagtacaggt ggcgaggctc gccaacaata
     1801 ccagcccggt gtaaagcatg gcgaaaccat caacgcgcat cagcggcgta acgtccatag
     1861 cgcctgcctg gccgacaaac cagagcgaaa ccagcgccgc gttaagccca ataaccgaga
     1921 gcgtagcgtt gaggaaatga ttgcgtcgcc acgcaatgga gagcatcaca accaccaccg
     1981 tcaagccgac gatcagcaac ggtagcagtg cgatcaggtt ttgtggagtt attgtcatgg
     2041 cgatttacgg ccttgtagta gtaacggaat taacaaacca ctgctggata ttgccaatcg
     2101 cggagtgcga ggtatccaga atcggctgcg gatagaagcc ctgcagtacc agcagcacca
     2161 ccagcaacag gatcataaac agctcacgca gcgacatccc tggcagttcc tggctggcaa
     2221 tctcgctttt cgctttaccg aagtaagcgc gatgcaacat cgccagcgaa taaacagatg
     2281 caaagaccag cccaaaggta gagataacgg taatcaccgg gacaacctgg aagctgccga
     2341 acagaatcat aaattcgccg acgaagttac cggtgccagg catcccaagc gttgccaccg
     2401 caaagaacag cgacagtgct ggcagccatt tcatcttgct ccacagaccg cccatcatgc
     2461 gcatgtcgcg ggtatggata cgttcataaa gctgaccaca aagaataaac agacccgccg
     2521 ccgacaagcc gtgcgcaatc atctggatta ccgcgccctg gtaggccaac tggctgccgg
     2581 tgtagatagc aatcagcacg aagcccatgt gggaaaccga ggtgtaggcg atcagacgtt
     2641 tgatatcggt ctgggcgaag gccatccacg caccgtagaa gatgccgata acacccagcc
     2701 acatagcaat tggcgcgaac tctgccgacg cgttcgggaa cagcggcagg gagaaacgca
     2761 gcaaaccgta agcggcagtt ttcagcaaga tccccgcgag gtcaacggaa ccggcggtcg
     2821 gagcctggga gtgcgcatcc ggcagccagc catgcagcgg aaccaccggc attttgactg
     2881 cgaaggcgat gaagaagccc agcattaaca ggtattccgc accactggac attggcgtat
     2941 tcagcagctc ttcatagttg aaggtccaga cgccggtcgc attgtagtga acaaaaacca
     3001 gcgccaggat ggcaatcaac atcaccagac cactcgcctg ggtgtaaatg aagaacttgg
     3061 ttgccgccgt gatacgcgtt ttaccgtcag aggctttatg cccccacagt gcgatcagga
     3121 agtacatcgg caccagcatc atttcccaga agaagaagaa caggaacatg tcgatggcaa
     3181 ggaacacgcc gataacgccg cccaggatcc acatcaggtt gaggtggaag aagccctgat
     3241 atttttcgat ctctttccac gaacatagta ccgccagcac accgagcaga ccggtcagca
     3301 cgaccatcag cagcgacagc ccgtcaatgg cgagatgaat agagataccc aaacgcggga
     3361 tccacggcat gtcgaattca gactgccact gcggaattcc ggcggattgc gtcagtgaat
     3421 aaccgccctg caaccacagt tgcagcgaca gcgccagcgt caatcccatg gtgatcagcg
     3481 cgatccagcg tggcaccttg acgccaaagc gttcggtctg ccagcacagg aagccgccaa
     3541 taaagggaat taatattagc cagggtagta acatggcgat ctttattcct tgtaaaagtc
     3601 ccgtcaggac cggattttca acgaattctc acgacagaaa attctcttcg gactgggggt
     3661 agtgtcggat gcaccgcgaa ggcggcttat ccgacctacg ttcttatatg cccaggggca
     3721 atcccacaat ccttaactca acgcagtacc atcaacagtg ccagcaccac gaccgcaccg
     3781 atgctcatgg atgccacata ccagcgcaga tagccgttct cacttaacag cagaccttta
     3841 cctgcaaagc gggaaaggac agccggaatg ttcatcattg agttcagcgg atcgcgtttc
     3901 agcaaccagg caatacccag gaacggcctg acgaacactt tgtcgtacag ccagtcaaag
     3961 ccccaggcgt tgtaccacca ggtactcagc aggcggcccg gcgcactgtt ggcgatggag
     4021 gtcaccagag tacgtttacc cagccacagc caagctgcca gcagaatgcc gacaatcgcg
     4081 accacgccag aggtaatttc cagggtcaac atgctgccgt gcgccagttc cgtcgtttgc
     4141 ggaagcacgc cctgcagcgg cggtacaatc agtgcgccaa cgaaggtgga aaggatcagc
     4201 agcacaatca gcggcaggct gtgagttacc cctttcacgg cgtgagcgtg aatttgttct
     4261 tttccgtgga agacgatgaa aatcatacgg aaggtgtaga gcgaggtcat aaacgcaccg
     4321 accagacctg ccaccatcag attgatatga ccattcgcca tcgcacccgc gaggatctca
     4381 tccttactga agaagcccgc agtgaccagc ggcagtgccg acagtgctgc gccgcccacc
     4441 aggaagcaga gataaaccag cggaatagat ttacgcagac cgcccatctt gaagatgttc
     4501 tgttcgtgat ggcaggccag aatgacggaa ccggatgcca ggaacagcag cgctttaaag
     4561 aacgcgtgag tcatcaagtg gaaaatcgcc gcatcccatg cctgcacgcc aagcgcgagg
     4621 aacatgtagc caatctggct catggtagag taagcgagaa cacgtttgat gtcggtctgt
     4681 accagcgcgg caaaaccggc cagcagcagc gtaaccgccc cgacaatgcc caccagatgc
     4741 agaatttccg gcgtcatcag gaacaggccg tgggtacggg cgatcaggta gacacccgcg
     4801 gttaccatgg ttgcggcgtg gatcagcgcg gagacaggcg tcgggcccgc catcgcgtcg
     4861 gcaagccatg tctgcaacgg caactgcgca gatttaccga ccgcaccgcc cagcagcatc
     4921 agcgtcgccc acatcagcat gttattgccg tcagcaaagt gcgctggtgc cagttccacc
     4981 atttcgcgga agttcagggt gcccagttcg ttgtaaagaa tgaacagtgc gaaagcgagg
     5041 aaaacgtcac ccacacgggt cacgacgaac gctttcattg ccgctgcgcc attcttcgga
     5101 tcggtgtaat agaaaccgat cagcagatag gagcacaggc ccacgccttc ccagccgagg
     5161 tacatcagca gcaggttgtc ggcaagcacc agaactacca tgctggcgat gaacaggttg
     5221 gtgtaggcga agaagcgaga gtagccctct tcaccgcgca tgtaccagga ggcgtacatg
     5281 tgaataagga aacccacacc agtgaccacc gagagcatgg tcagcgacag gccgtccagc
     5341 accaggttaa aaccgatgtt aaagtcgcct accgacatcc acgtccacag cggctggctg
     5401 tatgcctgct cgccgttagc gaagaaatca acgccgataa aggcggttac cagcgccgcc
     5461 aggcccacag agccaacgcc gacgatcgcc gaaacgtttt cagaccagcg cccacgggag
     5521 aacgccagca ggacgaagcc aatcaatggc aaaataatgg ttaaggcaag catgttcatc
     5581 cgcgcatctc acttactgaa tcgatgttca ggttctggcg acgacggtga agttgcagca
     5641 gcagcgcaag gccgatactc gcttctgccg ccgcgaggct gatggcgaga atgtacatca
     5701 cctgaccgtc ggtctgaccc cagtagcttc cggcgacaac aaaggccagc gcggaggcgt
     5761 taatcatgat ttccagacca atcagcataa acagcagatt gcgacggata accagaccgg
     5821 ttaagccaag aatgaataag attgccgcga ggatcagtcc atgttgtaag gggatcatgc
     5881 gtgctcctcc gtttttcttt tcgcgctgtc gtctttacga ttgctcagca cttcacccgc
     5941 acgctcttca cgaccgacgt ggaaggccac aaccagacct gcgagcagca gcatagaagc
     6001 cagttccacc gccagtacgt aaggcccgaa cagcgtaata cccactgctt tagcactgat
     6061 tggcgtaccg tcgataccct gatcgttaac accgaggatg gcgtaaacaa tcaccaccag
     6121 catgatggcc gacaaaattg ccggaccaat ccacacctgc ggtttcagcc actggcgttc
     6181 ctgttcgatt tctgaaccgc ccaggttgag catcatcacc acgaacacga acagcaccat
     6241 aatggcaccc gcgtagacga taatttccag cgcaccggcg aagtaagcgc ccagtgagaa
     6301 gaacaccccg gaaatcgcca gcagcgaaat aatcaggtac agcagtgcgt gtaccggatt
     6361 ggtatgggtg atcactcgca aggtcgcaag tatggctatc aggccacaga tataaaaagc
     6421 gaactccatt gcccctctcc ttacggtaac aggctcttga cgtcgatagg cttggcttcg
     6481 ttctctgctt cgcccttatc tttgccgtcg attgccatac ctgccatccg gtagaagtta
     6541 tattccgggt atttgcccgg accggagatc agcagatcct ctttttcgta aaccagatcc
     6601 tggcgcttgt attcccccat ttcgaaatcc ggggttaact gaatcgccgt ggtcggacag
     6661 gcttcttcgc acagaccaca gaaaatgcag cgtgagaagt tgatgcggaa aaattccgga
     6721 taccagcgac cgtctttggt ttctgctttt tgcagcgaga tacagccgac cgggcaggct
     6781 accgcgcaga ggttacaggc tacgcaacgc tcttcgccgt ccgggtcgcg ggtcagaacg
     6841 atacgaccac gataacgggg cggcagatag atcggctctt ccgggtacat tcgcgtttcg
     6901 cgtttggcga acgcgtgcag gccgatcatc cagatactac gaacctgggt gccgaaacct
     6961 actaacaatt cttttaaggt catggtctta ttgcccctta ttgcgcctgc cagagaatga
     7021 cagccgccgt taccagcaag ttgatcagcg tcagcggcag gcagattttc cagccgaagg
     7081 acattacctg gtcataacgc ggacgcggta acgacgcacg aatcaaaatg aacatcatca
     7141 taaagaacgc ggttttcagc gcgaaccaga tgaatggcgg taacaacggg ccttgccagc
     7201 caccgaagaa cagcgtcacc atcaatgcag agatggtcac aatcccgatg tactcaccca
     7261 cgaagaacag accgaacttc ataccggaat attcaatgtg gtaaccatcc gccagttcct
     7321 gctcggcttc cggctggtca aacgggtgac ggtgacatac cgccacgccc gcgatggcaa
     7381 aggtaataaa accaaagaat tgcgggataa cgttccacac atgcgcctgg ctgttgacga
     7441 tgtcggtcat gttgaatgaa ccggcctgcg ccaccacgcc catcagggaa agcccgagga
     7501 acacttcgta gctcagggtc tgcgcagaag cacgcatcgc acccagcaac gagtatttgt
     7561 tgttacttga ccagcccgca aacagcaccg cgtaaaccgc cagacctgcc atcatcagga
     7621 agaacaaaat cccgatgttc aggtcggcaa ccacccaacc cggactgact ggcacaatcg
     7681 caaaggccag cagcagcgag gtaaaggcaa tcatcggtgc cagggtaaag atgacgcgat
     7741 ccgagaattt cgggatccag tcttctttaa agaacatttt gatcatgtcc gcaaccagct
     7801 ggagcgaacc gccccagcca acacggttag gtccgtaacg gttctggaac agacccagca
     7861 ggcgacgttc gccaaagctc atgaatgccc cgcaggtgac aaccaccagc aggatcacca
     7921 ccgctttgag gatggtcagc aggatctcaa tcagttccgg tgatatccaa ctcattgttg
     7981 tgcctccttg agatcctcaa gatgcgcgcc agccagcacc ggagcaatac cggacatacc
     8041 catcggcaag cccacctgcc ctgccgtcag cccttcagcg atttcaaccg gcagcgtgac
     8101 cgtattgcca tcgtaactaa aggagacgtg tgtacctgcg ttcacaccta acttcgcggc
     8161 atccgctggg ttgagtttga tgtacggctg cggcatacgg ctctggaaga ccggagcacg
     8221 ctgtgacaat tcatcgctgc caaacaggtg gtaatacggc gcgatacgcc atttcccgtc
     8281 ctgcggctgg aagcgtgccg gtacgctggt gaagtaatcc agaccatttt cgctggtttc
     8341 aaacagacgc acgcccggat cgccaaagcg cagtttgccg cccacttcgt cctggaattt
     8401 gttccacgcc tgcggggagt tccagcccgg cgcccaggca aacggcactt gcgaacggtg
     8461 cgcagtcggc tggttgttac cttccatcga gaaggtgaac atggtgtcaa tatcctgcgg
     8521 ctgacgcggc tcatgaacgc tgatattggc gcgcatggcg gtacgaccgc tgtaacggtg
     8581 cggttcacgg gccagtttct gcccacgaat acggaatgtc gcatccggcg cagcatcttt
     8641 gatacctgcc agttccggga ttttcgccac aacagcgtca atcacatggt cgagctgcgt
     8701 ccagtccact tcacggctca gcagggtgct gtgcagcgag tgtaaccagc gccagctttc
     8761 cagcatgaca gttttgctgt cgtaataagc aggatcgtaa acctggaaga aacgttgggc
     8821 gcggccttcg ttgttgatca ccgtaccgtc gctttcagca aaactggcag cagaaagtac
     8881 cagatgggcg ttttccataa tcgctgtgcg ttgatgatca accaccatca ccagcggcgc
     8941 tttagccagc gcagcattca cgcgggtagc ggaagcgtga cgatgcagat cgttttccag
     9001 caccaccacc gcgtcggcgc gtccggtttc cagttcggtc aacgcttctt caagcgaacc
     9061 gccacccata atgcccagcc ccatgctgtt gacggaacgg gcaatcatgg tgataccaac
     9121 gtcagcgccg cgacctttca gggctttcgc gacgttagcc gcagcctgaa tcacctctat
     9181 gctaccggcg ttcgtcccgg agataatcaa cggtttcttc gcacctgcca gtgcctgcac
     9241 gatgacgtcg attttgcttt gcagctcagg ttcgataccg tcaaccgctg gcgcagagtt
     9301 atccagcgca tgggcgatgg caaaacctaa acgcgcctga tcttcaaccg gtgcgcggta
     9361 agtccacgcc gcgatatcat ccagacgggt gtcatcaacg ttggtaacaa acagcggatg
     9421 cttcgcacgt tgaccgatgt tgaggattgc cgcaatctgc cagtcagcca ctttctgtgc
     9481 tgccgccatt tcgcgcgctt tacctttcac agcctgacgc actgccagcg cgacgcgcgc
     9541 gccggtctgg gtaacgtctt cgcccagcac cagtaccgca tcgtaagatt cgatttcgcg
     9601 cagagccgga gtataaatgc cgccttcacg cagcactttc agcgccagtt gcagacgttc
     9661 ctgctcaccg tgagcgatac cggtgtagaa gttttcttcg cccaccagtt cacgcagcgc
     9721 aaagttgctt tccacgctgg cacgcggaga accaataccg atcactttct tcgactgacg
     9781 cagaatatct gccgcgccct gcattgcctg ttcggcgttg agggtaatga aatcatcgcc
     9841 acgacgctgt actggctgac gcggacgatc cttcaggttg acgtaaccgt aaccgaaacg
     9901 accacggtcg cagaggaagt agtggtttac cgtaccgttg taacggtttt cgatacgacg
     9961 cagttcgccg taacgttcac cggggctgat gttacagccg atggaacatt gctggcagat
    10021 gctcggcgca aactgcatat cccatttacg gttgtaacgc tcggagtgcg ttttgtcggt
    10081 aaatacgccg gtcgggcaaa tttcgaccag gttaccagaa aattcgcttt ccagcgtgcc
    10141 gtcttccggg cgaccgaagt agacgttgtc gtgcgcaccg taaacgccca gatctgtacc
    10201 gtcagcgtaa tctttgtagt aacgcacaca gcggtagcag gcgatgcagc ggttcatttc
    10261 gtgagagatg aatggcccca aatcctgatt acggtgggta cgtttggtga aacggtagcg
    10321 acggaagctg tgtccggtca tcacagtcat atcctgaaga tggcagttac cgccttcttc
    10381 acataccgga cagtcgtgcg ggtggttggt cattaaccac tcgaccacac tttcacggaa
    10441 ctgtttcgct tcttcgtcgt caatggaaat aaaggtgcca tcggaagccg gtgtcataca
    10501 ggacatcacc aggcgaccac gcgtgtcttc cgcgttttgg tattgcttca ccgcacactg
    10561 gcggcaagca ccgacgcttc ccagcgccgg atgccagcaa aagtaaggaa tatcaaggcc
    10621 cagagacaga caagcttcca gcaggttgtc cgctccgttg acctcgtatt ctttgccgtc
    10681 tacatgaatt gtagccatta gcatgcttcc agttttctcg gtcagggacc gagcgttaat
    10741 cgaaattcgg ttaccagcgc tctttcagca ggttcggctg aatcccatta atcaaatggg
    10801 tattgctgaa cggctgtttg attcccgcct caaattcttc gcggaaatat ttgatggcgc
    10861 tctgtaacgg ctccactgca ccaggtgcgt gggcacagaa agttttaccc gggcctaaga
    10921 atcgacacag ttgctcaagt gtttcgatat cgcccggctg gccttcgcca cgctccagcg
    10981 cacgcagaat tttcacgctc cacggcagac cgtcgcggca cggcgtacac cagccgcagg
    11041 actcacgggc gaaaaactct tccaggttac gcaccagcga caccatgttg atctcatggt
    11101 caaccgccat cgccagcgcc gtacccagac ggctgcccgc tttaccgata ctttcgaatt
    11161 ccatcggcag atcaaggtgc gcttcggtca ggaagtcagt ccccgcgccg cctggctgcc
    11221 aagctttaaa tttcagacca tcacgcatac caccggcgta atcttcgagg atctcgcgtg
    11281 cggtggtgcc gaacggcagt tcccacagtc ccggattttt cacccgaccg gagaagccca
    11341 tcagcttggt gccagcatct ttacttttcg agatgttctg ataccactcc acgccgttag
    11401 cgaggatcgc cggaacgtta cacagggttt cgacgttgtt gacacaggtc ggtttgcccc
    11461 atacgccgga ggttgccggg aaggggggtt tcgagcgtgg gttagcacga cgcccttcca
    11521 gggagttgat taacgctgtt tcttccccgc agatgtagcg ccctgccccg gtatggacga
    11581 acagttcgaa gtcgaaacct gttcccataa tgtttttgcc aagcaagccc gcttcagtgg
    11641 cttcggcaat ggcgcggcgc agatttactg ccgcttcgat atattcgcca cgcaggaaga
    11701 tgtagccacg gtaagctttc agcgcaaacg cggagatgag cataccttcc accagcaggt
    11761 gcggcagttg ctccatcaac aggcggtctt tataggtgcc cggctccatt tcatcggcgt
    11821 tacacagcag gtaacggatg ttcatggatt cgtctttcgg catcaggctc cacttcaggc
    11881 cagtcgaaaa gcccgctccg ccgcgccctt tcagaccagc gtcttttacc tgattaacga
    11941 tttcgtccgg agacagcccg gtcagcgcct tacgcgcccc ttcgtaaccg tttttgctgc
    12001 ggtattcgtc cagccacact ggctgtttgt catcgcgcag acgccaggtc agcggatgcg
    12061 tttcgggagt acggataatg tttttcattt ataccgctcc agcagttcag ggatcgcttc
    12121 cggggtcaga tgcgcgtgag tgtcctcatc gatcatcatg tttggccctt tatcacagtt
    12181 ccccaggcag caagttggca gcagcgtaaa gcggccatca aatgtcgttt gccctggttt
    12241 gatgttcagc tttttctcga gcgccgcctg aataccctga tacccgttga tatgacagac
    12301 cacgctgtcg caataacgga tcacatggcg accaaccggc tggcggaaga tctgactgta
    12361 gaacgttgcc acaccttcga cgtcgcttgc cggaataccc agcacatcgg cgatcgcgtg
    12421 gatcgcacca tccggcaccc agccacgctg cttctgaacg attttcagcg cttcaatgga
    12481 cgccgcacgc gggtcttcgt agtggtgcat ctcgtgctca atcgcttcac gctctgccgc
    12541 actcagctca aaagcctcgg tttgtggttg ttgattctcg tgcataatta gcggtccaca
    12601 tctgacataa caaaatcgat actgcccaga taaacaatca ggtcagacac caggctgccg
    12661 cggatcgccg ccggaatttg ctgcaaatgc gcataactcg gcgtgcggat acgagtgcga
    12721 taactcatgg tgctaccgtc gctggtcagg tagtaactgt tgatcccctt ggtcgcctca
    12781 atcatctgga aagattcatt ggcaggcatc accggccccc acgacacttg caggaagtgg
    12841 gtgatcaggg tttcgatatg ttgcagcgtg cgctctttcg gcggcggcgt ggtcagcggg
    12901 tgatccgctt tgaacgggcc ttccggcatg ttgttgaggc actgctcaag aatgcgcaga
    12961 ctctggcgca gctcttccac tttcagcatt acgcgggtgt agcagtcaga aacaccacct
    13021 ccaaccggga tttcaaagtc gaagttttca tagccagaat aaggacgcgc cttacgcacg
    13081 tcgaagtcga tcccggtagc acgcaggccc gcgccagtgg tgccccactc cagcgcctct
    13141 ttcgcgccat aggcggcaac gccctgggaa cgacctttca gaatggtatt ttgcagcgcc
    13201 gctttctcgt aagacgccag acgtttcggc atccagtcga ggaactcacg cagcaggcga
    13261 tcccagccgc gcggcaggtc gtgcgctacg ccgccaatac ggaaccacgc cgggtgcata
    13321 cggaaacccg tgatcgcttc caccagatcg taaattttct gacgatcggt aaaggcgaag
    13381 aacacgggcg tcattgcgcc gacgtcctga ataaaggtcg agatgtacag caggtggctg
    13441 ttgatacgga acagttcgga gagcataacg cgaatgacgt taacgcgatc cggcacggtg
    13501 atcccggcca gtttctctac cgccagcacg taaggcattt cgttaacgca gccgccgagg
    13561 tattcaatac ggtcggtata cggaatgtag ctgtgccagg actggcgctc gcccattttc
    13621 tccgcaccac ggtggtggta gccgatgtct ggtacgcagt cgacaatctc ttcgccgtcg
    13681 agttgtaaaa cgatacggaa agccccgtgc gccgacgggt ggttcggacc gaggttgagg
    13741 aacatgaagt cctcgttttc ggtgccgcgc ttcatccccc actcttccgg tttgaaggtc
    13801 agggcttcca tctccagatc ctgtttggct ttggtcagct caaacggcga gaattcggta
    13861 gcgcgcgccg ggtaatcttt acgcagcggg tgacctttcc aggtttgcgg catcatgatg
    13921 cggcgcaggt tcgggtgacc gtcgaaagta atgccaaaca gatcccaggt ttcacgctca
    13981 taccagttag cgttcgggaa cagtttggtg aaggtcggta cgtgcaggtc gttttctgcc
    14041 agcgccacct tcagcatgat gtcgcggtta cgatcgatag aaatcagatg gtagaaaacg
    14101 gaaaaatccg cggcaggtaa cccttcgcgg tgtgtgcgca gacgttcgtc catgccgtgt
    14161 aagtcaaaca gcatgacgta aggtttcggc agtttcttta agaaatcgcc aacttccagt
    14221 aattgttcac gcttgatcca cacaacggga accccggtgc gagtcgcctg aacagtaaag
    14281 gcatccggcc caaaacggtt gcgcagttcg ccaatcaccg gatcatcaag atgatcacgg
    14341 gtctgccagg cgggttcttg cgcggttaag tcggtcatat tgttcaccat tgcaaatggt
    14401 ccgtggtgac tgtcggcgca gtaagcttcg cgaaatagat ttaggaataa gcgaagaaaa
    14461 atccctttgc cgacaggcgc aaattaaatc tcgtcaggtg tacgcaggtt ggttacggca
    14521 atgcgttcac cgcgcttgcg ttcgcgctct gattgcatat tggcgcgata aacgccctga
    14581 tcgccaacca cccaggagag cggacgacgt tctttgccga tagattcctg caacagcatc
    14641 agtgcctgca tgtacgcttc aggacgcggc gggcagcccg ggatatacac atcaaccggg
    14701 atgaatttat cgacgccctg cacaacggaa taaatatcgt acataccacc agagttggca
    14761 caggcaccca ttgagataac ccattttggt tccagcatct ggtcatacag acgctgaata
    14821 accggtgcca ttttggtaaa gcaggttcct gcaaccacca tcaggtcagc ctgacgcggc
    14881 gaagcacgca atacttctgc gccaaaacgc gccacgtcat gcaccgcggt aaacgaagtc
    14941 accatctcaa cgtaacagca ggaaagaccg aagttatacg gccaaattga gtttttacga
    15001 ccccagttaa ccatgtcatt gagcttgccc ataaacacgt ttttgttaac ttcttgctcc
    15061 agagggtcgg ttacgatctc ctgcttttgc agggggtaac ggtcgttctc accgttggga
    15121 tctatgcggg tgagcgtata atccatctta atgcctcgcg gttagcgttg acgattagcg
    15181 atactgttcg tttccgggtt catacgctcg cggcgtgaac gcgcgggcgt ccagtccagc
    15241 gcgccaatac gcaccagata aaccagacct gccagtaaca caaaaataaa aattgcagct
    15301 tccacaaagc ctacccagcc gctttcgcgg atagaggttg accatgcgaa cagatacagc
    15361 gcttcaacgt cgaagataac gaagaacatg gccaccagat aaaacttggc ggacaggcgt
    15421 aagcgggcgg agccgaccga gtcgataccg gattcaaacg gcacgttttt cgacctcgcg
    15481 cgtgcgcgac cgcctaaaaa ccaaccgcct accagcatca ggcaacacag gccaatggca
    15541 acgataagaa agatagcgaa tgcccagtga tgagcgatga cttcagtgga tgttgacata
    15601 ctcattgctt actcatcaaa agtagcgcca gattcactgc tcttcacggc agatggacgc
    15661 cacatcgatt catggggagg aataaaaaaa accttacaat cactgtagaa attcttttat
    15721 acagctaatt gatgtggtct tttactcctt tctataacct tttgtcaact ttaacaaaag
    15781 tttcttcaca ttagtttaca taatatcaac accattagca tttaatgccc tttcacgcca
    15841 gatccttgac gactccagga taattagatg ttgttgaatc gtgtccgttg tgaagcaatg
    15901 gaaaaaatac gggtctattt tgacaggaat ttgtgtcgat tcctcccccc aaaagagagt
    15961 attttcttga tctgtgacac gcttttgtca ttccataaca aaaacgcagc aacaaattta
    16021 cgcattttta acatcattgt agcaggtgat ttttttcagg cgattatttg tgcgttcggg
    16081 acgtgaatct ctggtgattg aaaaatgaac agttttgtac gttctgcact atgcgaggaa
    16141 ggatttttac taaaaaaaag ccgctggggt ttaaaacacc cccagcggct cgtttttaca
    16201 ctattgtctc aggaattatc tatcgtccgt cgattactcg atatcccttt caatcaacaa
    16261 tgaatcatcc ccttccggag cagacattgg gctgtattgc cacggattat ggtagctctc
    16321 catcgcctga tagatcacct gggccagctc attattactg gacgggtcat agcacagcaa
    16381 atattcggta tcaggtagcg gcggtaagcc atctactcca ctcagcacac gcaggtacgg
    16441 gctcatcatt tcaatcggcc ttgccgtcac gccaagaccg gctttcactg ccgcacgaac
    16501 ggccggaagc gtcgaggcga cataagccag tcgccatgga atatctgctt tattaagcgt
    16561 cgccagcacc atatcgcgaa acggacttgg atcatccagc aatacaagag ggatcggctc
    16621 gcctttttgc aatacgtatt ccgctgcgca gtaccagtgt gttggcgagg tacgcaggtt
    16681 caatgcctta aacgctgaag gacgatgggt ggttaccatc aaatccactt cctgggattc
    16741 cagcatttct gccatatagg cattacgttt aacgcggaca tccagcgcta atttcggata
    16801 aaccgaactc acgcgattta acaggaaagg taagatcgta tcggcagatt catctgaagc
    16861 accgatagtt aacacgccct gaagattact gaacattaat gatgagcagg cctcatcatt
    16921 aaaacgcagt attttcctgg cgtaaccaag aagttgaatg ccatgttcag ttaacagttt
    16981 gttgcgaccg tgacgagcga acagttcttt cccaacgagt tgttccagac gctgcatttg
    17041 ctgacttacg gcggactgag tacgacacac agcggcagct gcggcagcaa aagtgttcag
    17101 atcggcaaca gcaacaaatg ttctcagcag atcgaggtcg aggttaatta tcggacgatt
    17161 tgcacttatc atatattatc acttactggc ggctcatact gagctggtta atgctgtgca
    17221 cacacaaaca agcaattcca tttgtaatgt gcctccctgg cagtttcatc cggaacccgg
    17281 acgaaagtaa aaatgcatat gagttgcact aaaaaagcga ctcacattgt tccgttataa
    17341 tgcctgaagt agatcacaga atatatcttc agggatcgca tatctattaa gttactcact
    17401 cttttctatt tatgacatgc gcgtgtttgt ataaatgtaa atgtgagtcc ttgttccact
    17461 ctcgtgcagc atcgctggtc atacgcgaac acgtacaaac agcaatggtg aggcggcatc
    17521 aagagcaggg atccgtcatt tatgcgagca ttttacccca aaaacctttt atttataagg
    17581 gtcattgcga attatctgat gcaaagttat gttaggcaaa gtaatcttct attattaata
    17641 agcacatcaa aacctttttg aatattaaat aataattaat tagcatcatc ctcattcatt
    17701 aattccgctt aacaatagtt tcacaccttg cccccattgc gacacctcgg aaatcatcac
    17761 gctgttaaac tctccgtaat gcggaaatat catcatcgcg tttcattagt gaattcttaa
    17821 atgaggcatt ttcacacaat tatcttacag ataaaaaaac cagacttgca attaagaatc
    17881 agaacaattc actatataac attgcatgca aagcatatac gcctcattat tttgtcatta
    17941 ttaagttatt aacagcacaa tcgagccttc ccctctggca aaatcttatt ctgcagacct
    18001 tcaaaacacc gtcctggggg agtacattgt tctaagctga cttccacggc agggagtggc
    18061 gataacagca aaaaaggtca agattcatgt cccccattga aaaatccagc aaattagaga
    18121 atgtctgtta tgacatccgt ggtccggtgc tgaaagaagc aaaacgcctg gaagaagaag
    18181 gtaacaaggt actgaaactg aacatcggca acccagcccc attcggtttt gacgcgccag
    18241 atgaaatcct cgttgacgtg atacgcaacc tgcctaccgc tcaagggtat tgcgattcca
    18301 aagggcttta ctccgcgcgt aaagccatca tgcagcacta ccaggctcgt ggcatgcgtg
    18361 atgttaccgt ggaagatatt tacatcggca atggtgtatc ggagcttatc gttcaggcaa
    18421 tgcaggcatt gctgaacagc ggggacgaaa tgttggttcc tgcaccagat tacccactct
    18481 ggaccgcggc ggtttcgctt tccagcggta aagcggtgca ttatctttgc gatgaatcct
    18541 ctgactggtt cccggacctc gatgatattc gcgctaaaat tacgcctcgt acgcgtggga
    18601 tcgttattat caacccaaat aacccaaccg gcgcggtata ttccaaagag cttttaatgg
    18661 agattgtgga gattgcacgt cagcataatc tcattatctt cgccgatgaa atttatgaca
    18721 aaattctcta cgacgatgct gagcatcact caattgcgcc actggcacct gacctgctga
    18781 ccattacctt taacggactg tcgaaaacgt accgcgttgc aggcttccgt caggggtgga
    18841 tggtgttgaa cgggccgaaa aaacacgcca aaggctacat cgaaggtctg gaaatgctgg
    18901 cttcaatgcg cctgtgtgct aacgttcctg cgcaacacgc cattcagacc gcgctgggtg
    18961 gttatcagag catcagtgaa tttattaccc ctggcggtcg tctttatgag cagcgtaacc
    19021 gcgcgtggga actgatcaac gatattccgg gcgtttcctg cgtgaaacct cgtggtgcgc
    19081 tgtatatgtt cccgaaaatc gacgccaaac gctttaacat tcacgacgat cagaaaatgg
    19141 tgctggattt cctgttgcag gaaaaagttc tgttggtgca agggacggca ttcaactggc
    19201 cgtggccgga tcacttccgc attgtcacgc taccgcgtgt cgatgatatc gagctgtctt
    19261 tgagcaagtt cgcgcgtttc ctttctggtt atcatcagct gtaatcttaa tttcactgcc
    19321 ggagattgca tccggcagcg ttatcccgcc acaatgacct gatgatgtca tcatacgtaa
    19381 ggtcactatg aaacagagcc atttttttgc ccatctctcc cgcctgaaac tcattaaccg
    19441 ctggccgcta atgcgcaacg tgcggacgga aaatgtgtcc gaacacagtt tgcaggtagc
    19501 gatggtcgcc catgcgctgg cagctatcaa aaatcgaaaa tttggcggta atgtcaacgc
    19561 cgaacgtatc gctttactgg cgatgtacca cgatgcctca gaagtgctca ccggcgatct
    19621 ccctactccg gtgaaatact tcaattcgca aatcgctcag gagtacaagg ctattgaaaa
    19681 aatcgctcag caaaaactgg tcaatatggt tccggaagag ctgcgggata tctttgcgcc
    19741 gttaattgac gagcatgcat atagcgatga agaaaaatcg ctggtgaaac aggcagatgc
    19801 actgtgtgca tatctgaaat gtctggaaga actcgcggcc ggaaataatg aattcttgct
    19861 ggcaaaaacg cgactggaag cgacgcttga agcgcgtcgc agccaggaga tggactactt
    19921 catggaagta tttgttccca gcttccatct ttcgctcgat gagattagcc aggattcacc
    19981 gctgtaagca gccggagtct gcgtcgcatc aggcaataag cgccggatgc gacatcaggc
    20041 tcttgtcaaa acggaaacag catcgggatc atcaccacac aaaccgccat cacgataatg
    20101 gtgaacggta cccccaactt cacaaagtca ctgaagctgt aatttcccgg acctaaaacc
    20161 agtgtgttaa caggtgaaga aaccggtgtc ataaaggcgg cggatgctgc catcgccacg
    20221 accatcgcga atggataagg cgacaccccc atcgttttgg cagcagccag cgcaatcgga
    20281 gccatcaaca ccgccgtcgc ggtattagag ataaatagcc caataaccgc cgacaagaca
    20341 aacaaacacc ccagcatcat atgtggcccg taaccaccgc caatgtccat cagccctttc
    20401 accgccagcg cgacacctcc cgttttctgt aatgccacag caaacggcat catcccaacg
    20461 atcaaaataa tgctcggcca gtgaatggat ttataggcgc tttcagcatc tatacagcgg
    20521 aatttcccca tcagcaggca ggcgatgata gcggcaacag gattaggaat ttcatctgtc
    20581 agcattaacg ccaccattag caccagacag aaaatggcat ggggtgcctg gctgtgcgcg
    20641 ggcgatgctt cactcacctc ttccggtaag ttcagcgcta cgaagtcgcg gccctgtttg
    20701 gccagcatac cgatcagttt ccagttaccc acaaccagga tgatatcgcc cagcagcaga
    20761 ggctcatccg ccagcgaacc ttccagcgcc acgccattgc gctttagccc caccacattc
    20821 agtccgtagc gggtacgaaa accaatttcg cgcaccgatt taccaatcag ttctgactca
    20881 ggaattaatg aaatctctgc catgcccaca tcaagggcct ggtcagaaaa atactcgccg
    20941 cgcagtacca tcggctccag caattgctca ctacaaaatt gccggagatc gacatcagcc
    21001 gcagacatat caataagcaa aacgtcacgc gcgcgaaatt cagaaacccc attaacgttc
    21061 acgataacgc gacgaaaacg ccgccagcgt tcaacaccga tgacgttagc gccataacgc
    21121 tcacgtaatt tgagatcatc cagccgttga ccaatcattg gcgatccggg gcgaatagcc
    21181 agacgtcgcg cacgcccggt cagtcgatat tcacggataa gatcgcgaaa ggttcgacgc
    21241 gtccagcctt cgcgctgcgg ggtctgggta tcccctttca gcatgaaacg catcactaac
    21301 atatacaaaa tacccagcac cagcacgacc aggccaatag gtgttacgct aaagaaactg
    21361 aagccgtgat agccttcacg cagcaattca ctgttgacta ccaggttcgg cggcgtcgcc
    21421 accagcgtca tcatgccgct tatcagcccg gcaaaactca acggcatcat cagacgtgac
    21481 ggcgaagttt gcatacgcat ggcaacgctt aacaccacgg gaataaagat agcgacaacg
    21541 ccggttgaac tcataaacgc gccaagcccc gcgacggtca gcatcaacaa aaccaacatt
    21601 ttgatttcac tgttgcccgc aactttgacc agccatgttc ccattacggt ggcaacaccg
    21661 gtacggacca aaccatcgcc aataataaac aaggcggcaa tcaggacaac gttaggatca
    21721 gaaaagccgg aaaatacttc ggggactgtc agcgttccgc ttaatgcaaa cgcgacaata
    21781 acaaacaaag cgaccgcatc catacgcact ctgcccgtcg caaacaagac aatggcaacg
    21841 gccagtaatg aaagaaccca aatcaattca ccgttcacaa cttatccttg ttaattgagg
    21901 gagatgtctt gattctgcca taaaaaagcc ccgacgagac ggggctaaat catgatcagg
    21961 tgtttcactg aataataaca tcgccatttg gctgtttggt cacagtaatt tgctccagac
    22021 tgtggaggac caaatcgacc tcattcaggc gcggggtatc tgccggagcg ttaaccgcaa
    22081 tgacatgaca acccgccgcc aggccagaaa gcacgccagc ggagcatctt ccaccaccac
    22141 acactcctgc ggcgcaagcc ccagcagctg cgcgcctaac agatacgcat caggttctgg
    22201 ttttccgcgc ttcactcgct cagcggttac aaacacctct ggtgcgggaa gcccagctat
    22261 tttatggcgc gctcgcgcta ccggcatgga gccagaagtc acaatggccc acggaatacc
    22321 tgctttattc aaatgactga gtaaggcgat tgcccccgga agcgggtaat accttcggtt
    22381 tccgtggcct cgatgtgctc cagacgcgta aactcggcgg caatatcagc ctcggatttg
    22441 cccgccataa aatggcgcag agaggtgatc gcctgtttac cgtgaatgaa agccagcacc
    22501 tcttccggcg ctaacccatg acgtctggcc cagttgctcc acgcccgttc taccgcaggc
    22561 agggaatcca ccagcgttcc atcaagatca aacagaaaac ctttgcaccg cacgcgggcc
    22621 tcctcaggca ttgataattt gattaatttc gttggcgctc aaatggtact gacgcgggca
    22681 ggcatgccac acattaagca tgcgctgata tttttcccac attggcgtct gggcgttaaa
    22741 accgtgagtt ccggcgtcaa aatgggtata gcgcccttcc acattaacca taaagcggac
    22801 ataaccgagg taacgtgctt cagtggcggc gtcaaagccg aggaaggtga cacgacgttc
    22861 atcgatggat tgctggtcct gcaaattaga ccaggaaaca tgcaacgcat gatacatctc
    22921 cataatgtcg atgatagtgc ggcaggtttc ttctttcagc tcgccaaact cgcgatccaa
    22981 ttcacgcatc tgtaatccgt aacctcgctc aataattgtt tgcaggcgac ggtaacgttc
    23041 agcatttgcc ggatcgagca tagtcatcat cttgtactga ttagacaaaa taagacgttg
    23101 cgcgttggtc atttccattg ttgactcctg tatcactcta ctacggtgaa aaaaaagaag
    23161 gctgagtatg ccttctttta tatgcgtaat caggggtcaa ttacaaatca tcaaggaaag
    23221 ttttatccag ttgtttgaag gcgcgcttaa gcgtgtcagc taatgcctgg taatcaggct
    23281 tgccttcaac gggtgccaac acctgtccag actcctgcaa tttaccgcga acttcataaa
    23341 accagttaag gattgcaggg ggtaatggcg ttacagaacg cttgcccagc caccacaatc
    23401 cctgcatggg taaacttaag gcgaacagtg cagtggcaac tgccggccca agctgaccgc
    23461 ccagggcaat ctgccagcag agagtaaata cggcgatcgg cggcataaaa cggatcgcat
    23521 aacgcgtcat cttgataacg cgattttcga caaagaccgg ggcaaggcgt ttttccagtg
    23581 gccacgtctt tgagtaatgc tgtccgcggc gaaacaagct aaaaaaatta acagaacgat
    23641 tatccggcgt tgacatgctt cacctcaact tcacatataa agattcaaaa atttgtgcaa
    23701 attcacaact cagcgggaca acgttcaaaa cattttgtct tccataccca ctatcaggta
    23761 tcctttagca gcctgaaggc ctaagtagta catattcatt gagtcgtcaa attcatatac
    23821 attatgccat tggctgaaaa ttacgcaaaa tggcatagac tcaagatatt tcttccatca
    23881 tgcaaaaaaa atttgcagtg catgatgtta atcataaatg tcggtgtcat catgcgctac
    23941 gctctatggc tccctgacgt ttttttagcc acgtatcaat tataggtact tccatgtcga
    24001 gtaagttagt actggttctg aactgcggta gttcttcact gaaatttgcc atcatcgatg
    24061 cagtaaatgg tgaagagtac ctttctggtt tagccgaatg tttccacctg cctgaagcac
    24121 gtatcaaatg gaaaatggac ggcaataaac aggaagcggc tttaggtgca ggcgccgctc
    24181 acagcgaagc gctcaacttt atcgttaata ctattctggc acaaaaacca gaactgtctg
    24241 cgcagctgac tgctatcggt caccgtatcg tacacggcgg cgaaaagtat accagctccg
    24301 tagtgatcga tgagtctgtt attcagggta tcaaagatgc agcttctttt gcaccgctgc
    24361 acaacccggc tcacctgatc ggtatcgaag aagctctgaa atctttccca cagctgaaag
    24421 acaaaaacgt tgctgtattt gacaccgcgt tccaccagac tatgccggaa gagtcttacc
    24481 tctacgccct gccttacaac ctgtacaaag agcacggcat ccgtcgttac ggcgcgcacg
    24541 gcaccagcca cttctatgta acccaggaag cggcaaaaat gctgaacaaa ccggtagaag
    24601 aactgaacat catcacctgc cacctgggca acggtggttc cgtttctgct atccgcaacg
    24661 gtaaatgcgt tgacacctct atgggcctga ccccgctgga aggtctggtc atgggtaccc
    24721 gttctggtga tatcgatccg gcgatcatct tccacctgca cgacaccctg ggcatgagcg
    24781 ttgacgcaat caacaaactg ctgaccaaag agtctggcct gctgggtctg accgaagtga
    24841 ccagcgactg ccgctatgtt gaagacaact acgcgacgaa agaagacgcg aagcgcgcaa
    24901 tggacgttta ctgccaccgc ctggcgaaat acatcggtgc ctacactgcg ctgatggatg
    24961 gtcgtctgga cgctgttgta ttcactggtg gtatcggtga aaatgccgcg atggttcgtg
    25021 aactgtctct gggcaaactg ggcgtgctgg gctttgaagt tgatcatgaa cgcaacctgg
    25081 ctgcacgttt cggcaaatct ggtttcatca acaaagaagg tacccgtcct gcggtggtta
    25141 tcccaaccaa cgaagaactg gttatcgcgc aagacgcgag ccgcctgact gcctgatttc
    25201 acaccgccag ctcagctggc ggtgctgttt tgtaacccgc caaatcggcg gtaacgaaag
    25261 aggataaacc gtgtcccgta ttattatgct gatccctacc ggaaccagcg tcggtctgac
    25321 cagcgtcagc cttggcgtga tccgtgcaat ggaacgcaaa ggcgttcgtc tgagcgtttt
    25381 caaacctatc gctcagccgc gtaccggtgg cgatgcgccc gatcagacta cgactatcgt
    25441 gcgtgcgaac tcttccacca cgacggccgc tgaaccgctg aaaatgagct acgttgaagg
    25501 tctgctttcc agcaatcaga aagatgtgct gatggaagag atcgtcgcaa actaccacgc
    25561 taacaccaaa gacgctgaag tcgttctggt tgaaggtctg gtcccgacac gtaagcacca
    25621 gtttgcccag tctctgaact acgaaatcgc taaaacgctg aatgcggaaa tcgtcttcgt
    25681 tatgtctcag ggcactgaca ccccggaaca gctgaaagag cgtatcgaac tgacccgcaa
    25741 cagcttcggc ggtgccaaaa acaccaacat caccggcgtt atcgttaaca aactgaacgc
    25801 accggttgat gaacagggtc gtactcgccc ggatctgtcc gagattttcg acgactcttc
    25861 caaagctaaa gtaaacaatg ttgatccggc gaagctgcaa gaatccagcc ctctgccggt
    25921 tctcggcgct gtgccgtgga gctttgacct gatcgcgact cgtgcgatcg atatggctcg
    25981 ccacctgaat gcgaccatca tcaacgaagg cgacatcaat actcgccgcg ttaaatccgt
    26041 cactttctgc gcacgcagca ttccgcacat gctggagcac ttccgtgccg gttctctgct
    26101 ggtgacttcc gcagaccgtc ctgacgtgct ggtggccgct tgcctggcag ccatgaacgg
    26161 cgtagaaatc ggtgccctgc tgctgactgg cggctacgaa atggacgcgc gcatttctaa
    26221 actgtgcgaa cgtgctttcg ctaccggcct gccggtattt atggtgaaca ccaacacctg
    26281 gcagacctct ctgagcctgc agagcttcaa cctggaagtt ccggttgacg atcacgaacg
    26341 tatcgagaaa gttcaggaat acgttgctaa ctacatcaac gctgactgga tcgaatctct
    26401 gactgccact tctgagcgca gccgtcgtct gtctccgcct gcgttccgtt atcagctgac
    26461 tgaacttgcg cgcaaagcgg gcaaacgtat cgtactgccg gaaggtgacg aaccgcgtac
    26521 cgttaaagca gccgctatct gtgctgaacg tggtatcgca acttgcgtac tgctgggtaa
    26581 tccggcagag atcaaccgtg ttgcagcgtc tcagggtgta gaactgggtg cagggattga
    26641 aatcgttgat ccagaagtgg ttcgcgaaag ctatgttggt cgtctggtcg aactgcgtaa
    26701 gaacaaaggc atgaccgaaa ccgttgcccg cgaacagctg gaagacaacg tggtgctcgg
    26761 tacgctgatg ctggaacagg atgaagttga tggtctggtt tccggtgctg ttcacactac
    26821 cgcaaacacc atccgtccgc cgctgcagct gatcaaaact gcaccgggca gctccctggt
    26881 atcttccgtg ttcttcatgc tgctgccgga acaggtttac gtttacggtg actgtgcgat
    26941 caacccggat ccgaccgctg aacagctggc agaaatcgcg attcagtccg ctgattccgc
    27001 tgcggccttc ggtatcgaac cgcgcgttgc tatgctctcc tactccaccg gtacttctgg
    27061 tgcaggtagc gacgtagaaa aagttcgcga agcaactcgt ctggcgcagg aaaaacgtcc
    27121 tgacctgatg atcgacggtc cgctgcagta cgacgctgcg gtaatggctg acgttgcgaa
    27181 atccaaagcg ccgaactctc cggttgcagg tcgcgctacc gtgtttatct tcccggatct
    27241 gaacaccggt aacaccacct acaaagcggt acagcgttct gccgacctga tctccatcgg
    27301 gccgatgctg cagggtatgc gcaagccggt taacgacctg tcccgtggcg cactggttga
    27361 cgatatcgtc tacaccatcg cgctgactgc gattcagtct gcacagcagc agtaacctcg
    27421 tcatcatccg cagctttgcg ctgcggatat ctgaaccgga aataatcact atttccggtt
    27481 ttttattctc ttaatctgca ttaatccttt ctgattatct tgcttaactg cgctgcatca
    27541 atgaattgcg ccatttcact ttacatactt accactttgt tttgtgcaag ggaatatttg
    27601 cgctatgtcc gcaatcactg aatccaaacc aacaagaaga tgggcaatgc ccgatacgtt
    27661 ggtgattatc ttttttgttg ctattttaac cagccttgcc acctgggtag ttccggtggg
    27721 gatgtttgac agtcaggaag tgcagtatca ggttgatggt caaacaaaaa cacgcaaagt
    27781 cgtagatcca cactcatttc gcattctgac taacgaagca ggcgaacctg agtatcaccg
    27841 cgtacagctg ttcacgacgg gcgatgaacg cccgggcctg atgaacttcc cgtttgaagg
    27901 attaacctca ggatcgaaat acgggacagc tgttggcatc atcatgttta tgctggtgat
    27961 tggcggcgcg tttggcattg tgatgcgtac aggaaccatt gataacggta tcctggcgct
    28021 tattcgccat acccgcggga atgaaattct ctttattcct gcgctgttta ttctgttttc
    28081 acttggcggc gcggtatttg gtatgggaga agaggccgtc gcctttgcca ttatcatcgc
    28141 accgctaatg gtccggctgg gctatgacag tattaccacc gtcctggtga cctatattgc
    28201 cacgcaaatc ggttttgcca gttcgtggat gaacccgttt tgtgtggtcg ttgctcaggg
    28261 gattgccggc gttccggtgc tttctggctc cgggttgcgc atcgtggtgt gggttatcgc
    28321 cactatgatt ggcctgatct ttaccatggt gtacgcctca cgagtgaaaa agaatcctct
    28381 tctgtcacgc gtgcatgagt ccgaccgctt ctttcgtgaa aagcaggcgg atgttgaaca
    28441 acgtccgttt acctttggtg actggctggt attgattgtc ctgaccgccg taatggtctg
    28501 ggtgatttgg ggcgtgatcg ttaatgcctg gtttattcca gaaattgcca gccagttctt
    28561 caccatgggt ctggtgattg gcatcatcgg cgttgttttc cgccttaacg gcatgacggt
    28621 taataccatg gcttcatcct ttaccgaagg gacgcgaatg atgatcgccc ctgccctgct
    28681 ggtgggtttc gccaaaggga ttttgctgct ggtcggtaat ggtgaagcgg gtgatgccag
    28741 cgtgttaaat accatcctca acagcattgc caatgccatt agcggtctgg acaacgcggt
    28801 cgcggcctgg tttatgttgc tcttccaggc ggtatttaat ttcttcgtga cgtccggttc
    28861 tggtcaggcg gcgttaacca tgccgttact ggcaccgctt ggcgatctgg tcggtgttaa
    28921 ccgtcaggtt accgtgctgg cattccagtt tggtgatggc ttcagtcaca tcatttaccc
    28981 aacctcagct tcgttaatgg caactctcgg tgtgtgcagg gtggacttcc gtaactggct
    29041 gaaggtgggc gcgaccctac ttggactgct gtttattatg tccagcgtcg tagtgatcgg
    29101 cgctcagttg atgggctacc actaaaaatg ttaagagccg caattgcggc tcttttttca
    29161 ttctgccgtt tcagtctcta cggcttcatt tttggcattg cgcttcatcc acaacgccag
    29221 cgctttcagc gagtctggag tgaactcatc gcagcgtgcg gtgatttctt ccggcgtcag
    29281 ccagcaaact tcactgactt catcttcctg tagggcgaag ggaccgtgag agacgcagct
    29341 gaacaacgcg ccccagacac ggcaattttt atcttcgaaa tagaactgcc cgtgctcggc
    29401 aaaggggaca ccggcaatgc ccaactcttc ttccgcttcg cgacgcgcgg attccagcag
    29461 ttgctcatcg gcctgaacca caccgcctgc ggtcgcatct aacatgccgg gtaaaaagtc
    29521 ttttgtctcg gtacgacgct ggaccagaat tttgcccatg ccatcatgca cgacgatgta
    29581 agttgcacga tgacgcagac actgtgcccg catttgttcc cggctggctt gtgcaatgac
    29641 ttcgttctct tcattgacaa tatccaccca ttcagtactt gccaaacgac gctgttccat
    29701 tatcaggaaa ccttcttttt ctggcgctct tacggcgcat tttgagttgt gggtaaatta
    29761 cggattaatc gcgacctgcg caatgatact ttgatcattg agtgcgatta cactaagtac
    29821 gtcattatcc agcatgccat aactcgccgg attaccgcct ttcggaatac tcaccgagcc
    29881 ggggttgaag tggaaaatct ccccccgctg ttctgccact ggcagatggg tatgaccgta
    29941 caccagcaca tcgttctggt ttaaagcagg tagattttcc gggccaaaaa gatgaccatg
    30001 cgtcaaaaac agacgttgtt tttccagtaa tacctgttgc cacggcgcgg ttatcgggaa
    30061 atgcagcagc atttgatcca cttcgctgtc gcagttgccg cgcacagcga taaccttatg
    30121 tgccacttca ttaagccgtt cagcgacttt ggctggcgcg taaccctccg gtaaagcatt
    30181 acgcgggccg tgattcaaca cgtcgccaag gatcaccagc cactgggcac cgctttgggc
    30241 aaacaactcc agaacacgtt ccgtcgccgg taacgaccca tgaatgtccg atgcaaacat
    30301 cagtttcatc actcactcct cgtcgaaaaa acgtaagcta tgatactgga ttctgtcgtt
    30361 gctatcagcc agattgcttc gccgaaagtg caataaaacg ctggacagac gctcgctgcc
    30421 actggaatgt cgcataatcc accaggcgtt ctggcacctc atcgccatgt agtaccaggc
    30481 ggttaatcat tagcgccaga tcagtatcag caatgcacca ttcaccaaat aaattcggct
    30541 gacccagtgc taacaaatgt tctgccatcg cgaacagttt ctctgcactg gcttttccct
    30601 cggccgttag tggcgctttt ttcgcccccg caaagacaac atccgtcgga cgctcttcgc
    30661 ggatgggcat cagatcgctg cgcagccagg cctgaatccg tcgtgcacgc gcacgatttt
    30721 ctaaatcaag cggataaata cgttcccagg tcggtggcgc aaatcgatct tccagatact
    30781 ccgcaatggc agaagattca ctcaactcaa aatcatcgat ttgtaataac ggcacacggc
    30841 gtgtctgacc gtaaccttgc cacgtcggct tcaaatgttc accgctgtcg aggtcgatgg
    30901 tcttgatatg aaacgacagg cctttttcct gcaacgccac ccaggcggat aacacataag
    30961 gggagaaaaa gtgggcatct gaccaaagcg tgatagcggg tttactcatt atgtcctcgg
    31021 caataaaggg ggctggctat caaactatag agcctttttg tcactgtcac gtgacgaata
    31081 ctcacgtaag tcgccgcgct ttctatactt gtggtgttca gatgattaaa caaccggagc
    31141 tgcaatgatc gatctctatt tcgccccgac acccaatggt cacaaaatta cgctgtttct
    31201 cgaagaagca gagcttgatt atcgcttgat taaggtagac ctggggaaag gcggtcagtt
    31261 tcgtccggaa tttttgcgca tttcgcccaa caacaaaatt ccggcaattg ttgatcattc
    31321 tcctgccgat ggcggcgaac cgctaagcct ctttgagtct ggtgccattt tgttgtatct
    31381 gactgagaaa acaggactct ttttgagtca tgaaacgcgt gagcgcgccg ccacattaca
    31441 gtggttattc tggcaggtag gcggactggg gccgatgctt gggcaaaatc atcattttaa
    31501 tcacgcagcc ccccaaacca ttccttacgc tattgaacgt tatcaggttg aaactcagcg
    31561 tctttaccat gtactaaaca agcggctgga aaactcgccc tggctgggag gcgagaacta
    31621 cagcattgcg gatattgcct gctggccgtg ggttaatgcc tggactcgcc agcgaattga
    31681 cctcgcaatg tatccggcag tcaagaactg gcatgagcgg atccgttcgc gccctgccac
    31741 cgggcaggca ctgctaaaag cacaactcgg tgatgagcgt tcggatagtt aacagaaaca
    31801 ggttctcgtg tattatttca tcctaagtaa aacaacggag aacctgcaat ggcacaacct
    31861 gccgctatta ttcgtataaa gaaccttcgt ttacgtacgt ttatcggaat taaggaagaa
    31921 gaaattaaca accgtcagga tattgttatc aatgtgacga tccactaccc cgccgataaa
    31981 gcgcgcacta gcgaagatat caacgatgcg ctgaattatc gcaccgtaac gaaaaacatt
    32041 attcagcatg tagagaataa ccgtttctct ttgttggaaa aattaactca ggatgtgctc
    32101 gatatcgcac gtgaacatca ctgggtgacg tatgctgaag tggagatcga taaactgcac
    32161 gcgctgcgct acgccgattc ggtatccatg accttaagct ggcagcgtta accgccatat
    32221 cgggaggctg catgaacata gtgatcaccg gagggacggg attaattggt cgccatttga
    32281 ttccgcgttt gctggagctg ggccatcaaa tcacggtagt gacgcgtaac ccacagaaag
    32341 ccagttccgt tctcggccct cgggtgacgc tatggcaagg gctagccgat caaagcaacc
    32401 tcaacggcgt tgatgcggta atcaacctgg ccggagaacc gattgctgat aaacgctgga
    32461 ctcacgagca aaaagagcgt ctctgccaaa gccgctggaa tatcacgcaa aaactggtcg
    32521 atttgattaa tgccagcgac acgccgccgt cggtattcat ttccggctcg gcaacgggct
    32581 attatggcga cttaggtgaa gtggtggtta ccgaagagga accgccgcat aacgaattta
    32641 ctcataaact ctgcgcccgc tgggaagaga ttgcctgccg ggcgcaaagt gacaaaacgc
    32701 gagtgtgcct gctgcgaacc ggcgtggtac tggcaccgga cggcggtatt ctcggtaaaa
    32761 tgctgccacc gtttcgtctt ggcctgggcg ggccgattgg ttccggtcgg cagtatctgg
    32821 cctggattca tattgatgat atggtcaacg gcattctctg gctgctggat aacgacctgc
    32881 gcgggccatt taatatggtt tcgccctacc cggtacgcaa tgaacaattt gcccatgcgc
    32941 tcggtcaagc gctgcatcgc ccggccattt tgcgcgtccc cgcaaccgcc attcggctgt
    33001 taatgggcga atcttcaata ctggtattag gtggacaacg cgcgctgcct aaacggctgg
    33061 aagaagcggg ttttgcgttt cgctggtacg atttagaaga ggcgctggcg gatgtcgttc
    33121 gctgatgtgg tttacagcaa acatccgcca gttaactccc ggtgttacag gattagtggc
    33181 tttgcgcgat aagatcgtct ggtgaaagtc gggtcaccat cataactaac tctctgtcta
    33241 aacctctatc cagcatctcc tgagcaatac gcagggcttc ttcgtgtttg ccctgcattg
    33301 cgccttcttc acgtaatctg tcagcaatgg tcatcagttt ctccttttct tgtggtgcgc
    33361 gttctgttat ctcaccaata aatgcacgga aacgctgggc atcccctgtt tgtaatacgt
    33421 aattaaacag ggcttttagc tgtctgtcat tagtgttccc tgtaactagc agcgaaacaa
    33481 tttggtcgac taatcccaac agatcgcgct gacgaatatg tttctgaatt aactccaaca
    33541 gcgccatttt gcggtgttgc ataatctcgt catccggcac cacggtaata tccaccaacg
    33601 gaaacgctga tgaatatatt ttgcgggcta tagcaggctc ggcaaattca tcaagccagc
    33661 agagtgaata aggataagga cttctgcaac catgataaaa cagcatcggg atcaccaatg
    33721 gaagctcttt atagcccgca tcaagatggt tttgcattgc cgcaatggaa taacgcatca
    33781 tgcgaaaagc cattaattct tccagcttac tttggtgctc tatcactaca taaatataac
    33841 ccactccctc ctgcgttttc acagaccaca agaggtcgga ataatattgc cgcaggtctt
    33901 catcaataaa actgtttgat tctagtttaa gcgtcgttaa atcacacagt ttgcgcagcg
    33961 gcgcgggaag atgaatatca ataaaatccc gcgcggtgtc t
//
LOCUS       DADMKF010000030        33440 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.030, whole genome shotgun sequence.
ACCESSION   DADMKF010000030 DADMKF010000000
VERSION     DADMKF010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33440
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.030"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            521..760
                     /locus_tag="JNC24_001300"
     CDS             521..760
                     /locus_tag="JNC24_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001353740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1819 family protein"
                     /protein_id="HAY9158097.1"
                     /translation="MTPFRYNSDLTSGSLQTRECRIITGLLLQELDEAAWDKAMYKEN
                     VLQKRTQSTVRRISSALRKRLEHLSSDFWAFAFLC"
     gene            complement(948..1925)
                     /gene="intI2"
                     /locus_tag="JNC24_001301"
                     /pseudo
     CDS             complement(948..1925)
                     /gene="intI2"
                     /locus_tag="JNC24_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_023994230.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class 2 integron integrase IntI2"
     gene            2257..2730
                     /locus_tag="JNC24_001302"
     CDS             2257..2730
                     /locus_tag="JNC24_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HAY9158098.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            2825..3349
                     /gene="sat2"
                     /locus_tag="JNC24_001303"
     CDS             2825..3349
                     /gene="sat2"
                     /locus_tag="JNC24_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat2"
                     /protein_id="HAY9158099.1"
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSP
                     YRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAH
                     SLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNET
                     AMYWYWFSGAQDDA"
     gene            3407..4195
                     /gene="aadA1"
                     /locus_tag="JNC24_001304"
     CDS             3407..4195
                     /gene="aadA1"
                     /locus_tag="JNC24_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HAY9158100.1"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
     gene            4271..4768
                     /locus_tag="JNC24_001305"
     CDS             4271..4768
                     /locus_tag="JNC24_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9158101.1"
                     /translation="MRDLLKVVLSLVLAMASGQALADLPIVLVDEARLPYDYSPSNYD
                     ISPSNYDNSISNYDNSPSNYDNSESNYDNSSSNYDNSRNGNRRLIYSANGSRTFAGYY
                     VIANNGTTNFFSTSGKRMFYTPKGGRGVYGGKDGSFCGALVVINGQFSLALTDNGLKI
                     MYLSN"
     gene            4829..5200
                     /locus_tag="JNC24_001306"
     CDS             4829..5200
                     /locus_tag="JNC24_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9158102.1"
                     /translation="MSYEPPRDGYTNGYIVDVMYDNRPSKYPAVFRTYVRIPDYVELD
                     NAEELADAIIREVELKEAWLTRVLGNSADPDASVGKWDRRLAQCGGIDEMSSGVQATY
                     FSPNGQLVGLVAYIDAEWMDE"
     gene            5610..5987
                     /locus_tag="JNC24_001307"
     CDS             5610..5987
                     /locus_tag="JNC24_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9158103.1"
                     /translation="MSAAKALAYALCDKLESKKVDEVVRNLVSSGWNIREVAWEASSF
                     DHEMPYFDTGRDFDIGFPDTLSSKIEFLTANGSIVQARARLHFKQALIFAKASKHVAA
                     LKNSLDYYYGSSVIPPQNHGGYL"
     gene            6010..6162
                     /locus_tag="JNC24_001308"
                     /pseudo
     CDS             6010..6162
                     /locus_tag="JNC24_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011069623.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(6218..7834)
                     /locus_tag="JNC24_001309"
     CDS             complement(6218..7834)
                     /locus_tag="JNC24_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000251878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator"
                     /protein_id="HAY9158104.1"
                     /translation="MVRLATFNDNVQVVHIGHLFRNSGHKEWRIFVWFNPMQERKWTR
                     FTHLPLLSRAKVVNSTTKQINKADRVIEFEASDLQRAKIIDFPNLSSFASVRNKDGAQ
                     SSFIYEAETPYSKTRYHIPQLELARSLFLINSYFCRSCLSSTALQQEFDVQYEVERDH
                     LEIRILPSSSFPKGALEQSAVVQLLVWLFSDQDVMDSYESIFRHYQQNREIKNGVESW
                     CFSFDPPPMQGWKLHVKGRSSNEDKDYLVEEIVGLEINAMLPSTTAISHASFQEKEAG
                     DGSTQHIAVSTESVVDDEHLQLDDEETANIDTDTRVIEAEPTWISFSRPSRIEKSRRA
                     RKSSQTILEKEEATTSENSNLVSTDEPHLGGVLAAADVGGKQDATNYNSIFANRFAAF
                     DELLSILKTKFACRVLFEETLVLPKVGRSRLHLCKDGSPRVIKAVGVQRNGSEFVLLE
                     VDASDGVKMLSTKVLSGVDSETWRNDFEKIRRGVVKSSLNWPNSLFDQLYGQDGHRGV
                     NHPKGLGELQVSREDMEGWAERVVREQFTH"
     gene            complement(7835..9361)
                     /locus_tag="JNC24_001310"
     CDS             complement(7835..9361)
                     /locus_tag="JNC24_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator"
                     /protein_id="HAY9158105.1"
                     /translation="MRNFPVPYSNELIYSTIARAGVYQGIVSPKQLLDEVYGNRKVVA
                     TLGLPSHLGVIARHLHQTGRYAVQQLIYEHTLFPLYAPFVGKERRDEAIRLMEYQAQG
                     AVHLMLGVAASRVKSDNRFRYCPDCVALQLNRYGEAFWQRDWYLPALPYCPKHGALVF
                     FDRAVDDHRHQFWALGHTELLSDYPKDSLSQLTALAAYIAPLLDAPRAQELSPSLEQW
                     TLFYQRLAQDLGLTKSKHIRHDLVAERVRQTFSDEALEKLDLKLAENKDTCWLKSIFR
                     KHRKAFSYLQHSIVWQALLPKLTVIEALQQASALTEHSITTRPVSQSVQPNSEDLSVK
                     HKDWQQLVHKYQGIKAARQSLEGGVLYAWLYRHDRDWLVHWNQQHQQERLAPAPRVDW
                     NQRDRIAVRQLLRIIKRLDSSLDHPRATSSWLLKQTPNGTSLAKNLQKLPLVALCLKR
                     YSESVEDYQIRRISQAFIKLKQEDVELRRWRLLRSATLSKERITEEAQRFLEMVYGEE
                     "
     gene            complement(9364..11031)
                     /locus_tag="JNC24_001311"
     CDS             complement(9364..11031)
                     /locus_tag="JNC24_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAY9158106.1"
                     /translation="MSATRIQAVYRDTGVEAYRDNPFIEALPPLQESVNSAASLKSSL
                     QLTSSDLQKSRVIRAHTICRIPDDYFQPLGTHLLLSERISVMIRGGYVGRNPKTGDLQ
                     KHLQNGYERVQTGELETFRFEEARSTAQSLLLIGCSGSGKTTSLHRILATYPQVIYHR
                     ELNVEQVVYLKIDCSHNGSLKEICLNFFRALDRALGSNYERRYGLKRHGIETMLALMS
                     QIANAHALGLLVIDEIQHLSRSRSGGSQEMLNFFVTMVNIIGVPVMLIGTPKAREIFE
                     ADLRSARRGAGFGAIFWDPIQQTQRGKPNQEWIAFTDNLWQLQLLQRKDALLSDEVRD
                     VWYELSQGVMDIVVKLFVLAQLRALALGNERITAGLLRQVYQDELKPVHPMLEALRSG
                     IPERIARYSDLVVPEIDKRLIQLQLDIAAIQEQTPEEKALQELDTEDQRHLYLMLKED
                     YDSSLLIPTIKKAFSQNPTMTRQKLLPLVLQWLMEGETVVSELEKPSKSKKVSAIKVV
                     KPSDWDSLPDTDLRYIYSQRQPEKTMHERLKGKGVIVDMASLFKQAG"
     gene            complement(11028..13136)
                     /locus_tag="JNC24_001312"
     CDS             complement(11028..13136)
                     /locus_tag="JNC24_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000267723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HAY9158107.1"
                     /translation="MWQINEVVLFDNDPYRILAIEDGQVVWMQISADKGVPQARAELL
                     LMQYLDEGRLVRTDDPYVHLDLEEPSVDSVSFQKREEDYRKILPIINSKDRFDPKVRS
                     ELVEHVVQEHKVTKATVYKLLRRYWQRGQTPNALIPDYKNSGAPGERRSATGTAKIGR
                     AREYGKGEGTKVTPEIERLFRLTIEKHLLNQKGTKTTVAYRRFVDLFAQYFPRIPQED
                     YPTLRQFRYFYDREYPKAQRLKSRVKAGVYKKDVRPLSSTATSQALGPGSRYEIDATI
                     ADIYLVDHHDRQKIIGRPTLYIVIDVFSRMITGFYIGFENPSYVVAMQAFVNACSDKT
                     AICAQHDIEISSSDWPCVGLPDVLLADRGELMSHQVEALVSSFNVRVESAPPRRGDAK
                     GIVESTFRTLQAEFKSFAPGIVEGSRIKSHGETDYRLDASLSVFEFTQIILRTILFRN
                     NHLVMDKYDRDADFPTDLPSIPVQLWQWGMQHRTGSLRAVEQEQLRVALLPRRKVSIS
                     SFGVNLWGLYYSGSEILREGWLQRSTDIARPQHLEAAYDPVLVDTIYLFPQVGSRVFW
                     RCNLTERSRQFKGLSFWEVWDIQAQEKHNKANAKQDELTKRRELEAFIQQTIQKANKL
                     TPSTTEPKSTRIKQIKTNKKEAVTSERKKRAEHLKPSSSGDEAKVIPFNAVEADDQED
                     YSLPTYVPELFQDPPEKDES"
     gene            complement(13123..13944)
                     /locus_tag="JNC24_001313"
     CDS             complement(13123..13944)
                     /locus_tag="JNC24_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heteromeric transposase endonuclease subunit
                     TnsA"
                     /protein_id="HAY9158108.1"
                     /translation="MAKANSSFSEVQIARRIKEGRGQGHGKDYIPWLTVQEVPSSGRS
                     HRIYSHKTGRVHHLLSDLELAVFLSLEWESSVLDIREQFPLLPSDTRQIAIDSGIKHP
                     VIRGVDQVMSTDFLVDCKDGPFEQFAIQVKPAAALQDERTLEKLELERRYWQQKQIPW
                     FIFTDKEINPVVKENIEWLYSVKTEEVSAELLAQLSPLAHILQEKGDENIINVCKQVD
                     IAYDLELGKTLSEIRALTANGFIKFNIYKSFRANKCADLCISQVVNMEELRYVAN"
     gene            complement(14103..15932)
                     /gene="glmS"
                     /locus_tag="JNC24_001314"
     CDS             complement(14103..15932)
                     /gene="glmS"
                     /locus_tag="JNC24_001314"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HAY9158109.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLDGGTGIAHTRWATHGEPSEANAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            complement(16094..17464)
                     /gene="glmU"
                     /locus_tag="JNC24_001315"
     CDS             complement(16094..17464)
                     /gene="glmU"
                     /locus_tag="JNC24_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HAY9158110.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGSKVGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            complement(17815..18234)
                     /gene="atpC"
                     /locus_tag="JNC24_001316"
     CDS             complement(17815..18234)
                     /gene="atpC"
                     /locus_tag="JNC24_001316"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HAY9158111.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            complement(18255..19637)
                     /gene="atpD"
                     /locus_tag="JNC24_001317"
     CDS             complement(18255..19637)
                     /gene="atpD"
                     /locus_tag="JNC24_001317"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HAY9158112.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            complement(19664..20527)
                     /gene="atpG"
                     /locus_tag="JNC24_001318"
     CDS             complement(19664..20527)
                     /gene="atpG"
                     /locus_tag="JNC24_001318"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HAY9158113.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKSVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            complement(20578..22119)
                     /gene="atpA"
                     /locus_tag="JNC24_001319"
     CDS             complement(20578..22119)
                     /gene="atpA"
                     /locus_tag="JNC24_001319"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HAY9158114.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            complement(22132..22665)
                     /gene="atpH"
                     /locus_tag="JNC24_001320"
     CDS             complement(22132..22665)
                     /gene="atpH"
                     /locus_tag="JNC24_001320"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HAY9158115.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            complement(22680..23150)
                     /gene="atpF"
                     /locus_tag="JNC24_001321"
     CDS             complement(22680..23150)
                     /gene="atpF"
                     /locus_tag="JNC24_001321"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HAY9158116.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            complement(23212..23451)
                     /gene="atpE"
                     /locus_tag="JNC24_001322"
     CDS             complement(23212..23451)
                     /gene="atpE"
                     /locus_tag="JNC24_001322"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HAY9158117.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            complement(23498..24313)
                     /gene="atpB"
                     /locus_tag="JNC24_001323"
     CDS             complement(23498..24313)
                     /gene="atpB"
                     /locus_tag="JNC24_001323"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HAY9158118.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYSIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            complement(24322..24702)
                     /gene="atpI"
                     /locus_tag="JNC24_001324"
     CDS             complement(24322..24702)
                     /gene="atpI"
                     /locus_tag="JNC24_001324"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HAY9158119.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAYTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            complement(25319..25942)
                     /gene="rsmG"
                     /locus_tag="JNC24_001325"
     CDS             complement(25319..25942)
                     /gene="rsmG"
                     /locus_tag="JNC24_001325"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HAY9158120.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            complement(26006..27895)
                     /gene="mnmG"
                     /locus_tag="JNC24_001326"
     CDS             complement(26006..27895)
                     /gene="mnmG"
                     /locus_tag="JNC24_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HAY9158121.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     LYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASQHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLVGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            complement(28274..28717)
                     /gene="mioC"
                     /locus_tag="JNC24_001327"
     CDS             complement(28274..28717)
                     /gene="mioC"
                     /locus_tag="JNC24_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HAY9158122.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     SASGIWLIISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            complement(28807..29265)
                     /gene="asnC"
                     /locus_tag="JNC24_001328"
     CDS             complement(28807..29265)
                     /gene="asnC"
                     /locus_tag="JNC24_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HAY9158123.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLAEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            29417..30409
                     /gene="asnA"
                     /locus_tag="JNC24_001329"
     CDS             29417..30409
                     /gene="asnA"
                     /locus_tag="JNC24_001329"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HAY9158124.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVSEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRENVPSLL"
     gene            complement(30414..31865)
                     /gene="viaA"
                     /locus_tag="JNC24_001330"
     CDS             complement(30414..31865)
                     /gene="viaA"
                     /locus_tag="JNC24_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HAY9158125.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQLIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVIERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(31859..33355)
                     /gene="ravA"
                     /locus_tag="JNC24_001331"
     CDS             complement(31859..33355)
                     /gene="ravA"
                     /locus_tag="JNC24_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HAY9158126.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRLQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTVIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSAQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEASLQDVGAQIHQAQQC"
BASE COUNT         8519 a         8451 c         7794 g         8676 t
ORIGIN      
        1 tcggcagccc ttgtgggcgg acaaaatagt tgggaactgg gaggggtgga aatggagttt
       61 ttaaggatta tttagggaag agtgacaaaa tagatgggaa ctgggtgtag cgtcgtaagc
      121 taatacgaaa attaaaaatg acaaaatagt ttggaactag atttcactta tctggttaac
      181 taatctagct aacctgccgc tcagcttagt acgatcctat atccaactga tcataatcct
      241 ttcatcagta tcccaaatta aatccccctc ctagactgcc tcaccaacac tttacagtga
      301 tcccgtattt ccggacaggt gattgatttt tttcgcttgt acaggtgtta agccacagtt
      361 atcttgatgt ggacattatg cattgtaata gcccattaac atgcgctaag agcataaatt
      421 aatatacagt aacaagcatt acgaggtgct aatgtttgga aagcagcctc ttccaactac
      481 aataataagt taactatcct ccacccacaa ataattacaa atgacccctt tccgctataa
      541 ctccgatctc accagcggct cgctacaaac ccgagagtgt cgcattatta ctggcttgtt
      601 actccaagag cttgatgaag ctgcctggga taaagccatg tataaggaaa atgtgcttca
      661 gaaacgcacg caatctacgg taagacgtat ttcttcagcg cttagaaaac gcctagaaca
      721 cctaagctct gacttttggg cttttgcgtt tttatgttag gcattgtctg gcaactggag
      781 gaagtgtaat gaccatgaat ggtcactgtg attgacagta tcttgcttaa acttatctga
      841 acaatggtag aactggctca tatttatccc ctctatgatt gtttatcttt ttcaccataa
      901 aaagtatcag gggctactca gggctacata aagatattta tctactttta ctgattgata
      961 agtagcatca gtccatccgc aggactggtg gtgccggcaa aatgctgacc caacacatgc
     1021 gtatagattt gcgtggtctt aacatcgtta tgccctaaga gttcttgcac agtgcgaata
     1081 tcacgccccg cttgtaatag atgcgtagca aacgagtgac gaaatgtatg acaagtgaca
     1141 cgcttgctaa cgatgcctgc tttttgtacg gctgccttca atgcctttcg cgcaacggag
     1201 tcatgcagat gatggcggca taatttgccg ttatacgggt ggttgcagag cgtgctggag
     1261 ggaaagacaa acatccacgc cgcttgtcga taagcagaag ggtatttgtg atctaaagca
     1321 aaaggcagcg atggccctac gccttgtaag ttgtcgtctt gctgaataag ccgcgcttgc
     1381 tcaatgagtt attttattgc tgggattagg cgcgtgggca gtaggctgtt tctgcttttc
     1441 ccacccttac cgtcatgcac agtgatgcag ccattatcaa aatcaaaatc tttaacccgc
     1501 aaacgcaagc attcattaat gcgcaaacct gcaccataca gcagcgtaaa aataacttgg
     1561 ttgcgagtat ccataacctg caaaatgcgt tgcacttcat ttgcagagat aacagagggt
     1621 agccgtctag gcttgcttgc agggatataa tcaatatcgc ccaacggctg ttgtaaaaac
     1681 ctgttgtaca aaaaagctag ggcatttaaa gcgattttct gcgtgtttat ggctacatgt
     1741 ctgctgtttg ctaagctgga taaaaacagc ctgacctctt cactgcccat ggtctgagga
     1801 tgacgttttt tgtgaaacag aataaaacgc ttaatccagt gcaggtaagt tttttcagtt
     1861 ttcagcgcat aacctttttg tcgcatatcc gtgcgtatag aatttaaaaa tggactgtta
     1921 gacataaaac gctccttgtc ttgcaactgt ctgcctatac agcctattct agctgggatt
     1981 taaaaaagtg cctgtttttt acgcctagag atgcttgttt accggtagag ttttaattta
     2041 atgctaaata aattaaaatg ttatgagttc tttgggtgag ataatgtgca tcgtgcaagc
     2101 aggatagacg gcatgcacga tttgtaataa cagagtgtct tgtattttta aagaaagtct
     2161 atttaataca agtgattata ttaattaacg gtaagcatca gcgggtgaca aaacgagcat
     2221 gcttactaat aaaatgttaa cctctgagga agaattgtga aactatcact aatggtagct
     2281 atatcgaaga atggagttat cgggaatggc cctgatattc catggagtgc caaaggtgaa
     2341 cagctcctgt ttaaagctat tacctataac caatggctgt tggttggacg caagactttt
     2401 gaatcaatgg gagcattacc caaccgaaag tatgcggtcg taacacgttc aagttttaca
     2461 tctgacaatg agaacgtatt gatctttcca tcaattaaag atgctttaac caacctaaag
     2521 aaaataacgg atcatgtcat tgtttcaggt ggtggggaga tatacaaaag cctgatcgat
     2581 caagtagata cactacatat atctacaata gacatcgagc cggaaggtga tgtttacttt
     2641 cctgaaatcc ccagcaattt taggccagtt tttacccaag acttcgcctc taacataaat
     2701 tatagttacc aaatctggca aaagggttaa caagtggcag caacggattc gcaaacctgt
     2761 cacgcctttt gtaccaaaag ccgcgccagg tttgcgatcc gctgtgccag gcgttaggcg
     2821 tcatatgaag atttcggtga tccctgagca ggtggcggaa acattggatg ctgagaacca
     2881 tttcattgtt cgtgaagtgt tcgatgtgca cctatccgac caaggctttg aactatctac
     2941 cagaagtgtg agcccctacc ggaaggatta catctcggat gatgactctg atgaagactc
     3001 tgcttgctat ggcgcattca tcgaccaaga gcttgtcggg aagattgaac tcaactcaac
     3061 atggaacgat ctagcctcta tcgaacacat tgttgtgtcg cacacgcacc gaggcaaagg
     3121 agtcgcgcac agtctcatcg aatttgcgaa aaagtgggca ctaagcagac agctccttgg
     3181 catacgatta gagacacaaa cgaacaatgt acctgcctgc aatttgtacg caaaatgtgg
     3241 ctttactctc ggcggcattg acctgttcac gtataaaact agacctcaag tctcgaacga
     3301 aacagcgatg tactggtact ggttctcggg agcacaggat gacgcctaac aattcattca
     3361 agccgacacc gcttcgcggc gcggcttaat tcaggagtta aacatcatga gggaagcggt
     3421 gatcgccgaa gtatcgactc aactatcaga ggtagttggc gtcatcgagc gccatctcga
     3481 accgacgttg ctggccgtac atttgtacgg ctccgcagtg gatggcggcc tgaagccaca
     3541 cagtgatatt gatttgctgg ttacggtgac cgtaaggctt gatgaaacaa cgcggcgagc
     3601 tttgatcaac gaccttttgg aaacttcggc ttcccctgga gagagcgaga ttctccgcgc
     3661 tgtagaagtc accattgttg tgcacgacga catcattccg tggcgttatc cagctaagcg
     3721 cgaactgcaa tttggagaat ggcagcgcaa tgacattctt gcaggtatct tcgagccagc
     3781 cacgatcgac attgatctgg ctatcttgct gacaaaagca agagaacata gcgttgcctt
     3841 ggtaggtcca gcggcggagg aactctttga tccggttcct gaacaggatc tatttgaggc
     3901 gctaaatgaa accttaacgc tatggaactc gccgcccgac tgggctggcg atgagcgaaa
     3961 tgtagtgctt acgttgtccc gcatttggta cagcgcagta accggcaaaa tcgcgccgaa
     4021 ggatgtcgct gccgactggg caatggagcg cctgccggcc cagtatcagc ccgtcatact
     4081 tgaagctagg caggcttatc ttggacaaga agatcgcttg gcctcgcgcg cagatcagtt
     4141 ggaagaattt gttcactacg tgaaaggcga gatcaccaag gtagtcggca aataatgtct
     4201 aacaattcgt tcaagccgac gccgcttcgc ggcgcggctt aactcaagcg ttagagagct
     4261 ggggaagact atgcgcgatc tgttgaaggt ggttctaagc ctcgtacttg cgatggcatc
     4321 ggggcaggca cttgctgacc tgccaattgt tttagtggat gaagctcgtc ttccctatga
     4381 ctactcccca tccaactacg acatttctcc aagcaactac gacaactcca taagcaatta
     4441 cgacaatagt ccatcaaatt acgacaactc tgagagcaac tacgataata gttcatccaa
     4501 ttacgacaat agtcgcaacg gaaatcgtag gcttatatat agcgcaaatg ggtctcgcac
     4561 tttcgccggc tactacgtca ttgccaacaa tgggacaacg aacttctttt ccacatctgg
     4621 caaaaggatg ttctacaccc caaaaggggg gcgcggcgtc tatggcggca aagatgggag
     4681 cttctgcggg gcattggtcg tcataaatgg ccaattttcg cttgccctga cagataacgg
     4741 cctgaagatc atgtatctaa gcaactagcc tgctctctaa taaaatgtta ggcctcaaca
     4801 tctagtcgca agctgagggg aaccactagt gtcatacgaa cctccaagag acggttacac
     4861 aaacgggtac attgttgatg tcatgtatga caatcgccca agtaagtatc cagctgtgtt
     4921 cagaacgtac gtccgaattc cagactatgt cgaactagat aacgcggagg agctggccga
     4981 cgcaatcatt cgagaggttg agttaaagga ggcatggctc acaagggtct tggggaactc
     5041 agccgatcct gacgcttctg tgggcaagtg ggaccggcgc ctagcgcagt gcggtggtat
     5101 tgatgagatg tcaagtgggg tgcaggcaac atacttcagt ccaaacggtc agctggtcgg
     5161 cttagtggca tacattgatg ccgaatggat ggatgaatag ctctgacgtt acgattcagg
     5221 tgagacaacc agatgggcat tgcgtacaag ctcagttgcc accgatgtgg ctacgagtct
     5281 gatcttcttt acctaggcca aggtatggcg atgcttcctg agcaaatagt cggaacgtgc
     5341 acatgctgcg acaatctaac gacgatttct ataaatgaaa gctacggagt atgctcactt
     5401 tgtggggcta aggatcgtgt gatatttaca agctatcagc atgaggcaag gccaagaaat
     5461 ccctgctatc tagagagaag tttcaaatat caatgcccaa gatgccatgc cttcagtatg
     5521 gagcctccgg atctaccgga gatgctttgg gactaatgct ccgctttgcc tcgggtatcc
     5581 gttcgaccta tgtttaagag gaaacgcacg tgtctgccgc caaagctctt gcctatgcac
     5641 tttgtgacaa gttggaaagc aaaaaagttg atgaagtcgt acgaaatctt gtttctagcg
     5701 gctggaatat tagggaagtg gcctgggaag cttcgtcctt tgatcatgaa atgccctact
     5761 ttgacacggg gcgggacttc gatattggat ttcctgacac gctgtcatcc aagattgagt
     5821 ttttgaccgc gaatggatca atcgtccaag cacgtgctcg cctgcacttc aagcaggctc
     5881 taatttttgc caaggcgtcc aaacatgtcg ccgcgctcaa gaactcattg gattactact
     5941 atggatcgtc tgtaatcccc cctcaaaacc acggaggcta tttgtagaaa attccattaa
     6001 tatgaactca aggtgttccc tacggacgaa tcagcgatga aggtggtgta cctggctatc
     6061 caggcggcga ccaagaaatg gacgatgccg atccgcaact ggaagccggc catgaaccgt
     6121 tttatgatcg aatttggtga ccgtttaaat ggccaccttt aactgaccgg caaaagcact
     6181 tacacaaaag ggtttacagg ctctttcagt cattccttta atgcgtaaat tgctctctaa
     6241 ccactctctc agcccaccct tccatatcct ctctcgatac ttgcagctcc cccaacccct
     6301 ttggatgatt cacccctcta tgcccgtctt gtccatataa ttgatcaaac aaactatttg
     6361 gccaattcaa tgagctcttc accactccac gccgtatctt ttcaaaatca ttccgccatg
     6421 tttcgctatc aacgccactc aacactttgg tagaaagcat tttcacccca tccgatgcat
     6481 ccacctctag caatacaaat tcactgccat tacgttgcac cccaacggct ttaatcactc
     6541 ttggtgagcc atctttacac agatgtaatc ggctacgccc aacttttggc aaaaccaagg
     6601 tttcttcaaa aagcacccga catgcaaatt tagtttttag aattgaaagt agctcatcaa
     6661 aagcagcaaa tcgattagca aaaatagagt tgtaattggt tgcatcctgc ttcccaccca
     6721 catctgccgc tgctaggaca ccacctaagt gtggctcatc agtactaacc aaattactat
     6781 tttcacttgt tgttgcttct tctttttcta aaatagtttg gctacttttt cttgccctgc
     6841 gagatttttc aattcgacta ggtctactaa aacttatcca tgtcggctca gcctctatga
     6901 ctcgtgtgtc tgtgtctata ttggctgttt cctcatcgtc caactgtaga tgctcatcat
     6961 caacaactga ctctgttgaa accgctatgt gctgcgtact accatcacct gcctcctttt
     7021 cctgaaaaga ggcatggcta atagctgttg tgctaggaag catagcgttg atttctaaac
     7081 ctactatttc ctcaactaaa taatccttat cctcgttaga agaacgtcct tttacatgta
     7141 atttccaacc ctgcatgggc ggagggtcaa agctaaagca ccagctttca acgccgttct
     7201 taatttctct attttgttga tagtgcctaa aaatactttc atacgaatcc ataacatctt
     7261 gatccgaaaa caaccaaacc aaaagctgca ctacggccga ctgctctaac gcccctttag
     7321 gaaacgatga actgggtaag atccttatct ctaaatgatc tcgctcaacc tcatactgaa
     7381 cgtcgaactc ttgctgtaaa gcggtactgc tcaaacagct tcgacagaaa taggagttaa
     7441 ttaaaaataa tgaccgagct agctctaact gtgggatgtg ataacgagtc ttgctatatg
     7501 gtgtttcagc ttcgtaaata aatgaactct gcgctccatc cttgttgcgt acggaagcaa
     7561 aggacgagag attaggaaaa tcgattattt tggctcgttg aagatccgat gcttcaaact
     7621 caatcacacg atccgcctta tttatttgct ttgttgtact gttaaccacc ttagctcgac
     7681 ttaataaagg caaatgagta aatcgagtcc atttccgttc ttgcattgga ttaaaccaaa
     7741 caaaaatacg ccactcctta tgacccgagt tacggaataa atgaccaata tgtacaacct
     7801 gcacattgtc attaaatgta gctagcctaa ccactcactc ttccccataa accatttcca
     7861 agaatctttg tgcttcctca gttatccgct ctttagataa cgttgcactt cttaataatc
     7921 gccagcgcct aagctcaaca tcttcctgtt taagcttaat aaaagcttgg ctaatccgtc
     7981 taatttgata atcttccaca ctctctgagt aacgctttaa gcaaagcgct accaaaggca
     8041 gtttctgtag attttttgca agagaggttc cgttaggagt ttgctttaac agccagctcg
     8101 atgtcgctct tgggtgatca aggctactat ctagacgctt tatgattctt aatagttgtc
     8161 gtacagcaat tcgatctctt tggttccaat caactctagg ggcgggtgcc agacgctctt
     8221 gttgatgctg ttgattccag tgaactagcc aatccctgtc atgtcggtaa agccaagcgt
     8281 atagcacccc accctctaaa gactgtcttg ccgccttaat tccttggtat ttatgcacta
     8341 gttgctgcca gtctttatgc ttaacagata aatcttcaga gttaggttgc acagactggc
     8401 taacaggtct tgtcgttata gagtgctcag taagagcact tgcctgctgt agcgcttcta
     8461 taaccgttag ttttggcaat aaggcttgcc acacaatact atgctgtaaa taactaaagg
     8521 cttttctatg cttacggaat atacttttca gccaacacgt gtccttgttc tctgccaact
     8581 ttaaatccag tttctctagt gcctcatcac taaaagtctg cctcactctc tccgccacca
     8641 agtcatgacg aatgtgcttg cttttggtta gccctagatc ctgcgctaag cgctgataaa
     8701 acagcgtcca ctgctcaagg cttggggaaa gctcttgcgc tcgtggagca tctaacagag
     8761 gggctatata agcagctagt gctgttaatt gagataggga gtctttgggg tagtctgaaa
     8821 gcagctcagt atgacccaaa gcccaaaatt gatgtcggtg atcatctaca gctctatcaa
     8881 agaagactaa agcaccgtgt tttggacaat atggcaaagc gggcaaatac caatctcgtt
     8941 gccaaaaggc ttccccatac ctatttagct gaagagcaac gcaatcaggg cagtatctaa
     9001 agcggttatc gctcttaact ctagaagcag cgactcctag cattaaatgc accgcacctt
     9061 gcgcttggta ctccattaac cgaatagctt cgtctcggcg ctccttgcct acaaacggag
     9121 catataaagg gaataaggta tgctcataaa taagctgctg aacagcgtaa cgtcctgttt
     9181 gatgtagatg tcttgctatc acacctaaat gcgagggcag acctaaggta gcgaccacct
     9241 tgcggttgcc atacacctca tccaacagct gcttaggact aacaatccct tgataaacgc
     9301 ctgcccgtgc aatagtgcta taaatcagct cattcgagta cggaacagga aagtttctca
     9361 tggctaacct gcttgtttaa ataagctcgc catatccact attacccctt tcccttttaa
     9421 ccgttcatgc atggtttttt caggttggcg ttgtgaatag atataacgta aatccgtatc
     9481 aggcaagcta tcccagtcgc tgggcttgac tacctttata gccgaaacct ttttactctt
     9541 ggagggcttt tctagttctg acactaccgt ttcgccttcc atcaaccact gcaaaacaag
     9601 aggcagtaac ttttgtcttg tcatcgttgg attctggcta aacgcttttt taatagtggg
     9661 aattaacagg cttgaatcgt aatcctcttt cagcatcaga tataaatgac gctgatcttc
     9721 ggtatctaac tcttgaaggg ctttttcttc tggtgtttgt tcttgtatcg ctgcgatatc
     9781 tagctgaagt tggattaacc gtttatcaat ctcgggaacg actagatcag aataacgagc
     9841 aatgcgttct gggatacccg agcgtaatgc ctctagcatg gggtgcacag gctttaactc
     9901 atcttgatac acttgccgca ataaaccagc ggtaatacgc tcattgccta aagctagcgc
     9961 acggagctga gcgagtacaa aaagttttac tacaatgtcc atcactcctt ggcttagctc
    10021 ataccacaca tcacggacct catccgataa cagcgcatct ttgcgttgta aaagctgtaa
    10081 ttgccaaaga ttatccgtaa aagcgatcca ctcttgattg ggctttccac gttgcgtttg
    10141 ttgtatagga tcccagaata tagctccaaa ccctgcccct ctacgtgcag accgcaaatc
    10201 agcctcaaaa atctctcgtg ctttaggggt accaatcaac atcactggta cgccaataat
    10261 attcaccatc gtcacaaaaa agttcagcat ctcttgagat ccacccgaac gagagcggct
    10321 taaatgctga atttcatcaa taaccaacaa ccctaaagca tgtgcattgg ctatttgcga
    10381 catcaaagcc aacatggttt ctataccatg acgttttaag ccataacgac gctcatagtt
    10441 cgagcccaag gctcgatcca acgctctgaa aaaattcaag cagatttctt ttagcgaacc
    10501 attatgcgag cagtctattt tcaaatacac cacctgctct acattgagtt cacgatggta
    10561 aatcacctga ggatacgtgg ctagaatacg atgaagagag gtcgtcttcc cactaccaga
    10621 acaaccaatt aacaataagc tttgtgccgt agatcgtgcc tcctcaaagc gaaatgtctc
    10681 caactctccc gtttgaacac gctcataacc attttgtaaa tgcttttgta aatctcctgt
    10741 tttaggattt ctgcctacgt agccacctcg aatcatgacc gaaatacgct cacttagtag
    10801 caaatgcgta cctaatggct gaaaatagtc atctggaata cgacaaatgg tatgagctct
    10861 gataacacgg gacttttgca agtcagagga agtaagctgt aaagaggatt tcagtgatgc
    10921 agcactattc actgactctt gtaatggtgg taaggcctcg ataaaaggat tatcacgata
    10981 agcctctacc cccgtatcac gataaactgc ttgaatccgg gtagcactca tgactcatcc
    11041 ttttctggtg gatcctgaaa taattcaggc acgtatgtgg gtaggctgta atcttcttga
    11101 tcatccgctt ccactgcgtt gaaaggaata actttagcct catcacctga agagcttggc
    11161 ttcaaatgct ccgcacgttt tttacgctcc gaggtcacgg cttctttttt attagtttta
    11221 atctgcttaa tgcgtgttga tttgggctca gtagtactgg gcgttaactt attcgctttc
    11281 tgaatggttt gctgaataaa cgcctcaagc tccctgcgtt tagttaactc atcctgcttc
    11341 gcattggctt tattgtgttt ttcttgtgct tgtatatccc aaacctccca aaatgagaga
    11401 cctttaaact gccgactacg ttccgtcaga ttacagcgcc aaaatacacg gctgccaact
    11461 tgcggaaaca aataaatcgt atcaaccagc actgggtcat aagccgcttc taaatgttga
    11521 ggtctagcta tatcagtgct ccgctgcaac caaccctcac gcagaatctc tgaccccgag
    11581 taatacaaac cccacaaatt aacgccaaat gaagaaatag agacctttcg gcgaggcagt
    11641 aacgctactc gcaactgctc ttgctccaca gcccttaaac tacctgtacg atgctgcata
    11701 ccccattgcc atagctggac aggaatagac ggtaaatctg taggaaaatc agcatctcga
    11761 tcgtatttat ccatcaccag atggttattt ctgaataaga tcgtacgcaa aataatttgt
    11821 gtgaactcaa ataccgacag agatgcatct aacctatagt ctgtttcacc atggcttttg
    11881 atccgactgc cctctacaat gccaggtgca aaggacttaa actcggcttg tagtgttcta
    11941 aaagtgcttt ccactatgcc tttagcatcg ccacgtctag gtggagcact ttccactcgc
    12001 acattaaaac tagaaactaa ggcttcgacc tgatgactca ttaattcgcc acggtccgct
    12061 agcaacacat ctggcaaacc tacacacggc cagtctgagc tactaatctc aatatcatgc
    12121 tgggcacaaa tggccgtttt gtcagagcaa gcatttacaa aagcctgcat cgccaccaca
    12181 taagacggat tttcaaagcc gatataaaag cccgtgatca tccgactaaa cacatcaatc
    12241 acaatgtaaa gcgttggtct tcctatgatt ttttggcgat catgatgatc cactaaataa
    12301 atatcagcaa tcgtggcatc aatctcataa cgactcccag ggcctaacgc ctgagaagtg
    12361 gctgtactac ttaagggtcg tacgtctttt ttatataccc ctgctttaac tctagacttt
    12421 aagcgctgag ctttagggta ttctcgatca taaaaataac gaaactgacg tagtgttggg
    12481 taatcctctt ggggaatgcg aggaaaatac tgagcaaaca agtccacaaa tcgtctatag
    12541 gcaacggtgg tctttgtacc tttttgattt aacaggtgct tttctatggt caacctaaaa
    12601 aggcgttcaa tctcgggcgt taccttggtt ccttcaccct taccatattc tctggctcgg
    12661 ccaatctttg ctgttcctgt cgctgaacgt ctttcccctg gtgcaccgct gtttttgtag
    12721 tcaggaatta atgcattagg cgtttgacca cgctgccagt aacggcgtaa caacttataa
    12781 actgtagcct tagtaacctt atgttcttgg accacatgct caacgagttc gcttctgact
    12841 ttagggtcga aacgatcctt actattaata ataggaagaa tttttcgata atcctcctcg
    12901 cgcttctgga agctgacaga atctacagac ggctcttcta aatcaagatg tacataaggg
    12961 tcatcagttc taactaagcg gccttcatct aaatactgca ttagcaacaa ctcagcccta
    13021 gcttgtggaa ctcctttatc agcgcttatt tgcatccaga caacttggcc atcctctata
    13081 gccaaaatgc gatacggatc attatcaaat agcacaacct cattaatttg ccacatagcg
    13141 caactcctcc atattcacta cttggctaat acagagatct gcacacttat ttgccctgaa
    13201 agacttataa atattgaact taataaaacc atttgcggtt aaggctcgta tctcactcaa
    13261 tgttttgcct aactccaaat cataagcaat atcaacctgc ttacagacat tgataatgtt
    13321 ttcatctcct ttttcttgca ggatatgggc caatggggat agttgtgcta aaagctccgc
    13381 agaaacttct tctgttttca ctgaataaag ccattcaata ttttctttta ctacgggatt
    13441 tatttcttta tcagtaaaaa tgaaccaagg aatttgcttt tgctgccaat agcgacgctc
    13501 tagttctagt ttttctaagg tacgctcgtc ttgtaaggct gctgcaggtt tgacttgaat
    13561 agcaaactgc tcaaaaggac catctttgca gtccactaaa aaatcagtag acataacctg
    13621 atctacacca cgaataacag gatgcttaat accactatct attgcaatct gcctggtatc
    13681 actaggtaat aaggggaact gctcgcgtat atctagcacg ctgctctccc actcaagact
    13741 gagaaaaaca gcaagctcta agtcagatag caaatgatgg actcgtcccg tcttatgaga
    13801 ataaatacgg tgggaacgac ctgaagaagg aacttcttgt actgttagcc atggaatata
    13861 gtctttacca tgcccttggc cacgcccctc tttaatacga cgggcaattt gcacttcaga
    13921 aaatgaagag tttgctttag ccataacaaa agtccagtat gctttttcac agcataactg
    13981 gactgatttc agtttacaac tattctgtct agtttaagac tttattgtca tagtttagat
    14041 ctattttgtt cagtttaaga ctttattgtc cgcccacagc ccttttttat ctatgatagt
    14101 gattactcaa ccgtaaccga ttttgccagg ttacgcggct ggtcaacgtc ggtgcctttg
    14161 atcagcgcga catggtaagc cagcagctgc agcggaacgg tgtagaagat cggtgcaatc
    14221 acctcttcca catgcggcat ctcgatgatg tgcatgttat cgctacttac aaaacccgca
    14281 tcctgatcgg cgaagacata caattggccg ccacgcgcgc gaacttcttc aatgttggat
    14341 ttcagttttt ccagcaattc gttgttcggt gcaacgacga taaccggcat atcggcatca
    14401 ataagcgcca gcggaccgtg tttcagttca ccagcagcgt aggcttcagc gtgaatgtag
    14461 gagatctctt tcagcttcaa tgcgccttcc agtgcgattg ggtactgatc gccacggccc
    14521 aggaacagtg cgtgatgttt gtcagagaaa tcttctgcca gagcttcaat gcgtttgtcc
    14581 tgagatagca tctgctcaat acggctcggc agcgcctgca gaccatgcac aatgtcatgt
    14641 tcaatggagg catccagacc tttcaggcga gacagcttcg ccaccagcat caacagcaca
    14701 gttaactgag tggtgaatgc tttagtggac gccacgccga tttccgtacc cgcgttggtc
    14761 atcagcgcca gatcggattc gcgcaccaga gaagaacccg gaacgttaca gattgccagc
    14821 gaaccaaggt aacccagctc tttcgacaga cgcaggcccg ccagggtatc cgcggtttcg
    14881 ccagactgtg acaaggtgat catcaggctg ttacgacgca cggcagattt acgatagcgg
    14941 aattcagagg cgatttcgac gtcgcacgga atacctgcca gcgattcaaa ccagtagcgg
    15001 gaaaccatac cggagttata agaagtacca caggcgagga tctgaatatg ctcaaccttc
    15061 gacagcagtt cgtcggcgtt cggtcccagc tcgcttaaat caacctgacc gtggctgatg
    15121 cgcccggtaa gggtgttttt tatcgcgttc ggctgttcgt agatctcttt ctgcatgtag
    15181 tgacggtaaa tacctttatc gcccgcgtca tattgcagat tggattcgat atcctgacgt
    15241 tttacttccg cgccagtttt atcgaagatg tttaccgaac ggcgagtgat ttccgcaata
    15301 tcgccctctt caaggaagat aaagcgacgg gtcaccggca acagcgccag ctggtcagaa
    15361 gcgataaagt tttcgcccat acccaggcca atcaccagcg gactaccaga acgtgccgcc
    15421 agcagggtat ccgggtgacg ggagtccatg atcactgtac cgtacgcacc gcgcagctgc
    15481 gggatagcac gcagaacagc ctcacgcaga gtcccgcctt gtttcagctc ccagttcacc
    15541 agatgggcaa tcacttcggt gtcggtttca gaaacgaagg tatagccacg cgcttttaac
    15601 tcttcacgca gcggttcatg gttttcgatg atgccgttat gcaccaccac aatgtgttca
    15661 gaaacatgcg gatgcgcgtt agcttctgaa ggttcaccgt gggtcgccca gcgagtgtga
    15721 gcaataccgg tgccgccatc cagaggatgt tcttccgctg cctgagccag catctggact
    15781 ttaccgaggc gacgcaggcg ggtcatatga ccttccgcat caacaacggc cagaccggca
    15841 gagtcatatc cgcggtattc cagacgacgt aaaccttcaa gaaggatttc tgctacatca
    15901 cgttgcgcga tcgcgccaac aattccacac atagtttttg attccgattt atatcgttgt
    15961 cggtcaacct gtatgcccgt tttttcgggc gccccgagcc ttgtagagag tggggttatt
    16021 tttatagtta ctgcttgtgg gagggagatt atgttatctc ctcatcccag atgaccggat
    16081 tacccggcca gaattacttt ttctttaccg gacgacgcca gccttctttc tgagtctgcg
    16141 gcacacggct gatagctaat gcattttcgc cgacattacg cgtcacagtt gtacccgcag
    16201 caatggtcgc gcctttgcct actgttaccg gggccaccag ctgagtgtcg gaaccgacaa
    16261 acacatcgtc gccgataatg gtcttaaatt tattcgcacc atcgtagttg caggtaatgg
    16321 ttcccgcgcc gatgttaacg ttatcgccaa tttccgcatc gcccaggtaa gtcagatgcc
    16381 cgactttcga gcctttaccc agacgcgctt ttttcatctc aacgaagtta ccaacgtgtg
    16441 caccttccag caactcagca ccaggacgca aacgagcaaa cgggccaata gtacaggccg
    16501 ctgccagatt cgcgtcttcc acaacggtat acggactgat ttcgcaatca tcgccaatca
    16561 cgctgttttt aatcacgcaa ccggtgccga ttttcacgcg atgaccgaga gtcacgttgc
    16621 cctcgatgat aacgttagta tcaatttcaa catcgcgccc gtgagtaagc gtaccgcgca
    16681 gatcaaaacg cgccggatcg cgcagcataa cgcctgctaa cagcagtttt tcagcctgtt
    16741 cggactggta aacgcgctcc agacgggaga gttgcaggcg gttattcaca ccttctactt
    16801 cgcttaaacg ttgcggatga acggcgacga tttcacgccc ttcctgatac gccagcgcaa
    16861 taatgtcggt gatgtagtat tcgccctgag cattattgtt ggtcagcttc gccagccagc
    16921 gtttcatatc tgcgccgtta gcgatcagaa tgccggtgtt gatctcctga atctgacgct
    16981 gctcgtcggt ggcatctttg tgctcaacaa tgccggtaac tttgccattt tcacgggtga
    17041 tacgtccata accggtcgga tcatccagtt tcaccgtcag cagaccaatg ccaccctgcg
    17101 gtttagcatc acgcaggcgc tggagtgttt cgacagagat cagcggcacg tcgccgtaga
    17161 gcattaaaat gtcttcatca tcggcaaaga aaggtgcggc ctgctgcatt gcatgacccg
    17221 tacccagctg ctctgcctga agcacccagt taaggttgtc gtctttcagc gcctgtttaa
    17281 gcagatcgcc gccgtgaccg tacaccaagt gaacgtgcgc tgcgcctaat tcattcgcag
    17341 catcaatgac atgctgaacc atcgctttcc cggcaagggt atgcagcact ttcggaagat
    17401 cggaatacat gcgcgtgcct ttgcctgcgg caaggatcac tacgctcata gcattattca
    17461 acatacgcgt cctgactgta atttgagaac gaatttaaac cgcttcacct tgaaaaaact
    17521 acattttttt catcgtgaaa tggacagagg ataaattgtt caataacgga ttatccccgc
    17581 tgacgaaagt gccattttcg accatcgtca cgccttttgt ctgcagaaaa ttaaggcagt
    17641 accttcattt aagcgtcagg tggatgtttt tgctcttatt tcgatcaatg aataaacggg
    17701 aaaacagggg tgttttatat cagcaggatc tatgtgaacg ctcttcagga cggatcacgc
    17761 gtaaaaaaaa gccagcctgt ttccagactg gcttttgtgc ttttcaagcc ggtgttacat
    17821 cgcttttttg gtcaactcga taacgcgcag ctgcgcgatc gctttggcca gttccgcaga
    17881 cgcctgagcg taatctacgt cgccgtgaga gctgctaatg tgctcttcag ccttacgttt
    17941 cgcttccatg gctcgcgctt cgtcgagatc ctggccgcga attgcggtgt cggccagaac
    18001 ggtcacgttg ccaggctgca cttcaagaat gccgccagac agatagataa actcttcgtg
    18061 accgtgctgt ttcacgatgc gaatcatacc aggcttaatg gcggtgagca gcggtgcgtg
    18121 gccagggtag atccccagtt caccttcgct acccgttacc tggattttct cgaccagacc
    18181 agagaacatt tgttgctctg cgctgacgac gtccaggtgg taagtcattg ccatatcacc
    18241 ctccgattaa ggcgttaaag ttttttggct ttttccacag cttcttcgat ggaaccgacc
    18301 atgtagaacg cctgctccgg caggtgatcg tattcgcctt ccatgatgcc tttaaagcca
    18361 cggatggtgt ctttcaggga gacgtattta cccggagaac cggtgaatac ttctgccacg
    18421 aagaacggct gggacaggaa gcgctggatc ttacgagcac gcgctaccac cagtttgtct
    18481 tcttcagaca gttcatccat acccaagatg gcgatgatgt ctttcagttc ctgataacgt
    18541 tgcaggatgg actgaacgcc acgcgcggtg tcgtagtgtt cctgaccaac caccagcggg
    18601 tccagctgac ggctggtgga gtccagcggg tcaacggccg ggtagatacc cagagacgcg
    18661 atctgacggc tcagtaccac ggttgcgtca aggtgcgcaa aggtggttgc cggagacggg
    18721 tcagtcaagt catccgcagg tacgtatacc gcctgtacgg aggtgataga accagttttg
    18781 gtggaggtga tacgttcctg cagaacgccc atctcttccg ccagggtcgg ctgataacct
    18841 accgctgaag gcatacggcc cagcagtgcg gatacttccg taccggccag ggtgtaacga
    18901 tagatgttgt caacgaacag cagaacgtca cgaccttcgt cacggaattt ctcagccatg
    18961 gtcagaccgg tcagagcaac gcgcagacgg tttcccggcg gctcgttcat ctggccatac
    19021 accagggata ctttgtcgat aacgttggag tcggtcattt cgtggtagaa gtcgttaccc
    19081 tcacgagtac gttcacctac gcccgcaaac acagagtaac cggagtgctc gatcgcgatg
    19141 ttacgaatga gctccatcat gtttacggtt ttacctacac ccgcaccacc gaacagacca
    19201 actttaccgc ccttagcgaa cggacacatc aggtcgataa ctttgatacc ggtttccagc
    19261 agttcctgag agtttgacag ctcttcgtag gaaggtgctg cgcggtgaat cgcccaacgc
    19321 tcttcttcac cgatctcgcc tttcatgtcg accggttcac ccagtacgtt catgatacgg
    19381 cccagagtcg ctttacctac cgggacttcg atcgggtgtt cgaggtcttt tacatccaga
    19441 ccgcgacgca gaccgtcgga ggaacccatt gcgatggtac gcacgatacc gccgccgagc
    19501 tgctgctgaa cttccagcac cagacgctca ttaccatttt gcacctcaag agcatcgtac
    19561 acgcgcggta cggcatcctg agggaattcg acgtcaacta cggcgccgat tacctggaca
    19621 atctttccag tagccatctt aaatcctcta cgaaataacc tgtttaaacc gcggcggccc
    19681 ccgagacgat ctcggtgagt tcctgagtaa tgctggcctg acgagctttg ttgtatacca
    19741 actgcagctc tttaatcagg ctgccgccat tgtcggtcgc ggctttcatc gccaccatac
    19801 gggcggcctg ctcgctggcc aggttttcaa ccacgccctg ataaacctga gattcgacat
    19861 aacgacgcag cagggtatcc agcaacgcct tcggatcggg ttcgtacagg taatcccagg
    19921 atttatgttt cagatcatca tcatctgatg ccggtaacgg cagcagctgg ctgatggtcg
    19981 gaacctgaga catggtgtta ataaatttgt tgctgacaat gtaaagcttg tccagacggc
    20041 cttcgtcgta agcctgcaac atcactttta ccggaccgat cagttcggac agggaagggt
    20101 tatcccccat gccggtgacc tgggcaacaa cattgccgcc cacggagttg aagaacgaca
    20161 cgcctttcga gccgatcatt gcgaggtcgc attgaacgct tttgtcggtc caggtcttca
    20221 tttccgccag tagttttttg aacaggttaa tgttcaaacc accgcataaa ccacggtcgg
    20281 tcgacaccac caggtagccc acgcgtttaa cgtcgcggtc ttccaggtaa gggtgcttat
    20341 attccagatt accgtgtgca aggtgaccaa tcactttgcg catggtttct gcataaggac
    20401 ggctggccgc catgcgatcc tgcgatttac gcattttgga agcggcgacc atctccatcg
    20461 ctttagtgat cttttgcgtg ttctggacgc ttgcgatctt actacgtatc tcttttgcgc
    20521 cggccatgag cttctcctca atgccttgcg gcctgcccta aggcaagccg ccagacgtta
    20581 ccaggattgg gttgctttga aggaatcgag gatgcctttc agcttgcctt cgatttcgtc
    20641 gttgtagcca ccggtctggt tgatctcttg catcaacgga gcgtgatcac ggtcgacgta
    20701 agccagcaga gcggcttcga agctgccaat tttcgacagt tcaacatccg ccaggtaacc
    20761 acgttctgct gcgaacagaa ccagagactg ctgcgcaacg gacatcggcg catactgttt
    20821 ctgtttcagc agttcggtca ctttctgacc gtggtcaagc tgcttacgtg ttgcatcgtc
    20881 aaggtcggat gcaaactgag agaacgctgc cagttcacga tactgtgcca gagcggtacg
    20941 gataccaccg gacagttttt tcatgatctt ggtctgtgct gcaccaccaa cacgggatac
    21001 ggaaataccc gggttaaccg caggacgaat accggcgttg aacaggttgg tttccaggaa
    21061 gatctgacca tcggtaatgg agattacgtt ggtcggaacg aacgcagaaa cgtcacccgc
    21121 ctgagtttcg ataatcggca gtgcggtcag agaaccggtt ttccctttca cttcaccttt
    21181 ggtgaaggct tcaacgtatt cggcgttaac acgtgcagca cgctccagca gacgagagtg
    21241 gaggtagaaa acgtcgcccg ggaatgcttc acgtcctggc ggacgacgga gcagcaggga
    21301 gatctgacgg taagcaacag cctgtttaga caggtcatcg taaatgatca gcgcatcttc
    21361 accgcggtca cggaagtatt cgcccattgc gcaaccggca tacggtgcca ggtattgcag
    21421 tgcagcggat tcagacgcgg ttgctaccac aacgatggtg ttagccagtg cgccgtgctc
    21481 ttccagttta cgtaccacgt tagaaatggt ggacgctttc tggccgatag cgacatagat
    21541 acatttgata ccggaatcgc gctggttgat gatggcatcg atagccagtg cggttttacc
    21601 tgtctgacgg tcaccgatga tcaattcacg ctgaccacga ccgattggga tcatggagtc
    21661 aacggcttta taaccggtct gtaccggctg atctacggac tgacgttcga taacgcccgg
    21721 agcgattgct tctacagcag agaagccgtc gtgatccagc ggacctttac cgtcgattgg
    21781 tgcacccaga gtgttaacca cacggcccag caggccacgg ccaaccggaa cttccaggat
    21841 acggccagta cacttaactt tcatgccttc ggcaaggtca gcgtacggac ccataacaac
    21901 cgcacctaca gagtcgcgct caaggttcag tgcgatagcg taacggttac ccggcaggga
    21961 gatcatttca ccctgcatac aatcggccag gccgtgaatg cggataacac cgtcacttac
    22021 agaaacaata gtaccttcgt tgtgagcttc actcacaaca ttgaactgag caatgcgctg
    22081 cttgatcagt tcgctgattt cggtggaatt cagttgcatg ctccagtccc cttaagactg
    22141 caagacgtct gcaaggcgct caagacgacc gcgtacgctg ccatcaatga ccatatcacc
    22201 cgctcggatg ataacgcctg ccattacaga cttatcgatt ttgcaattca gcttaacttt
    22261 gcgtgacaga cgtttttcca tcgcagcaga aattttcgcg agctgttgtt cactcagtgc
    22321 ggcagcggaa atgacgtcta cctcagcggt agcctcactc acggcacgaa ggtgaataaa
    22381 ctgctccaga acatccggga gcgcgttaag acgaccattt tcagccatta cccgaatcag
    22441 gttctgaccg ttttcgtcca gttgctcacc acaaactgcg ataaacgact cggcgagcgt
    22501 ttctggcgca agcgcgccag agagaagctc tgccatttgt tcgtttttgg ttacctcggc
    22561 ggcaaacgcc agcatgtcct gccagcgttc tacactttgg tgttcgacgg caaagtcaaa
    22621 agctgctttg gcgtaggggc gagctaccgt aataaattca gacatcagcc cctccctcct
    22681 tacagttcag cgacaagttt atccacgatg tcgctgttag cagcttcatc cacggaacgt
    22741 tcgatgatct tctcggcgcc agcaacagcc aggatagcaa cttgcttacg cagctcttca
    22801 cgggcacgtt tacgctcggc ttcaatttct gcctgcgcct gggccacgat tttagtacgt
    22861 tcctgttctg cctcagcttt cgcttcgtcc agaatctgcg agcggcgttt gttcgcctgc
    22921 tcgatgatta cctgggcttc cgctttcgct tttttcagct ggtcggtcgc gctggccttt
    22981 gcaaggtcaa ggtccttatg tgctcgttct gcggaagcaa ggccgtcagc aatttctttt
    23041 tgacgttttt cgatggctgc cattaatggc ggccatacgt acttcatgca gaacagaacg
    23101 aacaggacaa acgcgatggc ctggccgagg attgttgcgt taagattcac agcacaatgc
    23161 ctctatttag ttaacgttct gatattgctc tttaaataaa agcaacgctt actacgcgac
    23221 agcgaacatc acgtacagac ccagacctac agcgatcatc gggatagcat ccaccagacc
    23281 cataacgata aagaactgag tacgcagcag aggaatcaga tcaggttgac gcgctgcgcc
    23341 ttccaggaat ttacccccga ggatgccgat accgatcgca gcaccgattg ccgccagacc
    23401 catcatcaca gcggcagcca tgtacagcag atccatattc aggttttcca tgacagtctc
    23461 cagtttgttt cagttaaaac gtagtagtgt tggtaaatta atgttcttca gacgccatcg
    23521 acagatagac gatcgtcaga accatgaaga tgaaggcttg cagcgtaatg atcaggatgt
    23581 ggaaaatggc ccacggcaca ttcaggatcc actgtgacca ccacggcaac agaccagcaa
    23641 tcagaatgaa aatcagctca ccggcataca tgttaccgaa cagtcgcaaa ccgagtgaaa
    23701 ctggtttgga cagcaggctt accccttcaa ggattaagtt gacaggaatg aacgcccagt
    23761 gattgaacgg ctgcagcgtc aactctttcg tgaagccgcc gatgcctttc attttgatgc
    23821 tgtagaacag aatcaggata aatacgccca gtgccataga cagcgttacg ttcacgtccg
    23881 cagacggaac cacacgcagt gcaggcagac ccagtacatg ttcagcaatg tacggcagca
    23941 ggtcgatagg cagtaaatcc atcaggttca tcaggaatac ccagacgaag atcgtcaggg
    24001 ccagcggagc aatcagcttg cttttgccat ggtacatgtc tttcacgcta ccattaacaa
    24061 agccgatcac cagctcaatc gcggtctgaa acttacctgg cacaccgctg gtcgcctttt
    24121 tggctacgct acggaataaa accaggaaca acagacccag caccaccgag aagaacatgg
    24181 agtcaatatt gattgtccag aaggtggctg gggggttttg tggatccacc agcgagaatg
    24241 tacgcaggtc cagctgaagg ttattcaggt ggtgtcctat gtaatcctgc ggcgtcatat
    24301 tttctgaagc catgatgcct tttacccttt gttgttaatt acagccggtg ccagtatctg
    24361 aaccaccagc accaaaaccc acgtaacgat cagcggcaag aataccgcct ttaaaaccgc
    24421 caacgccacc accagtaaca ccaacatcgc cagaactttg aaagcttcgc caaatgcgaa
    24481 tgtccaggcc acccggcctt tcgctggtgt atacgcctgg tgacgccagg caaatatcat
    24541 aaacaaaacg ttaggcagaa agactgccag gcccccgctt attgcagaga cgccccagaa
    24601 ggggtctttg aggctgaaca gcaatccact tgctatcacc accagtaact gaacgagcag
    24661 aagcttccga gcaacgtttc gactcacgag cgacacagac atcacgtttt tcactcctgc
    24721 tcccttcgag gtatgccgcg tgtcgtataa aactttcttt aaggcttaga gtcaagcatc
    24781 aaaaagcggt caaattatac ggtgcgcccc cgtgatttca aacaataagt agccaaaagg
    24841 tgaataaatg tttaaatatt tttccagtgc atacaattgc gacttttccg ctaaccctgt
    24901 tcgatcatga aaaactgtaa ataacgcgta aacactagtg ataaagcgtg cttcagatca
    24961 catattgcgc atgttcgcgc acagtatatt tatttacttg gcaaatgatg cctttgcaag
    25021 tttatgatat ttcagtctaa aaacagatac tgttttaata aatgacattt acacaacaaa
    25081 aaccacccat tgacattttt aataatgttt taacagccaa tgatggttct tagcgccgat
    25141 ttttagcaga ctgatatttt cactaatgac ttattttctg cttaccaaaa aaagccacgt
    25201 tatcttgttg atacaaaaga gtgaacgtgg cgttaaatgt aaccagttat atcagtagaa
    25261 aacttggttg ttgttaacag tctaaccggt caatttttta tgattttttt gataaaaatt
    25321 aaattttatt tgctttaatc accaccagat gacgttcgcc atccagagct ggaacctgaa
    25381 gtttaaccac tgattcgacc tgatattctt cgggcaacaa agcgatttca tcttccggca
    25441 tttgcccttt cagcgcgtag aaacggcctt gctcaccagg aagatggtgg caccagctca
    25501 ccatatcgtt cagagaggca aaagcgcggc taattacgcc atcaaatggc ggctctgaag
    25561 gaaactcttc taccctgctc tgtactggct caatattctc cagtttaagc tcatgttgca
    25621 cctgacgaag gaaacgcacg cgtttaccaa ggctatccaa cagagtgaaa tgggcttcag
    25681 gacgcacgat agagagtgga atgcctggca gtcctggtcc ggtgccgaca tcgataaacc
    25741 gttcaccttg cagatacggt gccaccacaa tgctatcgag aatatggcgt accagcatct
    25801 cattaggatc gcggaccgaa gtcaggttgt acgctttgtt ccatttatgc agcatattca
    25861 cgtaggcaat aagctggttt ttctggtgat cggtaagcga aatacctgcg tctttcagca
    25921 gtaaggagag tttgttgagc acggtgatta cctgttcttg atgcgttgcc tggtaagcgg
    25981 gtgcttacca ggcattttta atgcgttatg cgctacgacg cagcataccc tgttttttca
    26041 gccacaccag cagaatggag atagccgcag gcgtgacgcc agaaatacgc gaagcctggc
    26101 cgatagaggc cggtttgtga tcgttaagtt tggcgatcac ttcgttagaa agaccggata
    26161 cctggcggta atccagtgtc gcgggtagca gggtgttctc gttacgcagc tgcttttcga
    26221 tctcatcttg ctggcgcgcg atataacctt cgtatttaac ctgaatctca acctgttccg
    26281 ccgcctgttc gtctgtcaac gcaggggcaa acggcgtcag cgtggttaat ttttcataag
    26341 tcatttccgg acgacgcagc agatcttcac cactggcttc acgggaaagc ggcgcagtca
    26401 ggtgagcatt cacttcggct gcagcttccg ccgacggggt tacccaggtc gatttcagac
    26461 gctgacgctc acgctcgata ttctcaagtt tctcgttaaa gcgcgcccaa cgttcgtcat
    26521 ccaccaggcc cagttcacga ccgatttcag tcaaacgcag atccgcatta tcttcgcgca
    26581 gcatcagacg atattctgcg cgcgaagtaa acatacgata cggttctttg gttcctaaag
    26641 tgcacaggtc atcaaccagt acgccgagat acgcctgaga acgcgccgga gcccaacctt
    26701 ctttgtcagc agacagacgg gcagcgttaa gaccgaccag caaaccttgc gcagcggctt
    26761 cttcgtaacc ggtagtgccg ttaatctgac cagcaaagaa cagcccctgg ataaacttgc
    26821 tctccagcgt cggtttcagg tcgcgtggat cgaagaagtc atactcaatg gcataacccg
    26881 gacgcacgat cttcgcgttt tccatcccct gcatggagcg gacgatttgc atctgcacat
    26941 cgaacggcag gctggtggag ataccgttcg gataaatttc gttagaggtc agcccttccg
    27001 gttcaaggaa gatctgatgc tgatttctgt cggcgaagcg catgactttg tcttcgatcg
    27061 atgggcagta gcgtgggccg acaccttcga tcacccctgc gtacattggg ctacgatcga
    27121 ggttactgcg gatcacatca tgggttttct cgttggtatg agtgatataa cacggcacct
    27181 gctggggatg ctgggacgca ttgcccataa acgagaatac cggcattggg ttatcgccat
    27241 gctgttgcgc cagcacacta aagtcgatag ttcgagcatc aatacgcggt ggtgtcccgg
    27301 ttttcagacg accaacgcgc agcggcagtt cacgcaaacg gcgagaaagc ggaatggacg
    27361 gcggatcacc agcacgacca ccgctgtaat tatccagacc gatatgaatt ttaccgtcga
    27421 ggaacgtccc aacggtgagc acgacggctt tggcacggaa cttcagtccc atttgggtaa
    27481 cagcaccgac cacgcgatcg ttttcgacaa taagatcttc aaccgcctgc tggaagatca
    27541 tcaggttcgg ttggttctcc agcgccgtac gtaccgcctg acggtagagc acacgatccg
    27601 cctgagctcg ggtagcgcga accgccggtc ctttgcttgc gtttagtatc ctaaactgga
    27661 tacccgcttg atcgatcgct ttcgccatca gaccgccgag tgcatccact tcttttacca
    27721 gatgtccctt cccaataccg ccgatcgccg ggttgcagct catctgcccc agagtgtcga
    27781 tattgtgtgt caaaagcaga gtctgttgac ccatacgcgc cgcggccatc gcggcctcgg
    27841 tgcctgcatg acccccgcca atgatgatga cgtcaaaagg atccggataa aacatggtga
    27901 ttgcctcgca taacgcggta tgaaaatgga ttgaagcccg ggccgtggat tctactcaac
    27961 tttgtcggct tgagaaagac ctgggatcct gggtattaaa aagaagatct atttatttag
    28021 agatctgttc tattgtgatc tcttattagg atcgcactac cctgtggata acaaggatcc
    28081 ggcttttaag atcaacaacc tggaaaggat cattaactgt gaatgatcgg tgatcctgga
    28141 ccgtataagc tgggatcaga atgaggggtt atacacaact caaaaactga acaaccgttg
    28201 ttccttggat aactaccggt tgatccaagc ttcctgacag agttatccac agtagatcgc
    28261 acgatctgta tacttatttg agtaaattaa tccacgatcc cagccattct tctgccggat
    28321 cttccggaat gtcgtgatca agaatgttga tcttcagtgt ttcgcctgtc tgttttgcac
    28381 cggaattttt gagttctgcc tcgagtttat cgatagcccc acaaaaggtg tcatattcac
    28441 gactaccaat accgattgcg ccaaagcgga ccgcagaaag atcgggcttc tgttcctgca
    28501 atgcttcata gaaaggagaa aggttgtccg gaatatctcc ggcaccgtgg gtggagctga
    28561 taatcagcca gatccccgag gcagataaat cttccaacag cggaccgtgc agcgtttcgg
    28621 tggtaaaacc cgcctcttcc agcttttcag ccaggtgttc tgctacatat tcggcaccgc
    28681 cgagggtgct gccgctgata agagtgatat ctgccataaa ccgccacctt tattaagagt
    28741 ggcgtattgt acgctgtgaa cgcgttggga tctacctgtg gaaaagtatg ggattaaaaa
    28801 agccgattat ggcttgatgg tacgcatgat cgggttttgc aggacgatca atgtctcggt
    28861 ggactgaatt tcatcaattg tttggatctt gttgataagt acatgctgga gagcgtcgat
    28921 cgaacggcac attactttta taaagatgct gtagtggcct gttgtgtaat aggcttcagt
    28981 gacttcagca aggctttcca gctttgccag cgcggaaggg tagtctttgg cgctctttaa
    29041 tataatgccg ataaagcagc ctacgtcata accgagctgc ttcgggctga catcaatacg
    29101 cgccccggta atgatccccg cctgcttcat tttctctact cgaacgtgaa tcgtccccgg
    29161 actgacgcca aattgtttcg ccagttcggc gtaagcggtg cgcgcattgc ccattaatgc
    29221 ttccaggatg ccacggtcca gattgtcgat cagataattt tccataggat tttcttatgc
    29281 agattgatga ttcattctat tttagccttc ttttttaatg aatcaaaagt gtgttaggct
    29341 ttttattgaa tgattattgc atgtttgtcg gtttttgttg cttaatcata agcaacagga
    29401 cgcaggagta taaaaaatga aaaccgctta cattgccaaa caacgccaaa ttagcttcgt
    29461 gaaatctcac ttttctcgcc aactggaaga acgtctgggg ctgatcgaag tccaggcgcc
    29521 gattcttagc cgtgtggggg atggcacgca ggataacttg tcgggctgtg aaaaagcggt
    29581 gcaggtaaaa gtgaaagctc tgcctgatgc ccagttcgaa gtggttcatt cactggcgaa
    29641 gtggaaacgt cagaccttag ggcaacacga cttcagcgcg ggcgaagggc tgtacacgca
    29701 catgaaagcc cttcgccccg atgaagaccg tctttctccg ttgcactcgg tctatgttga
    29761 ccagtgggac tgggaacgcg taatgggcga cggtgagcgt caattctcga ctctgaaaag
    29821 cacggtagag gcgatctggg cgggaattaa agcaaccgaa gctgcggtta gcgaagagtt
    29881 tggcctggca ccgttcctgc cggatcagat ccacttcgta cacagccagg agttactgtc
    29941 tcgttatccg gatctcgatg ccaaagggcg tgagcgggcg atagcgaaag atcttggcgc
    30001 ggtattcctt gtcgggattg gcggcaagct gagcgatggt catcgccacg acgtgcgcgc
    30061 accggattat gatgactgga gcaccccgtc agagctgggt catgcgggtc tgaacggcga
    30121 tattctggtg tggaaccctg tactggaaga tgcgtttgag ctttcttcca tggggatccg
    30181 cgtggatgcc gacacgttga agcatcagtt ggcgctgacc ggtgatgaag atcgtctgca
    30241 gctggaatgg catcaggcgc tgctgcgcgg tgaaatgccg cagaccatcg gcggcggtat
    30301 cggccagtct cgtttgacca tgctgctgct gcaactgccg catatcggcc aggttcagtg
    30361 tggtgtatgg ccagctgctg ttcgcgagaa cgtcccttct ctgctgtaat aatttatcgc
    30421 cgccagcgtc tgagcaggcg gcttcgcatc ccggtatcaa agcgccagat atgatcgaaa
    30481 atgcgcatga tgccgggttt gccgtgtgcc gacatcgcca cggcatgaaa gcgatgctga
    30541 tgtacccgct gcagctcttt cactttactc gtcacgtcgt caggcaaccg ctgagcgata
    30601 aaatcagaaa tcaccaccgc atcggcatca aaccattccc tgctttgcaa gcgttccata
    30661 atggcgcgaa aacaactggc aagatcggtg ccgccacgaa actgctggct taaaaaacgg
    30721 attgcttgtt cgatgccttg ggggcctgaa agctcataac ggacgatctc ggtggaaaat
    30781 agcataatat agcagcgccg gttttcagcg agagcaatgc gcatcaaggc caggcagaac
    30841 gctttcgcac actgttcatt aaagccgccc attgagccgg aagtatccac acagacaata
    30901 aacggcccgc gcggctgttc atcgtaatct ttatgtacca ccggacgttc gatcactttt
    30961 tcacgccacg actcaccgtg caggcgatag gtgagcaact gtttttccac cagccgacgg
    31021 taaaactcat actccagttc cgttatccct agtgtcgcca gttctggcgg cagaagacgt
    31081 aaaatatcat cgctttgttg cagaccatca acctgctcag gaaccgtcgc cggttcgcgc
    31141 accatggtgc ggaaggtttc catctgcgca tcgttgcgcg gtattgattt ggcttcccga
    31201 gaacgcccca gctgctctgc caggcgtttc agttccggct gttcgttaag aaattcaccg
    31261 tatttcacaa tcaactgata gtcgccacgt ttaagctgac cggcgctcat atcccacaga
    31321 cgaccagctg cagtattgtt atctgcgaga atcggttcaa gttgtccgct cagcgtcatg
    31381 cgttcttgaa cttcactcaa cagttgttcg cgttcttctt ctaatagctg ttgatttaac
    31441 gtcgttgctt gcacgatcag acttaaacgc caacgctgga gaaaaagcgt gtgtaacgct
    31501 gaagttattg tgctgttagc atcaaccaac tgccgggctt gttctgccca tggggaattc
    31561 agacgatgca gtaagtccag gatctgtggt agctgcacaa taaactgcgg cgtggagagg
    31621 agctggcttt gctgatagca catcacctct tcggtgagtt ccggcgggac tcgggcatct
    31681 ttcagccgac tgcgcagcgc ctcacgccag cggggaacat catcagtgat tgccgccttc
    31741 aggcgtggga atttttcaaa gaagaccgcc agctgcggtg aggccagcag tgcgatgatc
    31801 atctcttcga tcaatccctc ttcgctgacg gccagcatca cattaagcgt atccagcgtt
    31861 agcattgttg tgcctggtga atctgtgcac cgacatcctg taggctggct tcaatgcgac
    31921 ctaaccagtc gccagggata aacagacatt tttgctgttc gctgaataaa gcgtgttgct
    31981 tgcgccagtc gctttcaagc tcttccagtt gttgttttat ttcaccaggc agaccttcag
    32041 ccgatgaacc gggaagtgcc agcgtactgc cttgtaaact tacatcgcga acgacaagat
    32101 gttgggcgct atcaacttcc agattcagtt tctgggcaaa gccgataccg ttcagtttac
    32161 cgcgaatttc accacctttg ctcagccact gttccagcgc gctacgctca aaggagatat
    32221 gaaccacttc catatcatgc agttttaacg gtttttgcag cagtagagtc agagtggaag
    32281 cagtaacgtt aacaggaagt tgatactgct gacgacggct gaaaatgccg cccagacgaa
    32341 ttaccgttaa ggctgtttta tcgctttgtt gctgctggag ttgcaggtga cgttgcacaa
    32401 tcgcgccaag gcgagtcaac atcccttgct gttgccaggc atgaccggtc atcaatacat
    32461 caatttgttg ttgtatcaaa ttcaggcttt gcgcgtcata ccacaggcaa tctttcagca
    32521 aaatgagatc taccggggca acagcactgc gaccgctaaa aaaggcgctg gcctgcaata
    32581 atcggatcgc ttttttccag cgacgatccg agacataagg cgcatccggt aatttatcca
    32641 gttgcaggcg cagcataaaa atcagctcaa atacatgatc gggcagcgta atttcaccaa
    32701 tctctttctg ccagcgttca tattcttcat ctgtgacctg caaggcatca ggaaccggat
    32761 tgtcgttttc atcctgttga ctggtcagca tggagcggaa attcgcttta tcctgcactt
    32821 tatctaacca cagacgaatc agcatgcggt catataacgc ttccagactg ctgtctgctt
    32881 ccggcagctc gttggaggcc gccaccagca gacgcatcgg gattttttct acgtgtgcac
    32941 cgttgcggaa ctggcgctcg ttaatggcgg tgagcaaggt attaagaatt gccgggcccg
    33001 ctttccagat ctcatccaga aagacaattt ctgcttccgg caggtaaccg ctggttaaac
    33061 gttcatagcg cccttcatct tttagcgcct gaatagaaag gggaccaaaa acttcttccg
    33121 gcgtggagaa gcgggtcatc agatattcaa acgcgcgggc attctgaaag gcgaatttta
    33181 agcgccgggc gatcaaactt ttggcaatac ctggcgggcc aaggaggaac acactttcac
    33241 cacttaatgc cgctaataaa cacaagcgga tggcgtggct acgttcataa agcccctttt
    33301 ccaacgaact gctcaggcgg gaaattcttt ccgctaataa atgagggtga gccataatga
    33361 agtggcgtcc tttcgtcaaa agttctgcgt aaattgcgag tatagacgtt tcttgctggt
    33421 ggctaaaata gtctcaaagg
//
LOCUS       DADMKF010000031        32459 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.032, whole genome shotgun sequence.
ACCESSION   DADMKF010000031 DADMKF010000000
VERSION     DADMKF010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32459
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.032"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            424..744
                     /gene="cyaY"
                     /locus_tag="JNC24_001332"
     CDS             424..744
                     /gene="cyaY"
                     /locus_tag="JNC24_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HAY9158127.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDWDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(784..3330)
                     /gene="cyaA"
                     /locus_tag="JNC24_001333"
     CDS             complement(784..3330)
                     /gene="cyaA"
                     /locus_tag="JNC24_001333"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HAY9158128.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNMPPANQDHDTPLLQQYFS"
     gene            3717..4658
                     /gene="hemC"
                     /locus_tag="JNC24_001334"
     CDS             3717..4658
                     /gene="hemC"
                     /locus_tag="JNC24_001334"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HAY9158129.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLRALVGAPDGSQIIRGER
                     RGAPQNAKQMGISLAEELLNNGAREILAEVYNGDAPA"
     gene            4655..5395
                     /gene="hemD"
                     /locus_tag="JNC24_001335"
     CDS             4655..5395
                     /gene="hemD"
                     /locus_tag="JNC24_001335"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HAY9158130.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGQQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPRLPNYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTARGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTTVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNAANDALLRALQ"
     gene            5417..6634
                     /gene="hemX"
                     /locus_tag="JNC24_001336"
     CDS             5417..6634
                     /gene="hemX"
                     /locus_tag="JNC24_001336"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HAY9158131.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAAAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQATQLE
                     QANRQQETLAKQLDEVQQKVATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTAAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPAPA
                     PQGDTPAAAPQGE"
     gene            6637..7833
                     /gene="hemY"
                     /locus_tag="JNC24_001337"
     CDS             6637..7833
                     /gene="hemY"
                     /locus_tag="JNC24_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HAY9158132.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            complement(8740..9975)
                     /locus_tag="JNC24_001338"
     CDS             complement(8740..9975)
                     /locus_tag="JNC24_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HAY9158133.1"
                     /translation="MLQQVPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYVRHYIAASEPQNEVAFTWQGGEPTLLGLDFYRRAVKLQAKYGAGRKISN
                     SFQTNGVLLDDKWCAFLAENHFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAARDGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLLVVNK"
     gene            complement(10122..10198)
                     /locus_tag="JNC24_001339"
     tRNA            complement(10122..10198)
                     /locus_tag="JNC24_001339"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10162..10164),aa:Pro,seq:tgg)
     gene            complement(10241..10327)
                     /locus_tag="JNC24_001340"
     tRNA            complement(10241..10327)
                     /locus_tag="JNC24_001340"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10291..10293),aa:Leu,seq:cag)
     gene            complement(10348..10423)
                     /locus_tag="JNC24_001341"
     tRNA            complement(10348..10423)
                     /locus_tag="JNC24_001341"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10388..10390),aa:His,seq:gtg)
     gene            complement(10482..10558)
                     /locus_tag="JNC24_001342"
     tRNA            complement(10482..10558)
                     /locus_tag="JNC24_001342"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10522..10524),aa:Arg,seq:ccg)
     gene            complement(10659..12044)
                     /locus_tag="JNC24_001343"
     CDS             complement(10659..12044)
                     /locus_tag="JNC24_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAY9158134.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWFPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(12235..12975)
                     /gene="rffM"
                     /locus_tag="JNC24_001344"
     CDS             complement(12235..12975)
                     /gene="rffM"
                     /locus_tag="JNC24_001344"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HAY9158135.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(12978..14330)
                     /gene="wzyE"
                     /locus_tag="JNC24_001345"
     CDS             complement(12978..14330)
                     /gene="wzyE"
                     /locus_tag="JNC24_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen assembly polymerase"
                     /protein_id="HAY9158136.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNREPNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(14327..15406)
                     /gene="rffT"
                     /locus_tag="JNC24_001346"
                     /gene_synonym="wecF"
     CDS             complement(14327..15406)
                     /gene="rffT"
                     /locus_tag="JNC24_001346"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HAY9158137.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDRSNDHIAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASVDKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(15403..16653)
                     /gene="wzxE"
                     /locus_tag="JNC24_001347"
     CDS             complement(15403..16653)
                     /gene="wzxE"
                     /locus_tag="JNC24_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HAY9158138.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(16655..17785)
                     /gene="rffA"
                     /locus_tag="JNC24_001348"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(16655..17785)
                     /gene="rffA"
                     /locus_tag="JNC24_001348"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HAY9158139.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDALAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGERFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(17790..18464)
                     /gene="rffC"
                     /locus_tag="JNC24_001349"
                     /gene_synonym="wecD"
     CDS             complement(17790..18464)
                     /gene="rffC"
                     /locus_tag="JNC24_001349"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HAY9158140.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAQLLTPDVLAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASGRFYAQWIENAVRGTFDHQCLILRAASGGIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(18442..19323)
                     /gene="rfbA"
                     /locus_tag="JNC24_001350"
     CDS             complement(18442..19323)
                     /gene="rfbA"
                     /locus_tag="JNC24_001350"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HAY9158141.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     DIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(19342..20409)
                     /gene="rffG"
                     /locus_tag="JNC24_001351"
     CDS             complement(19342..20409)
                     /gene="rffG"
                     /locus_tag="JNC24_001351"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HAY9158142.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELAPN
                     KPHGVVHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(20409..21671)
                     /gene="wecC"
                     /locus_tag="JNC24_001352"
     CDS             complement(20409..21671)
                     /gene="wecC"
                     /locus_tag="JNC24_001352"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HAY9158143.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSTRASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEVLATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(21668..22798)
                     /gene="wecB"
                     /locus_tag="JNC24_001353"
     CDS             complement(21668..22798)
                     /gene="wecB"
                     /locus_tag="JNC24_001353"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HAY9158144.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIDPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQTMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(22854..23900)
                     /gene="wzzE"
                     /locus_tag="JNC24_001354"
     CDS             complement(22854..23900)
                     /gene="wzzE"
                     /locus_tag="JNC24_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HAY9158145.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(23912..25015)
                     /gene="wecA"
                     /locus_tag="JNC24_001355"
     CDS             complement(23912..25015)
                     /gene="wecA"
                     /locus_tag="JNC24_001355"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HAY9158146.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(25255..26514)
                     /gene="rho"
                     /locus_tag="JNC24_001356"
     CDS             complement(25255..26514)
                     /gene="rho"
                     /locus_tag="JNC24_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HAY9158147.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(26599..26700)
                     /gene="rhoL"
                     /locus_tag="JNC24_001357"
     CDS             complement(26599..26700)
                     /gene="rhoL"
                     /locus_tag="JNC24_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide rhoL"
                     /protein_id="HAY9158148.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(26841..27170)
                     /gene="trxA"
                     /locus_tag="JNC24_001358"
     CDS             complement(26841..27170)
                     /gene="trxA"
                     /locus_tag="JNC24_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HAY9158149.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            27301..28566
                     /gene="rhlB"
                     /locus_tag="JNC24_001359"
     CDS             27301..28566
                     /gene="rhlB"
                     /locus_tag="JNC24_001359"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HAY9158150.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            28700..30184
                     /gene="gppA"
                     /locus_tag="JNC24_001360"
     CDS             28700..30184
                     /gene="gppA"
                     /locus_tag="JNC24_001360"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HAY9158151.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENALSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIANAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVEKEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQESQWQSYVHWPLEVH"
     gene            complement(30231..32252)
                     /gene="rep"
                     /locus_tag="JNC24_001361"
     CDS             complement(30231..32252)
                     /gene="rep"
                     /locus_tag="JNC24_001361"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HAY9158152.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPSQAAASAIGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
BASE COUNT         8185 a         8782 c         8145 g         7347 t
ORIGIN      
        1 tcatcgcagg agaagacgta aaagcaagcg tttagcgact ctttatgaag tctgaaaagt
       61 gaggaaatgc tgcgagtata acgcttactc gtggggattt atgttttgct ggtagtttcg
      121 tgatcggcac ctcgcgccag gatggctcta tgcactatcg caatgtggtt tgtttgttgt
      181 cttttttagt tccgtcagcg tgaggatgct cgctcggtaa acctgatcat aatgtttacc
      241 agaaacaggg aaaggcgtgg tgtaactgcg ccctaatgaa gagacaaatc tttcgctaca
      301 cagagacatc attattccgg tgacggatga atcgtgccgg aacgcagccg cagaggtgca
      361 aactttgaag ccctgacccc atgccgctat actgccgcca tcacttaaaa acaggataca
      421 acaatgaacg acagtgaatt tcatcgcctg gctgatcaac tctggctgac cattgaagaa
      481 cgcctggacg actgggatgg cgacagcgat atcgactgcg aaatcaacgg cggcgtgctg
      541 accattacct ttgaaaacgg cagcaaaatc attatcaacc gccaggaacc gctgcaccag
      601 gtatggctgg caaccaaaca aggcggctac cattttgatc tgaaaggcga tgagtggatt
      661 tgcgatcgca gcggcgaaac cttctgggat ttgctggaac aggcggcgac gcagcaggcg
      721 ggtgaaacag tcagtttccg ctaagattgc atgccggata agcctcgctt tccggcacgt
      781 tcatcacgaa aaatattgct gtaatagcgg cgtatcgtga tcctgattgg caggcggcat
      841 gttaccgata gattttgtgc ggaacggaat cacctgttca cgaccatcaa ccttcacaat
      901 ctgatagaac tgcggcaggt tgaagttgat gaagcttgag ccgtaggtga agcggtcgtg
      961 cgatgacgag tagaagcgac tgacgtcacg caccagttcc tctttgctgc cttcgcagtg
     1021 gtgatacacc tcaacccggt tgctttcgtc aagaatgtag atattaaagc cattctcgtc
     1081 ttgcgtttct tcgaagaaga actggatgat cccttcacta gcaaagccgt ccaccaccgc
     1141 cggtaatttg acgtgattgg tttcaacctg cactgacagg ccgtgcagtt tgttatgcga
     1201 aatagcgcca taaaactcga tggcgttctc cagcttctgt accgatacat tcaggcgttc
     1261 gaagaacaag ccccaggttt gaccagaaac gcgcagcgcc ttgaaacgcc cggtttcctg
     1321 gcgggtgctg gaaaggcgca gttcaataca ctcagaaacc agttgctgca cgcgagtacg
     1381 gattaacccg cgcagatgct ggctataaca gaagacttcc acgctatctg gcggtgcggc
     1441 gtcctgatgc attttgccga gaatagtttt cagggcttcg atcatcgatt gctcgccgtt
     1501 gaagtgcagc gtacgcactt cgttccacga gttgcggtac agcaggtcaa cgctacctac
     1561 caggcaattt tgattctcgc caaagctgaa gacatccagc ttacggaaat caaaatgcac
     1621 cacctgattg cggaacgccg ctgtcgggtc atattccagg ttaacgataa tcgccagatg
     1681 gcggatctca cacgggctgt agagcgcctt cggtgtcggt gcaggtaagc gcagcgggaa
     1741 atggtgcgac acgtcggcga ccatctcctg caacttaggc aaatcgacaa tgccgttacc
     1801 tttaatatac aaacgggtgc gcgaggtcag cagaccgtta aaccatgccc acgccaccag
     1861 tttattcagg taacggttat attccagcgg ctgatggctg atgatcgact caatatttgg
     1921 cgcgcggtta tacagatacc aacctgaacg gttagcccgg cccggcggca cataaataaa
     1981 ggtcagattc ggttccgaga gatcgggtga aatctgcggg tttaccagcg tcactttacc
     2041 tggtaatgct tcaaacgcgg catacagctt acgcgtcagc acgccgatat cctgcggact
     2101 ggcggagacg ctaaggttat tgcgacgcgc aaagcggatc agattacggt agctctgcat
     2161 catcgcgtcg agcaactcgt tgtgcgcctc acgcacctga tcaatcttcc agttagcacg
     2221 gttatcgagc attgccagac gagcttcgtc ccaaccccac tcgctcacta actggctcaa
     2281 cactgcgcga cgccagccta cacaggcgcg ttcacggctg agtttttcgc acacttttaa
     2341 atagaagcag cgacgtacta aatccagacg ggtgaaatct tcaatcgccg tcaggtattc
     2401 agtaacacgc tccagcatca tgcagtatgg atcgaggcca aacgatacaa tctcgccgtc
     2461 gtgcaaacgc tgtttgatat ctttcgccag cagacgtggg ttcgggtatt cccaggaata
     2521 ggcttccagc agcagtgttt tcagcaccgc tttgtatggg gaatcgatac tcttgtagag
     2581 ctgccaaagg ctggcaccaa agtactcttc agcagaaagc gagcttaagc caccgagatc
     2641 cagccattca tttggcgtca gcacgccctg cgcgtaaagc gtcatcacat agtcgtcgta
     2701 atgctcttct tcgtcgcacg gcaccatatt ccacagaata cgcttaccgg cgagacgcac
     2761 ggcggtacga taaaattcgt caagcagcag tatatgctgg gtggagccac aatcttcgcc
     2821 ccccaggctg ccgctttcat tatgacgaaa gcggttttca tcaatcagga agaagctgac
     2881 ttccacaccc agcgaggcgg cccagctttc cagcaggctg catttacgtt gtagtaactg
     2941 gcgctcttcg ctatcgagcc aggattgatg acagacccag atatccaggt cagaggaaca
     3001 actttgccct acggacgagg tgctgcccat ggtgtataca ccagtaattg gaagctcacc
     3061 tttcggtgga tcctgtactg acattccacg atacagttca agctcgttca ggtagtggcg
     3121 ttgagtttca tcaggcgtgt aaaggcaaat gcctttggga acgttaccat caaggtaacc
     3181 cggcattagc ggatggtgat agtgcaacaa tgtcggcagt agactgtaga cctgttggaa
     3241 tgcaggcccc atagcagcaa gcgcgcgatc cacacgcaat tgatttatgg catccagtct
     3301 ctgtttcaga gtctcaatat agaggtacaa gacgtatcgc ctgatttgct acccgtcatg
     3361 actgtgattc cgccaacatc aacggtaaca cgcggcattc gggatatttc gtatgtcaaa
     3421 ggtaaccgtt accacttttc gcgcctggtt tttttagttt cacgacgaaa aaatggtcta
     3481 aaacgtgatc aatttaacac cttgctgatt gaccgtaaag aaagatgcgc tacatacaag
     3541 tgtagcaccg tttattctct gtaaattcct tattacgaca gcgtgaaacg tctgtcagga
     3601 tccactgcca gacctcattt tacggtttgc gcaggcgtct acgtttcacc acaacactga
     3661 catcactctg gcaaggatgt taggatggac cacggatgat aatgacggta acaagcatgt
     3721 tagacaatgt tttaagaatt gccacacgcc aaagcccact tgcactctgg caggcacact
     3781 atgtcaaaga caagttgatg gcgagccatc cgggcctggt cgttgaactg gtaccgatgg
     3841 tgacgcgcgg cgatgtgatt cttgatacgc cgctggcgaa agtaggcgga aaaggcttat
     3901 ttgtaaaaga gctggaagtc gcgctcctcg aaaatcgcgc cgatatcgcc gtacattcaa
     3961 tgaaagatgt gccggttgaa ttcccgcaag gtctgggact ggtcactatt tgtgagcgtg
     4021 aagatcctcg cgatgccttt gtgtccaata actatgacag tctggatgcg ttaccagcag
     4081 gcagtatcgt cgggacgtcc agtttacgtc gccagtgcca actggctgaa cgccgcccgg
     4141 atctgattat ccgctccctg cgcggcaacg tcggcactcg cctgagcaaa ctagataacg
     4201 gcgaatacga tgccatcatt cttgccgtag ccggactaaa acgtttaggt ctggagtccc
     4261 gcattcgcgc cgcgttacca cccgagattt ctcttccggc ggtaggacaa ggtgcagtcg
     4321 gtattgaatg ccgccttgat gatacgcgca ctcgcgagct gcttgccgcg ctgaatcacc
     4381 acgaaactgc actgcgcgtt accgcagaac gcgccatgaa tacccgtctc gaaggcggat
     4441 gtcaggtgcc aattggtagc tacgccgagc ttattgatgg cgaaatctgg ctgcgtgcgc
     4501 tggtcggcgc gccggacggt tcgcagatta ttcgcggtga acgccgcggt gcgccgcaaa
     4561 atgccaaaca aatggggatt tcgctggcag aagagctact gaataacggc gcgcgcgaga
     4621 tcctcgctga agtctataac ggagacgccc cggcatgagt atccttgtca cccgcccgtc
     4681 tcccgctgga gaagagttag tgagccgtct gcgcacactg gggcaggtgg cctggcattt
     4741 tccgctgatt gagttttctc cgggtcaaca attaccgcaa cttgctgatc aactggcagc
     4801 gctgggggag agcgatctgt tgtttgccct ctcgcaacac gcggttgctt ttgcccaatc
     4861 acagctacat cagcaagatc gtaaatggcc ccgactacct aattatttcg ccattggacg
     4921 caccaccgca ctggcactac ataccgtaag cggacagaag attctctacc cgcaggatcg
     4981 ggaaatcagc gaagtcttgc tacaattacc tgaattacaa aatattgcgg gcaaacgtgc
     5041 gctgatatta cgtggcaatg gcggtcgtga gctaattggg gataccctga cggcgcgcgg
     5101 tgctgaggtc actttttgtg aatgttatca acgatgcgca atccattacg atggtgcaga
     5161 agaagcgatg cgctggcaat cccgcgaggt gacgacggtc gttgttacca gcggtgaaat
     5221 gttgcagcaa ctctggtcgc tgatcccaca atggtatcgt gagcactggt tactacactg
     5281 tcgactattg gtcgtcagtg agcgtttggc gaaactcgcc cgggaactgg gctggcaaga
     5341 cattaaggtc gccgataacg ctgccaacga tgcgctttta cgggcattac aataactctc
     5401 ataacaggaa gccataatga cggaacaaga aaaaacctcc gccgtggttg aagagactag
     5461 ggaggccgtg gacaccacgt cacaacctgt cgcaacagaa aaaaagagta agaacaatac
     5521 cgcattgatt ctcagcgcgg cggctatcgc tattgctctg gcggcgggcg tcggtttgta
     5581 tggctggggt aaacaacagg ccgtcaatca gaccgccacc agcgatgccc tggctaacca
     5641 actgacggca ttgcaaaaag cccaggagag ccaaaaagcc gaactggaag gcattattaa
     5701 gcaacaggct acgcagcttg aacaggcgaa tcgtcagcaa gaaacgctgg caaaacagct
     5761 ggatgaagtc cagcaaaagg tcgccaccat ttccggcagc gatgctaaaa cctggctgct
     5821 ggcccaggcc gattttctgg tgaaactcgc cggacggaag ctgtggagcg atcaggacgt
     5881 cacgaccgct gcagcgttgc tgaaaagtgc agacgccagc ctggcggata tgaatgaccc
     5941 gagtctgatt accgttcgtc gggcaattac cgatgatatc gccagccttt ctgcagtatc
     6001 gcaggtggat tatgacggca tcatccttaa gcttaatcag ctttcaaatc aggtagataa
     6061 cctgcgtctg gccgataatg acagcgatgg ttcgccgatg gattcagacg gtgaagagct
     6121 ttccagttcc atcagcgaat ggcgtatcaa tctgcaaaaa agctggcaga actttatgga
     6181 caacttcatt acgattcgcc gtcgtgatga caccgccgta ccgctgttag cgccaaatca
     6241 ggatatctat ctgcgcgaaa atattcgctc tcgcctgctg gtcgcagcac aagctgtacc
     6301 gcgtcatcag gaagagactt atcgccaggc gctggagaac gtctccacct gggtacgtgc
     6361 ttactacgat actgatgatg ccaccaccaa agcgttcctc gacgaggtgg accagttaag
     6421 ccagcaaaat atctcgatgg atcttccgga aaccctgcaa agccaggcga tgctggaaaa
     6481 actgatgcag acccgcgtgc gtaacctgct ggcacaaccg gcagcgggga caacggaagc
     6541 taaacctgca cctgcacctg cacctgcacc tgcacctgca cctgcacctg caccgcaagg
     6601 tgatactccg gcagccgcgc cgcaaggaga ataaccatgc taaaagtgtt attactcttt
     6661 gttttgctga ttgcggggat agtggttggc ccgatgattg ccggtcatca gggttacgtg
     6721 ctgatccaga ctgacaacta caatatcgaa accagcgtca cgggcctggc gattattttg
     6781 attctggcga tggtagtgct gtttgccatt gagtggctac tgcggcggat cttccgcaca
     6841 ggcgcacaca cccgtgggtg gtttgtcgga cgtaagcgtc gccgtgcccg taagcagacc
     6901 gaacaggcgc tgctgaaact ggcggaaggc gattatcagc aagttgaaaa gctgatggcg
     6961 aaaaatgccg atcacgcgga acaaccggtg gtgaactatc tactggctgc cgaagccgcg
     7021 caacaacgtg gtgatgaagc acgcgccaac caacatctgg aacgcgcagc agagctggcc
     7081 ggcaatgaca ccattccggt agaaatcacc cgtgtacgtc tgcaactggc ccgtaatgaa
     7141 aaccatgctg cacgccacgg cgtggataag ctgctggaag ttacgccacg ccatccggaa
     7201 gtattacgtc tggcggaaca ggcgtatatc cgcacaggtg catggagttc tctgctggat
     7261 atcatcccat caatggcgaa agcccatgtt ggtgatgaag aacatcgtgc aatgctggaa
     7321 caacaggcat ggattggcct gatggatcag gcgcgtgccg ataacggtag tgaaggtttg
     7381 cgtaactggt ggaaaaacca aagccggaaa acgcgtcatc aggtagcgtt gcaggtggca
     7441 atggcggaac atcttattga atgtgacgat catgatactg cccagcaaat tatcatcgat
     7501 ggcctgaaac gccagtacga cgatcgccta ctgctgccga ttcctcgact gaaaaccaac
     7561 aatccggaac agctggaaaa agtgctgcgc cagcagatca aaaacgtcgg cgatcgcccg
     7621 ctgttgtgga gcacactggg ccagtcactg atgaagcacg gagaatggca ggaagcatcg
     7681 ctcgccttcc gcgcagcgct gaaacaacgt ccggacgcct acgattacgc atggcttgcc
     7741 gacgcgctgg acagactgca caagccggaa gaagctgcag cgatgcgtcg cgacggtttg
     7801 atgttaacgt tgcagaacaa cccaccacag tagttccttc tcacccggag gcaagcacct
     7861 ccgggacctt tctgatgcat aaaaaaacgc ctgctcttat tacggagcag gcgttaaaac
     7921 aggtctgtat gacaacagtg ggtgcttcac tcaacgttgt gtccatggtg tctgatgagg
     7981 cataagcgac atctgtcagt ggacgataag caccgtaaac ggctctgcgt cattccggag
     8041 tttatgaggc actaaggcga acataagaga tggaatgagc atctacccgt ttattatgcc
     8101 acagagaatc gggaaataac atcccttaac acttgttatg agataattct gtaatcctct
     8161 ttgcttcctg agtgaatatt taacctgagc ttgatcctac acatacttat tatgaatgat
     8221 aaaattcatt caattaataa cacatatatt aattgccgtt aaaactaaaa aagcattaat
     8281 aatcaacgca atataataaa cccgccttac atatcaactg cgccagaggt aggattgaaa
     8341 acgctctcct gattttccaa ttcattttct ggataaataa ataatttatt tttgtcacta
     8401 ttatttatgt aatcatcctg tcagggagag ggatctcaat tatcaatgct taattacgtc
     8461 atcattttga tgacatgaaa ccggagcatt ttgcatggaa ttttcgtttt cacccaaact
     8521 tcttgttgtt gctgtcgccg ccgctcttcc tctcatggcc agcgcagcag ataccccgtc
     8581 aactgccacc gcacgcaaag gctttgccgg atacgatcac ccaaaccagt atccacagaa
     8641 cgctgcaatt tattgaattt gcacgatcat gtaggccgga taaggcgttt acgccgcatc
     8701 cggcaatcaa ccgcaggcgg ccacaaaatg gccgcttttt tacttattca ccaccagcaa
     8761 atgtgcctgc ataatgtcac tggccgggcg accgtgcgcc agcaaatcag ccattgcttt
     8821 aagatatggc ggtagatggc ggaaataacg ctgatacccg gcacacaaat aattcagccc
     8881 cggtttgccg ctggcatcga gcatgaagcg gtgtttcggg cagcctcccc agcacgcttt
     8941 taacacgtta caactgcgac actgcgccgg taattgctta aatttatctt caccaaacac
     9001 ctgctgttgc ggggaatcga tcatttctgc aattgtttgc tgatgcatat tccccagccg
     9061 atattgcgga taaacatagt gatcgcaggc gtaaacgtcg ccgttgtgct caacaatcac
     9121 cgagcgccca caggttggct gatgatggca aaccgcaccc ggcgcaccga caaaattggc
     9181 aaacgcccat tcgatattca tcacgaaaat cttgccgacg tcgcgtttga tccagtggtc
     9241 gaatatcgcc accagaaatt caccaaattc atcggggcgc accgaccatt ccgtcagttc
     9301 cccctgaata tcaccaggcg catgcaattt cagtccatcg cgggcagctg tttcatcagc
     9361 cagacgctcg accaccggaa taaactggat gaattcgact cccgcatcgc acaaaaaatc
     9421 ataaacctgc aacggttgct gcgcgctggt gcggttgacg cagaccagca cgttatagtc
     9481 gacatgatgt ttttgcagca gcgtcagggc acgcatcacc agcttatgcg ttggtctgcc
     9541 acctttggtc acgcgatatt gattgtggat ctcagccgga ccgtccagcg ataacccaac
     9601 aagaaaatga ttttctgcca gaaatgcaca ccatttatca tcgagcagca cgccgttagt
     9661 ctggaagctg ttacttatct tcctgccagc accgtatttc gcctgtaact ttacggcacg
     9721 gcggtaaaaa tccagcccga gtaacgttgg ttcgccgccc tgccaggtaa aagccacttc
     9781 gttttgcggt tcgctggcag cgatatagtg acggacatac gcttccagcg tgtcatcgtc
     9841 catatgcgtg actggctttt cgcggtaaag ggattgtttt tcgagataaa aacagtagtc
     9901 acagttcaga ttacaatcgg aaccactcgg tttcgccatc acatgaaaag cacgcgttgg
     9961 aacctgttgc agcatggtca ctccttgcct gaagtcatcc ttcaatacgt taagggcgaa
    10021 atcataccgt ggggcaatga gttattacgg gcgtttattg cgaagggagg gtgacgaaat
    10081 gcacagaaaa caaaaaaccc cgccgaagcg gggttcaaaa ttggtcggcg agagaggatt
    10141 cgaacctccg acccactggt cccaaaccag ttgcgctacc aagctgcgct actcgccgaa
    10201 atactgcttt ttgaattttt agttcaattc tttaaagtcg tggtgcgagg ggggggactt
    10261 gaacccccac gtccgtaagg acactaacac ctgaagctag cgcgtctacc aattccgcca
    10321 ccttcgcatt gtcacaactt ctaataatgg ggtggctaat gggattcgaa cccacgacaa
    10381 ctggaatcac aatccagggc tctaccaact gagctatagc caccactaca aatcttgtta
    10441 cgcggtatta ctaccaccgc agctcaagcg ccgggactaa atggcgcgcc cgacaggatt
    10501 cgaacctgag acctctgcct ccggagggca gcgctctatc cagctgagct acgggcgctt
    10561 agcgccgttg cggggggata atacggactt cccacccctc tgtctagtcc ctttttaaat
    10621 aaattgcgcg tttggttacg gtttgtgcgt tttgctgctt attcctccag tttatgcgct
    10681 ttcccgtggc gattaaggcc aaaaacttta taaatcgccg tcaccgccag cataaagatg
    10741 atgccaacaa acagcgacat acgcgtatct tcattaaagt acatgccgat caaaacgcaa
    10801 atcaggaatg ccattgttac gtagttggcc cacgggaaca ggattgagcg gaacggatgg
    10861 ctggcaatcg ccgctttatg tgcacgccga aaacgcagct ggcttatcaa tatcacaaac
    10921 catggcacca tccccggaag cacgctggca ctgtagacgt agacaaacac acgctgcgga
    10981 ttgggaatga tgtagttcag gcatgagcca attagcagaa tagcaataga tactgccaca
    11041 cccgcaactg gtacgccgtg acgggaaact ttcgccatcg ccgccggtaa ctgacggttt
    11101 ttcgccagtg cgtagagcat acgtccgcaa ctgtacatgc cgctgttaca gccagagagc
    11161 gcagccgtca gcaccacaaa gttgataatg cccgccgctg cggtaatacc gattttggca
    11221 aaagtcagta cgaacgggct gccgttgctg ccaatttcat tccacgggaa gatggtgacg
    11281 ataacgaaaa tcgcgcctac gtagaaaatc aggatccgcc acagcacttt gcctacagca
    11341 ctgcgcagcg tcacctgcgg attcttcgct tcaccggcag taatgccaat cagctccacg
    11401 ccctggtagg acgccaccac aatacacagc gcggtcagga accctttcca gccacccgca
    11461 aagaaaccgc catgctctgt gagattgcta aaaccaatcg actgcccgcc attgccaaag
    11521 ccaaagaaaa tcacgcccag gccaatgaca atcatcacga taatcgtggt gactttgatc
    11581 atcgcgaacc agaactcgat ttcgccgtac aaccgcaccg ccgccagatt cgccaacgcc
    11641 accagcgcca ccgcgatcaa tgcaggtatc cactgcgcca tctccgggaa ccagaactgg
    11701 acataaacgc caatggcggt gatttctgag atccccaccg ccatccacat aaaccagtaa
    11761 gaccaggcgg tgagataacc aaaaaacggg ctcatataac gatgcgcata aacggcgaac
    11821 gaaccggtaa caggttcaag gaacaacatt tcgcccattg aacgcatgat gaaaaagacg
    11881 aacagcccgg cgatgatata ggccaacaat acggatggcc cggcccattt cagggtactg
    11941 gcggccccca taaacaggcc gacgccaata gtgcccccca gggcgatgag ttcgatatgt
    12001 cgagcttcca gcccacgctg tagctctggt ttgttatctg ccataaatcc tcgtgttgtg
    12061 tttgcatgct ttccggtgtt accggttatc gttatgggta catcgagtgt tgcgaatgtt
    12121 ttcgtaattc aggagaaatg gcaaataaag cattaaaaat ttgaatgctt tgtgtaataa
    12181 aaaagcagac aggcgacgga gtgaccactc cgtcgcttta caaagagagg aaaatcagag
    12241 gttgccggtg tagtgccagc gtaaataacg cagcaaacga agctgacgct taatgcggct
    12301 cggctgcgaa agcaggcggt agagccactc cagtcccagc gtttgccaga ttttcggtgc
    12361 gcgttttacg tgaccggtga aaacatcgta agtcccgcca acgcccatat acagcgcatc
    12421 tggatgcacc agacggcagt cgcgcatgaa gatctcctgc tttggcgatc ccatcgcaac
    12481 ggtgacgatt tgcgctccgc tggcatgaat gcgttcaaac agcgcctgac gctgctcggg
    12541 tttaaaataa ccatcctgac tgccaacgat attcacattc cattggttgc gcagtttagc
    12601 ttcagtttgc gccagcactt caggtttacc gcccacaaga aataccggcg tcccttcttt
    12661 gcctgcgcgc gccatcagct cttcccagag atcggcaccg gcaacgcggg aaacctgcgc
    12721 ctgcgggtac tttttacgta ctgaacgtac aacgctgatg ccatccgcat atttaaattc
    12781 ggcagcgtta attaactccc tgacctcggc gttatcttca atggtcagca ttttttcagc
    12841 attaatggca accagcgttc cctgcttaag ctgcccgtca gcaaacagat aatcgagggc
    12901 gtgctgcata tcacgccaac caatcaactg taagccacgc agcgtatagg tcggtgccgt
    12961 ggtgttgtta ttcattgtta tccttcaacc tgcgtccgga gcgatgattt tgtacgttta
    13021 tgaatgagtc cggcgctttc aaaaagccag tacagcagtt ttgcgatcat cagacatgcg
    13081 ccgaagacca cgataaaaaa gactacgcgt gagacaaacg aatccagccc ttcacgcgcc
    13141 agcacgatca tattgaagat cgccccaaag cagaaactgt gcaatatcgc agccttatag
    13201 cgattaggct cgcggttgcc cagctcatac agccagtcga accatttgat gatcagacca
    13261 accacgatcg ccccgagcgg gatgaacaac gcgccgccca tcaccaccag tgagcctata
    13321 agcgtaggcg agatcgccag tccggagtgg ttattcagca cttcccaggt aaagtagttg
    13381 gctgagttca gcaccatact cgggcgaccc ggccacagcc aggaagggat aaagacatag
    13441 aaatcgcgga caattggagc caggccctgg aagtcgatgt tgtcgtagtt ctgcaacagc
    13501 aacgccagat tctcccacgg ggagaaggtg tcgcgagtga gatagagaaa cgtatagaac
    13561 gcttcatcgc cgctcacatt cattccatag cgttttagcg ccagccagaa catgccaaca
    13621 atccccagca cgcccgccgc cgccagcatc cacaacgaaa tccagccgcg aataatgcca
    13681 ataaacagga agatagcgaa tgcgatgatg atattggcgc gagtgccgcc gacaatcata
    13741 taagtcagca agccaaaggc gaccgtgctg acgaggaaaa acagccacgc tttgctgtcc
    13801 tggcgcagaa agtagaccac cagcatcgcc gggatgaaaa agtaaaagaa gcgttttaac
    13861 gccacgccgg agacttcact ggagaagatc tgactgtagg agttaagccg gaacagcaaa
    13921 aagccgttgt gcatgaagaa gatgccgacg cttaccagcg cgatacccat caggatcacc
    13981 cacgtaagat tggtctccac gcggttcatg gtaaacagcg gacggcgcgg tacatcagca
    14041 acgcgtttgc gtaggcgggt tttgtaggtg acatagtaaa ccgcgtagaa gcagcccgca
    14101 gaaagcaacg cctgcaacaa gatttctggc ggcgcgacac caacatcaaa gcgaaatacc
    14161 agcacgctgg tcagcgggaa gccgaagaaa aaggtgagca aaaacagcaa tgaaaagaag
    14221 acattgaagt taaagcgcac acggcgaaac tcaaaccagg tcagcgtggc aataaacagc
    14281 gtgcagagca gccagaccac aaacaggcca ctgaattgca gcagactcat gcgacctccc
    14341 cggcggcaat agccaacgcc cgctgccagc cttgtagata gttagggcta aagaaggcaa
    14401 tggtgttttt atccaccgac gccaactggc gctgcgcttc acgcacaata tcctcgttga
    14461 gatcgtcggt agtaaacagc accggcaaat gctgttccgt catatcctgc cagaacggat
    14521 tttcccggtt aagcacacaa ggaatgcccg cctgaatcag taagcaaagc gtaccaatgc
    14581 cctgctggcg ggcaaaaata aagtaaccaa gatcgcactg acgaagtagc gccagatagg
    14641 cgtcaaattc cagtttttcg ctcaaaattt gcagattttc ttcgctgaat aactccagcc
    14701 ccgcctgacg aacttcctca atgtacgctt cgttattagg cggatatccc atcggcacca
    14761 ccacttttac cgtatcgcca aattgctgat gaacggcgcg caaggcagca atatggtcat
    14821 tgctgcggtc accggagttc cccaccagaa tggtcatttt cccttcacgt tgccgatcat
    14881 tcgccatcgt attgagagaa gggtccatcc gcgtcgggaa gtacagcagt tcgccccgca
    14941 cctttggatg cgttttggca aaaaagctca aatcaccgcg ggtggcaaat acacagccga
    15001 ctcgcttttg cgccaggcga cgtagcgggt aaaaaagctt atatctcaag ccactggaaa
    15061 gctcatacag gtctgccccc cagatatgcc agaaaaactg gctgggctta atgccaccac
    15121 tcagcagagc cagccacagt gtgggattga actgaccgtg gaaaaaaaat cgctgctgac
    15181 ggttagcttt tgctttcgcg atgaccgctt ccgccagcga ttttttccca gggaaaaatt
    15241 gcacagaaag cgccggacag ctatcactta agccgtcgtc cttgccaaca accataaact
    15301 cgcgcgcgtg ctcgctcgtc gcggccagcg cgtcattgaa aaaccgcaaa acggttcggt
    15361 tatggtgagg gatatccgat cccagtacgt gaatcagtac agtcatgccc gcctacgcca
    15421 gagtaaaaac acgccacaac aaagagaaaa atagacgata taagttgcca tatatgcctg
    15481 cgccgcgccc agtgcaccat gcgcagggat tagccagtgg gcaaatacca tcaataaagt
    15541 gaactggctg acttccgcca gaatataaaa ccgcagtgac gctttggcga tcaccagata
    15601 accaaagaca taagcgccca cttttaacac atcacccact aactgccagg caaagagatc
    15661 gcgcatagcg gtaaatttat tcgacaacag caaccagata gcaaaatcac gcagcaacca
    15721 gacggtaaaa ctcgccgccg ccactgccgg taagacgaat ttcagcgatt taaccacttc
    15781 ccgggtgata tcgcgctttt ccgttagccg cgacaacgtg ggcagcaaat atacgctgaa
    15841 cgatgccgta ataaattgca ggtaggcatc ggaaatactg ctcacccctt gccagatccc
    15901 cacttcatcc cagctatact gcgccgccag cagtttacgc atcatgatgt aagcaacagg
    15961 caaggtcacc gacgtaatca acgccatgag cgtaaatttg ctcaactgcc ctgccagacc
    16021 gttatcccag ctgggtttca gatagcttaa cgggatgaca ccacgtttga tcaacatgat
    16081 ggcggcagga atcaccacca gcgcgggaat cagcgccaga cccagcaacg ccccttcata
    16141 accgcccaaa cggtaactga cgtagtacgc gagaacgcca atcaagctgc cgacaatcag
    16201 cgataacgca ttacctgcgg catcgcgaaa gcctttcatc agcgccagta acaggttgcc
    16261 ccaggcgatc cccatttgca ccagcgccac taaacgcacc agcccctgat agtcggtatt
    16321 accaaacagt ccctggctga ttggcgcagc tgccagcaca aaaaccagcg ccatcagcgt
    16381 agagaaacca agtaccatcg ctgatgaagt gccgaccacg cggcgcagct gttgcggatt
    16441 atcatggtac tgggcaacgt atttggttac accgttaaag atgccagctc cggcaagcac
    16501 gccgagcacg gtaatcaact ggcggaaatt tgccgccagc ccaagccccg ccggaccaaa
    16561 tgacacagcc agcaacttac cgaccagtaa cccggcacca atcttgacca gtgtactggc
    16621 cgccgtccac aaggacgctt ttgccaacga catatcagga gaagtagttc aacaaagtcg
    16681 caattaccgt acgctgattg acgggcgaca ggttgtagaa cagcggcagg cgcagcaggc
    16741 gctcgctctc tttggtggtg tagcgatctt caccgtggaa ctcaccaaag cgttcccccg
    16801 cagggcaacc gtgcagcgga atgtaatgga acaccgccat gatttccgct tctttcagaa
    16861 agttaatcaa cgcgctccgg tcatcaatat cccgcagttt aatgtagaac atatgcgcgt
    16921 tctgcacgca gccatcggga atcgacggca gctcgatacg cccggctttc gccagaggcg
    16981 ctaacgcatc gtagtagttt tgccacagcg ccagacgttg ctggttgata cgatccgctg
    17041 cttccagttg cgcccacagg tacgcagctt gcagatcgga catcaaatag ctggagccaa
    17101 tatcgcgcca ggtatattta tcgacctgac cacggaagaa ctggctgcgg ttagtgccct
    17161 tttcacggat gatctcggct cgttcgatta acgctttatc gttaatcagc gtcgcgccgc
    17221 cttcaccacc cgccgtatag tttttggttt catggaagct aaagcagcca atatgaccaa
    17281 tggttcccag tgcacgccct ttgtaagtgg acatcacgcc ctgagcggca tcttctacca
    17341 caaacagatt atgctttttc gccaacgcca taatggtgtc catttcgcag gccacacccg
    17401 cgtaatggac cggcacgata acgcgcgttt tgtcggtgat cgccgcttca atcagcgttt
    17461 cgtcgatgtt catggtgtcc gggcgaacat ccacaaaaac gatttttgcg ccacgcagca
    17521 caaaggcatt ggcggtggag acaaaggtgt agctcggcat gatcacttca tcgccaggct
    17581 ggatatcgag cagcagcgcc gccatctcca gcgaagcggt gcaggacggc gtcagtaaca
    17641 ctttggcgct gccaaaacgt tgctccagcc actgctggca gcgacgggta aaaccgccat
    17701 cgccacacag tttgccgcta cccattgccg actgcatata gtcgagttcg gttcccacca
    17761 ccggcggtgc gttaaatgga atcatgtgat cacctgtata accagtacgc ggtgctttct
    17821 acattcgcac cactttgtat gtatcgttta agcgcggcgg tgttgcccat ttgggtcgcc
    17881 acccgcaaag ttgttttacc gcgagcatac gcccagttta gcgccgtttg catcagctca
    17941 gcacctgcac cgcgtccagc cagcaggcca attcgcgcat ctgtcgcatt gagttcccgt
    18001 aaagagacat aaccgcgaat accgccggac gccgcacgta aaatcagaca ttgatgatca
    18061 aaggtgccgc gcacggcatt ttcaatccac tgtgcataaa agcgtccgct ggcgtcaggc
    18121 gcataccacg gcgcacgaaa acggctttgc gcaaatgcgg cgctggctaa ctgacgtaat
    18181 gcgggaatat cggtctcttg tgccactaca gcaccactat cactgacatt gttcacgggt
    18241 agtgccaaat caacttcacc ttctaccagg gagaatccca gctgttgcag ggcatccagt
    18301 tcacccgtat ttgatgccgc aattttggcc tgcacccgtg accacggcgc taacacgtct
    18361 ggcgtcagga gctgtgcttc agacgtaatg cgcacgatgg cgctgttaac accaaagaag
    18421 gcgttttccc aggttagtgg ctcaatactg gcgcggacgg gcacgaagta actccagcag
    18481 atattggccg tagccagttt tcgctaatga actggcagca cgcttcacac cctcgtcatc
    18541 gagccagccg ttacgccagg cgatctcttc caggcaggca atcttaaagc cctggcgttt
    18601 ttctactgtc tgcacaaagg tactggcttc aatcaggctg tcgtgagtgc cagtgtccag
    18661 ccaggcaaat ccgcgcccga gcagttcaac ggtcaggttg cccgcctcga ggtacatctg
    18721 gttgatggag gtaatctcca gttcaccacg ctccgacggc ttcacctgct ttgcgtactc
    18781 cacgacttta ctgtcgtaga aataaagccc ggtcaccgcc cagtttgact tcggctgttt
    18841 tggcttttct tccagcgaga tagcgcggaa attgtcgtca aactccacca cgccaaagcg
    18901 ttccgggtcc atcacctgat agccaaatac cgtcgcccct tccgtgcgcg ccgcaacatg
    18961 acgcagcttc ggactgaagc cctgaccgaa gaagatgtta tcgcccagca ccagacaaga
    19021 aggttcaccg ttgaggaagg tttcaccgat gataaaggcc tgcgccagac cgtccgggct
    19081 gggctgttcg gcgtattcca gctggatacc gaactcacta ccatcgccca gcaggcgctg
    19141 gaaataacct ttatcttccg gcgtagtgat gatgagaatt tcgcggatat cggccaacat
    19201 cagcaccgac agcggatagt aaatcattgg cttatcgtaa atcggcaaca gttgcttcga
    19261 tacgccgcgc gtaatcggat gcaatcgggt gccggaaccg cccgccagga taataccttt
    19321 catgtttgcc tccgctgaaa attagccttt cagacctaaa cgctcgccct gatagctgcc
    19381 gtcctgcacc tgcttccacc agctttcatt agccagatac cactgcaccg ttttacgcat
    19441 tccactttca aaggtttcct gtggcagcca gccaagttca cgggcaattt tcgaagcatc
    19501 aatggcataa cgcagatcat gccccggacg gtcagcgaca aaggtgatca agtcacgata
    19561 gtgcaccacg ccgtgcggct tgttcggagc cagttcttcc agcagctcgc aaatggtttc
    19621 cacaacatcg agattcttac gctcgttgtg accaccaata ttataggttt caccgacttt
    19681 cccggtggtc gccacgcaat acagcgcgcg ggcgtgatct tccacataca gccagtcacg
    19741 gatttgctgc ccgttgccat ataccggcag cggtttaccc gccagcgcgt tgaggatcat
    19801 cagcgggatc agtttttccg gaaagtggta aggaccgtag ttattcgagc agttggtgat
    19861 aagcgtcggc agaccgtagg tccgcagcca ggcgcgcacc agatggtcgc tgctggcttt
    19921 tgacgcggaa taagggctgc tcggcgcata cggcgtggtt tcggtgaaga aatcatccgt
    19981 cgagtgcagg tcaccatata cttcgtcggt ggagatatga tgaaaacgga acgctgattt
    20041 tttatcttcc gtcagcgtat tccagtaagc ccgcgccgct tcaagcaatg tataagtccc
    20101 gacaatgttg gtttcaataa acgctgccgg gccgtcaata gaacggtcaa catggctttc
    20161 ggctgccaga tgcatgacac agtctggctg atgctcagtg aatacgcgtg ccagttctgc
    20221 ccgatcgcag atatcaactt tctcaaaggc aaagcgctcg ctttgcgcga ccggtgccag
    20281 cgacatcagg tttccggcgt aggtcagctt atcgaccact accaccgcgt cgctcgtttc
    20341 gttgatgata taacgcacca gcgccgagcc aataaacccg gcaccacctg ttatcagaat
    20401 ttttctcatc agcgccagac tcctttggca tcgacgacat actgctgatg gacattgtcg
    20461 ccattgataa ctttaaactg actatgatcg accagcatca ccagcacgtc tgccgttgcc
    20521 agcacctcgt caagctgcgc cagagtacaa agcccggtca gttttttcgg caactggtgg
    20581 atgttaggct caacaaccag agtttcgccg ctatgccact gggcgatcag ttcagcgatt
    20641 tccatcgccg ggctttcgcg caggtcatca atattcggtt taaacgccag accaaagcag
    20701 gcgattttca gttcactggc gcgtttatcg gtagccgcca ggcaatcagc caccgccgct
    20761 ttcacctgat cgataaccca gaacggtttg tgatcgttca cttcgcgcgc ggtacggata
    20821 agccgcgcct gctgggggtt ctgtgccacg ataaaccacg gatcaacagc aatgcagtga
    20881 ccgcccacgc cagggccagg ctgaagaata ttaacgcgag ggtgacgatt cgccaggcga
    20941 atcagttccc agacgttaat cccctgatcg gcacaaatca gcgacaattc attagcaaaa
    21001 gcgatattca catcgcggaa gctgttttcg gtgagcttac acatttccgc cgtccgcgag
    21061 ttagtgacga cacactcacc ttcgaggaaa attttgtaca gttcgctggc gcgggtcgaa
    21121 caaaccggcg tcataccacc aatcacgcga tcgtttttaa tcagctcgac cataacctgg
    21181 cccggtaaca cgcgttccgg gcagtaagca atgttgacgt ccgcctgctc gcccacctgc
    21241 tgtgggaaag tgagatccgg acgcatctct gctaaccatt ctgccatctt ctcggttgac
    21301 cccaccggcg aggtggattc aaggatcacc agcgcgcctt ttttcagcac tggcgcaatg
    21361 gagcgagcag ccgattcaac gtaggtcata tctggctcat gatcgccttt aaacggcgtg
    21421 ggtacagcaa tcagccaggc atccgcttca actggcgtcg tgctcgctcg taaaaaaccg
    21481 ccttctacgg cagtttttac tacgctcgcc aaatcaggct cgacgatatg gatttcgcca
    21541 cgattgatgg tatcaaccgc gtgttggttg atatcgacac caatcacctg tttttgccgt
    21601 gaggcaaacg ctgctgctgt aggcagcccg atgtaaccca gtccgataac agaaatggtc
    21661 gcaaaactca tagtgatatc cgattatttt ttaacgcttc cagaatgcga gagcatgcct
    21721 gaccatcacc atacgggtta tgggcgcggc tcatagtttg atattcgttt tcgtctttta
    21781 aaagacgcgt cacttcctcg acaattcgct gcttatccgt gcctaccaga cgcaccgtac
    21841 ccgccgtcac cgcttccgga cgctcagtgg tatcgcgcat caccagcacc ggtttcccca
    21901 gcgaaggcgc ttcttcctga atgccgcctg agtcggtcaa aatcagccag gcgtggttca
    21961 tcagccagac aaacggtaaa tactcctggg gatcgatcag aatgacattt ttcacatgcc
    22021 ccagaatgcg attgaccggc tctctgacgt tcgggttgag atgcaccgga tagacaatct
    22081 ggatgtcctg gtgcgtggtg gcaatgtctg ccaacgcgtg gcagatttct tcaaagccac
    22141 gaccgaaact ttcacgcctg tgaccggtca ccagaatcat ctttttatcg gggtcgataa
    22201 acgggtaatt tgccgccagt tctgaacgca gcgtatcgct gctcatcacc tggtcacgca
    22261 cccataacag tgcatcaata actgtattac cagtaatgaa gattcggcta tccgcaacgt
    22321 tttcacgcag caagttttgc cgggaagttt cggttggaga gaagtgatac atcgccagat
    22381 gcccggtcaa tgtacggtta gcctcttccg gccacggcga ataaagatcg cccgtgcgca
    22441 gaccagcctc aacgtgacca acaggaatac gctgataaaa cgccgccagg ctggttgcca
    22501 gcgtcgtcgt tgtgtcgccg tgaaccagca cgacgtctgg tttgaactcg gcaagaatag
    22561 gttttagccc ttccagaatc cgacaggtta tctctgtcag gccctgtcct ggctgcatta
    22621 tgttgagatc gtagtcaggt acaatggaaa agagtttcag cacctgatcg agcatctccc
    22681 gatgctgcgc agtgacgcaa actttagcct caaaaaaagg atcttttgcc aacgcatgca
    22741 ccaacggcgc catcttgatg gcttccgggc gcgtaccaaa tacagtcagt actttcacat
    22801 cgattctctt cgaataagcg gcgagcgcct ttgcgctcac cgcagcagtg ttgctatttc
    22861 gagcaacggc gggttaatgc gacaccagcc ccgatcagcc ccccgacaat gccccacata
    22921 atcatcagga aggcacgacg tgggctatcg cgttttaccg gttcttccgg cgtacgcaaa
    22981 tagcgatagg tctgaaaacg cggatccagg gttggaccaa cattcagggt gtttaacatg
    23041 gcccgattct gatcatagtc gagatcaaag gccggaccga cggcctgtaa attttccagt
    23101 cgagcctgga gcattggacg cccaagcagg aacatttctg aatcaggtaa ttcctcggca
    23161 ggcacatctg tcgcactgcg cgaaatatta tgctgctcag caattttcag cgcctgttca
    23221 atactgttca tccggcggtc gtagatggct ttcgccacct cttcctgacg cttcacctgt
    23281 gctttcatct ggatggtacg cgccgcccat gcgcctttca gctcatcatt cagatggctg
    23341 gctgcacgct ggctggcaaa agcaacatac tgacgtaaca ggttattagc gtcaggcgcg
    23401 gtttcagcaa taagcttcac gctgtcattg accgcgcggg taaagtctcc ggggataaac
    23461 tggatgttgt taatcatttc atccagcaac gccgcatcgg ctttgctgtt gcccaccatc
    23521 cgctgtttgt aatagtcggt ttgcagccag aactctctgc gggtatccca cgaggccagt
    23581 tgcataacaa actctttata ggcttcgtcc atgaccgatg gttggtcggc agaagccatg
    23641 tttgaacgga catccaggtt acgcaaaaat tgctgctgcg agtaatatcc ccccagcata
    23701 ttcaccgttg gacgatcggt aatcgccgtc gagctccact cctgacgagc aaaaaaagta
    23761 tacgccagcg cgattaacgc aaatgccagc cccatgccaa taatccatag cttcccagcc
    23821 cacaaggtac gaaacaaccc acgaatatcc agttcatttt cagcgtcttc ggccggtttc
    23881 ccaggcattg gttgtgtcat cacatcctca tttatttggt taaattgggg ctgccaccac
    23941 gatttctacg cagtctgcgt tttacgcgct taataaagcg agcaactttc caggcacgct
    24001 taatgcaata tccatagagg aagaatgcta gcaaaaagag caccagcatg acccactccg
    24061 ggacaaaatg agaatattct gccagcacgc caatggaagc gagcagtgcc gcagcaaggg
    24121 taatcagcac aaacgcctga cgggaagtaa acccggcacg catgatcaaa tggtgaatat
    24181 gctgacggtc aggagagaat gggctcatgc ctttacgcag gcgacggtac ataatcgcca
    24241 ccatatccat tagcggaatg gcgattatcc acaaagcggt aaccgggctg atgggatggg
    24301 ttttgccctg ggtcgtttcg agcaggatcc agataacggt aaaaccaatc agcgtactgc
    24361 ccgcatcacc cataaagact ttgtagcggc gacccaggat accaaggtta agcatgatgt
    24421 atggcaggat ggcggcgatc atcgcaaagc accagattgc gaggctggtt tgcccgtcga
    24481 accacaaaat cataccgatt gctgcaaacg agacgcagga caacccgccc agcaagccat
    24541 caatgccatc aaccatgttg aacgcattaa tggccgccca gacggcaaat agcgtcagga
    24601 agtaaccaaa cggtccgagc accatctccc aggagccaaa gatataaccc aggctactga
    24661 gataaagttt gccgaacacc atcataacaa tgccaacagc ggcctgtatg gtggcacgga
    24721 tttttacgct gatatcaaaa cggtcatcca gcgcgccaat gaaaacaagc acaccggcac
    24781 aagcgagata gagagatgca tgcggaatat agtaatcgac aattccgaac gtgaagcaaa
    24841 tccctgcgta aaccgaaatc cccccaacga gaggtatcaa tccctggtga cgtttgcgga
    24901 agtttggttt atccactaaa ccgacttttt ttgccacctt acgggcaaaa aacagaaaca
    24961 gtgtcgtgaa taaaaaaata ctgatgagat cagtactcac tgtcagtaaa ttcacaatgc
    25021 atgctctcag agaaaattat tagcagaagt ataaccacga agacctttat tcagaaggga
    25081 aagtccgttc cgaacccacc agtcctgtgc aaaaattaac catttgatat gcaaattgca
    25141 taacacgcca gcaaattcca ggaattttcc taacatcgcc agcgcggcat taagattaca
    25201 gatataaaag caaaacgcca cgtaaacacg tggcgttttt ggcataagac aaatttatga
    25261 gcgtttcatc atttcgaaga aatcgtcatt ggtcttggtc atggccagtt tattaatgag
    25321 gaattccatt gcatcgattt cgcccatcgg gtgaatgatt ttgcgcagga tccacatttt
    25381 ctgcagttct tcctgagtag tgagcagttc ttctttacgg gtaccggaac ggttgtagtc
    25441 gatagccggg aagacgcgtt tttcagcgat cttacgagag aggtgcagtt ccatgttgcc
    25501 tgtaccttta aactcttcgt agataacttc gtccatttta gaaccggtat cgataagcgc
    25561 cgtcgcgata atagtcaggc tgccgccctc ttccacgtta cgcgccgcac caaagaagcg
    25621 tttcggacga tgcagggcgt tggcatccac accaccggtc aacactttac ctgacgccgg
    25681 aacaacggtg ttgtaagcgc gcgccagacg agtgatggag tcgagcagaa tgataacgtc
    25741 tttcttgtgc tcaaccaggc gtttggcctt ctcgatcacc atttccgcaa cctgaacgtg
    25801 gcgagatgcg ggttcgtcaa aggtagaagc aacaacttca ccttttacca gacgctgcat
    25861 ctcggttact tcttccggac gttcgtcgat cagcagaacc atcagcacac aatccgggtg
    25921 gttgtaagca atgctctggg caatgttctg cagcagcatg gttttaccgg ctttcggcgg
    25981 tgccacaatc agaccacgct gaccacgacc gataggtgat gccagatcca gtacgcgagc
    26041 agttaaatct tcagtagaac cgttaccacg ttccatacgc agacgagaat ttgcgtgcag
    26101 cggggttaag ttctcaaaga ggattttgtt gcgggcgttt tcaggtttgt cgaagttaac
    26161 ttcgttaact ttcagcagcg caaaatagcg ttcaccttct ttcggcgggc gaatcttacc
    26221 agagatggta tcaccagtgc ggaggttgaa acggcggatt tggctagggg aaacgtagat
    26281 gtcatcagga ccggcgaggt aggagctgtc tgcggaacgg aggaaaccaa atccatcctg
    26341 caatatctcc agtacgccat caccaaagat atcttcgcca ctctttgcgt gctgcttcag
    26401 gatggcaaaa ataatgtcct gcttacgcat gcgagccagg ttttccagcc ccatattttc
    26461 gccgagagtg atcagctcag aaaccggcgt attctttaat tcggtaagat tcatagtggt
    26521 gtgagttctt aaacttgggg taaatctcga acttaatgtt gtgaatggta tggcaggatc
    26581 atccatgcct gtttacggtc atcgactcat gtcttttcgc tgcctggtca caggagagta
    26641 cgcagaactg aaacgacaag acggattgag tgacgagcca gaaatctgtt cacttcgcat
    26701 ttaagataaa acgggaagcg ttggaaatta caagattcaa acttaataag gtatgtttaa
    26761 tacgaagtca acactaagtt agcatgactc acgccgggcg tccagttttt agcgacgggg
    26821 cacccgaaca tgaaattccc ttacgccagg ttagcgtcga ggaactcttt caactgacct
    26881 ttagacagtg cgcccacttt ggttgccgcc acttcaccgt ttttgaacag cagcagagtc
    26941 gggataccac ggatgccata tttcggcgca gtgccagggt tttgatcgat gttcagtttt
    27001 gcaacggtca gtttgccctg atattcgtca gcgatttcat ccagaatcgg ggcgatcatt
    27061 ttgcacggac cgcaccactc tgcccagaaa tcgacgagga tcgccccgtc cgctttgagt
    27121 acatccgtgt caaaactgtc gtcagtcagg tgaataattt tatcgctcat atataactcc
    27181 acaggaataa gcctggcgtg ttggtttcgt tgttggtgta acattaacca actaaaggtt
    27241 gactttattt caccggatac gctttcgtaa agcaatagta agctgatatt ctaccacact
    27301 atgagcaaaa cacatttaac agaacagaag ttttccgact tcgccctgca tccgaaggtt
    27361 gtagaagccc ttgaaaaaaa agggtttcat aactgtacgc ccattcaggc actggccctt
    27421 ccgctgacgc tggcgggtcg tgacgtagcc gggcaggcgc aaaccggtac cgggaaaacg
    27481 atggcgtttc ttacgtcaac gtttcattat cttctctctc atcctgcgat tgccgatcgc
    27541 aaggtgaatc aaccgcgtgc cttaattatg gcaccgacgc gtgaacttgc cgtgcagatc
    27601 catgccgacg cagaaccgct ggcggaagct actggcctga agctgggtct ggcttacggt
    27661 ggtgatggct acgacaaaca gctgaaagtg ctggaaagcg gcgttgacat tctgattggc
    27721 accacgggtc gtttaattga ctacgccaag cagaaccaca ttaacctcgg tgccattcag
    27781 gtagtggttc tggatgaagc cgatcgcatg tacgatctgg gctttattaa agatatccgc
    27841 tggctgttcc gccgtatgcc gcctgcaaac cagcgcctca acatgctgtt ctccgccacg
    27901 ctttcgtacc gggtacgtga actggcgttc gagcagatga acaatgccga atatattgaa
    27961 gtggaaccgg aacagaaaac gggccaccgt attaaagaag agcttttcta cccttctaac
    28021 gaagaaaaaa tgcgtttgct gcaaacgctg atcgaagaag agtggccaga cagagcgatt
    28081 attttcgcca ataccaaaca ccgttgtgaa gagatctggg gccacctggc ggcagatggt
    28141 catcgtgtcg gtttgttgac aggcgatgtc gcgcagaaaa aacgtctgcg tattcttgat
    28201 gaatttaccc gtggcgatct ggatattctg gttgccaccg acgttgccgc gcgtggtttg
    28261 catattccgg cagtgacgca cgtctttaac tacgatttac ccgatgactg tgaagattac
    28321 gttcaccgta ttggtcgtac aggtcgcgca ggcgcaagcg gtcactctat cagcctggcg
    28381 tgtgaagagt atgcattgaa tttgcctgct attgaaacct atattggtca ctcaattccg
    28441 gtaagcaaat acaatccgga cgcattgatg accgatctgc caaaaccgct gcgcctcacg
    28501 cgcccacgta caggcaatgg tccgcgtcgt actggcgctc cgcgtaatcg tcgtcgttca
    28561 ggttaaataa aatgtcatac tcaaaatcat tcaaactgta tcaagatggc aagagaatga
    28621 atcccgatga gcatactgta gtaagtgatt caggtgaaag aatgcagcca acacagagac
    28681 agattgaagg atgaagagta tgggttccac ctcgtcgctg tatgcagcca ttgatctcgg
    28741 ttcgaatagt tttcatatgc tggttgtgcg cgaggtggct ggaagcatcc agacgctgac
    28801 gcgaataaaa cgcaaagtgc gtctggctgc tggcctgaat agcgaaaatg ccctgtctaa
    28861 tgaagcaatg gagcgcggtt ggcaatgtct gcgcctgttt gctgaacgtc tgcaagatat
    28921 ccctccctcg caaattcgcg ttgtcgctac ggcgacatta cgtcttgccg tcaatgcggg
    28981 tgattttatt gccaatgcgc aggaaatcct cggttgtccg gtacaggtga tcagcggtga
    29041 agaggaagca cgtctgattt atcagggcgt tgctcacaca actggcggtg ccgatcagcg
    29101 cctggtggtg gatataggcg gtgccagtac tgaactggta accggcacgg gtgcacaaac
    29161 cacctcgttg ttcagcctgt cgatgggctg cgtcacctgg ctggaacgct attttgccga
    29221 tcgtaatctg gggcaggaaa attttgatgc tgcagaaaaa gcggcacgcg aagtgttacg
    29281 tccggttgcc gatgaattac ggtatcacgg ctggaaagtg tgcgttggtg cttccggcac
    29341 cgtgcaggcg ttacaggaaa tcatgatggc acaggggatg gatgaacgca ttaccctgga
    29401 aaagttgcag caattgaaac agcgagccat tcattgcggt cggctggagg aactggagat
    29461 tgacgggctg acgctggaac gtgcgttagt gttcccgagt ggtctggcga tcctgatcgc
    29521 catttttacc gaactgaata ttcagtgtat gaccctggcg ggcggtgcgc tgcgtgaagg
    29581 cctggtctac ggcatgttgc atctggccgt cgagcaggat attcgcagcc gtacgctgcg
    29641 taatattcag cgccgcttta tgatcgatat tgatcaggcg cagcgcgtag ccaaagttgc
    29701 ggctaacttc ttcgatcagg tggaaaagga atggcatctt gaagcaataa gccgcgattt
    29761 gctcatcagc gcctgtcagc ttcatgaaat cggcctgagc gttgacttta aacaagcgcc
    29821 gcaacacgct gcttatctgg tgcgtaatct ggatcttccc ggttttaccc ccgcacagaa
    29881 aaaattgctg gcgacgctac tgctcaacca gactaatccg gtcgatctct catcgctgca
    29941 tcagcaaaat gccgtaccgc cgcgcgtcgc agaacaactc tgccgtttac tccgcctggc
    30001 catcattttt gccagccgtc gccgtgacga tctcgtgcca gagatgacat tacaggctaa
    30061 ccatgaactg ttgaccttga cgcttccgca aggttggcta acccaacatc cgctgggtaa
    30121 agagattatt gctcaggaaa gccagtggca gagctatgtc cactggccgc tggaagtgca
    30181 ttaatgagta agtgccggat gcgatgctga cgcatctttt ccggccttga ttatttccct
    30241 cgttttgccg ccatcatcgc tttcagattc gccagatggc tttgcccttt ctgcatccgt
    30301 tcttcggcgc tgaccacttt gcgctcctgt tcccaaatca gatcatcctg cggcagctcc
    30361 agcaaaaagc ggctcggctc cgggcgcacc agctcgccgt actgacggcg ttctttacac
    30421 agcgtaaagg tcaattcctt ctgggcgcgg gtaatgccga cataggccag ccgccgctcc
    30481 tcatcgatat tatcttcatc gatgctgctc tggtgcggca aaaacccttc ttccataccg
    30541 accatgtaga cataaggaaa ctccagccct tttgacgcgt gcagagtcat cagttgcacc
    30601 tgatccagct cttcttcact ctcaccacgc tccatcatgt cgcgcaaagt aaagcgcgtc
    30661 accacctggg tgagcgtcat cggctcatcc agttcgctgc cttccagcat ctccgtcatc
    30721 cagctaaaca gttggttgac gttcttcatg cgcatttcgg cggctttcgg gctgggcgat
    30781 gtttcgtaca gccaggattc ataatccatg ccgtggatca gatcgcgcac cgcggcaatc
    30841 ggctcccgct ccgccagacg ctggatttct gccaaccagt gagtaaagcg ggtcaatgct
    30901 tcataaccac gtccgctaag cgtctgactc aggcccatat caaagctggc ggtaaacatg
    30961 cttttattgc gcgtcatcgc ccactcaccc agctttttca gcgtcgccgg gccaatctct
    31021 ctcttcggcg tgttaacgat acgcagaaat gcgctgtcat cgtccgggtt agtcagcacg
    31081 cgcagataag ccagcaagtc cttgatttca ggacgagaga aaaacgacgt accaccagaa
    31141 attttgtacg ggatgcggtt ttgcatcagg aatttttcaa acactcgcga ctgatggtta
    31201 ccgcgataaa gaatagcgta atctttgtac tgcgttttat tgacgaagtg atgcgcaatc
    31261 agttcaccag tgacgcgctc ggcttcatgt tcttcgttat tcgcgcttaa aactttaagc
    31321 tctgtgccgt aacccagttc ggagaacaga cgcttctcaa agacgtgcgg gttattggcg
    31381 atcaggatgt tcgccgcttt caaaatacgt ccggaagagc ggtagttctg ctcaagctta
    31441 atcaccttca gcgccggaaa atcctgactc agcagcacca ggttttgtgg acgtgcgccg
    31501 cgccaggagt agatcgactg gtcgtcatcg cccaccacgg taaagcgcgc gcggctgccc
    31561 accagcagtt tcaccagttc atactggctg gtgttggtgt cctgatactc atccaccagc
    31621 agatagcgaa ttttgttctg ccagcgctcg cggacttctt cattgcgttg cagcagcaac
    31681 gtcggcaata aaatcagatc atcgaagtcg agaacattgc acgctttcag gtgtgcatca
    31741 tacagcccat aacaatgggc aaaaatacgg tcccgctcgc caatcgcact ggctgccgcc
    31801 tgggacggtg ttttgagatc gttcttccag ttagagatgg tcgaaatcaa ctgttgcagg
    31861 agaactttgt catcttcaat cagcccttcg gtcaactctt tgagcaacgc cagctgatcg
    31921 gtatcgtcaa acagcgaaaa gttcgccttc atccccagcg ccgcatactc acgcttgatg
    31981 atatccagcc ccagcgtgtg gaaggttgag atcatcagcc cacgcgcctc tttgcgaccc
    32041 agcgtctgtc cgacacgctc tttcatctcg cgcgccgcct tattggtaaa ggtcaccgcc
    32101 gcgatatgtc gcgcctgata accgcaaccg cggatcagat gggcgatttt attggtgatc
    32161 acacgagttt taccggaacc cgcgcccgcc agcaccaggc aggggccggt aacgaattcg
    32221 acagcttgtt gttggccggg gtttagacgc ataggtgtat tgctcaatct tcgaacgggg
    32281 ggaggattgt agcagaatcg catttgaatg tgaccgcgtg taagccgcta accgattggc
    32341 tgcggatgtt taaatcagag agggaagacc gaaaccggag ttcatcgtga atccattaga
    32401 cttagaaaat atcgggtcgt aacgttgtgt tattactttt tcagagttta ctaaggaga
//
LOCUS       DADMKF010000032        32458 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.033, whole genome shotgun sequence.
ACCESSION   DADMKF010000032 DADMKF010000000
VERSION     DADMKF010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32458
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.033"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            217..780
                     /locus_tag="JNC24_001362"
     CDS             217..780
                     /locus_tag="JNC24_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HAY9158153.1"
                     /translation="MSKFVKTAIAAAMVMGVFTSTATIAAGNNGTARFYGTIEDSVCS
                     IVPDDHKLEVDMGDIGAEKLKNNGTTTPKSFQIRLQDCVFDTQETMTTTFTGTVSSAN
                     SGNYYTIFNTDTCAAFNNVSLAIGDSLGTSYKSGMGIDQKIVKDTSTNKGKAKQTLNF
                     KAWLVGAADAPDLGNFEANTTFQITYL"
     gene            862..3504
                     /gene="yfcU"
                     /locus_tag="JNC24_001363"
     CDS             862..3504
                     /gene="yfcU"
                     /locus_tag="JNC24_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher protein YfcU"
                     /protein_id="HAY9158154.1"
                     /translation="MPDHSLFRLRILPWCIALAMSGSYSSVWAEDDIQFDSRFLELKG
                     DTKIDLKRFSSQGYVEPGKYNLQVQLNKQPLAEEYDIYWYAGEDDASKSYACLTPELV
                     AQFGLKEDVAKNLQWSHDAKCLKSGQLEGMEIKADLSQSALVISLPQAYLEYTYPDWD
                     PPSRWDDGISGIVADYSINAQTRHEENGGDDSNEISGNGTVGVNLGPWRMRADWQTNY
                     QHTRSNDDDEFSGDETQKKWEWSRYYAWRALPSLKAKLALGEDYLRSDIFDGFNYVGG
                     SVSTDDQMLPPNLRGYAPDISGVAHTTAKVTVSQMGRVIYETQVPAGPFRIQDLGDSV
                     SGTLHIRIEEQNGQVQEYDISTASMPYLTRPGQVRYKIMMGRPQEWGHHVEGEFFSGA
                     EASWGIANGWSLYGGALGDENYQSAALGVGRDLSTFGAVAFDVTHSHTKLDKDTAYGK
                     GSLDGNSFRVSYSKDFDQLNSRVTFAGYRFSEENFMTMSEYLDASDSGMVRTGNDKEM
                     YTATYNQNFRDAGVSVYLNYTRHTYWDREEQTNYNIMLSHYFNIGSIRNVSISMTGYR
                     YEYDNQADKGMYISLSMPWGDNSTVSYNGNYGSGTDSSQVGYFSRVDDATHYQLNVGT
                     SDKHTSVDGYYSHDGSLAQVDLSANYHEGQYTSAGLSLQGGATLTAHGGALHRTQNMG
                     GTRLLIDADGVADVPVEGNGAAVYTNMFGKAVVSDVNNYYRNQAYIDLNKLPENAEAT
                     QSVVQATLTEGAIGYRKFAVISGQKAMAVLRLQDGSHPPFGAEVKNDNEQTVGLVDDD
                     GNVYLAGVKPGEHMSVFWSGVAHCDINLPDPLPADLFNGLLLPCQHKGNVAPVVPDDI
                     KPVIQEQTQQVTPTNPPVSVSANQ"
     gene            3524..4275
                     /locus_tag="JNC24_001364"
                     /pseudo
     CDS             3524..4275
                     /locus_tag="JNC24_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416839.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
     gene            4250..4798
                     /locus_tag="JNC24_001365"
     CDS             4250..4798
                     /locus_tag="JNC24_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY9158155.1"
                     /translation="MKSRAAKAEAGRASMKGSCSVWAVTGLLICCVGSGFAVAASKSV
                     SMNLRVLVDAPPPCTVNGAAVEFGNVFINKINGVDYKRPIDYSLVCNNLAMDDLRLQM
                     QATTVVINGETVISTGIPGFGIRVQKSSDHTILDLTSGSWLPFNFSSGVPVLEAVPVK
                     QSGTTLAAAEFNASATIVVDYQ"
     gene            4795..5265
                     /locus_tag="JNC24_001366"
     CDS             4795..5265
                     /locus_tag="JNC24_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY9158156.1"
                     /translation="MKRISLILLWGFCSMALSNVSFHGYLVQPPNCTISNAQTIEITF
                     QDVLIDDINGSNYEQTVPYSITCDTAVRDPLMEMTLSWSGTLSDFDNAAVSSNITGLG
                     IQLKQAGQSFTINTPLMVNETDLPVLTAVPVKKSGVVLPEADFEAWATLQVDYQ"
     gene            5262..5786
                     /locus_tag="JNC24_001367"
     CDS             5262..5786
                     /locus_tag="JNC24_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY9158157.1"
                     /translation="MKKKRTLFFISSLMLLGSGTTIAGDNLHFTGNLISKSCTPVING
                     SQLAEVHFPAIAASDLMNLGQSERVPLVFQLKDCHSSTLFNVKVTLTGTEDSALPGFL
                     AFDSSSSASGAGIGIETAAGTSVPINNTTGVTLPLNQGNNSLNFNTWLQAKSGRDVTS
                     GDFSATVTATFEYF"
     gene            5773..6645
                     /locus_tag="JNC24_001368"
     CDS             5773..6645
                     /locus_tag="JNC24_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2544 domain-containing protein"
                     /protein_id="HAY9158158.1"
                     /translation="MSISEMKKWTIIFASLMLLVLSVVGASKSYAGDKLMSASFDSVR
                     VYYAMDKVTGAVASSVFNVTVITPKEVTYGKYDSFAYKGDTLRVISWSGSGSAPTLVL
                     TDFDTINNSNCPGIDTKIFRCAYMTFKITVASDDYGCPWIASFYSYTDLPGFGSYTAP
                     TVHNTICPTIPVTSYDISWSENYVSHNKALRIQSTGSTVTTTLSTYLMEGGRLCDGSN
                     FSDNDGRGAYCRAVSELLTFTSYGCDKSTVTVTPTRHPVTDKVLHDIVVNVNTSSGQP
                     IDSTCRFQYVLNEL"
     gene            complement(6767..7318)
                     /gene="smrB"
                     /locus_tag="JNC24_001369"
     CDS             complement(6767..7318)
                     /gene="smrB"
                     /locus_tag="JNC24_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HAY9158159.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIPEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            7484..8416
                     /gene="prmB"
                     /locus_tag="JNC24_001370"
     CDS             7484..8416
                     /gene="prmB"
                     /locus_tag="JNC24_001370"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HAY9158160.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLLAAREYFAIYKD"
     gene            8451..9536
                     /gene="aroC"
                     /locus_tag="JNC24_001371"
     CDS             8451..9536
                     /gene="aroC"
                     /locus_tag="JNC24_001371"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HAY9158161.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     LGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            9540..10364
                     /gene="mepA"
                     /locus_tag="JNC24_001372"
     CDS             9540..10364
                     /gene="mepA"
                     /locus_tag="JNC24_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HAY9158162.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPSGDGCGAELQSWFAPLKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            10364..11173
                     /locus_tag="JNC24_001373"
     CDS             10364..11173
                     /locus_tag="JNC24_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HAY9158163.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVTLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            11173..11721
                     /locus_tag="JNC24_001374"
     CDS             11173..11721
                     /locus_tag="JNC24_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HAY9158164.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     ALDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLTNGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            11755..12033
                     /locus_tag="JNC24_001375"
     CDS             11755..12033
                     /locus_tag="JNC24_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HAY9158165.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQQ"
     gene            complement(12154..14160)
                     /gene="mnmC"
                     /locus_tag="JNC24_001376"
     CDS             complement(12154..14160)
                     /gene="mnmC"
                     /locus_tag="JNC24_001376"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HAY9158166.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVVAESGFGTGLNFLTLWQAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLNEGRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQEAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSTPWFNRTGSSKREVAIIGGGIAS
                     ALLSLALLRRGWQVALYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLTALNPNRLWVRKLLKGKAVKAG"
     gene            14319..15539
                     /gene="fabB"
                     /locus_tag="JNC24_001377"
     CDS             14319..15539
                     /gene="fabB"
                     /locus_tag="JNC24_001377"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HAY9158167.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNVKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAHIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMQMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            15814..16992
                     /locus_tag="JNC24_001378"
     CDS             15814..16992
                     /locus_tag="JNC24_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY9158168.1"
                     /translation="MTAVSQTETRSSANFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     ELGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVIGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPLLAWACNGTVRKVPALAGERPSLWSVVGFI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTEFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(16989..17984)
                     /locus_tag="JNC24_001379"
     CDS             complement(16989..17984)
                     /locus_tag="JNC24_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HAY9158169.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVNDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            18083..19219
                     /gene="pdxB"
                     /locus_tag="JNC24_001380"
     CDS             18083..19219
                     /gene="pdxB"
                     /locus_tag="JNC24_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HAY9158170.1"
                     /translation="MKILVDENMPYARDLFSRLGEVTAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQHADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            19285..20298
                     /locus_tag="JNC24_001381"
     CDS             19285..20298
                     /locus_tag="JNC24_001381"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HAY9158171.1"
                     /translation="MSEGWNIAVLGATGAVGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            20298..21110
                     /gene="truA"
                     /locus_tag="JNC24_001382"
     CDS             20298..21110
                     /gene="truA"
                     /locus_tag="JNC24_001382"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAY9158172.1"
                     /translation="MSDQQQLPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPERWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            21193..21852
                     /locus_tag="JNC24_001383"
     CDS             21193..21852
                     /locus_tag="JNC24_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HAY9158173.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VTPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGDKLFS
                     NPDSKIFRRSYLDKTHQFYERHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLIFTYAGYFFGTLPFIQSNLKLMIVGIIFVSILPGVFEFIRHKRAAARAA
                     K"
     gene            22008..22922
                     /gene="accD"
                     /locus_tag="JNC24_001384"
     CDS             22008..22922
                     /gene="accD"
                     /locus_tag="JNC24_001384"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HAY9158174.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDVLKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQALEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPGFQRSEFLIEKGAIDMIVHRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            22992..24260
                     /gene="folC"
                     /locus_tag="JNC24_001385"
     CDS             22992..24260
                     /gene="folC"
                     /locus_tag="JNC24_001385"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HAY9158175.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSAKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            24250..24912
                     /gene="dedD"
                     /locus_tag="JNC24_001386"
     CDS             24250..24912
                     /gene="dedD"
                     /locus_tag="JNC24_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HAY9158176.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            25171..25659
                     /gene="cvpA"
                     /locus_tag="JNC24_001387"
     CDS             25171..25659
                     /gene="cvpA"
                     /locus_tag="JNC24_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HAY9158177.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            25696..27213
                     /gene="purF"
                     /locus_tag="JNC24_001388"
     CDS             25696..27213
                     /gene="purF"
                     /locus_tag="JNC24_001388"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HAY9158178.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            27308..27877
                     /locus_tag="JNC24_001389"
     CDS             27308..27877
                     /locus_tag="JNC24_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HAY9158179.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTHDARDISASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            28143..28925
                     /gene="argT"
                     /locus_tag="JNC24_001390"
     CDS             28143..28925
                     /gene="argT"
                     /locus_tag="JNC24_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HAY9158180.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYAPFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKAFAFAGSSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            29146..29928
                     /gene="hisJ"
                     /locus_tag="JNC24_001391"
     CDS             29146..29928
                     /gene="hisJ"
                     /locus_tag="JNC24_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HAY9158181.1"
                     /translation="MKKLVLSLSLVLAFSSATAAFAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIDLAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            30018..30704
                     /locus_tag="JNC24_001392"
     CDS             30018..30704
                     /locus_tag="JNC24_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311219.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAY9158182.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNTVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            30701..31417
                     /locus_tag="JNC24_001393"
     CDS             30701..31417
                     /locus_tag="JNC24_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY9158183.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            31425..32198
                     /gene="hisP"
                     /locus_tag="JNC24_001394"
     CDS             31425..32198
                     /gene="hisP"
                     /locus_tag="JNC24_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HAY9158184.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
BASE COUNT         7618 a         8026 c         8761 g         8053 t
ORIGIN      
        1 taattaaatc tacatttact ctttagtaaa attaaattgc atcaggggtg tggaaacatc
       61 actatgcggc cccacaccaa cgaatacaat tttctacgac ctgttctttt tggatcgcat
      121 gccgtattca atggctgggt ataaacattg gttattgact gaatcaatgt attgcaatgt
      181 ttatattttt aattatttat agtttaagga caaataatga gtaagtttgt taaaacagct
      241 attgctgcgg caatggtgat gggcgtgttc acctctacgg caacaatcgc tgcgggtaac
      301 aacggtactg cacgttttta cggcaccatt gaagactcag tgtgttctat cgttccggac
      361 gatcacaaac tggaagttga tatgggtgat atcggtgccg aaaaactgaa aaataacggc
      421 accaccacgc cgaaaagttt ccagattcgt ctgcaagatt gcgtatttga tactcaggaa
      481 acaatgacca ctacctttac tggtaccgtt tcttctgcaa atagcggcaa ttattacacc
      541 attttcaata ccgatacctg tgcggcattt aacaatgtta gcctggcgat cggtgactct
      601 ctgggtacct cttacaaaag cggcatgggt attgaccaga aaatagtgaa agacacttct
      661 accaataaag gcaaagcgaa gcagacactg aactttaaag cctggctggt cggcgcagct
      721 gatgcgccag atctgggtaa tttcgaagca aataccacct tccagattac ttacctgtaa
      781 ggcatacccc catagcgaca ctgctatggg ggatttaaat ggattttgac cgaacaagaa
      841 taaaaacgga tagaaacgtg tatgcctgac cattctcttt ttcgattacg gattcttccg
      901 tggtgcattg cgctggcaat gtcagggagt tatagcagtg tctgggctga agacgacatt
      961 cagtttgatt cccgttttct ggaattaaaa ggcgacacaa aaattgatct gaagcgtttt
     1021 tccagccagg gatatgttga gcccggaaaa tacaatttac aggttcaact aaataaacag
     1081 ccattggcgg aagagtacga tatttactgg tatgctggtg aagatgacgc gagcaaaagc
     1141 tatgcttgtc tgacaccgga actggtagcg cagtttggtt taaaagaaga cgtggcgaaa
     1201 aatctgcaat ggagccacga tgctaaatgc ctgaaatccg gtcaactgga aggcatggaa
     1261 attaaggctg atttaagcca gtccgcatta gtcatttcac tgccacaggc ttacctcgaa
     1321 tatacttatc ccgactggga tccgccttca cgttgggatg acggcatctc cgggatcgtc
     1381 gcggactaca gcatcaacgc acaaacccgg cacgaagaaa atggcggtga tgatagtaac
     1441 gagatcagcg gcaacgggac ggtcggggtt aacctggggc cgtggcgtat gcgtgctgac
     1501 tggcagacta actatcaaca tactcgcagt aatgatgacg atgaattcag cggcgatgaa
     1561 actcaaaaaa aatgggagtg gagtcgctac tatgcctggc gggcgttacc atcattaaaa
     1621 gccaaactgg cgctgggcga ggattacctc agatccgata tttttgatgg ttttaactat
     1681 gttggtggca gtgtcagtac tgacgatcaa atgttgcctc ccaatctgcg cggctacgcg
     1741 ccagacattt ccggcgtggc acacaccaca gcaaaagtga ccgtcagcca gatggggcgt
     1801 gtgatttacg aaacgcaggt tccggctgga ccgtttcgta ttcaggatct tggtgattct
     1861 gtctccggta cgttgcatat tcgcattgaa gaacagaacg gccaggtgca ggaatatgac
     1921 atcagcaccg cctcgatgcc atacctcact cgtccaggtc aggttcgcta taagatcatg
     1981 atgggccgtc cgcaagagtg gggacaccat gtcgagggtg aatttttttc tggtgctgaa
     2041 gcttcctggg ggatcgctaa cggctggtcg ttatatggcg gcgcactggg agatgaaaac
     2101 tatcagtctg cggcgcttgg cgtcggtcgc gatttgtcta cattcggcgc ggtcgcgttt
     2161 gatgttactc actcgcacac caaactggat aaagacaccg cttatggcaa aggttcgctg
     2221 gacggtaact ccttccgtgt gagttattcc aaagactttg accagctcaa cagccgcgtt
     2281 acttttgctg gatatcgctt ctcggaagag aactttatga ccatgagcga gtatctggat
     2341 gccagtgaca gcggaatggt acgcacgggc aacgacaaag agatgtacac tgccacttat
     2401 aaccagaact tccgcgatgc gggtgtttcg gtttatctca actatacccg ccatacctac
     2461 tgggatcgcg aggagcagac aaactacaac atcatgctct cgcactattt caatataggc
     2521 agtattcgta atgtcagcat ctcgatgact ggctaccgtt acgagtatga caaccaggcc
     2581 gacaaaggca tgtacatttc gctcagtatg ccgtggggtg acaacagtac cgttagctat
     2641 aacggtaact atggcagtgg gacggacagc agtcaggtcg gttatttcag ccgtgtcgat
     2701 gacgcgactc actatcagtt gaacgtcggc accagtgaca aacacaccag cgttgacggc
     2761 tattacagcc atgatggttc gctggcgcag gttgacctca gtgcgaacta ccatgaaggg
     2821 caatacacct ctgcgggctt gtcgttacag ggcggcgcaa cgcttactgc ccacggtggc
     2881 gcacttcacc gtacccagaa tatgggcggg acacgcttgt tgattgatgc cgatggcgtt
     2941 gccgatgttc cggtagaagg taacggtgct gctgtttata ccaatatgtt tggtaaagcc
     3001 gtcgtttctg acgtcaataa ctattaccgc aatcaggcgt atatcgacct caacaaactg
     3061 ccggaaaacg ccgaagcaac ccagtcggtg gtacaagcca cgctaactga aggggcgatt
     3121 ggctaccgca aatttgccgt catcagtggt caaaaagcga tggcggtgct gcgtttacaa
     3181 gacggcagcc atcctccgtt tggcgcagaa gtaaaaaatg ataacgagca gacagtgggc
     3241 cttgtcgatg atgacggcaa tgtttatctg gctggggtga aacctggcga acacatgagt
     3301 gtgttctgga gtggtgttgc gcattgcgat atcaacctgc cggacccgct gcctgccgat
     3361 ctgtttaacg gcttgttact gccatgccag cataaaggca acgtcgcgcc ggttgttcct
     3421 gatgacataa agcctgtcat tcaggagcag acgcaacagg tgacacccac caatccccct
     3481 gtttctgttt cagctaacca ataacgtgat taaggaatga tccatgtctg atttactttg
     3541 ctctgccaaa ttgggtgcta cgacgctagc gttactgctt agcgctgcca gtttgtcgcc
     3601 caggcttctg taacgccaga tcgtacccgt ttgatattta atgaaagcga caaatcaatc
     3661 agcgtgacgt tgcgcaataa cgatccgaaa ctgccttatc tggcccaaag ctggatcgaa
     3721 gatgaaaagg gtaacaagat cagttcaccg ttaactgttc tgccaccggt gcaacgcatc
     3781 gattcaatga tgaacgggca ggtaaaagtg cagggaatgc cggatatcaa caaattgcct
     3841 gccgatcgtg aaagcctgtt ctacttcaac gtgcgtgaga tcccgccaaa atcaaacaaa
     3901 gccaacacgc tgcaaatagc actacaaacg cgtattaagt tgttctggcg accgaaggcg
     3961 ctggagaacg tcagcatgaa aaacccctgg caatataagg ttactttaac ccgcaacggt
     4021 caggaattta ccgtcaataa cccgacgccg tattacgtca ttttcagtaa tgccagcaca
     4081 cagaaaaatg gcaatcctgc agccggtttc tcgccaatag tgatgtcgcc gaaaacctct
     4141 gcaccgctga atgtgaagat gggtagcgtg ccggtactga cctatgtcaa cgactatggc
     4201 gctcggatgc cgctcttttt cacttgcaac ggtaacagtt gccaggtcga tgaagagcag
     4261 agccgcaaag gctgaggccg ggagagccag tatgaaagga tcttgttctg tctgggcggt
     4321 aactgggtta ctgatatgtt gtgttggtag cggctttgca gttgcagcga gtaagtcagt
     4381 aagtatgaat ttacgggtgc ttgtcgatgc ccctcctcct tgtacggtaa atggtgcggc
     4441 agttgagttt ggtaatgtat tcattaataa gatcaatggg gttgattata aacgtcccat
     4501 tgactactcg cttgtttgta acaatctggc aatggatgac ctgcgtctac agatgcaggc
     4561 gacaacggtc gtgattaacg gtgaaacagt tatcagcacg ggtattcctg ggtttggtat
     4621 tcgggttcag aaaagtagtg atcacacaat actcgactta acctctggct catggttgcc
     4681 atttaatttt tcctcgggag ttcccgttct ggaagctgtg ccggtgaaac agagtggtac
     4741 aacgttagcg gcagctgaat ttaacgccag tgccacaatt gtggtggatt accaatgaaa
     4801 cggatctcgc ttattttgct gtggggcttt tgctcgatgg cactctcaaa cgtctctttt
     4861 cacggttatt tagtgcagcc gcctaactgc acgattagca acgctcaaac cattgaaata
     4921 acttttcagg atgtgcttat tgatgacatt aatggcagca attatgagca aaccgtgccg
     4981 tattccatta cctgcgatac ggcggttcgc gacccattaa tggaaatgac cttgagctgg
     5041 agtggcacac taagcgattt tgataatgct gcagtaagca gcaatatcac tggtttaggc
     5101 attcagctca aacaggcagg gcagagtttt acgatcaata cgccacttat ggtgaatgaa
     5161 accgatttac cggtgttaac ggctgtgcca gtaaaaaaga gtggtgtcgt tctgccagag
     5221 gcggactttg aggcctgggc cacgttacag gtagattacc aatgaagaaa aagcgtacgt
     5281 tgttttttat tagtagcctg atgttgctgg gcagtggaac cacaatagca ggagataatt
     5341 tgcactttac cggcaattta atcagtaaat cgtgtacgcc agtgattaat ggtagccagt
     5401 tggcagaagt acattttcct gccattgccg ccagcgattt aatgaacctt gggcagtcag
     5461 agcgagtgcc attggtgttt caattgaaag attgtcatag ttctacattg tttaatgtca
     5521 aagtaacgtt gacaggaaca gaagatagtg ctctacccgg ctttctggcc tttgactcct
     5581 cgtcatcagc atccggcgct gggattggta ttgaaactgc ggcaggcaca tcggtgccaa
     5641 tcaacaatac aacaggcgtc acgctcccgc tgaatcaggg aaataacagc ctaaatttta
     5701 atacctggtt acaggcaaag agcgggcgag atgtaacgtc cggagacttt agtgccacag
     5761 tgacggcaac ctttgagtat ttctgaaatg aaaaaatgga ccataatttt cgcgagccta
     5821 atgctgctcg ttctgagtgt tgtcggcgca tctaagagtt atgctggaga taaacttatg
     5881 agtgcgagtt ttgacagtgt gagagtttat tatgccatgg acaaggtaac cggtgctgta
     5941 gcgagcagtg ttttcaatgt tacggtgata acgccaaagg aggttacata tgggaaatat
     6001 gattcttttg cgtataaagg cgatacgttg cgggttattt cctggtctgg gtcgggttca
     6061 gctccaactt tggtactgac tgactttgat acaattaata actcaaattg ccctggcata
     6121 gatacgaaaa tttttcgttg tgcatatatg acgtttaaaa ttaccgttgc atctgatgat
     6181 tatggttgcc cttggatcgc atccttttat tcctatacag atttacccgg atttggaagc
     6241 tatacggccc ctacagtgca taacacaata tgcccaacga taccggtcac ttcatacgat
     6301 atctcctgga gtgaaaatta tgtcagccat aataaggcgt tacgaattca gtccacgggg
     6361 tcaacagtga ccaccacgct ttcgacctac ctgatggaag gcggcagact ctgtgatggc
     6421 agtaattttt ctgataatga tggcagagga gcatactgcc gcgcggtatc agaattgtta
     6481 acctttacct cttacggttg tgataaatca acggtaacgg ttacgccaac tcgtcatccg
     6541 gtaacggata aagtactaca cgatattgtg gtgaatgtga ataccagtag tgggcaaccg
     6601 attgattcga cgtgtcgttt tcagtatgta ttgaatgaac tgtagttcgt agtattgctc
     6661 tgatgcgtct tatcaggcct acgaagtgaa ttgcaattta ttgaattatc acatatttgt
     6721 aggccggata aggcgtttac gctgctccgg catttgctgc cagcattcag ggcaactccg
     6781 gcggcaacca ctcttccact tcaatcaaca ccaacaaagc cgcatcaccg ccgtactctt
     6841 tcggtgcctg gtgaaatgcc atcacatgcg gatgttgtgc cagccacagc ggtgtttgtt
     6901 gcttcaaaat atgcttcccg tgcccatgca tcacgcaggc acaaaacaca tgttcgcggc
     6961 gacaggcggc aatcaacgcg cccagttcct gcttggcctg cagctgcgtc agaccgtgta
     7021 aatccaaaaa caattccggt gaataatcgc cgcggcgcag tttcttcgcc tcaaaatggc
     7081 tgacatccgg gcgaacatat ttcaccggac cttcggtatt taataacggc tgaaactcat
     7141 cggagaaata atggctggca tcagcctgct ccgggatcaa gcgtttcacc ggcacttcgc
     7201 tgattttttt acgctgcggt cggtggacaa tcgtgtcctg cttaatcttg cgagtccccg
     7261 ccatcaactg gcgaaacaga gcctggtcct cctcgctaag tgttgttttc tttttcattt
     7321 atctgtctct atttctaatt ttcctacagt ttacccgact cgtagcacaa tcgatcgttt
     7381 taaacgtatt tttcctcact caccgcgcgt gaatgctgat ttatcgtcat cttcatggca
     7441 aactagccgc cgaaattatt gcgatcatgc cctggaggaa tacgtggata aaattttcgt
     7501 tgatgaagca gtaaatgagc tgcaaaccat tcaggacatg ttgcgctggt cggtaagccg
     7561 cttcagcgcg gcaaatatct ggtacggtca cggcaccgat aacccgtggg atgaagccgt
     7621 acagctggtg ttaccatcgc tctacctgcc gctggatatc ccggaagata tgcgcaccgc
     7681 gcgtctgacc tccagcgaaa aacaccgtat tgttgaacgc gtgatccgcc gcgtcaacga
     7741 gcgcattccg gtggcttacc tgaccaacaa agcctggttc tgcggtcatg aattttacgt
     7801 cgatgaacgc gtgctggtgc cgcgctcgcc gattggtgaa ctgatcaaca ataaatttgc
     7861 cggactcatt agcaagcaac cgcagcatat tttagatatg tgtaccggta gcggctgcat
     7921 cgccattgcc tgtgcttatg ccttcccgga agcggaagtc gacgcggtag atatctctcc
     7981 cgacgcgctg gcggttgccg aacagaacat cgaagaacac ggtctgatcc acaacgtcat
     8041 tccgattcgt tccgatctgt tccgtgattt gccgaaagtg cagtacgatc tgattgtcac
     8101 taacccgccg tatgtcgacg cggaagatat gtccgacctg ccaaacgaat accgtcacga
     8161 gccggaactg ggcctggcat ctggcaccga cggccttaaa ctgacgcgtc gcattcttgg
     8221 aaacgcggca gattaccttg ctgatgatgg cgtgttgatt tgtgaagtcg gcaacagcat
     8281 ggtacatctt atggaacaat atccggatgt tccgttcacc tggctggagt ttgataacgg
     8341 cggcgatggt gtgtttatgc tcaccaaaga gcagctgctg gcggcccgtg aatatttcgc
     8401 gatttataaa gattaagtaa acacgcaaac acaacaataa cggagccgtg atggctggaa
     8461 acacaattgg acaactcttt cgcgtaacca ccttcggcga atcgcatggg ctggcgctcg
     8521 gctgcatcgt cgatggtgtt ccgccaggca ttccgctgac ggaagcggac ctgcaacatg
     8581 acctcgaccg tcgtcgccct gggacatcgc gctataccac ccagcgccgc gagccggatc
     8641 aggtcaaaat tctctccggt gtttttgaag gcgttactac tggcaccagc attggcttgt
     8701 tgatcgaaaa cactgaccag cgctctcagg attacagcgc aattaaagac gttttccgcc
     8761 caggccatgc tgattacacc tacgaacaaa aatacggtct gcgcgattat cgcggcggtg
     8821 gacgttcttc cgcccgcgaa accgccatgc gcgtggcggc aggggcgatt gccaaaaaat
     8881 atctcgccga gaaatttggt attgaaatcc gcggctgctt gacccagatg ggcgacattc
     8941 cgctggaaat caaagactgg tcgctggttg aacaaaatcc gttcttctgc ccggacccgg
     9001 acaaaatcga cgcgttagat gaactgatgc gcgcgctgaa aaaagagggc gactccatcg
     9061 gcgcgaaagt caccgttgtt gccagtggtg tccccgccgg acttggcgag ccggtctttg
     9121 accgcctgga tgccgacatc gcccatgcgc tgatgagcat caacgcggtg aaaggcgtgg
     9181 aaattggcga cggttttgac gtagtggcgc tgcgcggcag ccagaaccgc gacgaaatca
     9241 ccaaagacgg tttccagagc aaccatgcgg gcggcattct cggcggtatc agcagcgggc
     9301 agcaaatcat tgctcatatg gcgctgaaac cgacctccag cattaccgtg ccggggcgca
     9361 ctattaaccg ctttggcgaa gaagttgaga tgatcaccaa aggccgtcac gatccctgtg
     9421 tcgggatccg tgcagtgccg atcgctgaag cgatgctggc gatcgtttta atggatcacc
     9481 tgttacggca gcgggcgcaa aatgccgatg tgaagactga tattccacgc tggtaaaaaa
     9541 tgaacaaaac cgcgattgcg ctgctggctc tgcttgccag tagcgctagc ctggcagcga
     9601 cgccgtggca aaaaataacc caacctgtgc cgggtagcgc acaatcgata ggcagttttt
     9661 ctaatggctg tattgtcggc gctgacacgt tgccgataca gtccgaacat tatcaggtca
     9721 tgcgtaccga tcagcgtcgc tatttcggtc acccggatct ggtgatgttt atccagcgtc
     9781 tgagtagcca ggtgagcaat ctgggcatgg gtacggtgct gattggcgat atggggatgc
     9841 ccgctggtgg gcgtttcaac ggcggtcatg ccagccacca gaccggactg gatgtcgata
     9901 tctttctgca actgccgaaa actcgctgga cctccgcgca gctcttgcgc ccgcaagcac
     9961 tggacttagt ttcccgcgac ggtaaacacg ttgtctccac gctgtggaag ccagaaattt
    10021 tcagcttgat caaactcgct gcccaggaca aagacgtcac gcgcattttt gttaatccgg
    10081 cgattaaaca acaactttgc cttgatgcgg gcaccgatcg cgactggttg cgcaaagtgc
    10141 gaccctggtt ccagcatcgc gcgcatatgc atgtacgatt acgttgccct gccgatagtc
    10201 tggagtgtga agatcaacct ttaccgccat caggcgatgg ttgcggggca gaactgcaaa
    10261 gctggtttgc acctctaaaa ccgggaacaa caaagcctga gaagaagaca ccgcctccgt
    10321 tgccgccttc ctgccaggcg ctactggatg agcacgtgat ctaatggaaa cgtttaatag
    10381 cctgtttatg gtttccccgc tgttgctggg agttctcttt tttgtcgcca tgctggcggg
    10441 atttatcgac tcgattgccg gtggcggtgg gttactcacc attccggcat tgatggctgc
    10501 gggaatgtct cccgctaacg cgctggcaac caataaactg caagcctgcg gcggctctat
    10561 ttccgccact atctacttta ttcgccgcaa agtggttagt ttaagcgatc agaaactcaa
    10621 tattgccatg acctttgtcg gctcaatgag cggcgcactg ttggtacagt acgttcaggc
    10681 tgatgtctta cggcagattt tgcccattct ggtgatttgt atcggcctct atttcttgct
    10741 gatgccaaag ctgggtgaag aagatcgcca gcgccggatg tacggtttac ccttcgcgtt
    10801 aattgccggt ggctgcgtcg gtttttatga cggattcttt ggcccggctg ccggatcgtt
    10861 ttacgcgctg gccttcgtta cgctgtgcgg attcaacctc gccaaagcca cggctcacgc
    10921 caaattactc aacgcaacgt caaacattgg cggtctgctg ctgtttattc tcggcggcaa
    10981 agtgatttgg gcgacgggct ttgtcatgct ggtggggcag ttcctcggcg cgcgcatggg
    11041 ttcacggctg gtgttgagca aaggacaaaa gctgatccgc ccgatgattg ttatcgtctc
    11101 ggcggtgatg agtgccaaat tactttacga tagccacgga caggagatcc tccactggtt
    11161 ggggatgaac taatgaacag tacacaccac tatgagcagt tgattgaaat ttttaatagc
    11221 tgctttgccg atgaatttaa tacccgtctg attaaaggcg acgacgaacc gatctatctt
    11281 cctgctgatg cggaagtacc gtataaccga attgtctttg cccacggctt ttatgccagc
    11341 gcaattcatg agatttcgca ctggtgtatt gccggaaaag cgcgccgtga gctggttgat
    11401 ttcggctact ggtattgccc ggatgggcgt gatgcccaaa cgcaaagcca gtttgaagat
    11461 gttgaagtga agccgcaggc actcgactgg ttgttctgtg tggcggcggg atatccgttt
    11521 aacgtcagtt gcgacaatct ggaaggcgac ttcgaaccgg atcgtgttgt gtttcaacgg
    11581 cgggtacatg cgcaggtgat ggattatctg acaaatggca ttcccgagcg tccggcacgc
    11641 ttcattaaag cactacaaaa ttattatcac acgccggaac ttacggcgga acagttcccg
    11701 tggccggaag cgctcaactg aagccggtgc cacgttttac aatgaggaaa gaagatgatc
    11761 gcggagtttg aatcacgcat tctggcatta atcgacggca tggttgacca tgccagtgat
    11821 gatgagttgt ttgccagtgg gtatttgcgt ggccacctga cattagccat cgcagaactg
    11881 gaaagtggtg atgaccactc cgctcaggcg gtgcatacga ccgttagcca gagtctggaa
    11941 aaagccattg gtgccggtga attgtcgccg cgtgaccagg cgctggtgac cgatatgtgg
    12001 gaaaacctgt ttcagcaggc gtcacagcag taagttgcgg cgtctggagc accatgcctg
    12061 atgcgacgct tatcgcgtct tatcaggcct acacgtcaca cgtaggtcgg ataaggcgtt
    12121 cacgccgcat ccggcaaaca ctgcgagagc agattacccc gccttaaccg ctttaccctt
    12181 caacaatttc cgcacccata accgattcgg gtttaacgcc gtcagcgtac tggcatccat
    12241 cggaatcggt tcgtcgctca tctgcgccgc cagaatctcg gcacacagcg gggcagaaca
    12301 caaaccgcga gaacctaaag ccgcaaacat aaagagatcg tcaaaaaccg gcgcgcttac
    12361 cgcctcatct ttctgctccg ccaacgacgc atattccacg agtgttgcct cataatcggg
    12421 aacattgcct accattggca gatgatcgcg ggtggcacaa cgcacaccgc agcgcgcctc
    12481 tttatcactg acatcaacct cttttgccca ctgtgcctgc gggaaacaat caatcaaccg
    12541 ctggcgattc tgctgctgat cgtcctcact gtacgccgta tcttcgctgc cgcgatgata
    12601 actggcacca atacaatgat gttgattcgc cggattttgt ggcgtgagat aaccgtcata
    12661 gcacagcacc tgcttcagct ctgccagttc cggcgttgtc ggaatatggc tgacctgccc
    12721 ggcaaccgaa tacaccggga gagtcgacgt ttggctgaaa cggctgattt gatgcccgtt
    12781 cgccagtacc actacgctgt gtgttgcttg ctgatctcct gcaaaattca acaaccaaca
    12841 gtcatcctta cgggataaat tctgtaactg atattgataa taaatctgca aaccctgctg
    12901 ttgcgccagt tccagcacat tacgggtcag ttctgctggg cacagccaac caccttgcgg
    12961 ataagtaatg ccgctgcaat ttgtcgcaac gcccgtaatt tgttcaaccg catttgcctc
    13021 aacggctaca gccagttctg cgggtaaatc cattgacaac atctgtgcga ttttatgctg
    13081 gcttttctca tcccagccta actgcgtgac gccgcaccag tcatgatcaa atttaacggg
    13141 taattggtcg taaaaccgac gagcaaaagt aaacgcatta gagaaaaagc ggtttagcgc
    13201 ctcatcgtgt ttgcttaata acggatacag cgccccctgg cgattgccgg aagcacccag
    13261 cgcgggggct tcatccgcgc aataaagcgc tacctgccag ccgcgccgta ataacgccag
    13321 cgacaacaac gcgctggcaa taccaccgcc gataatcgct acttcccgtt tgctgctgcc
    13381 cgtacgatta aaccacggcg tggagcaggg gagtggtaat gtctgttcca tcaccccaca
    13441 aagcatttcc cgtttgcgcc caaagccctt acgtttttgc atcgtgaatc cggcttcctg
    13501 caaaccgcgg cggacaaaac cggcagacgt aaatgtcgcc agcgtgccgc ctggacgcgc
    13561 caaccttgcc atggcgttaa acagattttg cgtccacata tccgggtttt tagctggcgc
    13621 aaagccgtcc agaaaccagg catctacttt ttgatttagc gaatcgtcca gttggctggt
    13681 cagttcgtta atatcgccaa accataaatc cagcgtcacg cggccttcat tgagcaataa
    13741 acgatggcaa ccgggcaagg gcattggcca ctgcgcctga agttgttctg cccacggagc
    13801 cagttccggc cagtgttgat gcgctaaggc taaatccgca cgggtgaggg gaaatttctc
    13861 aaaactaatg aaatgtaagc gttgtaattg cgcttgcgga tgcgcttcgc gaaactgatc
    13921 aaatgcctgc catagcgtca ggaagtttaa tccggtgccg aagccgctct ctgctaccac
    13981 aaacagagga tgtggatgct caggaaagcg tacctctaat tggttgcctc ccagaaaaac
    14041 ataacgcgtc tcttccagcc cgttatcgtt ggaaaaatag acatcgtcaa aatctcggga
    14101 aacaggtgta ccctcagcat taaattcgag gttggcaggt tgtatggagt agtgtttcac
    14161 gtaagttact cgtcttacag gcggtggctc gatcttagcg atgtgtgtaa ggctgcgcaa
    14221 atttctctat taaatggctg atcggacttg ttcggcgtac aagtgtacgc tattgtgcat
    14281 tcgaaactta ctctatgtgc gacttacaga ggtattgaat gaaacgtgca gtgattactg
    14341 gcctgggcat tgtttccagc atcggtaata accagcagga agtcctggca tctctgcgtg
    14401 aaggacgttc agggatcact ttctctcagg agctgaagga ttccggcatg cgtagccacg
    14461 tctggggcaa cgtaaaactg gataccactg gcctcattga ccgcaaagtt gtgcgcttta
    14521 tgagcgacgc atccatttat gcattccttt ctatggagca ggcaatcgca gatgcgggcc
    14581 tttctccgga agcttaccag aataacccgc gcgttggcct gattgcaggt tctggcggcg
    14641 gttccccgcg tttccaggta ttcggcgctg acgcaatgcg cggcccgcgc ggcctgaaag
    14701 cggttggccc gtatgtggtc accaaagcga tggcatccgg cgtttctgcc tgcctcgcca
    14761 cgccgtttaa aattcatggc gttaactact ccatcagctc cgcgtgtgcg acttccgcac
    14821 actgtatcgg taacgcagta gagcagatcc aactgggcaa acaggacatc gtgtttgctg
    14881 gcggcggcga agagctgtgc tgggaaatgg cctgcgaatt cgacgcgatg ggtgcgctgt
    14941 ctactaaata caacgacacc ccggaaaaag cctcccgtac ttacgacgct caccgtgacg
    15001 gtttcgttat cgctggcggc ggcggtatgg tagtggttga agagctggaa cacgcgctgg
    15061 cgcgtggtgc tcacatctat gctgaaatcg ttggctacgg cgcaacctct gatggtgcag
    15121 acatggttgc tccgtctggc gaaggcgcag tacgttgtat gcagatggcg atgcatggcg
    15181 ttgatacccc aatcgattac ctgaactctc acggtacttc gactccggtt ggcgacgtga
    15241 aagagctggc agctatccgt gaagtgttcg gcgataagag cccggcgatt tctgcaacca
    15301 aagccatgac cggtcactca ctgggcgctg cgggtgtgca ggaagctatc tactctctgc
    15361 tgatgctgga acacggcttt atcgccccga gcatcaacat tgaagagctg gacgagcagg
    15421 ctgcgggtct gaacatcgtg accgaaacga ccgatcgcga actgaccact gttatgtcta
    15481 acagcttcgg cttcggcggc accaacgcca cgctggtaat gcgcaagctg aaagattaat
    15541 tcgcagtagg ccggagtagg tcggataaga cgcgccagcg tcgcatccga cgttacgcgc
    15601 caatgcggcc tccggcacta acgcaaaagg gaacctgatg gttccctttt tcacatcatt
    15661 gacaatcgcc accagttcca ggcaaacttc ccgctttgtc gatttccttc tgaaaagacg
    15721 tacgcgttaa atcctgccaa cgcaccgtaa ccctgaaacc agagagatga gacggggata
    15781 ctcctcgcct tgcgctgcat tctggagtaa tgcatgactg ctgtaagcca aaccgaaaca
    15841 cgatcttctg ccaatttttc gctcttccgc atcgcttttg cggtttttct cacctacatg
    15901 accgtagggc tgccgttgcc ggttatcccg ctgtttgttc atcatgaact gggctatggc
    15961 aataccatgg tcggcattgc cgtcgggatt cagtttctgg ctacggtgct gacgcgtggc
    16021 tatgccgggc gactggccga tcaatatggc gcaaaacgtt cggcgcttca ggggatgtta
    16081 gcttgtggtc tggctggcgg cgcgttgctg ctggcggcga ttttgcctgt ctccgcaccg
    16141 ttcaaatttg ccctgttggt catcgggcgt ttgattcttg ggtttggtga aagccagtta
    16201 ctgacaggcg ctctgacctg ggggttaggc atcgtagggc caaaacactc tggcaaagtg
    16261 atgtcatgga atggaatggc gatttacggt gccctcgctg ttggtgctcc gcttggcctg
    16321 ttgattcata gccattacgg ttttgccgca ctggcgatca ccacaatggt attaccctta
    16381 ctggcgtggg cctgtaacgg cacagtgcgc aaagtaccgg ccctggcggg agaacgtcca
    16441 tcgctgtgga gcgttgtcgg gtttatctgg aaaccagggt tagggctggc actacaaggc
    16501 gttggttttg cggttatcgg gactttcgtt tcgctctact ttgccagcaa aggatgggcg
    16561 atggcgggct ttactcttac cgagtttggc ggcgcatttg tcgtgatgcg cgtcatgttt
    16621 ggctggatgc cggaccgttt tggcggcgtg aaagtggcga ttgtctctct gcttgtagaa
    16681 acggtgggct tgttgctgct ctggcaagcc ccaggtgcgt gggtcgcatt agcgggcgcg
    16741 gcgttaaccg gagccggatg ttcgcttatc tttcctgcgc tgggcgtgga ggtggttaaa
    16801 cgcgtcccct cacaagttcg cggcaccgca ctgggcggtt acgccgcgtt tcaggatatc
    16861 gccctcggcg tctccgggcc gctggcggga atgctggcga ccacgtttgg ttactcttcg
    16921 gtatttcttg ccggggcgat ctctgcggtg ctgggaatta ttgtcacgat actgtcattt
    16981 cgtcggggct aacgaaccag ccagaccagc atcaggataa tcgcgactaa cagtatccac
    17041 agcgcgggac gcgttgccat attttgcaac gtgtccatca tcggtggaaa aggattaagc
    17101 agcggttgca tatcacgcgg atcaatccct ttaacccgcc gttgataaag ctgattaaga
    17161 atatcctcgg cctgggcaga agaaagcgat gattgcgcag aaagaccata tttttgctgg
    17221 atatacgccg ataacgctgc cagttcactg gcatctaaag gttgttttag cgccatctgt
    17281 agtgattcca gcgtcggcgt attttgctgg cttaacgtct ggcgcgcctg caaccaggtc
    17341 accagatggt taaacagttt cgctggaatt aactcgccat cgttcacccc ggaaagttcc
    17401 agcatcgatt gccagatctg tttgctgggt tcccccgttg ccgccgcaag tttggtcacc
    17461 agctgtttta gcgcattgtg ctccgccggt aataaaggac ggtcggtcgc ctcgcgctgc
    17521 tgtggttgcg gaataaccat cttcccttcc tgcaacaggg tgagaatggt ttttaattgc
    17581 tccggtgaga gctgattcag cggcgtctga ccaaagttat gacggatata atccgtgacc
    17641 gcctgacgat tattccccag acgtaaatac tcccctaatt gcgctaaaag ctggcgggca
    17701 gaatggcttt tttgcgcggc cagcagacgt tgcgccagat tatgctcagc ggcagggaag
    17761 tgacgcgaaa gcagcggtga atctcccgac agaccaatat cgtgcctgat acccgcccac
    17821 agttctgctc tttgttgttg cgtcagtgag gtcactttcg tcattaagct ttccagcgaa
    17881 gtacgttgct gactggataa aggctgattg cccgcgccag acggcaggtt atctccctga
    17941 cctggtggtt gcccaggagg agggccggaa ataggttgta tcattacgta tccttatacc
    18001 tgaaatcttc gcaagtatgc ctggccgcga gattatggca cacttgtccg gttaactctc
    18061 gtctcataca ggtaacacaa acgtgaaaat ccttgttgat gaaaatatgc cttatgcccg
    18121 cgacttattt agccgtttgg gtgaggtgac cgcggttccc gggcgtccaa tccccgtcgc
    18181 tcaactggca gacgcggatg cgctgatggt gcgttcggtc acgaaagtga atgaatcttt
    18241 gctggcagga aaacccatta aatttgttgg cactgccact gcggggaccg accatgtcga
    18301 tgaagcatgg ttgaagcagg cgggaattgg tttttccgct gcacctggct gtaatgcgat
    18361 tgcggtggtg gaatatgttt tctcctcact actgatgctt gccgaacgcg atggattttc
    18421 actgcacgac cgtacggtgg ggatcgtggg cgttggtaac gttggacgtc gattgcaggc
    18481 gcgactggaa gcgttaggga ttaaaacatt gctttgcgat ccgcctcgcg ctgaccgtgg
    18541 ggatgagggt gatttccgct cgctggatga attagttcag catgcggata ttctgacttt
    18601 ccatacgcca ctcttcaaag atgggccgta caaaacgctg catctggcgg atgaaaaact
    18661 gatccgtagc ctgaagccgg gagcgattct gattaacgcc tgccgtggcg cagtcgtcga
    18721 taatactgcg ttgctgacct gcctgaatga aggccagaag ttaagcgtag tgctggatgt
    18781 ctgggaaggc gaaccggaac ttaacgtgga gttgctgaca aaagtggata tcggcacgcc
    18841 gcatatcgca ggttataccc tggaaggtaa agcacgcggt actacgcaag tgtttgaagc
    18901 ctatagcaag tttattgggc atgaacagca cgttgcgctg gatacattac tgcccgcgcc
    18961 agagtttggt cgcattacgc tgcatggccc gctcgatcaa ccgacgctga aaaggctggt
    19021 gcatttggtg tatgatgtgc gccgcgatga cgcaccgctg cgtaaagtcg ccgggatacc
    19081 gggtgagttc gataaactgc gcaaaaacta tcttgagcgc cgtgaatggt catctctgta
    19141 tgtaatttgt gatgacgcca gtgcggcatc attgctgtgt aaactgggtt ttaacgccgt
    19201 tcatcatccg gcacgttaat ctcttcttca tgctctctgc tgtaacattg gcagggagct
    19261 ttgctatttc tggagtaaac caccatgtct gaaggctgga acattgccgt cctgggcgca
    19321 actggcgctg tgggcgaagc cctgcttgaa acgctggctg aacgtcagtt cccggttggg
    19381 gaaatttatg cactggcacg taacgaaagc gcaggcgaac aactgcgctt tggtggtaaa
    19441 acaatcactg tgcaggatgc cgctgaattc gactggacgc aggcgcagct ggcatttttt
    19501 gtcgcaggca aagaagctac cgctgcctgg gttgaagaag cgaccaactc aggttgcctg
    19561 gtgatcgaca gcagcggact gtttgctctc gaacccgacg taccgctggt ggtgccggaa
    19621 gtaaacccgt ttgtactgac cgattaccgc aaccggaatg tcatcgccgt accagacagt
    19681 ctgaccagcc agctgctggc ggcactgaaa ccgttaatcg atcagggcgg tttgtcgcgt
    19741 atcagtgtta ccagcctgat ttcagcctcc gcccagggca aaaaagcggt cgatgcgtta
    19801 gcggggcaga gtgcgaaatt gctcaacggc attccgattg acgaagaaga tttcttcggt
    19861 cgtcaactgg cgttcaatat gctgccgttg ttgccggata gcgaaggtag cgtgcgtgaa
    19921 gaacgtcgta tcgttgacga agtacgcaaa atcctgcagg acgaagggct gatgatttcg
    19981 gcaagcgtcg tccaggcacc ggtattctac ggtcatgccc agatggtcaa ctttgaagca
    20041 ctgcgtccgc tggcggcaga agaagcgcgt gatgcgtttg ctcagggcga agatattgtg
    20101 ctctctgaag agaacgaatt cccgactcag gtaggggatg cttcgggtac gccgcatctt
    20161 tctgttggct gcgtgcgtaa tgactacggt atgccggagc aagtccagtt ctggtcggtg
    20221 gccgataacg ttcgctttgg cggcgcgctg atggcagtaa aaatcgccga gaaactggtg
    20281 caggagtatc tgtactaatg tccgaccagc aacaactgcc agtttataaa attgcgctgg
    20341 gcattgagta cgacggcagt aagtattacg gctggcaacg gcagaatgaa gtccgcagtg
    20401 tgcaggagaa gctggaaaag gcgctctccc aggtggcgaa cgaacccatc accgtcttct
    20461 gcgccgggcg tactgacgca ggggtacacg gtaccgggca ggtggtgcat ttcgaaacaa
    20521 ccgcgcagcg caaagacgcg gcgtggacct tgggcgtaaa tgcgaattta cctggtgaca
    20581 tcgccgtgcg ttgggttaaa gctgtacctg atgattttca tgcccgattt agcgccacgg
    20641 ctcgccgtta tcgctacatc atctacaatc atcggctgcg cccggcggta ttgagtaaag
    20701 gggtaaccca tttttacgaa ccgctggacg ctgaacggat gcatcgggct gcgcaatgct
    20761 tgctgggcga gaatgatttc acctcgttcc gtgcggtgca gtgccagtcc cgaaccccgt
    20821 ggcgcaacgt tatgcatatt aatgtcacgc gtcacggtcc ttatgtggtg gtagatatca
    20881 aagcgaatgc ctttgtacat catatggtca ggaatattgt cggcagcctg atggaagtcg
    20941 gtgcccacaa ccagccggag agatggatag cagagttgct ggcggcaaag gacagaacgc
    21001 tggcggcagc aacggcaaaa gcggaagggc tgtatctggt cgcggtggat taccctgacc
    21061 ggtatgatct tccaaaaccg ccaatgggcc cgctatttct ggcggactaa cgagaataat
    21121 gctcggaaca tttcaggctg tagccaacgt agttacagcc tgaaagatga cgagtacaag
    21181 gcataggcaa atatggacct gatttatttc ctcatcgatt ttatcctgca cattgacgtg
    21241 catctggcag agctggtcgc ggagtacggc gtctgggttt atgccattct gtttttgatt
    21301 ctgttctgcg aaacggggct ggtggtaacg ccattcctgc ctggtgattc gctgctgttt
    21361 gttgctggtg cattggcgtc gctggaaact aacgatctca atgtccatat gatggtggtg
    21421 cttatgttga ttgccgctat tgtcggtgat gcagtgaact ataccattgg acggttattt
    21481 ggtgacaaac tgttcagtaa cccggattcg aaaatctttc gtcgcagtta tcttgataag
    21541 actcatcagt tttacgagag acatggcggc aaaacgatta ttctcgcccg ttttgtaccg
    21601 atcgtcagaa cgttcgcgcc gtttgttgcg ggaatgggcc acatgtcgta ccgtcatttc
    21661 gccgcttata acgtgatcgg cgcactgttg tgggtactga tttttaccta cgcgggttat
    21721 ttcttcggca cgctcccctt cattcagagc aaccttaagc tgatgattgt tggcattatt
    21781 tttgtttcaa tattgcctgg ggttttcgaa tttattcgtc acaaacgcgc tgcggcacgc
    21841 gccgcaaaat aaaaagtaac tccgcggttc gaccactttt ttatccaaag tttcgggctg
    21901 ttatgtttta atgtgcaaca ttcatggtct gttgggggca aaaatggcat tatgcgtccc
    21961 caaagataaa actggcatcg aaccaggttc agacagaaag gtccctaatg agctggattg
    22021 aacgaattaa aagcaacatt actcccaccc gcaaggcgag cattcctgaa ggggtgtgga
    22081 ctaagtgtga tagctgcggt caggttttat accgcgctga gctggaacgt aatcttgagg
    22141 tctgtccgaa gtgtgaccat cacatgcgta tgacagcgcg taatcgcctg catagcctgt
    22201 tagatgaagg aagccttgtg gagctgggta gcgagcttga gccgaaagat gtgctgaagt
    22261 ttcgtgactc caagaagtat aaagaccgtc tggcttctgc gcagaaagaa accggcgaaa
    22321 aagatgcgct ggtggtgatg aaaggcactc tgtatggaat gccggttgtc gctgcggcat
    22381 tcgagttcgc ctttatgggc ggttcaatgg ggtctgttgt gggtgcacgt ttcgtgcgtg
    22441 ccgttgagca ggcgctggaa gataactgcc cgctgatctg cttctccgcc tctggtggcg
    22501 cacgtatgca ggaagcactg atgtcgctga tgcagatggc gaaaacctct gcggcactgg
    22561 caaaaatgca ggagcgcggc ttgccgtaca tctccgtgct gaccgacccg acgatgggcg
    22621 gtgtttctgc aagtttcgcc atgctgggcg atctcaacat cgctgaaccg aaagcgttaa
    22681 ttggctttgc cggtccgcgt gttatcgaac agaccgttcg cgaaaaactg ccgccaggat
    22741 tccagcgcag tgaattcctg atcgagaaag gcgccatcga catgatcgtc catcgtccgg
    22801 aaatgcgcct gaaactggcg agcattctgg cgaagttgat gaatctgcca gcgccgaatc
    22861 ctgaagcgcc gcgtgaaggc gtagtggtac ccccggtacc ggatcaggaa cctgaggcct
    22921 gataactgat aagggcaggg ccactggctc tgcccttttg ctattctcac cgtaacgaat
    22981 cagcggatac catgattatc aaacgcactc ctcaagccgc gtcgcctctg gcttcgtggc
    23041 tttcttatct ggaaaacctg cacagtaaaa ctatcgatct cggccttgag cgcgtgagcc
    23101 aggtcgcggc gcgtcttggc gtcctgaaac cagcgccatt tgtgtttacc gttgcgggta
    23161 cgaatggcaa aggcaccacc tgccgtacgc tggagtcgat tctgatggcg gcagggtaca
    23221 aagtgggcgt ctacagttca ccgcatctgg tgcgttatac cgagcgcgta cgtgtgcagg
    23281 ggcaggaatt gccggaatcg gcccacaccg cctcttttgc ggagattgaa tcggcacgcg
    23341 gtgatatttc cctgacctat ttcgagtacg gtacgctgtc ggcgttgtgg ctgttcaaac
    23401 aggcacaact tgacgtagtg attctggaag tagggctggg cggtcgtctg gacgcaacca
    23461 atattgtcga tgccgatgtg gctgtagtaa ccagcattgc gctggatcat accgactggc
    23521 tgggaccaga tcgcgaaagt attggtcgcg agaaagcagg catctttcgc agcgcaaaac
    23581 cggcaattgt cggtgagccg gaaatgcctt ctaccattgc tgatgtggcg caggaaaaag
    23641 gtgcactgtt acaacgtcgg ggcgttgagt ggaactattc cgtcaccgat catgactggg
    23701 cgtttagcga tgctcacggc acgctggaaa atctgccgtt gccgcttgtc ccgcaaccga
    23761 atgccgcaac agcgctggcg gcactgcgtg ccagcgggct ggaagtcagt gaaaatgcca
    23821 ttcgcgacgg gattgccagc gcaattttgc cgggacgttt ccagattgtg agcgagtcgc
    23881 cacgcgttat ttttgatgtc gcgcataatc cacatgcggc ggaatatctc accgggcgta
    23941 tgaaagcgct accgaaaaac gggcgcgtgc tggcggttat cggtatgcta catgataaag
    24001 atattgccgg aactctggcc tggttgaaaa gcgtggttga tgactggtat tgtgcgccac
    24061 tggaagggcc gcgcggtgcc acggcagaac aactgcttga gcatttgggt aacggcaaat
    24121 catttgatag cgttgcgcag gcatgggatg ccgcaatggc ggacgctaaa gcggaagaca
    24181 ccgtgctggt gtgtggttct ttccacacgg tcgcacatgt catggaagtg attgacgcga
    24241 ggagaagcgg tggcaagtaa gtttcagaat cggttagtgg gcacgatcgt gctggtggcg
    24301 ctgggggtga ttgtacttcc agggctgctg gacgggcaga aaaaacatta tcaggatgag
    24361 tttgcggcta tcccgctggt tccaaaagcg ggcgatcgcg atgagcctga tatgatgcca
    24421 gccgccaccc aggcgttgcc gacgcagccg ccggaaggcg cagcggaaga ggtgcgggca
    24481 ggtgatgccg cagcaccgtc gctcgatccg gccactattg cagccaataa caccgagttt
    24541 gaaccggaac ctgcaccggt cgccccaccg aagccgaaac cggtggagcc gcctaaaccg
    24601 aaggttgaag caccacctgc gccgaagcca gaaccgaagc cggtcgtgga agaaaaagca
    24661 gcgccaacgg gtaaagccta tgttgtgcaa ctgggtgcgc tgaaaaatgc cgataaagtg
    24721 aatgagattg tcggtaagct gcgcggtgcc ggttatcggg tttatacgtc gccatccacg
    24781 ccagtgcagg gtaaaattac ccgtattctg gttgggccgg atgcctcgaa agataagctg
    24841 aaaggttcgc tgggtgagtt gaagcaactt tctggcttaa gtggcgtggt aatgggctat
    24901 acgccgaatt aatacagtct tgcctgatgc gacgctggcg cgtcttatca ggcctgggca
    24961 gtgttagaac cgtaggtcgg ataaggcgtt tacgccgcat ccgacacgca ttgcacgatg
    25021 ccgcaaaggc ataaaaagtc gatggcgttg aatatttttt cagcgccatt tttattgatg
    25081 cgtgggaagg aaatccctac gcaaacgttt tctttttctg ttagaatgcg ccccgaacag
    25141 gatgacaggg cgtaaaatcg tgggacacat atggtctgga ttgattacgc cataatcgcg
    25201 gtgattgctt tttcctctct ggttagcctg atccgcggct ttgttcgtga agcgttatcg
    25261 ctggtgacat ggggttgtgc tttctttgtt gccagtcatt actacactta cctgtcagtc
    25321 tggtttacgg gctttgaaga cgaactggtt cgaaatggaa ttgccatcgc ggtactgttt
    25381 atcgctaccc tgattgttgg tgctatcgtg aacttcgtga taggccagtt ggtggagaaa
    25441 acggggttgt caggcaccga tcgggtgctg ggcgtctgtt tcggtgcgtt gcgcggtgtg
    25501 ttgattgttg ccgccattct cttctttctc gactccttta ccggggtgtc gaaaagcgaa
    25561 gactggagca aatcacagct gatcccgcag ttcagtttta tcatcagatg gttttttgat
    25621 tatctgcaaa gctcgtcaag tttcttgccc agagcgtaag tgctctgaga tgtggcttaa
    25681 cgaggaaaaa gacgtatgtg cggtattgtc ggtatcgccg gtgttatgcc ggttaaccag
    25741 tcgatttatg atgccttaac ggtgcttcag catcgcggtc aggatgccgc cggcatcatc
    25801 accatagatg ccaataactg cttccgtttg cgtaaagcga acgggctggt gagcgatgta
    25861 tttgaagctc gccatatgca gcgtttgcag ggcaatatgg gcattggtca tgtgcgttac
    25921 cctacggctg gcagctccag cgcctctgaa gcgcagccgt tttacgttaa ctccccgtat
    25981 ggcattacgc ttgcccacaa cggcaatctg accaacgctc acgagttgcg taaaaaactg
    26041 tttgaagaaa aacgccgcca catcaacacc acttccgact cggaaattct gcttaatatc
    26101 ttcgctagcg agctggacaa cttccgccac tacccgctgg aagccgacaa tattttcgct
    26161 gccattgctg ccacaaaccg cttaatccgc ggcgcatatg cctgtgtggc gatgattatc
    26221 ggccacggta tggttgcttt ccgcgatcca aacgggattc gtccgctggt actgggaaaa
    26281 cgtgatattg acgagaaccg tacagaatat atggtcgctt ccgaaagcgt agcgctcgat
    26341 acgctgggct ttgatttcct gcgtgacgtc gcgccgggcg aagcgattta catcactgaa
    26401 gaagggcagt tgtttacccg tcaatgtgct gacaatccgg tcagcaatcc gtgcctgttt
    26461 gagtatgtat actttgctcg cccggactcg ttcatcgaca aaatttccgt ttacagcgcg
    26521 cgtgtgaata tgggcacgaa actgggcgag aaaattgccc gcgaatggga agatctggat
    26581 atcgacgtgg tgatcccgat cccagaaacc tcgtgtgata tcgcgctgga aattgctcgt
    26641 attctgggca aaccgtaccg ccagggcttc gttaaaaacc gctatgttgg ccgcaccttt
    26701 atcatgccgg gccagcagct gcgtcgtaag tccgtgcgcc gtaaactgaa tgccaaccgc
    26761 gccgagttcc gcgataaaaa cgtcctgctg gtcgacgact ccatcgtccg tggcaccact
    26821 tctgagcaga ttatcgagat ggcacgcgaa gccggagcga agaaagtgta cctcgcttct
    26881 gcggcaccgg aaattcgctt cccgaacgtt tatggtattg atatgccgag cgccacggaa
    26941 ctgatcgctc acggtcgcga agttgatgaa attcgccaga tcatcggtgc tgacgggttg
    27001 attttccagg atctgaacga tctgatcgac gccgttcgcg ctgaaaatcc ggatatccag
    27061 cagtttgaat gctcggtgtt caacggcgtc tacgtcacca aagatgttga tcagggctac
    27121 ctcgatttcc tcgatacgtt acgtaatgat gacgccaaag cagtgcaacg tcagaacgaa
    27181 gtggaaaatc tcgaaatgca taacgaagga tgatgccttc gctgagggtg ccggtctggc
    27241 accctgactt gcaactcccg ccgaaatact gcaaagtctg cctgcaagtc tgacagggca
    27301 actatttatg aaacgactca ttgtaggcat cagcggtgcc agcggcgcga tttatggcgt
    27361 gcgcttatta caggttctgc gcgatgtcac agatatcgaa acgcatctgg tgatgagcca
    27421 ggcggcgcgc cagaccttat ccctcgaaac ggatttttcc ctgcgcgaag tgcaggcatt
    27481 agctgatgtc acgcacgatg cgcgcgatat ttccgccagc atctcttccg gttctttcca
    27541 gacgctgggg atggtgattt taccctgttc aatcaaaacc ctttccggca ttgtccatag
    27601 ctatactgat ggcttactga cccgtgcggc agatgtggtg ctgaaagagc gtcgcccgtt
    27661 ggtgctctgc gtgcgtgaaa caccattgca cttaggccat ctgcgtttaa tgactcaggc
    27721 ggcagaaatc ggtgcggtga ttatgcctcc cgttccggcg ttttatcatc gcccgcaatc
    27781 ccttgatgat gtgataaatc agacggttaa tcgtgttctt gaccagtttg caataaccct
    27841 tcctgaagat ctctttgccc gctggcaggg cgcataactc cgctgttgcc ctgtttcagg
    27901 gcaattttgc aaccgcgatc aaatcctcga cattttgttt ctgccattca atcgaaacgc
    27961 tgcgattcaa ccgctatacc tgctatcttc aacttcagga caataatgca acgtcttatt
    28021 aacatattta acgttgaatg ttactgttgt cgtcaagatg gcataagacc tgcatgaaag
    28081 agcctgcaaa cacacaacac aatacacaac ataaaaaagc cattttcact tgagggttat
    28141 gtatgaagaa gtcgattctc gctctgtctt tgttagtcgg tctctccgca gcggcttcca
    28201 gctatgcggc gctaccggag acggtacgta tcggaaccga taccacctat gcgccgttct
    28261 cgtcgaaaga tgctaaaggt gattttgttg gctttgatat cgatctcggt aacgagatgt
    28321 gcaaacggat gcaggtgaaa tgtacctggg ttgccagtga ctttgacgcg ctgatcccct
    28381 cactgaaagc gaaaaaaatc gacgccatta tttcgtcgct ttccattacc gataaacgtc
    28441 agcaggagat tgccttctcc gacaagctgt acgccgcaga ttctcgtttg attgctgcca
    28501 aaggttcacc gattcagcca acgctggatt cactgaaagg taaacatgtt ggtgtgctgc
    28561 agggatcaac ccaggaagct tacgctaacg agacctggcg tagcaaaggc gtggatgtgg
    28621 tggcctatgc caaccaggat ttggtctatt ccgatctggc tgcaggacgt ctggatgctg
    28681 cgttacaaga tgaagttgct gccagcgaag gattcctcaa gcaacctgct ggtaaagctt
    28741 tcgcctttgc tggctcatca gtaaaagaca aaaaatactt cggtgatggc accggtgtag
    28801 ggctacgtaa agatgatgct gaactgacgg ctgccttcaa taaggcgctt ggcgagctgc
    28861 gtcaggacgg cacctacgac aagatggcga aaaagtattt cgactttaat gtctacggtg
    28921 actgatacgt cgctgggaag ctgtacctga tggaatgatc atcatggtgc acgccaggtt
    28981 tgttgcacta tcgtggtgca ttgaaatgca tacttaagca tttttaatga aaaataatac
    29041 gtctaacggg gcggaatatt ttgccttgat ggtcaatttt atggcacgat aagtgtaaca
    29101 aacctgtaaa tattccctat aaaaagactg tcagttgagg acattatgaa aaaactggtg
    29161 ctatcgctct ctctggttct ggccttctcc agcgcaactg cggcatttgc tgcgattccg
    29221 caaaacatcc gcatcggtac cgacccgacc tatgcgccat ttgaatcaaa aaattcacaa
    29281 ggcgaactgg ttggcttcga tatcgatctg gcaaaggaat tatgcaaacg catcaatacg
    29341 caatgtacgt ttgtcgaaaa tccgctggat gcgttaatcc cgtccttaaa agcgaagaag
    29401 attgacgcca tcatgtcatc gctttccatt acggaaaaac gtcagcaaga aatagccttc
    29461 accgacaaac tgtacgctgc cgattctcgt ttggtggtgg cgaaaaattc tgacattcag
    29521 ccgacagtcg agtcgctgaa aggcaaacgg gtaggcgtat tgcagggcac cacccaggag
    29581 acgttcggta atgaacattg ggcaccaaaa ggcattgaaa tcgtctcgta tcaggggcag
    29641 gacaacattt attctgacct gactgccgga cgtattgatg ccgcgttcca ggatgaggtc
    29701 gctgccagcg aaggtttcct caaacaacct gtcggtaaag attacaaatt cggtggcccg
    29761 tctgttaaag atgaaaaact gtttggcgta gggaccggca tgggcctgcg taaagaagat
    29821 aacgaactgc gcgaagcact gaacaaagcc tttgccgaaa tgcgcgctga cggtacttac
    29881 gagaaattag cgaaaaagta cttcgatttt gatgtttatg gtggctaatc gccatcggtg
    29941 aaagaaagcc cgctcccttc gggtaaccgg agggagacaa gaggcggtaa ttcaccacac
    30001 acgacaggac aggcagcatg ttgtatgggt tttcaggtgt tattttacag ggtgcgctcg
    30061 tcacgctgga gctggctatc agctctgtag tgctcgctgt aatcatcggt ttaattggcg
    30121 ctggcggtaa gctctcgcaa aatcggcttt cggggctgat tttcgaaggg tacaccacgc
    30181 tgattcgtgg cgtgccggac ttagtgttga tgctgctgat tttctacggt ttgcagattg
    30241 cgctaaacac ggtgacggag gcgatgggcg tcgggcagat tgatatcgat ccgatggtcg
    30301 ctggtattat cactctcggt tttatctacg gtgcttattt taccgaaacg ttccgtggtg
    30361 cttttatggc agtgccgaaa ggacatatag aggcggcgac ggcgttcggt tttactcgtg
    30421 ggcaagtgtt tcggcggatc atgtttccgg cgatgatgcg ttacgccctg ccaggcattg
    30481 gcaacaactg gcaggtgatc ctcaaatcta ccgcactggt ttcgttactc ggcctggaag
    30541 atgtggtcaa agccacccaa ctggcaggca aaagtacctg ggaaccgttc tatttcgcca
    30601 tcgtctgtgg cgtgatttac ctggttttca ccactgtttc caatggtgtg ctgctgttcc
    30661 ttgagcgccg ctactccgtg ggtgtgaaga gggctgacct gtgatcgaaa tcttacatga
    30721 atactggaaa ccgctgctgt ggaccgacgg ttatcgcttt actggtgtgg cgatcactct
    30781 gtggctgctt attttgtcgg tagtgatagg cggagtcctg gcgctgtttc tggcgattgg
    30841 tcgtgtctcc agtaataaat acatccagtt tccaatctgg ttatttacct atatttttcg
    30901 cggtacgccg ctgtatgttc agttgctggt gttctattcc ggcatgtaca cgcttgagat
    30961 tgttaaggga accgaattcc ttaacgcttt cttccgcagt ggcctgaact gtaccgtgct
    31021 ggcgctgacg cttaacacct gcgcttacac taccgagatt tttgctgggg caatccgttc
    31081 ggttccgcat ggggaaattg aagccgccag agcctatggc ttctcgactt ttaaaatgta
    31141 tcgttgcatt attttgcctt ctgcgctgcg tattgcgtta ccggcataca gcaacgaagt
    31201 gatcctgatg ctgcactcta ctgcgctggc gtttacggcc actgtgccgg atctgctgaa
    31261 aatagcccgc gatattaacg ccgccacgta tcaacctttt accgccttcg gcattgccgc
    31321 ggtgctctat ttaatcatct cttatgtcct gatcagcctc tttcgcagag cggaaaaacg
    31381 ctggttgcag catgtgaaac cttcttcaac gcactgagaa tacgatgtcc gagaataaat
    31441 taaacgttat cgatttgcac aaacgctacg gcgaacatga agtgctgaaa ggggtctcac
    31501 tgcaagcgaa tgccggagat gtaataagca tcatcggatc gtcgggatcg gggaaaagta
    31561 cctttctgcg ctgcattaac ttcctcgaaa aaccgagtga agggtcgatc gtggtcaatg
    31621 gccagacgat caatctggtg cgcgacaaag acggtcaact caaagtcgcc gataaaaatc
    31681 aactgcgctt actgcgcaca cgcctgacga tggtattcca gcacttcaat ctctggagcc
    31741 atatgacggt gctggaaaac gtcatggaag cgccgattca ggtgttgggc ctgagcaagc
    31801 aggaagcgcg cgagcgggcg gtgaagtatc tggcgaaagt cgggatagac gaacgtgcgc
    31861 aggggaaata tccggtgcat ctttccggcg gtcagcaaca gcgtgtttct atcgcgcggg
    31921 cgctggcgat ggaaccggaa gttttactgt ttgatgaacc tacctcggcg ctcgatcctg
    31981 aactggtagg cgaagtgtta cgtattatgc agcaactggc agaagagggg aaaaccatgg
    32041 tggtagtgac tcacgaaatg ggctttgctc gccatgtttc tactcacgtc attttcctcc
    32101 atcaggggaa aatagaagaa gagggcgcgc cggagcagtt atttggcaac ccgcaaagcc
    32161 ctcgtctgca acagttcctt aagggatcgc tgaaataatt tcctctccgg tggctaacgc
    32221 tgccggagta ttttcatggg gaatttgcga gtcagcttcc gctgaaatgt aaaagttgca
    32281 gtccgctaaa aatacttctg aattcgctgt ccaaagataa aaactgcctg ctgcattggg
    32341 tgttaatcgt ggttattgtc gcgtcatctt aaatttgagg ggtgacggaa cgccatgaca
    32401 atatcgacaa cttccacgcc gcatgatgcg gtatttaaat cttttttacg ccatccag
//
LOCUS       DADMKF010000033        31793 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.034, whole genome shotgun sequence.
ACCESSION   DADMKF010000033 DADMKF010000000
VERSION     DADMKF010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31793
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.034"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            <1..1132
                     /locus_tag="JNC24_001395"
     CDS             <1..1132
                     /locus_tag="JNC24_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAY9158185.1"
                     /translation="LVQSVEGEKEASAARQTKLALDIANKTLPLFRHVNSESLRKVCE
                     IIRDDIHADAVAITNTDHVLAYVGVGEHNYQNGDDFISPTTRQAMNYGKIIIKNNDEA
                     HRTPEIHSMLVIPLWEKGVVTGTLKIYYCHAHQITSSLQEMAVGLSQIISTQLEVSRA
                     EQLREMANKAELRALQSKINPHFLFNALNAISSSIRLNPDTARQLIFNLSRYLRYNIE
                     LKDDEQIDIKKELYQIKDYIAIEQARFGDKLTVIYDIDEEVNCCIPSLLIQPLVENAI
                     VHGIQPCKGKGVVTISVAECGNRVRIAVRDTGHGIDPKVIERVEANEMPGNKIGLLNV
                     HHRVKLLYGEGLHIRRLEPGTEIAFYIPNQRTPVASQATLLL"
     gene            1147..1881
                     /locus_tag="JNC24_001396"
     CDS             1147..1881
                     /locus_tag="JNC24_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAY9158186.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            1894..2751
                     /locus_tag="JNC24_001397"
     CDS             1894..2751
                     /locus_tag="JNC24_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY9158187.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRMVEGEMLFIPARAANLPINNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQIQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYAQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFGLTP
                     GEYSARFQG"
     gene            complement(2754..5249)
                     /gene="ptsP"
                     /locus_tag="JNC24_001398"
                     /pseudo
     CDS             complement(2754..5249)
                     /gene="ptsP"
                     /locus_tag="JNC24_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
     gene            complement(5274..6311)
                     /gene="ypdE"
                     /locus_tag="JNC24_001399"
     CDS             complement(5274..6311)
                     /gene="ypdE"
                     /locus_tag="JNC24_001399"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAY9158188.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEADRLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGARSYDEVMQAGIRPGDRVTFDTAFQVLPHQRV
                     MGKAFDDRLGCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVSAEIGVPLQAD
                     MFSNGGTDGGAVHLTDTGVPTVVMGPATRHGHCAASIADCRDILQMEQLLSALIQRLT
                     RETVVQLTDFR"
     gene            complement(6311..7396)
                     /gene="ypdF"
                     /locus_tag="JNC24_001400"
                     /pseudo
     CDS             complement(6311..7396)
                     /gene="ypdF"
                     /locus_tag="JNC24_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
     gene            complement(7411..8658)
                     /locus_tag="JNC24_001401"
     CDS             complement(7411..8658)
                     /locus_tag="JNC24_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-like permease IIC component"
                     /protein_id="HAY9158189.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAVI
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(8680..9006)
                     /locus_tag="JNC24_001402"
     CDS             complement(8680..9006)
                     /locus_tag="JNC24_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-like phosphotransferase enzyme IIB
                     component 1"
                     /protein_id="HAY9158190.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(9226..10191)
                     /gene="glk"
                     /locus_tag="JNC24_001403"
     CDS             complement(9226..10191)
                     /gene="glk"
                     /locus_tag="JNC24_001403"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HAY9158191.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALTLGTFGGVYIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDNPGLLGSGAHLRQTLGHIL"
     gene            10395..11651
                     /locus_tag="JNC24_001404"
     CDS             10395..11651
                     /locus_tag="JNC24_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HAY9158192.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPIWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMNQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            11766..12092
                     /locus_tag="JNC24_001405"
     CDS             11766..12092
                     /locus_tag="JNC24_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HAY9158193.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(12233..13471)
                     /gene="mntH"
                     /locus_tag="JNC24_001406"
     CDS             complement(12233..13471)
                     /gene="mntH"
                     /locus_tag="JNC24_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mn(2+) uptake NRAMP transporter MntH"
                     /protein_id="HAY9158194.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            13807..15009
                     /gene="nupC"
                     /locus_tag="JNC24_001407"
     CDS             13807..15009
                     /gene="nupC"
                     /locus_tag="JNC24_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HAY9158195.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLRFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(15059..17247)
                     /locus_tag="JNC24_001408"
                     /pseudo
     CDS             complement(15059..17247)
                     /locus_tag="JNC24_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311300.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing phosphodiesterase"
     gene            complement(17456..17531)
                     /locus_tag="JNC24_001409"
     tRNA            complement(17456..17531)
                     /locus_tag="JNC24_001409"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17496..17498),aa:Ala,seq:ggc)
     gene            complement(17571..17646)
                     /locus_tag="JNC24_001410"
     tRNA            complement(17571..17646)
                     /locus_tag="JNC24_001410"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17611..17613),aa:Ala,seq:ggc)
     gene            17882..18226
                     /locus_tag="JNC24_001411"
     CDS             17882..18226
                     /locus_tag="JNC24_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HAY9158196.1"
                     /translation="MTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKARLVHV
                     NEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLLREGII
                     GLLQRLGIRDSK"
     gene            18249..18620
                     /locus_tag="JNC24_001412"
     CDS             18249..18620
                     /locus_tag="JNC24_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HAY9158197.1"
                     /translation="MTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGGRARLILV
                     DTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTDIEQGKVA
                     QFLSREGIRNFLARLDIDESA"
     gene            complement(18672..20087)
                     /gene="gltX"
                     /locus_tag="JNC24_001413"
     CDS             complement(18672..20087)
                     /gene="gltX"
                     /locus_tag="JNC24_001413"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HAY9158198.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALDFIAERENQQ"
     gene            20346..20421
                     /locus_tag="JNC24_001414"
     tRNA            20346..20421
                     /locus_tag="JNC24_001414"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20379..20381,aa:Val,seq:tac)
     gene            20467..20542
                     /locus_tag="JNC24_001415"
     tRNA            20467..20542
                     /locus_tag="JNC24_001415"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20500..20502,aa:Val,seq:tac)
     gene            20589..20664
                     /locus_tag="JNC24_001416"
     tRNA            20589..20664
                     /locus_tag="JNC24_001416"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20622..20624,aa:Val,seq:tac)
     gene            20669..20744
                     /locus_tag="JNC24_001417"
     tRNA            20669..20744
                     /locus_tag="JNC24_001417"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20702..20704,aa:Lys,seq:ttt)
     gene            20775..20900
                     /locus_tag="JNC24_001418"
     ncRNA           20775..20900
                     /ncRNA_class="other"
                     /locus_tag="JNC24_001418"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            21020..21361
                     /locus_tag="JNC24_001419"
     CDS             21020..21361
                     /locus_tag="JNC24_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flxA-like family protein"
                     /protein_id="HAY9158199.1"
                     /translation="MATISSTSIPSIQTQSSNRASQGSDVASKIARISQQIIKLTQQI
                     KEIVDTSGSAEDKQKQAELIQQQITLLETQLAQLQRQQAEKAQEKEQRLSLNVSLLNP
                     VENTTHIDIYI"
     gene            complement(21352..22278)
                     /locus_tag="JNC24_001420"
     CDS             complement(21352..22278)
                     /locus_tag="JNC24_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY9158200.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFCIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGAPLYQI"
     gene            22368..23366
                     /locus_tag="JNC24_001421"
     CDS             22368..23366
                     /locus_tag="JNC24_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HAY9158201.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGAGGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLGFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(23363..23581)
                     /locus_tag="JNC24_001422"
     CDS             complement(23363..23581)
                     /locus_tag="JNC24_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9158202.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(23583..25598)
                     /gene="ligA"
                     /locus_tag="JNC24_001423"
     CDS             complement(23583..25598)
                     /gene="ligA"
                     /locus_tag="JNC24_001423"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HAY9158203.1"
                     /translation="MESIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGLPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKSAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEVLEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            complement(25669..26655)
                     /gene="zipA"
                     /locus_tag="JNC24_001424"
     CDS             complement(25669..26655)
                     /gene="zipA"
                     /locus_tag="JNC24_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HAY9158204.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYQPPYASAQPR
                     QPIQQPPEAQVPPQHAPRPAQPMQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSAPQPA
                     QQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLNSIQQ
                     AGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQVPSYG
                     DELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            26885..27646
                     /gene="cysZ"
                     /locus_tag="JNC24_001425"
     CDS             26885..27646
                     /gene="cysZ"
                     /locus_tag="JNC24_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HAY9158205.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTLMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFAWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            27831..28802
                     /gene="cysK"
                     /locus_tag="JNC24_001426"
     CDS             27831..28802
                     /gene="cysK"
                     /locus_tag="JNC24_001426"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HAY9158206.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQALAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            29186..29443
                     /gene="ptsH"
                     /locus_tag="JNC24_001427"
     CDS             29186..29443
                     /gene="ptsH"
                     /locus_tag="JNC24_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HAY9158207.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            29488..31215
                     /gene="ptsI"
                     /locus_tag="JNC24_001428"
     CDS             29488..31215
                     /gene="ptsI"
                     /locus_tag="JNC24_001428"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HAY9158208.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            31256..31765
                     /gene="crr"
                     /locus_tag="JNC24_001429"
     CDS             31256..31765
                     /gene="crr"
                     /locus_tag="JNC24_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAY9158209.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
BASE COUNT         7843 a         8383 c         7906 g         7661 t
ORIGIN      
        1 tctggtgcaa agcgttgagg gcgaaaaaga ggccagcgcc gcgcggcaga ccaagctggc
       61 gctggatatc gccaacaaaa cgctaccgct gtttcgccat gtcaatagcg agtcattacg
      121 caaggtctgc gaaattatcc gcgatgacat tcacgccgat gcggtggcga ttactaatac
      181 cgatcatgtg ctggcctatg ttggcgtggg tgaacataac tatcagaatg gcgatgactt
      241 cattagcccg actacccgtc aggcgatgaa ttacggaaaa atcatcatta aaaacaatga
      301 tgaagcccac cgcacaccag agattcattc catgctggtg atcccattgt gggagaaagg
      361 ggtcgtgacc ggaacgctga aaatttacta ctgccacgcg catcagatca cctcgtcatt
      421 acaggaaatg gcggtcggtc tgtcgcaaat catctccacg caactggagg tttcacgcgc
      481 cgagcagcta cgtgaaatgg caaataaggc agagcttcgc gccctgcaaa gcaaaattaa
      541 tccccatttt ctgtttaacg ctctgaacgc tatttcatcg tcaatccgtc tgaatccgga
      601 taccgctcgc cagttgatct ttaatctgtc gcgttatctg cgctataaca ttgaattaaa
      661 agacgatgag caaatcgata tcaaaaaaga gctgtatcaa attaaagact atattgccat
      721 tgagcaggcc cgctttggtg acaagctgac ggttatctat gatattgatg aagaggtgaa
      781 ttgctgcatt cccagcctgc tgatccagcc gttggtggag aacgccattg tccacggtat
      841 tcagccttgc aaaggtaaag gcgttgtcac catcagcgtt gcagagtgcg gaaatcgggt
      901 acgcattgcg gtgcgagata ccgggcacgg catcgatcca aaggtgattg agcgggtcga
      961 agcgaatgaa atgccgggca ataaaattgg cctgctgaat gtccatcatc gcgtgaagtt
     1021 attgtatggc gaggggctgc atatccgccg cctggagccg gggacggaaa ttgcgtttta
     1081 cattcctaac caacgcaccc cagtcgcctc acaggctacg ttattgcttt gagccggagt
     1141 gatattgtga aagtcatcat tgttgaagac gaattcctgg cacaacagga actgagctgg
     1201 ctaattaaag agcacagcca gatggagatt gtcggcacct ttgacgacgg tctggacgtg
     1261 ttgaagtttt tgcagcataa ccgcgtcgac gccatttttc tggatatcaa tattccgtcg
     1321 ctggatggcg tgttgctggc gcaaaacatc agccagttcg cccataaacc gtttattgtg
     1381 ttcatcaccg cgtggaaaga acatgcggta gaagcgtttg aactggaggc gtttgactac
     1441 attctcaaac cgtatcagga gtcacgtatt accgggatgc tgcaaaaact ggaagcggcc
     1501 tggcaacaac agcagaccag cagtacgcct gccgcgacgg taacgcgtga gaatgacacc
     1561 attaatctgg tgaaagatga gcgaataatc gtcacgccaa ttaacgatat ctattacgcc
     1621 gaagcgcacg agaaaatgac ctttgtctat acgcggcgtg aatcctacgt aatgccgatg
     1681 aacattaccg aattttgcag caaactgccg ccgtcgcatt ttttccgctg ccatcgctca
     1741 ttttgtgtca atctgaacaa aatacgcgaa atcgaaccgt ggtttaataa cacctacatt
     1801 ctgcgactga aagatctgga ttttgaagtg ccggtcagcc gcagcaaagt gaaagaattt
     1861 cgccagttaa tgcatcttta atcgggaact ttcatgaaag caccaggctt gcccgccgat
     1921 caacaatttt tcgccgatct gttcagcggc ctggtgctta acccgcaact actggggcgc
     1981 gtctggtttg ccagccagcc tgcctcgtta ccggtgggca gtttatgtat tgattttccc
     2041 cgtctggata tcgtgctgcg cggcgaatac ggcaacctgc tggaagcaaa gcagcagcga
     2101 atggtggaag gagaaatgct gtttattccg gcacgcgcgg ctaatttacc gatcaacaac
     2161 aaaccggtga tgctgttaag cctggtgttc gcaccgacct ggcttgggtt atcgttttac
     2221 gatagccgca ccacgtcgtt gttgcatcct gctcgccaga tccagcttcc cagcctgcaa
     2281 cgcggtgaag gtgaagcgat gcttaccgcc ctcacccatc ttagccgttc gccgctggag
     2341 cagaatatca ttcagccact ggtgttaagt ttgctgcatc tttgccgcaa cgtggtgaat
     2401 atgccgccgg gtaattcgca accgcgcggc gattttctct atcacagcat ttgtaactgg
     2461 gttcaggata attatgccca gccgctcacc cgcgagagcg tggcgcagtt ttttaatatc
     2521 acgcccaatc atctgtcaaa actgtttgct cagcatggaa cgatgcgttt tatcgagtat
     2581 gtgcgttggg tgcgaatggc gaaggcgagg atgattttgc agaaatatca tctgtcaatt
     2641 catgaagtgg cacagcgttg cggttttccg gatagcgact atttttgtcg cgttttccgg
     2701 cgtcagtttg gtctgacgcc gggagagtac agcgcccgtt ttcagggcta acgtcagaag
     2761 gttaattctg tttccagcag cgtcaggata ctttgtgcat cttgcgcggc aaacaacgac
     2821 tggcggaagt ttttattcac cagtttacgc gccagctgcg agaagacttt cacatgattc
     2881 atcccttcgt tagcacccag cgtcagcatg atcaccagtt caacttcgcc catttctgac
     2941 tgccagtcaa ccggtttcgc cagccgggca atgctgatac tggaatgacg gatccactga
     3001 gatttggtgt gcgggatcgc tacgccaaaa ccaacgccgg tggtaacaat ctcttcccgc
     3061 tgccagacat cttcttccag ctcaaacgga tgttcagtgc gcccgttaac gccgaggttg
     3121 ccgcacagga actggatcgc ctgctcttta ttgctaaaat cctgatcaac aaagatattc
     3181 tccagcgcca gcagtgggcg aacgtcttct tccggcgtaa aggcggtgag taacgcttca
     3241 atttcctgcg cactgcggca ttcacatgcc tgacgcgcca gttcccgaca cgcctcgcta
     3301 tccagttgac gaagctggct tttcaccgcc ggaatacgcg ggctactcat actcagctca
     3361 tccaggccca gcccaagcag tagcggcaga taacggcttt caccgcccag ttcaccgcaa
     3421 atgcctaccc atttgccccg ctgatgcgca gtggtaacta tttgctgcaa catgcgcagg
     3481 aacgatggcg taatcgggtt atatagcggc gatacgcgcg ggttattacg atcgaccgca
     3541 tacagatact gggtcatatc gttggagccg atactgaaga aatcgacctc atcgcagaag
     3601 tggtcgatga tgtagcacac cgacggcact tccaccatga tcccaagcgt aatcgtctct
     3661 gcatgacgca ggccatcgcg cttaagctca acgatcgctt tttggatctc gcctttcacc
     3721 cataagatct gatcgaggct gttaaccatc gggatcatca actgggcgtt gccgaaactg
     3781 gcggcgcgca aaatggcccg cagttgagtg cggaacaggc cagcaaattc cgggtaaata
     3841 cgtaccgcgc gatagccgag gaacgggttc tcttcctggg gaatattcag ataaggaatg
     3901 cttttatcgc cgccgatgtc catcgtgcgg aagataatcg gcttgtcgcc cgccgccagt
     3961 agcacctgct ggtaggcttc aaattgctcc tgctcgtccg gcgcgctgtc gcgatccata
     4021 tagagcattt cggtacggaa cagcccgaca ccttccgcgc cgttggcaaa cgcgcctggc
     4081 gcttccagag cggtgccgat attcgccgca atatcaatac gcttgttatc acgggaatag
     4141 gccagctgcg cggcagcctg cgcctgttgt ttttggcgtt tatccgccag cgtctgcgcg
     4201 acctgataat aaccgctcac cgcgtcattc gggttaatcg ccagcacgcc gcactgggcg
     4261 tcaagcactg caggttgccc ggcataacgg gcaatcgcat ccagcggcaa gccactcagt
     4321 accgggatcg ccgaggcacg ggccagaatc agtgtatgcg aggtgcgccc ggttttctcc
     4381 agaatcatgc ccgcaagatt tttcaaatca aggctcaaaa actggcttgg ggttaaatct
     4441 tcagccacca gaatggtcgg tttttccagc accagattgt tgcgcggctt cagttccggc
     4501 taggtgatat gcagcaactg ttcgctgata tcgcgaatgt cgctgacccg ctcgcgcaga
     4561 taatcgctgg cagaagcaga tagtttggcg caaacctgct ccatattgct gatgatcgcc
     4621 gcccccagtc cctgatgctg ttctgtcatc aggcgacgga tattgcctgc aaattcatca
     4681 tcctgaatca gcgacaaatg agcgctgagg atagttttgc tttcgccgtc acgctcacgc
     4741 aattgctggt tcagttgctc ggcaagcgtt gccaggctgt gctccagccg ggtggaatct
     4801 tgcgcactgg cggggattgc ccgataactg tcgaggctgt cgctttgtaa cagggtcagc
     4861 gtacccacgc cgacgccgct tgccagcaca ttgccgtaca gtaaatccgg gttaaggcgg
     4921 cttaatgaac gcggcagcgg atgcgccgtc agttctgcct gcgtcggctg aacgctgtcg
     4981 ctgtcgataa agcgcaccag gatgtactct tccagcacgc gccgcgcctg ctcttcatcg
     5041 ctgccgctaa tgttcaggct gcaactgtca ttaaataagg tgccagttcc aatcaacgcc
     5101 agcgagcttt tagcatcggc ctttgcgttc tggcgatggt tgataaaggt gatttcgctt
     5161 tgccactggc tgcactgttc tttaagttcc cacgccggac gagcatgtag accgttaggc
     5221 aaaggacaga gaaattgaat cgttaacata accgctccgg gttaatcagg agatcatctg
     5281 aaatccgtca gttgaacaac cgtctcacgc gtaagacgtt gaataagggc agataaaagt
     5341 tgctccatct gcaaaatgtc gcggcaatcg gcaatcgatg ccgcgcaatg tccgtggcgg
     5401 gtggcaggcc ccattaccac tgtgggtacg ccagtatcgg ttaagtgcac cgctccaccg
     5461 tccgtgccgc cgttactgaa catatccgcc tgcaacggaa cgccaatttc tgccgacacg
     5521 gtttcaatcc acgcggtaag ttttggcggc gcaatcagcg atttatcgct taacaccagc
     5581 atcggcccgt taccaatctg acgatggtta gccgcgccat aatcaaagtt tttcgcccag
     5641 caggcggtat caagcacaat ggcgacgtcc ggcgacaccg cgcgggtagc agtttgcccg
     5701 ccgcgtaatc ccacctcttc gctggaactt gccaccagcc acacttccgc aggcagttcg
     5761 gcgtcgtgca gctcacgtag taacgtcacc agcagatagc aaccgaggcg gtcatcaaag
     5821 gctttcccca tcacccgctg gtgagggaga acctgaaaag ctgtatcaaa ggtgacgcga
     5881 tcgcctggac gaattcccgc ctgcatcact tcgtcatacg agcgcgcacc aatgtccacg
     5941 cgcatggcgc tgacgtcatt cccctgccgg tcgccgtcaa gcaggcctgg aattttgcac
     6001 tcttcacggg tggtgatgcg caccggctgc agctggcggg cagccatgcg cacgttgcca
     6061 accggcagca catcaattgc cccttcgcgg gagatgctgc gcaccataaa tcccacttcg
     6121 tccatatgcg cacagatcat caccttcgga cctgtcgatt cattgaggcg gatcagcacc
     6181 gatcccagac catcaaatcg tacttctttt tgcaggcgat ccgcttcttc cagtaggatc
     6241 tgccgcactt cctgttccga ggaggcgatc gcatctgcct cgctcaacgc ttttaatagc
     6301 gataaatcca ttatgcctct cccgtgagca acactgtttt cggcatggcg tagagcactt
     6361 ctgcgccttg cggggtaacc agcacaacat cttcgatgcg cacgccccct tgccctggca
     6421 aataaatccc cggctccacg gtcagtaaca tgcctggctg tagcgtcgtg gtatcccgcg
     6481 gtgaaaagcg tggatcttcg tgtacttcaa taccgatagc atgaccggtg ttgtgaccaa
     6541 aatagtcgcc aaatcctgcc tcggtaatca cccggcgcgc ggcgtcgtca acctgttagc
     6601 agcgtacgcc agggcgaatc gcggagattg ccgccagctg tgcttgcagg acaatctgat
     6661 agacgttaaa cagcgggtga gattcggcaa ttaccccttc gccattcacc agcaaggtgc
     6721 gcgtcatatc agagcagtac ccctgataga gcgcaccaaa atcaagagtg acaaactcgc
     6781 ctgctgcaac aatcttgtcg ctggctttgc cgtgcggcag cgccccacgc cagccgctgg
     6841 cgacaatggt gtcaaaagag gctttttctg cgccctgctg gcgcataaac cactccagtt
     6901 cagcggctat ctcgcgctcg ctcatccccg cctgaataaa gcggcgaata tgctccgctc
     6961 cgcgatcggc aatcccacag gcgaggcgga ttatctccac ctcctctggc gttttgattt
     7021 gccgcagcac atccggcgtg gcgctaacca gtttcgcatt gagttcagac ttccagcggt
     7081 gcgcggtttc ccaactcacc tgctggcctt caaaaccgag cgtttgcaac tgctcatcgg
     7141 caatgatttg attgacgata gtggtaagcg tgttcgtcgc gtcaagcaaa tgcagctggt
     7201 agccttgcgc acgggcttcc acctcaacga aatagcgcga atctaccaga atgtgcgcac
     7261 tttcacggct aataaccaca tagcctgatc cggtggagat ccccagatgc ggctgtttgt
     7321 tctgccgtga ggagagaagc actgcatcca gttgttgcgc cttaagccag tcgcgcagcg
     7381 aagcgagtaa tgtcatctgg attttccttc ttacaggcta tcgattaaca atttaccttt
     7441 acggaacatc atcagacgca ggaacaccac catcagcgca gttatgactg cccccagcgc
     7501 gatccccgcc atatagacgc ccaggttagt gaccagcggc catgcccaga tagcggattc
     7561 cgggaaccat tgcactgcgc ccagccagac agcggcggtt gagccgacaa tcgcgccgac
     7621 catatacgac ggaatggcgg tgatggggct ttccagcgca aacggaatcg caccctcact
     7681 aatccccata aaggcaagga acatcgcggt cttaccctgc ggataaagtt gagcgttgaa
     7741 caggcgtttg ccggttaaac gacggtcgat aatggtcgcc agacccagac ctatcggcgg
     7801 aataacgata gcgatagaac gtgcagtgac cggtaatacg tggtcagtgg taaagctgaa
     7861 ggcaacaaaa cctgcggctt tgttgatcgg gccaccaagg tcgattgcgg tcgccgcggc
     7921 gatccccatt gcgtacatca gcgcgccctt ctcacctgcg gcagtcagca cggtacggat
     7981 accgccgttg atccagccgc caaagggggt aatgacgtag tacatcgcca gcatgacaaa
     8041 aatggcggaa agaatcggta gcaaaaatgt ggttttgaac gctaacagga aatccggtaa
     8101 ctggattttc tggttcatcc acttcacaag gtaacccgcg acgatagaaa taatcagcgc
     8161 accaatgaag gtggacggca ccggcgaaga ggtcgcccat tgcatagtgc tgggatcgaa
     8221 gttcagcagt tgcgtcggct gggtagacat caagccgccg ataaaaccag ccgggaacgc
     8281 cagtttgcca ccgatagagt tcgccacaaa agcagcaaac atcggaatgg caaaaccaaa
     8341 caacacgccg ccgaaggact gcgacagcca ggcaaatttc agtaaggaga ggtcgaaacc
     8401 ggagaatttc ccgctattaa gagcatccat gatgccgatc tcagcaggaa ttttaagcca
     8461 gctataagca atcagctggc tgaaagcgag gatcacaccg cccataatta aggtcggcac
     8521 catgcgtgaa atccctgaca tcacatgctg cgggagttca ccccagaagc tgcttttaga
     8581 ggcctgcgga ttcttaactg ccgctgccgc accggatgcg ccaggcacaa cggttgcact
     8641 gcgtttttta atggccataa caattatccc tgtcgaatat tactgttgtt cagaggcaat
     8701 catctcttcg atttctttga tgatgcccgc agcgttttta attgcgtcct gcaaagtgat
     8761 ttcataaacg tcgcgtgatt cgaaacgctc attatcttcc ggggtaactg ccacggagtg
     8821 gatgatgatg gttgcttcgg cgatatcctg cgccgtcagg cgattctgga taccgtccgc
     8881 gccctgggtt tcaattttca cttcataacc tgcttctacc gccgcttctt ccagcgcctg
     8941 agcggccata aaggtgtgag ccaggcccat tgggcaggca cataaggcaa ttagtttctt
     9001 actcatcgtt aattcctcgt tcagtgatag ttgacgagtg aaattatgaa cgtgcaaaac
     9061 aaatcgccgt taccagacaa ataatgcatt tactggagaa ttaatcccgc tgtacggaag
     9121 tgtgacgcag gtcggccttg tgagggggac acaaagaaaa attgaattaa ctgtatgatt
     9181 taaaagatta tcgggagagt tacctcccga tataacagaa aggatttaca gaatgtgccc
     9241 taaggtctgg cgtaaatgtg caccggaacc gagaagaccc ggattgtcat ggacgatgag
     9301 atacaccgga atatcatgga catattcttt aaagcgccct ttatcttcaa atgcggcacg
     9361 gaaaccagag gctttgaaga actcaaggaa gcgcggcacg ataccgccag caatataaac
     9421 cccaccaaag gttcccagcg ttaatgccag attgccgcca aaacggccca taatgacgca
     9481 aaacagcgac aacgcacggc ggcaatcggt gcagctgtca gccagcgcgc gttcggtaat
     9541 atcttttggc ttgagatttt ctggcaggcg gttgtcggct ttaacaattg cgcgatacaa
     9601 attcaccagc ccaggaccag aaagcacgcg ttccgccgaa acatgaccaa tttccgcacg
     9661 caatatttcg aggataatgg cctcttcttc actattcggc gcaaaatcaa cgtgaccgcc
     9721 ttcgcccggc aagcttaccc aacgcttatc gacatggacc agatgcgcaa ccccaagccc
     9781 tgttccggca ccgtaaaccg caataggttt accttcgacc ggttctgcgc caccaaactg
     9841 aatcagatgc tcttttttca gcatcgggat cgccatcgat acagcggtaa aatcgttaat
     9901 aatttccaga tggctaaaac cgagattctt tttcatttcg gcaattgaga acgcccaggt
     9961 atggttggtc attgccaccc agtcaccggt aattgggcaa gcgatggcaa tacagccgtc
    10021 tttcacctcg accttatgtt cttcaagata aacgcgaatg accgcttcga ggctggggta
    10081 atcaagccct gaataggtct tagcctgcga gatttcacca ctggcaatat cacacagagc
    10141 aagacgtgcg ttggtgccgc ccacatcacc gactaatgca tactttgtca ttcttcaact
    10201 gctccgctaa agtcaaaata attctttctc acactgtaaa tacctggggg cataacaaca
    10261 acgccggaaa ggcaggctcc ctgtaaatat cgatctgggt cacacattta ctttatcgtt
    10321 tcagcaccaa ttgcagcgat gcctttttgc aagctgggca aactaagtat ctgaccccgc
    10381 ataaggaata gaacatgctc catccgcgag ccagaaccat gttgttatta tcgctccccg
    10441 ccgtggcaat tgggattgcg tccagtctta ttctgattgt ggtgatgaaa atcgcctcgg
    10501 tattacagaa tttgctctgg caacgactgc cgggaactct ggggatagcc caggattcac
    10561 ctatctggat catcggtgta ttaacgctaa cgggtattgc ggtggggttg gttatccgtt
    10621 tcagccaggg tcatgccgga ccagaccccg cctgtgaacc gctgatcggc gcaccagttc
    10681 cgccctctgc gctaccagga cttatcgtag cattaattct cggtcttgct ggcggcgtca
    10741 gcctggggcc ggaacatccg atcatgaccg tcaatatcgc ccttgccgtc gccattggcg
    10801 ctcgtctgtt accgcgcgtc aaccgaatgg agtggactat tttagcctct gccggaacca
    10861 tcggcgcgct gtttggtaca cctgttgcgg cggcgttgat attttcgcaa accttaaatg
    10921 gcagtagtga agttccgcta tgggatcgtc tttttgcgcc gttaatggcg gcagcagctg
    10981 gtgcacttac taccggatta tttttccatc cccatttttc actgcccatt gcgcattacg
    11041 gacagatgga aatgaccgat attctcagcg gtgcaattgt cgcggcgatt gccatcgcag
    11101 cagggatggt cgccgtatgg tgcttaccac ggttgcacgc gatgatgaat caaatgaaaa
    11161 atccggtgct cgtgctgggt attggcggat ttattctcgg aattctgggg gttattggtg
    11221 gaccagtttc gctgtttaaa gggctggatg agatgcagca gatggtggca aatcaggctt
    11281 tcagcaccag cgattacttt ttgctggcgg taattaaact tgccgccctg gtcgttgctg
    11341 ccgccagtgg ctttcgcggt gggcgaatct tcccggcagt gtttgtcggc gtggcattag
    11401 ggttgatgct gcatgagcac gttcccgccg ttccagcggc aataaccgtt tcctgcgcta
    11461 ttctcggcat cgtgctggtg gtaacacgcg atggctggtt aagtcttttt atggcggcag
    11521 tcgttgtacc caataccaca ttgctaccgc tgctctgtat cgtcatgctt ccggcatggc
    11581 tgttattagc aggtaagccg atgatgatgg tcaatcgtcc gaagcaacag ccaccccacg
    11641 ataatgttta gcaaaaaatg ctctccttta tgatttaaga gttatggcga tgatacgggg
    11701 ctttacctcc ccgtaatatt gcgttaacag gccgctgacg gcttcgatcg tgaaggagaa
    11761 taacaatgtt caggtcactg tttctggcgg ccgccctgat ggcatttacc ccgcttgcag
    11821 caaatgcagg tgaaatcacc ctactgccat cgatcaaatt acaaattggc gatcgcgatc
    11881 attacggtaa ttactgggac ggtggtcact ggcgagatcg tgattactgg catcgcaatt
    11941 atgaatggcg caaaaaccgc tggtggcgtc atgataatgg ctatcatcgt ggctgggata
    12001 agcgtaaagc atatgagcgt ggctatcgtg aaggctggcg cgatcgtgac gatcatcgcg
    12061 gaaaaggccg cggacatggg caccgccatt aaacgcgtcc tccaatggag cacaatgcct
    12121 gatgcgacgc tagcgcgtct tatcaggcct acaaaccggc tctttaacgc gtagggcgga
    12181 taaggcgttt acgccgcatc cgccaatagt gccagatgcg acgctcattc aactacaatc
    12241 ccagcgccgt ccccaccaac aaccagatat tcagcgccac gaccagcact acaatcaccc
    12301 agcctgtctg ttttacgcgt ttgctgttca ccagatcgcc catcaacttg ctgtcactgg
    12361 tgaaaatcag cagtggaacc agcgccaggg cgataccaaa acttaacagc acctgactca
    12421 taaccagaat ccgtgtcgga tctaatccca tcagaatgac aataaatgac ggcaacatgg
    12481 tgactgtacg acgcacccac agcgggatat ggaagcgaat gaatccctgc atcaccacct
    12541 gccccgccag tgtccccacc accgttgagg acagtccggc agcaaccaga cttaacccaa
    12601 agaccgttgc cgcagcatgg cttaacagcg gttgcagcgt cagataagcc tcatcaagat
    12661 cggcaacacc agtatgacca gaaaagtgga acgccgccgc agctgtagcc atcatcgcca
    12721 gattgacaaa accggcaatc gtcatggcga tagccacatc ccatttggtg gcggaataac
    12781 gttgttgacg cgaaccgcca tgtaaatgct gagtgagcga agagtgcaaa taaatcacat
    12841 gcggcataat cgtcgcccct aacacgcctg ctgccaggaa gaccgcctcc gaagtaggta
    12901 aactcgggat caccattcct ttacccagct gcgccaggtt aggctgggag aaaatcaact
    12961 cgacaatgta agccgcggca acaaacaaca gtaacccgcc aatcactttc tccagtggtt
    13021 tttgcccgcg acgttgcagc attaaaatca ggaaagtcgc gatccccgtc agcaccgcgc
    13081 cctgcaacaa cgagacacca agaatgagtt taaaaccgat cgccgcacca ataaattccg
    13141 ccaggtcggt tgccatcgca ataatttctg cctgaaccca atagaaccac actacgggac
    13201 gcggatagtg atcacgaatc tgctccgcca gattttttcc ggtggcaatc cctagtttgg
    13261 cagagaggat ctgaatcagc atcgccatca ggttggccca aacgacaacc cacagtagct
    13321 gatagccgaa gctggcaccc gcctgaatat tggtcgcaaa gttaccggga tcgatataac
    13381 caatcgccgc aatgaacgca ggtcccatta atgcgagcct catcttgcgc gccgcccgtc
    13441 cgctgctact ctcaacgcga tagttcgtca tcttgtgcct ctaaaacata gcctttgcta
    13501 tgtttcatgc tatgataaac gagaatgatt atcaaattca tttaaatgga ttgtggtgat
    13561 ttctctgata gaccaggatt atgactacga aaagattgat gacttgaatg ttatggttat
    13621 gtttaatgtt agcacattta cataactttc agcttccata cacaacatag cagaaatgta
    13681 tgacagatca ctatttttga agcctgtcac aggacgtcat tatagtgtgt gtcagatctc
    13741 gttttcctta accatgttac atagaatgtg cacggaaatt taacctgcct catatttgga
    13801 gcaaatatgg accgcgtcct tcattttgta ctggcacttg ccgttgttgc gattctcgca
    13861 ctgctggtaa gcagcgaccg caaaaaaatt cgtatccgtt atgttattca actgcttgtt
    13921 atcgaagtgt tactggcgtg gttcttcctg aactccgacg ttggtttagg cttcgtgaaa
    13981 ggcttctccg aaatgttcga aaaactgctc agatttgcca acgaagggac taacttcgtc
    14041 tttggtagca tgaatgatca aggcctggca ttcttcttcc tgaaagtgct gtgcccaatc
    14101 gtctttatct ctgcactgat cggtattctc cagcacattc gcgtgttgcc ggtgattatc
    14161 cgcgcaattg gtttcctgct ctccaaagtc aacggtatgg gcaaactgga atcctttaac
    14221 gccgtcagct ccctgattct gggtcagtct gaaaacttta ttgcctataa agatatcctc
    14281 ggcaaaatct cccgtaatcg tatgtacacc atggctgcca cggcaatgtc caccgtgtcg
    14341 atgtccatcg ttggtgcata catgaccatg ctggaaccga aatacgtcgt tgctgcgctg
    14401 gtactgaaca tgttcagcac ctttatcgtg ctgtcgctga tcaatcctta ccgtgttgat
    14461 gccagtgaag aaaacatcca gatgtccaac ctgcacgaag gtcagagctt cttcgaaatg
    14521 ctgggtgaat acattctggc aggtttcaaa gttgccatta tcgttgccgc gatgctgatc
    14581 ggctttatcg ccctgatcgc cgcgctgaac gcactgtttg ccaccgttac tggctggttt
    14641 ggctacagca tctccttcca gggcatcctg ggctacatct tctatccgat tgcatgggtg
    14701 atgggtgttc cttccagtga agcactgcaa gtgggcagta tcatggcgac caaactggtt
    14761 tccaacgagt tcgttgcgat gatggatctg cagaaaattg cttccacgct ctctccgcgt
    14821 gctgaaggca tcatctctgt gttcctggtt tccttcgcta acttctcttc aatcgggatt
    14881 atcgcaggtg cagttaaagg cctgaatgaa gagcaaggta acgtggtttc tcgcttcggt
    14941 ctgaaactgg tttacggctc taccctggtg agtgtgctgt ctgcgtcaat cgcagcactg
    15001 gtgctgtaaa accatacata aaaaagccgg ggatcattcc ccggcttttt tacgcctgtt
    15061 aatcagctaa tggctgcggg cgaccaatca aatacccttg cagatattgc accccgagct
    15121 tatgcaatag cgcctgctgc tgttgcgtct cgacaaactc cgcgaccaca ctcaatgact
    15181 tcgctttcgc cagatcggta attgatctca caatcatcgc atccagcgta ttcgtgacaa
    15241 tatctttcac aaagacgcca tcaattttga tgatatcagc ctgcaaacgc tttaaccgtt
    15301 cgtagttggc atatccggtg ccaaagtcat caatcgcaat ccggaaacca aacttatgca
    15361 gctgctcgat gttgtacatg ctgctttctg cgttagaaaa cgcctgctcc tcggtgatct
    15421 caagaatgac cgcctgcggg gagatgtgat aacgcttaaa cagacgaata atccgcccgg
    15481 caatattctt ttgcagcagc gtgagcggca ttaaattgac tgaaaagcgc ggtccttttt
    15541 tgtcgcaagg gtgtgttgcc agccacttca acaaggattc cagcacttgc aaatcaaaac
    15601 gcgcgctaag gttaaactga gcaataaggg gcagaaactt atccggggtc ataatgccgc
    15661 cgtcatattt cagtcgcgcg aggatctcat cataaccttc accctctttg ttgcgaattg
    15721 gctgggcgta gagcagcaaa tcaccctgat ctaacgccgt gcgaatggta ttcagcaata
    15781 gcacctgttt agtggtctgc ccggaaacca tctcctctct gctatccagc gccagcacat
    15841 gatgatgtgc gcaggattgc tccgccagcc agcttaactg ccccaacaag ggttgcaggg
    15901 tttcctgatt tccatcaaaa cgcccccagg cagcaccata gcccatatcc agcccggtat
    15961 tgttccagtg aatttgccga ctattcagga tgttaaccat atgctggagt cgcccttccg
    16021 tttccggccc gctcagcact aacagcagtt cactacccgg caattgatat aacttttcgt
    16081 tttcctgcat caacggcagc agcgtacggt aaattgatcg gatacagtga acgcgcatca
    16141 ttaagccgta atgacgactc ataaactcaa gattatcaat gcgcaggcag caaaactctt
    16201 gcccgcctct tgctccggcg cttgttccaa cgcacggaag ttgggcagga gcgttaacgg
    16261 atcggtcagc gcctgcaaat gccagcggcg attaagccat tcactgcgat gataaatgcg
    16321 caccatatag agcaggcaaa cgctaaagga aatcagcacc gcaagaataa atgccagcga
    16381 atattcggtt tccacccctt gcaaaaagtt ctggttgtaa ttcagaaggc aaagcgttga
    16441 aacagccctg gtgagattta aaaacggata gcgaagctta ccgaccccga gggtaaagat
    16501 gatgaagaaa acgggtacca ggtaaccggc aataaagtcg ttttcataag gtgtgcacaa
    16561 cagaagtaga agcacgctta acgctgccag ccagcttaag gtaaatgcgc gtttctcttt
    16621 gcccaacgac ggcgcgatat ccctgcgcca caatatctgc gcaaagtggg gacttacaat
    16681 catgcgagtg agatagtaga aaagcatgtt gtaaataagc acagcggtga aaaggcttag
    16741 caaatcaacg accgtgaaaa tggcatccgc atcgccgaaa aaggtggata tctttagcgg
    16801 gaaatcaaag aagctcccca caagatacat gctgcatttg atgccgatcg gcgtcaccaa
    16861 accaagccag aacagacgtt gccagacata tcggctggtc aatccataac gccaacgcgt
    16921 ccccagctgc cagcgtaata ttgcgcaggc acacaacacg acaaacgtct ggctaccgag
    16981 catgaccgca gtctgtaaaa aagagagatg aaagttccac agattggtgc aaaacatccc
    17041 gcataaaacg ggaacgacac cgcgccagcc aaagataaac aagattgaca gcattacgca
    17101 cagtggcatc cacgccagaa agatataact ggaatggata atggcaagtg gcgaaataaa
    17161 acgggatagc tgaataagta ccacggtgag cgtaaacgct agtgtgaata tcttgatatt
    17221 ttttatcagg ttatgctcca caaacatgag tacactactc tcactgatcg gggttttcag
    17281 gcacacaaat aataagttta ttcgcatcgc gaagcaaaca gggggccagc ctgttgccga
    17341 tttttcatcc aatgatgctt tcaccaggcg gcactaaccc ggacgtagac tgtattacaa
    17401 aagcggcaaa aagcagagac aaaaaacccc cgctttgcag cgagggttgg aaatttggtg
    17461 gagctaagcg ggatcgaacc gctgacctct tgcatgccat gcaagcgctc tcccagctga
    17521 gctatagccc cacgatgcgt ttacgtacca aatttgctgg gtgcaaaatt tggtggagct
    17581 aagcgggatc gaaccgctga cctcttgcat gccatgcaag cgctctccca gctgagctat
    17641 agccccgtca cgtaaagctt gtcgagttga cgggcggcat catatgaatt ccgcccgaat
    17701 gtgtcaacgg caaattgcaa cgtgtagttt caatcgctga aaaatcaggc aaatgaacaa
    17761 ttttggaaaa ccgctcgcat tcagtagtta ttcatgtcac ggtttcctgt aaagtggtgt
    17821 tataaaatga actactaata gacccacata cattcaggga attgttatgt tcaaggagag
    17881 gatgacacca gatgaacttg ccagactgac cggctatagc cgccagacca ttaataaatg
    17941 ggtacgcaag gagggctgga caacgtcacc aaaacctggc gtccagggtg gcaaagcccg
    18001 actggttcac gtcaatgaac aggttcgtga atatattcgc aatgctgaac gtccagaagg
    18061 tcagggagaa gcgcctgccc tttctggtga tgcgccgctt gaagtattgc tggtgacact
    18121 ggcgaaagag atgacgccag ttgaacaaaa acagtttaca tccttgcttc tgcgggaagg
    18181 gattatcgga ttgttacaac gcttagggat tcgcgatagc aaataatatg aaaagattac
    18241 gcaataaaat gaccaccgaa gaactggctg aatgcctcgg tgtggctaaa caaactgtta
    18301 accgttggat cagagaaaaa ggctggaaaa cggaaaaatt tcccggcgtg aaaggcggtc
    18361 gtgccagact tattctggtc gatacgcaag tttgcgagtt tattcagaac acgccagcct
    18421 tccataacac accaatgttg atggaagctg aagagagaat tgctgaatac gctcctggag
    18481 cacgcgcccc ggcgtatcga caaatcatca acgcgataga taatatgaca gacattgagc
    18541 agggaaaagt cgcgcaattt ttgtcacgtg aaggaattcg caatttcctc gcccgtctcg
    18601 atattgacga atcagcataa ataaaaaacg gcaggatatt atctcctgcc gtttatcttt
    18661 ttacacgcta attactgctg attttcgcgt tcagcaataa aatccagcgc tttgttgata
    18721 cgctcgatac tgcgggtctt accaattgcg tgaacggtaa catccagtgc tggagactgc
    18781 cccgccccgg ttacggcgac acgcagcggc ataccaactt tacccatacc cacttccagc
    18841 tcatccgccg tcgcctgaat agcgtgatga acgttttcag cggtccagtc agtaatcgcg
    18901 gccagtttgt cacgaaccac ttccagcggc tgacgcgcta ccggacgcag atgttttttc
    18961 gccgcatcgg catcgaactc agcaaaatct tcgtagaaat aacggcagct ctgtgccatc
    19021 tctttcagcg tcttgcagcg ttcgcccagc agtttcacca gatcagccag ctgcgggccg
    19081 ttacgggtat cgatattttc ctgctcaatg tgccactgta agtgagtagc aacatactcc
    19141 ggcggcagcg cgttaatgta gtgatggttc agccacagca gcttgtcggt gttgaacgca
    19201 ctggcagatt tgctgacggc attcaaagtg aagtatttga tcatctcttc acgagtgaag
    19261 atttcctgat cgccgtggga ccagcccaga cgcaccagat agttcagcag tgcttctggc
    19321 aaataaccgt catcacgata ctgcattacg ctgactgccc cgtgacgttt ggacagtttt
    19381 ttaccgtcat cgccattgat catagaaacg tgcgcgtaaa ccggcaccgg cgctttcagg
    19441 gccttaagaa tgttgatctg gcgcggcgtg ttgttgatat ggtcttcgcc acggataacg
    19501 tgggtgattt ccatatccca gtcatcgaca accacacaga agttataggt tggggaacca
    19561 tcggtacggc ggataataag atcgtccagt tcctggttgc tgaactcgat cggaccacgg
    19621 atctgatcgt caaaaacaac agaaccttcc tgcgggttag caaaacgtac aacacacggt
    19681 tcatcatcag catgatgctc atggctgtgg cggcagcgac cgtcataacg cggcttctca
    19741 cctttcgcca tttgctcttc gcgcagcgct tccaggcgct ctttagagca atagcattta
    19801 taagcagtgc cctcttccag catctgatcg atcaccgcgt tgtagcgatc aaaacgtttg
    19861 gtctggtagt acggaccttc atcccactcc aggctcagcc agttcatgcc atccataatg
    19921 gcttcgatag cttccggcgt ggaacgctca agatcggtgt cttcaatacg cagcacgaac
    19981 tcaccgccgt ggttacgtgc aaaaagccag gagtaaagag cagtacgcgc gccgccaacg
    20041 tgcagatagc ctgttgggct tggcgcgaag cgagttttga ttttcatgaa atggccttac
    20101 gtttagaaag atgccgacaa ccggcaaatc ctggaaaaat tgagtgggcg atattctatc
    20161 actcccgcct gattcctcaa tgtagttcgg gtttctacct tacgcattat tgttttcgtt
    20221 gacaaattgc gcaacacggt ctgttttgcg atcatcttgc ttaaatttac gacgaacgaa
    20281 caatttcttt agaaaatgcg ttgactcatt ttgaactctc cctataatgc ggctccacac
    20341 agcgggggtg attagctcag ctgggagagc acctccctta caaggagggg gtcggcggtt
    20401 cgatcccgtc atcacccacc aacatacttt atgtagtctc cgccgtgtag caagaaattg
    20461 agaagtgggt gattagctca gctgggagag cacctccctt acaaggaggg ggtcggcggt
    20521 tcgatcccgt catcacccac caatttctcg ccagctaaat ttcttgtaaa aatgtgaagt
    20581 accgaagtgg gtgattagct cagctgggag agcacctccc ttacaaggag ggggtcggcg
    20641 gttcgatccc gtcatcaccc accacttcgg gtcgttagct cagttggtag agcagttgac
    20701 ttttaatcaa ttggtcgcag gttcgaatcc tgcacgaccc accaatgtaa aaaagcgccc
    20761 taaaggcgct tttttgctat ctgcgatact caaagattcg aacctgcagc aggtttgagt
    20821 tgagcgcagc gaaacaacgg agccgctcgc ggcgacggcc cgaagggcga gcgaagcgag
    20881 tcatcctgca cgacccacca atgtaaaaaa gcgccctaaa ggcgcttttt tgctatctgc
    20941 gatttgtaag attgattttt cttctcaagt tccttgttct tctgccgata ttacctcgta
    21001 cagtgacgag gactgaacaa tggccactat ctcttcaact tcaattcctt ccatacaaac
    21061 acagagtagt aacagggcat cacaaggtag cgatgttgct tcaaaaatcg cgcggataag
    21121 tcagcaaatc atcaaactca ctcaacaaat taaagagatt gtagacacta gcggcagtgc
    21181 tgaagacaaa caaaaacagg ccgaattgat tcaacagcaa atcaccctac tggaaacaca
    21241 actggcgcag ttgcagagac agcaggcaga aaaggcgcag gagaaagagc agcgtttgtc
    21301 tctgaatgta tcccttctaa atcctgttga aaatacgact catatcgata tctatatctg
    21361 atacaacgga gccccttccc gaccagccat tagcgcattg ccctggtctc gtaccacgtc
    21421 ccataacgct tcagcggcgg tggaaagtga acggttcttc ctgcgcacca gcatcaactg
    21481 gcgttcaaca accggcgtaa tgcgctttac caccagtggg ctaccttcag gcaatggtag
    21541 cgccagtgcg ggaagaatac tgatgccgat gcctgcggct accatcggga acagcgtcgc
    21601 cggatgtcca atctcctgca caatattcgc ctgaatacca ttgcgcgcca gcgctgcgtc
    21661 aatcagcggt cggctgcctg acgcgtaatc ctgcaacacc aatttcgctc cctgtaatgc
    21721 ctgccagggt acataatctt ccacggccaa agcgctatcg cgatggcaaa gcagaaagaa
    21781 aggctcggaa agaatcgctt cacattgcag atcgcccact gggcctggat cgatgacgat
    21841 gcaaaaatcg acatctccct ggcgaatact ttccatcacc cactgctgcg gacgatcgtg
    21901 caggacaaac tgaatatctg gatagcggcg atggctttca gcaatgcatt gcggaataag
    21961 atgcgcggaa atggtctggc tggcagcgac ccgaactttt ccgctcagtt gttgccccat
    22021 acgcccggta tcgcgcaacg tgctgttaag ttcatccagc agtcgctcaa gacgcaaagc
    22081 caactgctgc cctgcatctg taagcaccac ttcacgcgtg gttctgtcca gcaggcgaac
    22141 accagtatga ttttccagct ccttcacact gtgacttact gccgactggc tcaagccgat
    22201 acgctctcct gcacgactaa aacttttctc ctgcgctact gtgacgaaaa cctttaattg
    22261 ttttaaagaa taattcatct gttttcttca tgaatggatg caataaatca attttatttc
    22321 tcaaactgga agaagcacaa tagaaccatc gatcatctgg agtctttatg aaactttttc
    22381 gtatcctcga tcctttcacc ttaaccctga tcacggtggt gttgctggcc tctttctttc
    22441 cggccagagg cgatttcgtc cccttctttg aaaatctgac caccgcagct attgccctgc
    22501 tgttctttat gcacggcgcg aagttgtcgc gtgaggcgat tattgctggc ggtggtcact
    22561 ggcgactgca tttgtgggta atgtgcagca ccttcgtgct gtttccgatt ctgggtgtac
    22621 tgtttgcctg gtggaaaccg gtaaatgtcg acccgatgct ctactccggt tttctctact
    22681 tgtgcattct cccggctacc gtgcagtctg caatcgcctt cacgtcaatg gcgggcggta
    22741 acgtcgcggc ggcggtttgt tctgcgtcag catccagcct gctggggatt ttcctttcac
    22801 cattgctagt tggtctggtg atgaatgttc acggtgcagg gggcagcctt gagcaggtcg
    22861 gtaaaattat gctgcaactg ctgctgccgt ttgtgttggg gcatctttcc cggccgtgga
    22921 ttggtgactg ggtgtcgcgc aataaaaaat ggattgcgaa aactgaccag acgtccattc
    22981 tgttggtggt ttatacagcg ttcagcgaag ccgtcgttaa tggtatctgg cataaagttg
    23041 gctggggatc attgctgttt atcgtggtgg tcagctgcgt tcttctggct atcgtgattg
    23101 tagttaacgt ctttatggca cgccgactgg gcttcaataa ggcagatgaa attactatcg
    23161 tcttttgtgg ttcgaaaaag agtctggcaa atggcatccc gatggcaaac attctgttcc
    23221 ccacatcggt gatcggtatg atggtgctgc ccctgatgat tttccatcag atccaattga
    23281 tggtctgtgc ggtgctggcg cgtcgataca aacgccagac cgaacagtta caggcgcagc
    23341 aggaaagcag cgccgataaa gcttaaagcg gacgcttcag gggctggacc agttgcgtca
    23401 gcccctcggt tttaatcagc agcgtgattt gcattagctc accgagcttc cctgccggga
    23461 actcatcttt gcgggcaaac cacagcagat actcctccgg caggtcgatt aagcgacgcc
    23521 ctttgtattt gccaaacggc attatcgtat tggctatttc aatcagctgc tctttttcca
    23581 tctcagctac ccagcaaacg cagcatttcc gcttcgtcga tgacttcaat gcccagttcc
    23641 tgcgccttcg ccagtttaga tcctgcagct tcacccgcta tcaccagatc ggttttcttc
    23701 gacacgctgc ccgcgacttt cgcgcccagt tcgaccagtc gagctttagc gtcatcacgc
    23761 gacatctggc ttaagctgcc cgtaagcacc acggttttac cagcaaacgg gctgtcaatc
    23821 tcttccgcgt tgataacgat cggcgcaggc cagtgaacac cttccgccaa cagctcgctg
    23881 atgacattgc ggttgctttc ttcggcaaag aagttgtgaa cgtgggatgc aacgacaatg
    23941 ccaacatcag gcaccttttg cagctcttca atcgaagcgg cttccagcac ttccagcgtg
    24001 ccgaaatatg ccgccagacc tgctgcggtg gcctcgccga cttcacggat gccaagtgca
    24061 tagaggaagc gagcaaaggt ggtttctttc gctttttcca gcgcgttaac cacgttttgt
    24121 gccgattttg gccccatacg ctccagtccg gtcagtttgc ctgcggtgag tttgaacaga
    24181 tctgccggag tgtggacata ttctttttca accagctgat cgatgatttt gtcgcccatt
    24241 ccgtcaacat ccatcgcacg gcgggaaaca aagtgtttca gcgactcttt acgctgcgca
    24301 ccgcaaatca ggccaccggt acagcgggca accgcttcac cttccacacg ctcaacgtca
    24361 gaaccacata ccggacaatg cgtcgggaat acaacctcac gggtatcttc cgggcgttca
    24421 gaaagcacga cgttaaccac ctgcgggatc acgtcgccag cgcggcgaat caccacttta
    24481 tcgccaatgc gtaaaccaag acgttcgatt tcatccgcat tgtgtaaggt tgcgttactc
    24541 accagcacgc cagcaacatg gacaggttcc agacgcgcaa caggcgtaat cgcgccagta
    24601 cgcccaacct gaaactcgac gtcacgcaca aaggtcatct gctcctgcgc cgggaattta
    24661 aacgctaccg cccagcgcgg ggcacgcgcg acaaagccaa gctgctcctg ctgttccagt
    24721 gagttgacct taatcaccac gccgtcgata tcaaagccca gcgtcgggcg gtcttcttcc
    24781 actttgtgat agaacgccag cacttcttcc gccgattcac aaagcgttac ccgatcgctg
    24841 accggcaacc cccacttttt aaattgcagt aaacggccaa gatgagtatc cggcagctcg
    24901 ccaccttcca gaacaccaac gccatagcag aaaaaagtga gcggtcgctt cgctgtaata
    24961 cgcggatcaa gctgacgcag tgaaccagct gccgcattac gtgggttagc aaaaactttc
    25021 ccgcccgtgc gtcgcgcatc ttcgttaatc ttttcgaacc ccgcctgcgg caggaacact
    25081 tcaccacgca cttccagacg cgccgggata ttctctccgt gcagcttcag cggaatggcg
    25141 cgaatagtac gcacattaga cgtgatatct tccccggtgg tgccatcgcc acgggtcgcg
    25201 gcactgacta aaacgccatt ttcatacaga atactgacgg caaggccatc cagcttcagc
    25261 tcacagcacc aggtgacttt ctcgttgttt ttcagacggt cctgcacacg tttgttgaaa
    25321 gcaagaaagc tttcttcatc aaaaacgtta tccagtgaca gcattggtac ttcatggcgt
    25381 atctggctga aagccgccag cggcgcagca cctacacgtt gggtaggcga atcaggcgta
    25441 atcagttctg gatgtttggt ttccagctcg cgcagttcgc gcatcagcct gtcgtattca
    25501 gcgtcgggaa tttccggcgc atccatcaca tgataaagat attcatgatg gcgaagcgtc
    25561 gttcgcagtt ctgtcagttg ttgttcgatt gattccatat cgcaccatca atgctaaaaa
    25621 cccccgacaa gcgggggttc gaagaggagt taatttgcct taagtgtatc aggcgttggc
    25681 gtctttgact tcgcggatga tgtcctggta ctcgcgcaat ttctgcggag tcatcatacg
    25741 gcgctgatcg tcaagcacga caccgcccac ttcatcggca atatgctgcg cagattgcag
    25801 catcagcttg aagttctgca gctcgtcacc gtaagacggt acctgcataa agatagtgac
    25861 acccggagta gtgaaatcct tcatttcagg atcaaaggtt cccggtttca ccatattcgc
    25921 caggctgaat aacgccgggc cgctgccatc cgggctaaga tgacgatggt aaatattcat
    25981 atcgccaaaa atgaagcccg cttgttgaat gctgttaaga agcagttcac cgtttagctc
    26041 gctaccgtga tgcgccgcga cgttcatgat aatcaccgct tctttgcgct tcggtttatc
    26101 cataactgga gccggttccg ctacaggctc aggctgtggt gccgctacgg gttctgcagg
    26161 ctggaaagcc tgttgtgccg gttgcggtgc tgaatgcaca ggctgcggcg ctggcgcgac
    26221 ctgtggcgaa actggctgct gcaacggctg ttcaggctgc ggctgatagg caggctgctg
    26281 catcggctgc gctggacgcg gagcatgttg cggcggtacc tgcgcttcag gcggctgctg
    26341 gatcggttga cgcggctgcg cagacgcata aggcggttgg tactggtgtt gcggcgacgg
    26401 acgagcagcc tcatgctcct gagcgttagc cggggcatga ttcacgcggt gaacacgaac
    26461 ctcaccaacg ccctcatcat cttcgacatc ctcgtcataa gaatcgtcgt cacgttttga
    26521 cttcattcgt tttaatggcc gatcgcggaa catagaagat cgttctttac ggctggtcca
    26581 gaaaccatgt accagtaaag cgattatggc gatcgcgcca acaatgatta atatcagacg
    26641 caaatcctgc atcattatat tctctgttgt tctaacacct tgccaccacg gcaaacattt
    26701 actcactaag agtatttgcc gattacctca agtgcaagtg cactattaac tttcacagca
    26761 caaagataga tgaaatcgtg ctttttgctg ttttttcgaa catatcctaa ctgtccattg
    26821 cgcaattacc cggtaaaata cgcagaattt tcctgggatt ggtcaaaagg agctcatcct
    26881 gattatggtt tcatcattca catctgcccc acgcagcggt ttttactatt ttgcgcaagg
    26941 ctggaagctc gtctcgcaac ctgggattcg gcgtttcgtt attttaccgc tgctggtcaa
    27001 tattttgttg atggggggcg cattctggtg gctctttaca cagctcgatg tctggatccc
    27061 gactctcatg agttacgttc cggactggct acaatggctg agttatctgt tgtggcctct
    27121 ggctgtcatc tctgtgctgt tagtgtttgg ctatttcttc tccacgattg ctaactggat
    27181 tgccgctccg tttaacggtt tattggctga acaactggaa gcacgattga ctggcgctac
    27241 accgccagat accgggattt tcggtatcat gaaagatgtg ccgcgaatca tgaaacgcga
    27301 atggcaaaaa tttgcctggt atctgccgcg cgcaattgta ttactaattc tttacttcat
    27361 ccctggtatt gggcaaaccg tcgcgccggt actgtggttc ctgtttagcg cctggatgtt
    27421 agccatccag tattgcgatt accccttcga taaccacaaa gtgccgttta aagagatgcg
    27481 caccgccctg cgcacacgca aaatcaccaa tatgcagttt ggtgctttaa ccagcctgtt
    27541 tacgatgatc ccgctgctta atctgttcat catgcccgtt gccgtttgtg gcgcgacggc
    27601 gatgtgggtc gattgctatc gcgataaaca cgcgatgtgg cggtaacaat ctaccggtta
    27661 ttttgtaaac cgtttgtgtg aaacaggggt ggcttatgcc gccccttatt ccatcttgca
    27721 tgtcattatt tcccttctgt atatagatat gctaaatcct tacttccgca tattctctga
    27781 gcgggtatgc tacctgttgt atcccaattt catacagtta aggacaggcc atgagtaaga
    27841 tttttgaaga taactcgctg actatcggtc acacgccgct ggttcgcctg aatcgcatcg
    27901 gtaacggacg cattctggcg aaggtggaat ctcgtaaccc cagcttcagc gttaagtgcc
    27961 gtatcggtgc caacatgatt tgggatgccg aaaagcgcgg cgtgctgaaa ccaggcgttg
    28021 aactggttga accgaccagc ggtaataccg ggattgcact ggcctatgta gctgccgctc
    28081 gcggttacaa actcaccctg accatgccag aaaccatgag tattgaacgc cgcaagctgc
    28141 tgaaagcgtt aggtgcaaac ctggtgctga cggaaggtgc taaaggcatg aaaggcgcaa
    28201 tccaaaaagc agaagaaatt gtcgccagca atccagagaa atacctgctg ctgcaacaat
    28261 tcagcaatcc ggcaaaccct gaaattcacg aaaaaaccac cggtccggag atctgggaag
    28321 ataccgacgg tcaggttgat gtatttattg ctggcgttgg gactggcggt acgctgactg
    28381 gcgtcagccg ctacattaaa ggcaccaaag gcaagaccga tcttatctct gtcgccgttg
    28441 agccaaccga ttctccagtt atcgcccagg cgctggcagg tgaagagatt aaacctggcc
    28501 cgcataaaat tcagggtatt ggcgctggtt ttatcccggc taacctcgat ctcaagctgg
    28561 tcgataaagt cattggcatc accaatgaag aagccatttc taccgcccgt cgtctaatgg
    28621 aagaagaagg tattcttgca ggtatctctt ctggagcagc tgttgccgcg gcgttgaaac
    28681 tacaagaaga tgaaagcttt accaataaga atattgtggt tattctacca tcatcgggtg
    28741 agcgttattt aagcaccgca ttgtttgccg atctcttcac tgagaaagaa ttgcaacagt
    28801 aatgccagct tgttaaaaat gcgtaaaaaa gcaccttttt aggtgctttt ttgtggcctg
    28861 cttcaaactt tcgcccctcc tggcattgat tcagcctgtc ggaactggta tttaaccaga
    28921 ctaattattt tgatgcgcga aattaatcgt tacaggaaaa gccaaagctg aatcgatttt
    28981 atgatttggt tcaattcttc ctttaacggc ataatgttta atgacgtacg aaacgtcagc
    29041 ggtcaacacc cgccagcaat ggactgtatt gcgctcttcg tgcgtcgcgt ctgttaaaaa
    29101 ctggcgctaa caatacaggc taaagtcgaa ccgccaggct agactttagt tccacaacac
    29161 taaacctata agttggggaa atacaatgtt ccagcaagaa gttaccatta ccgctccgaa
    29221 cggtctgcac acccgccctg ctgcccagtt tgtaaaagaa gctaagggct tcacttctga
    29281 aattactgtg acttccaacg gcaaaagcgc cagcgcgaaa agcctgttta aactgcagac
    29341 tctgggcctg actcaaggta ccgttgtgac tatctccgca gaaggcgaag acgagcagaa
    29401 agcggttgaa catctggtta aactgatggc ggaactcgag taatttcccg ggttctttta
    29461 aaaatcagtc acaagtaagg tagggttatg atttcaggca ttttagcatc cccgggtatc
    29521 gctttcggta aagctctgct tctgaaagaa gacgaaattg tcattgaccg gaaaaaaatt
    29581 tctgccgacc aggttgatca ggaagttgaa cgttttctga gcggtcgtgc caaggcatca
    29641 gcccagctgg aaacgatcaa aacgaaagct ggtgaaacgt tcggtgaaga aaaagaagcc
    29701 atctttgaag ggcatattat gctgctcgaa gatgaggagc tggagcagga aatcatagcc
    29761 ctgattaaag ataaacacat gacagctgac gcagctgctc atgaagttat cgaaggtcag
    29821 gcttctgccc tggaagagct ggatgatgaa tacctgaaag aacgtgcggc tgacgtacgt
    29881 gatatcggta agcgcctgct gcgcaacatc ctgggcctga agattatcga cctgagcgcc
    29941 attcaggatg aagtcattct ggttgccgct gacctgacgc cgtccgaaac cgcacagctg
    30001 aacctgaaga aggtgctggg tttcatcacc gacgcgggtg gccgtacttc ccacacctct
    30061 atcatggcgc gttctctgga actgcctgct atcgtgggta ccggtagcgt cacctctcag
    30121 gtgaaaaatg acgactatct gattctggat gccgtaaata atcaggttta cgtcaatcca
    30181 accaacgaag ttattgataa aatgcgcgct gttcaggagc aagtggcttc tgaaaaagca
    30241 gagcttgcta aactgaaaga tctgccagct attacgctgg acggtcacca ggtagaagta
    30301 tgcgctaaca ttggtacggt tcgtgacgtt gaaggtgcag agcgtaacgg cgctgaaggc
    30361 gttggtctgt atcgtactga gttcctgttc atggaccgcg acgcgctgcc cactgaagaa
    30421 gaacagtttg ctgcttacaa agcagtggct gaagcgtgtg gctcgcaggc ggttatcgtt
    30481 cgtaccatgg acatcggcgg cgacaaagag ctgccataca tgaacttccc gaaagaagag
    30541 aacccgttcc tcggctggcg cgctatccgt atcgcgatgg atcgtaaaga gatcctgcgc
    30601 gatcagctcc gcgctatcct gcgtgcctcg gctttcggta aattgcgcat tatgttcccg
    30661 atgatcatct ctgttgaaga agtgcgtgca ctgcgcaaag agatcgaaat ctacaaacag
    30721 gaactgcgcg acgaaggtaa agcgtttgac gagtcaattg aaatcggcgt aatggtggaa
    30781 acaccggctg ccgcaacaat tgcacgtcat ttagccaaag aagttgattt ctttagtatc
    30841 ggcaccaatg atttaacgca gtacactctg gcagttgacc gtggtaatga tatgatttca
    30901 cacctttacc agccaatgtc accgtccgtg ctgaacttga tcaagcaagt tattgatgct
    30961 tctcatgctg aaggcaaatg gactggcatg tgtggtgagc ttgctggcga tgaacgtgct
    31021 acacttctgt tgctggggat gggtctggac gaattctcta tgagcgccat ttctatcccg
    31081 cgcattaaga agattatccg taacacgaac ttcgaagatg cgaaggtgtt agcagagcag
    31141 gctcttgctc aaccgacaac ggacgagtta atgacgctgg ttaacaagtt cattgaagaa
    31201 aaaacaatct gctaatccac gagatgcggc ccaatttact gcttaggaga agatcatggg
    31261 tttgttcgat aaactgaaat ctctggtttc cgacgacaag aaggataccg gaactattga
    31321 gatcattgct ccgctctctg gcgagatcgt caatatcgaa gacgtgccgg atgtcgtttt
    31381 tgcggaaaaa atcgttggtg atggtattgc tatcaaacca acgggtaaca aaatggtcgc
    31441 gcctgtagac ggtaccattg gtaaaatctt tgaaaccaat cacgcgttct ctatcgaatc
    31501 tgatagcggc gttgaactgt tcgtccactt cggtatcgac accgttgaac tgaaaggcga
    31561 aggcttcaag cgtattgctg aagaaggtca gcgcgtgaaa gttggcgata ctgtcattga
    31621 atttgatctg ccgctgctgg aagagaaagc caagtctacc ctgactccgg ttgttatctc
    31681 caacatggac gaaatcaaag aactgatcaa actgtccggt agcgtaaccg tgggtgaaac
    31741 cccggttatc cgcatcaaga agtaattctt gccgcagtga aaaatggcgc cca
//
LOCUS       DADMKF010000034        31085 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.035, whole genome shotgun sequence.
ACCESSION   DADMKF010000034 DADMKF010000000
VERSION     DADMKF010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31085
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.035"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            <1..278
                     /locus_tag="JNC24_001430"
     CDS             <1..278
                     /locus_tag="JNC24_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005153149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HAY9158210.1"
                     /translation="SVMCDGLLVGNAEIIPFSPRRYLYHAYLAYMRAHGFGKPVTLTR
                     FGKDMPGAMAEYGREYMKRKTKHGLRSNVTLTEDSEDWMPSCAIGHK"
     gene            582..827
                     /locus_tag="JNC24_001431"
     CDS             582..827
                     /locus_tag="JNC24_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9158211.1"
                     /translation="MTTKHAKKPQSHALDLTEHWLRVAIKIIDRNAGEGYAKAHPELI
                     SAFMTTTAANFATLTEREIAEAEQVTTINVKTGEQTA"
     gene            824..1246
                     /locus_tag="JNC24_001432"
     CDS             824..1246
                     /locus_tag="JNC24_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001758993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAY9158212.1"
                     /translation="MTAQIAAYGRLVADPQLKTTSKGTQMTMASMAVPLPCSQADDGT
                     ATIWLSVLAFGRQADALAKHQKGELVSVAGNMQVSQWTGQNGETRQGWQVIADSVISA
                     RTARPGGKKGQQGQATDALNRAKQQAGNDDPYGDNIPF"
     gene            1906..3773
                     /locus_tag="JNC24_001433"
                     /pseudo
     CDS             1906..3773
                     /locus_tag="JNC24_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000860399.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
     gene            4032..4313
                     /locus_tag="JNC24_001434"
     CDS             4032..4313
                     /locus_tag="JNC24_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9158213.1"
                     /translation="MTEAEILGLIRRVAGISQQVDEQAMQPDSVTAENYARVVDEVMR
                     RDGIELNGVDMRNIRTRVLELLAYRRRSQQRRESAKNTYQWRKPEHLRR"
     gene            4953..5273
                     /gene="clpS"
                     /locus_tag="JNC24_001435"
     CDS             4953..5273
                     /gene="clpS"
                     /locus_tag="JNC24_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HAY9158214.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            5304..7580
                     /gene="clpA"
                     /locus_tag="JNC24_001436"
     CDS             5304..7580
                     /gene="clpA"
                     /locus_tag="JNC24_001436"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HAY9158215.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(7986..8073)
                     /locus_tag="JNC24_001437"
     tRNA            complement(7986..8073)
                     /locus_tag="JNC24_001437"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8037..8039),aa:Ser,seq:gga)
     gene            complement(8327..8545)
                     /gene="infA"
                     /locus_tag="JNC24_001438"
     CDS             complement(8327..8545)
                     /gene="infA"
                     /locus_tag="JNC24_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HAY9158216.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(8830..9534)
                     /gene="aat"
                     /locus_tag="JNC24_001439"
     CDS             complement(8830..9534)
                     /gene="aat"
                     /locus_tag="JNC24_001439"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HAY9158217.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGAPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWHEDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFISHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQK"
     gene            complement(9576..11297)
                     /gene="cydC"
                     /locus_tag="JNC24_001440"
     CDS             complement(9576..11297)
                     /gene="cydC"
                     /locus_tag="JNC24_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HAY9158218.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGGAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTVGLSFLDF
                     TLAFTLGGIMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRITDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALSEIL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHDAPLVLLDEPTEGLDAT
                     TESQILELLAEMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(11298..13064)
                     /gene="cydD"
                     /locus_tag="JNC24_001441"
     CDS             complement(11298..13064)
                     /gene="cydD"
                     /locus_tag="JNC24_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HAY9158219.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWAVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEVELASTDPVTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAATLRDNVLLARPDA
                     SEHELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGQIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(13187..14152)
                     /gene="trxB"
                     /locus_tag="JNC24_001442"
     CDS             complement(13187..14152)
                     /gene="trxB"
                     /locus_tag="JNC24_001442"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HAY9158220.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMERMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            14697..15191
                     /gene="lrp"
                     /locus_tag="JNC24_001443"
     CDS             14697..15191
                     /gene="lrp"
                     /locus_tag="JNC24_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HAY9158221.1"
                     /translation="MVDSKKRLGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            15326..19393
                     /gene="ftsK"
                     /locus_tag="JNC24_001444"
     CDS             15326..19393
                     /gene="ftsK"
                     /locus_tag="JNC24_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAY9158222.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTAAQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQPQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPVQQPYYAPAAEQPVQQPYYAPAPEQPVAGNAWQAE
                     EQQSTFAPQSTYQTEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPVVEETKPARPPLYY
                     FEEVEEKRAREREQLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVPPVETAAAVSPLAS
                     GVKKATLATGAAATVAAPVFSLANSGGPRPQVKEGIGPQLPRPKRIRVPTRRELASYG
                     IKLPSQRAAEEKAREAQRNQYDSGDQYNDDEIDAMQQDELARQFAQTQQQRYGEQYQH
                     DVPVNAEDADAAAEAELARQFAQTQQQRYSGEQPAGANPFSLDDFEFSPMKALLDDGP
                     HEPLFTPIVEPVQQPQQPVAPQQQYQQPQQPVAPQQQYQQPQQPVAPQPQYQQPQQPV
                     APQQQYQQPQQPVAPQPQYQQPQQPVAPQQQYQQPQQPVAPQPQDTLLHPLLMRNGDS
                     RPLHKPTTPLPSLDLLTPPPSEVEPVDTFALEQMARLVEARLADFRIKADVVNYSPGP
                     VITRFELNLAPGVKAARISNLSRDLARSLSTVAVRVVEVIPGKPYVGLELPNKKRQTV
                     YLREVLDNAKFRDNPSPLTVVLGKDIAGEPVVADLAKMPHLLVAGTTGSGKSVGVNAM
                     ILSMLYKAQPEDVRFIMIDPKMLELSVYEGIPHLLTEVVTDMKDAANALRWCVNEMER
                     RYKLMSALGVRNLAGYNEKIAEADRMMRPIPDPYWKPGDSMDAQHPVLKKEPYIVVLV
                     DEFADLMMTVGKKVEELIARLAQKARAAGIHLVLATQRPSVDVITGLIKANIPTRIAF
                     TVSSKIDSRTILDQAGAESLLGMGDMLYSGPNSTLPVRVHGAFVRDQEVHAVVQDWKA
                     RGRPQYVDGITSDSESEGGAGGFDGAEELDPLFDQAVQFVTEKRKASISGVQRQFRIG
                     YNRAARIIEQMEAQGIVSEQGHNGNREVLAPPPFD"
     gene            19548..20159
                     /gene="lolA"
                     /locus_tag="JNC24_001445"
     CDS             19548..20159
                     /gene="lolA"
                     /locus_tag="JNC24_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HAY9158223.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            20170..21513
                     /locus_tag="JNC24_001446"
     CDS             20170..21513
                     /locus_tag="JNC24_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HAY9158224.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLGTEDIEQVLTQAMEDKTRGYGGQDIVLPDETRRAIAELVNGDARRALNTLEMM
                     ADMAEVNDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            21604..22896
                     /gene="serS"
                     /locus_tag="JNC24_001447"
     CDS             21604..22896
                     /gene="serS"
                     /locus_tag="JNC24_001447"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HAY9158225.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            23135..25579
                     /gene="dmsA"
                     /locus_tag="JNC24_001448"
     CDS             23135..25579
                     /gene="dmsA"
                     /locus_tag="JNC24_001448"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
                     /protein_id="HAY9158226.1"
                     /translation="MKTKIPDAVLAAEVSRRGLVKTTAIGGLAMASSALTLPFSRIAH
                     AVDSAIPTKSDEKVIWSACTVNCGSRCPLRMHVVDGEIKYVETDNTGDDNYDGLHQVR
                     ACLRGRSMRRRVYNPDRLKYPMKRVGARGEGKFERISWEEAYDIIATNMQRLIKEYGN
                     ESIYLNYGTGTLGGTMTRSWPPGNTLVARLMNCCGGYLNHYGDYSSAQIAEGLNYTYG
                     GWADGNSPSDIENSKLVVLFGNNPGETRMSGGGVTYYLEQARQKSNARMIIIDPRYTD
                     TGAGREDEWIPIRPGTDAALVNGLAYVMITENLVDQAFLDKYCVGYDEKTLPASAPKN
                     GHYKAYILGEGPDGVAKTPEWASQITGVPADKIIKLAREIGSTKPAFISQGWGPQRHA
                     NGEIATRAISMLAILTGNVGINGGNSGAREGSYSLPFVRMPTLENPIQTSISMFMWTD
                     AIERGPEMTALRDGVRGKDKLDVPIKMIWNYAGNCLVNQHSEINRTHEILQDDKKCEL
                     IVVIDCHMTSSAKYADILLPDCTASEQMDFALDASCGNMSYVIFNDQVIKPRFECKTI
                     YEMTSELAKRLGVEQQFTEGRTQEEWMRHLYAQSREAIPELPTFEEFRKQGIFKKRDP
                     QGHHVAYKAFREDPQANPLTTPSGKIEIYSQALADIAATWELPEGDVIDPLPIYTPGF
                     ESYQDPLNKQYPLQLTGFHYKSRVHSTYGNVDVLKAACRQEMWINPLDAQKRGINNGD
                     KVRIFNDRGEVHIEAKVTPRMMPGVVALGEGAWYDPDAKRVDKGGCINVLTTQRPSPL
                     AKGNPSHTNLVQVEKV"
     gene            25590..26207
                     /gene="dmsB"
                     /locus_tag="JNC24_001449"
     CDS             25590..26207
                     /gene="dmsB"
                     /locus_tag="JNC24_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HAY9158227.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            26209..27072
                     /locus_tag="JNC24_001450"
     CDS             26209..27072
                     /locus_tag="JNC24_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HAY9158228.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLVVTMVLGVVFVWMMVRVYNSIDTVPTWYSIWTPMGFFLTMF
                     MGGPLLGYLLLSLAGVDSWAMRLLPAISVLALVVSGVVSVMQGAELATIHSSVQQAAA
                     LVPDYGALMSWRIVLLAVALCLWIAPQLKGYQPAVPLLSVSFILLLAGELIGRGVFYG
                     LHMTVGMAVAS"
     gene            complement(27108..27734)
                     /locus_tag="JNC24_001451"
     CDS             complement(27108..27734)
                     /locus_tag="JNC24_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAY9158229.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVPELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            28049..29197
                     /locus_tag="JNC24_001452"
     CDS             28049..29197
                     /locus_tag="JNC24_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY9158230.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLASFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITAMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFTAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
     gene            29407..30837
                     /locus_tag="JNC24_001453"
                     /pseudo
     CDS             29407..30837
                     /locus_tag="JNC24_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415419.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
     gene            complement(30838..>31085)
                     /locus_tag="JNC24_001454"
     CDS             complement(30838..>31085)
                     /locus_tag="JNC24_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY9158231.1"
                     /translation="DVDAELDAVLMGAGIGYLLYEQIKEYLDTGRLECVLEDWITERP
                     GFQIYYPNRQYMSCGLRAFLDYVKTGQICQSQRHRPQ"
BASE COUNT         7870 a         7502 c         8287 g         7426 t
ORIGIN      
        1 cgtcggtaat gtgtgatggt ctgttagtgg gtaatgccga aattattccg ttcagtccgc
       61 gcaggtatct ttatcacgcc tatctggcat atatgagggc acacggattc ggtaaacctg
      121 taacactgac gcgcttcggt aaagatatgc cgggggcaat ggcggaatat ggcagggagt
      181 atatgaaacg gaaaacgaag cacggtttgc gttcaaatgt gaccctgaca gaggattcag
      241 aagactggat gccatcatgt gcaataggtc acaaatgacg atggaaaaaa ttaaacttat
      301 ggaataactg ttcaccactg ttcaccctgc aataaatgtc ttttatatca gtatattata
      361 gggtgaacag ttatttatga actgttcacc aaactattca ctgttcacct ttttgattct
      421 ttattgagct ttaagggtga acagtggtga acagttggtg aatagttttt gtgaaactgt
      481 taacccatta acatcatgaa taaaaaggga aaattgcaaa aggtgaacag gtgaagggtt
      541 aaaatgcaaa aattttattt tattgctgtg aggtaaagcc tgtgacaacg aagcacgcca
      601 aaaaaccaca atctcacgcc cttgatttga cagaacactg gctgagggtg gcgataaaaa
      661 tcatcgaccg caacgccggg gaaggatacg cgaaagcaca tcccgaactg ataagcgcat
      721 tcatgaccac gacggcggca aactttgcca cgctgacaga acgggagatt gccgaagcgg
      781 aacaggtgac aaccatcaac gttaaaaccg gagagcagac agcatgacag cacagatagc
      841 ggcttacggg cggctggtgg ctgacccgca gttaaagacc accagcaagg gtacgcaaat
      901 gacgatggcg agtatggcgg ttcccctgcc gtgcagccag gcagatgacg gaacggcgac
      961 gatatggtta tccgtcctgg cgtttggcag acaggctgac gcactggcaa agcaccagaa
     1021 aggcgagctg gtgagcgtgg cgggtaacat gcaggtaagc cagtggacag gccagaacgg
     1081 agaaacgcgg cagggctggc aggttatcgc agacagtgta atcagtgcca gaacggcgcg
     1141 accgggcggt aaaaaaggcc aacaggggca ggccactgat gcactgaaca gggccaaaca
     1201 acaggcgggg aatgatgatc cgtacgggga taacataccg ttttaagcaa cgagtgacag
     1261 aagccggagc aatccggctt ttttgtaggt actcctggtg ggggtggcct gtccacgggg
     1321 cgggcggttc gcggaaaaag gcgtattttt tgatttttat gtcaccatca ccaccagttt
     1381 aagttattga tatatggcaa aataaaaatt tttagtgtcg aattaggttg ttttttgttc
     1441 gacgccggaa cgttcccgaa agcatttaca aaaaacaggc gcaaaaaaaa gcgccccgat
     1501 ggtgccaccg gaggcgcttt gtcagcgaca aaggatactg ccactaccga aatgatgagt
     1561 cttaattgtg ctggcgtggc aataattagt caattctttt gctggtatga caaaaatcag
     1621 aaaacacata atctgatttt tagatagaaa atgatttatt taccagtttt atcgatcaat
     1681 aatgatgccc gttaatcata acggaggcag acttatgcca gagaacaaca gcagaaaacc
     1741 ggataaaagt gcaacagtgc acatagacgc cggaactatg gagaagctcg aacgctatca
     1801 gcagttcatt aaaagtcatc accctggaat gccagtaccc acgaaagggc agattgcgag
     1861 aagcgcggtt gaatactggt acagggcaac gttaggagcc tggctatgaa aacatggttt
     1921 tccattaagg ctatggctga tgttgtacat gtacgcattt atgacgagat cggcgggtac
     1981 ggtgtaaaag catcggcact tactgacgag atcaacgcgt gcggtaatgt gcctgaaatc
     2041 catcttcgca tccattcacc tggtggcgac atctttgaag ggctggctat ctataacgcc
     2101 ctgaaaaatc atccggcaaa gaaaattgta cacattgaag gcatggcggc ttctatggcc
     2161 tcgtttattg ccatgtgtgg cgatcacatc gttatgcctg aaaacgcgat gatgatgata
     2221 catgcccccc gtggtgttac tgccggagtg tcgggcgacg ttcgccgctt tgctgacctg
     2281 atggacaagc tgggcgacac gatggcggaa acctacgccg gaagaacggg caggagcaaa
     2341 caggaaatca ccgccatgat ggaggcggaa acctggatgg atggcaatga gtgtaaggct
     2401 aatggcttcg cagatgaggt tatacccgcg attacagcaa tggccagaat tgaatcaaaa
     2461 cgaatcggag atttttcgaa tatgccggaa aatattaaaa gcatgatcag ccagaaaact
     2521 ggcagcggag aacaggaacg actcaacggg atacgtgaat tgtttggcac gttcaatgga
     2581 agatataacg acctggctat aagttgtctt gctgattcag gctgtagcgt tgaggatgca
     2641 cgcgaacgcc ttttactcac tatgggtaaa gaatcgacac cgacaaacaa aaccaccccc
     2701 gcaaatcttt actacgcgta cacggataac ggcaacataa ccggcgatgc aatgcgtcag
     2761 gggcttaatg cgcgtctggg acatgaacag gcagaacgcg gcaaccctta cgccatgatg
     2821 agccttttcg atatggcaca ggcatcatta acccatcgcg gcataagcac aggcagctac
     2881 agcacacgct cgcagatagt aaacgcggca ttcacccaca gcagcagcga ttttacttat
     2941 atccttgctg gtggtgctga aaaatccgtg aacacagcgg cgaaacgttc cgccagtgga
     3001 cgaaaaaagg ttcattgtca aacttccatg aggcacgtcg tgttggcctg aatggcttct
     3061 ctacattaaa caaagtgccg gaaggcgcgg aatataaata catcaccacc agtgaccgtg
     3121 gcgaaccaat cgcgctggca acttacggaa atattttcag cattacccga caggcgatta
     3181 ttaacgatga cctgaatcag ttaacagtta tcccacaggc aatggggcga gcagccgcca
     3241 gaacggtggg taatctggtt catctggtgc tgacaggaaa cacaaagctt tctgacggta
     3301 taccgctgtt cagcaaaaaa cacggaaatg atattgataa cggagtcagc actacagggc
     3361 ttggcattgc acgccgtgca atgcgcacac agagggatga aaacggtgag gtgctgaata
     3421 ttgtgccaaa attccttctt gttcctgcgg ctctggaaga cagagcgttg caaatggtta
     3481 actcaaccac gcctttcggt gccgatacaa acagtggttc atataacccg taccacaaac
     3541 aactggatat tattgtcgat ccccgccttg atgaagtcag tgataagcaa tggtacatgc
     3601 tttcagcaca gggaacggac acaattgagg tggcttatct tgacggtaat gacgagcctt
     3661 accttgagca gcaggagggc tttattgttg acggtgtggc ctggaaggtc cgcattgatg
     3721 ctggcgttgc tgctctggat tatcgcggaa tggtcagagc gatttcttca tgagaacaag
     3781 gcggcagcag ccgccttttt tgcgggtcct cctggtgggg ggggcctgtc cacggggcgg
     3841 gtcggcgcgg aaaaaagcgc atttttgtgg ttttatcgtc atcatcatca tgtgtgtaac
     3901 ctgttgtttt taatgtagtt gatgcaaaaa agatgatgat tgtggttaat ttttgttcga
     3961 catcttttag cgtgacagat tctttacaaa aaatctgagc ttgttttctt aaccagcgcg
     4021 atgggggcac aatgacagaa gccgaaatac tgggattaat ccgccgcgtc gccggaatca
     4081 gccagcaggt tgacgaacag gccatgcaac cggacagcgt gaccgctgaa aattatgcgc
     4141 gtgtggtgga tgaggtaatg cgccgtgacg gtattgagct taacggcgtg gatatgcgca
     4201 acatacgaac cagagttctt gagttgctgg cataccgtcg ccgttctcaa caacggaggg
     4261 agagcgcgaa aaatacttac cagtggagga agccggagca cttacggcgg taactggttg
     4321 atattaccga aagtgcaaaa atgcacttag caactggtgg cgagttgcag atctgcaact
     4381 cgactatgaa actacggaaa ctacccgtag tttgggtagt aagagtaaca cccagatttt
     4441 ggggcttact cgcgataccc aaataaaggg tatcggtgga agaaatatcg tttctcatat
     4501 gtgagtaccg agggcggaat tccgccttcg gttacttatt gtgcgcatgc acagggaggg
     4561 gcgggtcaaa tctctgtacc ctgacgtctt ccggactgcc agccccatcg attttttata
     4621 cccgcgaaaa atgaaaaacg cttcacgctg gtgggcctga tgccgataac tggtttcatg
     4681 actgatagcc ggaaaatgat aacgattaaa gcgtgttgat ctttgttcgt ggttgttcga
     4741 tattgttcgt ggaacggtgt agttaatggt gtagttaatt ttactgtttt tgacgagctt
     4801 attgtttgca actcaatgaa aaataaaggc taataagcca aatgctataa atgatagtta
     4861 tctatcatgt ggagtagatt ggtcaggcaa ataagctctt gtcagcggca gggcgttctg
     4921 ccgataaccg taaccgaaga tgataactga caatgggtaa aacgaacgac tggctggact
     4981 ttgatcaact ggcggaagaa aaagttcgcg acgcgctaaa accgccatct atgtataaag
     5041 tgatattagt caatgatgat tacactccga tggagtttgt tattgacgtg ttacaaaaat
     5101 tcttttctta tgatgtagaa cgtgcaacgc aattgatgct cgctgttcac taccagggga
     5161 aggccatttg cggagtcttt accgccgagg ttgcagaaac caaagtggcg atggtgaaca
     5221 agtacgcgag ggagaatgag catccattgc tgtgtacgct agaaaaagcc tgaatgcagg
     5281 cataaaaatt gggggaggtg cctatgctca atcaagaact ggaactcagt ttaaatatgg
     5341 ctttcgccag agcgcgcgag caccgtcatg agtttatgac cgtcgagcac ttgttactgg
     5401 cgctgctcag taacccatct gcccgggagg cgctggaagc gtgttctgtg gatttggttg
     5461 cgctccgtca ggaactggaa gcctttattg aacaaaccac acccgttctg cctgccagtg
     5521 aagaggagcg cgacacacag ccgacgctga gttttcagcg tgtactgcaa cgtgcggtct
     5581 tccatgtcca gtcctccggt cgcaatgagg ttaccggtgc aaacgttctg gtcgctatct
     5641 ttagcgaaca ggagtcgcag gcggcatatc tgttgcgtaa acatgaagtc agccgtctcg
     5701 atgtggtgaa ttttatctct catggcacgc gtaaagacga gccgacacag tcttctgatc
     5761 ctggcagcca gccaaacagc gaagaacaag ctggtgggga ggaacgtatg gagaatttca
     5821 cgacgaacct gaatcagctt gcgcgcgtgg gcggaatcga cccactgatt ggtcgtgaga
     5881 aggagctgga gcgtgctatt caggttctct gccgtcgccg taaaaacaac ccgctgctgg
     5941 tgggggaatc tggtgtcggt aaaaccgcga ttgcggaagg tcttgcctgg cgaattgttc
     6001 agggcgatgt gccggaagtg atggctgact gtacgattta ctctctcgat atcggttctc
     6061 tgttagcggg cacaaaatat cgcggcgact ttgaaaaacg ttttaaagcg ttgctcaagc
     6121 agctggagca ggacactaac agcatcctgt ttattgatga gatccacacc attatcggtg
     6181 cgggggcagc gtctggtggt caggttgatg cggctaacct aatcaaaccg ttgctctcca
     6241 gcggtaaaat tcgtgtaatt ggttcgacaa cctatcagga gttcagcaac attttcgaga
     6301 aagaccgtgc tctggcgcgt cgcttccaga aaattgatat tactgaaccg tcaatcgaag
     6361 aaactgttca aatcatcaat ggcctgaaac cgaagtatga agcgcaccac gacgtgcgtt
     6421 ataccgcaaa agcggtgcgt gcggcggtag agctggcggt gaaatacatc aatgatcgtc
     6481 atctgccgga taaagcgatt gatgttatcg acgaagcggg cgctcgcgca cgcctgatgc
     6541 cggtaagcaa acgcaagaaa accgttaatg tggcggatat tgagtccgtg gtggcccgta
     6601 ttgcacgcat tccagagaag agtgtttctc agagtgaccg cgataccctg aaaaacctcg
     6661 gcgatcgcct gaaaatgctg gtcttcggtc aggataaagc cattgaggcg ctgactgaag
     6721 ccattaagat ggcgcgtgca ggtttaggtc acgaacataa accggtcggt tcgttcctgt
     6781 ttgccggtcc taccggggtc gggaaaacag aggtgacggt acagctttcg aaagcgttgg
     6841 gcattgagct tctgcgcttt gatatgtccg agtatatgga acgccatacc gtcagccgtc
     6901 tgattggtgc gcctccggga tacgttggtt ttgatcaggg cggtttgctg accgatgcgg
     6961 taatcaagca tccacatgcg gtgctgctgc tggacgaaat cgagaaagcg catccggacg
     7021 tgttcaatat tctgttgcag gtgatggaca acggtacgct gaccgataac aacggacgca
     7081 aagcggactt ccgtaacgtg gtgctggtga tgaccaccaa cgccggggta cgggaaactg
     7141 agcgcaaatc cattggtctt atccaccagg ataacagcac cgatgcgatg gaagagatca
     7201 agaagatctt tacaccggaa ttccgtaacc gtctcgacaa cattatctgg tttgatcatc
     7261 tgtcaaccga cgtgatccat caggtggtgg ataaattcat cgtcgagttg caggttcagc
     7321 tggatcagaa aggtgtttct ctggaagtga gccaggaagc gcgtaactgg ctggccgaga
     7381 aaggttacga ccgggcaatg ggcgctcgtc cgatggcgcg tgtcatccag gacaacctga
     7441 aaaaaccgct cgccaacgaa ctgctgtttg gttcgctggt ggacggcggt caggtcaccg
     7501 tcgcgctgga taaagagaaa aatgagctga cttacggatt ccagagtgca caaaagcaca
     7561 aggcggaagc agcgcattaa tctaattgtt aggtaggttg gtgaagtccg taatctcgaa
     7621 agaggttacg gactttttgt ttatggggtg gaacggtgaa aaccctattt ttggaggtga
     7681 aggtaatttg ttgataatta gtgctgctgg aaaaggtaag gataaaaaag ggtgacagca
     7741 ggaaaatgag atggttttgc tttattaaca acggactaaa cgtgtagtat ttgagttcac
     7801 tgccgtacag gcagcttaga aatcgacggg gtgcggtaaa acctttgcga acgcgttcac
     7861 tgccgtacag gcagcttaga aattcacagg taacatactc cacccgccca ccatgttcac
     7921 tgccgtacag aaagataaaa tgcgaaaaaa aagctcgcac tttcgtacga gctcttcttt
     7981 aaatatggcg gtgagggggg gattcgaacc cccgatacgt tgccgtatac acactttcca
     8041 ggcgtgctcc ttcagccact cggacacctc accaaattgt tttgctacca aacctcatgg
     8101 gtggcaacgg ggcgctacta tagggagttg gagtaaaacg gtcaagaaga attttaatga
     8161 taattattgt ttgctcatac tgtaaacaag ttgtgcagta tatctacatc gagacaagtt
     8221 acggacttat acttccaaag tacttcatac atatcacaaa ataaaaaggc cggttaaacc
     8281 gaccttttac tcgttctttc tcttcgccca tcaggcggta aaacaatcag cgactacgga
     8341 agacaatgcg gcctttgctc aggtcgtacg gggtcagttc aacagtcact ttgtcgcccg
     8401 tcaggatgcg gatgtagttt ttgcgcattt taccggagat gtgtgcagta accacgtgac
     8461 cgttttctaa ctctacgcgg aacatggtat taggcaacgt ttcaagaacg gtaccttgca
     8521 tttcaatatt gtcttctttg gccatctaat cctctggggt atcactaccg taatttgaac
     8581 cggcaagata atgccgaagt tctgtaaata agtaaagatt tgcgcgctaa atcgcaacaa
     8641 acaggttcgg cacattactc cgaaaacaca cggctaagcc gcaccaaaag cgcaacgtat
     8701 aagggagcgg tgagataaac gatgggcgtt acctgacgcg aaaaattcct tatcggcagc
     8761 ggggtaatga gcataaccaa ctctgcgacc gcaattataa cactctgggg agaaatgtgc
     8821 cgaaaacatt catttttgtg gtgaaaacaa gcatcgtggc acccagaaat tattcggcaa
     8881 tcgtccgagg cgcatttgat tgagataatt cagataatcc cgacggggaa tttcgcaggc
     8941 accaagcgat gctgtgtgat cgttaaggac ctggcagtcg ataagcttac cgccatgact
     9001 gataaattcc tcacagaata ccagaagcgc cgttttagac gcattttcca tccggctgaa
     9061 catggactcg ccacaaaata gcgttccctg ggccacgccg tacataccgc cgacaagctc
     9121 atcttcatgc cagacttcaa tggagtgggc atgaccgagt tcgtgaaggc gatggtaggc
     9181 ttcgaccacg ccacgcgtga tccaggttcc ttcttcgcga tcgctggcac aaccttcaat
     9241 gacctgacca aaagcgtaat tcatcgtgac acgatagggc gagcgtttat gaaatcgctt
     9301 catactacgg ctgatatgca gtgattcagg ccaaagcacc gcgcgaggat cgggcgacca
     9361 ccagaggatg ggggcgcctg gagaaaacca cggaaaaata ccacgctggt aagccattaa
     9421 caggcgcgca gggctaagat cgccccccag agccagcagg ccgttaggct cacgtaatgc
     9481 gccttccggg gaagggaagg ctattgaatg gcgagaaagc tgaaccaggc gcatgaccgc
     9541 aaaactccac gcaagtcgga tcgttcaata atagcttaca aaccctgctt aaactggtaa
     9601 taacgcccct gtctggcaag cagttctgcg tgagtacctt gctcaataat ttgcccgttg
     9661 tccatcacta ttatttgttg gaaacgagag agtccgcgaa gtcgatgggt gaccattaac
     9721 accgttttct cacgcatcat ttctgcaagc aattcaagga tctggctttc ggttgtggca
     9781 tctaagcctt cggtaggttc atccagcaac accagtggcg catcatgtaa cagcgcacgg
     9841 gcgatagcca gacggcgcag ttcaccaccg gagagctggc gtccgccttc acctaaccaa
     9901 ctgttgagac ctgcgtcctc gagcagcttc tccagaccaa cgcgacgcaa gatctccgac
     9961 agagcctcat cactactgcc aggcgaggcg agtaacagat tatcacgcag cgtggcgcta
    10021 aacagatgca ctcgctgagg aacaacgctg atggtctgtc gtagagccgc ttcattcagg
    10081 ctggctatgg ggctatcgtt aagcaaaatc tcgccctgtt gcgggtccca agcgcgggtc
    10141 agcagttgta acagtgttga tttgccgcat ccggttcgcc cgagaatcgc aatatgttcc
    10201 ccggcgttta cctgaagaga aatcccttta agtgcctgtt gggattgctc cggataagtg
    10261 aactgaacat cccgtaatgt cagcgaaacg cgatcggcaa cacgagtttg ggtatcaggg
    10321 aaggtgactt ccggtttttg atccgttaaa tcagtgatcc gaacggcaga ggcaatgact
    10381 tgccccagat gctgaaatgc acccgttact ggtgccagtg cttcaaacgc ggctaacgcg
    10441 cagaagacaa acagggcaat taacgcgccg ggttgagcat tgccgccaac gccgccagac
    10501 gccatccaca gcatcaggat caccgctaac gcgccaatga gcagcattat cgcttgcgac
    10561 aatgcggtca gttcagattg acggcgttgc gcttccagcc attgaatttc tgtattctct
    10621 agttgcgtgc gataacgatc gctggcacca aaaatggtca gctcagcttg cccttgcagc
    10681 caggccgtca gttgttggcg atactgtccg cgaagatgag tcagattttg cccggtgctt
    10741 tttcccgcac gataaaacag cggtggcatc aggaaaagcg tcagtaacat aatgccgccc
    10801 agcgtaaagg cgagggtgaa atcaaggaaa cttaacccga ctgtcaccac cataatcacc
    10861 acaaaagcgc ccaccagcgg cgagataacg cgcaggtaaa gatgatcgag cgtatcaaca
    10921 tccgccacca cgcgattgag caattcgccc tgacgatagc gcgccagtcc ggcaggggag
    10981 aggggcaaca atttgctgaa ggtgtaaatg cgcagatgct gcaacacgcg gaaagtcgcg
    11041 tcgtgactta ccagacgttc aaaataacgc ccggcagtac gggtgattgc ggcaccacgc
    11101 acgcccgcag cgggtagcat atagttgaag ctgtacagtc cggcaccccc cgcaaccgct
    11161 gaggccgaga ggaaccagcc ggaaagtgtc aacagaccga tactggcgag cagcgtcaca
    11221 attgccagca caataccaag acttaacatc catttatgac gtttatacag tgccagatag
    11281 ggtagcaaag cgcgcattta aatctcctcc tgacgatggg ccagtaatgt ggcgaatggg
    11341 ccaccagcca cacttaattc cgcgtaacgt ccttgctcaa taatctgacc atcctgcatt
    11401 acccaaatga catcccagtc agcaagatct tctaactggt gggtgaccat taacgttgtc
    11461 tggcgcagag aggcggcatt cagcgcctcc attacgcgct gttcactgtg agcatcaagg
    11521 ctggcagcgg gttcatccaa cagtaatagc gaacagggat ttagtaacgc acgggccacc
    11581 gccacgcgct gcgcctgccc cacggaaagg cgggcagcct ggtcgccaac aggcgtatca
    11641 acgccttgtg gcaggagcgg tagaaactcg ctgacccagg cgttatccag cgctgcttgt
    11701 aattcatgtt cgctggcatc aggtcgcgcc agtagtacgt tatcccgtaa tgttgctgcc
    11761 ggtaattgtg gattttgccc aacccaggag agatgtttac gccatgattc tggtgataaa
    11821 tcgcgtaatt ctatcccgtt gattcgtagc gatccctgat atgagagaaa accagaaagc
    11881 gcgttcaaca gtgaactttt acctgaaccg ctgcgaccaa ccaacacggc acgttggcct
    11941 gctggcaaag taaagttcag cggtccggcc agcgttttac cttccggcga cgtgataaac
    12001 agatcctcgg cctcaatggt caccggatcg gtcgatgcta attccacctc gccacgctgc
    12061 ggatgggcga gcggggtttc cataaacgtt ttcagactgt cagctgcacc aacagcctgg
    12121 gctttagcat gataaaacgt accgagatcg cgtaatggct ggaaaaactc tggcgcaagg
    12181 atcagggcca gaaaacccgc agccagcgtc acgccagtat cgtagtgacc aaaatccagc
    12241 tcgccgagat aggaaaaacc aaagtagacc gccaccagag caattgacag cgaggtaaaa
    12301 aattcgagaa tgccggagga taaaaatgcc agccgtagca cttccattgt ccgttggcgg
    12361 aaatcttccg aagcagaacg aatactttca atttcagctt caccacgacc aaaaatacgc
    12421 aatgtttcca tgccgcgcag gcgatcgagg aaatgcccac ttaagcgagc aagagcgaga
    12481 aagttacgtc ggttagcatc ggcagccccc attccaacca gcgccataaa caacggaatt
    12541 aacggtgcag tgcccagcag aatgagcgcc gcagcccagt ttgaggggaa gattgccacc
    12601 acaatcagca acggcaccga cactgccagc gccatttgcg gcaggtagcg tgcatagtaa
    12661 tcatgcatat cgtcaatttg ctcgagtacc agcgtcgccc agctccccgc aggtttaccc
    12721 tgaatccacg ctggccctgc ttgttgcaga cggtcgagaa cctgacggcg gatggcaaag
    12781 cggatatgct gcccggcgtg ataacccacc cgttcgcgta accagaccgc ccatgcgcgc
    12841 agtacaaagg tcagaaccag taacgtaaag ggaagcagca gggcttcacg gggaatattc
    12901 tccataatca tatgttgcag aattcgcgcc atgaaccagg cctgggcaat gatcaatatg
    12961 ccgcttacaa agcccagcag acgagaaata ttcagccaac gttgggagat gacgctttgc
    13021 tgttttaacc agcgggttaa ctctttttga cgagatttat tcattgcacg cttagcaggt
    13081 gagttatcag aattatttgc agagcaatgt tacaacgggg aaaaaataaa ggcgacccat
    13141 agtcgcaagg tgtcgccttc tttacttttg ttactgattt gtaaaattat tttgcgtcag
    13201 ctaaaccatc gaggtagcgt tccgcatcaa gtgctgccat gcagccagta ccggccgaag
    13261 taatggcctg gcgataaatg tgatccatca cgtcgcctgc ggcaaagacg ccaggaatgc
    13321 tggtctgggt ggcattacca tgaatacccg actgtacttt gatgtagccg ttttccagtt
    13381 ccagctgccc ttcgaaaatc gcagtattcg ggctgtgacc gatagcaaca aacagaccgg
    13441 caacgtcgag tgactcgatg ttatcgctgt tttgcgtatc gcgcagacga acgccagtga
    13501 cacccatttg atcgccggtc acttcttcca gcgtacggtt ggtgtgcaga atgatgttgc
    13561 cgttctccac tttatccatc agacgcttaa taaggatttt ttccgcgcgg aaaccgtcac
    13621 ggcggtgaat cagatgcact tccgaagcga tgttagacag atacagcgcc tcttcaaccg
    13681 cggtattgcc gccgccgatg accgcaactt tctggttgcg atagaagaaa ccgtcgcagg
    13741 ttgcacaagc agaaacccca cggcctttaa aggcttcttc agagggcagg ccgagatagc
    13801 gtgcagaagc tccggtggca ataatcagcg cgtcgcaagt gtattcgccg ttatcgccat
    13861 tcagacggaa cggacggttt tgcagatcca ccttgttgat atgatcaaaa atgatctcag
    13921 tttcaaactt ggtggcatgt tcgtgcatgc gctccattaa taacggaccg gtcagatcgt
    13981 ttggatcacc aggccagttt tccacttccg tggtggtggt cagttggccg cctttttcca
    14041 tgccggtaat cagcacaggt tgcaggttgg cgcgcgccgc gtagacagca gcggtgtatc
    14101 ccgccgggcc tgaacccagg ataagcagtt tactgtgttt ggtcgtgccc atgagatccc
    14161 catagttgtt ggcagacaat gggcaggatt gtagggaatt tacagacgta aaaaaagagt
    14221 atgacgattt tgttaacaat ttgtgcaatc ggcagcatcg ataagcaggt caaattctcc
    14281 cgtcattatc acctctgcta cttaaatttc ccgctttata agccgattaa atgatgaata
    14341 aacgcccctg ttaatgaata tctggcatgt tgtactaaaa atcgatgttt tgctttgaca
    14401 atcccctggt gttttgcgaa aacattcgag gaagaaaaaa aacagtattc ttatatgcgc
    14461 ataaccatgc atgtaaatac catgtttacc gtgctagtga aatctacgta tggcgtggac
    14521 agacgccatt cgtgatgtcg atagctgcca caaggcaacg gtcttctcac cgtagaccca
    14581 ggcattgcgc gccgtgaatc ttcatgattt cggtctatcg tgacgggtag cgactctgaa
    14641 cagtgatgtt tcaggggcag acaggagtag ggaaggaata cagagagaca ataataatgg
    14701 tagatagcaa gaagcgcctt ggcaaagatc tcgaccgtat cgatcgtaac attcttaatg
    14761 agttgcaaaa ggatgggcgt atttctaacg tcgagctttc taaacgtgtg ggactttccc
    14821 caacgccgtg ccttgagcgt gtgcgtcggc tggaaagaca agggtttatt cagggctata
    14881 cggcgctgct taacccccat tatctggatg catcacttct ggtattcgtt gagattactc
    14941 tgaatcgtgg cgcaccggat gtgtttgaac aattcaatac cgctgtacaa aaacttgaag
    15001 aaattcagga gtgtcattta gtatctggtg atttcgacta cctgttgaaa acacgcgtgc
    15061 cggatatgtc agcttaccgt aagttgctgg gggaaaccct gctgcgtctg cctggcgtca
    15121 atgacacacg gacatacgtt gttatggaag aagtcaagca gagtaatcgt ctggttatta
    15181 agacgcgcta acacggaaca ggtgcaaaat cggcgtattt tgattacact cctgttaatc
    15241 catacagcaa cagtactggg gtaacctggt actgttgtcc gttttagcat cgggcaggaa
    15301 aagcctgtaa cctggagagc ctttcttgag ccaggaatac actgaagaca aagaagtcac
    15361 attgacaaag ttaagtagcg gccgccgcct tctggaggcg ttgctgatcc ttattgtcct
    15421 gtttgccgtc tggttgatgg ctgccttact aagctttaac ccttcggacc ccagctggtc
    15481 gcaaacggcc tggcatgaac ctatccataa tttaggtggg atgcccggtg cgtggttggc
    15541 agatacgctg ttctttattt ttggcgtgat ggcttacacc attcccgtca ttattgtcgg
    15601 cggttgttgg tttgcctggc gtcatcagtc cagcgacgaa tacattgatt attttgccgt
    15661 ttcgctacgc atcattggcg ttttggcgct catccttacc tcctgtggtc tggcggcaat
    15721 caacgctgac gatatctggt attttgcctc cggtggcgtc attggcagct tactaagcac
    15781 tacgctacaa ccactgctac acagtagcgg gggaactatt gcgctgctct gcgtttgggc
    15841 agcgggcctg acgttgttca ccggttggtc atgggtgacc attgctgaaa aactcggcgg
    15901 ctggatttta aacattctca ccttcgccag taatcgtacc cgtcgcgatg atacctgggt
    15961 cgatgaagat gagtatgaag acgacgaaga gtatgaagat gaaaatcacg gcaaacagca
    16021 tgaatcacgc cgtgcccgta ttcttcgcgg cgcgctagcg cgtcgtaaac ggttggcgga
    16081 aaaattcatt aatccgatgg ggcggcaaac agacgctgcg ttgttctccg gtaagcggat
    16141 ggatgatgac gaagagatta cctacactgc acgcggtgtg gctgccgacc ctgacgacgt
    16201 cctattttcg ggcaatcgtg caacgcagcc agaatatgac gaatacgatc cattattaaa
    16261 cggtgcgcca attaccgaac ctgtcgctgt agcagctgct gctaccacgg cggcacaaag
    16321 ctgggctgcg ccggttgaac ctgtgactca gacgccgcct gttgcctctg ttgatgttcc
    16381 accttcgcaa cctacagtag cctggcagcc tgtaccgggt ccacaaacgg gagagccggt
    16441 tattgctcct gcaccggaag gttacccaca gcagccacaa tatgcgcagc ctgcagtgca
    16501 atataatgag ccgctgcaac aaccagtaca gccacagcag ccgtattatg cacctgcagc
    16561 tgaacaacct gtgcaacaac cttactacgc tcctgcggcg gaacaacctg ttcagcaacc
    16621 gtattacgcc cctgcgccag aacaaccggt ggcaggtaac gcctggcaag ccgaagagca
    16681 gcaatccact tttgctccac agtctacata ccagactgag caaacttatc agcaaccagc
    16741 cgctcaggag ccgttgtacc aacagccgca acccgttgaa cagcagcctg ttgtggagcc
    16801 tgaacccgtt gtagaagaga caaaacccgc gcgtccgcca ctttactatt ttgaagaagt
    16861 ggaagagaag cgagcccgtg aacgtgaaca acttgcggcc tggtatcaac cgattccaga
    16921 accggttaaa gaaccagaac cgatcaaatc ttcgctgaaa gcaccttctg ttgcagcagt
    16981 acctccagta gaaaccgctg ccgctgtttc tccgctggca tctggcgtga aaaaagcgac
    17041 actggcgacg ggggctgccg caaccgttgc cgcgccagtc ttcagtctgg caaatagcgg
    17101 tggaccgcgt ccgcaggtca aagaggggat tggcccgcag ttgccacgac cgaaacgtat
    17161 ccgcgtgcca actcgccgtg aattggcgtc ttacggtatt aagctgccct cacagcgtgc
    17221 ggcggaagaa aaagcccgtg aagcccagcg caatcagtac gattctggcg atcagtacaa
    17281 cgatgatgaa atcgatgcga tgcagcagga tgaactggca cgtcagttcg cccagacaca
    17341 gcagcaacgc tatggcgaac agtatcaaca tgatgtgccc gtaaacgcag aagatgcaga
    17401 tgctgcggca gaggctgaac tggctcgtca gtttgcgcaa actcaacaac aacgttattc
    17461 cggcgaacaa ccggctgggg cgaatccgtt ctcgctggat gattttgaat tttcgccaat
    17521 gaaagcgttg ctggatgatg gtccacacga accgttgttt acgccaattg ttgaacctgt
    17581 acagcagccg caacaaccgg ttgcaccgca gcagcagtat cagcagccac aacagccggt
    17641 tgcgccacag cagcaatatc agcagccgca acagccggtt gcgccgcagc cacaatatca
    17701 gcagccgcaa caaccggttg cgccacagca gcaatatcag cagccgcaac aaccagttgc
    17761 gccgcagccg cagtatcaac agccgcaaca accggttgcg ccgcagcagc agtatcagca
    17821 gccacaacag ccagttgcgc cacaaccgca ggataccctg cttcatccgc tgttgatgcg
    17881 taatggcgac agccgtccgt tgcataaacc gacgacgccg ctgccttctc tggatttgct
    17941 gacaccgccg ccgagcgaag tggagccggt agataccttt gcgcttgaac aaatggctcg
    18001 cctggtggaa gcgcgtctgg ctgatttccg tattaaagcc gatgttgtca attactctcc
    18061 ggggccggtt atcactcgct ttgaattgaa cctggcaccg ggcgtaaaag cggcgcgcat
    18121 ttctaacttg tcacgggacc ttgcccgttc actttcgacg gtggcggtgc gtgtcgttga
    18181 agttattcct ggcaaaccct atgtaggtct ggagttaccg aataaaaaac gacaaaccgt
    18241 ttatctgcgc gaagttttgg ataacgccaa attccgcgat aatccgtcgc cattaaccgt
    18301 ggtgctgggt aaagatatcg ccggtgagcc ggtggttgcc gatctggcga aaatgccgca
    18361 cttgttggtt gcggggacta ccggttccgg taaatctgtc ggtgtgaacg cgatgatcct
    18421 gagcatgctt tataaagcac agccagaaga tgtgcgtttc atcatgatcg acccgaaaat
    18481 gctggagctt tcggtttatg aaggcattcc gcatctgtta acggaagtcg ttactgatat
    18541 gaaagatgcc gccaacgcgc tgcgctggtg tgttaacgag atggagcgtc gttataaact
    18601 gatgtctgcg ctgggtgtgc gtaatctggc gggttataac gaaaaaattg ctgaagccga
    18661 tcgcatgatg cgtccgattc cagacccgta ctggaagccg ggtgacagta tggatgccca
    18721 gcatccggtg ctgaaaaaag aaccatacat tgtggtgttg gttgacgaat ttgccgacct
    18781 gatgatgacg gtaggtaaaa aagtggaaga gctgatagca cgtctggcgc aaaaagcccg
    18841 tgccgcgggt atccacctcg tactggcaac tcagcgtcca tcggttgatg ttattactgg
    18901 tctgattaaa gcgaatattc cgacccgtat cgcctttacc gtatccagta agattgactc
    18961 acgtaccatt cttgatcagg ctggcgcgga atcactgctg gggatggggg atatgctcta
    19021 ctctgggccg aactccacgt tgccggtacg tgtccatggt gcttttgttc gcgatcagga
    19081 agttcatgcc gtggtgcagg actggaaagc gcgtggtcgc ccacagtatg ttgatggcat
    19141 cacttccgac agtgaaagcg aaggtggcgc gggcggtttc gatggcgctg aagaactgga
    19201 tccgttgttc gatcaggcgg tgcagtttgt cactgaaaaa cgcaaagcgt caatttctgg
    19261 cgtacagcgt cagttccgca ttggttataa ccgtgcagcg cgtattatcg aacagatgga
    19321 agcgcagggg attgtcagcg aacaggggca caacggtaat cgtgaagtgc tggctccacc
    19381 gccgtttgac taattaatgc attgccggat aaggcgcggt agcgtcgcat ccggcactct
    19441 atcaactgaa aattcagtat tttcttcttt cctcaagctg attattagcc tggaatagag
    19501 agtagaggga actcccgatc gggagtgacg taatttgagg aataatgatg aaaaaaattg
    19561 ccatcacctg tgcattactc tcaagcttag tagcaagcag cgtttgggct gatgccgcaa
    19621 gcgatctgaa aagccgcctg gataaagtca gcagcttcca cgccagcttc acacaaaaag
    19681 tgactgacgg tagcggcgcg gcggtgcagg aaggtcaggg cgatctgtgg gtgaaacgtc
    19741 caaacttatt caactggcat atgacacaac ctgatgaaag cattctggtt tctgacggta
    19801 aaacactgtg gttctataac ccgttcgttg agcaagctac ggcaacttgg ctgaaagatg
    19861 ccaccggtaa tacgccgttt atgctgattg cccgcaacca gtccagcgac tggcagcagt
    19921 acaatatcaa acagaatggc gatgactttg tcctgacgcc gaaagccagc aatggcaatc
    19981 tgaagcagtt caccattaac gtgggacgtg atggcacaat ccatcagttt agcgcggtgg
    20041 agcaggacga tcagcgcagc agttatcaac tgaaatccca gcaaaatggg gctgtggatg
    20101 cagcgaaatt taccttcacc ccgccgcaag gcgtcacggt agatgatcaa cgtaagtaga
    20161 ggcacctgag tgagcaatct gtcgctcgat ttttcggata atacttttca acctctggcc
    20221 gcgcgtatgc ggccagaaaa tttagcacag tatatcggcc agcaacattt gctggctgcg
    20281 gggaagccgt tgccgcgcgc tatcgaagcc gggcatctgc attctatgat cctctggggg
    20341 ccaccgggta ccggcaaaac aaccctcgct gaagtgattg cccgctatgc gaacgctgat
    20401 gtggaacgta tttctgccgt cacctctggc gtgaaagaga ttcgcgaggc gatcgagcgc
    20461 gcccggcaaa accgcaatgc aggtcgccgc actattcttt ttgttgacga agtgcaccga
    20521 ttcaacaaaa gccagcagga tgcatttctg ccacatattg aagacggcac catcactttt
    20581 attggcgcaa ccactgaaaa cccgtcgttt gagcttaatt cggcactgct ttcccgtgcc
    20641 cgtgtctatc tgttgaaatc cctgggtaca gaggatattg agcaagtact aactcaggcg
    20701 atggaagaca agacccgtgg ttatggtggt caggatattg ttctgccaga tgaaacacga
    20761 cgcgccattg ctgaactggt gaatggcgac gcgcgccggg cgttaaatac gctggaaatg
    20821 atggcggata tggccgaagt caatgatagc ggtaagcggg tcctgaagcc tgaattattg
    20881 accgaaatcg ccggtgagcg aagcgcccgc tttgataaca aaggcgatcg cttttacgat
    20941 ctcatttccg cactgcataa gtcggtacgt ggtagcgcac ccgatgcggc gctctactgg
    21001 tatgcgcgaa ttattaccgc tggtggcgat ccgttatatg tcgcgcgtcg ttgcctggca
    21061 attgcttccg aagatgtcgg caatgccgac ccacgggcga tgcaggtggc aattgcggcc
    21121 tgggattgct ttactcgcgt tggcccggcg gaaggtgaac gcgccattgc tcaggcgatt
    21181 gtttacctgg cctgcgcgcc aaaaagcaac gctgtctaca ctgcgtttaa agctgcgctg
    21241 gcggatgctc gcgaacgccc ggattatgac gtgccggttc atttgcgtaa tgcgccgacg
    21301 aaattaatga aggaaatggg ctacgggcag gaatatcgtt acgctcatga tgaagcaaac
    21361 gcttatgctg ccggtgaggt ttacttcccg ccggaaatag cacaaacacg ctattatttc
    21421 ccgacaaaca ggggccttga aggcaagatt ggcgaaaagc tcgcctggct ggctgaacag
    21481 gatcaaaata gccccataaa acgctaccgt taatgttatc gttgcggtaa tgttgttact
    21541 gtatccctgt ggtcgcaggc tgtggccaca tctcccattt aattcgataa gcacaggata
    21601 agcatgctcg atcccaatct gctgcgtaat gagccagacg cagtcgctga aaaactggca
    21661 cgccggggct ttaagctgga tgtagataag ctgggcgctc ttgaagagcg tcgtaaagta
    21721 ttgcaggtca aaacggaaaa cctgcaagcg gagcgtaact cccgatcgaa atccattggc
    21781 caggcgaaag cgcgcgggga agatatcgag cctttacgtc tggaagtgaa caaactgggc
    21841 gaagagctgg atgcagcaaa agccgagctg gatgctttac aggctgaaat tcgcgatatc
    21901 gcgctgacca tccctaacct gcctgcagat gaagtgccgg taggtaaaga cgaaaatgac
    21961 aacgttgaag tcagccgctg gggtaccccg cgtgagtttg actttgaagt tcgtgaccac
    22021 gtgacgctgg gtgaaatgca ctctggcctc gactttgcag ctgcagttaa gctgactggt
    22081 tcccgctttg tggtaatgaa agggcagatt gctcgcatgc accgcgcact gtcgcagttt
    22141 atgctggatc tgcataccga acagcatggc tacagtgaga actatgttcc gtacctggtt
    22201 aaccaggaca cgctgtacgg tacgggtcag ctgccgaaat ttgctggcga tctgttccat
    22261 actcgtccgc tggaagaaga agcagacacc agtaactatg cgctgatccc aacggcagaa
    22321 gttccgctga ctaacctggt gcgcggtgaa atcatcgatg aagatgatct gccaattaag
    22381 atgaccgccc acaccccatg cttccgttct gaagccggtt catatggtcg tgacacccgt
    22441 ggtctgatcc gtatgcacca gttcgacaaa gttgaaatgg tgcagatcgt gcgcccggaa
    22501 gactcaatgg cggcgctgga agagatgact ggtcatgcag aaaaagtcct gcagttgctg
    22561 ggcctgccgt accgtaaaat catcctttgc actggcgaca tgggctttgg cgcttgcaaa
    22621 acttacgacc tggaagtatg gatcccggca cagaacacct accgtgagat ctcttcctgc
    22681 tccaacgttt gggatttcca ggcacgtcgt atgcaggcac gttgccgcag caagtcggac
    22741 aagaaaaccc gtctggttca taccctgaac ggttctggtc tggctgttgg tcgtacgctg
    22801 gttgcagtaa tggaaaacta tcagcaggct gatggtcgta ttgaagtacc agaagttctg
    22861 cgtccgtata tgaacggact ggaatatatt ggctaatacc caatttttct gaatctaaaa
    22921 agcgcctgcg ggcgcttttt ttgtctccct ttgataccga acaataatta ctcttcactt
    22981 acacgtaata ctactttcga gtgaaaatct acctatctct ttgattttca aattattcga
    23041 tgtatacaag cctatatagc gaactgctat agaaataatt acacaatacg gtttgttact
    23101 ggaatcaatc gtgagcaagc ttgagtgagc cattatgaaa acgaaaatcc ctgatgcggt
    23161 attggctgct gaggtgagtc gccgtggttt ggtaaaaacg acagcgatcg gcggcctggc
    23221 aatggccagc agcgcattaa cattaccttt tagtcggatt gcgcacgctg tcgatagcgc
    23281 cattccaaca aaatcagacg aaaaggttat ctggagcgcc tgtacagtta actgtggtag
    23341 tcgctgcccg ctacgtatgc acgtcgtgga cggtgaaatc aaatatgtcg aaacggacaa
    23401 taccggcgat gacaattacg acggcctgca ccaggttcgc gcctgcctgc gtgggcgttc
    23461 catgcgtcgc cgtgtctaca atccggaccg cctgaaatat ccgatgaaac gagtcggggc
    23521 gcgcggtgaa ggcaaattcg agcgcattag ctgggaagaa gcctacgaca tcatcgcgac
    23581 caatatgcag cgcctgatca aagagtacgg caacgagtct atctatctga actatggcac
    23641 cggtacgctg ggcggcacca tgacccgctc ctggccgccg ggaaataccc tggtcgcgcg
    23701 gctgatgaac tgctgcggcg gctatctgaa ccattacggc gactactcct ccgcgcaaat
    23761 tgcggaaggt ttgaactata cctacggcgg ctgggcagat ggcaacagcc cgtcggatat
    23821 cgaaaacagt aagctggtag tgctgtttgg taataaccct ggcgaaacgc gaatgagtgg
    23881 cggtggggtg acttactatc ttgaacaggc acgccagaaa tctaatgccc gcatgatcat
    23941 catcgatccg cgctataccg acaccggtgc cgggcgcgaa gatgagtgga tccctattcg
    24001 tccgggaaca gatgccgcac tggttaacgg tctggcgtac gtcatgatca ctgaaaacct
    24061 ggtggatcag gcattcctcg ataaatattg cgttggctac gatgagaaaa ccctgccagc
    24121 cagtgcgccg aaaaatggcc actataaagc ttatattctg ggtgaagggc cagatggcgt
    24181 ggctaaaaca ccggaatggg cctcacaaat caccggtgtt ccggcagaca aaatcatcaa
    24241 actggctcgt gaaatcggca gtaccaaacc ggcgtttatc agccagggat ggggcccgca
    24301 gcgtcacgct aacggtgaaa tcgcaacccg tgctatctcg atgctggcga ttctgaccgg
    24361 taacgttggt attaatggag ggaacagcgg cgcgcgtgaa ggttcataca gcttaccgtt
    24421 tgtccgtatg ccgaccttgg aaaacccgat ccagaccagc atttcgatgt ttatgtggac
    24481 cgatgccatt gaacgtggcc cggaaatgac ggcgctgcgt gatggtgtac gcgggaaaga
    24541 taagctggat gtgccgatca aaatgatctg gaactatgcc ggtaactgcc tggttaacca
    24601 gcattctgaa atcaaccgta cccatgaaat ccttcaggat gataagaagt gcgagctgat
    24661 tgtggttatc gactgccaca tgacctcatc ggcgaaatat gctgacatcc tgctgcctga
    24721 ctgcaccgct tccgaacaga tggactttgc gctggatgca tcctgcggga atatgtctta
    24781 cgtgattttc aacgatcagg tgattaaacc gcgctttgaa tgtaagacca tctatgaaat
    24841 gaccagcgaa ctggcaaaac gtcttggcgt tgagcaacag tttactgaag gccgtaccca
    24901 ggaagagtgg atgcggcatc tgtatgccca gtcgcgggaa gcgattcctg aactgccaac
    24961 gtttgaagag ttccgcaagc aggggatctt taaaaagcgc gacccacaag ggcatcacgt
    25021 tgcttataaa gccttccgtg aagatccgca ggcaaaccca ctgactacgc catcgggcaa
    25081 aattgagatt tattcgcagg cgctggctga cattgccgct acctgggaat tgccagaagg
    25141 cgatgtgatc gatccactgc cgatctacac gccgggcttt gaaagttatc aggatccgct
    25201 gaacaaacag tatccgctgc agcttacggg tttccactat aaatctcgcg ttcactcaac
    25261 ttacggcaac gttgatgtgc tgaaagcagc ttgccgtcag gaaatgtgga tcaacccgct
    25321 tgatgcgcaa aaacgcggta tcaacaacgg cgataaagtc aggatcttta acgatcgtgg
    25381 tgaagttcat attgaggcga aagtgacgcc acgaatgatg ccaggtgtgg tcgcactggg
    25441 tgaaggtgcc tggtatgacc cggatgcaaa acgtgtcgat aagggtggtt gtattaacgt
    25501 actgaccact caacgtccgt ctcctctcgc taaggggaat ccgtcacata caaaccttgt
    25561 tcaggttgaa aaggtgtaag gagtaaccga tgacaaccca gtatggattt tttattgatt
    25621 ccagccgttg caccggttgc aaaacctgcg agctggcttg taaagactac aaagatttga
    25681 cgccagaagt cagcttccgc cgcatttatg aatatgctgg cggcgactgg caggaagata
    25741 acggtgtctg gcaccagaac gtgtttgcct actatctgtc gatttcgtgc aaccactgcg
    25801 aagatccggc ttgtactaaa gtctgcccga gcggtgcgat gcataaacgt gaagatggtt
    25861 ttgttgtggt cgatgaagat gtgtgcattg gctgccgcta ctgccatatg gcttgcccgt
    25921 atggcgcacc gcaatataac gaaacgaaag gccatatgac caaatgcgat ggttgttatg
    25981 accgtgttgc ggagggtaaa aagccgatct gtgttgaatc ttgtccgctg cgggcgctgg
    26041 atttcggccc tatcgacgag ctgcgtaaaa aacatggcga tctggcggcc gttgcgccgt
    26101 tgccgcgagc tcactttacc aaaccgaata ttgtgatcaa acccaatgcc aatagccgcc
    26161 cgaccgggga taccactggc tatctggcaa acccgaagga ggtgtgagat gggaagtgga
    26221 tggcatgaat ggccgctgat gatcttcacg gtcttcgggc aatgtgtggc gggtggtttt
    26281 atcgttctgg ctttggcgct gctcaaaggc gacctgcgag cagaagccca gcagcgtgtt
    26341 atcgcctgca tgtttggctt atgggtgctg atgggcattg gctttatcgc ttctatgctc
    26401 catcttggtt caccgatgcg cgcttttaac tcgctcaacc gggtaggggc ttcagcactc
    26461 agtaacgaaa tcgccagcgg ttcgatcttt tttgccgtag gcggcatcgg ctggttgctg
    26521 gcaatgctga aaaagctgtc accggcattg cgtacgcttt ggctggtggt gacgatggtt
    26581 cttggcgtcg tctttgtctg gatgatggtg cgtgtgtata acagcattga taccgttccg
    26641 acctggtaca gcatctggac gccgatgggc ttcttcctga cgatgttcat gggcggcccg
    26701 ttactggggt acctgttgct gagtctggct ggcgtcgata gctgggcgat gcgtctgctg
    26761 ccagccattt ccgtactggc actggtggtg agtggcgtgg tgtcagtgat gcagggcgca
    26821 gagctggcga ctattcatag ttctgtgcag caggccgcag cgctggtgcc ggactatggt
    26881 gcgctgatgt cctggcggat cgtgcttttg gccgttgccc tgtgcttgtg gattgcacca
    26941 cagctaaaag gttatcagcc tgcggttccg ctactatccg tctcattcat tctgcttctg
    27001 gcaggggaat taatcggtcg cggcgtattc tatggtttgc atatgaccgt ggggatggcc
    27061 gtcgcaagct aatcagtaac aaccggggtt tcggccccgg ttctctttta tttctgcttc
    27121 gttaacgtgt cataactggt catcaaatta cgataatcag gaatatggtt agagaacaac
    27181 gtcgccagtc cttcaatatc attacgccag tcacggtgca gctcgcaagc cacgccgaac
    27241 catgtcatca attgcgcacc tgcttgcgac atgcgatccc atgccgaatg ccgggtaatt
    27301 tcattaaagg tccctgacgc gtcagtcaca acaaacacat caaacccttc ttcaatggct
    27361 gaaagcgccg ggaatgccac gcaaacttcg gttaccacac cggcaataat taactgtttt
    27421 ttacctgtcg ctttgactgc ttttacaaaa tcttcgttat cccaggcgtt aatattgccc
    27481 gggcgagcaa tgtaaggtgc atcggggaat tgtgctttca gttctggaac taatgggccg
    27541 ttaggaccgg tttcaaaact ggtggtaaga atagttggta agttgaaata cttagccagg
    27601 tcacccagcg ccagcacgtt atttttaaac ttatcgggtt cgatatcccg tacaagggaa
    27661 agtaaaccag cctggtgatc aacaagcaaa acagcagcat catttttatc aagacgaaca
    27721 tacggtttgg tcatcctctt ctcctttcga gagtcagtgg tacgcctgaa atcggtcaga
    27781 cgtgatgcat tgctctgaaa gcatagacgg gaaatatgag tttgctgtga ccatgaaatt
    27841 tttcgactga acgaagcgta tgaaatttgt gttagatcaa taaaaacaat cagatgagat
    27901 taatcatctc ttcagtatta gcgttatgaa tgagtaatat aaatcgataa taccgccgtt
    27961 gcgatatgcg cgtattttca cccattgaca atgtttttgg cggtggcatg atgcgcatga
    28021 aatttgaact tcctcacggt tttaattcat gtccacgtat acccggcctg tcatgctttt
    28081 gctgtctggc ctgcttttgt tgactctggc gatcgcggtg ttaaatacac tcgtgccgct
    28141 ttggctcgcc caggaacaca tgtccacatg gcaggtaggc gttgtcagct catcctattt
    28201 taccggcaac cttgtcggta cattgctgac agggtatgtc attaaacgca ttggttttaa
    28261 ccgcagctat tatctggcct ccttcatttt tgccgctggc tgtgccggcc ttggcctgat
    28321 gattggattc tggagttggt tggcgtggcg ttttgtcgcg ggcgtcggct gtgccatgat
    28381 ttgggtagtc gttgagagcg cgctgatgtg cagtgggacg tcacgtaacc gtgggcgttt
    28441 gcttgctgca tacatgatgg tttattacgt gggaacgttt ttaggccagt tactggtcag
    28501 caaggtctca accgagctga tgagcgtatt gccgtgggtt acaggtttga cattggcagg
    28561 gatcttaccg ctgttgttta cgcgtgtcct gaatcagcag gctgaaaacc atgattcgac
    28621 gtcaattacg gcaatgctaa aactccgtca ggcgcggctt ggcgtgaatg gctgcattat
    28681 ctcaggaatc gttctgggat ctctatatgg cctgatgcct ctgtacctca atcacaaagg
    28741 ggtgagcaat gccagtattg gtttctggat ggcggtactg gtcagtgcgg gtatcctcgg
    28801 acaatggccg attggacgtc tggcggataa gtttggtcga ctgctggttt tgcgtgttca
    28861 ggtctttgtc gtcattctcg gcagtattgc gatgcttagc caggcggcga tggccccagc
    28921 gttattcatc ctcggtgccg ctggctttac gctatatccg gtggcgatgg catgggcttg
    28981 cgagaaagtt gaacatcatc aactggtggc gatgaaccaa gccttactgt tgagctatac
    29041 tgtgggaagt ctgcttggcc cgtcatttac cgctatgcta atgcagaatt tctccgataa
    29101 tttattgttt atcatgatcg ccagcgtatc gtttatctat ttgctgatgc tgctgcgcaa
    29161 cgccggtcat acgccgaaac ccgttgctca cgtgtaaatg aattcaagca gagtgtgaac
    29221 ttactgtttc acactctgct tttttgtttc ttctatctga cttgctttat tccaaatttt
    29281 attcgtttaa aaataaaatg tgtagcaggt tataattttg catttcgcta tttccgcact
    29341 tcttatttgc cgcgcataat ccctcgtttt acagatgccc ctataatttt ggcgaaggat
    29401 ttgtctatgg ctgggaatgt tcaggaaaaa cagctgcgat ggtacaacat tgcgctgatg
    29461 tcttttatca ctgtctgggg ttttggcaac gttgttaata actacgccaa ccaggggctg
    29521 gtggttgttt tttcatgggt gtttatcttt gcgctctatt tcacacctta tgcgctaatt
    29581 gttggtcagt taggctcgac cttcaaagat gggaagggcg gggtcagtac ctggattaaa
    29641 cacacgatgg gacccggact ggcttatctc gccgcgtgga cctactgggt ggtgcatatt
    29701 ccctatctgg cacaaaaacc ctaggcaatt ctgattgcgc tcggttgggc gatgaaaggc
    29761 gacggttcgc taatcaaaga atattcagtc gtagcgttac aggggttaac gctggtgctg
    29821 tttatcttct ttatgtgggt tgcttcacgc ggtatgaaat cgctgaaaat cgtcggttct
    29881 gtggcaggga ttgctatgtt tgttatgtcg ctcctgtatg tggcgatggc ggtaaccgcg
    29941 cctgcaatta ctgaagtgca tattgcgacc acaaacatta cctgggaaac gttcattcct
    30001 catatcgact ttacctacat taccactatt tcaatgctgg ttttcgcggt tggcggagca
    30061 gagaagattt ctccttacgt taatcaaacg cgcaacccag gaaaagaatt tccaaaaggg
    30121 atgttatgcc tggcggtgat ggttgcggtt tgtgccattc tgggctcgct ggcgatgggg
    30181 atgatgtttg attcgcgtaa tatcccggat gacttaatga ccaacggtca gtattacgcc
    30241 tttcagaagc tgggcgagta ttacaacatg ggtaatactt taatggtgat ttacgccatt
    30301 gcgaataccc tgggacaagt agcggcgctg gtattctcga ttgatgcccc gcttaaagtg
    30361 ctattaggtg atgctgacag caaatatatt ccagccagtt tatgtcgtac caacgcttct
    30421 ggtacgcccg ttaatggcta ttttctgacc ctggtactgg tggcgattct gattatgctg
    30481 ccgactctcg gcattggtga tatgaacaat ctctataaat ggctgttgaa ccttaattcg
    30541 gtagtgatgc cgctgcgtta tttatgggta tttgttgcat ttattgcagt cgttcgcctg
    30601 gcgcagaaat ataaaccaga atatgtcttt attcgtaata agcctctggc aatgaccgtc
    30661 gggatttggt gttttgcctt taccgccttt gcctgtttga cggggatctt cccgaaaatg
    30721 gaagccttca ctgcagagtg gaccttccag ttggcgctga atgttgcaac gccgtttgtg
    30781 ctggtaggat taggactgat attcccgctg ctggcgcgta aagcgaatag taaataatta
    30841 ttgtggtcgg tgacgctgac tctggcagat ctgacccgtt tttacataat cgagaaatgc
    30901 gcgcaagccg caggacatat actggcggtt tgggtaatag atctggaagc ctggacgctc
    30961 tgtgatccag tcttccagta cacattctaa acgaccggta tcaaggtact ccttgatctg
    31021 ttcatagagc agatacccga taccagcacc catcaaaacg gcatccagtt cagcatcaac
    31081 atcat
//
LOCUS       DADMKF010000035        30188 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.036, whole genome shotgun sequence.
ACCESSION   DADMKF010000035 DADMKF010000000
VERSION     DADMKF010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30188
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.036"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            <1..234
                     /locus_tag="JNC24_001455"
     CDS             <1..234
                     /locus_tag="JNC24_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAY9158232.1"
                     /translation="GRHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKMVV
                     TLIHPIAMDDGLRFAIREGGRTVGAGVVAKVLS"
     gene            464..847
                     /gene="secE"
                     /locus_tag="JNC24_001456"
     CDS             464..847
                     /gene="secE"
                     /locus_tag="JNC24_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HAY9158233.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLVAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            849..1394
                     /gene="nusG"
                     /locus_tag="JNC24_001457"
     CDS             849..1394
                     /gene="nusG"
                     /locus_tag="JNC24_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HAY9158234.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1553..1981
                     /gene="rplK"
                     /locus_tag="JNC24_001458"
     CDS             1553..1981
                     /gene="rplK"
                     /locus_tag="JNC24_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HAY9158235.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            1985..2689
                     /gene="rplA"
                     /locus_tag="JNC24_001459"
     CDS             1985..2689
                     /gene="rplA"
                     /locus_tag="JNC24_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HAY9158236.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            3102..3599
                     /gene="rplJ"
                     /locus_tag="JNC24_001460"
     CDS             3102..3599
                     /gene="rplJ"
                     /locus_tag="JNC24_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HAY9158237.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3666..4031
                     /gene="rplL"
                     /locus_tag="JNC24_001461"
     CDS             3666..4031
                     /gene="rplL"
                     /locus_tag="JNC24_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HAY9158238.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKSLEEAGAEVEVK"
     gene            4351..8379
                     /gene="rpoB"
                     /locus_tag="JNC24_001462"
     CDS             4351..8379
                     /gene="rpoB"
                     /locus_tag="JNC24_001462"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAY9158239.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8456..12679
                     /gene="rpoC"
                     /locus_tag="JNC24_001463"
     CDS             8456..12679
                     /gene="rpoC"
                     /locus_tag="JNC24_001463"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HAY9158240.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            complement(12921..14054)
                     /gene="thiH"
                     /locus_tag="JNC24_001464"
     CDS             complement(12921..14054)
                     /gene="thiH"
                     /locus_tag="JNC24_001464"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HAY9158241.1"
                     /translation="MKTFSDRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEPLAQRAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYTRHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAALTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(14051..14896)
                     /gene="thiG"
                     /locus_tag="JNC24_001465"
     CDS             complement(14051..14896)
                     /gene="thiG"
                     /locus_tag="JNC24_001465"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709785.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HAY9158242.1"
                     /translation="MSSGRNISCRMATRSCFFRLLQGVEMLRIADKTFDSHLFTGTGK
                     FASSQLMVEAIRASGSQLVTLAMKRVDLRQHNDAILEPLIAAGVTLLPNTSGAKTAEE
                     AIFAAHLAREALGTNWLKLEIHPDARWLLPDPIETLKAAEMLVQQGFVVLPYCGADPV
                     LCKRLEEVGCAAVMPLGAPIGSNQGLETRAMLEIIIQQATVPVVVDAGIGVPSHAAQA
                     LEMGADAVLVNTAIAVADDPVNMAKAFRLAVEAGLLARQSGPGSRSHFAHATSPLTGF
                     LEASA"
     gene            complement(14823..15023)
                     /gene="thiS"
                     /locus_tag="JNC24_001466"
     CDS             complement(14823..15023)
                     /gene="thiS"
                     /locus_tag="JNC24_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HAY9158243.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWTQHIVQDGDQILLFQVIAGG"
     gene            complement(15007..15762)
                     /gene="thiF"
                     /locus_tag="JNC24_001467"
     CDS             complement(15007..15762)
                     /gene="thiF"
                     /locus_tag="JNC24_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HAY9158244.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDIDRPKSQVSHQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVARADVVLDCTDNMATRQEINAACVVLNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQEPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(15755..16390)
                     /gene="thiE"
                     /locus_tag="JNC24_001468"
     CDS             complement(15755..16390)
                     /gene="thiE"
                     /locus_tag="JNC24_001468"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HAY9158245.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     NEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(16390..18285)
                     /gene="thiC"
                     /locus_tag="JNC24_001469"
     CDS             complement(16390..18285)
                     /gene="thiC"
                     /locus_tag="JNC24_001469"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HAY9158246.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARGDTEELTVRSSDYTRTRLADDGLDELRFSGLLTPKRAKTGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGAHLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAVHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAAAQTIEVGMADMSENFRARGGEI
                     YLRKEEA"
     regulatory      complement(18366..18466)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(18519..18995)
                     /gene="rsd"
                     /locus_tag="JNC24_001470"
     CDS             complement(18519..18995)
                     /gene="rsd"
                     /locus_tag="JNC24_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HAY9158247.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            19090..19863
                     /gene="nudC"
                     /locus_tag="JNC24_001471"
     CDS             19090..19863
                     /gene="nudC"
                     /locus_tag="JNC24_001471"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HAY9158248.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLVQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            19903..20967
                     /gene="hemE"
                     /locus_tag="JNC24_001472"
     CDS             19903..20967
                     /gene="hemE"
                     /locus_tag="JNC24_001472"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HAY9158249.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRRELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            20977..21648
                     /gene="nfi"
                     /locus_tag="JNC24_001473"
     CDS             20977..21648
                     /gene="nfi"
                     /locus_tag="JNC24_001473"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HAY9158250.1"
                     /translation="MDLASLRAQQIELASSVIREDRLDKDPPDLIAGVDVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLMVDVPTIGVAKKRLCGKFEPLSSEPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            21691..22281
                     /locus_tag="JNC24_001474"
     CDS             21691..22281
                     /locus_tag="JNC24_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HAY9158251.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLREAGESNIGIIFQQ"
     gene            22468..22740
                     /gene="hupA"
                     /locus_tag="JNC24_001475"
     CDS             22468..22740
                     /gene="hupA"
                     /locus_tag="JNC24_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-alpha"
                     /protein_id="HAY9158252.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            22753..23448
                     /locus_tag="JNC24_001476"
     CDS             22753..23448
                     /locus_tag="JNC24_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HAY9158253.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALLLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(23450..23875)
                     /gene="zraP"
                     /locus_tag="JNC24_001477"
     CDS             complement(23450..23875)
                     /gene="zraP"
                     /locus_tag="JNC24_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HAY9158254.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            24113..25489
                     /gene="zraS"
                     /locus_tag="JNC24_001478"
     CDS             24113..25489
                     /gene="zraS"
                     /locus_tag="JNC24_001478"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HAY9158255.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMHR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGAGVKISVTDSGKG
                     IAADQLDAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGATFTLWLPVT
                     ITRKGPQG"
     gene            25486..26811
                     /gene="zraR"
                     /locus_tag="JNC24_001479"
     CDS             25486..26811
                     /gene="zraR"
                     /locus_tag="JNC24_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HAY9158256.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGALD
                     YLIKPLDFDNLQATLEKALAHTHSIDAETPAVSASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADRRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAVHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIAGTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(26808..28097)
                     /gene="purD"
                     /locus_tag="JNC24_001480"
     CDS             complement(26808..28097)
                     /gene="purD"
                     /locus_tag="JNC24_001480"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HAY9158257.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGMKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALAYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDTGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLGV
                     VMAAGGYPGDYRTGDVIHGLPLEEVAGGKVFHAGTKLADNEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(28109..29698)
                     /gene="purH"
                     /locus_tag="JNC24_001481"
     CDS             complement(28109..29698)
                     /gene="purH"
                     /locus_tag="JNC24_001481"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HAY9158258.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7066 a         7873 c         8313 g         6936 t
ORIGIN      
        1 ggtcgtcata ctccgttctt caaaggctac cgtccgcagt tctacttccg tactactgac
       61 gtgactggta ccatcgaact gccggaaggc gtagagatgg taatgccggg cgacaacatc
      121 aaaatggttg ttaccctgat ccacccgatc gcgatggacg acggtctgcg tttcgcaatc
      181 cgtgaaggcg gccgtaccgt tggcgcgggc gttgtagcaa aagttctgag ctaattgccg
      241 ataacatttg acgcaatgcg cactaaaagg gcatcatttg atgccctttt tgcacgcttt
      301 cgtaccagaa cctggctcat cagtgatttt ctttgtcata atcattgctg agacaggctc
      361 tgttgagggc gtataatccg aaaagctaat acgcgtttcg atttggtttg cctcgcgatc
      421 gcggggtgaa aatgtttgta gaaaacttct gacaggttgg tttatgagtg cgaataccga
      481 agctcaagga agcgggcgcg gcctggaagc gatgaagtgg gtcgttgtgg tggcattgct
      541 cctggtggcg attgtcggca actaccttta tcgcgacatt atgctgccgc tgcgtgcgct
      601 ggccgtagta attctgattg ctgcagcggg tggtgtcgcg ctgttaacga caaaaggtaa
      661 agctaccgtt gcttttgccc gtgaagcgcg taccgaagtc cgtaaggtca tttggccgac
      721 tcgccaggaa acattgcaca ccacgctgat tgtggctgcg gttaccgcag taatgtcact
      781 gatcctgtgg ggactggatg gtattctggt tcgcctggta tcctttatca ctggcctgag
      841 gttctgagat gtctgaagct cctaaaaagc gctggtacgt cgttcaggcg ttttccggtt
      901 ttgaaggccg cgtagcaacg tcgctacgtg agcatatcaa attacacaac atggaagatt
      961 tgtttggtga agtcatggta ccaaccgaag aagtggttga aatccgtggc ggtcagcgtc
     1021 gcaaaagcga acgtaaattc ttccctggct acgtcctcgt tcagatggtg atgaacgacg
     1081 cgagctggca cctggtgcgc agcgtaccgc gtgtgatggg cttcatcggc ggtacttccg
     1141 atcgtcctgc gccaatcagc gataaagaag tcgatgcgat tatgaaccgc ctgcagcagg
     1201 ttggtgataa gccgcgtccg aaaacgctgt ttgaaccggg tgaaatggtc cgtgttaatg
     1261 atggtccgtt cgctgacttc aacggtgttg ttgaagaagt ggattacgag aaatctcgtc
     1321 tgaaagtgtc tgtttctatc ttcggtcgtg cgaccccggt agagctggac ttcagccagg
     1381 ttgaaaaagc ctaacccagc gatcaaaaaa gcggcgattt aatcgttgca caaggcgtga
     1441 gattggaata caatttcgcg ccttttgttt ttatgggcct tgcccgtaaa acgatttttt
     1501 atatcacggg gagcctctca gaggcgttat tacccaactt gaggaattta taatggctaa
     1561 gaaagtacaa gcctatgtca agctgcaggt tgcagctggt atggctaacc cgagtccgcc
     1621 agtaggtccg gctctgggtc agcagggcgt aaacatcatg gaattctgca aagcgttcaa
     1681 cgcaaaaact gattccatcg aaaaaggtct gccgattccg gtagtaatca ccgtttacgc
     1741 tgaccgttct ttcactttcg ttaccaagac tccgccggca gcagttctgc tgaaaaaagc
     1801 ggctggtatc aagtctggtt ccggtaagcc gaacaaagac aaagtgggta aaatttcccg
     1861 cgctcagctg caggaaatcg cgcagaccaa agctgccgac atgactggtg ccgacattga
     1921 agcgatgact cgctccatcg aaggtactgc acgttccatg ggcctggtag tggaggacta
     1981 agaaatggct aaactgacca agcgcatgcg tgttatccgc gagaaagttg atgcaaccaa
     2041 acagtacgac atcaacgaag ctatcgcact gctgaaagag ctggcgactg ctaaattcgt
     2101 agaaagcgtg gacgtagctg ttaacctcgg catcgacgct cgtaaatctg accagaacgt
     2161 acgtggtgca actgtactgc cgcacggtac tggccgttcc gttcgcgtag ccgtatttac
     2221 ccaaggtgca aacgctgaag ctgctaaagc tgcaggcgca gaactggtag gtatggaaga
     2281 tctggctgac cagatcaaga aaggcgaaat gaactttgac gttgttattg cttctccgga
     2341 tgcaatgcgc gttgttggcc agctgggcca ggttctgggt ccgcgcggcc tgatgccaaa
     2401 cccgaaagtg ggtactgtaa cgccgaacgt tgctgaagcg gttaaaaacg ctaaagctgg
     2461 ccaggttcgt taccgtaacg acaaaaacgg catcatccac accaccatcg gtaaagtgga
     2521 ctttgacgct gacaaactga aagaaaacct ggaagctctg ctggttgcgc tgaaaaaagc
     2581 aaaaccgact caggcgaaag gcgtgtacat caagaaagtt agcatctcca ccaccatggg
     2641 tgcaggtgtt gcagttgacc aggctggcct gagcgcttct gtaaactaat gcctttacgt
     2701 gggcggtgat tttgtctaca atcttacccc cacatataat gcttaatgca gacgtatatc
     2761 cgagatattc gggttgtggc aaggcggcaa ctgagtgagt cgccaggagc atagctaact
     2821 atgtgactgg tgcgaatgaa ggaagccaac gccgccacaa gttgaatagc gacggataga
     2881 aaagatttgt tcgttggagc ctggcctatc caggcctccg tcgaagaccg caggagtttc
     2941 gcaagaaact taatcccctg cgtagacggt gacagaacgc taagattatt cttttatatt
     3001 ctggcttgtt tctgctcacc gtaattaaga cgctctctcc gcttggagga gtgaagtgag
     3061 ttccagagat tttctctggc aaacatccag gagcaaagct aatggcttta aatcttcaag
     3121 acaaacaagc gattgttgct gaagtcagcg aagtagccaa aggcgcgctg tctgcagtag
     3181 ttgcggattc ccgtggcgta actgtagata aaatgactga actgcgtaaa gcaggtcgcg
     3241 aagctggcgt atacatgcgt gttgttcgta acaccctgct gcgccgtgct gttgaaggta
     3301 ctccgttcga gtgcctgaaa gacgcgtttg ttggtccgac cctgattgca tactctatgg
     3361 aacacccggg cgctgctgct cgtctgttca aagagttcgc gaaagcgaat gcaaaatttg
     3421 aggtcaaagc cgctgccttt gaaggtgagc tgatcccggc gtctcagatc gaccgcctgg
     3481 caactctgcc gacctacgaa gaagcaattg cacgcctgat ggcaaccatg aaagaagctt
     3541 cggctggtaa actggttcgt actctggctg ctgtacgcga tgcgaaagaa gctgcttaat
     3601 cgcagttatc tttttaacgc attcgcttac gtataaactt attctgatat tcaggaacaa
     3661 tttaaatgtc tatcactaaa gatcaaatca ttgaagcagt tgcagctatg tctgtaatgg
     3721 acgttgtaga actgatctct gcaatggaag aaaaattcgg tgtttccgct gctgctgctg
     3781 tagctgtagc tgctggcccg gttgaagctg ctgaagaaaa aactgaattc gacgtaattc
     3841 tgaaagctgc tggcgctaac aaagttgctg ttatcaaagc agtacgtggc gcaactggcc
     3901 tgggtctgaa agaagctaaa gacctggtag aatctgcacc ggctgctctg aaagaaggcg
     3961 tgagcaaaga cgacgcagaa gcactgaaaa aatccctgga agaagctggc gctgaagttg
     4021 aagttaaata agccaaccct tccggttgca gcctgagaaa tcaggctgat ggctggtgac
     4081 tttttggtca ccagcctttt tgcgctgtaa ggcgccagta gcgtttcaca ctgtttgact
     4141 actgctgtgc ctttcaatgc ttgtttctat cgacgactta atatactgcg acaggacgtc
     4201 cgttctgtgt aaatcgcaat gaaatggttt aagcgtgata gcaacaggca ttgcggaaag
     4261 tgttccattt tccggtcaac aaaatagtgt tgcacaaact gtccgctcaa tggacagatg
     4321 ggtcgacttg tcagcgagct gaggaaccct atggtttact cctataccga gaaaaaacgt
     4381 attcgtaagg attttggtaa acgtccacaa gttctggatg taccttatct cctttctatc
     4441 cagcttgact cgtttcagaa atttatcgag caagatcctg aagggcagta tggtctggaa
     4501 gctgctttcc gttccgtatt cccgattcag agctacagcg gtaattccga gctgcaatac
     4561 gtcagctacc gccttggcga accggtgttt gacgtccagg aatgtcaaat ccgtggcgtg
     4621 acctattccg caccgctgcg cgttaaactg cgtctggtga tctatgagcg cgaagcgccg
     4681 gaaggcaccg taaaagacat taaagaacaa gaagtctaca tgggcgaaat tccgctcatg
     4741 acagacaacg gtacctttgt tatcaacggt actgagcgtg ttatcgtttc ccagctgcac
     4801 cgtagtccgg gcgtcttctt tgactccgac aaaggtaaaa cccactcttc gggtaaagtg
     4861 ctgtataacg cgcgcatcat cccttaccgt ggttcctggc tggacttcga attcgatccg
     4921 aaggacaacc tgttcgtacg tatcgaccgt cgccgtaaac tgcctgcgac catcattctg
     4981 cgcgccctga actacaccac agagcagatc ctcgacctgt tctttgaaaa agttatcttt
     5041 gaaatccgtg ataacaagct gcagatggag ctggtgccgg aacgcctgcg tggtgaaact
     5101 gcatctttcg acatcgaagc taacggtaaa gtgtacgtag aaaaaggccg ccgtatcact
     5161 gcgcgccaca ttcgccagct ggaaaaagac gacgtcaaac tgatcgaagt cccggttgag
     5221 tacatcgcag gtaaagtggt tgctaaagac tatattgatg agtctaccgg cgagctgatc
     5281 tgcgcagcga acatggagct gagcctggat ctgttggcta agctgagcca gtctggtcac
     5341 aagcgtattg aaacgctgtt caccaacgac ctggatcacg gcccgtatat ctctgaaacc
     5401 ttacgtgtcg acccaactaa cgaccgtctg agcgcactgg tagaaatcta ccgcatgatg
     5461 cgccctggcg agccgccgac tcgtgaagca gctgaaagcc tgttcgagaa cctgttcttc
     5521 tccgaagacc gttatgacct gtctgcggtt ggtcgtatga agttcaaccg ttctctgctg
     5581 cgcgaagaaa tcgaaggttc cggtatcctg agcaaagacg acatcattga tgttatgaaa
     5641 aagctcatcg atatccgtaa cggtaaaggc gaagtcgatg atatcgacca cctcggcaac
     5701 cgtcgtatcc gttctgttgg cgaaatggcg gaaaaccagt tccgcgttgg cctggtacgt
     5761 gtagagcgtg cggtgaaaga gcgtctgtct ctgggcgatc tggataccct gatgcctcag
     5821 gacatgatca acgccaagcc gatttccgca gcagtgaaag agttcttcgg ttccagccag
     5881 ctgtctcagt ttatggacca gaacaacccg ctgtctgaga ttacgcacaa acgtcgtatc
     5941 tccgcactcg gcccgggtgg tctgacccgt gaacgtgcag gcttcgaagt tcgagacgta
     6001 cacccgactc attacggtcg cgtatgtcca atcgaaaccc ctgaaggtcc gaacatcggt
     6061 ctgatcaact ctttgtccgt gtacgcacag actaacgaat acggcttcct tgagactccg
     6121 tatcgtaagg tgaccgacgg tgttgtaact gacgaaattc actacctttc tgctatcgaa
     6181 gaaggcaact acgttatcgc ccaggcgaac tccaacctgg atgaagaagg ccacttcgta
     6241 gaagacctgg taacttgccg tagcaaaggc gaatccagct tgttcagccg tgaccaggtt
     6301 gactacatgg acgtatccac ccagcaggtg gtatccgtcg gtgcgtccct gatcccgttc
     6361 ctggaacacg atgacgccaa ccgtgcattg atgggtgcga acatgcaacg tcaggccgtt
     6421 ccgactctgc gcgctgataa gccgctggtt ggtactggta tggaacgtgc tgttgccgtt
     6481 gactccggtg taactgcggt agctaaacgt ggtggtgtcg ttcagtacgt ggatgcttcc
     6541 cgtatcgtta tcaaagttaa cgaagacgag atgtatccgg gtgaagcagg tatcgacatc
     6601 tacaacctga ccaaatacac ccgttctaac cagaacacct gtatcaacca gatgccgtgt
     6661 gtgtctctgg gtgaaccggt tgaacgtggc gacgtgctgg cagacggtcc gtccaccgac
     6721 ctcggtgaac tggcgcttgg tcagaacatg cgcgtagcgt tcatgccgtg gaatggttac
     6781 aacttcgaag actccatcct cgtatccgag cgtgttgttc aggaagaccg tttcaccacc
     6841 atccacattc aggaactggc gtgtgtgtcc cgtgacacca agctggggcc ggaagagatc
     6901 accgctgaca tcccgaacgt gggtgaagct gcgctctcca aactggatga atccggtatc
     6961 gtttacattg gtgcggaagt gaccggtggc gacattctgg ttggtaaggt aacgccgaaa
     7021 ggtgaaactc agctgacccc agaagaaaaa ctgctgcgtg cgatcttcgg tgagaaagcc
     7081 tctgacgtta aagactcttc tctgcgcgta ccaaacggtg tatccggtac ggttatcgac
     7141 gttcaggtct ttactcgcga tggcgtagaa aaagacaaac gtgcgctgga aatcgaagaa
     7201 atgcagctca aacaggcgaa gaaagacctg tctgaagaac tgcagatcct cgaagcgggt
     7261 ttgttcagcc gtatccgtgc tgtgctggta gccggtggcg ttgaagctga gaagctcgac
     7321 aaactgccgc gcgatcgctg gctggagctg ggcctgaccg acgaagagaa acaaaatcag
     7381 ctggaacagc tggctgagca gtatgacgaa ctgaaacacg agttcgagaa gaaactcgaa
     7441 gcgaaacgcc gcaaaatcac ccagggcgac gatctggcac cgggcgtgct gaagattgtt
     7501 aaggtgtatc tggcggttaa acgccgtatc cagcctggtg acaagatggc aggtcgtcac
     7561 ggtaacaagg gtgtaatttc taagatcaac ccgatcgaag atatgcctta cgatgaaaac
     7621 ggtacgccgg tagacatcgt actgaacccg ctgggcgtac cgtctcgtat gaacatcggt
     7681 cagatcctcg aaacccacct gggtatggct gcgaaaggta tcggcgacaa gatcaacgcc
     7741 atgctgaaac agcagcaaga agtcgcgaaa ctgcgcgaat tcatccagcg tgcgtacgat
     7801 ctgggcgctg acgttcgtca gaaagttgac ctgagtacct tcagcgatga agaagttatg
     7861 cgtctggctg aaaacctgcg caaaggtatg ccaatcgcaa cgccggtgtt cgacggtgcg
     7921 aaagaagcag aaattaaaga gctgctgaaa cttggcgacc tgccgacttc cggtcagatc
     7981 cgcctgtacg atggtcgcac tggtgaacag ttcgagcgtc cggtaaccgt tggttacatg
     8041 tacatgctga aactgaacca cctggtcgac gacaagatgc acgcgcgttc caccggttct
     8101 tacagcctgg ttactcagca gccgctgggt ggtaaggcac agttcggtgg tcagcgtttc
     8161 ggggagatgg aagtgtgggc gctggaagca tacggcgcag catacaccct gcaggaaatg
     8221 ctcaccgtta agtctgatga cgtgaacggt cgtaccaaga tgtataaaaa catcgtggac
     8281 ggcaaccatc agatggagcc gggcatgcca gaatccttca acgtattgtt gaaagagatt
     8341 cgttcgctgg gtatcaacat cgaactggaa gacgagtaat tctcgctcaa acaggtcact
     8401 gctgtcgggg taaaacccgg cagcggattg tgctaactcc gacgggagca aatccgtgaa
     8461 agatttatta aagtttctga aagcgcagac taaaaccgaa gagtttgatg cgatcaaaat
     8521 tgctctggct tcgccagaca tgatccgttc atggtctttc ggtgaagtta aaaagccgga
     8581 aaccatcaac taccgtacgt tcaaaccaga acgtgacggc cttttctgcg cccgtatctt
     8641 tgggccggta aaagattacg agtgcctgtg cggtaagtac aagcgcctga aacaccgtgg
     8701 cgtcatctgt gagaagtgcg gcgttgaagt gacccagact aaagtacgcc gtgagcgtat
     8761 gggccacatc gaactggctt ccccgactgc gcacatctgg ttcctgaaat cgctgccgtc
     8821 ccgtatcggt ctgctgctcg atatgccgct gcgcgatatc gaacgcgtac tgtactttga
     8881 atcctatgtg gttatcgaag gcggtatgac caacctggaa cgtcagcaga tcctgactga
     8941 agagcagtat ctggacgcgc tggaagagtt cggtgacgaa ttcgacgcga agatgggtgc
     9001 ggaagcaatc caggctctgc tgaagagcat ggatctggag caagagtgcg aacagctgcg
     9061 tgaagagctg aacgaaacca actccgaaac caagcgtaaa aagctgacca agcgtatcaa
     9121 actgctggaa gcgttcgttc agtctggtaa caaaccagag tggatgatcc tgaccgttct
     9181 gccggtactg ccgccagatc tgcgtccact ggttccgctg gatggtggtc gtttcgcgac
     9241 ttctgacctg aacgatctgt atcgtcgcgt cattaaccgt aacaaccgtc tgaaacgtct
     9301 gctggatctg gctgcgccgg acatcatcgt acgtaacgaa aaacgtatgc tgcaggaagc
     9361 ggtagacgcc ctgctggata acggtcgtcg cggtcgtgcg atcaccggtt ctaacaagcg
     9421 tcctctgaaa tctttggccg acatgatcaa aggtaaacag ggtcgtttcc gtcagaacct
     9481 gctcggtaag cgtgttgact actccggtcg ttctgtaatc accgtaggtc catacctgcg
     9541 tctgcatcag tgcggtctgc cgaagaaaat ggcgctggag ctgttcaaac cgttcatcta
     9601 cggcaagctg gaactgcgtg gtcttgctac caccattaaa gctgcgaaga aaatggttga
     9661 gcgcgaagaa gctgtcgttt gggatatcct ggacgaagtt atccgcgaac acccggtact
     9721 gctgaaccgt gcaccgactc tgcaccgtct gggtatccag gcatttgaac cggtactgat
     9781 cgaaggtaaa gctatccagc tgcacccgct ggtttgtgcg gcatataacg ccgacttcga
     9841 tggtgaccag atggctgttc acgtaccgct gacgctggaa gcccagctgg aagcgcgtgc
     9901 gctgatgatg tctaccaaca acatcctgtc cccggcgaac ggcgaaccaa tcatcgttcc
     9961 gtctcaggac gttgttctgg gtctgtacta tatgacccgt gactgtgtta acgccaaagg
    10021 cgaaggcatg gtgctgactg gcccgaaaga agcagaacgt ctgtatcgct ctggtctggc
    10081 ttctctgcat gcgcgcgtta aagtgcgtat caccgagtat gaaaaagatg caaacggtga
    10141 attagtagcg aaaaccagcc tgaaagacac gactgttggc cgtgccattc tgtggatgat
    10201 tgtaccgaaa ggtctgcctt actccatcgt caaccaggcg ctgggtaaaa aagcaatctc
    10261 caaaatgctg aacacctgct accgcattct cggtctgaaa ccgaccgtta tttttgcgga
    10321 ccagatcatg tacaccggct tcgcctatgc agcgcgttct ggtgcatctg ttggtatcga
    10381 tgacatggtc atcccggaga agaaacacga aatcatctcc gaggcagaag cagaagttgc
    10441 tgaaattcag gagcagttcc agtctggtct ggtaactgcg ggcgaacgct acaacaaagt
    10501 tatcgatatc tgggctgcgg cgaacgatcg tgtatccaaa gcgatgatgg ataacctgca
    10561 aactgaaacc gtgattaacc gtgacggtca ggaagagaag caggtttcct tcaacagcat
    10621 ctacatgatg gccgactccg gtgcgcgtgg ttctgcggca cagattcgtc agcttgctgg
    10681 tatgcgtggt ctgatggcga agccggatgg ctccatcatc gaaacgccaa tcaccgcgaa
    10741 cttccgtgaa ggtctgaacg tactccagta cttcatctcc acccacggtg ctcgtaaagg
    10801 tctggcggat accgcactga aaactgcgaa ctccggttac ctgactcgtc gtctggttga
    10861 cgtggcgcag gacctggtgg ttaccgaaga cgattgtggt acccatgaag gtatcatgat
    10921 gactccggtt atcgagggtg gtgacgttaa agagccgctg cgcgatcgcg tactgggtcg
    10981 tgtaactgct gaagacgttc tgaagccggg tactgctgat atcctcgttc cgcgcaacac
    11041 gctgctgcac gaacagtggt gtgacctgct ggaagagaac tctgtcgacg cggtaaaagt
    11101 acgttctgtt gtatcttgtg acaccgactt tggtgtatgt gcgcactgct acggtcgtga
    11161 cctggcgcgt ggccacatca tcaacaaagg tgaagcaatc ggtgttatcg cggcacagtc
    11221 catcggtgaa ccgggtacac agctgaccat gcgtacgttc cacatcggtg gtgcggcatc
    11281 tcgtgcggct gctgaatcca gcatccaggt gaaaaacaaa ggtagcatca agctcagcaa
    11341 cgtgaagtcg gttgtgaact ccagcggtaa actggttatc acttcccgta acaccgaact
    11401 gaaactgatc gacgaattcg gtcgtaccaa agaaagctac aaagtgcctt acggtgcggt
    11461 actggcgaaa ggcgatggcg aacaggttgc tggcggcgaa accgttgcaa actgggaccc
    11521 gcacaccatg ccggttatca ccgaagtaag cggttttgta cgctttactg acatgatcga
    11581 cggccagacc attactcgtc agaccgacga actgaccggt ctgtcttcgc tggtggttct
    11641 ggattccgca gaacgtaccg caggtggtaa agatctgcgt ccggcactga aaatcgttga
    11701 tgctcagggt aacgacgttc tgatcccagg taccgatatg cctgcgcagt acttcctgcc
    11761 gggtaaagcg attgttcagc tggaagatgg cgtacagatc agctctggtg acaccctggc
    11821 gcgtattccg caggaatccg gcggtaccaa ggacatcacc ggtggtctgc cgcgcgttgc
    11881 ggacctgttc gaagctcgtc gtccgaaaga gccggcaatc ctggctgaaa tcagcggtat
    11941 cgtttccttc ggtaaagaaa ccaaaggtaa acgtcgtctg gttatcaccc cggtagacgg
    12001 tagcgatccg tacgaagaga tgattccgaa atggcgtcag ctcaacgtgt tcgaaggtga
    12061 acgtgtagaa cgtggtgacg taatttccga cggtccggaa gcgccgcacg acattctgcg
    12121 tctgcgtggt gttcatgctg tgactcgtta catcgttaac gaagtacagg acgtataccg
    12181 tctgcagggc gttaagatta acgataaaca catcgaagtt atcgttcgtc agatgctgcg
    12241 taaagctacc atcgttaacg cgggcagctc cgacttcctg gaaggcgaac aggttgaata
    12301 ctctcgcgtc aagatcgcaa accgcgaact ggaagcgaac ggcaaagtgg gtgcaactta
    12361 ctcccgcgat ctgctgggta tcaccaaagc gtctctggca accgagtcct tcatctccgc
    12421 ggcatcgttc caggagacca ctcgcgtgct gaccgaagca gccgttgcgg gcaaacgcga
    12481 cgaactgcgc ggcctgaaag agaacgttat cgtgggtcgt ctgatcccgg caggtaccgg
    12541 ttacgcgtac caccaggatc gtatgcgtcg ccgtgctgcg ggtgaagctc cggctgcacc
    12601 gcaggtgact gcagaagacg catctgccag cctggcagaa ctgctgaacg caggtctggg
    12661 cggttctgat aacgagtaat cgttaatccg caaataacgt aaaaacccgc ttcggcgggt
    12721 ttttttatgg gtgttattca gagggcaaat tcttcacgga aaatattaag aaaggcaggg
    12781 cgaaacgagg aagaagcttt tcgccctgga caacattcct gctgacggca ctaccataaa
    12841 aaaacacaac gctttcaaca accttgcgtt tttcatcttc cgtagcggct cggaataaat
    12901 ttacgttctt acattccgtc tcatagtctt tgcgaggcgc gtcccagata gctgtcccag
    12961 tctttccata ccggctgcaa accctgagcg gttaacgcgg cagcaaccgc ttccggtctg
    13021 cgatcgtcgt gcggtgagaa ctgttccaac tcggggtgat tatcggcata gccacctggc
    13081 tgcgttttcg agaaggcgct gacgttattg atcgccagcg gaataacgcg atcgcgaaac
    13141 cacggtgatt cccgcgtgga gagtgacagt tcaatctccg gtgcaagcag tcggaaggcg
    13201 cagatggttt gcactaactg gcgttcatcc ataatcgacg caggctcaat gccgccagta
    13261 cacgggcgca ggcgcggaaa ggagacagag taacggcttt gccagtaatg ctgttgcagc
    13321 catagcaaat gttcggcaac catatagcag tcaacgcgcc agttgtcgga aaggccaatt
    13381 agcgcgccga ggcctatctt atcaatcccc gcacgcccca gccgatccgg cgtttccagc
    13441 cgccagaaga agtcctgttt tttgcctttc aaatgatggc gggtataagt cgcctcgtga
    13501 tatgtctcct gataaaccat caccccatcc agaccaagtt gctttaactc ggcgtattcc
    13561 gtctccgcca gcggttgcac ttccatttgt agtgaagaga actgttcacg cagggcgggg
    13621 agatgacgac gaaagtaatc catccccact ttcgcctgat gttcaccagt gactaatagc
    13681 agatgttcaa agcccatctc ccgtatagcg gcgctttccc tggcaatatc cgcttcatcc
    13741 agcgttttgc gcttgatgcg attactcatg gaaaatccgc agtacgtgca gtcgttagcg
    13801 caaagattgg aaagataaag cgggacgtag aaactaactg tgttgccaaa tcgctgacgg
    13861 gtcagacgct gcgcccgttg ggccagtggt tccagatagc cactggcggc tggcgataac
    13921 agcgccatca tatcgtcgcg ggtgagttgc gaggcattta gcgcccgttc tacgtcagca
    13981 gccgttttgc cgttgatacg caggcggatg tcgtcccagt ccagttgtcg ccagcgatcg
    14041 ctgaaggttt tcatgccgat gcctccagaa atccagtcag cgggctggtg gcatgagcaa
    14101 aatgactgcg gctgcccggt ccggactgac gtgccagcag gcctgcttct accgccagac
    14161 gaaatgcctt cgccatgttg acgggatcgt ccgctacggc aatcgccgta ttcactaaca
    14221 ccgcgtcggc ccccatttcc agggcctgcg cggcatggct gggaacgccg atgccagcat
    14281 cgacaaccac cggaactgtg gcctgctgga taataatctc cagcatggca cgggtttcca
    14341 gtccctgatt cgagccaatc ggcgcgccga gcggcatcac cgctgcacag ccgacttctt
    14401 ccagacgttt acacaatacc ggatcggccc cgcagtaagg cagcacgaca aatccctgtt
    14461 gtaccagcat ttcggcggct ttcagagttt cgatgggatc gggcaacagc cagcgggcgt
    14521 cagggtgaat ctctaatttt aaccagtttg tgcctaacgc ttcacgtgcc agatgggcgg
    14581 cgaaaatggc ttcttccgct gttttcgccc cggaagtatt tggcagcagg gtcacacccg
    14641 ccgcgataag cggttcgagg atagcgtcgt tgtgctggcg caagtcgaca cgtttcatcg
    14701 ccagtgtcac cagctggctg ccggaagcgc ggatcgcctc caccatcagt tgtgaagaag
    14761 cgaatttccc tgtgccggta aacagatgtg aatcaaacgt tttgtccgca atacgtaaca
    14821 tctcaacccc ctgcaataac ctgaaaaagc aggatctggt cgccatcctg cacgatatgt
    14881 tgcgtccact gctcacgcgg gacgatttgc tgattaatcg ccagagccgc gcccgcttgt
    14941 cgttggtcga gttgctccag tagttcgtga acagtttgcc cggcggcgca ctgcatcggt
    15001 tgatcgttaa acaggatctg cattgcttcc tccgcatacc gggcaaccac tggcgcggcg
    15061 caacgccagg ctgcgccact ggctcgattt accgtcgaac agtcggagtt ctcccgcagg
    15121 tgtctctata ccgcttaata acttaatggc ttccagtgcc tgcaaagtgc ccataacccc
    15181 gaccaccggg ccaaccacgc ccgccgtgcg gcagttgcgt tctggctcct ggttatctgg
    15241 ccacaggcag cggtaacacc cctgctccca gggcggcgtc agtaccatca actgaccgcc
    15301 aaatccgacc gcgctggcgg tgataagcgg cgtgttgagt accacgcagg cggcattaat
    15361 ctcctggcga gtcgccatat tgtcggtaca gtcgagcacc acatcggccc gtgcaaccgc
    15421 atcttttaac gcctcacccg ttaaccgttg ttgtaatgct gtcagttgaa tatcgggatt
    15481 caactgtgtc agtcgctgat ggctgacctg cgatttcggg cgatcgatat cttcagtggt
    15541 aaagaggatt tgtcgttgca gattgcttaa atgcacatcg tcgtcatctg ccagtaccag
    15601 cgtcccgacg ccagcccccg ccaggtatag cgcagcaggt gtacccagcc cgccaagacc
    15661 aataatcagc acctggctgt cgagcagttt ttgctgcccg tccagagcga tatcgtcgag
    15721 cagaatttgg cggctataac gcataaagtc acggtcattc atcgccaact cctgcaattt
    15781 ccagcaactg tgccgttgcc aaacgccagt ctgcggcttg agtaatggcg ctgacgacgg
    15841 cgatactgcc gacacccgtt gctatcaccg caggcgcgcg cgccagactg ataccgccaa
    15901 tcgccacggt gggataatcc gccagtcgct caacatgccg tgccagctgt tccagcccct
    15961 gcggtgcaga aggcatctgt ttggtttgcg tcgggaacac atgtcccagc gcgatataag
    16021 aggggcgtgc tgccagcgcg acgtcgattt ccatatcgtc atgtgtcgaa acgcccagcc
    16081 gcaggcctgc cgcgcggatg gcattgagat cggtggcttg caaatcttcc tgccccaaat
    16141 ggacgccata cgcctgatgc ttgatcgcca gccgccagta atcgttgata aacaatcgcg
    16201 cgttatagcg gcggcccagc gcaattgccg ccacgacatc ggcttccacc tcttcattgc
    16261 gccgatcttt gatgcgtagc tggagggtac gtacgcctgc atccaacaga cgttcgatcc
    16321 actgtacgct gtccaccacc gggtacagtc ctaaacgaaa aggtacagga ggaaaatcag
    16381 gctgatacat cacgcttcct ccttacgcag gtagatttct ccgcctctgg cgcggaagtt
    16441 ctccgacata tccgccattc ccacttcaat ggtttgcgcg gcggcgtaat cacgtacttc
    16501 ctggctgatt ttcatcgagc agaatttcgg cccacacatg gagcaaaaat gggcgacttt
    16561 acctgactct tgcggcaggg tttcatcgtg ataagcgcgg gcggtaaacg ggtcgagggc
    16621 cagattaaac tggtcttccc agcgaaattc gaagcgggct ttcgacatgg cgttatcgcg
    16681 aatttgcgcg cctggatgcc ctttcgccag gtcagcggcg tggacggcaa tcttataggt
    16741 gataagcccc tgcttaacat cttctttatt aggcagaccc agatgctctt ttggcgttac
    16801 gtaacagagc atcgcgcagc caaaccagcc aatcatcgcc gcaccaatcc ccgacgtgaa
    16861 gtggtcatag cccggcgcaa tatcggtagt tagcggcccc agagtgtaaa acggcgcttc
    16921 gtggcagtgc tctaactcct cggtcatatt gcggcggatc atctgcatcg gcacgtggcc
    16981 tgggccttca atcatcacct gcacgtcata ttcccaggca attttggtca gttcgcccag
    17041 cgtatgcagc tcggcaaact gcgcttcatc gttggcgtcc tgaatagaac cggggcgcag
    17101 accgtcgccc agcgacagcg aaacgtcata agcggcacag atttcacaaa tttcgcggaa
    17161 gtgttgatag aggaaatttt cctgatgatg ggagaggcac catttcgcca taatcgaacc
    17221 gccgcgagag acgataccgg tcaggcgttt cgcggtcatc ggcacatagc gcagcagtac
    17281 gcccgcatgg atagtgaagt aatccacacc ttgctcggcc tgttccagca gcgtgtcgcg
    17341 gaacgcttcc caggtaagat cttcggcgat cccgttaacc ttctccagcg cctggtagat
    17401 cggcactgta ccgatcggca ccgggctgtt acgcaaaatc cactcgcggg tttcgtgaat
    17461 atagcgaccg gtggagagat ccatcaccgt atccgctccc cagcgcgtgg accataccag
    17521 cttttccact tcttcttcga tggaagaggt gaccgccgag ttgccgatat tggcgttaac
    17581 ttttaccagg aaattgcgac caataatcat cggctccgat tccggatgat taatgttggc
    17641 cgggataatc gcacgtccgg cagcaacttc atcacggaca aattccgcag tgatattttc
    17701 cggcagatgt gcgccaaagc tcattcccgg atgctggtgg cgtaaaacct cgctacggat
    17761 gcgctcgcgg cccatattct cgcggatggc gatgaattcc atttccggcg tgatgatgcc
    17821 ctggcgggcg tagtgcagtt gggtgacacg gcgtcctgtt ttggcgcgtt ttggcgttaa
    17881 taagccgcta aaacgtagct cgtcgagacc atcatctgcc agccgcgttc tggtgtaatc
    17941 ggaactgcgc acggtaagtt cttcggtatc gccgcgcgca tcgatccacg gctggcgtag
    18001 ttttgccagc ccttgctgca cgttaatggc gatctgcgga tcaccatacg ggccggaggt
    18061 gtcgtagacc ggaatcgctt cgttttcttc gtactgcggc tgttctttgc taccgccaat
    18121 tagcgtcggg ctaagctgga tctcacgcat cggcacgcgc acgccggggt gtgtgccagt
    18181 gatataaatg cgttttgagt tgggaaaggc ggtgccttcc agggtgtcga taaaatgttg
    18241 ggcccgggcg cgttgttcgc ggcgggtcag ttttgttgca gacatagctc attccaaaaa
    18301 gtcaaggacg tggcttgtca gacgacggat gaagcaagag acgatcgccg caaaggcgat
    18361 gcgatagcag attaactctt gttcccttcg caggtattag cctgatcagg ttccgcggat
    18421 cccgaataaa cggtctcagc cagttaaggc actccgacaa gaaattagcc cccacaaacg
    18481 gggcgttgaa tgtaaattac gcgttaacag cgcagaactc aagcaggatg tttgacgcgg
    18541 gcggcgtcaa gcaccagcag aatcagctta tcttccagca caaagcgcgc ttccagcgcc
    18601 tcgccgatgt cagataaaac ctgttgaaac tcaaggtaat tatcatgatc gatggcggtt
    18661 tccaggctgg aatcgtagta atccataatc tgttgggtat tggcttcgag ttgcggccaa
    18721 atcttcgcgg cgcgcgcgag ttgcccgttg ccttccagct tatgaagaat gcgctcataa
    18781 atactgaaat gtccggcaga caagtaatcg accaggctct gacaaaaatc atcaagggct
    18841 ttttcgttta gcctcatgta cgattctttg ccaggcttaa tgccaaccag attgtagtaa
    18901 gccacgagca gatgcttacg tacatgtagc cagcgatcaa ccagtttgtt acttcctctg
    18961 acgcgctccg tcaggttatc gagctggtta agcatgattg actccgcaag tttgtattca
    19021 aaaactgctc agtgagaaat gtaaaaacca tgttaaacat gccagtgatg caaaggtagt
    19081 gcaagagcta tggatcgtat aattgaaaaa ttagatcacg gctggtgggt cgtcagccat
    19141 gaacaaaaat tatggttgcc gaagggagaa ttgccatatg gcgaagcggc aaatttcgat
    19201 cttgtgggtc agcgcgcact acaaatcggc gaatggcagg gggaacctgt ttggttagta
    19261 caacagcagc ggcgtcacga tatggggtcg gtacgtcagg tcattgatct cgatgttggg
    19321 ctgtttcaac tggccggacg aggcgtacaa ctggcggagt tttaccgatc gcataaatac
    19381 tgtggttact gcgggcatga aatgtatccg agcaaaaccg aatgggcgat gctgtgcagc
    19441 cattgccgtg agcgttacta cccgcaaatc gccccctgca ttattgttgc catccgtcgc
    19501 gatgattcga tcctcctcgc ccagcatacc cgccatcgta acggtgtcca tacagtactt
    19561 gccggattcg tcgaagtggg cgaaaccctc gagcaggcag tcgcgcggga agtgatggaa
    19621 gagagcggaa ttaaagttaa aaacttgcgt tacgtgactt ctcagccgtg gccgtttcct
    19681 cagtctttaa tgaccgcgtt tatggcggaa tatgacagcg gcgacatcgt gatcgacccg
    19741 aaagaattgc tcgaggcgaa ctggtatcgc tatgacgatt tgccgttact cccgccgccc
    19801 ggcaccgtag cgcgccgtct gatagaagat actgtggcga tgtgtcgggc agagtatgag
    19861 tgatgataca ctgaccgcct gacgcactaa ggaacagcca aaatgaccga acttaaaaac
    19921 gatcgttatc tgcgggcgct gctgcgccag cccgttgatg tcactccagt atggatgatg
    19981 cgccaggcgg gtcgctatct accggaatat aaagccacgc gcgcccaggc gggcgatttt
    20041 atgtcgctgt gcaaaaacgc cgagctggcg tgcgaagtga ctttgcaacc gctgcgtcgc
    20101 tacccgctgg atgcggcgat cctcttttcc gatatcctca ccgtgccgga cgcgatgggg
    20161 ttagggctct attttgaagc cggagaaggt ccgcgtttta cctcgccagt cacctgcaaa
    20221 gccgacgtcg ataaactgcc aattccggac ccggaagatg agctgggtta cgtgatgaac
    20281 gcggtgcgta ccattcgtcg cgaactgaaa ggcgaagtgc cgctgattgg tttttccggc
    20341 agcccgtgga cgctggcgac ctacatggtg gaaggcggca gcagcaaagc gttcaccgtg
    20401 atcaaaaaaa tgatgtatgc cgatccgcag gcgctgcacg ctctactcga taaactggcg
    20461 aaaagcgtca ctttgtatct gaatgcgcag attaaagccg gtgctcaggc agtgatgatt
    20521 ttcgacacct ggggcggtgt gcttaccggg cgcgattatc aacagttctc gctctattac
    20581 atgcataaaa ttgttgatgg tttactgcgt gaaaacgacg gtcgccgcgt gccagtcacg
    20641 ctgtttacca aaggcggcgg acagtggctg gaagcgatgg cagaaaccgg ttgcgatgcg
    20701 ttgggcctcg actggacaac ggatatcgcc gatgcgcgcc gccgtgtggg caataaagtc
    20761 gcgttgcagg gtaatatgga tccgtcgatg ctgtacgcgc cgcctgcccg cattgaagaa
    20821 gaagtagcga ctatacttgc aggtttcggt cacggcgaag gtcatgtctt taaccttggt
    20881 cacggcattc atcaggatgt gccgccagaa catgctggcg tgttcgtgga ggcagtgcat
    20941 cgactgtctg aacaatatca ccgctaagga gtgattatgg atctcgcgtc attacgcgct
    21001 caacaaatcg aactggcttc ttctgtgatc cgcgaggatc gactcgataa agatccaccg
    21061 gatctgatcg ccggagtcga tgtcgggttt gagcagggcg gagaagtgac gcgagcggcg
    21121 atggtgctgc tgaaatatcc ctcgcttgag ctggtggagt ataaagttgc ccgcatcgcc
    21181 accaccatgc cttacattcc aggttttctt tccttccgcg aatatcctgc gctgctggca
    21241 gcgtgggaga tgctgtcgca aaagccggat ttagtgtttg tcgatggtca tgggatctcg
    21301 catcctcgcc gtcttggcgt cgctagccat tttggtttga tggtggatgt gccaacgatc
    21361 ggcgtggcga aaaaacggct ctgcggtaaa ttcgaaccgc tctccagcga accgggcgcg
    21421 ctggccccac tgatggataa aggcgagcag ctggcctggg tctggcgcag caaagcgcgc
    21481 tgtaacccgt tgtttatcgc taccggccat cgggtcagcg tggacagcgc gctggcgtgg
    21541 gtacaacgct gcatgaaagg ctatcgtctg ccggagccaa cgcgctgggc ggacgcggtg
    21601 gcctcggaac gtccggcgtt cgtgcgctat acagcaaatc agccctaatt caggtaaact
    21661 gcggataatt tccgtatttg agaactcaac atgttacaaa acccaattca tctgcgtctg
    21721 gagcgcctgg aaagctggca gcatgtcact ttcatggctt gcttatgcga acgcatgtac
    21781 cccaattacg ccatgttctg ccagcaaacc ggttttggtg atgggcaaat ttaccgtcgt
    21841 attctcgatc tcatctggga aacgctgacc gttaaagacg caaaagtaaa tttcgacagc
    21901 caactggaga aatttgaaga agcgattcct tcagccgacg atttcgatct gtacggcgtt
    21961 tatccggcaa tcgatgcctg cgtggcgtta agtgaactgg tccattcgcg tttgagtggt
    22021 gaaacgctcg aacacgcggt ggaagtgagt aagacctcca tcacgaccgt tgcgatgctg
    22081 gaaatgactc aggctggtcg cgaaatgagc gatgaagagc ttaaagaaaa cccagctgta
    22141 gagcaagaat gggacattca gtgggaaata ttccgacttt tagctgagtg cgaagaacgc
    22201 gatatcgagc tgataaaagg ccttagggca gacctgcgtg aggcgggtga gagcaatatt
    22261 ggtataattt ttcagcaata agaccagaaa acgtgattta acgcctgatt tgtcgtacct
    22321 ggagtcttcc ctttcgcccc ccgtctggtc tacatttggg gggcgaaaaa aagtggctat
    22381 cggtgcgtgt atgcaggaga gtgctattct ggcatttccg tcgcactcga tgcttagcaa
    22441 gcgataaaca cattgtaagg ataacttatg aacaagactc aactgattga tgtaattgca
    22501 gagaaagcag aactgtccaa aactcaggct aaagctgctc tggagtccac tctggctgca
    22561 attactgagt ctctgaaaga aggcgatgct gtacaactgg ttggtttcgg taccttcaaa
    22621 gtgaaccacc gcgctgagcg tactggccgc aacccgcaga ccggtaaaga aatcaaaatt
    22681 gccgcagcta acgtaccggc atttgtttct ggcaaggcac tgaaagacgc agttaagtaa
    22741 gattgcgtgg cagtgaacag ttttaacgaa ggggtggttt cacccctttt gtctttctgg
    22801 cgtcgatcat tgatgctggc tggcgctctg cttctcactg cctgtagtca taactcttca
    22861 cttcctccct ttaccgccag tggatttgct gaagaccagg gcgcggtacg catctggcga
    22921 aaagacagcg gcgataatgt gcatctgctt gccgtgttta gcccgtggcg cagtggcgat
    22981 accacgacgc gagagtatcg ctggcagggc gataacctca cgctcatcaa tatcaatgtt
    23041 tacagcaaac cgccggtgaa tattcgcgcg cgttttgacg atcgcggtga tctgagcttt
    23101 atgcaacgtg aatccgatgg ggaaaagcag cagctttcta acgaccaaat cgatttatac
    23161 cgttatcgtg ctgatcagat ccgccagatt agcgatgcct tacgtcaggg gagagtcgtg
    23221 ctgcgccagg ggcgctggca tgcgatggaa cagaccgtga ccacctgcga agggcaaacc
    23281 attaaacctg atttagattc gcaggcgata gcgcatatcg agcgccgcca gagccgctct
    23341 tctgttgatg tcagcgtggc atggctggaa gcgcccgaag gttcgcaatt actgttagtg
    23401 gcaaactctg atttctgtcg ctggcaaccc aacgagaaaa cgttctgatt taccagtggc
    23461 ccatacccat atgaccgcca ccaccgcagc cgccgtagcc catgcccatt ccggctccgc
    23521 gcggaatacc cgcttcagcc atcgcgatat ctcgtttcac ccgtaactca tctaacgact
    23581 gacgcaaatt ctccatctct ttggcgaccg cgttaatttt gctgctatcc ggtgggttcg
    23641 cggctaacag ggcattgtat tcataacgct tcgtcaccag ttgctgttgc agtgcgctgc
    23701 tttgagcgta aaagtcatta tggattttct gccacgctgt ctgttgttcg ctggtcaaag
    23761 gcgcggcatt ttgctgccac ataccgtgtc cgccgtgagc aaatgcagat gtcgatccca
    23821 tcgccattgc tgaaagcgcc atcattacca gggcaatttt cgtgttccgt ttcatggtta
    23881 atcctccagt ggttgtctta cttcgggtat tgcatcttcc gtgccaacga tgaaacgctg
    23941 atatgacggg taatctggca tgataaacga gtaaaaatga ctcgcctgct gcgggtagcg
    24001 agtcattttt actcattgaa acttaagcct ttgtgttaca gcgcagggta agcgctgata
    24061 aaagatggca tgatttctgc tgtcagaaag ggatgagcag gcaaagaaga agatgcgttt
    24121 tatgcaacgt tctaaagact ccttagctaa atggttaagc gcgatcctcc ccgtggtcat
    24181 tgttgggctg gtagggttgt ttgcggtgac ggtgattcgc gattatgggc gcgagactgc
    24241 cgccgccaga caaacgctgc tggaaaaagg cagtgtactt attcgcgctc ttgaatccgg
    24301 ctcgcgcgtc ggcatgggga tgcgcatgca tcatgcgcag cagcaggcct tactggaaga
    24361 aatggccggg cagcctggag tacgttggtt tgcggtcacg gatgaacaag gaacaatcgt
    24421 gatgcatagc aactccggca tggtgggaaa acagctttat tccccgcagg aaatgcagca
    24481 gttacatccg ggagatgaag aagcgtggcg gcggatcgat agcgcagacg gcgagcctgt
    24541 tctggaaatt tatcgccagt ttcaaccgat gtttgctgct ggaatgcacc ggatgcgcca
    24601 tatgcagcag tatgccgcga caccacaagc aattttcatc gctttcgacg ccagtaatat
    24661 tgtgagtgcc gaagatcgtg agcagagaaa caccctgatt atcctcttcg ccctggcgac
    24721 ggtcttgctg gcaagcgtat tgtcattctt ctggtatcgc cgctatttgc gctcgcgcca
    24781 gcttctacaa gatgaaatga agcgcaaaga gaagctggtg gcgctggggc atcttgcggc
    24841 aggcgttgcc cacgaaatcc gtaacccact ttcctcgatt aaaggactgg cgaaatactt
    24901 tgccgagcgc gcgcctgcag ggggagaagc gcatcaactg gcgcaggtga tggcgaaaga
    24961 ggccgaccgt ttaaaccgcg tggtaagcga gctgctggaa ctggttaagc caacgcatct
    25021 ggctttgcag gcggtggatc tcaacacgct gattaaccac tcattacagc tggtaagcca
    25081 ggatgcgaac agccgggaga tccagttacg ctttaccgcc aacgacacat taccggaaat
    25141 tcaggccgat ccggacaggc tgactcaggt cctgttgaat ctctatctca atgctattca
    25201 ggcgattggt cagcatggcg tgattagcgt gacggccagc gaaagcggcg cgggcgtgaa
    25261 aatcagcgtt accgacagcg gtaagggaat tgcggcagat cagcttgatg ccatcttcac
    25321 tccgtacttc accactaaag ccgaaggcac cggactgggg ctggcggtcg tgcataatat
    25381 tgttgaacaa cacggtggta caattcaggt cgcaagccag gagggaaaag gcgcaacgtt
    25441 caccctctgg cttccggtca ctattacgcg taagggccca caaggatgac gcacgataat
    25501 atcgatattc tggtggtgga tgatgacatt agccactgca ctattttgca ggctttactg
    25561 cgcggctggg gctataacgt cgcgctggcg aacagcgggc gacaggcgct ggagcaggtg
    25621 cgggaacagg tttttgatct tgtgctttgc gatgtgcgaa tggcggagat ggacggcatt
    25681 gccacgctaa aagagatcaa agcgttaaat ccggcgattc cggtgctgat tatgaccgca
    25741 tactccagcg tcgagacggc ggtagaagcg ttgaaaaccg gggcgctgga ttatctcatc
    25801 aagccgctgg atttcgataa cctgcaggcg acgctggaaa aggcgctcgc gcatacgcac
    25861 agtattgacg ctgaaacgcc tgcggtgtct gccagccagt tcggtatggt cggtaaaagc
    25921 ccggcgatgc aacacctgct cagtgaaatc gccctcgtcg cgccatcgga agccacggtg
    25981 ctgatccacg gcgattccgg caccggtaaa gagctggtcg ccagggcgat tcacgccagt
    26041 agcgcacgta gcgaaaaacc gctggtaacg ctcaactgtg cggcactcaa cgaatccttg
    26101 ctggaatctg aattgttcgg tcacgaaaaa ggggcgttca ctggagccga tagacgccgg
    26161 gaagggcgct ttgttgaggc ggacggcggc acgctgtttc tcgatgaaat tggcgatatc
    26221 tcgccgatga tgcaggtgcg tctgctgcgt gcgattcagg agcgcgaagt tcagcgtgtc
    26281 ggtagcaacc agactatctc ggttgatgtc cggttgattg cggcgaccca tcgcgatctt
    26341 gccgcagagg tgaatgccgg gcgttttcgc caggatctct actatcgcct gaatgtggtg
    26401 gcgattgaag taccatcgct gcggcaacgg cgggaagata tccctctgct ggctgtccat
    26461 tttcttcagc gctttgccga gcgtaatcgc aaggcggtaa aaggctttac gccacaggcg
    26521 atggatttgc tgatccacta cgactggccg ggaaatattc gtgaactgga aaacgcggtg
    26581 gaacgggcgg tggtgctgct gaccggggaa tatatttccg aacgcgagct gccgctggcg
    26641 attgccggta cgccgatccc actggggcaa agtcaggata ttcagccgct ggtggaagtc
    26701 gaaaaagaag tgattctggc ggcactggag aaaacgggcg gcaacaaaac cgaagccgcc
    26761 cgtcagttag ggatcacgcg caaaacgcta ttggcaaaac tgtcgcgtta gttctgctcg
    26821 cgttcgatag cgcgccagcc gatatctttc cggcagaagc agtcgtccca gtgaatatcg
    26881 gtcattaagg cataagcgcg tttctgcgct tctgccacgg tatgacccag cgcggtgacg
    26941 cacagtacgc gcccgccgct ggtcactacc tgctcgttat ccgccagttt cgtgcccgcg
    27001 tggaacactt tgccgcctgc cacttcttcc agcggcaggc cgtggatcac atcgccggtg
    27061 cggtaatcgc ccggatatcc acccgcagcc atcaccacgc cgagagaagc acgctcatcc
    27121 cactctgacg ttttctcgtc cagtttgcct tcacaggccg ccaggcagag ctcaaccaga
    27181 tcggacttca tgcgcagcat aatcggctgg gtttctggat cgccaaagcg gcagttaaat
    27241 tcgataacct tcggattgcc ctgtttgtcg atcatcaggc ccgcgtagag aaaaccggtg
    27301 taggtgttgc cttccgccgc catgcctttc acggttggcc agatgatacg ttccatggtg
    27361 cgctgatgaa cttcatcggt tactaccggc gcgggggagt aagcgcccat cccgcccgtg
    27421 tttggtccgg tatctttatc gcctacgcgt ttgtgatcct ggctggtggc catcggcagc
    27481 acatgctcgc cgtctaccat cacgataaag ctcgcttctt cgccgtcgag gaactcttcg
    27541 ataacgatgc gatgacccgc gtcgccaaaa gcgttgcccg ccagcatatc gtgaacagcc
    27601 gcttcggctt cttccagcgt catcgccacg ataacgcctt tcccggctgc cagaccgtcc
    27661 gctttaatga cgatcggcgc acctttctca cgcagatacg ccagcgcagg ttctacctcg
    27721 gtgaagttct ggtattccgc tgtagggatc ttatggcggg ccaggaaatc tttggtaaac
    27781 gctttcgaac cttccagttg cgcggcacct gcggttgggc cgaagatttt catcccggcg
    27841 gcgcggaagg tatcgaccac gcctttcacc agcggcgctt ccgggccgac gatggtcaga
    27901 tcaatctttt cgttttgtgc gaaatccaac agcgccggga tatcggtcac gccgatagca
    27961 acgttttgca gcgcgggttc cagcgcagtg cctgcattac ccggagcaac aaaaacagtt
    28021 tcaaccagcg gcgactgcgc cgctttccag gccagcgcgt gctcgcgccc gccgttacca
    28081 atcactaata ctttcatcta ttgctccatt aatggcggaa gtggcgcatg tcggtgaaga
    28141 gcatcgcaat accgtgctcg tcggcggcgg caatcacttc gtcatcacgg atagaaccgc
    28201 caggctggat tacgcaggtc acgcctgcag cggcggcggc atcaataccg tcgcggaacg
    28261 ggaagaacgc gtcagaagcc atcgaggaac ctttcacttc caagccttca tcggcagctt
    28321 taataccggc gattttcgcg gagtacacgc ggctcatctg gcccgcgcca atcccgatgg
    28381 tcatattgtt tttggcatag acgatagcgt tggatttcac gaacttcgcc actttccagc
    28441 agaacagcgc atcacgcagt tcctgttcgg tcggctgacg ttgggtgacg acgcgcagtt
    28501 cttccgcacc gaccatgccc aggtcacgat cctgaaccag cagaccgccg ttcacgcgtt
    28561 tgaaatcaag acccggaaca cgctcgcccc actgaccgca ggtcagaacg cgtacgttct
    28621 gcttggcggc ggtgattttc agggcttctt cgctggcgga aggcgcaata atcacttcga
    28681 caaactgacg agaaatgatg gcctgtgcgg tttccgcatc cagctcgcgg ttaaaggcaa
    28741 tgatgccgcc gaatgcggag gttgggtcgg ttttgtacgc gcgatcgtaa gcatcaagaa
    28801 tggaattgcc gatagccacg ccgcaagggt tggcgtgctt cacaatcaca catgccggct
    28861 cggcgaactc tttcacgcac tccagcgccg catcggtatc ggcgatgttg ttataagaga
    28921 gggctttacc ctgaacctgg gttgcggtag caacggaggc ttctttcaca ttctcttcta
    28981 tatagaaggc agcctgctgg tggctgttct cgccgtaacg catatcctgc ttcttaatga
    29041 agttcaggtt cagcgtgcgt gggaagcgac cggcggcttc tttgctttca ccgtggtaag
    29101 ccggaaccat gctgccgaag tagttggcaa tcatgctgtc gtaggcggca gtgtgttcga
    29161 aggctttgat ggcgaggtcg aaacgggttg caagcgtcag cgatccttcg ttgtcatcca
    29221 tctctttaat aatggcgtcg tagtcgctgc tcttcaccac gattgcgaca tctttatggt
    29281 tcttagcggc ggagcgcacc atggtcgggc cgccgatatc gatgttctca accgcatctt
    29341 ccagcgagca gccttcacga gcaacagtct gggcgaacgg atacaggtta acaaccacca
    29401 tatcgatagg ctggatctga tgttcttcca taatggcatc gtcctggccg cgacggccca
    29461 gaatgccacc atgtactttc ggatgcaggg tcttcacgcg tccatccatc atctccggga
    29521 aaccggtgta atcggaaact tcggttaccg gcagaccttt ttctgctaac agacgggcag
    29581 tgccccctgt agacagcagc tccacaccgc gtgcggaaag tgcctgggcg aattcgacga
    29641 taccggcttt gtcagaaaca ctgagcagag cgcggcggac tggacgacgt tgttgcatgg
    29701 taaatcccct ggatttgact attacagaga gcgttagctg aatttttcgc gaaaaactca
    29761 gctaacgccc ctaacggggc atccttattt ttcgcccgca ttgtaacgaa aacgtttgcg
    29821 caacgctcgc gaatttttct ctttcaatgg tgatcacaat tttgacagtg gttaccgtgg
    29881 gaaaaataca gaaattacat tgatgattgt ggataactct gtgcgtaaaa aggtataaag
    29941 cggggttttg ctggggaatg cagcagtcag tcatttttct gcaatttttc tattgcggcc
    30001 tgcggagaaa tccctataat gcgcctccat cgacacggcg gatgtgaatc acttcacaca
    30061 aacagccggt tcggttgaag agaaaaatcc tgaaattcag ggttgactct gaaagaggaa
    30121 agcgtaatat acgccacctc gcgacagtgc gctaaagcgc gtcgcaactg ctctttaaca
    30181 atttatca
//
LOCUS       DADMKF010000036        30002 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.037, whole genome shotgun sequence.
ACCESSION   DADMKF010000036 DADMKF010000000
VERSION     DADMKF010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30002
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.037"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            38..580
                     /locus_tag="JNC24_001482"
     CDS             38..580
                     /locus_tag="JNC24_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY9158259.1"
                     /translation="MKKSVLTAFITVVCATSSVMAADDNAITDGKVTFNGKVIAPACT
                     LVAATKDSVVTLPNVSATKLQTNGAVSGVKTDVPIALEGCDVTVTKNATFTFSGTADG
                     VQPTAFANQATTDAATNVALQMYLPDGSTSVTPGTETSNIQLADSAEQTVTFKVDYIA
                     TGKATSGNVNAVTNFHINYY"
     gene            663..1364
                     /locus_tag="JNC24_001483"
     CDS             663..1364
                     /locus_tag="JNC24_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HAY9158260.1"
                     /translation="MKTCITKGIVTVSLTAILLSCSSAWAAGKGGIGLAATRLVYSEG
                     EEQISLGVRNTSPDVPYLIQSWVMTPDNKKSADFIITPPLFVLNPANENLLRIMYIGA
                     PLAKDRETLFFTSVRAVPSTTKREEGNTLKIATQSVIKLFWRPKGLAYPLGEAPAKLR
                     CTSSADMVTVSNPTPYFITLTDLKIGGKVVKNQMISPFDKYQFSLPKGAQNSSVTYRT
                     INDYGAETPQLNCKS"
     gene            1389..3988
                     /locus_tag="JNC24_001484"
                     /pseudo
     CDS             1389..3988
                     /locus_tag="JNC24_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
     gene            3979..5049
                     /locus_tag="JNC24_001485"
     CDS             3979..5049
                     /locus_tag="JNC24_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY9158261.1"
                     /translation="MQIIFGEKCVLLLRLFFAAVLMLWCAQTAAYSGQCHTTQGNPYI
                     GVNFGVKTLEEEENTAGVVKDKFYQWNESNDYYVSCDCDKDNVRSGRWAFAADSPLVY
                     LGDNWYKINDYLAAKVLLQVKGSSPTAVPFENVGTGADTRWHICDPGGQRLGGQGASG
                     NSGSFSLKILQPFVGSVVIPPMALARLFECYNIPAGDSCTTTGTPVLVYYLSGTINSL
                     GSCSVNAGETIEVDLGDAFAANFRVVGHKPLGARTAELAIPVRCNTGNAGLVNVNLSL
                     TATTDPSYPQAIKTSRPGVGVVVTDSQNNIISPAGGTLPLSIPDDADSIARMNVYPVS
                     TTGVPPETGRFEATATVRINFD"
     gene            5061..5603
                     /locus_tag="JNC24_001486"
     CDS             5061..5603
                     /locus_tag="JNC24_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY9158262.1"
                     /translation="MKKETIFQCVILFFSILNIHVGMAGPEQVSMHIYGNVVDQGCDV
                     ATKSALQNIHIGDFNISDFQAANTVSTAADLNIDITGCAASITGADVLFSGEADTLTP
                     TLLKLTDTGGSGGMATGIAVQILDAQSQQEIPLNQVQPLTPLKAGDNTLKYQLRYKST
                     KAGATGGNATAVLYFDLVYQ"
     gene            5611..6126
                     /locus_tag="JNC24_001487"
     CDS             5611..6126
                     /locus_tag="JNC24_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HAY9158263.1"
                     /translation="MLKRIIWILFLLGLTGGCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFFHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            6092..6829
                     /locus_tag="JNC24_001488"
     CDS             6092..6829
                     /locus_tag="JNC24_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HAY9158264.1"
                     /translation="MPARHLYFIMTNTWNRLALLIFAVLSLLVAGELRAGVVVGGTRF
                     IFPADRESISILLTNTSQESWLINSKINRPTRWAGGEASTVPAPLLAAPPLILLKPGT
                     TGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRPEGLPG
                     DPLEAYQQLRWTRNSQGVQLTNPTPYYINLIQVSVNGKALSNAGVVPPKSQRQTSWCQ
                     AIAPCHVAWRAINDYGGLSAKKEQNLP"
     gene            6940..7950
                     /gene="pyrD"
                     /locus_tag="JNC24_001489"
     CDS             6940..7950
                     /gene="pyrD"
                     /locus_tag="JNC24_001489"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HAY9158265.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEEELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            8124..8666
                     /gene="zapC"
                     /locus_tag="JNC24_001490"
     CDS             8124..8666
                     /gene="zapC"
                     /locus_tag="JNC24_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HAY9158266.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKEQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(8663..9772)
                     /gene="ycbX"
                     /locus_tag="JNC24_001491"
     CDS             complement(8663..9772)
                     /gene="ycbX"
                     /locus_tag="JNC24_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HAY9158267.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDRWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVMPLKKSAMGDDGTILCCSCVPKTALKLAR"
     gene            10016..12124
                     /gene="rlmKL"
                     /locus_tag="JNC24_001492"
     CDS             10016..12124
                     /gene="rlmKL"
                     /locus_tag="JNC24_001492"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HAY9158268.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRTGWQPGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWVQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTGRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHMALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            12136..14043
                     /locus_tag="JNC24_001493"
     CDS             12136..14043
                     /locus_tag="JNC24_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAY9158269.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQEPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLLEKEEALRVEELQNAEFDRKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVDGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLDQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRALSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKTEEAAAAKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            14173..15426
                     /gene="pqiA"
                     /locus_tag="JNC24_001494"
     CDS             14173..15426
                     /gene="pqiA"
                     /locus_tag="JNC24_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HAY9158270.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSQQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            15431..17071
                     /gene="pqiB"
                     /locus_tag="JNC24_001495"
     CDS             15431..17071
                     /gene="pqiB"
                     /locus_tag="JNC24_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HAY9158271.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGLE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKVRLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            17068..17631
                     /gene="pqiC"
                     /locus_tag="JNC24_001496"
     CDS             17068..17631
                     /gene="pqiC"
                     /locus_tag="JNC24_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HAY9158272.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLASVWSQEAASIAQEIKRLP"
     gene            17887..18054
                     /gene="rmf"
                     /locus_tag="JNC24_001497"
     CDS             17887..18054
                     /gene="rmf"
                     /locus_tag="JNC24_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HAY9158273.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(18124..18642)
                     /gene="fabA"
                     /locus_tag="JNC24_001498"
     CDS             complement(18124..18642)
                     /gene="fabA"
                     /locus_tag="JNC24_001498"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HAY9158274.1"
                     /translation="MVDKRESYTKEDLLASGRGELFGAKGPQLPAPNMLMMDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDGRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(18711..20471)
                     /locus_tag="JNC24_001499"
     CDS             complement(18711..20471)
                     /locus_tag="JNC24_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lon protease family protein"
                     /protein_id="HAY9158275.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIANAARTLQSDAGQLVGGHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVTQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRAQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            20657..21109
                     /gene="matP"
                     /locus_tag="JNC24_001500"
     CDS             20657..21109
                     /gene="matP"
                     /locus_tag="JNC24_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HAY9158276.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(21185..22225)
                     /gene="ompA"
                     /locus_tag="JNC24_001501"
     CDS             complement(21185..22225)
                     /gene="ompA"
                     /locus_tag="JNC24_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HAY9158277.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     INNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGSVENGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKSNVYGKNHDTGVSPVFAGGVEYAITPEIATRL
                     EYQWTNNIGDAHTIGTRPDNGMLSLGVSYRFGQGEAAPVVAPAPAPAPEVQTKHFTLK
                     SDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLSER
                     RAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEIEV
                     KGIKDVVTQPQA"
     gene            complement(22580..23089)
                     /gene="sulA"
                     /locus_tag="JNC24_001502"
     CDS             complement(22580..23089)
                     /gene="sulA"
                     /locus_tag="JNC24_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor SulA"
                     /protein_id="HAY9158278.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLADDLTEEEHAELVDAANEGNAMGFIMRPVSASSHATRQLSG
                     LKIHSNLYH"
     gene            23308..23937
                     /gene="sxy"
                     /locus_tag="JNC24_001503"
     CDS             23308..23937
                     /gene="sxy"
                     /locus_tag="JNC24_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HAY9158279.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQN
                     SLVTEKILFMLEGAIIGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
     gene            complement(23900..26062)
                     /gene="yccS"
                     /locus_tag="JNC24_001504"
     CDS             complement(23900..26062)
                     /gene="yccS"
                     /locus_tag="JNC24_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HAY9158280.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGLLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQPYQHDPHFERAFTHIDAALERMRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPHNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWQFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNEALASLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQVPQETPVSA"
     gene            complement(26072..26518)
                     /locus_tag="JNC24_001505"
     CDS             complement(26072..26518)
                     /locus_tag="JNC24_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HAY9158281.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            26641..28695
                     /gene="helD"
                     /locus_tag="JNC24_001506"
     CDS             26641..28695
                     /gene="helD"
                     /locus_tag="JNC24_001506"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HAY9158282.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGERCDLDTTYRFNSRI
                     GKVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSDGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(28727..29185)
                     /gene="mgsA"
                     /locus_tag="JNC24_001507"
     CDS             complement(28727..29185)
                     /gene="mgsA"
                     /locus_tag="JNC24_001507"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HAY9158283.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(29281..29943)
                     /locus_tag="JNC24_001508"
     CDS             complement(29281..29943)
                     /locus_tag="JNC24_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HAY9158284.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
BASE COUNT         7731 a         7086 c         7880 g         7305 t
ORIGIN      
        1 gcattatcat atttacgaag agatcaggat gatgacgatg aaaaaaagtg tattgacggc
       61 gtttataact gtggtatgtg caacgtccag cgttatggca gctgatgata atgctatcac
      121 ggatggcaaa gtaacattta atggtaaagt tattgctcca gcttgtaccc tggtagctgc
      181 gacgaaagat tcagtagtca cattgccaaa tgtcagcgcc accaagttgc aaactaatgg
      241 agcagtctct ggcgttaaaa ctgatgtacc aattgcctta gaaggctgtg atgttactgt
      301 aactaaaaat gcgacgttta cctttagtgg tacggctgac ggcgtgcaac ctacagcatt
      361 tgccaaccag gctaccaccg atgcagcgac gaatgttgct ctacaaatgt atctgccaga
      421 tggttctaca tcggtaactc ccggtaccga aacgtcgaat attcagttag cggattctgc
      481 tgagcaaaca gtgaccttta aagttgatta tatcgctacg gggaaagcga cttctggtaa
      541 tgtgaatgcg gtaacaaatt tccatattaa ctattattaa tagaactcat taattgtttt
      601 attaattagt acccctctag tgttctggag gggatattca tattttttaa gagtgactat
      661 ttatgaaaac ttgcataaca aagggaattg tgaccgtaag tttaacggca atattactca
      721 gctgctcatc cgcatgggcg gcaggtaaag gcgggattgg acttgcagca acacgtcttg
      781 tttattcaga aggtgaagag caaatttcac tgggtgtacg taacaccagt ccggatgttc
      841 cttatcttat tcagtcatgg gtgatgaccc cagataataa aaaatcagca gattttatta
      901 ttacaccacc gctatttgtg ctgaatccgg ctaatgagaa tctgttacgc attatgtaca
      961 ttggtgcgcc gttggcgaaa gacagagaaa cccttttctt cactagcgta cgggcagtcc
     1021 cttcaacaac gaagcgggaa gagggaaata ccctgaagat tgccacacaa agcgtcatca
     1081 aacttttctg gcgaccaaaa ggtttagcgt atcccttagg cgaggctccg gcgaaactgc
     1141 gttgcacttc gtcagctgac atggttacgg tcagtaaccc aacaccttat ttcattaccc
     1201 tgacagacct gaaaataggg ggaaaggtag ttaaaaatca aatgatttcc ccctttgata
     1261 aataccaatt ctctctgcca aagggggccc aaaatagcag cgtaacgtat cgaaccatca
     1321 atgattacgg ggcggaaacg ccacaactca actgtaaatc gtaagccgtc ttcagttaag
     1381 agagcgagat gtatagaact catcgacaac acagcctgtt aagctctggt ggagtgccat
     1441 cgtttattgg tgggctggtg gtgtttgtgt cggcagcgtt caatgcacaa gctgaaacct
     1501 ggttcgatcc tgcttttttc aaagatgatc cctcaatggt ggccgatttg tctcgtttcg
     1561 aaaaaggaca aaaaataacg ccaggggttt atcgtgtcga tattgttctg aatcagacaa
     1621 ttgtagatac gcgcaacgtc aattttgttg agataacgcc agagaagggg attgccgcct
     1681 gtttgacgac tgaaagcctg gatgcaatgg gtgttaatac tgatgcgttt ccggctttta
     1741 aacaactgga caaacaagcg tgtgtgccat tggcggagat tattccggat gccagcgtaa
     1801 cgtttaatgt gaataaactc cgtctggaaa tttcagtacc gcaaatcgcc atcaaaagta
     1861 atgcccgtgg ttatgtcccc cctgaacgtt gggatgaagg gatcaacgcg ctattactgg
     1921 gatattcgtt tagcggggct aacagtattc atagcagcgc agacagtgat tctggcgaca
     1981 gctattttct gaatttaaac agtggcgtta atttaggccc atggagattg cgcaacaatt
     2041 caacatggag tcgcagtagt ggccaaaccg cagaatggaa gaatctcagc agctatttgc
     2101 agcgggcggt tattccacta aaaggcgaac tgaccgtagg tgatgattat actgcaggcg
     2161 attttttcga tagtgtcagc tttcgtggtg tgcagctggc gtcagatgac aacatgctgc
     2221 cagacagcct gaaagggttt gcgcctgtgg tgcgtggtat cgccaaaagc aatgcccaga
     2281 taacgattaa gcaaaatggt tacaccattt accaaactta tgtatcgcct ggtgcttttg
     2341 aaattagtga tctctattcc acgtcgtcga gcggtgattt gttagttgaa atcaaagaag
     2401 cggacggcag cgtcaatagc tacagcgtac cgttttccag cgtgccatta ctccagcgtc
     2461 aggggcgaat caaatacgcg gtgacactgg cgaaatacag aaccaatagt aatgaacagc
     2521 aggagagcaa atttgcctag gccacgttgc agtggggcgg accgtgggga acgacatggt
     2581 atggtggtgg acaatatgct gaatattacc gtgccgccat gtttggtctg ggatttaacc
     2641 ttggcgattt cggagcaatt tcgttcgatg cgacccaggc gaagagtacg ctggcagacc
     2701 aaagcgaaca taaaggtcag tcatatcgtt ttctgtatgc caaaacgctc aaccaattgg
     2761 gcactaattt tcaattgatg ggctatcgct attcgacgtc gggtttctac accctttccg
     2821 acaccatgta taaacatatg gatggctacg aatttaatga cggtgatgat gaagatacgc
     2881 cgatgtggtc gcgttattac aatttgtttt acaccaaacg tggcaaactg caggtcaata
     2941 tctcccagca attaggcgag tacggttcgt tttatttaag tggtagccag caaacttact
     3001 ggcataccga tcaacaggat cggctattac agtttggcta caacacgcaa attaaagatc
     3061 tctcgctggg gggttcctgg aactacagta agtcccgtgg tcaacctgat gctgatcagg
     3121 tgtttgcact aaatttttcc ctgccgctca atctgttgct ccccagaagt aatgatagct
     3181 ataccaggaa aaaaattacg cctggatgac ctctaacacc agcatcgata acgaagggca
     3241 cattacacaa aacctgggtt taacggagac actactcgat gacggtaacc tgagctacag
     3301 cgtgcaacag ggatataaca gcgaggggaa aacggctaat ggtagcgcca gcatggacta
     3361 caaaggggcg tttgcagatg cccgagtggg ctacaactac agcgataacg gcagtcaaca
     3421 acaactgaac tacgctcttt caggcagttt agttgcccat tcacagggca ttaccctggg
     3481 gcaatcgctg ggggaaacta acgtgctgat tgcagcacca ggcgcagaga atactcgtgt
     3541 ggcgaacagc accgggctga aaactgactg gcgcggatat accgttgtcc cctatgccac
     3601 ttcttatcgg gaaaatcgaa tcgcacttga tgcggcatcg ttaaaacgta acgtggatct
     3661 tgaaaatgca gtagtcaacg tggttcccac caaaggggcg ttggttctgg cggagttcaa
     3721 tgcccatgcg ggagcaaggg tattaatgaa aacatcaaag cagggtatac cgctgcgttt
     3781 tggcgcgata gcgacgctgg acggcgtaca gactaatagc ggcattattg atgatgatgg
     3841 ctcgctctat atgtccggtt tgcccgctca gggagcgata actgtacgct ggggcgaagc
     3901 tcccgatcaa atttgtcata tcagttacca gcttaccgaa caacaaatta actctgcgat
     3961 tacgcgaatg gatgccatat gcagataatc tttggagaaa aatgcgtgtt attactacga
     4021 ctattttttg ccgccgtctt aatgctatgg tgcgctcaaa ctgctgctta tagcgggcag
     4081 tgtcatacca ctcaggggaa tccgtatatt ggcgtcaatt ttggcgttaa aaccctggag
     4141 gaagaagaaa atacggctgg agtagttaaa gacaaatttt atcagtggaa cgaatcgaat
     4201 gattattatg tttcctgtga ttgcgataaa gacaatgtca gaagtggccg atgggcattc
     4261 gccgcggatt caccgttagt ctatttaggc gacaactggt acaaaattaa tgactatctt
     4321 gccgccaaag ttttattgca ggttaaaggc agttctccta ctgcggttcc tttcgaaaac
     4381 gtgggcacag gggcagatac acgatggcat atttgcgatc ccggcggtca acgtttaggt
     4441 ggccaggggg ctagcggtaa tagcggtagc ttttccctga aaatattgca gccgttcgtt
     4501 ggttcggtcg tcattcctcc tatggcgctg gcgcgattat ttgaatgcta caacataccc
     4561 gcaggtgatt cctgcacgac tacaggtaca ccggttttag tgtattacct gtctggtacg
     4621 atcaattcac ttggctcatg ttccgtcaat gccggagaaa caatcgaggt cgatctgggc
     4681 gacgcatttg cggctaactt tcgtgttgta gggcataagc ctcttggggc cagaacggca
     4741 gaacttgcaa ttccagtcag gtgtaacacg ggaaacgcgg ggttagttaa cgtcaacctg
     4801 agtctgacgg caaccacaga ccccagctat ccccaggcga ttaagacgtc acgtcctggc
     4861 gtgggcgtgg tggtgaccga tagccagaac aacattattt cccctgctgg tggaacatta
     4921 ccgctctcta ttcctgatga tgcagacagt atcgcgcgaa tgaatgtcta tccagtcagc
     4981 acgacaggtg taccaccaga aaccgggcga tttgaagcca cggcaacggt gagaataaat
     5041 tttgattaac gcgtgaacgt atgaagaaag aaacgatatt tcagtgcgtt atcttgttct
     5101 ttagcattct taacatccat gtcgggatgg ctgggcctga acaagttagt atgcatattt
     5161 atgggaatgt ggtcgatcag ggctgtgatg tcgccaccaa aagtgcatta caaaatattc
     5221 atattggtga ttttaatatc agtgattttc aggccgcgaa taccgtaagc actgctgctg
     5281 atttgaatat cgatatcacc ggttgtgccg ctagtattac tggcgcggac gtccttttta
     5341 gcggcgaggc tgacaccctt acgccgacac tgctcaaact aactgacaca ggcggaagcg
     5401 gtggtatggc aacggggatt gccgtgcaaa ttcttgatgc gcaaagtcag caagaaatcc
     5461 cgctcaatca ggtccagcct cttacgccct taaaagccgg ggataacaca ctcaaatatc
     5521 aacttcgtta taagtccaca aaggcgggag caacgggcgg taatgcgacg gcggttctct
     5581 attttgatct ggtttaccag tgaaggagtg atgttgaaac ggataatctg gattctgttc
     5641 ttactgggat taacgggggg ctgtgagcta tttgcccatg atggcacggt caacattagc
     5701 ggatcgtttc gccgtaatac atgcgtgctg gcacaggata gcaagcaaat caatgtgcag
     5761 ttaggcgatg tctcgctgac acgttttttt catgggaatt atggcccgga gaaatctttc
     5821 atcattaatt tgcaggattg cggaacagat gtcagcacgg ttgacgtcac cttttcagga
     5881 acccccgacg gagtgcagag cgaaatgctg tccattgaaa gtggtacgga tgcagccagc
     5941 gggctggcaa ttgcgattct ggatgatgcg aagatattga ttccgcttaa ccaggcgagt
     6001 aaagactaca gcctacatag cggtaaggtg ccattaactt tttatgcgca attgcgacct
     6061 gtcaatagcg atgtgcagtc gggtaaagtg aatgccagcg cgacatttgt acttcattat
     6121 gactaatacg tggaacagat tggcgctctt gattttcgcc gttttatcgc tgctggtggc
     6181 gggtgaattg cgggcaggag tggtggtcgg cggaacgcga tttatctttc cggcagacag
     6241 agaatcgata tctattttac tgactaatac cagtcaggaa tcctggctta ttaacagtaa
     6301 aatcaaccgc ccaacgcgtt gggccggggg ggaagcgtcg acagtgccag caccattact
     6361 ggccgctccg ccacttattc tcctgaagcc cggtactaca ggcacgttgc gcttgctgag
     6421 aacggaaagc gacatcttgc ctgtggatcg cgaaacgcta tttgagttaa gcattgccag
     6481 cgtgccatcc ggcaaagttg aaaatcagag cgtaaaagtg gcgatgcgct cggtatttaa
     6541 actgttctgg cgacccgaag gtttgccggg cgacccgctg gaagcttatc aacaattacg
     6601 ctggacacgg aattcgcagg gtgtacaact cactaaccca acgccttact acattaacct
     6661 gattcaggtg agcgtaaatg gtaaagcgtt aagtaatgcg ggagttgtgc cgcctaaaag
     6721 ccagcgtcag acaagctggt gtcaggcaat cgcaccgtgt catgtcgcct ggcgggcgat
     6781 aaatgattac ggcgggttga gtgcaaagaa ggagcaaaat ctgccctgaa acaggttcgg
     6841 aaaacgtttg cgtttttttt gccgcaggtc aattcccttt tggtccgaac tcgcacataa
     6901 tacgcccccg gtttgcacac cgggaatcca ggagagttca tgtactaccc cttcgttcgt
     6961 aaagcccttt tccagctcga tccagagcgc gctcatgagt ttacttttca gcaattacgc
     7021 cgtattacag gaacgccgtt tgaagcactg gtgcggcaga aagtgcctgc gaaacctgtt
     7081 aactgcatgg gcctgacgtt taaaaatccg cttggtctgg cagccggtct tgataaagac
     7141 ggggagtgca ttgacgcgtt aggcgcgatg ggatttggat cgatcgagat cggtaccgtc
     7201 acgccacgtc cacagccagg taatgacaag ccgcgtctct ttcgtctggt agatgccgaa
     7261 ggtttgatca accgtatggg ctttaataat cttggcgttg ataacctcgt agagaacgta
     7321 aaaaaggccc attatgacgg cgtcctgggt attaacatcg gcaaaaataa agatacgcca
     7381 gtggagcagg gcaaagatga ctatctgatt tgtatggaaa aaatctatgc ctatgcggga
     7441 tatatcgcca tcaatatttc atcgccgaat accccaggat tacgcacgct gcaatatggt
     7501 gaagcgctgg atgatctctt aaccgcgatt aaaaataagc aaaatgattt gcaagcgatg
     7561 caccataaat atgtgccgat cgcagtgaag atcgcgccgg atctttctga agaagaattg
     7621 atccaggttg ccgatagttt agttcgccat aatattgatg gcgttattgc aaccaatacc
     7681 acactcgatc gttctcttgt tcagggaatg aaaaattgcg atcaaaccgg tggcttaagt
     7741 ggtcgtccgc ttcagttaaa aagcaccgaa attattcgcc gcttgtcact ggaattaaac
     7801 ggtcgcttac cgatcatcgg tgttggcggc atcgactcgg ttatcgctgc gcgtgaaaag
     7861 attgctgcgg gtgcctcact ggtgcaaatt tattctggtt ttatttttaa aggtccgccg
     7921 ctgattaaag aaatcgttac ccatatctaa ttatttcttc gaattcctct ttataagata
     7981 atcagggctt tattttcagc cctggttgtt ttatattcat cgctgttgct tatttagaca
     8041 ttttgtactt ttattgttga ggttattaag cgaagcgaca atggattgtg ttgctgcggt
     8101 tttatagggt aggggagagg cagatgcgaa ttaaaccaga cgataactgg cgttggtatt
     8161 acgatgaaga gcacgatcga atgatgctcg atttagccaa tggtatgcta tttcgctcac
     8221 gttttgcgcg caagatgttg actccagatg ccttttctcc cgcaggcttt tgcgttgacg
     8281 acgccgcgct ctatttctct tttgaagaaa agtgccgcga ttttaattta tctaaagaac
     8341 aaaaagccga actggtgctg aatgcactgg tagcaattcg ctatctcaaa ccgcaaatgc
     8401 cgaaaagctg gcattttgtt tcccatggtg aaatgtgggt gcccatgccg ggcgatgcgg
     8461 cctgcgtttg gttgagcgat actcacgagc aggtcaattt gctggtcgtt gaatctggcg
     8521 aaaatgccgc actatgcctg ctggcacaac cctgcgttgt tattgcgggt cgcgcgatgc
     8581 agttgggcga cgccattaaa atcatgaacg acaggctgaa accgcaggtt aatgttgaca
     8641 gcttcagcct cgaacaggca gtctaacgcg ccaacttaag tgcagtcttc ggtacacagc
     8701 tacagcaaag aatggtgcca tcatcgccca ttgctgattt tttcagcggc atgacttcgc
     8761 cttctaaaag ctgaacacgg caacttccac aaatgcccgc gcggcaagaa taagggatac
     8821 gaattccctg attttctaat tgctccagca acacctgttg gttatttcca cgaaatgcct
     8881 gtccctgcca gtcaatatct acatttgcgt ccggttgttg cgtgatgttg gcggtatcat
     8941 cagcggcagc tgcgccgtaa atttttgccg gagccgttgc cagaatttcc acctcatcgc
     9001 caacgcgaat cacgccgcta ttacgggcaa ttaaattctg accaaaatcg acatcgccgt
     9061 tatcctgggc agtgcggaaa gattgcaatg tttttaatgg ttcgcctgcc ggatgttttt
     9121 gccctttttc tgggctgacg gtggtgaaaa tacagcggct acaaggttta accacatcaa
     9181 acaccacatc accaatgcga atcactttcc agcgatcttc ttcccaggct gacgcgccgg
     9241 aaaccaccag attggggcgg aattgctcca tttttacact ggccggacaa cgttgttgga
     9301 gatcacgtaa cgaggcttcg ttagcaagaa ggtaaggata gccatcagca aatgacagag
     9361 gtacagtgtt gtggcgtttc acgcgccggg tcatttgtgg ccccacccag cgtaattgca
     9421 cttcgcggga gaaaaatcca cttagccatt tgttgatcgc gtctggcgca attcgcgcgg
     9481 taaaatgtgt gccccaaact tcggttggtg cgtcttgtgt ggcgaaatca gcaaaacgaa
     9541 catatgcgct actgccatct ggtgcggtga gatgcaagcc atcatgcaca ggtgaaggag
     9601 taaaccgtac catctgggga aactggcgag cggtaataaa cgtaccgtca ggttccgtga
     9661 tcataaagat gcgatcgaag gccagaccac tgacatcagc cagagcatgt gtaagaccaa
     9721 tgccgcgcat cgatttaaca ggatgaataa aaagccgggt taatgtcgcc acggtgcggt
     9781 cctcaaatga aaataagccc tcaactttat gacatgacgc gcttattagc tataatgcgc
     9841 aacaattttc ttagcgcctg tcccattagg ctattttatt tgtcagtttg gccctgggtt
     9901 gtgctcgaaa tcctcacgta cttgtgtacg ctccggtttc tccgcgctgt ccatgtccag
     9961 actgactgca acaattacgc ctactgcggt aggctcttag agtaaaagtg acgatatgaa
    10021 ttctctgttt gccagtacgg cccgtgggct ggaagagctg ttaaaaactg aactggaaaa
    10081 cctgggggcc gttgaatgcc aggtggttca gggtggggtc catttcaagg gcgacacacg
    10141 gcttgtttac cagagcctga tgtggagccg cctggcctcg cgtattatgt tgccgctggg
    10201 cgagtgtaag gtttacagcg atttagacct ctatctcggt gttcaggcga tcaactggac
    10261 agagatgttt aatcctggcg cgaccttcgc tgtccacttc agtggtttga atgacaccat
    10321 ccgcaacagt cagtacggcg cgatgaaagt gaaagacgcg atcgtcgatg ctttcacgcg
    10381 gaaaaatctg ccgcgtccaa atgttgatcg cgatgcgccg gatatccgcg ttaacgtctg
    10441 gctgcataaa gaaaccgcca gtatcgctct tgatctgagt ggtgatggtt tacatctgcg
    10501 tggctatcgc gatcgtgctg gtattgcgcc gatcaaagaa accctggcag ccgctattgt
    10561 gatgcgaacc ggctggcagc caggaacacc gctgctcgat ccgatgtgtg gttccggtac
    10621 gttgctgatt gaagcggcga tgctagcgac cgatcgcgca ccaggcttgc accgtgggcg
    10681 ttggggcttt agcggctggg tgcagcatga tgaagctatc tggcaggaag tgaaagcgga
    10741 agcgcaaact cgtgcccgta aaggcctggc tgagtatagc tctcattttt acggttcgga
    10801 cagcgacgca cgggtgattc aacgtgcgcg cactaacgcc cgtcttgcgg ggattggtga
    10861 actgatcacc tttgaggtga aagatgtcgc gcaactgacc aatccgctgc cgaaagggcc
    10921 gtacggcaca gtgttgagca acccgccata cggggaacgt ctggacagcg aaccggcgct
    10981 gattgcgctg catagcctgc tgggtcggat catgaaaaac cagtttggtg gctggaatct
    11041 ctctttgttt agtgcctcgc cggatctgct aagctgcttg cagctgcgtg cagacaaaca
    11101 gtacaaggcg aaaaacggcc cgctggactg cgtacagaaa aattaccacg ttgccgaaag
    11161 cactccagac agcaaaccgg cgatggtagc ggaagactac gccaaccgcc tgcgtaagaa
    11221 cctcaaaaaa ttcgagaagt gggctcgcca ggaaggaatt gaatgttacc gcctgtatga
    11281 cgccgatcta ccggaatata acgttgccgt tgaccgttat gccgactggg tggtggtgca
    11341 ggagtatgcg ccgccaaaaa ctattgatgc tcacaaagcg cgtcagcgtc tgttcgatat
    11401 tatcgctgca accatttcgg tactggggat tgcgccaaac aaactggtgc tgaaaacccg
    11461 tgaacgccag aagggcaaaa accagtacca gaaactgggc gagaagggcg agtttcttga
    11521 agttaccgaa tataacgctc acttgtgggt gaacctgacg gattatctcg atactggtct
    11581 gttcctcgat caccgtatcg cccgccgtat gctcggtcag atgagcaaag gcaaagattt
    11641 cctcaacctg ttctcttata ccggtagcgc caccgtgcat gcgggattag gcggcgcgcg
    11701 cagcaccacc accgtggata tgtcgcgtac ttatctggag tgggcagaac gcaacctgcg
    11761 tctgaatggc ctgaccgggc gtgcgcatcg cctgattcag gccgattgcc tggcgtggct
    11821 gcgtgaggca aatgaacagt ttgatctgat ctttatcgat ccgccaacct tctctaactc
    11881 aaaacgaatg gaagatgcgt ttgatgttca gcgcgatcat atggcgctga tgaaagattt
    11941 gaaacgtctg ctgcgtgcag gtgggacgat catgttctcg aacaacaaac gtggcttccg
    12001 tatggatctc gacggcctgg caaaactggg actgaaagca caagaaatta cgcaaaaaac
    12061 gctctcccag gatttcgccc gtaaccgcca gatccacaac tgctggctga ttaccgcagc
    12121 ctgaaaggaa tagtaatgtc attaatcagt atgcatggcg catggctgtc gttcagcgac
    12181 gcgccgcttc tcgataacgc agaactgcat atcgaagata acgaacgtgt ttgtctggtg
    12241 ggtcgcaacg gcgcgggcaa atcgacgtta atgaaaatcc tcaaccgtga acaagggctg
    12301 gatgacggtc gcattattta cgagcaagat ttgattgtag ctcgtctgca acaggagccg
    12361 ccgcgtaacg ttgagggtag cgtttatgat ttcgttgccg aaggcattga agaacaagcg
    12421 gaatatctga aacgctatca cgatatttcg cgcctggtga tgaacgaccc gagcgagaaa
    12481 aatctcaacg aactggcgaa ggttcaggaa cagttggatc accacaacct gtggcagctg
    12541 gaaaaccgca tcaacgaagt gctggcgcaa ctggggttag atcctaatgt tgcgctgtcg
    12601 tcgctttccg gcggctggtt gcgtaaagcg gcattaggac gcgcgctggt gagtaatccg
    12661 cgcgtgctgt tgcttgatga accgacaaac cacctggata ttgaaaccat cgactggctg
    12721 gaagggttcc ttaaaacctt caatgggacg attattttca tctcccacga ccgttcgttt
    12781 atccgcaata tggcgacgcg cattgttgat ctcgatcgcg gcaagctggt gacctatcca
    12841 gggaattacg accagtacct gcttgaaaaa gaagaagccc tgcgcgtgga agaactacaa
    12901 aatgccgagt tcgatcgcaa actggcgcag gaagaggtgt ggatccgcca ggggatcaaa
    12961 gcacgccgta cccgtaatga aggccgcgta cgcgccctga aagcaatgcg tcgcgagcgt
    13021 ggtgaacgtc gcgaagtgat gggtaccgca aagatgcagg tggaagaggc cagccgctcc
    13081 ggtaagatcg ttttcgaaat ggaagacgtt tgctaccagg ttgacggtaa gcaactggtg
    13141 aaagattttt cggcccaggt tctacgtggc gacaaaattg ccctgattgg tccgaatggg
    13201 tgcggcaaaa ccacgctgct gaaactgatg ctcgatcagc ttcaagcgga cagcgggcgt
    13261 attcacgttg gcaccaaact ggaagtggct tatttcgatc agcaccgcgc ggaactggat
    13321 cccgataaaa cggtgatgga taaccttgcc gaaggtaagc aagaggtgat ggttaacggc
    13381 aagccacgcc acgtattggg ctatttgcag gactttctgt tccatccgaa acgggcgatg
    13441 acgccggtac gtgcgctttc tggcggtgag cggaaccgct tgctgctggc gcgtttgttc
    13501 ctcaaaccaa gcaacttatt gattcttgac gaaccgacca acgatcttga tgtcgaaacg
    13561 ctggaactgc tggaagaact gatcgacagc tatcagggca cggtattgct ggttagccac
    13621 gatcgtcagt ttgtcgataa caccgttaca gaatgttgga tcttcgaagg cggcggtaaa
    13681 attggtcgtt atgtcggcgg ttatcatgat gcccgtggtc agcaagagca gtatgtggcg
    13741 ctcaaacagc ctgcggtgaa aaaaaccgaa gaagccgccg cggcaaaagc agaaactgta
    13801 aaacgcagca gtagcaaact aagctataaa ttgcagcgcg aactggagca gctaccgcaa
    13861 ttgctcgaag atctggaggc gaagctggaa gccctacaga cgcaggtggc ggatgcttcc
    13921 ttcttcagtc agccacatga gcagacacaa aaagtgctcg ccgatatggc tgctgcagag
    13981 caggagctgg agcaagcctt tgaacgctgg gagtatcttg aagcgttaaa aaatggtggc
    14041 tgatcgcaaa atagtcgatt taaacagaga cagctgacaa cggtaaaatt gcctgatgaa
    14101 ctgggtttat caggcatgaa aaccaacact ttgttagcca tctgtaaaat agcgcatcat
    14161 taaggagtac caatgtgcga acatcatcat gccgcgaagc acatcctgtg ctcgcagtgt
    14221 gacatgctgg tggcgttacc gcgccttgag catggtcaga aagcggcatg tccccggtgt
    14281 ggcacaacgt taaccgtggc gtgggatgcc cctcggcagc gtccgaccgc ctatgcgttg
    14341 gctgcactgt tcatgctgtt gctgtccaac ttgtttcctt ttgtgaatat gaacgttgca
    14401 ggagttacca gtgaaattac attactggaa attcccggcg tgcttttttc tgaggactac
    14461 gccagcctcg gcaccttttt cctgttgttt gtgcaactgg ttcccgcgtt ttgtctgata
    14521 accattctgt tactggtgaa tcgcgcggaa ttaccggtcc gtttaaaaga gcaactggca
    14581 cgggtgcttt ttcaactcaa aacctgggga atggcggaga ttttcctcgc tggtgtgctg
    14641 gtcagtttcg ttaaactgat ggcttacggc agcattgggg taggcagcag ctttctcccc
    14701 tggtgtttat tttgtgtcct gcaactgcgc gcttttcagt gcgttgatcg tcgctggtta
    14761 tgggacgaca tcgccccgat gccagaactg cgccagccgc taaaaccagg cgtcacgggg
    14821 atacgtcagg ggctgcgttc gtgctcctgt tgtacggcaa tccttcctgc tgatgaaccc
    14881 gtgtgcccgc gttgtagtac caaagggtac gttcggcgta gaaacagcca gcagtggaca
    14941 ctcgcgctgc ttgtaacgtc catcatgctg tatcttccgg ctaatatttt gcccatcatg
    15001 gtgacggatt tattaggctc gaagatgccg tcgacgattc tcgctggggt cattctgtta
    15061 tggagcgaag gctcttatcc cgtcgctgcg gtgatctttc tggccagtat tatggtgcca
    15121 acgttaaaga tgatcgccat cgcgtggctg tgttgggatg ccaaagggca tggcaagcgc
    15181 gacagtgaaa gaatgcattt gatttatgaa gttgttgagt ttgtaggccg ctggtcgatg
    15241 attgacgttt tcgttatcgc ggtgctctcg gcgctggtgc gtatgggagg tttaatgagt
    15301 atttatccgg caatgggtgc attaatgttt gctttagtcg tcataatgac aatgttttct
    15361 gctatgacgt ttgacccgcg tttgtcgtgg gatcgtcaac ctgaatcaga gcatgaggag
    15421 tcctgacagt atggaatcta ataatgggga agccaaaatc cagaaagtga agaactggtc
    15481 tcccgtgtgg atatttccta tcgtcacggc gctcattggg gcctgggttc ttttttatca
    15541 ttacagccat cagggactgg aagtgaccct gatcaccgcg aatgcggaag gaattgaagg
    15601 tggcaaaacc accattaaaa gccgtagcgt tgacgtcggc gtggttgaaa gcgccacact
    15661 ggctgatgat ttgacgcacg ttgaaatcaa agtgcggctg aattctggta tggaaaaatt
    15721 gctgcataaa gacaccgtct tttgggtggt gaaaccgcag attggtcgcg aagggattag
    15781 cggcctggga acgctgctgt ctggagttta tatcgaactg cagccaggcg cgaaaggcag
    15841 caaaatggat aaatacgatt tgctggactc gccaccgttg gccccgcctg atgcgaaagg
    15901 tatccgtgtg attctcgata gcaaaaaagc cgggcagctc tcgccaggag atccggtgct
    15961 gttccgtggc tatcgggtag gttcggttga aaccagcacc ttcgatacgc aaaaacgcaa
    16021 tatcagttat caactgttca tcaatgcacc ttatgaccga ctggtgacca gcaatgttcg
    16081 cttctggaaa gatagtggca ttgcggttga tctgacgtca gcggggatgc gtgtggagat
    16141 gggctcattg acaacgctgc ttagtggcgg tgttagcttt gatgtgccgg aaggtctgga
    16201 tttagggcag ccagtggcac cgaaaacagc tttcgttttg tatgatgatc agaagagcat
    16261 tcaggattcg ttgtacaccg atcacattga ttatctgatg ttctttaaag attcggtacg
    16321 cggtctgcaa ccgggagctc cggtagaatt ccggggtatt cgcctgggta ccgtaagcaa
    16381 agtgccattc tttgcgccga atatgcgtca gacatttaac gatgattacc gtattccggt
    16441 actgattcgt atcgagccag agcggctgaa aatgcagctt ggcgaaaatg cggatgttgt
    16501 tgagcacctt ggcgaattgt tgaaacgtgg tttacgcgga tcgctgaaaa ccggaaacct
    16561 ggtcactggt gcactgtatg ttgatcttga tttctatcca aatacgcctg caataaccgg
    16621 tattcgtgaa tttaatggtt atcagattat cccgactgtt agcggcggcc tggcgcaaat
    16681 ccagcaacga ctgatggaag cgttggataa gatcaacaaa ctgccattga atccgatgat
    16741 tgaacaggca accagtacgc tttctgaaag tcagcgcaca atgaaaaacc tgcaaacgac
    16801 gctggatagc atgaacaaga tcctcgccag ccagtcgatg cagcagttgc cgacggatat
    16861 gcagtcaacg ttgcgtgaat tgaatcgcag catgcagggc ttccagcctg gctccgcagc
    16921 ctacaacaag atggtggcgg atatgcagcg ccttgatcag gtgttgcgag aactgcaacc
    16981 ggtgctgaaa acgctcaatg agaagagtaa cgcgctggta tttgaagcga aggacaaaaa
    17041 agatccagag ccgaagaggg cgaaacaatg aaaaagtggc tagtgacgat tgcagcactg
    17101 tggctggccg gatgcagctc cggcgaaatt aataaaaact attaccagtt acctgtggtg
    17161 cagagcggta cacaaagtac cgccagccag ggcaatcgtc tgttatgggt agagcaggtc
    17221 actgttcctg actatctggc ggggaatggt gtggtttatc aaaccagtga tgtgaagtat
    17281 gtgattgcca acaacaactt gtgggccagc ccgttggatc aacaattgcg caacaccctg
    17341 gttgccaacc tgagtacgca actgcccggc tgggtggttg cctcccagcc tctgggaagc
    17401 gcccaggaca cgctcaatgt taccgtaacg gagtttaacg gtcgctatga tggcaaggtc
    17461 attgtcagtg gtgagtggct gttgaaccac cagggacaac tgatcaaacg tccgttccgt
    17521 ctggaaggag tgcaaactca ggatggttac gatgagatgg ttaaagtact ggccagtgtc
    17581 tggagtcagg aagccgcttc tattgcgcaa gagataaagc gtctacctta attataaaga
    17641 tttgtaaata taaccgtctc cggtatgttg cctgaggcgg tttttttgtt tctaacgtgc
    17701 ggaaaaagtt gttcctcttt acattttttg tacaaccgac atacccgtgt agctcacaaa
    17761 tatgacagtg gcgtgaattt tgcgcattga cggcagttat gattcgcggt attgcttaac
    17821 tgtgattgca catttagtaa tcactgtttt cttttccacc agaaaccagt atgagggaaa
    17881 cgaggcatga agagacaaaa acgagatcgc ctggaacggg cacatcaacg tggttatcag
    17941 gccggcatcg ccggacgctc aaaagaaatg tgtccctatc agacgctgaa tcaaaggtca
    18001 caatggctgg gaggctggcg agaagccatg gcggacaggg tagtaatggc ctgattctgt
    18061 ctctttaaaa agaaacctcc gcattgcgga ggtttcgcct tttgatactc tgtctgatta
    18121 taatcagaag gcagaagtat cctggaacag accgactttc aggtcgctgg cggtatagat
    18181 caaacgacca tcaaccagca cttctccatc cgccaggccc ataatcagac gacggttaac
    18241 aatgcgttta aagtgaatac ggtaggtcac ttttttcgct gtcggcagta cctgaccagt
    18301 gaatttcact tcgccaacgc ccagtgcgcg gcctttacct tcgccgccca gccagccgag
    18361 gtagaaccct accagctgcc acattgcgtc caggcccagg catcccggca taaccggatc
    18421 gccaataaag tggcatccga agaaccacag atccggattg atatccagtt ctgcttcaac
    18481 ataccctttg tcgaagttac cacccgtttc ggtcattttg accacacggt ccatcatcag
    18541 catattcggt gctggcaatt gcgggccttt agcgccaaac agttcaccgc gaccagaggc
    18601 aagaaggtct tcttttgtat aggattcgcg tttatctacc atgttctctg taagccttat
    18661 tttattgaag cacgcaggat agctaacacg tgtacgctga acaagtccga tcagttcgga
    18721 ataaaccagt tcagccaacg taatggccat ggaaaacggt gacgtccttc ctgttgcgat
    18781 gcttgcgcga tacgttcctg gatggtttgc atcagcgtcg tttggccttc gccatcccac
    18841 accagattta ataataacgg cagtgcgtca gtcacatcgt ctactgccca gatggtgaat
    18901 ttgccttctt ctaccgcttt cactagttca ctgtgaagac ttaaatggcg aacgttagct
    18961 gtggggataa tgacaccttg tttcccggtt aactcacgtt gctggcaaat agcaaagaag
    19021 ccttcgattt tctcatttaa accaccgacc ggctgggcgc gaccgaactg atcgactgaa
    19081 cctgtgatag cgatactctg attcaccggc acatcggcga gggcgcttat cagggcgcag
    19141 agttcagcca tcgaggcact atctccatca acttcactgt atgactgctc aaatgtcagc
    19201 gatgctgaga aggggatctg ttgctcaagc tgtagttccg acatcaggaa cgcttgcatg
    19261 atcatcatcc ctttcgcatg gatattgccg ccaagctccg ctttgcgttc gatgtcggtg
    19321 aattcaccat cgccaatatg cacaacgcag ctaatgcgag aaggttcgcc aaaagcgcgt
    19381 ggatgacccg gaaattcaat gaccgaaagg gcgttaattt gcccgatgcg ttcgccttcg
    19441 gtttcaatca ggatttgctc ctgaaggatc tcatcctgca tacgttcagc gaggaaacct
    19501 tcgcgccatt cacgctgctg cagcattaag tttagctgct cgccggagaa ggtgtcgcca
    19561 tcacacaggg aggcgacctc tttacactgg cggaggatcc actgcgggct aagcggaagt
    19621 gtttcttgtt caccggtgta gcgtgctgct tcgcggataa gtaccggcca ggcatccgct
    19681 cccggtgcag gtaagtgatt atgtctggcg gtaaatgtca cccagcgaca ccactgggtt
    19741 actgactccg catcgacaat ctgcagagta tcttcaaatt cgctataaat agcctgctct
    19801 gaaagctctg gctccatctc ctggaaatca gccaatgatt cgcgttcgcc taccagaatg
    19861 actttcagct tcaatggcat cgaaggcaca gagacgggga gagggcgcga ctcatcaaac
    19921 gcaacccagt caaaacgctc gcggttaacg atatttttca gccgcatcca cagcagaggt
    19981 tgcgccagca gtgtacgcaa agagatgatg agaataccgc catttgcctg atgcaccaga
    20041 ccaggctgca gggtaatgtc gccattaaac tggcgcaggc agccaaagag ttgctccgct
    20101 tctacccagt cggcagcgac aacttgcgtt aaagtcgcaa aattatcatc tgcactcact
    20161 gcgtgacgta agcggatgga gtggccggaa acctcatagt gaccgcccac cagttggcct
    20221 gcatcgcttt gtagcgtacg cgcggcattg gcaataagat tcagatactc agactcttcc
    20281 ggggccttcg ccagcataaa agaggaggat gctcgcgtat gcagcaactg ctccagcgca
    20341 aattgcagcc gcggttgagt atcactgaat aaaggatcgt tttcgtcaat caaatgtggc
    20401 tgagcaaata tttcctgata gctatcggta tcaggaacca ggtcacgcca tgcaagtttc
    20461 gtaatggtca aagttgatgt tttttagtct gttgtcaaag ccgcgattat accgtaaccg
    20521 gcactacagc acacgtagaa agcaccgaca atactccagg catgggcgtt aaagctcaca
    20581 ggatggagat tcttttcttc actggcctaa aaagctgata ttctgtaaag agttacacgg
    20641 taacattgag atcgctatga aatatcaaca acttgaaaat cttgaaagcg gttggaaatg
    20701 gaagtatctg gtgaagaagc atcgcgaagg ggagttaatc acccgttaca tagaagccag
    20761 tgccgcccag gaagccgtcg atgtgttgct ctcgctggaa aatgaacccg tgctggtaaa
    20821 tggctggatt gacaagcata tgaatccgga actggtcaat cgcatgaagc aaaccattcg
    20881 ggcaagacga aagcgccatt ttaatgcgga acaccagcat acgcgcaaaa aatcgatcga
    20941 tctggaattt attgtctggc aacgtctggc tggtcttgcg cagcgtcgcg gtaaaacgct
    21001 ttctgaaacg attgttcagc tgattgaaga tgcggaaaac aaagagaaat acgcgaataa
    21061 aatgtcttct ctgaagcagg atctgcaggc attgctgggt aaggaataac tgacgaaagt
    21121 cagttcaatt tactaaaggc aaaaaaaacc ccgcagcagc ggggtttttc taccagacga
    21181 gaacttaagc ctgcggctga gttacaacgt ctttgatacc tttaacttcg atctctacgc
    21241 gacgatccgg agccaggcag tcgatcagtg cagcacgctg tttcacgttg tcacaggtgt
    21301 tgccagtaac cgggttggat tcgcccatac cacgtgcgga gatcttgtct gccgggatac
    21361 ctttggagat caggtaatca acaacagact gagcacggcg ctcggacaga ccctggttgt
    21421 aagcgtcaga accgatgcgg tcggtgtaac ccagaacaac tacggaaccg tctttcggat
    21481 ccaggttgct cagctggctg tacagctgat ccagagcagc ctgaccttcc ggtttcaggg
    21541 ttgctttgtt gaagttgaac agaacgtcag acttcagagt gaagtgcttg gtctgtactt
    21601 ccggtgccgg agctggagcc ggagcaacta ctggagctgc ttcgccctga ccgaaacggt
    21661 aggaaacccc caggctcagc atgccgttgt ccggacgagt gccgatggtg tgtgcgtcac
    21721 cgatgttgtt ggtccactgg tattccagac gggtagcgat ttcaggagtg atcgcgtact
    21781 caacaccgcc agcgaagacc ggagaaacgc cggtgtcgtg gtttttacca taaacgttgg
    21841 atttagtgtc tgcacgccat accataccac ccagacgagt gtagatgtcc aggtcgtcag
    21901 tgattgggta acccagttta gcggtcagtt gaacgccctg agctttgtat gcaccgtttt
    21961 caacgctgcc tttgtacggc atacgaccta accagtcgta acccatttca aagccaacat
    22021 acgggttaac ctggtaacca ccaaaagcac cagcgcccag ttggttttca tgggtcgggc
    22081 cattgttgtt gatgaaacca gtgtcatggt actgggacca gcccagttta gcaccagtgt
    22141 accaggtgtt atctttcgga gcggcctgcg ctacggtagc gaaaccagcc agtgccactg
    22201 caatcgcgat agctgtcttt ttcatttttt gcgcctcgtt atcatccaaa atacgccatg
    22261 aatatctcca acgagataac acggttaaat ccttcaccgg gggatctgct caattaactc
    22321 taccgatatc ttcggcttat gccgagcacc cctggcgatg taaagtctac aacgtagttg
    22381 aaaacttaca agtgtgaact ccgtcaggca tatgaaaaaa aagtcttgta taaggtatgt
    22441 ttaatctttt ttgtcagcga caatttacag aagaaaatcg cggaaaccgc ttcagacaag
    22501 cctccgcaag gaaaattagt cacgactgaa agcattggct gggcgacaaa aaaagttcca
    22561 ggattaatcc taaatttact taatgataca aattagagtg aatttttagc ccggaaagtt
    22621 gtctcgtggc gtgagaggat gcgcttaccg gacgcataat aaaccccata gcgttacctt
    22681 catttgccgc atcaacaagt tcagcatgct cttcttcagt caaatcatct gccaaccaac
    22741 cgatcaccac actgtaattg cccgtgcgta aagcgcgaac cattgactcc acagtgtggc
    22801 aaggggagag ctggctaatc tgcattactt tcgttaaggg tagcccagat gcctgaaccc
    22861 attcccgact cagtttttgt tgcggtgtta accagagttg ccagcgcgat tgctgaccga
    22921 gttgctgtaa caatggcaac agtagaagtt gcgtcatcat gggctgatct tcgcgataga
    22981 caacttcact gataagcccg gctgtagtgt tttccgtaga gacacgcgca attttacttg
    23041 ctgcggatga gaacgacgaa gaacgatgtg catagcctga agtgtacata atcaatccag
    23101 cccctatgag ttactgtatg gatgtacagt acatccagtg acaacaaaga tcaaccctat
    23161 tttcggaaag agcctcgcaa attttgtcgt tggtgacggg aaaacataaa ttaatcttgc
    23221 cccttaagaa taagttgcct attttcgtag ttaacggatc cgttaatgtg aatcattctt
    23281 ttatgttatg attttaaaag gaattttatg aaaagcctct cctataagcg gatctataaa
    23341 tcacaagaat acctggcaac gttgggcaca attgaatacc gatcattgtt tggcagttac
    23401 agcctgaccg ttgacgacac ggtgtttgcg atggtttctg atggtgagtt gtatcttcgg
    23461 gcttgtgagc aaagtgcaca gtactgtgta aaacatccgc ctgtctggct gacatataaa
    23521 aagtgtggcc gatccgttac cctcaattac tatcgggttg atgaaagtct atggcgaaat
    23581 caactgaagc tggtgcgtct gtcgaaatat tctctcgatg cagcgctgaa agagaaaagc
    23641 acgcgcaata cccgggaaag actgaaagat ttgcccaata tgtcttttca tctggaagcg
    23701 attctcgggg aggtggggat taaggatgta cgggcgttac gtatacttgg ggcaaaaatg
    23761 tgttggttgc gactgcggca gcaaaacagt ctggtgacag aaaagattct gtttatgctt
    23821 gaaggtgcca ttatcggcat tcatgaagct gcgctcccgg tggcacgccg ccaggagctt
    23881 gcagaatggg ctgactctct tacgccgaaa caggagtttc ctgcggaact tgagtaatct
    23941 ggcgttgcag acgaccaatc tcaggcagta atgcaatcaa taatccaact tgttgtacga
    24001 ccagaggttc tttgctgtct gcccgtggtt caagttgctg catccgctgt ttcaggctag
    24061 ctaatgcctc attgacgcgt tcttcatcag caggttgatg atgtaacgcg tcatcaacat
    24121 agcaaactgc gtcatcaaga aacgccagaa tttcaggatt agttaactgc tcccggtgag
    24181 caccgagggc tgagatatag ctggtaaacg tatggttaag gcacagcaac cgaaacgcgg
    24241 cttcgcgaat ttgcggggta acgttcggct cgctggacat atttgatacc accgacgcca
    24301 gctcagcatc acggttgtgt gcatcgcggc gggcaatacg atacgccaga cggttatcac
    24361 gcccctgatg gtattgctcc agtatggcat cgagataccg acagttggcc tctgtggcgc
    24421 gttcgagcat gcgcggcaga ttgcgaaact gccagtcagg ccagatgtag ctcactgccg
    24481 cccacgcaat ggcacaacca atcagcgtat cgattacgcg aggtaacgct acttcaaaac
    24541 cttcacccag taagttaaaa cacagtagca ccaaaagtgt gatgaacatc gttgcatgag
    24601 cgtattgcac gttacggaag gcaaaaaaga gcacgccggt aataaccagc agcaccagct
    24661 gcccttccag tgatggcaca aaccacagca caggaatgcc aatggcgata cctaccagcg
    24721 taccaataat ccttaacttc aggcggtggc gcgtggcgtt atagtttggc tggcagacaa
    24781 acaaacttgt cagcaagatc caatacccgt gatgcattcc ggttatctga atgatggcgt
    24841 agccgaagca caataccagc gacattctta ccgcatgacg gaagagggcg gattccggcg
    24901 tgaagtgacg gctaagacgc agccagatat cactcaaccc gtgcgggctg tcatcagcga
    24961 gctcattttc gtcattatta tggggtagtg cctgggcctg ttctgattca attgttgcca
    25021 gttgggcatc aatggcgcgt aaattgttca gcaaaaatcc cagtgttttg agtaaatcgg
    25081 cgggtgcgcc gttatcgcgc atccgctcca gcgcagcatc aatatgcgta aaagcgcgct
    25141 caaaatgcgg atcatgttga taaggctgac gcaacaaaat acagcgtgac agttgctggc
    25201 acgcctggcc ctgcatcgac atcagccgct gaaaacggaa cagcacgtcg ctgtggcgaa
    25261 aatgttcacg caatgtttga tactgaatat gagaagagct ggcacgctcg tgaatatcct
    25321 gtgcgacaaa gtaataatgc agcgtgcgac gcgttccccg ttgaccacga tcgccacgta
    25381 agcgggtcag cagcgagagt ttcgtctgat tcaatgtcgc catcagcaga ccgttggcga
    25441 gagccaaatc gtacagcggt gcctggcttt catcttcaat atcaggatca aacatgcgcg
    25501 acttgagctc aagataacgc gccagttgtt catagcaacg cgccaggttg tcctgcagcg
    25561 ggcggaccgg gaacagcaga tgaccaataa gtgtcaggac gttgtaccag acggcaccgg
    25621 ccagcagata catcggctgc tgataccagt gctcatacag tgatgttccc aacatagtgt
    25681 aaatggcgat cagcaatgca ccgaaggcaa ttgttgcata gcgttgaccc agaccgccga
    25741 gcaaaatgaa gccgctggta gagagcgtta agccaatcgc aaatagccag ggccagggaa
    25801 acagcaattc tactgaggcc gaggcgataa aaaagcagaa cagcgtaatg atgaggttac
    25861 gcaaacgtcc cgccagtcgg tcatcgagat cggtcagcgc cgctgccacc atccccagcg
    25921 ttagcggaat cgtcagtttt acatcaccca gccaccacgg aaacgctgtg gttccacaaa
    25981 gcgcaataaa aatacgcgcg taatacagcc aggcgctgtt ccaggtatag cgtttgagca
    26041 aaggacttag cataaaggcc atattagtgg attattcaaa acgacgacgt gcattggctt
    26101 cacgcgcagc ttgcgctgtt tctaccgata ccacgcgacg accaaccggc catagtgcaa
    26161 tagcggcaat tttaaagttc gcaatgccga caggaatgcc aatgattgaa atacattgtg
    26221 cgatgcccgt tgcaatgtgc atcaggcata accaccagcc aaagaaaatc agccagaaaa
    26281 tattcaatac cgtaccgcca gtattcagca gcacattttt gccagccggg ttcagttcat
    26341 cgacatgaat agcttcattg ccataaggca ccagagatag tttagtgatc tcccagcagg
    26401 atcgtgtcag cggtaaggta aaaatcagca caatactgac cagagtcgcc aacagccagc
    26461 ccagagtggt ggcaaatccg ccaagcacaa agttcagaat gttcaaaacg gtacgcataa
    26521 aacctcgctt tactgtggtt ttcagtaatg ggcggcaatt gtaacgtttt tttgggctgg
    26581 agcacgtttt ctctgacggt tacactgata agaaataatt tcgtgtggat ctacagagtc
    26641 atggaactga aagcgacaac gcttggaaaa cgtctggcac agcaccctta cgatcgggcg
    26701 gtgatcctca atgccgggat taaagtctcc ggcgatcgcc acgaatacct tattcctttc
    26761 aatcaattac tggcgattca ctgtaagcgc ggtctggtat ggggcgagct ggaatttgta
    26821 ctgccggacg aaaaagtggt gcgtctgcac ggcaccgaat ggggcgagac gcagcgtttt
    26881 taccatcatc ttgatgctca ctggcggcgg tggagtggcg agatgagcga aattgcgtct
    26941 ggtgttttac gccagcaact ggatttgatt gccacgcgca ctggagaaaa taaatggctg
    27001 acgcgtgagc aaacctctgg cgttcagcaa caaatccgcc aggctttgtc ggcgttgccg
    27061 ttgccggtta accgactgga agaattcgat aactgccgtg aggcgtggcg taaatgtcag
    27121 gcctggttga aagatattga aagcgctcgg ttgcagcata accaggcgta taccgaagcc
    27181 atgcttaccg agtatgcgga ttttttccgc caggtcgagt cttcaccgct gaatccggcg
    27241 caggcccggg cagtcgttaa tggcgagcat tctctgttag tgctggcagg tgcaggaagc
    27301 ggaaaaacgt cggtgctggt ggcccgtgca ggctggttgc tggcgcgtgg tgaagcgtcc
    27361 cctgagcaaa ttttattgct ggcgtttggt cgcaaagccg ctgaagagat ggacgagcgg
    27421 attcgcgaac ggctacatac cgaagacatt accgcacgca cgtttcatgc gctggcgctg
    27481 catattattc agcagggcag caaaaaagtt ccgatagtca gcaaactgga aaatgatacc
    27541 gctgcccgtc atgaactctt tattgctgag tggcgcaagc aatgcagcga aaagaaagcg
    27601 caggcgaagg gctggcggca atggctgacg gaagaaatgc agtggtcagt gccagaaggt
    27661 aacttctggg atgatgaaaa attacagcgt cgccttgcct ctcgcctcga tcgctgggta
    27721 agtctgatgc ggatgcacgg tggtgcacag gcagaaatga ttgccagtgc acccgaagag
    27781 attcgcgatc tgttcagtaa acgtatcaag ttgatggccc cgttattaaa agcctggaaa
    27841 ggtgcgctga aggcagaaaa cgctgtcgat ttttcgggcc ttattcatca ggcgattgtg
    27901 attctggaga aaggtcgctt tatcagcccg tggaagcata ttctggttga tgaatttcag
    27961 gatatctcgc cgcagcgggc agcgttgtta gcggcattac gcaagcaaaa cagtcagacg
    28021 acgttgttcg ctgttggtga tgactggcag gcgatttacc gattcagcgg tgcgcaaatg
    28081 tcgctcacca ccgctttcca tgaaaacttt ggtgaaggcg aacgttgtga tttagacacg
    28141 acttaccgtt ttaacagtcg tatcggtaag gtggcaaacc ggtttattca gcagaaccca
    28201 ggccagctga aaaagccgct aaacagctta accaatggag acaaaaaagc cgtcacgtta
    28261 ttggatgaga gtcaacttga cgctttgctg gataagctct ctggttatgc caaaccggaa
    28321 gagcgcattc tgatcctggc gcgttaccat cacatgaggc ctgccagcct ggaaaaagcg
    28381 gcaacacgct ggccgaagtt gcaaatcgac tttatgacca ttcatgccag caaagggcaa
    28441 caggcggatt acgtcatcat cgttggcttg caggagggaa gtgatggttt tccggctgcg
    28501 gcgcgggagt cgattatgga agaggcgcta ctgccaccgg ttgaggattt cccggacgct
    28561 gaagaacggc ggttaatgta cgtggcgctg acccgggcac gccatcgggt atgggcactg
    28621 tttaacaaag agaatccctc tccctttgtg gaaatactga aaaatctgga tgtgccggtg
    28681 gcgagaaaac cgtaagaaac aggtggcgtt tgccacctgt gcaatattac ttcagacggt
    28741 ccgcgagata acgctgataa tcggggatta gaatatcgac cgcgtcgttg aaatgcggcg
    28801 actggattat gaagtctgcc gttgccacgt tggtggcgac cggaatgttc cataccgtcg
    28861 ccagacgcag caaggctttc acgtcaggat cgtgcggcac ggcatttagt ggatcccaga
    28921 agaaaatcaa tacatcaatt ttcccttctg agatcaatgc gccaacctgc tggtcacccc
    28981 ccattgggcc actcaacatc gcgttgacgt tcatgccggt cgcgcgggaa attaagttac
    29041 cggtagtgcc tgttgcatac agtacgtgtt gttccagtaa cggttgatgc cgttccaccc
    29101 agctcatcag catttgtttg cagtgatcgt gtgccaccag cgcaatatgt ttccgcgcag
    29161 gtaaagtgcg agtcgtcagt tccataatgt acatccgtag ttaactttcc tacagattac
    29221 tgtaagcact tatcgctgca agataaagac cgaaaaagcc tgcgcacagg cacaaaaatc
    29281 tcaggaagat ggttgttttt ccgcccactg caggaaagta tttcgcgttt gtgggtcagc
    29341 cagtttaaac caatacttca gccgttgttc tgtgagcacc tgagactgcg gtggaatagc
    29401 atccagctcg gaaacacccg aaagcagcgt actgtcatct gccatcatcg tggcaaattg
    29461 cggtagtgaa gcgcgtttcg cggatttgtt atagcgttca acctcgacct cataatcaat
    29521 cgtttttgcc gttgaggtaa tggcgagaat atccagcttt accggaatcg gcgtcgcatc
    29581 gccatccaac aattcaaggc gcggcgcggc atcaaaatgg ttagcttccc gctcattctc
    29641 caggcgaggc aggcgaaaat tgacctggtt gatgagctgg gtattaaaac tgaccaccag
    29701 cggtggggag atatacagcc gttcttcact attggaaaga tgaatcgtct tctcaacgcg
    29761 aaacactaac tgatgcggtc cattatccag ttcaatgctg tccgcaccgc gcagcagaga
    29821 gctggaaacc ttcttgccgt cgagtaccag aagatcgaca tcggttgaaa gccgcagcgt
    29881 ggtggcaaaa acggataccg gcaaacataa tgcaattaag gtggtcacga tgccggtttt
    29941 cataggtagc tcctgtcaaa agaccgcccg aataataatg tgtcaaaatt tttctttaat
    30001 ca
//
LOCUS       DADMKF010000037        29548 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.038, whole genome shotgun sequence.
ACCESSION   DADMKF010000037 DADMKF010000000
VERSION     DADMKF010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29548
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.038"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            <1..689
                     /gene="dgt"
                     /locus_tag="JNC24_001509"
     CDS             <1..689
                     /gene="dgt"
                     /locus_tag="JNC24_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dNTP triphosphohydrolase"
                     /protein_id="HAY9158285.1"
                     /translation="EDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRS
                     NSLSRSTEDQFFMYLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLL
                     KLYKNVAVKHVFSHPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRF
                     PIESRLFHKLSTRHRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWD
                     EYRRLMAVEQ"
     gene            819..2243
                     /gene="degP"
                     /locus_tag="JNC24_001510"
     CDS             819..2243
                     /gene="degP"
                     /locus_tag="JNC24_001510"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HAY9158286.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKNLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            2398..3555
                     /locus_tag="JNC24_001511"
     CDS             2398..3555
                     /locus_tag="JNC24_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family transcriptional regulator"
                     /protein_id="HAY9158287.1"
                     /translation="MAGWHLDTKMAQDIVARTMRIIDTNINVMDARGRIIGSGDRERI
                     GELHEGALLVLSQGRVVDIDDAVARHLHGVRQGINLPLRLEGEIVGVIGLTGEPENLR
                     KYGELVCMTAEMMLEQSRLMHLLAQDSRLREELVMNLIQAEENTPALTEWAQRLGIDL
                     NQPRVVAIVEVDSGQLGVDSAMAELQQLQNALTTPERNNLVAIVSLTEMVVLKPALNS
                     FGRWDAEDHRKRVEQLITRMKEYGQLRFRVSLGNYFTGPGSIARSYRTAKTTMVVGKQ
                     RMPESRCYFYQDLMLPVLLDSLRGDWQANELARPLARLKAMDNNGLLRRTLAAWFRHN
                     VQPLATSKALFIHRNTLEYRLNRISELTGLDLGNFDDRLLLYVALQLDEER"
     gene            complement(3644..4030)
                     /locus_tag="JNC24_001512"
     CDS             complement(3644..4030)
                     /locus_tag="JNC24_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HAY9158288.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(4192..5004)
                     /gene="yaeI"
                     /locus_tag="JNC24_001513"
     CDS             complement(4192..5004)
                     /gene="yaeI"
                     /locus_tag="JNC24_001513"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase YaeI"
                     /protein_id="HAY9158289.1"
                     /translation="MISRRRLLQATAATIATSSGFGYMHYWELGWFELIRHRLAFFKD
                     NAAPFKILFLADLHYSRFVPLSLISDAIALGIEQKPDLILLGGDYVLFDMPLNFSAFS
                     DVLSPLAECAPTFACFGNHDRPVGTEKNHLIGETLKSVGITVLFNQATVIATPNRQFE
                     LVGTGDLWAGQCKPPPASEANLPRLMLAHNPDSKEVMRDEPWDLMLCGHTHGGQLRVP
                     LVGEPFAPVEDKRYVAGLNAFGERHIYTTRGVGSLYGLRLNCRPEVTMLELV"
     gene            complement(5059..5883)
                     /gene="dapD"
                     /locus_tag="JNC24_001514"
     CDS             complement(5059..5883)
                     /gene="dapD"
                     /locus_tag="JNC24_001514"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HAY9158290.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(5914..8586)
                     /gene="glnD"
                     /locus_tag="JNC24_001515"
     CDS             complement(5914..8586)
                     /gene="glnD"
                     /locus_tag="JNC24_001515"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HAY9158291.1"
                     /translation="MNTLPEQYANTALPTLPGQPQNPCVWPRDELTVGGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPIDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPEARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLHYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(8648..9442)
                     /gene="map"
                     /locus_tag="JNC24_001516"
     CDS             complement(8648..9442)
                     /gene="map"
                     /locus_tag="JNC24_001516"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HAY9158292.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFIEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            9810..10535
                     /gene="rpsB"
                     /locus_tag="JNC24_001517"
     CDS             9810..10535
                     /gene="rpsB"
                     /locus_tag="JNC24_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HAY9158293.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIASRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFEKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            10793..11644
                     /gene="tsf"
                     /locus_tag="JNC24_001518"
     CDS             10793..11644
                     /gene="tsf"
                     /locus_tag="JNC24_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HAY9158294.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            11791..12516
                     /gene="pyrH"
                     /locus_tag="JNC24_001519"
     CDS             11791..12516
                     /gene="pyrH"
                     /locus_tag="JNC24_001519"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HAY9158295.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            12808..13365
                     /gene="frr"
                     /locus_tag="JNC24_001520"
     CDS             12808..13365
                     /gene="frr"
                     /locus_tag="JNC24_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HAY9158296.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            13457..14653
                     /gene="ispC"
                     /locus_tag="JNC24_001521"
     CDS             13457..14653
                     /gene="ispC"
                     /locus_tag="JNC24_001521"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HAY9158297.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEDVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYDRYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANAREVARKE
                     VMRLAS"
     gene            14842..15600
                     /gene="ispU"
                     /locus_tag="JNC24_001522"
                     /gene_synonym="uppS"
     CDS             14842..15600
                     /gene="ispU"
                     /locus_tag="JNC24_001522"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HAY9158298.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGRIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            15613..16470
                     /gene="cdsA"
                     /locus_tag="JNC24_001523"
     CDS             15613..16470
                     /gene="cdsA"
                     /locus_tag="JNC24_001523"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HAY9158299.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            16482..17834
                     /gene="rseP"
                     /locus_tag="JNC24_001524"
     CDS             16482..17834
                     /gene="rseP"
                     /locus_tag="JNC24_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HAY9158300.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVMALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            17864..20296
                     /gene="bamA"
                     /locus_tag="JNC24_001525"
     CDS             17864..20296
                     /gene="bamA"
                     /locus_tag="JNC24_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HAY9158301.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            20418..20903
                     /gene="skp"
                     /locus_tag="JNC24_001526"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             20418..20903
                     /gene="skp"
                     /locus_tag="JNC24_001526"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HAY9158302.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            20907..21932
                     /gene="lpxD"
                     /locus_tag="JNC24_001527"
     CDS             20907..21932
                     /gene="lpxD"
                     /locus_tag="JNC24_001527"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HAY9158303.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            22037..22492
                     /gene="fabZ"
                     /locus_tag="JNC24_001528"
     CDS             22037..22492
                     /gene="fabZ"
                     /locus_tag="JNC24_001528"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAY9158304.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            22496..23284
                     /gene="lpxA"
                     /locus_tag="JNC24_001529"
     CDS             22496..23284
                     /gene="lpxA"
                     /locus_tag="JNC24_001529"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HAY9158305.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            23284..24432
                     /gene="lpxB"
                     /locus_tag="JNC24_001530"
     CDS             23284..24432
                     /gene="lpxB"
                     /locus_tag="JNC24_001530"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HAY9158306.1"
                     /translation="MTEQRPLTIALVAGETSGDILGAGLIRALKEHVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNAARDVLGIPHDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            24429..25025
                     /gene="rnhB"
                     /locus_tag="JNC24_001531"
     CDS             24429..25025
                     /gene="rnhB"
                     /locus_tag="JNC24_001531"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HAY9158307.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALYEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEHGATEHHRRSFGPVKRALGLAS"
     gene            25062..28544
                     /gene="dnaE"
                     /locus_tag="JNC24_001532"
     CDS             25062..28544
                     /gene="dnaE"
                     /locus_tag="JNC24_001532"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HAY9158308.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLTLLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERVKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKPVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAKL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEAHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            28557..29516
                     /gene="accA"
                     /locus_tag="JNC24_001533"
     CDS             28557..29516
                     /gene="accA"
                     /locus_tag="JNC24_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HAY9158309.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAVSLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
BASE COUNT         7160 a         7120 c         8159 g         7109 t
ORIGIN      
        1 ttgaagatgc ggtagagaaa agaatattta ccgttgagca gctttatcat catttgcacg
       61 aggcgtgggg tcagcatgag aaaggttcgc tcttttcgct agtggttgaa aatgcctggg
      121 aaaaatcacg ctcaaatagt ttaagccgca gtacggaaga tcagtttttt atgtatttac
      181 gggtaaacac cctaaataaa ctggtaccct acgcggcaca acgatttatt gataatctgc
      241 ctgcgatttt cgccggaacg tttaatcatg cattattgga agatgccagc gaatgcagcg
      301 atcttcttaa gctatataaa aatgtcgctg taaaacatgt gtttagccat ccggatgtcg
      361 agcagcttga attgcagggc tatcgggtca ttagcggatt attagagatt tatcgtcctt
      421 tattaagcct gtcgttatca gactttactg aactggtaga aaaagaacgg gtgaaacgtt
      481 tccctattga gtcgcgctta ttccacaaac tctcgacgcg ccatcggctg gcctatgtcg
      541 aggctgtcag taaattaccg tcagattctc ctgagtttcc gctatgggaa tattattacc
      601 gttgccgcct actgcaggat tatatcagcg gtatgaccga cctctatgcg tgggatgaat
      661 accgacgtct gatggccgta gaacaataac caggcttttg taaagacgaa caataaattt
      721 ttaccttttg cagaaacttt agttcggaac ttcgcgctat aaaacgaatc tgaagaacac
      781 agcaattttg cgttatctgt taatcgagac tgaaatacat gaaaaaaacc acattagcac
      841 tgagtgcact ggctctgagt ttaggtttgg cgttatctcc gctctctgca acggcggctg
      901 agacttcttc agcaacgaca gcccagcaga tgccaagcct tgcaccgatg ctcgaaaagg
      961 tgatgccttc agtggtcagc attaacgtag aaggtagcac aaccgttaat acgccgcgta
     1021 tgccgcgtaa tttccagcag ttcttcggtg atgattctcc gttctgccag gaaggttcac
     1081 cgtttcagag ctctccgttc tgccaggggg gccagggcgg taatggcggc ggccagcaac
     1141 agaaattcat ggcgctgggt tccggcgtca tcattgatgc cgataaaggc tatgtcgtca
     1201 ccaacaacca tgttgttgat aacgcgacgg tgattaaagt ccagctgagc gatggtcgta
     1261 agttcgacgc gaagatggtt ggcaaagatc cgcgctctga tatcgcgctg atccaaatcc
     1321 agaacccgaa aaacctgacc gcaattaaga tggcggattc tgatgcactg cgcgtgggtg
     1381 attacaccgt agcgattggt aacccgtttg gtctgggcga gacggtaact tccgggattg
     1441 tctctgcgct ggggcgtagc ggcctgaatg ctgaaaacta cgaaaacttc atccagaccg
     1501 atgcagcgat caaccgtggt aactccggtg gtgcgctggt taacctgaac ggcgaactga
     1561 tcggtatcaa caccgcgatc ctcgcaccgg acggcggcaa catcggtatc ggttttgcta
     1621 tcccgagtaa catggtgaaa aacctgacct cgcagatggt ggaatacggc caggtgaaac
     1681 gcggtgagct ggggattatg gggactgagc tgaactccga actggccaaa gcgatgaaag
     1741 ttgacgccca gcgcggtgct ttcgtaagcc aggttctgcc taattcctcc gctgcaaaag
     1801 cgggcattaa agcgggtgat gtgatcacct cactgaacgg taagccgatc agcagctttg
     1861 ccgcactgcg tgctcaggtg ggtactatgc cggtgggcag caaactgacc ctgggcttac
     1921 tgcgcgacgg taagcaggtt aacgtgaacc tggaactgca gcagagcagc cagaatcagg
     1981 ttgattccag ctccatcttc aacggcattg aaggcgctga gatgagcaac aaaggcaaag
     2041 atcagggcgt ggtagtgaac aacgtgaaaa cgggcactcc ggctgcgcag atcggcctga
     2101 agaaaggtga tgtgattatt ggcgcgaacc agcaggcagt gaaaaacatc gctgaactgc
     2161 gtaaagtgct cgacagcaaa ccgtctgtgc tggcactgaa cattcagcgc ggcgacagca
     2221 ccatctacct gttaatgcag taatctccct caaccccttc ctgaatacgg gaaggggttc
     2281 tccttacaat ctgtgaactt cgccacaact ccatacatct tcatcatcct ttgggcattt
     2341 gcacaatgcc gtacgttacg tacttcctta tgctaagccg tgcataacgg aggacttatg
     2401 gctggctggc atcttgatac caaaatggcg caggatatcg tggcacgtac catgcgcatc
     2461 atcgatacca atatcaacgt aatggatgcc cgcgggcgaa ttatcggcag cggcgatcgt
     2521 gagcgtattg gtgaattgca cgaaggtgca ttgctggtac tttcacaggg acgagtcgtc
     2581 gatatcgatg acgcggtagc acgtcatctg cacggtgtgc ggcaggggat taatctaccg
     2641 ttacggctgg aaggtgaaat tgtcggcgta attggcctga caggtgaacc agagaatctg
     2701 cgtaaatatg gcgaactggt ctgcatgacg gctgaaatga tgctggaaca gtcgcggttg
     2761 atgcacctgt tggcgcagga tagccgtttg cgggaagaac tggtgatgaa cctgattcag
     2821 gctgaggaga atactcccgc acttactgaa tgggcgcaac ggctggggat tgatctcaat
     2881 caaccgcgag tggtggctat tgttgaggtc gacagcggtc agcttggcgt ggacagcgca
     2941 atggcggagt tacaacaact gcaaaatgcg ctgactacgc ccgagcgtaa taatctggtg
     3001 gcaattgttt cgctgaccga aatggtggtg ttgaaaccgg cgttgaactc ttttgggcgc
     3061 tgggatgcag aagatcatcg taagcgagtt gaacaactga ttacccgcat gaaagagtac
     3121 ggccagctgc gttttcgcgt ttcactgggc aactatttta ccggtcctgg cagtattgcc
     3181 cgatcctatc gtacggcgaa aacgacgatg gtggtgggta aacagcggat gccagaaagt
     3241 cgctgctatt tttatcagga tctgatgtta cctgtgttac tcgacagttt gcgtggcgac
     3301 tggcaggcca acgaactggc gcgaccgctg gcgcggctga aagcgatgga caataatggc
     3361 ttgctgcgac gaacgctggc ggcgtggttt cgccacaatg tgcaaccgct ggctacgtca
     3421 aaggcgctgt ttattcatcg taatactctg gagtatcggc ttaatcgtat atcggaactg
     3481 accgggcttg atttgggtaa ttttgatgac aggttgctgc tgtatgtggc gttacaactg
     3541 gatgaagagc ggtaggttat gcgttaaggt cgaggcggcg ctggctcatt cccctcagcc
     3601 cgaagggtag aggggaatgt ccgagttaac cgtcagttcg gcattattta cgcgttagtt
     3661 tttccagatc ggcttcaatc tcgctgatct tattggttac gactgactcc aggtgacgta
     3721 agtcgtcgag gatcttacgc ttaagatcaa cttcgctgcg gtcacgctgg cagatttgat
     3781 caagctcatc aatgatatac cgtagattcg ggctgatttc ctggacttct ttataaccct
     3841 gacccacacc atcagcgacg accgttttac gctgacgcgg atatttaaac ttcacgctct
     3901 tggcgaaaaa ctcgcctttg tctttctgga aatagatttt cagaatatcg ttgttggctt
     3961 cctgccggag gctgtaacga tcaatttctt caggattggt aatacccaga cttttcagat
     4021 tgtcgtacat agcgttacct caaaatgagt cagtaaattg tgcttatttt agcatttggc
     4081 atggcccgcc cggctcgact gtgatcgcag aaagcctttt tcttccagac ttctatcatg
     4141 gcgtaaaaat caaaaaatta cctgctttat tgtggtgata aaattcacgt tctacaccag
     4201 ttccagcatc gttacttccg ggcggcagtt cagacgcaaa ccatacaaac tgcccacgcc
     4261 acgggttgtg tagatgtgtc gttcgccaaa ggcatttaat ccggcgacgt aacgtttatc
     4321 ttcgacaggg gcaaaaggtt caccaaccaa cggtacgcgc agttgcccgc cgtgagtatg
     4381 gccgcacagc atcagatccc agggttcatc acgcatgact tctttgctgt cgggattatg
     4441 tgccagcatc agtcttggca gatttgcttc gctggcggga ggtggtttgc attgtccggc
     4501 ccataagtca ccggtgccta ccagttcgaa ttgcctgttc ggcgtggcga tcaccgtagc
     4561 ctggttaaac aacaccgtga tgcccactga tttcaacgtc tcgccaatta agtgattttt
     4621 ttctgtacca acagggcgat cgtggttgcc gaagcaggca aacgtcggcg cacattcggc
     4681 aaggggggag aggacatcac taaacgccga aaaattcagc ggcatatcaa ataatacgta
     4741 atcgccgccc agtaatatca aatcgggctt ttgttctatg ccaagagcaa tcgcgtcaga
     4801 aatcaggctt aaaggaacaa aacgagagta atggagatcg gccagaaaaa gaattttgaa
     4861 tggtgctgcg ttgtctttaa aaaaggcgag gcggtgacgg attaattcaa accagcctag
     4921 ctcccagtaa tgcatataac cgaaacctga gctcgtggct atcgtcgcgg cagtagcctg
     4981 caacaatcgg cggcgtgaaa tcatcgctca tccctgcaaa aaaaatcggg cagcgtcgtg
     5041 ctgcccgtgt gcatactttt agtcgatggt acgcagcagt tcgttaatgc cgactttgcc
     5101 gcgagttttc gcgtcaactt tcttaacgat aaccgcacag tagaggctgt atttgccatc
     5161 ttttgacggc agattacctg aaacaaccac agaccccgcc ggaacgcgac cgtagtggat
     5221 ttcgccggtt tcacggtcgt aaatacgggt gctctgacca atgtatacgc ccatggaaat
     5281 gacggaacct tcttcgacaa tcaccccttc aaccacttca gagcgtgcgc caatgaagca
     5341 gttatcttca atgatggttg ggttagcctg cagcggttcc agcacgccgc cgatgcccac
     5401 gccaccggaa aggtggacgt ttttaccaat ctgcgcacaa gaaccgacgg tcgcccaggt
     5461 atcaaccatg gtgccttcat caacatatgc gccgatgttg acgtaagacg gcatcagcac
     5521 ggtgttacgg gcaataaacg caccctgacg taccgccgct ggtggcacaa cgcggaagcc
     5581 ttctttctgg aaacgtgctt cgtcgtagtc ggcgaatttc atcggcactt tgtcgaagta
     5641 gcggctttct gccccttcga tcacctgatt atcattaata cggaaagaga gcagcaccgc
     5701 ttttttcaac cactgatgcg tcacccactg accgtcaatt ttttccgcta cacgcagtgc
     5761 accggaatcc agcagggcga tcacctgatt taccgcttcg cgggtaacgg tgtctgcgtt
     5821 ggctggcgtg atctcggcac ggcgttcaaa agcggtttca ataatgttct gtaactgctg
     5881 cattgttaaa ctcttttcat atcagtaaac acatcaccct ttatcgtttg gattgagggc
     5941 ctctgtcaac cgctgatgca cttcctgctg caactcgtta ttaagtgcac gccggtcagc
     6001 ggtggcaatt atgaataaat cttctactcg ctcgccaatg gttgtaattc gggcaccatg
     6061 aagcgaaatt cccagatcgg caaaaatttt cccgactctc gccagcagtc ctggctggtc
     6121 gagcgcgata agctcaagga acgatttgcg gtcggtatgg gttggcaaaa acgtcacttc
     6181 ggtttcaaca gtaaagtgac gtagtttagc cggttgacga cgcggctgtg gtggctgcca
     6241 gctactttgt gtcagtacct gctccagacc aaaccgaata acctcatgac gatctgcgga
     6301 tagcgggcta ccatcgggtt ccagcacgat gaaggtatcc atcgccatac cgtcgcgagt
     6361 ggtgaaaatt tgtgcgtcgt gaacacttaa attgcggcgg tctaattcgg cacagacggc
     6421 ggcaaacaga taggggcggt ccgggctcca gataaaaatc tcggtgcctc cacgcgtagc
     6481 ctgcgggcta agcaatacca gcggtttgct taaatcatgc tgtaataaat ggcgggcatg
     6541 ccaggccagt tgatttgggc tatggcggac aaaatagtta gcacgacagc gtgaccaaat
     6601 ttggtgcagc gcctcttcgt cgatgttatc catgcgcagt agtgccagtg cctggagttg
     6661 gtgatggcga acccgttcgc gcatatccgg cgtgttttgc attccgcgtc gtagctgctt
     6721 ttcggtggca aaatagagtt cgcgcaacag actttgcttc cagctattcc acagcgtttc
     6781 gttggtggcg caaatgtcag ccactgtcag gcataccaga tagtgcagac gattttccgt
     6841 ttgaacttct tcggcaaact gcttgatgac ttcagggtcc tgaatatcgc ggcgttgtgc
     6901 tgtcaccgac atcagcaagt gctggcgaac cagccaggca accagctgtg tttcgcgtga
     6961 gttcagcccg tggagttctg caaaatgcac tacatcctga gcaccgagaa tggagtggtc
     7021 gccgccgcgt cctttggcga tatcgtgaaa cagcgcggcg atgaaaatca gctcagttga
     7081 cggcaggcgc ggccagacgt ccacacacaa cggatggcgc tggcgcgttt cttcactggc
     7141 aaaactctcc agtttcagca tcacgcggat agtatgttca tccaccgtgt aggcgtggaa
     7201 cagatcaaac tgcatctgcc cgacgatatg cgaccattgc ggcatatacg cgccgagcac
     7261 gctatggcga tgcattggca atagcccgcg ccgcaccgct ccggggtgac gcagaatgct
     7321 caaaaacagt tttcgtgctt ccggaatatt acacagcggt tgttgcagat ggcgacgggc
     7381 atggcgtaac tggcgcagcg tggtggagta aatgccggtg atcgcactgt tgcgcaccat
     7441 ggtgtagaac atacgcaaga tggcttccgg ctggcgcata aatagtgttt catcacgcag
     7501 gtcgattagc gtaccgcgta gctgaaactc atcgtcgatt ggacgtggtt tttcgtcggc
     7561 gggaagggcg aggatggctt catcgaacag ttgcagcagc atctggttga gttcactgac
     7621 gcggcgtgta acgcggaagt aatccttcat catccgctcg accggttcgt tgccttcacc
     7681 actgtaattc agacgctgag cgacgctaag ttggcgatcg aataacaggc gattatcgta
     7741 acggctgacg accagatgca gggcaaagcg aatacgccac agtatatgca gacattcgtt
     7801 taattccgcc cgctccgctg aggttaagaa gccaaaccca accatttcat ccagcgatgt
     7861 tgcgccaaaa tgacggcggg ccacccattg cagagtgtgg atatcgcgca agccgccagg
     7921 gctacttttg atgtctggtt caagattgta gctggtgccg tggtaacgct gatggcgctg
     7981 gttctgttct tcaactttcg ccgcgtagaa cttgtcggaa ggccagaatc cttcgctaaa
     8041 aatatgtttt tgcagttcga ggaagagcgc aacatcgcca attaataagc gggattcgat
     8101 taaattggtg gcgacggtta aatccgataa cccttccagc atgcactctt caagcgtgcg
     8161 cacactatga ccgacttcca gctttacatc ccagagcagc gttaacagct cgcccacttt
     8221 ttgcgcctga tcgtccggga gctttttacg gcttaaaatc agtaaatcga tgtcagaaag
     8281 tggatgcagc tcgccacgac cgtagccacc gacggcgacc aatgccaggt cggcaatctg
     8341 gctgaatccc gcttcaatcc ataatcgttg caggagctgg tcgataaact cggtgcgcgc
     8401 ctcaatcaac tgttctgcag agatcccatt gtcaaaggca tcacccagcc aacgctggaa
     8461 agtatcgata tgggctttta tcccaccgac ggttaattca tcacggggcc agacgcatgg
     8521 attttgcggt tgaccgggca gggtggggag agcggtgttt gcgtactgtt ctggaagggt
     8581 attcattgtg cgccacccat aagattaaat tatcgcatta aaaaagccgg cattcgccgg
     8641 cttcatctta ttcgtcgtgc gagattatcg ccgggatggt gtcatccttg cgtagcgtca
     8701 gaatttcgca gccgttatca gtcaccacaa tagtatgctc atattgtgca gacaagctgc
     8761 gatctttggt ttttaccgtc cagccatctt tcatggtgcg gatctctttt ttacccgcgt
     8821 tgaccattgg ctcgatggtg aacgtcatcc caggtttcag tacgacgttg gtttcacggg
     8881 agtcatagtg cagcacctgc ggttcttcat ggaagccgcg accaataccg tgtccgcaat
     8941 attcacgaac gacggagaag ccttctgctt cgataaactt ctgaatcgcc gcaccgattt
     9001 cgcgcaggtt aatgcctggt ttgaccatgc gcagcgccag gtacaggctt tcttgcgtga
     9061 tgcggcacag acgttcgccc atgatggtcg gcttaccgac gataaacatt ttcgaggtat
     9121 cgccgtggaa accatctttg attacggtga catcaatgtt aacgatatcg ccatctttca
     9181 gcagcttcgc atcgtccggg ataccgtggc acaccacttc attaatagag atgcaaacgg
     9241 atttcggata gccgtgatag ccgaggcagg cagaaaccgc gtgttgttca ttaacaatgt
     9301 aatcattaca gatgcgatcc agctcgccgg tgctgacgcc cggtttaaca tacggttcga
     9361 tcatctccag cacttcggca gccagtcggc cagcgacgcg cattttttcg atatcttctg
     9421 gggtcttgat tgagatagcc attaattctg tccatcagcg tcggtgatac cgacaatata
     9481 tatgtaagtg ccgtcaatgg tatcacaccc gggcaaattg agaatcattc tgaatttcgc
     9541 caaacgtgcc actgaacgtt ttctataata gaaaattcaa cgtctgatgc tgtacacagc
     9601 gccaacaatt attggtgtcc atgacgtatt tgtggtataa agcgcgccgg acttccgatc
     9661 catttcgtat acacagactg gacggaagcg acaatctcac tttgtgtaac aacacacacg
     9721 tatcggcaca tattccgggg tgccctttgg ggtcggtaat atgggatacg tggaggcata
     9781 accccaactt ttatatagag gttttaatca tggcaactgt ttccatgcgc gacatgctca
     9841 aggctggtgt tcacttcggt caccagaccc gttactggaa cccgaaaatg aagccgttca
     9901 tcttcggtgc gcgtaacaaa gttcacatca tcaaccttga gaaaactgta ccgatgttca
     9961 acgaagctct ggctgaactg aacaagattg cttctcgcaa aggtaaaatc cttttcgttg
    10021 gtactaaacg cgctgcaagc gaagcggtga aagacgctgc tctgagctgc gaccagttct
    10081 tcgtgaacca tcgctggctg ggcggtatgc tgactaactg gaaaaccgtt cgtcagtcca
    10141 tcaaacgtct gaaagacctg gaaactcagt ctcaggacgg tactttcgaa aagctgacca
    10201 agaaagaagc gctgatgcgc actcgtgagc tggagaaact ggaaaacagc ctgggcggta
    10261 tcaaagacat gggcggtctg ccggacgctc tgtttgtaat cgatgctgac cacgaacaca
    10321 ttgctatcaa agaagcaaac aacctgggta ttccggtatt tgctatcgtt gataccaact
    10381 ctgatccgga cggtgttgac ttcgttatcc cgggtaacga cgacgcaatc cgtgctgtga
    10441 ccctgtacct gggcgctgtt gctgcaaccg tacgtgaagg ccgttctcag gatctggctt
    10501 cccaggcgga agaaagcttc gtagaagctg agtaataagg cttgataact cccccaaaat
    10561 agttcgagtt gcagaaaggc ggcaagctcg agaattcccg ggagcttaca tcagtaagtg
    10621 accgggatga gcgagcgaag acaacgcatc tgcggcgcga aatatgaagg gggagagccc
    10681 ttatagacca ggtagtacac gtttggttag ggggcctgca tatggccccc tttttcactt
    10741 ttatatctgt gcggtttaat gccgggcaga tcacatctcc gaggatttta gaatggctga
    10801 aattaccgca tccctggtaa aagagctgcg tgagcgtact ggcgcaggca tgatggattg
    10861 caaaaaagca ctgactgaag ctaacggcga catcgagctg gcaatcgaaa acatgcgtaa
    10921 gtccggtgct attaaagcag cgaaaaaagc aggcaacgtt gctgctgacg gcgtgatcaa
    10981 aaccaaaatc gacggcaact acggcatcat tctggaagtt aactgccaga ctgacttcgt
    11041 tgcaaaagac gctggtttcc aggcgttcgc agacaaagtt ctggacgcag ctgttgctgg
    11101 caaaatcact gacgttgaag ttctgaaagc acagttcgaa gaagaacgtg ttgcgctggt
    11161 agcgaaaatt ggtgagaaca tcaacattcg ccgcgttgct gcgctggaag gcgacgttct
    11221 gggttcttat cagcacggtg cgcgtatcgg cgttctggtt gctgctaaag gcgctgacga
    11281 agagctggtt aaacacatcg ctatgcacgt tgctgcaagc aagccagaat tcatcaaacc
    11341 agaagacgta tccgctgaag tggtagaaaa agaataccag gtacagctgg atatcgcgat
    11401 gcagtctggt aagccgaaag aaatcgcaga gaaaatggtt gaaggccgca tgaagaaatt
    11461 caccggcgaa gtttctctga ccggtcagcc gttcgttatg gaaccaagca aaactgttgg
    11521 tcagctgctg aaagagcata acgctgaagt gactggcttc atccgcttcg aagtggggga
    11581 aggcatcgag aaagttgaga ctgactttgc agcagaagtt gctgcgatgt ccaagcagtc
    11641 ttaattatcg aaaaggagcc gcctgagggc ggcttctttt tgtgcccatc ttgtaaattc
    11701 agctaaccct tgtggggctg cgctgaaaag cgacgtacaa tgtcgctagt attaattcat
    11761 ttcaatcgtt gacagtctca ggaaagaaac atggctacca atgcaaaacc cgtctataaa
    11821 cgcattctgc ttaagttgag tggcgaagct ctgcagggca ctgaaggctt cggtattgat
    11881 gcaagcatac tggatcgtat ggctcaggaa atcaaagaac tggttgaact gggtattcag
    11941 gttggtgtgg tgattggtgg gggtaacctg ttccgtggcg ctggtctggc gaaagcgggt
    12001 atgaaccgcg ttgtgggcga ccacatgggg atgctggcga ccgtaatgaa cggcctggca
    12061 atgcgtgatg cactgcaccg cgcctatgtg aacgctcgtc tgatgtccgc tattccattg
    12121 aatggcgtgt gcgacagcta cagctgggca gaagctatca gcctgttgcg caacaaccgt
    12181 gtggtgatcc tctccgccgg tacaggtaac ccgttcttta ccaccgactc agcagcttgc
    12241 ctgcgtggta tcgaaattga agccgatgtg gtgctgaaag caaccaaagt tgacggcgtg
    12301 tttaccgctg atccggcgaa agatccaacc gcaaccatgt acgagcaact gacttacagc
    12361 gaagtgctgg aaaaagagct gaaagtcatg gacctggcgg ccttcacgct ggctcgtgac
    12421 cataaattac cgattcgtgt tttcaatatg aacaaaccgg gtgcgctgcg ccgtgtggta
    12481 atgggtgaaa aagaagggac tttaatcacg gaataattcc cgtgatggat aaataagggt
    12541 aagattccgc gtaagtatcg cgggggcgta agtctggtta taaggcgtta ttgttgcagg
    12601 cagtttggtc acggccagcg cgcagcaacc ggagcgtaca aaagtacgtg aggatggcga
    12661 gcactgctcg gggccaaaat ggcaaataaa atagcctaat aatccagacg attacccgta
    12721 atatgtttaa tcagggctat acttagcaca cttccactgt gtgtgactgt ctggtctgac
    12781 tgagacaagt tttcaaggat tcgtaacgtg attagcgata tcagaaaaga tgctgaagta
    12841 cgcatggaca aatgcgtaga agcgttcaaa acccaaatca gcaaaatacg cacgggtcgt
    12901 gcttctccca gcctgctgga tggcattgtc gtggaatatt acggcacgcc gacgccgctg
    12961 cgtcagctgg caagcgtaac ggtagaagat tcccgtactc tgaaaatcaa cgtgtttgat
    13021 cgttcaatgt ctccggccgt tgaaaaagcg attatggcgt ccgatcttgg cctgaacccg
    13081 aactctgcgg gtagcgacat ccgtgttccg ctgccgccgc tgacggaaga acgtcgtaaa
    13141 gatctgacca aaatcgttcg tggtgaagca gaacaagcgc gtgttgcagt acgtaacgtg
    13201 cgtcgtgacg cgaacgacaa agtgaaagca ctgttgaaag ataaagagat cagcgaagac
    13261 gacgatcgcc gttctcagga cgatgtacag aaactgactg atgctgcaat caagaaaatt
    13321 gaagcggcgc tggcagacaa agaagcagaa ctgatgcagt tctgatttct tgaacgacaa
    13381 aaacgccgct cagtagatcc ttgcggatcg gctggcggcg ttttgctttt tattctgtct
    13441 caactctgga tgtttcatga agcaactcac cattctgggc tcgaccggct cgattggttg
    13501 cagcacgctg gacgtggtgc gccataatcc cgaacacttc cgcgtagttg cgctggtggc
    13561 aggcaaaaat gtcactcgca tggtagaaca gtgcctggaa ttctctcccc gctatgccgt
    13621 aatggacgat gaagcgagtg cgaaacttct taaaacgatg ctacagcaac agggtagccg
    13681 caccgaagtc ttaagtgggc aacaagccgc ttgcgatatg gcagcgcttg aggatgttga
    13741 tcaggtgatg gcagccattg ttggcgctgc tgggctgtta cctacgcttg ctgcgatccg
    13801 cgcgggtaaa accattttgc tggccaataa agaatcactg gttacctgcg gacgtctgtt
    13861 tatggacgcc gtaaagcaga gcaaagcgca attgttaccg gtcgatagcg aacataacgc
    13921 catttttcag agtttaccgc aacctatcca gcataatctg ggatacgctg accttgagca
    13981 aaatggcgtg gtgtccattt tacttaccgg gtctggtggc cctttccgtg agacgccatt
    14041 gcgcgatttg gcaacaatga cgcccgatca agcctgccgt catccgaact ggtcgatggg
    14101 acgtaaaatt tctgtcgatt cggctaccat gatgaacaaa ggtctggaat acattgaagc
    14161 gcgttggctg tttaacgcca gcgccagcca gatggaagtg ctgattcacc cgcagtcagt
    14221 gattcactca atggtgcgct atcaggacgg cagtgttctg gcgcagctgg gggaaccgga
    14281 tatgcgtacg ccaattgccc acaccatggc atggccgaat cgcgtgaact ctggcgtgaa
    14341 gccgctcgat ttttgcaaac taagtgcgtt gacatttgcc gcaccggatt atgatcgtta
    14401 tccatgcctg aaactggcga tggaggcgtt cgaacaaggt caggcagcga cgacagcatt
    14461 gaatgccgca aacgaaatca ccgttgctgc ttttcttgcg caacaaatcc gctttacgga
    14521 tatcgctgcg ttgaatttat ctgtactgga aaaaatggat atgcgcgaac cacaatgtgt
    14581 ggacgatgtg ttatctgttg atgcgaacgc gcgtgaagtc gccagaaaag aggtgatgcg
    14641 tctcgcaagc tgaggataat ccggctacag agagtcgcgc tatttgttag cgtagggctt
    14701 cagtgatata gtctgcgcca tctgatcgta agtagttggc tttataaggt caggtatgcc
    14761 gtggttttac acggcttttt tttgtatagg cttcagtatt cctgagtacc gtaaaccctg
    14821 tcagggaata aaaaacgcgt gatgttgtct gctactcaac cacttagcga aaaattgcca
    14881 gcgcatggct gccgtcatgt tgcgatcatt atggacggca atggtcgctg ggcaaaaaag
    14941 caagggagga ttcgtgcctt tgggcataaa gccggggcaa aatccgtccg ccgggctgtc
    15001 tcttttgcgg ccaacaacgg tattgaggcg ttaacgctgt atgcctttag tagtgaaaac
    15061 tggaaccgac cagcgcagga agtcagtgcg ttaatggaac tgtttgtgtg ggcgctcgat
    15121 agcgaagtaa aaagtctgca ccgacataac gtgcgtctgc gtattattgg cgataccagt
    15181 cgctttaact cgcgtttgca agaacgtatt cgtaaatctg aagcgctaac agctgggaat
    15241 accggtctga cgctgaatat tgcggcgaac tacggtggac gttgggatat agtccaggga
    15301 gtcaggcaac tggctgaaaa ggtgcagcaa ggaaacctgc aaccagatca gatagatgaa
    15361 gagatgctaa accagcatgt ctgtatgcat gaactggccc ctgtagattt agtaattagg
    15421 actggggggg agcatcgcat tagtaacttt ttgctttggc aaattgccta tgccgaactt
    15481 tactttacag atgttctctg gcctgatttc gatgaacaag actttgaagg ggcgttaaat
    15541 gcctttgcta atcgagagcg tcgtttcggc ggcaccgagc ccggtgatga aacagcctga
    15601 tggggggcgc ttttgctgaa gtatcgcctg atatctgctt ttgtgttaat acccgtcgtc
    15661 atcgcggcgt tgtttctgtt gccgccggtg gggttcgcca tcgtaacgct ggtggtctgc
    15721 atgctggcag cgtgggaatg gggacagctt agcggtttta ccactcgttc gcagcgagta
    15781 tggttggcgg tgttatgcgg gttattgttg gcgctgatgc tttttctgtt gccggaatat
    15841 caccgaaata ttcatcaacc gctggttgaa atctcacttt gggcttcgtt gggttggtgg
    15901 attgtcgcgc tattgctggt gctgttttac ccaggttccg cagcaatctg gcgtaactct
    15961 aaaacattgc gccttatttt tggcgtgcta accattgttc ccttcttctg gggcatgctg
    16021 gcgttacggg cctggcacta tgacgagaat cattacagtg gcgcaatatg gctgctctat
    16081 gtcatgatcc tggtatgggg cgctgactcc ggcgcatata tgtttggcaa attgtttggt
    16141 aaacataagc tggcaccgaa ggtttctccg ggtaaaacct ggcaaggctt tataggtgga
    16201 ctagcgaccg cagcggtaat ctcatggggt tatggcatgt gggcgaatct cgacgtcgct
    16261 cccgtcacct tactcatttg ctctattgtc gcagcgttag cctcagtgct cggcgatctg
    16321 accgagagta tgtttaagcg tgaagcagga attaaggaca gcggtcattt aattccagga
    16381 cacggtggta ttttagatcg tattgatagc ctgacggctg cggtaccggt ctttgcttgc
    16441 ttgttgttac tggtattcag gacgctttaa cggaaggtaa tatgctgagt tttctctggg
    16501 atttggcttc gttcatcgtt gcactgggtg tacttatcac cgtgcatgaa tttggtcatt
    16561 tctgggttgc ccggcgttgt ggtgttcgcg ttgagcgttt ctcaataggg tttggtaagg
    16621 cgctctggcg gcgaactgat aagctcggca ccgaatatgt tatggccctg attccgctgg
    16681 gcggttatgt caaaatgctg gatgagcgcg cagaaccggt cgttccggaa ctccgccacc
    16741 atgccttcaa taataaatct gttggccaac gagcggcgat tattgccgca ggtccggttg
    16801 caaacttcat ttttgctatc tttgcctact ggctggtttt tattattggt gtgcctggcg
    16861 tacgtccggt ggttggtgaa atagcagcca attcgatagc tgcggaagca caaattgcac
    16921 caggtacgga actaaaagcc gtagatggta tcgaaacgcc tgattgggat gccgtgcgtt
    16981 tgcagttggt cgataaaatt ggcgatgaaa gcaccaccat tacagtagcg ccatttggca
    17041 gcgaccaacg gcgggatgta aagctcgatt tacgtcactg ggcgtttgag cctgataaag
    17101 aagatccggt atcttcgctg gggattcgtc ctcgtgggcc gcaaattgaa cctgtactgg
    17161 aaaatgtgca gccaaactcg gcggcaagca aggcaggttt gcaagcaggc gacaggatcg
    17221 ttaaagtcga tggtcagccc ttaacgcagt gggtgacctt tgtgatgctt gtccgggata
    17281 acccgggtaa atccttagcg ttagaaatcg aaaggcaggg gagtcccttg tctttgacat
    17341 taatcccgga gagtaaaccg ggtaatggta aagcgattgg ttttgtcggt attgagccga
    17401 aagtcattcc tttgccagat gagtataaag ttgtacgcca gtatgggccg ttcaacgcca
    17461 tcgtcgaagc cacggacaaa acgtggcagc tgatgaagct gacggtcagt atgctgggaa
    17521 aattgatcac cggtgatgtg aaactgaaca acctcagtgg gccgatctct atcgccaagg
    17581 gggctgggat gacagcggaa ctcggggtag tttattacct gccgtttctt gcgcttatta
    17641 gcgtgaactt agggataatt aacctgtttc cgttgcccgt acttgacggg gggcatctgc
    17701 tgttccttgc gatcgaaaag atcaagggcg gaccggtatc cgagcgggtt caagactttt
    17761 gttatcgcat tggctcgatt ctgctggtgc tgttaatggg gcttgcactt ttcaatgatt
    17821 tctctcggtt atgagagtta gttaggaaga acgcataata acgatggcga tgaaaaagtt
    17881 gctcatagcg tcgctgctgt ttagcagcgc caccgtatac ggtgctgaag ggttcgtagt
    17941 gaaagatatt catttcgaag gccttcagcg tgtcgccgtt ggtgcggcac tcctcagtat
    18001 gccggtgcgc acaggcgaca cggttaatga tgaagatatc agtaatacca ttcgcgctct
    18061 gtttgctacc ggcaactttg aggatgttcg cgtccttcgt gatggtgata cccttctggt
    18121 tcaggtaaaa gaacgtccga ccattgccag cattactttc tccggtaaca aatcggtgaa
    18181 agatgacatg ctgaagcaaa acctcgaggc ttctggtgtg cgtgtgggcg aatccctcga
    18241 tcgcaccacc attgccgata tcgagaaagg tctggaagac ttctactaca gcgtcggtaa
    18301 atatagcgcc agcgtaaaag ctgtcgtgac cccgctgccg cgcaaccgtg ttgacctaaa
    18361 actggtgttc caggaaggtg tgtcagctga aatccagcaa attaacattg ttggtaacca
    18421 tgctttcacc accgacgaac tgatctctca tttccaactg cgtgacgaag tgccgtggtg
    18481 gaacgtggta ggcgatcgta aataccagaa acagaaactg gcgggcgacc ttgaaaccct
    18541 gcgcagctac tatctggatc gcggttatgc ccgtttcaac atcgactcta cccaggtcag
    18601 tctgacgcca gataaaaaag gtatttacgt cacggtgaac attaccgaag gcgatcagta
    18661 caagctttct ggcgttgaag tgagcggcaa ccttgccggg cactccgctg aaattgagca
    18721 gctgactaag atcgagccgg gcgagctgta taacggcacc aaagtgacca agatggaaga
    18781 tgacatcaaa aagcttctcg gtcgctatgg ttatgcctat ccgcgcgtac agtcgatgcc
    18841 tgaaattaac gatgccgaca aaaccgttaa attacgcgtg aacgttgatg cgggtaaccg
    18901 tttctacgtg cgtaagatcc gttttgaagg taacgatacc tcgaaagatg ccgtcctgcg
    18961 tcgcgaaatg cgtcagatgg aaggtgcatg gctgggaagc gatctggtcg atcagggtaa
    19021 ggagcgtctg aatcgtctgg gcttctttga aactgtcgat accgataccc aacgtgttcc
    19081 gggtagcccg gaccaggtag atgtagtcta caaggtgaaa gaacgtaaca ccggtagctt
    19141 caacttcggg attggttacg gtactgaaag tggcgtgagc ttccaggctg gcgtgcaaca
    19201 ggataactgg ttaggtacag gttatgctgt tggtatcaac gggaccaaaa acgattacca
    19261 gacctatgct gaattgtcgg taaccaaccc gtacttcact gtagatggtg taagcctcgg
    19321 tggtcgtctc ttctataatg acttccaggc agatgacgcc gacctgtccg actataccaa
    19381 caagagttat ggtacagacg tgacgttggg cttcccgatc aacgaatata actcgctgcg
    19441 tgcaggtctg ggttatgtac ataactccct gtccaacatg cagcctcagg ttgcgatgtg
    19501 gcgttatctg tactctatgg gtgaacatcc gagcacctct gatcaggata acagcttcaa
    19561 aacggacgac ttcacgttca actatggttg gacctataac aagcttgacc gtggttactt
    19621 cccgacagat ggttcacgtg tcaacctgac cggtaaagtg accattcctg gatcggataa
    19681 cgaatactac aaagtgacgt tagacacggc gacttatgtg ccgatcgatg acgatcacaa
    19741 atgggttgtt ctggggcgta cccgctgggg ttatggtgat ggtttaggcg gcaaagagat
    19801 gccgttctac gagaacttct atgccggtgg ttccagcacc gtgcgtggct tccagtccaa
    19861 tacaattggt ccgaaagcag tttacttccc gcatcaggcc agtaattatg atccggacta
    19921 tgattacgaa tgtgcgactc aggacggcgc gaaagacctg tgtaaatcgg atgatgctgt
    19981 aggcggtaac gccatggcgg ttgccagcct cgagttcatc accccgacgc cgtttattag
    20041 cgataagtat gctaactcgg ttcgtacttc cttcttctgg gatatgggta ccgtttggga
    20101 tacaaactgg gattccagcc aatattctgg atatccggac tatagtgatc caagcaatat
    20161 ccgtatgtct gcgggtatcg cattacaatg gatgtcccca ttggggccgt tggtgttctc
    20221 ctacgcccag ccgttcaaaa agtacgatgg agacaaggca gaacagttcc agtttaacat
    20281 cggtaaaacc tggtaagtgt tctccacaaa ggaatgtagt ggtagtgtag cgatgacttt
    20341 aggcgatcaa tataagatcg ccgggccacg caaagaactg caccctccgg tgcaaatggg
    20401 atggtaagga gtttattgtg aaaaagtggt tattagcggc aggtctcggt ttagcactgg
    20461 caacttctgc tcaggcggct gacaaaattg caatcgtcaa tatgggcagc ctgttccagc
    20521 aggtagcgca gaaaaccggt gtttctaaca cgctggaaaa tgagttcaaa ggccgtgcca
    20581 gcgaactgca gcgtatggaa accgatctgc aggctaaaat gaaaaagctg cagtccatga
    20641 aagcgggcag cgatcgcact aagctggaaa aagacgtgat ggctcagcgc cagacttttg
    20701 ctcagaaagc gcaggctttt gagcaggatc gcgcacgtcg ttccaacgaa gaacgcggca
    20761 aactggttac tcgtatccag actgctgtga aatccgttgc caacagccag gatatcgatc
    20821 tggttgttga tgcaaacgcc gttgcttaca acagcagcga tgtaaaagac atcactgccg
    20881 acgtactgaa acaggttaaa taagtaatgc cttcaattcg actggctgat ttagcgcagc
    20941 agttggatgc agaactacac ggtgatggcg atatcgtcat caccggcgtt gcgtccatgc
    21001 aatctgcaca aacaggtcac attacgttca tggttaaccc aaaataccgt gagcatttag
    21061 gcttgtgcca ggcgtccgcg gttgtcatga cccaggacga tcttcctttc gcgaaaagtg
    21121 ccgcgctggt agtgaagaat ccctacctga cttacgcgcg catggcgcaa attttagata
    21181 ccacgccgca gcccgcgcag aacattgcac ccagtgcggt gatcgacgcg acggcgaagc
    21241 tgggtaacaa cgtatcgatt ggcgctaacg cggtgattga gtccggcgtt gaactgggcg
    21301 ataacgtgat tatcggtgcc ggttgcttcg taggtaaaaa cagcaaaatc ggtgcaggtt
    21361 cgcgtctctg ggcgaacgta accatttacc atgagatcca gatcggtcag aattgcctga
    21421 tccagtccgg aacagtggta ggcgcagacg gctttggtta tgccaacgat cgtggtaact
    21481 gggtgaagat cccacagatt ggtcgcgtaa ttattggcga tcgcgtggag atcggtgcct
    21541 gcacaaccat cgatcgcggc gcgctggatg acactgttat tggcaatggc gtgatcattg
    21601 ataaccagtg ccagattgca cataacgtcg tgattggcga caatacggcg gttgccggtg
    21661 gcgtcattat ggcgggcagc ctgaaaattg gtcgttactg catgatcggc ggagccagcg
    21721 taatcaacgg gcatatggaa atatgcgaca aagtgacggt tacgggcatg ggtatggtga
    21781 tgcgtcccat cactgaacca ggcgtctatt cctcaggcat tccgctgcaa cccaacaaag
    21841 tctggcgcaa aaccgctgca ctggtgatga acattgatga catgagcaag cgtctgaaat
    21901 cgcttgagcg caaggttaat caacaagact aacgttccat cttttgttcg ccaaacttta
    21961 cggcctgtct cattcttacg attgcggcag gccgtgttat tattgtcgtt tcttatattt
    22021 tgacaggaag agtatcttga ctactaacac tcatactctg cagattgaag agattttaga
    22081 acttctgccg caccgtttcc cgttcttact ggtggatcgc gtgctggatt ttgaagaagg
    22141 tcgttttctg cgcgcagtaa aaaatgtctc tgtcaatgag ccattcttcc agggccattt
    22201 ccctggaaaa ccgattttcc cgggtgtgct gattctggaa gcaatggcac aggcaacagg
    22261 tattctggcg tttaaaagcg taggaaaact ggaaccgggt gagctgtact acttcgctgg
    22321 tattgacgaa gcgcgcttca agcgcccggt cgtgcctggc gatcaaatga tcatggaagt
    22381 cactttcgaa aaaacgcgcc gcggcctgac ccgttttaaa ggggttgctc tggtcgatgg
    22441 taaagtagtt tgcgaagcaa cgatgatgtg tgctcgtagc cgggaggcct gatacgtgat
    22501 tgataaatcc gcctttgtgc atccaaccgc cattgtggaa gagggcgcgt caattggcgc
    22561 gaacgcacac attggtcctt tttgtatcgt tggaccccat gtcgaaattg gtgagggtac
    22621 cgtactgaaa tctcacgttg tcgtgaatgg tcatactaaa attggccgcg ataatgagat
    22681 ttatcagttc gcctccatcg gcgaagttaa ccaggatctg aaatatgctg gcgaaccgac
    22741 ccgtgtggaa atcggcgatc gtaaccgcat tcgcgaaagc gtcaccattc atcgtggcac
    22801 agtccagggc ggtggattga cgaaggtggg cagcgacaac ttactgatga tcaacgcgca
    22861 cattgcgcac gattgtacgg taggtaaccg ctgtattctc gccaacaacg caacgctggc
    22921 gggtcacgta tcggttgacg acttcgcgat catcggcggc atgaccgcag tccatcagtt
    22981 ctgcatcatt ggtgcgcacg tgatggttgg cggctgctcc ggtgtggcgc aggacgtccc
    23041 tccttatgtc attgcgcagg gtaaccacgc aacgccgttc ggtgtcaata tcgaagggct
    23101 gaagcgccgc ggattcagcc gtgaggcgat taccgctatc cgcaatgcgt ataagctgat
    23161 ttatcgtagc ggtaaaacgc tcgatgaagt gaaaccggaa attgctgaac tggcggaaac
    23221 atatccggaa gtgaaagcct ttaccgattt ctttgcacgc tcaacgcgcg gtctgattcg
    23281 ttaatgactg aacagcgtcc attaacgatt gccctggtcg ccggagaaac ctccggcgat
    23341 atcctggggg ccggtttaat ccgcgctctg aaagaacatg tgcccaacgc ccgctttgtt
    23401 ggtgttgccg ggccacgaat gcaggctgaa ggctgcgaag cttggtacga aatggaagaa
    23461 ctggcggtaa tgggcattgt tgaagtgctc ggtcgtctgc gtcgcttact gcatattcgt
    23521 gccgatctga caaagcgttt tggcgaactg aagccagatg tttttgttgg tattgatgcg
    23581 cccgacttca atattactct tgaaggtaac ctcaaaaagc agggtatcaa aaccattcat
    23641 tacgtcagtc catccgtctg ggcgtggcga cagaaacgcg ttttcaaaat aggcagagcc
    23701 accgatctgg tgctcgcatt tctgcctttc gaaaaagcgt tttatgacaa atacaatgta
    23761 ccgtgccgct ttatcggtca taccatggct gatgccatgc cgttagatcc agataaaaat
    23821 gccgcccgtg atgtgctggg gatccctcac gatgcccact gcctggcgtt gctaccgggg
    23881 agccgtggtg cagaagttga aatgcttagt gccgatttcc tgaaaacggc ccagcttttg
    23941 cgccagacat atccggatct cgaaatcgtg gtgccactgg tgaatgccaa acgccgcgag
    24001 cagtttgaac gcatcaaagc tgaagtcgcg ccagaccttt cagttcattt gctggatggg
    24061 atgggccgtg aggcgatggt cgccagcgat gcggcgctac tggcgtcggg tacggcagcc
    24121 ctggagtgta tgctggcgaa atgcccgatg gtggtgggat atcgcatgaa gccttttacc
    24181 ttctggttgg cgaagcggct ggtgaaaact gattatgtct cgctgccaaa tctgctggcg
    24241 ggcagagagt tagttaaaga attattgcag gaagagtgtg agccgcaaaa actggctgcg
    24301 gcgctgttac cgctgttggc gaacgggaaa accagccacg cgatgcacga taccttccgt
    24361 gaactgcatc agcagatccg ctgcaatgcc gatgagcagg cggcacaagc cgttctggag
    24421 ttagcacaat gatcgaattt gtttatccgc acacgcagct ggttgcgggt gtggatgaag
    24481 tcggacgcgg gccgttagtt ggcgcggtcg tcaccgctgc ggtgatcctt gacccggcgc
    24541 gcccgattgc cgggctgaat gattccaaaa agctgagcga aaaacgccgt ctggcgctct
    24601 atgaagagat caaagagaaa gcgttgagct ggagtctggg ccgcgcggaa ccccacgaaa
    24661 tcgacgagct gaacattctt catgcgacca tgctggcgat gcagcgtgcc gtcgctgggc
    24721 tgcatattgc gccggaatat gtgttgattg atggtaaccg ctgcccgaaa ttaccgatgc
    24781 ctgcgatggc tgtggtgaaa ggcgatagcc gcgtaccgga aatcagtgcc gcgtctatcc
    24841 tggcgaaagt gacgcgtgac gccgaaatgg cggcgctgga tattgttttc ccgcaatatg
    24901 gttttgccca acacaaaggg tacccaaccg cttttcatct ggaaaaactg gctgaacacg
    24961 gcgcgaccga acaccatcgg cgcagctttg ggcctgtcaa acgcgcactg ggacttgcgt
    25021 cctgattctt gtgtcgagat taagtaaacc ggaatctgaa gatgtctgaa ccacgtttcg
    25081 tacacctgcg ggtgcacagc gactactcga tgatcgatgg cctggccaaa accgcaccgt
    25141 tggtaaaaaa ggcggcggcg ttgggtatgc cagcactggc gatcaccgat ttcaccaacc
    25201 tttgtggtct ggtgaagttc tacggagcgg gacatggcgc agggattaag cctatcgtcg
    25261 gggcagattt taacgtccag tgcgacctgc tgggtgatga gttaacccac ctgacggtac
    25321 tggcggcgaa caataccggc tatcagaatc tgacgttgct gatctcaaaa gcgtatcagc
    25381 gcgggtacgg tgccgccggg ccgatcatcg atcgcgactg gcttatcgaa ttaaacgaag
    25441 ggttgatcct tctttccggc ggacgcatgg gcgacgtcgg acgcagtctt ttgcgtggta
    25501 acagcgcgct ggtagatgag tgtgtcgcgt tttatgaaga acacttcccg gatcgctatt
    25561 ttctcgagct gatccgcacc ggcaggccgg acgaagaaag ctatctgcac gcggcggtgg
    25621 aactggcgga agcgcgcggt ttgcccgtcg tggcgaccaa cgacgtgcgc tttatcgaca
    25681 gcagcgactt tgacgcacac gaaatccgcg tcgcgatcca cgacggcttt accctcgacg
    25741 atcctaaacg cccgcgtaac tattcgccgc agcaatatat gcgtagcgaa gaggagatgt
    25801 gcgagctgtt tgccgacatc cccgaagctc ttgccaacac cgttgagatc gccaaacgtt
    25861 gtaacgtaac cgtgcgtctt ggtgaatact tcctgccgca gttcccgacc ggggacatga
    25921 gcaccgaaga ttatctggtc aagcgtgcaa aagagggcct ggaagagcgt ctggcctttt
    25981 tattccctga cgaggaagaa cgtgttaagc gccgcccgga atatgacgaa cgtctggaga
    26041 ctgaacttca ggttatcaac cagatgggct tcccgggcta cttcctcatc gttatggaat
    26101 ttatccagtg gtcgaaagat aacggcgtac cggtagggcc aggccgtggc tccggtgcgg
    26161 gttcactggt ggcctacgcg ctgaaaatca ccgacctcga tccgctggaa tttgacctgc
    26221 tgttcgaacg tttccttaac ccggaacgtg tctccatgcc tgacttcgac gttgacttct
    26281 gtatggagaa acgcgatcag gttatcgagc acgtagcgga catgtacggt cgtgatgcgg
    26341 tatcgcagat catcaccttc ggtacaatgg cggcgaaagc ggtgatccgc gacgtaggcc
    26401 gcgtgctggg gcatccgtac ggctttgtcg atcgtatctc gaaactgatc ccgcccgatc
    26461 cggggatgac gctggcgaaa gcgtttgaag ccgagccgca gctgccggaa atctacgaag
    26521 cggatgaaga agttaaggcg ctgatcgaca tggcgcgcaa actggaaggg gtcacccgta
    26581 acgccggtaa gcacgccggt ggggtggtta tcgcgccgac caaaattacc gattttgcgc
    26641 cgctttactg cgatgaagag ggcaaacatc cggtcaccca gtttgataaa agcgacgttg
    26701 aatacgccgg actggtgaag ttcgacttcc ttggtttgcg tacgctcacc atcatcaact
    26761 gggcgctgga gatgatcaac aagcggcggg cgaagaatgg cgagccgccg ctggatatcg
    26821 ctgcgatccc gctggatgat aagaaaagct tcgacatgct gcaacgctcg gaaaccacgg
    26881 cggtattcca gcttgaatcg cgcggcatga aggacctgat caagcgtctg caacctgact
    26941 gcttcgaaga tatgatcgca ctggtggcac tgttccgccc cggcccgttg cagtcaggga
    27001 tggtggataa ctttatcgac cgtaaacatg gtcgcgaaga gatttcctat ccggacgtac
    27061 agtggcagca tgaaagcctg aaaccggtac tggagccaac ctacggcatc atcctgtatc
    27121 aggaacaggt catgcagatt gcgcaggtgc tttctggtta taccctcggt ggcgcggata
    27181 tgctgcgtcg tgcgatgggt aagaaaaagc cggaagagat ggctaagcag cgttctgtat
    27241 ttgctgaagg tgcagaaaag aacggaatca acgccaaact ggcgatgaaa atcttcgacc
    27301 tggtggagaa attcgcgggt tacggattta acaaatcgca ctctgcggcc tatgctttgg
    27361 tgtcatatca aacgttatgg ctgaaagcgc actatccggc ggagtttatg gcggcggtaa
    27421 tgaccgccga tatggacaac accgagaagg tggtgggcct ggtggatgag tgctggcgga
    27481 tggggctgaa aatcctgcca ccagatataa actccggtct ttaccatttc cacgtcaacg
    27541 acgacggcga aatcgtgtat ggtattggcg ccatcaaagg ggtaggtgaa ggtccgattg
    27601 aggccatcat cgaagcccgt aataaaggcg gctacttccg cgaactgttt gatctctgcg
    27661 cccgtaccga caccaaaaag ttaaaccggc gggtgctgga aaaactgatc atgtccgggg
    27721 cgtttgaccg tcttgggcca caccgcgcgg cgctgatgaa ctcgctgggt gatgcgttaa
    27781 aagcggcaga tcaacacgcg aaagcggaag ctatcggtca ggccgatatg ttcggcgtgc
    27841 tggccgaaga gccggaacaa attgaacaat cctacgccag ctgccaaccg tggccggagc
    27901 aggtggtgtt agatggggaa cgtgaaacgt taggtctgta ccttacggga caccctatca
    27961 accagtattt aaaagagatt gagcgttatg tcggaggcgt aaggctgaaa gacatgcacc
    28021 cgacagaacg tggtaaagtc atcacggctg cggggctcgt tgttgccgcg cgggttatgg
    28081 tcaccaagcg cggcaatcgt atcggtatct gcacgctgga tgaccgttcc gggcggctgg
    28141 aagtgatgtt gtttactgac gccctggata aataccagca attgctggaa aaagaccgca
    28201 tacttatcgt cagcggacag gtcagctttg atgacttcag cggtgggctt aaaatgaccg
    28261 ctcgcgaagt gatggatatt gacgaagccc gggaaaaata tgctcgcggg cttgctatct
    28321 cgctgacgga caggcaaatt gatgaccagc ttttaaaccg actccgtcag tctctggaag
    28381 cccaccgctc tgggacaatt ccagtacatc tctactatca gagggcggat gcacgcgcgc
    28441 ggttgcgttt tggcgcgacg tggcgtgtct ctccgagcga tcgtttatta aacgatctcc
    28501 gtggcctcat tggttcggag caggtggaac tggagtttga ctaatacagg aatactatga
    28561 gtctgaattt ccttgatttt gaacagccga ttgcagagct ggaagcgaaa atcgattctc
    28621 tgactgcggt tagccgtcag gatgagaaac tggatattaa catcgatgaa gaagtgcatc
    28681 gtctgcgtga aaaaagcgta gaactgacac gtaaaatctt cgccgatctc ggtgcatggc
    28741 agattgcgca actggcacgc catccacagc gtccttatac cctggattac gttcgcctgg
    28801 catttgatga atttgacgaa ctggctggcg accgcgcgta tgcagacgat aaagctatcg
    28861 tcggtggtat cgcccgtctt gacggtcgtc cggtgatgat cattggtcat caaaaaggtc
    28921 gtgaaaccaa agaaaaaatt cgccgtaact ttggtatgcc agcgccagaa ggttaccgca
    28981 aagcactgcg tctgatgcaa atggctgaac gctttaagat gcctatcatc acctttatcg
    29041 acaccccggg ggcttatcct ggcgtgggcg cagaagagcg tggtcagtct gaagccattg
    29101 cacgcaacct gcgtgaaatg tctcgcctcg gcgtaccggt agtttgtacg gttatcggtg
    29161 aaggtggttc tggcggtgcg ctggcgattg gcgtgggcga taaagtgaat atgctgcaat
    29221 acagcaccta ttccgttatc tcgccggaag gttgtgcgtc cattctgtgg aagagcgccg
    29281 acaaagcgcc gctggcggct gaagcgatgg gtatcatcgc tccgcgtctg aaagagctga
    29341 aactgatcga ctccatcatc ccggaaccgc tgggcggcgc tcaccgtaac ccggaagcga
    29401 tggcggtatc gttgaaagcg caactgctgg cggatctggc cgatctcgat gtgttaagca
    29461 ctgaagattt aaaaaatcgt cgttatcagc gcctgatgag ctacggttac gcgtaattcg
    29521 caaaagttct gaaaaagggt cacttcgg
//
LOCUS       DADMKF010000038        29058 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.039, whole genome shotgun sequence.
ACCESSION   DADMKF010000038 DADMKF010000000
VERSION     DADMKF010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29058
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.039"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(86..1369)
                     /gene="gltA"
                     /locus_tag="JNC24_001534"
     CDS             complement(86..1369)
                     /gene="gltA"
                     /locus_tag="JNC24_001534"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HAY9158310.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSDGMKIARPRQLYTGYEKRDFKSDIKR"
     gene            complement(1575..1682)
                     /locus_tag="JNC24_001535"
     CDS             complement(1575..1682)
                     /locus_tag="JNC24_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9158311.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            2063..2467
                     /gene="sdhC"
                     /locus_tag="JNC24_001536"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             2063..2467
                     /gene="sdhC"
                     /locus_tag="JNC24_001536"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HAY9158312.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            2461..2808
                     /gene="sdhD"
                     /locus_tag="JNC24_001537"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             2461..2808
                     /gene="sdhD"
                     /locus_tag="JNC24_001537"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HAY9158313.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            2808..4574
                     /gene="sdhA"
                     /locus_tag="JNC24_001538"
     CDS             2808..4574
                     /gene="sdhA"
                     /locus_tag="JNC24_001538"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HAY9158314.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASESDVEASLDRLHRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            4590..5306
                     /locus_tag="JNC24_001539"
     CDS             4590..5306
                     /locus_tag="JNC24_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HAY9158315.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYPLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            5607..8408
                     /gene="sucA"
                     /locus_tag="JNC24_001540"
     CDS             5607..8408
                     /gene="sucA"
                     /locus_tag="JNC24_001540"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HAY9158316.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKENMKLGELLEAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRTASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            8423..9640
                     /gene="odhB"
                     /locus_tag="JNC24_001541"
     CDS             8423..9640
                     /gene="odhB"
                     /locus_tag="JNC24_001541"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HAY9158317.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEDPTRLLLDV"
     gene            9734..10900
                     /gene="sucC"
                     /locus_tag="JNC24_001542"
     CDS             9734..10900
                     /gene="sucC"
                     /locus_tag="JNC24_001542"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HAY9158318.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            10900..11769
                     /gene="sucD"
                     /locus_tag="JNC24_001543"
     CDS             10900..11769
                     /gene="sucD"
                     /locus_tag="JNC24_001543"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HAY9158319.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGATASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(11873..12595)
                     /gene="mngR"
                     /locus_tag="JNC24_001544"
     CDS             complement(11873..12595)
                     /gene="mngR"
                     /locus_tag="JNC24_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HAY9158320.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEILIF
                     EVIPADDFLQQQLQITAQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            12704..14680
                     /gene="mngA"
                     /locus_tag="JNC24_001545"
     CDS             12704..14680
                     /gene="mngA"
                     /locus_tag="JNC24_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HAY9158321.1"
                     /translation="MVLFYRAHWRDYKNDQVRIMMNLTTLTHRDALCLNARFTSREEA
                     IHALTQRLAALGKISSTEQFLEEVYRRESLGPTALGEGLAVPHGKTAAVKEAAFAVAT
                     LSEPLQWEGVDGPEAVDLVVLLAIPPNEAGTTHMQLLTALTTRLADDEIRARIQSATT
                     PDELLSALDDKGGTQPSASFSNAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNVY
                     VEKQGANGIEGRLTADQLNSATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEAL
                     IQQALTLKRSDETRTVQQDTQPVKSVKTELKQALLSGISFAVPLIVAGGTVLAVAVLL
                     SQIFGLQDLFNEENSWLWMYRKLGGGLLGILMVPVLAAYTTYSLADKPALAPGFAAGL
                     AANMIGSGFLGAVVGGLIAGYLMRWVKNHLRLSSKFNGFLTFYLYPVLGTLGAGSLML
                     FVVGEPVAWINNSLTAWLNGLSGSNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMA
                     NGVYGPYAIFASVKMVSAFTVTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPM
                     AIEDPLRVIGSFVLGSMVTGAIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWF
                     GAALVGAAISTAILLIWRRHAVKHGNYLTDGVMP"
     gene            14698..17254
                     /locus_tag="JNC24_001546"
                     /pseudo
     CDS             14698..17254
                     /locus_tag="JNC24_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415260.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
     gene            17279..17506
                     /locus_tag="JNC24_001547"
     CDS             17279..17506
                     /locus_tag="JNC24_001547"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9158322.1"
                     /translation="MRGQIVDKGRFVHAGYGMNALFGLQKQANSIYCRDDVDTGKRSA
                     SGNFAFIFTLKPRICGFIFNKIITLGELIII"
     gene            18102..19670
                     /gene="cydA"
                     /locus_tag="JNC24_001548"
     CDS             18102..19670
                     /gene="cydA"
                     /locus_tag="JNC24_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HAY9158323.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
     gene            19686..20825
                     /gene="cydB"
                     /locus_tag="JNC24_001549"
     CDS             19686..20825
                     /gene="cydB"
                     /locus_tag="JNC24_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HAY9158324.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            20840..20953
                     /gene="cydX"
                     /locus_tag="JNC24_001550"
     CDS             20840..20953
                     /gene="cydX"
                     /locus_tag="JNC24_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HAY9158325.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            20953..21246
                     /gene="ybgE"
                     /locus_tag="JNC24_001551"
     CDS             20953..21246
                     /gene="ybgE"
                     /locus_tag="JNC24_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HAY9158326.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            21396..21800
                     /gene="ybgC"
                     /locus_tag="JNC24_001552"
     CDS             21396..21800
                     /gene="ybgC"
                     /locus_tag="JNC24_001552"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HAY9158327.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPCALPKSIVAEFKQ"
     gene            21797..22489
                     /gene="tolQ"
                     /locus_tag="JNC24_001553"
     CDS             21797..22489
                     /gene="tolQ"
                     /locus_tag="JNC24_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HAY9158328.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            22493..22921
                     /gene="tolR"
                     /locus_tag="JNC24_001554"
     CDS             22493..22921
                     /gene="tolR"
                     /locus_tag="JNC24_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HAY9158329.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            22986..24218
                     /gene="tolA"
                     /locus_tag="JNC24_001555"
     CDS             22986..24218
                     /gene="tolA"
                     /locus_tag="JNC24_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HAY9158330.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALLWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKIKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEEAAAKAAADAKAKAEADAKAAEEAA
                     KKAAADAKKKAEAEAAKAAAEAQKKAEAAAAALKKKAEAAEAAAAEARKKAAAEKAAA
                     DKKAAEKAAADKAAADKKAAAEKAAADKKAAAAKAAAEKAAADKKAAAAKAAAEKAAA
                     AKAAAEADDIFGELSSGKNAPKTGGGAKGNNASSAGSGNTKNNGASGADINNYAGQIK
                     SAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAALAAAKLAKIPKP
                     PSQAVYEVFKNAPLDFKP"
     gene            24351..25643
                     /gene="tolB"
                     /locus_tag="JNC24_001556"
     CDS             24351..25643
                     /gene="tolB"
                     /locus_tag="JNC24_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolB"
                     /protein_id="HAY9158331.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            25678..26199
                     /gene="pal"
                     /locus_tag="JNC24_001557"
     CDS             25678..26199
                     /gene="pal"
                     /locus_tag="JNC24_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HAY9158332.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            26209..27000
                     /gene="cpoB"
                     /locus_tag="JNC24_001558"
     CDS             26209..27000
                     /gene="cpoB"
                     /locus_tag="JNC24_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HAY9158333.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGAAASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            27165..27240
                     /locus_tag="JNC24_001559"
     tRNA            27165..27240
                     /locus_tag="JNC24_001559"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:27198..27200,aa:Lys,seq:ttt)
     gene            27390..27465
                     /locus_tag="JNC24_001560"
     tRNA            27390..27465
                     /locus_tag="JNC24_001560"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:27423..27425,aa:Val,seq:tac)
     gene            27469..27544
                     /locus_tag="JNC24_001561"
     tRNA            27469..27544
                     /locus_tag="JNC24_001561"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:27502..27504,aa:Lys,seq:ttt)
     gene            27593..27668
                     /locus_tag="JNC24_001562"
     tRNA            27593..27668
                     /locus_tag="JNC24_001562"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:27626..27628,aa:Lys,seq:ttt)
     gene            27702..27777
                     /locus_tag="JNC24_001563"
     tRNA            27702..27777
                     /locus_tag="JNC24_001563"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:27735..27737,aa:Lys,seq:ttt)
     gene            27802..27933
                     /locus_tag="JNC24_001564"
     ncRNA           27802..27933
                     /ncRNA_class="other"
                     /locus_tag="JNC24_001564"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            28182..>29058
                     /gene="nadA"
                     /locus_tag="JNC24_001565"
     CDS             28182..>29058
                     /gene="nadA"
                     /locus_tag="JNC24_001565"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HAY9158334.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMLTLQAECSLDLGCPVEEFNAFCDAHPDHTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSFGEKIIWAPDKHLGRYVQKQTGADILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKTLPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCR"
BASE COUNT         7107 a         7099 c         7825 g         7027 t
ORIGIN      
        1 ttacaatttt ttgtgtttgg gcatataaaa attaacccgc catttgaacg gcgggttaaa
       61 atatttacaa cttagcaatc aaccattaac gcttgatatc gcttttaaag tcgcgttttt
      121 catatcctgt atacagctga cgcggacggg caatcttcat accgtcactg tgcatttcgc
      181 tccagtgggc gatccagcca acggtacgtg ccattgcgaa aatgacggtg aacatggaag
      241 acggaatacc catcgctttc aggatgatac cagagtagaa atcgacgttc gggtacagtt
      301 tcttctcgat aaagtacggg tcgttcagcg cgatgttttc cagctccata gccacttcca
      361 gcaggtcatc tttggtgccc agctctttca gaacttcatg gcaggtttca cgcattacgg
      421 tggcgcgcgg gtcgtaattt ttgtacacgc ggtgaccgaa gcccatcagg cggaaagaat
      481 catttttatc tttcgcacga cgaacaaatt ccggaatgtg tttaacggag ctaatttctt
      541 ccagcatttt cagcgccgct tcgttagcac caccgtgcgc aggtccccac agtgaagcaa
      601 tacctgctgc gatacaggca aacgggttcg cacccgaaga gccagcggta cgcacggtgg
      661 aggtagaggc gttctgttca tggtcagcgt gcaggatcag aatacggtcc atagcacgtt
      721 ccagaatcgg attaacttca tacggttcgc acggcgtgga gaacatcata ttcaggaagt
      781 taccggcata ggagagatcg ttgcgcggat aaacaaatgg ctgaccaatg gaatacttgt
      841 aacacatcgc ggccatggtc ggcattttcg acagcaggcg gaacgcggca atttcacggt
      901 gacgaggatt gttaacatcc agcgagtcgt gatagaacgc cgccagcgcg ccggtaatac
      961 cacacatgac tgccattgga tgcgagtcgc gacggaaagc atggaacaga cgggtaatct
     1021 gctcgtggat catggtatga cgggtcaccg tagttttaaa ttcgtcatac tgttcctgag
     1081 tcggtttttc accattcagc aggatgtaac aaacttccag gtagttagaa tcggtcgcca
     1141 gctgatcgat cgggaaaccg cggtgcagca aaataccttc atcaccatca ataaaagtaa
     1201 ttttagattc gcaggatgcg gttgaagtga agcctgggtc aaaggtgaac acaccttttg
     1261 aaccgagagt acggatatca ataacatctt gacccagcgt gcctttcagc acatccagtt
     1321 caacagctgt gtccccgttg agggtgagtt ttgcttttgt atcagccatt taaggtctcc
     1381 ttagcgcctt attgcgtaag actgccggaa cttaaatttg ccttcgcaca tcaacctggc
     1441 tttacccgtt tgttatttgg ctcgccgctc tgtgaaagag gggaaaacct gggtacagag
     1501 ctctgggcgc ttgcaggtaa aggatccatt gatgacgaat aaatggcgaa tcaagtactt
     1561 agcaatccga attattaaac ttgtctacca ctaataactg tcccgaatga attggtcaat
     1621 acttccacac tgttacataa gttaatctta ggtgaaatac cgacttcata acttttacgc
     1681 attatatgct tttcctggca atgtttgtaa caactttgtt gaatgattgt caaattagat
     1741 gattaaaaat taaataaatg ttgttatcgt gacctggatc actgttcagg ataaaacccg
     1801 acaaactata tgtaggttaa ttgtaatgat tttgtgaaca gcctatactg ccgccaggtc
     1861 tccggaacac cctgcaatcc cgagccaccc agcgttgtaa cgtgtcgttt tcgcatctgg
     1921 aagcagtgtt ttgcatgacg cgcagttata gaaaggacgc tgtctgaccc gcaagcagac
     1981 cggaggaagg aaatcccgac gtctccaggt aacagaaagt taacctctgt gcccgtagtc
     2041 cccagggaat aataagaaca gcatgtgggc gttattcatg ataagaaatg tgaaaaaaca
     2101 aagacctgtt aatctggacc tacagaccat ccggttcccc atcacggcga tagcgtccat
     2161 tctccatcgc gtttccggtg tgatcacctt tgttgcagtg ggcatcctgc tgtggcttct
     2221 gggtaccagc ctctcttccc ctgaaggttt cgagcaagct tccgcgatta tgggcagctt
     2281 cttcgtcaaa tttatcatgt ggggcatcct taccgctctg gcgtatcacg tcgtcgtagg
     2341 tattcgccac atgatgatgg attttggcta tctggaagaa acattcgaag cgggtaaacg
     2401 ctccgccaaa atctcctttg ttattactgt cgtgctttca cttctcgcag gagtcctcgt
     2461 atggtaagca acgcctccgc attaggacgc aatggcgtac atgatttcat cctcgttcgc
     2521 gctaccgcta tcgtcctgac gctctacatc atttatatgg tcggtttttt cgccaccagt
     2581 ggcgaactga catatgaagt ctggatcggt ttcttcgcct ctgcgttcac caaagtgttc
     2641 accctgctgg cgctgttttc tatcttgatt catgcctgga tcggcatgtg gcaggtgttg
     2701 accgactacg ttaaaccgct ggcattgcgc ctgatgctgc aactggtgat tgtcgttgca
     2761 ctggtggttt acgtgattta tggattcgtt gtggtgtggg gtgtgtgatg aaattgccag
     2821 tcagagaatt tgatgcagtt gtgattggtg ccggtggcgc aggtatgcgc gcggcgctgc
     2881 aaatttccca gagcggccag acctgtgcgc tgctctctaa agtcttcccg acccgttccc
     2941 ataccgtttc tgcgcaaggc ggcattaccg ttgcgctggg taatacccat gaagataact
     3001 gggaatggca tatgtacgac accgtaaaag ggtcggacta tatcggtgac caggacgcga
     3061 ttgaatatat gtgtaaaacc gggccggaag cgattctgga actcgaacac atgggcctgc
     3121 cgttctcgcg tcttgatgat ggtcgtatct atcaacgtcc gtttggcggt cagtcgaaaa
     3181 acttcggcgg cgagcaggcg gcacgcactg cggcggcggc tgaccgtacc ggtcacgcac
     3241 tgttgcacac gctttatcag cagaacctga aaaaccacac caccattttc tccgagtggt
     3301 atgcgctgga tctggtgaaa aaccaggatg gcgcggtggt gggttgtacc gcactgtgca
     3361 tcgaaaccgg tgaagtggtt tatttcaaag cccgcgctac cgtgctggcg actggcgggg
     3421 cagggcgtat ttatcagtcc accactaacg cccacattaa cactggcgac ggtgtcggca
     3481 tggctatccg cgcaggcgta ccggtgcagg atatggaaat gtggcaattc cacccgaccg
     3541 gcattgccgg tgcgggcgta ctggtcaccg aaggttgccg tggtgaaggc ggttatctgc
     3601 tgaacaaaca tggcgaacgc tttatggagc gttatgcgcc gaacgccaaa gacctggcgg
     3661 gccgtgatgt ggtggcgcgt tccatcatga ttgaaatccg tgaaggtcgc ggctgtgatg
     3721 gtccgtgggg gccacacgcg aaactgaaac tcgatcacct gggtaaagaa gttctcgaat
     3781 cccgtctgcc gggtatcctg gagctttccc gtacctttgc tcacgtcgat ccggtgaaag
     3841 agccgattcc ggttatccca acctgtcact acatgatggg cggtattccg accaaagtga
     3901 ccggtcaggc gctgacggtg aacgagaaag gcgaagatgt ggttgttccg gggctgtttg
     3961 ccgttggtga aatcgcttgt gtatcggtac acggcgctaa ccgtctgggc ggcaactcgc
     4021 tgctggacct ggtggtcttt ggtcgtgcgg caggtctgca tctgcaagag tctatcgccg
     4081 aacagggcgc attgcgcgat gccagcgagt ctgatgttga agcatctctg gatcgtctgc
     4141 accgctggaa caataatcgt aacggtgaag atccggtggc gattcgtaaa gcgctgcaag
     4201 aatgtatgca gcataacttc tcggtcttcc gtgaaggtga tgcgatggcg aaagggcttg
     4261 agcagttgaa agtgatccgc gagcgtctga aaaatgcccg tctggatgac acttccagcg
     4321 agttcaacac ccagcgcgtt gagtgcttgg aactggataa cctgatggaa acggcgtatg
     4381 caacggctgt ttctgccaac ttccgtaccg aaagccgtgg cgcacatagc cgcttcgact
     4441 tcccggatcg tgatgatgaa aactggctgt gccactccct gtatctgcca gagtcggaat
     4501 ccatgacgcg ccgaagcgtc aacatggaac cgaaactgcg cccggcattc ccgccgaaga
     4561 ttcgtactta ctaatgcgga gacaggaaaa tgagactcga gttttcaatt tatcgctata
     4621 acccggatgt tgatgatgct ccgcgtatgc aggattaccc cctggaagcg gaagaaggtc
     4681 gcgacatgat gctgctggat gcgcttatcc agctgaaaga gaaagatccc agcctgtcgt
     4741 tccgccgctc ctgccgtgaa ggtgtgtgcg gttccgacgg tctgaacatg aacggcaaga
     4801 atggtctggc ctgtattacc ccgatttcgg cactcaacca gccgggcaag aagattgtga
     4861 ttcgcccgct gccaggttta ccggtgatcc gcgatttggt ggtagacatg ggacaattct
     4921 atgcgcaata tgagaaaatt aagccttacc tgttgaataa tggacaaaat ccgccagctc
     4981 gcgagcattt acagatgcca gagcagcgcg aaaaactcga cgggctgtat gaatgtattc
     5041 tctgcgcatg ttgttcaacc tcttgtccgt ctttctggtg gaatcccgat aagtttatcg
     5101 gcccggcagg cttgttagcg gcatatcgtt tcctgatcga tagccgtgat accgagactg
     5161 acagccgcct cgacggtttg agcgatgcat tcagcgtatt ccgctgtcac agcatcatga
     5221 actgcgtcag tgtatgtccg aaggggctga acccgacgcg cgccatcggc catattaagt
     5281 cgatgttgtt gcaacgtaat gcgtaaaccg taggcctgat aagacgcgca agcgtcgcat
     5341 caggcaacca gtgccggatg cggcgtgaac gccttatccg gcctacaagt cattacccgt
     5401 aggcctgata agcgcagcgc atcaggcgta acaaagaaat gcaggaaatc tttaaaaact
     5461 gcccctgaca ctaagacagt ttttaaaggt tccttcgcga gccactacat agacaagagc
     5521 tcgcaagtga accccggcac gcacatcact gtgcgtggta gtatccacgg cgaagtaagc
     5581 ataaaaaaga tgcttaaggg atcacgatgc agaacagcgc tttgaaagcc tggttggact
     5641 cttcttacct ctctggcgca aaccagagct ggatagaaca gctctatgaa gacttcttaa
     5701 ccgatcctga ctcggttgac gctaactggc gttcgacgtt ccagcagtta cctggtacgg
     5761 gagtcaaacc ggatcaattc cactctcaaa cgcgtgaata tttccgccgc ctggcgaaag
     5821 acgcttcacg ttactcttca acgatctccg accctgacac caatgtgaag caggttaaag
     5881 tcctgcagct cattaacgca taccgcttcc gtggtcacca gcatgcgaat ctcgatccgc
     5941 tgggactgtg gcagcaagat aaagtggccg atctggatcc gtctttccac gatctgaccg
     6001 aagcagactt ccaggagacc ttcaacgtcg gttcatttgc cagcggcaaa gaaaacatga
     6061 aactcggcga gctgctggaa gccctcaagc aaacctactg tggcccgatt ggtgccgagt
     6121 atatgcacat taccagcacc gaagaaaaac gctggatcca acagcgtatc gagtctggtc
     6181 gcgcgacttt caatagcgaa gagaaaaaac gcttcttaag cgaactgacc gccgctgaag
     6241 gccttgaacg ttacctcggc gcaaaattcc ctggcgcaaa acgcttctcg ctggaaggcg
     6301 gtgacgcgtt aatcccgatg cttaaagaga tgatccgcca cgctggcaac agcggcaccc
     6361 gcgaagtggt tcttgggatg gcgcaccgtg gtcgtctgaa cgtgctggtg aacgtgctgg
     6421 gtaaaaaacc gcaagacttg ttcgacgagt ttgccggtaa acataaagaa cacctcggca
     6481 cgggtgacgt gaaataccac atgggcttct cgtctgactt ccagaccgat ggcggcctgg
     6541 tgcacctggc gctggcgttt aacccgtctc accttgagat tgtaagcccg gtagttatcg
     6601 gttctgttcg tgcccgtctg gacagacttg atgagccgag cagcaacaaa gtgctgccaa
     6661 tcaccatcca cggtgacgcc gcagtgaccg ggcagggcgt ggttcaggaa actctgaaca
     6721 tgtcgaaagc gcgtggttat gaagttggcg gtacggtacg tatcgttatc aacaaccagg
     6781 ttggtttcac cacctctaat ccactggatg ctcgttctac accgtactgt actgatatcg
     6841 gtaagatggt tcaggcaccg attttccacg ttaacgcgga tgatccggaa gccgttgcct
     6901 ttgtgacccg tctggcgctc gatttccgta acacctttaa acgtgatgtc ttcatcgacc
     6961 tggtgtgcta ccgccgtcac ggccacaacg aagccgacga gccgagcgca acccagccgc
     7021 tgatgtatca gaaaatcaaa aaacatccga caccgcgcaa aatctacgct gacaagctgg
     7081 agcaggaaaa agtggcgacg ctggaagatg ccaccgagat ggttaacctg taccgcgatg
     7141 cgctggatgc tggcgattgc gtagtggcag agtggcgtcc gatgaacatg cactctttca
     7201 cctggtcgcc gtacctcaac cacgaatggg acgaagagta cccgaacaaa gttgagatga
     7261 agcgcctgca ggagctggcg aaacgcatca gcacggtgcc ggaagcagtt gaaatgcagt
     7321 ctcgcgttgc caagatttat ggcgatcgcc aggcgatggc tgccggtgag aaactgttcg
     7381 actggggcgg tgcggaaaac ctcgcttacg ccacgctggt tgacgaaggc attccggttc
     7441 gcctgtcggg tgaagactcc ggtcgcggta ccttcttcca ccgccacgcg gtgatccaca
     7501 accagtctaa cggttccact tacacgccgt tgcaacacat ccataacggg cagggcgcgt
     7561 tccgcgtctg ggactccgta ctgtctgaag aagcagtgct ggcgtttgaa tacggttatg
     7621 ccaccgcaga accacgcact ctgaccatct gggaagcgca gttcggtgac ttcgccaacg
     7681 gtgcgcaggt ggttatcgac cagttcatct cttctggcga acagaaatgg ggccggatgt
     7741 gtggtctggt gatgttgctg ccgcacggtt acgaagggca ggggccggag cactcctccg
     7801 cgcgtctgga acgttatctg caactttgtg ctgagcaaaa catgcaggtt tgcgttccgt
     7861 ctaccccggc acaggtttac cacatgctgc gtcgtcaggc gctgcgcggg atgcgtcgtc
     7921 cgctggtagt gatgtcgccg aaatccctgc tgcgtcatcc gctggcggtt tccagcctcg
     7981 aagaactggc gaacggcacc ttcctgccag ccatcggtga aatcgacgag cttgatccga
     8041 agggcgtgaa gcgcgtagtg atgtgttctg gtaaggttta ttacgacctg ctggaacagc
     8101 gtcgtaagaa caatcaacac gatgtcgcca ttgtgcgtat cgagcaactc tacccgttcc
     8161 cgcataaagc gatgcaggaa gtgttgcagc agtttgctca cgtcaaggat tttgtctggt
     8221 gccaggaaga gccgctcaac cagggcgcat ggtactgcag ccagcatcat ttccgtgaag
     8281 tgattccgtt tggggcttct ctgcgttatg caggccgcac ggcctccgcc tctccggcgg
     8341 tagggtatat gtccgttcac cagaaacagc aacaagatct ggttaatgac gcgctgaacg
     8401 tcgaataaat aaaggataca caatgagtag cgtagatatt ctggtccctg acctgcctga
     8461 atccgtagcc gatgccaccg tcgcaacctg gcataaaaaa cccggcgacg cagtcgtacg
     8521 tgatgaagtg ctggtagaaa tcgaaactga caaagtggta ctggaagtac cggcatcagc
     8581 agacggcatt ctggatgcgg ttctggaaga tgaaggtaca acggtaacgt ctcgtcagat
     8641 ccttggtcgc ctgcgtgaag gcaacagcgc cggtaaagaa accagcgcca aatctgaaga
     8701 gaaagcgtcc actccggcgc aacgccagca ggcgtctctg gaagagcaaa acaacgatgc
     8761 gttaagcccg gcgatccgtc gcctgctggc tgaacacaat ctcgacgcca gcgccattaa
     8821 aggcaccggt gtgggtggtc gtctgactcg tgaagatgtg gaaaaacatc tggcgaaagc
     8881 cccggcgaaa gagtctgctc cggcagcggc tgctccggcg gcgcaaccgg ctctggctgc
     8941 acgtagtgaa aaacgtgtcc cgatgactcg cctgcgtaag cgtgtggcag agcgtctgct
     9001 ggaagcgaaa aactccaccg ccatgctgac cacgttcaac gaagtcaaca tgaagccgat
     9061 tatggatctg cgtaagcagt acggtgaagc gtttgaaaaa cgccacggca tccgtctggg
     9121 ctttatgtcc ttctacgtga aagcggtggt tgaagccctg aaacgttacc cggaagtgaa
     9181 tgcttctatc gacggtgatg acgtggttta ccacaactat ttcgacgtca gcatggcggt
     9241 ttccacgccg cgcggcctgg tgacgccggt actgcgtgat gtcgataccc tcggcatggc
     9301 agacatcgag aagaaaatca aagagctggc agttaaaggc cgtgacggca agctgacggt
     9361 tgaagatctg accggtggta acttcaccat caccaacggt ggtgtgttcg gttccctgat
     9421 gtctacgccg atcatcaacc cgccgcagag cgcaattctg ggtatgcacg ctatcaaaga
     9481 tcgtccgatg gcggtgaatg gtcaggttga gatcctgccg atgatgtacc tggcgctgtc
     9541 ctacgatcac cgtctgatcg atggtcgcga atccgtgggc ttcctggtaa cgatcaaaga
     9601 gttgctggaa gatccgacgc gtctgctgct ggacgtgtag tagtttaagc ttcacctgca
     9661 ctgtagaccg acgtggatag ggtgtcggtc tacggtttaa aagataacga ttactgaagg
     9721 atggacagaa cacatgaact tacatgaata tcaggcaaaa caactttttg cccgctatgg
     9781 cttaccagca ccggtgggtt atgcctgtac tactccgcgc gaagcagaag aagccgcttc
     9841 aaaaatcggt gccggcccgt gggtagtgaa atgtcaggtt cacgctggtg gccgcggtaa
     9901 agcgggcggt gtgaaagttg taaacagcaa agaagacatc cgtgcttttg cagaaaactg
     9961 gctcggcaag cgtctggtaa cgtatcaaac agatgccaat ggccaaccgg ttaaccagat
    10021 tctggttgaa gcagcgaccg atatcgctaa agagctgtat ctcggtgccg ttgtagaccg
    10081 tagttcccgt cgtgtggtct ttatggcctc caccgaaggc ggcgtggaaa tcgaaaaagt
    10141 ggcggaagaa actccgcacc tgatccacaa agttgcgctt gatccgctga ctggcccgat
    10201 gccgtatcag ggacgcgagc tggcgttcaa actgggtctg gaaggtaaac tggttcagca
    10261 gttcaccaaa atcttcatgg gcctggcgac cattttcctg gagcgcgacc tggcgttgat
    10321 cgaaatcaac ccgctggtca tcaccaaaca gggcgatctg atttgcctcg acggcaaact
    10381 gggcgctgac ggcaacgcac tgttccgcca gcctgatctg cgcgaaatgc gtgaccagtc
    10441 gcaggaagat ccgcgtgaag cacaggctgc acagtgggaa ctgaactacg ttgcgctgga
    10501 cggtaacatc ggttgtatgg ttaacggcgc aggtctggcg atgggtacga tggacatcgt
    10561 taaactgcac ggcggcgaac cggctaactt ccttgacgtt ggcggcggcg caaccaaaga
    10621 acgtgtaacc gaagcgttca aaatcatcct ctctgacgac aaagtgaaag ccgttctggt
    10681 taacatcttc ggcggtatcg ttcgttgcga cctgatcgct gacggtatca tcggcgcggt
    10741 agcagaagtg ggtgttaacg taccggtcgt ggtacgtctg gaaggtaaca acgccgaact
    10801 cggcgcgaag aaactggctg acagcggcct gaatattatt gcagcaaaag gtctgacgga
    10861 tgcagctcag caggttgttg ccgcagtgga ggggaaataa tgtccatttt aattgataaa
    10921 aacaccaagg ttatctgcca gggctttact ggtagccagg ggactttcca ctcagaacag
    10981 gccattgcat acggcactaa aatggttggc ggcgtaaccc caggtaaagg cggtactacc
    11041 cacctcggcc tgccggtgtt caacaccgtg cgtgaagctg ttgctgctac tggcgctacc
    11101 gcttctgtta tctacgtacc agcaccgttc tgcaaagact ccattctgga agctattgat
    11161 gcaggcatca aactgattat caccatcact gaaggcatcc cgacgctgga tatgctgact
    11221 gtgaaagtga aactggacga agccggcgtt cgtatgatcg gcccgaactg cccgggcgtt
    11281 atcactccgg gtgaatgcaa aatcggtatc cagccgggtc acattcacaa accgggtaaa
    11341 gtgggtatcg tttcccgttc cggtacactg acctatgaag cggttaaaca gaccacggat
    11401 tacggtttcg gtcagtcgac ctgtgttggt atcggcggtg acccgatccc aggctctaac
    11461 tttatcgaca tcctcgaaat gttcgaaaaa gatccgcaga ccgaagcgat cgtgatgatc
    11521 ggtgagatcg gcggtagcgc tgaagaagaa gcggcagcgt acatcaaaga gcacgttacc
    11581 aagccagttg taggttacat cgctggtgtg actgcgccga aaggcaaacg tatgggccac
    11641 gcgggtgcga tcattgctgg tgggaaaggg actgcggatg agaaattcgc tgctctggaa
    11701 gccgcaggcg tgaaaaccgt tcgcagcctg gcggatatcg gtgaagcact gaaaactgtt
    11761 ctgaaataaa tatctgtaat aagaaatagc cctcgccgct tccctctaca ggaatggcga
    11821 agggctgtcg gtttcgacat ggttggccat cgtatgatgg ccttttttgt gcttatcgcg
    11881 atgattttcg ctgcgctatc agggtaaatt tatagtcatc ggtattaaaa gcgttgcggc
    11941 tatattcaaa cacccgacca tcaactaaat atccacgcga tactttttca agaatcggct
    12001 ttgtctggct gatattaagc agacggctca tctcttcggt tggcatcaga ggaatgattt
    12061 cctgttcgct acgatcgata accatcttct tcacttcttc aataaagtga tatttcgaat
    12121 tttccatgac ctgccaggtg agatccggga acaacgcaag cggcatccag gtttcttcca
    12181 gcgccattgg cttttgcttg cgatagcgca cgcgcttcac atgccacaca cgatcctgcg
    12241 cggtgatttg tagctgttgc tgaagaaaat cgtcagccgg aatcacttca aatatcagga
    12301 tttcactgtg tgtatcgacg tgacggtccg acagtttttc atcaaaactg gttaactgaa
    12361 aaatatcgta attgacccgc tcttctttga cgtaagtccc gctgccctga atgctttcga
    12421 ggatctgctg ctcgactagc tggcgcaaag cctgacgcac cgtaacccgg ctgacgccaa
    12481 actctgtttg tagcgctgat tcagtgggta acgcatcgcc aggtttaagc tcgccacgcg
    12541 caatttgttc acgaatgcga tcggcaatct gccggtataa gggcttgtgt cccattttta
    12601 gtatctcatt aatatgaatt tagccattat gcccgataaa ttcatcctgt aaataataca
    12661 aatacaatac aaataatttc aatcaagtga aattgatcac atgatggtat tgttttatcg
    12721 ggcacactgg cgcgactata aaaacgatca agtgaggatc atgatgaatc tgacgactct
    12781 gacccaccgc gatgcgttgt gtctgaatgc gcgctttacc agccgtgaag aggccatcca
    12841 cgcgttgact caacgtcttg ctgctctggg gaaaatttcc agtactgagc aatttctgga
    12901 agaagtgtat cgccgtgaaa gccttggccc gaccgcctta ggtgaagggt tggctgtgcc
    12961 gcatggcaaa actgctgcgg taaaagaagc ggcgtttgcg gtcgccacac tcagcgagcc
    13021 gcttcagtgg gaaggcgttg atggcccgga agcagttgat ttagtggtgc tgctggcgat
    13081 tccccccaat gaagcgggta ctacgcatat gcaactgctg acagcgctga ccacgcgcct
    13141 tgcggatgat gagattcggg cgcgtataca gtcggcgacg acgcctgatg agttgctctc
    13201 ggcgctggat gacaagggag gcacgcaacc ttctgcctct ttttccaacg cgccaactat
    13261 cgtctgcgta acggcctgtc cggcgggtat tgctcacacc tatatggctg cggaatatct
    13321 ggaaaaagcc ggacgcaaac tcggcgtaaa tgtttacgtt gaaaaacaag gcgctaacgg
    13381 cattgaaggg cgtttaacgg cggatcaact caatagtgca accgcctgta tttttgcggc
    13441 tgaagtcgcc atcaaggaga gtgagcgttt taatggcatt cccgcgcttt cagtgcctgt
    13501 tgccgagccg attcgccatg cagaagcgtt gatccaacaa gcgcttaccc tcaagcgtag
    13561 cgatgagacg cgtaccgtac agcaagatac gcaaccggtg aaaagtgtca aaacggagct
    13621 gaaacaggca ctgttgagcg gaatctcttt tgccgtaccg ttgattgtcg cggggggcac
    13681 ggtgctggcg gtcgcggtat tactgtcgca aatcttcggg ctacaagatc tgtttaatga
    13741 agaaaactcc tggctgtgga tgtaccgcaa gctgggcggc gggctgctcg gaattttgat
    13801 ggtaccggtg ctcgcggcct ataccaccta ttctctggca gataaaccgg cgttagcgcc
    13861 aggctttgcg gctggacttg ccgccaacat gatcggctcc gggtttctcg gcgcggtcgt
    13921 tggcggattg atagccggtt acttgatgcg ctgggtgaaa aatcacttgc gtcttagcag
    13981 taaattcaat ggattcctga ctttttatct ctacccggtg ctcggtacgt tgggagcggg
    14041 cagtctgatg ctgtttgtgg tgggagaacc tgtcgcatgg atcaataact cgcttaccgc
    14101 ctggctgaac ggtctgtcag gaagtaacgc gctgttgctg ggtgccattc tcggttttat
    14161 gtgttccttt gaccttggag ggccagtgaa caaagccgct tatgcattct gcctgggcgc
    14221 aatggcgaac ggcgtttacg gcccgtatgc cattttcgcc tccgtcaaaa tggtttcggc
    14281 atttaccgta accgcttcca cgatgctcgc accgcgcctg tttaaagagt ttgaaattga
    14341 gaccgggaaa tccacctggc tgttagggct ggcaggtatt accgaagggg cgatcccgat
    14401 ggcgattgaa gatccgctgc gggttattgg ttcgtttgtg ctgggctcta tggtaacggg
    14461 cgctattgtc ggtgcgatga atatcggcct ttcgacaccc ggtgccggca ttttctcgct
    14521 ctttttactt catgataatg gcgcgggcgg tgttatggcg gcaattggct ggtttggtgc
    14581 ggcattggtg ggggctgcaa tctcgactgc aattctcctg atctggcggc gtcacgcggt
    14641 taagcatggc aactatctga ctgatggcgt aatgccataa acaaaaagga aacgacgatg
    14701 aaagcggtat ctcgcgttca catcaccccg catatgcatt gggatcggga gtggtatttc
    14761 accaccgaag agtcacgcat tctgctggtc aataatatgg aagagatcct gtgccgactg
    14821 gaacaggaca acgaatacaa atattacgta ctcgacgggc aaacggcgat cctcgaagat
    14881 tatttcgcgg tgaaaccgga aaacaaagac cgcgtgaaga aacaggtaga agccggtaag
    14941 ttgattatcg gcccctggta tacccagacc gataccacga ttgtttctgc ggaatccatc
    15001 gtccgtaatc tgatgtacgg aatgcgtgac tgcctcgcgt ttggcgagcc gatgaaaata
    15061 ggttatttac cggattcctt tggcatgtcc gggcaactgc cgcatattta caatggattt
    15121 ggcattaccc gcactatgtt ctggcgcgga tgttcggagc gccacggtac tgataaaacc
    15181 gagtttttgt ggcaaagcag tgacggtagc gaagtgacgg cgcaggtgct gccgctgggc
    15241 tacgccatcg gtaagtactt acctgccgac gaaaacggat tacgtaaacg cctcgacagt
    15301 tattttgacg tgctggaaaa agcgtctgta accaaagaga ttttgctgcc gaatggtcat
    15361 gaccagatgc cattgcagca aaatatcttc gaagtgatgg ataagctacg tgagatctac
    15421 cctcaacgta agtttgtgat gagccgcttt gaagaggtat ttgagaagat cgaagcgcag
    15481 cgtgaaagtc tggccaccct gaaaggtgaa tttatcgatg gcaaatatat gcgcgtacat
    15541 cgcaccatcg gttccacgcg catggatatc aaaattgccc acgcacgtat tgaaaataag
    15601 attgttaatc tgctggaacc gctggcaacg ctggcctgga cgttgggttt tgaataccac
    15661 cacggcttgc tggagaaaat gtggaaagag atcttaaaaa atcatgccca cgacagtatc
    15721 ggttgttgtt gcagtgacaa agttcatcgc gaaattgtcg cccgcttcga actggctgaa
    15781 gacatggcgg ataatctgct gcgtttctac atgcgcaaaa ttgccgataa catgccacag
    15841 agtgatgccg acaaactcgt gctgtttaac ctgatgccct ggccgcggga agaagttatt
    15901 aacaccactg tgcggctgcg cgccagccag tttaatctac aagacgatcg cggtcagcct
    15961 gtaccgtatt ttattcgcca tgcccgtgag atcgatcctg gcctaatcga tcggcaaatt
    16021 gttcattatg gcaattatga tccctttatg gagtttgata ttcagatcaa ccagattgtc
    16081 ccttctatgg gctatcgcac gctttatatt gaagcgaatc agcctggcaa cgtgattgcg
    16141 gcaaaaagtg acgctgaagg gattctggaa aatgctttct ggcagattgc gctcaatgag
    16201 gatggttctc tgcaactggt agataaagac agcggtgtgc gctatgaccg ggtattgcaa
    16261 attgaagaaa gctctgatga tggtgatgaa tatgactatt cacccgcaaa agaagagtgg
    16321 gtaattaccg cagcgaacgc gaaaccgcaa tgcgatatta ttcatgaagc ctggcagagt
    16381 agggggggtg ttggttgtca ctcttagtca taacagcagg cgtattgatg tggatatcaa
    16441 ccttgataac caggctgacg atcatcgcct tcgtgtcctg gtccctacac cttttaacac
    16501 tgacagtgtt ctggcagata cgcagtttgg ttcgctaacg cgccccgtga acgacagtgc
    16561 aatgaacaac tggcagcaag aaggctggaa agaagcgccg gttccggtat ggaatatgct
    16621 caactatgtt gccttacagg aagggcgtaa cggcatggct gtctttagcg aagggttacg
    16681 tgaatttgaa gtcatcggtg aagagaagaa aacctttgcc attacgttgc tgcgtggcgt
    16741 gggcttactg ggcaaagaag atctgctttt aaggcctggg cggccttcgg gaattaaaat
    16801 gccagtcccg gactcacaac tacgtggtct gctttcttgt cgcctaagtt tattgagtta
    16861 taccggtacg ccaaccgccg ctggtgtagc tcagcaggcg cgagcatggc tgactccagt
    16921 acagtgttac aacaaaatcc catgggatgc gatgaagctc aacaaagccg gattcaacgt
    16981 gccggaaagt tatagtttgt tgaaaatgcc cccagtggga tgcctgataa gcgcacttaa
    17041 gaaagctgaa gaccgacaag aagtgatttt acggctgttt aatccggctg aatcagcaac
    17101 ctgtgatgcg actgttgctt tcagtcgcga ggtgatttct tgctcagaaa cgatgatgga
    17161 tgaacacatt accaccgagg aaaatcaagg ttcaaatcta tcggggcctt ttttacccgg
    17221 ccagtcacgg acgttcagtt accggcttgc ctgaatagca atcaaaccga agccacatat
    17281 gcgcggccag attgttgaca aagggcgctt tgttcatgcc ggatacggca tgaacgcttt
    17341 attcggtcta caaaagcagg caaattcaat atattgcaga gatgatgtag acactggcaa
    17401 gcgtagcgca tcaggtaatt ttgcgtttat cttcactctc aagccacgta tatgtggctt
    17461 tatttttaac aaaataataa ccctgggtga gttaattata atataattac aagttaatta
    17521 aatgttaata tcggcggggt ggatttatgc ctttattagt aatcctgaaa ctctgcgtcg
    17581 tattagccag tgaccaaaaa aagaattaag gtcaaccgtg ctgtttttgc ttcgtctctt
    17641 tttatcttta aattgccaac cgaaactaat ttcagcctta taactcacac attttaaaca
    17701 taaatgtcac taaagttacc ttattgaaac atgattaaca taatttgtag gaattgatat
    17761 ttatcaatgt ataagtcttg gaaattggca tcaaaaagag ataaattgtt ctcgatcaaa
    17821 ttggctgaaa ggcggtaatt tagctataaa ttgatcaccg tcgaaaaatg caaatttgct
    17881 tcaacaaaaa cctgtttatt gtaaggattt tgcagcgtaa tatatacgtg ggatcaattt
    17941 gagtttttat taacatgttt gcaacctttc tttacgccgt ttttgtgtgc attcacatgg
    18001 tatgatgaaa gtgttcaaac aaatttctat tggggcatgc gtgtgaccct ttctaacggg
    18061 gttcactctc ggagtcttca tgcgatgagc aaggagtcat gatgttagat atagtcgaac
    18121 tgtcgcgctt acagtttgcc ttgaccgcga tgtaccactt cctttttgtg ccactgacgc
    18181 tcggtatggc gttcctgctg gccattatgg aaacggtcta cgtcctctcc ggcaaacaga
    18241 tttataaaga tatgaccaag ttctggggca agttgtttgg tatcaacttc gctctgggtg
    18301 tggctaccgg tctgaccatg gagttccagt tcgggactaa ctggtcttac tattcccact
    18361 atgtagggga tatcttcggt gcgccgctgg caatcgaagg tctgatggcc ttcttcctcg
    18421 aatccacctt tgtaggtctg ttcttcttcg gttgggatcg tctgggtaaa gttcagcata
    18481 tgtgtgtcac ctggctggtg gcgctcggtt caaacctgtc cgcactgtgg attctggttg
    18541 cgaacggctg gatgcaaaac ccaatcgcgt ccgatttcaa ctttgaaact atgcgtatgg
    18601 agatggtgag cttctccgag ctggtgctta acccggttgc tcaggtgaaa ttcgttcaca
    18661 ctgtagcgtc tggttatgtg actggcgcga tgttcatcct cggtatcagc gcatggtata
    18721 tgctgaaagg tcgtgacttc gccttcgcta aacgctcctt tgctatcgct gccagcttcg
    18781 gtatggctgc tgttctgtct gttattgttc tgggtgatga atccggctac gaaatgggcg
    18841 acgtgcagaa aaccaaactg gctgctattg aagccgagtg ggaaacgcaa cctgcgcctg
    18901 ctgcctttac tctgttcggc attcctgatc aggaagagga gacgaacaaa tttgcgattc
    18961 agatccctta cgcactgggc atcattgcaa cgcgttccgt ggataccccg gttatcggcc
    19021 tgaaagagct gatggtgcag catgaagaac gcattcgtaa cgggatgaag gcgtactctc
    19081 tgctcgaaca actgcgttct ggttctaccg accaggcggt tcgtgaccag ttcaatagca
    19141 tgaagaaaga cctcggttac ggtctgctgc tgaaacgcta tacgccaaac gtggctgatg
    19201 cgactgaagc gcagattcaa caggcaacca aagactccat cccgcgtgta gcgccgctgt
    19261 actttgcgtt ccgtatcatg gtggcgtgtg gcttcctgct tctggcaatc atcgcgctct
    19321 ctttctggag tgtcatccgc aaccgcattg gcgagaaaaa atggcttctg cgcgccgcgc
    19381 tgtacggtat tccgctgccg tggattgctg tagaagcggg ctggttcgtg gctgaatatg
    19441 gccgccaacc gtgggctatc ggtgaagtgc tgccgacagc tgtggcgaac tcgtcactga
    19501 ccgcaggcga tctcatcttc tcaatggtgc tgatttgcgg cctgtatacc ctgttcctgg
    19561 tggcagaatt gttcttaatg ttcaagtttg cacgcctcgg cccaagcagc ctgaaaaccg
    19621 gtcgctatca ctttgagcag tcttccacga ctactcagcc ggcacgctaa gacaggagtc
    19681 gtcaaatgat cgattatgaa gtattgcgtt ttatctggtg gctgctggtt ggcgttctgc
    19741 tgattggttt tgcagtcact gacggtttcg acatgggggt gggcatgctc acccgtttcc
    19801 tcggtcgtaa cgacaccgag cgtcgaatta tgattaactc cattgcacca cactgggacg
    19861 gtaaccaggt ttggctgatc accgcgggcg gcgcactctt tgctgcctgg ccgatggtct
    19921 atgccgctgc gttctccggc ttctatgtgg cgatgatcct cgtgctggcg tctttgttct
    19981 tccgtccggt cggttttgac taccgctcca agattgaaga aacccgctgg cgtaacatgt
    20041 gggactgggg catcttcatt ggtagtttcg ttccgccgct ggtaattggt gtagcgttcg
    20101 gtaacctgtt gcagggcgta ccgttcaacg ttgatgaata tctgcgtctg tactacaccg
    20161 gtaacttctt ccagttgctt aacccgttcg gcctgctggc aggcgtggtg agcgtaggga
    20221 tgatcattac tcagggcgca acctatctgc aaatgcgtac cgtgggcgaa ctgcacctgc
    20281 gtacccgtgc aacggctcag gtggctgcgc tggtgacact ggtctgtttc gcactggctg
    20341 gcgtatgggt gatgtacggt atcgatggtt atgtcgtgaa atcgacaatg gaccattacg
    20401 cagcctctaa cccactgaat aaagaagtgg ttcgtgaagc tggcgcatgg ctggttaact
    20461 tcaacaacac gccaattctg tgggctattc cggcactggg tgtggttctt ccgctgctga
    20521 ccatcctgac tgcacgtatg gataaagccg cgtgggcgtt tgtgttctcc tccctgacgc
    20581 tggcctgcat catcctgaca gccggtatcg caatgttccc gtttgtgatg ccgtccagca
    20641 ccatgatgaa cgcaagtctg acaatgtggg atgcaacttc cagccagctg acgcttaacg
    20701 tcatgacctg ggttgcggtg gttctggtac cgatcattct gctctacacc gcttggtgtt
    20761 actggaaaat gttcggtcgt atcaccaaag aagatattga acgtaacacc cactctctgt
    20821 actaagtaag gagctaaaaa tgtggtattt cgcatggatt ctgggaacgc ttcttgcctg
    20881 ttcgtttggg gtaatcaccg cgctggcgct tgaacacgtc gaatcaggca aagccggtca
    20941 agaagacatc tgatgagtaa gattatcgcg accttgtatg cggtaatgga caagcgcccc
    21001 ctgcgggcgc tttccttcgt gatggcgctt ctgttagcag gatgtatgtt ttgggaccca
    21061 tctcgttttg ccgcgaagac cagtgaactg gaaatctggc atggtttatt gctgatgtgg
    21121 gccgtctgtg ctggtgtgat tcacggcgtg ggctttcgtc cgcagaaggt tctttggcaa
    21181 gggatttttt gcccattgct tgccgatatt gttctcattg tcgggctgat tttcttcttc
    21241 ttttaaatca gaattttctt taaaaaatta tgggccgctc caggcccata aatttttacg
    21301 ctcccttaac ttgccctcat tcccaaacct caatcgcgcg cgtatagtag cagcgtttaa
    21361 aagctctaac ttttgttgca ttaccgggat gtaaagtgaa tacaacgctg tttcgatggc
    21421 cggttcgcgt ctactatgaa gataccgatg ccggtggtgt ggtgtaccac gccagttacg
    21481 tcgcttttta tgaaagagca cgcacagaga tgctgcgtca tcatcacttc agtcagcagg
    21541 cgctgatggc tgaacgcgtt gcctttgtgg tacgtaaaat gacggtggaa tattacgcac
    21601 ctgcgcggct cgacgatatg ctcgaaatac agactgaaat aacatcaatg cgtggcacct
    21661 ctttggtttt cacgcaacgt attgtcaacg ccgagaatac tttgctgaat gaagcagagg
    21721 ttctggttgt ttgcgttgac ccactcaaaa tgaagccttg tgcgcttccc aagtctattg
    21781 tcgcggagtt taagcagtga ctgacatgaa tatccttgat ttgttcctga aggctagcct
    21841 tctggttaaa cttatcatgt tgattttgat tggtttttca atcgcatcct gggccattat
    21901 tatccagcgg acccgtattc ttaacgcagc ggcgcgcgaa gccgaagcgt ttgaagataa
    21961 attctggtct ggaatcgaac tctctcgcct ctatcaagag agtcagggga aacgggataa
    22021 tctgactggt tcggaacaaa tattttacag cgggttcaaa gagtttgtgc gcctgcatcg
    22081 tgccaatagc catgcgccgg aagccgtagt ggaaggggcg tcgcgtgcta tgcgtatctc
    22141 catgaaccgt gaacttgaaa atctggaaac gcacattccg ttcctcggta cggttggctc
    22201 catcagcccg tatattggtc tgtttggtac ggtctggggg atcatgcacg cctttatcgc
    22261 cctcggggcg gtaaaacaag caacactgca aatggttgcg cccggtatcg cagaagcgtt
    22321 gattgcgact gcaattggtc tgttcgccgc aattccggcg gtaatggctt ataaccgcct
    22381 caaccagcgc gtaaacaaac tggaactgaa ttacgacaac tttatggaag agtttaccgc
    22441 gattctgcac cgccaggcgt ttaccgttag cgagagcaac aaggggtaag ccatggccag
    22501 agcgcgtgga cgaggtcgtc gcgatctcaa gtccgaaatc aacattgtac cgttgctgga
    22561 cgtactgctg gtgctgttgc tgatctttat ggcgacagcg cccatcatca cccagagcgt
    22621 ggaggtcgat ctgccagacg ctactgaatc acaggcggtg agcagtaacg ataatccgcc
    22681 agtgattgtt gaagtgtctg gtattggtca gtacaccgtg gtggttgaga aagatcgcct
    22741 ggagcgttta ccaccagagc aggtggtggc ggaagtgtcc agccgtttca aggccaaccc
    22801 gaaaacggtc tttctgatcg gtggcgcaaa agatgtgcct tacgatgaaa taattaaagc
    22861 actgaacttg ttacatagtg cgggtgtgaa atcggttggt ttaatgacgc agcctatcta
    22921 aacatctgcg tttcccttgc ttgaaagaga gcgggtaaca ggcgaacagt ttttggaaac
    22981 cgagagtgtc aaaggcaacc gaacaaaacg acaagctcaa gcgggcgata attatttcag
    23041 cagtgctgca tgtcatctta tttgcggcgc tgctctggag ttcgttcgat gagaatatag
    23101 aagcttcagc cggaggcggc ggtggttcgt ccatcgacgc tgtcatggtt gattcaggtg
    23161 cggtagttga gcagtacaaa cgcatgcaaa gccaggaatc aagcgcgaag cgttctgatg
    23221 agcagcgcaa gataaaggaa cagcaggctg ctgaagaact gcgtgagaaa caagcggctg
    23281 aacaggaacg cctgaagcaa cttgagaaag agcggttagc tgctcaggaa cagaaaaagc
    23341 aggctgaaga agccgcaaaa caggccgagt taaagcagaa gcaagcggaa gaggcggcag
    23401 cgaaagcggc ggcagatgct aaagcgaagg ccgaagcgga tgctaaagct gcggaagaag
    23461 cagcgaagaa agcggcggca gacgcgaaga aaaaagcaga agcagaagcc gccaaagccg
    23521 cagccgaagc gcagaaaaaa gccgaggcag ccgctgcggc actgaagaag aaagcggaag
    23581 cggcagaagc tgctgcagct gaagcaagaa agaaagcggc agcagagaaa gctgcagccg
    23641 acaaaaaagc agcagaaaaa gcggctgctg ataaggcagc agctgataag aaagcagcgg
    23701 cagaaaaagc cgccgcagac aaaaaagcgg cagcggcaaa agcagcggca gaaaaagccg
    23761 ccgcagacaa aaaagcggca gcggcaaaag cagcagctga aaaagccgct gcagcaaaag
    23821 ctgccgcaga ggcagatgat attttcggtg agctaagctc tggtaagaat gcaccgaaaa
    23881 cggggggagg ggcgaaaggg aacaatgctt cgtctgccgg gagtggtaat actaaaaaca
    23941 atggcgcatc aggggccgat atcaataact atgccgggca gattaaatct gctatcgaaa
    24001 gtaagttcta tgacgcatcg tcctatgcag gcaaaacctg tacgctgcgc ataaaactgg
    24061 cacccgatgg tatgttactg gatatcaaac ctgaaggtgg cgatcccgca ctttgtcagg
    24121 ctgcgttggc agcagctaaa cttgcgaaga tcccgaaacc accaagccag gcagtatatg
    24181 aagtgttcaa aaacgcgcca ttggacttca aaccgtaatc gcgatgttga ctgttccgac
    24241 ggtcaacatc aggcaccggt tgccacgggg ttctggtagt tttgtgtatt ttagtttgtt
    24301 aacattctgc taaattatcg tgggccatcg gtccagataa gggagatatg atgaagcagg
    24361 cattacgagt agcatttggt tttctcatac tgtgggcatc agttctgcat gctgaagtcc
    24421 gcattgtgat cgacagcggt gtagattccg gtcgtcctat tggtgttgtt cctttccagt
    24481 gggcgggccc tggtgcggca cctgaagata ttggcggcat cgttgctgcc gacttgcgta
    24541 acagcggtaa atttaatccg ttagatcgcg ctcgtctgcc acagcagccg ggtagtgcgc
    24601 aggaagtaca accagctgca tggtccgcgc tgggtattga cgctgtagtt gtcggtcagg
    24661 tcactccgaa tccggatggt tcttacaatg ttgcttatca acttgttgac actggcggcg
    24721 caccgggtac tgtacttgct cagaactcgt acaaagtgaa caagcagtgg ctgcgctatg
    24781 ctggtcatac cgccagtgat gaagtgtttg aaaaactgac cggcattaaa ggtgcgttcc
    24841 gtacccgtat tgcctacgtt gttcagacca acggcggtca gttcccgtat gaactgcgcg
    24901 tatctgacta tgacggttac aaccagtttg tcgttcaccg ttcaccacag ccgctgatgt
    24961 ctccggcgtg gtcaccagac ggttctaaac tggcttatgt gaccttcgaa agcggtcgtt
    25021 ccgcgctagt tatccaaacg ctggcaaacg gcgctgtacg tcaggtggct tcattcccgc
    25081 gtcacaacgg tgcgcctgca ttctcgccag acggcagcaa actggcattc gccttgtcga
    25141 aaaccggtag cctgaacctg tacgtaatgg atttggcttc tggtcagatc cgccaggtga
    25201 ctgatggtcg cagtaacaat accgaaccga cctggttccc ggacagccag aacctggcat
    25261 ttacttctga ccaggccggt cgtccacagg tttataaagt gaatatcaac ggcggtgcgc
    25321 cacaacgtat tacctgggaa ggttcgcaga accaggatgc ggatgtcagc agcgacggta
    25381 aatttatggt aatggtcagc tccaacggtg ggcagcagca cattgccaaa caagatctgg
    25441 caacgggagg cgtacaagtt ctgtcgtcca cgttcctgga tgaaacgcca agtctggcac
    25501 ctaacggcac tatggtaatc tacagctctt ctcaggggat gggatccgtg ctgaatttgg
    25561 tttctacaga tgggcgtttc aaagcgcgtc ttccggcaac tgatggacag gtcaaattcc
    25621 ctgcctggtc gccgtatctg tgataataat taattgaata gtaaaggaat cattgaaatg
    25681 caactgaaca aagtgctgaa agggctgatg attgctctgc ctgttatggc aattgcggca
    25741 tgttcttcca acaagaacgc cagcaatgac ggcagcgaag gcatgctggg tgccggcact
    25801 ggtatggatg cgaacggcgg caacggcaac atgtcttccg aagagcaggc tcgtctgcaa
    25861 atgcaacagc tgcagcagaa caacatcgtt tacttcgatc tggacaagta cgatatccgt
    25921 tctgacttcg ctcaaatgct ggatgcacat gcaaacttcc tgcgtagcaa cccgtcttac
    25981 aaagtcaccg tagaaggtca cgcggacgaa cgtggtactc cggaatacaa catctccctg
    26041 ggtgaacgtc gtgcgaacgc cgttaagatg tacctgcagg gtaaaggcgt ttctgcagac
    26101 cagatctcca tcgtttctta cggtaaagaa aaacctgcag tactgggtca tgacgaagcg
    26161 gcatactcca aaaaccgtcg tgcggtactg gtttactaag agaattgcat gagcagtaac
    26221 ttcagacatc aactattgag tctgtcgtta ctggttggta tagcggcccc ctgggccgct
    26281 tttgctcagg caccaatcag tagtgtcggc tcaggctcgg tcgaagaccg cgtcactcaa
    26341 cttgagcgta tttctaatgc tcacagccag cttttaaccc aactccagca acaactttct
    26401 gataatcaat ccgatattga ttccctgcgt ggtcagattc aggaaaatca gtatcaactg
    26461 aatcaggtcg tggagcggca aaagcagatc ctgttgcaga tcgacagcct cagcagcggt
    26521 ggtgcagcgg cgcaatcaac cagcagcgat caaagcggtg cggcggcatc aacgacgccg
    26581 acagctgatg ctggtactgc gaatgctggc gcgccggtga aaagcggcga tgcaaacacg
    26641 gattacaatg cagctattgc gctggtgcag gataaatccc gccaggatga cgcaatggtg
    26701 gcatttcaga atttcatcaa aaattaccct gattcaactt acctgccaaa cgccaattat
    26761 tggctgggtc agttaaacta caacaagggt aaaaaagatg atgcggcgta ctattttgct
    26821 tcggtagtga aaaactatcc gaagtcacca aaggctgctg atgcgatgtt taaagtcggc
    26881 gtcatcatgc aggacaaagg tgacaccgca aaagcgaaag ccgtatacca gcaggttatc
    26941 agtaaatacc ctggtactga tggcgctaaa caggcacaaa aacgtctgaa cgcgatgtaa
    27001 tgcataacac acgaccagaa gtcgcattat ttctggtcgt gtcgtgcgaa tcataagcag
    27061 ttgagtgatc tgcatcgaaa tttttgttgc gctcaagtct gaaatcagta atatatgccg
    27121 ccgttgccac gggatatcaa acaaaccgaa agcaacgaaa aagtgggtcg ttagctcagt
    27181 tggtagagca gttgactttt aatcaattgg tcgcaggttc gaatcctgca cgacccacca
    27241 gttttagcat cgaagacaga tgttaagcgt gtaggataac gttgcgtcag caacggcccg
    27301 tagggcgagc gaagcgagtc atcctgcacg acccaccact aattacggtg ggttcggtgg
    27361 aagtagtttg tagtatccag cgcagtatcg ggtgattagc tcagctggga gagcacctcc
    27421 cttacaagga gggggtcggc ggttcgatcc cgtcatcacc caccactcgg gtcgttagct
    27481 cagttggtag agcagttgac ttttaatcaa ttggtcgcag gttcgaatcc tgcacgaccc
    27541 accaatctta aagattggcc ccgagtagaa atctttcagg taacacccgt atgggtcgtt
    27601 agctcagttg gtagagcagt tgacttttaa tcaattggtc gcaggttcga atcctgcacg
    27661 acccaccatc ctgaatgatt aaagcagcat aatcccgcaa ggggtcgtta gctcagttgg
    27721 tagagcagtt gacttttaat caattggtcg caggttcgaa tcctgcacga cccaccaatg
    27781 taaaaaagcg ccctaaaggc gcttttttgc tatctgagat attcaaagat tcgaacctgc
    27841 agcaggttcg agttgagcgc agcgaaacaa cggagccgct cgcggcgacg gcccgaaggg
    27901 cgagcgaagc gagtcatcct gcacgaccca ccaatgtaaa aaagcgccct aaaggcgctt
    27961 ttttgctatt caggcatcct caatttcact ttgtaaacct gatgacatcg tcagagctta
    28021 ctgtgcaagc aactctacgt cggtggaatt aggcgtaatt cgagtgactt ttccccacca
    28081 ttcgactatc ttgtttagca tataaaacaa attacgccga tagtagcgaa tattacgcta
    28141 atgtcggttt taacgttaag cctgtaaaac gagatggtaa gatgagcgtg atgtttgatc
    28201 cagacacggc gatttatcct ttccccccga agccgacgcc gttaagcatt gatgaaaaag
    28261 cgtattaccg cgagaagata aaacgtctgc taaaagaacg taatgcggtg atggttgccc
    28321 actactatac cgaccccgaa attcaacaac tggcagaaga aaccggtggc tgtatttctg
    28381 attctctgga aatggcgcgc ttcggtgcaa agcatcccgc ttctacgttg ttagtcgctg
    28441 gggtgagatt tatgggagaa accgccaaaa ttctcagtcc ggaaaaaaca attctgatgc
    28501 tgacacttca ggctgaatgt tcactggatc tcggctgccc tgttgaagaa tttaacgcat
    28561 tttgcgatgc ccatcccgat catactgtcg tcgtctacgc caacacttct gctgcggtaa
    28621 aagcgcgcgc agattgggtg gtaacttcaa gcattgccgt cgaacttatt gatcatctgg
    28681 atagttttgg tgaaaaaatc atctgggcac ccgacaaaca tctggggcgt tacgtgcaaa
    28741 aacagacggg cgcagacatt ttatgctggc agggtgcctg tattgtgcat gatgaattta
    28801 agactcaggc gttaacccgc ttgcaagaag aatacccgga tgctgccata ctggtgcatc
    28861 cagaatcacc acaagctatt gtcgatatgg cggatgcggt cggttccacc agtcaactga
    28921 tcgctgctgc gaaaacattg ccacatcagc ggcttatcgt ggcaaccgat cggggcattt
    28981 tctacaaaat gcagcaggcg gtgccagata aagagttact ggaagcacca accgcaggtg
    29041 agggtgcaac ctgccgca
//
LOCUS       DADMKF010000039        28814 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.040, whole genome shotgun sequence.
ACCESSION   DADMKF010000039 DADMKF010000000
VERSION     DADMKF010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28814
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.040"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(406..612)
                     /locus_tag="JNC24_001566"
     CDS             complement(406..612)
                     /locus_tag="JNC24_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HAY9158335.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            925..1014
                     /gene="kdpF"
                     /locus_tag="JNC24_001567"
     CDS             925..1014
                     /gene="kdpF"
                     /locus_tag="JNC24_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HAY9158336.1"
                     /translation="MSAGVIAGVLLVFLLLGYLVYALINAEAF"
     gene            1014..2687
                     /gene="kdpA"
                     /locus_tag="JNC24_001568"
     CDS             1014..2687
                     /gene="kdpA"
                     /locus_tag="JNC24_001568"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HAY9158337.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTAGVER
                     ILFRLPGVSDHEMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFAFIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGALQNFLPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPGP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            2710..4758
                     /gene="kdpB"
                     /locus_tag="JNC24_001569"
     CDS             2710..4758
                     /gene="kdpB"
                     /locus_tag="JNC24_001569"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HAY9158338.1"
                     /translation="MSRKQLALFEPTLVVQALKEAVKKLNPQAQWRNPVMFIVWIGSL
                     LTTCISIAMASGAMPGNALFSAAISGWLWVTVLFANFAEALAEGRSKAQANSLKGVKK
                     TAFARKLREPKYGAAADKVPADQLRKGDIVLVEAGDIIPCDGEVIEGGASVDESAITG
                     ESAPVIRESGGDFASVTGGTRILSDWLVIECSVNPGETFLDRMIAMVEGAQRRKTPNE
                     IALTILLIALTIVFLLATATLWPFSAWGGNAVSVTVLVALLVCLIPTTIGGLLSAIGV
                     AGMSRMLGANVIATSGRAVEAAGDVDVLLLDKTGTITLGNRQASEFIPAQGVDEKTLA
                     DAAQLASLADETPEGRSIVILAKQRFNLRERDVQSLHATFVPFTAQSRMSGINIDNRM
                     IRKGSVDAIRRHIEANGGHFPADVDQKVDQVARQGATPLVVVEGSRVLGVIALKDIVK
                     GGIKERFAQLRKMGIKTVMITGDNRLTAAAIAAEAGVDDFLAEATPEAKLALIRQYQA
                     EGRLVAMTGDGTNDAPALAQADVAVAMNSGTQAAKEAGNMVDLDSNPTKLIEVVHIGK
                     QMLMTRGSLTTFSIANDVAKYFAIIPAAFAATYPQLNALNIMRLHSPDSAILSAVIFN
                     ALIIVFLIPLALKGVSYKPLTASAMLRRNLWIYGLGGLLVPFIGIKVIDLLLTVCGLV
                     "
     gene            4767..5339
                     /gene="kdpC"
                     /locus_tag="JNC24_001570"
     CDS             4767..5339
                     /gene="kdpC"
                     /locus_tag="JNC24_001570"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HAY9158339.1"
                     /translation="MSGLRPALSTFLFLLLITGGVYPLLTTALGQWWFPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKLIAARV
                     AALRAANPDASASIPVELVTASASGLDNNITPQAAAWQIPRIAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            5332..8016
                     /gene="kdbD"
                     /locus_tag="JNC24_001571"
     CDS             5332..8016
                     /gene="kdbD"
                     /locus_tag="JNC24_001571"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdbD"
                     /protein_id="HAY9158340.1"
                     /translation="MNNEPLRPDPDRLLEQTAAPHRGKLKVFFGACAGVGKTWAMLAE
                     AQRLRAQGLDIVVGVVETHGRKDTAAMLEGLAVLPPKRLAYRGRHICEFDLDAALARR
                     PALILMDELAHSNAPGSRHPKRWQDIEELLEAGIDVFTTVNVQHLESLNDVVSGVTGI
                     QVRETVPDPFFDAADDVVLVDLPPDDLRQRLKEGKVYIAGQAERAIEHFFRKGNLIAL
                     RELALRRTADRVDEQMRAWRGHPSEEKVWHTRDAILLCIGHNTGSEKLVRAAARLASR
                     LGSVWHAVYVETPALHRLPEKKRRAILSALRLAQELGAETATLSDPAEEKAVVRYARE
                     HNLGKIILGRPASRRWWRRETFADRLARIAPDLDQVLVALDEPPARTINNAPDSRSFK
                     DKWRVQIQGCVVAAALCAVITLIAMQWLMAFDAANLVMLYLLGVVVVALFYGRWPSVV
                     ATVINVVSFDLFFIAPRGTLAVSDVQYLLTFAVMLTVGLVIGNLTAGVRYQARVARYR
                     EQRTRHLYEMSKALAVGRSPQDIAATSEQFIASTFHARSQVLLPDDNGKLQPLTHPQG
                     MTPWDDAIAQWSFDKGLPAGAGTDTLPGVPYQILPLKSGEKTYGLVVVEPGNLRQLMI
                     PEQQRLLETFTLLVANALERLTLTASEEQARMASEREQIRNALLAALSHDLRTPLTVL
                     FGQAEILTLDLASEGSPHARQASEIRQHVLNTTRLVNNLLDMARIQSGGFNLKKEWLT
                     LEEVVGSALQMLEPGLSSPINLSLPEPLTLIHVDGPLFERVLINLLENAVKYAGAQAE
                     IGIDAHFEGENLQLDVWDNGPGLPPGQEQTIFDKFARGNKESAVPGVGLGLAICRAIV
                     DVHGGTITAFNRPEGGACFRVTLPQQTAPELEDFHEDM"
     gene            8013..8690
                     /gene="kdpE"
                     /locus_tag="JNC24_001572"
     CDS             8013..8690
                     /gene="kdpE"
                     /locus_tag="JNC24_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HAY9158341.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVFEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSPVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPDPLVKFSDVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPRHFITETG
                     IGYRFML"
     gene            8965..9069
                     /locus_tag="JNC24_001573"
     CDS             8965..9069
                     /locus_tag="JNC24_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2618 domain-containing protein"
                     /protein_id="HAY9158342.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            9380..11578
                     /gene="speF"
                     /locus_tag="JNC24_001574"
     CDS             9380..11578
                     /gene="speF"
                     /locus_tag="JNC24_001574"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
                     /protein_id="HAY9158343.1"
                     /translation="MSELKIAVSRSCPDCFSTHRACVNIDESNYIDVAAIILSVNDVE
                     RGKLDEIDATGYGIPVFIATENEERVPAEYLPRISGVFEHCESRKEFYGRQLETAASH
                     YETQLRPPFFRALVDYVNQGNSAFDCPGHQGGEFFRRHPAGNQFVEYFGETLFRSDLC
                     NADVAMGDLLIHEGAPCIAQQHAAKVFNADKTYFVLNGTSSSNKVVLNALLTPGDLVL
                     FDRNNHKSNHHGALLQAGATPVYLETARNPYGFIGGIDAHCFEESYLRELIAEVAPQR
                     AKEARPFRLAVIQLGTYDGTIYNARQVVDKIGHLCDYILFDSAWVGYEQFIPMMADCS
                     PLLLDLNENDPGILVTQSVHKQQAGFSQTSQIHKKDSHIKGQQRYVPHKRMNNAFMMH
                     ASTSPFYPLFAALDINAKMHEGVSGRNMWMDCVVNGINARKLILDNCQHIRPFVPELV
                     DGKPWQSYETAQIAVDLRFFQFVPGEHWHSFEGYAENQYFVDPCKLLLTTPGIDARNG
                     EYEAFGVPATILANFLRENGVVPEKCDLNSILFLLTPAEDMAKLQQLVALLVRFEKLL
                     ESDAPLAEVLPSIYKQHEERYAGYTLRQLCQEMHDLYARHNVKQLQKEMFRKAHFPHV
                     SMNPQEANYAYLRGEVELVRLPDAEGRIAAEGALPYPPGVLCVVPGEIWGGAVLRYFS
                     ALEEGINLLPGFAPELQGVYIEEHDGRKQVWCYVIKPRDAQSTLLKGEKL"
     gene            11575..12894
                     /gene="potE"
                     /locus_tag="JNC24_001575"
     CDS             11575..12894
                     /gene="potE"
                     /locus_tag="JNC24_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HAY9158344.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFMANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGVIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(12950..14590)
                     /gene="pgm"
                     /locus_tag="JNC24_001576"
     CDS             complement(12950..14590)
                     /gene="pgm"
                     /locus_tag="JNC24_001576"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-glucose phosphate-specific
                     phosphoglucomutase"
                     /protein_id="HAY9158345.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALMADGLKGVKRISLDEAMASGHVKEQDLVQQFVEGLADI
                     VDMAAIQKTGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(14616..15161)
                     /gene="seqA"
                     /locus_tag="JNC24_001577"
     CDS             complement(14616..15161)
                     /gene="seqA"
                     /locus_tag="JNC24_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HAY9158346.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            15346..16110
                     /gene="ybfF"
                     /locus_tag="JNC24_001578"
     CDS             15346..16110
                     /gene="ybfF"
                     /locus_tag="JNC24_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HAY9158347.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPRDPVMNYPAMAQDLVDTLDAQQIDKATFIGHSMGGKAVMALTALA
                     RDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMHQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            16181..16543
                     /gene="ybfE"
                     /locus_tag="JNC24_001579"
     CDS             16181..16543
                     /gene="ybfE"
                     /locus_tag="JNC24_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HAY9158348.1"
                     /translation="MYYGALSIRAEAWLIVSPEVTKIMAKEQTDRTTLDLFAHERRPG
                     RPKTNPLSRDEQLRINKRNQLKRDKVRGLKRVELKLNAEAVEALNELAESRNMSRSEL
                     IEEMLMQQLAALRSQGIV"
     gene            16683..17213
                     /gene="fldA"
                     /locus_tag="JNC24_001580"
     CDS             16683..17213
                     /gene="fldA"
                     /locus_tag="JNC24_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HAY9158349.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            17423..17509
                     /gene="uof"
                     /locus_tag="JNC24_001581"
     CDS             17423..17509
                     /gene="uof"
                     /locus_tag="JNC24_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HAY9158350.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            17502..17948
                     /gene="fur"
                     /locus_tag="JNC24_001582"
     CDS             17502..17948
                     /gene="fur"
                     /locus_tag="JNC24_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HAY9158351.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(18033..18359)
                     /locus_tag="JNC24_001583"
     CDS             complement(18033..18359)
                     /locus_tag="JNC24_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9158352.1"
                     /translation="MKKLILIAIMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQHFADQESVRVLDYQQYLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(18409..19815)
                     /gene="chiP"
                     /locus_tag="JNC24_001584"
                     /pseudo
     CDS             complement(18409..19815)
                     /gene="chiP"
                     /locus_tag="JNC24_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
     gene            complement(20393..22057)
                     /gene="glnS"
                     /locus_tag="JNC24_001585"
     CDS             complement(20393..22057)
                     /gene="glnS"
                     /locus_tag="JNC24_001585"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HAY9158353.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVESIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(22316..24262)
                     /gene="nagE"
                     /locus_tag="JNC24_001586"
     CDS             complement(22316..24262)
                     /gene="nagE"
                     /locus_tag="JNC24_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HAY9158354.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVVFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLTQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDAMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVSDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLIIKAQGNVVAGQTPLYEIKK"
     gene            24595..25395
                     /gene="nagB"
                     /locus_tag="JNC24_001587"
     CDS             24595..25395
                     /gene="nagB"
                     /locus_tag="JNC24_001587"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HAY9158355.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            25455..26603
                     /gene="nagA"
                     /locus_tag="JNC24_001588"
     CDS             25455..26603
                     /gene="nagA"
                     /locus_tag="JNC24_001588"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HAY9158356.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLARHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIGVEKRLGTLAAGKVANLTSFTPDFKITKTIVNGNEVVTQ"
     gene            26612..27832
                     /gene="nagC"
                     /locus_tag="JNC24_001589"
                     /gene_synonym="nagR"
     CDS             26612..27832
                     /gene="nagC"
                     /locus_tag="JNC24_001589"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HAY9158357.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            27880..28632
                     /gene="nagD"
                     /locus_tag="JNC24_001590"
     CDS             27880..28632
                     /gene="nagD"
                     /locus_tag="JNC24_001590"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HAY9158358.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETRSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
BASE COUNT         6751 a         7353 c         7560 g         7150 t
ORIGIN      
        1 gcaccgagca ggggattgac cggatggccg gacgtcactc cgccggggca caccgaacag
       61 gccacaccgg tgggggcacc gatgcgcacc ccggctgaac cctggacaat gctaccgcca
      121 tactgcgtca tgtcgccctg acgcgccgcc ggttttccgc tcatttctca tccttgtttt
      181 atcgggttat ttgtccgctg acaggcaaga ccctacagat ttataagaaa gccttaagtg
      241 agatatccgt cagatagaaa caattaggaa aatatttata tatatttcat gaatatatgt
      301 ttcgattggt ataacggtgt ctaaatattc ctctgaacat cttcctgata caaaaaaccg
      361 ccagcgtttc cgctggcggt tttcaatgta ttcagaactc agcaatcagt aaaaatcacc
      421 ggttgccttt tcggcctgat ccatccacac cggtttatcg ctggttttcg accagacgcg
      481 gtgcaggtag ctgtaaaagc gggcgcggtc tttccagaaa agcatgaaag gcagcgccag
      541 aacgcccgct gcaaccgcat atgtacggcg taaaaagata agccatgcag gatattctct
      601 gtagagttcc atacttttct cccccttttg tatctacccg gtgaatagca ccggaaaaat
      661 gaatttgttt atctgatgaa aatagtaccg ccttttgtgt aattttacta ctcatccgac
      721 cacttatttt tgcttattga tggtttattt acattcactc tgtaattaag ttacacaaaa
      781 gttaaattaa tactaaacat tagttaaatc atggcttttg ccatttttat acttttttta
      841 caccccgccc gcagattttt gcgaaatctt tgcagccaga attctactct tccggtatca
      901 cttttaggcc actggaggtg cactgtgagt gcaggcgtga tagccggcgt gttgctggtg
      961 tttttattac tgggttattt ggtttatgcc ctgatcaatg cggaggcgtt ctgatggctg
     1021 cgcaagggtt cttactgatt gccacgtttt tactggtatt aatggtgctg gcgcgtcctt
     1081 taggcagcgg gctggcgcgg ctgattaatg acattcctct tccaggtaca gcgggcgttg
     1141 aacgtatact ctttcgttta cctggcgtct ctgaccatga gatgaactgg aagcaatatc
     1201 tttgtgccat tctcggcctg aacatgctgg ggctggcggt gctgtttttt atgttgctcg
     1261 gtcagcacta tctgccgctt aatccacagc agttgccggg gctgtcgtgg gatctggcgc
     1321 tgaataccgc cgtcagcttt gtcacgaata ccaactggca atcttatagc ggtgaaacca
     1381 cgttgagcta tttcagccag atggcaggct taacggtaca aaactttctt tctgccgcca
     1441 gcgggattgc ggtgattttt gccttcatcc gtgcgtttac tcgccagagc atgagcacac
     1501 tcgggaatgc ctgggtcgat ctgctacgca tcacgttatg ggtgctagtc cctgtggcgt
     1561 tgttgatcgc actgtttttt attcaacaag gtgcgctgca aaactttctg ccctatcagg
     1621 cagtgaatac cgttgaagga gcgcaacagc tgttacccat gggaccagta gcttctcagg
     1681 aagcgatcaa gatgctcggt actaacggcg gtggcttctt taatgccaac tcgtcgcatc
     1741 cgtttgaaaa cccaaccgca ctgaccaact tcgtgcagat gctggcgatc ttcttgatcc
     1801 caacagcgct gtgctttgcc tttggtgaag tgacgggcga tcgccgccag gggcgcatgt
     1861 tgctgtgggc gatgtcagtg atttttgtca tctgcgtagg cgtggttatg tgggcagaag
     1921 ttcagggtaa tcctcatctg ctggcgctcg gcgcagacag cagcatcaat atggaaggta
     1981 aagagagccg tttcggcgtg ctggtcagta gcctgtttgc ggtcgtgact acggcggcgt
     2041 cctgtggcgc ggtgattgcg atgcatgatt cgtttaccgc tctcggcggc atggtgccga
     2101 tgtggctaat gcaaattggt gaagtggtgt tcggcggtgt cggttctggt ctttacggca
     2161 tgatgctgtt tgtcctgctg gcggtgttta ttgccgggct gatgattggt cgtacaccgg
     2221 aatatctggg taaaaaaatc gacgtacgcg agatgaaact gactgcactg gcgattctgg
     2281 ttactccaac actggtactg atgggcgcgg cgttggcgat gatgaccgac gccggacgta
     2341 gcgccatgct taaccctggc ccgcatggtt ttagcgaagt gctgtacgcc gtgtcgtccg
     2401 ccgctaacaa caacggcagc gcctttgccg gattaagcgc caactctccg ttctggaact
     2461 gtttactggc gttctgcatg tttgtcggtc gcttcggggt gattatcccg gtgatggcaa
     2521 ttgccggttc gctggtgagt aaaaagagcc aacccgccag ctccggcacg ctgccaacgc
     2581 acggcccgct gtttgttggc ctgttaatcg gcaccgtgtt gctggttggc gcactgacct
     2641 ttatccctgc cctggcgctt ggtccggtgg cggaatatct ctcctgatga tattgagcga
     2701 gcactgaata tgagtcgtaa acaactggcg ctattcgaac caacacttgt cgttcaggcg
     2761 ctgaaagaag cggtgaaaaa attaaacccg caggcgcaat ggcgcaatcc ggtgatgttt
     2821 atcgtctgga tcggcagtct gctgaccacc tgtattagca tcgcgatggc aagcggtgcg
     2881 atgcctggca atgcgctgtt tagcgcggca attagcggct ggctgtgggt caccgtactg
     2941 ttcgctaatt tcgccgaggc gctggcagaa ggccgcagta aagcgcaggc caacagtctt
     3001 aaaggggtga aaaaaactgc ctttgcccgc aagctgcgtg agccgaaata tggcgctgcg
     3061 gcggacaaag ttcctgccga tcaactgcgt aaaggtgaca tcgtgctggt agaagctggc
     3121 gatattatcc cctgcgatgg tgaagtcatt gaaggcggcg catcggtcga tgaaagcgcc
     3181 atcaccgggg aatcggcacc ggtgatccgt gaatccggcg gcgattttgc ctccgtcacc
     3241 ggcggcacgc gtattctttc tgactggctg gtgattgagt gtagcgttaa ccccggtgag
     3301 acatttctgg atcggatgat cgcgatggtg gaaggcgcac agcgacgcaa aacaccgaac
     3361 gagattgccc tgactatcct gctgattgct ctgaccatcg tctttttact ggcaaccgcc
     3421 acgctgtggc cgttttccgc gtggggcggt aatgccgtga gtgtaacggt actggtggcg
     3481 ctgctggtct gtctgatccc aaccactatt ggcggcctgt tgtcagcgat cggcgtcgcc
     3541 gggatgagtc ggatgctggg cgcgaatgtg attgccacca gcggacgtgc agttgaagcg
     3601 gcaggtgacg ttgacgttct gctactggat aaaaccggca ccatcacact cggtaaccgt
     3661 caggcgtcgg agtttatccc cgcgcagggc gtggatgaaa aaacgctggc tgacgccgca
     3721 caactggctt cgctggctga tgaaacgccg gaaggtcgca gtattgtgat cctcgccaag
     3781 caacgtttta acctgcgcga gcgcgatgtg cagtcgctcc atgccacctt tgtaccgttt
     3841 actgcgcaaa gccggatgag cgggatcaac atcgacaacc gcatgatccg taaaggttct
     3901 gtcgatgcca ttcgccgcca tattgaggct aacggcggtc acttccctgc cgatgttgat
     3961 caaaaagtcg atcaggttgc gcgtcaggga gccacgccgc tggtggtggt ggaaggttct
     4021 cgtgtgctgg gcgttattgc gctgaaagat atcgtcaaag gcggtattaa agagcgcttc
     4081 gcccagctgc gcaaaatggg cattaaaacg gtgatgatta ccggcgataa ccgtctgact
     4141 gccgccgcga tagctgcgga agcgggtgtc gatgattttc tcgccgaagc aacaccggag
     4201 gccaagctgg cattgatccg tcagtatcag gcggaaggtc gtttggtagc gatgaccggc
     4261 gacggcacca acgatgctcc ggcgctggcg caggcagatg tcgcggtggc gatgaactcc
     4321 ggcacccagg cggcgaaaga ggcgggcaat atggtggatc tcgactctaa cccgaccaag
     4381 ttgatcgagg tggtgcacat tggcaaacag atgctgatga cccgtggctc gctgaccacc
     4441 ttcagcattg ccaacgatgt ggcgaaatac ttcgccatta ttccggcggc gttcgcggca
     4501 acatatccgc agttaaatgc gctgaacatc atgcgcctgc attcgcccga ctccgccatt
     4561 ctcagtgcgg tcattttcaa cgccttgatt atcgtctttt tgattcctct ggcgttaaaa
     4621 ggcgtgagtt ataaaccgct taccgcttct gccatgttgc gccgtaactt atggatttac
     4681 ggtctgggtg ggctgctggt gccgtttatt ggtatcaaag tcattgattt actgctgacc
     4741 gtttgcggtc tggtgtgagg tttaccatga gtggattacg tccggcatta tcgacatttc
     4801 tctttctgtt attgattact ggcggcgttt acccgctgct gaccaccgca ctggggcaat
     4861 ggtggtttcc ctggcaggcc aacggttcgt tgatccgtga aggtgatacg gtgcgtggct
     4921 cggcattaat cgggcagaat tttaccggca acggctattt tcatggtcgc ccgtcagcaa
     4981 cggctgaaat gccctataat ccacaggctt ctggcgggag caatctggcg gtcagtaacc
     5041 ctgagctgga taaactaata gccgcacgcg ttgctgcatt acgggccgct aacccggatg
     5101 ccagcgcgag cattccggtt gaactggtga cggcatcggc aagcgggctg gacaataata
     5161 tcaccccgca agcggcggcc tggcagatcc cacgcatcgc gaaagcgcgt aatctcagcg
     5221 ttgaacagct cacgcagctg atcgcaaaat acagccaaca accgctggtg aaatatatcg
     5281 gccagccggt cgtcaacatt gtcgaactca atctggcgct ggataagctt gatgaataac
     5341 gaacccttac gtcccgaccc cgatcgtctg ctggaacaaa ctgccgcgcc gcatcggggg
     5401 aagctgaaag ttttcttcgg tgcctgtgca ggcgtcggga agacctgggc gatgctggca
     5461 gaagcccagc gactgcgggc gcaagggctg gatattgtgg ttggcgtggt agaaacccac
     5521 gggcgaaaag ataccgccgc aatgctggaa gggctggctg ttctgccgcc aaaacgcctg
     5581 gcgtaccgtg ggcggcacat ctgcgagttt gacctcgatg ccgccctcgc ccgccgtccg
     5641 gcgctgatct taatggacga actggcgcac agtaatgcgc caggttctcg tcaccctaaa
     5701 cgctggcagg acatcgaaga gctgctggaa gcaggcattg atgttttcac taccgtcaac
     5761 gttcagcatc tggaaagtct gaatgatgtg gtcagcggcg tcaccggaat tcaggtacgg
     5821 gaaaccgtgc ccgatccttt tttcgatgcc gccgacgacg tggttctggt ggacttgccc
     5881 ccggacgatc tgcgccagcg gctgaaagaa ggcaaagtct atattgccgg gcaggcggag
     5941 cgcgccattg aacatttttt ccgcaaaggc aatctgatcg ccctgcgcga actggcactg
     6001 cgccgtactg ccgatcgcgt tgatgagcaa atgcgcgcct ggcgggggca tcctagcgaa
     6061 gagaaagtct ggcacacgcg cgacgcgatc cttttatgca tcggccataa caccggtagt
     6121 gagaagctgg tccgcgcggc ggcgcggctg gcgtcacggc tgggtagcgt ctggcacgcg
     6181 gtgtatgttg aaacccctgc cctgcatcgc ttaccggaga aaaaacgtcg tgcaattctc
     6241 agcgccttac gtctggcgca ggaactgggc gcggagacgg caacactttc tgatccagcg
     6301 gaagagaaag cggtagtgcg ttatgcccgt gaacataatc tcggcaagat tattctcggt
     6361 cgcccggcct cgcgccgctg gtggcgtcgg gaaacgtttg ctgaccgact ggcgcgcatc
     6421 gcccccgatc tcgatcaggt gctggtcgcg cttgatgaac cacccgcccg cacgattaac
     6481 aacgcgccgg atagccgctc ttttaaagac aagtggcggg tacagattca gggatgcgtg
     6541 gttgccgccg cgttatgcgc cgttatcacc ttaattgcca tgcagtggct gatggcgttt
     6601 gatgccgcca acctggtgat gctgtatctg cttggcgtgg tggtggtggc gctattttat
     6661 ggacgctggc cttcggtggt tgccaccgtc attaatgtgg tgagtttcga tctctttttt
     6721 atcgccccac gcggcacgct cgccgtctct gatgtgcaat atctgctgac cttcgcggtg
     6781 atgttaaccg tcgggctggt gatcgggaac cttactgctg gcgtgcgtta tcaggcgcgg
     6841 gtagcccgtt accgcgagca acgcacacgg cacttatatg aaatgtcgaa agctctggcg
     6901 gtgggccgca gtccgcagga catcgccgcc accagcgaac aatttattgc ctccacgttt
     6961 catgcccgca gtcaggtgtt gttgcccgat gacaacggta aattgcagcc gttaacacat
     7021 ccgcaaggaa tgacgccatg ggacgatgcc atcgcgcagt ggagttttga taaaggcctg
     7081 cctgcgggcg cgggcaccga cacgttaccc ggtgtaccgt accagatttt gccgctaaaa
     7141 agcggcgaga aaacctacgg gctggtggtg gtggagccgg ggaatctgcg ccagttgatg
     7201 atcccggaac agcagcgcct gctggagacg tttacgctgt tagtcgccaa tgcccttgag
     7261 cggctgacgc taaccgccag cgaagaacag gcgcggatgg caagcgaacg tgaacagatc
     7321 cgcaacgccc tgctggcagc gctttcgcat gatttacgca cgccgcttac ggtgctgttt
     7381 ggtcaggcag aaatcttaac gctcgatctg gcaagcgaag gatcacccca cgcccgccag
     7441 gccagcgaga ttcgtcagca tgtgctgaac actacccgac tggtgaataa tctactggat
     7501 atggcgcgaa ttcagtccgg cggctttaat ttgaagaaag agtggttaac gctggaagaa
     7561 gtagtcggca gcgcgctgca aatgctggaa ccgggtttat cgtcgcccat caatctttct
     7621 ctgccagaac cgctgacctt aatccacgtt gacgggccac tctttgaacg ggtgctgatt
     7681 aatctgctgg agaacgcggt gaaatatgcg ggtgcgcagg ccgaaattgg tatcgatgcc
     7741 cactttgagg gcgaaaatct acaactggat gtctgggata acggccccgg tcttccgcca
     7801 ggccaggagc agacgatatt tgataagttt gctcgcggga ataaagagtc ggcggtgccg
     7861 ggtgtcgggc ttgggttggc aatttgtcgg gcgattgtgg atgtacacgg cggcaccatt
     7921 accgcgttca accgaccgga aggtggtgcc tgttttcgtg ttacacttcc ccagcaaacc
     7981 gctcctgaac ttgaagactt tcatgaggat atgtgacaaa cgttctgatt gttgaagatg
     8041 aacaggctat tcgtcgcttt ctgcgcacgg cgctggaggg cgacgggatg cgcgtctttg
     8101 aggccgaaac tctgcaacgc ggcttgctgg aagcggcaac ccgtaagcca gatttgatta
     8161 ttctcgatct cggcctgccc gatggtgatg ggattgagtt tatccgcgac ctgcgccagt
     8221 ggagcccggt gccggtgatt gtgctttccg cacgcagcga agagagcgac aagatcgccg
     8281 cgctggatgc cggagcggat gattatctga gtaagccgtt tggcattggc gaattgcagg
     8341 cccgtctgcg cgtcgcttta cgccgccact ccgccaccgc cgcgcccgat ccgctggtga
     8401 aattttccga tgttaccgtc gatttagccg cccgcgtgat tcaccggggt gaggaagagg
     8461 tgcatctcac accaattgag ttccgcctgc tggcggtgct gctcaacaat gccggaaaag
     8521 tactcaccca gcgccagctc cttaaccagg tgtgggggcc aaacgcggtc gaacacagtc
     8581 actatttgcg tatttatatg ggacatctgc gacaaaaact ggaacaggat ccggcccggc
     8641 ctcgccactt cattactgaa accggtattg gatatcggtt tatgctttga attgttattc
     8701 aataaattcg cttaaatagt gattaattta ttattaaacc tgcgccgaat attattattt
     8761 ggcaggttta atttcttaac gcaaatataa aacacgaaat tacaacattt aagtaacaaa
     8821 acagcatcta atatggcgat attcataatt aatgatttat tatctggggt atgatcgttt
     8881 tttgttgatc ttcttcacag attatagcca tttcatggat agaataactc taccttcaac
     8941 tgacacagca agaggtaaag gtaaatggaa aataataacc gcttaatgcc tcatataagg
     9001 cggacaaccc atatcatgaa gtttgcccat cgtaatagtt ttgactttca tttctttaat
     9061 gcccgctagt cttctgacta aagggcaccc caacgtacag gtctccctga ctttaagcat
     9121 tacaggttaa tacctgtatt cctcggtgct catatactgc taaccctttt aaactctaaa
     9181 taattcgagt cgcagcactt gcaacttgag gtatgacgag tatagccagt taccgggctg
     9241 gtctgggtta ttgcatctgc aaaaagcaaa ctactgattt atttatcagc ggtggagctt
     9301 tgcttttttt ccggcgtgat cgatttctcc tttgagaaat tgaggacctg ctattacctg
     9361 aaataaagag atgaataaaa tgtcagaatt aaaaattgcc gttagccgtt cttgcccgga
     9421 ttgtttttcc actcatcgtg catgcgtgaa tattgacgaa agtaattata ttgacgttgc
     9481 cgccattatt ttatcagtca atgatgttga acgtggaaaa ctcgatgaaa tagacgctac
     9541 tggctatggc attcctgttt ttattgcaac ggaaaatgaa gaacgtgtac cagcagaata
     9601 tctgccacgt atttctggtg tcttcgagca ttgcgaatca cggaaagagt tttatggacg
     9661 tcagttagaa accgctgcta gccattatga aacgcaactg cgcccgccct tcttccgcgc
     9721 actggtcgat tatgtcaatc agggcaatag cgcttttgac tgcccgggtc atcagggagg
     9781 tgagtttttc cgtcgtcatc cggcagggaa tcagttcgtg gagtacttcg gagaaacgct
     9841 gttccgttcc gacttatgca acgcagacgt agcaatgggc gatctgctga ttcacgaagg
     9901 cgcgccatgc attgcacagc aacatgcggc aaaagtgttt aatgccgata aaacatactt
     9961 cgttctaaat ggcacttcat cttctaacaa agtggtttta aacgccctgc taacaccggg
    10021 tgatctggtg ctgtttgatc gcaataacca caaatctaac caccacggag cgttgctaca
    10081 ggctggtgca acaccggttt atctggaaac ggcacgtaac ccgtatggct ttatcggtgg
    10141 cattgatgcg cactgttttg aagaaagtta cctgcgtgag ctgatcgcgg aagtcgcacc
    10201 gcagcgggca aaagaggctc gtcctttccg cctcgctgtg attcagttag gcacctacga
    10261 cggtacgatt tataacgccc gccaggtggt ggataaaatt ggtcatctgt gtgactacat
    10321 tctgtttgac tccgcatggg ttggctatga gcaatttatc ccgatgatgg ctgactgttc
    10381 gccgctgttg ctggatctta atgagaacga tccgggtatt ctggttacgc aatccgtaca
    10441 taaacagcag gctggttttt ctcaaacttc acaaatccat aaaaaagaca gccacatcaa
    10501 agggcaacag cgttatgtac cgcacaaacg catgaacaac gcctttatga tgcacgcctc
    10561 caccagcccg ttctatccgc tgtttgccgc actggatatc aacgccaaaa tgcatgaagg
    10621 tgtcagcggt cgtaatatgt ggatggattg tgtggtaaat ggcattaatg cccgcaaact
    10681 gatcctcgat aactgtcagc atattcgtcc gttcgtacct gaactggtgg atggtaaacc
    10741 ctggcagtcg tatgaaacag cgcaaattgc ggttgatctg cgcttcttcc agtttgtacc
    10801 aggggaacac tggcattctt ttgaaggcta tgcagagaat caatactttg tcgatccttg
    10861 caaactgttg ctgacaaccc caggtattga tgcacgtaac ggcgaatatg aagcgttcgg
    10921 tgtacccgcg acgattcttg ctaacttcct gcgcgaaaat ggcgtagtgc cggaaaaatg
    10981 cgatcttaac tccatcctct tcctgctgac tccggcagaa gatatggcca aacttcagca
    11041 acttgttgcc ctgctggtac gcttcgaaaa actgcttgag tccgacgcgc cattagcaga
    11101 agtgctgcct tccatctaca aacagcatga agagcgctac gccggttata ccctgcgtca
    11161 gttgtgccag gaaatgcatg atttgtatgc ccgccacaac gtgaaacaac tgcaaaaaga
    11221 gatgttccgc aaagcgcact tcccacacgt cagcatgaat ccgcaagaag ccaactacgc
    11281 ctatttacgc ggtgaagtgg agctggttcg tctgccggat gccgaaggtc gtatcgctgc
    11341 cgaaggggcg cttccttatc ctccgggtgt gctgtgtgtt gttccgggtg aaatctgggg
    11401 tggtgctgtt ttgcgttact tcagcgctct ggaagaaggg atcaacctgc tgccaggttt
    11461 tgcaccggag ctgcagggtg tctatatcga agaacatgat ggtcgtaagc aagtttggtg
    11521 ctatgtcatc aagcctcgtg atgcgcaaag caccctgttg aaaggggaaa aattatgagt
    11581 caggctaaat cgaacaaaat gggcgtcgtt cagttaacca tactgacgat ggtcaacatg
    11641 atgggctccg gtatcatcat gctgccgaca aagcttgccg aagttgggac aatttcaatt
    11701 atctcctggc tggtgacagc cgtaggctca atggcactgg catgggcatt cgcaaaatgc
    11761 ggtatgttca gccgtaaatc aggtggtatg ggcggttatg ccgagtatgc tttcggtaaa
    11821 tccggtaact ttatggcgaa ctatacctac ggcgtctcac tgctgattgc taacgtcgcg
    11881 attgctattt cggcggttgg ttacggcacc gaactgctcg gcgcgagttt gtcgccagtg
    11941 cagattggtc ttgcgaccat cggggtgctg tggatttgta ccgtggctaa ctttggtggt
    12001 gcgcgcatta ccgggcaaat cagtagcatt accgtgtggg gggtcattat tccggtggta
    12061 ggcctgtgca ttatcggctg gttctggttt agcccgacgc tttacgttga ttcctggaat
    12121 ccgcatcatg caccgttctt cagtgcagta ggttcttcca tcgctatgac gctgtgggct
    12181 tttctcggtc tggagtctgc gtgtgcgaat actgatgtag tggaaaaccc ggaacgtaat
    12241 gtgccaatcg cggtactcgg cggtacgtta ggtgcggcgg tgatttatat cgtctccacc
    12301 aacgtgattg cagggattgt gcctaatatg gagctggcaa attcaacggc accatttggc
    12361 ctggccttcg cacagatgtt cacaccagaa gtgggtaaag tcattatggc gctgatggtg
    12421 atgtcctgct gcggttcgct gcttggctgg cagttcacca ttgcccaggt gtttaagtct
    12481 tcatctgatg aaggctactt ccctaaaatt ttctcccgtg taaccaaagt ggatgcaccg
    12541 gtgcagggaa tgttgaccat tgtgattatc cagagcgggt tggcgctgat gacgattagc
    12601 ccgtcgctaa acagtcagtt caacgtgctg gttaacctgg ccgtggtgac caatatcatt
    12661 ccgtatattc tgtcaatggc ggcattagtc attattcaga aggtcgctaa tgtgcccccc
    12721 tcaaaagcga aagttgcaaa ctttgttgct tttgttggcg cgatgtatag cttttatgcg
    12781 ctgtactcat ccggggaaga agccatgctg tacggttcca tcgtgacctt ccttggttgg
    12841 acactgtatg gtctggtatc accacgcttt gaactgaaaa ataaacacgg ttaaaaaagc
    12901 aaagtaaaaa agggcgatct tgcgaccgcc ctttttttat taaatgtgtt tacgcgtttt
    12961 tcagaacttc gctaacaatc tcaaccgctt ctttctcaat ctgcttgcga tgttcttcac
    13021 cgaggaagct ttcgcagtag atcttatatg cgtcttccgt gcctgacgga cgcgcggcga
    13081 accagccgtt gtcagtcatc actttcagac cgccgataga agcaccattg cccggtgccg
    13141 ccgtcaggcg cgcggtgatc gggtcgcctg ccagggtgct ggcgctcacc atttccggag
    13201 acagcttaga cagcgccgct ttttgtgcgg aagtcgcagc tgcctgcaaa cggttgtagc
    13261 tcggcgcacc aaagcgtttt gccagttcgt tgtagtgttc ctgcgggttc ttaccggtga
    13321 cagcggtgat ttccgccgcc agcagacaca tgatgatgcc gtctttgtcg gtggaccacg
    13381 gcgtgccgtc gaaacgcagg aaggaagccc ctgcgctctc ttcgccgcca aagccgaagc
    13441 tgccgtcgaa cagaccatca acaaaccatt tgaaacctac cgggacttct accagcttgc
    13501 ggcccaagtc attgaccaca cggtcgatca tcgcagaaga aaccagcgtt ttaccgacag
    13561 caacatcttt gccccactgc ggacgatgct ggaacaggta gttgatcgcc accgccaggt
    13621 agtggttcgg attcatcaaa cctgccggag tgacgatacc gtgacggtca taatccgggt
    13681 cgttagcaaa cgccagatcg aacttatcac gcaatgccag caggcccgcc atcgcacact
    13741 cggaggagca gtccatacgg atcgcgccgt ctttatcgag gtgcataaag cggaaggttt
    13801 gatcgacctg atcgttaacg atggtcaggt tgaggttgta atactcgcca attcgcttcc
    13861 agtattcgat accggaacca cccagcggat caacgcccag cgtcaggccc gttttctgaa
    13921 tcgccgccat atcaacgata tcggccagac cttccacaaa ctgctgcacc agatcctgct
    13981 ctttcacatg accggatgcc atcgcttcgt cgagggagat acgcttcacg cctttcaggc
    14041 catcggccat cagtgcgttg gcacgatctt ccaccacttt agtgacgttg gtatcagccg
    14101 ggccaccatt tggcggattg tacttgatac caccatcttc cggcgggtta tgggagggtg
    14161 taatcacgat accgtctgcc agcgggccac cttttttatt atgaaccagg atggcattgg
    14221 aaacggcagg cgtcggagtg aagccattgt tttcctgcac aatgacatca acgccgttcg
    14281 ctgccagcac ttccagtacg gaaatgaacg caggctcgga cagggcgtga gtatctttac
    14341 ccacatagca agggccagtg atgccgtttt tcgcacgttc ttcagcaatt gcctgagcga
    14401 tcgccagaat gtgcggctcg ttaaagctgt ggcgcgctgc actgccacgg tgaccggaag
    14461 taccgaattt caccgcgtgc tccgcattcc ctgcttctgg tttcagtaca taatattgcg
    14521 ccgtcagttg ggcgacgtta atcaaatcac tctgttgtgc aggttggcct gcacgattgt
    14581 ggattgccat tgctttgtcc tttgtctgca acgttttaga tagttccgca aaccttctca
    14641 atcaattccg ccgggaattg catcgactgc atgatgtgtt cgatcatgct gcatttacgg
    14701 ccggtgttgg tgttggtgat cacccaatac ggcgtgcctg gcacatgttt cggcttggtc
    14761 tgattaccat ttttcagcag cgtttgttca tctgccgcaa agtaaacgcg tgtacgaccg
    14821 tgcaacgatt ccgttgcttc ggcaaacgcc tgggcgtcaa gagaatatag tgtagacaac
    14881 agcagcataa agcgattgac cgctcgcttt tgctctgcgt attcgtcaga aagcagaagt
    14941 tcacgcattg cgcgaacctt gtctttaatc gttttgaccg gcttcgcttc gacgatagca
    15001 ggtgacgcaa cgcgaacctc tttcgtcacc ggagcagcag gctgtgatgc ggcggaaaat
    15061 ttcaacatac gccgtaaaat gtcggatgcg ctctcgccga tatgcttagt gtggctggca
    15121 atatagctgt agagttcatc atcaacttca atcgttttca tcttaatcca gtacagtgtc
    15181 ttagctgaat acaagtcgcc aagagtatat gaataaatcc cggcagcgga tagcgtcaag
    15241 cctggcttgc agaaaaactc ggaataaacc ttatttgttg cagccctgtg gcacaatggt
    15301 caacataata ccctaacccg acaacgcgaa aaaaagaact ttgccatgaa attgaatatc
    15361 cgcgcgcaaa ctgcacaaaa ccagcacaat aattctccca tcgttcttgt ccatggtctg
    15421 tttggtagcc tcgacaacct tggcgtactg gctcgcgatc tggtaaacga tcacaatatc
    15481 atccaggttg atatgcgtaa ccacggtctt tcaccgagag atccggtaat gaattacccg
    15541 gcgatggcgc aggatcttgt tgatactctg gatgcacagc agatcgacaa agcaacattt
    15601 atcggtcact ccatgggcgg taaagcggta atggcactta cggcactggc ccgcgatcgc
    15661 atcgataaac tggtggcgat cgatatcgca ccggtcgact atcacgtacg ccgtcatgat
    15721 gagattttcg cggctatcaa cgcggtcagt gaatcggacg cacaaactcg ccagcaagca
    15781 gcagcaataa tgcaccagca tcttaatgaa gaaggggtga ttcagtttct gttgaaatct
    15841 tttgttgacg gggagtggcg ctttaacgtg ccggtattgt gggatcagta tccgcatatt
    15901 gtgggttggg agaaaatccc ggcatgggat caccctgccc tgtttatccc tggcggcaat
    15961 tctccgtatg ttagcgagca gtaccgtgat gatttactgg cacaatttcc acaggcacgg
    16021 gcgcatgtga ttgcaggcgc gggtcactgg gtccatgctg aaaaaccgga tgcggtatta
    16081 cgcgctatcc gtcgctatct caatgattaa cgcaactgat taaaaaccca gcgtccgcgt
    16141 cgctttccgc gcgtgggcgt tggcgtgact catcgcgctg atgtattatg gcgcgctatc
    16201 catccgggca gaggcctggc tgattgtttc ccccgaagtc accaagatca tggccaaaga
    16261 acaaacggac cgtacgacat tagatctgtt cgcgcacgag cgtcgaccgg gacgaccgaa
    16321 aactaatccg ctttcgcgcg atgaacagct gcgtattaat aaacgcaacc agctaaaacg
    16381 cgacaaagta cgtggcctta agcgtgtcga actgaagctg aacgcggaag ctgtcgaggc
    16441 gctgaacgag ctggcggagt cgcgcaatat gagccgtagc gaactgatcg aagagatgtt
    16501 aatgcagcaa ctggcggcgc tgcgtagtca gggcatcgtt taaatttcca ctttcatgta
    16561 gcacagtgtg cagtcctgct cgtttgctga ttccgcaaga ctgcctgttc tgctatgatt
    16621 gcctttatcc gtgggcaatt ttccaccccc atttcaataa gtttcaagag gttatttcac
    16681 tcatggctat cactggcatc tttttcggca gcgacaccgg taataccgaa aatatcgcaa
    16741 aaatgattca aaaacagctt ggtaaagacg ttgccgatgt ccatgacatt gcaaaaagca
    16801 gcaaagaaga tctggaagct tatgacattc tgctgctggg catcccaacc tggtattatg
    16861 gcgaagcgca gtgtgactgg gatgacttct tcccgactct cgaagagatt gatttcaacg
    16921 gcaaactggt tgcgctgttt ggttgtggtg accaggaaga ttacgccgaa tatttctgcg
    16981 acgcattggg caccatccgc gacatcattg aaccgcgcgg tgcaacgatc gttggtcact
    17041 ggccaactgc gggctatcat ttcgaagcat caaaaggtct ggctgatgac gaccactttg
    17101 tcggtctggc tatcgacgaa gaccgtcagc cggaactgac cgctgaacgt gtagaaaaat
    17161 gggttaaaca gatttctgaa gagttgcatc tcgacgaaat tctcaatgcc tgatgtgatg
    17221 cggcgtagac tcatgtctac gccgtattaa tagataatgc caatcaaaat aattgctaca
    17281 aatttgtaac ttttgctgtt gtacctgtac aatgtcccgg tgttcaagtg gccttgccgt
    17341 tgtaaatgta agctgtgcca cgtttttatt aacaatattt gccagggact tgtggttttc
    17401 atttaggcgt ggcaattcta taatgatacg cattatctca agagcaaatt ctgtcacttc
    17461 ttctaatgaa gtgaaccgct tagtaacagg acagattccg catgactgat aacaataccg
    17521 ccctaaagaa agctggcctg aaagtaacgc ttcctcgttt aaaaatccta gaagttcttc
    17581 aggaaccgga caaccatcac gtcagtgcgg aagatttata caaacgtctg atcgatatgg
    17641 gtgaagaaat tggtctggct acggtatatc gcgtactgaa ccagtttgac gacgctggta
    17701 tcgtcacccg ccacaatttt gaaggcggta aatccgtatt tgaactgaca cagcaacatc
    17761 accacgatca cctgatctgc ctcgactgcg gcaaggttat cgaatttagt gatgattcca
    17821 tcgaagcgcg tcagcgtgaa attgccgcaa aacatggcat tcgcctgact aaccacagtc
    17881 tctatcttta cggtcactgt gccgaaggcg attgccgcga agatgaacat gcgcacgaag
    17941 gcaaataagc cagcctgaat gagaaaaagc caacctgcgg gttggctttt ttatgcaagg
    18001 gaaataagaa aagcgagagt tacagctctc acttatttgt tattactgcg aatttcctgc
    18061 caaactttat cacaatcggc tttcaccgcc tgatcatttc cggtttgcgt tgcacgcaga
    18121 tactgctgat aatccagtac gcggacactt tcctggtcgg caaaatgctg atgttgtttc
    18181 tctttcttca gcacgtttaa cacgctctgg caggcttcaa ttttttctgg cgaaccttgt
    18241 gcggtgttga tacaggcgct gtatgcctct ttcagacgac tgtcttcctg tggcgcggtt
    18301 gattgcgcac aagctaccag ccccgatgcc attatggcga tgagaatcag ttttttcata
    18361 gcaaattcct caaacgtgcg gcaggcacaa aacctgccgc gtcgggcatc agaagatggt
    18421 gaatggtgcg attaccataa atttcacgtc acgctcatcc tggaagatgt tgccgtaacc
    18481 accgccccag cttgggatgt cggagtggtt gtcgtattcg gtgaagtgca gtttgaacat
    18541 cgtgcctttg gcgcgaccgt cctgaatggt gtagaccgca tccaggctgt atgcagactc
    18601 ttcaatagtc cggtttttgt cgtagtacgc atccggattg ctctgctagg tcgcaggttt
    18661 agcatcccat gcgtaaacgt aggaagcgcc gatggcgaag cctggaagat tccagttttt
    18721 caggtcatac atcgcaccga agaagaccgc tttttcgccg ttagcgttga agtcggaacg
    18781 gttatcccac cagatatcca ggcggccgtt ggaggaagcg taggttggag tcatacgttg
    18841 caggaagtat ccctgctgac cgtcagcctt aacccaggtg ccttcgaggc gcaaatccac
    18901 tacgtcagcc gcccggtaac caaaggtcaa cgcttgcagc caggcggtgc cgtcataaag
    18961 gtcgttgacg ctgcgatcgt caactttatc gcgcgtacca tagaactggt agctggtggt
    19021 taacgggcta ccggcgatat caaatttgta gctggctttg gcaaaatatt gatcgatata
    19081 cccttccgcc tgaccaaatg ccgcttccag tacgaagtta tttttgaagt cgtatttcgc
    19141 cccaagggag tgcagataat caactttggt ggttttatcg ttctgataaa actcatccat
    19201 ttccagatgc cacggcgctt tgtattcgtt ggtccacatg taggagaaac tcaacgcacc
    19261 agcatcgccg taatcaaaat tcgccccggc ttccgccccc tgataagtac ctggcataaa
    19321 gctccagtgc ggcgctaaca gcgtttgacc agttggctga atgtaacctg ccctcgccca
    19381 aaccggaccg tatttaaatt tggccgcagc tttatacagg cttataccgc ttttgtcgcc
    19441 ggaccagtct tcgtcatagg ctttattact ttttgaaaac gcgatttcgt tcgggtggga
    19501 gctgtcgccg ttttccgcca tttcaatcgc cgtaaacgca gcaatatcaa ggccgaacat
    19561 atcagcagca taaccggact gaaaatcgag gttggcattc caggtggagt gagaaaggtt
    19621 ggttttgtat ttgtcgccgt cggtaacatc tttacggtcg cgttcacgct gccagtaata
    19681 tataccgccg gttaaggttg aatcgtcgat gaatccttcg gcgcgagcct ccggcattgc
    19741 cataaagccc gacattgctg taacaccggc gatagccagc gccagcgtac tacgtttgcc
    19801 actaaacgta cgcatgggtt aatcctcttt gacgtataaa ttgctgcacc aaaggtgaac
    19861 agcgaaaaaa taaaaattaa aaaagtagcg ttgagaaaag tctcgctgac tacaggaaca
    19921 gactattttt cgcgacgcga attatcaatc tctttctgta accagaatgt aagaacatga
    19981 gcaaccgcac atattttgat gatttttgtt acaaggctta agtttgcggt tgaaaggctt
    20041 gcagaagaat aaaagcgtga aattccagca agatgaaagc ggttacattt tatttaatta
    20101 tgttttgtta ttttaaattt attttatgga taaattgcga agcggaatta attcgcgaag
    20161 aaaagcagaa aaaacgccgc tgaacgcggc gttttggatt gtttgcatat ttcttgccgg
    20221 atgcggcgtg aacgccttaa ccggcctaca aaagcacgca aactcaatat attgcagaga
    20281 tcatgtaggc ctgataagcg tagtgcatca ggcaatttag cgtttgaaac tgtttgtctg
    20341 ttccttgccg gatgcgacgt aaacgcccca tccggcatag cgaaacttaa aattactcgc
    20401 ctactttcgc ccaggtatca cgcagcccaa cggtgcggtt aaataccggt ttttccgccg
    20461 tagaatggcg gctatcgagg cagaagtaac cttcacgctc aaactggaat gctttacccg
    20521 caaccgcatc tttcagcgac ggttcagcaa agccctgttt gatcaccagc gattccgggt
    20581 taatcaccga caggaaatca tccgcagcac cagggttcgg cacgctgaac agacggtcgt
    20641 acagacggat ttcaaccggc agcgcatgtg ccgcgctcac ccagtgaata acacctttga
    20701 ctttacgacc atctgccgga tctttgctta aggtgtcggc gtcataagta cagaagatgg
    20761 tggtgatatt gccttcggca tctttctcca cgcgttctgc cttaatgacg taagcattac
    20821 gcagacgcac ttctttaccc agcaccagac gtttgtactg cttgttagct tcttcgcgga
    20881 aatcggcgcg atcgatccaa atctcaccgc taaacggcac ctggcggcta cccatttccg
    20941 gtttgttcgg atggttcggc atggtaacca tttcgccttc gccctgatag ttttcgataa
    21001 ccagtttcac cggatcgata accgccattg cgcgcggggc gttttcgttg agatcttcac
    21061 ggatgcagga ttccagcgac gccatctcaa tggtgttgtc ctgcttggtc acgccgatgc
    21121 gtttgcagaa ctcacgaata gaagcagcag tgtaaccacg acgacgcaga ccggaaatgg
    21181 tcggcatacg cgggtcatcc cagccttcaa cgtgcttgtc ggtcaccagc aggttcagct
    21241 tacgcttgga catcacagtg tattccagat tcaggcgcga gaattcgtac tggcgcgggt
    21301 gaacaggaat ggtgatgttg tccagcaccc agtcgtacag gcgacggttg tcctggaact
    21361 caagcgtaca cagagagtgc gtaatacctt ccagggcatc gctgatgcag tgggtgaagt
    21421 cgtacatcgg gtagatgcac cacttgttgc cagtctggtg gtgttcagca aatttaatgc
    21481 ggtacagcac cggatcgcgc atcacgataa acggtgaagc catgtcgatt ttcgcacgca
    21541 ggcaggcttt accttcttca aaaccaccgg cacgcatttt ttcgaacagc gccaggttct
    21601 cttcaacgct gcggtcgcgg tacgggctgt ttttacccgg ttgcgtcagg gtgccgcggt
    21661 attcgcggat ctgttccggc gtcagttcat caacgtacgc caggccttta ttgatcagtt
    21721 cgatcgcata ggcgtggagc tgatcaaaat aatcggagga gtaacggacg ttaccagacc
    21781 agtgaaaacc taaccactct acgtcgtttt tgatcgactc aacatactcg atatcttctt
    21841 ttaccgggtt agtgtcgtcg aaacgcaggt tgcactggcc tttatagtcc tgggcgatcc
    21901 cgaagttcag gcagatagat ttcgcatggc caatatgcag atagccattc ggctccggcg
    21961 ggaaacgggt gtgtactgtg gtgtgcttac cactggccag atcttcatcg atgatctgac
    22021 ggataaagtt agtcgggcgg gcttctgcct cactcatcgt ggattcctca aagcgtaaac
    22081 aacgtataac ggcgtatgat cttataagcg ggacaggctg acaactgtta gttagcgaaa
    22141 atcgaaaatc tcactgagat tatggggatg attgatgaaa aaaaaagcgg cagagaagat
    22201 ctccaccgct tttgccagtc ccgcgtcatt gttggatgcg cgtcttatcc aaccaggact
    22261 gagcttttgt aggcctgata agacgcgtca agcgtcgcat caggcataaa gcagattact
    22321 ttttgatttc atacagcggt gtttgacccg ccacaacatt gccctgagct ttaatgatca
    22381 agccactgaa atcgtcgata ttgctgcaaa ccaccgggct aatcatcgaa cgggcgttag
    22441 cgttcaggta atccagatcc atttccagaa tcggttgccc tgcgctcacc tgcgcgccct
    22501 cttccaccag acgtttaaag cctttacctt ccagcgctac ggtgtcgata cccatgtgga
    22561 cgacaatctc cgcgcctttt tcggtttcta ggcagaacgc gtggttggtg ttgaagattt
    22621 tcacgattgt ccctgcggct ggtgatacga cgattttatc tgtcggtttc accgccacac
    22681 cgtcacccac cgctttgctg gcgaatgctt cgtcagaaac ctgatccagt gccacgacat
    22741 caccggtaat cggcgatacc agctccgcga tagataccgc gtttggtaca gcctgcggtt
    22801 ttgctacagg agcggcagtt gccggagttg cttcagctga cgcagctgct accgggccac
    22861 gggcaacgac tttcttcatc gcatcgccaa tggattctgc tttcgcgcca acaatcacct
    22921 gaatggtctg tttgttcagt tttactacac cagaagcacc cagacgttta cacatcgcat
    22981 cgttgacgcg agctgagtca gccacggtaa ggcgcagacg ggtaatacag gcgtcaatcg
    23041 ctttcaggtt gtcagtaccg ccaaccgcag caatatagtt ggttgccagt tgagtcagac
    23101 cttcttcagt attgctgtta gcctcttcag ttacgatctc gtcttcttta tcttcacgac
    23161 ccggcgtttt caggttgaac atgcggataa ccaaactgaa caccacgaag tagatagcga
    23221 agaagacaac acccatcacc agcagcatcc agacgttctg gctggcggcc ggcaggttat
    23281 acatcaacgc gtagtcgata gcccccgcag agaaagagaa gccagcatgg atacccagca
    23341 gcgttgccac aaacaggctg ataccggtca gcagtgcgtg caggaggtac agcagcggag
    23401 caaggaacat gaacaggaat tccagcggct cagtcacacc ggtcaggaac gcagtaacag
    23461 caacagaaag cagcatcccg ccaaccatcg gacgacgctc tttcggtgct gcgaagtaca
    23521 tcgccagcgc cgcacccggc agaccgaaca tcatgatcgg gaagaagccg gacatgaaca
    23581 tccccgcggt gccgtcacca gcgtagaaac ggttgatgtc gccgtggaaa accgtacccg
    23641 ccgcgttggt gaattcacca atctggaacc aggcgatggt gttcagcacc tgatgcagac
    23701 cggttgggat cagcagacgg ttgatgaaac caaagatacc ggaacccagc gcgcctgcag
    23761 aaacgatcca ctcgccgcct gcatggatag cgtgctgtac tggcggccag acgtaaccaa
    23821 aaatggccgc cagcaccagg cagaaaaagc cggtggcaat cggcacaaag cgtttgccgc
    23881 cgaagaagct caggaagtcc ggcagtttaa tatcggacca acggttatag gctgcgccac
    23941 caaccagacc ggtaatgata cccgccagta cacccatgtt aatttctggg ttgatggtca
    24001 ccatcgcttt ggttaacaca aagtaaccta ccgcacccgc cagcgccgcc gcacctgcgc
    24061 tgtctttcga ccagctggat gccacaccga tggcgaagat taatgcgagg ttatcaaaaa
    24121 tcgcaccgcc cgcctgggca ataaacgcaa cgttaagtaa atctggctga ccgaatcgca
    24181 gcaacagtgc cgccaccggc agcaccgcga tagggagctg taacgcccta ccgagtcgct
    24241 ggaaaaaacc taaaatattc atcttattcc ccctacgaga accctatttg gctcgtttaa
    24301 agccgtattt ttattttgct gcaaattgta ctgccgatgt tctgtaatca gattgttaga
    24361 tcatctgcta cagagtgtgt gaaaatttaa ttcgtatcgc aaattaaacg cgtgcctttt
    24421 gtgagttttg tcaccaaata tcgttatcat cactcccttt tactggctaa agcagaaaac
    24481 ttattttatc attcaaaaaa tcaggtcgga ttgacgcctg tctgcgcaaa tccaggttac
    24541 gcttaaagat gcctaatccg ccaacggctt acattttact tattgaggtg aataatgaga
    24601 ctgatccccc taactaccgc tgaacaggtc ggcaaatggg ctgctcgcca tatcgtcaat
    24661 cgtatcaatg cgttcaaacc gactgccgat cgtccgtttg ttctgggcct gccgactggc
    24721 ggcacgccga tgaccaccta taaagcgtta gtcgaaatgc ataaagcagg ccaggtcagc
    24781 tttaagcacg ttgtcacctt caacatggac gaatatgtcg gtctgccgaa agagcatccg
    24841 gaaagctact acagctttat gcaccgtaat ttcttcgatc acgttgatat tccagcagaa
    24901 aacatcaacc ttctcaacgg caacgccccg gatatcgacg ccgaatgccg ccagtatgaa
    24961 gaaaaaatcc gttcttacgg aaaaattcat ctgtttatgg gcggtgtagg taacgacggt
    25021 catattgcat ttaacgaacc ggcgtcttct ctggcttctc gtactcgtat caaaaccctg
    25081 actcatgaca ctcgcgtcgc gaactctcgt ttctttgata acgatgtcaa tcaggtgcca
    25141 aaatatgccc tgactgtcgg tgttggtaca ttgctggatg ccgaagaagt gatgattctg
    25201 gtgctgggta gccagaaagc actggcactg caggctgcag ttgaaggttg cgtgaaccac
    25261 atgtggacca tcagctgtct gcaactgcat ccaaaagcga tcatggtgtg cgatgaacct
    25321 tccaccatgg agctgaaagt taagacttta agatatttca atgaattaga agcagaaaat
    25381 atcaaaggtc tgtaattgtt atccctgccc tggctccttg ctcagggcaa tattttttaa
    25441 aatcgggggg cagaatgtat gcattaaccc agggccggat ctttaccggc cacgaatttc
    25501 ttgatgacca cgcggttgtt atcgctgatg gcctgattaa aagcgtctgt ccggtagcgg
    25561 aactgccgcc agagatcgaa caacgttcac tgaacggggc cattctctcc cccggtttta
    25621 tcgatgtgca gttaaacggc tgcggcggcg tacagtttaa cgacaccgct gaagcggtca
    25681 gcgtggaaac gctggaaatc atgcagaaag ccaatgagaa atcaggctgt actaactatc
    25741 tgccgacgct tatcaccacc agcgatgagc tgatgaaaca gggcgtgcgc gttatgcgcg
    25801 agtacctggc aagacatccg aatcaggcgt taggtctgca tctggaaggt ccgtggctga
    25861 atctggtaaa aaaaggcacc cataatccga attttgtgcg taagcctgat gccgcgctgg
    25921 tcgatttcct gtgtgaaaac gccgacgtca ttaccaaagt gaccctggca ccggaaatgg
    25981 ttcctgcgga agtcatcagc aaactggcaa atgccgggat tgtggtttct gccggtcact
    26041 ccaacgcgac gttgaaagaa gcgaaagccg gtttccgcgc ggggattacc tttgccaccc
    26101 atctgtacaa cgcgatgccg tatattaccg gtcgtgaacc gggcctggcg ggcgcgatcc
    26161 tcgacgaagc tgacatttat tgcggtatta tcgctgatgg cctgcatgtt gattacgcca
    26221 acattcgtaa cgctaaacgc cttaaaggcg acaaactgtg tctggttacc gacgccaccg
    26281 cgccagcagg tgccaacatt gaacagttca tttttgcggg taaaacaata tactaccgta
    26341 acggactttg tgtggatgag aacggtacgt taagcggttc atccttaacc atgattgaag
    26401 gcgtgcgtaa tctggtcgaa cattgcggta tcgcactgga tgaagtgctg cgtatggcga
    26461 cgctctatcc ggcgcgtgcg attggcgttg agaaacgtct cggcacactc gccgcaggta
    26521 aagtagccaa cctgacctca ttcacacctg attttaaaat caccaagacc atcgttaacg
    26581 gtaacgaggt cgtaactcaa taagagaaag tatgacacca ggcggacaag ctcagatagg
    26641 taatgttgat ctcgtaaaac agcttaacag cgcggcggtt tatcgcctga ttgaccagta
    26701 cgggccaatc tcgcggattc agattgccga gcaaagccag cttgcccccg ccagcgtaac
    26761 caaaattacg cgtcagctta tcgaacgcgg gctgatcaaa gaagttgatc agcaggcctc
    26821 caccgggggc cgccgcgcta tctccatcgt caccgaaacc cgcaatttcc acgcaatcgg
    26881 cgtacggctt ggtcgtcacg acgccaccat caccctgttt gatctcagca gcaaagtgct
    26941 ggcagaagaa cattacccgc tgccggaacg tacccagcaa acgctggaac atgccctgtt
    27001 gaatgccatt gctcagttta ttgatagcta ccagcgcaaa ctgcgcgagc tgatcgcgat
    27061 ttcggtgatc ctgccagggc ttgttgaccc ggacagcggc aaaattcatt acatgccgca
    27121 tattcaggta gaaaactggg ggctggtaga agctctggaa gaacgtttta aagtgacctg
    27181 tttcgttggt cacgatatcc gtagtctggc gctggcggag cactacttcg gtgcaagtca
    27241 ggattgcgaa gactccattc tggtgcgtgt ccatcgcgga accggggccg ggattatctc
    27301 taacgggcgc atttttattg gccgcaacgg taacgtcggt gaaattggcc atattcaggt
    27361 cgaaccgctg ggtgaacgct gccactgcgg caactttggc tgcctggaaa ctatcgccgc
    27421 caacgctgcc attgaacaac gggtgttgaa tctgttaaag cagggctacc agagccgcgt
    27481 gccgctggac gactgcacca tcaaaactat ctgcaaagcc gcgaacaaag gcgatagtct
    27541 ggcgtcggaa gtaattgagt atgtcggtcg tcatctgggt aaaaccatcg ccattgctat
    27601 caacttattt aatccgcaaa aaattgttat tgccggtgaa atcaccgaag ccgataaagt
    27661 gctgctccct gctattgaaa gctgcattaa tacccaggcg ctgaaggcgt ttcgtactaa
    27721 tctgccggtg gtacgttctg agctggatca ccgctcggca atcggcgctt ttgcgctggt
    27781 aaaacgcgcc atgctcaacg gtattttgct ccagcatttg ctggaaaatt aatgtgcttt
    27841 tatagtggcg cttattgttg tcaatattct gggtagtcca tgaccattaa aaatgtaatt
    27901 tgcgatatcg acggcgtgct gatgcacgat aacgtcgccg taccgggtgc agcggaattt
    27961 ttgcacggga ttatggataa aggcctgccg ctggtgttgc tgaccaacta tccttcgcag
    28021 accgggcaag atctggcgaa ccgctttgcc actgccggtg tcgatgtgcc ggatagcgtg
    28081 ttttatacct ctgcgatggc gaccgccgat ttcctgcgtc gccaggaagg caagaaagca
    28141 tatgtggtgg gcgaaggcgc actgattcat gaactgtaca aagccggttt cactattacc
    28201 gatgtgaacc ctgatttcgt gattgttggc gaaacgcgtt cctacaactg ggacatgatg
    28261 cataaagcgg cctatttcgt cgctaacggt gcacgcttta tcgccaccaa cccggacacc
    28321 cacgggcgcg gtttttatcc cgcttgtggc gcgttgtgtg cagggattga gaaaatctcc
    28381 gggcgcaaac cgttctatgt tggtaagccc agcccgtgga tcatccgcgc agcattaaac
    28441 aaaatgcagg cgcattcgga agaaacggtg attgtcggcg ataacttgcg caccgacatt
    28501 ctggcgggct tccaggcagg tctggaaacg attctggtgc tttctggtgt ttcatcgctc
    28561 gacgatatcg acagtatgcc gttccgccca agctggattt acccgtcagt cgctgaaatc
    28621 gacgttatct gaataccacc cacgtctgcg gacgtggttg gctctgatga caaacgccaa
    28681 actgcctgat gcgctacgct tatcaggcct acgcagctcc tgcaatatat tgaattttca
    28741 tgcttttgta ggccggataa ggcgttcacg ccgcatccgg catgaacaaa tcgcacttta
    28801 tcagcaattt ggcc
//
LOCUS       DADMKF010000040        28702 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.041, whole genome shotgun sequence.
ACCESSION   DADMKF010000040 DADMKF010000000
VERSION     DADMKF010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28702
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.041"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(36..119)
                     /locus_tag="JNC24_001591"
                     /pseudo
     CDS             complement(36..119)
                     /locus_tag="JNC24_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YebV family protein"
     gene            complement(237..1676)
                     /gene="rsmF"
                     /locus_tag="JNC24_001592"
     CDS             complement(237..1676)
                     /gene="rsmF"
                     /locus_tag="JNC24_001592"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HAY9158359.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKISVADFLQLTAPYGWTLTPIPWCEEGFWIERDNEDALPLGSTAEHLSGLFYIQEA
                     SSMLPVAALFADGNAPQRVMDVAAAPGSKTTQISARMNNEGAILANEFSASRVKVLHA
                     NISRCGISNVALTHFDGRVFGAAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNQEENEAVCLWLKETYPDAVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPAPKYKVGNFPFSPVKD
                     REAGQIRQAAAGVGLNWDENLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIALATPDNVNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTGNA"
     gene            complement(1757..4390)
                     /gene="yebT"
                     /locus_tag="JNC24_001593"
     CDS             complement(1757..4390)
                     /gene="yebT"
                     /locus_tag="JNC24_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
                     /protein_id="HAY9158360.1"
                     /translation="MSQETPASTTEAQIKNKRRISPFWLLPFIALMIAGWLIWDSYQD
                     RGNTVTIDFMSADGIVPGRTPVRYQGVEVGTVQDISLSDDLRKIEVKVSIKSDMKDAL
                     REETQFWLVTPKASLAGVSGLDALVGGNYIGMMPGKGKEQDHFVALDTQPKYRLDNGD
                     LMIHLQAPDLGSLNSGSLVYFRKIPVGKVYDYAINPNKQGVVIDVLIERRFTDLVKKG
                     SRFWNVSGVDANVSISGAKVKLESLAALVNGAIAFDSPEESKPAEAEDTFGLYEDLAH
                     SQRGVIIKLELPSGAGLTADSTPLMYQGLEVGQLTKLDLNPGGKVTGEMTVNPSVVTL
                     LRENTRIELLNPKLSLSDANLSALLTGKTFELVPGDGEPRKEFVVVPGEKALLHEPDV
                     LTLTLTAPESYGIDAGQPLILHGVQVGQVIDRKLTSKGVTFTVAIEPQHRELVKGDSK
                     FVVNSRVDVKVGLDGVEFLGASASEWINGGIRILPGDKGEMKASYPLYANLEKALENS
                     LSDLPTTTVSLSAETLPDVQAGSVVLYRKFEVGEVITVRPRANAFDIDLHIKPEYRNL
                     LTSNSVFWAEGGAKVQLNGSGLTVQASPLSRALKGAISFDNLSGASASQRKGDKRILY
                     ASETAARAVGGQITLHAFDAGKLAVGMPIRYLGIDIGQIQTLDLITARNEVQAKAVLY
                     PEYVQTFARGGTRFSVVTPQISAAGVEHLDTILQPYINVEPGRGNPRRDFELQEATIT
                     DSRYLDGLSIIVEAPEAGSLGIGTPVLFRGLEVGTVTGMTLGTLSDRVMIAMRISKRY
                     QHLVRNNSVFWLASGYSLDFGLTGGVVKTGTFNQFIRGGIAFATPPGTPLAPKAQEGK
                     HFLLQESEPKEWREWGTALPK"
     gene            complement(4359..5642)
                     /gene="yebS"
                     /locus_tag="JNC24_001594"
     CDS             complement(4359..5642)
                     /gene="yebS"
                     /locus_tag="JNC24_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HAY9158361.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDTITGAMVFFCVIGAPLILVSSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERYY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGRCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            5772..6269
                     /locus_tag="JNC24_001595"
     CDS             5772..6269
                     /locus_tag="JNC24_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HAY9158362.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFGRFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            6366..7064
                     /gene="proQ"
                     /locus_tag="JNC24_001596"
     CDS             6366..7064
                     /gene="proQ"
                     /locus_tag="JNC24_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HAY9158363.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDALRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            7084..9132
                     /gene="prc"
                     /locus_tag="JNC24_001597"
     CDS             7084..9132
                     /gene="prc"
                     /locus_tag="JNC24_001597"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HAY9158364.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNALWDSKVKFDELSLKLAGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FGPELLKEHNARIAKDPEFQNIMKDIARFNAMRDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            9324..10205
                     /gene="htpX"
                     /locus_tag="JNC24_001598"
     CDS             9324..10205
                     /gene="htpX"
                     /locus_tag="JNC24_001598"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HAY9158365.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(10251..11314)
                     /locus_tag="JNC24_001599"
                     /pseudo
     CDS             complement(10251..11314)
                     /locus_tag="JNC24_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            11491..12282
                     /gene="kdgR"
                     /locus_tag="JNC24_001600"
     CDS             11491..12282
                     /gene="kdgR"
                     /locus_tag="JNC24_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HAY9158366.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDLDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            complement(12425..12664)
                     /locus_tag="JNC24_001601"
     CDS             complement(12425..12664)
                     /locus_tag="JNC24_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9158367.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLGITDCPLLRKSNIVVDNG"
     gene            12823..12966
                     /gene="mgrB"
                     /locus_tag="JNC24_001602"
     CDS             12823..12966
                     /gene="mgrB"
                     /locus_tag="JNC24_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HAY9158368.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            complement(13134..14114)
                     /locus_tag="JNC24_001603"
     CDS             complement(13134..14114)
                     /locus_tag="JNC24_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000019445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5-like element ISKpn26 family transposase"
                     /protein_id="HAY9158369.1"
                     /translation="MSHQLTFADSEFSTKRRQTRKEIFLSRMEQILPWQNMTAVIEPF
                     YPKAGNGRRPYPLETMLRIHCMQHWYNLSDGAMEDALYEIASMRLFARLSLDSALPDR
                     TTIMNFRHLLEQHQLARQLFKTINRWLAEAGVMMTQGTLVDATIIEAPSSTKNKEQQR
                     DPEMHQTKKGNQWHFGMKAHIGVDAKSGLTHSLVTTAANEHDLNQLGNLLHGEEQFVS
                     ADAGYQGAPQREELAEVDVDWLIAERPGKVKTLKQHPRKNKTVINIEYMKASIRARVE
                     HPFRIIKRQFGFVKARYKGLLKNDNQLAMLFTLANLFRVDQMIRQWERSQ"
     gene            14241..14528
                     /locus_tag="JNC24_001604"
     CDS             14241..14528
                     /locus_tag="JNC24_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9158370.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTIESQKSVDDEDIIRLVAER"
     gene            15198..15341
                     /locus_tag="JNC24_001605"
     CDS             15198..15341
                     /locus_tag="JNC24_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobF family protein"
                     /protein_id="HAY9158371.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            15354..15563
                     /gene="cspE"
                     /locus_tag="JNC24_001606"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             15354..15563
                     /gene="cspE"
                     /locus_tag="JNC24_001606"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HAY9158372.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            15729..16538
                     /gene="rlmA"
                     /locus_tag="JNC24_001607"
     CDS             15729..16538
                     /gene="rlmA"
                     /locus_tag="JNC24_001607"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HAY9158373.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLSVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLDDKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTFCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSAELCY
                     PMRLRGDEAVALLQMTPFTWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(16535..17101)
                     /gene="mntP"
                     /locus_tag="JNC24_001608"
     CDS             complement(16535..17101)
                     /gene="mntP"
                     /locus_tag="JNC24_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HAY9158374.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGATLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
     gene            complement(17530..17988)
                     /locus_tag="JNC24_001609"
     CDS             complement(17530..17988)
                     /locus_tag="JNC24_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 domain-containing protein"
                     /protein_id="HAY9158375.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRVPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLLVSTQ"
     gene            complement(18043..18894)
                     /locus_tag="JNC24_001610"
     CDS             complement(18043..18894)
                     /locus_tag="JNC24_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HAY9158376.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVSR
                     ITDQTGKEHVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            complement(18907..19707)
                     /locus_tag="JNC24_001611"
     CDS             complement(18907..19707)
                     /locus_tag="JNC24_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HAY9158377.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            complement(19770..20741)
                     /gene="manX"
                     /locus_tag="JNC24_001612"
     CDS             complement(19770..20741)
                     /gene="manX"
                     /locus_tag="JNC24_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HAY9158378.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            21204..22754
                     /gene="yoaE"
                     /locus_tag="JNC24_001613"
     CDS             21204..22754
                     /gene="yoaE"
                     /locus_tag="JNC24_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HAY9158379.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPIPE
                     GAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLFPV
                     CRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRARGS
                     FVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGDGWLVKGGTDLHALQQA
                     LDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIIEANDYRVDLVRIVKE
                     QPAHDEDE"
     gene            complement(22758..24356)
                     /locus_tag="JNC24_001614"
     CDS             complement(22758..24356)
                     /locus_tag="JNC24_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HAY9158380.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVMEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQRFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTR
                     YVMAETIRQRHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDMLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMPLR"
     gene            complement(24487..25851)
                     /locus_tag="JNC24_001615"
     CDS             complement(24487..25851)
                     /locus_tag="JNC24_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine dehydratase 1"
                     /protein_id="HAY9158381.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVEERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            complement(26035..26613)
                     /locus_tag="JNC24_001616"
     CDS             complement(26035..26613)
                     /locus_tag="JNC24_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HAY9158382.1"
                     /translation="MEYRSLMLDDFLSRFQLLRPQINRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVKP"
     gene            complement(26617..27978)
                     /gene="pabB"
                     /locus_tag="JNC24_001617"
     CDS             complement(26617..27978)
                     /gene="pabB"
                     /locus_tag="JNC24_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HAY9158383.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTLGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTREQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITAQLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            28052..28231
                     /locus_tag="JNC24_001618"
     CDS             28052..28231
                     /locus_tag="JNC24_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HAY9158384.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGERIVARFEDEDE"
     gene            complement(28351..28674)
                     /locus_tag="JNC24_001619"
     CDS             complement(28351..28674)
                     /locus_tag="JNC24_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001442732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HAY9158385.1"
                     /translation="MVNFKDKNMPAVIDKALDFIGAMDVSAPTPSSMNESTAKGIFKY
                     LKELGVPASAADITARADQEGWNPGFTEKMVGWAKKMESGERSVIKNPEYFSTYMQEE
                     LKALV"
BASE COUNT         7311 a         7087 c         7275 g         7029 t
ORIGIN      
        1 cataaaacac tatcaataag ttggagtcat tacctggtta acgttcgatc acccggcgat
       61 tcaccgtgta attcgccgtc gtcgatttca aaagcgccta tgcgcacact ggttttcatc
      121 ttcgtgctct ccatttagct gccggttgtg gtacgaccag tccgatcgca ccatttttca
      181 gcctagttga ggcagccgaa tgtgccagtc aaaaagtgcg ctttttttgt gcgctgtcag
      241 gcgttaccgg taaaaagttt cccatcgcgc accagttcac gcggatagct gtttttcaat
      301 cgcgaaccaa tccgtttggc taaaccaatc ggttgatgct ggaaagtaac caacacgtca
      361 tccgccactg gcgcggcttg cgggtaaaca tcgcgcccgc gataccactc ctccgcttcc
      421 tgcggtgtca gttcaaaagc gttcacattg tcgggggtgg caagggcaat aactgcttca
      481 tgctgccagc gataaccttt gttgtgcgtt tcggcaagtt taatccccaa gcgagaaaat
      541 cggactttac cgatcagggc ttcaatgccc accgggaaca accacagttc tttgtcacgc
      601 tgccagaggc gcaggttttc atcccagttt aagccaacac ctgcagccgc ctgacgaatt
      661 tgtccagctt ctcgatcttt cactgggctg aacggaaaat taccgacttt gtatttgggg
      721 gcgggtaagg cgggaatcgc ctgagtttta cgcagacgag caacgaagaa gccttcgcag
      781 tcgtaaattt gtgggaaaac atgcaaaaag ccttcctcgg tcagcgcctt gtttgcacca
      841 gggaagagat caccaagcgg taaaaactct actgcgtcgg ggtaagtctc tttcagccac
      901 aggcaaacgg cttcgttttc ttcctggttt aaggtacagg tcgagtaaac cagcgtaccg
      961 ccagggcgta atgcatgaaa ggcgctgtcg atcagttccc gttgggtagc tgcgatttcc
     1021 tgattgcttt ctggtgacca gttttttagc gcatcgggat ctttacgcac cacgccttcg
     1081 ccggagcagg gagcgtccag caaaatggca tcaaacattt ctggcactgc cgcaccaaac
     1141 acgcggccat caaaatgtgt gagcgcaaca ttactgatac cacagcggct gatattggca
     1201 tgtaacactt ttacccgact ggcggaaaac tcattggcaa gaattgcccc ttcgttattc
     1261 atccgcgcgg aaatttgcgt cgttttggag ccaggcgcgg cagcgacatc catcacccgc
     1321 tgtggtgcat taccgtcagc aaacaaggcg gcgacgggca acattgaact ggcttcctga
     1381 atataaaaca ggccacttaa atgctcggcg gtactaccca atggcaatgc atcttcattg
     1441 tcgcgttcaa tccagaaacc ttcttcacac cacggaattg gcgtaagcgt ccagccataa
     1501 ggagcggtta attgcaggaa atcagcaacg gagattttca gcgtattaac gcgaatgctg
     1561 cggcgcaacg ggcgctgaca ggcggcaaga aaatcatcaa aagagagcgt cgaaggcatc
     1621 gcttcgcgca tttgcgtcag aaaggcgtcc gggaaataaa cggtgtgttg ggccacgagc
     1681 atataccact ggcaaaaaaa acaggcgcgc agtttagcat aaacgctccg gcgcaggcac
     1741 gccggagcag tgggcattat ttgggaagcg cagtacccca ttcacgccac tctttcggtt
     1801 cactttcctg caacaggaag tgcttgcctt cctgggcttt cggtgccagt ggcgtacccg
     1861 gaggcgtggc gaaggcgatg ccgccacgga taaactggtt aaaggtgccg gttttcacta
     1921 cgccgcccgt cagaccaaag tccagactgt aacccgatgc caaccagaag acggaattgt
     1981 tacgcaccag gtgttgatag cgtttactga tgcgcatcgc aatcatcacg cgatctgaca
     2041 atgtccccag cgtcattccg gtaaccgtac ctacttccag accacggaac agcacaggcg
     2101 taccaatacc taacgaaccg gcttccggtg cttcgacaat aatgcttaag ccatccaggt
     2161 aacgcgaatc agtaatagta gcttcttgca actcaaagtc acggcgagga ttaccccgac
     2221 ctggttcgac attgatatac ggctggagga tagtatctag atgctcaacg cccgcggctg
     2281 aaatttgcgg tgtgaccacc gagaagcgcg taccgccgcg ggcgaaggtc tggacatact
     2341 ccggatagag caccgccttt gcctgtactt cattgcgcgc ggtaatcaga tccagcgtct
     2401 ggatttgccc gatatcaata ccgagatagc gaattggcat cccgaccgcc agttttccgg
     2461 catcgaaagc gtgaagcgta atctgcccac caaccgcacg ggccgctgtt tcggaagcat
     2521 acagaattcg tttgtcgcct ttacgctgac tggcgctggc accgctgagg ttatcgaagc
     2581 taatggctcc ctttaatgct ctggagagcg gggatgcctg tacggtcagg ccactaccat
     2641 tcagctgaac tttcgccccg ccttctgccc agaacacgct attgctggtc agaaggttgc
     2701 gatactccgg cttaatatgc agatcgatat caaacgcgtt agctcgcgga cgcacggtaa
     2761 tcacttcacc aacttcaaat ttacggtaca gcactaccga tcctgcctgc acatccggca
     2821 gcgtctctgc actcaaactc acggttgtgg tgggtaaatc gctaaggctg ttctccagcg
     2881 ctttttccag attggcatac agtggatagc tggctttcat ctcaccttta tcgcctggca
     2941 gaatacgtat cccgccgtta atccattctg aggcgctggc accaagaaac tcaacgccat
     3001 ccagccccac cttcacgtcg acacggctgt tgacgacaaa tttgctatcg ccttttacca
     3061 gttcgcgatg ctgaggctcg atggcgacgg taaaggtaac gcctttgctg gtgagtttac
     3121 gatcaataac ctggcctacc tgcacgccgt gaagaatgag cggctgacca gcatcaatac
     3181 cgtaactttc cggcgcggtc agggtcagcg tcagaacatc aggttcatgc agcagtgctt
     3241 tttcgcctgg cacaacaacg aactctttgc gtggctcgcc atcgccgggt accaactcga
     3301 aggttttgcc agtcagcagg gcgctgagat tggcatcgct aagggataat ttcggattaa
     3361 gtaattcgat gcgggtattt tcacgaagca gggtaacgac gctgggatta actgtcattt
     3421 ccccagtgac tttaccacca ggatttaaat ccagtttagt tagctgtccg acttccagtc
     3481 cctgatacat taatggcgtc gagtcggcgg ttaatccggc cccactcggc agttccagtt
     3541 tgattattac gccacgctgg ctatgggcga ggtcttcata cagaccaaag gtatcttccg
     3601 cctcggcagg tttcgactct tcaggtgaat cgaaggcaat cgcaccgtta accagtgccg
     3661 ccagactttc cagtttcacc ttcgcaccac tgatactgac gttggcatca acgccggaaa
     3721 cgttccagaa acggctacct tttttcacta ggtcggtaaa acgccgctcg atcaggacat
     3781 caatcaccac gccttgcttg ttgggattga tggcatagtc gtagactttt cccacaggga
     3841 tcttgcggaa atagaccaat gaaccgctgt tcagcgaacc gagatcgggg gcttgcaggt
     3901 ggatcatcag atcgccattg tccagtcgat atttcggttg ggtatcgagt gcgacaaagt
     3961 gatcctgctc tttaccttta cctggcatca tgccgatata gttaccaccg acgagggcgt
     4021 ccagcccgga gacacctgcc aacgatgctt ttggcgtcac cagccagaac tgagtctctt
     4081 cgcgcagcgc atctttcata tcggacttga tgctgacctt gacttcaatc ttacgaagat
     4141 cgtcgctgag gctgatatcc tgcactgttc cgacttcaac gccctgataa cgaacaggcg
     4201 tacggcccgg gacaatacca tccgccgaca taaagtcgat ggtgacggta ttaccccggt
     4261 cctgataact gtcccaaatc agccaaccgg caatcattag cgcgatgaaa ggcagcagcc
     4321 agaaaggtga gatacggcgt ttatttttaa tctgcgcttc agtcgtcgaa gcgggcgttt
     4381 cctgactcat gtgcatccca aagtaagcgg ctgtccagcc attccacagc aagaatagtc
     4441 aaaattacgg ctgcgccaaa ataaaacgca gccggtccca tagtaaaagc gaggatctga
     4501 tcgcgattaa tcagcgacat ggttaaagat atgacaaaca ggtcgagcat cgaccagcgt
     4561 ccaatccagg tcaccatccg cagtaacaga atgcgtgtgc gtaaaccttg ctggcattta
     4621 aaatgaatgc tcaacagtaa agtgaacatg acgatcactt tagtaaacgg taccagaata
     4681 ctggcgataa acacgattcc ggcgaccgca atgttgctac ttgccagcga cataatcccg
     4741 gaaagaatcg tatcttcctg ccggccacca ttcagataaa tgatagaaat aggcaatagg
     4801 ttggctggta acaacaaaac gattgacgct aacagcgccg cccagcattt ttgcaaacta
     4861 tgacgacgac gcaggcgtag cgggatatgg caacgcgggc agcgaccacg ctgatcagga
     4921 tagccggtaa aatggcaccc aagacagaca cgaagtttct cgtccctacg cgtagcgggg
     4981 cgctgcggat aatatcgctc ccacagctcc tcaacattaa gatgtgacaa cgtcaccgtc
     5041 gttaaaatca ccaacgccac aaaagagaac aagccgacac ccgcctggat atgggcataa
     5101 tcctgtacct ttatagaagc aacgccaatg ccgaccaggt agatatccag cattacccac
     5161 tctttaagac gctcaagcat cagcagtacc ggacgtaaat tcattcccag tcggttacca
     5221 aaccataaat atgctatgga ggacaccaga atgagggggg caccaataac gcagaaaaag
     5281 accatcgccc ccgttatcgt atcgccctgt ttggtcattt gccagatgcc ttgcatgacg
     5341 ttggcgtcga tacgaatacc taacagccag atatgcaaca gcggttcgcc ccaggcaaac
     5401 ggcatcagca acagcatggt gaaagccatt gccgccaggc gcgttagcga ccagtcgcgc
     5461 ccgtcgcgaa tttttgcctg acagcgcgga caataggcac tctgatgaga atttatctcg
     5521 ggcaggctaa acagcatgtc acattgcggg caacgttggt aatcaccacg cggcagttcc
     5581 tcgccgattg ccctcactgt tatctttttt gtcggcgtaa tttgtggtgt gttaagagcc
     5641 atgacgtgcc aaatgtaata agaattaaca ttaatcttaa cccatgatgg gaatgatctt
     5701 gagcattaac gcatttaatg cttattttaa taggctggca gttccaggta agacaaacaa
     5761 gtgattatat aatgaacaaa acagaatttt acgcggattt aaatcgcgac tttaacgcgc
     5821 tgatggcggg agaaaccagt tttctggcaa cgcttgcgaa caccagtgcg ttgttatacg
     5881 agcgtctcac tgacgtaaac tgggcaggtt tttatttgct tgaggacgat acactggtac
     5941 tcggaccatt tcagggcaaa attgcctgtg tccggatacc cgtcgggcgc ggggtgtgcg
     6001 gcactgcggt tgcccgcaat caagtgcagc gtatcgagga tgttcacgcg tttgacgggc
     6061 atattgcctg tgatgcggcg agtaattctg aaattgttct gccgctggtg gtgaaaaatc
     6121 agattattgg tgttcttgac atcgatagta ccgtcttcgg tcgctttaca gacgaggacg
     6181 agcaaggctt acgtcagctt gtggcacagc ttgaaaaagt gcttgcaacg acggattaca
     6241 aaaaattctt tgcgagcgtc gcaggataat caacggataa cgtagcattt actggtagcg
     6301 tcattataat gacgcctgtt catgcctgcg cttgttggct acgtccgttg taatcaggaa
     6361 atttcatgga aaatcaacct aagttgaata gcagtaaaga agtaatcgcg tttctggccg
     6421 aacgttttcc ccactgtttc agtgcggaag gtgaagcgcg tccgctgaaa atcggtattt
     6481 ttcaggattt ggtcgatcgt gttgccgggg aaatgaacct gagcaaaacg caattacgat
     6541 ccgctttacg tctctacact tcgagctggc gttatcttta cggtgttaaa cccggcgcaa
     6601 cgcgtgtcga tcttgacggc aacccatgcg gtgagctgga cgagcaacat gtagagcatg
     6661 ctcgcaagca gcttgaagaa gcgaaagcgc gtgttcaggc acagcgtgct gaacagcaag
     6721 cgaaaaaacg cgaagctgcc gcagctgctg gtgagaaaga agacgcactg cgccgcgaac
     6781 gcaagccacg tccgaccacg ccacgccgca aagaaggcgc tgaacgtaaa cctcgtgcgc
     6841 aaaagccggt agagaaagcg ccaaaaacag taaaagcacc tcgcgaagaa cagcacaccc
     6901 cggtttctga catttcagct ctgactgtcg gacaagccct gaaggtgaaa gcgggtcaaa
     6961 acgcgatgga tgccaccgta ttagaaatca ccaaagatgg cgtccgcgtc cagctgaatt
     7021 cgggtatgtc tttgattgtg cgcgcagaac acctggtgtt ctgaaacgga ggccgggcca
     7081 ggcatgaaca tgttttttag gcttaccgcg ttagctggcc tgcttgcaat agcaggccag
     7141 accttcgctg tagaagatat cacgcgtgct gatcaaattc cggtattaaa ggaagagacg
     7201 cagcatgcga cggtaagtga gcgcgtaacg tcgcgcttca cccgttctca ttatcgccag
     7261 ttcgacctcg atcaggcatt ttcggccaaa atctttgacc gctacctgaa tctgctcgat
     7321 tacagccaca acgtgctgct ggcaagcgat gttgaacagt tcgcgaaaaa gaaaaccgag
     7381 ttaggcgatg aactgcgttc aggcaaactc gacgttttct acgatctcta caatctggcg
     7441 caaaagcgcc gttttgagcg ttaccagtac gctttgtcgg tactggaaaa gccgatggat
     7501 ttcaccggca acgacactta taaccttgac cgcagcaaag cgccctggcc gaaaaacgag
     7561 gctgagttga acgcgctgtg ggacagtaaa gtcaaattcg acgagttaag cctgaagctg
     7621 gcaggaaaaa cggataaaga aattcgtgaa accctgacac gccgctacaa atttgccatt
     7681 cgtcgtctgg cgcaaaccaa cagcgaagat gttttctcgc tggcaatgac ggcgtttgcg
     7741 cgtgaaatcg acccgcatac caactatctt tccccgcgta ataccgaaca gttcaacact
     7801 gaaatgagtt tgtcgctgga aggtattggc gcagtgctgc aaatggatga tgactacacc
     7861 gttatcaatt cgatggtggc aggtggtccg gcagcgaaga gtaaagctat cagcgttggt
     7921 gacaaaattg tcggtgttgg tcaaacaggc aagccgatgg ttgacgtgat tggctggcgt
     7981 cttgatgatg tggttgcctt aattaaaggg ccgaagggca gtaaagttcg tctggaaatt
     8041 ttacctgctg gtaaagggac caagacccgc actgtaacat tgacccgtga acgtattcgt
     8101 ctcgaagacc gcgcggttaa aatgtcggtg aagaccgtcg gtaaagagaa agtcggtgtg
     8161 ctggatattc cgggcttcta tgtgggtttg acagacgatg tcaaagttca actgcagaaa
     8221 ctggaaaaac agaatgtcag cagtgtgatc atcgacctgc gtagcaatgg cggtggggcg
     8281 ttgaccgaag cggtatcgct ctcaggtctg tttattcctg cgggtcccat tgttcaggtc
     8341 cgcgataaca acggcaaggt tcgtgaagac agcgataccg acgggcaggt gttctataaa
     8401 ggcccgctgg tggtactggt tgaccgtttc agtgcttcgg cttcagaaat ctttgccgcg
     8461 gcaatgcagg attacggtcg tgcgctggtt gtgggtgaac cgacgtttgg taaaggcacc
     8521 gttcagcaat accgttcatt gaaccgtatt tacgatcaga tgttacgtcc tgaatggcca
     8581 gcgctgggtt ctgtgcagta cacgatccag aaattctatc gcgttaacgg cggcagtacg
     8641 caacgtaaag gcgtaacgcc agacatcatc atgccgacgg gtaatgaaga aacggaaacg
     8701 ggtgagaaat tcgaagataa cgcgctgccg tgggatagca ttgatgccgc gacttatgtg
     8761 aaatcaggcg atttaacggc ctttgggccg gagctgctga aggaacataa tgcgcgtatc
     8821 gcgaaagatc ctgagttcca gaacatcatg aaggatatcg cgcgcttcaa cgctatgagg
     8881 gacaagcgca atatcgtttc tctgaactac gctgtgcgtg agaaagagaa taatgaagat
     8941 gatgcgacgc gtctggcgcg tttgaacgaa cgctttaaac gcgaaggtaa accggagttg
     9001 aagaagctgg atgatctacc gaaagattac caggagccgg atccttatct ggatgagacg
     9061 gtgaatatcg cactcgatct ggcgaagctt gaaaaagcca gacccgcgga acaacccgct
     9121 cccgtcaagt aatatcaatc aggcacaaga aattgtgcct gattttttaa cagcggcaag
     9181 atgccgtaaa tcagatgcta caaaatgtaa agttgtgtct ttctggtgac ttacgcacta
     9241 tccagacttg aaaatagtcg cgtaacccat acgatgtggg tatcgcatat tgcgttttgt
     9301 taaactgagg taaaaagaaa attatgatgc gaatcgcgct cttcctgcta acgaacctgg
     9361 ccgtaatggt cgttttcggg ctggtactga gcctgacagg gatacagtcg agcagcgttc
     9421 aggggctgat gatcatggcc ttgctgttcg gttttggtgg ttccttcgtt tcgcttctga
     9481 tgtccaaatg gatggcatta cgatctgttg gcggggaagt gatcgagcaa ccgcgtaacg
     9541 aaagggaacg ttggctggtc aatactgtag caacccaggc tcgtcaggca gggatcgcta
     9601 tgccgcaagt ggctatctac catgcgccgg acatcaacgc ttttgcaacc ggtgcgcgcc
     9661 gtgatgcctc tctggttgct gtcagcaccg gtttgctgca gaacatgagc ccggatgaag
     9721 ccgaggcggt aattgctcac gaaatcagcc acatcgccaa tggtgatatg gtcaccatga
     9781 cgctgattca gggcgtggtg aacaccttcg ttatctttat ttcccgtatt ctggcgcagc
     9841 ttgccgcggg ttttatgggc ggaaatcgtg atgaaggtga agagagcaac ggcaacccgc
     9901 tgatctactt tgcggttgca acggttctgg aactggtgtt tggtattctg gcgagcatta
     9961 tcaccatgtg gttctcgcgt catcgtgaat tccatgctga tgccggttcg gcaaaactgg
    10021 ttggtcgcga gaaaatgatt gccgcgctgc agcgcctgaa aaccagctat gaaccgcaag
    10081 aagcaaccag catgatggct ttctgcatta acggtaagtc gaaatcgctc agtgagttgt
    10141 tcatgactca cccgccgctg gataaacgaa ttgaagctct gcgtacgggt gaatacctga
    10201 agtaatatga agtaaatact aaaaagcgcg tcctgatcga cgcgcttttt ttatgccttg
    10261 gatcgtggct gagtgatacg taaaccactg acacaggcag caatcactgc cagaatagcc
    10321 gcagccatca gcgaaacgtg cgtaccatta tcaccgaact gatttagcat cagcgccacc
    10381 agtgccgcgc cgctactctg acccagtaga cgagccgttc ctaacatgcc actggctcca
    10441 ccgctacgtt cgcgaggagc ggaggtaata atggtgtggt tattgggtga ctggaacaag
    10501 ccaaatccag cgccacataa gatcatcggc cagataatat tgatatccgc aggtgacgcg
    10561 ggcagcagaa ccaggggaaa aagccccgca gccatgatga acaaccctag agcacccagc
    10621 aatcctgcat gtacgcgttc aatcaaatag cctgccaacg gagccatcac catcgttgct
    10681 aacggccacg gtgtcagaag taaacctgtt tcgacttcac tacgcccgag cacggtttgc
    10741 aggtaaaagg gcagggaaac cattgccagc atttgtgcgc agaaagagca aacagatgtg
    10801 caaatagaaa gtgaaaacag cgggatacgc agtaaatcca ccggtagcag cggtacggga
    10861 agagaaagct ggcggcgaat aaagaaaata ccaacaacaa ccattaccac cagttccgca
    10921 gcaattaacg tcagcgattg cccctgagcg aaaccactca acgcagtgat aagcaggccg
    10981 aaggttaacg cgttcatcac ggcgctgggc aggtcgaaac ggggtttact ggcgcgagaa
    11041 ccattgggtg gcagaaaacg catcgccaga agcagggcga taatacctaa cggtacgttg
    11101 attaaaaata accatttcca ggatgcgatg gagaggattg ctgcagcaat tgtcggcccg
    11161 gcagcagaag agacggcaac aatatggcga ttgttggcag ggcgacgttg gcgattgcgc
    11221 cgtcaaggac agccatcgaa atgccaatca caatggttaa tatcgcaccg tatcgctggg
    11281 gcaatggcag gccgtcggcc tgaacttttg gcataggaat tttatatctt tggtgaataa
    11341 tcaacagggg tatgattcta gcactgatat tcacggtgga tgtcgcagaa ttgtaactaa
    11401 ccggcaaaag atggattgcg accaccctgg tgcgaaatta taataaaaaa cagttctgat
    11461 ttttataaaa cactcgcaat gaggtgataa atggctaacg cagatctgga taaacagcct
    11521 gattctgtat cttccgtgct aaaagttttt ggcattttgc aggcgctggg tgaagagcgc
    11581 gaaataggga taaccgagct gtcgcagcgc gtcatgatgt caaaaagcac cgtttatcgc
    11641 tttttacaga ccatgaaaac cttaggttat gtggcgcagg aaggggagtc ggagaaatat
    11701 tccctgaccc tgaaattgtt tgaactgggc gctcgcgcgt tacaaaacgt cgatttaatt
    11761 cgtagcgcag atatccagat gcgtgagatc tcccgcctga ccaaagaaac tatccacctc
    11821 ggcgcactgg acgaagacag tattgtttac attcacaaaa ttgactctat gtacaatttg
    11881 cgcatgtatt cacggattgg gcgtcgtaat ccgctgtaca gcaccgcgat tggtaaggta
    11941 ctgctggcat ggcgcgatct cgatgaagtg aagcaaattc ttgagggcgt ggagtataaa
    12001 cgcagtaccg agcggaccat caccagtaca gaagcgttat tacccgttct ggaccaggtg
    12061 cgcgagcagg ggtatggcga agataacgaa gagcaggaag aagggctgcg atgcattgcg
    12121 gtaccggtat ttgatcgctt tggcgtggtc attgccggtt tgagcatctc cttcccgacg
    12181 ttgcgtttct ctgaagagcg tttacaggaa tatgtcgcaa tgttgcatac cgcagcgcgc
    12241 aaaatttctg cccaaatggg ttatcacgac tatccgttct gatgagagta agaacctgtc
    12301 ggaatatcaa acagacaggt tctttattta gcatgagaaa aataaagttg aaggtgcgtt
    12361 atattaaacg cgcttgctat aagaatattt tactcatgag tgagaatctg gttgtttatt
    12421 acccttaacc attatcgacc acgatattgc ttttacgtaa cagtgggcaa tctgttatcc
    12481 ccaagaaacc acttttagtg tgcaagtatt gtaccgtgct ggtgcctctg gcagtcagat
    12541 aggtacattg caaacctaat cctgcggcat tctctttgct tccaatcaaa acgccatatc
    12601 cgctgagtaa taatcctatc cataccagtg ctatcagcat aactgtgcga atgatgaatc
    12661 gcattacaac ctcttctctt tttatgttcg cttaatcgta gcggcaatat gcgctgaagc
    12721 aagctactca ttccgaaaaa gcacgaatat cgacatagtt aggcgctgtt taactaacgc
    12781 atgctagttt aatgacataa ggtaggtgaa gcggagattg gagtgaaaaa gtttcgatgg
    12841 gtcgttctgg ttgtcgtggt gttggcttgc ttgctgcttt gggcgcaggt attcaacatg
    12901 atgtgcgatc aggatgtaca atttttcagc ggaatttgtg ccattaacca gtttatcccg
    12961 tggtgatatc attttttcag cactagggaa ggtgcgaata agcaggtcat ttcttcccaa
    13021 gctgactcgc tgattaaaat ttcgcggatc tgggccgatt tttttcccgt aaacacatcg
    13081 aatcagccta tttaggctat tttttccacc atttctggcg ttatttccgg tttttactga
    13141 gatctctccc actggcgtat catttggtcc acccgaaaca ggttggccag ggtgaataac
    13201 atcgccagtt ggttatcgtt tttcagcagc cccttgtatc tggctttcac gaagccgaac
    13261 tgccgcttga tgatgcgaaa cgggtgctcc accctggcac ggatgctggc tttcatgtat
    13321 tcgatgttga tgaccgtttt gttcttgcgc ggatgctgct tcaaggtttt taccttgccg
    13381 ggacgctcgg cgatcagcca gtccacatcc acctcggcca gctcctcgcg ctgtggcgct
    13441 ccttggtagc cggcatcggc tgagacaaat tgctcctctc catgaagcag attacccagc
    13501 tgattgaggt catgctcgtt ggccgcggtg gtgaccaggc tgtgggtcag gccactcttg
    13561 gcatcgacac caatgtgggc cttcatgcca aagtgccact gattgccttt cttggtctga
    13621 tgcatctccg gatcgcgttg ctgctctttg ttcttggtag agctgggtgc ctcaatgatg
    13681 gtggcatcca ccaaagtgcc ttgggtcatc atgacgcctg cttcggccag ccagcgattg
    13741 atggtcttga acaattgacg ggccagttga tgctgctcga gcaggtggcg gaaattcatg
    13801 atggtggtgc gatccggcag ggcgctatcc agggataatc gggcaaacag gcgcatggag
    13861 gcgatttcgt acagggcatc ttccatggca ccgtcgctca ggttgtacca atgctgcatg
    13921 cagtgaatac gcagcatggt ctccagcgga tagggccgtc ggccattgcc cgccttggga
    13981 taaaacggct cgatgacagc ggtcatattc tgccatggca gaatctgctc catgcgggag
    14041 aggaaaatct cttttcgggt ctgacggcgc ttagtgctga attcactatc ggcgaaggtg
    14101 agttgatggc tcatgatgtc cctctgggat gcgctccgga tgaatatgat gatctcatat
    14161 caggaacttg ttcgcacctt ccctagattt tcttcctctc cagcagtggt agaatagcgc
    14221 ctctactcag aggtggtgaa atgaacgaag ttgtaaattc aggcgtgatg aacattgcgt
    14281 ctttggttgt atcggtggtg gttcttctta tcgggctcat cttgtggttt tttatcaatc
    14341 gtgccagttc acggactaac gaacagattg aactgcttga ggcgttgctg gatcagcaaa
    14401 aacgtcaaaa tgcactgtta cgtcgtttgt gcgaagcaaa cgaaccagag aaagcagata
    14461 aaaagaccat tgagagtcaa aaatcggttg atgacgaaga tattattcgc ctggtcgccg
    14521 aacgataatc cttcctcaat cttgagtaac cccgtcgttg aagggttact caattcatcc
    14581 tgattatacg cactgtcatg gtgcaactgc ttcaaattaa aactctggtc tgacttcaaa
    14641 taattatcat attatttatt tgcgtaacgg gatatcagaa aaagatattt tcctcattaa
    14701 tttattagga tgtttacatc ggatttgtga ttaagcgtgg tattatttat tagcgaaacg
    14761 tttctctctt gatttttttg ctcattcatc aatttttctt attttaaatt ttcaatccct
    14821 ttggggattg acttctcttt agggtaattg atagccgtta accgactgtt ttatgagaaa
    14881 aagtgatata actttttatt cattgcatag caaaaaatgt gatattgcac gcactatgta
    14941 ataacttctc ccactggcct ggaacaactg aacttattga actatgttag aaaatacgcc
    15001 agtttaagta tctgcctgaa ctggcaaggt taagcacaat gatatatcgg cgcgtattcc
    15061 gttgcataag tgtgcaaaaa aagtggaaga cgtatcgaga tttgtgcgtc tgatcgagac
    15121 atgtttaaaa atggcttgcc ataattaacg ttgtatgtga taacagattt cgggttaaac
    15181 gaggtacagt tctgtttatg tgtggcattt tcagtaaaga agtcctgagt aaacacgttg
    15241 acgttgaata ccgcttctct gccgagcctt atattggtgc ctcatgcagt aatgtgtcag
    15301 ttttatctat gttatgcctg cgggcgaaga aaacaatcta aggaattttt caaatggcaa
    15361 agattaaagg tcaggttaag tggttcaacg agtctaaagg ttttggcttc attactccgg
    15421 ctgatggcag caaagacgtg ttcgtacact tctccgctat ccagggtaat ggcttcaaaa
    15481 ctctggctga aggtcagaac gttgagttcg aaattcagga cggccagaaa ggtccggcag
    15541 ctgttaacgt aacagctatc tgatcgaatc cactgatctg aagtgtgaat acgcttcaat
    15601 ctcgctataa agcctcgtcg aatgcgaggc tttttactat gctttatctt cgctcctggc
    15661 gttcggatat ttgcccgccg cgtgattcgc gttacacttg cggcctttag tatcctgccg
    15721 gagttgtcat gtctttttcc tgtccacttt gccatcagcc tctttcgcgt gaaaaaaaca
    15781 gctatatctg tccccagcga catcagtttg atatggcgaa agaagggtat gtcaatctgc
    15841 tgtccgttca gcataaacgg tctcgtgatc cgggcgacag cgcggaaatg atgcaagcac
    15901 gccgcgcatt cttagatgcc ggacattatc agccgctgcg tgatgcaatt gtcgcccaac
    15961 tgagggaacg gcttgatgat aaggccacgg cggtgctgga tattggctgt ggtgaagggt
    16021 attacacaca cgcatttgcc gatgcgttgc ccgaaatcac cacgtttggt ctggatgttt
    16081 cgaaggtagc gataaaagcg gcggcgaaac gctatccgca ggtcactttt tgtgtcgctt
    16141 ccagccaccg tttgccgttt tccgatacca gtatggacgc cataatacgt atttacgcgc
    16201 cgtgtaaagc agaagaatta gcacgagtag tgaagcccgg cggctgggtc attactgcca
    16261 cgccgggacc gcgacatttg atggagctga aggggctgat ttacaatgaa gtacatcttc
    16321 atgcacctca tgcagaacaa ctggaaggtt ttacattaca gcagagtgcg gagttgtgtt
    16381 atccgatgcg tcttcgcggt gatgaagccg tcgcattatt gcagatgacg ccgtttacct
    16441 ggcgtgcgaa gccagaagtc tggcaaacac tggcagcaaa agaagtgttc gactgccaga
    16501 cggactttaa tattcacctc tggcagcgtt cttattaacc gtggaagtgc gtccagagga
    16561 tctggacgcc gatgccgatc agtaccagcc cgccgagaat ttccgctttt ttcccaataa
    16621 ttgagccgat aaagcgacca accatcatcc ctaatgttga cataatcaag gttgcacaac
    16681 caatggccaa tgcggtcgcg ataatgttga cctgcaggaa agcaagacca acacccacag
    16741 ccatggcatc caggctggtg gcaatcgcgg tggttaccag tagccagaaa ccgtgtcgac
    16801 ggcgcggctc ttcatcttca tcatctgcgc cacgaaaacc ctcaataatc attcgcccgc
    16861 cgaggaatat cagcagcaca aacgcaatcc agtggttcca ttcaaggaca aaccggctgg
    16921 ctaacatgcc cattccccag ccgatcagcg gcgtcagggt ttcgacggca ccaaaaataa
    16981 ggccggttcg caatgcttca gaaaatttcg gtttatggag ggtggcacct ttaccgattg
    17041 atgcagcaaa tgcatccatc gacataccaa acgcaagaag aacagtagca gtgatattca
    17101 tgacaatgtc ctgaccgggg tatccataaa acacatcatc gcccccagca aacatgcata
    17161 catgaacaac aagccgtgat ggttttcgcg ttcatgcagg catagcgatt gatgtgcata
    17221 tggtctcgcc tgactgaaag tattcagtcc gtacgtgcca cgttttgcaa cgagtatgtt
    17281 gacacgtaca tttccggaat ctggaaatcg gctactcccc aaatgagggc gcaaccttaa
    17341 catattttga gaatataaaa caactgttgg aatatattat ttcattgctt tattgataac
    17401 gattttcatt tggatttata tatcaatgaa acgttaaaat aaataacaaa taagggaaga
    17461 ggtaatatag cctaagctat gtctgattat taaaaataca ctaaacatag ctttggctaa
    17521 attcataact tattgagttg aaacgagaag cttataaatt ttttccagat cgtcgatatt
    17581 tcgaacgcgg attaacagcc gacgctgttc taattgcatc accaacacgc catcttccga
    17641 caagttcatc gctttgattc ggctatattc aatccagaca ttggcgaaga aaaaaccttt
    17701 ttgtttaaag atgatcttcg gaacgcggat ccagaatata taaaaaccca tcagagccag
    17761 tgcgcttaat aaccatgtcg ttattaacgc accatgattc gtgacgttgt tatagataag
    17821 aatgacaatc aatccgacga agataacgct atcgatgcga ccacgccgga gcaaaggaat
    17881 tgccagcagg gtggggccgt tacggcgggg catgatgaac tgatcgtaga tcgcgaaggc
    17941 caggagtgcg gcgatgaaaa gaatcagtac caggtccgtg attgtcatta atcctccaga
    18001 taaaaaaacg gggccaaaag gccccggtag tgtacaacag tcttacagtc ccagcaggcc
    18061 gcaagcgtaa ccagcgatac cgatgacgaa gaagccaacg atgatccaca gcgggttaac
    18121 ttttttgcgc agtagccaca tacaagcaaa ggtcagcagc agtggtacca ggcctggcat
    18181 taactggtcc agaatagtct ggacagtagt aacgtgttct ttgcccgtct ggtcagtaat
    18241 gcgagagaca accagcggga tgttgacatg tgtccacttg ttaaccaatg cccccatgac
    18301 aaacaggccg aggatagacg ccccttccgt cagtttttgc aggaagccac cacccatatc
    18361 tttaacgata tcgatacctt tggagtaacc atacgctacg ccgtagtaac gggttgccag
    18421 acgcaccagg ttaaacagga tgaagaacag cagcggacct aacaggctgc cgctcatcgc
    18481 gataccggca cccagtgctg caaataccgg acgtacggtt ccccagaaga tcgggtcgcc
    18541 tacaccagcc agcggcccca tcaaaccgac tttgataccg ttgatagcac cgtcgtcgat
    18601 ctctgcgcca ttagcacgct gttcttccag cgccagggtt acgccgagaa tcggcgcagc
    18661 cacgaacggc tgggtgttaa agaactccag gtgacggcga atagcttgtt tacgagcttc
    18721 gttgttctca gggtagaggc gacgaattgc cggtaccata gagaagcaga aacccagtgc
    18781 ctgcatacgt tcgaagttcc atgaaccctg gaagaggtta gaacgcagga agacgccacg
    18841 aatatcactt tgagtgagtt ttttctcggt ggtagtttga gttgtatcaa ccatttcgct
    18901 cacctgttag tccagttcgt tatcgagatc gttgttacca gctgcctgag caggcgcacc
    18961 ggctacgcgg ttgtatttcg ggctaagttg gatgtagagc actgccataa cagtaccaat
    19021 cacacccaga gcaaccaggt taaagttggt gaatgctgcg gttacgaagc cgaggtagaa
    19081 gaacggcatc aggtagccag cacgcatcat gttgataacc atcgcataac caaccaccac
    19141 gatcatgcca ccggcgatat tcagaccatt ggtcaccact tccggaatcg cattcagcat
    19201 gttctgtact tcgctggtac caacagacag cgcaacgatg acggccggaa tagccacacg
    19261 cattgcttgc aggaacagag aagaaacgtg gatccaggaa atcgctgtca ggttgccgtt
    19321 atcagcagcc ttatccgcag cgtgctggaa agcaacggta atagtacgaa cgatgatggt
    19381 cagtacctgg cccgcagcgg ccagagggat tgccagtgcg atacctgcac caatgctctg
    19441 atgacctgcg ataaccagaa tggtagaaat gatagaagcc agagcggcgt caggcgcaac
    19501 tgcagcaccg atgttcatcc agcccagcgc gatcatttcc agcgtaccac cgataataat
    19561 accggttttc atatccccaa gaacgatacc caccagggta cacgcgatta gcggacggtg
    19621 aaactgaaat tcatcgagga ttgatcccat acctgcgata caggctacga taaataccag
    19681 cacaatttga agagtggtaa tctccattgt acttctcctg tttacgtaag acttaagtgt
    19741 gaaaactgag tgataatcaa cacaatacgt tacttatcga ttttgctgat cagatccatc
    19801 attttcagtt tcggatcggt ggaaacctta cggacttcca gctcaatacc gcgcgcattc
    19861 agtttcttga acgcctcgat atctttttca tcaaccgaaa ccgcgttatt cacctgggtt
    19921 ttaccctgac ggaatgccat accaccgacg ttaacagagg tgattttcac gccgccttca
    19981 acgagacgct ctacatctgt tgggttggta aataacagca ttacgcgttc gccagcatat
    20041 ttcgggttgt tgtagacgcg aatcattttg gcaacatcaa ctacgtgtgc tgttacgccc
    20101 ggaggtgcaa cctgggtgag cagtgtctta cgaacggtat ccgcagccac ttcatcacta
    20161 acaacaataa tacgggagac attggtttct ttggtccagc gggtggcgac ctgaccgtga
    20221 atcagacggt cgtcgatacg cgcaaggcca ataaccatgt agtcgtttgg ccccattggt
    20281 tttgccggag ttggagccgc ttttggtgct gcggcagcgg gtgctggcgc ggctttttca
    20341 accggtttgg ctttcagtgc tttcacgcct tcacggcctg tttctactgc cagcgcaacc
    20401 agttcatcaa agcttgggtc atcatcacga gccattaacg tttccacgag cattggaatg
    20461 ttaacgcctg caatgacttc ataatgctct ttgtcgacga caatgcggct ggcagcattg
    20521 aacgggctgc ctccccatgt atcaacgaga aacagcacgc ctttagtggt gtcgagtttt
    20581 gccaactgag cgttgtactt ttcaatcagc gtttcggcat tttcacctgg aacgaaatcg
    20641 atccagccga cgttttcctg ctcgcctaac agcatttctg ccgttttaag caactgctct
    20701 gcagcccaac catgtgtgcc tataacaata gcaatggtca cttgctacct cctttattat
    20761 cgttaacacc tcaacgtgcc agatgtattt ttgaatcgtc gtccacaatc gaatcgattc
    20821 agataagggc tacaaccaaa aacccctgcg tttcgcgatt tattttagat atcgaaaaaa
    20881 ttattttatg tgatgaagat ccgtaattta actttcgatt agcagaaatt tcgaaaggta
    20941 aaatatcctt gtcacattcg tttgcaaagg aaggtaaatc tttgccaaat cagaggcgtc
    21001 tctgatatgt ttaactcccg tttaacaacc atggagtata gggcagtagc ccgcagtatg
    21061 gatcgtcacc gacgtcattt cagcatcagg ccttttaacg cctgcctttc tggcactcta
    21121 tgccgcacct ttcgtttgca ttttgtcgtt acgcctgcat tatttctggc gtcgaatagc
    21181 tattccttaa gcaggagctt gtcatggaat tcttaatgga cccctcaatt tgggcggggc
    21241 tactcacgct tgttgttctc gaaattgtgc tgggtatcga taacctggtc ttcatcgcca
    21301 ttcttgctga caaactgccg ccaaaacaac gcgataaagc gcgtttgctg gggttatcac
    21361 tggcgctgat tatgcgtctg gggctgctgt cgctgatttc atggatggtc acgctgacca
    21421 aaccgctatt taccgtcatg gatttctcct tctccggacg cgacctgatt atgttgttcg
    21481 gggggatatt cttgctgttc aaagcaacaa ccgaactgca tgaacggctg gaaaaccgcg
    21541 atcatgattc cggccacggt aaaggctacg ccagtttctg ggtggtcgtc acacagattg
    21601 tcatccttga cgccgtcttc tcgttggatg cggtaattac tgcagtaggg atggttaacc
    21661 atctgccggt gatgatggcg gcggtagtga ttgcgatggc ggttatgttg ctggcatcca
    21721 aaccgctgac gcgattcgtt aaccagcacc ccacggtggt ggtgctctgt ctgagcttcc
    21781 tgttaatgat tggtctgagt ctggtggcag aaggtttcgg tttccacatt ccgaaaggtt
    21841 acctgtatgc cgcgattggc ttctcgatca tcatcgaagt gtttaaccag attgcgcgtc
    21901 gcaactttat tcgccaccag tcgactttgc cgctgcgagc gcgtactgcc gatgccatcc
    21961 tgcgtttgat gggcgggaaa cgtcaggcca atgttcagca cgatgccgat aacccgatgc
    22021 cgatcccgga aggtgcattt gccgaagaag aacgttacat gattaacggc gtactgacgc
    22081 ttgcgtcgcg ttctctgcgc gggatcatga cgccgcgcgg tgaaataagc tgggttgacg
    22141 ctaatctcgg ggtcgatgaa atccgcgagc aactgctctc ttcaccgcac agtctgttcc
    22201 cggtatgtcg cggtgaactg gatgaaatca tcggtattgt acgtgctaaa gaactgctgg
    22261 tggcgctgga agagggcgtt gatgtggcgg cgattgcttc ggcgtctccg gcgattatcg
    22321 tcccggaaac cctcgatccg atcaacctgt tgggcgtgct gcgtcgtgct cgcgggagct
    22381 ttgttatcgt gaccaacgag tttggtgtgg tacaaggtct ggtcacgccg ctggatgtgc
    22441 tggaagccat tgcgggtgaa ttcccggacg ctgacgaaac gccggaaatc attactgatg
    22501 gtgacggctg gctggtaaaa ggcggtacag atttgcatgc cttgcagcag gcgcttgatg
    22561 ttgagcacct tgccgatgac gatgatatcg cgacggtcgc gggcctcgtg atctcggcaa
    22621 atggtcacat tccccgtgtg ggcgatgtga ttgatgtagg gccactgcat atcaccatca
    22681 ttgaagccaa tgattatcgt gttgatctgg ttcgcattgt taaagagcaa ccggcgcacg
    22741 atgaagatga gtaagcatta acgtaacggc ataatgggcg tgatatgtcc attatgccgg
    22801 gcgggcggcg gttggctgcc cgccagccat ttgggaaaat cacgtagcgg catcggctgt
    22861 gcgtacaaat acccttgcaa aatatgcacg ccatgacggc gcaaatactt cgcctgttct
    22921 tgcgtttcca cgccctccgc caccagttca atattcagtc tttgccccag cgcgatgatg
    22981 atatcggtca ccgtcgagtt aaccgcgtca gaacctatag ctgcggtaaa tgacttatca
    23041 attttcagca tgtcaggacg taatgtttca agccaggaaa atgaactgtt gccggtgccg
    23101 aagtcatcaa tcgccagttt gacgttttta cgatgtagct cgcgggcaat ccgataatca
    23161 acatccagta aggcatcgcg ttcggtgatt tcgaggatca gttgctgaat cgggtgagcg
    23221 ctaaaccagt actgattgag atcttttatc agcacaccac ggcgaaaatg gctgggtgcg
    23281 acgttaatgc caacatgaaa ctgactactc atcgggaaaa catggcgctg acgaatggtt
    23341 tctgccatca catagcgggt cagtggcaca attaaatgat gttcttccgc gataggaata
    23401 aacacatccg gtgaaatcca gccctgacgc ggattgttcc agcgcagcaa aatctctaca
    23461 ccaatacatt gctggctgcg cgcattaagc aaaggctgac agaacaattc gaactcatgt
    23521 tgcgccaggc ccagattgat ttcgcgggaa aagctcatcc ggtaagcggt cgccagccag
    23581 gcgatgtagc ccaccagtaa acttagcagc accgccagcg gtaattgtgt tggaagatag
    23641 tgccatgcca gcgccgtagc ccccggacca ttaacgttaa tggtaaaagg aaagcgttgc
    23701 gaagaaacct gatagttttc attgtcttca ggttgcggaa gggaatctac cagcccatta
    23761 ccatagagca aatgccgttt gtccaccgtc aggctggcgc tactgatttg cggcagttgt
    23821 ggctcaagga gcattgccgt cagtaagtcg atgttaatca tctccatgac cccagcattg
    23881 ctactgcccg ctgcaggcgt ccattgaatc aaaaccggac tgcctttaat cagggcacgg
    23941 tcgatcgtca ggcgtaaaag tggttgcggt gcaggaagtt cagccagaat gtccacgacg
    24001 ggcacattgc gataaccaaa aatgctggag caatagagtg ttccgtcttg caccaggcca
    24061 atggatcgca cagtttggag ttttgccgcc tgtttacgta atggcaaatg ggcgacagag
    24121 cagggcagac caatcagcgg aagcaagact tcgctacctg cctgtagggg aagcagtact
    24181 ttatctaatt cctctacagc gtgattggcg aattgtacta cccgttgttg atttaagtta
    24241 cgctgtgaaa taaatcgcac gctcagggtc gaagcgagcg taacgagtgc gcaaatcgta
    24301 caaacaatca ttcgattacg gcgataggtt ttaatgatcc gttgtgcttt ttgcatgcgg
    24361 gccaccctgt aagcgtatgg cgacaaacgc ctgacgcaac agtggaagtg tagtggggaa
    24421 tattacagca gacgagaaag cgggtataaa ttcgcccatc cgttgcagat gggcgagtaa
    24481 gaagtattag tcacactgga ctttgattgc cagaccaccg cgtgaggttt cgcggtattt
    24541 ggcgttcatg tccttaccag tttcgtacat cgtttcgatg accttatcca gcgagacgcg
    24601 cggtgcactg gtgcggcgca gagccatccg tgcggcgtta atcgccttca cagaggcaat
    24661 ggcattacgc tcaatgcacg gcacctgaac ctgccctgca accgggtcgc aggttaaacc
    24721 aaggttgtgt tccatgccaa tttccgccgc cacgcaaacc tgttccgggc taccgcccag
    24781 cagttctgca agacccgcag cagccattga acaggcaaca cccacttcgc cctggcaacc
    24841 aacttccgca ccggaaatag aggcgttcat tttatacaat gcaccaatcg cgcccgctgc
    24901 cataaagtaa cgggtataga tgtccgggct gaccgattca ataaagtggt catagtaagc
    24961 cagcactgcc ggaacgatac cgcaggcacc gttggttggc gcagttacca cacgaccacc
    25021 ggcggcgttt tcttcgttaa ctgccagcgc aaacatgttt acccagtcaa tgacattcat
    25081 cggatcgtta gacagtttat cgctggaaac taacatccgg cgcagggcag acgcacgacg
    25141 tggcacgcgc agcgggcctg gcagtacacc ttcggtgttc atcccgcgat cgatacatgc
    25201 ctgcatggtt tgccagacat gcgcgaaata ctcgtcgatc tctttcttgc tgtgcagcgc
    25261 cagttcgttc tgcatagcga gaccagacag cgaatagccg gtttcattac agtacgcgag
    25321 cagttcggtg gcagatttga acggatacgg cacgctcact tcgttggcag catcctgacc
    25381 aaagtgttct tcatcgacga taaaaccgcc gccgatggaa taataagttt tgctgtagac
    25441 gacttcatcg ccgttatagg cgtggatttg cataccgttt tcatgcagcg gcaggttgcc
    25501 gttatgaaaa cgcatcccgt tgtcgcgcgg gaaatccact tcatgccgtc cctgtgccag
    25561 cagcagacgt tcgcgctctt ctacgtcgcg aataaaaccg ggaatactgt cgatatccac
    25621 ggtggcaggt tcgttacctg caagacccat aataatggcg atatcggtgt ggtggccttt
    25681 acccgtcagc gacagtgaac cataaacgtc cacggcaacg cgagtaacgc tatccagtaa
    25741 gcctttttcg accagatcat cgacgaactg tttacctgcc ttcataggcc ctacggtatg
    25801 ggaagatgag ggaccaatcc ccaccttaaa catgtcgaat agactaatca cgataatact
    25861 cctgacaagg tgactggact tccagtaacg aactaataac agcgcatagt gtaagaggga
    25921 acgcggcgac tggcttaact attcacatga attaaactaa tcaaaaaccg cgatttaact
    25981 aatattgcga tgattgtctc aagtagtgtt ttgtagatgt aaagataagt atagtcaggg
    26041 tttcacacca atttgcagcg ccagctcacg aattatgcct gcggtcattc cccatacaaa
    26101 atactgttcg taccaggaca gccatacccg atgtgaatcg ccacggcggt agatatctaa
    26161 agggtgataa cgacccagat gtaatgcctg ggcgagcggc atttcaaaca ccgccgagac
    26221 ttcatcttca ctggcgcgat acggcagatc gggcgggata atgccgacca ctggggttac
    26281 ctgatagcca gttacgctat cgacgggcgg cagcacgccg ataacttcaa cggcggaagg
    26341 cggtatagcg acctcttctt cagcttcgcg cagcgcggcg gcgataactg atgcgtccgt
    26401 gtcatcgact gcacctccag ggaatgccac ttgtccagcg tgtttacgca gatgaattga
    26461 acgctgagtc agcaacaacc ccggttgcgg tcgacggacg atggggatta acacggcagc
    26521 ctgacgatga tttagggttt cccggttaat ttgcgggcgc aaaagttgaa agcgcgataa
    26581 aaaatcatca agcatcaggc tacggtattc cacgtcttac ttctccagtt ggcgtaatat
    26641 cttattaact ttatcaaaag tttcctgata ttccgcttct tcctggctat cggcgacaat
    26701 tccaccgccc gcagagcagt atatttgtcc gttaatggca gtcagagtgc ggatagtaat
    26761 gctggtatcc atgttgccgc aaaagctcaa atagccaatg ctgccgcacc aggcattacg
    26821 tcgctgcggt tccagttcgt cgataatttc catagcccgt actttcggag ccccggttat
    26881 tgagccacca ggaaaagctg cgcgcagcag atcgctggcg tgtaactgtt ctggtagttg
    26941 tgccgttatg gtgctgacca gatgatgcac ggcagggaag ggttccacca cgaacagctc
    27001 tggtactttt accgaacctg ctacggcaac acgaccgata tcattacgca ttaaatcgac
    27061 aatcatcaga ttttcggcac gatctttcgc tgagttcgcc agtttttctg cttgtttgct
    27121 atcttcctga ggatcgggca ggcgtggtag cgtgccttta atagggcggg tctggatttc
    27181 actattatca caaagaataa accgctctgg cgaaaggctt aaaattgcac cctgttcaag
    27241 acgtaaaaaa gcgctaaatg gcgcgcggtt ggcctgatta agctgaagga atgcctgcca
    27301 ttcatcgcca gaataggtcg catgaaaacg ctgggcgaga tttacctgat agcaatcacc
    27361 gctgtgcaga tattcctgta cctggcgaaa tttttcgccg tactgctcgc gggtcatatt
    27421 ggattgccag tcggaagtaa gcgtgaaatc ttcctgcggc gagaattgct ggctttccag
    27481 ccaggcccga cgggcattga catcattatg actcagcaaa gaaactgtat gacgctggtg
    27541 gtcgacaatg agcgcccaat cgtagatacc cactgccata tccggcagaa cgatatcttg
    27601 ttccgcaatt tctggcagtg actcaaaacg gcggcccaga tcgtagccaa acaaccccag
    27661 tgcgccgccc tgaaatggca aatcttcgtt atgcgttggg cgaatgtctg cgcgatccag
    27721 cacctgctgg agcacctgta gcgggtcatc agtggtcgtt gtgcgttttt cgctttcact
    27781 aacaacggtt tctttaccta aagtggttaa agtgcaaatc ggatcggcga ccacaatatc
    27841 aaagcggcta tacggatgat cggcatagcc ggagtgtaaa agcatcgccc acggcaggtg
    27901 gcttaagcgg gagaaataaa attcagcggc gtcctgacgc cagggtaaag taatcacagc
    27961 gggagataac gtcttcatca gtcctgactc tactggctat gtgccgggta gcatgaaata
    28021 attagcgcca acaatttagc aggagttaac aatgtttgca ggtttacctt cactcaccca
    28081 tgaacagcag caaaaagctg tcgagcggat ccaggaactg atggcacaag ggatgagcag
    28141 cggccaggca atcgcgttgg tggcagaaga gctgcgtgcc aaccattccg gcgagcgaat
    28201 cgtggcgcgt tttgaggatg aagacgagta acacactaat ccgtattgat ttcgttatac
    28261 aagccgatga ttatttgatt gtgagaaaaa ggacgctttt tatatgtgat taaaagcgtc
    28321 ctgatccatt tatgctgatg taaattttac tcagaccagt gcttttagtt cttcctgcat
    28381 atatgttgaa aagtattcag gatttttaat cacagagcgt tcacctgact ccattttttt
    28441 tgcccatcca accatttttt ccgtaaaccc agggttccag ccttcctggt cagctcgcgc
    28501 cgtaatatca gcggcactcg caggtactcc cagttctttt aaatatttaa agattccctt
    28561 cgccgtgctt tcattcatcg aacttggtgt tggcgctgat acatccatgg caccaatgaa
    28621 atccagggct ttatctatta cagcaggcat attcttatcc ttaaaattaa ccatgttatc
    28681 gataaggcta tatatgacct ta
//
LOCUS       DADMKF010000041        28678 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.042, whole genome shotgun sequence.
ACCESSION   DADMKF010000041 DADMKF010000000
VERSION     DADMKF010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28678
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.042"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(<1..1046)
                     /locus_tag="JNC24_001620"
     CDS             complement(<1..1046)
                     /locus_tag="JNC24_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein"
                     /protein_id="HAY9158386.1"
                     /translation="MREINMLRNISSCLFPHISTITSPNHYLSEWDDWEKQGLPEEQR
                     TEAVRRLRACLTSKGHKLDLRALALSSLPVLPACIKKLDVSCNKLTILTDLPENIKEL
                     IARDNFLTHISALPHYLITLDVSENQLENLPLLPDTIKSLSAEYNRLSTLPSLPLNLK
                     KLEVRNNELQTLPSLPSNLKILKVAHNHLTELPPLPRRLQLLFAYSNRLSNLPNIQEN
                     IIMRRFFYFENNQITTIPTNLFRLDPHITIEIANNPLSDQTLLFLIQQTSVPNFNGPQ
                     FRISLSDQNRLFLRQMLPQNLHSRHIRVITEGGQNFQIPPLPETVAAWFPEADRREVS
                     TQWTSFSTEENSRA"
     gene            1537..1935
                     /locus_tag="JNC24_001621"
     CDS             1537..1935
                     /locus_tag="JNC24_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708399.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAY9158387.1"
                     /translation="MLIGYVRVSTNDQNTDLQRNALNCAGCELIFEDKISGTKSERPG
                     LKKLLRTLSAGDTLVVWKLDRLGRSMRHLVVLVEELRERGINFRSLTDSIDTSTPMGR
                     FFFHVMGALAEMERELIVERTKAGLEAARA"
     gene            complement(2433..3281)
                     /locus_tag="JNC24_001622"
     CDS             complement(2433..3281)
                     /locus_tag="JNC24_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAY9158388.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAAVRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            3490..4125
                     /gene="yfhb"
                     /locus_tag="JNC24_001623"
     CDS             3490..4125
                     /gene="yfhb"
                     /locus_tag="JNC24_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HAY9158389.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            4150..4686
                     /gene="tadA"
                     /locus_tag="JNC24_001624"
     CDS             4150..4686
                     /gene="tadA"
                     /locus_tag="JNC24_001624"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HAY9158390.1"
                     /translation="MRRAFITGVFFLSEVEFSHEYWMRHALTLAKRAWDEREVPVGAV
                     LVHNNRVIGEGWNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCA
                     GAMIHSRIGRVVFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFF
                     RMRRQEIKAQKKAQPSTD"
     gene            complement(4683..6239)
                     /gene="mltF"
                     /locus_tag="JNC24_001625"
     CDS             complement(4683..6239)
                     /gene="mltF"
                     /locus_tag="JNC24_001625"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HAY9158391.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVSQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKDTKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQCLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            6497..10384
                     /gene="purL"
                     /locus_tag="JNC24_001626"
                     /gene_synonym="purI"
     CDS             6497..10384
                     /gene="purL"
                     /locus_tag="JNC24_001626"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HAY9158392.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFALDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFKMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYDFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLSTEDNTLLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVAQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGINADIASLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFHRAGFDAIDVHMSDLLTGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            10942..12369
                     /gene="qseE"
                     /locus_tag="JNC24_001627"
     CDS             10942..12369
                     /gene="qseE"
                     /locus_tag="JNC24_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HAY9158393.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFSGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSRNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSS
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            12534..13247
                     /gene="qseG"
                     /locus_tag="JNC24_001628"
     CDS             12534..13247
                     /gene="qseG"
                     /locus_tag="JNC24_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HAY9158394.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQNHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPTGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            13237..14571
                     /gene="glrR"
                     /locus_tag="JNC24_001629"
     CDS             13237..14571
                     /gene="glrR"
                     /locus_tag="JNC24_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HAY9158395.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDINVRIISAT
                     HRDLPKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNQFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            14632..14970
                     /gene="glnB"
                     /locus_tag="JNC24_001630"
     CDS             14632..14970
                     /gene="glnB"
                     /locus_tag="JNC24_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HAY9158396.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(15015..16205)
                     /gene="hmpA"
                     /locus_tag="JNC24_001631"
     CDS             complement(15015..16205)
                     /gene="hmpA"
                     /locus_tag="JNC24_001631"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HAY9158397.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISAGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGDVHAFADEVKELGQSLPRFTAHTWHRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            16533..17786
                     /locus_tag="JNC24_001632"
     CDS             16533..17786
                     /locus_tag="JNC24_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HAY9158398.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARFPVYA"
     gene            complement(17984..19177)
                     /locus_tag="JNC24_001633"
                     /pseudo
     CDS             complement(17984..19177)
                     /locus_tag="JNC24_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311443.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
     gene            19295..22572
                     /locus_tag="JNC24_001634"
                     /pseudo
     CDS             19295..22572
                     /locus_tag="JNC24_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417044.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
     gene            22669..23652
                     /locus_tag="JNC24_001635"
     CDS             22669..23652
                     /locus_tag="JNC24_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAY9158399.1"
                     /translation="MPKKMRTTRNLLLMATLLGSALFARAADKEMTIGAIYLDTLGYY
                     AGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSS
                     RTVRRASEAGIPVICYNTCINQKGVDKYISAYLVGDPLEFGKKLGNAAADYFIANKID
                     QPKIAVINCEAFEVCVQRRKGFEEVLKSRVPGAQIVANQEGTVLNKAISVGEKLIIST
                     PDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDIS
                     GKKMGNAVFAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
     gene            23675..25186
                     /locus_tag="JNC24_001636"
     CDS             23675..25186
                     /locus_tag="JNC24_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HAY9158400.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASC
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKITRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISANGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            25211..26209
                     /locus_tag="JNC24_001637"
     CDS             25211..26209
                     /locus_tag="JNC24_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY9158401.1"
                     /translation="MSASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            26242..27336
                     /locus_tag="JNC24_001638"
     CDS             26242..27336
                     /locus_tag="JNC24_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HAY9158402.1"
                     /translation="MKINRIVNEGFMKTMLAAYLPGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGVMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYALMASGKCGKVVINFPD"
     gene            27348..28220
                     /locus_tag="JNC24_001639"
     CDS             27348..28220
                     /locus_tag="JNC24_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HAY9158403.1"
                     /translation="MTIYTLSHGSLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(28268..>28678)
                     /locus_tag="JNC24_001640"
     CDS             complement(28268..>28678)
                     /locus_tag="JNC24_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HAY9158404.1"
                     /translation="RYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQYMAS
                     LGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDMTGDA
                     VGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
BASE COUNT         6818 a         7092 c         7790 g         6978 t
ORIGIN      
        1 aatgcccggg aattctcctc ggtggaaaaa gaagtccatt gtgtagaaac ctcccgacga
       61 tctgcttcag gaaaccaggc tgccacagtt tcgggaagag gggggatctg aaagttctgc
      121 cccccttcag tgatgactct gatatggcgc gaatgtaaat tttgcggcaa catctggcgt
      181 aaaaacagtc tgttttggtc tgacagggaa atacgaaact gaggcccgtt aaaatttgga
      241 accgaagttt gctgtattaa gaatagcaga gtttgatctg ataaggggtt atttgcaatc
      301 tcaatagtta tatgaggatc taaacgaaaa agatttgtcg ggattgtagt tatttggttg
      361 ttttcaaaat aaaaaaatct tctcatgata atattttctt ggatgtttgg taagttgctt
      421 aatctattgc tatatgcaaa aagaagttgc agtctcctag gtaaaggggg cagttcagta
      481 agatggttgt gcgcaacctt aagtatctta agattagaag gcagagatgg aagagtttgc
      541 agttcgttgt tcctaacctc aagttttttt aaattcaagg gtaatgaagg cagtgtggat
      601 aacctattat actctgcgct tagtgatttg atggtgtctg gtaataacgg cagattctct
      661 aattgatttt cggacacatc caaagttatt agataatgtg gtaatgcaga tatatgtgtt
      721 aagaaattat ctcttgcaat aagttcttta atattttcag gtaaatcagt aaggatggtt
      781 aatttattac agctcacatc aagcttttta atgcaagcag ggagtacagg taacgaggaa
      841 agcgccaagg ctcgcaggtc cagtttatgc cccttagagg taagacatgc acgaagtctt
      901 cttaccgcct cagtacgctg ttcttccggt aacccctgtt tctcccaatc atcccattcg
      961 gacaaataat ggttggggga tgtaattgtg ctgatatgtg gaaataaaca ggatgaaata
     1021 tttctgagca tattaatttc tcgcatatgg atatggaatt agattgtgaa taaattgtat
     1081 ttaatgtatc tctcctaatg aggagggtga ttcagaatat tcgccataag ggaatgagtg
     1141 ttatatcatg ctttctaccc tggtaagtat atttgctttt ttcgtatatt tggttcatct
     1201 ttttttgatg ttttacatat tcacaatgca gaggatttat accagaagac attagtcttg
     1261 agtaagatac tacaacatct ccaactattt tagccagcct gactacttga cttggtaata
     1321 tttgttgcta ataatcatgt ataaaataag cttatataca tagtaacttc agcaaaggat
     1381 ttagtaatga tccaaatcat atctcaatgt actgtttttt tctttaaaaa aacagtacat
     1441 tctactttat aatgactgtg tattcccatg gaaggagatc acccaattct cccccccgac
     1501 ttttgacgca gtgtcttgtt aacaggggga tttaccatgc ttattggcta tgtacgcgta
     1561 tcaacaaatg accagaatac cgatctacaa cgtaatgcgt tgaactgtgc aggatgcgag
     1621 ctgatttttg aagacaagat aagtggcaca aagtccgaaa ggccggggct gaaaaaactg
     1681 ctcaggacat tatcggcagg cgacacactg gttgtctgga agctggatcg gctggggcgc
     1741 agtatgcggc atcttgtcgt gctggtggag gagttgcgcg aacgaggcat caactttcgt
     1801 agtctgactg attcaattga taccagcaca ccaatgggac gctttttctt ccatgtgatg
     1861 ggtgccctgg ctgaaatgga acgtgaactg attgttgaac gaacaaaagc aggactggaa
     1921 gctgctcgcg catagggacg aattggcggt cgccgtccca aactttcacc agaataatgg
     1981 gcgcaggccg ggcgactaat tgcatcagga gtttctcgcc agaaggtggc gatcatctat
     2041 gatgttggcg aatcgacttt tttgtataag aggtttccag ccagggttat ataaaaccgc
     2101 agacacggcg tatacaagaa cgtgctacgg ttgactgaaa agttaatgtg gtatgtcaca
     2161 ggccttttgc gccgtaaagc cccgtccttt agggcgcgga ggacgttaaa gaaagtggtg
     2221 ttctttgtgt tgctgaactt atgcaaaatt tttttgcacc ataaatccaa caacacattc
     2281 aataacacat tgctgtatca ctatgaaaat aaatgtgttt ttatttgatt tcacacatat
     2341 cacaattgat gcatttttta gtgattaaca acgccataag gaaactctca aaacaacaca
     2401 aaccgggcgg gcattgtacg cgcattcttt actcagacca gttttttcac cagcgcttca
     2461 ctatgacgaa tacgttctgg tgccagttcc agatcctgct gaatcagcgc aatgaacagc
     2521 aaatccgtta acattccctg agcgtgacaa gcagagattg aagcactgtt tgtcgcttgt
     2581 tcttcggcaa tggtatacag gcaatgagaa gcacgctgtt gcagggcatt cggagtaaag
     2641 ccggtaatcg ccagcacttt tccgcccact cgcagcatct catctgccgc caggtttaac
     2701 tcgcgtcgta caccggtgta ggaaatggct aataacagat cgtcagggga cgacgcctgt
     2761 actgttgcga gtagcgcatg catatcgcgc actgcggcag cattgaagcc aatcttcatc
     2821 agcttccagg caaagttttg cgccaccaga cccgaagcgc caataccggt cagaattatc
     2881 cgccgcgcag agcgcaacat tgttacgcat tcatgcagtt tctcttcact attaacgttt
     2941 agcgttgcgt acatagcggc agtattttct ttaatcagtt tttcgccgac cagccgtaac
     3001 ggatcatcac cgcggatttg gttatgaatg ggcacggagg gcgattccgg ctggcttgcc
     3061 agcgcttcac tcaacgccaa cttaagcgcc ggaaaacctt tatagccgag tttttgcgcg
     3121 aacttcacga cgctggactg actgactccg gcttcgttgg ccagttgctg agagcttaaa
     3181 tggcgcgccg tatcaggttg tagcagcaga taatccgcca gttttttatc actctgggca
     3241 agcccctggt aacgctgacg gatacgaagt aagccgttca tatctttcct cgcgatgaaa
     3301 tagtggcgac aacaggcaat atgagacaca cgcgagtgaa ttttatattc cattgccgct
     3361 aattgccatg aattaaaaat tccagttagc catgatgcgg tcgattgttg ccggaaaagt
     3421 gatgatctat ctgttaggct atgtagtagc gaaaattaat caggatattt cagtccagag
     3481 gagtatggtt tggcaactca cgagcgtcgt gtggtgtttt ttgacttaga tggaacattg
     3541 catcagcagg atatgttcgg cagttttctg cgctatttac tgcgtcgcca accgctgaat
     3601 gcgttacttg tcctgccgtt gttaccgatt atagccattg cgttattgat aaaaggtcgt
     3661 gcggcacgct ggccgatgag tctgcttctg tgggggtgca cttttggtca cagcgaagca
     3721 cgtttacaga cgttgcaggc cgatttcgtg cgctggtttc gcgacaatgt taccgccttt
     3781 ccgctggttc aggagcgatt aaccacctac ctgttaagtt ccgatgctga tatctggttg
     3841 attaccggct ctccgcagcc gctggttgaa gcggtttatt tcgatacgcc ctggctgccg
     3901 cgggttaatc ttatcgccag ccaaattcag cgtggctatg gtggttgggt attgacgatg
     3961 cgttgtctgg gacatgaaaa ggtcgcacaa ctggagcgca aaatcggcac tccgctgcgg
     4021 ctgtacagtg gctatagcga cagtaatcag gacaatccgc tgctttattt ctgtcagcat
     4081 cgttggcgag taaccccgcg cggtgaactc cagcaactgg aatagagtaa agcatagcgt
     4141 ccgtgtataa tgcgccgcgc ttttataacc ggagttttct ttttgtctga agtcgaattt
     4201 agccacgaat actggatgcg tcacgcgctg acgctggcga aacgtgcctg ggatgagcgg
     4261 gaagtgccgg tcggcgcggt attagtgcat aacaatcggg taatcggcga aggctggaac
     4321 cgcccgattg gtcgccatga tcccaccgca catgcagaaa tcatggccct gcggcagggt
     4381 ggtctggtga tgcaaaatta tcgtctgatc gacgccacgt tgtatgtcac gcttgaacca
     4441 tgtgtaatgt gtgccggagc gatgatccac agtcgcattg gtcgcgtggt ctttggtgcg
     4501 cgtgacgcga aaactggcgc tgcgggatct ttaatggatg tgctgcatca tccgggtatg
     4561 aatcaccgag tggaaattac ggaaggaata ctggcggatg agtgcgcggc gttgctcagt
     4621 gacttctttc gcatgcgccg ccaggaaatt aaagcgcaga aaaaagcgca accctcgacg
     4681 gattaatttt gtttctcttc actccctttc ctggaaaaca gcagagaggg agaatgggtc
     4741 aaatagtttg atgacgactg ggaaagaaac gagagaaaag gaaatttctc tttgcccaac
     4801 tccgtaggcg ataccgccgg ataatcctgc gccagttgca tcgccgcttc tgtagcctgc
     4861 ttctctttct cttgcagata acccaccagg ctaatctgat acttacgaat attttcgaca
     4921 taagcgtagg cttcatgtcc acgagcgtag ccgtaagtca gcttgctgta atagggtttc
     4981 tggctaagta aaggcagaca ctgttttacg tcagcccaac tgtcaggatt ccctttggtt
     5041 tttgccgtca gggcgcgggc atccagcata tgcgcatagc ccatattgta cgcagcgagg
     5101 gcaaaccaga tccgctcgtt ctccggcaca ctttccggca ctttactcat catatcctgc
     5161 aaataacgca cgccaccgct gatgctctgt tcggcatcgg tacgatccgt aatgccgagg
     5221 ctttgcgcgg tatttttggt taacatcatc atgccgcgca cacccgtcgg tgaagtggcc
     5281 tgtgcatccc agtgcgattc ctgataagca atagcggcca gcaaacgcca gtcaatttct
     5341 tcggcgtatt tctcaaacag gggctttaac tgcggcagta ccgcatcgac ggcgcgtaaa
     5401 aatgtgcgcg tatcgacgta atcaaaatca tcgccatgcc ccaggtattt ctcttcaatg
     5461 cgtgccagcg taccgtcttc attcatttcg ttgaagaagt cgagcagggc ggcggaaagg
     5521 gtattatcgc catctaacgg gctaaaccaa gtcaccggtt gttcatcggt gatatcgagc
     5581 gctacggcga gctccgggtg aacgcgctga aacaggctga tggcgacaga atcagcaatg
     5641 gtgtaatcga gttttccttc gatgacatct tccattaatt ccgcagagcc ttttttgtcg
     5701 tctaccttcc agcttaattc cgggaatttt gtgtctttca gggtttggag atcgttaacc
     5761 accacgtgac ccggtgcaac ggtgagttgc tccgccgtca ggttgcccag tgtgcgcggg
     5821 cgatactgac tcactttata aaccagttgt tgtgacacgg aataataggt agggccaggc
     5881 tgataatttt ttacccgctc actgttatag accagtcctg ccgccagcag gtcggcgtta
     5941 ccattatcga ggtcgtcaaa cagctggctg atattctgcc gcacggtcac tttcagtttt
     6001 acaccgaggt aatcggcaaa ctgtttcgcc agttcgtaat ccaggccaaa aggtttcccg
     6061 ttgatttcgt tataagtcag gggagtatga atggtgctca cacgcaactc tccccgcgct
     6121 tgaatggcgg cgatacggtt gtcggcttta ccaaaccagg gaatggatgg ccagagagcg
     6181 accgcgagca gcagtgccag aatgccgatg aacagataat taatctttaa ttttttcaat
     6241 tagttaattc tctgtgtcgt gcgcgtccca gcttgaaaaa acgtaataat agtgaaaggt
     6301 ttactcataa atgagcggca ttttgcgtaa acctgcgcca gatggcaact tattacagcc
     6361 attggcggca cgcgttgcta attcacgatg gtgattttat ttccacgcaa acggtttcgt
     6421 cagcgcatca gattctttat aatgacgccc gtttcccccc cttgggtaca ccgaaagctt
     6481 agaagacgag agacttatga tggaaattct gcgtggttcg cctgcactgt cggcattccg
     6541 aatcaacaaa ctgctggcac gttttcaggc tgccaggctc ccggttcaca atatttacgc
     6601 cgagtatgtc cattttgctg acctcaatgc gccgttaaac gatgatgagc acgcacaact
     6661 tgaacgcctg ctgaaatatg gcccggcact cgccagccac gccccgcaag gcaaactcct
     6721 gctggtgacc ccgcgtcctg gcaccatctc tccctggtct tcgaaagcga ccgatattgc
     6781 ccataactgc gggctacaac aggtaaaccg ccttgagcgc ggcgttgctt actatataga
     6841 agccggtacg ctgaccaatg aacaatggca gcaggttacc gctgaactgc acgaccgcat
     6901 gatggaaacg gtcttttttg ctttagatga tgcagagcag ttgtttgccc accatcaacc
     6961 gactccggtt accagcgttg atttgctggg gcagggccgt caggcgctga tcgacgctaa
     7021 cctgcgtctt ggcctggctc tggcggaaga tgaaattgac tatctgcagg atgctttcac
     7081 aaagcttggt cgtaacccga acgacatcga gctgtatatg ttcgctcagg cgaactctga
     7141 gcactgccgc cacaaaattt ttaatgccga ctggattatc gacggtgaac agcagccgaa
     7201 atcgctgttc aagatgatca aaaatacttt cgaaaccacg ccagatcacg ttctctctgc
     7261 ttataaagat aacgccgccg taatggaagg ttctgaagtg ggccgctact ttgctgacca
     7321 cgaaacgggc cgctacgatt tccatcagga accggcgcat attctgatga aagtcgaaac
     7381 tcacaaccac ccgacggcga tttctccgtg gccgggcgcg gcgacgggtt ccggcggcga
     7441 aattcgtgat gaaggcgcca ccggacgcgg tgcgaagccg aaggctggtc tggttggttt
     7501 ctccgtttcc aacctgcgaa ttcctggctt cgaacagccg tgggaagaag atttcggtaa
     7561 gcctgagcgc attgtcaccg cgctggacat catgaccgaa ggcccgctgg gcggcgcggc
     7621 gtttaacaac gaatttggtc gtccggcact gaatggctac ttccgtactt atgaagaaaa
     7681 agtgaacagc cacaacggcg aagagctgcg cggttatcac aaaccgatca tgctggcggg
     7741 cgggatcggc aacattcgcg ccgatcacgt acaaaaaggc gagatcaacg tcggtgcgaa
     7801 gctggtcgtt ctcggcggcc cggcaatgaa catcggtctt ggcggcggag cggcgtcttc
     7861 catggcgtca ggtcagtctg atgccgacct cgactttgct tccgtacagc gcgacaaccc
     7921 ggagatggag cgtcgctgcc aggaagtgat cgaccgttgc tggcagcttg gtgatgccaa
     7981 cccaatcctg tttatccacg acgtcggcgc tggcggtctt tctaacgcga tgccggaact
     8041 ggtgagcgac ggcgggcgcg gcggtaaatt tgaactgcgc gatattctaa gcgacgaacc
     8101 gggcatgagc ccgctggaaa tctggtgtaa cgaatcccag gaacgctacg tgctggcggt
     8161 tgctgccgat caattaccgc tgtttgacga actgtgtaag cgtgagcgcg caccctacgc
     8221 ggtgattggt gaagcgaccg aagaactgca tctttctctg cacgatcgtc attttgataa
     8281 tcagccgatc gatctgccgc tggacgtcct gcttggtaaa acgccgaaga tgacccgcga
     8341 tgtacaaacg ctgaaagcga agggcgatgc gctggtccgt gaagggatca ccattgcaga
     8401 cgcggtgaaa cgcgtactgc atctgccgac cgtggcagag aaaaccttcc tggtgaccat
     8461 cggcgaccgc agcgtaaccg gcatggtagc gcgcgatcag atggtggggc cgtggcaggt
     8521 gccggtcgct aactgcgcgg tcactaccgc cagcctcgac agctactacg gtgaagcgat
     8581 ggcgattggc gagcgtgcgc cggttgcact gctggatttc gccgcctctg cccgtctggc
     8641 ggtcggtgaa gcgttaacca atattgccgc aacacagatt ggcgatatca aacgcattaa
     8701 actttccgcc aactggatgg cggcggcagg ccaccctggt gaagatgcgg gcctgtatga
     8761 agccgttaaa gccgtgggcg aagagctttg tccggcgctg ggcctgacga tcccggtggg
     8821 taaagactct atgtcgatga aaacccgctg gcaggaaggt aacgaagagc gcgaaatgac
     8881 gtcgccgctg tcgctggtga tttctgcatt tgcccgcgtg gaagatgtgc gtcacaccat
     8941 cacgccacag ctttctaccg aagataacac actgctgctg attgatttgg gcaaaggcaa
     9001 taacgcgctg ggcgcaacgg cgctggcgca ggtttatcgt cagcttggcg acaaaccggc
     9061 agatgtacgc gacgtcgcgc aactgaaagg cttctatgac gcgattcagg cgctggttgc
     9121 acagcgtaag ctgctcgcat accacgaccg ctctgatggc ggcctgctgg taacgctggc
     9181 ggaaatggcc tttgctggtc attgcggcat taacgcggat attgcctctc tgggcgacga
     9241 tcgcctggcg gcgttgttta acgaagaact gggtgcggtg attcaggttc gtgccgctga
     9301 ccgtgaagcg gtcgagtccg tactggcaca gcatgggctt gctgattgtg tccattatgt
     9361 agggcaggcg gtttccggtg accgttttgt gattaccgcc aacgggcaga ctgtattcag
     9421 cgaaagccgc accacgttgc gtgtctggtg ggcagaaact acctggcaga tgcagcgcct
     9481 gcgtgacaac ccggagtgtg ctgaccagga gcaccaggcg aaatcaaacg acgccgatcc
     9541 gggcctgaat gtgaaactgt cgttcgatat caacgaagat gtggcagcac cgtatattgc
     9601 cactggcgca cgtccgaaag ttgctgtgct gcgtgagcag ggcgtgaact cgcatgttga
     9661 aatggcggca gctttccacc gtgcaggctt tgatgctatc gacgtgcata tgagtgacct
     9721 gctgaccgga cgcacgggcc tggaagattt ccacgccctg gtcgcgtgcg gtggtttctc
     9781 ctacggtgat gtgctgggtg ccggtgaagg ttgggcgaag tcaatcctgt tcaatgaccg
     9841 tgtacgcgat gagtttgcaa ccttcttcca ccgtccgcaa acgctggcgc tgggggtatg
     9901 taacggttgc cagatgatgt ctaacctgcg tgaactgatc ccggggagcg agttgtggcc
     9961 acgttttgtg cgtaacacct ccgatcgctt tgaagcgcgt ttcagcctgg ttgaagtaac
    10021 ccaaagcccg tctctgctgt tgcaggggat ggtgggctcg caaatgccga ttgctgtctc
    10081 tcatggtgaa gggcgcgtgg aagtgcgtga tgcggcgcat ctggcggcac tggaaagcaa
    10141 agggctggtg gcactgcgct atgtcgataa cttcggcaaa gtcactgaaa cctacccggc
    10201 taacccgaac ggttcaccga acggtattac ggcggttacg actgaaagtg gtcgagtcac
    10261 cattatgatg ccgcacccgg aacgtgtttt ccgtactgtc agcaactcct ggcatccgga
    10321 aaactggggc gaggatggcc catggatgcg cattttccgc aatgcgcgta agcagttggg
    10381 gtaagtcatc agttcattgg ttttgcagcc cggatgcggc attaagcacc aaccactatc
    10441 ataagctccc aactccggga gcttttttgt gtctgtaaat cacgacaatg ggtggtttac
    10501 cgtgtcgctt tctggcgaca cttaaatgat tgattttatt gcaatctaat aagtttatct
    10561 taagttgtct ctttttagcg acacagtggc tgatttacat tcaaatagcg aagaagccat
    10621 aaatccctca aagcaacgta taatcagtac gttaccaaac tattttcttt attggcacag
    10681 ttactgcata atagtaagca gtggctcatt caccgactta tgtcagcccc ttcgggacgt
    10741 gctacataaa attcgaatga cgcacaacaa ggtgcctgcc gtccaacttc tgatatcagc
    10801 gtagctatat caaccatcgg gcgaaacgtc gagttaggca ccgccttatt ccataacaaa
    10861 gccgggtaat tcccggcttt gttgtatctg aacttcccct cggttagcat caggctattc
    10921 gcgtctgacg agagtaacac cttgaaacgc tggcccgttt ttccccgctc attacgacaa
    10981 ctggtaatgc tggcattttt gctaattcta ctacccctgt tggtgctggc atggcaagcc
    11041 tggcaaagcc tgaatgcgct tagcgatcag gcggcgctgg ttaaccgcac tacgcttatt
    11101 gatgcccggc gtagtgaagc gatgaccaac gcggcgctgg agatggagcg tagctaccgt
    11161 cagtattgcg tgctggacga ccctacgctt gcgaaggttt atcaaagcca gcgcaagcgt
    11221 tacagcgaaa tgctcgatgc ccacgcaggc gtactgccag acgataaact ttaccaggca
    11281 ttgcgtcagg atttgaacaa tctggcccaa cttcagtgta acaacagtgg tcccgatgct
    11341 gccgccgccg cgcgtcttga agcctttgcc agtgccaata ccgaaatggt gcaggccacg
    11401 cgcacagtgg tgttctctcg tgggcagcaa cttcagcgtg aaatcgccga acgtgggcaa
    11461 tattttggtt ggcaatcgct ggtgctattt ctggtgagtc tggtgatggt gctacttttc
    11521 acgcggatga ttatcgggcc ggtgaaaaat atcgagcgca tgatcaaccg tctgggggaa
    11581 gggcgttctc tgggcaatag cgtttcgttc agtggaccga gcgagttacg ctcggttggg
    11641 caacgtattc tttggttaag tgagcgcctg tcatggctgg aatcccaacg ccatcaattt
    11701 ttaagacatt tatctcatga attaaaaacg ccactggcga gtatgcgcga gggcactgaa
    11761 ttactggctg accaggttgt cgggccgctt acgccagagc aaaaagaggt ggtgagcatt
    11821 cttgatagca gcagccgcaa tttgcaaaaa ctgatcgaac aactgcttga ttacaaccgt
    11881 aaacaggcgg acagtgcggt ggaactggag aatgttgaat tagcaccgct ggtggagaca
    11941 gtggtttctg ctcatagcct gcccgcacgg gctaaaatga tgcataccga cgtcgatctc
    12001 aaagcaacag cttgcctggc ggagccaatg ctgctgatga gcgtactgga taatctttac
    12061 tccaatgcgg tgcactacgg ggctgaatcc ggtaacattt gccttcgcag cagttcacat
    12121 ggtgcgcggg tttatattga tgtcatcaat acaggcacgc ccattccgca agaggaacgc
    12181 gccatgatct tcgaaccctt ttttcaggga agccaccagc gaaaaggggc ggtgaagggc
    12241 agcggtctgg gattaagcat tgccagggat tgtattcgcc gtatgcaagg ggaactgtat
    12301 ctggtcgacg agagcgggca agacgtttgt ttccgcattg aattaccgtc gtcgaaaaac
    12361 acgaaataat gctaacagcc tatcctatca ggctatttta tttgtcagtt cggccccggg
    12421 cagtgctcga aatcttcacg tacgatatgt acgctccggt ttctccgcgc tgtccatgtc
    12481 cagactgtct gcaacaatta cgcccactgg gataggcttt aagtctggtg aatatgcgac
    12541 acatttttca acgattattg ccccgacggc tgtggctggc agggctgcca tgtctggcat
    12601 tgctgggttg cgtgcagaat cacaataagc cagccattga tacgccagca gaagaaaaaa
    12661 ttccggttta tcaactggct gactatcttt ctactgaatg tagcgacatc tgggcgctgc
    12721 aaggtaaatc aaccgaaacc aatccgcttt actggctgcg ggcgatggat tgtgctgatc
    12781 gtttaatgcc tgcgcagtcg cgtcagcaag ctcgccaata tgacgacggc agctggcaaa
    12841 atacctttaa acaagggatt ttgctcgccg acgccaaaat tacgccgtat gagcgccgcc
    12901 agctggtcgc gcgtattgaa gcgttaagta ccgagatccc ggcacaggtt cgcccactgt
    12961 atcaactctg gcgcgacggt caggcgctgc aactgcaact ggcggaagag cgccaacgtt
    13021 acagtaaact ccagcagtcc agcgacagcg agctggatac attgcgccag caacatcacg
    13081 ttctacagca gcaactggaa ctcaccaccc gcaagctgga aaacctgacc gatattgaac
    13141 gccagctctc gacccgcaaa ccgactggaa atttctcgcc ggatacgccg cacgagagtg
    13201 aaaagcccgc gccatccacc catgaggtca ctcctgatga gccataaacc tgcgcattta
    13261 ttattggtcg atgacgatcc gggattgctg aaactgcttg gcctgcgcct gaccagcgaa
    13321 ggctacagtg tggtcacggc ggaaagtggc gctgaaggat tacgggtact gaatcgcgaa
    13381 aaagtagatt tagttatcag cgacctgcgg atggatgaaa tggacggtat gcagctattt
    13441 gctgaaatcc agaaagtgca gccgggaatg ccagtaatta ttcttactgc gcatggttct
    13501 atccccgatg ccgttgctgc aacacagcag ggcgttttta gtttcctcac caagcctgtc
    13561 gataaagatg cgctatatca ggcaattgac gatgcgctgg agcaatccgc gccagccacc
    13621 gatgaacgct ggcgcgaggc aattgtcacc cgcagcccgc tgatgctgcg tttgctggaa
    13681 caggcgcggc tggtggcgca atcagacgtc agcgttttga ttaacggtca gagcggcacc
    13741 gggaaagaga ttttcgccca ggctatccac aacgccagcc cgcgcaacag caaaccattt
    13801 attgctatta actgtggcgc attgcccgag caattgctgg agtcggagct gtttggtcat
    13861 gcgcgtggcg cgtttactgg cgctgtcagc aatcgcgaag gtttattcca ggcggcggaa
    13921 ggcggaacgc tatttctcga tgaaattggc gatatgcctg cgccgttaca ggtcaaactg
    13981 ctgcgcgtgt tgcaggagcg taaagtgcgc ccgctgggca gtaaccgcga tattgatatc
    14041 aatgtgcgga ttatttctgc cactcaccgt gatctgccaa aagcgatggc gcgcggggaa
    14101 ttccgcgaag atctctatta ccgcctcaac gttgtcagcc tgaaaattcc ggcactggcg
    14161 gagcgcacag aagacattcc gctactggca aatcacctgt tgcgccaggc ggcagagcga
    14221 cataaaccgt ttgtccgcgc gttctctacc gatgcgatga aacgcctgat gaccgcgagc
    14281 tggccgggta atgtgcgcca gttggtcaac gtgattgaac agtgcgtggc gctgacctca
    14341 tctccggtga ttagtgatgc gctggtggag caggcgctgg agggtgaaaa tacggcgctg
    14401 ccaacctttg ttgaggcacg taatcagttt gaactcaact atttgcgtaa gctgctgcaa
    14461 atcaccaaag gcaacgtcac ccacgcggcg agaatggcgg ggcgcaaccg gacagaattt
    14521 tataaactgc tttcccgaca cgagctggat gcaaacgatt tcaaggaatg aattggcgtt
    14581 atgtgttacg tttagcagat caaaagacag gcgacctttt caaggaatag catgaaaaag
    14641 attgatgcga ttataaaacc cttcaagctg gacgatgtcc gcgaagcact ggccgaagtc
    14701 ggtattaccg gcatgacggt gaccgaagtg aaaggctttg gtcgccagaa aggccatacc
    14761 gagctgtacc gcggcgcgga gtatatggtg gattttctgc cgaaagtgaa aattgagatt
    14821 gtcgtaccgg acgacattgt cgatacctgt gtcgatacca ttattcgcac ggcgcaaacc
    14881 ggcaaaatcg gtgacggtaa aatcttcgtc tttgacgtgg cacgggtcat tcgcatccgt
    14941 accggtgaag aggacgacgc ggcaatttaa accgcgtcaa gggttgccgg atgtttccat
    15001 ccggcaacat caaattacag caccttatgc gggccaaagc attcgtaatg aatgttttcc
    15061 tgcttcacgc ccagatccac taactgtttc gcggtaaact gcatgaagcc aaccgggccg
    15121 catagataga actgcattgt cggatcgctg aacgcacctt ccagtttgct caaatccatc
    15181 agaccttcgc tatcaaactg acctttagcg cgatcggctt cgctcggctg acgatgccag
    15241 gtgtgcgcgg taaagcgcgg cagtgactgc cccagttcct taacttcatc ggcaaaggcg
    15301 tgaacatcgc cattttctgc cgcatggaac cagttcactt gtgctgtgtg gcctgctttt
    15361 gccagcgtgt cgagcattgc cagcattggc gtttgaccaa caccggcaga gattaacgtc
    15421 actggtgtgt catctgcgac agccataaag aaatcacctg ccggagcgac cagtttcacg
    15481 acatcgccaa cattggcgtg attgtgcaac cagttggata cctgcccacc ctcttcgcgt
    15541 ttcaccgcaa tacgatagcc tttgccatcc ggtttgcgag tcaaagagta ctgacgaatt
    15601 tcctgatgcg ggaaaccttc cggcttcagc cagacgccga gatattgccc cggacggtat
    15661 tctgccactg cgccaccgtc gaccggctcc agttcgaagc tggtgataag cgcgctgcgc
    15721 ggtgttttag ccacaatgcg gaaatcgcga gtaccttccc aaccaccggc tttgctggcg
    15781 ttttcgttat agatttccgc ctcgcgattg ataaatacat tagccagtac accataggct
    15841 ttaccccacg cgtccagcac ttcctgcccc gggctgaaca tttcgtccag cgttgccaac
    15901 aggtgttcac cgacgatgtt gtactgttcc ggtttgatct ggaagctggt gtgcttctgc
    15961 gcgatttttt ctaccgctgg cagcagcgca ggcaggtttt caatattact ggcgtaggcg
    16021 gcaatagcgt taaacagggc ttcacgttga tcgccattac gctggttact catgttaaaa
    16081 atttctttga gttctgggtt atgagtaaac atacggtcgt agaaatgggc ggttaacttt
    16141 ggccccgttt ccaccagtaa agggatggtg gcttttactg tagcgatggt ttgagcgtca
    16201 agcatatggt cttccttttt ttgcatctta attgatgtat ctcaaatgca tcttataaaa
    16261 aatagccctg caatgtaaat ggttctttgg tgtttttcag aaagaatgtg atgaagtgaa
    16321 aaatttgcat cacaaacctg aaaagaaatc cgtttccggt tgcaagctct ttattctcca
    16381 aagccttgcg tagcctgaag gtaatcgttt gcgtaaattc ctttgtcaag acctgttatc
    16441 gcacaatgat tcggttatac tgttcgccgt tgtccaacag gaccgcctat aaaggccaaa
    16501 aattttattg ttagctgagt caggagatgc ggatgttaaa gcgtgaaatg aacattgccg
    16561 attatgatgc cgaactgtgg caggctatgg agcaggaaaa agtacgtcag gaagagcaca
    16621 tcgaactgat cgcctccgaa aactacacca gcccgcgcgt aatgcaggcg cagggttctc
    16681 agctgaccaa caaatatgct gaaggttatc cgggcaaacg ctactacggc ggttgcgagt
    16741 atgttgatat cgttgaacaa ctggcgatcg atcgtgcgaa agaactgttc ggcgctgact
    16801 acgctaacgt ccagccgcac tccggctccc aggctaactt tgcggtctac accgcgctgc
    16861 tggaaccagg tgataccgtt ctgggtatga acctggcgca tggcggtcac ctgactcacg
    16921 gttctccggt taacttctcc ggtaaactgt acaacatcgt tccttacggt atcgatgcta
    16981 ccggtcatat cgactacgcc gatctggaaa aacaagccaa agaacacaag ccgaaaatga
    17041 ttatcggtgg tttctctgca tattccggcg tggttgactg ggcgaaaatg cgtgaaatcg
    17101 ctgacagcat cggtgcttac ctgttcgttg atatggcgca cgttgcgggc ctggttgctg
    17161 ctggcgtcta cccgaacccg gttcctcatg ctcacgttgt tactaccacc actcacaaaa
    17221 ccctggcggg tccgcgcggc ggcctgatcc tggcgaaagg tggtagcgaa gagctgtaca
    17281 aaaaactgaa ctctgccgtt ttccctggtg gtcagggcgg tccgttgatg cacgtaatcg
    17341 ccggtaaagc ggttgctctg aaagaagcga tggagcctga gttcaaaact taccagcagc
    17401 aggtcgcgaa aaacgctaaa gcgatggtag aagtgttcct cgagcgcggc tacaaagtgg
    17461 tttccggcgg cactgataac cacctgttcc tggttgatct ggttgataaa aacctgaccg
    17521 gtaaagaagc agacgccgct ctgggccgtg ctaacatcac cgtcaacaaa aacagcgtac
    17581 cgaacgatcc gaagagcccg tttgtgacct ccggtattcg cgtgggtact ccggcgatta
    17641 cgcgtcgcgg cttcaaagaa gcagaagcga aagaactggc tggctggatg tgtgacgtgc
    17701 tggacagcat caatgatgaa gccgttatcg agcgcatcaa aggtaaagtt ctcgacatct
    17761 gcgcacgttt cccggtttac gcataagcga aacggtgatt tgctgtcaat gtgctcgttg
    17821 ttcatgccgg atgcggcgtg aacgccttat ccggcctaca aaactttgca aattcaatat
    17881 attgcaatat ccgtgtaggc ctgataagcg tagcgcatca ggcaatctta ggtatgtgat
    17941 catcaaggct tccttcggga agcctttcta cgttattgcg ccatcaaatc tgtcgtaact
    18001 gcgcctcaac atacaaatag ccaatcccca acacctgttg tgcgcggctt aattgcccaa
    18061 agccaatttg cgttgctttc acgctcggcc cttcgtcgcg gtcgaacggg ttaaatcccg
    18121 tctggcgaat aatggtatta agccagtgct caccaaaggc acaactgcga ccgtacaacc
    18181 aaatctgatt aatattgagg atgttgagga agttatacag actcaaacca atggcattgg
    18241 cactgcgatc aacccaactg gtgatccacg gttctccact ctaccaggca gcgattaact
    18301 gcgctgtagt cagtttttcc ggatcaagct gagtattaac cggttgtgat tttagccata
    18361 cccgcgcctg tttttttaat gcacttaacg aggcgacggt ttccaggcag ccgtaacgtc
    18421 cacagtcgca gacgacgcca tccggattaa cgatggtgtg actaatctgt ccgctaccat
    18481 acaaactgcc gcgataaatt tgctcgttaa tcacgaacga cgagccaatg ccgtaatcaa
    18541 cgttgatcac gcagaaatcc cgtttctgcg aattattttg ccatttctcc gccagcgcca
    18601 gcatcacgca gtcattatcg accatcaccc gaatgccgag cttctcttcc agcagatact
    18661 tcacctcaac cggcgttgtc cacggcgctt gcggcatggt ttgcgacacg ccggtcaccg
    18721 gatcaacctg accgtggatt gccagcgcca gattgatggt gcggtccggc cacaatttac
    18781 ggtggcgatg ccagcatttt tcgatttcgg acagcagcgc ctgcggcgtc ggtgcatcaa
    18841 tctgtaaata ctcaaattca cctttcggac ttaaacaagc attagcaacc tgacactcaa
    18901 tactggtggg cgtcacgttc aggcacagcg tccagtcacc ttccggcgca atcagccatg
    18961 taccgctact atgcccgcgc gtctggcttt catcgtcgat attcaccacc cgtttttcgc
    19021 tttccagttc ctgcaaaata ttactgactg ccggaatcga aatttgcgcc agccgggcca
    19081 gcgttgattt attggcgcgc ttttgccggt acagcagttc cagaatcacg catttgttat
    19141 ggtggcgaat ctgttgattg ttaatgcagg ctctcatctt cactaaacct tgtttagtta
    19201 attgcgtgat tattccgcga aaacattgcc agattgttca atacactgcc acaaatcttt
    19261 taattcagta tgtcttgtta atattgaggg caccatgact ccagtaaaag tgtggcaaga
    19321 gcgcgttgag atcccgacct atgaaaccgg gccgcaggat atacatccca tgttcctgga
    19381 aaatcgcgtt tatcagggat cgtccggcgc ggtttatccc tacggcgtga ccgatacgct
    19441 gagcgagcag aaaaccctga aatcctggca ggcggtgtgg ctggaaaacg actacatcaa
    19501 agtgatgatc ctgccggaac tgggcggtcg ggtgcatcgc gcgtgggata aagtgaaaca
    19561 gcgcgatttt gtttatcaca atgaagtcat taaacctgcg ctggtggggc tgctggggcc
    19621 gtggatttcc ggtgggattg agtttaactg gccgcaacat catcgcccga ccacctttat
    19681 gcccgttgat ttcaccctcg aagcccatga agacggtgca cagacggtgt gggtaggcga
    19741 aacggagccg atgcatggtt tacaggtgat gacaggtttc accctgcgcc ctgggcggcg
    19801 ctggaaatcg ccagccgcgt ctataacggc aacgccacgc cgcgtcattt cttgtggtgg
    19861 gccaacccgg cagtgaaagg gggtgaaggg catcagagcg ttttcccacc ggatttgact
    19921 gcggtgtttg atcacggcaa acgggccgtc tccgctttcc ccatcgctac cggcacttac
    19981 tacaaagtgg actactccgc tggagtggac atttctcgct ataaaaatgt gcccgttcca
    20041 acctcatata tggccgaaaa atcgcagtac gattttgtcg gcgcgtggtg tcacgatgaa
    20101 gatggcggtt tgctgcacgt tgccaaccac catattgcgc caggtaaaaa acagtggagt
    20161 tggggacaca gtgaatttgg ccaggcgtgg gataagagtc tgaccgacaa taacggcccg
    20221 tatatcgaac tgatgaccgg tatttttgcc gataaccagc ccgattttac ctggcttgat
    20281 gcttacgaag agaagcgttt tgagcagtat ttcctgcctt atcattctct gggcatggtg
    20341 caaaatgcct cccgcgatgc ggtgataaaa ctccagcgta gtgagcaggg gattgagtgg
    20401 gggctgtatg ccatctctcc gttgaacgga taccgcctgg cgatccacga aatcggcaaa
    20461 tgcaacgcgt tacttgatga tgccgtggca ctgatgcctg cgaccgccat ccagggcgtg
    20521 ttgcacggta tcaatcctga taggctgacc attgagcttt ccgatgccga cggcaatatt
    20581 gtactgagtt atcaggaaca tcagccgcaa gcgttgccgt taccggacgt cgccaaagcg
    20641 ccactggcag cacaagacat taccagtaca gatgaagcct ggtttatcgg tcagcatctg
    20701 gagcaatatc atcacgccag ccgttcaccg ttcgattact acctgcgcgg cgtggcgctg
    20761 gacccgctgg attatcgctg taacctggcg ctggcgatgc tggaatataa ccgcgcagat
    20821 ttcccgcaag cggtggcgta tgccagtcag gcgctgaaac gcgcacatgc gctgaacaaa
    20881 aacccgcagt gtggacaagc aagtttgatt cgcgccagtg cttacgaacg tcagggacaa
    20941 tatcaacaag ccgaagagga tttctggcgt gcggtctgga gcggcaacag caaagccggt
    21001 ggctattatg gcctggcacg actggcggcg cgtaatggta acttcgacgc tggtctggat
    21061 ttttgccaac aaagtcttcg cgcctgccca accaatcagg aagtgctttg cctgcataat
    21121 ctgctgctgg tgttaagtgg tcgtcaggac aacgcgcgtt tgcagcgcgc gaaactgctg
    21181 cgtgattatc cgctgaacgc cattctgtgg tggctgaact ggttcgatgg tcgtagcgaa
    21241 tcagccctcg cgcagtggcg cggtctgtgt cagggacgcg acgttaacgc cctgatgacc
    21301 gccgggcaac tgattaactg gggaatgccc actctggcga cagagatgct gaacgcactg
    21361 gactgtcagc gcacgttatc gctttatcta caagccagct tgctgccgaa agccgaacgc
    21421 ggcgaactgg tcgcaaaagc cattgatgtc ttcccgcagt ttgtccgttt cccgaatacg
    21481 ctggaagaag tggcggcgct ggagagtatt gaagagtgct ggtttgcccg tcatttactg
    21541 gcctgtttct actacaacaa gcgcagctac aacaaagcca ttactttatg gcaacgttgc
    21601 gtagagatgt cgccggagtt tgccgatggc tggcgcgggt tggcgatcca tgcgtggaat
    21661 aagcaacacg attatgaact ggccgcgcgt tatcttgata atgcttatca gcttgcgccg
    21721 caggatgcac gtctgctttt cgaacgggat ttgctggata agttaagtgg agccacaccg
    21781 gagaaacgac tggcgcgtct ggaaaataat ctggaaattg cgctgaaacg cgacgacatg
    21841 accgcagaac tgctcaattt gtggcatctc acgggtcagg cagacaaagc ggcggacatt
    21901 ctcgccacgc gcaaattcca cccgtgggaa ggcggggaag ggaaggtcac cagtcagttt
    21961 atcctcaacc agttattacg cgcctggcag catcttgatg ccagacagcc gcagcaggcc
    22021 tgcgaactgc ttcatgccgc gctgcattat ccggagaatt taagcgaagg ccgtttaccg
    22081 gggcaaactg ataacgacat ctggttctgg caggcgatat gcgccaacgc gcagggcgat
    22141 gaaactgaag cgacgcgttg tttacgtctg gcggcgaccg gcgatcgcac cattaacatc
    22201 cacagttatt acaacgatca gccggttgat tatctcttct ggcaaggaat ggcgctgcga
    22261 ctgctgggcg aacaacaaat cgcacagcaa ctgtttagtg aaatgaaaca gtgggcgcaa
    22321 gagatggcga aaaccagtat tgaggcggat ttctttgctg tttcacaacc tgacctgttg
    22381 tcgctgtatg gcgatttaca acagcagcat aaagaaaaat gcctgatggt ggcgatgctg
    22441 gcgtccgcgg gactcgggga ggttgcgcaa tatgaatctg ctcgcgctga attgacggcg
    22501 attaatccgg cctggccgaa agcggcatta ttcaccaccg tgatgccttt tatttttaac
    22561 tacgttcact aaaccctatt aactgtaata tttgagcggc acgcgctgcc gctcatcaca
    22621 aaataattag aataagccgt tgttaataaa aactctacag gaggcactat gcctaaaaaa
    22681 atgagaacaa cccgtaatct attattaatg gctacgctat taggtagcgc actatttgcc
    22741 agggctgcgg ataaagaaat gaccattggt gcaatatacc ttgataccct gggatattac
    22801 gctggagtgc gtcagggcgt tcaggatgcg gcaaaagatt cttcagtaca ggtacagtta
    22861 attgaaacta acgcccaggg tgatatttcg aaagaaagta cctttgttga taccctcgtg
    22921 gcgcgtaatg tcgatgccat tattttatcg gcagtgtctg aaaatggcag tagccgtacc
    22981 gttcgtcgcg ccagtgaagc gggtattccg gtgatttgct acaacacctg tattaatcaa
    23041 aagggtgtcg ataaatatat ctcggcgtat ctggtcggcg atccactgga atttggtaaa
    23101 aaactgggta acgctgccgc cgattatttt attgccaata aaattgacca gccgaaaatt
    23161 gccgtcatca attgcgaagc ctttgaagtt tgtgtgcagc gacgtaaagg atttgaagaa
    23221 gtattaaaat cccgcgttcc cggcgcgcaa attgtcgcta atcaggaagg gactgtttta
    23281 aataaagcga tttccgttgg tgaaaaactg attatctcca cgccggatct caacgccatt
    23341 atgggggagt caggcggtgc gacactcggc gcggtaaaag cggtacgtaa tcaaaatcag
    23401 gccggaaaaa ttgctgtttt cggttcggat atgacaaccg aaattgctca ggagctggaa
    23461 aacaatcagg tgctgaaagc ggtagtggat atttccggta agaaaatggg caatgctgtt
    23521 ttcgcgcaaa cattgaaggt tatcaataaa caagccgacg gtgaaaaagt gattcaggtg
    23581 ccaatcgatc tctataccaa aacggaagat ggtaaacagt ggctggcaac gcacgttgat
    23641 ggtctgccct aatcgctgat tcgcagaggt ccctatgttc acggcaacag aggcagtccc
    23701 ggtagcaaaa gtggtggcag gaaataagcg ttaccccggc gtcgttgcgc tggataacgt
    23761 taacttcacg ctcaataaag gcgaagttcg tgcgctgtta ggtaaaaacg gcgcgggcaa
    23821 atcgactctc attcgaatgc ttaccggcag cgaacgcccg gatagcggtg atatctggat
    23881 tggcgagacg cgactggaag gtgacgaagc tacgttgact cgccgtgccg ctgaactggg
    23941 ggttcgtgcg gtttatcagg aattaagtct ggtggaaggg ctgacggtgg cagaaaacct
    24001 ctgccttggt caatggcctc gccgcaacgg catgattgat tacctgcaaa tggcgcagga
    24061 tgcccaacgt tgcttacagg cgctgggcgt tgacgttagt cctgaacaac ttgtttcaac
    24121 gctaagcccg gcgcaaaagc aactggtgga aattgcacgg gtgatgaagg gcgagccgcg
    24181 cgtggtcatt cttgatgaac ccaccagctc gcttgccagt gcagaagttg aactggtgat
    24241 cagtgcggtg aaaaagatgt cggcactggg cgtggcggtg atttatgtca gccaccggat
    24301 ggaagaaatc cgccgcattg cctcctgtgc caccgttatg cgcgatggtc aggtggcggg
    24361 cgatgtgatg ctcgaaaaca cctccacgca tcatattgtg tcgctaatgc tagggcgtga
    24421 tcacgttgat attgcgccgg ttgcccctca ggaaattgtg gatcaggccg tactggaagt
    24481 ccgtgcgtta cgccataagc ccaagctgga ggatatcagc tttacgttac gtcgcggcga
    24541 agtgcttggc attgctggcc tgctgggggc agggcgcagt gaattgctga aggcgattgt
    24601 tgggctggag acgtatgaac agggcgaaat tgttatcaac ggcgagaaaa tcacgcgccc
    24661 cgattacggc gacatgctga aacgcggcat tggctatacg ccagaaaacc gcaaagaagc
    24721 ggggatcatt ccctggttgg gcgttgacga aaatacagtg ctgaccaatc ggcaaaaaat
    24781 cagcgccaac ggagtgctgc aatggtccac catccgccgc ctgaccgaag aggtgatgca
    24841 gcggatgacg gtcaaggccg ctagtagcga aacgcccatc ggcacgcttt ctggtggcaa
    24901 tcagcaaaaa gtggtgatcg gtcgttgggt ctatgccgcc agccagattt tgttgctcga
    24961 cgagccaacg cgcggcgtcg atatcgaagc caaacagcag atttaccgta ttgtccgtga
    25021 gctggctgcc gaaggaaaaa gcgtggtgtt tatctccagt gaagtggagg agttgccgct
    25081 ggtgtgtgac cgcatcctgt tattacagca cggcacgttc tcgcaggagt ttcactcgcc
    25141 ggtcaatgtg gatgagctga tgtccgccat tctgtctgtg cactgatgaa ttttaccgag
    25201 gaacctgaag atgtctgctt cgtcattacc attgccgcag ggcaagagcg tctcgctcaa
    25261 acaatttgtc agtcgccata ttaatgagat cggtttgctg gtggtgattg ccattcttta
    25321 tctggtcttc tccctgaacg cgcctggctt tatctcattg aataaccaga tgaacgtgct
    25381 gcgcgatgcc gccaccattg ggattgccgc ctgggcgatg acgctgatta ttatctccgg
    25441 tgaaattgat gtcagcgttg ggccgatggt ggcttttgtc tcggtgtgcc tggcattttt
    25501 gctgcaattt gaagttccgt tggcgattgc ttgtctgctg gtgttgctgt taggcgcgct
    25561 gatgggaacg ctcgccgggg tgctgcgcgg cgtgtttaac gtaccaagtt tcgttgccac
    25621 actggggctg tggagcgccc tgcgcggaat ggggctgttt atgaccaacg cactgccagt
    25681 gccgattgac gaaaacgagg tgctggactg gctgggcgga caatttctcg gtgtgccagt
    25741 atccgcgctg atcatgatgg tgttgtttgc gctgtttgtg ttcattagcc gaaaaaccgc
    25801 cttcgggcgc tcggtttttg ccgttggcgg taatgccacg gcggcgcagt tgtgcggtat
    25861 taacgttcgt cgggtgcgca ttcttatctt taccctttcg ggattattag cggcggtgac
    25921 cggcattttg ctggcggcac gcctcggttc cggtaacgca ggtgccgcaa acggtctgga
    25981 gtttgacgtc atcgccgcgg tcgtggtcgg cggtacggca ctttccggcg gtcgcggttc
    26041 cctgttcggt acattgcttg gtgtgctggt gattacgcta atcggtaacg gtctggtgct
    26101 gctcggtatt aactcctttt tccagcaggt ggtacgcggc gtcatcatcg tggtggcggt
    26161 gctggcgaat atcttgctga cccagcgaag cagtaaagcg aaacgctaaa ccttaacgcc
    26221 cctggcgcgg gtgcggcagg gatgaaaatt aatcgtattg ttaatgaggg atttatgaaa
    26281 acgatgctgg cagcttattt accaggaaat tcaaccgtcg atctgcggga agttgcggtg
    26341 ccgacgccgg ggattaacca ggtactgatc aaaatgaaat cctccgggat ttgcggaagc
    26401 gatgtccact atatctatca tcaacaccgt gccacggcgg cggcacccga taaaccgtta
    26461 taccagggat ttatcaacgg tcatgaaccg tgcgggcaga ttgtggcgat ggggcaaggc
    26521 tgccgccatt ttaaagaggg cgaccgcgtg ctggtgtatc acatttctgg ctgtggtttt
    26581 tgcccgaact gccgccgcgg ctttcctatc tcttgtactg gcaaaggaaa agcggcttac
    26641 ggctggcagc gtgacggcgg tcatgccgaa tatctgctgg cggaagaaaa agatctgatc
    26701 ctcctgccgg atgcgctgag ctacgaagat ggtgcgttta tcagttgcgg cgttggcacg
    26761 gcctatgaag ggattttgcg cggcgaagtt tccggcagcg ataacgtgct ggtggtcggt
    26821 ctggggccgg tcggcatgat ggcgatgatg ctggcgaaag gtcgcggtgc aaaacggatc
    26881 atcggcgttg atatgctgcc ggaacgtctg gcgatggcaa aacagttagg ggtgatggat
    26941 cacggctatt tagcaaccac cgaaggtctg ccgcagatta tcgccgaact cacccacggt
    27001 ggcgcggatg ttgcgctcga ttgttctggt aatgccgcag gtcgcttgct ggcactgcaa
    27061 tccaccgctg actggggacg ggtggtttac attggtgaaa ccggaaaagt ggaattcgag
    27121 gtcagcgccg atctgatgca ccatcaacgg cggattatcg gctcctgggt gaccagtctg
    27181 ttccatatgg aaaaatgcgc ccacgatttg actgactgga agctgtggcc gcgtaacgcc
    27241 attacccatc gcttctcgct ggaacaggca ggtgatgcct atgcgctgat ggcgagcggc
    27301 aaatgcggga aagttgtgat taacttcccg gattaaggag ttagcggatg accatctata
    27361 ccttatccca cgggtcgcta aagctggacg tttctgacca gggcggcgta atcgaaggct
    27421 tctggcgcga tacgacgccg ctcttacgtc ctggtaaaaa aagcggtgtg gcaaccgatg
    27481 cctcatgttt tccgctggtg ccatttgcaa accgggtgag cggcaaccgt tttgtctggc
    27541 aggggcgtga gtatcaactg caaccgaatg ttgagtggga tgcgcactat ctacacggcg
    27601 atggctggct gggcgagtgg caatgtgtct cgcacagcga tgatagtttg tgtctggtgt
    27661 atgaacatcg cagcggtgtc tatcactatc gggtaagtca ggcgtttcat ttaacggcgg
    27721 atacgctgac ggtgacgctc tctgtcacca atcaaggggc agagacgctg ccatttggta
    27781 ccggctggca tccttatttt ccgttgtcac cacaaacgcg gattcaggcg caggcgagcg
    27841 gttactggct ggaacgggag cagtggctgg cgggtgagtt ttgcgagcag ctaccgcagg
    27901 aactggattt taaccagctc gcgccgttgc cgcgccagtg ggtgaacaat ggttttgccg
    27961 gatggaatgg tcaggcccgc atcgagcagc cgcaggaagg ttatgccatc atcatggaaa
    28021 cgacgccacc tgcaccgtgt tatttcatct ttgtttccga ccctgcgttt gataaaggat
    28081 atgcgtttga tttcttctgt ctggaaccga tgagccatgc gccggatgat catcatcgcc
    28141 cggaaggggg tgatctcatt gcgctagcac caggggaatc aacaacttca gagatgtcgt
    28201 tgcgggtaga gtggctgtaa tttattaaat gccggataag gcgttcacgc cgcatccggc
    28261 atctttccta acgccgatcg agagaaattg cccccggccc ggtaattgcc aatagcaaga
    28321 acgcgccagc gatactgaca ttcttccaga aattaatcat atttggccca accgcatcgc
    28381 cggtcatatc ccagtaatgg tgaccaatca ccgccgtacc cagcgtgtag aaaataaaca
    28441 gcaccgccag cggacgggtg aaaaagccaa gcacgattaa tatcgcggcg ggcacttcca
    28501 taactaccgc aataatcgct gccagcatcg gcattggcgc gcccaacgag gccatatatt
    28561 ggaccgtacc gtcaaagccc atcattttgg gaaaaccaaa aataatgaaa attaagacca
    28621 cggcgatacg ggcaattaat aacaaaacgg ggcgggcagc tccaaaatca aaataacg
//
LOCUS       DADMKF010000042        28263 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.043, whole genome shotgun sequence.
ACCESSION   DADMKF010000042 DADMKF010000000
VERSION     DADMKF010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28263
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.043"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(<1..215)
                     /locus_tag="JNC24_001641"
     CDS             complement(<1..215)
                     /locus_tag="JNC24_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAY9158405.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDT"
     gene            complement(286..2400)
                     /gene="fusA"
                     /locus_tag="JNC24_001642"
     CDS             complement(286..2400)
                     /gene="fusA"
                     /locus_tag="JNC24_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAY9158406.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2497..2967)
                     /gene="rpsG"
                     /locus_tag="JNC24_001643"
     CDS             complement(2497..2967)
                     /gene="rpsG"
                     /locus_tag="JNC24_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006714698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HAY9158407.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3064..3438)
                     /gene="rpsL"
                     /locus_tag="JNC24_001644"
     CDS             complement(3064..3438)
                     /gene="rpsL"
                     /locus_tag="JNC24_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003852912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HAY9158408.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3564..3851)
                     /gene="tusB"
                     /locus_tag="JNC24_001645"
     CDS             complement(3564..3851)
                     /gene="tusB"
                     /locus_tag="JNC24_001645"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HAY9158409.1"
                     /translation="MLHTLHRSPWLADFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQISNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(3859..4218)
                     /gene="tusC"
                     /locus_tag="JNC24_001646"
     CDS             complement(3859..4218)
                     /gene="tusC"
                     /locus_tag="JNC24_001646"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HAY9158410.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLPGQKPDAVLARDYIATFKLLGLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4218..4604)
                     /gene="tusD"
                     /locus_tag="JNC24_001647"
     CDS             complement(4218..4604)
                     /gene="tusD"
                     /locus_tag="JNC24_001647"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HAY9158411.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIVEGHELSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGVVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4604..5326)
                     /locus_tag="JNC24_001648"
     CDS             complement(4604..5326)
                     /locus_tag="JNC24_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAY9158412.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5493..6305)
                     /gene="fkpA"
                     /locus_tag="JNC24_001649"
     CDS             complement(5493..6305)
                     /gene="fkpA"
                     /locus_tag="JNC24_001649"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003827768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HAY9158413.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6526..6744
                     /locus_tag="JNC24_001650"
     CDS             6526..6744
                     /locus_tag="JNC24_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HAY9158414.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(6793..7383)
                     /gene="slyD"
                     /locus_tag="JNC24_001651"
     CDS             complement(6793..7383)
                     /gene="slyD"
                     /locus_tag="JNC24_001651"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAY9158415.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7478..7678)
                     /locus_tag="JNC24_001652"
     CDS             complement(7478..7678)
                     /locus_tag="JNC24_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative metal-binding protein"
                     /protein_id="HAY9158416.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVRKDEQVIGIFHPD"
     gene            complement(7688..9493)
                     /gene="kefB"
                     /locus_tag="JNC24_001653"
     CDS             complement(7688..9493)
                     /gene="kefB"
                     /locus_tag="JNC24_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HAY9158417.1"
                     /translation="MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGP
                     WGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKLWQLRRSIFGVGAAQVLLSAALL
                     AGLLMLTDFAWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
                     IPALALVPLLAGSADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFT
                     AATLLLVLGSALFMDALGLSMALGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFI
                     SVGMSLNLGVLYTHLLWVVISVVVLVAVKILVLYLLARLYGVRSSERMQFAGVLSQGG
                     EFAFVLFSTASSQRLFQGDQMALLLVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEEDE
                     KPWVNDDKPQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYYGD
                     ATQVDLLRSAGAEAAESIVITCNEPEDTMKLVEICQQHFPHLHILARARGRVEAHELL
                     QAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELIPMHADT
                     VQISRAREARRELEEIFQREMQQERRQLDGWDEFE"
     gene            complement(9493..10044)
                     /gene="kefG"
                     /locus_tag="JNC24_001654"
     CDS             complement(9493..10044)
                     /gene="kefG"
                     /locus_tag="JNC24_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HAY9158418.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRNVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     KELASHARAYGDWLANPLSPGGR"
     gene            10175..12088
                     /locus_tag="JNC24_001655"
     CDS             10175..12088
                     /locus_tag="JNC24_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAY9158419.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHGKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFQGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12088..13110
                     /locus_tag="JNC24_001656"
     CDS             12088..13110
                     /locus_tag="JNC24_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAY9158420.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRQVKFT
                     PYWQRLELPDGDFVDLAWSEAPAQARHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPPQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13104..13322
                     /locus_tag="JNC24_001657"
     CDS             13104..13322
                     /locus_tag="JNC24_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HAY9158421.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13376..14245
                     /locus_tag="JNC24_001658"
     CDS             13376..14245
                     /locus_tag="JNC24_001658"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HAY9158422.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14300..14704)
                     /locus_tag="JNC24_001659"
     CDS             complement(14300..14704)
                     /locus_tag="JNC24_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HAY9158423.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            15006..15638
                     /gene="crp"
                     /locus_tag="JNC24_001660"
     CDS             15006..15638
                     /gene="crp"
                     /locus_tag="JNC24_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HAY9158424.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            15689..17779
                     /locus_tag="JNC24_001661"
     CDS             15689..17779
                     /locus_tag="JNC24_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9158425.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKRLIIGASLFATCSLLTQLLLAKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTALHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDSPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(17846..19066)
                     /gene="argD/dapC"
                     /locus_tag="JNC24_001662"
     CDS             complement(17846..19066)
                     /gene="argD/dapC"
                     /locus_tag="JNC24_001662"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HAY9158426.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGEILWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYMHYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINPPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPQYKGRARDFLYAGAEAGVMVLNAGPDVMRFAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(19152..19715)
                     /gene="pabA"
                     /locus_tag="JNC24_001663"
     CDS             complement(19152..19715)
                     /gene="pabA"
                     /locus_tag="JNC24_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HAY9158427.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHSGEGVFRGLANPLTVTRYHSLVVEPDSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHR"
     gene            complement(19747..20349)
                     /locus_tag="JNC24_001664"
     CDS             complement(19747..20349)
                     /locus_tag="JNC24_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HAY9158428.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSKAGESE"
     gene            complement(20339..20506)
                     /locus_tag="JNC24_001665"
     CDS             complement(20339..20506)
                     /locus_tag="JNC24_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HAY9158429.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRRHYER"
     gene            complement(20611..21183)
                     /gene="ppiA"
                     /locus_tag="JNC24_001666"
                     /gene_synonym="rotA"
     CDS             complement(20611..21183)
                     /gene="ppiA"
                     /locus_tag="JNC24_001666"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HAY9158430.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGDPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21454..22635
                     /gene="tsgA"
                     /locus_tag="JNC24_001667"
     CDS             21454..22635
                     /gene="tsgA"
                     /locus_tag="JNC24_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HAY9158431.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSTA
                     MFILGVVSGITMSIGTFLITQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKHAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPAHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            22897..25440
                     /locus_tag="JNC24_001668"
     CDS             22897..25440
                     /locus_tag="JNC24_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase large subunit"
                     /protein_id="HAY9158432.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25437..25763
                     /gene="nirD"
                     /locus_tag="JNC24_001669"
     CDS             25437..25763
                     /gene="nirD"
                     /locus_tag="JNC24_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HAY9158433.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            25889..26695
                     /gene="nirC"
                     /locus_tag="JNC24_001670"
     CDS             25889..26695
                     /gene="nirC"
                     /locus_tag="JNC24_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HAY9158434.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHAMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            26714..28087
                     /gene="cobA"
                     /locus_tag="JNC24_001671"
     CDS             26714..28087
                     /gene="cobA"
                     /locus_tag="JNC24_001671"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HAY9158435.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDTLNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMSERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
BASE COUNT         6840 a         7593 c         7129 g         6701 t
ORIGIN      
        1 gtgtcatatt caacgtgaga agtgttgatg gtgataccac gagctttttc ttccggcgcg
       61 ttatcgatct ggtcgaatgc acgagcagca ccgccgtagg ttttagccag tacggtggtg
      121 attgcagcgg tcagagtagt tttaccgtgg tcaacgtggc cgatagtacc aacgttaacg
      181 tgcggttttg tacgttcaaa tttttcttta gacacggcta tattccttac tatagtgctc
      241 tccccttcag gagagagcac gggactttgg tattaaccct taggcttatt taccacgggc
      301 ttcaattacg gcctgagcaa cgttactcgg cgcttcatca tacttcagga attccatagt
      361 gtatgatgca cgacctttgg tcagagaacg cagctgagtt gcgtatccga acatttcaga
      421 cagcggtact tcagcgtgga tcttaacgcc agtaacttca gattcctgac ctttgagcat
      481 accacgacga cggctcaagt caccgataac gtcaccggtg ttctcttccg gagtttctac
      541 ttcaaccttc atgatcggct caagcagaac tggtttcgct ttcttaaagc cttctttaaa
      601 ggcgatagaa gcagccagtt taaacgccag ttcagaggag tcaacgtcat ggtaagaacc
      661 gaagtgcaga cgaataccca tgtctactac cgggtagcct gccagcggac ctgctttcag
      721 ctgttcctgg atacctttat caacggccgg gatgtattcg ccagggatta caccaccttt
      781 aatgtcgttg atgaactcgt agcctttcgg gtttgaaccc ggctccagcg ggtacatgtc
      841 gataacaaca tgaccatact gaccacgacc accagactgt ttcgcgtgtt taccttcaac
      901 atcggtaact ttctggcgga tagtttcacg gtaagcaacc tgcggtttac ctacgttcgc
      961 ttcaacgttg aattcacgct tcatacggtc aacgatgatg tcgaggtgca gttcgcccat
     1021 acccgcgatg atggtctggt tagattcttc gtcagtccat acacggaaag acgggtcttc
     1081 tttagccaga cggcccagag ccagacccat tttttcctgg tcagctttgg ttttcggttc
     1141 aactgcgatg gagattaccg gctcagggaa ttccatacgt tccagaatga tcggcgcatc
     1201 cgggtcacac agggtgtcac cagtggttac gtctttcaga ccgatagcag cagcgatgtc
     1261 gcccgcgcga acttctttga tctcttcacg tttgttagcg tgcatctgaa cgatacgacc
     1321 gaaacgctca cgtgcagctt tcacggagtt cagtacggta tcaccagagt taaccacacc
     1381 ggagtaaaca cggaagaagg tcaggttacc aacaaacggg tcggtagcga ttttgaacgc
     1441 cagtgcagag aacggctcgt catcacttgc gtgacgctca gccggagtgt ctttaccgtc
     1501 gtccaggata ccgttgatcg caggtacgtc aaccggggat ggcaggtaat caattaccgc
     1561 atccagcatc gcctgaacac ctttgttctt gaacgcagaa ccacaggtta ccaggatgat
     1621 ttcgttgttc agaacgcgct gacgcagagc acctttgatt tctgcttcag tcagttcttc
     1681 accacccagg tatttttcca tcagctcttc agaagcttca gctgcggatt cgatcaggtt
     1741 ctggtgccat tcgttagcca gttcaaccat gtctgccggg atatcttcgt attcgaaggt
     1801 tacgccctgg tcagcgtcgt tccagttgat agctttcatt ttcaccaggt caacaacacc
     1861 ggtgaaatgt tcttcagcac caatcgccag ctgcagcgga accgggttcg cgcccagacg
     1921 ggttttgatc tggttaacaa ctttcaggaa gttcgcaccc atgcggtcca ttttgttaac
     1981 gaacgcaatg cgcggaactt tatatttgtt tgcctgacgc catacggttt cagactgcgg
     2041 ctgaacacca ccaactgcgc agtaaaccat taccgcacca tcgagaacac gcatggaacg
     2101 ttctacttcg attgtgaagt caacgtgccc cggggtgtcg atgatgttga tgcgatgcgg
     2161 ctcatactgc ttagccatac cagaccagaa tgcagtagtc gcagcggaag tgatggtaat
     2221 accacgttcc tgctcctgct ccatccagtc catggttgca gcgccgtcat gaacttcacc
     2281 gattttatgg tttacaccgg tgtagaacag aatacgttcg gtagtagtgg ttttaccggc
     2341 gtcgatgtgc gcactgatac cgatgttacg gtagcgtgcg atgggtgttg tacgagccat
     2401 ttgtttcctc gtttatcttt taggcgttca atttaagtag cccaaagcgg gctgcttact
     2461 ggaagcgccc gcctggtgac taaaactccg aagggattac caacggtagt gtgcgaacgc
     2521 cttgttggct tcggccatac ggtgaacgtc ttcacgtttc ttaactgcag tacctttgtt
     2581 ttctgcagca tcagaaagtt cgttcgccag gcgcagagcc atggatttat caccgcgttt
     2641 acgagcagct tcaacgatcc aacgcattgc cagagcatta cgacgaaccg gacggacttc
     2701 aactggtacc tgataagtag aaccaccaac gcggcgagac ttaacttcta cagtcgggcg
     2761 cacgttttcg agagctactt cgaatgcttc cagttcagat ttaccagagc gctgagccag
     2821 ggtctccagc gcgctgtata cgatagattc agcagtagat tttttaccat ctaccatcag
     2881 gatatttaca aatttagcca gcagttctga tccaaacttc ggatccggca gaattttacg
     2941 ctgaccaatg acgcgacgac gtggcatgga aatactccgt tgttaattca ggattgtcca
     3001 aaactctacg agtttagttt gacatttaag ttaaaacgtt tggccttact taacggagaa
     3061 ccattaagcc ttaggacgct tcacgccata cttggaacga gcctgcttac ggtctttaac
     3121 gccggagcag tcaagcgcac cacgtacggt gtggtaacga acacccggga ggtctttaac
     3181 acgaccgcca cggatcagga tcacggagtg ctcctgcagg ttgtgacctt caccaccgat
     3241 gtaggaagtc acttcgaaac cgttagtcag acgaacacgg catactttac gcagcgcgga
     3301 gttcggtttt ttaggagtgg tagtatatac acgagtacat acgccacgtt tttgcgggca
     3361 tgcttccagc gcaggcacgt tgcttttcgc aactttgcga gcacgtggtt tgcgtaccag
     3421 ctggttaact gttgccatta aatagctcct ggttttagct tttgcttcgt aaacacgtaa
     3481 taaaacgtcc tcacacaata tgaggacgcc gaattttagg gcgatgccga aaagatgtca
     3541 agaaatatac aacgatcccg ccatcaccag gccatctggc tggagtgctt aaccgtaagt
     3601 ctgacgaaat cagtatagtc aatgggaatg atgtcgttcg aaatttgacc agtcaaaccg
     3661 cgggcaataa ggtcttcgtt cagggcatag accttaatgg gggcattacg cagactttca
     3721 aggtagcggt taccgtcaac tgcggcagtt acgccatctt gcaataatag cagttcgtcg
     3781 ccttcactga gcagacgcag cagcgcagca aaatccgcca gccagggtga acgatgtaat
     3841 gtgtgcagca taaaagcctc aaaacctcaa aataacatcg tagttggcga gttcgcggcg
     3901 taaggcatct gcttcgagcg gcgtggcttc gacaacaaag ggggtctgcg gatctaaccc
     3961 gcgttcgcgc agtgaagccg cacaaaccca gcactgttca atgtcgtaca gacccaacaa
     4021 tttaaaagtg gcaatgtaat cacgcgccag cactgcatcg ggcttttgtc ctggcagcag
     4081 ctgaaaaacg ccatcagcta taaagaagac agccagatca tcagttaatg cggaagttgc
     4141 cagtaaagca tctaaacctt cccgaccagc agctgtacca tgaggcgcag tagaaaaaac
     4201 aaacgcaatt cgtttcatca gaactgtacc accctgtcac aggtcagcga ggcttccgcc
     4261 agcgcgccaa gtccgcttaa ggtaaatccc ggctgaaggt ttgacgaggc cagccccagc
     4321 cttccggcct ccgtttcatc aacaacgcca cggcgtaatg ctgccgctac gcagatattc
     4381 agcgccacac catgttgcgc attcaactgt tgccagccgc gcacaaggtc aaattcgtcg
     4441 cttgccggtg aggtcaattg gttagcgtta tagacccctt cccgataaaa aaagacgctg
     4501 cttaactcat ggccttctac tatcagcgcc tgcgcaaact gaaaagcact actcgcctgt
     4561 tgcgtaccgt atgctggccc ggtcaccacg atggcaaaac gcattactta tcttgcccct
     4621 ggaaatcacc gcttttgaac tggcggatgt agagatagac agtgtgtttg gagatgttca
     4681 ggcggtcagc aacctggttg atcgcgtctt taatatcgaa gatccctttc tcgtagagat
     4741 tcagcacgat ctgacgattt ttggcgttat tagaaacatt gcgatcggca tttacttctt
     4801 cgatggtgaa ctccagcgtt tgggtaacca gatcttcaac agaagaggca aagttgacgc
     4861 ttgaaccgac atccggggtt tctggcggca caaaagtgct cataatctgc gagaagggaa
     4921 catcaagatt catattgatg cacagcagac caattacacg ctgttcgcgg ttacgaatcg
     4981 cgatagtcag ggacttcatt aatacgccgc ttttggcgcg agtaaagtag catttagaaa
     5041 cgctgctatc cgctcccgtc atatcgtgca gcatacgtag cgccaggtca gtaattggcg
     5101 aaccaatctt ccggcctgta tgttcaccgt tagcaatgcg aatggctgaa cattttagat
     5161 cctgcaaaga gtgcaaaacg atttcacagt gggagccaat aagcatcgct aacccgtcca
     5221 ccaccgcttc gtaggatttc agaatatcaa aatcggtctg gtcgaaagga cgttgatcca
     5281 gtaaatccaa ctcactggtt tcgttggtta aaagcgacct ggacatgaaa aaaaacactc
     5341 cttttcagga gcctgtcgtt aacttttcag ggcaggctca ttaatgatgc gggtaactaa
     5401 attaatacag cggaggttcc gctttccagc actaattata tccggcctgt aataaaaaaa
     5461 ccgccgcctg gtcaggcggc ggttcttaat gcttattttt tagcagaatc tgcggctttc
     5521 gcatcagctt ccggctttgc atcagccttc ggcgctggtt tcacatccag cagctctacg
     5581 tcaaacacca gggtagaatt cggtgggatc cccggaacac ccgctttgcc gtaagccagt
     5641 tctggtggaa taaccagttt gatcttaccg cctttcttga tgttcttcag accttctgtc
     5701 caccccggga taacaccgtc cagacggaaa gagagcggtt caccacgggt gtaagagttg
     5761 tcgaactctt taccgtcgat cagcgtacct ttgtagttca ctacaacagt atcgctgtct
     5821 ttcggtgctt cgcctttacc ggcttctact acctgataaa ccagaccagt tgaagaggtt
     5881 ttcacacctt tctctttggc aaatttctcg cggtactctt taccttttgc ttcgttatca
     5941 gccgcgtctt tttccatctt cgcctgagca gaagacttca cgcgagcttc gaatgcttgc
     6001 agagtctgtt cgatctcttg gtcggagagt ttgctcttat cagcaaatgc atcctgaaca
     6061 ccagcgatca gctgatcttt atccagtttg atgcccagtt tttcttgttc tttaagagag
     6121 ttttccatgt aacgacccag cgaagcaccc agtgcataag ctgatttctg atcgtcattt
     6181 ttgaacgctg ctttgctgtc agcagttgta gcaggttttg cagcttcagc agcaaaagtg
     6241 attggtgcat gcagggcaac ggccattgtg gtcgccagca gcgttacttt aaacagtgat
     6301 ttcatccata tctccaggat cggggcatct caccccaggg ttaactatta tcagaagggt
     6361 actataaagc gttgtcgaac aaatctacat acagacacgc cctattatca tctattttca
     6421 gactcttttt gtttaaatta gtttcgatga ccgcgaaatg agtgctgtct cgggcagcaa
     6481 agttaagtag aatccgcggc gaccattcga caaaagaggt gaatcatgca ggatttatca
     6541 ttggaagcac gcctggcaga gctggagagc cgactggctt ttcaggaaat caccattgaa
     6601 gaactgaacg tcacggtgac cgctcatgaa atggagatgg cgaaactgcg cgatcatctg
     6661 cgtctgctga ccgagaagtt aaaagccagc cagccgtcga acatcgcgtc gcaggctgaa
     6721 gaaacgccac cgccacatta ttgaggcgta aaaaaagcgg ggattccccg cttttttgtc
     6781 accttttcgg tattagtggc aaccacaacc gccgttgcct ttaccgccac agcagccttc
     6841 gccaccgtgt tcatgaccgt gatcgtggcc atgaccgccg cagcaaccgt cgtggtcgtg
     6901 atcgtggtga tgatcgtgcg cgccgtgaac gtgaccatga gccagttctt cttcggtcgc
     6961 ttcacgaatc gccacaacct caacgttgaa tttcaggttc tgaccggcca gcatgtggtt
     7021 accatcaacc actacgtgat cgtcttcaac cgcagtgatt tcaaccggta ccggaccctg
     7081 gtcggtttca gccaggaaac gcatacctac ctgcagttca tcaacgccca taaatacgtc
     7141 tttaggaaca cgttgcacca ggttttcgtc gtactgaccg taagcgtcgt tcgcgccaac
     7201 agcgacatca aatttgtcgc caacttcatg accttccagc gccgtttcca ggccagagat
     7261 cagggaaccg tgaccatgca ggtagtccag cggcgcactc accggagact catcaaccaa
     7321 cacaccgtct tctgtacgta cctgataggc caggctgacc accaggtctt ttgctacttt
     7381 catgatatct cctgagcatg ggaagaatag tggcgcagat tgtagcggaa ttctgcagcc
     7441 gtgtactcac tagcttaaaa aaactcggcg catatcgcta gtccggatga aaaatcccga
     7501 tcacttgctc atctttgcga acgtgatcgc gggcttcttt gtctgcttct cgcatctgat
     7561 gtccgcactt aacgcattca acaatatcaa tattattttc gcgccacatc gccattgaat
     7621 cctgtgcctg acaggccggg cattttgcgc ccgcaataaa acgttttcgg attgccatct
     7681 ttaccctcta ctcaaattca tcccagccgt ccagctggcg tcgttcttgt tgcatttcac
     7741 gctggaaaat ctcttccagt tcgcgtcggg cttccctggc gcgagaaatt tgtacagtat
     7801 cggcatgcat tgggatgagc tctcgcagca tccgcatatc caggcggcga aaatgcagtt
     7861 gcgcccgctg cgcctgatgc ggatgcatcc caagcgtgac caaagtcttg cgccccagct
     7921 ctaacgcact ggagaatgtt tcacgggaaa actgcgtcac ccctgcctgt aataactcat
     7981 gcgcttccac acgtccgcgc gctcgcgcaa gaatatgcaa atgcggaaag tgctgctggc
     8041 atatttccac cagcttcatg gtgtcttccg gctcgttaca ggtaatgacg atagactcag
     8101 cggcctctgc acccgcagaa cgtaatagat cgacctgcgt ggcgtcgccg taataaactt
     8161 tgtagccata tttacgcatc aggttaacgg cgctgatatc ccgctccagc acggtaatgc
     8221 gcattttatt tgccatcagc aaacgaccaa tcacctgacc aaaacgcccg aagcccacga
     8281 caatgacctg gggtttatca tcgttgaccc acggtttttc gtcttcttct tccggtccgt
     8341 taaactggcg ggatagccat ttatccacca gcttcatcag caacggcgtg gtcatcatgg
     8401 aaagcgtcac cgtcaccagc aacagcgcca tctggtcgcc ctggaataaa cgttgtgaag
     8461 aagcggtaga aaagaggaca aaggcaaact caccgccctg actcaacacg ccagcaaact
     8521 gcatccgctc tgagctacgc acgccataca atcgcgccag cagatacagc acgagaattt
     8581 tcaccgccac cagcacaacc acgcttatca ctacccacaa cagatgggta taaagcaccc
     8641 cgaggttgag cgacatgccg acagagataa agaacaaacc gagcagcaag cctttgaagg
     8701 gatcgatagc cgtttccagt tcatggcgat attcactttc cgccagcagc acgcccgcaa
     8761 taaatgtacc gagcgccatc gacagcccca gcgcatccat aaacaatgcg gaacccaaca
     8821 ccagcagcag cgtcgcggcg gtgaacactt cccgcacgcc agaagctgca ataaagcgga
     8881 ataccggacg cagtaaatag cgcccaccaa tcagcatgcc gacaaacgcc agcaccttca
     8941 tgccgatctt catccagtcg aaatgttcgt ctgccgaccc cgccaacaac ggcactaacg
     9001 ccagtgctgg gatcactgcc agatcctgaa acagcagaac cgaaaacccg agctggccgg
     9061 attcgctgcg attcatccct ttctcacgca tcaattgcaa cgccattgca gttgaagaca
     9121 tcgcaaggcc aatgccaccg accaccgccg cctgccaggc gaaatccgtc agcatcaata
     9181 atcccgccag caacgccgcg cttaacagca cctgtgccgc gcctacgcca aaaatcgaac
     9241 gccgcagttg ccaaagtttg gagggattca actcaaggcc gatgataaac atcaggaata
     9301 ccacgccgag ttccgaaaag tggaggatct catcgacgtc gctaataaac cccagccccc
     9361 acgggccaat tgcaatccct gccagcaaat atcccaacac agcgccaatc cccagccgcg
     9421 atgccaacgg caccgcagcc accgccgcga agagaaacag cactcctgcg agtaaaaaat
     9481 cggaaccttc catcagcggc ctcctggaga cagcggattt gccagccagt caccgtaggc
     9541 tctggcgtgg ctcgccagct cctttgcgct ttgccgtctc gcccagtaaa taatgatggg
     9601 acttaaccaa tgcatccggc acatgcccgc cgccagttca aaggggcgca gcacatcgct
     9661 catcgggtag cgattcagcg cgtcataacg gtaagcactt tccggctcgc cggtggtaat
     9721 cacgttacgc cagtactttc ccgccagttg gtttcctccc ggcccgctgg caaaaccacg
     9781 acttaatacc cggtccagcc actctttcag tagcgccggg cagctatagg tataaagagg
     9841 atgctgaaag acaatcacct cgtgctcgcg cagtaatgcc tgctcacggg ggatatcaat
     9901 aaaaaaatcg ggatagtgcg cgtaaaggtc gtgcacggta acattgctga gctgcgtggc
     9961 cggtttaagc agtacccggt ttgccaccga gtcctgagat tccggatggg catacagcag
    10021 caaaactttc gctggctgag acatcatccc cctcccggta tggtttttgt gtatagtcgc
    10081 tgttttgggc taccattgcg cccggtgcgg cagctcgccc atacattaca ttatcataat
    10141 gataagttaa catagtctga acatacggcg ccttatgatt gttttctcct cgttacaaat
    10201 tcgtcgcggc gtgcgcgtcc tgctggataa tgccaccgcc accatcaacc ccgggcagaa
    10261 agtcggcctg gtgggtaaaa acggctgtgg taaatctacc ctgctggcat tgctgaaaaa
    10321 tgaaatcagc gccgacggcg gcagctacac ctttccggga agctggcaac tggcgtgggt
    10381 gaatcaggaa acgccagcgt taccgcaagc ggcgctggaa tatgtcattg acggcgaccg
    10441 tgaatatcgt caactggaag cgcagctgca cgacgccaac gaacgtaacg acgggcacgc
    10501 cattgcgacc attcatggca agctggatgc tattgacgca tggagtattc gctcccgtgc
    10561 cgccagcctg ctgcacggcc tcggtttcag caatgaacaa ctggagcgcc cggtaagtga
    10621 tttctccggt ggctggcgta tgcgtcttaa ccttgcccag gcgctgattt gccgttcaga
    10681 cttgctgctg ctcgacgaac cgactaacca cctcgatctc gatgccgtta tctggctgga
    10741 aaaatggttg aagagctatc agggcacgct gatcctgatc tctcacgacc gcgacttcct
    10801 cgatccgatc gttgataaaa ttattcatat cgaacaacaa agcatgttcg agtacaccgg
    10861 caactacagt tcgtttgaag tacagcgcgc cacccgtctg gcgcagcaac aagcgatgta
    10921 tgaaagccag caggaacgcg tggcacatct gcaaagttat atcgaccgtt tccgtgccaa
    10981 agccaccaaa gcgaagcagg cccagagccg cattaagatg cttgagcgta tggagctgat
    11041 tgcccccgcg cacgtcgaca acccgttccg ctttagcttc cgcgcgccgg aaagcctgcc
    11101 aaatccgtta ctgaagatgg aaaaagtcag cgcgggctat ggcgatcgca ttattctcga
    11161 ctcgattaaa ctgaacctgg tgcccggctc gcgcattggt ctgttaggcc gcaacggcgc
    11221 gggtaaatcg acattaatca aactgttagc cggtgaactt gcgccagtca gcggtgaaat
    11281 tggtctggcg aaagggatca agctcggcta cttcgcccag catcaacttg aatacctgcg
    11341 cgccgacgaa tcacctattc aacatctggc acgtttagcg ccgcaggagc tggaacaaaa
    11401 actgcgtgac tacctcggcg gctttggttt ccagggcgat aaagtaaccg aagaaacgcg
    11461 ccgcttctcc ggtggggaaa aagcccgcct ggtgctggca ttaattgtct ggcagcgtcc
    11521 gaatctgctg ctgctcgacg aaccgactaa ccaccttgac ctcgacatgc gtcaggcact
    11581 caccgaagca ttaatcgagt ttgaaggcgc gctggttgtc gtttcgcacg accgtcattt
    11641 gctgcgttcc accactgacg atctctacct ggttcacgat cgtaaagtcg aaccgttcga
    11701 cggcgatctg gaagattatc aacagtggtt gagcgacgta caaaagcagg aaaaccagac
    11761 cgacgaagcg ccaaaagaga acgcgaacag cgcccaggca cgtaaagatc agaagcgtcg
    11821 ggaagcggag ctgcgtgcgc aaacccagcc gctgcgtaaa gagattgccc gtctggaaaa
    11881 agagatggag aagctgaacg cgcaactggc gcaggcggaa gagaaactcg gagacagcga
    11941 actgtatgac cagagccgta aagcggagtt gaccgcctgc ctgcaacagc aagccagcgc
    12001 caaatccggc ctggaagagt gcgaaatggc gtggctggaa gcccaggagc agcttgagca
    12061 gatgctgctg gaaggccaaa gcaactgatg gcgcagataa cgacgaccga tgccaatgaa
    12121 ttcagcagca gtgctgaatt cacccctatg cgcggcttta gcaattgtca tctacaaact
    12181 atgctgccgc gtttgtttcg tcgtcaggtg aaattcaccc cgtactggca gcggctggag
    12241 ttgcccgacg gcgattttgt cgatctcgcg tggagtgaag cccctgcaca ggctcggcat
    12301 aaaccgcgtt tagtggtatt tcacgggctg gaaggcagtc tcaatagccc ttacgcccac
    12361 ggtctggttg aggcggcgca aaagcgcggc tggctgggcg tggtgatgca ttttcgcgga
    12421 tgcagcggtg aaccaaaccg tatgcaccgc atttaccatt cgggcgaaac cgaagacgcc
    12481 agttggtttt tacgctggct gcaacgcgaa tttggtcatg cgccaacggc tgccgtcggc
    12541 tattcgctcg gcggtaatat gctggcctgt ttgctggcaa aggaaggcaa tgatctcccg
    12601 gttgatgcgg cggtgattgt ctctgcgccg tttatgctgg aagcctgtag ctatcatatg
    12661 gaaaagggct tttcccgcgt ttatcagcgt tacttgctga acctgttaaa agccaatgcc
    12721 gcgcgcaagc tggcagccta ccccggaacg ctgccgatta atctcgcgca gttaaaatcg
    12781 gtacgtcgca tccgtgaatt tgacgatcta atcaccgcca gaattcacgg ctacgccgac
    12841 gctatcgact attatcgtca gtgtagcgcc atgccgatgc tgaaccggat cgccaaaccg
    12901 acgctgatta ttcacgccaa agacgatccg tttatggatc atcaggtgat cccgaaaccg
    12961 gaaagtctcc ccccgcaggt ggagtatcaa ctgactgaac atggcggtca tgttggcttt
    13021 attggcggta cattacttca tccgcaaatg tggctggagt cacgcattcc tgactggtta
    13081 acaacgtatc tggaggcgaa atcatgttga ttccgtggca agacctctcc cccgaaacgc
    13141 tggaaaattt aattgaaagc tttgtgttac gtgaaggcac cgattatggt gaacatgagc
    13201 gtacacttga acagaaagtc gccgacgtca aacgccagct acagtgcgga gaagcggtgc
    13261 tggtatggtc ggagctgcac gaaacggtca atatcatgcc gcgcagtcag tttcgcgaat
    13321 agcctgcaac cgtcggcgga cgagataata atgatagcta agtcatggag ttactatgtc
    13381 tgccaaacat ccggtcattg cggtaacagg atccagcggc gcggggacca ccaccaccag
    13441 cctcgcgttt cgtaaaatat tcgcgcagtt aaatctgcat gcagctgagg tggaaggcga
    13501 cagttttcac cgttacaccc gcccggaaat ggacatggcg atccgcaaag cgcgcgacgc
    13561 cgggcggcat atcagctact tcggccccga ggctaacgac ttcggcctgc tggaacaaac
    13621 cttcattgaa tacggtcaga gcggcaaagg gaaatctcgc aaatatctgc atacctacga
    13681 cgaagccgta ccgtggaatc aggtaccggg gacattcacc ccctggcaac ctttaccgga
    13741 acccactgat gtactgtttt atgaaggttt acacggcggc gtagtcacgc cacagcataa
    13801 cgttgcgcag catgtggact tactggtcgg cgtggtgcct atcgttaacc ttgagtggat
    13861 tcaaaaactg atccgcgaca ccagcgagcg cgggcactca cgagaagcag tgatggactc
    13921 agtagtgcgt tcaatggaag actatatcaa ctacatcaca ccgcagtttt cccgcaccca
    13981 tcttaacttc cagcgcgttc ccaccgtcga cacttcaaac ccgttcgcgg caaaaggtat
    14041 cccgtcgctc gatgaaagct ttgtggtgat ccattttcgt aatctggaag ggatcgattt
    14101 cccctggctg ctggcgatgt tgcaaggctc attcatttcc cacatcaata cgttagtggt
    14161 accgggcggc aaaatgggtc tggcaatgga attaattatg ctgccgctgg tgcaacgatt
    14221 gatggaagga aagaaaatcg agtaactctg ctattacgcc ggataaaata ctatccggct
    14281 tcacaacggg atagttaaat cacgcggcaa ccacttcata cgagtgagta atattcaccg
    14341 ctttttccag catcaacgcc actgaacaat atttctcagc agagagatca accgcacgcg
    14401 caaccgctgc gtctttcagg tcgcgaccgg tgacgataaa atgcagatta atgtgcgtaa
    14461 acaggcgtgg tgcctcttcg cggcgttcag aggtcaattt tacttcacaa tcgaccacat
    14521 cctgacgccc tttttgcagg atagaaacca catcgatggc actgcaacca cccgccgcca
    14581 tcaacaccat ttccatcgga cttggtgctt tatcgcctga gttgccgtcc attaaaatct
    14641 ggtgaccaga ggcggattcg cccagaaaag ttaacccttc gacccacttc actcgcgctt
    14701 gcatttttgc tactccactg cgtcaatttt cctgacagag tacgcgtact aaccaaatcg
    14761 cgcaacggaa ggcaacctgg gtcatgctga agcgagacac caggagacac aaagcgaaag
    14821 ctatgctaaa acagtcagga tgctacagta atacattgat gtactgcatg tatgcaaagg
    14881 acgtcacatt accgtgcagt acagttgata gccccttccc aggtagcggg aagcatattt
    14941 cggcaatcca gagacagcgg cgttatctgg ctctggagaa agcttataac agaggataac
    15001 cgcgcatggt gcttggcaaa ccgcaaacag acccgactct cgaatggttc ttgtctcatt
    15061 gccacattca taagtaccca tccaagagca cgcttattca ccagggtgaa aaagcggaaa
    15121 cgctgtacta catcgttaaa ggctctgtgg cagtgctgat caaagacgaa gagggtaaag
    15181 aaatgatcct ctcctatctg aatcagggtg attttattgg cgaactgggc ctgtttgaag
    15241 agggccagga acgtagcgca tgggtacgtg cgaaaaccgc ctgtgaagtg gctgaaattt
    15301 cgtacaaaaa atttcgccaa ttgattcagg taaacccgga cattctgatg cgtctgtctg
    15361 cacagatggc gcgtcgtctg caagtcactt cagagaaagt gggcaacctg gcgttcctcg
    15421 acgtgacggg ccgcattgca cagactctgc tgaatctggc aaaacaacca gacgctatga
    15481 ctcacccgga cggtatgcaa atcaaaatta cccgtcagga aatcggtcag attgtcggct
    15541 gttctcgtga aaccgtggga cgcattctga agatgctgga agatcagaac ctgatctccg
    15601 cacacggtaa aaccatcgtc gtttacggca ctcgttaatc ccgtcggagt ggcgcgttac
    15661 ctggtagcgc gccattttgt ttcccccgat gtggcgcaga ctgatttatc accccgatat
    15721 caactatgca cttcgacaaa cgctggtgct atgtttgccc gtggccgttg ggttaatgct
    15781 tggcgaatta cgattcggtc tgctcttctc cctcgttcct gcctgttgca atattgcggg
    15841 ccttgatacg cctcataaac gttttttcaa acgcttaatc attggtgcgt cgctgtttgc
    15901 cacctgtagc ttgctgacac agctactact ggcaaaagat gttcccctgc cctttttgct
    15961 gaccggatta acgctggtac ttggcgtcac tgctgagctg gggccattgc acgcaaaatt
    16021 gcttcctgca tcgctgctcg ccgccatttt taccctcagt ctggcgggat acatgccggt
    16081 ctgggaaccg ttgctcatct atgcgttggg cactctctgg tacggattgt ttaactggtt
    16141 ttggttctgg atctggcgcg aacaaccgct gcgcgagtca ctaagtctgc tgtaccgcga
    16201 actggcagat tattgtgaag ccaaatacag cctgcttacc cagcacaccg accctgaaaa
    16261 agcgctgccg ccgctgctgg tgcgccagca aaaagcggtc gatctaatta cccagtgcta
    16321 tcagcaaatg catatgcttt ccgcgcaaaa taatactgat tacaagcgga tgctgcgtat
    16381 tttccaggag gcgctggatt tacaggaaca tatttcggtc agtttgcatc agccggaaga
    16441 ggtgcaaaag ctggtcgagc gtagccatgc ggaagaagtt atccgctgga atgcgcaaac
    16501 cgtcgccgct cgcctgcgcg tgctggctga tgacattctt taccatcgcc tgccaacgcg
    16561 ttttacgatg gaaaagcaaa ttggcgcact ggaaaaaatc gcccgccagc atccggataa
    16621 tccggttggg caattctgct actggcattt cagccgcatc gcccgcgtgc tgcgcaccca
    16681 aaaaccgctc tatgcccgtg acttactggc cgataaacag cggcgaatgc cgttacttcc
    16741 ggcgctgaaa agttatctgt cactaaagtc tccggcgcta cgcaatgccg gacgactcag
    16801 tgtgatgtta agcgttgcca gcctgatggg caccgcgctg catctgccga agtcgtactg
    16861 gatcctgatg acggtattgc tggtgacaca aaatggctat ggcgcaaccc gtctgaggat
    16921 tgtgaatcgc tccgtgggaa ccgtggtcgg gttaatcatt gcgggcgtgg cgctgcactt
    16981 taaaattccc gaaggttaca ccctgacgtt gatgctgatt accaccctcg ccagctacct
    17041 gatattgcgc aaaaactacg gctgggcgac ggtcggtttt actattaccg cagtgtatac
    17101 cctgcaacta ttgtggttga acggcgagca atacatcctt ccgcgtctta tcgataccat
    17161 tattggttgt ttaattgctt tcggcggtac tgtctggctg tggccgcagt ggcagagcgg
    17221 gttattgcgt aaaaacgccc atgacgcttt agaagcctat caggaagcga ttcgcttgat
    17281 tcttagcgag gatccgcaac ctacgccact ggcctggcag cgaatgcggg taaatcaggc
    17341 acataacact ctgtataact cattgaatca ggcgatgcag gaaccggcgt ttaacagcca
    17401 ttatctggca gatatgaaac tgtgggtaac gcacagccag tttattgttg agcatattaa
    17461 tgccatgacc acgctggcgc gggaacaccg ggcattgcca cctgaactgg cacaagagta
    17521 tttacagtct tgtgaaatcg ccattcagcg ttgtcagcag cgactggaat atgacgaacc
    17581 ggggagttct ggcgatgcca atatcatgga ttcgccggag atgcagccgc acgaaggcgc
    17641 ggcaggtacg ctggagcagc atttacagcg ggttattggt catctgaaca ccatgcacac
    17701 catttcgtcg atggcatggc gtcagcgacc gcatcacggg atttggctga gtcgcaagtt
    17761 gcgggattcg aaggcgtaat gcaggctaaa ccatcggata aggcattcac gccgcatccg
    17821 acatcttttg cctgatgctt cgtatttacg ccccaaccac cttcgccacc gcgtgggcga
    17881 aacgttgcat cccttcatcg atatccgcat cttccaccac cagcgacggt gcaaaacgca
    17941 tcacatccgg tccggcattc agcaccatta cgccagcctc tgcgcccgca tacaggaaat
    18001 cacgcgcccg acctttgtac tgtggtttca gctctgcgcc aatcaacagc cccataccgc
    18061 gaatatcgct aaacacatcg tactgctgat cgatcttctg cagatggtca acaaaacgct
    18121 ggcgtttcgc ctgaatgcct tccagcactt cagggggatt gatgatatca aacgccgccc
    18181 ccgctactgc acaggccaga ggattaccgc cgtaggtgga accgtgagaa ccaggatgaa
    18241 acgcagaagc aatttccgcc gtggtcagca tggcgctaat cgggaagccg ccgcctaacg
    18301 ctttcgcaga ggtcagaata tccggcgtca cgccgtagtg catgtaagca aacaaatcgc
    18361 cggtccgccc catcccgcac tgcacttcat caaacaccaa taatgcctga tgttgatcgc
    18421 acagctcgcg caagccctgc aaaaactctg gcgtcgcagc cgtcacgccg ccctcgccct
    18481 ggatcggctc aaccaccacc gcacaggtgt gatcatccat cactgctttc actgcgtgga
    18541 gatcgttaaa gggaacgtgg atgatgtctg ccggtttcgg cccaaagccg tcggaatatt
    18601 ttggctgccc acccaccgaa acggtaaaca gcgagcgacc atgaaaagcg ttatggaagg
    18661 caataatttt ggttttgaac gggctatgac gcacacaggc gtaatgacgt gccagtttaa
    18721 aggcggtttc gttagcttcc gtgccggagt tcataaacac cacgcgttcg gcaaacgttg
    18781 cctcaatcag tttacgccca agacgcagcg ccggttcatt ggtgaaaacg ttactgatat
    18841 gccacagaat ttcgccctgg gtttttaacg cgttcaccag cgcaggatgg caatggccca
    18901 acgccgtaac tgcaatgcca cccgcgaaat cgacatactc cttgccttgc tgatcccaga
    18961 ttcggctgcc ctgacctttt accggaataa actctgccgg agcataaatc ggcaggatca
    19021 cttcatcgaa agtcgcgcgt gtaattgctg tttgttcaat tgccatctca tgatcaccct
    19081 gttacgcata aacaaatgtg aaattataat cacaaaatat gcataaaaaa tcactaaatg
    19141 gcaatcagaa atcagcgatg caggaaatta gccagcagtt gatgtccttg ttcgctaaga
    19201 atactttctg gatggaactg cacaccttcc agatcccact ggcgatggcg aatccccata
    19261 atctcgcggg tttcgctcca ggccgtcacg tcaaagcacg ctggtaatga gtcaggttcc
    19321 accaccagcg aatggtagcg tgtcacggta agtggatttg ccagcccccg aaatacgccc
    19381 tcaccgctat gtgtaatcgg cgaggttttg ccgtgcatga cctttgcggc gcgcacaact
    19441 ttgccgccaa atgcctgcgc cattgcctga tgaccgaggc agacgccaag aatcggcaag
    19501 cgcccggcat agtgacgaat aacgtcaagg gagatcccgg cttcatctgg cgtacagggg
    19561 ccaggtgaga tgacaatttt ttgtggttta agggcgtcga tatccgccag cgtcaacgca
    19621 tcgttgcgct taaccagcac atccgccccc agttcacaaa agtactggta gaggttccag
    19681 gtaaaagaat cgtagttatc tataagcagg atcatggcgg ctccggtacg aaagaaccgc
    19741 gctattttac tcagattccc cggctttgct taccactttg ctaaatatca tctgcagtgc
    19801 ggtgagatcc cccattgccc cactctgatt tgcctgattc caggcctctt tttcgatacc
    19861 ctgccagctc agttgatatc cggcatgaat cgccagttgc tcgaagaaga tccgctgtgc
    19921 cagaccactt cccacccgga agggatgcag cacgttgatt tcacaatagt aatgcgccag
    19981 ccgctcgacg aacttcgctt tctccaggcc aaccagataa ccttcttcct ccagatcctg
    20041 catcagggca ttgccctctt tttcgatata agcaaagtgg cagaacggcg tatcgccctg
    20101 ataaatatca acttcacgca gttgccctgc ccagtcgaaa atatcctggt acagctggcg
    20161 atggatagtg cgcaaatgcg gtaaaccgcg caccagcgga ccaagctcaa tggtcgcagc
    20221 acgcagcgcc gtcatttcgt aagcggcctg ttccagccgc tgctgctgac ggatgttcag
    20281 ccggttacgc atgatatcaa ggccaggata aagatacgga tcgcgccctt caccgaattt
    20341 atcgctcata gtgcctcctc agctcttcaa ggcgcgctaa agcctctgcg gcagtaagag
    20401 tgactaaagg catctcgacg ccttcaagac ggcgactggc ctggaaatta cgattacgct
    20461 gaagctccca gagacgggac ttttgcttat cggtgagttt cttcacttga tgcctccctg
    20521 aacgtgtcca tttgccacaa gtataagcgg caaatccggg ttacgccggg agagcagaag
    20581 cgcgggcaag attgcccgcg cgagaaatca ttacggcagg actttagcgg aaaggataac
    20641 taccggtttt gacggcacat tctggtacgg accaacatca tgagtcggca cctgggaaat
    20701 cttatcggca acgtccatgc ctttcaccac tttaccaaat accgcgtaac cgaaatcacg
    20761 ctgaccatgg tcaaggaagg cgttatcggc aacgttgata aagaactggc tggtggcgct
    20821 gtctttgtca gcggtacgcg ccatcgcgat ggtgccacgc gtgttgcgca ggccgttatc
    20881 ggcttcattt ttgattggcg ggtttggttt tttctgctgc atctgctcgg tgaaaccgcc
    20941 gccctgaatc ataaagccag gaatgacgcg gtgaaaggta gtgttgttat aaaaaccgct
    21001 gttcacataa tcgacaaagt tttgcacaga cactggcgct ttttgtttat ccagctccag
    21061 ttcgatgtta ccagctgagg ttgtcaacaa tacgtgcggg tcccctttcg ctgccattgc
    21121 tgcgggagaa agagcagaaa gagcgaaaac agcagccatc gccgccaggg tcgatttgaa
    21181 catgagattt ccttaacaaa cagagcagaa aaaaagcaag tgaagagatt ctaaatagcc
    21241 ttcaagaaca aggccatccc tttacctaat tttacgtatc tgaaatatat gtaacctgac
    21301 gatcactttt tattccgtga tcaaaatcac ctcttaaaat gcaatttagc aaccgattgc
    21361 aataaaacat ttaaacagat cacaaaatca cctaaaatcg cccgctcgcg gcatatcccg
    21421 ctatgccatt ttatttttga acgcaggcca atcatgacta acagcaatcg catcaagctc
    21481 acatggatta gctttctctc ctacgcactg actggtgcgt tggttattgt caccgggatg
    21541 gtgatgggaa atatcgccga ttatttcaat ctgcctgttt ccagtatgag taacaccttc
    21601 accttcctca acgccggcat tttaatctct atcttcctca acgcctggct gatggaaatc
    21661 gtcccgttga aaacgcagtt acgttttggc tttctcctga tggtgctggc ggttgccggt
    21721 ttgatgttca gccacagcct ggcgctgttc tcgacggcga tgttcattct cggggtggtc
    21781 agcggcatta ctatgtcgat tggtacattc ctgataacac aaatgtatga agggcgtcag
    21841 cgcggttccc gcctgttatt tactgactcc ttcttcagta tggctgggat gattttccca
    21901 atgatcgccg cgtttctact ggcgcgcagc attgagtggt actgggttta tgcctgcatc
    21961 gggctggtgt atgtcgctat ttttattctg accttcggct gtgagttccc ggcactgggc
    22021 aaacatgcac caaaaacgga tgctccggta gagaaagaaa agtgggggat cggcgtactg
    22081 tttctctctg ttgcggcgct gtgctacatc ctcggtcagt taggttttat ctcctgggtg
    22141 cctgagtatg ccaaaggcct gggcatgagc ctgaacgacg cgggcacgct ggtgagtaac
    22201 ttctggatgt catacatggt cggcatgtgg gcgttcagct ttattcttcg cttctttgat
    22261 ttgcaacgca ttctgaccgt actggctggt ctggctgcga ttctgatgta cgtctttaac
    22321 accggaacac cagcacatat ggcgtggtca attctcgctc tgggcttctt ctccagcgcg
    22381 atctatacca ccatcatcac cctgggttca cagcagacca aagtaccgtc gccaaaactg
    22441 gttaactttg tcctgacctg cggaaccatc ggtactatgt tgacctttgt ggttaccggc
    22501 ccgattgttg aacatagcgg tccgcaggcg gcactgctta cagcaaacgg tctgtacgct
    22561 gtcgtctttg tgatgtgctt cctgttaggt ttcgtcagcc gtcaccgtca gcataacacc
    22621 ctgacctctc attaattgct aatgccggac ggcactatcg tcgtccggcc ttttcctctc
    22681 ttcccccgct acgtgcatct atttctataa acccgttcat tttgtccatt ttttgcacaa
    22741 acatgaaatg tcagacaatt ccgtgactta agaaaattta tacaaatcag caacatacca
    22801 cttaaggagt atataaaggt gaatttgatt tacatcaata agcggggttg ctgaatcgtt
    22861 aaggtaggcg gtaatagaaa agaaatcgag gcaaaaatga gcaaagtcag actcgcaatt
    22921 atcggtaacg gtatggtcgg ccatcgcttt atcgaagatc ttcttgataa atctgatgcg
    22981 gccaactttg atattaccgt tttctgtgaa gaaccgcgca tcgcttatga ccgcgtacac
    23041 ctctcgtctt acttctctca ccacaccgcc gaagagctgt cgctggtgcg cgaaggcttc
    23101 tacgagaaac acggcatcaa agttctggtc ggcgaacgcg ctatcaccat caaccgtcag
    23161 gagaaggtga ttcactccag cgccggacgt accgtttttt atgacaagct gatcatggca
    23221 accggttcct acccgtggat cccaccaatc aaaggttctg atactcagga ctgctttgtc
    23281 tatcgcacca ttgaagacct caacgccatt gaatcctgcg cccgtcgcag caaacgcggt
    23341 gccgttgttg gtggcggcct gttaggtctg gaagccgctg gcgcgctgaa aaacttaggt
    23401 attgaaaccc acgttatcga atttgcccct atgctgatgg cggaacagct tgatcagatg
    23461 ggtggcgagc agctgcgtcg caaaatcgaa agtatgggcg tgcgcgttca caccagcaaa
    23521 aacacccttg agattgtgca ggaaggtgtc gaagcgcgta aaaccatgcg ttttgccgac
    23581 ggcagcgaac tggaagtcga ctttatcgtc ttctctaccg gtatccgtcc gcgcgataag
    23641 ctggcaaccc agtgtggtct ggacgttgct ccgcgtgggg gtatcgtcat taatgattcc
    23701 tgccagactt ccgatccgga tatctacgcc atcggtgaat gcgcaagctg gaacaaccgt
    23761 gtatttggtc tggtagcacc tggctacaaa atggcgcagg tcgccgttga ccatattctc
    23821 ggtagcgaaa acgcctttga aggtgctgac cttagcgcca agctgaaact gctgggcgta
    23881 gacgtaggcg gtattggtga tgcgcacggt cgcacgcctg gcgcacgtag ctacgtttac
    23941 ctcgacgaaa gtaaagagat ctacaaacgc ctgattgtca gcgaagacaa caaaaccctg
    24001 ctcggtgcgg tactggtggg cgataccagc gactacggta acctgctgca actggtgctg
    24061 aacgctatcg aactgccgga aaacccggat tccctgatcc tgccagcaca ctctggtagc
    24121 ggcaagccgt ctatcggtgt tgataaactg ccggacagcg cgcaaatctg ctcctgcttc
    24181 gacgtcacta aaggtgatct gattgctgcc atcaacaaag gctgccacac agttgcggcg
    24241 ctgaaagctg aaaccaaagc gggtactggc tgcggtggct gtatcccgct ggtcactcag
    24301 gtactgaacg cggaactggc gaaacagggc atcgaagtta acaacaacct gtgcgaacac
    24361 tttgcttatt cgcgtcagga actgttccat ttgatccgcg ttgaaggcat taaaaccttc
    24421 gaagaactgc tggcgaaaca cggcaaaggc tacggttgtg aagtttgtaa accaaccgtc
    24481 ggttcgctgc tggcctcctg ctggaacgaa tacattctga agccggaaca tactccgctg
    24541 caggattcta acgacaactt cctcgctaac atccagaaag acggcaccta ctcggtgatc
    24601 ccgcgttctc cgggcggtga aatcaccccg gaagggctga tggcagtggg tcgtatcgcg
    24661 cgtgaattta atctctacac caaaatcacc ggctcccagc gcctggcgat gtttggcgca
    24721 cagaaagacg atctgccgga aatctggcgt cagctgattg aagctggctt cgaaaccggt
    24781 catgcctatg cgaaagcact gcgtatggcg aaaacctgcg tgggtagcac ctggtgccgc
    24841 tacggtgttg gcgatagcgt cggcctcggc gtggaactgg aaaaccgcta caaaggcatc
    24901 cgtacgccgc acaaaatgaa gttcggtgtc tccggctgta cccgtgaatg ttcagaagct
    24961 cagggtaaag acgtgggtat tatcgccact gaaaaaggct ggaacctgta tgtttgcggt
    25021 aacggcggca tgaaaccgcg tcatgcggat ctgctggcag cggatatcga tcgcgaaacg
    25081 ctgatcaaat atctcgaccg cttcatgatg ttctacatcc gtaccgccga caaactgacg
    25141 cgtaccgcgc cgtggttaga aaacctcgaa ggcggcatcg attacctgaa agcggtgatc
    25201 attgacgaca aactggggct gaacgctcat ctggaagaag agatggcgcg cctgcgtgaa
    25261 gcggtagtgt gtgagtggac tgaaacggtc aatacgccgt ctgcgcagac tcgcttcaaa
    25321 cacttcatca acagcgacaa gcgtgacccg aacgtgcaga tggtgccaga gcgcgaacag
    25381 caccgtccgg caacgccgta tgaacgtatc ccggtaactc tggtggagga caacgcatga
    25441 gccagtggaa agacatctgc aaaatcgatg acatcctgcc tgaaaccggc gtctgcgcgc
    25501 tgttaggtga cgagcaggta gcgattttcc gcccgtatca cagcgaccag gtgtttgcga
    25561 tcagcaacat tgatccgttc ttcgagtcca gcgtgctatc acgcgggctg attgcggaac
    25621 accagggcga gctgtgggtc gccagcccgc tgaaaaaaca gcgttttcgc ttaagtgatg
    25681 gcttgtgcat ggaagacgaa cagttttccg tcaaacatta cgaagcgcga gtgaaagacg
    25741 gcgtggtgca gctgcgcggt taatgtttta acgggaggcg caatgcctcc cctttttgca
    25801 tggtcctgta atgaccttcg gtatattgca gaacattttt taaacttttt gttttatttt
    25861 ttgtttttat tttttaaagg ataatcaaat gtttacagac actattaata agtgtgcggc
    25921 taatgctgcg cgcattgcac gcctgtcggc aaataacccg ctcggctttt gggtcagctc
    25981 cgccatggcg ggcgcatatg tgggtcttgg gatcatcctg attttcacgc tcggtaattt
    26041 gctcgacccg tccgtacgtc cgctggtgat gggcgcgacc tttggtatcg ccttaacgct
    26101 ggtgattatc gccggttctg aactgttcac cggacacgcc atgttcctca cctttggggt
    26161 aaaagcgggc agcatcagcc acgggcaaat gtgggcaatc ctgccgcaaa cctggctggg
    26221 taacctggtc ggttccgtct tcgttgccat gctctatagc tggggcggcg gtagcctgct
    26281 gccggtagat accagcatcg ttcactccgt cgcgctggct aaaaccactg caccggcaat
    26341 ggtactgttc ttcaaaggtg cattgtgtaa ctggctggtt tgcctggcta tctggatggc
    26401 gctgcgcact gaaggggcgg cgaaatttat cgctatctgg tggtgtctgc tggcatttat
    26461 cgcgtccggc tacgagcact ccatcgctaa catgacgctg ttcgccctct cctggttcgg
    26521 caaccacagc gaagcctaca cgctggcggg tattggtcat aacctgctgt gggtgacgct
    26581 gggtaatact ttatcgggtg ccgtattcat gggattgggt tattggtatg ctacgccgaa
    26641 agcgaatcgt ccggttgcgg acaaatttaa tcaaactgaa acggctgccg gttaattact
    26701 aaggggtttt tacgtggatc atttgcctat attttgccaa ttacgcgatc gcgactgtct
    26761 gattgtcggc ggtggtgatg tcgcggaacg caaagcaagg ttgctgttag acgcaggcgc
    26821 tcgcttaacg gtgaatgcat tagcgtttat tccacagttc accgcatggg cagatgcagg
    26881 catgttaacc ctcgtcgaag ggccatttga tgaaagcctt ctcgacacct gctggctggc
    26941 gattgcagcg acggatgatg acacgcttaa ccagcgcgtc agcgaagccg ctgaagctcg
    27001 tcgcatcttc tgtaacgtag tcgatgcgcc gaaagccgcc agctttatta tgccgtcgat
    27061 tattgaccgc tcaccgctaa tggtagcggt ctcctctggc ggcacctctc cggttctggc
    27121 gcgcctgttg cgcgaaaaac tcgaatcact gctgccgttg catctgggcc aggtggcgaa
    27181 atatgccggg caattacgcg ggcgagtgaa acaacagttc gccacgatga gtgagcgtcg
    27241 ccgtttctgg gagaaattgt tcgttaacga ccgcctggcg cagtcgctgg caaacaacga
    27301 tcagaaagcc attactgaaa cgaccgaaca gttaatcaac gaaccgctcg accatcgcgg
    27361 tgaagtggtg ctggttggtg caggtccggg cgatgccggg ctgctgacgc tgaaaggact
    27421 gcaacaaatt cagcaggcag atgtggtggt ctacgaccgt ctggtttctg acgatattat
    27481 gaatctggta cgccgcgatg ctgatcgcgt tttcgtcggc aaacgcgcgg gataccactg
    27541 tgtaccgcag gaagagatta accagatcct gctgcgggaa gcgcaaaaag gcaaacgcgt
    27601 ggtgcggctg aaaggtggcg atccgtttat ttttggccgt ggtggcgaag agctggaaac
    27661 actgtgcaac gcgggtattc cgttctcggt ggttccgggt attaccgcag cttctggttg
    27721 ctctgcctat tcgggtattc cactcacgca tcgcgattat gcccagagcg tacgcttaat
    27781 taccggacac ttaaaaaccg gtggcgagct ggactgggaa aacctggcgg cagaaaaaca
    27841 gacgctggtg ttctatatgg gattgaatca ggccgcgact attcagcaaa agctgattga
    27901 acacggtatg cctggcgaaa tgccggtggc aattgtcgaa aacggaacgg cagtcacgca
    27961 gcgcgtgatt gacggtacgc tcacgcaact cggtgaactt gctcagcaaa tgaacagtcc
    28021 atcgctaatt attattggtc gggttgttgg cctgcgcgat aaactgaact ggttctccaa
    28081 ccattaattt aacccgggcc agagaattct ggccttctta atgactcttt ttatatattc
    28141 agcaaataaa acataacccc ttaataaata ttatctgcta atgggttata ttcaccgtta
    28201 cagcgactta caaaattaag tcattcgctc actgacgcca attaaatatt cctgtatcgt
    28261 tct
//
LOCUS       DADMKF010000043        28041 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.044, whole genome shotgun sequence.
ACCESSION   DADMKF010000043 DADMKF010000000
VERSION     DADMKF010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28041
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.044"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(<1..133)
                     /locus_tag="JNC24_001672"
     CDS             complement(<1..133)
                     /locus_tag="JNC24_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAY9158436.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALARQNENHE
                     "
     gene            141..572
                     /gene="tnpA"
                     /locus_tag="JNC24_001673"
                     /pseudo
     CDS             141..572
                     /gene="tnpA"
                     /locus_tag="JNC24_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            632..706
                     /locus_tag="JNC24_001674"
                     /pseudo
     CDS             632..706
                     /locus_tag="JNC24_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709516.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
     gene            complement(703..2026)
                     /locus_tag="JNC24_001675"
                     /pseudo
     CDS             complement(703..2026)
                     /locus_tag="JNC24_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
     gene            complement(2026..3642)
                     /locus_tag="JNC24_001676"
     CDS             complement(2026..3642)
                     /locus_tag="JNC24_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAY9158437.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQTGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGPFILGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            3938..4654
                     /locus_tag="JNC24_001677"
     CDS             3938..4654
                     /locus_tag="JNC24_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAY9158438.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLTPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEYQVEYHLRRLHPEKS"
     gene            complement(4651..6312)
                     /locus_tag="JNC24_001678"
     CDS             complement(4651..6312)
                     /locus_tag="JNC24_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HAY9158439.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKDTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGLLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(6508..6936)
                     /gene="ibpB"
                     /locus_tag="JNC24_001679"
     CDS             complement(6508..6936)
                     /gene="ibpB"
                     /locus_tag="JNC24_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpB"
                     /protein_id="HAY9158440.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(7048..7461)
                     /gene="ibpA"
                     /locus_tag="JNC24_001680"
     CDS             complement(7048..7461)
                     /gene="ibpA"
                     /locus_tag="JNC24_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpA"
                     /protein_id="HAY9158441.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     gene            7767..8099
                     /locus_tag="JNC24_001681"
     CDS             7767..8099
                     /locus_tag="JNC24_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HAY9158442.1"
                     /translation="MIRNVLLAFMICSGMALLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(8101..9351)
                     /locus_tag="JNC24_001682"
     CDS             complement(8101..9351)
                     /locus_tag="JNC24_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HAY9158443.1"
                     /translation="MMAGPVLYQDRAMKQITFAPRNHLLTNTNTWTPDSQWLVFDVRP
                     SGASFTGETIERVNIHTGEVEVIYCASQGAHVGVVTVHPKSEKYVFIHGPENPDETWH
                     YDFHHRRGVIAEGGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGERVSFTYNDHVM
                     HELDPALDLRNVGVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAY
                     EEGWVGNHALAFIGDTLSPKGEKVPELFIVELPQDEAGWKAAGDAPLSGTETTLPAPP
                     RGVVQRRLTFTHHRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGE
                     PRQLTHNKTDIQSAFNWHPSGEWLGFVLDNRIACAHAQSGEVEYLTENHANPPSADAV
                     VFSPDGQWLAWMEGGQLWITETDR"
     gene            9416..10480
                     /gene="cbrA"
                     /locus_tag="JNC24_001683"
     CDS             9416..10480
                     /gene="cbrA"
                     /locus_tag="JNC24_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance protein"
                     /protein_id="HAY9158444.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQHGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNAITDCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSADILRSVLLKQPEKLNTAYWRATRKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGVAHIPQLKD"
     gene            complement(10477..11814)
                     /locus_tag="JNC24_001684"
     CDS             complement(10477..11814)
                     /locus_tag="JNC24_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY9158445.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASTLWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(11889..13037)
                     /gene="dgoD"
                     /locus_tag="JNC24_001685"
                     /pseudo
     CDS             complement(11889..13037)
                     /gene="dgoD"
                     /locus_tag="JNC24_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
     gene            complement(13034..13651)
                     /locus_tag="JNC24_001686"
     CDS             complement(13034..13651)
                     /locus_tag="JNC24_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HAY9158446.1"
                     /translation="MQWQTKLPLIAILRGIKPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKAVLPSDIAVFAVGGVTPE
                     NLAQWIDAGCAGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            complement(13635..14513)
                     /locus_tag="JNC24_001687"
     CDS             complement(13635..14513)
                     /locus_tag="JNC24_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HAY9158447.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLESRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPAHFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARALAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFTAGLERGLNTPAILPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVAHQHAITLVAGTSLTARYQQAFQAMGCDVTAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            complement(14510..15199)
                     /gene="dgoR"
                     /locus_tag="JNC24_001688"
     CDS             complement(14510..15199)
                     /gene="dgoR"
                     /locus_tag="JNC24_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HAY9158448.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            15168..15800
                     /locus_tag="JNC24_001689"
                     /pseudo
     CDS             15168..15800
                     /locus_tag="JNC24_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312658.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(15846..16658)
                     /gene="yidA"
                     /locus_tag="JNC24_001690"
     CDS             complement(15846..16658)
                     /gene="yidA"
                     /locus_tag="JNC24_001690"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HAY9158449.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(16773..17180)
                     /locus_tag="JNC24_001691"
     CDS             complement(16773..17180)
                     /locus_tag="JNC24_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HAY9158450.1"
                     /translation="MIIMGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQS
                     GGLGAILSTWLSNQQGNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPK
                     IIDALSPQGEVSPQANNDLLSAGMELLKGKLFR"
     gene            complement(17411..19825)
                     /gene="gyrB"
                     /locus_tag="JNC24_001692"
     CDS             complement(17411..19825)
                     /gene="gyrB"
                     /locus_tag="JNC24_001692"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HAY9158451.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(19854..20927)
                     /gene="recF"
                     /locus_tag="JNC24_001693"
     CDS             complement(19854..20927)
                     /gene="recF"
                     /locus_tag="JNC24_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HAY9158452.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMADTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(20927..22027)
                     /gene="dnaN"
                     /locus_tag="JNC24_001694"
     CDS             complement(20927..22027)
                     /gene="dnaN"
                     /locus_tag="JNC24_001694"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HAY9158453.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNPLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(22032..23435)
                     /gene="dnaA"
                     /locus_tag="JNC24_001695"
     CDS             complement(22032..23435)
                     /gene="dnaA"
                     /locus_tag="JNC24_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HAY9158454.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            23732..23812
                     /locus_tag="JNC24_001696"
     CDS             23732..23812
                     /locus_tag="JNC24_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9158455.1"
                     /translation="MTIDKNWLNRSNKDLGRSLRFTHQPV"
     gene            24042..24182
                     /gene="rpmH"
                     /locus_tag="JNC24_001697"
     CDS             24042..24182
                     /gene="rpmH"
                     /locus_tag="JNC24_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HAY9158456.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            24199..24558
                     /gene="rnpA"
                     /locus_tag="JNC24_001698"
     CDS             24199..24558
                     /gene="rnpA"
                     /locus_tag="JNC24_001698"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HAY9158457.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            24522..24779
                     /gene="yidD"
                     /locus_tag="JNC24_001699"
     CDS             24522..24779
                     /gene="yidD"
                     /locus_tag="JNC24_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HAY9158458.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            24782..26428
                     /gene="yidC"
                     /locus_tag="JNC24_001700"
     CDS             24782..26428
                     /gene="yidC"
                     /locus_tag="JNC24_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAY9158459.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISTFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIAAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            26534..27898
                     /gene="mnmE"
                     /locus_tag="JNC24_001701"
     CDS             26534..27898
                     /gene="mnmE"
                     /locus_tag="JNC24_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HAY9158460.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGLKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVIADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVEVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
BASE COUNT         6750 a         7582 c         7145 g         6564 t
ORIGIN      
        1 cctcatgatt ctcattctga cgtgccagag cacgattgaa aacgaaacga catgcgcctg
       61 cgaagcgcct catttcacgc tcctgttgac caccgggtct taactggaat ttaaatgctt
      121 gtagtcgttt cacaggccaa atgatacttt ggtctgtgaa aaaagaaacc gatatccggc
      181 gtggaaggca ttgtgttttc ctgatgcatg ttcacctggt ctttgtcacc aaataccgac
      241 gtcagatttt tgactatgac gcgacagaaa aactacgcac ttacttttca aatgtatgtg
      301 ctgattttga agctgaactg gtttaaatgg atggcgaacc agatcacgtc catttgttaa
      361 ttaactatcc tcccaaactg gcgatatcca gtctggtaaa cagcctcaaa ggcgtatcgg
      421 gtaggttact gcgacgagat cgaccagata ttgcagtcag gtattactac aaaggcgttt
      481 tgtggagtcc tggctatttt gccaatagct gcggaggtgc gccaatatcc gtcatccgcc
      541 aatacattga acaacagcaa acacctggtt aggtggaaaa ccgcgcctta tatccccgcc
      601 ctgaaggacg gggttttacg gcgcaccgga taaatttggt tatgcgccgt cagccgtgct
      661 aaagaatacg gaccagcacc caacggatgc cagtccacac aattaacgta actctggcca
      721 gtacgctttg ttggcctcca gcaactcatc cagaatagct ttggcgaccg aggcgctcgg
      781 taccgttttc gataacgtaa tcgcctgcca caggtgctga tatgaacgct gttcccaggc
      841 gtccacgacc agtttttcca ccgccacttg ctgactcatt aaccctttct gaaactgcgg
      901 gatatcgccg accaccagtg gttctggtcc gttgtgcccg accagacacg ggatctcgac
      961 catcgcttca tcatcaaagt tatggattgc cccgttgtta ggcacaatca gcaacatccg
     1021 ctcctgagtg ttgaaggcaa ttgccgccgc cagatcgacg atgtatgacg catgttcgtc
     1081 aatctccagc ttggccgccg ctgaatttcc tgccgctgta atggcgcggc aagcatcgaa
     1141 aacctgtttc tcgcgatgtt ccattcacct cattcgcgcg ggtatgttca gggttggaat
     1201 gctgaacaac ataatccggg aagagataat atttcagata ggtgttcggc aatgtatccg
     1261 ggtccagtgc ctggacatcc cgcgcttttg cataggtgtc gttccagctc gcttcagtat
     1321 gtgtatcttg ctgcggtgga acataaccgt gcttcgagac atattgccgc agctggggca
     1381 tcaggtcgtt gccctgcaaa tcttcaattg atgtccacca gccaaagtgg ttcaggccgt
     1441 agtagcgcac gcgcatctgt ttgcgatctt gcagcccaac aatttgcgcc atccggcttt
     1501 caataccgat tggcatgtca cagatgttga ggattttcgc attcgggcgc agacgacgcg
     1561 tggcttctgc gacaatgact gccgggttgg agtagttgag catccaggca tttggtgaat
     1621 atttttccat ataatccacc agttccagga cgccgccaat ggaacgcatg ccgtacgcta
     1681 ttccgccggg tccgcaagtt tcctgaccaa caacaccgtg gcgcagtggg attttttcat
     1741 ccagttcgcg catcgggtac ttgccgacgc ggatgtgcgc catgacaaaa tcaacgtcgc
     1801 tgaacgccac ttcaggatcg gtggtgtaac taaacgcaat gtccggcgct ttctctttaa
     1861 ggatgacttt acaggcttcg gcaatcactt cctgccgcgc gccatcgtta tcataaaatt
     1921 tcagtgcacg aagcgggaaa cgctcctgat tcgccaggag catcaacacg atgcctggcg
     1981 taaaggtgct tccaccgcct gcgacaacca ctgagaattt ggtcattata ctgcctccgt
     2041 aatggcaaca ttttctgctg attgatgaga attaattaag ctatcgagct gttcacgcag
     2101 ctgggataca tgcagaccaa tgatcacctg aatggcatcg ccactacgga agacgccgtg
     2161 cgctcccagc tttttaaaga cttcgtcatc cagcgtttgt gacatgtcat gcagtgcaat
     2221 gcgtagacgc gtcgcacaat tgttaatgct ggagatattg ccgaccccgc ccagggcttg
     2281 caggataccg gcagcctgat ccagctcttt ttttggctct gccgcggtgg tttggcctcg
     2341 cgaggctttg tattcggctt ttgagtagag tttcacttcc gcatcttcac gtcccggcgt
     2401 gcacatgttg aactgcagaa tcagggtgcg gaaaaccacg aagtacagca gggtaaagca
     2461 caacccaatg gcgatttggg tcagcatcat atccgcgtgg ttgctgaaca tcgggatcca
     2521 gttttgcggt aaaacctggt caatcagacc tccgcccatg ttgcccacca caccaaagag
     2581 atacatcacg gtcgacattg aggccgcaag cacagcgtgt accgcaaaca gcaacggtga
     2641 aatgaacagg aaggtaaatt ccagcggttc ggtaattccc accagcatgg cggttaaggt
     2701 tgcgggaatc agcaagcccg ccacttttac ccgattttcc ggtgcggcag tgaagtacat
     2761 cgctaaagaa atgcccacgg caccaaagat ttttgagtta ccgtgcaggg caaaaccgcc
     2821 ttccgggaac aacgatttca gcggctcggc gctcaaactg aactcttgca gatgctgcgc
     2881 ccagtacatc tgaatgccgc cttcaacagc tgccggaccc aagataaacg gtccgtagat
     2941 gaagtggtgt aaaccggttg ggatcagaat acgttcgagg aaggtgtaaa cccagacccc
     3001 aagcgcaccc gccgaacgca ggaacgcttg cagagattca atccccgttt gtacttttgg
     3061 ccagccgagc aatgtcagcc aggcacaggg gatcatcacc aggaaggcga taatcaccac
     3121 ataagacgtc ccctggaaaa tgccgagaaa aaccggcagt tttttatcga acagacggtt
     3181 atgcagcgcc gtcacaatgc cggaaatgat aattgcgccg ataatgctgg tatcgagggt
     3241 tttaatcccg gccatcattg tcagaccgct acctgccacc gcgtcctgag tgaaatcgac
     3301 gccgaagtag cttccccagg tcattcccat cgcgttgatg aaatagttcc aggtcaggaa
     3361 actcaccatc accgccagac aagcacgtcc ctgcgcttgc ttagcaaggc caatgggtaa
     3421 accgacagca aaaatcagcg gcatattacg gaataccgtc caaccgccct cttcaataat
     3481 gtgtacgatt tgcgcgaata aactgttcgg atcggtcagt gattccccga caaacatcgg
     3541 gttttgcagc aagatggcaa gacccaccac aatcccggcg aagggaaaca gcagcactgg
     3601 cgtaaacatc gcgccgccaa agcgttgaat ttgactgagc atttccacat cctcttttct
     3661 caattctgta agtaggaagc ctgattggtt atgcggcagg cctggagcaa ggataggaag
     3721 atgcgatcgg cagaacatga cgacccaatg ggattcgtga tcgctttcat gcttttcgtt
     3781 gggcggtaga gatctacgtt ttcgcgaaaa agtagacatc tgtgagcaca ggtacacagc
     3841 atcgaacaca tctttaaaaa aaagatgttt tttcaatcga ttaagcagaa cttgtgtgcg
     3901 cattacccgg gcttgcaggc aaaaaagaga tctagagatg atctacaaaa gcattgcgga
     3961 gcggttaaga attcgactta actccgcaga tttcacgcta aacagccttc ttccgggtga
     4021 aaaaaagctg gcggaagagt ttgcggtatc gcggatgacc atccgtaaag ccattgacct
     4081 gctggtagcg tgggggctgg tggtccgccg ccacggtagt ggcacttacc tggtgcgcaa
     4141 agatgtactg catcaaaccg ccagtctgac cgggctggtg gaggtgttaa aacggcaggg
     4201 aaaaactgtc accagccagg tgctgatttt tgaaatcatg cctgcgcctc cggccattgc
     4261 cagccagtta cggattcaaa tcaacgagca gatctacttc tcccgtcgcg ttcgttttgt
     4321 ggaagggaaa ccgctgatgc tggaagacag ctatatgccg gtaaaactgt tccgtaatct
     4381 ttcgctgcaa catctggaag ggtcgaagtt tgaatatatt gaacaagagt gcgggatttt
     4441 gattggcggt aattatgaaa gcctgacgcc ggtgctcgcc gatagactgc tggcgcggca
     4501 aatgaaggta gcggaacaca cgccactgct gcggatcacc tcgttgtcat atagcgagag
     4561 cggggagttt ttgaattatt cagtgatgtt cagaaatgcc agcgaatacc aggtggagta
     4621 ccatttacgg cgactccacc cggaaaagag ttaaccgata ctccagaaga gcaccgccag
     4681 taattggggg gtgataatgc gcaggaacat cactaacgga tagacagtgg cgtaagagag
     4741 cgccgccgca ccgctagttg gatgaagatt attagcaaac gccagcgccg gaggatcggt
     4801 catggaacct gccagcatcc cgcacatggt caggtaattc attttggcta acatccgcgc
     4861 cagaatgcca acagtaatca gcggaacggc ggtgatcagg gcaccataac caatccagct
     4921 tagcccttcg ccattgacca gggtattcac aaaatcccca ccagatttca gaccaacgac
     4981 cgagaggaac agcacgatcc ccagctcccg cagcgcgagg ttggcacttg gcggcataaa
     5041 ccagtacagc ttgccgatac tgccgatacg cccgaggatc aacgccataa tcagcagacc
     5101 gcccgccagc cccagtttca acgcggccgg gaatcccggc acaaagacgg gaatagaacc
     5161 taacaatacg ccaagcccga tgccaataaa cactggcagc atctgaacct gttgcagttt
     5221 ttgctgcgca ttccccagca cattggcaac ggcatcaatt gcggacggac gccccaccag
     5281 gttgaggata tcgccgaact gcaggctgat atcgccactg gcgaccagtt cgaccccggc
     5341 acggttcagg cgcgagataa caacgtcata gcgttcttta aagtgcaggt cgcgaatacg
     5401 ttttccgagc acgttttcat tggtgaccac cacacgctcg acgcgcaaat cagtgtcttt
     5461 cgtggacagc gaagtatcga cctcctgacc aatcaccagt tgcgcattat gtaaatccgc
     5521 tggctgaccc accagatgca gcaaatcgcc cagttggata atggtatctg gcgaaggaac
     5581 ttttagggtt tcttcgcgtt tcagacgcga gcagataatt ttgtcgccgt tgagaatcgg
     5641 tacatcttta atggctaaat catgcaggtt agggttctca acgcgaatat tgatagtctt
     5701 gatcagcgcg ccgccattgg tgcgtgaaga ctcgtgctgc tgagcttctg tctcgacatt
     5761 gacgcggaaa ataacccgca acatccacat ggtgaacaaa atcccgcaaa tgccgaatgg
     5821 atacgccatc gcgtaactca tccccatctg atcgaccatt tccattggtg tacccaggtc
     5881 gcgcaaaatc tgctgccctg cccccagcgc tggcgtattg gtaaccgcac cggagaaaat
     5941 ccccagcact accggcagtg gaatatcaaa cagtttatgc aggatggcgg taaccagacc
     6001 accgatgatg acgatcagaa cagcaaacag gttgaggcgt aatccggaga cgcgcaatga
     6061 ggcaaagaag cccggcccta cctgaatccc gatggtataa acgaacagga tcaggccaaa
     6121 ttcctgaata acatgcagca tatcgctact taatgtcatc cccgcctgag aaacaaaatg
     6181 gccgacgatg atcccaccaa acagcacgcc gccaatacct aatcctatgc cgcgaaattt
     6241 gacgttgccg ataaacaaac cgacgactgc caccaaagcc agaatactga ccgttaatgc
     6301 tatatcactc atcgtccatt tctcttgaga taacatttta attgctacgg attatggctg
     6361 aatcggtgaa gcagatttgc cttatagcgc acattattac gggaaagaac gtgccgaaat
     6421 atcttaaaca gtcgaaattt aacgcattgt tgaagaagaa agacaaaaaa agccccacca
     6481 gaatggcggg gcaaagagaa tagctagtta gctatttaac gcgggacgtt cgctgatagc
     6541 gatacgctgc gctgcgatgg gttcaggctc attacgaatt aaatcaatat gcagtaaacc
     6601 gtttacgaaa gttgcgccag agacttccat attttcagcc agcgtaaagc tcaggctaaa
     6661 tggctgattc ataagccctt gatgcagcca ttttttctct tcttttggct gctccggcgt
     6721 gccttttacg ctcaggcgcg taccttccag ttgaatctct aaatcttcct gacggaaacc
     6781 tgccagcgca agggtaatgc ggtagtggtt atcgtcgctt ttctcaatgt tgtacggcgg
     6841 gaagctctga ctttcaccgg cgttttgcag tgcgttggcc agtttgtcaa aaccgatcca
     6901 ttgacgcatc agtggggata aatcgaagtt acgcatagtc atttctcctt ctaagaagcg
     6961 agtaagtacc tgcaaatccg aagattcgca tatgctccct gacggcgagc atggagatgt
     7021 caggccgcgc caggcggcct taaggaatta gttgatttcg atacggcgcg gttttttcgc
     7081 ttccggaatc acgcgttcga gatcgatata cagcaaacca tttaccaggt tagcgccacg
     7141 aacatgaatg ttctcagcta actggaattt gcgttcaaag ttgcgttcag cgatgccctg
     7201 gtacagatag gtgcgctctt tttgttcgtc ggcgtgagca cctttcacca ccagcagatt
     7261 atcctgggcg gtaatttcca gttcgctctc agcaaaacca gccacagcga tagcaatgcg
     7321 gtaatggttt tcgtctacca gttcaacgtt atacggaggg tagccgccat tactctggct
     7381 ctggttgttt tctaagtggt taaacaaacg gtcaaatcca atagcagaac ggtaaagcgg
     7441 ggataaatca aagttacgca taatcaatag ctcctgaaat cagcgagaat gtaagacctt
     7501 ccacaatgga caggtcaggt agccagaaca cccatcaggc gcgtcctcat cgactacgat
     7561 gtaaaaatgg gtctggaaat gaacttttca agccttatca gcggactttt ttttgcagtt
     7621 tatggtctat tgcatagact gagggggcag cacgaaacgt taaagtgtga tagccgccac
     7681 agagcgacgt aatgatgatg ttattttcag caagggatcg ctataactca tcaggccaac
     7741 acccacgatc aacagatgaa tttattatga taagaaatgt gttgttagcg ttcatgatat
     7801 gcagcggaat ggcattactc ggaggatgct ccagcgtgat gtcccatacc ggagggaaag
     7861 aaggaacgta tcccggcacc cgcgccagcg ccaccatgat tggcgatgat gaaaccaact
     7921 ggggcactaa gtcgctggcg attctcgata tgccgtttac cgctgtgatg gacacgcttt
     7981 tattaccgtg ggatgtgttc cgcaaagaca gctcggtgcg ctcacgcgtt gaaaaaagcg
     8041 aagccaacgc gcaagccacc aatgcggtta ttccaccggc cagaatgcct gataactgat
     8101 ttagcgatca gtttcggtga tccacagctg gccaccttcc atccacgcca gccattgacc
     8161 atccggcgag aagaccacgg cgtccgcaga aggtggattg gcgtggtttt cggttaaata
     8221 ctcaacctcg ccgctttgcg catgggcaca agcaattcga ttatccagca caaagcccaa
     8281 ccattctcct gacggatgcc agttaaatgc agactgaata tccgttttgt tatgggttaa
     8341 ctggcgcggc tcgccgccct gtggcgagat aagccacagt tgcacaatgc cgttatcatc
     8401 acgcattaaa aacgcgattt gcgtaccctg cggattacag cgcacccagt ggcgcgggac
     8461 gttgactaac cccggataag cccgatggtg ggtaaaggtt aaacgtcgct gcacgacgcc
     8521 acgcggtggc gcgggcaggg tggtttccgt tccacttaac ggcgcatcac ctgccgcttt
     8581 ccagccagct tcatcttgcg gcaactcaac gataaacagc tccggcactt tctcaccctt
     8641 tggcgaaagt gtgtcgccaa taaacgccag cgcgtgattt cctacccatc cttcttcata
     8701 agcacgattg atttcatcac tgccaggctg cggcgtgggc gtggttttac tcaccagcac
     8761 acaccagtgg ctgccgctgt attcacgcgg atgctgcttt tgtacgttga ccggaccaaa
     8821 cggcgcagca acaccgacgt ttcgcaaatc cagcgccgga tcgagttcat gcattacatg
     8881 gtcgttatag gtaaagctca ccctttcacc gttcgggcta aagacatgca catggctgcc
     8941 gccgcgcagc gctcctggcg tatacggcgc ggtaatatcc attgcatcga gattgctcac
     9001 cttgccgcct tcagcaatga ctccgcgccg atgatggaag tcgtaatgcc atgtttcatc
     9061 aggattttcc gggccgtgaa taaagacata tttctctgac ttcggatgaa cggtcaccac
     9121 gccgacgtgt gcgccctgtg acgcacagta gataacctcg acctcaccgg tatggatatt
     9181 cacacgctca atggtctcgc cggtaaacga cgccccagaa ggacgcacgt caaataccag
     9241 ccactggctg tcgggcgtcc aggtattggt attggtgagc aggtgattac ggggagcaaa
     9301 ggttatctgt ttcatggcgc ggtcctgata caaaacgggt ccagccatca tacttcacaa
     9361 ggacttcact ttcagacaat agtcttttgc catcgtgatg aatgtgagtg aaaagatgga
     9421 acatttcgac gtggcgatta ttggcctcgg cccggcaggg tcggcgttgg cacgaaagtt
     9481 agccggcaaa atgcaggtga tcgcgctgga taaaaagcac cagcatggta ctgaaggttt
     9541 cagcaaacct tgtggcggtc tgctggcacc ggacgcgcag cgatctttta ttcgcgatgg
     9601 gctgacgctt cctgtcgatg tgatcgctaa tccgcagatt ttcagcgtca aaaccgtcga
     9661 cgtcgccgca tcgctcacgc gtaactacca gcgaagctat atcaatatta atcgccatgc
     9721 tttcgacttg tggatgaaat cgctgatccc cgccagcgtt gaggtttatc acgacagcct
     9781 gtgtcgaaaa atctggcgtg aggatgataa atggcatgtc atttttcgtg cagacggctg
     9841 ggagcagcat attaccgccc gctatctggt cggtgccgat ggtgccaact cgatggtgcg
     9901 gcgacatctc tacccggatc atcaaatccg taaatatgtc gcaatccagc agtggttcgc
     9961 agagaaacat ccggtgccgt tctactcctg catctttgat aatgcgataa ctgactgtta
    10021 ttcatggagt atcagcaaag acggttattt tatctttggc ggtgcctatc caatgaaaga
    10081 cggtcagacg cgtttcacga ctctgaaaga gaaaatgagc gcctttcagt tccagtttgg
    10141 caaagcggtg aaaagcgaaa aatgcacggt gctgtttccc tcacgctggc aggattttgt
    10201 ctgcggtaag gacaacgcct ttctgattgg tgaagcggcg ggatttatca gcgccagctc
    10261 gctggagggg attagctatg cgctggatag cgcagacatt ctgcgttcgg tgttactgaa
    10321 gcagccagag aagctcaata ccgcttactg gcgcgccacc cgcaaactgc gtttaaaact
    10381 cttcggcaag atagtaaaaa gccgatgcct gaccgcaccg gctttaagaa agtggattat
    10441 gcgcagtggt gtggcacata ttccacagtt gaaggattag ccaacgcgct tcacatcgcc
    10501 caccagcagg atataagaga gcgcgccaat caacgcgacg gcggagatat aaaccagtgc
    10561 aggtgcgaaa ccgtaaccct gcgccaggta ccccaccacc agcggaacgg tgatgccgcc
    10621 cagaccaccg gcgaagttaa acacgccgcc ggttaaacca atcaggcgca tcggtgccag
    10681 agaagaaacc agcgaccagg taattgaagc aaaaccgtta ccgaagaatg ccagcgccat
    10741 caggcacata atcatcatcg gatcgttagt gtagttagcg cccataatgc aggtggagat
    10801 cagcaagccg cagataatcg gcgttttacg cgcaaagccc agtgaaaagc ccttacgtac
    10861 cagcagatcc gctacccagc cagagagcag gacgccgaca aacgccgcga ggaatggcac
    10921 cgtggtcata aagcctgctt tcagcgccgt gattcctttt tcctgggtta aatagttcgg
    10981 gaaccaggtt aagaaaaacc acagtgtaga agccaccgca aattgcccaa gatagacgcc
    11041 gatcagttta cgatggaaca ccagtttcca gtctttggct gttaacggct gacgtgcctc
    11101 tttcttcact ggcgcatcac catccaccag accgccgcca tcacgaatgt aatccagttc
    11161 agctttgctg atgcctttgg tcaggcgcgg cggctgataa accttaaacc aaatcagcga
    11221 ccagataatg ccgataccac cagtcacaat gaacacccag tgccagctca acatctcctg
    11281 aatccagatc agcagcggag tcaaaaacgc cagaccgaca aactgaccag acgtataaaa
    11341 accaacggcg gaggcgcgtt catgttccgg gaaccagctg gtcaccatcc ggttattggt
    11401 cgggaaagcg ggcgcttcga aaataccggt tatcgcacgc agaccaatta atgacattaa
    11461 tcccgtggca aagccctgga ataaagtcgc cactgaccag ccaaatatcg caataaaata
    11521 agtcacgcga gaacctacgc gatctaaaaa ccaaccgccg gggatctggc atagcgtata
    11581 aagccaggcg aaggccgaaa atacatagcc catttccgct ttcgtaatgc cgaactcttc
    11641 ctgaatatgg gcggaagcca cggccaggtt agcgcggtca acataacaaa tgaccaccgt
    11701 aataaagatc atcaccagcg tcagataacg ccgacgcccc ggctttgctg cattaacggg
    11761 aatatccata gcgagctttc tccagatttt gggcatagcg aagccgctca ccatgccctg
    11821 taatttacag agggttatat ttttgtattg ctgttttagt gcccgatgag gggcttacgt
    11881 ggcaggaatt accactctgc tacgctgtta tcttcatgac gccagagtgg attacgccag
    11941 tccggggcat ttttactgaa ctcaataact ttagcttcgt cgatttccac gcctaagccc
    12001 ggtttcgtta gcggtttaaa gaagccgcca accatgctga agtcttcttt gtttttcaca
    12061 aagtcgagta actccgcgcc tttgttgtaa tgaattccca tactttgttc ctgaagtacc
    12121 gcgttatagg aaacaaagtc gatatgcaga caagccgcca gtgcaatcgg tccgagcgga
    12181 cagtgcggcg caagggtcac gtcataggct tctgccattc cggcaatttt gtagcattcg
    12241 gtaataccgc ctgcatggga gagatccggt tgtagaatcg aaataccgcc tgcctccagc
    12301 acgcgtttaa aatcgaaacg tgagaacatg cgctcacccg ccgccagtgg aatatgcgtt
    12361 tgtgccgcca atttcgggta gtattcggct tgttccgcca gcaccggctc ttcaataaac
    12421 agcgggcgat acggctccag ttctttaatc agcactttcg ccattggcgc gctgacgcga
    12481 ccgtggaaat caagaccaaa ctcaatctga ttgccaaaag cttcacgaat ttgtgccacg
    12541 gtgttgactg ccgcatctac cgcgcgggag ttatcaatta gccccagttc ttcacaaccg
    12601 ttcagtttga aggtatcgaa gccgatttcg cgcagggttt taataccgtc gatcacatcc
    12661 gccggacgat cgccgccgac ccaactgtag gctttaattt tgtcgcgaac caggccgccc
    12721 atcagttgcc agaccgacgc attcagcact ttacctttga tatcccataa cgcctggtcg
    12781 atcccggcaa tggcgctcat caggattgga ccgccacgat aaaatccggc gcgatacatc
    12841 acttgccata agtcattgat gcgctatgga tcctgaccaa tcaaatagtc acccagctcg
    12901 tgaactgccg cttccaccgt gcgggcgcgg ccttcaatca ctggctcgcc ccagccgacg
    12961 acgccttcat cggtttcaat tttcaggaac atccagcggg gaggtaaacg atacgtggta
    13021 attttggtga ttttcattgc actgcctctc gatacgcctt aacaaatgct gctgcctgct
    13081 gcgcggtgcg ttctacggac tgcccggcgc gatagagatc gctgcctaag cccgccccag
    13141 cacaacctgc gtctatccac tgcgccaggt tttctggcgt cacgccgcca acggcaaaga
    13201 ctgcgatgtc cgatggcaat accgctttta acgctttgat gtattgcgga ccaaaagccg
    13261 atgacggaaa tattttcagc gcctgcgcgc ccgcttcgag cgcggtaaag gcttcggtcg
    13321 ccgtcgcgca gccggggcag acggtcatgc cgtagcccac cgcacggcgg atcacttcac
    13381 tatggatatt gggcgtaacg atgagctgac agcccatcct ggcgagcgca tcgacctgtt
    13441 caggtttcag taccgtacct gcgccaatca acgccttgtc gccatacgca tcaacgatgg
    13501 cgggaatgct ttgctcccat tgtggggaat tcagcgggat ttcaaccgcg tcgaacccgg
    13561 cgtcaatcac cgcgccaaca tgcgccagcg cctcgtcggg cttaatgccg cgcaaaatgg
    13621 cgatcagcgg aagtttagtt tgccactgca tgagcgatgc tccttatacc agcctgaaat
    13681 gccgtgtcgc ccgccaccgc cgtcacgtcg caacccatcg cctgaaaggc ttgctggtag
    13741 cgcgcggtca acgatgttcc ggcgacaagg gtgatggcgt gttgatgggc cacatagtcg
    13801 cgcatactgg cgacctctgc gccaatcaac aaaccagaga gaaattcact gacctgttca
    13861 cggggaagtg ttcccagcac atgcgaggca cgaacttcaa aaagctgcgg caatatggcg
    13921 ggcgtattaa gaccacgctc aaggccagcc gtgaaggcat cggcagagtt ttcctgcggc
    13981 ggcaaacctg caccaatcaa tgagtgattt aacagtaaat gatgtaattc accggtcatc
    14041 acggtgcgaa aatcgttgat ttgctggcta tcggcctgca cccatttgca atgcgtaccg
    14101 ggcatgacat aaagagagga aggagccaga gctcgcgcgc cgatcaattg agtttcttcg
    14161 ccgcgcatca cattgtggtt atcatcatga gagacgcata atccgggaat aatccagata
    14221 ttgtcgccaa cagacgttaa ttgttcgcca atagacgaaa aatgggcagg aacagataaa
    14281 tacggagcaa ctttccagcc gacattgctg ccgaccattc ctgccattac cactggcgta
    14341 ttctcttcac gccagtcggt cgtgacttct gctaacaccg cagccggaga ttttccgttc
    14401 aggcgcgtga cgcctgcttc tgattgcctg ctctccaggc agtggtcgcc ctgataaagc
    14461 caggcgcgca gattggtcga tccccagtca attgcgatgt agcgagctgt catgtgattt
    14521 cctttaacct tcgtgtcgag ctggcgatca tggtaagcgc cgcctgctct gccgcatcgc
    14581 cgtcctgatg ccgtatcgca tcgaacagcg ccttatgttc ctggagcgtt tgcggcatgt
    14641 tggcctcatc gcccatccag gttcgttcaa aaaccgcccg ctgcagcgaa ctgatcgcaa
    14701 tgctaagttg ctgtaacacc gggttatgca ccgactgcag caccgcctcg tggtagcgaa
    14761 tatccgcttc gttaaacgct tcgcggtcct gattgttggc aatcatctcg ttcagcgccg
    14821 attcaatctg cgccagatcg ctggaggtcg cgcgctctgc tgcccaacgg gcaatcgccg
    14881 gttccaccag atttcgcact tcgctcatgg cactgataag ccgtgggtcg tagtcgtttt
    14941 ccagcaccca ttgcagtacg tcagtgtcga ggtaattcca ctggttacgc ggtgccacaa
    15001 acgcgccgcg ataacgtttc atttcaatca gccgtttcgc catcaacgaa cggaacacct
    15061 cacggatgat gttgcgcgag gttgcaaact cctcacagag ttccgcctca gccggaagcg
    15121 gcgagcctgg tacgtatttg ccgtgaacga tctgtttacc cagcgtaatg acgatgcgat
    15181 cggttttatt gagagtcatg gagagtcctt gtgctctgta tgttcttctc tactttaccc
    15241 cgatcgatgc ataatcagga aagtatcaat cgcagtcaga caccatcacg gggctatcgc
    15301 aggcaaagga tagtaatgga acaaaaggct atgtttttgt aggggaatcg ctggattacc
    15361 ttatcacaga tggtgccgat gacatcgtta agatgctcaa ggatccagct cttaaccggc
    15421 acaatattca ggttgctgat gacgcaagat ttgttttaaa tgcggggaaa aagaaattta
    15481 ccggcacaat atcactctat tacaactgga gcacagaaga agaaaaagca cgagtgacac
    15541 aatatcgttt tgcttgtggt gttcagcact gtacccggac agtagaaaac ctggaaggta
    15601 ctatccatga gaaaaataaa aacatggatt actcaaacgt aatggctttc taccatccat
    15661 ttaaagtgcg attttatgaa taccattcac ccagaggcat tccggatggt gtttccgcag
    15721 cattactgcc agtgactgtt acgctggaca tcattactgc accgctgcaa tttctggttg
    15781 tatatgcagt aaaccaataa tcagtaagcg ggcaaacgcg tttatgctgt ttgcccgccc
    15841 acagattaat tcagcacata cttctcaata gcaaacgcca cgccatcttc aaggttagat
    15901 ttggtgacaa agttcgccac ttctttcacc gaaggaatag cgttatccat cgccacaccg
    15961 acgcctgcat attcaatcat tgcgatgtcg ttttcctgat cgccaatcgc catgatttct
    16021 tccggtttaa tacctaacac gtcggccagt gatttcacac ccgtaccttt gttaacgcgt
    16081 ttatcgagga tttcgaggaa gtacggcgca cttttcagca cggtatattt ctctttcact
    16141 tcctgcggaa tacgcgcgat agcctggtcg aggatggcgg gttcatcaat catcatcact
    16201 ttcaggaact gggtattggg gtccattttc tccgcttcgc agaacaccag cggaatagtg
    16261 gcaacgaaag attcatgcac tgtgtagtag ctgatatcgc ggttggcggt gtacagcgtg
    16321 gtgcggtcca gggcgtggaa atgagaaccg acttcgcgag agagtttttc caggaaacga
    16381 tagtcgtcat agctgagagc agtttgcgcc acggtgctac catcagctgc cttctgtact
    16441 agcgcgccgt tataagtaat gcagtagtcg cccggctgtt ccatatgcag ctctttcagg
    16501 tagttgtgca cacctgcata cgggcgaccc gtcgttagca cgacattcac gccacgggcg
    16561 cgagctgcgg caatcgcatt tttaacggcg ggtgaaatgg tgtgatcggg cagcagaagg
    16621 gtgccatcca tatcgatagc aatgagtttg atagccatga gttccccagg tagattggtt
    16681 cctgacccat gctaacgcga ttccgctcaa aaatcagtac aacacccgag ggaaaagggg
    16741 gatgcaacgc gggttgcatg ctccccattt gcttagcgga agagtttccc tttcagcagt
    16801 tccatacctg cggaaagcag atcgttattg gcttgtggtg acacttcacc ttgcggtgag
    16861 agcgcatcaa taatcttcgg caattgctct gccagtaaac tggaagctgt actggtatcc
    16921 acgccaagtt tttgcccgag atcggacacc gcatttgtgc cgagcgccga ttccagttgc
    16981 tcgccactaa ccgattgatt gccctgttga ttactcagcc aggttgagag aatggcccct
    17041 aagccgccac tttgcagttt ttccagcagc acctgaatgc cgccctgctc ctcaacccaa
    17101 cttaaaatag cctgatattt ccccgcatcg cctttcagaa aggcaccgac aacttcatca
    17161 aaaagcccca tgataatcac ctgtaaagcg ttacgtgttg acccaaaaag tatagatttg
    17221 cgggatgata attgcggatt gcagaaataa aaagggcgga gatgatctcc gcccttttct
    17281 tatagcttct tgccggatgc ggcgtgaacg ccttatccgg cctacaaaat catgaaaatt
    17341 caatacattg caagattttc gtaggcctga taagcgtagc gcatcaggca cgctcgcatg
    17401 gttagcgcca ttaaatatcg atattcgccg ctttcagggc gttctcttca ataaacgcac
    17461 ggcgcggttc aacggcgtcg cccatcagcg tggtgaacaa ctggtcggca gcaatcgcat
    17521 ctttaacggt aacgcgcagc atacgacggc tttccgggtc catggtggtt tcccacagct
    17581 gttccgggtt catctcgccc agacctttat aacgctggat ggagaggccg cgacgggact
    17641 ctttcaccag ccagtccagc gcctgctcga agctggctac cggctgacga cgctcgccac
    17701 gttcgataaa cgcatcttct tccagcaagc cacgcagttt ctcacccagc gtgcagatac
    17761 gacgatattc gccaccggtg ataaactcgt gatccagcgg atagtcagta tccacaccgt
    17821 gggtacgcac gcgaacaatc ggttcaaaca ggttttgttc ggcattggtg tggacatcaa
    17881 acttccactg gctgccgtgc tgttctttgt cgttcagttc gctgaccagc gcgttcaccc
    17941 agcgggtaac ggtctgctca tcagagaggt cggcttccgt cagcgtcggc tgatagataa
    18001 gctctttcag cattgctttc ggataacgac gctccatgcg gttgatcatt ttctgcgtcg
    18061 cgttgtactc agacaccaat ttctctaacg cttcgccagc caatgccggt gcgctggcgt
    18121 tggtgtgcag cgttgcgcca tccagcgcga tagagatctg gtactgatcc atcgcttcgt
    18181 cgtctttaat gtactgttct tgcttgcctt tcttcacttt gtacagcggc ggctgagcga
    18241 tgtagacgtg accgcgttca acgatttccg gcatctgacg atagaaaaag gtcaacagca
    18301 gcgtacgaat gtgcgagccg tcgacgtccg catcggtcat gatgatgatg ctgtgataac
    18361 gcagtttgtc cgggttgtac tcgtcacgac cgataccaca gccaagcgcg gtgataagcg
    18421 tcgccacttc ctgagaagag agcatcttat cgaagcgcgc tttctcaacg ttgaggattt
    18481 tacccttcag cggcagaatc gcctggttct tgcggttacg cccctgcttc gcagagccgc
    18541 ccgcggagtc cccttccacc aggtacagtt cggaaagcgc cggatcgcgt tcctggcagt
    18601 ctgccagttt gcccggcagg cccgctaagt cgagcgcacc tttacggcgg gtcatttcac
    18661 gcgcgcgacg cgccgcttca cgggcacggg cagcatcgat aattttgcca accacgattt
    18721 tcgcgtcggt tgggttttcc agcaggtatt ctgccagcag ttcgttcatc tgctgttcaa
    18781 ccgctgattt cacctcagaa gaaaccagtt tgtctttggt ctgggaggag aatttcgggt
    18841 ccggcacttt cacggaaacg accgcaatca ggccttcacg cgcatcgtca ccggtggcgc
    18901 taactttggc ttttttgctg tagccttctt tgtccatgta ggcgttcagg gtacgggtca
    18961 tcgccgcacg gaagcctgcc aggtgagtac cgccgtcacg ctgcggaatg ttgttggtaa
    19021 agcagtagat gttttcctgg aagccatcgt tccactgcaa cgccacttcg acgccaatac
    19081 cgtctttttc agtggagaag tagaagatat tcgggtggat cggcgttttg ttcttgttca
    19141 gatattcaac gaacgccttg atgccgcctt catagtggaa gtggtcttct ttgccgtcgc
    19201 gcttgtcgcg cagacgaatg gaaacgccgg agttgaggaa cgacaactca cgcagacgtt
    19261 tcgccagaat ttcatattcg aactcggtca cattggtgaa ggtttcgagg ctgggccaga
    19321 aacgcaccat ggtgccggtt ttttcagtct cgccggtaac cgccaacggt gcctgcggta
    19381 caccgtgttc gtagatctga cggtgaattt taccctcgcg ctggataacc agctccagtt
    19441 tttgcgacag ggcgtttact accgaaacac caacgccgtg cagaccgccg gacactttat
    19501 aggagttatc gtcaaattta ccgcctgcgt gcagaacggt catgatcact tccgccgccg
    19561 atacgccctc ttccgggtga ataccggtcg gaatgccgcg cccgtcatcc tgtacagaga
    19621 cagagttatc ggcgtgaatg gtgacgataa tttctttaca gtgacccgcg agcgcttcgt
    19681 cgatagcgtt atctaccacc tcgaatacca tgtggtgcag accggtgccg tcatccgtgt
    19741 cgccgatata catacccggg cgcttacgca ccgcatccag ccctttcagg actttgatac
    19801 tggaggagtc ataagaattc gacatcaacg tttctcgctc atttatactt gggttaatcc
    19861 gttattttac ccttttccac ggtaaacatc ttcgaatttt cgtccgacat gtctataacg
    19921 tgttcagcac tgatcgcgct gacaaagacc tgtgattgcg tcgcttttaa gcggctggca
    19981 agcagcccgc gacgctcatc atcaagctca gaggcaaaat catctatcag gtagagacac
    20041 cgccgcccgc tttcacgggt gaggaactct ccttgcgcca gacgtaaggc gcacatcaac
    20101 agcttaagct gcccacgcga taaggtatct tccaccggcg caccgtcggc gcgaatgcgt
    20161 aagtccgctt tatgcgggcc atgcgcggtg taggttagct ggcgatcgcg ttcaaaatta
    20221 cgttccagca cctcagcata ttctgtctct ttctcccagc cgcgctggaa agagaaagtc
    20281 agagaaaact cagggagaaa ttgcttacaa gtatcggcca tatcagccgc gataccggcg
    20341 ctatactccg cgcgccaggt gctgatttgc tccgccagcg ggatcagctc tttatcccac
    20401 gggcgtagct gttcgtaacg tgtcacctgg cgcagcgccg cattgcgctg cttgagcaat
    20461 cgcttgagat tgctccaggc ggtgaaaaat ccgggttcgt tgtgaaagca tccccagtcg
    20521 aggaatgctc ttctgtattt ggggccgccg ttgagtaaag taaacccttc tggcgttatc
    20581 aactgcattg gcatcaggtg cgccagttcc gcgaccttat gcccgtctgt accgtcgatg
    20641 cggactttgc tgtcgccctg tttgtctttg gttaagccaa tcgctgtctc gcgctcttcg
    20701 ccctgtaatc gcccgtggag aacaaacgcc tcctgctcat ggcgaatgac gcgaccaatc
    20761 tgcaaactgc gaaacgcccg accatggccg agcgtataga tggcttccag cacgctggtt
    20821 ttgccactgc cgttggcacc taccagaaag ttaaagccgg gagataaagc gagatccgcg
    20881 gtttcaatgt tgcggaaatc gcggatcaac aagcgggtga gggacattac agtctcattg
    20941 gcatgacaac ataggcagcc gactgtgatg cggcatcttc aatctgcacg ctggaaaccg
    21001 aatcggtcag catcatgcgg acgttttcac atttcagcgc gttcagaaca tccagcacgt
    21061 agctgacgtt gaagccgatt tccatctccg caccgctata ggtaacgtcg aggatctctt
    21121 ccgcttcttc ctgttccggg ttgttggcgg tgattttcag ctggttttcg ctgacataaa
    21181 gacgtacgcc gcggaatttc tcgttagaga gaatcgccgc gcgagcaaac gcctgcttga
    21241 gcagatcgca gccagcttcc agatgtttgt ccgggttctt cggcagaacg cggcgataat
    21301 ccgggaagcg accatccacc agtttggagg tgaagataaa gtcgccaacg tgggcgcgaa
    21361 tgttgttgct gccaatctgt acgcgcagcg gattgtcgcc gccgtcgagc atacgcatca
    21421 gttcaatcac gcctttacgc ggcacgatca ccgaatggct tggcaaagat tgaccaattg
    21481 gcattgaaca gactgccaga cggtggccgt cggtcgccac ggtgcgcagt tcttcgcctt
    21541 cggtttcaaa cagcataccg tttaagtaat agcgaacgtc ctgatgcgcc atagaaaact
    21601 gggtcgcttc aatcagacgc ttcatcgtcg cctgcggcag ggtaaattcg acttcgctct
    21661 gccagtcatc gaggttcggg aaatccgccg ctggcagggt agacagcgaa aaacggctac
    21721 gcccggagcg caccagcatc cgttcacctt ccagctgtac ggcaatttcc gcgccttcag
    21781 gcagaccacg gcagatatca aagaatttgc gcgccggaac ggtcgtcgct cctggctcgt
    21841 gtggctgaac cagcgcaacg cgtgccacca tttccatctc gagatcagta ccggtcagcg
    21901 acaacgtacc gtcagcaacc tgtaacagca gattaccgag aatcggtagc gtaggacgac
    21961 cacctaacgg cccgctcacc tgttgtagcg gttttaataa atgctcacgt tctacggtaa
    22021 atttcatagg tttacgatga caatgttctg attaaatttg aaaaatcttc tttgatatcg
    22081 tggctctctt cacgcaactg ctcgatctta cggcaggcat gaagcaccgt cgtgtggtca
    22141 cgaccaccaa acgcatcgcc aatctccggc agactgtggt tagtcagctc tttcgccagc
    22201 gccatcgcca tctggcgcgg acgcgccacc gagcgggatc gacgcttgga aaggagatcc
    22261 gcgactttga tcttgtagta ctccgccacc gtcttctgaa tattgtcgat ggtgaccagt
    22321 ttttcctgca atgccagcaa gtcgcgcagt gcctcacgca cgaagtcgat ggtgatcgcc
    22381 cgtccggtaa agttggcgtt agcaataacg cggttcagcg ccccttccag ctcacgtacg
    22441 ttagatcgta gacgcttggc gataaagaac gccacttcgc ctggcaaacg aatgtcattt
    22501 tcgtcggcct ttttcatcag gatcgccacg cgggtttcca gctctggcgg ttcgatcgcc
    22561 acagtcagtc cccaaccgaa gcgggatttc aaacgatcct caacgccgtt gatctctttc
    22621 ggatagcgat ccgaggtgag aatgatctgt tgattacctt ccagcagggc gttgaaggtg
    22681 tggaaaaact cttcctgaga tcgttcttta ttagcaaaaa actgaatatc gtcgatcagc
    22741 agtgcatcta cggaacggta gtagcgttta aactcttcga tcgcgttgtt ttgcagggct
    22801 ttaaccatgt cctgaacaaa gcgctcggag tgcatataaa ccactttggc attcggcttg
    22861 cgcgccataa tgccgttacc caccgcatgc agcaggtgag ttttacccag acccgtgccg
    22921 ccataaagga acaatgggtt ataggcaccg ccagggttat ccgccacctg gcgagccgcc
    22981 gcgcgcgcca gttggttaga tttaccttca acgaagttat caaacgtgtg tttgacgttt
    23041 acgttagaac gataggtcgg ttctgccggg gccgggacgt tatcccaacc tgagcgcgta
    23101 gaaggcgcag cacgttgcgg ctgcgtttgc gccacctgtg caggggccgc gacgttgctc
    23161 gtcactgccg cttgtggcgt ttgcgtcacc ggtttggtgc cgacttcaaa acgcagctgt
    23221 ggggcatccg ctccgcagaa actggttagc agtccattga tattattaag gtacttgtcc
    23281 cgtacccaat cgaggacaaa acggtttggc gcgtacaggg ccagcgtgtt atcgctcagt
    23341 tccgcctgca atgggcgtat ccacatactg aattctgtgg ctggtaactc atcctgcaat
    23401 cgggcaagac actgctgcca aagcgaaagt gacacggcgg actccactcg aacaaaagtc
    23461 gataatgact aaggctgaaa cattcatgat tgttgacgta cgtcgaaaag accctgcttg
    23521 agggtgacgc acgaaccgct gtctgcggtt atatgcccga tcaagatcct gcaaaacgat
    23581 cgggaccgcg gatcatagcc taaactgcgc aagagatctt ctgtttctca cagattttcc
    23641 cgatttatcc acaggacttt ccagacctcg ctaagtgtaa acgatcctgc cgcgaggcgg
    23701 gcacgattta cgccgcatat tggaaaattt aatgaccata gacaaaaatt ggcttaatcg
    23761 atctaataaa gatctaggac gatccttgcg ctttacccat cagcccgtat aatcctccac
    23821 ccggcgcgcc atgctggttt tcactggtgt gaggtcgtac attttccctg cgaaaaggtg
    23881 cggaaaagcg cggtaaataa ggaaagagaa ttgactccgg agtgtacaat tattacaatc
    23941 cggcctcttt aatcacccat ggcttcggtg tccatcgttt catttttcgg cggatatcca
    24001 ataaagccat tgaatttatt caagtttagg tagaaatcgc catgaaacgc acttttcaac
    24061 cgtctgtact gaagcgcaac cgttctcacg gcttccgtgc tcgtatggct actaaaaatg
    24121 gtcgtcaggt tctggcacgt cgtcgtgcta aaggccgcgc tcgtctgacc gtttctaagt
    24181 aataaagcta acccctgagt ggttaagctc gcatttccca gggagttacg cttgttaact
    24241 cccagtcaat tcacattcgt cttccagcag ccacaacggg ctggcacgcc gcaaattacc
    24301 attctcggcc gcctgaattc gctggggcat ccccgtatcg gtcttacagt cgccaagaaa
    24361 aacgttcgac gcgcccatga acgcaatcgg attaaacgtc tgacgcgtga aagcttccgt
    24421 ctgcgccaac atgaactccc ggctatggat ttcgtggtgg tggcgaaaaa aggggttgcc
    24481 gacctcgata accgtgctct ctcggaagcg ttggaaaaat tatggcgccg ccactgtcgc
    24541 ctggctcgcg ggtcctgata gccctcattc gggtctatca acgcctgatt agtccgctac
    24601 tcgggccgca ttgtcgtttc actccaacct gttcaagcta cggaattgag gcattgcgca
    24661 ggtttggagt gataaaaggc agttggttga cggtgaaacg cgtattaaaa tgccaccctt
    24721 tacaccctgg tggtgacgat cccgtcccgc ccggaccatt tgataccaga gaacactaac
    24781 gatggattcg caacgcaatc ttttagtcat cgctttgctg ttcgtgtctt tcatgatctg
    24841 gcaagcctgg gagcaggata aaaacccgca acctcaggcc caacagacca cgcagacaac
    24901 gaccaccgca gcgggtagcg ccgccgacca gggcgtaccg gccagtggcc aggggaaact
    24961 gatctcggtt aagaccgacg tgcttgatct gaccatcaac acccgtggtg gtgatgttga
    25021 gcaagctctg ctgcctgctt acccgaaaga gctgaactct acccagccgt tccagctgct
    25081 ggaaacttca ccgcagttta tttatcaggc acagagcggt ctgaccggtc gtgatggccc
    25141 ggataacccg gctaacggcc cgcgtccgct gtataacgtt gaaaaagacg cttatgtgct
    25201 ggctgaaggt caaaacgaac tgcaggtgcc gatgacgtat accgacgcgg caggcaacac
    25261 gtttaccaaa acgtttgtcc tgaaacgtgg tgattacgct gtcaacgtca actacaacgt
    25321 gcagaacgct ggcgagaaac cgctggaaat ctccaccttt ggtcagttga agcaatccat
    25381 cactctgcca ccgcatctcg ataccggaag cagcaacttc gcactgcaca ccttccgcgg
    25441 cgcggcgtac tccacgcctg acgagaagta cgagaaatac aagttcgata ccattgccga
    25501 taacgaaaac ctgaacatct cttcgaaagg tggttgggtg gcaatgctgc aacagtattt
    25561 cgcgacggcg tggatcccgc ataacgacgg taccaacaac ttctataccg ctaatctggg
    25621 taacggcatc gccgctatcg gctataaatc tcagccggta ctggttcagc ctggtcagac
    25681 tggcgcgatg aacagcaccc tgtgggttgg cccggaaatc caggacaaaa tggcagctgt
    25741 tgctccgcac ctggatctga ccgttgatta cggttggttg tggttcatct ctcagccgct
    25801 gttcaaactg ctgaaatgga tccatagctt tgtgggtaac tggggcttct ccattatcat
    25861 catcaccttt atcgttcgtg gcatcatgta cccgctgacc aaagcgcagt acacctccat
    25921 ggcgaagatg cgtatgctgc agccgaagat tcaggcaatg cgtgagcgtc tgggcgatga
    25981 caaacagcgt atcagccagg aaatgatggc gctgtacaaa gctgagaagg ttaacccgct
    26041 gggcggctgc ttcccgctgc tgatccagat gccaatcttc ctggcgttgt actacatgct
    26101 gatgggttcc gttgaactgc gtcaggcacc gtttgcactg tggatccacg acctgtcggc
    26161 acaggacccg tactacatcc tgccgatcct gatgggcgta acgatgttct tcattcagaa
    26221 gatgtcgccg accacagtga ccgacccgat gcagcagaag atcatgacct ttatgccggt
    26281 catcttcacc gtgttcttcc tgtggttccc gtcaggtctg gtgctgtact atatcgtcag
    26341 caacctggta accattattc agcagcagct gatttaccgt ggtctggaaa aacgtggcct
    26401 gcatagccgc gagaagaaaa aatcctgatt cggtgagttt tcgctaaaat aagggcggtc
    26461 agttgaccgc cttttttctt ttcgtagggt ggataagcac cgcgcatccg ccacacaaag
    26521 caacaggaac atcatgagcg ataatgacac tatcgtagcc caggccacgc ctccgggacg
    26581 tggcggcgtt ggcatcctgc gcatctccgg cctaaaagcc cgtgaagttg ccgaaaccgt
    26641 gctgggtaaa ctgcctaagc cgcgctacgc cgattatctt ccgtttaaag acgccgacgg
    26701 cagcgtgctc gatcagggga ttgcgctatg gttccctggc ccgaactcgt tcaccggcga
    26761 agatgtgctg gaactgcagg gtcatggcgg tccggtgatc ctcgacctgc tgttaaaacg
    26821 cattctgacc attcccggcc tgcggattgc tcgccctggt gagttttccg aacgcgcgtt
    26881 tcttaacgat aaactcgact tagcccaggc cgaggcgatt gccgatctta tcgacgccag
    26941 ttcggaacag gcggcccgtt cggcacttaa ctcgctgcaa ggcgcattct ccgcacgggt
    27001 caaccatctg gtggaagccc tcacccactt gcgcatttac gtcgaagcgg caattgattt
    27061 ccctgatgaa gagatcgatt tcctctccga cggcaaaatt gaagcccagc ttaatgacgt
    27121 gattgccgat ctcgatgcag tgcgtgctga agcacgtcag ggtagtttgt tgcgcgaagg
    27181 gatgaaggtg gtgattgccg gacgtcctaa cgccggtaaa tcgagcctgt taaacgcgct
    27241 ggcgggccgt gaagcagcaa tcgtaactga tatcgccgga accacacgtg acgtgctgcg
    27301 tgagcatatc cacattgacg gaatgccgct gcatatcatc gataccgctg ggctacgtga
    27361 agccagtgac gaagtggaac gtattggtat cgagcgcgcg tggcaggaaa ttgaacaggc
    27421 cgaccgcgtg ctgtttatgg tcgatggcac cacaacagac gccgtggatc cggcagagat
    27481 ctggccggaa tttatcgccc gtctgccagc gaaactaccg atcaccgtgg tgcgcaataa
    27541 agccgatatc accggcgaaa cgctgggaat gagtgaagtg aacggtcacg cgttaattcg
    27601 tctctcggca aggacaggtg aaggcgtgga ggtgctgcgt aaccatctca aacagagcat
    27661 gggctttgac accaacatgg aaggcggctt cctggcgcgt cgtcgccacc tacaggcgct
    27721 ggaacaggca gcggagcatt tgcagcaagg taaagcgcaa ctgttaggtg cctgggcggg
    27781 tgaactgctg gcggaagagt tgcgcctggc acagcagaac ttaagcgaaa tcaccgggga
    27841 atttacctct gatgatttgc tggggcggat tttctccagc ttctgtattg gtaagtaacc
    27901 gcgcttacga agccgtattc tgactgtcag atgcggcttc gcttcattgt tagcactcct
    27961 gttattcctc aacccttttt ttaaacatta aaattcttac gtaatttata atctttaaaa
    28021 aaagcattta atattgctcc c
//
LOCUS       DADMKF010000044        27784 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.045, whole genome shotgun sequence.
ACCESSION   DADMKF010000044 DADMKF010000000
VERSION     DADMKF010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27784
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.045"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            99..761
                     /gene="acrS"
                     /locus_tag="JNC24_001702"
     CDS             99..761
                     /gene="acrS"
                     /locus_tag="JNC24_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrS"
                     /protein_id="HAY9158461.1"
                     /translation="MAKRTKAEALKTRQELIETAIAQFAQHGVSKTTFNDIADAANVT
                     RGAIYWHFENKTQLFNEMWLQQPSLRELIQEHLTAGLEHDPFQQLREKLIVGLQYIAK
                     IPRQQALLKILYHKCEFNDGMLAEGVIREKMGFNPQTLREVLQACQQQGCVANNLDLD
                     VVMIIIDGAFSGIVQNWLMNMAGYDLYKQAPALVDNVLRMFMPDENITKLIHQTNELS
                     VM"
     gene            complement(764..943)
                     /locus_tag="JNC24_001703"
     CDS             complement(764..943)
                     /locus_tag="JNC24_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 domain-containing protein"
                     /protein_id="HAY9158462.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLTTETDKCRNMNSVYP
                     LKLVNCDELNFQDRM"
     gene            complement(1027..1911)
                     /gene="yhdJ"
                     /locus_tag="JNC24_001704"
     CDS             complement(1027..1911)
                     /gene="yhdJ"
                     /locus_tag="JNC24_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HAY9158463.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDALAELKKLPTESVDLIFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIVECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPEALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAIASGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            complement(1997..2293)
                     /gene="fis"
                     /locus_tag="JNC24_001705"
     CDS             complement(1997..2293)
                     /gene="fis"
                     /locus_tag="JNC24_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HAY9158464.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            complement(2319..3284)
                     /gene="dusB"
                     /locus_tag="JNC24_001706"
     CDS             complement(2319..3284)
                     /gene="dusB"
                     /locus_tag="JNC24_001706"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HAY9158465.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSDPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            complement(3613..4494)
                     /gene="prmA"
                     /locus_tag="JNC24_001707"
     CDS             complement(3613..4494)
                     /gene="prmA"
                     /locus_tag="JNC24_001707"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HAY9158466.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     IVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYVDSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(4506..5957)
                     /gene="panF"
                     /locus_tag="JNC24_001708"
     CDS             complement(4506..5957)
                     /gene="panF"
                     /locus_tag="JNC24_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HAY9158467.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(5947..6189)
                     /locus_tag="JNC24_001709"
     CDS             complement(5947..6189)
                     /locus_tag="JNC24_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HAY9158468.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVAAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(6298..7647)
                     /gene="accC"
                     /locus_tag="JNC24_001710"
     CDS             complement(6298..7647)
                     /gene="accC"
                     /locus_tag="JNC24_001710"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HAY9158469.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     RGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(7658..8128)
                     /gene="accB"
                     /locus_tag="JNC24_001711"
                     /gene_synonym="fabE"
     CDS             complement(7658..8128)
                     /gene="accB"
                     /locus_tag="JNC24_001711"
                     /gene_synonym="fabE"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HAY9158470.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(9106..10080)
                     /locus_tag="JNC24_001712"
     CDS             complement(9106..10080)
                     /locus_tag="JNC24_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAY9158471.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASRILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            10232..12172
                     /gene="csrD"
                     /locus_tag="JNC24_001713"
     CDS             10232..12172
                     /gene="csrD"
                     /locus_tag="JNC24_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HAY9158472.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSTLRPQITELMMSADIVRVDLLHGDKQVYTLARNGSYRPVGSSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIIVMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIAGQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTHVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            12477..13520
                     /locus_tag="JNC24_001714"
     CDS             12477..13520
                     /locus_tag="JNC24_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HAY9158473.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            13586..14689
                     /gene="mreC"
                     /locus_tag="JNC24_001715"
     CDS             13586..14689
                     /gene="mreC"
                     /locus_tag="JNC24_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HAY9158474.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGVSQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPTTGNAATA
                     PAAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            14689..15177
                     /gene="mreD"
                     /locus_tag="JNC24_001716"
     CDS             14689..15177
                     /gene="mreD"
                     /locus_tag="JNC24_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HAY9158475.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSTLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            15186..15779
                     /gene="yhdE"
                     /locus_tag="JNC24_001717"
     CDS             15186..15779
                     /gene="yhdE"
                     /locus_tag="JNC24_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HAY9158476.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADTIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            15769..17238
                     /gene="rng"
                     /locus_tag="JNC24_001718"
     CDS             15769..17238
                     /gene="rng"
                     /locus_tag="JNC24_001718"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HAY9158477.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            17306..21106
                     /locus_tag="JNC24_001719"
     CDS             17306..21106
                     /locus_tag="JNC24_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709041.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein"
                     /protein_id="HAY9158478.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWHYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSTLKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPEAVNEALSGSVAWDGKVGIDLPYHAGATYNVELNGDLNNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQYITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDITISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            21262..22707
                     /gene="tldD"
                     /locus_tag="JNC24_001720"
     CDS             21262..22707
                     /gene="tldD"
                     /locus_tag="JNC24_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HAY9158479.1"
                     /translation="MSLNLVSEQLLAANSLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(22835..23700)
                     /locus_tag="JNC24_001721"
                     /pseudo
     CDS             complement(22835..23700)
                     /locus_tag="JNC24_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            23883..24086
                     /locus_tag="JNC24_001722"
     CDS             23883..24086
                     /locus_tag="JNC24_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AaeX family protein"
                     /protein_id="HAY9158480.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            24094..25026
                     /gene="aaeA"
                     /locus_tag="JNC24_001723"
     CDS             24094..25026
                     /gene="aaeA"
                     /locus_tag="JNC24_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HAY9158481.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQILFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGGRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSNLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            25032..26999
                     /gene="aaeB"
                     /locus_tag="JNC24_001724"
     CDS             25032..26999
                     /gene="aaeB"
                     /locus_tag="JNC24_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HAY9158482.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVFAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            27091..27363
                     /locus_tag="JNC24_001725"
     CDS             27091..27363
                     /locus_tag="JNC24_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9158483.1"
                     /translation="MNIYTFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(27419..27682)
                     /gene="yhcN"
                     /locus_tag="JNC24_001726"
     CDS             complement(27419..27682)
                     /gene="yhcN"
                     /locus_tag="JNC24_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HAY9158484.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDTAQAQNREAIGTVSVSG
                     VASSPMDMREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
BASE COUNT         6679 a         6953 c         7368 g         6784 t
ORIGIN      
        1 aatgttaccc tctcgtataa atacacatta gatgatagat taaccttcgc tattttctca
       61 ccctgtgtcg aatatattta tttcctgaat aattaatcat ggcaaaaaga accaaagccg
      121 aagctctgaa gacccggcaa gaactgattg aaactgccat cgcccagttt gcgcaacatg
      181 gcgtaagcaa gacgacgttc aacgacattg ccgatgccgc taacgttacg cgtggcgcta
      241 tctactggca cttcgagaac aagacccaac tgtttaatga gatgtggttg caacagcctt
      301 cattgcggga gttaatccag gaacacttga cggctggatt agagcatgac ccgtttcaac
      361 aattgcgtga aaaattgatt gtcggcttgc aatatattgc caaaattccc cgccagcagg
      421 cgttgctgaa aatcttatat cacaaatgtg aatttaatga tgggatgctg gccgagggag
      481 tgatacgcga aaagatgggc tttaatccgc agactctccg cgaagtattg caggcgtgtc
      541 agcaacaagg ttgtgtagca aataacctcg atttagatgt tgtgatgatt attattgatg
      601 gtgccttcag cggaattgtt caaaactggt taatgaatat ggcgggttat gatctttata
      661 aacaagcacc cgcgctggtc gataacgtat taagaatgtt catgccagat gaaaacataa
      721 cgaaattaat tcatcaaaca aatgaattaa gtgtcatgta aattcacatt ctgtcctgaa
      781 aattcagttc gtcacagtta accagtttta gtggataaac tgagttcata ttacggcatt
      841 tgtctgtttc tgttgtcagc agttcaatcc actgcatcag taaagtatca aacagaaaaa
      901 cgaagacagc gaaaacgacg agccaccaat acttgcgaat cattacctga gcctgtgcat
      961 gtttaaccaa gtctggcagg aaatatacac gaaatgaata atctgaaaac caggcttaca
     1021 cttcctttac tttgcaatga gatcggggtc aacttcgctt aaccggcttc gttttgacag
     1081 gttgccggtc tttctttttt tcactttcgc cagttcttcc gcggagtaat gcgacgcgac
     1141 atccagccgt cgaagcccca ttttgatgta ctcgctgttg atctcaatac caatgaattt
     1201 tcgtccgctg gcgatggcta cggcaccggt agtaaagcta ccggcaaacg ggtcgagaac
     1261 gatatcgcct gggttggaag aggcgagaat aatgcgtttc agtaaggctt ccggtttttg
     1321 cgtcgggtgg ttctcatatt catccatcaa ataacgcacg cgcggaaaat cccagacgtt
     1381 acccggtact ttttgatgat tgtatggctg tggaggattt ttgcgataat cgatcaacgc
     1441 gcgctgcgat ccggttttgg cttctaccag aatagcatca ccgttgaatg tgtagttctt
     1501 tgcgtctttc accatcatca ggatgggttc gtacatggag ccgtagtgtt ttttcgcctg
     1561 tactccagaa ctgtcatatg accagacgat gcgacttttg atggtaaaaa gcttgcggca
     1621 ctggagatcg ataaagggca tgttttccgt actgttcata atgtacatgc tgccctgctt
     1681 tttcagaacg cggtggcact ctacaatcac ttcaaacagc cagtcgataa acagatcttc
     1741 tttccaggct tcgatcagac catcaaaatt tttaccgatg ttatacggtg ggtcggcaaa
     1801 gatcagatcg acgctttcag taggtagctt tttaagttcg gcaagggcat caccgtgaat
     1861 aatggtctta gcttcattac caaaccgggt cggttcacat cctgttctca tggtcactcc
     1921 ctttgtgaca cctataaaaa aggcgcttcc ccatgccgag tagcgccttt ttaatcaagc
     1981 atttagctaa cctgaattag ttcatgccgt attttttcaa ttttttacgc agcgtaccac
     2041 ggttgatgcc catcatcagc gcagcacggg tctggttacc acgggtgtat tgcatcacca
     2101 tgtccaacag gggctgttct acttcagcca gtaccagctc atagaggtca ttcacatcct
     2161 gaccattcag ttgagcaaaa tagttcttca gtgcctgttt aaccgagtca cgcaggggtt
     2221 tttgggttac ctgatcctga gagttaacgg tagaaacggt cagtacgtca gaatttacgc
     2281 gttgttcgaa catagttctg tcagctcttt atttctgttt acgcaaaatt ttcgaagtat
     2341 gcctccaacg cctccagctg ttcgctggca tcctcaatgg cgttgaatgt gcgccgaaac
     2401 tggtcatttg gagcgtgttc ctggagatac caggaaacgt gtttacgtgc aattcggtac
     2461 ccttttgccg gaccataaaa gtcatgcagt tcccgaacgt gcgcgcaaag caagcgctta
     2521 acctctgcca aaggcagcgg gggcagcaac tccccagtgt ccagataatg ctggatttcc
     2581 cgaaagatcc agggtcttcc ctgagctgcg cggcctatca tcagggcatc cgcccctgta
     2641 tagtcgagca cagctctggc tttaagcggg tcagtaatgt cgccattcgc gataaccgga
     2701 atggaaactt tctgcttaac tgcccgaata ctgtcgtact cagcttctcc attgaacaaa
     2761 caggcgcgtg tacggccatg aatggtcaga gcctgaatgc cacagtcttc agccagttgg
     2821 gcaatctctt cgcagttacg gtgttccggt gcccagccgg tgcgaatctt cagggtaaca
     2881 ggaacgtcca ctgcattgac gacctcggta aggatcgatt taacgacatc cgggtactgc
     2941 aagagggctg aacctgcgag cttgcgattc acttttttag ccgggcaacc catattgata
     3001 tcaataatct gggcaccgct ttccacgtta atacgtgctg catctgccat ttctttcgga
     3061 tcgctaccag caatttgcac ggtgcgaata ccgggttcat caatgtgcac catccgtaaa
     3121 cgagatttgt cgctttccca aacctgtggg ttagaagaca tcatctcgga tactgtcaat
     3181 ccggctccca tctcgtagca caacgtccga aaaggtctgt ctgtaatgcc agccatgggc
     3241 gctgcgatca ggcgatttct gagctgatat tgtccgatgc gcatgagtta agaaatgacc
     3301 atactgtgac tgcaaggcgg cgtatattac gcattttttg cacgagatga aaggccaaac
     3361 tttgaccaat cctctgagat ggatcaaaga attgcattta aaatgagcgt ggtgagataa
     3421 ttactcataa aaatcatcat attagaaaat agtgactaaa atttacactc aaagaaattt
     3481 gagtaagttc tcaatttttc tttatgaatg aaaattttgg cacgcaaatt tgcgtaaata
     3541 accggcaaat ttacgtgcct tttgtgagct tgctcgcact tcgccccgcg tcaccctacg
     3601 gcgatgcgaa ggttaattct tacgaccggt aatgcggcac cactcttctt tttccacgac
     3661 cgggtccagt gcgaagctgt cgacataagc ttcacaaacg ctctctgcct ggcttgccag
     3721 aataccggaa aggcccagca aaccgcctga aaccggcagg acgctgatta acggtgccag
     3781 ttcacgtaat gggcctgcaa ggatgttagc gaccaccacg tcggctttca tttcttctgg
     3841 ctgatctttc ggtaagtaga gttccagacg gtcagaaacg ccattacgtt cggcgttgtc
     3901 gcggctagcc tgaatcgcct gcggatcgat atcaatacca atggcttttg ctgcacccag
     3961 tttcagcgcc gcgatagcca gaatgccgga accacagcca aagtcgatga ctattttacc
     4021 ggttaaatcg aggctgtcga gccactgcag gcacagagag gtagttggat gggtacccgt
     4081 accaaacgcc agccctggat ctaacatcac gttgacggcg ttttcgtccg gcacatcacg
     4141 ccagctaggg cagatccaca gtcgttcacc aaagcgcatc gggtggaaat tatccatcca
     4201 ttcgcgctcc cagtctttat cttccagttg ttcgatttta tgcgcgaagc ctgcgccgag
     4261 cagcggatgg ttttccagaa tcgccaccac gtcgttcata tcggtttcag cgtcgaacag
     4321 accaatcaca tcggtgtcgc cccacaggcg cgtttcgccc ggcagcggct caaatactgg
     4381 cgtatcgtgg gtatcctgaa aagtgataga aacggcaccc gcttccatca gcgcatcgct
     4441 aagatcttca gcgttcgcgc cggtggtgtt cagtttcagt tggatccaag gcatggcaaa
     4501 actctttatt tatcagtagt caaaacggta gcttgcggga cggatgtacc gaaacggttt
     4561 ccgaccagga aagccagcaa acttagtagt aacgagggca cgatagggtg gaagcccagg
     4621 tactgaatat tcagcgtcgc gagtacggca tacagcacgc cgccaacgat catcgcactt
     4681 agcgcgcctt tggcgttggc gcgttcccag taaagaccca gcaccagcgg ccacaggaaa
     4741 acggcttcca gcccaccgaa ggccagcaaa ttcagccaga tgatcatttc tggcggcttc
     4801 caggcggcaa gcagcagcaa cgcgccgaga actaacgtaa ttaccgccga catccgcttc
     4861 agacgcgtct cgttttgcat ttgatccgga cggatattca gatagagatc tttaatgatc
     4921 gtagcggaac tttgtagcaa ttgggcgtta attgtcgaca tgatcgcagc cataggtgca
     4981 gccagaaaga tcccggcagc aaacggtggc agcactttta ccattaacgt cgggatcacc
     5041 agatccggta cggtgagatc ggggatcact gcacgaccta acgctccggc taggtgcata
     5101 ccgaacatca ggattgcgac tacaatcgta ccgatgatga tcccccggtg taccgctttg
     5161 ctgtctttat aagagataca gcgcaccgca gtatgcggca ggccaatcac gccaaagcac
     5221 accagtaccc agaacgacgt cataaaggca ggcgacagaa tatcgtcagc gccttgtggc
     5281 gtaaccagtt gcggatcgat ggtttgcaag gtctgtactg cgttgcttaa gccgccagcg
     5341 gcatgtacca cgccaataag cagcacaacg gtgccaatca gcatcacaag cccttgcatg
     5401 gtgtcgttca gcacgctggc gcgaaagccg ccaaaggcgg tatacaacgc aatgctgata
     5461 ccgaaaatca gcagcccggt ttcataagga atacccgccg cggtttccag caggcgcgca
     5521 ccgccgataa actgcacggt cattgcccca acgaacgcaa ccagcaaact caaactcgcc
     5581 agccacacca gaagacgact ctggtagcgg gcaaacagca tgtcgttcag cgtcactgca
     5641 ttgtagcggc gcgcaagaat cgcaaacttc ttgccgagaa taccgagtga aagccagact
     5701 gcaggaagct gaatcatcgc cagcaatacc cagcccagcc cgtatttata agcagctcct
     5761 ggcccgccga taaacgaact ggcactgata taggtcgcgg tgagcgtcat cgccagcaca
     5821 ataccgccca tagagcggct gccgaggaaa tactcattaa ggaaggtgcc ggtgctccgt
     5881 ttacgcatcg cataaaccga gataccgaac accaccacca gataggcgac cagcggtaga
     5941 attacttcaa gctgcatcgt catcctccag tgggatatcg cgatagataa atttcaccat
     6001 cgcccagcac agtccaataa acagtagcgg cgtcaggatg caggccatct caaaccagcg
     6061 cggaaagccg gtaaaaccgg gggcaacgcc aggtaagtaa gcggctacta accaaactgc
     6121 cagatacaaa agggtcagcc ccagcgccca gcgcgcctct ttatgggcct gaacaaaacg
     6181 agtgtccatt ttttgtccct gatgggtgaa gaaagcgagg attgtacctt atgggggttg
     6241 tcgatcccca gtaataaaaa aggccggaaa atccggcctt ttggcgcttt tgcagtctta
     6301 tttttcctga agaccgagtt ttttctccag atagtggatg ttagtgccac catgctggaa
     6361 gttctcgtca ttcatgatgc ggatctgcag atcaacgttg gttttgatac cgtcgatgat
     6421 cagctcctgc agcgcattct tcatgcgggc aatcgccacg tcacggtttt caccgtagca
     6481 aatcagctta ccgatcattg agtcatagta cggcggtacg gtgtagcccg cgtagatatg
     6541 agactcccaa cgtacgccaa aaccgccagg tgcgtggaaa cgggtgattt tgcctgggct
     6601 tggcaggaag gtgttcggat cttcggcgtt gatacgacat tccaccgcat ggccgcgaac
     6661 gtgaacttct tcttgcttga tcgacagcgg ttgaccggca gcgatacgga gctgttcttt
     6721 gatcaggtca acgccagtga tcatttcggt aaccgggtgt tctacctgaa tacgggtgtt
     6781 catttcgatg aaatagaact cgccgttttc gaacagaaac tcgaaagtac ctgcaccgcg
     6841 atagccgata tcaacacacg ctttagcgca acgttcgccg atgtagcgac gcagttccgg
     6901 ggtaatgccc ggtgctggcg cttcttcgac cactttctgg tggcggcgct gcatggagca
     6961 gtcacgttcc gccagataga tagcgttgcc ccgaccgtca gccagtacct gaatctcgac
     7021 gtggcgagga ttttccaggt atttctccat gtaaaccata tcgttgctga aagcagcttt
     7081 cgcttccgca cgggtcatgg agatggattg tgccagttca gcgtcgccgc gcactacgcg
     7141 cataccgcga ccaccgccgc cgccggaggc tttgataatc accggataac caatgcgttt
     7201 agcaatggca cggtttttat ccatatcgtc gcccaacggg ccgtcagaac ccggtacgca
     7261 agggacgccc gcttttttca tcgccgcgat tgcggatact ttgtcgccca tcaggcgaat
     7321 ggtttctgct ttcgggccga tgaagataaa gccggagcgt tcaacctgct cggcaaagtt
     7381 ggcgttctcg gagaggaagc cgtaacccgg atggattgct actgcgccgg tgatttcagc
     7441 ggcgctgatg attgccggga tgttcagata actttttact gacggagcag ggccaataca
     7501 gaccgtttca tctgccagta atacgtgttt tagatcgcga tccgcgctgg agtgcacagc
     7561 gacagtcttg atgcccagtt ctttacaggc acgaagaata cgcaatgcaa tctcgccgcg
     7621 gttggcaata acaattttat ccagcatgtt cgcctcgtta ctcgatgacg accagcggct
     7681 cgtcaaattc taccggttgt ccactttcga ccagaattgc tttcacggta ccggatttgt
     7741 ccgcttcgat ctggttcatc attttcatgg cttcaacgat gcacagggta tcgcccacgt
     7801 tgactttctg acccacttcg atgaacgctt ttgcgtccgg gcttggggtg cggtagaaag
     7861 taccaaccat cggggaacgt acgatgtgac cactgatttc cgctgctgct ggcgcttcca
     7921 tggaaggaac ggtcgccgga gcggctgcgt tagattgagc tggctgctgc atcattggtg
     7981 cagcgtaagc ttgttgcatc acagggaaac ttgcggcagg agctgcacgg ctaatgcgta
     8041 ctgactcttc gccttcagaa atttccagtt cggagatgcc tgattcttca accagctcga
     8101 tcagtttttt aatcttacga atatccatga gtgggttccg tactctttgt ttagtgtgat
     8161 tgtgacaggc gtttcaccgc cgtctgtaaa gcgtatgccc cgacagctgt atgcatagcg
     8221 ataaattcca gcaggccggg acgccggtct attttgcccc agagtatcaa tgttagactt
     8281 gacggacatt gtgcagcgat ctgctgctat cctccggcaa aaaacaaaat ataccttcgt
     8341 tgcgccaatg tcaccttttc cgcagcgcaa aaactgcgtc agggaggacg aggtcgcaca
     8401 ttataacgat ttcgtagcaa ttggcagcta aatactggtc tcatcaggga agataatcaa
     8461 cagctaacat gtaaataacc ttcaacaccg tgtaatttgc aacaagccgc acaattcacg
     8521 aaattagcgc catcatcgac ggaacttctt ataacgtaag gtgaaaagct gtaaagccag
     8581 ccctgcgaag atgaggggct gcaatgagat aatcttcaca gaccacaaat aatgtaggga
     8641 tgccaggatc gcgacaagat agacgacatt acgcagcggt tgctggggat tacccaattt
     8701 tcactgcatc gtctggatag atgtgaatgc taaagcacgc aaaatcaacc gactgacaat
     8761 tctcagcatt aaataggttc gagtggttaa tttgtagtca aataaaatca gattattcac
     8821 cccatggtac cagaaatcag taacaggtta agcgtagtgt cgcccaggca aagcaccata
     8881 aacctaacag gcgacggagg cgtatcaata atggctgttt agaggagtat caatgcgcat
     8941 caatttgtgg tcagtcgaaa tcatgacttt ccagtttgga tgatgttgta caacttcctg
     9001 accaaggtgt tcgacgaaag ctgtccggct aaccgctttt cccgttaacc gattgaataa
     9061 tgatgtcaaa atgagttccc tctcttatta ttccttgcta aatggttagt taaccttcac
     9121 cagcgtgcga ccctggatct ggttattaat gatggcctcg gcaaagttcg gtgcctctga
     9181 cagagatatc tcttttgccg cctgggtata gaatgattcc ggtaaatcgg cgaccagtcg
     9241 ctgccaggct tgtgcgcggc gttctggtgg cgtcattact gaatccaccc cttgcaaacg
     9301 gacattacgc agaataaatg gcatgaccgt ggttggcaga gtaaaaccac ccgccagacc
     9361 acaggccgcc acgcagccgc cgtaattcat ttgcgccagc acttttgcca gcactttgtc
     9421 gccaacagtg tcaattgccc cagcccagac ttgtttttcc agaggacggg attcggcaaa
     9481 ctcatcacga gggagaatac ggctggcacc taaacttttc agatattcat gggtactttc
     9541 gcgaccggaa acggcaacga cctgataacc caacttatgc agcagcgcca cggcggtact
     9601 gccgacgcca ccactggcac ccgtcacgac aatctccccg tcctgcgggc gaacaccggc
     9661 atcttccagc gccatcacac acagcatggc ggtaaaaccg gcagtaccga taatcattgc
     9721 tttacgcgcg tccagccctt gcggcatggc aaccagccag tcacctttca ctcgcgcctg
     9781 ctccgccagc ccaccccagt ggttttcacc aacgccccag ccagtgagta acacctcctg
     9841 accggcatga aaacgcggat cttcgctggt gcgtacagtt ccggcaaaat cgatcccagg
     9901 aatcatcgga aaattacgga tgatttttcc cttaccggta atcgccagcg catctttata
     9961 gttcaggctc gaccagtgaa catcgaccgt gacatcgccc tccggcaggc gactttcgtc
    10021 cagagtctgt actgatgcga gagttttgcc gtcctgctgt tctaaaagta acgcctgcat
    10081 aagtggtcct catgtgcatg atggattgga aaataatttc tgaagattat actcgctaat
    10141 ggaaaagcaa agccgatgaa gcgcaataaa ttgcgagata aatctgattt gctagtatgc
    10201 ccgcttcctc actatcggag ttaacacaag gatgagatta acgacgaaat tttcggcctt
    10261 tgttacgctg ctcaccgggt taacaatttt tgtgactttg ctgggctgtt cgctaagttt
    10321 ctacaacgca attcagtata agtttagtca tcgcgttcag gcggtggcga cggcgattga
    10381 tacccacctt gtgtcgaatg acttcagcac attaaggcca caaattaccg aattaatgat
    10441 gtcggcagat atcgttcgtg tagacctgct ccatggtgat aaacaggttt ataccctggc
    10501 cagaaatggt agttatcgtc cggttggctc cagcgatctg tttcgcgaac tgagtgttcc
    10561 gttgataaag catccgggga tgtcgctgcg tctggtttat caggatccga tgggcaacta
    10621 tttccattct ttaatgacta ccgcgccgct cacgggggcg attggcttta tcattgttat
    10681 gctcttcctg gcggtacgct ggttacaacg gcaacttgcc gggcaagaat tgctggaaac
    10741 ccgggctact cgtatcttaa acggtgagcg tggctctaat gtgttgggaa ccatctatga
    10801 atggccgccc agaaccagca gtgcgctaga tacgctgctt cgtgaaattc agaacgcacg
    10861 cgaacaacac agccgtcttg atacgctgat ccgctcttat gccgcccagg acgtgaaaac
    10921 cggcctcaat aaccgactct ttttcgataa tcagttagca acgttactgg aagatcagga
    10981 gaaagtaggt acccacggga tcgtgatgat gattcgtctg ccggatttca atatgttgag
    11041 cgatacttgg gggcacagcc aggttgaaga acagttcttc actctgacta atctgctgtc
    11101 gacatttatg atgcgctacc ctggcgcact gctggcgcgt taccaccgca gtgattttgc
    11161 tgcgctgtta ccgcaccgga cgttaaaaga ggcagagagc atcgccggtc agttaatcaa
    11221 agccgtcgat accttgccga acaataaaat gctcgatcgc gacgatatga tccacattgg
    11281 tatctgcgcc tggcgtagtg gtcaggatac cgagcaggta atggaacatg cagagtctgc
    11341 cacgcgtaat gcgggattgc agggcggcaa tagctgggct atttacgatg actcgttgcc
    11401 tgaaaaagga cgcggtaatg ttcgctggcg tacgcttatc gagcaaatgc tcagtcgcgg
    11461 cggcccgcgc ctttatcaaa aaccggcggt tactcgcgaa ggtcaggttc atcatcgcga
    11521 actcatgtgc cgcatcttcg atggtaatga agaggttagc tcggcggagt atatgccgat
    11581 ggtcttgcag tttggcttat cggaagagta tgaccgtctg caaatcagcc gtcttattcc
    11641 actattgcgt tactggccag aggaaaatct ggcgattcag gttaccgttg agtcgctgat
    11701 tcgcccgcgt tttcagcgtt ggctgcgcga tacgttaatg caatgtgaaa aatcacaacg
    11761 aaaacgcata attattgaac ttgcagaggc cgatgtaggt caacatatca gtcgtttaca
    11821 acctgttatt cgtttagtga atgctttagg ggtacgggta gccgtcaacc aggctggttt
    11881 gacgctggta agcaccagtt ggatcaaaga acttaatgtt gagttactca agctccatcc
    11941 ggggctggtc agaaacattg agaagcgaac ggagaaccag ctgctggttc aaagcctggt
    12001 ggaagcctgc tccgggacca gcacccacgt ttacgccacc ggcgtgcgtt cgcgaagcga
    12061 gtggcagacc ctgattcagc gcggtgttac aggcgggcaa ggggattttt tcgcgtcctc
    12121 acagccactt gatactaacg tgaaaaaata ttcacaaaga tactcggttt aacctgccgt
    12181 ttaatccgtt ttcacgtaga ataacgcgcg ctgcgtctca tgggagtgtg cttgtctgct
    12241 cgccagattg ttgcagcaca tatgcagatg aatgacctta cgcggttgca aacaggcgag
    12301 gaatgctgct gatgcattaa gcctttctgg actcaggcag agatttgtaa caaaggaaac
    12361 gaactgcact aattttcacc gtagcagatg atttttgcgc cttgtcgctg ctgcgtgtgg
    12421 ttggtaaagt aagcggattt tcttttccgc cccagctttc aggattatcc cttagtatgt
    12481 tgaaaaaatt tcgtggcatg ttttccaatg acttgtccat tgacctgggt actgcgaata
    12541 ccctcattta tgtaaaagga caaggcatcg tattgaatga gccttccgtg gtggccattc
    12601 gtcaggatcg tgccggttca ccgaaaagcg tagctgcagt aggtcatgac gcgaagcaga
    12661 tgctgggccg tacgccgggc aatattgctg ccattcgccc aatgaaagac ggcgttatcg
    12721 ccgacttctt cgtgactgaa aaaatgctcc agcacttcat caaacaagtg cacagcaaca
    12781 gctttatgcg tccaagcccg cgcgttctgg tttgtgtacc ggttggcgcg acccaggttg
    12841 aacgccgcgc aattcgtgaa tccgcgcagg gcgctggtgc ccgtgaagtc ttcctgattg
    12901 aagaaccgat ggctgccgca attggtgctg gcctgccggt ttctgaagcg accggttcta
    12961 tggtggttga tatcggtggt ggtaccactg aagttgctgt tatctccttg aacggcgtgg
    13021 tttactcctc ttccgtgcgc attggtggtg atcgtttcga cgaagctatc atcaactatg
    13081 tgcgtcgtaa ttacggttct ctgatcggtg aagccaccgc agaacgtatc aagcacgaaa
    13141 tcggttcggc ttacccgggc gatgaagtcc gtgaaatcga agttcgtggc cgtaacctgg
    13201 cagaaggtgt tccacgcggt tttaccctga actccaacga aatcctcgaa gcactgcagg
    13261 aaccgctgac cggtattgtg agcgcggtaa tggttgcact ggaacagtgc ccgccggaac
    13321 tggcttccga catctccgag cgcggcatgg tgctcaccgg tggtggcgca ctgctgcgta
    13381 accttgaccg tttgttaatg gaagaaaccg gcattccagt agttgttgct gaagacccgc
    13441 tgacctgtgt ggcgcgcggt ggcggcaaag cgctggaaat gatcgacatg cacggcggcg
    13501 acctgttcag cgaagagtaa tcggatgcag gcaggggaaa tgtctgttta ccctgcctgg
    13561 tctgatacga gaatacgcat aacttatgaa gccaattttt agccgtggcc cgtcgctaca
    13621 gattcgcctt attctggcgg tgctggtggc gctcggcatt attattgccg acagccgcct
    13681 ggggacgttc agtcaaatcc gtacttatat ggataccgcc gtcagtcctt tctactttgt
    13741 ttccaatgct cctcgtgaat tgctggatgg cgtatcgcag acgctggcct cgcgtgacca
    13801 actagaactt gaaaaccggg cgttacgtca ggaactgttg ctgaaaaaca gtgaactgct
    13861 gatgcttgga caatacaaac aggagaacgc gcgtctgcgc gagctgctgg gttccccgct
    13921 gcgtcaggat gagcagaaaa tggtgactca ggttatctcc acggttaacg atccttatag
    13981 cgatcaagtt gttatcgata aaggtagcgt taatggcgtt tatgaaggcc agccggtcat
    14041 cagcgacaaa ggtgttgttg gtcaggtggt ggccgtcgct aaactgacca gtcgcgtgct
    14101 gctgatttgt gatgcgaccc acgcgctgcc aatccaggtg ctgcgcaacg atatccgcgt
    14161 aattgcagcc ggtaacggtt gtacggatga tttgcagctt gagcatctgc cagcgaatac
    14221 ggatattcgt gttggtgatg tgctggtgac ctccggtctg ggcggtcgtt tcccggaagg
    14281 ctatccggtc gcggttgtct cttccgtaaa actcgatacc cagcgcgctt atactgtgat
    14341 tcaggcgcgt ccgactgcag gactgcaacg tttgcgttat ctgctgctgc tgtggggcgc
    14401 ggatcgtaac ggcgctaacc cgatgacgcc ggaagaggtg catcgtgttg ctaatgaacg
    14461 tctgatgcag atgatgccgc aggtattgcc ttcgccagac gcgatggggc caaagttacc
    14521 tgaaccggca acggggatcg ctcagccgac tccgcagcaa ccgacgacag gaaatgcagc
    14581 tactgcgcct gctgcgccca cacagcctgc tgctaatcgc tctccacaaa gggctacgcc
    14641 gccgcaaagt ggtgctcaac cgcctgcgcg tgcgccggga gggcaatagt ggcgagctat
    14701 cgtagccagg gacgttgggt aatctggctc tctttcctca ttgcgctgtt gctgcaaatc
    14761 atgccctggc cggataacct gattgttttc cggccaaact gggtgttact catcttgttg
    14821 tattggatcc tggccttgcc tcatcgcgta aatgtgggca caggttttgt gatgggtgcc
    14881 atactggatc tgatcagcgg ctcgacgctt ggcgtacgcg tattggcgat gagcatcatt
    14941 gcttacctgg tggcgctgaa ataccagctt ttccgcaacc tcgcattatg gcagcaggcg
    15001 ctggtcgtca tgttgctttc gctggtggtg gatattattg ttttctgggc agagttttta
    15061 gtgattaacg tctctttcag accggaagtg ttctggagta gtgtagtcaa tggggtgctc
    15121 tggccgtgga ttttcttgct gatgcgtaaa gtccgtcagc agtttgcagt gcaataaagg
    15181 tttctatgac ttctctgtat ttagcttccg gttctccgcg tcgtcaggag ttacttgcgc
    15241 aacttggtgt gacctttgaa cgtattgtta cgggcattga ggagcagcgt cagccgcagg
    15301 agagcgcgca gcagtatgtt gtgcgtctgg cgcgcgagaa agcacaggcg ggtgtcgcgc
    15361 aaacggcgca ggatctcccg gtgctgggtg cggatactat cgttatcctg aatggagaag
    15421 tgctggagaa accgcgcgac gcagagcatg cggcgcagat gttgcgcaaa ttatcgggtc
    15481 agacccatca ggtgatgacg gcagtggcgt tggccgacag ccagcacatt ctcgattgcc
    15541 tggtggtcac cgatgtgact ttcagaacgt taacagacga agacatcgcg ggctatgtcg
    15601 ccagcggtga accgttagat aaagcaggtg catacggtat tcaggggctg ggtggctgtt
    15661 ttgtcaggaa gataaatggc agctatcacg ccgtagtcgg cttacctctg gttgaaacgt
    15721 atgaattatt aagtaatttt aacgcactgc gtgagaaaag ggataaacat gacggctgaa
    15781 ttgttagtaa acgtaacgcc ttcggaaacg cgagtggcgt atattgatgg cggtattctg
    15841 caggaaattc atattgaacg tgaggcgcga cgcggaatag taggcaatat ctacaagggt
    15901 cgtgtaagtc gtgtacttcc gggtatgcag gcggcttttg tagatattgg gctggataaa
    15961 gccgcgtttc ttcatgcatc cgatatcatg ccgcacaccg aatgtgtggc gggtgaagaa
    16021 caaaagcaat tcacggtgcg cgacatctcg gaactggttc gtcaggggca agatctgatg
    16081 gtgcaggtgg tgaaagatcc gcttggcact aaaggtgcgc gcctgaccac cgatatcacg
    16141 ctgccttctc gctatctggt ctttatgcca ggggcttctc acgttggggt ttcccaacgt
    16201 attgaaagcg aatcagaacg tgaacgcctg aaaaaagtgg tcgcagagta ttgcgacgag
    16261 cagggcgggt ttatcatccg taccgcagcg gaaggggttg gcgaggctga actggcctcc
    16321 gatgccgctt atctgaaacg cgtctggacc aaagttatgg agcgtaaaaa gcgcccgcag
    16381 acccgttatc agctgtacgg cgaactggcg ctggctcagc gtgttctgcg tgatttcgcc
    16441 gacgccgaac tggaccgcat tcgcgttgac tcacgcctga cttacgaagc attacttgag
    16501 ttcacctcgg agtacattcc cgagatgacc agcaagctgg agcattacac cggacgccag
    16561 ccgattttcg atctctttga tgtcgaaaac gaaatccagc gagcgctgga acgcaaagta
    16621 gaactgaaat ccggtggtta tctcattatc gaccagaccg aagcgatgac caccgtggac
    16681 atcaataccg gagcgtttgt cggtcatcgc aatctggacg acaccatttt caataccaat
    16741 attgaagcga cgcaggctat cgctcgccag ttacggttgc gtaatctggg cgggattatc
    16801 attattgatt tcatcgatat gaataatgaa gatcaccgcc gccgcgtgct gcactcgctg
    16861 gagcaggcgt tgagcaaaga ccgggtgaaa accagcgtta atggtttttc ggcgctgggg
    16921 ctggtggaga tgacgcgtaa acgcacccgc gaaagcattg agcacgtact gtgtaacgaa
    16981 tgcccaacct gccacggtcg cggaacggtg aaaaccgtgg aaacggtatg ctatgaaatc
    17041 atgcgcgaga ttgttcgtgt ccaccatgct tacgactccg accgtttcct ggtctatgct
    17101 tctccggcag tagccgaagc cttgaaaggc gaagagtcac actcgctggc ggaagtggaa
    17161 attttcgttg gcaaacaggt taaagtacaa attgaaccgc tctataacca ggagcagttt
    17221 gacgtcgtaa tgatgtaaac agatgctggg ccgccatccg gcaaagggtt tttgagtcac
    17281 atttttagca gacaaggagt gacgggtgag gcgattgccg gggattttac tgcttactgg
    17341 agccgcgctc gttgtgatcg ctgccctgct ggttagcggc ctgcgtattg ctttaccgca
    17401 tcttgacgcc tggcgtccgg aaatcctcaa caaaatagaa tccgcgactg gcatgccggt
    17461 agaagccagt cagctctcag ccagctggca gaattttggc ccgacgcttg aagcacacga
    17521 catccgtgca gaactaaaag atggcggcga attttcggtt aaacgcgtta ctctggcgct
    17581 ggatgtctgg cagagcctgt tacatatgcg ctggcagttt cgcgacctca ctttctggca
    17641 gctgcgcttt cgcaccaaca ctcctatcac cagcggtggt agcgatgaca gtctggaagc
    17701 cagtcacatc agcgatctgt ttcttcgtca atttgaccat ttcgatctcc gcgacagtga
    17761 agtcagtttc ctgacgccat ccggtcagcg tgccgagctg gcgatcccac aactcacctg
    17821 gctgaacgat ccacgtcgac accgtgcgga aggcctggta agcctctcca gccttaccgg
    17881 acagcacggc gtgatgcagg tgcgcatgga tttgcgcgat gatgaggggt tgttaagcaa
    17941 tggtcgcgtc tggctacagg cggatgacat cgacctgaag ccgtggctcg gtaaatggat
    18001 gcaggacaat attgctctgg aaacggcaca gttctccctt gaaggctgga tgacgatcga
    18061 caaaggcgat gtaaccggcg gtgacgtctg gctgaaacag ggcggtgcca gctggttggg
    18121 cgagaagcaa acgcatacgc tgtcggtgga taatctgacc gcgcatatta cgcgtgaaaa
    18181 tccgggctgg cagttctcta ttcccgatac acggatcacg atggacggca aaccctggcc
    18241 gagcggagca ttgacgctgg cctggatacc ggaacaggac gttggcggca aagacaataa
    18301 acgcagtgac gaactccgga ttcgcgccag taatctggag ctggcaggcc tggagggcat
    18361 acgcccgctg gccgcgaaac tttcacctgc actgggtgat gtttggcgct ccacacaacc
    18421 gagcggcaag attaacactc tggcgctgga tatcccgctt caggcggcag acaagacccg
    18481 ttttcaggca tcgtggagcg atctggcctg gaagcaatgg aaattattac cgggtgcgga
    18541 acacttctcc gggacgcttt ccggcagcgt tgaaaatggt ttgcttaccg cgtcgatgaa
    18601 gcaggcaaag atgccttacg aaacggtatt ccgtgcgcca ctggaaatcg ccgacggcca
    18661 ggcaactata agctggctga acaatgacaa aggtttccag ctggatgggc gtaatattga
    18721 cgttaaagcc aaagccgtcc atgcgcgcgg cggttttcgt tacctgcaac ctgctaacga
    18781 tgaaccctgg ctgggtattc tggctggcat cagtaccgat gatggttcac aagcctggca
    18841 ctatttcccg gaaaacttga tgggtaaaga cctggttgat tacttaagtg gcgcgattca
    18901 gggcggtgaa gcggataacg cgacgctggt ttatggtggc aatccgcaac tcttccccta
    18961 taaacacaac gaaggtcagt ttgaagtgct ggtgccgctg cgcaacgcga agtttgcctt
    19021 ccagccggac tggcctgcat taactaacct tgatattgaa ctggacttta ttaacgacgg
    19081 tttatggatg aaaaccgatg gcgttaatct gggcggcgtg cgcgcgagta atctcaccgc
    19141 agtgatccct gactactcga aagaaaaact gctgattgac gctgacatta aaggtccggg
    19201 taaagccgtt ggcccttact ttgatgagac accgctgaaa gattctctgg gtgcgaccct
    19261 gcaagaactc cagctcgacg gcgatgtgaa tgctcgctta catcttgata tcccgctgaa
    19321 cggcgagctg gtaaccgcga aaggtgaagt gacgctgcgt aataacagtc tgtttatcaa
    19381 accactcgac agcaccctga aaaatttgag cggtaaattc agctttatca atggcgatct
    19441 gcaaagtgaa ccactgacag caagctggtt taatcagccg ttgaacgtgg atttttccac
    19501 caaagaaggg gcaaaagcct accaggttgc ggtgaatctc aacggtaact ggcaaccggc
    19561 gaaaaccggc gttctgcctg aagcggtgaa cgaagcattg agtggcagcg tggcgtggga
    19621 tggtaaagtg ggcattgatc tgccttatca tgctggcgcg acctataacg tagagctgaa
    19681 cggcgatctg aataatgtga gtagtcactt accttcaccg ttagccaaac ctgcgggtga
    19741 accactagcg gtaaacgtta aggttgatgg caatctcaac agctttgaat taaccggaca
    19801 ggctggtgcg gataaccatt tcaatagccg ctggttgctt ggtcaaaagc tgacgctcga
    19861 tcgtgctatt tgggcggcag acagtaaaac gctcccgccg ttgccggaac aaagtggcgt
    19921 tgaactcaat atgccgccga tgaatggtgc cgagtggctg gccctgttcc agaaaggcgc
    19981 tgcggagagt gtcggtggtg cagcgagttt cccacaatac ataacgttac gtacgcctat
    20041 gttgtcactg ggaaatcagc aatggaataa cctgagtatt gtttcgcaac cgacggcaaa
    20101 tggcaccctg gttgaggcgc aagggcgtga aatcaacgcc acgctagcga tgcgtaataa
    20161 cgcgccgtgg ctggcgaata tcaaatatct ttattacaac ccgagcgtgg cgaaaactcg
    20221 tggtgattca acgccgtcat cacctttccc gacaacggag cgcattaact tccgtggctg
    20281 gccggacgca caaatacgat gcacagagtg ctggttctgg gggcaaaaat tcggtcgcat
    20341 tgacagtgat atcaccattt ctggcgatac gttaacgctg accaatggac tgattgatac
    20401 tggtttctcg cggcttactg ccgatggtga atgggttaat aatccgggga atgaacgtac
    20461 ctcgctgaaa ggaaaactgc gcgggcagaa aattgatgcc gccgcagaat tttttggtgt
    20521 cacgacgccc atacgccagt cgtcatttaa tgtggattac gatttacact ggcgcaaagc
    20581 accctggcag ccagatgaag cgacgttgaa tggcatcatt catactcaac tgggtaaagg
    20641 cgaaattacc gaaatcaata ccggacatgc cgggcaattg ctgcgcttat tgagcgtaga
    20701 tgccctgatg cgtaagctgc gttttgattt cagagacact tttggcgaag ggttctattt
    20761 tgactccatt cgcagcaccg cgtggattaa agacggggtt atgcacaccg acgacacgct
    20821 ggtggatggc ctggaggcgg atatcgccat gaaagggtcg gtaaatctgg tacgtcgcga
    20881 cctgaatatg gaagcggttg tcgcaccaga gatttctgcg acggtgggcg tggctgcggc
    20941 ttttgcggtt aaccccattg ttggcgcggc agtttttgcc gctagtaaag tgctggggcc
    21001 gctgtggagc aaagtctcca ttttgcgcta tcacatttcg ggtccgctgg acgatccgca
    21061 aatcaacgaa gtgttgcgcc aaccgcgtaa agaaaaagcg caatgatttg acgagggcgc
    21121 gtaattgccc caatctcata tgataatcgt tgccaaaggc caacgagcca gaacataacc
    21181 gtaggccgga tagggcgttc atgccgcatc cggcagccgt aaaaaatcct ctactgcagt
    21241 aactaacgag tagcaaaaac gatgagtctg aacctggtaa gtgaacaatt gctagcggcg
    21301 aacagcctga aacatcagga cttgttcgcg atcctcggtc aactggccga acgtcgcctt
    21361 gattatggcg atctctattt tcagtcgagc tatcacgaat cctgggtttt agaagaccgc
    21421 attattaaag atggttctta caacatcgat cagggcgttg gcgtgcgtgc aatcagcggt
    21481 gaaaaaaccg gatttgctta cgctgaccaa atcagcctgc tggcgctgga acagagtgcg
    21541 caagcggcgc gcaccatcgt ccgtgatagt ggtgatggca aagtacagac gctgggcgcg
    21601 gtagagcata gcccgttgta tacctcggta gatccgctgc aaagcatgag ccgtgaagag
    21661 aagctggata tcctgcgtcg cgtcgataag gttgctcgcg aagcggacaa gcgcgtacag
    21721 gaagtgactg ccagcctcag tggtgtctat gaattaattc tggttgcggc caccgacggc
    21781 acgctagcgg cggatgtccg tccgctggtg cgtctttccg tgagcgttct ggtcgaagaa
    21841 gatggcaaac gcgaacgcgg tgccagtggc ggcggcggtc gttttggtta tgagttcttc
    21901 cttgccgatc ttgacggcga agtccgtgcg gatgcatggg caaaagaagc agtgcgtatg
    21961 gcgctggtca atctttctgc cgttgctgca ccagcgggca ccatgccggt agtacttggc
    22021 gcaggttggc cgggcgtgct gttgcatgaa gcggtaggtc acggtctgga aggcgacttc
    22081 aaccgccgcg gcacttcagt atttagtggg caggtcgggg agctggtggc ttcagaactg
    22141 tgtaccgtgg ttgatgacgg cacgatggtc gatcgccgtg gttcggtggc gattgatgac
    22201 gaaggtacgc caggccagta caacgtgctg attgagaacg gcattctgaa aggctacatg
    22261 caggataaac tcaacgcgcg tttgatgggg atgacgccga ctggcaacgg tcgccgtgaa
    22321 tcctacgccc atctgcccat gccgcgtatg actaacacct atatgctgcc gggtaaatcg
    22381 accccgcagg aaattattga atccgttgag tacggtatct atgcaccgaa ctttggtggc
    22441 ggtcaggtgg atatcacctc cggcaaattc gttttctcca cttcagaagc atatctgatt
    22501 gaaaacggta aagtaacgaa gccggtgaaa ggcgcaacgt tgattggttc cggtatcgaa
    22561 accatgcagc agatttcgat ggttggcaac gacctgaaac tggataacgg cgtgggtgtc
    22621 tgcggtaaag aagggcaaag tttgccggtt ggcgtgggcc agccaacgct gaaagttgat
    22681 aacctgactg ttggcggtac tgcgtaataa tttcattatt ttcagaagga taattaatct
    22741 ttctacgtgc acgaacggtc ccctcgctcc tctggggtta gggtgagggg aacccgttgg
    22801 cacaggtttg tagaacgtaa cagtacaata tgaattactt ctctttcccg cgcccgtgca
    22861 tctcctgaaa caatttaccg acctcaacaa aataatccgt cagcgagttg atcacgacct
    22921 gtaccttcag cggcagctta tctttttcgg tatataacgc ataaaccggg cgtggatctg
    22981 actgataacg cggcagcagg atctccagct caccgcgatt gatctcgttg atcacccaca
    23041 tcagcggcac gtaggcgatc ccggcacccg ccgtcagcca gcgcaccagc gtcatcggat
    23101 cattagtcac aaatcttcct tgtgggatca ggcgagtcga gatcccttcc ggtgcgatca
    23161 gttcaaattc attgtcgggc cgcacgctgt attcaagcca tgaatgacta ctcaaatcgg
    23221 cgggtttttc cggtatgccg tattgtgtga gatagctttt cgcggcgcac accaccatcg
    23281 gcatcgcgcc cagacggcgg gaaaacaggc tggaattccg gtaaccagat tgacgctcaa
    23341 acctgggtat tctttcagca tttttgctgt cagcccggcg agaacatttt gtgccatagt
    23401 tgaagaacag ccaatgcgca gcgtcccgat gggggtgtta ttgaaggcat acagttgctc
    23461 atgaacatcc tgcacttcat gaagcatccg acggcagccc tggtagtaaa ttctgccggc
    23521 ttcggtcagg ccaatgctgc gtgtgctacg gtttaacagc tttacctgca actcatcttc
    23581 cagttttgac accgtctgac tgatggacga aacgctcatc tgtagctgtc tggcggcggc
    23641 ggtaaaagag ccaaattcaa ctactttggc aaacaccgac atgcgtttta gtcgttccat
    23701 tattcactct gacttaaaag tgatttagat cacataatat agataacagc ataacagtta
    23761 cgctaatata ttaaatatca atctacagca atgttgctct cgcccggctt tccatgccct
    23821 tctctgcggc gacagatgct gaaaataata acgcctgctc tctctttaca accaaggtca
    23881 acatgagtct gtttcccgtt atcgtggtgt ttggactgtc cttcccaccg atattttttg
    23941 aattgctttt atcactggcg attttctggc tggtgcgccg ggtacttgtg ccaacaggta
    24001 tctacgactt tgtctggcat ccggcgttgt tcaacaccgc gctctattgc tgcttgtttt
    24061 atttgatatc gcgactgttc gtttgaggtt gaagtgaaaa cactaataag aaaattctcc
    24121 cgtacggcca tcacggtcgt attagtcatt ctagccttca tcgcaatttt taatgcctgg
    24181 gtctattaca ccgaatcccc ctggacgcgt gacgcgcgct ttagcgctga cgtcgttgcg
    24241 atcgcgccgg acgtttctgg actcattacc caggtgaatg ttcatgataa ccagctggtg
    24301 aaaaaaggac agatactgtt caccatcgac cagccgcgct atcaaaaggc gcttgaggaa
    24361 gcgcaagccg atgttgctta ttatcaggta ctggcacagg agaaacgcca ggaggccgga
    24421 cgtcgtaacc gtctcggtgt gcaggcgatg tctcgcgaag agatcgacca ggccaacaac
    24481 gtactacaaa cggttctgca tcagttagcg aaagcgcagg cgacccgcga tctggcaaaa
    24541 ctggatcttg aacgcacggt gatccgcgcg ccagcagatg gctgggtgac caacctcaac
    24601 gtctataccg gtgagtttat tactcgagga tcaacggcgg ttgcgctggt gaaacagaac
    24661 tccttctatg tactggccta tatggaagaa actaagctgg aaggggggcg tccggggtat
    24721 cgtgcagaga tcacgccgct tggcagtaac aaagtgctga aagggactgt tgatagtgtt
    24781 gccgcagggg tcaccaacgc cagcagcacg cgtgacgaca aagggatggc gactatagac
    24841 tctaatcttg aatgggtgcg tcttgcgcaa cgtgttccgg ttcgtattcg tctcgacaac
    24901 cagcaagaga acatctggcc tgcgggcacc actgctacag tggtggtcac tggcaaacaa
    24961 gatcgcgacg aaagccagga ttcgttcttc cgtaaaatgg cccatcgcct gcgtgagttt
    25021 ggttaatcac gatgggtatt ttctccattg ctaaccaaca tattcgcttt gcggtaaaac
    25081 tggcgaccgc cattgtactg gcgctgtttg ttggctttca cttccagctg gaaacgccac
    25141 gctgggcggt actgacagcg gcgattgttg ccgccggtcc ggcctttgct gcgggaggtg
    25201 aaccgtattc tggcgctatt cgctatcgtg gctttttgcg catcatcggc acatttattg
    25261 gctgtattgc cggactggtg atcatcattg cgatgatccg cgcaccatta ttgatgattc
    25321 tggtgtgctg tatctgggcc ggtttttgta cctggatatc ctcgctggta cgaatagaaa
    25381 actcgtacgc gtgggggctg gccggttata ccgcgctgat cattgtgatc accattcagc
    25441 cggaaccatt gcttacgccg cagtttgccg tcgaacgttg tagcgagatc gttatcggta
    25501 ttgtgtgtgc gattatggcg gatttgctct tttctccgcg atcgatcaaa caagaagtag
    25561 atcgagagct ggaaagtttg ctggtcgcgc aatatcaatt aatgcaactc tgtatcaagc
    25621 atggcgatgg tgaagttgtc gataaagcct ggggcgacct ggtgcgacgc accacggcac
    25681 tacaaggcat gcgcagcaac ctgaatatgg aatcttcccg ctgggcgcgg gccaatcgac
    25741 gtttaaaagc gatcaatacg ctatcgctga cgctgattac ccaatcctgc gaaacttatc
    25801 ttattcagaa tacgcgcccg gaattgatca ctgatacttt ccgcgaattt tttgacacgc
    25861 cggtagaaac cgcgcaggac gtccacaagc agctcaaacg cctgcggaga gttatcgcct
    25921 ggaccgggga acgggaaacg cctgtcacca tttatagctg ggtcgcggcg gcaacgcgtt
    25981 atcagcttct caagcgcggc gttatcagta acacaaaaat caacgccacc gaagaagaga
    26041 tcctgcaagg cgaaccggaa gtaaaagtag agtcagccga acgtcatcat gcaatggtta
    26101 acttctggcg aaccacactt tcctgcattc tgggcacgct tttctggctg tggacgggct
    26161 ggacttccgg cagtggtgca atggtgatga ttgcggtcgt gacgtcactg gcaatgcgtt
    26221 tgccgaatcc acgcatggtg gcgatcgact ttatctatgg gacgctggcc gcgctgccgt
    26281 tagggctgct ctactttttg gtgattatcc ctaataccca acagagcatg ttgctgctgt
    26341 gtattagcct ggcagtgctg ggattcttcc tcggtataga agtacagaaa cggcgactgg
    26401 gctcgatggg ggcactggcc agcaccataa atattatcgt gctggataac ccgatgactt
    26461 tccatttcag tcagtttctc gacagcgcat tagggcaaat cgtcggctgt gtgctcgcgt
    26521 tcaccgttat tttgctggtg cgggataaat cgcgcgacag gactggacgt gtactgctta
    26581 atcagtttgt ttttgccgct gtttccgcga tgactaccaa tgtggcacgt cgtaaagaga
    26641 accacctccc ggcactttat cagcagctgt ttttgctgat gaataagttc ccaggggatt
    26701 tgccgaaatt tcgcctggcg ctgacgatga ttatcgcgca ccagcgcctg cgtgatgcgc
    26761 cgatcccggt taacgaggat ttatcggcgt ttcaccgaca aatgcgccgc acagcagacc
    26821 atgtgatatc tgcccgtagc gatgataaac gtcgtcggta ctttggccag ttgctggaag
    26881 aactggaaat ctaccaggaa aagctacgca tctggcaagc gccaccgcag gtgacggaac
    26941 cggttcatcg gctggcgggg atgctccata agtatcaaca tgcgttgacc gatagttaag
    27001 tcaaaaccga cgccaaaagc gtcggttttt tcatggctat acttagcgat gcacggcaga
    27061 actcgccgcg aaacgtgacg gtggcaacag atgaatattt atacctttga ttttgatgag
    27121 attgagagtc aggaggattt ttatcgtgac tttagccaaa cctttggtct ggctaaagat
    27181 aaggtacgcg atctcgactc actatgggat gtgttaatga acgatgtcct gccgctacca
    27241 cttgagattg aatttgttca tctgggagag aaaacgcgtc gccgttttgg cgcgttaata
    27301 ttgctgtttg atgaggcaga ggaagagctg gaagggcatt tgcgttttaa tgttcgtcat
    27361 tagcgcaaaa aaaagccccc gaaccggggg caatatcgtc ggacaagacg atgagggttt
    27421 atttgtacag ttcagccgta gcgtgccagg tgtcaccgct acgagcttca gtaatctggt
    27481 aggccgttgc gcctttctct tccgcttttt tgttcagcat ttcacgcata tccattggcg
    27541 aagacgccac accacttacg gatacggtcc cgattgcttc acgattttgt gcttgtgcag
    27601 tatcaatgga gtcggcagcg aatgcaccga aagagagaac agaaagtacg cttaatgcag
    27661 ctacagtggt tttgattttc atgattttta cctcgacata atcttttagc tgggcctttg
    27721 tttcgtgatc catatcacaa aatcaagcat acactaatta ttgaactaat taataccaca
    27781 ctaa
//
LOCUS       DADMKF010000045        27105 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.046, whole genome shotgun sequence.
ACCESSION   DADMKF010000045 DADMKF010000000
VERSION     DADMKF010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27105
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.046"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            <1..924
                     /locus_tag="JNC24_001727"
     CDS             <1..924
                     /locus_tag="JNC24_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY9158485.1"
                     /translation="TSATLSSTNWQYACSCSAGKAVKLVYMVSPVLTTTGHQTGYYKL
                     NDSLDIKTTLQANDIPGLTTDQVVSVNTRFTQIKSSTVYSAATQTGVCQGDTSRYGPV
                     NIGANTTFTLYVTKPFLGSMTIPKTDIAVIKGAWVDGMGSPSTGDFHDLVKLSIQGNL
                     TAPQSCKINQGDVIKVNFGFINGQKFTTRNAMPDGFTPVDFDITYDCGDTSKIKNSLQ
                     MRIDGTTGVVDQYNLVARRRSSDNVPDVGIRIENLGGGVANIPFQNGILPVDPSGHGT
                     VNMRAWPVNLVGGELETGKFQGTATITVMVR"
     gene            complement(967..1827)
                     /gene="rsmI"
                     /locus_tag="JNC24_001728"
     CDS             complement(967..1827)
                     /gene="rsmI"
                     /locus_tag="JNC24_001728"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HAY9158486.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRALEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            1891..3927
                     /locus_tag="JNC24_001729"
     CDS             1891..3927
                     /locus_tag="JNC24_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HAY9158487.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAVEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKKQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTVDGVASPAQASVSDLTGDQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSSLGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGTPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGAML
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPVS"
     gene            3885..4280
                     /locus_tag="JNC24_001730"
     CDS             3885..4280
                     /locus_tag="JNC24_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HAY9158488.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEAQARRWLEGKGLRFIAANVN
                     ERGGEIDLIMREGRTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            4300..4890
                     /gene="diaA"
                     /locus_tag="JNC24_001731"
     CDS             4300..4890
                     /gene="diaA"
                     /locus_tag="JNC24_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HAY9158489.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            4900..5475
                     /gene="yraP"
                     /locus_tag="JNC24_001732"
     CDS             4900..5475
                     /gene="yraP"
                     /locus_tag="JNC24_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divisome-associated lipoprotein YraP"
                     /protein_id="HAY9158490.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKETRINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(5589..6629)
                     /locus_tag="JNC24_001733"
     CDS             complement(5589..6629)
                     /locus_tag="JNC24_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HAY9158491.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQSGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(6702..7337)
                     /locus_tag="JNC24_001734"
     CDS             complement(6702..7337)
                     /locus_tag="JNC24_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HAY9158492.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLSDALAQVTEPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            7465..7983
                     /gene="yhbO"
                     /locus_tag="JNC24_001735"
     CDS             7465..7983
                     /gene="yhbO"
                     /locus_tag="JNC24_001735"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HAY9158493.1"
                     /translation="MSKKIAVLISDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGAEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(7963..8406)
                     /locus_tag="JNC24_001736"
     CDS             complement(7963..8406)
                     /locus_tag="JNC24_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HAY9158494.1"
                     /translation="METLITISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARQAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRDSGTEQA"
     gene            8457..8759
                     /gene="yhbQ"
                     /locus_tag="JNC24_001737"
     CDS             8457..8759
                     /gene="yhbQ"
                     /locus_tag="JNC24_001737"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HAY9158495.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPEIKSD"
     gene            complement(8746..9249)
                     /locus_tag="JNC24_001738"
     CDS             complement(8746..9249)
                     /locus_tag="JNC24_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAY9158496.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(9243..9767)
                     /locus_tag="JNC24_001739"
     CDS             complement(9243..9767)
                     /locus_tag="JNC24_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HAY9158497.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNAQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGLYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            9976..10971
                     /locus_tag="JNC24_001740"
     CDS             9976..10971
                     /locus_tag="JNC24_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAY9158498.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            10980..11858
                     /locus_tag="JNC24_001741"
     CDS             10980..11858
                     /locus_tag="JNC24_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAY9158499.1"
                     /translation="MKYSLGPVLWYWPKEALEEFYQQAATSSADVIYLGEAVCSKRRA
                     TKVGDWLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELTSMQGLVDVVRLSPQGTDTFAMLDAFRANENGAAPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            12064..13071
                     /locus_tag="JNC24_001742"
     CDS             12064..13071
                     /locus_tag="JNC24_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="luciferase-like monooxygenase"
                     /protein_id="HAY9158500.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYGV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(13189..14433)
                     /gene="mtr"
                     /locus_tag="JNC24_001743"
     CDS             complement(13189..14433)
                     /gene="mtr"
                     /locus_tag="JNC24_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HAY9158501.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSALGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMITLIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(14587..16476)
                     /locus_tag="JNC24_001744"
     CDS             complement(14587..16476)
                     /locus_tag="JNC24_001744"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH family ATP-dependent RNA helicase"
                     /protein_id="HAY9158502.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(16469..16549)
                     /gene="yrbN"
                     /locus_tag="JNC24_001745"
     CDS             complement(16469..16549)
                     /gene="yrbN"
                     /locus_tag="JNC24_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HAY9158503.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(16656..17540)
                     /gene="nlpI"
                     /locus_tag="JNC24_001746"
     CDS             complement(16656..17540)
                     /gene="nlpI"
                     /locus_tag="JNC24_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HAY9158504.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(17649..19784)
                     /gene="pnp"
                     /locus_tag="JNC24_001747"
     CDS             complement(17649..19784)
                     /gene="pnp"
                     /locus_tag="JNC24_001747"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HAY9158505.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSRDGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(20031..20300)
                     /gene="rpsO"
                     /locus_tag="JNC24_001748"
     CDS             complement(20031..20300)
                     /gene="rpsO"
                     /locus_tag="JNC24_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HAY9158506.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(20449..21393)
                     /gene="truB"
                     /locus_tag="JNC24_001749"
     CDS             complement(20449..21393)
                     /gene="truB"
                     /locus_tag="JNC24_001749"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HAY9158507.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERMVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(21393..21794)
                     /gene="rbfA"
                     /locus_tag="JNC24_001750"
     CDS             complement(21393..21794)
                     /gene="rbfA"
                     /locus_tag="JNC24_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HAY9158508.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(21958..24630)
                     /gene="infB"
                     /locus_tag="JNC24_001751"
     CDS             complement(21958..24630)
                     /gene="infB"
                     /locus_tag="JNC24_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HAY9158509.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQAQREAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSN
                     QQDDMTKNAQAEKARREQEAAELKRKAEEEAHRKLEEEARRVAEEARRMAEENKWTDN
                     AEPTEDSSDYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADRE
                     EARAAVRGGKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIK
                     AMMKLGAMATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAP
                     VVTIMGHVDHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAA
                     FTSMRARGAQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPD
                     RVKNELSQYGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMAS
                     GAVIESFLDKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSI
                     PVEILGLSGVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMT
                     EGEVHEVNIVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASN
                     AILVGFNVRADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLA
                     EVRDVFKSPKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVR
                     NGMECGIGVKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(24655..26142)
                     /gene="nusA"
                     /locus_tag="JNC24_001752"
     CDS             complement(24655..26142)
                     /gene="nusA"
                     /locus_tag="JNC24_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HAY9158510.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(26170..26622)
                     /gene="rimP"
                     /locus_tag="JNC24_001753"
     CDS             complement(26170..26622)
                     /gene="rimP"
                     /locus_tag="JNC24_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HAY9158511.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(26829..26905)
                     /locus_tag="JNC24_001754"
     tRNA            complement(26829..26905)
                     /locus_tag="JNC24_001754"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(26869..26871),aa:Met,seq:cat)
BASE COUNT         6419 a         7518 c         6812 g         6356 t
ORIGIN      
        1 acctctgcaa cgctaagtag cactaactgg caatacgcct gttcctgctc tgcgggtaag
       61 gcagttaaac ttgtctatat ggtcagcccc gtacttacca ccactggaca tcagacagga
      121 tattacaaac tcaatgacag cctggatatt aaaaccacat tacaggcaaa cgacattcca
      181 ggactcacaa ccgaccaggt tgtctctgtt aacacccgat tcacacagat aaaaagcagc
      241 accgtatatt ctgctgcaac ccaaacgggt gtttgccagg gtgacacgtc tcgttatgga
      301 cccgttaata ttggtgcaaa taccaccttt accctgtatg tcaccaagcc atttctcggc
      361 tcgatgacca ttccgaaaac ggatattgcc gtcattaaag gcgcgtgggt cgatggaatg
      421 ggaagcccgt ctacaggtga cttccatgat ttagtcaagt tatcgattca gggaaatctc
      481 accgccccac agtcgtgcaa aattaatcag ggcgatgtta ttaaggttaa ttttggattc
      541 atcaatggtc agaagtttac cacccgcaat gccatgccag acggttttac tccagtagac
      601 tttgatatca cttatgactg tggtgatact tcaaagatta aaaactcgtt gcaaatgcgc
      661 atcgacggta caactggggt agtagaccag tacaacctgg tcgccaggcg aagaagttca
      721 gacaatgtgc ccgatgtcgg tattcgtatt gaaaatctcg gcggcggagt tgcaaatatt
      781 ccttttcaga acggtatcct tcccgttgat ccttccgggc atggcaccgt caatatgcgc
      841 gcctggccag ttaatctggt cggtggtgag ctggaaacag gaaaatttca gggcaccgcc
      901 accattaccg tcatggtgcg gtaaaagaag acgttgcgtt ttgattaacg caacgcttcg
      961 gcactgttac ccctgctgct ccagcgcata cttatacagc gcatttttct tcacgccatg
     1021 aatttctgcg gccagcgccg ccgctttttt cagcggcagt tctgcctgta gcagcgccag
     1081 cgtgcgcagg gcatcggcgg gtaagtcttc ttcctgtgct ttatgacctt cgacaatcag
     1141 caccatttcg cctttgcgac ggttttcatc ttcctttacc cacgccagca gctcgccaac
     1201 gggcgcgccg tgaatggttt cccaggtttt ggtcagctca cgcgccagaa ccacgtagcg
     1261 ggattcgcct aataccgcaa cgatatcttc caggctatct aacagacggt gggtagattc
     1321 ataaaaaatc agcgtgcgcg gctccgcttc aatggctttt agcgcatcac ggcggccttt
     1381 tgatttggca ggtaaaaagc cttcgtaaca gaaacggtca gagggtaaac ccgcggcgct
     1441 taacgcagtg atagcagcac acggtccggg tagcggcacc acacgaatcc ccgcttcacg
     1501 gcaggtacgc accagatggt agccaggatc gttaattagc ggcgttccgg catcggaaac
     1561 cagcgcaatg ttttgcccct cttgcagctt cgccagcagc gtttcggctt tttgttgttc
     1621 gttatggtcg tgcagcgcaa acaaccgggc attaatccca aaatgttgca gcaataaacc
     1681 ggtgtgacga gtatcctcgg cggcaatcag atcaacggcc tgtaatacct ctaacgcacg
     1741 ctgggtgata tccgccagat tgccgattgg cgtcggtaca atgtaaagct ggccctgaga
     1801 attatccgcc gattggtgtt gtttcattgt gtcgtccgta ttgccgattt aatattgagc
     1861 attgcgtaaa aaaatatcac tggatacatt atggtaccct caacattttc tcgtttgaaa
     1921 gccgcgcgtt gtctgcctgt tgttctggca gccctgattt tcgccggttg tggcacccat
     1981 actcccgatc agtccactgc ttatatgcag ggcacggcgc aggctgattc tgccttttat
     2041 cttcagcaga tgcagcaaag ctctgatgat accaggatca actggcaatt actcgccatt
     2101 cgtgcactgg tgaaagaagg taaaaccggg caggcggttg agttgtttaa ccaactaccg
     2161 caagaactga acgattctca gcgtcgcgag aaaacactgc tggcggtaga gattaaactg
     2221 gcgcagaaag attttgctgg cgcgcaaaac ttgctggcga aaatcacacc tgccgattta
     2281 gaacaaaacc agcaagcgcg ttactggcag gcaaaaatcg atgccagcca ggggcgtcct
     2341 tccattgatt tactgcgcgc gttaattgct caggaaccgc tgctcggcgc gaaagaaaaa
     2401 aagcagaata ttgatgccac ctggcaggcg ctctcctcca tgactcagga acaggcgaat
     2461 acgctggtga tcaacgccga cgaaaatatt ctgcaaggct ggctggatct gcagcgcgtc
     2521 tggtttgata accgtaacga tcccgacatg atgaaagccg ggatcgccga ctggcaaaaa
     2581 cgttatccga acaatccggg cgcgaaaatg ctgccaacgc agttggttaa cgtaaaagcg
     2641 tttaaaccag cctcgaccaa caaaatcgcc ctgctgttgc cgctgaatgg ccaggcagcg
     2701 gtatttggtc gcaccattca gcaaggcttt gaagcggcga aaaatatcgg cactcagcca
     2761 gtggcggctc aggtagctgc cgcacctgcc gcagacgtag ctgaacaacc tcagccgcaa
     2821 accgtggatg gcgttgccag cccggcacaa gcctcggtta gcgatctgac cggtgatcag
     2881 cctgcggccc agccggtgcc tgtaagcgcc ccggcgacaa gcaccgcagc ggtaagcgca
     2941 cccgcaaatc catccgcaga gctgaaaatc tacgatacct catcacaacc acttagccag
     3001 atcttaagcc aggttcagca ggatggcgcg agtattgtgg ttggtccgtt gctgaaaaat
     3061 aacgttgaag agttgctgaa gagcaacacc ccgctgaacg tgctggcact gaaccagcca
     3121 gagaatatcg aaaaccgcgt caatatttgt tacttcgcgc tttcacctga agacgaagcg
     3181 cgcgatgcag cgcgtcatat tcgtgaccag ggtaaacaag cgccgctggt gctgatccca
     3241 cgcagttcat tgggcgatcg cgtagccaat gcgtttgcgc aagagtggca gaaactgggc
     3301 ggcggcaccg ttctgcaaca aaaatttggt tccaccagcg aattacgcgc gggtgttaac
     3361 ggcggttctg gtatcgcttt aacgggtacc ccgattactc ccagagcgac aaccgactcc
     3421 ggcatgacga ccaacaatcc aacgctgcaa accacgccaa ccgatgacca gttcaccaat
     3481 aatggcggtc gtgtcgatgc ggtgtacatt gtggcaacgc cgggtgaaat cgcttttatc
     3541 aaaccgatga tcgccatgcg taacggtagc cagagcggtg caatgctgta cgccagctcc
     3601 cgcagcgcac aaggcaccgc aggcccggat ttccgtctgg agatggaagg tttgcagtac
     3661 agcgaaatcc cgatgctggc gggcggtaat ctgccgttaa tgcagcaggc actcagcgcg
     3721 gtgaataacg attattcact ggctcgcatg tatgcgatgg gcgtcgatgc ctggtcgctg
     3781 gcaaatcatt tctcacaaat gcgccaggtt cagggttttg aaatcaacgg taataccgga
     3841 agcctgacgg caaacccgga ttgcgtgatt aacaggaagt tatcatggct acagtaccaa
     3901 caaggtcagg tagtccccgt cagttaacca ccaaacagac cggcgatgcg tgggaagcac
     3961 aagcgcgtcg ctggctggaa ggcaaaggac tgcggtttat cgccgctaac gtgaacgagc
     4021 gtggcggcga gatcgatctg ataatgcgtg aaggccggac caccgttttt gtcgaggtac
     4081 gctatcgccg ctctgcgctt tatggcggcg cggcagccag tgtgacccgc agcaaacaac
     4141 acaaattatt acagactgcc cgcttgtggc tcgcgcgtca taatgggagt tttgatactg
     4201 tggattgccg gttcgatgtg gtagccttca ccgggaatga ggttgagtgg attaaggatg
     4261 cctttaatga ccactcataa ttaaggttta aggattagcg tgcaagaaag aattaaagct
     4321 tgcttcactg aaagcattca aactcaaatt gcggcggcag aggcgcttcc ggatgccatc
     4381 tcccgtgcag ccatgacgct ggttcagtct ctgctcaatg gcaacaaaat cctctgttgt
     4441 ggtaatggaa cttccgctgc caatgcacag cattttgctg ccagcatgat caaccgtttc
     4501 gaaacggagc ggcccagctt acctgccatt gcactaaata ctgataatgt tgtcttaacg
     4561 gcgattgcca acgatcgctt acatgatgaa gtgtatgcaa aacaggtgcg ggcgctgggt
     4621 catgcgggag atgtattgtt agccatttcc acccgtggca acagccgcga tattgttaaa
     4681 gcagttgaag ccgccgttac gcgtgatatg accattgtgg cattgaccgg ctatgacggc
     4741 ggcgagcttg caggtttgtt agggccacag gatgtggaga tccgcattcc ttcgcatcgt
     4801 agtgctcgca ttcaggaaat gcatatgctg acggtaaatt gcctgtgcga tctgatcgat
     4861 aacacgcttt tccctcacca ggatgattaa ggagaataca tgaaggcatt atcgccaatc
     4921 gcagtcctta tttccgcgct gctgttgcaa ggttgtgttg ccgctgccgt agtgggtacc
     4981 gctgctgtgg gtaccaaagc cgcaactgac ccacgcagtg tcggcaccca ggtggacgat
     5041 ggtaccctgg aagtgcgcgt gaacagcgca ttgtcgaaag acgaacagat taagaaagaa
     5101 acccgtatta atgtcacggc gtatcagggc aaagtgctgc tggttgggca gtcaccaaac
     5161 gctgaacttt cggctcgcgc caaacagatt gctatgggcg tagacggtgc caacgaagtg
     5221 tataacgaga ttcgtcaggg ccagccgatt ggtctgggcg aagcatctaa cgatacgtgg
     5281 atcaccacca aagtgcgttc gcagctctta accagcgacc tggtgaaatc gtccaacgtg
     5341 aaagtgacca ccgaaaacgg tgaagtgttc ctgatggggc tggtgactga acgtgaagcg
     5401 aaagcggcgg cagatattgc cagccgggtg agcggcgtaa agcgggtaac cacagcgttt
     5461 acgtttatta aatagcagcc ccttgtaatg cctgatgcga cgcttgccgc gtcttatcag
     5521 gcctacaacg atgcaaaccg taggtcggat aaggcgttta cgccgcatcc gacatgtttt
     5581 ccctcaaatc agaataacag cgccagcccg ccgacaatca cgcctgacac tgccaccatc
     5641 gcccccgtca gccataaggc tttcgccggg aacgctttgc gcagcataat cagtgacggc
     5701 aaactcactg ccgggagcgt catcaacaat gccagcgccg gagcggttcc catacctgcc
     5761 agcatcatcg tttgtacaat cggaatttct gctgccgtgg gaatgacaaa caagcatcct
     5821 gctaccgcca tcgccaccac ccacatcagg ctgttatcga cagcaccatc ggcatggggg
     5881 aataaccaga cgcgtgcggc acccaacacc agtactgcaa ggatgtaaac cgggatcgta
     5941 ctccagaaaa gtgtccatag cgccctgccc cagcggctaa aaaaaccgct ctgtgcttcc
     6001 ggtatgtcaa tttcgaccgg tgcctgcgtt tgcggtgttt cacgcaccca tttttgcacc
     6061 agcgtcgcaa tcagcaacac catcaccagc ccggccacca gacgaatcgc cgcaaaaccc
     6121 cagccgagga caaaccccat aaacaccagc gtcgccgggt ttaacaccgg attgcccatc
     6181 cagaatgcca gcgcaccgcc catcgatacc tgttggcgac gcattcccgc cgcgaccgga
     6241 gccgcacagc aggtacacat catgcccggc agcgaaaaca gcgttcccag cagcgtgccg
     6301 cgaaagcgcg attgtccaag cgtacgcaac aaccagtcac gcgggatcag cacctgaatc
     6361 aacgaaccga ggatcacccc cagcaccgcc gctttccata ccgcgaggaa atagatcatc
     6421 gcgtaatcca acgccgcctg ccacggatta gcatccgcct gcgcaaggat agatttaccg
     6481 atactgtggg tttcggcagc agtaaacgct ttgccgtagt aaggttgcca tttcacatac
     6541 cagagaccgg caatgacaac gagaaagaaa agcgcgggtt tccaccactg aatgggcgtt
     6601 gccgcctgag atgaagactg accagtcata gcattcccca ggaataagtt atgataattg
     6661 agcgcgtgaa tattacgctc actatcaatt cttgggaata attattcagc tcttttgcgt
     6721 aattctgaag agcttaaaat agtcacgcct tcatgctcag gccgcatcgc ctctgccagc
     6781 atgacccgcg ccacgtctct cgcatcaatg gatttccagt taccgggcag taaacgaaac
     6841 agcggcgcaa aaagcgtttc gttcatccgc tgtttgctac gatcgcccag caacatcgac
     6901 gggcgagcaa tggtcaattt cggccagttc tgggcaatta atgcttcttc catctcccct
     6961 ttgacgcggt tatagaaaaa cggcgagtgg gcattggcac ccatagcact gaccaccagc
     7021 atatgctgcg cgcccagtcg ccgcccggtt aatgcggtat ccactaccag cgtgtaatcg
     7081 gcatgaataa acgcctcttt gctccccgct tctcgccgcg tggtgccgag acaacaaaac
     7141 acaatgtcga tgggttcggt gacctgcgcc aacgcgtcac tcagttgcgg atcatgggga
     7201 ttaaacaccc cgggcatatc gcccaacgga cgtcgcgtcg gcgcggctat ggcgttaact
     7261 ttcggttcgt taatcaacat ccgcagcagg tgaccgccca ccagccccgt tgcgcctgta
     7321 atcagtacct gactcatctt cgctccttta cagaattgtc tgccttgcgc tccacggctc
     7381 atgcaccagg cttaatagac catgaggtaa ttatccccga ttgtgtgaaa ttcgcctcat
     7441 ccaatgcaac aacgcggagg aagcatgagt aagaaaattg ccgttttaat cagtgatgaa
     7501 tttgaggatt cagaatttac ttcacccgca gacgagttcc gtaaagccgg acacgaagtg
     7561 attaccattg aaaaacaagc gggtaaaacg gtgaaaggca aaaaaggaga agccagcgtg
     7621 accatcgata aatccatcga tgaagtgacg ccagcggagt ttgatgccct gctgctaccg
     7681 ggtggtcatt caccggatta tctgcgtggc gacaaccgtt ttgtcacctt tacccgtgat
     7741 tttgtgaata gcggcaaacc tgttttcgcc atctgtcacg gtccgcagct gctgatcagc
     7801 gccgatgtga ttcgggggcg caaactgacc gcagttaaac cgatcattat tgatgttaaa
     7861 aatgcgggcg cggaatttta cgatcaggaa gtcgtggttg ataaagatca gctggttacc
     7921 agccggacac cggacgatct gccagcgttt aaccgcgagg cgttacgcct gctcggtgcc
     7981 tgagtcgcgt agccaaatca tttttttacc aaagcccagc gtgttgtcgg tgaatttgat
     8041 ttcatcgagc cggatttccc acaccggggc cgacagcatt ctggcaaccg gaaagcgacg
     8101 attgtacgcc tggcgcgcga ggtcgctttc ctcaccttcc agcctgcgga tctcaccttt
     8161 aaactgcacg ccgcgaatta acgctaccgt tttcggctga ccgtttaccg ttccggcaac
     8221 cgcagcctgc ggcccactca tctgcgcgtg gcgcgttttt tcttccgtca aaatgtagaa
     8281 ggcgactttc tgcgcatcaa acagataaaa agcattagcg caccacagtt ccccttcctg
     8341 ctgcacacac caggtgacaa catgttgttt tgccaaccaa cggctgatgg tgatgagtgt
     8401 ttccattgct gctctctttt atactgtggg cctgacctta acatgcgacg cagacgatga
     8461 caccctggtt tctttacttg atccgtaccg ccgacaataa gctttatacc gggatcacca
     8521 cggatgtcga acgccgctat cagcagcacc aaagcggcaa aggggcgaaa gcactgcgcg
     8581 ggaaaggaga actgacgctg gcgttttctg cgccagtggg cgatcgttcg ctggcgttac
     8641 gggcggaata tcgcgttaag caactgacca aacggcaaaa agagcgtctg gtagcggaag
     8701 gcgcagggtt tgcagagctg ttaagcagtc tgcaaacccc ggagattaaa agcgattgaa
     8761 atgctcgtga tactcaacca gaccggttac gccattcagc gcgtcatccg ctaaacgatg
     8821 tacctggaag gcgctttcag tgcccggcca gcggcaacgc agatcgtgat gcgccgccag
     8881 ttcaaagccg aaacgactgt acagcgccgg atcgcccagc gtcaccactg cggcatagcc
     8941 gaactcatta agcgaatcga gtccttcata gaccagttgg cgtgccagcc cctgtccacg
     9001 gtatttttca tcaaccgcca acggtgccat gccgacccat tgcaggtctt cgccctgcac
     9061 atcaaccgga ctaaatgcca catagccaat gacctgacct tcgtcatctg tcgccaccag
     9121 ccccagcgtc agaaagccat cttcacgcag atcgtgaacc agcttcgctt ccgcatcact
     9181 ttcgaatgag cgacgcagca gggcatcaat acccggcgca tcaatgggaa tttctactcg
     9241 aattagcatg gttcacctac cgatgtctgt ttggtttcag gcgcggtttt cattcccgcc
     9301 tcaacaaaat ccgccagttg cagcagcatc atgcgtaagg ctttcggcat ttgctccagt
     9361 tcaatggcgt ccatcaggtt tttcacatac agccccagct ccgtatcgcc ttcaatcacc
     9421 agccgacgct ggaagaagag cgtatccgga tcttgtttac gcgccgcaat catcagcaga
     9481 tcgctggcgt cggcactaaa actcacatca gcttgcgcgt tctggctgac caccagtttg
     9541 ccattcacca ccgaggtaaa ccattgcagg tcaatatcac gcacatgaat acttaaccag
     9601 cggccttcaa gaaactccag ctcaccatca tccagcgcct gacggaattg ccagcttaag
     9661 acctgctcaa gaacctggcg ttttagcgca aatggcgtca gttttaccgg tacactcaac
     9721 agagatggcc ccaaatgcac aatacgggaa cgcagtttat ccaacacgag ttttactccc
     9781 tgtttcaaca atcatcctat tttgccatat cagaaaaata acatagcggt ataaatcaac
     9841 aattccatat gaaattgctg ctaccaccaa tacaacttta actgccttaa atcaaaaatt
     9901 gtcgcagcaa ggttaactaa aatcccagtt cgttaacatt tttgcgtttt gaaagcgcaa
     9961 ccttcaggaa aaattatgga gctgctctgc cctgccggaa atctcccggc gcttaaggcg
    10021 gccatcgaaa acggcgcaga tgctgtttat atcgggctaa aagatgatac taatgcccgt
    10081 cacttcgccg gccttaactt taccgagaaa aaattgcagg aagcggtgag ttttgtccat
    10141 caacatcgcc gcaaacttca catcgcgatt aacacttttg cgcatccgga cggttacgcc
    10201 cgttggcagc gcgccgtgga tatggcggcg cagctgggtg ccgacgcgct gatcctcgcc
    10261 gacctcgcca tgctggagta cgccgccgag cgttacccgc atattgagcg tcatgtgtca
    10321 gtgcaggctt cggcgaccaa tgaagaggcg attaactttt atcatcgcca ttttgatgtt
    10381 gcccgcgtgg tgctgccgcg cgtgttgtcg attcatcagg tgaaacagct ggcacgggtc
    10441 acacctgtcc cgctggaagt ctttgctttt ggcagcctgt gcattatgtc ggaaggtcgt
    10501 tgctatctgt cgtcgtatct gacgggtgag tcgcccaaca ccgtgggcgc gtgttctccg
    10561 gcccgtttcg tgcgctggca acaaacgccg caggggctgg aatcccgcct gaacgaagtg
    10621 ctgatcgacc gttatcagga tggcgaaaac gcaggttatc cgacgctgtg taaagggcgt
    10681 tatctggtgg acggcgagcg ctatcacgcg ctggaagaac caaccagtct caataccctg
    10741 gaactgctgc cggagttaat ggcggcgaat attgcttcgg tgaaaattga aggccgccag
    10801 cgtagcccgg cgtatgtcag ccaggtggcg aaagtctggc gtcaggctat cgaccgttgt
    10861 aaggccgatc cgcaaaactt cgtaccgcaa agcgcgtgga tggagacgct cgggtcgatg
    10921 tccgaaggca cgcagaccac ccttggcgcg tatcaccgta aatggcagtg agaagagcaa
    10981 tgaaatattc cttagggcca gtgctgtggt actggccaaa agaggcgctg gaagaatttt
    11041 atcagcaggc cgccaccagc agcgccgacg tgatttatct tggtgaagcg gtatgcagca
    11101 agcgccgggc aaccaaagtt ggcgactggc tggagatggc aaaatcgctc gccggaagcg
    11161 ggaagcagat agtgctctcc acgctggcgc tggtgcaggc atcctctgaa ctgggcgaac
    11221 tgaaacgcta tgttgagaac ggtgagtttc tgattgaagc cagcgatctc ggcgtggtga
    11281 atatgtgcgc tgaacgcaaa ctgccgttcg tcgccgggca cgcgctgaac tgctacaacg
    11341 cggtgacgct gaaaatattg ctcaaacagg gcatgatgcg ctggtgtatg ccggtggagc
    11401 tttcccgcga ctggctggtg aatctgctta atcagtgcga tgagttgggg attcgcaacc
    11461 agtttgaagt ggaagtcctg agctacggtc atctaccgct ggcctactcc gcccgctgct
    11521 ttaccgcgcg ttcggaagac cgcccgaaag atgagtgtga aacctgctgc attaagtatc
    11581 cgaacgggcg caacgtgctg tcgcaggaaa accaacaagt gtttgtactc aatggcattc
    11641 agaccatgag cggctacgtt tacaacctcg gtaacgagct gacatccatg cagggattgg
    11701 ttgatgtggt ccgcctgtca ccgcagggta ctgacacttt cgcgatgctc gacgccttcc
    11761 gcgctaatga aaatggcgct gcgccactgc cgttgacggc aaacagcgac tgtaacggct
    11821 actggcggcg gctggctggg ctggagctgc aagcctaagt aaatatgctc gtcatacttc
    11881 aagttgcatg tgctgcatct gcattcgttc accccagtca cttacttatg taagctcctg
    11941 gggattcact ctcttgtcgc cttcctgcaa ctcgaattat ttagagcaca gctcactttg
    12001 ttaacaactt taactactct ttaatgcagt attaaagatt aatcggtaac aaagtgagct
    12061 gttatgactg ataaaaccat tgcgttttcg ctactcgatc tggcccccat tcccgaaggt
    12121 tcttcagcgc gagaagcatt ctcccactct ctcgatctcg cccgtctggc tgaaaagcgc
    12181 ggctatcatc gctactggct ggcagaacac cacaatatga ctggcattgc cagtgctgcc
    12241 acgtcggtat tgattggcta tctggcggcg aataccacca cgctgcatct ggggtctggc
    12301 ggcgtgatgt tgcctaacca ctcaccgttg gtcattgccg aacagttcgg cacgctcaat
    12361 acactctatc cggggcgaat cgatttgggg ctgggtcgtg cgccaggtag tgaccaacgg
    12421 acaatgatgg cgctgcgtcg ccatatgagc ggcgatattg ataatttccc ccgcgatgtc
    12481 gcggagctgg tggactggtt tgacgcccgc gatcccaatc cgcatgtgcg cccggtacca
    12541 ggctatggcg agaaaatccc cgtgtggttg ttaggctcca gcctttacag cgcgcaactg
    12601 gcggcgcagc ttggtctgcc gttcgcgttt gcctcacact tcgcgccgga tatgttgttc
    12661 caggcgctgc atctttatcg cagcaacttc aaaccgtcgg cacgactgga aaaaccatac
    12721 gcgatggtgt gcatcaatat tatcgccgcc gacagcaacc gcgacgctga atttctattt
    12781 acctcaatgc agcaagcctt tgtgaagctg cgccgtggcg aaaccggaca actgccgccg
    12841 ccgattcaaa atatggatca gttctggtca ccgtctgagc agtatggcgt gcagcaggcg
    12901 ctgagtatgt cgttggtggg tgataaagcg aaagtgcgtc acggcttgca gtcgatcctg
    12961 cgcgaaaccg acgccgatga aattatggtt aacgggcaga ttttcgacca ccaggcgcgc
    13021 ctgcattcgt ttgaactggc gatggatgtg aaggaagagt tgttgggata gtgtgtctta
    13081 acgcgggaag ccttatccga gctggcaacg ctgtcctacg tagacctgat aagcgaagcg
    13141 catcaggcat tgtgtaggca gcagaaatgt cggataaggc accgctgatt actgatacac
    13201 cggcagcaaa ttaaagctcg ataagatatg gaccagtgcg ttgccaacgc caaacaccag
    13261 aatcagcgta atcatcggct tgccgcccca gacacggaat ttcgggctgc caaagcgttt
    13321 acgcgatgcg cgggctaaca gcgccggaac aattgccgcc cagatggtag ctgctaaacc
    13381 agcataacca atggcgtaca ggaatccgtt cgggaacagt agaccaccca caacaggcgg
    13441 ggcaaaggtc agcaatgccg ttttcaagcg ccccagggct gagtcatcga aaccaaacag
    13501 atctgccaga tagtcaaaca aacccagcgt tacgccaagg aacgaactcg ctaccgcaaa
    13561 gtttgagaac acgaccagca gaaggtccag gctacggctg ttcagcacgc cgcttaatgc
    13621 ctgtaccagc acatcaatat taccgccctt ctctgcaata ccgataaact ccggacgcgg
    13681 gatgttaccc atcgtcgcca gcaaccagat ggtatacagc gccagtgcca tcagcgtacc
    13741 gtacaccagg catttcacga tggttttcgg atctttgccg taatacttca tcaggctcgg
    13801 cacgttaccg tgataaccaa acgatgccag acagaacggc agggtcatca gcaaatacgg
    13861 cgcataagat gcattgcttt cggcgacgtt gaacaatgtc gcaggctgca catgccccag
    13921 taggctaccg aaggtgagga agaaggtaat gactttcgcc cccagtacaa tcgccgtcat
    13981 gcggctgacg gccttagtac tcaaccacac cacaaacgct accagcagtg caaaaccaaa
    14041 acccgccgcc cgtgccggga cgtttagcga catctctgcg aaggtgtgat gcagaattga
    14101 accactggca gaaatatagg cataggtcag gatatagagc acaaaggcaa tggaaatacc
    14161 gttgaccacg ttccagcctt tgcccagcaa atctttggtg atggtgtcaa aactcgaacc
    14221 gattcggtaa ttcaggttag cttccagaat catcaagccg gaatgcagca tacagaacca
    14281 ggtaaagatc agcgccgcca ttgaccagaa aaaccacgcc ccggacatga ccactggcag
    14341 agaaaacatc cctgcgccaa taatggtgcc gccgataatc accacgccgc caagcagcga
    14401 cggtgacgtt tgggtggtgg ttagtgttgc catgagagct tctctccagt gaaaaatagt
    14461 gcgactgcgt tgttatgcat tgcactgtac cagtacacga gtacaaaaga cagaaaaaaa
    14521 gccccgatgg taaaaatcgg ggctgtatat attattttac agattgtgtt cgctgttcag
    14581 cgatgattac gcatcaccac cgaaacgacg acgaccggta gagtcatcac gacgcggagc
    14641 gcggccttca cgacgttcgc cgctaaaacg acgaccatca ccacggccac cttcacggcg
    14701 ttcaccgctg aagttacgac cgccttcacg acgttcgcca ccgaaaccac gaccaccgcc
    14761 acgacgctca ccgccagtat gcggctgtgc atcgcccagt aactgcatgt tcatcggctt
    14821 gttgagaatg cgagtgcgcg taaagtgttg cagcacttca cccggcatac ctttcggcag
    14881 ttcgatggtg gagtgagaag caaacagctt gatgttacca atgtaacggc tactgatgtc
    14941 gccttcgtta gcaatcgcac caacgatatg acgaacttca acaccatcat cgcggcccac
    15001 ttcaatgcgg tacagctgca tatcgccaac atcacgacgt tcacgacgcg gacgatcttc
    15061 acggtcacca cgcgggccac ggtcgttacg atcgcgcgga ccacggtcat cacggtcacg
    15121 gaattcacgt ttcggacgca tcggcgcatc tggcggtacg atcagagtac gttcaccctg
    15181 tgccattttc agcagtgccg cagccagagt ttcgagatcc agctcttcac cttctgcagt
    15241 cggctgaatt ttgctcagca gtgcgcggta ttgatccaga tcgctgcttt ccagctgctg
    15301 ctgtacttta gcggcgaatt tttccagacg gcgtttgccc agcagttctg cgttcggcag
    15361 ttctacttcc ggaatagtca gcttcatagt acgttcaatg ttgcgcagca gacgacgctc
    15421 gcggttctca acgaacagca gcgcgcggcc agcacgaccc gcacgaccgg tacgaccgat
    15481 acggtgaacg taagactcag aatccatcgg gatatcgtag ttaactacca ggctgatacg
    15541 ctcaacgtcc aggccacggg ctgcaacgtc ggtcgcaatc aggatgtcca gacgaccatc
    15601 tttcaggcgt tccagtgtct gttcacgcag cgcctggttc atgtcaccgt tcagcgcggc
    15661 gctgttgtag ccgttacgct caagagcttc agccacttcc agagtcgcgt ttttggtacg
    15721 aacgaagata atcgccgcat caaaatcttc cgcttccagg aaacgtacca gtgcttcgtt
    15781 tttgcgcata ccccagacag tccagtagct ctggctgatg tcaggacggg tagtcacgct
    15841 ggactgaatg cgcacttcct gcggctcttt cataaagcgg cgggtaatgc gacgaatcgc
    15901 ttccggcatg gttgcagaga acagagcggt ctgatgacct tccgggatct gcgccataat
    15961 ggtttcaacg tcttcgatga agcccatgcg cagcatttcg tcagcttcat ccagaaccag
    16021 accgctcagt ttagagaggt ccagagtgcc acgtttcagg tggtccagca gacggcccgg
    16081 agtaccgaca acgatctgcg gcccctgacg cagggcgcgt aattgcacgt cataacgctg
    16141 gccgccgtac agagcaacca catttacgcc gcgcatgtgt ttagagaaat ccgtcattgc
    16201 ttcagcaacc tgtaccgcca gttcgcgggt cggtgccagc accagaatct gtggtgcttt
    16261 cagctcagga tcaagattct gcaacagagg taaagagaat gctgcagttt ttccgctccc
    16321 cgtctgggcc atacccagaa cgtcgcggcc attcagcaga tgtggaatac actctgcctg
    16381 aattggagat ggtttttcgt aacccagatc gttaagggct tcaaggatag gagccttcag
    16441 gcccagatct gcaaaagtgg tttcgaattc agccatgtag tacgtgtgcc tcaaaattaa
    16501 tggcggccag tctacataac tcatcatgaa attgatcagc aattttcatt gaaaagtgtg
    16561 aaccggctca aagtaggtgt attaacgaac aacaacgccc tcacccgtta aggtgatggc
    16621 aatcaaaaaa gattacgggc tgatgtgtac gtcagctatt gctggtccga ttctgccagg
    16681 tcatcttggt cctggcccag gagcgataat tccaacaatg cgtatcggtg ctcaacaaag
    16741 ttatgaacgt tgttggcaac cgccagtttg aacagtgccg tggcgctgtc caaatccccc
    16801 agacttaggt agtacttacc taaatagaag ttggtttcac tgagatgctc agcgagcgag
    16861 gtgttatccg ttgcgtccgc cttgagcctt tccattaacg tttgttcgct aatgttgccc
    16921 aggtagaact cgacaatgtt ccatccccac tgttccttat ccgatttttc gaagtgctgt
    16981 ttcaacactt ctttagcctg cttctcatcg agcttctgct cggcgagata aagccacaga
    17041 ctacggaaag gatcattggg atcgtcttga taaaacgcca gcagatcatc ttgcgctaac
    17101 ttgtcacgac cgccgtaata taatgcgatc ccgcgattca agtgcgcgta gttgtaagtt
    17161 ggatcaagct caagtacaga atcaaacgct tcataggcag catcaaaatt gcctgcctgc
    17221 gttaaatata tgcctaagta attgaatact tcaggcatat ccggtcggat tgccagcgct
    17281 tgcgaaaaat cgttacgcgc taatgccctc agaccgagac tatcatacaa cactccgcgc
    17341 tcatataaaa gctgtgcgcg ttcgtcatcg gttaaagccc gactggcaag gatttgttcc
    17401 atacgtgcca gaatcacttc ctgctgtaaa gtcggttgca atggtaccgc gaggacttca
    17461 cttttacgcc aggaagtatt actgcatcct gcaagcgtaa gtgctgtcgc aacgaaacac
    17521 cagcgcaaaa aaggcttcat ttcccactcc cgaagaccac ggttgaatga acgtcctgtt
    17581 cccggttgct aacaaggcgt cctgcccggt taaaagcccc ccgccgcagc ggagggcaaa
    17641 tggcaacctt actcgccctg ttcagcagcc ggagcttccg gtgctgcagc aggttgagac
    17701 tgctcagtcg cttctttaat gctcagacgg atacggccct ggcgatcaac ttctagaact
    17761 ttcaccggta cttcctgacc catctgcagg taatcggtca ctttctcaac gcgtttgtca
    17821 gcgatttgag agatgtggac cagaccttct ttaccgccgc cgatggcaac aaatgcgcca
    17881 aagtcaacga tacgggtcac tttaccagtg tagacgcggc ccacttcgat ttctgcagtg
    17941 atctcttcga tacgacgaat agcatgtttc gctttctcgc cgtcggtcgc tgcgatcttc
    18001 acagtaccgt catcttcgat ttcgatggta gtgccagttt cttcggtcag ggcacggata
    18061 acagagccgc ctttaccgat aacgtctttg atcttatccg ggttgatctt gatggtatgg
    18121 atacgcggtg cgaactcaga gatatcgcca cgcggcgcgt tgatcgcctg ttccattacg
    18181 cccaggatat gcagacgcgc acctttagcc tggttcagcg caacctgcat gatctctttg
    18241 gtgatacctt caattttgat atccatctgc agtgcagaga taccgtcgcg ggaacctgca
    18301 actttgaagt ccatatcgcc caggtgatct tcgtcgccca aaatgtcaga cagtacaacg
    18361 tagttgtcgc cttctttcac cagacccatt gcgatacccg caacggcagc tttgattggc
    18421 acacctgcgt ccatcagcgc cagagacgcg ccgcacacgg aagccataga agaagaaccg
    18481 ttggattcgg tgatttcaga cactacacgt acggtgtacg ggaatttgtc catatccggc
    18541 atgactgcca gcacgccgcg cttcgccaga cgaccgtgac caatttcacg acgcttcgga
    18601 gaaccgacca tgccggtttc gcctacggag tacggaggga agttgtagtg gaacaggaag
    18661 gtatcggtac gttcgcccat cagttcatca agaacctgcg cgtcacgagc ggtacccagc
    18721 gttgcagtaa ccagtgcctg cgtttcaccg cgggtgaaca gcgcagaacc gtgagtacgc
    18781 ggcagcacgc cagtacgcac atccagacca cggatcatat ctttttcacg accgtcgata
    18841 cgcggttcgc ctgccagtac gcggctacga acaacgtttt tctcgatagc gtgcagaatt
    18901 tcacccagtt cgttttcgtc cagggtttcg tcttcagcaa gcagcgtcgc gatggtttca
    18961 gatttgatga catcaacctg cgcataacgc tcttgtttgt cggtgatgcg gtaagcatca
    19021 ctcagacgag cttcagccag tgcagcaacg cgcgcgttca gcgcttcgtt taccggctcc
    19081 ggctgccagt cccaacgcgg tttaccggct tctttcacta gttcattgat gttctgaata
    19141 acaacctgct gttgttcatg accgaacact actgcgccca gcatctgatc ttcgctcagc
    19201 agttcagctt cagattcaac catcagtaca gcggcttcag taccggcaac aaccagatcc
    19261 agtttgctct ctttcagctc gtcctgagtc gggttcagta cgtactggtc attgatgtaa
    19321 cctacgcggg cagcaccaat cgggccattg aacggaatac cagacagaga cagtgctgcg
    19381 gaagcaccaa tcatcgcgac gatatccggg ttaacctgcg ggttaacaga aaccacggtg
    19441 gcgataacct gaacttcgtt gacgaagcct tccgggaaca gcgggcgaat cgggcggtca
    19501 atcagacgcg cgatcagggt ttcgccttcg cttggacgac cttcacgacg gaagaagcta
    19561 cccgggatac gaccagcagc gtaggtacgc tcctgatagt taacggtcag tgggaagaag
    19621 tcctgacctg gtttggcttt tttctgtcca acaacggtaa cgaataccgc ggtgtcatcc
    19681 atgctaacca taacagcggc agtagcctga cgagccatca tgccggtttc cagagtcacg
    19741 gtgtgttggc cgtactggaa tttacgaacg atcggattaa gcaatgtaat atcctttctc
    19801 tttcttagac agtaccttac ggcactggtg ttaatacccg atcttctgcg catcctcgcg
    19861 actaatgaca accctaaccc agctctatgt gggtaaagcc tctcattagc cgcgcgaacc
    19921 tctgcaacgg aagatcattc atagcaacaa tacattagtt tccagtgaat tgctgccgtc
    19981 agcttgaaaa aaggggccac tcaggccccc ttttctgaaa ctcgcaagaa ttagcgacgc
    20041 agacccaggc gctcgatgag ctgggtgtaa cgtgctacgt ctttacgttt caggtagtcg
    20101 agcagtttac gacgctgaga aaccatgcgc agcagaccac gacggctgtg gtgatctttt
    20161 ttgtgctctg caaagtggcc ctgcaggtgg ttgatctgtg cagtcagcag tgctacctga
    20221 acttcggtag aaccggtgtc gtttgcgtca cgaccaaact cagaaacgat tttagctgtt
    20281 gcttcagtac ttagagacat tttaaaactc caaagtatat agaatgaaag gacgccgatc
    20341 tctaattcag cgatcccagt gtacgttacg caaagtgtta aacaatttac gcgacgttaa
    20401 gcggcagtat tctactcgta gcgacctgtt atcgcaagac ggttaacatt acgccgggta
    20461 ttcaaccacc aggcgacgag gcgcgacgcg gccttcatcg tcaatttcgc ccataccgat
    20521 aaacttgccg ttctcacctt ctgtaacgcg aaccagtcct tccagcggcg caccggatgt
    20581 acgaactggg ttaccatttt tgaagtaaac agaagacgtt aacggaagat tcaccaccgg
    20641 gtagtccgaa gctggactgt ccattggcat cagtaatgga tcaagtaact ccgcagccgg
    20701 aatatcctgc tgttcggctt gttcgaccag ttcacgcaga tgttccaggg tcaccatccg
    20761 ttcaaccgga tatttactta ccgccagacg acgcaggtaa ataacatgcg cgccacaacc
    20821 gagtttttca cccaggtcat caatgatggt gcggatataa gtgccttttg agcagtgaat
    20881 ttccagctcc agctcattgc cttcgtggcg aataaacagc aattcataaa cggtaatcgg
    20941 gcgcgcttca cgcggtactt caatgccctg acgcgcatat tcgtacagtt ttttaccctg
    21001 atatttcagt gctgaataca tcgaagggat ctgttcgata tcgccacgga aagtatccag
    21061 cgccgccgcc agttgctctg cgctaaaggt taccggacgt tcttcaacga tttgtccgtc
    21121 ggcatcagaa gtatcggtac gctgtccaag acgcgcaatg acccgatagc gtttgtcgga
    21181 gtccagcaga tactgggaaa acttcgtcgc ttccccgagg caaattggca acatgcctgt
    21241 tgccagcggg tccagcgcac cggtatgccc ggcacggttg gcgttataaa tccgtttcac
    21301 tttttgcagc gcatcattgc tggacatacc ctgcggttta tccagcaaca aaacgccgtt
    21361 aatgtcgcga ccgcgacgac gaggacgact cattagtcct ccttgctgtc gtccgggtta
    21421 acacgacgtt cttcgtcatg tttgaccacg ctggtcacca ggtttgacat gcgcatccct
    21481 tcaaccagag agttgtcgta gaagaaggtc agttccggca cgatacgcag gcgcatcgct
    21541 ttccccagca ggctgcggat gaaaccagaa gcttcttgca acgctttgat gcccgcttta
    21601 accgcgtctt catctttgtc gttgaggaac gtcacatata ctttggcata cgccaggtcg
    21661 cgagacattt cgacaccgga aacggtggtc atcatgccca ggcgaggatc tttaatttca
    21721 cgctgcagga tgagagcgat ctctttttgc atttcctgcg ctacgcgctg cgggcgacca
    21781 aattctttcg ccataataaa ttctcctgac aaaaaagggg ctgttagccc ctttttaaaa
    21841 ttaatttcag gtggaagggc tgttcacgtt ggcctgataa gacgcgccag cgtcacatca
    21901 ggcaatccat gccggatgcg gcgtaaacgc cttatcccgc atggaaccct aaaaacctta
    21961 agcgatggta cgttggatct cgatgatttc gaatacttcg atcacatcgc cagtgcggac
    22021 gtcgttgtag ttcttaacgc cgataccaca ttccataccg ttacggactt cgttaacgtc
    22081 atctttgaag cggcgcaggg actccagctc gccttcgtag ataaccacgt tgtcacgcag
    22141 aacgcggatc gggttgtgac gtttaaccac accttcggta accatacagc ctgcgatagc
    22201 accaaatttc ggcgatttga acacgtcacg aacttccgcc agaccgataa tctgctgttt
    22261 cagttccgga gacagcatac cgctcatcgc cgctttcact tcgtcaatca ggttatagat
    22321 gacggagtag taacgcagat ccaggctttc cgcttcaatc actttacgtg cagaggcatc
    22381 agcacgtacg ttgaagccaa ccaggatggc gttggacgcc gcagccaggg tggcgtcggt
    22441 ttcggtgata ccacctacgc cagaaccgat gatcttcact ttaacttcgt cagtagacag
    22501 tttcagcaag gagtcggaga tcgcttccac agaaccctgt acgtctgcct tcaggacgat
    22561 gttcacttcg tgaacttcgc cttcggtcat gttggcgaac atgttctcga gtttagattt
    22621 ctgctgacgc gccagtttaa cttcgcggaa tttaccctga cgatacagtg caacttcacg
    22681 cgctttcttc tcgtcacgta caacggtaac ttcatcaccc gcagccggta cgccggacag
    22741 gccgaggatt tccaccggaa tggacggacc cgcttccagc acttcctgac ccagttcgtt
    22801 acgcatcgca cgaacacgac cgtattcgaa gccacacaga acgatatcgc ccttgtgcag
    22861 agtaccttca cgtaccagaa cggtagcaac cggaccacga cctttatcga ggaaggattc
    22921 gataaccgca ccgctcgcca tacctttacg taccgctttc agctccagaa cttccgcctg
    22981 cagcaggata gcgtccagca gttcatcgat gccggtaccc gctttcgcag atacgtgtac
    23041 gaactggctt tcaccgcccc actcttccgg caggatgcca tactgggaga gttcgttctt
    23101 aacacgatcc ggatcggctt ctggtttatc aatcttgttc actgcaacca ccaccggtac
    23161 ctgcgccgct ttcgcgtgct ggattgcttc gatggtctgc ggcatcacgc cgtcgtcggc
    23221 agcaacaacc aggactacga tgtccgttgc ctgcgcacca cgtgcacgca ttgaagtaaa
    23281 cgcggcgtgc cccggggtgt ccaggaaggt gatcatgccg ttttcagttt caacgtggta
    23341 tgcaccaatg tgctgggtaa tgccgcccgc ttcgccagag gcaactttcg ttgaacgaat
    23401 gtagtccagc agagaggttt taccgtggtc aacgtgaccc atgatggtca caaccggcgc
    23461 gcgcggttca gccgcagcac ccgtgtcacg gtcgctcatt accgcctctt ccagctcgtt
    23521 ttcacgacgc aggataactt tgtggcccat ctcttcagca accagctgtg cggtttcctg
    23581 atcgataacc tggttgatgg ttgccattgc gcccagtttc atcatcgctt tgatgacctg
    23641 agagccttta accgccatct tgttcgccag ttcgccaacg gtgatggttt cgccgatcac
    23701 aacgtcacgg ttaacggcct gagctggctt ctggaagcct tgctgcaggg aagaaccttt
    23761 acgttttccg cctttaccgc cacgtactgc tgcgcgtgct tcttcacgat cagcttttga
    23821 ttcagcgtgt ttgttgcctt tcttcggacg cgctgctttc gcgttacgac cacggccacg
    23881 accgccttcg acttcacgat cgctttcgtc ttctgcctgg cgagcatgtt gagaagtagt
    23941 gacgtgataa tcgctggaat cttcagtcgg ttccgcgtta tcagtccatt tgttttcttc
    24001 cgccatacga cgtgcttctt cagcaacgcg acgtgcttct tcttcgagtt tacgatgcgc
    24061 ttcttcttcg gctttacgct tgagctctgc agcttcctgc tcacggcggg ctttttcagc
    24121 ctgggcgttt ttagtcatat cgtcttgttg attgctcact ttgtcttttt ccgcagcttc
    24181 acgtttcgct tgttcagcag cttcacgctt agcttgttct gcagcctcac gttcagcttt
    24241 ttgttgcgcc tcgcgtttag ccgattcttc tgcctcacga cgggcttgct cttccgcttc
    24301 acgctgcgct tgctcttccg ctgcaaggcg ttcagcctct tgcggatcgc gtttcacaaa
    24361 ggtgcgtttc ttgcggactt cgatttgtac cgatttgctt tttccaccgg taccaggaat
    24421 gttaagggtg ctgcgtgttt tacgttgcag cgtcaatttg tccgggcctg aatttttctg
    24481 attcaggtgg tcaatcaaag tctgtttctc ttgtgcagac acagagtcgt cagcagactt
    24541 ccggatacct gcatcagcaa attgctgtac caggcgttcc acggaggtct gtcgctctgc
    24601 ggccagcgtt ttaatcgtta catctgtcat gctgttcctt cctgctacag tttattacgc
    24661 ttcgtcaccg aaccagcaaa tattacgggc agccataatc agtgctccgg ccttttcgtc
    24721 ggtcaaccct tcgatatcag ccagatcatc aatgccctgt tcggcgagat cttccagcgt
    24781 acaaacgcca cgggcggcca gtttgaatgc caaatcacga tctacccctt caaggttcag
    24841 cagatcgtca gccggtttgt tatcaccgag gctttcttcc tgggcctgtg caatggtggc
    24901 cagtgcattt ttagcacgct cgcgcagtgc ttcaacggtc ggctcatcaa ggccttcgat
    24961 ttccaacagc tctttcatcg gcacataggc cagttcttcc agcgtcgaga agccttcttc
    25021 taccagaaca gtcgcgaagt cttcgtcgat gtcgagatat ttggtgaagg tgtcgatcgc
    25081 tgcgtgcgct tccgcctgat gcttagcctg caggtcgtca acggtcatca cgttgagttc
    25141 ccaaccgctc agctgcgaag ccagacgcac gttctgaccg ttacggccaa tcgcctgcgc
    25201 caggttaccg gcttcaacgg cgatatccat ggtgtgttta tcttcatcca ccacgataga
    25261 agcaacgtct gccggtgcca ttgcgttaat cacgaactgc gccgggttat catcccacag
    25321 gacgatatcg atacgctcgc cacccagttc agtagacacc gcctgaacac gcgcgccacg
    25381 catacctacg caagcaccta ccggatcgat acgtttatcg ttggttttca ccgcgatttt
    25441 cgcacgagaa cccggatcgc gagccgctgc tttaatttca atcacttctt cgccgatttc
    25501 tggcacttca atacggaaca gttcgatcag catttccggc ttggaacgag tgacgaacag
    25561 ttgcgcgcca cgcgcttccg ggcgaacgga ataaagcacg ccacgaacgc ggtcgccagg
    25621 gcggaagttt tcacgcggca gcatatcttc acgcaggatc acggcttcag cgttgttgcc
    25681 cagatccaga gagatgttgt cgcggtttac ttttttcacc acgccggtga tgatttcacc
    25741 ttcgtgttca cggaactgat caaccaccat cgcacgttcg gcttcacgca ctttctgcac
    25801 gataacctgt tttgccgtct gggtagtgat acggtcaaag gtaacagact caatctgatc
    25861 ttcaacgtaa tcgcccaggt tcaggctttc atcttcataa cgtgcggctt caagggtgat
    25921 ttccttggtc ggctgggtga cttcatcaac aactaaccag cgacggaagg tgtcaaaatc
    25981 accgcttttg cgatcgatct gtacgcggac gtcgatctct tgttcatatt ttttctttgt
    26041 tgctgtcgcc agcgcgcttt ccaatgcttc gaaaatcttc tcgcgaggta gcgccttttc
    26101 attggatacg gcttcaacta cagccaaaat ttctttgttc atcgcgggct tttcacctca
    26161 tccagactat taaaagtggg gaaccaggtt cgccttctgg atattactca gcgcgaacac
    26221 ttcatcttta ccttcgacgg taactgtgat catttcaccg tctaccgctt tgataacgcc
    26281 ctgccattta cgacggtttt gtaccgccat acggagaacc agagtcacct cttctccgac
    26341 aaaacgggcg tagtgttcag ccgtgaacag tgggcgatcg agacccggtg aggagacttc
    26401 caggttataa gcaacggtga tgggatcttc aacatccagc acagcactta cctggtggct
    26461 cacatcagca caatcatcaa cattgatgcc atcttcacta tcaatataga tgcgcagtgt
    26521 ggatgtgcga ccgcgaataa attcgatgcc aaccagttca aaacccaggg cctcaactgg
    26581 cgcagtaatc atctctgtta atttttgctc taatgtggac aagcccaccc ccaagacata
    26641 aaaaaagggc ctaaagccca gttattctgt agtcagataa caaaaaaccc cgataaatcg
    26701 gggctttata taactgaacc ctataaccgc aactgcggtc tggagcactt tccagaagga
    26761 ttttttcaaa tcccactacg aaggccgaag tcttcacagt atatttgaaa aaggactcta
    26821 agggaaagtg gttgcggggg ccggatttga accgacgacc ttcgggttat gagcccgacg
    26881 agctaccagg ctgctccacc ccgcgcctga aacgtggcaa attctactcg ttttgggtaa
    26941 aaaatgcaaa tactgctggg atttggtgta ccgagacggg acgtaaaatc tgcaggcatt
    27001 atagtgatcc acgccacatt ttgtcaacgt ttattgctaa tcatgtgaat gaatatccag
    27061 ttcactttca tttgttgaat acttttgcct tctcctgctc cccct
//
LOCUS       DADMKF010000046        26864 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.047, whole genome shotgun sequence.
ACCESSION   DADMKF010000046 DADMKF010000000
VERSION     DADMKF010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26864
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.047"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            202..474
                     /locus_tag="JNC24_001755"
     CDS             202..474
                     /locus_tag="JNC24_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid shock protein"
                     /protein_id="HAY9158512.1"
                     /translation="MGLSSAAFAAETATTPAPTATTTKAAPAKTTHHKKQHKAAPAQK
                     AQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHSHQQPAKPAAQPAA"
     gene            567..650
                     /locus_tag="JNC24_001756"
     CDS             567..650
                     /locus_tag="JNC24_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9158513.1"
                     /translation="MLGRYRFEFILIILILCALIAARFYLS"
     gene            750..1571
                     /locus_tag="JNC24_001757"
     CDS             750..1571
                     /locus_tag="JNC24_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HAY9158514.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTNDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(1610..1939)
                     /gene="mdtI"
                     /locus_tag="JNC24_001758"
     CDS             complement(1610..1939)
                     /gene="mdtI"
                     /locus_tag="JNC24_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HAY9158515.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(1926..2291)
                     /gene="mdtJ"
                     /locus_tag="JNC24_001759"
     CDS             complement(1926..2291)
                     /gene="mdtJ"
                     /locus_tag="JNC24_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HAY9158516.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            2703..3737
                     /gene="tqsA"
                     /locus_tag="JNC24_001760"
     CDS             2703..3737
                     /gene="tqsA"
                     /locus_tag="JNC24_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HAY9158517.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLISVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            complement(3762..5150)
                     /gene="pntB"
                     /locus_tag="JNC24_001761"
     CDS             complement(3762..5150)
                     /gene="pntB"
                     /locus_tag="JNC24_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HAY9158518.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(5161..6693)
                     /gene="pntA"
                     /locus_tag="JNC24_001762"
     CDS             complement(5161..6693)
                     /gene="pntA"
                     /locus_tag="JNC24_001762"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HAY9158519.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYTVPGEIFTTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLASVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            7217..8161
                     /locus_tag="JNC24_001763"
     CDS             7217..8161
                     /locus_tag="JNC24_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HAY9158520.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEILGVATTA
                     SPSSEVGRFFETQSSKGGRYTVMLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQERGNNLTVSADLYK"
     gene            8347..9729
                     /locus_tag="JNC24_001764"
     CDS             8347..9729
                     /locus_tag="JNC24_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAY9158521.1"
                     /translation="MKKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            9766..10488
                     /gene="folM"
                     /locus_tag="JNC24_001765"
     CDS             9766..10488
                     /gene="folM"
                     /locus_tag="JNC24_001765"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HAY9158522.1"
                     /translation="MGKAQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLIKAGAQCIQADFSTNDGVMAFADEVLKSPHGLRAILHNASAWMAEKPGAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHIAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(10485..10820)
                     /locus_tag="JNC24_001766"
     CDS             complement(10485..10820)
                     /locus_tag="JNC24_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HAY9158523.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVSCWGISAWV
                     LIICWIKLH"
     gene            10949..11668
                     /gene="rstA"
                     /locus_tag="JNC24_001767"
     CDS             10949..11668
                     /gene="rstA"
                     /locus_tag="JNC24_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HAY9158524.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWE"
     gene            11672..12973
                     /gene="rstB"
                     /locus_tag="JNC24_001768"
     CDS             11672..12973
                     /gene="rstB"
                     /locus_tag="JNC24_001768"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HAY9158525.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALITFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLAQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDTSELGGARFSFSWPLWHNIPQFTSA"
     gene            13049..13978
                     /gene="tus"
                     /locus_tag="JNC24_001769"
     CDS             13049..13978
                     /gene="tus"
                     /locus_tag="JNC24_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HAY9158526.1"
                     /translation="MARYDLVDRLNTTFRQMEQELAAFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(13975..15378)
                     /gene="fumC"
                     /locus_tag="JNC24_001770"
     CDS             complement(13975..15378)
                     /gene="fumC"
                     /locus_tag="JNC24_001770"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HAY9158527.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLGLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQVKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
     gene            complement(15521..17167)
                     /locus_tag="JNC24_001771"
     CDS             complement(15521..17167)
                     /locus_tag="JNC24_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HAY9158528.1"
                     /translation="MSNKPFHYQAPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGCDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            17366..18541
                     /gene="manA"
                     /locus_tag="JNC24_001772"
     CDS             17366..18541
                     /gene="manA"
                     /locus_tag="JNC24_001772"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416130.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HAY9158529.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     RNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPNAERLSELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            18642..20150
                     /locus_tag="JNC24_001773"
     CDS             18642..20150
                     /locus_tag="JNC24_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HAY9158530.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLK
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLASFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATEFMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYTNGQITLNGQKMPLEDFVGMFAMPALNVPAVPAIPQQ"
     gene            complement(20331..21641)
                     /gene="uidC"
                     /locus_tag="JNC24_001774"
     CDS             complement(20331..21641)
                     /gene="uidC"
                     /locus_tag="JNC24_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HAY9158531.1"
                     /translation="MRKIVAMAVICLTAASGLISAYAAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQGGLLDFNSGYYSNIIGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSENSLLKSGRFGTDYSYGSLPYRIPLMAGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWHDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGVN
                     YRLTPQFTLTFAQIVTRGYESSKRDVRIEGAGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSTLGDRLNYWDVKMSIQYDFMLKSEITPERKTSGVSDC"
     gene            complement(21680..23053)
                     /gene="uidB"
                     /locus_tag="JNC24_001775"
     CDS             complement(21680..23053)
                     /gene="uidB"
                     /locus_tag="JNC24_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
                     /protein_id="HAY9158532.1"
                     /translation="MNQQLSWRTIVGYSLGDVANNFAFAMGVLFLLSYYTDVAGVGAA
                     AAGTMLLLVRVFDAFADVFAGRVVDSVNTRWGKFRPFLLFGTAPLMIFSVLVFWVPTD
                     WSHSRKVVYAYLTYMGLGLCYSLVNIPYGSLATAMTQQPQSRARLGAARGIAASLTFV
                     CLAFLIGPSIKNSSPEEMVSVYHFWTIVLAIAGMVLYFICFKSTRENVVRIVAQPSLK
                     ISLQTLKRNRPLFMLCIGALCVLISTFAVSASSLFYVRYVLNDTGLFTVLVLVQNLVG
                     TVASAPLVPGMVARIGKKNTFLIGALLGTCGYLLFFWVSVWSLPVALVALAIASIGQG
                     VTMTVMWALEADTVEYGEYLTGVRIEGLTYSLFSFTRKCGQAIGGSIPAFILGLSGYI
                     ANQVQTPEVIMGIRTSIALVPCGFMLLAFVIIWFYPLTDKKFKEIVVEIDNRKKVQQQ
                     LISDITN"
     gene            complement(23050..24861)
                     /gene="uidA"
                     /locus_tag="JNC24_001776"
     CDS             complement(23050..24861)
                     /gene="uidA"
                     /locus_tag="JNC24_001776"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HAY9158533.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRA
                     IAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     KKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     gene            complement(25250..25840)
                     /locus_tag="JNC24_001777"
     CDS             complement(25250..25840)
                     /locus_tag="JNC24_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAY9158534.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIKGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTSMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            complement(26061..26828)
                     /gene="hdhA"
                     /locus_tag="JNC24_001778"
     CDS             complement(26061..26828)
                     /gene="hdhA"
                     /locus_tag="JNC24_001778"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005153726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HAY9158535.1"
                     /translation="MRHAPCAMRHAPCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLGGQAFACRCDITSEQELSALADFAISKLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNGIAPGAILTDALKSVITPEIEQKMLQHTPIRRL
                     GQPQDIANAALFLCSPAASWVSGQILTVSGGGVQELN"
BASE COUNT         6740 a         6957 c         6463 g         6704 t
ORIGIN      
        1 ggttatcgct cgccacggga atatctgcgg cagcgggctt gtaatgggtt aagtgataac
       61 agatgtctgg aaatataggg gcaaatccac tgccattccc agggatatag ttatttcaac
      121 ggccccgcgg tggggttaaa tgaaaaaaca aattgagggt atgacaatga aaaagtatta
      181 gctctggttg ttgccgctgc tatgggtctg tcttctgccg cctttgctgc agagactgcg
      241 accacacctg ctccgactgc gacgaccacc aaagcagcgc cggcgaaaac gacacatcat
      301 aaaaaacagc ataaagcagc acctgcccag aaagcgcagg cggctaaaaa gcatcataaa
      361 aatacgaaag ctgaacagaa agcccctgaa caaaaagcgc aggcagcgaa gaaacacgcc
      421 gggaaacaca gccatcagca accggcaaaa cctgctgcac aacccgcagc gtaagttttc
      481 aacagtaatg ctggcgcgcc ccctcgcgcc tgaaaattac ggtgctaagc gggtaacgtt
      541 tagcaccgcc tttagcggag ggtagtatgc tgggccgtta tcgctttgag ttcattctta
      601 tcatccttat tttatgcgca ctgattgccg cccgttttta tctttcctga ctgtagttat
      661 ctgattttac tcccactttc atcccgtccc gtctatagta tttatgaggg tttgctttta
      721 ataatcataa ttacccacca gagtgtgata tgcgtacaac cattgctgta gtgttgggtg
      781 caattagttt gacgtctgct tttgtgtttg cagataaacc agacgttgcc aaatcggcaa
      841 acgatgaggt cagcaccctg ttttttggtc atgatgatcg tgtgccagtg aatgacacga
      901 cccaatcacc gtgggatgcg gttgggcaac tggaaacggc cagcggcaat ttatgtacgg
      961 cgacgctgat tgcacccaat ctggcattaa cggcaggaca ctgtttattg acacctccaa
     1021 agggtaaagc cgataaagca gtggcgctgc gttttgtgtc aaataaaggt ctttggcgct
     1081 atgagatcca cgacatagaa ggccgcgttg atccgacact gggaaagcgg ttaaaagcag
     1141 atggggatgg ttggattgta cctcccgcag ccgcgccgtg ggacttcgga ttgattgtgc
     1201 tacgtaatcc cccttctggc attacaccgt tgccgttatt tgagggagat aaagccgcgc
     1261 ttactgccgc attaaaatcg gctggccgta aagtaactca ggcgggatac cctgaagatc
     1321 atctcgatac gttgtacagt catcaaaact gtgaagtgac tggctgggcg caaacgtcgg
     1381 tgatgtcaca tcagtgcgat accttgccgg gtgacagcgg ttcgcctctg atgttgcata
     1441 ccaatgacgg ctggcaatta attggggtgc aaagttcggc tcctgccgcg aaagatcgct
     1501 ggcgcgccga taaccgggcc atttctgtta ccggttttcg cgacaagctg gatcaactgt
     1561 cgcaaaaata atgttcaaac gctgcccgac tgtgcgggca gcgtgttcat caggcaagtt
     1621 tcaccatgat cattccagcc aacagcaaga ccaggccaat ccagccttta cgatttaacc
     1681 gttgaccaaa caagatccaa cctgcggcta acgtggcggc aataccaaac ccgccccaca
     1741 atgcataagc gacagacaag tcgatccctt taacggcctg agaaagcgca ctaaaggcag
     1801 ccagcaccgc cgccagggag agcaagccaa atattttgcg acgaaagccg tcagaaaatt
     1861 tcaaaaagac gttagcaacg atttccagca cgattgccaa tgccagccag gcggcgtgaa
     1921 cccattcaaa ctgcgccatg gttcacctcc agttcaggtt tacgcgcttt acgggtacct
     1981 gattttatca acacaatccc ggcgaccagg gtggttaacc cggcaatttt catcagcgat
     2041 aaactttcgt cgaataacaa aacgctaaac aaggtaataa ataaaatacc gataccttcc
     2101 cacagcgcat aagctacgcc taaggcgatc tttttaacgg cgaaagagag aaatatatac
     2161 gacagagaaa tcatcaccag cattaaaata aagccgccat ttccctcact gacgctcgcc
     2221 catttcattg acagcgtacc ggtaatttct gtagcaatag ccaggcctaa taaaatccaa
     2281 taaatataca ttgtccttct cctgcaagag aattatttta attttcgctt aattcagcga
     2341 aaccaaagta aagtggtcaa gccccgcgca tagggcaagc ttaggcagaa gaaaggacta
     2401 aagcgcgttg cgccagtgct gctcacctac gagcaagata gtggatgagg tacgaagatg
     2461 ggtaaatgtt gaaaataacg tcctgaacaa attgtccata atattacaat tatccgcagt
     2521 gttgcttctc gtcatcgcgg atgataattg tcctcggtag ttgaacacgc ctgatttgta
     2581 tcatagctta agaattaact caaaatattt tcacttcttt acctgagcgg tttgattttc
     2641 gttatgatga cggagcgaaa aagacattat tattagcaaa ggaagaaaaa acggggacaa
     2701 gcatggcaaa gccgatcatc acgctcaatg gcctaaaaat cgtcattatg ttgggaatgc
     2761 tggtcattat tctctgcggt atccgttttg ccgccgagat catcgtgccg tttattctcg
     2821 cattatttat tgctgttatt cttaacccgc tggtgcaaca catggtccgc tggcgagtgc
     2881 cgcgtgtact ggcggtgtcg attttgatga ccatcatcgt gatggcgatg gtgttgctat
     2941 tagcttatct gggttccgcg ctcaacgagt tgacgcggac gttaccgcaa tatcgcaact
     3001 ctattatgac gccgctgcaa gctcttgagc cgttgttgca acgcgtaggg attgacgtct
     3061 cagttgacca gctggcgcat tacattgatc cgaacgcggc gatgacgttg ctcaccaact
     3121 tattgacgca gttatctaat gccatgtcat caatattttt attgctgctg acggtgctgt
     3181 ttatgctgct cgaagtgcca caattgcccg gaaaatttca gcaaatgatg gcgcgtccgg
     3241 ttgaagggat ggcggcgatt caacgtgcaa ttgacagtgt ttctcattat ctggtgctga
     3301 aaacagccat cagcatcatc accggcctgg tcgcctgggc gatgctcgcc gcactcgatg
     3361 ttcgcttcgc ttttgtctgg ggattgctgg cctttgcgct taattacatc cctaatattg
     3421 gttcagttct cgcggcaatc ccccctatcg ctcaggtact ggtgtttaat ggcttctacg
     3481 aagcgttgct ggtgctggcg ggatatctgc tgattaatct ggtcttcggc aatattctgg
     3541 agccgcgcat catgggacgt gggctggggc tttccacatt ggtggtattt ttgtcgttga
     3601 ttttttgggg atggttgtta ggaccggtgg gtatgctgat ttccgtgccg ttaacaatta
     3661 ttgtcaaaat tgcgcttgaa caaacagcgg gaggtcaaag catcgccgtt ctgttaagcg
     3721 atctcaataa agagtgacgg cctccgcaga ggccgtcagg gttacagagc tttcaggatt
     3781 gcatccacgc tagctttggc gtcaccaaac agcatgtggg tgttttcctt gaagaacagc
     3841 gggttttgca caccagcata gccagtgttc atcgaacgtt taaagacaat cacgttctgc
     3901 gctttccaca cttccagcac aggcatacca gcaataggac tcttcggatc atcctgcgcc
     3961 gccgggttaa ccgtatcgtt agcaccaatc accagtacgg tatcggtatc agcaaagtca
     4021 tcgttgatct cgtccatttc cagcacgatg tcatacggta cttttgcttc agccagcaat
     4081 acgttcatat gtccaggcaa acgccccgca accgggtgga taccgaaacg cacgttgata
     4141 ccacgagcgc gcagtttctc ggtaatttca gcgacaggat attgcgcctg cgcgactgcc
     4201 atgccgtacc ccggagtaat gatcactgaa tgggagtttt tcagcagttc cgctgtctct
     4261 tctgcggtga tttcgcggtg ctcgcccact tcctgatcat cgccagtaga agagccgtcg
     4321 gtgccgaaac cacccgcaat aacgctgata aaggaacggt tcatcgcctt acacataatg
     4381 taagaaagga tagcccccga agaaccgacc agcgcaccgg tcacaatcag caggtcgttg
     4441 ctgagcataa agcccgcagc cgcagccgcc cagccggagt acgagttcag catcgacacc
     4501 accactggca tatctgcacc accgatggag gcgactaaat gccagccgaa taccagcgca
     4561 atggcggtca ttattagcaa tgccagcact tgcaggccga cgctgtccgt gcgaacaaat
     4621 acaatcagca gcaggaagga aacgaccaga gccgccaggt tcattttgtg acggtttggc
     4681 agcatcaacg gtttagacga aatcttgcca cacagtttgc cgaacgccac caccgaaccc
     4741 gtgaacgtta ccgccccgat gaagataccg aggaacactt ccgtcaggtg aatattgacc
     4801 agaatcggtg ccattcccgc gtcatgatgc agatagctgt taaagccaac cagcactgcc
     4861 gccagaccca cgaagctgtg caggatcgcc accagttctg gcatttcggt catttcaact
     4921 ttcttcgcca gacggatacc aattgcccca ccaatgacca tcgccagcaa gatccagcca
     4981 acattacccg tatccggtcc aaaaatggtt gcgattaacg caatcgccat cccggcgata
     5041 ccgaagttgt taccctggcg agacgtttca tgtttcgaaa gaccggccag actgaagata
     5101 aacaggatcg cggcaacaat gtatgcagct gtaactaatc ctccagacat atgttacccc
     5161 ttaatttttg cggaacattt tcagcatgcg ctgagtcacg gtgaagccac cgaaaatatt
     5221 aatgctggct ataagcaccg cgataaaact aaggaagcta acccagccgc cctggccaat
     5281 ctgcaacagt gctccgacaa caataatccc tgaaatcgcg ttggtgaccg acatcaacgg
     5341 tgtatgcagc gcgtgcgata cattccacac cacgtaataa ccgacaacgc aggccagcgc
     5401 gaaaacggtg aagtgcccaa gaaactcttt cggcgcaacg cttgccagcc aaccgaaaag
     5461 gatgattgcc agcgccatca acgcgtattt acgccacggt gagcaggcac atttttcctc
     5521 agttttcact tccggtgccg ctttttgtgc cgcctgcggc tgagctgata cctgaatcgg
     5581 cggtgccggc caggtaattt cgcccgcacg gatcacggtc acgccgcgaa tcaccacatc
     5641 atcaaaatca acagtgatat tgccgtcttt ctctttgcac aacagtttca gcagattaac
     5701 gaggttagta ccgtaaagct gcgaggattg cgtcggcaga cggcccggaa gatcggtata
     5761 accaatcact ttgacaccat tttccgtagt gaagatttca cccggcacgg tgtattcaca
     5821 gttgccaccg ttttgggctg ccaggtcgac aatcacactg cccgccttca tggagtcaac
     5881 catttcacgg gtaattagct tcggcgctgg tttgcctgga ataagcgcgg tggtgacaat
     5941 gatatcgacc tcttttgcct gggcggcaaa gagttccatt tccgctttaa tgaacgcgtc
     6001 cgacatcact ttggcatagc catcgccact gcccgcttcc tctttaaaat ccagctcgag
     6061 gaattccgcg cccatacttt gaacttgttc tttcacttcc ggacgggtgt cgaatgcacg
     6121 cacaatcgcg ccgagactgt ttgctgcgcc aatggcggcc agacctgcaa cacccgcacc
     6181 aatcaccatc acttttgccg gtggcacttt cccggccgca gtaatttgcc cggtaaagaa
     6241 gcgcccaaat tcatgtgccg cttcaacaat ggcgcgataa ccggcgatgt tcgccatcga
     6301 gcttagtgcg tccagcgatt gtgcgcgtga gatacgcggc acagaatcca tcgccatcac
     6361 ggtcacgtta cgttccgcaa gtttttgcat taattccgga ttctgcgcag gccagataaa
     6421 actcaccagc gttgtcccag gattcagtaa cgcaatttca tcatctaacg gcgcattgac
     6481 cttcagaatg atctctgact gccagacgct attcccttct acaatttcag cgcccgcttg
     6541 cacaaacgct ttatcgtcaa aacttgccag ttgacccgcg ccgctctcta ccgcgacggt
     6601 aaaacccagt ttcagcagct gttccactgt ttttggcgtt gctgcaacac gggtttcatt
     6661 ggttaaccgt tctcttggta tgccaattcg catgatattc ccttccatcg gttttattga
     6721 tgatggtttg cctgtgtcag gagccacaca agccgctcat gtacgagcta aatgttactc
     6781 cgttaaaata aattagtaac aaacgcccta taacgtactg aaaattatgc ctgtgatcta
     6841 gcgccaaaaa tcagtatttc ggcgtgaact cgcaaaatat taacgattca gccgtgatag
     6901 tgggataaac accttagaac gccggataaa gactgataat tgtcttcgac ggccgggtaa
     6961 aacgagacat cgccccggca cgattcacta cttaacatta aattaactta tacaattcag
     7021 ttgcttcagt agtaatgatg ctgatacggc tgttttttaa gcatagacgg tcatttgagc
     7081 aggattaaaa ttggcttaag gaatgtgata tgaaaaatga cgcagacagt tacaccgttt
     7141 aaatgcaata atcagccacg tttctcgtta ataacaatac cagtacctgg tttgcgcaag
     7201 gcgaaggatt atttttatga agcttaagaa caccctcctg gcgtcggcac tgctttctgc
     7261 tacggcattc tccgttaacg cagcaacaga actgacaccg gagcaagcgg cagcggttaa
     7321 accttttgac cgtgtagtgg ttaccggtcg ttttaatgct attggcgaag cggtgaaagc
     7381 cgtttctcgt cgcgcagata aagaaggtgc cgcctctttt tatgttgtcg acacttctga
     7441 ttttggtaac agcggtaact ggcgtgtggt cgctgacctc tataaagccg atgctgaaaa
     7501 agcagaagaa acaagtaatc gcgtaattaa cggtgttgtc gaactgccga aagatcaggc
     7561 tgttctgatt gaaccgtttg acacggtcac cgtccagggc ttctatcgta gccagccaga
     7621 agtcaatgat gccatcacca aagcggcaaa agcgaaaggt gcctactctt tctacatcgt
     7681 tcgtcaaatc gatgccaacc aaggcggcaa ccagcgtatt actgcattca tctataaaaa
     7741 agatgctaag aaacgtatcg tccagagccc ggatgtgatc ccggcagatt ccgaagcagg
     7801 acgtgcagct ctggctgccg gtggcgaagc cgcgaagaaa gttgagatcc taggtgttgc
     7861 gactaccgca tcaccaagtt ctgaagtcgg tcgtttcttt gaaacccagt catcaaaagg
     7921 cgggcgttac accgtcatgc tcccggatgg cactaaagtc gaagaactga acaaagcgac
     7981 cgcagcgatg atggtcccgt tcgacagcat caaattctct ggcaactacg gcaacatgac
     8041 cgaagtctcc tatcaggttg cgaaacgtgc cgcgaagaaa ggtgctaagt attaccacat
     8101 cacccgccag tggcaggaac gtggtaataa cctgaccgtc agcgcagatc tgtataaata
     8161 actattcagt tactcctata aggcggcttg atgccgcctt tcgcattttt agtcacaaat
     8221 ttccccgcga ccattgcatg cagacacatc tcttcgtaaa atcccgcgct tcagtggcaa
     8281 actccatttt tatgcgtttt gccctaatat ttattcttct tatcacgttt taatcactgg
     8341 atatcgatga aaaagaaact gggactgagc gcactcaccg cgctggtatt aagctcaatg
     8401 ctgggcgcgg gtgttttcag tctgccgcaa aatatggcgg cagttgccag cccggcagca
     8461 ctgctcatcg gctggggtat tactggcgct ggcattttat tgctggcctt tgccatgctg
     8521 atcctcacgc gcattcgtcc cgaacttgac ggcggtatct ttacctatgc ccgcgaaggg
     8581 tttggcgagc taatcggctt ttgttccgca tggggatact ggctgtgcgc agtcatcgcc
     8641 aacgtctcct atctggtgat cgttttttcc gcgttaagct tttttacgga cacgccggaa
     8701 ttgcgcctgt tcggcgatgg taatacctgg caatcgatcg tcggtgcatc ggctttattg
     8761 tggattgttc actttttgat cctgcgcggg gtgcaaaccg ctgccagcat taacctggtg
     8821 gcgacactgg caaaattgtt gccgctgggt ctgtttgttg tgctggcgat gatgatgttc
     8881 aaactggata ccttcaagct cgacttcacc ggacttgcac ttggcgtacc cgtttgggaa
     8941 caggtgaaaa acaccatgct gatcaccctg tgggttttca ttggtgtgga aggtgccgtg
     9001 gttgtttctg cgcgtgcgcg taataaacgt gatgttggca aagcgacact gctggcggtt
     9061 ctctccgctc tgggcgttta cttgctggta acgctgcttt cgctgggtgt ggtggcacgt
     9121 cctgaactgg ctgaaattcg taacccgtca atggccggtc tgatggtgga aatgatggga
     9181 ccatggggcg aaatcatcat cgctgccggt ttgatcgttt ccgtttgcgg cgcgtactta
     9241 agctggacca tcatggcggc agaagttccg ttcctggctg ccactcataa agcattccca
     9301 cgcatttttg cgcgccagaa cgcacaagct gcgccatccg cctcactgtg gttgaccaac
     9361 atctgcgtgc aaatttgtct ggtactcatc tggctgaccg gttccgatta caacacgttg
     9421 ctgaccatcg cctcagaaat gattctggtg ccctatttcc tggtcggcgc gttcttgctg
     9481 aaaatcgcaa cccgtccact gcataaagcg gtaggtgtcg gtgcctgcat ttatggctta
     9541 tggttattgt acgcctctgg tccaatgcac ttgctgttat ccgttgttct gtatgcgccg
     9601 gggctgctgg ttttcctgta tgcgcgcaaa acgcatacac atgataatgt actgaaccgt
     9661 caggaaatgg tgctgattgg catgctgtta attgcctcag ttccggcgac ctggatgctg
     9721 gtgggataac gttcatccca tcgttggata gcaaaggaga taacgatggg taaagcccag
     9781 cccttgccaa tattaattac tggcggaggt cgtcgcatcg gcctcgccct cgcctggcat
     9841 ttcattaatc aaaagcaacc ggtgattgtc agctatcgga cgcactatcc agccattgat
     9901 ggactgatta aggcaggtgc gcagtgtatt caggctgatt tttcgaccaa cgacggtgtg
     9961 atggcgtttg ccgatgaagt actaaaaagc ccccatggtc tgcgtgctat tttgcataac
    10021 gccagtgcgt ggatggcgga aaaaccgggt gcgccactgg ccgacgtact ggcttgcatg
    10081 atgcagatcc acgttaatac cccatacctg ctcaaccatg cgctggaaag attactgcgt
    10141 gggcacggac acgccgccag cgatatcatt cactttaccg attatgtggt ggagcgcggt
    10201 agcgacaagc atattgcgta tgctgcaagc aaagcggcac tggataatat gacccgctcg
    10261 tttgcccgca agctggcacc ggaagtgaaa gtgaactcta ttgctccatc gctgatcctg
    10321 tttaatgaac atgatgatgc cgaatatcga caacaggcgc tgaataaatc actgatgaaa
    10381 accgcgcctg gcgagaaaga agtgatcgac ctggtcgatt acttacttac cagttgcttt
    10441 gtcaccggac gcagtttccc acttgatggc ggtcgtcatc tgcgttaatg cagctttatc
    10501 cagcatataa tcaacaccca tgcgcttatc ccccaacacg aaacagaacc aacaaaagcg
    10561 gcgggcaagc gcatcattcc ggtgaaatac cacagcgaca ccaggtaaac aaaataggga
    10621 ataatcgacc acatactaaa aatgatggtt gcgcgtaagg cttcaatgcc gcgttcgctg
    10681 gcaacaatat aatgcgcgat aagcgcaaag gtcgggaaaa gtggaatcag cccggcgata
    10741 taataatttt tcgtttttgc taaaacacca atcaacagca ctaccagcgc accgagcgcg
    10801 gctttgatta ccagccccat ttttttacct taacacttcc ataacaagtc atcagtagaa
    10861 tacctgatga aaacttgttt agaaacgatt gatagtaagt aaaaacagcg cggtgtattg
    10921 tgacgttttt atatctaccg tgaatgttat gaacactatc gtatttgtgg aagatgatgc
    10981 ggaagtcggt tcactgattg ccgcgtacct ggcaaaacat gatatgcagg ttaccgtaga
    11041 gccgcgcggc gaccaggccg aagaaaccat tttgcgagaa aatccggatt tggtgttact
    11101 cgacatcatg ctaccaggca aggacggcat gaccatttgt cgtgatttac gcgcaaagtg
    11161 gtctggaccg attgttcttc taacctctct cgatagcgat atgaaccaca tcctggcact
    11221 ggaaatgggt gcctgcgact atattctcaa aacgacgccc cctgctgttt tgctagcgcg
    11281 tttacgtttg catttgcgtc agaatgagca agccacactg accaaaggtc ttcaggaaac
    11341 gtctctgact ccctacaaag ccctgcattt cggcacgttg accatcgatc ccatcaaccg
    11401 cgtagtcacc ctggctaaca ctgaaatctc gctctcgaca gctgatttcg aattattgtg
    11461 ggaattagct acccatgccg ggcaaatcat ggaccgcgat gcattgctga aaaatttacg
    11521 cggcgtcagt tatgacggac tggatcgtag cgtggacgtg gctatttcgc ggttaagaaa
    11581 aaaactgctc gataacgccg cagaacctta tcgcattaaa actgtgcgta acaaaggcta
    11641 tctttttgcg cctcatgcat gggaataagc gatgaaaaaa ctgtttatcc agttttacct
    11701 gttattgttt gtctgcttcc ttgtgatgtc tctgctggtt gggctggtgt acaaatttac
    11761 cgccgaacgc gcgggcaaac agtcgctgga tgatttgatg aacagttcac tgtatctgat
    11821 gcgcagcgaa ttgcgtgaga tccccccaca cgactggggt aagacgctga aagagatgga
    11881 tttaaatctc tctttcgatc tgcgtgtcga gccactgagt aaataccatc ttgatgatat
    11941 ttccatgcac cgactgcgtg gcggcgaaat tgtcgccctg gacgatcagt acacgttttt
    12001 gcagcgtatc ccgcgcagcc actacgtgct ggcagttggt cctgttcctt atctttatta
    12061 tctccatcag atgcgattgc tggatatcgc cctgatcact tttattgcta tttccctcgc
    12121 ctttccggtg tttatctgga tgcgtccgca ctggcaggat atgttaaaac tggaagcagc
    12181 ggcgcaacga tttggcgatg ggcatctcaa tgaacgtatc cactttgatg agggttcgag
    12241 ctttgaacga cttggtatcg catttaacca gatggcggac aatatcaacg ccttgattgc
    12301 cagcaaaaaa cagcttattg acggtatcgc tcacgaactg cgaacaccgt tagtgcgcct
    12361 gcgttatcga ctggagatga gcgataacct gagcgccgcc gaatcccagg cgttgaatcg
    12421 tgatatcagt caacttgaag ctttaattga agagctgctg acttatgccc gactcgatcg
    12481 cccacaaaac gagcttcatc ttagcgaacc agacctgccg ttgtggctgt caacgcatct
    12541 ggcagacatt caggcagtaa cgcccgataa aacggtacgg attaaaacgc tcgcgcaagg
    12601 ccattatgcg gcgttggata tgcgcttaat ggagcgcgtg ctggataatt tgctcaataa
    12661 cgccctgcgg tactgccatt caacggttga aaccagcctg ctactgtcgg ggaatagagc
    12721 gacattaatt gttgaggatg atggcccagg gattgcccca gaaaaccgcg aacatatctt
    12781 tgaacctttt gttcgcctcg atcccagccg ggatcgctca accggcggct gcgggctggg
    12841 gctggcaatt gtccactcta tagcactggc aatgggcggt acggttaatt gtgacaccag
    12901 cgaactgggt ggtgctcgct tctcgtttag ctggccgtta tggcataaca tcccgcaatt
    12961 tacctctgcc tgacactacg cgcacgatgg tcaagtcacc accactgtgc tataaaataa
    13021 gtatgttgta actaaagtgg ttaatattat ggcgcgttac gatctcgtag accgactcaa
    13081 cactaccttt cgccagatgg aacaagagct ggctgcattt gccgctcatc ttgagcaaca
    13141 caagctattg gttgcccgcg tgttctcttt gccggaggta aaaaaagagg atgagcataa
    13201 tccgcttaat cgtattgagg taaaacaaca tctcggcaac gacgcgcagt cgctggcgtt
    13261 gcgtcatttc cgccatttat ttattcaaca acagtccgaa aatcgcagca gcaaggccgc
    13321 tgtccgtctg cctggcgtgt tgtgttacca ggtcgataac ctttcgcaag cagcgttggt
    13381 cagtcatatt cagcacatca ataaactcaa gaccacgttc gagcatatcg tcacggttga
    13441 atcagaactc cccaccgcgg cacgttttga atgggtgcat cgtcatttgc cggggctgat
    13501 cacccttaat gcttaccgca cgctcaccgt tctgcacgac cccgccactt tacgctttgg
    13561 ttgggctaat aaacatatca ttaagaattt gcatcgtgat gaagtcctgg cacagctgga
    13621 aaaaagcctg aaatcaccac gcagtgtcgc accgtggacg cgcgaggagt ggcaaagaaa
    13681 actggagcga gagtatcagg atatcgctgc cctgccacag aacgcgaagt taaaaatcaa
    13741 acgtccggtg aaggtgcagc cgattgcccg cgtctggtac aaaggagatc aaaaacaagt
    13801 ccaacacgcc tgccctacac cgctgattgc actgattaat cgggataatg gcgcgggcgt
    13861 gccggacgtt ggtgagttgt taaattacga tgccgacaat gtgcagcacc gttataaacc
    13921 acaggcgcag ccgcttcgtt tgatcattcc acggctgcac ctgtatgttg cggattaacg
    13981 cccggctttc atactgccga ccatctgttc tggccgtacc cagctgtcaa actcggcttc
    14041 gctaagatac cccagcgcaa gggccgcagc ttttaaggtc agcccttctt tatgcgcttt
    14101 tttggcgatc tccgcggctt tgtcataacc aatgtgggtg ttaagcgcag tcaccagcat
    14161 cagcgattca ttgagtaatt gattgattcg ctcacgattc ggttcaatac ccactgcgca
    14221 gtgtttgtta aaactttcca tgccatctgc cagcaagcgc accgattgca ggaaattgtg
    14281 gatcaccatt ggacggaaga cgttcagttc aaagttaccg gaagcgccac ccatgttgat
    14341 cgccacgtcg ttccccatta cctgacagca gagcatggtt aatgcttcac actgggttgg
    14401 gttcactttc cccggcatga ttgagctgcc cggctcattt tccgggattg agatttcacc
    14461 aattccgcag cgcgggccag aggccagcca gcggacatca ttggcgattt tcatcagtga
    14521 cgcagccaac cctttcaacg cgccgtgcgc ctgaaccagg gcatcacagg tcgcaagcgc
    14581 ttcaaatttg ttcggcgcgg taacaaacgg tgcacaggta atgactgcca gttcatctgc
    14641 tacgcgacgc gcatactccg gatgggtatt tagtccagta cccaccgctg taccgccgag
    14701 agccagttcc gctacgtgag gcaggctgta ttcgatatgt ttgagattat gctcgagcat
    14761 cgctacccag ccggaaatct cctgccccag tgttagcggc gtggcgtcct gcaagtgggt
    14821 tcgaccgatt ttgacgatat cggcaaatgc acgcgatttt tcactcagtg tctgtgtcag
    14881 ggttttaacc tgcggaatga gttgcttgcg cagcgccagc agcgccgcaa cgtgcatcgc
    14941 cgtcggaaag acatcgttgg aactttggct tttgttcacg tcgtcgttag ggtgaacttt
    15001 acgttccatc ccgcgcacgc cgccgagtaa ttcactggcc cggttagcca gcacttcgtt
    15061 catgttcatg ttactttgcg tgccggagcc ggtctgccag atagccagcg ggaattcgtc
    15121 gtcatgctgt cctgccagta cttcatccgc cgcctgccga atggcgctcg ctttctcttc
    15181 agacaacaag cctaaatctt cattaacttt tgccgctgcg cgcttggtta gcgccagcgc
    15241 atgaatcagt gaggtgggca ttttctccgt cgaaatgcgg aaatgctcca gcgagcgttg
    15301 agtttgtgcg ccccacagct tatctgccgg gacatcaatc gcccccatcg aatctttttc
    15361 gctgcgtact gtattcatgg cctgctcctc acctgattaa ttttttcttt ctgttttgcc
    15421 ttcgttaagc aacttttagc tcacttatta tttactattt gataacaaat gtttggtctt
    15481 tcgtgccatg taaaaaaacc gccccgaagg gcggctctgt ttatttcaca cagcgagtgc
    15541 actgtgtgag ttgtatctgc tggaagaagt catttccttt atcatccaca aggataaacg
    15601 ccgggaaatc ttccacttca attttccaga tggcttccat tcccagttcc ggatattcaa
    15661 cacattccag gctcttaata cttccctgcg ccaatacagc ggccggacca ccgatactgc
    15721 caaggtagaa gccgccgtgt tttttacagg catccgtcac ctgctggctg cggttgcctt
    15781 tcgccagcat gatcatactt ccgccctgcg cttgcagttg atcgacataa gaatccatcc
    15841 gtccggcggt cgttgggcca agagaaccgg aggcataacc ttccggcgtt ttggccggac
    15901 ccgcgtagta aatcggatga tctttaatgt actgcggcag cccttcaccg ttatccatcc
    15961 gctctttcag tttggcgtga gcaatatcac gaccgacgat aatcgtgccg ttaagcgata
    16021 agcgtgtaga aacgggatac tgcgacaact gtgcgaggat ctctttcatc ggacggttaa
    16081 ggtcaacgcg caccgcttcg ccttctcccg ctttacgcag ctcttcaggg atatatttac
    16141 ctggattatg ttccagtttt tcgatccaga tcccctgacg gttgatcttc gctttgatat
    16201 tacggtcagc agagcaggaa acacccatac caaccggaca ggatgcgccg tgacgtggca
    16261 ggcgaatcac gcgtatgtcg tgagcgaagt atttaccacc aaactgcgca cccagaccga
    16321 gattttgcgc ttcgatcagc aattcttttt ccagttccac atcgcggaat gcctgaccgt
    16381 gctcattccc ttccgttggc agttcgtcat agtatttagc ggaagccagt ttcactgttt
    16441 taaggttagt ttctgcagaa gttccaccaa ttacgaacgc aatatgatac ggaggacagg
    16501 ccgccgtacc cagcgtgcgc atcttctcaa ccaggtaatt tttcagtttc cccggcgtta
    16561 gtaacgcttt ggtttcctga tagagatacg tcttgtttgc cgaaccacca cctttggcga
    16621 tacagaggaa tttgtactcg tcgccatcaa cggcataaag atcgatctgc gctggcagat
    16681 tggtgccggt attcacctct ttatacatat ccagcggcgc gttttgcgag tagcgcaaat
    16741 tatcttcgat ataagtgtta tagacaccgc gcgccagcgc cgcttcatca cagccaccgg
    16801 tccatacacg ctgccctttt ttaccaacaa taatcgcagt gccggtatcc tgacaggttg
    16861 gcagaacacc tttcgccgcg atgtcggagt tgcgcaggaa ttgcagcgcc acatatttat
    16921 cattttcgct ggcttccggg tcacgcagaa tgtcggccac ctgttgttgg tgagccggac
    16981 gcagcataaa tgacgcatca tgaaacgcct gacgcgccaa cagagttaac gcttcgggtg
    17041 cgactttcaa aatctcctgc ccttcaaatt cagatacgct aacgtgttcg ctggttagca
    17101 ggtaatactc agtatcatct tttttgagtg gaaaaggagc ctgataatga aagggtttgt
    17161 ttgacattgt tctctcactt actgcctggt ttggttatgc tctgggcggg tgttccgttg
    17221 ccctgttaaa agcgagtaac aatatcctac acactttttt aacaaaaact gagactagta
    17281 cgactttttg cggctccagg ttacttcccg taggattctt gctttaatag tgggattaat
    17341 ttccacatta aaacagggat tgatcatgca aaaactcatt aactcagtgc aaaactatgc
    17401 ctggggcagc aaaacggcgt tgactgaact ttatggtatg gaaaatccgt ccagccagcc
    17461 gatggccgag ctgtggatgg gcgcacatcc gaaaagcagt tcacgagtgc agaatgccgc
    17521 cggagatatc gtttcactgc gtgatgtgat tgagagtgat aaatcgactc tgctcggaga
    17581 ggccgttgcc aaacgctttg gcgaactgcc tttcctgttc aaagtattat gcgcagcaca
    17641 gccgctctcc attcaggttc atccaaacaa acgcaattct gaaatcggtt ttgccaaaga
    17701 aaatgccgca ggtatcccga tggatgccgc cgagcgtaac tataaagatc ctaaccacaa
    17761 gccggagctg gtttttgcgc tgacgccttt ccttgcgatg aacgcgtttc gtgagttttc
    17821 cgagattgtc tccctactcc agccagtcgc aggtgcacat ccggcaattg ctcacttttt
    17881 acaacagcct aatgccgaac gtttaagcga actgttcgcc agcctgttga atatgcaggg
    17941 tgaagaaaaa tcccgcgcgc tggcgatttt aaaatcggcg ctcgacagtc agcagggtga
    18001 accgtggcaa acgattcgtt taatttctga attttacccg gaagacagtg gcctgttctc
    18061 cccgctgtta ctgaatgtgg tgaaattgaa tcctggcgaa gcgatgttcc tgttcgctga
    18121 aacaccgcac gcttacctgc aaggcgtggc gctggaagtg atggcgaact ccgataacgt
    18181 gctgcgtgcg ggtctgacgc ctaagtacat tgatattccg gaactggttg ccaatgtgaa
    18241 attcgaagcc aaaccggcta accagttgtt gacccagccg gtgaaacaag gtgcagaact
    18301 ggacttcccg attccagtgg atgattttgc cttctcgctg catgacctta gtgataaaga
    18361 aaccaccatt agccagcaga gtgccgccat tttgttctgc gtcgaaggcg atgcaacgtt
    18421 gtggaaaggt tctcagcagt tacagcttaa accgggtgaa tcagcgttta ttgccgccaa
    18481 cgaatcaccg gtgactgtca aaggccacgg ccgtttagcg cgtgtttaca acaagctgta
    18541 agagcttact gaaaaaatta acatctcttg ctaagcttat taaaggctta taacaccttc
    18601 aggcggccag tccgcctgat ttcattttat ggataatcat tatgaataaa tcgctggtag
    18661 cggtaggcgt cattgttgcg ctaggcgtag tctggacagg cggcgcatgg tatacaggca
    18721 agaagattga aacccatctc gaagacatgg tcgcgcaggc gaacgcgcaa ctcaaactga
    18781 cagctcctga atccaacctg gaagtgagtt atcaaaacta tcatcgcggc gtattcagca
    18841 gccagttgca actgttggtg aaacccattg ccgggaaaga aaatccgtgg attaaaagcg
    18901 gtcagagcgt catcttcaac gaatcggttg atcatggtcc cttcccgctt gcccagctta
    18961 aaaaactgaa cctgatcccg tcgatggcat caattcaaac cacgctggtt aataacgaag
    19021 taagcaaacc actgtttgat atggcaaaag gtgaaacgcc ttttgagatt aactcgcgca
    19081 ttggttacag cggtgattcc agttccgata tttcgctcaa gccactgaat tacgagcaaa
    19141 aggatgaaaa agtcgccttt agcggcggcg agttccagtt aaatgctgac agagacggca
    19201 aagccatctc cctttccggg gaggcgcaaa gtggtcggat agacgcagtt aacgaataca
    19261 accagaaagt gcagttgacc tttaataatc tgaaaaccga cggttccagc acgctggcaa
    19321 gttttggtga gcgcgtagga aaccaaaaac tgtcactgga aaaaatgacc atttcagtgg
    19381 aaggcaaaga actggcactg ctggaaggca tggagatcag cggtaaatcg gatctggtca
    19441 atgacggtaa aacgatcaat agccaactgg attactcgct aaacagcctg aaggtacaga
    19501 atcaggatct gggcagcggc aagctgactt taaaagtcgg ccagattgat ggtgaagcct
    19561 ggcatcagtt tagccagcaa tataacgcgc aaactcaggc gctgctggca cagccagaaa
    19621 ttgccaacaa tcccgaactt tatcaggaga aagtgacgga agccttcttt agcgccctgc
    19681 cgctgatgtt gaaaggcgat ccggtgatta ctatcgcgcc gctaagctgg aaaaacagtc
    19741 agggtgaaag tgcgctgaat ctgtcgctgt tcctgaaaga tccggcaacg actaaagaag
    19801 cgccgcaaac gctggcgcag gaagtagatc gttcggttaa atctctggat gcgaaactga
    19861 ccattccggt ggatatggca actgagttta tgactcaggt agcgaagctg gaaggttatc
    19921 aggaagatca agcgaaaaaa ctggcgaaac agcaagttga aggtgcatca gcaatggggc
    19981 agatgttccg tctgaccacc ttgcaggaca ataccatcac caccagcctg caatatacta
    20041 acggtcagat aacgttaaac gggcagaaaa tgccactgga agatttcgtt ggtatgtttg
    20101 cgatgccagc tcttaacgtt ccggctgtac cggcaattcc gcagcagtaa ttcacggcca
    20161 aaacgccgga gaattttcac ttcggcgttt tcatctgaaa acaaaccaga gattgcctga
    20221 tgcgctacgc ttatcaggcc taccttttcc tgcaacactt tgaatttatg agtttttgta
    20281 ggctggataa ggcgtttaca ccgcatccgg cataaaaaac gcgcactttg tcaacaatct
    20341 gaaacgccgg aggtttttct ctccggcgtt atttctgact tcagcataaa gtcatactga
    20401 atactcattt tcacatccca gtaattcagg cgatcgccca gcgtactgcc atcgcgcttt
    20461 tcccgccctt tatcggcagc aagaaagaaa ttcatccctt gtaacggccc ttcgctgacc
    20521 cgatagttca taccacctaa gatacccgcg ccttcaatcc gcacatctcg tttactggat
    20581 tcatagccgc gagtcacaat ctgtgcaaat gtcagtgtaa attgcggcgt taaacgatag
    20641 ttgacgccca attgccagga ctgcattcct tcacggttac gatcaagact taatgaaaag
    20701 ggatagccaa tgtcggtatc caccacggca ccattcattg catggcgcaa ataaccacca
    20761 ctgccaaata cgcttcccca cggcgcattc caggtcaatt gtccactaac caacgccgtt
    20821 tcattgggct ggctggtatt acgacttaaa ccttcgagct gcgcgtaaaa gccgagcaac
    20881 tcccctttca acgtataatt cgggtcgagc ggtatgcttt tctcgagaat actttgtatc
    20941 tgattggaaa catctttctg taccgatgcc cccagagaat agcgactggt atcatcatgc
    21001 caactggcgg ctaaaaaaga acgtgcgcga tgtttatcgt attttcccgt ctggctgtta
    21061 tacacccctt catcacgaat accggttgtt gaatcagtcc ataaaaatac tcgtgaacgc
    21121 cacattcccc acagatcaat atttggtgtt aaagcccaat aacctaatgc tccttcagaa
    21181 actgtcggta atgtacgttg cgaactgcca gccattaacg gaatacgata aggtaagcta
    21241 ccataactat aatccgtccc gaagcgacct gatttaagca ggctattttc actgggtttt
    21301 atttttactg cccccagggc aaagccaaaa cttttatcac catcaagata ccaccgggta
    21361 ctcatatcag cacgagcacc taatttataa acataatatg ccccgccttc aacgccaata
    21421 atattggaat aataaccact attgaaatcg agcaatcctc cctgtaccca tgcgtaaata
    21481 tctcttcccg cgccagcact gggcttatcc gccctgcgca attcgttttt cagtctgatg
    21541 cgtagtcccg cctcatcgtc agccagttgc gccgcataag cagagataag gccagaggca
    21601 gccgtcaggc aaataacggc catggccact atttttctca tagttgcacc tttgatgttc
    21661 tgattatttt tattgattat taattagtga tatcgctgat taattgctgc tgcacttttt
    21721 tacgattatc aatttcaacc acgatttctt tgaatttttt atccgtaagc ggataaaacc
    21781 agataataac gaatgccagt agcataaatc cgcaaggtac taaggcaatt gatgtgcgga
    21841 tgcccataat aacttccggc gtttgcacct gattggcaat atatccgctt aaccccaaaa
    21901 taaaggcagg aattgaacct ccgattgcct gaccgcattt acgggtaaat gagaatagtg
    21961 aataggtgag cccttcaatt cgcacgccgg tcaggtattc accgtattct acggtatcag
    22021 cttccagtgc ccacatcact gtcatagtaa cgccctgacc aattgaagcg atggccaacg
    22081 caaccaacgc caccggcagt gaccagacgg aaacccagaa gaatagcaga taaccgcagg
    22141 ttcccagcaa agcgccaatc aggaaggtat tctttttacc gatcctcgcg accatccccg
    22201 gcacaagcgg tgccgatgcc acagtaccaa ccaggttttg caccagtacc agcacagtga
    22261 acagcccggt atcatttaac acatagcgca cgtagaacaa cgacgaggcg ctgaccgcaa
    22321 aggtcgaaat cagcacacac agcgcaccga tgcacaacat aaacagcggg cgattccgtt
    22381 tcagggtttg caaactgatc ttcaatgacg gctgcgcaac gatacgtacc acattctcac
    22441 gcgtcgattt gaagcagatg aagtaaagca ccattccggc aatcgccagc acaatcgtcc
    22501 agaaatggta taccgacacc atctcttccg ggctggagtt cttaatgctc ggccctatca
    22561 gaaatgccag gcagacaaag gtcaatgaag cggcaatccc acgagccgcg cccagacggg
    22621 cgcgggattg tggttgttgg gtcatcgcgg tagcaagtga accataagga atattcacca
    22681 ggctgtagca aagcccgagg cccatgtagg tcaaatatgc atacactact ttgcggctat
    22741 ggctccagtc ggtcggtacc cagaatacca gcacgctgaa gatcattaac ggcgcagtac
    22801 cgaagagtaa aaacgggcgg aattttcccc agcgggtatt cacactgtcc accactcgtc
    22861 cggcaaagac gtcggcgaag gcatcgaata cccgcaccag taacagcatg gtgcccgccg
    22921 cagcggcacc gacgccagcg acgtcggtgt agtaactcaa caggaagagc acccccattg
    22981 cgaaggcgaa gttattggcg acgtcaccga ggctgtagcc gacgatggtg cgccaggaga
    23041 gttgttgatt cattgtttgc ctccctgctg cggtttttca ccgaagttca tgccagtcca
    23101 gcgtttttgc agcagaaaag ccgccgactt cggtttgcgg tcgcgggtga agatcccctt
    23161 cttgttaccg ccaacgcgca atatgccttg cgaggtcgca aaatcggcga aattccatac
    23221 ctgttcaccg acgacggcgc tgacgcgatc aaagacgcgg tgatacatat ccagccatgc
    23281 acactgatac tcttcactcc acatgtcggt gtacattgag tgcagcccgg ctaacgtatc
    23341 cacgccgtat tcggtgatga taatcggctg atgcagtttc tcctgccagg ccagaagttc
    23401 tttttccagt accttctctg ccgtttccaa atcgccgctt tggacatacc atccgtaata
    23461 acggttcagg cacagcacat caaagagatc gctgatggta tcggtgtgag cgtcgcagaa
    23521 cattacattg acgcaggtga tcggacgcgt cgggtcgagt ttacgcgttg cttccgccag
    23581 tggcgcgaaa tattcccgtg caccttgcgg acgggtatcc ggttcgttgg caatactcca
    23641 catcaccacg cttgggtggt ttttgtcacg cgctatcagc tctttaatcg cctgtaagtg
    23701 cgcttgctga gtttccccgt tgactgcctc ttcgctgtac agttctttcg gcttgttgcc
    23761 cgcttcgaaa ccaatgccta aagagaggtt aaagccgaca gcagcagttt catcaatcac
    23821 cacgatgcca tgttcatctg cccagtcgag catctcttca gcgtaagggt aatgcgaggt
    23881 acggtaggag ttggccccaa tccagtccat taatgcgtgg tcgtgcacca tcagcacgtt
    23941 atcgaatcct ttgccacgca agtccgcatc ttcatgacga ccaaagccag taaagtagaa
    24001 cggtttgtgg ttaatcagga actgttcgcc cttcactgcc actgaccgga tgccgacgcg
    24061 aagcgggtag atatcacact ctgtctggct tttggctgtg acgcacagtt catagagata
    24121 accttcaccc ggttgccaga ggtgcggatt caccacttgc aaagtcccgc tagtgccttg
    24181 tccagttgca accacctgtt gatccgcatc acgcagttca acgctgacat caccattggc
    24241 caccacctgc cagtcaacag acgcgtggtt acagtcttgc gcgacatgcg tcaccacggt
    24301 gatatcgtcc acccaggtgt tcggcgtggt gtagagcatt acgctgcgat ggatcccggc
    24361 atagttaaag aaatcatgga agtaagactg ctttttcttg ccgttttcgt cggtaatcac
    24421 cattcccggc gggatagtct gccagttcag ttcgttgttc acacaaacgg tgatacgtac
    24481 acttttcccg gcaataacat acggcgtgac atcggcttca aatggcgtat agccgccctg
    24541 atgctccatc acttcctgat tattgaccca cactttgccg taatgagtga ccgcatcgaa
    24601 acgcagcacg atacgctggc ctgcccaacc tttcggtata aagacttcgc gctgatacca
    24661 gacgttgccc gcataattac gaatatctgc atcggcgaac tgatcgttaa aactgcctgg
    24721 cacagcaatt gcccggcttt cttgtaacgc gctttcccac caacgctgat caattccaca
    24781 gttttcgcga tccagactga atgcccacag gccgtcgagt tttttgattt cacgggttgg
    24841 ggtttctaca ggacgtaaca taagggactc ctcattaaga taaaaatact ggtcaacctt
    24901 taatctgatt agttaaccaa ttctgtgttg cacacagtta tctgtgagaa acccatggaa
    24961 gagaaatgga aataaaaaga actgacaccg cgatcaaaaa accaaaaact gcaataccca
    25021 cagcgtgata ttgatcgcat taatctttaa aacggcgttg acagaagcca taaaataagc
    25081 aaccgaacgt tcgttcgagc atattgggaa cgataaactc tcatgacata tggctacggt
    25141 gaatattttt gggggggaat tctccgatcc cggctagatt gtattttgta cagggaaatt
    25201 aaagtatgaa acgcgggcac agttatcacc agcccgcgaa atgagaggat caggatgcgg
    25261 ttaagatacc gccaatcatc gcgcgtaatc cctgagcaaa cgacgcttca cgcgccagat
    25321 cttccgcttc gatatcggcc agtacaccgt aggtcagatc cagcagtaaa cgtgaagtca
    25381 ttgacgtgtt gatgtctggg cttatctcgc ctttttgctg ggcatcacgc atccgctggg
    25441 caacaaattc cgtgatcgtc atatgcttat ttttaagcat ggcggcgacc tgtgggttac
    25501 gcatcccttc cgccataatt tcgaccacca gcgcccgttg cccaaaggct tcatgggtga
    25561 gagagacaat agactcgacc atatagtcaa caaaatgaat ccctttaatc ggttcccgga
    25621 aacgagccag cgccctctcc tggtcctgta agataatcgc ctgaatcaag gcttctttgg
    25681 agatgaaatg gtgatagagc gtcccgggac taatggcgca agatttacag atggctttca
    25741 tcgaggcact gtgaaatcca ttttctgaaa aaatctctct ggcagcattg aggatccggg
    25801 tccgtgtcgg ttgtgcttca gtctgcatgt tatccatcat taaaccatta tagttagcgt
    25861 gaataactag tttaccgcgt tgtcaggtga gcgcctatca ttgctgttga atagtgcgaa
    25921 ggcacactct attcgtatac ttttcactct gaaattggtt aaccaatagc cgtgatttgg
    25981 ctgccagtta tttagcagag acgttgatga gtttatcccg gtaaacaaca cgcacggttg
    26041 tttaccggtc cgttagtgta ttaattgagc tcctgtaccc caccaccgga gacggtgaga
    26101 atttgtccgc ttacccagct cgcagcaggc gagcaaagga acagcgctgc gttagcaata
    26161 tcttgcggtt ggcccagacg tctgatcggc gtgtgctgta acattttttg ttcaatttct
    26221 ggtgtaataa cggatttcag ggcatcggtt aatattgccc ccggcgcaat gccatttacc
    26281 cgaatatttt tttcacccag gtcaaacgcc atatttctga ccagatgact ggctgcagct
    26341 ttagatgatg cataggaagt catgtttata tttttatttt ctgccgccat agaagtgatg
    26401 gtcagaataa cgccaccgcc atttttttcc atttctggcg caacaagttg tgacagatgg
    26461 aaaaaagaaa acacattcag ttcataagca cggcgaaaat ccgccattgg catatcaaac
    26521 ggtttaggtc caccgccacc ggcgttgtta accagaatat caactttacc cagcttactg
    26581 atagcaaagt ctgccagtgc agagagttcc tgttcggaag taatatcaca acggcaggca
    26641 aatgcctgac cacccagttg ttgaatttcg tctacaacat ggttagctgc gtcggcgtta
    26701 atatcactga ccaccacaga tgcgccagct gtcgcgaatg taatggcgat ttctttacca
    26761 atacctgcac ccgcacctgt gatgatggcg catggcgcat ggcgcatggc gcatggcgca
    26821 tggcgcatgg cgcatggcgc atggcgcatg gcgcattttc cgtc
//
LOCUS       DADMKF010000047        26558 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.048, whole genome shotgun sequence.
ACCESSION   DADMKF010000047 DADMKF010000000
VERSION     DADMKF010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26558
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.048"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            58..381
                     /gene="iraM"
                     /locus_tag="JNC24_001779"
     CDS             58..381
                     /gene="iraM"
                     /locus_tag="JNC24_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HAY9158536.1"
                     /translation="MKWVVIDTVIQPTCGISFSAIWGDMKKIIWYQSTIFLPPGSIFT
                     PVKLGIILKEKEYPITIYNIAPFNKNLWSLLKSSQECPPGESKITNKCLHNSCIIKIC
                     PYGLK"
     gene            complement(484..1734)
                     /gene="icd"
                     /locus_tag="JNC24_001780"
     CDS             complement(484..1734)
                     /gene="icd"
                     /locus_tag="JNC24_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HAY9158537.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGEAIIENM"
     gene            1906..2559
                     /gene="rluE"
                     /locus_tag="JNC24_001781"
     CDS             1906..2559
                     /gene="rluE"
                     /locus_tag="JNC24_001781"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309634.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HAY9158538.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKPENQPTRVILF
                     NKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDTEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYTLDNLANGEWREATE"
     gene            2569..3030
                     /gene="nudJ"
                     /locus_tag="JNC24_001782"
     CDS             2569..3030
                     /gene="nudJ"
                     /locus_tag="JNC24_001782"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HAY9158539.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILKASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            3084..4190
                     /gene="mnmA"
                     /locus_tag="JNC24_001783"
     CDS             3084..4190
                     /gene="mnmA"
                     /locus_tag="JNC24_001783"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HAY9158540.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPGQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIEVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            4226..4867
                     /gene="hflD"
                     /locus_tag="JNC24_001784"
     CDS             4226..4867
                     /gene="hflD"
                     /locus_tag="JNC24_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HAY9158541.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSRQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            4871..6241
                     /gene="purB"
                     /locus_tag="JNC24_001785"
     CDS             4871..6241
                     /gene="purB"
                     /locus_tag="JNC24_001785"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HAY9158542.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQYRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            6409..7080
                     /gene="phoP"
                     /locus_tag="JNC24_001786"
     CDS             6409..7080
                     /gene="phoP"
                     /locus_tag="JNC24_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HAY9158543.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            7080..8540
                     /gene="phoQ"
                     /locus_tag="JNC24_001787"
     CDS             7080..8540
                     /gene="phoQ"
                     /locus_tag="JNC24_001787"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HAY9158544.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAKWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            8616..9737
                     /locus_tag="JNC24_001788"
     CDS             8616..9737
                     /locus_tag="JNC24_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HAY9158545.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(9786..11012)
                     /gene="pepT"
                     /locus_tag="JNC24_001789"
     CDS             complement(9786..11012)
                     /gene="pepT"
                     /locus_tag="JNC24_001789"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HAY9158546.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVTLEGMEK
                     AVQVIVRIAELTAQRK"
     gene            11262..12398
                     /gene="potA"
                     /locus_tag="JNC24_001790"
     CDS             11262..12398
                     /gene="potA"
                     /locus_tag="JNC24_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HAY9158547.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMREGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            12382..13239
                     /gene="potB"
                     /locus_tag="JNC24_001791"
     CDS             12382..13239
                     /gene="potB"
                     /locus_tag="JNC24_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HAY9158548.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            13236..14030
                     /gene="potC"
                     /locus_tag="JNC24_001792"
     CDS             13236..14030
                     /gene="potC"
                     /locus_tag="JNC24_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HAY9158549.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            14027..15073
                     /gene="potD"
                     /locus_tag="JNC24_001793"
     CDS             14027..15073
                     /gene="potD"
                     /locus_tag="JNC24_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HAY9158550.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKAIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            complement(15229..16050)
                     /gene="cobB"
                     /locus_tag="JNC24_001794"
     CDS             complement(15229..16050)
                     /gene="cobB"
                     /locus_tag="JNC24_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HAY9158551.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADIFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLKGL"
     gene            complement(16066..16977)
                     /gene="nagK"
                     /locus_tag="JNC24_001795"
     CDS             complement(16066..16977)
                     /gene="nagK"
                     /locus_tag="JNC24_001795"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HAY9158552.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGTLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLIFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQIGCIENYLSGRGFAWLWQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVCLGNILTIVDSDLVVIGGGLSNFPAITTQLADRLPRHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(17006..18250)
                     /gene="lolE"
                     /locus_tag="JNC24_001796"
     CDS             complement(17006..18250)
                     /gene="lolE"
                     /locus_tag="JNC24_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HAY9158553.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVNQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGTNLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHIAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VIIGVVVSLQLTPIIERIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(18250..18951)
                     /gene="lolD"
                     /locus_tag="JNC24_001797"
     CDS             complement(18250..18951)
                     /gene="lolD"
                     /locus_tag="JNC24_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HAY9158554.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(18944..20143)
                     /gene="lolC"
                     /locus_tag="JNC24_001798"
     CDS             complement(18944..20143)
                     /gene="lolC"
                     /locus_tag="JNC24_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HAY9158555.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGNFAANSEVDGYEMLVNIEDASRLMRYPAGN
                     ITGWRLWLDEPLKVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            20405..21478
                     /locus_tag="JNC24_001799"
     CDS             20405..21478
                     /locus_tag="JNC24_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HAY9158556.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHMTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            21606..25052
                     /gene="mfd"
                     /locus_tag="JNC24_001800"
     CDS             21606..25052
                     /gene="mfd"
                     /locus_tag="JNC24_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HAY9158557.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKVRNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            25196..26152
                     /gene="ldtC"
                     /locus_tag="JNC24_001801"
                     /pseudo
     CDS             25196..26152
                     /gene="ldtC"
                     /locus_tag="JNC24_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707028.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
     gene            complement(26235..26492)
                     /gene="bhsA"
                     /locus_tag="JNC24_001802"
     CDS             complement(26235..26492)
                     /gene="bhsA"
                     /locus_tag="JNC24_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HAY9158558.1"
                     /translation="MKNVKTLIAAAILSSMSFANFAAVEVQSTPEGQQKVGTISANAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
BASE COUNT         6498 a         6883 c         6719 g         6458 t
ORIGIN      
        1 taaagaaatg aacatccaaa tgaactatat taaataccgt gggataagac ataacaaatg
       61 aagtgggtag taattgacac ggtaattcaa cctacatgcg gcatatcttt ttcagccata
      121 tggggtgata tgaaaaagat catctggtat caatctacta tatttcttcc tcctggaagt
      181 atatttacac cggttaagct tggtattatc cttaaggaaa aagaatatcc tattactatt
      241 tataacattg caccattcaa caagaattta tggagtttac tcaaaagcag tcaagagtgt
      301 cctccaggag aaagcaaaat aacaaataaa tgtttacata atagttgcat tataaaaata
      361 tgcccatatg ggctcaagta atgggcttca tataaatatg ctcgatatgc tattaataat
      421 atccacataa ataaaacaac gggcgtgtta tacgcccgtt tcaatattta actcatggag
      481 agattacatg ttttcgatga tcgcttcacc aaactctgaa catttcagca gcttagcgcc
      541 ttccatcaga cgttcaaagt cataagttac ggttttcgcg ttgatagcac cttccatacc
      601 tttaacaatt aaatcagccg cttcagtcca ccccatatgg cgcagcatca tctcagcgga
      661 gaggatgata gagcctggat ttactttgtc ctgaccggca tatttcggcg cagtaccgtg
      721 ggtggcttca aacagggcgc attcgtcacc gatgtttgcg ccaggggcga taccgatacc
      781 gccaacctgc gctgccaggg cgtcagaaat gtagtcaccg ttcaggttca tacaggcgat
      841 aacatcatat tcagccggac gcagcaggat ctgttgcagg aatgcatcag caatcacgtc
      901 tttaatgacg atctctttgc cggtgttcgg gtttttaact ttcagccacg ggccgccgtc
      961 gatcagttca ccgccaaact cttcacgcgc cagctggtag ccccagtctt taaacgcgcc
     1021 ttcggtgaac ttcatgatgt tgcctttgtg caccagagtc acagagtcac gatcgttagc
     1081 aattgcgtat tcgatcgctg cacgaaccag acgtttggtg ccttcttccg aacacggctt
     1141 aataccgata ccacaatgtt ccgggaagcg aattttcttc acccccatct cttcacgcag
     1201 gaatttaatc actttctcgg cgtcggcaga gtctgctttc cattcgatac ccgcataaat
     1261 gtcttccgag ttttcacgga agataaccat atcggtcagt tcagggtgtt taaccgggct
     1321 tggagtgccc tgatagtaac gtaccggacg caggcagatg tagagatcca gttcctggcg
     1381 cagggcaacg ttcagagagc gaataccgcc accaacagga gtggtcagcg gacctttaat
     1441 ggcaacgcga tattcacgaa tcagatcaag ggtttcagca ggcagccaga catcctgacc
     1501 ataaacctgt gtggattttt caccggtgta aatttccatc caggagattt tacgctcgcc
     1561 tttataggct ttctcgactg cagcgtcgac cactttcagc atggctgggg ttacatctac
     1621 accgattcca tcaccttcaa tgtaagggat aatcggattt tcaggaacgt tgagtttgcc
     1681 gttttgcagg gtgatcttct tgccttgtgc cggaacaact actttacttt ccattcacct
     1741 ctccttcgag cgctactggt ttgctcgtct gccaccacgt tgcatatgcg tttgcgtcct
     1801 gcgatacgga tgctttagag caattttttg ttaatgattt gtaattggct tgtcaatgct
     1861 accctattgt ttgccgtcat gaaagatgcg cgttattagg ctataatgcg gcaattcata
     1921 atctctgaaa ataccatgca aaaaacttct tttagaaatc accaggttaa gcgattcagc
     1981 tcgcaacgtt ctactaggcg taaacctgaa aaccagccca cgcgtgtgat cctgttcaat
     2041 aaaccctacg atgttcttcc gcagttcacc gatgaagccg gacgcaaaac attaaaagaa
     2101 ttcatcccgg ttcagggcgt ttatgcagca ggtcgtcttg accgcgatac cgaagggtta
     2161 ctggtgctga ccaataacgg cgcgttgcag gcgcgtttaa cccagccggg caaacgcacc
     2221 gggaaaatct attatgtgca ggtggaaggt attcccacac aagacgcact tgaagccttg
     2281 cgcaatggcg taaccttaaa tgatggccct accctgcccg ccggcgcgga gctggttgac
     2341 gaaccagcgt ggttatggcc gcggaatcca ccaattcgtg aacgcaaaag tattcccacc
     2401 agctggctga agatcacctt atatgaagga cgtaatcgcc aggtgcgccg catgaccgcc
     2461 catgttggtt tccccacgct gcgactgatt cgttatgcga tgggtgatta cactttggat
     2521 aaccttgcca atggcgaatg gcgagaagca acagaataag gatgcacaat gtttaaaccg
     2581 cacgttaccg ttgcttgcgt agtgcacgca gagggtaaat ttttagtcgt tgaagagacg
     2641 attaatggta aagcgttatg gaaccaacct gccgggcatc tggaagccga tgaaacctta
     2701 gtggaagctg ccgcccgtga gctatgggaa gaaaccggca tcagcgcgca accgcaacac
     2761 tttattcgta tgcatcagtg gattgcgcca gataaaacgc cgtttttgcg tttcctcttt
     2821 gccattgagc ttgagcaaat atgcccgacc cagcctcatg acagcgatat cgactgctgt
     2881 cgttgggtca gcgccgaaga aattttaaag gcgtcaaatc ttcgttcgcc gctggtggcg
     2941 gaaagtatcc gttgttatca aagtgggcaa cgctatccgc tggagatgat tggcgatttt
     3001 aactggcctt ttacaaaggg tgtcatctaa aaggctgcgt gatagaatac gccgccttga
     3061 agttcaatgt cgtgagtgat ccaatgtctg aaaccgcaaa aaaagtaatc gtcggcatgt
     3121 ccggcggtgt cgattcctcc gtttctgcct ggctgttgca acaacaggga tatcaggttg
     3181 aaggcctgtt tatgaagaac tgggaagaag acgacggtga ggaatattgc acagcggcag
     3241 cggatctggc tgatgcccag gctgtctgcg acaagctcgg cattgaactg cacaccgtta
     3301 actttgctgc cgagtactgg gacaatgtct tcgaactgtt ccttgccgaa tataaagccg
     3361 gtcgcacgcc gaatccggat attctgtgca acaaagagat caaatttaaa gctttcctcg
     3421 aatttgccgc cgaagattta ggtgccgatt atatcgctac cggtcattac gtacgtcggg
     3481 ccgatgtcga tggcaagagc cgcctgctgc gtggtctgga cagcaataaa gaccagagct
     3541 acttccttta tacgctcagc catgagcaga ttgcgcaaag cctgttcccg gtcggcgaac
     3601 tggaaaaacc gcaggtgcgt aagattgctg aagatcttgg tctggtcacc gcgaagaaaa
     3661 aagactctac cggcatttgc ttcattggcg aacgtaaatt ccgcgagttc ctgggccgtt
     3721 atctcccggg gcaaccgggc aaaatcatta ccgtcgatgg cgatgaaatt ggtgagcacc
     3781 aggggctgat gtatcacact ctcggtcagc gtaaaggtct gggtatcggt ggcaccaaag
     3841 aaggcaccga agaaccttgg tatgtggtgg acaaagacgt cgaaaacaac attctggttg
     3901 tcgctcaggg ccatgaacac ccgcggctga tgtctgtcgg gttgattgcc cagcagttgc
     3961 actgggtcga tcgcgaacca ttcaccggca ctatgcgttg cacggtaaaa acccgctatc
     4021 gccagaccga tatcccttgc accgtcaagg cgctggacga tgatcgcatt gaagtgattt
     4081 tcgatgaacc ggttgccgcc gtgacgccgg gccagtctgc cgtcttctat aacggtgaag
     4141 tgtgcctcgg tggcggtatt attgagcagc gtctgccgct gccggtctga ttattatctt
     4201 tacttaaaac aaggaagcag tgaacgtggc aaagaattac tatgacatca ccctcgccct
     4261 ggccggtatt tgtcagtcgg cacgcctggt gcaacaactc gctcaccagg ggcattgtga
     4321 tgccgatgcg ctacacgtct cactcaacag tattattgat atgaacccca gctcgacgct
     4381 ggcggttttt ggcggtagcg aagccaacct gcgcgtcggg ctggaaaccc tgctcggcgt
     4441 gctcaatgcc agcagtcgcc agggcttaaa cgccgaatta acccgctaca cactcagctt
     4501 gatggtgctt gagcgcaaac tctcctcagc gaaaggcgcg ctcgacactc tgggcaaccg
     4561 gatcaacggc ctgcaacgcc agctcgaaca cttcgattta cagtccgaaa cgctgatgag
     4621 cgcgatggct gctatctatg ttgatgtgat cagcccgctt ggcccacgca ttcaggtcac
     4681 cggttcccct gctgtactgc aaagcccaca agtgcaggcg aaagttcgcg caaccctgct
     4741 ggcaggcatt cgcgccgccg tgctctggca ccaggtcggc ggcggacgtc tgcaactgat
     4801 gttttctcgt aatcgcctga ccactcaggc aaaacaaatt cttgctcatt taaccccgga
     4861 gttgtgatct atggaattat cctcactgac cgccgtttcc cctgtcgatg gacgctacgg
     4921 cgataaagtc agcgcgctgc gcgggatttt cagcgaatat ggtttgctga aattccgtgt
     4981 acaagttgaa gtacgttggc tgcaaaaact ggccgcgcac gcagcgatca aggaagttcc
     5041 tgcttttgct gccgacgcaa tcggttacct tgatgcaatt gtcgccaatt tcagcgaaga
     5101 agatgccgca cgcatcaaaa ccatcgagcg taccactaac cacgacgtta aagcggttga
     5161 gtatttcctg aaagaaaaag tggcggagat cccggaactg cacgcggttt ctgaattcat
     5221 ccactttgcc tgtacttcgg aagatatcaa taacctctcc cacgcattaa tgctgaaaac
     5281 cgcgcgtgat gaagtgatcc tgccgtactg gcgtcaactg attgatggca ttaaagatct
     5341 cgccgttcag taccgcgata tcccgctgct gtctcgtacc cacggtcagc cagccacgcc
     5401 gtcaaccatc ggtaaagaga tggcaaacgt cgcctaccgt atggagcgcc agtaccgcca
     5461 gcttaaccag gtggagatcc tcggcaaaat caacggcgcg gtcggtaact ataacgccca
     5521 catcgccgct tacccggaag ttgactggca tcagttcagc gaagagttcg tcacctcgct
     5581 gggtattcaa tggaacccgt acactaccca gattgaaccg cacgactaca tcgccgagct
     5641 gtttgattgc gttgcacgct tcaacaccat tctgatcgac tttgatcgtg acgtctgggg
     5701 ttatatcgcc cttaaccact tcaaacagaa aaccattgct ggtgagattg gttcttccac
     5761 catgccgcat aaagttaacc cgatcgactt cgagaactcc gaagggaatc tgggcctttc
     5821 caacgcggta ttacagcatc tggcaagcaa actgccggtt tcccgctggc agcgtgacct
     5881 gaccgactcc accgtgctgc gtaacctcgg cgtgggtatc ggttatgcgc tgattgcgta
     5941 tcaatccacc ctgaaaggcg tgagcaaact ggaagtgaac cgtgaccatc tgctggatga
     6001 gctggatcac aactgggaag tgctggctga gccaatccag acagttatgc gtcgctatgg
     6061 catcgaaaaa ccgtacgaga agctgaaaga gctgactcgt ggtaagcgcg ttgacgccga
     6121 aggcatgaag cagtttatcg acggtctggc gttgccagaa gaagagaaag cccgcctgaa
     6181 agcgatgacg ccggcaaact acattggtcg cgccatcacc atggttgatg agctgaaata
     6241 aactcgtatc agtgccggat ggcgatgctg tccggcctgc ttattaagat tatccgcttt
     6301 ttattttttc actttacctc ccctccccgc tggtttattt aatgtttacc cccataacca
     6361 cataatcgcg ttacactatt ttaataatta agacagggag aaataaaaat gcgcgtactg
     6421 gttgttgaag acaatgcgtt gttacgtcac caccttaaag ttcagattca ggatgctggt
     6481 catcaggtcg atgatgcaga agatgccaaa gaagccgatt attatctcaa tgaacattta
     6541 ccggatattg cgattgtcga tctcggattg ccagacgagg acggtctgtc actgattcgc
     6601 cgctggcgta gcaatgatgt ttcactgccg attctggtat taaccgcccg tgaaagctgg
     6661 caggacaaag tcgaagtatt aagtgctggt gctgatgatt acgtgactaa accatttcat
     6721 attgaagagg tgatggcgcg aatgcaggca ttaatgcggc gtaatagcgg tctggcttca
     6781 caggtcattt cgctaccacc gtttcaggtt gatctctctc gccgtgaatt atctattaat
     6841 gacgaagtga tcaaactgac cgcgttcgaa tacaccatta tggaaacgtt gatacgcaat
     6901 aatggcaaag tggtgagcaa agattcgtta atgctccaac tctatccgga tgcggagctg
     6961 cgggaaagcc ataccattga tgtactgatg ggacgtctgc gcaaaaaaat tcaggcacaa
     7021 tatccgcaag aagtgattac taccgttcgc ggccagggct atttgttcga attgcgctga
     7081 tgaaaaaatt actgcgtctt tttttcccgc tctcgctgcg ggtacgtttt ctgttggcaa
     7141 cggctgcggt agtactggtg ctttcgcttg cctacggaat ggtcgcgctg atcggttata
     7201 gcgtcagttt cgataaaact acgtttcggc tgttacgtgg cgagagcaat ctgttctata
     7261 cccttgcgaa gtgggaaaac aataagttgc atgtcgagtt acccgaaaat atcgacaagc
     7321 aaagccccac catgacgcta atttatgatg agaacgggca gcttttatgg gcgcaacgtg
     7381 acgtgccctg gctgatgaag atgatccagc ctgactggct gaaatcgaat ggttttcatg
     7441 aaattgaagc ggatgttaac gataccagcc tcttgctgag tggagatcat tcgatacagc
     7501 aacagttgca ggaagtgcgg gaagatgatg acgacgcgga gatgacccac tcggtggcag
     7561 taaacgtcta cccggcaaca tcgcggatgc caaaattaac cattgtggtg gtggatacca
     7621 ttccggtgga gctaaaaagt tcctatatgg tctggagctg gtttatctat gtgctctcag
     7681 ccaatctgct gttagtgatc ccgctgctgt gggtcgccgc ctggtggagt ttacgcccca
     7741 tcgaagccct ggcaaaagaa gtccgcgaac tggaagaaca taaccgcgaa ttgctcaatc
     7801 cagccacaac gcgagaactg accagtctgg tacgaaacct gaaccgattg ttaaaaagtg
     7861 aacgcgaacg ttacgacaaa taccgtacga cgctcaccga cctgacccat agtctgaaaa
     7921 cgccactggc ggtgctgcaa agtacgctgc gttctctgcg tagtgaaaag atgagcgtca
     7981 gtgatgctga gccggtaatg ctggagcaaa tcagccgcat ttcacagcaa attggctact
     8041 acctgcatcg tgccagtatg cgcggcggga cattgctcag ccgcgagctg catccggtcg
     8101 ccccactgct ggacaatctc acctcagcgc tgaacaaagt gtatcaacgc aaaggggtca
     8161 atatctctct cgatatttcg ccagagatca gctttgtcgg tgagcagaac gattttgtcg
     8221 aggtgatggg caacgtgctg gataatgcct gtaaatattg cctcgagttt gtcgaaattt
     8281 ctgcaaggca aaccgacgag catctctata ttgtggtcga ggatgatggc cccggtattc
     8341 cattaagcaa gcgagaggtc attttcgacc gtggtcaacg ggttgatact ttacgccctg
     8401 ggcaaggtgt agggctggcg gtagcccgcg aaatcaccga gcaatatgag ggtaaaatcg
     8461 tcgccggaga gagcatgctg ggcggtgcgc ggatggaggt gatttttggt cgccagcatt
     8521 ctgcgccgaa agatgaataa atatgtccat acttcacgca ttacgttaag catccgttat
     8581 aatcggttgc agataccagc ctgtggatgc ttaacatgga ataccaactc actcttaact
     8641 ggcccgattt tcttgaacgt cactggcaga aacgcccggt ggtgttaaaa cgcggcttta
     8701 ataattttat tgacccgatc tctccagacg agttggcggg tctggcgatg gaaagcgaag
     8761 ttgacagtcg actggtcagt caccaggatg gcaaatggca ggtcagccac ggcccgttcg
     8821 aaagctacga tcatctcggt gaaaccaact ggtcattact ggtacaggca gtgaaccact
     8881 ggcatgagcc gaccgccgcg ctgatgcgac cgttccgtga actaccggac tggcgtattg
     8941 atgatctgat gatttctttt tctgtacctg gcggcggcgt cggcccgcat ctcgatcagt
     9001 acgacgtgtt tatcattcag ggtaccggac gtcgtcgctg gcgagtgggc gaaaagctgc
     9061 aaatgaaaca gcactgccca catccggatc tgttacaggt cgatccgttc gaagccatca
     9121 tcgatgaaga gctggagcct ggtgatattc tttatattcc gccaggattc ccgcatgaag
     9181 gctacgcgct ggaaaatgcg atgaactatt ccgtgggctt tcgcgcgcca aatacgcggg
     9241 aactgattag tggatttgcc gattatgtgc tgcaacgtga actgggcggc aactactaca
     9301 gcgatcctga tgttccacct cgcgctcatc ctgcggatgt tctgcctcaa gagatggata
     9361 aactgcgtga gatgatgctc gaattgatca accagccgga acactttaag caatggtttg
     9421 gcgagtttat atcccagtca cgtcatgaac tggatatcgc gccgccagaa ccgccttatc
     9481 agccagatga aatctacgat gcgctgaaac aaggtgatgt gctggtgcgc ctgggtggtc
     9541 tgcgcgtatt gcgcattggc gacgacgtgt atgccaatgg tgagaagatc gattccccgc
     9601 accgtccggc actggatgca ctcgccagca acattgcgct gactgcggag aattttggcg
     9661 atgcgctgga agatccgtca ttcctcgcga tgctcgcggc gctggtcaat agcgggtatt
     9721 ggttcttcga agggtaagtt tggatttaag ccggatgcgg catcacatgc cgcatccctt
     9781 ttcgcttact tccgttgcgc cgttaactcg gcaatacgga cgatcacctg caccgctttt
     9841 tccatacctt ccagagtcac aaactcatgc ttaccatgat agttgtaacc gccagtgaac
     9901 aagttcgggc acggtaatcc cataaacgac aactgcgcgc cgtcggtacc accgcggatc
     9961 ggtttcagtt ccggttcaat atcgcagtca cgcatcgcct gctgggcgat atcgagaata
    10021 tgcggatgct caaccacttt ctctcgcata ttgtagtaac tgtcttcaat caccagttca
    10081 atgtagcaat caggatgtaa ccctttgccc acttttttgg cgatctccat cattttacgt
    10141 ttacgcgctt caaactgttt acggtcgaaa tcacggatga tgtagtgcat atcggcccgt
    10201 tcaacggtgc ctttcatgct cgccaggtga tagaaacctt catagccttc tgtcatttcc
    10261 gggctttcat ccgccggaac ttccgcatga atacgtgccg ccagcgacag cgcatttacc
    10321 atcactcctt tcgccgtgcc cggatgaaca ttgttaccga caattttgat attgaccgat
    10381 gcggcgttga agttttcaaa ctccagttcg cctacgccac caccgtcaac agtgtaagcc
    10441 caacgggcat cgaaggcatc aacatcaaaa tgtttcgccc ctttgcccac ttcttcatcc
    10501 ggggtaaagg cgacgcgaat atcaccatgc ggaatgtttt tctgttgcaa taccgccagc
    10561 gcggtcatga tttctgcaat acctgctttg tcatcggcac ctaacaaggt tttaccatcg
    10621 gtggtaatca acgtctgacc cagtagctga tgcagcaccg ggaacataac cggtgataaa
    10681 acttcatcgc cgatacccag cgcaatatcg ccaccgcgat agttttcaac aatttgcgga
    10741 ttaacatttt tgccgctgca atccggtgag gtatccacat gagaaataaa gccaatcgcc
    10801 gggatatcgc cagggacgtt agccggtaac gtcgccatca aagtgccctt ctcacttaag
    10861 gtcacattga taagccccat ttcttcgagc tgctctttca gcagatgcaa taacttccat
    10921 tggccttccg tgctgggaac ctgtctcacc cctgcttttg attgggtatc cagagatacg
    10981 tagttcaaaa atcgctcaag tagtttatcc atgtagtcac cctcactttt tgtgacaaca
    11041 ttattaagaa gcaagaaaag acaaatattg cgtcaggtca cttttacccc tgcaagcggg
    11101 aatatttatc agcattacct taatgaatat aaagctaagc cagtaattca caacaaagat
    11161 tggcgattca agcggtttgc cgtagaatta ccgccctcat taagagtcac caaggtggtt
    11221 aaccacaaac cccgcatcgg taagccatcc gttgcgttta catgggacag agtaaaaaat
    11281 tgaataaaca accgagttcg ctttcaccgc tggtgcaatt ggcgggaatt cgcaaatgct
    11341 ttgatggtaa agaggtcatt ccccagctgg atctgactat caacaatggc gagttcctca
    11401 cgctgcttgg cccttctggc tgcggtaaaa caaccgttct tcgcctgatt gcaggtctgg
    11461 aaactgttga ttccggacgc atcatgctgg ataacgagga catcacccac gttccggcgg
    11521 aaaaccgcta tgtgaacact gttttccaaa gctacgcact tttcccccac atgaccgtgt
    11581 tcgaaaatgt ggcctttggg ttgcgcatgc aaaaaacccc cgctgctgaa attacgcccc
    11641 gcgtgatgga agccctgcgg atggtgcaac tggaaacctt cgctcaacgt aaaccgcatc
    11701 agctctctgg tggtcaacag caacgcgtcg ccattgctcg cgcggtggtt aacaagcctc
    11761 gtctgttgtt gctggatgag tcgctctcag cgctggatta caaactgcgt aagcaaatgc
    11821 agaacgagct gaaagcgtta cagcgtaagc ttggtattac cttcgtcttt gtcactcacg
    11881 atcaggaaga agcgctcacc atgtcagaca ggattgtggt gatgcgcgag ggtcgtattg
    11941 agcaagatgg tacaccgcgc gaaatttacg aagagccgaa aaacctgttt gttgccggct
    12001 tcattggcga aatcaatatg tttaacgcca ctgtcatcga acgtctcgac gagcagcgcg
    12061 tacgcgccaa cgttgaaggc cgcgaatgta atatctacgt taacttcgcc gttgaaccgg
    12121 ggcaaaaact gcatgttctg ctgcgcccgg aagacttacg tgttgaagag attaacgacg
    12181 acaaccacgc tgaagggctg attggttacg ttcgtgagcg taactacaaa ggcatgacgc
    12241 tggagtcggt tgttgaactg gaaaatggca agatggtgat ggtcagcgaa ttcttcaatg
    12301 aagacgaccc tgactttgac cactctctcg accaaaaaat ggccattaat tgggtagaaa
    12361 gctgggaggt cgtactggct gatgaagaac acaagtaagt tccagaatgt agtgattgtc
    12421 actattgtcg gttggcttgt gttgtttgtc tttctgccca acctgatgat cattggcacc
    12481 agctttttga cccgcgacga cgccagtttc gtcaaaatgg tctttacgct ggataactac
    12541 acgcgtctgc tcgatccgct ctattttgaa gtgctattgc actcgctgaa tatggcgctg
    12601 atcgccaccc tcgcctgcct ggtgctgggc tacccgtttg cctggtttct ggcgaagctg
    12661 ccacacaagg tgcgtccgct gctgctgttt ctgctgattg ttcccttctg gaccaactca
    12721 ctgattcgca tctacgggct gaaaattttc ctcagcacca aaggctatct caacgagttt
    12781 ttgctctggc tgggcgttat cgacacacca attcgtatca tgttcacgcc cagtgcggtg
    12841 attatcggtc tggtttacat tctgctaccg tttatggtga tgccgctgta ctccagtatc
    12901 gaaaaactgg ataagccgtt gcttgaggcg gcgcgcgatc tcggtgccag caagttacag
    12961 acttttatcc gtatcattat tccactgacg atgccgggaa ttattgccgg atgtctgctg
    13021 gtgatgctgc cagcgatggg cctgttctat gtatccgacc tgatgggcgg tgcgaaaaac
    13081 ctgctgatcg gtaacgtcat caaggtccag ttcctcaata ttcgtgactg gccgtttggt
    13141 gcagccacca gcattacgct gactatcgta atgggcctga tgttgctggt ttactggcgc
    13201 gcttctcgtc tgctgaataa aaaggtggaa ctcgaatgat cggtcgactg cttcgcggcg
    13261 gttttatgac cgctatctac gcgtacctgt atatcccaat cattattttg attgtgaact
    13321 cctttaacag ctcgcgcttt ggcatcaact ggcagggttt taccaccaaa tggtatagcc
    13381 tgctgatgaa caacgacagc ctgttacagg cagcgcagca ttcactaaca atggcggtgt
    13441 tttcggcgac atttgctacg cttatcggtt cactgacggc agttgcgctg taccgttatc
    13501 gctttcgtgg taagccgttc gttagcggaa tgctgtttgt ggtgatgatg tcaccagata
    13561 tcgtgatggc gatttctctg ctggtgctgt ttatgctgct gggtattcag cttggcttct
    13621 ggtcgctgct gttctcgcat atcaccttct gcctgccgtt tgtggtggtg acggtgtatt
    13681 cgcgcctgaa aggttttgac gtgcggatgc tggaagcggc gaaagatctc ggtgccagtg
    13741 aatttaccat tctgcgaaaa atcattctgc cactggcaat gccagcggtg gcggcgggct
    13801 gggtgttaag ctttaccctg tcgatggacg atgtggtagt ttcttcgttc gtcaccggac
    13861 caagttatga aattctgccg ttaaaaattt attcgatggt caaagtcggc gtatcgccgg
    13921 aagttaacgc gctggcaacc atattactgg tgctgtcgct ggtgatggtt attgccagcc
    13981 agcttattgc tcgtgataaa acgaaaggta acacagggga cgttaaatga aaaaatggtc
    14041 acgccacctg ctcgcggcgg gtgctctggc actgggcatg agcgccgctc acgccgatga
    14101 caacaacacg ctgtatttct acaactggac cgagtacgtg ccgccaggac tgcttgaaca
    14161 gttcaccaaa gaaaccggta ttaaggctat ctattcgact tacgagtcga acgaaaccat
    14221 gtacgccaag ctgaaaacgt acaaagacgg tgcctatgac ctggtggttc cttcaaccta
    14281 ttacgtcgat aaaatgcgta aagaagggat gatccagaag atcgacaagt cgaagttaac
    14341 gaacttcagc aatctcgatc cagacatgct caacaagccg tttgacccga ataacgacta
    14401 ctccattccg tatatctggg gtgcgacggc gattggcgtt aacggtgatg cggtggatcc
    14461 taaatctgtc accagttggg cagatctgtg gaagccagag tacaaaggta gcctgctgct
    14521 gaccgacgat gcccgtgaag tgttccagat ggcgctgcgc aaactgggct actccggtaa
    14581 caccaccgat ccgaaagaga ttgaagctgc atataacgag ctgaaaaaac tgatgccaaa
    14641 cgtcgcagcg tttaactccg ataacccggc taacccgtac atggaaggcg aagttaacct
    14701 cggcatgatc tggaacggct ctgctttcgt tgcacgccag gcgggtacgc caattgacgt
    14761 ggtgtggccg aaagaaggcg gcattttctg gatggacagc ctggcgatcc cggcaaatgc
    14821 caaaaacaaa gaaggcgcgc tgaaattgat caacttcctg ctgcgcccgg atgtggcaaa
    14881 acaggttgcc gaaactatcg gttatccaac gccaaacctt gcggcgcgta agctgttaag
    14941 cccggaagtg gcgaacgata aaacgctcta cccggatgct gaaaccatta aaaatggtga
    15001 atggcagaat gacgttggcg cagccagcag catttatgaa gagtattatc agaagctgaa
    15061 agcaggacgt taattcagca aacgggcggt ggtattaccg cccgtttttt agttagcaag
    15121 gatgtggcta atgagtcagg acagtaaagt tttatcccgc tctttcgtcg gtattgtggt
    15181 ccccttgtag gcctgataag cgtagcgcat caggcaatgc ttcctgcttt ataatccctt
    15241 cagcaacttt tcaacaaact caggcaccac ctggcttgcc gggccgtaat atttctcggc
    15301 aaattcatta ccgacctgac ttggttcgag attcagttcc acggtgtgcg cgccatgcag
    15361 tttcgcttcg tgaacaaacc cagccgccgg ataaacatgc ccggaagtac caatggcaat
    15421 gaaaatatca gccatcgaca acgccatata aatttcatcc atgccgagtg gcatttcgcc
    15481 aaaccaaacg acgtgcgggc gcaagggtgc cgggaactgg caacaatggc atttatcttc
    15541 tggcgtaacg tctccggtcc agtcgagaac ctgaccactt tgcgaacaac gaactttcag
    15601 caattcccca tgcatatgaa tgacattggt attacctgcg cgttcatgca ggttgtctat
    15661 attctgcgtt accagcaaaa agcgatcgcc gagggcgtct tgcagtttag ccagcgcaag
    15721 atgcgcggcg ttaggctgaa tttctggctg ctgcagctgt cgacgacggg cgttataaaa
    15781 cgcttgcacc agttcaggat cgcgatcgaa accttccggc gttgccacat cttcaacccg
    15841 atgttcttcc cacaggccat ctgcggcgcg aaaggtacga atacctgatt ccgcagaaat
    15901 tcctgcccct gtcagtacga gtactcttgg tttttccatt gcttccggca ccactttatc
    15961 tctgaaaaaa atacgctgac gcaaacgctc gcgcaggcgg cgtttatttt tacgaaaacg
    16021 acttaaccga tgaccccgac gcgacagcat agcaacctct gttgtttaat cggttagatg
    16081 taggaaggcc gcaccacgca ttcctcccgc atcaccgtgg cgcgcgcgtt caatgcgcgg
    16141 aacacgagct acaggtaaga gatgacgagg cagcctgtcc gccagttgcg ttgtgattgc
    16201 cgggaaattc gataagccac caccaatgac gaccaggtca gagtcaacga tggtcaggat
    16261 atttcccaga caaaccgcta ataaatccag ataacgctca acgtgcgccc ttgcctgctc
    16321 atcgccttga tcataaagcg caataatttc gggagcctgc aacggttgat gataatagtg
    16381 ttgccagagc cacgcaaagc cccgacctga tagataattt tcgatacaac caatttgacc
    16441 acagccgcag cggcgtaacg ggaaatccag ccccatcatg gttaacgcat caaccggcag
    16501 acgcatatgg ccaaactcgc cggtaatgta gcttttaccg gtaatcggtt taccgttgaa
    16561 aatcagcccg ccgccaacgc cggtgccgag aattaacccc atcaccagtg gatattgcgt
    16621 aaattcatca tcccaggctt ctgaaagggc aaaacagttg gcatcgttat caaggcgtac
    16681 atcgcgatca agacgtgcgc tcaggtcggc acgcagcggt ttaccgctgg cagcggggac
    16741 attggcggca tacagcgtac catcttctgt ttccggcatt cccggaatac cgatgccaac
    16801 agaaccttta cagccaaaac gttggtcagc ttcggctacc agctcgcata ctgcatctaa
    16861 aaatgcgtca tagctgtcac gcggtgtcgg cacccgcttt tcccactgca actgccgacc
    16921 gctatcaaac acgccaagcg caatttttgt tccaccaata tcaaacccgt aatacattgc
    16981 cgctccttgt tttaatgtac tgcctttact ggccgctaag gactcgcgca gggtcaatat
    17041 tactggcgcg ccgcgccgga taccaacttg ccaaaagact cagcaacaat gctgtgacca
    17101 gtacgtagaa gacgtccagc cagtgcaatt ccgatggcag gaagtcaata aaatagatat
    17161 cgctggagag gaactgatga ccgatcagct tttcaatccg ctcaataatc ggggtaagtt
    17221 gcagtgaaac aactacgccg ataatcacac cacacaggct accgaacagc cctgccagca
    17281 atccatacca gacaaagatg gcgcgaatta aaccatcttt cgcccccagc gttcttaata
    17341 ctgcgatatc gccactcttg tctttcaccg ccatcactaa ggtggagacg atgttgaaac
    17401 aggccacgcc aatcaccagt accatcgcca gatacataat ggcgcggatc atctggatat
    17461 cgcgatacat atagccgtaa gtaccaatcc aacttttaat ataaacatag ctgttggtca
    17521 cttcacccgc atcgcgtacc agcttattgg cgttgaaaac atccgtcatt ttaagggcaa
    17581 tacctgacac gctggaaccc atatcaagat attgttgggc atccgccagc gggatcatgg
    17641 caaaactgtg atcgagttga ccactcaact gcaaaatacc ggcaatgtgc aaacgcacac
    17701 gttttggctg catcagttta tgctcgggat tcgagttggg aatcatgatc gacacccaat
    17761 cgccctgctt cactttcagc gcatccgcca cgcctttgcc gatgataatt tgctgttcgc
    17821 ccgctttaaa attgcgccag gcatccccct gaacaaacga gggtaatgcg ctcagacgct
    17881 gttcctgttg cgggttaacg cccttcacct gaattgcgcg cagattggta ccactttcta
    17941 ccagtccggt gaaattgata tacggcgcag cggcggcaat acccggcact ttttgcacgt
    18001 tatccagtgc ttcctgccag ttagtccatg gctgatttac cgcctcaatt tcaccgtgcg
    18061 gtaccacagc aagaatgcgg ttattcagtt cgcgttcaaa gccgttcatc gcgcttaagc
    18121 cgacgatcaa taccgccacg ccgagggcaa tgccaatggt agaaatgaca gagattagcg
    18181 acaccatgcc gccgcgtcgc cgtccgcgac taaaacgcag gccaatcaat aacgataaag
    18241 gcatcgccat tactccgccc ccatcaggct cagttccgcc gtcagacgtc catcacgcat
    18301 ttccagttgg cggctcatac gtttcgccag ttgcaggtcg tgagtaacca ccaggaaggc
    18361 ggtgccctgc aagcgattca attccccaag caactggaag atgctgtcgg cgttacgcgc
    18421 atcgaggtta ccggtaggct catccgcaag taccagacgc ggattattga ccagcgcacg
    18481 ggcaatcgcc acacgctggc gttcgccgcc agaaagttca gatgggcggt gattcgcacg
    18541 atgctccagc cccaccgctt ttaacatctc cagcgcacgg ctgttgattt cagcgggctt
    18601 tttcttgcca atcagcagtg gcatagccac gttttccagg gcagtaaaat ccggcagcag
    18661 gtggtgaaac tgataaataa agcccagctt ctggttgcgc agttctgctt tcgccgccga
    18721 agacagtttg ctcatcggtt gaccgttaaa gatcacatcg ccggaggttg gtgtatccag
    18781 cccgcccagc aggtgcagca aggtactttt accggaacca gagctaccga cgatcgccat
    18841 catttcacct tcgccgacgc tgaaactgac attgtgcagt acatcggttt gcacactgcc
    18901 ttcctgatag cgtttgcaca ggttgtcgca ttgcaacagg atcttattca taacgtaaag
    18961 cctcagcggg ttgagtggcg gcagcgcgcc atgaagggta aagcgtagac agcagcgcga
    19021 tagccatcgc caccagcgca ataacaatga cctgtaaagg ttcgatagcg accggcagcg
    19081 ccgcgccatc aagcaggacg ccgattatcg gcatcagatt atttaactgg ctggcaagca
    19141 gtgcgccaag cgccgctccg aggatcgcac cgataatccc ggcgctggcc ccttgcacca
    19201 taaagaccat catgatttgt cgcggagtta agccttgcgt ttgcaggatc gcgacttcgc
    19261 cctgcttctc cattaccatc agccctagtg aggtaataat gttaaacgcc gcaacggcga
    19321 caatcaggct cagcagcaag cccatcatat ttttttccat gcgtacggcc tggaacagct
    19381 cgcctttacg gtcacgccag tcctgccatt tgctgccttc aggcagtttt tgctgactta
    19441 aagagtcaac tttcagcggc tcatccagcc acaaacgcca gccggtaata ttgcctgccg
    19501 gataacgcat cagacgcgag gcatcctcaa tattcaccag catttcatag ccatcgactt
    19561 cactgttggc ggcgaaattg ccaatcacat tgaacaggcg ctggcttgga atacgcccca
    19621 tcggcgtgaa ctggctggca gatggtacca tcacgcggat ttgatcaccg cgattaacgc
    19681 ctagctgtga ggcaagttgt tcgccgagga tgacattata tttccccggc tcgaggtcag
    19741 tttgtttcac attgaccaga tacggcgtaa gtggatcttt ttgcgccggg tcgataccga
    19801 gcatcacccc gaccgccacg ctgcgcgcgc tttgcagtac cacatcaccg gtagtaatag
    19861 gtgcgacgcg attaacgccg tccagtttga ctgccgtttc cgggagttgc tgcgggttaa
    19921 gagagccatg ctcagaagag agaattgcct gtggcatcag gccaaggatg ttgttttgca
    19981 gctcgcgctc aaagccgttc atcactgaca atactgtgac cagcgccatc accccgaggg
    20041 taatgccgat ggtagaaagc caggagacga aacgaccgaa gcgatccgct gcacgcccac
    20101 gcatgtaacg caggccaata aatagagcga caggttggta catgaaatcc gtctggttgc
    20161 tgtagcaaag acacgagtat ataagcgaaa gcaagatggg taaacgtctg aactgtaagt
    20221 gatcgtcagg gctaatctga aaagcccccg taaatcaatc ggctaaattg ccatgtttta
    20281 tcatcaatct tgccgcctga tcttaatctg aaaaatcatc caatacagac agatacccat
    20341 tacacctcgg ctcataaccc gcaggatcaa ccgcatgtaa attaaaaaag agcgtgttgt
    20401 cctgatgaaa caaaaagagc tatggattaa ccagatcaaa gggttatgta tttgtctggt
    20461 ggtgatttat cactcggtca ttacctttta tccgcatatg accacttttc agcatccgtt
    20521 atcggaagtc ctgagtaaat gctggatcta tttcaatctt taccttgcac cctttcgtat
    20581 gccggttttt ttctttatat ccggctattt gattcgccgc tatatcgaca gcgtaccgtg
    20641 gggaaattgt ctcgataaac ggatctggaa catcttctgg gtgctggcgc tttggggcgt
    20701 ggtgcagtgg ctggcgttaa gtgcgctgaa ccagtggctg gcacctgagc gcgatttaag
    20761 caatgcctcc aatgccgctt atgccgattc caccggtgag ttcctgcacg ggatgatcac
    20821 cgccagcacc agcttgtggt atctgtatgc gttaattgtc tatttcgtgg tatgtaaaat
    20881 ttttagccgt ctggcgctgc cactattcgc cttgtttgta ctgctgagtg tggcggttaa
    20941 tttcgttccc acgccgtggt ggggaatgaa cagtgtgatc cgcaatttgc cttattacag
    21001 ccttggcgca tggtttggcg caacaataat gacctgtgtt aaagaggtgc cgttgcgccg
    21061 ccatctgctg atggcttctt tgcttaccgt tctggcggtc ggtgcctggt tgtttactat
    21121 ctcgctgctg ttgtcgctgg tatcgattgt ggtgatcatg aagctgtttt atcagtacga
    21181 gcaacgattc ggtatgcgct ccaccagcct gctgaatgtg attggctcca acaccattgc
    21241 tatctacacc acccatcgca ttctggttga aatattcagc ttaactctgc ttgcgcaaat
    21301 gaacgcagca cgctggtcgc cgcaagtcga actgacactc ctgctggttt atccctttgt
    21361 cagtttgttc atctgtactg ttgcgggctt gctggtaaga aaactttcac agcgtgcatt
    21421 cagcgatctg ttgttctccc cgccttcact gcccgctgcc gtcagttact ctcgttaaac
    21481 catcgcccca gccggggcga ttcaatttgc taaaccatgt cgattacgga taatgaacag
    21541 cattgcttat cagagatatg cccccatatg ttgaggcata tcctaacgag aatctgacaa
    21601 ccgttatgcc tgaacaatat cgttatacac tgcccgtcaa agcgggtgag cagcgtctgc
    21661 tgggcgagtt aaccggcgca gcctgtgcaa cgctggtagc ggaaattgcc gaacgtcacg
    21721 ccggtccggt ggtactcatt gcaccagata tgcaaaatgc tctgcgtttg catgatgaaa
    21781 tcagccagtt caccgatcag atggtgatga atctggcgga ctgggaaact cttccctacg
    21841 acagtttttc gcctcatcag gacattatct cctcgcgcct ttccaccctt taccagctac
    21901 cgacgatgca gcgtggcgta ctgattgttc cggtgaatac gcttatgcag cgcgtttgcc
    21961 cacacagttt tctccacggt catgcgctgg tgatgaaaaa aggtcagcgc ctgtcacgag
    22021 atgcattacg aacccaactg gacagcgccg gttatcgcca tgttgaccag gtgatggagc
    22081 acggcgaata cgccacgcgc ggcgcgttgc tggatctctt cccgatgggg agtgagctgc
    22141 cttatcgtct tgatttcttt gatgatgaaa tcgacagcct gcgggtgttt gacgtcgaca
    22201 gccagcgcac gctggaggaa gtagaagcga tcaatctgct gcccgcgcac gaatttccga
    22261 ccgataaagc ggcaattgaa ctgttccgca gccagtggcg cgataccttc gaagtgaagc
    22321 gcgatccgga acatatttac cagcaagtga gtaaaggcac attacctgcc gggatcgagt
    22381 actggcagcc attgttcttc agcgaaccac tgccgccgct gttcagttat ttccctgcca
    22441 ataccttgct cgtgaatact ggcgatctgg aaaccagtgc cgaacgtttc caggctgaca
    22501 cgctggcgcg ttttgagaat cgcggcgtcg atccgatgcg cccgctgttg ccaccacaat
    22561 cgctctggct gcgggtggac gagctcttct cagagctgaa aaactggccc cgggtgcagc
    22621 taaaaactga acatttaccg acaaaagccg cgaatgccaa tttaggtttc cagaaactgc
    22681 cagacctggc cgttcaggca caacaaaaag cgccgctgga tgcgctgcgt aagttcctcg
    22741 agactttcga cggtccggtg gtgttctcgg tagaaagtga aggtcgccgt gaagcgctgg
    22801 gtgaactgct cgcacgaatt aaaattgctc cgcaacgcat tatgcgtctt gatgaagcca
    22861 gcgaccgtgg gcgttatctg atgattggcg ctgccgaaca tggttttgtc gataaggtgc
    22921 gtaatctggc gctgatctgc gaaagcgatc tgctcggtga acgtgttgcc cgccgtcgtc
    22981 aggattctcg ccgcaccatc aaccccgata cactgatccg taaccttgcg gagctgcata
    23041 ttggtcagcc ggtggtccat ctggagcacg gtgtcgggcg ctatgccgga atgaccacgc
    23101 tcgaagcggg cggcattact ggcgagtatt tgatgctcac ctatgccaac gacgccaaac
    23161 tgtatgttcc ggtgtcgtca ctgcatctga ttagccgtta cgcaggtggc gcggaagaaa
    23221 acgccccgct gcataaactt ggcggcgatg cgtggtcacg cgcgcggcag aaagcggcgg
    23281 aaaaagtgcg tgatgtggcg gcggaattgc tggatatcta cgcgcaacgc gccgccaaag
    23341 agggcttcgc gtttaaacac gatcgtgagc agtatcagtt gttctgcgac agcttcccgt
    23401 ttgaaaccac gccggatcag gcgcaggcca ttaatgcggt acttagcgac atgtgtcagc
    23461 cgctggcaat ggatcgtctg gtgtgcggcg atgttggctt tggtaaaaca gaagtggcga
    23521 tgcgcgccgc tttcctggca gtagataacc acaagcaggt ggcggtgttg gtgcctacca
    23581 cccttctcgc gcagcagcat tacgacaact tccgcgaccg tttcgccaac tggccggtac
    23641 gtatcgaaat gatctcccgt ttccgcagcg ccaaagagca gacgcaaatc cttgcggaag
    23701 tggcggaagg gaaaatcgat attctgatcg gtacgcacaa actgctgcaa agtgacgtca
    23761 agtttaaaga tttaggcctg ctgattgtcg atgaagaaca ccgcttcggg gtgcgtcata
    23821 aagagcgcat taaagcgatg cgcgcgaacg tggatattct gacgcttact gcaacgccga
    23881 tcccacgcac gctgaatatg gcaatgagcg gaatgcgtga cctgtcgatt atcgccacgc
    23941 cgcccgcccg tcgtctggca gttaaaacct ttgtccgtga gtatgacagc ctggtggtcc
    24001 gggaggcgat cctgcgtgaa attttgcgcg gggggcaggt ttattatctc tacaatgatg
    24061 tggaaaacat ccagaaagct gccgaacggc tggcagaact ggtgccagaa gcgcggatcg
    24121 ccatcggtca cgggcagatg cgcgagcgcg aactggaacg ggtgatgaat gatttccatc
    24181 atcaacgttt caacgtgctg gtttgtacaa ccattatcga aaccgggatc gacatcccga
    24241 cagccaacac cattatcatt gaacgcgcgg atcacttcgg tctggcgcag ctgcaccagt
    24301 tacgcggtcg cgtcggacgt tcgcatcatc aggcatatgc atggttgctg acaccgcatc
    24361 caaaagcgat gactaccgat gcacaaaaac gtcttgaagc gattgcctcg ctggaagatc
    24421 tcggggcagg ttttgcgctg gcaacgcacg atctggagat tcgcggcgcg ggtgaactgc
    24481 ttggcgaaga acaaagcggc tcaatggaaa ccatcggttt ctcgctgtat atggagttgc
    24541 tggaaaacgc cgtcgatgca ctgaaagccg gacgcgagcc gtcgctggaa gatctcacca
    24601 gccagcaaac agaagtcgag ctgcggatgc cgtcgctatt gccagatgat ttcattcctg
    24661 acgtgaatac gcgtttgtcg ttctataaac gtattgccag cgccaaaacg gaaaacgaac
    24721 tggaagagat caaagtcgag cttatcgatc gcttcggcct gctgccagat ccggcgcgta
    24781 ccctgctgga tattgcccgt ctgcgccagc aagcgcagaa actggggatc aggaagctgg
    24841 aaggtaatga gaaaggcggg gtgatcgaat ttgccgagaa gaatcacgtt aatccggcct
    24901 ggttgattgg tttgctgcaa aaacagccgc agcattaccg ccttgatggt ccgacgcgcc
    24961 tgaaatttat tcaggatttg agtgagcgga aaacgcgtat cgaatgggta cgccagttta
    25021 tgcgtgaact ggaagagaac gcgatcgctt aatggtaaat cgggggcgtt tctgcgcccc
    25081 catctgcaac atttacaaat tctttgcact tccctgcact atccgacact gtcaccatcc
    25141 ataattcagg cttatctgtt tattacaata accttatatt tattattgat ttttggtgat
    25201 gatcaaaacg cgtttttctc gctggctaac gttttttacg ttcgccgctg ccgtggcgct
    25261 accggcaaaa gccaacacct agccgctgcc gccagcgagc agtcgtctgg ttggcgaaaa
    25321 caaatttcat gtggtggaaa atgacggtgg ttctctggaa gccatcgcca aaaaatataa
    25381 cgtcggcttt ctcgctctgt tacaggctaa ccccggcgtt gatccttacg taccgcgcgc
    25441 gggcagcgtg ttaacgatcc cgttgcaaac cctacttcca gacgcgccgc gcgaaggcat
    25501 tgtgatcaac attgcggagc tgcgtctcta ttactacccg ccgggtaaaa attcggtaac
    25561 cgtgtatcca ataggtattg gtcagttagg tggtgacacg ctgacaccga caatggtgac
    25621 caccgtttca gacaaacgtg caaacccaac ctggacgcca acggcaaaca tccgcgcccg
    25681 ttataaagca cagggaattg agttgcctac ggtagtgccg gctggaccgg ataacccaat
    25741 gggccatcat gcgattcgtc tggcggccta tggcggcgtt tatttgcttc acggtacgaa
    25801 cgccgatttc ggcattggca tgcgggtaag ttctggctgt attcgtctgc gggatgacga
    25861 tatcaaaaca ctctttagcc aggtcacccc aggcaccaaa gtgaatatca tcaacactcc
    25921 gataaaagtc tctgccgaac caaacggtgc gcgtctggtt gaagtacatc agccgctgtc
    25981 agagaagatt gatgacgatc cgcagctgct gccaattacg ctgaatagcg caatgcaatc
    26041 atttaaagat gcagcacaaa ctgacgctga agtgatgcaa catgtgatgg atgtccgttc
    26101 cgggatgccg gtggatgtcc gccgtcatca agtgagccca caaactctgt aaatcctgga
    26161 taaaaaaaat ccccgcgaca cggcatgtcg cggggaaaaa atcagcagta gcaggcatta
    26221 atgagggtta atgcttattt ataaattact gctgttccat ggagggtatt cggaccggtt
    26281 acagaagtaa tacggaaaga ttttgcgccc atctcatccg ctttttgcgc cagctgctct
    26341 tccagcgatc ccagatttgt ccccgcgtta gcactgatag taccgacttt ttgttggcct
    26401 tctggcgttg actgaacttc gacagccgca aagttggcaa atgacatgga gcttaaaatc
    26461 gccgcagcga tgagggtttt tacgtttttc ataatagtgg ccttatgcag atgaatgacg
    26521 tcgcaagaaa ttacttaacg atcgataact aaatgata
//
LOCUS       DADMKF010000048        25908 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.049, whole genome shotgun sequence.
ACCESSION   DADMKF010000048 DADMKF010000000
VERSION     DADMKF010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25908
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.049"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            16..486
                     /locus_tag="JNC24_001803"
     CDS             16..486
                     /locus_tag="JNC24_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HAY9158559.1"
                     /translation="MVDQNLPWSASLKVGDKKLPVVVVRVGEASVDVLVGQQTWTLTH
                     KWFESVWTGDYLLLWKMSPEGESTITRDSSEEEILWLETMLNRALHISTEPSAEWRPL
                     LVEKIKQFQKSHHLKTDGVVGFSTLVHLWQVAGESAYLYRDEANISPETTVKGK"
     gene            488..907
                     /gene="gspB"
                     /locus_tag="JNC24_001804"
     CDS             488..907
                     /gene="gspB"
                     /locus_tag="JNC24_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative general secretion pathway protein GspB"
                     /protein_id="HAY9158560.1"
                     /translation="MFEFYIAAREQKETGHPGIFSRQKHSTIIYVICLLLICLWFAGM
                     VLVGGYARQLWALWIVKAEVTVEAETPAFKQSTQHYFFKKQPLPVVESVEEEDDPDVA
                     VENAPSSSEDEENTVKESDEKAGLRERVKNALNELER"
     gene            1145..1456
                     /gene="rpsJ"
                     /locus_tag="JNC24_001805"
     CDS             1145..1456
                     /gene="rpsJ"
                     /locus_tag="JNC24_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HAY9158561.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            1489..2118
                     /gene="rplC"
                     /locus_tag="JNC24_001806"
     CDS             1489..2118
                     /gene="rplC"
                     /locus_tag="JNC24_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HAY9158562.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     EVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            2129..2734
                     /gene="rplD"
                     /locus_tag="JNC24_001807"
     CDS             2129..2734
                     /gene="rplD"
                     /locus_tag="JNC24_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HAY9158563.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            2731..3033
                     /gene="rplW"
                     /locus_tag="JNC24_001808"
     CDS             2731..3033
                     /gene="rplW"
                     /locus_tag="JNC24_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HAY9158564.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            3051..3872
                     /gene="rplB"
                     /locus_tag="JNC24_001809"
     CDS             3051..3872
                     /gene="rplB"
                     /locus_tag="JNC24_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HAY9158565.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            3889..4167
                     /gene="rpsS"
                     /locus_tag="JNC24_001810"
     CDS             3889..4167
                     /gene="rpsS"
                     /locus_tag="JNC24_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HAY9158566.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            4182..4514
                     /gene="rplV"
                     /locus_tag="JNC24_001811"
     CDS             4182..4514
                     /gene="rplV"
                     /locus_tag="JNC24_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HAY9158567.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VLVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            4532..5233
                     /gene="rpsC"
                     /locus_tag="JNC24_001812"
     CDS             4532..5233
                     /gene="rpsC"
                     /locus_tag="JNC24_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HAY9158568.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            5246..5656
                     /gene="rplP"
                     /locus_tag="JNC24_001813"
     CDS             5246..5656
                     /gene="rplP"
                     /locus_tag="JNC24_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HAY9158569.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            5656..5847
                     /gene="rpmC"
                     /locus_tag="JNC24_001814"
     CDS             5656..5847
                     /gene="rpmC"
                     /locus_tag="JNC24_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HAY9158570.1"
                     /translation="MKAKELREKSVEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            5847..6101
                     /gene="rpsQ"
                     /locus_tag="JNC24_001815"
     CDS             5847..6101
                     /gene="rpsQ"
                     /locus_tag="JNC24_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HAY9158571.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            6266..6637
                     /gene="rplN"
                     /locus_tag="JNC24_001816"
     CDS             6266..6637
                     /gene="rplN"
                     /locus_tag="JNC24_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013975775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HAY9158572.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            6648..6962
                     /gene="rplX"
                     /locus_tag="JNC24_001817"
     CDS             6648..6962
                     /gene="rplX"
                     /locus_tag="JNC24_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HAY9158573.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            6977..7516
                     /gene="rplE"
                     /locus_tag="JNC24_001818"
     CDS             6977..7516
                     /gene="rplE"
                     /locus_tag="JNC24_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HAY9158574.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            7531..7836
                     /gene="rpsN"
                     /locus_tag="JNC24_001819"
     CDS             7531..7836
                     /gene="rpsN"
                     /locus_tag="JNC24_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAY9158575.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            7870..8262
                     /gene="rpsH"
                     /locus_tag="JNC24_001820"
     CDS             7870..8262
                     /gene="rpsH"
                     /locus_tag="JNC24_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HAY9158576.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            8275..8808
                     /gene="rplF"
                     /locus_tag="JNC24_001821"
     CDS             8275..8808
                     /gene="rplF"
                     /locus_tag="JNC24_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HAY9158577.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGVDKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            8818..9171
                     /gene="rplR"
                     /locus_tag="JNC24_001822"
     CDS             8818..9171
                     /gene="rplR"
                     /locus_tag="JNC24_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HAY9158578.1"
                     /translation="MDKKFARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            9186..9689
                     /gene="rpsE"
                     /locus_tag="JNC24_001823"
     CDS             9186..9689
                     /gene="rpsE"
                     /locus_tag="JNC24_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HAY9158579.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            9693..9872
                     /gene="rpmD"
                     /locus_tag="JNC24_001824"
     CDS             9693..9872
                     /gene="rpmD"
                     /locus_tag="JNC24_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HAY9158580.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            9876..10310
                     /gene="rplO"
                     /locus_tag="JNC24_001825"
     CDS             9876..10310
                     /gene="rplO"
                     /locus_tag="JNC24_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HAY9158581.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            10318..11649
                     /gene="secY"
                     /locus_tag="JNC24_001826"
     CDS             10318..11649
                     /gene="secY"
                     /locus_tag="JNC24_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HAY9158582.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFVGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            11681..11797
                     /gene="rpmJ"
                     /locus_tag="JNC24_001827"
     CDS             11681..11797
                     /gene="rpmJ"
                     /locus_tag="JNC24_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HAY9158583.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            11944..12300
                     /gene="rpsM"
                     /locus_tag="JNC24_001828"
     CDS             11944..12300
                     /gene="rpsM"
                     /locus_tag="JNC24_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018356918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HAY9158584.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            12317..12706
                     /gene="rpsK"
                     /locus_tag="JNC24_001829"
     CDS             12317..12706
                     /gene="rpsK"
                     /locus_tag="JNC24_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HAY9158585.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            12740..13360
                     /gene="rpsD"
                     /locus_tag="JNC24_001830"
     CDS             12740..13360
                     /gene="rpsD"
                     /locus_tag="JNC24_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HAY9158586.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            13386..14375
                     /gene="rpoA"
                     /locus_tag="JNC24_001831"
     CDS             13386..14375
                     /gene="rpoA"
                     /locus_tag="JNC24_001831"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HAY9158587.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            14416..14799
                     /gene="rplQ"
                     /locus_tag="JNC24_001832"
     CDS             14416..14799
                     /gene="rplQ"
                     /locus_tag="JNC24_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HAY9158588.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
     gene            14906..15274
                     /locus_tag="JNC24_001833"
     CDS             14906..15274
                     /locus_tag="JNC24_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HAY9158589.1"
                     /translation="MWLLDQWAERHIAEAQAKGEFDNLAGSGEPLILDDDSHVPPELR
                     AGYRLLKNAGCLPPELEQRREAIQLLDILKGIRHDDPQYQEVSRRLSLLELKLRQAGL
                     STDFLRGDYADKLLDKINDN"
     gene            15285..15710
                     /gene="zntR"
                     /locus_tag="JNC24_001834"
     CDS             15285..15710
                     /gene="zntR"
                     /locus_tag="JNC24_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(2+)-responsive transcriptional regulator"
                     /protein_id="HAY9158590.1"
                     /translation="MYRIGELAKMAEVTPDTIRYYEKQQMMEHEVRTEGGFRLYTESD
                     LQRLKFIRHARQLGFSLESIRELLSIRIDPEHHTCQESKGIVQERLQEVEARIAELQS
                     MQRSLQRLNDACCGTAHSSVYCSILEALEQGASGVKSGC"
     gene            15766..15984
                     /locus_tag="JNC24_001835"
     CDS             15766..15984
                     /locus_tag="JNC24_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor A"
                     /protein_id="HAY9158591.1"
                     /translation="MSRYQHTKGQIKDNAIEALLHDPLFRQRVEKNKKGKGSYMRKGK
                     HGNRGNWEASGKKVNHFFTTGLLLSGAC"
     gene            complement(15981..16391)
                     /gene="mscL"
                     /locus_tag="JNC24_001836"
     CDS             complement(15981..16391)
                     /gene="mscL"
                     /locus_tag="JNC24_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HAY9158592.1"
                     /translation="MSIIKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSLVADIIMPP
                     LGLLIGGIDFKQFAVTLRDAQGDIPAVVMHYGVFIQNVFDFLIVAFAIFMAIKLINKL
                     NRKKEEPAAAPAPTKEEVLLAEIRDLLKEQNNRS"
     gene            complement(16521..17897)
                     /gene="trkA"
                     /locus_tag="JNC24_001837"
     CDS             complement(16521..17897)
                     /gene="trkA"
                     /locus_tag="JNC24_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HAY9158593.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(17919..19208)
                     /gene="rsmB"
                     /locus_tag="JNC24_001838"
     CDS             complement(17919..19208)
                     /gene="rsmB"
                     /locus_tag="JNC24_001838"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HAY9158594.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRQQEELLAEFNTSDARYLHPSWLLKRLQKAYPEQ
                     WQSIVEANNQRPPMWLRVNRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRVHDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNLPGAEEGDGFFYAKLIKK"
     gene            complement(19254..20201)
                     /gene="fmt"
                     /locus_tag="JNC24_001839"
     CDS             complement(19254..20201)
                     /gene="fmt"
                     /locus_tag="JNC24_001839"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HAY9158595.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPVKVLAEEKGLPVFQPVSLRPQESQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLA"
     gene            complement(20216..20725)
                     /gene="def"
                     /locus_tag="JNC24_001840"
     CDS             complement(20216..20725)
                     /gene="def"
                     /locus_tag="JNC24_001840"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAY9158596.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEAEGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            20855..21979
                     /gene="dprA"
                     /locus_tag="JNC24_001841"
     CDS             20855..21979
                     /gene="dprA"
                     /locus_tag="JNC24_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HAY9158597.1"
                     /translation="MVDTEIWLRLMSISSLYGDDMVRIAHWLAKQSQIDAVGLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLLVTTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLAASLLEHGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQRREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            21951..22424
                     /locus_tag="JNC24_001842"
     CDS             21951..22424
                     /locus_tag="JNC24_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HAY9158598.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFEREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDNAEFELDDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            22453..22995
                     /locus_tag="JNC24_001843"
     CDS             22453..22995
                     /locus_tag="JNC24_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9158599.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RLLKSSADGHIVKVLEGQVCPACGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            23000..23572
                     /gene="tsaC"
                     /locus_tag="JNC24_001844"
     CDS             23000..23572
                     /gene="tsaC"
                     /locus_tag="JNC24_001844"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HAY9158600.1"
                     /translation="MNNNLQGDAIAAAIDVLNEERVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDAQRETIFSRWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            23577..24395
                     /gene="aroE"
                     /locus_tag="JNC24_001845"
     CDS             23577..24395
                     /gene="aroE"
                     /locus_tag="JNC24_001845"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HAY9158601.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFRAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GVGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKLLQQELSA"
     gene            24392..24649
                     /locus_tag="JNC24_001846"
     CDS             24392..24649
                     /locus_tag="JNC24_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HAY9158602.1"
                     /translation="MNQAIQFPDREEWDENKRCVCFPALVNGMQLTCAISGESLAYRF
                     TGGTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(24625..25179)
                     /locus_tag="JNC24_001847"
     CDS             complement(24625..25179)
                     /locus_tag="JNC24_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HAY9158603.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
     gene            25652..>25908
                     /locus_tag="JNC24_001848"
     rRNA            25652..>25908
                     /locus_tag="JNC24_001848"
                     /product="16S ribosomal RNA"
BASE COUNT         6609 a         6107 c         6792 g         6400 t
ORIGIN      
        1 gcttctttac aaacattggt agatcagaat ttgccctgga gcgcatcgct taaagtcggg
       61 gacaaaaaat tgcctgttgt ggtcgttcgc gtcggtgagg ctagcgttga tgtcctcgta
      121 ggtcagcaaa cgtggacgct gacacataaa tggtttgagt cggtatggac gggtgattat
      181 cttctgttgt ggaaaatgtc gccggaaggg gagagtacca ttacgcgtga tagcagcgag
      241 gaagagattc tctggctgga aacgatgtta aaccgtgcat tgcatatttc gacagaacct
      301 tcggcggagt ggcgtccctt gttggtagaa aaaataaagc aatttcaaaa aagccaccac
      361 ttgaaaactg acggtgttgt tggcttttca acgttagtac atctctggca ggtggcaggg
      421 gagagtgcct acttatatcg ggatgaagca aatatttccc cggaaacaac agtgaagggg
      481 aaatgacatg tttgaattct atatcgccgc gcgtgagcag aaagaaacag gtcaccctgg
      541 tatcttctcc cgacaaaaac acagcaccat aatatatgtc atttgtttat tgctgatttg
      601 cctgtggttt gcaggaatgg tgttggttgg tgggtatgcc aggcagctat gggcgctttg
      661 gatagtaaaa gcagaagtca ctgtagaggc tgaaacgccc gcttttaaac aatcaacaca
      721 acactatttc ttcaaaaaac agccactccc cgtcgtcgaa tccgtcgagg aggaagatga
      781 cccagacgtc gcggttgaga atgctccctc ttcttctgaa gacgaagaaa atactgtaaa
      841 agaaagcgac gagaaagccg gtttaagaga gcgtgtcaaa aatgcgctga acgaacttga
      901 gagataaccc gaaggctgtt tactttacta gcaatacgct tgcgttcggt ggttaagtat
      961 gtataatgcg cgggcttgtc gtagttgaca gcaggttcaa tctgaacctc agttagccga
     1021 atttggctac ctaacaatgc tcccaatcgg ggagctacgt aagaacggtt acactctccc
     1081 atcaatcgta atgggtctga ggagtaatca ttttcgttta taaaataatt ggagctctgg
     1141 tctcatgcag aaccaaagaa tccgtatccg cctgaaagcg tttgatcatc gtctgatcga
     1201 tcaagcaacc gcggaaatcg tcgagactgc caagcgcact ggtgcgcagg tccgtggtcc
     1261 gatcccgctg ccgacacgca aagagcgctt cactgttctg atctccccgc acgtcaacaa
     1321 agacgcgcgc gatcagtacg aaatccgtac tcacttgcgt ctggttgaca tcgttgagcc
     1381 aaccgagaaa accgttgatg ctctgatgcg tctggatctg gctgccggtg tagacgtgca
     1441 gatcagcctg ggttaatcag gtcattgagc gattgagagg ttgaaacaat gattggttta
     1501 gtcggtaaaa aagtgggtat gacccgtatc ttcacagaag acggcgtttc tatcccagta
     1561 accgtaatcg aagttgaagc aaaccgcgtt actcaggtta aagacctggc taacgatggc
     1621 taccgtgcta ttcaggtgac caccggtgct aaaaaagcta accgtgtgac caagcctgaa
     1681 gctggccact tcgctaaagc tggcgtagaa gctggccgtg gtctgtggga attccgcctg
     1741 gctgaaggcg aagagttcac tgtaggtcag agcattagcg ttgaactgtt tgctgaggtt
     1801 aaaaaagttg acgtaactgg cacctctaaa ggtaaaggtt tcgcaggtac cgttaagcgc
     1861 tggaacttcc gtacccagga cgctactcac ggtaactcct tgtctcaccg cgttccgggt
     1921 tctatcggtc agaaccagac tccgggcaaa gtgttcaaag gcaagaaaat ggcaggtcag
     1981 atgggtaacg aacgtgtaac cgttcagagc cttgacgtag tacgcgttga cgctgagcgc
     2041 aacctgctgc tggttaaagg tgctgtcccg ggtgcaaccg gtagcgacct gatcgttaaa
     2101 ccagctgtga aggcgtaagg agatagcaat ggaattagta ttgaaagacg cgcagagcgc
     2161 gctgactgtt tccgaaacta ccttcggtcg tgatttcaac gaagcgctgg ttcaccaggt
     2221 tgttgttgct tatgcagctg gtgctcgtca gggtactcgt gctcagaaga ctcgtgctga
     2281 agtaactggt tccggtaaaa aaccgtggcg ccagaaaggc accggccgtg cgcgttctgg
     2341 ttctatcaag agcccgatct ggcgttctgg tggcgtgacc tttgctgctc gtccgcagga
     2401 ccacagtcaa aaagttaaca agaagatgta ccgcggcgcg ctgaaaagca tcctgtccga
     2461 actggtacgt caggatcgtc tgatcgttgt cgagaagttc tctgtagaag cgccgaaaac
     2521 taagctgctg gcacagaaac tgaaagacat ggctctggaa gatgtgctga tcatcaccgg
     2581 tgagctggac gaaaacctgt tcctggctgc gcgcaacctg cacaaggttg acgtacgcga
     2641 tgcaactggt atcgacccgg ttagcctgat cgccttcgac aaagtcgtaa tgactgctga
     2701 tgctgttaag caagttgagg agatgctggc atgattcgtg aagaacgtct gctgaaggtg
     2761 ctgcgtgcac cgcacgtttc tgaaaaagcg tctactgcga tggaaaaatc caacaccatc
     2821 gtactcaaag ttgctaaaga cgcgaccaaa gcagaaatca aagctgctgt gcagaaactg
     2881 tttgaagtcg aagtcgaagt cgttaacacc ctggtagtta aagggaaagt taaacgtcac
     2941 ggacagcgta tcggtcgtcg tagcgactgg aaaaaagctt acgtcaccct gaaagaaggc
     3001 cagaatctgg acttcgttgg cggcgctgag taagtcggag gagtaataca atggcagttg
     3061 ttaaatgtaa accgacatct ccgggtcgtc gccacgtagt taaagtggtt aaccctgagc
     3121 tgcacaaggg caaacctttt gctccgttgc tggaaaaaaa cagcaaatcc ggtggtcgta
     3181 acaacaatgg ccgtatcacc actcgtcata tcggtggtgg ccacaagcag gcttaccgta
     3241 ttgttgactt caaacgcaac aaagacggta tcccggcagt tgttgaacgt cttgagtacg
     3301 atccgaaccg ttccgcgaac atcgcgctgg ttctgtacaa agacggtgaa cgccgttaca
     3361 tcctggcccc taaaggcctg aaagctggcg accagattca gtctggcgtt gatgctgcaa
     3421 tcaaaccagg taacaccctg ccgatgcgca acatcccggt tggttctact gttcataacg
     3481 tagaaatgaa accaggtaaa ggcggtcagc tggcacgttc cgctggtact tacgttcaga
     3541 tcgttgctcg tgatggtgct tatgtcaccc tgcgtctgcg ttctggtgaa atgcgtaaag
     3601 tagaagcaga ctgccgtgca actctgggcg aagttggcaa tgctgagcat atgctgcgcg
     3661 ttctgggtaa agcaggtgct gcacgctggc gtggtgttcg tccgaccgtt cgcggtaccg
     3721 cgatgaaccc ggtagaccac ccacatggtg gtggtgaagg tcgtaacttt ggtaagcacc
     3781 cggtaactcc gtggggcgtt cagaccaaag gtaagaagac ccgcagcaac aagcgtactg
     3841 ataaattcat cgtacgtcgc cgtagcaaat aattttagag gataagccat gccacgttct
     3901 ctcaagaaag gtccttttat tgacctgcac ttgctgaaga aggtagagaa agcggtggaa
     3961 agcggagaca agaagcccct gcgcacttgg tcccgtcgtt caacgatctt tcctaacatg
     4021 atcggtttga ccatcgctgt ccataatggt cgtcagcacg ttccggtatt tgtgaccgac
     4081 gaaatggtcg gtcacaaact gggtgaattc gcaccgactc gtacttatcg cggccacgct
     4141 gctgataaaa aagcgaagaa gaaataaggt aggaggaaga gatggaaact atcgctaaac
     4201 atcgccatgc tcgttcttct gctcagaagg ttcgccttgt tgctgacctg attcgcggta
     4261 agaaagtgtc gcaggctctg gatattttga cctacaccaa caagaaagcg gctgtactgg
     4321 tcaagaaagt tctggaatct gccattgcta acgctgaaca caacgatggc gctgacattg
     4381 acgatctgaa agttacgaaa attttcgtag acgaaggccc gagcatgaag cgcattatgc
     4441 cgcgtgcaaa aggtcgtgca gatcgcatcc tgaagcgcac cagccacatc actgtggttg
     4501 tgtccgatcg ctgagactct ggagactagc aatgggtcag aaagtacatc ctaatggtat
     4561 tcgcttgggt attgtaaaac catggaactc tacctggttt gcgaacacca aagaattcgc
     4621 tgacaacctg gacagcgatt ttaaagtacg tcagtacctg actaaggaac tggctaaagc
     4681 gtctgtatct cgtatcgtta tcgagcgtcc ggctaagagc atccgtgtaa ccattcacac
     4741 tgctcgcccg ggtatcgtta tcggtaaaaa aggtgaagac gtagaaaaac tgcgtaaggt
     4801 cgtagcggac atcgctggcg ttcctgcaca gatcaacatc gccgaagttc gtaagcctga
     4861 actggacgca aaactggttg ctgacagcat cacttctcag ctggaacgtc gcgttatgtt
     4921 ccgtcgtgct atgaagcgtg ctgtacagaa cgcaatgcgt ctgggcgcta aaggtattaa
     4981 agttgaagtt agcggccgtc tgggcggcgc ggaaatcgca cgtaccgaat ggtaccgcga
     5041 aggtcgcgta ccgctgcaca ctctgcgtgc tgacatcgac tacaacacct ctgaagcgca
     5101 caccacttac ggtgtaatcg gcgttaaagt gtggatcttc aaaggcgaga tcctgggtgg
     5161 tatggctgct gttgaacaac cggaaaaacc ggctgctcag cctaaaaagc agcagcgtaa
     5221 aggccgtaaa taaggagcgt cgctgatgtt acaaccaaag cgtacaaaat tccgtaaaat
     5281 gcacaaaggc cgtaaccgcg gtctggcgca gggtacggat gttagcttcg gcagcttcgg
     5341 tctgaaagct gttggccgtg gtcgtctgac tgcccgtcag atcgaagcag cacgtcgtgc
     5401 tatgacccgt gcagttaagc gtcaaggtaa gatctggatc cgtgtgttcc cggacaaacc
     5461 gatcactgaa aagccgctgg cagtgcgtat gggtaaaggt aaaggtaacg tggagtattg
     5521 ggttgccttg attcagccgg gtaaagtcct gtatgaaatg gacggtgttc cggaagagct
     5581 ggcccgtgaa gcattcaagc tggcagcagc gaaactgccg attaaaacca cctttgtaac
     5641 taagacggtg atgtaatgaa agcaaaagag ctgcgtgaga agagcgttga agagctgaac
     5701 accgagctgc tgaacctgct gcgtgagcag ttcaacctgc gtatgcaggc tgcaagtggc
     5761 cagctgcaac agtctcacct gttgaagcaa gtgcgtcgcg atgtcgcacg cgttaagact
     5821 ttactgaacg agaaggcggg tgcgtaatga ccgataaaat ccgtactctg caaggtcgcg
     5881 ttgttagcga caaaatggag aaatccattg ttgttgctat cgaacgtttt gtgaaacacc
     5941 cgatctacgg taaattcatc aagcgtacga ccaaactgca cgtacatgac gagaacaacg
     6001 aatgcggtat cggtgacgtg gttgaaatcc gcgaatgccg tccgctgtcc aagactaaat
     6061 cctggacgct ggttcgcgtt gtagagaaag cggttctgta atacagtaca ctctctcgat
     6121 acgaataaac ggctcagaaa tgagccgttt attttttcta cccatatcct tgaagcggtg
     6181 ttataatgcc gcgccctcga tatggggatt tttaacgacc tgattttcgg gtctcagtag
     6241 tagttgacat tagcggagca ctaaaatgat ccaagaacag actatgctga acgtcgccga
     6301 caactccggt gcacgtcgcg taatgtgtat caaggttctg ggtggctcgc accgtcgcta
     6361 cgcaggcgta ggcgacatca tcaagatcac catcaaagaa gcaattccgc gtggtaaggt
     6421 caaaaaaggt gatgtgctga aggcggtagt ggtgcgcacc aagaagggtg ttcgtcgccc
     6481 ggacggttct gtcattcgct tcgatggtaa tgcttgtgtt cttctgaaca acaacagcga
     6541 gcagcctatc ggtacgcgta tttttgggcc ggtaactcgt gagcttcgta gtgagaagtt
     6601 catgaaaatt atctctctgg caccagaagt actctaagga gcgaatcatg gcagcgaaaa
     6661 tccgtcgtga tgacgaagtt atcgtgttaa ccggtaaaga taaaggtaaa cgcggtaaag
     6721 ttaagaatgt cctgtcttcc ggcaaggtca ttgttgaagg tatcaacctg gttaagaaac
     6781 atcagaagcc ggttccggcc ctgaaccaac cgggtggcat cgttgaaaaa gaagccgcta
     6841 ttcaggtttc caacgtagca atcttcaatg cggcaaccgg caaggctgac cgtgtaggct
     6901 ttagattcga agacggcaaa aaagtccgtt tcttcaagtc taacagcgaa actatcaagt
     6961 aatttggagt agtacgatgg cgaaactgca tgattactac aaagacgaag tagttaaaaa
     7021 actcatgact gagtttaact acaattctgt catgcaagtc cctcgggtcg agaagatcac
     7081 cctgaacatg ggtgttggtg aagcgatcgc tgacaaaaaa ctgctggata acgcagcagc
     7141 agacctggca gcaatctccg gtcaaaaacc gctgatcacc aaagcacgca aatctgttgc
     7201 aggcttcaaa atccgtcagg gctatccgat cggctgtaaa gtaactctgc gtggcgaacg
     7261 catgtgggag ttctttgagc gcctgatcac tattgctgta cctcgtattc gtgacttccg
     7321 tggcctgtcc gctaagtctt tcgacggtcg tggtaactac agcatgggtg tccgtgagca
     7381 gatcatcttc ccagaaatcg actacgataa agtcgaccgc gttcgtggtt tggatattac
     7441 cattaccact actgcgaaat ctgacgaaga aggccgcgct ctgctggctg cctttgactt
     7501 cccgttccgc aagtaaggta gggttactaa atggctaagc aatcaatgaa agcacgcgaa
     7561 gtaaaacgcg tagctttagc tgataaatac ttcgcgaaac gcgctgaact gaaagcgatc
     7621 atctctgatg tgaacgcttc cgacgaagat cgttggaacg ctgttctcaa gctgcagact
     7681 ctgccgcgtg attccagccc gtctcgtcag cgtaaccgct gccgtcaaac aggtcgcccg
     7741 catggtttcc tgcggaagtt cgggttgagc cgtattaagg tccgtgaagc cgctatgcgc
     7801 ggtgaaatcc cgggtctgaa aaaggctagc tggtaattgt caccaattga atcacgggag
     7861 taaagacaga tgagcatgca agatccgatc gcggatatgc tgacccgtat ccgtaacggt
     7921 caggccgcga acaaagctgc ggtcaccatg ccttcctcca agctgaaagt ggcaatcgcc
     7981 aacgtgctga aggaagaagg ttttattgaa gattttaaag ttgaaggcga caccaagcct
     8041 gaactggaac ttactctgaa gtatttccag ggcaaagctg ttgtagaaag cattcagcgt
     8101 gtcagccgcc caggtctgcg catctataaa cgtaaagatg agctgccgaa agttatggcg
     8161 ggtctgggta tcgcagttgt ttctacctct aaaggtgtta tgactgatcg tgcagcgcgc
     8221 caggctggtc ttggtggcga aattatctgc tacgtagcct aatcggagga aaaaatgtct
     8281 cgtgttgcta aagcaccggt cgttgttcct gccggcgttg acgtaaaaat caacggtcag
     8341 gttattacga tcaaaggtaa aaacggcgag ctgactcgta ctctcaacga tgctgttgaa
     8401 gttaaacatg cagataatac cctgaccttc ggtccgcgtg atggttacgc agacggttgg
     8461 gcacaggctg gtaccgcgcg tgccctgctg aactcaatgg ttatcggtgt taccgaaggc
     8521 ttcactaaga agctgcagct ggttggtgta ggttaccgtg cagcggttaa aggcaatgtg
     8581 attaacctgt ctctgggttt ctctcatcct gttgaccatc agctgcctgc gggtatcact
     8641 gctgaatgtc cgactcagac tgaaatcgtg ctgaaaggcg ttgataagca ggtgatcggc
     8701 caggttgcag cggatctgcg cgcctaccgt cgtcctgagc cttataaagg caagggtgtt
     8761 cgttacgccg acgaagtcgt gcgtaccaaa gaggctaaga agaagtaagg taacactatg
     8821 gataagaaat ttgctcgtat ccgtcgtgcg acccgcgcac gccgcaagct ccaggagctg
     8881 ggcgcaactc gcctggtggt acatcgtacc ccgcgtcaca tttacgcaca ggtaattgca
     8941 ccgaacggtt ctgaagttct ggtagctgct tctactgtag aaaaagctat cgctgaacaa
     9001 ctgaagtaca ccggtaacaa agacgcggct gcagctgtgg gtaaagctgt cgctgaacgc
     9061 gctctggaaa aaggcatcaa agatgtatcc tttgaccgtt ccgggttcca atatcatggt
     9121 cgtgtccagg cactggcaga tgctgcccgt gaagctggcc ttcagttcta aggtagaggt
     9181 gtaagatggc tcacatcgaa aaacaagctg gcgaactgca ggaaaagctg atcgcggtaa
     9241 accgcgtatc taaaaccgtt aaaggtggtc gtattttctc cttcacagct ctgactgtag
     9301 ttggcgatgg taacggtcgc gttggttttg gttacggtaa agcgcgtgaa gttccagcag
     9361 cgatccagaa agcgatggaa aaagcccgtc gcaatatgat taacgtcgcg ctgaataacg
     9421 gcactctgca acaccctgtt aaaggtgttc acacgggttc tcgcgtattc atgcagccgg
     9481 cttccgaagg taccggtatc atcgccggtg gtgcaatgcg cgccgttctg gaagtcgctg
     9541 gggttcataa cgttctggct aaagcatatg gttccaccaa cccgatcaac gtggttcgtg
     9601 caactattga tggcctggaa aatatgaatt ctccagaaat ggtcgctgcc aagcgtggta
     9661 aatccgttga agaaattctg gggaaataaa ccatggcaaa gactattaaa attactcaaa
     9721 cccgcagtgc aatcggtcgt ctgccgaaac acaaggcaac gctgcttggc ctgggtctgc
     9781 gtcgtattgg tcacaccgta gagcgcgagg atactcctgc tattcgcggt atgatcaacg
     9841 cggtttcctt catggttaaa gttgaggagt aagagatgcg tttaaatact ctgtctccgg
     9901 ccgaaggctc caaaaaggcg ggtaaacgct tgggtcgtgg tatcggttct ggcctcggta
     9961 aaaccggtgg tcgtggtcac aaaggtcaga agtctcgttc tggcggtggc gtacgtcgcg
    10021 gtttcgaggg tggtcagatg cctctgtacc gtcgtctgcc gaaattcggc ttcacttctc
    10081 gtaaagcagc gattacagcc gaagttcgtc tgtctgacct ggctaaagta gaaggcggtg
    10141 ttgtagacct gaacacgctg aaagcggcta acattatcgg tatccagatc gagttcgcga
    10201 aagtgatcct ggctggcgaa gtaacgactc cggtaactgt tcgtggcctg cgtgttacta
    10261 aaggcgctcg tgctgctatc gaagctgctg gcggtaaaat cgaggaataa gtagcagatg
    10321 gctaaacaac cgggattaga ttttcaaagt gccaaaggtg gcttaggcga gctgaaacgc
    10381 agactgctgt ttgttatcgg tgcgctgatt gtgttccgta ttggctcttt tattccgatc
    10441 cctggtattg atgccgctgt acttgccaaa ctgcttgagc aacagcgagg caccatcatt
    10501 gagatgttta acatgttctc tggtggtgct ctcagccgtg cttctatctt tgctctgggg
    10561 atcatgccgt atatttcggc gtcgatcatt atccagctgc tgacggtggt tcacccaacg
    10621 ttggcggaaa ttaagaaaga aggggagtct ggtcgtcgta agatcagcca gtacacccgc
    10681 tacggtactc tggtgctggc aatattccag tcgatcggta ttgctactgg tctgccgaat
    10741 atgcctggta tgcaaggcct ggtgattaac ccgggctttg cattctactt caccgctgtt
    10801 gtaagtctgg tcacaggaac gatgttcctg atgtggttgg gcgaacagat tactgaacga
    10861 ggtatcggca acggtatttc aatcattatc ttcgtcggta ttgtcgcggg actcccgcca
    10921 gccattgccc atactatcga gcaagcgcgt caaggcgacc tgcacttcct cgtgttgctg
    10981 ttggttgcag tattagtatt tgcagtgacg ttctttgttg tatttgttga gcgtggtcaa
    11041 cgccgcattg tggtaaacta cgcgaaacgt cagcaaggtc gtcgtgttta tgctgcacag
    11101 agcacacatt taccgctgaa agtgaatatg gcgggggtaa tcccggcaat cttcgcttcc
    11161 agtattattc tgttcccggc gaccatcgcg tcatggttcg ggggcggtac tggttggaac
    11221 tggctgacaa caatttcgct gtatttgcag cctgggcaac cgctttatgt gttactctat
    11281 gcgtctgcaa tcatcttctt ctgtttcttc tacacggcgt tggttttcaa cccgcgtgaa
    11341 acagcagata acctgaagaa gtccggtgca tttgtaccag gaattcgtcc gggagagcaa
    11401 acggcgaagt atatcgataa agtaatgacc cgcctgaccc tggttggtgc gctgtatatt
    11461 acctttatct gcctgatccc ggagttcatg cgtgatgcaa tgaaagtacc gttctacttc
    11521 ggtgggacct cactgcttat cgttgttgtc gtgattatgg actttatggc tcaagtgcaa
    11581 actctgatga tgtccagtca gtatgagtct gcattgaaga aggcgaacct gaaaggctac
    11641 ggccgataat tggtcgcccg agaagttacg gagagtaaaa atgaaagttc gtgcttccgt
    11701 caagaaatta tgccgtaact gcaaaatcgt taagcgtgat ggtgtcatcc gtgtgatttg
    11761 cagtgccgag ccgaagcata aacagcgcca aggctgattt tttcgcatat ttttcttgca
    11821 aagttgggtt gagctggcta gattagccag ccaatctttt gtatgtctgt gcgtttccat
    11881 ttgagtatcc tgaaaacggg cttttcagca tggaacgtac atattaaata gtaggagtgc
    11941 atagtggccc gtatagcagg cattaacatt cctgatcata agcatgccgt aatcgcatta
    12001 acttcgattt atggcgtcgg caagacccgt tctaaagcca tcctggctgc agcgggtatc
    12061 gctgaagatg ttaagatcag tgagctgtct gaaggacaaa tcgacacgct gcgtgacgaa
    12121 gttgccaaat ttgtcgttga aggtgatctg cgccgtgaaa tcagcatgag catcaagcgc
    12181 ctgatggatc ttggttgcta tcgcggtttg cgtcatcgtc gtggtctccc ggttcgcggt
    12241 cagcgtacca agaccaacgc acgtacccgt aagggtccgc gcaaaccgat caagaaataa
    12301 tcggggtgat tgaataatgg caaaggcacc aattcgtgca cgtaaacgtg taagaaaaca
    12361 agtctctgac ggcgtggctc atatccatgc ttctttcaac aacaccatcg tgactatcac
    12421 tgatcgtcag ggtaacgcgt tgggttgggc aacagccggt ggttccggtt tccgtggttc
    12481 tcgcaaatcc actccgtttg cagctcaggt tgcagcagag cgttgcgctg acgccgtgaa
    12541 agaatacggc atcaagaatc tggaagttat ggttaaaggt ccgggtccag gccgcgaatc
    12601 tactattcgt gctctgaacg ccgcaggttt ccgcatcact aacattactg atgtgactcc
    12661 gatccctcat aacggttgtc gtccgccgaa aaaacgtcgc gtataacgcc tcgttttcca
    12721 ggtttgttgg agaaagaaaa tggcaagata tttgggtcct aagctcaagc tgagccgtcg
    12781 tgagggcacc gacttattcc ttaagtctgg cgttcgcgcg atcgatacca agtgtaaaat
    12841 tgaacaagct cctggccagc acggtgcgcg taaaccgcgt ctgtctgact atggtgtgca
    12901 gttgcgtgaa aagcaaaaag ttcgccgtat ctatggtgtg ctggagcgtc agttccgtaa
    12961 ctactacaaa gaagcagcac gtctgaaagg caacaccggt gaaaacctgt tggctctgct
    13021 ggaaggtcgt ctggacaacg ttgtataccg tatgggcttc ggtgccactc gtgcagaagc
    13081 acgtcagctg gttagccata aagcaattat ggtaaacggt cgtgttgtta acatcgcttc
    13141 ttatcaggtt agtccgaatg acgttgtaag cattcgtgag aaagcgaaga agcagtctcg
    13201 cgtgaaagcc gctctggagc tggctgagca gcgtgaaaag ccaacttggc tggaagttga
    13261 tgctggcaag atggaaggta cgtttaagcg taagccggag cgttctgatc tgtctgcgga
    13321 cattaacgaa cacctgatcg tcgagcttta ctccaagtaa agcttagtac caaagagagg
    13381 acacaatgca gggttctgtg acagagtttc taaaaccgcg cctggttgat atcgagcaag
    13441 tgagttcgac gcacgccaag gtgacccttg agcctttaga gcgtggcttt ggccatactc
    13501 tgggtaacgc actgcgccgt attctgctct catcgatgcc gggttgcgcg gtgaccgagg
    13561 ttgagattga tggtgtacta catgagtaca gcaccaaaga aggcgttcag gaagatatcc
    13621 tggaaatcct gctcaacctg aaagggctgg cggtgagagt tcagggcaaa gatgaagtta
    13681 ttcttacctt gaataaatct ggcattggcc ctgtgactgc agccgatatc acccacgacg
    13741 gtgatgtcga aatcgtcaag ccgcagcacg tgatctgcca cctgaccgat gagaacgcgt
    13801 ctattagcat gcgtatcaaa gttcagcgcg gtcgtggtta tgtgccggct tctacccgaa
    13861 ttcattcgga agaagatgag cgcccaatcg gccgtctgct ggtcgacgca tgctacagcc
    13921 ctgtggagcg tattgcctac aatgttgaag cagcgcgtgt agaacagcgt accgacctgg
    13981 acaagctggt catcgaaatg gaaaccaacg gcacaatcga tcctgaagag gcgattcgtc
    14041 gtgcggcaac cattctggct gaacaactgg aagctttcgt tgacttacgt gatgtacgtc
    14101 agcctgaagt gaaagaagag aaaccagagt tcgatccgat cctgctgcgc cctgttgacg
    14161 atctggaatt gactgtccgc tctgctaact gccttaaagc agaagctatc cactatatcg
    14221 gtgatctggt acagcgtacc gaggttgagc tccttaaaac gcctaacctt ggtaaaaaat
    14281 ctcttactga gattaaagac gtgctggctt cccgtggact gtctctgggc atgcgcctgg
    14341 aaaactggcc accggcaagc atcgctgacg agtaaccgga tcacaggtta aggttttact
    14401 gagaaggata aggtcatgcg ccatcgtaag agtggtcgtc aactgaaccg caacagcagc
    14461 catcgccagg ctatgttccg caatatggca ggttcactgg ttcgtcatga aatcatcaag
    14521 acgactctgc ctaaagcgaa agagctgcgc cgcgtagttg agccgctgat tactcttgcc
    14581 aagactgata gcgttgctaa tcgtcgtctg gcatttgccc gtactcgtga taacgagatc
    14641 gtggcaaaac tgtttaacga actgggcccg cgtttcgcga gccgtgccgg tggttacact
    14701 cgtattctga agtgtggctt ccgtgcaggc gacaacgcgc cgatggctta catcgagctg
    14761 gttgatcgtt cagagaaagc agaagctgct gcagagtaat ctgaagcaat gtaaaaaaac
    14821 ccgccccggc gggttttttt atacccgtag tatccccact tatctacaat agctgtactc
    14881 tttttgttca tcccctggag tatttatgtg gttacttgac cagtgggcag agcgccatat
    14941 agcagaagcg caagcgaaag gtgagtttga taacctggca ggtagcggcg aaccattgat
    15001 actggatgat gattctcacg tgccaccgga attacgtgcg gggtatcgct tgctgaagaa
    15061 tgccggttgc ttaccgccag aacttgagca acggagagaa gcaattcagc ttctggatat
    15121 tctcaaaggt atccgtcacg atgatccgca atatcaagag gttagccgtc gattgtcatt
    15181 actggaattg aagctgcgac aagctggatt gagtaccgat tttttacgcg gcgattatgc
    15241 tgacaagttg ttggacaaaa tcaacgataa ctagtggagt atgtatgtat cgcattggtg
    15301 agctggcaaa aatggcggaa gtaacacctg acacgattcg ttattacgaa aaacagcaga
    15361 tgatggagca tgaagtgcgt actgaaggcg ggtttcgcct gtataccgaa agcgatctcc
    15421 agcgattgaa atttatccgc catgccagac aactaggttt cagtctggag tcgatccgcg
    15481 agttgctgtc gatccgcatc gatcctgaac accatacctg tcaggagtca aaaggcattg
    15541 tgcaggaaag attgcaggaa gtcgaagcac ggatagccga gttgcagagt atgcagcgtt
    15601 ccttgcaacg ccttaacgat gcctgttgtg ggaccgccca tagcagtgtt tattgctcga
    15661 ttcttgaagc tcttgaacaa ggggcgagtg gcgttaagag tggctgttga ttttttgcac
    15721 tgacaggatt acactcgcgc cgttaaataa ccaactggag tttttatgag ccgatatcag
    15781 catactaaag ggcagataaa ggataatgcg atagaagcat tactacatga tcccttattc
    15841 cgacagcgag tagagaaaaa taagaaaggg aaaggcagtt acatgcgaaa aggaaaacat
    15901 ggcaatcggg ggaactggga ggccagtggc aagaaagtaa atcacttttt taccactggt
    15961 cttctgcttt caggcgcttg ttaagagcgg ttattctgct ctttcagcaa atcacgaatt
    16021 tctgccagta atacttcttc tttagttggt gcaggtgcgg cagctggttc ttcttttttc
    16081 cgattcagtt tgttgattag cttaatcgcc ataaagatgg caaaggccac aatcagaaaa
    16141 tcaaagacgt tttgaatgaa gacaccgtaa tgcatcacaa cagcagggat atccccctgc
    16201 gcatcgcgta gcgtgacagc aaactgttta aagtcgatcc cgccaattaa taagcccaga
    16261 ggtggcatga tgatatcggc aaccagtgaa gatacaatct tcccgaatgc cgcaccgata
    16321 atgacaccca ccgccaaatc caccacgttc ccgcgcatcg caaattcgcg aaattcttta
    16381 ataatgctca tgttattctc cctatgaagc cgacaaccat aagtctaaca aatgttaagc
    16441 cattttcctg ccaggaatga atttaattaa tccttgataa tcaaagggaa ataataaggc
    16501 gtcattagac gccttattaa ttacaagaag aaagggcttg gctggaagag tctttcgacg
    16561 tcggtaataa actttttatc tgtgaggaac ataattacgt gatcgccttg ctcaatgcgc
    16621 agattgtcat tggcaatcat cacgtcgttt ccacgtacca ccgctccaat aatcgttcct
    16681 ggcggtagct tgatttcgtc aatgactctg ccgacaacgc gtgaggtgct ttcatcaccg
    16741 tgagcaacgg cttcaatagc ttctgctacg ccgcggcgca atgaggaaac accaacaata
    16801 tctgcttttc gcacatggct aagcaacgca gaaatagttg cttgttgtgg tgaaattgca
    16861 atatcgatca cgctcccctg aaccagatcc acataagcgc gacgctggat caataccatc
    16921 acctttttcg cacccatacg tttggcaagc atggcggaca tgatattggc ctcgtcatcg
    16981 ttggtgacgg caataaacag atcaacttga tcgatatgtt cttcggccag cagttcttga
    17041 tccgacgcat caccaaaaaa gacgatcgta ttctgcaact tttccgccag ttcggcagcg
    17101 cgctgctgat tacgttcgat gagtttaacg ctgtaatctt tttccagacg acgcgccagc
    17161 cctgcaccga tattaccgcc accaaccagc atgatccgct tatacggttt ttccagtcgc
    17221 tgtaattcac tcatcaccgc gcggatatgc tgtgaagcgg caataaagaa cacttcatca
    17281 ccagcttcaa caatggtcga accttgtgga cgaatggggc gatcgtggcg gaaaattgct
    17341 gccacacgag tatcgatatg tggcatatgt tcgcgcatgg tcgaaagtgc attaccaatc
    17401 agcgggccgc cataataggc tttaactaca gcgaggctga ctttaccctc agcgaagttc
    17461 accacctgca atgcgcctgg atactcaatc agtcggtaaa tattatcgat aaccaactgc
    17521 tctggtgcga tcagatgatc aatcggtaca gcatctgaat gaaatagctt atcggcatcg
    17581 cgtacgtagt ctggtgagcg gatgcgagcg atgcgattag gggtgttgaa aagtgagtag
    17641 gctacctggc aggcaaccat attggtttca tctgaactgg ttacagcaac cagcatatcg
    17701 gcgtcgtcgg cacctgcctc ccgcaatacg cgtggatgag agccatgccc ctgcacgact
    17761 cgcaggtcaa atttatcctg caaggtccgc agacgctcac cgttggtatc gacaacagta
    17821 atatcgttgt tctcgccaac caggttttcc gccagtgtgc cgccaacctg gccggcaccc
    17881 agaatgataa ttttcatcag tctcgacccg ttatctcatc actttttgat tagcttagcg
    17941 taaaagaagc cgtcgccctc ttcggcacca ggcagatttt gtttacccgg ttgctctggt
    18001 gttcctgttt cgcaaagttc ggcatcagcg gtacgttgca aaaaagcttt aatctgcagg
    18061 ctattctctt ccggtaacac cgaacaggtg gcatagacca gggttccacc ggattttaaa
    18121 tgcggccaaa tggcgtcgag aatttcagac tgcaattgcg cgagttccgg gatatcgcga
    18181 tcgcgacgta accatttaat atccggatgg cgacgaatca cccccgttgc tgaacaaggc
    18241 gcatctaata aaatgcgatc aaactgttgc tcgccacacc attgggaagg gtaacggcca
    18301 tcaccttgtt tcacggtcgc cttcatacca aggcgtttta aattgtcgtg aacgcgagag
    18361 aggcgctgtt cgtcaatatc aaccgcaaca acctgcgctt ctggtgccac ctcaaggata
    18421 tgcgttgttt taccgccggg ggcggcacaa agatccagaa tgtgttcacc gttttgtggc
    18481 gcaagccagg tcatgcaacc ttgtgctgat gcatcctgaa cggtaaccca tccgtcttca
    18541 aaaccaggta gcgcatgaac aggtgcaggt gtttccagcc gtacagcatc agggtaatcc
    18601 gcatgcggga aacctttcat tcctgcttca tccaacaatg caagccagct gtcgcgggaa
    18661 tgatgcgtac gattaacgcg cagccacatt ggcggacgct ggttattggc ttcgacgatg
    18721 gattgccact gctctggata cgctttttgc agacgcttca gcaaccagga aggatgcaga
    18781 taacgtgcat cactggtatt aaactcggct aataactctt cttgctgacg ctggaactgg
    18841 cgtaatacac cgttaatcaa ccctttaagt tgcggacgct taattgcgat agcgccttca
    18901 accgtttcag ccagcgcagc atgaggtgga atgcgggtat aaagcagttg atacaaacca
    18961 accataatca ggtaatgcac agtgcgctgt ttgccggtca tcggacgggc cattaactta
    19021 ttaatcagcc agtctaactg cgaaagcgta cgcagtacgc caaagcacaa ctcttgaaga
    19081 agtgctttgt ctttatcaga aactttttgc tggagcggtg gcagaatgtt gcttaatgat
    19141 tgcccttgct cgacgacttg ttcaacggcc tgggccgcca tgctacgtaa attacgttgt
    19201 tttttcataa gtataaaaat gcccggtaag accgggctta gaagagtgga ctatcaggcc
    19261 agacggttgc ccggaacaaa ccattcccga cgagaattca ggaggtcttg cgcgctcatc
    19321 gctttcttac ccgcaggttg taacgagagc aggttcagga tgccatcacc agtcgcaacc
    19381 tgaatgcctt gtttgttggc ttcaaggatc gttcctggtg cagcgttggt tgccgtatca
    19441 atgaccgacg ctttccagac tttaaccggt tgtccttcaa tttccagcca gctcattggc
    19501 catgggttga aagcgcgaat gcagcgttca agctgcgctg ccgaaagcga ccagtcaata
    19561 cgcgcttctt ctttactcaa cttctcggcg taagtgacaa gagtttcgtc ctgaacttct
    19621 ggtttcgccg tgccgtctgc tagttgtttc aacgtggtga ttagcccttg tgggccaagc
    19681 tctgccagct tgtcgtacag cgtaccactg gtatcttctg cagtaatcgg gcaggagagc
    19741 ttatagagca tatcaccggt gtctaaaccg acatccattt gcataatggt cacaccagtt
    19801 tctgcatcac ccgcccatag tgagcgttgg attggtgcag caccgcgcca gcgtggcagc
    19861 agtgaaccat gaacgttgat acagccaaga cgcggcatct ccagcactgc tttaggcaga
    19921 attaagccat aggcgacgac gaccataaca tcagcctgca gatcggcgac cagttgctgg
    19981 ctttcttgtg gacgcaggga aacaggttga aaaacgggca gacctttttc ctcagccaga
    20041 actttaaccg ggctgggcat cagtttttta ccgcgtcctg ccggtcggtc tggctgggtg
    20101 aacacgccaa cgacgttatg accagaagac aacagcgcgt cgagatgacg cgctgcaaag
    20161 tcaggtgtac ccgcaaaaat aatacgtagt gattctgaca cgttagttct tatccttaag
    20221 cccgggcttt cagacgatct agtttttcaa ctttctgacg aatacgttgt tgtttcagcg
    20281 gtgacagata atccataaac agtttgccga ccaggtgatc catctcatgc tgaatacaga
    20341 tggctaacag cccctctgct tccagttcaa atggtttacc gtcgcggtca agggcgcgaa
    20401 ttttaacttt ctctgcgcgc ggcactaaag cacgttgttc agggatcgac aggcaacctt
    20461 cttcaatgcc tgtttcgccg cttttttcta aaagctccgg attgattaac accagccgtt
    20521 cgtcacggtt ttccgaaaca tcaataacaa tgatacgttg atggatatca acctgggttg
    20581 ccgccaggcc aataccttct tctgcgtaca tcgtctcgaa catatcatcg acgatacgct
    20641 gtatttctgc attcacttct tctaccggtt tagcaacttt gcgaagccgc tcgtccggaa
    20701 tatgtaacac ttgcaaaact gacataaatc tccagagatg tgttcagggg ttagaaagat
    20761 tatttcttct attctagaca aatccccctc tgattgacag catcactgac caatcgcaaa
    20821 gattgctaag gctgcttatg gcagggagat aaggatggtc gatacagaaa tttggctgcg
    20881 tttaatgagt atcagcagct tgtacggcga tgatatggtc cgtatagctc actggctggc
    20941 aaaacagtcg caaattgatg cggttggatt gcagcaaaca gggcttacat tgcggcaggc
    21001 acaacgcttt ctttcatttc cgcgaaagag tatcgaaagc tcactttgtt ggttggagca
    21061 acccaaccat catttaatcc ctgcggacag cgaattttat cctcctcaac ttctggtgac
    21121 gacagattac cccggcgcac tgtttgttga aggagaactg cacgcgctgc attcatttca
    21181 gcttgccgta gtggggagtc gggcgcattc atggtatggc gagcgatggg gacggttatt
    21241 ttgcgaaact ctggcgacgc gtggagtgac aattacgagt ggactggcgc gtggaatcga
    21301 tggtgtggcg cataaagctg ccttacaggt aaatggcgtc agcattgctg tattggggaa
    21361 tggacttaat accattcatc cccgccgcca tgcccgactg gctgccagtc tgcttgaaca
    21421 tggcggagct ctcgtctcgg aatttcccct cgatgttcca ccccttgctt acaatttccc
    21481 acgaagaaat cgcattatca gtggtctaag taaaggtgta ctggtggtgg aagcggcttt
    21541 gcgcagtggt tcgctggtga cagcacgttg tgcgcttgag cagaggcgtg aagtttttgc
    21601 cttgccaggt ccaataggga atccgggaag cgaagggcct cactggttaa taaaacaagg
    21661 tgcgattctt gtgacggaac cggaagaaat tctggaaaac ttgcaatttg gattgcactg
    21721 gttgccagac gcccctgaaa attcatttta ttcaccagat cagcaagacg tggcattgcc
    21781 atttcctgag ctcctggcta acgtaggaga tgaggtaaca cctgttgacg tcgtcgctga
    21841 acgtgccggc caacctgtgc cagaggtagt tactcaacta ctcgaactgg agttagcagg
    21901 atggatcgca gctgtacccg gcggctatgt ccgattgagg agggcatgcc atgttcgacg
    21961 tactaatgta tttgtttgaa acctatattc acactgaagc tgagttgcgt gtggatcaag
    22021 acaaacttga acaggatctt accgacgcag gttttgagcg agaagatatc tacaatgccc
    22081 tgctatggct ggaaaaactt gctgattatc aggaagggtt ggcagaaccg atgcaactgg
    22141 cctcggatcc tctctccatg cgtatttata caccggaaga gtgtgaacga ctggatgcca
    22201 gctgccgtgg gtttctgctt ttccttgagc agattcaggt gctcaacctt gaaactcgtg
    22261 aaatggtgat agagcgagtg ctcgcgctgg ataacgcaga gttcgaactg gatgatctga
    22321 aatgggtgat cctgatggtg ttgttcaaca tcccggggtg cgaaaatgcg taccagcaaa
    22381 tggaagaatt actctttgaa gtgaatgaag gtatgctgca ttaatcattt ctttaattca
    22441 gcataagttg ttatggcaaa atcagcactg ttcacggtgc gtaataatga gtcctgccca
    22501 aagtgcgggg ctgaactggt tattcgatcc gggaaacacg gtccgtttct tggatgctca
    22561 cagtatccgg cgtgtgacta cgtccgtctt ctgaaatctt cagcggatgg acatattgtc
    22621 aaagttctgg aggggcaggt ttgccctgca tgtggcgcaa atctggtatt acgccaggga
    22681 cgctttggta tgtttattgg ttgcagtaac taccctgaat gcgaacatac cgaacttatc
    22741 gataaaccgg acgaaacagc aattacatgc ccccaatgtc ggacgggcca tctggtccag
    22801 cgccgctccc gttatggcaa aacatttcac tcctgtgatc gctacccgga gtgtcaattt
    22861 gccattaact tcaaaccaat agctggagaa tgccctgagt gtcattatcc gctactcatc
    22921 gaaaagaaaa ccgcgcaggg tgtaaaacac ttttgtgcca gtaaacaatg tggaaagccg
    22981 gtttcggcgg aataataacg tgaataataa cctgcaagga gacgctatcg cagctgcgat
    23041 agatgtcctc aatgaagaac gtgtcatcgc ctatccaacg gaagccgttt tcggtgttgg
    23101 gtgcgatcct gatagcgaaa cagcagtgat gagactgttg gagttaaaac agcgtccggt
    23161 tgataagggg ctgattttaa tcgcggcaaa ttacgagcag cttaaaccct atattgatga
    23221 caccatgctg actgatgcgc agcgtgaaac cattttttcc cgctggccag gtcctgtcac
    23281 ctttgtcttt cccgcgcctg cgacaacacc gcgctggttg acgggccgct ttgattcgct
    23341 tgctgtacga gtcaccgacc atccgttggt ggttgctttg tgccaggctt atggtaaacc
    23401 gctggtttct accagtgcca acttgagtgg attgccacct tgtcgaacag tagacgaagt
    23461 tcgcgcacaa tttggcgcgg cgttcccggt tgtgcctggt gaaacggggg ggcgtttaaa
    23521 tccttcagaa atccgcgatg ccctgacggg tgaactgttt cgacaggggt aacataatgg
    23581 aaacctatgc tgtttttggt aatccgatag cccacagcaa atcgccattc attcatcagc
    23641 aatttgctca gcaactgaat attgaacatc cctatgggcg cgtgttggca cccatcaatg
    23701 atttcatcaa cacactgaac gctttctttc gtgctggtgg taaaggtgcg aatgtgacgg
    23761 tgccttttaa agaagaggct tttgccagag cggatgagct tactgaacgg gcagcgttgg
    23821 ctggtgcagt taataccctc aagcggttag aagatggacg cctgctgggt gacaataccg
    23881 atggtgtagg cttgttaagc gatctggaac gtctgtcttt tatccgccct ggtttacgta
    23941 ttctgcttat cggcgctggt ggagcatctc gcggcgtact actgccactt ctttccctag
    24001 actgtgcggt gacaatcact aatcggacgg tatcccgcgc ggaagagttg gctaaattgt
    24061 ttgcgcacac tggcagtatt caggcgttgg gtatggacga actggaaggt catgagtttg
    24121 atctcattat taatgcaaca tccagtggca tcagtggtga tattccggcg atcccgtcat
    24181 cgctcattca tccaggcatt tattgctatg acatgttcta tcagaaagga aaaactcctt
    24241 ttctggcatg gtgtgagcag cgaggctcaa agcgtaatgc tgatggttta ggaatgctgg
    24301 tggcacaggc ggctcatgcc ttccttcttt ggcatggcgt tctgcctgat gtagaaccgg
    24361 tcatcaaact attgcaacag gaattgtcag cgtgaatcag gccatccagt ttccggatag
    24421 ggaagagtgg gacgagaata aaagatgtgt atgttttcct gctctcgtga atggtatgca
    24481 actgacatgt gcgatctctg gcgagagtct ggcgtatcgc tttactggag gtacgccaga
    24541 acagtggtta gcgagttttc gtcagcatcg ctgggacctg gaagaagaag cggaaaactt
    24601 aattcaggaa caaagtgaag atgatcaagg ttgggtctgg ttaccttgat ccagatattc
    24661 gtccttccat ttcacgtagt tatttgcgga atagcgtaac ccagccttct cttcatcact
    24721 taacgggcgg atctgtttga cgggactacc gagatacaga tatccgctct ccagccgttt
    24781 attttgtggg accaggctac ccgcaccaat catcacatca tcttctacta ttgcgccatc
    24841 aagtaaaatt gagcccatcc caaccaaaac tcgattgcca atggtgcagc cgtggagcat
    24901 caccttgtga ccaacagtga catcttcgcc aatggttaat gggttgccat ctgggttgta
    24961 cgaggattta tgagtgacat gcaacatact gccatcctgg atattggtgc gtgctccgat
    25021 ctgtacataa tgtacatctc cacgaatcac aacgagcggc cagatcccca catcatcagc
    25081 cagacgaacg tcaccaatca cgacgctgct atcgtcgatc attacgcgct ggccgatttg
    25141 tggaaaaaga tcgcggtatg ggcgtaaaac atcagacata ctcacctcaa caataaatga
    25201 tttcctaatg actttgggtg cattattagc cttgtgcaag tcttttagta tgcaaaaaag
    25261 caccgttttg tgtgcgaatg cagcaaaaag ggtgaaaaaa caacaaacag aaaaaaagat
    25321 caaaaaagta cttgtgcaaa aaattgggat ccctataatg cgcctccgtt gagacgacaa
    25381 cgtgaaacac ttcacaggat ggtcggaaca acgaagagaa aaaattctga aattcagggt
    25441 tgactctgaa agaggaaagc gtaatatacg ccacctcgcg acagtgagct gaaagccgcg
    25501 tcgcaactgc tctttaacaa tttatcagac aatctgtgtg ggcactcgaa gatacggatt
    25561 cttaacgtcg caagacgaaa aatgaatacc aagtctcaag agtgaacacg taattcatta
    25621 cgaagtttaa ttctttgagc atcaaacttt taaattgaag agtttgatca tggctcagat
    25681 tgaacgctgg cggcaggcct aacacatgca agtcgaacgg taacaggaag cagcttgctg
    25741 cttcgctgac gagtggcgga cgggtgagta atgtctggga aactgcctga tggaggggga
    25801 taactactgg aaacggtagc taataccgca taatgtcgca agaccaaaga gggggacctt
    25861 cgggcctctt gccatcggat gtgcccagat gggattagct tgttggtg
//
LOCUS       DADMKF010000049        25647 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.050, whole genome shotgun sequence.
ACCESSION   DADMKF010000049 DADMKF010000000
VERSION     DADMKF010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25647
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.050"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(76..807)
                     /gene="rlmB"
                     /locus_tag="JNC24_001849"
     CDS             complement(76..807)
                     /gene="rlmB"
                     /locus_tag="JNC24_001849"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HAY9158604.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(987..3428)
                     /gene="rnr"
                     /locus_tag="JNC24_001850"
     CDS             complement(987..3428)
                     /gene="rnr"
                     /locus_tag="JNC24_001850"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HAY9158605.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYVPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(3467..3892)
                     /gene="nsrR"
                     /locus_tag="JNC24_001851"
     CDS             complement(3467..3892)
                     /gene="nsrR"
                     /locus_tag="JNC24_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HAY9158606.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(4097..5395)
                     /gene="purA"
                     /locus_tag="JNC24_001852"
     CDS             complement(4097..5395)
                     /gene="purA"
                     /locus_tag="JNC24_001852"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HAY9158607.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPTELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVATTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(5499..5696)
                     /locus_tag="JNC24_001853"
     CDS             complement(5499..5696)
                     /locus_tag="JNC24_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HAY9158608.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(5778..6782)
                     /gene="hflC"
                     /locus_tag="JNC24_001854"
     CDS             complement(5778..6782)
                     /gene="hflC"
                     /locus_tag="JNC24_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HAY9158609.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(6785..8044)
                     /gene="hflK"
                     /locus_tag="JNC24_001855"
     CDS             complement(6785..8044)
                     /gene="hflK"
                     /locus_tag="JNC24_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HAY9158610.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(8130..9410)
                     /gene="hflX"
                     /locus_tag="JNC24_001856"
     CDS             complement(8130..9410)
                     /gene="hflX"
                     /locus_tag="JNC24_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HAY9158611.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(9487..9795)
                     /gene="hfq"
                     /locus_tag="JNC24_001857"
     CDS             complement(9487..9795)
                     /gene="hfq"
                     /locus_tag="JNC24_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HAY9158612.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSCAQNTSAQQDSEETE
                     "
     gene            complement(9881..10831)
                     /gene="miaA"
                     /locus_tag="JNC24_001858"
     CDS             complement(9881..10831)
                     /gene="miaA"
                     /locus_tag="JNC24_001858"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HAY9158613.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(10824..12671)
                     /gene="mutL"
                     /locus_tag="JNC24_001859"
     CDS             complement(10824..12671)
                     /gene="mutL"
                     /locus_tag="JNC24_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HAY9158614.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMNVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFNHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRSIPENRVAAGRNHFAEPAAREPVAPRYTPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKLKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLALPVAERWLRQVQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(12681..14018)
                     /gene="amiB"
                     /locus_tag="JNC24_001860"
     CDS             complement(12681..14018)
                     /gene="amiB"
                     /locus_tag="JNC24_001860"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HAY9158615.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADVPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKQSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(14037..14498)
                     /gene="tsaE"
                     /locus_tag="JNC24_001861"
     CDS             complement(14037..14498)
                     /gene="tsaE"
                     /locus_tag="JNC24_001861"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HAY9158616.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(14470..16017)
                     /gene="nnr"
                     /locus_tag="JNC24_001862"
     CDS             complement(14470..16017)
                     /gene="nnr"
                     /locus_tag="JNC24_001862"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HAY9158617.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADVLGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKPGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPGEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            16016..17155
                     /gene="queG"
                     /locus_tag="JNC24_001863"
     CDS             16016..17155
                     /gene="queG"
                     /locus_tag="JNC24_001863"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HAY9158618.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCIACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGAIPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAQ
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILAALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(17138..17191)
                     /locus_tag="JNC24_001864"
     CDS             complement(17138..17191)
                     /locus_tag="JNC24_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9158619.1"
                     /translation="MRFYFYSQAVDESGVTR"
     gene            complement(17425..17500)
                     /locus_tag="JNC24_001865"
     tRNA            complement(17425..17500)
                     /locus_tag="JNC24_001865"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17465..17467),aa:Gly,seq:gcc)
     gene            complement(17536..17611)
                     /locus_tag="JNC24_001866"
     tRNA            complement(17536..17611)
                     /locus_tag="JNC24_001866"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17576..17578),aa:Gly,seq:gcc)
     gene            complement(17769..17844)
                     /locus_tag="JNC24_001867"
     tRNA            complement(17769..17844)
                     /locus_tag="JNC24_001867"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17809..17811),aa:Gly,seq:gcc)
     gene            complement(18055..18600)
                     /gene="orn"
                     /locus_tag="JNC24_001868"
     CDS             complement(18055..18600)
                     /gene="orn"
                     /locus_tag="JNC24_001868"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418586.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HAY9158620.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            18695..19747
                     /gene="rsgA"
                     /locus_tag="JNC24_001869"
     CDS             18695..19747
                     /gene="rsgA"
                     /locus_tag="JNC24_001869"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HAY9158621.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            19844..20812
                     /gene="psd"
                     /locus_tag="JNC24_001870"
     CDS             19844..20812
                     /gene="psd"
                     /locus_tag="JNC24_001870"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HAY9158622.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKICQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            20834..24157
                     /gene="mscM"
                     /locus_tag="JNC24_001871"
     CDS             20834..24157
                     /gene="mscM"
                     /locus_tag="JNC24_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HAY9158623.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNMRDKPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(24308..>25647)
                     /gene="yjeM"
                     /locus_tag="JNC24_001872"
     CDS             complement(24308..>25647)
                     /gene="yjeM"
                     /locus_tag="JNC24_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HAY9158624.1"
                     /translation="MMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWM
                     VSTSAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIA
                     RITAVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVF
                     AIFAYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLS
                     NGSVNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTL
                     CYSPLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASAF
                     FNKLTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTRMSAMIAMVVVVLVVTF
                     ANVFTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRMANKPELALD"
BASE COUNT         5934 a         7193 c         6500 g         6020 t
ORIGIN      
        1 tcaccgtaat tatcttacca gctatagaca aaaaaaacca tccaaatctg gatggctttt
       61 cataattcag acgaactagc tgcgctgacg aaccgcttca aataagcaaa ttccggttgc
      121 aaccgaaacg ttcagggaag aaacacttcc tgccattggg atgctgatca actcatcgca
      181 atgttcacgg gtcaggcgac gcataccttc accttccgcg cccatcacca gcgccaagcg
      241 tccggtcatt ttgctctgat agagtgtatg atccgcctcg cctgccgtac cgacgatcca
      301 gatattctct tcctgcaaca tacgcatggt gcgcgcaagg ttagtcaccc gaatcagtgg
      361 aacgctttct gccgcgccgc aggctacttt tttcgccgta gcgttgagct gtgcggagcg
      421 atctttcggc acaatcaccg cgtgaacgcc agcagcgtcc gcgctacgca ggcacgcgcc
      481 gaggttgtgc ggatcggtta caccgtcgag gatcagcagg aacggttgat cgagcgaagc
      541 gatcagatcc ggcagatcgt tttcctgata ctgacgtcct ggcttcacgc gggcgataat
      601 gccctgatgc acggcaccgt cgcttttctc gtcgagatat tggcggtttg ccaactggat
      661 aaccacgccc tgagactcaa gggcgtgaat cagcggtaac agacgtttat cttcacggcc
      721 ttttaaaata aagacttcct gaaaacgttc aggggcgcgc tccagcaggg cctgcactgc
      781 gtggatgccg taaatcattt cgctcattaa tgtactcgtt gtttacgttt tgcctgatgc
      841 actacgttta tccggcctac atgatctctg caatatattg aatttgcgtg attatgtagg
      901 ccggataagg cgttcacgcc gcatccggca tgaacaaagc gcattttgtc agcaatctaa
      961 ccctcttctt ttaaagaggg tattgatcac tctgccactt ttttcttcgc cgcacgcttc
     1021 gctttggtcg ctgcggctat tttctgcgtt ttcgccgatg gctttttcgc ttttctcgcg
     1081 tctttcttcg ccgctttcgg cttcgttttt ttctcaccgc ggaaggcgct gtctggctca
     1141 aagtttacct ttttaccgac ctgacgacgc ttgccgcctt ttttgcctgc atcgcctttt
     1201 ttcgctttct cgcgcgccgt tttaccgacg ttgcgcggtg cgcgttcact ggagatcaga
     1261 ctaaagtcga ttttgcgctc gtccatatta accgcttcga cgcgaacttc cacgcgatcg
     1321 cccaggcgat aagtctggcc gctggattcc cccatcaggc gttgccctac ctggtcaaag
     1381 cgatagtagt cattgtccag cgaagagaca tggaccagac catcaatgaa caagtcgtcc
     1441 agacggacga agaagccaaa gccagtgacg ctggaaatta cgcctttaaa gacgttacct
     1501 acctggtcga gcatgaagtc acacttcagc cagtcagcga catcgcgcgt tgcttcgtcg
     1561 gcacgacgtt ccgccatcga acagtgctga cccagttgca acatctcttc catcgaataa
     1621 tggtagccgc cggtttcagt ggtgttgccc tgatgcccct gctctttcgc cagcagatac
     1681 ttaatggcgc ggtgcagcgt caggtcagga taacgacgaa tcggcgaagt aaagtgcgca
     1741 taggactgca atgccaggcc aaagtgacca cggttttctg gatcgtaaat cgcctgtttc
     1801 atcgagcgca gcagcatggt ttgcagcatt tctgcatcag gacggtcggc aaccgactcc
     1861 agcagttccg cgtagtcacg cggttccggc ttgttaccac ccggcagctc cagccccagc
     1921 tccgccagca ctgaacggaa agaggtaatc gcttcggtgc tcggcttgtc gtgaatacgg
     1981 aacagtgccg gttctttcgc tttctcaacg aaacgcgccg ccgagatatt cgccagaatc
     2041 atgcactctt caattaattt gtgcgcgtcg ttacgctggg tctgttcgat acgttcaata
     2101 cggcgttcag cgttgaaaat gaacttcgct tcttcgctct caaatgagat cccaccgcgt
     2161 tcttcacggg ctttatccag cactttatag aggttatgca actcttcgag atgcttaacc
     2221 agcgggacgt actgctcgcg cagatcctga tcgccctgca gaatatgcca gactttggtg
     2281 taggtcagac gcgcgtgaga gctcatcacc gcttcgtaga atttgtagcc cgtcaggcgg
     2341 cctttcgacg aaaccgtcat ctcgcacacc atacacaggc ggtctacctg cgggttgagc
     2401 gaacacaggc cgttagagag cacttccggc agcatcggga taacctgcga agggaagtac
     2461 accgacgtgc cacggttacg cgcttctctg tccagcggcg ttggcggacg cacatagtag
     2521 ctgacgtcgg caatcgcgac ccataaacgc cagccgccgc cgcgtttttt ctcgcagtaa
     2581 actgcatcgt caaagtcacg ggcgtcttcg ccatcaatgg tgaccagcgg taaatcgcgc
     2641 aaatcgacac ggcccgcttt tgcttcttcc ggcacttctt ctttcagccc cgcaacctgt
     2701 tgctcaacag cctgcggcca gatatacgga atttcatggg tacgcagagc gatatcaacc
     2761 gccatgccgg tgcccatatt gtcgcccagc acttcgacga ttttacccac cgctttggtg
     2821 cggcgagtcg gacgctgagt cagttcgact acgaccacaa agcccatccg cgcgcccatg
     2881 atctgatcgg gcgggattaa gatatcgaag ctcagacggc tatcgtcagg aaccacaaag
     2941 ccgacgcccg cttcggtaaa gtagcgacca acaatctggc tggtttttgg caccagtacg
     3001 cggacaatac gcgcttcacg acgaccttta cggtcagcac ccagcggctg cgccagcacc
     3061 tgatcgccat gaatacaggt tttcatctgc tcgctggaga gatacaaatc atctttacgc
     3121 ccttcaaccc gcagaaagcc gtagccatca cggtggccaa taacggtacc tttcaccagg
     3181 tcgaggcgtt ccggcagcgc atagcactga cgacgagtga agaccagttg accatcgcgc
     3241 tccatcgcgc gcaggcggcg acgcaggcct tcaagctgct cttcgccttc aatgtgcagt
     3301 tctaccgcca gctcatcacg gctggccggt ttttcacgtt tggttaaatg ttcgaggata
     3361 aattcccgac tagggatggg attcgcgtat ttttcagctt cgcgttcctg gaaaggatct
     3421 tgtgacatct cggttcctcc gttgtcatct ctgatgaaga ttttcgtcac tccaccagca
     3481 ataatttata aagcggttga ttctcttcaa ccaaatcggc aagcgtgtag ttatccagtt
     3541 ccgtaagaaa actttgcacg gccttagaaa gcgcctgttt caaccgacag gcaggtgtaa
     3601 tgtggcaaaa ctcactgctg caattcacca gcgataaggg ctccagctcg cgcaccacat
     3661 caccaatacg tatcgcactc gccggtttac ccaggcgaat gccgccattt tttccacgaa
     3721 cagcagtcac gtagccggca cgactaagtt gattgattat tttgaccata tgattacggg
     3781 agacgccgta gacgtcagtc acttcagaaa tactggtcat ccgcccttct ggcaatgacg
     3841 ccatgtagat cagcgcacgt aatccgtaat cagtgaaact cgttaactgc acatcaacct
     3901 caaaagggaa atcgggaaaa aatacattta tattgatgat aaaccagcca caagctgtgt
     3961 cgctaattta tttcagttcg gggaaggaaa aaagcgagga tttaacactg tgtcggatag
     4021 cgtaatggct taaccgacac agcaatgtat cacactgtta gcccggcagg caaaatatct
     4081 gccaggcgta ccagaattac gcgtcgaacg gatcgcgcag aatcatggtt tcagtacgat
     4141 ccggaccggt agagatgata tcgatcggca caccggtcag ctcttcaata cgcttgatgt
     4201 agttcagcgc cgcctgcggc aggccgctac gatctttcac gccgaaagtg gattcagacc
     4261 agcccggcat ggtttcgtaa atcggctcta cacctttcca gtcgtcagct gccagcggag
     4321 tggtagccac ttcgcgacca tccggcatac ggtaagccac gcagagttta acctctttca
     4381 ggccatccag aacgtccagc ttggtcaggc aaaagccaga cagggagttc agctgtaccg
     4441 cacgacgaac ggcaacggtg tccagccagc cggtacgacg acgacgaccc gtagttgcgc
     4501 cgaattcgtt accctgcttg cagaggaact cgccagtttc atcaaacagt tcggtcggga
     4561 acggacctgc acctacacga gttgagtaag ctttgaggat acccagaaca taatcaacat
     4621 aacgcgggcc caggccggaa ccggtcgcca cgccaccagc agtggtgttg gaagaagtta
     4681 cgtacggata agtaccgtgg tcgatatcca gcagcgtacc ctgcgcacct tcaaacatga
     4741 cgaaatcgcc acgctgacgc gcctggtcga gcaggtcaga aacgtcaacc accatagaag
     4801 tcaggatgtc ggcaacagcc atcgtatcat ccagaacttt ctggtaatca accgcttcag
     4861 ctttgtagta gttaaccaac tggaagttgt gatattccat cacttctttc agtttttcag
     4921 cgaaggtttc tttgtcgaaa aggtcgccaa cacgcagacc gcgacgtgct actttatctt
     4981 cataagcagg cccgatacca cgaccggtgg tgccgatcgc tttcgcgcca cgcgctttct
     5041 cacgcgcgtt atccagcgca acgtgataat caaggatcag cggacatgct tcagacagca
     5101 gcagacgctc acgaacgggg atgccacggt cttccagttc tttcatctct ttcatcagcg
     5161 cggccggaga cagcacaaca ccgttaccga tgatgctggt tacattctcg cggagaatac
     5221 ctgatggaat aagatggaga acggtttttt caccgttgat tacgagagta tggcctgcgt
     5281 tgtgaccgcc ctggtagcgt acaacatatt tagcccgttc agtcagaaga tcgacgatct
     5341 tacctttacc ttcgtcaccc cattgggtgc ccagtacgac gacgttgtta cccatttttc
     5401 aaaatcaccg tttgcttaaa aatggattct accatcgctt tttcagagtt acagcacttt
     5461 ttgcactcaa aaatgacctc gatcagtctg ttttttgttc agccaatcgt tttcctcaac
     5521 atgtagtaga ccacaacgcc cgcaaccaca agtccaccgc caaaacgacg taaaatatta
     5581 tcgggcaaat tggtcatcgc agagatcatc ttcttccatg ccttcgggta aagcatcggc
     5641 cctaaacctt ccagtaccaa aaccagggca agcgccagcc agattgtcga attcattcat
     5701 tatccttata gaaaaagaaa accaccgaca tccctgagga tgcggtggct ttattgacct
     5761 gtaccgcagt cgttatatta acgcgttgcg gaagtcggcg tcttcatgta gcggaagaaa
     5821 tcgctatccg ggctcatgac catcacgtcc tgattgccag agaagctgtt ctcataagca
     5881 cgcaggctac ggatgaatgc gtagaagtcc ggatctttac tgaatgcatc agcaaacagt
     5941 ttggctgctt cggcatcgcc ttcaccacgc atgatgcggc cctgacgctc agcttctgcc
     6001 agcgttctgg tcacttcata gtcggcagtc gcgcgcagtt tttccgcttc ttcctgacct
     6061 tgtgaacggt gacgacgcgc taccgcttca cgctcggcgc gcatacggtt gtagatcgct
     6121 tcagacactt cggtcggcag gttgatctgc ttgatacgca catcgacaac ttcaataccc
     6181 agcgccgcca tactgttcgg gttgatgacc ggaactttgc ccttcgtctc agccgttacg
     6241 cgctctgccg cttcggcaat ggcgttatct gccgccgggg tagtaacttc atcttctgta
     6301 cccgcagaac cggagttcag cgcgtcacgt acttcgaggg tcagacgacc acgggaatcg
     6361 gtgacgatgt ctttcacgtc caggcgacca atttcagaac gcagacggtc agagaactta
     6421 cgtttcaaca gcacttccgc ttgcgaaatg tcgccaccac ccgttgccag gtagtaacgg
     6481 ctgaaatcgc tgatgcgcca tttgatgtaa gaatcgacga tcaggtcttt cttctctttg
     6541 gtcacaaagc ggtcggcctg gttgtccatg gtctgaatac gtgcgtcgag cattttcacc
     6601 gtttcaatga acggtatctt gaaatgcaga cccggctcat aaaccagagg tttgttgtca
     6661 tcgtcacgca gtaccttacc aaaacgcagc gtaataccgc gctcaccttc tttgacgaca
     6721 aagacagaca tgtaaagcac taccagcacg atgatgataa tcgcgataac tgacttacgc
     6781 atcgttattc cccctgacgc tggtagtcgt tacgctgcgc gttggcgcgg cgttggtcca
     6841 taatatcgcc ctgactggtg gacgacgtgt tgcttgctcc actggttgtg gaggaagagg
     6901 ctggcggcag acgcagcaga ttgctggcac cgttatcgct cttcgccgca ggggcgttac
     6961 cgcctttcag catctggtct aacggcagaa ccatcaggtt gccaccttta tcgttaacca
     7021 gcactttgcg ggtgttaccc aacacttttt ccatcgtctc gatatacaga cgctcgcgag
     7081 taatttccgg cgcggcttta tattccggca gaagtttagc aaagcgcgcc acttcaccct
     7141 gagcttccag gatggtctgg gccttgtacg cacgcgcctc ttcgaggata cgttgcgcct
     7201 gaccgttcgc acgcggctga acttcgttgg tatacgcttc tgcttcacga atgtattgct
     7261 gttcgttttc acgcgcggca atcgcatcgt caaacgccgc ttttacttct tccggcggac
     7321 gagctgcctg gaagttgacg tccagcagag tgatacccat gtcatacgga cggatagtct
     7381 cttccagttc gcgctgagta tcgctacgaa tcacggtacg accttccgtc agaatgcggt
     7441 ccatggtgta tttaccgata actccacgca gggcgctgtc ggtagcctga cgcaggctgt
     7501 catccgggct ggtcacgcta tacagatatt tttccggatt ggtgacgcgg tactgcacgt
     7561 tcatctcaac gcgcactacg ttctcgtccg acgtcagcat cacaccagaa gcggccagtt
     7621 cacgcacggc ttccacgttc accggtttga cttcgtcgat aaacgtcggt ttccagttca
     7681 gacccggctc aaccagatgg ctgaatttac caaagcgtgt taccacgccg cgttcggctt
     7741 ctttaatggt atagaaacca ctggccgccc agataatgac aatcgctgcc gctgcgatgg
     7801 taacgacacg accgccaagc tgcgggcgcg ggccttgcga tgaactgcca ccgccagatc
     7861 cggtgccttt accgccgccc agaccaccga gctttttgct cagtttgcgg aagatatcat
     7921 ctaaatcagg tggcccttga tcgcgaccgc ctttgtttcc atttccctca gagttgccgc
     7981 caggtttgct gcttccccac gggtcgcggt cttgtccgtt attaccgggc tgattccacg
     8041 ccatgtttgt gctccatatt tgttatgcgg tgatccctgt tgtctttcga cgccagagga
     8101 tatgactcca cgcttcagac gctacgccgt tagatcaggt aatcgatcaa cgccggttct
     8161 tgtttacaga ggcgacgcca gtcaacgatc ggcatacgaa cttgcagact tacgctgccg
     8221 tcctcctcca tccactcttt ttctattgcc tgaagctgat aaaaacgact tctcagacgt
     8281 ccttcctgcg gcggcaaacg caatgtatgc tgcgccacct cgccggaaag ccgctccgtc
     8341 aaagcctgaa aaagctgtgg tatccccgct ccggtctgtg cggaaagcca gacacggatc
     8401 ggtttgttct cttcgtcccg atcaatacgc ggttcgaaat cttccagcat atcgatcttg
     8461 ttcatcacca gcagggttgg gatctcgtga gcgtcgatct cttcaagaac cgtattcacc
     8521 gcttcgatgt tttcttgtac acgtacatcc gccgcatcaa tgacgtgcag cagtaatgtg
     8581 gcttgccgcg tctcttgtaa cgtggcttta aatgctgcca ccagatcgtg cggcaggtgg
     8641 cgaataaacc ctacggtatc tgcaagtacg gtttcaccga catccgcgac gtcaatacgc
     8701 cgcaacgtcg ggtcgagggt ggcaaacaac tggtctgccg cgtagacccg cgcttcggtg
     8761 atgcgattga aaagggtaga tttaccggcg ttggtatatc ccaccagcga aacagtagga
     8821 acgtcggctt tgatacgcga ttgccgcccc tgctcacgct gcttttcaac tctttctagg
     8881 cgcgactgta tctgcacgat gcgattacgc aacaaacgac ggtcggtttc gagctgggtt
     8941 tcacccggac cacgcaaacc tatcccgcct ttctgtcttt caaggtgggt ccagccacgc
     9001 accaggcgcg tagccagatg gcgcagctgc gctagctcaa cctgcaactt accctcatgg
     9061 gtacgcgcac gttgggcgaa aatatctaaa ataaggccgg tgcggtcgat aacacgacac
     9121 tcgcacaaac gctccaggtt acgctcttgc gccgggctca gggcatggtc aaaaagaacg
     9181 accgaagcac ccgtcgcttt gacagcttcc gcaatttcaa ctgctttacc ttcacctaca
     9241 aaatactttg ggtgcggcgc tttacggcta ccggtaatca cctgcaatgc ttcgacaccg
     9301 gcggaagaga ccagagattc aaactcctgg aggtcttcca tatctttgtc ttgcgtaaaa
     9361 tagatgtgta ccagtaccgc ctgctcacca gcatcataac ggtcaaacaa gcgtataacc
     9421 ctctaaatag atcagcgggg aacgcaggat cgctggctcc ccgtgtaaaa aaaacagccc
     9481 gaaaccttat tcggtttctt cgctgtcctg ttgcgcggaa gtattctgcg cgcagctacc
     9541 atgatggtag ttactgctgg taccgccacc ggcgttgtta ctgtgatgag aaaccgggcg
     9601 agacgggaca acagtagaaa tcgcgtgctt gtaaaccatc tggctgaccg tgtttttcaa
     9661 caggatcacg aactgatcaa aagactcgat ttgcccttgc agcttaatac cattcaccaa
     9721 ataaatagaa actggaacac gttcccgacg cagtgcgttc aggaacggat cttgtaaaga
     9781 ttgcccctta gccattctct cttttcctta tatgcttatt tgtactttga acctttcgat
     9841 tctgaaaaaa ttgcgcacga tacgtctcaa ttgtacacat tcagcctgcg atagcaccaa
     9901 caacctgtaa tacttcgtca cgcgcctgtt ctggtttttc actgtcaagc cagtgaaccc
     9961 cttcccaacc acgcagccag gttatctgcc gcttcgccaa ctgtctcgtg gcgcaaacac
    10021 ctcgataaac catttcatcg tatgagattt cgccttcaag gtaagaccac atctggcgat
    10081 aacccacgca acgaatggaa ggcaagtccg tatgcaaatc tcctcgggca aaaagcgccc
    10141 ggacttctgc ttcaaaacct gaagccaaca tctgatgaaa acgctgctca atgcgttgat
    10201 ggagcagttc acggctcgcc ggggcgatgg cgaactgatg cacctgatac ggtagagcgt
    10261 ctcctgacgt ttgcgtcagt tccgttaaag ttttacccga aatgaaaaaa acttccagtg
    10321 cccgggaaag cctttgtgga tcatttggat gaatccttgc tgccgcaacc ggatctacct
    10381 cctgaagttg acgatgcaat gactcccaac cttgctctgc cgcctgttgc tcaattctgg
    10441 cccgcacttc cgggtctgcc gacggtagcg gcgacaaccc ttccagcaat gccttgaaat
    10501 acaacatcgt accgcccact aacagtggga tccgccccgc cgcggtgata tcggccattt
    10561 ccgccagcgc atcgcggcga aaatcagcag ccgagtaagc ctgcgacgga tcgcgaatat
    10621 ccagcaatcg gtgcggcgcg gcgagtaact cttcagcgtt cggcttcgcc gtcccgatat
    10681 ccatcccttt gtaaataagg gcagaatcaa cgcttatcaa ctctactggt aaaattttac
    10741 gcagctcaat ggctaacgcc gttttaccgg aggccgtcgg ccccatcaaa aaaatcgcct
    10801 taggcaggct cgccttactg atatcactca tctttcaggg cttttatcgc cggatgtaaa
    10861 tcaacagatt gtaacagacc acccggcggc gttttcacaa gttgcggaca taaccgttcc
    10921 acgtccgcca gcagggttat ggcctgtgcc attgaccact gcgcatgttc gctcatcaga
    10981 tttcgtgcaa tccactgcgc aatattgcca ggttcgaata cggactgctt cgccaggtag
    11041 cctatcagtt caggaatcaa gatttgtaaa ttttgttggc gtaagggtaa aggcacggcc
    11101 ctgatggtca catgctgtgc atctgactgg aaatcaatac ccaattccgc cagggcagac
    11161 tgcgcttttt ctaatgccga tttttcttcg ccagaaactt ttagccgcaa cggaataagc
    11221 agcggctgag cgcaaactgg cgcttcaccc ggcgtcagtt gtacctgacg cagccaacgt
    11281 tctgccactg gcaaggctaa aagtgaaatg ttgccgtcgc gctccagcaa cgcacagtcg
    11341 gaatggacga tagtcagtac ccgaccaaaa ctctgactgt tcgccgcaag tgcaggttcc
    11401 tgcggttccg gcgcttttaa tttttgcatc ggcgcgggcg tttgcaaaag ctggcgatac
    11461 acttcacctt gctgtttctg gtagcctggc tgcgcattcg gccagggggc agccggacga
    11521 ctgcctgatg ctggcgcagg agtgtagcgc ggagctaccg gctcacgagc tgccggttct
    11581 gcaaagtgat tgcgccccgc cgccacgcgg ttttccggaa tggaacgcgg tgcaggttgg
    11641 ggttcatcgt ccagcggtag cggcgtttcc agttgctgtt gtagcacgct cagcacgccc
    11701 tgatagataa aatcatgcac cagacgcgac tgatggaaac gcacttcgtg tttggcgggg
    11761 tgcacgttga cgtccacctg atgcggatcg atctccagat acagcacaaa tgccggttgc
    11821 tgatcggccc ccagtttgtc ttcgcaggcc tggcggatcg cgtgattgat caggcgatcg
    11881 cgcatcatgc gaccgtttac gtagcaatac tgaatttctg ccagtgcggg cgtggtgtga
    11941 tttggatcgg ccacccagcc gcgtagcgtg agatcgccgt gttgccattc aatcgccagc
    12001 gcttgttcaa gaaaagcggt gccgcaaatc gcgcctaagc gccgttcttt ttgcccgcct
    12061 tccggcactg cgcggtactg acgcacaatt ttaccgttat gcgacaggtt gatcgtgacg
    12121 tcgaaacgcg ccagcgcaat gcggcggatg atctcatcaa tgtggttaaa ttcggttttc
    12181 tcggtgcgca ggaatttgcg ccgcgccggg gtgttgtaga acagatccag cacctccagc
    12241 gtcgtcccca caggatgcgc cgccggtttt accgtcacgt tcatatcgcg cccttcggca
    12301 taggcctgcc aggcttcctg ctgttctgcg gtgcgtgaag tgagcgtcag gcgggaaacc
    12361 gaactgatac tcgccagcgc ctcaccgcga aagcccaggc tgataatggc ttcgagatcg
    12421 tccagagagg cgattttact ggtggcatga cgagccagcg ccagcgccag ctcatctttt
    12481 ttgataccgc agccgttatc acgaatgcgg ataagtttcg ccccaccgcg ttcgatatca
    12541 atatcgatac gcgtcgcacc tgcatcgagg ctgttttcca ccagttcttt gactaccgac
    12601 gcaggtcgct cgaccacctc acctgcggca atctggttcg ccagttgtgg cggtaagacc
    12661 tgaattggca tcaatcgtcc ttagtttggc agcgtgcgat ctggcgtcgt caccgtactg
    12721 gcagtttgtg ccgttgcccc ctgcggcgca gattgcatcg gatgcgcaag gaaataattg
    12781 cgcaggcctt tgtaaatggc ttctgccagc tgttgttggt aatcgtcgct cgccagcaaa
    12841 cgttcttcgc tgttgttgct gataaaaccg gtttcgacca gtactgacgg gatatccggc
    12901 gaacgcagaa cgccgagact cgcgtgttct ggtcggcgtt tatgtatttc gccaatgcgt
    12961 tgcaactgac tgatcatact ggtcgctaca tcatacccta cccgctggga atgaccgaac
    13021 tgtaaatcca gcaccgcctg gcttaaatag gggtcagact gactgttcgc cagcacatcg
    13081 cccgccccac ccagcagctc cgactgtttc tcgtgctgct ccagccagct ggccatttcg
    13141 ctgttggcgc gacggttaga gagcacccat acggaagcgc cagtcgcact gcggttcggt
    13201 gcggcatcag cgtgaatcga cacgaggaaa ttggcgtttt gcttacgtgc cacatcgctg
    13261 cgccccatca ccgagataaa gtaatccccg tcacgggtta aaacgccttt aaacatcgga
    13321 tcgtcattga gcaaagtgcg caatttacgc gcgatggcga tggtgacatt tttctcccgc
    13381 gtaccaccgg ggccgatagc gccagggtcc tgaccgccgt gtccggcatc aatagcgatg
    13441 ataattttat cgccagtgtt agccgtcgcg cgcgctgccg gacgcgttac cgtattactg
    13501 ctgataacac ccgtagtgcg gttactttcc gttttaaacg gattgcgcgc cggttcgctg
    13561 acgcgcggtg cgacaaccgc aggcgtttca acgcgtttcg caaccacggg cggtggagga
    13621 ggcggtggcg gcacatcggc gttaatcgta aagacgacag tgtaattgct gccattctgc
    13681 cgcttcaccg cttcggtttt accgttttcg gtaagatcga ccaccagccg tagcgtttgt
    13741 gcatctttag gcgttccaga gcgaatcgcc ttcaccagat tattgccgct gaacaacaac
    13801 ggcagtccct gaatcacgcc cgtttgtttg atatcgagcg ccacggtgcg tttgctttga
    13861 tggctaaacg cataatcagg atcgccaata aaactcaacg ttatccgcgc ctgttgatta
    13921 ccgttagaaa cctgaatatc agagagcgtc gcggcaccca ccggcgtgca cagcagcagc
    13981 agcgtcgcta ccaaccaatt tctgatgcga tacatcatcc cgccaccttt caaaggttaa
    14041 ccggctaaac gcgccagcaa caattcaccc gcagaggaaa ccgcactcac gcgcgcctca
    14101 cggccttgtg cctgataatc aatgtgtatt tcgacatccg ggtcaggaag aacacctgta
    14161 ccttgttgtg gccactccac caggcagatg gcatcgttgg caaaataatc gcggatcccc
    14221 ataaactcca gctcctcggg atcggcaagg cggtacaaat caaagtgata aaccattaaa
    14281 ttgtcgagcg tatagggttc gaccagcgta taagtggggc ttttgacatt accctgatga
    14341 cccagagctt gtaaaaaacc ccggctaaag gtggttttac ctgcgcctaa atcgccatac
    14401 agatagatta cggttgcgcc atcgcaggct ttcgctaccc gctcgcccag gtctaatgtt
    14461 gcctgctcat cagggagcgg aattactcga ttcatcatgg tttttatcag tcacttccgg
    14521 gttaacaata cgctgtagcg tggaaaagag atcggttgcc agcatcccgc gcgttccaaa
    14581 acgcgccgcc agtacgtcag ctgccgcacc gtgcgcgaca cagcccgcac aggcggcatc
    14641 atacggcgac agtttttgcc caagcaatgc gccaataata ccagagagca catcgcccat
    14701 gccgccgctc gccatgcctg catttccggc atcaataatg cctaaagcgt caggatgggc
    14761 ggcgaccacg gttccggcac ctttcagcac cgctacgccg ccataacgtt gtaccagacg
    14821 tttggcgcaa tgtaagcggt cactttcaat ttcagcgacg gaacagccta acaaccgtgc
    14881 ggcctcgcca ggatgcggcg tgatcacgcg attgtgacgc ttatcgggat tgattgccag
    14941 caggttcaat gcatcggcat cccacaacat cggtttgcga aaattctcaa ctttttgcag
    15001 tgcttttttc ccccactctt gctggcccag accgggacca atcaccacca catcggccca
    15061 ttccaggctt tcggtaagag agtccatcgt cagttcatgc accatcaatt ccggtcgtgc
    15121 agtcagcagc ggcgcaatgt tttcactgcg ggtcagtact cggactaaac cagcaccagc
    15181 acgcagcgcc gcttcccccg tcatacgaat agcccccgcc gtgccgtgat cgccaccgat
    15241 aatcaccagc cgcccgtgat cgcctttatg cgaagtcggg cgacgcggtt tcagccagtg
    15301 agaaagttgt tctgccgaaa accgctgaat tttcgtctcc tgacctgcca gccagctatc
    15361 cagccccagt gagtcaaaat gcagttgtcc tgtaacatcc cgcgcttttc cagtaagcaa
    15421 gcctggtttc agcgcaataa aagtgatggt gtgatcggcg ttgatcactg cgcctggcgt
    15481 agcgccggtt tcagccagca ggccggaagg gatatcaacc gccacaatcg gcgcaggatg
    15541 ggaattagcg tggtcgatta actggctaat ggattcgcgg ggcgcttgct gcaagccggt
    15601 accgagcagc gcatcaacaa tcagatctac cgattcgggc cagacaatat tcgaagcatg
    15661 gatctcgcca cccgcgttta accatgcttc gcgtgccagc gcggcctctt ccggcaacgg
    15721 tttgtcgctc tcctgggcca acaacgtgac ctcaatgccg accgctttgg ccagtcgcgc
    15781 gaccacgtag ccatcgccgc cgttattacc atgaccgcac agcaccagcc agtggcgggc
    15841 gtcaggatac gccgaacgac acacctggaa tgcggcctcg ccagcgcgaa gcatcagctc
    15901 atagagtgtg agccccagca catctgccgc ctcgcgttct ccgcggcgga tatcgtcggc
    15961 gtaccagacg gtgtgtggta tacttacggg gtttttcttc attgtatggt ccgtcatgtc
    16021 agagcccctc gatctcaatc agttagcgca aaaaattaaa cagtgggggc tggaactggg
    16081 ctttcagcag gtaggtatta ccgataccga tctcagcgag tccgagccca aactgcaagc
    16141 atggctggac aaacaatacc acggcgaaat ggactggatg gcacgtcacg gtatgctgcg
    16201 cgctcgcccc catgagttat taccaggtac gctgcgtgta atcagcgtgc ggatgaatta
    16261 ccttcctgct aacgccgcat ttgccagcac gctgaaaaac cccaaactcg gctatgttag
    16321 ccgttatgcg ctgggccgtg actatcacaa acttctgcgc aaccgactca aaaagctggg
    16381 cgagatgatt cagcaacatt gtgtttcgct gaattttaga ccgtttgtcg attctgcgcc
    16441 tattctcgag cgcccgttag ctgaaaaagc tgggctcggc tggacaggta agcactcact
    16501 tatcctcaat cgcgaggccg gttctttctt ctttttaggc gaattgctgg tcgatattcc
    16561 gctgcccgtg gatcaaccag tcgaggaagg atgcggtaaa tgcatagcct gtatgacgat
    16621 ttgcccgacc ggtgccatcg tcgagccata taccgtcgat gctcgccgct gtatctctta
    16681 tctcaccatc gaacttgaag gggcgatccc ggaagagttg cgaccgttaa tgggaaaccg
    16741 tatttacggt tgcgatgact gccagcttat ctgcccgtgg aatcgctatt cgcaactcac
    16801 tacagaagac gatttcagcc cgcgtaagcc gctacacgca caggaactca ttgagttatt
    16861 tgcctggagc gaagagaagt ttttaaaagt cacggaagga tcggcgattc gccgtatagg
    16921 tcacctgcgc tggctgcgta atatcgccgt ggcattaggc aatgcgccct gggatgaaac
    16981 gattctggcg gcgctggaga gtcgcaaagg tgagcaccca cttcttgatg agcacatagc
    17041 gtgggcgatt gcgcagcaaa tcgagagacg aaatgcgtgc atagtcgaag tgcaattgcc
    17101 gaaaaaacag cgtctggttc gggtgattga aaaagggtta ccgcgtgacg cctgattcat
    17161 ccacagcttg tgaataaaaa taaaaacgca ttgcaattca agacgcataa aatcgtcaag
    17221 tgatcgactc gacaaaacgg aatcttgatt tttaacttat ttatcaatag gttaaaaaaa
    17281 taccattcac cgtgcgaagt ttctttgaaa ttaagattac agcctgagga tgtggataac
    17341 tctgtttaca agagtttgtc agagacaaca aaaaccagca ataacgcatc gcgtcgctgt
    17401 ggatatttta ttgagagaag aatttggagc gggaaacgag actcgaactc gcgaccccga
    17461 ccttggcaag gtcgtgctct accaactgag ctattcccgc ttgggtggtc tgtgccttac
    17521 agcactttca aattttggag cgggaaacga gactcgaact cgcgaccccg accttggcaa
    17581 ggtcgtgctc taccaactga gctattcccg cttaatcttc gtctttcgag ccgctactgc
    17641 cttggctgcc ttcactcacc ttagtcacat acttttgtaa gttcccaagg attcgttcag
    17701 ttgccgcctt gtcgcaattc gaaatactgc gattgggtgg tctgtgcttt gcagcacttt
    17761 tcaaattttg gagcgggaaa cgagactcga actcgcgacc ccgaccttgg caaggtcgtg
    17821 ctctaccaac tgagctattc ccgcgtacta cttaattttg ctttcgtaat tttcgcattt
    17881 ctgcgtcgtt acgggaggcg cattatacga gaaatctttc cctctgcaac cccccaaaaa
    17941 agtttttttt gcgtttttat ctcaagtgct gaaattatca gcaagacgtt caatttagcg
    18001 tcaaaatcac gccgccgcaa gtggattccg gcgcttacgt gaccaggaaa aattttacag
    18061 cttgataaaa tgctcgcggt agtaagccag ctccgccacc gattcacgga tatcatccat
    18121 tgcctgatgc gtcccctgct tggtaaaacc atccagaatt tccggcttcc agcggcgcgc
    18181 cagctctttc agggtgctga catcgagata acggtagtgg aagtaggctt ccagctccgg
    18241 catgtattta aacaggaaac gacggtcctg tccgatgctg ttaccgcaaa tcggcgattt
    18301 tcccgcaggc acccactgtt ttaagaattc gagcgttgcc agttcagctt cccgatcgcc
    18361 catcgtgctc gctttcacgc gctctaccag cccgctggcg gtatgggtgc gcacgttcca
    18421 gtcatccatc agcgccagct gttcatcgga ctgatgtact gcaatggtcg gcccttctgc
    18481 cagaatattc agattggcat cggtcaccag cgtggcaatc tcaataatgc gatcgcgctc
    18541 gggatccaga ccggtcatct caagatcgat ccaaatcagg ttgttttcat tggcactcat
    18601 gctattttcc acccattcac agtgactata ttgatccaca attgcgtgta tcatagatgt
    18661 tttgcccatc aggggcgacc aggagtcagt acgattgagt aaaaataaac tctccaaagg
    18721 ccagcagcgc cgcgtgaacg ccaatcacca gcgtcgtctt aaaacgtcta aggagaagcc
    18781 cgactacgac gacaatctgt ttggcgagcc tgatgaaggt atcgtcatca gccgctttgg
    18841 tatgcacgct gatgtggaat ccgccgatgg cgacgttcac cgctgcaata ttcgccgtac
    18901 catccgttcg ctggtaaccg gcgaccgcgt agtctggcgt ccgggtaaac cggcggcgga
    18961 aggcgtaaat gtcaaaggga tcgtggaagc ggtgcatgag cgtacctcgg tgttgacgcg
    19021 tccggatttc tacgacggcg taaaacctat tgccgccaac atcgaccaga ttgtcattgt
    19081 ctccgccatt ttgccggagc tgtcgctcaa tattatcgac cgttacctag tggcctgcga
    19141 aaccttgcag attgagccga ttattgtgct caacaagata gacctgctgg acgacgaagg
    19201 catggcgttc gtcaacgagc agatggatat ctaccgcaat atcggttatc gcgtattgat
    19261 ggtttccagc catactcagg atgggctaaa accgctggaa gaggcgttga ccgggcgcat
    19321 cagcattttt gccgggcagt ctggcgtcgg caaatccagc ctgctgaacg cgttgctggg
    19381 gctgcaaaaa gagatcctga ccaacgatgt ctctgacaac tcggggcttg gtcagcacac
    19441 cacgaccgcc gctcggctgt atcacttccc gcacggcggt gatgtgattg attcccctgg
    19501 tgtgcgtgag ttcggcctct ggcacctgga gccggaacaa atcactcagg gctttgtcga
    19561 attccatgac tatttaggtc tgtgtaaata tcgcgattgc aaacacgata ccgatccggg
    19621 ctgcgctatc cgggaagcgg ttgaggaagg gaaaatcgcg gaaacccgtt tcgaaaacta
    19681 tcaccgtatt ctggaaagca tggcgcaggt aaaaacgcgt aaaaactttt ctgatacgga
    19741 tgactgacaa ttaagctaac cgtcgttaga atcgtcccct ttttttcagg atccggcatg
    19801 taggccgaat caggaacgac aaaacaatgg cctggaggct accttgttaa attcatttaa
    19861 actttcgcta cagtacattc tgccgaaact atggcttact cgcctggcgg gttggggcgc
    19921 aagcaagcgg gcaggatggt tgacaaaact ggttatcgat ctgttcgtta aatactacaa
    19981 ggtcgacatg aaagaggcgc aaaagccgga caccgccagc taccgcacct ttaacgaatt
    20041 ctttgtccgt ccgctgcgtg acgaagtacg cccaatcgat accgatccga atgtactggt
    20101 catgcctgcc gatggcgtta tcagccagtt gggtaaaatc gaagaagata aaatcctgca
    20161 agccaaaggc cacaactaca gcctcgaagc cctgctggca ggcaactatc tgatggcgga
    20221 cctgttccgc aacggtacgt ttgtgaccac ttacctctcc ccgcgtgact accaccgcgt
    20281 acacatgccg tgcaacggta ttctgcgtga gatgatctac gtgccgggcg atctcttctc
    20341 cgttaaccat ctcacggctc agaacgtgcc gaatctgttt gcccgtaacg aacgcgtgat
    20401 ttgccttttc gataccgaat ttggcccaat ggcgcagatt ctggtcggag cgacgattgt
    20461 tggcagcatt gagacggtct gggcgggcac cattacgccg ccgcgcgaag gtatcatcaa
    20521 gcgctggacc tggcctgccg gggaaaacga cggttctgtg gcactgctga aaggccagga
    20581 aatgggtcgc tttaaactcg gttccaccgt tatcaacctg tttgcaccgg gtaaagtgaa
    20641 tctggttgag caactggaaa gcctgtctgt cacgaaaatt tgccagccgc tggcagtatc
    20701 taccgaaacc tttgttacgc cagacgctga acctgccccg cttcctgctg aagagatcga
    20761 agcagaacac gacgccagcc cattggttga cgacaaaaaa gaccaggtct aatccatcaa
    20821 aggaaacgct gacgtgcgcc tgattatcac ttttctgatg gcctggtgcc tcagttgggg
    20881 ggcgtacgcc gcgacggccc ccgatagcaa acaaatcact caggaactgg agcaggcaaa
    20941 agcggcgaaa cccgcacagc cggaagtcgt agaggcgctt cagtctgcct taaatgcgct
    21001 tgaggaacga aaaggttccc ttgagcgcat caaacaatat cagcaagtta tcgataatta
    21061 tccgaaactc tccgctactc tgcgcgcaca attaaacaac atgcgtgaca agccgcgcag
    21121 cgtgtcgccg ggaatgtcta ccgacgcgct gaatcaggaa attctccagg tcagcagcca
    21181 gttgctggat aaaagccgtc aggcccagca agagcaggag cgcgcccgcg agattgccga
    21241 ttcgctgaat caactgccgc aacagcaaac cgacgcccgc cgtcagttaa atgagatcga
    21301 gcgccgcctg ggaacgctta ccggcaatac tccgctcaat caggcacaaa atttcgcgtt
    21361 gcagtctgac tctgcacgtc ttaaggcgct cgttgatgaa ctggagctgg cgcagctgtc
    21421 tgccaataac cgccaagaat tagcgcgctt acgctcagag cttgcggaaa aagagagcca
    21481 gcaactggat gcgtatttgc aggccttacg taatcagttg aatagccaac gtcagcttga
    21541 ggcggagcgg gcgctggaaa gtaccgaatt actggcagaa aacagtgccg atttgccgaa
    21601 agatatcgtc gcgcaattca aaattaaccg cgaactatcg gcagcgctga atcaacaggc
    21661 gcagcggatg gatctcgttg cctcgcaaca gcgtcaggct gccagccaga cgttacaggt
    21721 ccggcaggcg ttgaatacgc tgcgtgaaca gtcgcaatgg ctgggatcgt ccaatctgct
    21781 cggtgaagcg ttgcgagcgc aggtggcacg gctaccagaa atgccaaaac cacaacagct
    21841 tgataccgaa atggcgcagt tgcgtgtgca acggttacgt tatgaggatc tgcttaataa
    21901 acagccgctg ctacggcaaa tccatcaggc cgacggtcag ccgctgaccg ccgagcaaaa
    21961 ccgtattctg gaagcacaac tgcgcactca gcgtgagttg ctgaactcat tgttgcaggg
    22021 tggcgacacg ctactattgg aactgaccaa gctgaaagtc tccaacgggc aactggagga
    22081 tgcgctgaaa gaggtgaacg aagcaacgca ccgctatctg ttctggacct ctgacgtgcg
    22141 tccgatgacc atcgcctggc cgctggaaat cgcccaggat ctgcgtcgtc tcatttcgct
    22201 ggacaccttc agtcagttgg gcaaagccag tgtgatgatg ctgaccagca aagagacaat
    22261 tttgccgctg tttggcgcgt tgattctggt cggttgcagt atttactcgc gccgctattt
    22321 cacccgtttt cttgaacgtt cggcggcgaa agttggcaaa gtgactcagg atcacttctg
    22381 gctgacgttg cgcactcttt tctggtcgat tctcgtcgcg tcaccgttac cggtgctgtg
    22441 gatgacgctg ggttacggct tgcgcgaggc gtggccttat ccgctggcgg tcgcgattgg
    22501 cgatggtgta acggccaccg tgccgctgct gtgggtagtg atgatttgcg ccacctttgc
    22561 ccgcccgaac ggcttgttta tcgctcattt tggctggccg cgcgaacgcg tatctcgtgg
    22621 gatgcgctac tacctgatga gcatcgggct tattgtgccg ctgattatgg cgctgatgat
    22681 gttcgataac ctcgacgacc gtgaattctc cggttcgctg ggaaggcttt gctttatcct
    22741 catttgcggt gcgctggcgg tggtcaccct cagcctgaaa aaggccggga ttccgctgta
    22801 tctcaacaaa gaaggcagcg gcgacaacat taccaaccat atgctgtgga acatgatgat
    22861 tggcgcgccg ttggttgcca ttctggcgtc ggctgtgggt tatctggcaa cggcacaggc
    22921 gctgttagcg aggcttgaaa cctcggttgc catctggttc ctgctactgg tggtttatca
    22981 cgttatccgc cgctggatgc tgatccagcg ccgcaggctg gcgtttgacc gggcgaagca
    23041 tcgccgggca gagatgttag cgcaacgtgc gcgtggcgaa gaggaagcgc atcatcacag
    23101 tagcccggaa ggagcaattg aagtcgatga aagcgaagtc gatctcgatg ccatcagtgc
    23161 gcaatccttg cggctggtgc gctcaatttt gatgttgatc gccctgcttt ctgtcattgt
    23221 gctgtggtca gaaatccatt ccgctttcgg cttcctcgaa aatatttcgc tgtgggatgt
    23281 cacctccacg gtacagggcg tagaaagtct ggagccaatt accctcggtg cggtgctgat
    23341 tgccattctg gtgtttatca tcaccacgca gctggtgcgc aacctgcccg cgctgctgga
    23401 actggcgatt ttgcagcacc tggatttaac gccgggtacg ggctacgcca tcaccaccat
    23461 caccaaatat ctgctgatgc tgattggcgg gctggtcggc ttctcgatga ttggtattga
    23521 gtggtcgaaa ttgcagtggc tggtcgccgc gctcggtgtt ggtctcggtt ttggtttgca
    23581 ggaaattttc gccaacttta tctctggtct gattatcctg ttcgaaaaac cgattcgcat
    23641 tggcgatacg gtgacaattc gcgatctcac cggtagcgtg acgaaaatta acacccgcgc
    23701 caccaccatc agcgactggg accgtaaaga gataatcgtg ccgaacaagg cgtttattac
    23761 cgagcagttt atcaactggt cgctctctga ctcggtcacg cgcgtagtgt tgacgattcc
    23821 ggcccctgct gatgccaaca gtgaagaagt gacggaaatc ctgctcaccg cagcgcgtcg
    23881 ctgctcgctg gtgatcgaca acccggcacc ggaagtcttc ctggtggatc tgcaacaagg
    23941 gattcagatt ttcgagctgc gtatttatgc cgctgagatg ggtcaccgta tgccgctacg
    24001 ccatgagatc caccagctga ttctggctgg cttccatgcc cacggtatcg atatgccatt
    24061 cccgcccttc cagatgcgtc tggaaagcct caacggtaaa caaacgggga gaacgctgac
    24121 gtctgcgggc aaaggtcgtc aggcgggaag tttgtaatgc atgaagctgc caaatgatgt
    24181 caacgtggct catttagcag cttaacatca tcttcagtta tcaccggccc tggagagtat
    24241 catccatacg cattacttcc tgacaactca tcgccggaga gacgatgagt tgcaggattt
    24301 catatgatca gtcgagagct aactcaggtt tattcgccat gcgactgcaa tagttctggt
    24361 aaatcgccat cgctaacagc gagaagaaga cagggccgcc aatcatccac aatgtgctgt
    24421 cccagtctcc ggcttcaacc acaggctgaa taatggtgaa gacgttcgca aatgtcacca
    24481 ccagtacgac aaccaccatc gcaatcattg ccgacatacg cgttttgaaa atcacaaatg
    24541 gtctgtcgag atcctgacgt gctttaaaga atgggaaagc cagcgcgagg aacaggtaag
    24601 gaagcgtcat agacacgttc gccatcagcg tcagcttgtt aaagaacgcg gatgcggtac
    24661 cgccaccaaa cgaaaccagc aggatgaaga cagtaaccaa cccgcactgc atccacatgg
    24721 cgatagacgg catccccatc gcattcaggc gcgtcatcgg ttccggccac aacgctttcg
    24781 gcgtcccctg gatgatggct ttcagcggtg aatagcaaag cgtaaagaac gcaccggtat
    24841 aagcgaggaa catcgaaagt ccggtaatac gcgcaaacca tacgcccagc gacaatgacg
    24901 cttcaggtga caaatgcagt gcgttaccca gcgtcacccc gaggctcttc atcagcacat
    24961 aggtaatatt gccaaggtta acggaaccat tacttaatac ctgctgccag tttgtgctga
    25021 cgccccataa aaatattgcc agcgaataac cgattgaaat aacaatagcg gcaaaaacaa
    25081 tacctttggc aaagttcttt tctggatttt ccgttttatc gaccagacca ccaaccgctt
    25141 caattccgcc ataggcaaaa atagcaaata ccacaaacga tagcattgcc agaccggact
    25201 gataacccgg gttcggtgat gcaaggaaat taatatcctg cgcgaaatgc ccaccattta
    25261 ataacaaaat agtaatgctt actaacagca atactaaatt cagacacatt actgcaatac
    25321 cgcccaccgc agtaatgcgg gcaattttat taatcccctt tgaagcaacg acggtgacca
    25381 gaatcatcca tgctactgcc agcagaccaa ccacctgcgt aggctccagt ccggcaatac
    25441 gccagtgctg ggtcatgtcg ctaccataga ggaatgttga gaacggtacc caaactttcg
    25501 cggaggtact caccatccag atgatataag aggaaaacca cataaacgta ccaatgaagg
    25561 caaaacgtgg tccgacacta ttattcatcc aggaatagat accaccttct tctttgcgat
    25621 aagcagctcc catttcagcc atcatta
//
LOCUS       DADMKF010000050        25104 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.051, whole genome shotgun sequence.
ACCESSION   DADMKF010000050 DADMKF010000000
VERSION     DADMKF010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25104
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.051"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            194..382
                     /locus_tag="JNC24_001873"
     CDS             194..382
                     /locus_tag="JNC24_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9158625.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            394..1146
                     /gene="bcsQ"
                     /locus_tag="JNC24_001874"
     CDS             394..1146
                     /gene="bcsQ"
                     /locus_tag="JNC24_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HAY9158626.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDACPDNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRL
                     SDICSGLQQLKASGRYQWILIDLPRDASQITHQLLSLCDHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINDFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEY
                     RSDALAAEEILTLANWCLLNYSGLKTPVGSAS"
     gene            1143..1307
                     /locus_tag="JNC24_001875"
     CDS             1143..1307
                     /locus_tag="JNC24_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000025864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase"
                     /protein_id="HAY9158627.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPR"
     gene            1500..3761
                     /gene="bcsA"
                     /locus_tag="JNC24_001876"
     CDS             1500..3761
                     /gene="bcsA"
                     /locus_tag="JNC24_001876"
                     /EC_number="2.4.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
                     /protein_id="HAY9158628.1"
                     /translation="MNELPERVSHKTQHLDEKKELGHLSAGARRLILGIIVTFSLILA
                     LICVTQPFNPLAQFIFLMLLWGVALIVRRMPGRFSALMLIVLSLTVSCRYIWWRYTST
                     LNWDDPVSLVCGLILLFAETYAWIVLVLGYFQVVWPLNRQPVPLPKDMSLWPSVDIFV
                     PTYNENLNVVKNTIYASLGIDWPKDKLNIWILDDGGREEFRQFAQNVGVKYIARTTHE
                     HAKAGNINNALKYAKGEFVSIFDCDHVPTRSFLQMTMGWFLKEKQLAMMQTPHHFFSP
                     DPFERNLGRFRKTPNEGTLFYGLVQDGNDMWDATFFCGSYAVIRRKPLDEIGGIAVET
                     VTEDAHTSLRLHRRGYTSAYMRIPQAAGLATESLSAHIGQRIRWARGMVQIFRLDNPL
                     TGKGLKFAQRLCYVNAMFHFLSGIPRLIFLTAPLAFLLLHAYIIYAPALMIALFVLPH
                     MIHASLTNSKIQGKYRHSFWSEIYETVLAWYIAPPTLVALINPHKGKFNVTAKGGLVE
                     EEYVDWVISRPYIFLVLLNLVGVAVGIWRYFYGPPTEMLTVVVSMVWVFYNLIVLGGA
                     VAVSVESKQVRRSHRVEMTMPAAIAREDGHLFSCTVQDFSDGGLGIKINGQAQILEGQ
                     KVNLLLKRGQQEYVFPTQVARVMGNEVGLKLMPLTTQQHIDFVQCTFARADTWALWQD
                     SYPEDKPLESLLDILKLGFRGYRHLAEFAPSSVKGIFRVLTSLVSWVVSFIPRHPERS
                     ETAQPSDQALAQQ"
     gene            3772..6111
                     /gene="bcsB"
                     /locus_tag="JNC24_001877"
     CDS             3772..6111
                     /gene="bcsB"
                     /locus_tag="JNC24_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HAY9158629.1"
                     /translation="MKRKLFWICAVAMRMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMPGVQGADAPVVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNLEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKSDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKTPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLAAISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            6118..7224
                     /gene="bcsZ"
                     /locus_tag="JNC24_001878"
     CDS             6118..7224
                     /gene="bcsZ"
                     /locus_tag="JNC24_001878"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase"
                     /protein_id="HAY9158630.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVIA
                     PSDARKITTSEGQSYGMFFALAANDRVAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYLPPTLAQYFTRFGAPWTTLRETNQRLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPIGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDAYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            7206..10698
                     /gene="bcsC"
                     /locus_tag="JNC24_001879"
                     /pseudo
     CDS             7206..10698
                     /gene="bcsC"
                     /locus_tag="JNC24_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsC"
     gene            10780..12768
                     /gene="hmsP"
                     /locus_tag="JNC24_001880"
     CDS             10780..12768
                     /gene="hmsP"
                     /locus_tag="JNC24_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HAY9158631.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPIMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFAHPLPIEIFEESYLEEK"
     gene            12951..14237
                     /locus_tag="JNC24_001881"
     CDS             12951..14237
                     /locus_tag="JNC24_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HAY9158632.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            14457..15953
                     /locus_tag="JNC24_001882"
     CDS             14457..15953
                     /locus_tag="JNC24_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAY9158633.1"
                     /translation="MQGTKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNIGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASNSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAAATSVATDDVHPEVTDIPPAQ"
     gene            complement(16049..17197)
                     /locus_tag="JNC24_001883"
     CDS             complement(16049..17197)
                     /locus_tag="JNC24_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HAY9158634.1"
                     /translation="MFKSDAARTRQRLSARKGRTLPPGRGKFPHSTTESFRNTFWLKK
                     IMEHCFNMVDQQTTTAQTNANFLQIRFTTMSKKIAVIGECMIELSEKGADVKRGFGGD
                     TLNTSVYIARQVDPAALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGL
                     YYIETDSTGERTFYYWRNEAAAKFWLESEQSAAICEELANFDYLYLSGISLAILSPTS
                     REKLLSLLRECRANGGKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDA
                     LWGQQPVEDVIARTHNAGVKEVVVKRGADSCLVSIAGEGLVDVPAVKLPKEKVIDTTA
                     AGDSFSAGYLAVCLTGGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            17210..17977
                     /gene="pdeH"
                     /locus_tag="JNC24_001884"
     CDS             17210..17977
                     /gene="pdeH"
                     /locus_tag="JNC24_001884"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HAY9158635.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFVEHDLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPMETLNTAVLAL"
     gene            18047..20107
                     /locus_tag="JNC24_001885"
     CDS             18047..20107
                     /locus_tag="JNC24_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HAY9158636.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWFKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYVFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIATNIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLNIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPTAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(20289..21611)
                     /locus_tag="JNC24_001886"
     CDS             complement(20289..21611)
                     /locus_tag="JNC24_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HAY9158637.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVASI
                     LGASVAPYIAAWLQTNYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(22022..23035)
                     /gene="yhjD"
                     /locus_tag="JNC24_001887"
     CDS             complement(22022..23035)
                     /gene="yhjD"
                     /locus_tag="JNC24_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HAY9158638.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            complement(23084..24055)
                     /locus_tag="JNC24_001888"
     CDS             complement(23084..24055)
                     /locus_tag="JNC24_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY9158639.1"
                     /translation="MLEKTINNAICALLFRCEQQSVKEMDKIHAMQLFIKVAELESFS
                     RAADFFALPKGSVSRQIQALEHQLGTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSE
                     LDGLFQQDATSISGKLRIDIPSGIAKNLLLPRLSEFLYQHPGIELELSSHNRPVDILH
                     DGFDCVIRTGALPEDGVIARPLGKLTMVNCASPHYLTRFGYPQSPDDLTSHAMVRYTP
                     HLGVHPLGFEVASVNGVQWFKSGGMLTVNSSENYLAAGLAGLGIIQIPRIAVREALRA
                     GQLIEVLPGYRAEPLSLSLVYPQRRELSRRVNLFMQWLAGVMKEYLD"
     gene            24503..>25104
                     /locus_tag="JNC24_001889"
     CDS             24503..>25104
                     /locus_tag="JNC24_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAY9158640.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQQRIPGVSIQGASQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
BASE COUNT         5894 a         6365 c         6991 g         5854 t
ORIGIN      
        1 ttagcttgtg aatcaattta gaaggtcaca agacgtgtga ttttagtctt ttcgttttta
       61 actttttctg ttgcgtaacg gaaagtcaaa aagtgagcaa attcccgtct cgccgtaaac
      121 aataaaccgg cgaaatgctc acggttagaa ttgtttcatg aacggtacgg ttatttcata
      181 gggatcaagc aaaatgaata acaatgaacc agatactctg cctgatcccg cgataggcta
      241 tatcttccag aatgatattg tggcgttaaa gcaggcattt tcactgcctg atattgatta
      301 tgccgatatt tcccaacgcg aacagttggc cgcggcatta aaacgctggc cgttgctggc
      361 agagtttgcg caacaaaagt aggggattgg tgaatggccg tactgggatt gcagggggtg
      421 cggggaggcg tggggacaac aaccatcacc gccgcattag cctggtcatt acaaatgttg
      481 ggagaaaatg tcctggtggt cgatgcctgc ccggacaact tgttgcgcct gtcatttaat
      541 gttgatttta cccaccgtca gggctgggcc agagcgatgc tggatggcca ggactggcgt
      601 gacgctgggt tgcgctacac ctcgcagctc gatttgctgc cttttggtca gttatccatt
      661 gaagaacaag aaaatccaca gcactggcaa acccggctga gcgatatttg ctccggctta
      721 cagcaactaa aagccagcgg gcgttaccag tggattttaa tcgacttacc gcgtgatgcc
      781 tcgcagataa cccaccagct gctgagtttg tgcgatcact cgctggcaat cgtcaatgtg
      841 gatgccaact gccatatccg actgcatcag caagcgctgc cggatggcgc acatattttg
      901 attaatgact tccgtattgg cagtcaggtt caggacgata tttaccagct ttggttgcaa
      961 agccagcgcc gattactgcc gatgctcatt catcgtgatg aagcgatggc tgaatgcctg
     1021 gcggctaagc aaccagtagg tgaatatcgc agtgatgcgc tggcggctga agagatactg
     1081 acgctggcga actggtgcct gttgaactac tccgggctga aaacgccagt cgggagtgca
     1141 tcatgagtat cctgacccgg tggttgctta tcccgccggt caacgcgcgg cttatcgggc
     1201 gttatcgcga ttatcgtcgt cacggtgcgt cggctttcag cgcgacgctc ggctgtttct
     1261 ggatgatcct ggcctggatt tttattccgc tggagcaccc gcgctagcag cgtattcgcg
     1321 cagaacataa aaacctgtat ccgcatatca acgcctcgcg tccgcgtccg ctggacccgg
     1381 tccgttatct cattcaaaca tgctggttac tgatcggtac atcgcgcaaa gaaacgccga
     1441 aaccgcgcag gcgggcattt tcaggtctgc agaatattcg tggacgttac catcaataga
     1501 tgaacgagct gcctgagcgc gttagccata aaacacagca tcttgatgag aaaaaagagc
     1561 tcggtcattt gagtgccggg gcgcggcggt tgatcctcgg tatcatcgtc accttctcgc
     1621 tgattctggc gttaatctgc gttactcaac cgtttaaccc gctggcgcag tttatcttcc
     1681 tgatgctgct gtggggtgta gcgctgatcg tacggcggat gccggggcgc ttctcggcgc
     1741 taatgttgat tgtgctgtcg ctgaccgttt cttgccgtta tatctggtgg cgttacacct
     1801 ctacgctgaa ctgggacgat ccggtcagcc tggtgtgcgg gcttattctg ctcttcgctg
     1861 aaacgtacgc gtggattgtg ctggtgctcg gctacttcca ggtagtatgg ccgctgaatc
     1921 gtcagccggt gccattgccg aaggatatgt cgctgtggcc gtcagtggat atctttgtcc
     1981 cgacttacaa cgaaaatctc aatgtggtga aaaataccat ttacgcctcg ctgggtatcg
     2041 actggccgaa agacaaactg aacatctgga tcctcgatga cggcggcagg gaagagtttc
     2101 gccagtttgc gcaaaacgtg ggggtgaaat atatcgcccg caccactcat gaacatgcga
     2161 aagcgggcaa catcaacaat gcgctgaaat atgccaaagg cgagttcgtg tcgattttcg
     2221 actgcgacca cgtaccaacg cgatcgttcc tgcaaatgac catgggctgg ttcctgaaag
     2281 agaaacagct ggcgatgatg cagactccac accacttctt ctcgccggac ccgtttgaac
     2341 gcaacctggg acgttttcgt aaaacgccga acgaaggcac gctgttctat ggtctggtgc
     2401 aggatggcaa cgatatgtgg gacgccactt tcttctgcgg ttcctatgcg gtgattcgtc
     2461 gtaagccgct ggatgaaatt ggcggcattg ctgtcgaaac cgtgactgaa gatgcgcata
     2521 cttctctgcg tttgcaccgt cgtggctata cctccgcgta tatgcgtatt ccgcaggcgg
     2581 cggggctggc gaccgaaagt ctgtcggcgc atatcggtca gcgtattcgc tgggcgcgcg
     2641 ggatggtaca aatcttccgt ctcgataacc cgctcaccgg taaaggactg aagtttgctc
     2701 agcggctgtg ctacgtcaac gccatgttcc acttcttgtc gggcattcca cggctgatct
     2761 tcctgactgc gccgctggcg ttcctgctgc ttcatgccta catcatctat gcgccagcgt
     2821 tgatgatcgc cctattcgtg ctgccgcata tgatccatgc cagcctgacc aactcgaaga
     2881 tccagggcaa atatcgccac tctttttgga gtgaaatcta cgaaacggtg ctggcgtggt
     2941 atatcgcacc accgacgctg gtggcgctga ttaacccgca caaaggcaaa tttaacgtca
     3001 ccgccaaagg tggactggtg gaagaagagt acgtcgactg ggtgatctcg cggccctaca
     3061 tcttccttgt tctactcaac ctggtgggcg ttgcggtagg catctggcgc tacttctatg
     3121 gcccgccaac cgagatgctc accgtggtcg tcagtatggt gtgggtgttc tacaacctga
     3181 ttgttcttgg cggcgcagtt gcggtatcgg tagaaagcaa acaggtacgc cgatcgcacc
     3241 gcgtggagat gacgatgccc gcggcaattg cccgcgaaga tggtcacctc ttctcgtgta
     3301 ccgttcagga tttctccgac ggtggtttgg ggatcaagat caacggtcag gcgcagattc
     3361 tggaagggca gaaagtgaat ctgttgctta aacgcggtca gcaggaatac gtcttcccga
     3421 cccaggtggc gcgcgtgatg ggtaatgaag ttgggctgaa attaatgccg ctcaccaccc
     3481 agcaacatat cgattttgtg cagtgtacgt ttgcccgtgc ggatacatgg gcgctctggc
     3541 aggacagcta cccggaagat aagccgctgg aaagtctgct ggatattctg aagctcggct
     3601 tccgtggcta ccgccatctg gcggagtttg cgccttcttc ggtgaagggc atattccgtg
     3661 tgctgacttc tctggtttcc tgggttgtat cgtttattcc gcgccacccg gagcggagcg
     3721 aaacggcaca accatcggat caggctttgg ctcaacaatg atgataacgc gatgaaaaga
     3781 aaactattct ggatttgtgc agtggctatg aggatgagtg cgttcccctc tttcatgacg
     3841 caggcgacgc cagcaacgca accactgatc aatgctgagc cagctgtagc cgcccagacg
     3901 gaacaaaatc cgcaggtggg gcaagtgatg ccgggcgtgc agggcgctga tgcgccagtc
     3961 gtggcgcaga acggtccttc gcgtgatgtg aagctgacct ttgcgcaaat tgcgccgccg
     4021 ccgggcagca tggtgctacg tggcattaac ccgaacggca gcattgagtt tggtatgcgc
     4081 agcgatgaag tggtgacgaa ggcgatgctc aacctcgaat acacgccatc gccatcgtta
     4141 ctgcctgtcc agtcgcagtt aaaggtttat ctcaatgatg agctgatggg cgtgctgcca
     4201 gtgaccaaag aacagttggg taaaaaaacg ctggcgcaaa tgcccattaa cccactgttt
     4261 attaccgact tcaaccgtgt gcggctggag tttgtcggcc attatcagga cgtgtgcgaa
     4321 aacccggcca gcaccacgct ttggctggat gttgggcgga gcagtggact ggatctgacc
     4381 tatcagaccc tgaatgtgaa gagtgacctg tcacacttcc cggtgccatt ctttgacccg
     4441 cgcgataacc gtactaacac cttgccgatg gtctttgcgg gtgcgccgga tgttgggctg
     4501 caacaagcat ctgccattgt cgcctcgtgg tttggttcgc gttctggctg gcgtgggcag
     4561 aacttcccgg tgctctataa ccaattgccg gatcgcaatg ccattgtctt tgcaaccaac
     4621 gacaaacggc cggacttcct gcgcgatcat ccggcggtaa aagccccggt gattgagatg
     4681 attaaccatc cgcagaatcc ttacgtcaaa ctgctggtgg tgtttggtcg tgacgacaaa
     4741 gacctgttgc aggcagcgaa aggtatcgct cagggtaaca ttctgttccg tggtgaaagc
     4801 gtggtagtga atgaagtgaa accgctgcta ccgcgtaagc cgtacgatgc gccgaactgg
     4861 gtacgtaccg atcgtccggt cacatttggc gaactgaaaa cctatgaaga acagttacaa
     4921 tccagcggtc ttgagccagc agcgattaac gtttcgctaa acctgccgcc ggatctctac
     4981 ctgatgcgca gtaccggcat tgatatggat attaattacc gctacaccat gccgccggtg
     5041 aaagacagtt cgcggatgga tatcagcctg aataaccagt tcctgcaatc cttcaacctg
     5101 agcagtaaac aggaggcgaa ccgcctgctg ctgcggattc cggtattaca aggtttgctg
     5161 gatggcaaaa cggatgtctc tattccggcg ctgaaactgg gcgcgaccaa ccagctgcgc
     5221 ttcgactttg agtatatgaa cccgatgccg ggcggttcgg tggataactg tattaccttc
     5281 cagccggtgc agaatcatgt ggtgattggt gacgattcca ccatcgactt ctcgaagtat
     5341 taccacttca tcccgatgcc ggatctacgc gcctttgcta acgcgggctt cccgttcagc
     5401 cggatggcgg atctgtcgca aaccatcacc gtgatgccga aaacgcctaa cgaagcacag
     5461 atggaaacgt tgctgaatac tgttggtttt atcggcgcac agacgggctt cccggcgatt
     5521 aatctgacgg tgaccgatga tggcagcacc attcagggca aagatgccga catcatgatc
     5581 atcggtggta tcccggacaa attaaaagac gataagcaga tcgacctgtt ggtgcaggcg
     5641 accgaaagct gggtgaaaac accgatgcgc cagaccccgt tccccggcat tgtgccggac
     5701 gagagcgatc gtgcggcaga aacccagtca acgctgacct cttccggtgc gatggcggcg
     5761 gtgattggct tccagtcgcc gtataacgac cagcgcagcg tgattgcgct gctggcagat
     5821 agcccacgcg gttatgaaat gcttaacgat gcggtgaacg atagcggcaa acgcgccacc
     5881 atgttcggtt cggtcgcggt gatccgcgag tccggtatca acagcctacg tgttggcgac
     5941 gtttattacg taggtcatct gccgtggttc gagcgcttgt ggtatgcgct ggcaaaccat
     6001 ccgattctgc tggcggtgct ggcggcaatc agtgtgatat tgctggcatg ggtactgtgg
     6061 cgtctgctgc gaattattag tcgtcgtcgt cttaacccgg ataacgagta attgaagatg
     6121 aatgtgttgc gtagtggaat cgtgacgatg ctgctgttgg ctgcctttag tgttcaggca
     6181 gcctgtacct ggcctgcctg ggagcagttt aaaaaggatt acatcagtca ggaagggcgc
     6241 gtcatcgccc ccagcgacgc gcgcaaaatc accacctccg aagggcaaag ttacggtatg
     6301 ttctttgccc tggcggctaa cgaccgtgta gctttcgata atattctcga ctggacgcag
     6361 aacaatctcg ctcagggctc tttaaaagaa cgtttgcccg cctggctgtg gggcaagaaa
     6421 gagaacagta agtgggaagt gctggacagc aattcggcct ccgatggtga tgtctggatg
     6481 gcctggtcgt tgctggaggc ggggcgtttg tggaaagagc agcgttatac cgacatcggc
     6541 agcgcgttgc taaaacgtat cgcgcgggag gaagtggtga cggtgcctgg gctgggttcc
     6601 atgttgttac cgggcaaagt gggttttgct gaggataaca gctggcgttt taaccccagc
     6661 tacctgccgc cgacgctggc gcagtatttc acccgctttg gcgcgccgtg gaccacgctg
     6721 cgcgaaacca atcaacgttt attgctggaa accgccccga aaggtttttc gccagactgg
     6781 gtgcgctatg agaaagacaa aggctggcag ctaaaagccg aaaaaacatt gatcagcagc
     6841 tacgacgcta tccgcgttta catgtgggta ggcatgatgc ctgacagcga tccgcaaaaa
     6901 gcgcggatgc tcaaccggtt taaaccgatg gcgacattca ctgagaaaaa cggttatccg
     6961 ccggaaaaag tggatgtggc tacggggaaa gcgcagggta aaggaccgat cggtttttct
     7021 gccgccatgc tgcccttttt acaaaaccgc gatgcgcagg ccgttcagcg ccagcgcgtg
     7081 gccgataact ttcccggcag cgatgcctat tacaactatg tgctgaccct gtttggacaa
     7141 ggctgggatc aacaccgttt ccgcttctcg acaaaaggtg agttattacc tgactggggc
     7201 caggaatgcg caaattcaca ctaaacatat tcacgctttc cctcggtctg gccgtcatgc
     7261 cgatggtcga ggcagcacca accgctcagc aacagttgct ggagcaagtt cggttaggcg
     7321 aagcgaccca tcgtgaagat ctggtgcaac agtcgttata tcggctggaa cttattgatc
     7381 cgaataaccc ggacgtcgtt gccgcccgtt tccgttcttt gttacgtcag ggcgatattg
     7441 atggcgcgca aaaacagctc gatcggctgt cgcagttagc gccgagttca aatgcgtata
     7501 aatcgtcgcg gactacgatg ctactttcca cgccggatgg tcgtcaggca ctgcaacagg
     7561 cacgattgca ggcgacgacc ggtcatgcag aagaagctgt ggcgagttac aacaaactat
     7621 tcaacggtgc gccgccggaa ggtgacattg ctgtcgagta ctggagtacg gtggcgaaaa
     7681 ttccggctcg ccgtggcgaa gcgattaatc agttaaaacg catcaatgcg gatgcaccgg
     7741 gcaatacggg cctgcaaaac aatctggcgc tattgctgtt tagtagcgat cgccgtgacg
     7801 aaggttttgc cgtcctggaa cagatggcaa aatcgaacgc cgggcgcgaa ggggcctcta
     7861 aaatctggta cgggcagatt aaagacatgc ccgtcagtga tgccagtgtg tcggcgctga
     7921 aaaaatatct ctcgatcttt agtgatggcg atagcgtggc ggctgcgcaa tcgcaactgg
     7981 cagaacagca aaaacagctg gccgatcctg ctttccgcgc tcgtgcgcaa ggtttagcgg
     8041 cggtggactc tggtatggcg ggtaaagcca ttcccgaact acaacaggcg gtgcgggcga
     8101 acccgaaaga cagtgaagct ctgggggcgc tgggccaggc gtattctcag aaaggcgatc
     8161 gcgccaatgc agtggcgaat ctggaaaaag ccctcgcact ggacccgcac agcagcaaca
     8221 acgacaaatg gaacagtctg ctgaaagtaa accgctactg gctggcgatc cagcagggcg
     8281 atgctgcgct gaaagccaat aatcctgacc gggcagaacg cctgttccag caggcgcgta
     8341 atgtcgataa caccgacagt tatgcagtgc tggggctggg cgatgtggcg atggcgcgaa
     8401 aagattatcc cgccgccgaa cgttattatc agcagacctt gcgtatggac agcggcaaca
     8461 ctaacgccgt gcgcgggctg gcaaatattt accgccagca atcgccagaa aaagctgaag
     8521 cgtttatcgc ctcgctctct gccagtcagc ggcgtagcat tgatgatatc gaacgcagcc
     8581 tgcaaaacga ccgtctggca cagcaggcag aggcactgga aaaccagggc aaatgggcgc
     8641 aggcggcagc acttcagcgg caacgactgg cgctggaccc cggcagcgta tggattactt
     8701 accgactttc gcaggatctc tggcaggccg gacaacgcag ccaggccgat acgttaatgc
     8761 gcaatctggc gcagcagaag tcgaacgacc cagagcaggt ttacgcttac gggctgtacc
     8821 tacgcttacg ggctgtacct ctctggtcat gaccaggaca gagcggcgct ggcgcatatc
     8881 aacagcctgc cgcgtgcgca gtggaacagc aatattcagg agctggttaa tcgactgcaa
     8941 agcgatcagg tgctggaaac cgctaaccgc ctgcgagaaa gcggcaaaga ggcagaagcg
     9001 gaagcgatgc tgcgccagca accaccttcc acgcgtattg acctcacgct ggctgactgg
     9061 gcgcaacaac gacgtgatta caccgccgcc cgcgctgcat atcagaatgt cctgacgcgg
     9121 gagccagcta acgccgacgc cattcttggt ctgacggaag tggatattgc tgccggtgac
     9181 aaagcggcgg cacgtagcca gctggcgaaa ctgcccgcta ccgataacgc ctcgctgaac
     9241 acacagcggc gcgtggcgct ggcacaggcg cagcttggcg ataccgcagc agcgcagcgg
     9301 acgtttaata agttgatccc gcaggcaaaa tctcagccac cgtcgatgga aagcgcgatg
     9361 gtgctgcgtg atggtgcgaa gtttgaagcg caggcgggcg atccaacgca ggcgctggaa
     9421 acctacaaag acgccatggt cgcatccggt gtgactacga cgcgtccgca ggataacgac
     9481 acctttaccc gactgacccg taacgacgag aaagatgact ggctgaaacg tggcgtgcgc
     9541 agcgatgcgg cggacctcta tcgccagcag gatcttaacg tcacccttga gcacgattac
     9601 tggggttcga gcggcaccgg tggttactcc gatctgaaag cgcacactac catgttgcag
     9661 gtggatgcgc cgtattctga cgggcggatg ttctttcgca gtgatttcgt caatatgaac
     9721 gtcggcagtt tctccactaa tgccgatggc aaatgggatg acaactgggg cacctgtaca
     9781 ttacaggact gtagcggcaa ccgcagccag tcggattccg gtgccagcgt ggcggtcggc
     9841 tggcgaaatg acgtctggag ctgggatatc ggtaccacgc cgatgggctt caacgtggtg
     9901 gatgtggtcg gcggcatcag ttacagcgat gatatcgggc cgctgggtta caccgttaac
     9961 gcccaccgtc ggcccatctc cagttctttg ctggcctttg gtgggcaaaa agactccccg
    10021 agcaataccg ggaaaaaatg gggtggcgta cgtgccgacg gtgtggggct aagtctgagc
    10081 tacgataaag gtgaagcaaa cggcgtctgg gcatcgctta gtggcgacca gttaaccggt
    10141 aaaaatgtcg aagataactg gcgcgtgcgc tggatgacgg gctattacta taaggtcatt
    10201 aaccagaaca atcgccgcgt cacaatcggc ctgaacaaca tgatctggca ttacgacaaa
    10261 gatctgagtg gctactcact cggtcagggc ggttactaca gtccgcagga atacctgtcg
    10321 tttgccatac cggtgatgtg gcgggagcgc acggaaaact ggtcgtggga gctgggggcg
    10381 tctggctcgt ggtcgcattc acgcaccaaa accatgccgc gttatccgct gatgaatctg
    10441 atcccgaccg actggcagga agaagctgcg cggcaatcca acgatggcgg cagcagtcag
    10501 ggcttcggct acacggcgcg ggcattactt gaacgacgtg ttacttccaa ctggtttgtt
    10561 ggcacggcaa ttgatatcca gcaggcgaaa gattacgcac ccagccattt cctgctctac
    10621 gtacgttatt ccgccgccgg atggcagggt gacatggatt taccgccgca gccgctgata
    10681 ccttacgccg actggtaagt tttcagatag cgcctctctt aatgccgctg cgatcgggta
    10741 tactcgggcg gcaatctggg atttccgggg ggagacaatt tgcgcgtaag tcgctcgtta
    10801 acaatcaagc agatggcaat ggtggcagcc gttgtcctgg tgttcgtttt tattttttgc
    10861 accgttttgc tgttccatct ggtccagcag aatcgctata acacggctac gcaactggaa
    10921 agcattgctc gctctgtccg cgaaccctta tcttcagcta ttttgaaagg cgatattccc
    10981 gaagcggaag ctattcttgc cagcattaaa ccggcaggcg tggtcagccg tgccgatgta
    11041 gtgctgccta accagttcca ggcgctgcgt aaaagtttta ttccagagcg cccggtgccg
    11101 ataatggtta ctcgcctgtt tgagctaccg gttcaaatct cgctgggcgt ttactcgctc
    11161 gaacgtccgg caaacccgca gccaattgcc tatctggtac tacaggcgga ttccttccgt
    11221 atgtataagt tcgtgatgag caccctctca acgttagtga ccatttactt acttttgtcg
    11281 cttatcctga ccgtcgccat cagctggtgc attaaccgcc tgattttgca tccgttacgc
    11341 aatattgctc gcgaacttaa cgccatccca gcccaggagc ttgttggtca ccaactggca
    11401 ttaccgcgtc tgcatcagga cgatgaaatc ggtatgttgg tgcgcagtta caacctcaac
    11461 cagcaattgc tgcagcgcca ttatgaagaa cagaacgaaa atgcgatgcg cttcccggtg
    11521 tcggatttgc cgaacaaagc cttgctgatg gagatgctgg agcaggttgt cgcgcgtaaa
    11581 caaaccaccg cgctgatgat catcacctgt gaaaccctgc gtgatactgc gggcgtgctg
    11641 aaagaggcgc aacgagaaat tctgctgctg acgctggtgg aaaaactcaa atcggtactg
    11701 tcgccacgta tgatcctcgc gcagattagc ggttatgact ttgctgtcat tgccaacggt
    11761 gtacaggaac cgtggcacgc aatcacctta ggtcagcaag tgctcaccat catgagcgag
    11821 cgcctgccga ttgaacgtat tcaactccgt ccgcactgta gcattggcgt ggcgatgttc
    11881 tacggcgatc tcaccgccga acagctttac agtcgcgcta tttctgcggc atttaccgct
    11941 cgccataaag gcaagaatca gattcagttc tttgatccgc agcagatgga agccgcccag
    12001 aagcggttga cggaagagag cgatatcctt aatgcactgg aaaatcatca gtttgctatt
    12061 tggttacagc cacaggtcga gatgaccagc ggtaaactgg tcagtgcgga agtgttactg
    12121 cgtatccagc aaccggatgg cagttgggac ctgccggatg gcttaatcga tcgcattgag
    12181 tgctgtgggc tgatggttac cgtcggtcac tgggtgctgg aagagtcctg tcgattgctt
    12241 gcagcctggc aagagcgcgg cattatgctg cccttgtcgg taaacctctc tgcgctgcaa
    12301 ctgatgcacc caaatatggt ggcggatatg ctggaactgt taacccgcta tcgcattcag
    12361 ccgggaacac tgattctgga agtgacagaa agccgacgta ttgacgaccc tcatgctgcg
    12421 gtggcaatcc tccgtccgct gcgcaatgcc ggagttcggg tggcgctgga tgatttcggc
    12481 atgggctacg cagggctgcg tcagctgcag catatgaaat cgttgccaat cgacgtactg
    12541 aaaatcgaca aaatgtttgt tgaaggcttg ccggaagata gcagcatgat tgctgcaatt
    12601 atcatgctgg cgcagagcct gaacttacaa atgattgccg aaggcgtgga gactgaagca
    12661 caacgcgact ggctggcaaa agcgggcgtt ggtattgccc agggcttcct ttttgctcac
    12721 ccactcccta ttgaaatctt cgaagagagt tacctggaag aaaagtagct accccaaact
    12781 gattacaaaa ctttaaaaag tgctggtttg tgcgagccag ctcaaacttt ttaacctttt
    12841 tgtttcaatt atgatccagg tacatttctg tgatgttgtc tgggtgttat tttaaggccg
    12901 caggtacccc ataaccttac aagacctgtg gttttactaa aggacaccct atgaaaacct
    12961 ctctgtttaa aagcctttac tttcaggtcc tgacagcgat agccattggt attctccttg
    13021 gccatttcta tcctgaaata ggcgagcaaa tgaaaccgct tggcgacggc ttcgttaagc
    13081 tcattaagat gatcatcgct cctgtcatct tttgtaccgt cgtaacgggc attgcgggca
    13141 tggaaagcat gaaggcggtc ggtcgtaccg gcgcagtcgc actgctttac tttgaaattg
    13201 tcagtaccat cgcgctgatt attggtctta tcatcgttaa cgtcgtgcag cctggtgccg
    13261 gaatgaacgt cgatccggca acgcttgatg cgaaagcggt agcggtttac gccgatcagg
    13321 cgaaagacca gggcattgtc gccttcatta tggatgtcat cccggcgagc gtcattggcg
    13381 catttgccag cggtaacatt ctgcaggtgc tgctgtttgc cgtactgttt ggttttgcgc
    13441 tccaccgtct gggcagcaaa ggccaactga tttttaacgt catcgaaagt ttctcgcagg
    13501 tcatcttcgg catcatcaat atgatcatgc gtctggcacc tattggtgcg ttcggggcaa
    13561 tggcgtttac catcggtaaa tacggcgtcg gcacactggt gcaactgggg cagctgatta
    13621 tctgtttcta tattacctgt atcctgtttg tggtgctggt attgggttca atcgctaaag
    13681 cgactggttt cagtatcttc aaatttatcc gctacatccg tgaagaactg ctgattgtac
    13741 tggggacttc atcttccgag tcggcgctgc cgcgtatgct cgacaagatg gagaaactcg
    13801 gctgccgcaa atcggtggtg gggctggtca tcccgacagg ctactcgttt aaccttgatg
    13861 gcacatcgat atacctgaca atggcggcgg tgtttatcgc ccaggccact aacagtcaga
    13921 tggatatcgt ccaccaaatc acgctgttaa tcgtgttgct gctttcttct aaaggggcgg
    13981 caggggtaac gggtagtggc tttatcgtgc tggcggcgac gctctctgcg gtgggccatt
    14041 tgccggtagc gggtctggcg ctgatcctcg gtatcgaccg ctttatgtca gaagctcgtg
    14101 cgctgactaa cctggtcggt aacggcgtag cgaccattgt cgttgctaag tgggtgaaag
    14161 aactggacca caaaaaactg gacgatgtgc tgaataatcg tgcgccggat ggcaaaacgc
    14221 acgaattatc ctcttaatct caccacttat gcccgcactc ccttccccga gtgcgggtgt
    14281 ttgcgcataa ttgacccctg tgcctgaact taccacctgg gtgagttcag gtttatttca
    14341 cttcttccgt gacattttca tgttcttgcg gtctaacacg aagtgttttt aacgtcattt
    14401 agactgactg acaacgggca gttttttatt accaggatag ttgatcaggg gttcacatgc
    14461 agggcacaaa aattcgactt ttagcgggcg gtttgctgat gatggccact gctggctatg
    14521 tgcaggcaga tgcgctccag cctgatccag catggcaaca ggggacgctt tccaacggtt
    14581 tacagtggca agtgctgacc accccccagc gtcccagcga tcgtgttgaa attcgcctgc
    14641 tggttaatat cggttcgctc gccgaaagta cacaacagag cggttacagt cacgccatcc
    14701 ctcgtattgc gctaacgcaa agcggtggcc ttgacgcagc acaggcgcgt tcattgtggc
    14761 agcaggggat cgaccctaaa cgcccgatgc cgccggtaat tgtctcttat gacaccacgc
    14821 tgtttaatct gagtttgccc aataaccgta acgatttgct gaaagaagcg ctctcttatc
    14881 tggcaaatgc cactggcaaa ttgaccatca caccagaaac catcaaccac gcgctgcaaa
    14941 gtcaggacat ggtggcaacc tggcctgccg atactaaaga gggctggtgg cgctatcgtc
    15001 tgaaagggtc aaccttgtta ggtcacgatc ctgccgatcc gctgaaacaa cccgttgaag
    15061 cggaaaaaat taaagatttc tatcagaaat ggtacacccc ggatgcaatg acgctactgg
    15121 tggtgggaaa cgtggatgcg cgctcggttg tcgaccaaat caacaaaacg tttggcgaac
    15181 tgaaaggcaa acgtgaaacg ccagctccgg tgccgacgct ttctccgctg cgtgcggaag
    15241 cggtgagtat tatgactgac gcggtgcgtc aggaccggtt atctatcatg tgggatacgc
    15301 cgtggcagcc gattcgtgaa tcagccgcac tgctgcgcta ctggcgtgcg gacctggccc
    15361 gcgaggcgct gttctggcat gttcagcaag cgttaagcgc cagtaacagc aaagacatcg
    15421 gtcttggatt tgactgccgt gtgctgtatc tgcgtgcgca gtgtgccatc aacatcgaat
    15481 caccaaacga caagctgaac agcaacctta atctggtggc gcgtgaactg gcgaaggttc
    15541 gcgataaagg tctgccggaa gaagagttca atgcgttagt ggcgcaaaag aaactggagc
    15601 tgcagaaact gtttgccgcc tatgcgcggg ctgataccga tattctgatg ggtcagcgga
    15661 tgcgttcgct gcaaaatcag gtcgtcgata tcgcgccgga gcagtatcag aaactgcggc
    15721 aggatttcct taacagcctg acggtggaga tgttaaatca ggatctgcgt cagcagttgt
    15781 cgaatgatat ggcgttaata ctgctgcagc cgaaaggcga gccggaattt aacatgaaag
    15841 cgttgcaggc ggcctgggat caaatcatgg ccccatcgac tgctgctgcc gccacctctg
    15901 tcgccacgga tgacgtacat cctgaagtga cggatattcc acctgcacag taatgcctga
    15961 tggtgatgag ttagcacaac catcaggtat ttatggatga gctggataga ggggtaacac
    16021 tttatccagc cttttgcata tgctgcgttt acgctggcat cgcctcacgc gggataatcg
    16081 cgccgcgata ctgaataacg gtacttgcgg tcaggtgccc gcgtttcgcc gcgttttccg
    16141 cactgccgcc cgtcaaacat accgccagat aaccggcgct gaaagagtcg ccagctgcgg
    16201 tggtatcgat cactttttct ttcggcagtt tcaccgccgg aacatccact aacccttcgc
    16261 cagcaatgga caccaggcaa gaatccgccc cgcgtttcac caccacttct ttcacgcccg
    16321 cgttatgggt gcgcgcaatg acgtcttcca ccggctgttg accccacagc gcgtcttcat
    16381 cgtccagcgt caggaaggcg atatccgtgc attccagcat ttgttggtac acctgctgtg
    16441 tctcttcttt gctggcccac aggcgcggac gatagttatt gtcgaaaatc acttttccgc
    16501 cgttggcgcg gcattcgcgc agcagggaaa gcagcttttc gcggctggtc gggcttaaga
    16561 tcgccaggct aatcccgctc aggtagagat aatcgaaatt cgccagctct tcgcaaatcg
    16621 ccgcagactg ctcactctcc agccagaatt tggcggcggc ttcgttccgc cagtagtaga
    16681 acgtacgctc gccggtgctg tcggtttcaa tgtagtaaag gcccggcaga cggttttcca
    16741 tccgttgggt cagggaagta tcaacgttct cgccgtgcca ggcgtccagc atttgctggc
    16801 taaaactgtc cgttcccagc gccgttacgt aatgaacggt taatgccgca ggatcgacct
    16861 gacgggcgat atagacggaa gtgttcaggg tatcgccgcc gaaaccgcgc ttaacgtccg
    16921 cgcctttctc ggaaagctca atcatgcatt cgccaatcac ggcaatcttt ttggacatag
    16981 tcgtgaacct gatctgtaaa aaattagcgt tagtttgcgc tgtggtggtt tgctggtcaa
    17041 ccatattaaa acagtgttcc attatttttt tgagccagaa cgtattcctg aaagattccg
    17101 ttgtggagtg aggaaattta ccccggccag gcggtaaagt tctgcccttg cgcgccgata
    17161 atctttgtcg agtccgggca gcatcacttt taaacacagg acatctttga tgataaggca
    17221 ggttatccag cgaataagca accctgaagc aagcatcgag agcttgcagg aacggcgttt
    17281 ttggttgcag tgtgagcgtg cttacacctg gcagccgatc tatcaaacat gcggacggtt
    17341 aatggccgtg gagctattaa cggtggtcac gcatcccttg aacccttcgc aacgcctgcc
    17401 gccggatcgc tattttactg aaatcaccgt cagccatcgg atggaggttg tgaaagagca
    17461 gattgatttg ctggcgcaaa aagccgactt ctttgtagag cacgacctgc tggcatcggt
    17521 caatattgat ggccctacgc tcatcgccct gcgtcagcaa ccaaaaatcc tgcgccaaat
    17581 tgagcgtctt ccctggctgc gtttcgaact ggtggaacac atccgcctgc cgaaagattc
    17641 aacctttgcc tcgatgtgtg aatttggccc gctgtggctg gatgattttg gcaccgggat
    17701 ggcaaatttc tctgcgctaa gtgaagtgcg ttatgactac atcaaaatcg cgcgagagct
    17761 gttcgtgatg ctgcgtcagt cgccggaagg ccgcacactc ttttctcagc ttttacatct
    17821 aatgaatcgc tattgtcgcg gggtgattgt cgagggcgta gaaacaccgg aagagtggcg
    17881 tgatgttcag aactcgcccg cattcgccgc acaaggctgg tttctttcac gcccggcacc
    17941 gatggaaacg ctgaatacgg cggttctggc gctataagct gcctcatttt ccgctgggct
    18001 ggactatctt taggactggc acaggaagaa tatgaggaag cgaacgatga gcaaggcagg
    18061 caaaataacc gctgcgattt caggggcttt cttgttgttg attgtcgtgg cgatcatttt
    18121 gattgcaact tttgactgga atcgactcaa accgaccatc aaccagaaag tctctgcgga
    18181 gttgaatcgt ccgttcgcta tccgtggcga tctgggcgtg gtgtgggagc ggcaaaaaca
    18241 agaaactggc tggcgcagct gggtgccgtg gcctcatgta catgcagaag acatcattct
    18301 tggtaatcca ccggatattc ccgaagtcac gatggtgcat ttgccgcgag tagaggcaac
    18361 gctggcaccg ctggcgctgc tgaccaaaac ggtctggctg ccgtggttca agctcgaaaa
    18421 gcccgacgcg cgcctgattc gcctctccga aaagaacaat aactggacgt ttaatcttgc
    18481 caacgatgac aacaaagacg cgaatgcaaa gccgtcggca tggtcgtttc ggctggataa
    18541 tattcttttc gatcaagggc ggatcgccat tgatgacaaa gtaagcaaag cggatctgga
    18601 aatttttgtt gatccgttag gcaagccgct gccgttcagc gaagttactg gatcgaaagg
    18661 taaagcggat aaagaaaagg tgggcgatta cgtttttggc ctgaaggcgc agggacgtta
    18721 taacggtgaa ccgctcacgg gtacgggaaa aataggcggt atgctggcgc tgcgtggcga
    18781 agggacgccg tttccggtac aggctgattt ccgttcaggt aatacccgtg ttgcttttga
    18841 tggcgtcgtg aatgacccaa tgaagatggg cggtgtcgat ttacggctta aattttctgg
    18901 cgattcactg ggtgatctct atgaactgac gggcgttctg ctgcccgata ccccgccgtt
    18961 tgaaacggat ggtcggctgg tagcgaaaat cgacactgaa aaatcgtcgg tctttgatta
    19021 tcgcggtttt aatgggcgaa ttggcgatag cgatatccac ggttctctgg tctacaccac
    19081 cggaaagcca cgaccaaaac tggaaggtga tgtcgagtcg cggcaattgc ggctggcgga
    19141 cctgggaccg ttgattggcg ttgattccgg gaaaggggca gaaaagtcga aacggtctga
    19201 acagaagaag ggcgaaaaaa gcgttcagcc tgcgggcaaa gtgctgcctt atgaccgctt
    19261 cgaaaccgat aaatgggacg ttatggatgc cgatgttcgc ttcaaagggc ggcgcattga
    19321 gcatggcagt agcctgccga ttagcgatct ttctactcat atcatcctca aaaatgctga
    19381 cctgcgcctg caaccgctga aatttggcat ggcgggcggc agcattgcga cgaatattca
    19441 tctggaaggc gataaaaagc cgatgcaggg gcgggcagat attcaggctc gtcgactgaa
    19501 actgaaagaa ctgatgcccg atgtggaact gatgcagaag acgctggggg aaatgaacgg
    19561 tgacgcggaa ctacgcggta gcggtaactc ggtggcggcg cttttaggca acagtaacgg
    19621 caacctgaaa ctgttgatga atgacgggct ggtgagccgc aacctgatgg agattgttgg
    19681 gctgaatgtc ggcaactaca ttgtcggtgc gatatttggt gacgatgagg tgcgggtgaa
    19741 ctgcgcggcg gcgaatctga atattgccaa cggcgtggcg cgcccgcaga tttttgcttt
    19801 cgatactgag aacgcgttga ttaatgttac cggcacggca agttttgctt cagaacagct
    19861 ggatttgact attgatccgg agagtaaagg gattcggatt atcacactgc gttcgccgct
    19921 gtatgtgcgt gggacgttta aaaatccgca ggctggggtg aaagccggac cgctgattgc
    19981 ccgtggtgct gttgctgcgg cactggcaac gctggtaaca ccgacggcgg cgttactggc
    20041 actgatctca ccttccgaag gggaggctaa tcagtgtcgg acgatattgt cgcagatgaa
    20101 gaagtgatga taattgccgg atgtctgaca tccggcaatt aaacagattt agatttttgg
    20161 caaagtgtgc tttgttcatg ccggatgcgg cgtgaacgcc ttatccggcc tacaaatcct
    20221 tgtgaattca atatattgta tggaaattca gtcctgataa gcgtagcgca tcaggctttt
    20281 cacacttatt acaacgactg atgtcgcgtc tcatgggtca gcagcagggc gattaacgtc
    20341 aagccagcca tcgccgccag atataacccc accgcaccta acccgtagtt agtctgcaac
    20401 caggctgcga tatacggtgc aacggaagcc ccgagaatcg acgctacgtt gtaagagaat
    20461 gatgctccgg tgtaacgcac ttctgttggg aacagctctg gtaacagcgc gcccatcggc
    20521 ccgaaggtca gccccatcag acttaacccc agcagcagga aggcaaaaac cagaatcggg
    20581 ttgccagaac cgagcagcgg gttaaaggcg aacagcgcga acaggatgat cagcgtggtg
    20641 ataattacca tgcttttacg gcgaccaaag gcatcagcca gtaatccggc gactggcacc
    20701 atcacgccaa aaccaataac tgccatcatc agcatccaca acacttcgtt acgcggcagg
    20761 ccaagcccaa ctggcgcggc ggcggtactg aaggtcatag agtagaccgt catgatgtaa
    20821 aacagcgtat aggttgccag catgatgaac gtacccagta cggttacgcg aacatgtttg
    20881 gtcagcagcg tacccagcgg gatcttcacc tgttttttcg ctttagcgac tttctcaaac
    20941 accggcgact catgcagcga cacgcgaaca tacaggccga taatgaccag taccgccgag
    21001 aagataaatg gcacacgcca gccccagctc ataaactgct cgtcggtcag tagccaggaa
    21061 agcagcaaga aagtgccgtt ggcaaagaag aagccaatcg gtgcgcccag ctgtgggaag
    21121 gagccataca gtgcacgttt gcgcggtggg gcgttttcag tcgccagcag cgccgcgccg
    21181 ccccattcac cgcctaagcc cagaccctga ccaaatcgag ccagcgccag cagcagcggg
    21241 gcgaaaatac caatcgtggc atagcccggc agcagaccaa tcaccacggt tgaaatcccc
    21301 atcgttagca acgaggcgac cagcgtcgct ttacgcccaa cgcgatcgcc aaaatgacca
    21361 aaaacggcag agccaatggg gcgcgcgacg aaggcgatgg cgaaggtggc gagcgactgt
    21421 agcgttgctg ccgttggatc gccctgcggg aagaagatat gcggaaacac aataacggcc
    21481 gcagtggcgt aaatgtaaaa gtcgaagaac tcaatggccg tgccaatgag agaggcgaca
    21541 aggactttat tacgcgagtt gatcggcgtg tattcttgct cgtggtcgag tgttgtggct
    21601 gttgcttgca taatcttttc ttattttttg gctaacgaat agccatatta cgcacagcaa
    21661 aagtgatatt tcaatgtgta acaaatcgcg gaaatggtca aaaaccaggc aaaaagggga
    21721 taatgtttag gccagtgatt tttcttctat gaaatgcttc acataatttt ataaatagtg
    21781 gataaaactg gtttcaggtt aaataaaagt taccaactgg atttatatgc tggggttatg
    21841 agttggaatg aaattgtttg ctcccaaata gatggctgaa gaggctctgg ggaagagttt
    21901 caagaggtta tgtgcgcatt atagccggat gcgatgctgg cgcatcttat ccggcctacg
    21961 gggagccagg ccgaataaaa tcgtatgccg gataaggcgt ttacgccgca tccggcctat
    22021 tttaaggctg cgttttccct ggcattcgcg ggtcgtcttt atattcggcg gtggcaatcc
    22081 acgccgcgca aaacagcgtc aaacgggcga agaagtagaa aaatgccatc agccccagca
    22141 cggaaccaaa tgctgcgcca gagggggatt tcatcagcga tggcagggtg taggtcatca
    22201 cgattttaat cacttcaaaa ccaatagcag ccagaaatgt tccgcgaatc agggcttttt
    22261 tgcgcggacg gtggcgcggt aaccgccaga agatccagaa gaaaagcaga tagttggcga
    22321 agatggaaat cgccaggcca atcaaccgcc acgtcggttt cagccactca atgctgttca
    22381 ggtgcagggc gctaataatc atttgctgcg ccgaaccggc aaccgaggtg atcgaaagcg
    22441 tcacaatcag cgcaatcaac aaaccaatca gcgaaataaa atcacgtaga tattttaccc
    22501 agaacttctc ctgatcttgc ggcgagcgtt cccagacatc gcgcgactgg gcgcgaatcg
    22561 cttcacgcag gttacccatc cagttgatgc cggaataaag cgccacagcc aggccgacaa
    22621 gccctacagt cgtacgctgc tgaacggcgg tgttgatggt gtttttcaac gtggcggcta
    22681 gcgtcggatc gctgatgttt tgcagaattt tgtcgaagat atcctgtagc aacatcggat
    22741 gggaggccag cacaaagccc cctgcggcaa acgacaccat caaaatcggg atcatcgaca
    22801 aaaatgagaa ataggtgata gccgcaccaa actggttacc cagccgatcg ttaaagcgtt
    22861 ctgtcgcgcg aatcaggtgc gcaataaccg gttggcgttg gactttttcg gcagtggtgg
    22921 tgacggtttc cagcgcctgg ctaactttgc cgcctttctc gctattatcc aactgcttaa
    22981 tcggctcgtg ctccagatcc tgggtgggac gtttgatctc gttttcctgc gtcatccgct
    23041 cttttgtcct tctgttgtgg tcattcttaa aaagtatagt cggtcagtcc aggtactctt
    23101 tcattacgcc agccagccac tgcataaaca ggtttacacg ccgggaaagc tcccgacgct
    23161 gcggataaac cagcgaaagg gagagcggct cggcacggta gcctggcaac acttcaataa
    23221 gctgcccggc acgcagggct tcgcgcacgg caatgcgcgg gatctgaata atccccagac
    23281 cggcaagacc ggcggcgaga tagttttcgc tactgtttac cgtcaacatg ccgccagact
    23341 taaaccactg gacgccatta acgctggcaa cctcaaaacc taacggatgt acacccaggt
    23401 gcggtgtata acgcaccatt gcgtgtgaag tcagatcatc ggggctttgc ggataaccaa
    23461 agcgcgtcag atagtgcgga ctggcacagt tgaccatggt cagtttgccg aggggacggg
    23521 cgataacgcc atcttccggt aacgcgccag tgcgtatcac gcaatcaaaa ccatcatgaa
    23581 gaatatctac cggacggtta tggctactca gttccagctc aattcccgga tgctgataga
    23641 gaaattccga cagacgcggc agtaacaggt ttttcgcgat tcctgacggg atgtcgatgc
    23701 gtaatttacc gctgatactg gtggcatcct gttgaaacag accgtccagt tcgctgagat
    23761 tactcaacac atcttttgcc cgttgataat aggtcatgcc ttctggcgtg agtttgaccc
    23821 gtcgcgtagt gcgctggaga agctgggtgc caagttgatg ttccagtgcc tgtatctggc
    23881 gcgaaacact tccctttggc aaagcaaaga aatccgccgc gcgggaaaaa ctttccagct
    23941 ccgcgacttt gatgaacaac tgcattgcgt gaattttatc catttctttg accgattgtt
    24001 gttcacagcg aaacagtaac gcgcatatcg cattgtttat tgttttttct aacatctaat
    24061 gccgggtttc acaatgttgt catgacgaat gtggcgattt ctcagcagga aaagggcgga
    24121 atagctttat gagaaaggtg aatcccctta aacagagcgt atttttcagt gattcccttt
    24181 aaagtgaata gaggacgtat tggctatttg tgcttatttt tgtctcattg ccatttgtta
    24241 ttttggcttg tagtcgcgga atttcatttt taattgcatg cgtactaacc accaatataa
    24301 ggaaatcctc aaaattgttt taaatagtgc cctcgacatg taatttcagg cgtttagctg
    24361 taactattta tatcggtaac tattttctac gcgtcatctc tgtagaatgg ccaccgccaa
    24421 atggcgaatt cttatttttt ctcgcgggta atacccttcc actacttaag tctttttaac
    24481 gtattcaggt ggagattcag ttatgcaaat agtcatgttt gacaggcagt caatatttat
    24541 tcatggaatg aaaatcagtt tacagcagcg tattccagga gtgagtattc agggggccag
    24601 tcaggcagac gagttatggc aaaagctgga aagttaccct gaagccttag ttatgctcga
    24661 tggcgatcag gatggtgagt tttgctactg gttgctgcaa aaaaccgtgg tgcagtttcc
    24721 tgaggttaag gtgttaatca cggcgacgga ttgcaataaa cggtggttac aggaagttat
    24781 tcattttaat gtgctggcca ttgtgcctcg tgattcaacc gtcgagacat ttgcgttggc
    24841 ggtaaatagt gcggcgatgg ggatgatgtt tctgcctgga gactggcgga cgacgccgga
    24901 aaaggacatt aaagatctca aatcgttaag cgcccgccag cgggagattt taaccatgtt
    24961 agcggcgggc gaatcaaata aggagatcgg cagagcgctg aatatcagta ccggaacggt
    25021 aaaagcgcat ctggaatccc tgtatcgccg tctggaagtg aaaaatcgca cccaggcggc
    25081 aatgatgtta aatatctcct cctg
//
LOCUS       DADMKF010000051        24961 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.052, whole genome shotgun sequence.
ACCESSION   DADMKF010000051 DADMKF010000000
VERSION     DADMKF010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24961
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.052"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(166..576)
                     /locus_tag="JNC24_001890"
     CDS             complement(166..576)
                     /locus_tag="JNC24_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 domain-containing protein"
                     /protein_id="HAY9158641.1"
                     /translation="MAQVAIFKQIFDKVRNNLNYHWFYSELKRHNVSHYIYYLATENI
                     HLVLENDNTVLIKGQGKVVNVRFSKNKCLIEATLKGFKSGELSFYEYRKNLATAGVFR
                     WITNIHENKRYYYTFDNSLLFTENIQNTTQIFPH"
     gene            complement(634..867)
                     /locus_tag="JNC24_001891"
     CDS             complement(634..867)
                     /locus_tag="JNC24_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3950 domain-containing protein"
                     /protein_id="HAY9158642.1"
                     /translation="MSTKNRTRRTTTRNIRFPNQMIEQINIALVQKGSGNFSAWVIEA
                     CRRRLCSEKRVSPEANKEKSDITELLRKQVRPD"
     gene            1256..1801
                     /locus_tag="JNC24_001892"
     CDS             1256..1801
                     /locus_tag="JNC24_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HAY9158643.1"
                     /translation="MEVNKKQLADIFGASIRTIQNWQEQGMPVLRGGGKGNEVLYDSA
                     AVIRWYAERDAEIENEKLRREVEELRQASETDLQPGTIEYERHRLTRAQADAQELKNA
                     RDSAEVVETAFCTFVLSRIAGEIASILDGIPLSVQRRFPELENRHVDFLKRDIIKAMN
                     KAAALDELIPGLLSEYIEQSG"
     gene            1776..3701
                     /locus_tag="JNC24_001893"
     CDS             1776..3701
                     /locus_tag="JNC24_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HAY9158644.1"
                     /translation="MNISNSQVNRLRHFVRAGLRSLFRPEPQTAVEWADANYYLPKES
                     AYQEGRWETLPFQRAIMNAMGSDYIREVNVVKSARVGYSKMLLGVYAYFIEHKQRNTL
                     IWLPTDGDAENFMKTHVEPTIRDIPSLLALAPWYGKKHRDNTLTMKRFSNGRGFWCLG
                     GKAAKNYREKSVDVAGYDELAAFDEDIEQEGSPTFLGDKRIEGSVWPKSIRGSTPKVR
                     GTCQIERAASESPHFMRFHVACPHCGEEQYLKFGDKETPFGLKWTPDDPSSVFYLCEH
                     NACVIRQQELDFTDARYICEKTGIWTRDGILWFSSSGEEIEPPDSVTFHIWTAYSPFT
                     TWVQIVKDWMKTKGDTGKRKTFVNTTLGETWEAKIGERPDAEVMAERKEHYSAPVPDR
                     VAYLTAGIDSQRDRYEMRVWGWGPGEESWLIDRQIIMGRHDDEQTLLRVDEAINKTYT
                     RRNGAEMSISRICWDIGGIDPTIVYERSKKHGLFRVIPIKGASVYGKPVASMPRKRNK
                     NGVYLTEIGTDTAKEQIYNRFTLTPEGDEPLPGAVHFPNNPDIFDLTEAQQLAAEEQV
                     EKWVDGRKKILWDSKKRRNEALDCFVYALAALRISISRWQLDLSALLASLQEEDGAAT
                     NKKTLADYARALSGEDE"
     gene            3698..3904
                     /locus_tag="JNC24_001894"
     CDS             3698..3904
                     /locus_tag="JNC24_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lambda prophage-derived head-to-tail joining
                     protein W"
                     /protein_id="HAY9158645.1"
                     /translation="MTRQEELAAARAALHDLMTGKRVATVQKDGRRVEFTATSVSDLK
                     KYIAELEVQTGMTQRRRGPAGFYV"
     gene            3901..5502
                     /locus_tag="JNC24_001895"
     CDS             3901..5502
                     /locus_tag="JNC24_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAY9158646.1"
                     /translation="MKTPTIPTLLGPDGMTSLREYAGYHGGGSGFGGQLRAWNPPGES
                     VDAALLPNFTRGNARADDLVRNNGYAANAIQLHQDHIVGSFFRLSHRPSWRYLGIGEE
                     DARAFSREVEAAWKEFAEDDCCCIDVERKRTFTMMIREGVAMHAFNGELFVQATWDTS
                     SSRLFRTQFRMVSPKRISNPNNTGDSRNCRAGVQINDSGAALGYYVSEDGYPGWMPQK
                     WTWIPRELPGGRASFIHVFEPVEDGQTRGANVFYSVMEQMKMLDTLQNTQLQSAIVKA
                     MYAATIESELDTQSAMDFILGANSKEQRERLTGWIGEIAAYYAAAPVRLGGAKVPHLM
                     PGDSLNLQTAQDTDNGYSVFEQSLLRYIAAGLGVSYEQLSRNYAQMSYSTARASANES
                     WAYFMGRRKFVASRQASQMFLCWLEEAIVRRVVTLPSKARFSFQEARSAWGNCDWIGS
                     GRMAIDGLKEVQEAVMLIEAGLSTYEKECAKRGDDYQEIFAQQVRETMERRAAGLKPP
                     AWAAAAFESGLRQSTEEEKSDSRAA"
     gene            5483..6802
                     /locus_tag="JNC24_001896"
     CDS             5483..6802
                     /locus_tag="JNC24_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HAY9158647.1"
                     /translation="MTAELRNLPHIASMAFNEPLMLEPAYARVFFCALAGQLGISCLT
                     DAVSGDSLTAGEAPAALALSGDDDGPRQARSYQVMNGIAVLPVSGTLVSRTRALQPYS
                     GMTGYNGIIARLQQAASDPMVDGILLDMDTPGGMVAGAFDCADIIARVRDIKPVWALA
                     NDMNCSAGQLLASAASRRLVTQTARTGSIGVMMAHSNYGAALEKQGVEITLIYSGSHK
                     VDGNPYSHLPDDVRETLQSRMDATRRMFAQKVSAYTGLSVQAVLDTEAAVYSGQEAID
                     AGLADELVNSTDAITVMRDALDARKSRLSGGRMTKETQSTTVSATASQADVTGVVQAT
                     EGENASAAQPDVNAQITAAVAAENSRIMGILNCEEAHGREEQARVLAETPGMTVETAR
                     RILAAAPQSAQTRSDTALDRLMQGAPAPLAAGNPASDAVNDLLNTPV"
     gene            6812..7144
                     /locus_tag="JNC24_001897"
     CDS             6812..7144
                     /locus_tag="JNC24_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HAY9158648.1"
                     /translation="MTSKETFTHYQPLGNSDPAHTATAPGGLSAKAPAMTPLMLDTST
                     RKLVAWDGTTDGAAVGILAVAADQTSTTLTFYKSGTFRYEDVLWPEAASDETKKRTVF
                     AGTAISIV"
     gene            7200..8225
                     /locus_tag="JNC24_001898"
     CDS             7200..8225
                     /locus_tag="JNC24_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HAY9158649.1"
                     /translation="MSMYTTAQLLAANEQKFKFDPLFLRLFFRESYPFTTEKVYLSQI
                     PGLVNMALYVSPIVSGEVIRSRGGSTSEFTPGYVKPKHEVNPQMTLRRLPDEDPQNLA
                     DPAYRRRRIIMQNMRDEELAIAQVEEMQAVSAVLKGKYTMTGEAFDPVEVDMGRSAAN
                     NITQSGVTEWSKRDKSTYDPTDDIEAYALNASGVVNIIVFDPKGWALFRSFKAVKEKL
                     DTRRGSHSELETAVKDLGKAVSYKGMYGDVAIVVYSGQYVENGVKKNFLPDNTMVLGN
                     TQARGLRTYGCIQDADAQREGINASARYPKNWVTTGDPAREFTMIQSAPLMLLADPDE
                     FVSVQLA"
     gene            8267..8665
                     /locus_tag="JNC24_001899"
     CDS             8267..8665
                     /locus_tag="JNC24_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HAY9158650.1"
                     /translation="MTKDELIARLQELGEQLNRDVSLTGTKEELALRVAELEEELDDT
                     DETAGQDTPLSRENVLTGHENEVVSAQPDTVIQDTAELVTVVALVTLHTDALHATRDK
                     PVAFVLPGTAFRVSAGVAAEMTERGLARMQ"
     gene            8677..9030
                     /locus_tag="JNC24_001900"
     CDS             8677..9030
                     /locus_tag="JNC24_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY9158651.1"
                     /translation="MADFDNLFDAAIARADETIRGYMGTSATMTSGEQSGAVIRGVFD
                     DPENISYAGQGVRVEGSSPSLFVRTDEVRQLRRGDTLTIGEENFWVDRVSPDDGGSCH
                     LWLGRGVPPAVNRRR"
     gene            9042..9620
                     /locus_tag="JNC24_001901"
     CDS             9042..9620
                     /locus_tag="JNC24_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY9158652.1"
                     /translation="MAIKGLEQAVENLSRISKTAVPGAAAMAINRVASSAISQSASQV
                     ARETKVRRKLVKERARLKRATVKNPQARIKVNRGDLPVIKLGNARVVLSRRRRHKKGQ
                     RSSLKGGGSVLVVGNRRIPGAFIQQLKNGRWHVMQRVAGKNRYPIDVVKIPMAVPLTT
                     AFKQNIERIRRERLPKELGYALQHQLRMVIKR"
     gene            9617..10012
                     /locus_tag="JNC24_001902"
     CDS             9617..10012
                     /locus_tag="JNC24_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY9158653.1"
                     /translation="MKHTELRAAVLDALEKHDTGATLFDGRPAVFDEEDFPAIAVYLT
                     GAEYTGEELDSDTWQAELHIEVFLPAQVPDSELDSWMESRIYPVMSDIPALSDLITSM
                     VASGYDYRRDDDAGLWSSADLTYVIIYEM"
     gene            10020..10760
                     /locus_tag="JNC24_001903"
     CDS             10020..10760
                     /locus_tag="JNC24_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY9158654.1"
                     /translation="MPVPNPTMPVKGAGTTLWVYKGSGDPYANPLSDVDWSRLAKVKD
                     LTPGELTAESYDDSYLDDEDADWTATGQGQKSAGDTSFTLAWMPGEQGQQALLAWFNE
                     GDTRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGRPSMAEDRSTVTA
                     TTGMTVTPASASVVKGQSTTLTVAFQPDGATDKSFRAVSADKTKATVSVSGMTITVKG
                     VAAGKVNIPVVSGNGEFAAVAEINVTAS"
     gene            10776..11198
                     /locus_tag="JNC24_001904"
     CDS             10776..11198
                     /locus_tag="JNC24_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HAY9158655.1"
                     /translation="MFLKTESFEHNGVTVTLSELSALQRIEHLDLMKRQAEQAESDSN
                     RKFTVEDAIRTGAFVVAMSLWHNHPKKTQMPSMNEAVKQIEQEVLTTWPTEAISHAEN
                     VVYRLSGMYEFVVNDAPEQAEDAGPAEPVSAGKCSTVS"
     gene            11180..11614
                     /locus_tag="JNC24_001905"
     CDS             11180..11614
                     /locus_tag="JNC24_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HAY9158656.1"
                     /translation="MFDGELSFALKLAREMGRPDWRAMLAGMSSTEYADWHRFYSTHY
                     FHDVLLDMHFSGLTYTVLSLFFSDPEMHPLDFSLLNRREADEEPEDDVLMQKAAGLAG
                     GVRFGPDGNEVIPASPDVADMTEDDVMLMTVSEGIAGGVRYG"
     gene            11607..14168
                     /locus_tag="JNC24_001906"
     CDS             11607..14168
                     /locus_tag="JNC24_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAY9158657.1"
                     /translation="MAEPVGDLVVDLSLDAARFDEQMARVRRHFSGTESDAKKTAAVV
                     EQSLSRQALAAQKAGISVGQYKAAMRMLPAQFTDVATQLAGGQNPWLILLQQGGQVKD
                     SFGGMIPMFRGLAGAITLPMVGATSLAVATGALAYAWYQGNSTLSDFNKTLVLSGNQA
                     GLTADRMLVLSRAGQAAGLTFNQTSESLSALVKAGVSGEAQIASISQSVARFSYASGV
                     EVDKVAEAFGKLTTDPTSGLTAMARQFHNVTAEQIAYVAQLQRSGDEAGALQAANEAA
                     TKGFDDQTRRLKENMGTLETWADRTARAFKSMWDAVLDIGRPDTAQEMLIKAEAAFKK
                     ADDIWNLRKDDYFVNDEARARYWDDREKKRLERDAAQKRVDQQRQQDKNAQQQSDTEA
                     SRLKYTEEAQKAYERLQTPLEKYTARQEELNKALKDGKILQADYNTLMAAAKKDYEAT
                     LKKPKQSGVKVYAGDRQEDSAHAALLTLQAELRTLEKHAGANEKISQQRRDLWKAESQ
                     FAVLEEAAQRRQLSAQEKSLLAHKDETLEYKRQLAALGDKVTYQEHLNALAQQADKFA
                     QQQRAKRAAIDAKNRGLTDRQAAREATEQRLKEQYGDNPLALNNVMSEQKKTWAAEDQ
                     LRGSWMAGLKSGWSEWEESATDSMSQVKSAATQTFDGIAQNMAAMLTGSEQNWRSFTR
                     SVLSMMTEILLKQAMVGIVGSIGSAIGGAVGGGASASGGTAIQAAAVKFHFATGGFTG
                     TGGKYEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYAEGGYVGGAGSPAQMRRAEG
                     INFNQNNHVVIQNDGTNGLPGPQMMKAVYDMARKGARDEIQTQMRDGGLFSGGGR"
     gene            14165..14494
                     /locus_tag="JNC24_001907"
     CDS             14165..14494
                     /locus_tag="JNC24_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY9158658.1"
                     /translation="MKTFRWKVKPGMDVASAPSVRKVRFGDGYSQRAPAGLNANLKTY
                     SVTLSVPREEAMVLESFLEEHGGWKAFLWTPPYEWRQIKVTCAKWSSRVSMLRVEFSA
                     EFEQVVN"
     gene            14494..15192
                     /locus_tag="JNC24_001908"
     CDS             14494..15192
                     /locus_tag="JNC24_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HAY9158659.1"
                     /translation="MQDIRQETLNECTRAEQSASVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQPYPIQGSGFELNGKGTSTRPTLMVSNLYGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDG
                     AVFPGRIMLANTCTWTYRGDECGYHGPAVADEYDQPTSDITKDKCSKCLSGCKFRNNV
                     GNFGGFLSINKLSQ"
     gene            15198..15941
                     /locus_tag="JNC24_001909"
     CDS             15198..15941
                     /locus_tag="JNC24_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY9158660.1"
                     /translation="MTETESAILAHARRCAPAESCGFVVRTPEGERYFPCVNISGEPE
                     AYFRMSPEDWLQAEMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGAIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHREDDWWRHGQNLYLDNLEAT
                     GLYQVPLSAAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHRAWHASAFTGICNDLAAASTFV"
     gene            15878..16510
                     /locus_tag="JNC24_001910"
     CDS             15878..16510
                     /locus_tag="JNC24_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HAY9158661.1"
                     /translation="MARICLYGDLQRFGRRIDLRVKTGAEAIRAMATQLPAFRQKLSD
                     GWYQVRIAGQDVSTSGLTAQLHEALPDGAVIHIVPRVAGAKSGGVFQIVLGAAAIAGS
                     FFTAGATLAAWGAAIGAGGMTGILFSLGASMVLGGVAQMLAPKARTPRTQTTDNGKQN
                     TYFSSLDNMVAQGNVMPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            16571..20068
                     /locus_tag="JNC24_001911"
     CDS             16571..20068
                     /locus_tag="JNC24_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HAY9158662.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVEGPVDGLKS
                     VLLNSTPVLDSEGNTNISGVTVVFRAGEQEQSPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVDNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTLKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGSTEPRITCNAWLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRQSDKVWAYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNAVEVNWIDPDNGWETATELVEDTQAIVRYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGIRTGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGQGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTL
                     HQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDQSGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVTADDGSERLVSTARTTETTYRFTQ
                     LALGNYRLTVRAVNAWGQQGDPASVSFRIAAPAALSRIELTPGYFQITATPHLAVYDP
                     TVQFEFWFSEKRIADIRQVETTARYLGTALYWIAASINIKPGHDYYFYIRSVNTVGKS
                     AFVEAVGQPSDDASGYLDFFKGEIGKTHLAQELWTQIDNGQLAPDLAEIRTSITDVSN
                     EITQTVNKKLEDQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMQDGRLYIAGIGAGIEN
                     TPDGMQSQVLLAADRIAMVNPANGNTKPMFVGQGDQIFMNDVFLKRLTAPTITSGGNP
                     SAFSLTPDGKLTAKNADISGSVNANAGTLNNVTVNENCTIKGMLEATQVRGDFVKAVS
                     KSFPKQAGTWGNTETPNGTVTVTISDDHNFDRQIIIPPIIFNGIAYDDPGSGNNPGGT
                     RYTGYGFEVRKNGVLIASRETKGAIPGSYSAVIDMPSGGGSVTLEFKIFQKGNQGAGN
                     ITDCTVIVTKKAASGISIR"
     gene            20139..20655
                     /locus_tag="JNC24_001912"
                     /pseudo
     CDS             20139..20655
                     /locus_tag="JNC24_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309676.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
     gene            20720..23803
                     /locus_tag="JNC24_001913"
     CDS             20720..23803
                     /locus_tag="JNC24_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY9158663.1"
                     /translation="MAVKISGVLKDGTGKPVQNCTIQLKAKRNSTTVVVNTLASENPD
                     EAGRYSMDVEYGQYSVILLVEGFPPSHAGTITVYEDSRPGTLNDFLGAMTEDDARPEA
                     LRRFELMVEEVARNASAVAQNTAAAKKSASDASTSAREAATHATDAADSARAASTSAG
                     QAASSAQSASSSAGTASTKATEASKSAAAAESSKSAAATSAGAAKTLETNAAASQQSA
                     ATSASTATTKASEAATSARDASASKEAAKSSETNASSSASSAASSATAAANSAKAAKT
                     SETNARSSETAAGQSASAAAGSKTAAASSASAASTSAGQASASATAAGKSAESAASSA
                     STATTKAGEATEQATAAARSASAAKTSETNAKASETRAESSKTAAASSASSAASSASS
                     ASASKDEATRQASAAKGSATTASTKATEAAGSATAAAQSKSTAESAATRAETAAKRAE
                     DIASAVALEDASTTKKGIVQLSSATNSTSESLAATPKAVKAAYDLANGKYTAQDATTA
                     QKGIIQLSSATNSTSETLAATPKAVKAANDNAEKRLQKDQNGADIPGKDTFTKNIGAC
                     RAFGGSVSTTTGNWTTAQFIEWLDSQGAFNHPYWMCKGSWSYGNNKIITDTGCGNIHL
                     AGAVIEVMGIKSAMTIRITTPTTSSGGGTTNAQFTYINHGTDYSPGWRRDYNSRNKPT
                     ASEIGALPSGGTAVSSVNLASKGRVAALTDNTQGAAGLELYEVYNNGYPTAYGNIIHL
                     KGMTAVGEGELLIGWSGTSGAHAPAFIRSRRDTTDANWSPWAQLYTSAHPPAEFYPVG
                     APIPWPSDTVPSGYALMQGQTFDKSAYPKLAAAYPSGVIPDMRGWTIKGKPASGRAVL
                     SQEQDGIKSHTHSASASSTDLGTKTTSSFDYGTKSTNNTGAHTHSLSGSTSSAGAHQH
                     SQTGPRTNSGSQPTGMFPAGSTQVSGTNQVGISGSLTSGTSQWVGKSSSEGNHTHSLS
                     GTAASAGAHAHTVGIGAHTHSVAIGSHGHTITVNAAGNAENTVKNIAFNYIVRLA"
     gene            23803..24384
                     /locus_tag="JNC24_001914"
     CDS             23803..24384
                     /locus_tag="JNC24_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HAY9158664.1"
                     /translation="MAFRMSEQARTIKIYNLLAGTNEFIGEGDAYIPPHTGLPANSTD
                     IAPPDIPAGFVAVFNSDESSWHLVEDHRGKTVYDVASGDALFISELGSLPENVTWLSP
                     EGEFQKWNGTAWVKDTEAEKLFRIREAEETKNSLMQVASEHIAPLQDAVDLEIATEKE
                     TLLLEAWKKYRVLLNRVDTSTAPDIEWPVAPIG"
BASE COUNT         5971 a         6170 c         7523 g         5297 t
ORIGIN      
        1 gtcgccgtat tcccgtatta acagagaccg tagcccgacg gggaactcct tctgcgcgag
       61 tgtgcgggaa taatcaaaaa cgatgcacac cggggttacc gggtacacat atttcatcat
      121 gccagcgagt ccggttctgg cacggaagaa accggacgtt atgatttagt gcggaaatat
      181 ttgtgtagtg ttctgtatgt tctcagtaaa gagtaatgaa ttatcaaagg tatagtaata
      241 ccttttgttt tcgtggatat ttgtaatcca tctgaaaacc cctgctgtag caagattttt
      301 cctgtattcg taaaatgata actctcctga tttgaatcct tttaaggtgg cttctataag
      361 gcatttattt tttgaaaatc ttacatttac aaccttaccc tgtcctttta ttaaaaccgt
      421 attatcgttt tcaagaacaa gatgaatatt ctctgtggct aaatagtaaa tgtaatgtga
      481 gacattgtga cgttttagtt cagaataaaa ccagtgatag tttaaattat ttcgcacttt
      541 atcgaatatt tgtttaaaaa tggcaacctg agccattgta gtaccttcca tgtgatatga
      601 gggggcgtag tctgcacgat tatctaaatt gcttcaatct ggtctaacct gttttctgag
      661 caattcagta atgtcactct tttctttgtt tgcttcaggc gaaactcttt tttctgagca
      721 cagtctccgg cggcaggctt caatgaccca ggctgagaaa ttcccggacc ctttttgaac
      781 aagagcgatg ttaatttgtt caatcatttg gttaggaaag cggatgttgc gggttgttgt
      841 tctgcgggtt ctgttcttcg ttgacatgag gttgccctgt attcagtgtc gctgatttgt
      901 attgtctgaa gttgttttta cgttaagttg atgcagatca attaatacga tacctgcgtc
      961 ataattgatt atttgacgtg gtttgatggc gtagatgcac gttgtgacat gtagatgata
     1021 attattatca ttttgcgggt cctttccggc gatccgacag gttacggggc ggaaggcgcg
     1081 cgggttttcg ctatttatga caattttccg gtttaaggcg tttccgttct tcttcgccgt
     1141 aacctaatgt ttttatttaa aacaccccct gaaaagaaag gaaacgacag gtgctgaaaa
     1201 cgggcttttt ggcctctgtc gtttcctttc tctgtttttg tccgtggaat gaacaatgga
     1261 agtcaacaaa aagcagctgg ctgacatttt cggtgcgagt atccgtacca ttcagaactg
     1321 gcaggaacag ggaatgcccg ttctgcgagg cggtggcaag ggtaatgagg tgctttatga
     1381 ctctgccgcc gttataagat ggtatgccga aagggatgct gaaattgaga acgaaaagct
     1441 gcgccgggaa gttgaagaac tgcggcaggc cagcgagaca gatctccagc cagggactat
     1501 tgagtacgaa cgccatcgac ttacgcgtgc gcaggccgac gcacaggagc tgaaaaatgc
     1561 cagagactcc gctgaagtgg tggaaaccgc attctgtact ttcgtgctgt cgcggatcgc
     1621 aggtgaaatt gccagtattc tcgacgggat ccccctgtcg gtgcagcggc gttttccgga
     1681 actggaaaac cgacatgttg atttcctgaa acgggatatc atcaaagcca tgaacaaagc
     1741 agccgcgctg gatgaactga taccggggtt gctgagtgaa tatatcgaac agtcaggtta
     1801 acaggctgcg gcattttgtc cgcgccgggc ttcgctcact gttcaggccg gagccacaga
     1861 ccgccgttga atgggcggat gctaattact atctcccgaa agaatccgca taccaggaag
     1921 ggcgctggga aacactgccc tttcagcggg ccatcatgaa tgcgatgggc agcgactaca
     1981 tccgtgaggt gaatgtggtg aagtctgccc gtgtcggtta ttccaaaatg ctgctgggtg
     2041 tttatgccta ctttatagag cataagcagc gcaacacact tatctggttg ccgacggatg
     2101 gtgatgccga gaactttatg aaaacccacg ttgagccgac catccgcgat attccgtcgc
     2161 tgctggcgct ggctccgtgg tatggcaaaa agcaccggga taacacgctc actatgaagc
     2221 gtttttccaa tggtcgtggc ttctggtgcc tgggcggtaa agcggcaaaa aactaccgtg
     2281 aaaagtcggt ggatgtggcg ggttatgatg aacttgctgc ctttgatgag gatattgaac
     2341 aggaaggctc tccgacgttc cttggcgaca aacgtattga aggctcggtc tggccaaagt
     2401 ccatccgtgg ctccacgccc aaagtgagag gcacctgcca gattgagcgt gcagccagtg
     2461 aatccccgca ttttatgcgt tttcatgttg cctgcccgca ctgcggggag gagcagtatc
     2521 ttaaatttgg cgacaaagag acgccgtttg gcctcaaatg gacgccggat gacccctcca
     2581 gcgtgtttta tctctgcgag cataatgcct gcgtcatccg ccagcaggag ctggacttta
     2641 ctgatgcccg ttatatctgc gaaaagaccg ggatctggac ccgtgatggc attctctggt
     2701 tttcgtcatc cggtgaagag attgagccac ctgacagtgt gacctttcac atctggacag
     2761 cgtacagccc gttcaccacc tgggtgcaga ttgtcaaaga ctggatgaaa acgaaagggg
     2821 atacgggaaa acgtaaaacc ttcgtaaaca ccacgctcgg tgagacgtgg gaggcgaaaa
     2881 ttggcgaacg tccggatgct gaagtgatgg cagagcggaa agagcattat tcagcgcccg
     2941 ttcctgaccg tgtggcttac ctgaccgccg gtatcgactc ccagcgggat cgctacgaaa
     3001 tgcgcgtatg gggatggggg ccgggtgagg aaagctggct gattgatcgg cagattatta
     3061 tgggccgcca cgacgatgaa cagacgctgc tgcgtgtgga tgaggccatc aataaaacct
     3121 atacccgccg gaatggtgca gaaatgtcga tatcccgtat ctgctgggat attggcggga
     3181 ttgacccgac cattgtgtat gaacgctcga aaaagcatgg gctgttccgg gtgatcccca
     3241 ttaaaggggc atccgtctac ggtaagccgg tggccagcat gcctcgtaag cgaaacaaaa
     3301 acggggttta ccttaccgaa atcggtacgg ataccgcgaa agagcagatt tataaccgct
     3361 tcacactgac gccggaaggg gatgaaccgc ttcccggtgc cgttcacttc ccgaataacc
     3421 cggatatttt tgatctgacc gaagcgcagc agctggctgc tgaagagcag gtcgaaaaat
     3481 gggtggatgg caggaaaaaa atactgtggg acagcaaaaa gcgacgcaat gaggcgctcg
     3541 actgcttcgt ttatgcgctg gcggcgctgc gcatcagtat ttcccgctgg cagctggatc
     3601 tcagtgcact gctggcgagc ctgcaggaag aggatggtgc agcaaccaac aagaaaacac
     3661 tggcagatta cgcccgtgcc ttatccggag aggatgaatg acgcgacagg aagaacttgc
     3721 cgctgcccgt gcggcactgc atgacctgat gacaggtaaa cgggtggcaa cggtacagaa
     3781 agacggacgg cgagtggagt ttacggccac ttccgtgtct gacctgaaaa aatacattgc
     3841 ggagctggag gtgcagaccg gcatgacaca gcgacgcagg ggacctgcag gattttatgt
     3901 atgaaaacgc ccaccattcc cacccttctg ggaccggacg gcatgacatc gctgcgtgaa
     3961 tatgccggtt atcacggcgg tggcagcgga tttggtgggc agttgcgggc gtggaaccca
     4021 ccgggtgaaa gtgtggatgc agccctgctg cccaacttta cccgtggcaa tgcccgcgca
     4081 gacgatctgg tacgcaataa cggctatgct gccaacgcca tccagctgca tcaggatcat
     4141 atcgtcgggt cttttttccg gctcagtcat cgcccaagct ggcgctatct gggcatcggg
     4201 gaggaagacg cccgtgcctt ttcccgcgag gttgaagcgg catggaaaga gtttgccgag
     4261 gatgactgct gctgcattga cgttgagcga aaacgcacgt ttaccatgat gattcgggaa
     4321 ggtgtggcca tgcacgcctt taacggtgaa ctgttcgttc aggccacctg ggataccagt
     4381 tcgtcgcggc ttttccggac acagttccgg atggtcagcc cgaagcgcat cagcaacccg
     4441 aacaataccg gcgacagccg gaactgccgt gccggtgtgc agattaatga cagcggtgcg
     4501 gcgctgggat attacgtcag cgaggacggc tatcctggct ggatgccgca gaaatggaca
     4561 tggatacccc gtgagttacc cggcgggcgc gcctcgttca ttcacgtttt tgaacccgtg
     4621 gaggacgggc agacccgcgg tgcaaatgtg ttttacagcg taatggagca gatgaagatg
     4681 ctcgacacgc tgcagaacac gcagctgcag agcgccattg tgaaggcgat gtatgccgcc
     4741 accattgaaa gtgagctgga tacgcagtca gcgatggatt ttattctggg cgcgaacagt
     4801 aaggagcagc gggaaaggct gacgggctgg attggtgaaa ttgccgcgta ttacgccgca
     4861 gcaccggtcc gtctgggagg cgcaaaagtg ccgcacctga tgccggggga ctcactgaac
     4921 ctgcagacgg ctcaggacac ggataacggc tactccgtgt ttgagcagtc actgttgcgg
     4981 tatatcgctg ccgggctggg tgtctcgtat gagcaacttt cccggaatta cgcccagatg
     5041 agctactcca cggcacgggc cagtgcgaac gagtcgtggg cgtactttat ggggcggcga
     5101 aaattcgtcg catcccgtca ggcgagccag atgtttctgt gctggctgga agaggccatc
     5161 gttcgccgcg tggtgacgtt accttcaaaa gcgcgcttca gctttcagga agcccgcagt
     5221 gcctggggga actgcgactg gataggctcc ggtcgtatgg ccatcgatgg tctgaaagaa
     5281 gttcaggaag cggtgatgct gatagaagcc ggactgagca cctacgagaa agagtgcgcg
     5341 aaacgcggtg acgactatca ggaaattttt gcccagcagg tccgtgaaac gatggagcgc
     5401 cgcgcagctg gtcttaaacc gcccgcctgg gcggctgcgg catttgaatc cgggctgcga
     5461 caatcaacag aggaggagaa gagtgacagc agagctgcgt aatctcccgc atattgccag
     5521 catggctttt aatgagccgc tgatgcttga acccgcctat gcgcgggttt tcttttgtgc
     5581 gcttgcaggc cagcttggga tcagctgcct gacggatgca gtatccggcg acagcctgac
     5641 tgccggagag gcacccgcgg cgctggcgtt atccggtgat gatgacggac cacgacaggc
     5701 ccgcagttat caggtaatga acggcatcgc cgtgctgccg gtgtccggca cgctggtcag
     5761 ccggacgcgg gcgctgcagc cgtactcggg gatgaccggt tacaacggca ttatcgcccg
     5821 tctgcaacag gctgccagcg acccgatggt ggacggcatt ctgctcgata tggacacacc
     5881 gggcggaatg gtggcgggag catttgactg cgctgacatc atcgcccgtg tgcgtgacat
     5941 aaagccggta tgggcgctgg ccaatgacat gaactgcagc gcaggtcagc tgcttgccag
     6001 tgccgcctcc cggcgtctgg tcacgcagac cgcccggaca ggctccatcg gcgtcatgat
     6061 ggctcacagt aattacggcg ctgcactgga gaaacagggc gtggaaatca cgctgattta
     6121 cagcggcagc cataaggtgg atggcaaccc ctacagccat ctaccggatg atgtccggga
     6181 aacattgcag tcccggatgg atgcaacccg ccggatgttt gcacagaagg tgtcggcata
     6241 taccggcctg tccgtgcagg ctgtgctgga taccgaggct gcagtgtaca gcggtcagga
     6301 ggccattgat gccggactgg ctgatgaact tgttaacagt accgatgcga tcaccgtcat
     6361 gcgtgatgca ctggatgcac gtaaatcccg tctctcagga gggcgaatga ccaaagagac
     6421 tcaatcaaca actgtttcag ccactgcttc gcaggctgac gttactggcg tggtgcaagc
     6481 gacggagggc gagaacgcca gcgcggcgca gccggacgtg aacgcgcaga tcaccgcagc
     6541 ggttgcggca gaaaacagtc gcattatggg gatcctcaac tgtgaggagg ctcacggacg
     6601 cgaagaacag gcacgcgtgc tggccgaaac ccccggtatg accgtggaaa cggcccgccg
     6661 tattctggcc gcagcaccac agagtgcaca gacgcgcagt gacactgcgc tggatcgtct
     6721 gatgcagggg gcaccggcac cgctggctgc aggtaacccg gcatctgatg ccgttaacga
     6781 tttgctgaac acaccagtgt aagggatgtt tatgacgagc aaagaaacct ttacccatta
     6841 ccagccgctg ggcaatagtg acccggctca taccgcaacc gcgcccggcg gattgagtgc
     6901 gaaagcgcct gcaatgaccc cgctgatgct ggacacctcc acccgtaagc tggttgcgtg
     6961 ggatggcacc accgacggtg ctgccgttgg cattcttgcg gttgctgctg accagaccag
     7021 caccacactg acgttctaca agtccggcac gttccgttat gaggatgtgc tctggccgga
     7081 ggctgccagc gacgagacga aaaaacggac cgtgtttgcc ggaacggcaa tcagcatcgt
     7141 ttaactttac ccttcatcac taaaggccgc ctgtgtggct ttttttacgg gattttttta
     7201 tgtcgatgta cacaaccgcc cagctgctgg cggcaaatga gcagaaattt aagtttgatc
     7261 cgctgtttct gcgtctcttt ttccgtgaga gctatccctt cactacggag aaagtctatc
     7321 tctcacaaat tccgggactg gtaaacatgg cgctgtacgt ttcgccgatt gtttccggtg
     7381 aggttatccg ttcccgtggc ggctccacct ctgaatttac gccgggatat gtcaaaccca
     7441 agcatgaggt gaatccgcag atgaccctgc gtcgcctgcc ggatgaagat ccgcagaatc
     7501 tggcggaccc ggcttaccgc cgccgtcgca tcatcatgca gaacatgcgt gacgaagagc
     7561 tggccattgc tcaggtcgaa gagatgcagg cagtttctgc cgtgctcaag ggcaaataca
     7621 ccatgaccgg tgaagccttc gatccggttg aggtggatat gggccgcagt gcggcgaaca
     7681 acatcacaca gtccggcgtc acggagtgga gcaagcgtga caagtccacg tatgacccga
     7741 ccgacgatat cgaagcctac gcgctgaacg ccagcggtgt ggtgaatatc atcgtgtttg
     7801 acccgaaagg ctgggcgctg ttccgttcct tcaaagccgt caaggagaag ctggataccc
     7861 gtcgcggctc tcattccgag ctggagacag cggtaaaaga cctgggcaaa gcggtgtcct
     7921 ataagggaat gtatggcgat gtggccatcg tcgtgtattc cggacagtac gtggaaaacg
     7981 gcgtcaaaaa gaacttcctg ccggacaaca cgatggtgct ggggaacact caggctcgcg
     8041 gtctgcgcac ctatggctgc attcaggatg cggacgcaca gcgcgaaggc attaacgctt
     8101 ctgcccgtta cccgaaaaac tgggtgacca ccggcgatcc ggcgcgtgag ttcaccatga
     8161 ttcagtcagc accgctgatg ctgctggctg atcctgatga gttcgtgtcc gttcaactgg
     8221 cgtaatcatg gcccttcggg gccattttct ctctgtggag gagttcatga cgaaagatga
     8281 actgattgcc cgtcttcagg agctgggtga gcaattgaac cgcgatgtca gtttgacggg
     8341 aacgaaagaa gaactggcgc ttcgtgtggc agagctggaa gaagagcttg atgacacgga
     8401 tgaaactgcc ggtcaggaca cccctctcag ccgggaaaat gtgctgaccg ggcatgaaaa
     8461 tgaggtggta tcagcgcagc cggataccgt gattcaggat acggctgaac tggtcacggt
     8521 cgtggcactg gtgacgctgc atactgatgc acttcacgcc acgcgggata aacctgtggc
     8581 atttgtgctg ccgggaacgg cgtttcgtgt ctctgccggt gtggcagccg aaatgacaga
     8641 gcgcggcctg gccagaatgc aataacggga ggcgctgtgg ctgatttcga taacctgttc
     8701 gatgctgcca ttgcccgcgc cgatgaaacg atacgcgggt acatgggaac gtcagccacc
     8761 atgacatccg gtgagcagtc aggtgcggtg atacgtggtg tttttgatga ccctgaaaat
     8821 atcagctatg ccggacaggg cgtgcgcgtt gaaggctcca gcccgtccct gtttgtccgg
     8881 actgatgagg tgcggcagct gcggcgtgga gacacgctga ccatcggtga ggaaaatttc
     8941 tgggtagatc gggtttcgcc ggatgatggc ggaagttgtc atctctggct tggacggggc
     9001 gtaccgcctg ccgttaaccg tcgccgctga aagggggatg tatggccata aaaggtcttg
     9061 agcaggccgt tgaaaacctc agccgtatca gcaaaacggc ggtgcccggt gccgccgcaa
     9121 tggccattaa ccgcgttgct tcatccgcga tatcgcagtc tgcgtcacag gttgcccgtg
     9181 agacaaaggt acgccggaaa ctggtaaagg aaagggccag gctgaaaagg gccacggtca
     9241 aaaatccgca ggccagaatc aaagttaacc ggggggattt gcccgtaatc aagctgggta
     9301 atgcgcgggt tgtcctgtcc cgccgcaggc gtcataaaaa ggggcagcgt tcatccctga
     9361 aaggtggcgg cagcgtgctt gtggtgggta accgtcgtat tcccggcgcg tttattcagc
     9421 aactgaaaaa cggccggtgg catgttatgc agcgtgtggc cgggaaaaac cgttacccca
     9481 ttgatgtggt gaaaatcccg atggcggtgc cgctgaccac ggcgtttaaa cagaatattg
     9541 aacggatacg gcgtgaacgt cttccgaaag agctgggcta tgcgctgcag catcaactga
     9601 gaatggtaat aaagcgatga aacatactga actccgtgca gccgtactgg atgcactgga
     9661 gaagcatgac accggggcga cgctttttga tggtcgcccc gctgtttttg atgaggaaga
     9721 ttttccggca attgccgttt atctcaccgg cgctgaatac acgggcgaag agctggacag
     9781 cgatacctgg caggcggagc tgcatattga agttttcctg cctgctcagg tgccggattc
     9841 agagctggat tcgtggatgg agtcccggat ttatcctgtg atgagcgata tcccggcact
     9901 gtcagatttg atcaccagta tggtggccag cggctatgac taccggcgcg acgatgatgc
     9961 gggcctgtgg agttcagccg atctgactta tgtcattatc tatgaaatgt gaggacgcta
    10021 tgcctgtacc aaatcctaca atgccggtga aaggtgccgg gaccaccctg tgggtttata
    10081 aggggagcgg tgacccttat gcgaacccgc tttcagacgt tgactggtcg cgtctggcaa
    10141 aagttaaaga cctgacgccc ggcgaactga ccgctgagtc ctatgacgac agctatctcg
    10201 atgatgaaga tgcagactgg actgcgaccg ggcaggggca gaaatctgcc ggagatacca
    10261 gcttcacgct ggcgtggatg cccggagagc aggggcagca ggcgctgctg gcgtggttta
    10321 atgaaggtga tacccgtgcc tataaaatcc gcttcccgaa cggcacggtc gatgtgttcc
    10381 gtggctgggt cagcagtatc ggtaaggcgg tgacggcgaa ggaggtgatt acccgcacgg
    10441 tgaaggtcac caatgtggga cgtccgtcga tggcagaaga tcgcagcacg gtgacggcga
    10501 cgaccggcat gacggtgaca cccgccagtg cttccgtagt gaaagggcag agcaccacgc
    10561 tgaccgtggc attccagccg gatggcgcaa ccgacaagag cttccgtgcg gtgtctgcgg
    10621 ataaaacaaa agccaccgtg tcggtcagtg gtatgaccat caccgtgaaa ggtgttgctg
    10681 caggcaaggt caacattccg gtcgtatctg gtaatggtga gtttgctgcg gttgcagaaa
    10741 tcaacgtcac cgccagttaa tccggagagt cagcgatgtt cctgaaaacc gaatcatttg
    10801 aacataacgg tgtgaccgtc acgctttctg aactgtcagc cctgcagcgt attgagcatc
    10861 tcgacctgat gaaacggcag gcagaacagg cggagtcaga cagcaaccgg aagtttactg
    10921 tggaagacgc catcagaacc ggcgcttttg tggttgcgat gtccctgtgg cataaccatc
    10981 cgaagaagac gcagatgccg tccatgaatg aagccgttaa acagattgag caggaagtgc
    11041 ttaccacctg gcccacagag gcaatttctc atgctgaaaa cgtggtgtac cggctgtccg
    11101 gtatgtatga gtttgtggtg aatgatgctc ctgaacaggc agaggacgcc gggcctgcag
    11161 agcctgtttc tgcgggaaag tgttcgacgg tgagctgagt tttgccctga aactggcgcg
    11221 tgagatgggg cgacccgact ggcgcgccat gcttgccggg atgtcatcca cggagtatgc
    11281 cgactggcac cgcttttaca gtacccatta ttttcatgat gttctgctgg atatgcactt
    11341 ttccgggctg acgtacaccg tgctcagcct gtttttcagc gatccggaga tgcatccgct
    11401 ggatttcagt ctgctgaacc ggcgcgaggc tgacgaagag cctgaagatg atgtgctgat
    11461 gcagaaagcg gcagggcttg ccggaggtgt ccgctttggc ccggacggga atgaagttat
    11521 ccccgcttcc ccggatgtgg cggacatgac ggaggatgac gtaatgctga tgacagtatc
    11581 agaagggatc gcaggaggag tccggtatgg ctgaaccggt aggcgatctg gtcgttgatt
    11641 tgagtctgga tgcggccaga tttgacgagc agatggccag agtcaggcgt catttttccg
    11701 gtacggaaag tgatgcgaaa aaaacagcgg cagtcgttga acagtcgctg agccgacagg
    11761 cgctggctgc acagaaagcg gggatttccg tcgggcagta taaagccgcc atgcgtatgc
    11821 tgcctgcaca gttcaccgac gtggccacgc agcttgcagg ggggcagaat ccctggctga
    11881 tcctgctgca acagggtggt caggttaagg actccttcgg cgggatgatc cccatgttca
    11941 gggggcttgc cggcgcgatc accctgccga tggtgggggc cacctcgctg gcggtggcga
    12001 ccggtgcgct ggcgtatgcc tggtatcagg gcaactcaac cctgtccgat ttcaacaaaa
    12061 cgctggtcct ttccggtaat caggcgggac tgacggcaga tcgtatgctg gtcctgtcca
    12121 gagccgggca ggcggcaggg ctgacgttta accagaccag cgagtcactc agcgcactgg
    12181 ttaaggcggg ggtaagcggt gaggctcaga ttgcgtccat cagccagagt gtggcgcgtt
    12241 tctcctatgc atccggcgtg gaggtggaca aggtcgctga agccttcggg aagctgacca
    12301 cagacccgac gtcggggctg acggcgatgg cacgccagtt ccataacgtg acggcggagc
    12361 agattgcgta tgttgctcag ttgcagcgtt ccggcgatga agccggggca ttgcaggcgg
    12421 cgaacgaggc cgcaacgaaa gggtttgatg accagacccg ccgcctgaaa gagaacatgg
    12481 gcacgctgga gacctgggca gacaggactg cacgtgcatt caaatccatg tgggatgcgg
    12541 tgctggatat tggtcgtcct gataccgcgc aggagatgct gattaaggca gaggctgcgt
    12601 ttaagaaagc agacgacatc tggaatctgc gcaaggatga ttatttcgtt aacgatgaag
    12661 cacgggcgcg ttactgggat gatcgtgaaa aaaaacgcct ggagcgggat gcagcccaaa
    12721 agagggtaga tcaacagcgt caacaggaca aaaatgcgca gcagcagagc gataccgaag
    12781 cgtcacggct gaaatatacc gaagaggcgc agaaggctta cgaacggctg cagacgccgc
    12841 tggagaaata taccgcccgt caggaagaac tgaacaaggc actgaaagac ggaaaaatcc
    12901 tgcaggcaga ttacaacacg ctgatggcgg cggcgaaaaa ggattatgaa gcgacgctga
    12961 aaaagccgaa acagtccggc gtgaaggtgt atgcgggcga tcgtcaggaa gacagtgctc
    13021 atgcggccct gctgacgctt caggcagaac tccggacgct ggagaagcat gccggagcga
    13081 atgagaaaat cagccagcag cgccgggatt tgtggaaggc agaaagtcag ttcgcggtac
    13141 tggaggaggc ggcacaacgt cgccagctgt ccgcacagga gaaatccctg ctggcgcata
    13201 aagacgagac gctggagtac aaacgccagc tggctgcact tggtgacaag gttacgtatc
    13261 aggagcacct gaatgcgctg gcgcagcagg cggataaatt cgcacagcag caacgggcaa
    13321 aacgggcagc cattgatgcg aaaaaccggg ggctgactga ccggcaggca gcgcgggaag
    13381 ccacggaaca gcgcctgaag gaacagtatg gcgataatcc tctggcgctg aataacgtca
    13441 tgtcagagca gaaaaagacc tgggcggctg aagaccagct tcgcgggagc tggatggcag
    13501 gcctgaagtc cggctggagt gagtgggaag agagcgccac ggacagtatg tcacaggtta
    13561 aaagtgcagc cacgcagacc tttgatggta ttgcacagaa tatggcggcg atgctgaccg
    13621 gcagtgaaca gaactggcgc agcttcaccc gctccgtgct gtccatgatg acagaaattc
    13681 tgcttaagca ggcaatggtg gggattgtcg ggagtatcgg cagcgccatt ggcggggctg
    13741 ttggtggcgg cgcatccgcg tcaggtggta cagccattca ggccgctgcg gtgaaattcc
    13801 attttgcaac cgggggattt acgggaaccg gcggcaaata tgagccagcg gggattgttc
    13861 accgtggtga atttgtcttc acgaaggagg caaccagccg gattggcgtg gggaatctct
    13921 accggctgat gcgcggctat gcggaaggtg gttatgtggg cggtgccgga agtccggcgc
    13981 agatgcggcg ggctgaaggc attaatttta atcagaacaa tcacgtggtg attcagaacg
    14041 acggtacgaa tggtctgcca ggtccacaga tgatgaaggc cgtgtatgac atggcccgca
    14101 agggtgcccg tgatgaaatt cagacacaga tgcgtgatgg tggcctgttc tccggaggtg
    14161 gacgatgaaa accttccgct ggaaagtgaa acccggtatg gatgtggctt cggccccttc
    14221 tgtaagaaag gtgcgctttg gtgatggcta ttcccagcga gcgcctgccg ggctgaatgc
    14281 caacctgaaa acgtacagcg tgacgctttc tgtcccccgt gaggaggcca tggtactgga
    14341 gtcgtttctg gaagagcacg ggggctggaa agcctttctg tggacgccgc cttatgagtg
    14401 gcggcagata aaggtgacct gcgcaaaatg gtcgtcgcgg gtcagtatgc tgcgtgttga
    14461 gttcagcgca gagtttgaac aggtggtgaa ctgatgcagg atatccggca ggaaacactg
    14521 aatgaatgca cccgtgcgga gcagtcggcc agcgtggtgc tctgggaaat cgatctgaca
    14581 gaggtcggtg gagaacgtta ttttttctgt aatgagcaga acgaaaaagg tgagccggtc
    14641 acctggcagg ggcgacagta tcagccgtat cccattcagg ggagtggttt tgaactgaat
    14701 ggcaaaggca ccagtacgcg ccccacgctg atggtttcta acctgtacgg tatggtcacc
    14761 gggatggcgg aagatctaca gagtctggtc ggcggaacgg tggtccggcg taaggtttac
    14821 gcccgttttc tggatgcggt gaacttcgtc aacggaaaca gtgacgccga tccggagcag
    14881 gaggtgatca gccgctggcg cattgagcag tgcagcgaac tgagcgcggt gagtgcctct
    14941 tttgtactgt ccacgccgac ggaaacagat ggcgctgttt ttccgggacg tatcatgctg
    15001 gccaacacct gcacctggac ctatcgcggc gatgagtgcg gttatcacgg tccggcggtc
    15061 gcggatgaat atgaccagcc aacgtccgac atcacgaagg ataaatgcag caaatgcctg
    15121 agtggctgta agtttcgcaa taacgtcggc aactttggcg gcttcctttc cattaacaaa
    15181 ctttcgcagt aaatcccatg acagagacag aatcagcgat tctggcgcac gcccggcgat
    15241 gtgctccagc ggagtcgtgc ggcttcgtgg taagaacgcc ggagggggaa agatatttcc
    15301 cctgcgtgaa tatctccggt gagccggagg cgtatttccg tatgtcgccg gaagactggc
    15361 tgcaggcaga gatgcagggt gagattgtgg cactggtcca cagccacccc ggtggtctgc
    15421 cctggctgag tgaggccgac cggcggctgc aggtgcagag tgatttgccg tggtggctgg
    15481 tctgccgggg ggcgattcac aagttccgct gtgtgccaca tcttaccggg cggcgctttg
    15541 agcacggggt gacggactgt tacacgctgt tccgggatgc ttaccatctg gcggggattg
    15601 agatgccgga ttttcatcgt gaggatgact ggtggcgtca cggtcagaat ctctatctgg
    15661 ataatctgga ggccacaggg ctgtatcagg tgccgttgtc agcggcgcag ccgggcgatg
    15721 tgctgctgtg ctgttttggt tcatcggtgc cgaatcatgc cgccatttac tgtggtgacg
    15781 gcgagctgct gcaccatatt cctgaacaac tgagcaaacg agagaggtat accgacaaat
    15841 ggcagcgacg cacacactcc ctctggcgtc accgggcatg gcacgcatct gcctttacgg
    15901 ggatttgcaa cgatttggcc gccgcatcga ccttcgtgtg aaaacgggtg ccgaagccat
    15961 ccgggcgatg gccacacagc tccctgcgtt tcgtcagaag ctgagcgacg gctggtatca
    16021 ggtgcgtatt gccgggcagg atgttagcac gtccggatta acggcacagt tacatgaggc
    16081 tctgcctgac ggcgctgtga ttcatattgt tcccagagtc gccggggcca agtcaggtgg
    16141 tgtattccag attgtcctgg gagcagccgc cattgccgga tcattcttta ccgccggagc
    16201 cacccttgca gcatgggggg ctgccattgg tgccggtggt atgaccggca ttctgttttc
    16261 tctcggtgcc agtatggtgc tcggcggtgt ggctcagatg ctggcaccga aagccagaac
    16321 tccccgcaca cagacaacgg ataacggtaa gcagaacacc tatttctcct cactggataa
    16381 catggttgcc cagggcaatg ttatgcctgt tctgtatggt gaaatgcgcg tggggtcacg
    16441 tgtggtatct caggagatca gcacggcaga cgaaggggac ggtggtcagg ttgtggtgat
    16501 tggtcgctga tgcaaaatgt tttatgtgaa atcgcctccg ggcggtttta tcgtttatgg
    16561 agcatgacga atgggtaaag gcagcagtaa ggggcatacc ccgcgcgaag cgaaggacaa
    16621 cctgaagtcc acgcagctgc tgagtgtgat cgatgccatc agcgaagggc cggttgaagg
    16681 tccggtggat ggattaaaaa gcgtgctgct gaacagtacg ccggtgctgg acagtgaggg
    16741 gaataccaac atctccggcg tcacggtggt gttccgggca ggtgagcagg agcagtcacc
    16801 gccggaggga tttgaatcct ccggctccga gacggtgctg ggtacggaag tgaaatatga
    16861 cacgccgatc acccggacca tcacgtcggc aaacattgac cgactgcgtt ttaccttcgg
    16921 tgtacaggca ctggtggaaa ccacctcaaa gggagacagg aatccgtctg aagttcgcct
    16981 gctggttcag atacagcgta acggtggctg ggtgacggaa aaagacatca ccattaaggg
    17041 caaaaccacc tcgcagtatc tggcctcggt ggtggtggat aacctgccgc cgcgcccgtt
    17101 taatatccgg atgcgcagaa tgacgccgga cagcaccaca gaccagctgc agaacaaaac
    17161 gctctggtcg tcatacaccg aaatcatcga tgtgaaacag tgctacccga acacggcact
    17221 ggtcggcgta caggtggatt cggagcagtt cggcagccag caggtgagcc gtaattatca
    17281 tcttcgcggg cgcattctgc aggtgccgtc gaactataat ccgcagacgc ggcaatacag
    17341 cggtatctgg gacggaacgt taaaaccggc atacagcaac aacatggcct ggtgtctgtg
    17401 ggatatgctg acccatccgc gctatggcat ggggaaacgt cttggtgcgg cagatgtgga
    17461 taaatgggcg ctgtatgtca tcggccagta ctgtgaccag tcggtgccgg acggctttgg
    17521 cagcacggag ccgcgcatca cctgtaatgc ctggctgacc acacagcgca aggcgtggga
    17581 tgtgctcagt gatttctgct cggcgatgcg ctgtatgccg gtatggaacg ggcagacgct
    17641 gacgttcgtg caggaccgac agtcggataa ggtgtgggcc tataaccgca gtaatgtggt
    17701 gatgccggat gatggcgcgc cgttccgcta cagtttcagc gccctgaaag accgccataa
    17761 tgccgttgag gtgaactgga ttgacccgga taacggctgg gagacggcga cagagcttgt
    17821 ggaggatacg caggccattg tccgttacgg tcgtaacgtc acgaagatgg atgcctttgg
    17881 ctgtaccagc cgggggcagg cacaccgcgc cgggctgtgg ctgattaaaa cggaactgct
    17941 ggagacgcag accgtggatt tcagcgtggg tgctgaaggg cttcgtcatg taccgggcga
    18001 tgtcattgaa atctgcgatg atgactatgc cggtatccgc accggcgggc gcgtgctggc
    18061 ggtgaacagc cagacccgga cgctgacgct cgaccgtgaa atcacgctgc catcctccgg
    18121 caccacgctg ataagcctgg ttgacgggca ggggaatccg gtcagcgtgg aggtccagtc
    18181 cgtcaccgac ggcgtgaagg taaaagtgag ccgtgttcct gacggtgttg ctgaatacag
    18241 cgtgtggggg ctgaagctgc cgacgctgca ccagcgattg ttccgctgcg tgagtatccg
    18301 tgagaacgac gacggcacgt atgccatcac cgccgtgcag catgtgccgg aaaaagaagc
    18361 catcgtggat aacggggcgc actttgacgg cgaccagagc ggcacggtga atggtgtcac
    18421 gccgccagcg gtgcagcacc tgactgccga agtcaccgca gacagcgggg aatatcaggt
    18481 gctggcgcgc tgggacacgc cgaaggtggt gaagggcgtg agcttcctgc tccgtctgac
    18541 cgtaacagcg gatgacggca gtgagcggct ggtcagcacg gcccggacga cggaaaccac
    18601 ttaccgcttc acacaactgg cgctggggaa ctacaggctg acagtccggg cagtaaatgc
    18661 gtgggggcag cagggcgatc cggcgtcggt atcgttccgg attgccgcac cggcagcgct
    18721 gtcgcggatt gagctgacgc cgggctattt tcagataacc gccacgccgc atcttgcggt
    18781 ttatgatccg acggtacagt ttgagttctg gttctcggaa aagcggattg cggatatcag
    18841 gcaggttgaa accacagcac gctatcttgg cacggcgctg tactggatag ccgccagtat
    18901 caatatcaaa ccgggccatg attattactt ttatatccgc agtgtgaaca ccgttggcaa
    18961 atcggcattc gtggaggctg ttggtcagcc gagtgatgat gcatccggtt atctggattt
    19021 tttcaaaggc gagataggga aaacccatct ggctcaggag ctgtggacgc agattgataa
    19081 cggtcagctt gcgcctgacc tggctgaaat caggacgtcc attacggatg tcagcaatga
    19141 aatcacgcag accgtcaata agaaactgga agaccagagt gcggcaattc agcagataca
    19201 gaaggttcag gttgatacaa ataataacct gaacagcatg tgggctgtga agctgcagca
    19261 gatgcaggac ggacgccttt atatcgcggg tattggtgcc ggtattgaga acacccctga
    19321 cggcatgcag agtcaggtgc tgctggcggc ggacaggatt gcgatggtta atcctgcgaa
    19381 tggcaacaca aaaccgatgt ttgttggtca gggcgatcag atattcatga acgacgtgtt
    19441 cctgaaacgc ctgacggccc ccaccattac cagcggtgga aatccatcgg cattttccct
    19501 gactccggac ggaaagctga ccgctaaaaa tgcggatatc agtggcagtg tgaatgcgaa
    19561 cgccgggacg ctcaacaatg tcacggtaaa tgaaaactgt acgattaagg gcatgctgga
    19621 ggcgactcag gtcagaggtg acttcgttaa agctgtatcc aaatcatttc cgaaacaggc
    19681 tggtacgtgg ggtaacacgg aaacaccaaa cgggacggtt acagtcacca taagcgatga
    19741 tcataacttt gaccgccaga ttattattcc gcccattatt tttaacggta tagcgtatga
    19801 cgatccgggg agcggaaata acccaggagg cacgcgatac acgggttatg gttttgaagt
    19861 tcgcaaaaac ggcgtattaa tcgcatccag agaaactaaa ggtgccattc ccggtagtta
    19921 cagtgcagtt attgatatgc ctagtggtgg tggtagcgtc actctggagt ttaagatttt
    19981 ccagaaaggc aatcaggggg caggcaatat caccgactgt acggtgattg tgaccaaaaa
    20041 agcggcttcc ggcatcagta ttcgttgaaa tatttataac cccaataaat ggcgtcagga
    20101 atgacgcctt ttttattgca gaaaagcgag aggtaattat gcgtaaagtt tgtgcagcca
    20161 ttttgtccgc agccatttgc ctggccgtat ccggtgcgcc tgcatgggcg tctgaacatc
    20221 agtccacgct gagcgcgggg tatcttcatg cccggaccaa cgttcccggc agtgatgatc
    20281 tgaacgggat taacgtgaaa taccgttatg agtttacgga tacgctgggg ctggtgacgt
    20341 cattcagcta tgcaggagac aagaatcgcc agctgacccg ttacagcgat acccgctggc
    20401 atgaagattc cgtgcgtaac cgctggttca gcgtgatggc ggggccgtct gtgcgcgtga
    20461 atgaatggtt cagcgcgtat gcgatggcgg gtgtggctta cagccgtgtg tcgacttttt
    20521 ccggggatta tatccgcgta actgacaata aggggaaaac gcacgatgtg ctgaccggaa
    20581 gtgatgacgg tcgccacagc aacacgtctc tggcgcactg acggtttcat cgtgggtgtc
    20641 ggttataaat tctgattagc caggtaacac agtgttatga cagcccgccg gttcaggcgg
    20701 gcttttttgt ggggtgaata tggcagtaaa gatttcaggt gtactgaaag acggcacagg
    20761 aaaaccggta cagaactgca caatccagct gaaagcaaaa cgtaacagca ccacggtggt
    20821 ggtgaacacg ctggcctcag aaaatccgga tgaagccggg cgttacagca tggacgttga
    20881 gtacggtcag tacagcgtta ttctgttggt ggaaggattc ccgccgtcac atgccgggac
    20941 catcaccgtg tatgaagatt cccgacccgg tacgctgaat gattttctcg gtgccatgac
    21001 ggaggatgat gcccgtccgg aggcactgcg ccgttttgag ctgatggtgg aagaggtggc
    21061 gcgtaacgcg tccgcggtgg cacagaacac ggcagccgcg aagaaatcag ccagcgatgc
    21121 cagcacatca gcccgtgagg cggcaaccca tgcgactgat gctgcagatt cagcacgtgc
    21181 agccagcacg tcagccggac aggccgcttc gtcggctcag tcagcgtctt ccagcgcagg
    21241 aacggcatca acaaaggcca ctgaagcctc aaaaagtgct gccgctgcag agtcctcaaa
    21301 aagcgcggcg gctaccagtg ccggtgcggc gaaaacgtta gaaacgaatg cggcagcgtc
    21361 acaacaatca gcagccactt ctgcatccac cgcgaccacg aaagcgtcag aagctgccac
    21421 ctcagcccgg gatgcgtcgg cttcaaaaga ggcggcaaaa tcatcagaaa cgaacgcatc
    21481 atcaagcgcc agtagcgcag cttcctcggc gacggcggct gcaaattctg cgaaggcggc
    21541 caaaacgtcc gagacgaacg ccaggtcttc tgaaacggca gcgggacaga gtgcctcagc
    21601 tgcggcaggc tcaaaaacag cggctgcgtc gtctgccagc gccgcgtcaa caagtgccgg
    21661 gcaggcctca gccagtgcca ccgccgccgg aaaatcggca gaaagcgccg catcatccgc
    21721 ttcaacagcc acaacgaagg ctggcgaagc cactgagcag gccacggcag cagcgaggtc
    21781 agcttccgca gcgaagacat ccgaaacgaa cgcgaaagcg tcggaaaccc gtgcagaatc
    21841 ctcaaaaacg gctgccgcat cgtccgccag ttcggcggcg tcatcggcat catcggcgtc
    21901 tgcttcaaaa gatgaggcga ccagacaggc gtcagcagcg aagggcagcg ccacgacggc
    21961 atccacgaag gcgacagagg ctgctggtag tgcgacggca gcagctcaga gcaaaagtac
    22021 ggcggaatct gcagcaacgc gcgctgagac agcggcaaaa cgggcagagg atattgcatc
    22081 cgccgtggcg cttgaggatg cgagcacgac gaaaaagggg atagtacagc tcagtagtgc
    22141 gactaacagc acttccgagt cactggcggc aacaccaaaa gccgttaagg ccgcgtatga
    22201 cctggctaac gggaaataca ccgcacagga tgcaacgaca gcacagaaag ggataatcca
    22261 gctaagcagc gcgaccaaca gcacgtctga aacgctggcg gcaacgccaa aggcagtaaa
    22321 agcagccaat gacaatgctg agaaacgtct gcagaaagat cagaacggtg cggatatccc
    22381 tggcaaagac acctttacga aaaatattgg tgcctgccgt gccttcggtg ggtcagtaag
    22441 cacaacaaca ggaaactgga cgactgcaca gtttatcgag tggctggatt ctcagggagc
    22501 atttaaccat ccatactgga tgtgcaaggg ttcctggtct tatggcaata ataaaatcat
    22561 tactgatact ggctgcggta atattcatct cgccggagct gtcattgaag taatggggat
    22621 aaagtcagcg atgacgatcc gcattaccac tccgaccaca tccagcggtg gcggaacaac
    22681 caacgcccag tttacctata ttaatcacgg aacagattat tcacctggct ggcgaaggga
    22741 ctataactcc agaaataagc caacggcatc agagatcggg gcgctaccgt caggtggaac
    22801 agcagtatca tcagttaatc tggcttcaaa aggtcgggta gccgcgctga cagacaatac
    22861 gcagggggca gcaggtcttg agttatacga ggtgtataac aacggatatc caacagcgta
    22921 tggaaatatc attcacctga aagggatgac agccgttggc gaaggtgagt tactcatcgg
    22981 ctggagtggt acaagcggtg ctcatgctcc ggcatttatt cgttcacgac gggatacgac
    23041 cgacgcaaac tggtcgccgt gggcgcagct ttacacctcg gctcatcctc ctgcagagtt
    23101 ttatccagtc ggtgcaccaa tcccgtggcc atcagatacc gttccgtctg gttatgccct
    23161 gatgcagggg cagacttttg acaaatctgc ttacccgaaa cttgcagccg cttatccgtc
    23221 aggcgtgatc cctgatatgc gtggctggac gattaagggc aagcccgcca gtggtcgtgc
    23281 cgtattgtct caggaacagg atggcattaa atcgcacacc cacagcgcca gcgcatccag
    23341 tacggatttg gggacgaaaa ccacatcgtc gtttgattac ggcactaaat ccacgaataa
    23401 caccggggcg catacgcaca gtctgagcgg ctctacaagt tcagctgggg cccaccaaca
    23461 tagtcagact gggcccagaa cgaatagcgg gagtcaacct actggaatgt ttccggcagg
    23521 gagtactcaa gttagcggga ctaatcaggt tggtatcagt ggctcattaa ccagtggcac
    23581 ctcccaatgg gtaggtaaat catcttcaga aggaaaccat acacactcac tgtccggcac
    23641 agcagcatct gcgggtgcac acgcgcatac tgtcggtatt ggtgctcata cgcactctgt
    23701 tgcgattggt tcacatggac acaccatcac cgttaacgct gctggtaacg cggaaaacac
    23761 cgtcaaaaac atcgcattta actatattgt gaggctcgca taatggcatt cagaatgagt
    23821 gaacaagcac ggaccataaa aatttataac ctgctggccg gaactaatga atttattggt
    23881 gaaggtgacg catatattcc gcctcataca ggtctgcctg caaacagtac cgatattgca
    23941 ccgccagata ttccggctgg tttcgtggct gttttcaaca gtgatgagtc atcgtggcat
    24001 ctcgttgaag atcatcgggg taaaacggtt tatgacgtgg cttccggcga cgcgttattt
    24061 atttctgaac tcggctcatt accggaaaat gtcacctggt tatccccgga aggggagttt
    24121 cagaagtgga acggcacagc ctgggtgaag gatacggaag cagaaaaact gttccggatc
    24181 cgggaggcgg aagaaacaaa aaacagcctg atgcaggtag ccagtgagca tatagcgccg
    24241 cttcaggatg ctgtggatct ggagatcgca acggagaaag aaaccttgtt gctggaagct
    24301 tggaaaaagt atcgggtgtt gctgaaccgt gttgatacat caactgcacc ggatattgaa
    24361 tggccagtag cacctatagg gtaaattcgt aatgattacc taaatacgtt attcttttgt
    24421 taaaaaagtg attttgctat aggtagggag tatgttcttg gattaagaat aaaaaatcgc
    24481 catgaaagag cttcatggcg agtacataaa cgtacaaaat gtgatgaaag tagagtggcg
    24541 taagccacta tattaagata tgacaatagc gaagaaaagt aaataacatt ttatggacgt
    24601 ttaattgtta attataaaaa ctagagtgcc cgatattgtt tatcttgctt taaaataatg
    24661 tactcaaacc aaatttttga gctaaaagct caaataatat aaatgcaata ataatgttta
    24721 ataagacaat tagcttgctt tgtattttca tttcggaata ctgttaagta actttgatga
    24781 ccctaacaat agatcggaat tttcttagag tcaatggtca ctgtttttgc ttgcaaagca
    24841 aatgttttgg tggttataag tcgtcttaat atagtcacgc atgcaacttg ttccatgcgg
    24901 agattaaaga ttggaatata acgcgaagat ggaattaaat tacatttgca ataattaatt
    24961 t
//
LOCUS       DADMKF010000052        23761 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.053, whole genome shotgun sequence.
ACCESSION   DADMKF010000052 DADMKF010000000
VERSION     DADMKF010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23761
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.053"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(506..1303)
                     /locus_tag="JNC24_001915"
     CDS             complement(506..1303)
                     /locus_tag="JNC24_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY9158665.1"
                     /translation="MLAKDRTNLKIEEIRMHKHHEIHRVKPLMPASCRIRQGKKVINW
                     ETHSLTVDNNQIILFPCGYEFYIANYPEAGLYLAEMLYYPIDLIEKFQNLYAITDQIR
                     NTTGFCLPQNSELIYCWEQLKTSISRGFSTQIQEHLAMGVLLSLGAHHVNCLLLSDSK
                     QSLISRCYNLMLSEPGTKWTANKVARYLYISVSTLHRRLASEGVSFQSILDDVRLNNA
                     LSAIQTTVKPISEIARENGYKCPSRFTERFHNRFKITPRELRKASRE"
     gene            complement(1313..1864)
                     /gene="ybcL"
                     /locus_tag="JNC24_001916"
     CDS             complement(1313..1864)
                     /gene="ybcL"
                     /locus_tag="JNC24_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HAY9158666.1"
                     /translation="MKKLIVSSVLAFITFSAQAAAFQVTSNEIKTGEQLTTSHVFSGV
                     GCEGGNTSPSLTWSGAPEGTKSFAVTVYDPDAPTGSGWWHWTVANIPATVTYLPADAG
                     RRDGTKLPTGAVQGRNDFGYAGFGGACPPKGDKPHHYQFKIWALKTDKIPVDSNSSGA
                     LVGYMLNANKIATAEIIPVYEVK"
     gene            complement(2033..2365)
                     /gene="emrE"
                     /locus_tag="JNC24_001917"
     CDS             complement(2033..2365)
                     /gene="emrE"
                     /locus_tag="JNC24_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family multidrug efflux protein EmrE"
                     /protein_id="HAY9158667.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSAPH"
     gene            complement(2699..3013)
                     /gene="fliE"
                     /locus_tag="JNC24_001918"
     CDS             complement(2699..3013)
                     /gene="fliE"
                     /locus_tag="JNC24_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HAY9158668.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQESLPQPTISFAGQLHAALDR
                     ISDTQTAARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            3229..4887
                     /gene="fliF"
                     /locus_tag="JNC24_001919"
     CDS             3229..4887
                     /gene="fliF"
                     /locus_tag="JNC24_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body M-ring protein FliF"
                     /protein_id="HAY9158669.1"
                     /translation="MNATAAQTKSLEWLNRLRANPKIPLIVAGSAAVAVMVALILWAK
                     APDYRTLFSNLSDQDGGAIVSQLTQMNIPYCFSEASGAIEVPADKVHELRLRLAQQGL
                     PKGGAVGFELLDQEKFGISQFSEQVNYQRALEGELSRTIETIGPVKGARVHLAMPKPS
                     LFVREQKSPSASVTVNLLPGRALDEGQISAIVHLVSSAVAGLPPGNVTLVDQGGHLLT
                     QSNTSGRDLNDAQLKYASDVEGRIQRRIEAILSPIVGNGNIHAQVTAQLDFASKEQTE
                     EQYRPNGDESHAALRSRQLNESEQSGSGYPGGVPGALSNQPAPANNAPISTPPANQNN
                     RQQQASTTSNSGPRSTQRNETSNYEVDRTIRHTKMNVGDVQRLSVAVVVNYKTLPDGK
                     PLPLSNEQMKQIEDLTREAMGFSEKRGDSLNVVNSPFNSSDESGGELPFWQQQAFIDQ
                     LLAAGRWLLVLLVAWLLWRKAVRPQLTRRAEAMKAVQQQAQAREEVEDAVEVRLSKDE
                     QLQQRRANQRLGAEVMSQRIREMSDNDPRVVALVIRQWINNDHE"
     gene            4880..5875
                     /gene="fliG"
                     /locus_tag="JNC24_001920"
     CDS             4880..5875
                     /gene="fliG"
                     /locus_tag="JNC24_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HAY9158670.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTYVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            5868..6554
                     /gene="fliH"
                     /locus_tag="JNC24_001921"
     CDS             5868..6554
                     /gene="fliH"
                     /locus_tag="JNC24_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HAY9158671.1"
                     /translation="MSDNLPWKTWTPDDLAPPQAEFVPMVEPEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHEQGYQEGLAQGLEQGLAEAKSQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            6554..7927
                     /gene="fliI"
                     /locus_tag="JNC24_001922"
     CDS             6554..7927
                     /gene="fliI"
                     /locus_tag="JNC24_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellum-specific ATP synthase FliI"
                     /protein_id="HAY9158672.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPSPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            7946..8389
                     /gene="fliJ"
                     /locus_tag="JNC24_001923"
     CDS             7946..8389
                     /gene="fliJ"
                     /locus_tag="JNC24_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FliJ"
                     /protein_id="HAY9158673.1"
                     /translation="MAEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLID
                     YQNEYRNNLNSDMSAGMTSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWR
                     EKKQRLQAWQTLQERQSTAALLAENRLDQKKMDEFAQRAAMRKPE"
     gene            8386..9513
                     /gene="fliK"
                     /locus_tag="JNC24_001924"
     CDS             8386..9513
                     /gene="fliK"
                     /locus_tag="JNC24_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook length control protein FliK"
                     /protein_id="HAY9158674.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLALLSEALASETTTD
                     KAAPQVLVATDKPTTKGELLISDIVSDAQQADLLIPVDETLPVINVEQSTSTPLTTAH
                     TMTLAAVADKNTTKDEKADDLNEDLTASLSALFAMLPGFDNTPKVTDAPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTAAASPLITPH
                     QTQPLLTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMVSPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTVNHEPLAGEEDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            9618..10082
                     /gene="fliL"
                     /locus_tag="JNC24_001925"
     CDS             9618..10082
                     /gene="fliL"
                     /locus_tag="JNC24_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HAY9158675.1"
                     /translation="MTDYAISKKSKRSLWLPILVFITLAACASAGYSYWHSHQVAAED
                     KAQHRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVMYTAFILR"
     gene            10087..11091
                     /gene="fliM"
                     /locus_tag="JNC24_001926"
     CDS             10087..11091
                     /gene="fliM"
                     /locus_tag="JNC24_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HAY9158676.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGNGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDGIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            11088..11501
                     /gene="fliN"
                     /locus_tag="JNC24_001927"
     CDS             11088..11501
                     /gene="fliN"
                     /locus_tag="JNC24_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HAY9158677.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSSKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLISQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            11504..11869
                     /gene="fliO"
                     /locus_tag="JNC24_001928"
     CDS             11504..11869
                     /gene="fliO"
                     /locus_tag="JNC24_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliO"
                     /protein_id="HAY9158678.1"
                     /translation="MNNHATVQSSTPVSAAPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRSGRS"
     gene            11869..12606
                     /gene="fliP"
                     /locus_tag="JNC24_001929"
     CDS             11869..12606
                     /gene="fliP"
                     /locus_tag="JNC24_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HAY9158679.1"
                     /translation="MRRLLSVAPVLLWLVTPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     SVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            12616..12885
                     /gene="fliQ"
                     /locus_tag="JNC24_001930"
     CDS             12616..12885
                     /gene="fliQ"
                     /locus_tag="JNC24_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HAY9158680.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            12894..13679
                     /gene="fliR"
                     /locus_tag="JNC24_001931"
     CDS             12894..13679
                     /gene="fliR"
                     /locus_tag="JNC24_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliR"
                     /protein_id="HAY9158681.1"
                     /translation="MMQVTSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFVIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPASHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     GEPLNSNAFLALTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEMFNLLADIISELPLI"
     gene            13964..14587
                     /gene="rcsA"
                     /locus_tag="JNC24_001932"
     CDS             13964..14587
                     /gene="rcsA"
                     /locus_tag="JNC24_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HAY9158682.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQHIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKKLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(14631..14873)
                     /gene="dsrB"
                     /locus_tag="JNC24_001933"
     CDS             complement(14631..14873)
                     /gene="dsrB"
                     /locus_tag="JNC24_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HAY9158683.1"
                     /translation="MPEKRNCSTLAQSHRRKTMKVNDRVTVKTDGGPRRPGVVLAVEE
                     FSEGTMYLVSLEDYPLGIWFFNEAGHQDGIFVEKAE"
     gene            14982..15209
                     /gene="yodD"
                     /locus_tag="JNC24_001934"
     CDS             14982..15209
                     /gene="yodD"
                     /locus_tag="JNC24_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HAY9158684.1"
                     /translation="MKTAKEYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            15507..16322
                     /locus_tag="JNC24_001935"
     CDS             15507..16322
                     /locus_tag="JNC24_001935"
                     /EC_number="3.1.3.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
                     /protein_id="HAY9158685.1"
                     /translation="MFSIQQPLLVFSDLDGTLLDSHSYDWQPTALWLSRLREANVPVI
                     LCSSKTSAEMLYLQKTLGLQGLPLIAENGAVIQLAEQWQDIDGFPRIISGISHGEISQ
                     VLNTLREKEHFKFTTFDDVDDATIAEWTGLSRSQAALTQLHEASVTLIWRDSDERMAQ
                     FTARLNELGLQFMQGARFWHVLDASAGKDQAANWIIATYQQLSGKRPTTLGLGDGPND
                     APLLEVMDYAVIVKGLNREGVHLHDEDPTRVWRTQREGPEGWREGLDHFFSAR"
     gene            complement(16319..18013)
                     /gene="yedQ"
                     /locus_tag="JNC24_001936"
     CDS             complement(16319..18013)
                     /gene="yedQ"
                     /locus_tag="JNC24_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator YedQ"
                     /protein_id="HAY9158686.1"
                     /translation="MQHETKMENQSWLKKLARRLGPGHVVNLCFIVVLLFSTLLTWRE
                     VVVLEDAYISSQRNHLENVANALDKHLQYNVDKLIFLRNGMREALVAPLDFTSLRNAV
                     TEFEQHRDEHAWQIELNRRRTLSVNGVSDALVSEGNLLSRENESLDNEITAALEVGYL
                     LRLAHNTSSMVEQAMYVSRAGFYVSTQPTLFTRNVPTRYYGYVTQPWFIGHSQRENRH
                     RAVRWFTSQPEHASNTEPQVTVSVPVDSNNYWYGVLGMSIPVRTMQQFLRNAIDKNLD
                     GEYQLYDSKLRFLTSSNPDHPTGNIFDPRELALLAQAMEHDTRGGIRMDSRYVSWERL
                     DHFDGVLVRVHTLSEGVRGDFGSISIALTLLWALFTTMLLISWYVIRRMVSNMYVLQS
                     SLQWQAWHDTLTRLYNRGALFEKARPLAKLCQTHQHPFSVIQVDLDHFKAINDRFGHQ
                     AGDRVLSHAAGLISSSLRAQDVAGRVGGEEFCVILPGASLTEAAEVAERIRLKLNEKE
                     MLIAKSTTIRISASLGVSSSEETGDYDFEQLQSLADRRLYLAKQAGRNRVCASDNA"
     gene            complement(18184..18366)
                     /locus_tag="JNC24_001937"
     CDS             complement(18184..18366)
                     /locus_tag="JNC24_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HAY9158687.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETELVPGEGSRSAEEV"
     gene            complement(18445..19362)
                     /locus_tag="JNC24_001938"
     CDS             complement(18445..19362)
                     /locus_tag="JNC24_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HAY9158688.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHFAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            19535..20456
                     /gene="yedA"
                     /locus_tag="JNC24_001939"
                     /pseudo
     CDS             19535..20456
                     /gene="yedA"
                     /locus_tag="JNC24_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310724.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
     gene            complement(20445..20915)
                     /locus_tag="JNC24_001940"
     CDS             complement(20445..20915)
                     /locus_tag="JNC24_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="very short patch repair endonuclease"
                     /protein_id="HAY9158689.1"
                     /translation="MADVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGSPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRGREKLTDEALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(20896..22314)
                     /locus_tag="JNC24_001941"
     CDS             complement(20896..22314)
                     /locus_tag="JNC24_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HAY9158690.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKTLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPSAHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHKEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDQGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            complement(22381..23037)
                     /locus_tag="JNC24_001942"
     CDS             complement(22381..23037)
                     /locus_tag="JNC24_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HAY9158691.1"
                     /translation="MDLQHWQAQFENWLKNHHQHQDAAQKLAADDDVDMLVILTACYF
                     HDIVSLAKNHPQRQRSSILAAEETRRLLREEFEQFPAEKIEAVCHAIAAHSFSAQIAP
                     LTTEAKIVQDADRLEALGAIGLARVFAVSGALGVALFDGEDPFAQHRPLDDKRYALDH
                     FQTKLLKLPQTMQTARGKQLAQHNAHFLVEFMAKLSAELAGENEGVDHKVIDAFSPAG
                     "
     gene            complement(23077..23442)
                     /locus_tag="JNC24_001943"
     CDS             complement(23077..23442)
                     /locus_tag="JNC24_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HAY9158692.1"
                     /translation="MEKCDFYHIIVLSLNFPGYLKMEYGSTKMEERLSRSPGGKLALW
                     AFYTWCGYFVWAMARYIWVMSRIPDAPVSGFESDLGSTAGKWLGALVGFLFMALVGAL
                     LGSIAWYTRPRPARSRRYE"
BASE COUNT         5911 a         6157 c         5907 g         5786 t
ORIGIN      
        1 atctacttca caatttaaat gagaaactaa aacttacatc ttgaaataat ctcattgatt
       61 ggatgaatat ttgtagtgca gtgacatcat ttttaaataa tagttcaaat aaaagggctc
      121 acgatgaaaa aattaacagt ggcaatttct gctgtagctg catcttaacg tataaactag
      181 atttaataat aacgaactag atttaataac aacgatatcc gtacctatcc ggatatctaa
      241 tgtcataagc atccctttaa ttatcccctc aacctcttgt aactttccca ctcttgaact
      301 ccacatagtc caaacgccgg atgcaaaaaa atcacacgtg catttctgaa attgtgatgg
      361 aggaagatag tatcaattat atgtgcttat aaacgtcccc gattagcgca gaagtcaccg
      421 gattgttcag gctccaatag gttgagtatg ccgtatttat tttccaaaat aaaaatcgat
      481 gctgttgctc cttcataaaa tacttttact ctctggacgc ttttcttagc tctcttggtg
      541 ttatcttaaa acgattatgg aacctttcag taaaacgaga aggacacttg taaccatttt
      601 ccctggcaat ctcgctgatg ggttttactg tcgtttgtat agcagacaac gcattattta
      661 acctcacgtc gtccaatata ctttgaaaac ttactccctc gcttgccaga cggcgatgca
      721 atgtggaaac agaaatgtag agatatctcg ctaccttgtt tgctgtccat tttgttccag
      781 gttcggatag cattaggtta taacaacgac ttattaatga ttgtttacta tctgataaaa
      841 gtaaacaatt aacatgatgt gctcctaatg aaagtagaac gcccattgct aagtgttcct
      901 gaatttgcgt tgagaagcct cgggaaatag atgtttttag ttgctcccaa caatatatta
      961 actcggaatt ctgaggtaag cagaaacctg tcgtgttacg aatttgatcg gttatcgcat
     1021 aaagattttg gaacttttca attagatcaa tgggataata aagcatttct gcaagataaa
     1081 gacctgcttc tgggtaattc gcaatataaa attcatatcc acagggaaat aatattatct
     1141 gattattatc aacagttaga ctatgcgtct cccaattgat aacttttttt ccctgacgga
     1201 tacgacatga agctggcata agaggcttaa ccctgtgaat ctcatgatgt ttatgcatcc
     1261 gtatttcttc aatctttagg ttagtcctat cttttgccag catactctta tcctacttta
     1321 cctcataaac gggtattatc tcagcggttg caattttatt ggcattaagc atataaccaa
     1381 ctaacgctcc gctggaatta gaatccacag gaatcttatc agtttttaga gcccatattt
     1441 taaactggta atgatggggt ttatctcctt tgggaggaca tgctccacca aatcccgcat
     1501 agccaaaatc atttcggcct tggacagcac cagtcggaag ttttgtacca tcacgtcttc
     1561 ctgcatcagc gggcaaatat gttaccgttg ctggaatatt agcaacagtc cagtgccacc
     1621 atccgctgcc tgtaggcgca tctggatcat acacagttac ggcaaagctt ttggtgcctt
     1681 ctggagcacc agaccaggtt aatgagggcg atgtattacc cccctcacac ccaactccag
     1741 aaaatacatg agacgttgta agttgctctc ctgtttttat ttcattacta gtgacctgaa
     1801 atgctgcggc ctgcgcagaa aatgttatga atgccaatac acttgaaacg ataagttttt
     1861 tcataaaaac ctctttgttg tgacctatcg ttattttatt tgatgtgcat tgatctcatt
     1921 atgcaaaaag gcgcaatgat cgcgcaaatg caatcataat tataaagttg ccccgaacat
     1981 tagccataat atgcacaaat cagattcctg ttatctcaag gatgattatt ttttaatgcg
     2041 gtgcgcttcg tgacaacaag ttaatgacta acacaccggc acaaatcaac atcatgccta
     2101 tgatggctgg caggtccagt cgttggccaa aaaatcccca ggaaagcaaa ctaatcagaa
     2161 cgataccgac tcctgaccag atagcataag caatccctgt aggtatataa gccagtgtct
     2221 gagctaataa ccaaaatgat gcacaatagc aaataattgt gccaacagag ggccataacc
     2281 gtgtaaaccc ctctgaaaac ttcattaagg ttgtaccaat gacctctgca agtattgcgc
     2341 caccaagata aatataaggg ttcatagcat attctttcct gttcatactg gagagaattg
     2401 tactacagtt tgaacacaac tcaccgtttt catcctggtg tatgtgtatc cagggggctc
     2461 tggcgtggca agagataaag gcaggaacaa taatgatcag aaggaacccc aggctaacaa
     2521 aagagttcac tcttcctatg agcaaagcga agacaaaaca tgttatcgcg tttacggatc
     2581 gtggatggca gaaaactttc aggatcaggt atccatttta aaccagaaat tgagtgagtt
     2641 tgccccatcc atgccccacg cggtgagatc ggatgtaatt aacaacaggt tacaaaacct
     2701 acacctgcat ggacatcact tcctgataag ccgccaccag cttattacgc acctgaatcc
     2761 ccatttgcat agaaactgag gctttttgca tatcggtcat cacatcgttt aacgccacgc
     2821 cgggttcacc gagagtgaat ttttctgcct gcgtgcgggc ggctgtttgt gtatcgctta
     2881 tgcgatcgag cgcggcgtgc agctgcccgg caaaactaat ggtcggttgt ggcagtgatt
     2941 cctgcgcacg cgcactcatc gccgtagcct gtaactggct gataacccct tcaatcccct
     3001 gtatcgctga cattttcatc tcccggataa tttctggtag caaagcttac cagtaagtca
     3061 ataagacaaa ggcgctaaat agcaacaaaa aaaacgggtt tattggcgga tagaaaaaaa
     3121 cgaaagcaca aataatggca gcgtcaattt ttcgagtttg ctgacccggg agtgagtctt
     3181 gttccacttt gccaataacg ccgtccataa tcagccacga ggtgcgcgat gaatgcgact
     3241 gcagcccaga caaaatctct tgagtggctt aatcgcctgc gtgcgaatcc gaaaattcca
     3301 ttgattgttg ccggttccgc ggcagtggcg gtcatggtcg cactgatcct gtgggcgaaa
     3361 gcccccgact accgcacatt attcagcaat ctttccgatc aggatggtgg cgcaattgtc
     3421 agccaactga cgcaaatgaa tattccttac tgcttcagcg aagccagcgg cgctattgaa
     3481 gttccggcag ataaagttca cgaactgcgt ctgcgcctgg cacaacaagg tttgcccaaa
     3541 ggcggcgcgg tcggtttcga actgcttgat caggaaaagt ttggtatcag ccagttcagc
     3601 gaacaggtga attatcagcg ggcgctggaa ggcgagcttt ctcgtaccat cgaaactatc
     3661 ggcccggtaa aaggggcgcg cgtacatctg gcaatgccga aaccgtcttt attcgtccgt
     3721 gaacaaaaat ccccttctgc atcggtgacg gtaaatctgt tacccggccg cgcactcgat
     3781 gaagggcaaa ttagcgccat tgtgcacctg gtttccagcg ccgttgctgg tctgccgccg
     3841 ggaaacgtca cgctggtgga tcagggcgga catctgttaa cccagtccaa taccagcggg
     3901 cgcgatctta atgacgctca gttgaaatat gccagcgatg tcgaaggccg tattcagcgg
     3961 cgtattgaag caatcctgtc gcctattgtt ggtaacggta atattcacgc ccaggtcacg
     4021 gcgcaactgg acttcgccag taaagaacaa acggaagaac agtatcgccc taacggtgat
     4081 gaatctcatg cggcgcttcg ttcacgccag cttaatgaga gcgagcaaag cggttccggt
     4141 tatccgggcg gcgtaccggg ggcgttgtcg aatcaaccgg cacctgcgaa taacgcgcca
     4201 atcagcacgc ctccggcaaa tcaaaataac cgccagcagc aggcgagcac caccagcaat
     4261 agcgggccgc gtagcacaca gcggaatgaa accagtaact acgaagtcga tcgcaccatt
     4321 cgtcatacca aaatgaacgt gggcgatgtg caacgtctgt cagtcgcggt agtggtgaat
     4381 tacaaaacct tgccagacgg taaaccattg cctctcagca acgaacagat gaagcaaatt
     4441 gaagatctga cccgcgaagc gatgggcttt tctgaaaaac gcggtgactc gctcaatgtc
     4501 gttaactcgc cgttcaatag cagtgacgaa agcggcggag aactgccgtt ctggcaacag
     4561 caagcgttta tcgatcagtt gctggctgcc ggtcgctggt tgctggtact gctggtggcg
     4621 tggctgctgt ggcggaaagc ggtacgcccg cagttaacac gtcgcgccga ggcgatgaaa
     4681 gctgtacagc aacaggcgca ggcccgtgag gaagtggaag atgcggtgga agtccgcctg
     4741 agcaaagacg aacaactcca acaacgacgc gctaaccaac gtctgggggc agaagtcatg
     4801 agccagcgta tccgtgaaat gtctgataac gatccgcgcg tggtggcgct ggtcattcgc
     4861 cagtggataa ataacgatca tgagtaacct gacaggcacc gataaaagcg tcatcctgct
     4921 gatgaccatt ggcgaagacc gggcggcaga ggtgttcaag cacctctccc agcgcgaagt
     4981 gcaaaccctg agcgctgcaa tggcgaacgt cacgcagatc tccaacaagc agctaaccta
     5041 tgtgctggcg gagtttgagc aagaagctga acagtttgcc gcactgaata tcaacgccaa
     5101 cgattatctg cgttcggtat tggtcaaagc tctgggtgag gaacgtgccg ccagcctgct
     5161 ggaagatatt ctcgaaactc gcgataccgc cagcggtatt gaaacgctca actttatgga
     5221 gccgcagagt gccgccgatc tgattcgcga tgagcatccg caaattatcg ccaccattct
     5281 ggtgcatctg aagcgcgcgc aggccgccga tattctggcg ttgttcgatg aacgtctgcg
     5341 ccacgacgtg atgttgcgta tcgccacctt tggcggcgtg cagccagccg cgctggcgga
     5401 actgaccgaa gtactgaatg gcttgctcga cggtcagaat ctcaagcgca gcaaaatggg
     5461 cggcgtgaga acggcagccg aaattatcaa cctgatgaaa actcagcagg aagaagccgt
     5521 tattaccgcc gtgcgcgaat tcgacggcga gctggcgcaa aaaattatcg acgagatgtt
     5581 cctgttcgaa aatctggtgg atgtcgacga tcgcagcatt cagcgtctgt tgcaggaagt
     5641 ggattccgaa tcgctgttga tcgcgctgaa aggggccgag cagccactgc gcgagaaatt
     5701 cctgcgcaat atgtcgcagc gtgccgccga tattctgcgc gacgatctcg ccaaccgtgg
     5761 tccggtgcgt ctgtcgcagg tggaaaacga acagaaagcg attctgctga ttgtgcgccg
     5821 ccttgccgaa actggcgaga tggtaattgg cagcggcgag gatacctatg tctgataatc
     5881 tgccgtggaa aacctggacg ccggacgatc tcgcgccacc acaggcagag tttgtgccca
     5941 tggtcgagcc ggaagaaacc atcattgaag aggccgaacc tagccttgag cagcaactgg
     6001 cgcaactgca aatgcaggcc catgagcaag gttatcaggc ggggattgcc gaaggtcgcc
     6061 agcaaggtca tgagcagggc tatcaggaag gactggccca ggggctggag caaggtctgg
     6121 cagaggcgaa gtctcaacaa gcgccaattc atgcccggat gcagcaactg gtcagcgaat
     6181 ttcaaactac ccttgatgcg cttgatagtg tgattgcttc gcgcctgatg cagatggcgc
     6241 tggaggcggc acgtcaggtg atcggtcaga cgccaacagt ggataactcg gcactgatca
     6301 aacagatcca acagttgttg cagcaagaac cgttattcag cggtaaacca cagctgcgcg
     6361 tgcacccgga tgatctgcaa cgtgtggatg atatgctcgg cgcaacctta agtttgcatg
     6421 gctggcgctt gcggggcgat cccacactcc atcctggcgg ctgtaaagtc tccgccgatg
     6481 aaggcgatct cgacgccagt gtcgccactc gctggcaaga actctgccgt ctggcagcac
     6541 caggagtggt gtaatgacca cgcgcctgac tcgctggcta accacgctgg ataactttga
     6601 agccaaaatg gcgcagttgc ctgcggtacg tcgctatggg cgattaaccc gcgctaccgg
     6661 gctggtgctg gaagccaccg gattacaatt gccgctcggc gcaacctgtg tcattgaacg
     6721 ccagaacggc agcgaaacgc acgaagtaga aagcgaagtc gttggcttta acggtcaacg
     6781 gctgttttta atgccgctgg aggaagtgga aggtgtcctg cccggcgcgc gggtttatgc
     6841 caaaaacatt tcggctgaag gactgcaaag cggcaagcag ttgccgctcg gtccggcgtt
     6901 attaggtcgc gttctggacg gcagcggtaa gccgctcgat ggcctgccct cccccgatac
     6961 gacggaaacc ggtgcgctga ttaccccgcc atttaacccg ttgcaacgta caccgattga
     7021 acatgtgctg gacaccggcg tgcgcccaat caatgccctg cttaccgttg ggcgtgggca
     7081 gcgtatgggg ctgtttgccg ggtccggcgt tggtaaaagt gtgctgctgg ggatgatggc
     7141 acgttacacc cgcgccgatg tcattgtcgt gggtttgatt ggtgaacgtg ggcgcgaagt
     7201 aaaagatttt attgagaata tcctcggtgc tgaagggcgt gcacgctccg tggtgattgc
     7261 cgctccggcg gatgtctctc cgcttctgcg aatgcaaggt gccgcctatg ccacgcgcat
     7321 tgccgaagat tttcgcgatc gtggtcagca tgtgttgctg attatggact ccctcacccg
     7381 ctacgcgatg gcccagcgtg aaattgcgct ggcgattggc gaaccacccg ccaccaaagg
     7441 ttatccaccg tcggtgtttg ccaaattacc ggcactggtc gagcgtgccg gaaatggcat
     7501 tagcggcggc ggctcgatta ccgcgtttta taccgtgctt actgaaggcg atgaccagca
     7561 ggaccccatt gcagactccg cacgggcgat cctcgacggt cacattgtgc tgtctcgccg
     7621 actggcggaa gccgggcact atccggctat cgatattgaa gcgtcgatca gccgcgcaat
     7681 gacggcgttg atcagtgagc aacattacgc gcgagtgcgc accttcaaac agctgttgtc
     7741 gagttttcag cgtaaccgcg atctggttag cgtcggcgcg tatgccaaag gcagcgatcc
     7801 gatgctcgat aaagccatcg ccctgtggcc gcagctggag ggctatttac aacaaggcat
     7861 ttttgaacgt gcggactggg aagcgtctct ccaggggctg gagcgtattt tcccgacagt
     7921 gtcataaccc aggagataac ggtagatggc agaacatggt gcgctggcga ccctgaaaga
     7981 tctggcagaa aaagaggtag aggatgccgc gcgcctgctg ggtgaaatgc gtcgcgggtg
     8041 tcagcaggcg gaagaacagc tcaaaatgct gatcgattat cagaatgaat atcgcaataa
     8101 cctcaacagc gatatgagtg ccgggatgac cagcaaccgc tggatcaact atcagcagtt
     8161 tatccagacg ctggaaaaag ccattactca gcatcgccag caacttaatc agtggacgca
     8221 gaaagttgac attgccctga acagctggcg agaaaagaaa caacgtttgc aggcctggca
     8281 gacactgcag gaacggcaat ccacggcggc actgcttgca gaaaaccgcc tcgatcagaa
     8341 aaagatggat gagttcgccc agcgcgccgc catgaggaaa cctgaatgat tcgcttagcg
     8401 cccttgatta ccgccgacgt tgacaccacc acattgcctg gcggcaaagc cagcgatgct
     8461 gcacaagatt ttctcgcgtt gttgagcgaa gcattagcaa gcgagacaac taccgacaaa
     8521 gcggcccccc aggtgctggt ggcaacagat aagcccacga caaaaggcga gctgctgatc
     8581 agcgatattg tttccgacgc gcaacaagcg gatttactga tccctgtgga tgaaacactg
     8641 cctgtcatca acgtcgaaca atccacatcg acaccgttaa ccaccgctca tacgatgacg
     8701 ttggctgcgg tggctgacaa aaatacgaca aaagacgaaa aagcggatga tctgaatgaa
     8761 gacctcaccg caagcctgag cgcccttttt gcgatgttgc cgggttttga caatacgccc
     8821 aaagtgactg atgcgccgtc aaccgtgtta ccggcagaga aaccaacgct cttcacaaaa
     8881 ctgacttctg cgcaactcac aacagcacag cctgatgatg cccccggcac gccagctcag
     8941 ccattaacac cgctggtagc agaagcccag agtaaagcgg aagtcatcag cacaccttca
     9001 ccggtgaccg ctgccgccag cccgctaatc actccacacc agacacagcc actgctcacc
     9061 gtcgccgcgc ctgtcttgag tgcaccgctg ggttctcacg aatggcaaca atcattaagc
     9121 cagcatattt cgctgttcac ccgccaggga caacaaagtg cagagttgcg tctgcacccg
     9181 caggatttag gtgaagtgca aatctccctc aaagtggatg ataaccaggc gcaaatccag
     9241 atggtttcgc cgcatcagca cgtacgcgcc gccctggaag cagcgctgcc ggtactgcgt
     9301 acgcagctgg ccgagagtgg cattcagtta gggcaaagca acatcagtgg cgaaagcttt
     9361 agtggtcagc agcaggccgc ttcccaacaa cagcaaagcc aacgcacagt aaaccatgaa
     9421 cctctggcgg gggaagaaga cgatacgctt ccggttcccg tctctttaca agggcgcgta
     9481 acaggcaaca gcggcgttga tattttcgcc taacgtcaga ggtagcaccg taatccgcgt
     9541 cttttccccg ctttgttgcg ctcaagacgc aggataatta gccgataagc agtagcgaca
     9601 caggaagacc gcaacacatg actgattacg cgataagcaa gaaaagcaag cgatcgcttt
     9661 ggctcccgat tctggtattc attaccctcg cggcctgtgc cagcgcaggt tacagctact
     9721 ggcattcgca tcaggttgcc gctgaagaca aagcgcagca tcgcgtcgtg ccctcaccgg
     9781 tcttctatgc gctggatacc tttacggtca atttgggcga tgcggatcgc gtgctttata
     9841 tcggtataac cctgcgactc aaagatgaag ctacccgctc gcggctgagt gagtatttgc
     9901 cggaagtccg tagccgtttg ctgttactgt tttcgcgtca ggatgctgcc gtactggcga
     9961 cagaagaagg caagaaaaac ctgattgccg agattaaaac cacgctttcc accccgcttg
    10021 ttgccgggca accgaaacag gatgtcaccg acgtgatgta taccgctttt attctgcgat
    10081 aacgacatgg gcgatagtat tctttctcaa gctgaaattg atgcgctgtt gaatggtgac
    10141 agcgaagtca aagacgaacc gacagccagt gttagcggcg aaagtgacat tcgtccgtac
    10201 gatccgaata cccaacgacg ggttgtgcgc gaacgtttgc aggcgctgga aatcattaat
    10261 gagcgctttg cccgccattt tcgtatgggg ctgttcaacc tgctgcgtcg tagcccggat
    10321 ataaccgtcg gggccatccg cattcagccg taccatgaat ttgcccgcaa cctgccggtg
    10381 ccgaccaacc tgaaccttat ccatctgaaa ccgctgcgcg gcacagggct ggtggtgttt
    10441 tcaccgagtc tggtgtttat cgccgtggat aacctgtttg gcggcaatgg acgcttcccg
    10501 accaaagtgg aaggtcgcga gtttacccat accgaacagc gcgtcatcaa ccgcatgtta
    10561 aagctggcgc ttgaaggcta tagcgacgcc tggaaggcga ttaatccgct ggaagttgag
    10621 tacgtgcgtt cggaaatgca ggtgaaattt accaatatca ccacctcgcc gaacgacatt
    10681 gtggttaaca cgccgttcca tgtggagatt ggcaacctga ccggcgaatt taatatctgt
    10741 ctgccattca gcatgatcga gccgctacgg gaattgttgg ttaacccgcc gctggaaaac
    10801 tcgcgtaatg aagatcagaa ctggcgcgat aacctggtgc gccaggtgca gcattcacag
    10861 ctggagctgg tcgccaactt tgccgatatc tcgctacgcc tgtcgcagat tttaaaactg
    10921 aaacccggcg acgtcctgcc gatagaaaaa cccgatggca tcatcgccca tgttgacggc
    10981 gtcccggtgc tgaccagtca gtacggcact ctcaacggtc agtatgcgtt acggatagaa
    11041 catttgatta acccgatttt aaattctctg aacgaggaac agcccaaatg agtgacatga
    11101 ataatccggc cgatgacaac aacggcgcaa tggacgatct gtgggctgaa gcgttgagcg
    11161 aacaaaaatc aaccagcagc aaaagcgctg ccgacgcggt gttccagcaa tttggcggtg
    11221 gtgatgtcag cggaacgttg caggatatcg acctgataat ggatattccg gtcaagctga
    11281 ccgtcgagct gggccgtacg cggatgacca tcaaagagct gttgcgtctg acgcaagggt
    11341 ccgtcgtggc gctggacggt ctggcgggcg aaccactgga tattctgatc aacggttatt
    11401 taatctccca gggcgaagtg gtggtcgttg ccgataaata tggcgtgcgg atcaccgata
    11461 tcattactcc gtctgagcga atgcgtcgcc tgagccgtta gtgatgaata accacgctac
    11521 tgtgcaatct tccacgccgg tttctgctgc gccactgctg caggtcagcg gcgcactcat
    11581 cgccattatt gccctgatcc tcgctgctgc ctggctggta aaacggttgg gatttgcccc
    11641 gaaacgcact ggcgttaacg gtctgaaaat tagcgccagt gcttcgctgg gcgcgcgtga
    11701 acgggttgtg gtggtcgatg tggaagatgc acggctggtg ctcggcgtta ccgcagggca
    11761 aatcaatctg ctgcataaac ttcccccttc agcaccaaca gaagagatac cgcagaccga
    11821 ttttcagtcg gtcatgaaaa atttgcttaa gcgtagtggg agatcctgat gcgtcgtttg
    11881 ttgtctgtcg cacctgtcct tctctggctg gttacgcccc tcgccttcgc gcaactgccg
    11941 ggtatcacca gccagccgct gcctggcggt ggacaaagct ggtcgctccc ggtgcagacg
    12001 ctggtgttca tcacctcgtt gacgtttatt ccggcaattt tactgatgat gaccagtttc
    12061 acccgcatca tcattgtttt tggtttattg cgtaacgcgc tgggaacgcc ctccgcgcca
    12121 cctaaccagg tattgctggg gctggcacta tttttgacct tttttattat gtcatcggtg
    12181 atcgacaaaa tttatgtcga tgcgtaccag ccattcagcg aagagaaaat atcaatgcag
    12241 gaggcgctgg aaaaaggggc gcagccgcta cgtgagttta tgctgcgtca gacccgtgag
    12301 gcagatttag ggttgtttgc cagactagcg aataccggcc cgttgcaggg acctgaagcc
    12361 gtgccgatgc gcattttgct cccggcctac gtgaccagcg aattgaaaac cgcatttcag
    12421 ataggcttca cgattttcat tccttttttg attatcgacc tggtgattgc cagcgtgttg
    12481 atggcattgg ggatgatgat ggtccccccc gccaccattg ctctgccctt taaactgatg
    12541 ctgtttgtac tggtggatgg ctggcaattg ctggtcggtt cgctggcgca gagcttttac
    12601 agctagagag gcaaaatgac acctgaatcg gtcatgatga tggggactga agcgatgaaa
    12661 gtcgcgctgg cactggctgc cccgctgttg ctggtggcgc tggtcacggg ccttatcatc
    12721 agtattttgc aggccgccac gcagatcaac gaaatgacgc tgtcgtttat tccaaaaatc
    12781 atcgccgtat ttattgccat tattattgcc ggaccgtgga tgctcaatct gttgctggat
    12841 tacgtccgca ccttgttcac taacctgccg tatatcatcg ggtaggccgt acaatgatgc
    12901 aggtgacaag cgatcaatgg ctttcctggt taagcctgta cttctggccg ttactgcgcg
    12961 tgctggcgct catttctacc gcaccaattc tgagcgaacg cagcgtgccg aaacgggtaa
    13021 aactggggct ggcaatgatg atcacgttcg tcattgcccc atcattacct gccaacgatg
    13081 ttcctgtttt ttcgttcttt gctctgtggc tggccgtgca gcagatcctg atcggcattg
    13141 cgcttggttt taccatgcaa tttgcctttg ccgctgtgcg aaccgctggc gaaattatcg
    13201 gtctgcaaat ggggctgtca tttgcgacgt ttgtcgatcc ggccagccat ctcaatatgc
    13261 ccgttttagc gcgtatcatg gatatgctgg cgttactgct gttcctgaca tttaacggtc
    13321 atttatggtt gatttcactg ctggtcgata cctttcacac cctgccaatt ggtggcgaac
    13381 cgttgaacag caatgcgttt ctggcactca ccaaagcggg gagtttgatt ttccttaacg
    13441 ggctgatgct ggcgttaccg ctcattactc tgctgctgac cctgaatctg gcattaggtt
    13501 tactgaatcg tatggccccg caattatcca tttttgttat tggatttcca ttaactctga
    13561 ctgtcggtat ctctttaatg gcggcattaa tgccgttaat tgcacctttt tgcgaacatt
    13621 tattcagtga gatgtttaat ttgctggctg atattattag tgaattgcca ttaatataat
    13681 tccgtaacgt ttatcatgtt atcctaagga ttatccgaaa aataatacct acgaacatct
    13741 tccaggatac tcctgcggcg aaatatttgt tttaagctca ctcacatatc gcaacattta
    13801 ctttaagaca attccaggca aattatacaa cactttacgg gatagtaagt ccgcctgaaa
    13861 aatcgcgaga gtggcgcatt aggtgaccca tgttgttccg tttagtcatg atgaaatatt
    13921 caggtaaggg gaattatcgt tacgcattga gtgagggtat gccatgtcaa cgattattat
    13981 ggatttatgt agttacaccc gactaggttt aaccgggtat ctgttgagta gaggggttaa
    14041 aaaaagagaa atcaacgaca ttgaaaccgt tgatgacctt gccatagctt gtgattcaca
    14101 gcgcccttca gtggtgttta ttaatgagga ctgtttcatc catgatgctt ctaacagtca
    14161 gcacatcaag cacatcatta atcaacatcc caatacgtta tttatcgttt ttatggcaat
    14221 tgccaatgtt cattttgatg aatatctatt ggtcagaaaa aaattattga taagttctaa
    14281 atcgattaaa ccggaatctc tcgacgatat ccttggcgat attctgaaaa aagagacaac
    14341 gataacctct tttttaaata tgccgacgtt atcattgagc cgaaccgaat cgagtatgtt
    14401 gcgaatgtgg atggcaggtc agggaaccat tcaaatctct gaccaaatga atatcaaagc
    14461 caagaccgtt tcatcgcata aaggtaatat taaacgtaag atcaaaacac ataataaaca
    14521 ggttatctac catgtcgtcc gactgacgga taatgtgact aatggtattt ttgtcaacat
    14581 gcgctaacac attctgactg gtggtttccc accagtcagg ctgaataaga ttactctgct
    14641 ttctccacaa agataccgtc ctgatgccct gcttcattaa agaaccagat gccgagcggg
    14701 tagtcttcca gcgaaaccag gtacattgtg ccttcactaa actcctcaac tgccagtacc
    14761 acgccaggac gacgcggacc gccatccgtt ttgactgtta cccgatcatt caccttcatc
    14821 gttttcctcc tgtggctttg tgccagtgta gaacaatttc gtttttctgg cagctccagg
    14881 cgcgcgcgag tgctgatttt ctcgacggtc tatacttaag agatgccagg cggacttaac
    14941 gactggcggc aacaacagag taacggttgc gaggaaagat gatgaaaacc gcaaaagagt
    15001 acagcgatac cgcaaaacgt gaggtcagcg tcgatgtcga tgccctgctg gcggcgatca
    15061 atgaaattag cgaaagcgaa gttcatcgca gccagaacga ttctgaacac gttagcgtcg
    15121 atggacgtga atatcataca tggcgtgaat tggcggatgc cttcgaactg gatattcatg
    15181 acttcagcgt ctctgaagtg aatcgttgaa tgcacaataa aaaaatcccg accctgaggg
    15241 ggtcgggatg aaacttgctt aagcaagaag cacttaaaaa attcgttaca ccaggaaatc
    15301 tgatgtgttc atcaccttat ccgcaatttt tttcgctgac aagaaaatat tcgcgatatg
    15361 aataattaac gctacttatg aatagaaatg tgacgtgcgt caccccttgg tggctctggc
    15421 gtcaacggga tcgctcccga aaaaatcatc tttgattatt tgtgctgttt tgagaatcat
    15481 cctggtaagg ggagaactgc acgaccatgt tttcaattca acaaccacta ctggttttta
    15541 gcgatcttga tggcaccctg ctggacagtc atagttatga ctggcaaccg actgccctct
    15601 ggctcagccg tttacgcgaa gcgaatgttc ccgtcattct ctgtagcagt aaaacttcag
    15661 cggaaatgct gtacttgcaa aaaacgttgg ggctacaagg gttaccactg attgccgaaa
    15721 atggcgcagt gatccagctt gctgaacaat ggcaggatat agacggtttt ccacgcatca
    15781 tctcaggtat tagccatggc gaaatcagcc aggttttaaa tacgctacgc gagaaagaac
    15841 attttaaatt cacgactttc gatgatgtcg acgatgcaac catcgccgaa tggacgggat
    15901 taagccgtag ccaggcggcg ctgacgcagc tgcatgaggc gtcggtaacg ctaatctggc
    15961 gcgacagtga cgagcgtatg gcacaattta ccgctcgtct gaacgaactg ggcttacagt
    16021 ttatgcaggg tgcgcgcttc tggcacgtcc tggatgcctc tgccggaaaa gatcaggccg
    16081 ccaactggat tatcgcgacc tatcaacaat tatcaggcaa acgcccaacc acacttggcc
    16141 tgggcgatgg gccaaacgat gcgcccttac tggaggtaat ggattacgcg gtgattgtga
    16201 aagggctaaa ccgtgaaggg gtgcatctgc atgatgagga tccgacccgc gtctggcgaa
    16261 cgcagcgtga aggaccggaa ggctggcgtg aagggctgga ccattttttc tccgctcgtt
    16321 aagcgttatc gctcgcgcat acccgattac gcccggcctg tttagcgaga taaagccgac
    16381 ggtcagccag tgactggagt tgttcaaaat cataatcacc ggtttcctcg ctgctactta
    16441 cccccaacga ggcactgatg cgtatcgtcg tactcttagc aatcaacatc tctttttcat
    16501 ttaacttcag gcgaatacgt tctgcgactt ccgcagcctc cgtcagactc gcgcctggca
    16561 gaatcacaca aaactcctca ccaccgaccc gcccggcaac gtcctgcgca cgcaaggaac
    16621 tgctaattaa tccggcagca tgagaaagaa cacggtcgcc cgcctgatga ccaaagcggt
    16681 cattaatcgc tttaaaatgg tcaaggtcga cctgaatgac agaaaaagga tgttggtgcg
    16741 tctgacacaa tttcgcgagc ggacgggctt tttcaaacag tgcgccacga ttatataaac
    16801 gggttaaggt gtcgtgccac gcctgccact gcaacgagct ttgcagaaca tacatgttgc
    16861 taaccatccg gcgaatcaca taccaggaga tgagtaacat ggtggtaaag agcgcccaca
    16921 gcagggttaa tgcaatgctg atactgccga aatcgccgcg cacgccttcg cttagcgtat
    16981 ggacgcgcac cagcacaccg tcgaaatggt ccagacgttc ccagctaaca tagcgactgt
    17041 ccatacgaat gccgccccgc gtgtcatgtt ccatcgcctg cgccagcaag gccagttcac
    17101 gaggatcaaa aatattccct gttggatggt caggattgga agaggtcaaa aatctcagct
    17161 tactgtcata gagctgatac tcaccatcga ggtttttatc gatggcgttt cttaaaaatt
    17221 gctgcatggt acgcacggga atactcatcc ccagcacgcc ataccagtag ttattactgt
    17281 ctaccggaac actgacggta acctgcggtt cagtattgct ggcgtgctcc ggttgcgaag
    17341 taaaccagcg taccgcgcgg tgacgatttt ctcgttgcga atggccgata aaccagggtt
    17401 gggtgacata gccgtaataa cgcgttggta cattgcgcgt aaacaaggtc ggctgcgtcg
    17461 aaacgtaaaa tccggcacgc gagacataca tcgcctgttc aaccatcgac gaggtgttgt
    17521 gcgccagtcg cagcaagtaa ccaacttcca gtgcagcggt aatttcattg tcgaggcttt
    17581 cattttcgcg agacaggaga ttcccctcgc tgactaatgc atccgatacg ccattgactg
    17641 acagggtgcg tcgtcggttg agttcaattt gccaggcgtg ctcgtcgcga tgctgttcga
    17701 actcggttac agcattacgc agtgaagtga aatccagtgg cgctacgaga gcttcgcgca
    17761 tgccattacg caaaaagatc agtttgtcga cgttatactg caaatgctta tcgagcgcgt
    17821 tggctacgtt ttccagatga ttacgctggc tggagatata ggcatcttcc agcactacca
    17881 cttcacgcca ggtcagcaag gtggaaaaaa gcaataccac gataaagcag agattaacga
    17941 catgaccagg ccccaggcgg cgtgcgagtt ttttcaacca gctctggttt tccatttttg
    18001 tctcgtgctg caccctgacg actcccaacc tgctttttta tgattctggc ataacctggc
    18061 gatagcgtac cgcaaaacag cgataatttt ataatggtgg catgataaga cgcgactatc
    18121 attcattgca ggcattggca caccaggccg gaagtgatcc ggcctggtgt taatgatacc
    18181 ctttcaaact tcttccgcag aacgactccc ctcccccgga acaagctcgg tttcatgaaa
    18241 agcttcccgc ttgaccccgt aaccgtcata ccaacgacac tcaaccatac cgctggagta
    18301 tccagtgaca atcatccgcg ggccgccctc tttaaccgta acttcctcac taaccataaa
    18361 gctcatactc gcctcctttt ttcgagtgaa acttgttcac cttagttgaa gatggcgaat
    18421 tttgcataat agcctgtacg agtattaatg tgcctgaccg cgcattttcg ctacggcttt
    18481 cactcccagc accacgatgc cgccgatgat aaatccaaga atcagattta aaacagtcgg
    18541 taatatcatc gccaccactg cactttgctg cccggcgaaa tgttcaatgg cgtgatgcag
    18601 cggcgcaata ccatgtacca caataccgcc gccgacgagg aacatcgcca gcgtgccgac
    18661 aatcgataac gccttcatca accagggcgc aatgatcaat aaccctttac ctaatgcctg
    18721 catcagcgcg ctggattttt ccgccagcca ataccccagg tcatcaatct taacgataac
    18781 ccctaccaga ccgtagacgc ccacggtcac taccagcgcg atgcctgaaa gcaccagcac
    18841 ctgattaagc aacggcgctt cggccacaat ccccagcgtg atggcgacga tttcggcaga
    18901 caatataaaa tcggtacgaa tcgccccttt tattttgtcc ttttcaaact tcagcggatc
    18961 ctgcgccgcc agcttctcca gacgctgctg gctctgcgcc ggatcttctt tatgtttacg
    19021 cgcctccagc atatgcagca ctttctctac tccttcaaag cagagaaacg cgccaccaat
    19081 catcaacaga ggcgtaatcg cccacgggat aaacgcactg atgatcagcg ccagcggcac
    19141 cagaatcact ttattaatca gcgatccttt cgccacgccc cagaccacgg gaagttcccg
    19201 gttggcccgc acgcctgaaa cttgttgcgc attgagcgat aagtcatccc ctaatacacc
    19261 ggcggttttc ttcgccgcca gtttgcccat cacggagata tcgtccagta gtgtggcgat
    19321 atcatcgagc aacgtcagta aactactccc ggccaaaagc attccctctc atttttattg
    19381 ttgaagggtg taagtatgaa gcaaaaacgg cagctctgat acttgcttca caggtcaaca
    19441 ttttttttac aatcaagtgc aattatatct ctcgccagca gaatgatttt cacgtttact
    19501 atgaaccacc tttttatttc caccgtgagg gattatgcgt ttccgccagt tgttaccgct
    19561 ttttggcgcg ctgtttgcgt tgtatatcat ttggggctca acctattttg tcattcggat
    19621 tggcgtggaa agctggcctc cgttaatgat ggcaggcgtt cgattcctgg cagccggtat
    19681 tttattgatg gcatttttgc tactgcgcgg acacaagctc cccccgctac gtccgctgct
    19741 caatgccgcg ctgattggcc tgttattgct ggctgtcggt aatggcatgg tgacggttgc
    19801 cgagcatcaa aatgttcctt ccggcatcgc cgccgttgtg gttgcaaccg tgcccctctt
    19861 taccctggtg cttcagccgc ctgtttggca ttaaaacgcg caaactggaa tgggtgggta
    19921 tcgccattgg gcttgccgga atcatcatgc tcaatagcgg tggaaattta agcggcaatc
    19981 cgtggggcgc gattctgatt ttaatcggct cgattagctg ggcgtttggc tcggtttatg
    20041 gctcgcgcat taccttacct gtagggatga tggcgggtgc gattgagatg ctggcggcag
    20101 gcgtggtgtt aatgatcgcg tcgatgattg caggtgaaaa actgacggcg ctcccttccc
    20161 tttcaggctt ccttgcggtc ggctatctgg cgctgtttgg ttcgattatc gccatcaacg
    20221 cttatatgta tttaatccgt aatgtcagtc cggctctcgc caccagctac gcctacgtta
    20281 acccggtggt cgcggtcttg ctgggtacgg gactgggcgg agagacgctg tcgaagattg
    20341 aatggctggc gctcggcgta attgtcttcg cggtggtact ggtcacgttg ggaaaatatc
    20401 tcttcccggc aaaacccgta gttgcgccag ttattcagga cgcatcaagc gagtaaatga
    20461 atcccctgcg tgtcgatctg cgcgctggca ccttcgccgc agatccactc ttccagacgc
    20521 tcggtaagcg cttcatccgt cagcttctcg cgcccacgta atgcacactc ccagacaatc
    20581 agtacgcgcc agccgagttc ctgcaaacga ctgatatcgc ggcgatcgcg ttcaacattt
    20641 ttacctatct tctccagcca gaactcggtt cgagtcgcag gcactttaaa cagatagcag
    20701 tgatgatgat gccagaagca gccatgggta aatatcacgc agcgatattc atcaacgaca
    20761 aaatccgggc ttccgggcag actggcgtcc tgaacgcgaa acgccaggcc ctgcccggtt
    20821 aacagactgg cgaggcgctt ctcaatcgcc gtatcacgcg tggcaatcgc gcgcatattt
    20881 ttgctgcgag tggccttatc gtgaacgtcg gccatgttgt gcctcttgct gacgcaacgc
    20941 caccgcctgt ttgatttttg gctcaagcag ttttgccacc gcggcaaaga ccggcacgac
    21001 caccgagtta ccgaactggc gataggcctg agtgtccgaa accggaatgc ggaatttcgc
    21061 ttctcccggc gcttcaaaac ccattaagcg cgcgcattcc cgaggcgtta accgacgtgg
    21121 acgatgttgc tgattcagcg gatcgtcaaa atctttctca cccgtggcca tatcccagcc
    21181 gcgatcgatt aaaatttccg cgccatcttt gtaataacgc gcagacagcg tacgcgtgac
    21241 gctttgcgga ttgttcggat aaaccattcc ataaccgaag ccgttaccgc gcgcctgatg
    21301 ttttttcgca tatcgataga ggtacttcca cagcaccggc gtcaggatat atttcgcctc
    21361 gaccatcggg tccaacagct gcgccagcgt cactcgctgt gcagggaaac attcgctgat
    21421 atcacgcaga gtaaaatcgg ctttcagatt aagatcgcga cgaaaaccca ccagcacgat
    21481 gcgttcacgg tgctgcggca gaaaatgttt accatcgatg attttcggat cgtccggccc
    21541 gttatcttct gcatcagcca cgtcatagcc cagttcgtcc agcgtctgca tgatgatgcg
    21601 gaacgtttta ccctggtcgt gacttttcag gtttttgacg ttttcgagca caaacatcgc
    21661 cggacgacgc gcgtcgataa tgcgtaccac atcaaaaaac agcgtgccct gggtatcgca
    21721 ggcaaaaccg tgcgcccgcc cgagcgagtt ctttttcgat acgccagcca gcgaaaatgg
    21781 ctgacaaggg aaaccggcca gtaaaacatc gtgttcagga atgtgttgac gaatatgttc
    21841 cgccgccgcc tcatcactca cgccttcttt atggctgagg gtgatgtcgc ggatatcttc
    21901 attaaaatga tgcgtcgccg gatcgcaata atggttggct ttataagtgc gtaccgcatg
    21961 tttgttccat tcgctggtaa acacgcattg tccgccaatc gattcaaaac cgcgacggat
    22021 gccgccaatt ccggcaaata gatcgataaa gcgaaacgca taatgcggat gatgtgccga
    22081 tggtttgggt aacaacgttt gcagatgggc gaactctttc tcacttaaac ggtgccatgc
    22141 cgagtcattc gccagcgcac gttttaaaat tgccgcgctc cagtgattct cacccacacc
    22201 attaagctgc gccaccagcg ttttaacatc ataaatttgc agcagtttct ccagcattgc
    22261 ctgtgcggca ttcccggtgc tgtatgaatc ggttactgat atattttcct gcatagattt
    22321 aaccggccat ctgagatggc cgacaggtta acataattac agccatttag ggccacgcgc
    22381 ttatcccgcg ggtgaaaacg catctatcac cttgtgatcg acaccttcat tctcccccgc
    22441 cagttcggca ctgagcttcg ccataaactc gactaaaaaa tgcgcgttgt gctgcgccag
    22501 ctgcttgcct cttgcggttt gcatggtttg cggtagcttc agcaacttag tctggaaatg
    22561 atccagcgcg tagcgtttat catcaagcgg gcgatgctgt gcgaacggat cttcaccatc
    22621 aaacagcgcc acgcccaatg cccctgaaac ggcaaacacg cgcgccaggc caatcgcccc
    22681 cagggcttcc agccgatccg catcctggac gattttagct tccgttgtta agggggcgat
    22741 ttgcgcgctg aaactgtgag cggcaatggc atgacaaacg gcctcgattt tctccgccgg
    22801 aaattgctcg aactcttcac ggagcaggcg acgcgtctct tctgccgcca ggattgaaga
    22861 acgctgcctt tgcggatgat ttttcgccag gctcacaata tcgtgaaaat aacaggcggt
    22921 taaaatcacc agcatatcga catcgtcatc tgccgccagc ttctgtgccg cgtcctggtg
    22981 ctgatgatgg tttttcaacc agttttcgaa ctgtgcctgc cagtgttgta agtccatacg
    23041 cgctccgttg ccaaagagtc gccaccttag cagtttttat tcatagcgtc tgctacgtgc
    23101 gggacgaggt cgggtgtacc aggcaatgct gcctaacaat gccccgacca gagccataaa
    23161 taaaaatccg accagtgccc ctaaccactt tcccgcggta gagcctaaat cgctttcaaa
    23221 tcccgacacc ggggcatcag ggatccggct catcacccag atgtaacgcg ccattgccca
    23281 gacgaaatag ccacaccatg tatagaaagc ccacaaagcc aatttgccgc cagggctgcg
    23341 agagagtctc tcttccatct ttgtcgaacc gtattccatt ttgagataac ccggaaaatt
    23401 cagacttagt actattatgt gataaaagtc acatttttcc acattggatg agccagtcaa
    23461 gactcaatca ttttttgaac aaatagataa attttgcgca acggcaataa ttactggaaa
    23521 aacagcaata aagatttatt tatatccttg gtaatagttt tattaattca cccaaacaat
    23581 atattagcac tgaatacaat tatacctttt ttgatttctg tgtcatgata accccgttat
    23641 tagcctttta tcgtcttgtt tatatttttt gggccggcat gatgccggct ttttttatac
    23701 cttcattaat gtgcgcgtga tcacgcctgc cgtttggcgc aacaatcatt gatactccct
    23761 a
//
LOCUS       DADMKF010000053        23489 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.054, whole genome shotgun sequence.
ACCESSION   DADMKF010000053 DADMKF010000000
VERSION     DADMKF010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23489
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.054"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(<1..673)
                     /locus_tag="JNC24_001944"
     CDS             complement(<1..673)
                     /locus_tag="JNC24_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter"
                     /protein_id="HAY9158693.1"
                     /translation="MRNKLSFDLQLSARKAAIAERITAHKIARSKVSVFLMAMSAGVF
                     MAIGFTFYLSVIADAPSSQALTHLVGGLCFTLGFILLAVCGTSLFTSSVMTVMAKSRG
                     VISWRTWLINALLVACGNLAGIACFSLLIWFSGLVMSENAMWGVAVLHCAEGKMHHTF
                     TESVSLGIMCNLMVCLALWMSYCGRSLCDKIVAMILPITLFVASGFEHCIANLFVIPF
                     AIAIRH"
     gene            complement(695..2707)
                     /locus_tag="JNC24_001945"
     CDS             complement(695..2707)
                     /locus_tag="JNC24_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HAY9158694.1"
                     /translation="MAMSDEAMFAPPQGITIEAVNGMLAERLAQKHGKASLLRAFIPL
                     PPPFSPVQLIELHVLKSNFYYRYHDDGSDVTATTEYQGEMVDYSRHAVLLGSSGMAEL
                     RFIRTHGCRFTPQDCTLFNWLARIITPVLQSWLNDEEQQVALRLLEKDRDHHRVLVDI
                     TNAVLSHLDLDDLIADVAREIHHFFGLASVSMVLGDHRKNEKFSLWCSDLSASHCACL
                     PRNMPGDSVLLTQTLQTRQPTLTHRADDLFLWQRDPLLLLLASNGCESALLIPLTFGN
                     HTPGALLLAHTSSTLFSEENCQLLQHIADRIAIAVGNADAWRSMTDLQESLQQENHQL
                     SEQLLSNLGVGDIIYQSQAMEDLLQQVDIVAKSDSTVLICGETGTGKEVIARAIHQLS
                     PRRDKPLVKINCAAIPASLLESELFGHDKGAFTGAINTHRGRFEIADGGTLFLDEIGD
                     LPLELQPKLLRVLQEREIERLGGSRTIPVNVRVIAATNRDLWQMVEDRQFRSDLFYRL
                     NVFPLELPPLRDRPEDIPLLAKHFTQKMARHMNRSIDAIPTEALRQLMSWDWPGNVRE
                     LENVIERAVLLTRGNSLNLHLNVRQSHLLPTLNEDSALRSSMAQLLHPTTPENDEEER
                     QRIVQVLRETNGIVAGPRGAATRLGMKRTTLLSRMQRLGLSVREVL"
     gene            complement(2737..3150)
                     /gene="hyfJ"
                     /locus_tag="JNC24_001946"
     CDS             complement(2737..3150)
                     /gene="hyfJ"
                     /locus_tag="JNC24_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HAY9158695.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFPCPLTEYEDWLALVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVQEPAIYLMARKIA"
     gene            complement(3143..3901)
                     /gene="hyfI"
                     /locus_tag="JNC24_001947"
     CDS             complement(3143..3901)
                     /gene="hyfI"
                     /locus_tag="JNC24_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HAY9158696.1"
                     /translation="MSPVLTQHVSQPITLDEQTQKMKQHLLQDIRRSAYVYRVDCGGC
                     NACEIEIFAAITPVFDAERFGIKVISSPRHADILLFTGAVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGREICDRLLRHLSDD
                     PTGNRVNTWLRDADDPRLNSIVQQLFRVLRGLHD"
     gene            complement(3898..4437)
                     /gene="hyfH"
                     /locus_tag="JNC24_001948"
     CDS             complement(3898..4437)
                     /gene="hyfH"
                     /locus_tag="JNC24_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HAY9158697.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACPANALTIQTDDQQNSRTWQLYLRRCIYCGRCEEVCPTRAIQLTNNFELTVTNKA
                     DLYTRATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLWAQASVCPECKQ
                     RATLLNDDTDVPLVAKEQL"
     gene            complement(4447..6162)
                     /gene="hyfG"
                     /locus_tag="JNC24_001949"
     CDS             complement(4447..6162)
                     /gene="hyfG"
                     /locus_tag="JNC24_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
                     /protein_id="HAY9158698.1"
                     /translation="MNVNSSSNRGEAILAALKTQFPGAVLDEERQTPEQVTITVKINL
                     LPDVVHYLYYQHDGWLPVLFGNDERTLNGHYAVYYALSMEGAEKCWIVVKALVDADSR
                     EFPSVTPRVPAAVWGEREIRDMYGLIPVGLPDQRRLVLPDNWPEDMHPLRKDAMDYRL
                     RPEPTTDTETYPFINEGNSDARVIPIGPLHITSDEPGHFRLFVDGEQIVDADYRLFYV
                     HRGMEKLAETRMGYNEVTFLSDRVCGICGFAHSVAYTNSVENALGIEVPQRAHTIRSI
                     LLEVERLHSHLLNLGLSCHFVGFDTGFMQFFRVREKSMTMAELLTGSRKTYGLNLIGG
                     VRRDILKEQRLQTLKLVREMRADVSELVEMLLATPNMEQRTQGIGILDRQIARDYSPV
                     GPLIRGSGFARDLRFDHPYADYGNIPKTLFTFTGGDVFSRVMVRVKETFDSLAMLEFA
                     LDNMPDTPLLTEGFSYKPHAFALGFVEAPRGEDVHWSMLGDNQKLFRWRCRAATYANW
                     PVLRYMLRGNTVSDAPLIIGSLDPCYSCTDRVTLVDVRKRQSKTVPYKEIERYGIDRN
                     RSPLK"
     gene            complement(6152..7732)
                     /gene="hyfF"
                     /locus_tag="JNC24_001950"
     CDS             complement(6152..7732)
                     /gene="hyfF"
                     /locus_tag="JNC24_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HAY9158699.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     AHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLESAWKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHILNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALAGMPPFNIFLSEFMTITAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKPPQ
                     AVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRSTW
                     HDFLPSGTASVSEKHSER"
     gene            complement(7737..8387)
                     /gene="hyfE"
                     /locus_tag="JNC24_001951"
     CDS             complement(7737..8387)
                     /gene="hyfE"
                     /locus_tag="JNC24_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HAY9158700.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            complement(8399..9751)
                     /locus_tag="JNC24_001952"
     CDS             complement(8399..9751)
                     /locus_tag="JNC24_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005136530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit D"
                     /protein_id="HAY9158701.1"
                     /translation="MGVLFAALTTLCMLSLISAFYQADKVAVTLTLVNVGDVALFGLV
                     IDRMSTLILFVVVFLGLLVTIYSTGYLTDKNREHPHNGTNRYYAFLLVFIGAMAGLVL
                     SSTLLGQLLFFEITGGCSWALISYYQSDKAQLSALKALLITHIGSLGLYLAAATLFLQ
                     TGTFALSAMSELHGDARYLVYGGILFAAWGKSAQLPMQAWLPDAMEAPTPISAYLHAA
                     SMVKVGVYIFARAIIDGGNIPHVIGGVGMVMALVTILYGFLMYLPQQDMKRLLAWSTI
                     TQLGWMFFGLSLSIFGSRLALEGSIAYIVNHAFAKSLFFLVAGALSYSCGTRLLPRLR
                     GVLHTLPLPSVGFCVAALAITGVPPFNGFFSKFPLFAVGFALSVEYWILLPAMILLMI
                     ESVASFAWFIRWFGRVVPGKPSEAVADAAPLPGSMRLVLIVLIVMSLISSVIAATWLQ
                     "
     gene            complement(9727..9837)
                     /locus_tag="JNC24_001953"
     CDS             complement(9727..9837)
                     /locus_tag="JNC24_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoin racemase"
                     /protein_id="HAY9158702.1"
                     /translation="MENLALTTLMLPFIGALVVSFSPQRRAAEWGFCSPH"
     gene            complement(9854..10801)
                     /gene="hyfC"
                     /locus_tag="JNC24_001954"
     CDS             complement(9854..10801)
                     /gene="hyfC"
                     /locus_tag="JNC24_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HAY9158703.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEDAPTSSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLATGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMAALFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            complement(10812..12830)
                     /gene="hyfB"
                     /locus_tag="JNC24_001955"
     CDS             complement(10812..12830)
                     /gene="hyfB"
                     /locus_tag="JNC24_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
                     /protein_id="HAY9158704.1"
                     /translation="MDALQLLTWSLILYLFASLASLFLLGLDRLAIKLSGITSLVGGV
                     IGIISGITQLHAGVTLVARFATPFEFADLTLRMDSLSAFMVLVISLLVVVCSLYSLTY
                     MREYEGKGAAAMGFFMNIFIASMVALLVMDNAFWFIVLFEMMSLSSWFLVIARQDKTS
                     INAGMLYFFIAHAGSVLIMIAFLLMGRESGSLDFASFRTLSLSPGLASAVFLLAFFGF
                     GAKAGMMPLHSWLPRAHPAAPSHASALMSGVMVKIGIFGILKVAMDLLAQTGLPLWWG
                     ILVMAIGAISALLGVLYALAEQDIKRLLAWSTVENVGIILLAVGVAMVGLSLHDPLLT
                     VVGLLGALFHLLNHALFKGLLFLGAGAIISRLHTHDMEKMGALAKRMPWTAAACLIGC
                     LAISAIPPLNGFISEWYTWQSLFSLSRVEAVALQLAGPIAMVMLAVTGGLAVMCFVKM
                     YGITFCGAPRSTHAEEAQEVPNTMIVAMLLLAALCVLIALSASWLAPKIMHIAHAFTN
                     TPPVTVASGIALVPGTFHTRVTPSLLLLLLLAMPLLPGLYWLWCRSRRAAFRRTGDAW
                     ACGYGWENAMAPSGNGVMQPLRVVFSALFRLRQQLDPTLRLNKGLAHVTARAQSTEPF
                     WDERVIRPIVSATQRLAKEIQHLQSGDFRLYCLYVVAALVVLLIAIAV"
     gene            complement(12830..13447)
                     /gene="hyfA"
                     /locus_tag="JNC24_001956"
     CDS             complement(12830..13447)
                     /gene="hyfA"
                     /locus_tag="JNC24_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HAY9158705.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     LTAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAPAQYVFQAEGSLKDGEENVPPQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGEDPLSLTQEQR"
     gene            complement(13700..14170)
                     /gene="bcp"
                     /locus_tag="JNC24_001957"
     CDS             complement(13700..14170)
                     /gene="bcp"
                     /locus_tag="JNC24_001957"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HAY9158706.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            complement(14170..14742)
                     /locus_tag="JNC24_001958"
     CDS             complement(14170..14742)
                     /locus_tag="JNC24_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HAY9158707.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            14888..15766
                     /gene="dapA"
                     /locus_tag="JNC24_001959"
     CDS             14888..15766
                     /gene="dapA"
                     /locus_tag="JNC24_001959"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HAY9158708.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLELADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            15783..16817
                     /gene="bamC"
                     /locus_tag="JNC24_001960"
     CDS             15783..16817
                     /gene="bamC"
                     /locus_tag="JNC24_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HAY9158709.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            17030..17743
                     /gene="purC"
                     /locus_tag="JNC24_001961"
     CDS             17030..17743
                     /gene="purC"
                     /locus_tag="JNC24_001961"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HAY9158710.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMLNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            17911..18759
                     /locus_tag="JNC24_001962"
     CDS             17911..18759
                     /locus_tag="JNC24_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HAY9158711.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHNMQ
                     QQGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGDPAQCN
                     TFGKSI"
     gene            18774..20789
                     /locus_tag="JNC24_001963"
     CDS             18774..20789
                     /locus_tag="JNC24_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(Met) cytidine acetyltransferase"
                     /protein_id="HAY9158712.1"
                     /translation="MAELTALHTLTAQMKREGIRRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQPDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFNDENFTHTPQGNIVISAFEQTLWRSDPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQELSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGVGQQLIASALQYRPGLDYLSVSFGYTGELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGETLPVD
                     PLNNAVLSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNTSDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNEVRTERLRDRITQWQFFH"
     gene            20863..21561
                     /gene="ypfH"
                     /locus_tag="JNC24_001964"
     CDS             20863..21561
                     /gene="ypfH"
                     /locus_tag="JNC24_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HAY9158713.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIEMVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDEAL
                     SGGKPGDDDVIEMM"
     gene            complement(21671..21871)
                     /locus_tag="JNC24_001965"
     CDS             complement(21671..21871)
                     /locus_tag="JNC24_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HAY9158714.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(21899..23026)
                     /gene="dapE"
                     /locus_tag="JNC24_001966"
     CDS             complement(21899..23026)
                     /gene="dapE"
                     /locus_tag="JNC24_001966"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HAY9158715.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIDRLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            complement(23030..23386)
                     /locus_tag="JNC24_001967"
     CDS             complement(23030..23386)
                     /locus_tag="JNC24_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HAY9158716.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLCAPGKPM
                     LLGFSDSSYQQFFHEV"
BASE COUNT         5887 a         6233 c         6281 g         5088 t
ORIGIN      
        1 aatggcgaat ggcaatggcg aacggaatca caaacaaatt ggcgatacag tgctcaaacc
       61 cactggcgac aaacagggtg atgggcaaaa tcatggcgac gattttgtcg cataatgaac
      121 gcccgcaata actcatccac agcgccagac aaaccatcag attgcacata atgccgaggc
      181 tgacagattc agtaaatgta tgatgcattt tgccctcggc gcagtgtaaa accgcgactc
      241 cccacatcgc gttttcactc atcaccagcc cggaaaacca gattaacaaa ctgaaacagg
      301 caatacctgc cagattaccg caggccacca gaagtgcgtt aatcagccaa gttcgccaac
      361 taataacgcc ccgacttttt gccatcaccg tcattaccga cgaggtgaac aggctggtgc
      421 cacaaaccgc cagcaaaata aagccgagtg taaagcaaag gccgcccacc agatgggtta
      481 atgcctgtga agacggggca tcggcgataa cggaaaggta aaaagtaaat ccaatcgcca
      541 taaacacgcc agcggacatc gccattaaaa agaccgacac tttgctgcgg gcaattttat
      601 gggcagtaat ccgttcagcg attgccgctt ttctggcgct caactgcaag tcgaaagaga
      661 gtttgtttct catgcatact cctgcaaaag cagattacaa cacctcgcga accgagagcc
      721 ccagacgctg cattcgtgac agcagcgtgg tgcgcttcat ccctaatcgt gtcgccgcgc
      781 cacggggccc ggcaacaatg ccattggttt ctcgcaatac ctgaacaatg cgctgacgtt
      841 cttcttcgtc attctctggc gtcgtcgggt gcagcaactg cgccattgaa ctgcgaagcg
      901 ctgaatcttc atttagcgtc ggcagtaaat ggctttgtcg gacattaaga tgtaaattca
      961 gactgttacc gcgagtcagc agtaccgctc gctcaatcac gttttccagc tcgcgcacgt
     1021 tgcccggcca gtcccacgac atcaactggc gtagcgcctc ggtcgggatg gcgtcaattg
     1081 aacgattcat atggcgcgcc attttttgcg tgaaatgctt tgctaaaaga gggatatctt
     1141 ccggacggtc gcgtagcggc ggcaattcca gtgggaagac attcaggcga taaaagagat
     1201 cgctgcgaaa ctggcgatct tcaaccattt gccacaaatc acggttggtg gcggcaatga
     1261 ctctgacatt caccgggatc gttctactcc cgccgagacg ctcaatctcc cgctcctgca
     1321 atacgcgcag cagtttaggc tgaagttcta acggcagatc gccaatttca tcaagaaaca
     1381 acgtgccgcc atcggcaatt tcaaaacgac cacgatgggt attaatcgca ccagtaaacg
     1441 cccctttgtc atgaccgaat aactcacttt ccagaagact ggcggggatg gcagcgcagt
     1501 tgattttgac cagcggcttg tcgcgtcgcg ggctaagttg atggatcgct ctggcgatca
     1561 cctctttgcc ggttccggtt tcaccacaaa tcaacactgt actgtcgctc ttcgccacaa
     1621 tatctacctg ctggagcagg tcttccattg cctggctttg atagataatg tcaccgacgc
     1681 ccagattcga aaggagctgc tcgctaagct ggtggttttc ttgctgcaaa ctttcctgca
     1741 aatcggtcat gctacgccag gcatcggcat tgccaacggc aatagcgatg cgatcggcta
     1801 tgtgttgtag tagctgacag ttttcctcac taaagagagt ggaagaggta tgcgccagca
     1861 acaatgcacc cggtgtatgg ttgccaaagg taagcggtat aaggagcgca gattcgcagc
     1921 cgttagatgc aagtaagagt aataacgggt cgcgttgcca gagaaacaga tcgtctgcac
     1981 ggtgcgtcaa ggtcggttgt cgggtttgta gcgtttgtgt cagcaataca ctgtcgccag
     2041 gcatattgcg tggcagacac gcacaatgtg aggcagaaag atcgctgcac cacaggctaa
     2101 acttctcgtt ctttcgatga tcgcccagta ccatactgac tgaagccaga ccgaaaaaat
     2161 gatggatctc acgagcgacg tcagcgatca gatcgtcgag atcaagatgt gacagcactg
     2221 cattagtaat atcaaccagt acccgatgat gatcgcgatc tttctccagc aaacgcagcg
     2281 ccacctgctg ttcttcatca ttgagccatg attgcagaac cggggtgatt atccgcgcca
     2341 gccagttaaa cagtgtgcaa tcctggggag taaaacggca gccgtgggtg cgaataaagc
     2401 gtagctccgc cattccacta ctgccgagaa ggacggcgtg acgcgaataa tcgaccatct
     2461 cgccctgata ctctgttgtt gccgtcacat cgctgccatc atcatggtag cggtaataga
     2521 agttgctttt gagaacatgc agttcaataa gttgtaccgg gctgaacggc ggcggcagcg
     2581 ggatgaaggc gcgtaataaa gacgccttac cgtgtttctg cgctaaccgc tccgcgagca
     2641 ttccgtttac cgcttcaatt gttattcctt gcggcggggc aaacatcgcc tcgtctgaca
     2701 tagccatcaa cagtccttat taatgagaaa tccttctcac gcaatttttc tggccatcag
     2761 atagatggcc ggttcctgca caatttcatc caacagatga atgaattgaa tgctacgcgc
     2821 ctgccacaca gacgtcgcgt catccgccag tggcgcgaat gcccgggtca acagggcggt
     2881 gtggctggcg tcaataacaa tctcaccaaa agtcatcacc cccagcatct tacgtcgggc
     2941 ttgctcctct tcgaccagtg caagccaatc ttcgtattcc gtcagtgggc aggggaaggc
     3001 gacattcaga caatcaatca cgccaacgtg atgaccgata gccagcgagt aatacattac
     3061 ctgagagctg tgttccggca tctcgtcgct actggcgaca aacttttttc gcagcgtcca
     3121 gaaaacaatt tccccgcact cttcagtcat gtaacccccg gagtacgcga aagagttgct
     3181 gaacgatact attgagacgt ggatcgtcgg catcgcgcaa ccaggtgtta acccgatttc
     3241 ctgtaggatc gtcgcttaaa tggcgcagga gccgatcgca aatttctcgc ccctgacgat
     3301 agcccgccag ccgccgcgct tctcgctcaa tggcgatacg ctgtgatggc gggatctgcg
     3361 gccacaacgg ttgcatagtc accccggtgg gatcgcgata atccaccgcg tgaatcttct
     3421 gttgcagcaa accgagcgcc acggcgaaac cgtgaatggt ggcggccggt gttggcgggc
     3481 agccggggat ccaaacatca atggggacaa tggtgtcgct gccgccccag acgctgtaga
     3541 gatcgtggaa aataccgccg ccgacaccgc acgcgccgta ggaaacacaa attttatgat
     3601 cgggggcaga ctcatacgcc cgaagtgcag gcatacgcat cgcccgggtg actgcgcctg
     3661 taaataacaa aatatcggcg tgacgcggtg atgaaataac cttaatgcca aaacgttctg
     3721 cgtcgaatac tggtgtgatg gcggcaaaaa tttcgatttc acaggcgttg cagccgccgc
     3781 aatcgacgcg ataaacgtaa gccgagcgac ggatatcctg tagcaaatgc tgcttcatct
     3841 tttgcgtttg ctcgtccagc gtgatgggct ggctgacatg ttgtgtaagc actggactca
     3901 tagctgctcc ttagccacca gcggtacatc tgtatcgtcg ttgagcagcg tcgcgcgttg
     3961 tttgcactcc gggcagacgc tcgcttgcgc ccataacatt tcgcggtttt gtggcgcatt
     4021 ttgttgctgt gccaacaatt cagcagccag tgcgacggtt ttttgcgggg caaacgggcg
     4081 ttcgcaacgg ctgcaacgtt gtagatggaa cgtcgcgcgg gtatagagat cggctttatt
     4141 ggtgacggtc agttcaaagt tattggtaag ctggatggct ctggtcgggc acacttcttc
     4201 acaacgtccg cagtaaatac aacgcctcag atagagctgc caggtgcgcg aattttgctg
     4261 gtcgtcggtc tggatagtca gcgcatttgc cggacaagca caggcgccgc aggcaataca
     4321 ttggctgggc atcaggtccg gtttcccgcg aaagcctggg ctgacctcca gtggcgcgaa
     4381 gggatatttc accgtcgcgg ttccggcgcg cataatagtt ttcagtaact tcagcatctt
     4441 ctgtccttac ttcagcggcg aacggttacg atcaatgccg tagcgttcga tctctttata
     4501 cggcacggtt tttgactggc gcttgcgcac atctaccagc gtcacacggt cggtacagga
     4561 gtagcaggga tcaaggctac cgataatcag cggtgcgtca gaaacggtat tgccgcgcag
     4621 catgtaacgc aacaccggcc agttggcgta ggtggcggca cggcagcgcc agcggaacaa
     4681 tttttggtta tcaccgagca tgctccagtg cacgtcttca ccgcgtggcg cttcaacaaa
     4741 gcccagcgcg aatgcgtgag gtttatagct aaagccttcg gtcagcagtg gggtatccgg
     4801 catgttgtcg agggcaaatt ccagcattgc cagcgaatca aacgtctctt tgacacggac
     4861 catcacgcgg gagaaaacat cgccgccggt aaaggtaaac agtgtttttg gaatattgcc
     4921 gtagtcggcg taggggtgat caaagcgcaa atcacgggca aaaccactgc cgcggatcag
     4981 cggccctaca gggctataat cacgggcgat ttgtcggtcg agaatgccaa tgccctgagt
     5041 gcgttgttcc atattcggcg tggcaagcag catctctacc agctccgaca cgtcggcgcg
     5101 catctcgcgc accagtttca gcgtttgcag acgttgctct ttgagaatat cgcggcgaac
     5161 accaccaatc agattcagac cgtaggtttt acgcgacccg gtcagcaatt ccgccattgt
     5221 catcgacttt tcccgcacgc ggaaaaattg cataaagccg gtatcaaatc caacaaaatg
     5281 gcaggagagg ccaaggttga gcaaatgact gtgcagacgt tcgacttcca gcagaatcga
     5341 gcgaatagta tgcgctcgtt gcggcacctc aatccccagt gcattttcaa ccgagttggt
     5401 ataggccaca ctgtgggcaa aaccgcaaat cccacacacg cgatccgata agaaggtcac
     5461 ttcgttgtag cccatccgcg tttctgccag tttctccatg ccgcgatgga cataaaacag
     5521 gcggtaatca gcatcgacaa tttgctcgcc atccacaaac aagcggaagt gacccggttc
     5581 atcggaggtg atatgcagcg ggccaatagg gatcacccgc gcatcgctat tgccctcatt
     5641 gataaacgga tacgtttcgg tatcagtcgt cggttcaggg cgcagtcgat aatccatcgc
     5701 atctttgcgc agcggatgca tatcttccgg ccagttatcg ggcaacacca ggcgacgctg
     5761 atccggcagg ccaaccggaa tcagcccgta catatcgcga atttctcgct cgccccagac
     5821 cgcggcaggg acgcgcggtg tgactgacgg aaactcccga ctgtcggcat cgaccagtgc
     5881 cttcaccacg atccagcatt tttcggcccc ttccattgaa agggcataat aaaccgcgta
     5941 atgaccgtta agtgtccgct cgtcgttgcc aaacaggact ggaagccagc catcatgttg
     6001 ataataaaga taatgtacaa cgtcaggcag cagattgatt ttcaccgtaa tggtgacctg
     6061 ttcaggcgtt tgtcgctctt catccagcac cgcgccgggg aactgcgttt tcagggcggc
     6121 gagaatcgct tcgccacgat ttgacgatga attaacgttc actgtgtttc tccgaaacag
     6181 atgcggtgcc tgaaggcaaa aaatcatgcc aggtgctacg ttgtgcaggc aggtcgtgcg
     6241 tccctgagag gactatagtg gaagcgcccg ccaggatcct gatgacaggt tgtggaatat
     6301 gcgttcccat cgccagcatc atgaccagca gaatcaccat tggcgaggtg gtcaaccagc
     6361 cgagatcacc ccggttaacg gcctgcggcg gtttcgccat taacacccgc gctgccatcc
     6421 gtaccaggcc cgccagcacc agcgttaaca gcaataacag caggacgata atcagcaggt
     6481 gattacgtgc cagtccggcg gtaatggtca taaattcgct aagaaaaatg ttgaagggcg
     6541 gcatccctgc cagcgccagc gcaccgccgc caaacagcac ggcggtaaat ggcatgattt
     6601 tgagcatccc acagacgacg ttgagatcgc gcgtgccgta cttgagcagt acattgccgg
     6661 aaccgcagaa cagcagcgtt tttgccagac tgtggtttaa gatgtgcagc agcgcggcaa
     6721 aaattcccag cggcccgcca atgcctagcg ccaccgcgac cagccccatg ttctccacgc
     6781 tggagtacgc cagcagacgc ttaatgtccc gctgtaccag aatgaaaaat gccgccacgg
     6841 caaccgacaa catgccgaag atgagcaaca accggttggg gaaatcgctg ccgatggctt
     6901 ggcaaataat gatgtaatag cgaatcagca ccaacagcgc gcagttcagc aatacggcgg
     6961 agagcagggc gctgaccgga ctcggcgctt cactgtgagc atccggcagc caggcgtgca
     7021 tgggaaatag cccggttttg gtaccaaagc caattagcac aaacacaaag gccaacagca
     7081 ttaatgttgg gtcaagcaag gacgattgct taagaacctc gctccagaat atcgccattt
     7141 ctgcctgcgg cataacgctg gcggcgttgg cgtataccag cacggtaccg aacagaccaa
     7201 aagcgacacc aacagtacaa ataatgatgt acttccatgc tgattccagc gatgaacgct
     7261 gaccgtaaat gcctaccaga aacgccgagc ttaaggtggt ggcttcgatc gccgcccaca
     7321 tcacaatcag gttattgctg gtaacaacca gcagcatggt gaacaaaaac agatggaaga
     7381 aaccgtagta atcgcacagc gtaacgggtg aaagctcgcc gtgtgccact tcatgacgca
     7441 tgtagccaat cgagtaaaca ccggtgagaa agccaatcac accaagaatg gcgaggaaca
     7501 aaccgcccag accatcaata tgaagccaca gtcccgcagc gaatatttct cctgcatcag
     7561 cggcagtttg gaccacccag agtgccagaa tcagcagcag tgtgatccct aagctatgta
     7621 atacagtcac cgtacaagtc gcagagagcc cccgtttccg gcaggcaaaa cagagcagcg
     7681 aaaaaagcag cggcgtgagc aggagtaaag cgaacatcac agaataactc atctcattat
     7741 cccttcagcg cggtcaagtt gttcacgtcc agcgtgccgt gggtacgcca tatttttctt
     7801 gccagtaaca ccatcacaat gacggcgaag atggcgtcgg tagcgatacc tatttccacc
     7861 agttccggtg ctcgccaggc aagaagcgcc agcaccagat gggagccgtt ttccatcagg
     7921 cagtaaccaa aaatttgccg caggatattg cgctggctga caatgcacag caggccaagc
     7981 agaaaatgac ctaacgctac cgccagcgcc ggtttcagcc cggtagccat cggtagcttc
     8041 acgggctgaa cgacaaatgc gcaaagcagg acaattaacg ccgcgagcag tgccatcatt
     8101 gccggaccga ataacgcttt ttccgggatg ttctggggaa tatttcgtgc agcgtaagtc
     8161 atgattaacg gtaccagcag cactttggtg ataaaggcgc tggcggacca gatcagcagt
     8221 tgctctgcgg cgaacaggca cgagagagtg gcgaaaatag acaccagcac cagcgactgg
     8281 caggcgtaaa atccgcatga caggcgatag cttttgacgc taatcacaaa cagcgatgtc
     8341 agcatcatca gtcccgccag attatttacg atcatagaac cggtcattca tcatctcctt
     8401 actgcaacca ggtcgcggcg attacgctgg aaatcagcga catcacaatc agtacaatca
     8461 acaccaggcg cattgaccct ggcagcggtg cggcatcggc gacggcctcg ctcggtttgc
     8521 ccggcacgac gcgaccaaac cagcgaataa accaggcgaa actggcgacc gattcaatca
     8581 tcagcagaat catggcgggc agcaggatcc agtactccac tgacaacgca aaaccgacgg
     8641 caaacagcgg gaatttactg aagaagccgt tgaacggcgg cacaccggta atcgccagcg
     8701 ctgccacgca gaaacccacg cttggcaacg gcagggtgtg caatacgcca cgcagacgcg
     8761 gcaacaagcg cgtgccgcag ctgtaactca gcgcacctgc tacaaggaaa aacaggcttt
     8821 tagcgaacgc gtggttgacg atgtaggcga tgctaccctc cagcgccagc cgcgagccga
     8881 agatggagag cgacaagccg aagaacatcc agccaagttg agtgatggtc gaccaggcca
     8941 gcaaccgctt catatcctgc tgtggcaaat acatcagaaa gccataaaga atggtgacca
     9001 gcgccatgac catgccaacg ccgccaatca catgcgggat attgccgccg tcgataatgg
     9061 cgcgggcaaa aatgtaaacg cccactttca ccatcgatgc ggcgtggaga taggcactga
     9121 tcggtgttgg cgcttccatt gcatccggta gccacgcttg catcggtagc tgggccgatt
     9181 tcccccacgc ggcaaacagg atgccgccat aaaccagata acgtgcgtcg ccgtgtaact
     9241 cgctcatcgc gctaagcgca aacgttccgg tctgcaaaaa cagcgtggcg gcggcaagat
     9301 acaaccccaa cgagccgata tgagtgataa gtaacgcttt tagtgctgaa agctgtgctt
     9361 tatcgctctg gtaataactg atcaacgccc aggagcagcc gcccgtaatt tcaaaaaaca
     9421 acaactgacc gagcagcgtc gaggagagta ccagtcccgc catcgcgccg ataaacacca
     9481 gcaaaaatgc gtaataacga ttcgtgccgt tatgcgggtg ttcgcgattt ttatccgtca
     9541 gataacccgt ggagtagatc gtgaccagca aaccgagaaa caccaccaca aacagaatca
     9601 gcgtactcat gcgatcaatg accaagccaa acaatgccac atcccccacg ttgaccaacg
     9661 tcaacgtgac ggcaacttta tcggcctgat aaaacgcgga gatcagcgac aacatgcaca
     9721 gcgtggtcag tgcggcgaac aaaaccccca ttcggcggcc cgacgttgtg gcgaaaacga
     9781 aacgaccagt gcgccgataa aaggcagcat taacgtcgtc agagcaagat tttccatatt
     9841 ccgtcagtgc tccttacaga ccggttaacc agaagaccca tgcaagcgca gcaaggctga
     9901 agccaagcca ggtcacatgg tgaatgagta aaaaacgccc gcgtgccagc gtgttttctg
     9961 cgattgaggc cagtacaaaa atcagcaaaa ccttgagcag cgtaacgaca agtgaagtca
    10021 gcaggcaggc gagagaaagt tcttgcgcgc gcccaaaggg cagaaacagg gccacaaaca
    10081 gtgctgccat cacgacctgt ttcagcccca gcccccattt cgctagcgcc agcccggcac
    10141 cggaatattc ggtcagcggg ccttcctgta attcctgttc tgcttcagca acatcaaagg
    10201 gaatttttcc catctcaatg aagcaggcaa aaccacaggc cagtaacgcc agtacggtgg
    10261 ttagcggcga gttccagccc gttgccagcg tattgctgat catctcgata tgcgtggaac
    10321 ctgctatcag cgccaatacc agcagtgaga gaatgagcat gggttcgacc agaatgccga
    10381 gcgtcaactc gcgactggca ccgactcccg caaacgggct tccggtatcc agccctgaaa
    10441 gagcaaagaa aaaacgaaac agggcaagaa gatagataag ggtgatcaga tcgccgccgc
    10501 ccgcaaaagg ggaaacggta ataaatagtg gtagggccat cgccagcacc agcatgctgc
    10561 tgattaatac ccacggcatc aggcggaaca tcagacctga agatgtcggc gcatcttcct
    10621 ggcgtttaaa cagtttgtgg atatcgcgat aatcctgcca gatccccggc ccgcggcggg
    10681 agtgcatacg cgcgcgtatc tgccgggaaa tacccgtaaa aagtggggtt agcatcagca
    10741 gaatcacggc ctgtgctaac gcaaaaatga ccagatatcc gtcgcaaaga gtttgtctca
    10801 tggtgatttc cttagacggc aatagcgatt agcagcacaa ccagtgcggc gaccacatac
    10861 aggcaataga gacgaaagtc gccgctttgc agatgctgta tttctttggc cagccgttgg
    10921 gtggcgctca cgatggggcg gatcacccgc tcatcccaga agggttctgt gctctgagcc
    10981 ctggcggtga cgtgcgcaag acctttattt agcctcagcg tagggtcgag ctgttgtcgt
    11041 agacgaaata gcgcagaaaa gaccacacgc agcggctgca tcacgccatt gcctgacggg
    11101 gccatcgcat tttcccagcc gtagccgcat gcccaggcat ctcctgtgcg acgaaacgct
    11161 gcgcggcgcg aacgacacca cagccagtaa aggccaggca gcaaaggcat cgccagtagt
    11221 aacagcaaca gtaatgaagg agtgacccgt gtatgaaacg tgccgggtac aagtgctatt
    11281 ccgctggcga cagtgacggg aggggtattg gtaaacgcat gggcaatgtg cattatcttc
    11341 ggtgccagcc aactggcact aagcgcaatt aatacgcaga gtgccgcgag cagtagcatg
    11401 gcgacgatca tcgtatttgg cacttcctgt gcctcttcag cgtgtgtgct gcgcggcgca
    11461 ccacagaaag taataccgta cattttgacg aagcacatta ctgccagccc accagtgact
    11521 gccagcatta ccatagcaat aggacccgca agttgtagcg ctacggcttc cacacgactt
    11581 agtgagaata gcgactgcca ggtgtaccat tcgctgataa aaccattcag cggaggaatg
    11641 gctgatatcg ccaggcaacc aatcaggcat gctgcggctg tccacggcat ccgtttcgcc
    11701 agtgccccca ttttttccat gtcgtgggta tgcaaacgcg aaataatcgc acccgcgccg
    11761 agaaatagca gccctttgaa aagcgcatgg ttgagcagat gaaacagtgc gccgagcagt
    11821 ccaaccacgg tgagcagcgg gtcgtgcagt gacagaccga ccatcgccac accgaccgcc
    11881 agcaaaataa tgccgacgtt ttcgacggta ctccaggcca gcagccgttt gatatcctgt
    11941 tccgccagtg catacagcac gcccaggagc gcggagattg cgccgatcgc catcaccaga
    12001 atgccccacc acagcggcaa acccgtttgc gccagcagat ccatcgccac tttcaggatg
    12061 ccgaaaatac cgattttgac cattacgcca gacatcaacg ctgaagcgtg cgatggtgcg
    12121 gcagggtgag cgcgcggcaa ccagctgtgc aacggcatca tcccggcttt cgcgccaaaa
    12181 ccgaaaaagg ccagcaggaa caccgccgac gccagccccg gagaaagtga aagcgtgcgg
    12241 aaactggcaa aatcgaggct gccgctttcg cgccccatca gcaagaaggc gatcattatc
    12301 agcaccgatc cggcgtgggc gataaaaaag tagagcatgc cagcgttgat cgacgtttta
    12361 tcctgcctgg caatgaccag aaaccaggaa gacagcgaca tcatttcaaa cagcacgatg
    12421 aaccaaaaag cgttgtccat caccagcagg gcaaccatcg atgcgatgaa aatattcata
    12481 aagaagccca tcgccgccgc gcctttgccc tcgtattcgc gcatataagt caatgaatag
    12541 agagaacaaa ccaccaccag caaggagata accagcacca taaatgccga gaggctatcc
    12601 attcgcaggg ttaaatcggc aaattcaaaa ggcgtggcaa aacgggcgac taaagttacg
    12661 cctgcatgta attgcgtaat tccgctgatg atgccaatca cgccgcccac cagcgatgtg
    12721 atgccggaaa gcttaatagc cagtctgtcc agaccgagta aaaacagcga agccagactg
    12781 gcaaacagat agaggatcag cgaccaggtt aataattgca gggcatccat tagcgttgct
    12841 cctgagtgag ggaaagggga tcctccccgc cattggcaaa ccagcttgcg gcaaggcgct
    12901 gtttttcttt catctggcgt tgcaggctat cgtcggtgat cagccgtaac gcctgattcg
    12961 ggcaagcgcg aacgcaggcc ggaccttctg gcaagaaatc acacaggtcg catttcaccg
    13021 cgacggtctg gacacccggt tcccagcgca gcaacgcatg ttggggcggc acgttttctt
    13081 cgccgtcttt gagtgagccc tcagcctgaa aaacatattg cgcaggggca ttcaccggac
    13141 ggcttcctga agcgctgatt gcgccaaatg ggcagaccac ggcgcaaagc ttgcagccaa
    13201 tacagaggct ttcgttgagt tggatcgcat catccctctg agagatggca ttgaccgggc
    13261 agacctgcag gcaaggggct tcctcacagt gatgacacac gacaggggca gtgagtgttg
    13321 acgtcttcgc cagggccagg cgcgggtgtt gctgtaaacc ttgcgtttta tgcacgtccg
    13381 aacaggcagc gagacaggta ttacatcctg tacaccacag tggttcggcc accacaaagc
    13441 ggttcatgtc ggctccttga aaatgatatg aaacattgca tttcatattg cataaaggat
    13501 gctattctga ttaactcaat atattcaata agttatgtat taactggatt ctgaacggta
    13561 caaaaatgat ggtcattaac gttcagcaaa aaatggattc gacacccgtg tcgcagggga
    13621 aagcgagaat gtgatcttga tcacgagaag tgaggtatgc tggccgcaag cgcagccagc
    13681 acggaatgga gcaaagtaat caggcgtgtt ctttcagcca gttcagcaca acgtcgtggt
    13741 gattgctggt tttgaaatcg tcaaagacat gttcgatttt gccatcagcg tcaatcagga
    13801 agctgatgcg atgaatgcca tcgtaggttt tgcccatgaa ggacttttca ccccagacgc
    13861 cgaattgttc gcacacctgg tggtcctcat cagacaggag cgtaaagtta agcagctctt
    13921 tttccgcaaa acgggagagt ttttcgggtt tatcggtgct gatacccagc acatcaacgc
    13981 ccgctttttt caactcatcc atgttatcgc gtaagccgca ggcctgtacg gtacagccgg
    14041 gggtcatggc tttcgggtag aaataaacca gaacacgctg tccctggaag tcggtcaaat
    14101 taacttgttc tccgtcttga tccggcaagc taaatttcgg tgcgatatca ccggctttca
    14161 gtggattcat tacttaactc catcctgttc atcatgttgg gaataattga cgacgttaat
    14221 actgccttgt gcattgagtt ctgtacatag ggctttgaac gcttgctcaa tatttgctgc
    14281 gtccgcagat gcggggctgt gggcggttat ctgaatatgc aactgcgcag cccgttcatt
    14341 ttcagcaggt tgcgtgcgcg acaccagctc cgcaatgttc atatgatgcg cgtcgaaaag
    14401 tgctgtgaag cgttcaatta aatgcgggga gtctgccaca tcgacctgaa cccagacaga
    14461 tgctggcatt ggcggacgcg gacgcgccgt cgtgcgcttc atcacgatta aaagatccag
    14521 ctcggcacct ttcaacggta acgttgattc aatcagagta atggcattcc atgaaccgga
    14581 aagcagcatg ataaacgtga actcttctcc cagcatcgcc aggcgactgt cttcaatatt
    14641 acagccgcaa ctactgacat gacgggtgat ggtgttcaca attccagggc gatcggcacc
    14701 caatgcagtg atcaccagat aatgttgcga tgacagtgtc aaactggtta ttcctttaag
    14761 gggtgagttg ttcttaagga aagcataaaa aaaacatgca tacaacaatc agaacggttc
    14821 tgtctgcttg cttttaatgc cataccaaac gtaccattga gacacttgtt tgcacagagg
    14881 atggcccatg ttcacgggaa gtattgtcgc gattgttact ccgatggatg aaaaaggtaa
    14941 tgtctgtcgg gctagcttga aaaaactgat tgattatcat gtcgccagcg gtacttcggc
    15001 gatcgtttct gttggcacca ctggcgagtc cgctacctta aatcatgacg aacatgctga
    15061 tgtggtgatg atgacgctgg agctggctga tgggcgcatt ccggtaattg ccgggaccgg
    15121 cgctaacgct actgcggaag ccattagcct gacgcagcgc ttcaatgaca gtggtatcgt
    15181 cggctgcctg acggtaaccc cttactacaa tcgtccgtcg caagaaggtt tgtatcagca
    15241 tttcaaagcc atcgctgagc atactgacct gccgcaaatt ctgtataatg tgccgtcccg
    15301 tactggctgc gatctgctcc cggaaacggt gggccgtctg gcgaaagtaa aaaatattat
    15361 cggaatcaaa gaggcaacag ggaacttaac gcgtgtaaac cagatcaaag agctggtttc
    15421 agatgatttt gttctgctga gcggcgatga tgcgagcgcg ctggacttca tgcaattagg
    15481 cggtcatggg gttatttccg ttacggctaa cgtcgcagcg cgtgatatgg cccagatgtg
    15541 caaactggca gcagaagggc attttgccga ggcacgcgtt attaatcagc gtctgatgcc
    15601 attacacaac aaactatttg tcgaacccaa tccaatcccg gtgaaatggg catgtaagga
    15661 actgggtctt gtggcgaccg atacgctgcg cctgccaatg acaccaatca ccgacagtgg
    15721 tcgtgagacg gtcagagcgg cgcttaagca tgccggtttg ctgtaaagtt tagggagatt
    15781 tgatggctta ctctgttcaa aagtcgcgcc tggcaaaggt tgcgggtgtt tcgcttgttt
    15841 tattactcgc tgcctgtagt tctgactcac gctataagcg tcaggtcagt ggtgatgaag
    15901 cctacctgga agcggcaccg cttgcggagc ttcatgcccc ggctggaatg attttgccgg
    15961 tgacctccgg tgattatgca atcccggtga ccaacggtag tggtgctgtc ggtaaggcgc
    16021 tggacattcg tccaccagcc cagccgctgg cactggtttc tggcgcgcgt acccagttca
    16081 cgggcgatac cgcttcattg ctggtggaaa atggtcgtgg caatactctg tggccgcagg
    16141 tggttagcgt gctgcaggcg aaaaactaca ccatcaccca acgtgatgat gctggtcaga
    16201 cactgaccac cgattgggta caatggaacc gtctggacga agacgagcag tatcgtggtc
    16261 gttatcaaat ctctgttaag ccgcagggtt atcagcaggc ggttacggtt aaactgttga
    16321 acctggaaca ggcgggcaaa ccggttgcag acgcggcttc catgcagcgt tacagcacgg
    16381 agatgatgaa cgttatttcc gccggtctgg ataaatctgc cactgacgcc gcgaacgctg
    16441 cgcaaaatcg tgcctccacc actatggacg tacaaagtgc agctgatgac accggtttac
    16501 caatgctggt cgtacgcggg ccgttcaatg tggtttggca acgtctgcca gcggcgctgg
    16561 aaaaagtggg catgaaagtg accgacagca cccgttcgca gggcaacatg gccgtaactt
    16621 ataagccgct gtctgacagc gactggcagg aactgggcgc gagcgatcca ggcctggcat
    16681 ccggtgacta taaactgcag gttggcgatt tagataaccg cagcagccta cagttcatcg
    16741 atccgaaagg tcatactctg actcagagtc agaacgacgc gctggtagct gtcttccagg
    16801 ctgcgtttag caagtaaaaa tacagggctg gaatcatccg gccctttttt ctgatatgat
    16861 acgcaaacgt gtgcgtcggc aggaaaacgc gattttagcg gtaattcgca cgaaatttgt
    16921 ttgtcggacg tagttcggat aaggcgttca cgccgcatcc gacaaaacat ccggcacacc
    16981 agacagcaaa agattttaaa acgttaattc acacccagga gtgataaaga tgcaaaagca
    17041 agctgagttg tatcgtggta aagcgaaaac cgtatacagc acggaaaacc cggacctgtt
    17101 ggtgctcgaa ttccgcaatg atacgtcagc aggggatggc gcgcgcattg agcagtttga
    17161 tcgcaaaggt atgttgaaca acaagttcaa ctacttcatt atgagcaaac tggctgaagc
    17221 gggtatcccg actcaaatgg agcgtctgct ctccgatacc gaatgtctgg tgaaaaagct
    17281 ggatatggtg ccggttgagt gtgtcgtgcg taaccgtgct gcgggctctc tggtgaaacg
    17341 tcttggaatc gaagaaggta ttgagctgaa cccgccgctg ttcgatctgt tcctgaaaaa
    17401 cgatgccatg cacgatccga tggtcaacga atcttactgc gaaacctttg gctgggtgag
    17461 caaagagaac ctggcgcgta tgaaagagct gacctacaaa gcgaacgacg tgctgaaaaa
    17521 actgttcgat gatgctggtc tgattctggt cgacttcaag ctggagtttg gtctgtacaa
    17581 aggtgaagtg gtactgggcg atgagttctc cccggacggc agccgcctgt gggacaaaga
    17641 aacactggag aaaatggaca aagaccgttt ccgccagagc ctcggtggcc tgatcgaagc
    17701 ctatgaagcc gtcgcccgcc gcctgggtgt acagctggac tgatttttct gttcatcatc
    17761 ttgccgtgca gctggcacgg caagacaacc gctctcggaa tatatgcatt gaacgctcat
    17821 tcctccgcca tttcatcccg ctttcctgtg gtaatcttcc tctgaaccac ctacgatcat
    17881 ttctataatg aatatattgt tgaggtatct atgcgttggc aagggcgacg tgaaagtgac
    17941 aatgtcgaag acaggcgtaa cagctccggc ggtccgtcaa tgggcggtcc cggttttcgc
    18001 ctgccaagcg gtaaaggcgg gctgatttta ctgatagtcg tgctggttgc aggctactat
    18061 ggtgttgatt taaccgggtt gatgaccgga cagccggttt cccaacagca atcaacacgg
    18121 tcaattagcc caaatgaaga cgaagccgca aaattcacct cggtgattct ggcaaccacg
    18181 gaagacacct ggggacagca attcgagaag atgggtaaaa cctatcagca accgaagctg
    18241 gtcatgtatc gtggaatgac gcgtaccggc tgcggggcgg gccagtccat tatgggaccg
    18301 ttctattgcc cggcggatgg cacggtttat atcgatctct ccttctatga tgacatgaaa
    18361 gacaaactcg gcgcggatgg cgattttgcc caggggtacg ttatcgccca tgaagtcggt
    18421 catcatgtgc agaaattgtt aggcatcgag ccgaaagttc gtcaactgca acaaaacgcg
    18481 acgcaggcgg aagtaaaccg cttatctgtg cgtatggaac tccaggccga ctgttttgcc
    18541 ggtgtctggg ggcataacat gcagcagcaa ggcgatctgg aagaggcgct gaacgcggcg
    18601 caggccatcg gcgatgaccg tttacaacag caaagtcagg ggcgagtagt accagacagt
    18661 ttcactcatg gtacttctca gcaacgctac agctggttta aacgtggttt cgacagcggc
    18721 gatccggcac aatgcaatac ttttggtaaa agcatttaac ttccggggca aggatggctg
    18781 aactgactgc gcttcacaca ttaacagcgc aaatgaaacg tgaagggatc cgccgcttgc
    18841 tggtgttgag cggggaagag gggtggtgtt ttgatcatgc gcttaagttg cgtgatgcct
    18901 tacctggcga ctggctgtgg atttcgccgc agccagatgc tgaaaaccac tgttctccct
    18961 cggcgctaca aactttactt gggcgcgagt tccggcatgc ggtattcgac gcccgccacg
    19021 gctttgatgc cgctgccttt gcggcactta gcggaacgtt gaaagcggga agctggctgg
    19081 ttttgttact ccctgtatgg gaagagtggg aaaaccaacc tgatgccgac tcgctgcgct
    19141 ggagtgattg ccctgaccct attgcgacgc cgcattttgt ccagcatctc aaacgcgtac
    19201 ttacggcgga taacgacgct attctctggc ggcaaaacca gccgttctcg ttggcgcatt
    19261 ttaccccccg tactgactgg caccccgcga ccggcgcacc acaaccagaa caacagcaac
    19321 tcttacagca gctactgacc atgccgccgg gcgtggcagc ggtaacggct gcgcgtgggc
    19381 gcggtaagtc cgcgctggca gggcaactca tttctcgtat tgcgggcagt gcgattgtca
    19441 ccgcgcccgc aaaagcggca acggatgtac tggcacaatt tgcgggcgag aagtttcgct
    19501 ttattgcgcc ggatgccttg ttagccagcg atgagcaagc cgactggctg gtggtcgatg
    19561 aagccgcagc catacctgcg ccgttgttgc atcaactggt atcgcgtttt cctcgaacgt
    19621 tgttaaccac tacggtgcag ggctacgaag gtaccggacg tggttttttg ctgaaatttt
    19681 gcgctcgctt tccgcattta caccgttttg aactgcaaca gccgatccgc tgggcacagg
    19741 gatgcccgct ggaaaaaatg gttagtgagg cactggtttt taacgatgaa aacttcaccc
    19801 atacaccaca aggcaatatc gtcatttccg catttgaaca aacgttatgg cgaagcgatc
    19861 cagaaacgcc gttaaaggtt tatcagttat tgtctggtgc gcactaccgg acctcgccgc
    19921 tggatttacg ccggatgatg gatgcaccag ggcaacattt tttacaggcg gctggcgaaa
    19981 acgagattgc cggggcgttg tggctggtgg atgagggcgg attatctcaa gaactcagtc
    20041 aggcggtatg ggcaggtttt cgtcgcccgc ggggtaatct ggtggcccag tcgctggcgg
    20101 cgcacggcag caatccactg gcggcgacat tgcgcggacg gcgggtcagc cggattgcag
    20161 tccatccggc gcgtcagcgc gaaggcgttg ggcaacagct cattgccagc gctttgcaat
    20221 ataggcctgg cctcgactat ctttcggtga gttttggtta caccggggag ttatggcgtt
    20281 tctggcaacg ctgcggtttt gtgctggtgc gaatgggtaa tcatcgtgaa gccagcagcg
    20341 gttgctatac ggcgatggca ctgttaccga tgagtgatgc gggtaaacag ctggctgaac
    20401 gtgagcatta ccgtttacgt cgcgatgcgc aagctctcgc gcagtggaat ggcgaaacgc
    20461 ttcctgttga tccactaaac aatgccgtcc tttctgacga cgactggctt gaactggccg
    20521 gttttgcttt cgctcatcgt ccgctattaa cgtcgttagg ttgcttattg cgtctgctac
    20581 aaaccagtga actggcatta ccggcgctgc gtgggcgttt acagaaaaac accagtgacg
    20641 cgcagttatg taccacactt aaactttcag gccgcaagat gttactggtt cgccagcggg
    20701 aagaggccgc gcaggcgctg ttcgcactta atgaggttcg cactgagcgt ctgcgcgatc
    20761 gcataacgca atggcaattt tttcactgac tccttcaatt ttctcccatg gtcattgtga
    20821 gtcagcggcg taaaattaat gacatcagtt aaggagttcg ccatgaaaca tgaccatttt
    20881 gttgttcaaa gcccggataa acctgcgcaa cagttgttac tgcttttcca cggtgtcggg
    20941 gataacccgg tggcaatggg ggaaataggc agctggtttg cgccgctatt tcctgatgcg
    21001 ctggtggtga gtgtcggagg cgcggagccg agcggtaatc cggcggggcg tcagtggttt
    21061 tcggtgcagg gtattacgga agataatcgc caggcgcgtg tggatgccat catgccgacg
    21121 tttattgaga tggtgcgcta ctggcagaaa cagagcggag taggggctaa cgccacagcg
    21181 ctaatcggtt tctctcaagg cgcgattatg gcgctggaga gcattaaagc cgaaccgggc
    21241 cttgcttcac gcgtcattgc ttttaacgga cgctatgcga gcctgccgga aactgcgtct
    21301 acagccacga caattcacct gattcatggc ggtgaagatc cggttatcga tctggcgcat
    21361 gcggttgcgg cgcaggaagc gttaatcagt gccggtggtg acgtgacgct ggatatcgtg
    21421 gaggatttag gccatgcgat cgacaatcgc agtatgcaat ttgccctgga tcacttgcgt
    21481 tataccattc cgaaacatta tttcgacgaa gcgctaagtg gcggtaagcc tggcgatgat
    21541 gatgtgattg aaatgatgta aaagacagat tacctggcct gataagacgc gtgagcgtcg
    21601 catcaggcat cgttcaccat tgccggatgc ggcgttaacg ccttatccgg cctacggttg
    21661 agcactcagc ttacttcttc ggttgatcct ttttcggcca gtcgtcgtca tcgtcccact
    21721 tatcgttgaa atcacgatgc gggggaagct ccggcttgtt ggcgagaaat ttcttgtggt
    21781 cgacgcgctt gagatcttta atcacgttca gcaggacgcc taccaaaaag acaatcacca
    21841 gaatccacca atatttagcc agccagtcca tgcttatttc ctcttgcaga accactcatc
    21901 aggcgacgag ctgttccatg atacgttgat acatacgggc aagtagctgc aggtcggcag
    21961 cgttcacaca ttcattaatt ttatgaatag tggcattgac cggcccgagt tccaccacct
    22021 gcgcccccat gcgagcaata aagcgcccgt cggacgttcc gcctgtggtc agtagctgcg
    22081 gtttaatttc attatagtgc tcaaccgcgt taacgaccgc atccaccagt ttaccgcgcg
    22141 cggtcaaaaa tggctgcccg gaaagccacc aatccaccgt atagcgcagt tgatgttttt
    22201 caagcagggc aagcacctgc gctttgatca tctcatcagt cagttcggtg ctgaagcgga
    22261 agttaaactg cacaaacagt tcacccggaa taacgttgtt actgcccgtt cccgcctgaa
    22321 tattggcaat ctgcatactg gtcgccggga agaattcatt gccctgatcc cactcaatag
    22381 ccactaattc attaaggaaa ggtgctgcgc gatgtactgg attgtcagcc agatgtgggt
    22441 aggccacatg cccctgcacg ccatgaatgg taaggttgca ggttaacgat ccgcgacgac
    22501 catttttcac cacatcacca accacttcga tactcgacgg ttcgccgacc agacagtaat
    22561 cgagacgctc attacgcgcc attaacgctt cgacgacttt taccgtaccg ttgtgggcac
    22621 tggcttcttc atcagaggtg atcagaaatg ccagtcgtcc cgtatggttg ggatgttgtg
    22681 cgacaaaacg ttctgctgct accaccatcg ccgccagcga gcctttcata tctgccgcac
    22741 cgcgcccgaa taacatgcca tcacgaatgg tgggttcaaa cggcggattg atccaacgat
    22801 cggcgtcgcc aggcggcacc acgtcggtat gcccggcaaa ggccaatgtt tcaccttgcc
    22861 cacgccatgc ccaaaaattc tgcgtatcgg caaagtccat gcgttcaacg gtaaaaccga
    22921 tcgcctgcaa acgatcaatc aacaaagcct ggcatcctgc gtcatcagga ctcagggaag
    22981 ggcggcgaat aagctgttgt gtcagctcaa taaccgggca cgacatagac tacacctcat
    23041 ggaaaaattg ctgataactg gaatcactga aacccagcag cataggctta ccgggcgcgc
    23101 agagcaatgg acgtttgata attgcaggca tttcagtcat taatgccgcc gctgaggccg
    23161 catcggtgat tttattgcgg gtggtttcgt ccagtttacg ccaggttgta ccacgggtgt
    23221 tgagtaacgc ctcccaacct aattcgttga taaaaccgtt caataattcg ctgtccagcc
    23281 catcgacgcg gtaatcatga aaacgatagt cgatgttatt ggcttccagc caacggcgag
    23341 cctttttaat ggtgtcacaa tttttgatac cgtaaagtgt aaccataatg aatccttttt
    23401 cgatttatga gaataaatac tcatttaagg gtgaaaacat tttgcctgca aatgattatc
    23461 aggaaatata acattatcga agtattcaa
//
LOCUS       DADMKF010000054        23235 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.055, whole genome shotgun sequence.
ACCESSION   DADMKF010000054 DADMKF010000000
VERSION     DADMKF010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23235
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.055"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(38..238)
                     /gene="glgS"
                     /locus_tag="JNC24_001968"
     CDS             complement(38..238)
                     /gene="glgS"
                     /locus_tag="JNC24_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HAY9158717.1"
                     /translation="MDHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQARELELEH"
     gene            507..1136
                     /locus_tag="JNC24_001969"
     CDS             507..1136
                     /locus_tag="JNC24_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqiJ family protein"
                     /protein_id="HAY9158718.1"
                     /translation="MILFADYNTPYLFAISFVLLIGLLEILALICGHMLSGALDAHLD
                     HYNSITTGHISQALHYLNIGRLPALVVLCLLAGFFGLIGILLQHACIMVWQSPLSNLF
                     VVPVSLLFTIIAVHYTGKIVAPWIPRDHSSAITEEEYIGSMALITGHQATSGNPCEGK
                     LTDQFGQIHYLLLEPEEGKIFTKGVKVLIICRLSATRYLAENNPWPQIL"
     gene            1163..2824
                     /locus_tag="JNC24_001970"
     CDS             1163..2824
                     /locus_tag="JNC24_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HAY9158719.1"
                     /translation="MDDIVNSVPSWMFTAIIAVCILFIIGIIFARLYRRASAEQAFVR
                     TGLGGQKVVMSGGAIVMPIFHEIIPINMNTLKLEVSRSTIDSLITKDRMRVDVVVAFF
                     VRVKPSVEGIATAAQTLGQRTLSPEDLRMLVEDKFVDALRATAAQMTMHELQDTRENF
                     VQGVQNTVAEDLSKNGLELESVSLTNFNQTSKEHFNPNNAFDAEGLTKLTQETERRRR
                     ERNEVEQDVEVAVREKNRDALSRKLEIEQQEAFMTLEQEQQVKTRTAEQNARIAAFEA
                     ERRREAEQTRILAERQIQETEIEREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKS
                     IAIAAKSEQQSQAEARANLALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQDAETK
                     AVELTVRAKAEKEAAEMQAAAIVELAEATRKKGLAEAEAQRALNDAINVLSDEQTSLK
                     FKLALLQALPAVIEKSVEPMKSIDGIKIIQVDGLNRGSAAGDANTGNVGGGNLAEQAL
                     SAALSYRTQAPLIDSLLNEIGVSGGSLAALTSPLTSTTPVAENVE"
     gene            3063..3122
                     /locus_tag="JNC24_001971"
     CDS             3063..3122
                     /locus_tag="JNC24_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HAY9158720.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            3439..3498
                     /gene="ibsE"
                     /locus_tag="JNC24_001972"
     CDS             3439..3498
                     /gene="ibsE"
                     /locus_tag="JNC24_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsE"
                     /protein_id="HAY9158721.1"
                     /translation="MMKFVIILVVLLLLSFPTY"
     gene            complement(3618..5051)
                     /gene="hldE"
                     /locus_tag="JNC24_001973"
     CDS             complement(3618..5051)
                     /gene="hldE"
                     /locus_tag="JNC24_001973"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HAY9158722.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPSSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVSYLANARKQGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(5099..7939)
                     /gene="glnE"
                     /locus_tag="JNC24_001974"
     CDS             complement(5099..7939)
                     /gene="glnE"
                     /locus_tag="JNC24_001974"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HAY9158723.1"
                     /translation="MKPLSSPLQQYRQTVVERLPEPLAEEPLSVQAKSVLTFSDFVQD
                     SIIAHREWLTELESQPPQADEWQHYAAWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSYLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPSLQSCSLLPTLSAIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMNNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTLISDFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALNHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWVQMVARYGKPNHLNDREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSTEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGRTLQTEVREMREKMRAHLGNKHRDRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSGDCFTAERDLVRASWQKWLVEE"
     gene            complement(7962..9263)
                     /locus_tag="JNC24_001975"
     CDS             complement(7962..9263)
                     /locus_tag="JNC24_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HAY9158724.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRRHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQFPSEV
                     WPNGELPADLASRVQPLFSTDFYREKWLVAVDGSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELALAQWQYHEELWVRGNDAAKEQVLAAISLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQGKI
                     SDSFKRFADIHLSHHAAELKSVFCQPLGDLYRDQLPRLTRDIDSILLLAGYYDPVVAH
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            9505..10125
                     /locus_tag="JNC24_001976"
     CDS             9505..10125
                     /locus_tag="JNC24_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HAY9158725.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            10189..11427
                     /gene="cca"
                     /locus_tag="JNC24_001977"
     CDS             10189..11427
                     /gene="cca"
                     /locus_tag="JNC24_001977"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HAY9158726.1"
                     /translation="MKIYLVGGAIRDALLGLPVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTDFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREAWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
     gene            complement(11590..12411)
                     /gene="bacA"
                     /locus_tag="JNC24_001978"
     CDS             complement(11590..12411)
                     /gene="bacA"
                     /locus_tag="JNC24_001978"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HAY9158727.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDISMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(12502..12870)
                     /gene="folB"
                     /locus_tag="JNC24_001979"
     CDS             complement(12502..12870)
                     /gene="folB"
                     /locus_tag="JNC24_001979"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HAY9158728.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAM
                     ARAANVGVIIERGNNLKENN"
     gene            12975..13592
                     /gene="plsY"
                     /locus_tag="JNC24_001980"
     CDS             12975..13592
                     /gene="plsY"
                     /locus_tag="JNC24_001980"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HAY9158729.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(13605..14537)
                     /gene="ttdR"
                     /locus_tag="JNC24_001981"
                     /gene_synonym="dan"
                     /pseudo
     CDS             complement(13605..14537)
                     /gene="ttdR"
                     /locus_tag="JNC24_001981"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TtdR"
     gene            14744..15655
                     /gene="ttdA"
                     /locus_tag="JNC24_001982"
     CDS             14744..15655
                     /gene="ttdA"
                     /locus_tag="JNC24_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
                     /protein_id="HAY9158730.1"
                     /translation="MMSESNKQQAVNKLTEIVANFTAMISTRMPDDVVDKLKQLKDAE
                     TSSMGKIIYHTMFDNMQKAIDLNRPACQDTGEIMFFVKVGSRFPLLGELQSILKQAVE
                     EATVKAPLRHNAVEIFDEVNTGKNTGSGVPWVTWDIIPDNDDAEIEVYMAGGGCTLPG
                     RSKVLMPSEGYEGVVKFVFENISTLAVNACPPVLVGVGIATSVETAAVLSRKAILRPI
                     GSRHPNPKAAELELRLEEGLNRLGIGPQGLTGNSSVMGVHIESAARHPSTIGVAVSTG
                     CWAHRRGTLLVHADLTFENLSHTRSAL"
     gene            15652..16257
                     /gene="ttdB"
                     /locus_tag="JNC24_001983"
     CDS             15652..16257
                     /gene="ttdB"
                     /locus_tag="JNC24_001983"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HAY9158731.1"
                     /translation="MKKILTTPIKAEDLQDIRVDDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            16306..17769
                     /locus_tag="JNC24_001984"
     CDS             16306..17769
                     /locus_tag="JNC24_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HAY9158732.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALIPVPAGLESHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLIVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVALIVVFYLLRY
                     FFASATAYTSALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(17812..18825)
                     /gene="tsaD"
                     /locus_tag="JNC24_001985"
     CDS             complement(17812..18825)
                     /gene="tsaD"
                     /locus_tag="JNC24_001985"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HAY9158733.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            19062..19277
                     /gene="rpsU"
                     /locus_tag="JNC24_001986"
     CDS             19062..19277
                     /gene="rpsU"
                     /locus_tag="JNC24_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HAY9158734.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            19388..21133
                     /gene="dnaG"
                     /locus_tag="JNC24_001987"
     CDS             19388..21133
                     /gene="dnaG"
                     /locus_tag="JNC24_001987"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HAY9158735.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            21328..23169
                     /gene="rpoD"
                     /locus_tag="JNC24_001988"
     CDS             21328..23169
                     /gene="rpoD"
                     /locus_tag="JNC24_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HAY9158736.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
BASE COUNT         5654 a         6331 c         5905 g         5345 t
ORIGIN      
        1 aatacaaaca ctaaaacggg ccatcaggcc cgttatatca gtgctctaac tccagctctc
       61 ttgcctgcat catctgttgg caataccagg cataacgtgc gcaaaaccag gttctatgtt
      121 cttcatccat gttaccagta acggcaagaa tatcgaccgg gcgaccttct gcgtgcattc
      181 tggcaaaact acgcgccagg aaatcgaaat tatttaaaga attaagacta tgatccataa
      241 gcacatcctc cggttatcgc atacctttta aggccatatg ctttttctga tttcagtatt
      301 ccccaatcag tgtagtgcct ttaaacataa cgtgcgtaaa tatattgtcc ccgatcacac
      361 tttttagtga aaaataaaaa actatattaa aaaactttct cttccagaac ttctctaaca
      421 tgtgttatta ttaacaaaga taaatgtaag caaggaaact atcccaaagt aaatattgcg
      481 tcactaaatg gacattggag tgacatatga ttttattcgc cgactataat accccttatc
      541 ttttcgctat ttcctttgta ctgttgatcg gccttttaga aattttagcg cttatctgcg
      601 gtcacatgct ctccggtgct cttgatgcac atcttgacca ttataattcc ataaccacag
      661 gtcatattag ccaggcactt cattatctca atatcggaag attgccagct ctcgtcgttc
      721 tctgcttgct ggctggtttt tttggtctta ttggcatcct gttgcagcat gcctgcatca
      781 tggtctggca atcaccactg tcgaacctgt tcgtggttcc tgtcagttta ctgtttacga
      841 ttattgcggt gcattatacc ggtaaaatag ttgcgccctg gattccacgc gaccatagtt
      901 cagctattac agaagaagaa tatattggta gcatggcatt aattaccggt catcaagcca
      961 cttcaggtaa tccctgcgaa ggaaaactca ccgatcaatt tggccaaata cattatttac
     1021 tactggagcc tgaagaagga aaaattttca ctaaaggagt caaagtgtta attatttgcc
     1081 gactttctgc aacgcgatat ttggcggaaa acaatccctg gccacaaata ttgtaattta
     1141 ttaatcaaaa ggaaatttaa taatggatga tattgttaat tctgtgccct cctggatgtt
     1201 taccgcgatt attgccgtat gcattctgtt tattattgga attattttcg ccaggctcta
     1261 tcgtcgcgct tcggcagagc aagcttttgt tcgtactggt ttaggtgggc aaaaagtggt
     1321 gatgagcggt ggcgcaatcg tgatgccgat ctttcatgaa ataatcccca tcaatatgaa
     1381 tactctgaag ctggaagtca gccgctcaac cattgatagc ctgattacga aagatcgtat
     1441 gcgtgtcgat gtagtagtcg ctttctttgt acgggtaaaa ccgtctgtag aaggcatcgc
     1501 cactgcggcg cagacgttgg ggcaacgcac gctatcacca gaagacttac gtatgttggt
     1561 tgaagataaa tttgtcgatg ccctccgtgc aacagctgca caaatgacca tgcatgagtt
     1621 acaggatacc cgcgagaact tcgtgcaggg agtacaaaat actgtcgcag aagacctatc
     1681 gaaaaacggt cttgagctgg aaagcgtttc acttaccaac tttaaccaga cctcgaaaga
     1741 acatttcaat ccgaacaatg cctttgacgc cgaaggttta accaaactga ctcaggagac
     1801 ggagcgccgt cgtcgcgaac gtaacgaagt tgaacaggat gtagaagttg cggtgcgtga
     1861 gaaaaaccgt gatgcacttt cgcgcaagtt ggagattgaa caacaagaag cgtttatgac
     1921 gcttgagcag gagcagcagg ttaaaacccg tactgccgaa cagaatgcac gtattgcggc
     1981 ttttgaagct gaacgtcgtc gtgaagcaga gcagacgcga attctggctg aacgacaaat
     2041 tcaggaaacg gagatcgagc gcgagcaggc cgtccgctca agaaaggttg aggctgaacg
     2101 tgaagttcgt attaaagaga tcgaacagca gcaggtcacc gaaatcgcca accagacgaa
     2161 atcgatcgct attgccgcca aatcggaaca acagtcccag gcagaagcgc gtgctaacct
     2221 tgcactcgca gaagcagtaa gtgcgcaaca aaacgtagaa accactcgcc agaccgcaga
     2281 agccgatcgt gctaaacaag ttgccctaat cgctgccgcg caggatgcag aaaccaaagc
     2341 ggttgaactg accgtgcggg cgaaagcaga aaaagaagcc gcagagatgc aggcggcggc
     2401 tatcgttgag ttagccgaag ctacacgtaa aaagggtctg gcggaagcag aagcacaacg
     2461 tgcgctgaac gatgctatca acgtactttc tgatgaacaa accagcctta aattcaaact
     2521 ggccttgttg caggcgctac ctgcggtaat agagaaatcc gttgagccga tgaagtccat
     2581 tgacggtatc aagattattc aggtcgatgg tctgaatcgt ggcagcgctg cgggtgatgc
     2641 aaacacgggt aatgtggggg gcggaaacct agcggaacaa gcattatcag ccgctctctc
     2701 ttaccgcaca caggcaccgc tgattgactc cttgctcaat gaaattggcg tttcaggcgg
     2761 ctcactggcg gcattgactt cacccttaac ctcaacaact cccgtcgccg aaaacgtaga
     2821 ataaattttc agctatgaac gtccctggca caaaaagccg gggatttttt atatctgcgt
     2881 tccgctaaaa actgcaaatg atcaggccgt tgcaacgttt tgcgtgaccg ctcggggaag
     2941 gcaaaactgc ctctgggaaa gcgttgcgcg aggtccggcg ctcatcaaca atcggggagt
     3001 cagcaaaggg ctgaaacggg aaagcccctc ccgaggaagg ggccttacag aaggaaaggg
     3061 ttatgatgaa gctcgtcatc atactgattg tgttgttact cgtaagtttc gcagcttatt
     3121 aacagccaat cagagggggg agaaatcctc cctcaccctt gttcctttac tttaggttga
     3181 aaaaaaatca gcgtcaagaa ggtttcatta acacatatcc ctggcccaaa aagccgggga
     3241 ttttttatat ctgcgttccg ctaaaaggtg caaatgctca ggacgttgca gcgttttgcg
     3301 tgatcgctcg gggaaggcaa aactgcttca gggaaagcgt tgcgcggggt ccggcgctca
     3361 tcaacaatcg gggagtcagc aaggggctga aacgggaaag cccctcccga ggaaggggcc
     3421 ttatagaagg aaagggttat gatgaagttc gtcatcatac tggttgtgtt gttactgtta
     3481 agtttcccga cttactaaca actgatcaga gggggaagaa atcctccctt acccttgttc
     3541 ctttacttta ggttgaaaaa acaacagcgt caataggcct gcctgctaca aagcgagatc
     3601 tgtgaaccgc tttccggtta gcctttttta tcctgttgga tcttcttgat gatgttagtg
     3661 gtcgagcaac cgtcttcgaa gttgagcacc agcacttcgc cgccgttggc ccagacttct
     3721 ttactcccgg cgatctcttc tggtttatag tcgccgccct tcaccagtaa atctggcagg
     3781 atcccggcga tcaagcgctg cggcgtgtct tcttcaaacg acaccaccca gtcgaccgct
     3841 tccagtgcgc ccagcacaat catacgctgt tcgagcgggt taacggggcg ggaatcccct
     3901 ttcagccgtt tggtggaggc atcgctgttc acagcgacaa tcagacggtc accctgtttg
     3961 cgagcatttg ccagatagga gacgtgcccg gcgtgcagga tgtcaaagac accgttggtc
     4021 attaccacct tttcgccgcg tttacgcgct gccgctacgg ccagcttcag ttcctcttcg
     4081 gtcatcacgc caaagccggt atctgcgcgc ccacgcactg cattttccag ctcgatcggc
     4141 gaaaccgtgg aggttcccag tttgccgacc accacgccag ccgccgcatt ggcaaagaag
     4201 caggcttctt ccagcgaatt acccgctgcc agcgttgccg ccaggacgcc aatcaccgtg
     4261 tcgcccgcac cggtaacgtc atacacttcc tgcgcctggg ttggcatatg cagcggcgct
     4321 ttacccggtt gcagcagcga cataccctgt tcggaacggg tcactaacag agccgagagt
     4381 tcgtaatcgg caatcagttt catgccgcgc tcaacaatct cttcttcggt cttacattta
     4441 ccgacaacag cttcaaattc cgagagattc ggcgttaaca gcgtagcgcc gcggtagcgc
     4501 tcaaaatcgg taccttttgg atcaatcagc accggaacgc ccgctttacg cgccagttgg
     4561 atcatctgct gtacgcttgc cagcgcacct ttggcgtagt cagaaagcac cagcgcgcca
     4621 atcgaactca gcgcctgatt aatccgctcg tgcagcggct gcggatcaac gccttcgaaa
     4681 ccttcttcga aatccagacg gatcagctgt tggttgcggg aaagtacccg taatttggtg
     4741 atggtcggat gcgtcggtac agaaacgaag tcgcatttga cgttgacgtc ggccagagat
     4801 ttactcagcg cgcgcgctgc atcgtcaatg cccgtcaacc cgaccaggcg tgcattagca
     4861 ccgagagaag cgatattcat cgccacgtta gccgcgccgc ccggacgttc ttcgatggta
     4921 ttcactttaa ccacgggcac cggcgcttcc ggcgagatac ggctggaggg accgtaccag
     4981 taacgatcca gcattacatc accaaccacc atcactcctg cacgttcaaa ctctggcagc
     5041 gttactttca ttcctgtctc ctgagagatt caaaatttgc gcgcgataat accatacttc
     5101 attcttccac cagccacttc tgccagcttg cccgcaccag atcacgctct gcggtgaagc
     5161 aatcccccga cacatggccc ggcaattcct gtaatgccag atgatgaagt tcatcgcgca
     5221 gcgtcgtgta agcacgggtc agcgccatcg cttcctgctc ttccataatg tcgttttgcg
     5281 ccagcagctc cagaatacgc acgttgtctg accagcgcgt taacttcggt ttttcatgag
     5341 cgtagcgcaa caccagatat tgggtaataa attcgatatc ggtaattccg ccttcatcag
     5401 ctttgatatc aaagcgatcg cgatgtttat tgccgagatg agcgcgcatt ttctcgcgca
     5461 tttcccgcac ttccgtttgc agagttctac cttcacgcgg cagcgtcata atctcgcgac
     5521 gcactgcgtc aaagtgcgcg gtgagctgcg gatcgccgta cactacccgc gcacgcacca
     5581 gcgcctgatg ttcccacgtc caggcctcgt ttttctgata atcggcaaat gcttctgtgg
     5641 aggtaaccag cattcccgcc gccccggacg ggcgcagccg cgcatccact tcgtacaaaa
     5701 tgccggaaga ggttcgcgtg ctgaacaggt gcataatgcg ttgcgccaga cgaagataaa
     5761 actgtcgtcc gtcgatttcc cgctcgccgt cagtcatcgc atccattggg caatcatgga
     5821 ggaagatcag gtcaagatcg gaactgtagc ctaactccca gccgcccagc ttgccgtagc
     5881 cgaccactgc aaaaccacgc ccttcgcgat cgttgaggtg attcggctta ccgtagcggg
     5941 caaccatttg aacccacgcc tgctgaacga cggcatctat catggcttcc gccagccagg
     6001 ttaagtgatc gctcactttc atcaccggta gcgtaccagc gatatccgct gcggcgatgc
     6061 gtaacagctg cgcctgtttg aactgacgca gcgcctcaag ctgttgctct tcgtcatctt
     6121 ccggcacgcg cagcaaatac tggcgcaact catcgcggta ggcatcggtc gccgtcggct
     6181 ggtaaagagt gtttggatcg agcaattcat ccagcaataa tggataacgc gccagctggc
     6241 tggcaatcat cggcgatgcg gcacacagag aaatcaaatg gttaagcgcc gcagggaatt
     6301 cactgagcaa ttctaaatag gtggtgcggg taacaatccc caccagcaag gcggtaatgc
     6361 gcgacagcgt gacagcagcg tcttcacgcg cacagacatc acttagcaga tgcggcatca
     6421 gatggtcgag cacctgacgt cctcgcggcc cgatggtgcg cttatccagc tctttgcgga
     6481 aatcggaaat cagcgttagc acctgtttgc gatcatcctc gctaagatgc gccagcactg
     6541 gcgtagtgtc atcttcctgc aacgcatcct gccacagctc acgccactgt tccgacagcg
     6601 actcttcctg agtttcactt tcgtcgtcgc caatcaattc attaaacacc cggcgcacat
     6661 tgttcatatg tgcggtcagc gccccggtca gttgcggcca gtcagcaaag tccatcgccc
     6721 acgccagccg cgcacggtta agctcatcag aaggaagcgt ctgggtttgt tcgtcgttaa
     6781 tgctttgcag caggttttcc agacgccgca ggaacagata cgccactcgc aattgttcag
     6841 catcgttttc agaaagcaga tgcagcgcag caatggcgct gagcgttggc agtaaagagc
     6901 acgattgcag cgacggttcg cgcccgccgc gaatgagctg gaatacctgg acgataaatt
     6961 caatttcgcg aataccaccc gcgccaagtt tgatgttgtc ggtcaaaccg cgccgacgca
     7021 cttcacgggc aatcattcct ttcatgttgc gcagcgactg aatcacgctg aaatcgatgt
     7081 aacgacggaa aacaaacggg cgtagcatcg cacgcaactc gttagcatag acgccttcgc
     7141 tatcgcccat aatccgcgct ttgaccatcg cgtaacgctc ccagtcgcgc ccctgctcct
     7201 ggtaataatc ctccaacgcg gcaaagctca gcaccagcgg gccactttcg ccaaacggac
     7261 gcagtcgcat atccacgcga tagacgaagc catcctgcgt tggttgatcc agcactttaa
     7321 tcagccgctg ccccatgcgg gtaaaaaact gcgcgttatc cagttcccgg cgtccgccct
     7381 gcgtacaacc atgttccggc caggcaaaaa tcagatcgat atcagaggag aaattcagtt
     7441 ccccgccgcc cagcttaccc atgcctaaga tcagcagcgg ttgcgcttcg ccctgcgcat
     7501 tgcacggcgt tccccactcg cggcagcagg cgtcatacag ccagtcacgc gccgcaacaa
     7561 tcagcgtctc cgccagataa ctgagctgct gcaatatgct ctcttcagta accagtgcca
     7621 gcgtttgcgc ccatgcgatg cgcaccataa tgcgccgccg gaatagccgc agctcgcgca
     7681 ttaacccggc ttcgtcactc acattactga gcgcctcctg caaccatgca gcgtaatgct
     7741 gccattcatc ggcctgcggc ggttggcttt ccagttccgt caaccactct cgatgcgcaa
     7801 ttatgctgtc ctgcacaaaa tcactgaaag taagtactga cttcgcctgt acacttagtg
     7861 gttcctcggc taaaggctct ggcagccgct caacaacggt ctgcctgtac tgctgtaacg
     7921 gtgaagagag cggcttcata agcgatttta tccttgcctg attaacgttt tccgctgtgc
     7981 aaccagaacg gttcctgatt gtttgcctca ttacggaaat gctcaatttc aatgcgttgc
     8041 ccggtcgcaa tagcgtgacg cagcccctgc cagttctcca gccaggcatg cgcgacgaca
     8101 ggatcatagt aacccgccag caacagtatt gagtcaatgt cacgcgtcag gcgtggcaac
     8161 tggtcacggt agagatcgcc taacggctgg cagaaaacgc ttttcagttc agcggcatgg
     8221 tgggaaagat ggatatcggc aaagcgtttg aaggagtcgc tgattttgcc ctgcgctttg
     8281 gcatctaaaa atggctgcca tgctttgctt accaaccatt cggtcaacgc cagcttcgcc
     8341 attgccgttt cggtagagta gaccgccgtc acggcagaaa ccgcagaagc aatggtcgct
     8401 tcgcattggg tcagcaaatc acgtaagtga gtgctcgctt tacgcggcac aataccaccg
     8461 aacagcatca gcgtatgacg gaccaggcta atggctgcca gcacctgttc tttcgccgca
     8521 tcgttaccgc gtacccacag ttcttcatga tactgccatt gcgctaacgc cagctcgagc
     8581 gccgcttcca gcccctgttc tacatcggct tttgccgcaa catgcaaaat ggtggtcggt
     8641 ttgatttcac gcgccggatt gccctgcgcc agatgatagc cacgcgccgc tttgctcagg
     8701 ctgccctggc gtaatccggt ttgcgatacc agttggttcg ccagtttcag caccgcgcgc
     8761 gtgtcgccgc taagcagttc cagttccagc tcacagatag gttcagcaaa ttcccccgct
     8821 ttcacctccc cctgatcgag agcgatttca attcggctgc catcgaccgc caccagccat
     8881 ttttcgcgat aaaaatcggt gctgaacagc ggttgtacgc gggaagcgag atcggcaggc
     8941 aattcaccgt tcggccagac ttccgaggga aactgcgcca ggtcgagcgt cggttcgctc
     9001 aacgccacgt tatattccgg gcgctggtgt aagccgcctg tcactcttcc tgcaactttc
     9061 atggtcatct catagcgacc gttttcgcca cgaatacgta agcccatatc gtgcctacgc
     9121 agccagttat ccggcgtttc gtagtaaata ttcagcaact gcacggggtc atggtgctcg
     9181 ccgcccagcg tattgagatg gtcacgcaac gcctcaacgg cactgtgatt aacaataaac
     9241 tttaattcga tttcctgagc catggccttt acttatcggt tatgtcacat aagtgacgat
     9301 gaacggcgaa cttaatgcga tcttttttgt cagtagatag tattttgcgc caaattgcca
     9361 tgcaacgggc aatttgacgg gcgtaaaagt ttgaagcagt ggcaagacct gacacagagg
     9421 attccgattg atgtcgaatg ctttgcgtag cgacactgat gccactacta tcgttccact
     9481 ttccatgaca ataacgacag cctgatgcca aaattacgcc tgatcggatt aactttactc
     9541 gcacttagcg cgactgccgt ctcacacgct gaagaaacgc gctatgtttc tgacgaactg
     9601 aatacctggg tccgtagcgg tccgggagat cattatcgcc tcgtgggcac ggttaacgcc
     9661 ggcgaggaag tgaccttatt acaaactgac gccaacacca attatgccca ggtgaaagac
     9721 agctctggcc gtaccgcctg gatcccgttg aaacaactta gcactgagcc aagcctgcgc
     9781 tcccgtgtgc cagatctgga aaatcaggtc aaaaccctga ccgataaact caccaatatc
     9841 gataacacct ggaatcagcg caccgcagaa atgcagcaaa aagtggcgca gagcgacagc
     9901 gtgatcaacg ggttaaaaga agaaaatcag aaactgaaaa acgagctgat tgtcgcgcag
     9961 aaaaaggtcg atgccgccag cgtacagctg gatgacaaac agcgcaccat catcatgcaa
    10021 tggtttatgt atggcggtgg cgtgctgggg cttggcttgc tgctcggtct ggtactgccg
    10081 cacctgatcc caagccgcaa acgcaaagat cgctggatga actaaatcgc cttcttcgtt
    10141 gcactgtctt attattagga attgtgtatt tgacgaaggg atgatggcgt gaagatttat
    10201 ctggtcggtg gtgctattcg ggatgcattg ttagggctac cggtcaaaga cagagattgg
    10261 gtggtagtcg gcagtacgcc acaggagatg ctcgacgcgg gctaccagca ggtaggccgc
    10321 gattttcctg tgtttctaca tccgcaaacg catgaagagt atgcgctggc acgtaccgaa
    10381 cggaaatccg gttccggtta caccgatttt acttgctatg ccgcaccgga tgtcacgctg
    10441 gaagatgatc ttaagcgtcg cgatctgacc attaatgcgc tggcccagga cgataacggc
    10501 gagattatcg acccgtacaa cggtctgggc gatctgcaaa atcgtctgtt gcgccatgtt
    10561 tcccccgctt ttggcgaaga tccgttacgc gtactgcgcg tggcgcgttt tgctgcgcgt
    10621 tatgcccacc tcggttttcg tattgccgat gaaactctga cgctgatgcg cgagatgacc
    10681 catgcgggtg aactggaaca cctgacgcct gaacgggtat ggaaagagac ggaaagcgcc
    10741 ctcaccaccc gcaatccaca ggtgttcttc caggtactgc gcgattgcgg cgcactgcgc
    10801 gttttattcc cggaaattga cgcactgttt ggcgttccgg cccctgccag gtggcatccg
    10861 gaaatcgaca cgggtattca taccttaatg acgctctcaa tggcggcgat gctgagtccg
    10921 caggtcgatg tccgtttcgc aactttatgc cacgatctcg gtaaaggact gacgccgcca
    10981 gaactttggc cgcgtcatca tggtcacggt ccggcgggtg tgaaattagt ggaacaactc
    11041 tgccagcgtc tgcgcgtgcc aaatgaaatt cgcgatttag ccagactggt ggctgagttt
    11101 cacgatctca tccacacctt cccaatgctg aacccaaaaa ctatcgtcaa attgtttgat
    11161 tccatcgacg cctggcgtaa accgcagcgt gtcgagcaac tggcgctgac cagcgaggct
    11221 gacgtacgcg gcagaaccgg ttttgaatca gcggactacc cgcaaggccg ttggttgcgc
    11281 gaagcctggg aagtggcgca gtcagtgccg acaaaagcgg tcgttgaagc gggatttaaa
    11341 ggtgtggaga ttcgcgagga gctgacccga cggcggattg cggcggtagc cagttggaag
    11401 gaacaacgtt gcccaaagcc tgactgagta taaaactaaa cgttaacacg aaccatcccc
    11461 tcgccccttc ggggagaggg ttagggtgag gggaacagac cagcaccggt gcgaacaatc
    11521 acccccaccc cggccctctc cctacagagc gagggagaaa accaggcctg ctcccgaacc
    11581 ttttctcaat caaaagaaca cgacgtacac cgcagccgcc acaataaagc gataaatggc
    11641 gaacgggata aacgaaatgc gcttaatcaa ttgcaggaag gttttaatcg ctatcagcgc
    11701 caccacaaaa gcggtgataa acccaacggc aaacatcgag atatcgccgg ttgtcaggaa
    11761 gccccagctt ttgtagagat cgagcgccgt tgcgcccatc atcatcggta ccgccagcag
    11821 gaacgagaac tcggaagcag cgtaacggct cacgcccatc agcatcccac ctgaaatggt
    11881 ggccccggaa cgggagaaac ccggccacag cgccagacac tggaaacagc caatcataaa
    11941 cgcctgacga taggtcatat catcaagacc cggcgcacgc ggctctttcg gcttcaggca
    12001 ttcggcggca atcagcaaca aaccgccaac gaccagtgca tacatcacat ttatcgggtt
    12061 aaacaatgac ttaatcgtgt cgtggaacaa cagccccagt accaccgccg gaatcatccc
    12121 cagcaaaatg tggatcagcg ttaaacgacc tttgctttct ccttcgtgct gcaacgggcg
    12181 gccaaagtgg atgccaatca ggccaaacag acgccgccag aacatcacta ctaccgccag
    12241 aattgatcct aactggatca caacttcaaa ggttttcgcc gtgtcgccct cgaaccccaa
    12301 caagtgaccg acaataatca tatggcccgt gctggatacc ggcagaaatt ctgtcaatcc
    12361 ttcgaccaca cccaatattg ccgctatcag cagcgagtgc atatcgctca tcaataaacc
    12421 cctaaattat taaaatgtac cgcttgtccg gactactgca gtaagaccag gttataaccg
    12481 tttggtttaa cagctgtaaa attaattatt ttctttcaga ttattgccac gctcaatgat
    12541 tacaccaaca ttcgccgccc gcgccattgc gcctggcttg ctgagtttga tgcgcaccca
    12601 cggcgagttg aagcgtgcta acagcagctc cgccacctct tcagccacgc gttccaccag
    12661 cgcaaaacgc gccccctcga cgtggctgac caccgtttct gcaatgtcag cgtaactgag
    12721 gcaatccgcc acatcatcac ttttcgccgc tttacggtta tcccatgcca tttcgatatc
    12781 gaacactaac ttctgttcga tggtctgttc ccagtcgtaa acaccaatag tggtgattac
    12841 cgaaagttgc tctataaata caatatccat cacgtcctgc ctgctttttg gctaaccgga
    12901 taccacttcc ggcgaaatgt gcgtattatc cacagattca tcgttgaaca cgaattttca
    12961 aaacggaaca gcttatgagt gcaatcgcgc ctggaatgat cctcatcgcg tacctctgcg
    13021 gctccatttc cagtgccatt ctggtttgcc gcttgtgtgg gctgcccgat ccgcgaacca
    13081 gcggctccgg caatccaggc gcaaccaatg tgttacgtat cggtggcaag ggagcagccg
    13141 tagcagtact gattttcgac gttctgaaag gaatgttgcc cgtctggggc gcgtatgaat
    13201 taggtgtcag ccccttctgg ttaggcttaa ttgccatcgc cgcctgtctt ggacacatct
    13261 ggcccgtttt cttcggattt aaaggaggaa aaggcgttgc taccgctttt ggtgccatcg
    13321 cacccattgg ctgggatctc accggagtaa tggcgggaac ctggttactg accgtgctat
    13381 tgagcggata ctcgtcgctg ggagcgattg tcagtgcact gattgctccg ttttatgtct
    13441 ggtggtttaa gccacaattc accttcccgg tttcgatgct ctcttgcctg atcctgctgc
    13501 gtcatcatga caacatccag cgtctgtggc gtcgtcagga gacaaaaatc tggacgaaat
    13561 tcaaaagaaa gcgcgaaaag gatcccgagt gatttctggt ggatctacat gacctgatag
    13621 ccttcatcgg gcttgcccag ccgttgctgg caccatgccg ccagaaattc cacgcagaca
    13681 cgtaatttca tgctgcgata gagcggctcc cggtaaacag cccagatatt ggcgctttgt
    13741 gcatactccg gcaatacttg caccagtttg ccactctcca gaaacggcaa cacatcccac
    13801 tcggaacgca gcataatccc tttgccctcc agcgcccatt gcaacactat ctcgccgcta
    13861 ttggaggaaa gatgcccgct aaccttcacc gattttttct cctgaccgtt ccccaactcc
    13921 catattccgt gggtcatatc gcgctctttg gtcaccagac agtcatgacg acttaattcc
    13981 tgtaatgatt gtggctgcgg atatttttgc agatactcag gtgctgcgca taatattctt
    14041 ttatttttcg ttaacagatg cgcaatataa taatcaggaa tttcgtcatt aatacgaata
    14101 tccagatcaa tattatcctg cactaaatca atttgccgat cgaacaattc aaaatgcacc
    14161 tgtaactcag gataattgcg cataagttcg gtaatggctg gcgcaatatg gctgcgccca
    14221 aaaccaaaac tgcaaccaat acgaatcatc ccctccgggc gcgttttgat ttacgtgacg
    14281 tcatcgacca gtcgctgata ttgggtgagg atttccagcg catgttcgta acaacgttgg
    14341 ccactttcag tcagcgccac gccgcgagcg gagcggttca gcagcgttgt tgccagcgta
    14401 ttttcaagga tttggatccg cttggtgaca aatgcaggcg tctgcccaag cgtcgccgct
    14461 gcggcgctaa aactcccgct gtggacgatt tctaccagta cctgcaagtc tttggctaaa
    14521 ggccagctat tcagcatcgt taattatccg cagttgtgat aagcgcagtg tatttcgcaa
    14581 aacattgcca ttccctgctc tggcgcaata ttcattctta ttttcgcgtt gattcacgaa
    14641 ttgttaatta catattcacg gtggcaaaaa atataaaacc acatttttag tggtagtttg
    14701 tggcggtgaa tttttccaga caaatacaaa aactggagtt gccatgatga gcgaaagtaa
    14761 taagcaacag gcagtgaata agttgacgga gattgtcgct aactttaccg ccatgatttc
    14821 tacccgaatg cctgatgacg tggtggataa actaaaacag ctaaaggatg ccgaaacgtc
    14881 gtcgatgggg aaaattatct accatacgat gttcgacaac atgcaaaaag cgattgacct
    14941 gaatcgtcct gcctgtcagg acaccgggga gattatgttc ttcgttaaag tcggttcccg
    15001 cttcccactg cttggcgagc tgcaaagcat actcaaacaa gccgtggaag aggcaaccgt
    15061 caaagcgcca ctacgtcaca atgcggtaga aatttttgac gaagtaaaca ccggcaaaaa
    15121 taccggtagc ggcgtaccgt gggtcacctg ggacatcatc cccgacaatg acgatgcgga
    15181 aatcgaagtt tacatggcag gcggcggctg cacgctacct ggccgctcga aagtgttaat
    15241 gccgtcagaa ggctacgaag gcgtggtgaa attcgtcttc gaaaatatct ccaccctcgc
    15301 cgtaaacgcc tgtccaccgg tactggtggg cgtgggcatc gccacctcgg tggaaaccgc
    15361 cgccgtactc tcgcgtaaag ccattttgcg cccgattggc agccgccatc ccaatccaaa
    15421 agcggcagaa ctggagctac gcctggaaga aggactcaac cgtctgggga ttggtccaca
    15481 agggctgact ggcaacagtt cagtgatggg cgtacatatc gaatctgccg cccgccatcc
    15541 gtcaaccatc ggcgttgctg tctctaccgg ctgctgggcg catcgtcgcg gcacactgct
    15601 ggttcatgcc gatctcacct ttgaaaatct gtctcacacc cggagcgcgt tatgaaaaag
    15661 atcctgacaa ccccgatcaa agctgaagat ctgcaagata ttcgcgtcga cgatgtgatc
    15721 tacctgaccg gtacgctggt gacctgccgt gacgtttgtc accgccgctt gatcgaactg
    15781 aaacgtccga tcccttacga tctcaacggc aaagcgattt tccacgctgg ccccatcgtg
    15841 cgcaaaaacg gcgataaatg ggagatggtt tccgttggcc cgacaaccag tatgcgtatg
    15901 gaaagttttg aacgtgaatt tatcgagcag accggcgtga aactggtggt tggcaaaggc
    15961 ggaatggggc cgctgaccga agaaggctgc cagaaattca aggcgctaca tgtgattttc
    16021 ccggcaggct gcgcggtgct ggcggcaacc caggtggaag agattgaaga agtgcactgg
    16081 acagagctcg gaatgccgga gtcgctgtgg gtctgtcggg tcaaagagtt cggcccgctg
    16141 attgtctcta ttgataccca cggcaacaac ctgatagccg aaaacaaaaa gctgttcgcc
    16201 gaacgccgcg atcccatcgt ggaagagatc tgcgagcacg tccattacat caaataaccc
    16261 tcccggagag gctcgccccc tctccttttt cgcaggcata acacgatgaa accttccact
    16321 gaatggtggc gataccttgc gccgctggcg gtcatcgcca ttattgctct aattccggtt
    16381 cccgcagggc tggagagtca tacctggctc tactttgccg tttttactgg cgtgatcgtt
    16441 ggactgatcc tcgaacccgt gccgggtgcc gtggtggcga tggtgggtat atccatcatc
    16501 gccatactct ctccctggct gctgttcagc ccggagcagc tcgctcagcc aggctttaaa
    16561 ttcactgcaa aatccctctc gtgggccgtt tccggttttt ctaattcggt tatctggctg
    16621 attttcgccg cctttatgtt tggcacaggc tatgaaaaaa ccgggcttgg acgccgcatt
    16681 gcgctgattc tggtgaaaaa gatgggacat cgcacgctgt ttctcggcta tgcggtgatg
    16741 ttctccgagc tgatcctcgc acctgtaaca ccgtccaact cggcgcgtgg tgcggggatt
    16801 atctatccca tcatccgtaa cctgccaccg ctctatcaat cacaaccaaa cgacagcagt
    16861 tcgcgcagca ttggctcgta catcatgtgg atggggattg ttgccgactg cgtgaccagc
    16921 gccattttct tgacggcgat ggcaccaaac ttgctgttaa ttggactgat gaaaagcgca
    16981 tctcacgcca cgctgagttg gggcgactgg ttcctcggga tgttgccgct cagtattttg
    17041 ctggtgctgc tggttccctg gctggcttat gtgctgtacc cgccggtact gaagtctggc
    17101 gatcaggtgc cgcgctgggc agagacggaa ctgcaggcaa tgggcccgct ctgttcgcgt
    17161 gaaaaacgga tgctggggct gatagtaggc gcgctggtgc tgtggatttt cggcggtgat
    17221 tatatcgatg ccgcgatggt cggttacagc gtggtggcac tgatgctgct tctgcgcatt
    17281 atcagttggg acgacattgt cagtaataaa gcggcgtgga acgttttctt ctggctggcc
    17341 tcgcttatca ccctcgctac cggactcaac aacaccggtt ttattagctg gtttggcaaa
    17401 ctgttagcag gcagcttaag cggttattcg ccaacgatgg tgatggtggc gttgattgtg
    17461 gtgttttatc tgctgcgcta ctttttcgcc agcgccacgg cgtatacctc cgccctcgca
    17521 ccgatgatga ttgccgctgc gctggcgatg ccggaaatcc cgctgccggt gttctgcctg
    17581 atggttggcg cggcaattgg tctggggagc attcttacac catacgccac cggacccagt
    17641 ccgatttact acggtagtgg ttatctgcca acggcggatt actggcgact gggggcgatt
    17701 tttgggctga tattcctcgt gttgctggtg attaccggct tactgtggat gcccgtggtg
    17761 ttgctttaag tgaggacggt gccggaaatt gctctccggc acccaatagt tttacgcagc
    17821 cggtaactcc gccagcggcc agcgcggacg cacgctaacg ccgagatccg ccgtcgcgcc
    17881 tgctttaaac cgcaccattc cggcataggc gatcatcgcg ccgttatcag tacaaaactc
    17941 cggacgcgcg tagaacactt cgccgcggcg ttttttcatc atttcagcca gcttcgcccg
    18001 taacgtgcgg ttagcactca cgccgcccgc catgaccagt cgcttaaagc ccgtctgatc
    18061 caacgctcgc ttacacttaa tcatcaacgt atcgaccacc gcatcttcaa aggcgcgggc
    18121 gatatcagca cgcgtctggt cgtcggtgcc gttgtcacga atcgtgttcg ccgcaaaggt
    18181 tttcagacca gaaaagctga aatccagccc cggacggtcg gtcatcggac gcgggaaaac
    18241 aaagcgcccg gcagtaccct gagccgccat tttcgacagt aacggtccgc caggataatc
    18301 cagccccagc agcttcgcgg ttttatcaaa cgcttcaccg gcggcatcat cgatagactc
    18361 gccgagcagc tcgtactgac caatgccagt cacgctgatt aactgcgtat gaccgccgga
    18421 caccagcagc gcgacaaacg gaaattccgg cgggttatct tccagcatcg gcgctaacag
    18481 atgcccttcc atatggtgta ccgggattgc cggaacgttc caggcaaacg ccagagaacg
    18541 ccccacggtt gcgccaacca gcagtgcgcc gactaatcca gggcctgcgg tataggccac
    18601 agcatcaatg tcttttgccg ttaagccaga ctcctttagc gccgcctgga tcaacggtac
    18661 ggttttgcgc acgtgatcgc gggaggccag ttcaggcacg acgccgccat agtcagcgtg
    18721 caatttcacc tgactataca attggttggc taacaaacct ttttcatcgt cgtaaatggc
    18781 gatgccggtt tcatcgcagg aagtttcaat acccagtaca cgcatgactt gttttacctc
    18841 gctttattac cgcgcagtgt aggaccaatg cgggttgatg taaaactttg ttcgcccctg
    18901 gagaaagcct cgtgtatact cctcaccctt ataaaagtcc ctttcaaaaa ggccgcggtg
    18961 ctttacaaag cagcagcaat tgcagtaaaa ttccgcacca ttttgaaata agctggcgtt
    19021 gatgccagcg gcaaaccgaa ttaatcaaag gtgagaggca catgccggta attaaagtac
    19081 gtgaaaacga gccgttcgac gtagctctgc gtcgcttcaa gcgttcctgc gaaaaagcag
    19141 gtgttctggc ggaagttcgt cgtcgtgagt tctatgaaaa accgactacc gaacgtaagc
    19201 gcgctaaagc ttctgcagtg aaacgtcacg cgaagaaact ggctcgcgaa aacgcacgcc
    19261 gcactcgtct gtactaattc cccgagagcg ttgctctccg atcagaccga gttgtagttg
    19321 taaggccgtg cttccgaaag gaatgcgcgg cttattttcg tttatgaatt gctaaaaatc
    19381 ggggcctatg gctggacgaa tcccacgcgt attcattaat gatctgctgg cacgcactga
    19441 catcgtcgat ctgatcgatg cccgtgtgaa gctgaaaaag cagggcaaga atttccacgc
    19501 gtgttgtcca ttccacaacg agaaaacccc gtcattcacc gttaacggtg agaaacagtt
    19561 ttaccactgc tttggatgtg gcgcgcacgg caacgcgatc gacttcctga tgaactacga
    19621 caagctcgag ttcgttgaaa cggtcgaaga gctggcagca atgcacaatc ttgaagtgcc
    19681 atttgaagca ggcagcggcc ccagccagat cgagcgccat cagaggcaaa cgctttatca
    19741 gttgatggac ggtctgaata cgttttacca acaatcttta caacaacctg ttgccacgtc
    19801 tgcgcgccag tatctggaaa aacgcggatt aagccacgag gttatcgctc gctttgcgat
    19861 tggttttgcg ccccccggct gggacaacgt cctgaagcgg tttggcggca atccagaaaa
    19921 tcgccagtca ttgattgatg cggggatgtt ggtcactaac gatcagggac gcagttacga
    19981 tcgtttccgc gagcgggtga tgttccccat tcgcgataaa cgcggtcggg tgattggttt
    20041 tggcgggcgc gtgctgggca acgatacccc caaatacctg aactcgccgg aaacagacat
    20101 tttccataaa ggccgccagc tttacggtct ttatgaagcg cagcaggata acgctgaacc
    20161 caatcgtctg cttgtggtcg aaggctatat ggacgtggtg gcgctggcgc aatacggcat
    20221 taattacgcc gttgcgtcgt taggtacgtc aaccaccgcc gatcacatac aactgttgtt
    20281 ccgcgcgacc aacaatgtca tttgctgtta tgacggcgac cgtgcaggcc gcgatgccgc
    20341 atggcgagcg ctggaaacgg cgctgcctta catgacagac ggccgtcagc tacgctttat
    20401 gtttttgccg gatggcgaag accctgacac gctggtacga aaagaaggta aagaagcgtt
    20461 tgaagcgcgg atggagcagg cgatgccgct ttccgcattt ctgtttaaca gcctgatgcc
    20521 gcaagttgat ctgagtaccc ctgacgggcg cgcacgtttg agtacgctgg cactgccatt
    20581 gatatcgcaa gtgccgggcg aaacgctgcg aatatatctt cgtcaggaat taggcaacaa
    20641 attaggcata cttgatgaca gccagcttga acgattaatg ccaaaagcgg cagagagcgg
    20701 cgtttctcgc cctgttccgc agctaaaacg cacgaccatg cgtatactta tagggttgct
    20761 ggtgcaaaat ccagaattag cgacgttggt cccgccgctt gagaatctgg atgaaaataa
    20821 gctccctgga cttggcttat tcagagaact ggtcaacact tgtctctccc agccaggtct
    20881 gaccaccggg caacttttag agcactatcg tggtacaaat aatgctgcca cccttgaaaa
    20941 actgtcgatg tgggacgata tagcagataa gaatattgct gagcaaacct tcaccgactc
    21001 actcaaccat atgtttgatt cgctgcttga actgcgccag gaagagttaa tcgctcgtga
    21061 gcgcacgcat ggtttaagca acgaagaacg cctggagctc tggacattaa accaggagct
    21121 ggcgaaaaag tgatttaacg gcttaagtgc cgaagagcga tcgggaagcc cccgacagcc
    21181 gcactgagag gcagcggcaa atatataagt acgccctcgt aattatcgtt ggcggtaaac
    21241 aaccgttgga tttcagcgtt aacggctgaa ggacatcggg tcaatcgccc aacaccaacc
    21301 tcatgaaata agtgtggata ccgtcttatg gagcaaaacc cgcagtcaca gctgaaactt
    21361 cttgtcaccc gtggtaagga gcaaggctat ctgacctatg ccgaggtcaa tgaccatctg
    21421 ccggaagata tcgtcgattc agatcagatc gaagacatca tccaaatgat caacgacatg
    21481 ggcattcagg tgatggaaga agcaccggat gccgatgatc tgatgctggc tgaaaacacc
    21541 gcggacgaag atgctgccga agccgccgcg caggtgcttt ccagcgtgga atctgaaatc
    21601 gggcgcacga ctgacccggt acgcatgtac atgcgtgaaa tgggcaccgt tgaactgttg
    21661 acccgcgaag gcgaaattga catcgctaag cgtattgaag acgggatcaa ccaggttcaa
    21721 tgctccgttg ctgaatatcc ggaagcgatc acctatctgc tggaacagta cgatcgtgtt
    21781 gaagcagaag aagcgcgtct gtccgatctg atcaccggct ttgttgaccc gaacgcagaa
    21841 gaagatctgg cacctaccgc cactcacgtc ggttctgagc tttcccagga agatctggac
    21901 gatgacgaag atgaagacga agaagatggc gatgacgaca gcgccgatga tgacaacagc
    21961 atcgacccgg aactggctcg cgaaaaattt gcggaactac gcgctcagta cgttgtaacg
    22021 cgtgacacca tcaaagcgaa aggtcgcagt cacgctgccg ctcaggaaga gatcctgaaa
    22081 ctgtctgaag tattcaaaca gttccgcctg gtgccgaagc agtttgacta cctggtcaac
    22141 agcatgcgcg tcatgatgga ccgcgttcgt acgcaagaac gtctgatcat gaagctctgc
    22201 gttgagcagt gcaaaatgcc gaagaaaaac ttcattaccc tgtttaccgg caacgaaacc
    22261 agcgatacct ggttcaacgc ggcaattgcg atgaacaagc cgtggtcgga aaaactgcac
    22321 gatgtctctg aagaagtgca tcgcgccctg cagaaactgc agcagattga agaagaaacc
    22381 ggcctgacca tcgagcaggt taaagatatc aaccgtcgta tgtccatcgg tgaagcgaaa
    22441 gcccgccgtg cgaagaaaga gatggttgaa gcgaacttac gtctggttat ttctatcgcc
    22501 aagaaataca ccaaccgtgg cttgcagttc cttgacctga ttcaggaagg caacatcggt
    22561 ctgatgaaag cggttgataa attcgaatac cgccgtggtt acaagttctc cacctacgca
    22621 acctggtgga tccgtcaggc gatcacccgt tctatcgcgg atcaggcgcg caccatccgt
    22681 attccggtgc atatgattga gactatcaac aaactcaacc gtatttctcg ccagatgctg
    22741 caagagatgg gccgtgaacc gacgccggaa gaactggctg aacgtatgct gatgccggaa
    22801 gacaagatcc gcaaagtgct gaagatcgcc aaagagccaa tctccatgga aacgccgatc
    22861 ggtgatgatg aagattcgca tctgggggat ttcatcgagg ataccaccct cgagctgccg
    22921 ctggattctg cgaccaccga aagcctgcgt gcggcaacgc acgacgtgct ggctggcctg
    22981 accgcgcgtg aagcgaaagt tctgcgtatg cgtttcggta tcgatatgaa caccgaccac
    23041 acgctggaag aagtgggtaa acagttcgac gttacccgcg aacgtatccg tcagatcgaa
    23101 gcgaaggcgc tgcgcaaact gcgtcacccg agccgttctg aagtgttgcg tagcttcctg
    23161 gacgattaat cggtaggccg gatcaggcgt tacgccgcac ccggcactag tccctctgca
    23221 caaacgccac ctttt
//
LOCUS       DADMKF010000055        23157 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.056, whole genome shotgun sequence.
ACCESSION   DADMKF010000055 DADMKF010000000
VERSION     DADMKF010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23157
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.056"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            231..1664
                     /locus_tag="JNC24_001989"
     CDS             231..1664
                     /locus_tag="JNC24_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HAY9158737.1"
                     /translation="MTQSRLHAAQNALAKLHEHRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALAPGDDNDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDCAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWTFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFLAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSVSPRTISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLVLWRYYPHENLDGYRSIPLPQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVLQHGALWLLG"
     gene            complement(1672..2730)
                     /locus_tag="JNC24_001990"
     CDS             complement(1672..2730)
                     /locus_tag="JNC24_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAY9158738.1"
                     /translation="MDLASDVCFCYSARFTANGLGMASLKDVARLAGVSMMTVSRVMH
                     NAESVRPATRDRVLQAIQTLNYVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDIL
                     LAIEQTASEFGWNSFLINIFSEDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYG
                     ENIVLANCVADDPALPSYIPDDYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQ
                     GFEQAWRDAGRDLAEVKQFHMATGDDHYTDLASLLNAHFKSGKPDFDVLICGNDRAAF
                     VAYQVLLAKGVRIPQDVAVMGFDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGR
                     EGGRVTRIPCPLLIRCST"
     gene            2898..3116
                     /locus_tag="JNC24_001991"
                     /pseudo
     CDS             2898..3116
                     /locus_tag="JNC24_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            3134..4462
                     /gene="dsdA"
                     /locus_tag="JNC24_001992"
     CDS             3134..4462
                     /gene="dsdA"
                     /locus_tag="JNC24_001992"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HAY9158739.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPGTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFAETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLDDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASGFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNTTHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(4570..6108)
                     /gene="emrY"
                     /locus_tag="JNC24_001993"
     CDS             complement(4570..6108)
                     /gene="emrY"
                     /locus_tag="JNC24_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HAY9158740.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPGLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQETMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRLLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(6108..7271)
                     /gene="emrK"
                     /locus_tag="JNC24_001994"
     CDS             complement(6108..7271)
                     /gene="emrK"
                     /locus_tag="JNC24_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HAY9158741.1"
                     /translation="MEQINSNKKQSNRKKYFSLLVIVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLALKRTDIRSPVTGY
                     IAQRSVQVGETVSPGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKDEDIAEMPELASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
     gene            7687..8301
                     /gene="evgA"
                     /locus_tag="JNC24_001995"
     CDS             7687..8301
                     /gene="evgA"
                     /locus_tag="JNC24_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HAY9158742.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGILVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            8306..11899
                     /gene="evgS"
                     /locus_tag="JNC24_001996"
     CDS             8306..11899
                     /gene="evgS"
                     /locus_tag="JNC24_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HAY9158743.1"
                     /translation="MKFLPYIFLLCCGLWSTISFADEDYIEYRGISSNNRVILDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQRVRGINADYLNLLKRTLNIKLTLRE
                     YADHQKAMDALEEGEVDIVLSHLVASPPLNDDIAATKPLIITFPALVTTLHDSMRPLT
                     SSKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGQNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVVLNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPDLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLNPGGWDILPGAIYSEDRENNVLFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIKVDNASAAFHKVKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDHAVYI
                     CGWQDITETRDLIHALEVERNKAINATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYSTGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALICSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLV
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEHPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFQALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPVSDDSKPEILQLLNSVKEHIAELDQEIAVFCQKND"
     gene            complement(11955..13100)
                     /gene="yfdE"
                     /locus_tag="JNC24_001997"
     CDS             complement(11955..13100)
                     /gene="yfdE"
                     /locus_tag="JNC24_001997"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HAY9158744.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLCNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     PGTMEKLGFSWERLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFDTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLAKIHEVGVPVAPLLSVAEAINLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(13174..14118)
                     /gene="yfdV"
                     /locus_tag="JNC24_001998"
     CDS             complement(13174..14118)
                     /gene="yfdV"
                     /locus_tag="JNC24_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HAY9158745.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(14188..15882)
                     /gene="oxc"
                     /locus_tag="JNC24_001999"
     CDS             complement(14188..15882)
                     /gene="oxc"
                     /locus_tag="JNC24_001999"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HAY9158746.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(15936..17186)
                     /gene="frc"
                     /locus_tag="JNC24_002000"
     CDS             complement(15936..17186)
                     /gene="frc"
                     /locus_tag="JNC24_002000"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HAY9158747.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDSAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(17699..18322)
                     /locus_tag="JNC24_002001"
     CDS             complement(17699..18322)
                     /locus_tag="JNC24_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HAY9158748.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQTQKTAAAER
                     ISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKTNLN
                     DDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVMENQY
                     LMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
     gene            18619..18894
                     /gene="ypdI"
                     /locus_tag="JNC24_002002"
     CDS             18619..18894
                     /gene="ypdI"
                     /locus_tag="JNC24_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis lipoprotein YpdI"
                     /protein_id="HAY9158749.1"
                     /translation="MKVNLILFSLFLLVSIMACNVFAFSISGGVSERSYKETEKTSAM
                     TTTHSTKLQPSQAILFKMREDAPPLNLTEEITPTYPTKANYLIHPVR"
     gene            complement(18971..19213)
                     /locus_tag="JNC24_002003"
     CDS             complement(18971..19213)
                     /locus_tag="JNC24_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HAY9158750.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            19566..20486
                     /gene="lpxP"
                     /locus_tag="JNC24_002004"
     CDS             19566..20486
                     /gene="lpxP"
                     /locus_tag="JNC24_002004"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HAY9158751.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNPKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            20842..20913
                     /gene="ypdK"
                     /locus_tag="JNC24_002005"
     CDS             20842..20913
                     /gene="ypdK"
                     /locus_tag="JNC24_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HAY9158752.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(20978..22216)
                     /gene="alaC"
                     /locus_tag="JNC24_002006"
     CDS             complement(20978..22216)
                     /gene="alaC"
                     /locus_tag="JNC24_002006"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HAY9158753.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDITEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKA
                     MFRADGLLPASSKHIHENAE"
     gene            22593..>23157
                     /locus_tag="JNC24_002007"
     CDS             22593..>23157
                     /locus_tag="JNC24_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HAY9158754.1"
                     /translation="MHEIFNMLLAVFDRAALMLICLFFLIRIRLFRELLHKSAHSPKE
                     LLAVTAIFSLFALFSTWSGVPVEGSLVNVRIIAVMSDGILFGPWVGIITGVIAGIHRY
                     LIDIGGVTAIPCFITSILAGCISGWINLKIPKAQRWRVSILGGMLCETLTMILVIVWA
                     PTTALGIDIVSKIGIPMILGSVCIGFIV"
BASE COUNT         6485 a         5420 c         5044 g         6208 t
ORIGIN      
        1 tactttggct gacatttttt tcccttaaat tcatctgagt tacgcatagt gataaacctc
       61 tttctcgcaa aatcgtcatg gatttactaa aacatgcata ttcgatcaca aaacgtcata
      121 gttaacgtta acatttgtga tattcatcgc atttatgaaa gtaagggact atatttttat
      181 aaaagttaac gttaacaatt caccaaattt gcttaaccag gatgattaaa atgacgcaat
      241 ctcgattgca tgcggcgcaa aacgcactag caaaacttca tgagcaccgg ggtaacactt
      301 tctatcccca ttttcacctc gcgccacctg ccggatggat gaacgatcca aacggcctga
      361 tctggtttaa cgatcgttat cacgcgtttt atcaacatca cccgatgagc gaacactggg
      421 gaccaatgca ctggggacat gccaccagcg acgatatgat ccactggcag catgagccta
      481 ttgcgctagc gccaggagac gataatgaca aagacgggtg tttttcaggt agtgctgtcg
      541 atgacaatgg tgtcctctca cttatctaca ccggacacgt ctggctcgat tgtgcaggta
      601 atgacgatgc aattcgcgaa gtacaatgtc tggctaccag tcgggatggt attcatttcg
      661 agaaacaggg tgtgatcctc actccaccag aaggaatcat gcacttccgc gatcctaaag
      721 tgtggcgtga agccgacaca tggtggatgg tagtcggggc gaaagatcca ggcaacacgg
      781 ggcagatcct gctttatcgc ggcagttcat tgcgtgaatg gaccttcgat cgcgtactgg
      841 cccacgctga tgcgggtgaa agctatatgt gggaatgtcc ggactttttc agccttggcg
      901 atcagcatta tctgatgttt tccccgcagg gaatgaatgc cgagggatac agttaccgaa
      961 atcgctttca aagtggcgta atacccggaa tgtggtcgcc aggacgactt tttgcacaat
     1021 ccgggcattt tactgaactt gataacgggc atgactttta tgcaccacaa agctttttag
     1081 cgaaggatgg tcggcgtatt gttatcggct ggatggatat gtgggaatcg ccaatgccct
     1141 caaaacgtga aggatgggca ggctgcatga cgctggcgcg cgagctatca gagagcaatg
     1201 gcaaacttct acaacgcccg gttcacgaag ctgagtcgtt acgccagcag catcaatctg
     1261 tctctccccg cacaatcagc aataaatatg ttttgcagga aaacgcgcaa gcagttgaga
     1321 ttcagttgca gtgggcgctg aagaacagtg atgccgaaca ttacggatta cagctcggca
     1381 ctggaatgcg gctgtatatt gataaccaat ctgagcgact tgttttgtgg cggtattacc
     1441 cacacgagaa tttagacggc taccgtagta ttcccctccc gcagcgtgac acgctcgccc
     1501 taaggatatt tatcgataca tcatccgtgg aagtatttat taacgacggg gaagcggtga
     1561 tgagtagtcg aatctatccg cagccagaag aacgggaact gtcgctttat gcctcccacg
     1621 gagtggctgt gctgcaacat ggagcactct ggctactggg ttaacataat atcaggtgga
     1681 acaacggatc aacagcggac aagggatccg cgtcactctt cccccttcac gaccttcaat
     1741 aatatgcaat gcagcttccc gcccgataat gtcatgtgga agctgaattg tggtcagcgg
     1801 cggtaaaaac agatgcccga cgccaaccag attatcaaag cccattacgg cgacatcctg
     1861 cgggatacgt acccccttcg ccaggagaac ctgataagcg acaaaggctg cacgatcgtt
     1921 accacatatc agaacatcaa aatctggttt gcccgatttg aagtgggcat tgagtaaact
     1981 tgcgagatcg gtgtagtgat catcacctgt tgccatgtga aattgtttca cctcagccag
     2041 atctcgtcca gcatcacgcc aggcctgctc aaatccctgc cgacgatacc ctgttgccaa
     2101 cgcactttcc ggtagccaga agcataacgg ttgacgatag cccgccgcga gcaaatgctg
     2161 tgttgattca tattgtgcag tgtaatcatc agggatataa ctgggtaacg ctgggtcatc
     2221 cgccacacag ttcgccaata caatattttc accatacaga gactcaggca gcgtgatatg
     2281 tcgcagcccc attgtagtat agataatgcc atccggacgg tgggcaagca gctgacgtgc
     2341 cgcgcgggca gcgtcatctt cagaaaaaat attgattaaa aaactattcc agccgaactc
     2401 gctggcggtt tgctcaatgg caagcagaat atcaacagag aaaggagtgg tagccgtgtc
     2461 ctgcgccagc acggcgagag tcgacggctt acgtccttga gcgcgcatct tacgggcgga
     2521 aagatcagga acataattca gggtctggat tgcctgcaat acgcggtcac gcgttgcagg
     2581 acgcacagat tctgcattat gcatcacccg ggagactgtc atcatcgaca ctcccgccag
     2641 gcgtgcgaca tcctttaatg aagccatacc caagccgttt gccgtaaaac gggcactgta
     2701 gcagaaacag acgtcactgg cgagatccaa cgccctatca cctgacacag caatacaata
     2761 aaaaataaca ataattcccg gacaattgtc cccaattccg cctctgttct cgccaacgag
     2821 taaccatata ggcaagtaag taaaacatgc caactggcat cgccaacctg gagaagaaaa
     2881 tataaatatg gatcattgtg ctgccgctgt atcccgacat cagcccggaa attattgcga
     2941 ttgctatcgg ttcaggtgca attggctgca cgatcgttac ggactcgctt ttctggctag
     3001 tgaagcaata ttgcggcgct acgctcaatg aaacatttaa atactatacg acagcgacat
     3061 ttatcgcttc agtcgtcgct ctggcgggca cattcctgct gtcatttatc atctaagcgc
     3121 aaagagacgt actatggaaa acgctaaaat gaactcgctc atcgcccagt atccgttggt
     3181 aaaggatctg gttgctctta aagaaaccac ctggtttaat cctggcacga cctcattggc
     3241 tgaaggttta ccttatgttg gcctgaccga acaggatgtt caggacgccc atgcgcgctt
     3301 atcccgtttt gcaccctatc tggcaaaagc atttgctgaa actgctgcca ctggggggat
     3361 tattgaatca gaactggttg ccattccagc tatgcaaaaa cggctggaaa aagaatatca
     3421 gcaaccgatc agcgggcaac tgttactgaa aaaagatagc catttgccca tttccggctc
     3481 cataaaagca cgcggcggga tttatgaagt cctggcacac gcagaaaaac tggctctgga
     3541 agcggggttg ctgacgcttg atgatgacta cagcaaactg ctttctccgg agtttaaaca
     3601 gttctttagc caatacagca ttgctgtggg ctcaaccgga aatctggggt tatcaatcgg
     3661 cattatgagc gcccgcattg gctttaaggt gacagttcat atgtctgctg atgcccgggc
     3721 atggaaaaaa gcgaaactgc gcagccatgg cgttacggtc gtggaatatg agcaagatta
     3781 tggtgttgcc gtcgaggaag gacgtaaagc agcgcagtct gacccgaact gtttctttat
     3841 tgatgacgaa aattcccgca cgttgttcct tgggtattcc gtcgctggcc agcgtcttaa
     3901 agcgcaattt gcccagcaag gccgtatcgt cgatgctgat aaccctctgt ttgtctatct
     3961 gccgtgtggt gttggcggtg gtcctggtgg cgtcgcattc gggcttaaac tggcgtttgg
     4021 cgatcatgtt cactgctttt ttgccgaacc aacgcactcc ccttgtatgt tgttaggcgt
     4081 ccatacagga ttacacgatc agatttctgt tcaggatatt ggtatcgaca accttaccgc
     4141 agcggatggc cttgcagttg gtcgcgcatc aggctttgtc gggcgggcaa tggagcgtct
     4201 gctggatggc ttctataccc ttagcgatca aaccatgtat gacatgcttg gctggctggc
     4261 gcaggaagaa ggtattcgtc ttgaaccttc ggcactggcg ggtatggccg gacctcagcg
     4321 cgtatgtgca tcagtaagtt accaacagat gcacggtttc agcgcagaac aactgcgtaa
     4381 taccactcat ctggtgtggg cgacgggagg tggaatggtg ccggaagaag agatgaatca
     4441 atatctggca aaaggccgtt aataacgttt caacgcagca tcgcaatcct ttccctgggt
     4501 gagcgatgct gccgatggcg cagacttaag atccccggtc ttacccgcta taaccccctc
     4561 cttttatcat cacccaacgc ctttcgctgt aaacggcggt ttcgcaaacc aaaccaaaac
     4621 cgtaagtaag ataaaagcaa tagccgccat acggaaaatc tcatttgcag aaatagaaag
     4681 tgactgctgg gttatttcat tattaatttc attaagaact cctgaaagcg aaccataata
     4741 tttatccata atttgtgacg atgaattaaa cacggggtta aattgatcga tggttgctgt
     4801 caactgacta tggtgtaacg attcgcgtcg tccccacagc gtcattgtca acgacgtacc
     4861 aactgatcct gacaaggtac gaaaaaaatt actcatactc gaggcattgg caaatttatt
     4921 atctggcaag cctgaaaacg aaatcgttgt taagggtaaa aagaaacagg caacggcgaa
     4981 tccctgaaaa aactgcggca aaatgatgcc tgtaaaatca atcgttggca taaatgtcac
     5041 agaacgccag taatagcaaa ccgcatacat caaaaaacta aatgtcacta acaaccgcat
     5101 gtctattttg ttgccataac gtcctatcaa aggtgaaatt aatagtggca tgatgccgat
     5161 gggcgcataa gcaagtccgg cccatatcgc attatacccc atcgtttcct ggagtaactg
     5221 cggcataagg acgatcgctc cagagtaaaa taaatacgcg catgtgatac tcacaatacc
     5281 aatggtgaag ttacgggact taaacaaact gagatcaaga atcgggttct ctgaggtcga
     5341 ctcccaaatg actaaagaga tcagagaaat aactgatact actgttaata ttattattgt
     5401 actcgagttg aaccaatcca gatcgcgccc tttatcaagc ataatttgca agccaccaac
     5461 accgagcact aacagggtca gtcctggtag attcattttg accggtgaag tctcagtttc
     5521 tcttccttta agtaaggtta agcataatgt caggacgata atccccatag ggacattgat
     5581 taaaaatatc caaccccagc taaagttatc acaaatataa ccgcccaata tcggcccaca
     5641 tatcggagcg ataatcacgg tcattgacca taatgccaga gcaaatgttc ttttttctgg
     5701 cggataattc cttaataata aactctgtga cagtggaatt aacggccccg ccattaaccc
     5761 ctgaacgact ctaaaaaata tcagcacatc aagattggtc gataggctac acattaatga
     5821 agacagcgaa aaaaaggtta ctgaaagtaa aaataatctt aattcgccta ttctttgtgc
     5881 caacctgcca gtaacaggga tcgcaatggc atttgctaca ccaaacgagg tgataaccca
     5941 ggtgccttcg tctgttgatg ctcccagaaa gccagatatt gtcggtattg cgacgttaga
     6001 aatagtggaa tccaacattt gcataaatgt cgctaatgac aatgcaatag tgacgcacca
     6061 taacgtccca ccggttaatg gtgccggagt tgatttagtg attgccatta aagttgtcca
     6121 ttatgcgaaa taatgttgct aatttctttt tctatcggac tggtatcgat aactaaagcc
     6181 ttactggtat aagccggcat ggaggtcacg gttgaagcca gctcaggcat ctcagcaatg
     6241 tcttcgtcct tcgtatcaat agttgcagtc atcgataaac caatgcgcaa ggggtgttcc
     6301 atgagttctt ttggatcaag agaaacttca accggtacac gctgaacgat tttgatccag
     6361 ttccctgtcg cattttgtgc aggtaataag gagaacgcat tgccggttcc catattgatc
     6421 cctgtcaccc gaccatgaaa cacaacattt tcaccataaa gatcgctgat aatattgact
     6481 gattgaccaa tccgtacatc cgtgagttgt gtttctttaa agttggcatt aacccacatt
     6541 tgacgtgccg gtacgacagc cattaacgat tgtccgggac tcactgtttc gccgacctga
     6601 acacttcgct gggcaatata gccagtaacc ggactcctta tatccgtacg tttaagcgcc
     6661 aaccaggctt ctttggttgc atccgccgct tcaactactt gtggctgacg gtttaatggt
     6721 gtgttcatta ctaaagcttt attcgcttta taagcctgga tagcggcatt cagtgccgct
     6781 ttgctactta ttaacgtatc tttggtatgc tccagcgttt cttttgaaat aaccccctgc
     6841 ttcgctaaag gcacacgacg gttataatct tctaaggatt gttgatactg aatacgtgct
     6901 gaagcgactt cggcactgta ttgtttatcc tgtaagtaca gtttattcgt ttgccgaaca
     6961 atatttgcca gattattttt agctttattg agtgcgatag tggcatcagt tttatccagt
     7021 gaaactaaaa tgtcaccctg tcgaacgtag ttcgtatctt tatgattaac tacagtgaca
     7081 ctacctgaga cttgtgcaga aattggatct gcattccccg tgacataggc gtcatctgta
     7141 ctaatcatgt cttctaattc cattgaccaa taggcatagg cacctgaaaa cgcaataaat
     7201 aaaactatca ccaataaaga aaagtatttt tttctgttag actgtttttt atttgaatta
     7261 atctgttcca ctattatctc tcatttcgca tagatgaaat ttatgaattg aatgaaaaga
     7321 gtattcattc ttcatatcaa acacagtgca tgtgttgcac attatcccag gaacgttgga
     7381 ggcctaattt aaatgaggtg caaaaaaaac aacagaggac taaaccgtgg cttttgcaat
     7441 acaattctta cgcctgtagg attagtgaga agacttatag tgctaacttg aaactataaa
     7501 tcatcggtat aatccctgat tttattgttg acatttcatt tatgccgact atttatatgg
     7561 tatacttgtc gaattatctt aaaggaagct cagattttct tatttttatt gagaaaatga
     7621 gatgacgcct tatgtctgta ttactacagg gagaagggag atgcttcatt gcaaagggaa
     7681 taatctatga acgcaataat tattgatgac catcctcttg ctatcgcagc aattcgtaat
     7741 ttattgatca aaaacgatat tgaaatctta gcggagttaa ctgaaggcgg aagcgccgtt
     7801 cagcgggtgg aaacacttaa gcctgatatc gtcatcattg atgtcgatat ccccggagtt
     7861 aacggtatcc tggtgttaga aacgctgagg aagcgccaat atagcggaat tattattatc
     7921 gtctccgcta aaaatgacca tttttacggg aaacattgtg ctgatgctgg cgctaatggt
     7981 ttcgtgagta aaaaagaagg catgaacaat atcattgcgg ctattgaagc tgcaaaaaat
     8041 ggctactgct atttcccctt ctctctcaac cggtttgttg gaagtttaac gtccgaccag
     8101 caaaaactcg actccttatc gaaacaagaa attagtgtca tgcggtatat tcttgatggc
     8161 aaggataata atgacattgc ggaaaaaatg ttcatcagca acaaaactgt cagcacttat
     8221 aaaagtcgcc tgatggaaaa attagaatgt aaatcactga tggatcttta cacattcgca
     8281 caacgtaaca aaatcggcta accacatgaa gtttttaccc tatatttttc ttctctgttg
     8341 tggtctttgg tcgaccataa gtttcgcaga cgaagattac atcgaatatc gtggcatcag
     8401 tagtaacaac cgtgtcatac ttgatccagt acgtctgagc aacaaggaat tacgttggtt
     8461 agcgagcaaa aaaaatcttg tgattgcagt acataagtcc caaacggcta cgttgttgca
     8521 taccgattcg cagcaacgag ttcgtggtat taatgctgat tatttaaatc ttttaaaaag
     8581 aacgttaaat atcaaattaa cactccggga atacgccgat catcaaaaag caatggacgc
     8641 gctggaagaa ggtgaagtcg atatagtgtt atcacattta gtagcttcgc cgcctcttaa
     8701 tgatgacatt gctgcaacca aaccactgat aattaccttt ccggcgctgg taaccaccct
     8761 tcacgattca atgcgaccgc ttacctcatc aaaaccagta aatattgctc gagtagcaaa
     8821 ttatccccct gacgaggtaa ttcatcaatc atttccaaaa gcaacaatta tctcttttac
     8881 aaatttatat caggcattag catccgtctc agccggacag aatgattact ttattggtag
     8941 taacatcatt accagcagta tgatttcccg ctatttcact cactccttaa atgtagtgaa
     9001 atattataac tcaccgcgtc aatataattt tttcttgacc agaaaagaat ctgtcgttct
     9061 taatgaagta ctcaatcgat ttgttgatgc tttgacaaat gaagttcgct atgaagtatc
     9121 acaaaattgg cttgatacag gaaacctggc ctttctgaac aaaccattag aactcactga
     9181 acatgaaaaa caatggatta agcagcatcc cgatttaaag gtgctggaaa atccttactc
     9241 ccctccctat tctatgacgg atgaaaatgg ctcggttcgg ggcgttatgg gggatattct
     9301 taatattatt actttgcaaa caggtttaaa tttttctccg atcaccgttt cacacaatat
     9361 ccatgctgga acacagctga atcccggcgg atgggatata ttacccggtg ctatttatag
     9421 tgaagataga gaaaataatg ttttatttgc tgaagccttc ataacaacgc cttacgtttt
     9481 tgtcatgcaa aaagcgcctg acagtgaaca aacattaaaa aaaggaatga aagttgccat
     9541 tccatattat tatgagcttc attcgcaatt aaaagagatg tatccggagg tagagtggat
     9601 aaaagtcgat aacgccagcg ctgcatttca caaggtcaag gaaggcgaac ttgatgctct
     9661 ggtcgcgaca cagttaaatt cacgttacat gatcgaccat tactatccta atgaacttta
     9721 tcattttctt attcccggcg ttccgaatgc atcgctttcg ttcgcttttc ctcgcggaga
     9781 accggaactt aaggatatta ttaataaagc actgaatgca atccccccaa gcgaagttct
     9841 gcgcctgacc gaaaaatgga ttaaaatgcc caatgtgacc attgacacat gggacctcta
     9901 tagcgagcaa ttttatattg ttacgacatt atccgtttta ttagttggca gtagcctttt
     9961 atggggattt tacctgttac gctcagttcg tcgtcgtaaa gtcattcagg gtgatttaga
    10021 aaaccaaata tcattccgaa aagcactctc ggattcctta ccgaatccaa cttatgttgt
    10081 aaactggcaa ggtaatgtca ttagtcataa tagtgctttt gaacattatt tcactgcgga
    10141 ttactacaaa aatgcaatgt taccattaga aaacagtgac tcccccttta aagatgtttt
    10201 ttctaatgcg catgaagtca cagcagaaac gaaagaaaat cgaacaatat acacacaggt
    10261 ttttgaaatt gataatggca tcgagaaaag atgcattaat cactggcata cattatgcaa
    10321 tctgccagcc agcgatcatg ctgtttatat ttgtggttgg caagatatta cagagacgcg
    10381 tgatttaatt catgcactcg aagtagagag aaataaagcg atcaatgcaa ctgtcgcaaa
    10441 aagtcagttt ctggcaacaa tgagtcacga aataagaaca ccaataagct ccattatggg
    10501 cttcctggaa cttctgtcgg gttctggtct tagcaaggag caacgggtgg aggcgatttc
    10561 acttgcctac tccaccggac aatcactcct cggcttaatt ggtgaaatcc ttgatgtcga
    10621 caaaattgaa tcgggtaact atcaacttca accacaatgg gtcgatatcc ctactttagt
    10681 ccagaacact tgtcactctt tcggtgcgat tgctgcaagc aaatcgatcg cattaatttg
    10741 cagcagtaca tttcctgatc attacctggt taagatcgac cctcaggcgt ttaagcaggt
    10801 cttatcaaat ttactgagca atgctctcaa atttaccacc gagggggcag taaaaattac
    10861 gacctccctg gttcacattg atgacaacca cgctgttatc aaaatgacga ttatggattc
    10921 tggaagtgga ttatcgcagg aagaacaaca acaactcttt aaacgctaca gccaaacaag
    10981 tgcaggtcgc cagcaaacag gatctggctt aggcttaatg atctgcaaag aattaattaa
    11041 aaatatgcag ggcgatttgt cattagaaag tcatccaggc ataggaacaa catttacgat
    11101 cacaatcccg gtagaaatta gccagcaagt ggcgactgtc gaggcaaaag cagaacaccc
    11161 catcacacta cctgaaaagt tgagcatatt aatcgcggat gatcatccga ccaacaggct
    11221 attactcaaa cgccagctaa atctattagg atatgatgtt gatgaagcca ctgatggtgt
    11281 gcaagcgcta cacaaagtca gtatgcaaca ttatgatctg cttattactg acgttaatat
    11341 gccgaatatg gatggttttg agttgactcg caaactccgt gagcaaaatt cttccttacc
    11401 catctggggg cttacagcca acgcacaggc taacgaacgt gaaaaagggt taagttgcgg
    11461 catgaactta tgtttgttca aaccgttgac cctggatgta ctgaaaacac atttaagtca
    11521 gttacaccag gttgcgcata ttgcacctca gtatcgccac cttgatatcg aagccctgaa
    11581 aaataatacg gcgaacgatc tacaactgat gcaggagatt ctcatgactt tccagcatga
    11641 aacgcataaa gatctacccg ctgcgtttca agcactagaa gctggcgata acagaacttt
    11701 ccatcagtgt attcatcgca tccacggtgc ggctaacatc ctgaatttgc aaaagttgat
    11761 taatattagc catcagttag aaataacacc tgtttcagat gacagtaagc ctgaaattct
    11821 tcagttgctg aactctgtaa aagaacacat tgcagagctg gaccaggaga ttgctgtttt
    11881 ctgtcagaaa aatgactaaa tagcggctcc cacaatgttc taatgtggga gctatttacc
    11941 agcacatctt acatttatga tgagaactcc tggcgaattt gttccccatg ctggtcgagc
    12001 gttgccgctc ccggcataac atgcgggtcc gcgcagccgc tgatttttat cggattaccc
    12061 ggcatcatta ttcctccggc ttcaatcaac atatttctcg cctgagtttg tggcaaatta
    12121 atggcctcag ccacacttaa taacggcgcg acgggcacac caacttcatg tattttggct
    12181 aaccaaactt cagctgcctg cgtttttaac gttcgctcaa tatattgttt aagaatagcc
    12241 tggttttgta cgcgtaaaat attgctgcta aatcggggat cattaaccag ttccgtaagc
    12301 tccagtgcct ggcataacgc agaaaaaagc ttgtcattac cacaacaaat cgtaatcggc
    12361 ttatcctgag tatcgaaaac atcaaaaggt gccatgtagg gatggcgatt tcccaggcgt
    12421 tgtggtgact tccctgtcgc aatatatgcc atcagaccat gctccagaaa actcagcgtg
    12481 gcatcaaaca tcgctatatc gacatgcgcc cctctctggc tcttttcgcg gccataaagt
    12541 gcactcacta ttccgctgaa taaatagaca ccgccgcata gatccgcaag agaggtacca
    12601 acacgcactg gcggagcatc agggtaccct gtttccatca ttatcccact cattgcctga
    12661 atgatggtat cgtaggcagg agcatctttt agcggaccgg tatgtccgaa acctgacgat
    12721 gaagcatata tgaggcgcgg gttgatttct tgtaaccttt cccatgaaaa ccccagtttt
    12781 tccattgtac ctgggcgaaa attctcagct aatacatcag cttgtttgag catatttata
    12841 aatatacttt tatcgtgatc attctttaaa tcaagaacca cactctcttt gccatgatta
    12901 ataaaactgt aatagagtga ctgtccatcc acatagggac caaatgtgcg ggtatcatca
    12961 ccatgacctg gtggctcaac tttaattacc cttgcgccca tattacaaag aagttgagtt
    13021 ccgaaaggtc cattaaggac atgtgtcata tcgataacta ataagccttc aaacggccct
    13081 ttgctttcat tatttgtcat ttgcttatgc cttgaaaaaa gccgaaggtg aaaagacact
    13141 tcggcattta tgtaaaatca ataacatgat taattatgaa accagtcgac tgacataaat
    13201 ccacaaggga gccgtgacga caaagcccag tacgctcaca gccaatgacg ctgtaccagt
    13261 acgggtgtag acattaaacc ggctggcaat aatgattccg gagaatgccg gcggtaatgc
    13321 gcctgccagt accatcatct gcaaatgttc gctgttcaaa tgacatgcca taccaacgag
    13381 aagcagcgcc agtggcatca gaatcaactt caggaaggtg ttataagcaa tttcggcact
    13441 gaactcgaat ttatgtgctg ccagagtcaa ccccgcagcg aataccgcta cccctgagtt
    13501 agccttcgca atcagattaa aggtcgggtc ccatgctgcc ggaattttta ccccaaccag
    13561 caccaggatc gttgccagaa caggtgccca tactactggc tcctttgccg cagaaattaa
    13621 tgcgctcaga ttactattct tcttaccatc cgctcctgag gaaggattca gcaaatacag
    13681 accaatagga atagtaattg cgttaacaat aatagaaata attgctacca ctaaacctgt
    13741 tgatacggaa tcaccataaa taggatcgag aactgcaaac cccaggaatc caatggtagg
    13801 tgaacccgca attaatgcac atacagcggc ttctgcatgg gtacgtttaa aaaatttgta
    13861 gcaaccaaac caagagaaaa agaaacatcc gacaataacc acaagtgata caagggtcag
    13921 acgagtgtcc gcaaaaatca tttccctgtt tgcccgggta atagaaacaa atagagccgc
    13981 aggaagagcg tagtttaata ccagtttatt aaatgcgcgg gcttgatctt ctgaaaaagt
    14041 ttctcgtctg ccgctaaaat aacccaacag catgattacg ataataggca ataaatcgcc
    14101 aataaaaaat gttagcataa tagtatttcc gataagggtc ccctcaccat cgcataatga
    14161 gttggcaaga ggagacgatg tcagtattta attaccagcg acttgttttg ggttaagttt
    14221 ggtaatatgg ccactttcag ttcctgcagc agggtcgatg accacattaa taatggtcgg
    14281 tttgcgcgac tgaataccgg tggttaaagc atgacgaagt tcatctgtcg tggtgacgtt
    14341 atagccaacg ccacgaaacg catccattaa tttgtcatac cttgcatggt gcaacagatc
    14401 cgttggtgat ggtgcaccag cgccactgag atcaacaccg tctcctctgt agatgccgcc
    14461 attattaaaa ataacgatcg tcaccggcag gttatatcga caaatcgttt caatttccat
    14521 cccactgaaa ccaaaagcac tatcaccttc aatggcgaca accggagaac cagaagtcac
    14581 gctagcaccg atggcatagc ccataccgat gcccatgaca ccccaggtac cacaatccag
    14641 acgacgacgt ggtttataca tatcaataat atttcgtgca ttatccaggg tatttgcacc
    14701 ttcattaact aaataaatat cctggttctc gcgcaatacg tcgcgcacag cacttaatgc
    14761 attaaagtaa tttaatggtt gagtatctgt acttaatttt tcatgcattt tttgtgcatt
    14821 ttgctgcttg tggatattta aaatatcgcg ccataccagt ggagtcgtaa atgtgttttg
    14881 tttcagttct gccagcatac cttgcatact ggatgcaata tcaccaacga ctggcacagc
    14941 aatggggcgg ttgctgtcaa tttcctgcgg ttcaatatcc agttgaataa actgtgtatc
    15001 tgccgcccat ccttttttac cgtgtgccaa taaccaattc agtcgtgcac caacaagcat
    15061 gacaacgtca gcatttgcca gggcaaacga acgcgcagct gccgcagaaa gtggatgcgt
    15121 atcttcaagg atccctttcg ccatagacat tggcaggaat ggaatctgag cactttcaat
    15181 aaattcacga agctgttcat cagcttgtga atacgccgcg cctttgccaa ggataattaa
    15241 tggccgttca gcttttgcta aaagcgaaat tgcgctagtg actgacttcg ggcatggcaa
    15301 taatgctggc gacggatttt caactttaac aatcgtggtt aacgcttcgt ctttttccat
    15361 cgtcgcggcc aggacatttg ctggcaaatc aagataaact ccgccagggc gacccgatac
    15421 agagacccgg atagcgcgtg ccaatgcaat gccaagatcc tgcggctgat taacgcgaaa
    15481 tgctgctttg gcatacggtt ttgccgcatt catttggtcc agctcttcat aatcaccttg
    15541 ctgtaggtcg acgatcgcgc ggtcgctgga gccgctaatc atgatcatcg gaaaaccatt
    15601 taccgttgcg ttggccaatg cggtcaaacc attgaggaat cctggcgcag aaactgtcag
    15661 gcagatcccc ggtttttggg taagaaaacc gcttgccgca gcggcatagc ctgccgactg
    15721 ctcatgacga aaaccaatat aacgaatgcc ttccgcctgg gcatggcgtg ccatatccgt
    15781 cacaggaata cctacaacac cataaatagt gtcaatatta ttctgtttta atgcttcaac
    15841 gatgatatgc ataccatctg tcatttgaag ttgatctgac atgattaata acctcgaaat
    15901 ttttataaat tgggagccag aagcccccgt taatatcaga tggcgtggtt ttgcttcatt
    15961 gcagcaattt catcgtcgct ataacccagc tcctgcaata cagcagcggt atgttcacct
    16021 aatagcggcg cagctttaat atccggcgta aaggcagaga atttcattgg acaaccaacg
    16081 gtcagatatt ttccacgcaa cggttgttcc acttcaacaa cactgccact ttggcgcaaa
    16141 gagggatcaa gtgaaatttc tttcatactt aaaaccggtg cacaaggaat atcaaactga
    16201 gtcaaatagg ccaccgcttc atgtttatca atagtgacag tgtatttttc gatttcagca
    16261 aaaatatcga aaatatgtgg ctgtcgggca tgggctgtac tgtatgccga atcggtaatc
    16321 cattctggtt tgccgatggc tttacaggtg ttttcccagt tttgctcctg aatagtgaaa
    16381 taaatatagg cgttaggatc ggtttcccag cctttacatt tcaggatcca gccaggctga
    16441 ccgccaccac ctgcattacc accgcgggga actgcatcac caaatgtacc attcggatac
    16501 tgcgggtatt cttccagata acccaattta tcgagacgct gctggtcacg taatttcacg
    16561 cggcaaaggt tcaatacggc atcctgcatt gacatggtga ctcgttgccc acgccccgtt
    16621 ttttcgcgat gcagcaaagc agcaagtaaa ccgatcagca aatgcattcc ggtgttgctg
    16681 tcacccaacg ctgcagcgct taccagcggc ggaccatccc aaaaacccgt agtggatgcc
    16741 gcgccacccg ctgcctgagc aacgttttca taggctttta cattcacata aggcgaacac
    16801 tcatcaaacc ctttgatcga accaaaaatc agacgtggat tgatttcttg aatatgctcc
    16861 caggtgaagc ccatgtgatc aatggcccct ggatgaaagt tctcgactaa gatatcagct
    16921 tcgcggatca gcttttccat tacctctttg ccttccgctg ttttggtatt taactcaata
    16981 gaacgtttgt tactgttaag catggtgaag taaagcgcat cgatatcagg aatatctcgc
    17041 agttggtgac gcgttacgtc accaacgccg ggacgttcaa ttttaataac gtcagcgcca
    17101 aaccaggcca gcatttgagt acaagatggg ccagattgca caccggtgaa atcgagaact
    17161 ttaattcctt gaagtggagt tgacatagca atacctatta gttaattaaa tatgtgttaa
    17221 tttagcgttt atttaaggca taacgccctc tataaataat ctgcaaaata tcttcgtagc
    17281 ggcactcgat atttttaaat tcctgtatca agtactgaaa attatttcat cgccacttcc
    17341 aattcctttc tgcatggtaa gtagtgagga cgaaaagaag aatatgataa atatttattc
    17401 gtggtaatca gagagagtcg cgacatttgt gatgttattc ttacaactgt aagaagcgcc
    17461 ggtgatttac aagtaagagt atgattacgc tttttaaagc gtaccataaa gcataacata
    17521 ataaatgatt tgtgatgaca ctggattatt gcggtttact ttgaggaaaa taaaatgcgt
    17581 gtttatagaa ccgcgagcgc caaataattt tatgaccaca acctgtcgta ttgtggtcat
    17641 aaaaaattgt cggctggagg gagttaaatc ctttacctca atatactgcc tgtcagcatt
    17701 agttcacaaa cagtgcttcg ctatcgacga tgattccatc ttccgcacct ttaagcgcca
    17761 ggtttgcctc gtagtattgc tttttatcaa gcagtttctg cgcatcagca agtgcattac
    17821 gagtctgttt aagcggcatc agatattggt tttccattac gccaacaccg gccaggcgaa
    17881 gctcttccat tgcgcccttt ttatcccctt tcgccatttt ttcattggca atttttatcg
    17941 cggcttcttt ttcgggtgtt gctacataac tctcagaaat tccgacagat gcattaatga
    18001 caatatattg gtcatcattg agattggtct ttttacctgg cttagcgaac tttgcccatt
    18061 cggtactgtc atctgacagc aaagcggaag cttcattggt cagttctttt gccttttccg
    18121 gatcgccatg aaacagagcc aggcgcgcta cctgcacgtc acgcatcgca tataaacctt
    18181 gttcagagat acgctctgct gctgccgttt tctgggtttg ctgagtttgc tgttgtgccg
    18241 ctaccggtgc attatcagca gcccatacag ttgaagatgc cagaattgct gtgaccatcg
    18301 tagccataat taaacgtttc atacttacct cattctttaa cagttttaga aaacgccgcc
    18361 atcatattgg gtgagcatga aatgagaaat cagagagcaa cggtcatttt gaaagggtta
    18421 tcttacaatt gtagttatta tcgcaaagcg gcatttagtc ttacagttgt aggaatatgc
    18481 ttcctgtcgc agagaactat tcttatatac cacaaagaga tgtcatttat tatattgatg
    18541 taaatattga ttaattatat gtaaatacag ccataacatc gtatggttct cagtgaacca
    18601 ttttcaggat aactttctat gaaagtaaac ttaatacttt tcagcttatt tttattggtc
    18661 tctattatgg catgcaatgt ttttgcattt tccatttctg gtggtgtaag tgagaggagc
    18721 tataaagaga ctgaaaaaac atcagcgatg acaaccacac actctacaaa acttcagcca
    18781 tcacaggcga ttttgtttaa gatgagagaa gatgcgccac cattaaacct cacagaagaa
    18841 atcacgccta cttatccgac aaaggcgaat tatcttattc atcctgtgcg atagcgtctg
    18901 tcacatggca ggtgaacaat catgtctgat gctgatgtga acacctgccc aaatgagcca
    18961 tcctgaccca ctatcgcctg ccatacccca ccataaagcg aatcatcaag ccacaagccg
    19021 gggcaagaaa cataaatatc cccgtcacca gttcattacc gccagttaac cacatggtga
    19081 agtaataaat gagtgcagcg aggatcacca tgccgaagaa agaagagacc gccgatacca
    19141 cgttcagtac ctgaccgatg tagccgctta cgcagacaat acacagggcg agaaacatcc
    19201 ataaataaat catagctcaa cctcctgccg cgctaaaaaa cctgtaactt cagtcacttt
    19261 aatagtaaga caatccttaa cctctggcaa tttttgctaa gcatcaatga aaacagcacg
    19321 ttaaatgcaa cgctattgcg tgcggaaaat tttttacttt gcacgattaa taatcagtag
    19381 ctgaaagcag tcagccagtg ataaagtaga gccaccgcat tcatgctcct cgtggttatg
    19441 tcctgactag tctttacact ctttacagga accattgtcg tacatgatgg cccaaccaat
    19501 tgagtgtacc gctgtcgaca tggaatcttc tgcgatacaa caattcgtat ctacagaagg
    19561 taactatgtt tccacaatgc aaattttccc gcgagtttct acatcctcgc tactggctca
    19621 catggtttgg gcttggtgta ctctggcttt gggtacagct tccttatcct gttctctgct
    19681 ttctcggcac gcgtattggc gcaatggcgc gaccattcct gaaacgtcgt gaatctatcg
    19741 cccgtaaaaa cctggaactt tgtttcccgc agcattctgc ggaagaacgc gagaagatga
    19801 ttgccgaaaa ctttcgttca ctcggcatgg cgctggtaga aaccggcatg gcatggttct
    19861 ggcccgacag tcgcgtacgt aaatggtttg atgttgaagg gttggataac cctaaacgcg
    19921 cacaaatgca aaatcgcggc gtaatggttg tcggcgtcca ttttatgtcg ctggaactgg
    19981 gcggccgcgt gatgggactg tgccaaccaa tgatggctac ctatcgtcca cataataatc
    20041 agctgatgga atgggtgcag acccgtgggc gcatgcgctc taacaaagcg atgatcggca
    20101 gaaataatct gcgcggcatt gtcggtgcac tgaagaaagg tgaagcggta tggtttgctc
    20161 ccgatcagga ttatggtcgt aaaggcagct ccttcgcgcc gttctttgcg gtggaaaatg
    20221 tcgccacaac caatggcacc tatgttctct cccgtctctc tggcgcagcc atgttgaccg
    20281 taacgatggt aagaaaagcg gattacagcg gatatcgttt gtttatcacc ccagagatgg
    20341 aaggctaccc gacagatgaa aatcaagccg ctgcctatat gaacaagatt atcgagaaag
    20401 agatcatgcg cgcaccggag cagtacctct ggatccaccg tcgctttaaa acgcgcccgg
    20461 tgggagaatc gtcgttgtac atttaacaaa ctgctcttac tctttccctc ctcaaatcgg
    20521 ccataataac ctcatggttt ttatggcttt tttcttaagg ttttttcctt cgctgaatat
    20581 ctaattatta ccgtcagaaa ttaaactgtc gccgatatga gacacttgta agttactaaa
    20641 agtaaataag aaaatgcctc ttgtctcgtt tcagttttag gcgtacatta acgccgtctt
    20701 gagcagttta gctcagaatt ctggggtgat gcccacggta aaatacaggg cttttaaggt
    20761 cgctttgccc ggtgtcaact cactgtatca ggtgtaatga agtcattcag gcgtaacagt
    20821 aattacgcgg agagatgtaa agtgaaatat ttctttatgg gcatttcttt tatggtcatc
    20881 gtttgggccg gtacttttgc cctgatgatc taaaagcaaa ctgtgcaatg aaaaacagga
    20941 gccatcaggc tcctgtttgc atttctgacg acggtgttta ttccgcgttt tcgtgaatat
    21001 gtttgctgct ggcgggtagt aaaccgtcgg cacggaacat cgctttaatc cctctgatgg
    21061 cctgacgaat acggtcgcgg ttttcaatca gtgcaaagcg aacatgcgta tcaccatagt
    21121 cgccaaagcc aatccccggc gagacacaaa ccttcgcttc gttaaggagc ttcttggcaa
    21181 attccagcga tcccatggcc gcatatggtt ccgggatttt cgcccagaca tacatcgaag
    21241 ccttcggcat ttcgaccatc cagcccgctt catgcagccc tttaaccaat acatcacggc
    21301 ggcgtttgta ctgttcggta atgtcgcgca cgcactgttg atcgccctcg agtgccgcaa
    21361 tcgccgccac ctgcaacggc gtaaaagtgc cgtaatcgtg atagcttttg attcgcgcca
    21421 gggcactgac cagcgttttg ttgccgacca taaagccgat acgccagccc gccatgttgt
    21481 agcttttcga cagcgtaaag aactcgaccg ccacatcgcg tgcacccggc acctgcataa
    21541 ttgacggcgc tttccagcca tcgtagacga tatcggcata ggccaggtca tggaccacca
    21601 gcacatcgta gcgtttcgcc agcgccacaa ccttttcgaa gaactccagt tccacgcatt
    21661 gcgcggttgg gttcgacggg aagccgagga tcatcatctt cggtttcgga taactttcac
    21721 gaatggcgcg ctccagttcg ttgaagaaat cgacaccttc caccagcggc actgagcgta
    21781 cctgcgcccc ggcaatcacc gcgccataaa tatggatcgg gtaacttgga ttcggcacca
    21841 gcaccgtgtc accatgatcc agcgtcgcca gcatcagatg cgccaggccc tctttcgaac
    21901 caatagtgac gatggcttct gattccgggt cgatttcaac gtcgtagcga tcctgatacc
    21961 agcgggaaat ggcgcgacgt aaccgcggaa tgccgcgtga agtggagtaa ccatgcgtgt
    22021 ccgggcgctg ggccacagta cataattttt cgacgatatg cggcggagtc gcaccgtccg
    22081 ggttacccat gctgaaatcg ataatatctt cgccgcgccg acgcgcagcc attttcagtt
    22141 cagcggtgat gttaaaaacg tagggcggga gacgatcaat gcgcgtaaag cgacgttcag
    22201 ggcgagtgtc agccataatt tcctcagatt aacgttagcg cccggaccgt ccgagcgacg
    22261 ctgccacaat ggtggctgtt ttgaaaatag cctgattaat ttctgtctgt ccagcgtttt
    22321 agaacatttt tatccatggc tttaaatgag gaactccact tcaaaaacag gaaatttttc
    22381 tacgctaaca agaaaattac caccaatttt attaacatta taatttcaat aaatttacca
    22441 gccagaagtg cattatcaag agatacattg ctaatacacc atttatcttt caatcccctt
    22501 ttaaatttat ggtttttcct caaaaacggc ctgctggtca tcagccttca ggttacctat
    22561 catagaggtt taatccttat tcagagtcac ccgtgcacga aatattcaac atgctgctgg
    22621 cggtcttcga tcgggcagcg ttaatgctta tctgcctgtt ctttctcatc cgtatccgcc
    22681 tgtttcgcga actgttgcac aagtcggcgc actccccaaa agaattgctc gccgttaccg
    22741 ccattttctc gctgttcgcc ctgttcagca cctggtccgg cgttcccgta gaaggctcgc
    22801 tggtgaacgt acgtattatc gcggtgatgt ccgacgggat tctgtttggc ccgtgggtag
    22861 gcatcattac cggcgtgatt gcgggtattc accggtattt aattgatatc ggcggcgtga
    22921 cggcgatccc ctgctttatc accagcattc tggcgggttg tatatcaggc tggataaacc
    22981 tgaaaatccc caaagcacag cgctggcgcg tcagtattct cggcggcatg ttgtgtgaga
    23041 cgttgaccat gattctggta attgtctggg caccaactac cgcgttgggg atcgatatcg
    23101 tctctaaaat cggcattcca atgatcctcg gtagcgtctg tatcggcttt attgtgc
//
LOCUS       DADMKF010000056        22878 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.057, whole genome shotgun sequence.
ACCESSION   DADMKF010000056 DADMKF010000000
VERSION     DADMKF010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22878
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.057"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(123..602)
                     /gene="folK"
                     /locus_tag="JNC24_002008"
     CDS             complement(123..602)
                     /gene="folK"
                     /locus_tag="JNC24_002008"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HAY9158755.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESHILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSLAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELAFPDGETLREVLHTRAFDELSKW"
     gene            complement(599..2017)
                     /gene="pcnB"
                     /locus_tag="JNC24_002009"
     CDS             complement(599..2017)
                     /gene="pcnB"
                     /locus_tag="JNC24_002009"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HAY9158756.1"
                     /translation="MTLPRCTIFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQH
                     AISRKDISENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKL
                     FRNCRLVGRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGS
                     IEEDAQRRDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLR
                     AVRFAAKLGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEY
                     HLFQPLFPTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLL
                     ETAQKIAQESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQG
                     KRAWKLLEHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDE
                     EPSPRRRTRRPRKRAPRREGTA"
     gene            complement(2056..2982)
                     /gene="gluQRS"
                     /locus_tag="JNC24_002010"
     CDS             complement(2056..2982)
                     /gene="gluQRS"
                     /locus_tag="JNC24_002010"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414686.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HAY9158757.1"
                     /translation="MLPPYFLFKEMTDTQYIGRFAPSPSGELHFGSLIAALGSYLQAR
                     ARQGRWLVRIEDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHDAYREALAWL
                     HEQGLSYYCTCTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDQLRGII
                     HADEKLAREDFIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQISLYQLF
                     GWKVPDYIHLPLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFS
                     VEQILQSAVKNWTLTAVPESAIVNSTFSNASC"
     gene            complement(3019..3474)
                     /locus_tag="JNC24_002011"
     CDS             complement(3019..3474)
                     /locus_tag="JNC24_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding transcription factor
                     DksA"
                     /protein_id="HAY9158758.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3652..4356)
                     /gene="sfsA"
                     /locus_tag="JNC24_002012"
     CDS             complement(3652..4356)
                     /gene="sfsA"
                     /locus_tag="JNC24_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HAY9158759.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATRGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGAERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMNVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLSVTL"
     gene            complement(4371..4901)
                     /gene="thpR"
                     /locus_tag="JNC24_002013"
     CDS             complement(4371..4901)
                     /gene="thpR"
                     /locus_tag="JNC24_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HAY9158760.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTIPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            4975..7404
                     /gene="hrpB"
                     /locus_tag="JNC24_002014"
     CDS             4975..7404
                     /gene="hrpB"
                     /locus_tag="JNC24_002014"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HAY9158761.1"
                     /translation="MSSLPVAAVLPELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERHYLPLPAHQRFDEAVAVATAEMLRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVLLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFEPRTGLTRLITQRVSQASMTQRAGRAGRLEPGICLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPVVNLMAAKRL
                     LQMLGALDGERLSAQGQKMAALGNDPRLAAMLVSAKNDDEAATAAKIAAILEEPPLMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIAHRRGQDG
                     RYQLANGMGAMLDADDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLAALETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGGVPLVLELLSPAQRPLQITRDLSAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            7600..10134
                     /gene="mrcB"
                     /locus_tag="JNC24_002015"
     CDS             7600..10134
                     /gene="mrcB"
                     /locus_tag="JNC24_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HAY9158762.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDGELYE
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            10354..12543
                     /gene="fhuA"
                     /locus_tag="JNC24_002016"
     CDS             10354..12543
                     /gene="fhuA"
                     /locus_tag="JNC24_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HAY9158763.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAAKHSATATKTDTPIEKTPQSISVVTNEEMQMRQFQSVKEA
                     LGYTPGVTVSSRGASNTYDFVIIRGFSSVGLNQNNYLDGLKLQGNFYNDAVIDPYMLE
                     RVELMRGPTSVLYGKSNPGGIISMVSKRPTTEPLKEIQFKMGTDNLFQTGFDFSDALD
                     DNGEFSYRLTGLARSTNEQQKNSESQRYTIAPSFSWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGASNNTYSRNQKMVGYSFEHGFNDTFTVRQNLR
                     FSEMKTSQKSVYGTGIAGDGHTLNRGTVVDNERLQNFSVDTQLESKFATGDVEHTLLT
                     GVDFMRMRNDINASFGSAPSIDLYNKYHPEYFAFGSAEPYQMNESKQTGIYVQDQAEW
                     NKWVFTLGGRYDWSKQATTVRENSYTPTEGYIERNDHQFTWRGGVNYLFDNGISPYFS
                     YSQSFEPSAFDLWSNPRISYKPSKGEQYEAGVKYVPNDMPVVVTGAVYQLTKTNNLTA
                     DPTNPLAQVPAGEIRARGVELEAKAALNANINLTASYTYTDAEYTKDTNLKGKTPEQV
                     PEHMASLWGDYTFNEGPLSGLTLGTGGRFIGSSYGDPANTFKVGSTAVMDAVVKYDLA
                     RFGMAGSSLAVNVNNLLDREYVASCFQTYGCFWGAERQVVATATFRF"
     gene            12594..13391
                     /gene="fhuC"
                     /locus_tag="JNC24_002017"
     CDS             12594..13391
                     /gene="fhuC"
                     /locus_tag="JNC24_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HAY9158764.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRSGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            13391..14281
                     /gene="fhuD"
                     /locus_tag="JNC24_002018"
     CDS             13391..14281
                     /gene="fhuD"
                     /locus_tag="JNC24_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HAY9158765.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSSEMLARIAPGRGFNFSDGKQPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRAGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            14278..16260
                     /gene="fhuB"
                     /locus_tag="JNC24_002019"
     CDS             14278..16260
                     /gene="fhuB"
                     /locus_tag="JNC24_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HAY9158766.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPGAMASQFAALVGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVAAERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSIGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLYRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(16418..17698)
                     /gene="hemL"
                     /locus_tag="JNC24_002020"
     CDS             complement(16418..17698)
                     /gene="hemL"
                     /locus_tag="JNC24_002020"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HAY9158767.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIIEPVAGNMNCVPPLPD
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYYGVEPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGVHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            17923..19344
                     /gene="clcA"
                     /locus_tag="JNC24_002021"
     CDS             17923..19344
                     /gene="clcA"
                     /locus_tag="JNC24_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HAY9158768.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            19368..19433
                     /locus_tag="JNC24_002022"
     CDS             19368..19433
                     /locus_tag="JNC24_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9158769.1"
                     /translation="MAIIIGLEFAQLPMSFGAKYE"
     gene            19426..19770
                     /gene="erpA"
                     /locus_tag="JNC24_002023"
     CDS             19426..19770
                     /gene="erpA"
                     /locus_tag="JNC24_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HAY9158770.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(19817..20439)
                     /locus_tag="JNC24_002024"
                     /pseudo
     CDS             complement(19817..20439)
                     /locus_tag="JNC24_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414699.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
     gene            complement(20477..21277)
                     /gene="btuF"
                     /locus_tag="JNC24_002025"
     CDS             complement(20477..21277)
                     /gene="btuF"
                     /locus_tag="JNC24_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HAY9158771.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYAQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(21270..21968)
                     /gene="mtnN"
                     /locus_tag="JNC24_002026"
     CDS             complement(21270..21968)
                     /gene="mtnN"
                     /locus_tag="JNC24_002026"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HAY9158772.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            22052..>22878
                     /gene="dgt"
                     /locus_tag="JNC24_002027"
     CDS             22052..>22878
                     /gene="dgt"
                     /locus_tag="JNC24_002027"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HAY9158773.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEQLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVA"
BASE COUNT         5195 a         5805 c         6379 g         5499 t
ORIGIN      
        1 ttgatattta tctgcgggaa tttatattat gtttctgcaa aaatgtttaa actaaaattt
       61 ttattaagga aagagaggat gtatgatttt cagaaaatat ttatattcgt aatataaata
      121 aattaccatt tgcttaattc gtcaaatgct cttgtatgta agacttcacg caacgtctcc
      181 ccatcaggaa acgccagctc cggtgcaatc tcaaacagcg gccacagcat aaatccacga
      241 ttcttcatat cgtagtgcgg aacggtcagg cgttcggtat ttatcacttc attaccaaac
      301 agcatgatat cgagatccag cgtgcgtggt ccccagcgtt cagctttacg aacacgaccc
      361 tgttgcagtt ctatgcgctg tgtatggttg agtaactctt ccggggcgag cgaagtttca
      421 agcgccacgg cggcgttgag gtaatcgggt tgatcttgcg gccccagcgg tggagtgcgg
      481 taaaacgaag aaacggcaag aatgtggctt tcagggatat cgcctaatgc tttcagggca
      541 gcattgacct gctccagcgg agaggccaga ttgctgccta tggcaatata cgccactgtc
      601 atgcggtacc ctcacgacgc ggtgcgcgtt tgcgtggacg acgagtacgg cgacgcggtg
      661 acggctcttc atccaactcg ttaagcatcc ctttttgatc tggtggcgcg gaaacctgga
      721 actcacccca ccatttcacc agacgctgca gttcagcgtt acgttcaact tcagctcgca
      781 aggccaacag gtcataagcc gcacggaact taggatgctc cagcagtttc catgcgcgtt
      841 taccctgacg tcgggacata cgcaactgca actgccagat atcgcgggtt aacgtcgtca
      901 ggcgtttcgg gattgccagt gaacggcagg cttcgtccag cacgtcgttc atcgccagcg
      961 cgaaagcgtc gtgataggtc aggccgcttt cctgggcgat cttctgtgcc gtctccagca
     1021 gtgggtacca gaacatggcg gcaaacaaga acgccgggtt cacgcgcata tcgttatgga
     1081 tacgcgtatc ggtattcttc agcacctgtt caatgatccg ctccatcggg ctgtcgccat
     1141 tttccgtgaa gtagcgggta atggtcggga acagcggctg gaacagatga tattcacaca
     1201 acagcttata ggtttcgtaa ccgtagcccg cttgtagcag tttaagcgat tcttcaaaca
     1261 ggcgtgccgg tgggatatcg ttcagcaggg tagcgaggcg agggatcggt tctgcggttt
     1321 ccgggctgat gcgcataccc aatttggcgg caaaacgtac cgcgcgcagc atccgtaccg
     1381 gatcttcacg gtagcgcgtt tccgggttac caatcagacg gataacgccg tccttcagat
     1441 ccttcatacc gccaacgtaa tcacggacgg taaaatccgc tacgctgtaa tagaggctgt
     1501 tgatagtgaa atcgcggcgc tgggcgtctt cttcgatgga gccgaaaatg ttgtcgcgca
     1561 gcaacatgcc gttttgcccg cgttgggagg tcgtgcggtc gctgacgtta ccttcgtggt
     1621 gtccacggaa ggtcgcaact tcgataatct ccgggccaaa cattacatga gccagacgga
     1681 aacggcgacc caccaggcgg cagttacgga acagtttgcg cacctgctca ggcgtggcgt
     1741 tagtggttac gtcaaaatct ttcggctttt tgccaagtaa caggtcgcgc acgccgccgc
     1801 caaccagcca ggcttcgtat cccgctttgt tgagcctgta cattaccttc agggcatttt
     1861 cactgatatc tttgcgggaa atagcatgct gctcacgcgg gatcaccgtc acctgtggac
     1921 gggcgactgc ctgttcagcc tcgctttcct cgcggcttag caccttgcgg caaaaattag
     1981 cgactcgggt aaaaatagta cacctcggta gtgtcaaaca tcattcagga caaaaaaata
     2041 gcggctaatc atagctcagc atgacgcatt tgagaatgtt gaatttacaa ttgccgactc
     2101 gggcacggcg gtcagcgtcc agtttttgac tgctgactga aggatttgtt cgacgctgaa
     2161 atcctgccag tgtgcttctg cctgctgacc cagaaattga agtgccgcga ttagtaccgg
     2221 acgtggatcg cctttcggca acgcaggcgc atgattctgc ttggaaagtt tagcgccttg
     2281 tggattaagc gccagcggca gatgaatgta atctggcact ttccagccaa aaagctggta
     2341 cagcgagatt tgccttactg ttggttcaat cagatcagcc ccacgcacta tttctgtaac
     2401 gccctggaaa tgatcatcaa ccacaacagc caggttgtag gcgaacaacc catcacggcg
     2461 atgaatgata aaatcttccc gtgccagttt ttcgtcggcg tgaataatgc cgcgcagctg
     2521 gtcagtaaat tgcgtgaccg gatgctgctg gcggatacgc actgcggcat tgtctggtcc
     2581 atgatgcaac acccggcaat gaccgtcgta aataccgcca atgctttgaa tacgcgcacg
     2641 cgtacaggtg cagtaataac ttagtccttg ttcatgtaac caggcgagtg cttcacgata
     2701 ggcgtcgtga cgttgcgatt gccagagaac atcgccgtcc cagtgcagac cgtaatgttc
     2761 cagctggcgc aggatagttt ctgcggcacc gggaacttca cgaggcgggt cgatatcttc
     2821 tatgcgtacc agccagcgac cttgccgggc gcgagcctgc aaatagctgc cgagcgcggc
     2881 gatcagagag ccaaaatgaa gctcgccgga aggagagggg gcgaagcggc caatatactg
     2941 tgtgtctgtc atctctttga acaaaaaata aggcgggagc atttcccgcc tgtggtaaac
     3001 gtgatggaac ggctgtaatt agccagccat ctgtttttcg cgaatttcag ccagcgtttt
     3061 gcagtcgatg cacagatcgg ctgtcgggcg cgcttccaga cggcgaatac caatttcaac
     3121 accgcaggat tcgcagtagc cgaaatcttc gtcttccact tttttcagcg tcttctcgat
     3181 ctttttgatc agcttacgct cgcgatcgcg gttacgcagt tcgaggctga actcttcttc
     3241 ctgggctgca cggtctaccg ggtccgggaa gttggctgct tcatcctgca tatgtgtaac
     3301 ggtgcgatcg acttcatccc tgagttgatt acgccatgct tccagaatac gacggaagtg
     3361 cgccagctgg gcttcattca tatactcttc gcccggcttc tcctgatatg gttccacccc
     3421 agcgatggcg agaatactca gggacgatgt tttacggttt tgcccttctt gcatgttgct
     3481 tctccttaac acgcactatc gatccccatg ttcgggggaa aaatgaggcc gctataaata
     3541 gcagatgctt ttccggatag caattatcta aacgtaacac ttgacaactg tgtgaggaaa
     3601 agcgtatttg cgcacgcgac cagaatgtaa attaaccagt tacttacttt actacaatgt
     3661 aaccgacagt gattttttaa gagccatgcc ttcagcagaa atttccgctt tgtaagccag
     3721 aatttctacc cccctctgtt gagcttctga caatagttgc gcgtatttct catcgatgtg
     3781 gcgcgcgggt gaaaaccgtg taatggctga atgcagcacg gcgaaaaaga taaccgcacg
     3841 ctggccttca gccgctacgt tcatcaactc ccgaaggtgt ttctgacctc gttcagtgac
     3901 cgcatcggga aaatatccct gttcgttctc cgctaacgta accgatttca cttcaatata
     3961 gcagtctgga cgcgaatccg cctgcaacat aaagtcaata cggctgcgtt ctgcgccgta
     4021 ttttacttcg cttttcagcg agctatagcc tgacagttct gaaattgatt cattaaggat
     4081 agcctctttc gtcaacctgt tagcccaaag cgtattgacg caaataaatg cgccgctctg
     4141 gctttgagtt agttcccagg tgtgtgggta tttccgtttg gtgttgtctg aagtcgaata
     4201 ccagacggta tcgccacgcg ttgcacaacc ggtcatcgca cccgtattcg ggcagtgtag
     4261 cgttaattcg cgaccatcgg gtgtgatcac atcggctaaa aaacgtttgt aacgctgaat
     4321 tagcgtcgcg cgctgtagag ggggagagaa ttccattgcg acaatccttg ttattgcgtt
     4381 agcgcccagc gttttagcgg cgtgtagcgt gtgcgtccac gggcaaacga ggaggcgtaa
     4441 agggtgaact ccgtcaccgc ataagaccag ttaaaacctg gcggcgggat tgtcaccgcc
     4501 tcgctggcgt cgcgcaataa ggtaatatgt ggatgaaacg gacgattgct ttgaaaacaa
     4561 ccgctgcggg cagcctgtga acggagcata ttcgccagct ggattaagcc gcgtggcggc
     4621 tgacgcatcc ctaaccacac cacacgcgaa cgcagccatt gtccggcgtc atcaagcgtg
     4681 agtgtgaaac caggttgacg aatccgtccg gctaaaagag aaagcgcctt ctctttctcg
     4741 gcgctcactt cgcctaaaaa tgccagagtc agatgcaaat tatcggcggc gaccggacgt
     4801 cccgcctcag gtgggaagtg tgtggcgcgc cagtggataa tctgttcgcg gatttctgca
     4861 ggtaagtcga tagcaaagaa cagacgttgc ggttcagaca tgtgaggcac tcggttatga
     4921 attaccgcga tgctacaatg tggcgcgaag aatgttaacc ctctggagcg ttttgtgtcg
     4981 tcgttgcccg ttgctgccgt cttacctgaa ttacttaccg ctctcgattg tgcgccgcag
     5041 gtattattaa gtgcgccgac cggggccggg aaatcaacct ggctgccgct gcaactgctg
     5101 gcgcatcccg gcattaacgg gaaaattatc ctgctggagc cgcgtcgtct ggcggcacgt
     5161 aacgtcgcgc aacggctggc ggagctgctt aacgaaaagc caggcgatac cgttggctac
     5221 cggatgcgtg cgcaaaactg cgtcgggcca aatacccgtc tggaagtagt taccgaaggc
     5281 gtgctgacgc ggatgatcca gcgtgacccg gagttgagcg gtgttggatt ggtgatcctt
     5341 gatgaatttc atgagcgcag cttgcaggcg gatttggcgt tggcgctgtt actcgatgtg
     5401 caacaaggtc tgcgtgatga ccttaaactg ctgattatgt cggctacgct ggataacgac
     5461 cgcttgcagc aaatgctgcc agaagcgccc gtcgtcatct cagaagggcg ctcgtttccg
     5521 gttgaacgcc attatttacc gctgcccgcg catcagcgtt ttgacgaagc cgttgcggta
     5581 gccaccgccg aaatgctgcg tcaggaaagc ggatcattac tgttattttt acctggcgtc
     5641 ggagaaattc agcgtgtgca ggaacaactg gcttcgcgca tcggcagtga tgtgttgctc
     5701 tgcccgctgt atggcgcgtt gtcgctgaac gatcagcgaa aagcgatcct cccggcaccg
     5761 caagggatgc gcaaagtggt gctggcgacc aatattgctg aaaccagttt aaccattgaa
     5821 ggtattcgtc tggtggtgga ttgtgcccag gagcgtgtgg cgcgttttga gccgcgcacg
     5881 gggcttacgc gactgattac tcaacgcgtt agccaggcat ccatgacgca gcgtgccggg
     5941 cgcgccgggc gtctggagcc gggtatctgc ctgcatttaa tcgccaaaga acaagcagaa
     6001 cgcgctgccg cgcaaagtga accggagatc ttacaaagcg atctttccgg tttgctgatg
     6061 gaattactgc aatggggatg cagcgatccg gcgcagatga gctggctgga tcaaccgcca
     6121 gtagtgaatc taatggccgc gaaacgtctg ttacaaatgc tgggggcgct ggacggtgaa
     6181 cggcttagtg cgcaagggca aaaaatggca gcgctgggta acgatccgcg tttagcggca
     6241 atgctggtta gcgcgaagaa cgacgacgaa gctgctaccg cggcaaaaat tgccgccatt
     6301 ctcgaagagc cgccactgat gggcaatagt gacctgggcg tggcgttttc gcgcaatcaa
     6361 ccagcctggc agcaacgtag tcagcaactg ttaaaacgct taaacgtacg cggcggtgag
     6421 gcagacagtt cgcttatcgc gccgctactt gccggggcgt ttgccgatcg cattgctcat
     6481 cgccgtgggc aagatggacg ctatcaactg gcaaacggca tgggagcgat gctcgatgcc
     6541 gacgacgcgc taagccgcca cgaatggttg atcgcaccgt tattattgca gggcagcgcc
     6601 tcgccggatg cgcggatttt actggcgcta ccggtcgata ttgatgaatt agtacaacgc
     6661 tgtccgcagc tggtgcagca atctgacacc gtggaatggg atgacgcgca aggtacgctg
     6721 aaagcctggc gtcggctaca aatcggtcag ttgacggtga aagtgcagcc gctggcgaaa
     6781 ccgtcagaag acgagttgca tcaggcgatg cttaatggca tccgtgataa aggtttaagc
     6841 gtgctcaact ggacggcgga agcggaacag ctacgcttgc gtttgttatg cgccgcaaag
     6901 tggttgccgg aatatgactg gccagcggtt gatgatgaaa gtttattggc agcgctggaa
     6961 acgtggctgc tgccacatat gacaggcgta cattcactac gcggcctgaa atcactggac
     7021 atttatcagg cactacgcgg attacttgat tggggaatgc agcaacgtct ggatagtgaa
     7081 ttgcctgcgc attacactgt gccgacggga agccggatcg ccattcgtta tcatgaagat
     7141 aacccgcccg cactggcggt gagaatgcag gagatgtttg gtgaggccac taatccgact
     7201 atcgcccagg ggggcgtgcc gctggtgctg gagttgcttt cacccgccca aaggccacta
     7261 caaatcacac gagatttgag cgccttctgg aaaggagcgt accgtgaggt gcaaaaagag
     7321 atgaaagggc gttatcccaa acacgtctgg ccggacgacc cggcaaacac ggcaccgacg
     7381 cgacggacga aaaagtattc gtaaggttgg ttttctccct ctccctgtgg gagagggccg
     7441 gggtgagggc atcagcgcgc acgttcatcc tcaccctaac cctctccctc aagggagagg
     7501 ggaccgatcg agcacaaatt ttgagagata tcttcttctg tcttgtaaca gaagaacaga
     7561 aaatcgggct tttgcgcctg aatattgcgg agaaaaagca tggccgggaa tgaccgcgag
     7621 ccaattggac gcaaagggaa accgacgcgt ccggtcaaac aaaaggtaag ccgtcgtcgt
     7681 tacgaagatg acgatgatta cgacgattat gatgactatg aggatgaaga accgatgccg
     7741 cgcaaaggta agggcaaagg caaagggcgt aagcctcgtg gcaaacgcgg ctggctatgg
     7801 ctgctgttaa aactggctat cgtttttgcc gtgctgatcg ccatttacgg cgtttatctc
     7861 gatcaaaaaa ttcgtagccg tattgatggc aaggtctggc aactgcccgc ggcagtttat
     7921 ggccgaatgg tcaaccttga accagatatg accatcagca agaacgaaat ggtgaagctg
     7981 ctggaggcaa cacagtatcg tcaggtgtcg aaaatgaccc gtcctggcga atttaccgtg
     8041 caggccaaca gcattgagat gatccgccgt ccgtttgatt tcccggacag caaagaaggg
     8101 caggtgcgtg cgcgtctgac ctttgatggc gatcatctgg cgacgatcgt caatatggag
     8161 aacaaccgtc agttcggttt cttccgtctt gatccgcgcc tgatcaccat gatctcttcg
     8221 ccaaacggtg agcagcgtct gtttgtgccg cgcagtggtt tcccggactt gctggtggat
     8281 actttgctgg cgacagaaga ccgtcatttc tacgagcatg atgggatcag tctctactcc
     8341 atcggacgtg cggtgctggc aaacctgacc gccggacgca cggtgcaggg agcgagtacg
     8401 ctgacgcaac agctggtgaa aaacctgttc ctctccagcg agcgttctta ctggcgtaaa
     8461 gcgaacgaag cttacatggc gctgatcatg gacgcgcgtt acagcaaaga ccgtattctt
     8521 gagctgtata tgaacgaggt gtatctcggt cagagcggcg acaacgaaat ccgcggcttc
     8581 ccgcttgcga gcttgtatta ctttggtcgc ccggtagaag agctcagcct cgaccagcag
     8641 gcgctgttag tcggtatggt gaaaggggcg tccatctaca acccgtggcg taacccgaaa
     8701 ctggcgctgg agcgacgtaa tctggtgctg cgtttgctac aacaacagca gattatcgat
     8761 ggtgaactgt atgaaatgct cagcgcgcgt cctctgggcg tacagccgcg cggtggggta
     8821 atctctccac agccagcctt tatgcaactg gtgcgtcagg agctgcaggc gaaattaggc
     8881 gataaggtaa aagatctctc cggcgtgaag atcttcacta cctttgactc ggtggcccag
     8941 gacgcggcag aaaaagccgc cgtggaaggc attccggcac tgaagaaaca gcgtaagttg
     9001 agcgatcttg aaactgcaat tgtggtcgtc gaccgcttta gtggtgaagt tcgtgcgatg
     9061 gtgggaggtt ctgagccgca gtttgcgggc tacaaccgtg cgatgcaggc gcgtcgttcg
     9121 attggttccc ttgcaaaacc agcgacttat ctgacggctt taagccagcc gaaaatctat
     9181 cgcctgaaca cgtggattgc cgatgcgcca atcgcgctgc gtcagccgaa tggccaggtc
     9241 tggtcaccgc agaacgatga ccgtcgttat agcgaaagcg gcagagtgat gctggtggat
     9301 gcgttgaccc gttcgatgaa cgtgccgacg gtaaatctgg ggatggcgct ggggctgcct
     9361 gcagttacgg agacctggat caaactgggc gtgccgaaag atcagctgca tccggttccg
     9421 gcgatgctgc tgggggcgtt gaacttaacg ccaatcgaag tggcgcaggc attccagacc
     9481 atcgccagcg gcggtaaccg tgcaccgctt tctgcactgc gttcggtaat cgcggaagat
     9541 ggcaaggtgc tgtatcagag cttcccgcag gcagaacgcg ctgttccggc gcaggcggcg
     9601 tatctgacac tatggaccat gcagcaggtg gtacaacgtg gtacgggtcg tcaacttggg
     9661 gcgaaatacc cgaacctgca tctggcaggg aaaacaggga ctaccaacaa taacgtagat
     9721 acctggtttg cgggcattga cggcagcacg gtgaccatca cctgggtagg ccgtgataac
     9781 aaccagccga ccaaactgta tggtgccagc ggggcaatgt ctatttatca gcgttatctg
     9841 gctaaccaga cgccaacgcc gctgaatctt gttccgccag aagatattgc agatatgggc
     9901 gtggactacg acggcaactt tgtttgcagc ggtggcatgc gtgtcttgcc ggtctggacc
     9961 agcgatccgc aatcgctgtg ccagcagagc gagatgcagc agcagccgtc aggcaatccg
    10021 tttgatcagt cttctcagcc gcagcaacag ccgcaacagc aacctgctca gcaagagcag
    10081 aaagacagcg acggtgtagc cggttggatc aaggatatgt ttggtagtaa ttaacatcta
    10141 agcgtgaaat accggatggc gaattgccat ccggtaaaat aacatcccac ctaaagtatt
    10201 aaccctttct tttcatctgg ttgtttatta acccttcagg aacgctcaga ttgcgtaccg
    10261 cttgcgaacc cgccagcgtt tcgaatatta tcttatcttt ataataatca ttctcgttta
    10321 cgttatcatt cactttacat cagagatata ccaatggcgc gttccaaaac tgctcagcca
    10381 aaacactcac tgcgtaaaat cgcagttgta gtagccacag cggttagcgg catgtctgtt
    10441 tatgcacagg cagcggttga accgaaagaa gacactatca ccgttaccgc tgcacctgcg
    10501 ccgcaagaaa gcgcatgggg gcctgctgca actattgcgg cgaagcactc tgctactgcg
    10561 actaaaacgg atacaccaat tgaaaaaacg ccgcagtcta tttcggttgt gacaaatgaa
    10621 gagatgcaga tgcgtcaatt tcagtctgta aaagaagcat taggttatac acctggcgtt
    10681 acggttagca gtcgtggtgc ttctaataca tatgattttg ttatcattcg tggtttctca
    10741 tctgttggtc tgaatcaaaa taattacctt gatggactaa aacttcaggg caacttttat
    10801 aacgatgctg tgattgatcc ttacatgctc gagagggttg aactgatgcg tgggccgacg
    10861 tctgttctct atggaaaaag taatcctggt gggattattt ctatggtgag taagcgtccg
    10921 accactgaac ccctgaaaga aattcagttt aaaatgggga cggataatct gtttcagacc
    10981 ggattcgatt tcagtgatgc actggatgat aacggtgagt tctcttaccg tttgacgggc
    11041 ctcgctcgct caacaaacga acagcagaaa aactctgaat ctcagcgtta taccattgct
    11101 ccatcattct catggcgtcc agacgacaaa accaatttta ctttcctgtc ctatttccag
    11161 aatgaaccag aaacgggtta ttacggttgg ttgccgaaag aggggaccgt tgagccattg
    11221 cctaatggta agcgtctacc gactgacttc aatgaaggtg catcgaataa tacatactcc
    11281 cgtaaccaga aaatggtggg atatagtttt gaacatggtt ttaatgacac cttcaccgtg
    11341 cgtcagaatc tgcgtttcag tgaaatgaaa acctcacaga aaagtgttta tggcacaggg
    11401 attgctggtg atggtcatac cctaaaccgc gggacagtgg tggataatga gcgtctacaa
    11461 aactttagcg ttgataccca acttgaaagt aagtttgcta caggtgacgt cgagcatact
    11521 ttgctgacag gggtagactt catgcggatg cgtaatgata tcaatgccag ctttggatcc
    11581 gcaccatcca tcgatcttta taacaaatat caccctgaat actttgcatt tggtagcgca
    11641 gagccatacc agatgaatga aagcaaacaa acaggtattt atgttcagga tcaggcggaa
    11701 tggaataaat gggtattcac tctgggggga cgctacgatt ggtctaagca agcgaccact
    11761 gttcgtgaaa actcttatac gccgactgaa ggttatattg agcgcaatga tcatcagttc
    11821 acctggcgcg gtggtgtaaa ttacttattc gataatggta tttcacctta ctttagctat
    11881 agccagtcct ttgaaccgag tgctttcgat ctgtggagca acccgcgcat ttcctataag
    11941 ccatcgaaag gtgaacagta tgaagctggc gtaaaatatg ttccgaatga tatgccggtc
    12001 gttgttacgg gcgcagtcta tcaattgacg aaaacaaata acctgacagc agacccaaca
    12061 aacccattag cgcaagtccc agcaggtgag attcgcgctc gtggtgtgga acttgaagca
    12121 aaagcggcgt taaatgccaa tattaacttg acggcttctt atacctacac cgatgcggaa
    12181 tacaccaaag acaccaatct caaaggtaaa actccagaac aagtaccgga gcatatggca
    12241 tctctctggg gggattatac cttcaatgaa gggccgcttt ctggtttaac attgggaaca
    12301 ggtggtcgtt ttattggttc cagctatggt gatccggcaa acacttttaa agtgggtagc
    12361 acagctgtaa tggatgctgt tgtaaaatat gatctggcac gctttggtat ggcgggatcc
    12421 agccttgctg tgaacgtcaa caatttgctc gatcgtgagt atgttgccag ttgcttccag
    12481 acttatggct gcttctgggg cgcagaacgt caggtcgttg caaccgcaac cttccgtttc
    12541 taatttctct tttggggcac ggatttccgt gcccatttca caagttggct gttatgcagg
    12601 aatacacgaa tcattccgat accacttttg cactgcgtaa tatctccttt cgtgtgcccg
    12661 ggcgcacgct tttgcatccg ctgtcgttaa cctttcctgc cgggaaagtg accggtctga
    12721 ttggtcacaa cggttctggt aaatccactc tgctcaaaat gcttggtcgt catcagcctc
    12781 cgtcagaagg ggagattctt cttgatgccc agccgctgga aagctggagc agcaaagcgt
    12841 ttgcccgcaa agtggcttat ttgccgcagc agcttcctcc ggcagaaggg atgaccgtgc
    12901 gtgaactggt ggcgattggt cgttacccgt ggcatggcgc gctggggcgc tttggtgcgg
    12961 cagatcgcga aaaggtcgag gaagctatct cgctggttgg cttaaaaccg ctggcgcatc
    13021 ggctggtcga tagtctctct ggcggcgaac gtcagcgggc gtggatcgcc atgctggtgg
    13081 cgcaggatag ccgttgtctg ttgctcgacg aaccgacttc ggcgctggat atcgcccacc
    13141 aggttgatgt gctggcgctg gtgcaccgtt taagtcagga acgtggcctg acggtcattg
    13201 ccgtgttgca cgatatcaat atggcggcac gctactgtga ttatctggtc gccctgcgca
    13261 gtggtgaaat gattgctcag ggaacgcctg cggaaattat gcgcggcgaa accctcgaaa
    13321 tgatttatgg catcccgatg ggtattttgc cgcatccggc gggtgctgca cctgtgagtt
    13381 ttgtttattg atgagcggct tacctcttat ttcgcgccgt cgactgttaa cggcgatggc
    13441 gctttctccg ttgttatggc agatgaatac cgcccacgcg gcggctattg accccaatcg
    13501 tattgtggcg ctggagtggt tgccggtgga attactgctg gcgctcggca tcgtgcctta
    13561 cggcgtggcg gataccatca actatcgcct gtgggtcagc gaaccaccat tgccggactc
    13621 agtgattgac gtcggtttgc gtacagaacc taaccttgaa ctgctgaccg aaatgaaacc
    13681 gtcgtttatg gtctggtcgg caggatatgg cccttcatca gaaatgctgg cacgtattgc
    13741 gccgggtcgc ggatttaact tcagtgacgg caaacagccg ttggcgatgg cgcgtaaatc
    13801 gctgacggaa atggcagatt tacttaacct gcaaagcgca gcggaaacgc atttagcgca
    13861 atacgaagac tttatccgca gcatgaaacc ccgctttgtg aagcgtggtg cacgcccgtt
    13921 actgctgacg acgcttatcg atccgcgcca tatgctggtc ttcggtccaa acagcttgtt
    13981 ccaggaaatt ctcgatgagt acggcatccc aaatgcctgg caaggggaaa ccaacttctg
    14041 gggcagtacc gccgtcagta tcgatcgtct ggcagcgtat aaagacgttg atgtgctctg
    14101 ttttgatcac gacaacagca aagacatgga tgcgctaatg gcaaccccgc tgtggcaggc
    14161 catgccgttt gtccgcgccg gacgctttca gcgcgtacct gcagtctggt tttatggtgc
    14221 gacactctcg gcaatgcact ttgtgcgcgt tctggataac gccatcggag gtaaagcgtg
    14281 agtaaacgaa ttgcgctttt tccggcgtta ttgctggcgc tgttagtgat tgtcgctacg
    14341 gcgctcacct ggatgaactt ctcgcaggcg ctgccgcgta gccagtgggc gcaggctgcc
    14401 tggtcgccag acatcgatgt catcgagcag atgatttttc actacagctt gttgccgcgt
    14461 ctggcgattt cgctgctggt gggcgcgggc ctggggctgg tgggcgtgct gtttcagcaa
    14521 gtgctgcgta acccgctggc ggagccgacg acgcttggcg ttgctacagg cgcgcaactg
    14581 ggaattaccg tcaccacact ttgggcgatc ccaggtgcta tggcgagcca gtttgccgca
    14641 ctggtgggcg cttgtgtcgt tggcttaatc gtctttggcg tcgcgtgggg gaaacggctt
    14701 tcgccggtta cgcttattct cgcggggctg gtggtgagcc tttattgcgg ggcaattaat
    14761 cagctactgg ttatcttcca tcacgaccag ctgcaaagca tgttcctgtg gagcaccgga
    14821 acgctgacgc aaaccgactg gggcggcgtt gagcgtttat ggccgcagtt gctgggcggc
    14881 gtgatgttga cgttattgct acttcgcccg ttaaccctga tggggcttga tgatggcgtg
    14941 gcgcgcaatc tcgggctggc cttgtcgctc gcgcgtctgg cggcgctgtc gctggcgata
    15001 gtcatcagtg cgctgctggt gaatgcggtg gggattatcg gctttatcgg tttgttcgcg
    15061 ccgctgctgg cgaaaatgct gggggcgcgg cgtttgttgc cacggttgat gctggcgtcg
    15121 ctgattgggg cgctgatcct ctggctttcc gatcaaatca tcctctggct gactcgcgtg
    15181 tggatggaag tgtccaccgg ttcggtcact gcgttgatcg gtgcgccgct gctactgtgg
    15241 ctgttgccgc gtttacgcag cattagcgcg ccggatatga aggtcaacga tcgtgtcgcg
    15301 gctgaacgcc aacatgtgct ggcgtttgcc ctcgcgggcg gcgtgctgct gttgatggct
    15361 gtggtggtgg cgctgtcgtt tggtcgtgat gcgcacggct ggacgtgggc gagcggggcg
    15421 ttgctcgagg atttgatgcc ctggcgctgg ccgcgaatta tggcggcgct gtttgcgggc
    15481 gtcatgctgg cggtggcggg ctgtattatt cagcggctga ccggaaaccc gatggcaagc
    15541 ccggaagtgc tggggattag ctctggcgcg gcgtttggcg tggtgttaat gctgtttctg
    15601 gtgccgggta atgcctttgg ctggctgtta cccgcgggca gtatcggggc ggcggtgacg
    15661 ctgttgatca ttatgatcgc cgccggccgc ggtggatttt ccccacaccg tatgttactg
    15721 gcggggatgg cgttaagtac cgcattcacc atgcttttga tgatgttgca ggcgagcggt
    15781 gacccgcgaa tggcgcaagt gctgacctgg atttccggtt cgacctacaa cgcgaccgat
    15841 gcgcaggtct ggcgtaccgg aattgtgatg gtgattttgc tggcgattac cccgctgtac
    15901 cgccgctggc tgaccatttt accgctgggt ggtgataccg cacgagccgt aggaatggcg
    15961 ctgacgccga cgcgaattgc gctgctgctg ttagcggctt gcctgacggc gaccgcgacg
    16021 atgactattg gaccgttgag ttttgttggt ttaatggcac cgcatattgc gcggatgatg
    16081 ggctttcgac ggacgatgcc acacatcgta atttcggcgc tggtgggtgg tttactgctg
    16141 gtattcgctg actggtgtgg gcggatggtg ctgtttccat tccagatccc ggcggggctg
    16201 ctgtcgacct ttatcggcgc gccatatttt atctatttgc tgagaaagca gagccgttaa
    16261 tttttccata taacggccct gtacgcctgg acggataaag cgtttacgcg acaacggatg
    16321 cccgatgcga cgctggcgcg tcttatcggg cctacaaacg gccccgaatc gtaggtcgga
    16381 taaggcgttc acgccgcatc cgacaaacca tgctggatca caacttcgca aacacccgac
    16441 gtgcagcatc gatggtgtta ttgatatctt ccatgctgtg cgccacggac ataaagcccg
    16501 cttcaaacgc tgacggtgcc aggtaaacac cttcgtccag catcatatgg aagaaacgct
    16561 taaagcgttc cacgtcacag gccatcacat cctgatagca cgtcacggac tcggcgtcgg
    16621 taaagaaaat accgaacatg ccgccaacgt ggttaacgac cagcggaatt ccggcttctt
    16681 ctgccgcttc cagcagacct tctgccagac gtgttgtcag ctcatccaac gtttcgtgaa
    16741 cgcccggttg cgcgacttca ttcagacagg cgaaaccggc tgccatcgca attgggttac
    16801 cggaaagcgt acccgcctga tagaccggac ccgtcggggc cagcgcatcc attacatcac
    16861 gacgaccacc gaatgcgcct accggcattc caccgccgat gattttgccc aggcaggtga
    16921 gatccggttc cacaccgtaa taatcctgtg cgccagccag tgccacgcgg aagccggtca
    16981 ttacttcatc gatgatcagc aatgcgccaa attcgtcgca cagcgcacgc agacctggca
    17041 ggaagtctgg cagcggtgga acacagttca tattgcctgc caccggctcg atgataatac
    17101 aggcaatctc ttgcgggtat tgctcaaacg cggcgcgtac agaagccaga tcgttataag
    17161 tacaggttaa ggtatgtttg gcgaaatctg ccggaacgcc cggcgagttt ggctggccta
    17221 acgtgagtgc gccagaaccg gctttcacca gcaggcagtc agcgtgaccg tggtaacacc
    17281 cttcaaattt aatgattttg tcgcgaccgg taaaaccacg ggccagacgg atggcgctca
    17341 tcgtcgcctc ggtgccggag ttcaccatgc gcaccatatc catggtcggg accaattcgg
    17401 tcaccagttg cgccattttc acttccattt cggttggtgc accaaagctt aaaccacgct
    17461 cggcggcttc aatcacggca ttgcggattg ccggatggtt atggcccagc accatcggcc
    17521 cccaggaacc gacataatcg atataggctt tgccatcaac atcgtacaga taagcgccgt
    17581 ccgctttttc gataaacagt ggagtgccgc ccacgccagt aaaggcgcga acaggggagt
    17641 tcacaccgcc agggatcagc tcgcgcgctg cgctgtaaag attttcagac ttactcatgg
    17701 agggttcctg attcgtagaa aaagtgaatg gctgctattc tatgttattc ataacaagtt
    17761 aaatactcgt caaacatcag gctgcttgta ctggtgcaat cctgatttct tagagtataa
    17821 aagttttgtg catttgaaac attacgcttt gcaaaggatt ttcatggaac gtgcgagtaa
    17881 aatgccgtca tcttatttgt atgaccaata agtgatcatt ggatgaaaac tgatactccc
    17941 tctttagaaa caccgcaggc cgcgcgcctg cgacgcagac aactgattcg ccaacttctt
    18001 gagcgcgata aaaccccgtt agccattttg tttatggcgg cagtcgtcgg cacgcttgtt
    18061 gggctggcag cggttgcttt tgacaaaggt gtcgcctggt tgcagaacca acgtatgggg
    18121 gcgctggtac atactgctga taattatccg cttctgttaa ccgtcgcttt tctctgttcg
    18181 gcggtgctgg cgatgtttgg ctactttttg gtgcgcaaat acgcgccgga agcaggtggt
    18241 tcggggatcc cggaaattga aggggcgctg gaagatcaac gtcccgttcg ctggtggcgt
    18301 gtattgccgg tgaagttctt tggcgggctg gggacactcg gcggaggcat ggtgttgggg
    18361 cgcgaagggc caaccgtgca gatcggcggt aacattggcc gtatggtgct tgatattttc
    18421 cgcctgaaag gtgacgaagc tcgccatacg ctgctggcaa ccggtgctgc tgcggggctg
    18481 gctgcggcct ttaacgcgcc gctggcgggt attttgttta ttatcgaaga gatgcgtccg
    18541 cagtttcgct atacgttaat ttcgataaaa gcggtattta ttggcgtcat tatgtcgacc
    18601 attatgtacc ggatttttaa tcatgaagtt gcgttgattg acgtcggtaa actttctgat
    18661 gcgccgctta atacgctgtg gctttatctg atcctcggta ttatttttgg cattttcggc
    18721 cctattttta ataaatgggt gctggggatg caggatttgc tgcaccgtgt gcacggcggc
    18781 aatattacca aatgggtact gatgggcggt gcgattggcg gtctgtgtgg attgctgggg
    18841 tttgtggcac cagcaacgtc gggcggcggt tttaacctga ttcctatcgc taccgcgggg
    18901 aatttcagca tgggaatgct ggtgtttatc ttcgtcgctc gggtcattac caccttactc
    18961 tgcttctctt ccggcgcgcc gggcggtatt tttgccccga tgctggcgct gggtactgtg
    19021 ctgggaaccg ctttcggaat ggttgccgtt gagctgtttc cgcaatatca ccttgaggcg
    19081 gggacgtttg ctattgccgg aatgggggca ttactggcgg catctattcg cgcgccgtta
    19141 acggggatca ttctggttct ggagatgacc gataactacc agctcatttt gccaatgatt
    19201 attaccggtc ttggcgcaac actattagcg caatttaccg gcgggaaacc gctatactcg
    19261 gcgattcttg cgcgcacgct ggcaaaacag gaagctgagc aactggcgcg aagcaaggcc
    19321 gcatcagcca gcgagaatac ttgaacgaaa taccagggta ttagataatg gcgattatta
    19381 ttgggttaga atttgcccaa ttgccgatgt cgtttggagc aaaatatgag tgatgacgta
    19441 gcactgccgc tggagtttac cgacgcagca gccaacaaag ttaaaagcct gatcgctgac
    19501 gaagataacc cgaatctgaa attacgcgtg tatatcaccg gtggcggttg cagcggcttc
    19561 cagtatggtt tcacctttga tgatcaggtg aacgaaggcg atatgaccat cgaaaaacag
    19621 ggcgttggct tggtggttga tccgatgagc ctgcaatatc tggtcggcgg ttccgttgac
    19681 tataccgaag gactggaagg ttctcgtttc atcgtgacca acccgaacgc gaaaagcacc
    19741 tgcggttgcg gttcttcctt tagcatctaa tctgttgttc atgtgccgga taaactatcc
    19801 ggcgcatctt ctctcttcaa cgcccattct catccagcgc aaacgtcggt agcttaagat
    19861 gccaacgaat agccgccagc cgaatcaata gcgtcacgac catgcccatc atactggctg
    19921 tttccagtgg tacggaaaat gtgtaataag ccgtagcgtg gacaataccg ccgataatac
    19981 aggcagttgc gtagatttct gtacgtaaaa tcatggggat ctcgcgggcc agtacatcac
    20041 gaatgatccc gccgccaacg ccagtaatga cgaccataca aaccgcgatt aacggaccga
    20101 cttccgcatt aaaggcttta ttcacgccaa tgccgacaaa caccgccaga ccaacggcgt
    20161 ccagcaccgg caacatcctt ttggtaagcg tcttggctgg cgcaccagca cgatggtcag
    20221 catgctggtt accattgcaa cgaccagatc ggtgggatct ttcacccaaa ataccgggcc
    20281 gtgatccagc gccatgtcgc gaattgtccc gccgcctaca gcggtaacca cgcccagtac
    20341 cagaacacca aaagggtcca tacgcaattt tccggctaac aaaacgccgg agatggcaaa
    20401 taccgctgtg ccgactatat ccagccaata gacgagcatt gttcaatccc cactgagcac
    20461 ctaaaaaggt caggcgctaa tctacctgtg aaagcgcatt acagagctgt tgtgcagcga
    20521 ggataatacg tgggcttgca cgttcaaacc agtcactcgt gagaggaata acgggaattt
    20581 tgagctgttc accccagtat tgtttgattt taggaatttg gtccggtccg cctgtaatga
    20641 caatcgcctg tggcgagcgt gctaacacct gttcgcggct aacttgcggc cagggaaccc
    20701 ggctgtcttt aaagatgttt tctccgccac aaacttcgag tacctggttt tgaatcgact
    20761 cttttccact ggtaaatggc ggattaatgc cgaattgcag aaaaacacgt tttttaggtt
    20821 tatcagcata ttgcgctttc aattgcgcgt actgatccag cagggattgc gcggcttgtt
    20881 cggccttatc cggttgcgga ctccaggggg ccaattgacg taacgcattg gcaatttgtt
    20941 caatgcttgt cgcatcgacc cacatcactt ttattcccag cgaagccagc tggtcaacct
    21001 gccgctcggc attacctcca cgccaggcaa tcaccagatc gggtttcagc gcgacaatgc
    21061 gttccagatt catcccctgc caggtggaaa cctgctcaat cttttgcgct tgtggaggat
    21121 agtcggaata gctgctgacc ccaaccggcg tgatcccggc ggcaaaggca agttcagtgt
    21181 tggcgggaga aagcgtgatg acgcgcggcg cggcgttgag ccacagtggc gcaagaaaag
    21241 acagggcgac cagcgccctg aacagtgact tagccatgtg ccagtttctg caccagtgac
    21301 tcaaccatca ggctggactg tttagcggca acagccagga actcatcgaa gctaagatga
    21361 gactgttgat cggccacgtc ggagatggcg cgtacgacaa caaacgggac gttgaaattg
    21421 tggcagacat gggcgattgc cgtcgcttcc atctctacag caatggcctg tgggaagttg
    21481 tggcggattt tcgccagacc aacagaaccg ttgatgaaag cgtcgccgct aacaatcagg
    21541 ccacgtacag cgttaagatt cagttcggca atgcaggcct cagcggcagc gatcagttta
    21601 tcgtcagctt taaagcctgc cggacagcct ggtaactgac cgtattcata accaaatgcc
    21661 gtgacatccg cgtcgtgata acgcgcttcg tccgagacaa cgatatcgcc cactttcagc
    21721 gttggtgcca ggccaccggc agaaccggtg ttaataatca catctggctt gcagtgttcc
    21781 aacagcagag tggcacccag cgccgcagcg actttaccga tgcccgattt cagaagcgca
    21841 acctcggttc cattcagttg gccggtatag atttcgcaac cgccgagact gatagtttga
    21901 cggttttcga ttttgtcacg cagcagcgta acttcttctt ccattgcacc aatgatgccg
    21961 attttcatag atttactcgc gataagcccg atttgaaagc atagtttacc atgcgcttac
    22021 ggggaagcgt atttctcacg cgggagagga catggcacag attgatttcc gaaaaaaaat
    22081 aaactggcat cgtcgttacc gttcaccgca gggcgttaaa accgaacatg agatcctgcg
    22141 gatcttcgag agcgatcgcg ggcgtatcat caactctccg gcaattcgtc gtctgcaaca
    22201 aaagacccag gtttttccac tggagcgcaa tgccgccgtg cgcacgcgtc ttacccactc
    22261 gatggaagtc cagcaggtgg gacgctacat cgccaaagaa attttaagcc gtctgaaaga
    22321 gcttaaattg ctggaagcat acggcctgga tgaactgacc ggcccctttg aaagcattgt
    22381 tgagatgtca tgcctgatgc acgatatcgg caatccgccg tttggtcatt ttggcgaagc
    22441 agcgataaat gactggtttc gccaacgttt gcacccggaa gatgccgaaa gccagcctct
    22501 gactgacgat cgctgcagcg tggcggcact acgtttacgg gacggggaag aacagcttaa
    22561 cgagctgcgg cgcaagattc gtcaggactt atgtcatttt gaggggaatg cacaaggcat
    22621 tcgtctggtg catacattga tgcggatgaa tctcacttgg gcacaggttg gcggtatttt
    22681 aaaatatacc cgtccggcgt ggtggcgtgg cgaaacgcct gagacacatc actatttaat
    22741 gaaaaagccg ggttattatc tttctgaaga agcctatatt gcccggttgc gtaaagaact
    22801 taatttggcg ctttacagtc gttttccatt aacgtggatt atggaagctg ccgacgacat
    22861 ctcctattgt gtggcaga
//
LOCUS       DADMKF010000057        22335 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.058, whole genome shotgun sequence.
ACCESSION   DADMKF010000057 DADMKF010000000
VERSION     DADMKF010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22335
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.058"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(134..3283)
                     /locus_tag="JNC24_002028"
     CDS             complement(134..3283)
                     /locus_tag="JNC24_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HAY9158774.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTSLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSIPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            complement(3306..4499)
                     /gene="acrA"
                     /locus_tag="JNC24_002029"
     CDS             complement(3306..4499)
                     /gene="acrA"
                     /locus_tag="JNC24_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HAY9158775.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYLDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            4641..5288
                     /gene="acrR"
                     /locus_tag="JNC24_002030"
     CDS             4641..5288
                     /gene="acrR"
                     /locus_tag="JNC24_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HAY9158776.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            5416..8778
                     /gene="mscK"
                     /locus_tag="JNC24_002031"
     CDS             5416..8778
                     /gene="mscK"
                     /locus_tag="JNC24_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HAY9158777.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(8990..9151)
                     /gene="rsmS"
                     /locus_tag="JNC24_002032"
     CDS             complement(8990..9151)
                     /gene="rsmS"
                     /locus_tag="JNC24_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HAY9158778.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPARTVLRALDIVKRDYEKK
                     LTRDDKAEK"
     gene            complement(9165..9692)
                     /gene="priC"
                     /locus_tag="JNC24_002033"
     CDS             complement(9165..9692)
                     /gene="priC"
                     /locus_tag="JNC24_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HAY9158779.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVSQFATLSARFDRHLFQTRAT
                     TLQACLDEAGDNLAALCHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAP
                     PKIARWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            9762..10139
                     /locus_tag="JNC24_002034"
     CDS             9762..10139
                     /locus_tag="JNC24_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HAY9158780.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            10292..10843
                     /gene="apt"
                     /locus_tag="JNC24_002035"
     CDS             10292..10843
                     /gene="apt"
                     /locus_tag="JNC24_002035"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HAY9158781.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISETYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            10972..12897
                     /gene="dnaX"
                     /locus_tag="JNC24_002036"
     CDS             10972..12897
                     /gene="dnaX"
                     /locus_tag="JNC24_002036"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HAY9158782.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEANGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVVPTAVMTPTQVPPQ
                     SAPQQAPTVPLPETTSQVLAARQQLQCVQGATKAKKSESAAATRARPVNNAALERLAS
                     VTDRVQARPVPSALEKASAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPELA
                     AKLAAEAIERDPWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNRGA
                     QQKLAEALSMLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNIQT
                     LRRFFDAELDEESIRPI"
     gene            12950..13279
                     /locus_tag="JNC24_002037"
     CDS             12950..13279
                     /locus_tag="JNC24_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HAY9158783.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            13279..13884
                     /gene="recR"
                     /locus_tag="JNC24_002038"
     CDS             13279..13884
                     /gene="recR"
                     /locus_tag="JNC24_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HAY9158784.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            13994..15868
                     /gene="htpG"
                     /locus_tag="JNC24_002039"
     CDS             13994..15868
                     /gene="htpG"
                     /locus_tag="JNC24_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HAY9158785.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWEKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTHTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            16049..16693
                     /gene="adk"
                     /locus_tag="JNC24_002040"
     CDS             16049..16693
                     /gene="adk"
                     /locus_tag="JNC24_002040"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HAY9158786.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYFKEAEAGNTKYAKVDGTKSVAEVRADLEKILG"
     gene            16825..17787
                     /gene="hemH"
                     /locus_tag="JNC24_002041"
     CDS             16825..17787
                     /gene="hemH"
                     /locus_tag="JNC24_002041"
                     /EC_number="4.99.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HAY9158787.1"
                     /translation="MRQTKIGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEGGSPLMVYSRQQQQALAQRLPETPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMAPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(17784..18743)
                     /gene="aes"
                     /locus_tag="JNC24_002042"
     CDS             complement(17784..18743)
                     /gene="aes"
                     /locus_tag="JNC24_002042"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HAY9158788.1"
                     /translation="MKPENKLPVLDLISAEMKTVVNTLQPDLPSWPATGTIAEQRQYY
                     TLERRFWNAGAPEMATRAYMVPTKYGQVETRLFCPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGIDYTLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
                     FAGDSAGAMLALASALWLRDKQIDCGKIAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLA
                     AHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL"
     gene            18895..20199
                     /locus_tag="JNC24_002043"
     CDS             18895..20199
                     /locus_tag="JNC24_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HAY9158789.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEALYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            complement(20332..22008)
                     /locus_tag="JNC24_002044"
     CDS             complement(20332..22008)
                     /locus_tag="JNC24_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kef family K(+) transporter"
                     /protein_id="HAY9158790.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKAIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSVAALFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVTYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(22246..>22335)
                     /locus_tag="JNC24_002045"
     CDS             complement(22246..>22335)
                     /locus_tag="JNC24_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY9158791.1"
                     /translation="SIELVYKICAFLPLLGMLTIFLPDNRHKD"
BASE COUNT         5439 a         5919 c         5856 g         5121 t
ORIGIN      
        1 ttttatgaat tttatccgtg gttaatactg gttttcgtat gagatcctga gttggtggtt
       61 caattactcc ttaatgttcg taggttatgc ataaaaaagg ccgcttgcgc ggccttagtg
      121 attacacgtt gtatcaatga tgatcgacag tatggctgtg ctcgatatct tcattcttgc
      181 ggctaaagcg gcggcgaacc accacaaaga ataccggaac gaagaagatt gccagtaccg
      241 ttgcggtcac catcccgccc attacaccgg tacctactgc gttctgcgcg ccggaaccag
      301 caccagtact gataaccagc ggcataacgc cgaggataaa cgccagcgag gtcatcagga
      361 tcggacgtaa acgcatccgc actgcatcaa gcgtcgcttc aatcagacct ttaccttctt
      421 tatccatcaa gtctttggcg aattcgacga taagtatcgc gttcttcgcc gacaacccaa
      481 tggttgtgag caggcctacc tggaagtaaa cgtcattggt caggccacgg aaggtggcag
      541 ccagcaacgc accgataacc cccagcggaa cgaccagcat aacggagaac ggaatcgacc
      601 agctctcgta cagcgccgcc agacacagga atacgacaat caacgaaatc gcgtacagtg
      661 aaggtgcctg gttgccggag agacgttcct gataggacat ccccgtccag tcatagccaa
      721 caccggtagg cagtttgctc gccagttgtt ccatcagctc cattgcttca ccggtacttt
      781 tacccggtgc cgcctggcct aagatttcca tggatggcag gccgttgtaa cgttccagac
      841 gcggcgaacc gtactcccaa cgagaagagg agaacgccga gaatggcacc atctgaccat
      901 cagcagcacg aacataccag tcgccgatat catccggcag catacggtat ttcgcttctg
      961 acatgacgta aactttcttc acacgaccgc ggtcgataaa gtcgttcaca tagctaccgc
     1021 cccatgcagc gcccagagag gtgttaatgt cgttgataga aacacccagc gcctgcgctt
     1081 tttcctggtc gatatcaatc ttaaactgcg gggtatcttc cagaccgttt ggacgtacgc
     1141 tggtcagcat atcagggtgc ttcgctgctt ctgcaagcag ctggttacgc gcctgagtca
     1201 gtttttcgtg accaaggcca gcctggtcaa tcagctcaaa gtcaaagccg gttgcagtac
     1261 ccagttccac gattgcgggc aggttaaagg cgaaaaccat cgcatctttg atttgcgaga
     1321 aagcgcgtgt tgcacgcatg gtaatcgctt caactttgtt ttcttcgccc ggacgatcgg
     1381 cccagtcctt caaggaaacg aacgcaatac cggtattctg accacgtccc gcaaagccga
     1441 agccgttaac ggcgaacacc gactcaacgt tgttcttttc tttggtcaga tagtaatgcg
     1501 ttacctcatt gagcactttc tgcgtacgtt cctgcgttgc acctgctggc agctgaacca
     1561 tggtcataaa tacgccctgg tcctcatctg gcaagaagga gcttggcaga cgcacgaaca
     1621 gataggccat gccgaccacg atgatcagat acagcaccag gtaacgcccc gtactgcgca
     1681 gaataccgcc tacgctgtcg gtgtagtggt gcgtgctctt ctcgaacatg cggttaaacc
     1741 agccgaagaa gcctttttta ccttccccgt gatcgccttt ggcaatcggt ttcagcatgg
     1801 tggcacaaag agccggagtc aggatcaacg ctaccagtac cgacagcgcc attgctgaaa
     1861 caatggtaat agagaactga cgatagatag caccagtaga accgccaaag aaggccatcg
     1921 gtacgaatac cgccgacagt accatcgcga taccgaccag agcgccctga atctgcccca
     1981 tcgacttacg ggtagcttct tttggcggca aaccttcttc cgccataaca cgctccacgt
     2041 tttctaccac aacgatagcg tcatccacca acaagccgat ggcgagcacc atcccgaaca
     2101 ttgttagcgt gtttatcgag aagccaaagg cggcaaggac ggcaaaggtc ccgagcaata
     2161 ccaccggtac ggcaatggtc ggaatcaacg tcgcgcggaa gttctgcagg aacagataca
     2221 taaccaggaa cacgaggatg atcgcttcga ccagcgtttt aaccacttcg tgaatagaga
     2281 ttttcacgaa cggcgtggtg tcgtacgggt aaacaatttt cagacccgac gggaagaacg
     2341 gttccatctt cgccagttca gcacggattg ccgcagcggt atccagcgcg tttgcaccgg
     2401 tcgccagctt gatccccaga ccggaagccg gttggccgtt aaactctgcg atgatgtcgt
     2461 agttctcacc acccagctca atcttcgcca catcacgcag cagtacgcgg gaaccatcct
     2521 gattcacttt cagcaggatt ttgccgaact cttcagtaga ggtcagacgc gtctgagcaa
     2581 taatagaggc gttaagctgt tggcctttca ccggcggcgt accaccgagc tgacccgccg
     2641 caacctgggc gttctgcgct ttgatggcgg taatgacatc aaccggcgtt agctggaatt
     2701 tgttcagctc attcgggttc atccagatac gcatcgcgta ctgtgaaccg aacaactgaa
     2761 catcacccac gcccgacgta cggctgatgg catctttcat attcgccgct acgtagtcag
     2821 agatatcctc ctgcgtcatg gtgccatcgg tgttgataac gccgacaacc atcaggaagc
     2881 tgctggatga tttctcaacg ctcacccctt gctgctgaac ttcttgcggc agcaacggca
     2941 tcgccagctg cagtttgttc tgcacctgaa cctgcgcgat atccgcatca gtaccagact
     3001 caaaggtcag ggtgatctgc acggtacccg tggagtcact gttagaggac atgtacatca
     3061 ggttatcgat accgttcata ttctgttcga taacctgtgt caccgtgtcc tgcactgttt
     3121 tcgcatcagc gccggggtag gaggcggaga tcgttactgc cggcggtgca atcgtaggat
     3181 attgcgccac cggcagtttg aggatcgcca gcccccctgc caacatgatg ataatggcga
     3241 tcacccacgc aaaaatcggg cgatcgataa agaaattagg catgtcttaa cggctcctgt
     3301 ttaagttaag acttggactg ttcaggctga gcaccgcttg cggcttgctg gttattatca
     3361 gcggtaactt cttgtgcttt tacctggaca ccaggacgca ctttctgcag cccacttatt
     3421 actacgcgat cgcctgcttt cagaccttct gtcaccagcc acttatcgcc aatagcctgg
     3481 cttgcaacga tcggacgggt ttccactttg tcatccgcgc caactaccag tacggtggca
     3541 tcaccacgcg gcgtacgggt tacgccctgt tgcgggacta aaatagcgtt tggattaagc
     3601 ccttcttcca gacgtgcgcg cacgaacata cccggcagca gagtgtgatc cgggttcggg
     3661 aagatagcgc gtagggtgat agacccagtg gtctgatcaa cggtaacgtc agagaattcc
     3721 agcgtaccgt cctgcgggaa cttaatgccg tcactggtga tcagcgacac tttggctttg
     3781 ccgttctctt gtttcagcgt gccattcgcc agttcctgtt tcaggcgcag gaagtcgttg
     3841 ctggactggg tcacatcaag gtagatcgga tcaagttgct gcacggttgc cagcgcagtc
     3901 gcctgaccgt tctgtaccaa tgcgccttcc gtcacgttcg acttaccaat gcgaccgcta
     3961 atcggagagg tgactttggt gtaagccaga ttgatccgcg cagtttcaac ggcagctttc
     4021 gccgcagtta ccgcagcatt cgcctgttgc gcatcagcca gagcctgatc gtactcttgc
     4081 ttactgatgt actgagtacc gagcagtttc tgataacgat tcaccgtcaa ttgcgcgata
     4141 ttggctgcag cctgggcttt cgccagatca cctttcgcac tgtcgtatgt cgcctgatag
     4201 gtcgcaggat caatctgata gagagagaca ccagcttcga tgtcgctacc ttctttgaaa
     4261 ttacgcttca ggataatccc gctaacttga ggacgaactt ctgcgatccg gtaggcactg
     4321 gtgcgacccg gaagctcggt tgtgatctgc agaggttcag ttttgactgt tactacgcca
     4381 acggcgggca tctgctggcc accttgttgg gcctgtttgt cgtcacatcc tgttagggct
     4441 aagctgcctg agagcatcag aacgaccgcc agaggcgtaa accctctgtt tttgttcata
     4501 tgtaaacctc gagtgtccga tttcaaattg gtcaatggtc aaaagttaat aaacccattg
     4561 ctgcgtttat attatcgtcg tgctatggta catacattca caaatgtatg taaatctaac
     4621 gcctgtaaat tcacgaacat atggcacgaa aaaccaaaca agaagcgcaa gaaacgcgcc
     4681 aacacatcct cgatgtggct ctacgtcttt tctcacagca gggggtatca tccacctcgc
     4741 tgggcgagat tgcaaaagca gctggcgtta cgcgcggtgc aatctactgg cattttaaag
     4801 acaagtcgga tttgttcagt gagatctggg aactgtcaga atccaatatt ggtgaactag
     4861 agcttgagta tcaggcaaaa ttccctggcg atccactctc agtattaaga gagatattaa
     4921 ttcatgttct tgaatccacg gtgacagaag aacgacgtcg attattgatg gagattatat
     4981 tccacaaatg cgaatttgtc ggagaaatgg ctgttgtgca acaggcacaa cgtaatctct
     5041 gtctggaaag ttatgaccgt atagaacaaa cgttaaaaca ttgtattgaa gcgaaaatgt
     5101 tgcctgcgga tttaatgacg cgtcgggcag caattattat gcgcggctat atttccggcc
     5161 tgatggaaaa ctggctcttt gccccgcaat cttttgatct taaaaaagaa gcccgcgatt
     5221 acgtcgccat cttactggag atgtatctcc tgtgccccac gcttcgtaat cctgccacta
     5281 acgaataacc ctgaatctga ctccaggatt tttcctggac attttcgtcg ttgctattct
     5341 ggttcactgc gtcgtgatat tcttgcggtt tgactttttc aggtcgttct tcaggttcag
     5401 aaaccttcat tcatcatgac tatgttccag tattacaaac gatcacggca ttttgttttt
     5461 tcagcattta ttgcttttgt ttttgtcttg ttatgccaga acacggcgtt tgcgcgggcg
     5521 tcatcgaatg gtgatctgcc gacaaaagcg gacctgcagg cgcaacttga ctcactaaat
     5581 aaacaaaaag atctttctgc tcaggacaaa ctggtgcagc aggatctgac agatacatta
     5641 gccaccctcg ataaaatcga tcgcataaaa gaagagacag ttcagctacg gcaaaaagtc
     5701 gctgaagcgc cagaaaaaat gcgccaggcg accgcggcgt taacagcact tagcgatgtc
     5761 gataacgacg aagaaacgcg caaaattctg agtacgcttt cgttgcgcca gctggaaact
     5821 cgcgttgccc aggcgctgga cgatttgcaa aacgcacaaa acgatctggc gtcttataac
     5881 agccagctgg tttcgttaca gacgcagccc gaacgcgtgc aaaatgcgat gtataacgct
     5941 tcgcagcagc tgcaacaaat tcgcagtcgt ctggatggga ctgatgtcgg cgagactgcc
     6001 ttacgtccca gccagaaagt gttaatgcag gctcagcagg cgttgctgaa tgcggagatt
     6061 gaccagcagc gtaaaagcct ggaagggaac accgtcttgc aggatacctt gcaaaagcaa
     6121 cgtgattacg tgacggcgaa cagcgcccgt ctggagcacc agttgcagct gttgcaagaa
     6181 gcggtaaaca gcaagcgcct gactttaact gaaaaaacgg cgcaggaagc cgtctccccg
     6241 gatgaagccg cgcgtattca ggctaatccg ctggtgaaac aggaactgga aattaaccag
     6301 cagttaagtc agcgtctgat taccgcgact gaaaacggta atcagttgat gcagcaaaac
     6361 attaaagtca aaaactggct ggagcgggcg ctgcaatcgg aacgcaatat taaagagcag
     6421 attgccgtcc tgaagggcag cctgctgttg tctcgtatcc tttaccagca acaacaaacg
     6481 ctgccctcgg cggatgaact ggaaaacatg accaaccgca tcgcggattt gcgtctcgaa
     6541 cagtttgaag ttaaccagca gcgtgatgca ctcttccaga gcgatgcgtt cgtcaacaaa
     6601 ctggaagaag gtcacaccaa cgaagtcaac agcgaagttc acgatgcgtt attgcaagtg
     6661 gttgatatgc gtcgcgaatt gctggatcaa ctcaacaaac agttgggtaa ccagctgatg
     6721 atggccatta acctgcaaat caaccagcag cagttaatga gcgtatcgaa aaacctgaaa
     6781 tccatcctga ctcagcaaat cttttgggtg aacagtaacc gtccaatgga ctgggactgg
     6841 atcaaagcgt tcccgcaaag cctgaaagat gaatttaagt cgatgaaaat cacggtgaac
     6901 tgggaaaaag cctggcctgc cgtttttatc gctttcctcg ccggtttgcc gctgctgttg
     6961 attgccgggc tgatccactg gcgtctgggc tggctgaaag cgtatcaaca gaaactggct
     7021 tccgctgtgg gttccctgcg taacgatagc cagctcaaca caccaaaagc gatccttatc
     7081 gacctgatcc gcgcgctgcc ggtgtgcctg attattctcg cggttggcct gattctgttg
     7141 accatgcagc tcaacatcag cgaactgcta tggtcgttca gtaaaaaact ggcgattttc
     7201 tggctggtgt ttggcctgtg ctggaaggta ctggagaaaa atggcgttgc cgtgcgtcac
     7261 ttcggcatgc cggagcagca gaccagccac tggcgtcggc aaattgtccg catcagtctc
     7321 gcgttgctgc ctatccattt ctggtctgtg gtggcagaac tttccccgct gcatctgatg
     7381 gatgatgtgc tggggcaggc gatgattttc ttcaacctgc tgctgattgc cttcctggta
     7441 tggccaatgt gccgcgaaag ctggcgtgat aaagagtcgc acaccatgcg actggtcacc
     7501 attaccgtgc tgtcgattat cccgattgca ttgatggtgc tgactgccac gggttatttc
     7561 tacaccacgc tgcgcctctc cgggcgttgg attgaaaccg tttatctggt gatcatctgg
     7621 aacttgctct accagacggt attgcgcggc ttaagcgtag cggcgcggcg tatcgcctgg
     7681 cgtcgtgcgc tggcgcgtcg gcagaatctg gtgaaagagg gcgcagaagg tgccgaaccg
     7741 ccggaagaac ccaccattgc actggaacag gttaaccagc agacgctgcg tattaccatg
     7801 ttgctgatgt ttgcgctgtt cggtgtcatg ttctgggcaa tttggtccga tttgatcacc
     7861 gtgttcagct atctcgacag catcacgctc tggcattaca acggcactga agctggcgct
     7921 gcggtggtga aaaacgtcac catgggcagt ctgttgtttg cgattatcgc ctcaatggtg
     7981 gcctgggcgt tgattcgcaa cctgcctggt ttactggaag tgctggtgct ctcgcgactg
     8041 aatatgcgtc agggcgcgtc gtatgccatt actaccatcc ttaactacat cattattgcc
     8101 gttggtgcga tgacggtgtt cggatcgctg ggcgtctctt gggataaact ccagtggctg
     8161 gccgcagcat tatccgtagg tcttggtttt ggtttacaag aaattttcgg taacttcgtc
     8221 tccggtttga tcattctgtt cgaacgtccg gtgcgtattg gcgatacggt aaccattggt
     8281 agcttctcgg ggacggtaag taagatccgt attcgtgcga caacgattac cgatttcgat
     8341 cgcaaagaag tgatcatccc gaacaaagcg tttgttaccg agcgtctgat caactggtcg
     8401 ttgactgaca ctactacgcg tctggtgatc cgtctcggcg tggcctatgg ctccgatctg
     8461 gaaaaagtgc gtaaagtgtt actgaaggcg gcgactgagc acccaagggt gatgcacgaa
     8521 ccaatgccgg aagtcttctt tacggcgttt ggtgccagca cgttggatca tgagctgcgt
     8581 ctgtatgtgc gtgaactgcg tgaccgtagt cgtactgtcg atgagctgaa ccgtactatc
     8641 gatcagctgt gccgtgaaaa cgacatcaac attgccttta accagcttga agtgcatctg
     8701 cacaacgaga agggcgatga ggtgacggaa gtcaaacgcg actacaaagg cgatgacccg
     8761 acgccagcgg tagggtaata accaaagggg gcgacattta gttgccccga gattactgac
     8821 aaagtgcgcg ttgttcatgc cggatgcggc gtgaccgcct tatccggcct acgaaaccgc
     8881 aagaattcaa tgtattgcag gagcggtgta ggcctgataa gcgtagcgca tcaggcagtt
     8941 ttgcgtttgc ccacaatctt aggggacatt tagcggcccc atttatttct cacttttccg
     9001 ccttatcatc gcgcgttaat ttcttttcat aatcgcgttt tacaatatcc agcgcgcgca
     9061 gcacggtgcg ggctgggatc tgattttctt ccagcagcac aatcaaatcg acagccagtt
     9121 tgacatcgtc aggggcattt tcaagtgaca tactctctcc attgctagcg ggttaagcgc
     9181 gctagcctgt tttcgatttt ttccagcgca tggcggcagc gtgccaggcg cgcttcatag
     9241 gcttccactt cacgatgcag cgtttgctgt tccacgagat cggtcacccg cgccaggcgg
     9301 gctctgcgtt cggcaaccat ctcacgtagc cgccgctcaa aatcctgatg ctgaatacgt
     9361 ttacgctgcc agcgggcaat tttcggtggc gcactgtccc actcgcgcaa tgaccaggcg
     9421 gaggcttcac gcgcgatggc ttccagttgt gccgccagat gttccgccag ccaggccact
     9481 tgcggcagct gttgctgctc aaccgcatga caaagcgcag ccagattatc gcccgcctcg
     9541 tcgagacaag cctgtagtgt tgtcgcacga gtctgaaaaa gatgcctgtc gaaacgagca
     9601 cttagcgtgg cgaactgtga caccggggca caacgctgac gcagcgtagc gagctgtcct
     9661 tccagttttt ccagcagcag ggcggttttc acgataagac ctcaatgaaa atgataattg
     9721 ttatgctaaa gtagccactc tgtaagctga caacactcaa tatgcaacga atcattttaa
     9781 tcatcattgg ctggctggcg gtagtgctgg gtacgctggg cgtggtatta ccggtattac
     9841 cgacgacgcc gtttatcctg ctggcggcct ggtgctttgc ccgttcttcc ccgcgctttc
     9901 acgcctggtt gctgtaccgc tcatggtttg gcagctatct acgtttctgg cagaaacatc
     9961 atgcgatgcc gcgcggcgtc aaaccgcggg cgattttgct tattttgctc acgtttgcca
    10021 tttctctgtg gttcgtccag atgccatggg tgcgcatcat gttgctggta attctcgcct
    10081 gtttgctttt ctatatgtgg cgaattccgg tgattgatga aaagcaagaa aagcactgaa
    10141 gcacaacaat cgcagttgca attattgcgt acagccagta cattctggcg ttttcgagca
    10201 caggcgcagg cggtcaaagg ttaaacaact gttacttttg atacgtttaa aacgcgccgt
    10261 gagtaccacc gtaacaagca ggcatacact tatgaccgcg actgcacagc agcttgagta
    10321 tctcaaaaat agcatcaaaa gcattcagga ctacccaaaa cccggcattc ttttccgcga
    10381 tgtcaccagc ttactggaag acccgaaagc ttacgctctc agcatcgact tgctggttga
    10441 gcgttacaaa aatgcgggca ttaccaaagt tgtcggcacc gaagcgcgtg gcttcttgtt
    10501 tggcgctccg gtagctctgg gtctgggcgt tggctttgta ccggtccgta aaccgggcaa
    10561 actgccgcgt gaaaccatca gtgaaactta cgacctggaa tacggcaccg atcagctgga
    10621 gatccacgtt gatgccatca aaccgggcga caaagttctg gtggtggacg atctgctggc
    10681 aaccggcggc actatcgaag cgaccgttaa actgatccgt cgtctgggtg gtgaagtggc
    10741 tgacgctgcg ttcattatca acctgttcga tctcggcggc gaacagcgtc tcgaaaaaca
    10801 gggcattacc agctacagcc ttgtcccgtt cccgggccat taattatcgc cagtcttgtg
    10861 ctgcccacgc tacggacagc acaagatgtg cattcagcct cgccgttctg acggggctgt
    10921 gttagcatta ccccttcgtg aatccacctt ccagcgtttc agagcctgcc aatgagttat
    10981 caggtcttag cccgaaaatg gcgcccacaa acctttgctg acgtcgtcgg ccaggaacat
    11041 gtgctgaccg cactggcgaa cggcttgtcg ttagggcgta ttcatcatgc ttatcttttt
    11101 tccggcaccc gtggcgtcgg aaaaacctct atcgcccgac tgctggcgaa ggggctaaac
    11161 tgcgaaaccg gcattaccgc gacgccgtgc ggcgtgtgcg ataactgtcg tgaaatcgag
    11221 caggggcgct ttgtcgatct gattgaaatc gacgccgcct cgcgcaccaa agttgaagat
    11281 acccgcgacc tgctggataa cgtccagtac gctccggcgc gtggtcgttt caaagtttat
    11341 ctgatcgacg aagtgcatat gctgtcgcgc cacagcttta acgcactgtt aaaaaccctt
    11401 gaagagccgc cggagcacgt taagtttctg ctggcgacga ccgatccaca gaaattgccg
    11461 gtgacgattt tgtcgcgctg tttgcaattt catctcaagg cgctggatgt cgagcaaatt
    11521 cgccatcaac ttgagcacat cctcaacgaa gaacatatcg ctcacgagcc gcgggcgttg
    11581 caattgctgg cgcgcgccgc tgaaggcagc ctgcgagatg ccttaagtct gaccgaccag
    11641 gcgattgcca gcggtgacgg ccaggtttca acccaggcag tcagtgcgat gctgggtacg
    11701 cttgatgacg atcaggcgct gtcgctggtt gaagcgatgg tcgaggccaa cggcgagcgc
    11761 gtaatggcgc tgattaatga agccgctgcc cgtggtatcg agtgggaagc gttgctggtg
    11821 gaaatgctcg gcctgttgca tcgtattgcg atggtacaac tttcgcctgc tgcacttggc
    11881 aacgacatgg ccgccattga gctgcggatg cgtgaactgg cgcgcaccat accgccgacg
    11941 gatattcagc tttactatca gacgctgttg attggtcgca aagaattacc gtatgcgccg
    12001 gaccgccgca tgggcgttga gatgacgctg ctgcgcgcgc tggcgttcca tccgcgtatg
    12061 ccgctgcctg agccagaagt gccacgccag tcctttgcac ccgtcgtgcc aacggcagta
    12121 atgacgccaa cccaggtgcc gccgcaatca gcgccgcagc aggcaccgac tgtaccgctc
    12181 ccggaaacca ccagccaggt gctggcggcg cgccagcagt tgcagtgcgt gcagggagca
    12241 accaaagcaa aaaagagtga atcggcagcc gctacccgcg cgcggccggt gaataacgct
    12301 gcgctggaaa gactggcttc ggtcaccgat cgcgttcagg cgcgtccggt gccatcggcg
    12361 ctggaaaaag cgtcagccaa aaaagaagcg tatcgctgga aggcgaccac tccggtgatg
    12421 cagcaaaaag aagtggtcgc cacgccgaag gcgctgaaaa aagcgctgga acatgaaaaa
    12481 acgccggaac tggcggcgaa gctagcggca gaagccattg agcgcgaccc gtgggcggca
    12541 caggtgagcc aactttcgct accaaaactg gtcgaacagg tggcgttaaa tgcctggaaa
    12601 gaggagagcg acaacgcagt atgtctgcat ttgcgctcct ctcagcggca tttgaacaac
    12661 cgcggtgcac agcaaaaact ggctgaagcg ttgagcatgt taaaaggttc aacggttgaa
    12721 ctgactatcg ttgaagatga taatcccgcg gtgcgtacgc cgctggagtg gcgtcaggcg
    12781 atatacgaag aaaaacttgc gcaggcgcgc gagtccatta ttgcggataa taatattcag
    12841 accctgcgtc ggtttttcga tgcggagctg gatgaagaaa gtatccgccc catttgatcg
    12901 taagcacagc ttacgttcgt catccttaac gtgattgaga gagaaaccta tgtttggtaa
    12961 aggcggtctg ggtaacctga tgaagcaagc ccagcagatg caagaaaaaa tgcagaaaat
    13021 gcaggaagag atcgcgcagc tggaagtcac cggcgaatct ggcgcaggtc tggtaaaagt
    13081 gaccatcaac ggtgcacaca actgccgtcg cgtagagatc gacccgagcc tgctggaaga
    13141 cgacaaagag atgctggaag acctggtggc tgcagcattc aacgacgcag cacgtcgtat
    13201 tgaagaaacg cagaaagaaa aaatggcctc tgtatcctcc ggaatgcagc tgccgcctgg
    13261 ctttaagatg ccgttctgat gcaaaccagc ccgctgttaa cacagcttat ggaagcactg
    13321 cgctgtctgc cgggcgttgg cccgaagtcg gcgcagcgta tggcgttcac gctgcttcag
    13381 cgcgatcgta gcggcgggat gcgtttggcg caggcgctca cccgggcgat gtcggaaatc
    13441 ggccactgcg ccgattgccg cactttcacc gaacaggaag tctgtaacat ctgttcgaat
    13501 ccgcgtcgtc aggaaaacgg tcaaatctgt gtggtggaga gtccggcgga catctacgcc
    13561 attgagcaga cggggcagtt ttctggtcgt tattttgtgt tgatggggca tctgtcaccg
    13621 ctggacggca tcggtccgga tgatatcggg cttgatcgtc tggaacagcg tctggcagag
    13681 gaaaaaatca ctgaagtgat cctcgccacc aatcccacgg ttgaaggtga agctaccgct
    13741 aactacattg ccgagctttg cgcgcaatat gacgtggaag ccagccgaat cgctcatggc
    13801 gtaccggttg gcggcgaact ggaaatggtc gatggcacca cgctgtcaca ctcccttgcc
    13861 gggcgtcata agattcgttt ttaagcaaac gagagcagga tcacctgctc tcgcttgaaa
    13921 ttattctccc ttgtccccat ctctccctca tcctgttttt aaccttaaaa tggcattatt
    13981 gaggtagacc tacatgaaag gacaagaaac tcgtggtttt cagtcagaag tgaaacagct
    14041 tctgcacctg atgatccatt ctctctattc caataaagaa atcttcctgc gtgagcttat
    14101 ctctaacgcc tccgatgcgg cggacaagct gcgtttccgt gcgctatcta acccggacct
    14161 gtacgaaggt gatggcgaac tgcgcgttcg tgtctctttc gataaagaca agcgcacgct
    14221 gaccatctct gataacggcg tggggatgac ccgcgacgaa gtgattgacc atctggggac
    14281 tatcgctaaa tccggtacca aatcattcct cgaatccctg ggttctgacc aggcgaaaga
    14341 cagccagctg atcggtcagt ttggtgttgg tttctactct gcgtttatcg tggccgacaa
    14401 agtgaccgtg cgtactcgcg cggcaggcga aaaaccagaa aacggcgtct tctgggaatc
    14461 ggctggcgaa ggtgaatata ccgttgccga catcaccaaa gaagatcgtg gtactgaaat
    14521 caccctgcat ctgcgtgaag gcgaagacga gttcctcgat gactggcgcg tgcgttccat
    14581 catcagcaaa tactccgacc atatcgcgct gccggtagag atcgaaaaac gcgaagagaa
    14641 agacggcgaa accgttatct cctgggagaa aatcaacaaa gcgcaggcgc tgtggactcg
    14701 taacaagtcg gaaatcaccg atgaagagta caaagagttc tacaaacaca tcgcccacga
    14761 ctttaatgat ccgctgacct ggagccacaa ccgcgttgaa ggtaagcagg agtacaccag
    14821 cctgctgtac attccgtccc aggctccgtg ggatatgtgg aaccgtgatc ataaacacgg
    14881 cctgaaactg tacgtccagc gtgtgttcat catggacgac gcagaacagt tcatgccgaa
    14941 ctatctgcgc ttcgtgcgtg gtctgattga ctccagcgat ctgccgctga acgtttcccg
    15001 tgaaatcctc caggacagca cggtaacgcg taacctgcgc aatgcgctga ccaagcgtgt
    15061 gctgcaaatg ctggaaaaac tggcgaaaga cgacgcggaa aaataccaga ccttctggca
    15121 acagtttggc ctggtactga aagaaggtcc ggcggaagat ttcgctaacc aggaagcgat
    15181 cgccaaactg ctgcgttttg cttctaccca taccgattct tctgcgcaga ccgtatctct
    15241 ggaagactac gtttcccgca tgaaagaagg gcaggagaaa atctactaca tcaccgcaga
    15301 cagctatgcg gcagcgaaga gcagcccgca cctggaactg ctgcgtaaga aaggcatcga
    15361 agttctgctg ctttccgacc gcatcgatga gtggatgatg aactatctga ctgagttcga
    15421 cggtaaaccg ttccagtcgg tgtctaaagt tgacgagtcg ctggaaaaac tggctgacga
    15481 agttgatgag agcgcgaaag aagcggagaa agcactgact ccgttcatcg accgtgtgaa
    15541 agccctgctc ggcgagcgcg tgaaagatgt ccgtctgact caccgtctga ccgatacgcc
    15601 agcgatcgtt tcgaccgacg cggacgaaat gagcactcag atggcgaaac tgttcgctgc
    15661 ggcgggccag aaagtgccag aagtgaaata catcttcgaa ctgaacccgg atcacgtact
    15721 ggtgaaacgt gcggcagata ctgaagatga agcgaagttc agcgagtggg tagaactgct
    15781 gctggatcag gcgctgctgg cagaacgcgg cacgctggaa gatccgaacc tgtttattcg
    15841 tcgtatgaac cagctgctgg tttcctgatg tcatgccgga tgacctttgt gtcatccggc
    15901 atttttcttt tcatcatctg cactttccgc aaattatctc gccattaacc gtttcagcct
    15961 caggtgcctt tcttgaggca atcgcctgtt ggtggtatcg tttatcgctt tttcaaaaaa
    16021 atcgacacat tttaagggga ttttcgcaat gcgtatcatt ctgcttggcg ctccgggcgc
    16081 ggggaaaggg actcaggctc agttcatcat ggagaaatat ggtattccgc aaatctccac
    16141 tggcgatatg ctgcgtgctg cggtcaaatc tggctccgag ctgggtaaac aagcaaaaga
    16201 cattatggat gctggcaaac tggtcaccga cgaactggtg atcgcgctgg ttaaagagcg
    16261 cattgctcag gaagactgcc gtaatggttt cctgttggac ggcttcccgc gtaccattcc
    16321 gcaggcagac gcgatgaaag aagcgggcat caatgttgat tacgttctgg aattcgacgt
    16381 accggacgaa ctgattgttg atcgtatcgt aggccgccgc gttcatgcgc cgtctggtcg
    16441 tgtttatcac gttaaattca atccgccgaa agtagaaggc aaagacgacg ttaccggtga
    16501 agaactgact acccgtaaag acgatcagga agaaaccgtg cgtaaacgtc tggttgaata
    16561 ccatcagatg actgcaccgc tgatcggcta ctacttcaaa gaagcggaag cgggtaacac
    16621 caaatacgcg aaagttgacg gcaccaagtc ggttgctgaa gttcgcgctg atctggaaaa
    16681 aatcctcggc taatcaagcc gcagcagatc tgctttcggg caggtctgct catccacctc
    16741 aggcaatacc tctcacagca attagttctt cttcctcact tttccgctac aattatcaac
    16801 aagttgaatc gataagaggc ggtaatgcgt cagactaaaa tcggtatcct gctggcaaac
    16861 ctgggtacgc ccgatgcccc cacacctgaa gcggtaaaac gctatctgaa acaattttta
    16921 agcgacagac gcgtggttga tacctcacgg ttgttatggt ggccattgct gcgcggcgtt
    16981 attttgccgc tgcgctcgcc gcgtgtggcg aagctgtacg cctcggtctg gatggaaggt
    17041 ggctcgccgc tgatggttta cagccgccag caacagcagg cgctggcaca acgtttaccg
    17101 gagacgcccg tagcgctggg aatgagctac ggctcgccat cactggaaag cgccgtcgat
    17161 gaactcctgg cagagcatgt agatcatatt gtggtgctgc cgctttatcc gcaattctcc
    17221 tgttctacgg tcggtgcggt atgggatgaa ctggcacgca ttctggcgcg caaacgtagc
    17281 attccgggga tatcgtttat tcgtgattac gccgataacc acgattacat taatgcactg
    17341 gcgaacagcg tacgcgcttc ttttgccaaa catggcgaac cggatctgct gctgctctct
    17401 tatcatggca ttccccagcg ttatgcagat gaaggcgatg attacccgca acgttgccgc
    17461 acaacgactc gcgaactggc ttccgcactg gggatggcac cggaaaaagt gatgatgacc
    17521 tttcagtcgc gctttggccg ggaaccctgg ctgatgcctt ataccgacga aacgctgaaa
    17581 atgctcggag aaaaaggcgt aggtcatata caggtgatgt gcccgggctt tgctgcggat
    17641 tgtctggaga cgctggaaga gattgccgag caaaaccgtg aggtcttcct cggtgcgggc
    17701 gggaaaaaat atgaatatat tccagcgctt aatgccacgc cggaacatat cgaaatgatg
    17761 gctaatcttg ttgccgcgta tcgctaaagc tgagcggtaa agaactgagc gccgtcgcga
    17821 agagcctcgt cggcggtttt catcatccgt gaataatgca aaaaggcatg cagcgtacct
    17881 gggtagagtt tgaactcaca gggctgctga tgcgccgcta acgtctggta aagcagacgg
    17941 ctgtcatcca gcagcggatc gaactccgcc ccggcaataa aacagggcgg aacttcgcga
    18001 gtgagatcat tattaaacag acagtaatac ggtgactcgc ggtccgcgtc gttgcttaaa
    18061 tacgcctctt catacatctg caaatcctgt tgcgttaagc catcccagac accgcccaac
    18121 agacgacgag tcacggaatc ccgtaatccg taaagcccat accacagcaa aacacccgca
    18181 attttaccgc aatcgatctg tttatcacgc aaccacaacg cactggcgag cgccagcatg
    18241 gcacctgcgg aatcaccggc aaagccaatg cgggacatat tgatttgata atcctccgcc
    18301 tgctggtgga aataacaaca agcagccaca atttcctcta tcgcttgcgg aaaacgcgct
    18361 tcaggtgaaa gggtgtaatc aataccaatc accgtacatt ggctgtagct tgccagcagg
    18421 cgcatgatgc gatcgtgggt atcgagattg ccgagaataa aaccgcctcc atgcaaataa
    18481 aatagcgtcg ccgggctatc tggctgcgga caaaagagac gtgtttccac ctgcccatat
    18541 tttgttggaa ccatgtaagc tctggttgcc atttctggag cgcccgcatt ccagaatcgg
    18601 cgctcaagcg tgtaatactg tcgttgctca gcaatcgttc ccgttgcggg ccaggacggt
    18661 aaatccggct gaagagtatt cacaacggtc ttcatttcag cagaaataag gtccagaaca
    18721 ggtagtttgt tttccggctt catacaaaaa ctcctttcaa attacgtcat tgtaaggaac
    18781 cactgccatg aaaatgcgat cccgcctgct gatattgaaa ctggctgcgt ctcgcgcgct
    18841 cccgtcagat tgtgttaaca ttcgccgctc agttaaccac ccgtaaaaac aaccatgaaa
    18901 tttcccggta aacgtaaatc caaacattac ttccctgtaa atgcacgcga tccgctgctt
    18961 cagcaattcc agccagaaaa cgaaaccagc gccgcctggg tcgtaggtat tgaccagacg
    19021 ctggtcgata ttgaagcgaa agtggatgat gaattcattg agcgttatgg attaagcgcc
    19081 gggcattcac tggtgattga ggatgacgta gccgaagcgc tttatcagga gctaaaacag
    19141 aaaaacctga ttacccatca gtttgcgggt ggcaccattg gtaacaccat gcacaactac
    19201 tcggtgctcg cggacgaccg ttcggtgctg ctgggcgtca tgtgcagcaa tattgaaatt
    19261 ggcagttatg cctatcgtta cctgtgtaac acttccagcc gtaccgatct taactatcta
    19321 caaggcgtgg atggcccgat tggtcgttgc tttacgctga ttggcgagtc cggggaacgt
    19381 acctttgcta tcagtccagg ccacatgaac cagctgcggg ctgaaagcat tccggaagat
    19441 gtgattgccg gagcctcggc actggttctc acctcttatc tggtgcgttg caaaccgggt
    19501 gaacccatgc cggaagcaac catgaaagcc attgagtacg cgaagaaata taacgtaccg
    19561 gtggtgctga cgctgggcac caagtttgtc attgccgaga atccgcagtg gtggcagcaa
    19621 ttcctcaaag atcacgtctc tatccttgcg atgaacgaag atgaagccga agcgttgacc
    19681 ggagaaagcg atccgttgtt ggcatctgac aaggcgctgg actgggtaga tctggtgctg
    19741 tgcaccgccg ggccaatcgg cttgtatatg gcgggcttta ccgaagacga agcgaaacgt
    19801 aaaacccagc atccgctgct gccgggcgct atagcggaat tcaaccagta tgagtttagc
    19861 cgcgccatgc gccacaagga ttgccagaat ccgctgcgtg tctattcgca cattgcgccg
    19921 tacatgggcg ggccggaaaa aatcatgaac actaatggag cgggggatgg cgcattggca
    19981 gcgttgctgc atgacattac cgccaacagc taccatcgta gcaacgtacc aaactccagc
    20041 aaacataaat tcacctggtt aacttattca tcgttagcgc aggtgtgtaa atatgctaac
    20101 cgtgtgagct atcaggtact gaaccagcat tcacctcgtt taacgcgcgg cttgccggag
    20161 cgtgaagaca gcctggaaga gtcttactgg gatcgttaag ttatcgtcgg ttcgtaggcc
    20221 agataaggcg ttcacgccgc atctggcatt tggctctcga tgcctgatgc gacgctggcg
    20281 cgtcttatca tgcctacata tttttcatat tttacatccg gcaaccaccg tttaccccgt
    20341 caccacttca cccgccggtg gcgtttccag cagttccagc atagtacggg caatttcacg
    20401 ttcacccatc actacctgat tcgcgccacg ttcggtgata tacgtcactt catcgtcata
    20461 atgggcgcgg gcgataatct caatatccgg gtttttcgcg cgggcagacg ccacaatctc
    20521 acccgcttca taaccgttgg ggatcgtcag gatcagccat tttgcacatt ccagatgcgc
    20581 cagttgcata atttcttcgt tcgctgcatt gcccaataca gcgcggaccc cacgctctcg
    20641 cagctcatca acacgggttc gtgacgtctc aatcaccacc agcggaatat cagaggcgag
    20701 caatttctcc cccagcaggc tgcctacacg accgtaaccc accaatagcg catggttgca
    20761 aatatccact gggatctgtt tctcttcttc gattgcctct tccagcgtct gctcttccag
    20821 cgtttcggtc ttcgccagat atttctccag tagtgcgaac agtaccgggt tgagcataat
    20881 cgacaggatc gcccctgcca gtaccaggtt ttgtccggcc tgcggcagta aattcaatgc
    20941 cattcccagt cccgccagga taaacgcgaa ctcaccaatc tgcgccaggc tggcggcgat
    21001 ggttaatgcc gtacgttggg agtgaccaaa cagacggaca aggaacaggg ccgcgaccga
    21061 tttaccaaac agaataattg ccagcgtcgc cagcactgcc agcggttgct gaatcagaat
    21121 taacggatca aacaacatcc cgacggagac aaaaaacagc accgcaaacg cgtcgcgcaa
    21181 tggcagcgta tcgtgggcgg cacggtgact cagttcagac tcgttcagta ccatcccggc
    21241 aaagaacgcg ccaagtgcga aggagacgtc aaacaactcc accgcaccaa aggcaatccc
    21301 taacgccagc gccagcaccg acagggtaaa tagctcgcga gaaccggttg ccgcgctgcg
    21361 tgccataatc cacggcacca gacggcgacc taccagcatc ataatggcga taaatgcgat
    21421 cactttgccg atggtgatcc ccatatcgac tgcaagagtg gcaaagccca catcgccctg
    21481 ttccatcatt cctgccactg cgggcagcaa caccagcgtc agaaccatta ccaggtcttc
    21541 cacaatcaac caaccgatgg cgatttgccc acgctgactg tcaattaatt gccgttcttc
    21601 aagtgcgcgc agtaacacca cggtactggc ggtggaaaga cataaaccga acacgatacc
    21661 ggtcattaac gaccagccca gcacggcaga gagcgccata cccagcagcg tcgccacggc
    21721 tatctgggcg atcgcaccgg gaatggcgat ggcctttacc gccatcaaat ccttcagcga
    21781 aaagtgcaaa ccgacgccaa acatcaacag aatgacgccc agttcagcca gttccggcgc
    21841 aagcttggta tcggcaacaa agcccggagt gaatggtcct gccagcacac ccgctaacag
    21901 atatcccacc agaggagaaa tacgtagttt attggccagc atgccgagga taaaggcgag
    21961 cacaaggccg ccaacaatgg tggtgataag cggggtggcg tgatgcattc cgtctccttt
    22021 tcctggtggt tattgtccat ttttggccgg gaaaaccaaa attacaggta atagtttatg
    22081 acaatttcat tgatgatgtt catgaataat tattgaattt tgcagaaaaa tggaattagc
    22141 tgcaaaaaaa gcacggatcg gaaaacagaa ggcggtttaa cagaggaaag gcttatggcg
    22201 actgtaaaac gatgcagcca aagtttgcct ttggctgcaa tgaaatcagt ctttatgccg
    22261 gttatcaggc aggaatatgg tcaacatccc caatagtggc aggaaagcac agattttata
    22321 gactaactcg atgct
//
LOCUS       DADMKF010000058        22139 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.059, whole genome shotgun sequence.
ACCESSION   DADMKF010000058 DADMKF010000000
VERSION     DADMKF010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22139
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.059"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(<1..524)
                     /locus_tag="JNC24_002046"
     CDS             complement(<1..524)
                     /locus_tag="JNC24_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HAY9158792.1"
                     /translation="MVAKINLLSRLTPLLFVFAPFLAQSNMTVYPMAVSINSQGEGNV
                     RVISKSNEVQYIKATVFRIDNPSTPQENEVEIKSGDANHLVVMPPKFALPAGSSKTVR
                     FVAMEPEQKEKNYRVKFEAVPSIDDVATDKKDLSMQLTVNLIWGIVVSVPPQQPIAKL
                     EVNAAQKLVNAGNQR"
     gene            820..1596
                     /gene="exuR"
                     /locus_tag="JNC24_002047"
     CDS             820..1596
                     /gene="exuR"
                     /locus_tag="JNC24_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HAY9158793.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            1941..2603
                     /locus_tag="JNC24_002048"
     CDS             1941..2603
                     /locus_tag="JNC24_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HAY9158794.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPIFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            2607..2990
                     /gene="mzrA"
                     /locus_tag="JNC24_002049"
     CDS             2607..2990
                     /gene="mzrA"
                     /locus_tag="JNC24_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HAY9158795.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            3137..3505
                     /locus_tag="JNC24_002050"
     CDS             3137..3505
                     /locus_tag="JNC24_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 domain-containing protein"
                     /protein_id="HAY9158796.1"
                     /translation="MKYRIALAVSLFALSAGSYATTLCQEKEQNILKEISYAEKHQNQ
                     NRIDGLNKALSEVRANCSDSQLRADHHKKIAKQKDEVAERQQDLAEAKQKGDADKIAK
                     RERKLAEAQEELKKLEARDY"
     gene            3543..3848
                     /locus_tag="JNC24_002051"
     CDS             3543..3848
                     /locus_tag="JNC24_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HAY9158797.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            3851..4255
                     /locus_tag="JNC24_002052"
     CDS             3851..4255
                     /locus_tag="JNC24_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9158798.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            4245..4544
                     /locus_tag="JNC24_002053"
     CDS             4245..4544
                     /locus_tag="JNC24_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9158799.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            4640..5122
                     /locus_tag="JNC24_002054"
     CDS             4640..5122
                     /locus_tag="JNC24_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HAY9158800.1"
                     /translation="MILSIDSNDANTAPLHKKTISSLSGAVESMMKKLEDVGVLVARI
                     LMPILFITAGWGKITAYSGTQQYMEAMGVPGFMLPLVILLEFGGGLAILFGFLTRTTA
                     LFTAGFTLLTAFLFHSNFAEGVNSLMFMKNLTISGGFLLLAITGPGAYSIDRLLNKKW
                     "
     gene            5192..6205
                     /locus_tag="JNC24_002055"
     CDS             5192..6205
                     /locus_tag="JNC24_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005140183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HAY9158801.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYISLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESTEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPQDEMFASVKKRHLT
                     KCL"
     gene            6470..6835
                     /locus_tag="JNC24_002056"
     CDS             6470..6835
                     /locus_tag="JNC24_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HAY9158802.1"
                     /translation="MDWYLKVIKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEP"
     gene            7077..7433
                     /locus_tag="JNC24_002057"
     CDS             7077..7433
                     /locus_tag="JNC24_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HAY9158803.1"
                     /translation="MQWYLSVLKNYVGFSGRARRKEYWMFTLINAIVGAIINVIQLIL
                     GLELPYLSMLYLLATFLPVLALAIRRLHDTDRSGAWALLFFVPFIGWLVLLVFFCTEG
                     TSGSNRYGNDPKFGSN"
     gene            complement(7484..8380)
                     /locus_tag="JNC24_002058"
                     /pseudo
     CDS             complement(7484..8380)
                     /locus_tag="JNC24_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            8485..9186
                     /locus_tag="JNC24_002059"
     CDS             8485..9186
                     /locus_tag="JNC24_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HAY9158804.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDACPQRENPLIQKLALNMGKQQLIASPEGTMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            9209..9373
                     /locus_tag="JNC24_002060"
     CDS             9209..9373
                     /locus_tag="JNC24_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9158805.1"
                     /translation="MSKKSAKKRQPVKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(9507..10817)
                     /locus_tag="JNC24_002061"
     CDS             complement(9507..10817)
                     /locus_tag="JNC24_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HAY9158806.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AIADAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVRIETHNG
                     VVFTQQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITATMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(10845..12176)
                     /locus_tag="JNC24_002062"
     CDS             complement(10845..12176)
                     /locus_tag="JNC24_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026203.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HAY9158807.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTGMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSPFLAFS"
     gene            complement(12451..13815)
                     /gene="tdcG"
                     /locus_tag="JNC24_002063"
     CDS             complement(12451..13815)
                     /gene="tdcG"
                     /locus_tag="JNC24_002063"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HAY9158808.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAAWMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(13887..14276)
                     /locus_tag="JNC24_002064"
     CDS             complement(13887..14276)
                     /locus_tag="JNC24_002064"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HAY9158809.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQACLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(14290..16584)
                     /gene="tdcE"
                     /locus_tag="JNC24_002065"
     CDS             complement(14290..16584)
                     /gene="tdcE"
                     /locus_tag="JNC24_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HAY9158810.1"
                     /translation="MKIDIDTSDKLYADAWLGFKGTDWKNEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(16618..17826)
                     /gene="tdcD"
                     /locus_tag="JNC24_002066"
     CDS             complement(16618..17826)
                     /gene="tdcD"
                     /locus_tag="JNC24_002066"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HAY9158811.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVVIDTEMNNRSNSFGERIVSSENARVICAVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(17852..19183)
                     /gene="tdcC"
                     /locus_tag="JNC24_002067"
     CDS             complement(17852..19183)
                     /gene="tdcC"
                     /locus_tag="JNC24_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HAY9158812.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLVLKFGYKGDKTKVSLGKLNTISMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(19205..20194)
                     /gene="tdcB"
                     /locus_tag="JNC24_002068"
     CDS             complement(19205..20194)
                     /gene="tdcB"
                     /locus_tag="JNC24_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HAY9158813.1"
                     /translation="MHITYDLPIAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(20293..21231)
                     /gene="tdcA"
                     /locus_tag="JNC24_002069"
     CDS             complement(20293..21231)
                     /gene="tdcA"
                     /locus_tag="JNC24_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HAY9158814.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
     gene            21420..21764
                     /gene="tdcR"
                     /locus_tag="JNC24_002070"
     CDS             21420..21764
                     /gene="tdcR"
                     /locus_tag="JNC24_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HAY9158815.1"
                     /translation="MTGITIFYGDNIIRYVVNTKKGLRPYFKQLPDNYQAKFELNLMS
                     KFSNFIINKPFSAINTAARHIFSRYLLENKHLFYQYFKISNTGIDHLEQLINVNFFSS
                     DRTSFCECNRFP"
BASE COUNT         5805 a         5650 c         5169 g         5515 t
ORIGIN      
        1 cgttgattcc ctgcattaac taatttttgg gcagcattta cttctaactt agcaataggt
       61 tgctgaggtg gaacactaac aacaatcccc caaattaagt taactgttaa ctgcatagag
      121 agatcttttt tatctgtggc aacgtcatca atactgggaa ccgcttcaaa tttaacgcga
      181 taattttttt ctttttgctc tggttccatt gcaacaaagc gcacagtttt gctactaccg
      241 gctggtaaag cgaatttagg tggcataaca accagatgat ttgcatcacc ggatttaatc
      301 tcaacttcat tttcctgagg cgtcgatgga ttatcgatac ggaatactgt cgccttgatg
      361 tactgaactt cattactttt agatattacg cgaacgttgc cttcaccctg actattaatg
      421 gaaacagcca taggataaac agtcatattt gactgtgcca aaaaaggagc gaacacgaat
      481 aaaagcggcg taagccgtga taagagatta attttcgcca ccatataaat ttgatcttac
      541 aatattgttt ccaaaaagtt ggtgtattag agatgtagat ttattgtaag tcaatataaa
      601 aaagcatttt tagttaatat taaatttaaa ggctgggaat taacttactg aaatttagta
      661 gctttcacaa tgacgatact tattttttag gaatttataa atattcacaa tattaattca
      721 atacattaat tcaatcacca aatttatata tgaccaatgg catacccatg aataaagtgg
      781 tatgataact tcgtccgcat tccactccgg gagacgcata tggaaatcac tgaaccacgc
      841 cgtttgtatc aacaacttgc cgctgacctg aaagagcgca ttgaacaggg cgtctatctg
      901 gtgggtgata aactgcctgc cgaacgcttt attgccgatg aaaagaacgt cagccgaacg
      961 gtagttcgtg aagccatcat catgctggaa gttgaaggct atgtggaggt gcgtaaaggt
     1021 tcaggtattc atgtggtttc caaccagccg cgccatcagc aggcggctga caataatatg
     1081 gaattcgcca attacggtcc gtttgagttg cttcaggctc gccagctcat cgaaagtaat
     1141 attgccgagt tcgcggcaac tcaggtaacg aaacaagaca tcatgaaact gatggccatt
     1201 caggaacagg cgcgcggcga acaatgcttc cgtgattccg agtgggattt acagttccac
     1261 attcaggtag ccctggcgac gcagaactcc gccctggcgg ccatcgttga aaaaatgtgg
     1321 acccagcgta gccataaccc gtactggaaa aaactacacg aacacattga ttcccgtacc
     1381 gttgataact ggtgtgatga ccacgatcaa attctcaagg cgttaattcg caaagatccc
     1441 catgctgcta agctggcaat gtggcagcat ctggaaaaca ccaaaatcat gttatttaac
     1501 gaaaccagcg acgatttcga gttcaatgcc gaccgctatc tattcgccga aaacccggta
     1561 gtgcatctcg atacggccac cagtggcagt aaatgaaact cctgtccgac aggcgctgcg
     1621 acgcgcctgt tcgcttcgtc ggtaagcgaa aagtataaag tgtcagcctg tgtaaatcct
     1681 ctcgccaccc tcccctgcat tcagcaaaat cagactccac ggacatgtaa ttttgataac
     1741 gaacaacgtt gacctttgtt acaattagat tcaatttgaa tttatgtttt tgaatgcttt
     1801 cttatctcac gatttaacag ggaatagttc aggctgtgtt gatgtatcaa acccgcagaa
     1861 cataccaaaa cagcaataac attgcggtag tgcatctttt aaaaccagcg tggcgttaac
     1921 cgattcacca ggaataatga atggaacttt tgacccaatt gctgcaagcc ctgtgggcgc
     1981 aggattttga aaccctggcc aatccatcga tgattggcat gttgtatttt gtcttgtttg
     2041 taattttgtt ccttgaaaac ggcttgcttc cagcggcctt tttaccgggc gacagtttac
     2101 tggtattggt cggcgtgttg attgcgaaag gcgcgatggg ttacccgcaa acgattctgc
     2161 tgctgaccgt tgccgccagc ctcggctgct gggtcagcta tattcagggg cgatggctgg
     2221 gcaatacccg caccgtacaa aactggctat ctcatttacc cgcgcattat catcaacgcg
     2281 cacaccatct ttttcataaa cacggtttat cggcgctgtt aattggtcgc tttattgcgt
     2341 ttgtcagaac actgctgccg acgattgccg ggttatcagg gctgaataac gcgcgctttc
     2401 agtttttcaa ctggatgagc ggtctgctgt gggtattgat cctgacaact ctgggttaca
     2461 tgctcggcaa aacgccgata tttttaaagt acgaggacca gctgatgtca tgcctgatgc
     2521 tgctcccggt ggtgctgctg gtgtttggcc tggcaggttc tctggtcgtg ttatggaaaa
     2581 agaaatatgg aaatcggggg taagggatgc aaatacctcg catgtcgctt cgccagctag
     2641 cctggtccgg cactgtttta cttctggtcg gcacgctgtt gctggcctgg tccgcggttc
     2701 gccagcaaga gtctacgctg gcgattcgtg ccgttcatca aggcacaacg atgccagacg
     2761 gtttttcgat ctggcatcac cttgacgctc atggcattcc tttcaaaagt atcaccccca
     2821 aaaacgacac tctgttaatt acatttgact ccagcgacca gagcgccgcc gcaaaagcgg
     2881 tcctcgacag aacattgccc catggctaca tcattgcgca gcaggacaat aacagtcagg
     2941 ctatgcagtg gctgacccgg ttacgggata actctcatcg cttcggataa cttcctgtta
     3001 tccgaaacat atcactcact ttggtgattt caccgtaact gtctatgatt aatgaggcgg
     3061 tggttaacac taccgcttcg ttgtctggac aatcgttcct ttgtaatagg tccgaatcac
     3121 aatggaaggt tcaagaatga aataccgcat cgctttagct gtttctctct ttgctcttag
     3181 tgccggtagt tatgccacta ccctgtgtca ggaaaaggag caaaatatcc ttaaggagat
     3241 cagctatgcc gaaaaacacc aaaaccagaa tcgtattgac ggtctgaata aagccctgag
     3301 tgaagtccgg gccaactgtt cagatagcca gctgcgtgcc gatcatcaca agaaaatcgc
     3361 aaagcagaaa gatgaggtgg cggaacgcca gcaagattta gccgaggcga agcaaaaagg
     3421 cgatgccgat aagattgcca aacgcgaacg gaaactggca gaagcgcagg aagagctgaa
     3481 aaagctggaa gcgcgcgact actaactcac aatagtcact acttactcac ctggagaaaa
     3541 ctatgtcgaa agaacacact acggaacatc tgcgtgctga gttgaaatcc ctttccgata
     3601 cgctggaaga ggtgcttagc tcatctggcg agaagtcgaa agaagagttg agtaagattc
     3661 gtagcaaagc ggagcaggca ctgaaacaga gccgttatcg cctgggtgaa accggtgatg
     3721 ccatcgccaa acaaacccgt gtcgcggcgg cgcgtgctga tgagtatgtg cgtgaaaatc
     3781 cgtggaccgg cgtgggaatt ggcgctgcaa tcggtgtagt gctcggcgtt ctgctgtcgc
     3841 gtcgttaatt atggcggaca ctcatcacgc gcaagggccc ggtaaaagcg ttctgggcat
     3901 cgggcagcga attgtttcca tcatggttga aatggtggaa acacgtctgc ggctggcggt
     3961 ggtggagctg gaagaggaaa aagctaatct ctttcaactt ttactgatgc tgggcctgac
     4021 gatgcttttc gctgcatttg gtcttatgag cctgatggtg ctaattattt gggcggttga
     4081 tccgcaatat cgcctgaatg cgatgattgc caccaccgtg gtgttgctgc tactggcact
     4141 gattggcggt atctggacgt tacgtaaatc gcgtaaatcc accttgctgc gccatacacg
     4201 ccatgagctg gcaaacgatc ggcaactgct cgaggaggag tcccgtgagc agtaaagtcg
     4261 aacgtgaacg acgtaaggcg caactgctta gccagatcca gcaacaacgg ctggatcttt
     4321 ccgccagtcg tcgtgaatgg ctggaggcaa caggcgctta cgatcgtcgc tggaatatgc
     4381 tgctaagtct gcgctcctgg gcgctggttg gcagtagcgt gatggcgatc tggacgattc
     4441 gccatcctaa tatgctggtc cgctgggcca gacgcggttt tggcgtatgg agcgcctggc
     4501 gtctggttaa aacgaccctc aagcagcaac agcttcgcgg ttaacttccc tctggccgga
     4561 gcaattccgg ccttatccct caaatttttt gaagattttt gacagttttc cttgctaaca
     4621 atcatcattc accacgttta tgattctctc catcgacagc aacgacgcta ataccgcgcc
     4681 cttgcacaaa aaaacaatca gcagcctgag tggcgcagtg gagagtatga tgaaaaaatt
     4741 agaagatgtt ggtgtactgg tagcgcgcat tttaatgccg attctgttta ttaccgcagg
     4801 ctggggaaaa atcaccgctt actctggtac ccaacaatat atggaagcaa tgggtgtccc
     4861 gggttttatg ctgccgctgg tgattctgct tgagttcggt ggtggtctgg caatcctgtt
     4921 cggtttcctg actcgcacca cagccctgtt tactgcgggc tttacgctgc tgacggcatt
     4981 tttatttcac agcaactttg ctgaaggcgt caactcgctg atgttcatga aaaacctgac
     5041 aatttctggc ggattcctgc tgctggcaat taccggtccg ggcgcgtata gcatcgaccg
     5101 cctgctgaat aaaaagtggt aagcacgcgc tatacttaac gataaaaaac gaggaggaag
     5161 ctcctcgttt ttgctattgg aggagagaaa aatgggtcaa ctgattgacg gcgtctggca
     5221 tgacacctgg tacgatacca aatctaccgg cggtaaattt caacgttcag cttccgcatt
     5281 tcgtaactgg ctcactgccg atggcgcacc tggccccact ggcacaggcg gttttatcgc
     5341 agagaaagat cgttatcatc tctatatttc actcgcctgc ccgtgggcgc accgcacgct
     5401 gatcatgcgc aaactcaaag gactggaacc gtttatttcc gtttccgtag tgaacccgct
     5461 gatgctggaa aacggctgga cctttgatga cagttttccg ggagcaaccg gagatacgct
     5521 ctatcaacat gaatttctgt atcagcttta tctccacgcc gatccacact acagcggacg
     5581 agttactgtt cccgtactgt gggacaaaaa gaaccacacc atcgtcagca acgaatcaac
     5641 agaaatcatc cgcatgttca ataccgcgtt tgatgcgctg ggcgcgaaag cgggtgatta
     5701 ctacccacca gccctgcaaa cgaaaattga cgaacttaac ggctggattt atgacaccgt
     5761 taacaacggc gtgtataaag ctggttttgc caccagccag caagcctacg acgaggcggt
     5821 ggcgaaagtg tttgaatcgc tggcgcgact ggagcagatt ttaggccagc atcgttacct
     5881 gaccggcaac cagctaaccg aagccgatat tcgcctgtgg actacgctgg tgcgttttga
     5941 tccagtgtat gtgacccact tcaagtgtga taagcaccgc atcagcgatt acctgaatct
     6001 gtatggcttc ctgcgcgata tctaccagat gccggggatc gccgaaactg tcaatttcga
     6061 tcacatccgt aatcattact tccgcagcca taagaccatc aaccctacgg ggattatttc
     6121 aattggtccg tggcaggatc tcgatgaacc gcaggacgag atgttcgctt cggttaaaaa
     6181 aaggcacctt acgaagtgcc tctgataatt agctaagtgc atgttgttca tatcggatgc
     6241 ggcgtgaacg ccttatcaaa cctacaaaac atttatattc aatatgttgc aatgaccgcg
     6301 caggccagat aagcgtagcg catctggcga ttttgcgctt gtcatcagtc ttagcgcgcc
     6361 ttcacagcac tttattaatt ccttgaacga atatttactg ccatttcatt cacgtctatt
     6421 cttaatttgc tgctttaaaa acaagtgatt gagcaacatt gaggcaaaaa tggactggta
     6481 tctgaaagta ataaaaaatt atgtcggttt ccggggtcgt gcgcggcgca aagagtactg
     6541 gatgtttatt ctggtcaaca tcatctttac gttcgtgctg gggctgctgg ataaaatgtt
     6601 aggctggcaa cgcgctggtg gcgaaggcat cctgacgaca atctacggta ttctggtgtt
     6661 tttaccgtgg tgggcagttc agttccgccg cctgcacgac accgaccgct cggcgtggtg
     6721 ggcactgcta ttcttaatcc cgtttatcgg ctggcttatt atcatcgtct ttaactgcca
     6781 ggcgggtacg ccaggcgaaa accgctttgg acctgatcca aaactggaac cttaaaacaa
     6841 atctgctcgc cggatgtctg actcatccgg cttttatcat catgttgatg aatgaataat
     6901 ttactaatgt ttatttaaaa tatttcaatg tcgttatata aattacctat aaaaaataac
     6961 catgtattta tcttaaatat caaaaccttt ataacatccg aaaaagaaag atacgaatag
     7021 catttacaca atacccggaa tagactttcg tcacttttaa ttaaagggat gtttttatgc
     7081 agtggtattt atccgtatta aaaaattatg ttggtttctc tggtcgcgca cgtcgtaaag
     7141 agtactggat gtttactctg attaacgcca tcgtcggcgc tattatcaat gtcattcaat
     7201 tgattttagg tctggagctt ccatatctgt ctatgcttta tttgctggca accttccttc
     7261 ccgtccttgc gcttgctatc cgtcgcttac acgataccga ccgttcaggt gcctgggcct
     7321 tgctcttttt tgttccgttt attggctggc tcgtgcttct ggtttttttc tgcacagaag
     7381 gtacttctgg cagcaatcgt tacggaaacg atccgaagtt tggttcaaat taatcttaga
     7441 attggggcga tatttcgccc ctttttatta acaataataa atattatttt ccgctaaaaa
     7501 gtttgggaat ttcccgcaga caccaggatt tcgcttcccc catactgtca cgacgccagg
     7561 ccataataat atcgatctca ctggtcgatt ccgggctgac gacacgcaac cgcccttccg
     7621 caatatcttt ttcgaccatc ggatacggca tcgtcgccac gccaagcccc gccagtaatg
     7681 cctgacgttt atcttcaatc gtgctcaccg ttaagcgcgg ctgtttgtcc agcagttgta
     7741 cggtcaacac cgggcgctca cgagcggtat ccgctaccgc aattccacga tatttcacgc
     7801 gcgtcacttc agataacggt tccggctcct gatgaatcgg gtgatcaggc gcggcaacgt
     7861 agacgttcat taacgtatag agcttgcgcg agttgatctc cgacgaggaa cgaaaatgca
     7921 tatccggcgc gataacaata tccgcccgcc cctgctccag tcgttcctac gcccccgcca
     7981 gcacttcggt gatgattgcc agttgggtat tggcttttgc cgccagtttg tcgattaacg
     8041 ggaaaaaggc aggtgtcggt accagcgctt cggtcacaat ggtgagatgc gtttcccaac
     8101 cgcgcgcgag ggcttccgca tcggtagtca gtttatctgc ggcttccagc aaaacgcgcc
     8161 ctcgctccag caacatcctc ccgacattgg tgaatttggt gcgatggccc gagcggtcaa
     8221 acagcaccac atccagctct tcttccagtt tttgcatggt gtagctaagt gcggaaggca
     8281 cgcgtcccag ctcatccgcc gccgccgcaa aactgccccg gcgatcgatc gcatccataa
     8341 cccgtagtgc ttccagcgtt aatgcccttt ctttggccat ttcgttctca ttcaggaaat
     8401 ttgaacatac cgggcagaat atctggctaa caatgtagcg tccagcccct taccataaaa
     8461 ggaagtaaag agaggtcaag aattatgatt actacccgaa ctgccaggca gtgtggacaa
     8521 gcagactacg gatggttgca ggcccggtat actttttcct ttggacacta cttcgacccg
     8581 aaattgttag gctatgcctc cctgcgtgtg cttaaccagg aagtgctggc cccaggtgcc
     8641 gcctttcagc cgcgaaccta tcccaaagtc gatattttaa atgtgattct ggatggagaa
     8701 gcagagtatc gcgacagcga aggcaatcat gttcaggcca gcgccggtga ggcgttgctg
     8761 ctctctaccc agccgggtgt cagctacagc gaacacaatc tcagcaaaga caaaccgtta
     8821 acacgaatgc agctttggct ggacgcctgc ccgcagcgag agaatccgct gattcaaaaa
     8881 ctggcgctta atatgggcaa gcagcaatta atcgcctcgc cagagggtac gatgggaagc
     8941 ctgcaattac gccagcaagt gtggctgcac catatcgtgc tcgacaaagg cgaaagtgcg
     9001 aatttccagt tgcatgggcc acgcgcgtat ttgcaatcga ttcacgggaa atttcatgcg
     9061 cttacgcatc atgaagagaa agcggcgctg acctgcggtg atggggcgtt tattcgtgac
     9121 gaggctaaca ttacgctggt tgccgattcc ccactgcgcg ctttgctgat agatttgcct
     9181 gtctagttgt ttttacaggg agatgatgat gagtaaaaaa tcggccaaaa agcgccagcc
     9241 agtgaagccc gtggtggcga aagaacctgc tcgcaccgcc aaaaattttg gctatgaaga
     9301 gatgttgagc gagctggaag ccatcgtcgc ggatgctgaa acgcgtttag ccgaggatga
     9361 agctaccgcg taaagacagg ttgccggatg cggcgaaaac gccttatccg gcctacaaaa
     9421 tccaacaaca ttaacgaatt aaacaacccg taggccggat aaggtgcgcc agcatcgcat
     9481 ccggcacaat actcaaaacc tggcgtttat ctggccttgc tcgccataat ctcgataatc
     9541 tgccgatccg tttgctgcat cgaatggctt gctaacgcac acaggttggc aatcgactgc
     9601 tcaacatcat gcgccacgat cccttcattg ccggtcacgg cggtatcatc cagcgccatt
     9661 aacaccgctt tccacgcagc cgaagcactg gtcgaaacct tcatcgcgca gctgttcgac
     9721 gcaccatcgc aaatcatgcc gctgacatcg ccgatcatac tgctgatcgc catcgagata
     9781 gtttcataac gcccatccac cagccatgcc atcccggcgg cggcccccat tgctgcggtc
     9841 gttgcggcac aaagtgcaga caaacgcggt aactggttat ggatgtaaat tgcgctcaaa
     9901 tgagaaagca tcagcgcacg cgccagtcgt tcatcatccg ctccgaagtg ttctgctaca
     9961 accaccacag gcattgttgc ggtgatcccc tggttacccg agccggagtt actcatagcc
    10021 ggaagcgtag cgccgcccat acgcgcatcg gatgccgcgc tggtacgaat cacaatggat
    10081 gaagagagat ctttcgccag caaaccgcgc tcgcactgtt tttccagcgt cgcgccaata
    10141 tgcagccccc acttaccgct caaaccttcc tgcgataacg cacaatttag cttcgcggaa
    10201 tcgagaataa agcggatcgc cgcaaacggg acttcattga cgaacttcag gatctcagcc
    10261 agcgtcgttc tggaaagcac cgtcagcgga gactcttgct cgccctctgc cacacacgcc
    10321 tgctgggtaa acaccacacc attgtgcgtc tcaatacgca caatgttggt atgcccgccg
    10381 acgatggtga cacacgccca cttctcaccg ttccagactt tggcgcgtga gaagaggatt
    10441 tcattgcaag gttcctggat cttaacggag actttccccg ccgccagcag tgctttggca
    10501 tcggcaattg cctgcgcagt tgcgtctttc agcacttcca gcccggcgtt ggcatttcca
    10561 cctaacgccc ccagcgccgc cgcaatcggc agccccacca ttcccgtgcc gggaacggtg
    10621 acgcccagac cgttcttcat cagatttggc gaaacccagg cttctacacg ttcaaccgga
    10681 ccttccagtt ctgccgcagc aaccgccgcc gccagcgcca gtgaaatcgg ttcagtacat
    10741 cccagcgccg gttttacttc ctcctgaacg gcgaggatgt aacgctgcca taacggattt
    10801 aaagtcgaat caaacatatt aaaaacctta aaatttcagg taaatcaaga aaatgccagg
    10861 aacggagaaa cacaaagcaa caaaccagtg acgataatca ggtacagaga catccctttg
    10921 tatttgtgca atgccggtac tttgtaaacc agccacgccg ggatcaggca ccctaccatg
    10981 ccgaaaatcg ggctacagat agaggtgaag ctcaacaccg gtgcgttcag tacgatagcg
    11041 ctccaggcca gcaaaatggc gaaaatcatg atgccgcgct gaacaagatt ttcgttaatc
    11101 ttctcggcag gcatcttgcg acgcaggatg ttcattacga tcccttgcgt tgcttcgcga
    11161 aagcctaaat agacgccaaa gaatgcagtc atgacagcaa agatattgag aatgacgctg
    11221 accactttca cccacgctgc gccgtcaccg ctaataaact gtgcggcaat cgccagcgcg
    11281 gaaatattct gctcgtaggc ttttaccgct tcgtcatgcc ccatcgccag cgtgaacgac
    11341 acggcgtaga aaaagacggt gacaaacaaa atgccaaacg cgatattcat tgcccgcaat
    11401 gctttatgcc gcgccacttc aatagatttt tcccgcgagc gataagagat caccatcggg
    11461 cttaacgtct ggataaacaa aatcgacgtc agggtaaacg gcagcgtaat aatggcgttt
    11521 ttcaccagca gccccagcgg cggtagcgaa ccgacgttat acagatgcca cattcccacc
    11581 atcgacacgc ccagcgccgc gaccaccagc agcttggtca gcaccatgcc ggtcgaaatt
    11641 ttgaataaca atttctcgcc gcgtgaagag atcgccacca gaatgcaaat cagtaccaga
    11701 ccataaaagg gactgtctga cagcaaccct tccgtcacgc cgaaggtatg cagataggaa
    11761 gcactatcgt tggtgatggc ggtggaatag acgaacatcc aaatcaccag cattacgaaa
    11821 tagagcgcac ctaacaggat gccccagttt ttacctaaat aaccgctaat gacactcggg
    11881 taatctttac attctggtga ttctgccagc gtattaataa acaaccgctg aaacagatac
    11941 attgccgggt aaccaatcac cgatgagagc aaaaataccc acaatcccat caaaccgacc
    12001 tgcaccggga gaaaaacaat ccccgcgcca atcgccatcc cgatactcat aatcacccag
    12061 ccggtgtcag tactgtcgaa tttgatcgct tctcgccact cgctctcact cattccagca
    12121 cgacccgccg gggtcgaagc gtctgcaatg acgcctttat tcgatgcgat ttccataatt
    12181 tctcgctcaa tattttgtag ggcttattat ttttttccga gccgcatcaa ggcgatatgc
    12241 ggtctacgtt tttgcaggcg agttattaga ataaagaaat gatacgcgcg aggtcagaga
    12301 aaatcttcac aatcaagact ttgaaataac ggagatggat aagaattttc tactaaatta
    12361 atcgcaagag aatattcatt ctctatttgc ggcagatcac aaaaaaaagg tgcacatttg
    12421 tgcacccaag gatgaaagct gacagcaatg tcagccgcag accactttaa tggccagtcc
    12481 tccgcgtgat gtttcgcggt atttatcgtt catatctttg ccggtttcat acatcgtctc
    12541 gatcacttta tcgagtgaaa cacgcggtgc cgaggtgcgg cgcatcgcca tccacgcggc
    12601 gtttactgct ttcacggcat taatggcatt acgttcaatg cacgggattt gtacctgtcc
    12661 ggcaaccgga tcgcaggtca gcccaaggtt atgctccatc gcgatttccg ccgcattgca
    12721 tacctgcgcc ggactgccgc ccagtagttc agttaaccct gccgccgcca ttgaacaggc
    12781 cacgccaatc tccccctgac agccgacctc cgcgccagag atggaggcgt tcattttata
    12841 cagcgcgcca atagcccccg ctgccagaaa atagcgggca attgaccgct cgtttaccgg
    12901 acgacggaac ttatcgtaat aagccagcac tgccggaata atgccgcacg caccgttagt
    12961 cggtgccgtt accacgcgcc cgccagctgc gttttcttca ctaaccgcca gcgcgtacat
    13021 gttgatccag tcgatgacat tcatcggatc gttagagatg ttatcgctag aaaccagctg
    13081 acgacgcagc gctacggcac gacgcggcac attgagcgga ccaggcagca cgccttcagt
    13141 gttcatcccg cgttcaatac cgtcatgcat cacttgccag atacgggcaa atcctgcgtc
    13201 aatttccgct ttgctgcgca gcgccagctc gttgtgcatc atcaggccag atatagacag
    13261 gccgttgtaa tcacacattt tcagcagttc acctgctgag tggaaatcgt aaggtacgga
    13321 cgtttcgaca tcgtgcgaca ggccgaagtg ttcttcttcg acaataaacc cgccgccgac
    13381 agagtaatag gttttactta atagcgcttc ctgtcccttc caggcagtga tccgcattcc
    13441 gttctcatgg cgaggcaaca tttcgggatg gaagataatg ttctttgcta caggaaaatc
    13501 aacaatatgc gcaccagatg ccactggcag ccgcccgctg cgcgttacta actctataaa
    13561 tgcagggatc tcatcaatga caacatcctg cggactgttt cctgccagtc ccatgataat
    13621 ggcgacatcc gtggcatggc ctttgcccgt cagtgacaac gacccataca gatcgaccac
    13681 aatatggctc gtcgcggtta ataagccgct actttccagc cgatcaataa aactttttcc
    13741 ggcattcatt ggccccacgg tatgcgaact ggagggacca atcccaattt tgaaaatatc
    13801 gaatgcacta atcatatcca caccctcgga ttgccgttca gtgaagtgga gcggaacgac
    13861 cttgcgaccg tcccgctcac gaggctttac gcactacgta ctgcgatagc ttcaatttcc
    13921 agcttcacat ctttcggcaa acgcgcaacc tgcacacagc tccgggtcgg gtaggtcgcc
    13981 tgatgctcat cgaagaactg cttatagact tcattgatgg tggcaaaatc attcagatcg
    14041 gtgatgaaca cggtcatctt gatgatatcg cccacgctca gcccggcagc aaccacgatc
    14101 gctttgacgt tttcgaggct taaacacgcc tgatcttgca catcagccgg gatctcaccg
    14161 gtctgcgggc aaaccggaat ttgcccggag gtgaagacca tgctgcctaa atcaacgccc
    14221 tgaacataag ggccgattgc gcctggggca cgttgcgttt cgataatctt tttcatacat
    14281 cctccggcgt cagagcgcct gggtaaaagt acgtgaaata acatcctgtt gctgttcacg
    14341 ggtcagtgcg ttgaagcgca cggcgtagcc agagacacgg attgtcaggt taggatattt
    14401 ttccgggtgc tcgatggcat ccagcagcat ttcccgattc attacgttga cgttgaggtg
    14461 ttgaccgcct tcgacatccg cttcgtggtg gaaataccca tccagcaggc cgacaaggtt
    14521 ggttttacgt actggatctt ctttgcccag cgccgcagga acgattgaga aggtgtacga
    14581 gatcccatct ttggcgtagg tgaacggcag tttcgccacc gacgtcaatg aggccacggc
    14641 acctttgcgg tcacgaccat gcatcgggtt agcgcccggc gcgaacggtg ttccggcgcg
    14701 acgaccgtcc ggcgtattac cggttttctg gccgtacacc acgttagaag tgatagtcag
    14761 aatcgactgg gtagggacgg cgttgcgata ggttggcagc gctttaattt tcttcataaa
    14821 gcgttcaacc aggtcgcagg caatgctgtc tacgcgctcg tcgttgttgc cgtactgcgg
    14881 atattcaccg tcgatttcaa agtccaccgc caggccgttt tcgtcacgga ttggtttcac
    14941 gcgggcatat ttgatggcag acagggagtc cgtcgccacc gacaggcccg cgatgccgca
    15001 tgccatagtg cgatagacat cacgatcgtg cagcgccatc agcgaagctt cgtagctgta
    15061 cttgtcgtgc atgtagtgaa tgatattcag cgcgctgatg tactgcaccg ccagccagtc
    15121 catgaagtga tcgaggctgt ccatcacttt gtcgtaatcc agcacgtcgt ccatcagcgg
    15181 tgctgttttc ggcccgacct gaatcttcag cttctcgtcc accccgccgt taattgcgta
    15241 cagcagtgtt ttcgccaggt tagcgcgtgc accaaagaac tgcatttgct taccaatcac
    15301 cattgggctg acgcaacaag caattgcgta atcgtcgctg ttgaagtcag tacgcatcag
    15361 atcgtcgttt tcatactgca aggaagaggt gacgatcgac acctgcgcgg catatttttt
    15421 gaaggcaatc ggtaattctt ccgaccaaag aatggtcagg ttaggttccg gtgccggccc
    15481 catagtgtgc agggtgtgca aatagcggaa ggagtttttg gtcaccagcg tacgaccgtc
    15541 cagccccatc ccgccgatca cttccgtcgc ccagattggg tcgccggaga acagcgaatc
    15601 aaattccggc gtacgcaaga agcgcaccat acggatcttc ataatgaagt gatcgatcag
    15661 ttcctgtgcc tgctgctcat tgagtacgcc tgctttaaag tcgcgctcaa tgtagatatc
    15721 gaggaacgac gccgtgcgcc ccagcgacat cgcgccgccg ttctgtgatt tcactgccgc
    15781 cagataagcg aagtagagcc actgcaccgc ttcctgcgca ttctgcgccg ggcgagagat
    15841 atcaaagcca tatttcgccg ccatttcctg aatctgcaac agcgcatgac gatgctctgc
    15901 cagctcctca cgcagacgga tggtggcttc cagatcttcg cctttttcca gacgggactg
    15961 gagatcggca aattgcagtt cgcgttcacg caccagataa ctgatgccat acagcgctac
    16021 gcggcgatag tcaccgataa tgcgcccacg gccatagcca tctggtaaac cggtcagcac
    16081 gccagattta cggcagcgca gcatatccgg tgagtaaaca tcaaatacgc cctggttatg
    16141 ggttttacgc agatcggtaa acagatattc aaattcactg tccatttcac ggccataggc
    16201 gtggaatgaa cttttaatca tattaatgcc accgaacgga tgcagcgcgc gctttaatgg
    16261 cgcatccgtt tgcagaccaa caattttttc cagcggctgg ttaatatatc ccgcatcatg
    16321 agcggtaatt gtggtggcaa tattggtatc gaaatcaacc ggcgcgtggg ttgcgttttc
    16381 gatacggatg ccttccatta ctttttccca caattccgtg gtggcaggcg tcgcttcggc
    16441 gaggaaagat tcatcgcctt catacggtgt atagttatgt tgaataaaat cgcggacatt
    16501 aatttcgttt ttccagtccg tacctttaaa gccaagccat gcgtcggcgt acagcttatc
    16561 gctggtatca atatctatct tcatgaaaaa taatctctct acaatacttc aactaaatta
    16621 tgcaaattct gcgggcgcgt taactttgcc taaatgaatg gcatccaaag caatcatttt
    16681 ttcttcgtta gtcggaataa cggcacaaat gacacgcgca ttttcactgg aaacaattcg
    16741 ctctccaaaa gagttagagc gattattcat ttctgtatca atcactacgc ctaatacagc
    16801 caaatgttcc atgaccagac gacgaattaa acttgaattc tctcctattc cgccggtgaa
    16861 tataattcca tccaggcgac gtaatgaagc tgcgtgtccg gcaatatgac gggcaattcg
    16921 gtgaacaaag gttttaattg ccagttgcgc gcgttcgtga ccttcatgcc aggctttttc
    16981 cagaacacgt aaatccgaag aaagaccgga aatacctaat aatcccgact ctttattcac
    17041 tacgcgttcc aggtcaccca ggctctggtt ggtttggctg gcgacccagg acatcgcacc
    17101 aaagtcgaca tcgccactgc gggttcccat catcaagcct tccagcggcg tcattcccat
    17161 tgaagtatca acactctgac cgttgcgaac cgcgcagatt gacgcgccat tgccaagatg
    17221 cgccacaacc aggccggagt catcttccgc cagattcagc agagaatgtg cgcgctggga
    17281 aacatagcgg tgcgacgtgc cgtggaaacc ataacggcgt acacctaact cttcataata
    17341 tttccacggc aggccgtata aataagcttc cggagccatc gtctggtgga aactggtatc
    17401 aaataccgcc acctgagtta cgcccggaaa taattgctgc gccgattcaa taccacttaa
    17461 attggcgtaa ttatgcaggg gtgccagtgg agaaacgcga cggatattat caatgacttc
    17521 atcggtaata atggcggact cggtaaaaat actgccgccg tgagcgatgc ggtggccaat
    17581 taaggccaca ctgtcattta aattccgttt ttccagttca aatgcaattg ccttcaatgc
    17641 accttcgtag ctgtggtgag ccagcggtgc tggctctccc ccatttacgg ataagaatgc
    17701 attttccgag ttaataccgt cggcaatacc tgacattaat acttcacagt cgctggcatc
    17761 gagcacggaa aacttaatcg aagacgaacc acagttaata accaaaacaa ccggaaattc
    17821 attcatctct tttctcatcc tgagttacgg attaaaacag tttgtatacg atgttcagga
    17881 tggtcagcag accaatcacg gtaacaaaca cgttatccag acgaccacgg tatttcgcca
    17941 gagacggcgc tttacggatg gcatacatcg gcaacaggca cagcagggat gcgataatcg
    18001 gtgcgcccat ggcttcaatc aggtcgagga tgttcgggtt ggcgtaggca acaacccagg
    18061 tggagcccat gatgaagatc atgctgatag tgttcagttt acccagcgac actttagttt
    18121 tgtcgccttt ataaccaaac ttcaggacca ggccattcag accttccagc gttcccagat
    18181 agtgaccgaa gaaagatttg aagatagcca cgagtgcgat gatggaagcc gcatattcca
    18241 gtgtaatcgc gaacgttgtt ttggtaccgg tcatggacgc aaagtggtta gccagataag
    18301 aaagcactgg aatattctgc gctttggctt ccgccatgtt ggccggagac agcgtaaata
    18361 ggcagctaaa ggcaaagaac atcaccactg caaccatcag catgctggca cgagaaatga
    18421 tttgggaaca tttacgttcg gtgaagtcgc gaccgaagtc tttctcatac tcttcacgtt
    18481 tagaaaccac gaaggaagag acgattggcg agaaattaaa ggagaaaacc atgatggaaa
    18541 tccccagcca gacggtgatc aggataccgt catgaccggt taacgacagc gaaccgaggt
    18601 caacctggtc gataactgca gagttccagt aagggatcag cgacagagaa atcagcacca
    18661 ggctggcgat aaacggccat accaggtagc tcatcacttt aaccatcaga tccttaccaa
    18721 accagatgac gaaagccatc agcagcagca ggaacagcgc cacaaagccg cgattcagcg
    18781 gtgcaaagcc gagctggttt tcccagaacg tcataaaggt attggtaata gtaacgccat
    18841 aaatccacag cagtgggcaa atcgcgaaga agtacaggaa cgtgataacc acgccgccag
    18901 ttttaccaaa atgctcttcc accgtttccg taatgttgcc ggaagggtta gagccagaaa
    18961 gacatagacg cgccagcgcc cggtggcaat aaaatgcgat ggggtatgcc aataccaaca
    19021 tcagaagaat cgggatcagt ccgccaaaac ctgcgcggat agggaagaac agcaccccgg
    19081 cgccgattgc cgtaccaaac aagcctaacg tccatgtggt atctgattta cgccaggacg
    19141 attgttttgt ctggctggat acaatgctat ctgaagtact catatcctat cctcaacgaa
    19201 ttaattaagc gtcaacgaaa ccggtgattt gagagacgcg agaaagatcg atattgccgc
    19261 cggaaataat actgacggtt tttctgtttt gaatatattg gtctaattta ccgcttaata
    19321 atgcagcaca tgccagagcg cctgcgcctt cggtgacgac tttattgcgc tgaattaagg
    19381 caatcatact gtttctgatt tcgtcttcgc tgaccagcac gatgtcatcg actaattcac
    19441 gaacgatttc gtaagttaaa ttacccgggc gggagacatc acaaccatcc gccagggtgc
    19501 cggtagttcg gtgcgtggtt atttctccgg agtggaaaga agccgccatg ccgtgaacgt
    19561 tttcagactg tacgccaata acacgaatgg tcgggttaat agatttaatt gccaccgcaa
    19621 taccagcaat taaaccgcca ccaccaattg gcacaatcac gttatcgaca tcatagagat
    19681 cttccataat ttccagacca atcgttccct ggccagcaat cactttcgga tcatcgtaag
    19741 gtgggataaa aatacggcct tccatttcga caatttcgct cactttagcg atagtgtcgt
    19801 tgaagttatc accatgcaga acgacttctg cggagtagtc gcacgttgcc gctactttgg
    19861 attttggcgc accttttggc atcaccactt taccgtcgat acccagcatc gcgcaggaga
    19921 gcgaaacccc ttgcgcatgg ttgcccgcag aacaggccac cacgcctttg cgtttttccg
    19981 catcggtcag tgaacttaat ttattaaatg cgccacgaat tttaaatgaa cccgtacgct
    20041 gcatattttc aaacttcagg aatatttcac ctttgcaacg ttcactaaaa tagttggagc
    20101 gaggcatgcc tgttttataa attcgcccag ccagtcgttg tttcgcttca ataatatcat
    20161 caatagcaat cggcagatcg tatgtaatat gcattataaa acctcttcgc ctgaattaaa
    20221 tatgtaggta accgtaaccg acacctgcaa aacagacagg tggattattt atgtttaaat
    20281 aaaaacaaat aactaaccaa cttctattaa ttgccttcgt ctacacccat tataagatga
    20341 atactctttg gctaattcca ccaaaaccga tgctgctttt ttaatacgat aatttttcga
    20401 ccataccgcg gcatattgtg ccacaggtaa tgtttcttca accggaatag taataaattg
    20461 attagaacca aaaggtgacg tcatatcaca aggaattaca gttaagaaat cagcattgag
    20521 aacaagatta taaattgtca cgactgagtc ggttttaacg atgttttcaa tactgatgcc
    20581 atttctttgt aacgtagtaa gcagttcgct gtagtacccc atattagttt gtggcaacac
    20641 ccactgttcg ttcttcaacg actccagcgt ggtggtgccg gtgcatgttc gggacttact
    20701 ggctaccagc acaaactcgg actcgaacag cggctcaaca tgtaaatcct gaagcttcat
    20761 ttctgcactt aatgtaccaa tcgcaaaatc cagtcgaccg tcgcggattg ccggtaagaa
    20821 cgaagacagt tgcgcttcat acatagaaac ctgcgctttc gggaacacct ctttgaactt
    20881 gttgatcatc cctgacataa aagtaaaacc aatcaatgaa ggaaaaccaa atgagacttc
    20941 caccaccgcc tcagaagaca taccgcttat ctcattaacc atatttttca tttcacgggt
    21001 aatggattcg gaacgggaga gtaacaattg accggcaggt gttaatgtta caccggtatt
    21061 tttccgcacc actaattcca caccaaaata atcttcaata tcgttaatga ttttactgac
    21121 ggccggttga gttaacccta attcttttgc agccgagccg atagagccac ttctaatgac
    21181 ttcctgaaag actaccaggt gctgcgtttt cggaagaaga atagtgctca taacgaccta
    21241 cgttaattac ctcattgacg gcatgaagtg tatcaaaatg aaatgaacag gatatgtgcg
    21301 accactcaca aattaacttt caatactttc cagggtatcg tcattacaat taaatacctt
    21361 taaatatcaa caagttaaag cataaaaatc agcataaacc ctgatttttg tcaaaaaaaa
    21421 tgacggggat aaccatattt tatggcgata acatcattcg ttatgtggtt aatacaaaaa
    21481 aggggctgag accatatttc aagcaattac cggataatta ccaggcaaaa tttgagttaa
    21541 atttaatgag caagttctca aattttatta taaataaacc attttcagcg ataaatactg
    21601 cggcacgtca cattttttca cgctatttgt tggagaacaa acatttattt tatcaatatt
    21661 ttaaaatttc gaatacaggt attgatcatc tcgaacaatt gattaacgtc aactttttct
    21721 cttctgacag gacgtcattt tgtgaatgca atcgttttcc ataaattctt ctcccctcat
    21781 aggcgacgaa tagcattttg tgttgaggat cacaaaacga ataattgctg atcgccgcga
    21841 taaggtcaga caaagacaac aagggaaatt ttcacagagc ttttgatcgt cgtaggccac
    21901 agaatgttgc ttcgtttact gtaacgccgg gtaaatgagc gttttttgat agtgcgaaag
    21961 aaccctgcga ggaaaataag catctattat tgtttgtcat gacgacaagg caggcatcaa
    22021 tgaaaggctt cccaattgca catatttttc acccttcaat cccgccaatg cacgcagtgg
    22081 ttaacaatca caatagaaat attgattatt ggacggtaaa aagaaagttt gcagaaatt
//
LOCUS       DADMKF010000059        21998 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.060, whole genome shotgun sequence.
ACCESSION   DADMKF010000059 DADMKF010000000
VERSION     DADMKF010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21998
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.060"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(<1..988)
                     /locus_tag="JNC24_002071"
     CDS             complement(<1..988)
                     /locus_tag="JNC24_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HAY9158816.1"
                     /translation="MENKSARAKVQAFGGFLTAMVIPNIGAFIAWGFITALFIPTGWL
                     PNEHFAKIVGPMITYLLPVMIGSTGGHLVGGKRGAVMGGIGTIGVIVGAEIPMFLGSM
                     IMGPLGGLVIKYVDKALEKRIPAGFEMVINNFSLGIAGMLLCLLGFEVIGPAVLIANT
                     LVKECIEALVHAGYLPLLSVINEPAKVLFLNNAIDQGVYYPLGMQQASVNGKSIFFMV
                     ASNPGPGLGLLLAFTLFGKGMSKRSAPGAMIIHFLGGIHELYFPYVLMKPLTIIAMIA
                     GGMSGTWVFNLLDGGLVAGPSPGSIFAYLALTPKGSFLATIAGVTVATLVSFA"
     gene            complement(1016..1459)
                     /gene="cmtB"
                     /locus_tag="JNC24_002072"
     CDS             complement(1016..1459)
                     /gene="cmtB"
                     /locus_tag="JNC24_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IIA"
                     /protein_id="HAY9158817.1"
                     /translation="MRLSDYFPESSISVIHSAKDWQEAIDFSMVSLLDKNYISENYIQ
                     AIKDSTINNGPYYILAPGVAMPHARPECGALKTGMSLTLLEQGVYFPGNDEPIKLLIG
                     LSAADADSHIGAIQALSELLCEEEILEQLLTAASEKQLADIISRG"
     gene            complement(1773..3764)
                     /gene="tkt"
                     /locus_tag="JNC24_002073"
     CDS             complement(1773..3764)
                     /gene="tkt"
                     /locus_tag="JNC24_002073"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HAY9158818.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIIGMTTFGESAPAELLFEEFGFTVDNVVAKAKELL"
     gene            4042..4800
                     /locus_tag="JNC24_002074"
     CDS             4042..4800
                     /locus_tag="JNC24_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HAY9158819.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLRQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRMSADGIK"
     gene            complement(4943..5863)
                     /gene="speB"
                     /locus_tag="JNC24_002075"
     CDS             complement(4943..5863)
                     /gene="speB"
                     /locus_tag="JNC24_002075"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HAY9158820.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLTFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(6001..7977)
                     /gene="speA"
                     /locus_tag="JNC24_002076"
     CDS             complement(6001..7977)
                     /gene="speA"
                     /locus_tag="JNC24_002076"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HAY9158821.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(7986..8117)
                     /gene="yqgB"
                     /locus_tag="JNC24_002077"
     CDS             complement(7986..8117)
                     /gene="yqgB"
                     /locus_tag="JNC24_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HAY9158822.1"
                     /translation="MKKKPVAQLERQHSLLENPCAYGLLSQFQAAIVVNCFTLNKII"
     gene            8253..8468
                     /locus_tag="JNC24_002078"
     CDS             8253..8468
                     /locus_tag="JNC24_002078"
                     /inference="COORDINATES: protein motif:HMM:NF037550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9158823.1"
                     /translation="MGITSAGMQSRDAECGERIFTRTVRQVKQQTTVHYFVSPPRPPV
                     KTNPQAKTLISTRLEVATRKKRRVLFI"
     gene            8772..9926
                     /gene="metK"
                     /locus_tag="JNC24_002079"
     CDS             8772..9926
                     /gene="metK"
                     /locus_tag="JNC24_002079"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HAY9158824.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
     gene            10362..11756
                     /locus_tag="JNC24_002080"
     CDS             10362..11756
                     /locus_tag="JNC24_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HAY9158825.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGSGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            11833..12330
                     /locus_tag="JNC24_002081"
     CDS             11833..12330
                     /locus_tag="JNC24_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HAY9158826.1"
                     /translation="MKTSRLPIAIQQAVMRGLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAVYRCVHCGE
                     QLVAK"
     gene            12425..13132
                     /gene="endA"
                     /locus_tag="JNC24_002082"
     CDS             12425..13132
                     /gene="endA"
                     /locus_tag="JNC24_002082"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HAY9158827.1"
                     /translation="MYRYLSIAAVVLSAAFSGPALAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMYNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            13212..13943
                     /gene="rsmE"
                     /locus_tag="JNC24_002083"
     CDS             13212..13943
                     /gene="rsmE"
                     /locus_tag="JNC24_002083"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311849.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HAY9158828.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGKIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            13956..14906
                     /gene="gshB"
                     /locus_tag="JNC24_002084"
     CDS             13956..14906
                     /gene="gshB"
                     /locus_tag="JNC24_002084"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HAY9158829.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTENGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            14943..15578
                     /locus_tag="JNC24_002085"
     CDS             14943..15578
                     /locus_tag="JNC24_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708713.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HAY9158830.1"
                     /translation="MTAPFFPRILGAVAFLNQETEPLTMNLQHHFLIAMPALQDPIFR
                     RSVVYICEHNTNGAMGIIVNKPLENLKIEGILEKLKITPEPRDESIRLDKPVMLGGPL
                     AEDRGFILHTPPSNFASSIRISDNTVMTTSRDVLETLGTDKQPSDVLVALGYASWEKG
                     QLEQEILDNAWLTAPADLNILFKTPIADRWREAAKLIGVDILTMPGVAGHA"
     gene            15578..15994
                     /gene="ruvX"
                     /locus_tag="JNC24_002086"
     CDS             15578..15994
                     /gene="ruvX"
                     /locus_tag="JNC24_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HAY9158831.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(16169..17149)
                     /locus_tag="JNC24_002087"
     CDS             complement(16169..17149)
                     /locus_tag="JNC24_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HAY9158832.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGLMEAAPFEAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRHGISLALRLLPSHCPQ
                     LEQLGAPTVLPELLKSENGLILVTGATGSSKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YIYASQRCLIQKREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSFQQRVGEGRL"
     gene            17167..17871
                     /gene="yggS"
                     /locus_tag="JNC24_002088"
     CDS             17167..17871
                     /gene="yggS"
                     /locus_tag="JNC24_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HAY9158833.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            17889..18455
                     /gene="yggT"
                     /locus_tag="JNC24_002089"
     CDS             17889..18455
                     /gene="yggT"
                     /locus_tag="JNC24_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HAY9158834.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVT
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFKVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQATGNMLLPGLWMAL"
     gene            18452..18742
                     /locus_tag="JNC24_002090"
     CDS             18452..18742
                     /locus_tag="JNC24_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggU family protein"
                     /protein_id="HAY9158835.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQGNSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            18750..19343
                     /locus_tag="JNC24_002091"
     CDS             18750..19343
                     /locus_tag="JNC24_002091"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HAY9158836.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARYAAKVTGLPAIADDSGLAVDALGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            19336..20472
                     /gene="hemW"
                     /locus_tag="JNC24_002092"
     CDS             19336..20472
                     /gene="hemW"
                     /locus_tag="JNC24_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HAY9158837.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     CDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFIAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
     gene            20783..21769
                     /locus_tag="JNC24_002093"
                     /pseudo
     CDS             20783..21769
                     /locus_tag="JNC24_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014839537.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
     gene            21814..>21998
                     /locus_tag="JNC24_002094"
     CDS             21814..>21998
                     /locus_tag="JNC24_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HAY9158838.1"
                     /translation="MIFNRLKLAVDRVIAAFSVAVMLALVVCVVWQVFSRYVLNQPST
                     LTDELARFLMIWVGLLGA"
BASE COUNT         5443 a         5716 c         5596 g         5243 t
ORIGIN      
        1 tagcaaagga caccagggta gctaccgtaa caccggcaat tgtcgccagg aacgagcctt
       61 tcggcgtcag tgccaggtaa gcaaagatag accccggact tgggccagcc accagaccac
      121 cgtccagtaa gttaaacacc caggtgccag acataccgcc cgcaatcatg gcaataatgg
      181 tcagcggctt catcagcaca tacgggaaat acagttcgtg gatcccaccg aggaagtgaa
      241 taatcatcgc cccgggcgca gaacgtttac tcatcccttt accaaacaag gtaaacgcca
      301 gcagcagccc taagccaggc ccaggattgg acgccaccat aaagaagatg gatttaccgt
      361 taaccgaagc ctgttgcatt cccagcggat aatagacgcc ctgatcgatc gcattattga
      421 ggaaaagcac tttcgccggt tcattgatga ctgacagcag aggcagataa cccgcatgta
      481 ccagcgcctc aatacactct ttgacgagag tattggcaat taacaccgcc gggccgataa
      541 cttcaaaacc cagcagacaa aggagcatcc ccgcgatacc taatgagaag ttattgataa
      601 ccatctcaaa accggcaggt atgcgttttt ccagtgcctt atcaacatat tttatgacca
      661 gcccaccgag cggccccata atcattgagc caaggaacat cgggatctct gcgccgacga
      721 tcacaccaat agtacctatt ccgcccatga ccgcgccgcg tttaccgccg accagatgac
      781 caccggtaga accaatcatc acgggcaata aataggtaat catcgggccg acaattttgg
      841 cgaaatgttc gttaggcagc caaccggtgg gaataaataa cgcagtaata aaaccccagg
      901 caataaaagc accaatattg gggatgacca ttgccgtcaa aaagccccca aaagcctgga
      961 cctttgcacg agcagacttg ttttccataa tattatcctg tagaggagag agtaattatc
     1021 cgcggctgat tatgtccgct aattgttttt ctgatgctgc tgttaagagt tgttcgagta
     1081 tttcttcttc gcacagtaat tcacttaacg cctgaatagc gccaatgtgc gaatcggcat
     1141 cggcagcaga aaggccgatg agtaatttta ttggttcgtc attccccgga aaataaacac
     1201 cttgttcaag taatgtcaaa gacatcccgg ttttaagcgc cccacattcc ggtcgcgcat
     1261 gaggcattgc cacgcctggt gcgagaatat aataagggcc attgttaatg gtggaatctt
     1321 taattgcctg aatgtaattc tcgctgatat agtttttatc cagcaatgat accatcgaga
     1381 aatcgatagc ttcctgccaa tcttttgctg aatgtataac tgagattgat gattctggaa
     1441 aatagtcact aagccgcata aattatcctt attttatttt tagggtaccg tccggcggta
     1501 aaaataaaat actcgtgcgg tttaatgaaa gcaattgagc agggaaaccg gtgttttgaa
     1561 aacagtgaat tattaatttc ttatataaca ttgagttata gatataacaa caaatgtcaa
     1621 tacgcatatc gtgatgcata ttacataatt gatatttatt gcatataaat atttgtgatc
     1681 tacaacacgc tttatctatt gcttgtccgc aaacggacat atcaaggtaa taaaaaaggt
     1741 cgccgaagcg acctttttac ccgaaatgct aattacagca gttcttttgc tttcgcaacg
     1801 acgttgtcga cggtgaagcc gaactcttcg aacagcaact ctgccggagc tgactcaccg
     1861 aaggtggtca taccgatgat agcgccattc aggccaacat acttgtacca gtagtcagca
     1921 atacccgctt ctaccgcaac gcgtgcagta accgctttcg gcagtacgga ttcacggtaa
     1981 gccgcatcct gcttgtcgaa tgcatcggta gacggcatgg aaaccacgcg cgccttcacg
     2041 ccttcggcag tcagtttttc gtaggcggca acagccagtt caacttccga accggtcgcg
     2101 atgaagatca gttccggctg accagcacag tctttcagca cataaccacc gcgcgcgatg
     2161 tttgccagtt gctcttcagt acgttcctgc tgcgccagat tctgacggga gaggataagc
     2221 gcggtcggac cgtcctgacg ctcaacaccg tatttccacg cgaccgcgga ttcaacctgg
     2281 tcacacggac gccatgtaga catgttcggg gtcacgcgca gagaagcgac ctgctcaacc
     2341 ggctggtgag tcgggccgtc ttcgcccaga ccgatggagt cgtgggtgta aaccatcacc
     2401 tgacgctgtt tcatcagcgc agccatacgt acagcgttac gtgcgtattc gacaaacatc
     2461 aggaaggtgg aggtgtacgg caggaagcca ccgtgcaggg agataccgtt agcaatcgcg
     2521 gtcataccga actcgcgaac accgtagtgg atgtagttac ccgcagcatc ttcgttgatt
     2581 gctttagaac cagaccacag ggtcaggtta gacggtgcca ggtcagcgga gccgccgagg
     2641 aattccggca acagcgggcc gaacgcttca atagcattct gagacgcttt acggctggcg
     2701 attttcgccg gattagcctg cagtttagcg ataaactctt tcgctttggc gtcgaagtca
     2761 gacggcattt cgcctttcat acggcgggta aattcagccg cttcctgcgg ataagctttc
     2821 gcgtaagccg caaacttctc attccatgca gattctttcg cctggcctgc ttctttcgca
     2881 tcccactgcg catagatttc agacgggatt tcgaacggtg cgtatttcca gcccagctgt
     2941 tcgcgggtca gagcgatttc tgcatcaccc agcggcgcac cgtgggagtc gtgagtaccg
     3001 gctttgttcg gggaaccgaa accgatgatg gttttgcaca tcagcaggga cggtttgtca
     3061 gtcactgcgc gcgcttcttc tactgcgcgt ttgatggatg ctgcgtcatg accgtcgatg
     3121 tcgcgaataa cgtgccagcc gtaagcttcg aaacgcattg cggtgtcgtc ggtgaaccag
     3181 ccttcaacgt gaccatcaat agagataccg ttgtcatcgt agaacgcaat cagtttaccc
     3241 agcttcagcg tacccgccag agagcaaact tcgtgggaga tgccttccat catgcagccg
     3301 tcgcccatga aggcgtaggt gtagtggtcg acaatgtcgt ggcccggacg gttaaactgc
     3361 gccgccagcg ttttttctgc aatcgccata ccgactgcgt tggcaatacc ctgacccagc
     3421 ggaccggtgg tggtttccac accagcggta taacctactt ccgggtggcc cggagtttta
     3481 gagtgcagct gacggaagtt tttcagttct tccatcggca gatcgtaacc ggtgaggtgc
     3541 agcaggctgt agatcagcat ggagccgtgg ccgttggaca gcacgaagcg gtcacggtca
     3601 gcccaggacg gattctgcgg gttgtgtttc aggaaatcac gccacaggac ttcggcaatg
     3661 tcagccatac ccataggggc acccgggtga ccggatttgg ctttctgtac tgcgtccatg
     3721 ctcagcgcac gaatagcatt ggcaagctct ttacgtgagg acattttgac tccagatcgg
     3781 atgatgaagg gcacgccctt aacgacttga cgacagcgcg ttttgggcta cgccggaaaa
     3841 tttgccaaca atttaccgca agccgcgcgt catgtacatg gaacatcctt ttgccgcttc
     3901 agaaatctct ggatcatgct cgcatgttgc gcaatctact cgcccgtccg ctgcgctttt
     3961 ccttatactg agactgagcg tcgattcacc tgcaaacggc gcatttttag aataatcctg
     4021 accttgtgcg gaagagaaaa catgaaaatt cgcgccttat tggtagcaat gagcgtggca
     4081 acggtactga ctggttgcca gaatatggac tccaacggac tgctctcatc aggagcggaa
     4141 gcttttcagg cttacagttt gagtgatgcg caggtgaaaa ccctgagcga tcaggcatgt
     4201 caggagatgg acagcaaggc gacgattgcg ccagccaata gcgaatacgc taaacgtctg
     4261 acaactattg ccaatgcgct aggcaacaat atcaacggtc agccggtgaa ttacaaagtg
     4321 tatatggcga aggatgtgaa cgcctttgca atggctaacg gctgtatccg cgtctatagc
     4381 gggctgatgg atatgatgac agataacgaa gtcgaagcgg tgatcggtca cgagatgggg
     4441 cacgtggcgt taggccatgt gaagaaaggg atgcaggtgg cgcttggtac aaatgccgtg
     4501 cgagtagctg cggcctctgc gggcgggatt gtcggcagtt tatctcaatc acaacttggt
     4561 gatctgggcg agaaattagt caattcgcaa ttctcccagc gccaggaagc agaagccgat
     4621 gattattctt acgatcttct gcgccaacgc ggcatcagcc cggcaggtct tgccaccagc
     4681 tttgaaaaac tggcgaaact ggaagaaggc cgccaaagct caatgtttga cgaccatccg
     4741 gcatccgccg aacgcgccca gcatattcgc gatcgcatga gcgcggatgg gattaagtaa
     4801 agcctggtgg tgtcgaacgt aggtcagata aggcgttcac gccgcatctg acatcaatgg
     4861 cacatttgtt accctgtgcg cattgccgga tgcgatgctg actgcatctt atccggccta
     4921 cgggtggcat cgcatctggt gcttactcgc cctttttcgc cgcttggata tacagcatct
     4981 ccagcgccag cgtcgccgca gccagagcgg tgatttccga ctgatcgtat gccggagcca
     5041 cttccactac gtccatccca acaatgttga gatctttcag gccgcgtacc agtttaatag
     5101 cgcgatcgga ggtcaggccg ccaatcactg gcgtaccggt gcctggtgca aaagcaggat
     5161 ccaggcagtc gatatcaaaa gtcaggtaaa ccggcatatc acccacaatc tgtttcactt
     5221 gggcgataac gtcatccacg ctgcgatcgt tcacctggca ggcgtccagc acggtaaagc
     5281 cgttgtcttt atcaaactcg gtacgaatac caatctgcac ggaatgattc gggtcgatca
     5341 gaccttcttt cggcgcggta tagaacatag tgccgtggtc aaattcacaa ccgttcgcat
     5401 aggtatcggt gtgggcgtca aagtgtacca gcgccatttt gccgaaatgc ttcgcatgag
     5461 cacgcagcag cggcagcgta acaaagtggt caccaccgaa ggtgagcata cgcttaccgg
     5521 cagccagcag cttctcggcg tgcgcctgca gcttttcgct catctcacgg gcatcgccaa
     5581 aggcatatac cagatcgccg cagtccacga cgttcagacg ctcacgcatg tcgaaattcc
     5641 acgggaagcg gttgtgttcc caggccagat tcgtcgaaac ctgacggatc gctgccggac
     5701 cgtggcgacc acccgcacga ccagaagtgg ccatatcgaa cggcacgcca gtaatcaccc
     5761 agtctgcatc gctgtcatac ggctggaagt tcatcggcag gcgtaaaaaa ccaaaggcat
     5821 tggaaaccag tgagttatcg tattgatgac ctaaggtgct catggatgga ctcctattac
     5881 aaagtcgata taaaaaaacc cttccgcgtc gttaggcccg acgaggaagg gttggatttg
     5941 tcacaataaa ttgtggcgga ttatcaccgc taaattaagc ggattcaagt aacacaggac
     6001 ttactcatct tcaagataag tataaccgta caaacctgcc tcgaactctt caaggaactg
     6061 ttgttgcagt tcagcatcaa gatcggtttt cttcacttga tcgcggaact gggttaacag
     6121 cgttttcgga tcgagctgta catattgcag catgtccgcc acggtatcgc cttcgtcaga
     6181 cagttctact tctacgctac cgtcagggaa cacgaacacg tcaaccgctt cggtatcacc
     6241 gaacaggttg tgcatattgc caaggatctc ctgatatgcg ccgaccataa agaaaccgag
     6301 catcggcgga ttctctggat cgtactccgg cattggcatt gtcgtggcaa taccatcacc
     6361 atcaatatag tggtcgatag caccgtcaga gtcacaggta atatccagca gcacagcgcg
     6421 acgttccggc acttgatcca gcccttccag cggcagaacc gggaacaact ggtcgatccc
     6481 ccatgcgtcc ggcattgact ggaacagcga gaaattgacg tacattttgt ccgccatacg
     6541 ttcctgcagt tcgtcgatta tcggacgatg cgcacggttt tgcggatcca gttgcttctg
     6601 cacttcatgg cacatgctca gatagagttg ttctgcccat gcacgttctt gcaggctaaa
     6661 ggtgccggaa gagtagccga tatgaatgtc gtgcagatcc atctggctgt cgtgtaacca
     6721 ttcacgcaaa gaacggcgag ttcccggttc gtgcatttcc tgccaggttt cccacatgct
     6781 ttgcagcgcg cgcggcgcat cttctgcggg cgcggtcggc accgtatatt cgttacgttc
     6841 cacgccgatg atattcgaca ccagcacggt atggtgcgca gtcaccgcac ggcccgattc
     6901 ggtgattacc gtcggatgcg gcagaccatt ttcttcacac gcatcgccaa tcgcccagat
     6961 gatgttgttg gcgtattcat tgaggccata gtttacggag cagtcagact gcgaacgggt
     7021 gccttcatag tccacgccca gaccgccgcc gacgtcgaag cactgaatat tgacgcccag
     7081 cttgtgcagt tccacataga aacgcgcgga ttcacgaacg cccgtggcga tatcgcgaat
     7141 attcgccatc tgcgaaccga ggtggaagtg cagtagttgc aggctgtcga gacgcccggc
     7201 ttcacgcagg gtttcaacca gttgcagtac ctgagtcgca gccaggccga acttcgattt
     7261 ttccccgccg gaggactgcc atttacccga gccctgcgaa gccagacgtg cacgcacgcc
     7321 cagacgagga acgacattca gacgttctgc ttcatccagc acaatggcga tttctgacat
     7381 cttctcaatg accagataga ccttgtgccc catcttctcg ccaattaatg ccaggcggat
     7441 atattcgcgg tctttataac cgttgcagac gatgacgcta cgggtcatgc cagcatgtgc
     7501 cagtactgcc atcaactcgg ctttggaacc ggcttccaga cccagcggtt cgcccgaatg
     7561 aatcagggac tcaatcacgc ggcggtgctg gttaactttg atcggataaa caaggaagta
     7621 atcgccgtta tagccgtagg attccctcgc acgtttgaac gcggcgttaa tggaacgcaa
     7681 acggtgctgc aggatctgtg ggaaacagaa cagtgcaggc agacgctgac cctgtgcttc
     7741 acgagttttc actaactgcg cgagatcgac gcgagcttcc gggacgtccg ggtccgggca
     7801 cacgctgatg tggcccagct cgttaacgtc atagtagtta ttgccccacc aggcaatatt
     7861 gtaagtacgc agcatcttgc tggcttcctg ggagctcatt gcaacctcct gcatggagcg
     7921 tagtacaccg tgttcgcccg ctgacgaagg caaacccata gacatgtcgt cagacatagc
     7981 gaacctcaaa ttattttatt aagtgtaaaa cagttaacga ctatcgcagc ctggaactgc
     8041 gataacaacc cataagcaca tggattttcc agcagtgaat gctgacgctc caactgcgca
     8101 accggtttct ttttcataac attattaagc acataaccga acgtaagtgt gacagttcgg
     8161 cgaaaccacg agaaaactct tgtttttaca agagcgccct tgttcagtcc tcagtaactg
     8221 taaccagctc ttgaatcctg agaagcgccg agatgggtat aacatcggca ggtatgcaaa
     8281 gcagagatgc agagtgcggg gaacgaatct tcaccagaac ggtgagacag gttaagcagc
     8341 agacaacggt tcattatttc gtatcacctc cacggccgcc tgttaagacg aacccacaag
     8401 ccaaaactct gatttcaacc cggctggaag tggcaacacg aaagaaacgt cgtgtgcttt
     8461 ttatttaagc cgcgcgccgc gttttatacc ccacaatggc agaaaattgc aaaagataaa
     8521 tacgcagaat gccggcattg tcaggaaaaa tttccagcca cgtttttaac agaatgagac
     8581 acgattcaaa aaaaagtgga aatcgggtga agaattgacc taaaatagcc atccagatgt
     8641 taatccatcc ataccgatta acactcagac tgccagtgtt tttaacctgc agagtcgtgg
     8701 taggatccgc taccacagaa aatccacaca acagtttgag ctaaccaaat tctctttagg
     8761 tgatattaaa tatggcaaaa caccttttta cgtccgagtc cgtctctgaa gggcatcctg
     8821 acaaaattgc tgaccaaatc tctgatgccg ttttagacgc gatcctcgaa caggatccga
     8881 aagcacgcgt tgcttgcgaa acctacgtaa aaaccggcat ggttttagtt ggcggcgaaa
     8941 tcaccaccag cgcctgggta gacatcgaag agatcacccg taacaccgtt cgcgaaattg
     9001 gctatgtgca ttccgacatg ggctttgacg ctaactcctg tgcggttctg agcgctatcg
     9061 gcaaacagtc tcctgacatc aaccagggcg ttgaccgtgc cgatccgctg gaacagggcg
     9121 cgggtgacca gggtctgatg tttggctacg caactaatga aaccgacgtg ctgatgccag
     9181 cacctatcac ctatgcacac cgtctggtac agcgtcaggc tgaagtgcgt aaaaacggca
     9241 ctctgccgtg gctgcgcccg gacgcgaaaa gccaggtgac tttccagtat gacgacggca
     9301 aaatcgttgg tatcgatgct gtcgtgcttt ccactcagca ctctgaagag atcgaccaga
     9361 aatcgctgca agaagcggta atggaagaga tcatcaagcc gattctgccc gctgaatggc
     9421 tgacttctgc caccaaattc ttcatcaacc cgaccggtcg ttttgttatc ggtggcccga
     9481 tgggtgactg cggtctgact ggtcgtaaaa ttatcgttga tacctacggc ggcatggcac
     9541 gtcacggtgg cggagcattc tctggtaaag atccatcaaa agtggaccgt tccgcagcct
     9601 acgcagcacg ttatgtcgcg aaaaacatcg ttgctgctgg cctggccgat cgttgtgaaa
     9661 ttcaggtttc ctacgcaatc ggcgtggctg aaccgacctc cattatggta gaaactttcg
     9721 gtactgagaa agtgccttct gaacaactga ctctgctggt acgtgagttc ttcgacctgc
     9781 gcccatacgg tctgattcag atgctggatc tgctgcaccc gatctacaaa gaaaccgcag
     9841 catacggtca ctttggtcgt gaacatttcc cgtgggaaaa aaccgacaaa gcgcagctgc
     9901 tgcgcgatgc tgccggtctg aagtaatctt tcttcacctg ctttcaaagg ccagcctcgc
     9961 gctggccttt ttcttttacc tcaccgcggt ggataggcat tcacgccgca tccggcaaaa
    10021 agctgcccgc acaataacat cattcttcct caacacgttt tatcgcaaat tatcatcact
    10081 actgaaaccg attacaccaa taacaccagc caaatatttg taatattttc atattattat
    10141 tcagttttca cagttgttac atttcttttc agtaaagtct taattgcaga taacagcgtt
    10201 taatctatga tgatataact caattatttt catgcactta aaccataact aagataaatg
    10261 ttagtgtaag cgattacact gatgtgattt gcttcacatc tttttacgtc gtactcacct
    10321 atcttaattc acaataaaaa ataaccatat tggagggcat catgcctgac gctaaaaaac
    10381 aggggcggtc aaacaaggca atgacgtttt tcgtctgctt ccttgccgct ctggcgggat
    10441 tactctttgg cctggatatc ggtgtaattg ctggcgcact gccgtttatt gcagatgaat
    10501 tccagattac ttcgcacacg caagaatggg tcgtaagctc catgatgttc ggtgcggcag
    10561 tcggtgcggt gggcagcggc tggctctcct ttaaactcgg gcgcaaaaag agcctgatga
    10621 tcggcgcaat tttgtttgtt gccggttcgc tgttctctgc ggctgcgcca aacgttgaag
    10681 tactgattct ttcccgcgtt ctactggggc tggcggtggg tgtggcctct tataccgcac
    10741 cgctgtacct ctctgaaatt gcgccggaaa aaattcgtgg cagtatgatc tcgatgtatc
    10801 agttgatgat cactatcggg atcctcggtg cttatctttc tgataccgcc ttcagctaca
    10861 ccggtgcatg gcgctggatg ctgggtgtga ttatcatccc ggcaattttg ctgctgattg
    10921 gtgtcttctt cctgccagac agcccacgtt ggtttgccgc caaacgccgt tttgttgatg
    10981 ccgaacgcgt gctgctacgc ctgcgtgaca ccagcgcgga agcgaaacgc gaactggatg
    11041 aaatccgtga aagtttgcag gttaaacaga gtggctgggc gctgtttaaa gagaacagca
    11101 acttccgccg cgcggtgttc cttggcgtac tgttgcaggt aatgcagcaa ttcaccggga
    11161 tgaacgtcat catgtattac gcgccgaaaa tcttcgaact ggcgggttat accaacacca
    11221 cagagcaaat gtgggggaca gtgattgtcg gcctgaccaa cgtacttgcc acctttatcg
    11281 caatcggcct tgttgaccgc tggggacgaa aaccaacgct aacgctgggc ttcctggtga
    11341 tggctgctgg catgggcgta ctcggtacaa tgatgcatat cggtatccac tctccgtcgg
    11401 cgcagtattt cgccatcgcc atgctgctga tgtttattgt cggttttgcc atgagtgccg
    11461 gtccgctgat ttgggtactg tgctccgaaa ttcagccgct gaaaggccgc gattttggca
    11521 tcacctgctc cactgccacc aactggattg ccaacatgat cgttggcgca acgttcctga
    11581 ccatgctcaa cacgctgggt aacgccaaca ccttctgggt gtatgcggct ctgaacgtac
    11641 tgtttatcct gctgacattg tggctggtac cggaaaccaa acacgtttcg ctggaacata
    11701 ttgaacgtaa cctgatgaaa ggtcgtaaac tgcgcgaaat cggcgctcac gattaatctc
    11761 ctcaggcttc ctcccgtcgc ggaggaagcc gcctcttgca gtcatctttt cttcgctcta
    11821 tcctctgccg ctatgaaaac atcccgtctc cctatcgcca tccaacaggc cgttatgcgc
    11881 ggcctgcggg aaaaactcgc ccaggccaac ctgaagctag ggcgtaacta cccggagcca
    11941 aaactctctt acacccagcg cggaacctcc gccggaacag cctggctgga aagctatgaa
    12001 atccgcctca atcccgtttt gctgttggaa aacagtgaag cttttattga agaagtggta
    12061 ccgcacgaac tggcacattt gctggtatgg aaacatttcg gccgcgtagc gccacatggc
    12121 aaagagtgga agtggatgat ggaaagcgtg ctgggtgttc ccgcccgtcg tacgcatcag
    12181 ttcgaactgc aatccgtgcg tcgcaacacc ttcccctacc gctgcaagtg ccaggagcat
    12241 cagcttaccg tacgccgcca taatcgcgta gttcgtggcg aggccgtcta tcgctgtgtt
    12301 cactgcggtg aacagctggt tgcgaaataa ccatctgaac tatcaggaac tttcctgatc
    12361 tggctgattg cataccaaaa caactttcgc tacgttgctg gctcgtttta acacggagta
    12421 agtgatgtac cgttatttgt ctattgctgc ggtggtactg agcgcagcat tttccggccc
    12481 ggcgttggcc gaaggtatca atagtttttc tcaggcgaaa gccgcggcgg taaaagtcca
    12541 cgctgacgcg cccggtacgt tttattgcgg atgtaaaatt aactggcagg gcaaaaaagg
    12601 cgttgttgat ctgcaatcgt gcggctatca ggtgcgcaaa aatgaaaacc gagccagccg
    12661 cgtagagtgg gaacacgtcg ttcccgcctg gcagttcggt caccagcgcc agtgctggca
    12721 ggacggtgga cgtaaaaact gcgctaaaga tccggtctat cgcaagatgg aaagcgatat
    12781 gtataacctg cagccgtcag tcggtgaggt gaatggcgat cgcggcaact ttatgtacag
    12841 ccagtggaat ggcggcgaag gccagtacgg tcaatgcgcc atgaaggtcg atttcaaaga
    12901 aaaagctgcc gaaccaccag cgcgtgcacg cggtgccatt gcgcgcacct acttctatat
    12961 gcgcgaccaa tacaacctga cactctctcg ccagcaaacg cagctgttca acgcatggaa
    13021 caagatgtat ccggttaccg actgggagtg cgagcgcgat gaacgcatcg cgaaggtgca
    13081 aggcaatcat aacccgtatg tgcaacgcgc ttgccaggcg cgaaagagct aacctacact
    13141 agcgggattc tttttgttaa cccctacccc acgcgtacaa ccgcgtgggg agacgacgcg
    13201 gatttttaac tatgcgtatc ccccgcattt atcatcctga accactgacc agccattctc
    13261 acatcgcgct ttgcgaagat gccgccaacc atatcgggcg cgtactgcgc atggggccgg
    13321 ggcaggcgtt gcaattgttt gacggtagca accaggtctt tgacgccgaa attaccagcg
    13381 ccagcaaaaa aagcgtggaa gtgaaggtgc tggaaggcaa gatcgacgat cgcgaatctc
    13441 cgctgcatat tcacctcggt caggtgatgt cgcgtggtga aaaaatggaa tttacgatcc
    13501 agaaatcgat cgaactcggt gtaagcctca ttacgccact tttttctgag cgctgcggcg
    13561 ttaaactgga tagtgaacgt ctgaacaaga agctccagca gtggcagaag attgcaattg
    13621 ctgcctgtga gcagtgtggt cgtaaccggg tgccggaaat ccgtccagcg atggatctgg
    13681 aagcctggtg tgcagagcag gatgaaggac tgaaactgaa tcttcacccg cgcgccagta
    13741 acagcatcaa tacgttgccg ttaccggttg aacgcgtccg cctgctgatt ggcccggaag
    13801 gcggtttatc ggcagatgaa attgccatga ctgcccgcta tcaatttact gatatcctgt
    13861 tgggacctcg cgttttgcgt acagagacaa ctgcgctcac cgccattacc gcgctacaag
    13921 tacgatttgg cgatttgggc taacggagaa gaataatgat caagctcggc atcgtgatgg
    13981 accccatcgc aaacatcaac atcaagaaag attccagttt cgctatgttg ctggaagcac
    14041 agcgtcgtgg ttacgaactt cactatatgg agatgggcga tctgtatctg atcaatggcg
    14101 aagcccgcgc ccatacccgc acgctgaacg tgaagcagaa ctacgaagag tggttttcgt
    14161 tcgtcggtga acaggatctg ccgctggccg atctcgatgt gatcctgatg cgtaaagacc
    14221 cgccgtttga taccgagttt atctacgcga cctatattct ggaacgtgcc gaagagaaag
    14281 ggacgctgat cgttaacaag ccgcagagcc tgcgcgactg taacgagaaa ctgtttaccg
    14341 cctggttctc tgacttaacg ccagaaacgc tggttacgcg caataaagcg cagctaaaag
    14401 cgttctggga gaaacacagc gacatcattc ttaagccgct ggacggtatg ggcggcgcgt
    14461 cgattttccg cgtgaaagaa ggcgatccaa acctcggcgt gattgccgaa accctgactg
    14521 agaatggcac tcgctactgc atggcacaaa attacctgcc agccattaaa gatggcgaca
    14581 aacgcgtgct ggtggtggat ggcgagccgg taccgtactg cctggcgcgt attccgcagg
    14641 ggggcgaaac ccgtggcaat ctggctgccg gtggtcgcgg tgaacctcgt ccgctgacgg
    14701 aaagtgactg gaaaatcgcc cgtcagatcg ggccgacgct gaaagaaaaa gggctgattt
    14761 ttgttggtct ggatatcatc ggcgaccgtc tgactgaaat taacgtcacc agcccaacct
    14821 gtattcgtga gattgaagca gagtttccgg tgtcgatcac cggaatgtta atggatgcca
    14881 tcgaagcacg tttacagcag cagtaaccca ccttagcgag aaggatctcg ttgagactct
    14941 gagtgacagc gcccttcttt ccacgcatac tgggcgctgt tgcttttttg aaccaggaaa
    15001 cagaacctct gacaatgaat ttacagcatc actttcttat tgccatgcct gctctccagg
    15061 atccgatttt ccgtcgttcc gtggtctaca tttgcgaaca taataccaat ggtgcaatgg
    15121 ggatcatcgt caacaagccg ctggaaaatc tcaaaattga agggattctg gaaaagctga
    15181 agatcacgcc ggagccgcgt gatgaatcaa tccgtctgga taaaccggtt atgctcggcg
    15241 gtccgctggc tgaagatcgc gggtttattt tgcatactcc gccctccaat tttgcttcca
    15301 gcattcgcat ttcagacaac acagtgatga ccacttcccg cgatgtgctg gaaacgctcg
    15361 gcaccgataa acaaccgtct gacgtattgg tggctctagg ttatgcctcc tgggaaaaag
    15421 gtcaactgga acaagaaatt ctcgataacg cgtggctaac ggccccggcg gatctgaata
    15481 ttctgttcaa aacgccgatt gccgaccgct ggcgcgaggc ggcaaaactg attggtgtgg
    15541 atattctcac catgcctggt gtggcagggc acgcctgatg agtggaacct tactcgcctt
    15601 cgacttcggc accaaaagca ttggcgtagc ggtcggccaa cgcattaccg gcactgctcg
    15661 ccctttgcct gcaattaaag cacaggacgg tacgccggac tggaacctca tcgaacgttt
    15721 actgaaagag tggcagccgg acgaaatcat cgtcggtttg ccgctgaata tggacggcac
    15781 cgagcaacca ttgactgcca gagcgcgtaa atttgccaac cgtattcatg gtcgtttcgg
    15841 tgttgaagta aagctccatg acgaacgtct tagcaccgtg gaagcccgtt ccggtctgtt
    15901 tgaacagggc ggctatcggg cgctcaacaa aggcaaagtc gactctgcct ctgcggttat
    15961 tattctcgaa agctatttcg agcagggata ttaaggcgat ttaaaagcct ggcggatagt
    16021 cgttaccgcc atcttgccgg atgcggcgtg aacgcctatc cggcctacat gtggcagcga
    16081 ttgtaggtct gataagacgc gcaaacgtct catcagacgt ttattgcctt tgtaggcccg
    16141 ataagcttgc acatcgggca tggcaacgtc acaaacgccc ttcccccact cgctgctgaa
    16201 aactctgctg aaacgttatc atccccacct gctgcccggt ttgaataaca tgcggtaact
    16261 ggtgggtttt cccttcgcga atcaaattcc ccaccgcggg agtgttaatc aataattcaa
    16321 acagcgccac gcgtccttcc tgtttatcca cttccagctt ttgtgacaac acggcccgta
    16381 aactacctgc cagttgatta cgtacgggat ctttttcctg cgccggaaat gaatccacca
    16441 gtcgctcaac tgcctgcgcg gcaccacgcg tatgtaatgt tgccagcacc aaatgcccgg
    16501 tttctgccgc cgtcagtgcc agacggattg tctcgctgtc acgcagctct ccgagcaaaa
    16561 tcacatcagg atcttcccgc aatgcggccc gcaatcccga tgcgaacgtc atacagtgca
    16621 aaccaatttc ccgcttctgg atcaaacatc gctggctggc atagatatat tcaacaggat
    16681 cttccagcgt cagaatatgc gcatcggcat gttgattgag atagccaacc atcgccgcca
    16741 gcgtggtaga tttgctgctc cccgtcgccc ccgtcaccag aatcaggcca ttctcactct
    16801 tgagtaattc cggcaatacc gttggtgcgc caagctgttc gagctgcggg cagtgcgaag
    16861 gtaacagccg taacgccagc gaaatgccat gccgttgcgc aaatgcgctg ccacgcaatc
    16921 gctggttttc cgccagcgac acggcaaaat ccagctggcc attttccagc aatattgccc
    16981 gctgatcgtc atccagccac tcccgcagta gctcttcgac gtccggcgcc tcaaacggcg
    17041 cagcttccat tagcccgcga atgcgccatc gtgcgggcca ggcgctgcac aggtgtagat
    17101 ccgagacgtt atgctttaca ctaagggcca caatttcttc catattcata ctaagatcct
    17161 cggaaaatga acgatattgc gcataacctg gcacaggtcc gggacaaaat ctcagcggct
    17221 gcaacgcgtt gcggccgttc tccagaagaa attacgctgc ttgcagtcag taaaacaaaa
    17281 cctgcgagcg ccatcgcaga agccattgat gccgggcagc gtcaatttgg tgaaaactac
    17341 gttcaggaag gggtagataa aattcgccac tttcaggaac tgggcgtaac aggattagaa
    17401 tggcatttta ttggcccgtt gcagtctaat aaaagccgcc tggtggcaga gcatttcgac
    17461 tggtgtcata ccatcgaccg tttgcgcatt gctacccgcc tcaacgatca gcgcccggca
    17521 gaacttcccc ctcttaacgt actgattcaa attaatatta gtgatgaaaa cagtaagtcc
    17581 gggattcaac tggcagaact ggacgagctg gcagcggcgg tcgctgaact accgcgttta
    17641 cgtctgcgcg ggctgatggc aatccctgcg cctgagtcag aatatgtaag gcagtttgaa
    17701 gttgcacgcc aaatggctgt agcatttgcc ggactgaaaa cgcgctaccc gcatatcgac
    17761 acgctctctc tgggaatgtc ggacgatatg gaagccgcca ttgcggcagg tagcacgatg
    17821 gttcgtatcg gcactgcaat ttttggtgcg cgtgattact ctaaaaaata aggaattaaa
    17881 ggaacgccat gaatacgttg actttcctgc tttcaacggt cattgagctg tataccatgg
    17941 tgctgttatt acgcatctgg atgcagtggg ctcattgtga tttttacaac cccttctcac
    18001 agtttgtagt gaaggtaacg cagccaatta tcgggccact gcgccgcgtt attccggcaa
    18061 tgggccccat tgacagcgcc tcgctgctgg ttgcctatat tctcagtttt atcaaagcca
    18121 tcgtgctgtt taaagtggtg accttcctgc caatcatctg gattgctggt ttactgattc
    18181 tgctgaaaac catcggcctg ctgattttct gggtcctgct ggtgatggcg attatgagct
    18241 gggtaagcca gggtcgtagt ccgattgaat acgtgctgat tcagctggcc gatccgctgc
    18301 tgcgcccgat tcgccgcctg ctaccggcaa tgggtgggat tgatttctcg ccgatgatcc
    18361 tcgttctgct gctgtatgtc atcaatatgg gtgtcgcaga agtattacag gcaaccggaa
    18421 acatgctgct gccggggctg tggatggcgt tatgagtgcc gtaacagtta atgatgacgg
    18481 tctggtttta cggctctata ttcagccgaa agccagccgt gattctattg tcggtttaca
    18541 tggcgacgaa gttaaagtcg ccattaccgc gccgccggtt gacggccagg gcaacagtca
    18601 tctggtgaag tttctcggta agcaattccg ggttgccaaa agccaggtgg tgattgaaaa
    18661 aggcgaactt ggccgccaca aacaaattaa aatcattaat ccgcaacaaa tcccgccaga
    18721 aatcgcggcg ttaattaatt aggtatccta tgcaaaaagt tgtcctcgca accggcaatg
    18781 ccggtaaagt gcgtgagctg gcgtcgctgc ttagcgactt cggtcttgat atcgtggccc
    18841 aaacagacct cggcgtagat tccgctgaag aaaccggcct gacctttatc gaaaacgcga
    18901 ttctgaaagc gcgctatgcg gcaaaagtga ccggtttacc ggcaattgcc gacgactctg
    18961 gtctggcggt agatgcgctg ggtggcgcgc cgggtattta ctccgcgcgt tattccggtg
    19021 aagacgcgac cgatcaaaag aatctgcaaa aactgttgga aacactgaaa gacgtaccgg
    19081 acgaccaacg tcaggcgcgt ttccactgcg tgctggtata tctgcgtcac gcggaagatc
    19141 cgactccgct ggtatgccac ggcagctggc cgggcgtgat tactcgcgaa ccagcgggca
    19201 ctggtggctt tggttatgat ccaatcttct tcgtaccttc cgaagggaaa accgccgccg
    19261 aactgacccg cgaagaaaag agcgccattt cccaccgtgg tcaggcgttg aaactgctgc
    19321 tggacgcttt acgtaatggt taaattaccg ccgctgagtc tctacattca catcccgtgg
    19381 tgcgtgcaga aatgcccgta ctgcgatttc aactctcacg cgttgaaagg agaagtgccg
    19441 cacgacgatt atgttcagca tctgctttgc gatctggaca atgatgtggc ttacgctcag
    19501 ggccgtgaag taaagacaat ctttattggc ggtggtacgc cgagcctgct ttccggcccg
    19561 gcgatgcaaa cgctgctgga cggcgtgcgt gcgcgtttgc cgctggcagc ggatgcagaa
    19621 attactatgg aagcgaaccc tggaacggta gaagccgatc gctttgtcga ttatcagcgt
    19681 gctggtgtga accgcatctc tattggtgtg cagagtttta gcgaagaaaa gctgaaacga
    19741 cttgggcgta ttcatggccc gcaagaagcg aaacgcgcgg caaagctggc gagcgggctg
    19801 gggctgcgta gctttaacct cgatttgatg catggactac cggatcaatc actggaagag
    19861 gcgcttggcg atctacgcca ggccattgaa ctgaatccgc cgcatctttc ctggtatcaa
    19921 ctgaccatcg aacccaatac gctgtttggt tcgcgaccac cggtgctgcc ggacgatgac
    19981 gcgctgtggg atattttcga acaggggcat cagttattaa ccgcagcggg ttatcagcaa
    20041 tatgaaactt ccgcttacgc caaacccggt tatcagtgcc agcacaatct caactactgg
    20101 cgatttggtg actacatcgg tattggctgc ggcgcgcacg gcaaagtcac cttcccggat
    20161 gggcgcattc tgcgtaccac taaaacgcgt catcctcgtg gttttatgca aggaaggtat
    20221 ctggaaagcc agcgtgatgt cgaagccgca gataagccgt ttgagttctt tatgaatcgc
    20281 ttccgtctgc tggaggccgc gccgcgcgtg gagtttattg cgtataccgg gctttgcgaa
    20341 gatgtgattc gcccacagtt agacgaggcg attgcccagg gttatctcac cgaatgtgcg
    20401 gattactggc agataaccga gcacggtaaa ttattcttaa actcacttct tgaattattc
    20461 ctcgctgagt aatcattaat gcgcctgcgg gcgcattaat cacttctttt ccccccgtta
    20521 attaacttcg gcaaaatata tttcttactg aataaacatc gatgatattt attagatata
    20581 tctagtgaat atttactcat catttattga ttataaacat gtacaacctg gcgcacaaat
    20641 ttaatattta atttctgact ttatttcaag ttgtgataaa catagacaag ttgtcagata
    20701 ggttaaccaa tttgtcgcac aaagcactga taagcacaat tagatatcaa ccaatttcca
    20761 tccttcaaca gggagttttt ttatgaaaat gaggtcattt acccgctcat tagtctgcgc
    20821 atccttgctg gctctggtta gcactggcgt aaacgccgca gaaaaagtga cgttaaagtt
    20881 agcacacaac ctggaaagaa gtcatgttgt acatcagagt tttgaagagc tggcgaaaga
    20941 ggtaaaacag ctgtcagaag gtaatatggt gatccgtatt tatcccagca gccagatggg
    21001 taatgcgcga taaactatgg aattgttgca aaatggcgca ctggatatga ccaaaggttc
    21061 cgccagtgat ctggagtctt ttgataatat ttatgctatc tataatttgc cgttcttatt
    21121 taaagatcag gctcacttca acaaagttgt ttttggtgaa gttggtaaag aaattatgga
    21181 ctcaacaaaa gacaaaggct tttttgcttt atcagcttat gtagcgggta cacgcagttt
    21241 ctatgcgaaa aaaccgatta ctaagccaga agatcttaaa gggctgaaaa ttcgtgttca
    21301 gccaagtccg accaccatca aaatgattga actgatgggt ggttcaccca ctccaatttc
    21361 atttggcgaa gtttatactg cgatgcagca aggtgtagta gatggcgcag aaaataacgt
    21421 gccttcctgg gtgcagactc gacatattga aattgccaaa gttttttctg aagatgaaca
    21481 cgcctcaatt cctgactttt tggttatctc caccaaaaca tggaataaat taaccccaga
    21541 acagaaacaa atcctcgaaa cagcagcgaa gaaatccgaa gcttatcaac aaaaattgtg
    21601 ggaaaaaatt gacgctgaca cacgtgctca ggcgaaagct atggggggtg aaattgtaaa
    21661 agtggataaa gcaccattcc gtgccgcagt tcagcctctg tttgacgact tcaaaaaaga
    21721 tccaaaacaa gctgctttgt tagagaagtt cgacaacgct gctcaataat attccgggcc
    21781 agtctctggc cctgattttc caagatgctt acaatgatat ttaaccgcct gaaacttgcg
    21841 gtagaccgcg tcattgccgc gttttctgtc gccgtcatgc ttgctctggt tgtatgcgta
    21901 gtctggcaag tatttagccg ctacgtactt aaccagccaa gtacattaac cgatgagctg
    21961 gcacgatttt tgatgatctg ggtagggtta ttaggtgc
//
LOCUS       DADMKF010000060        21684 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.061, whole genome shotgun sequence.
ACCESSION   DADMKF010000060 DADMKF010000000
VERSION     DADMKF010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21684
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.061"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(<1..182)
                     /locus_tag="JNC24_002095"
     rRNA            complement(<1..182)
                     /locus_tag="JNC24_002095"
                     /product="16S ribosomal RNA"
     gene            complement(560..1105)
                     /gene="hemG"
                     /locus_tag="JNC24_002096"
     CDS             complement(560..1105)
                     /gene="hemG"
                     /locus_tag="JNC24_002096"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HAY9158839.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQTDVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMIKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAQLTNKPTLK"
     gene            complement(1117..2568)
                     /gene="trkH"
                     /locus_tag="JNC24_002097"
     CDS             complement(1117..2568)
                     /gene="trkH"
                     /locus_tag="JNC24_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HAY9158840.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            complement(2607..3221)
                     /locus_tag="JNC24_002098"
     CDS             complement(2607..3221)
                     /locus_tag="JNC24_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HAY9158841.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITSVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYHQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            complement(3221..4552)
                     /gene="pepQ"
                     /locus_tag="JNC24_002099"
     CDS             complement(3221..4552)
                     /gene="pepQ"
                     /locus_tag="JNC24_002099"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HAY9158842.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHASVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            4742..6931
                     /gene="fadB"
                     /locus_tag="JNC24_002100"
     CDS             4742..6931
                     /gene="fadB"
                     /locus_tag="JNC24_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001490315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HAY9158843.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLVEGAKAVLRQAINGDLDWKAKRQPK
                     LEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDIVVEAVVENPKVKKAVLAETEQKVRQDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVEDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            6941..8104
                     /gene="fadA"
                     /locus_tag="JNC24_002101"
     CDS             6941..8104
                     /gene="fadA"
                     /locus_tag="JNC24_002101"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HAY9158844.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQAGLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSGAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVSGTVTAGTSSALSDGAAAMLVMSESRARELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMEHKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(8294..8995)
                     /gene="fre"
                     /locus_tag="JNC24_002102"
     CDS             complement(8294..8995)
                     /gene="fre"
                     /locus_tag="JNC24_002102"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HAY9158845.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDIPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHP
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(9042..10535)
                     /gene="ubiD"
                     /locus_tag="JNC24_002103"
     CDS             complement(9042..10535)
                     /gene="ubiD"
                     /locus_tag="JNC24_002103"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HAY9158846.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IEQGETAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            10702..11190
                     /gene="rfaH"
                     /locus_tag="JNC24_002104"
     CDS             10702..11190
                     /gene="rfaH"
                     /locus_tag="JNC24_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HAY9158847.1"
                     /translation="MQSWYLLYYKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTINATRGVSHFVRFGASLAIVPSAVIHQLSVYKPK
                     DIVDPATPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            complement(11187..11969)
                     /gene="tatD"
                     /locus_tag="JNC24_002105"
     CDS             complement(11187..11969)
                     /gene="tatD"
                     /locus_tag="JNC24_002105"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HAY9158848.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAADLNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVAHGIYIGITGWVYDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTRFGIAF"
     gene            complement(12011..12787)
                     /gene="tatC"
                     /locus_tag="JNC24_002106"
     CDS             complement(12011..12787)
                     /gene="tatC"
                     /locus_tag="JNC24_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HAY9158849.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            complement(12790..13305)
                     /gene="tatB"
                     /locus_tag="JNC24_002107"
     CDS             complement(12790..13305)
                     /gene="tatB"
                     /locus_tag="JNC24_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatB"
                     /protein_id="HAY9158850.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            complement(13309..13578)
                     /gene="tatA"
                     /locus_tag="JNC24_002108"
     CDS             complement(13309..13578)
                     /gene="tatA"
                     /locus_tag="JNC24_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HAY9158851.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            complement(13657..15297)
                     /gene="ubiB"
                     /locus_tag="JNC24_002109"
     CDS             complement(13657..15297)
                     /gene="ubiB"
                     /locus_tag="JNC24_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HAY9158852.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVATLEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            complement(15294..15899)
                     /gene="ubiJ"
                     /locus_tag="JNC24_002110"
     CDS             complement(15294..15899)
                     /gene="ubiJ"
                     /locus_tag="JNC24_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HAY9158853.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            complement(15913..16668)
                     /gene="ubiE"
                     /locus_tag="JNC24_002111"
     CDS             complement(15913..16668)
                     /gene="ubiE"
                     /locus_tag="JNC24_002111"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HAY9158854.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            complement(16763..18190)
                     /gene="rmuC"
                     /locus_tag="JNC24_002112"
     CDS             complement(16763..18190)
                     /gene="rmuC"
                     /locus_tag="JNC24_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HAY9158855.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQNEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            complement(18331..19092)
                     /gene="udp"
                     /locus_tag="JNC24_002113"
     CDS             complement(18331..19092)
                     /gene="udp"
                     /locus_tag="JNC24_002113"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HAY9158856.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTASSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGIMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESQAVKIVVEAARRLL"
     gene            19354..20166
                     /locus_tag="JNC24_002114"
     CDS             19354..20166
                     /locus_tag="JNC24_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HAY9158857.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTLDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHAASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPILGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGEKRS"
     gene            20728..21624
                     /gene="hcp"
                     /locus_tag="JNC24_002115"
                     /pseudo
     CDS             20728..21624
                     /gene="hcp"
                     /locus_tag="JNC24_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546094.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
BASE COUNT         5319 a         5804 c         5420 g         5141 t
ORIGIN      
        1 ttatgcggta ttagctaccg tttccagtag ttatccccct ccatcaggca gtttcccaga
       61 cattactcac ccgtccgcca ctcgtcagcg aagcagcaag ctgcttcctg ttaccgttcg
      121 acttgcatgt gttaggcctg ccgccagcgt tcaatctgag ccatgatcaa actcttcaat
      181 ttaaaagttt gatgctcaaa gaattaaact tcgtaatgaa ttacgtgtcc actcttgaga
      241 cttggtattc atttttcgtc ttgcgacgtt aagaatccgt atcttcgagt gcccacacag
      301 attgtctgat aaattgttaa agagcagtgc cgcttcgctt tttctcagcg gcgcggggtg
      361 tgcataatac gctttcccgc tacagagtca agcatttatt tttgctttct ctgtcggaat
      421 tctcaggaga accccgctga cccggcggcg tgtttgccgt tgttccgtgt cagtggtggc
      481 gcattatagg gagttattcc ggcctgacaa gaggaaattt aaaataattt tctgaccgcg
      541 cattttttat tctttatgct tatttcagcg tcggtttgtt ggttaactgg gcgatttctc
      601 gggcgaaatt cgccacctgc tcccaatcgg tatagacaac ttctttgcgc gtatccgttt
      661 caccgcctga catcttcata attagcttga tcataaaacg atcgtaccag cgataacgtg
      721 ggtaacgcag cgccccggca atgaccgcgc agcgatcggg acgccattgc gagttcatca
      781 gaaacttccg cgcgtagctg ttggtctgtg gagtacgctt ctccggtttg cgcgccacca
      841 ggtttacgga ataaaaggcg ctcggcatcg aattcagtcg cgtcgcatgt tttttgacaa
      901 attcctggaa cgcagagtgg tagtgaccat agcgaataga agcaccaatg accacacggt
      961 cataattttc ccattgtggt tcttcaatgc ggtgcacatt ggcgacatcc gtctggatcc
     1021 ccagttcttt cagttccgaa gccaggtagg aggcaatctc gcgcgtttgt ccgtcccttg
     1081 ttgagaaaag aattaatgtt ttcacgtatt actccatcat tcacgccaga aagtcggggt
     1141 aaagagcacc agcaatgtaa agacctcgag acgaccaaac agcatgttgg caatcaggat
     1201 ccatttagcc accgggttca tactggtaaa gttatcagca accacgccaa gccctggccc
     1261 caggttattc aatgtcgcaa caactgaagc aaaggcagaa aagtcatcca cgcccgtggc
     1321 gataatcgcc agcatactga caataaacac caatgcatag gcggagaaaa atccccaaac
     1381 ggcttcgagg atacgttccg gcagtgcgcg attccccagc ttaatgctat agacggcgtt
     1441 cggatgcacc agtcgtttca gctcacggtt cccctgctta aacagcagca ggatgcggat
     1501 cactttcagg ccaccgcccg ttgacccggc gcaaccgccg ataaaagctg aacataaaag
     1561 cagtaccggc aaaaagagcg gccagcgggc aatgctgtca gtcgtaaacc cggcggttgt
     1621 cgccatcgat accacctgga aaaacgcctg gttaattgtc atcagcgccg aactgtagac
     1681 attatgaaac cacagtacga gtgtacaaat aaccaccagc gtaaactgta cgccgataaa
     1741 catgcgaaat tccggatcgc gccaataaac cttcagacta cgcccactta acagtgaaaa
     1801 gtgcagaccg tagttacagc cggagatcag caggaagata gcaatgatag tgttaatagt
     1861 cgggctgtcg aaataaccga tactggcatc atgtgtcgag aagccgccaa tagcgatagt
     1921 cgcaaagcta tggccgatgg catcaaaggc atccattccg gcaaaccaca acgccagcgc
     1981 acaggcgacg gtcagcaaga cataaatcaa ccacagggtt ttcgccgttt ccgcaattcg
     2041 cgggcgcatt ttgttatctt tcagcgggcc gggcatttct gcgcgataga gctgcatccc
     2101 acccacgccg aggataggca gtatcgcaac cgctaacacg atgatcccca tcccgccaaa
     2161 ccattgcagc atctggcgat aaaagaggat ggcatgaggg agcgaatcca gccccaccag
     2221 cgtagtggcc ccggtggtgg tcaggccaga gaaagattca aaaaacgcat cggtaatcgt
     2281 gaggttcggg ctttccgaga agataaaagg gagcgcaccg acactaccca gcacggtcca
     2341 gaacagcacc actatcagaa acccctcacg ggatttcagt tcgcccttct ctttacggtt
     2401 cggccaccac agcatagaac caatggcgag ggcgacaaaa aaggtctggg taaaagcgcg
     2461 gcccgctcca tcccggtaga tgagtgctac cagcccaggg ataatcatgg tccctgaaaa
     2521 taagatgacc agtagtccaa cgattcgggt aatggcgcga aaatgcatct ctgccgcttc
     2581 cttagttctg caaaacgaag tggggattat tcttcaatcg ctaacaattg caatgaacca
     2641 cggctaaaat ccgccagctt tgcggaaaat tcagccactt tcgccgccgg aagcgccacc
     2701 cgcagcagaa cgaatgcctg ataatcactg ttgataattt tgccgtcaca ctgccccagc
     2761 aacgcttcaa tgccggttaa ctgatgatat tcacattgca aagtatattc ggttaatggc
     2821 gtcttgcgtt gggtcgttag ctggcgcagc gcctgattca cgccgccgcc atacgctttc
     2881 actaacccac cggtgccaag caatatgccg ccgtagtagc gcactaccac agaggtaatt
     2941 tccccgacgc cgctgcccat tagctgggcg agcatcggtt tacctgccgt tcctgccggc
     3001 tccccgtcgt cagagaaacc cagctgttga gaatcatccg gcgcacccgc cacccacgcc
     3061 acgcaatggt ggcgggcatc ggggtgttct gcccgcaccg attcaacaaa cgctttcgcc
     3121 gcctcaacgc catcggtatg cgccaacatc gttatgaagc ggcttttttt gatctcttca
     3181 acgaccgtga ccggtgccgc aggaattaac cagctttcca tcacgccagt ttcagatccc
     3241 gggtcatgtt ttccacgttg ttttcgtgga tcaccacgtt gtcttcgata cgaatgccgc
     3301 cgaacggttt cagtgcttca attttctgcc agttgaagtg cttgctgaac tgcccttcac
     3361 gccacggtgc cagtagcgat tcaatgaagt agatacccgg ttcgatggtt aacaccatac
     3421 ccggctggag aatacgggtg cagcgcaggt acggatattt tgccggtgcc gcgaggtgtg
     3481 taccgctatc atcctgcata aaaccggcta cgtcatgcac ctgcaggccc agcggatgac
     3541 cgataccatg cggcataaac ggtccggtga gatcgttttc gaccatcgct tcttcactca
     3601 tatcggtgat gatttgatgt ttacgcagca gtttggcgat gcgctgatgg aactggatgt
     3661 ggtaatccac atagctaacg cctgctttca tggtggcgat cagcgccagt tgttcatcat
     3721 ttacgtcttt caccagctgt gcgtagtcgt tgtcgctttt tgccgaccag gtacgagtca
     3781 ggtcagccgc atagccgtta tattcggccc cggcatccag caggaagctg cgcatctctt
     3841 ccggtgcctg atgatccagt ttggtgtaat gcagcaccga agcgtgttcg ttaagcgcca
     3901 caatgttgct gtaaggtacg tcggtatcac gatgaccggt cgcggtcaga taggcgatgt
     3961 tgatatcgaa ctcgctcatg ccagaacgga acgcttcttc tgccgcacga tgaccgttga
     4021 ccgccatttt ctgcgcttca cgcatacagg ccagttcgta ctcggttttg aaggagcggt
     4081 agtaatgcag gtagtcgatc acccctttcg ggttgatgtt gctggcctca atacccagtt
     4141 gcagcgcacg ttccggcacc ggaccgatat aaccgatatt gccgcgcgca gcgggcagca
     4201 ggctaccaat gccatcggct ttcggcagtg cgatcacttc cacatcttca gtccagaagg
     4261 aggtcggcag cggttcgacg ttgtgccagt aatcaaccgg cagatagaac cacagtttcg
     4321 gcttgttcac gccatccacc agcaaccagc agtttggcac ctgagttacc ggcacccacg
     4381 ctttgaattg cgggttcact ttaaacggat agggatgatc gtcgagaaaa acgttgaaca
     4441 gctcgccgga gtgaataagt aatgcatcca gcttgaagcg cgccagcgca tcgcgagtcc
     4501 gttcttgtaa ggtagctata tgatttttat agagcgaggc cagtgattcc attttttacc
     4561 cttctgtttt tttgacctta agtctccgca tcttagcaca tcgttcatcc agagcgtgat
     4621 ttctgccgag cgtgatcaga tcggcatttc tttaatcttt tatttgcata tttttaacac
     4681 aaaatacaca cttcgatgca tctggtacga ccagatcacc ttgcggattc aggagactga
     4741 catgctttac aaaggcgaca ccctgtacct tgactggctg gaagatggca ttgccgaact
     4801 ggtatttgat gccccaggtt cagttaataa actcgacact gcgaccgtcg ccagcctcgg
     4861 cgaggccatc ggcgtgctgg aacagcaatc agatctaaaa gggctgctgc tgcgttcgaa
     4921 caaagcagcc tttatcgtcg gtgctgatat caccgaattt ttgtccctgt tcctcgttcc
     4981 tgaagaacag ttaagtcagt ggctgcactt tgccaatagc gtgtttaatc gcctggaaga
     5041 tctgccggtg ccgaccattg ctgccgtcaa cggctatgcg ctgggtggtg gctgcgaatg
     5101 cgtgctggcg accgattatc gtctggcgac gccggatctg cgcatcggtc tgccggaaac
     5161 caaactgggc atcatgccgg gctttggcgg ttctgtacgt atgccacgta tgctgggcgc
     5221 tgacagtgcg ctggaaatca ttgccgccgg taaagatgtc ggcgcggatc aggcgctgaa
     5281 aatcggtctg gtggatgggg tagtcaaagc agaaaaactg gttgaaggcg caaaggcggt
     5341 tttacgccag gccattaacg gcgacctcga ctggaaagcg aaacgccagc cgaagctgga
     5401 accgctaaaa ctgagcaaga ttgaagccac catgagcttc accatcgcta aaggaatggt
     5461 cgcgcaaacg gcggggaaac attatccggc ccccatcacc gcagtaaaaa ccattgaagc
     5521 tgcggcccgt tttggtcgtg aagaagcctt aaatctggaa aacaaaagtt ttgtcccgct
     5581 ggcgcatacc aacgaagctc gcgcactggt cggcattttc cttaacgatc aatatgtaaa
     5641 aggcaaagcg aagaaactca ccaaagacgt tgaaaccccg aaacaggctg ctgtgctggg
     5701 cgcaggcatc atgggtggcg gtatcgctta ccagtccgcg tggaaaggcg tgccggttgt
     5761 catgaaagat atcaacgaca agtcgttaac cctcggcatg accgaagccg cgaaactgct
     5821 gaacaagcag cttgagcgcg gcaagatcga tggtctgaaa ctggctggcg tgatctccac
     5881 gatccaccca acgctcgact acgccggatt tgaccgcgtg gatattgtgg tagaagcggt
     5941 tgttgaaaac ccgaaagtga aaaaagccgt actggcagaa accgaacaaa aagtacgcca
     6001 ggataccgtg ctggcgtcta acacttcaac cattcctatc agcgaactgg ccaacgcgct
     6061 ggaacgcccg gaaaacttct gcgggatgca cttctttaac ccggtccacc gaatgccgtt
     6121 ggtagaaatt attcgcggcg agaaaagctc cgacgaaacc atcgcgaaag ttgtcgcctg
     6181 ggcgagcaag atgggcaaga cgccgattgt ggttaacgac tgccccggct tctttgttaa
     6241 ccgcgtgctg ttcccgtatt tcgccggttt cagccagctg ctgcgcgacg gcgcggattt
     6301 ccgcaagatc gacaaagtga tggaaaaaca gtttggctgg ccgatgggcc cggcatatct
     6361 gctggacgtt gtgggcattg ataccgcgca tcacgctcag gctgtcatgg cagcaggctt
     6421 cccgcagcgg atgcaaaaag attaccgcga tgccatcgac gcgctgtttg atgccaaccg
     6481 ctttggtcag aagaacggcc tcggtttctg gcgttataaa gaagacagca aaggtaagcc
     6541 gaagaaagaa gaagacgccg ccgttgaaga cctgctggca gaagtgagcc agccgaagcg
     6601 cgatttcagc gaagaagaga ttatcgcccg catgatgatc ccgatggtca acgaagtggt
     6661 gcgctgtctg gaagagggca ttatcgccac tccggcggaa gcggatatgg cgctggtcta
     6721 cggcctgggc ttccctccgt tccacggcgg cgcgttccgc tggctggaca ccctcggtag
     6781 cgcaaaatac ctcgatatgg cacagcaata tcagcacctc ggcccgctgt atgaagtgcc
     6841 ggaaggtctg cgtaataaag cgcgtcataa cgaaccgtac tatcctccgg ttgagccagc
     6901 ccgtccggtt ggcgacctga aaacggctta aggagtcaca atggaacagg ttgtcattgt
     6961 cgatgcaatt cgcaccccga tgggccgttc gaagggcggt gcttttcgta acgtgcgtgc
     7021 agaagatctc tccgctcatt taatgcgtag cctgctggcg cgtaacccgg cgctggaagc
     7081 ggcggccctc gacgatattt actggggttg tgtgcagcag acgctggagc agggttttaa
     7141 tatcgcccgt aacgcggcgc tgctggcaga agtgccgcac tctgtcccgg cggttaccgt
     7201 caatcgcttg tgtggttcat ccatgcaggc actgcatgac gcagcacgaa tgatcatgac
     7261 tggcgatgcg caggcaggtc tggttggcgg cgtggagcat atgggccatg tgccgatgag
     7321 tcacggcgtc gattttcacc ccggcctgag ccgcaatgtc gccaaagcgg cgggcatgat
     7381 gggcttaacg gcagaaatgc tggcgcgtat gcacggtatc agccgtgaaa tgcaggatgc
     7441 ctttgccgcg cggtcacacg cccgcgcctg ggccgccacg cagtcgggcg catttaaaaa
     7501 tgaaatcatc ccgaccggtg gtcacgatgc cgacggcgtc ctgaagcagt ttaattacga
     7561 cgaagtgatt cgcccggaaa ccaccgtgga agccctcgcc acgctgcgtc cggcgtttga
     7621 tccagtaagc ggtacggtaa cggcgggtac atcttctgca ctttctgatg gtgccgcggc
     7681 catgctggtg atgagtgaaa gccgcgcccg tgaattaggt cttaagccgc gcgcccgtgt
     7741 gcgttcgatg gcggtcgttg gttgtgatcc gtcgattatg ggttacggcc cggttcctgc
     7801 ctcgaagctg gcgctgaaaa aagcggggct ttctgccagc gatatcggcg tgtttgagat
     7861 gaacgaagcc tttgccgcgc agatcctgcc gtgcattaaa gatctcgggc taatggagca
     7921 gattgacgag aagatcaacc tcaatggtgg tgcgatcgcg ctgggtcatc cgctgggttg
     7981 ttccggtgcg cgtatcagca ccacgttgct gaatctgatg gaacacaaag acgttcagtt
     8041 tggtctggcg acgatgtgta tcggtctggg tcagggtatt gcgacggtgt ttgagcgggt
     8101 ttaaccactt gccggatgcg gcgtgaacgc cttatccggt ctaccgatcc tgcaccaatg
     8161 taggcctgat aagacgcgcc agcgtcgcat caggcatcgt gcaccgattg ccggatgcga
     8221 catagatcta aatgccactg aataaagttt tagttgccgt tcttcccgcc tgtcaggggc
     8281 gggtttttat atctcagata aatgcaaacg catcgccaaa cagacgatct tcccgcgcat
     8341 tacgctcact gcaaaacagg tcacgagcaa ttttcgccat ctcaaaacgt ccggcaatat
     8401 agatatcatg ctctgccagc gtaccatgat cctgcaagac cgccgttaac acggtgccag
     8461 tacgcccacg ccagcccgct tccggttgtt caaccaccgg caccacttgc agaccaggat
     8521 gcttcagcga aagcgcctca agctcgcaga gatcatacag atgttgctct tcacgcccac
     8581 cccagtaaat ggtgatatcg cgattgggat tacgcgccaa cgccgtcaat aaaatcgagc
     8641 gcgcatagga gaacccggtg ccgcccgcaa tcaaaatcat cggacgctct tcatcatcgc
     8701 gcagccacgc ttctccgtgg ggaatatcga ccacgatttg atgatctttg aggatgcggt
     8761 ccatgactgc tttcgcgtaa aggttgattt cagaagcgcc aatatgcagc tcgataaacc
     8821 ctttttcatc cggcgtcgaa gccattgaaa acggacgttt gtcgcgctca tccatcacta
     8881 ccatcaaata ctgaccagca cgaaaagaaa aggccgcgtc tggtacgatt cggacacggt
     8941 atacagtatc cgtgatagct tctaccgagg tcactttaca gcttaaggtt gtcatgcgct
     9001 ttctctgtcg gatcaaataa atagggcaaa actaacgcgc atcaggcgct tttaccgttg
     9061 ttaaaaatag ccagttcatc ccagatggcg tcaatatgcg cgacgacatc aggatctttt
     9121 ttgatgggac gtccccattc acgctgggtt tcccccggcc atttattcgt ggcatccagc
     9181 cccatttttg aacccagccc ggagacaggc gaggcaaaat ccagataatc aataggcgta
     9241 ttttctacca gaacagtatc ccgcgccggg tccatacggg tggtaatcgc ccaaatcaca
     9301 tcgttccagt cgcgtgcgtt gacgtcatca tcgcaaacga tcacaaattt agtgtacata
     9361 aactggcgta agaacgacca gacgcccatc atgacgcgct tcgcgtgtcc ggcgtactgt
     9421 tttttgattg tcactaccgc cagacgataa gaacagcctt ccggtggcag gtaaaaatcg
     9481 acaatttccg ggaactgttt ttgcagaatc ggcacaaaca cttcgttcag tgcgacaccc
     9541 agcaccgcgg gttcatccgg cggacgacca gtataggtgg agtggtaaat cgcatcttca
     9601 cgctgggtaa tatgcgtcac ggtaaatacc gggaaactat cgacttcgtt atagtaaccg
     9661 gtgtggtcgc catacggccc ttccggcgca gtttcgcctt gttcgatata cccttccagc
     9721 acaatctccg cactggcggg cacttcaagg tcattggaga tacacttcac cacttcggtc
     9781 ttggtaccgc gcagcaatcc ggcaaacgca tactctgaaa gtgtatccgg aacgggggtg
     9841 actgcaccga gaatcgtggc gggatcggca cccagcgcca cagaaaccgg gaaacgttcg
     9901 cccggatgcg ccgcacacca ctcctgataa tccagcgcgc cgccgcgatg cgacagccag
     9961 cgcataatca gtttgttttt accaatcagc tgctggcgat aaatgcccag attctgccgc
    10021 tctttatgcg ggccgcgcgt tacggtcagc ccccaggtaa tcagcggcgc ggcatcttcc
    10081 ggccagcagg tcataatggg aatgcgattg agatcgacgt catcgccaga gacgattttt
    10141 tgttggcagg gcgcaccacg cagccgcttt gtcggcatgt tcaatacttg cttaaactgc
    10201 ggcagtttat caaacaggtc gcggaaacct tttggcggct cgggctcttt cagaaacgcc
    10261 aataatttac caacttcacg cagcgccgaa acatcttcct gccccatgcc catcgccacg
    10321 cgctttggcg taccgaacag gttgcacagc accggcattg agtagccttt agggttttcg
    10381 aacaacagcg caggcccacc ggcacgcaaa gtgcggtcag caatttcagt gatttccaga
    10441 tgcggatcca ccgggagcgt gatacgtttt agctcaccct gctgttcaag cagcgtcaag
    10501 aagtcgcgta aatcgttata tttcatggcg tccattgtag cctcttaatc tacgcccatt
    10561 atacggcgtt catctttgcg atgctgtaaa tttgttaaat tagcgtgaac tctgacggta
    10621 taacgcaaac cggggaatat aattaactta gcgtaaagct tttgctatcc ttgcgccccg
    10681 attaaacgga taagagtcat tatgcaatct tggtatttac tgtactacaa gcgcgggcaa
    10741 cttcaacgtg cccaggaaca ccttgagaga caggctgtga attgcctggc accgatgatc
    10801 accctggaaa aaatcgtgcg tggaaaacgt actgcagtca gtgagccatt gtttcccaac
    10861 tacctgtttg tcgaatttga tccagaagtg attcatacca cgactatcaa cgcgacccgc
    10921 ggtgtcagcc acttcgtgcg ctttggcgcg tcgctagcga tagtcccatc ggcggtgatt
    10981 catcagctat cggtatataa accgaaagac attgtcgatc cggcaacccc ttatccggga
    11041 gataaggtga ttattaccga aggcgcgttc gaaggctttc aggccatttt caccgaaccc
    11101 gatggtgagg ctcgctccat gctattgctt aatcttatta ataaagagat taagcacagt
    11161 gtgaagaaca ccgagttccg caaactctaa aacgcaatcc caaaccgtgt tttgacatta
    11221 gcatccgtgg tggcagccag ccatgcggca tcttctccac gccagtgcgc aatacgttgc
    11281 aaaatatggg gcagatgggc tggctcgttg cgccgggatg atggctttgg cgtgagatcg
    11341 cgagggagca gatacggcgc atcagtttcg atcagtaatt tttccgccgg aatcaacggc
    11401 aaaagttccc gcagctccag tccgcgtcgt tcatcgtaaa cccaaccggt aatgccgata
    11461 taaattccat gcgccacgca cgcctgcatc tcttcgcgtg tgccggtaaa gcaatgaaga
    11521 accgcaccag gcagtttatc cagccacggc tccagcaatg tcataaaccg ctcgtgggca
    11581 tcgcgacagt gcataaatac cggcatgttt aaatctgcgg caatgcgtag ctgggcaaca
    11641 aaagcgcgtt cctgctcttc cggcgtcgaa aagttgcggt taaagtcgag accacattca
    11701 ccaatcgcca ccacttctgg ctgcgcggcc agctcaataa tcgcttcttc agtcgcagct
    11761 tgccactggc tgctgtcgtg aggatgtacg cccgccgttg accaacagga cgaatactga
    11821 cgcgccagct tttgcgcctg ctggctttca cgcaggttag tgccggtgat gagtagccca
    11881 ttaactcccg cgtcaaaagc gcgcgctaca acatcatcac ggtctttcgc aaattgcgaa
    11941 ctggtcaaat taacgccgat atcaaacatc ctgtactcca tatgacaacc gccctgacgg
    12001 gcggttgaat ttattcttca gttttttcgc tttctgcttc agcgtcgttt tcctcttccc
    12061 gatttcgccc tttaccaacg taaaagcgtg agaagaagac accgatttca aacagacagt
    12121 acatcgggat cgccaatagc gtttgcgaga agacatccgg cggcgtcagc aacatcccga
    12181 caacgaatgc accaaccagc acatacgggc gttttttgcg taagtcttct ggcgaggtaa
    12241 tccccatcca gcacagcagc acaattgcca ccggcacttc aaaggagaca ccaaacgcca
    12301 taaacagcgc cataacgaag cttaaatagc tggcgatgtc ggtggatacc tgcacccctt
    12361 ccggcgcggt attggcaagg aagccaaatg ccagcggaaa gaccacaaag taggcgaatg
    12421 ccatgccgat ataaaacagc agagagctgg aaaccagcag cggcaccacc aggcgacgtt
    12481 catgcttata cagcgctggg gcgataaatg cccacacctg atagagaatc accggcgctg
    12541 acagaatcag cgacaccata aaggtcagct tgatcggcgt aaagaacggc gaggccacgt
    12601 cggtggcgat catcgttgaa ccttgcggca actgcttgat caatggcgcg gataccaggt
    12661 gatagatgtc attggcgaaa tagaccagac acaggaatat cacgatcacc gcgataatgc
    12721 agttcagcag acgcttacgc agctcaatta gatgcgtgat aagcggttga gtatcttcta
    12781 cagacatgtt tacggtttat cactcgacga aggggaaggt gcagcggttt tcggttcagc
    12841 gtccgcagca ggttttacca ccggctctgg cgtggtttct ggcttctgtt ccggcgaact
    12901 ggcctgcgtt tgtgcagcgg caggcgttac gccctcatgc gcagcttcat tatctttcac
    12961 caccgggtta tggatggtgt gcgcttcatc gctcgccttt tcaggatcgt ttgcaacgta
    13021 ggaacgcttc atcgactccg cggcctggcg tagttcatcc atcgacgctt tcagttcggg
    13081 cgtcaggtta gtgaggctcg ccttttcaac ctttttcaga ctgtcctgaa actcctggag
    13141 ttttaactcc tgggtcagtt cgttctgcac cgttgtcgcc agtgaacgca acgcgcgaat
    13201 ccagcccgct accgttttta ccgccacagg cagtcgttgc ggccccagaa cgacgaggcc
    13261 gatgatgaac accaatagca gttcgctaaa accgatatca aacacggatt acacctgctc
    13321 tttatcgtgg cgcttcgcgt cttctgtttt agcctgttcc tgattcgtat ccgcctgctt
    13381 atcggcgata gttttcgcag taaaatcagc atcctgactg gttttatcct gctttggttc
    13441 atcatcgctc attgcttttt taaagccttt gatcgacgca ccaagatcgg aaccgatgga
    13501 gccgagcttt ttggtgccaa aaagcagtac aacgatgacg gcaataatca ataactgcca
    13561 aatactgata ccacccatac atgttcctct gtggtagatg atgattaaac aaggccgcat
    13621 tatacgttac acgacccgcc ttgagcgatg aaaaaatcag cgtgttttgc gccaaccgac
    13681 aaaccaggcg atcagaccac ctgccattaa ccagccgggc atcagccccc attcaggtcg
    13741 gctgaccaac aagaatgtgc cacttaatac taacgtagcg ccaattccga gaaaataacg
    13801 cgattgtccc tgacgtacat gatttgactg aagctcgcgg gcaatcttat caacactgtg
    13861 ctgtaaatac ttgccctggc gcaaactgtc gtaaaccaat tcaggcagtt ctggcatttt
    13921 ttcgacccag aacggcgctt tttctttaaa tgctctcacc agggcaggaa taccgacctg
    13981 atctttaatc cacgactcca ggaaaggctt cgctgttttc cataaatcga gctgcggata
    14041 aagctggcgt cctacccctt cgacatagag cagggttttc tggagtaaca ccagttgcgg
    14101 ctgcacttcc atattgaagc gacgcgccgt attaaacaga tttaacagta catgtccaaa
    14161 cgaaatttcg gccagcggtt tctcaaagat aggttcacag accgtacgaa tggcaaattc
    14221 gaactcttca acgttggtat ctggtggcac ccagccagaa tcgacgtgta gctctgccac
    14281 tttgcgatag tcgcgattaa agaaggcgat aaagttttct gccagatagc gtttatcttc
    14341 tttgtttagc gagccaacaa tcccgcaatc aatgccgata tatttcgggt tttccgggtg
    14401 ttcatagctt acgaaaatgt tgccagggtg catatcggca tggaaaaagc tgtcgcgaaa
    14461 gacctgagtg aagaacacct gcacgccacg ttccgccagc aatttcatgt tggtgccatt
    14521 tttctccagc gtcgcaacat cagacaccgg aatgccgtaa atgcgctcca tcaccatcat
    14581 cccttcacta caatagtcag ggtaaacttc cgggatgtag agcatcgggc tgtcttcaaa
    14641 atttcgccga agctgaatgg cgttggcaga ttcccgcagc aaattcagtt catcaatcaa
    14701 ggtcttttcg tactcgcgca ccacttcggt tgggcgcaga cggcgaccat ccggcagcaa
    14761 acgcggcacc cagcgagcca gacggtagat aagtttcaga tccgctttaa taaccggcaa
    14821 aatatccggg cggatgactt taatcaccac ctctttacca ttcgatttca atcgcgcggt
    14881 atgaacctgg gcgatagaag cagaagccag tggcttgatt tcaaaatcgt caaaccacgc
    14941 ttctaccggc aagccgccca ttgcagcttc aatctgctgc ttcgccagct tgccatcaaa
    15001 cggagcaact ttgtcctgca ataacgccag ctgatcggca atatgcggtg gaaaaagatc
    15061 gcggcgggtt gataacattt gcccgaactt gatccaaacc ggccccagtt cttgcagggc
    15121 cagtcgtagt cgctcaccta aaagtttgtc tttatgccga tttggcatcc agaataatga
    15181 gtatcgccat agccgtagcg gcagggtgat acgcattttg gggatcagtt catcaagtcc
    15241 gtagcttaaa aaagtgcgaa tgatgaaata taggcgccgt acttcacctg gcgtcattta
    15301 gcctccagtt tttccagccg tttggtcagg gcatcaacag cacgctcgac ggcagccgtc
    15361 tcttccgcaa accaggccac ttcaagcgga ccgggtgcca tacgccactc ttcagtaatg
    15421 gcttccgcca cataacgttg ctggcgctta atgccgtgat gcaggaactt tgcgcctccg
    15481 cgcatggctt tgctgattcc ttcagcggcg atatcaccgg tataaggggc cagcagttcc
    15541 gcagggtcga actctgccag atctgccagc gcaacgaagt tttgcaccac ctgaatatcg
    15601 ccctgcactt ccagctcacc actgcgaatc agtgcggtaa gctgctggcg atcgcgaagt
    15661 ttcggcaaca cactggcgta ggcgataacg gtgcagtcag catcgcctgc ccattcgccc
    15721 agtacatcaa cctggcgttc gctgaacacc agaatcaatg acgtcgaaaa gccttttacc
    15781 tccacgcgca atactttacc cagcagacgc gagcgggctg ttttcagcgc gggtgagcga
    15841 tacaggaagg tgttgagcag actttcaatt cctgccgtca ctaaaggttt aaaaggcatt
    15901 tccggtctcc tgtcagaact tataaccacg atgcagcgcc acaacccctg ccgtcagatt
    15961 gtagtagtcg acactttcga atccggcatc ctgcatcatg gctttcaggg tatcctgatc
    16021 gggatgcata cggatggatt ctgccagata acggtagctg tcggcgtcgt tcgcgaccag
    16081 tgagccaata cgcggcagca catggaagga gtatgcatca taggctttgc tcagcggctc
    16141 gataattggc ttcgagaact caagcaccag caggcggccg ccgggtttca gcacgcgata
    16201 cattgaacgc agtgctttat ctttgtcggt gacgttacgc agaccaaacg aaatggtgat
    16261 gcaatcaaag gtgttatccg ggaacggcag cgcctcagcg ttcgcctgaa catactcaac
    16321 gttgccaatc acaccgatat tacgcagctt ctcgcgtccc attttgagca tggattcatt
    16381 gatatcagca aggaccactt tgccagtttc tccgaccagg cgggagaatt tcgctgtcag
    16441 gtcgccggtg ccaccagcca gatccagcac ggtctgccca cggcgtacgc cgctgcaatc
    16501 aatcgtgaat cgcttccaca aacgatgaat accaaatgac atcaaatcat tcatgacatc
    16561 gtattttgat gccacggaat ggaaaacgtg ggcgaccata tccgcttttt gttccttcgc
    16621 gacggtctga aaaccaaagt gcgtcgtttc ttgtgactta tccaccatct caatgcctgc
    16681 tcatcaaaaa attgttccag aagtgtaaca gattgggcgt cgatgcccta gatttctacc
    16741 cggcttaact acccctaatg ggctagcgcg actgctgatt atattcatca tcgcgttgat
    16801 aagcttcatc attcggctgc tccggaaccg accgaagtcg atactcttca ttctggctca
    16861 ccgcctgttc agccaaatcc ggattaatct cgcgtttaat ttctactcct aaaccgcgaa
    16921 acgcttctgc ctgcgccagc acatttccgc gacctgaaga gagttttttc attgcctggc
    16981 ggtagttatc ctgcgctttg tcgagacttt gaccaatcgc ggacatgtca tcgatgaaca
    17041 aacgcatctt gtcgtacagc ttgctggcac gatcggcgat ttgctgggcg ttgcggcttt
    17101 gatgctcata acgccacagg ttggcaatag tacgcaacgc caccagcagc gtagtcgggc
    17161 taaccagcat gatattgttt ttcaacgctt cggtgatcag ctccggctgg cggtcaagcg
    17221 ccagtaaaaa agcgggttca acgggaataa acatcagcac gtaatccaga gttcgcagcc
    17281 ccggcagctg ttgataatct ttgcgtccca gcaaacggat atggttacgc accgacgcga
    17341 tatgttcctg tagcgcgctt tcgcgggtgt agtcgtcttc ggcgttaaaa tagcgttcat
    17401 aggcgaccag cgtcattttg gcgtcgatca ccacatcttt tccctgcggc agccgcacga
    17461 tgacatccgg ctgcatccgc gagcgggcgt cattttcgat gctgacctgg gtttcatatt
    17521 catacccttc acgcagaccg gaagcctcca gcacccgcgt caatactacc tcgccccagt
    17581 tgccctgggt tttattgtcg cctttcagcg cgcgcgtcag gttgatcgct tcctgggcca
    17641 tttgcgcgtt gagttgctgg agattgcgaa tttcgtgggt cagggtatgg cgttcttgtg
    17701 cttctttacc gaagctgtcc tgaacctgac ggcggaaacc gtccagttgt tcacgtagcg
    17761 gcgacaacag gctgttcaga ctctgacggt tttgctcatc aacccggcga ttgctgtgct
    17821 caaaaatacg gttggcgagg ttttcaaact gctcactgag gcgctgctcg ctgttaatca
    17881 tctggcgaat tttatcgtca gcatgttgct gtgcggcttc catccgcgtg gttacttcac
    17941 gcagatcggc ctccagagag gtgttaatac tttgcaggct gcgcacttcg ttattgagta
    18001 actcgcactc tgcacgccag tgctcgcttt gggtaatttg ttgttttgcc gcgcttaact
    18061 ccgcgaccat ctcttcacgt tcagctaatt gctcggcttt ttgctgcgca tgttgataac
    18121 tggcaaacag ccagccaatt gccacaccca ccaacgcaat aactgcgtaa accatgattg
    18181 agaaatccac aacgcctcct gacgcaacaa cttacccgca caaaatagaa ttgtgctgta
    18241 caaacgtcca gttggaaagg catttcctgc gaggcgctac gcaaaaatac aaaaggccga
    18301 acgcgtcggc cttcagacag gagaagagaa ttacagcaga cgacgcgccg cttccaccac
    18361 gattttcacc gcttggcttt cagtttgttt catcgtctca gcattcggga tctcttgctg
    18421 ggtgcggtta acgataacac ccgctaccat accggcacgc aggccctgac ttgcacacat
    18481 ggtcagcagg gttgcagatt ccatttcata gttcattatg cccatcgcct gccactcttc
    18541 catagaacct ttaaagtgac gaactacgcg accagagtaa gtatcgtaac gttcctgacc
    18601 tgggtagaag gtatcagaag aagctgtcac gccaacgtga gttgtcgcgc caatggattt
    18661 cgcagcttca accagcgcag tcgtacattc gaaatcagcg acagccggga attccagcgg
    18721 tgcgaagtgc aggctcgcgc catccagacg gacagacgcc gtggtaacca ggacatcacc
    18781 cacattaata tgcggctgaa tagcgcccgt tgtaccgata cgcaggaagg tgcgaatgcc
    18841 cagctgtgcc agctcttcaa cagcaataga ggtagacggg ccgccgatac cggtagagca
    18901 gacgataaca ggtttaccat ccagctctgc acgccaggta gtgaattcgc ggtgagatgc
    18961 cagcttaacc ggcttatcca tcagcgcggc gatcttttcc acacgatccg ggtcgccagg
    19021 gacgatggca agcgtagccc cttgtaaatc gtttttagtg aggccgagat gaaaaacatc
    19081 agacttggac atatacaact cctctgtgaa tcggtttagt cagaagaagc aaaaagacac
    19141 tttaccgaag ggtttaacat tttttcgtga tactcatcac catgacgcaa atgcgttgca
    19201 taaatttacc caccaaaagt gattcaaatc acataaatca ctcttcattc tgcaggactg
    19261 cataaaagat gagccgattc aggcaatgtg atttgttgca tgctgactat agttaacatt
    19321 gcgcaacatt tttataaggg tacggagaat accatggcaa caacacaaca atctggattt
    19381 gcacctgctg catcgcctct cgcttcgacc atcgttcaga ccctggacga cgcgattgtg
    19441 gcgggcttca cctctatccc ttcacaaggg gataacatgc ctgcttacca tgccagacca
    19501 aagcaaagcg atggcccact gccagtggtc attgtagtgc aggaaatttt tggcgtgcat
    19561 gaacatatcc gcgacatttg tcgccgtctg gcgctggagg ggtatctggc tatcgcacct
    19621 gaactttact tccgcgaagg cgatccgaat gattttgccg atatccccac gttgcttagc
    19681 ggtctggtag caaaagtgcc tgactcgcag gtgctggccg atctcgatca tgccgccagt
    19741 tgggcttccc gcaacggcgg cgatgttcat cgtttaatga tcaccggatt ctgctggggt
    19801 ggacgtatca cctggctgta tgccgcgcat aatccacagc taaaagctgc agtggcgtgg
    19861 tacggcaaac tgacgggcga caagtcgctg aattcaccga aacaacctgt tgatatcgca
    19921 accgatctta acgcgccgat tctcggctta tatggcggtc aggataacag cattccgcag
    19981 gagagcgttg aaaccatgcg ccaggcgctg cgggctgcta atgcgaaagc agagattatc
    20041 gtgtacccgg atgccgggca tgcattcaac gctgattatc gcccgagcta tcatgccgaa
    20101 tctgcgaaag atggctggca gcgtatgctg gaatggttta agcagtatgg agagaagcgg
    20161 agttagtttc ctcttagtct atatttgaga tggtgagtaa acgagtgcgt tttgctgcca
    20221 tctctgtccc ccaaaaaata atcagtatga attgattgga gattacgact ccgctatttg
    20281 accttaagaa agtcggcaag tcatagtcat aaatgagtct cttcaatgga agacacctta
    20341 gagatttacc atcaactccc attcacaaac aaacattaat tattatcaac acattcacat
    20401 taattataaa tcatctcaga ccttcctgat aaaaagatat taaagtgaaa cattttgttt
    20461 ttcacatttt agttcatcac ttttaggcgt aaataaaggt aaaaaaaaca acaatactat
    20521 ttctctttca caaaagaatt aacaaaaggt ttttcaagga tgagtgatat tatttacctg
    20581 accataaccg gagaacaaca aggatgtatt tcctctcgtt gtggaacgag cgcatcaata
    20641 ggtaatcgct ggcagattgg ccatgatgat gaaatcttcg cgttctcgct ctctaatagt
    20701 attacgaata caggcaaagg gtcataattg catggcctta gtttctgtaa attgattgac
    20761 aaaagctccc cgttacttat taacgctatt aacaataacg agcagttgtt catggagttt
    20821 gacttctatc ggatcaatag gtttggtcga tgggagaaat attacaacat tcaactccgc
    20881 ggcgcgctct tatcagcaat caatcacctg tttactgaga acaatctgga tacagaagcc
    20941 atcactgtca gttattaata tatactctcc aggcatctga ttgctaatac tgaatttagt
    21001 tattgggcat ttcccgataa ctacaaccgt ctgtttatac cacggccaaa aactaaagct
    21061 ggtaatggtt tcaaaacact taatagtaaa gcggttggca gactgcttgc tgctgtcggc
    21121 atatacaatg ggaatatcga aggattcagg gaaacagcag acaagttggg gggagatgca
    21181 cctgctggtt atgatcaatt tatggataac aaagggttac tcatcgctgg tgcttcaata
    21241 gttgctggag cagttctggg gagaatgaga ctccctgatt tggaatcact tgagcatttc
    21301 ggagccagag gagcagtatc aggaagacca tttaatcccg agcttgccgg tggtccaata
    21361 gaaaacctga cgacagatgg cgtaacaatc aacagggaag gtatcgctat tgttgaaaaa
    21421 catatagcca gatttggtca tgatcctgta aatgaagtga tgatcaatag actgaaagac
    21481 attgagaaag gaaaaatccc cccagaacaa gtagatctta acttctatac tcatgagtgt
    21541 cgggaatatc agcgttactg taatcttggt tgggaaacag gacaacctga tggtgacgca
    21601 ggctatgcac tctggaatca tactggattt gcccctatat ttccagacat ctgttatcac
    21661 ttaacccatt acaagcccgc tgcc
//
LOCUS       DADMKF010000061        21641 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.062, whole genome shotgun sequence.
ACCESSION   DADMKF010000061 DADMKF010000000
VERSION     DADMKF010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21641
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.062"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(<1..1125)
                     /locus_tag="JNC24_002116"
     CDS             complement(<1..1125)
                     /locus_tag="JNC24_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY9158858.1"
                     /translation="MAMSEQPQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYASLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIAD"
     gene            1343..2995
                     /locus_tag="JNC24_002117"
     CDS             1343..2995
                     /locus_tag="JNC24_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HAY9158859.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNPEYFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTREDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGETNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMIGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(3032..3511)
                     /gene="ybaK"
                     /locus_tag="JNC24_002118"
     CDS             complement(3032..3511)
                     /gene="ybaK"
                     /locus_tag="JNC24_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HAY9158860.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMAAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(3715..4509)
                     /locus_tag="JNC24_002119"
     CDS             complement(3715..4509)
                     /locus_tag="JNC24_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9158861.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPTCEALEERINEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQITMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNDITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            4647..4988
                     /locus_tag="JNC24_002120"
     CDS             4647..4988
                     /locus_tag="JNC24_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HAY9158862.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFRLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(5203..7707)
                     /gene="copA"
                     /locus_tag="JNC24_002121"
     CDS             complement(5203..7707)
                     /gene="copA"
                     /locus_tag="JNC24_002121"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HAY9158863.1"
                     /translation="MSQTIDLTLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARVVTADGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGESVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTIVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNEQQVGTKAIEAEITA
                     QASQGATPVLLAIDGKAVALLAVRDPLRSDSVAALQRLHKTGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            7969..8901
                     /gene="glsA"
                     /locus_tag="JNC24_002122"
     CDS             7969..8901
                     /gene="glsA"
                     /locus_tag="JNC24_002122"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HAY9158864.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTCDGNVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENVEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            8904..10196
                     /locus_tag="JNC24_002123"
     CDS             8904..10196
                     /locus_tag="JNC24_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAY9158865.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWVAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASAINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLGSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGSLLLSLIVAMVMKRNKTV"
     gene            10321..10728
                     /locus_tag="JNC24_002124"
     CDS             10321..10728
                     /locus_tag="JNC24_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CueR"
                     /protein_id="HAY9158866.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(10729..11187)
                     /locus_tag="JNC24_002125"
     CDS             complement(10729..11187)
                     /locus_tag="JNC24_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HAY9158867.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLHIRAVSS"
     gene            complement(11184..12101)
                     /locus_tag="JNC24_002126"
     CDS             complement(11184..12101)
                     /locus_tag="JNC24_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="paraslipin"
                     /protein_id="HAY9158868.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGTSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            12247..12924
                     /gene="fetA"
                     /locus_tag="JNC24_002127"
     CDS             12247..12924
                     /gene="fetA"
                     /locus_tag="JNC24_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HAY9158869.1"
                     /translation="MQENSPLLQLQNVGYLAGDAKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRNRQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            12911..13690
                     /gene="fetB"
                     /locus_tag="JNC24_002128"
     CDS             12911..13690
                     /gene="fetB"
                     /locus_tag="JNC24_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HAY9158870.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGSIEFIPMQVIPIAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(13753..14607)
                     /gene="cnoX"
                     /locus_tag="JNC24_002129"
     CDS             complement(13753..14607)
                     /gene="cnoX"
                     /locus_tag="JNC24_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HAY9158871.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNFTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGMVAQIELLKQAADTPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(14668..15477)
                     /gene="ybbO"
                     /locus_tag="JNC24_002130"
     CDS             complement(14668..15477)
                     /gene="ybbO"
                     /locus_tag="JNC24_002130"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HAY9158872.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPDDVERMNSMGFTGVLIDLDSPESVDRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLKRLLPGRVMDKILQG"
     gene            complement(15467..16090)
                     /gene="tesA"
                     /locus_tag="JNC24_002131"
     CDS             complement(15467..16090)
                     /gene="tesA"
                     /locus_tag="JNC24_002131"
                     /EC_number="3.1.1.5"
                     /EC_number="3.1.2.14"
                     /EC_number="3.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
                     /protein_id="HAY9158873.1"
                     /translation="MNFNNVFRWHLPFLFLALLTFRAAAADTLLILGDSLSAGYRMSA
                     SAAWPALLNDKWQSKTSVVNASISGDTSQQGLARLPALLKQHQPRWVLVELGGNDGLR
                     GFQPQQTEQTLRQILQDVKAANAEPLLMQIRLPANYGRRYNEAFSAIYPKLAKEFDVP
                     LLPFFMEEVYLKPQWMQDDGIHPNRDAQPFIADWMAKQLQPLVNHDS"
     gene            16061..16747
                     /locus_tag="JNC24_002132"
     CDS             16061..16747
                     /locus_tag="JNC24_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAY9158874.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            16744..19158
                     /locus_tag="JNC24_002133"
     CDS             16744..19158
                     /locus_tag="JNC24_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY9158875.1"
                     /translation="MIARWFWREWRSPSLLIVWLALSLAVACVLALGNISDRMEKGLS
                     QQSREFMAGDRALRSSREVPQAWLEEAQKRGLKVGKQLTFATMTFAGDTPQLANVKAV
                     DDIYPMYGDLQTNPPGLKPQAGSVLLAPRLMALLNLKTGDTIDVGDATLRIAGEVIQE
                     PDSGFNPFQMAPRLMMNLADVDKTGAVQPGSRVTWRYKFGGNENQLDGYDKWLLPQLK
                     PEQRWYGLEQDEGALGRSMERSQQFLLLSALLTLLLAVAAVAVAMNHYCRSRYDLVAI
                     LKTLGAGRAQLRKLIVGQWLMVLTLSAVTGGAIGLLFENVLMVLLKPVLPAALPPASL
                     WPWLWALGTMTVISLLVGLRPYRLLLATQPLRVLRNDVVANVWPLKFYLPIVSVVVVL
                     LLAGLMGGSMLLWAVLAGAVVLALLCGVLGWMQLNVLRRMTLKSLPLRLAVSRLLRQP
                     WSTLSQLSAFSLSFMLLALLLVLRGDLLDRWQQQLPPESPNYFLINIATEQVAPLKAF
                     LAEHHIVPESFYPVVRARLTAINDKPTEGNEDEALNRELNLTWQNTRPDHNPIVAGNW
                     PPKADEVSMEEGLAKRLNVALGDTVTFMGDTQEFRAKVTSLRKVDWESLRPNFYFIFP
                     EGALDGQPQSWLTSFRWENGNGMLTQLNRQFPTISLLDIGAILKQVGQVLEQVSRALE
                     VMVVLVTACGMLLLLAQVQVGMRQRHQELVVWRTLGAGKKLLRTTLWCEFAMLGFVSG
                     LVAAIGAETALAVLQAKVFDFPWEPDWRLWIVLPCSGALLLSLFGGWLGARLVKGKAL
                     FRQFAG"
     gene            complement(19392..20486)
                     /gene="mnmH"
                     /locus_tag="JNC24_002134"
     CDS             complement(19392..20486)
                     /gene="mnmH"
                     /locus_tag="JNC24_002134"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HAY9158876.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMN
                     NDERAAVGTCYKQQGSDAALALGHKLVAGEIRQQRMDAWRAACLQNPQGILCCARGGQ
                     RSHIVQSWLYAAGIDYPLVEGGYKALRQAAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAAQLDTALTTQLTTDSTDGHLAWLVPLLKEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAEWVKTQ"
     gene            complement(20555..21481)
                     /gene="allS"
                     /locus_tag="JNC24_002135"
                     /pseudo
     CDS             complement(20555..21481)
                     /gene="allS"
                     /locus_tag="JNC24_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
BASE COUNT         5015 a         5684 c         5798 g         5144 t
ORIGIN      
        1 atcggcgata agccccagaa ctgccgctcc cagacctccc atgccaaaag caaaaccgaa
       61 aaagagtcca gaaaccatac cgatacgtcc cggaagtagc tcctgagcgt agaccagaat
      121 ggcagagaat gccgaagcga ggataaatcc aataatcacc gttaaaaccc ccgtccagtg
      181 caggctggcg tagggtaaaa tcagcgtaaa cggcgcaacg ccgaggatag agccccaaat
      241 cacatatttc cgtccaattt tatcccctac aggcccgccg atcaccgtac ctgccgcaac
      301 ggcaaacagg aaggcaaaca gatgaagctg ggcattctgg atagataatc cgaatttttg
      361 catcagataa aaggtgtaat agctgctgat gctcgccata tagaaatatt tcgagaaaat
      421 gaggattaac agaatgctga ctgccagtac cactttgttg cgcggcagtg gattgattat
      481 cgtcgctttg ggttttcctt tattcattcg gtgctgtgcc gagtaccaac ggctaatttg
      541 cgccaacacc acgatcgcca gcagtgccgc aagcacaaac caggcaacgt tgcctttgcc
      601 ataaggcgcg ataatcaccg ccgccagcaa gggtcccagg gaactgccaa agttgccgcc
      661 gacctgaaag atagattgcg ccaggccatg ccgcccgccg gaagccatac gggctacgcg
      721 agaagattcc ggatgaaaga ccgatgaacc ggtaccgacc agcgccgccg ccagcagaac
      781 tgcgccaaaa ctgcccgcca gcgcaagcag caccagacca cttaaggtaa agcacatgcc
      841 aattggcaac gaccacggca tcggatattt atcggtccag tagccgacca ctggttgcag
      901 tagcgaagag gcgagctgga aggtgagggt tatcatgcca atctgcataa atgtcagaga
      961 aaattctgac tgaagcagcg gataaatcgc cagaatcagc gattggatca tgtcgttcag
     1021 cagatgtgag aggctgatag cacctaaaat accaaacgat gttcgggcct tggtcgttga
     1081 cgcagccgcg cccgccacag gctggggttg ttcactcatt gccataggaa agtcactttt
     1141 tcagggttgc gatgtaaaga atgatcttat ttgtgattat tacccgacta acatacctgt
     1201 atgcgtcgtc tgaaggaagt ctcaacgccg aatacagaat ttctaatctg gatgcagatt
     1261 tatcttcacc ggacgcagac ttgtctatga tgtcgcgtca tactattttt caacacgttg
     1321 aaatcaggtc agggagagaa gtatgaaatt attgcagcgg ggcgtggcgt tagcgctgtt
     1381 aaccacattt acactggcga gtgaaactgc tctggcgtat gagcaggata aaacctacaa
     1441 aattacagtt ctgcatacca atgatcatca tgggcatttt tggcgcaatg aatatggcga
     1501 atatggtctg gcggcgcaaa aaacgctggt ggatggtatc cgcaaagagg ttgcggctga
     1561 aggcggtagc gtgctgctac tttccggtgg cgacattaac actggcgtgc ccgagtctga
     1621 cttacaggat gccgaacctg attttcgcgg tatgaatctg gtgggctatg acgcgatggc
     1681 gatcggtaat catgaatttg ataatccgct caccgtatta cgccagcagg aaaagtgggc
     1741 caagttcccg ttgctttccg cgaatatcta ccagaaaagt actggcgagc gcctgtttaa
     1801 accgtgggcg ctgtttaagc gtcaggatct gaaaattgcc gttattgggc tgacaaccga
     1861 tgacacagca aaaattggta acccggaata cttcactgat atcgaatttc gtaagcccgc
     1921 cgatgaagcg aagctggtga ttcaggagct gcaacagaca gaaaagccag acattattat
     1981 cgcggcgacc catatggggc attacgataa tggtgagcac ggctctaacg caccgggcga
     2041 tgtggagatg gcacgcgcgc tgcctgccgg atcgctggcg atgatcgtcg gtggtcactc
     2101 gcaagatccg gtctgcatgg cggcagaaaa caaaaaacag gtcgattacg tgccgggtac
     2161 gccatgcaaa ccagatcaac aaaacggcat ctggattgtg caggcgcatg agtggggcaa
     2221 atacgtggga cgggctgatt ttgagtttcg taatggcgaa atgaaaatgg ttaactacca
     2281 gctgattccg gtgaacctga agaagaaagt gaccagggaa gacgggaaaa gcgagcgcgt
     2341 gctgtacacc cctgaaatcg ctgaaaacca gcaaatgatc tcgctgttat cgccgttcca
     2401 gaacaaaggc aaagcgcagt tggaagtgaa aataggcgaa accaatggtc gtctggaagg
     2461 cgatcgtgac aaagtgcgtt ttgtacagac caatatgggg cggttgattc tggcagccca
     2521 aatggatcgc actggtgccg actttgcggt gatgagtgga ggaggaattc gtgattctat
     2581 cgaagcaggc gatatcagct ataaaaacgt actgaaagtg cagccattcg gcaatgtggt
     2641 ggtgtatgcc gacatgatcg gtaaagaggt gattgattac ctgaccgccg tcgcacaaat
     2701 gaagccagat tcaggtgcct acccgcaatt tgccaacgtt agctttgtgg cgaaagacgg
     2761 caaactcaac gacctgaaaa tcaaaggcga accggtcgat ccggcgaaaa cttaccgtat
     2821 ggcgacatta aacttcaatg ccaccggcgg tgatggctat ccgcgccttg ataacaaacc
     2881 gggctatgtg aataccggct ttattgatgc cgaagtgctg aaagcgtata tccagaaaag
     2941 ctcgccgctg gatgtgagtg tttatgaacc gaaaggtgag gtgagctggc agtaatccga
     3001 aagtgccgga tatttgcatc cggcacaatg cttaatcgcg gcgggcgata tcagcaaatt
     3061 tggcatcgag gatcttcgcc agatcgcctg ccgccagttc gatatccagt ccgcgcttgc
     3121 cgccagaaac ataaatagtg gcaaattctt gtgcgggggc gtcgataatc gtcggcagac
     3181 gttttttctg ccccaatggg ctaatccccc caaccaggta tcccgtcgaa cgctgcgcgg
     3241 ccatcggatc ggccatctca actttcttgg cacccagcgc ttttgctact tttttaagat
     3301 ccagttgacc tgcgaccggc gtaacggcca ctgccagatg tttcatatca ccgttcactg
     3361 ccaccagtag cgttttgtag acttgatccg gattcaaacc taattttttg acgacttcat
     3421 cgccaaaatt ggtttcagcc ggatcgtgct cgtaggtatg gatttgaaac gaaatcttgt
     3481 ttttttcgag taatttaact gcgggtgtca tggtaatttt cctgccttaa acaaaaaaac
     3541 gaagcaagca tacgcctgat ggcggtccaa aaaaaagagt catcttgcct aagagtattg
     3601 gcaggatggt gagattgagc gacaatcgag ttacaccgtc gcttaaagtg acggcataat
     3661 aataaaaaaa tgaaattcct ctttgacggg ccaatagcga tattggccat ttttttagcg
     3721 caacatttgc ggcaaattcc cttctccata caggtgtagt gcaccgaccg cgaccacata
     3781 tcgccccggc ggcatggcgc gtaatttatc ccgccaggcg agatttcgct gatgcatcag
     3841 cacatcgtac agcgactgac tgaacgtatt gggcagcgtt atatcattat tttgcggagg
     3901 tgcattcagc caccagctca tcatttgttg cagcaaccgt gcgttggtat gccagtgggt
     3961 cagcgtatcg tccagcagcg ccagtccttt gtcagggagc tggagcaaca tggtaatctg
     4021 gttttcagcc ccttccagtt caatcacggg tttatgttgt tgcttcgccg cctgcaataa
     4081 ctggtaatcg ataccgtatt ctgcccgcag ccccagtttt tgtgcctgcg tcgcctgaag
     4141 aaccatcgcg atttgccaca gcggttgagt agaaaagagc gacggagaaa tgcccatctc
     4201 ctggctaatg tgctgcagat tttggagttg ctcctcgtta atgcgctctt ccagcgcctc
     4261 gcaagtaggc aaattagcaa aaggcgtatc gctggtggaa acatccgcct cgacgatcag
     4321 cgcatcggcg tttttgagct ttttgagcaa acgggtgggc aggggagcca tatcgtggct
     4381 acccatatga atactgccaa tcagatgaaa atggcgattg ccgggaaggg tgatatcgat
     4441 ggcaggccag gtgtaatgat taccgcgcag cgcggcccaa agcgttttta cccggtacaa
     4501 cagatccata cgacctccct ttgtgaaata tcatgctagc gcgcggtgag ggatggcgca
     4561 aatggagttg gggacttgtt taatgtgttt gtatgattca gtatgttctt gcatcgctat
     4621 tcacagggaa gcaacagtta aaaaccatga aacaggcaac aagaaaaccg acgacacccg
     4681 gagatattct tctctatgaa tatctggaac cgctcgattt gaaaatcaat gagttagcag
     4741 agttgctgca tgttcatcgt aatagcgtca gtgcactgat caataacaat cgtaaactca
     4801 ccactgagat ggcattccgt ctggcgaaag tttttgatac cacagtcgat tttaggctaa
     4861 acctccaggc ggcagttgat ctttgggaag ttgaaaacaa catgcgcacc caggaagaat
     4921 tgggacggat tgaaacagtg gctgaatatt tggcacgccg tgaagagcgt gcaaaaaagg
     4981 tcgcgtaagg ccaaaacatc gcttcatctc gtaggccgga taaggcgttc acgccgcatc
     5041 cggcatccga gcaccattgc ctgatgcgac gcttacccgt cttatcaggc ctacaaacct
     5101 gtgcctgaac cgtaggccgg ataaggcgtt tacgccgcat ccggcagtca tgcgtcgatg
     5161 ccaaatgcgc cgccctaaag cagcgcatcc gcaatgacgt acttattcct tcggtttaaa
     5221 ccgcagcaac cggttggcgt tactcactac ggtaatcgac gagagcgcca ttgccgctcc
     5281 ggcaactacc gggttaagca gtgttcccgt aaacggccac aaaataccgg cggcgaccgg
     5341 aataccgatg ctgttgtaga taaaggcacc gagcaggttc tgcttcatgt tgcgcagcgt
     5401 tgcgcgggaa atcgcgagcg catccgcaac gcccatcagg ctatggcgca tcagggtaat
     5461 cgccgcggtt tcaatggcaa catcactgcc gccacccatc gcaatgccga catccgcctg
     5521 ggccagcgct ggcgcgtcgt taatgccgtc gcccaccatt gccacctgac gtccttcact
     5581 ttgcagacgt ttgatcgctt cggctttacc gtccggcagc actccggcaa tcacttcatc
     5641 aattccagct tctttggcga tcgcattggc ggtggttggg ttatccccgg tcaacatcac
     5701 cagacgatat cccgttttat gcaggcgttg cagcgccgcc acgctatcac tacgcaacgg
     5761 atcgcgtaca gccagcaggg ctactgcttt cccgtcaatc gccagcagca caggcgttgc
     5821 tccttgcgat gcctgagcag taatctccgc ttcgatagct ttggtaccaa cctgttgctc
     5881 atttaacagc gcctgattgc ccagcaataa cgcatgacct tcagcttcac cgctcacgcc
     5941 cagcccgcgc aatgtgcgga aaccgttgac ctgcggcagc tgcatatcac ctgctttatc
     6001 gaggatcgcc cgtgccagcg gatggctgga accttgctcc agtgccgccg ccagacgcaa
     6061 tgcctgcgct tcatcaacat cagcaaacgt tttcactgcg acaacctgcg gcttcccttc
     6121 ggtcagcgtc ccggttttat cgaacacgat ggtatcgagt gtactggcgc gttgcagcgc
     6181 gtcggcgtcc cgcaccagca cgccaaactc agccgcccgc ccgacgccgg aaataatcga
     6241 catcggcgtc gccagcccca gcgcacacgg acaggcgata atcagtaccg tggtggcaat
     6301 caccagggta taaacaattt gcggtgccgg accaaagaag taccagattg ccgcactgac
     6361 cagcgcaatc accaccacca caggtacaaa tacggctgag attttatccg ccagctgacc
     6421 gatttctggc ttgctgctct gggcctggcg caccatgcga atgatccgtg acagcgtggt
     6481 atggctgcca accgcactgg cacgaaacag cacactgccg tcctgtacca ctgtcccggc
     6541 atggacgctt tcgccttcgc ctttttgctg tgggattggt tcgcccgtca gcatcgcttc
     6601 atccagccat gcttcgccct gggtaatctc gccatctacc ggcacgcgat cgccggtcgt
     6661 caggcgcagt aacatccctg gctgaacttc cgccagcggc acgctttttt caccatctgc
     6721 ggtgaccact cgcgccgtcg gtggggttaa atcgagtaat ttctccagcg ccttagaaga
     6781 acgctgacgt gcgcgcgctt ccagcatatg gccgagatta atcagaccga taatcatcgc
     6841 actggcttca tagtaaagat gccgcgcttc catcgggaac cactgcggcc acaggttgac
     6901 gctcatcgaa tagagccacg ccacacccgt tcccagcgcc accagcgtat ccatcgtcgc
     6961 tgcaccgttc agcaggcttt tccatgcact gcggtaaaaa tggccgccgg cgaaaaccat
     7021 cactgccagg gttatcaggc cgataactaa ccacaagctg cggttgtcag cggtgaccat
     7081 catgttatcg ccgatcatcc cccagaccat taccgggata cccacagcca gtgcaacaat
     7141 tgcctgccag cggaagcgct tcatcgtagc tacggcggtt tcttgctggc gctcgcggcg
     7201 tttagcgtca tcttcaatcg cttccgcacc gtagcccgct ttttccaccg cctgcactaa
     7261 atcttgtggg gaggcactgc ccatcaccag cgcagtacgc tccgccaggt ttacccgtgc
     7321 ctgagtgacg cccggtacgc tttgcagcgc attttgtacg cgggtgacac agctggcgca
     7381 gctcatgccg ctcagcagca actgctggct gtcatcgtca tcggcggtcg ctgccggaag
     7441 cgcctcagaa accgctgtca gtgcttccga cgggattgat gactccgcca gcggtttagc
     7501 ctttgggtgg cttacagatg cgtcataacc cgcttgtttg atggtttcga tcagctgttc
     7561 tgcactggca gtcccggtaa cgtgcgcttc agtgatagac acatccgcct gctcaacatc
     7621 cgggcgctgt tcaagacttt ctttcacgcg tttaacgcag tgaccgcagg acaggccgtc
     7681 cagggtcagg tcgatagttt gtgacataaa acactccttt aagacagttt tgactggctg
     7741 tgataaaggt taaaccttcc agcaagggga aggtcaagaa attaataaac caggcgggta
     7801 aaagtccgta aagattaaaa aatcggctcg atttgcatca ggattagaca tttatttctt
     7861 tgttttcctg tagttaagtt gcgggtgcta agttaaatca ggatgcctga aaatcggcac
     7921 cggggtgagg aattacctcc cgcatctata aaaaggagtt aacaaaagat gttagatgca
     7981 aacaaattac agcaggcagt ggatcaggct tacacccaat ttcactcact taacggcgga
     8041 caaaatgccg attacattcc ctttctggcg aatgtaccag gtcaactggc ggcagtggct
     8101 atcgtgacct gcgatggcaa cgtctatagt gcgggtgaca gtgattaccg ctttgcactg
     8161 gaatccatct ctaaagtctg tacgttagcc cttgcgttag aagatgtcgg cccgcaggcg
     8221 gtacaggaca aaattggcgc tgacccgacc ggattgccct ttaactcagt tatcgcctta
     8281 gagttgcatg gcggcaaacc gctgtcgcca ctggtaaatg ctggcgctat tgccaccacc
     8341 agcctgatta acgctgaaaa tgttgaacaa cgctggcagc gaattttaca tatccaacag
     8401 caactggctg gtgagcaggt agcgctctct gacgaagtca accagtcgga acaaacaacc
     8461 aacttccata accgggccat tgcctggctg ctgtactccg ccggatatct ctattgtgat
     8521 gcaatggaag cctgtgacgt gtacacccgt cagtgctcta cgctcatcaa tactgttgaa
     8581 ctggcaacgc ttggcgcgac gctggcggcg ggtggtgtga atccgttgac gcataaacgc
     8641 gttcttcagg ccgacaacgt gccgtacatt ctggccgaaa tgatgatgga agggctgtat
     8701 ggtcgctccg gtgactgggc gtatcgcgtt ggtttaccgg gcaaaagcgg tgtaggtggc
     8761 ggtattctgg cggtcgtccc tggcgtgatg ggaattgccg cgttctcacc accgctggac
     8821 gaagaaggca acagtgttcg cggtcaaaaa atggtggcat cggtcgctaa gcaactcggc
     8881 tataacgtgt ttaagggctg atcatgatga acacggaagg taataacggt aacaaacccc
     8941 tcggtctatg gaacgtcgtt tccatcggta ttggggcaat ggtgggggcg gggatcttcg
     9001 cgctgctggg gcaggctgcg ttgctaatgg aagcctcgac ctgggtcgcc tttgcttttg
     9061 gcggtattgt ggcgatgttt tccggttatg cctatgcgcg tctgggggcg agctatccca
     9121 gcaatggcgg tattatcgac ttttttcgtc gcggattagg caacggcgtc ttttcgctag
     9181 cgctctcgtt gctgtacctg ttgacgctgg cggtgagcat cgccatggtt gcccgtgctt
     9241 ttggcgctta tgccgtgcag tttttgcatg aaggcagcca ggaggagcac cttattttgc
     9301 tctacgcgtt ggggatcatt gcggtgatga cgctttttaa ctccttaagc aaccatgcgg
     9361 tagggcggct ggaagtgatc ctcgtcggca ttaaaatgat gatcctgtta ttgctgatta
     9421 ttgccggtgt ctggtcgctg caaccggcgc atatttccgt ctctgcgccc cccagctccg
     9481 gtgcgttctt ctcctgtatt gggataacct tccttgccta tgcgggcttt ggcatgatgg
     9541 cgaacgcggc ggataaagtg aaagatccgc aggtcattat gccacgggcg tttctggtgg
     9601 cgattggcgt taccacgttg ctttatatct cgctggcgct ggttttgctt agcgatgtat
     9661 cggcattaga gttagaaaaa tatgctgata ccgccgtagc gcaggctgct tctccgctgc
     9721 tcgggcatgt gggttatgtg atcgtcgtca tcggcgcttt actggcgacg gcttcagcca
     9781 ttaacgcgaa cctgttcgcc gtgtttaaca tcatggacaa catgggcagc gaacgcgaac
     9841 tgccgaagct aatgaataaa tccctgtggc ggcagagtac ctggggcaac attattgtcg
     9901 tggtgttgat tatgctgatg acggcggcgc taaatttagg ctcactcgcc agtgttgcca
     9961 gcgccacctt tttgatttgc tacctggcgg tgtttgtggt ggcgatccgc ctgcgtcatg
    10021 atattcacgc ctcgttgccg attcttatcg ttggtacgtt ggtgatgttg ttggtgatcg
    10081 ttggctttat ctacagtctg tggtcccagg gtagccgtgc gttgatatgg attattggct
    10141 cactcttact cagccttatt gtggcaatgg tcatgaagcg caataaaacc gtataacatc
    10201 tctctgtgcg cagtacttcc tgtattattg tggtggcggt cgatattcgc actggcaaaa
    10261 aaacgtgctt gaatatctgt tgaaaccctt taacaaagca caggaggcgt tgcgcgaacg
    10321 atgaacatca gcgatgtagc aaaaattacc ggcctgacca gcaaagccat tcgcttctat
    10381 gaagagaagg ggctggtgac gccgccgatg cgcagcgaaa acggttatcg cacctacacg
    10441 cagcagcatc tcaacgaact gaccttactg cgccaggcac ggcaggtggg ctttaacctg
    10501 gaagagagcg gcgagctggt gaatctgttt aacgacccgc agcggcacag cgccgacgtc
    10561 aaacggcgca cgctggagaa ggtggcggag atcgaacgac acattgagga gctgcaatcc
    10621 atgcgcgacc agctgctggc actggcgaat gcctgccctg gcgatgacag cgccgactgc
    10681 ccgattatcg aaaatctctc cggctgctgt catcatcggg cagggtgatt aagacgagac
    10741 ggctcggata tgtagggtta tcccttctat cgcaatgact tcaacatgcg tacctgcacc
    10801 gagatcctcg ctggcgctga caggccatga actgtcaccg acgcgcatat gaccgcgccc
    10861 gttgaccagc ggagattcca gcacaaaacg tcggccaatc agctgctgcc cgcgctggtt
    10921 taaatgacta tcgctgtgct tttgttcgcg cacccgccgc gacaaccatt tccaccacag
    10981 ccaggcggcg agcagcgtca ggatggcaaa cattacccct tgccactccc aacccagcgg
    11041 caccagccag accaccaggc cggtaatcac cgctgccacg ccgctccaca acaaataacc
    11101 atttccgccc agcatctcgg ctgccagcag caaaccgccg agactgagcc agaaaatatg
    11161 tggatgaacg accattaact ccatcatggc tgagtccgtt tgttggcgct gtctttcacc
    11221 agctcggcaa tcccggcaat cgaccccatc aggctgctgg cctctaatgg catcatcact
    11281 actttgctgt tactggaggt accgatctgc tgtaacgctt cggtgtattt ctgcgctacg
    11341 aagtagttca ccgcctgaat atcaccggag gcgatagctt cagacaccat tttggtggcg
    11401 cgggcttctg cttcggcgga acgttcacgc gcttcagcct gtaaaaacgc cgactgacgt
    11461 tcgccttccg ctttcaggat ttgcgactgt ttttcacctt cggctttgag gatttccgcc
    11521 tgacggatcc cttccgcttc aagaatgtaa gcgcgtttgg tacgttccgc tttcatctgc
    11581 gcgttcattg aagagataag ctctgccggt gggcgcacgt cgcgaatttc aatacgggtg
    11641 actttaatcc cccacgggtt ggtggcttca tcgacaatac gcagcaggcg tgagttgatg
    11701 ctgtcgcgct gagagagcat ttcgtcaagt tccattgaac ccaacacggt acggatgtta
    11761 gtcatggtca ggttgatgat cgccagctcc agattgctga cttcataagc cgcgcgcggc
    11821 gcgtcaatca cctgaataaa acagactgcg tcgatggtaa cgttggcgtt atctttcgag
    11881 ataacttcct gggaagggat atcgagcact tgctccatca tattgatctt gcgaccaatg
    11941 cgatccataa acggcaccac cagactgagc cccggctgta acgttttggt atagcgacca
    12001 aaacgttcta ccgtccattg atagccctgc ggcacgattt tgacgcccgc gccgacaatg
    12061 accagcgcga caaaaatgag aatcgggata aagataagca tcggaaaaac ctcctgttgt
    12121 accgtccata atcagcaaaa ttgctgcttg attaaacaaa ttatacctga ttactgaaag
    12181 agagttcccc cttattcctg cgaaggataa actgttttta gtaaaaatca gaaaaaggga
    12241 acagcgatgc aggaaaatag tcctttgctt cagctacaaa acgtaggata tctggcgggt
    12301 gatgcgaaga ttcttaataa catcaatttt tcgctgcgtg ctggcgaatt taagttaatt
    12361 accggtcctt ctggttgtgg caaaagtacg ctgctaaaaa tagttgcttc attgatcagc
    12421 ccaaccagcg gaacgttact gtttgaaggt gaggatgtca gcacactaaa gccagaaatc
    12481 taccgccaac aagtctctta ctgcgcccag acaccgacgc tgtttggcga tacggtatac
    12541 gataatctga tctttccctg gcagatccgt aaccggcagc ctgacccagc catttttctc
    12601 gattttctcg aacgcttcgc cttgccggac agcattttga cgaaaaatat cgccgagcta
    12661 tctggtggtg aaaaacaacg catctcactg attcgtaacc tgcaatttat gccgaaggtt
    12721 ttattgctgg atgaaatcac cagtgcgctg gatgaaagta ataaacataa cgttaatgag
    12781 atgatccatc gttatgtgcg cgagcaaaat attgccgtgc tgtgggtgac acacgataaa
    12841 gacgaaatta atcatgcgga taaagtgatt acactgcaac cgcatgccgg agaaatgcag
    12901 gaagcacgct atgaactcgc ataatattac taacgaatca ttagcactgg cattaatgct
    12961 ggtggtggtg gcaatcttaa ttagccataa agaaaaactg gcgctggaga aagatattct
    13021 ctggagcgtc ggacgagcga taattcagct gattattgtc ggctatgtgc tgaagtatat
    13081 tttcagcgtg gatgatgcca gcctgacatt attgatggtg ttatttatct gctttaatgc
    13141 ggcgtggaac gcgcaaaaac gcagtaaata tattgctaaa gcttttatct catcgtttat
    13201 tgccattacg gtcggggcgg gaattaccct ggcggtgctg attctctcag ggtcgattga
    13261 atttatcccg atgcaggtga tccctatcgc cgggatgatt gccggtaacg ccatggtagc
    13321 ggtggggttg tgttacaaca atttagggca acgggtcatt agcgaacagc aacagattca
    13381 ggagaaactg agtcttggtg cgacgccgaa gcaggcttca gcgatattga ttcgcgacag
    13441 tattcgcgcg gctttaattc cgacggtcga ttcagcaaaa acggttggct tagtgagttt
    13501 accaggaatg atgtccgggc tgatatttgc cgggattgat ccggtgaagg cgattaaata
    13561 tcagattatg gtgaccttta tgctgctctc aaccgccagc ttgtcgacca ttattgcctg
    13621 ctatttaacc tatcgtaagt tttataattc gcgccaccag ttggtggtga cgcaattgaa
    13681 gaagaaatga tgcgatgccg gatgcgcagc atcgcatccg gcgttgtggt tcatgtgccg
    13741 atcaacggaa tatcaataca acaatgcata cagctggcgg cgatacttcg acgccagtgc
    13801 atcacccgta cccagcgcag cgaggatctc ctggaacgtt ttacgcgtct gaccgtctgc
    13861 ggcggtgaga tctttacgca gatgcccgaa cagcaactcc agcgcctctt cattgcgccc
    13921 aacctgatgc agttgcagcg ccagttgcgt cgccagtgcg gcatcttctg gattctccgc
    13981 cacctgctgt tgcaactgtt gaatttccgg cgtatcagcc gcctgcttca gcagttcgat
    14041 ttgcgccacc atcccctggt agcgggtgtc ctgatcctgt aacggaatgg ttttcagcac
    14101 cgcttctgca tcttcagaac ggttcagcgc aatcagcgtt tctgccaaca gcaggccgat
    14161 ctccccgttc tgattcgaca actgccaggc gtctttcagc aatggcaggg catcggtgaa
    14221 attgccttcc tgcatcagtt gcatcgcctg ctgcgctttc agctcttctt cgcgcggcag
    14281 cactttatcc agcagggcgc ggatcgcctc ttccggttgc ggcccctgga agccatctac
    14341 cggttgcccg ttctggaaca gatacaccgt cggaatcgcg cgcagaccaa actgggcggc
    14401 aatcatctgc tccgcatcgc agtccagctt cgccagaata aattgcccgt tgtactgcgc
    14461 cgcaaggctt tccagaattg gggttaactg caaacagtgc tggctacgtt cagaccaaaa
    14521 atagaacagc accggagtgg tcatcgactg ttcaagaacc tgttgcaggt tagattcgtt
    14581 aatgttgaca atattttcta cggacatgga gtcgctctct gttgtcgata ttttctttga
    14641 catgggggct taagcgcgcg cttcaactca cccctgcaat attttgtcca tcacgcgccc
    14701 cggcagcagg cgcttaagca ccattactgc ccaagtcacc agcgttaccg gatagcgcat
    14761 cttcggcttc tcgctaataa aagcatggcg tactttgtcc accaccgctt ccggtcccaa
    14821 cgtaaagcgg gcggcgatgc cgggattttc gactggttta tcactttgcg tctggttgac
    14881 gttgtcggtg aagcgagtac gaatgggacc gggttcgatc aggctgactt taattccgct
    14941 gtggcgtagc tccatgcgca gcgcgtctga ccatgcctcc agcgcatatt tactggccgc
    15001 gtaagcgcca cgacccggtg tggagattaa tcccatcacc gatgatgtca tcacaatacg
    15061 cccttcaccg tgcggtaaca tcgcgggtaa caggcgcatg gtgagctggt gtgcgccgaa
    15121 aaagttggcg gaaaactgct gttccatctg cgcgcggctg atggtggaaa gggggccata
    15181 catgccgaat ccggcattgt taaagatccc atacagacaa ttatcggtca gggcgatcac
    15241 ttcgtcggct gcgcgatcaa cactttctgg cgaatccaga tcgatcaaca cgccggtaaa
    15301 tcccatgctg ttcatacgct caacatcatc cggtttccgg caacctgcca gcacatgaaa
    15361 accctggcgt tttaattcga gcgcgctttc caggccaatt ccactggaac atccggtaat
    15421 taagaccgat ttttgcataa ctttacctgt caggatctcc gttgctttat gagtcatgat
    15481 ttactaaagg ctgcaactgc ttcgccatcc agtcggcaat aaacggctgg gcgtcgcggt
    15541 tgggatgaat accgtcatcc tgcatccatt gtggcttgag gtagacctct tccataaaaa
    15601 agggcagcag cggaacatca aactctttgg cgagtttggg gtaaatggcg ctaaaggctt
    15661 cattataacg gcgaccatag tttgcaggca gacgtatttg cattaacaat ggttcagcgt
    15721 tggcggcttt gacatcctgc aaaatctggc gcagcgtttg ctcggtttgc tgtggctgaa
    15781 aaccacgcaa accgtcattg ccgcccagtt caaccagcac ccaacgcggc tgatgctgtt
    15841 tcagcagagc cggaaggcgc gccagtcctt gttgcgaggt gtcgccgctg atgctggcat
    15901 taactaccga cgttttactc tgccacttat cattcaacaa ggcaggccag gccgcgctgg
    15961 cagacattcg atacccggcg ctcaggctat cacccagaat caataacgtg tccgctgcgg
    16021 cggcacggaa ggttaacagg gccaggaaca ggaagggcaa atgccagcgg aaaacattgt
    16081 tgaagttcat catcttaaga agtccgtcgg tcagggggag catgaactct ccatcctcac
    16141 cggagttgag ctggttgtca aacgtggcga gaccatcgca ctggtgggcg agtcgggatc
    16201 gggtaagtca accttgctgg cgatcctcgc cgggcttgat gacggcagca gtggcgaagt
    16261 gagtctggtg ggacaaccgc tacataatat ggacgaagaa gcgcgggcaa agttgcgcgc
    16321 gaagcacgtc ggctttgttt ttcagtcatt tatgttaatt cctaccctta acgcgctgga
    16381 aaacgtcgag cttccggctc tgctgcgcgg tgagagtagc gcggaaagtc gtaacggggc
    16441 gaaagcgttg ctcgaacagt tagggctggg taaacgtctg gatcatcttc cggcacagct
    16501 ttccggcggt gaacagcaac gagtggcgct ggcacgagcc tttaatggtc gacctgatgt
    16561 gctgtttgcc gacgaaccca ccggcaacct tgaccgccag acgggcgata aaattgccga
    16621 cctgctgttt tccctcaacc gtgaacatgg caccacgttg attatggtga cccacgacct
    16681 gcaactggcg gcacgctgcg accgctgctt acggctggtg aacgggcagt tgcaggagga
    16741 agcatgattg cacgttggtt ctggcgcgaa tggcgttcgc cgtcgctatt aattgtctgg
    16801 ctggcgctaa gcctggcggt ggcctgcgtg ctggcgctgg gcaatatcag cgatcgcatg
    16861 gagaagggct taagccagca aagccgtgag tttatggcgg gcgatcgggc gttgcgcagt
    16921 tcacgcgaag tgccgcaagc gtggctggag gaagcgcaaa agcgcggcct gaaagtcggc
    16981 aagcagctga ctttcgccac aatgaccttt gcaggcgaca caccgcagct ggcgaacgtc
    17041 aaagcggtgg atgatatcta cccgatgtat ggcgatctgc aaactaatcc ccctggcctg
    17101 aaaccgcagg cgggcagcgt attgctggcc ccacgcctga tggcactgct taacctgaaa
    17161 acgggcgaca ccattgacgt gggcgatgcc accttgcgga ttgccggaga agtgattcag
    17221 gaaccggatt ccggttttaa ccccttccag atggctccgc gtctgatgat gaatctggcg
    17281 gatgtcgata aaaccggagc cgtgcagccg gggagtcggg tcacctggcg ttataaattc
    17341 ggcggcaacg agaaccagct cgacggctat gataaatggt tgttacctca gcttaaaccc
    17401 gaacaacgct ggtacggtct ggaacaggac gaaggcgcgc tggggcgatc gatggaacgc
    17461 tcgcaacagt tcctgctgct ttcggcgctt ctgaccttgc tgctggcagt ggcagcggtg
    17521 gcggtagcga tgaatcatta ctgtcgcagt cgctacgatc tggtggcgat cctcaaaacg
    17581 ctgggggcag ggcgagcgca actgcgtaag ctaatcgtcg gtcagtggtt gatggtgctg
    17641 acgctttcag ccgttaccgg tggggccata ggcctgttgt tcgaaaacgt gttgatggtg
    17701 ctgctcaagc ccgttctgcc tgctgcacta ccgccagcca gcctctggcc gtggctgtgg
    17761 gcgcttggca ccatgacggt catctcgctg ctggtggggc tacgaccata ccgcttgttg
    17821 ctggcaacgc agcctttacg cgtattacgt aatgatgtgg tagcgaacgt ctggccgctg
    17881 aagttttatc tgccgattgt cagtgtggtg gttgtgctgc tgctcgccgg attaatgggt
    17941 ggcagcatgc tgctttgggc ggtgctggcg ggcgcggtag tactggcttt gctgtgcggt
    18001 gtgctgggct ggatgcagct gaatgtactt cgccgcatga cgctgaaatc gctacctctg
    18061 cgcctggcgg ttagccgcct gttacgtcag ccgtggtcaa cgttaagcca gctttcggca
    18121 ttttcgctct cctttatgct gctggcgctg ttgctggtat tgcgtggcga tctgctcgac
    18181 cgctggcagc agcagctacc gccagaaagc ccgaactact ttttaattaa catcgccaca
    18241 gaacaggtag caccgctaaa agcgttcctc gcggaacatc atatagtccc ggaatcattt
    18301 tatccggtag tgcgggcgcg gctgacggcg attaacgata agccgacaga aggtaatgaa
    18361 gatgaggcgc ttaatcgcga actcaatctt acctggcaaa atacgcggcc cgatcataat
    18421 ccgattgtcg ccggtaactg gccgccaaaa gccgatgaag tgtcgatgga agaggggctg
    18481 gcgaaacgct taaacgttgc cctcggtgat accgtgactt ttatgggcga tacccaggag
    18541 ttccgcgcta aagtgaccag cctgcgcaaa gtggactggg aaagtctgcg gcctaatttc
    18601 tattttattt tcccggaagg ggcattagac gggcaaccgc agagctggct taccagtttc
    18661 cgctgggaga atggcaacgg catgttgacg caactcaacc gccagttccc gaccattagc
    18721 ctgttagata ttggcgcgat tttaaaacag gtcggtcagg tgctggagca ggtaagtcgg
    18781 gcgctggagg tgatggtggt gctggtcacc gcctgcggta tgttgctgtt gctggcacag
    18841 gtgcaggtgg gaatgcgtca gcgtcatcag gagctggtgg tgtggcgcac actcggtgcg
    18901 gggaaaaaac tgctgcgtac cacgttgtgg tgtgagttcg ccatgcttgg gtttgtttct
    18961 ggcctggtag ccgcaattgg tgcggaaacg gcgctggcag tgttgcaggc gaaagtgttt
    19021 gatttcccgt gggagccaga ctggcgattg tggattgttc tgccgtgcag cggtgcactg
    19081 ctgctgtcgc ttttcggcgg ctggctgggt gcgcgactgg ttaagggtaa ggcgctgttc
    19141 aggcagtttg cggggtgatg acaggtgtta gattgcctga tgcgctacgc ttatcaggcc
    19201 tacgaagagc gtgcaatata ttgattttgc aagattgtgt aggccggata aggcgtttac
    19261 gccgcatctg gcatgaacag tgtgcatttt ggcaataaat atgaaaccaa ctgtcatgtc
    19321 ggttttttat atctgtattc tggtgcaacc attctgttct cacgacaatc gccgtttcag
    19381 aataccgccc atcactgcgt cttaacccat tccgccactt ccgcccactc accgcgaaag
    19441 acaacttttt ccgctttttt ctcaagctga tagcgataca tcgggtcgta atattcttta
    19501 agtaacggca ccagccaggc cagatgaccg tcggtgctgt cggtggtgag ttgcgttgtc
    19561 agcgccgtat ccagctgggc agcgagttcg ttatagcgct gtaaccccag ccgacgctta
    19621 atagccgaaa gtccgtgatg caggtattcg caatattcct gccagccctg ttcgtcgccg
    19681 tacgcgtggg taaaatcatg atgcatacgc aagaaatact cttcgttcag gcgctcaaga
    19741 cggatctcaa acggatcttc taccaccgca atcgccgcct gagtcattcg ctcgcgcagg
    19801 cattccggca ggtgattcga accgatcatc cggctttcgt cttccagcac ccacaggcgc
    19861 aaatcctgac gggcgtcggt ttttagcatt tcggcagcca gcaggttttc aaaactcgcc
    19921 tggctaagtt gtggttgtaa ggtgcgacca aacgccgaac cgcgatgacg cgctaaccct
    19981 tccagatcaa caccgttcgg ctgttgctgc actaacagcg ttttaccgct gccggtacaa
    20041 ccgccaatca gcactatcgg tttttgtgcc agttcaatag tcgcctgaat cgcggcttgg
    20101 cgcagtgcct tataaccgcc ttccaccagc ggataatcaa tccccgctgc atacaaccag
    20161 ctttgcacaa tatgtgagcg ctgaccgcca cgggcgcagc agagaatacc ttgcggattt
    20221 tgcaggcacg ctgcccgcca ggcgtccatg cgctgctgac gaatttcacc cgccaccagt
    20281 ttatgcccca gcgccagcgc cgcgtctgag ccttgctgtt tataacaagt gccaacggcg
    20341 gcgcgttcat cgttattcat taacggcagg ttgatagcgg cgggcattgc gccttgctca
    20401 aactcgatag gggcgcgaac atcaataatg ggcgtatcag caatcagcag ggcacgatag
    20461 tcctgttccg tgtgtctctc ttgcatagtt aaaagtgaac ctcaaatcag cttgcgcgct
    20521 attttacgcg ccaacgcgca aggaaacttg atttttaact gcgtgggttg ccgaaaattt
    20581 ctaaaaatcc gctgatttcc ggcctgcgct gggtaaacaa ggtcacaata tcttctaccg
    20641 ctttgccgct gccaaatttg cgccatgcca gactcaatgg cgaaggaggg cgcatcgttg
    20701 ggattacccg gctgaccagt tgttgattat cgatcattga ctggcaaagt gattttggca
    20761 aaaaaccaat gccaacaccc gccagatggg cggcgatttt cgtttccata tcgggaacaa
    20821 taatctcttt ttgccccggc aatcgccagg cgacgcgttt ggttaaggtt cgggcgctgt
    20881 cttcaatatt gaccgccgga aagcgccgca actgcgcttc tgttagcggc tcttcaacgt
    20941 tcgccagcgg atgatccgcc gccatgacaa agcgccattg caccgatcct aagggatcaa
    21001 gactaaaggt atttgccagc gcctcagttc ccgtgacccc gatagccagc gaaaaacctt
    21061 cgtacaatag cgagtcccag acgcccatat agatttgtcg ggagatgtga aactgggtaa
    21121 aggggtaacg ctcattcagc cacgccagca actgggcgac ggcctggggg ttatagagca
    21181 ggttgttgat gacaatattc acctagcgtt ccacgccatc attcacctgt tgcagctcgc
    21241 tgggcatact ttccagccag ctcagccagt ctctggcctg ggaaagtaga tgctcgccag
    21301 ccgctgtcaa cgtcacgctg cgagtcgtgc ggaaaaacag cgccactccg gtattctctt
    21361 ccagaagttt aatgcgataa ctgatcgtcg ccgtggtttt acataatcgt tctgccgctt
    21421 ttgaaaaact tcctgtttca gcaaccgcaa tgaaagtccg caaggtttct ggatcgaaca
    21481 tcttcaggta tcccctttta aatccgcaag ttgcgtgatt ttcttatcct ctgatttatc
    21541 agtattttta catgataacc ctgttcaatt tgtggactaa atctagtttt ggaaaaatat
    21601 tccaactttt gtattgatgt tgttctctta aggttttaga t
//
LOCUS       DADMKF010000062        21395 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.063, whole genome shotgun sequence.
ACCESSION   DADMKF010000062 DADMKF010000000
VERSION     DADMKF010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21395
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.063"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            21..2393
                     /locus_tag="JNC24_002136"
     CDS             21..2393
                     /locus_tag="JNC24_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HAY9158877.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMHFFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSKVKLGYRTTRSDWLTSHIDENNKSAFNQGSSGSTYYSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVTGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTCGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGDVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMAEHISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQDLKTPYNDELAMGLQQKIGKNVIARAN
                     YVYREAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFSLSFELAEPLHIRQVDINPQ
                     IVFSYIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFT
                     HQPSGLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSW
                     TPQFLKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            2438..5233
                     /locus_tag="JNC24_002137"
     CDS             2438..5233
                     /locus_tag="JNC24_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAY9158878.1"
                     /translation="MEIIMRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLDNGL
                     RYMIYPHAQPKDQVNLWLQIHTGSLQEEDNERGVAHFVEHMMFNGTKTWPGNKVIETF
                     ESMGLRFGRDVNAYTSYDETLYQVSLPTTQKQNLQQVMAIFSEWSNAATFEILEVDAE
                     RGVITEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQR
                     WYQPNNMTFIVVGDIDSKEALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDK
                     ENRVNGIALYYRLPMVQVNDEQSFVEQAEWSMLVQLFNQRLQERIQSGELKTISGGTA
                     RSVKIAPDYQSLFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWL
                     KNAVDQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQL
                     RKNQDAFWEQMVNNELAAKKALSLAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQ
                     AEISSKETLAENLTSLTLSNGARVILAKSAGEEQKLQITAVSNKGDLSFPAQQKSLIA
                     LANKAVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLIN
                     QRITHSTINDNIWASLQNAQIQALKTLDQRPAEKFAQQMYETRYADDRTKLLQENQIV
                     QFTAADALAADRQLFSSPADITFVIVGNVSEDKLVALITRYLGSIKHSDSPLAAGKPL
                     TRATDNASVTVKEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIRE
                     QASGAYSVSSRLSVDPQAKDISHLLAFTCQPERHDELLTLANEVMVKRLAKGISEQEL
                     NEYQQNVQRSLDIQQRSVQQLANTIVNSLIQYDDPAAWTEQEQLLKQMTVENVNTAVK
                     QYLSHPVNTYTGVLLPK"
     gene            5595..6995
                     /locus_tag="JNC24_002138"
     CDS             5595..6995
                     /locus_tag="JNC24_002138"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HAY9158879.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVPTFGVTYTGNYE
                     FPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPDIVWDFRLPRVKSISASGHK
                     FGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRL
                     GREGYTKVQNASYQVAAYLADEIAKLGPYEFICAGRPDEGIPAVCFKLKEGEDPGYTL
                     YDLSERLRLRGWQVPAFTLGGEATDIVVMRIMCRRGFEMDFAELLLEDYKASLKYLSD
                     HPKLQGIAQQNSFKHT"
     gene            7151..8686
                     /gene="gadC"
                     /locus_tag="JNC24_002139"
     CDS             7151..8686
                     /gene="gadC"
                     /locus_tag="JNC24_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HAY9158880.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            8817..10136
                     /locus_tag="JNC24_002140"
     CDS             8817..10136
                     /locus_tag="JNC24_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HAY9158881.1"
                     /translation="MDICSRNEKLAIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            10508..11890
                     /gene="dosC"
                     /locus_tag="JNC24_002141"
     CDS             10508..11890
                     /gene="dosC"
                     /locus_tag="JNC24_002141"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HAY9158882.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPLIRAKAAEIAVAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
                     SASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKILLDSDLGSSLPLSQADFG
                     LWFNHKGRHYFSGIAEVGHISRLIQDFDGIFNQTMRNTRNLNNRSLRVKFLLQIRNTV
                     SQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDV
                     DKFKEINDTWGHNTGDEILRKVSQAFYDNVHSSDYVFRYGGDEFIIVLTEASENETLR
                     TAERIRSRVEKTKLKAANGEDIALSLSIGAAMFNGHPDYERLIQIADEALYIAKRRGR
                     NRVELWKASL"
     gene            11915..14314
                     /gene="dosP"
                     /locus_tag="JNC24_002142"
                     /gene_synonym="pdeO"
     CDS             11915..14314
                     /gene="dosP"
                     /locus_tag="JNC24_002142"
                     /gene_synonym="pdeO"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-sensing cyclic-di-GMP phosphodiesterase"
                     /protein_id="HAY9158883.1"
                     /translation="MKLTDAENAADGIFFPALEQNMMGAVLINENDEVMFFNPAAEKL
                     WGYKREEVIGNNIDMLIPRDLRPAHPEYIRHNREGGKARVEGMSRELQLEKKDGSKIW
                     TRFALSKVSAEGKVYYLALVRDASVEMAQKEQTRQLIIAVDHLDRPVIVLDPERHIVQ
                     CNRAFTEMFGYCISEASGMQPDTLLNIPEFPADNRIRLQQLLWKTARDQDEFLLLTRT
                     GEKIWIKASISPVYDVLAHLQNLVMTFSDITEERQIRQLEGNILAAMCSSPPFHEMGE
                     IICRNIESVLNESHVSLFALRNGMPIHWASSSHGAEVQNAQSWSATIRQRDGAPAGIL
                     QIKTSSGAETSAFIERVADISQHMAALALEQEKSRQHIEQLIQFDPMTGLPNRNNLHN
                     YLDDLVDKAVSPVVYLIGVDHIQDVIDSLGYAWADQALLEVVNRFREKLKPDQYLCRI
                     EGTQFVLVSLENDVSNITQIADELRNVVSKPIMIDDKPFPLTLSIGISYDVGKNRDYL
                     LSTAHNAMDYIRKNGGNGWQFFSPAMNEMVKERLVLGAALKEAISNNQLKLVYQPQIF
                     AETGELYGIEALARWHDPQHGHVPPSRFIPLAEEIGEIENIGRWVIAEACRQLAEWRS
                     QNIHIPALSVNLSALHFRSNQLPNQVSDAMQAWGIDGHQLTVEITESMMMEHDTEIFK
                     RIQILRDMGVGLSVDDFGTGFSGLSRLVSLPVTEIKIDKSFVDRCLTEKRILALLEAI
                     TSIGQSLNLTVVAEGVETKEQFEMLRKIHCRVIQGYFFSRPLPAEEIPGWMSSVLPLK
                     I"
     gene            14573..15154
                     /gene="ddpX"
                     /locus_tag="JNC24_002143"
     CDS             14573..15154
                     /gene="ddpX"
                     /locus_tag="JNC24_002143"
                     /EC_number="3.4.13.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
                     /protein_id="HAY9158884.1"
                     /translation="MSDTTELVDLAVIFPDLEIELKYACADNITGKAIYQQARCLLHK
                     DAITALAKSISIAQLSGLQLVIYDAYRPQQAQAMLWQACPDPQYVVDVTVGSNHSRGT
                     AIDLTLRDEHGNILDMGAGFDEMHERSHAYHPSVPPAAQRNRLLLNAIMTGGGFVGIS
                     SEWWHFELPQAASYPLLADQFSCFISPGTQHVS"
     gene            15168..16718
                     /locus_tag="JNC24_002144"
     CDS             15168..16718
                     /locus_tag="JNC24_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAY9158885.1"
                     /translation="MKRSISFRPTLLALVLATTFPVAHAAVPKDMLVIGKAADPQTLD
                     PAVTIDNNDWTVTYPSYQRLVQYKTDGDKGSTDVEGDLASSWKASDDQKEWTFTLKDN
                     AKFADGTPVTAEAVKLSFERLLKIGQGPAEAFPKDLKIDTPDEHTVKFTLSQPFAPFL
                     YTLANDGASIINPAVLKEHAADDARGFLAQNTAGSGPFMLKSGQKGQQLVLVPNPHYP
                     GNKPNFKRVSVKIIGESASRRLQLSRGDIDIADALPVDQLNALKQENKVNVAEYPSLR
                     VTYLYLNNSKAPLNQADLRRAISWSTDYQGMVNGILSGNGKQMRGPIPEGMWGYDATA
                     MQYNHDETKAKAEWDKVTSKPTSLTFLYSDNDPNWEPIALATQSSLNKLGINVKLEKL
                     ANATMRDRVGKGDYDIAIGNWSPDFADPYMFMNYWFESDKKGLPGNRSFYENSEVDKL
                     LRNALATTDQTQRTRDYQQAQKIVIDDAAYVYLFQKNYQLAMNKEVKGFVFNPMLEQV
                     FNINTMSK"
     gene            16720..17742
                     /locus_tag="JNC24_002145"
     CDS             16720..17742
                     /locus_tag="JNC24_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY9158886.1"
                     /translation="MTFWSILRQRCWGLVLVVAGVCVITFIISHLIPGDPARLLAGDR
                     ASDAIVENIRQQLGLDQPLYVQFYRYFSDLFHGDLGTSIRTGRPVLEELRIFFPATLE
                     LAFCALLLALLIGIPLGILSAVWRNRWLDHLVRIMAITGISTPAFWLGLGVIVLFYGH
                     LQILPGGGRLDDWLDPPTHVTGFYLLDALLEGNGEVFFNALQHLILPALTLAFVHLGI
                     VARQIRSAMLEQLSEDYIRTARASGLPGWYIVLCYALPNALIPSITVLGLALGDLLYG
                     AVLTETVFAWPGMGAWVVTSIQALDFPAVMGFAVVVSFAYVLVNLVVDLLYLWIDPRI
                     GRGGGE"
     gene            17739..18635
                     /locus_tag="JNC24_002146"
     CDS             17739..18635
                     /locus_tag="JNC24_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HAY9158887.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLML
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLISWHILRNSLPPLIVPA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTAV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            18632..19618
                     /locus_tag="JNC24_002147"
     CDS             18632..19618
                     /locus_tag="JNC24_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAY9158888.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGYVHVLNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGDDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAITLLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIADEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGVTADVIHHPRHPYTIGLLQCAPEHGVPRQPLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCENVPALTACGDNNQRCACWYPQQEVISI"
     gene            19647..20537
                     /locus_tag="JNC24_002148"
     CDS             19647..20537
                     /locus_tag="JNC24_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAY9158889.1"
                     /translation="MNFPARKSWLGKTTEHVHAINGIDLQIRRGETLGIVGESGCGKS
                     TLAQLLMGMLQPSHGQYIRSGSQRIMQMVFQDPLSSLNPRLSVWRIITEPLWIAKRSS
                     EQQRRALAKELAVQVGIRPEYLDRLPHAFSGGQRQRIAIARALSSQPDVIVLDEPTSA
                     LDISVQAQILNLLVTLQENHGLTYVLISHNVSVIRHMSDRVAVMYLGQIVELGDAQQV
                     LTAPAHPYTRLLLDSLPAIDKPLEEEWALCKTDLPGNRTLPQGCFFYERCPLATHGCE
                     VRQSLAIREDGRELRCWRAL"
     gene            complement(20593..21024)
                     /gene="osmC"
                     /locus_tag="JNC24_002149"
     CDS             complement(20593..21024)
                     /gene="osmC"
                     /locus_tag="JNC24_002149"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HAY9158890.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTQSGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDADFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
BASE COUNT         5545 a         5190 c         5277 g         5383 t
ORIGIN      
        1 ctgtacggag atataaataa atgaagcgag ttcttattcc tggcgtcatt ttatgtggcg
       61 ctgatgtggc gcaggccgtc gatgacaaaa acatgtacat gcattttttt gaagagatga
      121 cggtctatgc tcctgtccct gtacccgtaa acggcaacac gcattacacc agtgaaagca
      181 tcgagcgttt accgaccggg aatggcaata tcagcgatct gctgagaacc aaccctgcgg
      241 tacgcatgga ttcaacgcaa agtacctcgt tgaaccaggg agatattcgc cctgagaaaa
      301 tctctattca cggtgcgtcg ccctaccaga atgcctattt gattgacggt attagtgcaa
      361 ctaataacct gaacccagcg aatgagtccg atgccagtag tgcaaccaat attagcggga
      421 tgtcacaggg gtattatctt gatgtcagct tactggacaa tgtgacgctt tatgacagtt
      481 ttgtgccggt tgaatttggt cgcttcaatg gcggggtaat tgatgcaaag atcaaacgct
      541 tcaacgctga tgatagcaag gtgaaattgg gttatcgcac tacgcgttcg gactggttaa
      601 catcgcatat cgatgagaat aacaagagcg catttaatca aggctcttca ggaagtactt
      661 attactcccc agattttaaa aagaactttt ataccttgtc gtttaatcag gaactcgctg
      721 ataacttcgg cgttaccgcc ggtttatcgc gccgccagtc tgatatcacc cgcgcggatt
      781 atgtttcgaa tgacggcatt gtcaccggtc gggcacagta taaaaacgtt atcgatactg
      841 cattgagcaa atttacctgg tttgccagcg accgctttac ccacgattta accttaaaat
      901 ataccggctc cagccgtgat tataatacca gcaccttccc gcagtctgat cgcgaaatgg
      961 gtaataaatc ctatggtctg gcatgggata tggatacgca actcgcatgg gccaaactac
     1021 gtaccaccgt tggttgggat catattagtg attatacccg tcacgatcat gacatctggt
     1081 acaccgaact ttcatgtaca tatggtgata ttacagggcg ttgtacctgt ggcggattag
     1141 gacacatttc ccaggctgta gataattaca ccttcaaaac acgcctggac tggcaaaaat
     1201 tcgccgtggg tgatgtttcg catcaaccct acttcggcgc ggaatacatc tattccgatg
     1261 catggactga acgccataac cagtctgaat cctatgtgat taatgctgcc ggaaagaaaa
     1321 ctaaccatac catttaccat aaaggtaaag gcagcctggg aattgacaac tacacgctgt
     1381 atatggcgga gcacattagc tggcggaatg tgtcgttaat gcccggtgtg cgttatgact
     1441 atgacaacta tctgtcaaac cacaatatct ccccgcgctt tatgacggaa tgggatattt
     1501 ttgctgatca aacctcaatg attaccgccg gttataaccg ttactatggc gggaatattc
     1561 ttgatatggg attacgtgat atccgcaata gctggacgga atcggtatca ggtaataaaa
     1621 ctctgacgcg ttatcaggat ttgaaaacgc cttataacga tgaactggca atgggattgc
     1681 agcagaaaat cggtaagaac gttattgcgc gtgcaaacta tgtttaccgt gaagcgcatg
     1741 atcaaatcag caaaagcagt cgtaccgaca gcgcgactaa aaccaccatt actgaatata
     1801 acaacgatgg caaaaccaaa acgcattcgt tcagcctcag ttttgaactg gccgaacccc
     1861 tgcatatccg ccaagtagat attaacccac aaattgtctt tagctatatc aagagcaagg
     1921 gcaacttgtc gttaaacaat ggttatgagg agagcaatac cggtgataac caggtggttt
     1981 ataacggtaa tctggtctct tacgatagtg ttccagtggc agattttaat aacccattaa
     2041 agatctcctt aaacatggat ttcacgcatc aaccgagcgg gttagtgtgg gcgaatacgc
     2101 tggcctggca agaagcgcgt aaagctcgca ttatccttgg taagacaaat gcgcaataca
     2161 tcagcgaata ttcagattac aagcagtatg ttgacgaaaa actggatagc agcctgacct
     2221 gggacacccg cttgtcctgg acgccacaat ttctgaaaca acaaaacctg acgatcagtg
     2281 ccgatattct caatgtactg gatagcaaaa ccgctgttga tacaacgaac accggtgtgg
     2341 cgacctacgc cagtggccgt actttctggc ttgatgtcag catgaaattt taatagttcg
     2401 gattttactt aaccgggtaa attcgcccgg ttttcgcatg gagataatta tgagaaacct
     2461 ctgtttctta ctgacgttag tggcaactct gttgctcccc gggcggctga ttgccgccgc
     2521 cttaccgcag gatgaaaagt taattaccgg gcaactggac aatggcttgc gatatatgat
     2581 ttatccgcat gctcaaccaa aggatcaggt aaatttatgg ctgcaaattc ataccggttc
     2641 attgcaggaa gaagacaatg agcgcggcgt ggctcatttt gtagaacata tgatgtttaa
     2701 cggcacaaaa acatggccgg gtaataaagt catcgaaaca tttgagtcaa tgggcctgcg
     2761 tttcggtcgc gatgttaatg cctataccag ctatgacgaa acgctgtatc aggtgagtct
     2821 gccgactacg cagaaacaaa atctgcaaca agtgatggca atcttcagtg aatggagtaa
     2881 tgccgcaacc tttgaaatac tcgaagtaga cgctgaacgc ggcgtaatta ctgaggaatg
     2941 gcgtgcccat caggatgcga aatggcgcac ctctcaggcg cgccgccctt tcctgctggc
     3001 aaatacccgt aatttagacc gtgaacctat cggcctgatg gatactgtcg ccacggtcac
     3061 accggcacaa ttgcgccaat tttatcaacg ctggtatcaa ccaaataata tgacctttat
     3121 cgtggtcggc gatatcgaca gtaaagaagc gctggcgctg ataaaggata atttaagtaa
     3181 gcttccggct aacaaagcag ctgaaaatcg cgtctggccg acaaaagccg aaaaccacct
     3241 gcgctttaat atcattaatg ataaagaaaa ccgagtgaac ggcatcgcac tctattatcg
     3301 cctgccaatg gtacaagtga acgatgagca aagctttgtc gaacaagctg aatggagcat
     3361 gttagttcag ctgttcaatc aacgtctgca ggaacgcata cagtcgggcg agttgaagac
     3421 tatttctggc ggcactgcgc gcagcgttaa aattgcaccc gattatcagt cgctgttttt
     3481 ccgtgtaaat gcacgagacg ataatatgca ggatgctgca aatgcattaa tggcagagtt
     3541 ggcaaccatt gatcagcatg gtttttctgc tgaagaactc gatgatgtca aatctacccg
     3601 cctcacctgg ctgaaaaatg cggttgatca gcaagctgaa cgtgatttac gtatgctgac
     3661 cagtcgcctg gcatccagct cattaaataa tacgccgttc ttgtcgccgg aagagacata
     3721 tcaactttcg aaacgtctgt ggcagcaaat taccgtgcaa agtctggcgg aaaaatggca
     3781 gcagttaaga aagaaccagg acgcattttg ggagcaaatg gtaaacaatg agcttgccgc
     3841 caaaaaagca ttgtctcttg cggctatcct ggcgctggaa aaagagtacg ccaacaaaaa
     3901 gctggcggct tacatcttcc caggcagaaa tttatcgtta acagtagacg ctgacccaca
     3961 ggcggaaatt agcagcaaag aaacgctggc tgagaatctg acatcattaa cactttccaa
     4021 tggtgccagg gttattctgg caaaatccgc gggtgaagag caaaagctac aaattactgc
     4081 cgtatctaat aaaggcgatt taagtttccc tgcgcagcaa aaatcactta tcgcgctggc
     4141 aaataaagca gtaagcggaa gcggcgttgg tgagctctcc tcttccagcc tgaaacgctg
     4201 gagtgcggaa aattcggtaa ccatgagcag taaagtcagt ggcatgaata cgttgctctc
     4261 cgttagcgcg cggactaata accctgaacc tggttttcag ttgattaacc agcgaatcac
     4321 ccacagcacg attaacgaca atatttgggc atcgctacaa aatgctcaaa ttcaggcgtt
     4381 gaaaacgctc gaccagcgtc cagcggagaa attcgcccag cagatgtatg agacgcgcta
     4441 tgctgatgac cgcacgaaat tactgcaaga aaatcagatt gtacagttta ctgccgcaga
     4501 tgcgctggct gccgatcgcc agttgttttc atctccagcg gatatcacgt ttgtcattgt
     4561 cggtaatgtc tcagaagaca aactcgtggc gttaattacg cgttacttag gatcaatcaa
     4621 acactctgat tcgccattag ccgcaggtaa accattaact cgcgcgacgg acaacgcatc
     4681 ggttactgta aaagaacaaa atgaacctgt ggcacaggtt tcacagtgga agcgttatga
     4741 ttcccggaca cctgttaatc tggcgacgcg tatggcgctc gatgctttta acgtcgcact
     4801 ggcaaaagat ctacgcgtta atattcgtga acaggcatct ggagcataca gcgtttcttc
     4861 tcgcctctcg gttgatcctc aggccaaaga tatcagtcat ttgctggctt ttacttgtca
     4921 accagaacga catgatgaac tgttaacgtt agcgaatgaa gtgatggtta agcgtctggc
     4981 taaagggatc agtgagcaag aactgaatga ataccagcaa aacgttcagc gcagcctcga
     5041 tatccaacag cgtagcgttc aacaattagc gaacactatt gtaaatagtc ttattcaata
     5101 tgacgatcct gcagcatgga ctgagcagga gcaattgttg aaacaaatga cggtagagaa
     5161 tgttaacact gccgttaaac aatatctttc tcatccggta aatacttata ctggagtatt
     5221 attgccaaaa taataacagc cccgtcaaca catcgttggc ggggatttta gcaatattcg
     5281 ccatttttat gtaataattt tataaatgcg ttcaaaataa taaccaggta ctaatagtga
     5341 tattttaagg tctgattttt acgtgaaaat tcaggaggca cagaatgcgc ataaaaataa
     5401 cagcataaaa caccttacca ccacccaaga atttcatatt atattgtttt tcaatgaaaa
     5461 aatattattc tcgtaatatc tcacgataaa taacattagg attttgttat ttaaacacga
     5521 gtcctttgca cttgcttact ttatcgataa atcctacttt tttaatgcta tccaatcatt
     5581 ttaaggagtt taaaatggat aagaagcaag taacggattt aaggtcggaa ctactcgatt
     5641 cacgttttgg tgcgaagtct atttccacta tcgcagaatc aaaacgtttt ccgctgcacg
     5701 aaatgcgcga cgatgtcgca ttccagatta tcaatgacga attatatctt gatggcaacg
     5761 ctcgtcagaa cctggccact ttctgccaga cctgggacga cgaaaatgtc cacaaattga
     5821 tggatttatc cattaacaaa aactggatcg acaaagaaga atatccgcaa tccgcagcca
     5881 tcgacctgcg ttgcgtaaat atggttgccg atctgtggca tgcgcctgcg ccgaaaaatg
     5941 gtcaggccgt tggcaccaac accattggtt cttccgaggc ctgtatgctc ggcgggatgg
     6001 cgatgaaatg gcgttggcgc aagcgtatgg aagctgcagg caaaccaacg gataaaccaa
     6061 acctggtgtg cggtccggta caaatctgct ggcataaatt cgcccgctac tgggatgtgg
     6121 agctgcgtga gatccctatg cgccccggtc agttgtttat ggacccgaaa cgcatgattg
     6181 aagcctgcga cgaaaatacc atcggcgtgg tgccgacttt cggcgtgacc tacaccggta
     6241 actatgagtt cccgcaaccg ctgcacgatg cactggataa attccaggcc gacaccggta
     6301 tcgacatcga catgcacatc gacgccgcca gcggtggctt cctggcaccg ttcgtcgccc
     6361 cggatatcgt ctgggacttc cgcctgccgc gtgtgaaatc gatcagtgct tcaggccaca
     6421 aattcggtct ggctccgctg ggctgcggct gggttatctg gcgtgacgaa gaagcgctgc
     6481 cgcaggaact ggtgttcaac gttgactacc tcggtggtca gattggtact tttgccatca
     6541 acttctcccg cccggcgggt caggtgattg cacagtacta tgaattcctg cgccttggtc
     6601 gtgaaggcta taccaaagta cagaatgctt cctaccaggt cgctgcctat ctggcggatg
     6661 agatcgccaa actggggccg tatgagttca tctgtgccgg tcgcccggac gaaggcatcc
     6721 cggcggtttg cttcaaactg aaagagggtg aagatccggg atacaccctg tacgacctct
     6781 ctgaacgtct gcgtctgcgc ggctggcagg ttccggcctt cactctcggc ggtgaagcca
     6841 ccgacatcgt ggtgatgcgc attatgtgtc gtcgcggctt cgaaatggac tttgctgaac
     6901 tgttgctgga agactacaaa gcctccctga aatatctcag cgatcacccg aaactgcagg
     6961 gtattgccca gcagaacagc tttaaacata cctgataacg tttaacggta acggtgtccc
     7021 gaaacgaacc cgtttcggga taatttccaa agtctgttca ctggcattag caacggaaaa
     7081 tattgttctg aatacgcttc agaacaaaac aggtgcggtt ccgacaggaa taccgtttta
     7141 gggggataat atggctacat cagtacagac aggtaaagct aagcagctca cattactcgg
     7201 cttttttgcc ataacggcat cgatggtaat ggctgtttat gaatacccta ccttcgcaac
     7261 atcgggcttt tcattagtct tcttcctgct attaggcggg attttatggt ttattcccgt
     7321 gggactttgt gctgcggaaa tggccaccgt cgacggctgg gaagaaggtg gtgtcttcgc
     7381 ctgggtatca aatactctgg ggccgagatg gggatttgca gcgatctcat ttggctatct
     7441 gcaaatcgcc attggtttta ttccgatgct ctatttcgtg ttaggggcac tctcctacat
     7501 cctgaaatgg ccagcgctga atgaagaccc cattaccaaa actattgcag cactcatcat
     7561 tctttgggcg ctggcattaa cgcagtttgg tggcacgaaa tacacggcac gaattgctaa
     7621 agttggcttc ttcgccggta tcctgttacc tgcatttatt ttgatcgcat tagcggctat
     7681 ttatctgcac tccggtgccc ccgttgctat cgaaatggat tcaaagacct tcttccctga
     7741 cttctctaaa gtgggcaccc tggttgtatt tgttgccttc attttgagtt atatgggcgt
     7801 agaagcatcc gcaacccacg tcaatgaaat gagcaaccca gggcgcgact atccgttggc
     7861 tatgttactg ctgatggtag cggcaatctg cttaagctct gttggtggtt tgtctattgc
     7921 gatggtcatt ccgggtaatg aaatcaacct ctccgcaggg gtaatgcaaa cctttaccgt
     7981 tctgatgtcc catgtggcac cggaaattga gtggacggtt cgcgtgatct ccgcactgct
     8041 gttgctgggt gttctggcgg aaatcgcctc ctggattgtt ggtccttctc gcgggatgta
     8101 tgtcacagcg cagaaaaacc tgctgccagc ggcattcgct aaaatgaaca aaaatggcgt
     8161 accggtaacg ctggtcattt cacagctggt gattacttct atcgcgttga tcatcctcac
     8221 caataccggt ggcggtaaca acatgtcctt cctgatcgca ctggcgctga cggtggtgat
     8281 ttatctgtgt gcttatttca tgctgtttat tggctacatt gtgttggttc ttaaacatcc
     8341 tgacttaaaa cgcacattta atatccctgg tggtaaaggg gtgaaactgg tcgtggctat
     8401 tgtcggtctg ctgacttcaa ttatggcgtt tattgtttcc ttcctgccgc cggataacat
     8461 ccagggtgac tctaccgata tgtatgttga attactggtc gttagtttcc tggtggtact
     8521 tgccctgccc tttattctct atgctgttca tgatcgtaaa ggcaaagcga ataccggcgt
     8581 cactctggag ccaatcaaca gtcagaacgc accaaaaggt cacttcttcc tgcacccgcg
     8641 tgcacgttca ccacactata ttgtgatgaa tgacaagaaa cactaaccat gtctgaatgg
     8701 ttataagaca agggagcgat aattcatcgc tccctttttc gtgctcgctg tcgtattgac
     8761 ttaaccggga taagtacgag aatgagcgca catctgttta ccggaaacca gcatatatgg
     8821 atatctgctc ccgaaacgag aaattagcga ttagaagacc agcgatactg gttgcactgg
     8881 cacttttact gtgtagttgt aaaagcacgc ctccagagtc catggtgaca ccaccagcag
     8941 gttcaaagcc accagccacg acgcaacaat cgtcacaacc gatgcgtggc atctggctgg
     9001 ccacggtgtc tcgcctcgac tggccaccgg tttcctcggt taacattagt aaccccacca
     9061 gccgggcccg tgtacaacaa caggcgatga tcgacaaact ggatcatctg caacgtctcg
     9121 gtataaacac ggtctttttc caggttaagc cggacggtac cgccctgtgg ccatcgaaaa
     9181 ttttgccatg gtccgatctt atgaccggta agattggtga aaatccgggt tacgatccgc
     9241 tgcaatttat gctcgacgaa gcccacaagc gtgggatgaa agtacacgcc tggtttaacc
     9301 cctatcgcgt atcggttaat acgaagcccg gtactatcag ggaactgaat agcactctgt
     9361 ctcaacaacc ggcgagcgtc tatgtgcaac accgtgactg gatcagaacg tctggcgatc
     9421 gctttgtcct cgacccgggc atccctgagg ttcaggactg gatcacatca atagtcgcag
     9481 aagtggtttc ccgctatccg gtagatggcg tgcagtttga cgactatttc tatactgagt
     9541 caccgggttc acggctaaat gataacgaaa cgtaccgcaa atacggaggc gcatttgcgt
     9601 caaaagcaga ctggcggcgc aacaatactc agcagttaat tgcaaaagta tcgcacacca
     9661 ttaaaagcat taagccggga gtcgaatttg gcgttagccc ggcaggcgtg tggcgtaacc
     9721 gatcacacga tccgctcggt tccgataccc gaggcgcggc agcctatgac gaatcctacg
     9781 ctgacacccg tcgatgggtg gaacaaggat tgctggatta cattgctccc caaatttact
     9841 ggccgttctc acggagtgcc gcgcgttatg acgtgttggc aaaatggtgg gcggatgtcg
     9901 ttaaaccgac taggacccgc ctgtatatcg gtatcgcctt ctataaagtg ggtgaacctt
     9961 caaagataga gccagactgg atgattaacg gcggcgtacc ggaactgaaa aagcagctcg
    10021 atcttaacga tgcggtacca gaaattagcg gcaccatctt gttccgtgag gactatctga
    10081 ataaaccgca gactcaacaa gcggtcagct atctgcaaag tcgttggggc agttaaactc
    10141 ccatgcttac tttgtaacaa gccggtgctt tacccaccgg cttgttacac cttgtgaaat
    10201 atcacactag taatcatgct tacataagtc aaattaacca cgcaattaac cacacttagt
    10261 taaacagcta taaatcccta ataaaaatgt atgaatatga aaaaccatac gatattcgct
    10321 gtcaccgaaa aataattacg atgggctata ctcttctgaa gaacagtagc gtagattgaa
    10381 actgtcctaa gtcgcttatt tcttttcagt atatcttcat atttcaggag aatatggtgc
    10441 agttagtcaa tctcgcacgt tgcgttttga ttccgtctga tttcccagcc ttataagggt
    10501 gtgaattatg gagatgtatt ttaaaagaat gaaagatgag tggaccggac ttgtcgaaca
    10561 ggcagatcca ctcattcgtg ctaaagccgc ggaaattgcc gttgcgcatg ctcattatct
    10621 gagtatcgag ttttatcgaa ttgtccgcat cgacccgcat gccgaagaat tcttgagtaa
    10681 tgaacaagtt gagcggcagt tgaaaagtgc gatggaacgc tggattatta acgtgctttc
    10741 tgcccaggtt gacgatgtcg aaaggctaat acaaatccag cataccgtcg cggaagtgca
    10801 tgcccgcata ggaattccgg tagaaattgt cgagatgggg tttcgggtgc tgaaaaaaat
    10861 cctctatccg gtaatcttct cttcggatta ttccgccgca gaaaaacttc aggtctatca
    10921 tttctcgatt aacagtattg atatcgcgat ggaagtgatg acccgcgcgt ttacctttag
    10981 tgacagtagt gcctcaaagg aagatgaaaa ttatcgtatc ttctcgttac tggaaaacgc
    11041 cgaagaagaa aaagaacggc aaatagcctc aatactttca tgggaaatag atattatcta
    11101 taaaatcctg ctggattctg atttaggcag tagtttgcca ttaagccagg ctgattttgg
    11161 cctgtggttt aaccataaag gtcgacatta ttttagtggc attgccgaag taggccatat
    11221 ctcccgtctc attcaggatt tcgacggtat tttcaatcaa accatgcgta acaccaggaa
    11281 tttgaataac agaagtctgc gggtgaaatt tttattacag ataagaaata ccgtatcgca
    11341 gattattacc ttgctgcgtg aattgtttga agaagtatcg cgccacgaag tcggtatgga
    11401 tgtactaacg aaattactta accgccgttt cctgccgact atcttcaaac gcgaaattgc
    11461 ccatgccaac cggaccggta caccgctgtc agtgctgatt attgacgttg ataaattcaa
    11521 agagatcaac gatacgtggg gccataacac tggcgatgaa attctgcgta aagtctctca
    11581 ggccttttat gacaacgtcc acagtagtga ttatgttttt cgctacgggg gcgatgaatt
    11641 tatcattgtt ttgactgaag cttctgaaaa cgaaacgtta cgtaccgcag aacgtattcg
    11701 cagtcgggtg gagaaaacca aactgaaagc cgcaaacggc gaggatattg ccctctcact
    11761 ttccatcggt gccgccatgt ttaatggtca tcctgactat gagcgcctca ttcaaatagc
    11821 cgatgaagct ctgtatatcg ccaaaagacg aggtagaaac cgtgttgaac tctggaaagc
    11881 cagtctttag atgcgccagg atgcagaggt aatcatgaag ctaaccgatg cggaaaatgc
    11941 cgccgatggc atttttttcc ccgcccttga acaaaatatg atgggcgcgg tgttaattaa
    12001 cgaaaatgat gaagtgatgt ttttcaaccc cgccgcagag aagctctggg ggtacaaacg
    12061 tgaagaagtc attggcaata acattgatat gctgattccg cgggatttgc gtcctgcgca
    12121 tcctgaatac attcgtcata accgtgaagg tggtaaagcg cgcgtcgagg ggatgagtcg
    12181 ggagctgcag ctggagaaaa aagacggcag taaaatctgg acccgttttg cgctatcgaa
    12241 agtgagcgcc gaggggaaag tttattacct ggcgctggta cgggatgcca gcgtagaaat
    12301 ggcgcaaaaa gaacagaccc gacaattgat tattgccgtt gaccatctcg accgaccggt
    12361 gattgtcctc gatccggaac gccatattgt gcagtgcaat cgcgcattta ccgaaatgtt
    12421 tggttactgc attagcgaag ccagcggtat gcagcccgat acactcctga acattcctga
    12481 attccctgcc gataaccgca ttcgtttaca acagttgcta tggaaaaccg cccgcgatca
    12541 ggacgaattt ctgctgttga cacgcaccgg tgaaaaaatc tggattaaag cctctatcag
    12601 cccggtttat gacgtgctcg cgcatctgca gaacctggta atgaccttct cggatatcac
    12661 cgaagaacgg cagatccgcc agcttgaagg caatattctc gccgccatgt gcagcagccc
    12721 gccatttcat gaaatggggg aaatcatttg tcgtaacatc gaatctgttc tcaacgaatc
    12781 gcatgtttcg ctgttcgcac tgcgcaacgg gatgccgata cactgggcgt catcttcgca
    12841 cggtgcagaa gttcaaaatg cgcaaagctg gtcagcgacc attcgtcagc gtgatggtgc
    12901 gcctgcgggg atcctgcaaa ttaaaacctc gtcaggagca gaaaccagcg cctttatcga
    12961 acgcgtggca gatatcagcc agcatatggc cgcgctggcg ctggaacagg aaaaaagccg
    13021 tcagcatatt gaacaactca tccaatttga tccgatgacc ggtctgccaa atcgcaataa
    13081 cctgcacaat tacctcgatg accttgtcga caaagccgtc tctccggtgg tgtatctcat
    13141 cggtgttgac catattcagg atgtgattga tagccttggc tatgcgtggg ccgatcaggc
    13201 attgctggaa gtggtcaatc gctttcgtga aaaactcaaa ccggatcagt atctctgtcg
    13261 tatcgaaggt acgcagtttg tcctcgtgag cctggaaaac gacgtcagta acattaccca
    13321 aatcgccgat gagctacgga atgtggtcag caagccgata atgattgacg ataaaccatt
    13381 cccgctaacc ttgagtattg gcatcagcta cgacgtgggt aaaaaccgcg attacctgct
    13441 ctccactgct cacaatgcaa tggattatat tcgcaagaat ggcggtaacg gctggcagtt
    13501 cttcagcccg gcgatgaacg aaatggtaaa agagcgtttg gttttaggcg cagcgctgaa
    13561 agaagcgatt agcaataacc aactgaaact ggtttaccag ccgcaaatct tcgcagaaac
    13621 gggtgaactg tacggcatcg aagcccttgc tcgctggcac gatccccagc atggtcatgt
    13681 acccccttca cggtttattc ctctcgcaga agagattggt gaaatcgaaa atattgggcg
    13741 ctgggtcatc gcggaagctt gccgtcagtt agcagaatgg cgtagccaga atattcatat
    13801 cccggcgtta tccgtgaact tgtcggcgct gcactttcgc agtaatcagc tgcctaatca
    13861 ggtgtctgat gcaatgcaag cctggggtat tgacggccac cagctgacgg tagaaatcac
    13921 ggaaagcatg atgatggaac acgataccga aatctttaag cgcattcaga tcctgcgcga
    13981 tatgggcgtg ggcttatcgg tagatgattt tggcacgggc ttttccggat tatcccgctt
    14041 agtcagtctt ccggtaacgg aaatcaaaat tgacaaaagt tttgtcgatc gttgtctgac
    14101 ggaaaaacgc atccttgcct tacttgaagc cattaccagc attgggcaaa gcctcaattt
    14161 aaccgtcgtg gcggaaggcg tcgaaaccaa agagcaattt gagatgctac gcaagatcca
    14221 ctgtcgcgtt attcagggat atttcttttc ccgcccccta cccgccgagg aaattccagg
    14281 ctggatgagc agcgtgttac cgctgaaaat ctgacaaatt cctctcgccc gcactcgcgg
    14341 gttttcattt aacgtgacgc tgtcacttaa atactgtgat ttcagctacg attccgggag
    14401 attccttcct taacttcccc tcttgcacca aaactgcact acgctgcaca aatgttgatc
    14461 aaaagttaaa aaatataact ttaagccagc attctggcgc ttatcccggc atggcatgag
    14521 atctgcataa gcggaaagcg cagcaatttt tgtcttaata caggaaccgt ttatgtcgga
    14581 taccaccgaa ctggttgatt tagccgtgat cttccctgat ctggagatcg aattgaaata
    14641 cgcctgcgct gataacatca caggtaaagc tatttaccag caagcgcgtt gtctgttaca
    14701 caaggatgcg attaccgcgc tggcgaaaag tatcagtatc gcccagctgt caggcttaca
    14761 actggtgatt tacgatgcgt atcgcccaca acaagcacag gcgatgttgt ggcaagcctg
    14821 cccagacccg caatatgttg ttgatgtgac ggtcggttct aatcacagcc gtggcacggc
    14881 gatcgacctg acgcttcgtg atgagcacgg gaacatcctg gatatgggcg cagggttcga
    14941 tgaaatgcac gagcgctccc atgcctatca cccttccgtc ccgcccgccg ctcagcgcaa
    15001 tcgcctgttg ctgaatgcga taatgactgg tggcggtttt gtcggtatct ccagcgaatg
    15061 gtggcacttc gaattacccc aggcagcgag ttaccctctg ctcgctgatc aattctcttg
    15121 ttttatatcc cctggcacac agcacgtcag ttaatcagga aactgtcatg aagagatcga
    15181 tatcgtttcg tcccacattg ctcgcgctcg tccttgccac aactttcccg gttgcgcacg
    15241 ccgccgtacc gaaagatatg ctggtgattg gtaaggccgc cgatccacaa accctcgacc
    15301 cggcggtaac aatagataat aacgactgga cagtgaccta cccgtcttat cagcggctgg
    15361 ttcagtacaa aacggacggt gataaaggct caaccgacgt tgaaggcgat ctggcaagta
    15421 gctggaaagc gtctgacgat caaaaagagt ggacgttcac cctgaaggac aatgctaaat
    15481 ttgccgatgg cacacctgtc actgccgaag cagtaaaact ctcttttgag cggttactaa
    15541 aaatcggcca ggggccagca gaagcatttc ccaaagattt aaagattgat actcccgacg
    15601 aacatacggt gaagtttacc cttagccaac cattcgcacc gttcctctac acgctagcga
    15661 atgacggtgc atccattatc aatccggcgg tcttaaagga acatgcagcg gatgatgctc
    15721 gcggcttcct cgcgcaaaat accgccggtt ccggaccatt tatgctgaaa agcgggcaaa
    15781 aaggtcagca attagttctg gtgccaaatc cgcattaccc cggcaataaa ccgaacttta
    15841 agcgagtatc ggtaaaaatt atcggtgaaa gtgcctcccg tcgcctgcag ctctcccgtg
    15901 gcgacattga cattgccgat gcgctgccgg tggatcaact caacgccctg aagcaggaaa
    15961 ataaagtcaa tgtggcagag tatccgtcac tgcgcgttac ctatctgtat ctgaataaca
    16021 gcaaagcgcc tcttaatcag gcggatctgc gtcgggccat ttcctggtct accgattatc
    16081 agggcatggt taacggcatt ctgagtggta acggaaaaca gatgcgcggc ccgattccgg
    16141 aaggcatgtg gggctacgat gcgacggcaa tgcaatacaa ccatgacgaa acgaaagcca
    16201 aagccgaatg ggataaagtg acgagcaaac ccaccagcct gacgtttctc tactccgata
    16261 acgatccgaa ctgggagcct attgctctgg cgacacaatc cagtctcaac aagctgggca
    16321 tcaatgtgaa gctggaaaag ctggcgaacg ccaccatgcg cgacagagtg ggtaaaggtg
    16381 attacgacat tgcgattggc aactggagtc cggattttgc cgacccgtat atgtttatga
    16441 attactggtt tgagtcagac aaaaaaggtc tgccgggtaa ccgctcgttc tatgaaaaca
    16501 gtgaggtcga taagttactg cgcaatgcgc ttgcgaccac cgaccagacg cagcgtaccc
    16561 gggactacca gcaggcacag aaaatcgtca ttgatgacgc tgcttatgtg tacctgttcc
    16621 agaaaaacta ccaactggcg atgaacaaag aggtgaaagg ctttgtgttc aatcccatgc
    16681 tggaacaggt cttcaatatc aataccatga gtaaataacg tgaccttctg gagtatttta
    16741 cgccaacgct gctgggggct ggtgctcgtg gtggcgggcg tctgcgtgat tacgtttatt
    16801 atctcgcatc tgatccctgg cgatccggcg cggttactgg cgggtgaccg cgccagcgat
    16861 gctatcgtgg aaaatattcg ccagcaactg ggactggacc agccactgta tgtacagttt
    16921 taccgctact tcagcgacct gtttcatggt gacctgggaa catccattcg taccgggcgt
    16981 ccggtgctgg aagagttgcg tatatttttc ccggcgacgc tggaactggc tttttgtgcc
    17041 ctgctgctgg cactcctgat tggcatcccg ctgggcatac tctctgcagt ctggcgaaat
    17101 cgctggctgg atcatctggt gcgaataatg gccattaccg gaatctccac acctgcgttc
    17161 tggcttggac tgggcgtcat tgtgctgttt tatggtcatc tgcaaattct tcccggcggc
    17221 ggaaggcttg atgactggct ggatccacca acgcacgtta ccggctttta tctgctcgat
    17281 gcgctgcttg aaggcaacgg tgaagtcttc ttcaatgcgt tgcaacatct catcttaccg
    17341 gcattaacgc tggcgttcgt tcacctggga attgtcgctc gccagatccg ctcagcgatg
    17401 ctggaacaat tgagtgaaga ctacattcgt accgcccggg ccagcggctt gcccggctgg
    17461 tatatcgttt tatgttatgc gctacccaat gcgttgatcc catcgattac cgtattgggt
    17521 ctggcgctgg gcgatttgtt gtatggcgca gtgctcaccg aaaccgtttt tgcctggccc
    17581 ggaatgggtg catgggtagt aacatcaata caggcgctcg acttcccggc agtgatgggc
    17641 tttgccgtcg tggtttcatt tgcttatgtg ctggtcaacc tggtggtgga tttgctctat
    17701 ttgtggattg atccgcgtat cggacgtgga ggtggtgaat gatgctaagc gaggaaacgt
    17761 ccgccgtacg cccacaaaaa caaacgcgat ttaacggtgc aaaactggta tggatgctga
    17821 aaggcagtcc gctcaccgtg accggcgcag tcatcattgt attaatgcta ttgatgatga
    17881 ttttttcacc gtggcttgcg acgcatgatc ccaacgccat tgatttaacc gcccgccttt
    17941 tgccgccttc tgcggcgcac tggtttggca ccgatgaagt gggacgcgat ctgttcagcc
    18001 gcgtactggt cggcagtcag caatcaattc tcgccggatt agtcgtggtt gccattgcgg
    18061 gtatgattgg ttcgctactc ggatgtctat ccggtgtgct tggcggacgc gcagacgcca
    18121 tcatcatgcg catcatggac attatgctgt cgattccttc gttggtactg acaatggcac
    18181 tggctgccgc tctcgggccg agtttattta acgccatgct ggcgattgct attgtgcgaa
    18241 ttccctttta tgtgcgcctg gcgcgggggc aagcattagt tgtacgccag tatacctatg
    18301 ttcaggcggc gaaaaccttt ggtgcgtcgc gttggcatct gatcagctgg catattttac
    18361 gtaactccct gccgccgctg atcgtgccgg catcgctgga tatcggtagc gcgattttaa
    18421 tggccgccac gttgggattt attggcctgg gtgctcaaca accgagtgct gaatggggcg
    18481 cgatggtagc taatggtcgc aactatgtac tcgatcagtg gtggtattgc gcatttccgg
    18541 gggcagcgat tttgcttacc gccgtcgggt ttaatctctt tggtgatggt attcgcgatc
    18601 tgcttgaccc gaaagcagga ggaaagcagt catgacccaa cccgttctgg acattcaaca
    18661 gctgcatttg agtttccccg gttttaacgg ttatgttcac gtgctcaaca atgtgtcctt
    18721 gcagattaac cgcggtgaaa ttgtcggtct ggtgggagaa tccggctcag gtaaatcagt
    18781 caccgcaatg ctgattatgc gtctgctacc gacgggcagt tattgcgtac atcgggggca
    18841 gatttcactg ctgggagacg atgttcttaa cgctcgggag aaacagcttc gtcagtggcg
    18901 cggcgcacga gtggcgatga tctttcagga accgatgacc gccctcaatc cgacacgtcg
    18961 aataggtctt cagatgatgg acgtgatccg ccatcatcaa ccaataagtc gtcgggaagc
    19021 cagagctaaa gcgattaccc tgctggaaga gatgcaaatc ccggatgccg tggaagttat
    19081 gtcgcgctat ccgtttgagc tttcaggtgg tatgcgccag cgggtaatga ttgcgctggc
    19141 attctcctgc gagccgcaat tgattattgc cgacgaaccg actacggcgc tggacgtcac
    19201 ggtacagttg caggtactgc gtttgcttaa acataaagct cgcgccagtg gaactgcggt
    19261 actgttcatc agccatgata tggccgtggt gtcgcaactg tgcgatagcg tttacgtgat
    19321 gtatgccgga agcgtaattg aaagcggcgt gacggcagac gttatccatc atccccggca
    19381 tccgtatacc attggtttgc tgcaatgcgc accagaacat ggagtaccac gccagccatt
    19441 acccgccatt ccagggacgg taccaaacct cacccatttg cctgacggct gcgcttttcg
    19501 cgatcgttgc tatgcggcag gtgcacagtg tgaaaacgtc ccggcgctga cagcgtgtgg
    19561 tgacaacaac cagcgctgcg cctgttggta tccgcagcag gaggtcataa gtatctgaca
    19621 cgttattaac gttacgcgac gtccatatca atttcccggc ccgtaaaagc tggcttggta
    19681 aaactacgga acatgttcat gcgattaatg gtattgattt acagatccgc cgtggtgaaa
    19741 ccttagggat cgtcggtgag tcaggctgcg gcaaaagcac cctcgcacag cttttaatgg
    19801 gtatgctgca accgagccac gggcaataca tccgttcagg atcacaacgc attatgcaga
    19861 tggtgtttca ggacccgctc tcttcgctta atccgcgctt atcggtgtgg cgcatcatca
    19921 cagaaccgct ctggatagct aagcgtagta gtgaacaaca gcggcgagcg ttggcaaagg
    19981 agttggctgt gcaggtgggt attcgtccgg agtatctcga ccgcctgcct catgcgttct
    20041 ccggcgggca gcggcaacgc atcgccattg ccagagcact ctcttcgcag cctgacgtga
    20101 ttgtgcttga tgagccaacc tcggcgctgg atatctccgt gcaggcgcag atcctcaatt
    20161 tactggtaac gctacaggaa aatcacgggc tgacttatgt gctgatttca cacaatgtct
    20221 cggtgatacg tcatatgagc gatcgggtgg cggtgatgta tctcgggcag attgtagaac
    20281 tgggcgacgc gcagcaggtg ctgacggcac ctgcacatcc atacacccga ttattgctgg
    20341 attccctccc cgccattgat aaaccgctgg aggaagaatg ggcattatgt aaaacggatc
    20401 tgccgggaaa ccgcacgttg ccacaaggct gttttttcta cgaacgttgc cctctggcaa
    20461 cccacggatg tgaagtccgg caatcattag caataaggga ggacggacgt gagctccggt
    20521 gctggcgggc gctgtagaga tgctaacaac gcatcaggca ttcacgcgcc tgacgcgtca
    20581 tccggcaatg ctttacgatt tcaactggta atccagcgta atttccgctt tcagtacctg
    20641 agaaaccggg catcctgctt ttgctttctg gatgatgccg tcaaaggtag aggcatcaat
    20701 acccggcacc gcaacttcac tcttcagtgc gattttcgta atcgcaaaat cggcatccac
    20761 tttatccagc gacacatcgg cggtggtatc aatcgatgtt ggcgtgaatc ccgcttcccc
    20821 cagcattaat gaaagcgcca ttgagaaaca tgcggcatgc gctgcgccaa tcagttcttc
    20881 agggttggtt cctttttcgc cttcaaaacg cgtgttaaat ccatacggct gttggttcag
    20941 cacgccactc tgggtggata ctgttccctt cccgcgtttg atatcgcctt cccagtgtgc
    21001 ctgacctttc ttatggattg tcattgttgc tctcctgtgg gcttaaaaag gaaattatag
    21061 actacgtgag aaacagcacg aggataagca ggatattccg aataaaatac ggcgaaaata
    21121 ttacttctgc acttaaatta aattcccaca atagataacc gggaatatat atctcaagag
    21181 atattgcatc aatatcaatc aactcaccac aataataata catatcaata tcatggagtt
    21241 aaaatataaa aatcatcaac caggactaat cttaacaacg aagcggcaaa tatttgccat
    21301 gctgaatgtg ctttataaat ctgcgatccg tagcagacac cataaataca cagacacgga
    21361 gaatcactat gtttacttat tatcaggcag aaaat
//
LOCUS       DADMKF010000063        21159 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.064, whole genome shotgun sequence.
ACCESSION   DADMKF010000063 DADMKF010000000
VERSION     DADMKF010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21159
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.064"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            <1..384
                     /locus_tag="JNC24_002150"
     CDS             <1..384
                     /locus_tag="JNC24_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HAY9158891.1"
                     /translation="VQSGSSVMFIAIDMDKLKQINDTLGHQEGDLAITLLAQAIKQSI
                     RKSDYAIRLGGDEFCIILVDSTPQIAAQLPERIEKRLQHIAPQKEIGFSSGIYAMKEN
                     DTLHDAYKASDERLYVNKQNKNSRS"
     gene            427..930
                     /gene="yeaK"
                     /locus_tag="JNC24_002151"
     CDS             427..930
                     /gene="yeaK"
                     /locus_tag="JNC24_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HAY9158892.1"
                     /translation="MTEMAKGSVTHQRLIALLSQEGANFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTTDYLRIAQPEL
                     LNFRRTA"
     gene            complement(931..1035)
                     /locus_tag="JNC24_002152"
     CDS             complement(931..1035)
                     /locus_tag="JNC24_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9158893.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERTG"
     gene            1205..1651
                     /locus_tag="JNC24_002153"
     CDS             1205..1651
                     /locus_tag="JNC24_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HAY9158894.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            complement(1608..2429)
                     /locus_tag="JNC24_002154"
     CDS             complement(1608..2429)
                     /locus_tag="JNC24_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY9158895.1"
                     /translation="MMHRLNLNGYEPDRHHEAAVAFCIHAGTDELTSPVHQHRKGQLI
                     LALHGAITCTVENALWMVPPQYAVWIPGGVEHSNQVTANAELCFLFIEPSAVTMPTTC
                     CTLKISPLCRELILTLANRTTTQRAEPMTRRLIQVLFDELPQQPQQQLHLPVSSHPKI
                     RTMVEMMAKGPVEWGSLGQWAGFFAMSERNLARLIVKETGLSFRQWRQQLQLIMALQG
                     LVKGDTVQKVAHTLGYDSTTAFITMFKKGLGQTPGRYIAGLTTVSPQSAKPDPRQ"
     gene            2526..3707
                     /gene="nimT"
                     /locus_tag="JNC24_002155"
     CDS             2526..3707
                     /gene="nimT"
                     /locus_tag="JNC24_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
                     /protein_id="HAY9158896.1"
                     /translation="MTCSTSLSGKNRIVLIAGILMIATTLRVTFIGAAPLLDTIRSAY
                     SLTTAQTGLLTTLPLLAFALISPLATPVARRFGMERSLFAALLLICAGIAIRSLPSPY
                     LLFGGTAVIGGGIALGNVLLPGLIKRDFPHSVARLTGAYSLTMGAAAALGSAMIVPLA
                     LNGFGWQGALLMLMCFPLLALFLWLPQWRSQQHANLSTSRALHTRGIWRSPLAWQVTL
                     FLGINSLIYYVIIGWLPAILISHGYSEAQAGSLHGLLQLATAAPGLLIPLFLHHVKDQ
                     RGIAAFVALMCAVGAVGLCFMPAHAITWTLLFGFGSGATMILGLTFIGLRASSAHQAA
                     ALSGMAQSVGYLLAACGPPLMGKIHDANGDWSVPLMGVAILSLLMAIFGLCAGRDKEI
                     R"
     gene            3762..4109
                     /locus_tag="JNC24_002156"
     CDS             3762..4109
                     /locus_tag="JNC24_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HAY9158897.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYLAELAQHEQEGKRLADIAKKQPLTLLYAAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(4131..4385)
                     /gene="yoaF"
                     /locus_tag="JNC24_002157"
     CDS             complement(4131..4385)
                     /gene="yoaF"
                     /locus_tag="JNC24_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HAY9158898.1"
                     /translation="MKIISFALPCLLVLAGCSTSSQPEVPKPPQIGMANPASVYCQQK
                     GGTLIPVSTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            4568..5593
                     /gene="dgcP"
                     /locus_tag="JNC24_002158"
     CDS             4568..5593
                     /gene="dgcP"
                     /locus_tag="JNC24_002158"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HAY9158899.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVSWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGISTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLREANAALIAQSYTDSLTGLPNPRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     AHSAPHC"
     gene            5626..5724
                     /gene="yoaK"
                     /locus_tag="JNC24_002159"
     CDS             5626..5724
                     /gene="yoaK"
                     /locus_tag="JNC24_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HAY9158900.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGGYAAPGA"
     gene            5727..5801
                     /gene="yoaJ"
                     /locus_tag="JNC24_002160"
     CDS             5727..5801
                     /gene="yoaJ"
                     /locus_tag="JNC24_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HAY9158901.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(5860..6108)
                     /locus_tag="JNC24_002161"
     CDS             complement(5860..6108)
                     /locus_tag="JNC24_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAY9158902.1"
                     /translation="MGILSWIIFGLIAGILAKWIMPGKDGGGFFMTILLGIVGAVVGG
                     WISTLFGFGKVDGFNFGSFVVAVIGAIVVLFIYRKIKS"
     gene            complement(6256..6438)
                     /locus_tag="JNC24_002162"
     CDS             complement(6256..6438)
                     /locus_tag="JNC24_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1869 domain-containing protein"
                     /protein_id="HAY9158903.1"
                     /translation="MGKATYTVTVTNNSNGVSVDYETETPMTLLVPEVAAEVIKDLVN
                     TVRSYDTENEHDVCGW"
     gene            complement(6442..6801)
                     /gene="yeaR"
                     /locus_tag="JNC24_002163"
     CDS             complement(6442..6801)
                     /gene="yeaR"
                     /locus_tag="JNC24_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1971 domain-containing protein"
                     /protein_id="HAY9158904.1"
                     /translation="MLQIPQNYIHTRSTPFWNKQTAPAGIFERHLDKGTRPGVYPRLS
                     VMHGAVKYLGYADEHSAEPDQVILIEAGQFEVFPPEKWHNIEAMTDDTYFNIDFFVAP
                     EVLMEGAQQRKVIHNGK"
     gene            complement(6974..7612)
                     /gene="leuE"
                     /locus_tag="JNC24_002164"
     CDS             complement(6974..7612)
                     /gene="leuE"
                     /locus_tag="JNC24_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine efflux protein LeuE"
                     /protein_id="HAY9158905.1"
                     /translation="MFAEYGVLNYWTYLVGAIFIVLVPGPNTLFVLKNSVSSGMKGGY
                     LAACGVFIGDAVLMFLAWAGVATLIKTTPILFNIVRYLGAFYLLYLGSKILYATLKGK
                     NNEAKSDEPQYGAIFKRALILSLTNPKAILFYVSFFVQFIDVNAPHTGISFFILATTL
                     ELVSFCYLSFLIISGAFVTQYIRTKKKLAKVGNSLIGLMFVGFAARLATLQS"
     gene            complement(7739..8662)
                     /locus_tag="JNC24_002165"
     CDS             complement(7739..8662)
                     /locus_tag="JNC24_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY9158906.1"
                     /translation="MNNLPLLNDLRVFMLVARRAGFAAVAEELGVSPAFVSKRIALLE
                     QTLNVVLLHRTTRRVTITEEGERIYEWAQRILQDVGQMMDELSDVRQVPQGMLRIISS
                     FGFGRQVVAPALSALAKAYPQLELRFDVEDRLVDLVNEGVDLDIRIGDDIAPNLIARK
                     LATNYRILCASPEFIAQHGAPKHLTDLSALPCLVIKERDHPFGVWQLRNKEGHHAIKV
                     TGPLSSNHGEIVHQWCLDGQGIALRSWWDVSENIASGHLVQVLPEYYQPANVWAVYVS
                     RLATSAKVRITIEFLRQYFAEHYPNFSLEHA"
     gene            8765..9850
                     /gene="dmlA"
                     /locus_tag="JNC24_002166"
     CDS             8765..9850
                     /gene="dmlA"
                     /locus_tag="JNC24_002166"
                     /EC_number="1.1.1.83"
                     /EC_number="1.1.1.85"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional
                     D-malate/3-isopropylmalate/tartarate dehydrogenase"
                     /protein_id="HAY9158907.1"
                     /translation="MMKTMRIAAIPGDGIGKEVLPEGIRVLQAAAERWGFDLSFEQME
                     WASCEYYSHHGKMMPDDWHEQLSRFDAIYFGAVGWPDTVPDHISLWGSLLKFRREFDQ
                     YVNLRPVRLFPGVPCPLAGKQPGDIDFYVVRENTEGEYSSLGGRVNEGTEHEVVIQES
                     VFTRRGVDRILRYAFELAQSRPRKTLTSATKSNGLAISMPYWDERVEAMAENYPEIRW
                     DKQHIDILCARFVMQPERFDVVVASNLFGDILSDLGPACTGTIGIAPSANLNPERTFP
                     SLFEPVHGSAPDIYGKNIANPIATIWAGAMMLDFLGNGDERFQQAHNGILAAIEEVIA
                     HGPKTPDMKGSATTPQVADAICKIILR"
     gene            10101..11711
                     /gene="yeaV"
                     /locus_tag="JNC24_002167"
     CDS             10101..11711
                     /gene="yeaV"
                     /locus_tag="JNC24_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter YeaV"
                     /protein_id="HAY9158908.1"
                     /translation="MMSNVKKKDVPLISISLVAILFIAAALSLFPQQSADAANTIYTF
                     VTRTLGSAVQVLVLLAMGLVIYLATSKYGNIRLGEGKPEYSTLSWLFMFICAGLGSST
                     LYWGVAEWAYYYQTPGLNIAPRSQQALEFSVPYSFFHWGISTWATYTLASLIMAYHFH
                     VRKNKGLSLSGIIAAITGVRPQGPWGKLVDLMFLIATVGALTISLVVTAATFTRGLSA
                     LTGLPDNFTVQAFVILLSGGIFCLSSWIGINNGLQRLSKMVGWGAFLLPLLVLIVGPT
                     EFITNSIINAIGLTTQNFLQMSLFTDPLGDGSFTRNWTVFYWLWWISYPPGVAMFVTR
                     VSRGRKIKEVIWGLILGSTVGCWFFFGVMESYAIHQFINGVINVPQVLETLGGETAVQ
                     QVLMSLPAGKLFLAAYLGVMIIFLASHMDAVAYTMAATSTRNLQEGDDPDRGLRLFWC
                     VVITLIPLSILFTGASLETMKTTVVLTALPFLVILLVKVGGFIRWLKQDYADIPAHQV
                     EHYLPQTPVEALEKTPVLPAGTVFKGDN"
     gene            11743..12867
                     /gene="yeaW"
                     /locus_tag="JNC24_002168"
     CDS             11743..12867
                     /gene="yeaW"
                     /locus_tag="JNC24_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaW"
                     /protein_id="HAY9158909.1"
                     /translation="MSNLSPDFVLPENFCANPQEAWTIPARFYTDQNAFEHEKENVFA
                     KSWICVAHSSELANANDYVTREIIGESIVLVRGRDKVLRAFYNVCPHRGHQLLSGEGK
                     AKNVITCPYHAWAFKLDGNLAHARNCENVANFDSDKAQLVPVRLEEYAGFVFINMDPN
                     ATSVEDQLPGLGAKVLEACPEVHDLKLAARFTTRTPANWKNIVDNYLECYHCGPAHPG
                     FSDSVQVDRYWHTMHGNWTLQYGFAKPSEQSFKFEEGTDAAFHGFWLWPCTMLNVTPI
                     KGMMTVIYEFPVDSETTLQNYDIYFTNEELTDEQKSLIEWYRDVFRPEDLRLVESVQK
                     GLKSRGYRGQGRIMADSSGSGISEHGIAHFHNLLAQVFKD"
     gene            12923..13888
                     /gene="yeaX"
                     /locus_tag="JNC24_002169"
     CDS             12923..13888
                     /gene="yeaX"
                     /locus_tag="JNC24_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaX"
                     /protein_id="HAY9158910.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHII
                     VQMSDGDNQYSNAYSLLSSPHDTSCYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIAGGIGITPFLSHMAELQHSDVDWQLHYCSRNPESCAFRDELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALNEAVRSEAARLDIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(13942..15057)
                     /gene="rnd"
                     /locus_tag="JNC24_002170"
     CDS             complement(13942..15057)
                     /gene="rnd"
                     /locus_tag="JNC24_002170"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HAY9158911.1"
                     /translation="MITTDDALASLCEAVRVFPAIAQDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEEALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(15139..16824)
                     /gene="fadD"
                     /locus_tag="JNC24_002171"
     CDS             complement(15139..16824)
                     /gene="fadD"
                     /locus_tag="JNC24_002171"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HAY9158912.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLIDMFEQSVVRYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGMIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCCRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            complement(17029..17610)
                     /gene="yeaY"
                     /locus_tag="JNC24_002172"
     CDS             complement(17029..17610)
                     /gene="yeaY"
                     /locus_tag="JNC24_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HAY9158913.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MNAPDLYVGQEARFGGKVVVVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGTVDGKVNNAPYKFMVMQVTGYKRWHITQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVETIVTE"
     gene            complement(17650..18345)
                     /gene="tsaB"
                     /locus_tag="JNC24_002173"
     CDS             complement(17650..18345)
                     /gene="tsaB"
                     /locus_tag="JNC24_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HAY9158914.1"
                     /translation="MRILAIDTATEVCSVALWNDGNVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLAAIDARMGEVYWAEYQRDENGIWRGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLRKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            complement(18403..20313)
                     /locus_tag="JNC24_002174"
     CDS             complement(18403..20313)
                     /locus_tag="JNC24_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HAY9158915.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGCAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDLLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDNRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            20445..20789
                     /locus_tag="JNC24_002175"
     CDS             20445..20789
                     /locus_tag="JNC24_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HAY9158916.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
BASE COUNT         5171 a         5446 c         5153 g         5389 t
ORIGIN      
        1 gtgcaatccg gttcttcggt gatgtttatt gcaattgaca tggacaagtt aaagcaaata
       61 aatgacaccc tcggtcatca ggagggggat ttagcgatta cgttattagc tcaggcgatt
      121 aaacaatcga ttcgtaaaag tgattatgcc atccgactcg gtggcgatga attctgcatc
      181 attcttgtcg attcgacgcc gcaaattgca gcacaactgc ctgaacgtat cgaaaaacgt
      241 ctgcaacata tcgcgccgca gaaagaaatc ggcttctctt ccggtattta cgcgatgaaa
      301 gaaaacgata cgttacatga tgcgtataaa gcttccgatg agcgtttata tgtcaataag
      361 cagaacaaaa acagccgttc atgataacct tctgtggttg tttgcttgta atctcaggag
      421 cgtgaaatga ctgaaatggc taaaggaagc gtgacgcatc agcggttaat cgctttatta
      481 tcacaagaag gtgctaactt ccgcgtcgtt acccatgaag cggtaggtaa atgtgaagcg
      541 gtgtcagaaa ttcgtggcac cgctttaggc cagggagcta aggcactggt atgtaaagtc
      601 aaaggcaatg gcgtaaatca acatgttctg gcgatcctcg ctgccgatca acaggccgat
      661 ctgagccaac ttgccagcca tataggcgga ttacgcgctt cgttagccag ccctgctgaa
      721 gtcgatgaac tcacaggctg tgtcttcggc gcgatccccc ctttcagctt ccatccaaaa
      781 ctcaaactgg ttgccgaccc tctactcttc gaacgatttg atgaaatcgc cttcaatgca
      841 gggatgctgg ataaatccgt tattttgaaa accacggatt atttgcgcat tgcgcaacca
      901 gaactgctca atttccgccg cactgcgtaa ctagcctgtc cgttcaataa gcagaacgga
      961 caaaaccagc acaacagcaa tggcaaaaaa cgatgacgtg ataatcagtg tctcgacgaa
     1021 catttgatcg ttcatagcat gcccccggga gatagctgtt ttcacgttat tgttagcgtg
     1081 cacaaatggc agtttgatga caattcgcct gattttttat gcaaaaaacg taaagatttt
     1141 ctacttcctt cctgcagcaa acgtaaagta agcaggctta ttattttttg gcaaggaaac
     1201 cacgatgttt gatgtcactt tgctgatcct gctcgggtta gctgcgctgg gctttatcag
     1261 tcataacacc actgtcgccg tttcaattct ggtgttgatc attgtccgcg tcacaccgtt
     1321 aagcaccttt ttcccgtgga ttgaaaaaca ggggctaagt atcgggataa tcattctgac
     1381 gattggcgtt atggccccca tcgccagcgg gacgctaccg ccctcaacct tgattcactc
     1441 gtttctgaac tggaaatcgc tggtggcaat tgcagtaggc gtgattgtct cctggctggg
     1501 tgggcgcggc gtgaccttaa tgggcagcca gccgcaactg gtcgccgggc tgctggtagg
     1561 cactgtttta ggcgtggcgc tgtttcgcgg cgtaccggtc ggcccgctta ttgccgcggg
     1621 tctggtttcg ctgattgtgg ggaaacagta gttaatcccg caatatatcg gcctggcgtt
     1681 tgccccaggc ctttcttaaa catagtaata aaggccgttg ttgaatcata ccccagcgta
     1741 tgagcaactt tctgtacagt atcgcctttc actaatccct gaagtgccat aatcagctgt
     1801 aactgctgac gccactggcg aaaactcaac cccgtctctt ttactatcag tcgcgcaaga
     1861 ttgcgttcac tcatcgcaaa aaagccagcc cattgcccta atgaccccca ctcgacgggc
     1921 cctttcgcca tcatctccac catcgtgcgg attttaggat gagaagaaac gggcaggtgc
     1981 agctgttgtt gtggctgctg agggagttca tcaaacagga cctgaattaa tctgcgggtc
     2041 atcggctcag cacgctgagt cgtcgtcctg tttgccaggg ttaaaatcaa ttcccggcat
     2101 aagggcgata ttttcaatgt gcaacatgtt gtcggcattg tcactgcgct gggttcgata
     2161 aacagaaaac agagttcagc gttagctgtc acttgattgc tatgttccac cccgcccggt
     2221 atccaaaccg catattgcgg cggcaccatc cataaagcat tttccaccgt acaggtaatt
     2281 gcaccatgta atgctagtat cagctgtcct ttgcggtgct gatgtacagg actggttaac
     2341 tcatccgttc cggcgtgaat acaaaatgcg accgcggctt catggtgtcg gtcgggttca
     2401 tagccattga gattcaacct gtgcatcatt ttgtccgaat ttagcgataa tttgtcattt
     2461 tagcttgatt caacataaca ataaaaacgg taaggtacag cctcgtttgt aacaatgaga
     2521 agcatatgac ctgttcaact tcattaagcg gcaaaaacag gattgtcctt atcgctggca
     2581 ttctgatgat tgccacaaca ttacgcgtca cctttatcgg cgcagcaccg ttactggata
     2641 cgattcgttc cgcttactcg ctgacgacag cgcaaaccgg cttattgacc accctgccat
     2701 tattggcctt tgcgctaatc tcacctttgg ccaccccggt agcgcgacgt tttggtatgg
     2761 aacgtagcct gtttgccgcg ttacttttga tctgtgctgg tatcgcaatt cgctctctcc
     2821 cttcgcctta cttattattt ggcggtacag cggtcattgg cggtgggatt gcattaggca
     2881 atgtcttact gccaggatta attaaacgcg atttccctca ttccgtcgcc agacttaccg
     2941 gcgcatattc cctgacaatg ggagctgcag cggcactggg atcggctatg atagtgccgc
     3001 tggctttgaa cggttttggc tggcaaggcg cgttgctcat gctgatgtgt tttcctctgc
     3061 tggctctttt tttatggctg ccacagtggc gaagccaaca acatgcaaat ttgagtacct
     3121 cgcgcgcctt acatactcgg ggtatctggc gttcgccgct tgcctggcag gtaacattgt
     3181 ttcttgggat caactcactg atctattacg tgattattgg ctggcttccg gcgatcctca
     3241 tcagtcacgg ctatagcgaa gcacaggcgg gttcactgca tggtttgctg caactagcca
     3301 cagcagctcc cggtttgctg atccctcttt tcttacatca tgtgaaagat cagcgtggta
     3361 ttgcagcgtt cgttgccttg atgtgcgcag tgggcgctgt tgggctctgc tttatgccag
     3421 cgcacgcgat cacctggact ctgcttttcg gttttggttc cggcgcaaca atgatactgg
     3481 ggttgacgtt cattggtctt cgggcaagtt ctgcgcatca ggcggcggca ctctcgggga
     3541 tggcacaatc cgtcgggtat ttgctggcag cctgtgggcc gccgctaatg ggtaaaatac
     3601 acgatgctaa cggtgactgg tccgtaccac tgatgggtgt tgcaatactt tcactactga
     3661 tggcgatttt cggactttgc gccgggagag acaaagaaat tcgctaatat ccggtgctat
     3721 agtgacgtga caaatcatgc gtgaaaggga gaacaaacac tatgaatatt cagtgcaaac
     3781 gcgtttatga tccggctgaa cagagcgatg gttatcgcgt actggtcgac cgcctctggc
     3841 cgcgcggtat caaaaaaacc gatttagccc ttgatgagtg ggataaagaa atcacgccgt
     3901 caacggaact gcgcaaagcc tttcacggcg aagtcgtcga ttttgcaacc tttcgcgagc
     3961 aatatcttgc agaactggcg caacacgagc aagaaggaaa gcggctggcg gacatcgcca
     4021 aaaaacagcc gctgaccctg ctctacgcag caaaaaacac cacgcagaac catgcgctgg
     4081 tgctggccga ctggctacgt agcttgtgat tttagtacag catccggcgg ttatttttca
     4141 ccagccggat ggtcccgtcg ccacaatgcc cattcatcaa tcgtttcacc gcccggtaat
     4201 ttgcaattgt tgctgacccc ttgcgctgtc gacactggaa tgagcgtccc gcccttctgc
     4261 tggcaataga ccgacgccgg atttgccata ccaatctgcg gcggtttagg tacttctggc
     4321 tgagaagagg ttgaacaacc agccaggacc agcaagcaag gcaaagcaaa actgataatt
     4381 ttcatttatt gatctcactc atttatctac gcttagagta tcgcggtatc gttttgtttt
     4441 gcagcactat ttttattaca ttcactcaaa acatattacg tcttgtttca tctttgttgg
     4501 tgatgtttta tcatgcctgc aaagattaaa taatcagcat ttacccgccg tatcctggag
     4561 ttgtttcgtg tcagatcaga ttatcgcccg cgtctcgcaa tcccttgcca aagaacagtc
     4621 actggaaagt ctggtccgac agcttctgga gatgctggaa atggtcactg atatggaatc
     4681 aacctacctg accaaagtgg atgtcgaagc gcgcctgcag catattatgt ttgcccgtaa
     4741 cagccagaaa atgcacatcc cggagaattt taccgtctcg tgggattact cgttatgcaa
     4801 acgcgccatt gatgaaaact gctttttcag cgatgaagtc cctgaccgtt ggggcgactg
     4861 tattgcggca cgcaatcttg gcatctccac atttctgagc acaccaattc acttaccgga
     4921 tggatcattc tatggcacgc tttgcgccgc cagcagtgag aagcgccagt ggagtgaacg
     4981 cgcggaacag gttttacagt tattcgccgg actgattgca caatatattc aaaaagaggc
     5041 actggttgaa cagttgcgcg aagccaatgc tgcgctgatt gcgcaatcgt ataccgactc
     5101 gttaaccggg ttgccgaatc cgcgggcgat ttttgaaaat ctgacgacgc tgttttctct
     5161 cgcccggcat cttaaccata agataatgat cgcgtttatc gatctggata acttcaaatt
     5221 aatcaatgat cgttttggtc ataatagtgg cgatctgttt ctcattcagg ttggcgagcg
     5281 ccttaatacg ctccagcaaa atggcgaagt tattggtcgt ctcggcggtg atgagttttt
     5341 ggttgtttca ctaaacaacg agaatgcgga tatttcgtcg ctgcgagaac gcattcagca
     5401 gcaaatacgt ggagaatatc acttaggtga tgttgatttg tattatcccg gtgccagtct
     5461 tggcatagta gaagtcgatc ctgaaacaac cgatgcagac agtgccctgc atgctgccga
     5521 tattgcgatg tatcaggaga aaaaacacaa acagaaaaca ccttttgtcg cgcattcagc
     5581 gccacattgc tgaggcgtat tcacatcctt ttgattggtg ataacatgcg aatcggtatt
     5641 atttttccgg ttgtaatctt tattacagcg gtcgtatttt tagcatggtt ttttattggc
     5701 ggctatgctg ccccgggggc ataaagatga aaaaaacaac gattattatg atgggtgtgg
     5761 cgattattgt cgtactcggc actgagctgg gatggtggta acgtcacctc taaaaaatag
     5821 caaaggctgc ctgtgtgcag cctttgtgca atttaagcgt taacttttaa tcttcctgta
     5881 gataaatagc acgacaatcg caccaataac ggcaaccacg aagctgccaa aattgaagcc
     5941 atcgacttta ccaaagccaa acagcgtgct gatccatccg ccgactacgg caccgactat
     6001 ccccagcagg atagtcataa agaatccacc accatcttta cctggcatga tccacttcgc
     6061 cagaataccg gcaataagcc caaaaataat ccatgacaga atgcccattg tttcctcact
     6121 tatctgtttt gcattagcgg gttagtcgct gataaaaagc atagcacaac atcgggaggg
     6181 caagatttgt gacgagcatc acggaggatt tttttgcgat ggcgcagaaa ttgcgccatc
     6241 aacgatcagt gataattacc aaccacaaac atcatgttcg ttttccgtgt cataagaacg
     6301 cacggtattc accagatctt ttatcacttc agccgccact tctggcacca gcaaagtcat
     6361 cggcgtctct gtttcataat cgacagaaac gccattactg ttattggtga cggtcacggt
     6421 atacgttgct ttgcccatga ttcatttccc gttatgaatg actttccgtt gttgcgcacc
     6481 ttccatcagg acttcaggag ccacgaagaa gtcaatgttg aaataagtat cgtcagtcat
     6541 ggcttcaatg ttgtgccact tttctggagg gaacacctca aactgccccg cttcgataag
     6601 gatcacctga tcaggctctg cactgtgttc atcagcgtag ccgagatatt tgaccgcccc
     6661 atgcataacg gaaaggcgtg ggtaaacccc cgggcgcgtt cctttatcaa gatgacgttc
     6721 gaatattccg gcaggcgcag tttgtttatt ccagaaaggc gttgagcgcg tatgaatata
     6781 attctgtggg atttgcagca tccttttccc tccttcggtg aatgcgctga aaacggttta
     6841 ttccagccgt ttcagggtac gcctgataat ttgcatttta aataccattt attggttact
     6901 ttttagcacc atatcagaga aaaatcaggg aggattacaa gtgggaagcc cgcgacaacg
     6961 cgggctgaat gtatcaggat tgcagcgtcg ccagtcgggc ggcgaaaccc acgaacatca
     7021 aaccaatcag tgagttgcca actttagcca gtttcttttt ggtacgtatg tactgcgtga
     7081 caaaagcacc agaaataatc aggaagctca aatagcagaa actcaccagt tccagcgtcg
     7141 tcgccagaat aaagaatgaa attcccgtat gtggggcatt aacatcgata aactgtacga
     7201 aaaacgacac atagaacaaa atggctttcg gattagtcag gctcaaaatt aacgcgcgtt
     7261 taaaaatggc cccgtattgg ggctcatcgg atttggcctc gttattttta cccttcaggg
     7321 tcgcgtagag aattttactc cccagataga gcaaataaaa cgcaccaaga taacgtacaa
     7381 tgttgaataa tatcggggtg gtcttaatta atgtcgccac tccagcccat gccagaaaca
     7441 tcaatactgc atcgccaata aatacaccgc aggccgcaag ataaccgcct ttcataccgc
     7501 tactgacgct atttttgagt acaaacaggg tatttggccc tggcaccaac acaataaaaa
     7561 tggccccaac cagataggtc cagtaattca gaaccccgta ttcagcgaac acattaacct
     7621 ctttaattat ctttcgatca tgcgcgatta aaggtgaata tgctaaccaa tctgtagcgg
     7681 cttagaaagg agaaaatcag gttttaaccc gatatcaacc cgataattga ataattaatc
     7741 aggcatgctc cagtgaaaaa ttcgggtagt gctcggcaaa atactggcgt aaaaattcta
     7801 tcgttatccg cactttcgct gacgtcgcca gccttgaaac ataaacggcc cagacgttcg
     7861 ctggctggta atattccggt agcacttgca ctaaatgacc actggcaatg ttttcgctaa
     7921 catcccacca ggagcgcaga gcaatcccct gcccatcgag acaccactga tggacaattt
     7981 ctccatgatt agacgacagt gggccggtga ctttaatcgc atggtggcct tctttattgc
     8041 gtaattgcca gacaccaaaa ggatgatcgc gctctttaat gaccaggcac ggcaatgcgg
     8101 aaagatcggt taaatgtttc ggcgcgccat gttgcgcaat aaattccggc gacgcgcaga
     8161 gaatgcggta attagtcgcc agtttgcggg cgattaaatt aggggcgatg tcatcgccaa
     8221 tgcgaatatc gagatcaaca ccttcattga ccaaatcgac caaccggtct tccacatcaa
     8281 aacgtaattc cagttgcgga tacgccttag ccagcgctga taacgccgga gccaccacct
     8341 gtcgtccaaa accaaagctg ctgataatac gcagcatccc ctgcggcacc tgacgcacgt
     8401 cagaaagttc gtccatcatc tgaccgacat cctgcaaaat ccgctgcgcc cattcataaa
     8461 tccgctctcc ttcttcggta atggtgacgc ggcgggtggt gcggtgtaac aacaccacgt
     8521 ttagcgtttg ctccagcaag gcgatgcgct tgctgacgaa cgccggtgaa acgcccagtt
     8581 cttccgccac ggcggcaaaa ccagcccggc gagccaccag cataaagacg cgcaaatcgt
     8641 tcagcagcgg taaattattc atgattcgtg ttttatgttt caccagttac ggggattaat
     8701 tccttttcag tcaattatag gatggtgatg ttgtcaattt tgatggtcag gaagtgagaa
     8761 cccaatgatg aaaacgatgc gtattgctgc gatcccggga gatgggattg gcaaagaagt
     8821 ccttcctgaa gggattcgcg tattacaggc tgccgctgag cgctggggct tcgacttgag
     8881 ttttgagcaa atggagtggg cgagctgcga gtattacagc catcacggta aaatgatgcc
     8941 ggacgactgg catgagcaac ttagccgttt cgacgccatc tattttggtg ccgtcggctg
     9001 gccggatacc gttccggacc atatttcgtt gtggggttcg ctgctgaaat ttcgtcgtga
     9061 attcgaccag tacgtcaacc tgcgcccggt tcgtctcttt cctggcgttc cctgcccgct
     9121 ggcgggaaaa cagcctggcg acatcgattt ttacgtggtc agggaaaaca ccgaaggcga
     9181 atattcctcg ctcggcggta gagtgaatga aggtacagag catgaagtcg tcattcagga
     9241 atcggtattt acgcgtcgtg gtgtcgatcg cattttgcgt tatgccttcg aacttgcgca
     9301 aagccgccca cgtaagacgc taacttctgc cactaaatca aacggtttag ccatcagcat
     9361 gccgtactgg gatgagcgag tggaagcaat ggccgagaat tacccggaga tccgctggga
     9421 caagcagcat attgatattc tctgcgcgcg ttttgtgatg cagccggaac gcttcgatgt
     9481 ggtggtggcg tccaatttgt ttggcgatat cctttccgat cttggcccgg cctgcaccgg
     9541 caccattggc attgccccat ccgccaacct gaatccggaa cgcactttcc cgtcgctctt
     9601 cgagcctgtc cacggttccg cgccggatat ctacgggaaa aatattgcta accctatcgc
     9661 cacaatttgg gccggtgcga tgatgctcga ttttctcggc aatggcgatg agcgtttcca
     9721 gcaagcgcat aacggtattc tggcagcgat tgaagaagtg attgctcacg ggccgaaaac
     9781 accggatatg aaaggcagtg ccaccacgcc acaggttgcc gacgcgattt gcaaaattat
     9841 tttgcgttaa ggtcaaacca gtttatttga accgcgtcac tgacgcggtt tttttattcg
     9901 ttctttgcag ttaataacct gcgtcatttc accttttatt gtttccgttt cgtgttttat
     9961 ggctttccgt attcttaatt gtttaattta tgtaacatgc aaataatcaa tttgttaaac
    10021 acaacataca actcattacc ctgcaaataa ttacaactgg caaaagccag agacgggctt
    10081 ttatgcccag gagatgactc atgatgagca atgttaagaa aaaagatgtg ccgctgataa
    10141 gcatcagcct ggtggccatt cttttcatcg cagctgcatt aagccttttc ccacaacaat
    10201 cggccgacgc ggccaacaca atatacactt ttgttacgcg tacgttaggt tccgccgtac
    10261 aggtattggt tttgctggca atgggactgg tgatttattt agccaccagt aaatacggca
    10321 atattcgtct tggcgaagga aaaccggaat acagcacgct ctcctggctg tttatgttta
    10381 tttgtgccgg tttaggttct tctacgcttt attggggggt tgctgaatgg gcctattatt
    10441 atcaaacgcc tggattaaat atcgcaccac gttcacaaca ggcactcgaa tttagcgtcc
    10501 cttactcctt cttccactgg ggcatcagca cctgggcaac ttatacgctg gcctcattaa
    10561 tcatggctta tcactttcat gtgcgtaaaa acaaaggtct gagtctttcc ggcattattg
    10621 ccgcgattac cggcgttcgc ccgcaaggcc catggggaaa actggttgat ttgatgtttc
    10681 tgatcgccac tgtcggcgca ctgactattt cccttgttgt caccgcagca acctttactc
    10741 gtggactttc cgcgctgacc ggtttacccg ataacttcac cgtgcaggca tttgtgatcc
    10801 tgctttccgg cggcattttt tgcctaagct cgtggattgg tatcaacaac ggtttgcaac
    10861 gtctgagcaa aatggttggc tggggcgcgt tcctgctgcc attactggtg ctgattgtcg
    10921 gtccaaccga atttattacc aacagcatca tcaatgccat cggcctgacc acgcaaaact
    10981 tcctgcaaat gagcttattc accgatccgc ttggcgatgg ttcatttacc cgcaactgga
    11041 ccgttttcta ctggctgtgg tggatctcat acccccctgg cgtagcaatg tttgtcaccc
    11101 gcgtttcccg cggtcgtaag attaaagaag ttatctgggg actgatcctc ggcagcaccg
    11161 tcggttgctg gttcttcttc ggcgtaatgg aaagctatgc cattcatcag tttatcaatg
    11221 gcgtaatcaa cgtcccacag gtgctggaaa cactgggcgg cgaaacagct gtacagcaag
    11281 ttctgatgtc gttgccagcc ggtaaattgt tcctcgccgc atacctgggc gtgatgatta
    11341 ttttccttgc ctcgcatatg gatgcagtgg cctacaccat ggcggcgacc agtacgcgta
    11401 atctccagga aggtgacgat cctgaccgtg ggctgcgtct tttctggtgc gtggtgatca
    11461 ctctgatccc gctttccatc ttgtttaccg gtgcttcgct ggaaacgatg aaaaccaccg
    11521 tcgtgctcac agcccttccc ttcctcgtca ttttactggt gaaagtcggc ggatttattc
    11581 gctggctgaa acaggattat gccgacattc cggctcatca agttgaacat tatctcccgc
    11641 agacaccggt tgaagccctg gaaaaaacac cagtgctccc tgcgggaacc gtattcaaag
    11701 gcgacaactg agcgccatca tcctaacgat aaaggtatcc ctatgagcaa tctgagccct
    11761 gactttgtac tacccgaaaa tttttgcgct aacccgcaag aggcgtggac cattcctgcc
    11821 cgtttttata ccgatcagaa cgcgtttgaa cacgaaaaag agaacgtctt cgccaaaagc
    11881 tggatttgcg tcgctcacag cagcgaactg gcgaatgcca atgattatgt gacgcgtgag
    11941 atcattggcg aaagcatcgt gctggtacgc ggtcgtgata aggttttgcg cgcgttctat
    12001 aacgtgtgtc cgcaccgtgg tcatcagttg ttgagcggtg aaggaaaagc aaaaaatgtg
    12061 attacctgcc catatcacgc atgggcattc aaactcgatg gcaatctggc ccatgcacgt
    12121 aactgcgaaa acgtcgccaa tttcgatagc gacaaagcgc aactggttcc ggtgcgtctg
    12181 gaggaatatg ccggattcgt cttcatcaac atggacccca acgccaccag cgtagaagat
    12241 cagttacccg gtctgggcgc gaaagtgctg gaagcctgcc cggaagtcca cgatctgaaa
    12301 ctggcggccc gctttaccac ccgcacgcct gccaactgga agaacattgt cgataactat
    12361 ctcgagtgct atcactgtgg tccggcgcat ccaggtttct ccgactccgt acaggttgat
    12421 cgctactggc acaccatgca cggtaactgg acgctgcaat acggtttcgc caaaccgtcc
    12481 gaacagtcgt ttaaatttga agagggtacg gatgcggcat tccacggttt ctggctgtgg
    12541 ccgtgcacga tgctgaacgt caccccgatc aaagggatga tgacggtcat ttatgaattc
    12601 ccggtggatt ctgaaactac cctgcaaaac tacgatattt acttcaccaa tgaagagtta
    12661 accgacgagc aaaaatcgct gattgagtgg tatcgcgatg tgttccgtcc ggaagattta
    12721 cgtctggttg aaagcgtaca gaaagggctg aaatcgcgtg gctatcgtgg tcaggggcgc
    12781 atcatggccg acagtagcgg tagtggcatt tccgaacatg gtatcgccca tttccataat
    12841 ctgctggcgc aggtgtttaa ggactaatga catcggcggc ggtattttcc gccgctgggc
    12901 tgatttttga tggagtacag caatgtcaga ctatcaaatg tttgaagtac aggtgagtca
    12961 ggtggaaccg cttaccgaac aggtgaaacg cttcacgctg gtggcaaccg atggcaaacc
    13021 gctgcctgcg ttcaccggag gaagtcacat cattgtgcag atgagcgatg gtgataacca
    13081 gtacagcaat gcgtattcac tactgagttc gccgcatgac acctcttgtt atcagattgc
    13141 cgttcggctg gaggaaaact cgcgcggcgg ttcccgcttt ttgcatcagc aggtaaaagt
    13201 gggcgatcgg ttaacgattt caacgcctaa taacctgttt gcgctaattc cctcagccag
    13261 aaagcatctg tttatcgcgg gcggtattgg tatcacccct ttcctgtcgc acatggcaga
    13321 gttgcaacac agcgacgtcg actggcagct acattactgc tcgcgaaatc cagaaagttg
    13381 cgcatttcgt gatgagctag tccagcatcc gcaggctgag aaagtccatt tgcatcattc
    13441 gtcaaccgga acacgactgg aattagcgcg attattggcg gatatcgaac ctggcacaca
    13501 cgtttatacc tgtggccccg aggcgctaaa tgaagcggta agaagtgaag ctgcgcgtct
    13561 ggacatcgcc gccgatacgc tgcactttga gcaatttgct atcgaagaca aaaccggcga
    13621 tgcctttacc ctggtgcttg cccgttccgg aaaagagttt gtggtgccgg aagagatgac
    13681 tattttgcag gttattgaaa ataataaagc cgcgaaagtg gaatgtttat gtcgtgaagg
    13741 ggtatgcgga acctgcgaaa cagcaatact ggaaggtgaa gctgaccatc gggatcagta
    13801 ttttagcgat gaagagcgtg ccagccagca aagtatgttg atctgttgtt cgcgtgcgaa
    13861 gggtaaacgc ctggtgttgg atttgtagtt tgccaaagcc ggatgtggcg ctgagcgcgc
    13921 ccagtccggc ttcggaagat tttactgcgg atattcctgc aataaattgt gtaatgcttc
    13981 cgccatcagc tcaccacgcc agccggaaat cagctccggc aaattgttct gcggtttcag
    14041 tttccagtgc cagttcagca gttggttgat ttgccgacgc gatgccagca attcggcgct
    14101 gatcttatgc gtttcgctca cgtcagtaat cagcgactta atcgctttaa acgctttacg
    14161 ataacccggc atgtccatca ggttaagcat cggctgcggt aaggcctctt ccggcaatgt
    14221 ctgcgctttt tccaccagcg ccagcagcgt tttaccgtga aagcggattt cgctaccgga
    14281 taaacccagg ctgtccagtt cgcctaaact tcccggcata taacgcgcga ccgaccacaa
    14341 atgctcttca cgcacgacaa agttcaccgc cagatcacgc tcacgagcct tgcgcagtcg
    14401 ccagtcggct aacagttgca gacaggccag ttggcgtgtg cgtaattgcc aggcattggt
    14461 gatatcacgc caggcatctt ccggcgcaac gacttcctga cgacgcattt gcatcaggcg
    14521 gcattcatcc agcgccgcag gtagccagcc ggaggcctcc gtttctacca taagcttggc
    14581 ggtgatcggt aacagatacc agacatccgc cgctgcgtat tcacactgac gttcggtcag
    14641 cggtctggcc agccagtcgg tgcgcgattc actcttgtcc agcgtaacgc cggaatactc
    14701 ttccaccatg gaagcgaaac cccatgacat cgggcgtccg cagaaggcag caaggatttg
    14761 cgtgtcaatc aggggttgtg gtaattcgcc aaagacattg aggaacactt ccagatcttc
    14821 actgcctgca tggagaaatt ttgtgatgga cggatcgcgc aggatcgctt tcagcggtga
    14881 ccagtcggtg atcccgagag gatcaattag cgccagatgc tcaccatcga aaagttgaat
    14941 caaccccagc tgcgggtaat aagtgcgcgt acgaacaaat tcagtatcct gggctatcgc
    15001 cggaaagaca cggacggctt cacacaaaga agccagcgca tcgtccgtgg taatcatttg
    15061 gtaattcaaa ttgtattctc tttagtgggc gtcaaaaaaa acgccggatt aaccggcgtc
    15121 tgacgactga cttaacgctc aggctttatt gtccactttg ccgcgcgctt cgtcacgtaa
    15181 ttctcgtcgc aaaatttttc cgacgttaga tttcggtaac tcatcacgaa actccaccag
    15241 cttcggtact ttgtagcccg tgagctgacg gcagcaaaag gtcaccagtg actcttcggt
    15301 aagcgatgga tcttttttca ctacgaagat tttcaccgct tcaccactgg agccggaagg
    15361 tacgccaaca gccgcgactt cctgtacgcc aggatgctgc atgacgacat cttcaatctc
    15421 gttgggatag acgttaaaac cggaaaccag aatcatgtct tttttacgat cgacaatgcg
    15481 caggaaccct tcttcatcca tcaccgcgat gtcgccggtg tgtaaccagc catttttgat
    15541 gatctcatcg gtagcatcgg gacgctgcca gtaacccagc atcacctgcg gtcctttgac
    15601 acaaagctca cccggttgac ccggtggtac ttcattatcg tcgtcatcca ccagtttggc
    15661 ttccgtcgac ggcaccggca aaccgatgct accactatga taatcaatat catatgggtt
    15721 aacgctgacc agcggcgcac actcggtaag accatagcct tccagcagat actgtcctgt
    15781 cagtttcacc caacgctctg ccaccacttg ctgcactggc atcccaccgc ctgcggaaag
    15841 atgcagactg gagaaatcca gctgctggaa ctctttattg ttcagcaacg cattgaacaa
    15901 ggtgttaacg ccagtgatag cggtaaacgg atatttcgct aactctttta ccaaccctgg
    15961 aatatcgcgc gggttagtga taagcaggtt ctgcccaccc agttcgataa acagcaggca
    16021 gttaatggtc agggcaaaaa tgtgatacag cggcagtgcc gttactacca gttctttacc
    16081 tggatgcagc aacggcccat aggtggcgtt gacctgttcc aggttcgcca gcatattgcg
    16141 gtgagtcagc atcgcgcctt tcgccacacc tgtggtgccg ccggtgtatt gcaggaaggc
    16201 taaatcttcc ggcaccagtt cgggtttgac gtactgcatc cggtagccgt tatgcagtgc
    16261 gctacgaaat gaaatggcat ctggcagatg gtatttcggc accaaacgct tgatgtattt
    16321 aacaacgaaa ttgactaccg tgccttttgc cgtagatagc tgatcgccca tacgggtcag
    16381 aattacgtgc tgaacggcgg ttttatcaac cactttttcc agtgtgtgag caaagttaga
    16441 cacgataaca atcgccgatg cgccgctatc gttaagctga tgctcaagct cacgcggggt
    16501 atacaacggg ttaacgttta cgacgatcat cccggcacgc aaaatgccaa acagcgccac
    16561 cggatattgc aataaattag gcatcatcaa cgcaacgcga tcgcctttct tcagccccaa
    16621 cccttgttgc aaataagcgg caaacgcgcg actgcgttct tccagcttgc ggaaggtcat
    16681 tacctccccc atattcacaa acgcaggctg atcggcgtag cggacgaccg actgctcaaa
    16741 catatctatc agagattgat aacggtcagg gttgatctcc gtcggaacat ccgcgggata
    16801 acggttaagc caaaccttct tcaattcttc acctctaaaa tgcgtgttcg tcgtcatcgc
    16861 aaccccaagt gataaacatg ttgttaacat aacattaact catcttacca gcttgataat
    16921 tagacaacga aaaggttgcg aagcgcgtca ctatttattt ttatagttgc cgtatgaacg
    16981 cagaaacagc ggaccagccg ctgtttcttt ttctttaaaa acaacttgtt actcggttac
    17041 aattgtttca actcttgcag ggccaggatt ataccaaccc catccgccgt aaccataggg
    17101 ccagccgcgt ccaccataaa accatggatc aattggctgc ggcggcataa tcacctgttg
    17161 agtgatatgc cagcgtttgt aacctgttac ctgcataacc ataaatttat atggtgcatt
    17221 atttactttg ccatccactg tgccagtgat tggcccgaca accgtcacca gttgtccacg
    17281 aaaatcgacc ggatcaagaa aaccgttcac atcggcataa atgcgcccgc gtgaaggttc
    17341 tcccaaggtt ggtcttgctc cgctgtccag cggtacggta gcaatttcca ggcgggtttt
    17401 cccttgctgg ttttgtacca caaccacttt gccgccaaag cgcgcctcct gaccaacata
    17461 taaatctggc gcattcatca cgcgtaccag atcttgctgt ggtgttggac tgctgccttt
    17521 aatggcgtcc ggcacagtga cacaaccgct cagcattagc gcaaacgtac ctgccagtat
    17581 gcctttgata acattctttt gaaccgccat gatgcgactc ctttttttct cagggcatac
    17641 tcttaagatt cattctttgc ccggaagttt cttccatgcg acgttgttac gtaaataaac
    17701 cggttcggca tgttccaccg ccaccgtttt accctcggca aacatctgac acgcaatcgg
    17761 cagcatatct tccgccgctg gcagtaaaac ttctccgtcg cgcaaaacca gcccgctctc
    17821 tttacggaga tccggccagg cttgccagcc cgtccctacc gttacccatt cgccggaaag
    17881 ctgttgcatt cgttcatgaa cgagttcggg tttgagtacg gcttcggttt cttcaccgcg
    17941 ccagataccg ttttcatcac gctgatattc ggcccagtaa acttcgccca ttcgcgcgtc
    18001 aatggctgcc agcacgcggg ttgcgccgtt tttgcgccac gccccctgcg ccatcgtcat
    18061 tagcgtagag acaccaatca tcggtaactc cgcgccaagc gccagccctt gcgcaatacc
    18121 aataccaatg cgcacgccag taaagctacc ggggccgcgc ccgtaagcca gagcgtttat
    18181 gtcagtcagg gaagttccgc tggtggtcag gatatcctgc accatcggta agattcgttg
    18241 agtatgttca cgagggcaaa gctcaaaatg agcgttgaca ttaccgtcgt tccacagagc
    18301 gacagagcag acctctgtcg cggtatcgat agccagaatt cgcatgggtc ttcgtgctta
    18361 gatcaataaa aaggcgcgca tcataccata ctccgtaaca aattacctgg aggatggtat
    18421 cgcaaggaaa cgaaccgcac gggcaatgtc acgggtgcgt ggcgcgggcg gcagactggc
    18481 gagaaacgtc gcgccgtaag gacgcatcac cagccgattg tcacaaatta ccagcacgcc
    18541 gcgatcgtcg gcgtcgcgaa tcagtcgccc tactccctgc ttgagagtaa tgaccgcatc
    18601 cggcagctgc acttcatcga acgggtcgcc accgcgcaaa cgacaatctt ccatgcgcgc
    18661 ttttaacagt agatcatccg gcgaggtaaa cggcaatttg tcgataatta ccaatgacaa
    18721 tgtatcgcca cgcacgtcca ccccttccca gaaactactg gttgccacaa gaagcgcgtt
    18781 accggcgctg acaaattgct gcaacagttg ccctttgctg gtttccccct gcaacaaaac
    18841 gggaagcgtc atggtagcgc ggaactgctc ggccagatcg cgcatcatgg catgcgaggt
    18901 acaaagcata aaacaacgac cgttgttagc ttcgatgatc ggtcgcagca ttgccgccag
    18961 ttgccgtgca caacccggtt gattggtttg cggcaggttg cgcggcacac agagtaacgc
    19021 ctggcggctg taatcaaacg ggcttggcaa tagcaacgac tccgcctgtt ctatcccaag
    19081 ccgcgaggtg aaatgatgca gatcgtcgtt caccgacagc gttgctgagg tgaagatcca
    19141 gctaccgggc ttttgcgcca ttaactcttt gaatttatcc gccacgctga gcggcgtgag
    19201 agccagagta aaatggcgcg aagtgcattc gtaccagtag ctgtagcccg gctgattgat
    19261 ctcttttagc cgcttcagcc gtgtgcgata caacgtggcg cgctcaaatg ccgcatccag
    19321 caaggcggaa cgccccagtg acagtttcgc cacgtcataa caaagttcca gggtgtcatc
    19381 gagcagtaaa aatgcccgct gaatttgcgg attagctaac agctcacgca ggttaccgcg
    19441 ataacctggc tcaccgagtt gcagacgaaa atcctgtgca ctctgggcaa gacgatcagc
    19501 gcacttttgt aactgctggg tgtcttttaa ttcggtgcgg taggcgatgg tgatgtcttt
    19561 tgccaggtcg agcagttgtc gactggagag tgactgacca aaatactggc tggcaatgtc
    19621 cggtagctgg tgggcttcgt cgaagatcat gacgtccgct tcagggatca actcgccaaa
    19681 tccactctct ttaaccacca tatccgccag aaagagatga tggtttacca ccaccacatc
    19741 ggcgtccatc gcttttttac gtgctttgac cacaaagcaa tctttataca tcgggcagtc
    19801 gctgccaaga cagttgtcgt tggtgctggt gaccagcggc cacgcctgtg aatcttccgc
    19861 cacgctgacg caggtgctga tatcaccatc gactgtttga ttagaccagg agcgcagcag
    19921 gatcacatcg cttaagattt gtaccggcag atcgcccccc gccagcgcct gctgttcgag
    19981 acgttcgagg cagaggtagt ttgagcgccc tttcagcagc gccacgttgc ccgtatattt
    20041 caatgccttt gagactgttg gcaaatcgcg gctgtagagc tgatcctgca acgcttttga
    20101 gccggtcgag ataatgactt tctttttcgc ccgcagcgca ggagccaggt aagcgtaggt
    20161 tttgcccgta ccggttcctg cttccaccac cagcggctgg cctttttcta tcgcctgggt
    20221 gacggctacc gccatctgtc gctgtggttc tcgcggctta aagcctggta tcgctttcgc
    20281 cagctgaccg tctggtgcaa aatcgtccgt cacactaccc cctgttgatt tgaacaggga
    20341 ttatgtcagg atgagggcgc tttcgccagt tgaagtggtg acggcaacct cacattgtgg
    20401 cagtctttgc agcacgaaaa tggaagtata atgaggacaa aatgatgact atcgttcgta
    20461 tcgatgctga agcccgctgg tctgatgtag taatccacaa caacacgctc tactacactg
    20521 gtgtaccgga aaacctcgac gccgatgcct ttgagcaaac cgccaacacg ctggcacaga
    20581 ttgacgccgt gctggaaaaa cagggcagca ataaatcgag cattctggat gccaccattt
    20641 tcctggccga taaaaacgac ttcgcggcga tgaataaagc gtgggatgcc tgggttgtcg
    20701 cgggtcatgc gccagtgcgc tgcacggtac aagcgggttt gatgaacccg aagtataaag
    20761 ttgaaattaa gattgtggct gcggtgtaag ctttatcgaa gcaaaataag tcagacgata
    20821 atttatcgat aatactggtc ggttttacat aaatcgacca gagaataaga tattactgtt
    20881 gatgcttctg ctaattcatt atttatattt ataatttcaa ttttatctat aaaactaact
    20941 atataaataa acataaccat gccgttgtgt tcgccctttg attagcaact ctggttttct
    21001 ttcttagctg aacaatctgg ttatagcgtg cggactgacc gttaattttc tccttcttac
    21061 tggataacac accatgagct gctaccttta aaacacctga atgtcaacag gttaactcgt
    21121 gccatatcgt tataatccct ttgatattag atgcaaatt
//
LOCUS       DADMKF010000064        21003 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.065, whole genome shotgun sequence.
ACCESSION   DADMKF010000064 DADMKF010000000
VERSION     DADMKF010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21003
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.065"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(<1..170)
                     /locus_tag="JNC24_002176"
     CDS             complement(<1..170)
                     /locus_tag="JNC24_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HAY9158917.1"
                     /translation="MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYG"
     gene            342..1562
                     /gene="coaBC"
                     /locus_tag="JNC24_002177"
     CDS             342..1562
                     /gene="coaBC"
                     /locus_tag="JNC24_002177"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HAY9158918.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEVAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            1540..1998
                     /gene="dut"
                     /locus_tag="JNC24_002178"
     CDS             1540..1998
                     /gene="dut"
                     /locus_tag="JNC24_002178"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HAY9158919.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDSTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            2105..2701
                     /gene="slmA"
                     /locus_tag="JNC24_002179"
     CDS             2105..2701
                     /gene="slmA"
                     /locus_tag="JNC24_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HAY9158920.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(2738..3379)
                     /gene="pyrE"
                     /locus_tag="JNC24_002180"
     CDS             complement(2738..3379)
                     /gene="pyrE"
                     /locus_tag="JNC24_002180"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HAY9158921.1"
                     /translation="MKPYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KNHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RSEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(3445..4161)
                     /gene="rph"
                     /locus_tag="JNC24_002181"
     CDS             complement(3445..4161)
                     /gene="rph"
                     /locus_tag="JNC24_002181"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HAY9158922.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAVCDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            4288..5151
                     /locus_tag="JNC24_002182"
     CDS             4288..5151
                     /locus_tag="JNC24_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HAY9158923.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            5373..6197
                     /gene="dinD"
                     /locus_tag="JNC24_002183"
     CDS             5373..6197
                     /gene="dinD"
                     /locus_tag="JNC24_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HAY9158924.1"
                     /translation="MNEHHQPFEEIRHYGTEGQEFWSARELAPLLDYRDWRNFQKVLA
                     RATQACEASNQAASDHFVETTKMVVLGSGAQRELEDVHLSRYACYLVVQNGDPAKPVI
                     AAGQTYFAIQTRRQELADDEAFRQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAI
                     DFAIFQNHGYRGLYGGLDQKAIHQRKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDGVNSKQQANTTHFDVGSKVRQTIQELGGTMPEELPTPQVSINQLENSVKITEKK"
     gene            6489..7106
                     /locus_tag="JNC24_002184"
     CDS             6489..7106
                     /locus_tag="JNC24_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HAY9158925.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(7103..8785)
                     /gene="ligB"
                     /locus_tag="JNC24_002185"
     CDS             complement(7103..8785)
                     /gene="ligB"
                     /locus_tag="JNC24_002185"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HAY9158926.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFGSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MVDKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVPQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQGNSDTLNSL
                     AVFVWAWPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEIARVRNEWWKAKLPFVT
                     DGVVVRAAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNSLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHQFNLARKQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQRLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            9043..9666
                     /gene="gmk"
                     /locus_tag="JNC24_002186"
     CDS             9043..9666
                     /gene="gmk"
                     /locus_tag="JNC24_002186"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HAY9158927.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            9721..9996
                     /gene="rpoZ"
                     /locus_tag="JNC24_002187"
     CDS             9721..9996
                     /gene="rpoZ"
                     /locus_tag="JNC24_002187"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAY9158928.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            10015..12123
                     /gene="spoT"
                     /locus_tag="JNC24_002188"
     CDS             10015..12123
                     /gene="spoT"
                     /locus_tag="JNC24_002188"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HAY9158929.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            12130..12819
                     /gene="trmH"
                     /locus_tag="JNC24_002189"
     CDS             12130..12819
                     /gene="trmH"
                     /locus_tag="JNC24_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HAY9158930.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            12825..14906
                     /gene="recG"
                     /locus_tag="JNC24_002190"
     CDS             12825..14906
                     /gene="recG"
                     /locus_tag="JNC24_002190"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HAY9158931.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDALKNKLLAALPFKPTGAQARV
                     VAEIERDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFEPLGIEVGWLAGKQKGKARLSQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACMTEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(15075..16280)
                     /gene="gltS"
                     /locus_tag="JNC24_002191"
     CDS             complement(15075..16280)
                     /gene="gltS"
                     /locus_tag="JNC24_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HAY9158932.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSIMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            16560..17951
                     /gene="xanP"
                     /locus_tag="JNC24_002192"
     CDS             16560..17951
                     /gene="xanP"
                     /locus_tag="JNC24_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HAY9158933.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            18072..19781
                     /locus_tag="JNC24_002193"
     CDS             18072..19781
                     /locus_tag="JNC24_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HAY9158934.1"
                     /translation="MKFIGKLLLYILIALLVAIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMVHRFSAPSHIVLENVTFGRDGQPATLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     PLTGDGNIQLTASGDIQANVPLKPTVSGQLHAVNAAKQQVTQTMNAGVVSSGEVTSAE
                     LVQ"
     gene            complement(19833..>21003)
                     /gene="yicI"
                     /locus_tag="JNC24_002194"
     CDS             complement(19833..>21003)
                     /gene="yicI"
                     /locus_tag="JNC24_002194"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HAY9158935.1"
                     /translation="LAIYDFTNPDACKWYADKLKGLVAMGVDCFKTDFGERIPTDVQW
                     FDGSDPQKMHNHYAYIYNELVWNVLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYA
                     NYESMAESLRGGLSIGLSGFGFWSHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSK
                     SYRVPWAYDDESCDVVRFFTQLKCRMMPYLYREAARAKARGTPMMRAMMMEFPDDPAC
                     DYLDRQYMLGDNVMVAPVFTEAGEVQFYLPEGRWTHLWHNNELDGSRWHKQQHGFLSL
                     PVYVRDNTLLALGNNDQRPDYAWHEGTAFHLFNLQDGHEAVCEVPAADGSVIFTLKAT
                     RTGNTITVTGAGEARNWTLCLRNIVKVNGLQGGSQAESEQGLVVTPQGNALTITL"
BASE COUNT         5082 a         5713 c         5386 g         4822 t
ORIGIN      
        1 ccataaagag agccgaaatt ctccagcatc tcttttgcca gggttaatac atctttaccg
       61 cgcgttccgg tacgcagaaa tagcgccagc agctcgacat ccgttaaggc gctaatacca
      121 aacttcagca ttttttcgcg cggcatcaac agctgtgcat tgtttttcac cttcacctcc
      181 tttgtggtgc tcgcatcctg tcatagccgt ttcgttaaat cgacggccag ttttcaatcc
      241 tggaaagcgc ctcgcaaagt gaatctcagg tgaaggcacg acaacaaaag tattgtgata
      301 aaatcgccaa cttctggtgt caccctacag gaaaaatcat catgagcctg gccggtaaaa
      361 aaatcgttct cggcgttagc ggcggtattg ctgcctataa aacccctgaa ctggtgcgtc
      421 gtttgcgcga tcgcggggcc gacgtccgcg tagccatgac cgaagcggca aaagccttta
      481 tcaccccact tagcttgcag gcggtttctg gttatcccgt ttccgacagt ctgctggacc
      541 cggcagccga agccgctatg ggccatattg agctgggtaa atgggctgat ttagtgattc
      601 tcgcccctgc cacggcagat ttgattgccc gtgttgctgc cggaatggcg aatgacctgg
      661 tatcgacgat ttgtctggct acacctgcgc ctgtagccgt gctccccgcc atgaaccagc
      721 agatgtaccg tgccgctgcc acgcagcata atttagaggt gcttgcttcc cgtggtttgc
      781 tcatctgggg gccagacagt ggcagtcagg cttgtggtga tatcggtcct gggcgaatgc
      841 tcgatccgtt aaccattgtg gatatggcgg tagcgcattt ttcgcccgtc aacgacctga
      901 aacatctgaa cattatgatt accgccggcc cgacgcgtga accgctcgat ccggtgcgtt
      961 atatctctaa tcacagctcc ggcaagatgg gttttgctat cgccgccgcc gctgcccgtc
     1021 gtggcgcgaa cgtcacgctg gtatcaggcc cggtttcgct accgacgcca ccgtttgtta
     1081 aacgtgttga tgtgatgacc gcgctggaaa tggaagtcgc cgtgaatgct tctgtacagc
     1141 agcaaaatat ttttatcggc tgcgccgccg tggcggatta tcgcgcagct accgtggccc
     1201 cagagaaaat caaaaagcag gccacgcagg gtgatgaatt aacaataaaa atggttaaaa
     1261 accccgatat cgtcgcaggc gttgccgcac taaaagacca tcgaccctac gtcgttggat
     1321 ttgccgccga aacaaataat gtggaagaat acgcccggca aaaacgtatc cgtaaaaacc
     1381 ttgatctgat ctgcgcgaac gatgtttccc agccaactca aggatttaac agcgacaaca
     1441 acgcattaca ccttttctgg caggacggag ataaagtctt accgcttgag cgcaaagagc
     1501 tccttggcca attattactc gacgagatcg tgacccgtta tgatgaaaaa aatcgacgtt
     1561 aagattctgg acccgcgcgt tgggaaggaa tttccgctcc cgacttatgc cacctctggc
     1621 tctgccggac ttgacctgcg tgcctgtctc gacgacgccg tagaactggc tccgggtgac
     1681 tctacgctgg ttccgaccgg gctggcgatt catattgccg atccttcact ggcggcaatg
     1741 atgctgccgc gctccggatt gggacataag cacggtatcg tgcttggtaa cctggtagga
     1801 ttgatcgatt ctgactatca gggccagttg atgatttccg tgtggaaccg tggtcaggac
     1861 agcttcacca ttcaacctgg cgaacgcatc gcccagatga tttttgttcc ggtagtacag
     1921 gctgaattta atctggtgga agatttcgac gccaccgacc gcggtgaagg cggctttggt
     1981 cactctggtc gtcagtaaca catacgcatc cgaataacgt cataacatag ccgcaaacat
     2041 ttcgtttgcg gtcatagcgt gggtgccgcc tggcaagtgc ttattttcag gggtattttg
     2101 taacatggca gaaaaacaaa ctgcgaaaag gaaccgtcgc gaggaaatac ttcagtctct
     2161 ggcgctgatg ctggaatcca gcgatggaag ccaacgtatc acgacggcaa aactggccgc
     2221 ctctgtcggc gtttccgaag cggcactgta tcgccacttc cccagtaaga cccgcatgtt
     2281 cgatagtctg attgagttta tcgaagatag cctgattact cgcatcaacc tgattctgaa
     2341 agatgagaaa gacaccacag cgcgcctgcg tctgattgtg ttgctgcttc tcggttttgg
     2401 tgagcgtaat cctggcctga cccgcatcct cactggtcat gcgctaatgt ttgaacagga
     2461 tcgcctgcaa gggcgcatca accagctgtt cgagcgtatt gaagcgcagc tgcgccaggt
     2521 attgcgtgaa aagagaatgc gtgagggtga aggttacacc accgatgaaa ccctgctggc
     2581 aagccagatc ctggccttct gtgaaggtat gctgtcacgt tttgtccgca gcgaatttaa
     2641 ataccgcccg acggatgatt ttgacgcccg ctggccgcta attgccgcgc agttgcagta
     2701 atatgacgcc ggatgacttt tcatccggcg agtttcttta aacgccaaac tcttcgcgat
     2761 aggccttaac cgccgccaga tgttccgcca tttccggctt ctcttccagg taagcaatca
     2821 ggtctttcag ggtgatgata gagatcactt tgcagttgta atcacgctca acttcctgaa
     2881 tcgccgaaat ctcgctgcgc ccccgttcct gacgatcgag cgaaatcaac acgccagcaa
     2941 gcgtcgcgcc attggcctga ataatttcca tcgactcacg aatcgccgtt ccggcggtga
     3001 tcacatcatc taccagcatt acgcgtcctt gtaacgcgct accaaccaga ttgccgcctt
     3061 caccgtggtt ttttgcctct ttgcggttaa agcagtacgg caggtccagg tcatgatgct
     3121 ccgccagcgc cacggcggtt gtggtggcaa tcgggatccc tttgtaagca gggccaaaca
     3181 gcagatcgaa ctcaatgccg gaatccacca atgcttcagc gtaaaaacgg cctaacagtg
     3241 ccaggtcgcg cccggtatta aacagcccgg cgttgaagaa ataggggctt ttacgcccgg
     3301 atttcagcgt aaactcgcca aactttaaca cctgcttgct aagcgcaaat tcaataaact
     3361 ggcgctgata tggtttcatg ccttcgctcc tcatcttact tttctacaga caaaaaaaag
     3421 gcgactcatc agtcgcctta aaaatcagtt tgccagcgcc gccttctgcg tcgctacaat
     3481 ggattcgatt ccccctcggg ccagagccaa caaggtgagt aattcttcgt gggtgaacgg
     3541 ttcgccttct gccgtcccct gcacttcaat gatgcgcccg tcttcggtca tcactacgtt
     3601 catgtcggtc tctgcggcag agtcttcaac gtattccaga tcgcaaaccg cttcgccgtt
     3661 cacaatcccg acagaaaccg cggctaccat ccctttcatc ggattggttt tcagcttacc
     3721 gttttccacc agcttctgta gcgcatctgc cagcgccacg caggcacccg taatcgaagc
     3781 ggtacgagtg ccaccatcag cctgaagcac gtcgcagtcc agcgtaatgg tgaactcacc
     3841 cagcgctttc aaatctactg ccgcgcgaag cgcacgggcg atcagacgct ggatttccat
     3901 tgtgcgtcca ccctgcttac ctttcgccgc ttcacgagcg ttacgggtgt gggtagaacg
     3961 cggcagcata ccgtactctg cggtgatcca gccctggccc tgacctttca ggaagcgcgg
     4021 cacgccttct tcaatagagg cggtacacaa cactttggta tcgccaaatt cgaccagcac
     4081 cgagccttct gcatgttttg tatagttacg agtcagggta acgggacgca cctgattatt
     4141 gctacggcct gctggacgca tattgaaatc tccggcttga aacaaatgtg gctgcgcatt
     4201 atacggactt ccggaggtta ttcctatcct gacaaggcat cgatggctat aatccttcca
     4261 cctctccttt tataaacagg aacgtctatg atccgcagta tgaccgccta cgcccggcgt
     4321 gaaatcaagg gtgaatgggg gagcgcaacc tgggaaatgc gctcggtaaa ccagcgttat
     4381 ctggaaactt actttcgtct gccggagcag ttccgtagcc ttgaacctgt cgttcgcgag
     4441 cgtattcgtt ctcgcctgac gcgcggtaaa gtggaatgta ccctgcgcta tgagccagat
     4501 gttagcgcgc aaggtgagct gatcctcaac gaaaaactgg ctaaacagct ggtaactgcc
     4561 gcgaactggg taaaaatgca gagtgacgaa ggggaaatca acccggttga tattctgcgc
     4621 tggccgggcg taatggcagc ccaggagcag gatctcgacg cgattgccgc tgaaattctc
     4681 gcggcgttgg atggtaccct ggacgacttt atcgtcgcac gtgaaaccga aggtcaggcg
     4741 ctgaaagcgt tgatcgagca gcgtctggaa ggcgtcaccg ccgaagtggt caaagtccgc
     4801 gcccatatgc cggaaatcct gcaatggcag cgtgagcgtc tggtcgcgaa gctggaagat
     4861 gctcaagtgc aactggaaaa caaccgcctg gagcaggagt tggttctgct ggcacaacga
     4921 attgatgttg ccgaagaact ggaccgcctg gaagcgcatg tcaaagagac ctacaacatt
     4981 ctgaagaaaa aagaagccgt tggtcgtcgt ctggacttta tgatgcaaga gttcaaccgc
     5041 gagtcgaaca ctcttgcgtc gaaatctatc aatgccgaag tgacgaattc tgccatcgag
     5101 ctgaaagtgt tgattgagca gatgcgcgag cagattcaga atattgagta actcgtaaca
     5161 ctaattctta cctttcagcc ttcacaaatg ccgcccactc aaacagagcg gcatttttct
     5221 tccccgcaac attcaattct gttttgcgta cctgctccag attttgcgat gtttttttgc
     5281 gcagcgcagt gagaacgtga gatactcaca actgtatata aatacagtta tagacttact
     5341 ttctttgcaa tcgatatcac atggagtggg caatgaacga acatcatcaa ccttttgaag
     5401 aaatacggca ttatggcact gaagggcaag aattttggtc agcgagagaa ttggcaccgt
     5461 tactggatta tcgggactgg cgcaattttc aaaaggtact ggcccgcgcg actcaagcgt
     5521 gcgaagccag caatcaagct gcatctgacc atttcgttga aaccaccaaa atggttgtat
     5581 taggttcagg ggctcaacga gaacttgaag atgttcatct ctctcgctat gcctgttacc
     5641 tggtagtaca aaacggcgac cctgcgaaac cggttattgc ggcagggcaa acttattttg
     5701 ctatccagac ccgacggcag gagctggctg acgatgaagc tttcaggcaa cttcgtgaag
     5761 acgaaaagcg tctttttctg agaaatgagt tgaaagaaca taacaaacaa ttagttgagg
     5821 cagcgcagca agctggggta gcgacagcta tcgatttcgc catatttcag aatcatggtt
     5881 accgggggct gtatggtgga ttagatcaga aagccatcca tcagcggaag gggctgaaaa
     5941 agagtcagaa gatcctggat catatgggtt caacagaact ggcggctaat ctctttcgag
     6001 ctacccaaac agaagaaaaa ctcaagcggg atggcgtaaa ttcaaaacag caagctaata
     6061 ccacacactt tgacgtgggt agcaaggtga ggcaaaccat tcaggaactt ggcggaacca
     6121 tgcctgagga gttgccgacc ccgcaggtca gcatcaatca gttggaaaac agtgtaaaaa
     6181 ttacagagaa gaaatagcag aaactaaaaa ctctttttct taattgagat acccgatgcg
     6241 taagccaaag tccaggtgca gtgaacacaa tggctaaaca ttgcaccttt cttttccacc
     6301 cctcaatttt aacctatttt ttcttatgca ttttctcaga caagaagtca gaagaatgca
     6361 tctacactac agaaaatagc gatttcacat aactacaagt tatcaatttc ctctcacctt
     6421 aaaaaatctc aatcgtgaca atacgcacaa atcgctaccc tgccagacag atttttaggg
     6481 agagaaccat gctgttacac attttgtatt tggttggcat tactgccgaa gccatgacgg
     6541 gggcgctggc ggccggacga cggcgcatgg atacatttgg cgtaattatt attgcgaccg
     6601 ccaccgcaat tggcggaggg tcagtgcgcg atattctgct gggccactat ccgctcggtt
     6661 gggtcaaaca cccggaatat gtgattatcg tcgctaccgc cgcagtactc accactatcg
     6721 ttgctcctgt aatgccttac ctgcgcaaag tgtttctggt gctcgatgcg ctcggactgg
     6781 tggtcttttc tatcatcggc gcacaggttg cactggatat ggggcacggc ccaattattg
     6841 ccgttgtcgc ggcggtaacc accggcgtgt ttggcggcgt tttgcgcgat atgttctgta
     6901 aacgcatccc actggtattc cagaaagagt tgtatgccgg tgtctcattt gcctccgccg
     6961 tgctgtacat cgcgctgcaa cactatgttt ctaaccatga tgtggtgatt atctccaccc
     7021 tggtattcgg cttttttgcc cgcttactgg cgctacgtct taagctggga ttaccggtct
     7081 tttactacag ccacgaaggt cactaaggtt caaaacctgt gatctgctgg gcagccagcc
     7141 aactgcccag cttcttgatt tgcgcatttt ccttccattc aataacctgt ctggcgcgcc
     7201 cagatccagt ccccggcagc cgctgccaga actgctccgt gctaaataaa agttgcgacc
     7261 aggaccgttc atcactggca ttaagcgccg cccgggttaa cggtattccc attgccatca
     7321 cccagcgagt aaaaggctgc ttacgagcca gattaaactg atgccataac tgcgcacttt
     7381 tacttttcgc gatccccggc gtgttctgta attgctctgg cgttaataaa agccaggaaa
     7441 agatatgctc aaagcgatga gtctgatgca gcgcgcgcca gccggcctca ccaatgccat
     7501 ccagcccaag aacctgtttt gaccccagcc agactaagcg tgaaatgaac tgttcctgac
     7561 aaacatcaga agcaaagtag caggtcaacg agttaaagcg gttttctggt ggtgtcggtt
     7621 ttgtacgttc tgcaccgcgc cagaccacat catcaatgcg aggaataccc tgaccggcaa
     7681 ggctgacgag gatctgatca ccaggcgcaa tatcccactc ctgccagcgc ctgacggaac
     7741 caatattcac ccgctggact tttttatcat ccagcatgac aggtgcgagt gacgcaacca
     7801 ccgatatttt accgctctta cccaccgcaa actgaatcgc cttcacttcg gcaacctgag
     7861 ctacaggttg atatttccag gccaccaacc attctgcctg gcctggtagc caatggcggg
     7921 attcgggttc cttagccgct cgcacaacca cgccatcggt gacgaagggt aatttcgctt
     7981 tccaccactc attgcgtacg cgcgcaattt catcagcatt tttaaccgca cgggtatacc
     8041 tctgcgttag agtaaaacct gcggtagcca gctcctttaa acgatcggtc attaactgcg
     8101 gtccatccgg ccatgcccag acaaaaacag ccagtgagtt tagcgtgtcg ctattcccct
     8161 ggcgcatcat caggccagca acttttgcgc gggcatttat tccccccatt tgttgttgga
     8221 tatgcccctc gcgctggaga aatatttccc cctgaagcgt actgttggct aaaggcccgc
     8281 taacggtttg cggcacagcg gaaattaagc taactttctg cgtccagtcc tcgcctttca
     8341 ggccattacc gcgactgatt gctttgttca gtttcccgtc ccgataaacc agggttaccg
     8401 caacgccatc aacttttggc tgcacccaaa gatcgctacg ttctcgcatc cacagactta
     8461 atgcgttttt atccaccatt ttgcgtacac ccgtatgggc aaccggatgc ataaccgcac
     8521 catttaaagg cggcatcatc acgtcgcggg gctcgctccc aaagcagcgt tgccactgcg
     8581 taagacgggc gcttaactga tcgtaaacac cgtcttccac ctcacttttc ccttccttcc
     8641 agtagtcatc gtcccactgt tttatttgct gttgcaggcg ggaaatttct tcctgtgctc
     8701 tggctggcga ccaggccgga cagaccgccc acacagatga ttgccagcac aagatactta
     8761 ttaatatcgc catccatact ttcatcatca cctccgctgt agatagtcag gcagatatac
     8821 aacgtgatga aagcaaagcc gagtggcaaa aacggagtct gcgaggacgc ttcctgaaaa
     8881 tcgtctttat tgcaatgaat gacaggcaaa tgcggaagca gctacgcaaa acgcaacaac
     8941 tttgcgcaaa aagtgtgagc aagggctacg tcacatggcc gcgccgtgta taataagctc
     9001 gtatgtaggc tttatttcgc taatcacata cgaaagatac tcatggctca aggcacgctt
     9061 tatattgttt ctgcccccag tggtgcgggt aaatccagcc tgattcaggc tttattaaaa
     9121 acccaaccgt tgtatgacac ccaggtttct gtttcacaca ccacacgcca accgcgtcct
     9181 ggtgaagtcc acggtgaaca ttatttcttt gttaatcatg atgaatttaa agaaatgatt
     9241 agcagagatg cgttcctcga acacgcagaa gtttttggta attactatgg cacttcgcgt
     9301 gaggccattg agcaagtact ggcgaccggt gtcgatgttt ttctcgatat cgactggcag
     9361 ggcgcgcagc aaattcgcca gaagatgccg cacgcgcgga gtatctttat tttaccgccg
     9421 tccaaaattg aactggaccg ccgtctacgc ggtcgcggtc aggacagcga agaggtcatt
     9481 gcaaagcgta tggcgcaagc tgttgcagaa atgagccatt acgccgaata tgattatctg
     9541 attgtgaatg atgacttcga taccgcgttg accgatttga agaccattat tcgcgccgaa
     9601 cgtctgcgca tgagccgcca aaagcagcgt catgacgctt taatcagcaa attgttggca
     9661 gactgaacct gatttcagta tcatgcccag tcatttcttc acctgtggag ctttttaagt
     9721 atggcacgcg taactgttca ggacgctgta gagaaaattg gtaaccgttt tgacctggta
     9781 ctggtcgccg cgcgtcgcgc tcgtcagatg caggtaggcg gaaaggatcc gctggtaccg
     9841 gaagaaaacg ataaaaccac tgtaatcgcg ctgcgcgaaa tcgaagaagg tctgatcaac
     9901 aaccagatcc tcgacgttcg cgaacgccag gaacagcaag agcaggaagc cgctgaatta
     9961 caagccgtta ccgctattgc tgaaggtcgt cgttaatcac aaagcgggtc gcccttgtat
    10021 ctgtttgaaa gcctgaatca actgattcaa acctacctgc cggaagacca aatcaagcgt
    10081 ctgcggcagg cgtatctcgt tgcacgtgat gctcacgagg ggcaaacacg ttcaagcggt
    10141 gaaccctaca tcacgcaccc ggtagcggtt gcctgcattc tggccgagat gaaactcgac
    10201 tatgaaacgc tgatggcggc gctgctgcat gacgtgattg aagatacacc cgccacctac
    10261 caggatatgg aacagctttt tggtaaaagc gtcgccgagc tggtagaggg ggtatcgaaa
    10321 cttgataaac tcaagttccg cgataagaaa gaggcgcagg ccgaaaactt tcgcaagatg
    10381 attatggcga tggtgcagga tatccgcgtc atcctcatca aacttgccga ccgtacccac
    10441 aacatgcgca cgctgggctc acttcgcccg gacaaacgtc gccgcatcgc ccgtgaaact
    10501 ctcgaaattt acagcccgct ggcgcaccgt ttaggtatcc accacattaa aaccgaactc
    10561 gaagagctgg gttttgaggc gctgtatccc aaccgttatc gcgtaatcaa agaagtggtg
    10621 aaagccgcgc gcggcaaccg taaagagatg atccagaaga ttctttctga aatcgaaggg
    10681 cgtttgcagg aagcgggaat accgtgccgc gtcagtggtc gcgaaaagca tctttattcg
    10741 atttactgca aaatggtgct caaagagcag cgttttcact caatcatgga catctacgct
    10801 ttccgcgtga tcgtcaatga ttccgacacc tgttatcgcg tgctcgggca aatgcatagc
    10861 ctgtacaagc cgcgtccggg tcgcgtgaaa gactacatcg ccattccaaa agcgaacggc
    10921 tatcagtctt tgcacacctc gatgatcggc ccgcacggtg tgccggttga ggtccagatc
    10981 cgtactgaag atatggatca gatggcggag atgggtgttg ccgcgcactg ggcttataaa
    11041 gagcacggcg aaaccagcac caccgcgcaa atccgcgccc agcgctggat gcaaagcttg
    11101 ctggagctgc aacagagcgc cggtagttcg tttgaattta tcgagagcgt taaatccgat
    11161 ctcttcccgg atgagattta cgttttcaca ccggaagggc gaattgtcga gctgcctgcc
    11221 ggtgcaacgc ccgtcgactt cgcttatgca gtgcataccg atatcggtca tgcctgcgtg
    11281 ggcgcacgcg ttgaccgcca gccttacccg ctgtcgcagc cgcttaccag cggtcaaacc
    11341 gttgaaatca ttaccgcacc gggcgctcgc ccgaatgccg cgtggctgaa ctttgtcgtt
    11401 agctcaaaag cgcgcgccaa aattcgtcag ttgctgaaaa acctcaagcg tgatgattct
    11461 gtaagcctgg gccgtcgtct gctcaaccat gctttgggtg gtagccgtaa gctgaatgaa
    11521 atcccgcagg aaaatattca gcgcgagctg gatcgcatga agctggcaac gcttgacgat
    11581 ctgctggcag aaatcggact tggtaacgca atgagcgtgg tggtcgcgaa aaatctgcaa
    11641 catggggacg cctccattcc accggcaacc caaagccacg gacatctgcc cattaaaggt
    11701 gccgatggcg tgctgatcac ctttgcgaaa tgctgccgcc ctattcctgg cgacccgatt
    11761 atcgcccacg tcagccccgg taaaggtctg gtgatccacc atgaatcctg ccgtaatatc
    11821 cgtggctacc agaaagagcc agagaagttt atggctgtgg aatgggataa agagacggcg
    11881 caggagttca tcaccgaaat caaggtggag atgttcaatc atcagggcgc gctggcaaac
    11941 ctgacggcgg caattaacac cacgacttcg aatattcaaa gtttgaatac ggaagagaaa
    12001 gatggtcgcg tctacagcgc ctttattcgt ctgaccgccc gtgaccgtgt gcatctggcg
    12061 aatatcatgc gcaaaatccg cgtgatgcca gacgtgatta aagtcacccg aaaccgaaat
    12121 taatgtttta tgaacccaac acgttatgca cgcatctgcg aaatgctcgc caggcggcag
    12181 cctgatctga ccgtctgcat ggagcaggtc cacaaacctc ataacgtttc tgcgattatt
    12241 cgtaccgcag atgccgttgg cgtacatgaa gttcacgccg tctggcctgg tagccgcatg
    12301 cgcaccatgg cttcggcagc ggcgggtagt aacagctggg tacaggtgaa aacacaccgc
    12361 accattggcg atgccgtcgc tcatctgaaa ggccagggta tgcagattct ggcaacccat
    12421 ctttctgata acgctgtcga tttccgcgaa attgattaca ctcgcccgac ctgcattttg
    12481 atgggacagg agaaaacggg catcacgcag gaagcattgg ccctggcgga tcaggacatc
    12541 atcattccga tgatcggcat ggtgcagtcg ctgaatgttt ccgttgcctc agccctcatt
    12601 ctttacgaag cccagcgtca gcggcaaaat gcaggcatgt acctgcgtga aaacagcatg
    12661 ttgccggaag cagagcaaca acgcctgttg tttgaaggcg gctatccggt gctggcgaaa
    12721 gtcgcaaaac gcaaaggcct gccttatccc cacgtcaatc agcaaggcga gatcgaagct
    12781 gatgccgact ggtgggctac tatgcaggct gcagggtaag tgccatgaaa ggtcgcctgt
    12841 tagatgctgt cccactcagt tccctaacgg gcgttggcgc agcacttagt aacaaactgg
    12901 cgaaaatcaa cctgcatacc gtgcaggatc tactcttaca ccttcccctg cgctacgaag
    12961 atcgcaccca tctctacccc atcggagaac tactgccggg cgtttatgcc acggtggaag
    13021 gcgaagtgct gaactgcaat atctccttcg gcggtcggcg gatgatgacc tgccagatca
    13081 gcgacggttc cggcatcctc accatgcgct ttttcaattt cagcgcggca atgaaaaata
    13141 gcctggcgac gggccgccgt gtactggctt atggcgaagc aaagcgcggt aaatatggtg
    13201 cggagatgat ccacccggaa taccgcgtgc agggcgatct cagcacgcca gaattacagg
    13261 aaacgctcac gccggtttat ccaacaacgg aaggcgtaaa gcaggccacg ctgcgtaaat
    13321 taaccgacca ggcgctggat ctgctcgaca cctgcgccat tgaagaactc ctgccgccgg
    13381 aactgtcaca aggaatgatg acgctaccgg aagcgttgcg cactttgcac cgcccgccac
    13441 cgacgctaca gcttagcgat ctggaaaccg ggcagcatcc ggcgcaacgt cgtctgattc
    13501 tggaagaact gctggcgcac aacctcagca tgttagcctt acgtgccgga gcacagcgtt
    13561 ttcatgccca gccgctgagc gccaatgatg cgctgaaaaa taaactcctc gcagccttac
    13621 cgttcaagcc aacgggcgca caggcacgcg tagtggcgga gatcgagcgc gatatggcgc
    13681 tggatgtgcc gatgatgcgc ctggtacagg gcgatgtagg ttccggtaaa acgctggtcg
    13741 ccgccctcgc cgcgctgcgt gcgattgccc acggcaaaca ggtggcattg atggcaccaa
    13801 ccgaattact cgccgaacag cacgccaata acttccgcaa ctggtttgaa ccgctcggta
    13861 tcgaagtggg ctggctcgcc ggtaagcaga aaggcaaagc gcgactgtct cagcaggaag
    13921 ccatcgccag cggtcaggtg cagatgattg ttggtacgca cgccatcttc caggaacagg
    13981 tgcagtttaa cggtctggcg ctggtgatta tcgacgaaca acatcgcttt ggcgtgcatc
    14041 agcgtctggc gttatgggag aaaggccagc agcaaggctt ccatccgcat cagttgatca
    14101 tgaccgccac gccgatcccc cgcacgctgg caatgacggc gtatgccgat ctcgatacct
    14161 cggtgataga tgaactgccg ccaggccgca cgccagtgac tacggtcgct attcctgata
    14221 ctcgccgtac cgacatcatt gaccgcgtgc gccacgcctg catgactgaa ggtcgtcagg
    14281 cgtactgggt ttgtacgttg attgaagagt cagagttact ggaagcgcag gcggcggaag
    14341 ctacctggga agagttgaaa ctggcgctac cagaattgaa tgttggcctg gtacacgggc
    14401 ggatgaaacc agccgagaaa caggcggtga tggcgtcgtt taaacaaggt gagctacatc
    14461 tgctggttgc cactaccgtt attgaagtcg gtgttgatgt gcctaacgcc agcctgatga
    14521 ttatcgaaaa ccctgagcgt ctgggtctgg cgcagttaca ccagctgcgc gggcgcgtag
    14581 gtcgtggcgc ggtggcttct cactgcgtgc tactctacaa aacgccgctt tctaaaacgg
    14641 cgcaaattcg cctgcaagtg ctgcgcgaca gtaacgacgg ttttgtgatt gcgcaaaaag
    14701 atctggagat tcgcggccct ggcgaattgt taggcacgcg tcagacgggt aatgctgaat
    14761 ttaaagtggc ggatttactg cgcgatcagg cgatgatccc ggaagttcag cgcctggcac
    14821 gccatattca cgaacgttac ccacaacagg caaaagccct gatagaacgc tggatgccgg
    14881 agacggaacg ttactcgaat gcgtaaaagg cggcagtgag aagaccgcca tttcaggtta
    14941 ccctaccttc ctgccggatg cgattcatca ccctacaaat tcaataaatt atgaatcaat
    15001 acgcaggctt gataagcgaa gcgtatcagg catttgtctt tgttgcggcg gcacttctac
    15061 cgccgcttca tcggttaacc ggcaaaaatc ggcaacatca aatacaactt aattaccagc
    15121 gcattgacga tatcgataaa gaacgcaccg accatcggca ccaccaaaaa cgccatgtgc
    15181 gacgggccaa agcgttcagt aatcgcctgc atgttggcga ttgccgttgg cgttgcaccg
    15241 aggccaaaac cacagtgacc tgcagccagc actgccgcat cgtagttttt gcccatcatg
    15301 cgccaggtaa cgaagatggc atacaacgcc atgaagatgg tctgtaccac cagaatcgcc
    15361 agcatcggca gcgccagcga agccagctcc cacagtttca gccccatcaa cgccatcgcc
    15421 aggaacaacg acaagcttac gttacccagc acggataccg cacgctcaaa gacgcggtaa
    15481 aagcccatta ttgacagacc gttgctcaga atcacgccaa caaacagtac acagacgaag
    15541 gtcggcagtt caaaagcagt gccagccaaa agttgcgcaa caattttccc caccgtcagg
    15601 cagatagcaa tcagcgcgat agtttcaatc agcaccaacg aggtgatcat gcgtcccaca
    15661 tccggctttt caaacgccgt cgggacttcc tggtcatccg gaataccgtt cggcgtggtg
    15721 gagtgtttca ccagatagcg cgccaccgga ccgccaatca agccgcccag caccagaccg
    15781 aacgttgcac aggccatcgc cacttccgtc gcattggtga agccataacg ttcaatgaac
    15841 aatttactcc acgcagcgcc cgtaccgtga ccgccggaaa gcgtaataga accggccaac
    15901 agccccatca gcggatcaag ccctaacagg ctagccatac caatgccaat ggcattttgc
    15961 atcaccaaca gaccaacaac cacaatcaag aagatgccaa ccacacgccc accggcacgc
    16021 aaactggcaa tgttggcgtt caggccaatg gtggcgaaga aagccagcat taacggatcg
    16081 cgcagggaca tatcaaagtt gacttcccag cccatgcttt ttttcagtac tagtagcgcc
    16141 agcgccacca acaaaccacc cgcaacaggt tccggtatgg tgtatttctt caaaaaggag
    16201 acggaatgga ccaacttacg cccgagcagc aacgtcagcg ttgcggcaac aagcgttgct
    16261 aaagtatcga gatgaaacat agttactcct ttgtatccgc atcactcttt catactcgtc
    16321 atacttcatc gcgcagtggc aacttcatga actatgacgg gtatcgagtt atgctttcct
    16381 gggtcaaatt ctcagctgtt aatgaacaaa tggcatggat tttaagcaga aaaataccaa
    16441 aagttatata aaaaagtgca tttatctcat ttaaatattt agatgaggat gtggcaaacg
    16501 tttgcttttc tctttcggtc agctaaaatg cccgctttgc taccacggga ttgttttcga
    16561 tgtctgtttc caccctcgag tcagaaaatg cgcaaccggt tgcgcagact caaaacagcg
    16621 aactgattta ccgtcttgaa gatcgtccgc cgcttcctca aaccctgttt gccgcctgtc
    16681 agcatctgct ggcgatgttc gttgcggtga tcacgccagc gctattaatc tgccaggcgc
    16741 tgggtttacc ggcacaagac acgcaacaca ttattagtat gtcgctgttt gcctccggtg
    16801 tggcatcgat tattcaaatt aaggcctggg gtccggttgg ctccgggctg ttgtctattc
    16861 agggcaccag cttcaacttt gttgccccgc tgattatggg cggtaccgcg ctgaaaaccg
    16921 gtggtgctga tgttcctacc atgatggcgg ctttgttcgg cacgttgatg ctggcaagtt
    16981 gcaccgagat ggtgatctcc cgcgttctgc atctggcgcg ccgcattatt acgccgctgg
    17041 tttctggcgt tgtggtgatg attatcggcc tgtcgctaat tcaggtgggg ctgacctcca
    17101 ttggcggcgg ttacgcagcc atgagcgata acaccttcgg cgcaccgaaa aatctgctgc
    17161 tggcaggcgt ggttttagcc ttaattatcc tgcttaaccg tcaacgtaac ccttacttac
    17221 gcgtggcctc actggtaatt gcgatggcgg ccggatatgc gctggcgtgg tttatgggca
    17281 tgttgccaga aagcaacgaa ccgatgacgc aagaactgat tatggtgcca acgccgctct
    17341 attacggtct tggcattgaa tggagtctgc tgctgccgct gatgctggtc tttatgatca
    17401 cttcgctgga aaccattggc gatatcacgg cgacctctga cgtttccgaa cagccagtgt
    17461 ccggtccgct gtacatgaaa cgcctgaaag gcggcgtgct ggcaaacggc ctgaactcgt
    17521 ttgtttcggc ggtgtttaac accttcccga actcctgctt cgggcaaaac aacggagtga
    17581 tccagttgac cggtgttgcc agccgctatg tcggttttgt cgtcgcgctg atgttgatcg
    17641 tgctgggtct gttcccggca gtgagcggtt ttgtacaaca cattccagaa ccggttctgg
    17701 gcggcgcaac gcttgtaatg tttggcacca tcgccgcctc cggtgtgcgt atcgtttctc
    17761 gtgagccgct gaaccgtcgg gcgattctga ttatcgcgct gtcgctggcg gttggtctgg
    17821 gcgtgtctca gcagccgctg attttgcagt ttgcccctga atggctgaaa aacctgctct
    17881 cctccgggat cgccgcgggc ggtattactg ccatcgtgct gaatctgatt ttcccaccag
    17941 aaaaacagta attcactggc ggcggtaaga taattaccgc cgcagtctca cctctttaac
    18001 cattcccgcc ttgaggattg cgcgtaaatc gtgcataact cccttatgtg catttcacgg
    18061 gatggaagac catgaaattt attgggaagc tgcttctcta cattctcatc gctctgttag
    18121 tggcgatcgc tggcctctat tttcttctgc aaacccgctg gggagcagaa catatcagcg
    18181 catgggtttc cgagaatagc gactatcatc tggccttcgg ggcgatggtt caccgttttt
    18241 ccgcgccatc tcatatcgtg ctggagaatg tcacgtttgg tcgtgatggc cagcccgcga
    18301 ccctggtggc caaaagtgtc gacattgcgc taagtagtcg gcaactgacc gaaccacgcc
    18361 atgtcgatac catcctgctg gaaaacggga cgctgaatct caccgaccag accgcgccgc
    18421 taccgttcaa agccgatcgt ctgcaactgc gtgatatggc gtttaatagc ccgaatagcg
    18481 aatggaaact gagcgcgcag cgggtaaatg gcggcgtggt tccgtggtca ccagaagccg
    18541 gtaaagtgct gggtacgaag gcgcagattc agtttagtgc cggatcgctt tcgctcaatg
    18601 atgttcctgc caccaatgta ctgattgaag gcagtattga taacgatcgc gttacgctga
    18661 ctaacctggg tgccgacatc gcgcgcggga cattaaccgg aaacgcgcag cgtaacgccg
    18721 acggcagctg gcaagtggaa aacctgcgca tggcggatat ccgtctacaa agcgaaaaat
    18781 cgctaaccga cttctttgcg ccattacgct ctgtcccgtc gttgcagatt ggtcgcctgg
    18841 aagtgatcga tgctcgtttg caaggtccgg actgggcggt gaccgacctc gatctcagct
    18901 tgcgcaacat gaccttcagt aaagatgact ggcagacaca agaaggcaaa ctgtcgatga
    18961 acgctagcga gttcatttat ggttcgctgc atttatttga cccgattata aacgcggaat
    19021 tttctccgca gggcgtagcg ctgcgccagt tcaccagccg ctgggaaggg ggtatggtca
    19081 gaacgtcagg gaactggctg cgtgacggga aaacgttgat ccttgatgat gcggcaattg
    19141 ccggactgga atataccttg ccgaaaaact ggcaacagtt gtggatggaa acgacacccg
    19201 gttggttaaa cagcctgcaa ctgaagagat ttagcgccag ccgcaatctg atcattgata
    19261 tcgaccctga cttcccgtgg cagctcaccg cgctcgatgg ttacggtgcc aacctgacgc
    19321 tggttaccga tcataaatgg ggcgtctgga gtggctcggc gaatctgaat gccgccgccg
    19381 cgacgtttaa tcgtgttgat gtccgtcgtc cgtcgctggc gctaaccgcc aacagcagca
    19441 cggtgaatat cagcgaactg agtgcattta ctgaaaaagg cattctggaa gccaccgcca
    19501 gtgtttcaca aacgccacaa cgtcagacac atatcagcct gaatggacgc ggtgtgccgg
    19561 tgaatatttt gcaacagtgg ggatggcctg aattaccgtt gactggcgac ggcaatattc
    19621 agcttaccgc cagtggcgat attcaggcca atgtcccgtt gaaacctacg gttagcgggc
    19681 aattgcatgc cgtgaacgcc gcaaagcagc aagtgactca aaccatgaat gcgggcgtcg
    19741 tttccagcgg tgaagttaca tcggcagagc tggtgcagta aacatcaatc tcgccgggta
    19801 cgactcagac gtacccggca ttcttcaata gattacaacg taattgtcag cgcattccct
    19861 tgaggcgtaa ccaccagccc ctgctcactt tcagcctgcg aaccgccttg cagaccattt
    19921 actttcacaa tattgcgcaa gcacagtgtc cagttcctcg cctcgcccgc accagtcaca
    19981 gtaatcgtgt taccagtacg tgttgctttt agagtaaaga tcaccgaacc gtcagcagcg
    20041 ggcacttcac atacagcttc atgcccgtct tgcagattaa agagatggaa tgccgtgcct
    20101 tcgtgccacg cgtaatcggg acgttgatcg ttgttgccca gcgccaatag ggtgttatca
    20161 cgcacataaa cgggcagact caggaagccg tgctgctgtt tatgccagcg actaccgtcg
    20221 agctcattgt tgtgccacag gtgtgtccag cgaccttccg gcaagtagaa ctgcacctcg
    20281 cccgcctcag tgaacaccgg agcaaccatc acgttgtcgc ctagcatgta ttgacggtca
    20341 aggtaatcac aagccggatc gtccgggaac tccatcatca tggcccgcat catcggcgta
    20401 ccccgcgctt tcgcacgcgc agcttcacga tacagatacg gcatcatgcg gcatttcagt
    20461 tgcgtgaaga agcgcaccac atcacaggac tcatcatcgt aggcccacgg cacacgataa
    20521 gatttgctgc catgtaaacg actatggctg gagagcaaac caaacgcgca ccagcgtttg
    20581 taaacgtgtg ccggcgcggt attttcaaag ccgccgatat cgtggctcca gaagccaaaa
    20641 cctgaaaggc caatagacaa accaccgcgc aggctttccg ccattgattc gtagttagcg
    20701 taacaatcgc caccccagtg taccgggaat ttctgcgcac cgacggaggc cgagcgggca
    20761 aacaagacag cttcttcctc accaacggtg tccttgagca cgttccacac cagttcgttg
    20821 tagatgtacg cataatggtt atgcattttc tgcggatcgg aaccgtcaaa ccactgaaca
    20881 tcagttggga tacgttcgcc aaagtcggtc ttaaagcaat caacgcccat cgcgaccaga
    20941 cctttcagtt tgtcggcgta ccatttgcag gcatccggat tggtaaagtc ataaatcgcc
    21001 aga
//
LOCUS       DADMKF010000065        20827 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.066, whole genome shotgun sequence.
ACCESSION   DADMKF010000065 DADMKF010000000
VERSION     DADMKF010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20827
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.066"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            96..803
                     /locus_tag="JNC24_002195"
     CDS             96..803
                     /locus_tag="JNC24_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HAY9158936.1"
                     /translation="MDMGSQKILFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSS
                     WGIDDRDDLLRTIHRMIDNGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYSNWRQ
                     YFAGYTFGRQYWQSPEDDHLPLLREFLARKEYDDSGNDMFYQLFASDDAYYPALSWQP
                     LADYPTCPETLKDMSDL"
     gene            800..2227
                     /locus_tag="JNC24_002196"
     CDS             800..2227
                     /locus_tag="JNC24_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HAY9158937.1"
                     /translation="MKNCWKILEIEETTDVDIIRRAYLALLLSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMEEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSAGRLDIPELLDYARSWREAEPDNEDARYYEYAQRVYCGEGESLLAELCD
                     YWREYPSTQADALILQWCRQHRVDYYPLVVMMIEARELVNDQGKQLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGRKPRISLTRDTEHPLWPLYLVAKQLVQASQPT
                     EESLMPIVSRLDAEDRCPLEALIIRRLLIQAANFTGQETVEPEPQPQPMPVDDGGLGC
                     LGVIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(2237..2791)
                     /locus_tag="JNC24_002197"
     CDS             complement(2237..2791)
                     /locus_tag="JNC24_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HAY9158938.1"
                     /translation="MNKKSMYIFAIFIVAAITCISQPKKTTLRDKAMVNYAFDYLRSP
                     GSLPLTTAATELSAIHGHSTSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRA
                     QSKLGWIYLKGLGVKPDTRKAILWYKEAAEQGYAHAQYTLGLIYRNGTGINVNHYESQ
                     KWLKLAAKQHYKNAERLLAGLPAH"
     gene            2893..3600
                     /locus_tag="JNC24_002198"
     CDS             2893..3600
                     /locus_tag="JNC24_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HAY9158939.1"
                     /translation="MNKEEQYLLFALSAPMEILNQSCKPAHDSPKMYTGIKEFDLSSS
                     WGINNRDDLIQTIYQMTDDGHANDLAGLYLTWQRSSPEERKALIAGGSERGLIYTQFV
                     AQTAMCCGEGGIKAWDYVRMGFLSRVGVLNNWLTEEESLWLQSRVYARAHHYYHSWMH
                     YFSAYSLGRLYWQSSQCEDNASLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQP
                     LTVQPECPVTLKDVSDL"
     gene            3597..5048
                     /gene="djlC"
                     /locus_tag="JNC24_002199"
     CDS             3597..5048
                     /gene="djlC"
                     /locus_tag="JNC24_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HAY9158940.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVEEADDEEKDATAEHEILRAFRTLLDSESDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDSEGMDEEEREAFLEAI
                     QAGDCFDFLSLLEYPVALQNQTVEYYFALERCCRYHPDYVTAFLAMEGPWFIPDDAKL
                     HRKLLRWYSSVQTGMAELIPVAKQWQAEEPESEDARYYQCAQRLYCGEGESLLADLCA
                     YWESYPSTQADNLLLQWSKRHCPDYFALLVMVIEARSMVDAQGKPLKYVPGESARTRL
                     LWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEQDSPLLDLYRVAEQVVL
                     EAFPKQEMLARLNTRLEGGDAHPLEAIITQMLLTKVKLEPEDEDVDEPTPENHEEKND
                     EDEKPQSITSIIKISLTVLMIGYALGKIAMLFS"
     gene            complement(5108..6778)
                     /locus_tag="JNC24_002200"
     CDS             complement(5108..6778)
                     /locus_tag="JNC24_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
                     /protein_id="HAY9158941.1"
                     /translation="MDNAELAIGIDLGTTNSLIAVWKDGAAQLIPNKFGEYLTPSIIS
                     MDENNHILVGKPAVSRRTSHPDKTAALFKRAMGSNTNWRLGSDTFNAPELSSLVLRSL
                     KEDAEEFLQRPIKDVVISVPAYFSDEQRKHTRLAAELAGLNAVRLINEPTAAAMAYGL
                     HTQQNTRSLVFDLGGGTFDVTVLEYATPVIEVHASAGDNFLGGEDFTHMLVDDVLKRA
                     DVARTTLNESELAALYACVEAAKCSNQSPLHIRWQYQDETRECEFYENELEDLWLPLL
                     NRLRVPIEQALRDARLKPSQIDSLVLVGGASQMPLVQRIAVRLFGKLPYQSYDPSTIV
                     ALGAAIQAACRLRSEDIEEVILTDICPYSLGVEVNRQGVSGIFSPIIERNTTVPVSRV
                     ETYSTMHPEQDSITVNVYQGENHKVKNNILVESFDVPLKKTGAYQSIDIRFSYDINGL
                     LEVDVLLEDGSVKSRVINHSPVTLSAQQIEESRTRLSALKIYPRDMLINRTFKAKLEE
                     LWARALGDEREEIGRVITDFDAALQSNDMARVDEVRRRASDYLAIEIP"
     gene            complement(6870..7805)
                     /gene="rihA"
                     /locus_tag="JNC24_002201"
     CDS             complement(6870..7805)
                     /gene="rihA"
                     /locus_tag="JNC24_002201"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HAY9158942.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYSQGMTVV
                     DYYYLTGNKPNATVMVDVDRQGFVDLLADRLKFYA"
     gene            complement(7923..8648)
                     /gene="gltL"
                     /locus_tag="JNC24_002202"
     CDS             complement(7923..8648)
                     /gene="gltL"
                     /locus_tag="JNC24_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020457015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP binding
                     protein GltL"
                     /protein_id="HAY9158943.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMDPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            complement(8648..9322)
                     /gene="gltK"
                     /locus_tag="JNC24_002203"
     CDS             complement(8648..9322)
                     /gene="gltK"
                     /locus_tag="JNC24_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HAY9158944.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVAWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            complement(9322..10062)
                     /gene="gltJ"
                     /locus_tag="JNC24_002204"
     CDS             complement(9322..10062)
                     /gene="gltJ"
                     /locus_tag="JNC24_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HAY9158945.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWIWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQAYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAVIMLVMTLVERKVRLPGNMGGK"
     gene            complement(10232..11140)
                     /gene="gltI"
                     /locus_tag="JNC24_002205"
     CDS             complement(10232..11140)
                     /gene="gltI"
                     /locus_tag="JNC24_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HAY9158946.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKDKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHSDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWEIVGKPQSQEAYGCMLRKDDPQFKKLMDDTTAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            11595..13472
                     /locus_tag="JNC24_002206"
     CDS             11595..13472
                     /locus_tag="JNC24_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HAY9158947.1"
                     /translation="MSASSVKPLNVQLPAITLILFALCIGIFCYLAQWMSYEEVDQSA
                     LIHLGANVAPLTLSGEPWRLLSSIFLHSSVSHLLMNMFAFLVVGGVAEQILGKWRLLI
                     TWLFSGVFGGLISACYALRESEQIVISVGASGAILGIAGAAIATQFASGTGTYHKNQR
                     RVFPLLGMVALTLLYGARQTGIDNACHIGGLIAGGALGWLSARLSGQNRLVTEGGIIV
                     AGSLLLTGAIWLAQQQMDESVLQVRQSLREAFYPQEIEQERRQKKQQLAEERNALRET
                     LSAPVSREQASGDLLAEIADIHDMAISRDGNMLYAAIENTNSIVVFDLGQKKILHTFT
                     APIAKEKSVKHCGGCKDQGVRSLALSPDEKLIYATSFEANALSVINVATGEIIQSITT
                     GAHPDSLILSRDGTKAWVMNRTSNSVPAIDLVTYQHVADIPLEKYDGTGTSGKPGAWV
                     MALSPDERTLLVPGAGRGNIVRINTITHQKEDFPAGDARGTISAMRFRPENGEVIFAD
                     SQGISRISVGDQQASIMTQWCSRSVYSVEGISPDGQYLALVSYGLQGYVILLNINAGQ
                     IIGVYPASYVNHLRFSADGRKIFVMAKNGLIQMDRTRSLDPQAIIRHPQYGNVACIPE
                     P"
     gene            complement(13550..15088)
                     /gene="lnt"
                     /locus_tag="JNC24_002207"
     CDS             complement(13550..15088)
                     /gene="lnt"
                     /locus_tag="JNC24_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HAY9158948.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAAPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPIMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDGDQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSVNGIELTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEVQAMIPQFTREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     ARKR"
     gene            complement(15113..15991)
                     /gene="corC"
                     /locus_tag="JNC24_002208"
     CDS             complement(15113..15991)
                     /gene="corC"
                     /locus_tag="JNC24_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HAY9158949.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            complement(16081..16548)
                     /gene="ybeY"
                     /locus_tag="JNC24_002209"
     CDS             complement(16081..16548)
                     /gene="ybeY"
                     /locus_tag="JNC24_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HAY9158950.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            complement(16545..17624)
                     /locus_tag="JNC24_002210"
     CDS             complement(16545..17624)
                     /locus_tag="JNC24_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HAY9158951.1"
                     /translation="MFSFRRHTDKRNGLNIDTREITLEPADNARLLSLCGPFDDNIKQ
                     LERRLGIEINRRDNHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAI
                     KEARVLEQSAESVPEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTG
                     KTYLAVAAAVDALERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFE
                     MLGFEKVEKLIERNVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNS
                     KAVITGDVTQIDLPRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYE
                     AWEEAEQKRKAALAAERKREEQEQK"
     gene            complement(17738..19162)
                     /gene="miaB"
                     /locus_tag="JNC24_002211"
     CDS             complement(17738..19162)
                     /gene="miaB"
                     /locus_tag="JNC24_002211"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HAY9158952.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGSTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRV
                     AETPESVIARTRKENDLGVGYYQP"
     gene            19308..20483
                     /gene="ubiF"
                     /locus_tag="JNC24_002212"
     CDS             19308..20483
                     /gene="ubiF"
                     /locus_tag="JNC24_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol
                     hydroxylase"
                     /protein_id="HAY9158953.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPGSLIALHRDNDLQELELKGGETIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPARIRQLQNMNMAQFQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            complement(20637..20711)
                     /locus_tag="JNC24_002213"
     tRNA            complement(20637..20711)
                     /locus_tag="JNC24_002213"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20677..20679),aa:Gln,seq:ctg)
BASE COUNT         5106 a         5130 c         5361 g         5230 t
ORIGIN      
        1 attttatgcg tattatgttg cgtcagaaaa gtataaataa ctgagtctcg aaatgtagtt
       61 tacattcatt aaatgaatgt ttaaggataa attatatgga catggggtcg caaaaaatat
      121 tgtttgcact ttccactccg atggaaatac gcaatgaatg ctgtttacct tcgcattcat
      181 cccccaaaat gtatttaggt acatgctttt ttgacctttc ttcctcatgg ggaattgatg
      241 accgtgatga cctgctgcgc acaattcacc gaatgatcga taacggtcat gccgctcggt
      301 tggcagggtt ttatcaccgc tggtttcgtt attcgccatg tgaatggcgt gactatcttg
      361 ctgaactaaa tgagcaaggt caggcttatg cgcaatttgt cgccagcaca gccgaatgct
      421 gtggtgaggg aggaattaaa gcatgggatt atgtccggat gggtttttta agtcgaatgg
      481 gcgtacttaa taactggttg agtgaagaag aaagtctatg gatccagtcg cgtattcatc
      541 tacgggctct acgttattat agtaactggc ggcaatactt tgccggttat acctttggtc
      601 ggcaatattg gcagtctccc gaagatgatc atctgccttt attacgcgaa ttcttagctc
      661 gtaaagaata cgacgattcc ggcaatgata tgttttatca attatttgcc agtgatgatg
      721 cgtattaccc tgccttgtcc tggcaaccac tggctgacta ccccacatgc ccggaaacgc
      781 ttaaggatat gagcgaccta tgaaaaattg ctggaagatc ctcgaaatag aggaaacgac
      841 tgacgtcgat attatccgcc gcgcttatct ggcgctgtta ctgtcctttc atccagaaac
      901 cgatccgcag ggttttaaac aacttcgtca ggcgtatgag gaggcgctcc ggattgcgca
      961 gtcgcctgct aaatctgttt ggcaaccaga agaatatgag gtagcagaac atgaaattct
     1021 gctcgccttt cgcgcgttac ttgcctctga tagcgaacgt tttctgccct ccgcctggca
     1081 gcgattcatt cagcaattaa attattgctc gatggaagag attgatgaat tacgctggtc
     1141 gctgtgcaca atcgccatga acactgccca tttatccttc gagtgcgtgg tgttattagc
     1201 agaaagattg cggtggttgc aggaggaaaa cgtcggggaa atagacgaag aagaactgga
     1261 atccttttta tatgccattg cgaaggggaa tgtttttaac ttccagacca ttctgcatct
     1321 gcccgttgcc gtgcaaaatg acaccatcga tttttaccaa atgttcgctc ggatttggtc
     1381 atcgcatcca gaatggctga cattgtattt agcgcaacat cgcgcagtga tcatccccga
     1441 tgatgcaaaa ctgcacagaa atttactccg ctggtatagc gcaggtcgcc tggatatccc
     1501 cgaacttctg gattacgccc ggtcgtggcg ggaagctgaa cctgataatg aagatgcgcg
     1561 ttattatgaa tacgcgcaac gcgtctattg tggagaaggc gaaagcttgc tggcagaact
     1621 ttgtgactac tggcgcgagt atccctccac ccaggcggat gctttaatat tgcaatggtg
     1681 ccgtcagcat cgggtcgatt attacccgtt agtggtgatg atgattgaag ctcgtgaact
     1741 ggttaacgat cagggtaaac agctacttta tgtccccggc gacagcgccc gtacgcgttt
     1801 tcatttatac gaaatactca gcgatgaaaa actctctgcg ctggggcgtt cactggtcga
     1861 gatggttttg cacaaaggac gtaagccgcg gatctcactc acgcgtgata cagaacatcc
     1921 cttatggcca ttatatttag ttgccaaaca attagtgcag gccagccaac cgacagaaga
     1981 atcattaatg ccgattgtga gccgccttga tgcagaagat cgttgtccac tggaagcatt
     2041 aattattcgt cgattattaa ttcaggcggc gaattttacc ggtcaggaaa ccgtcgagcc
     2101 ggagccgcaa ccgcagccaa tgcccgttga cgatggcggg ctgggctgtc tgggcgtcat
     2161 caaaattatt ttctacattt ttatctttgc cggtttgata ggaaaaatac tccatctctt
     2221 cgggtgacag tcgacgttaa tgtgcgggaa gtccggcaag taatctttcc gcatttttgt
     2281 aatgttgttt ggcggccagt tttaaccatt tttgagattc ataatggtta acattaatac
     2341 cagtgccatt tctgtagatc aaacccaaag tatattgagc atgagcatac ccttgttcag
     2401 ctgcttcctt ataccagaga attgctttac gggtatcggg tttgactccc aggcctttca
     2461 ggtagatcca ccccagtttg ctttgtgcgc gcggattttc ctgttctgct gattgctcat
     2521 accagtatct cgcctgtgta taatccagtg gtttaccgtc gctaccatga agataaaatt
     2581 cgccaaggcg atattgcgat gttgagtgac catgaatcgc ggaaagctcc gtggctgccg
     2641 tggttaacgg tagactgcct ggtgatctta aataatcaaa ggcataattc accatggctt
     2701 tatcacgcaa cgtcgttttc ttgggttggc taatacaggt aattgctgca actataaaaa
     2761 ttgcgaatat atacattgat tttttattca tgtgtgaatg cttcgatttt ctttttattt
     2821 tgaaagcgtc aagtttatac tcatcaataa taaaaaagta gtcagttaat gcacttaagg
     2881 gattattaaa aaatgaataa ggaagaacaa tatcttcttt ttgcgctttc tgcgcccatg
     2941 gaaatactta atcagagttg caaacccgcg catgactcgc caaaaatgta tactgggata
     3001 aaagaattcg atctctcatc atcctgggga ataaataatc gtgatgattt aattcagact
     3061 atttatcaga tgactgatga tggtcatgcc aatgatttag ctggcttata tctgacatgg
     3121 caacgctctt cccctgaaga gaggaaagca ttaattgctg gtggctcgga gagagggttg
     3181 atttataccc aattcgttgc ccaaaccgct atgtgctgtg gagaaggggg aatcaaagcc
     3241 tgggattatg ttcgaatggg gtttctgtca cgggtcggtg tgctcaacaa ctggctgacg
     3301 gaagaagaga gtttatggct gcaatcgcgc gtctatgcac gagctcacca ttactatcac
     3361 agctggatgc actatttttc tgcctattca ctagggcgtc tctactggca atcttctcag
     3421 tgcgaagaca acgcttcgct acgcgaggcg ctaaccctgt acaaatacga tagcgccggg
     3481 agtcggatgt tcgaagagct ggcagcggga agcgaccgtt tctatgccac gcttccctgg
     3541 caaccattaa ctgtccagcc tgaatgcccg gtaacgctta aggatgtgag cgacctatga
     3601 aaacatgttg gcaaatcctc gaaattgaaa gcacgacgca aatagacatt atccgccagg
     3661 cttatcttgc tcgcttaccg ttgtgtcatc ccgaaaccga tccgcagggg tttaaagcat
     3721 tacgccaggc ctatgaagag gcgctgcgac tggcggtaaa tcctgtcgag gaagccgatg
     3781 atgaagaaaa agatgccacc gctgaacatg aaatactacg tgcattcagg acattactgg
     3841 attcagaaag tgatcgtttt cagccctccg cctggcagaa atttattcag caattaaata
     3901 cctggaacat ggaggatgtc gatcaattac gctggccgct gtgtgcaatc gctatagaag
     3961 cgcgatatct ttcattaaat tgtgcttctt tgttggcaga gcgtttgaac tggcattcgt
     4021 ttaatgacag cgaaggaatg gatgaggaag aaagggaggc ttttcttgag gccattcagg
     4081 ctggtgattg tttcgatttc cttagccttc tggaatatcc cgtcgcgtta cagaaccaga
     4141 ctgttgaata ttacttcgcg ctggaacgtt gctgccgtta ccatcctgac tatgtcactg
     4201 cgtttttggc gatggaagga ccgtggttca ttcctgatga tgcaaagtta catcgcaaac
     4261 tgttgcgctg gtacagctcg gtgcaaacag gtatggcgga actcattcct gtagccaaac
     4321 agtggcaagc ggaagaacca gaaagcgaag atgcccggta ttaccagtgt gcacaacgtt
     4381 tgtactgcgg cgagggggaa agcctgcttg ccgatctctg cgcgtactgg gaaagttacc
     4441 catctacaca agctgataat ttgttgttgc agtggagcaa gcgtcattgc ccggattatt
     4501 tcgcgttatt agtgatggtt atcgaagcgc gaagcatggt agatgcgcaa ggtaaaccgt
     4561 tgaaatatgt tcctggtgag agcgcccgga cgcggctgtt atgggcggag attttacata
     4621 gcggaaaatt atcgccgtta ggtcaatcgt ttattgagtc gttattcttc aagcgcaaag
     4681 catgggcgtg gtggaaatcg agagtcggta gcgagacaga gcaagattca ccgttactgg
     4741 atttgtatcg ggtagcggaa caggttgtgc tggaagcgtt cccgaaacaa gagatgctgg
     4801 cccgtcttaa tacaaggctg gaaggtggag atgctcatcc attagaggcc attatcaccc
     4861 agatgctgtt gacgaaagtg aaactcgagc cggaggatga agatgtcgat gagccaacac
     4921 ctgaaaacca tgaagaaaaa aatgatgagg atgaaaaacc acagagcatt accagtatta
     4981 tcaaaatcag cttaacggtg ctgatgatag gttatgcgct cggaaaaatc gcgatgttgt
     5041 ttagctgagg atgtgttttt tgcgccgatg ccggatgcag cgtttatgcc gcatccggca
     5101 gccgtgttta agggatctca atagccaaat aatcgctcgc ccgtcgacga acttcatcga
     5161 cgcgggccat atcgtttgac tgcaacgccg catcaaaatc ggtgatcacc cggccaatct
     5221 cttctcgctc gtcacccagc gcccgcgccc acaactcttc cagtttggcc ttaaaggtgc
     5281 ggttgattag catatcgcgc ggataaattt tcaatgcgga taaccgcgtc cgactctctt
     5341 caatctgctg cgcgctcaat gttaccgggc tatgattaat gactctggac ttaacgctgc
     5401 catcttccag aagcacgtca acttcaagca agccgttgat atcataacta aagcgaatat
     5461 caatcgactg ataagccccg gttttcttca acggcacatc gaaggattca accagaatgt
     5521 tatttttaac tttgtggttt tctccctgat agacgttaac cgtaatagaa tcctgttccg
     5581 ggtgcatggt cgagtaagtt tctacacgcg acacgggtac tgtggtgttt cgttcaataa
     5641 ttggcgagaa aatgccggaa acgccctggc ggttaacttc aacgcccaac gagtaagggc
     5701 aaatatcagt gaggatcacc tcttcaatat cttcactgcg taagcggcag gcggcctgga
     5761 ttgctgcgcc cagcgcgaca atggtgctcg ggtcgtaact ttgatacggt aatttgccaa
     5821 acagacgcac ggcgattcgc tgcaccagcg gcatttgtga cgcgccgcca accagcacca
     5881 gactgtcgat ttgactcggc ttcagacgtg catcgcgcaa cgcctgttca atcggcactc
     5941 gcaagcgatt gagcagcggc aaccacaaat cttccagttc gttctcgtaa aattcgcact
     6001 cccgcgtttc atcctggtac tgccagcgaa tgtgcaatgg cgattgattg ctacattttg
     6061 ccgcttccac acaggcgtac aaggctgcca gttcactctc gttaagcgtg gtcctggcga
     6121 catccgcgcg ctttaaaacg tcatcgacca acatatgggt aaaatcttcg ccaccgagga
     6181 agttatcgcc agcggaggcg tgaacttcaa tcaccggcgt ggcgtattcg agcaccgtga
     6241 cgtcaaacgt gccgccaccg agatcaaaaa ccagcgagcg ggtattttgt tgcgtatgca
     6301 ggccatatgc catcgcagct gctgtgggtt cattaattaa gcgtaccgca tttaacccgg
     6361 ctaactccgc tgctaaacgg gtatgcttgc gttgttcatc gctgaaataa gccggaacgg
     6421 agatcaccac atctttaatc ggacgttgca gaaattcttc ggcatcttct tttaatgagc
     6481 gtaataccaa agaggacagt tctggcgcgt taaatgtgtc gctgcctaac cgccagttgg
     6541 tattactgcc cattgcacgt ttaaataacg ctgccgtttt atccggatgc gaagtacgcc
     6601 gtgatacagc cggttttccg actaaaatat gattattttc atccatgcta attatggatg
     6661 gtgttaaata ttcaccgaac ttatttggaa ttaattgcgc ggcaccgtct ttccagacgg
     6721 caattaaact attggtagtg ccgagatcga taccaatggc gagttctgca ttatccattg
     6781 catttatatc ttgaaagaaa aagagataaa cagataaaca gattaagacc acccaggtaa
     6841 cgcgtggtga tcttaatcaa tgacgtgtgt taagcgtaaa atttcagacg atcagccagt
     6901 aaatcaacaa agccctgacg atcaacatcg accattacag tggcattcgg tttattgcca
     6961 gtcagataat aataatcaac aaccgtcata ccctggctat atttcccctg tgtttccacg
     7021 ccaacccagc gctcaacagt ggtaaacaac tcgggtttca acagccaggc gatggtgcat
     7081 gggtcatgca gcggtgcacc gacaaagccc catttttcgt ctttatgata ttcgaggaag
     7141 aaatccagca gttcggcaac aatggttgaa acagggttgc caatcgcgcg gaaacgctcg
     7201 gtgtcttcaa cgtggatttg tgctttgtga gtaacatcca gaccggccat caccactggg
     7261 atccctgact ggaagacaat ttctgccgct tccgggtcaa cgtaaatatt aaattcagcc
     7321 gcaggcgtcc agttaccaag ccccattgcg ccgcccataa tcacgatacg ggcaattttg
     7381 ctatgcagtt ccggatggct attgagcagc aaggcaacgt tagtttgcgg tccggtagac
     7441 acaatcgtga caggttccgc actttcgcgc agcgttttcg ccatcagctc taccgccgta
     7501 cagttttgcg gtgcgaacgt cggttccggt aatgccgggc catccagacc gctttcgccg
     7561 tgcacattgt ccgcgataat caactcacgc attaacggtt ttaccgcgcc gcctgctacc
     7621 ggaatatcgg tgcgattaag caaggtcagc atacgcagaa cattgcgtaa ggttttttct
     7681 ggtgtctggt ttccggcgga agacgtaatt gctttgacat caagctctgg tgaggcgagg
     7741 gcgagaacta ttgcgatagc gtcgtcatga cctgggtcgc aatctaacag aattggcagt
     7801 gccattgttg ctccttgttg tgtgcttctt tgcgacaagg gtaacgccag gatgtaacag
     7861 atacgagggg cgaaacgata aagcgtgaga tggcgcgcac ttgggtatgc gcgccagagt
     7921 gattaatgca ggattttcgc gaggaagtct ttcgcgcggt ccgatttcgg atcatcgaag
     7981 aaagcgtctt tcggcgagtc ttcgacaatt ttaccctcgt ccataaagat cacccgattc
     8041 gccactttgc gggcaaagcc catttcgtgg gtcaccacca tcatggtcat tccttcgttc
     8101 gccagttcca ccatcacgtc cagcacttcg ttgatcatct ccggatccag cgccgatgtc
     8161 ggttcgtcga acagcatcgc aataggatcc atacacaatg cgcgagcgat tgccacacgc
     8221 tgctgctgac cgccggaaag ctgcgccgga aatttattgg catgtgcgga aagtcccacg
     8281 cgctccagca atttcaatgc tttttcacga gcgggcgctt tatcgcgttt aagcactttc
     8341 acctgcgcca gggtcaggtt ttcgataatc gacagatgag ggaacagctc gaaatgctgg
     8401 aataccatcc cgacgcggga acgcagcttt gccagatcgg ttttcttgtc attaaccacg
     8461 ataccatcga cggtgatttc accttgctgc actggttcga ggccgttgac ggttttaatc
     8521 agcgttgatt tgccggaacc agacgggccg caaaccacca ccacttcgcc ttttttcact
     8581 tcggttgagc agtcggtcag cacctgaaag tgaccatacc attttgaaac atttttcagg
     8641 gtaatcatta tgctgtcctt cttttcaagt agctgaccaa caacgacgcg ctaagactaa
     8701 taacgaaata aacaaatccg gcaaacagga tcatctcaac ctgcgtacca tcacgctcac
     8761 caatggttga ggcggtacgg aagaaatcgg ccagagataa cacatacacc agtgaggtat
     8821 cctggaacag tacgatgccc tgagtgagca gaagcggcac catcgcgcgg aacgcctgcg
     8881 gcagaataat cagtttcatc gactgccagt gagtcattcc caacgccagc gcggcgctcg
     8941 actgaccacg agaaatactt tgaataccag cacggataat ctctgaatag taggccgctt
     9001 caaacatcga aaacgccacc atcgccgaaa ttaaacggat atcatttttt ggtgataatc
     9061 ccagcacgtt ttgcagaaaa cctggcacga tcaggtaaaa ccacagcaaa accataacta
     9121 aaggaatcga gcggaatacg ttaacgtagg ctttggcaaa ccacgctacg ggcgcaaagc
     9181 tggacaagcg catcaccgcc agcatggtgc cccacaaaat accaatcacg actgccgtga
     9241 cggtgatttt cagcgtgatc accagcccgt cgagcagata tggcagggaa gggacaatgg
     9301 aactccagtc aaactcgtac attatttgcc ccccatgttg ccaggcaggc gaactttacg
     9361 ttcaaccagc gtcattacca gcatgataac cgcgttaatc aacacatacg ccagcgtaat
     9421 agcggtaaac gactcccagg cgtgggctga gtaatcaagc aatttacccg cctgcgccgc
     9481 catatccacc agaccaatag tcgaggcgat ggcggagttt ttcaccaggt tcatcatctc
     9541 ggaagtcatc ggcgggacga taacgcgata agcattaggc agcagtacgt atcgataagc
     9601 ctgcggtagc gtcaggccca tcgccagcgc ggcatttttt tgcccacgcg gcaacgactg
     9661 aatggcggcg cgtacctgtt cgcaaacacg ggcggcggta aacagcccca ggcagagcat
     9721 ggatgaaagg aaaaactgaa tattgggatc cagctcggcc ttaaaccaca tgccgatttt
     9781 ctccggcaac agctccggga tcaccagata ccaggtaaag aattgcacaa tcaacggcac
     9841 gttgcggaac agttcgacat acaacgtacc cagaccggag agaaaacggt ttggaacggt
     9901 acgtaaaatg ccgaaaaatg aaccgacgag gaaagcgata atccaggcgc agatcgacaa
     9961 agcgatcgtc acctgaaaac cactccagat ccaaccaaga taggtggtgt tgccgaacgg
    10021 ggcttgttgt aaaaaaatac cccagttcca gtctatagac ataaatctac tccagaaaaa
    10081 agagggtagc agcgttaact gctaccctcg aagattgtta cccagcgtat tgcggttttc
    10141 aggcccgatg gggaacgacc atcaggcgta tagtctgtcc gtgctacgta acaatcgaga
    10201 gggctggaat ttccgcccct ggttcttgta attagttcag tgccttgtca ttcggttctt
    10261 tgaacagtgc tttcatttcg tctgacagtt cgaaattcat gttcaggttt ttcggcggaa
    10321 tgggattttt gaaccattta tcaaaccatt tttccgcttc accggaggtc tgcacctgcg
    10381 cggtggtgtc atccatcagc tttttgaact gcggatcgtc tttacgcaac atgcaaccgt
    10441 aggcttcctg agactgcggc ttgccgacga tttcccagtt gtctggtttc ttcgctttcg
    10501 cacgttcacc ggccagcagg gcatcatcca tcataaaggc aacggcacga ccgctttcca
    10561 gggtgcggaa agagtcactg tgatctttgg cgctgatgat gcgcatattc attttttgct
    10621 cttcattcag tttgttgagc aaaacttcag aggtagtgcc ggaagtgacg actacggctt
    10681 tgtctttcag gtcggcaaaa tctttgatat cgccgccctt tttggtcagc aggcgcgtac
    10741 cgaccacgaa aatagtatca gagaaagccg cctgtttttg gcgttcgacg ttgttggtgg
    10801 tagaaccaca ttcaaaatcg aaagtgccgt tttgcagcag tggaatacgg ttttgtgagg
    10861 taatcggaat cagttttacc tgcaagtccg gtttgttgag tttctttttc actgcttcaa
    10921 caatggcgtt ggagtaatcc tgcgagtaac ccaccacttt ttgctgattg tcgtaataag
    10981 agaaaggtac tgaagattca cggtgaccga cgacaatcac accgtttttg gcgattttgt
    11041 ccagcgtact gcctgctgcc ggggcggcgt catctgcctg tgccagtcct gcggaaagcg
    11101 ccagggcgag gattgctgtg gcaggtttac gtaattgcat atccaactcc tttatcttct
    11161 gcgttaagaa cgcatggata cccgttgtga gtgtttgtgt tgttatcgtc tgcaacttta
    11221 ttgtgcagtg ttgtgcctgt taggtaacat ttagtttggc taaatgtaaa gatattgctg
    11281 ttttattgtt tgtttttgcg agatgcgccg caccattccg aagcaaaatt cttaaaccgc
    11341 acctttttgg tgctaccgct gatttgtggt ggtgcaacct ggttgcgtcg cggctgattt
    11401 catcgcttac acagtaaata aagcaactta catgccaaaa aatacttagg ggcgaattaa
    11461 ttacgattgt aattttaggc tgaaaaagtc atatttcgga cagaattatc ttttcggcat
    11521 gggacgtttg ccgggatgat aactgcacac gggaaaaatg ctttttacct gtcttttcaa
    11581 ggaagaaaca ccttatgtct gcatcttcgg ttaagccgtt aaatgttcaa ttacccgcaa
    11641 taacccttat cctttttgca ctctgtattg ggatattttg ttatctcgca caatggatga
    11701 gttatgaaga ggtcgatcaa tccgcactta tccatctcgg cgcaaacgtt gcgcctctta
    11761 ctttgtcggg tgaaccctgg cgcttattga gcagtatctt tctgcacagt agtgtttctc
    11821 atctgctgat gaatatgttt gcattcctgg tcgtgggagg cgtggcggaa cagattctgg
    11881 ggaaatggcg actcctgatt acctggttat tctccggcgt ctttggtggg ctcatcagcg
    11941 cctgttatgc gttacgcgaa agtgagcaga tagtcatcag cgttggggca tccggggcga
    12001 ttctgggaat agctggtgca gcgatagcaa cacagtttgc ttcaggtacg ggcacatacc
    12061 ataaaaacca gcggcgagta tttcctctgt tgggtatggt ggcgctgaca ctgttgtacg
    12121 gtgcccggca aacaggaata gataacgctt gccacattgg cggcctgatt gcgggtggcg
    12181 cgttgggttg gctgagcgcg cgtttatctg ggcaaaaccg actcgttacg gaaggcggga
    12241 ttattgttgc gggcagtctt cttctgaccg gggctatctg gcttgcgcag cagcagatgg
    12301 atgagtcagt tttacaggtc aggcaaagcc tgcgtgaagc gttttatcca caggagattg
    12361 aacaagagcg acggcaaaaa aagcagcagt tagcggagga acgcaacgcc ctcagggaaa
    12421 cattatccgc tccggtaagt cgtgaacagg ccagtggtga tttgctcgct gagattgccg
    12481 atatccatga tatggcgatc agtcgggatg gtaatatgtt gtatgccgca attgaaaaca
    12541 cgaacagcat tgttgttttc gacctcggac aaaagaaaat cctgcatacc tttacagcgc
    12601 ccatagcgaa agaaaagtca gtcaaacatt gtggtggctg taaagatcag ggcgtcagat
    12661 cgctggcatt aagcccggat gaaaagttga tttatgcgac ttcatttgaa gcgaatgcgt
    12721 tatcggtcat taacgtggcg acaggggaga ttattcagtc gattaccacc ggtgcacatc
    12781 ctgacagcct tatcctctcg cgtgatggca caaaagcctg ggtgatgaat cgcaccagta
    12841 atagtgtgcc agcgattgat ctggtgactt atcagcatgt ggcggatatc ccgctggaga
    12901 aatacgacgg gacggggacg agcggtaaac caggcgcctg ggttatggca ctttccccgg
    12961 atgaaagaac attactggtt ccgggagcag gcagaggtaa catcgtgcgg atcaatacca
    13021 tcacgcatca gaaagaagac tttcccgcag gtgatgcgcg tggaacgata tcggcgatgc
    13081 gttttcgacc tgaaaacggc gaggttattt ttgccgatag tcaggggatt tcacgtataa
    13141 gcgtagggga tcaacaagcc agcattatga cgcaatggtg tagcaggagc gtttattccg
    13201 ttgagggtat tagcccggac ggtcaatatt tagcgttggt gtcatatggc ctgcaaggtt
    13261 atgtcatcct gctcaatatt aatgccgggc agattattgg cgtttatcct gccagctacg
    13321 ttaatcacct tcgtttttca gcggatggta gaaaaatatt tgtcatggcg aagaacgggt
    13381 tgatccaaat ggacaggacg cgctcgcttg atccgcaggc aattattcgt catccccaat
    13441 atggcaatgt ggcttgtatc cctgaaccgt aagaaaaagg ggcatgataa ccatgcccca
    13501 aagctcaatg agaaagtacg ttttgctcat gcaggataca tcaggcacat caacgtttac
    13561 gtgctcgcag actcatcaac acagcggcaa aaccaaacag cgccgtcagc acccacagtg
    13621 gccagttgcc ggtacgtgca tacggtgtga gtccggtggt cggtgtcaca ttagtggtca
    13681 acacctcgcg ggtgaactgc gggatcatcg cctgaacctc cccctgcggg ccaatcaccg
    13741 ccgtaatgcc gttgttggtg ctgcgcaaca gtgggcgcgc cagctccagc gcacgcattc
    13801 gcgccatctg gaagtgctgc catggaccaa tagacttacc aaaccacgca tcgttggaga
    13861 tagtcagcag atagtcggta tccgggcgga agttatcgcg cacttgctcg ccgagaatga
    13921 tctcgtagca aatagccgca gtaagctcaa taccatttac cgacagcggc ggctggatat
    13981 atggcccacg gctgaacgac gacatcggca gatcaaagaa cggtgctaac ggacgcagaa
    14041 tcgactccag cgggacaaac tcgccaaacg gcactagatg gtttttgtta tagcgatcgg
    14101 ctgattcgta gctgtatggt gcacctttac ccagtgtgat gatggtgttg taggtatcgt
    14161 agcggttctg cttattgaga cgcgcgtcga caatcccggt taccagcgag ctacctttat
    14221 cgcgcaactc accgtccagt gctttgagga acggttgctg attaatttcc agatcggtta
    14281 tcgccgactc cggccagata atcaacgatg atttgcccat cagcggagcc gttgcgttgt
    14341 agtaaatctt cagcgtatta agaagctgat ctccatccca tttcagtgat tgcggaatat
    14401 cgccctgaac catcgaaacc tgaatggttt tctccggttg cggggtaaac cattggatgt
    14461 aacgcagcgg gaagggtagg gcaaacagca cgacggccac caccagcgga cgccagttgc
    14521 gtttgaccaa cgccagtgcc agcaggccac taaccatcat cagcaggaag ttaatggctt
    14581 ccacgcccat tatcggtgcc agccctttta acgggccatc aatctggcta tagccgaact
    14641 gtaaccacgg gaagcctgtc agtacccaac cgcgcagaaa ctcggtcact tgccagagag
    14701 caggggcggc aatcgccacg cgcagccagg tggttttcgg ccacagacgc gacagcacgc
    14761 cagcaaacag tccggtatac agcgacaaat acgccgccag cagcaccacc aggaagatgt
    14821 taaccgggcc aggcattccg ccaaaggtcg cgatgctgac atagacccag ttaataccgc
    14881 tgccaaagag gccaaatccc cagcaaaagc caatagcggc agactggagt ggacggcggt
    14941 taaaggtcaa cgcctgaagc cccatcaacg aaataatcgc cgcaggccag acgtcgtaag
    15001 gagagaaggc cagcgttccg caggcaccga ataataacgc cagcagcagg cgaatgcgct
    15061 ggcgttcaat taatgaggca aaagccatgt agttatctat ccagtttcgg ttttattcat
    15121 ccagcttcgg ctggggtgag tcatccggga ttttgacatg aacctgaata atacgccgac
    15181 tgtcggccat cgccactttg aactggtaac cgtcgatgtc gatagtttcg ccacgcgccg
    15241 gaagatgccc aaatgcctgc atcaccagac caccgatagt gtcaacctct tcatcgctaa
    15301 agtgggtgcc gaacgcttcg ttgaagtctt caatggaagc cagtgcacgc acggtccagg
    15361 tatgacgact cagctgacgg aagtcgatat catcttcttc gtcatactcg tcttcaatct
    15421 caccaacaat cagttccagg atgtcttcaa tggtcaccag accggaaacc ccaccgaatt
    15481 cgtcaatgac gatcgccatg tggtaacgct gagagcgaaa ctctttcagc atccggtcta
    15541 cgcgcttact ttcaggaacg acaaccgcct gacgtaacac tttgtccatg ctgaaggctt
    15601 cagcatcgct gcgcataaac ggcagcaagt ctttcgccat cagaatccct tcaatgtgat
    15661 ctttgtcttc gctaatcacc gggaaacgtg agtgggcgga ctcgatgatg acatcaaggc
    15721 attcgtccag cgtctggttg cgtttcaggg taatcatctg ggagcggggg atcatgatgt
    15781 cgcgaacgcg ttggtctgcg atgtccatca ccccttcgag catatcgcgc gtatcttcgt
    15841 cgataaggtc gttctgcccg gaatcacgga tcagcgccag cagttcgtca cggtttttcg
    15901 gttcaccgtg gaaaagttgg ctgagtaaca gggagaaaaa tcccttcttg ttgcttatcg
    15961 tgtcactact gtgtgaattg tcgtcgctca tggcgtcgta tgggttctca tgttagttaa
    16021 tcaaaacgtc gtcgttaatc accaacggcg gggacgtctg ccagtcaaat gcctggcaaa
    16081 ttattctttc tcggcaatgt acggatcctc atagcccaga gcaagcataa tctctgtttc
    16141 gagggcttcc atttcttctg cttcgtcatc ttcgatgtga tcgtaaccta acaaatgcag
    16201 actgccgtgc accaccatat gcgcccagtg cgcctccagt ggtttgcctt gctcctgagc
    16261 ttccttctca accacctgac ggcagataac cagatcgccc agtagcgaca tttccatgcc
    16321 aggcggcact tcaaacggga aggagagcac gttggtcggc ttatccttac cgcgataggt
    16381 cagattcaga ctgtggcttt cggcggtatc gaccacgcga atagtcactt ccgattcttc
    16441 ctgaaactgc gggatcaccg cattcagcca tgtttgaaac tggctctctt ccggtaaccc
    16501 ggaattatct tcacatgcca gttgtaaatc gaggatcacc tgactcattt ttgttcctgt
    16561 tcttcgcgct tgcgttctgc cgccagcgcc gcttttcgtt tttgttcggc ttcttcccag
    16621 gcttcatagg cgttaacgat acgcgccacc acagggtgac gaaccacgtc ttcgctgtgg
    16681 aagaagttaa agctgatctc ttcgacatcg gccagcactt cgatggcgtg acgtaagcct
    16741 gatttagtat tacgcggcag gtcgatctgt gtgacgtcgc cggtgataac cgcttttgag
    16801 ttaaaaccga tacgggtcag gaacatcttc atctgttcga tggtggtgtt ctggctctca
    16861 tcgagaatga taaacgcgtc gttcagcgta cgaccacgca tataggccag cggcgcgact
    16921 tcaataacgt tgcgctcaat cagtttctca actttctcaa agcccagcat ttcaaacagc
    16981 gcgtcgtaca gtgggcgcag atacgggtct actttctggc ttaaatcgcc aggcaggaag
    17041 cccagtttct caccggcttc gaccgccgga cgagtcagca gaatacggcg aatctcctga
    17101 cgctccaggg catcaactgc cgcagccact gccaggtagg ttttacccgt acccgccggg
    17161 ccaacgccga aggtaatgtc atggtcgaga atattggcga tgtactgcgc ctggtttggc
    17221 gtgcgcggct taattacgcc gcgtttggtt ttgatattga ccgctttgcc gtactccggc
    17281 acgctctccg cactttgctc cagtactcgc gcttctttaa tcgcaaggtg gatctgttcc
    17341 ggttcgatat cctgaatctg accgcgcatc ggggctgtat cgacatacag gctacgcaga
    17401 atgtctgccg cagcggtgac gcaaatcgga cggccggtca gtttaaagtg gttgtcgcgg
    17461 cgattgatct cgatgccgag acggcgttcg agctgcttga tgttgtcatc aaacgggccg
    17521 cacaggctca acagacgcgc gttgtctgct ggctccaggg tgatttcgcg agtgtctatg
    17581 ttcaaaccgt tcctcttatc tgtatgccgc cggaagctga acattcaccg gcctataagg
    17641 aaattattca cgccacagga aaaaggcgca agcattgcga taaagatggg gataaagaga
    17701 gaaaaaacaa ggcccaccgg aacggcgggc ctgagaatta cggctgataa taacccacgc
    17761 caaggtcgtt ttctttgcgg gtacgggcaa tcaccgattc cggtgtttct gccacgcgca
    17821 gacccatttc atcttcagta cgcaccactt taccgcgcag agagttcggg tagacgtcgg
    17881 taatttctac atcgacgaat ttaccgatca tatccggtgt gccctcgaag ttgaccacgc
    17941 ggttattttc ggtacgcccg gaaagctcca tgatgctctt acgcgaagtc ccttctacca
    18001 gaatacgctg ggtggtgccg agcatccggc ggctccatgc catcgcttgc tgattgatac
    18061 gttcttgcag aatatacaga cgttgcttct tctcttcttc cggaacatca tcaaccatat
    18121 cggcggctgg tgtacccgga cgtgcagaga agataaagct gtagctcatg tcgaaattga
    18181 cgtcggcaat cagcttcatc gttttctcga agtcttcggt ggtttcgcca gggaagccaa
    18241 caatgaagtc agaactgatc tgaatatccg gacgcgccgc acgcagttta cggatgatcg
    18301 ctttgtactc cagcgccgtg tgagtacggc ccatcaggtt cagaatgcga tcggaaccgc
    18361 tttgtactgg cagatgcagg aagctcacca gctccggcgt gtcgcgatac acttcgatga
    18421 tatcgtcggt gaattcgatc ggatggctgg tggtaaagcg aatacgatcg atcccgtcga
    18481 tcgcagcaac cagacgcagc agatcggcaa acgatccggt ggagccgtcg tagttttcac
    18541 cacgccaggc gttcacgttc tgaccgagca ggttgacttc acgcacgccc tgagccgcaa
    18601 gctgggcaat ctcaaacaga atatcgtcag acggacggct tacctcttca ccacgggtgt
    18661 aaggcaccac gcagtaggtg caatatttat tgcagccttc catgatggag acaaacgcgg
    18721 tcggcccttc ggcgcgcggt tccggtagac ggtcaaactt ctcgatttcc gggaagctga
    18781 tatctacaac cgggctgcgg tcgccgcgca cggagttgat catctccggc agacggtgca
    18841 gcgtttgcgg cccaaaaata atatcgacat agtgggcgcg ctggcgaatg tgctcgcctt
    18901 cttgcgatgc cacgcagcca ccgacgccga taatcaggtc tggattcttc tcttttaaca
    18961 gtttccagcg acccaactga tggaagactt tttcctgagc cttctcgcgg attgagcagg
    19021 tgttcagcag cagcacatcc gcttcttccg ccacgtcggt cagttgatag ccgtgggtgg
    19081 catccagcag atcggccatc ttcgatgaat cgtactcgtt catctgacag ccccaggttt
    19141 taatatggag ttttttggtc atcgacttgc tcttgcgaaa tagtagccag gaatgcaggg
    19201 cgtatagtgt aatgctttgc tgccgttgtg accagtatga gcgttatcag cccttggggg
    19261 taaaaatcct gtaaacttaa agcatattgc taacaggatg attgaccatg acaaatcaac
    19321 caacggaaat tgccattgtc ggcggaggaa tggtcggcgg cgcgctggcg ctggggctgg
    19381 cacagcacgg attttcggta acggtgatcg agcacgcaga acctgcaccg tttgtcgctg
    19441 atagccaacc ggacgtgcgg atctcggcga tcagcgccgc atcggtatca ttgcttaaag
    19501 ggttaggggt ctgggatgca gtacaggcta tgcgttgcca tccttaccgc agactggaaa
    19561 cgtgggagtg ggaaacggcg catgtggtgt ttgacgccgc tgaacttaag ctaccgttgc
    19621 ttggctatat ggtggaaaac accgtcctgc aacaggcgct gtggcaggcg ctggaagcgc
    19681 atccgaaagt aacgttacgt gtgccaggct cgctgattgc attacatcga gataatgatc
    19741 ttcaggagct ggaactgaag ggcggtgaaa cgatccgcgc gaaactggtg attggtgccg
    19801 acggcgcaaa ttctcaggtg cggcagatgg cgggaattgg cgttcatgcc tggcagtatg
    19861 cgcagtcgtg catgttgatt agcgtacagt gtgagaacga tcccggcgac agcacctggc
    19921 agcaatttac cccggacgga ccgcgtgcgt ttctgccgtt gtttgataac tgggcatcgc
    19981 tggtgtggta tgactctccg gcgcgtattc gccagttgca gaatatgaat atggcgcaat
    20041 tccaggcgga aatcgcgaag catttcccgt cgcgtctggg ttacgttaca ccgcttgccg
    20101 ctggtgcgtt tccgctgacg cgccgacatg cgttgcagta tgtgcagccg gggcttgcgc
    20161 tggtgggcga tgccgcgcat accatccatc cgctggcggg gcagggggtg aatcttggtt
    20221 atcgtgatgt cgatgccctg attgatgttc tggtgaatgc ccgcagctac ggtgaagcgt
    20281 gggccagtta tcctgtcctc aagcgttacc agatgcggcg catggcggat aacttcatta
    20341 tgcaaagtgg tatggatctg ttttatgccg gattcagtaa taatctgccg ccgctgcgtt
    20401 ttgtgcgtaa tctcgggtta atggcggcag agcgtgctgg cgtgttgaaa cgtcaggcgc
    20461 tgaaatatgc gttagggttg tagccttaca acattgccgg atgcgtgcca accgtaggtc
    20521 ggataagacg cggcagcgtc gcatccgaca ttgaaggata agacgtgtca acatcgcatt
    20581 cgacattgaa tgaacgcaga aaagcaaaaa gctcgccgaa gcgagctttt ttaatgtggc
    20641 tggggtacga ggattcgaac ctcggaatgc cggaatcaga atccggtgcc ttaccgcttg
    20701 gcgatacccc aacaaattgg tttttgaatt tgccgaacgt attcgacaca ttcagaattt
    20761 ggtggctacg acgggattcg aacctgtgac cccatcatta tgagtgatgt gctctaacca
    20821 actgagc
//
LOCUS       DADMKF010000066        20711 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.067, whole genome shotgun sequence.
ACCESSION   DADMKF010000066 DADMKF010000000
VERSION     DADMKF010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20711
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.067"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(73..4533)
                     /gene="mukB"
                     /locus_tag="JNC24_002214"
     CDS             complement(73..4533)
                     /gene="mukB"
                     /locus_tag="JNC24_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HAY9158954.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTAQRQQEKIERYEADLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     EQEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFTS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNVVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLPGSDEAPSQES"
     gene            complement(4533..5237)
                     /gene="mukE"
                     /locus_tag="JNC24_002215"
     CDS             complement(4533..5237)
                     /gene="mukE"
                     /locus_tag="JNC24_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HAY9158955.1"
                     /translation="MSSTNIEQVMSVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEENQPDSGEEE"
     gene            complement(5218..6540)
                     /gene="mukF"
                     /locus_tag="JNC24_002216"
     CDS             complement(5218..6540)
                     /gene="mukF"
                     /locus_tag="JNC24_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HAY9158956.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            complement(6537..7322)
                     /gene="cmoM"
                     /locus_tag="JNC24_002217"
     CDS             complement(6537..7322)
                     /gene="cmoM"
                     /locus_tag="JNC24_002217"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HAY9158957.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            7458..8237
                     /gene="elyC"
                     /locus_tag="JNC24_002218"
     CDS             7458..8237
                     /gene="elyC"
                     /locus_tag="JNC24_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HAY9158958.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTAKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEELNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGPSGEPRQE"
     gene            complement(8214..9107)
                     /locus_tag="JNC24_002219"
     CDS             complement(8214..9107)
                     /locus_tag="JNC24_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HAY9158959.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(9261..10007)
                     /gene="kdsB"
                     /locus_tag="JNC24_002220"
     CDS             complement(9261..10007)
                     /gene="kdsB"
                     /locus_tag="JNC24_002220"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HAY9158960.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQASPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            complement(10004..10186)
                     /locus_tag="JNC24_002221"
     CDS             complement(10004..10186)
                     /locus_tag="JNC24_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HAY9158961.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            complement(10238..11470)
                     /locus_tag="JNC24_002222"
     CDS             complement(10238..11470)
                     /locus_tag="JNC24_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HAY9158962.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTMLQKGLRQAITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            complement(11507..12493)
                     /gene="lpxK"
                     /locus_tag="JNC24_002223"
     CDS             complement(11507..12493)
                     /gene="lpxK"
                     /locus_tag="JNC24_002223"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HAY9158963.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTSLASGN"
     gene            complement(12490..14238)
                     /gene="msbA"
                     /locus_tag="JNC24_002224"
     CDS             complement(12490..14238)
                     /gene="msbA"
                     /locus_tag="JNC24_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HAY9158964.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DNLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAIDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            complement(14275..16539)
                     /locus_tag="JNC24_002225"
     CDS             complement(14275..16539)
                     /locus_tag="JNC24_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309023.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
                     /protein_id="HAY9158965.1"
                     /translation="MKITTVDVCIICGIFPLLILPQLPGTLALAFLTLFACVLAFIPV
                     KTVRYIALTLLFFVWGILSAKQILWAGETLTGATQDAIVEITATDGMTTHYGQITYLQ
                     GRRIFPAPGLVLYGEYLPQAVCAGQLWSMKLKVRAVHGQLNDGGFDSQRYAIAQHQPL
                     TGRFLQASLIEPNCSLRAQYLASLQTTLQPYPWNAVILGLGMGERLSVPKEIKNIMRD
                     TGTAHLMAISGLHIAFAVLLAAGLIRSGQIFLPGRWIHWQIPLIGGICCAAFYAWLTG
                     MQPPALRTVVALATWGMLKLSGRQWSGWDVWICCLAAILLMDPVAILSQSLWLSAAAV
                     AALIFWYQWFPCPEWQLPPVLRAVVSLIHLQLGITLLLMPVQIVIFHGISLTSFIANL
                     LAIPLVTFITVPLILAAMVVHLSGPLILEQGLWFLADRSLALLFWGLKSLPEGWINIA
                     ERWQWLSFSPWFLLVVWRLNAWRTLPAMCVAVGLLMCWPLWQKPRPDEWQVYMLDVGQ
                     GLAMVIARNGKAILYDTGLAWPEGDSGQQLIIPWLHWHNLEPEGVILSHEHLDHRGGL
                     DSILHTWPMLWIRSPLNWEHHQPCVRGEAWQWQGLRFSVHWPLQASNDKGNNHSCVVK
                     VDDGTNSILLTGDIEVPAEQKMLSRYWQQVQTTLLQVPHHGSNTSSSLPLIQRVNGKV
                     ALASASRYNAWRLPSSKVKHRYQQQGYKWLDTPHQGQIAVNFSAQGWRISSLREQILP
                     RWYHQWFGVPVDNG"
     gene            complement(16746..17030)
                     /gene="ihfB"
                     /locus_tag="JNC24_002226"
     CDS             complement(16746..17030)
                     /gene="ihfB"
                     /locus_tag="JNC24_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HAY9158966.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            complement(17190..18863)
                     /gene="rpsA"
                     /locus_tag="JNC24_002227"
     CDS             complement(17190..18863)
                     /gene="rpsA"
                     /locus_tag="JNC24_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HAY9158967.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(18974..19657)
                     /gene="cmk"
                     /locus_tag="JNC24_002228"
     CDS             complement(18974..19657)
                     /gene="cmk"
                     /locus_tag="JNC24_002228"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HAY9158968.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPASDALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            complement(19830..20594)
                     /locus_tag="JNC24_002229"
     CDS             complement(19830..20594)
                     /locus_tag="JNC24_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HAY9158969.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
BASE COUNT         4970 a         5732 c         5045 g         4964 t
ORIGIN      
        1 aatgtgccac ttttttagtg cagaaacgga gttttcggaa aaagaaaagg cggcattgct
       61 gccgccttaa ttttaactct cctgagaagg cgcttcgtca cttcctggaa gcgtttcagg
      121 gagttgcggc gcaaatcctc gcaggccgac gacgtgaacg tgttcggtat tctggaagac
      181 tttacgcacc agtttatagg tggtgccttt ctccgggctg atattttccg gcgctgcgat
      241 gatgagctgc atttgcaaac gttcacacaa ttcaaacagc gtggcgatag aacgagcatc
      301 cagtcgcgct gcttcatcga ggaacagcag gcggcaagga gagatatctt taccgcgcag
      361 gcggcgagat tcatcttccc agctttgtac caccatcacc agaatcgaca taccggtacc
      421 aatcgcctca ccggtcgaca atgcaccaga ctctgcgcgc aaccagccat cggaaccacg
      481 gttaacctca acttccattt ccagatagtt gcggtaatcc agcagttctt cgccaatggt
      541 ctgcggcgtg cgctgcccca tatcaatctg cggattaaga cgctgataca atttcgccag
      601 cgcttccgag aaggtcagac ggttgctgtt aaacagatcc tgatgctgct cgtgctgttc
      661 agagagcaca tccagcagca tggcgtgcgt ttcacgcacg ttcacgttca gacgtacgct
      721 gttcacctga ccaaacgata cgttctgcaa cccctggttg agcatacgga tacggttctg
      781 ttcgcgctga atggttttgc gaatgatgtt cgccacgctg cgggaactga tcgccagttt
      841 ctgttcgcgg gaggttaact cctcggtcag tcggctaagt tcgatttcca tctgctcgat
      901 ggcttccacc ggatcatcgg tacgaataat atcctgacga atacgttcac gcagatgctg
      961 ataaaccgcc acgaagaact gaattttacg ttccggacgt tttggatctt ccgacatgcg
     1021 cagcacgtcg cgcagatgtt cgttatccgc caccgccaga cgcagcgcac ccaatgcctt
     1081 atccgacata gagcgcaaat catcagcgga gagataagcc agctcgcgac ggtgtaagcg
     1141 ccgttcaacg acgttatctt tcaccatgcg catcactgca caccagcccg ctttggcggt
     1201 cactacctgc tcgcgcatct caaagtaatc ccgctccagc ttgcgcagtt tgcgggtcag
     1261 attgtccatc tccgcttcgc agaaagtcag tgctttttcc agctggttgc ggcgggaccg
     1321 gttattactc agttgcgcat gcagctcgtc acggcgaatt cgcgcccgct cttctgcccc
     1381 gctatcggcg cgcacgccga tatcctgcaa ttcacgttgc agatcgttga gtagctcttt
     1441 tttggtgtcg taagaacttt tcagcgaagc cagcacctgg ttgtactgac tcaactgcgc
     1501 tgcgtgacca cgcaacgctt cacgagcgcg agtacgctcc gcttccgcct gttccagacg
     1561 ttcacgcagc ttttcgttga gatcgctatt accgctcagc atttctgccg agtcagaata
     1621 gctaaagtgc gcacgacgct gcaccacttc cgtcagggca aacgcctgct gacgggcatc
     1681 gcgctgcatc tgctgagagt acgcgtaatc ttcttttagc tgttcgaact gttccgggtc
     1741 gctctgcaat accgaaacga tcggttccag tttcgccagt tgattgccaa actgctgaac
     1801 aaaacgcgcg gcttcctggg cttcatccag acgttcgcgg atttcatcga cgcgatccgc
     1861 caggctgtca tcagccaaca ggttgagacg cggcagaatg cggttcagcg ccgtaacgcc
     1921 ttctttcgcc tgctcaaact gaatacgctg ctgctggtta tcattttcat gattactcaa
     1981 cgcccgctcc agttcgacgc gacggctgtt cagttgacgg atttctgctt ccgggtcaga
     2041 ctcaaacgcc accgccagat gactgccgat aaagcggctg aacgcctgat gcagacgctg
     2101 ggttttctgt acatcaaagg agagcgtggc gaagcgttcg gaaagcactt cacgctcggc
     2161 atggaggctt tcaatacggc tttcacgcgc agcacgacca aacagcggca cttccgggaa
     2221 acgtgaataa cgccactgac gatcggcgat tttcaccact accgcttttt ccagctcatc
     2281 aacgctgaac acgctgtcat cgaatgactg cggatcccct tcgatcaaat agagatcttc
     2341 cgggcaatcg gtcaagcctt ccaggtgttc agttacctgt gacagatctg gcaccacgat
     2401 ggcgtggcgt gacgggccat acagcgctga gaagtacggc gcatcttcca ggctaacgtc
     2461 gtcataaatt tctgacagca gcacgccgcc aaaacgctcc gccagcgcgt tcagacgctg
     2521 atcttcagag ccgccaggct ggcttaaacg ttcgatctct tcatcgacag cgtttttgcg
     2581 cgcgcccact tcatcgcgtt caacaatcgc ctctcgctca cgctccagca actgttgcag
     2641 atattcggtg acgtcctggc tggaggtaaa ctcttcaccg cactgctcgc tcaactggtt
     2701 gagactgttt tgcgctgcca gccaaaccgg cgcacgctgc atcaaactct gaatgcgaga
     2761 ctgcagctgt tcctgttcct ggcgcagtgc catgcgctct tcacgggcgt tagacacgct
     2821 atcggaaaga gaggcaatgc gcgcttccag ctcctgatgc agagcttcca gttcatcaat
     2881 atcaaaattc ttgccctgac gtttgcagaa gtctgccagc aggcgttcgg cttcttgctg
     2941 ctcgcgcaga cgctgttcca gttcacttaa gcgcatccgc aacggttgaa cctgctctgc
     3001 caggtgacgc tgatcgaccc cttcgcgcag cagttcgcgg gcgacatccc aagcctcgtt
     3061 acgcgccagc gggccgttga ttgccaccac cagctgataa gcctgctcaa actggctgtg
     3121 cgcggtttgc gccatgctca ttttctgctc aagagagagc attttttcag tcgcttcctg
     3181 ctctttcgcc tggaaggttt ccagccattc ggcggcgctg tcggcggtta agtccggcag
     3241 atggcacagt tctttggcgc gattaagcgc agcaatcgcc tggttatact ggatcgcgcg
     3301 cgtttgctgg acgtccagcg cctgctggta gtcagcaagc tggcttttca gctcgtccac
     3361 ttccagttcg gcagcttccg cgcgagcctc gttctcttcc tggcgttcga tggcttctgc
     3421 caccacttcg ttttgctctt ccagacggat ctgcagctca tcgagatccg cttcgtagcg
     3481 ttcgatcttc tcctgctgac gctgtgcggt ttgcaccagg ttcaggtgat cgctggccgc
     3541 ctgataatcc gcttccagat cgccttcggc accgttgtgc tctgccagct cacgcgccat
     3601 atcgacgtgt ttgtactgct cagccgccag ttgctgacgc gaagtatgta actcgcgacg
     3661 aaactccagg gctttgtcga gatggacacg gcgctcgttg gcgtgacgca tgtagtccgc
     3721 cgccacgtag ttggtagctt cgctgatcag atgcttaaac aggtcacggt ccgactgggt
     3781 gacacgaatc gcttccagcg tcatgcggtt ttcacgcagc gccgcttcca tgtcctggaa
     3841 cgctttacgc acgccgctgt tttccggcaa caggtagtcg cgcagagaac gggtaatggc
     3901 actggagatc ccgccataca gcgaagcttc gatcagacga tagaatttgc tacggtcaga
     3961 tgcggagcgc agacgacgcg cgatgatgcc cagatcgaac atcagcgagt ggtaatcggt
     4021 aatggagttg aactgtttaa actgcacgcc ttccatcgcc tcgagcttgt ctttcagctc
     4081 gttaagcggc agcacgcgcg cctggcgttc gttcagggtt tcggtcacca gctgtgtcgg
     4141 ctgcaccgac atcggcagtc cctgaatggc aaatggtttg atatccactt tacgatcgcg
     4201 tccggcaacc tgttgcagac gcacaccgac caccacgcgc tggtggcgcg agttaatggt
     4261 gtcgagcatc gaataacaga cacccgcttt cagcttaccg tgcagacctt tatcgcgcga
     4321 accgctggtg gccccggctt ccgtagtgtt acggaaatgc agcagggtca ggtcggggat
     4381 cagcgccgta acgaacgccg ccatggtggt ggatttaccc gccccgttac cgccagaaag
     4441 cgtcgtgacc agctcgtcaa ggtcaaaagt tcgggcaaaa aagccgttcc agttaatcag
     4501 cgtcagtgag cgaaatttac cgcgttcaat cattattctt cctctccgct atctggctga
     4561 ttctcttcgg tttcatcgtt gagttgcaga tgattttcaa tcggcattgc ttcgccatca
     4621 cgaatcaggc gacgctgtgc ttcacgggga tcgtcgccag cacgcacatc ggctccgaag
     4681 cggaacaccg attcggtaat gcgaaacttg ctgctgtcgt ggcccataaa ccacaccatg
     4741 cctaaacgac gcagacggtt gagcgaagaa cgtactttct cctgcaactt ctgacggtca
     4801 acgtctgaac cggttgaacg gttgttcacc agtttcagca gttttgcttc atcggccagg
     4861 gtgagcagtt cgtcgtacag ttcctgctgg gtgaaaatcc cctcattcgc cagccgttcc
     4921 gggctgagat agagataaca gaggattttc ccgaccatca tatccagttc cgacaagacg
     4981 gaacgcggga taagagtggt ggaacgtggg cgtaaataga agaacccttc cggtgcgcga
     5041 ataagctcaa cgttataacg cgcgtaaaac tcttccagat attcctgaaa atccatcagg
     5101 aatgcatgat tatccagttc gtcgaggcca atatggcggc ctgaacgtaa ggcgctgtcc
     5161 agcgccggaa ataacggatt cgccagcgcc tgcgccagct taaccgacat cacttgttca
     5221 atatttgtcg atgacatgcg cctgtacctt ggctccgtaa tcattaatcg gctgccattt
     5281 cgctggcagt ccggtgaaat ctgcttgcgc tacgccaaga cgtaccgcct gatcaataac
     5341 aatacgcgca acgtcaaagt gacgtgcacg cggatactgt gacagatatt cgcgtaccac
     5401 cagaccaaga tccagcggca cttgtctggt tttgtacacg gcaagttgtt cttcgatgat
     5461 cgccgccagc tgttcgcgga tctcgttaaa ctcttcgtat tccagatcct caggaagttc
     5521 cccagtcact tcttcatcgc gcagtgccat ctcttcgtca cgcatatcca gcagacgatc
     5581 ggcattggca taagttagcg cccacggctc atcaaaatag gtttgtaccg actgacgtaa
     5641 ccgctgagca aagacgcggt ttttatccat atcgatcgcg gtacgaataa atttgtgtac
     5701 gtggcggtcg tagccaatcc acaagtcgat ggattgctgg ccccaactga taatacgatc
     5761 gagtttgctc tgcagatcga acaccagacg atcgacgaaa tgcagatcgt catgggtcat
     5821 cgtcgcatcc tgaatgcgca acagattagc ctgcaatttg tcgcctgccg cttccagcgt
     5881 atcctgcaat tcacgcagcg ttccggaagt ttccgaaagc aacaattcac agctggaaat
     5941 cgccgcccgc cagtctttgt tcagcaactg ggcgatatcg tccttcacct gctgctgctg
     6001 ttcgtccatc agacgttgcg tcaggtcgat actgtcgaaa atttctgcta ccgaatattt
     6061 cagtggcgca tagacattac ggtgccagtg aaattcatca ccgccctctt cggcggcatc
     6121 tgctgcgcgt ttgagctcac ccgccacaat cgacaactgc atagaaagac gcagcgtaga
     6181 aaactcgcgc tgacggatgt agtagtcagt aatgccgatg ccgagcggcg tcagacggta
     6241 aattgcgttc ccttccgcct gctcgctggt aaagcggttc agcagacgtt gacgcaccat
     6301 gtcgttgatc gcgttattgg cgcgcacgcc gatggtttcg ctggtttgct caaacgcatc
     6361 actcacatgg cggaatgcat ccaccagctc gccttcactc atctcaccat ccagacgctc
     6421 gccgttcagc gtggcaaccg ccagcagaaa agagagtcgg tctaccggca gcgagatgga
     6481 gaagtcattt tttctggccc aggcaaccag ttcggggact gtctgggaaa attcactcat
     6541 actttatcct tgctctgcgg tttgcgcgcg gtgacatgaa tataacgccc cagggtaata
     6601 tacggttcct gacggcaata acgcgtttct aattcaagta atgcttcata gcagtcgcgc
     6661 tgctggtgct tctcgcgcag ataatcatga aacacgcgaa cgcctgtctt acccataatt
     6721 tgccaaccag cttcttccag ccacagataa acctgcgccg ggtcgcgtgg ataatctggc
     6781 gaaagcgtcc gttttttctt tttcggcatt cccgcctgca cgtaatcaaa attcccggcg
     6841 accatgttat gcatcaacaa accatgcgca ttgtagaaca ttaacgacaa cacgccgcct
     6901 ggacgcaaca ctgaccacag ggtctgcaat acactgcggg gatcggccac ccactcgagc
     6961 accgcatgga acaatatcag atcaacgggc gtttccaaat gcgaagcaac atcctgagcg
     7021 gcgcaatgta taaattgcat gttgtcgctc acaccttttg cttctgccgc ctgttttgcg
     7081 cggtcgatca tctgcgcaga aagatcgcat aaaatgacct gatgcccgcg ctcggccatt
     7141 ttgattgcgg tctgcccttc tccaccgccc gcatccagca cacgcagttt ttgcggaccc
     7201 atttccgcca gcacgcgatc gagatcctgc cacagaatag cctgtcgaag ctgccctttg
     7261 gtggtgccgt aaatgttacg ggaaaacttt tccgcaatat catcaaaatt gcgatcctgc
     7321 attgctttct ccaccggttg gctaagacgt tttctgataa agacgctatt ttgtcacagc
     7381 cacggcgaga atgaacccgt ctggtgtaat atcgctggcg atcccttgct atatggttaa
     7441 aaaaggaacc agaaaggatg ctttttacac tgaaaaaagt gattggcaat atgttgctgc
     7501 cacttccgct gatgttgtta attattggcg ctggcctggc gctgctgtgg tttagccgtt
     7561 ttcaaaaaac cgcgaagatt tttattagca ttgggtggct ggcactcttg ttattgagtt
     7621 tgcaaccggt agccgaccgc ctgctgcgtc ccatcgaaag cacctatccg acatggaata
     7681 attcgcaaaa agtggactat atcgtggtgc tcggaggtgg ttatacctgg aacccgcagt
     7741 gggcaccgag ctctaattta atcaataaca gtctgccacg cctgaatgaa ggtattcgct
     7801 tatggcgcga aaatccggga tcaaaactga tcttcacggg aggcgtagca aaaaccaata
     7861 cggtgagtac agcggaagta ggtgccagag ttgcgcaatc gctgggcgtg ccgcgcgagc
     7921 aaattatcac cctggatttg ccaaaagata ccgaagaaga agctgcggca gtgaaacagg
     7981 cgattggtga cgcccctttc ctgctggtga cctccgcatc acatctgccg cgggcgatga
     8041 tcttttttca gcaggaagag ttaaatccgc taccagcccc ggcgaatcag ttggccatcg
     8101 actcgccgct taatccgtgg gaacgggcaa tcccttctcc ggtctggctg atgcatagcg
     8161 atcgtgttgg ctatgaaacg cttggccgca tctggcagtg gctgaaaggt ccgtcaggcg
     8221 agccacggca agagtgattt tgatgccaga tcgaagttgc gccgactaaa tcgtccggta
     8281 ttaaccagtt gcgcaacttc atcccataac acatacaacc aacgccgcca gatgaacgac
     8341 tccgccactg gcgcgcgttg caggtaactc cagagcaaat cttccgccag agaattatcc
     8401 attagtcgga acagttcgta ttctcgcggt gcccaaagca ttagtcccgg cccgaccatc
     8461 gccagtaact gatcgctgcg cgaatctttc agcatgctgc gtaaacagaa gttaccgtga
     8521 atcaacacac aattgtcgtt aaagccttca aataatgccg ggagacattc gcgagtgcga
     8581 aacaggatcc gcttatcctg catcgtcaga ccggtgttat tgaactgatt gagcgtggtc
     8641 catagcactt caacatgttg ccggtaccat gagggccaga aattttcctg agtattgtcg
     8701 accgcgccga cgcaaccgcg actgtcctga cggtgccagg ccagtaaggc ttcaacgatt
     8761 tggtctttga gctgttccca gcgttctggt gttcgggctg gtgcctccac cgaaacacca
     8821 cgcatccgct ccagtagcag gacatcaggg ccggggtgtt cttcatgcgt catcacacca
     8881 taaatagtcg gcatacggac agtcccactt cttgccagca tggtggtttt ccatgccagt
     8941 tgtcgggctt ttccgggcgt actaaagctt cttgccatta acggcattgg gtttccctgg
     9001 ctgtcataca aagcccacaa cgccgtatcc gctttttcat tgacgcactc aatacgactg
     9061 agtttttcgc ccagtaaatg gcttaattcg gcacgcagct gttccattca gattaccctc
     9121 ataaaagcta ctgctttcat aatgagcgcc aatataaaag atgtcacctg aatgagatca
     9181 attgttgaaa agaaaaattg aggaaaatcg taacggtacg acactcctcc caaaattggc
     9241 tgaagtgtcg tgaagtgaaa ttagcgcatt tcagcgcgaa cgcgctcaag atcttcaggg
     9301 gtatccacac ctgtgccagg aacttcctga gcaacagcaa catggatttt ttcgccgtac
     9361 cacagaacac gaagctgctc taacatttcg atgtgttcta acggacttgc ctgccagttg
     9421 acgtaacgac ggataaagcc tgcacggtag ccataaatac caagatgacg caggaagtta
     9481 tcgccaacgg tttcaaggcc ttctgcaaaa cgatcacgat cccaaggaat ggtggcgcgt
     9541 gagaagtaca gtgcataccc ttcagcgtcg agaaccactt tcaccgcatt cgggttaaac
     9601 gcttcttccg cattgtggat tggcaccgcc agagtcgcca tacccacctg acgctgagcg
     9661 aggttatcag caacctgacg aatgattgtc gcagggatca tcggttcatc accctgcaca
     9721 ttaacgatca ccgtgtcgtc gctgaatgcg catttttcga caacttccgc cagacgttct
     9781 gttcctgact gatgatcggc gcgcgtcata catacttcac cgccagcggc ttcaacggcg
     9841 cgggcaacat cctcatgatc ggttgccacg atgatgcgct cggcacctga ttcacgcgcg
     9901 cgttcaagaa catgaacaat catgggtttg ccgttaatat caaccaatgg tttaccgggc
     9961 agacgcgtgg acgcgtagcg cgcgggaata atgaccacaa aactcatgat ttactctcat
    10021 cagcagtcag cacgcgggct tccgtttcca gcaacactgg aatgccatcg cggagcggga
    10081 aagccaggtt atccagtttg caaataagct cttgtttttc ctggttatac caaagttttc
    10141 cgttgcaaac cgggcaggca atgatttcaa gcaaacgatg atccatagtt cctccagatg
    10201 gatcgggtta tgaatgcata aatcttatca taatcattta tgcgacgggg tctatttccc
    10261 agccagtgcg gcaatcagta aagagacctt gcgggcagcg tcctaatgtc acccgcgttg
    10321 cctgctgcca gctcgcgaaa tcagtaatcg cctgacgtaa ccctttttgc agcattgtcg
    10381 ttggtttaat gccttcctgt aaccacagag agataacttc aaggatgcct gtctggcgat
    10441 gcattttggc atccattcgc ccaactaatt gcccacgatg taataatggc agaacaaaat
    10501 agccatactg gcgtttcggc gctggggtat agcactccag ccggtagcta aaatcaaaaa
    10561 gctgctctgc gcgtttgcga tcccagacaa caggatcaaa aggcgaaagt accgcgctgt
    10621 gcgttgcagt gagctttccg gccagcgcac gctcgagtag cggcagcaaa tcagcatgca
    10681 gccaaagatt gcccaatttt tcaacatgca cagcaatgat ttgctgctgt tcagccctcg
    10741 cttcgcgcca cgccgccagt gccggacgtt tcagccgata gtaatctgcc agccactgtt
    10801 cgcggaatat tcccaagcta cgcgcactgt tatccagcat gatgatttct gcttctgttt
    10861 gcgaaacgag atcgcgctca tcatcccagt caggcatgac acggtgggtt aaatcataaa
    10921 cgcgctggaa gttgcgccgt tcaatcacca tcacctttcc ggcagtaaat aaaccttcca
    10981 gatgccgttt atgcggcttc cattcccacc agccgcttgc acctttacga ggatgctcaa
    11041 aatcggctga acgtaccggc cccttatcat gaatatgctg aattaactgt gcaatttccg
    11101 cctcatgttc ctgcatccag gcgtctttgt atttccagcc cattttttca ggtgccagca
    11161 tgcggtggcg aataagacga aagtcgctac gcggcataaa gcaggcttca tgcgcccagt
    11221 attccattaa ttcgccacgc gccagagact catccagcca ctgggcagga taatttccca
    11281 gacgactgaa aagcaccaga tatggactac gggcaacaat attgatggta tcgatttgca
    11341 gcaaggacat gcgggagatc gttgccggaa tatcctccaa cgacgctcga cggcggggtt
    11401 tgtttaacag gccttgtgcg gcaaggtgaa gattacgcgc atcagcaagg gagaggtgcg
    11461 gcagtgacat tcatgactcc atcaatcgaa cgctgccgcg gcgtaactag ttgccagaag
    11521 ccagcgaggt tagttgcgta agcagtttcg ctggttcatc acctgaaagc tgtgcgtcta
    11581 caggcaaata ccaccaattt tcttctgcaa aggcccggca tttcaccgca tctttttcag
    11641 tcattaccag cgtttgcccg gcgcttacca acgcactgac atccgcatgg ttcaaagact
    11701 gatgatcggc cagcggtaca catttttccg gttgtacgcc acacatcttc agcgtggcaa
    11761 aaaagcgcgg cggatgccca atccccgcca tcgccactac atgttcaagc tgagcaacgt
    11821 cacaacgcgt accggtacgt aaattcaccg cctgacccgg cagcagatgc atggggattt
    11881 caccgctgcg agggacaccg ccgttgacga ttaccgcatc aaccgacttt aagcgccccg
    11941 ctcgctcacg cattggcccc gccggcaacc accagccatt gccaaagcga cgcacaccat
    12001 caataacgac aatttccaca tcacgcgcca gacggtaatg ctgtaaaccg tcgtcggtta
    12061 cgatgatctg cacatcaggg tgttgcgcca gaatggcttt taccgcatca gaacgaacgg
    12121 gagaaaccgc aacaggcgca tcagtgcgtt gataaatcaa cacaggttca tcacccgcct
    12181 gtgctgttgt ggtatctgcc gacaataaca gcggataaga ttcagcctta ccaccatatc
    12241 cccgcgatac gacccccacg cgaataccgc gctgttgcaa ctgttccacc agccagacaa
    12301 cgaccggggt ttttccgttg ccgcctgcgg tgagattacc aaccacgaca accggtacgg
    12361 gggcacgcca ggcgcgcttc agttttagtt tatagcaaag acggatcgcg ccactcacca
    12421 ggccatacaa ccaggagagt ggcagcaata gccgccacaa aggggattca ccagaccaga
    12481 ttttttcgat cattggccaa actgcatttt gtgaagttgc gcgtaaacgc cgcggtgctc
    12541 aagcaaatcg ttatgcgtac cgcgttccac aatgacacca tcctcaacga ccacgatttc
    12601 gtctgccttt tcaatggtag acaagcggtg ggcaatcacc agagaggtac ggtttttctg
    12661 caactcatcc agtgccgcct gaatcgcacg ttcggattcg gtatccaacg ccgaggtagc
    12721 ttcgtccaga atcagaatcg ggctatcacg caacaaggct cgagcgatag caatacgctg
    12781 acgctgaccg ccagagagca gcacgccgtt ttcaccaatc actgtatcga gaccgttatc
    12841 catcttattg atgaagtcta tggcgtaggc catacgcgcc gcttcttcaa tttgctcacg
    12901 gctgtactgt tcagtccgtg cgtaagcaat gttgttagca accgtatcgt taaacagatg
    12961 gacattctgc gacaccagag caacctggtt acgtaacgac gccagggtat actcgcgcag
    13021 atcgtgacca tccatcagga tttcgccttc atcaatatcg taaaaacgcg tgatcaggct
    13081 ggcgatggtt gatttacctg aaccagagcg tccaaccaga gcgaccgttt tccctgccgg
    13141 aattttcagg ttgatgttac gcaatgcggg cacgtcacgt cccggataag taaaggtgac
    13201 attgcggaat tccacgtcgc cagtcgcacg ctcgatcacg cgcttacctt cgtctttctc
    13261 ctgctcactg tccagaatgg taaacagcgt ctgacaagcc gccataccgc gctggaactg
    13321 ggcgttaacg ttggtcagcg atttcagcgg acgcatcagt gcaatcattg aagagaaaac
    13381 aacggtaatc gtaccggcag tcaggttatc cataacactt gggaagctcg ccgcatacag
    13441 aacaaacgcc agcgccaaag aggcgattag ctgaatgatc ggatcagaga tggaagaggc
    13501 tgaaaccatt ttcatcccct gaagacgcat tcggttactg actttatcga agcgtttcgt
    13561 ttccacttcc tgaccaccga aaatcaatac ttctttgtgg cccttcagca tttgttctgc
    13621 gctggtggtc acctgcccca tggtgttctg catgttttta ctgatgttgc gaaaacgctt
    13681 cgatacaacg cgaatcgcaa tcgaaacaat cggtgccagc acaatcaaaa tgatcgacag
    13741 ttgccaactg taatagaaca tcatgatgaa caggccgatg attgacgcac cttcacgcac
    13801 aaccgttatc agtgcgccgg aagaagaaga agcaacctgt tcggaatcgt aggtgatacg
    13861 tgacaacagc gtacccgttg actgtttgtc aaaaaatgaa accggcattc ccatcatgtg
    13921 accaaacagg cggcgacgca tggtcattac cacctttcct gatacccagg agatacagta
    13981 gctggagaca tagctggtga taccacgtaa aatcatcagc ccgatcacca ccagcggcat
    14041 ccacaccagc acggagcgat ctgttttacc aaagccatca tcaagaagtg gcttaaggag
    14101 cgataacatg aaggtatcgc tggctgcgtt gaggattaac gctacgcccg ccacgatcag
    14161 acccgcttta aaaggcgcaa tggttggcca cagtcggcgg aacgtctgcc acgtagagag
    14221 atctttgtcg ttatgcattc aaaaaaccag catttgttga aatagccgca tattctaccc
    14281 gttatccact ggcacgccaa accactgatg ataccaacga ggtaaaattt gctccctgag
    14341 gctgctaatc cgccagcctt gcgctgaaaa attgaccgct atttgcccct gatgaggagt
    14401 atcaagccac ttataaccct gttgttgata gcgatgttta actttgcttg agggcaatcg
    14461 ccatgcgtta tagcgcgatg ccgatgcgag tgccactttt ccattcactc gctgaattaa
    14521 tggcaacgat gatgaggtat tactgccatg gtgaggtacc tgaagcaatg ttgtctgcac
    14581 ttgctgccag taacggctta gcatcttttg ttcagcgggg acttcaatat caccggttag
    14641 aagaatgcta ttcgtcccgt catcaacctt aaccacacag gaatggttat ttcctttatc
    14701 gttgctagct tgtaaaggcc agtgcacgct gaaacgcaat ccttgccatt gccacgcttc
    14761 gccacgcaca cagggctgat gatgttccca gtttaacgga cttctgatcc ataacatcgg
    14821 ccatgtgtgc aatattgagt ccagccctcc ccggtgatcc agatgttcat gactcagaat
    14881 aacgccttcc ggttcaagat tatgccagtg gagccagggg ataatcagtt gttgcccact
    14941 atccccttcg ggccaggcca gtcctgtgtc atagagaatc gctttgccgt ttctggctat
    15001 caccattgcc agcccttgcc cgacatcaag catgtacacc tgccactcgt caggtcgagg
    15061 tttttgccac agcggccagc acatcagcaa gcctacagcc acacacattg ctggcaacgt
    15121 tcgccaggcg tttaatcgcc ataccaccag taagaaccat ggggaaaatg atagccattg
    15181 ccaacgttca gcaatgttga tccacccttc tggcaaactc tttaaccccc agaaaagtaa
    15241 agccaaagac cggtcggcaa gaaaccataa cccttgctcc aggattaacg gcccgcttaa
    15301 atgcacaacc atcgcggcga ggatcaacgg aaccgtgata aatgtcacca agggaattgc
    15361 taatagattt gcaataaacg aggtcagact aatgccatga aatatgacga tttgcacggg
    15421 cataagcaga agtgtgatac ccagttgcag atggatgagg gaaacaactg cacgcaatac
    15481 cggcggcagt tgccactcag gacagggaaa ccactgatac caaaaaatca gtgccgcgac
    15541 cgcagcggca gagagccata aactttgcga gagaatggca acaggatcca tcagcaaaat
    15601 tgccgccaga caacatatcc atacatccca gccactccac tgtcgcccac ttaacttaag
    15661 cattccccac gtagcaagcg ccaccacggt acgcaatgca ggaggttgca ttcccgtcaa
    15721 ccaggcataa aaagcagcac agcagatccc accaattaat ggtatttgcc agtggatcca
    15781 gcgcccaggc agaaaaattt gtccactgcg aatgagtccg gcagccagca acaccgcaaa
    15841 agcgatgtgc aatcccgata tcgccattaa atgcgccgtt ccagtatcgc gcatgatatt
    15901 tttgatttct ttggggacgg ataaccgttc ccccatacct aaaccaagaa taaccgcatt
    15961 ccacggatag ggttgcagcg ttgtttgtag tgacgccaga tactgtgcac gcaggctaca
    16021 attcggttca ataagacttg cctgcagaaa gcggccggtg agcggctgat gctgggcaat
    16081 ggcataacgc tggctatcaa agccgccatc attaagttga ccatgaacgg cacgaacttt
    16141 gagtttcatt gaccacagtt gtccggcaca aaccgcttgc ggaagatatt cgccatacag
    16201 cacaaggcct ggcgcaggga atatacgtcg accttgtaga taagtaattt gaccgtaatg
    16261 agtggtcatg ccgtcagtag cagtgatctc aacaattgca tcctgcgtcg cgccagttaa
    16321 ggtttctcct gcccacaaaa tttgctttgc tgataatatg ccccaaacga aaaacagcaa
    16381 cgtcagcgcg atataacgga cggttttaac aggtataaat gccagtacgc aggcgaagag
    16441 agtcagaaac gcaagggcta atgtcccagg caattggggc aaaatcagca acggaaaaat
    16501 tccgcaaatt atgcatacgt cgactgtcgt tattttcatg cactcctccc tgtttatgga
    16561 aagtgtgcaa ctttgttgag cggttgtctg gtgggaattt tgcgttcccc gaggcacatt
    16621 cagaagaatt taacgagagg gtgaaaactg ctcaaaactc tgcgaggcag cttccagttc
    16681 ctgattagag aaatgaaaaa agcacccgac aggtgctttt ctcgcgttca agtttgagta
    16741 aaaacttaac cgtaaatatt ggcgcgatcg cgcagttctt taccaggttt aaagtgagga
    16801 acgtattttc cttccagttc tactttatcg ccagtcttcg gattacgtcc ggtacgtggt
    16861 gcgcggtagt gcaaagagaa actgccgaaa ccgcggattt caatacgctc gccctgcgca
    16921 agagtcgagg ccatatgctc cagcatctct tttactgcat cttcaaccgt cttggcggga
    16981 atgtgcgatt gctgggtggc aagtctttct atcaattctg acttggtcat gattcctccg
    17041 gttccttaaa ggcaaattaa gcggctgctg ttgctgcatt gattaagggc ggccgtagcc
    17101 gcccttagtg cttgattaca ggacgaaacc tgcaatctgt caagtaaact caacaaactt
    17161 cggaataaaa atcccgaaga gtcagagaat tactcgcctt tagctgcttt gaaagcttca
    17221 gccattgcgt tgttggagaa gtttgcatct tcctgtttgt taacagttgc gattgcatct
    17281 ttctcgtcag cttcgtcttt cgcacgaaca gacaggctga ttgcgcggtt tttacgatca
    17341 acgccggtga atttagcttc aacttcgtcg ccaacgctca gaaccaaggt agcgtcttca
    17401 acgcggtcac gagatgcttc agaagcacgc aggtaacctt caacgccgtc agccagttct
    17461 acggttgcgc ctttagcgtc aactgcagtt actttaccgg ttacgatagc gcctttcttg
    17521 ttcagagcaa cccagttgtt gaacggatct tctgcgagct gtttaacgcc cagggagata
    17581 cgttcacgtt ctgcgtcaac ctgcagaaca actgcagcga tttcgtcgcc ttttttgtat
    17641 tcacgaactg cttcttcgcc tgcaacgttc caggagatgt cagacaggtg aaccaggccg
    17701 tcgatgccgc cgtccaggcc gatgaagata ccgaagtcag tgatagactt gattttacct
    17761 tcaacacggt cgcccttgtt gtgggtttcc gcgaactgct gccacgggtt agctttgcac
    17821 tgtttcagac ccagggagat acgacgacgt tcttcgtcga tatccagaac cataacttcc
    17881 actacatcgc caacgttaac aactttggac gggtggatgt ttttgttggt ccaatccatt
    17941 tcggaaacgt gtaccagacc ttcaacgcct tcttcgattt caacgaagca gccgtagtca
    18001 gtcaggttgg tcacgcgacc agtcagtttg gtaccttccg gataacgttt agcgatagct
    18061 acccacggat cttcgcccag ctgtttcagg cccagggata cacgggtacg ttcgcggtcg
    18121 aacttcagca ctttaacagt gatttcgtcg cccacgttga cgatttcgct cggatgctta
    18181 acgcgtttcc aggccatgtc agtgatgtgc agcaggccgt caacgccgcc cagatcaacg
    18241 aatgcaccgt agtcagtgag gttcttaacg atacctttaa cttccatgcc ttcctgcagg
    18301 ttttccagca gctgatcgcg ctctgcgctg ttttcggatt cgataacggc acgacgagaa
    18361 acaacaacgt tgttgcgctt ctgatccagc ttgattactt taaattcaag ctctttgcct
    18421 tccaggtgca gagtgtcacg caccggacga acgtctacca gagaacctgg caggaacgca
    18481 cgaataccgt tcagctcaac agtgaagccg cccttaactt tgccgttgat aacaccggta
    18541 acagtttcag catcttcgta agctttttcc agcgtgatcc aggcttcgtg acgtttagct
    18601 ttctcacggg acagcagagt ttcaccgaag ccgtcttcta ctgcgtccag agcaacgtca
    18661 acttcgtcac ctacctggat ttccagctcg ccctgggcgt ttttgaactg ctcagccggg
    18721 atggcggact cagatttcag accagcgtca accagtacta cgtctttgtc gatagcaaca
    18781 acaacgccac gaacgataga acccgggcgg gtttcgattt cttttaagga ctcttcaaag
    18841 agttgagcaa aagattcagt catgtttaat cttcaggttt atatttaacg tccacctggc
    18901 tccatgccgg atggggttgt ttaacatacc cgctgtcatt ccattgcaac gggggtactg
    18961 caaattcggt cgcttatgcg agagccaatt tctggcgcgc gtattgtagc gctttttcaa
    19021 tcacttgctc aatgcttaag gtggtggaat ccaacactaa agcatcggat gccggaacca
    19081 gtggcgctac cgctcggtta cgatcgcggt cgtcgcgttc tttgatctcg gccaaaaggc
    19141 gctcaaagtt aacactaaag cccttctcct gcaactgtag catgcggcga tgcgcacgtt
    19201 cttccgagga ggcgtcaagg aaaattttca ctggcgcatc agggaatacc accgttccca
    19261 tgtcgcggcc atcggcaatc aggcctggta attcgcgaaa cgcgcgttgg cgacgcaata
    19321 atgcttcacg aacgcgtggg aatgccgcga cttgtgaagc tgcattcgcc acttcctgag
    19381 tacgaatttc gccgctgaca tcttcccctt cgaggatcac ttccagattg ccattggttg
    19441 acacaaaacg tacatccaga tgggatgcca gcggtaccag cgcatcttct gacgcaacat
    19501 caacatggtg atgtaatgcc gccaacgcca gtacgcgata aattgcaccc gaatccagca
    19561 gatgccattg caatgcttcc gccatagcct tacacaaggt gcctttccct gcaccgcttg
    19621 ggccatcaat ggtaataacc ggggcaattg ccgtcatctt tatctcctta aaacaggcgt
    19681 accgttaaca taaccgcgcg cattatacgc gccgacgcca caaatcgtta acgttgtgtg
    19741 caaaaaccag ttttgtctgt attcagatgg ggatttattg cgcaattata gcgggctggc
    19801 agaagaccag cccgaaagat gacaatgatt tacttaccag aggcgatacg atcacggatg
    19861 tgttgcgcac gctctgttga tggtgggtga gagtcaaaca tggactgggt gcgaccgcca
    19921 tccagactag ccagtttttc aaagctgcca accagcccct gggtgctgat gccacgtttc
    19981 ttcaacagat caaaggagaa atcatctgct tcggactctt tatcacggga gtatttagca
    20041 ttaatagcgc cttctgcgat atcacccaat tgtgagcggg aaagctggga agccacaccg
    20101 ctggtagctg aaatggcatc gcgtgcggca acgatcgcat aagaagcttt catttcagcc
    20161 agcgagtgac ccaacgcgac gtggcccagt tcatggccca gaacaccttc aatttcgttg
    20221 tcattcatca tgtccatcag gccactgtag acacggacac agccgttcgc catcgcccat
    20281 gcgttgacgt cgctggtcat ataaacctta tagttgaccg gcgtgccatt aatattgtta
    20341 cccaatgctt tggcgatttt tgccagacgt ttaccgtact tgctggattt acttgcgact
    20401 tgattgccgc tgtccatttc ggcacagccc tgattggcaa tcgcttttgc atcggcatcg
    20461 cttaatgttg ctgctttata ggcatttaaa ccggagctga tagccatatt ggtatcaata
    20521 ccgtgggtat tttgacaccc tgtcagtaat gctgcagagg tcgcaatcgc cagcagtaat
    20581 ttagtattct tcatgtgaat catccgttga ttaatcatat ataaatatta tgtatcaaca
    20641 acttacattg acaagcgacg ctaccattaa tcaaaatgac tcacaaggtc cgaaaaaaaa
    20701 cgccgacaat g
//
LOCUS       DADMKF010000067        20547 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.068, whole genome shotgun sequence.
ACCESSION   DADMKF010000067 DADMKF010000000
VERSION     DADMKF010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20547
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.068"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            753..1181
                     /gene="uspC"
                     /locus_tag="JNC24_002230"
     CDS             753..1181
                     /gene="uspC"
                     /locus_tag="JNC24_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HAY9158970.1"
                     /translation="MSYSNILVAVAVTPESQQLLAKAVSIARPVKGHISLITLASDPE
                     MYNQLAAPMLEDLRSVMQEETQSFLDKFIQDAGYPVDKTFIAYGELSEHILEVCHKHH
                     FDLVICGNHNHSFFSRASCSAKRVIASSEVDVLLVPLTGD"
     gene            complement(1188..2612)
                     /gene="otsA"
                     /locus_tag="JNC24_002231"
     CDS             complement(1188..2612)
                     /gene="otsA"
                     /locus_tag="JNC24_002231"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase"
                     /protein_id="HAY9158971.1"
                     /translation="MSRLVVVSNRIAPPDEHAASAGGLAVGILGALKAAGGLWFGWSG
                     ETGNEDQPLKKVKKGNITWASFNLSEQDLDEYYNQFSNAVLWPAFHYRLDLVQFQRPA
                     WDGYLRVNALLADKLLPLLQDDDIIWIHDYHLLPFAHELRKRGVNNRIGFFLHIPFPT
                     PEIFNALPTYDTLLEQLCDYDLLGFQTENDRLAFLDCLSNLTRVTTRSAKSHTAWGKA
                     FRTEVYPIGIEPKEIAKQAAGPLPPKLAQLKAELKNVQNIFSVERLDYSKGLPERFLA
                     YEALLEKYPQHHGKIRYTQIAPTSRGDVQAYQDIRHQLENEAGRINGKYGQLGWTPLY
                     YLNQHFDRKLLMKIFRYSDVGLVTPLRDGMNLVAKEYVAAQDPANPGVLVLSQFAGAA
                     NELTSALIVNPYDRDEVAAALDRALTMSLAERISRHAEMLDVIVKNDINHWQECFISD
                     LKQIVPRSAESQQRDKVATFPKLA"
     gene            complement(2587..3387)
                     /gene="otsB"
                     /locus_tag="JNC24_002232"
     CDS             complement(2587..3387)
                     /gene="otsB"
                     /locus_tag="JNC24_002232"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HAY9158972.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTVIAQYPGAELEAKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITTALQQKRENNRSDDYESFSRSI"
     gene            complement(3554..4543)
                     /locus_tag="JNC24_002233"
     CDS             complement(3554..4543)
                     /locus_tag="JNC24_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose ABC transporter permease AraH"
                     /protein_id="HAY9158973.1"
                     /translation="MMSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVP
                     NFATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTES
                     LWIGVAAGLLLGILCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIED
                     ESFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPV
                     VRTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGK
                     ISYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRIV"
     gene            complement(4555..6069)
                     /locus_tag="JNC24_002234"
     CDS             complement(4555..6069)
                     /locus_tag="JNC24_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose ABC transporter ATP-binding protein
                     AraG"
                     /protein_id="HAY9158974.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLSIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIDNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKAPGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGLFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEDRKAEGIIPVHSVRDNINISARRKHVLGGCVINNGWEENNADQHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRSIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            complement(6139..7128)
                     /locus_tag="JNC24_002235"
     CDS             complement(6139..7128)
                     /locus_tag="JNC24_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001397210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY9158975.1"
                     /translation="MHKFTKALAAIGLAVVMSQSAMAENLKLGFLVKQPEEPWFQTEW
                     KFADKAGKDLGFEVIKIAVPDGEKTLNAIDSLAASGAKGFVICTPDPKLGSAIVAKAR
                     GYDMKVIAVDDQFVNAKGKPMDTVPLVMMAATKIGERQGQELYKEMQKRGWDVKESAV
                     MAITANELDTARRRTTGSMDALKAAGFPEKQIYQVPTKSNDIPGAFDAANSMLVQHPE
                     VKHWLIVGMNDSTVLGGVRATEGQGFKAADIIGIGINGVDALSELSKAQATGFYGSLL
                     PSPDVHGYKSSEMLYNWVAKDVEPPKFTEVTDVVLITRDNFKEELEKKGLGGK"
     gene            7925..8428
                     /locus_tag="JNC24_002236"
     CDS             7925..8428
                     /locus_tag="JNC24_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HAY9158976.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(8507..8758)
                     /locus_tag="JNC24_002237"
     CDS             complement(8507..8758)
                     /locus_tag="JNC24_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 domain-containing protein"
                     /protein_id="HAY9158977.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTNHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(8873..8959)
                     /gene="azuC"
                     /locus_tag="JNC24_002238"
     CDS             complement(8873..8959)
                     /gene="azuC"
                     /locus_tag="JNC24_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HAY9158978.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            9222..9545
                     /locus_tag="JNC24_002239"
     CDS             9222..9545
                     /locus_tag="JNC24_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9158979.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAVPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            9716..10213
                     /gene="ftnA"
                     /locus_tag="JNC24_002240"
     CDS             9716..10213
                     /gene="ftnA"
                     /locus_tag="JNC24_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HAY9158980.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(10251..10490)
                     /locus_tag="JNC24_002241"
     CDS             complement(10251..10490)
                     /locus_tag="JNC24_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family protein"
                     /protein_id="HAY9158981.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLAAKGKFIPSKDGFSTDQSKICRH"
     gene            10681..11892
                     /gene="tyrP"
                     /locus_tag="JNC24_002242"
     CDS             10681..11892
                     /gene="tyrP"
                     /locus_tag="JNC24_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HAY9158982.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGV
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNVRKLRWVF
                     ITGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(11954..12619)
                     /gene="yecA"
                     /locus_tag="JNC24_002243"
     CDS             complement(11954..12619)
                     /gene="yecA"
                     /locus_tag="JNC24_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HAY9158983.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTNHAILDVAELDGLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(12815..12901)
                     /locus_tag="JNC24_002244"
     tRNA            complement(12815..12901)
                     /locus_tag="JNC24_002244"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12865..12867),aa:Leu,seq:taa)
     gene            complement(12914..12987)
                     /locus_tag="JNC24_002245"
     tRNA            complement(12914..12987)
                     /locus_tag="JNC24_002245"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12953..12955),aa:Cys,seq:gca)
     gene            complement(13042..13117)
                     /locus_tag="JNC24_002246"
     tRNA            complement(13042..13117)
                     /locus_tag="JNC24_002246"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13082..13084),aa:Gly,seq:gcc)
     gene            complement(13269..13817)
                     /gene="pgsA"
                     /locus_tag="JNC24_002247"
     CDS             complement(13269..13817)
                     /gene="pgsA"
                     /locus_tag="JNC24_002247"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HAY9158984.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(13874..15706)
                     /gene="uvrC"
                     /locus_tag="JNC24_002248"
     CDS             complement(13874..15706)
                     /gene="uvrC"
                     /locus_tag="JNC24_002248"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HAY9158985.1"
                     /translation="MSDQFDAKAFLKTVTCQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAARIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(15703..16359)
                     /gene="uvrY"
                     /locus_tag="JNC24_002249"
     CDS             complement(15703..16359)
                     /gene="uvrY"
                     /locus_tag="JNC24_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HAY9158986.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     TNAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            16655..16831
                     /locus_tag="JNC24_002250"
     CDS             16655..16831
                     /locus_tag="JNC24_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9158987.1"
                     /translation="MIKIFIGHYINAFNSIVDVTLKKQPLLFLAKLMVYSAALTFFTA
                     NFHCNMTRKINEYA"
     gene            16818..17042
                     /locus_tag="JNC24_002251"
     CDS             16818..17042
                     /locus_tag="JNC24_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein"
                     /protein_id="HAY9158988.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(17109..17831)
                     /gene="sdiA"
                     /locus_tag="JNC24_002252"
     CDS             complement(17109..17831)
                     /gene="sdiA"
                     /locus_tag="JNC24_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
                     /protein_id="HAY9158989.1"
                     /translation="MQDTDFFSWRRTMLLRFQRMEAAEEVYHEIELQAQQLEYDYYSL
                     CVRHPVPFTRPKVAFYTNYPESWVSYYQAKNFLAIDPVLNPENFSQGHLMWNDDLFSE
                     AQPLWEAAHAHGLRRGVTQYLMLPNRALGFLSFSRCSTREIPILSDELQLKMQLLVRE
                     SLMALMRLNDEIVMTPEMNFSKREKEILKWTAEGKTSAEIAMILSISENTVNFHQKNM
                     QKKINAPNKTQVACYAAATGLI"
     gene            complement(18061..18813)
                     /gene="yecC"
                     /locus_tag="JNC24_002253"
     CDS             complement(18061..18813)
                     /gene="yecC"
                     /locus_tag="JNC24_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     YecC"
                     /protein_id="HAY9158990.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPQQPRTRQFLEKFLLQ"
     gene            complement(18810..19478)
                     /gene="tcyL"
                     /locus_tag="JNC24_002254"
     CDS             complement(18810..19478)
                     /gene="tcyL"
                     /locus_tag="JNC24_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HAY9158991.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(19493..20479)
                     /gene="dcyD"
                     /locus_tag="JNC24_002255"
     CDS             complement(19493..20479)
                     /gene="dcyD"
                     /locus_tag="JNC24_002255"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HAY9158992.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDDVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGVIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCEALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEIILWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIGCISQKRFKDEGPILFIHTGGAPALFAYHPHV"
BASE COUNT         5257 a         5227 c         4726 g         5337 t
ORIGIN      
        1 tttttatgcg gtttcaccgc accccgtgat gtcgccggga agccccggta aaaaataatt
       61 agcattagaa tagttgcgat aagctgcaat aagcagaacc acctttttgg tttaatatgt
      121 ccttacaaat agaaatgggt ctttacactt atctaagatt tttcctaaat cgacgcaact
      181 gtactcgtca ctacacgcac atacaacgga ggggggctgc gattttcaat aatgtgtgat
      241 gcagatcaca caaaacactc aattacttaa cataaatgta taagtcatac ttttgttttg
      301 ggtgtatttc aatatgttaa aaagtttttc gctacgctag caagcaaaaa tgaaacagga
      361 ataatcgaaa tgggatgttg cgcacagtta aaataaatca ccgtaaataa tcatctgcta
      421 taaataatca ctttcatgca ataccagata agctattttt aaacagacac ttaccacaca
      481 acaaactaat taaatacaaa gaaaggcaac aatcaactta atgttaatga aatgttttac
      541 atacattaac cacttgttta atgcggagaa tcagtacact attttcttat aattacattt
      601 gaaattatat gatcaccggg agtgattgat taattgtctt tactaataat cagactaata
      661 tttacctgtt tgaccgagtt gggattgcgt cgtttctcca ttaggagtaa agctttaatg
      721 tcacctgaag ttcacagaat aaggaacagg ttatgagcta tagcaatatt cttgtcgctg
      781 ttgcggttac accggaaagt cagcaactgc tggcaaaagc ggtatctatc gccaggccag
      841 taaagggaca catcagttta attactctcg cttccgaccc ggaaatgtac aatcaattag
      901 ccgcgccgat gcttgaagat ttacgcagcg tgatgcaaga agaaacgcaa agctttcttg
      961 ataagttcat tcaggatgcg gggtatcccg ttgacaagac gtttattgcc tacggtgagt
     1021 taagcgaaca tattctggaa gtatgtcaca aacatcattt cgatttggtg atttgcggta
     1081 atcacaatca cagtttcttt tcgcgagcat cctgctcggc gaaaagagtg attgcctcaa
     1141 gtgaggtcga tgtgctgtta gttccactta cgggagatta accgctccta cgcaagcttt
     1201 ggaaaggtag caactttatc gcgctgctgg ctttccgcgc ttcgcggaac tatctgcttt
     1261 aggtcgctaa tgaagcactc ctgccagtgg ttaatatcgt ttttcacgat aacgtccagc
     1321 atttctgcat gacgggaaat acgttccgcc agcgacatag tcaatgcacg atccagcgca
     1381 gctgcaactt cgtcacgatc gtaggggtta acaattaacg ccgacgttaa ctcgtttgcc
     1441 gctcccgcaa attgcgaaag aacaagaacg cccgggttgg ctgggtcctg agcagcaaca
     1501 tactcttttg ctaccaggtt catcccgtca cgcagtggcg tcactaagcc cacgtcagag
     1561 tagcggaata ttttcatcag taatttacgg tcaaaatgct gattcaaata ataaagcggc
     1621 gtccagccta attgcccgta tttaccatta attcgtccag cttcattttc gagctgatga
     1681 cgaatatcct gataggcttg cacatcacca cgcgacgttg gtgcaatctg ggtataacga
     1741 attttaccat gatgctgcgg atatttttcc agcaacgctt cataggcgag aaaacgctct
     1801 ggcaaacctt tggaataatc cagccgttcg acagaaaaga tattttgtac gtttttcagt
     1861 tccgctttaa gttgcgccag ttttggcggc agtggcccgg cagcctgttt ggctatttct
     1921 ttcggttcaa tgccgatcgg gtagacttct gttcgaaatg ctttgcccca ggctgtatgg
     1981 ctttttgcgc tacgtgtcgt gacgcgggtc aggttagaaa gacaatccag gaacgccaga
     2041 cgatcgtttt ctgtctggaa acccagcaaa tcataatcac aaagctgttc aagcaaggtg
     2101 tcatatgtcg gcagcgcgtt gaagatttcc ggtgtcggga aaggaatatg cagaaagaaa
     2161 ccaatgcgat tattcactcc ccgtttgcgt aattcatgcg caaatggcaa caggtgataa
     2221 tcgtggatcc agataatgtc atcgtcttgc aacagcggca gtaatttatc tgccagcaac
     2281 gcatttacgc gtagatagcc gtcccaggca ggacgctgaa attgcaccag atcgagccga
     2341 taatgaaaag cgggccagag aacggcattg gagaattggt tgtagtattc gtcaaggtcc
     2401 tgttcgctga ggttaaaaga ggcccacgta atgttacctt ttttcacctt ttttagcggc
     2461 tgatcctcat tccctgtttc accactccag ccaaaccaca gtccgcctgc ggctttcagt
     2521 gcccccagta tgccaacggc aaggccaccg gcactggcgg cgtgctcgtc tggtggtgca
     2581 atccggttag atactacgac taaacgactc atagtcatca ctcctgttat tttctctttt
     2641 ttgttgtaat gcggtggtta tcatttcaag ccagctccag acatccggca cacccgccag
     2701 tcgccatgat gcctgagttg cacctgtgcc aatttttact gacattccgc ccagtcggtt
     2761 aacgactgcg aagccagatt catcggttaa atcatcgccc agaaatacgg gcgttcgccc
     2821 gataaaggga gcttcctgca taaaagctgc aattgcctca cctttactgg tacctctcgg
     2881 tttgatctcg acaacacact ttccctgctg taacgccatt tgtggccaga tctgagtaat
     2941 acgttgcgct aatgtcatta atgcgtcttc atgctgcgga gcctgacgat aatgcagcgc
     3001 aaaagccatc cctttcgcct ccagctccgc gccgggatac tgagcgatga ctgtatgcag
     3061 ttgcacgcta atatcacgcg caatcgcatc cggcagatga acgatatgtg ttttaccatt
     3121 gatgtcacgg cgctccgccc catgcacgcc cgctaacggg aagcgataag gttttgccag
     3181 tgcgtcaagc tccaccattg agcgccctga tatcaatgcc aatgcaccat cacttgcggt
     3241 tgccagtagc tgtagtcctt gcagaatatt gtcaggcacg acgacctgat cgggatgcgg
     3301 tttgatttcc gccagcgttc catcaagatc aaaaaaccag gcatatttcg cggatagttc
     3361 aggggtttcg gttaacggtt ctgtcacccg gctctcctcc ttctcttttc ttattgtttt
     3421 gcgcagaacg cgctttacag acatgtaagt atagacagtg tgacgggggt cgccatttgc
     3481 aatgaagaac agccaaccgt tgtggcggct ggctgtggtg ggaaaaaacg ttaaattgtt
     3541 gtggaaaaaa gcatcagaca atgcgtttcg ctttttgctt gtaacggtcg aagatcaccg
     3601 ctgccagcag gattaagccg cgaaccacgt actgcgcgaa aggagaaata ttaagcaggt
     3661 tcatggcgtt ttccacggtg cctaaaatta agatacccgc caccacatat gagatttttc
     3721 cgatgccacc tttcagagaa acgccaccta aaacgcaggc ggagataaca ataagctcat
     3781 aaccaatcga cgtcattggc tgcccactag tcatacgtga agccagaata attccggcta
     3841 tcgcagatac caggccagag agaacaaaga taataatttt ggtgcgaaca accggtacac
     3901 ccgccagacg cgcagcctct tcgttcccgc caatcgccag ggtgttacga ccaaaggtgg
     3961 ttttattcag caacaaacca aagataatca gacacgcgac ggtgagccag attggcgcag
     4021 gcagaccgaa ccagttagcg taaccaaggg caaagaagct ttcatcttcg ataccgaccg
     4081 ctttaccgtc tgaaatgatg tacgccagac ctcgaacaat ctgcatcgtt gccagtgttg
     4141 tgatcagagc atttattttc agtttggcga taacaaagcc attgaccagg ccacagagaa
     4201 tgccaagcag caaccccgct gccacgccaa tccacaggct ttcagtcagg ttgataacca
     4261 ccgccgtggt gacacccgca caggcaatta cggaggcgac agaaaggtca aagtcaccgg
     4321 aagcaaggca gaacaacatg ccacacgcca ccatccccga catggaaatt gccaggccca
     4381 accctttcat attaatgaag gtggcaaaat ttgggacaaa aatggcacag gcgataaaga
     4441 gcaccgcaaa caccaccagc atgccgtact gatcccagat acgcccgaag ctgaatgacg
     4501 acttaggtgc gccagaaccc gatgtagaaa cagaagacat catactctcc ttactcaggc
     4561 aactgcctgg ctgactttag gcatcgcaag gctcagtgcc tgacgctcat ctgcctgctc
     4621 gtgtaacaat tcaccggcga tttcaccttc ccgcatcacc acaatccggt cggcaacgcc
     4681 gaggacttca ggtaagtcgc tggaggcaaa cagcaccgcc acaccctgcg ccgccagcgc
     4741 ataaattacg ttatatattt cgtgcttagc accaacatca atgctgcgcg taggttcatc
     4801 cagcaaaatg accttcatct cttccgataa ccagcggccc agaatggctt tttgctgatt
     4861 tccgcctgag agattcatga tcagttgctc agcgcccggc gttttgatgt tgagcgaacg
     4921 aatgtgctga tcggcattgt tttcttccca accgttgttg attacacaac cgccgagcac
     4981 atgtttgcgt ctggcactga tgttgatatt gtcgcgaacg gagtgcacgg gaataatgcc
     5041 ttccgctttg cgatcttccg ggcagagcat catgcctgcg gcaatggcgt ggctcggttt
     5101 acgaatatcg atcggctgtt ggtcgatata aacctgaccg gcggtgattt gcgtcccgcc
     5161 aaacaagcct ttcattaatt cgctacgccc cgcccctacc agaccaaaca gcccaacaat
     5221 ttcaccactg cgaaccgcca gacttattgg cgtacgcacg cctggtgctt tcacagcatc
     5281 aagacgcagg cgctcctcgc cataactacg aggttgccag ccgtagatat cgccaatgtc
     5341 gcgcccgacc atcgcctgca ccagcgcgtc gtggtcaacc tgctgcatat cggtaaaggt
     5401 tttgacataa cgtccatctt taaagacagt gatggcatcg ctgagggcaa atatttcttc
     5461 catacggtga gaaacgtata agatcacccg cccctctttt cgcagttcac gaataacgcg
     5521 gaaaagattg tcgatttcgc gggcagaaag ggagctggtt ggctcatcaa aggcgataat
     5581 tttagcgtta cgcgccagcg ccttggcgat ttcaaccatc tgccactgac caatggagag
     5641 atatttcagc ggcgtgtccg ggtcaatatc cataccaaga tgtttaagtt gtaaacccgc
     5701 ctcataattc agcaatgagc gattcacaat gccgccttta tgcggcagct ggccgagata
     5761 gatgttttcc gcgacggtca tttccggcac gagatgcagt tcctggtaaa taatcgccac
     5821 acccgcgtta agtgctgcgg tcgtgtcgga aaaggacatt tcctgcccat taatcactac
     5881 agaacccgtg gttggcgcat agttgccgct gaggattttt aagagagttg attttcctgc
     5941 gccattttca cccatcaacg catgaacctg accggcatag cagtcaaaac taatatccgt
     6001 cagcgcctta acgccgggaa acgttttacc gatgccgcga aatgagagat acggggtaga
     6061 ctgttgcata acgtctccgt gaatcactgg tcgttcacac cccctctgca tcatgcagag
     6121 gggaattttt ccggcaaatt acttaccgcc taaacctttt ttctccagtt cttctttaaa
     6181 gttgtcacgc gtgatcagca ccacgtcggt aacttcggta aattttggcg gttcaacgtc
     6241 ttttgctacc cagttgtaaa gcatttcgct ggatttatag ccatgtacgt ccgggcttgg
     6301 cagcagggaa ccgtagaagc cggttgcctg tgctttagac agttcgctca acgcatccac
     6361 accgttaatg ccaatgccga tgatatcggc agctttaaag ccctgacctt ccgtcgcgcg
     6421 tacgccgccc agcacggtgc tgtcgttcat accgacgatc agccaatgtt taacttccgg
     6481 atgttgaacc agcattgagt tggcagcgtc aaatgccccc gggatgtcgt tagatttggt
     6541 aggtacctga taaatttgtt tttccgggaa tccggccgct ttcagcgcat ccatagaacc
     6601 cgttgtacgg cgacgtgccg tatccagttc gttggcggta atcgccatca ccgcgctttc
     6661 tttgacatcc cagccacgtt tctgcatctc tttatacagt tcctgaccct gacgttcgcc
     6721 aattttagtc gccgccatca tcaccagcgg aacggtatcc attggcttac ctttggcgtt
     6781 aacaaactgg tcatccacgg caatgacttt catatcgtag ccacgcgctt tcgcgacgat
     6841 ggcagagccg agtttggggt ccggagtaca aataacgaaa ccttttgcgc cactggcagc
     6901 caggctgtcg atcgcgttca atgttttttc gccatccggc acggcaatct taataacctc
     6961 aaaccctaaa tccttcccgg ctttatcggc aaacttccat tcggtctgga accacggctc
     7021 ttccggttgc ttcaccagaa aaccgagctt caggttctcc gccatagcgg attgtgacat
     7081 aacgactgcc agaccaatgg ctgccagggc tttagtaaat ttgtgcatgg ttctctccag
     7141 ctttagtgtc gttttgtgta gggcaaaaac gaataacttt cgttaaatta atcggaaaac
     7201 aaagcattac cttttatcta aaagataagt gaccgtgttg acatagtttt agcgagaaat
     7261 taattctcca taggagagca atatcacatc gcagaattac agtgagaacg tgcataaatt
     7321 tagcgggaaa agacataagg gaaagccaat ttgtcagaca aattgtcgaa tgcacagcag
     7381 attaatccat aagattagcc tggaaatcct tgttgtcttt ggtacccatg cgggatgtct
     7441 tctttttaac cagtcaatag gccgcattac ctggcgttga gtttttgaaa tggtgtaata
     7501 accgcaactc aaagatgtgg aaaatgcacg tcattcattt cgccattaat tatcactgtg
     7561 ctcattaatt aacagaacac gtataatgag agccatctcg caaaaatgaa aaaacgtttt
     7621 ataaaatcat cacttcatca tgaattcaaa ttcattgatt aatatcaaca agatacaaaa
     7681 agcactatca ttaaaattca ttgcagttac attgatttca tcaatgaaat gtaaaaatat
     7741 ataaacttga tgatttaagc attttcttat acccgttcag acgttattct tatttcagat
     7801 catcgtcaga attgactcca cgatcacatt tcggaccggc agaaaggaat tattctgcaa
     7861 acagtaatta tggtgttttg atttatcttg cacctctcca cttctggata taaggatatt
     7921 aggtatggca accgctggaa tgcttctcaa actcaactct caaatgaacc gcgagtttta
     7981 cgcatccaat ctctaccttc acctgagtaa ctggtgttct gaacagagtc tgaacggcac
     8041 cgccactttc cttcgcgccc aggcacagag taatgtgacc caaatgatgc gcatgtttaa
     8101 ctttatgaag agtgtcggcg ctacccccat cgttaaagcc attgatgttc ccggtgaaaa
     8161 actgaactct ctggaagaac tgttccaaaa aacgatggaa gaatacgagc aacgttctag
     8221 tacgttggca cagttagccg atgaagcgaa agaactgaat gatgattcaa ccgtcaattt
     8281 cctgcgcgat ctggaaaaag aacagcagca tgatggtctg ttgctgcaaa ccattcttga
     8341 tgaagtgcgc agtgcgaaac ttgcgggtat gtgccctgtg cagaccgacc aacatgttct
     8401 gaatgtcgtg tcacaccagc tgcattgatc atcatcggcg ctaatgcatt gcgccgatga
     8461 aggttttgag aaaccgctgc ctcatctgtt tgaagcagcg gtttttttaa tgggattcac
     8521 cctgtggggt aaacttgagt tcaataagcg cgatggcttt ttggattgcc cgcatggtga
     8581 ccgggtctgc ggcggcggga tggttagtaa agtcgatatt cttcagctga ctggacattt
     8641 tttcacgaac ttcaacgggc gcgattacat cgagaacatc cagaatttgt ttgataacca
     8701 actggcaagc aaccacatca gaaaccagtt cctgatcggc attcagcggc tgggacatcg
     8761 taaactcctg atagcatttt gaaagccgtt atagtagcgg cttcacatct tcagcgatag
     8821 tcacatccac cgtcatcagg acacaaaaaa acctgccgga gcaggttttt tgttatcgga
     8881 acatattgcc tggcggtacg tctttgaacg tcttgcaata gttattgaac atacttttca
     8941 ggattttgcg cagtttcatc gcggcactcc gaccatttgt tatacaggtg ttattgtctt
     9001 tgcgcttata atatgacaac catcacaaaa atcaatcttt atgtgataca aatcacataa
     9061 ataccccttt aatgttataa aaatgataat caaaaaacag cccccctatt tctgacacct
     9121 acagatggca agaaatagcg cctgccaggc gtcttttccg gccattgtcg cagcgctgta
     9181 acgcgtaaaa tagtgctttc tcttactctt ctggctggac catgagactt ctgattctga
     9241 ctctttcatt aataacgctg gcggggtgta cggttactcg ccaagcccat gtgagcgaag
     9301 ttgacgcggc aacaggtatt gtacggttgg tttatgacca ggcttttttg cagcatgccc
     9361 ataccgatcg ttatgtcagt cgcgggatcg ccgatcgcgc atgccagcag gaaggctata
     9421 cccacgcggt cccctttggt cagccagtag gcaactgtag cctttttgcg ggttccttat
     9481 gtttgaatac ggaattcact ttgtcatatc agtgccatca ttccgccttc cctgtctttc
     9541 tctaaatatt ttaatcagcg aggggatctt cgctgattaa agaaatagcg gtaatgcgtt
     9601 ttattcccat tcgtattttt aataattgaa gtttatattt taccttttgc aaataataaa
     9661 ataacaaatt atagtgacgc cacagcaaca aatataacct ttgtggagca ctatcatgct
     9721 gaaaccagaa atgattgaaa aacttaatga gcagatgaac ctggaactgt actcttcact
     9781 gctttatcag caaatgagcg cctggtgcag ctatcatacc ttcgaaggtg ctgccgcgtt
     9841 cctgcgccgt cacgcccagg aagagatgac gcatatgcag cgtctgtttg attacctgac
     9901 tgataccggc aatttaccgc gtattaatac cgttgaatct ccgtttgctg aatattcctc
     9961 acttgatgaa ttattccagg aaacctataa acacgaacaa ttaatcaccc agaaaattaa
    10021 cgaactggct catgctgcaa tgaccaatca ggactaccca acatttaatt tcctgcagtg
    10081 gtatgtttct gagcagcatg aagaagagaa actgttcaaa tcgattattg ataaattaag
    10141 cctggcaggc aaaagcggcg aaggtctgta ttttatcgac aaagaactct ctaccctcga
    10201 cacacaaaac taatgctctc cggcggcagg tttactgccg ccgtttcaaa tcagtgacgg
    10261 caaatcttac tctgatcggt cgaaaaaccg tctttcgaag gtataaattt gccttttgct
    10321 gccagaaacg ctaccagctc tcccgctgtc atcccttctg ccgagcaggt gtgaaaacgt
    10381 gcctgttcac caaaacgcgc tttaatcgca gcttccagac tggcatgcgt atattgctcg
    10441 cctgattcaa tcatcatatt taacacttca tgaccgtgaa tagagtccat cgttcctcct
    10501 caaaaaaagc ctagcgtagc gattgccgct tatgaagact ttgcgccagc gcaggactga
    10561 atgcttttta ttgtacattt atatttacac tatatgtaac gtcggtttga cgaagcagcc
    10621 gttatgcctt aacctgcgcc gcagatatca ctcataaaga tcgtcaggac agaagaaagc
    10681 gtgaaaaaca gaaccctggg aagtgttttt atcgtggcgg gaaccacaat tggcgcaggc
    10741 atgctggcaa tgccgctggc tgcggccggt gttggtttta gcgttacgtt aatcttgttg
    10801 attgggcttt gggcgttgat gtgctacacg gcgctattac tgctggaggt gtaccagcat
    10861 gttccggcag ataccggtct gggcacgctg gcaaaacgct atctgggacg ctacggtcaa
    10921 tggctgacgg gcttcagtat gatgttctta atgtatgctc tgactgcggc atacatcagc
    10981 ggtgtcggtg aattgttggc ctccagcatc agcgactgga caggtatttc aatgtcggca
    11041 accgctggcg tgctgttgtt cacttttgtt gccggtggcg tggtttgtgt tggaacatcg
    11101 ctggtcgatt tatttaaccg ttttctgttc agcgccaaaa ttatttttct ggtggtgatg
    11161 ctggtattgc tgctaccgca tattcacaaa gtgaatcttt taaccctgcc gttgcaacag
    11221 gggctggctc tgtcagcaat cccggtgatt tttacctcat ttggttttca cggtagcgtg
    11281 ccgagtattg tcagctatat ggatggcaac gttcgtaagc tacgctgggt gtttataacc
    11341 ggtagtgcga tccccctggt ggcatatatt ttctggcagg tggcaacgct tggcagcatt
    11401 gattcaacaa cctttatggg attgctggct aatcatgctg gattaaacgg gctgttacag
    11461 gcgttacgcg aaatggtggc ctctccgcat gttgagctgg cagtgcattt atttgctgat
    11521 ttagccctcg ccacgtcatt tctcggcgtt gcgttaggct tatttgatta tctggctgat
    11581 ttatttcagc gttcaaatac cgttggtgga cggttgcaaa ctggtgcaat tacctttctg
    11641 ccgccgttgg cgtttgcact gttttatcca cgaggatttg tgatggcgct gggttacgcc
    11701 ggtgtggcgc tggcggtact ggcattgatt atcccttcgc tgttgacctg gcaaagcaga
    11761 aagcacaatc ctcaggcggg ttaccgggtc aaaggtggtc gtccggcgct ggtggtggtg
    11821 tttctctgtg gtattgctgt gattggcgtg caatttttga ttgcggcagg gttgttacca
    11881 gaagtggggt gatcagatag cctcaaattc cttattgggt gccagaatta acgctgacac
    11941 ccaatttggc ctcttaatgc aggcagcact gcttaaattt cttaccacta ccgcacgggc
    12001 aaggatcgtt acgccccggt ttctcttctg ctttgatcgg ttgctgaaca gctttttcct
    12061 gcggatgcgc catccagtac gcatgtagat caagcgccgc cagtcgaatg gcatctacgc
    12121 tctcttcaaa cgcttctggc gacatctttt ctacccgctc gaagttttcc tcagtaccgt
    12181 gcagcgcaat cgcctccagc gctggtttta acgaatcggg caacgttgac cagtcagaaa
    12241 gtgccacgcc ccgcatatag ccaaagcacc actcctcaac aatcgtcagc tcgctgccat
    12301 caacttctcg caagccgaat aacggctcaa actgctccgg gaattcgttc agacgctctg
    12361 cggtatcggc catatgttga aaagccaggt tcataaagcg cgtcatctct ttctctgacg
    12421 cccagcgcgg cacatagtca gccccacccc acacggcaac cagccactgt tccggttcaa
    12481 tctcttgcgg agaactcaac accgccgtca ataaaccgtc cagctccgcc acatcaagga
    12541 tggcgtggtt agtgttgtat ttggtcagaa tatcgtccag ccattccaac tcactttcgt
    12601 ttaacggtcc cgttttcata cgcttttcct tgtggatctc aactcgccag cacctatctt
    12661 acatgccggt ccgtatcaga gatacttttt gagtggcttt gccggtgatt aaaaattaag
    12721 gagggtgtaa cgacaagttg caggcacaaa aaaaccaccc gaaggtggtt tcacgacact
    12781 gcttattgct ttgattttat tcttatcttt cccatggtac ccggagcggg acttgaaccc
    12841 gcacagcgcg aacgccgagg gattttaaat cccttgtgtc taccgattcc accatccggg
    12901 ctcgggaaga aagtggaggc gcgttccgga gtcgaaccgg actagacgga tttgcaatcc
    12961 gctacataac cgctttgtta acgcgccaaa ttcttcaggc ctttcagcca gacatccgct
    13021 tgacgccgat gtcttttaaa ctggagcggg aaacgagact cgaactcgcg accccgacct
    13081 tggcaaggtc gtgctctacc aactgagcta ttcccgcatt catcaagcaa tcagttaatc
    13141 acttgatttt attatcgtct ggcaatcagt gccgccgttc gatgcgttgc attctactta
    13201 cctggcgcga tgagtcaacg atatttttca ccacttttga tcgtttgctg aaaattacgc
    13261 cgaaacgatc actgatcaag caaatctgca cgcgcagcgc tcaaatattg caacattgac
    13321 cacagagtca gtaccgcagc cacaaagaaa agtgcaatac cggcgtactc aacccaaatg
    13381 ttcggacgcc acagcagcca tgccaacgcc accatctggg cagtggtttt cactttccca
    13441 atccaggaga cagccacgct actgcgttta cccaactccg ccatccattc gcgtagcgca
    13501 gaaataataa tttcacgggc gatcatcgtt gctgccggta aggtcaccca ccagctgtgg
    13561 taatgctcgg ttaccagcac catggcgata gccacgagaa ctttatctgc tacagggtca
    13621 aggaaagcac caaaccgggt actctggttc cagcggcgtg ccagaaaacc atcgaaccag
    13681 tcagtcaccg ccgcgacgca gaaaatgagc gcggcggcaa acggcgacca ggtgacaggc
    13741 agataaaaga ccaatacaaa gaatgggata aggatgacac ggaacagtgt aagcaacgta
    13801 gggatattaa attgcataat gacgggtaac tatctgttgt cagtaagatt acccctatgt
    13861 tgctacagag acatcaatgt ttcaacgacc agaagatctt ttctgccaga ccttgcgaaa
    13921 tacccggcac ttttgcaatt tcctcgacgc tggcgttacg taaaccttgc aaaccgccca
    13981 tatatttcaa caacatttgc cgacgttttg gcccgacgcc ttcaatggtt tccagggaac
    14041 tggtattttt gaccttcgcc cgttttttac ggtgcccgcc aatcgcgtga tcatgtgatt
    14101 catcgcgaat atgctggata acatgcagcg cgggagaatc tggcggcaaa ctgaatccct
    14161 caccttccgg ctcaaagaac agcgtttcca gcccagcctt acgatctgct cctttggcaa
    14221 cgccaagtag cagcggatga tttttatccc atgagacatc cagttcggcg aagacatttt
    14281 tcgcctgcgc aagctggcct ttgccgccgt cgataaggat cacatccggg atcttactgt
    14341 cgtcaatggc tttaccataa cgccgacgca gcacctgatt catcgccgca taatcatcgc
    14401 ccggcgtgat gcctgtaatg ttatagcgcc gatactccgc acgcagcggg ccgttagcat
    14461 caaacaccac acaggaagcg acggtttgtt cgcccatggt atggctgatg tcaaagcact
    14521 ccatccgctt cacttccggc aatttcaaca cgctggcaag cgcggtcagt cgctggtgaa
    14581 cggtagattg ctgcgaaagt ttgctggtta aggccgtcgc cgcattggtg cgcgcgagtt
    14641 tcagataacg cgccctatcg ccgcgaggtt tggtttgaac attaatcttg cgtcccgcca
    14701 gttctgaaag ggaatcggcg agcagcgttt tatcgctaag attaaaatcg agcaggatct
    14761 cacccggtaa ggtgcgcatc tggctgcctt gtaaatagaa ctggcctacg aaggtttcta
    14821 ccacctcgct cagttccgta ccgccaggca ctttcgggaa atagctgcgg ctgccgagca
    14881 ctttgccctg acgaatgaac aatacgtgga cacaagccat gcccgcatcg aacgccacac
    14941 caataacgtc gaggtcgtcg ccggtattgg aaacgaattg tttttcggtg acgcgtcgca
    15001 ccgcctgaat ttggtcgcga atacgtgcag cttcttcaaa ctccagattc tggctggcag
    15061 tttccatacg actaatgagt tgcgtaagca cctgatcatc tttgccagac aaaaacaggc
    15121 gcacatactc aacctgctga gcgtattctt cttcactcac cagtccttca acgcacggtc
    15181 ccagacagcg ccctatctga tattgcagac acggacgcga gcgattgcga taaacactat
    15241 tttcgcactg gcgaatgggg aaaatctttt gcagtagcgc cagtgtttca cgtacggcat
    15301 agccattcgg gaacgggccg aaatattcac ctttggcatg cttcgcacca cgatgcatcg
    15361 ccagacgcgg gtgggtatcg ccactcagga agataaaagg ataagattta tcatcgcgta
    15421 gcaaaacgtt gtaacgcggc tgatagagtt tgatgtagtt gtgttccagc aacagcgctt
    15481 cggtttctgt atgcgtaacc gttacatcaa tttgctggat ctgggcgacc agcgcttcgg
    15541 ttttgcgcga agcgaggttg ctgcggaaat agctggaaag ccgttttttc aggtctttcg
    15601 ctttgccgac atagataacc gtaccaccag catcgtacat gcgataaacg cctggctggc
    15661 aggttacggt ttttaaaaac gcttttgcgt caaactgatc actcactgac ttgataatgt
    15721 ctccgcatta cacagaccat ggcgaattgc caggtgagtc agctcaacat cgccatgaat
    15781 gtttagttta ctgaacatac gatagcggta gctgttcacc gttttcggac tgagattgag
    15841 ctgttctgag atctcattga ccttctggcc cttggtgatc atcagcataa tctgcaattc
    15901 acgttcagac aaactggcaa atgggctttc tgttttttct ggttcgatct ggcttaacgc
    15961 catttgttga gcgatatcag aagcaatgta acgctgccct gaatagacag aacgaatcgc
    16021 actcacgact tcctgcggag ccgcgccttt gctgaggtag cccgcagcac cggcctgcat
    16081 gactttcgct ggtaaagggt tttctgtatg gacggtaagc atgatgattt tgacatcagc
    16141 tgtggaacgc gcgattttac gcgtcgcctc aagaccgcca atgcccggca tactcatgtc
    16201 cattagcacc acgtcaacgg catttgtccg gcaccactta acggcgtctt caccgcacga
    16261 tgcctcaccg acgactttta taccctttat atcttccaga atgcgtcgta tccctgcgcg
    16321 caccagttcg tggtcatcaa caagtagaac gttgatcaaa ggaatatctc cagaaatagg
    16381 gataacgcta ctgatagtta gtcattcgta tattagcggt ttttattgca actttgaaac
    16441 gttaaaaatg gtgggtattc gatttttctc tcgtttttgt aaattcgatt gtccacagtt
    16501 atggtcacgc ccgtcaccaa cggcttccgg gatcgtttca cagtaagccc ggcgatgaaa
    16561 tgtgacaaaa attgacttta ttcagcaaaa atgaaaatca gcccgctgaa tatgtaacat
    16621 taattaaccc gaacaatacg ggcaaaaaac attcatgata aaaatattta ttggtcatta
    16681 tattaacgca tttaatagca ttgttgacgt cacgctcaaa aaacaaccac tgctattttt
    16741 agcaaagctt atggtatact ccgccgcctt aacatttttc accgcaaatt ttcattgcaa
    16801 catgacgagg aaaataaatg agtacgcctg atttttctac tgccgagaat aatcaagaac
    16861 tggcaaatga agtctcctgc ctgaaagcga tgctgacgct gatgctacag gcgatgggac
    16921 aagctgacgc gggccgcgtg atgttaaaga tggaaaaaca gcttgcgctg atcgaagacg
    16981 aaacccaggc tgcagtattt tccaaaacgg ttaagcaaat taaacaagcc taccgtcagt
    17041 aatataaaac cggctgagag cgtgcctttc agccgatttt tgcatctggc acgcagaaag
    17101 aaaagcgatc aaattaagcc agtagcggcc gcgtaacagg ccacctgggt cttatttggt
    17161 gcattgattt ttttctgcat gtttttctga tggaaattga ccgtattctc agagattgac
    17221 aaaatcatcg ctatctctgc tgatgtcttc ccttccgccg tccatttcag aatttctttt
    17281 tcgcgcttgc tgaaattcat ctctggcgtc atcactattt catcatttaa acgcatcaga
    17341 gccatcagac tttcgcgcac cagtaactgc atttttaatt gcagttcatc actaagaatg
    17401 ggtatttcgc gcgtgctgca acgggaaaag gacaaaaagc ccagcgcccg atttggcagc
    17461 attaaatact gagtgacacc gcggcgtaaa ccatgtgcgt gcgcggcttc ccataatggc
    17521 tgtgcttcgc tgaataagtc atcattccac attaaatggc cctgactaaa gttttcaggg
    17581 ttcagcacag gatcaattgc gagaaaattt tttgcctgat aataactaac ccacgactca
    17641 gggtaattgg tgtagaaagc cactttaggt cgcgtgaatg gtaccgggtg gcgaacacat
    17701 aacgaatagt aatcgtactc cagctgctga gcctgaagct ctatttcatg gtagacctct
    17761 tctgcggcct ccatcctctg aaaacgcaac agcatcgtgc gacgccagct gaaaaaatcc
    17821 gtatcctgca tagtaaaccg caacgcccct gagagtgagt atcatttata atgataattc
    17881 aaactatcac ataaataaga tttatatata atttatatta ttcaggcaat gaattacttt
    17941 tgcaagccat cgcattctct tatgttatta atgagttatg ctgatttgtt aagcagtttt
    18001 atcacgcttg aaatggcgtc ccgccccgac aggtgaatcg tcggggctga ttttttctta
    18061 ttattgcagt agaaacttct cgaggaactg gcgggtgcga ggctgctggg gatcagcaaa
    18121 taacgctttt gctgccccct gctcgactat ccgcccctgg tccataaaaa tcgcccggtc
    18181 cgcaacatcc cgggcaaagc tcatttcgtg cgtcacaatc accatcgtgc gcttttcctg
    18241 cgccagctgg cggattgtgt tcaggacttc gcccaccagc tctggatcca gcgctgacgt
    18301 tggctcgtca aacaaaatca cctcaggacg cattgccagc gcacgcgcaa tcgctacacg
    18361 ctgctgttga ccgccagaca aacgacgtgg atagctggtt tctttaccgg ccagcccaac
    18421 tttcgccagc agctcgcgag cgcgcgccgt ggcctcttct ttcggttcgc ctttgacgat
    18481 caccggccct tcaataatgt tttccagcac cgtacgatgc ggaaacaaat taaagttctg
    18541 gaagacaaac ccgacgtgct gacgcaactg gcggatcagc gatttttgct gacttaatga
    18601 acgtgcagta tcaatagtga tatcgccaac ggtgatcgtt cccgcttccg gttgttccag
    18661 cagatttatg ctgcgtagca acgtggtttt gccggaacca ctcggaccga taattgccac
    18721 cacttcgcca ggctttacct cgaggtcgat accgtgcagc accgtctgac cgtgaaattt
    18781 tttcaccagg ttcttaactt caatggcact cattttggtt ctctctcctg gcgattaagt
    18841 tgattctcaa aatggttctg caacgtcgat aatactgtcg ccatgatcca gtagatgagc
    18901 gaagccgcca ggtacatggt gaacacctcc agcgtgcgcg aggtaatcaa ctgcgcctga
    18961 cggaacagct ccggcacctg gattgtcgcg gccagcgacg tatcctttac caggctgata
    19021 aagctgttcg acagcggcgg caacgccacc cgcgccgcct gtggcaaaat cgcgcgacgc
    19081 atagtctgcc atggcgtcat accaatactg gcagctgctt cccactgacc tttatcaata
    19141 gaagaaatgg cggcgcgcag tgtttcggcg gcataggcgg cagtgttcag cgacagacca
    19201 atcattgctg acggaatggg atctaattcg ataccaaact gcggcaggcc gtaatagatc
    19261 ataaacagtt gtgcgatgag cggtgtacca cgaaaaatag agatataaaa acgcgccagc
    19321 cagcgcacgg gccagattgg cgacaggcgc atcagcgcga gaataaaccc gagcaataag
    19381 ccaaaaaaca tgccgccgat actgagttgc agcgtatacc cagccccttt cagcaggaac
    19441 ggcaaggaat caataaccag ttgtatactt tcttgcatga gcgttcccgg agctaaacgt
    19501 ggggatgata ggcgaacagc gcaggcgcac cgccggtatg aataaacaga atcggccctt
    19561 catctttgaa gcgtttctga ctgatacagc caatcagccc cgccatcgct tttccggtat
    19621 acacaggatc aagcagaatg ccttccagcc gcgccagcaa tttcactgct tccatgcctt
    19681 cgtcgttcgg cacgccgtag ccaggtgcaa aatagtcatc ccagagtata atttccgctg
    19741 atgcggtcag ctccagttct ttcgcaatcg cctgttgtag gttaaccact ttcggcaact
    19801 gatcggcaac ggaacgcgac acggtcacgc caatcagttc gctttcaggc atcaggtgtt
    19861 ccagcccaac agccagtccg gcgtgagttc cggcactgcc cgatgcgact accaccgacg
    19921 aaatattaac cgccccttca cactgttgcg cgatttccag cgcactctcc acataaccta
    19981 gcgcgccaag agcattagaa ccgccaaccg gaatgacata cgggcgaaag ccttgtgctt
    20041 cgactcgcgt cgccagctct tccagttggg cattgggatc ggtcagtgct tcgcacattt
    20101 caatctgggt attgaacaga tccagcaaca agcgattgcc gttggttaaa tagttttctg
    20161 cggttgtgcc aataggattt tccagcagcg ccacacagtg cagaccgagt ttcgccgcaa
    20221 ctgcggcagt ctgccgcaca tggttagact ggatcacccc ggcagtaatc agcgtatcgg
    20281 caccttcgcg cagagcatct gccgcgagaa attctagctt acgtaattta ttgccgccca
    20341 ttgccatggg ggtgacgtca tcccgtttga tgaaaatttc ccgtcctaga taatcagaaa
    20401 agcgcggcag atattcgagc ggcgttggcg cgccgataaa ctccaaccgt ggaaaacggg
    20461 ttaaattatg cagtggcata acagcctccg atgtatgttg ttgtgatttt cttattatgc
    20521 acgctgaaaa cgcgtaaata aaaaagg
//
LOCUS       DADMKF010000068        20148 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.069, whole genome shotgun sequence.
ACCESSION   DADMKF010000068 DADMKF010000000
VERSION     DADMKF010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20148
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.069"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(<1..1278)
                     /locus_tag="JNC24_002256"
     CDS             complement(<1..1278)
                     /locus_tag="JNC24_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001324426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9158993.1"
                     /translation="MEYFMKGKSALTLLLAGIFSCGTCQATGAEVTSESVFNILNSTG
                     AATDKSYLSLNPDKYPNYRLLIHSAKLKNEIKSHYTKDEIQGLLTLTENTRKLTLTEK
                     PWGTFILASTFEDDKTAAETHYDAVWLRDSLWGYMALVSDQGNSVAAKKVLLTLWDYM
                     STLDQIKRMQDVISNPKRLDGVPGQMNAVHIRFDSNSPVMADVQEEGKPQLWNHKQND
                     ALGLYLDLLIQAIDTGTINAEDWQKGDRLKSVALLIAYLDKANFYVMEDSGAWEEDAR
                     LNTSSVALVTSGLERLSNLLSKKDSVFVSDLLREAKANELDEPLSTTRLNHLIDKGYE
                     RITLQLDLGGESPGYLEKDKHYREADAALLNVIYPANLAKINTRRKEQVLKIVKKLAG
                     PYGIKRYEKDNYQSANFWFNDIKTDTDQNSHAKR"
     gene            complement(1471..1540)
                     /locus_tag="JNC24_002257"
     tRNA            complement(1471..1540)
                     /locus_tag="JNC24_002257"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1506..1508),aa:Gly,seq:ccc)
     gene            complement(1619..2374)
                     /locus_tag="JNC24_002258"
     CDS             complement(1619..2374)
                     /locus_tag="JNC24_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAY9158994.1"
                     /translation="MSAGRLNKKSLGIVMLLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            2789..5086
                     /gene="xdhA"
                     /locus_tag="JNC24_002259"
     CDS             2789..5086
                     /gene="xdhA"
                     /locus_tag="JNC24_002259"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HAY9158995.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAAQLVSIEWQELPVITTPEAALAEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFVGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYASHGHSIASAGRN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRIYSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFAPGAFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPSAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            5097..5975
                     /gene="xdhB"
                     /locus_tag="JNC24_002260"
     CDS             5097..5975
                     /gene="xdhB"
                     /locus_tag="JNC24_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HAY9158996.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLADGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEAPITQRHLPALCAAATSIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGSAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            5972..6451
                     /gene="xdhC"
                     /locus_tag="JNC24_002261"
     CDS             5972..6451
                     /gene="xdhC"
                     /locus_tag="JNC24_002261"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HAY9158997.1"
                     /translation="MNHSETITIECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGAVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(6491..8269)
                     /locus_tag="JNC24_002262"
     CDS             complement(6491..8269)
                     /locus_tag="JNC24_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HAY9158998.1"
                     /translation="MELATTQSVLMQIQPTIQRFARMLASVLQFEVEIVDENLCRVAG
                     TGAYGKFLGRQLSGNSRLLRHVLETKTEKVVTQSRFDPLCEGCDSKENCREKAFLGTP
                     VILQDRCVGVISLIAVTHEQQEHISDNLREFSDYVRHISTIFVSKLLEDQGPGDNISK
                     IFATMIDNMDQGVLVVDDESRVQFVNQTALKTLGVVQNNIIGKPIRFRPLTFESNFTH
                     GHMQHIVSWDDKSELIIGQLHNIQGRQLFLMAFHQSHTSFSVANAPDEPHIEQLVGEC
                     RVMRQLKRLISRIAPSPSSVMVVGESGTGKEVVARAIHKLSGRRNKPFIAINCAAIPE
                     QLLESELFGYVKGAFTGASANGKTGLIQAANTGTLFLDEIGDMPLMLQAKLLRAIEAR
                     EILPIGASSPIQVDIRIISATNQNLAQFIAEGKFREDLFYRLNVIPITLPPLRERQED
                     IELLVHYFLHLHTRRLGSVYPGIAPDVVEILRKHRWPGNLRELSNLMEYLVNVVPSGE
                     VIDSTLLPPNLLNNGTTEQSDVTEVSEAHLSLDDAGGTALEEMEKQMIREALSRHNSK
                     KQVADELGIGIATLYRKIKKYELLNT"
     gene            8748..9935
                     /gene="ygeW"
                     /locus_tag="JNC24_002263"
     CDS             8748..9935
                     /gene="ygeW"
                     /locus_tag="JNC24_002263"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HAY9158999.1"
                     /translation="MKTVNELIKDINSLTSHLHEKDFLLTWEQTPDELKQVLDVAAAL
                     KALRAENISTKVFNSGLGISVFRDNSTRTRFSYASALNLLGLAQQDLDEGKSQIAHGE
                     TVRETANMISFCADAIGIRDDMYLGAGNAYMREVGAALDDGYKQGVLPQRPALVNLQC
                     DIDHPTQSMADLAWLREHFGSLENLKGKKIAMTWAYSPSYGKPLSVPQGIIGLMTRFG
                     MDVTLAHPEGYDLIPDVVEVAKNNAKASGGSFRQVTSMEEAFKDADIVYPKSWAPYKV
                     MEERTELLRANDHEGLKALEKQCLAQNAQHKDWHCTEEMMELTRDGEALYMHCLPADI
                     SGVSCKEGEVTEGVFEKYRIATYKEASWKPYIIAAMILSRKYAKPGALLEQLLKEAQE
                     RVK"
     gene            9993..11189
                     /gene="dpaL"
                     /locus_tag="JNC24_002264"
                     /pseudo
     CDS             9993..11189
                     /gene="dpaL"
                     /locus_tag="JNC24_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311771.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
     gene            11247..12458
                     /locus_tag="JNC24_002265"
     CDS             11247..12458
                     /locus_tag="JNC24_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HAY9159000.1"
                     /translation="MAKNIPFKLILEKAKDYQADMTRFLRDMVAIPSESCDEKRVVHR
                     IKEEMEKVGFDKVEIDPMGNVLGYIGHGPRLVAMDAHIDTVGIGNIKNWDFDPYEGME
                     TDELIGGRGTSDQEGGMASMVYAGKIIKDLGLEDEYTLLVTGTVQEEDCDGLCWQYII
                     EQSGIRPEFVVSTEPTDCQIYRGQRGRMEIRIDVQGVSCHGSAPERGDNAIFKMGPIL
                     GELQELSQRLGYDEFLGKGTLTVSEIFFTSPSRCAVADSCAVSIDRRLTWGETWEGAL
                     DEIRALPAVQKANAVVSMYNYDRPSWTGLVYPTECYFPTWKVEEDHFTVKALVNAYEG
                     LFGKAPVVDKWTFSTNGVSIMGRHGIPVIGFGPGKEPEAHAPNEKTWKSHLVTCAAMY
                     AAIPLSWLATE"
     gene            12511..13895
                     /gene="hydA"
                     /locus_tag="JNC24_002266"
                     /pseudo
     CDS             12511..13895
                     /gene="hydA"
                     /locus_tag="JNC24_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311773.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
     gene            13943..14875
                     /gene="arcC"
                     /locus_tag="JNC24_002267"
     CDS             13943..14875
                     /gene="arcC"
                     /locus_tag="JNC24_002267"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HAY9159001.1"
                     /translation="MSKKIVLALGGNALGDDLAGQMKAVKITSQAIVDLIAQGHEVIV
                     THGNGPQVGMINQAFEAAAKTEAHSPMLPMSVCVALSQGYIGYDLQNALREELLSRGI
                     NKPVATLVTQVEVDANDPAFLNPTKPIGSFFTEQEAEQLTKQGYTLKEDAGRGYRRVV
                     ASPKPVDIIEKETVKALVDAGQVVIAVGGGGIPVIREGNHLRGASAVIDKDWASARLA
                     EMIDADMLIILTAVEKVAINFGKENEQWLDRLSLSDAERFIEEGHFAKGSMLPKVEAA
                     ASFARSRAGREALITVLSKAKEGIEGKTGTVICQ"
     gene            complement(14916..16541)
                     /locus_tag="JNC24_002268"
     CDS             complement(14916..16541)
                     /locus_tag="JNC24_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF2563 family selenium-dependent molybdenum
                     hydroxylase system protein"
                     /protein_id="HAY9159002.1"
                     /translation="MNIFTEAAKLEEQNCPFAMAQIVDSRGSTPRHSAQMLVRADGSI
                     VGTIGGGMVERKVIEESLQALQERKPRLFHGRMARNGADAVGSDCGGAMSVFISVHGM
                     RPRLVLIGAGHVNRAIAQSAALLGFDIAVADIYRESLNPELFPPSTTLLHAESFGAAV
                     EELDIRPDNFVLIATNNQDREALDKLIEQPIAWLGLLASRRKVQLFLRQLREKGVAEE
                     HIARLHAPVGYNIGAETPQEIAISVLAEILWVKNNAPGGLMMKPSHPSGHQLVVIRGA
                     GDIASGVALRLYHAGFKVIMLEVEKPTVIRCTVAFAQAVFDGEMTVEGVTARLATSSA
                     EAMKLTERGFIPVMVDPACSLLDELKPLCVVDAILAKQNLGTRADMAPVTIALGPGFT
                     AGKDCHAVIETNRGHWLGQVIYSGCAQENTDVPGNIMGHTTRRVIRAPAAGIMRSNVK
                     LGDLVKEGDVIAWIGEHEIKAPLTGMVRGLLNDGLAVVGGFKIGDIDPRGETADFTSV
                     SDKARAIGGGVLEALMMLMHQGVKATKEVLEVA"
     gene            complement(16589..17359)
                     /gene="yqeC"
                     /locus_tag="JNC24_002269"
     CDS             complement(16589..17359)
                     /gene="yqeC"
                     /locus_tag="JNC24_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium-dependent hydroxylase
                     accessory protein YqeC"
                     /protein_id="HAY9159003.1"
                     /translation="MKSIVDPSALVIDLGAQKRPTVISVVGAGGKTSLLFWLAELLQA
                     SGRRVLITTTTHMFMPTSHWPVVFCRDPAMLPHASLTSPISFCFHSWKANQGKVQGFT
                     PEAIDALVQRPECDVILIEADGSRGMPLKAPDEHEPCIPKSSCCVIAVMGGHTLGAKV
                     STENVHRWSQFADITGLTPDATLQLSDLVALVRHPQGAFKNVPQGCRRVWFINRFSQC
                     ENAIAQSELLQPLQQHDVEAIWLGDIQEHPAIARRFVN"
     gene            17462..18040
                     /gene="mocA"
                     /locus_tag="JNC24_002270"
     CDS             17462..18040
                     /gene="mocA"
                     /locus_tag="JNC24_002270"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HAY9159004.1"
                     /translation="MSAIDCIITAAGLSSRMGQWKMMFPWEQGTILDTSIKNALQFCS
                     RIILVTGYRGNELHERYANQSNITIIHNPDYAQGLLTSVKAAVPAVQTEHCFLTHGDM
                     PTLTIDIFRKIWSLRNDGAILPLHNGIPGHPILVSKPCLMQAIQRPNVTNMRQALLMG
                     DHYSVEIENAEIILDIDTPDDFITAQKRYTKI"
     gene            18362..>20148
                     /gene="ygfK"
                     /locus_tag="JNC24_002271"
     CDS             18362..>20148
                     /gene="ygfK"
                     /locus_tag="JNC24_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate reductase subunit YgfK"
                     /protein_id="HAY9159005.1"
                     /translation="MGDIMRPIPFEELLTRIFDEYQQQRSIFGIPEQQFYSPVKGKTV
                     SVFGETCATPVGPAAGPHTQLAQNIVTSWLTGGRFIELKTVQILDRLELEKPCIDAED
                     ECFNTEWSTEFTLLKAWDEYLKAWFALHLLEAMLQPSDSGKSFIFNMSIGYNLEGIKQ
                     PPMQQFIDNMMDASDHPKFAQYRDTLNKLLQDDAFLARHGLQEKRENLQALPARIPTS
                     MVQGVPLSTMHGCPPHEIEAICRYMLEEKGLNTFVKLNPTLLGYARVREILDVCGFGY
                     IGLKEESFDHDLKLTQALEMLERLMVLAKEKSLGFGVKLTNTLGTINNKGALPGEEMY
                     MSGRALFPLSINVAAVLSRAFDGKLPISYSGGASQLTIRDIFDTGIRPITMATDLLKP
                     GGYLRLSACMRELEGSDAWGLDHVDVERLNRLAADALTMEYTQKHWKPEERIEVAEDL
                     PLTDCYVAPCVTACAIKQDIPEYIRLLGEHRYADALELIYQRNALPAITGHICDHQCQ
                     YNCTRLDYDSALNIRELKKVALEKGWDEYKQRWHKPAGSGSRHPVAVIGAGPAGLAAG
                     YFLARAGHPVTLFEREANAGGVVKNIIPQ"
BASE COUNT         5224 a         5179 c         4765 g         4980 t
ORIGIN      
        1 tcttttcgca tgtgaatttt gatcggtatc ggttttaata tcattgaacc aaaaatttgc
       61 cgactgataa ttatccttct cgtatctttt tataccatac ggacctgcta gttttttaac
      121 tatttttaga acctgctctt ttcttctggt gtttatcttt gcaagatttg caggataaat
      181 aacattaagt aaagccgcat cggcttctct gtaatgttta tctttttcca gataacctgg
      241 ggattctccc cctaaatcaa gctgtaacgt tattctttca taacctttat ctatgaggtg
      301 gttcaacctc gttgttgaaa gaggttcatc cagttcattg gctttagctt cgcgtagtaa
      361 atcagagaca aaaacagagt ctttcttcga gagtaagttc gacaaacgtt ccagtccaga
      421 ggtcaccagt gcaacggaag atgtgtttaa tcgggcatct tcttcccatg ctccggaatc
      481 ttccataaca tagaaattgg ctttatccag ataagcaatc agcagcgcaa ccgattttaa
      541 tcggtcacct ttttgccaat cttcagcgtt aattgtacca gtgtcgattg cttgtataag
      601 gagatcaaga taaagaccta acgcatcatt ttgtttatgg ttccacagct gcggcttgcc
      661 ttcttcctgc acatctgcca tcacggggga gttgctgtca aatcggatat gcacagcatt
      721 catttgcccg ggaactccat ccagtcgttt ggggttgcta atgacgtcct gcatccgctt
      781 aatctggtcg agcgtagaca tataatccca cagggtgagc agaacctttt ttgcggctac
      841 actatttccc tgatctgata ctaatgccat atagccccat aaactatccc tcagccagac
      901 agcatcatag tgggtttctg ccgctgtttt gtcatcttcg aaagtagaag caagaataaa
      961 tgttccccag ggtttctccg tcaacgttaa ctttcgtgtg ttttctgtta atgttaacaa
     1021 cccttgaatt tcgtctttgg tatagtggct ctttatttca ttttttaatt tggctgagtg
     1081 aatcagaaga cgataattag ggtacttatc tggatttaac gataaataac ttttatctgt
     1141 tgctgccccc gtggaattaa gaatgttaaa tacagattca ctcgtcactt ctgcccctgt
     1201 cgcctggcat gtgccgcacg aaaatatacc agcgagcaga agagttaatg cagatttccc
     1261 tttcataaaa tattccatca atcgtttgtt aaaagcgtaa ataaccactt atttaacaac
     1321 ttgaaaataa ggtgttttac ctgggttgtt acaaaaggat tgcattgcgt aaacgctttt
     1381 tatttacaac aaaatgggga aatattacgg cgattgcgaa aagtctgaag cgattgaatt
     1441 aagataatat agaaaatcag atacagcaaa agcggcgaag ggaatcgaac cctcgtatag
     1501 agcttgggaa gctctcgttc taccattgaa ctacgcccgc ttcgagatgc gtaaggcatt
     1561 ataaacctta cgctctcctt agcaagtgcc gcgctgctga ctgctgatta attcgccatc
     1621 agcattttgg cttgctgccc tgcggcggca agtaacgtag cggatcaatt gccgttgcac
     1681 ggtaacgaat ctggaaatgc aggcgaacag atgccgcatc cgtgctcccc atagtggcga
     1741 ttttttgccc agccttcacg ctttgcccat tatttaccag catcgtgtca ttatgggcgt
     1801 aagccgtaat gtaatcttca ctgtgtttaa tcatgatgag attaccgtag ccacgcagct
     1861 ggttgcccac atacaccacc tttcctgcac ccgcggcgta aataggtgta ccccgtggag
     1921 ctgagatatc aatcccttta ttgccgccat ctgctgtcga atacggcatg ataactttcc
     1981 ctgtcgttgg ccataaccaa caacgttgcc ctactggcgg ccaggatgat ttcggtaccg
     2041 ctgatgacgg tgtaaccgat gcggttttgg tcgttgattt ggcggttgat ttacgtgtac
     2101 tactgctact tttcgcccca cccagtttta gtttctgacc aacttcaatg gtgtaagggg
     2161 gggaaatgcc gttcagtcgc gccagttctt ttacgctggt tcccgtggtg cgcgaaatac
     2221 gatatagcgt atccccccgt ttcacggtgt aaacggagcc ggaatacgtt cctgtatcgg
     2281 atgatttgct acccgaacag cccgccaaaa gcagtccaac cgataacaac atcacgatac
     2341 ccagagattt tttattcagg cgtcccgcac tcaaaatctt tcctcgctaa aaaaacaaga
     2401 cgccttatca tagcagccag cctcttgata cccaatctct tgcttctgga aaaacgtggt
     2461 aatagagaaa gaggttaatt tcatttggca aagggaagta tcatcagcaa aatattcgct
     2521 gtagaggtat cgaacttcct gactttttac cctatttccc atcctgatgc gtaataatcc
     2581 caatttatca cattgatttt atcgccactc atattgatac gtatcactgc ccctattcag
     2641 agtcctgttt cgtatcatat ctgtgttttt ttaacgtcat cacacttcct acctccccta
     2701 acgcttatcg tcgtttctgg cgtaaatctt gcctgcttag actaaatctt tgccataaga
     2761 accaaatgtt caaatcgagg ggatttctat ggaagcgcgg gaagcaaccg ctacgggtga
     2821 atcatgcatg cgcgtcgatg ccattgctaa ggtcaccggg cgggcacgat atactgacga
     2881 ttatgttatg gcgggcatgt gttatgcgaa atatgtacgt agccctatcg cacatggtta
     2941 tgccgtaagt attaatgatg aacaagccag aagtttgccg ggcgtactgg cgatttttac
     3001 ctgggaagat gtgcctgata ttccattcgc tacagctggg catgcctgga cacttgacga
     3061 aaacaagcgc gataccgccg atcgcgcact gctaactcgc catgttcgtc atcatggtga
     3121 cgccgttgcc atcgtcgtgg cccgcgatga actcacggca gaaaaagcgg cgcaattggt
     3181 cagcattgag tggcaagaat tacccgttat caccacgcca gaagcggcgc tggcagaaga
     3241 cgctgcacca atccataacg gtggcaattt actgaaacaa agcacgatgt cgacgggtaa
     3301 tgtccaacaa acaatcgatg ccgccgacta ccaggtacag gggcactatc agacccccgt
     3361 tattcaacat tgtcacatgg aaagcgtaac atcgctggcg tggatggagg atgactcgcg
     3421 aattaccatc gtttccagca cccagatccc gcacattgtt cgccgcgtgg ttggtcaggc
     3481 gctggatatt ccctggtcat gcgtacgagt catcaaaccg tttgtcggtg gcggttttgg
     3541 taataaacag gatgtactgg aagagccaat ggcagcattc ctgaccagca agcttggcgg
     3601 cattccggtg aaagtttccc ttagccgtga agagtgtttc ctcgcaaccc gtacccgcca
     3661 cgcttttacc attgacgggc aaatgggcgt gaaccgcgac ggaacattga aaggttatag
     3721 tctggatgtt ctgtctaaca ccggcgctta tgcatctcac gggcactcca tcgcttctgc
     3781 ggggaggaat aaagtcgctt acctttatcc tcgttgtgcc tacgcttaca gttcaaagac
     3841 ctgctatacc aacctcccct cggctggtgc gatgcgtggt tatggcgcgc cacaagtcgt
     3901 atttgccgtt gagtctatgc ttgatgacgc cgcgacagcg ttaggtattg atcctgttga
     3961 aattcgttta cgcaacgccg cccgcgaagg agatgctaat ccgctcacgg gcaaacgtat
     4021 ttacagcgca gggttgccgg agtgtcttga aaaaggccgg aaaatctttg aatgggaaaa
     4081 acgccgtgca gaatgccaga accagcaagg caatttgcgc cgcggcgttg gcgtcgcctg
     4141 ttttagctat acctctaaca cctggcctgt cggcgtagaa atagcaggcg cgcgccttct
     4201 gatgaatcag gatggaacca tcaacgtgca aagcggcgcg acggaaatcg gtcagggtgc
     4261 cgacaccgtc ttctcgcaaa tggtggcaga aaccgtgggg gttccggtca gcgacgttcg
     4321 cgttatttca actcaagata ccgacgttac gccgttcgct cccggcgcat ttgcctcacg
     4381 ccagagctat gttgccgcgc ctgcgctgcg cagtgcggca ctattattaa aagagaaaat
     4441 catcgctcac gccgcagtca tgctacatca gtcagcgatg aatctgaccc tgataaaagg
     4501 ccatatcgtg ctggttgaac gaccggaaga gccgttaatg tcgttaaaag atttggcgat
     4561 ggacgctttc taccaccctg aacgcggcgg gcagctctct gctgaaagct ccatcaaaac
     4621 caccactaac ccatcggcgt ttggctgtac ctttgttgat ctgacggtcg atattgcgct
     4681 gtgcaaagtc accatcaacc gcatcctcaa cgttcatgat tcagggcata ttcttaatcc
     4741 actgctggca gaaggtcagg tacacggcgg aatgggaatg ggcattggct gggcgctatt
     4801 tgaagagatg atcatcgatg ctaaaagcgg cgtggtccgt aaccccaatc tgctggatta
     4861 caaaatgccg accatgccgg atctgccaca actggaaagc gcgttcgtcg aaatcaatga
     4921 gccgcaatcc gcatacggac ataagtcact gggtgagcca ccaataattc ctgttgccgc
     4981 tgctattcgt aacgcggtga agatggctac cggtgttgca atcaatacac tgccgctgac
     5041 gccaaaacgg ttatatgaag agttccatct ggcaggattg atttgaggat aacatcatgt
     5101 ttgattttgc ttcttaccat cgcgcagcaa cccttgccga tgccatcaac ctgctggctg
     5161 acaacccgca ggccaaactg ctcgccgatg gcactgacgt actgattcag ctccaccatc
     5221 acaatgaccg ttatcgccat attgttgata ttcataatct ggcggagctg cggggaatta
     5281 cgctggcgga agatggctcg ctacgtatcg gctctgcaac gacatttacc cagctaatag
     5341 aagctcctat aactcaacgt catctcccgg cgttatgtgc tgcggccacg tccattgctg
     5401 gaccgcagat ccgtaacgtc gctacctacg gtggaaatat ttgcaacggt gccaccagcg
     5461 cagattctgc cacgccaacg ctaatttatg acgcgaaact ggagatccac tccccgcgcg
     5521 gtgttcgttt cgtcccgatt aatggctttc acaccgggcc gggcaaagtg tctcttgagc
     5581 atgacgaaat cctcgtcgcc tttcattttc cgccacagcc gaaagaacac gcgggcagcg
     5641 cgcattttaa atatgccatg cgcgacgcaa tggatatttc aacgattggc agcgccgcac
     5701 attgccgact ggataacggc aatttcagcg aattacgcct ggcatttggt gttgccgcgc
     5761 caacgccgat tcgctgccaa catgccgaac agactgcaca aaatgcgcca ttaaacctgc
     5821 aaacgctgga agctatcagc gaatctgtcc tgcaagatgt cgccccgcgt tcttcatggc
     5881 gggccagtaa agagtttcgt ctgcatctca tccagacgat gaccaaaaaa gtgattagcg
     5941 aagccgtcgc cgcggcgggg ggaaaattgc aatgaatcac agcgaaacaa ttaccatcga
     6001 atgcaccatt aacgggatgc cttttcagct tcacgccgca ccaggcacgc cgctctcgga
     6061 attactccgc gaacaaggac tgctaagtgt caaacaaggg tgctgcgtgg gtgaatgtgg
     6121 tgcctgtacg gtgttggtcg acggcacagc aatagacagt tgcttatacc ttgccgcctg
     6181 ggctgaagga aaggagatcc gcacgctgga aggtgaagcg aaaggcggaa aactttctca
     6241 tgttcagcag gcttatgcga aatccggcgc agtgcagtgc gggttttgta cgcctggcct
     6301 gattatggct accacggcaa tgctggcgaa accacgcgag aagccattaa ccattacgga
     6361 aattcgtcgc ggactggcgg gaaatctttg tcgctgcacg gggtatcaga tgattgtaaa
     6421 tacagttctg gattgcgaga aaacgaagta aaaggatatc cggcctgaat tcaggccgga
     6481 ttcactgagg ttatgtgttt aacaactcat atttcttaat cttgcgatag agcgtagcaa
     6541 tgccgatgcc cagttcatca gcaacttgct tcttgctgtt atgacgtgaa agcgcctcgc
     6601 ggatcatttg cttttccatc tcctccagcg ccgtgccgcc cgcatcatcg agtgacaggt
     6661 gcgcctcact gacctctgtt acatcacttt gctccgttgt gccattattc agcagatttg
     6721 gcggcaatag cgtgctgtcg ataacttcac ctgaaggaac cacgttaacc agatattcca
     6781 tcaaattgct taactcgcgc aggtttccgg gccaacgatg cttacgcaat atttcgacga
     6841 catcgggagc aatgccagga taaaccgatc ccagacgacg ggtatgcaga tgtaaaaagt
     6901 aatgcaccaa tagttcaata tcttcctgac gttcacgcag cggtggcaga gttatcggga
     6961 taacattaag tcggtagaag agatcttcgc ggaatttacc ttcggcaatg aactgggcca
     7021 aattctgatt agttgcagaa atgatgcgaa tgtcgacttg tattgggcta ctggcaccaa
     7081 tcggcagaat ttcacgcgcc tcaatagcgc gcagtaattt agcctgcaac attaatggca
     7141 tatcacctat ttcatcgaga aacagcgtgc ccgtattcgc cgcctgaatc aaccctgttt
     7201 taccgttggc agaagcacca gtaaatgcgc ctttaacata accgaacagt tcgctttcca
     7261 gaagctgctc cggaatcgcg gcacagttga tagcaataaa gggtttattc cgtcttccgc
     7321 tcaacttatg gattgctcgg gcgacgactt ctttacccgt gccgctttca ccaaccacca
     7381 taacgctgga tgggctgggt gcaatacggc taatgagtcg ttttaattgc cgcataacac
     7441 ggcactcgcc aaccaattgt tcaatatgtg gttcatcagg tgcatttgct acagaaaaac
     7501 tggtatgcga ttggtgaaat gccattaaaa ataattgtcg gccctgaatg ttatgcaatt
     7561 gaccaatgat taattcactt ttatcgtccc acgaaacaat atgctgcata tgtccatgag
     7621 taaaattact ctcaaatgtt aatggtctga aacggatagg tttcccaata atattatttt
     7681 gtacaacacc aagtgttttt aaggcagtct gattaacaaa ctgaacccga ctttcatcat
     7741 caacaactaa tacgccctga tccatattat cgatcatggt cgcgaatatt ttactgatgt
     7801 tatctcctgg cccctgatcc tccagaagtt tcgaaacaaa aatggtggat atatggcgaa
     7861 cataatcaga aaattcgcgt aaattatcac tgatatgctc ttgttgctcg tgggtaacgg
     7921 caatcaaact tatcacccca acacaacgat cctgtaaaat gacaggcgta cccagaaatg
     7981 ctttttcgcg gcaattttct ttactatcgc aaccttcgca aaggggatcg aagcgagact
     8041 gtgtcacaac tttttcagtt ttcgtttcca ggacgtggcg gagcaggcgt gagttgccgc
     8101 tcaactggcg accaagaaac ttcccatacg cgcccgttcc ggcgacgcga cacaagtttt
     8161 catcaacgat ctcaacctca aactgcaaaa cgctggcaag cattctggca aaacgctgaa
     8221 ttgtcggttg aatttgcatc aatactgact gcgtagtagc aagctccata gctttacctt
     8281 ccagacttac ttaaaagtcg atcattgaag acgttgatgg ttcacagatc atgatgatat
     8341 taactcaggc gaaattggct ttgataaaaa cataagattt ttatcatttt ctaatgcaat
     8401 tatggaagag atatcacatt tctatatcaa tatgagaatt acggcggtga gtttatcaaa
     8461 ctgaagagag atagcctgcc cctttatctt atttctgata cttagcagaa aataaataac
     8521 gcgataaaaa aagccaaacg ttttcgtatt ttacaaacaa ccagaagctg gcatcaattt
     8581 gtgaacaacc ccacacatta tccgtcaaat tagtcttttg cagccgcgcg gataattctg
     8641 gcacacttat tgttagtccc aggtatagct atgaaaacac caatcacttt ggcaagtcac
     8701 agtgaaataa accactttgc ctgtcattcc actaccggga ctgtatgatg aaaactgtta
     8761 atgagctgat taaggatatc aattcgctga cctctcacct tcacgagaaa gattttttgt
     8821 taacgtggga acagacgcca gatgaactga aacaagtact ggacgttgcc gcagcattaa
     8881 aagcactgcg tgctgaaaac atctcaacca aagtctttaa tagtggatta ggtatttccg
     8941 tattccgcga caactccacc cgtacccgct tctcttatgc ttccgcgctt aacctgctcg
     9001 gccttgcaca acaagatctc gacgaaggca aatcacaaat cgctcacggc gaaaccgtgc
     9061 gtgaaaccgc caatatgatc tccttctgcg ccgacgctat tggtattcgc gacgatatgt
     9121 atctgggcgc aggcaacgcc tatatgcgtg aagttggcgc tgcacttgat gacggttaca
     9181 agcagggtgt actgccacag cgtccggctt tagtgaacct gcaatgcgat attgaccacc
     9241 cgactcagtc aatggctgac ctcgcgtggt tacgtgaaca ctttggttca ctggaaaacc
     9301 tgaaaggtaa aaaaatcgcc atgacctggg cctactctcc aagctatggc aaaccgctct
     9361 ctgtaccaca aggcatcatc ggtctgatga ctcgcttcgg tatggatgtc accttggccc
     9421 atccggaagg ctacgacctg atcccggatg tggttgaagt ggcgaaaaac aatgctaaag
     9481 cctccggtgg tagcttccgt caggtcacca gcatggaaga agccttcaaa gacgcagaca
     9541 tcgtttatcc gaagtcatgg gcaccttaca aagtgatgga agagcgtact gaattgctgc
     9601 gtgcgaacga ccacgaaggc ttaaaagcac tggaaaaaca gtgtctggca cagaacgcgc
     9661 aacacaaaga ctggcattgt actgaagaga tgatggaact gacccgtgat ggcgaagccc
     9721 tgtacatgca ctgcctgcca gctgatatca gcggcgtatc ctgcaaagaa ggtgaagtga
     9781 ctgaaggcgt attcgaaaaa taccgtatcg ctacctacaa agaagccagc tggaagcctt
     9841 atatcatcgc cgcgatgatc ctgtcccgta aatacgccaa accaggtgca ctgctcgagc
     9901 aactgctgaa agaagcgcaa gaacgcgtga aataacccct cgggccgatc tgctggtcgg
     9961 ccctctattt ccagaggcca aaaggatagg atatgtccgt tttctcattg aagattgata
    10021 tcgccgataa caaatttttc aacggcgaaa catcaccgct cttttcgcaa agctaggcca
    10081 aactggcgcg ccagttccac cagaaaatag ctggttatcg cccaacaccg ctttgtgcgc
    10141 tggacgatct cgcaaacctt tttggtgtga agaaaattct cgtcaaagac gaatcaaaac
    10201 gattcggtct gaacgccttc aaaatgcttg gcggtgcgta cgccatcgct caattattgt
    10261 gtgaaaaata tcatcttgat atcgaaacgc tgtcatttga gcacctgaaa aatgccatcg
    10321 gcgaaaaaat gactttcgcg accaccaccg acggcaacca cgggcgcggt gtggcgtggg
    10381 cagcacagca actcggacag aatgcggtga tttacatgcc gaaaggttct gctcaggaac
    10441 gcgttgacgc cattctgaac ctcggtgccg agtgcatcgt cacggatatg aactatgacg
    10501 ataccgttcg cctgaccatg caacacgcgc agcagcacgg ctgggaagtg gtacaggaca
    10561 cggcatggga aggttacacc aaaatcccaa cctggatcat gcaaggctac gcaaccctgg
    10621 cagatgaagc cgtcgagcaa atgcgtgaaa tgggcgtaac cccgacgcac gttctgctgc
    10681 aagccggtgt cggagcaatg gccggtggtg tgctgggtta tctggtcgac gtctacagcc
    10741 cgcaaaatct gcacagcatt attgttgaac ctgacaaagc tgactgtatt tatcgctccg
    10801 gcgtcaaagg cgacatcgtc aacgttggcg gtgatatggc caccatcatg gcgggcctgg
    10861 cctgtggcga acctaacccg ctgggctggg aaatcctacg taactgcgcc acccaattca
    10921 tctcctgcca ggacagcgtt gccgcattag gtatgcgcgt gctgggtaat ccgtacggca
    10981 acgacccgcg catcatctcc ggtgaatccg gcgctgtcgg tttgggcgtt ctcgcagcgg
    11041 ttcattatca cccgcaacat caaagcctga tggaaaaact ggcgctggac aaagatgccg
    11101 tagtgctggt tatcagcact gaaggcgaca ccgacgtgaa gcactaccgc gaagttgtct
    11161 gggaaggcaa acacgctgta gcaccttaat caccctattg gaactactcc ctgcaaaacg
    11221 gggagtaaaa aatctggaga aaaataatgg ctaagaatat tccattcaaa ctgattcttg
    11281 aaaaagcaaa agattaccag gcggacatga ctcgcttcct gcgcgacatg gttgctattc
    11341 ccagtgaaag ctgcgacgaa aaacgcgtag tacatcgtat taaagaagag atggaaaaag
    11401 tcggcttcga taaagttgaa atcgacccga tgggcaacgt tctcggttat atcggccacg
    11461 gcccgcgtct ggtggcaatg gacgctcata tcgataccgt cggcattggc aacatcaaaa
    11521 actgggactt cgatccgtac gaaggcatgg aaactgatga gctaatcggt ggtcgcggta
    11581 cttccgacca ggaaggcggc atggcatcta tggtttatgc cggtaaaatc attaaagacc
    11641 tcggtctgga agatgaatat accctgctgg ttaccggtac tgtgcaggaa gaagactgcg
    11701 acggtctgtg ctggcagtac attattgaac aatccggcat tcgcccggaa tttgtggtca
    11761 gtaccgaacc aaccgactgc cagatatacc gtggtcagcg cggtcgtatg gaaattcgta
    11821 ttgatgttca gggtgttagc tgccacggtt ctgcgccaga acgcggtgac aacgccattt
    11881 tcaaaatggg tccgattctt ggcgaattac aagaactctc ccaacgtctg ggttatgacg
    11941 aattcctcgg caaaggcacc ctcaccgttt ctgaaatctt cttcacatcc ccaagccgtt
    12001 gcgctgtagc agatagctgc gccgtctcta ttgaccgccg tctgacctgg ggcgaaacct
    12061 gggaaggcgc gctggacgaa atccgcgccc tgcctgcagt acagaaagct aacgcggttg
    12121 tttctatgta caactacgac cgtccgtcct ggactggcct ggtttaccca accgaatgct
    12181 acttcccgac ctggaaagtg gaagaagatc acttcaccgt taaagcactg gtgaatgcct
    12241 acgaagggct gtttggcaaa gcgccggttg ttgataagtg gaccttctca actaacggcg
    12301 tatctatcat gggccgtcac ggcattccgg tgatcggctt tggcccgggt aaagaacctg
    12361 aagcgcatgc acctaacgaa aaaacctgga aatctcacct ggtgacctgt gccgcgatgt
    12421 acgctgcaat cccgttaagc tggctggcaa cagagtaatt actcttcact tattcccctc
    12481 cggttcgccg gagggttttt ggagtttgct atgcgcgtat tgatcaaaaa cggcactgtc
    12541 gttaacgcag atggacaagc caaacaggat ttgctgattg aaagcgggat tgttcgccag
    12601 ttgggcaaca atatttcgcc gcagctcccg tatgaagaaa ttgatgccac tggctgttac
    12661 gttttccctg gcggcgtgga tgtccatacg catttcaata ttgatgtcgg catcgcgcgc
    12721 agttgtgatg attttttacc ggtacccgcg cagctgcgtg tggcggtaca acaaccatta
    12781 ttgaccatat gggatttggc ccaaacggct gtcggttacg ccatcaactg gaggtttatc
    12841 gtggttatgc cgcccataaa gcggtcatcg attacagctt tcacggtgtg atccagcaca
    12901 ttaatcacgc aatcctcgac gaaatcccga tgatggtcga ggaaggactg agcagtttta
    12961 aactctattt aacctatcaa tacaaactca acgatgacga ggttttgcag gcattacgcc
    13021 gcttgcatga atccggcgcg ctgaccaccg tgcacccgga aaatgatgcg gctatcgcca
    13081 gcaagcgggc ggaatttatc gccgcagggt taaccgcgcc gcgctatcat gccttgagtc
    13141 gccctctgga atgcgaagcg gaagccatcg cccgcatgat taacctggca caaattgccg
    13201 gtaacgcccc gctctatatc gtgcacctgt ctaacggctt aggtctggat tatctgcgtc
    13261 ttgcccgtgc gaatcaccag ccagtctggg ttgaaacctg cccacaatat ctcctgttgg
    13321 acgaacgcag ttacgataca gaagatggca tgaagttcat tcttagccca ccgctgcgta
    13381 acgtacgcga gcaggacaaa ctgtggtgtg gcatcagcga tggtgcgatt gacgtggtgg
    13441 cgaccgatca ctgcaccttc tcgatggctc aacgcctgca aatttctaaa ggcgatttca
    13501 gtcgctgccc aaatggctta cccggtgtgg aaaaccgcat gcagttactg ttttccagtg
    13561 gcgtgatgac gggacgtata acaccggaac gctttgttga attaaccagc gcaatgcccg
    13621 ccaggctgtt tggcctgtgg ccgcaaaaag gattattagc gcccggttcc gatggcgacg
    13681 tggtgattat cgacccacgt cagagccaac aaattcagca tcgccatctc cacgacaacg
    13741 ccgactactc gccatgggag ggttttacct gtcagggcgc gattgtcaga accttatccc
    13801 gtggtgaaac gattttctgt gacggcacct ttacaggcaa agccgggcga ggtcgtttcc
    13861 tgcgacgcaa accgtttgtc cctcccgtgc tctaaccact gtcagggatc tatacacaat
    13921 caagaatgat gagtgaggaa aaatgagtaa gaaaattgtt ctcgccctgg gcgggaatgc
    13981 gctgggcgat gacctggccg gacaaatgaa agcggtaaaa attacttctc aggcaattgt
    14041 tgatttaatt gcccaaggac atgaagttat cgtcactcat ggtaacgggc cgcaggtggg
    14101 catgattaac caagcctttg aagctgccgc aaaaactgaa gcacactcgc caatgctgcc
    14161 gatgtctgtc tgtgttgcct taagccaggg ttatattggt tacgatctac aaaacgcgtt
    14221 aagagaagaa ctgctttctc ggggcattaa taaacctgta gcaacgctgg ttactcaggt
    14281 cgaagtcgac gctaacgatc cggcattcct caacccgacc aagccgatcg gttcgttctt
    14341 taccgagcaa gaagcagagc aactgacaaa acagggttac actctgaaag aagatgctgg
    14401 tcgcggctat cgccgtgttg ttgcctcgcc aaaaccggtt gatatcattg aaaaagaaac
    14461 ggttaaagct ctggtagatg caggccaggt ggtgatcgcc gttggcggtg gtggtatccc
    14521 tgttattcgt gaaggtaacc atctgcgtgg tgccagcgcg gttatcgata aagactgggc
    14581 cagcgcccgt ttagcagaaa tgattgatgc cgatatgctg atcattctga ctgcggtaga
    14641 aaaagtggcc attaacttcg gtaaagagaa tgaacagtgg ctcgatcgcc tgtcattaag
    14701 tgatgctgaa cgctttatcg aagaaggtca ttttgcgaaa ggttctatgc tgcctaaagt
    14761 ggaagctgcc gcctcgtttg ctcgctctcg cgcaggtcgt gaagcactga ttaccgtatt
    14821 gagcaaagcg aaagaaggga ttgaaggcaa aaccggaacg gtgatttgtc agtaatttta
    14881 aggccgggtg ggtaatacca tccggccatt ttcatttaag ccacttccag cacttctttt
    14941 gtcgctttca cgccctgatg catcaacatc attaacgcct caagtacgcc gccgccaatc
    15001 gcccgggctt tatcagaaac gctggtgaaa tcagcggttt caccacgagg atcgatatca
    15061 ccaattttaa aaccaccaac cactgccagg ccgtcgttca acaagccacg caccatcccc
    15121 gtcaacggtg ctttaatttc atgctcacca atccaggcaa tcacatcgcc ctctttcacc
    15181 agatcgccta atttcacgtt ggatcgcata atgcctgcag caggagcacg gatcacccgt
    15241 cgggtggtat gccccataat attgccagga acatcggtat tctcctgcgc acaaccagag
    15301 taaatcacct gaccgagcca gtgcccgcga tttgtttcaa ttaccgcatg acaatccttc
    15361 cctgcagtaa agcccggccc aagcgcgatt gttactggtg ccatatctgc ccgcgttccc
    15421 aaattctgtt tcgccagaat agcgtccacc acgcaaagcg gtttcagttc atcaagcaat
    15481 gaacaggcgg gatctaccat cacagggatg aatccgcgtt cggtaagttt catcgcttcc
    15541 gcagagctgg ttgccaggcg agcagtgacg ccttcgaccg tcatttcgcc atcgaacacg
    15601 gcctgggcaa acgccacggt acaacgaatc actgtcggtt tttccacttc cagcatgatc
    15661 actttaaaac ccgcatgata cagacgtagc gccacaccac tggcgatatc tcccgcaccg
    15721 cgaatcacca ccagctggtg tccggaagga tgagaaggtt tcatcatcag cccacccggc
    15781 gcgttatttt tcacccataa tatttctgcc agcacgctga tggcgatctc ctgcggcgtt
    15841 tccgcaccta tgttgtaacc aacgggcgca tgtaaacggg caatatgttc ttcagccacg
    15901 cctttctcac gcaattgacg caggaaaagc tgaaccttgc ggcgacttgc cagcaacccc
    15961 aaccatgcaa tgggctgttc aatgagtttg tcgagggctt cacgatcctg attattcgtg
    16021 gcaatcagga caaaattatc agggcgaata tccagttctt ccactgccgc accaaacgac
    16081 tcagcatgga gaagcgtggt tgatggtggg aatagttcag gattgaggct ttcgcgataa
    16141 atatcggcaa cggcaatatc aaatcctaat agcgccgcac tctgggctat cgcccggttg
    16201 acatgccccg cgccaatcaa caccagacgt ggacgcatac catggacgct gataaatact
    16261 gacattgcac ctccacaatc tgacccgaca gcatccgcac cgttacgagc catacgtcca
    16321 tggaataatc gcggcttacg ttcctgcaat gcctgaagtg actcttcaat caccttccgc
    16381 tcaaccattc cgccaccaat tgtaccgacg atagaaccat cggcgcgcac taacatttgt
    16441 gcagaatggc ggggagtcga gcctcggcta tcaacaattt gcgccatcgc aaacggacaa
    16501 ttttgctctt cgagttttgc agcctctgtg aaaatattca taccaacctc aatggatatc
    16561 ctttcagtaa cccggaatac ccgggccgct aattcacaaa tctgcgcgcg attgcaggat
    16621 gttcttgtat atcgcccagc cagattgcct ctacgtcgtg ttgttgcagc ggttgaagga
    16681 gctcgctttg tgcaatcgca ttctcacatt gagaaaaacg gttaatgaac cagactcgcc
    16741 gacaaccttg cggtacgttt ttaaacgccc cctgagggtg gcgaaccagc gcaacgagat
    16801 cgctcagttg caaggttgca tcaggtgtta acccggtaat atcagcaaac tgcgaccagc
    16861 gatggacatt ttctgtgctc actttcgcgc ccaaagtatg tcctcccatc acagcaatca
    16921 cgcagcaact gcttttgggt atgcaaggtt cgtgctcatc aggcgctttt aacggcattc
    16981 cacgcgagcc atctgcttca atgagaatta cgtcacactc tggtcgttgc accagtgcat
    17041 caatcgcttc tggcgtaaat ccctgcactt ttccctggtt cgctttccag ctgtgaaaac
    17101 aaaatgaaat gggggatgta agagacgcat gaggaagcat ggcgggatca cgacagaaaa
    17161 ccacgggcca gtgagacgtt ggcataaaca tatgtgtcgt cgtcgtgatt aacacacgcc
    17221 tgccgctagc ctggagcagt tctgccagcc aaaaaagcaa gctggttttc cccccagcac
    17281 caacaacaga aatcacagtg ggacgtttct gcgcacctaa atcaatgact aatgccgatg
    17341 ggtcaactat acttttcacg agtctttatg acctctctgg gataaattat ccccaactta
    17401 atccatcagg aagtaacgca attatcaggc gttattagcc cctataaata atggaaccac
    17461 tatgtcagcc atcgactgta taattaccgc tgccggatta tcatcaagaa tggggcaatg
    17521 gaaaatgatg ttcccctggg aacagggaac aattcttgat acaagtatca aaaatgcgtt
    17581 gcagttttgt agccgaatta ttttagtcac cggctatcgt ggtaatgaac tgcacgaacg
    17641 ttatgcgaac cagagcaata tcactattat tcacaaccca gattatgcgc agggtttact
    17701 aacatccgtc aaggccgcag tacccgcagt gcaaacagaa cattgttttc tcacccacgg
    17761 tgacatgcca accctcacca tcgatatttt tagaaaaatc tggtcgttac gaaatgatgg
    17821 cgcaatactg ccactccata atggcatccc cggccatccg attttagtgt caaaaccatg
    17881 cctgatgcag gcaatccagc gacccaatgt caccaatatg cgtcaggcac tccttatggg
    17941 agatcattat tccgtcgaaa tagaaaatgc agaaataatt ttagatattg atactccgga
    18001 cgactttatt acagcgcaaa aaaggtatac taaaatttag aaaatgtagt taaacgattg
    18061 cgttcaaata tttaatctct ccagcactct ttatcataat gataaggaat cactgatttt
    18121 gagaaaagag aaaaagtgat aaatgggctg acaataataa ttttcaggag tgattttaat
    18181 tctcatttgg cacctgtgag ttcattcaca aaaagccttt aagtttcgag ccacctcaca
    18241 tttttttata tttcccgcca aacctggcaa gagtggtgcg attgttgctc tatcccccta
    18301 aaccaccgga tttctcaaca ccggtcactc aatgatatct gtataagcta aggagagggt
    18361 tatgggggat attatgcgtc ccattccgtt tgaggaactt ttgacgcgca tatttgatga
    18421 ataccaacaa caacgctcaa tctttggtat tcccgagcaa cagttttact cacctgtaaa
    18481 aggtaaaact gttagcgtct tcggtgaaac ctgtgccact cccgtcggcc ctgccgctgg
    18541 cccgcacacg cagctcgcgc aaaatattgt cacttcctgg ctgactggcg gacgcttcat
    18601 cgaactaaaa accgtccaaa ttcttgaccg cctggagctg gaaaagccct gtattgatgc
    18661 cgaagacgaa tgctttaaca ccgaatggtc taccgaattt accttgctta aagcctggga
    18721 tgaatacctc aaagcctggt ttgccctgca ccttctcgaa gcgatgttac agccttctga
    18781 ttccggtaaa tcgttcatct ttaatatgag catcggttac aacctcgaag gtattaagca
    18841 accgccgatg caacagttca tcgacaatat gatggacgca tctgaccatc cgaaattcgc
    18901 tcaatatcgc gatacgctga ataaattact ccaggatgac gcatttttag cccgccacgg
    18961 attgcaggaa aaacgcgaaa acttgcaagc cttacccgct cgcatcccca ccagtatggt
    19021 gcagggcgtc cccctctcca ccatgcacgg ctgtcctccg catgaaatcg aagccatttg
    19081 ccgctacatg ctggaagaaa aagggctcaa cacctttgtc aaacttaacc cgaccttact
    19141 ggggtacgcg cgtgttcgtg agatcctcga tgtctgcggt ttcggttaca taggcttaaa
    19201 agaagagtca tttgatcacg acctcaagct gacgcaagca ctggaaatgc tggagcgcct
    19261 gatggtactg gcaaaagaaa aatcactcgg ctttggcgta aaactgacta acactctcgg
    19321 caccatcaac aataaaggcg cactgcctgg tgaagagatg tatatgtcag gccgtgcgct
    19381 gttcccgctc tccatcaatg ttgcagcagt tctctctcgc gcctttgacg gcaaactgcc
    19441 catttcttat tccggtggtg ccagtcagct gactatccgc gatatttttg atacaggtat
    19501 tcgccctatt actatggcaa ccgacctgct gaaacctggc ggctatctgc gcttaagtgc
    19561 ctgcatgcgc gagctggaag gctccgacgc ctggggactt gaccatgttg acgtcgaacg
    19621 actgaacaga ctggcagcag acgcgttaac catggaatac acccagaaac actggaagcc
    19681 agaagagcgt attgaagtgg cagaagacct gccgctgacc gattgctacg ttgccccctg
    19741 tgttactgcc tgcgctatca agcaagatat tccggaatac atccgtctgc ttggcgaaca
    19801 ccgctatgcc gacgcgctgg aactcatcta ccaacgcaac gccctacctg ccattaccgg
    19861 tcatatttgc gatcaccagt gccaatacaa ctgtacccgc ctggattacg acagtgcgct
    19921 gaatatccgc gaactgaaaa aagtcgcgct ggaaaaaggt tgggatgaat ataagcaacg
    19981 ctggcacaaa cccgccggtt ctggttcacg ccatccggtt gccgtgattg gtgcaggtcc
    20041 ggcgggtctg gcagcaggtt acttccttgc cagagcgggc catccggtta cgctgtttga
    20101 acgcgaagcc aatgctggcg gcgtggtgaa aaatatcatt cctcagtt
//
LOCUS       DADMKF010000069        19741 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.070, whole genome shotgun sequence.
ACCESSION   DADMKF010000069 DADMKF010000000
VERSION     DADMKF010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19741
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.070"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            <1..511
                     /gene="csgD"
                     /locus_tag="JNC24_002272"
     CDS             <1..511
                     /gene="csgD"
                     /locus_tag="JNC24_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator CsgD"
                     /protein_id="HAY9159006.1"
                     /translation="DDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPE
                     DYPYRDIENWPHINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYN
                     STESALLTHREKEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAV
                     SWANDNLRR"
     gene            516..905
                     /gene="csgE"
                     /locus_tag="JNC24_002273"
     CDS             516..905
                     /gene="csgE"
                     /locus_tag="JNC24_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HAY9159007.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALLSTGDLAHDEF"
     gene            930..1346
                     /gene="csgF"
                     /locus_tag="JNC24_002274"
     CDS             930..1346
                     /gene="csgF"
                     /locus_tag="JNC24_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HAY9159008.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            1373..2206
                     /gene="csgG"
                     /locus_tag="JNC24_002275"
     CDS             1373..2206
                     /gene="csgG"
                     /locus_tag="JNC24_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
                     /protein_id="HAY9159009.1"
                     /translation="MQRLFLLVAVMLLSGCLTAPPKEAARPTLMPRAQSYKDLTHLPA
                     PTGKIFVSVYNIQDETGQFKPYPASNFSTAVPQSATAMLVTALKDSRWFIPLERQSLQ
                     NLLNERKIIRAAQENGTVAINNRIPLQSLTAANIMVEGSIIGYESNVKSGGVGARYFG
                     IGADTQYQLDQIAVNLRVVNVSTGEILSSVNTSKTILSYEVQAGVFRFIDYQRLLEGE
                     VGYTSNEPVMLCLMSAIETGVIFLINDGIDRGLWDLQNKAERQNDILVKYRHMSVPPE
                     S"
     gene            complement(2271..2762)
                     /locus_tag="JNC24_002276"
     CDS             complement(2271..2762)
                     /locus_tag="JNC24_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HAY9159010.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(2864..3418)
                     /locus_tag="JNC24_002277"
     CDS             complement(2864..3418)
                     /locus_tag="JNC24_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HAY9159011.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMAQVSADYNALFIGDECAVPPYRSAWVEDVTEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            complement(3442..4179)
                     /locus_tag="JNC24_002278"
     CDS             complement(3442..4179)
                     /locus_tag="JNC24_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HAY9159012.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMIATIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            complement(4234..5172)
                     /gene="ghrA"
                     /locus_tag="JNC24_002279"
     CDS             complement(4234..5172)
                     /gene="ghrA"
                     /locus_tag="JNC24_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
                     /protein_id="HAY9159013.1"
                     /translation="MDIIFYHPTFDTQWWIEALRKAIPQARVRAWKSGDNDSADYALV
                     WHPPVEMLAGRDLKAVFALGAGVDSILSKLQAHPEMLNPSVPLFRLEDTGMGEQMQEY
                     AVSQVLHWFRRFDDYRIQQNSSHWQPLPEYHREDFTIGILGAGVLGSKVAQSLQTWRF
                     PLRCWSRTRKSWPGVQSFAGREELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPD
                     GAYLLNLARGVHVVEDDLLAALDSGKVKGAMLDVFNREPLPPESPLWQHPRVTITPHV
                     AAITRPAEAVEYISRTIAQLEKGERVCGQVDRARGY"
     gene            5406..5493
                     /locus_tag="JNC24_002280"
     tRNA            5406..5493
                     /locus_tag="JNC24_002280"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5440..5442,aa:Ser,seq:gga)
     gene            5538..5674
                     /locus_tag="JNC24_002281"
     ncRNA           5538..5674
                     /ncRNA_class="other"
                     /locus_tag="JNC24_002281"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(5765..6220)
                     /locus_tag="JNC24_002282"
     CDS             complement(5765..6220)
                     /locus_tag="JNC24_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001462353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HAY9159014.1"
                     /translation="MVAEGILLERKEWLITRCEKMYFDNLEKWFGHYCHIHCQVSLGR
                     LINFPEQPEFSEEERKRFFAIYNGMAMDYVLVSKKNNKIVCVIELDDPSHERADRIIR
                     DRWLDTIMTLARVPFVRASVTKLEEIPPVWETRDKIRPLVAATKSEPVH"
     gene            complement(7032..8096)
                     /gene="phoH"
                     /locus_tag="JNC24_002283"
     CDS             complement(7032..8096)
                     /gene="phoH"
                     /locus_tag="JNC24_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HAY9159015.1"
                     /translation="MVTSCTGHVLDNQRATTRGVFSSGSHLVTLHFQPHPFFSCVTDA
                     VNGARSRFSAFYPKANYGLQGSQPSDVRAHNRAANGACDEYKQLKVLSMGRQKAVIKA
                     RREAKRVLRRDSRSHKQREEESVTSLVQMGGVEAIGMARDSRDTSPILARNEAQLHYL
                     KAIESKQLIFATGEAGGGKTWISAAKAAEALIHKDVDRIIVTRPVLQADEDLGFLPGD
                     IAEKFAPYFRPVYDVLVRRLGASFMQYCLRPEIGKVEIAPFAYMRGRTFENAVVILDE
                     AQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLPRGVCSGLSDALERFEEDEMVGIVR
                     FGKEDCVRSALCQRTLHAYS"
     gene            complement(8441..9712)
                     /gene="efeB"
                     /locus_tag="JNC24_002284"
     CDS             complement(8441..9712)
                     /gene="efeB"
                     /locus_tag="JNC24_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HAY9159016.1"
                     /translation="MQYKDENGVNEPSRRRLLKGIGALALAGSCPVAHAQKTQSAPGT
                     LSPDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLT
                     QGGAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKM
                     TRFPNDSLDAALCHGDVLLQICANTQDTVIHALRDIIKHTPDLLSVRWKREGFISDHV
                     SRSKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQ
                     FRVEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLAN
                     PRTAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALE
                     EYVKPIGGGYFFSLPGVKDANDYLGSALLRV"
     gene            complement(9718..10845)
                     /locus_tag="JNC24_002285"
     CDS             complement(9718..10845)
                     /locus_tag="JNC24_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HAY9159017.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADVPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYAEQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(10903..11733)
                     /locus_tag="JNC24_002286"
     CDS             complement(10903..11733)
                     /locus_tag="JNC24_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HAY9159018.1"
                     /translation="MFVPFLIMLREGLEAALIVSLIASYLKRTQRGRWIGVMWIGVLL
                     AAALCLGLGIFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNVKVQLE
                     QAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAMLGLAT
                     AVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQEIAFD
                     MSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGATASRSA
                     "
     gene            complement(12399..13907)
                     /gene="putP"
                     /locus_tag="JNC24_002287"
     CDS             complement(12399..13907)
                     /gene="putP"
                     /locus_tag="JNC24_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HAY9159019.1"
                     /translation="MAISTPMLVTFCVYIFGMILIGFIAWRSTKNFDDYILGGRSLGP
                     FVTALSAGASDMSGWLLMGLPGAVFLSGISESWIAIGLTLGAWINWKLVAGRLRVHTE
                     YNNNALTLPDYFTGRFEDKSRILRIISALVILLFFTIYCASGIVAGARLFESTFGMSY
                     ETALLAGAASTILYTFIGGFLAVSWTDTVQASLMIFALILTPVIVIISVGGFGDSLEV
                     IKQKSIENVDMLKGLNFVAIISLMGWGLGYFGQPHILARFMAADSHHSIVHARRISMT
                     WMILCLAGAVAVGFFGIAYFNEHPAVAGAVNQNAERVFIELAQILFNPWIAGILLSAI
                     LAAVMSTLSCQLLVCSSAITEDLYKAFLRKQASQKELVWVGRVMVLVVALVAIALAAN
                     PENRVLGLVSYAWAGFGAAFGPVVLFSVMWSRMTRNGALAGMIIGALTVIVWKQFGWL
                     GLYEIIPGFIFGSIGIVVFSLLGKAPSAAMQKRFAEADAHYHSAPPSRLQEN"
     gene            14329..18291
                     /gene="putA"
                     /locus_tag="JNC24_002288"
     CDS             14329..18291
                     /gene="putA"
                     /locus_tag="JNC24_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001462364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
                     /protein_id="HAY9159020.1"
                     /translation="MGTTTMGVKLDDATRERIKSAATRIDRTPHWLIKQAIFSYLEQL
                     ENSDTLPELPALLSGAANESDEAPTPAEEPHQPFLDFAEQILPQSVSRAAITAAYRRP
                     ETEAVSMLLEQARLPQPVAEQAHKLAYQLADKLRNQKNASGRAGMVQGLLQEFSLSSQ
                     EGVALMCLAEALLRIPDKATRDALIRDKISNGNWQSHIGRSPSLFVNAATWGLLFTGK
                     LVSTHNEASLSRSLNRIIGKSGEPLIRKGVDMAMRLMGEQFVTGETIAEALANARKLE
                     EKGFRYSYDMLGEAALTAADAQAYMVSYQQAIHAIGKASNGRGIYEGPGISIKLSALH
                     PRYSRAQYDRVMEELYPRLKSLTLLARQYDIGINIDAEEADRLEISLDLLENLCFEPE
                     LAGWNGIGFVIQAYQKRCPLVIDYLVDLATRSRRRLMIRLVKGAYWDSEIKRAQMDGL
                     EGYPVYTRKVYTDVSYLACAKKLLAVPNLIYPQFATHNAHTLAAIYQLAGQNYYPGQY
                     EFQCLHGMGEPLYEQVTGKVADGKLNRPCRIYAPVGTHETLLAYLVRRLLENGANTSF
                     VNRIADTSLPLDELVADPVTAVEKLAQQEGQTGLPHPKIPLPRDLYGHGRDNSAGLDL
                     ANEHRLASLSSALLNSALQKWQALPMLEQPVAAGEMSPVINPAEPKDIVGFVREATPR
                     EVEQALESAVNNAPIWFATPPAERAAILHRAAVLMESQMQQLIGILVREAGKTFSNAI
                     AEVREAVDFLHYYAGQVRDDFANETHRPLGPVVCISPWNFPLAIFTGQIAAALAAGNS
                     VLAKPAEQTPLIAAQGIAILLEAGVPPGVVQLLPGQGETVGAQLTGDDRVRGVMFTGS
                     TEVATLLQRNIASRLDAQGRPIPLIAETGGMNAMIVDSSALTEQVVVDVLASAFDSAG
                     QRCSALRVLCLQDEIADHTLKMLRGAMAECRMGNPGRLTTDIGPVIDSEAKANIERHI
                     QTMRSKGRPVFQAVRENSEDAREWQSGTFVAPTLIELDDFAELHKEVFGPVLHVVRYN
                     RNQLPELIEQINASGYGLTLGVHTRIDETIAQVTGSAHVGNLYVNRNMVGAVVGVQPF
                     GGEGLSGTGPKAGGPLYLYRLLANRPESALAVTLARQDAEYPVDAQLKAALTQPLNAL
                     REWAANRPELQALCTQYGELAQAGTQRLLPGPTGERNTWTLLPRERVLCIADDEQDAL
                     TQLAAVLAVGSQVLWPDDALHRQLVKALPSAVSERIQLAKAENITAQPFDAVIFHGDS
                     DQLRALCEAVAARDGAIVSVQGFARGESNILLERLYIERSLSVNTAAAGGNASLMTIG
                     "
     gene            complement(18331..18969)
                     /gene="rutR"
                     /locus_tag="JNC24_002289"
     CDS             complement(18331..18969)
                     /gene="rutR"
                     /locus_tag="JNC24_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HAY9159021.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEIFFNQTVENVQRIIIEGIRPR"
     gene            19257..>19741
                     /locus_tag="JNC24_002290"
     CDS             19257..>19741
                     /locus_tag="JNC24_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HAY9159022.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPVYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     D"
BASE COUNT         5012 a         5255 c         4809 g         4665 t
ORIGIN      
        1 ggacgatata tcttcaggct ctattattct tctggatatg atggaagcgg ataaaaagct
       61 tatccattat tggcaggata ctttgagcag gaaaaacaac aatatcaaaa tattgttgct
      121 aaatacgcct gaagattacc cgtaccgcga cattgaaaac tggcctcata tcaacggcgt
      181 tttttatgcc atggaggatc aagaacgtgt tgtcaatggg ttgcaaggcg tcctgcgcgg
      241 cgaatgctac tttacgcaaa agcttgccag ctacttgatt acgcattcag gtaactatcg
      301 ttataacagc acggaatcag ccctccttac tcatcgggaa aaagagatcc tgaataaact
      361 gcgtatcggc gcgtctaata acgagatcgc tcgttcgttg ttcatcagcg aaaatacggt
      421 taaaacgcat ctttataatc ttttcaagaa gatagccgta aaaaaccgga cacaagcggt
      481 ttcctgggca aacgataacc tcaggcgata aagccatgaa acgttattta cgctggattg
      541 tggcggcaga atttctgttc gccgcaggga atcttcacgc cgttgaggta gaagtcccgg
      601 gattgctaac tgaccatact gtttcatcta ttggccatga tttttaccga gcctttagtg
      661 ataaatggga aagtgactat acgggtaact taacgattaa tgaaaggccc agtgcacgat
      721 ggggaagctg gatcactata acggtcaatc aggacgttat tttccagact tttttatttc
      781 cgttgaaaag agacttcgag aaaactgtcg tctttgcact gattcaaact gaagaagcac
      841 taaatcgtcg ccagataaat caggcgttat taagtacggg cgatttggcg catgatgaat
      901 tctaaataaa aaattgttcg gaggctgcaa tgcgtgtcaa acatgcagta gttctactca
      961 tgcttatttc gccattaagt tgggctggaa ccatgacttt ccagttccgt aatccaaact
     1021 ttggtggtaa cccaaataat ggcgcttttt tattaaatag cgctcaggcc caaaactctt
     1081 ataaagatcc gagctataac gatgactttg gtattgaaac accctcagcg ttagataact
     1141 ttactcaggc catccagtca caaattttag gtgggctact gtcgaatatt aataccggta
     1201 aaccgggccg catggtgacc aacgattata ttgttgatat tgctaaccgc gatggtcaat
     1261 tgcagttgaa cgtgacagat cgtaaaaccg gacaaacctc gaccatccag gtttcgggtt
     1321 tacaaaataa ctcaaccgat ttttaagccc cagcttcata aggaaaataa tcatgcagcg
     1381 cttatttctt ttggttgccg tcatgttact gagcggatgc ttaaccgccc cgcctaaaga
     1441 agccgccaga ccgacattaa tgcctcgagc tcagagctac aaagatttga cccatctgcc
     1501 agcgccgacg ggtaagatct ttgtttcggt atacaacatt caggacgaaa ccgggcaatt
     1561 taaaccctac ccggcaagta acttctccac tgctgttccg caaagcgcca cggcaatgct
     1621 ggtcacggca ttgaaagatt ctcgctggtt tataccgctg gagcgtcaga gtttacaaaa
     1681 cctgcttaac gagcgcaaga ttattcgtgc ggcacaagaa aacggcacag ttgccattaa
     1741 taaccgaatc ccgctgcaat ctttaacggc ggcaaatatc atggttgaag gttcgattat
     1801 cggttatgaa agcaacgtta aatcgggcgg tgttggggca agatattttg ggatcggtgc
     1861 agacacgcaa taccagctcg atcagattgc cgtgaacctg cgtgtcgtca atgtgagtac
     1921 cggtgagatc ctctcctcgg tgaacaccag taagacgata ctttcctatg aagttcaggc
     1981 cggggttttc cgctttattg actaccagcg cttgctggaa ggggaagtgg gttacacctc
     2041 aaacgaaccc gttatgctgt gtctgatgtc ggctatcgaa acaggggtca ttttcctgat
     2101 taatgatggt atcgaccgtg gtctgtggga tttgcagaac aaagcagaac ggcagaatga
     2161 cattctggtt aaataccgcc atatgtcggt tccaccagaa tcctgacaga taaaaatgcc
     2221 ggaattaata atccggcatt ttactgttta agcgaaagcg tgagtgcccc tcacgcttta
     2281 tttttgattt cctgctcacg gtgagccgtc tttgcactac gcgccgccag ccacaggccg
     2341 ctgtttttca ttgcgtaacc aaataccagc ccaagcgcca gcgaaggtaa caccagtttc
     2401 cagtcaccct gcccggcaaa cgtcgcgcat gcgcctataa acgtccccgg aacaaatgaa
     2461 agcagcagct gtttggcctg aatacacatc agaaacgcca caataccggt tatgacataa
     2521 ccgagaattt ccagatgtgg tgccagtgca ctaccgtaaa taatgaccat cgcccacacc
     2581 acgccactaa gcagggttgc agcggagatc gccagccctt tcagcccacc ttgcgggcag
     2641 gcaaagtagg ccgtacagcc caagaagccc gcccagctta gtaagcccag ggaaacagcc
     2701 acccatcccc agataccgga gagaatgcct gttgtgattg caatagagag aagtatgttc
     2761 atgccgcgca tgatagcaga aaacgcggcc atgaatgaga tcgagcacac attttatgca
     2821 acaacaaatt atctgttgca gttatgagtg acgatgatca catttactct tcagaatctt
     2881 cttccagctc gtcccacatt gcactaatgg catcacgggt tagcggtgcc atggtgcgcc
     2941 agaaaggcgt ggttgcatgg gcctccactt tgccgaggaa cgcaccacac cagggtaaca
     3001 gatactcact gaacagtgtt tccagtgctt cgctctcatc ttccgttgac tgatcttcca
     3061 gccaggaagc cgcaagcagc aatgtgccga tgtgatcggc tggcgtatcc gccagtggca
     3121 tccctcgttc ggaaagaaaa gcgcgcactt ccgcttccgt tacgtcctca acccatgcgc
     3181 tacgatatgg cggcactgca cattcatcgc cgataaacaa cgcattgtaa tcggcagaga
     3241 cttgcgccat atcacagctt ttctgtaaac gcgtcagtaa ctcatcctgc tccagtggcc
     3301 agttcgcagc cagtttcccc tcacgaatca gggtaaacag cggcaccagt aaaggatctt
     3361 gcggttggcg gtaatagagc gaacccagca cacgacagag gatagaaaac tcgttcatta
     3421 atatatttcc attactagta attaaaggtc tgcaaattcc gcaatcggtg ccataccgcg
     3481 agattcaagg aagttcagta agcggcgcgg agaaacattc aaaatgcgct ctggcggaaa
     3541 atctaccgcg tcgaggattt taagacactc ttcaaattcc cccatggtaa acgcggtgtg
     3601 agaatccgag cctaatgcca cccaacctcc agcatcacgt accgctgcgg ctaccgcacg
     3661 acagttgtct tcactgccct tacgtgagtg taaaaatgaa gaattattga tttccagcgc
     3721 cacctgatgt ttcgcggctg cttcagcaac ggctttcaca tctatttcat atttgggatt
     3781 tccgggatgg cttattatat gcacattgcc gctggcgata gtggcgatca tcgcttgtgt
     3841 attggtcgct ttgtcatgtg gtgcaaaaac cggctcatga aaaccggcaa taatcagatc
     3901 cagcgagtca aacattttac cgctgcaatc aatttcacca tcaacatttt taatgttagc
     3961 ttcgatgccg cgcaggatcc ctaccccatc aaccactcgc ggccagatac gcatgttaat
     4021 gaagtgccag tgatgcggag catcttccat atccgggcca tgatcggtga tcgcaaaaag
     4081 tttaatgccc ttttgtttgg cctgggcaat gtaatcactc aatgtgctat atgcatgtgt
     4141 gctggcaacg gtatgcatat gaaggtcgac gggatacata actctctcct gtattcaatt
     4201 cccgccaagg atagcaggaa tcctgatgct ttattagtag ccgcgtgcgc ggtcgacttg
     4261 cccgcagacc ctctcccctt tttcgagctg ggcaatggtg cgagaaatgt actccacagc
     4321 ttcagcggga cgggtaatcg cggcgacatg tggtgttatc gtcacgcgtg gatgttgcca
     4381 gagcggactt tcaggcggta agggttcacg attaaaaaca tccaacattg cgcctttaac
     4441 tttgccgcta tccagcgccg cgagcaggtc atcttccaca acatgaacac cacgcgccag
     4501 gttgaggaga tacgcgccat ccggtaattt ttcgagtaat tgttgattaa taatgccgac
     4561 ggtttcaggg gtattcggta acaaattaat caatacccga cattggctca gaaatgcaga
     4621 cagttcttcc cgtccggcaa agctttgcac gccaggccac gatttacggg ttcgactcca
     4681 gcaacgcagc ggaaagcgcc aggtttgcag actctgagca actttactgc ccagtacgcc
     4741 tgcgcccaaa atgccgatgg taaaatcttc ccgatgatat tcaggcagcg gttgccaatg
     4801 cgaactattt tgctggatgc gataatcgtc aaaacgtcga aaccaatgca gcacctgact
     4861 gacagcatat tcctgcattt gctcgcccat accggtatct tccaggcgaa aaagtggaac
     4921 agaagggttc agcatttcag ggtgtgcctg tagcttgctc aaaatagaat caacaccggc
     4981 cccgagtgcg aacaccgctt taagatcgcg ccctgccagc atttcaacag gaggatgcca
     5041 gactaaagca taatcagcag agtcattatc tccgcttttc catgctctga ctcttgcctg
     5101 aggaatagct ttgcgcagtg cctcaatcca ccattgggta tcgaacgttg ggtgataaaa
     5161 gatgatatcc atactgactc ccgaaaagcg ttgtgcgaaa tttattcgca cttatcgtta
     5221 tgatctacaa aggccaccag cataacaaat ccgtggtcgg tggcaaaaaa agcagatttc
     5281 gcttattaaa accacacatt gattgaagtt tgaataaacg cgcgattttt tcaaaaagtt
     5341 tgttgacctc aggtcatgat ttccctaaat tagcgcccgt tccagcaaga caggaacgac
     5401 aatttggtga ggtgtccgag tggctgaagg agcacgcctg gaaagtgtgt atacggcaac
     5461 gtatcggggg ttcgaatccc cccctcaccg ccatatttaa agaagagctc gtacgaaagt
     5521 acgggctttt ttttcgtata ttgcacacac cggggggatg agaagccccg accggggttc
     5581 gacaactggc gacagccagt tggacagacc gtgaacgcag tgagcgggct gcccgcaggg
     5641 cgagcgaagc gagtcaatcc ccccctcacc gccatcttta aagaagagct cgtacgaagg
     5701 tacgggcttt ttttcgtata ttgcacacac cggggggatg agaagccccg accgggatac
     5761 ttagttaatg caccggttca ctctttgtcg ccgcgactaa tgggcgaatt ttgtcacgag
     5821 tctcccagac cggaggaatt tcctccaatt tagtgacaga agctcgtaca aaaggcaccc
     5881 gtgcaagagt cataattgta tccaaccaac ggtcacgaat aatgcgatcc gccctttcat
     5941 gactcgggtc atccagctca ataacacaga ctattttatt gttcttcttt gaaaccagaa
     6001 cataatccat tgccatgcca ttataaattg caaagaaacg ctttctttct tcttcgctaa
     6061 actcaggttg ttctggaaag ttgatcaagc gccccagaga gacctggcaa tggatatgac
     6121 aataatgtcc aaaccatttt tccagattat cgaaatacat tttttcacaa cgcgtgatta
     6181 accactcttt tctttccaat aaaatacctt cggcaaccat tccctgaaat aactcatctc
     6241 gctcttgcca acgagagtca cgataggttt tctgcggtat atagctcctt gtcgacgcct
     6301 tagcggcagg ggaagttgca gatgaaggga cgggttgcga tttagcgatg attaccgatg
     6361 aggcaacttt tcggagtgtc aattttctgg caacagcaaa accggcgata aatgcaattg
     6421 tcaaacaagt tacaaaagcc ataaagctca taattaattt tcacctatcc atgttgatta
     6481 tatttcatta ccaatgtaac tcgctgcagc gtgttacatg ccaacaatgc aacgatcaat
     6541 atatagttag tatcatgttt tgattattac aaccatccct atttagccat tgcggcttgc
     6601 cttccacaaa aaaataacaa cacacaatat tagcaaagaa ttaattaaga ttaaccttac
     6661 atttataata ttatccctaa agaataaatc agaggaatat attcggtgtt aaacagtcag
     6721 tcaaagtata gcccattgtt gattaaacct cataagcaaa attggcacca gatctcctgt
     6781 acaggttgaa tggccgaaaa aagataggca gatgctcatt tttttggctt ctggaagaaa
     6841 aactctccgc gctggcagta ttacactggt ctgatgctat gacgaaaaat gcctggtaag
     6901 agtatctaaa ccagactcca gttcagctta tcaacacgct caaagtcgcc cccctgaacg
     6961 ctaacctgaa gtgaaaatct gttgaaataa aacaaagccc ggttcgcccg ggctctgcac
     7021 cgataacaca cttaactgta ggcatgcagc gtacgttggc aaagtgccga acgtacgcag
     7081 tcctctttac cgaaccggac gatcccaacc atttcatctt cttcgaaacg ttccagcgcg
     7141 tcacttaatc cggagcacac gccgcgaggc aaatcgcatt gcgtgatatc accgttgacg
     7201 ataaccgtca cgttctcccc gaggcgggtt aaaaacattt tcatttgcgc ggcagtcaca
     7261 ttctgcgcct cgtcaagaat gacgactgca ttttcaaagg tacgtccacg catataggcg
     7321 aacggcgcaa tttccacctt ccctatttcc ggtcgcaggc agtactgcat aaaggaagcc
     7381 cctaagcgcc ggaccagcac gtcgtagacc gggcgaaaat agggagcaaa cttttctgcg
     7441 atatctccag gtaagaagcc aagatcttca tcggcttgca gaactggacg ggtgacgata
     7501 atcctgtcga catccttatg tatcagggcc tctgccgctt ttgctgcgct gatccaggtt
     7561 tttccgcccc cggcttcgcc cgtggcgaat atcagctgct tactctcaat agccttcaga
     7621 tagtgcaatt gcgcttcatt tcgcgcgagg atgggcgaag tatcgcgact gtcgcgggcc
     7681 ataccaatgg cttctacgcc gcccatctgc acaagcgagg tgaccgattc ttcttcacgc
     7741 tgcttatggc tgcgcgaatc ccgtctcagc acacgttttg cctcgcgacg agctttgatc
     7801 actgcttttt gtcttcccat agagagcacc ttgagttgtt tgtattcatc acacgcgccg
     7861 ttggcagcgc gattatgcgc acgaacatca gagggttggc ttccttgtaa gccatagttt
     7921 gcttttggat aaaatgccga aaaacggcta cgcgcaccgt ttacggcgtc ggtaacacat
     7981 gaaaagaaag gatgaggttg aaaatgcaaa gtgacgagat gactaccgga ggagaaaact
     8041 ccgcgagtgg tggcgcgttg attatctaaa acatgtccag tacaggacgt taccatccgc
     8101 gatctccata gtgactgact atcactgccg ggaacttccg ctgctactta ataagtacaa
     8161 cagatctcgc atttattgca acaatatatt tacttatatt taactataaa acactatttc
     8221 agtgacatta gtttctactg gaaagatgac agagtgatga cagtgatgaa aaaagctgtg
     8281 tgatttcagc aggatttgca tcaggcatca gtgcacgatc gccggatgcg gcgtgaacgc
     8341 cttatccagc ctaccaaacc cgcactgatt gtaggcatga taagacgcgc aagcgtcgca
     8401 tcaggcatct gcgcacaaat gccttatccg cctaaaaaca ttaaacccgc aataacgcgc
     8461 ttccgagata atcgttcgcg tccttcactc ccggcagcga aaaaaaataa ccgccgccga
     8521 taggtttaac gtattcctcc agcgcttcgc cattgagcct tttttgtact gtcaggaagc
     8581 ctttttccag atcgtgttgg tagcagacaa acagcaaccc catatccagt tgtccggagt
     8641 tggtgacccc caacgaataa ctgtagccgc gacgcagcat caggctggac tcactctccg
     8701 ccgtgcgggg attcgccagc cggatatggc tgtccagcgc aatcaccttc ccttccgggt
     8761 cgctggcgta atcaggcaca tcatgctcat gctgcattcc cagcggcgca ccggtttgct
     8821 tatcacggcc aaaaatcgtc tgctgttctt tcagcggcgt tctgtcccaa aattccactc
     8881 gaaactgaat caagcgtact gcctgatagc tgccaccgat tgtccacgca ggctcctgct
     8941 gatctgccgt tacccacacc actttttgca tcaacttatc attctggcta tcgggattgg
     9001 cagtgccgtc tttgaaaccc agcaaattaa tcggcgtctc tttgccttta ctacgcgaca
     9061 cgtgatcgga aataaaccct tcccgcttcc agcgcacact gagcaaatcc ggcgtgtgtt
     9121 tgatgatatc gcgcagcgca tggataaccg tgtcctgggt gttggcgcaa atctgtagca
     9181 acacatcacc atgacataac gccgcatcca gcgagtcgtt ggggaaacgc gtcatcttct
     9241 gcagcttttt tggcatctgt ggcgcaaggc caaagcgctc atcaaacaat gagtgaccca
     9301 ccgataacgt gatggtgaga ttatcgggcg caatgtagcc gccaagaatg ccggaatcga
     9361 gtggtggcag gcgcggattt ggcgtttctg gtgctgctcc gccctgagtc agaaaagcaa
     9421 aacgctgagt caacaagcga aacaaccgct caagatcggc tttatcgctg gcaagcacat
     9481 caaacgccac cagcatcatt gcggcctgtt gtggcgtcag gatccctgcc tgatgctcac
     9541 cataaaacgg ctgtttctca ttgcgtgcat ccggtgaaag cgtgcccggt gcactttgcg
     9601 ttttttgtgc atgagcgacc ggacaacttc ccgccagcgc cagcgcacca atccctttca
     9661 gtaaacgtcg gcgtgacggt tcattcacgc cgttttcatc tttatactgc ataacgctta
     9721 atccagtccc agcacaccgc gaagttgcgc cagatcttcc gccagcgcag taatcggtcc
     9781 tttcagtgca ttccggtcgg catcggtcaa tttgtcgtag gtttcaaaac cgtctttagt
     9841 acggtatttc gccagaatgg tatcgacctt tttaaagttg gcatcgactt ttgccagcag
     9901 ttccgggttg gctttttgca gttgtggacg cagcaaatcg acaattttct gcgagccttc
     9961 aacgttagcc tggaaatccc acagatcggt gtggctgtag cgatcttctt caccgctaat
    10021 tttgctggct gccacttcct caatcagtcc ggctgcgccg ccgaccactt ttgaaggtgg
    10081 gaaagccagt tcactgatgc gtttttgcaa atcgaccaca tctgtataaa gctgctcagc
    10141 gtactgatcc atccctttgg tggtgttgtc gccaaacaat gctttttcca gacggtggaa
    10201 accagtgaat tttgggtcgg cggctttttg ctcgtaatca tcttcacggg cgtcaatgct
    10261 gccatccaga tcggagaaca gttcagcaat cggttcaatg cgctcatagt gctggcgcgt
    10321 cggcgcatac agtgctttcg ctttttcgat atcgcctgct ttaatcgcgt cggtaaaggc
    10381 tttggtgtcg gtcaccagct gcgtggtttc cgccatgaca tacgctttat atgcagtaat
    10441 tgcaccacca agacttaaca gcgcatcact ttgcgccgca tccgccgttg cctcaccttt
    10501 gacgatcaac ttccctttcg ggttagtcag cagaccgcag gtcatatcgt attcgccagg
    10561 ctgtaaattc gccgtcattt tctggctaaa accaggggcg atattttccc gctcttccac
    10621 caccatcacg cctttgagga tctcccactc cagcgccttc tggctatggt tctgaataat
    10681 gaactgtgtt ttcccggcgt taaccgtaat ggtcatcggt tcgcactgct tatccgtcac
    10741 ggtcactttg acctgcggca catcagcggc gttagccata aaagcagaag aaaacagcgc
    10801 agccacgctc aactgcaatg cgttacggcg gaagttaatg gtcatgacga gactatccct
    10861 ttaaagtatg ttgtaactaa gcaagagttt gcgtcgtatt tgttacgcgg agcgagacgc
    10921 tgtcgcccct gcgcgtggtg gcagagcaaa tgccaccagc gccgggatga gataaataaa
    10981 ccagacggcg acttcgctga cgctcggtgc ttcctgatag ccaaaaatcc cttccatcag
    11041 cgtgccaaac agcgagtgag ttgagagcac tgcgctcata tcgaaggcga tttcctgaaa
    11101 gtggttccac aatccggctt catgaaatgc gcgaatggca ccagcagcca gccctgcggc
    11161 gacgaagaga ataaacaggc tggtccattt aaaaaatgca ccaagattga ggcgaatacc
    11221 gccccagtag agcaggaagc ctagcaccac ggcagtagca agaccgagca ttgcacccag
    11281 cggcggccag atcccgacat cttgttgaaa tgccgccagc aggaaaaaga ccgactccag
    11341 cccttccctt gcaacggcaa aaaagaccat catcaccagc gcccagccat gatgatttcc
    11401 acgctgcaat gcgctatcga ctgcctgctc cagttgtact ttgacgttgc gcgacacttt
    11461 gcgcatccag aaaaccatcc aggtaaggat caccacggcg atcaccgcca cgataccttc
    11521 aaacagttcc tgttcttttt gcggaaattc gccggtggtt tcgttaatga agatacccaa
    11581 gcccaggcac aacgcagcgg caagcaacac gccaatccac atcacgccaa tccatcggcc
    11641 tcgctgggta cgcttaagat agctggcaat caaactgaca atcagcgcgg cttcaagtcc
    11701 ttcgcgcaac ataatgagaa acggaacaaa catgccaccc acccttaaac gtttttctga
    11761 gtcaatccat gcaaagaaaa gtaaatcgca gtgatagcga ttatcattac ggcaaagaaa
    11821 aatacaagcg gaatgtttat aaaatgatgt tggaatgtaa acaaagagag gtaataccag
    11881 gggatataaa cgattattcc aggtacaaag atgggattgt ttacaacgag ccatgtgttc
    11941 ttgccagatg ccgcgtaaac gccttatcca gccgacaaaa ctgtccagac tcaatgcatc
    12001 acagcttcca tgtaaaactg ataagcgggt gtatcaagca atttcgtcca tccgcgatta
    12061 tcaaatctaa ctttttgccc ggaacataat aacagcgcgg gtgttggtgg cgtcaaaatc
    12121 gctgaaacgt tccccgaagg ccggggcagc ccacatggat gtgggctgag ggcgcgtctt
    12181 acaggacgtt acctcgcgcc cgacccggta gccgaagggg ataagctgaa ggtaccgcgc
    12241 agcggcgatt ttgttcgcca gagcccgggg gggcaggggg cggcggcgac tggccgcccc
    12301 ctgcgcgctc cctgcaccat tcaatttata tggcgaggca cattaaggga gcgcaacccg
    12361 ctgctgaagc caaacaaaac cgccgcaggc taagtccctt agttttcctg caaccgtgac
    12421 ggcggagccg aatgatagtg cgcatcggcc tcggcaaagc gtttttgcat cgccgctgac
    12481 ggcgctttac ccagcaaact aaacactaca atcccgatac tgccgaagat gaagcccgga
    12541 ataatttcgt acagtcccag ccagccgaac tgcttccaga cgataactgt cagcgcaccg
    12601 atgatcatcc ccgccagcgc accgttgcgc gtcatgcgtg accacatcac cgagaacagc
    12661 accaccggac caaacgccgc gccaaagcct gcccacgcgt agctcactaa gcccagcacg
    12721 cggttttccg ggtttgccgc cagcgcaatc gccaccagcg ccaccaccag caccatcaca
    12781 cgtcctaccc acaccagctc tttctggctg gcctgtttac gcagaaacgc tttgtataaa
    12841 tcttcggtaa tcgcactgga gcacaccagc agctggcaac ttaaggttga cattaccgcc
    12901 gccagaatcg ccgacagcag aatcccggca atccacgggt taaacagaat ttgcgccagt
    12961 tcgataaaca cgcgttcagc gttctggttt accgcacctg ctaccgccgg atgctcgtta
    13021 aagtaagcga taccaaagaa gccaacagcc accgcccctg ccaggcaaag gatcatccag
    13081 gtcatactga tacgacgcgc atggacaatg ctgtggtgag aatctgccgc cataaaacgc
    13141 gccaggatgt gcggctgccc gaagtaaccc agcccccaac ccatcaatga gataatggca
    13201 acaaagttca gccctttgag catatccacg ttttcgatgc tcttttgttt gatcacttcc
    13261 agcgagtcac caaagccacc gacactgata atgacgataa ccggcgtcag gatcagggca
    13321 aaaatcatca ggctggcctg tacagtgtca gtccagctca ccgcgaggaa accgccaata
    13381 aaggtgtaaa ggatcgtcga cgcagctccc gccaacagag ccgtttcgta actcatgcca
    13441 aaggtacttt caaacagacg cgcgcctgcc acaatgcccg aagcgcaata aatggtgaag
    13501 aacagcaaaa taaccagcgc ggagataatg cgcaaaatgc ggcttttatc ttcaaaacgc
    13561 ccggtgaaat aatccggcaa tgttaaggcg ttattattgt attcggtatg cacacgcaac
    13621 cgcccggcca ccagcttcca gttgatccac gcgcctaatg tcaggccaat ggcgatccag
    13681 ctttcggaaa tcccggaaag aaaaaccgcg ccaggcaacc ccattaacag ccagccgctc
    13741 atatccgacg cacccgccga taatgccgtc acgaatggcc caagactacg accgcccaga
    13801 atatagtcgt caaagttttt cgttgatcgc caggcgataa acccaatcaa tatcatgcca
    13861 aagatataga cacaaaatgt caccaacatc ggtgtgctaa tagccatcta aagtctccaa
    13921 aaaattatta tcggcaatgt cgaaacttgc cgttatatct gccaccggaa cggggtaaca
    13981 gagtttatgt tttaccaggg cgaccgtatc ctgccggaag cgctggttat tcacaatcga
    14041 tttaacacac catttacatt aaattttagt gctcagcgac actatttttc atcaggttgc
    14101 actctctcac attttttgcg gttgcacctt tcaaaaaggt taactgccgc agagaaaaag
    14161 tctgagttat tttttaatca ctgtcatatc gatttctttt attaacattt cattcatttt
    14221 taagcttgct acgcaagtca catttaacat ggttgcacaa agttgcaaca tcatggatat
    14281 ttcacgataa cgttaagttg cacctttcag aacaacagga gtaatggcat gggaaccacc
    14341 accatggggg ttaagctgga cgacgcgacg cgtgagcgta ttaagtctgc cgcgacacgt
    14401 atcgatcgca caccacactg gttaattaag caggcgattt tttcttatct cgaacaactg
    14461 gaaaacagcg atactctgcc ggagctacct gcgctgcttt ctggcgcggc caatgagagc
    14521 gatgaagcac cgactccggc agaggaacca caccagccat tcctcgactt tgccgagcaa
    14581 atattgcccc agtcggtttc ccgcgccgcg atcaccgcgg cctatcgccg cccggaaacc
    14641 gaagcggttt ctatgctgct ggaacaagcc cgcctgccgc agccagttgc tgaacaggcg
    14701 cacaaactgg cgtatcaact ggccgataaa ctgcgtaatc aaaaaaatgc cagtggtcgt
    14761 gcaggaatgg tccaggggtt attgcaggag ttttcgctgt catcgcagga aggcgtggcg
    14821 ctgatgtgtc tggcagaagc gttgttgcgt attcccgaca aagccacccg cgacgcgtta
    14881 attcgcgaca aaatcagcaa cggtaactgg cagtcacaca ttggtcgtag cccgtcactg
    14941 tttgttaatg ccgctacctg ggggctgctg tttactggca aactggtttc cacccataac
    15001 gaagccagcc tctcccgctc gctgaaccgc attatcggta aaagcggtga accgctgatc
    15061 cgcaaaggtg tggatatggc gatgcgcctg atgggcgagc agttcgtcac gggtgaaacc
    15121 atcgcggaag cgttagccaa tgcccgcaag ctggaagaga aaggtttccg ttactcttac
    15181 gatatgctgg gcgaagctgc actgactgcc gcagacgcac aggcgtatat ggtttcctat
    15241 cagcaggcga ttcacgccat tggtaaagcg tctaacggtc gtggcatcta tgaaggtccg
    15301 ggcatttcaa tcaaactgtc ggcgctgcat ccgcgctaca gccgcgccca gtatgaccgg
    15361 gtaatggaag agctttaccc gcgtctgaaa tcactcaccc tgctggcgcg tcagtatgat
    15421 attggtatta acatcgatgc cgaagaggcc gatcgtctgg agatctccct cgatctgctg
    15481 gaaaatctct gtttcgagcc ggaactggca ggctggaacg gcatcggttt tgttattcag
    15541 gcttatcaaa aacgctgccc gttggtgatc gattacctgg ttgatctcgc cacccgcagc
    15601 cgtcgccgtc tgatgattcg cctggtgaaa ggcgcgtact gggatagtga aatcaagcgt
    15661 gcgcagatgg acggccttga aggctatccg gtttataccc gcaaggtgta taccgacgtt
    15721 tcttatctcg cctgtgcgaa aaagctgctg gcggtgccga atctaatcta cccgcagttc
    15781 gcgacgcaca acgcccatac gctggcagcg atttatcaac tggcaggtca gaactactat
    15841 ccgggtcagt acgagttcca gtgcctgcat ggtatgggcg agccactgta cgagcaggtc
    15901 accgggaaag ttgccgacgg taaacttaac cgaccgtgtc gtatttatgc cccggttggc
    15961 acacatgaaa cgctgctggc gtatctggtg cgtcgcctgc tggaaaacgg tgctaacacc
    16021 tcgtttgtta accgtattgc cgacacctct ttgccactgg atgaactggt cgccgatccg
    16081 gtcactgctg tagaaaaact ggcgcaacag gaagggcaaa ctggattacc gcatccgaaa
    16141 attcccctgc cgcgcgatct ttacggtcac gggcgcgaca actcggcagg gctggatctc
    16201 gctaacgaac accgtctggc ctcgctctcc tctgccctgc tcaatagtgc actgcaaaaa
    16261 tggcaggcct tgccaatgct ggaacaaccg gtagcggcag gtgagatgtc gcccgttatt
    16321 aaccctgcgg aaccgaaaga tattgtgggc tttgtgcgtg aagccacgcc acgtgaagta
    16381 gaacaggcgc tggaaagtgc ggttaataac gcgccaatct ggtttgccac gcctccggct
    16441 gaacgcgcgg cgattttgca tcgcgctgcc gtgctaatgg aaagccagat gcagcaactg
    16501 attggtattc tggtgcgtga agccggaaaa accttcagta acgccattgc cgaagtgcgc
    16561 gaagcggtcg attttctcca ctactacgcc ggacaggtgc gggatgattt cgctaacgaa
    16621 acccaccgtc cattagggcc tgtggtgtgt atcagtccgt ggaacttccc gctggctatt
    16681 ttcaccgggc agatcgctgc cgcactggcg gcaggtaaca gcgtgctggc aaaaccggca
    16741 gaacaaacgc cgctgattgc cgcgcaaggg atcgccattt tgctggaagc gggtgtaccc
    16801 cctggcgtgg tgcaattgct gccaggtcag ggggaaaccg tgggcgcgca actgacgggt
    16861 gatgatcgcg tgcgcggggt gatgtttacc ggttcaaccg aagtcgctac gttactgcag
    16921 cgcaatatcg ccagccgcct ggacgctcag ggtcgcccta ttccgctcat cgctgaaacc
    16981 ggcggcatga acgcgatgat tgtcgattct tcagcactga ccgaacaagt cgtcgtggat
    17041 gtgctggcct cggcgttcga cagtgcgggt cagcgttgtt cggcactgcg cgtgctgtgc
    17101 ctgcaagatg agattgccga ccacacgttg aaaatgctgc gcggtgcaat ggccgaatgc
    17161 cggatgggta atccgggtcg cctgaccacc gatatcggtc cggtgattga tagcgaagcg
    17221 aaagccaata ttgagcgcca tattcagacc atgcgtagca aaggccgtcc ggtgttccag
    17281 gcggtgcggg aaaacagcga agatgcccgt gaatggcaaa gcggcacctt tgtcgccccg
    17341 acgctgatcg aactggatga ctttgccgaa ttgcacaaag aggtctttgg tccggtgctg
    17401 catgtggtgc gttacaaccg taaccagcta ccagagctga tcgagcagat taacgcttcc
    17461 ggctatggtc tgacgcttgg tgtccatacg cgtattgatg aaaccatcgc ccaggtcact
    17521 ggctcggccc atgttggtaa cctgtatgtt aaccgtaata tggtgggcgc agtggttggt
    17581 gtgcagccgt tcggcggcga agggttgtcc ggtaccgggc cgaaagcagg cggtccgctc
    17641 tatctctacc gtctgctggc gaatcgcccg gaaagtgcgc tggcagtgac gctcgcgcgt
    17701 caggatgcag agtatccggt cgatgcacag ttgaaagccg cattgactca gccgctaaat
    17761 gcactgcggg aatgggcagc aaatcgtcca gaattgcagg cgttatgtac gcaatatggc
    17821 gagctggcgc aggcaggaac acaacgattg ctgccggggc cgacgggtga acgcaacacc
    17881 tggacgctgc tgccgcgtga gcgcgtgttg tgtattgccg atgatgagca ggatgcgctg
    17941 actcagctcg ccgccgtgct ggcggtgggc agccaggtac tgtggccgga tgacgcgctg
    18001 catcgtcagc tagtaaaggc attgccatcg gcagtcagcg aacgtattca actggcgaaa
    18061 gcggaaaata taaccgctca accctttgat gcggtgatct tccacggtga ttcggatcag
    18121 cttcgcgcat tgtgtgaagc cgttgctgcg cgggatggcg caattgtttc ggtgcagggt
    18181 tttgcccgtg gcgaaagcaa tatccttctg gaacggctgt atatcgaacg ttcgctgagt
    18241 gtgaataccg ctgccgctgg cggtaacgcc agtttaatga cgatcggtta aacaggtagc
    18301 cggaggatgt tacaacctcc tccggcatct ttaacgtggt cgaatcccct caataataat
    18361 ccgctgcacg ttttcaaccg tttgattgaa aaatatctca tcgcgcaacg tcgcgcctgt
    18421 caccgcctcc acctgagggg cgaaatcggc gtaatgttga gtggaagccc aaatcatgaa
    18481 aatcaaatgc tgcggatcaa tcggcgcgag tttgccgctt ttgacccaac cggcaatcag
    18541 cgcagatttc tcatcaatta atgccttcaa atcgcccgtc agttcatcca ttaacagcgg
    18601 cgcgcctgcc agcatctcca tacagaacag gcgcgaagcc tgcggataat cgcgggagac
    18661 ttccagcttc agacggatgt actcttttat cgccgccagc ggagcgaaat cttcacgaaa
    18721 cgcttttaac ggtgccagcc agatatcgag aatctgccgc agcacggcaa tatacagcgc
    18781 ctcttttgac ggaaagtaat acagcagatt ggtttttgaa acacccgcca actctgcgat
    18841 ctgctccagc cttgtgccgt gaaaaccgaa ttgtgaaaaa gtgtccagtg ctgcgctaag
    18901 aatcgctttt ttcttcgcgc ttactgcgcg cgaacgttta cccgttgttt tcactgcgcc
    18961 ttgcgtcatg cgctctcccc tcttgtagat tggcaacagg atagcaaaac ccccgcccgg
    19021 cctcgacctt ctgcactctc atcgcgcaca gatgcatgtt ttatgtgcaa ctgttttgac
    19081 cgtttagtcc actttttatc agatatttta gataacgtaa tattaacata ttaataacat
    19141 taacttttta aaaactggca tccgctttgc aaacaagcca atacgcggct gaaaaggata
    19201 tgcaggatgc agcgccccgt ctgactttca ctttacgaga cgaagagagg ttgatgatga
    19261 aaattggcgt attcgtacct attggcaaca acggctggct catttcgacc cacgcgccgc
    19321 agtacatgcc gacctttgaa ctgaataaag ccattgtgca gaaagcggag cattaccatt
    19381 tcgatttcgc cctgtcgatg atcaaactgc gtggctttgg cggcaaaact gagttctggg
    19441 atcacaacct tgagtcgttc accttgatgg cgggtctggc agccgtgacc tcgcgcattc
    19501 agatttacgc cactgctgcc accttaacgt tacctccggc aatcgtcgcc cgtatggccg
    19561 caaccatcga ctccatctct ggcgggcgtt ttggcgtcaa cctcgtgact ggctggcaaa
    19621 agcccgtgta tgagcagatg ggtatctggc ctggcgatga ctatttctcc cgtcgttacg
    19681 actatctcac cgaatatgtt caggtgctgc gcgacctgtg gggcacgggg aaaagcgatt
    19741 t
//
LOCUS       DADMKF010000070        19450 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.071, whole genome shotgun sequence.
ACCESSION   DADMKF010000070 DADMKF010000000
VERSION     DADMKF010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19450
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.071"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            <1..245
                     /locus_tag="JNC24_002291"
     CDS             <1..245
                     /locus_tag="JNC24_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HAY9159023.1"
                     /translation="AHADYPGDEYQFGKEIAESELLWPVDRVQKSLNGELQQINGADY
                     FIFGHMMFDNIQTFANQIYIDTGTPKSGRLSFYKIK"
     gene            complement(296..1093)
                     /locus_tag="JNC24_002292"
     CDS             complement(296..1093)
                     /locus_tag="JNC24_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAY9159024.1"
                     /translation="MSAGQRKEQKLIPVERRQIILEMVAEKGIVSIAELTDRMNVSHM
                     TIRRDLQKLEQQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVQP
                     GSCIYLDAGTTTLAIAQHLIHMESLTVVTNDFVIANYLLDNSNCTIIHTGGAVCRENR
                     SCVGEAAATMLRSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDA
                     TKYGQVATWLALPLSEFDQIITDDGLPESASRALAKQDLSLLVAKNE"
     gene            1259..2167
                     /locus_tag="JNC24_002293"
     CDS             1259..2167
                     /locus_tag="JNC24_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HAY9159025.1"
                     /translation="MKTGSEFHVGIVGLGSMGMGAALSCVRAGLSTWGADLNSNACAT
                     LKEAGACGVSDNAATFAEKLDALLVLVANAAQVKQVLFGETGVAQHLKPGTAVMVSST
                     IASADAQEIATALAGFDLEMLDAPVSGGAVKAANGEMTVMASGSDIAFERLAPVLEAV
                     AGKVYRIGAEPGLGSTVKIIHQLLAGVHIAAGAEAMALAARAGIPLDVMYDVVTNAAG
                     NSWMFENRMRHVVDGDYTPHSAVDIFVKDLGLVADTAKALHFPLPLASTALNMFTSAS
                     NAGYGKEDDSAVIKIFSGITLPGAKS"
     gene            2164..3426
                     /locus_tag="JNC24_002294"
     CDS             2164..3426
                     /locus_tag="JNC24_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HAY9159026.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALSWLQQQGCKQIYFKYCSTFDSTAKGNIGPVTDAL
                     MDALDTPFTVFSPALSVNGRTVYQGYLFVMNQLLAESGMRHHPVNPMTDSYLPRLVEA
                     QSTGRCGVVSAHVFEQGVDAVRQELARLQQEGYRYAVLDALTEHHLEIQGEALRDAPL
                     VTGGSGLAIGLARQWAQENGNQAREAGRPLAGRGVVLSGSCSQMTNRQVAHYRQIAPA
                     REVDVARCLSTETLAAYAHELAEWVLGQESVLAPLVFATASTDALAAIQQQYGAQKAS
                     QAVETLFSQLAARLAAEGVTRFIVAGGETSGVVTQSLGIKGFHIGPTISPGVPWVNAL
                     DKPVSLALKSGNFGDEAFFSRAQREFLS"
     gene            3423..4061
                     /locus_tag="JNC24_002295"
     CDS             3423..4061
                     /locus_tag="JNC24_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HAY9159027.1"
                     /translation="MSDFAKVEQSLREEMTRIASSFFQRGYATGSAGNLSLLLPDGNL
                     LATPTGSCLGNLDPQRLSKVAADGEWLSGDKPSKEVLFHLALYRNNPRCKAVVHLHST
                     WSTALSCLQGLDSSNVIRPFTPYVVMRMGNVPLVPYYRPGDKRIAQDLAELAADNQAF
                     LLANHGPVVCGESLQEAANNMEELEETAKLIFILGDRPIRYLTAGEIAELRS"
     gene            4066..4842
                     /locus_tag="JNC24_002296"
     CDS             4066..4842
                     /locus_tag="JNC24_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HAY9159028.1"
                     /translation="MPRFAANLSMMFTEVPFIERFAAARKAGFDAVEFLFPYDYSTLQ
                     IQKQLEQNHLTLALFNTAPGDINAGEWGLSALPGREHEAHADIDLALEYALALNCEQV
                     HVMAGVVPAGEDAERYRAVFIDNLRYAADRFALHGKRILVEALSPDVKPHYLFSSQYQ
                     ALAIVEEVARDNVFIQLDTFHAQKVDGNLTHLIRDYAGKYAHVQIAGLPDRHEPDDGE
                     INYPWLFRLFDEVGYQGWIGYEYKPRGLTEEGLGWFDAWR"
     gene            4931..6295
                     /locus_tag="JNC24_002297"
     CDS             4931..6295
                     /locus_tag="JNC24_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family transporter"
                     /protein_id="HAY9159029.1"
                     /translation="MSTITLLCIALAGVIMLLLLVIKAKVQPFVALLLVSLLVALAAG
                     IPAGEVGKVMIAGMGGVLGSVTIIIGLGAMLGRMIEHSGGAESLANYFSRKLGDKRTI
                     AALTLAAFFLGIPVFFDVGFIILAPIIYGFAKVAKISPLKFGLPVAGIMLTVHVAVPP
                     HPGPVAAAGLLHADIGWLTIIGIAISIPVGIVGYFAAKIINKHQYAMSVEVLEQMQLA
                     PASEEGATKLSDKINPPGVALVTSLIVIPIAIIMAGTVSATLMPPSHPLLGTLQLIGS
                     PMVALMIALVLAFWLLALRRGWSLQHTSDIMGSALPTAAVVILVTGAGGVFGKVLVES
                     GVGKALANMLQMIDLPLLPAAFIISLALRASQGSATVAILTTGGLLSEAVMGLNPIQC
                     VLVTLAACFGGLGASHINDSGFWIVTKYLGLSVADGLKTWTVLTTILGFTGFLITWCV
                     WAVI"
     gene            complement(6389..7459)
                     /gene="rpoS"
                     /locus_tag="JNC24_002298"
     CDS             complement(6389..7459)
                     /gene="rpoS"
                     /locus_tag="JNC24_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HAY9159030.1"
                     /translation="MFRQGITGRSHLMSQNTLKVHDLNEDAEFDENGVEVFDEKALVE
                     EEPSDNDLAEEELLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALLTAEEE
                     VYFARRALRGDVASRRRMIESNLRLVVKIARRYGNRGLALLDLIEEGNLGLIRAVEKF
                     DPERGFRFSTYATWWIRQTIERAIMNQTRTIRLPIHIVKELNVYLRTARELSHKLDHE
                     PSAEEIAEQLDKPVDDVSRMLRLNERITSVDTPLGGDSEKALLDILADEKENGPEDTT
                     QDDDMKQSIVKWLFELNAKQREVLARRFGLLGYEAATLEDVGREIGLTRERVRQIQVE
                     GLRRLREILQTQGLNIEALFRE"
     gene            complement(7486..8625)
                     /gene="nlpD"
                     /locus_tag="JNC24_002299"
     CDS             complement(7486..8625)
                     /gene="nlpD"
                     /locus_tag="JNC24_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HAY9159031.1"
                     /translation="MSAGSPKFTVRRIAALSLVSLWLAGCSDTSNPPAPVSSVNGNAP
                     ANTNSGMLITPPPKMGTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENG
                     RIVYNRQYGNIPKGSYSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVG
                     QTLQVGNASGTPITGGNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSA
                     NKMLPNNKPTATTVTAPVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASE
                     GGNKGIDIAGSKGQAIIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLV
                     REQQEVKAGQKIATMGSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            complement(8765..9391)
                     /gene="pcm"
                     /locus_tag="JNC24_002300"
     CDS             complement(8765..9391)
                     /gene="pcm"
                     /locus_tag="JNC24_002300"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HAY9159032.1"
                     /translation="MVSRRVQALLEQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            complement(9385..10146)
                     /gene="surE"
                     /locus_tag="JNC24_002301"
     CDS             complement(9385..10146)
                     /gene="surE"
                     /locus_tag="JNC24_002301"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HAY9159033.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            complement(10127..11176)
                     /gene="truD"
                     /locus_tag="JNC24_002302"
     CDS             complement(10127..11176)
                     /gene="truD"
                     /locus_tag="JNC24_002302"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HAY9159034.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHAREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGAQRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEK
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            complement(11173..11652)
                     /gene="ispF"
                     /locus_tag="JNC24_002303"
     CDS             complement(11173..11652)
                     /gene="ispF"
                     /locus_tag="JNC24_002303"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HAY9159035.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            complement(11652..12362)
                     /gene="ispD"
                     /locus_tag="JNC24_002304"
     CDS             complement(11652..12362)
                     /gene="ispD"
                     /locus_tag="JNC24_002304"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HAY9159036.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVKRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDAQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            complement(12381..12692)
                     /gene="ftsB"
                     /locus_tag="JNC24_002305"
     CDS             complement(12381..12692)
                     /gene="ftsB"
                     /locus_tag="JNC24_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HAY9159037.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAAQQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            complement(12886..13209)
                     /locus_tag="JNC24_002306"
     CDS             complement(12886..13209)
                     /locus_tag="JNC24_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HAY9159038.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            complement(13259..13864)
                     /gene="cysC"
                     /locus_tag="JNC24_002307"
     CDS             complement(13259..13864)
                     /gene="cysC"
                     /locus_tag="JNC24_002307"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HAY9159039.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            complement(13864..15291)
                     /gene="cysN"
                     /locus_tag="JNC24_002308"
     CDS             complement(13864..15291)
                     /gene="cysN"
                     /locus_tag="JNC24_002308"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HAY9159040.1"
                     /translation="MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGPTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEAFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAAPSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            complement(15293..16201)
                     /gene="cysD"
                     /locus_tag="JNC24_002309"
     CDS             complement(15293..16201)
                     /gene="cysD"
                     /locus_tag="JNC24_002309"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HAY9159041.1"
                     /translation="MDQIRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            16453..17490
                     /gene="iap"
                     /locus_tag="JNC24_002310"
     CDS             16453..17490
                     /gene="iap"
                     /locus_tag="JNC24_002310"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HAY9159042.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKSWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGMDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTAAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     gene            complement(17758..18051)
                     /gene="cas2"
                     /locus_tag="JNC24_002311"
     CDS             complement(17758..18051)
                     /gene="cas2"
                     /locus_tag="JNC24_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endoribonuclease
                     Cas2"
                     /protein_id="HAY9159043.1"
                     /translation="MSMVVVVTENVPPRLRGRLAIWLLEVRAGVYVGDTSKRIREMIW
                     QQITQLAGCGNVVMAWATNIESGFEFQTWGENRRIPVDLDGLRLVSFLPVDNQ"
     gene            complement(18048..18971)
                     /gene="cas1e"
                     /locus_tag="JNC24_002312"
                     /pseudo
     CDS             complement(18048..18971)
                     /gene="cas1e"
                     /locus_tag="JNC24_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311636.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endonuclease Cas1"
     gene            complement(18968..>19450)
                     /gene="cas6e"
                     /locus_tag="JNC24_002313"
     CDS             complement(18968..>19450)
                     /gene="cas6e"
                     /locus_tag="JNC24_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HAY9159044.1"
                     /translation="RFFVLSQERPAESDIFTIECRPFAPELRTGQSLCFNLRANPTIC
                     KAGKRHDLLMEAKRQVRGQVEGSDVWLHQQQAALDWLAAQGERSGFTLLDTSVDAYRQ
                     QQLRRENSRQLIQFSSVDYTGMLTVTAPGLFLQRLSQGYGKSRAFGCGLMLIKPGAEA
                     "
BASE COUNT         4602 a         5243 c         4858 g         4747 t
ORIGIN      
        1 tcgcacatgc agattatccg ggtgatgaat atcagtttgg taaagaaata gcagaaagcg
       61 aattactctg gcctgttgat cgtgtgcaga aatcgcttaa tggcgagtta caacaaataa
      121 acggcgctga ttattttata tttggacata tgatgtttga taacattcag acgttcgcta
      181 accagattta tattgatacc ggcacgccga aaagcgggcg gctgtcattt tataaaataa
      241 agtagtctca tgcttcttct gtgaagcatg agtaaccaaa tgttattgca ggccattatt
      301 catttttcgc taccagcaaa gagagatcct gcttcgccag cgcgcgactg gcactctccg
      361 gcaaaccgtc gtctgtgata atctgatcaa actcgcttaa tggtaacgcc agccatgtcg
      421 ccacctgacc atatttcgtc gcatcacaga ccaaaactcg ctggcggctg gcactggcaa
      481 tcgcccgttt caccgtgact ttatcttccg ctggcgtaga aatcccccgc acactccatg
      541 acgatgcaga aataaaagcc tgatcaatca tcaggctgcg cagcatggtc gcagcggctt
      601 ccccgacaca ggaacggttt tcccgacaca ctgcaccgcc agtgtgaata attgtgcaat
      661 tactgttgtc gagcaagtag ttcgcaataa cgaaatcgtt tgtgaccaca gtcagtgact
      721 ccatgtgaat cagatgctgt gctatcgcta acgtggtcgt tcccgcatcc agatagatac
      781 aacttcccgg ctgaacaaga cttgccgcca gcttgccaat agccgctttt tgcgtcattg
      841 ccagcgcagt ttttacctga tgagaaggtt catgcgccac gcgtcccgga gactggacgc
      901 ctccggacac cagcacaacg gctccctgct gctccagttt ttgtaaatcc cgacgaatgg
      961 tcatatgtga cacattcatt ctgtccgtta gttcagcaat actgacaatg cctttttcag
     1021 ctaccatctc aaggatgatt tggcgacgct ctacgggtat caacttttgc tccttccttt
     1081 gtcctgctga cattctacgc tatttgcctg cgaaacgtgc gcggcgcaac taacgcttag
     1141 ttcacataaa ataacacaca atgttaattt atgtgaatca gctcaccata tcgttatctt
     1201 ccagcgctta tattcacaat atcaaacaaa atatcactta aattaacaat gagagcagat
     1261 gaaaacggga tctgagtttc atgtcggtat cgttggctta gggtcaatgg gaatgggagc
     1321 agcactgtca tgtgtccgcg caggtctttc tacctggggc gcagacctga acagcaatgc
     1381 ctgcgctacg ttgaaagagg caggtgcttg cggggtttct gataacgccg cgacgtttgc
     1441 cgaaaaactg gacgctctgc tggtgctggt ggccaatgcg gcccaggtta aacaggtgct
     1501 gtttggtgaa acaggcgttg cacaacatct gaaacccggt acggcagtaa tggtttcttc
     1561 cactatcgct agtgctgatg cgcaagaaat tgctaccgct ctggctggat tcgatctgga
     1621 aatgctggat gcgccagttt ctggtggtgc agtaaaagcc gctaacggtg aaatgactgt
     1681 catggcctcc ggtagcgata ttgcctttga acgactggca cccgtgctgg aagccgttgc
     1741 cggaaaagtt tatcgcatag gtgcagaacc gggactaggt tcgaccgtaa aaattattca
     1801 ccagttgtta gcgggcgtac atattgctgc cggagccgaa gcgatggcac ttgcagcccg
     1861 tgcggggatc ccgctggatg tgatgtatga cgtcgtgacc aatgccgccg gaaattcctg
     1921 gatgttcgaa aaccggatgc gtcatgtggt ggatggcgat tacaccccgc attcagccgt
     1981 cgatattttt gttaaggatc ttggtctggt tgccgataca gccaaagccc tgcacttccc
     2041 gctgccattg gcctcaacag cattgaatat gttcaccagc gccagtaacg cgggttacgg
     2101 gaaagaagac gatagcgcag ttatcaagat tttctctggc atcactctac cgggagcgaa
     2161 atcatgatca agattggcgt tatcgccgat gattttaccg gcgcgacgga tatcgccagt
     2221 tttctggtgg aaaacggtct accaacggta caaattaacg gtgttccaac aggtaaaatg
     2281 ccggaagcaa tcgacgcact ggtgatcagc ctgaaaacgc gctcctgtcc ggtggttgaa
     2341 gccacacagc aatcgctggc ggctctgagc tggttgcaac agcaaggttg caaacagatc
     2401 tatttcaaat actgctctac tttcgacagt acggcgaaag gtaatattgg cccggttacc
     2461 gatgccttaa tggatgctct cgacacgccg tttacggtct tctctccggc cctgtcggtc
     2521 aacggacgta cggtttatca ggggtatttg ttcgtaatga atcaactgct ggccgaatcc
     2581 gggatgcgcc atcacccggt aaatcccatg accgacagct atcttccccg tctggttgaa
     2641 gcgcaatcca cagggcgctg cggcgtcgtt tcggcacatg ttttcgaaca aggcgtggat
     2701 gccgttcgtc aagagctggc tcgcttacag caagagggct accgctacgc ggtgcttgat
     2761 gcgctgaccg aacaccatct ggaaattcag ggagaagcct tgcgcgatgc cccactggta
     2821 acgggcggtt ctggtctggc gattggcctg gcccggcagt gggcgcaaga aaacggtaac
     2881 caggctcgcg aagcagggcg tccgctcgct gggcgcggcg tagtgctctc cggttcatgc
     2941 tctcaaatga ccaaccgcca ggtggcacat taccgtcaaa ttgcaccagc ccgtgaagtt
     3001 gatgtggcac gctgcctctc aaccgaaact ctggccgctt atgcacacga actggcagag
     3061 tgggttctgg gccaggaaag tgtacttgct ccactggttt ttgccaccgc cagcactgac
     3121 gcattggcag caattcaaca gcaatacggt gcacaaaaag ccagtcaggc agtagaaact
     3181 ctgttttctc aactagcggc gcggttagca gcggaaggcg tgacacgctt tattgtcgca
     3241 ggcggtgaga cctccggcgt agtcacacag agcctgggca taaaagggtt tcatattggc
     3301 ccaaccattt cccccggcgt gccgtgggta aacgcactgg ataagcctgt ctcactcgcc
     3361 cttaaatctg gcaacttcgg tgatgaagcc tttttttcac gagcccaaag agagttttta
     3421 tcatgagcga tttcgcaaaa gtagagcagt ctttgcgaga ggagatgacg cggattgcca
     3481 gttcattctt tcagcgcggc tatgcaaccg gttcggctgg caatctgtcg ctgcttttac
     3541 ctgacgggaa tttactggcg acaccgacag gttcatgcct gggcaatctc gatccgcagc
     3601 ggctttccaa agtcgccgcg gatggcgaat ggttaagtgg tgacaaaccc tcgaaagagg
     3661 tgctctttca tctggcgctg tatcgcaaca atccgcgctg taaagcggtg gtgcatttgc
     3721 acagcacatg gtcgacggcg ctttcctgcc tgcaagggct ggacagcagc aacgttattc
     3781 gtccgttcac accatacgtg gtgatgcgga tgggaaatgt cccgctggtg ccttattacc
     3841 gaccgggcga taaacgcatc gcacaggatc tggcggaact ggcagcagac aatcaggctt
     3901 ttttactggc aaatcatggc ccagtggttt gcggtgaaag cctgcaagaa gccgccaaca
     3961 atatggaaga gctggaggaa acggcaaagc tgatttttat tctcggtgac cgcccgatcc
     4021 gttatctgac cgcaggtgaa attgcggaat taaggagtta atgcaatgcc tcgttttgca
     4081 gctaatttat ccatgatgtt caccgaagtg ccttttattg aacgcttcgc cgcagcgcga
     4141 aaagccgggt tcgatgctgt ggaatttctg tttccctatg actactccac cctgcaaatc
     4201 caaaagcaac tggagcaaaa ccatctgaca ctggcgctgt tcaataccgc tcccggagat
     4261 attaatgcgg gggaatgggg attatccgcc cttcccggac gtgagcacga agcacacgca
     4321 gacatcgacc tggcactgga atatgcattg gcgctcaatt gtgaacaagt ccatgtgatg
     4381 gcaggcgtcg tgcccgctgg cgaagacgct gaacggtacc gggcagtatt tatcgataat
     4441 ctccgctacg ctgccgaccg ctttgcgcta cacggcaagc gaatattagt tgaagcactc
     4501 agccccgacg ttaagcctca ttatctcttc tccagccagt atcaggcact ggctattgtt
     4561 gaagaggttg cgcgagataa cgtgtttatt caactagata cttttcatgc gcaaaaagta
     4621 gatggcaacc tgacgcattt aatccgcgac tacgctggga aatatgcgca cgtacaaatt
     4681 gccggactac ctgatcgaca tgaaccggac gatggagaaa tcaactatcc gtggctgttc
     4741 cgcctgttcg atgaggtggg atatcagggc tggatcggtt atgaatataa acctcgtggc
     4801 ctcaccgaag aagggcttgg ctggtttgac gcctggcgct aattcgttaa atccctaatt
     4861 acaacgtacc catacatccc cccatctacg gatggggtag gttaactata actttcagac
     4921 agggtttccc atgtccacaa ttacattgtt atgcattgcg ttagctggcg taatcatgct
     4981 gttgctgctg gtcatcaagg caaaggtaca accattcgtt gctctgctcc tcgtcagcct
     5041 gttagtcgca cttgcggcgg gtataccggc gggcgaagtg ggtaaagtga tgatcgccgg
     5101 gatgggcggt gtgcttggtt ccgtcactat tattattggt ctgggcgcta tgctggggcg
     5161 tatgatcgaa cactctggtg gtgcagagtc actggctaat tatttcagtc gcaagttagg
     5221 tgacaaacga actatcgctg cgctgactct ggcagcgttc ttcctcggta ttcccgtctt
     5281 ctttgatgtc ggctttatta ttcttgctcc aatcatttac ggttttgcca aggttgccaa
     5341 aatatcgcca ctcaaatttg gcctgcctgt cgctgggatc atgctaactg tacatgtggc
     5401 ggtaccgccg catccaggcc ctgtcgccgc agcggggtta ctccacgcag acatcggctg
     5461 gctaaccatc atcggtattg cgatttctat tcccgtaggg attgttggct actttgcagc
     5521 gaaaataatc aataagcacc aatatgcgat gtcggtggaa gtactggaac agatgcaact
     5581 ggctccggcc agtgaggaag gcgcgacaaa attaagcgat aaaataaatc caccgggcgt
     5641 cgcgctggtc acctcgctaa ttgttattcc tatcgcgatt atcatggcgg gtacggtttc
     5701 cgcaacactg atgccgcctt cgcatcccct gcttggtacg ctacagctga tcggctcacc
     5761 aatggtagct ctaatgattg cgctggtgct ggcattctgg ttattggctt tacgtcgcgg
     5821 ctggagctta caacacacca gcgacattat gggctcagcc cttcccactg cggcggtagt
     5881 gattttggtt actggtgctg gaggggtatt tggcaaagtg ctggtggaat cgggcgtcgg
     5941 caaagccctt gccaacatgc tacaaatgat tgacctgcct ctgttaccag ccgcatttat
     6001 tatttcactg gcgctgcgtg catcgcaggg gtcagcaacc gtagcaatcc tgacaaccgg
     6061 cgggttactc tcagaagcgg tgatgggatt gaatccgatt cagtgcgtat tggtgacgct
     6121 ggcagcctgc tttggtgggc ttggggcgtc acatattaat gactcagggt tctggattgt
     6181 gaccaaatat ctggggttgt cggtagcaga cggtctgaaa acctggacag tgttaacgac
     6241 cattctcggt tttaccggat tcttaattac ctggtgcgta tgggcggtaa tttgacctta
     6301 ataaaaaggt ccgatgagca tcggaccttt tattgtgcac agaaaaggcc agcctcgctt
     6361 gagactggcc tttctgacag atgcttactt actcgcggaa cagcgcttcg atattcagcc
     6421 cctgcgtttg caggatttca cgcaaacggc gcaggccttc aacctgaatc tggcgaacac
     6481 gttcacgggt gaggccaatt tcacgaccta catcttccag tgttgccgct tcgtacccca
     6541 gcaaaccgaa tcgacgtgcc agtacttcac gctgtttggc gttcagctcg aacagccatt
     6601 tgacgatgct ctgcttcata tcgtcatctt gcgtggtatc ttccggaccg ttctcttttt
     6661 catcggccag gatgtccagc aacgcttttt cggaatcacc acccagcggg gtgtctaccg
     6721 aggtaatgcg ctcgttaaga cgaagcatac ggctgacgtc atcaactggc ttatccagtt
     6781 gctctgcgat ctcttccgca cttggttcat ggtccagctt atgggacaac tcacgtgcgg
     6841 ttcgcaggta aacgttcagc tcctttacga tgtgaatcgg caaacgaata gtacgggttt
     6901 ggttcataat cgcccgttca atcgtctggc gaatccacca agttgcgtat gttgagaagc
     6961 ggaaaccacg ttccgggtca aacttctcta ccgcgcggat cagccccagg ttgccctctt
     7021 cgataaggtc cagcaacgcc agaccacgat tgccataacg gcgggcaatt tttaccacca
     7081 gacgcaagtt actctcgatc atccggcggc gagaggcgac atctccacgc agtgcgcgac
     7141 gcgcaaaata aacttcttct tcggccgtta gcagtgcgcg acgcgcaaaa taaacttctt
     7201 cttcggccgt taacagtggt gaataaccaa tctcaccaag gtaaagctga gtcgcgtcca
     7261 acacacgctg tgtggctccc tgcgataaca gttcctcttc ggccaaatcg ttatcactgg
     7321 gttcctcttc tactaaggcc ttttcgtcaa aaacctcaac tccgttctca tcaaattccg
     7381 catcttcatt taaatcatga actttcagcg tattctgact cataaggtgg ctcctacccg
     7441 tgatcccttg acggaacatt caagcaaaag cctggttccg ccgatttatc gctgcggcaa
     7501 ataacgcagc gggtttacgg atttcccctt gtaacgaatt tcaaaatgca agcgtgttga
     7561 actggttccg gtgctaccca tggttgctat tttttgcccc gccttcactt cttgttgttc
     7621 ccggaccagc attgtgtcgt tatgggcgta ggcactcagg taatcatcat tatgtttgat
     7681 gataatcaga ttaccgtagc cgcgcagcgc gttaccggca taaacaacgc ggccatctgc
     7741 ggtcgcgata attgcctgtc ctttgctgcc tgcgatatca atccccttgt tgcccccctc
     7801 agaagcgcca aaggtttcga tcactttgcc ctcagtcggc cagcgccagg tggagatagg
     7861 cgtactggtt gatgtactgc tgacagtcgg ctcggttgtg cttgctgttg gtaccgttac
     7921 aggcgctgtg actgtggtcg cagttggctt gttgttcggc aacattttgt tagcactctg
     7981 ttcacccgaa gactcagaat acgtaattgt cggttgcgac gcaacagcaa cggtggaatt
     8041 ttgtgcaggc ttgatcacaa ctccttgctc tgctgcgtcg gcctgggtaa tggcatttcc
     8101 gccagtgatt ggcgtaccgg aagcattacc cacctgcaag gtctgaccaa cgttcagcgc
     8161 gtatggtgcc tgaatattgt tgcgctgagc aaggtcacgg aaatcgttgc cagtaatcca
     8221 ggcgatatag aaaagtgtgt cgcctttttt cacggtatag gtactgccgc tataactgcc
     8281 tttcggaatg ttcccatact gacggttata gacgatgcgt ccgttttcca tctgtaccgg
     8341 ctgctgagct actggctgca ctggctggat ttgcggttgt tgagtagcct gaatttgtgg
     8401 ctgctgtacc ggctgaattt gcggttgctg ggctgtagac gtcgtcccca ttttcggcgg
     8461 cggcgtaatc aacataccag aattagtatt tgcaggcgca ttgccattaa cggagctgac
     8521 cggtgccggt ggatttgaag tgtcagaaca gcctgccagc catagcgaaa ccagtgacaa
     8581 agcagcaatg cggcgaacgg tgaattttgg gcttcccgcg ctcatttatc ccccaggaaa
     8641 aattggttaa taaccagtga cataattacc gtgcaaggca ccctactgaa cactggaaaa
     8701 gatgttcacg atacgctgac ctgcggcaaa ataaccagga aaaatccagg tatttcctca
     8761 cgttttaagc cagctcaccc ttcactaaag ggacaaagcg cacggcctcc acggtatcga
     8821 taataaattc gcctccccga cgacgcaccc gtttcaaata ctggtgctcc tcccctacgg
     8881 gtaagacgag aatcccgcct tcgtccagct gcgtcattag cgcagttgga atttccggcg
     8941 gtgccgccgt aacaatgata gcgtcaaacg gcgcacgtgc ctgccaacct tgccatccat
     9001 cgccatgacg ggttgaaaca ttatgtaaat caagattttt caggcggcga cgtgcctgcc
     9061 actgcaagcc tttaatccgt tcaaccgagc aaacatgctg gacaagatgc gctaggattg
     9121 ccgtttgata tcccgaacca gtgccaattt ccagcacccg cgactgcggc gtcagctcga
     9181 gtaattcggt cattcgcgcc accatatacg gctgcgaaat tgtctgcccc tgacctatcg
     9241 gcaaggcgat attgtcccag gctttttgtt caaacgcttc atcaacgaat ttttcacgcg
     9301 gcacggcggc aagtgcattc agcacctgct catcctgaat accttgcgca cgtaattgct
     9361 ccagaagtgc ttgtacgcgt ctgcttacca ttgcgtgcca actcccacgc tgtttaacca
     9421 gtctgaaacc acatcttgcg cgctatgcgc agttaaatcc acatgcagcg gcgtgatgga
     9481 gacatagccc tcatctaccg cagcaaaatc ggtccccgga ccggcatcac atttaccgcc
     9541 cggcgggcca atccagtaca gcgtattgcc gcgcggatct tgctgcggga tcacctgatc
     9601 tgccggatgt cgtgtaccgc agcgcgtcac gcgaatacct ttgatttgat ccaagggtaa
     9661 atccggaacg ttaatattaa gaatacgtcc ggtgcgcagc ggctctttac acagtgcgcg
     9721 caaaattgaa caggttaccg ccgcggcagt gtcgtaatgt ttatgcccgt caagcgagac
     9781 ggccagcgcc ggaaaaccta aatgacggcc ttccatcgcg gcggctaccg taccggaata
     9841 aataacgtca tcccccagat tcggcccggc gttaatgccg gacacaacaa tgtccgggcg
     9901 cggacgcatc agagcattca cgccaagata gacgcaatcg gtcggggttc ccatttgcac
     9961 agcaatatca ccattttcaa aggtaaacgt gcgcagggag gattccagtg tcagagaatt
    10021 tgaagcgccg ctgcggttac gatcgggggc gaccacctga acgtcagcaa actcacgcaa
    10081 ggctttcgcc agcgtttgta taccgggtgc atgtaccccg tcatcattac tcagcaatat
    10141 gcgcataatc acctgttgtg ttgataagtt ccctgacaac gctggttgca aaactacccg
    10201 ccggaagcca gaaacggatc tctacggtga cgtcatccca ccaattccag cttaattgtt
    10261 gcggatacag caacatcgct ctgcgcgcgg cttcaacttt ttcgcgcacc agtaaagctt
    10321 gtaattcagt ttctgcggcg acagctgcct tttcgaatgc cagcgcttca cgctgagttc
    10381 cccattcgcc actacccggc aatgcggcgg ttatcatcag ctctttatcg ttgacgcgac
    10441 gctgtaattc cgccagttct tcggtggttg cgacaaacca gctaccacgt ccggctaatt
    10501 gtagcgcatc gccgtcaaca acttgattaa cgtctgcttt tttgaggcgc tcagcaacaa
    10561 tctgattaaa caacgcactg cgggctgccg acaaccaaaa actccgttta ttgcgatcgc
    10621 gcaccggagt attggtttgc gcccagcgct gcgccccctg caagttgcta ccgccaatcc
    10681 caaaacgttg ggcaccgaag tagttcggta caccttttac gcaaatatcg atcagacgtt
    10741 gttcaacgtc atcgcgattg ctcacttcgc gcagaaccag ggtaaaggcg ttacctttca
    10801 gcgcgcctaa acgcagcttg cgcttgtgcc gcgcatactc cagcacctgg cagccttcca
    10861 gttgaaaggc gctcagatcg ggcatttcct tgcctggcac gcgagcgcat aaccactgtt
    10921 ccgtaacagc atgtttgtct ttttgcccag cgaagctgac ttcacgggca tgaattttca
    10981 ggaatttcgc cagtgcatcc gccacaaaac gggtattgca gccgtttttg aggattctaa
    11041 ccagaatatg ctcaccttca ccatcaggct caaagcccaa atcttccacc accacaaagt
    11101 cttccggatt ggctttcagc agcccggtgc cttgcggttt accgtggagg taagtgagat
    11161 tatcaaactc aatcattttg ttgccttaat gagtagcgcc accgcttcac aggcaatccc
    11221 ttccccacgt ccggtaaatc caagtttttc cgtagtagtg gctttcacgt taacatcatc
    11281 catatggcag ccgagatctt cggcaataaa cacgcgcatt tgtggaatgt gcggcaacat
    11341 cttcggtgcc tgagcgataa tagtgacatc gacgttgccc agggtataac ccttcgcctg
    11401 aatacgacgc caggcttcgc gtagcagctc gcggctgtcg gcacctttaa atgccggatc
    11461 ggtatccggg aacagcttgc cgatatcccc cagcgccgcc gcgccaagca atgcatcggt
    11521 caacgcatgg agcgccacgt cgccatcaga atgcgccagc aatccttttt cgtaaggaat
    11581 gcgtacgcca ccaatgataa ttgggccttc accgccaaag gcatgtacgt caaaaccgtg
    11641 tccaattcgc attatgtatt ctcctgatgg atggttcggg tgaggtaaaa ctcggccagt
    11701 gccaaatctt ccgggcgcgt gactttaatg ttatccgcac ggccttcgac caactgagga
    11761 tggaatccgc aatattccag cgccgaggct tcgtcggtaa tagtcgcgcc ttcatttaga
    11821 gcgcgcgtca gacagtcatg taacagctca cgagggaaaa attgcggcgt cagcgcgtgc
    11881 cataagccgt tgcgatcaac ggtatgagca atggcatttt tgcccggttc ggcacgtttc
    11941 atcgtatcgc gcactggtgc ggctaggatc cctcccgtgc ggctggtttc gctcaacgcc
    12001 aacaatcgcg cgaggtcatc ctgatgcaga caaggacgag cggcgtcatg caccaatacc
    12061 cactgcgcgt cgccagcggc tttcaaacct gccagcacgg aatcggcacg ctcatcaccg
    12121 ccatctacaa cggtgatttg cggatgattc gccagaggaa gttgtgcaaa acggctatcg
    12181 ccaggactta tggcaatgac gacacgtttc acccggggat gcgccagcag cgcatgcacc
    12241 gagtgttcaa gaatggtttg attaccgatt gagagatatt gcttaggaca ttccgtttgc
    12301 attcgacggc caaatccggc cgccggaacc acggcgcaaa catccaaatg agtggttgcc
    12361 atgttaattc ccgggctgat ttatcgattg ttttgccccg cagactgtgc gcgcttcgac
    12421 gcgtcaggca ccagacgata aaaagtttcg cccggcctgg tcatgctgag ttcattacgc
    12481 gcacgctctt cgagcgcctc ctggccgcca ttgagatcgt caatttcggc aaaaagttga
    12541 tcgtttcgcg ctttaagttt cgcgtttgta gcttgctgtg ccgccacatc atcattgacg
    12601 cgggtatagt catgtatacc gttcttaccg aaccacagcg aatactgtag ccagaccaga
    12661 atagccagca acagcagcgt tagtttaccc atcctgcccc ctgaaaaacg gcatcatcat
    12721 cccatgcatc cgaagacgac tctacatcct ctgttgggga taccgcgaca acgcgggcaa
    12781 atgtaccaca tttgtccatt gttacgtata cccagggcgt gcagaacata atctcattat
    12841 tagttacggt ttgaattatg aacagaggag acaagaaagt acaaattagc ccagtagcca
    12901 cataaacagt gcgccaaaca taatgcctac tgtcaccagg gtgaaaacaa tactgtagcg
    12961 tagctttccg tccatcaatg aatgcagcgc aatccccacc actaccgcga cgggcatcag
    13021 cgccagaaag aaaggccagg tgtagataaa gaagaacagc gtgttagagc cataaatcaa
    13081 catcggcatc gccagcgcaa ataaccagga gataaaaccg accacggcac caggcagtga
    13141 ccatgtggtt tcttcatcct cagtaaggct gtcgttattt gttagtgtaa tgttatggct
    13201 attacgcata tttgatcctg ttactttgac gaaccgggca tggaaacccg gtggtgtctc
    13261 aggatctgat aatatcgttc tgtctcaaca gatctaataa ttgctgtacc aaatttgtta
    13321 ctaattgttc accattgaga tgaatttctg ccgattcagg cgcttcgtaa acggaatcta
    13381 ttcccgtaaa gttgcgcagt tcaccggcac gcgctttctt atataagcct tttggatcgc
    13441 gggcttcgca aatcgccagc ggcgtatcga caaacacttc gataaagcgc ccttctccta
    13501 cgcgttcacg aaccatctgg cgttcggcgc ggtgtggcga gataaatgcg gtcagcacca
    13561 ccagtccggc ttcaaccatc aaattcgcca cttcaccgac gcgacggata ttctctttac
    13621 gatcggcatc gctaaaaccg agatcgctgc ataatccgtg gcgaacatta tcgccatcca
    13681 gcagatacgt actgacgccg agtttatgta acgcctcctc cagcgccccg gcgaccgttg
    13741 atttaccgga cccggagagg ccggtaaacc acagcactac accacgatga ccgtggtgta
    13801 gctcgcgttg ttgcacagtg accggatggc tatgccagac gacgttttcg tcatgcagcg
    13861 ccattattta tcccccagca aatcgcgcgc gccccagtgc ggaaagtggc gacgaaccag
    13921 agcattcaat tccagttcga atgcactgaa ttcagatggc gcagcagttg cctggctaac
    13981 tggctcgtgc accataccgg cacccacggt cacattgctc aggcgatcga taaaaatcag
    14041 cccacccgtc accggatttt gttgataacg atctaacacc agcggctcgt caaaagtgag
    14101 atccacgagg ccgatcccat tcagtggcag gttttcaact tcacgctggg taaggttatt
    14161 aatatcaacc tgatagcgaa tgccatcaac acgcgcgcgc gtcttcttac cggcaatttt
    14221 gatgtcgtaa ctctgccctg gagaaagcgg ctgttccgcc atccatacca catccaccga
    14281 cgcgctctgc accgccggta acgcttcgtc tgccgccagc agcagatcgc cacggctgat
    14341 gtcgatctca tccgtcagca ccagggtgat cgcttctccg gcaaaggctt cttcgcgatc
    14401 accatcaaaa gtcacgatcc gcgcgacgtt tgattccaca ccagagggca gcacttttac
    14461 acgttgcccg acttccacgc gaccggatgc cagcgttccg gcgtagccac gaaaatcgag
    14521 attcgggcga ttaacgtact gcaccgggaa gcgcattggc tgagcatcca ccactcgctg
    14581 gatctccacg gtttccagca cttcgagcag tgtcggaccg ctgtaccacg gcatactttc
    14641 actttgcgat gccacgttgt cgccttccag tgcagagagc ggcacaaagc ggatatccag
    14701 attacccggc agctgcccgg caaaggtcag ataatcttca cgaatacggg tgaacgtctc
    14761 ttcactgtaa tccaccagat ccattttgtt gatcgccacg accagatgtt tgatccccaa
    14821 cagtgtggag ataaaactgt gacgacgggt ttgatcgagc acgcctttac gggcatcgat
    14881 cagtaagatc gccagttcac atgtcgatgc gccagtcgcc atattgcgag tgtactgctc
    14941 gtgccctggg gtgtcggcga taataaattt acgcttctcg gtagagaaat agcggtaggc
    15001 cacatcaata gtgatgccct gttcgcgctc agcttgcagg ccgtccacca gcagagccag
    15061 atccagcttt tcgccctggg taccgtgacg cttactgtcg ttatgcagcg atgagagctg
    15121 atcttcatag atttggcgag tatcgtgcag caggcgacca atcagagtgc ttttgccgtc
    15181 atcgacgctg ccacaggtca gaaaacgcag caggctttta tgttgttgcg caatcatcca
    15241 ggcttcgacg ccgccttcat tggcgatttg ttgtgcaagt gcggtgttca tcttaaaaat
    15301 acccctgacg ttttttcagc tccatagatc ccgcctggtc gcggtcaatc acgcggccct
    15361 gacgttcact ggtggtggaa accagcatct cttcgatgat ttccggcagt gtttgtgcat
    15421 ttgactccac cgcaccggtc agcggccagc agcccagcgt acggaaacgc accatccgtt
    15481 ttttaatcac ttcgcccggt tgcaggtcga tacggttgtc atcaatcatc atcaacatac
    15541 cgtcgcgttc cagaaccgga cgttccgcag cgagatacag cggcacaatg tcgatatttt
    15601 ccagccagat gtattgccag atatcctgct cggtccagtt agagagtggg aagacgcgga
    15661 tgctttcgcc tttgttaatt tgcccgttgt agttgtgcca cagctccggg cgctgatttt
    15721 tcggatccca gcgatggaag cggtcacgga aagagtagat tcgctcttta gcgcgggatt
    15781 tctcttcgtc acggcgcgca ccaccgaagg cggcatcaaa accgtatttg ttcagcgcct
    15841 gtttcaggcc ttcagttttc ataatatcgg tatgtttcgc gctgccgtgc acgaatggat
    15901 taatccccat cgccacgcct tccgggtttt tatgcaccag cagctcgcag ccgtaggctt
    15961 tcgccgtacg atcgcggaac tcatacatct cgcggaattt ccagccggta tcgacatgca
    16021 gcaacgggaa aggcagcgta cctggataaa acgccttgcg cgccaaatgc agcatgacgc
    16081 tggaatcttt accgatagag tagagcatca ccggatttga gaattctgcc gccacctcgc
    16141 gaataatgtg gatgctttcc gcctccagtt gccgcaggtg agtaagtcgt atttgatcca
    16201 taaccgttcc tttgcaatac cgctattttc ttgccatcag atgtttcgac tatagggagc
    16261 gtaagagaac gaatgaaatt accaattaga atgagtagtt ccttaacgga ataacgattt
    16321 ggcaaagcta atatcaaaaa gtgcttaagg caccggattt ctggcgttta ggaagatttg
    16381 aaattgtttt agcgcagcgg aagtttcata ctatggcggt aaaaaaattt gcatggtatt
    16441 taaggactca ctatgttttc cgcattgcgc caccgtaccg ctgccctggc gctcggcgta
    16501 tgctttattc tccccgtaca cgcctcgtca cctaaacctg gcgattttgc taatactcag
    16561 gcacgacata ttgctacttt ctttccggga cgcatgaccg gaactcctgc agaaatgtta
    16621 tctgccgatt atattcgcca acagtttcag caaatgggtt atcgcagtga tattcggaca
    16681 tttaatagtc ggtatattta tactgcccgc gataatcgta agagctggca taacgtgacg
    16741 ggaagtacgg tgattgccgc tcatgaaggc aaagcgccgc agcagatcat cattatggcg
    16801 catctggata cttacgcccc gctgagcgat gctgacgccg atgccaatct cggcgggctg
    16861 acgttacaag gaatggatga taacgccgca ggtttaggtg tcatgctgga actggcagaa
    16921 cgcctgaaaa atacgcctac cgagtatggt attcgatttg tggcgaccag cggcgaagag
    16981 gaaggaaaat taggcgctga gaatttactc aagcggatga gtgacaccga aaagaaaaat
    17041 acgctgctgg tgattaatct cgataactta attgttggcg ataaattgta tttcaacagc
    17101 ggtgtaaaaa cccctgaagc agtaaggaaa ttaacgcgcg acagggcgct ggcaattgcg
    17161 cgcagtcacg gaatagccgc aacgaccaat ccgggtttga ataaaaatta tccgaaaggc
    17221 actgggtgtt gtaatgacgc agaaatattc gacaaagcgg gcattgctgt actttcggtg
    17281 gaagcgacta actggaatct tgggaataag gatggttatc agcaacgcgc aaaaacagcc
    17341 gcattccctg cgggaaatag ctggcatgac gtaagactgg ataatcaaca gcatattgat
    17401 aaggcacttc ctggaagaat agaacgtcgc tgccgtgacg ttatgcggat aatgctacct
    17461 ctggtgaagg agttggcgaa ggcgtcttga tgggtttgaa aatgggagct gggtgttcta
    17521 ccgcaggggc ggggaattct aagtgatatc catcatcgca tccagtgcgc ccggtttatc
    17581 cccgctggcg cggggaactc ttactgcttg gtatgcggaa tcacaccctg aacggtttat
    17641 ccccgctggc gcggggaaca cactaagcat acataactgt ttttaaacaa atttattccg
    17701 catcaacaat ctaccaacta aattaaaaca tttccttatt tttaaagaac ggataactta
    17761 ttgattatca acaggaagaa aagaaaccaa acgtaaccca tccaaatcca ccggaatacg
    17821 tctgttttct ccccaggtct gaaattcaaa acccgactcg atattggtcg cccaggccat
    17881 caccacattt ccgcaaccag ccagttgggt aatttgctgc cagatcatct cccgaatacg
    17941 ttttgatgta tcaccaacat acacaccggc acgcacttcc agtagccaga ttgcgagccg
    18001 tccacgtaag cgcggaggga cattttctgt aacaaccacg accatgctca tccgccgcgc
    18061 ccccggtgac cactatcacc cagcgtttca ggttcaggga tggcaggcgg taacatatcc
    18121 ggcgcgggtt gtggtggttc aatttcacct gcagcaagga cttcctcaat taacggtatt
    18181 aatttgcccg ttaacttagt gctacggaaa atatcgcgac aggctaatct gacttcttta
    18241 tcaggttctg cgggttgcct cgctgctatt tcaaatgcct ttggcacaac cgaatcaaat
    18301 ttaatgatat cggctatgtc ataaacaaat gaaagcggtt tgccactatg gataaaccca
    18361 atagcgggcg catatcccgc ggctaatact gccgcttcag atataccgta cagacatgat
    18421 gtagcagcac tgatgcagcg attcacaaca tcgccttttt cccagtcttt aggatcgtat
    18481 ttgcgaccat tccatttcac accatattgt ttcgccagta atgcatacgt ctggcgaacg
    18541 cgagatccct caattccacg taactgctcc actgaacggc gagctggcgg tggttcacga
    18601 aaacgtaatt catacatttt gcgcaccacc ttcaaccgta gatcttccgt taaagccagc
    18661 tttgcctagt agagtaattt atctgcccgc gcccctccgg gttgtccgga ggagtaaacg
    18721 cgaacgcccg cttcaccgac ccagaccagc agtgttccca ccgtggcggc cagatggact
    18781 gccgcgtggg aaactcttgt tcccggttcg agcataatgc aggcgaccga tcccactgga
    18841 atatgcgtgc ggatcccggt tttgtcgatc agcacgaaag cgccgtccag tacgtcgatt
    18901 tgaccgtact ggaggaagat catggaagtg cgatctttta atgggatcgg gctcagcggt
    18961 acaaacgtca cgcctctgct ccgggtttga tcagcatcag accacaaccg aacgcccggc
    19021 ttttgccata cccctgactg agacgttgca gaaataaccc aggagcggtg accgtaagca
    19081 tccccgtata atccacgcta ctgaactgga tcagttgccg ggagttttcc cgccgcagtt
    19141 gctgttgtcg gtaggcatca accgaagtat ccagcagtgt aaaaccgctt ctctccccct
    19201 gcgctgccag ccagtccagc gcagcctgtt gttgatgcaa ccagacatca cttccttcca
    19261 cctgccccct cacctgccgt ttcgcctcca tcagcaagtc gtggcgtttg cccgctttac
    19321 aaattgttgg attcgcccgc aggttgaaac aaagggattg tccggtacgc aactctggcg
    19381 caaatggtcg gcattcgatg gtgaaaatat cactttccgc cgggcgttcc tgcgacagga
    19441 caaaaaagcg
//
LOCUS       DADMKF010000071        18843 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.072, whole genome shotgun sequence.
ACCESSION   DADMKF010000071 DADMKF010000000
VERSION     DADMKF010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18843
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.072"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(253..2124)
                     /gene="ppiD"
                     /locus_tag="JNC24_002314"
     CDS             complement(253..2124)
                     /gene="ppiD"
                     /locus_tag="JNC24_002314"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAY9159045.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLDAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGAPG
                     INSDIITVDGDRAFVLRISEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            complement(2316..2588)
                     /gene="hupB"
                     /locus_tag="JNC24_002315"
     CDS             complement(2316..2588)
                     /gene="hupB"
                     /locus_tag="JNC24_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-beta"
                     /protein_id="HAY9159046.1"
                     /translation="MNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKEGDDVAL
                     VGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            complement(2797..5151)
                     /gene="lon"
                     /locus_tag="JNC24_002316"
     CDS             complement(2797..5151)
                     /gene="lon"
                     /locus_tag="JNC24_002316"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HAY9159047.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            complement(5339..6613)
                     /gene="clpX"
                     /locus_tag="JNC24_002317"
     CDS             complement(5339..6613)
                     /gene="clpX"
                     /locus_tag="JNC24_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HAY9159048.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            complement(6739..7362)
                     /gene="clpP"
                     /locus_tag="JNC24_002318"
     CDS             complement(6739..7362)
                     /gene="clpP"
                     /locus_tag="JNC24_002318"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HAY9159049.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            complement(7608..8906)
                     /gene="tig"
                     /locus_tag="JNC24_002319"
     CDS             complement(7608..8906)
                     /gene="tig"
                     /locus_tag="JNC24_002319"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HAY9159050.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            complement(9250..9567)
                     /gene="bolA"
                     /locus_tag="JNC24_002320"
     CDS             complement(9250..9567)
                     /gene="bolA"
                     /locus_tag="JNC24_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HAY9159051.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            9872..10450
                     /locus_tag="JNC24_002321"
     CDS             9872..10450
                     /locus_tag="JNC24_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAY9159052.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            10494..11969
                     /gene="ampG"
                     /locus_tag="JNC24_002322"
     CDS             10494..11969
                     /gene="ampG"
                     /locus_tag="JNC24_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HAY9159053.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPTGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
     gene            12430..13377
                     /gene="cyoA"
                     /locus_tag="JNC24_002323"
     CDS             12430..13377
                     /gene="cyoA"
                     /locus_tag="JNC24_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit II"
                     /protein_id="HAY9159054.1"
                     /translation="MRLRKYNKSLGWLSLFAGTVLLSGCNSALLDPKGQIGLEQRSLI
                     LTAFGLMLIVVIPAILMAVGFAWKYRASNKDAKYSPNWSHSNKVEAVVWTVPILIIIF
                     LAVLTWKTTHALEPSKPLAHDEKPITIEVVSMDWKWFFIYPEQGIATVNEIAFPANTP
                     VYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHLIANEPGTYDGISASYSGPGFSGM
                     KFKAIATPDRAEFDQWVAKAKQSPNTMSDMAAFEKLAAPSEYNQVEYFSNVKPDLFAD
                     VINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHAESAH"
     gene            13399..15390
                     /gene="cyoB"
                     /locus_tag="JNC24_002324"
     CDS             13399..15390
                     /gene="cyoB"
                     /locus_tag="JNC24_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HAY9159055.1"
                     /translation="MFGKLSLDAVPFHEPIVMVTIAGIILGGLALVGLITYFGKWTYL
                     WKEWLTSVDHKRLGIMYIIVAIVMLLRGFADAIMMRSQQALASAGEAGFLPPHHYDQI
                     FTAHGVIMIFFVAMPFVIGLMNLVVPLQIGARDVAFPFLNNLSFWFTVVGVILVNVSL
                     GVGEFAQTGWLAYPPLSGIEYSPGVGVDYWIWSLQLSGIGTTLTGINFFVTILKMRAP
                     GMTMFKMPVFTWASLCANVLIIASFPILTVTVALLTLDRYLGTHFFTNDMGGNMMMYI
                     NLIWAWGHPEVYILILPVFGVFSEIAATFSRKRLFGYTSLVWATVCITVLSFIVWLHH
                     FFTMGAGANVNAFFGITTMIIAIPTGVKIFNWLFTMYQGRIVFHSAMLWTIGFIVTFS
                     VGGMTGVLLAVPGADFVLHNSLFLIAHFHNVIIGGVVFGCFAGMTYWWPKAFGFKLNE
                     TWGKRAFWFWIIGFFVAFMPLYALGFMGMTRRLSQQIDPQFHTMLMIAASGAVLIALG
                     ILCLVIQMYVSIRDRDQNRDLTGDPWGGRTLEWATSSPPPFYNFAVVPHVHERDAFWE
                     MKEKGEAYKKPDHYEEIHMPKNSGAGIVIAAFSTIFGFAMIWHIWWLAIVGFAGMIIT
                     WIVKSFDEDVDYYVPVAEIEKLENQHFDEITKAGLKNGN"
     gene            15380..15994
                     /locus_tag="JNC24_002325"
     CDS             15380..15994
                     /locus_tag="JNC24_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HAY9159056.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            15994..16323
                     /locus_tag="JNC24_002326"
     CDS             15994..16323
                     /locus_tag="JNC24_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HAY9159057.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            16335..17225
                     /gene="cyoE"
                     /locus_tag="JNC24_002327"
     CDS             16335..17225
                     /gene="cyoE"
                     /locus_tag="JNC24_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HAY9159058.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGFAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPPVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            17410..17721
                     /locus_tag="JNC24_002328"
     CDS             17410..17721
                     /locus_tag="JNC24_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HAY9159059.1"
                     /translation="MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLS
                     AFILSAAMERAESVLDNQRRRELSNQSWELMNQLIAEPAQPTLALKALMKRKNSDGRQ
                     A"
     gene            17705..18247
                     /locus_tag="JNC24_002329"
     CDS             17705..18247
                     /locus_tag="JNC24_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAY9159060.1"
                     /translation="MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRAS
                     LKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEKQRVSGVLQGSQPSEIGVVRLVM
                     LGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
                     TPNAFGAVPMFLAIQHILAA"
     gene            complement(18303..>18843)
                     /locus_tag="JNC24_002330"
     CDS             complement(18303..>18843)
                     /locus_tag="JNC24_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HAY9159061.1"
                     /translation="GIMYANGQYVEVDCKKAKEYLDKGVHIYGGPEDFLATCRKDMID
                     RKTVDDTLPVITVTRSGMRDNFLDKGFSCMDSLFATTNKLGEVANLRVTFSIRRPSGK
                     EINQTVGFAPFGLNRLNISFTDYLFGSFTSNSSLILYKPEFERKSCATVRTTIVAATA
                     TINGKDVELLKAGAIEQKW"
BASE COUNT         4215 a         4953 c         4581 g         5094 t
ORIGIN      
        1 cgccgtcggt ttcgccacag aagctgccgc cagcgcgcta tgagacattc cggcacaggc
       61 cagggacagg gtaatgagca gtgctttaat tccgtgtttc atactgtttt ctccttgttt
      121 gttaacagtg gaatcacctt aacgggagtg aaaacagtaa acaaatggca atgttcagaa
      181 atggaaaagg ccgcgaaagc gaccttttct tgttacagct gcaacgcatt tttttgcgag
      241 gcggcttcag gattattgct gttccagcgc atcgccaatt ttgattttcg cctctttacg
      301 caggttactc atcagagctt caaagacgat ttgtgcgttg ttctgggtga taccctgcac
      361 catcgctttt ttctgatctt ccggcattga accttgtttc acttcatcca gcgccagcag
      421 aaccacatta ccttgcatat cggtcgccat accgtagctc ggtttgtctt tcgctggcag
      481 tggcagtgca aacgccgcct ggctaatcgg gtcacgaccg gaacggctta aggttttcgg
      541 ctcgccaaat ttcagaccgg cagcctgcat agcttccgcc cctttgccgg ctttcaaatc
      601 aaccagcagt ttctcagcat ccactttcgc ctgttgttca gctttgttgt gctgaaccag
      661 tgccttaact tgttcctgaa catctgccaa cggtttcacc gcttccggtt tgtgctcgct
      721 gatgcgcagc acgaatgcgc ggtcgccgtc tacggtgatg atgtcagagt tgatgcccgg
      781 cgcgccgttt tcacctacca gaccgccgtt aaagatagcg tcggcaaccg gcttgaagtt
      841 caactcttcc ggcaggttat ctttgctgaa ccaacccgtc tgagtggctt taacgccggc
      901 agcttgctct gcaccggcca gagactcggt gtcgttgctt gccgcatcgc tcactttctg
      961 ctgcagcgca tagtacgcat cgagggcttt ttcgtgtttc actttcgccg caatgtcgtc
     1021 acgtacttcg tctaacgatt tcactttcgc tggctgaatg tcgtccagac gtacaatcag
     1081 gaaaccgacc gaagatttga tgacaccaga cagttggcct ttttctttca gaccagcatt
     1141 tttcagttcg tcggggatag tggcatcttc taaccaaccc atatcgccgc cgttacgggc
     1201 agagataata tcggcagatt tttctttggc taatgcagca aaatcaccgc ctttattcag
     1261 ctcatcaagt accgctttcg cttcatcttc agttttggtc tggatgatgc tgtagcgggt
     1321 acgctgcggc tgggtgaatt gatcctgatg ctgatcgtag tagctctgga tatccgcatc
     1381 gctaaccggt tgctgcatcg ttgcggcatc cagcttgatg taactcacgc ggaattgttc
     1441 cggcgtcatg aaattgtttt tgttttgttc gtagtagctg gcaatctcct gttcggtcac
     1501 aggctgcttc gccgccagcg cgttaacatc gatagtcgcc tcacgcacca cgcgttgttg
     1561 cgcgaccagt gccgccagct cgtcggtttc acctttcagc ataaaatcgg taccggcaac
     1621 accgttaatc agctgttggg tagtgagctg gttacgcagc gcctgggcgt actgatcggc
     1681 ggtcatcccc atctggttga ggataccgtt atagcggctg ttatcaaatt tgccgtcaac
     1741 ctggaaggct ggggtcgcga aaatcgcctg tttaacctgc tcatcactga tacccagttt
     1801 cagctcacga gcgtactgat ccaacagcgc ctcgtcgatc agacgattca gcacctgttg
     1861 acgcagggtt ttcatatagc cttcgttcgc tgccagctcg gagtattgat cgcccagctg
     1921 ttgctgcatg cgattacgct cgctgttaaa ggcgttctcg aactgcccac ggctgatttc
     1981 ctggtcattc acttttgcgg cgtagttatt gcctccgcca atcaggtaac cactcacgcc
     2041 ggtcaatatg aacgacacga taatgatacc gaaaataatc ttgagcacga gactgtttgc
     2101 agccgtgcgt aagctgtcca tcatggtgta acaacactcc gctgtagatg actgtgtacc
     2161 tcacgcaacg tatcgatgcg cgcaaaggcc tattgtgaca agaaacgggg gcaattgtca
     2221 gcccacaaga cacagaaagt caccgaatac aaataaaaaa ggcacatcag tagatgcgcc
     2281 cttgaacttc gtcacatccc cactggggac aacgcttagt ttaccgcgtc tttcagtgct
     2341 ttacctgcac ggaagctcgg tactttagcg gcagcgatgg tgatctcttt accggtctgc
     2401 gggttgcggc cagtacgggc agcacgctct ttaacggcaa aagtaccaaa acctaccagt
     2461 gctacatcat ccccttcttt cagagattca gttacggaag caataatagc atctaacgca
     2521 cggccagccg cagctttaga gatatcagcc cctgcagcaa tcttgtcgat caattgagat
     2581 ttattcactc ttctcttcct ctttataatt tatatcgcac ttgaatcctt caaggtacga
     2641 acgcgcagca gttatatcag gccagccatc cccttacaac accccttaat aagggcaagc
     2701 ccgatccgcc atctaactta gcgagacaaa aaaaggctgg caagcccgaa ttagcctgcc
     2761 agccctgttt ttattagtgc attttgcgcg aggtcactat tttgcagtca caacctgcat
     2821 gccagacggt tcattttgca gcgccagagt cagaacttcc tcaatgcgct tcacaggatg
     2881 aatgtccaga tcggcaatta cgttgtcagg aatctcttcc agatcgcgtt tattttcgaa
     2941 cggaattagc actgttttaa tcccgccgcg atgcgctgcc aggagttttt ctttcaaacc
     3001 accgatcggc agtacctgac cacgcagagt gatctcaccg gtcattgcca catcggcacg
     3061 aaccgggtta ccggtcaggc aagaaaccag cgcggtgcac atagcaatac cggcactcgg
     3121 accatctttc ggcgtcgcac cttccggtac gtggacgtgg atgtcacgtt tttcgtaaaa
     3181 atcagggttg atccccagtt tttccgcacg cgcacgaacc accgttaacg ccgcctgaat
     3241 agactcctgc atcacttcgc cgagcgaacc ggtataggtc agtttgcctt tacccggaac
     3301 acatgcggtt tcaatggtca gcaagtcacc gcccacttcc gtccacgcca gaccggttac
     3361 ctgaccgaca cggttttcgt tatcagcgcg accatagtcg aaacgctgaa caccgaggta
     3421 gtcatgcagg ttatcgccgt taatttcgat atgttttaat gacttatcga gcagtaactg
     3481 cttaaccgct ttgcggcaca gtttggagat ttcacgctcc agaccacgca cgcccgcctc
     3541 acgggtgtag taacgaataa tgccgataat ggcgctatcg tcgacggtca gctcaccttt
     3601 tttcagtgca ttacgttcaa tctgcttcgg cagcaggtga cgtttggcga tgttcagttt
     3661 ttcatcttcg gtataaccgg agaggcgaat cacttccata cgatccagca gcggtgccgg
     3721 aatgttcatg gagttcgacg tcgcgacaaa catcacgtcg ctgagatcgt aatccacttc
     3781 caggtagtgg tcgctgaacg ctacgttctg ctctggatcc agcacttcaa gcagtgcaga
     3841 ggccggatcg cctcgcatgt cagaagacat tttgtcgatc tcatcgagca ggaacagcgg
     3901 gtttttcacg cccactttcg ccattttctg gatcagttta cccggcatag aaccgatgta
     3961 agtacggcgg tgaccacgga tttccgcttc atcacgcacg ccgcccagcg ccatacggac
     4021 atatttacgc ccggtggctt tggcaatgga ctgaccaaga gaggttttac ctacccccgg
     4081 cggccctacc aggcagagga tcggtccctt gattttgttg acacggcttt gaaccgcaag
     4141 gtactcaagg atgcgatctt tcacgcgctc cagaccataa tggtcggtat caaggatttc
     4201 ctgcgcctga cgcaggtctt ttttgacctt gctgcgcgca ttccacggta cctgtaccat
     4261 ccagtcgata taaccacgca ctacggtcgc ttctgccgac atcggagaca tcattttcag
     4321 cttctgcaac tctgcttccg ctttctcttt tgcctctttc ggcatcttcg ccgcgtcgat
     4381 tttgcgcttc agggcttcgt tttcgtccgg cgcgtcgtcc atttcaccga gttctttctg
     4441 aatagctttc atttgctcgt tcagatagta ctcacgctgg gatttctcca tctgcttttt
     4501 aacgcggttg cgaatgcgtt tctcaacctg cagcagatcg atttccgatt ccatcattgc
     4561 catcagatat tccagacgtt cgttaacgtc ggacatctcc agaacggact gtttgtcagc
     4621 cagtttcagc ggcatatgtg cagcaatggt atccgccaga cgcgccggat cgtcgatgct
     4681 attcagcgac gtcagcactt ctggtgggat ttttttgttc agcttgatgt agccttcgaa
     4741 ctggctgatt gcagtacgca ccagcacttc ctgttcccgc tcatcaatgg tcggcgactc
     4801 cagatactcc gccttcgcag aaaagtgttc gccattgtca gagagcgcag aaatacgcgc
     4861 gcgctgtaac ccctcgacca gcactttgac ggtgccgtca ggcagtttca gcatctgcaa
     4921 tatagaggcc acggtcccga cggtgaaaag atcgtttaca cccggctcat ccgttgaagc
     4981 ttctttctgc gcgaccagca taattttttt atcatggtcc atcgccgctt ccagacaacg
     5041 gatagatttt tcccgcccga caaataaggg gatgaccatg tgcggataaa ccaccacatc
     5101 gcgcagcggc aatacgggga tttcaatgcg ttcagaacgc tcaggattca tagagctctc
     5161 tcttagttta atttccgcca ggtaatcaga tgacacgact gtgcttcacg ccatctatta
     5221 acatgtacgt cagtatatgg ggatgtttcc cccacattca acgccgagaa tagaggaaaa
     5281 attaaagggg agataaaatc cccccttttt ggttaactaa ttgtatggga atggttaatt
     5341 attcaccaga tgcctgttgc gcttccggct taccataaat cagcaacggt ttgctttgac
     5401 catcaattac cgactcgtcg ataaccactt tttcgacgtc ttccatggac ggcagatcgt
     5461 acatggtatc gagcagtgcg gcttctacga tggaacgcag gccacgggca ccggttttac
     5521 gcgccatcgc tttcttagcg atagcatcca gcgcctcgtc acggaattcc agatccacgc
     5581 cttccagatt aaacagcgcc tgatactgct tggtcagggc gtttttcggc tctttgagga
     5641 tctgaatcag agcttcttcg ctcagttcat tcaacgttgc tacaaccggc agacgaccaa
     5701 tgaactcagg gataagacca aacttgatca gatcttccgg ttcaacctgc gccagcagct
     5761 cgccttcgct tgctttgtcg gacttcgctt ttaccgtcgc gccaaaacca atgccggagc
     5821 cggtttctac acggtgggaa atcactttat ccagaccggc aaacgcaccg ccacaaataa
     5881 acaggatctt agaggtatca acctgcaaga attcctgctg cggatgttta cgcccacctt
     5941 gcggtggaac agcagctacc gtaccttcga tcagtttcaa cagtgcctgc tgtacgcctt
     6001 caccggaaac gtctcgggta atggacgggt tgtctgactt acgagaaatc ttgtcgattt
     6061 catcgatgta gacaatcccg cgctgcgctt tctgtacgtc gtaatcgcac ttttgcaaca
     6121 gcttctgaat gatgttttca acgtcttcgc ccacataacc ggcttcggtc agcgtggttg
     6181 cgtcggccat ggtgaacggg acgtccagca ggcgcgccag cgtttcagcc agcagcgttt
     6241 taccggaacc ggtcggaccg atcagcagaa tgttactttt gcccaactcg acaccattgc
     6301 tggtatcgcc gttgcgcaga cgtttgtaat ggttgtatac cgcgaccgcc agcacttttt
     6361 tcgcctgttc ctggccgata acgtaatcgt ccaggtggtt gcgaatttca tgcggcgtcg
     6421 gtagcgcact gcgttcacga tgcggtgcaa cttctttaat ctcttcgcga atgatgtcgt
     6481 tacataaatc aacacattcg tcgcagatat acacggatgg accggcaatc agcttgcgca
     6541 cttcatgctg gcttttgccg caaaaagagc aatacagcaa tttgcctgag ccatctttgc
     6601 gtttatctgt catgggtcaa aacctcttct ttgttctttg tgccgcacac gacgacgcaa
     6661 atgccattct caggcgcaag ccgcttacag cgttgtgccg ccctggataa gtatagcggc
     6721 acagttgcgc ctctggcatc aattacgatg ggtcagaatc gaatcgacca gaccgtattc
     6781 caccgcttca ggggcggaaa ggaagcgatc gcgctcggta tcacgttcaa tctgttctaa
     6841 tgattgaccc gtatgaagcg ccataagttc attcatgcgc cctttaactt tcagaatttc
     6901 acgggcatga atttcgatat cggtcgcctg gccctggtag ccgcccaacg gttggtgaat
     6961 catcacgcgc gaattcggca ggcaaaaacg tttacctttt gcccctgcgg tcagcaagaa
     7021 agcgcccatc gaggccgcct ggcccataca gatggtgctg acatcaggct tgataaactg
     7081 catggtgtca tagatagaca tcccggcagt gatcaccccg cctggggagt taatgtacag
     7141 atagatatct ttttctgggt tttccgcttc taggaacagc atctgcgcca caatcaggtt
     7201 cgccatgtgg tcttcaacct ggccagtcag aaaaatgacg cgttccttaa gtagacgaga
     7261 atagatatca aaagagcgct caccgcgtga ggtctgttca atgaccatcg gcaccagcgc
     7321 catatggggt gcaaagttat ctcgttcgcc gctgtatgac atttccgtct cctggataaa
     7381 attgaaaaaa cctgctgtac cgattgtaac cttatggacg gattatcagc tagtccctgt
     7441 gttatgggta cggcatcacc atatttcaag cataacaatc ttttgttgtt acgctaacac
     7501 cgaaagaggc ttttcgcacg gttccatgca aaattcatga caaaaaaaag cccaccacct
     7561 ggcggtgacg ggcctttgtg cgaatttagc gcgttatgct gcgtaaatta cgcctgctgg
     7621 ttcatcagct cgttgaaagt ggtttctttt tcagtcactt tcgctttcgc cagtacagct
     7681 tcaacagcct gttcttccag agcaacattg cgcatgttgt ccatcagttc tttgtttttg
     7741 ctgtagaact cgataacttc tttcggatct tcgtacgcag aagccatctc ttcgatcagg
     7801 cctttcacgc gctcttcgtc agctttcagt tcgttggtgc ggataacttc gcccagcagc
     7861 aggccaacaa ctacgcggcg tttagcctgt tcttcgaaca gttcgcgcgg cagttccaga
     7921 gcttgttttt cgttgccacc gaaacgctgt gcagcctggc gacgcagaac gtcgatttcg
     7981 ctgtcgatca gcgcagccgg tacgtcgatg tcgttagctt ttaccagacc ttcgatcgcc
     8041 tgagacttaa cgcggttacg gatggcgctc ttcagctcgc gctccatgtt tttacgcact
     8101 tcagcgcgca gaccttctac ggaaccatct tcaacgccga aacgtttgat gaattctgca
     8161 gtcagttccg gcagttcacg ctcttcaact ttcttcaggt tgatagcgaa tttcgctgct
     8221 ttacctttca gattttctgc gtggtattct tccgggaagg tcacgtcgat ggtgaactct
     8281 tcgccagctt tgtggccttt gataccgtct tcaaagcccg ggatcatacg accctggccc
     8341 atcgccagta cgaaatcaga cgctttaccg ccttcgaact cttcgccgtc tacagaaccg
     8401 gtgaagtcga tggttacgcg gtcttctgct tcaacggcgc cgtctttttc tttccaggtc
     8461 gcctgctgtt tacgcagagt atccagcatg ccgtcaacgt cagcgtcggt cacttcaacg
     8521 atcggttttt caacttcgat cgcttccaga ccctgcagtt caacttccgg ataaacttca
     8581 aactctacag agtaggtgaa gtcttcaccc agcttgtatt cgcccggaac ataagtcggt
     8641 gcgccagccg gattgatttt ttctttaatg atggcgtcaa tgaagttacg gctcatcagg
     8701 tcacccagaa cgtcctggcg tacagacgcg ccataacgct gagcaacgat attcattggc
     8761 actttgcctt tgcggaagcc gtcaatacgt acttttttcg caacgttgac cagctcgctt
     8821 ttaacagcgg tctcgatgct gtcagcagcg atagtaatcg ttacacggcg gccaaggcct
     8881 tgagtggttt caactgaaac ttgcatcttg ttacctcaaa aaatcacagt gctcggtcaa
     8941 ctcttacccc gcaagcacag gttgttggct tcgcggaacc gggatgttct tataatcaat
     9001 cacattccct gttaccagaa tcatcccgaa agcattcaaa taggacgcgg cattatagcg
     9061 gcatcacttt tatgagtcga gaacggttat tgcgcgctgt cgcgtatttt ttcacaattc
     9121 ccgggcaatt ttcgtctgaa tgctgggcaa aaagcgcaaa atccagacaa aacaaaacgg
     9181 cccgcaggcc gtttttcata aaatctttag caacatactg gaaaagcgcc gacagttgca
     9241 aatgcgtttt tacgcgatgc ttcctgctcc acgacaggga ggagaggcaa agacggtgtc
     9301 ctgcaaccct tcccactcct taatagtgta agtatgcaga gccagcgcat gaacggtagt
     9361 agagagttcc tccgctaaag tactgtaaat cattcgatga cgattcagaa aacgttcacc
     9421 cgtaaaacga tcgctgacca gcacaacttt aaaatggctt tcagagccgg ctgggacatt
     9481 gtgacgatag ctttcatcca ctacttcgag gaatacgggt tggaacgccg cccttaattt
     9541 ttcttctatc cgctcacgta tcatcatgaa atatctcctc cgacaacgct gagatgtcat
     9601 ccatcccttt aaatactagc cgcttttacc gtttccattg cagcttaaca acaaatattt
     9661 aggtttcgtc ctggtttttc cgtcaagcgt atgaagttta ctgatttaaa gattgaaaca
     9721 caggcctcgc atcagcgttt cttttaccag gggtgattat ttcgccaaca tgatgaattt
     9781 acatgcctta cccacttccc ctcgccgttg gcaatgttat gatggcggga atttttacca
     9841 aacacgctta agagtctctg agagcccgaa catgttcaaa aaaatcctct tcccgttagt
     9901 tgctctgttt atgcttgcag gatgcgcaaa accgccaaca actattgaag tttccccgac
     9961 gattacactg ccacagcagg atccaagcct gatgggcgtc accgtaagca ttaatggtgc
    10021 cgatcagcgt accgatcagg cgctggcaaa agtcacccgc gataatcaaa tcgttaccct
    10081 gaccgcctcc cgagatctgc gtttcctgct gcaagaagtg ctggaaaaac agatgaccgc
    10141 gcgtggttac atggttggtc cgaatggccc ggttaatctg caaatcatcg ttagccaact
    10201 gtatgctgac gtgtcccagg gcaacgtgcg ctacaacatc gcgaccaaag cggatatcgc
    10261 catcatcgct accgcgcaga atggcaacaa aatgaccaaa aactatcgtg ccagctacaa
    10321 cgttgaaggt gcgttccagg cctccaacaa aaatatcgcc gatgcggtta acagtgtgct
    10381 gaccgatacc atcgctgata tgtctcagga caccagcatc cacgaattca tcaagcagaa
    10441 cgcgcgttaa tttctgccct ctggcccggt gcaaaccggg cctgtagacg cccatgtcca
    10501 gtcaatattt acgtattttt caacagccgc gttcagccat attgctgatc ctgggttttg
    10561 cttccgggct accgctcgcc ctgacatccg gtaccttaca ggcctggatg acggtcgaga
    10621 atattgatct caaaaccatt ggtttcttct ctctggtagg ccaggcttac gtttttaaat
    10681 tcctctggtc accgctgatg gaccgctaca cgcccccatt ttttgggcgg cggcgcggtt
    10741 ggctgctcgc cacgcaaatc ctgttattag tcgccattgc ggcgatgggt tttctcgaac
    10801 ctggcaccca actccgttgg atggcggcac tggcggtggt gatcgctttt tgctcggcct
    10861 cccaggatat tgtcttcgat gcgtggaaaa ccgatgtgct cccggcagaa gaacgtggtg
    10921 cgggcgcggc aatcagcgtg ctgggttacc gtttagggat gctggtttcc ggcggcttag
    10981 ccctgtggct ggcagataaa tggctgggct ggcagggcat gtactggttg atggcggcgc
    11041 tgttgatccc ttgtattatc gcgacgttgc ttgcaccaga accgaccgac accattcctg
    11101 tgccaaaaac gctggaacaa gcggttgttg cacctctgcg agatttcttt ggtcgcaata
    11161 atgcctggct tattttgctt cttatcgtgc tgtataagct gggcgacgca ttcgccatga
    11221 gcctgacaac cacgtttttg attcgcggcg tcgggtttga tgcgggtgaa gtaggcgtgg
    11281 ttaacaaaac gcttggctta ctggcgacca ttgtgggcgc attgtacggt gggattttga
    11341 tgcagcgcct gtcgctgttc cgggcgctgc tgattttcgg cattttacaa ggtgcgtcta
    11401 acgctggtta ctggctgctg tcgattactg ataagcatct ctacagcatg ggcgcagccg
    11461 tctttttcga aaacctctgt ggcgggatgg gcacatcagc ctttgtcgca ctgttaatga
    11521 cgctgtgtaa taagtcattt tccgctactc aatttgccct gctctcagcg ctttccgctg
    11581 taggacgagt ttatgtcggc cccgtggcgg gttggtttgt tgaagcacac ggctggtcga
    11641 cattctatct attctccgtc gccgctgccg taccggggct tattttgctg ctggtttgcc
    11701 gccagacgct tgaatataca cgagtaaatg acaactttat ctcccgtacc gaatatccga
    11761 caggttatgc ctttgccatg tggacactgg cggcgggcgt cagcctgttg gccgtgtggt
    11821 tactgctgtt gacgatggac gcgctggatt tgacgcactt ctctttcctg cctgctctgc
    11881 tggaagtcgg ggttttagtc gccctttctg gcgtcgtgct tggtggtttg ctggattatc
    11941 tggctctacg aaaaacgcat ctgacgtaat ctgtaaatat tatttagctg tagtaatcac
    12001 tcgccgtaat tattacggct aaataaacat cagtatcgct taataatatt tgttcttttg
    12061 tgcggcttag cgtttggaaa ttattgacgc aatttatgaa ttctattttt tcttacacga
    12121 ttcagctaat gagtctttat tttcccccca cccaattgtc accataatga taattatttg
    12181 ttaaacaatt gttttattca agattggtta taccaattgc ccgtccaaac tccttctgca
    12241 ctcccctttc gttataacgc ccttttgcaa cagcttctta aaatcaactt gatatgtttt
    12301 gcaacatatg taacctgaca gccaaatcca agtaacagga atttaatcat gtttacagta
    12361 atgtaacctc cccgtaaaat gcccacacac tttaaacgcc accagatccc gtggaattga
    12421 ggtcgttaaa tgagactcag gaaatacaat aaaagtttgg gatggttgtc attatttgca
    12481 ggcactgtat tgctcagtgg ctgtaattct gcgctgttag atcccaaagg acagattggt
    12541 ctggagcaac gttcactgat actgacggca tttggcctga tgttgattgt cgttattccc
    12601 gcaatcttga tggctgttgg tttcgcctgg aagtatcgtg cgagcaataa agatgctaag
    12661 tacagcccga actggtcaca ctccaataaa gtggaagctg tggtctggac ggtacctatc
    12721 ttaatcatca tcttccttgc ggtactgacc tggaaaacca ctcacgctct tgagcctagc
    12781 aagccgctgg cacacgacga gaagcccatt accatcgaag tggtttccat ggactggaaa
    12841 tggttcttca tctacccgga acagggcatt gcaaccgtga atgaaatcgc tttcccggcg
    12901 aacactccgg tgtacttcaa agtgacctcc aactccgtga tgaactcctt cttcattccg
    12961 cgtctgggta gccagattta tgccatggcc ggtatgcaga ctcgcctgca tctgatcgcc
    13021 aacgaacccg gtacttatga cggtatctcc gccagctaca gcgggccggg cttctcaggc
    13081 atgaagttca aagctattgc aacaccggat cgcgccgaat tcgaccaatg ggtcgcaaaa
    13141 gcgaaacagt cgccgaacac catgtctgac atggctgcgt tcgaaaaact ggccgcgcct
    13201 agcgaataca accaggtgga atatttctcc aacgtgaaac cagacttgtt tgccgatgtg
    13261 attaacaagt ttatggctca cggtaagagc atggacatga cccagccaga aggtgagcac
    13321 agtgcacacg aaggtatgga aggcatggac atgagccacg cggaatccgc ccattaaagg
    13381 ggttgaggaa gaataaagat gttcggaaaa ttatcacttg atgcagtccc gttccatgaa
    13441 cctatcgtca tggttacgat cgctggcatt attttgggag gtctggcgct cgttggcctg
    13501 atcacttact tcggtaagtg gacctacctg tggaaagagt ggctgacctc cgtcgaccat
    13561 aaacgcctcg gtatcatgta tatcatcgtg gcgattgtga tgttgctgcg tggttttgct
    13621 gacgccatta tgatgcgtag ccagcaggct cttgcctcgg cgggcgaagc gggcttcctg
    13681 ccacctcacc actacgatca gatcttcacc gcgcacggcg tgattatgat cttcttcgtg
    13741 gcgatgcctt tcgttatcgg tctgatgaac ctggtggttc cgctgcagat cggcgcgcgt
    13801 gacgttgcgt tcccgttcct caacaactta agcttctggt ttactgttgt tggtgtgatt
    13861 ctggttaacg tttctctcgg cgtgggcgaa tttgcgcaga ccggctggct ggcctatcca
    13921 ccgctatcgg gaatagagta cagtccggga gtcggtgtcg attactggat atggagtctc
    13981 cagctatccg gtataggtac gacgcttacc ggtatcaact tcttcgttac cattctgaag
    14041 atgcgcgcac cgggcatgac catgttcaag atgccagtat ttacctgggc atcactgtgc
    14101 gcgaacgtac tgattattgc ttccttccca attctgacgg ttaccgtcgc gttgttgacc
    14161 ctggatcgct atctgggcac ccatttcttt accaacgata tgggtggcaa catgatgatg
    14221 tacatcaacc tgatttgggc ctggggccac ccggaagttt acatcctgat cctgcctgtt
    14281 ttcggtgtgt tctccgaaat tgcggcaacc ttctcgcgta aacgtctgtt tggttatacc
    14341 tcgctggtat gggcaaccgt ctgtatcacc gtgctgtcgt tcatcgtttg gctgcaccac
    14401 ttctttacga tgggtgcggg cgcgaacgta aacgccttct ttggtatcac cacaatgatt
    14461 atcgccatcc cgaccggggt gaagatcttc aactggctgt tcaccatgta tcagggccgc
    14521 atcgtgttcc attctgcgat gctgtggacc atcggtttta tcgtcacttt ctcggtgggc
    14581 gggatgactg gcgtgctgct ggcagtaccg ggcgcggact tcgttctgca taacagcctg
    14641 ttcctgattg cgcacttcca taacgttatc atcggcggcg tggtcttcgg ctgcttcgca
    14701 gggatgacct actggtggcc taaagcgttc ggtttcaaac tgaatgaaac ctggggtaaa
    14761 cgcgcgttct ggttctggat catcggcttc ttcgttgcct ttatgccgct gtatgcgctg
    14821 ggcttcatgg gcatgacccg tcgtttgagc cagcagattg acccgcagtt ccacaccatg
    14881 ctgatgattg cagctagcgg tgcagtactg attgcgctgg gtattctctg cctcgttatt
    14941 cagatgtacg tttctattcg cgaccgcgac cagaaccgtg acctgactgg cgacccgtgg
    15001 ggtggccgta cgctggagtg ggcaacctct tccccgcctc cgttctataa ctttgccgtt
    15061 gtgccgcacg ttcacgagcg tgatgcattc tgggaaatga aagagaaagg cgaagcgtat
    15121 aaaaagcctg accactatga agaaattcat atgccgaaaa acagcggtgc aggtatcgtc
    15181 attgcagctt tctccaccat cttcggtttc gccatgatct ggcatatctg gtggctggcg
    15241 attgttggct tcgcaggcat gatcatcacc tggatcgtga aaagcttcga cgaggacgtg
    15301 gattactacg tgccggtggc agaaatcgaa aaactggaaa accagcattt cgatgagatt
    15361 actaaggcag ggctgaaaaa tggcaactga tactttgacg cacgcgactg cccacgcgca
    15421 cgaacacggg caccacgatg caggcggaac caaaatcttc ggattttgga tctacctgat
    15481 gagcgactgc attctgttct caatcttgtt tgctacctat gccgttctgg tgaacggcac
    15541 cgcaggcggc ccgacaggta aggacatttt cgaactgccg ttcgttctgg ttgaaacttt
    15601 cttgctgttg ttcagctcca tcacctacgg catggcggct atcgccatgt acaaaaacaa
    15661 caaaagccag gttatctcct ggctggcgtt gacctggttg tttggtgccg gatttatcgg
    15721 gatggaaatc tacgaattcc atcacctgat tgttaacggc atgggtccgg atcgcagcgg
    15781 cttcctgtca gcgttctttg cgctggtcgg cacgcacggt ctgcacgtca cttccggtct
    15841 tatctggatg gcggtgctga tggtacaaat cgctcgtcgc ggcctgacca gcactaaccg
    15901 tacccgcatc atgtgcctga gcctgttctg gcacttcctg gatgtggttt ggatctgtgt
    15961 gttcactgtt gtttatctga tgggggcgat gtaatgagtc attctaccga tcacagcggc
    16021 gcgtcccatg gcagcgtaaa aacctacatg acaggcttta tcctgtcgat cattctgacg
    16081 gtgattccgt tctggatggt gatgacagga gctgcctctc cggccgtaat tctgggaaca
    16141 atcctggcaa tggcagtggt acaggttctg gtgcatctgg tgtgcttcct gcacatgaat
    16201 accaaatcag atgaaggctg gaacatgacg gcgtttgtct tcaccgtgct aatcatcgct
    16261 atcctggttg taggctccat ctggattatg tggaacctca actacaacat gatgatgcac
    16321 taagagcggc ggttatgatg tttaagcaat acctgcaagt aacgaaacca ggcatcatct
    16381 ttggcaacct gatctcggtg attgggggat tcctgctggc ctcaaagggc agcattgatt
    16441 atcccctgtt tatctacacg ctggttgggg tgtcactggt tgtggcgtcg ggttgtgtgt
    16501 ttaacaacta catcgacagg gatatcgaca gaaagatgga aaggacgaag aatcgggtgc
    16561 tggttaaagg cctgatctct cctgctgtct cgctggtgta cgccaccttg ctaggctttg
    16621 ctggctttat gctgctgtgg tttggcgcga atccgctggc ctgctggctg ggggtgatgg
    16681 gctttgtggt ttatgtcggc gtttatagcc tgtacatgaa acgccactcg gtctacggca
    16741 cattgattgg ttcgctctcc ggcgctgcgc cgccggtgat tggctactgt gcagtaaccg
    16801 gcgagttcga tagcggcgca gcgatcctgc tggctatctt cagcctgtgg cagatgcctc
    16861 actcctatgc catcgccatt ttccgcttta aggattatca ggcggcaaac attccggtat
    16921 tgccagtggt aaaaggcatt tcggtggcga agaatcacat cacgctgtat atcatcgcct
    16981 ttgccgttgc cacgctgatg ctctctcttg gcggttacgc tgggtataaa tatctggtgg
    17041 tcgccgcggc ggtgagcgtc tggtggttag gtatggccct gcgcggttat aaagttgctg
    17101 atgacaggat ctgggcgcgc aagctgttcg gcttctctat catcgccatc acagccctct
    17161 cggtgatgat gtccgttgat tttatggtgc cggactcgca tacgctgctg gctgctgttt
    17221 ggtaacacaa cctctctatt aaaaaggtgc tacggcacct tttttattgt ctgagatcag
    17281 agatacggct aataaccgta tgtcttgaca gagtgagcag atgaacataa attaatcata
    17341 cggttgatag ccgtattatg tatgagatcg actccctacg atttcattca gttaatcaaa
    17401 gaggtcataa tgcctggaaa aacagccact ctacccgatg tcgataaaac gctgaaaaat
    17461 gcgcgggtcg aactcaaaac tagtcccgac gccaaaaaca aacttcgtga agctgcgcaa
    17521 gccgttgggg tagatttaag cgcttttatt ttgagcgctg ctatggaacg cgccgaaagt
    17581 gtgcttgata accaacgtcg ccgtgagctt tcgaatcaaa gctgggaact gatgaaccaa
    17641 ctcatcgctg aacctgctca accgacgctc gccctcaagg cgttaatgaa aaggaaaaac
    17701 agcgatggtc gacaagcatg aagagattac tctgcccata gtcctctcct gtaattatca
    17761 gtctgatatt acttatcctg ggcaaaaaca gtttgattgc ggtaaccctg ttatcgataa
    17821 atttgtacgc gcatcgctaa agaaaagtgt gcgtaatagc gactgtgcgg ctaaagcact
    17881 tattgacaga caaagtggtg aactgatcgg catctgtact tttacggcat attcgctgga
    17941 aaaacaacgc gtttctggcg tccttcaggg ttcacaacct tcagaaattg gtgttgtcag
    18001 attagtcatg ttgggggtag cacggaagta tcaaaagcgg ggctttggtc aggacctact
    18061 atgtgatttt tttgaacatg taaaaataat tcaccaggca ttaccaatta aaggggttta
    18121 tcttgatgct gaccctgccg ccattaattt ttatgctcgt ctcggctttg ttcagctttc
    18181 agcgacacca aatgcttttg gtgctgtacc tatgtttttg gcgattcagc atattctcgc
    18241 ggcttagatt attatcgcca cccgccaggg caagttttgc cttagccggg ggctgagcag
    18301 cgttaccact tttgttcaat tgcccccgct ttcagcaact ccacatcctt gccgttaatg
    18361 gttgccgttg cggcaacgat tgtggtcctt acagttgcac aagattttcg ctcaaattcc
    18421 ggtttataaa gaataagaga cgaattactg gtaaatgaac caaataggta atcagtaaag
    18481 ctaatattca gccggtttaa accgaaaggc gcgaagccaa ccgtttggtt aatctcctta
    18541 ccggatgggc gacgaatgct gaatgtcacg cgaagattgg ccacttcgcc taatttattg
    18601 gtggtggcga agaggctatc catgcaggaa aaacctttat ctaaaaaatt atctcgcatc
    18661 ccagaacggg taaccgtaat cacgggtaac gtatcgtcaa ccgtttttct gtcaatcatg
    18721 tcttttcgac aagtagccag aaagtcttcc gggccaccat aaatgtggac acctttatca
    18781 agatattcct tcgctttctt acaatctacc tcaacatact gaccattggc gtacataatg
    18841 ccc
//
LOCUS       DADMKF010000072        18523 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.073, whole genome shotgun sequence.
ACCESSION   DADMKF010000072 DADMKF010000000
VERSION     DADMKF010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18523
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.073"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            <1..1710
                     /locus_tag="JNC24_002331"
     CDS             <1..1710
                     /locus_tag="JNC24_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311138.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HAY9159062.1"
                     /translation="EMRQTLTLHKGANYISLAQNIQQSGLLSAELQQNGQVQDSISTK
                     LSFVDNSWPVEQQKNVMLGGGENALMLPEQASNIRLQSSETPQEIFRNNLDALVDGPW
                     GGVINTGSRLIPLSLAWRSLADHQSAAANDIRQMIQDNRLRLMQLAGPGARFTWWGED
                     GNGDAFLTAWAWYADWQASQALGVTQQPEYWQHMLDSYAEQADNMPLLHRALVLAWAQ
                     EMNLPCKTLLKGLDEAIARRGTKDEDFSEEDIRDINDSLILDTPESPLADAVANVLTM
                     TLLKKAQLKSTVMPQVQQYAWDKAANSNQPLAHTVVLLNSGGDATQTAAILSGLTAEQ
                     STIERALAMNWLAKYMATMPPVVLPAPAGAWAKHKLTGGGEDWRWVGQGVPDILSFGD
                     ELSPQNVQVRWREPAKTAQQSNIPVTVERQLYRLIPGEEEMSFTLQPVTSNEIDSDAL
                     YLDEITLTSEQDAVLRYGQVEVPLPPGADVERTTWGISVNKPNAAKQQGQLLEKARNE
                     MGELAYMVPVKELTGTVTFRHLLRFSQKGQFVLPPARYVRSYAPAQQSVAAGSEWTGM
                     QVK"
     gene            1711..3360
                     /locus_tag="JNC24_002332"
     CDS             1711..3360
                     /locus_tag="JNC24_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2300 domain-containing protein"
                     /protein_id="HAY9159063.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLVLRGAQHDQLYQLSSSGV
                     TKVSALPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPGVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QDLIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            3365..4141
                     /locus_tag="JNC24_002333"
     CDS             3365..4141
                     /locus_tag="JNC24_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HAY9159064.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTSGIGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            complement(4215..5399)
                     /locus_tag="JNC24_002334"
     CDS             complement(4215..5399)
                     /locus_tag="JNC24_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HAY9159065.1"
                     /translation="MKNCVIVSAVRTAIGSFNGSLASTSAIDLGATVIKAAIERAKID
                     SLHIDEVIMGNVLQAGLGQNPARQALLKSGLAETVCGFTVNKVCGSGLKSVALAAQAI
                     QAGQAKSIVAGGMENMSLAPYLLDAKARSGYRLGDGQVYDVILRDGLMCATHGYHMGI
                     TAENVAKEYGITREMQDELALHSQRKAAAAIESGAFTAEIVPVNVVTRKKTFVFSQDE
                     FPKANSTAEALGALRPAFDKAGTVTAGNASGINDGAAALVIMEESAALAAGLTPLARI
                     KSYASGGVPPALMGMGPVPATQKALQLAGLQLADIDLIEANEAFAAQFLAVGKTLGFD
                     SEKVNVNGGAIALGHPIGASGARILVTLLHAMQARDKTLGLATLCIGGGQGIAMVIER
                     LN"
     gene            complement(5430..6752)
                     /locus_tag="JNC24_002335"
     CDS             complement(5430..6752)
                     /locus_tag="JNC24_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00366 family protein"
                     /protein_id="HAY9159066.1"
                     /translation="MIGRISRFMTRFVSRWLPDPLIFAMLLTLLTFVIALWLTPQTPI
                     SMVKMWGDGFWNLLAFGMQMALIIVTGHALASSAPVKSLLRTAASAAKTPVQGVMLVT
                     FFGSVACVINWGFGLVVGAMFAREVARRVPGSDYPLLIACAYIGFLTWGGGFSGSMPL
                     LAATPGNPVEHIAGLIPVGDTLFSGFNIFITVTLIVVMPFITRMMMPKPSDVVSIDPE
                     LLMEEADFQKQLPKDAPPSERLEESRILTLIIGALGIAYLAMYFSEHGFNITINTVNL
                     MFMIAGLLLHKTPMAYMRAISAAARSTAGILVQFPFYAGIQLMMEHSGLGGLITEFFI
                     NVANKDTFPVMTFFSSALINFAVPSGGGHWVIQGPFVIPAAQALGADLGKSVMAIAYG
                     EQWMNMEQPFWALPALAIAGLGVRDIMGYCITALLFSGVIFVIGLTLF"
     gene            complement(6749..7399)
                     /locus_tag="JNC24_002336"
     CDS             complement(6749..7399)
                     /locus_tag="JNC24_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit B"
                     /protein_id="HAY9159067.1"
                     /translation="MDAKQRIARRVAQELRDGDIVNLGIGLPTMVANYLPEGIHITLQ
                     SENGFLGLGPVTTAHPDLVNAGGQPCGVLPGAAMFDSAMSFALIRGGHIDACVLGGLQ
                     VDEEANLANWVVPGKMVPGMGGAMDLVTGSRKVIIAMEHCAKDGSAKILRSCTMPLTA
                     QHAVHMLVTELAVFRFIDGKMWLTEIADGCDLATVRAKTEARFEVAADLNTQRGDL"
     gene            complement(7399..8061)
                     /gene="atoD"
                     /locus_tag="JNC24_002337"
     CDS             complement(7399..8061)
                     /gene="atoD"
                     /locus_tag="JNC24_002337"
                     /EC_number="2.8.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate CoA-transferase subunit alpha"
                     /protein_id="HAY9159068.1"
                     /translation="MKTKLMTLQDATGFFRDGMTIMVGGFMGIGTPSRLVEALLESGV
                     RDLTLIANDTAFVDTGIGPLIVNGRVRKVIASHIGTNPETGRRMISGEMDVVLVPQGT
                     LIEQIRCGGAGLGGFLTPTGVGTVVEEGKQTLTLDGKTWLLERPLRADLALIRAHRCD
                     TLGNLTYQLSARNFNPLIALAADITLVEPDELVETGELQPDHIVTPGAVIDHIIVSQE
                     SK"
     gene            complement(8257..9642)
                     /gene="atoC"
                     /locus_tag="JNC24_002338"
     CDS             complement(8257..9642)
                     /gene="atoC"
                     /locus_tag="JNC24_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate metabolism transcriptional
                     regulator AtoC"
                     /protein_id="HAY9159069.1"
                     /translation="MTAINRILIVDDEDNVRRMLSTAFALQGFETHCANNGRTALHLF
                     ADIHPDVVLMDIRMPEMDGIKALKEMRSHETRTPVILMTAYAEVETAVEALRCGAFDY
                     VIKPFDLDELNLIVQRALQLQSMKKEIRHLHQALSASWQWGHILTNSPAMMDICKDTA
                     KIALSQASVLISGESGTGKELIARAIHYNSRRAKGPFIKVNCAALPESLLESELFGHE
                     KGAFTGAQTLRQGLFERANEGTLLLDEIGEMPLVLQAKLLRILQEREFERIGGHQTIK
                     VDIRIIAATNRDLQAMVKEGTFREDLFYRLNVIHLILPPLRDRREDISLLANHFLQKF
                     SSENQRDIIDIDPMAMSLLTAWSWPGNIRELSNVIERAVVMNSGPIIFSEDLPPQIRQ
                     PVCNAGEVKTASVGERNLKEEIKRVEKRIIMEVLEQQEGNRTRTALMLGISRRALMYK
                     LQEYGIDPADV"
     gene            complement(9639..11465)
                     /gene="atoS"
                     /locus_tag="JNC24_002339"
     CDS             complement(9639..11465)
                     /gene="atoS"
                     /locus_tag="JNC24_002339"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     AtoS"
                     /protein_id="HAY9159070.1"
                     /translation="MHYMKWIYPRRLRNQMILMAILMVIVPTLTIGYIVETEGRSAVL
                     SEKEKKLSAVVNLLNQALGDRYDLYIDLPREERIRALNAELAPITENITHAFPGIGAG
                     YYNKMLDAIITYAPSALYQNNVGVTIAADHPGREVMRTNTPLVYSGRQVRGDILNSML
                     PIERNGEILGYIWANELTEDIRRQAWKMDVRIIIVLTADLLISLLLIVLFSRRLSANI
                     DIITDGLSTLAQNIPTRLPQLPGEMGQISQSVNNLAQALRETRTLNDLIIENAADGVI
                     AIDRQGDVTTMNPAAEVITGYQRHELVGQPYSMLFDNTQFYSPVLDTLEHGTEHVALE
                     ISFPGRDRTIELSVTTSRIHNTHGEMIGALVIFSDLTARKETQRRMAQAERLATLGEL
                     MAGVAHEVRNPLTAIRGYVQILRQQTSDPIHQEYLSVVLKEIDSINKVIQQLLEFSRP
                     RHSQWQQVSLNALVEETLVLVQTAGVQARVDFISELDNELSPINADRELLKQVLLNIL
                     INAVQAISARGKIRIQTWQYSDSQQAISIEDNGCGIDLSLQKKIFDPFFTTKASGTGL
                     GLALSQRIINAHQGDIRVASLPGYGATFTLILPINPQGNQTV"
     gene            11632..14481
                     /gene="rcsC"
                     /locus_tag="JNC24_002340"
     CDS             11632..14481
                     /gene="rcsC"
                     /locus_tag="JNC24_002340"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HAY9159071.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRES"
     gene            complement(14681..15331)
                     /gene="rcsB"
                     /locus_tag="JNC24_002341"
     CDS             complement(14681..15331)
                     /gene="rcsB"
                     /locus_tag="JNC24_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsB"
                     /protein_id="HAY9159072.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(15348..18020)
                     /gene="rcsD"
                     /locus_tag="JNC24_002342"
     CDS             complement(15348..18020)
                     /gene="rcsD"
                     /locus_tag="JNC24_002342"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HAY9159073.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTWQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDTTATGDNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESLPNTAVNNELRILRAINEKIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVDE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAAGPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRQLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
BASE COUNT         4526 a         4535 c         4867 g         4595 t
ORIGIN      
        1 gagatgcgcc agacgctgac gctgcacaaa ggggcgaatt atatttcgct ggcgcagaac
       61 attcagcaat ctggcttgtt aagcgcagaa ctgcaacaaa atgggcaagt gcaggacagc
      121 attagcacaa aactgtcttt tgtggataac agctggcccg ttgaacagca gaaaaatgtc
      181 atgctcggcg gtggcgagaa cgcgctgatg ttgcccgagc aggctagcaa tatccggcta
      241 caaagtagcg aaacgccgca ggagattttc cgcaacaatc ttgatgcgtt agtcgatgga
      301 ccatggggtg gcgtgatcaa caccggtagc cgtctgatcc cgctcagtct cgcctggcgt
      361 tcgcttgccg atcatcaaag tgccgccgct aacgacattc gtcagatgat tcaggataac
      421 cgtctgcggc tgatgcaact ggcggggccc ggagcgcgct ttacctggtg gggtgaagat
      481 ggcaatggtg acgccttcct tacggcatgg gcatggtacg ccgactggca ggccagccag
      541 gcgctcggcg taacgcaaca accggaatac tggcagcata tgctcgacag ttatgccgag
      601 caggcggata acatgccgtt attgcatcgg gcgctggtgc tggcgtgggc acaggagatg
      661 aatctgccgt gcaaaacgtt gttgaaaggg ttggatgaag ctatcgcccg gcgcggaact
      721 aaagatgaag atttctctga ggaagacatc cgcgatatca atgacagcct gatcctcgat
      781 acaccggaat ctccactggc agatgcggtg gcaaacgtct taaccatgac gttgctgaaa
      841 aaagcgcagt tgaagtccac ggtgatgcca caggttcagc aatatgcgtg ggataaagcg
      901 gcaaacagca atcagccgct ggcgcacacg gttgtgctgc tcaatagcgg tggtgacgct
      961 acccagacgg ccgctatttt aagtggtttg accgctgagc aatccactat tgagcgcgcg
     1021 ctggccatga actggctggc gaaatatatg gcgacaatgc ctccagttgt tttgcctgcg
     1081 cctgcgggcg catgggctaa acataagtta actggagggg gcgaagactg gcgttgggtt
     1141 ggtcagggtg tgccggacat tctctctttt ggtgacgaat tatcgccgca aaatgtgcag
     1201 gtccgctggc gtgaaccggc aaaaacggct caacaaagta acattccggt gaccgttgaa
     1261 cgccagttgt atcggcttat ccccggtgaa gaagagatga gctttactct gcagccggtg
     1321 accagcaatg agattgacag cgatgcgctg tatctcgatg aaattacgct taccagcgag
     1381 caggatgcag ttctgcgcta tggtcaggtg gaagtaccgc tcccgccggg agccgacgtt
     1441 gagcgcacaa catggggcat ttcagtcaat aaacccaacg ccgcgaaaca gcaggggcaa
     1501 ttgctggaaa aagcgcgtaa tgaaatgggc gaactggcct atatggtgcc ggtgaaagaa
     1561 ctgacgggaa cggtcacttt ccgccatttg ctgcgcttct cgcaaaaagg gcaattcgtt
     1621 ctgcctcctg ctcgttatgt gcgttcctat gcacctgcgc agcaaagtgt tgcggcaggg
     1681 agtgaatgga ccgggatgca ggtgaaataa gtgaactggc gcagaatcgt ctggctattg
     1741 gcgttggtta ctctgccaac gcttgcggag gagacgccgt tacaactggt gctgcgcggt
     1801 gcgcagcacg atcaactgta tcagctgtcg tcatcaggcg tgaccaaagt ctcagcatta
     1861 cctgactcgc tcaccacgcc gttagggagt ttgtggaaac tgtatgttta cgcctggctg
     1921 gaggataccc atcaaccgga gcagccctac cagtgtcgcg gaaactcccc ggaagaggtt
     1981 tattgttgtc aggctgggga gagcataacg cgcgataccg cgctggtacg ttcctgtggg
     2041 ctctattttg cgccgcagcg gttgcatatt ggcgcagatg tgtgggggca atactggcaa
     2101 cagcgtcagg cacctgcgtg gttagcgtct cttacaacgc tgaagccaga aacctcggta
     2161 acggttaaat cgctgctcga ttcattagcg acgttacctg cgcaaaacaa agctcaggaa
     2221 gtcttactgg atgtggtgct ggacgaggcg aaaatcggtg tcgcatccat gctcggtagt
     2281 cgggtacggg tgaaaacctg gagctggttt gccgatgata aacaggaaat ccgccagggt
     2341 ggttttgctg gctggttaac cgatggtaca ccgctgtggg ttactggcag tggcaccagc
     2401 aaaacggtac taacccgtta tgcgacggtg cttaatcggg ttttgccagt gccgacgcag
     2461 gttgccagtg ggcaatgtgt agaggtggag ctgtttgccc gctatccgct gaagaaaatc
     2521 acagcggaaa agagtacgac ggcagtaaaa cccggcgtac ttaatgggcg ctatcgtgtg
     2581 acgtttacta atggtaacca cattacgttt gtcagccacg gtgagacgac cttactgagc
     2641 gaaaagggca aactgaaatt gcagtcgcat ctcgatcgtg aagagtacgt tgccagagtt
     2701 ctggatcgcg aggcgaaaag tacgccacct gaggcagcga aagccatgac cgtggcgata
     2761 cgtacttttt tacagcaaaa cgccaatcgg gaaggtgatt gcctgaccat ccccgacagc
     2821 agcgccacgc agcgtgtttc tgcttcgcca gcgaccacgg gggcgcgaac gatgaccgct
     2881 tggacgcagg atctcatcta tgcaggcgat ccggttcatt atcatggcag tcgcgctacc
     2941 gaagggacac tttcctggcg gcaagccacg gcccaggcgg ggcaggggga gcgttacgac
     3001 cagatactcg cgtttgctta tcccgacaac agccttagcc gctggggagc tccacgctca
     3061 acctgccagt tattacccaa agcgaaagcc tggctggcga aaaaaatgcc gcagtggcgg
     3121 cgtatattac aaggcgagac ggggtacaac gaaccagacg tgtttgcggt ctgtcgttta
     3181 gtctcaggtt tcccctatac cgatcggcag cagaaacggc tgtttattcg taacttcttt
     3241 acgcttcagg atcggctgga tttaacccat gagtatctgc acctggcctt tgatggttat
     3301 cccaccgggc ttgatgagaa ctatatcgaa acgctgaccc gacaattatt aatggactaa
     3361 tgctatgcga aagatttttc ttccgttgtt actggtggca ctttcgcctg tcgctcacag
     3421 tgaaggtgtg caggaagtcg agattgacgc accgctctct ggctggcatc cggcagaggg
     3481 cgaagatgcc agtttttcac aatcgatcaa ttacccggcg tcgtccgtca acatggcaga
     3541 tgatcaaaat atctcagcgc agatccgcgg caaaataaag aattatgctg cggcgggtaa
     3601 agttcagcag ggccggctgg tggtcaacgg tgccagtatg ccgcagagaa ttgaatccga
     3661 tggttcattt gcacgccctt atattttcac tgaaggcagc aacagcgtgc aggtcatcag
     3721 cccggatggg caaagccggc aaaaaatgca gttttactca acgtcgggta taggaacgat
     3781 tcgtgcacgt ttacggctgg ttctctcgtg ggatacggac aataccgatc tcgatctcca
     3841 tgttgtcacg cccgatggcg aacacgcctg gtacggtaac accgtgctga aaaacagtgg
     3901 tgcactggat atggatgtca cgacggggta cggacccgag attttcgcca tgccagcacc
     3961 agttcacggg cgctatcagg tgtatatcaa ctactatggt gggcgcagcg aaacggaatt
     4021 gacgaccgcc caactgacgc tgatcaccga tgaaggatcg gtcaatgaga aacaggaaac
     4081 atttattgta ccgatgcgta atgctggcga gctgacgctg gtgaaaagtt ttgactggtg
     4141 attaatgggg ttaccttcgc cgtcgatgtt caagaaaacg cccgataact ttcgctatcg
     4201 ggtgttttta ttgattaatt caaccgttca atcaccatcg caattccctg gccgccacca
     4261 atgcacagtg ttgccagccc cagcgtttta tcgcgtgcct gcatggcatg taatagtgtg
     4321 accagaatac gagcaccact ggcaccgata ggatgcccga gcgcgatggc cccgccgttg
     4381 acattcactt tctcagaatc aaagcccagg gttttcccaa cggcgaggaa ctgtgcagca
     4441 aatgcttcat tagcttcgat gagatcaatg tctgccagtt gcagccccgc cagttgtaac
     4501 gctttttgcg tggcaggtac tggccccata cccatcaatg cgggaggcac gccaccgctg
     4561 gcataacttt taatgcgagc caggggggta aggcctgctg ccagcgccgc agattcttcc
     4621 ataatcacca gagcggcagc accgtcgtta ataccagacg cgttcccagc ggtgactgtt
     4681 cctgctttat cgaaggccgg gcgcaatgca cctaacgctt cagccgttga attcgctttc
     4741 gggaattcgt cttgactgaa gacgaaggtt ttcttccgag tgacaacatt taccgggacg
     4801 atttcggctg taaaagcacc ggactcaatt gcggctgccg ctttacgctg tgaatgtagc
     4861 gccagttcat cctgcatttc acgggtaatt ccgtactctt tagccacgtt ttcggcggta
     4921 atccccatat gataaccatg ggtggcgcac atcaggccat cgcgcaggat tacgtcataa
     4981 acctgtccgt ctccaagacg ataaccagag cgtgcttttg catcgagtaa gtagggcgct
     5041 aaactcatat tttccatacc ccccgccaca atgctcttcg cctgacctgc ctgaattgcc
     5101 tgggcggcaa gcgccacact tttaagaccc gaaccacaca ctttattgac cgtgaatccg
     5161 cacaccgttt ctgccagccc gctttttaac agtgcctgac gtgccggatt ttgccccaac
     5221 ccggcttgca acacgttacc cataatcact tcatcaatgt gtagtgaatc gatttttgca
     5281 cgttcaatgg cggctttaat tactgtcgcc cccaggtcga tggcgctggt ggaagcgagt
     5341 gaaccgttaa aactaccgat agcagtacgt accgcactga cgatgacaca atttttcatt
     5401 ttatattcct tctgtctgta gggttgccgt cagaacagcg ttaaaccaat gacgaaaatg
     5461 acgccggaga agagcagggc agtgatgcaa tagcccatga tgtcgcggac accgagtccg
     5521 gcgattgcca gtgctggcag cgcccagaat ggttgttcca tgttcatcca ttgctcgccg
     5581 taggcgatcg ccattaccga tttaccgaga tcagcgccca gcgcctgggc tgcgggtatc
     5641 acgaaaggtc cctgaataac ccagtgaccg ccgccagacg gaacggcgaa gttaatcagt
     5701 gcagaactaa aaaaggtcat taccgggaag gtgtctttgt tcgcaacatt gatgaagaat
     5761 tcggtaatga gtccacccag accagaatgc tccatcatca gttggatccc agcgtagaag
     5821 gggaattgca ccagaatacc ggcagtactg cgtgctgccg cgctgatagc acgcatataa
     5881 gccattggcg ttttatgtag caacagaccc gcaatcataa acatcaggtt gacggtattg
     5941 atggtgatgt tgaagccatg ttcgctgaag tacatcgcca ggtaagcgat accgagtgcg
     6001 ccgatgatca acgtcagaat gcggctttct tccagtcgct cggatggtgg ggcatctttc
     6061 ggtagctgct tttgaaaatc agcctcttcc atgagtagtt ccggatcgat actcaccacg
     6121 tcagacggtt ttggcatcat catgcgggtg ataaatggca tcaccacaat caacgtcaca
     6181 gtgatgaaaa tgttaaaacc actgaacaga gtatcgccca ccgggatcag cccggcgata
     6241 tgctcaaccg ggttgcccgg tgttgcagcc aacagaggca ttgatccaga gaagccgcca
     6301 ccccaggtga gaaaaccaat gtaggcgcag gcaatgagca acggataatc agaaccgggg
     6361 actcgccggg cgacttcacg ggcaaacatt gcgccgacaa ccaaaccaaa tccccagttg
     6421 atgacacaag cgactgaacc gaagaaagtg accagcatga cgccctgtac gggcgtcttt
     6481 gcggcggagg cggcagtacg cagcaaactt ttcaccggag cagagctggc aagggcatga
     6541 ccggtaacga tgataagcgc catctgcata ccaaacgcca gcaagttcca gaaaccgtca
     6601 ccccacattt tcaccatgct gatcggcgtt tgtggtgtta accaaagcgc gatcacgaat
     6661 gttagcaatg tcagcaacat ggcaaagatc agtggatcgg gaagccatcg gctgacaaaa
     6721 cgcgtcataa aacgcgatat gcgaccaatc ataaatcacc ccgttgcgta ttcagatcgg
     6781 cggcgacttc aaaccgagct tctgttttgg cacgcacggt ggctaaatca cacccgtcgg
     6841 caatttcggt gagccacatt ttgccgtcaa taaaacgaaa gacagccagt tcagtaacca
     6901 gcatatgcac cgcatgttgc gcagtgagtg gcatggtgca gctgcgcaaa atttttgctg
     6961 aaccatcttt ggcgcaatgt tccatggcga tgatcacttt gcgcgacccg gtcaccagat
     7021 ccatcgcgcc acccataccg ggcaccattt tcccaggcac tacccagttc gcgaggtttg
     7081 cttcttcgtc tacttgcaaa ccgccgagca cgcaggcatc aatatgaccg ccacggatta
     7141 gcgcaaatga catggcgcta tcaaacatgg ctgcaccggg taaaacaccg cacggttgcc
     7201 cgccagcgtt caccagatct ggatgcgctg tcgtgaccgg gcctaaaccg aggaagccgt
     7261 tttccgattg cagagtgata tgaataccct ccggtaaata attggcgacc attgtgggta
     7321 aaccgatccc taagttaacg atgtcaccat cacgaagctc ttgcgccaca cggcgcgcaa
     7381 tacgttgttt cgcatccatt atttgctctc ctgtgaaacg atgatgtggt cgataacggc
     7441 accaggggtg acaatatggt caggttgcag ctcgccggtt tcgaccagtt catctggctc
     7501 taccagcgtg atatcagccg caagggctat cagggggtta aagttgcggg cgctaagttg
     7561 ataggtcagg ttgccaagtg tgtcgcaacg atgagcgcga attagcgcca ggtcggcgcg
     7621 cagtgggcgt tcgagcagcc aggttttacc gtcgagtgtc agtgtctgtt tgccttcctc
     7681 tacgacggtg ccgacacccg ttggggtgag aaaaccacca agtccagctc caccacagcg
     7741 aatttgctcg attagcgtac cttgcggcac cagaacgacg tccatctcac cagatatcat
     7801 gcgccgacct gtttccgggt tggtgccgat atgtgaagca atcactttgc ggactcgacc
     7861 attgacgatg agcggaccga tgccggtatc aacaaacgcg gtatcattgg ctatcaatgt
     7921 caggtcgcga acaccagatt ccagtaatgc ttcaaccagg cgggatggag tgccaatccc
     7981 cataaatccg cccaccatga tggtcatgcc gtcacgaaag aagccggtgg cgtcttgtaa
     8041 tgtcatcaat tttgttttca ttttttatcc ctcttgcata caccgttgtg ggtacagtca
     8101 ggcaatagca aggggagtgc cagaatgaga ttattggatt tagttattta actaattgaa
     8161 tatgaaggga taaattggat ggggatttat aaggagaatg agcggctatg cagaaaattg
     8221 cgcactgtgc aaatttctgc atagcaagtt ttggtgttat acatccgccg gatcgatacc
     8281 gtattcctgg agtttataca tcaatgcacg gcgactgatg cccagcatta aagcagtgcg
     8341 ggttcggttt ccttcttgtt gttccagcac ttccataatg atgcgttttt cgacgcgttt
     8401 aatttcctct tttaaattac gctcaccgac agaggctgtt tttacctcgc cagcattaca
     8461 gactggctga cgaatctgtg gcggaagatc ctcagaaaaa atgatcgggc ctgaattcat
     8521 cacgacggcg cgttcaataa cgttagaaag ctctcgaata tttcccggcc atgaccaggc
     8581 ggtaagcagt gacattgcca tcggatcgat gtcgataata tcgcgctgat tctcactact
     8641 gaatttttgc aaaaagtgat tagctaacag ggaaatatct tcccggcgat cgcgcagagg
     8701 cggcagtatt aaatgaataa cgttaaggcg ataaaagaga tcttcacgga aggtgccttc
     8761 ttttaccatt gcctgcaagt cacggttggt ggcagcaatg atgcggatat caacttttat
     8821 ggtctgatgg ccgccaatcc gttcaaattc ccgttcctgt agaatgcgta gtaatttggc
     8881 ttgtagtacc agcggcattt cgccaatttc gtcgaggagc agagtacctt cgttggctcg
     8941 ttcaaataat ccctgacgca aggtttgtgc accagtaaat gcaccttttt catgaccaaa
     9001 cagttcactt tcgagcaacg attccggcag cgccgcgcag ttgactttaa tgaacggccc
     9061 ctttgcccgc cgcgaattgt agtgaatcgc tctggcaatc aactctttcc cggtgccgct
     9121 ttcaccgcta atcaagacgc tggcctgaga aagggcaatt ttggcggtgt ctttgcagat
     9181 gtccatcatc gccgggctgt tggtgagaat gtgcccccat tgccagctgg cgctcagtgc
     9241 ctggtgcaga tgacggatct cttttttcat tgactggagt tgtaaagcgc gctgaacgat
     9301 taaattcaac tcatcgagat caaacggttt aataacatag tcgaaggctc cgcagcgtag
     9361 cgcttcgacg gcggtttcca cttccgcata ggccgtcatc agaataacgg gtgtccgggt
     9421 ctcatggctg cgcatctcct ttagtgcctt gatgccgtcc atctctggca tgcggatatc
     9481 catcaacacc acatcagggt gaatatcggc aaacaggtgt aatgctgtgc gtccgttgtt
     9541 cgcacaatgt gtttcgaatc cttgtagtgc aaaagcggtg ctcagcatac ggcgaacatt
     9601 atcttcatca tccacaataa ggatgcgatt aatagcagtc atacagtctg atttccctgc
     9661 gggttgatcg gtaaaataag cgtgaaggtt gcgccgtagc ccggcaaact ggcgacgcga
     9721 atatcaccct gatgggcatt aatgatgcgt tgacttaacg ccagaccaag cccggttcct
     9781 gaggctttgg tggtgaaaaa gggatcgaag atcttttttt gcagcgagag atcaatgcca
     9841 cagccgttgt cctctatcga aatggcctgt tgtgagtcgc tgtattgcca ggtttgaatg
     9901 cgaattttcc ctcgtgcgct gatagcctgg acggcattga tcaggatatt cagtagtacc
     9961 tgtttgagca gttcacgatc ggcgttaatc gggcttaatt cattatccag ttcgcttatg
    10021 aagtcgaccc gcgcttgtac gccggcggtt tgtaccagta ccagagtttc ttcaaccaat
    10081 gcattgaggc tgacttgttg ccattgactg tggcgtggac gtgaaaattc gagcaattgc
    10141 tgaataactt tgttaattga atcgatttct ttgagtacta cggacagata ttcctgatgt
    10201 attgggtcac tggtttgttg gcgcaagatc tgtacataac cacgaatagc cgttaacgga
    10261 ttacgtactt catgcgcgac gccagccatc agctcaccca gtgtggcgag gcgttctgct
    10321 tgcgccatgc ggcgctgggt ttctttgcgg gcagttaaat cagagaaaat caccaaagca
    10381 cctatcattt caccgtgcgt gttatgaata cgactggtag tgacactgag ttcaatggtg
    10441 cggtcacgac ctggaaaact gatctccagc gccacatgtt cggtgccatg ttccagcgta
    10501 tccagtactg gactgtagaa ctgagtattg tcgaacaaca tggagtaagg ctgccctacc
    10561 agttcatggc gttgatagcc agtgataact tctgctgctg ggttcatggt ggttacatca
    10621 ccctggcggt caatggcaat gacgccatcg gcagcgtttt caataatcag atcgttaagt
    10681 gtccgcgttt cacgcagtgc ctgggcgagg ttattaacac tctgactgat ttgccccatt
    10741 tcaccgggca attgtggtaa tcgagtggga atattttgtg ccagagtcga gaggccatcg
    10801 gtgatgatat caatattggc gctcaggcga cgggagaaaa ggacaatcaa tagcaggctt
    10861 atcagcaaat cggcggtgag cacaatgata atcctcacat ccattttcca ggcctggcgg
    10921 cgaatatctt cggttaattc attggcccag atatagccga ggatttcacc attacgctca
    10981 atggggagca ttgaattcaa aatatcgccg cgcacctgcc tgcctgaata aaccaaaggg
    11041 gtatttgtac gcatgacttc gcgaccaggg tgatctgcgg caatggtaac gccgacatta
    11101 ttctgatata gcgctgaagg cgcgtaggtg attatcgcat ccagcatttt gttgtaataa
    11161 ccagcaccta tgccagggaa ggcgtgagtg atattttcgg taatgggggc aagttctgca
    11221 tttaatgcgc ggatacgctc ctcacgtggt aagtcgatgt agagatcata gcgatcgcct
    11281 agtgcctgat taagcaggtt gaccacggca gaaagttttt tctctttttc agataagact
    11341 gctgaacgtc cttccgtttc tacgatataa ccaatagtaa gcgttgggac aatgaccatc
    11401 aggattgcca tcaggatcat ttgattgcgt aagcggcgtg gataaatcca cttcatataa
    11461 tgcatgctga aatccactag tcttgtccgg tatatgacga ttatcagagg ttaaggtgat
    11521 gatttctcgg cggtgtatca tattccagag aagagagaac attgcggtaa cacgctttta
    11581 ccgctacctt aaccacactc catcggtcac ctgaggcgga gcttcgcccc tttgaaatac
    11641 cttgcttctt ttcgtacaac cctgaaagcc tcgcgctaca tgttcagagc attggcgtta
    11701 gtgctctggc tgttgattgc tttttcatcc gttttttaca tcgttaatgc gttacatcag
    11761 cgagaatcgg aaattcgtca ggaatttaat ctgagttccg atcaggctca gcgctttatt
    11821 caacgcacct ctgatgtgat gaaagagctg aagtacatcg ccgaaaatcg cttatcggca
    11881 gaaaacggtg tgctttcccc gcgtggacga gaaacgcagg cggatgtgcc tgcgtttgaa
    11941 ccgctgtttg ccgactccga ttgttccgca atgagtaaca cctggcgagg ttctctggag
    12001 tcattggcgt ggtttatgcg ctactggcgc gataattttt ctgcggctta cgatctaaac
    12061 cgggtatttt taatcggcag cgataacctc tgcatggcca atttcggtct gcgtgatatg
    12121 ccagtggaac gcgataccgc gttgaaagct ttgcatgaac gcatcaataa atatcgaaat
    12181 gcaccacaag atgatagcgg cagtaacctc tactggatca gcgaaggtcc gcgccctggc
    12241 gtcgggtatt tttacgcgtt gacgccagtt tatctggcga accggttgca ggcgcttttg
    12301 ggtgtcgagc agaccatccg gatggagaac tttttcttac cgggtacgtt gccgatgggg
    12361 gttaccattc ttgatgaaaa tggtcatacc ctgatttcgc ttaccggacc agaaagtaaa
    12421 attaagggcg atcctcgctg gatgcaggaa cgctcctggt ttggctatac ggaagggttc
    12481 cgggagctgg tgctgaagaa aaatctgcca ccctcatcgc taagcatcgt gtattcggtg
    12541 ccggttgata aggtgctgga acgcattcgc atgttgatcc ttaacgcaat tttgctgaat
    12601 gtgcttgccg gagctgcatt gtttactctc gcacggatgt acgagcgacg tattttcatt
    12661 ccggcggaaa gcgacgccct gcgactggaa gaacatgagc agttcaatcg caagattgtc
    12721 gcctccgcgc cagtgggtat ctgcattttg cgtaccgctg atggcgtcaa tattttaagt
    12781 aacgaactgg cgcataccta tctcaatatg cttacgcatg aggaccgcca acgactgacg
    12841 caaattatct gtgggcagca ggtcaatttt gttgatgtcc tgaccagcaa caataccaat
    12901 ctgcaaatca gcttcgtcca ttcgcgctat cgtaatgaaa acgtggccat ttgtgtgctg
    12961 gtggatgttt cttcgcgcgt gaagatggaa gagtcgttgc aggagatggc acaagcagcg
    13021 gaacaggcga gccagtcaaa atcgatgttc cttgccaccg tcagtcatga gctgcgaacg
    13081 ccgctgtatg gcattatcgg taacctggat ctgttgcaaa ccaaagagtt accgaaaggc
    13141 gtcgatcggc tggtgacggc aatgaacaac tcttccagcc tgttgttgaa aattatcagc
    13201 gatattctcg atttctcgaa gattgaatcg gaacagttga agatcgaacc gcgtgagttt
    13261 tcaccgcgtg aagtgatgaa ccacatcacc gccaactatt taccgctggt ggtacgcaag
    13321 cagttaggct tgtactgctt tattgaaccg gatgtgccag tggccttaaa tggcgacccg
    13381 atgcgtttac agcaggtcat ctccaacctg ttgagtaacg ccataaaatt caccgatacc
    13441 ggctgtatag ttttgcatgt ccgcgctgat ggcgattatc tctctatccg tgttcgcgat
    13501 accggcgtgg ggattccggc gaaagaagtg gtgcgcttgt ttgatccctt cttccaggtc
    13561 ggaacgggcg tacagcgaaa cttccagggg accggtctgg gtctggcgat ttgtgaaaaa
    13621 ctgatcagca tgatggacgg cgatatctcg gtagattcag aaccgggaat gggcagccag
    13681 tttaccgtgc gtattccgtt gtacggcgct cagtacccgc agaaaaaagg cgtggaaggg
    13741 ttgagtggta aacgctgctg gctggcggtc cgcaatgcgt cgctctgtca gttcctggaa
    13801 accagtttgc agcgcagcgg catcgtcgtt acaacatacg aagggcagga accgactccc
    13861 gaagatgtgt taattactga cgaggtagtg agtaaaaaat ggcagggtag agcggtagtg
    13921 accttctgtc gtcgccatat tggtattccg ctggagaaag cgccagggga gtgggtacac
    13981 agtgtggctg ccccgcatga gctaccggca ttgttggcgc gtatttattt gatcgagatg
    14041 gagagcgacg atcctgctaa cgctctgccg tcgacggaca aagcggtcag cgataatgac
    14101 gatatgatga ttctggtcgt ggatgatcat ccgattaacc gacgtctgct ggcagatcag
    14161 ttgggatcgt tgggctatca atgtaaaacc gcgaatgatg gtgtcgatgc gcttaatgta
    14221 cttagcaaga atcatattga tatcgtgctt agcgacgtca acatgccaaa tatggatggt
    14281 taccgcttga cgcaacgcat tcgtcagttg ggactgacgt tgccggtaat cggagtaact
    14341 gctaatgcgt tggctgaaga gaagcagcgg tgtctggagt ccggtatgga cagctgcctg
    14401 tcgaagccgg taacgctgga tgtgataaaa cagacgctga cggtatatgc cgagagggtc
    14461 aggaaatcgc gggaatcgta ggacggataa ggtgtttatg ccgcatctgg cattcagtgc
    14521 aaatgccaga tgcgacgctg acgcgtctta tctggcctac tttgatgtaa tctgttgaaa
    14581 taatgggaat cgtaggacgc ataaggcgtt tacgccgcat tcggcaatcg gtgcaaatgc
    14641 cagataagac actaacgcgt cttatctggc ctacaggtga ttagtcttta tctgccggac
    14701 ttaacgtcac tgaagagaga taattcagca gggcgatatc gttctcgaca cccagcttca
    14761 tcatcgcaga tttcttctgg ctactgatgg ttttaatact gcggttcagc tttttagcga
    14821 tctcggtcac caggaagcct tccgcaaaca ggcgcagaac ttcactctct tttggcgaga
    14881 gacgcttgtc accgtaacca ccagcactga ttttttccaa caggcgagaa acgctttccg
    14941 gggtaaattt cttccctttc tgcagcgcgg cgagagcttt cggcagatcg gtcggtgcac
    15001 cttgtttcag cacgatccct tcgatatcca gatccaatac cgcactaaga atcgccgggt
    15061 tgttgttcat agtcagaaca atgatcgaca ggcttgggaa atggcgcttg atgtacttga
    15121 ttaaggtaat gccatcgccg tacttatcgc caggcatgga gagatcggta atcaacacat
    15181 gcgcatccag tttcggcagg ttgttgatca gtgctgtaga gtcttcaaat tcgccgacaa
    15241 cattcaccca ctcaatttgc tcaagtgatt tgcgaatacc gaacaagact atcggatggt
    15301 catcggcaat aattacgttc atattgttca tgtaataggc taccttgcta cagcaagctc
    15361 ttgacataac tgtcaatgtc gctgatgtat ttttctattc ctggaacatc cttctcacga
    15421 atcagatgtt ccagcgtttc acataactgc ttgccgggta ccagatttag catggcaaat
    15481 acgcctttaa gacgatgagc cgtttgggct aacgcagcaa agtcactggt tgctgcttca
    15541 gtatacagcc tcttaacatc atccggtact gtgtctacaa agagcgcata atagccgctg
    15601 gcatggagtt gcgcattttc atctccgccc agaggcgatt ctgtcacctc ttcctgcgcc
    15661 agttgcactt caattaattg taagaccgct tcctgcatag cgttgctcat attgaagttg
    15721 acgcacaatt gaccaggccc aatttcccgt acgccagact catcatcgct taaaagcaag
    15781 ccagaggcag taagattaga cggattatcc gttaaaaaga tatcataatc ttgactaatt
    15841 aatctttcat cgggtgtgat acaggttgca ccccaatttt ctaactggcg agtgacaata
    15901 ttccgaattt ctgccgaagt aacatccacc attacgcaga catcatccag taaacgctct
    15961 tcttcctctt caacttccgg gccagcggcg agcattttga tatgcacaga gtagcgtgta
    16021 ccaagcccat cccgcgtttt gatgtttaaa tgaccgccca gtttacgtgc cagttgatcg
    16081 ctcagccaga atgccagcgg gtccgccttt ccataacgat cgttttgggt ctggttgata
    16141 aacgggaagt gcaaattatc catttcatga atacttacgc cttctccggt gtccagaatg
    16201 cggaatgtca ggcggtcttc agaggactca tcctgatcaa cctcaagggt gatttttccc
    16261 aattgcgttg aggtcacggc atattgcatc agtagcagca aaatacgtcg taaggcatcg
    16321 cgatcgccgc ggcgcatatc gtgtgctttc agatgattgt taatcagcag ttgcagacct
    16381 ttacgcttga tggcaggcaa cactgaaggc acaacttcat caattaaatc ctgcacggag
    16441 aacagcaccg tctcactttt ccagctatcg tccgcaagca tgttcgctaa ctgtatttca
    16501 tcgaccagcc gcaccagcac atctgcctga ttcgccagtt gtttgctttc cggggcgttg
    16561 agtttagccg cgctctccgc cagggactgt gcaggttctt tcagcgcatc gccaatgttt
    16621 ttcataaagg tcatccggcc ctgctggttt ttctcataca gacgctgcgc ctgcttgagt
    16681 ttcttgttta ccagcacttc gcgatcctga tcgcgaataa tgaaaatttg tgtgcgcggc
    16741 gcgacctggc tgcggaacat gcggatctca tacagctcgt tattgatcgt cgcctgaata
    16801 atcccctgat gctgttccgc catggtggtg atgttttgca gattcaaatg cggcagcaaa
    16861 tgatcggcaa ttttgttact tatgacagtg cggttcgatt cctgatcgtg aaccagcagg
    16921 ccgagcggca gcagtgagac tatcttttca ttgattgccc gtaaaatgcg caattcgtta
    16981 ttgaccgccg tgttgggtag actttctgta ctgcgactgg agaaatggcg gaatgtggta
    17041 tagccaaata acgccagcgc cagcaaaccg atgttcagca gcagtggcag cagaatgttt
    17101 tgcaacgtat ccagcaataa ggtgccataa ggaacctgcc agaccaggcg catatcggta
    17161 gagttgagtg ccgaggagat ttcaatcttc gtactgttaa agtggatact gacgctatcc
    17221 gttccttctt tctcattatc attgtctccc gttgccgtcg tgtctggctc aaggcggaaa
    17281 ctgtccagcg gcatacccgg tgggatcaaa tcattaatcg gcagatcaaa agccacgacc
    17341 gttgccagat gtcccggctg gttgaacgtg gtacgcaagg taaagtaatg accgttctgc
    17401 caggccaggc ggcgcatgtt agaaaaactt tcgcgttcat cgagggcgtt ggcctgttgc
    17461 aacatttctg cacgacgtga atcaacaatg tcactgacgg tcgattcttt aaatccggag
    17521 gtgagatctt tgaggggcag ggttgagatc agtaccagac tgttatcctg accattcagg
    17581 taatacatcg accacggtac attttctgca ccccacaatg tatccagata agtggacatc
    17641 cgctgagtca tctcaagcgt tgagttgtcg tgagagccaa agattaacgc ttccgttttg
    17701 cggcgtggtt tctccagata gtagacatcc tgtttcaggc gcgtctcttg taaaccttcg
    17761 ccggaggagg gggaggtcgt cgcggcaatg ttgtcgtaga tctgccaggt cacgtaacgc
    17821 caggtatcga cgcgcttttg gattgcgtgg gtaatgtcga caatctggta acttttatct
    17881 ttcagccagg cgttaacggc gctttgtacc attaccccca tcgtcaccag taacacaatg
    17941 atcaacagta aaaagaagcg ggtaatgctc cccggtagga gtgaaaagcg ggtcgtggcc
    18001 gttgtctctt tctgacgcat tcgtgtttat gacctgttaa aacttcgcga agtgataggg
    18061 cagtataaag ggtacagtgt gaaattccag actcttacct gattgatgat tctctattct
    18121 cccgtgatat ttatcatcct gcattggggg aaatagatgt acaagcgcca ttttgctgta
    18181 caaataacct acaaaaagcc caacgaaatt aattgcaaat aaacgaagat tgctggaaat
    18241 tatgctgatg ttaattattt gtgaaatagt tgacaagagt tatagttttt ctgtggtagc
    18301 acagaataat gaaagtgtgt aaagaagggt aaaaaaaacc ggatgcgagg catccggttg
    18361 aaataggggt aaacagacat tcagaaatga atgacggtaa taaataaagt taatgatgat
    18421 agcgggagat attctagttg cgagtgaagg ttttgttttg acattcagtg ctgtcaaata
    18481 cttaagaata agttattgat tttaaccttg aattattatt gct
//
LOCUS       DADMKF010000073        18518 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.074, whole genome shotgun sequence.
ACCESSION   DADMKF010000073 DADMKF010000000
VERSION     DADMKF010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18518
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.074"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            78..290
                     /gene="cspH"
                     /locus_tag="JNC24_002343"
     CDS             78..290
                     /gene="cspH"
                     /locus_tag="JNC24_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HAY9159074.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
     gene            737..1042
                     /locus_tag="JNC24_002344"
     CDS             737..1042
                     /locus_tag="JNC24_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9159075.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAASEATNGTEATTGTTGTTTTTTG
                     ATTTATTTGGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
     gene            1149..1793
                     /gene="gfcB"
                     /locus_tag="JNC24_002345"
     CDS             1149..1793
                     /gene="gfcB"
                     /locus_tag="JNC24_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein GfcB"
                     /protein_id="HAY9159076.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVADQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNSGQDPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLNIAGRNV
                     LCNVWQEEVTSTRPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            1790..2536
                     /locus_tag="JNC24_002346"
     CDS             1790..2536
                     /locus_tag="JNC24_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HAY9159077.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPGEQQTLSVGPMEN
                     VVQLVTQPQLRDRLWWPGALLTDSAAKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGAG
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVMVITPEGETVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            2536..4632
                     /locus_tag="JNC24_002347"
     CDS             2536..4632
                     /locus_tag="JNC24_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HAY9159078.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAYQPAPESEGLQYTTVANQLTALKYNAGFEAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGQSEQLQQQRVEAEDLSAFGRGYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDGGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKRFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLYPMTAEREVPVGQ"
     gene            4678..5817
                     /locus_tag="JNC24_002348"
     CDS             4678..5817
                     /locus_tag="JNC24_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HAY9159079.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            5805..6251
                     /gene="etp"
                     /locus_tag="JNC24_002349"
                     /gene_synonym="yccY"
     CDS             5805..6251
                     /gene="etp"
                     /locus_tag="JNC24_002349"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HAY9159080.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     IKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYGMLERASQEWAKRLSR"
     gene            6271..8451
                     /gene="etk"
                     /locus_tag="JNC24_002350"
     CDS             6271..8451
                     /gene="etk"
                     /locus_tag="JNC24_002350"
                     /EC_number="2.7.10.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HAY9159081.1"
                     /translation="MTTKNMNTPPGSTQENEIDLLRLVGELWDHRKFIISVTALFTLI
                     AVAYSLLSTPIYQADTLVQVEQKQGNAILSGLSDMIPNSSPESAPEIQLLQSRMILGK
                     TIAELNLRDIVEQKYFPIVGRGWARLTKEKPGELAISWMHIPQLNGQDQQLTLTVGEN
                     GHYTLEGEEFTVNGMVGQRLEKDGVALTIADIKAKPGTQFVLSQRTELEAINALQETF
                     TVSERSKESGMLELTMTGDDPQLITRILNSIANNYLQQNIARQAAQDSQSLEFLQRQL
                     PEVRSELDQAEEKLNVYRQQRDSVDLNLEAKAVLEQIVNVDNQLNELTFREAEISQLY
                     KKDHPTYRALLEKRQTLEQERKRLNKRVSAMPSTQQEVLRLSRDVEAGRAVYLQLLNR
                     QQELSISKSSAIGNVRIIDPAVTQPQPVKPKKALNVVLGFILGLFISVGAVLARAMLR
                     RGVEAPEQLEEHGISVYATIPMSEWLDKRTRLRKKNLFSNQQRHRTKNIPFLAVDNPA
                     DSAVEAVRALRTSLHFAMMETENNILMITGATPDSGKTFVSSTLAAVIAQSDQKVLFI
                     DADLRRGYSHNLFTVSNEHGLSEYLAGKDELNKVIQHFGKGGFDVITRGQVPPNPSEL
                     LMRDRMRQLLEWANDHYDLVIVDTPPMLAVSDAAVVGRSVGTSLLVARFGLNTAKEVS
                     LSMQRLEQAGVNIKGAILNGVIKRASTAYSYGYNYYGYSYSEKE"
     gene            complement(8566..9864)
                     /locus_tag="JNC24_002351"
     CDS             complement(8566..9864)
                     /locus_tag="JNC24_002351"
                     /EC_number="3.1.3.2"
                     /EC_number="3.1.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AppA family phytase/histidine-type acid
                     phosphatase"
                     /protein_id="HAY9159082.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESVVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQS
                     GQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILSRAGGSIADFTGHRQTAFRELERVLNFPQSNLCLKREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIKTALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(9944..10036)
                     /gene="cbdX"
                     /locus_tag="JNC24_002352"
     CDS             complement(9944..10036)
                     /gene="cbdX"
                     /locus_tag="JNC24_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HAY9159083.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            complement(10049..11185)
                     /gene="appB"
                     /locus_tag="JNC24_002353"
     CDS             complement(10049..11185)
                     /gene="appB"
                     /locus_tag="JNC24_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HAY9159084.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            complement(11197..12741)
                     /gene="appC"
                     /locus_tag="JNC24_002354"
     CDS             complement(11197..12741)
                     /gene="appC"
                     /locus_tag="JNC24_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HAY9159085.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKTVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLPLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            complement(12875..13732)
                     /locus_tag="JNC24_002355"
     CDS             complement(12875..13732)
                     /locus_tag="JNC24_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HAY9159086.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPISCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSQRQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSESEIQEAIFCGLWRVRKRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            complement(13789..14127)
                     /gene="hyaE"
                     /locus_tag="JNC24_002356"
     CDS             complement(13789..14127)
                     /gene="hyaE"
                     /locus_tag="JNC24_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HAY9159087.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSEFRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLREFPGYTWQVAIADLEQSEAIGDRFGVFRFPATLVFTGGN
                     YRGVLNGIHP"
     gene            complement(14124..14711)
                     /locus_tag="JNC24_002357"
     CDS             complement(14124..14711)
                     /locus_tag="JNC24_002357"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HAY9159088.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQAALAQLAAWGIVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTLEEQG"
     gene            complement(14708..15414)
                     /gene="hyaC"
                     /locus_tag="JNC24_002358"
                     /pseudo
     CDS             complement(14708..15414)
                     /gene="hyaC"
                     /locus_tag="JNC24_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309157.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
     gene            complement(15433..17226)
                     /gene="hyaB"
                     /locus_tag="JNC24_002359"
     CDS             complement(15433..17226)
                     /gene="hyaB"
                     /locus_tag="JNC24_002359"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HAY9159089.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFNKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNTSPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            complement(17223..18341)
                     /gene="hyaA"
                     /locus_tag="JNC24_002360"
     CDS             complement(17223..18341)
                     /gene="hyaA"
                     /locus_tag="JNC24_002360"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HAY9159090.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAAWPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDCGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
BASE COUNT         4544 a         4914 c         4783 g         4277 t
ORIGIN      
        1 cggcggtctt aaatatcgct ggctttattt tattaacccc gtatagtgca gcgcagtatt
       61 ttcagtaagg aatttgtttg tcccgtaaaa tgacaggaat tgtcaaaacc tttgatcgca
      121 aaagcggcaa aggattcatt atcccctccg acggtcgtaa agaagttcag gtccatattt
      181 ccgcattcac tccccgcgac gcagaagtgc ttataccagg attacgtgtg gaattttgcc
      241 gcgtaaatgg cctgcgagga ccaacagcgg caaatgttta tctctcttaa tatgacgctg
      301 cttgtttaaa gctgactggt tttattggaa tagtgcaata gtcaacatca tacattataa
      361 tatcagttac ttttccacaa agaaactaat ccatttaagc gaatatctct gcacctaata
      421 gttataaatg ccatttaaca gccagttata gtaccacttg aaactatatt accaacaata
      481 attaatagct ttcgtaacaa tttccttatc tttaatttgt attttgctaa gatttatctt
      541 atgctcacaa gtcgtatttc cagaggggat cgttaataat cttatatttc cctctgcgca
      601 cctttcctac ccgcagtatc agtatatagt gcaagcggta tgaggaaagg gagatttcac
      661 aaggatgtta tactcaaagt agacaatcct gatctcaagc gtacgtattg tccatgcgat
      721 gaataaagga aaagttatga aacacaagct ttctgcaatc cttatggcct tcatgttgac
      781 gacgccagct gcgtttgctg cctctgaagc aacaaacggg accgaggcaa cgacgggtac
      841 tactggcacc acaacgacca ctaccggcgc aaccacgact gctactacca ctggtggcgt
      901 ggctgctggc gctgtcggca cggcgacagt tgtcggtgtg gcaacggctg ttggtgtggc
      961 gacgttggca gttgttgcag caaatgacag tggcgatggc ggttctcata acacctcaac
     1021 caccacgtcc actacacgct aatactgctt taacgcgtcg aaattaaagt taaatgatac
     1081 ccggttgcga tattcgtacc gggctttaga gatttgcatt tttttaatta acctttcaag
     1141 gattaaaggt gcgccctctt attttatcga ttttcgcact atttcttgcg ggatgtacgc
     1201 acagccagca aagtatggtc gatacatttc gcgccagcct tttcgataat caggatatca
     1261 ccgtagcgga tcagcagatc caggcgttgc cttattccac gatgtattta cgccttaatg
     1321 aagggcaacg aatctttgtg gtactgggat atatagaaca agaacaaagc aaatggttat
     1381 cccaggataa cgccatgctg gttacccaca atggacgtct tttaaaaacc gtcaaactta
     1441 ataataatct gctggaagtg acaaattccg ggcaggaccc tctgcgtaac gcgctggcaa
     1501 taaaagatgg cagccgctgg acgcgcgata ttctctggag tgaagacaac cattttcgct
     1561 ctgcgaccct gagttctact ttttcctttg ctggattaga gacgctgaat attgcgggtc
     1621 gcaatgtgct gtgtaatgtc tggcaggaag aggtgacttc cacgcggcca gaaaaacagt
     1681 ggcaaaacac attctgggtc gattcggcta ctggccaggt tcgtcaaagt cggcaaatgt
     1741 taggcgcagg ggttattccc gtagaaatga cgtttcttaa acccgcacca tgaataaatt
     1801 acagtcgtat ttcattgcca gcgtacttta cgtaatgaca ccccatgcct ttgcgcaagg
     1861 aacggtgact atttatctgc ctggcgaaca acagacactt tccgttggcc ccatggaaaa
     1921 tgttgtccag ttggtgacac aaccgcaact gcgcgatcgc ctctggtggc ctggtgcctt
     1981 gctgaccgac tctgcggcga aagctaaagc gcttaaagac taccaacatg tcatggcaca
     2041 gcttgcttcc tgggaagcag aagctgacga cgatgtggcg gcaacgatta aatccgtgcg
     2101 ccagcagtta ctcaatctca atatcactgg tcgcttaccg gtcaaactgg accctgattt
     2161 tgtacgagtg gatgagaaca gcaatccccc gttggtcggg gactatacgc tctataccgt
     2221 acagcgtccg gtgactatta ctctgctggg tgcggtttct ggggccgggc aattgccctg
     2281 gcaggcaggc cgtagcgtta ccgattatct gcaagatcac ccccgccttg caggcgcgga
     2341 caagaataac gttatggtga ttacgcctga gggtgagact gtcgtcgccc ctgttgccct
     2401 gtggaataaa agacatgtgg aaccaccgcc tggcagccag ctttggttgg gcttttctgc
     2461 gcatgtactg cccgaaaaat atgctgattt aaatgaccag attgtgtctg ttttaacgca
     2521 acgtgttcct gactgatgaa gaagaattct tatcttttaa gctgcctggc cattgccgtc
     2581 tccagtgcct gtcatgctga agtattaacc tacccggatc cgctgggttc gtcgcaatca
     2641 gactttggcg gcacaggatt gttgcagatg ccaaatgcgc gcatcgcacc ggaaggtgaa
     2701 ttcagcgtca actaccggga taacgatcaa taccggttct actccacctc cgtggcgctg
     2761 ttcccatggc tggaaggcac cattcgttat acggatgtgc gcacacgcaa atatagccag
     2821 tgggaagatt tcagcggcga tcagtcatac aaagacaaat cattcgattt taaacttcgc
     2881 ctgtgggaag aaggttactg gctaccgcaa gtggcgtttg gtaaacgtga tattgctggt
     2941 acgggtctgt ttgacggtga gtatctggtg gccagcaagc aagcggggcc atttgatttc
     3001 accctcggga tggcatgggg ctacgccggt aatgcgggca atattaccaa cccgttttgc
     3061 cgggtgagcg ataaatattg tcatcgcgca gagtctcacg atgcgggcga tatcagcttt
     3121 agcgatatct ttcgtggccc ggcttccatc tttggcggca ttgagtatca aacgccgtgg
     3181 aatcccctgc gtctgaaact cgaatacgat ggcaacaatt accagaatga tttcgctggc
     3241 aaactgcctc aggcaagcca tttcaacgtc ggcgcagttt atcgcgctgc cagctgggca
     3301 gatctcaacc tgagttatga acgcggtaac acgttgatgt ttggcttcac gttacggacc
     3361 aatttcaacg atctgcgccc tgccctgcgc gatacgccaa aaccggcata tcaacctgcg
     3421 cctgaatctg aaggattgca gtacaccaca gtagcgaacc aacttaccgc gctgaagtac
     3481 aacgcaggtt ttgaagcacc ggaaattcag ctgcgcgata agacgctgta tatgtctggt
     3541 caacaataca aataccgtga ttctcgcgaa gcggtcgatc gtgccaaccg gattctggtg
     3601 aataacctgc cgcaaggcgt tgagaagatt agcgtgacgc aaaagcgcga gcatatggcg
     3661 atggtgacta ccgaaaccga cgtagccagc ctgcgcaaac agctggctgg tacagcgcct
     3721 ggtcaatcag agcaacttca acaacaacgt gttgaagcag aagatctttc tgcctttggt
     3781 cggggctacc gtattcgtga agatcgcttt agctactctt tcaacccaac actttcacag
     3841 tcgctgggcg gcccggaaga tttctatatg ttccagctgg ggctgatgtc cagtgcccgc
     3901 tactggttta ccgaccacct gctgcttgat ggcggtattt tcaccaatat ttacaacaac
     3961 tacgacaagt ttaagtcttc gctgttgccc gcggactcta ccctgccccg cgtgcgcacg
     4021 catatccgtg attacgttcg caatgacgtt tatctcaaca acttgcaggc gaactacttt
     4081 gccgacttag gcaatggttt ctatggccag gtgtatggcg gttatctgga aacgatgtac
     4141 gccggtgtcg gttccgagct gctttatcgc ccgctagatg ccagctgggc gctgggtgtg
     4201 gacgttaact acgtgaagca acgtgactgg gacaacatga tgcgcttcac cgattattcc
     4261 acgccaactg gtttcgtgac ggcttactgg aacccgccga cgctcaatgg cgtactgatg
     4321 aaacttagcg ttgggcaata tctggcaaaa gataaagggg caacgatcga cgtcgccaaa
     4381 cgctttgaca gcggcgtggc ggtaggggta tgggcggcaa tcagtaacgt atctaaagat
     4441 gactacggcg aaggcggctt tagtaaaggt ttttatatct cgattccgtt cgacttgatg
     4501 accattggac ctaaccgcaa ccgcgcggtg gtttcgtgga caccattgac gcgtgatggt
     4561 ggacaaatgc tgtcacgcaa ataccagctc tatccaatga cggcagagcg agaagtaccg
     4621 gttggacaat aacctgacgt aattcataac attacattga ctgataaata taaaccaatg
     4681 aaaaagaaca tttttaagtt ttcggtactg acattggctg ttctgtcttt gacagcatgt
     4741 acccttgttc ctggtcagaa tttatcgacc agtaacaagg atgtcattga actacctgac
     4801 aatcagtatg acctggacaa aatggtcaat atctatcccg tcacgccagg attgattgat
     4861 cagttgcgcg ccaaaccgat tatgtcgcag gctaacccgg agctggagca gcaaatcgca
     4921 aactatgaat atcgtatcgg gattggtgat gtgttgatgg ttacggtctg ggatcacccg
     4981 gaactaacca cccccgcagg gcaataccgt agcgccagtg ataccggcaa ctgggttaac
     5041 gccgatggtg cgattttcta cccctatatt ggtcgcctga aagtggccgg gaaaacactt
     5101 acccaggtac gcaacgagat tacagcgcgt ctcgattcag tcattgaaag cccgcaagtc
     5161 gatgtcagcg tcgcggcgtt ccgatcgcaa aaagcgtacg tcaccggcga agtcagcaaa
     5221 tcgggtcagc agccgattac caatattcct ctgaccatca tggatgcgat taacgccgcg
     5281 ggcggtctga ccgcagatgc agactggcgc aacgtggtgc tcacccagaa tggcgtaaag
     5341 accaaagtga acctctatgc gctgatgcag cgtggcgact tgcgccagaa caaactgctg
     5401 cacccgggcg atattttatt tattccgcgc aacgatgatc tgaaagtctt cgtgatgggt
     5461 gaagtgggta aacagagcac gctgaaaatg gaccgcagcg gtatgaccct ggcggaagcg
     5521 ctgggtaacg ctgagggtat gaatcaggat gtcgctgatg cgacggggat ttttgttatt
     5581 cgtgcaacgc aaaacaagca gaatggcaaa atcgccaata tttatcagct gaatgcgaag
     5641 gacgcatcgg caatgatact cggaacggaa ttccagcttg aaccttacga cattgtttat
     5701 gtcactaccg cgccgctggc tcgttggaac cgtgtgattt ctctgctggt accgacgatt
     5761 tccggggtgc atgacctgac agagacttca cggtggattc aaacatggcc caactaaaat
     5821 ttaactcaat cctggtggtt tgtaccggca acatttgccg ttcgcccatt ggcgagcgct
     5881 tgctgcgtaa acgtctgccg ggcgtaaaag tcaaatcggc aggtgttcat ggtctgataa
     5941 aacaccctgc agatgcgaca gcggcagatg ttgccgctaa tcatggcgta tctcttgaag
     6001 gacatgccgg acgtaagctc actgctgaga tggcacgaaa ttacgatctg attctggcaa
     6061 tggagtcgga acatattgct caggttaccg caattgctcc cgaggtccgc gggaagacaa
     6121 tgctgtttgg gcaatggctg gaacagaaag agatcccgga tccctatcgt aaaagtcagg
     6181 acgcatttga acatgtctac ggtatgttgg agcgcgccag tcaggaatgg gcgaagcgcc
     6241 tcagccgata atttgagttt ataaaatacg atgacaacta aaaatatgaa tacgccacca
     6301 ggcagcactc aggaaaatga gatcgatctg cttcgtctgg tcggcgagtt atgggatcac
     6361 cgtaagttta ttatcagcgt gaccgcgtta ttcacgctga tcgctgtcgc ttactcgctg
     6421 ttaagcacac caatttatca ggcagatact ctggtccagg ttgagcaaaa acagggcaac
     6481 gccattctca gcggcctgag cgatatgatc cctaactcat cgcccgagtc tgcaccggag
     6541 atccaactgc tgcaatcgcg catgattctc ggtaaaacca ttgctgaact gaatctgcgc
     6601 gacatagttg agcagaagta ttttccgatt gtgggtcgcg gctgggcgag attaaccaaa
     6661 gaaaaaccag gtgagctggc gatcagctgg atgcatattc cacaactgaa tggtcaggat
     6721 cagcaactga cactcacggt tggggaaaac ggccactata cactggaagg tgaagagttc
     6781 accgtcaatg gtatggtcgg ccagcgtctg gaaaaagatg gcgttgcgct gactatcgcg
     6841 gacattaagg ccaaaccagg aacacagttt gtcctgagcc agcgtaccga actggaagcg
     6901 attaacgcat tgcaggaaac ctttaccgtt agcgaacgca gtaaagaaag cgggatgctg
     6961 gaacttacca tgactggtga tgatccccag ttgattactc gtattctgaa cagcatcgct
     7021 aacaactatt tgcaacagaa tatcgctcgc caggcggcgc aggattcaca aagccttgaa
     7081 ttcttacagc gccagttacc tgaagtgcgc agcgagctgg accaggcgga agaaaaactc
     7141 aacgtttatc gccagcagcg cgattcggtt gaccttaacc tggaagccaa agccgttctt
     7201 gagcagattg tgaacgttga taatcaactc aatgagctga ccttccgcga ggcagagatc
     7261 tcccagctgt ataagaaaga tcacccaact tatcgtgcgc tgctggaaaa acgccagacg
     7321 ctggagcaag aacgcaaacg cctgaataag cgggtatcgg caatgccttc cacccaacag
     7381 gaagtgttgc gtttaagtcg tgacgtagaa gcgggccgtg cggtatatct gcaattactt
     7441 aaccgccagc aggagttgag tatttcgaaa tccagtgcca ttggtaacgt gcggattatc
     7501 gacccggcag tcactcagcc gcagccagtg aaaccgaaaa aagcgttgaa tgtggtgctt
     7561 ggttttattc ttggcctgtt tatttctgtg ggtgccgtgc tggcgcgtgc gatgttgcgt
     7621 cgtggtgtag aagccccgga acaactggaa gagcacggca tcagcgttta tgccactatc
     7681 ccaatgtccg agtggctgga taaacgcacc cgtctgcgta agaaaaattt attttctaat
     7741 cagcagcgcc atcgtactaa aaatatcccc ttcctggcgg tggataaccc ggcggattct
     7801 gctgtggaag ccgtacgtgc gctacgaacc agtctgcatt tcgctatgat ggagacggag
     7861 aataacattc tgatgatcac cggtgcgacg ccagacagtg gtaaaacgtt tgtcagttca
     7921 actctggcag cggtgatcgc ccagtccgat caaaaagtgt tatttattga tgccgactta
     7981 cgccgtggtt attcgcataa cctgtttacc gtgagtaatg aacatggctt gtcggaatat
     8041 ctggcaggta aagatgagct caacaaagtg atccagcatt ttggcaaagg aggctttgat
     8101 gtgattactc gcggtcaggt gccacctaac ccgtctgaac tgctgatgcg cgatcggatg
     8161 cgtcaattac tggaatgggc gaacgaccat tacgatctgg tgattgtcga tacgccgccg
     8221 atgctggcgg tgagtgatgc cgcggtcgtg gggcgttctg ttggcaccag cctgctggtt
     8281 gcgcgttttg gcttgaacac cgccaaagag gtgagtttgt caatgcagcg tctggaacag
     8341 gcaggcgtca atattaaagg cgctatcctc aatggtgtga ttaaacgcgc cagcaccgct
     8401 tacagttacg gctataacta ttacggttat agttactccg agaaagagta aaactgaaag
     8461 tggccggatg cggcgtgaac gccttttccg gtctacgcat tagacagttc ttcgtttgtc
     8521 atcagcctca gagcattcag gtaactgaat gctctttttt atgcattaca aactgcacgc
     8581 cggtatgcgt gcttcattca cgatttgcgt aaaacctgcc aacgaacaca tgccctgcgc
     8641 atttcgctct tcacatcctg ccagggtcag tttcacctct ccgggcggcg tatttaatga
     8701 cagcggcgtt ttatcacgca tctgctgtaa agtctggaag accagcgaaa cctgaatcca
     8761 ctggctgtta tcgcttagcc gacgccagcg ttcaaacacc agttcaccac ctggcggcgt
     8821 gttatccggc tgaccgggaa gcgtccagtt gagctccagt gcgccgccga gatttgccag
     8881 attagtatcg tgtccggcga taaacagcac tgaagtgggt aatgtcacac catacgcctg
     8941 tttttgcggt ggatggggcg tcaacgctgt cttgatcaaa tctaataacg gggtggcgcg
     9001 gctgcgggca acctctggcg tgcgttgtag caaataaaat tgcgcgttat gcaaacttag
     9061 caaggtgttc cactggtgtg aatcggtgat ccttccccac cccggctccg gcattccctg
     9121 tgcttgttgc aggagaaata tctccgtcag cattgatgcg aggcttaccg caccggttaa
     9181 tgagacattg tcggcgctca ccttgagttc cgatggtaat gcctgcgtta atgaacagct
     9241 ttcgtcctgt ttctcacgtt taaggcacaa gtttgattgc ggaaaattaa gcacccgttc
     9301 cagttcgcga aacgccgttt gccgatgccc ggtaaagtca gcaattgacc ctcctgccct
     9361 gctgaggatc gcgtcagtca cgttcgcgtt atccagttgg caaacgccag tttttagagg
     9421 attaaataac ggatcgggac tggacgtatc agcctgggta tgtacggtta ttgcacagtc
     9481 aggtgccagc ccggcggcga aggcttcgcc tgttttacgg gtacgctcgt cgacatcagc
     9541 aataatcgcg acctgaccag actgcgggca gccctttttc gccagcaatc cgtcggctac
     9601 cagacgctgg cgttggtaat gtccgagata ggcgattagc tcaccaccgc gcggtgtcag
     9661 ccaacccagt tttaccggcc aggttggcca tgcgtctggg gtgacatcct gcatcagttg
     9721 cgtggccttg gttggagcac gcacaccatg acgactgaca atcaccacac tttccagctt
     9781 cagctccggc tcactctgag cgaatgcaga ttgcggggtt aacggaatca gaagagataa
     9841 aaatgggatt aagatcgctt tcatcgatat gtttccgctt ttcatatctg acattattga
     9901 ttgcctgatg cgatgctaat gcgccgcatc aggcctacgt tcgttaactt ttcagacgcg
     9961 ggtccagcca taccaacaca agggtggaga gcgaacacat caacaaaatg ccgacgaacc
    10021 aaagtaaata ccacattgtt cctgctcctt agtacaactc gttttcgtta cggcggagag
    10081 tttctgttgt catgcgtccc cacattttgt agtagctcca gagagtgtag agcaacacaa
    10141 tgggcaaaaa tatcagcacg attaccaaca taatgctcag cgtcagctga ctggaagtgc
    10201 tgtcccacaa cgtcaggctg gagattggac tcacgcttga cggcatgaca aaggggaaca
    10261 gcgtgatgcc tgccgtgaaa atcacgccaa attgcatcaa tgacgccatt aaaaatcccc
    10321 aacccgggcg accacgataa atcgccatca ccgtcagcaa tgggcagaag aaccccagca
    10381 gcgggaagat ccacaaaacg ggcgattcga cgaaattatt catccaggca ccaggtagca
    10441 ctgccaccag tttcattaac ggattggaag gaccgttagc atcctgggcg agcagtacaa
    10501 agccatcaat accgacccac agccagtaac ccgccagcaa aaagcacagc atcaccaaca
    10561 gtgcagcgcg tttggtggcc agctgtgaac gcagatgaat cacaccaaca gttttcagtt
    10621 gtaaccagac gccaccttgc aaaatcacca tcccaaggct gagcaatccg cacaataaag
    10681 ggaatggcgt cagcagttgc cagaagctgc cgagatactc cacgcgtaat tgcggtgtga
    10741 aggcaaacgg cacgccgagc aacaagttgc cgaaggcgat accgaagact accggcggca
    10801 ccagactgcc gatgaccaga ccggcgtccc acattttacg ccagcgtgca tcggcgattt
    10861 ttccgcgata atcaaaggcc agcgggcgga agaacagtga gcacagcacc aggatcatcg
    10921 ccacataaaa gccggaaaac gccgctgcat acactctggg ccaggcggca aataatgccc
    10981 caccagcgag gatcaaccag acctggttgc cttcccagtg tgcaccaacg ctgtttatca
    11041 ctatccggcg ttcatcatca ttacgcgcca ccagcggcag cagacagccg atccccatgt
    11101 caaatccgtc ggagatcata aagaccacca ggatcacgcc aatcagcagc caccagatga
    11161 agcgcaatgt ttcataatca aacatgattt tcccctttac ccctgttgct gcgtcggttg
    11221 ttcactctgc atcgcgctcg gccccagacg ggcatatttc tgcatcaggt agacttcggc
    11281 gattaagaac agggtgtaaa gccctacgat catgatcagt gagaaagcca gttgtcctgt
    11341 ggttaaagcg gagtgcgcgg agtatgtcgg taagatgtcc tgtatcgccc acggctgacg
    11401 accaaactcg gtcataaacc acccggcttc aatcgcaatc cacggcaacg gcaaactcca
    11461 gagcgccatt ttcagcaccc agcgatgctg gtcgattttg ccacgcagcg tctggacaag
    11521 cgcaatcagc atcaccagta gcggcaggga accacagccc accatgatgc ggaaactcca
    11581 gaataccggc gcaacctgag gtatcgcgcc acgcatcgcc gcctggtact gtgcggctgt
    11641 gacatgattc atatccggcg catagcggga gagcaacatg ccgtagccca ggtcaccttc
    11701 cagttcccgg aatgcctgca acacatgcgg ctcacgattg ccttgcgata tttcctgcat
    11761 taacagccag gccatacgtc cgcgttgcaa gcgtgggtag gtttcagcca tcaaattttt
    11821 cagacccggc acggttttat ctaatgagtg agtggcgagg atccctaaca gcgcgggaat
    11881 tttgatggca aaggcgttac gctcttgatc ctgttccggc caggcaacca catggaacgg
    11941 tgcaggagca ggttccgttt gccactcccc ttccatcgcc gccagtttta ccggttgtac
    12001 ttgcgcgact tcatacgcag aactgtctcc gagttgtagc gtgccgataa tcgccagagt
    12061 accgaagacg gaaccgatgg caaacgagcg taatgcaaca tcgcgctccc gtccgcgcag
    12121 taaataccag gcgctgatcg ccataataaa catggccccg gtcacgtagc ccgccattac
    12181 ggtgtgcaca aatttcacct ggctgaccgg gttaaagacc agctcgctga agctggtcat
    12241 ctccatacgt agggtgtcga tatcaaaatg cgcaccggtc gggtattgca tccaaccgtt
    12301 ggcattcaat atccacaatg cagagagatt tgaaccgaac gccaccagcc acgtcaccag
    12361 caggtgctgg tatttattca gccgctgcca gccgaagaag aaaagcccga caaaggtgga
    12421 ttcgaggaag aaggccatta atgcttccat cgccagcggt gcgccaaaaa tatcgcccac
    12481 atagttggaa tagaatgacc agttagtacc aaactgaaac tccatggtca ggccggtagc
    12541 cacgccaaga gcaaagttga taccgaagag cttaccccag aagcgcgtca tatcgcggta
    12601 gattgttttg ccggtgacca cgtaaatggt ttccatgata gccagcaaaa aaatcagccc
    12661 cagggtaagg ggtacaaata aaaagtgata cagcgcggtc agagcaaact gccagcgcga
    12721 taaatcaatg acatcccaca tgcgcactcc tgtaggcgaa gagagaaaaa taacccgata
    12781 atgttagatc tccctcttta tcctgaaact ctacgaccag aggtattcgc cgattgcgat
    12841 attcataaga ctgaaagcga tacttaccgt ctttttacgt cggtgcagct tccgccagcc
    12901 actgacatac ctcgctaagc cgctgcgcag agtcgaccaa atcttctggc gctgccagca
    12961 ccacttccgg tattgggcag atttcataac tttccagtaa aggacctttt aaggtgtccg
    13021 tacagcgtaa atgccagaca tggcgtaacc ccgtggcgtt gatatagctc tcgccatagc
    13081 caatggtacg aatctgaata tttcccggcc cacagagacg tgagagaaaa agccgatcag
    13141 cctcgctgat gggtaattgc gtcagattaa tgctgtgcgg ctgcgcgtcg gggttacgta
    13201 catgcgccag taactcatgc gccaacggta ggccattcat caggccatca atgggcggcg
    13261 gtaacagcga atctgtcggc aagagatttt gcgtggccgc ctgccacaac gccagcggcg
    13321 cgcagccggc ctccagtttg tcctccagca acttttcgcc gcgacgtttg cgcacccgcc
    13381 ataatccgca gaagatcgcc tcctgtattt cactttcgct gtcgtcagcc tgctgaatgc
    13441 gcactgacac ttccccttcc ccgagaagcg tattgataaa gtgacgatcg tcggcgttga
    13501 gatttaacag atcgactgcc agcacctcgc ccaacggcgg ctggcgttga cttagttgat
    13561 gttgtaactc gtttaacagc gcaatcacct gcgggctgtg agcacattgc tccagggccg
    13621 ccatactcgg ttcatcattc acctgacagc tgatcggcag tggattcatg ctgaaactgt
    13681 catcgttcgg ttgcgttcct ggccccagca gatggaaaaa agtttcgctc atgaggcacg
    13741 ctcctgttgt ggttcgacaa gcccacgcat caggtttatc agttccgctc acgggtgaat
    13801 accattcagc acaccgcgat agtttccgcc ggtaaacact aatgtggcgg gaaagcgaaa
    13861 gacgccaaag cgatctccga tggcttcgct ctgttcaagg tcagcaatcg ccacctgcca
    13921 tgtgtagccg ggaaactcgc gcagtaattc gccaatcatt accggattat cgctgacctc
    13981 tggcgtgcgt ttcgggtcac tgcttaataa caccacgccg tctggcgctt gcgtaagcca
    14041 gtcgtcaaga cggaattcac taaccggcgt ccagccgcgc gccagcattc gttgccacaa
    14101 cgcatcaaat ggcgtgtcgt tgctcatccc tgctcctcca gtgtcatcct gtactggcgc
    14161 aagcgaacgc cttcgtaatt ttccatcgac agacagtcat aattgagaca gcgcgattca
    14221 ttagccggtt gcggcacaat tccccatgca gccagctgcg ccagcgccgc ctgttccgct
    14281 gcgggcagtt gctcccgtgc cagttcgctc aggctgccgc cgtagtcgtc gagcattgcg
    14341 ggttgcagac cgacgagggc aatatgtgct ggcagatgtc cgcggatatc tgccagcgcc
    14401 agcacttcgg agaaactgtt ctggtgcagg ctcattttct tcgcgctgag ataagccgga
    14461 atacgttctc cggcataggt tcgcagcgtt ccaggttcca gcccgtagtc aatggcatcg
    14521 agaatcaaca gatggctggc gctttcgaca taccccagca agttcagtcc ctgagtaccg
    14581 ccatcgacaa tctccacata ctcgggccag tggtaatggg catacaaccg ttccgccacc
    14641 cgcacgccga aaccttcatc ggcccacagc aggttgccca gccccattac cacaacgcgt
    14701 tgctcgctca tgaacgctcc ttgttactta ttttgccaaa tttgtggcta cggtagccgt
    14761 tgaccatggt ggagatcacc gtgtcgtcgg acatgatgtc ttcacgcagc gccatataga
    14821 catgaccgat cacaaacgcg ccaatcagcc acatccccag ccgatgccag ctgtgaatgt
    14881 ccattgagtt gcctcccgtc cagtagaaaa attccaccac ataacggaac ggcgcaaaaa
    14941 tagcgtactg gctgtgttcg ctgtacagcg caaagccggt gatgatcata aagaccgaca
    15001 tcaggaaata gccgaacatc gccgcctggg cgatggggtt atggcctata tcggcactcg
    15061 gacgttttgc cagaacagat accagcggat ttcataccac acgccctgcc accagctttt
    15121 acgccatacc ggcacgataa acagctcgcg ggagtatcga ttgccaacaa aagcccagta
    15181 gatccgcatc agcaagacca cggtaaaaac catcccggcg ctgaagtgaa ttaacctgat
    15241 gtagcccata tagaacagat acgtcgcctc gccgctgacg gaaggtagcg gcttgccgat
    15301 aaagtatccg gtgaccatca acaccgccat gcataacacc gtcagccagt gccagatgcg
    15361 gactggcgct tcaaagacat agtggctgac aacgttgtcg cttttctgtt gcatgatgat
    15421 tctccttcgc tgttaacgca cctgcacgga gatcagctcg ctaccgtcgt cgcccagcac
    15481 gtgtgttgaa caggcgaggc acgggtcaaa gctgtgcaga gtacgcagga tctccagcgg
    15541 ttgctcgggg atcgccattt tggtgttcat cagcgccgct tcataagcgc caatctgccc
    15601 tttgggatcg cgcgggctgg tgttccaggt ggtcggcacc acgcactggt agagatcaat
    15661 cttgccatcg cgaatggcgg cccagtggcc taacgccccg cgcggcgctt cggtaaagcc
    15721 gacgccacgg cactctgtcg gccaggttgc aggttcccat ttttccgtgg aggcggtggc
    15781 aagattgccg tttttcaggt tagtcatcag cttgttgaag aaatattgca acttacctgc
    15841 ggcccactgt gcttcgtgcg cgcggcacaa aatgcggccc aacgttgact ggataccgga
    15901 aagcggcagg ttcagcgccg acatcatgcg atcgaccgac tcaacggtcg cagcatcgcc
    15961 tttgtgataa gcgattaacg tgcgtgccag cggccccact tccatcgcgt taccgcgcca
    16021 gcgcggcgct ttgatccacg agtagcgttc ctgttcattc agctgctgaa tgttggtatc
    16081 gctgcctttg acatcgccgg ggttgtacca cgggtcggta atgccatcga atggatgacg
    16141 cccgacctga tcgttgggat aacgatacca ggcgtggtcg acaaactcct gcacctgctg
    16201 cggatcaacc aaatccactg gcagcacatt gttgaagtcg ccgttaatca ccgcgccgcc
    16261 aggcatcagc agacttttct caccaaagtc gttggcaata tccgggaacg caccgtagct
    16321 gaggacacat ttatcagaaa gacccgtgcc gatttcgctc cacggtttgt tgaactgacc
    16381 gatggctaag gcgtcgggga tcatcacgtt gttaatgaag tccgccgtgc gggtgataat
    16441 cgactgcacc aggttcaggc gttccatatt gaccgccccg accgcgccgc tttcgtcaat
    16501 gttaatggcg caaggcatcc cgccgacaat ccagtttgga tgcgggtttt taccgccaaa
    16561 gaccgcgtgg attttgacaa tttcacgctg gaaatcgaga gcttcgagat agtgggcaaa
    16621 gcccatcagg ttagcttctg gcggcagttt gtactgcggg tgcccccagt agccattgcg
    16681 gaagatcccc aactgcccgc cttcaacaaa ttttttcagg cggttttgta cgtcgaagaa
    16741 atagccaggg gatgatttcg gccaagagga gagactttgc gccagttcgg aggttttccg
    16801 cgggtcggct ttcagcgcat ctaacacatc gatccagtcc atcccggcaa gctgatagaa
    16861 gtgcaccaga tgatcgtggc accagagcgt tgccagcata atgttgcgga tgatattggc
    16921 gttgtccggc actttaatac cgatagcatc ttcgatggcg taaaccgaag ccagggcgtg
    16981 tacgccagta cagacgccgc agatacgttc aacgaacgcc cacgcatcgc gcgggtcgcg
    17041 cccttgtagg atgatctcca gcccgcgaaa catggtgccg caggagacgg cattggtgat
    17101 cacattctga tcgttaatat tcacttcgca gcgcatgtgg ccttcgatgc gcgtaatcgg
    17161 gtcgaccacc aggcggcgtc cggcattatt gatggtgtat ccctgagttt cgtactgagt
    17221 gctcatgcct gtttatcctc attgcctggc tgatgttcgg tttctgtagg ttgctggtta
    17281 tgacgtctgc gctggtcaac ggcgctggcg actgcgtgca caccaacagc cgctgccacc
    17341 acaccaagcg cggttaaacc gacggtatcg gcggtggaat gagtacccat ttgcggaata
    17401 tcgaccacgc ggctgtagaa cgaaccgcaa tcccagaaac cattttccgc acagcccagg
    17461 cagccgtgac cagactggat tgggaaagaa acgccatcat tccagcgtgt ggaggaacag
    17521 gcgttatagg tggtaggccc tttgcagccc attttgtaca ggcagtaacc tttgcgggca
    17581 gcgtcatcat cccaactctg gacgaactct ccggcgtcga agtgggcgcg gcgatagcat
    17641 ttatcgtgga ttcgctgacc atagaacatc agcggacggc ccattctgtc gacatctggc
    17701 aagcgatcaa aggtcaccat gtaagtaatg atggcgctca tcacatccgg gatcggcggg
    17761 cagccaggta ctttgataat gggtttgtcg gtgatgactt tgtcgatagg cgttgcctgc
    17821 gtcggattgg gccacgcggc ctgcacgcag ccccaggacg cgcaggttcc ccaggcgata
    17881 atcgcgctgg ctccggcagc ggcacgtttg agtttctcaa taaacggtcg accgctgctg
    17941 atacagaaca tcccctgctc gcccagcggc ggattacctt ctactgcgag gatatatttg
    18001 ccattgtatt gcgtgatgat gtcttcgaag acttcttccg cctgggttcc ggcggcagcc
    18061 atcaaagtat cgtcataatc gagggaaatc agggaaagga tgacgtcctt cgccagtggg
    18121 tgagcggagc ggataaaaga ttcggtacag caggtgcatt ccagaccgtg gatccatacc
    18181 accggaatgc ggggtttgtt ctccagcgcc caggcaatct ttggtgccat tcccgcgcct
    18241 aatcccagcg acgtggcagc cagactacaa tatttgagaa agctgcgccg ggtaacgccc
    18301 tgacgccgca tggcctggta aaatgtttcc tcgttattca tatcgcacgt ctcttctcct
    18361 tgcgacaccg gcaggacacc gggcgcttat ggtcggcaag cgacgtccct gtagcaaggc
    18421 aaaccatcgc ttcacgtgag atgctgaaaa cgaaagctca tccttctgca ctggcgcacg
    18481 tcgccagaaa gtattgttaa taaagcgtag tgaaactt
//
LOCUS       DADMKF010000074        18214 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.075, whole genome shotgun sequence.
ACCESSION   DADMKF010000074 DADMKF010000000
VERSION     DADMKF010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18214
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.075"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            15..1091
                     /locus_tag="JNC24_002361"
     CDS             15..1091
                     /locus_tag="JNC24_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAY9159091.1"
                     /translation="MQRKKYDPNLPRNLTYRRRDKAYYWRNPLTKEEFTLGKISRRDA
                     VTQAIEANHYIYKNYSPAALIEKLKGFDSFTMADWIERYKTILIRRKVSRNTYKIRAN
                     QLETIKEKLGEILLTEITTRHIAEFLDLWIEGGKNTMAGSMRSVLSDMFREAIVEGRI
                     SQNPVTPTRAPKIVVTRERLKLKIYNCIREAADQLPAWFPLAMDLALVTGQRREDITN
                     MRFSDIYDDRLHVRQIKTGMMIAIPLSLSLPVAGLRLGTVVERCRLVSRGDYLISAGI
                     RKNSPDGSIHPDGLTKKFVAARKFTGIQFSENPPTFHEIRSLAGRLYKETCGEEFAQR
                     LLGHTSEKTTKMYLDEREKTYLLL"
     gene            complement(1484..1825)
                     /locus_tag="JNC24_002362"
     CDS             complement(1484..1825)
                     /locus_tag="JNC24_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HAY9159092.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTNAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(1838..2710)
                     /gene="copD"
                     /locus_tag="JNC24_002363"
     CDS             complement(1838..2710)
                     /gene="copD"
                     /locus_tag="JNC24_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
                     /protein_id="HAY9159093.1"
                     /translation="MLAFTWIALRFIHFTSLMLVFGFAMYGAWLAPLTIRRLLAKRFL
                     RLQQHAAVWSLISATAMLAVQGGLMGTGWTDVFSPNIWQAVLQTQFGGVWLWQIVLAL
                     VTLIVALMQPRNMPRLLFMLTTAQFILLAGMGHATLNEGVTAKIHQTNHAIHLICAAA
                     WFGGLLPVLWCMQLIKGRWRHQAIQALMCFSWCGHFAVIGVLASGVLNALLITGFPPT
                     LTTYWGQLLLLKAILVMIMVVIALANRYVLVPRMRQEEDRAAPWFVWMTKLEWAIGAV
                     VLVIISLLATLEPF"
     gene            complement(2714..3088)
                     /gene="yobA"
                     /locus_tag="JNC24_002364"
     CDS             complement(2714..3088)
                     /gene="yobA"
                     /locus_tag="JNC24_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HAY9159094.1"
                     /translation="MASTARSLRYALAILTTSLVTPFVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            3227..3457
                     /locus_tag="JNC24_002365"
     CDS             3227..3457
                     /locus_tag="JNC24_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HAY9159095.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            3559..4215
                     /locus_tag="JNC24_002366"
     CDS             3559..4215
                     /locus_tag="JNC24_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HAY9159096.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVRAAARQQCQLLVFPS
                     LSLLGCDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIRGIAVFAPW
                     RKTPGIYHQSHGACLGRRSRTITVVDEQPEGMDMDPTCSLFTTGQCLGEPDLLASARR
                     LQFFSHQYSIAVLMANARGNSALWDEYGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            4239..4901
                     /gene="exoX"
                     /locus_tag="JNC24_002367"
     CDS             4239..4901
                     /gene="exoX"
                     /locus_tag="JNC24_002367"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HAY9159097.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSHLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGIKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERDPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(4898..6958)
                     /gene="ptrB"
                     /locus_tag="JNC24_002368"
     CDS             complement(4898..6958)
                     /gene="ptrB"
                     /locus_tag="JNC24_002368"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HAY9159098.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWEILLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDKLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPCENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTDERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDNHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPAD"
     gene            complement(7167..7826)
                     /locus_tag="JNC24_002369"
     CDS             complement(7167..7826)
                     /locus_tag="JNC24_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HAY9159099.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDAKERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVRDGIERDLEQQKRTLA
                     E"
     gene            complement(8153..8509)
                     /gene="yebF"
                     /locus_tag="JNC24_002370"
     CDS             complement(8153..8509)
                     /gene="yebF"
                     /locus_tag="JNC24_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HAY9159100.1"
                     /translation="MKKRGAFLGLLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            complement(8576..8866)
                     /gene="yebG"
                     /locus_tag="JNC24_002371"
     CDS             complement(8576..8866)
                     /gene="yebG"
                     /locus_tag="JNC24_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
                     /protein_id="HAY9159101.1"
                     /translation="MAVEVKYVVIREGEEKMSFTSKKEADAYDKMLDTADLLDTWLTN
                     SPVQMEDEQREALSLWLAEQKDVLSTILKTGKLPSPQVVGAESEEEDASHAA"
     gene            9000..10178
                     /gene="purT"
                     /locus_tag="JNC24_002372"
     CDS             9000..10178
                     /gene="purT"
                     /locus_tag="JNC24_002372"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HAY9159102.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSESLFREAVADIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWKYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRRLGVALATAESVVDAIERAKHAAGQVKVQG
                     "
     gene            complement(10234..10875)
                     /locus_tag="JNC24_002373"
     CDS             complement(10234..10875)
                     /locus_tag="JNC24_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAY9159103.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(10912..12723)
                     /gene="edd"
                     /locus_tag="JNC24_002374"
     CDS             complement(10912..12723)
                     /gene="edd"
                     /locus_tag="JNC24_002374"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020244583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HAY9159104.1"
                     /translation="MNPQLLCVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSLGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEQ
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            complement(12958..14433)
                     /gene="zwf"
                     /locus_tag="JNC24_002375"
     CDS             complement(12958..14433)
                     /gene="zwf"
                     /locus_tag="JNC24_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HAY9159105.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFNRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            14771..15640
                     /locus_tag="JNC24_002376"
     CDS             14771..15640
                     /locus_tag="JNC24_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAY9159106.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAAMALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRTVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            15768..17210
                     /locus_tag="JNC24_002377"
     CDS             15768..17210
                     /locus_tag="JNC24_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase II"
                     /protein_id="HAY9159107.1"
                     /translation="MSRRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(17270..18064)
                     /locus_tag="JNC24_002378"
     CDS             complement(17270..18064)
                     /locus_tag="JNC24_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005138488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NGG1p interacting factor NIF3"
                     /protein_id="HAY9159108.1"
                     /translation="MVAVSQPTVDKIIFGAEATLINKIGTCWIASMDVLRKAVFEGVN
                     IIITHEPTFYSYADLEGDDLEFSWARKIMDYTRGELSYLKIIEQKKEFLHKNNLVIIR
                     CHDVMDREPTFGMSKALAQQLELDVTNIVASDEMYCVYAIDPDSAINITKRFAKNLKI
                     YNLPGIQFYGDKARVVRTVGIGAGCFCDPIQYMEYQADYYITINDSIKTWVQTQYSKD
                     SGLPMAVIGHSVAEEAGMRRLASYLDLHSGYPCIHFTGGCDYDWIE"
BASE COUNT         4672 a         4575 c         4391 g         4576 t
ORIGIN      
        1 ggagaagtta aaacatgcag cggaaaaaat acgatcccaa tttgcctaga aacttaacat
       61 atcgaaggag agacaaagca tattactggc gcaaccctct gacgaaagaa gaatttacac
      121 taggtaaaat ttcaagaaga gatgcagtaa cgcaggcaat tgaagcaaat cattatatat
      181 acaaaaacta ctctcccgct gccttaattg aaaaacttaa agggttcgac tcatttacta
      241 tggcagactg gattgaacgt tacaaaacga ttcttataag gagaaaagtg tccagaaata
      301 cttataaaat tcgggcaaat caactggaga caataaaaga aaaattggga gagattttac
      361 tgacagaaat aaccactcgc catattgccg agtttcttga tttgtggatt gaaggaggga
      421 aaaacacaat ggcaggatca atgcgttctg tgttgtctga tatgtttcgc gaggccattg
      481 ttgaaggacg tatatctcaa aatccagtca cgccaacaag agcaccgaaa atagtagtta
      541 caagagaacg actgaaacta aagatataca actgcatcag ggaggcagca gatcaacttc
      601 cggcatggtt cccattagct atggatttag cccttgtaac aggacaacgt cgcgaagaca
      661 taacgaatat gcggttcagt gatatttatg atgatcgtct ccacgtcagg caaattaaga
      721 caggaatgat gattgctatc cccctgtcac tcagccttcc tgtcgctggt ctacggcttg
      781 gtacagtagt tgaacgatgc cgcctggtaa gccggggaga ttatctaatc agtgccggga
      841 ttagaaaaaa cagccctgac ggcagcattc acccggatgg cctgacaaaa aaatttgtcg
      901 cagccagaaa attcacaggt atccagttca gtgaaaaccc accaactttt cacgagatca
      961 gaagcctggc tggacgattg tacaaagaaa catgtggaga agaatttgct cagcgtctac
     1021 ttggccacac atcggagaag acaacaaaaa tgtatcttga tgagagagaa aaaacgtact
     1081 tactgctctg attttaacgt aaatggattg ttaaatgtat tttggttgtg atataaccaa
     1141 aaagaccgga atacagaaat tcgagtaaat ttcggggaat ttcgggggag acatttgcaa
     1201 ctaattgatt tcaaatgcaa ttaaaaaaag accgaatacg attcctgtat tcggtccagg
     1261 gaaatggctc ttgggagaga gccgtgcgct aaaagttggc attaatgcag gcttagttgc
     1321 cttgcccttt aagaatagat gacgacgcca ggttttccag tttgcgtgca aaatggtcaa
     1381 taaaaagcgc ggtggtcatc agcttaaatg ttaaaaaccg cccgttctgg tgaaagaact
     1441 gaggcggttt ttttattgga aatcaaaagg ctattttagg taattaacag agtttttcag
     1501 ctcgttctat aaacggtgcc agactcattt tttcgccggg attgttagga tcatcaatct
     1561 gaatcaccga aatgggttgg gcattggtct tcccactggc aacttccttt tgtgcgatat
     1621 cgtttaaagg atactgcacg agggtactcg gattaataac atacaaagca ttacccggtc
     1681 ggcaagtcag catcacctct tcgcgattaa acgcccattt gtctttaccc acttcaaaac
     1741 gactgacggt aatcacctgc ggtgcagcca gcgccgctgc agaactggtg agtaacagaa
     1801 acgccagaat actttttttc atcattttcg taatccatca aaaaggttcg agagtcgcaa
     1861 gcaggctgat gatcaccagc actaccgcac ctatcgccca ttcgagttta gtcatccaca
     1921 caaaccaggg tgccgcacga tcttcctcct gcctcatgcg cggtacaaga acataacgat
     1981 tagccagcgc aatgaccacc ataatcatca ccaggatggc tttgagcaat aagagttggc
     2041 cccagtaagt cgtaagcgtg gggggaaatc cggtaatcaa caatgcatta agcacgccgc
     2101 ttgccagtac gccgatcacc gcaaaatgcc cgcaccatga aaaacacatc agcgcctgaa
     2161 tagcctggtg tcgccaacgg cctttgatga gttgcataca ccagagcacc ggcagtaatc
     2221 ccccaaacca cgccgccgca caaatcaggt gaatcgcgtg attagtctga tggattttcg
     2281 ccgttacccc ttcattcagt gtcgcatgcc ccattcccgc cagcagaata aattgcgcgg
     2341 tagtgagcat aaacagcaaa cgtggcatat tccgcggttg cataagggcg acgatcaacg
     2401 ttacgagggc gagaacaatt tgccataacc agacgccacc aaactgcgtt tgcaaaacag
     2461 cctgccaaat gtttggcgaa aatacatctg tccatcccgt tcccatcaac ccgccctgaa
     2521 cggcgagcat tgcagtggca ctgatcaaac tccagacggc agcgtgctgt tgtaaacgca
     2581 gaaaacgctt cgccagcaag cgacgaatcg tcaagggggc cagccaggca ccgtacattg
     2641 cgaagccaaa aaccagcatc agcgaggtga aatggataaa tcgcagcgcg atccaggtaa
     2701 acgccagcat gatttatttc acgctaaagg tgtagtgtcc tttcgtttta tgcccgtcca
     2761 ccgaaacaac atgccagtca acggtgtagg ttccgggttt cagcgaatcg gccagtggaa
     2821 caatcagttg cttttggtcc tgctcatttc gcttcgccgg taatgtttta atattttcgt
     2881 tttttggccc cgtgattttt gcaccactga atcctgtttc cacaccttcc gagaagttta
     2941 aagtgattgc ctgcggtgca gctgtcactt gcgcgtttgc cgcaggatac tgatgcgtta
     3001 aatgcgcatg tgcccagacg aaaggtgtca ccaacgaagt ggtcaggatt gccagcgcgt
     3061 agcgaaggga gcgtgcagtt gaagccatat tatctattcc tttttgtaat aactttttta
     3121 cagagcataa ccttgtctaa tgtctgagtc gaggatcatc aattccggct tgccatcctg
     3181 gctcactctt agtaactttt gcccgcgaat gatgaggaga ttaagaatgc tgaagaatct
     3241 ggctaaactg gatcaaacag aaatggataa agtgaatgtc gatttggcgg cggccggggt
     3301 ggcatttaaa gaacgctaca atatgccggt gatcgctgaa gcggttgaac gtgaacagcc
     3361 tgaacatttg cgcagctggt ttcgcgagcg gcttattgcc caccgtttgg cttcggtcaa
     3421 tctgtcacgt ttaccttacg agcccaaact taaataaaac ttatacaggg ttacactttc
     3481 ttacataacg cctgctaaat tatgagtatt ttctaaaccg cacccataat ttgcagtcat
     3541 tttgaaaagg aagtcattat gtcgttctgg aaagttgcag cagcgcaata tgagcctcgt
     3601 aaaacctcac tcaccgaaca agtagctcat catctggagt ttgtgagggc cgccgccagg
     3661 caacaatgtc agctgttagt ttttccatcg ctttctttac tggggtgtga ttattcgcga
     3721 cgtgcccttc ctgccccacc cgatctctca ctgttagacc cgctttgtta tgccgcaacg
     3781 acctggcgaa tgaccatcat tgccggcctt cccgttgaat ataacgatcg ctttattcgt
     3841 ggaattgcgg tgttcgcacc gtggcgaaaa acgcctggga tctaccatca gagtcatggt
     3901 gcatgtctgg gccgtcgttc cagaaccatc acggtggtcg atgaacaacc ggaaggtatg
     3961 gatatggacc cgacctgttc actgtttacg acagggcaat gtctgggaga acccgacctg
     4021 ctggcgtcgg cccgtcgctt acaatttttt tcacatcagt actcgattgc tgtgctgatg
     4081 gcaaatgccc gtggtaacag tgcactatgg gatgaatatg gtcgtctcat cgttcgcgcc
     4141 gatcgtggtt cattattgtt agtcggtcag cgttcatcac agggttggca aggcgatatc
     4201 attccattac gctaggcttt ttcggcctgg agtatgccat gttgcgcatt atcgatacag
     4261 aaacctgcgg tttgcaggga gggatcgttg agattgcctc tgttgatgtc attgacggaa
     4321 aaatcgtcaa ccccatgagc cacctggtgc gccccgatcg tcctattagt ccacaagcga
     4381 tggcgattca tcgcatcacc gaagccatgg tcgccgataa accgtggatt gaagatgtga
     4441 tcccacacta ttacggcagt gaatggtatg tcgcgcacaa cgccagcttt gaccgccgcg
     4501 tactgcctga gatgcccggt gagtggattt gcaccatgaa actggcccgt cgcttgtggc
     4561 caggaatcaa gtacagcaat atggcgttat ataaaacacg gaagctcaat gtacagacgc
     4621 cgccgggcct gcatcatcac cgcgcgttgt atgactgtta tatcaccgcc gcgttgctta
     4681 tcgatattat gaacacctcc ggctggacgg cagaacagat ggccgatatc accggacgtc
     4741 cgtcgttgat gacgaccttc acctttggca aataccgtgg caaagcggtt tccgacgttg
     4801 ccgaacgcga tccgggctat ctgcgctggt tatttaataa cctggacagc atgagcccgg
     4861 agctgcgttt aacactgaaa cattatctgg aaaatactta atccgcaggc gtagcgggta
     4921 atgttccctg cgccagcgcg accagaaaag catattccat cgctacgcct tcgtacgatt
     4981 taaagcggcc agatttaccg ccatggcctg agtccatgtc ggtacagagc aataaaagat
     5041 ggttatcggt tttcagctcg cgcaatttag cgacccattt tgccggttcc caatattgca
     5101 cctgagaatc gtgtaaaccg gtcgttacca gtaaatgcgg ataagcctgt gcggtgacgt
     5161 tgtcatacgg gctgtagctt ttcatgtact cgtaatattg cggatcctgc gggttacccc
     5221 actcttcaaa ctcaccagtg gtaagaggaa ttgattcatc aagcatcgtt gttacaacat
     5281 caacaaacgg tacctgggcg ataacgccgt ggaataattc cgggcgttga ttaattgcaa
     5341 cgcccatcaa catgcccccc gcactcccgc ccatcgcata acaaagcgaa ggagagccat
     5401 agcccagttt taacaatgca tcgcaggcat caagataatc attaaacgta tttttcttct
     5461 tcagaaattt tccgtcttcg taccattgtt gccccagctc accaccgccg cgaacatgga
     5521 caatggcgta gacaaagcca cgatctaaca aactcaagcg gctaaaactg aaatcggcat
     5581 caatacttgc gccgtaagaa ccatagccat acaccagcaa cgggttgtgt cctttgcgaa
     5641 aatgtttgcg atggtagacc aacgaaaccg gaacttcgac gccatcacgg gcgactatcc
     5701 acaggtgttc actgcggtaa ttcgccgcat caaaaccagg aacttccgtt tgttttaata
     5761 cacgacgctc atcggtatcc atatccagtt caaacaaagt gtctggtgtg gtcatggaag
     5821 aataaccata acgcaatcgc gcggtttcag gttctggatt gtaggcaatc caggtcacat
     5881 aggctggatc atcaaaggca ataccaatga cttcccgggt cttgcggtta atttggcgca
     5941 aactggttaa cccgcgctga cgctcttcaa ccaccagcca gtcggtaaac agcgtaaacc
     6001 cttccagcat gatgttttcg catggcggaa ttaactcttc ccactgttgc tcatcacgca
     6061 tacgggtacg gtataagcca aagtttttgc cgtggcggtt ggaacgcaga taaaaacgat
     6121 gctggtagtg atcaaggctg tattcgtgat ctttgcggcg cggcagaaaa acaaacggct
     6181 cggcatcggc catttccgcg tccagcaggc gaacttcact ggtggtggcg ctggccaaat
     6241 gaatgactac atagtgcttc gaggtcgttt tatgcaggct gacgtaatag gtatcgtctt
     6301 tttcttcgta gatcagttta tcttgcgatg ctggcgtacc gatggcgtga cgccagacct
     6361 gataaggcag cagcgtcacc ggatgcttgc gaacatagta gaaagtccag gagtcatttg
     6421 cccagacaaa gctgggttca acgttatcca gcagttccgg gtaccagtta ccagtttcca
     6481 gattacgaaa acgaatgccg tactggcgtc gggaaagaaa atcttctgcc agcgccataa
     6541 tggtgttatc gggcgtaatc gccattccgc ccatcgaata aaactcacta tgagctgcgc
     6601 gcttattggc atcgagcaat atttcccact catcccactc ttcactgaat gccgattgac
     6661 gctggtagat agcatattca cagcctggtt cataaatatg ccgatagcgg tagccatttt
     6721 tgatgtaggg cgcagaaact tctcgttgcg gaatgcggtc gatgatttcc tttaagatgc
     6781 gatcctgcaa ggcttgttgt gaggccatca cccgatgacc gtaactattt tcttgttgca
     6841 ggtagtccag gacttctggc tgagaacgcg tatcatcccg cagccagtag taattatcga
     6901 tgcgcgtatc gccatgaagc gtcatggcgt ggggaatgcg ggcggctttt ggtagcatgt
     6961 tattgttctt tctggttgaa acatcttata agggtggcaa aactcaccgg ggatgcaagc
     7021 gaaacagggg agtcattgct tagatgatga caggtaatgg cacggatatc gaatgttatg
     7081 cagacagaga aactcagcct gttcaaatgg ctgtgcggtt ctggatagcc cgaaatagtc
     7141 aacttcaggc tatccagaga gcggaattat tccgccaaag tgcgtttttg ctgttcgaga
     7201 tcgcgttcaa tgccgtcacg aacatcctgg gggattttca gcgcgtcacc cagtgcattc
     7261 aggtaactgc gttccataaa atggtcaata tcaatagccg cgcaactcag gaaatagatt
     7321 tccagcgcct cttcttcatt gcggaccccg gtagccaggc gttgtggatc cagcggttgt
     7381 tcgattgcct gctcaatgag tacacgcccc tgctcttcca cgccggcttc acgcaattgc
     7441 tggtcgattg ccgcacgttc tttggcatca atatgaccat cacttttagc ggcaaagacc
     7501 agcgcaagga tcaaacgttc tgtacgctca tccagcggcg tactttgcgc gccaaactgc
     7561 ggttcgtcct gatgcgccgc gcgaatttta tctttgtatt tattccacag caccgtaccc
     7621 gctaccgctc cgccgccaac cagtaacgcg ttggtgccat atttcgtaag aagtttacgt
     7681 gctgatttat ttgcgaccag cagcccagcc agtccgccta atgcgcctgg cactaacagt
     7741 ttgcccaatc cctgatccgc agacgaggag gtagaagaac tgctttgccc aagcagggat
     7801 tgcagttgat ttaaccagtt agccatattt gctcctcaat aaccattttt agtgcctgta
     7861 gcatagcgga tgaggatgtc agaaaatgta tatcgagaca aaagatgcgc aaataagcgg
     7921 ggcggtaggc cgttattcaa agaaattcgc gccaggtaag gtatttactg tatatcaggt
     7981 gataagggta ttttaggtga aagtagtttg tcgttttgag tggcattgtt tgatgctgat
     8041 tgcccgatgc gacgctgttg cgtcttctca ggcctacgat tgtggtgcgt tctggggcgg
     8101 acaaggcact cgcgccgcat ccaccaacgg tcaatcgaat tatcagaagc cattaacgcc
     8161 gctgatattc cgccattccc gctttgcagt ccacgctgac ctggtaatga atatcggcac
     8221 ttttaccacg cacggttagc ggtactgacc atttatcatc tttaccctga atgtcctgca
     8281 aactgaccca agccacggga tcggcctgac cgacaatttt ttgatcatct gcccaacgcg
     8341 ccacgcgatt ttgttgataa tcacgtttta cgctcgcggc aattccggca gcatccagac
     8401 cttcacactt tgggaaagtg accgacttgc tggtttcatt attggcagcg aaaactgatg
     8461 cgcaggcaga aaccaacaac agccctaaaa acgcccctct ttttttcatg tttttctcca
     8521 tagcacaatg attcaggaga aagcatggta caaattgtca ggagcgcaag ttgcttcagg
     8581 ccgcgtgtga ggcatcttcc tcttcggatt cagcaccgac gacctgtggg gacggtaatt
     8641 tgccggtttt cagaatggtg ctcagcacat ctttttgttc tgccagccat agcgaaaggg
     8701 cttcacgttg ctcgtcttcc atttgcactg gagaatttgt cagccaggtg tcaagcagat
     8761 ccgccgtatc aagcattttg tcataagcat cggcttcctt tttgctggta aacgacattt
     8821 tctcttcgcc ctcacgaatg actacgtatt taacttcaac cgccatttgc agcctctcat
     8881 aataactgtg attttataca gtatatttct tttcggttga gaaatcaaca tcagcaataa
     8941 agacacacgc aaacgttttc gtttatactg cgcgcggaat taatcagggg atattcgtta
     9001 tgacgttatt aggcactgcg ctgcgtccgg cagcaactcg cgtgatgtta ttaggctccg
     9061 gtgaactggg taaagaagtg gcaatcgagt gtcagcgtct cggcgtagag gtgattgccg
     9121 tcgatcgcta tgccgacgca ccagccatgc atgtcgcgca tcgctcccat gtcattaata
     9181 tgcttgatgg tgatgcatta cgccgtgtgg ttgaactgga aaaaccacat tatatcgtgc
     9241 cggagatcga agctattgcc accgatatgc tgatccaact tgaagaggaa ggactgaatg
     9301 ttgtcccctg cgctcgcgca acgaaattaa cgatgaatcg cgagggtatc cgtcgcctgg
     9361 cggcagaaga gctgcagctg cccacttcca cttatcgttt tgccgatagc gaaagccttt
     9421 tccgcgaggc ggttgctgac attggctatc cctgcattgt aaaaccggtg atgagctctt
     9481 ccggcaaggg gcagacgttt attcgttctg cagagcaact tgctcaggca tggaagtacg
     9541 ctcagcaagg cggtcgcgcc ggagcaggcc gcgtaattgt tgaaggcgtc gttaagtttg
     9601 acttcgaaat taccttgcta accgtcagcg cggtggatgg cgtccatttc tgtgcaccgg
     9661 taggtcatcg ccaggaagat ggcgactacc gtgaatcctg gcaaccacag caaatgagcc
     9721 cgcttgccct tgaacgtgcg caggagattg cccgtaaagt ggtgctggca ctgggcggtt
     9781 atggtctgtt tggtgtcgag ctgtttgtct gtggtgatga ggtgattttc agtgaggtct
     9841 cccctcgtcc acatgatacc gggatggtga cgttaatttc tcaagatctt tcagagtttg
     9901 ccctgcatgt acgtgccttc ctcggacttc cggttggcgg gatccgtcag tatggtcctg
     9961 cagcttctgc cgttattctg ccacaactga ccagtcagaa tgtcacgttt gataatgtgc
    10021 agaatgccgt aggcgcagat ttgcagattc gtttatttgg taagccggaa attgatggca
    10081 gccgtcgtct gggtgttgcg ctggcgaccg ctgagagtgt tgttgacgcc attgaacgcg
    10141 cgaagcacgc cgccggacag gtaaaagtac agggttaaac ccggcaaaaa aacgctacaa
    10201 aaatgcccga tcgtagatcg ggcgttttga cttttacagc ttagcgcctt ctacagcttc
    10261 acgcgccagc ttagtaatgc ggtcgtaatc gcccgcttcc agcgcatctg ccggaaccag
    10321 ccaggaacca ccgatgcaca gcacgctttt cagcgccagg tagtcacggt agttagccgg
    10381 agaaatacca cccgtcgggc agaaacggac ctgggagaac ggacccgcga tcgcctgcag
    10441 ggctttcacg ccgccgttag cttcagccgg gaagaatttg aactctttca aaccgtagtc
    10501 catacccagc atcagttcgg aaacagtgct gatccccgga attagaggaa tagtcccttc
    10561 ggtagcagct ttcagcagcg gctcggtcag acccgggcta attgcgaact gtgcacccgc
    10621 ttcagtgact tctgccagct gctgtggatt cagcaccgta ccggcaccca caatcgcttc
    10681 aggcacttct ttggcgatag cacggatagc gtcaactgca cactcggtac gcagagtcac
    10741 ttccagaacg cgcaccccac cagcaaccaa cgcttttgcc atcggcactg cgtgttccag
    10801 tttttttacc acgataaccg gtacaaccgg gccggtggtc aggattgatt ctgcacttgt
    10861 tttccagttt ttcatcagag ttttctctcg cctgattaca aatttgtcgt cttaaaaagt
    10921 gatacaggtt gcgccctgtt cggcaccgga cagtttttca cgcaaggcgc tgaataattc
    10981 acgtcctgtc cccacgcgtg acgcgctcag gtcaggaatg tgcggttcgc gagcagccag
    11041 ttccgcttcg tctaccagca gcgtcagttc gcctgtctgt ccattcacac gaatgatgtc
    11101 cccgtcgcgc acttttgcca gcagcccgcc atcgtaggct tctggtgtta cgtggatagc
    11161 tgacggcact ttacctgaag cgccggagag tcgtccatcg gtaactaacg caattttgaa
    11221 acaccggtcc aataatacac caagtggcgg catgagttta tgtaattctg gcattccgtt
    11281 cgcttttggc ccctgatgac ggacaacaac gacacaatcg cggtccagca aacccgcttc
    11341 aaaggccggc ataacgtcat gctggctttc aaaaacaacc gctggcgctt caatcacctg
    11401 gttctcaacc ggcacggcag aggttttcat aaccgcacgg cccaggttac cgcttaacac
    11461 ttttgtccca ccatgatgag agaaaggttg ttcgaaggaa gcgatcacat tgctgtcgag
    11521 tgatttttcc gccccttccc gccagtcctg ttcaccatta ttcagccatg gttcaagggt
    11581 ataacgagac agaccaaagc ctgccaccgt attgacatct tcatgcagca ggcctgcttt
    11641 gagcagttca cgcaccagaa ccggtacgcc acctgccgcc tggaagtggt taatatcggc
    11701 cggaccgttc gggtagagac gtgccatcag cggtacaaca tcagaaaggt cagagaagtc
    11761 atcccagtta atctgaatac cggccgcgcg cgccatcgcc accaggtgca tggtgtggtt
    11821 agtggaacca ccggtcgcca gcagtgcaac gataccgttc accaccactt tctcatcgat
    11881 catcttaccg atcggcatcc attcattacc attaccggtc atgcgtgtaa cctgacgcgc
    11941 agcggcagcg gtcaaagcat cgcgcagcgg agaatccgga tgaacaaaag aagagcctgg
    12001 caactgcatc cccataaatt ccaccaccat ctggttggtg ttggcagtac cgtagaaagt
    12061 acatgttccc ggcgcatggt aagacgcggc ttctgactcc agtaaggcca tgcggtccac
    12121 tttaccttcg gcataaagct ggcgaatacg cactttttct ttatttggca aaccgcttgc
    12181 catcggtcca gacggcacaa acaccgcagg caaatgacca agcgacaggg ctgccatcgt
    12241 cagacccggg acaatcttgt cgcacacacc gaggaacaga gcaccatcaa acatgttatg
    12301 ggacagcccc accgccgcag acatcgctat cacttcgcgg cttagcagcg acaattccat
    12361 tccatcctgc ccctgggtga caccatcaca catcgccgga acaccgcccg caacctgacc
    12421 aaccgcattc gcttcatgca gggctttacg aatgatttct ggatagtgtt cataaggctg
    12481 gtgcgcggag agcatgtcgt tataggaggt gatgatggcg atattgttac gcaacatgct
    12541 tttcaaagag gctttgtctt ctggctggca ggcagcgaaa ccgtgtgcca ggttaccgca
    12601 tgccaactgc gaacgatgaa cggtcgacgt tttcgcttgt tctatccggg cgagataagc
    12661 agagcgagtc tcgcgcgaac gttcaatgat tcgatttgtt acgcataaca attgtggatt
    12721 cataaaggct cctgaaattg agttgtcaga gcaggatgat tcacaacgcg tttcattcag
    12781 aggatttatg agtgaaacgc ctgtaaccgg agctcatagg gcaaaaacgt ttcagtcagt
    12841 gtaataaaaa aagcctcgtg ggtgaatccg cacgaggcct gaaagtgtaa aaattgttct
    12901 acaatctgtg caagatcatg ttaccggtaa aataaccata aaggataagc gcagatatta
    12961 ctcaaactca ttccaggaac gaccatcacg ggtaatcatc gccaccgagg caacgggtcc
    13021 ccaggttccg gcctgatacg gtttcggcgc atcattgtcc atcgcccacg cctcagtaat
    13081 ggagtctacc catttccagg cttcttccac ttcatcacga cgtacaaaca gtgcctgaat
    13141 accacgcatg gtttccagca gcagacgttc ataggcatcc gccagatgcg tctgattaaa
    13201 ggtttctgaa tagctcagat ccagcttggt gatttgcagg ttatgtttgt ggtcaaggcc
    13261 aggaacttta ttcagtacct ggatatccac gccttcatca ggttgcagac ggatagtcag
    13321 tttattctgc ggcagatcct gccacgactc tttaaacaga ttcagttcag gtgttttgaa
    13381 atagaccacg acttcagaac atttggtcgg cagacgttta ccagtacgca ggtagaatgg
    13441 cacaccggcc cagcgccagt tatcaatgtc gacgcggatc gccacgaagg tttctgtatt
    13501 gctgctcttg ttcgcgccct cttcttccag atatcccggc acttttttgc cctgggcgaa
    13561 gcccgcagta tattgcccgc gtacggtttt ttcgcgtacg ttggagcggt cgatgcggcg
    13621 cagggacttc agtactttca ctttttcatc gcggatgctg tctgcgctca ggtcagatgg
    13681 cggagacatc gcaatcatgc agagaatttg tagcaggtgg ttctgaatca tgtcacgcat
    13741 ctgaccggct ttatcaaaat agccccagcg cccttcgatc cccacttctt ctgccacggt
    13801 aatctcaaca tgatcaatgg tgcgattgtc ccagttattc acaaacaggg agttagcaaa
    13861 acgcagcgcc aacaggttca gcaccgtttc tttaccaaga tagtggtcga tacggtaaac
    13921 ctggcactcc tcgaagtatt cgccaacctg atcattgatt tcctgcgagg tcgccagcga
    13981 cgtccccagc ggtttctcca tgactacgcg tgccggttta gcattcagtt ttgcctcgcc
    14041 aagccctttg caaattgcgc caaaagtgct gggcggcatg gcaaagtagt taatggtgat
    14101 acgatttttt tgatccagca tcgcgccgag acggttgaat gcagcagtgt cattgacatc
    14161 gagattacaa aaatccagac gtgcactcag ggtgtcccat aaaccttcat caatggtttc
    14221 tttcatgaaa gtttcgagcg cctcgcggac aactttggta tatgccgctt tatcccagtc
    14281 agcacgccct acgccgataa tccgggtgtc cgggttgagc tgaccggctt tttccagttg
    14341 atacagggaa ggcagcaatt tacgacgcgc aaggtcgcct ttcgcgccga aaatgaccag
    14401 gtcacaggcc tgggctgttt gcgttaccgc catgtcattc tccttaagtt aactaacccg
    14461 gtacttaagc cagggtatac ttgtaatttt cttacggtgc actgtactgc ttttacgagc
    14521 ttgcgaaaac tgtaaacgct tatccacccg tgcgattacg ggaaaagcgc gcaaagtgcg
    14581 gcaaaactga taaaaaaatc atcgttttcc attcggtaaa aatctgacac tgatcatgtt
    14641 atgaaaaaaa ataacaactt ttttatctgc ttttgtcatt aacacggcac acaggcgtaa
    14701 tatctgacaa aactgcattt cgatttcttt cagtgcggaa atcgtcatta cccgtgagtc
    14761 tctttacatc atgaatatgc tggaaaaaat ccagtctcag ctggaacatt tgagcaaatc
    14821 agagcgcaaa gttgccgagg tcattctggc ttcgcccgat aacgcgatcc attcgagtat
    14881 tgctgctatg gcactggaag ccaatgttag cgaaccgacg gtgaatcgtt tctgtcgcag
    14941 catggacacg cgcggttttc ctgattttaa acttcatctg gcacagagtc tggcgaatgg
    15001 cactccctat gttaatcgca atgtcaatga agatgacagc gttgaatcat acacagggaa
    15061 aatatttgag tccgcaatgg caacgcttga tcatgtccgt cattcactgg ataaatctgc
    15121 catcaaccgc accgtcgact tgctcactca ggcaaaaaaa atcgcctttt tcggattagg
    15181 ctcttcagcc gccgttgccc acgatgcgat gaataagttc tttcgtttta atgttccggt
    15241 ggtgtactcc gatgatatcg tgctgcaacg catgagttgt atgaattgta gcgacggaga
    15301 cgtggtggtg ctgatttctc acactggaag aacaaaaaat ctggtcgagc tggcgcagct
    15361 ggctcgcgaa aacgacgcaa tggtgattgc cctcacctct gcgggtaccc cgctcgcccg
    15421 ggaagcaacg ctggcaatta ccctcgacgt accggaagat actgacattt atatgcccat
    15481 ggtttctcga cttgcacagc tgaccgtgat agatgtgctg gcgacaggat ttactttgcg
    15541 acgcggtgca aaattcagag ataacttgaa gcgggtcaag gaagcgctga aggaatcgcg
    15601 ttttgataag cagttactta atttaagtga cgatcgctaa aaacgactgt cactgtccta
    15661 atcttatacg acatccgaat gagattaatt tatcgccatc gcggcgttat ttcattcgga
    15721 tttcatgttc aagcaacacc tggttgtttc agtcaacgga gtattacatg tccagaaggc
    15781 ttcgcagaac aaaaatcgtt accacgttag gcccagcaac agatcgcgat aataatcttg
    15841 aaaaagttat cgcggcgggt gccaacgttg tacgtatgaa cttttctcac ggctcgcctg
    15901 aagatcacaa aatgcgcgcg gataaagttc gtgagattgc cgcaaaactg gggcgtcatg
    15961 tggctattct gggtgacctc caggggccca aaatccgtgt atccaccttt aaagaaggca
    16021 aagttttcct caatattggg gataaattcc tgctcgacgc caacctgggt aaaggtgaag
    16081 gcgacaaaga aaaagtcggt atcgactaca aaggcctgcc tgctgacgtc gtgcctggtg
    16141 acatcctgct gctggacgat ggtcgcgtcc agttaaaagt actggaagtt cagggcatga
    16201 aagtgttcac cgaagtcacc gtcggtggtc ccctctccaa caataaaggt atcaacaaac
    16261 ttggcggcgg tttgtcggct gaagcgctga ccgaaaaaga caaagcagac attaagactg
    16321 cggcgttgat tggcgtagat tacctggctg tctccttccc acgctgtggc gaagatctga
    16381 actatgcccg tcgcctggca cgcgatgcag gatgtgatgc gaaaattgtt gccaaggttg
    16441 aacgtgcgga agccgtttgc agccaggatg caatggatga catcatcctc gcctctgacg
    16501 tggtaatggt tgcacgtggc gacctcggtg tggaaattgg cgacccggaa ctggtcggca
    16561 ttcagaaagc gttgatccgt cgtgcgcgtc agctaaaccg agcggtaatc acggcgaccc
    16621 agatgatgga gtcaatgatt actaacccga tgccgacgcg tgcagaagtc atggacgtag
    16681 caaacgccgt tctggatggt actgacgctg tgatgctgtc tgcagaaact gccgcagggc
    16741 agtatccgtc agaaactgtt gcagccatgg cgcgcgtttg cctgggtgcg gaaaaaatcc
    16801 cgagcatcaa cgtttctaaa caccgtctgg acgttcagtt cgacaatgtg gaagaagcta
    16861 ttgccatgtc agcaatgtac gcagctaacc acctgaaagg cgttacggcg attattacca
    16921 tgaccgaatc gggtcgtacc gcgctgatga cctcccgtat cagctctggt ctaccaattt
    16981 tcgccatgtc gcgccatgaa cgtacgctga acctgactgc tctctatcgt ggcgttacgc
    17041 cggtgcactt tgatagcgct aatgacggcg tagcagctgc cagcgaagcg gttaatctgc
    17101 tgcgcgataa aggttacttg atgtctggtg acctggtgat tgtcacccag ggcgacgtga
    17161 tgagtaccgt gggttctact aataccacgc gtattttaac ggtagagtaa tctcacctgc
    17221 cccggccgct gatggccggg gtatttagca acacataaat taggactact tattcaatcc
    17281 aatcataatc acagccccct gtaaaatgaa tacagggata gcctgaatgg agatctaaat
    17341 agcttgcaag acgtcgcata cccgcttcct ctgcaacact atgtccaata actgccatcg
    17401 gtaagccact atctttggag tattgtgtct gcacccatgt tttaattgaa tcgttaatcg
    17461 taatataata atcagcctga tattccatat attgtattgg atcacaaaaa cagcctgccc
    17521 caatgccaac agttctgact actctggctt tatctccata aaattgaatg ccaggaagat
    17581 tatatatttt caaatttttg gcaaaacgtt tggtaatgtt aatggcactg tcagggtcta
    17641 tagcatatac acagtacatc tcatcagaag caacaatatt tgtaacatcc agttctaact
    17701 gctgagccaa agccttagac attccgaacg taggctctcg atccatgaca tcatggcaac
    17761 gaatgattac caaattattt ttatgcaaaa actccttttt ttgctcaatt atttttaaat
    17821 aactgagttc accgcgagta taatccatta tttttctggc ccatgaaaac tcaagatcgt
    17881 caccctccag atcagcataa gaataaaatg taggctcatg tgtaataatg atattgacac
    17941 cttcaaaaac agcctttctt aaaacatcca ttgaggcaat ccagcaggtc ccaattttat
    18001 taataagtgt cgcctcagca ccaaaaataa ttttatcaac agttggttgc gaaacagcaa
    18061 ccattgaatt taaaaaacaa ttaagatccg cggctgtttt aatatgatgc attcaacatc
    18121 cttcacaggt ttcatcagca ataaaataat ttcaacattt ctgggaaacg ttttgcccat
    18181 gcagactcag agtgggtatc acctccccat aaat
//
LOCUS       DADMKF010000075        17959 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.076, whole genome shotgun sequence.
ACCESSION   DADMKF010000075 DADMKF010000000
VERSION     DADMKF010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17959
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.076"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(229..1203)
                     /gene="cysB"
                     /locus_tag="JNC24_002379"
     CDS             complement(229..1203)
                     /gene="cysB"
                     /locus_tag="JNC24_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HAY9159109.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGSPTQIADAVSKGNADFTIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
     gene            complement(1413..4010)
                     /gene="topA"
                     /locus_tag="JNC24_002380"
     CDS             complement(1413..4010)
                     /gene="topA"
                     /locus_tag="JNC24_002380"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HAY9159110.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTIGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVNKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKIGEIVTDRLEENFRELMNYDFTAQMENSLDLVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            4390..4641
                     /locus_tag="JNC24_002381"
     CDS             4390..4641
                     /locus_tag="JNC24_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HAY9159111.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FIGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            complement(4677..5726)
                     /gene="sohB"
                     /locus_tag="JNC24_002382"
     CDS             complement(4677..5726)
                     /gene="sohB"
                     /locus_tag="JNC24_002382"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HAY9159112.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            5946..6704
                     /locus_tag="JNC24_002383"
     CDS             5946..6704
                     /locus_tag="JNC24_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HAY9159113.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSENCQQLAQRIVVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVMQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            6701..7291
                     /gene="cobO"
                     /locus_tag="JNC24_002384"
     CDS             6701..7291
                     /gene="cobO"
                     /locus_tag="JNC24_002384"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HAY9159114.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPIKHAFDAGVKAQIGIDY"
     gene            complement(7331..8203)
                     /gene="rluB"
                     /locus_tag="JNC24_002385"
     CDS             complement(7331..8203)
                     /gene="rluB"
                     /locus_tag="JNC24_002385"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HAY9159115.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGGRRSGGRNNG"
     gene            complement(8416..9530)
                     /locus_tag="JNC24_002386"
                     /pseudo
     CDS             complement(8416..9530)
                     /locus_tag="JNC24_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309867.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
     gene            complement(9558..10178)
                     /locus_tag="JNC24_002387"
     CDS             complement(9558..10178)
                     /locus_tag="JNC24_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HAY9159116.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            complement(10175..11056)
                     /gene="yciV"
                     /locus_tag="JNC24_002388"
     CDS             complement(10175..11056)
                     /gene="yciV"
                     /locus_tag="JNC24_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exoribonuclease"
                     /protein_id="HAY9159117.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEVLVHRAVEMRVGTLAITD
                     HDNTAAIVPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQLIAERLEKAQIPGALEGARRLAQGGAVTRGHFARFLVGCGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLAALARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            11194..11238
                     /gene="trpL"
                     /locus_tag="JNC24_002389"
     CDS             11194..11238
                     /gene="trpL"
                     /locus_tag="JNC24_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HAY9159118.1"
                     /translation="MKAIFVLKAWWRTS"
     gene            11330..12892
                     /gene="trpE"
                     /locus_tag="JNC24_002390"
     CDS             11330..12892
                     /gene="trpE"
                     /locus_tag="JNC24_002390"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HAY9159119.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGNRPATLLLESADID
                     SKDDLKSLLLVDSALRITALGDTVTIQALSGNGEALLALLDNALPAGVENEQSPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKNTRIQASLFAPNEEEKQRLTARLNDLRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSSYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSIPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     gene            12892..14487
                     /gene="trpD"
                     /locus_tag="JNC24_002391"
     CDS             12892..14487
                     /gene="trpD"
                     /locus_tag="JNC24_002391"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HAY9159120.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPILMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAD
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGEIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            14491..15849
                     /gene="trpCF"
                     /locus_tag="JNC24_002392"
     CDS             14491..15849
                     /gene="trpCF"
                     /locus_tag="JNC24_002392"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HAY9159121.1"
                     /translation="MQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDALQ
                     GARTAFILECKKASPSKGVIRDDFDPARIASIYKHYASAISVLTDEKYFQGSFDFLPI
                     VSQISPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDDQYRQLAAVAHSLEMGV
                     LTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGIN
                     TYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAGA
                     IYGGLILVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQLH
                     GNEDQLYIDTLREALPAHVAIWKALSVGETLPARELQHVDKYVLDNGQGGSGQRFDWS
                     LLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTLR
                     AY"
     gene            15861..17054
                     /gene="trpB"
                     /locus_tag="JNC24_002393"
     CDS             15861..17054
                     /gene="trpB"
                     /locus_tag="JNC24_002393"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016158396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HAY9159122.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPEKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            17054..17860
                     /gene="trpA"
                     /locus_tag="JNC24_002394"
     CDS             17054..17860
                     /gene="trpA"
                     /locus_tag="JNC24_002394"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HAY9159123.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPIEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISTPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKVATRS"
BASE COUNT         4315 a         4750 c         4487 g         4407 t
ORIGIN      
        1 tccagcgaat ttacgaatcg tctccagtga ataattcaat cttttgacct gaaatagata
       61 tttgaaaact ggagtttgcg aggcgggtaa ttagacacta ctttacaggt aatttaccct
      121 ataaatatga gggataaaaa tattgtgcac ggtatagaaa taagtaacaa aagagaatat
      181 attccggcac cttcgctaca taaaaggtgc cgaaaataac gcaagaaatt atttttccgg
      241 cagttttata tctttaaaca tgacctcaat ttcttcatta gagcgcaatg cgacagccgc
      301 atcaacgaca tcacgcgtta aatgcggtgc aaaacgctga atgaaatcat acatataact
      361 gcgcaagaaa gtactacggc gaaaaccaat tttggttgta ctgtggctga agatatcgtg
      421 agcatcaaca cgcacaaggt cgggatcggc gaccggatcc accgccatgc tggcaatgac
      481 ccctaccccc agccctaacc ggacgtaagt tttaatgacg tcagcatccg ttgccgtgaa
      541 gacgatacgc ggcgttaacc ctgcgcggtt aaaggcagta tccagttctg aacgtccggt
      601 aaagccgaag gtatatgtca ccaacggata ttgcgccagt tcttcaatgg taatggcttt
      661 tttgcctgcc agcgggtgat ccggagtgac tacaatagcc cgattccagt ggtagcacgg
      721 taacatcact aaatcttcat acagatgcag cgcttctgtg gcgatagtga aatcagcatt
      781 gcctttagag acggcatcag caatttgtgt cggcgagccc tggtgcatat gcaaagaaac
      841 gcggggataa cgctcaataa agcctttgat aacgtttggt aacgcgtagc gtgcctgggt
      901 atgcgtggtg gcgatataca gtgagccttt atccggccag gtgtgctctc cggcaaccga
      961 ttttatggca tcgactttcg acaggacttc gcgagcgata cgaattattt cttgccctgc
     1021 tggcgttacc tgcgtcaggt gcttgccgct tcgggaaaaa atttgaatgc ctagctcgtc
     1081 ttccagcatt ctgacttgtt tactgatccc gggttgtgat gtgtaaagtc cttccgctgt
     1141 tgatgagaca ttcaggttat gattgaccac ctcaacaata tagcgaagtt gttgtaattt
     1201 catgttaaac catccactta gacgccatca gaacatcgtt ttttcgtcac acctggcggg
     1261 tgactaatac gatttaataa taaaaaaggt gctaactata accactatat catttatagc
     1321 cttagtgtat aggtgtgaag aaaaaataat atgcaataaa aaagggccgc tttcgcgacc
     1381 ctttgtttat aaaaacctga cagaattaaa ggttattttt ttccttcaac ccatttgcca
     1441 tcaacataaa atgctgacca gccagtcgcc tttccgtctt tttccgaaga gacatattgc
     1501 tgtttggttt tacggctaaa gcgaaccatg gtcttattac cttccggatc ctgctgtggc
     1561 gcatcggcca gataacgcag tttttccggc agacggtcgc ggaagcgata aagctcttcc
     1621 accagtggcg cacgcgtttc acgcgatttc gggaaagtgt tggcagccag gaaaacaccc
     1681 gcagcgccgt cacgcagcac gaaataagca tctgattttt cgcacggcag ctcaggtaat
     1741 ggcaccggat cttctttcgg tggtgccact tcgccgttac gtaaaatctt acgtgtgttt
     1801 ttacactctt cgttggtgca ggccatgtat ttaccgaaac gccccatttt caggtgcatt
     1861 tcagagccac atttttcaca ctcaacgatc gggccgtcat aacctttaat gcggaattcg
     1921 ccctcttcga tctcgtaacc gtcgcaggtt gggttattac cacagacatg caacttacgt
     1981 ttcggatcga tgagatagct gtccatcgcc gtgccgcatt tcgggcaacg acgttttgcg
     2041 cgcagcgcgt tggtttcagc gtcttcgcct tccagtacgt tcagcacttc gttttccggc
     2101 accaggttaa tggtggtttt gcaacgctct ttcggcggca gcgcataacc agaacagcca
     2161 aggaataccc cggtgctcgc tgtgcgaatc cccattttgc gaccacaagt cgggcagtca
     2221 atgctggtca gaaccatctg gttcgggcgc ataccaccct cttccggatc tttttcagct
     2281 ttatctaact gctgggtgaa atccgagaag aagtgatcca gtacagcttt ccactctgct
     2341 tcgtgatttg ccaccaggtc gaggctgttt tccatctgtg cggtgaagtc gtagttcatt
     2401 aactcgcgga aattttcttc aaggcgatcg gtgacgattt cgccaatttt ttccgcatag
     2461 aaacgacgat tttctactcg cacgtagcca cgatcctgaa tggtcgaaat gatcgacgca
     2521 taggtagacg gacgaccgat accgcgtttt tccagctctt taaccagcga tgcttcactg
     2581 aaacgggctg gcggcttggt aaagtgctgg gctggtgtaa gttcaacgag cgtcagagca
     2641 tcgcctttat taactgctgg taagatgcga tcttcatcgc ctttacgcaa cgcaggcatc
     2701 acttttgtcc agccatcaaa acgcaaaata cgaccgcgtg ctttcaggcg gaaatcgccc
     2761 gcaccaatgg tcagcgtcgt ggagtcatat ttcgctgggg tcatctggca ggctacgaac
     2821 tgacgccaga ttaactggta cagtttctgc gcatctgctt ccatatcctt cagcgattcc
     2881 gccatcacat tgacgtcaga aggacgaatc gcttcgtgcg cttcctgtga gttttctttg
     2941 ctggcgtact gattcggact ttccggcaga tatttcttac caaaattatc gctgatataa
     3001 ccacgaacca tatttaccgc gtcctgactc aggttagtgg agtcggtacg catgtaagtg
     3061 atatagcctg cttcatacaa acgctgcgcc atcatcatgg tttttttcac gccaaatcca
     3121 agacgggtgc tggcagcttg ttgcagcgta gaggtaataa aaggagcgcc aggtttactg
     3181 gttgtcggtt tgtcttcacg ttccagcacg ctgtagcgcg ctttttccag cagacttacc
     3241 gcagcctgag tttgttcttt gttgaccgga cggaacggtt tgtcgttctg atgagtcacc
     3301 tgtaacgcca acgcttcacc agatggcgtg gtcgtgctgg catcgacttc ccagaactct
     3361 tccggcacga acgctttaat ttcacgctca cgctcgacca ccaggcgaac cgccaccgac
     3421 tgcacacgac cggcagacag accacgagcg atctttttcc atagcagcgg cgaaaccata
     3481 taccccacca cgcggtccat aaagcgacgc gcctgctggg cattaacacg atcaatattc
     3541 agctcacccg gtttgttaaa tgcctggcgg atcgcgtttt tagtaatttc gttaaacacc
     3601 actcggctat agcgcgcatc atcaccccca atcacttccc gcaggtgcca tgcaatggct
     3661 tccccttcgc ggtcaaggtc ggttgcgaga tagatgtggt cggctttttc agccagttgt
     3721 ttcagttcag agacgacctt ctctttacca ggcaacactt catagtgcgc ctcccaattg
     3781 tgccacgggt caacccccat acggttgacg agagcgccac gttcatcctt tttaggcttt
     3841 ttagccgtct tggtggaggt agagtcggca ctctttttgg cagctgagcc actggtcggc
     3901 aaatcgcgga tgtgaccgac gctggatttc accacgtagt cactacccag atacttgttg
     3961 atcgttttgg cttttgccgg ggactcaacg atgacaagag ctttacccat attcacctta
     4021 cctaatttga ttcttccagg aatgcgtcgc acgttgataa accttccact ggcaacgagt
     4081 taccgatatg gactcgttct ctgcggatat caaccccttg tcgtgactct gttcacgaaa
     4141 attcaggtga ctgagttaac aggtcttggc ctacaggcgc tatagcacga cggaattttg
     4201 gtcgaatgtc aagcaaatct attgccagat tgacgaaagc gtcacactgt acctgataaa
     4261 atccgatacg caactttatt agcatgcaaa atccaaagtt gccagtaagg cgctcttgcc
     4321 accagcgtgg tatatttgtc tgcaagccat gcctggcgta gactaatgtc cttgtttaag
     4381 gagtaaataa tgaacaaaga gacacaaccc atcgaccgcg aaacgctgtt gaaagaggcc
     4441 aacaaaatca ttcgtgagca tgaggatacg ctcgcaggga ttgaagccac cggcgtcacg
     4501 cagcgtaatg gcgtgctggt atttatcggc gactactttt tagatgaaca aggattgcca
     4561 acagccaaaa gcaccgcggt gtttaatatg ttcaaacacc tggcccatgt gctttcagaa
     4621 aaatatcatc tggttgatta aagcaaaacg cgaggtctta gcctcgcgtt tgtcttttac
     4681 atcaatggct tttgaccccg ctgccaccag cgtagcaaca atcgatcggc gctctctgcc
     4741 gcgctgccgg tgaatcggtc aatgagtcgt ttacgctgca tatagcgtac attaaccact
     4801 tcacggcctt ccatcaggct aagaataact tcatcactgg tgttgatttc atcaaccagg
     4861 cctttctcta ccgcctgttg tccgtaccag tgttcacccg ttgccacctg ttcaatatcc
     4921 agagacggac gcatacgctt cacaaaatct ttaaataact gatgcgtttc gttcagctct
     4981 tcgcggaatt tctcccgccc ttcttcggta ttttcaccca gcaaggtcag cgtgcgctta
     5041 tactgcccgg cggtgtgcag ttcgatatca atatctttgc ttttcaggaa gcggttaaag
     5101 ttgggcattt gcgccaccac cccaatggaa cccacaatag caaacggtgc ggaaacaatt
     5161 ttgtccgcca cacaggccat catgtaaccg ccgctggcag cgactttgtc taccgtaaca
     5221 gttaaaggaa tgtttttatc acgcagacgc tgcagctgcg aagccgccaa cccgtaacca
     5281 tgcaccatgc caccagggct ttccagacgg agcacaacct gatcctgcgg tttgaatgct
     5341 gcgagtaccg ccgttatctc ttcacgtagc gagttcactt catgggcgtc catgctgcct
     5401 ttaaaatcca gcacccagac gcgaggttta ctgtcagttg ccacctcgcc cagtttggct
     5461 ttcgcttttg ctgctttcgc ttcttgcttg tgctttttct tctgcgcttt gtgccactgt
     5521 ttttgctgat gtgagtccat cagcgccgcg gccagttctt ctttcatctc cttatactgt
     5581 tcgctgagat tgttgacccg taactcgcca cgctggcgtt tattacgttg agcaacattg
     5641 acaataatgg cggcaatcgc cgcaatcgct agcacaacgg taacgatttt cgccaaaaac
     5701 aaaccatatt cagacaacaa ttccacgagt ccccaccttg gttaaacaac gctttggctc
     5761 acagtttaca acaggcatgt taagccgtct cgcagagatg ttattgcagc gaaagtatga
     5821 caaagtatcc attttgcggt gttatcgccc caggctgatt gaaatgccct tctgtttcag
     5881 gcataaagcc ccaaagtcat aaagtacact ggcagcgcga cagacaacgt cgttgaggag
     5941 tagccatgca ttaccagcca aaacaagatt tactcaatga tcgcattatc ctggtgacgg
     6001 gagccagcga tgggattggt cgtgaagccg cgatgacgta tgcacgctat ggtgcgacag
     6061 tgattctgtt gggccgtaat gaagaaaaat tacgtcaggt agccagccac ataaacgaag
     6121 aaactgggcg tcagccacag tggtttattc tcgatttgct gacctgcacg tccgaaaatt
     6181 gccaacaact ggcacagcgc attgtcgtta attatccgcg tctggatggt gttttgcata
     6241 atgccggatt gctcggcgat gtttgcccaa tgagcgaaca aaatccgcag gtctggcagg
     6301 acgtcatgca ggtcaacgtt aatgccacct ttatgctcac ccaggcactg cttcctttat
     6361 tactcaaatc ggacgccggt tcactggtct ttacttcatc aagcgttggg cgtcagggac
     6421 gagccaactg gggtgcatat gcagcgtcga aatttgccac cgaagggatg atgcaggtac
     6481 tggccgatga atatcagcag cgcctgcgcg tcaactgcat taacccaggc ggtacgcgca
     6541 ccgcaatgcg cgcaagcgcc ttcccgaccg aagatccaca gaaacttaaa acacccgctg
     6601 atatcatgcc gctctacctc tggctgatgg gcgatgacag ccgccgtaaa accggcatga
     6661 cctttgacgc ccaaccgggc cgtaaaccag gaatttccca atgagtgatg aacgctacca
     6721 acagcgtcag cagcgagtga aagaaaaagt agatgctcgt gtggcccagg cccaggatga
     6781 acgcggtatt atcatcgtct ttaccggcaa tggaaaaggc aaaaccaccg cggcatttgg
     6841 tacggcaaca cgcgcagttg gtcacggaaa aaaagtgggc gtcgtgcagt ttattaaagg
     6901 cacctggcct aatggcgaac gcaatctgct ggagccacat ggcgttgagt ttcaggtgat
     6961 ggcaacgggc tttacctggg atacacaaaa ccgcgagtct gataccgccg cctgccgcga
     7021 agtctggcaa catgcaaagc ggatgcttgc tgattcctca ctggatatgg ttttgcttga
     7081 tgaactgacg tatatggtgg cgtatgacta tttgccactg gaagaagtgg tgcaggcgtt
     7141 aaatgaacgt ccacatcaac agacggtgat tatcacgggt cgaggctgtc atcgggatat
     7201 tcttgaactg gcggatacgg taagtgagct gcgccccatc aaacatgcgt ttgatgccgg
     7261 tgttaaggcg caaataggga ttgattatta atctgtaaaa atgccggata tgacgtccgg
     7321 catactttga ttaaccgtta ttacgcccgc cagaacgacg accgccgctc acctgactgt
     7381 gacgtttcac cgcacgacga atctgattag ccttcatgcg acgacggtct ttttctacag
     7441 cgactttaga gctggtttct ggcggaagct ccaccagttc gcgcagatag ttagtctggg
     7501 cgaggtccag ctccgtccag ccaccgcgtg gcagaccttt tggcagtggg atatcaccgt
     7561 aacgaacacg gatcaggcgg ctcacctgca caccaaccgc ttcccacaga cgacgaactt
     7621 cacggttacg gccttcggtc agagtcacgt tgtaccactg gttgatccct tcgccgccgc
     7681 tgaacttgat ggttttaaaa gctgccggac catcttccag ttgcacgcca cgactcaaat
     7741 cacgcagttt cgcgtcgtca acctgaccaa atacacgcac ggcgtattca cgttcaactt
     7801 cacggcttgg atgcattaaa cggtttgcca gttcaccatc ggtggtgaac agcagcagac
     7861 cacaggtgtt aacgtccaga cgacctacgg caatccagcg tgcgccacgc agctttggca
     7921 gacggtcaaa caccgtcgga cgcccttccg gatcgttacg ggtacacaac tcaccttccg
     7981 gtttgtaata ggccagaacg cgacaaattt gttcagcgga ctcacgtacc gaaatcagat
     8041 gaccatcgat acgaattttc agcccagggg taacttcaac gcgatcgccg agtttagcaa
     8101 ttttgccatc aacactcaca cgaccggctt caataataga ttcgatttca cggcgggagc
     8161 cgtggccagc gcgggccagc actttctgta atttttcgct catagagctt ccttttggtg
     8221 tcgccttccc aggcgtctga tatgcttttg tgcaacagtg agtcagaact tacatctgac
     8281 tcactgatag taaagtctgt ttgaactctt caggccttaa gtaaacgact cgacgtttta
     8341 actggcctgg ctttgaggcg caacatacta cactatcttt atggttaaag cttgtttcga
     8401 ttaacatcag cttaattacc agccaccacc gcctccgcca cccgcgccgc caccagcacc
     8461 accaccggaa taacttcgcc ccaacaaact ggttttggcc cccagactca ggtgcatctg
     8521 taatgaaaac aaaacgccac tacgggcaat ttccggggcg ctaacagccg ctgtgagtga
     8581 tggggtgata cgtgctaccc attctcttcc aaggtttgct gagactgccc aacctagcaa
     8641 cgcgtaatcc agtttccgtg tttccccttt ccgccgacgc ccactgtgtg ttgctgcctc
     8701 tttacgcgca agatagttaa cgatagcttc accgtgggca taacgttgct gcccagtttg
     8761 tgtaaacttt ggcagaaaaa tataccccat gccagtgaga taaccggtca ggaaaacagc
     8821 gctaaaataa cccgctggca tataccagta aaagaataca tctccgacat tgatgtagag
     8881 agaatagaag gccacgcctg aacaaatcag tggtaaacaa ttcttccgtc ctcaaggatg
     8941 cggccatttc gatatgtgtc gccctcttct ccagtaaaga gatcgatttc gctaaatgga
     9001 ttacccgcga tatcagggaa cttgagtgaa aatcgggtct tatagatttc aaacggccag
     9061 tggttaccag tcacgttcca gattaacaga tctgaatctc cctcacgcag gaaagcattt
     9121 ttaacctgat aatgaatttg ataatgatag tcgccttgag gcaagaaacg ttgtttatcg
     9181 cccacgacaa tactcattaa cccgatatgc cagtcatgat gccagggttc aggaatacca
     9241 tcacgggtga cgccaacaat atgatagttc agcagtgcag cgtccccacc aggctgcatc
     9301 cagagtcgtg gtaaatcacg ataaaatccg tgtttaatct gctgatttcg cgcctgaaaa
     9361 tgaatatttt ctttgatgtc gagcacccca tccggttgca gagtggcttc aacatcaaag
     9421 agcgaaatcc cttcttcata agaagggatc tccgtgtttt ctgcataact tagagatgat
     9481 acaaatagcc ctgctatcaa cagcaaaatg cagcgaaact tccctgccat agtcttccat
     9541 ccgtggaacg caatttgtta taagaaaggc ttcacatcac ctacgccttc acgcaccacg
     9601 accggcgtat catcggtaag atcaataacc gttgtcggtt tctgcccgag atagccgccg
     9661 tgaataatca aatctacctg tttttccaga cggtctttaa tttcttcggg atcggattcg
     9721 gtaaattcgc tgcctggcag cattagcgaa gtggaaagca tcggttcgcc cagtgcttca
     9781 agcaacgcct gcgcaatcgg gttagaaggc acacgcatac cgatggtttt gcgtttttcc
     9841 tgcaacagac ggcgtggcac ttccttcgtc cccttcagga tgaaggtata gttgcccggc
     9901 gtgttgtttt tcattaaacg aaacgcaacg ttatcaacaa atgaataggt cgagagttca
     9961 gaaagatcgc gacacatcag ggtaaagttg tgaccgtccg gcagctggcg aatacgacaa
    10021 atacgctcca tcgcgttttt atcttcaatt ttacagccga gcgcatagcc ggaatcagtt
    10081 ggataaacaa tcaccccgcc tttacgcacg atctccaccg cctggttgat cagacgttgc
    10141 tgtgggttat caggatgaat ataaaaaaac tggctcataa ttccctctct gtggtgttct
    10201 gcggctgttc ccatagctgc cagacgcctt caacgcctgc gggcagccag agtttacgac
    10261 ctagttcaat ccacgggcat ggctgatgaa aatcagatcc ttgtgatgcc cataaatgat
    10321 gctgacgcgc aagggccgcc agctgggtac gttcattggg cgattgctgg cactgcgcga
    10381 cttccatcgc gtcaccgtgg tgttcggcaa aatgcgcaac caatcttttc agccatttag
    10441 cactaagatt gtaccgccca ggatgagcta gcaccgcctt accgccagaa tgatgaatga
    10501 catcaatagc ttgttctatt gtacaccact gtggcggaac gtatccggtt ttcccgcgcg
    10561 ccagatattt tttaaagaca tccgccattg aactggcttt gccgcacccc acaaggaaac
    10621 gcgcaaaatg accgcgcgtc accgcaccgc cctgcgccag tcgtcgcgcg ccttccagtg
    10681 cccctggtat ttgcgctttt tcaagtcttt cggcaatcag ttgcgcccgc tgattgcgcc
    10741 gttctgtctg ctgcgcgagg aactcacaca ttagcggatg agtaatatca atattcagcc
    10801 cgacaatatg aatttcatga ttttcccaga ccgtggaaat ttccacgccg ggaataagat
    10861 tcagcgccag tcccgaacgt gaaatttcct ctcttgctgg cacgattgca gctgtgttgt
    10921 catggtcggt gatcgccagg gtaccgacgc gcatctcgac tgcacggtgc accaatactt
    10981 ctggcgtcag gcagccatcg gaagctgtgg tatggctgtg caggtcgtaa atcactgcgt
    11041 aattcgtgtc gctcaaggcg cactcccgtt ctggataatg ttttttgcgc cgacatcata
    11101 acagttctgg caaatattct gaaatcagct gttgacaatt aatcatcgaa ctagttaact
    11161 agtgcgcaag ttcacgtaaa aagggtatcg acaatgaaag caattttcgt actgaaagct
    11221 tggtggcgca cttcctgaaa cgggcagtgt attcaccatg cgtaaagcaa tcagataccc
    11281 agcccgccta atgagcgggc ttttttttga acaaaattag agaataacaa tgcaaacaca
    11341 aaaaccgact ctcgaactgc taacctgcga aggcgcttat cgcgacaacc cgactgcgct
    11401 ttttcaccag ttgtgtggga atcgtccggc aacgctgctg ctggaatccg cagatatcga
    11461 cagcaaagat gatttaaaaa gcctgctact ggtagacagt gcgctgcgca ttacagcatt
    11521 aggtgacact gtcacaattc aggcactttc cggcaacggc gaagccctgc tggcactact
    11581 ggataacgcc ctgcctgcgg gtgtggaaaa tgaacaatca ccaaactgcc gtgtgctgcg
    11641 cttcccccct gtcagtccac tgctggatga agacgcccgc ttatgctccc tttcggtttt
    11701 tgacgctttc cgtttattgc agaatctgtt gaatgtaccg aaggaagaac gagaagcaat
    11761 gttcttcggc ggcctgttct cttatgacct tgtggcggga tttgaagatt taccgcaact
    11821 gtcagcggaa aataactgcc ctgatttctg tttttatctc gctgaaacgc tgatggtgat
    11881 tgaccatcag aaaaaaaaca cccgcattca ggccagcctg tttgctccga atgaagaaga
    11941 aaaacaacgt ctcactgctc gcctgaacga tcttcgccag cagctgaccg aagccgcgcc
    12001 gccgctgccg gtggtttccg tgccgcatat gcgttgtgaa tgtaatcaga gcgatgaaga
    12061 gttcggtggc gtagtgcgtt tgttgcaaaa agcgattcgc gctggagaaa ttttccaggt
    12121 ggtgccatct cgccgtttct ctctgccctg cccgtcaccg ctggcggcct attacgtgct
    12181 gaaaaagagt aatcccagcc cgtacatgtt ttttatgcag gataatgatt tcaccctgtt
    12241 tggcgcgtcg ccggaaagtt cgctcaagta tgacgccacc agccgccaga ttgagatcta
    12301 cccgattgcc ggaacacgtc cacgcggtcg tcgtgccgat ggctcgctgg acagagacct
    12361 cgacagccgc atcgaactgg aaatgcgtac cgatcataaa gagctttctg aacatctgat
    12421 gctggtggat ctcgcccgta atgatctggc acgcatttgc acccccggca gcagctacgt
    12481 cgccgatctt accaaagttg accgttactc ttacgtgatg cacctggtct cccgcgtggt
    12541 cggtgagctg cgccacgatc tcgacgccct gcacgcttac cgcgcctgta tgaatatggg
    12601 gacgttaagc ggtgcgccga aagtacgcgc catgcagtta attgccgagg cggaaggtcg
    12661 tcgccgcggc agctacggcg gcgcggtagg ttatttcacc gcgcatggcg atctcgacac
    12721 ctgcattgtg atccgctcgg cgctggtgga aaacggtatc gccaccgtgc aagccggtgc
    12781 tggcgtagtc cttgattcta ttccgcagtc ggaagccgac gaaacccgta ataaagcccg
    12841 cgctgtactg cgcgctattg ccaccgcgca tcatgcacag gagactttct gatggctgac
    12901 attctgctgc tcgataatat cgactctttt acgtacaacc tggcagatca gttgcgcagc
    12961 aatgggcata atgtggtgat ttaccgcaac catattccgg cgcagacctt aattgaacgc
    13021 ctggcgacga tgagcaatcc gatactgatg ctttctcctg gccccggtgt gccgagcgaa
    13081 gccggttgta tgccggaact cctcacccgc ttgcgtggca agctgcccat tattggcatt
    13141 tgcctcggac atcaggcaat tgtcgaagct tacgggggct atgtcggtca ggcgggcgaa
    13201 attcttcacg gtaaagcgtc gagcattgaa catgacggtc aggcgatgtt tgccggatta
    13261 acaaacccgc tgccggtggc gcgttatcac tcgctggttg gcagtaacat tccggccggt
    13321 ttaaccatca acgcccattt taatggcatg gtgatggcag tacgtcacga tgcggatcgc
    13381 gtttgtggat tccagttcca tccggaatcc attctcacca cccagggcgc tcgcctgctg
    13441 gaacaaacgc tggcctgggc gcagcagaaa ctagagccag ccaacacgct gcaaccgatt
    13501 ctggaaaaac tgtatcaggc gcagacgctt agccaacaag aaagccacca gctgttttca
    13561 gcggtggtgc gtggcgagct gaagccggaa caactggcgg cggcgctggt gagcatgaaa
    13621 attcgcggtg agcacccgaa cgagatcgcc ggggcagcaa ccgcgctact ggaaaacgca
    13681 gcgccgttcc cgcgcccaga ttatctgttt gctgatatcg tcggtactgg cggtgacggc
    13741 agcaacagta tcaatatttc taccgccagt gcgtttgtcg ccgctgcctg tgggctgaaa
    13801 gtggcgaaac acggcaaccg tagcgtctcc agtaaatctg gttcgtccga tctgctggcg
    13861 gcgttcggta ttaatcttga tatgaacgcc gataaatcgc gccaggcgct ggatgagtta
    13921 ggtgtttgtt tcctctttgc gccgaagtat cacaccggat tccgccacgc gatgccggtt
    13981 cgccagcaac tgaaaacccg caccctgttc aatgtgctgg ggccattgat taacccggcg
    14041 catccgccgc tggcgttaat tggtgtttat agtccggaac tggtgctgcc gattgccgaa
    14101 accttgcgcg tgctggggta tcaacgcgcg gcggtggtgc acagcggcgg gatggatgaa
    14161 gtttcattac acgcgccgac aatcgttgcc gagctgcatg acggcgaaat taagagctat
    14221 caattgaccg ctgaagattt tggcctgact ccctaccacc aggagcaact ggcaggcgga
    14281 acaccggaag aaaaccgtga cattttaaca cgcttgttac aaggtaaagg cgacgccgcc
    14341 catgaagcag ccgtcgctgc gaacgtcgcc atgttaatgc gcctgcatgg ccatgaagat
    14401 ctgcaagcca atgcgcaaac cgttcttgag gtactgcgca gtggttccgc ttacgacaga
    14461 gttaccgcgc tggcggcacg agggtaaatg atgcaaaccg ttttagcgaa aatcgtcgca
    14521 gacaaggcga tttgggtaga aacccgcaaa cagcagcaac cgctggccag ttttcagaat
    14581 gaggttcagc cgagcacgcg acatttttat gatgcgctac agggtgcacg cacggcgttt
    14641 attctggagt gcaagaaagc gtcgccgtca aaaggcgtga tccgtgatga tttcgatccg
    14701 gcacgcattg cctccattta taaacattac gcttcggcaa tttcggtgct gactgatgag
    14761 aaatattttc aggggagctt tgatttcctc cccatcgtca gccaaatctc cccgcagccg
    14821 attttatgta aagacttcat tatcgaccct taccagatct atctggcgcg ctattaccag
    14881 gccgatgcct gcttattaat gctttcagta ctggatgacg accaatatcg ccagcttgca
    14941 gccgtcgctc acagtctgga gatgggggtg ctgaccgaag tcagtaatga agaggaactg
    15001 gagcgcgcca ttgcattggg ggcaaaggtc gttggcatca acaaccgcga tctgcgtgat
    15061 ttgtcgattg atctcaaccg tacccgcgag cttgcgccga aactggggca caacgtgacg
    15121 gtaatcagcg aatccggcat caatacttac gctcaggtgc gcgagttaag ccacttcgct
    15181 aacggttttc tgattggttc ggcgttgatg gcccatgacg atttgcacgc cgccgtgcgc
    15241 cgggtgttgc tgggtgagaa taaagtatgt ggtctgacgc gtgggcaaga tgctaaagca
    15301 gcttatgacg cgggcgcgat ttacggtggg ttgattttgg ttgcgacatc accgcgttgc
    15361 gtcaacgttg aacaggcgca ggaagtgatg gctgcggcac cgttgcagta tgttggcgtg
    15421 ttccgcaatc acgatattgc cgatgtggtg gacaaagcta aggtgttatc gctggcggca
    15481 gtgcaactgc atggtaatga agatcagctg tatatcgata cgctgcgtga agctctgcca
    15541 gcacacgttg ccatctggaa agcattaagc gtcggtgaaa ccctgcccgc ccgcgagctt
    15601 cagcacgttg ataaatatgt tttagacaac ggccagggtg gaagcgggca acgtttcgac
    15661 tggtcactat taaatggtca atcgcttggc aacgttctgc tggcgggggg cttaggcgca
    15721 gataactgcg tggaagcggc acaaaccggc tgcgccggac ttgattttaa ttctgctgta
    15781 gagtcgcaac cgggcatcaa agacgcacgt cttttggcct cggttttcca gacgctgcgc
    15841 gcatattaag gaaaggaaca atgacaacat tacttaaccc ctattttggt gagtttggcg
    15901 gcatgtacgt gccacaaatc ctgatgcctg ctctgcgcca gctggaagaa gcttttgtca
    15961 gcgcgcaaaa agatcctgaa tttcaggccc agttcaacga cctactgaaa aactatgccg
    16021 ggcgtccaac cgcactgacc aaatgccaga acattacggc tggcacgaac accacgctgt
    16081 atctgaagcg cgaagatttg ctgcacggcg gcgcgcataa aactaaccag gtgctcggtc
    16141 aggctttact ggcgaagcgg atgggtaaaa ccgaaatcat cgccgaaacc ggtgccggtc
    16201 agcatggcgt ggcgtcggcc cttgccagcg ccctgctcgg cctgaaatgc cgtatttata
    16261 tgggtgccaa agacgttgaa cgccagtcgc ctaacgtttt tcgtatgcgc ttaatgggtg
    16321 cggaagtgat cccggtgcat agcggttccg cgacgctgaa agatgcctgt aacgaggcgc
    16381 tgcgcgactg gtccggtagt tacgaaaccg cgcactatat gctgggcacc gcagctggcc
    16441 cgcatcctta tccgaccatt gtgcgtgagt ttcagcggat gattggcgaa gaaaccaaag
    16501 cgcagattct ggaaagagaa ggtcgcctgc cggatgccgt tatcgcctgt gttggcggcg
    16561 gttcgaatgc catcggcatg tttgctgatt tcatcaatga aaccaacgtc ggcctgattg
    16621 gtgtggagcc aggtggtcac ggtatcgaaa ctggcgagca cggcgcaccg ctaaaacatg
    16681 gtcgcgtggg tatctatttc ggtatgaaag cgccgatgat gcaaaccgaa gacgggcaga
    16741 ttgaagaatc ttactccatc tccgccggat tggatttccc gtctgtcggc ccacaacacg
    16801 cgtatcttaa cagcactgga cgcgctgatt acgtgtctat taccgatgat gaagcccttg
    16861 aagccttcaa gacgctgtgc ctacacgaag ggatcatccc ggcgctggaa tcctcccacg
    16921 ccctggccca tgcgctgaaa atgatgcgcg aaaacccgga aaaagagcag ctactggtgg
    16981 ttaacctttc cggtcgcggc gataaagaca tcttcaccgt tcacgatatt ttgaaagcac
    17041 gaggggaaat ctgatggaac gctacgaatc tctgtttgcc cagttgaagg agcgcaaaga
    17101 aggcgcattc gttcctttcg tcacgctcgg ggatccgggc attgagcagt cattgaaaat
    17161 tatcgatacg ctaattgaag ccggtgctga cgcgctggag ttaggcatcc ccttctccga
    17221 cccactggca gatggcccga cgattcaaaa cgccactctg cgcgcctttg cggcaggtgt
    17281 gactccggca caatgttttg aaatgctggc actgattcgc cagaaacacc cgaccattcc
    17341 cattggcctg ttgatgtatg ccaatctggt gtttaacaaa ggcattgatg agttttatgc
    17401 ccagtgcgag aaagtcggcg tcgattcggt gctggttgcc gatgtgccaa ttgaagagtc
    17461 cgcgcccttc cgccaggccg cgttgcgcca taatgtcgca cctatcttca tctgcccgcc
    17521 aaatgccgat gacgacctgc tgcgccagat agcctcttac ggtcgtggtt acacctattt
    17581 gctgtcacga gcgggcgtga ccggcgcaga aaaccgcgcc gcgttacccc tcaatcatct
    17641 ggttgcgaag ctgaaagagt acaacgctgc acctccattg caggggtttg gtatttccac
    17701 cccggatcag gtaaaagcag cgattgatgc aggagctgcg ggcgcgattt ctggttcagc
    17761 cattgttaaa atcatcgagc aacatattaa tgagccagag aaaatgctgg cggcactgaa
    17821 agtttttgta caaccgatga aagtggcgac gcgcagttaa tccccctgcc gccagttccg
    17881 ctggcggcat tttaactttc tttaacgaag ccggaaaaat cctaaattca tttaatattt
    17941 atctttttac cgtttcgct
//
LOCUS       DADMKF010000076        17673 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.077, whole genome shotgun sequence.
ACCESSION   DADMKF010000076 DADMKF010000000
VERSION     DADMKF010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17673
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.077"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            309..860
                     /locus_tag="JNC24_002395"
     CDS             309..860
                     /locus_tag="JNC24_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HAY9159124.1"
                     /translation="MDLLEKQQAQLKSQLETIQKQQTGIISSTKTLTHVIKSVKDQQN
                     TFIFTEFNPAKTKYFILNNGSVALAGRVLSIDATENGSVIHISLVNLLSTPISNIGFN
                     ATWGGEKPVDAKEFARWQQLLFNTSMKSTLKLLPGQWQDINLTLKGVSPNNLGYLKLA
                     INMENIQFDNLPSAENRQKRSKK"
     gene            904..5520
                     /locus_tag="JNC24_002396"
     CDS             904..5520
                     /locus_tag="JNC24_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HAY9159125.1"
                     /translation="MNKIFKVIWNPATGNYTVTSETAKSRGKKSGRSKLLISALVAGG
                     MLSSFGALANAGNDNGQGVDYGSGSAGDSWVAIGKGAKANTFMNTSGSSTAVGYDAIA
                     EGQYSSAIGSKTHAIGGASMAFGVSAISEGDRSIALGASSYSLGQYSMALGRYSKALG
                     KLSIAMGDSSKAEGANAIALGNATKATEIMSIALGDTANASKAYSMALGASSVASEEN
                     AIALGRSSVASGTDSLAFGRQSLASAANAIAIGAETEAAENATAIGNNAKAKGTNSMA
                     MGFGSLADKVNTIALGNGSQALADNAIAIGQGNKADGVDAIALGNGSQSRGLNTIALG
                     TASNATGDKSLALGSNSSANGINSVALGADSIADLDNTVSVGNSSLKRKIVNVKNGAI
                     KSDSYDAINGSQLYAISDSVAKRLGGGAAVDVDDGTVTAPTYNLKNGSKNNVGAALAV
                     LDENTLQWDQTKGKYSAAHGTSSPTASVITDVADGTISASSKDAVNGSQLKATNDDVE
                     ANTANIATNTSNIATNTASIATNTTNITNLTDSVGDLQADALLWNETKKAFSAAHGQD
                     TTSKITNVKDADLTADSTDAVNGSQLKTTNDAVATNTTNIANNTSNIATNTTNISNLT
                     ETVTNLGEDALKWDKDNGVFTAAHGNNTASKITNILDGTVTATSSDAINGSQLYDLSS
                     NIATYFGGNASVNTDGVFTGPTYKIGETNYYNVGDALAAINSSFSTSLGDALLWDATA
                     GKFSAKHGTNGDASVITDVADGEISDSSSDAVNGSQLHGVSSYVVDALGGGAEVNADG
                     TITAPTYTIANADYDNVGDALNAIDTTLDDALLWDADAGENGAFSAAHGKDKTASVIT
                     NVANGAISAASSDAINGSQLYTTNKYIADALGGDAEVNADGTITAPTYTIANAEYNNV
                     GDALDALDDNALLWDETANGGAGAYNASHDGKASIITNVANGSISEDSTDAVNGSQLN
                     ATNMMIEQNTQIINQLAGNTDATYIEENGAGINYVRTNDNDLAFNDASASGVGATAVG
                     YNAVASGASSVAIGQNSSSTVDTGIALGSSSVSSRVIAKGSRDTSVTENGVVIGYDTT
                     DGELLGALSIGDDGKYRQIINVADGSEAHDAVTVRQLQNAIGAVATTPTKYFHANSTA
                     EDSLAVGEDSLAMGAKTVVNGNAGIGIGLNTLVLADAINGIAIGSNARANHANSIAMG
                     NGSQTTRGAQTGYTAYNMDAPQNSVGEFSVGSEDGQRQITNVAAGSADTDAVNVGQLK
                     VTDERVAQNTQSITNLNNQVTNLDTRVTNIENGIGDIVTTGSTKYFKTNTDGVDANAQ
                     GKDSVAIGSGSIAAADNSVALGTGSVAEEENTISVGSSTNQRRITNVAAGVNATDAVN
                     VSQLKSSEAGGVRYDTKADGSIDYSNITLGGGNGSTTRISNVSAGVNNNDAVNYAQLK
                     QSVQETKQYTDQRMVEMDNKLSKTESKLSGGIASAMAMTGLPQAYTPGASMASIGGGT
                     YNGESAVALGVSMVSANGRWVYKLQGSTNSQGEYSAALGAGIQW"
     gene            5888..7543
                     /gene="lldP"
                     /locus_tag="JNC24_002397"
     CDS             5888..7543
                     /gene="lldP"
                     /locus_tag="JNC24_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HAY9159126.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASKIDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLDKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            7543..8319
                     /gene="lldR"
                     /locus_tag="JNC24_002398"
     CDS             7543..8319
                     /gene="lldR"
                     /locus_tag="JNC24_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HAY9159127.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            8316..9506
                     /gene="lldD"
                     /locus_tag="JNC24_002399"
     CDS             8316..9506
                     /gene="lldD"
                     /locus_tag="JNC24_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HAY9159128.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPTALAPMAK
                     GNAA"
     gene            9702..10175
                     /gene="trmL"
                     /locus_tag="JNC24_002400"
     CDS             9702..10175
                     /gene="trmL"
                     /locus_tag="JNC24_002400"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HAY9159129.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(10228..11049)
                     /gene="cysE"
                     /locus_tag="JNC24_002401"
     CDS             complement(10228..11049)
                     /gene="cysE"
                     /locus_tag="JNC24_002401"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HAY9159130.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(11129..12148)
                     /gene="gpsA"
                     /locus_tag="JNC24_002402"
     CDS             complement(11129..12148)
                     /gene="gpsA"
                     /locus_tag="JNC24_002402"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAY9159131.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(12148..12615)
                     /gene="secB"
                     /locus_tag="JNC24_002403"
     CDS             complement(12148..12615)
                     /gene="secB"
                     /locus_tag="JNC24_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HAY9159132.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSVVSRGTFPQLNLAPINFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(12678..12929)
                     /gene="grxC"
                     /locus_tag="JNC24_002404"
     CDS             complement(12678..12929)
                     /gene="grxC"
                     /locus_tag="JNC24_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HAY9159133.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(13071..13502)
                     /locus_tag="JNC24_002405"
     CDS             complement(13071..13502)
                     /locus_tag="JNC24_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAY9159134.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKPVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            13747..15291
                     /gene="gpmM"
                     /locus_tag="JNC24_002406"
                     /gene_synonym="pgmI"
     CDS             13747..15291
                     /gene="gpmM"
                     /locus_tag="JNC24_002406"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HAY9159135.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLTGA
                     VDKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVAGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            15301..16584
                     /gene="envC"
                     /locus_tag="JNC24_002407"
     CDS             15301..16584
                     /gene="envC"
                     /locus_tag="JNC24_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HAY9159136.1"
                     /translation="MRGKAINTMTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADE
                     RDQLKSIQADIATKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNK
                     QIDEMNASIAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYF
                     GYLNQARQETIAQLKQTREEVAMQRAELEEKQSEQQTLLYEQRAQQAKLTQALNERKK
                     TLAGLESSIQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRDRQKEA
                     TRKGTTYKPTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIG
                     ASEGTEVKAIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQ
                     PIALVGSSGGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            16588..17547
                     /locus_tag="JNC24_002408"
     CDS             16588..17547
                     /locus_tag="JNC24_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HAY9159137.1"
                     /translation="MFPFRRNVLAFAALLALSSPVIAGKLAIVIDDFGYRPNNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNTVPDTPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKLNASVN"
BASE COUNT         4424 a         4568 c         4622 g         4059 t
ORIGIN      
        1 tatattcata caaacacaga catcttttga aacgaatgat acatttttat gatgaatgct
       61 taaaacaata tttaatcgac acacatcact gtcattaatg taaagcaaca tagatcacca
      121 ataatacaat ggacaactct accggcattg atcgctggtc ggaggaaata aggattttta
      181 catgaacttg aagaagattt ttttcagtgc tgtcactgtt tcagttctct gcgcattaac
      241 gggatgtgac tacattgaag aaggcaagcc agaaagtagt ttattgaaac aacaagaaga
      301 acattaaaat tgatctactt gaaaaacaac aagcacagct aaagagtcaa ctggaaacta
      361 ttcaaaaaca acaaactggc ataataagca gtacaaagac tttaactcac gtaattaaat
      421 ccgttaaaga tcaacaaaac acttttattt ttaccgagtt taacccagca aaaacgaagt
      481 atttcatcct gaacaatggt tcggtggctt tagcgggtcg agtgttatcc attgacgcca
      541 cagagaatgg cagtgttatt catatttcac tggtcaactt attaagtaca cctatctcaa
      601 atattggttt taatgcgaca tggggtggtg aaaaacctgt cgatgccaaa gagtttgccc
      661 ggtggcaaca attacttttc aacacatcaa tgaaatccac attgaaatta ttaccaggtc
      721 aatggcaaga cattaatttg accctgaagg gtgtatcgcc caataacctg ggatatctga
      781 agttagccat caacatggaa aatattcagt tcgacaatct tccctctgct gaaaatcggc
      841 agaaaagaag caaaaaataa atgcagttag tacgcttata acaaggaata gcgagcatca
      901 ttaatgaaca aaatatttaa agttatctgg aaccctgcga cagggaatta tactgttacc
      961 agcgaaacgg caaaaagccg tggcaagaaa tctgggcgca gtaagctgtt aatttctgcg
     1021 ctggttgcgg gtggaatgtt gtcgtcgttt ggggcattgg cgaatgccgg gaatgacaac
     1081 ggccagggtg ttgattacgg tagtggatca gctggcgaca gctgggttgc tataggcaaa
     1141 ggggcgaaag caaatacttt tatgaacacc agtggttcca gtactgctgt gggttatgac
     1201 gctatagctg aaggccaata tagctctgcc atcgggtcaa aaacccatgc gattggtggt
     1261 gcatcaatgg cctttggggt tagtgcaata tcagaaggcg atagaagtat agcactgggt
     1321 gcctcttcgt attcattggg ccaatactca atggccctcg gccgttattc aaaagcattg
     1381 ggtaaattgt ctattgctat gggggactct tccaaagcgg aaggagcaaa cgccattgcc
     1441 ctgggaaatg ccactaaagc tactgagatt atgagtattg ctcttggcga caccgccaat
     1501 gcgtcaaaag cgtattcaat ggcgctggga gcaagtagcg tcgcatctga agaaaacgca
     1561 attgccctgg ggcgtagcag tgtagctagc ggtactgaca gcctcgcatt tggcagacaa
     1621 tcacttgcca gcgcagcgaa cgctattgcg ataggtgctg agaccgaagc cgctgaaaat
     1681 gcaactgcta ttggcaataa tgcgaaggca aaagggacta atagcatggc aatggggttc
     1741 ggaagccttg ccgataaagt caatactatc gcattaggaa atggcagcca ggctctggca
     1801 gataatgcaa tcgccatagg ccagggcaac aaagctgatg gcgtggatgc catcgctctg
     1861 ggtaatggta gccagtcgag aggcttaaac accattgcct taggcacagc cagtaatgca
     1921 actggtgata agagtcttgc gcttggtagt aatagcagtg ccaacggtat taactctgtc
     1981 gcgctgggcg cagattccat tgcggattta gacaataccg tctctgtcgg caatagttca
     2041 ttaaaacgca agatcgttaa tgtgaaaaat ggcgcgatca agtctgacag ttacgatgcc
     2101 attaatggtt cacagcttta tgccattagc gactcggtag caaaaaggct tggaggaggg
     2161 gctgcagtag atgttgatga cggtactgtt acagcaccaa cctacaattt aaaaaatggt
     2221 agcaaaaata acgtaggggc tgcgctcgct gtacttgatg aaaacaccct gcaatgggac
     2281 caaaccaaag gcaaatacag cgctgctcat ggtactagta gcccaactgc cagcgtaatc
     2341 accgatgttg cggatggcac gatttcagcc tccagtaagg atgcggttaa cggttcccaa
     2401 ctgaaagcta ccaatgacga tgtcgaagcc aacaccgcca atatcgctac taataccagc
     2461 aacattgcca cgaatacagc aagtattgcc accaatacca ccaatatcac caacctgacg
     2521 gattccgttg gtgaccttca ggctgatgcc ctgctctgga acgaaactaa aaaggcattc
     2581 agtgcagctc acggccagga taccaccagc aaaatcacca acgttaaaga tgccgacctg
     2641 acggctgaca gcactgatgc tgttaacggc tctcagctga aaaccaccaa cgatgctgtg
     2701 gcgacgaata ccaccaatat cgccaataac acttccaata ttgccactaa caccaccaac
     2761 atctctaatc tgactgagac ggtgactaat cttggtgagg atgcgctgaa atgggataag
     2821 gacaatggtg tcttcactgc agctcatggc aacaataccg ccagcaaaat caccaatatc
     2881 ctggacggca cagtcactgc aaccagttcc gatgccatta acggtagcca gctttatgac
     2941 ttaagcagca atatcgccac ctacttcggc ggcaatgctt ctgtgaatac tgacggtgtg
     3001 tttaccggtc caacctacaa aatcggtgaa acaaattatt ataacgtcgg cgatgcactg
     3061 gctgcgatta actcctcatt tagcacgtct ctcggcgatg ctctgctttg ggatgccacc
     3121 gcaggtaaat tcagtgccaa acacggtact aatggtgacg caagcgtgat cactgatgtc
     3181 gcagatggtg aaatttcaga ctccagttct gacgcagtaa acggctcaca actccacggc
     3241 gtgagcagtt atgttgttga tgcgctgggg ggtggtgccg aagtcaatgc agacggcacc
     3301 atcactgcgc cgacgtacac cattgctaat gctgattacg ataatgtcgg tgatgccctg
     3361 aatgctatcg ataccactct tgacgacgct ctgctctggg atgcggacgc cggtgaaaat
     3421 ggtgcattta gcgccgctca cggaaaagat aaaactgcca gtgtaatcac taacgtcgct
     3481 aacggtgcaa tctctgctgc cagcagcgac gcgattaacg gctcacaact ctataccacc
     3541 aataagtaca tcgctgatgc gctgggtggt gacgcagaag tcaacgctga cggcaccatc
     3601 accgcaccga cttacaccat tgcgaacgcc gagtacaaca acgtcggtga cgccctggat
     3661 gcgcttgatg ataacgccct gctgtgggat gagactgcca atggcggtgc tggagcctac
     3721 aatgccagcc atgacggtaa agccagcatc atcactaatg tcgctaatgg cagtattagt
     3781 gaggacagta ccgatgcagt gaacggttct cagttgaatg cgacgaatat gatgattgag
     3841 cagaacaccc aaattatcaa tcagctcgct ggtaacaccg acgcaaccta tatcgaagaa
     3901 aatggcgctg gtatcaacta cgttcgtact aacgacaacg atttagcctt taacgatgca
     3961 agcgcctctg gtgtcggcgc tacagctgta ggttataacg ctgtcgcgtc tggtgccagc
     4021 agcgtagcca ttggtcagaa cagcagcagc accgttgata ccggtattgc gctgggtagc
     4081 agctccgttt ccagccgtgt gattgccaaa ggttctcgtg acactagcgt aacggaaaat
     4141 ggcgtggtga ttggttatga caccactgac ggcgaactgc taggtgcatt gtcaattggt
     4201 gatgacggta aataccgcca aatcatcaac gtagctgatg gttcagaagc ccatgacgcc
     4261 gttacggttc gccagttgca gaacgctatt ggagcggtcg ccactacgcc aaccaagtac
     4321 tttcacgcca actcaacggc agaagactca ctggccgttg gtgaagactc gctggcaatg
     4381 ggtgcgaaaa ctgtcgttaa tggtaatgca ggtattggta tcggtttgaa cactctggtt
     4441 ctagctgatg cgatcaacgg cattgctatc ggcagtaacg cacgcgcaaa ccatgcaaac
     4501 agtatcgcaa tgggtaacgg ttctcagacc acccgtggtg cacagactgg ctacaccgcc
     4561 tacaacatgg acgcaccgca gaactctgta ggtgagtttt ctgtcggtag tgaagacggt
     4621 caacgtcaga tcacaaacgt cgcagctggt tcagcggata ccgatgcggt taacgtgggt
     4681 cagttgaaag tcactgatga gcgcgtagcg caaaataccc agagcattac taacctgaac
     4741 aatcaggtca ctaatctgga tactcgcgtt actaatatcg aaaacggtat tggcgacatt
     4801 gtcaccaccg gtagcaccaa gtacttcaag accaacaccg atggcgtaga tgccaacgcc
     4861 cagggtaaag atagcgttgc tattggttct ggttccattg ctgccgctga caacagcgtc
     4921 gcactgggta ccggttccgt tgcagaggaa gaaaatacaa tctctgtagg ttcttctact
     4981 aaccaacgcc gtatcaccaa cgtagctgcc ggtgttaatg ccaccgatgc ggttaacgtg
     5041 tcgcagctga aatcttctga agcaggcggc gtgcgctacg acaccaaagc tgatggttct
     5101 atcgactata gcaatatcac cctcggtggt ggcaacggca gtacgactcg tatcagcaac
     5161 gtctccgctg gcgtcaacaa caacgacgcg gtgaactacg cgcagttgaa gcaaagcgtg
     5221 caggaaacga agcaatacac cgatcagcgg atggttgaga tggataacaa actgtctaaa
     5281 actgaaagca agttgagcgg tggtatcgct tctgcaatgg caatgaccgg tctgccgcag
     5341 gcttatacac cgggtgccag catggcttct attggtggcg gtacttacaa cggtgaatcg
     5401 gcagttgctt taggtgtatc gatggtgagc gccaatggtc gttgggtcta caaattacaa
     5461 ggtagtacca atagccaggg tgaatactcc gccgcactcg gtgccggtat tcagtggtaa
     5521 tgatccatta agttagtgtg actaagggca aacgccaaat tgcctgatgc gttacgcgca
     5581 tcaggcaaga taaggcgccc cgggtcaaca atctaagggc aattctctga tgaggattgc
     5641 ccttttcttt accagacatc tccccccaca agaattggcc ctaccaattc ttcgcttatc
     5701 tgacctctgg ttcacaattc cccaattaaa actcacatca atattgccaa tacataacat
     5761 ttagttaacc attcattgtc attatcccta cacaacacaa ttggcagtgc cacttttaca
     5821 caacgtgtga caaggagatg agcaacagac tcattacacg atgtgcgtgg actccaggag
     5881 acctgcaatg aatctctggc aacaaaacta cgatcccgcc gggaatatct ggctttccag
     5941 tctgatagca tcgcttccca tcctgttttt cttctttgcg ctgattaagc tcaaactgaa
     6001 aggatacgtc gccgcctcat ggacggtagc aatcgccctt gccgtggctt tgctgttcta
     6061 taaaatgccg gtcgctaacg cgctggcctc ggtggtttat ggcttcttct atggtttgtg
     6121 gcccatcgcg tggatcatta ttgcagcagt gttcgtctat aagatctcgg tgaaaaccgg
     6181 gcagtttgac atcattcgct cgtctattct ttcgataacc cctgaccagc gtctgcaaat
     6241 gctgatcgtc ggtttctgtt tcggcgcttt ccttgaagga gccgcaggct ttggcgcacc
     6301 ggtagcaatt accgccgcat tgctggtcgg cctgggtttt aaaccgctgt acgccgccgg
     6361 actgtgcctg attgttaaca ccgcgccagt ggcatttggc gcgatgggca ttccaatcct
     6421 ggttgccgga caagtaacag gcatcgacag ctttgagatt ggccagatgg tggggcggca
     6481 gctaccgttt atgaccatta tcgtgctgtt ctggatcatg gcgattatgg acggctggcg
     6541 cggtatcaag gagacgtggc ctgcggtcgt ggttgcgggc ggctcgtttg ccatcgctca
     6601 gtaccttagc tctaacttca ttgggccgga gctgccggac attatctctt cgctggtatc
     6661 actgctctgc ctgacgctgt tcctcaaacg ctggcagcca gtgcgcgtat tccgttttgg
     6721 tgatttgggg gcgtcaaaga ttgatatgac gctggcccac accggttaca ctgcgggtca
     6781 ggtgttacgt gcctggacac cgttcctgtt cctgacagct accgtaacac tgtggagcat
     6841 cccgccgttt aaagccctgt tcgcatcggg tggtgcgctg tatgagtggg tgatcaacat
     6901 tccggtgccg tacctcgata aactggttgc ccgtatgcca ccagtggtca gcgaggctac
     6961 agcctatgcc gccgtgttta agtttgactg gttctctgcc accggcaccg ccattctgtt
     7021 tgctgcactg ctctcgattg tctggctgaa gatgaaaccg tctgacgcta tcagcacctt
     7081 cggcagcacg ctgaaagaac tggctctacc catctactcc atcggcatgg tgctggcgtt
     7141 cgcctttatc tcgaactatt ccggactgtc atcaacactg gcactggcac tggcgcacac
     7201 cggtcatgca ttcaccttct tctcgccgtt cctcggctgg ctgggggtat tcctgaccgg
     7261 gtcggatacc tcatctaacg ccctgttcgc cgcgctgcaa gccaccgcag cacaacaaat
     7321 tggcgtctct gatctgttgc tggttgccgc caataccacc ggtggcgtca ccggtaagat
     7381 gatctccccg caatctatcg ctatcgcctg tgcggcggta ggcctggtgg gcaaagagtc
     7441 tgatttgttc cgctttactg tcaaacacag cctgatcttc acctgtatgg tgggcgtgat
     7501 caccacgctt caggcttatg tcttaacgtg gatgattcct taatgattgt tttacccaga
     7561 cgcctgtcag acgaggttgc cgatcgtgtg cgggcgctga ttgatgaaaa aaacctggaa
     7621 gcgggcatga agttgcccgc tgagcgccaa ctggcgatgc aactcggcgt atcacgtaat
     7681 tcactgcgcg aggcgctggc aaaactggtg agcgaaggcg tgctgctcag tcgacgcggc
     7741 ggcgggacgt ttattcgctg gcgtcatgac acatggtcgg agcaaaacat cgtccagccg
     7801 ctaaaaacgc tgatggccga tgatccggat tacagtttcg atattctgga agcccgctac
     7861 gccattgaag ccagcaccgc atggcatgcg gcaatgcgcg ccacacctgg cgacaaagaa
     7921 aagattcagc tttgctttga agcaacgcta agtgaagacc cggatatcgc ctcacaagcg
     7981 gacgttcgtt ttcatctggc gattgccgaa gcctcacata acatcgtgct gctgcaaacc
     8041 atgcgcggtt tcttcgatgt cctgcaatcc tcagtgaagc atagccgtca gcggatgtat
     8101 ctggtgccac cggttttttc acaactgacc gaacaacatc aggctgtcat tgacgccatt
     8161 tttgctggtg atgccgacgg ggcgcgtaaa gcaatgatgg cgcaccttag ttttgttcac
     8221 accaccatga aacgattcga tgaagatcag gctcgccacg cacggattac ccgcctgccc
     8281 ggtgagcata atgagcattc gagggagaaa aacgcatgat tatttccgca gccagcgatt
     8341 atcgcgccgc agcgcaacgc attctgccgc cgttcctgtt ccactatatg gatggtggtg
     8401 catattctga atacacgctg cgccgcaacg tggaagattt gtcagaagtg gcactgcgcc
     8461 agcgtattct gaaaaacatg tccgacttaa gcctggaaac gacgctgttt aatgagaaat
     8521 tgtcgatgcc ggtagcactg gcaccggtgg gtttgtgtgg catgtatgcg cgtcgtggcg
     8581 aagttcaggc agccaaagcg gcggacgcgc atggcattcc gtttactctc tcgacggttt
     8641 ccgtttgccc gattgaagaa gtcgcgccag ccatcaagcg cccaatgtgg ttccagcttt
     8701 atgtactgcg cgatcgcggc tttatgcgta acgcgctgga gcgagcaaaa gcagcgggtt
     8761 gttcgacgct ggttttcacc gtggatatgc cgacaccggg cgcacgctac cgtgatgcgc
     8821 attcaggtat gagcggccca aatgcggcaa tgcgccgcta cttgcaggcg gtgacgcatc
     8881 cgcaatgggc gtgggatgtg ggcctgaacg gtcgtccaca tgatttaggt aatatctcag
     8941 cttatctcgg caaaccgacc ggactggaag attacatcgg ctggctgggg aataacttcg
     9001 atccgtccat ctcatggaaa gaccttgagt ggatccgcga tttctgggat ggcccgatgg
     9061 tgatcaaagg gatcctcgat ccggaagatg cgcgcgatgc agtacgtttt ggtgctgatg
     9121 gaattgtggt ttctaaccac ggtggccgcc agctggacgg tgtactctct tccgctcgtg
     9181 cgttgcccgc tattgcagat gcggtgaaag gtgatatcgc cattctggcg gatagcggaa
     9241 ttcgtaacgg gcttgatgtc gtgcgtatga ttgcgctcgg tgccgacacc gtactgctgg
     9301 gtcgtgcttt cctgtatgcg ctggcaacag cgggccaggc gggtgtagct aacctgctaa
     9361 atctgatcga aaaagagatg aaagtggcga tgacgctgac tggcgcgaaa tcgatcagtg
     9421 aaattacgca agattcgctg gtgcaggggc tgggtaaaga gttgcctacg gcactggctc
     9481 cgatggcgaa agggaatgcg gcatagtcgt ttgcgccccc tcaccctaac cctctccctc
     9541 agggagaggg gaccgttcgg cgctgtatgt actccctcac tctgaaacga caccgcactc
     9601 tttttttctc cctcgcccct ccggggaggg ccggggtgag gggaaaaggc cgcacggttc
     9661 accaattttc tgctatcctg cccccgcact aagggggcag tatgctaaac atcgtacttt
     9721 acgaaccaga aattccgcca aatactggca acatcatccg tctttgcgct aataccggct
     9781 ttcgtctgca tatcatcgaa ccgatgggat ttgcctggga cgataagcgc ctgcgccgcg
     9841 cggggctgga ctatcacgag tttaccgccg ttacgcgtca tcatgactat cgcgcgttcc
     9901 tcgaagcaga aaatccccag cgcctgtttg ccctcaccac gaaaggtacg cctgctcaca
     9961 gcgccgtaag ctatcaggat ggcgattatc tgatgttcgg cccggaaaca cgcggcctgc
    10021 cagcgagcat tcttgatgcc ctgcccgctg aacaaaaaat tcgcattccg atggtgccgg
    10081 acagccgcag catgaatctg tccaatgcgg tgtcggtagt ggtgtatgaa gcctggcggc
    10141 agctgggata tccgggagcg ttgttgagag attagttact gtatgccgga tagatgatta
    10201 catcgcatcc ggcacgatca caggacatta gatcccatcc ccatactcaa atgtatggtt
    10261 aataccgttg aaatgctggt ccatatccat tgatggctta tcgctgtctg gtttaccgac
    10321 aatacgagcc ggaacgccag cggcggttgt atgcggcggc accggttgca gcaccacgga
    10381 acctgcgcca atcttcgcgc cgcgaccaac ttcaatattg ccgaggattt tcgcgcccgc
    10441 gccaatcatc acaccttcac gaattttcgg gtgacggtca ccaccagatt tacccgtacc
    10501 gccaagcgtc acagattgca gaatcgatac gtcgttttca attaccgccg tttcacccac
    10561 aacgatgccc gtggcgtggt cgagcataat gccacggcca atttttgctg ccgggtgaat
    10621 atcgacctgg aacgtcacag aaacctggtt ttgcagaaag attgccaatg cgcgacgccc
    10681 ctgattccac aaccagtgac cgatgcgata ggcctgcaag gcatgaaaac ccttcaggta
    10741 taacaacggg gttgagtatt tatcgactgc cgggtcgcgg gtacgcaccg cctgaatatc
    10801 acaggccgca gaggcgatca tttccgggtc agcggcgtag gcttcttcca ccacttcacg
    10861 gatagcaata gcaggcataa ttggcgatga cagcttgttc gccagcatgt agctcagtgc
    10921 actaccaagg ttttcgtgct tgagtagcgt cgcgtggtaa aaactggcca gcattggctc
    10981 acagtccgcc agcgttctgg cttcggcttt aatattgttc cagacaattt ccagttcttc
    11041 acacgacatt gcttactcca cacgatgaga taatgaccgg cccgttctgc gcgggccggg
    11101 tcatagcggt aacaaaggtt ccctggggtt agtggctgct gcgctcgtcc ttgcgtgcac
    11161 gacctaataa cgtcaatgct gcctcgcgcg cgttttttcc gcaatataat acttgataaa
    11221 tttcctcggt tattggcatt tctacgccga agcgatgcgc cagttcgcgg acttctttcg
    11281 tattgcggta gccttccacc acctgaccaa tcttctcctg cgcgctttgt acatccatgc
    11341 cctgaccgag catcatgcca aaacggcggt tacgcgactg gttgtcggta caggtaagca
    11401 ccagatcgcc cagccccgcc atgcccataa aggtggcagg atcggcaccc agcgccgcgc
    11461 caagacgcga catttcagcc agcccacggg tgatcagtgc cgtacgcgca ttcgcaccaa
    11521 aaccgatacc gtcggacatc cccgcgccaa tggcaatgac gtttttcacc gcaccgccaa
    11581 gctgcacacc gatgaaatcc gggttgctgt aaacgcggaa acttttgcca cagtgcagca
    11641 attgttggag atcatcggca aaggtctggt cggtagaggc caacgaaata gccgtcggta
    11701 aacctgccgc cagttctttc gcaaacgttg ggccagagat aaccgccagc ggaatttgat
    11761 cgcctaaggc ctcacgcgcc acgtcctgta acagacgtcc ggtttccgct tccagccctt
    11821 tggtcgccca caccagacgc gcatcaggac gcatcagcgg tttaatctgg cgcagcactt
    11881 caccaaagac atggctgggt acgacgacga gaatattacg gctggctgcc agcgcagtgg
    11941 cgagatcgct ttcaagatgg agcgtatcgg gaaaaggcac atcggggaga aacgcggcgt
    12001 tacagcggtc gcgttcaagc gttgcgatat gttcagggtc atggccccag aggacaacct
    12061 cgtggccatt tcttgccagg gtaatggcaa gagcggtgcc gtacgagccg gcaccgatca
    12121 cagtcattga agcattacgt tggttcatca ggcatcctga tgttcttcag taccttcgcc
    12181 agcctgctgc tgcaaatagt tcatgaacag cgcatcgaag ttaatcggcg caaggttcag
    12241 ttgcgggaat gtaccgcggg ataccacgct ggtgatgcac tcacgagcat acgggaacag
    12301 aatgttcggg cagtatgcgc ccaggcaatg tgccatctgg gtgccttcga tacccgcgat
    12361 ggagaaaata ccgccctgct gaacttcaca caggaacgcg gtttcttcgc ccagggaggc
    12421 cgttacggta acacgcagta ccacttcgta tacgtcatct gccagttggg aagatgccgt
    12481 atccagatca agtttaactt ctggttgcca atctttctgg aaaacgtgcg gcgcgttcgg
    12541 cgcttcgaaa gagatatcct tggtataaat acgttggatc tggaaagtca tttcagtgtt
    12601 gttttgttct gacatgtgta gaaaaccctt aagtgttgtc cttaaatact gcgcaatgta
    12661 atgccaacgt tcacacgtta tttcagcagg ggatccagtc caccacgtgc atccaatgca
    12721 tacaagtcat cacagccgcc aatgtgctgt gcgtcaataa aaatctgggg aaccgtggtg
    12781 cgaccgctgc gtttgatcat ctcttcacgc ttggcggcat tgccatcgat cggcaactcc
    12841 tgaaaactca cgcccttgct gctcagcagt gcttttgcac gatggcaata cgggcaggtt
    12901 tctttggtat agatttcaac attggccata atttagctcc tgttttttta ccctgaaatt
    12961 tcatgttgca gggcggctgc aaaatggagt gtccccagaa gcttataaca ataagtcgct
    13021 gggggcctaa ttgcagctaa cggcctgcat catgaaagac gacaggtaaa ttatttgccg
    13081 cgcaccagag gcaagttttc gccagcccag ccagcaacgc cctctttcag cacgaatact
    13141 tgcgcaaaac cagctttcgt cagcgcgttt gcaggctcct ggcactgcat gccagaaccg
    13201 tctaccacga taaccggttt gtctttgtgc ttctcaagct caccaacatt gttggctttg
    13261 atttcgctcg gcaacagatt gatagaacct gcgatatggc ctttacggaa gtcatcacgc
    13321 tgacgtaaat ccacaaccac agcgtcttct ttgttgatca gacgcgtagc ttcaccacga
    13381 gtaatcacct tcactttcga ggtcaggctc ttaaacgtag tcacaagaac cgccaccagt
    13441 aacgcgatcc aggcgatact cagtatggga tgacggccaa caaattgcat aatttcttgc
    13501 atggggggta acaactcccg acgtagtgat taaaaaaacc aggaaaggag tatacctgcg
    13561 cggtgcggca aatacagcca gcgcgttaac tggaatgcaa ttttgcgggg cacgacgaaa
    13621 aaaaagcgca aatctggcat cggcttgccg cgagcggtcg tatattttga tcttcagagg
    13681 ctattttatc gattcagctg tagtaaaatt acgcaaattt tgactcttga gtatgaggtt
    13741 gtcgcaatgt cggtttctaa aaaacctatg gtactggtga ttctggatgg ctatggctat
    13801 cgtgaagaac agcaggataa cgccattttt agtgctaaaa ccccggtaat ggatgcactg
    13861 tgggccaatc gtccgcatac cctgatcgac gcttccggtc tggaagtcgg tctgcctgac
    13921 cgtcagatgg gtaactccga agtaggccac gttaacctgg gtgccggccg catcgtgtat
    13981 caggacctga ctcgtctgga cgttgaaatc aaagatcgcg ctttctttgc taatccagtg
    14041 ctgactggcg cggtagataa agcgaaaaac gcaggtaaag cagtacacat tatgggtctg
    14101 ctctccgcag gcggcgtaca cagccacgaa gatcacatca tggcgatggt agaactggca
    14161 gctgaacgcg gcgcagaaaa aatctacctg cacgcattcc ttgacggtcg cgacaccccg
    14221 ccgcgcagtg ctgaatcctc gctgaaaaaa tttgaagaga aatttgctgc gctgggtaaa
    14281 ggccgcgtag cgtccatcat tggtcgttac tacgcgatgg accgcgataa ccgttgggat
    14341 cgcgttgaaa aagcctatga cctgctgact ctggcgcagg gtgagtttca ggccgatact
    14401 gccgttgctg gtttgcaggc agcttatgct cgcgacgaaa acgacgagtt cgtgaaagcg
    14461 accgttattc gtgcagaagg tcagccagat gcggcaatgg aagacggcga tgcgctgatt
    14521 ttcatgaact tccgtgctga ccgcgcgcgt gaaatcactc gtgcattcgt gaacgctgat
    14581 ttcgatggct tcgcgcgtaa gaaagtggtt aacgtcgatt tcgtgatgct gaccgaatac
    14641 gccgctgaca tcaaaactgc ggttgcttac ccacccgctt ccctggttaa caccttcggc
    14701 gagtggatgg cgaaaaacga caaaactcag ttgcgtattt ccgaaaccga aaaatatgcc
    14761 cacgttactt tcttcttcaa cggtggcgta gaagagtcgt tcaaaggcga agatcgcatt
    14821 ctgatcaact caccgaaagt ggctacctac gatctgcaac cggaaatgag ctccgcagag
    14881 ctgaccgaaa aactggttgc ggccatcaag agcggcaaat acgacaccat tatctgtaac
    14941 tatccgaacg gcgacatggt aggtcacacc ggggtgatgg aagcggcggt taaagcggtt
    15001 gaagcactgg atcactgcgt ggaagaagtc gcgaaagcgg ttgagtccgt gggtggacaa
    15061 ctgctgatca ccgctgacca cggtaacgct gaacagatgc gcgatccggc aacgggtcag
    15121 gcacacacgg cacacaccaa cctgccagtt ccgctgattt acgttggtga taagaacgtg
    15181 aaagcggttg caggcggcaa actttctgac atcgcgccga ccatgttgtc gctgatgggt
    15241 atggaaatcc cgcaagagat gactggtaag ccgctgttca tcgtggaata atccctcccc
    15301 atgaggggaa aggcgattaa taccatgaca cgggccgtga aaccgcgcag gtttgcaatc
    15361 aggcccatca tctacgccag cgttcttagc gctggcgtat tgttgtgcgc cttttccgcc
    15421 cacgcggatg agcgtgacca actcaaatct attcaggccg atatcgccac aaaagagcgc
    15481 gcggtacgcc aaaagcaaca acaacgcgca agcctgctcg cacaattgaa aaagcaggaa
    15541 gaagcgatct ctgaagccac ccgtaagctg cgcgaaacgc aaaacacgct caatcaactc
    15601 aataaacaga ttgatgagat gaacgcgtcg attgccaaac tggagcagca aaaagccgcc
    15661 caggagcgca gcctcgccgc acaactggat gccgcattcc gtcagggcga gcataccggt
    15721 attcagctaa ttctcagcgg tgaagaaagc cagcgtggac agcgtttaca ggcttatttc
    15781 ggctatctca accaggcgcg acaagaaacc attgcccagt tgaagcaaac gcgtgaagaa
    15841 gtcgccatgc agcgtgctga actggaagag aaacaaagcg agcaacaaac gctgttatat
    15901 gaacagcgcg cccaacaggc gaaactgact caggctctga acgagcgtaa aaagacgctg
    15961 gcggggctgg aatcttccat ccagcaaggt cagcagcagc taagtgaact gcgtgccaac
    16021 gaatcccgtc tgcgtaacag tattgcccgt gcggaagccg cggcgaaagc gcgtgctgaa
    16081 cgagaagcgc gtgaggctca ggcagttcgc gacaggcaga aagaagcgac gcgcaaaggc
    16141 accacctaca aaccgaccga aagcgaaaaa tcgctgatgt cccgaactgg tggcctgggt
    16201 gcgccgcgtg gtcaggcatt ctggccagtt cgtggcccta cgctgcatcg ctatggcgag
    16261 cagctacagg gtgaattacg ctggaaagga atggttatcg gtgcctctga aggtactgaa
    16321 gttaaagcaa ttgccgacgg tcgggtgatt ctggctgact ggctgcaagg ctacggtctg
    16381 gtggtggtgg ttgagcacgg taaaggcgat atgagtcttt acggctataa tcaaagcgcg
    16441 ctggtgagcg ttggttcgca ggttcgtgcg ggccagccaa ttgcactggt gggcagcagt
    16501 ggcggtcagg gcagaccttc actctatttc gaaattcgcc gccagggtca ggccgtcaat
    16561 ccacagccgt ggttgggaag ataagttttg tttccatttc gtcgtaacgt tcttgcattt
    16621 gccgctctgt tggcgctctc ctcccctgta attgctggca aacttgccat cgtcattgat
    16681 gattttgggt atcgcccgaa caacgaaaac caggtgctgg cgatgccttc cgctatctcc
    16741 gtcgctgtat tacccgattc accgcacgcc agagagatgg cgaccaaagc gcataacagc
    16801 ggtcacgaag tgttgattca tctcccgatg gcaccgttga gtaaacagcc gctggagaaa
    16861 aatacgctgc gcccggagat gagcagcgac gaaattgagc gcattattcg tagtgcggtc
    16921 aataacgtgc cctatgccgt ggggatcaat aaccacatgg gcagcaagat gacctctaac
    16981 ctgtttggta tgcagaaagt gatgcaggcg ctggagcgtt ataatcttta cttcctcgac
    17041 agcgtaacca ttggtaatac ccaggcgatg cgcgccgcgc aaggcactgg cgtgaaggtg
    17101 atcaaacgga aggtgttcct cgacgattca cagaacgaag cggacatccg tgtgcaattc
    17161 aatcgcgcaa ttgacctagc gcgtcgcaac ggatcaacca ttgccattgg gcatcctcac
    17221 ccttcaacgg tacgcgtgtt gcaacagatg gtttataacc tgccgccaga cattacgctg
    17281 gtgaaagcca gcagcttgct gaatgaaccg caggttgata cgtctacacc gccgaaaaac
    17341 actgtgcccg acacaccgcg taatccgttc cgtggcgtga agctgtgcaa accgaagaaa
    17401 ccgatagaac ctgtttatgc taatcgcttc tttgaagtat taagcgaaag catcagccag
    17461 agcacgctga tcgtttactt ccagcatcag tggcaaggct ggggcaaaca gcctgaagcg
    17521 gcgaagctta acgctagcgt aaattaaagg cgctacgcgt aatgcgatgg tgcgaccgcg
    17581 ttttgtcgcg ccattgccat aagcggaaag actacagtaa cgcctgataa cccaccttta
    17641 cgctgcgcac attggtaatc aggcgcttat aca
//
LOCUS       DADMKF010000077        17448 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.078, whole genome shotgun sequence.
ACCESSION   DADMKF010000077 DADMKF010000000
VERSION     DADMKF010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17448
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.078"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(<1..502)
                     /locus_tag="JNC24_002409"
     CDS             complement(<1..502)
                     /locus_tag="JNC24_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004155169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HAY9159138.1"
                     /translation="MALTDIKVRTAKPTDKQYKLTDGGGMHLLVHPNGSKYWRLQYRY
                     EGKQKMLALGVYPEITLADARIRRDEPRKLLANGVDPGDKKKSDKVEQSKARTFKEVA
                     IEWHGTNKKWSEDHAHRVLKSLEDNLFAALGERNIAELKTRDLLAPIKAVEMSGRLEM
                     AARLQQR"
     gene            complement(688..772)
                     /locus_tag="JNC24_002410"
     tRNA            complement(688..772)
                     /locus_tag="JNC24_002410"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(736..738),aa:Leu,seq:caa)
     gene            968..1987
                     /gene="ahr"
                     /locus_tag="JNC24_002411"
     CDS             968..1987
                     /gene="ahr"
                     /locus_tag="JNC24_002411"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HAY9159139.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYEPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKTLAGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSVSGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRNGKARYRVV
                     LKADF"
     gene            complement(1991..2554)
                     /gene="idnK"
                     /locus_tag="JNC24_002412"
     CDS             complement(1991..2554)
                     /gene="idnK"
                     /locus_tag="JNC24_002412"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HAY9159140.1"
                     /translation="MAGESFILMGVSGSGKTLIGSKVAALLSAKFIDGDDLHPAKNID
                     KMSQGIPLSDEDRLPWLERLNDASYSLYKKNETGFIVCSSLKKQYRDILRKGSPHVHF
                     LWLDGDYETILARMQRRAGHFMPVALLKSQFEALERPQADEQDIVRIDINHDIANVTE
                     QCRQAVLAIRQDRICAKGGSASDQRCE"
     gene            2771..3802
                     /locus_tag="JNC24_002413"
     CDS             2771..3802
                     /locus_tag="JNC24_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-idonate 5-dehydrogenase"
                     /protein_id="HAY9159141.1"
                     /translation="MQVKTQSCVVADKKTVAVTEQTIDWNNNGTLVQITRGGICGSDL
                     HYYQEGKVGNFMIKAPMVLGHEVIGKVIHSDSSELHEGQTVAINPSKPCGHCKYCIEH
                     NENQCTDMRFFGSAMYFPHVDGGFTRYKMVETSQCVPYPAKADEKVMAFAEPLAVAIH
                     AAHQADELQGKRVFVSGVGPIGCLIVSAVKTLGSAEIVCADVSPRSLSLAKEMGADVL
                     VNPQNDDMDHWKAEKGHFDVSFEVSGHPSSVNTCLEVTRARGVMVQVGMGGAMAEFPM
                     MTLIGKEISLKGSFRFTSEFNTAVSWLANGVINPLPLLSAEYLFTDLEEALRFAGDKT
                     QAAKVQLVF"
     gene            3826..4590
                     /gene="idnO"
                     /locus_tag="JNC24_002414"
     CDS             3826..4590
                     /gene="idnO"
                     /locus_tag="JNC24_002414"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HAY9159142.1"
                     /translation="MNDLFSLAGKNILITGSAQGIGYLLATGLGKYGAQIIINDITAE
                     RAELAVEKLHQEGIQAVAAPFNVTHKHEIDAAVEHIEKDIGPIDVLVNNAGIQRRHPF
                     TEFPEQEWNDVIAVNQTAVFLVSQAVTRHMVERKAGKVINICSMQSELGRDTITPYAA
                     SKGAVKMLTRGMCVELARHNIQVNGIAPGYFKTEMTKALVEDEAFTAWLCKRTPAARW
                     GDPQELIGAAVFLSSKASDFVNGHLLFVDGGMLVAV"
     gene            4655..5974
                     /gene="idnT"
                     /locus_tag="JNC24_002415"
     CDS             4655..5974
                     /gene="idnT"
                     /locus_tag="JNC24_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gnt-II system L-idonate transporter"
                     /protein_id="HAY9159143.1"
                     /translation="MPLIIIAAGVALLLILMIGFKVNGFIALVLVAAVVGFAEGMDAQ
                     AVLHSIQNGIGSTLGGLAMILGFGAMLGKLISDTGAAQRIATTLIATFGKKRVQWALV
                     ITGLVVGLAMFFEVGFVLLLPLVFTIVASSGLPLLYVGVPMVAALSVTHCFLPPHPGP
                     TAIATIFEANLGTTLLYGFIITIPTVIVAGPLFSKLLTRFEKAPPEGLFNPHLFSEEE
                     MPSFWNSIFAAVIPVILMAIAAVCEITLPKTNTVRLFFEFVGNPAVALFIAIVIAIFT
                     LGRRNGRTIEQIMDIIGDSIGAIAMIVFIIAGGGAFKQVLVDSGVGQYISHLMTGTTL
                     SPLLMCWTVAALLRIALGSATVAAITTAGVVLPIINVTHADPALMVLATGAGSVIASH
                     VNDPGFWLFKGYFNLTVGETLRTWTVMETLISIMGLLGVLAINAVLH"
     gene            6041..7039
                     /gene="idnR"
                     /locus_tag="JNC24_002416"
                     /gene_synonym="gntH"
                     /gene_synonym="yjgS"
     CDS             6041..7039
                     /gene="idnR"
                     /locus_tag="JNC24_002416"
                     /gene_synonym="gntH"
                     /gene_synonym="yjgS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator IdnR"
                     /protein_id="HAY9159144.1"
                     /translation="MRNHRISLQDIATLAGVTKMTVSRYIRSPKKVAKETGERIAKIM
                     EEINYIPNRAPGMLLNAQSYTLGILIPSFQNQLFADILAGIESVTSVHNYQTLIANYN
                     YDRDSEEESVINLLSYNIDGIILSEKYHTIRTVKFLRSATIPVVELMDVQGERLDMEV
                     GFDNRQAAFDMVCTMLDKRVRRKILYLGSKDDTRDEQRYQGYCDAMMLHNLSPLRMNP
                     RAISSIHLGMQLMRDALSANPDLDGVFCTNDDIAMGALLLCRERNLAVPEQISIAGFH
                     GLEIGRQMIPSLASVITPRFDIGRMAAQMLLSKIKNNDHNHNTVDLGYQIYHGNTL"
     gene            7117..8619
                     /locus_tag="JNC24_002417"
     CDS             7117..8619
                     /locus_tag="JNC24_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 domain-containing protein"
                     /protein_id="HAY9159145.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLARLKNIGVNDWQPHANPVVVWDIFG
                     EKGHPVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAEHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRAFTPKDQKAV
                     KAAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDEVDRESAYEMLQKGFQASTEQQNNPPVKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRKR"
     gene            complement(8738..9820)
                     /gene="lptG"
                     /locus_tag="JNC24_002418"
     CDS             complement(8738..9820)
                     /gene="lptG"
                     /locus_tag="JNC24_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HAY9159146.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVASQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEELGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTNLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(9820..10920)
                     /gene="lptF"
                     /locus_tag="JNC24_002419"
     CDS             complement(9820..10920)
                     /gene="lptF"
                     /locus_tag="JNC24_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HAY9159147.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVVLDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            11187..12698
                     /gene="pepA"
                     /locus_tag="JNC24_002420"
     CDS             11187..12698
                     /gene="pepA"
                     /locus_tag="JNC24_002420"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HAY9159148.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            12832..13275
                     /gene="holC"
                     /locus_tag="JNC24_002421"
     CDS             12832..13275
                     /gene="holC"
                     /locus_tag="JNC24_002421"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HAY9159149.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            13275..16130
                     /gene="valS"
                     /locus_tag="JNC24_002422"
     CDS             13275..16130
                     /gene="valS"
                     /locus_tag="JNC24_002422"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HAY9159150.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGADVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(16186..17382)
                     /locus_tag="JNC24_002423"
     CDS             complement(16186..17382)
                     /locus_tag="JNC24_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF898 domain-containing protein"
                     /protein_id="HAY9159151.1"
                     /translation="MAQVINDMDVPSHSFVFHGTGERYFLICVVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQRFSYGITGGNVFVSCLVFVFFYFAILMTVSADMPLIGCV
                     LTLSLLVLLIFMAAKGLRYQALMTSLNGVRFSFNCSMKGFWWVTFFLPILMALGMGTV
                     FFISTKMLHANSSSSVIISVVLMTIVCIVFIGIFNGTLYSLVMSFLWSNTSFGIHRFK
                     VKLDTTYCIKYAILAFLALLPFLAVAGYIIFDQILNEYDSSGYANDDIENLQQFMEMQ
                     RKMIIAQLIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCAL
                     VVISGITGGLAYPLLKIWMIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLASISRGIM
                     PSLPFL"
BASE COUNT         4484 a         4335 c         4453 g         4176 t
ORIGIN      
        1 tgcgctgttg aagacgagca gccatttcaa gacgtccaga catttctacg gccttaatag
       61 gtgctaataa atctcgagtt tttaactcag cgatattacg ttcaccaagc gctgcaaaaa
      121 gattatcttc cagacttttt agcacacgat gggcatgatc ttcagaccac tttttattgg
      181 tgccatgcca ctcaatcgcg acttctttaa aggttcgtgc tttactctgt tcaaccttat
      241 cacttttctt tttgtctccc ggatcgacgc cattcgcaag cagcttacgt ggctcgtcac
      301 gacgtattct ggcatccgcg agtgtgattt caggataaac cccaagtgcc agcatctttt
      361 gctttccctc ataacggtac tgcaaacgcc agtacttaga accatttgga tggacaagca
      421 gatgcatacc gccaccatca gtcaacttat attgcttatc tgttggctta gctgttctga
      481 ctttgatgtc tgttaatgcc atgcgtattc cctccctgtt ggtacaagca ttatcgaacc
      541 ggaaatacca tcatctgtac caacatatgt tggtagaagt acgttgatat cggttgacct
      601 tgagagagtt aatatagcgg gaaaggtaga taaatactga atttcaggca taaaaaagac
      661 ctcagttgag gtctattgac atacttttgg tgccgaaggc cggactcgaa ccggcacgta
      721 tttctacggt tgattttgaa tcaactgcgt ctaccgattt cgccacttcg gcactgaaga
      781 ggtatgcgga aaacgttgtg gattatacct gtcgcacgcc accatgcaag cgccagcttg
      841 ctcgcacctc gctaagtgct gaaattttca gcattgcata cagcgatgtg taacctttgt
      901 cacactccag gcaccccgcc ctgccatgct ctacacttcc cagacaacac cagagaagga
      961 ccaaaaaatg tcgatgataa aaagctatgc cgcaaaagaa gcgggcggcg aactggaagt
     1021 ttatgagtac gagcccggtg aattgaagcc acaagatgtt gaagtgcagg tggattactg
     1081 cgggatctgc cattccgatc tgtcgatgat cgacaacgaa tggggatttt cacagtatcc
     1141 gctggttgcc gggcatgagg tgattgggcg cgtggtggcg ctcgggagcg ccgcgcagga
     1201 taaaggtttg caggtcggtc agcgtgtcgg gattggctgg acggcgcgta gctgcgggca
     1261 ttgcgacgcc tgtattagcg gtaatcagat caactgcgag caaggtgcgg tgccaacaat
     1321 tatgaatcgc ggcggttttg ccgagaagtt gcgtgcagac tggcaatggg tgatcccact
     1381 gccggaaaat atcgatatcg agtccgccgg gccgctgctg tgtggtggta tcacggtctt
     1441 taaaccactg ttgatgcacc atatcactgc taccagccgc gttggggtaa ttggtattgg
     1501 cgggctgggg catatcgcta taaaacttct gcacgcaatg ggatgcgagg tgacagcctt
     1561 tagttcgaat cctgcgaaag agcaggaagt actggcgatg ggtgccgata aagtggtgaa
     1621 tagccgcgat ccgcaggcac tgaaaacact tgcggggcag ttcgatctca ttatcaacac
     1681 cgtcaacgtc agcctcgact ggcagcctta ttttgaggcg ctgacgtatg gcggtaattt
     1741 ccacaccgtc ggtgcggtcc tcacgccgct gtctgttccg gcctttacgt taattgcggg
     1801 cgaccgcagc gtctctggtt cagcaaccgg cacaccttat gagcttcgta agctgatgcg
     1861 ctttgcggcc cgcagcaagg ttgcgccgac caccgaactg ttcccgatgt cgaaaataaa
     1921 cgacgccatc cagcatgtgc gcaacggtaa ggcgcgttac cgcgtggttc tgaaagccga
     1981 tttttgaaaa tcattcgcag cgctgatctg aggcgctacc ccctttcgca catattctgt
     2041 cttgtcgtat cgccagcacg gcctgccgac attgttcggt gacattggca atatcatggt
     2101 tgatatcaat gcgtacaata tcctgttcat ctgcttgtgg acgctccagc gcctcaaact
     2161 gactttttag taacgctacc ggcataaaat gcccagcccg ccgctgcatt cgcgcgagaa
     2221 tagtttcata gtcgccatct aaccagagga aatgaacatg ggggctaccc ttgcgtaaaa
     2281 tatcacgata ctgttttttt aatgatgaac agacaataaa tcctgtttca ttctttttat
     2341 aaagactgta tgaagcatca tttaagcgtt ccagccaggg aagtcgatct tcatcagata
     2401 atggaatacc ctgcgacatt ttatctatat ttttggctgg atgaagatcg tcaccatcaa
     2461 taaatttagc agataataac gcggcaacct tgctaccaat taatgtttta ccactccctg
     2521 aaacgcccat caaaataaag ctttcacccg ccatttaaaa tctcctgaat catcttacct
     2581 gctgcgatac tacgcctggt ggcataataa tttcacgttt ttttatttct gctgataaga
     2641 attacaaggc acatcacgtt atgcgtaaca tagtaatgta acaattttct gacgtgatct
     2701 tcatcacaaa taatgacaat taaaaccgct taaatgcttc cgaggtgtct acctgaccag
     2761 tgaggaattt atgcaagtga aaacacagtc ctgcgttgtt gcggacaaga aaactgttgc
     2821 cgttaccgag cagacgatag attggaataa taatggaaca ttagtacaaa taacccgagg
     2881 tggaatttgc ggttccgatt tacattatta tcaggaagga aaagtaggta atttcatgat
     2941 aaaggcaccg atggtgttag gtcatgaagt tatcggtaaa gttattcata gcgactcatc
     3001 agaattacat gaagggcaaa cggtagccat taatccgtct aaaccgtgcg gtcactgcaa
     3061 atactgcatt gaacataacg agaatcagtg tacagatatg cgtttttttg gcagtgccat
     3121 gtatttccct catgttgatg gtggttttac ccgttataaa atggtcgaaa cgtcgcaatg
     3181 tgtcccttat ccggccaaag ctgacgaaaa ggttatggct tttgccgaac ctttagccgt
     3241 cgcgattcat gccgcacatc aggccgacga gttacagggc aagcgagtat ttgtttccgg
     3301 tgttgggccc attggctgcc tgattgtcag tgctgtgaaa acactggggt ccgcggaaat
     3361 tgtctgtgcc gatgtgagtc cccgttccct ttcgctggcc aaagagatgg gtgcggatgt
     3421 gctcgtaaac ccacaaaacg acgacatgga tcactggaaa gcggaaaaag gccatttcga
     3481 tgtcagcttt gaagtgtccg gtcatccttc atcagtgaat acctgtctgg aggtcactcg
     3541 tgcacgcggc gtaatggtgc aggtagggat gggaggcgcg atggcagaat tcccaatgat
     3601 gacgttgatt ggtaaggaga tttcactcaa aggctctttc cgttttacca gcgaatttaa
     3661 taccgcagtg tcatggctgg cgaatggcgt tatcaatcca ctgcctttac tgagtgctga
     3721 atatctcttc actgacctgg aagaggcgct acgtttcgcc ggtgataaaa cccaggcagc
     3781 aaaagtccag cttgttttct aagagataaa taaaggaata atacaatgaa cgatctattt
     3841 tcactggcag gaaaaaatat cttgattacc ggttcagcac agggcattgg ctatttactg
     3901 gcaaccggcc tgggtaaata tggcgcacaa ataattatta atgatattac tgccgaacgc
     3961 gcagaacttg ctgtagaaaa actccaccag gagggtattc aggccgttgc cgcacctttt
     4021 aatgttactc ataaacatga aattgatgcc gccgttgaac atatcgaaaa ggacatcggc
     4081 cccattgatg tgctggtgaa taacgccggt atccagcgcc gtcatccttt tactgagttc
     4141 cctgaacaag agtggaatga tgtgatcgca gtaaaccaga ccgccgtgtt cctggtatcg
     4201 caagcggtaa ctcgtcacat ggttgaacgc aaggcaggta aagttattaa tatttgctcg
     4261 atgcaaagcg aactgggacg tgacaccatc accccttatg ccgcatcgaa aggggcggta
     4321 aaaatgctca cccgcggcat gtgcgtcgag ctggcgcgcc acaatattca ggtcaacggt
     4381 attgcgccgg gctatttcaa aacagaaatg actaaagcac tggttgagga cgaagccttc
     4441 accgcctggt tgtgcaaacg gacccccgcc gcacgctggg gagatccgca ggaactgatt
     4501 ggtgctgcgg tgttcctttc ttcaaaagcc tctgatttcg taaacggcca cctgttgttt
     4561 gttgatggcg gcatgttagt ggctgtttaa cagccaccac cctacgtgca gcgcgaaagc
     4621 gctgcttttc tggcactaat aagagataaa gactatgcca ttaatcatta ttgcggcagg
     4681 cgtcgcgctg cttcttatcc tgatgatcgg ctttaaagtt aacggcttta ttgccctcgt
     4741 tctggtagct gccgtcgtcg gatttgccga agggatggat gcacaggccg tcctgcactc
     4801 tatacaaaat ggtatcggca gcacgctcgg agggctggca atgatcctcg gtttcggggc
     4861 catgttaggc aagctgattt ctgatacggg cgcggcacaa cgtatcgcca ctacgctgat
     4921 tgctactttt ggtaaaaaac gcgtgcaatg ggcgctagtg atcaccggtc tggttgtggg
     4981 cctcgccatg ttttttgaag tgggttttgt cctgctgttg ccgttggtat ttaccatcgt
     5041 ggcatcatct ggattacccc tgttgtatgt tggcgtaccg atggtagcag cgctctctgt
     5101 aacccactgt tttctgccgc cacatccagg gcctactgcc atcgcgacta tctttgaggc
     5161 taatctcgga acgactttac tgtatggatt tatcattacc attccgacag ttattgtcgc
     5221 aggaccgctg ttttctaaac tgctaactcg ctttgagaaa gcaccaccgg aaggcttatt
     5281 taatcctcat ctgtttagcg aagaggagat gccctccttc tggaacagta tttttgctgc
     5341 agtgatcccg gtcatcctga tggctatcgc cgccgtttgt gaaattacgt taccgaaaac
     5401 taacaccgtg cgcctcttct ttgaatttgt cggtaaccct gccgttgcgc tgtttattgc
     5461 cattgttatt gcgattttca cactgggccg acgtaatgga cgcaccatcg agcaaatcat
     5521 ggatatcatt ggggattcta taggcgctat cgcgatgatt gtgtttatta tcgctggcgg
     5581 cggcgcgttt aagcaggtat tagtagatag tggtgtcggg cagtatattt cacacttaat
     5641 gaccggaact acgctttcgc cgttattgat gtgctggact gtcgcggcgc tgttgcgtat
     5701 cgctctgggc tctgccaccg tcgcggccat taccaccgcg ggtgtggtgt tgccgattat
     5761 caacgttacc catgccgatc ccgctttaat ggtactggca actggtgcgg gcagcgtgat
     5821 cgcgtcacac gtaaacgacc ctggcttctg gctatttaaa gggtatttta atctgacggt
     5881 tggtgaaacg ttgcgtacct ggacagtgat ggaaaccctt atttctatta tgggtttgct
     5941 gggcgtgtta gccattaacg ccgtattgca ctgaaagaat aaaatattgt gatgtagttt
     6001 cgggggaaga ttgaagattc ccccgacgtt gggagaagtt atgcgcaatc acagaatttc
     6061 tttacaggat atcgctacgc tggctggcgt aacaaaaatg accgtgagtc gttatatccg
     6121 ctcgccgaaa aaggtggcaa aggaaacagg cgagcgcatc gccaaaatta tggaagagat
     6181 caattatatc cctaatcgcg ctcccggcat gttgttgaat gcacaaagtt ataccctcgg
     6241 tatcttgatt ccgtcatttc aaaatcaact cttcgctgac attctcgcgg gaatagaatc
     6301 cgtcacctct gtgcataatt atcagaccct tattgctaat tacaattacg atcgcgattc
     6361 cgaagaagag tcggtgatta acctgctctc ttataacatt gacgggatta ttctttcgga
     6421 aaaatatcac accatcagga cggtgaaatt tctgcgttca gccaccattc cggtcgtgga
     6481 attgatggat gtacagggag aacggctgga tatggaggtc ggttttgata atcggcaggc
     6541 tgcttttgac atggtgtgta ccatgctgga taagcgagtc aggcgcaaaa ttctgtatct
     6601 cggttccaaa gatgacaccc gcgatgaaca gcgttatcag gggtattgcg atgcgatgat
     6661 gctgcataat ctttccccat tacgcatgaa tccacgcgcc atctcatcca ttcatttagg
     6721 aatgcagtta atgcgtgatg cccttagcgc aaacccggat ttagacggtg tattttgcac
     6781 caatgacgat attgcgatgg gtgcgcttct gctgtgccgc gagcgaaacc tcgccgtgcc
     6841 agaacaaatt tccattgcgg gttttcatgg gctggagatt ggcaggcaaa tgatcccaag
     6901 cctcgccagc gtaattactc cccgttttga tattggtcgg atggccgccc aaatgttgct
     6961 gagcaagatt aagaataacg atcacaacca caacactgtt gacctcgggt atcagattta
     7021 tcacggtaac accctttagg ttattttctc ttcattacac atattctctt tgccggacgt
     7081 tgcctatact ccaggcagga aactggagga aatctcatga gcgaacccct gttaattgcc
     7141 cgcacgccgg acacagaact gtttttactg ccgggaatgg ctaaccgtca cgggctgatt
     7201 actggcgcaa cggggacggg taaaactgtc acgttgcaaa aactggcaga gtcattgtcg
     7261 gaaatcggtg ttccggtgtt tatggccgat gtgaaaggcg atctgaccgg catcgcgcag
     7321 gcaggaacgg cgtcggaaaa actgctcgca agactgaaaa atatcggcgt caacgactgg
     7381 caaccgcatg ccaatccggt ggtggtgtgg gatatctttg gcgagaaagg ccatccggtg
     7441 cgggcgacgg tttcggatct ggggccgctg ttgctggcac gactgttgaa tctcaacgat
     7501 gtgcaatctg gcgtgctgaa tattatcttc cgcattgcgg acgatcaggg attgttgctg
     7561 ctcgacttta aagatttgcg ggcgattacc cagtacatcg gcgataacgc caaatccttc
     7621 cagaatcagt acggaaatat cagtagcgca tcggttggtg ccatccagcg cggattactg
     7681 tcgctggaac agcaaggcgc agaacacttc tttggtgagc cgatgctgga tatcaaagac
     7741 tggatgcgca ccgatgccaa cggtaaaggc gttatcaata tcctcagcgc cgagaaactt
     7801 tatcagatgc cgaaactgta cgccgccagc ctgctgtgga tgctttcaga gttatatgaa
     7861 caattgcctg aagcgggcga tctggagaaa ccaaaactgg tgtttttctt cgacgaagca
     7921 catctgctgt ttaacgatgc gccacaggta ctgctggata agattgagca ggtgataagg
     7981 cttattcgct caaaaggcgt gggcgtctgg ttcgtttcgc aaaacccgtc agatatcccg
     8041 gataacgtgc tagggcagct cggtaatcgc gttcaacacg ctttgcgtgc ttttacgcct
     8101 aaagatcaga aagcagtgaa ggcagcggcg caaaccatgc gggccaatcc ggcgtttgat
     8161 accgaaaagg caatccagga actggggacc ggcgaggcgt taatctcgtt tctcgatgcg
     8221 aaaggaagtc cttcagtggt ggaacgggcg atggtgatcg caccttgttc gcgaatgggg
     8281 ccggtgacgg aagatgagcg taatggcctg attaatcact ccccggtgta tggcaaatat
     8341 gaagatgagg tggaccgcga gtccgcctat gagatgctgc aaaaaggctt tcaggccagt
     8401 accgagcagc aaaataatcc ccccgtgaaa gggaaagagg tggcggtgga tgacggtatt
     8461 cttggtggat tgaaggatat tttgtttggc actaccggac cacgcggcgg gaagaaagat
     8521 ggtgtggtgc aaacaatggc gaaaagcgcc gctcgccagg tgacgaatca gattgtacgc
     8581 gggatgttgg ggagtttgct gggggggaga aaaaggtaag tcagacgtga tctgtgcccc
     8641 tcaccctaac cctctcccca aaggggcgag gggaccgttc gaactcgtat ttgactttgt
     8701 caacaataat aaagggagct ttcgctccct tgttagatta cgattttctc attaacagcc
     8761 acaggctgat taagaagaag ctggcgcttg gcaacagtgc gccgatgatc ggcgggatgc
     8821 cataaaccaa cgtcagcggg ccgaagatct ggtccagtac gtagaagaca aaaccgaagc
     8881 tgataccggt gaccacacgc acgcccatcg gtacgctacg cagtgggcca aagatgaacg
     8941 acagcgccat caacatcatc accgccacgg atagcggctg gaagattttg ctccacatgt
     9001 tgagctgata acgtccggca tcctgaccgc tcgacttcag atacttcaca tagttgtgca
     9061 aaccactgat ggagagtgca tccgggtcca gcgccaccac gcccagtttg tctggcgtga
     9121 ggttggtttt ccaggtgccg ctcaccgtct gcgaaccggt gatctgtttc ggattggtca
     9181 gatcagattc atcaacctgc gacagacgcc agactttatg ttccgggtca aacttcgcgg
     9241 tagcggcata acgtacggat tgcagacgac gattctcgtt aaaggcataa atactgatgc
     9301 cacctaactc ttcgtcacct ttaacccgct caatgtagac gaagttgttg ccatctttcg
     9361 cccataagcc ttgctgagtg gagagcaacg agccgccgta catcgcctgc gcacggtagt
     9421 tacgcgccat ctgctcgccc tgcgacgcga cccattcacc aatcgccatc gtcagcaaga
     9481 ccagcggaat ggcggttttc atcaccgaca gcgccacctg catacgggta aaaccagaag
     9541 cctgcatcac caccagttcg ctgcgctgcg ccagcatccc aagaccaagc aacgccccaa
     9601 gcagagccgc catcgggaag aagatttgca catctttcgg cacgctcagc aaggtataca
     9661 ttcctgcgcc taacgcgtcg taactcccct gcccggcttt tttcagctga tcgacaaact
     9721 tgataatgcc cgacagcgac accagcatga acagcgtcat catgatggtg gtgaaaatag
     9781 ttttaccgat atagcggtca agtacgccaa aaggttgcat cacaccgctc ctttacgcga
     9841 aaaactggcg cgcaggcggc ggaccggcac ggtgtcccaa aggttgagaa caatcgccaa
     9901 agccagataa atcaggttaa cggtccacat ccacagcgtc gggtccagct tacctttacc
     9961 gccgttcgat ttcagggagg tctggatcag gaagaaaagt agatacagca gcatggctgg
    10021 cagcatcgac agtacgcgtc cctgacgcgg gttaaccacg ctcaacggta caaccataag
    10081 tgccatcata aacacggtga ataccaacgt gatacgccag ttcagttctg cgcgggcacg
    10141 atcggtgtca gtgttccaca atgtgcgcat gtccatctgg tcggtatcgt tcgggtcgag
    10201 caccaccgcc tggtgaccaa tgatcgcctg ataatcctga aagtccgtaa tgcggaaatc
    10261 gcgtaacagt gcagtgcctt cgaagcgcgt tccctggttg agagtgacga cctgggagcc
    10321 gtcgcgcagc tgggttaaat gtccggaatc ggccaccacc acagaaggac gtgcattacc
    10381 ttttggtcga atttgtgcga ggaacacatc tttgaaatcg ctgccgtcaa cgctttcgat
    10441 gaacagcacc gagctgccat tagtcgcttg ctggaattgc ccttgcgcca gcgccgccat
    10501 gccagggttc gctttcgctt ctgctaacac ttcatcctga tgacgcgatg accatggtcc
    10561 cgcccacatc acgttaaccg ccgcgacgat tgccgtgaac accgcaagga tcattgccgc
    10621 tttcaccagc accgctttgc tcagaccgca ggcatgcatt accgtaattt cactttcggt
    10681 atacagtttg cccagcgtca tcagcagccc gaggaacagg cttaatggca ggataagctg
    10741 cgccatttcc ggcacgccca acccgagaag ggagagcacc agattcgccg gaatatcgcc
    10801 gtcaaccgct gcgccgagga tcctcactaa cttttgacag aagaagatca aaagcaagat
    10861 gaagagtatc gccagctggc ttttgagcgt ctcccgcacc agatatctta tgattatcac
    10921 tttaaatacg cccgtaaaaa ctcgtctttt gcaggatttt agcttgtttc atggcttaaa
    10981 cgtcatttat tctcttgagt cgtcgaaatc gtcgctaaga taattatact caacggattc
    11041 acctctcaga ttttgttctg acgtgccaat gccgtaataa cgttaagatt aacacgaagt
    11101 catcgcaaca gcggacatga gttacgaaag cttgcaattc tatctgtagc caccgccgtt
    11161 gtctttaaga ttcaggagcg tagtgcatgg agtttagtgt aaaaagcggt agcccggaga
    11221 aacagcggag tgcctgcatc gtcgtgggcg tcttcgaacc acgtcgcctt tctccgattg
    11281 cagaacagct cgataaaatc agcgatgggt acatcagcgc cctgctacgt cggggcgaac
    11341 tggaaggaaa accggggcag acactgttgc tgcaccatgt tccgaatgta ctttccgagc
    11401 gaattctcct tattggttgc ggcaaagaac gtgagctgga tgaacgtcag tacaagcagg
    11461 ttattcagaa aaccattaat acgctgaatg atactggctc aatggaagcg gtctgctttc
    11521 tgactgaact gcacgttaaa ggccgtaaca actactggaa agtgcgtcag gctgtcgaga
    11581 ctgcaaaaga gacgctctac agtttcgatc agctgaaaac gaacaagagc gaaccgcgtc
    11641 gtccgctgcg taaaatggtg ttcaacgtgc cgacccgccg tgaactgacc agcggtgagc
    11701 gcgcgatcca gcacggtctg gcgattgccg ccgggattaa agcagcaaaa gatctcggca
    11761 atatgccgcc gaatatctgt aacgccgctt acctcgcttc acaagcgcgc cagctggctg
    11821 acagctacag caagaatgtt atcacccgcg ttatcggcga acagcagatg aaagagctgg
    11881 ggatgcactc ttatctggcg gtcggtcagg gttcgcaaaa cgaatcgctg atgtcggtga
    11941 ttgagtacaa aggcaacgcg tcggaagatg cacgcccaat cgtgctggtg ggtaaaggtt
    12001 taaccttcga ctccggcggt atctccatca agccttcaga aggcatggat gagatgaagt
    12061 acgatatgtg cggtgcggca gcggtttacg gcgtgatgcg gatggtcgca gagctacaac
    12121 tgcctattaa cgttattggc gtgctggcag gctgtgaaaa catgcctggc gggcgtgcct
    12181 atcgtccggg cgatgtgtta accaccatgt ccggtcaaac cgttgaagtg ctgaacaccg
    12241 atgccgaagg ccgcctggta ctgtgcgacg tgttaactta cgttgaacgt tttgagccgg
    12301 aagcggtgat tgatgtggcg acgctgaccg gtgcctgcgt gatcgcgctg ggtcatcata
    12361 ttactggtct gatggcgaac cataatccgc tggcccatga actgattgcc gcgtctgaac
    12421 aatccggtga ccgcgcatgg cgcttaccgc tgggtgacga gtatcaggaa caactggagt
    12481 ccaattttgc cgatatggcg aacattggcg gtcgtcctgg tggggcgatt accgcaggtt
    12541 gcttcctgtc acgctttacc cgtaagtaca actgggcgca cctggatatt gcaggaaccg
    12601 cctggcgttc tggtaaagca aaaggcgcaa ccggtcgtcc ggtagcgttg ctggcacagt
    12661 tcctgttaaa ccgcgctggg tttaacggcg aagagtaagt ttgcgtttta ccctcaccct
    12721 aaccctctcc ctaagggaga gggaaccgat cgagcgcaat agaaattcat tgctcatctt
    12781 tcagggtgag gaatgcagca atatttaaat ccaccacaag aagccccata tatgaaaaac
    12841 gcaacgttct accttctgga caatgacacc accgtcgatg gcttaagcgc cgttgagcaa
    12901 ctggtgtgtg aaattgccgc agaacgttgg cgcagcggta agcgcgtgct catcgcctgt
    12961 gaagatgaaa agcaggctta ccggctggat gaagccctgt gggcgcgtcc ggcagaaagc
    13021 tttgtcccgc ataatttagc gggagaagga ccgcgcggcg gtgcgccggt ggagatcgcc
    13081 tggccgcaaa aacgcagcag cagcccgcgc gatatattga ttagtctgcg aacaagcttt
    13141 gcagattttg ccactgcttt cacagaagtg gtagacttcg ttccttatga agattctctg
    13201 aaacaactgg cgcgcgaacg ctataaagcc taccgcgtgg ctggtttcaa cctgaatacg
    13261 gcaacctgga aataatggaa aagacatata acccacaaga tatcgaacag ccgctttacg
    13321 agcactggga aaagcagggc tactttaagc ctaatggcga tgaaagccag gaaagtttct
    13381 gcatcatgat cccgccgccg aacgtcaccg gcagtttgca tatgggtcac gccttccagc
    13441 aaaccatcat ggataccatg atccgctatc agcgcatgca gggcaaaaac accctatggc
    13501 aggtcggtac tgaccacgcc gggatcgcta cccagatggt cgttgagcgc aagattgccg
    13561 cagaagaagg taaaacccgt cacgactacg gccgcgaagc tttcatcgac aaaatctggg
    13621 aatggaaagc ggagtccggc ggcaccatta cccgtcagat gcgccgtctc ggcaactctg
    13681 tcgactggga gcgtgaacgc ttcacgatgg acgaaggcct gtccaatgcg gtgaaagaag
    13741 ttttcgttcg tctgtataaa gaagacctga tttaccgtgg caaacgcctg gtaaactggg
    13801 atccgaaact gcgcaccgct atctctgacc tggaagtgga aaaccgcgaa tcgaaaggtt
    13861 cgatgtggca catccgctat ccgctggctg acggcgcgaa aaccgcagac ggtaaagatt
    13921 atctggtggt tgcgactacc cgtccggaaa ccctgttggg cgataccggc gtggccgtta
    13981 acccggaaga tccgcgttac aaagacctga ttggcaaata tgtcattcta ccgctggtta
    14041 accgtcgtat tccgatcgtt ggcgacgaac acgccgacat ggaaaaaggc accggctgcg
    14101 tgaaaatcac tccggcgcac gactttaacg actatgaagt gggtaaacgt cacgcgctgc
    14161 cgatgatcaa catcctgacc tttgacggcg atatccgtga aagcgcccag gtgttcgata
    14221 ccaaaggtaa cgaatctgac gtttattcca gcgaaatccc ggcagagttc cagaaactcg
    14281 agcgttttgc tgcacgtaaa gcagtcgttg ctgcggttga cgcactcggc ctgctggaag
    14341 aaattaaacc gcacgacctg accgttcctt acggcgaccg tggcggcgta gttatcgaac
    14401 caatgctgac cgaccagtgg tacgtgcgtg ccgatgtact ggcgaaaccg gcggttgaag
    14461 cggttgagaa cggcgacatt cagttcgtac cgaagcagta cgaaaacatg tacttctcct
    14521 ggatgcgcga tattcaggac tggtgtatct ctcgtcagct gtggtggggt caccgtatcc
    14581 ctgcatggta cgacgaagca ggtaacgttt atgttggccg caacgaagaa gaagtgcgca
    14641 aggaaaataa cctcggtgcc gacgttgccc tgcgtcagga cgaagacgtt ctcgacacct
    14701 ggttctcttc tgcgctgtgg accttctcta ccctaggctg gccggaaaac actgacgcac
    14761 tgcgccagtt ccacccaacc agcgtgatgg tatccggctt cgacatcatt ttcttctgga
    14821 ttgcccgcat gatcatgatg accatgcact tcatcaaaga tgaaaatggc aaaccgcagg
    14881 tgccgttcca caccgtttac atgaccggcc tgattcgtga cgacgaaggc cagaagatgt
    14941 ccaaatccaa gggtaacgtt attgacccgc tggatatggt tgacggtatt tcgctgccgg
    15001 aactgctgga gaaacgtacc ggcaatatga tgcagccgca gctggcggac aaaatccgta
    15061 agcgcaccga gaagcagttc ccgaacggta ttgagccgca cggcaccgat gccctgcgct
    15121 tcaccctggc ggcgctggcg tctaccggtc gtgacatcaa ctgggatatg aagcgcctgg
    15181 aaggttaccg taacttctgt aacaagctgt ggaacgccag ccgctttgtg ctgatgaaca
    15241 cggaaggtca ggattgcggc ttcaacggtg gtgaaatgac gctgtcgctg gcggaccgct
    15301 ggattctggc agagttcaac cagaccatca aagcgtaccg cgaagcgctg gacagcttcc
    15361 gcttcgatat cgctgcaggc attctgtatg agttcacctg gaaccagttc tgtgactggt
    15421 atctggagct gactaagccg gtaatgaacg gtggcaccga agccgaactg cgcggtactc
    15481 gccatacgct ggtgaccgta ctggaaggtc tgctgcgcct cgcgcatccg atcattccgt
    15541 tcatcaccga aaccatctgg cagcgtgtga aagtactttg cggtatcact gccgacacca
    15601 tcatgctgca gccgttcccg cagtacgatg catctcaggt tgatgaagcc gcactggccg
    15661 acaccgaatg gctgaaacag gcgatcgttg cggtacgtaa catccgtgca gaaatgaata
    15721 tcgcgccggg caaaccgctg gagctgctgc tgcgtggttg cagcgcggat gcagaacgtc
    15781 gcgtaaatga aaaccgtggc ttcctgcaaa ccctggcgcg tctggaaagc atcaccgtgc
    15841 tgcctgccga tgacaaaggt ccggtttccg ttacgaagat catcgacggc gcagagctgc
    15901 tgatcccgat ggctggcctc atcaacaaag aagatgagct ggcgcgtctg gcgaaagaag
    15961 tggcgaagat cgaaggcgaa atcagccgta tcgagaacaa gctggcgaat gaaggctttg
    16021 tcgcccgcgc accggaagcg gtcatcgcga aagagcgtga gaagctggaa ggctatgcgg
    16081 aagcgaaagc gaaactgatt gaacagcagg ctgttatcgc cgcgctgtaa tcaaaacaca
    16141 gtgatgaaaa cgaaggccgg agcatgctcc ggcctttttt atctcttaca gaaatggtaa
    16201 agaaggcata attccccgtg aaatactggc taagaatcct ttatctggtt gttcttcttt
    16261 attgattaaa ggaaggtcat ccaaatcgcc cagcaaatac gtattttttg cctgccagtc
    16321 aatcatccat attttcagca gtggataagc cagaccgccc gtaatcccgg atatcaccac
    16381 caacgcacac atgcgataaa gcataccgtg gtatgttaaa gttgagcgaa aacgaatacg
    16441 cccatcattc agtgacaggt tgctcataaa atgatttcgc aacgacaccg ttaaataact
    16501 ggtgctaaca gcaatcccaa aataatagat taactgcgcg attatcattt tacgttgcat
    16561 ttccataaat tgctgtaaat tctcaatatc atcatttgca tacccagaac tatcatactc
    16621 atttaatatt tgatcgaaga taatataacc agcaacagcg agaaaaggta ataaagccaa
    16681 aaatgcgaga atggcatatt ttatacaata tgtagtatct aatttcacct tgaaacgatg
    16741 tataccgaaa ctggtattac tccagagaaa actcattacc agactatata aagtgccatt
    16801 aaaaatacca atgaaaacaa tacaaactat tgtcatcaga accacagata taataacgct
    16861 acttgaacta ttggcatgta gcatctttgt cgagataaag aaaacagtcc ccatcccaag
    16921 ggccattaaa atcggcaaga aaaaggtcac ccaccagaac cctttcatag agcaattaaa
    16981 actaaatctt acgccgttga gactggtcat caaggcctga taacgcagtc cttttgctgc
    17041 cataaatata agcaaaacca acagtgacaa agtcaaaaca cagccaataa gcggcatatc
    17101 tgctgacact gtcattaaga ttgcgaaata gaaaaaaaca aaaacaagac aactaacaaa
    17161 aacattccca ccggtaatcc cataagaaaa tcgttgtccg ttaacttcca tattagcata
    17221 gagataacgc ttacatttca ttaatgccca tggtaaatag atacctagcg ttataatcgt
    17281 taacaacaca ttcaccacac aaataagaaa atatctctca cctgtaccat gaaaaacaaa
    17341 cgaatgggac ggaacatcca tatcattaat aacttgagcc ataaaagttc cctgaattaa
    17401 aaaatccatt gatataattt atatatacag acagtaaaat gttctgcc
//
LOCUS       DADMKF010000078        17396 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.079, whole genome shotgun sequence.
ACCESSION   DADMKF010000078 DADMKF010000000
VERSION     DADMKF010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17396
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.079"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(<1..161)
                     /locus_tag="JNC24_002424"
     CDS             complement(<1..161)
                     /locus_tag="JNC24_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAY9159152.1"
                     /translation="MNNNNTLYVGLDVHKESITVAYAINSEPVELMGKIGTSPTDIQN
                     LCKRLRSKSS"
     gene            288..482
                     /locus_tag="JNC24_002425"
                     /pseudo
     CDS             288..482
                     /locus_tag="JNC24_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            705..1202
                     /gene="ubiC"
                     /locus_tag="JNC24_002426"
     CDS             705..1202
                     /gene="ubiC"
                     /locus_tag="JNC24_002426"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HAY9159153.1"
                     /translation="MSHPALTQLRALRYFKEIPALDSQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            1215..2087
                     /gene="ubiA"
                     /locus_tag="JNC24_002427"
     CDS             1215..2087
                     /gene="ubiA"
                     /locus_tag="JNC24_002427"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HAY9159154.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSILVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(2242..4665)
                     /gene="plsB"
                     /locus_tag="JNC24_002428"
     CDS             complement(2242..4665)
                     /gene="plsB"
                     /locus_tag="JNC24_002428"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HAY9159155.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RLYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRPLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            4836..5204
                     /locus_tag="JNC24_002429"
     CDS             4836..5204
                     /locus_tag="JNC24_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAY9159156.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            5314..5922
                     /gene="lexA"
                     /locus_tag="JNC24_002430"
     CDS             5314..5922
                     /gene="lexA"
                     /locus_tag="JNC24_002430"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repressor LexA"
                     /protein_id="HAY9159157.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            5941..7320
                     /gene="dinF"
                     /locus_tag="JNC24_002431"
     CDS             5941..7320
                     /gene="dinF"
                     /locus_tag="JNC24_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709876.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HAY9159158.1"
                     /translation="MPPGVAVCFSSLFIRLVCMAFLTSSDKALWHLALPMIFSNITVP
                     LLGLVDTAVIGHLDSPVYLGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQA
                     LARALVQPLLLALGAGALIALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPAS
                     LANLVLLGWLLGVQYARAPVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYA
                     TLLIGLLMVRKILKLRGISGEMSKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVL
                     GARLGSDIIAVNAVLMTLLTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACR
                     QSGIVALLFSVVYLLAGEHIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMF
                     IGATRAAEMRNSMAVAAAGFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHW
                     RNGTWFAAT"
     gene            7436..7645
                     /locus_tag="JNC24_002432"
     CDS             7436..7645
                     /locus_tag="JNC24_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAY9159159.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(7687..8202)
                     /gene="zur"
                     /locus_tag="JNC24_002433"
     CDS             complement(7687..8202)
                     /gene="zur"
                     /locus_tag="JNC24_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HAY9159160.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
     gene            8520..8774
                     /locus_tag="JNC24_002434"
     CDS             8520..8774
                     /locus_tag="JNC24_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9159161.1"
                     /translation="MLKIIPGATGYFNKTLNSNQFDNEDAIKDKLDNRGSIKGKLNNI
                     YGKSIDYAALRHRDIIIAKIDLFIQRITHNLWHARKKMCF"
     gene            8798..9505
                     /locus_tag="JNC24_002435"
     CDS             8798..9505
                     /locus_tag="JNC24_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 family protein"
                     /protein_id="HAY9159162.1"
                     /translation="MWVNKYIDDCTDEDLNDRDFIASVVDRAIFHFAINSICNPGDNK
                     DATPIEQCTFDVETKNGLPSTVQLFYEESKDNEPLANIHFQAIGSGFLTFVNACQEHD
                     DNSLKLFASLLISLSYSSAYADLSETVYINENNESYLKAQFEKLSQRDMKKYLGEMKR
                     LADGGEMNFDGYLDKMSHLVNEGTLDPDILSKMRDAAPQLISFAKSFDPTSKEEIKIL
                     TDTSKLIYDLFGVKSEK"
     gene            9868..10905
                     /gene="dusA"
                     /locus_tag="JNC24_002436"
     CDS             9868..10905
                     /gene="dusA"
                     /locus_tag="JNC24_002436"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HAY9159163.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIERELSQGTYLGHITRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            11039..11281
                     /gene="pspG"
                     /locus_tag="JNC24_002437"
     CDS             11039..11281
                     /gene="pspG"
                     /locus_tag="JNC24_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HAY9159164.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKVPKYQRYDRWRY"
     gene            complement(11447..12430)
                     /locus_tag="JNC24_002438"
     CDS             complement(11447..12430)
                     /locus_tag="JNC24_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HAY9159165.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDRVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREEDLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            12513..13928
                     /locus_tag="JNC24_002439"
     CDS             12513..13928
                     /locus_tag="JNC24_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase DnaB"
                     /protein_id="HAY9159166.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            13981..15060
                     /gene="alr"
                     /locus_tag="JNC24_002440"
     CDS             13981..15060
                     /gene="alr"
                     /locus_tag="JNC24_002440"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HAY9159167.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSIGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            15313..16506
                     /gene="tyrB"
                     /locus_tag="JNC24_002441"
     CDS             15313..16506
                     /gene="tyrB"
                     /locus_tag="JNC24_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HAY9159168.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEATNSVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     SAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
     gene            complement(16729..17271)
                     /locus_tag="JNC24_002442"
     CDS             complement(16729..17271)
                     /locus_tag="JNC24_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001371118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9159169.1"
                     /translation="MVTNFITPDGDDDMNISYVNSNKTTSLPVELDALNNKDISYAKD
                     FSYAKDFFLYIETQLKIAKDFCRPGEEVSSSIASKVFHAFIDLVNKIRGKKDFMYICT
                     LCCFAEEVKGDYSHYRTFLFDIGNQYKVKLTQSGKKEFSLTLEFNDTIIESQKVTGNK
                     AKYILEDIEIFYRNKPDTYY"
BASE COUNT         4210 a         4094 c         4504 g         4588 t
ORIGIN      
        1 gatgactttg acctgagacg tttacaaaga ttctgaatat cagtaggtga tgtgccaatt
       61 ttacccatca gttcaacagg ttctgaattg atagcataag cgacagtaat cgattctttg
      121 tgaacatcta acccgacgta caatgtgtta ttgttgttca tgctggcctc cgtggtggaa
      181 ataccggggt gtagcaacag ctcacccatg cggctctgac aacgatgtta acccgcgtta
      241 aatatcccac gttggccagc atctgtctca cggaaagtca tactgcctta catgatatgt
      301 tgaatcctac aaaagatacc aattggaatt ccacgtgtat ctataaatcc agacataaga
      361 tgttgcctat taatcttact caggaaacac tttttagctc caaatctcat ggtaaatatg
      421 cgcttttccc aatttttact gcgagttggc gagctactcg tataaagaat ataggtattt
      481 aagtaaagga aaacatcacc gttcctggca tcatgggcgg tgatgcccct acggttgccc
      541 tcgccagcac gggcatcggc aaagcgtaag gttcaacagc gttttaccac ttcatgcgat
      601 tgttgcgttt ttgttgcgca ttagatcact taatttgctt tacatctccc gtaaacactt
      661 ttctgcgata caatgccttt acgttatgta acggagagtt cggcatgtca caccccgcgt
      721 taacgcaact gcgtgcgctg cgctatttta aagagatccc tgccctggat tcgcaactgc
      781 tcgactggct gttgctggaa gattccatga caaaacgttt tgaacagcag ggaaaaacgg
      841 tgagcgtgac gatgatccgc gaagggtttg tcgagcagaa tgaaatcccc gaagaactgc
      901 cgctgctgcc gaaagagtct cgttactggt tacgtgaaat tttgttatgt gccgatggtg
      961 aaccgtggct tgccggtcgt accgtcgttc ctgtgtcaac gttaagcggg ccggagctgg
     1021 cgttacaaaa attgggtaaa acgccgctag gacgctatct gttcacatca tcgacattaa
     1081 cccgggactt tattgagata ggccgtgatg ccggactgtg ggggcgacgt tcccgcctgc
     1141 gattaagcgg taaaccgctg ttgctaacag aactgttttt accggcgtca ccgttgtact
     1201 aagaggaaaa aaatatggag tggagtctga cgcagaataa gctgctggcg tttcatcgct
     1261 taatgcgtac ggataagcca attggcgcgt tactgctgct ctggccaaca ttatgggcgt
     1321 tgtgggtggc gacaccgggc gttccccagc tctggatcct ggcggtgttt gtcgcgggtg
     1381 tctggctgat gcgcgctgcc ggatgtgtgg tgaatgatta tgctgaccgc aagtttgatg
     1441 gtcatgttaa gcgcacggcg aaccgaccgc ttcccagcgg cgcggtaacg gagaaagagg
     1501 cgcgcgcgct gtttgtagtg ctggtattga tttcattttt actggtgctg acgctgaata
     1561 cgatgaccat tctgttgtcg attgccgctc tggcgttggc gtgggtgtac ccgtttatga
     1621 agcggtatac ccatctcccg caagtggtgc tgggcgcggc gtttggctgg tcgattccaa
     1681 tggcttttgc cgctgtgagt gagtcggtgc cattgagttg ctggttaatg ttcctcgcca
     1741 atattctctg ggcggtggct tacgacacgc agtatgcaat ggttgaccgt gacgatgatg
     1801 tgaagattgg tattaaatcc accgcaatcc tgttcggcca atacgataaa ttgattattg
     1861 gtattttgca gattggcgta ctggcactga tggcgatcat cggtgagtta aatggcttag
     1921 gctggggata ttactggtca attctggtgg ctggcgcgct gtttgtttat caacaaaaac
     1981 tgattgccaa ccgcgagcgt gaagcctgct ttaaagcatt tatgaataat aactatgttg
     2041 gtctggtact gtttttagga ctggcaatga gttactggca tttctgatga tgtaaaaaag
     2101 ccggatgatc atccggcttt cttctgggtt gcctgatgcg ctgcgcttct caggcctaca
     2161 caacacatcg caatttattg aatttgcaga ttatggaagg ccggataagg cgttttcgcc
     2221 gcatccggca attctctctg attacccttc gccctgcgtc gcactctcaa tcgtcaaacg
     2281 cacgtctgat gtaatcaact ccgccagcaa ctgataaacc ttcatcgttt ctgccggttc
     2341 ggcgtcgccg ctatcgctga tatacccttc atcacgcagc gtcagcacca gagaactgaa
     2401 caccgccttg tcgaagaact ccggcgcgtt gatgccgtgc agcacggaga gacgttgcgc
     2461 aacggtgcgg ctctctttct ccagcgtacc gcggttgatc gacgggttgg cactcaacaa
     2521 ccagaaggtg atggcataac gttgcagcgt ttcgcgcgcg cctgcggcca gcagctgtag
     2581 ggggcgagaa tgcgccgggt tgatatgcaa ctcatcatct tgcagggtaa tcagcccctg
     2641 acgttgcatc tcatttgcca gcgcatcaat aacgtccggc aactcgtcgc gatcccagcg
     2701 caggaacagc tccgctttca gcattgggta aagcacattg acgtgctcca tcaatacgtc
     2761 gcgggagatg tggcgatgct gggtgacaat tgccgccatc agcgaaggca gcaccaacat
     2821 atgcgcaatg ttgttgcgat agtaggtcat cagcaccgct tgctcgcgcg gcagaatgat
     2881 gatgtcgccg atagtgtctt tctcgacttc aaacttgttc atttgcagcg cgtgatcgat
     2941 aagctcgctg gcgctggctg aaggaacggt agagtccgtg gagtaaggta cgttgcgcat
     3001 cagatccagg tagcagttga gttgctcggt taactgctcg cgggtgagtg agcgctgacg
     3061 tgatgccaga agcgcagtac aacacaggtt catggcgttt gccgcgcctg cgttgttaat
     3121 gcgtaccatc agatcggcag caatattatt gaccgtcggc gttaaccagg ccggacgcac
     3181 cgcttcgatg ggatcgatag attcacgcca gtctggtacg tgctggttaa ggtaggtcat
     3241 caacggcatt ggttcaccga agttgacgta accctgaccg agattacgca gcttgcttaa
     3301 accgcgcagc atctgcggca ggctctcttt ctctttcgtc gcgccgcgca gttctttggc
     3361 gtaagtaccc acttccatga cgtgctcata accgatatag atcggaatca gcgtaatcgg
     3421 acgcgtgccg ccacgcagca tcgcctgaat ggtcatcgac agcgtaccgg ttttcggatc
     3481 cagcaaacgc cccgtgcggg aacgaccgcc ttccacgaag tactcgacgg aataaccacg
     3541 gctgaacagt tcgccgagat actcacggaa aacggtggaa taaagtttat tgcctttaaa
     3601 agtacggcga ataaagaacg cccccagacg gcggaaaatc ggcccggccg gccagaagtt
     3661 caggttgatc ccggcggcga tatgcggcgg caccagcccc tggtggtaca gcacgtaaga
     3721 aagtagcagg tagtccatgt gactgcggtg gcaaggcaca tacaccagct catgaccgtc
     3781 gtgggccagc tggcgaacgc gctcggcgtt atggacgttg atgccctgat aaagtcggtt
     3841 ccaggtgaag cccagaatgc ggtcagtcag gcggatcatc tcgtaagaga aattcgccgc
     3901 aatctcttcc atcagcgcaa tcgcgttctg ctgcgctttt tcatgggaga tttttttgct
     3961 gcgcgcttca tcttctaccg ctttggcaat ggcgcgggag gcgagcagct tattaaacag
     4021 atcctgacga gcaggcagac gtgggcctac ggcagccaga cgttgacggg caaagtgcat
     4081 acgcgccacg cgcgccagtt tctgtgcgat agttttatcc gtgccgtgtt catccgccat
     4141 acggcgcagc gaaactgacg gcgagaaacg cacaaaactg tcgcgaccga gccacagtac
     4201 ggcgaaaaat ttctgtacgc cgttaagcat acgcagcggc gggtttactt cgcctttttc
     4261 acgccccggc gcgcgaccaa acatcaccga tactggcacc atctgcacat ccagatttgg
     4321 gttgctacgg tgcaaatcga gatagtcgtg gaacagctta atagactctt ctttcggcgt
     4381 gtaataggtg aatacacgcg gcccgccgtg aatgaacaca tagcgcggca gtagcgtgcc
     4441 gtcgatttct agcggctcta acgggtcagg caagtcatga gccagacact gggcgcgtaa
     4501 cgtcagcaaa tctgctttcg agttgtacgg taaaacgtac ataattggac gagaggtatc
     4561 cagccccagt tccggggcag gatctgccgg aatagacttg ctttttacca ggatgcttaa
     4621 tggtaaattc agtaatttgt agtaaattcg tggccagccg gacataaacg atgtaaagcc
     4681 tctggttaat aatgcaaatg cgcggcaagg atagcagaaa gtcatgggaa attctgtggt
     4741 atccgctcat gtttcgcgcg gcgttacgca aacccgaatc atcggattta acggtacact
     4801 gatattgacg ctcataatgt aaaaaggttc tttcaatggc caataatacc actggattca
     4861 cccgaattat caaagctgct ggctattcct ggaaaggttt acgcgctgca tggatcaacg
     4921 aagcggcatt ccgtcaggaa ggcgtagcgg tattgttggc ggtggtcatc gcctgctggc
     4981 tggatgtgga cgcaattacc cgcgtgctgc ttatcagctc cgtgatgctg gtgatgattg
     5041 tggaaattct caatagcgcc atcgaagcag tggttgaccg aattggctct gaataccatg
     5101 agctttccgg acgcgctaaa gatatgggat ccgccgcggt gctgattgcg attatcgtcg
     5161 ccgtgataac ctggtgcatc ctgttatggt cgcattttgg ataacccttc cagatttcga
     5221 taaatctctg gtttattgtg cagtttatgg ttccaaaatc gccttttgct gtatatactc
     5281 acagcataac tgtatataca cccagggggc ggaatgaaag cgttaacggc caggcaacaa
     5341 gaggtgtttg atctcatccg tgatcacatc agccagacag gtatgccgcc gacgcgtgcg
     5401 gaaatcgcgc agcgtttggg gttccgttcc ccaaacgcgg ctgaagaaca tctgaaggcg
     5461 ctggcacgca aaggcgttat tgaaattgtt tccggcgcat cacgcgggat tcgtctgttg
     5521 caggaagagg aagaagggtt gccgctggta ggtcgtgtgg ctgccggtga accgcttctg
     5581 gcgcagcagc atattgaagg tcattatcag gtcgatcctt ccttgttcaa gccgaatgct
     5641 gatttcctgc tgcgcgtcag cgggatgtcg atgaaagata tcggcattat ggatggtgac
     5701 ttgctggcag tgcataaaac tcaggatgta cgtaacggtc aggtcgttgt cgcacgtatt
     5761 gatgacgaag ttaccgttaa gcgcctgaaa aaacagggta ataaagtcga actgttgcca
     5821 gaaaatagcg agtttaaacc aattgtcgtt gaccttcgtc agcagagctt caccattgaa
     5881 gggctggcgg ttggggttat tcgcaacggc gactggctgt aacatatctc tgagaccgcg
     5941 atgccgccag gcgtcgcggt ttgtttttca tctctcttca tcaggcttgt ctgcatggca
     6001 ttcctcactt catctgataa agcactctgg catctcgcct tacccatgat tttctccaat
     6061 atcaccgttc cgttgctggg actggtcgat acggcggtaa ttggtcatct tgatagcccg
     6121 gtttatttgg gcggcgtggc ggttggtgca acggcgacca gctttctctt tatgctgttg
     6181 ctgtttttac gcatgagcac caccgggctg actgcgcagg cttatggtgc caaaaatcct
     6241 caggcattag cccgtgcgct ggtgcaaccg ttgctgttgg cgttgggggc tggggcgtta
     6301 attgcgctgc tgcgtacacc gattatcgat ctggctctgc atattgttgg cggtagtgaa
     6361 gcagtcctgg aacaggcgcg gcgctttctt gaaatccgct ggttaagcgc accggcgtcg
     6421 ctggcgaatc tggtattact cggttggtta ctcggcgtgc aatatgcccg tgcgccagta
     6481 attttgttag tggtcggcaa tatcctcaac attgtgctgg atgtctggct agtgatgggg
     6541 ctgcatatga acgtgcaggg cgcggctctg gcgacggtta ttgcggaata tgcaacattg
     6601 ctgattggtc tgctaatggt gcgtaaaatc ctcaaactac gcggaatttc cggcgaaatg
     6661 tcgaaaactg cctggcgagg aaacttccgt cgcttgctgg cgcttaaccg cgatatcatg
     6721 ctgcgttcgc tgttgttgca actctgtttc ggcgcgatca ccgtacttgg cgcgcgactg
     6781 gggagtgaca ttatcgctgt aaacgcggtt ctgatgacgc tactcacctt taccgcctat
     6841 gcgctggatg gttttgccta cgcggttgaa gcacattccg gtcaggcata cggtgcgcgc
     6901 gacggtagcc agttgctgga tgtctggcgg gcagcgtgcc gccagtcggg gatcgtagcg
     6961 ttactatttt cggtggttta tttgctggca ggggaacaca tcattgcgtt gctgacgtcg
     7021 ttaacccaga ttcagcagct ggctgaccgc tatcttatct ggcaggtgat tttgccgttg
     7081 gttggcgtct ggtgttatct gctggacggc atgtttatag gcgcaacgcg tgccgccgaa
     7141 atgcgtaaca gtatggcggt ggccgccgca ggttttgcgc tgacgctcct tacgctgccg
     7201 tggctgggta atcatggttt gtggctggca ctaaccgtct ttctggcgtt gcgcgggctt
     7261 tctctggcgg ctatctggcg gcgtcactgg cgtaacggta cctggtttgc cgcaacgtga
     7321 cggttaaaaa ttctgaataa attatcctaa gccaaattgc tgactacact taatctcacg
     7381 ttcagaagaa aagtgaacgt actctcattc acaacctaac gatgaggtct tgattatgaa
     7441 taaagatgaa gccggcggta actggaaaca gtttaaaggt aaagtgaaag agcaatgggg
     7501 caaactgacc gatgatgata tgacgatcat tgaaggtaaa cgtgatcaac tggtcggtaa
     7561 aatccaggaa cgttatggtt atcagaaaga tcaggcagaa aaagaggtcg tggattggga
     7621 aacccgcaat gaatatcgct ggtaattaat ccctcctgcc cgacgtgtac aaggatgtac
     7681 gccctcttaa cgcggtttct ttttcacctg cacagagtga tcatgctggc actgttcagg
     7741 atgacgacac gcttccactt ccacgcatgc cgcacagagc ccatgtgctt caatcacatt
     7801 atgccgcagg gcaaacccca tttttgccgc cagcgtatgc ataatgtctt ccacgccttc
     7861 tgcacactct tctttcaccg cgccgcagcg atcgcatata aacatggctg acgtatgcgt
     7921 gggctggtcg aacagatgac agagcacata actgttggtg gattccacct tatgcacaaa
     7981 accttgctca agcagaaaat ccagcgcgcg ataaaccgtt ggcggcttgg cttgcggttc
     8041 agcttcgcgc agtaaatcaa gcagatcata agcgctgata gcgccatcct gcagactcat
     8101 caggcgcaac acttccaggc gctgtggggt caggcgcaca ttacgctgcg cgcagatttt
     8161 ttcagcctgc gctaataact cctgcgttgt ggtcttttcc atcgggcacc tcaaagtggg
     8221 ggttaagaat gtccttactt taccatgttc caggaaaaac accgagaacc cttattgttg
     8281 ccgtaatgtt gattttctgt tttgtaggta aggtgttatg ttgccttgtc gtaccattat
     8341 caacacgata ataattaata attcattttt aaataaattg cttcaagtta actcgctatt
     8401 tatttaactg ggtttaaacg catacctgta gttcagaaat tatgagcgat ttttgatagt
     8461 gaccagatgg caaaagaatt ataaggagga cacggtacga caaaaaaggt agatgaatca
     8521 tgttgaagat aatacccggt gcaactggat actttaataa aacactcaat tcaaatcaat
     8581 tcgataatga agatgcaatc aaagataaat tagataatag aggttcaatc aaaggtaaat
     8641 taaataatat atatggtaaa tctattgatt atgcagcgct gcgtcatcgg gatataatta
     8701 ttgcaaaaat agatttgttt attcaacgaa tcacgcataa tttatggcat gcacgtaaaa
     8761 aaatgtgttt ttaattgagc aaataaatga tctcaaaatg tgggtcaata agtatattga
     8821 cgattgcact gatgaggatt taaacgatcg tgactttatt gcatcagttg ttgaccgggc
     8881 tatttttcat ttcgcgatta atagtatatg taatcctggg gataataaag atgcgactcc
     8941 cattgaacaa tgtacttttg atgtagaaac taagaatggc cttccctcca cggttcagct
     9001 attttatgag gaatctaagg ataatgaacc tttagcgaat atacattttc aagcaatagg
     9061 ttctggtttt ttaacgtttg ttaatgcctg ccaggaacat gatgacaaca gcttaaaatt
     9121 atttgcttca ctgttaattt cactttcata ttctagtgcc tacgcagatt tatcagaaac
     9181 agtgtatatt aatgaaaata atgagagcta cctgaaagct cagtttgaaa aattatctca
     9241 acgtgatatg aagaagtacc tgggagagat gaagcgtctg gctgatgggg gagaaatgaa
     9301 ttttgatggc tatctggata agatgtcaca tctggtgaat gaaggaacgc tcgatcctga
     9361 tattttaagc aaaatgcgag atgctgcacc acaattaatt agcttcgcga agtcgtttga
     9421 cccaacctca aaggaagaga ttaaaatact tacagacact tctaaattaa tttatgattt
     9481 gttcggggtt aaatcggaga aataatatgt gaagttcttc gatagtatgg aaggcattat
     9541 ataaaaggac ccaatattta ttgggttctt ttttctctat caatgctatt agcagggaga
     9601 tatatcacca gagtttaatg tgtgattttt tatttatcgt cgaacctgga ttgtttatca
     9661 ttggccttaa caaagttaac ggctaataag attatttcca tcacttcgtg agagcttcat
     9721 gccttgagag gatctcaatt ttcttttgca atgagacagg cgcttcctgt tgttatggta
     9781 tagtaccccg ctattgagcc tcctgaatag tgatgctgaa taacataacc ccatgatata
     9841 tcgataaaat aatctctaca tttgaaaatg cacggtaatt ctgaaatgca aaaaatcaac
     9901 caaaccagcg caatgcctga aaaaactgac gttcactgga gtggtcggtt tagcgttgca
     9961 ccaatgctcg actggacgga cagacattgc cgctatttct tgcgtctgct ttcccgcaat
    10021 acgttgctgt ataccgaaat ggtgaccaca ggggcgatta ttcacggtaa aggtgattac
    10081 ctggcgtaca gtgaagaaga acatccggta gcgttgcaac tcggcggtag cgatccggcg
    10141 gcgctggcac agtgtgcgaa gctggcagaa gcgcgtggat atgatgagat caacctgaat
    10201 gtcggctgcc cgtctgaccg ggtgcagaac ggcatgtttg gtgcgtgtct gatgggtaat
    10261 gcgcagctgg ttgccgactg cgtgaaagcg atgcgcgatg tggtgtcgat tccagtgacg
    10321 gtgaaaacgc gtattggcat cgatgaccag gacagctatg aatttctctg cgatttcatc
    10381 aacaccgttt ccggcaaagg cgagtgtgag atgtttatca tccacgcacg taaagcctgg
    10441 ctttcggggt taagcccgaa agaaaaccgt gaaatcccgc cgctcgatta tccgcgtgtg
    10501 tatcaactga agcgtgactt tccgcatctg acaatgtcga ttaacggtgg tatcaagtcg
    10561 ctggaagagg ccaaagcaca cctgcaacat atggatggcg tgatggtcgg gcgcgaggcg
    10621 tatcagaatc cgggtattct ggcggcggta gaccgggaga tctttggttc ctcggatacc
    10681 gatgccgatc cggtggcggt agtgcgcgcc atgtatccgt acattgagcg tgaactcagc
    10741 caggggacgt atctcggcca tattacccgg catatgttgg gcttgttcca gggtattcct
    10801 ggcgcgcggc agtggcggcg ttatttaagt gaaaatgccc ataaagcggg tgcagacatt
    10861 aatgtgctgg aacacgcgct caaactggtg gcggataagc gttaactttt caccaaaaag
    10921 tagtcaaatt caccacgccc tgcgcaccgt cgcggggcgt tttgctgtta aatcaataga
    10981 ttatttttgg catgattctt gtaatgccag caagagattt catatttggg agagcatcat
    11041 gctggaacta ctttttgtga ttggcttttt tgtcatgctg atggtcaccg gcgtttcgtt
    11101 gctgggcatt atcgccgcgc tggttgtggc gacggccatt atgttcctcg gcggtatgct
    11161 ggcattgatg attaagttgc tgccgtggtt actactggcg attgcggtgg tgtgggttat
    11221 taaggcgatt aaagcaccaa aagtgccgaa atatcagcgt tatgaccgct ggcgttacta
    11281 agggattgtg cggatgatca caacctaagg ttttatcctt agaacaaaat aggaattgat
    11341 aatcaaatct gtcactattg cgcctctaac agattcatcg tgctgtaccc tacatacagc
    11401 cgaactataa aaagaaaggg cttcccaggt ggaagcccta tttcttttat ggaatcaaca
    11461 ggctggaacc ttgcgtcgcc cggctttcca gaatctcatg cgcacgctgc gcatccttca
    11521 gcggatattt ctgctgctcg gcgacatcga ccttaatcac accgctggca atcaaagaga
    11581 acagttcatt actggcctcg gttaattcct cccgcgtggt gatatagcct tgcagggaag
    11641 ggcgtgtcac atacaacgag cctttttgat tgagaatgcc taagttcaca ccggtaaccg
    11701 cacctgatga gttgccaaaa ctgaccatta agccgcggcg ttgcaggcaa tccagcgacc
    11761 gttcccaggt gtctctgccc acggaatcgt acaccacgcg cactttctta ccgccggtga
    11821 tctcttttaa ccgctcgacc agatcctctt cacgatagtt aataacctgc cacgcgcccg
    11881 cttttagcgc gctctgcgct ttttgcgcgg ttcctacggt gccgataagt ttcgcgccca
    11941 gggcttttgc ccactggcag gcaattaagc caacgccgcc agccgctgcg tggaacagga
    12001 actgttcatc gggtttaatt tcataggttt tgcgcagcag ataataaacc gttaagcctt
    12061 tcaggaagga tgccgcagct tgctcaaaag aaattgccgc aggcagaatc gccgctttat
    12121 ccgcattaat gttatgcaca gagctgtaag cgcctaacgc cgactgcgca tagactacac
    12181 gatcgcctgc cttaatatgc tttacaccac tgccgacttt actcacgatg cctgccgctt
    12241 cggtacctaa tccgctgggt agtgatggcg gcgggtaaag gccgctgcgg atatacgtgt
    12301 cgataaaatt gatgccgatg gctttatttt cgacctggat ttcattctct gccggatcgg
    12361 caggagtgaa ctctacggct tgaagtactt ccgggccacc gtgcttgtga aattcaattc
    12421 gtgttgccat gtgtcctcca gaacgtatcg tcagggtctg cttcatatgg taaagtttcg
    12481 acccattctt tatctcggta actccattca ctatggcagg aaataaaccc ttcaacaaac
    12541 agcaggctga accccgcgaa cgcgatccac aagttgccgg gctgaaagtg cctccgcact
    12601 cgatcgaagc ggagcagtcg gtgttgggcg gtttaatgct ggataacgaa cgctgggacg
    12661 atgtagccga gcgtgtggtg gcagacgatt tttacacccg cccacaccgc catatcttta
    12721 ctgaaatggc gcgtttgcag gaaagcggta gtcctatcga tctgattacc cttgcggaat
    12781 cgctggaacg ccaggggcaa ctcgatagcg tcggtggttt tgcttatctg gcagagctgt
    12841 caaaaaatac gccaagtgcg gcgaacatca gtgcttatgc tgacatcgtg cgtgaacgtg
    12901 ccgttgtccg tgagatgatc tcggttgcga atgagattgc tgaagccggt tttgatccgc
    12961 aggggcgtac cagcgaagat ctgctggacc ttgctgaatc ccgcgtcttt aaaattgccg
    13021 aaagtcgtgc aaacaaagac gaagggccga agaacatcgc cgatgtgctc gacgcaaccg
    13081 tggcgcgtat tgagcagttg tttcagcagc cacacgatgg cgttaccgga gtaaacaccg
    13141 gttatgacga tctcaacaaa aaaaccgctg gcttgcagcc gtcggatttg atcatcgtcg
    13201 ccgcgcgtcc gtcgatgggt aaaacaacat ttgcgatgaa cctcgtcgaa aacgcggcga
    13261 tgttgcagga taaaccagta cttatcttct cgctggagat gccttcagaa cagattatga
    13321 tgcgttctct ggcgtcgctg tcgcgcgttg accagactaa aatccgtacc gggcagctcg
    13381 atgatgaaga ctgggcgcgc atttccggca ccatgggtat tttgctcgaa aaacgcaata
    13441 tctatatcga tgactcctcc ggcctgacgc caacggaagt gcgttcccgc gcacgccgta
    13501 ttgcccgtga acacggcggc atcgggctta tcatgatcga ctacctgcaa ctgatgcgcg
    13561 taccggcgct ttccgataac cgtacgctgg aaattgcaga aatctctcgc tcgctgaaag
    13621 cactggcgaa agaactgaac gtgccggtgg tggcgctgtc ccagttgaac cgttctctgg
    13681 aacaacgtgc cgacaaacgc ccggtcaact ccgacctgcg tgaatctggc tctatcgagc
    13741 aggatgcgga cttgattatg tttatctatc gtgatgaggt gtatcacgaa aacagtgatt
    13801 taaaaggcat cgcggaaatt attatcggta aacaacgtaa cggcccaatc gggacggtac
    13861 gcctgacctt taacggtcaa tggtcgcgct tcgacaacta tgcggggccg cagtacgacg
    13921 acgaataata attattttat gaattaggta attaaagcaa acacttatca aggaacacaa
    13981 atgcaagcgg caactgttgt gattaaccgc cgcgctctgc gacacaacct gcaacgtctt
    14041 cgtgaactgg cccctgccag taaaatggtt gcggtggtga aagcgaacgc ttatggtcac
    14101 ggtcttcttg agaccgcgcg aacgctcccc gatgctgacg cctttggcgt agcccgtctc
    14161 gaagaagctc tgcgactgcg tgcgggggga atcaccaaac ctgtactgtt actcgaaggc
    14221 ttttttgatg ccagagatct gccgacgatt tctgcgcaac attttcatac cgccgtgcat
    14281 aacgaagaac agctggctgc gctggaagag gctagcctgg acgagccggt taccgtctgg
    14341 atgaaactcg ataccggtat gcaccgtctg ggcgtaaggc cggaacaggc tgaggcgttt
    14401 tatcatcgcc tgacacagtg caaaaacgtt cgtcagccgg tgaatatcgt cagccatttt
    14461 gcgcgcgcgg atgaaccaaa atgtggcgca accgagaaac aactcgctat ctttaatacc
    14521 ttttgcgaag gcaaacctgg tcaacgttcc attgccgcgt cgggtggcat tctgctgtgg
    14581 ccacaatcgc attttgactg ggtgcgcccg ggcatcattc tttatggcgt ctcgccgctg
    14641 gaagatcgct ccatcggtgc cgattttggc tgtcagccag tgatgtcact aacctccagc
    14701 ctgattgccg tgcgtgagca taaagccgga gagcctgttg gttatggtgg aacctgggta
    14761 agcgaacgtg atacccgtct tggcgtagtc gcgatgggct atggcgatgg ttatccgcgc
    14821 gccgctccat ccggtacgcc agtgctggtg aacggtcgcg aagtaccgat tgtcggacgc
    14881 gtggcgatgg atatgatctg cgtagactta ggtccacagg cgcaggacaa agccggggat
    14941 ccggtcattt tatggggcga aggtttgccc gtagaacgta tcgctgaaat gacaaaagtg
    15001 agcgcttacg aacttattac gcgcctgact tcaagggtcg cgatgaaata cgtggattaa
    15061 tcgttctgta atatttgatt gtctgtgccg gatgcggcgt gaatgcctta tccggccaat
    15121 aaaatcctaa aaattcaata agttgatgtt ctttcatgct cttataaagg tcgtgcctct
    15181 ggcggatgtt cgtttgtcat gagtctcact ctgttgctaa ttgccgttcg ctcctgaaca
    15241 tccactcgat cttcgccttc ttccggttta ttgtgtttta accacctgcc cgtaaacctg
    15301 gagaaccatc gcgtgtttca aaaagttgac gcctacgctg gcgacccgat tcttacgctt
    15361 atggagcgtt ttaaagaaga ccctcgcagc gacaaagtga atttaagtat cggtctgtac
    15421 tacaacgaag acggaattat tccacaactg aaagccgtgg cggaggcgga agcgcgcctg
    15481 aatgcgcagc cccatggcgc ttcgctttat ttaccgatgg aagggcttaa cagctatcgc
    15541 catgccattg cgccgctgct gtttggtgcg gaccatccgg tactgcaaca acagcgcgta
    15601 gcaaccattc aaacccttgg cggctcaggg gcattgaaag tgggcgcgga tttcctgaaa
    15661 cgctacttcc cggaatcagg cgtctgggtc agcgatccta cctgggaaaa ccacgtagca
    15721 atattcgccg gggctggatt cgaagtgagt acttacccct ggtatgacga agcgactaac
    15781 agcgtgcgct ttaatgacct gttggcgacg ctgaaaacat tacctgcccg cagtattgtg
    15841 ttgctgcatc catgttgcca caacccaacg ggtgccgatc tcactaatga tcagtgggat
    15901 gcggtgattg aaattctcaa agcccgcgag cttatcccat tcctcgatat tgcctatcaa
    15961 ggatttagtg ccggtatgga agaggatgcc tacgctattc gcgccattgc cagcgctgga
    16021 ttacccgctc tggtgagcaa ttcgttctcg aaaattttct ccctttacgg cgagcgcgtc
    16081 ggcggacttt ctgttctgtg tgaagatgcc gaagccgctg gccgcgtact ggggcaattg
    16141 aaagcaacag ttcgccgcaa ctactccagc ccgccgaatt ttggtgcgca ggtggtggct
    16201 gcagtgctga atgacgaggc attgaaagcc agctggctgg cggaagtaga agagatgcgt
    16261 actcgcattc tggcaatgcg tcaggaattg gtgaaggtat taagcacaga gatgccagaa
    16321 cgcaatttcg attatctgct taatcagcgc ggcatgttca gttataccgg tttaagtgcc
    16381 gctcaggttg accgactacg tgaagaattt ggtgtctatc tcatcgccag cggtcgcatg
    16441 tgtgtcgccg ggttaaatac ggcaaatgta caacgtgtgg caaaggcgtt tgctgcggtg
    16501 atgtaatgca ggaaagcagg ctggagttac ccagcctgca gtgaaattaa actgtcgtcg
    16561 ctttcactat ttctttgtag atgatttttt tgatgccgtt gttctacgtg agagataata
    16621 aaacgttgtc agttctttta ttgttaagtt tatcccaatt atctggaatt cctttcccta
    16681 ttttttcgtg tggagtaata atttctgcac atctaaccat tattaaattt aataataagt
    16741 atcgggtttg ttacgataga atatttctat atcctcaaga atatactttg ccttattgcc
    16801 tgtgactttc tgagattcaa ttatagtatc gttaaattct aatgttaaag agaactcttt
    16861 ttttccgctt tgtgtaagtt taaccttata ttgattacca atatcaaata aaaaggtcct
    16921 gtaatgagaa taatcacctt taacctcctc ggcaaaacag caaagcgtgc aaatatacat
    16981 aaagtctttc ttacccctga ttttgttaac taaatcgata aatgcgtgaa aaactttact
    17041 tgcaatagaa cttgatactt cttctccagg tctacaaaaa tcttttgcaa tttttaactg
    17101 tgtttctatg tataaaaaga aatctttagc ataagaaaaa tctttggcat aagagatgtc
    17161 tttattattt agtgcatcta gctcaacagg taaagatgtc gttttgttgc tattcacata
    17221 tgatatattc atatcatcat ctccatcggg agtaataaaa ttagtcacca ttgtaggata
    17281 gggttctggt caatcaggaa tcatctttat aaacttcgat tgtttttgta atgctgtatc
    17341 attaagttca ttaaatcgta cagcagataa atgttctttc aaatttcgct catttg
//
LOCUS       DADMKF010000079        17368 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.080, whole genome shotgun sequence.
ACCESSION   DADMKF010000079 DADMKF010000000
VERSION     DADMKF010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17368
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.080"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            24..1001
                     /locus_tag="JNC24_002443"
     CDS             24..1001
                     /locus_tag="JNC24_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HAY9159170.1"
                     /translation="MVFTSSCCDNLSIDEIIERAEKGDCEAQYIVGFYYNRDSAIDSP
                     DDEKAFYWLKLAAEQGHCEAQYSLGQKYTEDKSRHKDNEQAIFWLKKAALQGHTFASN
                     ALGWTLDRGEAPNYKEAVVWYQIAAESGMSYAQNNLGWMYRNGNGVAKDYALAFFWYK
                     QAALQGHSDAQNNLADLYEDGKGVAQNKTLAAFWYLKSAQQGNRHAQFQIAWDYNAGE
                     GVDQDYKQAMYWYLKAAAQESVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECNDA
                     TAWYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIRETEDLL"
     gene            complement(1071..1553)
                     /locus_tag="JNC24_002444"
     CDS             complement(1071..1553)
                     /locus_tag="JNC24_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HAY9159171.1"
                     /translation="MNKVAQYYRELVASLSERLRNGERDIDALVEQARERVIKTGELT
                     RTEVDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGHDQFQRRPFEP
                     "
     gene            1788..4370
                     /gene="leuS"
                     /locus_tag="JNC24_002445"
     CDS             1788..4370
                     /gene="leuS"
                     /locus_tag="JNC24_002445"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HAY9159172.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNMLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVLVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            4385..4966
                     /gene="lptE"
                     /locus_tag="JNC24_002446"
     CDS             4385..4966
                     /gene="lptE"
                     /locus_tag="JNC24_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HAY9159173.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTTLGN"
     gene            4966..5997
                     /gene="holA"
                     /locus_tag="JNC24_002447"
     CDS             4966..5997
                     /gene="holA"
                     /locus_tag="JNC24_002447"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HAY9159174.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVTARAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQTQLRQAVQLLTRTELTLKQDYGQSVWAELKGLSLLLCHK
                     PLADVFIDG"
     gene            5999..6640
                     /gene="nadD"
                     /locus_tag="JNC24_002448"
     CDS             5999..6640
                     /gene="nadD"
                     /locus_tag="JNC24_002448"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HAY9159175.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            6664..7275
                     /locus_tag="JNC24_002449"
                     /pseudo
     CDS             6664..7275
                     /locus_tag="JNC24_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415171.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
     gene            7535..7852
                     /gene="rsfS"
                     /locus_tag="JNC24_002450"
                     /gene_synonym="rsfA"
     CDS             7535..7852
                     /gene="rsfS"
                     /locus_tag="JNC24_002450"
                     /gene_synonym="rsfA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HAY9159176.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            7856..8323
                     /gene="rlmH"
                     /locus_tag="JNC24_002451"
     CDS             7856..8323
                     /gene="rlmH"
                     /locus_tag="JNC24_002451"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010618862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HAY9159177.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            8354..10255
                     /gene="mrdA"
                     /locus_tag="JNC24_002452"
     CDS             8354..10255
                     /gene="mrdA"
                     /locus_tag="JNC24_002452"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HAY9159178.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNAPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTAHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            10258..11370
                     /gene="mrdB"
                     /locus_tag="JNC24_002453"
                     /gene_synonym="rodA"
     CDS             10258..11370
                     /gene="mrdB"
                     /locus_tag="JNC24_002453"
                     /gene_synonym="rodA"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HAY9159179.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            11381..12469
                     /gene="rlpA"
                     /locus_tag="JNC24_002454"
     CDS             11381..12469
                     /gene="rlpA"
                     /locus_tag="JNC24_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HAY9159180.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTQLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDSVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTAPSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            12608..13819
                     /gene="dacA"
                     /locus_tag="JNC24_002455"
     CDS             12608..13819
                     /gene="dacA"
                     /locus_tag="JNC24_002455"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HAY9159181.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFGDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            13929..14192
                     /locus_tag="JNC24_002456"
     CDS             13929..14192
                     /locus_tag="JNC24_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HAY9159182.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            14293..14934
                     /gene="lipB"
                     /locus_tag="JNC24_002457"
     CDS             14293..14934
                     /gene="lipB"
                     /locus_tag="JNC24_002457"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HAY9159183.1"
                     /translation="MYQDKILVRQLGLQPYEPISQAMHEFTDTRDDSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            15194..16147
                     /locus_tag="JNC24_002458"
     CDS             15194..16147
                     /locus_tag="JNC24_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HAY9159184.1"
                     /translation="MDSNNQIEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHK
                     GIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICREGNPLLSLEDDRETIDNAAHVLLL
                     PEGQNFSGLRQRVQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNEEQIDFSIHYNKFSLRDPILHGVIDVIRNAF"
     gene            16356..17321
                     /gene="lipA"
                     /locus_tag="JNC24_002459"
     CDS             16356..17321
                     /gene="lipA"
                     /locus_tag="JNC24_002459"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HAY9159185.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
BASE COUNT         4335 a         4389 c         4458 g         4186 t
ORIGIN      
        1 tctacaacca taaggaaatg ataatggttt ttacgtcaag ttgctgcgat aatttatcaa
       61 tagatgagat tatcgaacgt gctgaaaaag gcgattgcga ggctcaatat attgttgggt
      121 tttattataa tcgcgatagc gcaattgact ctccggacga cgaaaaggcc ttttactggc
      181 tgaagctggc cgctgagcaa ggtcattgtg aagcacagta ttccttaggg caaaagtata
      241 ccgaggataa aagccgtcat aaagataatg agcaagccat cttctggctg aaaaaagctg
      301 ccctacaagg ccatactttc gcttccaacg cccttggctg gacactggat cgtggagaag
      361 cccccaatta taaagaagcg gttgtctggt atcagatagc cgcggagagc ggaatgtctt
      421 atgcgcaaaa taatcttggg tggatgtaca gaaatggcaa cggagtcgca aaagactatg
      481 cgctggcatt tttctggtac aaacaagctg cattacaagg ccatagtgac gcgcaaaaca
      541 atctggccga tctttatgaa gacggaaaag gcgttgctca aaacaagaca ctcgccgcat
      601 tctggtattt gaaaagcgca cagcagggta atcggcacgc ccagtttcaa attgcatggg
      661 attataacgc tggcgaaggg gtggaccagg actataagca agcgatgtac tggtatctga
      721 aggctgccgc tcaggaaagc gtcggcgctt acgtcaacat cggttatatg tataaacacg
      781 gacaaggcgt tgagaaagat tatcaggctg cctttgaatg gtttacgaaa gccgctgaat
      841 gcaatgacgc cactgcctgg tataacctgg ccattatgta tcactacgga gaaggaagac
      901 ctgtcgatct ccgacaggct ctcgacctgt atcgtaaagt tcagtcatcc ggaaccagag
      961 atgtcagtca ggaaattcgt gagactgaag atttactgta gagataaaaa caagggccgg
     1021 ggaatttcat tcggcccata ttctctttat tgctgtgcga aatcagacta ttacggctca
     1081 aacgggcgtc tctggaactg gtcatgacca catttcgggc atagcgtcag cacttccggt
     1141 gtgtagatcg ggagatggaa gtgacatttc tcgcagacca gatttcccag cccgaccact
     1201 tctccgctgt gataaacccc atgatgattg aggtcctgga aaacttcgcg ccattcaagc
     1261 tgcgttttat cggtgatatc tgccagctcc tgccataagc tttctttaat cacccgcata
     1321 aagacgctgt cagattcttc tttcaggctc tcttcatagc tcatggcgaa ctcttccagg
     1381 tcacgtctta cagctcgcgt cagctcatcg acctcggttc gcgttaactc ccctgttttt
     1441 attacgcgct cgcgcgcctg ttccaccagt gcgtcgatat cacgttcgcc attgcgcagg
     1501 cgttcgctca gtgacgcgac cagttcacgg taatattgag caaccttgtt catcgtttca
     1561 tctcccgggt cgttaactga ctataataat agacgttctt tgttccttgc tttgctaata
     1621 cgcccacaaa ctctgtaaat taatgtatga ataatccggg catcatttca acgtcgagtg
     1681 cgcgaaaggc tgttttgacg cggacgtttg ggctatgcta tgcggatctg aaaaaccacc
     1741 tcaacgctac atttgtagcc gtattgaaaa caggaccact ggctgccatg caagagcaat
     1801 accgcccgga agagatagaa tccaaagtac agcttcattg ggatgagaag cgcacatttg
     1861 aagtaaccga agacgagagc aaagagaagt attactgcct gtctatgctt ccctatcctt
     1921 ctggtcgact acacatgggc cacgtacgta actacaccat cggtgacgtg atcgcccgct
     1981 accagcgtat gctgggcaaa aacgtcctgc agccgatcgg ctgggacgcg tttggtctgc
     2041 ctgcggaagg cgcggcggtg aaaaacaaca ccgctccggc accgtggacg tacgacaaca
     2101 tcgcgtatat gaaaaaccag ctcaaaatgc tgggctttgg ttatgactgg agccgcgagc
     2161 tggcaacctg tacgccggaa tactaccgtt gggaacagaa attcttcacc gagctgtata
     2221 aaaaaggcct ggtatataag aagacttctg cggtcaactg gtgcccgaac gaccagaccg
     2281 tactggcgaa cgaacaagtt atcgacggct gctgctggcg ctgcgatacc aaagttgaac
     2341 gtaaagagat cccgcagtgg tttatcaaaa tcactgctta cgctgacgag ctgctcaacg
     2401 atctggataa actggatcac tggccagaca ccgttaaaac catgcagcgt aactggatcg
     2461 gtcgttccga aggcgtggag atcaccttca acgttaacga ctatgacaac atgctgaccg
     2521 tttacactac ccgcccggac acctttatgg gttgtaccta cctggcggta gctgcgggtc
     2581 atccgctggc gcagaaagcg gcggaaaata atcctgaact ggcggccttt attgacgaat
     2641 gccgtaacac caaagttgcc gaagctgaaa tggcgacgat ggagaaaaaa ggcgtcgata
     2701 ctggctttaa agctgttcac ccattaacgg gcgaagaaat tcccgtttgg gcagcaaact
     2761 tcgtattgat ggagtacggc acgggcgcag ttatggcggt tccgggtcac gaccagcgcg
     2821 actacgagtt tgcctctaaa tacggcctga acatcaaacc agttatcctg gctgctgacg
     2881 gctctgagcc agatctttct cagcaagccc tgactgaaaa aggcgtgctg ttcaactctg
     2941 gcgaattcaa cggtcttgac catgaagcgg ccttcaacgc catcgccgat aaactgactg
     3001 cgatgggcgt tggcgagcgt aaagtgaact accgcctgcg cgactggggt gtttctcgtc
     3061 agcgttactg gggcgcgccg attccgatgg tgacgctgga agacggtacc gtaatgccga
     3121 ccccggacga ccagctgccg gtgatcctgc cggaagatgt ggtgatggac ggcattacca
     3181 gcccgattaa agcagatccg gagtgggcaa aaactaccgt taacggtatg ccagcgctgc
     3241 gtgaaaccga cactttcgac acctttatgg agtcctcctg gtactatgcg cgctacactt
     3301 gcccagagta caaagaaggt atgctggatt ccgaagcggc taactactgg ctgccggtgg
     3361 atatctacat tggtggtatc gaacacgcca ttatgcacct gctctacttc cgcttcttcc
     3421 acaaactgat gcgtgatgca ggcatggtga actctgacga accagcgaaa cagttgctgt
     3481 gtcagggtat ggtgctggca gatgccttct actatgttgg cgaaaacggc gaacgtaact
     3541 gggtttcccc ggttgatgct atcgttgaac gtgacgagaa aggccgtatc gtgaaagcga
     3601 aagatgcggc aggccatgaa ctggtttata ccggcatgag caaaatgtcc aagtcgaaga
     3661 acaacggtat cgacccgcag gtgatggttg aacgttacgg cgcggacacc gttcgtctgt
     3721 ttatgatgtt tgcttctccg gctgatatga ctctcgaatg gcaggaatcc ggtgtggaag
     3781 gggctaaccg cttcctgaaa cgtgtctgga aactggttta cgagcacaca gcaaaaggtg
     3841 atgttgcggc actgaacgtt gatgcgctga ctgaagatca gaaagcgctg cgtcgcgatg
     3901 tgcataaaac gatcgctaaa gtgaccgatg atatcggccg tcgtcagacc ttcaacaccg
     3961 caattgcggc gattatggag ctgatgaaca aactggcgaa agcaccaacc gatggcgagc
     4021 aggatcgcgc tctgatgcag gaagcgctgc tggccgttgt ccgtatgctt aacccgttca
     4081 ccccgcacat ctgcttcacg ctgtggcagg aactgaaagg cgaaggcgat atcgacaacg
     4141 cgccgtggcc ggttgctgac gaaaaagcga tggtggaaga ctccacgctg gtcgtggtgc
     4201 aggttaacgg taaagtccgt gccaaaatca ccgttctggt ggacgcaacg gaagaacagg
     4261 ttcgcgaacg tgctggccag gaacatctgg tagcaaaata tcttgatggc gttaccgtgc
     4321 gtaaagtgat ttacgtacca ggtaaactcc tcaatctggt cgttggctaa gcgcgggagg
     4381 aagcgtgcga tatctggcaa cattgttgtt atctctggcg gtgttaatca ccgccgggtg
     4441 tggctggcat ctgcgtgata ccacgcaggt tccttccact atgaaggtca tgatcctgga
     4501 ctcaggcgat ccgaacgggc cattaagccg tgcagtgcgt aaccagttac gtctgaatgg
     4561 tgtcgagttg cttgataaag aaaccacgcg taaggacgtt ccatccctgc gtttgggtaa
     4621 agtgagcatc gcgaaagata ccgcatcggt attccgtaac ggtcaaacag cagagtatca
     4681 gatgatcatg acggtgaatg cgaccgtgtt gatccccggc cgtgatatct acccgattag
     4741 cgccaaagtc ttccgttcgt tctttgataa cccgcaaatg gcgttagcga aagataacga
     4801 acaagacatg atcgtaaaag agatgtacga ccgtgctgcc gaacagctga ttcgtaagct
     4861 gccaagcatc cgtgctgcgg atattcgttc cgacgaagaa cagacgtcga ccacaacgga
     4921 tactccggca acgcctgcac gcgtctccac cacgctgggt aactgatgat tcggttgtac
     4981 ccggaacaac tccgcgcgca gctcaatgaa gggctgcgcg cggcgtatct tttacttggt
     5041 aacgatcctc tgttattgca ggaaagccag gacgctgttc gtcaggtagc tgcggcacaa
     5101 ggattcgaag aacaccacac tttttccatt gatcccaaca ctgactggaa tgcgatcttt
     5161 tcgttatgcc aggctatgag tctgtttgcc agtcgacaaa cgctattgct gttgttacca
     5221 gaaaacggac cgaatgcggc gatcaatgaa caacttctca cactcaccgg acttctgcat
     5281 gacgacctgc tgttgatcgt ccgcggtaat aaattaagca aagcgcaaga aaatgccgcc
     5341 tggtttactg ctcttgcgaa tcgcagcgtg caggtgacct gtcagacacc ggagcaggct
     5401 cagcttcccc gctgggttac tgcgcgcgca aaacagctca acttagaact ggatgacgcg
     5461 gcaaatcagg tgctctgcta ctgttatgaa ggtaacctgc tggcgctggc tcaggcactg
     5521 gagcgtttat cgctgctctg gccagacggc aaattgacat taccgcgcgt tgaacaggcg
     5581 gtgaatgatg ccgcgcattt cacccctttt cattgggttg atgctttgtt gatgggaaaa
     5641 agtaagcgcg cattgcatat tcttcagcaa ctgcgtctgg aaggcagcga accggttatt
     5701 ttgttgcgca cattacaacg tgaactgttg ttactggtta acctgaaacg ccagtctgcc
     5761 catacgccac tgcgtgcgtt gtttgataag catcgggtat ggcagaaccg ccggggcatg
     5821 atgggcgagg cgttaaatcg cttaagtcag acgcagttac gtcaggccgt gcaactcctg
     5881 acacgaacgg aactcaccct caaacaagat tacggtcagt cagtgtgggc agagctgaaa
     5941 gggttatctc ttctgttgtg ccataaaccc ctggcggacg tatttatcga cggttgatat
     6001 gaaatcttta caggctctgt ttggcggcac ctttgatccg gtgcactatg gtcatctaaa
     6061 acccgtggaa acgctggcga atttgattgg tctgacgcgg gtcacaatca tccctaataa
     6121 tgttcctccg catcgtcccc agccggaagc gaacagcgtg cagcgtaaac acatgcttga
     6181 actggcgatt gccgacaagc cattatttac tcttgatgaa cgcgagctaa agcgcaatgc
     6241 cccctcttac actgcgcaaa cactgaaaga gtggcggcag gaacaaggac cggacgtgcc
     6301 gctggcgttt attattggtc aggattcact gctgaccttt ccgacctggt acgaatacga
     6361 aacgatactc gacaatgcac atttgatcgt ctgtcggcgt ccaggttacc cacttgaaat
     6421 ggcgcaaccg caataccagc aatggctgga agatcatttg acacataacc cggaagatct
     6481 tcaccttcag cctgccggta aaatttatct ggctgaaacg ccgtggttta acatctcggc
     6541 gaccatcatc cgcgaacgtt tgcaaaacgg tgaatcgtgt gaggatttat tgccggaacc
     6601 ggtactgact tacattaacc aacaaggctt gtatcgctga tacctgtcgt tacggagggc
     6661 atgatgcgac tgtggttaat tcgtcatggt gaaacgcaag cgaatatcga tggtctttac
     6721 agcggtcatg cgcccacccc cctgaccgcg cgcggtattg agcaagcgca aaatctgcat
     6781 acgctgctac atggtgtttc ctttgatctg gttttatgca gtgaactgga acgggcacag
     6841 cataccgcac gactggttct cagtgaccgc cagctccccg tgcaaatcat ccctgaactc
     6901 aacgaaatgt tttttggcga ctgggagatg cgacatcatc gcgacctcat gcaagaagat
     6961 gccgaaaact atagcgcgtg gtgcaatgac tggcagcatg caatccccac gaacggtgaa
     7021 ggatttcagg cattttcgca acgtgtggaa cgctttatcg caaggcttag tgaatttcag
     7081 cactatcaga atattttagt cgtcagccat cagggtgtac tgagtctgtt aatcgcccgt
     7141 ttaattggca tgcctgccga ggctatgtgg cattttcgcg ttgactaggg gtgttggagc
     7201 gccattgata tcaaccaaaa attcgcgacg ctacgcgtcc tcaatagccg tgccatcggg
     7261 gtcgagaatg catgactttt ctgttttttt acgggtaagc cgcaacgacc attgacagac
     7321 ccgggcaggc tgatattctc cgcagccaga cttttttcgc cagacacgac tttgtagaaa
     7381 ttgttttaca aaaatggcga tgcaatctgc ggcgcggggt gggatgatag cccactttcg
     7441 aaagccgatt cggcgacaat tgtcccgaaa tcgcctctgg ttcaggtata ctgacagacc
     7501 atttttatct atttgattca cccaggggga aaacttgcag ggtaaagcac tccaggattt
     7561 tgttatcgac aaaattgatg acctcaaagg tcaggacatc atcgccttag acgttcaagg
     7621 caaatccagc atcaccgact gcatgatcat ctgtacgggt acgtccagcc gtcatgttat
     7681 gtccattgct gaccacgttg tgcaggagtc tcgcgcagcg ggcctgttac cgctcggcgt
     7741 agaaggtgaa aacagcgccg actggattgt cgtggatttg ggcgatgtga ttgtccatgt
     7801 catgcaggaa gagagccgtc gcctgtatga actggaaaaa ctctggagtt aatgcgtgaa
     7861 gctgcaactt gtcgccgtgg gaacgaaaat gccggactgg gtacaaaccg gttttaccga
     7921 gtacctgcgt cgttttccga aagatatgcc cttcgagctg attgaaattc cggccggaaa
     7981 acgcggcaag aatgcggaca tcaagcgcat actcgacaaa gagggtgagc agatgttggc
     8041 ggccgcaggc aaaaaccgca ttgtcaccct cgatattcca ggcaagccct gggatacgcc
     8101 gcagttagcc gctgagctgg aacgctggaa gctggatggt cgcgacgtca gtctactgat
     8161 tggcgggcct gaagggttgt cgcctgcctg taaagcggcg gcggagcaga gctggtcgct
     8221 gtcggcgctt accctccccc atccgctggt tcgcgtgctg gtcgcagaga gtctgtaccg
     8281 ggcgtggagc atcaccacca accatcctta tcaccgtgag tgataaggga gctttgagta
     8341 gaaaacgcag cggatgaaac tacagaactc ttttcgcgac tatacggcag agtccgcgct
     8401 gtttgtgcgc cgggcgctgg tcgccttttt ggggattttg ctgctgaccg gcgtgcttat
     8461 cgccaacctg tataatctgc aaattgttcg ctttaccgac taccagactc gctctaatga
     8521 aaaccgcatt aagctggtgc ctatcgcgcc cagccgcggc attatctacg accgtaatgg
     8581 tatccctctg gccctcaacc gcactatcta ccagatagaa atgatgccag agaaagtcga
     8641 taacgtgcag caaacgctgg acgctttgcg cagcgtggtt gatctgaccg atgacgatat
     8701 tgctgcattc cgaaaagagc gcgcacgttc acaccgtttc acctctattc cggtgaaaac
     8761 taacctgacc gaagtacaag tagctcgctt tgccgtcaat cagtaccgtt ttccgggtgt
     8821 cgaagttaaa ggctataaac gtcgttacta tccttacggt tcggcgttga cccacgtcat
     8881 cggctatgtg tcgaaaatca acgataaaga cgtcgaacgc ctgaataatg acggcaaact
     8941 ggccaactat gcggcaacgc atgatatcgg taagctgggc attgagcgtt actatgaaga
     9001 cgtgctgcac ggtcagaccg gttatgaaga ggttgaagtt aacaaccgtg ggcgtgttat
     9061 ccgccagtta aaagaagtac caccgcaagc cggacacgat atttacctga cgctggatct
     9121 caaactccag caatatattg aaacgctgct ggcgggtagc cgcgcagctg tggttgtcac
     9181 cgatccgcgt acaggtgggg tgctggcgct ggtttccacg cctagttatg acccaaacct
     9241 gtttgttgac ggtatctcca gcaaagatta ttccgccttg ttgaacgccc cgaatacacc
     9301 gctggtgaac cgcgccacac agggggttta tcctcccgcg tctacagtta aaccctatgt
     9361 ggcggtttcg gcattgagcg ccggggtgat cacgcgcaat acgacgctgt ttgacccagg
     9421 ctggtggcaa ctgccaggtt cggaaaaacg ttatcgtgac tggaaaaaat ggggccacgg
     9481 gcgtctgaat gtcacaagat cgctggaaga atctgcggat accttcttct atcaggtggc
     9541 ctacgatatg gggatcgatc gcctctccga atggatgggt aaattcggtt atggtcatta
     9601 caccggtatc gacctggcgg aagaacgttc cggcaacatg cctacccgcg aatggaaaca
     9661 gaaacgcttt aaaaaaccgt ggtatcaggg tgacaccatt ccggttggta tcggtcaggg
     9721 ttactggaca gcgaccccaa tccagatgag taaggcactg atgatcctga ttaatgacgg
     9781 tatcgtgaag gttcctcatt tgctgatgag caccgccgaa gacggcaaac aggtgccatg
     9841 ggtacagccg catgaaccgc ccgtcggcga tattcattcc ggttactggg agctggcgaa
     9901 agacggtatg tacggtgttg ctaaccgccc taacggtacg gcgcataaat actttgccag
     9961 cgcaccgtac aaaattgcgg cgaaatccgg taccgctcag gtcttcggtc tgaaagcgaa
    10021 cgaaacctat aatgcgcaca aaattgccga gcgtttacgt gaccacaaac tgatgaccgc
    10081 ctttgcgcca tacaacaatc cgcaagtggc tgtcgccatg attctggaga acggtggtgc
    10141 gggtccggcg gttggtacac tgatgcgcca gatcctcgac cacattatgc tgggtgataa
    10201 caacaccgat ctgcctgcgg aaaatccagc ggttgccgca gcggaggacc attaatcatg
    10261 acggataatc cgaataaaaa aacattctgg gataaagtcc atctcgatcc cacaatgctg
    10321 ctgatcttac tggcattgct ggtttacagc gccctggtta tctggagcgc cagcggtcag
    10381 gatattggca tgatggagcg taaaatcggc caaatcgcga tgggtctagt catcatggtg
    10441 gtgatggcgc aaattcctcc acgcgtttat gaaggctggg ccccctatct ctatatcatc
    10501 tgtattattt tgctggtggc ggtagatgct ttcggtgcca tctctaaagg tgctcaacgc
    10561 tggctggacc tcggtattgt tcgttttcag ccgtcggaaa ttgccaaaat agccgtacca
    10621 ctgatggttg cgcgctttat caaccgcgac gtttgcccgc catcgttgaa gaacactggc
    10681 atcgcgctgg tgctgatatt tatgcccacg ctgctggtgg ctgcacagcc tgacctggga
    10741 acatcaatcc tcgttgcgct ttccggtctg tttgtactgt tcctctctgg ccttagctgg
    10801 cgtctgattg gcgtcgcagt agtgctggtg gcggcgttca ttccgattct gtggttcttc
    10861 ctgatgcatg attaccagcg ccagcgcgta atgatgctcc tagacccgga atcagaccca
    10921 ctcggcgcgg gctatcacat tattcagtct aaaattgcta ttggctccgg cggattacgc
    10981 ggcaaaggct ggctgcacgg cactcagtca cagcttgaat ttctccccga acgccatacc
    11041 gactttatct tcgcggtact ggcggaagag ctgggattag tgggcattct gattctgctc
    11101 gctctctaca ttctgctgat catgcgcggg ctgtggatag ccgccagagc gcaaaccacc
    11161 tttggtcgcg tcatggctgg tggcttaatg ctgatattat tcgtttatgt cttcgtaaat
    11221 attggtatgg taagcggtat tctgccggtt gtaggggttc cgctcccact ggtcagttat
    11281 ggaggatcgg cgctaattgt gctgatggct gggttcggga ttgtaatgtc aatccacacc
    11341 cacaggaaaa tgttgtcgaa aagcgtgtaa gaggtgcgca atgcgtaagc agtggctcgg
    11401 gatctgcatc gcggcaggaa tgctcgcggc atgtacaagc gatgatggtc agcaacagac
    11461 ggtaagtgta ccgcagcctg cggtatgtaa cggccccata gttgaaatta gcggggcgga
    11521 cccgcgtttc gaaccactga acgcgacggc aaatcaggat taccagcgcg acggtaaaag
    11581 ctacaaaatc gtgcaggatc cgtctcgatt tagccaggcg ggactggcgg caatctatga
    11641 tgccgaaccc ggcagtaacc tgacggcctc tggcgaagct ttcgatccga cacagctgac
    11701 ggcggcccat ccaacgcttc cgatccccag ctacgccaga atcactaacc tggctaacgg
    11761 gcgaatgatc gtggtgcgca ttaatgatcg cggcccttac ggcaacgaca gcgttatttc
    11821 actttctcgc gcggcagctg accgtcttaa cacgtcaaac aacaccaaag ttcgtatcga
    11881 tccgattatt gtcgcccagg atggttcgct ttctggtcct ggtatggctt gtaccacagt
    11941 cgccaaacag acttacgccc tgccagcacc tcccgattta agcggtggcg cgggaacaag
    12001 ttcagtgtct ggcccgcagg gtgacattct tccggtcagt aattcgacgc taaaaagcga
    12061 agatccgacc ggcgcgccgg taaccagtag cggtttcctc ggcgctccaa cgaccttagc
    12121 gcctggtgta ctggaaggca gcgaaccgac gcctgcgcca cagcccgttg ttacagctcc
    12181 gtcgacaacg cctgcaacct cgcctgcaat ggtgacaccg caagccgcct cgcaaagcgc
    12241 cagcggcaac tttatggtgc aagtcggggc cgtaagcgat caggctcgtg cgcaacagta
    12301 ccaacagcaa ctgggacaga agttcggcgt ccccggtcgc gtaactcaaa atggcgcggt
    12361 ctggcggatc cagcttggcc cattcgccag caaagccgaa gccagtacct tgcagcaacg
    12421 tttgcaaacc gaagcccaat tacagtcatt tattactacc gcgcagtagc gtaaagcagg
    12481 catctgatgt gtcaatttct gtaaatgacg ttaacaaagt cacgcggaaa gtcagatgcc
    12541 tgccggtagt gcatttgcta tagtagggca cttttttaat tccatcacgg atgtcgtagt
    12601 tcagaccatg aataccattt tttccgctcg tatcatgaag cgcctggcgc tcaccacggc
    12661 tctttgcaca gcctttatct ctgctgcaca tgccgatgac ctgaatatca aaactatgat
    12721 cccgggtgta ccgcagatcg atgcggagtc ctacatcctg attgactata actccggcaa
    12781 agtgctcgcc gaacagaacg cagatgtccg ccgcgatcct gccagcctga ccaaaatgat
    12841 gaccagttac gttatcggcc aggcaatgaa agccggtaaa tttaaagaaa ctgatttagt
    12901 cactatcggc aacgacgcat gggccaccgg taacccggtg tttaaaggtt cttcgctgat
    12961 gttcctcaaa ccgggcatgc aggttccggt ttctcagctg atccgcggta ttaacctgca
    13021 atcgggtaac gatgcttgtg tcgccatggc cgattttgcc gctggtagcc aggacgcttt
    13081 tgttggcttg atgaacagct acgttaacgc actgggcctg aaaaataccc acttccagac
    13141 ggtacatggt ctggatgctg atggtcagta cagctccgcg cgagatatgg cgctgatcgg
    13201 ccaggcattg atccgtgacg taccgaatga atactcgatc tataaagaaa aagaatttac
    13261 gtttaacggt attcgccagc tgaaccgtaa cggcctgtta tgggataaca gcctgaatgt
    13321 cgacggcatc aaaaccggac acactgacaa agcaggttac aaccttgttg cttctgcgac
    13381 tgaaggccag atgcgcttga tttctgcggt gatgggcgga cgtactttta aaggccgtga
    13441 agccgaaagt aaaaaactgc taacctgggg cttccgtttc ttcgaaaccg ttaacccact
    13501 gaaagtaggt aaagagttcg cctctgaacc ggtttggttt ggtgattctg atcgcgcttc
    13561 gttaggggtt gataaagacg tgtacctgac cattccacgt ggtcgcatga aagatctgaa
    13621 agccagctat gtgctgaaca gcagtgaatt gcatgcgccg ctgcaaaaga atcaggtcgt
    13681 cggaactatc aacttccagc ttgatggcaa aacgatcgag caacgcccgc tggttgtgtt
    13741 gcaagaaatc ccggaaggta acttcttcgg caaaatcatt gattacatta aattaatgtt
    13801 ccatcactgg tttggttaaa aattgaacac ttgaaagtgt aatttccgtc cccatatact
    13861 aagcatcagt aaaaaactcc cgccttctgg cgggagttgc tatttaatta cgttacgccg
    13921 gagctgacat gaaaaccaaa cttaacgaac tgcttgaatt ccctactcct tttacttaca
    13981 aagttatggg gcaggcgtta cctgagctgg ttgatcaggt ggttgaagtg gtacagcgcc
    14041 atgcgccagg tgactacacc ccaacggtaa aaccaagcag caaaggcaac taccactcgg
    14101 tatctatcac tatcaacgcc actcatatcg agcaggttga aacactgtat gaagaactag
    14161 gcaaaatcga tattgtccgc atggttctgt aactcgcttc tccgttaccc ggcccctggt
    14221 cgggtaactc ccctctcctc gctgtgatat acttttcccc cacttttact cattctccac
    14281 ggagatgcag ttttgtatca ggataaaatt cttgtccgcc agctcggtct tcagccttac
    14341 gagccaatct cccaggctat gcatgaattc accgataccc gcgatgatag tacccttgat
    14401 gaaatctggc tggtcgagca ctatccggta ttcacccaag gtcaggcagg aaaagcggag
    14461 cacattttaa tgccgggtga tattccggtg atccagagcg atcgcggtgg gcaggtgact
    14521 tatcacgggc cggggcaaca ggtgatgtat gtgttgctta acctgaaacg ccgtaaactc
    14581 ggtgtgcgtg aactggtgac cttgcttgag caaacagtgg tgaataccct ggctgaactg
    14641 ggtatagaag cgcatcctcg ggctgacgcg cctggtgtct atgtcgggga aaagaaaatt
    14701 tgctcactgg gtttacgtat tcgacgcggt tgttcattcc acggtctggc attaaacgtc
    14761 aatatggatc tttcaccatt tttacgtatt aatccttgtg ggtatgccgg aatggaaatg
    14821 gctaaaatat cacaatggaa acccgaagcg acgactaata atattgcacc acgtttactg
    14881 gaaaatattt tagcgctact aaacaatcct gacttcgaat atattaccgc ttaattgcat
    14941 acctcaatgg cccaatttac actgggtcat tactcataat acctgctaat gctttacatc
    15001 tccttcgcgt ttgtatactt tctttatcac ttgcagaaat gcggtgctac gccttgctcc
    15061 gtaactcacc ccattgatta acctattgaa atagttgatt atggtgagca aaagcgttcc
    15121 gttccaatta gacataagat tattgtaatt tttttaaata ttatttttcc atgaataaaa
    15181 ttggagtaag tgcgtggata gtaataatca aattgaaccc tgtttaagca gaaagtcgtc
    15241 agaaggtaaa ccacaaatat ttacaacctt acgaaatatt gatcttaacc ttctgactat
    15301 ttttgaagct gtatatgtac ataaagggat cgttaatgca gcgaaagtgc ttaatctgac
    15361 cccctcggca atcagtcagt ctattcagaa actgcgcgtt atattccctg acccattgtt
    15421 tattcgcaaa ggccagggtg tcactcctac cgcatttgcg atgcatctac atgagtatat
    15481 cagtcagggc cttgagtcca ttcttggcgc gctggatatc gaaggaagct atgataagca
    15541 acgaacgata actattgcga ccactccctc ggtcggagcc ctggtccttc ctgtcatcta
    15601 tcgggcgatt aaaactcact atccgcagct tttactgcgc aacccgccca tcagcgacgc
    15661 ggaaaaccaa ctcagtcagt ttcaaaccga tctcatcatc gataacatgt tttgcaccaa
    15721 tcgtacggtg caacatcatg ttctgttcac cgacaatatg gtgttaattt gccgcgaggg
    15781 aaatccacta ctctctttag aagatgacag agagactatc gacaacgctg cgcatgtact
    15841 cctgttaccg gaagggcaaa atttcagcgg tctgcggcag agagttcaag agatgtttcc
    15901 ggaccggcaa atcaatttca ccagctacaa cattttgaca atcgctgcac tggttgccaa
    15961 cagtgacatg ttagcgatta ttcccagccg tttttataac ctgtttagtc gttgctggcc
    16021 gctggaaaaa ctgccttttc cgtccttaaa tgaggagcaa atagatttct ctatccacta
    16081 caacaaattc agcctgcgtg atcccatcct gcacggggta atagatgtta tccgtaatgc
    16141 attttgaaaa agtattgtta cagccagatc acagcgcaga acgataaaat ggcacaatcc
    16201 aacaacaatt ttacattttc gcgaccgctt tggctgcttt caggtccgtt tcaatgatat
    16261 actgccagtc gttaattcaa aaatagttga taattacaac aatctattga attgaaacgc
    16321 tttccttcgt aattcgcaac tggaacacgc acgctatgag taaacccatt gtgatggaac
    16381 gcggtgttaa ataccgcgat gccgataaga tggcccttat cccggttaaa aacgtggcaa
    16441 cagagcgcga agccctgctg cgcaagccgg aatggatgaa aatcaagctt ccagcggact
    16501 ctacacgtat ccagggcatc aaagccgcaa tgcgcaaaaa tggcctgcat tctgtctgcg
    16561 aggaagcctc ctgccctaac ctggcggaat gcttcaacca cggcacagca acgtttatga
    16621 tcctcggcgc tatttgtacc cgccgttgtc cgttctgtga tgttgcccac ggtcgcccgg
    16681 tagctcctga tgccaatgaa ccagtgaaac tggcgcagac cattgccgat atggcgctgc
    16741 gttatgtggt tatcacctcc gttgaccgtg atgacctgcg cgatggcggt gcccagcact
    16801 ttgcggattg cattactgcc attcgggaaa aaagcccgca aatcaaaatt gaaactctgg
    16861 tgccggattt ccgtggtcgt atggatcgtg ctctggatat tctgactgca acgccaccag
    16921 atgtgttcaa ccataacctg gaaaacgtac cgcgtattta ccgtcaggta cggcctggtg
    16981 cagattacaa ctggtcgctg aagctgctgg aacgctttaa agaagcgcat ccggaaatcc
    17041 cgaccaagtc tggtctgatg gtgggactgg gtgaaaccaa tgaagaaatt attgaggtaa
    17101 tgcgcgacct gcgccgtcat ggtgtgacga tgttaacgct ggggcaatat ttgcagccaa
    17161 gccgccatca cctgccggtt caacgttacg ttagcccgga tgagttcgac gaaatgaaag
    17221 ccgaagcgct ggcgatgggc tttacccatg ctgcatgcgg tccgtttgtc cgctcttctt
    17281 accacgccga tttgcaggcg aaagggatgg aagttaagta atcctgtaat aattacttac
    17341 atctgtctga taaaaaaccc gctttttg
//
LOCUS       DADMKF010000080        17021 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.081, whole genome shotgun sequence.
ACCESSION   DADMKF010000080 DADMKF010000000
VERSION     DADMKF010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17021
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.081"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            <1..266
                     /locus_tag="JNC24_002460"
     CDS             <1..266
                     /locus_tag="JNC24_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9159186.1"
                     /translation="TKAEAPAAQSKAAVPAKASDEEGTRVSINNASAEELARAMNGVG
                     LKKAQAIVSYREEYGPFKTVEDLKQVPGMGNSLVERNLAVLTL"
     gene            360..758
                     /locus_tag="JNC24_002461"
     CDS             360..758
                     /locus_tag="JNC24_002461"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgC/FadM family acyl-CoA thioesterase"
                     /protein_id="HAY9159187.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGLENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(810..1505)
                     /gene="queC"
                     /locus_tag="JNC24_002462"
     CDS             complement(810..1505)
                     /gene="queC"
                     /locus_tag="JNC24_002462"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HAY9159188.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(1570..3270)
                     /locus_tag="JNC24_002463"
     CDS             complement(1570..3270)
                     /locus_tag="JNC24_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
                     /protein_id="HAY9159189.1"
                     /translation="MRLLNRLNQYQRLWQPSAGKPQTVTVSELAERCFCSERHVRTLL
                     RQAQEAGWLEWQAQSGRGKRGQLRFLVTPESLRNAMMEQALETGKQQDVLELAQLAPG
                     ELRTLLQPFMGGQWQNDTPTLRIPYYRPLEPLQPGFLPGRAEQHLAGQIFSGLTRFDN
                     NTQRPIGDLAHHRETSTDGLRWDFYLRSTLHWHNGDAVKASHLHQRLLMLLQLPALDQ
                     LFISVKRIEVTHPQCLTFFLHRPDYWLAHRLASYCSHLAHPQFPLIGTGPFRLTQFTA
                     ELVRLESHDYYHLRHPLLKAVEYWITPPLFEKDLGTSCRHPVQITIGKPEELQRVSQV
                     SSGISLGFCYLTLRKSPRLSLWQARKVISIIHQSGLLQTLEVGENLITASHALLPGWT
                     IPHWQVPDEVKLPKTLTLVYHLPIELHTMAERLQATLAAEGCELTIIFHNAKNWDDTT
                     LLAHADLMMGDRLIGEAPEYTLEQWLRCDPLWPHVFDAPAYAHLQSTLDAVQVMPDEE
                     NRFNALKAVFSQLMADATLTPLFNYHYRISAPPGVNGVRLTPRGWFEFTEAWLPAPSQ
                     "
     gene            3370..4188
                     /gene="cof"
                     /locus_tag="JNC24_002464"
     CDS             3370..4188
                     /gene="cof"
                     /locus_tag="JNC24_002464"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HAY9159190.1"
                     /translation="MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKEIPALLQAFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            4253..4798
                     /locus_tag="JNC24_002465"
     CDS             4253..4798
                     /locus_tag="JNC24_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706341.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HAY9159191.1"
                     /translation="MVFKPHRINCRDFTFKIELKEKKILRGRAMLDKIDRKLLALLQQ
                     DCTLSLQALAEAVNLTTTPCWKRLKRLEDDGILIGKVALLDPEKIGLGLTAFVLIKTQ
                     HHSSEWYCRFVTVVTEMPEVLGFWRMAGEYDYLMRVQVADMKRYDEFYKRLVNSVPGL
                     SDVTSSFAMEQIKYTTSLPIE"
     gene            4828..6600
                     /locus_tag="JNC24_002466"
     CDS             4828..6600
                     /locus_tag="JNC24_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HAY9159192.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGLILMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDCVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDVLAQQSGWYRD
                     MYRYQQLEAALDDAPENHEEAVDA"
     gene            6593..8374
                     /locus_tag="JNC24_002467"
     CDS             6593..8374
                     /locus_tag="JNC24_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HAY9159193.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDY
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSSNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIRLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDGIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIVDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            8555..8893
                     /gene="glnK"
                     /locus_tag="JNC24_002468"
     CDS             8555..8893
                     /gene="glnK"
                     /locus_tag="JNC24_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HAY9159194.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            8923..10209
                     /gene="amtB"
                     /locus_tag="JNC24_002469"
     CDS             8923..10209
                     /gene="amtB"
                     /locus_tag="JNC24_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HAY9159195.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(10258..11118)
                     /gene="tesB"
                     /locus_tag="JNC24_002470"
     CDS             complement(10258..11118)
                     /gene="tesB"
                     /locus_tag="JNC24_002470"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HAY9159196.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHGIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            11336..11908
                     /locus_tag="JNC24_002471"
     CDS             11336..11908
                     /locus_tag="JNC24_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9159197.1"
                     /translation="MKLVHMASCLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINKGGTKADLTLVPMQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            complement(11939..12250)
                     /locus_tag="JNC24_002472"
     CDS             complement(11939..12250)
                     /locus_tag="JNC24_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HAY9159198.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            12413..12509
                     /gene="ffs"
                     /locus_tag="JNC24_002473"
     ncRNA           12413..12509
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="JNC24_002473"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            12629..12982
                     /locus_tag="JNC24_002474"
     CDS             12629..12982
                     /locus_tag="JNC24_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HAY9159199.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(13024..14572)
                     /gene="pdeB"
                     /locus_tag="JNC24_002475"
                     /pseudo
     CDS             complement(13024..14572)
                     /gene="pdeB"
                     /locus_tag="JNC24_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308537.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
     gene            complement(14736..15206)
                     /locus_tag="JNC24_002476"
     CDS             complement(14736..15206)
                     /locus_tag="JNC24_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9159200.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSINFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(15322..15873)
                     /gene="maa"
                     /locus_tag="JNC24_002477"
     CDS             complement(15322..15873)
                     /gene="maa"
                     /locus_tag="JNC24_002477"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HAY9159201.1"
                     /translation="MSTEKEKMIAGELYRSADETLSRDRLRARQLIHRYNHSLAEEHT
                     LRQQILADLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKDVPDNVVVGGNPARIIKKL"
     gene            complement(16044..16262)
                     /gene="hha"
                     /locus_tag="JNC24_002478"
     CDS             complement(16044..16262)
                     /gene="hha"
                     /locus_tag="JNC24_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HAY9159202.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(16288..16662)
                     /gene="tomB"
                     /locus_tag="JNC24_002479"
     CDS             complement(16288..16662)
                     /gene="tomB"
                     /locus_tag="JNC24_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HAY9159203.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIETFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQDLQKWRK
                     SGNRLFRCFVNATKENPASLSC"
BASE COUNT         4066 a         4123 c         4557 g         4275 t
ORIGIN      
        1 aaaccaaagc ggaagctcct gcagcacaaa gtaaagcagc agtaccggcg aaagccagtg
       61 acgaagaagg cacccgggtc agcattaata atgccagcgc ggaagagcta gcccgcgcga
      121 tgaatggcgt tggcctgaag aaagcgcagg cgattgtcag ttatcgcgaa gagtacggtc
      181 cgtttaaaac ggtggaggat ctaaagcagg tgccggggat gggcaattcg ctggtggaac
      241 gtaatctggc ggtattaacc ctgtaattaa tttgcatagt ggcaattttt tgccagactg
      301 aagaggtcat accagttatg acctctgtac ttataacaac aacgtaaggt tattgcgcta
      361 tgcaaacaca aatcaaagtt cgtggatatc atctcgacgt ttaccagcac gtcaacaacg
      421 cccgctacct tgaatttctc gaagaagccc gctgggatgg gttggaaaat agcgacagtt
      481 ttcagtggat gacggcccat aacatcgcct tcgtcgtggt caatatcaat attaactatc
      541 gtcgcccagc ggtattaagc gacctgttaa ctattaccag tcagttgcag caattaaacg
      601 gtaaaagcgg catcttaagc caggtcatta cactggagcc ggaagggcag gtggtagcgg
      661 atgcgcttat tacgtttgtt tgtattgatc ttaaaacgca gaaagcatta gctctggaag
      721 gggaattgcg cgaaaagctg gagcagatgg ttaagtaaac gttttgtggt gccggatgct
      781 caagccgcat ccggcgacac ccggaataat tacctcaacc cggttttctg cttcatcgct
      841 gccatcaccg tcggtttatc ggccagataa tgattcaaac cgttggcgcg taaattacat
      901 gccgcacaat gaccgcaacc gtcgccttta atgccgttat agcaggtcaa cgtttcgtta
      961 cggactaaat ccagtttgcc gtaataatct gccagcgccc aggtttccgc tttatcaatc
     1021 cacatcagcg gcgtttcaaa acgaatatct ttcgccatgc ccaaactgac ggcatggttt
     1081 agtgctttca caaactcatc gcggcaatcc gggtagccgg agaaatccgt ttcacagacg
     1141 ccggtaatta cggcttctgc ttttacctga tacgcatata ttgccgccag cgtcaggaac
     1201 aaaatattac gcccagggac aaacgtattc gggatgccat cggcttcagg ttcataatca
     1261 ggcaccggaa tgctgtcacg tgtcaggctg ctgaccgcca gctcgttgag cagcgttaca
     1321 tcaagaacct tatgcgcgcg tgctcccagt ttcagcgcca gttcgcgtgc cacgtcgatt
     1381 tctgcgcgat gtcgctgacc gtaatcgaac gtcacgcaat ggacttcatc atattgttgt
     1441 aatgcctgca ccagacaggt ggtggaatcc tggcctccac tgaacacaac gacagcacgt
     1501 ttcatagata atcctgcttg aacaataaaa gcgttatggt aacgcctgcg attaacccgg
     1561 accagctatt cattgcgacg gcgcgggaag ccaggcttcg gtaaattcaa accagccgcg
     1621 cggtgtcagt cgcacaccgt tcacgccggg aggggcacta atgcgatagt gatagttgaa
     1681 cagcggcgtc agcgtcgcgt ctgccattaa ctggctaaaa accgctttca gggcattaaa
     1741 tcggttttcc tcatcaggca ttacttgcac cgcgtccagc gtcgattgca gatgtgcata
     1801 tgctggagcg tcgaaaacat gtggccatag tggatcgcaa cgtagccatt gctccagagt
     1861 atattccggt gcttcgccaa ttaatctgtc gcccatcatg aggtctgcgt gtgccagtag
     1921 ggtcgtgtcg tcccagtttt ttgcgttatg aaaaataatt gtgagttcac agccttccgc
     1981 tgccagtgtc gcctgtaggc gttctgccat ggtatgaagt tctatcggta ggtgataaac
     2041 cagcgtcaag gttttcggta gtttgacttc atccggtact tgccagtgcg gaatagtcca
     2101 gcctggcagt aatgcatgac tggcggtgat caggttttct ccgacttcta acgtttgtaa
     2161 taaaccggat tgatgaataa tggagatcac ttttcgcgcc tgccagaggg agagtcgggg
     2221 acttttgcgc aacgttaaat agcaaaaacc taaactgatg ccactactga cctggctgac
     2281 ccgttgcagc tcctccggtt tgccgatggt gatttgcacg ggatgccgac aactggttcc
     2341 caaatctttt tcgaaaagcg gcggagttat ccagtactca accgctttaa gcagcggatg
     2401 acgcaaatgg taataatcat ggctctccag gcgcaccagc tccgctgtga attgtgttaa
     2461 tcgaaaagga ccagtgccga tcagtgggaa ttgcggatgc gccagatggc tgcaatagct
     2521 cgccagccgg tgcgcaagcc agtaatcggg gcgatgtaaa aagaaggtca gacactgcgg
     2581 atgggtgact tcaatacgct tcacgctaat aaataattga tccagtgctg gcagttgtaa
     2641 cagcatcaat aatcgctggt gtaagtgtga ggcttttact gcatcgccgt tatgccagtg
     2701 tagggttgaa cgaagataaa agtcccagcg taacccgtca gtagaggttt ccctgtgatg
     2761 cgctaaatcg ccaatcgggc gctgagtatt attatcgaag cgggtcaggc cggaaaatat
     2821 ctgcccggcg agatgctgct cggcacggcc gggcaaaaag cctggttgta gcggttcgag
     2881 cgggcgatag tagggaatac gcaacgtggg tgtatcgttt tgccattgtc cgcccataaa
     2941 cggctgtaac agagtgcgca gctcacctgg ggccagttgc gccagctcca gcacatcttg
     3001 ctgctttccg gtttccagtg cctgttccat catcgcattg cgtagcgatt ccggcgtgac
     3061 cagaaagcgt aattgtccgc gctttccgcg tcctgactgc gcctgccact ccagccatcc
     3121 cgcctcctgt gcctgacgca acagcgtacg aacatggcgt tcgctgcaaa aacagcgttc
     3181 ggccagttcg ctgacggtga cggtttgcgg ctttccggct gaaggttgcc acagacgttg
     3241 atactggtta agacggttga gcaatcgcat ataaacccgg aacaatatta tttaactatt
     3301 cactattact tccgtatata tcaggtgata ctcaatctcc attaaccgtg tcacagagtg
     3361 gagaaagaaa tggctcgtct ggcagcattt gatatggatg gcactttatt gatgcctgac
     3421 catcatttag gtgagaaaac cctctctact ttggcgcgac tgcgtgaacg cgacattacc
     3481 ctcacttttg ccacggggcg tcatgcgctg gagatgcagc atattctcgg ggcgctatcg
     3541 ctggatgcat atttgattac cggcaacgga acgcgcgtgc attctctgga aggtgaactt
     3601 ttacatcgtg atgatttacc tgcggatgtc gcggagctgg tgctgtatca gcaatgggat
     3661 acccgagcca gcatgcatat cttcaatgac gacggttggt ttaccgggaa agagatccct
     3721 gcgttgttgc aggcatttgt ctatagcggt tttcgttatc agataatcga tgtcaaaaaa
     3781 atgccactcg gcagcgtcac caagatctgc ttctgtggcg atcacgacga tcttacacgc
     3841 ttgcagatcc agctatacga agcattaggc gagcgtgcac atttgtgttt ttccgccaca
     3901 gattgcctcg aagtgctgcc ggtgggctgc aataaaggcg ctgcattgac ggtgctgacc
     3961 caacatttag gtttatcgtt gcgcgattgc atggcctttg gtgatgcgat gaacgatcgc
     4021 gaaatgttag gcagcgtcgg tagcgggttt attatgggca atgcgatgcc gcaactgcgc
     4081 gcggagctcc cgcatttacc ggtgattgga cattgccgaa atcaggctgt ctctcactat
     4141 ttgacgcact ggctggacta tccacatcta ccttattccc ccgaataacg agatcccttc
     4201 cagcaccggg caattgcccg gtttttttgc gttgaatttg tcattttgtg ccgtggtgtt
     4261 taaaccgcac agaataaatt gtcgtgattt cacctttaaa atagaattaa aagagaaaaa
     4321 aattctccgt ggaagggcta tgttagataa aattgaccgt aagctgctgg ccttactgca
     4381 gcaggattgc accctctctt tgcaggcact ggctgaagcc gttaatctga caaccacccc
     4441 ttgctggaag cgcctgaaac ggctggagga cgacggtatc cttatcggca aagtcgccct
     4501 gctggatccg gaaaaaatag gcctcggcct gaccgccttt gtgctgataa aaacgcaaca
     4561 tcacagcagc gaatggtatt gccgctttgt cacggtggtt accgaaatgc cagaagtgct
     4621 ggggttctgg cgcatggctg gtgaatacga ttatctgatg cgcgtccagg ttgccgacat
     4681 gaaacgctac gacgagtttt ataagcgtct ggtaaacagc gtgccggggc tgtcggacgt
     4741 cacttccagc ttcgcgatgg aacagattaa atacaccact tctttaccca tcgaataaat
     4801 atccagaatc aggtcaggac acaacgcgtg cgattatttg ctcaattaag ctggtatttc
     4861 cgtcgggaat ggcgtcgcta tctcggggct gtcgccttgc ttgtcattat cgcgatgctg
     4921 caactggttc cgccaaaagt ggttggtatt gttgtcgatg gcgtgacaga acaacacttt
     4981 actaccgggc tgatcctgat gtggatcgcc accatggtgc tgattgccgt tgtggtttat
     5041 ctcctgcgtt atgtctggcg cgtattgctg tttggcgcgt cttaccaact ggctgttgaa
     5101 ctgcgtgaag attattaccg tcagctaagc cggcagcacc cggagtttta cctgcgtcat
     5161 cgcacgggtg atctcatggc tcgtgcgacc aatgacgtcg attgcgtcgt gtttgccgcc
     5221 ggagaagggg tgctgacgct ggtggattca ctggtaatgg gctgcgctgt gttgattatg
     5281 atgtctacgc aaattagctg gcagttgacc ttatttgccc tgttgccgat gccagtgatg
     5341 gcgatcatga ttaagcgcaa cggcgatgct ttgcatgaac gctttaagct ggcacaggcg
     5401 gcgttttcca gtcttaatga ccgcacccag gaaagcctca ccagtatccg catgatcaaa
     5461 gcctttggtc tggaagatcg ccagtcggcg ttatttgccg cggatgccga agataccggc
     5521 aaaaaaaaca tgcgggtggc gcgtattgat gctcgtttcg acccgaccat ctatatcgcg
     5581 attggtatgg cgaacttgct ggcgattggc ggcggtagtt ggatggtggt gcagggcagt
     5641 ttaacgctgg gccagctcac cagttttatg atgtatttag gtctgatgat ttggccaatg
     5701 ctggcgctgg catggatgtt taacattgtg gaacgtggta gtgctgcgta cagccgtatt
     5761 cgtgcgatgc tggcggaagc gccggtggtg aacgatggta gcgaacccgt gccggaaggg
     5821 cgtggcgaac ttgatgtaaa tattcaccag ttcacgtatc cgcagactga ccatcctgcg
     5881 ctggaaaacg tcaatttcgc cctgaaaccc ggtcagatgc tgggtatctg cgggccgact
     5941 ggttccggca aaagtaccct gttgtcgctc attcagcgtc atttcgacgt tagcgagggg
     6001 gatattcgct ttcatgatat tcctctgacg aagttacaac tcgatagctg gcgtagccgc
     6061 ctggcggtgg tcagccagac gccattcctt ttttctgaca ccgtggcaaa taacatcgcg
     6121 ctgggttgcc cgaatgccac ccagcaagag attgagcatg tcgcgcggtt agccagcgta
     6181 catgacgata ttttgcgttt accgcaaggt tacgatacag aggtgggcga gcgcggcgtg
     6241 atgctttccg gcgggcaaaa acagcgtatc tctattgctc gtgcgttatt agtcaacgcg
     6301 gaaatcctca tccttgatga tgcgctttcg gcggtggacg gacgcacaga gcaccagatc
     6361 ctgcataacc tgcgtcagtg gggacaggga agaacggtaa tcatcagtgc ccatcgcctt
     6421 tcggcactga cggaagccag tgaaattatt gtgatgcagc acggacatat cgcccagcgt
     6481 ggcaatcatg atgtgctggc gcaacaaagc ggctggtatc gcgatatgta tcgctatcaa
     6541 caactggagg cggcgctcga cgacgctccg gaaaatcacg aggaggccgt cgatgcgtag
     6601 ttttagccaa ctgtggccga ctctcaagcg cctgttagcg tacggttcac cgtggcgtaa
     6661 accgctgggg attgcggtcc tgatgatgtg ggttgcggcg gcggcagaag tcagtgggcc
     6721 gctgcttatc agctatttta tcgacaatat ggtagcgaaa aataacctgc cgttgaaagt
     6781 ggttgcaggg ctggctgcgg cgtatgttgg gctacaactg tttgccgccg ggctacatta
     6841 cgcgcagtcg ctgctgttta atcgggcggc agtaggcgta gtgcaacagt tgcgtaccga
     6901 cgtgatggat gctgcgttac gccagccatt aagcgagttt gatacccaac ccgtcgggca
     6961 ggtgatttcc cgcgtcacta atgacactga agtgatccgc gatctctacg ttaccgtagt
     7021 ggcaactgtc ctgcgcagtg ccgcactggt gggcgcgatg ctggtggcga tgttcagcct
     7081 cgactggcga atggcgctgg tggcgataat gattttcccg gtagtgctgg tggtaatggt
     7141 gatatatcaa cgttacagca cgccgattgt ccgtcgtgtg cgcgcctatc tggcggatat
     7201 caacgacggc tttaacgaaa tcatcaatgg catgagcgtc atccagcagt tccgtcagca
     7261 ggcgcgattt ggcgaacgta tgggtgaggc cagtcgttca cactatatgg cgaggatgca
     7321 aaccctgcgc ctcgacggtt ttctgctgcg tccgctgctg agtctgtttt catcgctcat
     7381 tctttgtgga ttgttgatgc tgtttggctt ctccgccagc ggcaccattg aagtgggcgt
     7441 gctgtatgcc tttatcagct atcttgggcg acttaacgaa cccttaatcg aactgaccac
     7501 gcaacaggcg atgctgcaac aggctgttgt tgctggtgag cgcgtgtttg aactgatgga
     7561 cggaccgcgc cagcaatatg gcaatgatga ttacccgtta cagagtggca ccatcgaagt
     7621 cgataacgtg tcatttgctt atcgcgatga caatctggtg ctaaagaaca ttaatctctc
     7681 tgtgccttcg agtaactttg tggcgctggt cgggcatacc ggcagtggta aaagtaccct
     7741 cgccagttta ttgatgggct attacccgct aacggaaggt gagattcgcc ttgatggtcg
     7801 tccattaagt tcgctaagtc acagcgcgct gcgccagggc gtggcaatgg tgcagcaaga
     7861 tccggtggtg ctggcggata ccttcctcgc caacgtgacg ctggggcggg atatctccga
     7921 agaacgcgtc tggcaggcgc tggaaaccgt gcaactggcg gagctggcgc gtagcatgag
     7981 cgacggtatt tacacgccgc tgggcgagca ggggaataat ctctcagttg ggcaaaagca
     8041 actgctggca ctggcgcgcg tgctggtcga gacgccgcaa atcctgatcc ttgatgaggc
     8101 aaccgccagc attgactccg ggactgaaca ggcgattcaa catgctctgg cggcggtgcg
     8161 tgaacatacc acgctggtgg tgattgctca ccgcttatcg accattgttg atgccgacac
     8221 cattctggtg cttcatcgtg ggcaagccgt ggagcagggc actcaccagc aactgctggc
     8281 tgcccaggga cgctactggc agatgtatca actgcaactt gcgggcgaag agctggcagc
     8341 cagcgtgcgt gaagaggaat cattgagcgc ctgaatagcg caatatttca tcgttggtgc
     8401 aaaaatgtaa cgcactgtgc actgtcatag tgcgttttca ttttcaaact tcttaacttc
     8461 ctgctctctt tctcgttttt catttctggc acaccgcttg caataccttc ttcgtgtagc
     8521 agaaccatta ccgaattctg accggagggg atctatgaag ctggtgaccg tgataatcaa
     8581 accattcaag ctggaagacg ttcgtgaagc gttatcttcc attggtattc agggcctgac
     8641 cgtcaccgaa gtgaaaggtt tcgggcgtca gaaagggcat gccgagctgt accggggggc
     8701 ggaatacagc gtcaacttcc tgccaaaagt aaaaattgat gtggcgattg ctgatgacca
     8761 actcgatgaa gtgatcgata tcgtcagtaa ggcggcttac accggaaaaa ttggcgacgg
     8821 caaaatcttc gtcgctgaat tgcaacgcgt cattcgtatt cgtaccggcg aagccgacga
     8881 agcggcgctg taatctctgg cacacagcaa caggaacgaa aaatgaagat agcgacgata
     8941 aaaactgggc ttgcttcact ggcgatgctg ccgggactgg taatggctgc acctgcggtg
     9001 gccgataaag ccgacaatgc gtttatgatg atttgtactg cgctggtgct gtttatgact
     9061 attccgggga ttgccctgtt ttacggtggg ttgattcgcg gcaaaaacgt gctgtcgatg
     9121 ctgacgcagg tgacggtgac gtttgcactg gtctgtattc tctgggtggt ttacggttac
     9181 tcgctggcgt ttggtgaggg caacaacttc ttcggcaaca ttaactggtt gatgctaaaa
     9241 aacatcgaac tgacggcggt gatgggcagc atttatcagt atatccacgt ggcgtttcag
     9301 ggctcgtttg cctgcattac cgtcggcttg atcgttgggg cgctggcgga acgaatccgc
     9361 ttctcagctg tgttgatttt cgtggtggta tggctgacgc tctcttacat tccgattgcg
     9421 catatggtgt ggggcggtgg tttgctggct tctcacggtg cgctggattt cgcgggtggc
     9481 acggtggtgc atattaacgc cgcaatcgcc ggtctggtgg gcgcgtatct gataggcaaa
     9541 cgcgtgggct tcggcaaaga ggcgtttaaa ccgcacaacc tgccaatggt cttcaccggt
     9601 actgccattc tctatattgg ttggtttggc tttaacgccg ggtcagcggg cacggcgaat
     9661 gaaatcgcgg cactggcatt tgtgaatact gtggtcgcaa cggcggcggc aattcttggc
     9721 tggatcttcg gtgaatgggc gctgcgtggt aagccttctc tgctgggagc gtgttctggc
     9781 gcgattgccg gtctggtcgg cgtgacgcca gcgtgtggct acattggggt tggcggcgcg
     9841 ttgattatcg gcgtggtagc tggtctggcg ggcttgtggg gcgttactat gctcaaacgc
     9901 ttgctgcggg tggatgatcc ctgcgatgtc ttcggtgtgc acggcgtttg tggcattgtc
     9961 ggctgtatca tgactgggat ttttgccgcc agttcgctgg gtggcgtggg cttcgctgaa
    10021 ggtgtgacga tgggccatca gttgctggta cagctggaaa gcatcgccat tacgatcgtc
    10081 tggtccggtg tggtggcatt tattggctac aaactggcgg atctgacggt tggtctgcgt
    10141 gtgccggaag aacaggagcg agaagggctg gacgtcaaca gccacggcga gaatgcctat
    10201 aacgcgtaac aagcactgca aaaaacagcc ggacggtttt cacctccggc tattttttta
    10261 attgtgatta cgcatcaccc cttcctgaac ggtcgaggca accagcacgc cgtcctgagt
    10321 ataaaactca ccgcgcacaa agccgcgtgc gctggacgcc gaggtgctct ccacgctata
    10381 cagcagccat tcattcaaat taaacgggcg atgaaaccac atggaatggt caatggtggc
    10441 aatctgaatc cccggttcga gaaaaccgat gccgtgcggc tgtagagcta ccggcaggaa
    10501 gttaagatca gaagcgtaac cgagcagata ctgatgaacg cgcaggtcat ccggcacgct
    10561 accatttgcg cggatccaca cctgacgatg tggttctgcg acgtgacctt tcagtgggtt
    10621 atgaaactcc accggacgga cttccagcgg acgatcgcag atgaatttat ctttcagcac
    10681 tggcggcagc aggtgcgcca gcgattgggc gatttgtgtt tccgaaggca ggccatcagg
    10741 cgctggcgcg gacggcattg ttttttgatg ttcgaaaccc gcttcaggtg cctggaaaga
    10801 ggccgtcata taaaaaatcg gtttgccgtt ttgaatagca gcaacccggc gggcgctgaa
    10861 gctgttaccg tcacgcagcg tttcgacatc ataaataatc ggcttcttac tatcgccagg
    10921 gcgaagaaag tagctgtgaa acgaatgtac cagccgctct tcagggacgg tctcttttgc
    10981 agcatacaag gcctgaccca cgacctggcc gccaaacacc tggcgtaaac ctaaatcttc
    11041 actctggccg cgaaagagtc cttcctcaat tttttccaga tttaacaatg tcagtaaatt
    11101 ttttagcgcc tgactcatat aactctccag taactaagct gccgcagcaa gccaaagtga
    11161 gttgagtata acgcaaattt gccactggtc cgatgggtgc aatagtctga attacgggct
    11221 aattacaggc agaaatgcgt gatgtgtgcc acacttgttg acgttactat tttgttaacc
    11281 actcttccgg cgaggaaagt tagcccgctg gtgcattgat aataaggaga agtgaatgaa
    11341 actcgtgcac atggccagtt gtttagcggt tgcgattgcg ttggcggctt gcgcagataa
    11401 aagcgcggat attcagacgc cagcaccggc ggcaaatacg tctatttcag caacacaaca
    11461 acctgctatc cagcaaccga atgtctccgg taccgtttgg atccgtcaga aagtcgcact
    11521 gccgcctgat gctgtgctga ccgtgacact ttctgacgcg tcgttagccg atgcgccgtc
    11581 aaaagtgctg gcgcagaaag cggtgcgtac cgaaggtaaa cagtcaccat tcagctttgt
    11641 tctgcctttt aacccggcag atgttcagcc gaacgcgcgt attctgttga gtgcggcgat
    11701 taccgtgaat gacaaactgg tatttattac cgataccgtt cagccggtga tcaacaaggg
    11761 cggaactaaa gccgacctga cattggtgcc gatgcagcaa accgccgtgc cggttcaggc
    11821 cagcggtggt gcaacgacta ccgtaccttc gacttcacca actcaggtga atccgtcttc
    11881 ggcagttcca gctcctacgc aatattaagc gacgttaacc ctctccgccc ggagagggtt
    11941 agtagttcca gcgataacgc tgcaagtcga tttgcccgct tcccgatacc atcacacctt
    12001 ctgccagtaa tgcctgtcgc tgacgctgta aatccggtcc ggttagcgaa attgtgccgt
    12061 ggcgattaac cacccggtgc cagggtaagg tgctgccttc agggagacgc tttaacacac
    12121 cgcccacctg gcgcgcggcg cggggcgatc ccgccagttt cgccacatca ccgtaagtgg
    12181 tgacatagcc ttcgggaata gcggcgacga tttgccagac gcgttgggga aatgaatctt
    12241 ctttttccat cttttcttcc tgaggtaatt tttcagcata atctggaaaa acgcccgagt
    12301 gaagtcgcat tgcgcaagaa accagcatct ggcacgcgat gggttgcaat tagccggggc
    12361 agcagtgata atgcgcctgc gcgttggttc tcaacgctct caatgggggc tctgttggtt
    12421 ctcccgcaac gctactctgt ttaccaggtc aggtccggaa ggaagcagcc aaggcagatg
    12481 acgcgtgtgc cgggatgtag ctggcagggc ccccacccat ttctgcctcc caccgtttcg
    12541 tcaaaaaatc ccaacatagc taaactttaa ccacaactga cgtcgcaaga attgtctggc
    12601 tgcgcagtac gcttcggagg tatgtctgat gaagtatgtt gatggttttg tggttgccgt
    12661 tcctgccgat aaaaaggatg cctatcggga aatggccgct aaggctgcgc cattgtttaa
    12721 agagtttggc gcgcttcgta ttgtcgaatg ctgggccagc gatgtaccgg atggcaaagt
    12781 gaccgatttt cgtatggcgg tgaaagcgga agagaatgaa gaggttgtct ttagctggat
    12841 tgaataccct tcaaaagagg tccgcgacgc tgctaatcaa aagatgatgt cggacccacg
    12901 gatgaaagag ttcggcgagt ccatgccgtt tgacggcaag cgaatgatct atggcggatt
    12961 cgagtcaatc atcgacgaat aggtagcgtg acgggctgtg cggcgtgccg tcagcccaaa
    13021 aattcacaaa tgttgctcgg cccagcgtaa gaaatcttct ttcggtaatg ccttgctgta
    13081 gagccagccc tgaccgtagt gcacgccatg ctggcgtagc cactcttctt gtttactggt
    13141 ttcgattccc tccgctacca ttttcagttt cagtgttttt gccatttcga tgatatgcgg
    13201 cgtgacattt ttatattcca gcgcatcaac gaaagattta tcgatcttca gaatgtcgac
    13261 atccagatcc tgtaaataac ttaaacttga atatcccgta ccaaaatcat caagataaat
    13321 ttcatggccc gcctcccggt agcgagaaat tatcggggcg ctggttttcg gatcggcaaa
    13381 ctcgcgttca gtgagttcaa gcgcgatctg tctgggatta acctgattga ttgatcattt
    13441 cacgcagcaa ttgcgggatt ttttccgagg tgagcacggt ggattcaaga ttgatcgaaa
    13501 tatgctgctg tggatgctga cgcagccagt cgcccatatc ttcaaaggcg cttcttataa
    13561 tcagtagcgt caatggctca gaaaggcccg tttgctgtgc cagcggaata aaactatctg
    13621 gtgacaacca actaccgtct gtctgcggcc agcgcgccag tgcctcagca ccgacaattt
    13681 tgccattggc taaggagaca atcggctgat agtgcacgca aatatcacga ttttcgatag
    13741 catcctgcag ccgatgatgc ggtgactgga tacggcgcaa aatacgcagc acaaacatcg
    13801 ctgccagcag gccaatcacc aacccgagcg gtaaccaaat aaagacttgc cgatgccagc
    13861 ctttctgcaa cattttcgtt gaagcccagg tgatgatcga aatattcatc tccgggaagg
    13921 gcaggatatc gtaaatgatt ccattatttt cgatatgctc accgggtgtt ttttgtagcc
    13981 tggtaataat tccctgagca atttcatcgc tgctggttat gacaacgtta tgggcattgc
    14041 caataatggc ggcatcaatt tgccatgagc tatagggaat gacatcaata aaggaagcgg
    14101 ggtcgatcat gacgacataa tgtgccgttc ccatggcgac catgtaacgg ataatgccta
    14161 aatcgttatg cgatgttaac cagacacgat aaccatcttt cgaaatttta cctggctcgg
    14221 ggaaggtatc gggcggactt tcatgctcca gagacgaaca ctggggaacg ttgttatcga
    14281 tataaaccac ttcctgaata tagcgataac tgtaagatac ccgacgcatt tccatgagat
    14341 gggcttcgct acaggcagcg ccttgccatc tttccagctc ttgcagcgca tctttccctt
    14401 gtgtcgccac cttattggcc cgaatagcga cgcgggagga ataggtatcc agctcttcaa
    14461 taaacgatgt ttctacctgc tgatgggcca gccagatgct taagccgaca ggcagcaata
    14521 ctgaaagaat cagtactccc gaaataaggc cgaccagatg tcgtgttctc accagcatat
    14581 ccttattaac acagtgtagg gaaagtatat ctgattgtgg aaaaaggctg gcaggagata
    14641 aggaggtaga gctggtattt catgaagtga tgggtggaaa cgatcattgt catttgacca
    14701 agcgcagagc tttacgcctg gtatgtaacc ctaacttaag atgttgattc ggcgcgagcg
    14761 agggtaaaaa tatcgtaaaa agacagttca cctttacgga cgatcatttt ttgcagttgt
    14821 tccttatgaa catccgcgac acgcggagag ttcaagatgg cttcaaccag cgcggcaggt
    14881 acaaagcgcg tgttgtacca ttcgccgtta tagcaaacgc ggaaatccag cacgtcgata
    14941 tcttcatagc ggtagcgtgg gtagacatcg aaaaagaaga aaccattaag caggataaat
    15001 aatacaacca gtagccagac agagcctgac agcgtttcgg actgcaacat caccgccagg
    15061 gtggcaaaaa aagcaacgta cataccgata aacagccccg gatgtttacg gataaaattg
    15121 atactaaagc ggggtttgtt gtcgcgtttt tcgcgggtat tcagagactc aatcgtttcg
    15181 gtcagcaggc gttgtatttc ggtcatcgtt tgccttcgtg gtatctgcaa ctttccaggg
    15241 aacaccctat tttaacgtgg gaatagtaga ggggaagtaa cagatttacc acaattttgc
    15301 ataacagttg cgaaaaaccg attacaattt tttaattatt ctggctggat taccgcccac
    15361 gacaacgttg tccgggacat cttttgtgac aactgcacct gaggctacca cgacgttatc
    15421 accaatggtc acaccagggt taatgaccgc gcgtccgcca atccagacgt tattaccgat
    15481 ggtgacgggt ttccccagtt cagcaccgct attacgtgct acagggtcga tgggatgtgt
    15541 tgccgtgtag atatgaacgc ctggtgccaa catacagtta tcaccgatgc gaatagggca
    15601 gacatcaagc atcacgcaat cgaagttggc gaaaaaatta ttaccgagaa aaatgttata
    15661 gccatagtca cagcgaaacg ttggctcaat ataagcctct gtcacctgac cgaatagatc
    15721 agcgagaatt tgctggcgta atgtgtgctc ttccgccagg gaatgattgt atcggtgaat
    15781 aagctgacga gcgcgcaggc gatcgcgaga taacgtctca tctgccgagc gatacaactc
    15841 accagcaatc atcttttctt tttctgtgct cattactgac ctccggttgc tgaaaatgcg
    15901 gcaacacagt aagacgaaaa gtcagtgaag agaacgttcc cattacatct gtgtgataaa
    15961 gatcacatag ggtatgtctt catggtgaaa agtataaaat tcttaataaa cagccggtta
    16021 tagctccgaa agcgaattat ttattagcga ataaatttcc atactgagga agggatcttg
    16081 tcgtatagtt tattcatggt caattcggcg aggcggtgat ctgcggctga gtaaaatacc
    16141 gccagttcat tatctgataa ttcgtattta tttttctcga taacacgctc cagcgtgtca
    16201 attgtctggc aacgacgtaa acgcattaaa taatcggttt tcgttaaagg tttttcggac
    16261 atacttctac ccatggttgt aataattcta acaagataaa ctcgcaggat tctctttcgt
    16321 cgcattgaca aaacaacgga atagtcgatt acctgacttc cgccatttct gaagatcctg
    16381 catattaata ccataactac tgaacaacat aaaggtgtca tccagatatt cgtcgatctg
    16441 ctcaatgagc ttattgtctt cattatactt aattttgtaa ttaagtgcga aggtctcaat
    16501 atgctcaatc agttcattca actggaggtt gatcgccgag gttgggtcgt taacccagcc
    16561 atgattgctt tcttcaaggt ttgcaaggca gtcatgatac agggtttcac agagaaactt
    16621 aagctgtgcg atatcatgtc ttttgggtga gtattcatcc ataacgcgtc cccttcttag
    16681 cggttgaact aacggacacc tttcgggatg gaaaaaactt actgacctgg acttgccctc
    16741 tcgtttgtta gcttaactat aagccactct ttgcaggttt tcatcgcatt attacgaaaa
    16801 attacaatta ggaaggaatc gggacaggac tcactttttc atactagcac taacccgcag
    16861 caggtaaaag cagtctgctc gaactgttca taacatttca ggattatcgc cagatgaaat
    16921 tggtgacact aataccagga ttgctctgaa tatgacgtaa taaccgagga atgaataaag
    16981 aattaccgca aataattaag aatagcctct aaatgattat g
//
LOCUS       DADMKF010000081        16873 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.082, whole genome shotgun sequence.
ACCESSION   DADMKF010000081 DADMKF010000000
VERSION     DADMKF010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16873
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.082"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            315..1304
                     /locus_tag="JNC24_002480"
     CDS             315..1304
                     /locus_tag="JNC24_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005763450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 family protein"
                     /protein_id="HAY9159204.1"
                     /translation="MSGGFVLESSGQSEYFVAGHVLISLAAICLALFTTAFIIISQLT
                     RGVNTFYNTLFPIIGYAGSIITMIWGWALLAGNDVMADEFVAGHVIFGVGMIAACVST
                     VAASSGHFLLIPKNAAGSKSDGTPVQAYSSLIGNCLIAVPVLLTLLGFIWSITLLRSA
                     DITPHYVAGHVLLGLTAICACLIGLVATIVHQTRNTFSTKEHWLWCYWVIFLGSITVL
                     QGIYVLVSSDASARLAPGIILICLGMICYSIFSKVWLLALVWRRTCSLANRIPMIPVF
                     TCLFCLFLASFLAEMAQTDMGYFIPSRVLVGLGAVCFTLFSIVSILEAGSAKK"
     gene            complement(1620..2822)
                     /locus_tag="JNC24_002481"
     CDS             complement(1620..2822)
                     /locus_tag="JNC24_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAY9159205.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKKPGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAEDETGFTLDLNGMTVGCEKLVMATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGIVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPNVSLKQLNVRDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLIAAKSS"
     gene            3054..4553
                     /gene="pitA"
                     /locus_tag="JNC24_002482"
     CDS             3054..4553
                     /gene="pitA"
                     /locus_tag="JNC24_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HAY9159206.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWLSLQFL"
     gene            complement(4797..5132)
                     /gene="uspB"
                     /locus_tag="JNC24_002483"
     CDS             complement(4797..5132)
                     /gene="uspB"
                     /locus_tag="JNC24_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HAY9159207.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            5523..5957
                     /gene="uspA"
                     /locus_tag="JNC24_002484"
     CDS             5523..5957
                     /gene="uspA"
                     /locus_tag="JNC24_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HAY9159208.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            6275..7744
                     /gene="dtpB"
                     /locus_tag="JNC24_002485"
     CDS             6275..7744
                     /gene="dtpB"
                     /locus_tag="JNC24_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
                     /protein_id="HAY9159209.1"
                     /translation="MNTTTPMGMLQQPRPFFMIFFVELWERFGYYGVQGVLAVFFVKQ
                     LGFSQEQAFVTFGAFAALVYGLISIGGYVGDHLLGTKRTIVLGALVLAIGYFMTGMSL
                     LKPDLIFIALGTIAVGNGLFKANPASLLSKCYPPKDPRLDGAFTLFYMSINIGSLIAL
                     SLAPVIADRYGYSVTYNLCGAGLIIALLVYIACRGMVKDIGSEPDFKPMSFSKLLYVL
                     LGSVVMIFVCAWLMHNVEVANLVLIVLSIVVTIIFFRQAFKLDKTGRNKMFVAFVLML
                     EAVVFYILYAQMPTSLNFFAINNVHHEILGFSINPVSFQALNPFWVVLASPILAGIYT
                     HLGNKGKDLSMPMKFTLGMFMCSLGFLTAAAAGMWFADAQGLTSPWFIVLVYLFQSLG
                     ELFISALGLAMIAALVPQHLMGFILGMWFLTQAAAFLLGGYVATFTAVPDNITDPLET
                     LPVYTNVFGKIGLVTLGVAVVMLLMVPWLKRMIATPESH"
     gene            complement(7793..8545)
                     /gene="rsmJ"
                     /locus_tag="JNC24_002486"
     CDS             complement(7793..8545)
                     /gene="rsmJ"
                     /locus_tag="JNC24_002486"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HAY9159210.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(8553..10595)
                     /gene="prlC"
                     /locus_tag="JNC24_002487"
     CDS             complement(8553..10595)
                     /gene="prlC"
                     /locus_tag="JNC24_002487"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HAY9159211.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTQALNDCRENVARVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAGLAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELEQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKSVNGLFEVVKRIYGITAKERKEVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDDCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            10798..11640
                     /locus_tag="JNC24_002488"
     CDS             10798..11640
                     /locus_tag="JNC24_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HAY9159212.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSEFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLPWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            11712..13064
                     /gene="gorA"
                     /locus_tag="JNC24_002489"
     CDS             11712..13064
                     /gene="gorA"
                     /locus_tag="JNC24_002489"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HAY9159213.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(13118..13201)
                     /locus_tag="JNC24_002490"
     CDS             complement(13118..13201)
                     /locus_tag="JNC24_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Damage inducible protein"
                     /protein_id="HAY9159214.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            13229..13402
                     /locus_tag="JNC24_002491"
     CDS             13229..13402
                     /locus_tag="JNC24_002491"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9159215.1"
                     /translation="MHPLTHPLPVTAHVSLLDDDYLTPARASVNGTTRTSDQDFESVY
                     AHYQSENASELTG"
     gene            13941..14294
                     /gene="arsR"
                     /locus_tag="JNC24_002492"
     CDS             13941..14294
                     /gene="arsR"
                     /locus_tag="JNC24_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HAY9159216.1"
                     /translation="MSFLLPIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQ
                     PKISRHLALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKVQAIVRN
                     LARQNCSGDSKNICS"
     gene            14348..15637
                     /gene="arsB"
                     /locus_tag="JNC24_002493"
     CDS             14348..15637
                     /gene="arsB"
                     /locus_tag="JNC24_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HAY9159217.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            15650..16075
                     /gene="arsC"
                     /locus_tag="JNC24_002494"
     CDS             15650..16075
                     /gene="arsC"
                     /locus_tag="JNC24_002494"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HAY9159218.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKVVDEAGKRLK"
     gene            complement(16209..16505)
                     /locus_tag="JNC24_002495"
     CDS             complement(16209..16505)
                     /locus_tag="JNC24_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY9159219.1"
                     /translation="MQLEEVAKALKELGHPTRLFIFKHLVKAGEQGLPVGELQKQLGI
                     PGSTLSHHISALVSVGLVKQNRESRTLMCVSQYAMLEAIIEFLREECCVNSKIA"
     gene            16612..>16873
                     /locus_tag="JNC24_002496"
     CDS             16612..>16873
                     /locus_tag="JNC24_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HAY9159220.1"
                     /translation="MSSWLAMLQDAAEMFVFLAVELSLLFIVISAGVSLIRQKVPDHK
                     IQQMMGARKGRGYLLAALLGAVTPFCSCSTIPMLRGLLSAKAG"
BASE COUNT         3896 a         4380 c         4276 g         4321 t
ORIGIN      
        1 gcaataatac ttatatgttc gatgatttta agcaccgaga ctatccggtc ttttactaaa
       61 taatggaaaa caatccatcc ctggaagata attgtaaaaa aaacaacaga ataagaatta
      121 atcttaggat aaatatttat ttatcatggc cttttaaccg gtaaaaaatg gcactcggca
      181 cagtaaaccc cgctatagtt atgctatgca tttgtcttta atgatatctc gtcgttatat
      241 taacaggctg aaattatcat taatgcacat ttcattatta gcagggatac ctgaggagtt
      301 taaagtgaac atatatatcg ggtggctttg tgctggaaag ttcagggcaa agcgagtatt
      361 tcgtcgcggg tcatgtgctg atttctctgg cggccatatg cctggcatta ttcactaccg
      421 catttattat catttcgcag ctcacgcgcg gcgttaatac gttttacaat acattgttcc
      481 ccattattgg ctatgcgggg tcaattatca ccatgatatg gggttgggca ctgttagcag
      541 gcaatgatgt gatggcagac gagtttgtcg ccggccatgt tattttcggc gttggtatga
      601 ttgccgcctg tgtatcgacg gtggcagcgt catccggtca ctttctgctc attcccaaaa
      661 atgcagcggg gagcaagagc gatggaacgc cggtacaggc ttattcttca ttaatcggta
      721 attgcctcat tgccgttccc gttttactca ccctgctcgg tttcatttgg tctattacgc
      781 tgttacgtag tgctgacata actccgcatt atgtcgcggg tcacgtattg cttgggttaa
      841 ccgcaatctg tgcctgtcta attggccttg ttgccacaat tgtccatcaa acacgtaata
      901 cgttttcaac taaagaacac tggctgtggt gttattgggt tatttttctc ggctcaatca
      961 cggtactgca ggggatatac gtcttagtca gttccgatgc aagcgcccga ctggctcccg
     1021 gcattattct tatttgcctc ggaatgatct gttacagcat attctcaaaa gtctggctac
     1081 tggcactggt ctggagacgt acctgttcgt tagccaacag aataccgatg atacccgtct
     1141 tcacctgcct gttttgcctt ttcctggcat catttcttgc ggaaatggcg cagaccgaca
     1201 tgggatattt tattccttcg cgagttctgg tcggtttggg agcggtatgc tttacgttgt
     1261 tctcaatcgt ttcaatatta gaagcgggtt ctgctaaaaa ataattgcaa cgtaccggat
     1321 aaaaccagcg ttgaccattt gcgtaacgct ggtttttctt aggcatcatg aaataacgca
     1381 gcattaatgc atagtggtta aggataaaaa aacaacaaag ttactgtctt tttcagcctg
     1441 ttcatatttt ataaagatct ggccactggg tgagttttca atcacctttc cttccacctt
     1501 atattaagcc tggagggttt cagttcacgg gctcattaga aaataatcac aagatagcca
     1561 ctatcaaaat tagctcattt ttaatgcggc tctaataatt ttcatcttta ggtaacaggt
     1621 caggacgact tcgctgcaat caaatcctgc gcacaagccc acgcactcga ccacgcccac
     1681 tggaagttat agccccccag ccagccggta acgtccatca cttcgccgat gaagtacagc
     1741 ccaggcactt tgcgcgcttc catcgtccgt gaagagagtt cgttggtgtc cacgccgccg
     1801 agcgtcactt cggcagtgcg atagccttca gtgccgttgg gttgtacacg ccagtcggtc
     1861 aatgtgctaa tcagtgcctg ttggtcacgc acgttgagct gttttagcga aacattcggg
     1921 atttgcccga gttgctgtaa gcgttcaacc aaccgcttcg gtagatgaac cgccagtgtg
     1981 tttttcaggc tctgattcgg atgtgcgtta cgctgctcat tcaggaaggt ttcgagatcc
     2041 acatccggta gcagattgat actgacaaat tcacccggtt gccagtagct ggaaatctgc
     2101 aacaccgccg gtccagacaa gccgcgatgg gtgaagagta agttctcacg gaaaacgatg
     2161 ccgttttcag cggtaatcac ggaaggcacc gccacgcccg ccagcacctg taactcttcg
     2221 agcaacggtt tatgcagagt gaatggcacc agacccgcac gggtcggcag cacgttgagg
     2281 ccaaattgtt cggcaatctt ataaccaaac ggcgacgcgc ccagccccgg cattgacagc
     2341 ccaccggtcg ccatgaccag cttttcgcaa ccgacagtca tgccgttcag atcaagcgtg
     2401 aagcctgttt catcctccgc cacactcagc acttcgctac gcaatctgaa ggtcacattg
     2461 cccttctcgc actcatccac cagcatgtcg acaatctgct gcgcggagtc atcgcagaag
     2521 agttgcccta acgttttctc gtgccatgcg atgccgtgtt tattgaccag atcaatgaaa
     2581 tcccactggg taaaacgtgc gagtgcagac ttacaaaaat gcggattctg gctcagataa
     2641 gcgcctggtt cgacataaag gttggtaaag ttgcagcgcc caccgccaga cataaggatt
     2701 ttgcgccctg gttttttacc attatcgatc agcagaaccc ggcgtcctgc ctgacctgcc
     2761 agcgcagaac agaacatacc cgccgcacca gcgcctataa taatggcatc aaacctttcc
     2821 acgttgcgct cctcttagaa aaaacgggcg tgaattgtaa agattcctca gtggtcgcac
     2881 cagcatcaat attactaaaa ggaagtattt gcctgaatta tataagataa ttattttttg
     2941 agtgaaatcc atacaggagg caaatcaaaa aaagtctata tttcactttg cccgcgccgc
     3001 gaaagtcact gataatgcgc cgcgttcatg tcctcaaaat ggcgtaacgt cctatgctac
     3061 atttgtttgc tggcctggat ttgcataccg ggctgttatt attgcttgca ctggcttttg
     3121 tgctgttcta cgaagccatc aatggtttcc atgacacagc caacgccgtg gcaaccgtta
     3181 tctatacccg cgcgatgcgt tctcagctcg ccgtggttat ggcggcggtg ttcaactttt
     3241 tgggtgtttt gctgggtggt ctgagtgttg cctatgccat tgtgcatatg ctgccgacgg
     3301 atctgctgct taatatggga tcgtctcatg gccttgccat ggtgttctct atgttgctgg
     3361 cggcgattat ctggaacctg ggtacctggt actttggttt acctgcatcc agctctcata
     3421 cgctgattgg cgcgatcatc gggattggtt taaccaatgc gttgatgacc gggacgtcag
     3481 tggtggatgc actcaatatc ccgaaagtat taagtatttt cggttctctg atcgtttccc
     3541 ctattgtcgg cctggtgttt gctggcggtc tgattttctt gctgcgtcgc tactggagcg
     3601 gcaccaagaa acgcgcccgt atccacctga ccccagcgga gcgtgaaaag aaagacggca
     3661 agaaaaagcc gccgttctgg acacgtatcg cgctgatcct ttccgctatc ggcgtggcgt
     3721 tttcgcacgg cgcgaacgat ggtcagaaag gcattggtct ggttatgttg gtattgattg
     3781 gcgtcgcgcc agcaggcttc gtggtgaaca tgaatgccac tggctacgaa atcacccgta
     3841 cccgtgatgc catcaacaac gtcgaagctt actttgagca gcatcctgcg ctgctcaaac
     3901 aggctaccgg tgctgatcag ttagtaccgg ctccggaagc tggcgcaacg caacctgcgg
     3961 agttccactg ccatccgtcg aataccatta acgcgctcaa ccgcctgaaa ggtatgttga
     4021 ccaccgatgt ggaaagctac gacaagctgt cgcttgatca acgtagccag atgcgccgca
     4081 ttatgctgtg cgtttctgac actatcgaca aagtggtgaa gatgcctggc gtgagtgctg
     4141 acgatcagcg cctgttgaag aaactgaagt ccgacatgct tagcaccatc gagtatgcac
     4201 cggtgtggat catcatggcg gtcgcgctgg cgttaggtat cggtacgatg attggctggc
     4261 gccgtgtggc aacgactatc ggtgagaaaa tcggtaagaa aggcatgacc tacgctcagg
     4321 ggatgtctgc ccagatgacg gcggcagtgt ctatcggcct ggcgagttat accgggatgc
     4381 cggtttccac tactcacgta ctctcctctt ctgtcgcggg gacgatggtg gtagatggcg
     4441 gcggcttaca gcgtaaaacc gtaaccagta ttctgatggc ctgggtgttt acccttccgg
     4501 ctgcggtact gctttccggc gggctgtact ggctctcctt gcagttcctg taatcgtacg
     4561 caccaaaacg agcgggtcag ctggcccgct tcagattgtg acatagtgcg ctttgttcat
     4621 gccggatgcg gcgtgaacgc cttatccggc ctacaaaaac atgcaaattc aatagattgc
     4681 agagattatg taggcctgat aagcgtagcg catcaggcaa ttttgcgttt gtaatcagtc
     4741 tcgagcgggt cagtttactg gcccgctttt tttatgcgtt attattgctg aagtgcttaa
     4801 tgccaaatca tcaatgcaat caggctgacc accaccagac cacacaatgc gctggtcaga
     4861 ataaactgcc gacgcacccg ctcacagcgg cgaataaatt catcatcgtg atgatcgcga
     4921 taacgttggg catagatata ccaaacgaga cgcacctgtt tgttgggttg gccatgtgag
     4981 gtaaaaaagc cccctccatc aacatattga tagagcaatg gatcgcagtt acgcagtacc
     5041 actaacaacg cgcgtagtga tgagaaatag cgcgccatgt taacaatgca aacgacacat
     5101 aaagcccaaa ataatgcgac ggtgcttatc atacctcctc cccggcgacc tgcccgcgga
     5161 gttccacccc ggggctaccg ctcccgatac gctgccaatc agttaacacc aggtcctgga
     5221 gaaaccgctt ttgtggtgac caacatccga gcggctctat agatagtgta ggagatcagg
     5281 ttgttttttt tccagaaggt taaccactat caatatattc atgtcgaaaa tttgtttatc
     5341 taacgggtaa gcaaggcgga ttgacggatc atccgggtcg ctataaggta aggatggtct
     5401 taacactgaa tctttacggc tgggttagcc ccgcgcacgt agttcgcagg acgcgggtga
     5461 cgtaacggca caagaaacgc tagctggcca gtcatcgaca actttatgga aggagtaaca
     5521 ctatggctta taaacacatt ctcatcgcgg tcgatctctc cccggaaagc aaagttctgg
     5581 tagagaaagc agtctctatg gctcgcccct acaatgcgaa agtttctctg atccacgtag
     5641 atgtaaacta ctctgaccta tacaccgggc ttattgatgt gaatctgggt gatatgcaga
     5701 aacgcatctc tgaagagaca catcatgcgc tgaccgagct ttccactaat gcaggctacc
     5761 caatcactga aaccctgagc ggcagcggcg acctgggcca ggttctggtc gatgcaatca
     5821 agaaatacga tatggatctg gtggtttgtg gtcaccacca ggacttctgg agcaaactaa
     5881 tgtcttccgc acgtcagctg atcaacaccg ttcacgttga tatgctgatt gttccgctgc
     5941 gcgacgaaga agaataatca tccccccacg acgtgttcct gaacgcccgc atatgcgggc
     6001 gttttgcttt ttggcgcgcc ttgttacctg atgcagcgta aaccccttat ctggcctacg
     6061 atctgcgtac gcaatcaaaa tctcctgcca atacaacatt taacactatc atattttcca
     6121 tcattagtgt gatcagctgg ttattttctg ttgtaatagt gtattaatct attcaccgca
     6181 tcaatattaa gaatctctga cagatgtaaa ctttttcgcg cgttatccct tacgcgttca
     6241 tacttttcag gatggtattg gaaggttaat aaatatgaat acaacaacac ccatggggat
     6301 gctgcagcaa cctcgcccat ttttcatgat cttttttgtc gagttatggg agcgattcgg
     6361 ctactacggc gtgcagggcg tactggcggt tttcttcgtt aaacagcttg gattctcgca
     6421 agagcaggct tttgtcactt ttggtgcttt tgctgcgctg gtctatggcc tcatttccat
     6481 tggcggctat gtcggcgacc acctgctggg gaccaaacgc accattgttc tcggagcact
     6541 tgtgctggcg attggctact tcatgaccgg catgtcgcta cttaagcctg acctgatttt
     6601 catcgccctg gggactatcg ctgtcggtaa cggcctgttt aaagctaacc cagccagctt
     6661 gctttcgaag tgctatccgc cgaaagatcc gcggcttgat ggcgcattca ccctgttcta
     6721 tatgtcgatc aatatcggct cgttgatagc gttatcgctg gcccctgtga tcgctgatag
     6781 atacggttat tcagtcacct acaacctgtg cggggcgggg ttaattatcg cattactggt
     6841 ttacatcgcc tgtcgtggaa tggtgaaaga cattggttct gaacccgact tcaagccgat
     6901 gagcttcagc aaactgttgt acgtattact tggcagcgtg gtgatgatct tcgtatgtgc
     6961 atggctgatg cacaacgtag aagtcgccaa tctggtgctg attgttctct ccatcgtcgt
     7021 caccatcatc ttctttcgtc aggcattcaa gctggataaa accgggcgca ataaaatgtt
     7081 tgtcgccttt gtcctgatgc tcgaagcggt ggtgttttac attctctacg cccagatgcc
     7141 aacgtcgctg aacttctttg ccatcaacaa cgtgcatcat gaaattctcg gcttttccat
     7201 aaacccggtc agcttccagg cgcttaaccc gttctgggtg gtactcgcca gcccaatact
     7261 ggcaggcatt tacacgcatc tgggtaacaa aggcaaagac ctctcgatgc cgatgaaatt
     7321 tactctcggc atgtttatgt gctcactggg ctttttgacg gcggcagctg cgggaatgtg
     7381 gtttgcggat gcacaagggc tgacatcgcc atggtttatc gtgctggtgt atttattcca
     7441 gagcctgggt gaattgttta ttagcgccct tggcctggcg atgattgctg ccctggtgcc
     7501 gcagcatttg atgggcttta ttctcgggat gtggttcctg acgcaggctg ccgcattctt
     7561 gctgggcggc tatgtggcaa catttaccgc agtgccggac aacattaccg atccgcttga
     7621 gacgttaccc gtctatacca acgtgtttgg taagattggc ctggttacgc tgggcgttgc
     7681 agtagtgatg ctgttgatgg tgccgtggct gaaacgcatg attgcgacgc cggaaagcca
     7741 ttaattattc ttgcagaaag cagggtagcg ttatcgctac cctgctttac ttttacacgg
     7801 gcgtacctgc atagatatca aagcgatgcc ctttagtcac aaccgcgttt ggcgtggcga
     7861 cattcgctag cggtggcgcg tagtccgggc gctttactac cacgcgtttg gtcgccagca
     7921 agcgtgcagg ctccagcaat ccatcggcat caagatccgg ccccaccagc gactgaaaga
     7981 cacgcatctc ttttttcacc agcgcgcttt tctgcttatg cgggaacatc gggtcgagat
     8041 agaccacctg cgggcgtggg gtaatatcgg ttagcgccgt caggctggag gcgtgaatta
     8101 actgcaaacg ctcctgcaac cagccgccga tttccgcatc cgcataacca cgcgccaggc
     8161 cgtcgtcgag cagcgcggca acaactggat tacgctccag catccgcacg cggcaaccga
     8221 ctgaagccag cacaaaggca tcgcgcccca gtcctgcagt ggcatccacc acatccggca
     8281 aataatcgcc tttaatgccc accgctttcg ccaccgcctc accgcgacca ccgccgaatt
     8341 tgcgtcggtg cgccatcgct ccgccaacaa aatcaacaaa gatgccgcca agttttggct
     8401 catcacgctt gcgcaattcc agatgttccg gcgttaacac cagcgccatc aggttgtctt
     8461 catcgtgctc cagcccccag cgggccgcca gaacagataa ggcgccgtct ccggcgcctg
     8521 tttcatcaat taagcagatt ttcactgaat gatcagccct taatgccgta atgctccagc
     8581 atcgcatcca gctgcggttc acgaccacgg aagcgtttga acagatccat cggctcttct
     8641 gaaccgccac ggctcagaat gttgtcgagg aacgactgcc cggtttcacg gttgaaaatg
     8701 ccctcttcct caaagcgcga gaaagcatct gccgccagta cgtcagccca caggtagctg
     8761 tagtaacctg ccgcataacc accggcgaaa atatggctga aagcgtgcgg gaaacggccc
     8821 caggacggag atggcaccac ggcaaccagt ttcttgattt ctgccagagt ttcgaggatt
     8881 tttgccccct gatccgggcg gaactcggca tgaaggcgga aatcaaacag gccgaactcc
     8941 agctgacgca gaataaacag cgccgcctgg tagttcttcg ccgccagcat tttatccagc
     9001 aactctttcg gcagcggttc gccggtttca tagtgaccag agataaacgc cagcgcctcc
     9061 ggctcccagc accagttttc cataaactga ctcggcagtt cgaccgcatc ccacggcaca
     9121 ccgctgatac cggaaacacc agcggtttcg atgcgggtca gcatatggtg caggccgtga
     9181 ccgaactcgt ggaacagggt gatcacttcg tcgtgagtga acagcgccgg tttaccattt
     9241 accgggcggt tgaagttaca agtcaaatac gcgaccggtt tttgcagaga accatcagct
     9301 ttacgcatct ggcctacgca gtcatccatc cacgccccgc cgcgcttgtt ttcacgggca
     9361 tacagatcga ggtagaagct gccgcgcagt tcgttatttt cgtcatacag ttcgaagaaa
     9421 cgtacatccg gatgccagac atcaacctct ttacgctctt tagcggtgat gccgtaaata
     9481 cgtttcacca cttcaaacag gccgttaacc gatttgtttt ccgggaagta cggacgcagc
     9541 tgctcgtcgc tgatgctgta gaggtgctgt ttctgttttt cgctgtagta agcgatatcc
     9601 cacggctgca actcatcgac accaaattcg gctttagcga aggcacgcag ttgctccagc
     9661 tctttttcgc cttgcggacg cgcgcgtttt gccagatcgg ttaagaaatc cagcacctgc
     9721 tgcgggtttt ctgccatttt agtggcaagg gatttaaagg cgtagttttc aaagcccagc
     9781 agttgcgcca gttcgtgacg cagcgcgagg atctcttcca tcaccttgct gttatcccac
     9841 ttaccggcgt tcgggccttg atcggaggcg cgggtgctgt aagcgcgata catctcttcg
     9901 cgcagagcct ggttgtcgca gtaggtcatt accggcaggt agcttgggat atccagcgtc
     9961 agcaggtagc cttccagctc tttcgcttcg gcctgggctt ttgccgcagc cagcgcgctt
    10021 tctggcatcc ccgccagccc cgcttcgtcg gtaacgagtt tggtccagcc catcgtcgca
    10081 tcgaggacgt tgttgctgta ctggttgccc agttcagaaa gacgggtagc aatttcgccg
    10141 taacgctgct gtttctcttt cggcagacct atgccagaga gttcaaaatc acgcagggcg
    10201 ttatcaaccg ctttcttctg cgccgtgttc agcgtggcgt aatgatcgcc atcgcgcagg
    10261 tcgcggtacg ctttatacag cccttcatgt tgccctaccc aggtgctgta ttccgacagc
    10321 agcggcaggg tttgctcgta ggcttcacgc agttccgggc tatttttcac cgagttcagg
    10381 tggctgaccg gggagaagat acgccccaac acatcgtcca cttccgccaa cggctggcag
    10441 agattttccc aggtgtacgg tgccccttgc gctactacgc gcgccacatt ttcgcggcaa
    10501 tcgttcagcg cctgagtcac ggctggaacg acatgttccg ggagaatttt agaaaacgga
    10561 ggcaattcaa agggagtcag taacggattc gtcattcgcg cagtcctggt taaagaggtt
    10621 aaggaagcgc tcaacaggcg ctttacataa tgtgtgtagc atggggttaa gtgtagtgaa
    10681 tttcaatgag aaacgttacg ctttcgcggc ggcggcctct tttcggtata ctgtcctgat
    10741 acgcttttgt gcgccccgaa tacgggccga tttttactta ccggaacacc tttacccatg
    10801 ctcagttatc gccacagctt tcacgctggc aaccacgccg acgtccttaa acataccgtt
    10861 cagagcctga tcatcgagtc gctgaaagag aaagataaac cgtttctcta tctcgatacc
    10921 cacgcagggg ccgggcgtta tcagttaggc agcgaacatg ccgagcgtac cggcgaatat
    10981 ctcgaaggta tcgcccgtat ctggcagcag gacgatttgc ccgcagaact ggaggcgtac
    11041 atcaatgtgg taaaacactt caaccgtagc ggtcagttgc gttactaccc cgggtcgccg
    11101 ttgattgctc gccagctact gcgcgaacaa gacagcctgc aactgaccga actgcacccg
    11161 agcgattacc cgttgctgcg ttctgaattt cagaaagaca gccgtgcgcg cgtcgaaaaa
    11221 gccgacggtt tccagcagct taaggccaaa cttccgccgg tttcccgccg tggtttaatc
    11281 cttatcgacc cgccgtatga aatgaaaacc gactatcagg cggtggtcag cgggatagca
    11341 gaaggttaca aacgtttcgc cactggtact tacgcactgt ggtatccggt ggtgctgcgt
    11401 cagcaaatta agcgcatgat ccacgatctg gaagcgaccg gtattcgcaa aattctgcaa
    11461 attgaactgg cggtactgcc agacagcgat cgccgtggca tgaccgcttc cggcatgatt
    11521 gtgattaacc cgccgtggaa actggaacag cagatgaata acgtgctgcc gtggctgcac
    11581 agcaaactgg ttccggcagg caccgggcac gccaccgtaa gctggatcgt gccggagtaa
    11641 ttgcagccat tgctggcacc tattacgtct cgcgctacaa tcgcggtaat caacgataag
    11701 gacactctgt catgactaaa cactatgatt acatcgccat cggcggcggc agcggcggta
    11761 tcgcctccat caaccgcgcg gctatgtacg gccagaaatg cgcactgatt gaagccaaag
    11821 agctgggcgg cacctgcgtg aacgtcggct gcgtgccgaa aaaagtgatg tggcacgcgg
    11881 cgcaaatccg tgaagcgatc catatgtacg gccctgatta tggtttcgat accaccatca
    11941 acaagttcaa ctgggaaacg ttgatcgcca gccgtaccgc ctatatcgac cgtattcata
    12001 cttcctatga aaacgtgctc ggtaaaaata acgttgatgt aatcaaaggc tttgcccgct
    12061 tcgttgatgc caaaacgctg gaggtaaacg gcgaaaccat cacggccgat catattctga
    12121 tcgccacagg cggtcgtccg agccacccgg atattccggg cgtggaatac ggtattgatt
    12181 ctgatggctt cttcgccctt cctgctttgc cagagcgcgt ggcggttgtt ggcgcgggtt
    12241 acatcgccgt tgagctggcg ggcgtgatta acggcctcgg cgcgaaaacg catctgtttg
    12301 tgcgtaaaca tgcgccgctg cgcagcttcg acccgatgat ttccgaaacg ctggtcgaag
    12361 tgatgaacgc cgaaggcccg cagctgcaca ccaacgccat cccgaaagcg gtagtgaaaa
    12421 atgccgatgg tagcctgacg ctggagctgg aagatggtcg cagtgaaacg gtggattgcc
    12481 tgatttgggc gattggtcgc gagcctgcca atgacaacat caacctagaa gccgctggcg
    12541 ttaaaactaa cgaaaaaggc tatatcgtcg tcgataaata tcaaaacacc aatattgaag
    12601 gtatttacgc ggtgggcgat aacacgggtg cagtggaact gacaccggta gcagttgcag
    12661 cgggtcgccg tctctctgaa cgcctgttta ataacaagcc ggatgagcat ctggattaca
    12721 gcaacattcc gaccgtggtc ttcagccatc cgccgattgg tactgttggt ttaacggaac
    12781 cgcaggcgcg cgagcagtac ggcgacgatc aggtgaaagt gtataaatcc tctttcaccg
    12841 cgatgtatac cgccgtcacc actcaccgcc agccgtgccg catgaagctg gtgtgcgttg
    12901 ggccagaaga gaagattgtc ggtattcacg gcattggctt tggtatggac gaaatgttgc
    12961 agggcttcgc ggtggcgctg aagatgggcg caaccaaaaa agacttcgac aataccgtcg
    13021 ccattcaccc aacggcggca gaagagttcg tgaccatgcg ctaaatgtta aagggctaag
    13081 agtagtgtgc tcttagccct taattacgtt tccgctatca gttcagaagc tgaagcagaa
    13141 agcggatcag ttccagcagc gcaattaacg ctccaagaac gatgattgct ttatcaatca
    13201 cccgttttct ccatgcgatg gagtgagaat gcatccgctt actcatccac tgcctgtcac
    13261 ggcgcatgtc tcattgttag atgacgacta tctcactccg gccagagcat cagttaacgg
    13321 caccacccgt acttctgacc aggactttga aagcgtttat gcgcattacc agagtgaaaa
    13381 tgcctcagag ctaactggat aatcatacag tacattcagg ttataaaacc agcatgtcct
    13441 tgcaatagtt tcagtatggt attagcattg atgcgttaga tgatgactat ctcactccag
    13501 ccagagccac caactcaggg ctgaagagta aaaaaaccga cgcaaagtcg gtttttttac
    13561 atccggattc ggacaaggct taatatgacg atgacccagt gaaagtatat aaatcgtcat
    13621 tgcgatatat accgaagtgc cccctccgcc agctgaagaa atcgctaatt cttgcaatgt
    13681 tagccactgg ctaatagtat tgaaccgtta gatgtgggca ttctcactcc agccagagcc
    13741 accaactcag gctgaagagt aaaaaaccga cgcaaagtcg gtttttttac gtcctgattc
    13801 aaaccgcctt tcaaatgaat agccaactca aaattcacac ctattacctt cctctgcact
    13861 tacacattcg ttaagtcata tatgtttttg acttatccgc ttcgaagaga gacactacct
    13921 gccacaatca ggagcgcaat atgtcatttc tgttacccat ccaattgttc aaaattcttg
    13981 ctgatgaaac ccgtctgggc atcgttttac tgctcagcga gctgggagag ttatgcgtct
    14041 gcgatctttg tactgccctc gatcagtcgc agcccaagat ctcccgccac ctggcattgc
    14101 ttcgtgaaag cgggctattg ctggaccgca agcaaggtaa gtgggttcat taccgcttat
    14161 caccgcatat tccagcatgg gcggcgaaaa ttattgatga ggcctggcga tgtgaacagg
    14221 aaaaggttca ggcgattgtc cgcaacctgg ctcgacaaaa ctgttccggg gacagtaaga
    14281 acatttgcag ttaaaaattt agctaaacac atatgaattt tcagatgtgt tttatccggg
    14341 aggcattatg ttactggcag gcgctatctt tgtcctgacc atcgtattgg ttatctggca
    14401 gccgaaaggt ttaggcatcg gctggagtgc aacgctcggc gcagtactgg cgttagttac
    14461 gggcgtggtt catccgggtg atattccggt ggtgtggaat atcgtctgga acgcgacggc
    14521 tgcgtttatc gccgtcatta tcatcagcct gctgctggat gagtccggct tttttgaatg
    14581 ggcggcgctg cacgtctcac gctggggtaa tggtcgtggt cgcttgctgt ttacctggat
    14641 tgtcctgctc ggtgctgccg ttgccgccct gtttgccaat gatggcgcgg cgcttatttt
    14701 gacgccgatt gtcatcgcca tgctgctggc tttaggattc agtaaaggca cgacgctggc
    14761 gttcgtgatg gcggccggat tcattgccga taccgccagc ctgccgctta ttgtctccaa
    14821 cctggtgaat atcgtttccg ctgatttctt tggcctcggc tttcgcgaat acgcctcggt
    14881 gatggtgccg gtggatatcg ccgcgattgt tgccacgctg gtgatgctac atctctattt
    14941 tcgcaaagat attccgcaga actacgatat ggcgctgctg aaatctcccg cagaagcgat
    15001 caaagatcct gctacgttca aaactggctg ggttgtttta ctgcttctgc tggtgggatt
    15061 tttcgtcctg gaaccgctcg gcattccggt gagcgccatt gcagctgtgg gcgcgctgat
    15121 attatttgtc gtcgctaaac gcggtcatgc gattaatacg ggtaaagtcc tgcgcggtgc
    15181 cccctggcag attgtcatct tctcgctcgg catgtatctg gtggtttatg gcctgcgcaa
    15241 tgccggatta acggaatatc tttctggcgt actcaacgtg ctggcggata acggcctgtg
    15301 ggccgcgacg ctcggcaccg gattcctcac cgccttcctc tcttctatta tgaacaatat
    15361 gccgacggta ctggttggcg cgttgtccat tgatggcagc acggcatctg gcgttatcaa
    15421 agaagcgatg gtttatgcca atgtgattgg ctgcgatttg ggaccgaaaa ttaccccaat
    15481 tggtagcctg gctacgctac tctggctgca cgtactttcg cagaagaata tgactatcag
    15541 ctggggatat tacttccgta cagggattat catgaccctg cctgtgctgt ttgtgacgct
    15601 ggctgcgctg gcgctacgtc tctctttcac tttgtaatga gatactgata tgagcaacat
    15661 taccatttat cacaacccgg cctgcggcac gtcgcgtaat acgctggaga tgatccgcaa
    15721 cagcggcaca gaaccgacca ttatccatta tctggaaact ccgccaacgc gcgatgaact
    15781 ggtcaaactc attgccgata tggggatttc cgtacgcgcg ctgctgcgta aaaacgtcga
    15841 accgtatgag gagctgggcc ttgcggaaga taaatttact gacgatcggt taatcgactt
    15901 tatgcttcag cacccgattc tgattaatcg cccgattgtg gtgacgccgc tgggaactcg
    15961 cctgtgccgc ccttcagaag tggtgctgga aattctgcca gatgcgcaaa aaggcgcatt
    16021 ctccaaggaa gatggcgaga aagtggttga tgaagcgggt aagcgcctga aataaagcgg
    16081 cgatatccac tgccgatagt tggtaaagtg cgctttgttt atgtcggatg cgacgctaac
    16141 gcgtcttatc cgacctacaa aaccgtgcaa attcaatata tggcatgaaa tatgtagagc
    16201 gtagcgcatc aggcaatttt gctatttacg cagcactcct cgcgtaagaa ttcaattatc
    16261 gcttccagca ttgcgtattg cgaaacacac atcagcgtcc gactctcacg gttttgtttg
    16321 accagtccta ccgataccag tgctgatata tggtggctga gtgtggagcc aggtatccca
    16381 agctgctttt gcaactcacc gaccggcaac ccttgttccc cggctttgac aagatgttta
    16441 aatatgaaca aacgggtcgg gtgacctagc tctttcagcg ctttagcgac ttcttccaac
    16501 tgcatatgac acccctctgt ctggttgata tttcgataat accagaaata tcttgatctg
    16561 aatttcattt cgattattct ggaaatatag atttatccaa tgaggttacg tatgagtagt
    16621 tggcttgcga tgctgcaaga tgccgcagag atgtttgtgt ttctcgccgt cgagctttcg
    16681 ttgctgttta tagtgattag tgccggtgtc agcctgataa gacaaaaggt gccagaccat
    16741 aaaatccagc agatgatggg ggccagaaaa gggagaggct atctcctggc tgctctgttg
    16801 ggagccgtta ccccgttctg tagttgctcg acaataccca tgttacgtgg attgttgtca
    16861 gcgaaagccg ggt
//
LOCUS       DADMKF010000082        16742 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.083, whole genome shotgun sequence.
ACCESSION   DADMKF010000082 DADMKF010000000
VERSION     DADMKF010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16742
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.083"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            78..2393
                     /locus_tag="JNC24_002497"
     CDS             78..2393
                     /locus_tag="JNC24_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin guanine dinucleotide-containing
                     S/N-oxide reductase"
                     /protein_id="HAY9159221.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALDLIHQQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLARCTTGYA
                     VFASYLLGESDGIAKTAEWAAEICGVGAAKIRQLAAIFHQNTTMLMAGWGMQRQQFGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFTRRSAVLSSMQGSLPGGCDAVDKIPVARI
                     VEALENPGGAYQHNGMNRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPELVVISEC
                     FWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDFDVFAEL
                     SERWEKGGYARFTEGKSQLQWLETFYNVARQRGASQQVELPPFAEFWQANQLIEMPEN
                     PDSERFIRFADFCRDPLAHPLKTASGKIEIFSQRIADYGYPDCPGHPMWLEPDEWQGN
                     AEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGITEGDMVRV
                     WNSRGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDLPSSRLG
                     NGCAGNTALAWLEKYNGPELTLTAFEPPASS"
     gene            complement(2362..2802)
                     /locus_tag="JNC24_002498"
     CDS             complement(2362..2802)
                     /locus_tag="JNC24_002498"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAY9159222.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLML
                     EVYQKNQPAIDFYRAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(2799..3362)
                     /gene="tag"
                     /locus_tag="JNC24_002499"
     CDS             complement(2799..3362)
                     /gene="tag"
                     /locus_tag="JNC24_002499"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HAY9159223.1"
                     /translation="MERCGWVSQDPLYIAYHDNEWGVPETDSKKLFEMICLEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFADFVWSFVNHQPQVTQATTLSEIPTSTSASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYPGNKP"
     gene            3520..4218
                     /locus_tag="JNC24_002500"
     CDS             3520..4218
                     /locus_tag="JNC24_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HAY9159224.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPNYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTQTDPLWHASVSTLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALTQAENTTN
                     NSDYLYTMGVSARF"
     gene            4447..5655
                     /locus_tag="JNC24_002501"
     CDS             4447..5655
                     /locus_tag="JNC24_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY9159225.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFCVKRVTMASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSIFGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHGSL"
     gene            5979..7670
                     /gene="eptB"
                     /locus_tag="JNC24_002502"
     CDS             5979..7670
                     /gene="eptB"
                     /locus_tag="JNC24_002502"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HAY9159226.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LAVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDETYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKEAAAN"
     gene            7762..7838
                     /locus_tag="JNC24_002503"
     tRNA            7762..7838
                     /locus_tag="JNC24_002503"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7796..7798,aa:Pro,seq:cgg)
     gene            7873..8006
                     /locus_tag="JNC24_002504"
     ncRNA           7873..8006
                     /ncRNA_class="other"
                     /locus_tag="JNC24_002504"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            8751..10358
                     /gene="dppA"
                     /locus_tag="JNC24_002505"
     CDS             8751..10358
                     /gene="dppA"
                     /locus_tag="JNC24_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HAY9159227.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTRELNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQTPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            10666..11685
                     /gene="dppB"
                     /locus_tag="JNC24_002506"
     CDS             10666..11685
                     /gene="dppB"
                     /locus_tag="JNC24_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HAY9159228.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHAQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            11695..12597
                     /gene="dppC"
                     /locus_tag="JNC24_002507"
     CDS             11695..12597
                     /gene="dppC"
                     /locus_tag="JNC24_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HAY9159229.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYIVIVLF
                     ITIFANWIAPYNPADQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            12608..13591
                     /gene="dppD"
                     /locus_tag="JNC24_002508"
     CDS             12608..13591
                     /gene="dppD"
                     /locus_tag="JNC24_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HAY9159230.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLVFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            13588..14592
                     /gene="dppF"
                     /locus_tag="JNC24_002509"
     CDS             13588..14592
                     /gene="dppF"
                     /locus_tag="JNC24_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP binding subunit
                     DppF"
                     /protein_id="HAY9159231.1"
                     /translation="MSTQEATLQQPLLQAIDLKKHYPVKKGMFAPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLSGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(14622..15893)
                     /locus_tag="JNC24_002510"
     CDS             complement(14622..15893)
                     /locus_tag="JNC24_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HAY9159232.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLVTIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRVLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
     gene            16369..16476
                     /locus_tag="JNC24_002511"
     CDS             16369..16476
                     /locus_tag="JNC24_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HAY9159233.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
BASE COUNT         3986 a         4161 c         4521 g         4074 t
ORIGIN      
        1 ttattcctgg gaatactcgg aaatttgtaa gtaatattta actgctcaat acatctaacc
       61 tttcaggagt cttcggtttg gccaactcat cctcacgata ttccgttctg actgccgccc
      121 actgggggcc catgctggtt gaaaccgacg gcgaaaccgt gtttagctcg cgtggcgcgt
      181 tagccacagg aatggaaaac tccttgcaga gcgcggttcg cgaccaggtt cacagcaata
      241 cgcgggtacg atttccaatg gtgcgcaaag gctttctcgc gtcaccggaa aacccgcaag
      301 gcattcgtgg gcaggatgaa tttgttcgcg tgagttggga tgaggcgctg gatcttattc
      361 accaacaaca taaacgcatt cgtgaggctt atggtccggc atcgattttt gctggttcct
      421 acggctggcg ttcaaacggc gtgctgcata aggcctcgac attattacaa cgctatatgg
      481 cgctggcagg cggttatacc gggcatctgg gggattattc gaccggtgcg gcacaggcga
      541 tcatgccgta tgtcgtaggt ggcagcgaag tttatcaaca gcaaaccagt tggccgctgg
      601 tgctggaaca tagcgatgtc gtggtgttgt ggagtgctaa cccactcaat acgctgaaaa
      661 ttgcgtggaa tgcatccgat gagcaggggc tttcttactt ttccgcactg cgtgacagtg
      721 ggaaaaagct gatctgcatt gatccaatgc gatcggaaac cgtcgatttc tttggcgata
      781 aaatggagtg ggtggcaccg cacatgggca ccgatgttgc gctgatgctg gggatcgccc
      841 atacgctggt ggaaaatggt tggcacgacg aagcgtttct ggcgcgttgc accacaggtt
      901 atgccgtctt cgcctcttat ttgctgggcg agagtgacgg aatagcgaaa accgccgaat
      961 gggcagcaga gatttgtggt gttggcgcag cgaaaatccg ccagctggcg gctattttcc
     1021 accaaaatac caccatgctg atggcaggct ggggaatgca gcgccaacag tttggtgagc
     1081 aaaaacactg gatgatcgtc acgctggcag caatgttggg gcaaatcggc acacccggcg
     1141 gcggttttgg tctttcttac cattttacgc ggcgttctgc ggtgctctct tccatgcagg
     1201 gcagcttgcc gggtggctgc gatgcggtgg ataaaatccc tgttgcccgc attgttgaag
     1261 cactggaaaa ccctggtggc gcatatcaac acaacggtat gaaccgacat ttcccggata
     1321 ttcgttttat ctggtgggcg ggcggtgcca actttactca tcatcaggat accaatcgtc
     1381 tgatccgtgc ctggcaaaaa ccggagctgg tggtgatctc tgaatgcttc tggacggcgg
     1441 cggcgaaaca cgcggatatc gttctgcctg cgactacctc ttttgagcgt aatgatctca
     1501 ccatgaccgg tgattacagt aatcagcatc tggtgccgat gaagcaagtg gtgccgccac
     1561 gctatgaagc gcgtaatgat tttgatgttt ttgccgagtt gagtgaacgt tgggagaagg
     1621 gcggctatgc gcggtttacg gaaggaaaaa gtcagctgca atggctggaa acgttttata
     1681 acgttgcccg gcagcgcggg gcaagccagc aggttgaatt gccgccattt gctgagttct
     1741 ggcaagccaa ccagttaatt gagatgccgg aaaacccgga cagcgagcgg tttattcgct
     1801 tcgctgattt ttgccgcgat ccgctggcgc atccgttaaa aaccgccagc ggcaagattg
     1861 aaatcttctc acagcgtatt gccgattacg gttacccgga ttgccctggg catccaatgt
     1921 ggctggagcc ggacgaatgg cagggcaatg ccgaaccaga acagttgcag gtactttctg
     1981 cccatccggc gcaccgcctg cacagccagc tgaattacag ttctctgcgc gaattgtacg
     2041 cggtagcaaa ccgtgagcct gtcaccattc atcctgacga tgctcaggcg cgcggcataa
     2101 ccgaagggga tatggtgcgg gtgtggaact cccgtgggca gatccttgcc ggagcggtca
     2161 ttagcgaggg aattaaacct ggcgtgattt gcattcacga aggggcatgg ccggatctgg
     2221 atttaaccgc tgacggtatt tgtaaaaacg gcgcagtgaa cgtgctgacc aaagatctcc
     2281 ccagctcgcg gctggggaat ggctgtgcgg gtaatacggc gctggcatgg ctggaaaaat
     2341 acaacggtcc ggaactgaca cttacagcgt ttgaaccacc ggccagctca taatccaggt
     2401 gggtagttgg gtttcatcct gccatgcgca atcgacaatg tgaaaaccct gcgcacggta
     2461 aaaatctatc gccggttgat ttttttgata aacctccagc atcagatggg gatagcgctg
     2521 ctgcacatac tgcatcagcg ccttaccaat accgcgtctg acggccttcg gtgcgacaaa
     2581 cattgctgcc agaaatcggc cttccataat gctgacaaaa ccgagaagct taccgtcctc
     2641 ttcccagacc cagttttgcg cgttggcaag ataggcatcc cgcaccagcg gaatgcagtc
     2701 acgccagtaa ttcgctttta taaagggatg cccccaggtt gtactttcca gccacagttc
     2761 gaggatcgcg gggagttctg aacgttgcgc ttcccgaatc atggtttatt tcccggatag
     2821 caacaacagc caaccacatg atcattcacc agcccacatg cctgcataaa ggagtaacag
     2881 attgtggtgc cgacaaactt aaaaccacgt tttttcagtg ctttagatag ggcgtcggag
     2941 gcggacgtag atgtgggaat ttcgctcaac gttgtggctt gtgtcacctg tggctgatga
     3001 tttacaaacg accagacaaa gtcggcaaac ggttcgccgt tctgttccat ttgcaggtac
     3061 gcccgcgcat taccaataat tgcctgaatt ttccctcgat ggcggataat cccggcgtcc
     3121 tgtaccagtc tttcgacatc ctcttcctgc attgctgcga ccttcaccgg atcgaactga
     3181 tgaaagcagg cgcgatagtt ttcgcgtttt ttgaggacgg tgatccacga taacccagcc
     3241 tgctgccctt caaggcagat catttcgaac agttttttac tgtcagtttc aggcacgccc
     3301 cattcattat catggtaggc aatataaagc gggtcctgac tcacccagcc gcaacgttcc
     3361 atactttccc tcgctatgta cccgatcgta aaaatttcag ccgcttttct tgacgcatcg
     3421 gtgaaaaagt caatatttaa tacagggcta ctatgcccga tatccttcat acaatgacaa
     3481 taatattgcc gaattcggct cttccctgga gtcgtgttaa tgatcataaa aaaaagcggt
     3541 ggtcgctggc agctaagcct gctggcgagc gtggtaatca gtgccttttt tctcaacaca
     3601 gcttacgcct ggcaacaaga atatatcgtt gatacgcaac ccggacattc cacagagcgt
     3661 tacacctggg atagtgatca tcaacctaat tacaacgata ttttgtcgca acgtattcaa
     3721 agtagccaaa gggcgctggg actggaagtc aatctggcgg aagaaactcc tgtggatgtg
     3781 accagcagta tgagtatggg ctggaatttt cctttatatg aacaggttac aaccggcccg
     3841 gtcgcggcat tacattacga tggcacaacc acctcgatgt ataacgagtt tggcgacagt
     3901 actaccacgc agaccgatcc gttatggcat gccagcgtga gtaccttagg ctggcgtgtt
     3961 gactcccggc ttggcgatct ccgaccctgg gcgcaaatca gctataacca gcaatttggc
     4021 gagaatatct ggaaggcgca atcaggcctg agccggatga cggcgacaaa ccagaacggc
     4081 aactggctgg atgtcaccgt aggcgctgat atgttgctca atcaaaatat tgccgcctat
     4141 gccgcgctaa ctcaggcaga aaataccact aataatagcg actatctgta tacgatgggg
     4201 gttagcgcca gattttaacg taacagtcac aattgaaacc attaaataac aataattgtg
     4261 gcgatagtgg gtgctaactt accaaataat aaatttggtg aataattgtc gcgtcattca
     4321 ttcctgaact aaggcatttc attccgttct gatggcattt catgccgttt ttccccaggc
     4381 ataaagtgca cttcgttatg gttgtcggca gagatttttc ctttttatta ctgcaggaat
     4441 actgccatga caccttcaaa ttatcagcgt acccgctggc tgacactcat cggtactatc
     4501 attacccagt ttgcgctggg gtcggtttat acctggagcc tgtttaatgg cgcgctttcc
     4561 gccaagctgg atgcgccggt aagccaggtc gctttctctt tcggcttgtt aagtctgggg
     4621 ctggcaattt cgtcttctgt tgcgggcaaa ttacaggaac gtttttgcgt taaacgcgtc
     4681 accatggctt ccggcatttt gctgggatta ggcttcttcc tgaccgcgca ttccaacaac
     4741 ctgatgatgc tgtggttaag cgccggtgtg ctggtcggtc tggcggatgg cgcgggttac
     4801 ctgctgacgc tctctaactg cgtgaagtgg ttcccggagc gtaaaggtct gatctccgcg
     4861 ttcgctatcg gttcttatgg tctgggtagc ctgggtttca aatttatcga cacgcagctg
     4921 ctcgaaacgg tgggtctgga aaaaacattt gtgatttggg gagcgattgc gctggtgatg
     4981 attgttttcg gcgcaacgtt aatgaaagac gcaccaaaac aggaagtgaa aaccagcaat
     5041 ggtgtggtgg agaaagatta cacgctggca gagtcgatgc gtaaaccgca gtactggatg
     5101 ttagcggtaa tgttcctgac tgcctgcatg agcggcctgt acgtgattgg ggtagcgaaa
     5161 gatatcgccc aaagtctggc acaccttgat gtggtttccg cagccaatgc agtaacggtt
     5221 atttccatcg ctaacctttc aggtcgtctg gtgctgggca ttctgtctga caaaattgcc
     5281 cgtatccgtg ttatcaccat tggtcaggtg atttcgctgg tgggtatggc ggccctgctg
     5341 tttgcaccat taaatgcagt gacgttcttt gcagcgattg cctgcgtggc atttaacttt
     5401 ggcggcacta ttaccgtctt tccatcactg gtcagtgagt tcttcggcct caataacctg
     5461 gcgaaaaact acggtgtgat ttacctcggt ttcggtatcg gtagcatttt tggttcgatt
     5521 atcgcctcac tgtttggcgg tttctatgtg accttctacg tgattttcgc cctgctgatt
     5581 ctgtcattgg cgctttctac gacgattcgt cagccagagc agaaaatgtt gcgtgaggcg
     5641 catggctccc tttaatgctc agccgcgagc tactgggctt cagcttccgt gtgcatcaaa
     5701 cgcatcaacg aaatgtataa acaatgactc gtaaaatttg ggccagttaa ctggcccttt
     5761 ttactgcttt ttgtaacgct aaagtctctt ttcaaacttg catttttgta aatttgtgct
     5821 tcatgcacac tctttcccca cactttttcg ctttgctgtg gtctacttat tcgcgcgtgt
     5881 agattttact tatctgacta cctccgcact tttcccctgc cgggcctgaa aagccgttaa
     5941 gcagggtgtt atcacctgtt tgtccagggt ttgtttgcat gagatacatc aaatcgatta
     6001 cacagcagaa gctgagcttt ttgcttgcaa tctatattgg cctttttatg aatggcgcgg
     6061 ttttttatcg ccgcttcggc agctatgcgc acgattttac cgtctggaaa ggcgtttctg
     6121 ctgttgttga actggccgcc accgtactgg tgaccttctt tttactacgt cttctatcgc
     6181 tgtttggccg ccgcagctgg cgtattctgg cgtcgctggt ggtgctcttt tccgcaggtg
     6241 ccagctatta catgaccttc cttaatgtgg tcattggtta tggcatcatc gcttccgtca
     6301 tgaccaccga tatcgacctg tcaaaagaag ttgttggtct gaactttatt ctctggttaa
     6361 tcgccgttag tgcattgcct cttatcctta tctggaataa ccgctgtcgc tacaccttgc
     6421 tccgacaact gcgaacccca gggcagcgta ttcgcagcct ggcggtcgtc gtactggcgg
     6481 gtattatggt ttgggcaccg attcgtttgc tggatatcca gcagaagaaa gtggagaggg
     6541 cgaccggcgt tgatttgccg agttatggcg gtgtcgtagc gaactcttat ctgccatcaa
     6601 actggctttc tgcgttgggg ctgtatgcct gggcgcgggt cgatgaatct tccgataata
     6661 attcattgct taatccggcg aagaagttta cataccaggc tccacaaaac gttgatgaga
     6721 cttatgtcgt gtttatcatc ggtgaaacca cgcgttggga ccatatgggt attttcggct
     6781 atgagcgtaa taccacgccg aaactggccc aggagaaaaa tctggcggcg ttccgtggtt
     6841 actcctgtga taccgcaacc aaactctcac tgcgttgcat gtttgtacgt caggggggcg
     6901 cggaagataa tccgcagcgc acattaaaag aacagaacat tttcgcggtt ctgaagcagt
     6961 taggattcag ttctgacctc tacgctatgc agagcgaaat gtggttctac agcaacacga
     7021 tggcggacaa cattgcttat cgtgagcaga ttggtgcgga gccacgtaat cgtggcaagc
     7081 cggtagatga tatgttgctg gtagacgaaa tgcagcaatc gctagggcgc aacccggatg
     7141 gtaagcatct gatcattctg cataccaaag gttcgcattt taactacacc cagcgttatc
     7201 cgcgtagctt cgcgcagtgg aagccggaat gtattggtgt tgatagcggc tgtaccaaag
     7261 cgcagatgat caactcctat gacaactcgg tgacctatgt ggatcacttt atctccagcg
     7321 tgattgatca ggttcgcgat aagaaagcga ttgtgttcta cgcagctgac cacggtgagt
     7381 caattaatga acgcgagcac ctgcacggca cgccgcgtga actggcaccg ccggagcagt
     7441 tccgcgtacc gatgatggtc tggatgtcag ataaatatct ggaaaatccg gccaatgcgc
     7501 aggcgtttgc gcagctgaaa aaagaagccg acatgaaagt gccacgccgt cacgtagagc
     7561 tgtacgacac catcatgggt tgtcttggct atacttcacc ggatggtgga attaacgaaa
     7621 acaacaactg gtgtcacatc ccgcagacaa aagaggcagc ggctaactaa gaggcctgct
     7681 gactttctcg ccgatcaaaa ggcattttgc tattaaggga ttgacgaggg cgtatctgcg
     7741 cagtaagatg cgccccgcat tcggtgattg gcgcagcttg gtagcgcact tcgttcggga
     7801 cgaaggggtc ggaggttcga atcctctatc accgaccaaa ttcgaaaagc ctgctcagcg
     7861 agcaggcttt tttgcatttg aagcccatga ggatgagaac ctccgggggc ggggaggttc
     7921 gactcgagcg aagcgagaga acgttgcgcc agcaacggcc cgcagggcga gccacgaagt
     7981 ggcgagtaat cctctatcac cgaccaaatt cgaaaagcct gctcaacgag caggcttttt
     8041 tgcatttgta gctcatgagg atgagaacct ccgggggcag ggaggttcga ctcgacgtag
     8101 gcctgataag acgcgccagc gtcgcatcag gcaacggcac cgcatttatt gtcggatgcg
     8161 gcgcgaccgc cttattcgac caacacacca cacattttcc tgccaacttt gcgccccata
     8221 tctcactatc cattaacgga tttgtgacag aaaccattgt ctttttttat atttgcttaa
     8281 atcttttttt gcgttactta tggctggcgc tggcattttc cgctgtgcgt tttagcgttc
     8341 atggtattaa tcgctcagat atccaccttt tcctcagatt tttttcatta aatgcataaa
     8401 acttaatcac caaatgttgc taaatattaa gctgattgtt ctggttatgt tggagtagca
     8461 caaatttctc tgctgcaaat agctgtttga agttttttta atctttgttt gtggattctc
     8521 accgttggtg taaatcccgg ttggctgtat tgacgttttc acattctgtt gacagattgt
     8581 aggtcacgag gggcatttta tggaggatcc gcactgttac actgatgtta attagtacgg
     8641 catccccacc tcataacgtt gacccgaccg ggcaaaaaac aaaaaaggtc aggcagcgac
     8701 aacccactgc aaagggttaa aacaacaaac atcacaattg gagcagaata atgcgtattt
     8761 ccttgaaaaa gtcagggatg ctgaagcttg gtctcagcct ggtggctatg accgtcgcag
     8821 caagtgttca ggctaaaact ctggtttatt gctcagaagg atctccggaa gggtttaacc
     8881 cgcagctgtt tacctccggc accacctatg acgcctcttc cgtaccgctt tataaccgcc
     8941 tggttgaatt taaaatcggc accactgaag tgatcccggg cctcgctgaa aagtgggaag
     9001 tcagcgaaga cggtaaaacc tataccttcc atctgcgtaa aggtgtgaag tggcacgaca
     9061 ataaagaatt caaaccgacg cgtgaactga acgccgatga cgtggtgttc tcgttcgacc
     9121 gtcagaaaaa cgcgcaaaac ccgtaccata aagtctctgg cggcagctac gaatacttcg
     9181 aaggcatggg cttgccggag ctgatcagtg aagtgaaaaa ggtggacgac aacacagttc
     9241 agttcgtact gactcgcccg gaagcgccgt tcctcgctga tctggcaatg gacttcgcct
     9301 ctattctgtc aaaagaatat gctgatgcga tgatgaaagc cggtacaccg gaaaaactgg
     9361 acctcaaccc aatcggaacc ggtccgttcc agttacagca gtaccaaaaa gattcccgta
     9421 tccgctacaa agcgtttgat ggctactggg gcaccaaacc gcagatcgat acgctggttt
     9481 tctctattac ccctgatgct tccgtgcgtt acgcgaaatt gcagaagaat gaatgccagg
     9541 tgatgccgta cccgaacccg gcagacatcg ctcgcatgaa gcaggataaa tccatcaacc
     9601 tgatggaaat gccggggctg aacgtcggct acctctccta taacgtgcag aaaaaaccgc
     9661 tggatgacgt gaaagttcgc caggcactga cctacgcggt gaacaaagac gcgatcatca
     9721 aagcggttta tcagggcgcg ggtgtatcag cgaaaaacct gatcccgcca accatgtggg
     9781 gctataacga cgacgttcag gattacacct acgatcctga aaaagcgaaa gccttgctga
     9841 aagaagcggg tctggaaaaa ggtttctcca tcgacctgtg ggcgatgccg gtacaacgtc
     9901 cgtataaccc gaacgctcgc cgcatggcgg agatgattca ggcagactgg gcgaaagtgg
     9961 gcgtgcaggc caaaatcgtc acctacgaat ggggtgagta cctcaagcgt gcgaaagatg
    10021 gcgaacatca gacggtaatg atgggctgga ctggcgataa cggggacccg gataacttct
    10081 tcgccaccct gttcagctgc gccgcctctg aacaaggttc taactactca aaatggtgct
    10141 acaaaccgtt tgaagatctg attcaaccgg cgcgtgctac cgacgaccat aacaaacgcg
    10201 ttgaactgta caagcaagcg caggtggtga tgcacgatca gactccggca ctgatcatcg
    10261 ctcactccac cgtgtttgaa ccggtacgca aagaagtcaa aggctatgtg gttgatccat
    10321 taggcaaaca tcacttcgaa aacgtctcta tcgaataatt aaaagccata caagactgat
    10381 ggcaaaggca aaactgcctg atgtgctccg cttatcaggc ctacgaaaat tctgcaatgt
    10441 attgaatttg cacgattttg taggccggat aaggcgttaa cgccgcatcc ggcataaaca
    10501 aagcgcactt tgtcaacaat ctgtataccc ggtggcgatg tgcctctgcg tgcagcgcca
    10561 tccggcagca atacttcatt ccctgtccga taagggcggg aattgatttg tgagcaatac
    10621 agacacgcag ttccaggctg cgggtcacta tagagaatcc gggttatgtt gcagtttatt
    10681 ctccgacgtt tgggactcgt catccccacg tttatcggta ttacccttct cacatttgcc
    10741 tttgtccaca tgatcccggg cgatccggtg atgatcatgg caggcgaacg cgggatctcc
    10801 cctgagcgtc acgcgcagct gctggctgaa ctcggcttag ataaaccgat gtggcagcag
    10861 tatctccatt acatttgggg cgtaatgcac ggcgatctag gcatttcaat gaaaagccga
    10921 attccagtat gggaagagtt cgtgccgcgc tttcaggcca cgctggaact tggcgtctgc
    10981 gcgatgattt ttgctacggc agtcggtatt ccggttggtg tgctggctgc ggttaaacgc
    11041 ggttccattt tcgatcacac tgcagttggc ctggcgctga ccggctactc gatgccgatc
    11101 ttctggtggg gcatgatgct gatcatgctg gtttcggtgc actggaacct gacgcccgtc
    11161 tccggtcgcg tgagcgatat ggtgttcctc gatgactcca atccgttaac cggttttatg
    11221 ctgatcgaca ccgccatctg gggtgaagat ggcaacttta tcgacgccgt tgcccatatg
    11281 atcttaccgg ctattgtgct gggtactatc ccgctggcgg ttatcgttcg tatgacgcgc
    11341 tcttcgatgc tggaagtgct gggtgaagat tacatccgca ccgcgcgcgc caaagggttg
    11401 acccgcatgc gggtgattat cgtccatgcg ctgcgtaacg cgatgctgcc ggtagtgacc
    11461 gttatcggcc tgcaggtggg aacattgctg gcgggggcga ttctgaccga aaccatcttc
    11521 tcgtggcccg gtctgggacg ctggttgatt gacgcactgc aacgccgcga ctatccggta
    11581 gtgcagggcg gcgtattgct ggtggcgacg atgattatcc tcgtcaacct gctggtcgat
    11641 ctgctgtacg gcgtggtgaa cccgcgtatt cgtcataaga agtaagggga catcatgtca
    11701 caggttactg aaaataaagt gattagcgca ccggtgccga tgaccccgtt acaggagttc
    11761 tggcactatt ttaaacgcaa caaaggcgcg gttgtcgggc tggtttacat cgtcatcgtg
    11821 ctgtttatca cgatctttgc caactggatt gcgccttata acccggcgga tcagttccgc
    11881 gatgcgctgc tcgccccgcc agcctggcag gaaggcggca gcatggcgca cttgctgggc
    11941 accgatgacg taggccgtga tgtgctgtcg cggctgatgt acggcgcgcg cttgtcgctg
    12001 ctggttggct gtctggtggt ggtgttatcg ctgattatgg gcgttattct cggcctgatc
    12061 gccggttact tcggcggcct ggtcgataac atcatcatgc gcgtggtcga tatcatgctg
    12121 gcgctgccaa gtctgctgtt ggcactggtg ctggtggcga ttttcggccc gtcgattggt
    12181 aacgccgcgc tggcactgac cttcgttgcc ttgccgcact atgtgcgctt aacccgcgcc
    12241 gccgtgctgg tggaggtgaa tcgcgattac gttaccgcgt ctcgcgtggc aggtgccggg
    12301 gcgatgcgtc agatgtttat taacatcttc ccgaactgcc tggcgccgct gattgttcag
    12361 gcgtcgctcg gtttctctaa cgccattctc gatatggccg cccttggctt cctcggcatg
    12421 ggagcgcagc cgccaacacc agagtggggc accatgctct ccgacgtgtt gcagttcgcg
    12481 caaagcgcct ggtgggtcgt gaccttcccg ggtctggcga tcctgctgac ggtgctggca
    12541 tttaacctga tgggtgacgg tctgcgtgac gcgctcgatc ccaaactgaa gcagtaagag
    12601 gttcgagatg gcgttattaa atgtagataa attatcggtg catttcggcg acgaaagcgc
    12661 accgttccgc gccgtagacc gcatcagcta cagcgtaaaa cagggcgaag tggtcgggat
    12721 tgtgggtgag tctggctccg gtaagtcggt cagttcactg gcgattatgg ggctgattga
    12781 ttatccgggc cgcgtaatgg cggaaaagct ggtgtttaac ggtcaggatt tgcagcgtat
    12841 atcagaaaaa gagcgccgca acctggtggg tgccgaagtg gcgatgatct tccaggaccc
    12901 gatgaccagc cttaacccgt gctacaccgt gggtttccag atcatggaag cgattaaggt
    12961 gcatcagggc ggtaataaga gcactcgccg tcagcgggcg attgacctgc tgaatcaggt
    13021 cggtattccc gatccggcat cgcgtctgga tgtttacccg catcagcttt ccggcggcat
    13081 gagccagcgc gtgatgatcg ccatggcgat tgcctgtcgg ccaaaactgc tgattgccga
    13141 tgagccgacc actgcgctgg acgtgaccat tcaggcgcaa atcatcgaac tgttgctgga
    13201 gctacagcag aaagagaaca tggcgctggt gttaattacc catgatctgg cgctggtggc
    13261 ggaagctgcg cataaaatca ttgtgatgta tgcaggccag gtggtggaaa cgggcgatgc
    13321 gcacgccatc ttccatgcgc cgcgtcaccc gtatactcag gcattgctgc gtgcgctgcc
    13381 agaatttgct caggacaaag aacgtctggc gtcgttgcca ggtgtcgttc ccggcaagta
    13441 cgaccgcccg aacggctgcc tgcttaaccc gcgctgcccc tatgccactg acagatgtcg
    13501 cgctgaagaa ccggcgctga atatgctcgc tgacgggcgc cagtccaaat gccattaccc
    13561 acttgatgat gccgggaggc cgacactatg agtacgcaag aggccaccct gcaacaaccg
    13621 ctgttgcagg ctatcgacct gaaaaaacat tatccggtga agaaaggcat gttcgcgccg
    13681 gaacgtctgg ttaaagcgct ggatggcgtt tcgtttaacc ttgaacgtgg caaaacgctg
    13741 gcagtagtgg gcgaatctgg ctgcggtaaa tcgaccctcg gtcggttgct gacgatgatt
    13801 gaaacgccca ccggtggcga gctgtattac caggggcagg atctgcttaa gcacgatccg
    13861 caggcgcaga agctgcgtcg gcagaaaatc cagatcgtct tccagaaccc ttacggttcg
    13921 ctgaatccgc gtaaaaaagt cgggcaaatt cttgaagagc cgctgctgat caacaccagc
    13981 ttaagcaaag aacagcgtcg ggaaaaagcc ctgtcgatga tggcgaaagt cggcctgaaa
    14041 accgagcact atgaccgcta tccgcatatg ttctccggcg gtcagcgtca gcgtatcgcc
    14101 atcgcccgtg gtctgatgct cgacccggat gtggtgattg ccgatgaacc ggtttccgcg
    14161 ctggatgttt cagtgcgcgc gcaggtgctg aatctgatga tggatttgca gcaggagttg
    14221 gggctgtctt atgtctttat ctcccacgac ctgtcggtgg tggagcacat tgctgatgaa
    14281 gtgatggtga tgtacctggg ccgctgcgtg gagaagggaa cgaaagacca aatcttcaat
    14341 aacccgcgcc atccgtacac tcaggcgcta ctttccgcga cgccgcgcct gaacccggac
    14401 gatcgccgcg agcgcatcaa gctcagcggt gaactaccaa gcccactgaa tccaccgccg
    14461 ggttgcgcct tcaacgcccg ctgtcgtcgg cgcttcggcc cctgcaccca gttgcagccg
    14521 cagctaaaag actacggcgg tcaactggta gcttgttttg ctgttgatca ggatgaaaat
    14581 ccgcagcgtt aactcacaaa accggggcca tgcgctccgg tttactcaat cagcttcaac
    14641 agtggcgaca agcatagcaa aatgccgtac agcagaatca gccaggcttt aaatccgcga
    14701 agtttttcca gttgtgcgac tttatagatc aggaaaaacg gaatgaggca cgacacaata
    14761 ccatataacg ggcttcccaa ctgaaagaac accagcaccg atacacgaaa cgaaacccaa
    14821 atcgtcaacg taatgacgat aaaagcgcag atcgccagag tcagcacgcg tgagttaatt
    14881 ttcttggtat caataattcg gcttaacaga ttgagaataa tgcctttaat ggcctcgtgg
    14941 aaaccgagat aaatgccaaa gaatgcggtc agtacggcaa agatattaag caccgtagag
    15001 gtgatatgaa tgatatgccc agggatcacc tgcgcggcca gcgccagtgc tgagatattt
    15061 tgttcaaagg cagaaacggc ttcttcgtgg ctaattgaga aggtaaacga aaaggcaaaa
    15121 aacaggatca ccgcgatgag cgtgatataa ctaattcggt gggtacgcag cgcgagccgg
    15181 gttgccagta ctttatccgc ttcccgttta cgataggcaa tattcattgg gttaagtacc
    15241 tgaataaata ctgcagaaaa gaagcaaaat ggaatggtaa gcaagacatc gcggaaaaag
    15301 acggaggctt gcgggaaggc ggttatattg gcgaaattcc agtgcgggat catcgcaaaa
    15361 ccgaacacga caataatccc tactttgacc accaccattg gcccggaaat cttaaacagt
    15421 aatcgttcac caccagacgc aatcgttacc agtacggcga aaatagcgac tttataaagt
    15481 agagattgtg aaagatcggc atcggttaaa ccgaaggttt tcaggtacga ggcgctgtcg
    15541 aaaaccacgg agagagagta gataaaaatc ccgtggataa tcatcaaaaa gtagataacc
    15601 ccgaggaaaa ttccccagtt cttccccagg taatgactga taatatcggt gtagtcatta
    15661 caggaatcgc tttcagaaag ggtttttaaa taaatgtcct gcactaccca ggtggcagga
    15721 taagcaatga tcgctgcggt aataaatacc caaattccct tcaagccaat ttgtactggc
    15781 atcagcacgg ttccggcacc aatcgccatg cctatgcata ataaaaccca gccgaagtcg
    15841 tagcgtgtaa acggcaattt ctgattgtgt ttcgatagtg tgttgtgctg cataaagtcc
    15901 tgtccagaaa gaagtcggcc tcgcaaaaat gaataaaccc agtttctctt gcgcaacagg
    15961 tggagaagtc gaatacgcag tatgtaaaaa atgaggcaac agattcactg gggaggtcgc
    16021 gaggaggcgc aattctacaa aattgcgcat ttcactacca gtctggagtc agcccggata
    16081 tgaactgcaa ctattgcaaa taaagaaatt aagctggaag gcatcgcgca atgaggaaga
    16141 ttgccgggtg ctgacagtaa aaagcaatcg taaaatcagc ggcaagccga aagggttccg
    16201 gtgaggcgca atgttgcggg ggctttatcc ctggtggcat tggttgctgg agagagaaaa
    16261 cccccgcacg ttgcaggtat gtacctgaca acataacggg ggctaatctt gaacctagac
    16321 cactcaagaa tagccgcgaa acgttgtcat tacaacacag gcggctatat gacgctcgca
    16381 gagctgggca tggccttctg gcatgattta gcggctccgg tcattgctgg cattcttgcc
    16441 agtatgatcg tgaactggct gaacaagcgg aagtagcgtg tcatgtgggc gtcaggctgc
    16501 cgtaatggca atttgcgccc ggaccaggcc gcaggagtga aactctgcgg cctttttcgt
    16561 aagaaaaatg caacggttgc aagcggataa tattctgcgg aatgcgcagc acagagtgaa
    16621 gatgagacgg attccggaac agaaaaacag tcgtagaatc agcgtcaagc cggaaggatt
    16681 ccggtgaggc gcaatgttgc gggggcttaa tccctggtgg cattggttgc tggagagaga
    16741 aa
//
LOCUS       DADMKF010000083        16696 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.084, whole genome shotgun sequence.
ACCESSION   DADMKF010000083 DADMKF010000000
VERSION     DADMKF010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16696
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.084"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            96..1235
                     /gene="arnB"
                     /locus_tag="JNC24_002512"
     CDS             96..1235
                     /gene="arnB"
                     /locus_tag="JNC24_002512"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HAY9159234.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLAKLEHLNTHRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            1239..2207
                     /gene="arnC"
                     /locus_tag="JNC24_002513"
     CDS             1239..2207
                     /gene="arnC"
                     /locus_tag="JNC24_002513"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HAY9159235.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            2207..4189
                     /gene="arnA"
                     /locus_tag="JNC24_002514"
     CDS             2207..4189
                     /gene="arnA"
                     /locus_tag="JNC24_002514"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HAY9159236.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPAEKAFYGS
                     VARLAAERGIPVYAPDNVNHPLWVERIAQLSPEVIFSFYYRHLICDEILQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRVAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPAIKHGNILEIAQRENEATCFGHRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISVAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQMLGLVQGSRLNSQPACTARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSSYYGKGYQDVEHRKPSIR
                     NARRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            4186..5076
                     /locus_tag="JNC24_002515"
     CDS             4186..5076
                     /locus_tag="JNC24_002515"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HAY9159237.1"
                     /translation="MTKAGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            5076..6728
                     /gene="arnT"
                     /locus_tag="JNC24_002516"
     CDS             5076..6728
                     /gene="arnT"
                     /locus_tag="JNC24_002516"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HAY9159238.1"
                     /translation="MKSVRYLIGLFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFASLAMLMAHYALLA
                     AKNNPLALRINGWINIAFGFTGIIATFVVSPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIDRQERLVLIQYRPK"
     gene            6725..7060
                     /gene="arnE"
                     /locus_tag="JNC24_002517"
     CDS             6725..7060
                     /gene="arnE"
                     /locus_tag="JNC24_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HAY9159239.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            7060..7446
                     /gene="arnF"
                     /locus_tag="JNC24_002518"
     CDS             7060..7446
                     /gene="arnF"
                     /locus_tag="JNC24_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HAY9159240.1"
                     /translation="MGLMWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(7440..7706)
                     /gene="pmrD"
                     /locus_tag="JNC24_002519"
     CDS             complement(7440..7706)
                     /gene="pmrD"
                     /locus_tag="JNC24_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HAY9159241.1"
                     /translation="MEWLVKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKVGD
                     LLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCKVAGKTQ"
     gene            complement(7750..9171)
                     /gene="menE"
                     /locus_tag="JNC24_002520"
     CDS             complement(7750..9171)
                     /gene="menE"
                     /locus_tag="JNC24_002520"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000577609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HAY9159242.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELATGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQRVEGAHAATWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAQGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGSPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVPVADKEFGH
                     RPVAVVEYDQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     DYEIISFTSQFCHSIAVDNRFIC"
     gene            complement(9168..10130)
                     /gene="menC"
                     /locus_tag="JNC24_002521"
     CDS             complement(9168..10130)
                     /gene="menC"
                     /locus_tag="JNC24_002521"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HAY9159243.1"
                     /translation="MRSAQVYRWQIPMDAGMVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQETWEEAQIVLLAWVNNWLAGDGELPQMPSVAFGVSCALAELADTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSPLPLVDADVLEQLL"
     gene            complement(10130..10987)
                     /gene="menB"
                     /locus_tag="JNC24_002522"
     CDS             complement(10130..10987)
                     /gene="menB"
                     /locus_tag="JNC24_002522"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HAY9159244.1"
                     /translation="MIYPDEAMLYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNVGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(11002..11760)
                     /gene="menH"
                     /locus_tag="JNC24_002523"
     CDS             complement(11002..11760)
                     /gene="menH"
                     /locus_tag="JNC24_002523"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HAY9159245.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VYLPGHGGSAAISVDGFDDVTDLLRKTLVSYNILNFWLVGYSLGGRVAMMAACQGMPG
                     LCGVVVEGGHPGLQNAEQRAERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(11757..13427)
                     /gene="menD"
                     /locus_tag="JNC24_002524"
     CDS             complement(11757..13427)
                     /gene="menD"
                     /locus_tag="JNC24_002524"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HAY9159246.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     SGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALVLLRQVSAPLILIVVNNNGGQIFSLLPTPKSERERFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETALADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(13516..14811)
                     /gene="menF"
                     /locus_tag="JNC24_002525"
     CDS             complement(13516..14811)
                     /gene="menF"
                     /locus_tag="JNC24_002525"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HAY9159247.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIKAREFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADNVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(14890..15195)
                     /gene="elaB"
                     /locus_tag="JNC24_002526"
     CDS             complement(14890..15195)
                     /gene="elaB"
                     /locus_tag="JNC24_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HAY9159248.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKARAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(15250..15711)
                     /locus_tag="JNC24_002527"
                     /pseudo
     CDS             complement(15250..15711)
                     /locus_tag="JNC24_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311182.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            15776..16693
                     /gene="rbn"
                     /locus_tag="JNC24_002528"
     CDS             15776..16693
                     /gene="rbn"
                     /locus_tag="JNC24_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HAY9159249.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPMEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
BASE COUNT         3855 a         4549 c         4285 g         4007 t
ORIGIN      
        1 ctatccttaa aatttcgctc caaatggcaa aatatacaca acactcttta tagcaaatat
       61 aagtggacag gtattcaatg gcggaaggaa aagcaatgtc agaatttttg cctttttcgc
      121 gaccagcaat gggcgtggag gaactcgctg cagttaaaga ggttctcgaa tccggttgga
      181 tcacaaccgg tccgaagaat caggcgcttg agcaagcctt ttgccagttg acgggaaatc
      241 agcatgccat cgcggtcagt tcagccaccg ccggaatgca tatcacgcta atggcgttgg
      301 aaattggcaa gggcgatgaa gtgattacgc cttccctgac ctgggtttca accctcaata
      361 tgatttcctt gttgggtgca acgccggtaa tggtggatgt cgaccgcgat acgctgatgg
      421 tcacgcctga agcgatcgag tcagccatta cgccacgcac taaagccatc attccggtgc
      481 attatgccgg tgcgccagca gatattgacg ccattcgcgc cattggcgaa cgttacggca
      541 tcgcagttat cgaagatgct gcccatgccg tcggtacgta ttacaaaggg cgacatattg
      601 gcgcaaaagg taccgctatt ttttcatttc atgccattaa aaatattacc tgtgcggaag
      661 gtggcctgat tgtaactgat aatgaaaacc ttgcccgcca gctacggatg ctgaaatttc
      721 acggtctggg tgtcgatgcc tatgacagac aaacctgggg ccgtgcaccg caggctgaag
      781 tcttaacacc gggctataag tacaatctga ccgatattaa cgccgcgatt gccctgacac
      841 agttagcaaa attagagcac ctcaacaccc atcggcgcga aattgcccag caatatcagc
      901 aagcactggc agctctcccc tttcagccat taagccttcc cgcctggccg cacgttcacg
      961 cctggcatct gtttattatt cgtgtcgatg aacaacgttg tggtatcagt cgcgatgcgt
     1021 tgatggaagc gttaaaagaa agaggcattg gtaccgggtt acatttccgc gccgctcaca
     1081 cacaaaaata ttatcgcgag cgttttccca cgctgtcgtt accgaatacc gaatggaata
     1141 gcgaacgcat ctgttctttg ccgctgttcc cggatatgac taccgccgat gccgaccgtg
     1201 tcatcacagc ccttcagcaa ctcgcaggac aataagccat gtttgaaatc caccctgtta
     1261 agaaagtctc ggtggttatt cccgtttata acgagcagga aagcttaccg gaattaatca
     1321 ggcgcaccac cacagcctgt gaatcgttgg ggaaagagta tgagatcctg ctgattgatg
     1381 acggcagtag cgataattcc gcgcatatgc tggtcgaagc ctcacaagcg gagaacagcc
     1441 atattgtgtc tattttgctt aaccgcaatt acgggcaaca ttcagcgatt atggcgggat
     1501 tcagtcacgt tactggcgac ttaattatta cccttgatgc cgatctccag aatccgccag
     1561 aagaaatccc ccgcctggtg gcaaaagccg atgaaggtta cgacgtggta gggactgtac
     1621 gccagaaccg ccaggacagc tggtttcgta aaacagcttc gaagatgatt aacaggctta
     1681 ttcagcgcac caccggcaaa gcgatgggtg actacggttg tatgctgcgc gcctatcgcc
     1741 gtcatattgt cgatgcgatg ttgcactgcc atgaacgtag cacctttatc ccgattctgg
     1801 cgaatatctt cgcccgccgt gccattgaaa ttccagtaca tcatgccgag cgtgagtttg
     1861 gtgaatccaa atacagtttt atgcgcctga ttaatttgat gtacgacctg gtgacctgcc
     1921 ttaccacaac gccgctacgt atgctgagtc tgctcggcag cattattgcg attggaggtt
     1981 ttagcattgc ggtgctgctg gtgattttac gcctgacctt cggaccacaa tgggcggcag
     2041 aaggcgtctt tatgctattt gccgtgctgt ttacttttat tggcgctcag tttatcggca
     2101 tgggattgct cggtgaatat atcggcagga tctacaccga tgtccgcgcc cgcccccgct
     2161 attttgttca gcaagttatc cgtccatcca gcaaggaaaa tgaataatga aaaccgtcgt
     2221 ttttgcctac cacgatatgg gatgcctcgg tattgaagct ctgctggctg ccggttacga
     2281 aattagcgcc atttttaccc ataccgataa tcccgctgaa aaagcctttt atggttcggt
     2341 ggctcgtctg gcggcggaaa gaggcattcc agtttatgcg ccggataacg ttaatcatcc
     2401 gctgtgggtg gaacgcattg cccaactctc accagaggtg attttctctt tttattatcg
     2461 ccatcttatt tgcgacgaga ttttgcagct cgctcccgca ggtgcattta atctgcacgg
     2521 ttcgctgtta ccaaaatatc gtggtcgcgc gccgctgaac tgggtgctgg tgaacggtga
     2581 aacggaaacg ggcgttacat tgcaccgaat ggtaaaacgt gccgatgctg gggccattgt
     2641 ggcgcaactg cgcgttgcca ttgcgccaga cgatatcgct attacgctgc atcataaatt
     2701 gtgccatgcc gcgcgccagc tactggaaca gacattaccc gccattaaac acggtaatat
     2761 tctggaaatt gcccagcgcg aaaacgaagc cacctgtttt ggtcacagaa cgccagatga
     2821 cagtttcctt gaatggcata aaccggcatc cgtactgcac aacatggtac gtgccgttgc
     2881 cgatccgtgg ccgggtgcct tcagctatgt tggcaatcag aaattcaccg tctggtcgtc
     2941 gcgtgttcat cctcatgcca gcaaagcaca gccggggagc gtgatttctg ttgcgccact
     3001 gctgattgcc tgtggcgatg gcgcgctgga aatcgtcacc ggacaggcgg gcgacggcat
     3061 tactatgcag ggctcgcaat tagcgcagat gctgggcctg gtgcaaggtt cacgcttgaa
     3121 tagccagcct gcctgcaccg cccgacgccg tacccgggta ctcatcctcg gggtgaatgg
     3181 ctttattggc aaccatctga cagaacgcct gctgcgcgaa gatcattatg aagtttacgg
     3241 tctggatatt ggcagcgatg cgataagccg ttttctgaat catccgcatt ttcactttgt
     3301 cgaaggcgat atcagtattc attccgaatg gattgagtat cacgtcaaaa aatgtgatgt
     3361 cgtcttgccg ctggtggcga tagccacgcc gattgaatat acccgcaacc cgctgcgcgt
     3421 atttgaactc gattttgaag agaatctgcg cattatccgc tactgcgtta agtaccgtaa
     3481 gcgaatcatc ttcccgtcaa cttcagaagt ttatgggatg tgtagcgata aatacttcga
     3541 tgaggaccat tctaatttaa tcgtcggccc ggtgaataaa ccacgctgga tttattcggt
     3601 gtcaaaacaa ttacttgatc gggtgatctg ggcctatggc gaaaaagagg gtttacagtt
     3661 caccctcttc cgcccgttta actggatggg accacgactg gataacctta atgcagcgcg
     3721 aattggcagc tcccgcgcta ttacgcaact cattctcaat ctggtagaag gttcaccaat
     3781 taagctaatt gatggcggaa aacaaaaacg ctgctttact gatatccggg acggtatcga
     3841 ggcgttgtac cgcattatcg aaaatgcggg aaatcgctgc gacggcgaaa ttatcaacat
     3901 tggcaatcct gagaacgaag cgagcattga ggaactgggc gagatgctgc tggcgagctt
     3961 cgaaaaacat ccgctgcgcc atcacttccc accgtttgcg ggctttcgtg ttgtcgaaag
     4021 tagcagctac tacggcaaag gatatcagga cgtagagcat cgtaaaccga gcatccgcaa
     4081 tgcccgccgc tgcctggact gggaaccgaa aattgatatg caggaaacca tcgacgaaac
     4141 gctggatttc ttcctgcgca ccgttgatct tacggataaa ccatcatgac caaagccggc
     4201 ttacgcattg atgtcgatac ctttcgtggc acccgtgaag gcgtgccacg tctgctggaa
     4261 atcttgagta agcataatat tcaggccagc atttttttca gcgtcggccc ggacaatatg
     4321 ggccgccatc tctggcgact ggtgaagcca cagtttttgt ggaagatgct gcgctcaaac
     4381 gcggcatcgc tttatggctg ggatatttta ctggcaggta cggcctggcc aggtaaagag
     4441 attggtcatg ccaatgccga tatcattcgt gaagcggcta aacatcacga agtcggcctg
     4501 cacgcctggg atcaccatgc ctggcaagcc cgtagcggta actgggatcg gcaaacaatg
     4561 atcgacgata ttgcacgcgg tcttcgcact ctggaagaga ttatcggtca accggtaacc
     4621 tgttctgccg ctgcgggctg gcgtgccgac cagaaggtga tcgaagcaaa agaagcgttc
     4681 catttgcgct acaacagcga ttgtcgtggg gccatgccgt tccgtccact gctcgaatca
     4741 ggaaaccctg gcactgcgca aattccggtg accttaccca cctgggatga agtgattggt
     4801 cgggatgtga aagcagaaga ttttaacggt tggttactca accgcatcct gcgagataaa
     4861 ggcacgccgg tttataccat tcatgcagaa gttgaaggct gcgcttatca gcataatttt
     4921 gtggatctcc tcaaacgcgc agctcaggaa ggcgtgacat tttgcccttt aagcgaactg
     4981 ttatcagaga cgttgccgct cggacaagtt gttcgcggaa atattgccgg acgtgaaggc
     5041 tggctgggtt gccaacaaat tgcgggtagt cgctgatgaa atcggtacgt taccttatcg
     5101 gcctcttcgc gtttattgcc tgctattacc tgttaccgat cagcacgcgt ctgctctggc
     5161 aacccgatga aacgcgttat gcggaaatca gtcgagaaat gctggcatcc ggcgactgga
     5221 ttgtgcccca tctgttaggg ctacgttatt tcgaaaaacc cattgccgga tactggatta
     5281 acagcattgg gcaatggcta tttggcgcga ataactttgg tgtgcgggca ggcgttatct
     5341 ttgcgaccct gttaactgcc gcgctggtga cctggtttac tctgcgctta tggcgcgata
     5401 aacgtctggc tctactcgcc acagtaattt atctctcatt gtttattgtc tatgccatcg
     5461 gcacttatgc cgtgctcgat ccgtttattg cattctggct ggtggcggga atgtgcagct
     5521 tctggctggc aatgcaggca cagacgtgga aaggcaaaag cgcaggattt ttactgctgg
     5581 gaatcacctg cggcatgggg gtgatgacca aaggttttct cgcccttgcc gtgccggtat
     5641 taagcgtgct gccatgggta gcgacgcaaa aacgctggaa agatctcttt atttacggct
     5701 ggctggcggt tatcagttgc gtactgacgg ttctcccctg gggactggcg atagcgcagc
     5761 gggagcctga cttctggcac tattttttct gggttgagca tattcaacgc tttgcactgg
     5821 atgatgccca acatagagct ccgttctggt actacgtgcc ggtcatcatt gccggtagcc
     5881 tgccgtggct gggattactc cccggtgcac tgtacacagg ctggaaaaac cgcaagcatt
     5941 ccgcaaccgt ctatttgttg agctggacga taatgccgct gctgtttttc tccgtcgcta
     6001 aaggtaaatt gcccacctat attctttcct gctttgcatc tctggcaatg ctgatggcgc
     6061 attacgcttt gctggcagca aaaaataatc ctctggcgct gcggattaat ggctggatta
     6121 acatcgcttt tggcttcact ggcattattg ccacatttgt ggtctccccg tggggaccaa
     6181 tgaacacgcc ggtgtggcaa accttcgaga gctataaagt cttttgtgcc tggtcgattt
     6241 tttcgctatg ggcatttttc ggctggtaca ccttaacaaa cgtcgaaaag acctggtctt
     6301 ttgccgcgct ttgcccgctg gggctggcgt tgctggtagg attttcaatt cctgacagag
     6361 tcatggaagg aaaacatccg caattttttg tcgagatgac acaagaatca cttcagccaa
     6421 gccgctatat tcttactgat agcgtcggtg ttgccgcagg tctggcatgg agcctgcaac
     6481 gcgatgacat catcatgtat cgccagacag gtgagttgaa atacggcctt aattatccgg
     6541 atgcgaaagg gagatttgtc agcggtgatg agttcgcaaa ctggcttaat caacatcgtc
     6601 aggaggggat tattacactc gtgctttcgg ttgaccgcga tgaagatatc aacagtctcg
     6661 ccattccgcc cgcagatgcc atcgatcgtc aggagcgtct ggtgctgatt cagtatcgtc
     6721 ccaaatgatc tggctaacat tagtctttgc cagcttgctt agcgttgccg ggcagttgtg
     6781 tcagaaacag gcaacctgct ttgtggcgat aaacaaacgg cgcaaacata tcgtgctgtg
     6841 gctgggactg gcgctggctt gtcttggtct tgccatggtg ctctggctgc tggtcttgca
     6901 gaacgtaccg gtaggcattg cctacccgat gttaagtctg aattttgtct gggtgacgct
     6961 ggctgcagta aaactgtggc acgaaccggt atcgccgcgt cactggtgtg gggtggcgtt
     7021 cattattggc ggcattgtga tcctcgggag tacggtgtaa tgggcctgat gtggggatta
     7081 ttcagcgtca ttattgcctc agttgcgcaa ttaagcctgg gttttgcggc gagtcatctg
     7141 ccgccgatga cgcacttatg ggattttatc gcggctctgt tagcttttgg cctggatgcc
     7201 cggatactgt tactcgggct gctgggttat ctgctttccg ttttttgttg gtataaaacg
     7261 ctacataaac ttgcgctaag caaagcctat gccttgctca gtatgagtta tgtgctggta
     7321 tggattgctt caatggtact tcccggctgg gaagggactt tttcgctgaa agcactactg
     7381 ggagtagcct gtattatgag cgggttgatg ctgattttcc tgcccacgac aaaacaacgt
     7441 tactgagttt tccctgccac tttacactgc cgttcccact catcggggct ataactgctt
     7501 gccgagagga ctttcaccgt atgcagcttt tcacgattaa tacaataaag cgcattctgc
     7561 aaaggcgaga gtaaatcccc cactttcacg gcgaagtcac tcttcacttc ggcgatcatt
     7621 tttatcgcgc cgccagcatc gcagagcata agtacatgtc tgttatcttg tttattgcag
     7681 cacgattttt tgaccagcca ttccattgca ttatcctgtt tgctaagagt tttcacatca
     7741 atttacagat taacagataa acctattatc aacggcaatg gaatggcaaa attgcgaagt
     7801 gaaactaatg atttcgtaat cctggcgttg cacccactct tttaacgcct gacgtgaaat
     7861 tttaataccg ccgtttttca gctccggcgg taaagttagc cagcgcaccg gttgttgaaa
     7921 acgagccagc ttatctttca cccattcgcc aagatcaacg gtttgctggt catactccac
     7981 caccgctacc ggtcgatgac caaactcctt gtcggcaacg gggacgataa acacctgcaa
     8041 aaccgcagga tgtgcagcaa ttacgcgctc gacttcttcc ggctgaatac cctctccgcc
     8101 actgaagaac agattgtcta aacgtccgac aatggtcagc ttgccattat gcatctcacc
     8161 gcgatcgcgc gtagcgtacc agccttcgtc attaaccagt gaaaccagtt gcccgttacg
     8221 ccagtaacct tctgccatac tggcagcccg cagccacact tcattattaa cgattttcac
     8281 ttcccgaccc ggcagcggcg aaccaacgtc tgccaggccg tcggcttctt tcgcacacac
     8341 cgtggaggca aactcggtca gaccatagcc gcaaaagcaa cgaatcccct gctcgcgcgc
     8401 ctgttccgtc aactcgaccg ggatagccgc gccgccaagt aacaccgctt tcagggaaac
     8461 ggaactgcgg ttaaccagca aacgccagag ttgtgttggc accagtgaag cgtgagtaca
     8521 gcctgccagc atttgctcca atggctgttt atcacgcacc gtcatccgcg caccagcgta
     8581 taaccagcgc cacataattc cctgaccgga gacgtgaaac agcggtaaag agagcaacca
     8641 gtcatcgtga tcgccaaacg gaatcagcga taacacacct tgcgcactgg caagatgggc
     8701 ctgataagta tggacagcgg ctttcggcaa accggtagaa cccgaggtca acgtcattga
     8761 gcacagacgc gtcggctgcc acgtagcggc atgtgcgcct tcaacccgct gaatgtgcag
     8821 cgacgttaat gccggaaacg tgttttcccc atccggcacc agcgcaaatt gcagcgtcag
     8881 attgggcagc aattcttcaa gcaacggttg cggcagctga gggttcacgg gcaacacccg
     8941 cgccccgcat tgcagtaacg ccagccaggc gagcagcgtt tgcggcgtat tccacgcccg
     9001 caacatcacg ccgctgccct caaccacacc ctgcgccgca aagcccgtag ctaactcatc
     9061 gacgcgagca caaagctcgc gccagttgag ttgctcgtca ttaagacgta aggcgatggc
     9121 ttctccccgc acttgccgcc agtgacgcca cggccagtca gagaagatca taacaactgc
     9181 tccagtacat cagcgtccac taacggcagt gggctccccg gccagcgacg aacctgctgc
     9241 gcctgcatca gatccagcgt gtccagtccc ggaatggtgt ctggcgttaa ccaggcagca
     9301 atccgcgcca gttgcgttag acctaagctc gactcaatgg aagaactgat caccgccgtc
     9361 agccccagcg catgcgccgc ctgtacctgc tcgcgtactt tttccagact gcccgtgagc
     9421 gtgggtttga taactaccgc gcgcacgccc tcttcagcca caaaggcaaa atccggctcg
     9481 cgcaggcttt catcccaggc aatggcaatg ccggtttcac gggcaaacgc tcgcgaatca
     9541 tcgcgggttt tgcacggctc ttcgagaaac gcgatgcggt ggcgataatc cgggttaaca
     9601 tatttggcaa actgctgacc tttcagcggt gtccaggcgc gatttgcgtc aagacgcaaa
     9661 tgcagatccg gaattgcctc cagcaacaga ttaaccacca tgccgtcgcg cacggcttcg
     9721 tacaatccca ctttgacctt cgccactttc tcgcctggca tatcagcaag tttgaggatc
     9781 agatcgtcag gatcgccatt acacagtggt gccgcacggt aattggccgc ctgcgggagc
     9841 gtatctgcca gctccgccaa tgcacagctt acgccaaagg caacggaagg catctgcggt
     9901 agctcgccat cgcctgccag ccagttattt acccaagcaa gcagcacaat ttgcgcctct
     9961 tcccaggttt cctgactgaa gcccggcagt ggggagatct ccccccaccc ttcgcgctcg
    10021 ccttcacgca ggcaaacata cagcccgtcg cgggttttta accgcctgtc gcgcagaacc
    10081 atccccgcgt ccatggggat ctgccagcgg tatacctgcg cgctacgcat tacggattcc
    10141 gtttaaattt gctgaaatca ggctgacgtt tctggttgaa agcgttgcga ccttcctgac
    10201 cttcttccgt catgtagaac agcatggtgg cgttgcccgc cagctcctgc agacccgcct
    10261 gaccgtcaca gtcggcgttc agcgcagctt tcaggcagcg cagcgccatc gggctgtttt
    10321 gcagcatttc gcggcaccaa cggacggttt ctttttccag atccgccagc ggtaccacgg
    10381 tgttgacaag gcccatatcc agcgcctgtt ttgcatcgta ctgacggcac aggaaccaga
    10441 tttcacgcgc ttttttctgt ccgacgatgc gagccatgta ggaagcgccc cagccgccgt
    10501 cgaaggaacc gactttcggg ccagtctgac cgaagatggc attatctgcc gcgatagtca
    10561 ggtcgcacat catgtgcaga acgtgaccac cgccgatgga gtagccagcc accatcgcga
    10621 caaccggttt cggacaggta cggatctgac gctggaagtc cagcacgttc aggtgatgta
    10681 cgccggaatc atctttatag ccgccgtaat caccacgcac tttctggtca ccaccggagc
    10741 agaacgcttt atcgcctgcg ccagtcagaa tgatcacgcc gacgttgtcg tcataacgcg
    10801 catctgccag tgcctggatc atctctttta ccgtcagagg acggaaggca ttgcgcacct
    10861 gcggacgatt aatggtgatt tttgcgatac cgtcggtgga tttttcataa cgaatgtcct
    10921 caaaaccttc ggagcagtcg tgccattcaa ccggtgcgta aagcattgct tcatcaggat
    10981 aaatcatatt gtgtccttta gtcagaaacg caagatctgc gccagacttg cgataacgcc
    11041 agcgggattt tcccgatgcg cgttatgtcc ggcgcgagga atgacatggc agtcggcagc
    11101 cagttccgcc gccaaggcgc ggaatttgct gtcacgttca ccacataaat aataaaacgc
    11161 aaatgtgcgg gcgctaaggt tagcacgtaa atcaggctgg acggcgagag aagtcgcctc
    11221 cagcatggcg gcaagggttg cgccattatt gttactgcgc agcgccacca gctcccggcg
    11281 ttgatcgtca ttgagcgagg caaaaacagg ctgttgatac cagtcggcaa ataccgccgt
    11341 taacggttct gtgcgaaaac gctgcgccca ttggcgatcg gaacgctgac gttccgcgcg
    11401 ttgttcagca ttttgcaacc caggatgccc gccttcgaca acaaccccac aaagccccgg
    11461 catcccctgg caagccgcca tcatcgccac ccgtccccca agcgagtacc ccaccaacca
    11521 gaagttaagg atgttgtaac taaccagggt tttacgcagt aagtcggtga catcatcaaa
    11581 tccatcgacg ctaatcgccg ccgaaccacc gtgacctggg agataaacat acaaccgtga
    11641 gtagtcggca aacgcctcgc ccacttcttg ccattcgtgg caatcgccgg aaaaaccgtg
    11701 caaaaacacc agccagggta aacctggttt gccgtgtttt gcctgcgcgt gcaggatcat
    11761 aaatggctta cctgcgccag aagttgctgg agcgtttgcg caccatcggt gtcgttaacc
    11821 accatttcaa ttaccgtggt ggttggcgtg cgccaggcat cggcaagtgc cgtttcaagt
    11881 tcctgccagt tttgcggacg atgatatttc agctcgaaca tcgcggcggc gtgctcaaaa
    11941 tggacgtttt gcggcatcag atagaaacgc tcgcgctcgc ttttcggcgt tggcagcagc
    12001 gagaaaattt gcccgccgtt gttgttcacc acaattaata tcagcggcgc ggaaacctga
    12061 cgcaataaca ccagcgcgtt gagatcgtaa agtgcggaga gatcgcccac aatcgccagc
    12121 gtcggtttgc cgcttgcccg ctgaacgccg gcggcagtcg agagcagccc gtcgataccg
    12181 ctggccccac ggttgctgta caccgggtaa cctgccggaa gttgcgaaag cgcatcaatc
    12241 agacgtacca ccaggctgtt accgacaaac aattgcccct gttcaggcag atagtcgctg
    12301 atgcgatgcg ccagttgcgc ttcgccaaac gcatcgcgac gggcaataac cgcctgcatt
    12361 gcctgttccg ccaggcgcgg gatttcaacg caccagggct ggcgtttttc tgccggatgc
    12421 agctccagcc agtcggcaat attggcaatt aagcggcgtc cacgatggtg cgccggatca
    12481 agccgccctt cgatgtcatc cacaatccag tactcttcgg gttcacagct tgcctgccat
    12541 tgcaggagcc gtttgcccgt caggctgctt cccagttgca ccacaatttg cgcctgttgc
    12601 agttcgctgg tcgctttggc attgcctaac cagagatcgg cacacggcag cggctgtccg
    12661 gtttgcgaca gcacgtcgcc aatcagcggc cagccaagag tttgcgccca cagtgcaact
    12721 tttttgccct cttccgcact catgcgcccg gcaaccacca cgccgcgttt ttgtcgccag
    12781 aagaaccagt cgcgctgttt ttcactttcc agacgaggcg cttcacgcag ccacggtttg
    12841 tcgtcctgcc accagtcgcc cagccgctgt tgccagctaa gcccggtgtc gtccatttcg
    12901 ccatacagcg gttcggcaaa cgggcagttg atatggactc caccagaatg aagcgtaccg
    12961 agagcatggt cgatggtaga aaccagccaa cgtgcgggga tatcctgggt cgggcgcggc
    13021 aatgaaatac tgtgcgtggg gtgagaggcg aacattcccg gctgacgaat cgcctgattc
    13081 gcgccgcagt caatcagctc cggcgggcga tcggcggtta acaggatcag tttttctccg
    13141 gttaacccag cttcaatcag tgccggatag agatttgcta ccgccgtacc ggaggtcaca
    13201 atcaccgcca ccggctgctt gctgactttc gccagcccca gcgccagatg ccccaaccca
    13261 cgctcatcga aatgggtgtg gtgaatgaat gcggaattct ccgccgccgc taacgttaac
    13321 ggtgtagaac gcgagccagg ggcgatacag atgtgtctga cgccgtgacg cgttaatgct
    13381 tccagaatga ccgccgccca gcgtcggtta aatgcgctta ctgacatgag tttgtccggt
    13441 atcaatattg cggctaagta taaggagcta caaaaataga ttattgatat gaatcggtaa
    13501 tgatgcgact cattactatt ccatctgtaa taaagtacgc agccctgccg ctttgttgtc
    13561 gatttcctgc cactcttgct cggggtcgga accacggaca atgcccgcgc cagcatataa
    13621 tcgcacgaca ttgccgctaa tttttgctga gcgcagggaa acgcagaatt cgctttgctg
    13681 taatgagaga tagcccgcag aaccggcgta ccattctcgg gtgaacggtt cgtgacgggc
    13741 gataaactgt cgcgccagat cgcgcggtaa gccagcaact gctgccgttg gctgcaactg
    13801 atgtaaacag atcacattat ccgctttgtt gagtgaagtc cagatacagc ggcgaagatg
    13861 ctgcacttta cgcagacgca gtacctgcgg cggtaaaaca tccagcgtct gggtatcggc
    13921 ctgtaaccgt tggcagatat cttccaccac cagcatattc tcgcgctggt ttttatcatc
    13981 cgccatcagc cactcaccta actgctgcgc ctgcttatca tcagggtgat ttgctaccgt
    14041 tcccgccagc gcttcagtac gcagcgcttt gtcacgccgc cgccataacc gttccggtga
    14101 agagccaaga aaagcatttt cgccatcaaa ggccatgtaa aaatggtagc aattcagatt
    14161 cagtcgacga ctggcagcca tcatcgccgc cgcgttgacc ggacttgcga aatgcaggtc
    14221 agttgcccga gcgagcacca ctttgtcgag ctcaccttcg gcgatggttt tcgttgccag
    14281 ttcgattaat tgcgtccagc ccgttttgtc cggccagtgt tgttctcgcg tggtggttaa
    14341 atgtaaccca ggcaagggct tgatactcac cagtgtggcg ataaattctc ttgccttaat
    14401 cgcatcgtgc tgaagggagc tttcgctgaa tagcgtcagc cgcagcgtgg ctttaccgcc
    14461 acagcgtcgc cattccaggc gtggtaaaag taaattgccc tgcgacgggt caaacgcatt
    14521 cagcccccaa atgcgtaagt cggcgtgttc cgggtgctgg cgaagaaaac gttgtgcctg
    14581 gtccaacgac gtaaaacggg taatcgcgcc caggacggca gcttcttcat caccattacg
    14641 ttgttgccag tagaattgcg ggtatgtttg ctgactggcc agccagctca aggcatcaaa
    14701 agcgtctttg agagggaaag gaatatcgat aacccgaatg ccgggtgtcg ccggaatctc
    14761 ttgcgacaaa tggcgcagta gattttccag cgccgtagta agtgattgca cgcggacctc
    14821 tccctgttaa aagcctcaca ttatacgggg tactacaaaa aaaatgcagt accccggtgt
    14881 agggaggttt taacggcgtg ccagcaacag tcctagtacc agcccaacgg ccgcgcccac
    14941 accaattcct tgccagggtt tttcgtggac gtagtcatca gcacgataaa ctgcctgctt
    15001 cgcccgatag taataactgt ctgaagcctg gctaacccgt tttttcacat catccagcgc
    15061 tttttctgca cgcgctttca gctcaacata tttctgatcg gcgggatcgc cagaggagcg
    15121 gagcacctct tccagtgttt cactaagcag cgtcaggtcg tcatcgatac gtgtatcacc
    15181 aaactgatta gacattctcg ttctccattt gcgtaaaacc tgtcactaac tatagacaag
    15241 ggttgttgat tacgcctgaa ttacctcgcg tgccatccca atgtgtggga taccatcttc
    15301 ttcatagacc tccgtcacag ggataaaacc gaaactctgg tagaagtttt gcagatgcgc
    15361 ctgcgccccg aggtaaacag gcttatcagg ccagtgatgc gtgcaggtgt cgagggtttt
    15421 actcatcagt tgctgcccca ctttttcgcc acgcaacgct tcactgacaa tcacccgacc
    15481 aataacgacc ggctcaagat catcatcact tttcagaatc ctcgcatatg ccaccagttc
    15541 atcatttttc caaccgagaa tatggcgatt atcccccgtc aaatcatcac catcgatatc
    15601 ctgataaggg caattctgct cgacaacaaa caccgcgcaa cgcagttgta ataaggcata
    15661 aagctgggaa accgataatt cagagtgatg cagatcttac cattcaatca taatgctctc
    15721 cttttagatg tatgccttat actagccgga atattttttg aaacgtgatg aactcatgga
    15781 attaattttt ttaggtactt cagccggtgt gccaacccgc acgcgcaatg tcacggcaat
    15841 attgcttaac ctgcaacatc cgacccagag cggactttgg ttgtttgact gcggtgaagg
    15901 cacccagcat cagctactgc ataccgcctt taaccctggg aaactggaca agatttttat
    15961 cagtcacctt catggcgatc atctctttgg tttacccggc ttgctgtgca gtcgttctat
    16021 gtcaggcatt atccaaccct taacgattta tggtccgcaa ggtatccgtg aatttgtgga
    16081 aaccgcgctg cggattagcg gctcatggac cgattatccg ctggaaattg tcgaaattgg
    16141 cgctggcgaa attctcgatg atggcctgcg caaagtgacc gcttatccga tggaacaccc
    16201 actggaatgt tatggctatc gtattgaaga acatgataaa ccgggtgcat taaatgccca
    16261 ggcattaaaa gctgctggcg tgccgcctgg cccactgttt caggaattaa aagcgggcaa
    16321 aacaatcacg ctggaagatg gaaggcagat taacggcgca gattacttag ctgctccagt
    16381 gccaggtaaa gcgctcgcta ttttcggcga taccggcccc tgcgatgccg cacttgacct
    16441 ggctaaaggt gtcgatgtca tggtgcacga agcgacgctg gatataacca tggaagccaa
    16501 agccaatagt cgcggccata gctctacacg ccaggctgcg acactagccc gtgaggctgg
    16561 agtcggcaag ctaatcatta cccacgtcag ctcgcgctat gatgacaaag gttgtcaaca
    16621 cctgttacgt gaatgcaggt caattttccc ggcgactgaa ctggcgaatg atttcaccgt
    16681 gtttaacgtt taactc
//
LOCUS       DADMKF010000084        16525 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.085, whole genome shotgun sequence.
ACCESSION   DADMKF010000084 DADMKF010000000
VERSION     DADMKF010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16525
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.085"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(94..246)
                     /locus_tag="JNC24_002529"
     CDS             complement(94..246)
                     /locus_tag="JNC24_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HAY9159250.1"
                     /translation="MLTKYALVAIIVLCFTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            complement(511..1245)
                     /gene="cysH"
                     /locus_tag="JNC24_002530"
     CDS             complement(511..1245)
                     /gene="cysH"
                     /locus_tag="JNC24_002530"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HAY9159251.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIRPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMSEEETRFFGLKRECGLHEG"
     gene            complement(1320..3032)
                     /gene="cysI"
                     /locus_tag="JNC24_002531"
     CDS             complement(1320..3032)
                     /gene="cysI"
                     /locus_tag="JNC24_002531"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HAY9159252.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIITGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            complement(3032..4831)
                     /gene="cysJ"
                     /locus_tag="JNC24_002532"
     CDS             complement(3032..4831)
                     /gene="cysJ"
                     /locus_tag="JNC24_002532"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HAY9159253.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPAALAATPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDADVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLKGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTPIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     PQTPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            5147..5512
                     /gene="queD"
                     /locus_tag="JNC24_002533"
     CDS             5147..5512
                     /gene="queD"
                     /locus_tag="JNC24_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HAY9159254.1"
                     /translation="MMSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGE
                     VDPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPL
                     LSAVMVKETCTAGCIYRGE"
     gene            5590..6861
                     /locus_tag="JNC24_002534"
     CDS             5590..6861
                     /locus_tag="JNC24_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAY9159255.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARAGLSVLLLERAEIPGSKNL
                     SGGRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGVTTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLTRFKAHPAVRPLIKNTESLEYGAHLEP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFALYHHNVERSLLWDVLQRYQHVPALLQRPGWYRAWPALMQDISRDLWDQGDKP
                     VPPLRQLFWRHLRRHGLWHLAGDVIRSLRCL"
     gene            6852..7112
                     /locus_tag="JNC24_002535"
     CDS             6852..7112
                     /locus_tag="JNC24_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HAY9159256.1"
                     /translation="MSVAHNLWRVADAPHIVPADSVERQMVQRLINACPAGLFSLTPE
                     GDLRVDYHGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            7129..7704
                     /locus_tag="JNC24_002536"
     CDS             7129..7704
                     /locus_tag="JNC24_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HAY9159257.1"
                     /translation="MPLLHLLRQNPVIAAVKDNASLQLAIDSECQFISVLYGNICTIS
                     NIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEADGIISTKASMLKAARAEGF
                     FCIHRLFIVDSISFHNIDKQVAQSNPDCIEILPGGMPKVLGWVTEKIRQPLIAGGLVC
                     DEEDARNAINAGVVALSTTNTGVWTLAKKLL"
     gene            complement(7852..8745)
                     /locus_tag="JNC24_002537"
     CDS             complement(7852..8745)
                     /locus_tag="JNC24_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HAY9159258.1"
                     /translation="MAGLFTPKDAAMNIAIVTINQENAAIASWLAAQDFSGCTLAHWQ
                     IEPQPVVAEQVLDALVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLD
                     IPTVSVRKSHWGNALTATLQTEKRPLCLSLARQAGAAKNATLPSGMQQLNIVPGALPD
                     WLVSTEDLKNVTRDPLAEARRVLVVGQGGEADNQEIAMLAEKLGAEVGYSRARVMNGG
                     VDAEKVIGISGHLLAPEVCIVVGASGAAALMAGVRNSKFVVAINHDASAAVFSQADVG
                     VVDDWKVVLEALVTNIHADCQ"
     gene            complement(8709..9488)
                     /locus_tag="JNC24_002538"
     CDS             complement(8709..9488)
                     /locus_tag="JNC24_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HAY9159259.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGKSGPDISLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQHQNPLDLIITGCQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDALFI
                     TLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPA
                     MQCLQLARAEQRRGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            complement(9466..10875)
                     /locus_tag="JNC24_002539"
     CDS             complement(9466..10875)
                     /locus_tag="JNC24_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY9159260.1"
                     /translation="MTGRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     AALTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMIGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFLLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGILLGAFSVVWTVGYVLASIAGHHFISENPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLRQGRFAEAHAIVHRYFGPHVLLGDEVVTATHKHIKTLFSSRYWRRTAFN
                     SVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQVTLLLLATVLLVDFVVTWLWAPETKA
                     LPLVAAGNVGGANEHSVSV"
     gene            complement(10897..12351)
                     /locus_tag="JNC24_002540"
     CDS             complement(10897..12351)
                     /locus_tag="JNC24_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HAY9159261.1"
                     /translation="MSLSRAAIVDQLKEIVGADRVITDETVLKKNSIDRFRKFPDIHG
                     IYTLPIPAAVVKLGSTEQVSRVLNFMNAHKINGVPRTGASATEGGLETVVENSVVLDG
                     SAMNQIINIDIENMQATAQCGVPLEVLENALREKGYTTGHSPQSRPLAQMGGLVATRS
                     IGQFSTLYGAIEDMVVGLEAVLADGTVTRIKNVPRRAAGPDIRHIIIGNEGALCYITE
                     VTVKIFKFTPENNLFYGYILEDMKTDFNILREVMVEGYRPSIARLYDAEDGTQHFTHF
                     ADGKCVLIFMAEGNPRIAKATGEGIAEIVARYPQCQRVDSKLIETWFNNLNWGPDKVA
                     AERVQILKTGNMGFTTEVSGCWSCIHEIYESVINRIRTEFPHADDITMLGGHSSHSYQ
                     NGTNMYFVYDYNVVDCKPEEEIDKYHNPLNKIICEETIRLGGSMVHHHGIGKHRVHWS
                     KLEHGSAWALLEGLKKQFDPNGIMNTGTIYPIEK"
     gene            complement(12421..13237)
                     /locus_tag="JNC24_002541"
                     /pseudo
     CDS             complement(12421..13237)
                     /locus_tag="JNC24_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417254.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            13555..14832
                     /locus_tag="JNC24_002542"
     CDS             13555..14832
                     /locus_tag="JNC24_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY9159262.1"
                     /translation="MQHNSYRRWITLAIISFSGGVSFDLAYLRYIFQIPMAKFMGFSN
                     TEIGLIMSTFGIAAIILYAPSGVIADKFSHRKMITSAMIITGLLGLLMATYPPLWVML
                     CIQVAFAITTILMLWSVSIKAASLLGDHSEQGKIMGWMEGLRGVGVMSLAVFTMWVFS
                     RFAPDDSASLKTVIIIYSVVYILLGILCWFFVSDNNNLRSANNEEKQSFQLSDILAVL
                     RISTTWYCSMVIFGVFTIYAILSYSTNYLTEMYGMSLVAASYMGIVINKIFRALCGPL
                     GGIITTYSKVKSPTRVIQILSIIGLLALTALLVTNSNPQSVAMGIGLILLLGFTCYAS
                     RGLYWACPGEARTPSYIMGTTVGICSVIGFLPDVFVYPIIGHWQDTLPAAEAYRNMWL
                     MGMAALGMVIVFTFLLFQKIRTADSAPAIASSK"
     gene            14859..16344
                     /locus_tag="JNC24_002543"
                     /pseudo
     CDS             14859..16344
                     /locus_tag="JNC24_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311659.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
BASE COUNT         3923 a         4355 c         4163 g         4084 t
ORIGIN      
        1 cataatattc acataaataa tataaattaa aaaagggtgc aggagaccaa accatcacaa
       61 ttcagtacgc ggggttttac ccccgcgtgg tggctacttc ttcgattcgt aagcgagaac
      121 tgccttaaac tccataccac gttctctgat actcaactca cacaacgagt cgcctaccat
      181 cagcgtgaat cccagtactg taaaacacag cactatgatt gccacaaggg catattttgt
      241 cagcatgtct tgcctccctg caaagaagag actaacattc gaattgtttg ggttcgaacg
      301 ctggcctcag gttgatagaa atatcgcctg gggcttttgt ccatctggaa cctcgcgaat
      361 gcttaacgcc agacagcctc aagcacccga cgccattcta tacctgataa ttcttccagc
      421 ggcgttcacg tttaaaatca ggaatactgc tcgcaaaata aaaacgcccc catcatttct
      481 gacagaggcg tttaatttat ccggcaatat ttacccttcg tgcaatccac attccctttt
      541 taagccaaag aaacgtgttt cttcttccga catgccaggt tcccatttac gggttgtatg
      601 ggtatcaccg accgataaat atccttcatc ccataatggg tgatatttca ggccatgttt
      661 ttgcaggtac tgataaatag ttcggttatc ccagtcgata atcggcagca ctttaaatac
      721 gccacgctga attgccagca ccggtaaatt ggcgcgactg ccggattgtt cacggcgcag
      781 gccagcaaac caggtttgcg cgttaagttc tttcagagcc cggttcatcg gttcgacttt
      841 gttgatgtca ttgtactttt caatgccttc aacgccctgc tcccacagtt ttccgtagcg
      901 tgcttcctgc caggctgcgc tttcggtagc acggtacact ttcaggttga gcttgagttt
      961 gtccgttaac tcgtcaataa agcggtaggt ttccgggaac aagtaacccg tatcggtgag
     1021 gatcaccgga atatccgggc gaatttgatt caccagatgc aggctcaccg ccgcctgaat
     1081 gccaaagctg gaagaaagta catattcacc gggcagatta tccagcgccc aggctacgcg
     1141 gccttcagcg tccagttttt ccagttcggc gttagtttcc gccagcgcca gaatacgatc
     1201 taccttcggc agttcgttca gggcgtttag atcgagtttg gacatagatt cctcactgtt
     1261 tgccttgcct gatgcgacgc tcacgcgttc ttatcaggcc tacaagaccg ggctgatggt
     1321 taatcccaca aatcgcgcgc cggatcgagc accgggcgaa tgatgcccgc acgcaccgta
     1381 aagtcgccga agccttcacc cgcttcgcgc tctttcgccc agcgccctat cagttcatca
     1441 agcgacgcca ggatttccgg ctcggtgatg ttttctttat acatccgtgg gatacgtgtc
     1501 ccaatgcggt tgccgccaag atgcaggttg tagcgacccg gcgctttacc caccaggccc
     1561 acttccgcca gcatcgcgcg accacaaccg ttcgggcagc ctgttacacg catcacgata
     1621 tgctcatcgc tgacaccatg tttcgccatt aaattatcga tgttgtcgat aaaagacggc
     1681 aggaaacgct ctgcttccgc catcgccagc gggcaagtcg ggaatgacac gcaggccatc
     1741 gagttttcac gctgcggcgt gacggcattc attaacccgc tctctttggc gatcttctcg
     1801 atcttcgctt tctcgctttc cggtacaccg gtgatgatca gattctggtt cgccgtaatg
     1861 cggaaatcgc ctttgtggat cttcgcgatc tccagcaggc cggttttcag cggacgcccc
     1921 ggataatcaa ggatgcgacc attttcgata aacagcgtca ggtgccagtt atcatcaatg
     1981 cccttaaccc agccaatacg atcgcctcgt ccggtgaact catatggacg gatcggttca
     2041 aatttgatcc ccgcgcgacg ctccacttcc gctttaaacg tctcaacccc cacgcgctcc
     2101 agcgtgtatt tggttttggc atttttacga tcggttcggt taccccagtc acgctgagtt
     2161 gtcacgacgg cttcggccac cgccagcgta tgctccagcg gcagatagcc aaactcgctc
     2221 gccgtgcggg cgtaggtttt cttgttgccg tgttcgatag aaagcccacc gcccaccagc
     2281 aggttgaagc ctaccagctt gccgttttcg gcgattgcca cgaagttcat gtcgttggca
     2341 tgcagatcga tatcgttctg cggcgggatc actaccgtgg ttttgaactt acgcggcagg
     2401 taggtctgac caaggatcgg ttcttcatca gtagtggcga ctttttcctg gtcgagccag
     2461 atctccgcat acgcgcgggt acgaggcaac agatgctcag aaatcttctt cgcccactcg
     2521 tacgcttccg cgtgcagctg cgactcgtaa gggttcgagg tgcagagtac gttacggttc
     2581 atgtcgttag ctgtcgccag cgcatcaaga ccgaccgagt gcagcatctg gtgcaccggt
     2641 ttgacgttct ttttcagaat gccgtggaac tgaaacgtct ggcggttggt caggcgaatg
     2701 ctgccataga tggtgttttc accggcaaat ttgtcgatcg cctgccactg tttagtggta
     2761 atcaccccac ccggcagacg acagcgaagc agcatcgcgt ggcgcggctc cagcttctgt
     2821 tcagcacgtt cggcacggat gtcgcggtca tcctgctgat acatgccgtg gaagcggatc
     2881 agcaggaagt tgtcgccctt aaagccgccg gtcagaccgt cgtttaaatc ttccgcaatg
     2941 gtgccgcgca ggtagttgct ttcaagcttc atgcgctcgg cgtctgtcag ttttccttcg
     3001 accactaaag gccctggatg tttttcgctc attagtagac atctcgctga taacggcgct
     3061 ctacgcgcag ctcacttaaa aattcatccg ccgcttcggt gtccatgcca ccaaattcgg
     3121 caatcacttc cagaagtgcc tgctcaacgt ctttcgccat gcgattagcg tcgccgcaga
     3181 cataaatgtg ggcaccgtca ttgatccagc gccacagctc cgcgccctgt tcgcgcagtt
     3241 tgtcttgtac ataaactttt tctttttggt cgcgcgacca ggcaagatcg atacgcgtca
     3301 gcacgccctc tttgacgtag cgctgccact ccacctggta gaggaaatct tccgtaaagt
     3361 gcgggttgcc aaagaacagc cagtttttac ccggcgcttc gtcggcggcg cgttgctgca
     3421 taaaggcacg gaacggcgcg atgccggtac cggggccaat cataatcact ggcgtttgcg
     3481 ggttagcggg caggcggaaa ttgtcgttat gttcgataaa tacgcggact tcgccctctt
     3541 cttccacgcg atccgcgagg aagctggagg caccaccggc acgggcgcgg ccttccacgt
     3601 cgtaacgcac cacaccaacg gtgacgtgta cttcgttctc gacttccgcc tgcgaggagg
     3661 cgatggaata caggcgcggg gtcagcgggc gcagcaggtt aatcagcgct tcggcatcca
     3721 gttgcgccgg agagaaacgc accatgtcga caatcggcgt ggtcgcggcg taatgctgta
     3781 acttcgcttt atcgcccacc agcggcagca gcgtttcgct gcgggtaagc gtggcatagt
     3841 tctcaacaat gttggcggtg ttgacggtca gttcgaagtg ccactgtagc gcttcgttca
     3901 gaggcaacgt tttgccctcg acggtgacag gttcatcgcc tttcagccac agcagttcga
     3961 caagttcttt caccagtgcc gggtcgttct gataccagac acccagcgcg tcacccggct
     4021 ggtaacgcag gcctgaatcg cctaagtcaa tttcgatatg acgaacgtct ttttcagagt
     4081 tacgcccggt aattttctgg ttaaccgaaa ggctcgccac cagcggcgcg tctttgctgt
     4141 acgggctggt gtggatttca tttaccgcgc cagtagcgac ggattgcgaa ggtgccgcga
     4201 caggcgcacg cgatttaagc gcatcaacca cgcgggcgcg ccactcgctg gcagcagcct
     4261 ggtattcaac atcggcatcg acacggtcga gcaggcgttc accacccagt tccgccagct
     4321 tgctgtcgaa atctttcccg gactggcaga aaaattcata agagctatcg ccgaggctaa
     4381 acacggcaaa cgcggtgttt tccagctttg gcgctttttt ggagaacagg aacttatgca
     4441 gcgcgacggc ttcttccggc ggttcccctt ccccttgcgt tgacgtcact acgatgagca
     4501 gtttttcgct ggcgatttgt ttgaatttat agtcgcccgc gttcaccagc ttaacgttca
     4561 gttttgctgc taataaatca tcgcgtaatg cttcagcaac ccggcgcgca ttgccggttt
     4621 gcgaggcgga gataatagtt atacccggca tttctgcggc tggcgctggc gtcgctgcaa
     4681 gcgcagcagg ctgctgattg agtacgcccc agaaatagcc agaaacccag gcaagctggg
     4741 taggcgttaa atcggtcgtg gccgcctgaa ggcgtgccag ttgctccggg ttcaacggaa
     4801 gcaacgcgga aggtgggacc tgtgtcgtca tgcgtcgtta tgttccagta agcaaagctg
     4861 tttctgcgcc ctgtcagcgc ccataaaaca gaagagaaga taaggttaac ggggtgaacg
     4921 gtgtggatta aagacgggat agcgataact aataaccaaa tcgactaacc tgttttagca
     4981 atagtcttta acaacaaaat agattaacca acccaatgaa aaacaaatga atttagccaa
     5041 tcattaagat aaatcagcga ttttgcgcaa caaagtcgtt ttagataatg cgaaaaaaca
     5101 gcctttccgg tactctacgg cggttttatc gtcctgtaga gaaattatga tgtccaccac
     5161 gttatttaaa gatttcacct tcgaagccgc tcaccgctta ccacacgtcc cggaagggca
     5221 taaatgtggt cgcctgcacg ggcattcctt tatggtgcga ctggaaatta ccggggaagt
     5281 cgatccgcat acgggctgga ttatcgattt cgctgaactg aaagcggcgt ttaagccaac
     5341 ttatgagcgc ctcgatcacc attatctcaa tgatattcca ggtctggaaa acccaaccag
     5401 cgaagtttta gcaaaatgga tttgggatca ggttaaaccc gttgtgccgc tgttaagtgc
     5461 ggtgatggta aaagaaacct gcaccgcagg gtgtatctat cgcggcgaat gataagagtg
     5521 tgtcggcggt caatttccct taagtaacgc tatgttaggg tgttgtgttc tggatatctg
     5581 gggcatgaca tggaagacga ctgcgacatt attattattg gtgccggtat tgcaggcacc
     5641 gcttgcgcgt tacgctgcgc gcgagcgggt ttatccgttt tgttactgga acgcgctgaa
     5701 atccccggca gcaaaaatct ttccggcggg cggttatata cccatgcact cgcggaactc
     5761 ctcccgcaat ttcatctgac cgcgcctctt gaacgacgca tcactcacga aagcctttcc
     5821 ctgttaacgc ccgatggcgt aacgacgttt tccagcttac agcccggcgg tgaatcctgg
     5881 agtgtattac gtgcacggtt cgatccgtgg ctggttgccg aagccgaaaa agaaggtgtc
     5941 gaatgcatcc ccggagcgac ggtggatgca ctgtatgaag aaaacggcag agtctgtggc
     6001 gttatttgtg gtgacgatat tctccgcgcc cgttatgtgg tgctggcaga aggtgccaac
     6061 agcgtcctgg ctgaacgtca cgggttagtg actcgtcctg ctggcgaagc gatggcgttg
     6121 gggatcaaag aagtgctgtc gctggaaaca tccgctattg aagaacgttt tcatctggag
     6181 aataacgaag gcgcagcgtt gctgttcagc ggcggaatct gtgatgactt acccggcggc
     6241 gcatttcttt atactaatca acaaacgctc tcgttaggga ttgtttgccc gctctcttcc
     6301 cttacgcaaa gtcgtgttcc ggcaagcgag ctgctgactc gctttaaagc gcatccggca
     6361 gtgcgcccgc ttatcaaaaa cacggaatca ctggagtatg gtgcgcatct ggagccagaa
     6421 ggtggcttgc acagtatgcc ggtgcaatac gccggtaacg gctggctgct ggtgggcgat
     6481 gcgttgcgca gttgcgtcaa taccggaatt tccgtgcgcg gcatggatat ggcgctgact
     6541 ggcgcacagg cggcggcaca aacgctaata agcgcctgcc agcaccgcga gccgcaaaat
     6601 ctgtttgcgc tttatcatca caacgtagag cgcagcctgc tatgggatgt tctacaacgt
     6661 tatcagcatg ttccggcgct tttgcaacgc cctggctggt atcgggcgtg gcctgcgtta
     6721 atgcaggata tttcccgcga tttatgggat cagggtgata aacctgttcc accgctgcgc
     6781 cagttattct ggcgtcattt acgtcgtcat ggcctgtggc atctggcggg cgatgttatc
     6841 aggagtctgc gatgtctgta gcccataatc tctggcgcgt tgctgatgcg ccgcacattg
     6901 ttccggctga ctccgttgag cgtcagatgg tacaacggtt gattaacgcc tgtccggcag
     6961 gtcttttttc gctcacaccg gaaggtgact tacgcgttga ttatcacggc tgcctggagt
     7021 gtggcacctg ccgtttgctg tgcgacgaat ccacactaca acagtggcgc tatccgcctt
     7081 ccggattcgg catcacctac cgctttggat aaataataag gataatttat gcccctctta
     7141 cacctactcc gccagaatcc ggtgattgct gccgttaaag ataatgccag cctgcaactg
     7201 gctatcgatt ctgaatgcca gtttatttcc gtgttgtacg gcaatatctg caccatcagt
     7261 aatatcgtta aaaagattaa gaacgccggg aaatatgctt ttattcatgt tgatttgctg
     7321 gaaggcgcgt caaataaaga agtcgttatt cagtttttga aactggtgac cgaggcggac
     7381 ggcattatca gtaccaaagc ctcaatgttg aaagcggcaa gagcggaagg ttttttctgt
     7441 attcatcgcc tgtttattgt tgattcaatt tcgtttcaca acattgataa gcaagttgcg
     7501 caatcgaatc cggattgtat tgagatcctg ccaggcggta tgcccaaagt gctgggctgg
     7561 gtgacagaga aaatccgcca accgctgatt gccggtgggc tggtgtgcga tgaagaagat
     7621 gcgcgtaatg cgattaacgc gggtgtcgtg gcgctttcca ccacgaatac cggggtctgg
     7681 acgttagcga aaaaattact ttgacgggat aatcgtaacc aattgaattt ggtttgattt
     7741 gtaggccgca cgccacatcc gacattcagc gcctgatgcg acgcttgacg cgtcttatca
     7801 ggcctacaag tccccaggcc agaaccgtag gcaggatcat ccggcaacac gttattggca
     7861 atctgcgtga atgtttgtca ccagtgcctc cagcaccact ttccagtcat caaccacgcc
     7921 aacatctgcc tgtgaaaata ccgcagcgct ggcgtcatga ttaatcgcca ccacaaactt
     7981 gctattgcgt acgcccgcca ttaacgctgc tgcgccagaa gcgccaacaa caatacacac
     8041 ctcaggtgcc agcagatgtc cggaaatacc aatcactttt tcagcatcaa cgccaccgtt
     8101 catcactcgc gcccgactgt agcccacttc ggctcccagc ttttctgcca gcatagcgat
     8161 ttcctggtta tctgcctctc cgccctgccc caccaccagc acccgtctgg cctcagctaa
     8221 tgggtcgcga gtaacatttt tcagatcctc tgtgctaacc agccagtccg gaagcgcacc
     8281 cgggacaatg ttcagttgtt gcattccaga gggcaacgtg gcgtttttcg ctgctcccgc
     8341 ctgacgcgcc agggaaaggc atagcgggcg tttttctgtt tgcagcgtcg ccgttagcgc
     8401 atttccccag tgggatttgc gcacgcttac ggttggaata tctaacgaag tcacctggca
     8461 gatgcttgca ccatgtaaac gccaggccag ccgggtcgac agttcatcgc caaacgttcc
     8521 cggcggaaag agaacaacgt ccgcaggcgt tcgctgccac tgttccacca acgcatccag
     8581 cacctgttcc gccactacag gctgaggttc aatctgccag tgcgccaggg tacaaccgct
     8641 gaaatcctgc gccgccagcc agcttgctat tgcggcattc tcctgattta ttgtcacaat
     8701 tgcgatattc atggctgcat cctttggcgt aaatagtcct gccatagctt ttgagctttt
     8761 tctgctaccg tttgcccatc aatcagcgtc gctccgcgtc gttgctctgc tcttgccagt
     8821 tgcagacact gcatcgctgg catttcggca gcaacagttt tacggataat ttctgctttc
     8881 cccgcagcca ttcgctggcg cattcccggt actggcaagg ccacttcacc gcactgacgc
     8941 acggcaatca ccgcaggcaa gcgaacccgg caacaacgca acccatgttc agtacgctgt
     9001 tcgagggtga taaacagcgc gtcgagagtg aaacgctcca cctgggtgaa gcagggccag
     9061 cccagcattt ccgccagcaa aaatggcgtt tgcccattct gcccttcgct gctttggcag
     9121 ccggtaatta tcagatccag cggattctga tgctgccatt cggcaatatg gcgagcgaca
     9181 aattccggcg caaagcgcag atctgccgcc gtctccagca atacagcttc ctcaaaccca
     9241 agagccatga gatagcgcag ccagtgcaat gcccgttcat cccccatgct caacgcggtt
     9301 aaagacatcg gcgtaccgtt ttttctctgc gccaacagca gcgcggcagc ggcctgttca
     9361 tcagcaccga gtaaacttcg cagtagcgaa atatccggtc cgcttttacc ctgagccgcc
     9421 gcctgccact ctttttccgc cagcattccg gcatccggtt cggctttaaa cgctaacaga
     9481 atgttcattc gcacctccta catttcccgc cgccaccagc gggagggctt tagtttctgg
     9541 tgcccatagc caggtcacaa caaaatcaac caacaacact gtcgccagga gcaataaggt
     9601 gacttgcatt ccccactgcg ccagcaccca cggcagcagg ccagtactta ccgccgcgcc
     9661 aagtcgactc atggcggtgg caaaaccgac gcccagcgag cgaatgtcag taggaaaact
     9721 ttccgcaggc aaaatgccca ccagattact gactgccgaa atggtggtgc tgaagagaac
     9781 aaaaagcagc agcgttaatg aactgccgga aggcaaacag gccatcacta ccagcgttgc
     9841 cgccagcagc aaaaaacttc ccagcaaaaa tttgcgatgt gccagcaggt gcgtcagaac
     9901 taatcccagc agcgcgccca caattaacaa cgcattaagc atcaggctgg cagtcagcgc
     9961 atcttccaga ccaatagtct gggcgatagt tggcagccag gtataaatca caaaccatgg
    10021 gattacgagg cagacaaaga agacgctgtt aaacgccgtg cgccgccagt aacgcgaaga
    10081 gaacaaggtt ttgatgtgtt tatgggtcgc cgttaccact tcatcgccca gtaaaacatg
    10141 gggaccaaaa tagcgatgca cgatagcgtg agcttctgca aaacgcccct ggcgcagtag
    10201 ccagcgtggt gattctggcg ttccccagcg taataacgta atcaacaacg cgggcagagc
    10261 tgccgatgcc agtagccagc gccaggcctc cgggttttcg gaaataaagt gatgtccggc
    10321 aatacttgcc agcacatagc ctacggtcca caccacgctg aatgcgccca gcaaaatacc
    10381 gcgatggcgg cgcggggaaa attcagccag caaggtgtga cctactgaat aatcgcctcc
    10441 cagaccaatg ccaatcaaaa tgcgcagtcc aataagatgc tctggcgtgg tggcaaaaaa
    10501 ttgtaaaaac gaagcaagcg taatcagcaa aaagctgaag gtgaagattt tttgccgacc
    10561 aatatggtcg gagatccacc caagaaccag gctgccaagg aacaaaccaa ggagcgccga
    10621 gccgccgatc attcccgcca taaacggcgt cagttgcatg gcgggcgtaa gctgaataat
    10681 ggcgtaacca atgacgccga gaacataacc gtcggtcagg tgtgcgccga aagtgagcgc
    10741 agcaatgcgg cagtgaaaac ggttaagcgg taaatcatcc attcgcaccg gtgaagtgtt
    10801 catctttacc ccttcattgt ccggtaataa gaacggcttc tcgcctgaga agccgaaaag
    10861 gcaacgcccc gtcatcgcgg aacgctgcct gacacattat ttttcaatcg gatagatagt
    10921 acccgtattc ataatgccat taggatcgaa ctgctttttc agcccttcca gcaacgccca
    10981 cgcgctgccg tgttccagtt tgctccagtg aacgcgatgt ttaccgatac cgtggtggtg
    11041 caccatcgaa ccgccgaggc gaatggtttc ttcacagatg atcttgttga gcggattgtg
    11101 gtacttgtcg atttcctctt ccggcttaca gtcaacgacg ttgtaatcgt agacgaagta
    11161 catgttggtg ccgttctgat agctatgaga ggaatgaccg cccagcatgg tgatgtcgtc
    11221 ggcgtgcggg aactcagtac gaatacggtt aataacgctt tcgtagattt cgtggatgca
    11281 gctccagcag ccggacactt cagtggtaaa gcccatgttg ccggttttga ggatctgcac
    11341 acgttcggca gccactttat ccggtcccca gttcaggttg ttaaaccagg tttcgatcag
    11401 cttgctgtcc acgcgctggc attgcgggta gcgggcaacg atttccgcaa tcccttcgcc
    11461 cgtcgccttc gcaatgcgag ggttaccttc agccataaag atcagcacgc attttccgtc
    11521 tgcaaaatgg gtgaagtgtt gggtgccatc ttcagcgtca tacaaacgag cgatcgacgg
    11581 acgataccct tccaccatga cttcacgcag gatgttgaag tcggttttca tgtcttccag
    11641 gatatagccg tagaagaggt tgttttccgg ggtgaattta aagattttca ctgttacttc
    11701 agtgatatag caaagtgcac cttcgttacc gatgatgatg tggcgaatgt ccgggccagc
    11761 cgcgcggcgt ggcacgtttt taatgcgtgt gacggtgcca tcagccaata ctgcttccag
    11821 accaacgacc atatcttcga ttgcgccgta gagtgtggag aactgcccga tactgcgggt
    11881 tgctaccaag ccgcccatct gcgccagcgg ccttgactgc ggagaatgcc ccgtggtgta
    11941 acctttttca cgcaacgcat tttccagcac ctccagcgga acgccacatt gcgccgtcgc
    12001 ctgcatattc tcaatatcaa tattaatgat ttgattcatg gcggagccgt caagcaccac
    12061 cgagttttcc acaacggttt ccagcccacc ttcagtggcg gaagcgccgg tacgcggcac
    12121 accgttaatt ttgtgcgcat tcataaaatt cagcacacgg gatacttgct ctgtggaacc
    12181 gagttttacg accgctgccg gaatcggcaa agtataaatg ccatgaatat ccggaaattt
    12241 acgaaaacgg tcaatactgt tcttctttaa tactgtttca tcggtaatta cgcgatctgc
    12301 accaacaatt tcctttagtt ggtcgacaat cgctgcgcga gataaagaca tagtaattcc
    12361 ttcctgataa taaaaatgga ataatcatta ataagagaaa acagaaacca ccgttatgga
    12421 ttagcgcact aaataaccac cattgaccac taataaatgc ccattgacat aatttgaagc
    12481 cggacttgcg aggaatacag ctgcgcccat taaatcctga gtatcgcccc aacggtttgc
    12541 cggaatatga tcaagaacgc gctgattggt ttctggatta ctgcgtgtcg ccagcgtaat
    12601 atctgttgca taatagccag gagcaatacc atttacctga atattatatt gacctagttc
    12661 atcacaataa gctttggtga atccggcaag agcatgttta gtggcagaat atgcaggtga
    12721 ccattgtcca cctaagtaag agaacaatga acagatatta ataattttgc cgcttttctg
    12781 cgggatcata atttttgcag cttcatagct taactcgaat gcggcggtca ggttcacatc
    12841 aatcatcgga tcccagtcgg cacgaccgaa gtccagcacc ttattcagct tacaaatacc
    12901 ggcattgttt accagaatat caactgtacc gaaacgctca cagcaagcag cgataatctt
    12961 ctgcggcgcg ccttctgcgg tgatatccac ctgcatgaag tccacctcaa caccctgttt
    13021 ttcaatcatt tcctttgttt cgccgttatc tttcaatcat ttcctttgtt tcgccgttat
    13081 ctttgacgaa actaggaata aagatatttg cgccagcttt ggccaacgcc atggcaaatg
    13141 cctggcctaa accgctattc ccaccggtaa caattgcggt tttacctttc agtgagaaaa
    13201 aatccattga gaacgcattg agagattcga ttgacatagt tagctccaat ttccgtaagg
    13261 caaaaaaaag aaaggtaact tcccccgcga gagggaagtt acctttctca ttatctctgg
    13321 taactggaga taatttttag cataacggca taactgctaa tgtgatcgta atcacagtgt
    13381 gatattcgta ttgtcaaata gttttaaaaa gcagaagcgt gattactctc acacaacacc
    13441 tctgctttcc atgttatttc ttattgcaat tactagagac ctgagaaaag taatccacct
    13501 gaaagggcgg cttacttttc tctttttttt gtctattaca aatgggaata cgatatgcaa
    13561 cacaactcat atcgccgttg gataaccctc gcgataatta gttttagcgg cggcgttagt
    13621 tttgacctgg cttatttacg ttatattttt caaattccca tggcgaaatt tatgggattc
    13681 agcaataccg agataggttt aataatgagt acctttggta ttgcggctat tattctttat
    13741 gcccccagcg gcgttattgc cgataaattt tcacaccgca aaatgattac ttccgcgatg
    13801 atcattaccg gattactggg tctgttaatg gcaacgtatc caccgctgtg ggtaatgctc
    13861 tgtattcagg tcgcctttgc gataacgacg attttaatgc tgtggtcggt gtcgattaaa
    13921 gccgcctcgt tgctgggcga tcatagtgag caagggaaaa ttatgggctg gatggaaggg
    13981 ctgcgcggcg tcggtgtaat gtcgctggcg gtgtttacca tgtgggtctt ttctcgcttt
    14041 gcaccggatg acagcgccag cctgaaaaca gtcattatca tctacagtgt ggtttacatc
    14101 ttgttgggga ttctgtgctg gttttttgtt agcgataaca acaacctgcg cagtgccaat
    14161 aacgaagaaa aacagtcatt ccagcttagc gacatccttg ccgttttgcg tatcagcacc
    14221 acctggtatt gcagcatggt gatttttggt gtcttcacaa tctacgccat tctgagttac
    14281 tccaccaact atctgaccga aatgtacggc atgtcgctgg tggcagcgag ctacatgggg
    14341 attgtgatca acaaaatctt ccgcgcgctg tgcggcccac ttggaggcat tatcaccacc
    14401 tacagtaaag tgaaatcccc tacccgcgtg attcaaatcc tttccatcat cggtttgctg
    14461 gcgttgacag ccctgctcgt cacgaactct aacccccaat cggtcgcgat ggggattggc
    14521 ctgattttac tgctgggatt cacctgttac gcctcacgcg ggctgtactg ggcctgcccc
    14581 ggcgaagcga gaacaccgtc ttacattatg ggcaccacgg taggtatttg ttcggtgatt
    14641 ggattcctgc cggatgtctt cgtttacccg attatcggcc actggcaaga caccctgccc
    14701 gctgcagaag cctaccgcaa tatgtggctg atgggcatgg cggcgctcgg catggtgatt
    14761 gtctttacct ttttgctgtt ccaaaaaatt cgtactgctg atagcgcccc cgcaatagct
    14821 agcagcaagt aagccaggaa aattacagga gaaaaatcat gtcgaaaaaa tacatcatag
    14881 ggattgatgg cggaagtcag agcacaaaag tggtgatgta cgatctggaa ggtaatgtgg
    14941 tttgcgaagg taaaggctta ttacagccga tgcacacgcc tgatgccgat actgcagaac
    15001 atcctgacga cgatttatgg gcatcattat gttttgcagg tcacgatttg atgagccagt
    15061 ttgccgggaa taaagaagat attgttggca tcggcctggg ctccatccgt tgctgccgtg
    15121 cgttattgaa agccgacggc acgccagctg cgccgttgat tagctggcag gatgcacgcg
    15181 ttacacgccc ttacgaacat acgaatcctg acgtggcgta tgtaacctct ttttcgggtt
    15241 atctgacgca tcgcttaacc ggcgaattta aagataatat cgccaactat tttggtcagt
    15301 ggccggtgga ttataagacc tgggcatgga gcgaagatgc cgcagtcatg gagaagttta
    15361 atatcccccg tcatatgctg tttgatgtgc aaatgccagg cactgttctt gggcatatca
    15421 cacctcaagc cgcgctggcg acgcatttcc cggcaggact gccggttgtt tgtaccacca
    15481 gtgataaacc ggtagaagct ctgggagccg gattactgga tgatgaaact gcggtaattt
    15541 ctcttggcac ttacatcgca ttgatgatga acggcaaagc cttgccgaaa gatccggtgg
    15601 cgtactggcc gattatgtct tctattccgc aaacattgct gtatgaaggt tacggtattc
    15661 gcaaaggcat gtggacggtg agctggctgc gcgacatgtt aggcgagtcg ttaattcagg
    15721 atgccagggc gcaggatctt tcaccggaag atttactcaa caagaaagcc tctagtgtgc
    15781 cacctggctg taatgggctg atgacggtgc tggactggct gaccaatccg tgggaaccgt
    15841 acaaacgcgg gattatgatc ggctttgatt ccagcatgga ttacgcatgg atatatcgtt
    15901 cgatattgga aagcgtggcg ctgacgctga agaacaatta cgacaatatg tgtaatgaaa
    15961 tgaatcactt tgcgaaacat gtaatcatta ctggcggcgg ttcgaacagg atctgtttat
    16021 gcagattttt gccgacgtgt tcaaccttcc ggcacgccgt aacgccatta acggttgtgc
    16081 aagcctgggg gcagcgatta atacaggggt aggtctgggg ctatacccgg attacgcaac
    16141 ggctgtcgat aaaatggttc gcgtgaaaga tatctttata ccgattgaga gcaatgccaa
    16201 acgctacgac gcgatgaata aaggcatttt caaagaccta accaaacata ctgatgtgat
    16261 cctgaaaaaa tcgtatgaag tgatgcatgg ggaattgggg aattggggaa tgcggattcg
    16321 atccagagct ggtcgaatgc gtgaataagg taggccgggc ggaaaactgc ccggcctgaa
    16381 catacctgaa tggttatccc cgctggcgcg gggaacataa gttcgacaac ttaaaaaatg
    16441 ataacaagtc cggtttatcc ccgctgatgc agggaacata ataaaaactt taactagttc
    16501 ggtttatccc tgtttttaca aggaa
//
LOCUS       DADMKF010000085        16499 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.086, whole genome shotgun sequence.
ACCESSION   DADMKF010000085 DADMKF010000000
VERSION     DADMKF010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16499
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.086"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            385..885
                     /gene="tssB"
                     /locus_tag="JNC24_002544"
     CDS             385..885
                     /gene="tssB"
                     /locus_tag="JNC24_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HAY9159263.1"
                     /translation="MSKKFEGSVAPRERINISYVPKTDGQTAEVELPLNMLVVGDTGN
                     TQETSPLDERQAVSVNKHNFGAVMAEAAIGLNFTVPATLKGSTTDDELNVALNIKSLD
                     DFSPDSVARQVPEVNKLLELREALTALKGPMGNLPAFRTQLQALLENEESREQLLKEI
                     GLVSNK"
     gene            920..1144
                     /locus_tag="JNC24_002545"
     CDS             920..1144
                     /locus_tag="JNC24_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9159264.1"
                     /translation="MTAHKNISGTYHLSVADILQVVYQVCFSPSVEINQDGVAALITT
                     LDRRISDLLDEIIHFYEFQQSASHWQRVLH"
     gene            1195..2670
                     /gene="tssC"
                     /locus_tag="JNC24_002546"
                     /pseudo
     CDS             1195..2670
                     /gene="tssC"
                     /locus_tag="JNC24_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011094952.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
     gene            2677..3090
                     /gene="tssE"
                     /locus_tag="JNC24_002547"
     CDS             2677..3090
                     /gene="tssE"
                     /locus_tag="JNC24_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HAY9159265.1"
                     /translation="MIPDRERTTGSLFERMEASSARNRQGGSIHSLRQSIRQNLRNIL
                     NTRSGSCRGAPELGIDEPEGAENFRESMSRAIEQCIERYEPRISHAEVQAVVSSASSP
                     LDMTFHITAWVTFNETHEVLEFDMAPNGSQHYRVD"
     gene            3094..4944
                     /gene="tssF"
                     /locus_tag="JNC24_002548"
     CDS             3094..4944
                     /gene="tssF"
                     /locus_tag="JNC24_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HAY9159266.1"
                     /translation="MEFEERYFREELDYLRQLSKLLATEKPHLARFLAEKDADPDIER
                     LLEGVAFLTGNLRQKIEDEFPELTHGLIKMLWPNYLRPVPAMTLIEYTPDMDKSSVPV
                     LIPRNEQFTTNAGEIRVDEVLPSDAKKEEPPPCTFTLCRDIWLLPVRLEQIENRSTTR
                     NGVINITFSVAPGTDFRTLDLNKLRFWLGNDDNYTRDQLYLWFCEYLQGADLTVGEQH
                     IRLPEFMLKAVGFEPQDAMLPWPKNVHSGYRILQEYFCYPDAFLFFDLCGCPALPDGL
                     QAEFFTLQLRFSRPLPVDIRLRRDSLRLYCAPAINLFIHHAEAITLDNRRADYPLVPS
                     RHYPQHYDVFSVNSVVSQVQDMFRKKDLGRPVSTQAARQWPAFESFSHQIEYSRKREV
                     VYWHHRTKTSLFHRGFDHTLAFIHADGSYPSDESLLSNEVVSVSLTCTNRELPSQIRS
                     GDITGTTGKNAAVASFRNITRPTQPLWPVIDGSLHWPLLSAMNLNYLSLLDTDALKQV
                     IANFDRHAIHHPQTARLSQQKLDAIERLETRPVDRLFTGIPVRGLASTLYLHPEPFVC
                     EGEMYLLGTVLSHFLSLYASVNSFHMLTVVNTESQETWKWTERIGQHPLI"
     gene            4908..5990
                     /gene="tssG"
                     /locus_tag="JNC24_002549"
     CDS             4908..5990
                     /gene="tssG"
                     /locus_tag="JNC24_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HAY9159267.1"
                     /translation="MDGKNRAASSYLSPGNPPADKEQNDPLAQVFHNACSYNFFAMAE
                     LLHRLAKGEKGTPELSLRDDPAQETLRFSADASLAFPCSDISALKRDTSGAFRMTTTF
                     MGLQGSQSPLPGYYLAHLAWKAVHEQSPVGDFLDMFSHRLTQFVWHIWRKYRYHISFR
                     NGGVDAFSQRMYSLVGLGHRQLRDKLAINHSKMLAYSGILANPGRSPEIICGLVSHCF
                     DLSEVTLQNWQRRKVDIEPDQQNSLGSYSLKNGEKLAGRSVLGNFVLGTRVPDLSGKF
                     QLSITSLTRKQFLSFLPSGENFLPLTMFVSFILRDQLAWDLHLGLAPEQVGAMRLGDN
                     KSALLGWTSFLGTPEERPSVTIRVRS"
     gene            6015..7295
                     /gene="tagH"
                     /locus_tag="JNC24_002550"
     CDS             6015..7295
                     /gene="tagH"
                     /locus_tag="JNC24_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated FHA domain
                     protein TagH"
                     /protein_id="HAY9159268.1"
                     /translation="MPEEKLQTLSLQVINGSELESGRAARCLFTQQGNVGHGPECHWS
                     VQDRQQSIPAQAFTVILHDGTFCLRPQTAQLWLNQAKVTATSDLIQLRQGDEIQIGRL
                     MVRVHLNRGDIPHYDEEMATPETIVTNRDMLTDTLLSTEGAPHYPGMTHRHQLADTVV
                     NGFSADPLQALQSESLITTGDPLSGIAAVRPSAPLSDPASNGGINTPFMDLPPIYASP
                     GDRNDDVSAAEMAQRHVAVTPLLRGLGGSLTMSNSDDADDFLEEAGRTLQAAIKGLLD
                     LQQQRNSLSDKHLRPLEDNPLRLNMDYATALDVMFAEGKSPVHLAAPAAVSESLRNVR
                     HHEEANRAAIVESLRVLLDAFSPQNLLRRFVQYRRSHELRQPLDDAGAWQMYSHYYEE
                     LASDRQQGFEMLFNEVYAQVYDRVLREKQREPEA"
     gene            7292..7816
                     /gene="tssJ"
                     /locus_tag="JNC24_002551"
     CDS             7292..7816
                     /gene="tssJ"
                     /locus_tag="JNC24_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HAY9159269.1"
                     /translation="MNRKAFLACVLMCVILTGCETAKKISEVIKNPDIQVGSLKEQPS
                     EITVTLLTEPDTNTNAEGESAAVDVQLVYLTDDSKLLAADYDQIASTPLPDVLGKNYI
                     DHQDFNLLPDTIKTLPPVKLDEKTQFIGVVAYFSDDQATEWKQIETVEGIGHHYRLLV
                     HVRQSSIEMKKEDE"
     gene            7819..9148
                     /gene="tssK"
                     /locus_tag="JNC24_002552"
                     /pseudo
     CDS             7819..9148
                     /gene="tssK"
                     /locus_tag="JNC24_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308252.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
     gene            9153..9914
                     /locus_tag="JNC24_002553"
     CDS             9153..9914
                     /locus_tag="JNC24_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotU family type IV/VI secretion system protein"
                     /protein_id="HAY9159270.1"
                     /translation="MDEGSLSLPPFTGHDEKSQRNYHLALRGNSLNPMIDAATPLLGM
                     VMRLSTMNSQTMPEHLFAQVVTDVQAVEQLLQEQGYEPGVIISFRYILCTFIDEAALG
                     NGWSNKNEWIKQSLLVHFHNEAWGGEKVFILLERLIREPKRYQDLLEFLWICFSLGFR
                     GRYKVAAQDQGEFEQIYRRLYHVLHKLRGDAPFPLLHQDKKTQGGRYQLISRLTVKHI
                     FCGGVVVLALFYLFYLLRLDSQTQDILHQLNKLLR"
     gene            9923..12700
                     /gene="tssH"
                     /locus_tag="JNC24_002554"
                     /pseudo
     CDS             9923..12700
                     /gene="tssH"
                     /locus_tag="JNC24_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308250.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
     gene            12697..13440
                     /gene="tagO"
                     /locus_tag="JNC24_002555"
     CDS             12697..13440
                     /gene="tagO"
                     /locus_tag="JNC24_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     TagO"
                     /protein_id="HAY9159271.1"
                     /translation="MSRLRVFRQTGWFIAGLMTGLPATAAPAEASSMAGVAVAVATTT
                     PPDATATLQAMQSCRRESAALERLDCYDRLLAPLSPSGFDGALVKAGFVGEAWTRATE
                     QEKRREGNTTELLVTQVPGERPTVVITTPAIGHVPPRPVLMFSCVDNITRMQVALMHP
                     LDVHDIAVTLNADNRALRSHWFVRENGTLLESSRGLSGIDEIKQLFGAKTLTVDTGAD
                     NAAGKLTFNIDGLARAIAPLRDACHWAGE"
     gene            13445..14857
                     /gene="tssA"
                     /locus_tag="JNC24_002556"
     CDS             13445..14857
                     /gene="tssA"
                     /locus_tag="JNC24_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HAY9159272.1"
                     /translation="MAMDLRDPNVWISHLLENLPEEKLASALKDDNPNWEYIDGEIVK
                     LGSLAHSQLDIPELQRRGLVILASESKDFRLLAHLLRTLQHAGDPHLALRLLALYVEH
                     YWAVAAPQNMAHKKRFASQVIKRFETGIEVFSQNAATAQRDALSGELAKLAQCWQSHN
                     APELAQATDDLFALYQRAFNRAAPAPVPTPAASGSSPQTTATSESGVTQPSAPAPQIV
                     IDSHDDKAWRDTLLKVAAILCERQPDSPQGYRLRRHALWQNITSTPQAESDSRTPLAA
                     VSADMVADYHAQLGSADMALWQQVEKSVLLAPYWLDGHCLSAQTALRLGYKQVADAIH
                     DEVIRFLERLPQLTGLLFNDHTPFISEQTKQWLAASPDAKVAPVAQIGEESKAARACF
                     AEQGLEAALRYLDMLPEGDPRDQFHRQYLAAQLTEEAGLVQLAQQQYRMLFRMGLQMM
                     VADWEPSLLEQLEQKFTAEQ"
     gene            14876..>16499
                     /gene="tssM"
                     /locus_tag="JNC24_002557"
     CDS             14876..>16499
                     /gene="tssM"
                     /locus_tag="JNC24_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
                     /protein_id="HAY9159273.1"
                     /translation="MFRLPTPRLLSGLKSALRPAMPRFKVSAFWLLILAWIFLLVWIW
                     WKGPMWTLYEEQWLKPLANRWLATAAWGIIALVWLTVRVMKRLQQLEKMQKQQREEAV
                     DPLSVELNAQQRYLDRWLLRLQRHLDNRRFLWQLPWYMVIGPAGSGKTTLLREGFPSD
                     IIYAPEGARGAEQRLYLTPHVGKQAVIFDIDGTLCAPADADILHRRLWEHALGWLKEK
                     RARQPLNGIILTLDLPDLLTADKRRREHLLQTLRSRLQDIRQHLHCQLPVYVVLTRLD
                     LLQGFAALFQSLNRQDRDAILGVTFTRRAHENDDWRTELNAFWQTWVDRMNLALPDLM
                     VAQTHTRASLFSFSRQMQGSREPLVSLLEGLLDGENMNVMLRGVYLTSSLQRGQMDDI
                     FTQSAARQYRLGNNPLASWPLVDTAPYFTRSLFPQALLAEPNLATESRAWLIRSRRRL
                     TVFSATGGVAALLLITGWHHYYNGNYQSGITVLKQAKAFMDVPPPQGEDDFGNLQLPL
                     LNPVRDATLAYGDWGDRSRLADMGLYQGRRIGP"
BASE COUNT         3807 a         4557 c         4553 g         3582 t
ORIGIN      
        1 aatttttgag atattcctta aaaataccca cttaatcaat cacaaaacaa atatataaac
       61 aagcaatcat tagattagct tatttttaat gaaattatta tatacttcaa taagttacac
      121 aaaaactcta tatttaaatt attgccataa tacacctgaa gcgaattgca caggttcaca
      181 caatctattt aacgcttgca gaatacccct ctttacgatc gataaaaagt ccttttctta
      241 tttattttga agcgtaaaat gcggggtgat gaaggagtgg tgattttatt ttcaccgcca
      301 ttagggcggc attgccgata tttttttgcg gtacaaatat gccgtgttca tggcctgtac
      361 gagataacat gacggatcac ttttatgagc aaaaaatttg aaggcagcgt cgcgccccgc
      421 gagcgtatta atatatcgta tgtcccaaaa acagatggcc agacggctga agtggaactt
      481 ccgcttaaca tgctcgttgt cggcgatacc ggaaatacgc aggaaacatc cccactggat
      541 gaacgtcagg cagtttccgt taacaagcac aatttcggtg cagtgatggc tgaagcagcc
      601 attggcctca attttactgt ccccgccacg ctgaagggaa gcacaacaga cgatgaactg
      661 aatgtggcgc tgaatatcaa atcactggat gatttttcac cagatagcgt ggcccggcag
      721 gttccggaag tgaataaatt gctggaactg cgtgaagcac tgactgcact gaaggggccg
      781 atgggtaacc ttccggcatt ccgtacccag ctccaggcat tgctggaaaa tgaagagtcc
      841 cgcgagcagt tgctcaaaga aatcggtctg gtgagcaata aataaccacc actatttcat
      901 taaacagggt tatccaggca tgacggcaca caaaaatatc tccgggacat accacctctc
      961 tgttgcagac attctgcagg tggtttacca ggtatgtttc agcccgtccg ttgaaatcaa
     1021 ccaggatggt gtggctgcgc tgattacaac actggatcgc cgtatcagtg atctattgga
     1081 tgagattatc catttttatg agtttcagca gagcgcgtcg cactggcagc gcgtacttca
     1141 ctgatggcga aaagccggtt tcagaaacac agctaaaaca gaaggatttt cagtatgtct
     1201 ttacaggaag aggaactcgt ttccagtcat gccgggcagc ctgagcaggc atcgtcgctg
     1261 cttgatcaaa tcatggcaca gacccgtatt cagcccggtt cggaaggtta tgacgttgcc
     1321 ctccagggtg tgacagcctt tattgccagc atcctgcaat ccactgcatc tgccgaaccg
     1381 gtcaataaac tggccgtgga cagcatgatt gcagatattg atgaacgcat cagccgccag
     1441 atggatgtca tcatccatgc gcctgccttt cagcaggtcg agtcgttctg gcgctcactg
     1501 aaaaccatgg tggatcgcgt tgatttccgt gaaaacatca aggtcaacgt gcttcatgtc
     1561 accaagcagg aacttctgga agactttgag tttgcgccag aaattattca gtccggattt
     1621 tataagcacg tttattcgtc cggtttcgga cagtttggcg gtaaacccat cgctgccgtg
     1681 ctgggcgctt atgagttcaa aaataccgcg cctgacatga aactgctgca atacgtcagt
     1741 gccgtgggcg caatggcgca cgcaccgttc ctgtcctccg tttccccgga gtttatgggg
     1801 ctgaactcgt ggaccgaact gcctaatatc aaagatcttt atgccatctt tgaaggtccg
     1861 gcctacacca aatggcgcgc cctgcgtgac tcggaagatt cgcgctattt agggcttacg
     1921 gcaccccgct tcctgcttcg ccagccctat tcaccaacag ataaccctgt taagaacttt
     1981 aactattacg aagatgtcag ccagaaccac gaagattatc tgtggggtaa tacggcctgg
     2041 atgctggcat gcaatatcgc agacagtttt gccaaatacc gctgatgtcc aaacattatc
     2101 ggtccgcaaa gcggcggcgc agtgaaagat ctgccgatgc atctgttcga aacgatgggg
     2161 cagattcagg ccaaaatccc aaccgaggtg ctggtcaccg atcgccgcga atttgaactg
     2221 gctgaagagg gttttatcac ccttaccatg cgtaaagact ctgataatgc agcctttttc
     2281 tctgcaaact cggtacaaaa accgaagcac tttccgggaa aagatgcaga aaccaactat
     2341 aagctgggta cgcagcttcc ttatctcttc atcatcaatc ggttagcgca ctacatcaag
     2401 gtgttgcagc gtgaacaact ggggtcatgg aaagaaagga gcgacttaga aagagagctt
     2461 aatacctgga tccgtcagta tgttgccgac caggaaaacc cgcctgctga cgtgcgcagc
     2521 cgcaaacctc tgcgcgctgc caaagttgag gttatggatg tagaaggcga acccggctgg
     2581 taccaggttg cgctaagcgt gagacctcat tttaaattta tgggggcaaa ttttgagctt
     2641 tccctggttg gccggttaga cagggagtaa cacaccatga taccggatcg ggaaagaacc
     2701 acaggttctc tttttgagcg tatggaagcc tcttcggcca gaaaccggca gggaggcagt
     2761 attcattcgc tcaggcaatc cattcgccag aacctacgaa acattcttaa tacccggtcc
     2821 ggtagttgcc ggggtgcgcc tgagctgggc attgatgaac cggaaggggc tgaaaacttc
     2881 cgcgaatcca tgagtcgggc catcgagcaa tgtatagaac gctatgaacc ccggatctca
     2941 cacgctgaag tgcaggcagt ggtttcatcc gcctccagcc cgctggatat gacgtttcat
     3001 attacagcct gggtgacatt taacgaaacg catgaggtgc ttgaatttga tatggcaccg
     3061 aatggcagcc agcattaccg ggtggactga caaatggaat ttgaagaacg ctatttccgg
     3121 gaagaactcg attacctgcg ccagcttagc aagctgctgg caacggaaaa accccatctg
     3181 gcccgcttcc tggccgaaaa agatgcagat ccggatattg aacgcctgct ggaaggggtg
     3241 gcttttctta ccggcaatct ccgccagaaa attgaggatg aattcccaga actgacgcac
     3301 gggcttatta agatgctatg gcctaattac ctgcgtccgg ttccggcaat gacccttatt
     3361 gaatatacgc cggatatgga taagtcttct gtaccggtgt taatcccccg taatgagcag
     3421 tttacaacca acgccgggga aatcagagtt gatgaagtgc tgccctctga tgctaaaaag
     3481 gaggagccgc ctccctgtac cttcaccctc tgccgggata tctggctgct gcccgttcgc
     3541 ctggagcaga ttgaaaaccg cagtacgacc cgtaatggtg ttatcaacat caccttttcg
     3601 gtcgcaccgg gaacggactt ccgcacgctg gatctgaaca aacttcgctt ctggctcggc
     3661 aatgacgaca actatacccg tgaccagctt tatttatggt tctgcgaata cttgcagggt
     3721 gccgacctga ctgtgggtga acagcatatt cgcctgcctg agtttatgct aaaagctgtc
     3781 ggttttgagc cgcaggatgc catgctgccc tggccgaaaa acgtccacag cggctaccgg
     3841 atccttcagg agtatttctg ttaccccgat gcgtttctct tttttgatct ttgtggttgt
     3901 ccggctttgc ctgacggatt gcaggcggaa ttctttaccc tgcaactgcg tttttcgcgc
     3961 cctttgcccg tggacatccg gctgcgccgc gattccctgc gcctgtattg cgcacctgcc
     4021 attaatttat ttatccacca tgcagaagcc atcacgctgg acaaccggcg ggcagactat
     4081 ccgctggttc ccagccgcca ttacccacaa cattacgatg tattttccgt taacagtgtg
     4141 gtgagccagg tccaggatat gttcaggaaa aaagatctgg ggcgtcctgt ttcgacgcag
     4201 gccgcgcgcc agtggccagc ctttgaaagt ttcagccatc agatagaata cagccggaag
     4261 cgggaagtgg tgtactggca tcaccggacc aaaacatccc tgttccatcg cggctttgat
     4321 catacccttg cctttataca tgctgatggc agttatccgt cagacgaatc tctgctcagt
     4381 aatgaagtgg tttcggtatc gctgacctgt accaaccgtg agcttccgtc acaaattcgt
     4441 tccggcgata tcaccggcac aaccggaaaa aatgcggctg ttgcttcatt tcgcaacatt
     4501 acccgcccga cgcaaccact ctggccggtc attgatggca gcctgcactg gcccctactc
     4561 tccgccatga acctgaatta tctgtcatta ctggatacgg acgcgctgaa gcaggtcatc
     4621 gccaactttg atcgccacgc aatccatcat ccgcagacgg cacggctgtc acaacaaaag
     4681 ctggatgcca ttgagcgtct ggagacccgc cccgttgatc gcctgtttac gggtattccc
     4741 gtccgggggc tggcttccac gctgtatctg cacccggagc cgtttgtctg tgaaggggaa
     4801 atgtatctgc tcggtacggt gctttcgcat tttctgtcgc tgtacgccag cgttaactca
     4861 ttccacatgc tgaccgttgt gaacacagaa agccaggaga catggaaatg gacggaaaga
     4921 atcgggcagc atcctcttat ctgagtccgg gaaatccccc ggcagataaa gagcagaatg
     4981 atccgctggc acaggttttc cataatgcct gctcttacaa tttttttgcg atggcggagc
     5041 tgttgcaccg cctggcaaag ggtgaaaagg ggacgccaga attatccctg cgcgacgatc
     5101 cggcacagga aaccctgcgt ttcagcgccg atgccagcct tgccttcccg tgcagtgata
     5161 tcagtgcgct gaaacgggat acgtcagggg cattcaggat gaccaccacc tttatggggt
     5221 tacaggggag ccagtcgcct ctccccggtt attacctcgc tcacctggcc tggaaagcgg
     5281 tacatgaaca aagtccggta ggtgattttc tggatatgtt cagccaccgc ctgacccagt
     5341 ttgtctggca tatctggcgt aagtaccgct atcacatcag ttttcgtaac ggcggtgtgg
     5401 atgcgttctc gcagcgcatg tactcccttg tcgggctggg ccaccgccag cttcgcgata
     5461 agctggcgat taatcacagc aaaatgctgg cgtattccgg gatcctggca aatccgggac
     5521 gatccccgga aattatctgt ggcctggtgt cccactgctt tgatttgtca gaagtgacac
     5581 tgcaaaactg gcaacgtcgc aaggtggata ttgaaccgga tcagcaaaat agtctgggga
     5641 gctattctct taaaaatggc gaaaaactgg cgggaagatc ggtactgggc aactttgttc
     5701 tgggtacacg agtgcccgat ctcagcggaa aattccagct cagtattacc agcctgaccc
     5761 gaaaacagtt cctctctttt ctgccgtccg gcgaaaactt cctgccactg acgatgttcg
     5821 tgtccttcat cctgcgcgat cagcttgcct gggacttaca tctggggctg gcgccggagc
     5881 aggtgggcgc aatgcgcctg ggcgataaca aaagtgcgct gctgggctgg accagcttcc
     5941 tcggcacacc ggaagaacga ccctcagtca ccatcagggt acggtcgtaa attatgataa
     6001 tcacggagca gaatatgccg gaagagaaac tacagacgct ttcattgcag gtcattaacg
     6061 gcagtgagct ggaaagcggg cgggcggcgc gctgtctgtt cacacagcag ggaaatgtcg
     6121 gccatggccc cgaatgccac tggtcggtac aggatcgtca gcagagcatt ccggcccagg
     6181 cttttaccgt tatcctgcac gatggcacat tttgtctgcg cccgcagacg gcacaactgt
     6241 ggctgaatca ggcaaaagtc acagcaacat cagacctaat acagttgcgc cagggcgatg
     6301 agatccagat cggacggctg atggtgaggg ttcatctgaa ccggggagat attccccatt
     6361 acgatgagga aatggccact cccgaaacca tcgttaccaa tcgcgatatg ctcacggata
     6421 ccctgctatc aacggagggt gcgccacact atccgggaat gactcaccgg caccagcttg
     6481 cagacaccgt ggtaaacggt ttttctgccg atccactcca ggctcttcag tccgaaagcc
     6541 tgattaccac gggcgatccg ctttcaggca ttgcggctgt ccggccatcg gcaccgctgt
     6601 ccgatccggc aagtaatggg gggatcaata ctccgtttat ggatctgccg cccatttatg
     6661 ccagccccgg cgatcgtaat gacgacgtct ctgcggcaga aatggcacaa cgccacgttg
     6721 cggtcacccc cttactgcgc ggtcttggcg gctcgcttac catgagcaat tccgacgatg
     6781 cggatgattt tctggaggag gccggacgaa cgttacaggc cgcaataaaa ggtctgctcg
     6841 atttgcagca gcagcgtaac agcctctcag acaaacattt gcgcccgctg gaagataacc
     6901 cgctgcgcct gaacatggat tacgccaccg cgctcgacgt aatgtttgcc gaaggtaaaa
     6961 gcccggtaca tctggcggct cccgccgccg tcagtgaaag cctgcgcaat gtccgccacc
     7021 acgaagaagc taaccgggca gcgattgtgg agtcgcttcg tgtcctgctg gatgctttct
     7081 caccacaaaa tctgctgcgc cgctttgtgc agtaccgccg cagccatgaa ctgcgccagc
     7141 cgctggatga tgccggagca tggcaaatgt acagccatta ttacgaagaa ctggcctccg
     7201 atcgccagca ggggtttgag atgctgttta acgaggtcta cgcccaggtc tatgaccggg
     7261 tgcttcgtga aaaacagcgg gagccggaag catgaacagg aaggcgtttc tggcctgcgt
     7321 gctgatgtgc gtcatattaa ccggctgcga aacggcgaaa aaaatcagtg aggtgatcaa
     7381 aaacccggat atccaggtcg ggagcctgaa ggagcaacct tcggaaataa ccgtcaccct
     7441 gctgaccgaa ccggacacca ataccaatgc ggaaggtgaa tcagccgccg tggatgtgca
     7501 actggtgtac ctgactgacg actcaaaatt gctggcagcc gactatgacc agatagccag
     7561 cacgccgcta ccggatgtgc tgggtaaaaa ctatatcgac caccaggatt tcaacctgtt
     7621 gccggacacc atcaaaaccc tgccgccggt aaagctggat gagaaaacgc agtttattgg
     7681 tgtcgtggcc tatttctccg atgaccaggc cactgaatgg aaacaaatcg agacggtgga
     7741 aggaatcggc caccattacc gcctgctggt gcatgtacgt caaagcagca tcgaaatgaa
     7801 aaaagaggat gaataattat ggcaaccacc cgcaacaagg tgatgtggca ggaagggatg
     7861 ctgatgcgtc cacaccattt ccagcagcag cagcgttaca acgactacct ggataaccag
     7921 cgtttccggg ccatgaatga tttatcctgg ggatttaccg aactcaccct caacaatgaa
     7981 ctgctggcgc agggtaagat catgattgac agcgcgtcag gcacactgcc cgacggcacc
     8041 gtcttttcta tccccgacca ggacgcactg cccgatccgc tgcacccgca aaattttccg
     8101 gacgaaagaa gccgcaatat ctacctcgct ctgccggtcg ccagtgatgt gagaaatgaa
     8161 atcagcgacg gacggcgaat cgggcgttac cggctgaatt atgccgatgt ccgggatttg
     8221 cattcagaag aaggtgacgc gcgaacattg acgctgggtc aactgacgcc gcgcattatg
     8281 agcggtgcag aagatatgag cgcctatatt acgctcccgc tttgccgtat cagcgatcgc
     8341 cgccgatggc tccctgacgt tggatgacga ttttatcccc tcctgccaga atattcaggt
     8401 cagtaagaaa ctgcgtgttt atctcaaaga ggtacagggg gccattggcg gacgggcaag
     8461 cgatctggca aaccgtattg gctctccggc gcagagcggc atcgcggatg tggcggaatt
     8521 tatgatgttg cagttactta accgtaacca gacccggttt acccatcacg cccgtcgatc
     8581 ccagctccac ccggaagatt tctaccttga tcttgccggg ttgctgggtg aactgatgac
     8641 ctttaccgag ccgtcgcgcc tgccctgccc gcttgatgtg tatgatcatc atgacctgac
     8701 caaaatattt aaaacgctgt taccggaagt caaacgggcg ctgcataccg tactgtcgcc
     8761 aagagccgtc aatctgccgc tgcatctgcg tgacggtatc tggcaggccg atatccatga
     8821 cacagaactg ctgcaatctg ccacctttgt gctggccgtg gcggcaaaca tgccggtcga
     8881 tcagatccag cgccagttta tccagcagtc gaaaatttcc tcgccggaaa aaatccgcaa
     8941 tatggtcagt gtgcagatcc ccggtattcc attgcgtgcc cttatggtgg ctccccgcca
     9001 gcttccttac cattccgggt tcagctattt cgaactcgac aagagtggcc aggcctggac
     9061 agaaatggct gccgccgggg ccgttgcact gcatgtttcc ggcagtttcc cggatctgaa
     9121 catgcaactg tgggcgataa gagggtaaga cgatggatga ggggtcactg tctctgccac
     9181 catttaccgg acacgacgaa aaaagccagc gcaactacca tctcgcgctt cgtggcaaca
     9241 gcctgaatcc gatgatcgac gcggccacgc cgctactcgg tatggtaatg cgcctttcaa
     9301 ccatgaacag ccagacgatg ccggagcatc tttttgctca ggtggtgaca gatgttcagg
     9361 ccgtggagca actgcttcag gagcagggct acgaaccggg cgtgattatc tcgtttcgct
     9421 acatcctctg cacgtttatt gatgaagcgg cgctcggtaa cggctggtca aacaagaacg
     9481 agtggatcaa gcagtcattg ctggtccatt tccacaacga agcctggggt ggcgagaaag
     9541 ttttcatcct gctggagcgc ctgatccgtg aacccaaacg ttaccaggat ctgctggaat
     9601 ttctctggat ctgtttttct ctgggatttc gcgggcgcta caaagtggca gcacaggatc
     9661 agggggaatt tgagcagatc taccgccgtc tgtaccacgt tctgcataaa ctgcgcggcg
     9721 atgccccttt cccgctgtta caccaggaca aaaaaaccca gggcgggcgt tatcagctca
     9781 tcagtcgcct gactgttaaa catatttttt gtggtggcgt tgtcgtgctg gcgttgtttt
     9841 acctgttcta tctgttgcgg ctggacagcc agacgcagga catcctgcac cagctaaaca
     9901 aattacttcg ataaggacgt ttatgatcca gattgatctt cccacgctgg taaaacggct
     9961 gaacctgttc tcccgccagg cgctggagat ggccgcctct gaatgtatga gtcagcaggc
    10021 agcggaaatt accgtcagcc atgtgctcat tcagatgctc gccatgccac gcagtgacct
    10081 gcgggttatt acccgccagg gcgatattgg catggaagag ttgcgccagg cgctgacggt
    10141 agagaactac acaaccgccc gttctgcgga cagctacccg gcgttttccc cgatgctggt
    10201 tgagtggctt aaagagggct agctgctggc gtcggctgag atgcagcaca gcgaactgcg
    10261 cggcggcgtg ttgctgctgg ccctgctgca ttcgccgctg cgttatatac cgcctgctgc
    10321 cgcccggctg ttgaccggca ttaaccgtga ccgtctgcaa caggactttg tgcagtggac
    10381 acaggagtcg gcggaatcag tcgtgccgga tgcagacggt aaaggcgcag gcacaatgac
    10441 ggacgcctct gacaccctgc ttgcccgcta tgccaaaaac atgaccgcag acgcccgtaa
    10501 cggcaggctt gacccggtac tgtgccgcga ccacgaaatc gacctgatga tcgacattct
    10561 ctgccgccgc cgtaaaaaca acccggtggt ggtgggcgaa gcgggcgtgg gcaaaagcgc
    10621 actgattgaa gggctggcgc tgcgcatcgt ggcaggccag gtgccggaca agctgaaaaa
    10681 caccgatatc atgacccttg acttgggcgc attgcaggcc ggggcgtcgg tgaagggtga
    10741 attcgaaaaa cgtttcaaag ggctgatggc ggaggtcatt tcctccccgg tgccggtcat
    10801 tctgtttatc gacgaagcac ataccctgat tggcgcgggc aaccagcagg gcgggctgga
    10861 tatctccaac ctgctcaaac cggcgctggc gcgcggcgag ctgaaaacca tcgccgccac
    10921 cacctggagc gagtacaaaa aatacttcga aaaagatgcc gccctgtcgc gccgcttcca
    10981 gttggtgaag gtcagcgaac ccaacgctgc cgaagccacc attattctgc gcggtctgtc
    11041 ggcggtctat gaacagtctc acggcgtgct gattgatgat gacgccttgc aggccgctgc
    11101 gacattaagc gagcgttatc tctccgggcg tcagttaccg gacaaagcga ttgatgtgct
    11161 ggataccgcc tgcgcccgtg tggccatcaa cctgtcgtcg ccgccgaagc aaatctcggc
    11221 gctgaccact ctgagccacc agcaggaggc ggaaattcgc cagcttgagc gcgagcttcg
    11281 catcggactg cgtaccgaca catcacggat gaccgaggtg ctggtgcagt atgatgaaac
    11341 gctgacggcg ctggatgaac tggaagcggc ctggcaccag cagcagacgc tggtccggga
    11401 gattatcgcg ctgcgccagc agttactggg cgtggcagag gacgatgcgg cgccgttgcc
    11461 ggacgcagat accgtggagg atacgcagcc agagtcagag tcagagtcag agtcagagca
    11521 ggataatacc ggtgccgaac cggctgatga ggccgacaga gaacaaccgg aagagaccgc
    11581 tgaaacagtt tccccggtac agcggctggc ccagctcact gccgaactgg acgccctgca
    11641 taacgaccgg ttgctggtct ccccgcacgt cgataaaaaa cagattgcgg cggtgattgc
    11701 cgaatggacc ggcgtgccgc tcaaccgcct gtcgcagaat gaaatgtcgg tcatcaccga
    11761 cctgacgaaa tggctgggtg acaccatcaa aggccaggac ctggcgattg ccagcctgca
    11821 taagcatcta ctgaccgcac gcgccgacct gcgtcgtccg ggacgcccgc tcggtgcgtt
    11881 cctgctggct ggccccagcg gtgtgggtaa aaccgaaacc gtcctgcaac tggcagagct
    11941 gctctacggc ggtcgccagt acctgaccac catcaatatg tccgaattcc aggagaaaca
    12001 caccgtctcg cggctgattg gttcgcctcc gggctacgtt ggctacggtg aaggcggcgt
    12061 actgaccgaa gcgattcgcc agaaacccta ctcggtagta ctgctcgatg aagtggaaaa
    12121 agcgcacccg gatgtgctca acctgttcta ccaggcgttc gataagggcg aaatggcaga
    12181 cggtgaaggc cgcctgattg actgcaaaaa tatcgtcttc ttcctgacgt ccaacctcgg
    12241 ctaccaggta atagtcgagc atgccgatga cccggaaacc atgcaggaag tacagtatcc
    12301 ggtgctggcc gacttcttca aacctgccct gctggcgcgt atggaagtgg tgccgtatct
    12361 gccgctgtcg aaagagacgc tcgccaccat tatcgccgga aaactggccc gtctggataa
    12421 cgtgctgcgc agtcgctttg gtgtagaagt ggtcattgaa ccggaagtga cggacgaaat
    12481 catgagccgc gtcacccgcg cggaaaacgg cgcgaggatg ctggaatcgg tcatcgatgg
    12541 cgacatgcta ccgccgctct cgctgctgct gttgcagaaa atggcggcta acacggcgat
    12601 tgcccggatt cggttgtcgg cagtggacgg cgcatttacg gcagacgtgg aagatgctca
    12661 gaacgacgag tccgtcacaa aggatgaaac ggttttatga gcaggttgcg cgttttccgg
    12721 cagacaggat ggtttatcgc gggcctgatg acgggcttac ccgccactgc cgcgcccgca
    12781 gaagcatcgt caatggccgg tgtggcggtc gccgtcgcca ccacgacccc gccggatgcg
    12841 acagccacgt tacaggccat gcagtcctgt cggcgggagt ccgcggcgct ggagcggctt
    12901 gactgctatg accgcctgct ggcaccgctg tccccgtcag ggtttgacgg ggcgctggtc
    12961 aaagccggtt ttgtgggcga ggcctggacc cgcgccacag agcaggaaaa acgccgcgag
    13021 ggcaacacca cagaactgct ggtgacgcag gtgccggggg agcgcccgac ggtggtgata
    13081 accaccccgg ctatcggtca tgttccgcca cgcccggtgc tgatgttcag ttgcgtggac
    13141 aatatcaccc ggatgcaggt ggcgctgatg caccctctgg atgtccatga tatcgccgtc
    13201 actctgaatg ccgacaaccg ggcattgcgc agccactggt ttgtgcgcga gaacggcacc
    13261 ctgctggagt ccagccgggg gttatccggg attgatgaaa ttaagcaact gttcggcgca
    13321 aaaacgctga cggtggatac cggcgcagac aatgccgccg gaaagctgac ctttaacatt
    13381 gacgggctgg caagggcgat tgctcccctg cgcgatgcct gccactgggc gggagaataa
    13441 aaatatggca atggacttac gcgatccgaa tgtctggata tcgcacctgc tggaaaacct
    13501 gccggaagag aaactggcat cggcgctgaa agatgacaac ccgaactggg agtatatcga
    13561 cggcgaaatc gtcaagctgg gatcgctggc ccacagtcaa ctggatatcc cggaactcca
    13621 gcgccggggg ctggtcattc tggcttcgga gagtaaggat tttcgtctgc tggcgcatct
    13681 gctaagaacc ctgcaacacg ccggtgatcc tcatctcgca ttgcgcctgc tggcgctgta
    13741 tgtggagcat tactgggcgg tggctgcgcc gcagaatatg gcgcataaaa aacgctttgc
    13801 cagccaggtc attaaacgct ttgaaacagg tattgaagtt ttttcacaga acgccgccac
    13861 agcgcagcgc gatgcgctgt caggggaact ggcaaaactg gcgcaatgct ggcagtcaca
    13921 taacgccccg gaactggcac aggctactga tgatcttttt gccctgtacc agcgtgcgtt
    13981 taatcgtgcg gcaccggctc ctgtccccac tccggcggcc tccggtagtt caccacagac
    14041 caccgccacg tctgaaagcg gcgtgacgca acccagtgct ccggctcccc aaatcgtcat
    14101 cgacagtcac gacgacaaag cctggcgcga cacgctgtta aaagtggcgg ccattttatg
    14161 tgaacgccag ccggattcac cgcagggcta ccgtctgcgc cgccatgcgc tttggcagaa
    14221 catcaccagt acgccccagg cggaaagcga tagccgtact ccactggctg cggtctccgc
    14281 cgatatggtg gcagattacc atgctcagct cggtagtgcc gatatggcgc tgtggcagca
    14341 ggtggagaaa agcgtactgc tggctcctta ctggctggat ggtcactgcc tttctgcaca
    14401 gacggcgctg cgtctgggtt acaaacaggt ggcagacgcc atccacgatg aggtcatccg
    14461 cttccttgag cgtctgcccc agcttaccgg gctgctgttt aatgaccaca caccgtttat
    14521 cagtgagcag acgaaacaat ggctggcagc ttcgcccgac gccaaagttg caccggttgc
    14581 gcaaatcggt gaggaatcga aggcagccag agcctgtttt gctgagcagg gtctggaggc
    14641 ggcgctgcga tatctggaca tgctacccga aggcgatccc cgcgatcagt ttcaccgcca
    14701 gtaccttgcc gcacagttga cggaagaggc ggggctggta cagcttgcgc agcagcagta
    14761 ccggatgttg ttcaggatgg gattgcagat gatggtggca gactgggagc cgtccctgct
    14821 tgagcagcta gaacaaaaat tcacggcaga acaataaaag agaacaggag tttttgtgtt
    14881 cagattacca acaccccgac tactcagcgg actcaaatca gccctgcgac cggcgatgcc
    14941 ccggtttaaa gtctctgctt tctggctgct gatactggcg tggatttttc tgctggtgtg
    15001 gatctggtgg aaaggcccga tgtggacgct gtatgaagaa cagtggctca aaccactggc
    15061 gaaccgctgg ctggcaacgg cggcgtgggg gattattgcc ctggtgtggc ttaccgtccg
    15121 ggtgatgaag cgcctgcaac agcttgaaaa aatgcaaaag caacagcgcg aggaagccgt
    15181 tgatccgctc agcgtggaac tgaacgccca gcagcgttat cttgaccgct ggctgctgcg
    15241 cctgcaacgc catctcgaca accgccgttt cctgtggcag ttgccgtggt atatggtcat
    15301 cggcccggcg ggcagtggca aaacgacact gctgcgcgag gggtttccgt ccgacattat
    15361 ttatgccccg gagggcgcac gcggcgcaga acaacgcctg tacctcacgc cccatgtcgg
    15421 taaacaggcg gtgatctttg atatcgacgg cacactctgc gctcccgctg atgcggatat
    15481 cctgcatcgc cgtctgtggg aacatgccct cggctggctg aaagaaaagc gcgcgcgcca
    15541 gccgctgaac gggattattc tgacactcga tttacccgat ctgctgaccg cagacaaacg
    15601 ccgccgcgag catctgttac agacgctgcg cagccgctta caggatatac gccagcatct
    15661 tcactgccag ttaccggttt acgtggtact gacccggctt gatttattac agggttttgc
    15721 cgccctgttc cagtcactca acagacagga tcgcgatgcg attctgggcg tcacgttcac
    15781 ccgccgtgcc catgaaaatg atgactggcg aacagagttg aatgctttct ggcagacatg
    15841 ggtggatcga atgaatctgg cgttgccgga tctgatggtc gctcagactc acacccgcgc
    15901 gtctttattc agtttttccc gccagatgca gggaagccgt gaaccgctgg tgtcactgct
    15961 tgagggtctg cttgatggcg aaaatatgaa cgtgatgctg cgtggtgtct atctcacctc
    16021 ttcgcttcag cgtggacaga tggatgatat attcacccag tctgccgccc gccagtaccg
    16081 gctgggcaat aacccactgg cgtcctggcc cctggtggac accgcgcctt atttcacccg
    16141 cagcctgttc ccgcaggcat tactcgcaga gcctaatctg gcaacagaga gccgcgcctg
    16201 gctgatacgt tcccgtcgcc gcctgacggt tttctctgcc acaggcggcg tggcagcact
    16261 gctgctcatc accggctggc atcactatta caacggtaac tatcagtccg gcatcaccgt
    16321 gcttaagcag gccaaagcct ttatggacgt gccgcctccg cagggggaag atgactttgg
    16381 caacctgcaa ctgccgctcc tgaacccggt acgcgatgcc acactggcct atggcgactg
    16441 gggcgaccgc agccgtctgg ccgatatggg actgtaccag ggacgacgta tcgggcctt
//
LOCUS       DADMKF010000086        16425 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.087, whole genome shotgun sequence.
ACCESSION   DADMKF010000086 DADMKF010000000
VERSION     DADMKF010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16425
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.087"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            66..1712
                     /gene="mqo"
                     /locus_tag="JNC24_002558"
     CDS             66..1712
                     /gene="mqo"
                     /locus_tag="JNC24_002558"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HAY9159274.1"
                     /translation="MKKVTAMLFSMAVGLNAVSMAAKAKASEEQETDVLLIGGGIMSA
                     TLGTYLRELEPEWSMTMVERLEGVAQESSNGWNNAGTGHSALMELNYTPQNADGSISI
                     EKAVAINEAFQISRQFWAHQVERGVLRTPRSFINTVPHMSFVWGEDNVNFLRARYAAL
                     QQSSLFRGMRYSEDHAQIKEWAPLVMEGRDPQQKVAATRTEIGTDVNYGEITRQLIAS
                     LQKKSNFSLQLSSEVRALKRNDDNTWTVTVADLKNGTAQNIRAKFVFIGAGGAALKLL
                     QESGIPEAKDYAGFPVGGQFLVSENPDVVNHHLAKVYGKASVGAPPMSVPHIDTRVLD
                     GKRVVLFGPFATFSTKFLKNGSLWDLMSSTTTSNVMPMMHVGLDNFDLVKYLVSQVML
                     SEEDRFEALKEYYPQAKKEDWRLWQAGQRVQIIKRDADKGGVLRLGTEVVSDQQGTIA
                     ALLGASPGASTAAPIMLDLLEKVFGDRVSSPQWQATLKAIVPSYGRKLNGDVAATERE
                     LQYTSEVLGLKYDKPQAADSTPKPQLKPQPVQKEVADIAL"
     gene            complement(2021..2509)
                     /gene="eco"
                     /locus_tag="JNC24_002559"
     CDS             complement(2021..2509)
                     /gene="eco"
                     /locus_tag="JNC24_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HAY9159275.1"
                     /translation="MKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAEKGMKRQV
                     IQLTPQEDESTLKVELLTGQTLEVDCNLHRLGGKLENKTLEGWGYDYYVFDKVSSPVS
                     TMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAEEKIDNAV
                     VR"
     gene            complement(2644..2808)
                     /locus_tag="JNC24_002560"
     CDS             complement(2644..2808)
                     /locus_tag="JNC24_002560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9159276.1"
                     /translation="MHTPIGVKPVAGSKEWREAWQKRAFAHISNGYKHIYIAINSPEI
                     FLLVCSLIRI"
     gene            2917..3411
                     /gene="napF"
                     /locus_tag="JNC24_002561"
     CDS             2917..3411
                     /gene="napF"
                     /locus_tag="JNC24_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HAY9159277.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGAGGYPSVNFKNNECCFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQPQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            3401..3664
                     /gene="napD"
                     /locus_tag="JNC24_002562"
     CDS             3401..3664
                     /gene="napD"
                     /locus_tag="JNC24_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HAY9159278.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            3661..6147
                     /gene="napA"
                     /locus_tag="JNC24_002563"
     CDS             3661..6147
                     /gene="napA"
                     /locus_tag="JNC24_002563"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HAY9159279.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNRRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            6154..6849
                     /gene="napG"
                     /locus_tag="JNC24_002564"
     CDS             6154..6849
                     /gene="napG"
                     /locus_tag="JNC24_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HAY9159280.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            6836..7699
                     /gene="napH"
                     /locus_tag="JNC24_002565"
     CDS             6836..7699
                     /gene="napH"
                     /locus_tag="JNC24_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HAY9159281.1"
                     /translation="MANRKRDAGREALVKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSCSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILLGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AASDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            7696..8145
                     /gene="napB"
                     /locus_tag="JNC24_002566"
     CDS             7696..8145
                     /gene="napB"
                     /locus_tag="JNC24_002566"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HAY9159282.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            8155..8757
                     /gene="napC"
                     /locus_tag="JNC24_002567"
     CDS             8155..8757
                     /gene="napC"
                     /locus_tag="JNC24_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HAY9159283.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSRLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            8776..9393
                     /gene="ccmA"
                     /locus_tag="JNC24_002568"
     CDS             8776..9393
                     /gene="ccmA"
                     /locus_tag="JNC24_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HAY9159284.1"
                     /translation="MLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTTLL
                     RLLTGLSRPDAGEVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHRDG
                     DTAQCLEALAQAGLAGFDDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAIDVN
                     GVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            9390..10052
                     /gene="ccmB"
                     /locus_tag="JNC24_002569"
     CDS             9390..10052
                     /gene="ccmB"
                     /locus_tag="JNC24_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HAY9159285.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            10094..10831
                     /gene="ccmC"
                     /locus_tag="JNC24_002570"
     CDS             10094..10831
                     /gene="ccmC"
                     /locus_tag="JNC24_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HAY9159286.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            10828..11037
                     /gene="ccmD"
                     /locus_tag="JNC24_002571"
     CDS             10828..11037
                     /gene="ccmD"
                     /locus_tag="JNC24_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HAY9159287.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            11034..11513
                     /gene="ccmE"
                     /locus_tag="JNC24_002572"
     CDS             11034..11513
                     /gene="ccmE"
                     /locus_tag="JNC24_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HAY9159288.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMLGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            11510..13453
                     /locus_tag="JNC24_002573"
     CDS             11510..13453
                     /locus_tag="JNC24_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HAY9159289.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMIASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASYSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLAIAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSIDIHEYRFTFRDVKEVTGPNWRGGVATIGVTREGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            13450..14007
                     /gene="dsbE"
                     /locus_tag="JNC24_002574"
     CDS             13450..14007
                     /gene="dsbE"
                     /locus_tag="JNC24_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HAY9159290.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            14004..15056
                     /locus_tag="JNC24_002575"
     CDS             14004..15056
                     /locus_tag="JNC24_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein CcmH"
                     /protein_id="HAY9159291.1"
                     /translation="MRFLLGMLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVAYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIDGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(15091..15738)
                     /gene="narP"
                     /locus_tag="JNC24_002576"
     CDS             complement(15091..15738)
                     /gene="narP"
                     /locus_tag="JNC24_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HAY9159292.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRAGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            16058..>16425
                     /locus_tag="JNC24_002577"
     CDS             16058..>16425
                     /locus_tag="JNC24_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HAY9159293.1"
                     /translation="MHQSGSVSLCRSAISVLVATALYSPIALASTVEYGETVDGVGLE
                     KDIQLVYGTANNTKINPGGEQHIKEFGVSSNTEINGGYQYIEMNGTAEYSVLNDGYQI
                     VQMGGAVNQTTLNNGVLQVYG"
BASE COUNT         3577 a         4124 c         4775 g         3949 t
ORIGIN      
        1 tgttaccgtt gatgccgcgc aaatctcacc tgcaataaag cgactaaaag taaggcatta
       61 acaagatgaa aaaagtgact gccatgctct tctcgatggc cgtggggctt aatgccgttt
      121 cgatggcggc aaaagcgaaa gcgtccgagg agcaggaaac tgatgtactg ttgattggcg
      181 gcggcattat gagcgccacg ttggggacct atttacgcga gctggagcct gaatggtcga
      241 tgaccatggt ggagcgcctg gagggtgtcg cgcaggagag ttcgaacggc tggaacaacg
      301 ccggaaccgg gcattctgca ctgatggaac tgaactacac cccgcaaaac gccgatggca
      361 gtatcagtat tgaaaaagca gtcgccatta acgaagcatt tcagatttcc cgccagttct
      421 gggcgcacca ggtcgagcgc ggcgtgctgc gtactccgcg ttcatttatc aataccgttc
      481 cgcatatgag ctttgtctgg ggcgaggata acgttaattt cctgcgcgcc cgttacgccg
      541 cgttgcaaca aagctcgctg tttcgcggta tgcgttactc tgaagatcac gcgcagatca
      601 aagagtgggc accgttagtg atggaagggc gcgatccgca acagaaagtg gcagccacgc
      661 gtacggaaat tggtaccgat gtgaactacg gcgagatcac ccgccagtta attgcttcct
      721 tgcagaagaa atctaacttc tcgctgcaac tcagcagcga agtccgcgcc ctaaagcgta
      781 atgacgataa cacctggacc gttaccgttg ccgatctgaa aaatggcact gcacagaaca
      841 ttcgtgccaa atttgtcttt atcggcgcgg gcggtgcggc gctgaagctg ttacaggaat
      901 cggggattcc ggaagcgaaa gactacgccg gtttcccggt gggcggacag ttccttgttt
      961 cggaaaaccc ggacgtggtt aatcaccatc tggcgaaggt ttacggtaaa gcatccgttg
     1021 gcgcaccacc gatgtcggtt ccgcatatcg atacccgcgt tctggacggt aaacgcgtag
     1081 tgctgtttgg gccatttgcc accttctcaa ccaaattcct caaaaacggt tcattgtggg
     1141 atctgatgag ttccaccacc acctctaacg tgatgccgat gatgcacgtc gggctggata
     1201 atttcgatct ggtgaaatac ttggtgagtc aggtgatgtt gagtgaagag gatcgttttg
     1261 aagcgttgaa agagtactat ccgcaggcga aaaaagagga ctggcgtttg tggcaagcgg
     1321 ggcagcgtgt gcagattatc aagcgtgatg ccgataaagg tggtgtactg cgtctgggta
     1381 ctgaagtggt cagtgaccag caagggacca ttgccgcgct gctgggggca tcgccagggg
     1441 cgtcaaccgc tgcgccgatt atgttggatc tgctggaaaa agtatttggc gatcgcgttt
     1501 ccagcccgca atggcaggct acgttgaaag cgatcgttcc atcttatgga cgcaagctga
     1561 acggtgatgt agcggcaaca gaacgcgagt tgcagtacac cagcgaagtg ctgggcctga
     1621 aatacgacaa gccgcaggca gcagatagta cgccgaaacc gcagttgaaa ccgcaacccg
     1681 ttcaaaaaga agtggcggat attgcgttgt aatgatgtgc cacatccggc atggtatgcc
     1741 ggatgtggcg taaccgcctt atccgaccta cggggtggag cacttcacag cggtgtaggc
     1801 ctgataagac gcgtcagcgt cgcatcaggc aaccggctcg ggcgttagat gtcggatgcg
     1861 gcgtaaccgc cttatccaac ctacggggtg gagcacttca cagcggtgta ggcctgataa
     1921 gacgcgccag cgtcgcatca ggcaaccggc ttgggcgtta gatgtcggat gcggcgtgac
     1981 cgccttatcc gacctacggg gtgacgcact tcacagcagt ttagcgaact accgcgttgt
     2041 caattttctc ttccgccttc cagacgcggt acttcacatc tacattgtct ggcgtataca
     2101 ccacgatcgg cagcttgctg ttgtaacgca gcattccagc atcgcccaga tacgcggtga
     2161 caaatttctt ctctttcttg ccatccgggc aggccatcat cgtggaaacc ggcgagctaa
     2221 ctttatcaaa gacataataa tcatagcccc agccttccag cgttttgttt tccagcttcc
     2281 cgccgagacg atgcaaattg caatcgactt ccagcgtctg accggttaac agttctactt
     2341 tcagggtaga ttcatcttct tgcggggtta actgaatcac ctgacgcttc atcccttttt
     2401 cagcttgtgg ataaggcgcg attttttcca gtggctggac gctttctgcc gcccaggcgg
     2461 aagtggtggc gaaagcggca aacaatactg caggtagaat ggtcttcatt tttttgcttt
     2521 ccttcttcat ttataggttt tcaccgatga tgtcgctgaa ggttaacata ttcatcagat
     2581 ttattaattt gctttatgaa taacttaaag aacattcaga caattttacc tgctctttta
     2641 gtgttaaatt ctgatgaggg agcaaaccag cagaaatatc tctggtgaat taatcgctat
     2701 ataaatatgt ttataaccat ttgaaatgtg agcaaaagcc cgtttttgcc acgcttcccg
     2761 ccactctttt gatcctgcta caggttttac cccgatcggg gtatgcatct ttgacacatc
     2821 ctttaatatc ttagcggcta taaaaatggc ttattaatta tgcggtttat ttggtcgctc
     2881 tcaattttca gagcgcgtta atgatggaag gtcaatgtga agattgatgc atcccgtcgg
     2941 ggcatactca ctggtcgctg gcgcaaagcc agtaacggta tccgtccgcc ctggtccggt
     3001 gatgaatctc attttctgac ccattgcacc cgttgcgacg cctgtattaa tgcttgtgaa
     3061 aacaacattc tgcaacgcgg cgcgggcggc tatccgagcg ttaatttcaa aaataacgag
     3121 tgctgcttct gttacgcctg cgcgcaggct tgccccgaat cgctattttc tccgcgccac
     3181 accagggcct gggatttgca gttcaccatt ggggacgcgt gcctggcgta tcagtcagtt
     3241 gaatgccgcc gctgtcagga tagctgtgag ccaatggcaa ttatctttcg cccgacgctc
     3301 tccgggatct accagccgca acttaatagc caactttgca acggatgtgg agcatgtgca
     3361 gccagctgtc cggtatcagc cataactgcg gagtatctcc atgcacacta actggcaagt
     3421 ttgcagcctg gtcgtgcagg ccaaaagcga acgaatttca gacatcagca cccaactgaa
     3481 cgcctttccc ggctgtgaag ttgctgtcag cgacgcgccg agcggtcagt tgattgtggt
     3541 ggtggaagca gaagacagcg aaacgctgat ccaaaccatt gagtcagtac gcaacgtaga
     3601 gggcgtgctg gcggtgtcgc tggtttatca ccagcaggaa gagcaaggtg aggaaacacc
     3661 atgaaactca gtcgtcgtag ctttatgaaa gctaacgccg ttgcggccgc tgcggcggct
     3721 gccggtctca gcgtgccggg cgttgcccgc gccgttgttg gtcagcagga agccattaaa
     3781 tgggataaag cgccgtgccg tttctgcggt actggttgcg gcgttctggt cggaacgcag
     3841 caggggcgtg tggtggcctg tcagggcgac ccggacgcac cggttaaccg tggcctgaac
     3901 tgcattaagg gctatttcct gcccaagatc atgtacggta aagaccgttt gacgcagccg
     3961 ctgctgcgta tgaaaaacgg taaatatgac aaagaaggcg aatttacccc aatcacctgg
     4021 gatcaggcct tcgatgtgat ggaagagaaa ttcaaaaccg ccctgaaaga aaaagggccg
     4081 gaatcgatcg gtatgttcgg ttctggtcag tggactatct gggaaggtta tgccgcgtcc
     4141 aagctgttta aagcgggctt ccgttcgaac aacatcgacc cgaacgcgcg tcactgtatg
     4201 gcgtcggcag tagttggctt tatgcgtacc tttggtatgg atgagccgat gggctgctat
     4261 gacgacatcg agcaggctga cgcgtttgtg ctgtggggcg caaacatggc ggagatgcac
     4321 ccgatcctct ggtcgcgcat cactaaccgt cgtctctcta atcagaacgt caccgtggcg
     4381 gtgctttcta cctaccagca tcgtagcttc gagctggcgg ataacggcat catctttacg
     4441 ccacagtctg acctggtgat cctgaactac atcgccaact atatcattca aaacaatgcg
     4501 ataaatcagg acttcttcag caaacacgtt aacctgcgca aaggggcgac ggacatcggc
     4561 tacggtttac gtccgaccca tccgctggaa aaagcagcga agaatccagg ttctgacgcc
     4621 tccgaaccga tgagctttga agattacaaa gccttcgttg ccgagtatac gctggaaaaa
     4681 accgctgaaa tgaccggcgt gccgaaagac cagttagaac aactggcgca gctgtatgcc
     4741 gatccgaaca agaaagtcat ctcctactgg acgatgggct tcaaccagca tactcgtggc
     4801 gtgtgggcta acaacctggt ctacaacctg cacctgctga ccggcaaaat ttcccagccg
     4861 ggttgcggtc cgttctccct gaccgggcaa ccttctgcgt gtggtactgc gcgtgaagtg
     4921 ggcacctttg ctcaccgtct gcctgcggac atggtggtga ccaacgagaa acaccgtgat
     4981 atctgcgaga agaagtggaa tatcccgagc ggcaccattc cggcgaaaat cggtctgcac
     5041 gcggtggcac aagaccgtgc gctgaaagac ggcaaactga atgtttactg gaccatgtgt
     5101 accaacaaca tgcaggccgg gccgaacatc aatgaagagc gtatgccggg ctggcgcgat
     5161 ccgcgcaact tcatcatcgt ctccgatccg tatccgacag tcagtgcgct ggccgccgac
     5221 ttgatcctgc cgaccgcaat gtgggtagag aaagagggtg cttacggtaa cgccgaacgc
     5281 cgtactcagt tctggcgtca gcaggtacag gcaccgggcg aagcgaaatc ggatctctgg
     5341 cagttagtcc agttctcccg ccgcttcaaa actgaagaag tgtggccgga agagctgcta
     5401 gcgaagaaac cggaactgcg tggcaaaacg ctgtacgaag tgctttatgc cacaccggaa
     5461 gtgagcaaat tcccggtatc cgaactggcg gaagatcagc tgaacgatga atcccgcgag
     5521 ctgggcttct atctgcaaaa agggctgttc gaagagtacg catggtttgg tcgcggtcac
     5581 ggtcacgatc tcgcgccgtt cgatgactac cacaaagcgc gcggtctgcg ctggccggtg
     5641 gtgaacggta aagaaacgca gtggcgttac agcgaaggta acgacccgta cgtgaaagcg
     5701 ggcgaaggct ataagttcta cggtaaaccg gatggtaaag cggtgatctt cgcgctgccg
     5761 ttcgaaccgg cggcggaagc accggatgaa gagtacgacc tgtggctctc taccggacgc
     5821 gttctggagc actggcacac tggcagtatg actcgccgtg tgccggaact gcaccgcgcc
     5881 ttcccggaag cggtcttgtt tattcacccg ctggatgcga aagcgcgcga tctgcgccgt
     5941 ggcgacaaag tgaaagtggt ttctcgccgt ggcgaagtga tctcgattgt tgaaacgcgc
     6001 ggtcgtaacc gtccgccaca gggcctggtg tacatgccgt tcttcgacgc cgcacagctg
     6061 gttaacaaac taacgctgga cgcgaccgat ccgctctcga aagagacgga cttcaagaag
     6121 tgcgcagtca aactggagaa ggtgtaagcc attatgtccc ggtcagcgaa acctcaaaat
     6181 ggtcgccgcc gctttctgcg cgatgttgtt cgcacagcag gcgggctggc tgccgtgggt
     6241 gtggcgctgg ggttacaaca gcaaaccgca cgcgcatctg gcgtgcggtt gcgcccgccc
     6301 ggagccataa acgagaacgc ctttgccagt gcctgtgtgc gttgcggtca gtgtgttcag
     6361 gcttgccctt acgacacctt aaaactggcg acgctggctt ctggcctgtc ggcgggcacg
     6421 ccatattttg tcgcacggga tattccttgc gaaatgtgtg aggacattcc gtgcgccaaa
     6481 gtgtgcccga gtggtgcgct ggatcgtgag atcgaatcga tcgacgacgc gcggatgggg
     6541 ctggcggtgc tggtggacca ggaaaactgt ctcaactttc aggggctgcg ctgcgatgtt
     6601 tgttatcgcg aatgcccgaa aattgatgag gccatcaccc tggagctgga gcgcaacacg
     6661 cgtaccggta agcacgcccg ctttctgccg acggttcaca gcgacgcctg taccggctgc
     6721 ggtaagtgcg aaaaagtgtg cgtgctggaa caaccggcaa tcaaggtgtt accgctgtca
     6781 ctggcgaaag gggagttagg tcaccattac cgcttcggct ggctggaggg gaacaatggc
     6841 aaatcgtaaa cgtgacgccg ggcgcgaggc gctggtgaaa aaaggctggt ggcgcagtca
     6901 ccgttggctg gtgttacgtc gcctttgtca gttcttcgtg ctggggatgt ttttgagcgg
     6961 tccgtggttt ggtgtgtgga tcttacacgg caactacagc tgtagcctgt tattcgacac
     7021 cgttccgctg accgatccgc tgatgacgct gcaaagtctt gccagtggtc atctgcctgc
     7081 cacggtggcg ttgaccggtg cggtgataat cactgtgctc tacgccctgg caggaaagcg
     7141 attattttgc agctgggtct gcccgctgaa cccgattacc gacttagcga actggttacg
     7201 caggcgcttt gacctcaatc agtctgcgac gatccctcgc cacattcgct acgtgctgtt
     7261 ggtggtcatt ctgctgggat cagccttaac cggcacgctc atctgggaat ggatcaaccc
     7321 cgtttccctg atggggcgta gcctggtaat gggcttcggc agcggcgcgc tgcttattct
     7381 cgcgctgttt ttatttgatc tactggtcgt tgaacacggc tggtgcgggc acatttgtcc
     7441 ggttggcgcg ctgtatggcg tgctgggtag caaaggtgtg attaccgttg cggccagcga
     7501 tcggcagaaa tgtaaccgct gtatggattg ttttcatgtt tgcccggaac cgcatgtgct
     7561 acgtgccccg gtgctggatg aacaaagccc ggtgcaggtc accagccgcg attgcatgac
     7621 gtgcggtcgc tgcgtggatg tctgttctga ggatgtattt acaataacta cacgatggag
     7681 ttcgggagcg aaatcatgaa aagccatgac ctgaagaaag cgctgtgtca atggacggcg
     7741 atgctggccc tggtggtaag cggcgcggtt tgggcggcta atggcgtcga ttttagccag
     7801 tcgccggaag tatccgggac gcaggaaggg gccattcgga tgccaaaaga gcaggatcgg
     7861 atgccgctga actatgtgaa tcagccgcca atgatcccgc atagcgttga aggttatcag
     7921 gtaacgacca ataccaaccg ctgcttgcag tgccacggtg tcgaaagcta tcgcaccact
     7981 ggcgcgccgc gtatcagtcc tactcacttt atggacagcg acggcaaagt gggcgcggaa
     8041 gtggctccgc gtcgttattt ctgtctgcaa tgtcacgtac cgcaggccga taccgcgcca
     8101 atcgtgggga atacctttac cccatcaaaa ggttacggga aataagaggt tattatggga
     8161 aattctgacc gtaagcctgg tctgattaag cgcctgtgga aatggtggcg tacccccagc
     8221 cgtctggcgc tgggaacgct gctgttgatc ggttttgtcg gcggcatcgt cttctggggc
     8281 ggctttaaca ccggaatgga aaaagccaat accgaagagt tctgcattag ctgccacgaa
     8341 atgcgtaaca cggtgtatca ggaatacatg gattccgtgc actacaacaa ccgtagcggc
     8401 gtccgtgcga cctgtccgga ttgtcacgtt ccgcacgagt ttgtgccgaa gatgatacgc
     8461 aagctcaaag caagtaaaga gctgtatggt aaaatttttg gcgttattga cacgccgcag
     8521 aaatttgaag ctcatcgtct gacaatggca cagaatgagt ggcggcgcat gaaggacaat
     8581 aactcgcagg agtgccgtaa ctgtcacaac ttcgagtata tggatacaac cgcccagaaa
     8641 tcggttgccg cgaagatgca tgaccaggcg gtgaaagatg ggcaaacctg tattgattgc
     8701 cataaaggga tagcgcacaa gctgcccgat atgcgtgaag tcgagccagg tttttaacag
     8761 ggttattgcg tgggtatgct tgaagccaga gagttacttt gtgagcggga tgaacgaacc
     8821 ttatttagtg gcttgtcatt tacgctgaac gcaggagagt gggtacaaat caccggtagc
     8881 aacggcgcgg ggaagacaac gcttctccgt ttgctgacgg ggttgtctcg ccctgacgca
     8941 ggcgaggttc tctggcaagg gcagcccttg catcaggtac gcgacagcta ccatcaaaac
     9001 ctgttatgga taggccatca gccggggatc aaaacccggc tgacggcgtt agaaaatctg
     9061 cacttttatc atcgcgatgg cgataccgca caatgtctgg aagccctggc gcaggccggg
     9121 cttgccggat tcgacgatat tccggtaaat caactttctg ccgggcaaca acgccgcgtc
     9181 gctttagcgc gtctgtggct gacccgtgcc acgttatgga tcctcgacga gccttttacc
     9241 gcgattgacg ttaacggcgt cgatcgtctg acccagcgta tggcgcagca tacggagcag
     9301 ggggggattg tgattctgac tacccaccag ccgctcaacg ttgctgaaag taaaattcgc
     9361 cgcatttcac tgacgcaaac gagggccgca tgatgttctg gcgcattttc cgtcttgagc
     9421 tgcgtgtagc gtttcgccat agcgccgaaa tcgccaaccc gctgtggttc ttcctgattg
     9481 taattaccct ttttccgctc agtatcggtc cggagccgca actgctggcg cgtattgcac
     9541 cgggcattat ctgggttgct gcgctgcttt catccttgct ggcgctggaa cgactgttcc
     9601 gtgacgattt gcaggacggc agtcttgaac aattgatgtt gttgccgtta cccttgcccg
     9661 ccgttgtgct ggcgaaggtg atggcgcact ggatggtaac cggtctgccg ttactcatcc
     9721 tttcgccact ggtagcaatg ctactgggaa tggatgttta tggctggcaa gtgatggcgc
     9781 tgacgctgct gctgggaacg cctacgcttg gctttctcgg tgcaccgggt gtggcgttga
     9841 cagtgggact taagcgcggt ggtgtgctgc tcagcatact ggtgttaccg ctgactatcc
     9901 cattactcat ctttgccacc gccgcgatgg acgcggcttc catgcatttg cccgttgacg
     9961 ggtatctggc aattttaggc gcgttgctgg caggcaccgc gacattaagt ccttttgcga
    10021 cggcggcagc gttacgaatc agcattcaat aacgctggat tactgatttt tgttcgagtc
    10081 tggtatcgaa actatgtgga aaacactgca tcaactggcg atcccaccac ggctgtatca
    10141 aatctgtggc tggtttatac cgtggctggc aattgccagt gtggtcgtgc ttaccgtcgg
    10201 ctggatctgg ggattcggct ttgctccggc tgattatcag cagggaaata gctaccgcat
    10261 tatctacctg catgtgcctg cggcgatctg gtcgatgggc atttatgcat caatggcagt
    10321 ggcagcgttt attggccttg tctggcagat gaaaatggcc aacctggcgg tggcggcgat
    10381 ggcccccatt ggtgccgtgt ttacctttat tgccctggtt accggctctg catggggaaa
    10441 accgatgtgg ggcacctggt gggtatggga tgcacgtctg acttctgaac tggtgctgct
    10501 gtttttgtat gtgggtgtga ttgccctgtg gcacgccttc gacgaccgcc gtctggcggg
    10561 ccgtgcggca ggtatcctgg tgctgattgg cgtggtgaat ctgccgatta ttcattactc
    10621 cgtggagtgg tggaacaccc tgcatcaggg atcaacgcgg atgcagcaaa gtatcgatcc
    10681 ggcgatgcgt tcgccgctgc gctggtcgat ttttggcttc ctgctcctgt ctgccacgct
    10741 gacgctgatg cggatgcgta atttgatttt gctgatggaa aaacgccgtc cgtgggtgag
    10801 tgaactgata ctgaaaagag gccgtaaatg acccctgcat ttgcttcctg gaatgaattt
    10861 ttcgcaatgg gcggttacgc cttttttgtc tggctggcgg tggtgatgac cgttattccg
    10921 ctggtggttt tggtcgtgca ctcggtgatg caacatcgcg caattctgcg tggtgtggcg
    10981 caacagcggg cgcgtgaggc gcgtttacgt gctgcgcaac agcaggaggc tgcatgaata
    11041 ttcgccgtaa aaaccgcttg tggattgcct gtgccgtgtt ggcagggctg gcgctgacta
    11101 tcggtctggt gctatatgcg ctgcgctcga atatcgatct cttttatacg ccgggggaaa
    11161 ttctctacgg caagcgtgaa actcagcaaa tgccggaagt cggtcagcgt ttgcgcgttg
    11221 gcgggatggt gatgctgggt agtgtgcagc gcgatcccaa ttcgctgaaa gtgaccttca
    11281 ctatttacga tgctgaaggc tcagtggatg tctcttacga aggcattttg ccggatctgt
    11341 tccgtgaagg gcagggcgtt gtggtgcagg gcgagctgga aaaaggcaat catatcctcg
    11401 cgaaagaagt gctggcgaaa cacgacgaaa actacacgcc gccagaagtt gagaaagcga
    11461 tggaagctaa ccaccgtcgc ccggcgagtg tttataagga cccagcatca tgatgccgga
    11521 aattggaaac gggctgctgt gtctggcgtt agggattgcg ctgctgctgt ccgtgtatcc
    11581 gctatggggc gtggcgcgcg gagatgcgcg catgatcgcg tcttcccgct tgtttgcctg
    11641 gctgctgttt atgtctgtgg ctggcgcatt tctggtgctg gtcaatgctt tcgtggtcaa
    11701 cgactttacc gtcacctatg ttgccagcta ctccaatacc cagcttccgg tgtggtatcg
    11761 cgtggcggct acctggggcg cgcatgaagg ctcgctactg ctgtgggtgc tgctgatgag
    11821 cggctggacc tttgcggtgg cgatttttag tcagcgtatt ccgctggata ttgtggcccg
    11881 cgtactggcg ataatgggga tggtcagcgt cggctttttg ctgttcattc tctttacctc
    11941 taacccattc tcacgcacgc tgccgaactt cccgattgaa gggcgcgatc ttaacccgct
    12001 gttacaggac ccggggctga tcttccatcc gcctctgctt tatatggggt acgtgggttt
    12061 ctcggtggcg tttgcttttg ccattgcttc tttgctgagc gggcgtctgg acagcactta
    12121 tgcgcgtttt actcgcccgt ggacgctggc ggcgtggatt ttcctgacgc tcggcattgt
    12181 gctcggttcc gcatgggcct attacgaact cggctggggg ggctggtggt tctgggatcc
    12241 ggtagaaaac gcctcgttta tgccgtggct ggtggggact gcgctgatgc actcactggc
    12301 ggtcactgaa caacgcgcca gcttcaaagc gtggacatta ctgctggcaa tcagtgcctt
    12361 ctcgttgtgt ctgttgggga ctttcctcgt gcgttccggc gtgctggtat cggtacacgc
    12421 gtttgcgtct gatccggcgc gcggtatgtt tatcctcgcc tttatggtgc tggtgattgg
    12481 aggttcgctg ctgctgtttg ccgcgcgtgg acacaaagtt cgctcacgcg taaacaatgc
    12541 gctgtggtcg cgggaatctc tgctgttagc gaacaacgtt ttgctggtcg ccgcgatgct
    12601 ggtggtgttg ctggggacgc tgctgccgct ggtgcacaag caactgggac tgggcagtat
    12661 ttcgattggc gaaccgttct tcaacaccat gtttacctgg ctgatggtgc cgtttgcgct
    12721 actgctgggt gtcggtcctc tggtgcgctg ggggcgcgat cgcccacgta aaatccgcaa
    12781 tttattgatt atcgccttca tctcaacgct ggtgctgtct ctgctgttgc cgtggctgtt
    12841 tgagagcaaa gttgtggcga tgacggtgct cggcctggca atggcctgct ggattgcggt
    12901 gctggcaatt gcggaagctg cgctgcgtat ttcacgcggc acgaaaacca ccttcagtta
    12961 ttgggggatg gtggctgctc acctggggct ggcagtgaca attgttggca ttgcttttag
    13021 ccagaactat agcgttgagc gtgatgtgcg catgaagtcc ggcgatagca tcgatattca
    13081 tgaatatcgc ttcaccttcc gtgatgtcaa agaggtgact ggcccgaact ggcgtggcgg
    13141 tgtggcgact atcggcgtaa cgcgagaggg taaaccggaa acagtgctgt atgcggaaaa
    13201 acgttattac aacactgccg ggtcgatgat gaccgaagcg gcgattgacg gtggcatcac
    13261 gcgtgacctg tacgccgcgc tcggtgaaga gctggaaaac ggcgcgtggg ctgtgcgtct
    13321 ttactacaaa ccatttgttc gctggatttg ggcgggcggg ctgatgatgg cgttgggcgg
    13381 actgctgtgt ctgtttgatc ctcgctatcg taagcgcgtg agtccgcaaa aaactgcgcc
    13441 ggaggctgta tgaagcgcaa agtattgtta attccgttga ttatcttcct ggcgattgcc
    13501 gcggcgctgc tgtggcagct ggcgcgtaat gccgaagggg atgatccgac caatctggaa
    13561 tcggcgctca ttggcaagcc agtgccgaag tttcgcctcg aatcactgga caatccgggg
    13621 cagttttatc aggcggatgt gctgactcag ggcaaaccag tgctgcttaa cgtctgggcg
    13681 acctggtgtc cgacctgccg tgcggaacat caatatctga atcagctttc tgcgcagggc
    13741 atccgcgtag tcggcatgaa ctataaagac gatcgccaga aggcaatcag ctggctgaaa
    13801 gagctgggca atccttacgc gctaagcctg tttgatggcg atggcatgtt agggctggat
    13861 ctcggtgtct atggcgcgcc agaaacgttc cttattgacg gcaacggcat cattcgctat
    13921 cgccatgcgg gcgatctcaa tcctcgcgtc tgggaagaag agatcaagcc gctgtgggag
    13981 aaatacagta aggaggccgc acaatgaggt ttttattggg catgctgatg ctgatgatct
    14041 ccggctcagc gctggcgacc atagacgtgt tgcagtttaa agatgaagca caggaacaac
    14101 agttccgtca gctcactgaa gaactgcgct gcccgaaatg ccagaacaac agcattgccg
    14161 attccaactc gatgattgcc accgacctgc gccagaaagt gtatgaactg atgcaggaag
    14221 gtaaaagtaa aaaagagatt gtcgattata tggtggcgcg ttacggcaac ttcgtcactt
    14281 acgatccgcc gttaacgccg ctgaccgtgc tgctgtgggt gctgccagta gtagctattg
    14341 gcattggcgg ttgggtcata tacgcccgct cgcggcgtcg ggtacgcgtg gtgccggaag
    14401 cgtttcctga acaaagcgtg ccggaaggta agcgtgccgg atatgtcgct tatttgccgg
    14461 gcattgtggt ggcgttaatt gtggctggcg tcagctacta ccagaccgga aattatcaac
    14521 aggtgaaaat ctggcagcag gccacggcac aggctccggc gttgctggac agggcgctgg
    14581 atccgaaagc tgatccgctc aacgaagaag agatgtcgcg ccttgcgctg gggatgcgta
    14641 ctcaactgca aaaaaatccg ggagatatag acggctggat tatgttgggc cgcgttggca
    14701 tggcgctggg taacgccagt atcgccaccg atgcatacgc tactgcgtat cgcctcgatc
    14761 cgaaaaacag tgatgccgca ctgggttatg ctgaagcgtt gacacgttca tctgatccca
    14821 acgacaaccg cctcggcggc gaactgctac gtcagctggt gagaacggac catagcaata
    14881 tccgtgtgct aagcatgtat gcgtttaatg cctttgagca gcagcgattt ggcgaagccg
    14941 ttgccgcgtg ggagatgatg ttgaaactct tacctgccaa cgatactcgc cgtgcggtga
    15001 ttgaacgtag tatcgcgcag gcgatgcaac atttgtcgcc gcaggagagt aaataaaaag
    15061 aggcccgatg gtgatgccat cgggctattt ttattgtgcc ccgcgttgtt gcaggaacag
    15121 aatggtggcc gccacgcgtg agcggacatt gagtttacgc agcagatttc gaatatgaac
    15181 ttttactgtc tgctcggaaa tattcaacac agaggcaatc tgtttatttg acagcccctg
    15241 tgccagctcg tgcagaacat ccagctcgcg ctccgtcagc acgctgaagg gatcttcttc
    15301 cgcgccaaac atttcacgtt cacgtaagta ctgattgacg cgttcgctaa agactttgct
    15361 gcctttcgct ccggcacgaa tcgcttccag caacacttcc gggtcgctgt ctttcagcag
    15421 ataaccgtct gcgcctgcgt ctatcagcgc aaagacatcg ctggaggcat cggatacggt
    15481 caggataata atttgcgcgg taacgccatc cctgcgcaag gcattgaggg tatccaggcc
    15541 actcatacct ttcatattga gatccagcaa gatcacgtcg atatccagtc tattcgccag
    15601 atcgatcgcg ctcgcgccgt cgcccgcttc ggcgaccact tcaaagccag gatcaagctc
    15661 cagtaactga cgaacaccgc gtcgcataag tggatgatca tccacaatca tcacctgaaa
    15721 aggcgttgct tcaggcatag tagtctcctg aggttttatt agaatcatta tgtttgagcg
    15781 tagcgttggt aacgctacca ggcgcgtgaa tgttacccca ttttatgtat ggacatgtag
    15841 tgaaaactac aaccttgccg ttcgctttgg catcattttc attataaaga cggaattttg
    15901 catcagcttc tcatcaagaa aaacaactaa aaaatcaatc acaatacttg tgcatttctt
    15961 taaacagttt ttaaaaactg actccgggta tggagctatg ggtattttct gtacccaatg
    16021 cttttaacag caattaattt cataggatga aagctcaatg catcaatctg gttctgtttc
    16081 tctttgtcgt tccgcaatat ctgttctggt ggctacagcg ttatattcac ccatagcatt
    16141 ggcatcaaca gttgagtatg gtgagacagt tgatggtgtt ggcctggaaa aagatatcca
    16201 gctggtttat gggaccgcca ataatacgaa aatcaatcct ggcggagaac agcatataaa
    16261 agaatttggt gtaagtagta atactgaaat taacggcggg tatcagtaca ttgaaatgaa
    16321 tggcactgca gaatactcag tattaaatga tggttatcaa attgttcaaa tgggtggcgc
    16381 ggtaaaccag actacgctca ataatggagt gctacaggtt tatgg
//
LOCUS       DADMKF010000087        16295 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.088, whole genome shotgun sequence.
ACCESSION   DADMKF010000087 DADMKF010000000
VERSION     DADMKF010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16295
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.088"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            71..577
                     /locus_tag="JNC24_002578"
     CDS             71..577
                     /locus_tag="JNC24_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005144280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HAY9159294.1"
                     /translation="MGGAAFFVLKGCGDSSDVDNDGDGTFYATVQDCIDDGNNADICA
                     RGWNNAKTAFYADVPKNMTQQNCQSKYENSYYDNVEQSWIPVVSGFLLSRVIRKDRDE
                     PFVYNSGGSSFASRPVWRNTSGDYSWRSGSGKKESYSSGGFTTKKASTVSRGGYGRSS
                     SARGHWGG"
     gene            580..1743
                     /locus_tag="JNC24_002579"
     CDS             580..1743
                     /locus_tag="JNC24_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HAY9159295.1"
                     /translation="MLRHNVPVRRDLDQIAADNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWHASDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRTTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLCKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKSQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIDSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            1827..3452
                     /locus_tag="JNC24_002580"
     CDS             1827..3452
                     /locus_tag="JNC24_002580"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HAY9159296.1"
                     /translation="MHWQTHTVFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQVLCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPTPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTTGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFVEVKGQDRYFDRAVRRLQQQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQ
                     AWCQVMLDTRGGVRLSEQIQNDLLLRATGGVCL"
     gene            complement(3569..3844)
                     /gene="yjfN"
                     /locus_tag="JNC24_002581"
     CDS             complement(3569..3844)
                     /gene="yjfN"
                     /locus_tag="JNC24_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease activator YjfN"
                     /protein_id="HAY9159297.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(3993..4322)
                     /gene="bsmA"
                     /locus_tag="JNC24_002582"
     CDS             complement(3993..4322)
                     /gene="bsmA"
                     /locus_tag="JNC24_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HAY9159298.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            4504..5253
                     /gene="yjfP"
                     /locus_tag="JNC24_002583"
     CDS             4504..5253
                     /gene="yjfP"
                     /locus_tag="JNC24_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HAY9159299.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDEARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNNIIAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            complement(5250..6005)
                     /gene="ulaR"
                     /locus_tag="JNC24_002584"
     CDS             complement(5250..6005)
                     /gene="ulaR"
                     /locus_tag="JNC24_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HAY9159300.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLD
                     ERGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(6113..7177)
                     /gene="ulaG"
                     /locus_tag="JNC24_002585"
     CDS             complement(6113..7177)
                     /gene="ulaG"
                     /locus_tag="JNC24_002585"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HAY9159301.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            7532..8930
                     /gene="ulaA"
                     /locus_tag="JNC24_002586"
                     /pseudo
     CDS             7532..8930
                     /gene="ulaA"
                     /locus_tag="JNC24_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
     gene            8946..9251
                     /gene="ulaB"
                     /locus_tag="JNC24_002587"
     CDS             8946..9251
                     /gene="ulaB"
                     /locus_tag="JNC24_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HAY9159302.1"
                     /translation="MTVRILAVCGNGQGSSMIMRMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKAHFPQDVK"
     gene            9261..9725
                     /gene="ulaC"
                     /locus_tag="JNC24_002588"
     CDS             9261..9725
                     /gene="ulaC"
                     /locus_tag="JNC24_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HAY9159303.1"
                     /translation="MKLRDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            9739..10389
                     /gene="ulaD"
                     /locus_tag="JNC24_002589"
     CDS             9739..10389
                     /gene="ulaD"
                     /locus_tag="JNC24_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HAY9159304.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            10399..11253
                     /locus_tag="JNC24_002590"
     CDS             10399..11253
                     /locus_tag="JNC24_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HAY9159305.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLRLAKTLGFDFVEMSVDETD
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVVKARDWVKARMA
                     KAGTVEAA"
     gene            11253..11939
                     /locus_tag="JNC24_002591"
     CDS             11253..11939
                     /locus_tag="JNC24_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HAY9159306.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATYLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGDAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSFLMNKHFMRK
                     HGPNAYYGQK"
     gene            12036..12587
                     /locus_tag="JNC24_002592"
     CDS             12036..12587
                     /locus_tag="JNC24_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1440 domain-containing protein"
                     /protein_id="HAY9159307.1"
                     /translation="MAINGLFVRTDPQRRRYGVALLVGVLSGIISAFVKWGAEVPLPP
                     RTFSGGRDEFNPPYIFLRDYLGIDPTQTVYTFSEHIINPVMVTHIIFSLVFAIGYCIV
                     AEIFPKVKLWQGILAGLIVTVAVHGIFCPALNLTPPLTQLPFDEYASEILGHIFWFWI
                     IEIMRRDLRNRITHEPDPEVAIR"
     gene            complement(12662..12977)
                     /locus_tag="JNC24_002593"
                     /pseudo
     CDS             complement(12662..12977)
                     /locus_tag="JNC24_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
     gene            13304..13699
                     /gene="rpsF"
                     /locus_tag="JNC24_002594"
     CDS             13304..13699
                     /gene="rpsF"
                     /locus_tag="JNC24_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HAY9159308.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            13706..14020
                     /gene="priB"
                     /locus_tag="JNC24_002595"
     CDS             13706..14020
                     /gene="priB"
                     /locus_tag="JNC24_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001315977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HAY9159309.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            14025..14252
                     /gene="rpsR"
                     /locus_tag="JNC24_002596"
     CDS             14025..14252
                     /gene="rpsR"
                     /locus_tag="JNC24_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HAY9159310.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            14294..14743
                     /gene="rplI"
                     /locus_tag="JNC24_002597"
     CDS             14294..14743
                     /gene="rplI"
                     /locus_tag="JNC24_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HAY9159311.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(14855..15826)
                     /locus_tag="JNC24_002598"
     CDS             complement(14855..15826)
                     /locus_tag="JNC24_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HAY9159312.1"
                     /translation="MKIKVSAGIIGAVLMLSASQSWAVTLKLSHNQDKSHPVHKAMEF
                     FAKKNKEYSNGDITIRIYPNGTLGTQRETMELIRSGAIPLVKTNAAEMEAFENSYKLF
                     SLPYLFRDRDHYYQVMQGDIGRKILDSTKSKGYFGLTFYDGGARSFYGNKPVLKPDDL
                     KGMKVRVQPSPGAVEMIKVMGGNPTPLDYGELYTALQQGVVDMAENSVMALTTMRHGE
                     VAKSFSLDEHTMVPDVVLMSNAAFDKLSPENQAVILKAAKESMSYMKDLWSEEEKQEF
                     AKLDKMGVKVYQVDKAPFIEKVQPMYANFAKDNPALAPMLADIQAAK"
     gene            complement(15879..>16295)
                     /locus_tag="JNC24_002599"
     CDS             complement(15879..>16295)
                     /locus_tag="JNC24_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005155932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HAY9159313.1"
                     /translation="SWAMSNADVPFLILDLLNTISDNPIIILLIINIILLIIGTFMDM
                     TPAVLIFTPIFLPVVTELGMDPIHFGIVMVLNMCIGICTPPVGSVLFVGCSVSKLPIN
                     KIIKPMLPFYAVMVLVLAMVTYIPQISMALPKALGY"
BASE COUNT         3826 a         3981 c         4334 g         4154 t
ORIGIN      
        1 tggtagaccg aataatcctt ttgaaccgcg tcgcaatcgc tacgcacaaa aatacttaac
       61 gttggcgcta atgggcggtg ccgctttttt cgtattgaaa ggttgtggcg atagtagcga
      121 tgtcgataac gacggcgacg gaacgtttta cgcgacagtg caggattgta ttgatgacgg
      181 taataatgcc gacatttgcg cgcgtggctg gaacaacgcc aaaacggcat tttatgccga
      241 tgttccgaag aatatgactc agcagaactg tcagtctaag tacgaaaatt cctactacga
      301 caatgttgaa cagagttgga tcccggtcgt ttctggattt ttgttaagcc gggttattcg
      361 taaagatcgc gatgagccgt tcgtctataa cagcggcgga tcctcatttg cttcccgccc
      421 cgtctggcgc aacacttccg gtgattactc ctggcgctcc ggttctggca aaaaagagtc
      481 ttactcttca ggcggcttta ccaccaaaaa agcgtcaacc gtttcgcgcg gcggctatgg
      541 tcgttcttcc agcgcccgtg ggcattgggg aggctaatca tgctgagaca caacgttcct
      601 gtgcggcggg atctggacca aattgccgct gacaacggtt tcgactttca tatcatcgac
      661 aatgaaatct attgggacga gagtcgggct taccgtttta ctctgcgcca gattgaagag
      721 cagatcgaaa aaccaactgc ggaactgcat cagatgtgcc ttgaggtggt ggatcgcgcg
      781 gttaaagacg aagagatcct gacgcaactg gcgatcccgc cgttgtactg ggatgtgatc
      841 gccgaaagct ggcacgccag cgatccttcg ctgtatggcc ggatggattt tgcctggtgt
      901 ggcaatgcgc cggtgaagct gctggagtac aacgccgata cgccgacttc gttgtatgag
      961 tcggcttatt tccagtggtt gtggctggag gatgcccggc gcagcggcat tattccgcgt
     1021 gatgccgatc agtacaatgc cattcaggaa cgcctgattt cacgctttag cgagctttat
     1081 agccgggagc cgttttattt ctgctgctgt caggacaccg atgaagacag gactaccgtg
     1141 ctgtacttgc aggactgcgc ccagcaggca gggcaggagt cgcggtttat ctacattgaa
     1201 gatctcggtt tgggcgtcgg cggtgtactg accgatcttg atgataatgt catccagcgt
     1261 gcatttaagc tgtatccgct ggagtggatg atgcgtgacg ataacggtcc gctgctgtgc
     1321 aagcgtcgcg agcaatgggt ggagccgtta tggaaaagta tcttgagtaa taaagggcta
     1381 atgccgctgc tttggcgctt cttccctggt catcctaacc ttcttgcatc ctggtttgag
     1441 ggtgaaaaat cgcagattgc cgctggcgaa agctatgtgc gtaaaccgat ctactcgcgc
     1501 gaaggcggaa acgtcaccat tttcgacggt cagaataacg tcgttgacca cgctgatggt
     1561 gattacgccg atgaaccgat gatctaccag gcgtttcaac ctctgccgcg atttggcgat
     1621 agctacacac tcatcgatag ctggattgtc gatgatgaag cgtgcggaat ggggatccgt
     1681 gaagataaca cactgatcac caaagacacc tcacgtttcg ttccgcatta cattgctgga
     1741 taagaatgtt ttagcaatct ctttctgtca tgaatccatg gcagtggcca tactaatggt
     1801 gactgccatt gatggaggga gacacagtgc actggcaaac tcacaccgtt tttaatcaac
     1861 ctataccatt aaataacagc aacttatacc tgtctgatgg cgcgctttgc gaagcggtaa
     1921 cgcgtgaagg tgctggctgg gatagcgatt ttctagccag tattggtcag cagttaggaa
     1981 cggctgaatc ccttgaactg gggcggctgg cgaatgtaaa cccgcctgaa ctgttgcgct
     2041 acgatgccca ggggcggcgt ctggacgatg ttcgtttcca tcccgcatgg cacctgctga
     2101 tgcaggtgtt atgtaccaat cgggtgcaca atcttgcctg ggaagaagac gctcgctccg
     2161 gcgcatttgt ggcgcgcgcg gcgcgtttta tgttacatgc gcaggttgag gctgggtcgt
     2221 tatgtccaat aacgatgacc tttgccgcca cgccattgtt gttacagatg ttacccacgc
     2281 cgtttcagga ctggaccacg ccgctgctga gcgatcgcta cgattctcac ttattgccag
     2341 gtgggcaaaa acgcggtttg ttgattggca tgggaatgac ggaaaagcag ggtggttccg
     2401 atgtcatgag caacaccacc cgtgcagagc gtttggaaga tggctcttat cggctggtgg
     2461 ggcataaatg gtttttctcg gttccgcaaa gcgatgcgca tctggtgctg gcgcagacca
     2521 cgggcggtct gtcctgtttt tttgtgccgc gctttttgcc tgacgggcaa cgcaacgcga
     2581 ttcgcctcga acggctgaaa gataagctgg gtaatcgctc taacgccagt tgtgaagtgg
     2641 agtttcagga tgccattggc tggttattgg ggcaggaagg ggaaggaatt cgtctgatcc
     2701 tgaaaatggg ggggatgacg cgttttgatt gcgccctggg tagccatgcc atgatgcgcc
     2761 gtgcgttttc gctggcgatt tatcatgcac atcaacgcca tgtttttggt aatccattga
     2821 tccaacagcc ccttatgcgt catgtcttaa gtcgcatggc acttcagctt gaagggcaaa
     2881 cggcgttgct gtttcgtctt gcgcgagcgt gggaccggcg cgccgatgcc aaagaagccc
     2941 tgtgggcgcg tttatttacg cctgcggcaa aatttgtgat ctgcaaacga ggtatgccgt
     3001 ttgtggccga agcgatggag gtgctgggcg gcattggtta ttgcgaggag agcgagctgc
     3061 cgcggcttta ccgggagatg ccggtaaaca gtatttggga aggttccggc aatattatgt
     3121 gcctggatgt gttgcgcgtt ctcaataagc aagcgggcgt atacgactta ttgtcggaag
     3181 catttgtgga agtgaaaggg caggatcgct attttgatcg cgcggttcgt cgtttacagc
     3241 agcagctgcg taagccagct gaagaactgg ggcgagagat tactcatcag ctattcctgc
     3301 tgggctgcgg tgcgcaaatg ttgaaatatg cttctccgcc aatggcgcag gcgtggtgtc
     3361 aggtgatgtt agatacgcgc ggcggcgtac ggttgtcaga gcagatccag aatgatttat
     3421 tgctgcgggc gacgggggga gtgtgtttgt aaacgtatac gactgatgcg acgctggttt
     3481 cgattaactc aatgaaatat gtgaaaattg taggccggac aaggcgctcg cgccgcatcc
     3541 ggcattgttc atttgctgta gatgacactc atgcatacag tatcgcctgt acgcgccagt
     3601 tatcaggctg ctggtcttcg tacatggtaa taatgcggta ccatgacgca ccttgttcat
     3661 cggcctttaa cgccacgata cgttccacgt cctgcggatc ccctgaaata ttgctaactg
     3721 agatctgacc tatttcattc aggcccgtca cgcagtcagc actggcgaat tctgcagact
     3781 gtgccgtggc gctcgcagga taagtcacta attgcagcga gggtgaggca agtaattgtt
     3841 tcatcgtcag ctccattttt cgtttcccca catgttgcag ggcattccat cgcggtaata
     3901 aacgcccagg cttccagccg gtaatttatt gtgcacaggt aaacgcatag ggacgcaaag
     3961 caatgtaaag ctgactatgt aagttgtctg atttatttac ggtacaaaat ggcttgtgaa
     4021 taccactgtc ctgtcacgat ggtttcgtcc accatgacga caacgtaata atcagctttt
     4081 gcagcgacag cttttgctct gatttctgct aatgcgtcat ccggagaacc ccgaaccatc
     4141 gtgcttacgc tacctattcg ctgtaaccct tgcgtctggt cgcggcgaat ctcttgcgga
     4201 tggtccgtta ctggcggtgc tggctgtggc gtaccttgca gtgcgctaca ggcacttaac
     4261 atcaacacca ataataaact ggcaaaccgg taaataacgc tattacgttt cctgctaacc
     4321 atagtgtagt gccttattaa tttaactttg ggggaatgtt cccgaattgt tacctggtac
     4381 gcgattttcg aatgaaaacg tgacgaaagc gtaacccaca tctcaacatt atgaactaat
     4441 accgccagca gaaacttgtg ctaaggattt cttcatgtca taccagacaa aaaggagaga
     4501 cagatgattg aaatagaatc acgcgagctg gcagatattc ccgttcttca tgcttatcct
     4561 gtcgggcaaa aagatacccc gttaccgtgc gtaatttttt atcacggctt tacttcatcc
     4621 agcctggtgt atagctattt tgccgttgcg ctggcgcagg ctggtttgcg ggtgatcatg
     4681 ccggatgcgc ccgatcacgg tagccgtttt agtggcgacg aagcgcggcg gttaaatcaa
     4741 ttctggcaaa tcttgctaca aagtatgcag gaattcacta ctttacgtgc ggcaatagcc
     4801 gaagaaaact ggctgcttga tgaccgtctg gcagtcggtg gcgcgtcgat gggcgcgatg
     4861 acggcactgg gaattaccgc tcgccacccc acggtgaaat gtaccgccag catgatggga
     4921 tcgggctatt ttacatcgct cgcccgttca ctgtttccac cgctgatacc tgaaacggca
     4981 gcacagcaga atgaattcaa taacattatc gcgccgctgg cagagtggga agcgacaaac
     5041 cacctggaac aacttgggga cagacctcta ctgctgtggc atggcctcga cgatgacgtt
     5101 gtgcctgccg acgaatcact acgtttgcag caggccttaa gcgagacggg acgggataaa
     5161 ctgctaacct gttcatggca gccaggcgtg cgtcaccgca ttacgcctga ggcgttagat
     5221 gctgccgtga catttttccg ccagcatctt taaacacgca gaatgctgac cccttgcgct
     5281 tccagttgtt gcaggatttc cgggttagca tttttgccgg tgataagcat atcgatttga
     5341 tcggcacggc taaaaagcat tcccgcgcgt tcgccaatct tactgctatc aaccagtacc
     5401 accagtttcc ctaccacgct cagcatcttc tgctctgcca ttgctgtcag catatcggtt
     5461 ttatacaacc cttctgcggt cagccctttt ccgctggtaa acatccagtg cccggcatag
     5521 agactgtttt cgctgccctg cgggcttaaa gtgatggact gacttttgtt gtactgtcca
     5581 cccataatga tcacgctgtc atgttcttga tcgatcaggt aatttgccag cggtagataa
     5641 ttagtgatga tttgcactgg cttgccacac atttcccgcc caagcagaaa cgcggtggag
     5701 ccgcagttga tgactacgct ttcgcccgga ttaaccagct gcgaggccgc tttagcaata
     5761 cgtacttttt catcgtgatt ctgcgcctga tgcagattca tcggcgtcca gcgcgggcgc
     5821 tgttgggtaa tagcttctgc gccattgcgc acctttttca gtttgccgcg ttcgtcaagt
     5881 ttattgatat cgcgtcgcgc agtggcaggc gaaattccca gacgctcaac gactttctca
     5941 acggtcacaa agcccaattg tgcgagcatt tccaggagga tttgatgtct ttgtgcttca
     6001 gtcatgagct attccgataa aatttgattt ttttagatga tatttgaaat agctaggaaa
     6061 tactacgccg gatagcgcga acttctccac gctatccggt ataaacgaca gattacagga
     6121 atgacttgaa cggcagatcc ggttcaatag tgaagcaatc atcgaaaccg cgcggatagt
     6181 ggtactcgaa gttgtcttta tccagcggcc aggtaaattt gccgccaacc tgccagataa
     6241 acggcttgaa gccatacttc agacgatctt ttttcatctc ccacagcacg cggatctctt
     6301 gcggatcggc ctggaagttt gaccagatgt cgtggtggaa cgggataacc actttcgcat
     6361 tcagggcttc acccatacgc agcatatcgg cgctggtcat tttgtcggta ataccgcgcg
     6421 ggttctcacc atacgatcct aacgccacat cgatctgatg ttcgttaccg tgcttcgcat
     6481 aatagttaga gtagtgggaa tcgccactgt gatataggga accgccaggg gttttgaaca
     6541 ggtagtttac tgcgcgatcg tccatgccat ctggcagtac gccagccgct ttttgatcgg
     6601 caggcagggt gatcagtgca gtacggtcga aagcgtcaag cgcatgaatt tcaatgtctt
     6661 tcacttttac aacatcgccc ggtttgacca cgatgcaacg ctctttcggt acgccccagc
     6721 caatccacaa atcaacgcag gttttcggtc caatgaatgg tacgtcatca gcacagttct
     6781 gcatcacggc agcagcgacg ttaacgtcga tatgatcgtt gtgatcgtga gtcgccagta
     6841 ccgcgtcgat ctggcgaatc gcaaacggat caagaacaaa cggggtggtg cgtaggtttg
     6901 gctgcagttt tttcacgcca gccatgcgct gcatctggtg accctgtttc attaacgggt
     6961 taccgtgact ttgtttgcca gtgccgcacc agaaatcaac gcaaacgttg gtgccacctt
     7021 ctgatttcaa ccagatcccg gtgcagccta gccaccacat cgcaaatgtg ccaggggcga
     7081 cctgctcttg ttcaatttct tcattcagcc agctacccca ctccgggaaa gtgctcagga
     7141 tccaggattc tcgggtgata cttttcactt tactcatcgc catttacctt catgatagtt
     7201 caattcgaat caatatgtga ttggttttga ttaatcctga cactattttt tcaggaaggc
     7261 aatgaccatt ttttgacttt ttgccaggga agttgttgtt gattttgagt gtggaaagat
     7321 ttaatggaat gtgtagttaa attaactaaa tgaatttaaa taaataattg tttcgttgtg
     7381 tgaatcccac tctatccgtg tggaattatt tgcgggtcgc gtcacattta atcataaata
     7441 atcttgttgt gattactttt gaaaattaga gtgagtgcac aacattccgg gtgtgcggaa
     7501 tacccggtta cctcttcttc aggagatcgt tatggagatc ctctacaaca tctttaccgt
     7561 gttttttaac caggtcatga ccaatgcccc gttgttgctg ggtattgtga cctgtctggg
     7621 ctacatccta ctgcgcaaaa gtgtcagcgt tattattaaa ggcacgatta aaaccataat
     7681 tggtttcatg ttgttgcagg cagggtccgg catcctcacc agcaccttca aaccggtggt
     7741 ggcgaaaatg tccgaagtct acggcattaa acggcgcaat ttccgatact tacgcgtcga
     7801 tgatggcaac catcgaccgc atgggcgatg cctatagctg ggtaggttac gcggtgttgt
     7861 tagcgctggc gctgaacatc tgttacgtgc tgctgcgtcg cattaccggt attcgtacca
     7921 tcatgctgac tggtcatatc atgttccagc aggcggggct gattgccgtt acgttgttta
     7981 ttttcggcta ctccatgtgg acaaccatta tctgcacggc gattctggtt tcgctctact
     8041 ggggcattac ctccaacatg atgtacaagc cgactcagga agtgacggac ggctgcggtt
     8101 tctccatcgg tcaccagcaa cagtttgcat catggactgc ctataaagtt gcgccgttcc
     8161 tcggcaaaaa agaggaaagc gttgaagacc tcaaactgcc aggctggctg aatattttcc
     8221 acgacaacat cgtctccacg gcgattgtca tgaccatctt ctttggtgcc attctgctct
     8281 ccttcggtat cgacaccgtg caggcgatgg caggcaaagt gcactggacg gtctatatcc
     8341 tgcaaaccgg tttttccttc gcggtggcga tcttcatcat cactcagggt gtgcgcatgt
     8401 ttgtggcgga actctctgaa gcgtttaacg gtatctccca gcgcctgatc cctggtgcgg
     8461 ttctggcgat tgactgtgcg gctatttata gcttcgcgcc gaacgccgtg gtctggggct
     8521 ttatgtgggg caccatcggt cagctgattg cggttggcat cctggtcgcc tgcggttcct
     8581 cgagcctgat tatcccaggc tttatcccga tgttcttctc caacgccacc atcggcgtgt
     8641 tcgctaacca cttcggcggc tggcgtgcgg cgctgaagat ttgtctggtg atggggatga
     8701 tcgaaatctt tggttgcgtc tgggcggtga aacttaccgg tatgagtgcc tggatgggga
     8761 tggcggactg gtcgattctg gcaccgccga tgatgcaggg cttcttctcc atcggtatcg
     8821 cctttatggc cgtcatcatt gtaattgcac tggcttatat gttcttcgct ggccgcgcac
     8881 tgcgcgcaga agaagatgca gaaaaacaac tggcagaaca gtctgcttaa taaggagttt
     8941 tgattatgac cgtacgtatt ctggctgtgt gtggcaacgg acaaggcagt tccatgatca
     9001 tgaggatgaa agtggaccag tttttaaccc aatcaaacat tgaccacacg gtaaatagct
     9061 gcgcggttgg cgaatacaaa agcgagttga gcggcgcgga tatcatcatc gcttctacgc
     9121 acattgcggg cgaaatcacc gtgaccggca acaaatacgt ggttggcgtg cgcaacatgc
     9181 tctctcctgc cgactttggc ccgaaactgc tggaagtgat caaagcgcat ttcccgcagg
     9241 atgtgaagta aggacgcgcc atgaaattac gtgattcgct ggcggagaat aaatccatcc
     9301 gcctgcaggc tgaagctgag acatggcagg acgcagtaaa aatcggcgtt gacctgctgg
     9361 tggcggcgga tgttgtcgag ccgcgttact accaggcgat tctggatgcg gttgaacagc
     9421 acggtccgta ctttgtgctc gcaccaggcc tggcaatgcc gcacggtcgc ccggaagagg
     9481 gcgttaagaa aaccggtttt gcgctggtga cgctgaaaaa gccgctggag ttcaaccacg
     9541 aagataacga cccggtggat atcctcatca ccatggcggc ggtcgatgcc aacacgcacc
     9601 aggaagtggg catcatgcag atcgtcaacc tgtttgaaga tgaagaaaat ttcgaccgtt
     9661 tacgtgcctg ccgtaccgag caggaagtac tggatctcat tgaccgcacc aacgcggcag
     9721 cttaagaagg aattgaagat gtcattaccg atgttgcaag tcgcgctgga caaccagact
     9781 atggatagcg cctacgaaac cactcgcctg attgccgaag aagtcgacat tatcgaagtg
     9841 ggtaccattc tgtgcgtggg cgaaggcgtg cgtgcggttc gcgatctgaa agcgctctac
     9901 ccgcacaaaa tcgtgctggc agacgccaaa attgccgatg cgggcaaaat tctttctcgt
     9961 atgtgcttcg aagccaacgc tgactgggtg accgtgattt gttgtgcgga tatcaacacc
    10021 gccaaaggtg cgctggacgt agcaaaagag tttaacggcg acgtgcagat cgaactgacc
    10081 ggttactgga cctgggaaca ggcgcaacag tggcgcgacg caggtattca gcaggtggtt
    10141 tatcaccgca gccgtgacgc gcaggccgct ggcgtggcgt ggggtgaagc cgatatcact
    10201 gcgatcaaac gtctttccga tatgggcttc aaagtcaccg tcaccggtgg cctggcgctg
    10261 gaagatctgc cgctgttcaa aggcattcca attcatgtgt ttatcgccgg tcgcagcatc
    10321 cgtgatgccg cttctccggt tgaagccgcg cgtcagttca aacgttccat cgccgaactg
    10381 tggggctaag gagcggatat gttgtccaaa caaatcccgc ttggcatcta tgaaaaagcg
    10441 ctccccgccg gggagtgctg gctggaacgc ctgcgactgg caaaaacgtt gggcttcgat
    10501 tttgtcgaga tgtcggtaga tgaaactgac gagcgcctgt cgcgcctcga ctggagccgc
    10561 gagcagcgtc tggcgctggt gaatgcgatt gttgaaaccg gcgtgcgtgt gccgtccatg
    10621 tgcctttctg cccatcgtcg tttcccgttg ggcagcgaag atgacgcggt gcgggcgcag
    10681 gggctggaga ttatgcgtaa agccatccag ttcgcccagg acgttggcat tcgcgtgatc
    10741 cagctggcgg gctatgacgt ttactatcag gaagccaata acgaaacgcg tcgtcgtttc
    10801 cgtgacggcc tgaaagagag cgttgagatg gcgagccgcg cgcaggtgac gctggcgatg
    10861 gagatcatgg attatccgtt gatgaactcc atcagcaagg cgctgggtta cgcgcactat
    10921 ctcaacaacc cgtggttcca gctctacccg gatatcggca acctgtcggc gtgggacaac
    10981 gatgtgcaga tggagttgca ggccggaatc gggcatatcg tcgcggtgca tgtgaaagac
    11041 accaaaccgg gcgtcttcaa aaacgtgcca tttggcgaag gtgtggtgga tttcgaacgt
    11101 tgcttcgaaa cgctcaaaca gagtggctat tgcgggccgt acctgattga gatgtggagc
    11161 gaaacggcgg aagacccggc ggcagaagtg gtgaaagcgc gtgattgggt gaaagcgcgc
    11221 atggccaaag caggcacggt ggaggcggca taatgcaaaa gctaaaacag caggtgtttg
    11281 aagccaacat ggatctgccg cgctatgggc tggtgacctt tacctggggc aacgtcagcg
    11341 ctatcgaccg cgaacgcggg ctggtggtga tcaagcccag cggcgttgcc tacgaaacca
    11401 tgaaagcaga cgatatggtg gtggtcgata tgagcggcaa ggtggtggaa ggggagtatc
    11461 gcccgtcttc cgacaccgcg acgtatctcg aactctaccg tcgttacccg tcgcttggcg
    11521 gcattgtcca tacccactcc actcatgcta ctgcatgggc gcaggcgggg ctggcgatcc
    11581 cggcgttagg cactacgcac gccgactact tctttggcga catcccgtgt acgcgcggat
    11641 taagcgaaga agaggtgcag ggcgagtatg aactgaacac cggcaaagtg attatcgaaa
    11701 cgctgggtga cgccgagccg ctgcatacgc cggggattgt ggtgtatcag cacggtccgt
    11761 tcgcctgggg gaaagatgcc cacgacgcgg tgcataacgc ggtggtgatg gaagaagtgg
    11821 cgaaaatggc gtggattgcg cgcagtatta acccacaact gaatcacatc gacagcttcc
    11881 tcatgaataa acacttcatg cgtaagcacg gtcctaacgc ctattacggg cagaagtaag
    11941 cagtgcgccc cggaattatt acctcctgcg ggatgtaatt ggtctgattc cggggccgct
    12001 tcagttaaaa aatcgtataa acaacaggtg tttttatggc tatcaatgga ttgtttgtca
    12061 gaacggatcc gcaacgtcgc cgctatggcg ttgcgttact tgtgggtgta ctttcaggaa
    12121 ttatttcagc atttgttaaa tggggagcgg aagtcccctt gccaccgcgc acattttccg
    12181 gtggtcgcga tgaatttaac ccgccgtata tctttctacg ggattattta ggtatcgacc
    12241 caacccaaac tgtgtataca ttctcggaac atattatcaa cccggtgatg gtgacgcata
    12301 tcatcttctc actggttttt gcgatagggt attgtattgt cgcggaaatt ttcccgaaag
    12361 tgaaattatg gcaggggata ttagcagggc ttattgtgac agtggcagtc cacggtatat
    12421 tttgcccggc gctgaattta acgccgccgc tcactcaatt gccttttgac gaatatgcct
    12481 ctgaaatcct ggggcatatt ttctggttct ggataataga aattatgcgc cgcgacttac
    12541 gtaatcgcat tactcatgag ccagatccgg aagtcgcaat ccggtgaata accgcataaa
    12601 aaagccccct gcgaaggggg caaagcaaac tatggcaatg tttcgttggt tatacctggt
    12661 gctagcgata aatatccgcg ctggcgtgca tattgccgtt actccccggt tcccgcatca
    12721 gaattacgtg gtagtacgtt actccccggt tcccgcatca gaattacgtg gtagtacgtt
    12781 gcgccctgcg catctgtctc ttcatttaat gcctgacgcg cttcgctttc agtggcgaaa
    12841 ttatgattga tataaatcac gcctaagctt tgcacatcat ccatgtttct ggcctggtgg
    12901 ttattaattt caatggctgc ccatgtattt gcacttagca aaagcacagc cagaagggct
    12961 aaaactcgac tgaacataga tacctcctcg acggctgact ttgtgtgctc tcctctgtga
    13021 tgatcttctg atttaatttt aatcaatgat aaagaagttg atggtgacca tttctgatgc
    13081 agttgttcaa aaaaacacca tgatgaagtg tgatgaactc caaatcagcg gtttagaggt
    13141 taattgcgaa aggggagatt tatttcggct ctgcccttga gtttagcggg gcatacaagt
    13201 actataacgc gtcatttttc agccgacctt taacacgttc cttgcctccc cgggattcgg
    13261 ctgacccaga caggaggctg aataatccgt aaggagcaat tcgatgcgtc attacgaaat
    13321 cgtttttatg gtccatcctg accagagcga acaggttccg ggcatgatcg agcgctacac
    13381 tgctgccatc actggtgcag aaggcaagat ccaccgtctg gaagactggg gccgccgtca
    13441 gctggcttac ccgatcaaca aactgcacaa agcacactac gttctgatga acgttgaagc
    13501 tccgcaggaa gtgatcgatg agctggaaac taccttccgc ttcaacgatg ccgttatccg
    13561 cagcatggtt atgcgtacca agcacgctgt taccgaagca tctccgatgg ttaaagcgaa
    13621 agacgagcgc cgtgagcgtc gcgatgattt cgcaaacgaa accgctgatg atgctgaagc
    13681 tggggattct gaagagtaat ttctgatgac caaccgtctg gtgttgtccg gcaccgtgtg
    13741 caggactccc cttcgaaagg tcagtccatc aggaattcct cactgccagt tcgtgcttga
    13801 gcatcgttct gtgcaggagg aagccggttt tcaccggcag gcgtggtgcc aaatgcccgt
    13861 tattgttagc ggacacgaaa accaggccat tactcacagt ataacggtcg gcagtcgcat
    13921 aaccgttcag gggttcattt catgccacaa ggcaaagaac ggactgagca aaatggtttt
    13981 gcatgccgag cagattgaat tgatagattc tggagactag ccatatggca cgttatttcc
    14041 gtcgtcgcaa gttctgccgt ttcaccgcgg aaggcgttca agagatcgac tataaagata
    14101 tcgctacgct gaaaaactac atcaccgaaa gcggtaagat tgtcccaagc cgtatcaccg
    14161 gtacccgtgc aaaataccag cgtcagctgg ctcgcgctat caaacgcgct cgctacctgt
    14221 ccctgctgcc gtacactgat cgccatcagt aatcggtcac ggtccattaa tacgactttg
    14281 agaggataag gtaatgcaag ttattctgct tgataaagta gcaaacctgg gtagcctggg
    14341 tgatcaggta aacgttaaag cgggctatgc tcgtaacttc ctggtaccgc agggtaaagc
    14401 tgttccagct accaagaaaa acattgaatt cttcgaagca cgtcgcgctg aactggaagc
    14461 taaactggct gaagttctgg cagctgctaa tgctcgcgct gagaaaatca atgcactgga
    14521 aactgttacc atcgcgtcta aagctggcga cgaaggtaaa ctgttcggtt ccatcggtac
    14581 tcgcgacatc gctgacgctg taactgcagc tggcgttgaa gtggctaaga gcgaagttcg
    14641 tctgccgaac ggcgttctgc gtaccactgg cgagcacgaa gtgagcttcc aggttcacag
    14701 cgaagtattc gcgaaagtga tcgtaaacgt agtagctgaa taattcgtta ttcaacgaga
    14761 cgtaaaaacg ccgaccagtg gtcggcgttt tgcttttgta gcgagcactt gcagatatcg
    14821 ataagggcca caataagcag ccctacgaag ataattactt agctgcctga atatcagcca
    14881 gcattggggc aagggctggg ttgtccttag cgaagtttgc gtacattggc tgtactttct
    14941 cgataaacgg agctttatct acctggtaga ctttcacgcc cattttatcc agttttgcaa
    15001 attcttgttt ctcttcctcg ctccacaagt ctttcatgta gctcattgat tctttagctg
    15061 cttttaatat aactgcctga ttttccgggc taagtttatc aaacgcagca ttgctcatca
    15121 gaaccacatc gggaaccata gtgtgttcgt caaggctgaa ggattttgcc acttcaccgt
    15181 gacgcatggt ggtcagcgcc atcacgctgt tttctgccat atcgaccaca ccctgctgta
    15241 aggctgtata caactcgccg taatccagtg gcgtcgggtt accgcccatg actttgatca
    15301 tttcaactgc gccagggctt ggctggacac ggactttcat gcctttcaga tcgtctggtt
    15361 tcagtactgg tttattgcca tagaaactgc gggcgcctcc atcataaaaa gtcagcccga
    15421 aataaccttt gcttttcgtt gagtcgagga tttttctccc gatatcgccc tgcatgacct
    15481 gataataatg atcacgatcg cggaacaaat aaggcaggct aaataattta taggaatttt
    15541 caaatgcttc catttctgcc gcattggttt ttaccagtgg aatagcgcca gaacgaatca
    15601 gctccattgt ttctcgttga gtacccaatg ttccatttgg ataaatacga atagtaatat
    15661 caccgttaga gtactctttg ttctttttcg caaagaactc catcgcttta tgaacaggat
    15721 gagacttatc ctgattatga ctcagtttta atgtcactgc ccaggactgg cttgcggata
    15781 acataagaac agcaccgata atgccagcgg atacttttat tttcatagat gttccttttc
    15841 taataaatat ggcggcaggt gccgccagtg aatgcgcatc agtagcccag tgcttttggc
    15901 aatgccatac tgatttgcgg aatataggtc accattgcca gcaccaatac catcacggca
    15961 tagaatggca gcataggttt gataatttta ttaatcggta atttagatac actacaaccg
    16021 acaaataaca cactgcctac aggcggagta caaataccga tacacatgtt cagtaccatg
    16081 acaataccaa agtgaatcgg gtccatgccc agttcggtga caacaggtaa aaatatcggc
    16141 gtaaaaatca gtaccgccgg tgtcatatcc ataaaagtgc cgataatcaa caggataata
    16201 ttgataataa gcaggattat tattgggttg tcagaaatag tgttaagcaa atcgagaatc
    16261 aggaagggaa cgtcagcgtt tgacattgcc catga
//
LOCUS       DADMKF010000088        16182 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.090, whole genome shotgun sequence.
ACCESSION   DADMKF010000088 DADMKF010000000
VERSION     DADMKF010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16182
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.090"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(136..1164)
                     /locus_tag="JNC24_002600"
     CDS             complement(136..1164)
                     /locus_tag="JNC24_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mal regulon transcriptional regulator MalI"
                     /protein_id="HAY9159314.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGTTLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            1339..2931
                     /locus_tag="JNC24_002601"
     CDS             1339..2931
                     /locus_tag="JNC24_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HAY9159315.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESATRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVKDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            2941..4113
                     /gene="malY"
                     /locus_tag="JNC24_002602"
     CDS             2941..4113
                     /gene="malY"
                     /locus_tag="JNC24_002602"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HAY9159316.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDTQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCSPQNPTGKVWTCDELEIMADLCERHDVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYISDKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            4217..5218
                     /gene="add"
                     /locus_tag="JNC24_002603"
     CDS             4217..5218
                     /gene="add"
                     /locus_tag="JNC24_002603"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HAY9159317.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNGLHYVELRFSPG
                     YMAMAHQLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(5254..6294)
                     /locus_tag="JNC24_002604"
     CDS             complement(5254..6294)
                     /locus_tag="JNC24_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAY9159318.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSCVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            6538..6663
                     /gene="blr"
                     /locus_tag="JNC24_002605"
     CDS             6538..6663
                     /gene="blr"
                     /locus_tag="JNC24_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HAY9159319.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
     gene            6928..7143
                     /gene="ydgT"
                     /locus_tag="JNC24_002606"
     CDS             6928..7143
                     /gene="ydgT"
                     /locus_tag="JNC24_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HAY9159320.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            7229..7669
                     /locus_tag="JNC24_002607"
     CDS             7229..7669
                     /locus_tag="JNC24_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HAY9159321.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEDGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            7746..8327
                     /gene="rsxA"
                     /locus_tag="JNC24_002608"
     CDS             7746..8327
                     /gene="rsxA"
                     /locus_tag="JNC24_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HAY9159322.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLASICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFNGLVKL"
     gene            8327..8905
                     /gene="rsxB"
                     /locus_tag="JNC24_002609"
     CDS             8327..8905
                     /gene="rsxB"
                     /locus_tag="JNC24_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HAY9159323.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            8898..10832
                     /gene="rsxC"
                     /locus_tag="JNC24_002610"
     CDS             8898..10832
                     /gene="rsxC"
                     /locus_tag="JNC24_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HAY9159324.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNGTPLRQVPLTQRFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWADYRTRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPADLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKAARLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAAREARKAQARAKQAELQQTNDAATVADPRKTAVEAAIARA
                     KARKLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQQQTNAEPEEQVDPRKAAVA
                     AAIARAQAKKAAQQKVVNED"
     gene            10833..11891
                     /gene="rsxD"
                     /locus_tag="JNC24_002611"
     CDS             10833..11891
                     /gene="rsxD"
                     /locus_tag="JNC24_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HAY9159325.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSTLLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGLWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLIFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            11895..12515
                     /gene="rsxG"
                     /locus_tag="JNC24_002612"
     CDS             11895..12515
                     /gene="rsxG"
                     /locus_tag="JNC24_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HAY9159326.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKA
                     LFDQVLPAERYNNTLAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWITHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            12519..13214
                     /locus_tag="JNC24_002613"
     CDS             12519..13214
                     /locus_tag="JNC24_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HAY9159327.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGMGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRTEAA
                     AERALPNGETGNV"
     gene            13214..13849
                     /gene="nth"
                     /locus_tag="JNC24_002614"
     CDS             13214..13849
                     /gene="nth"
                     /locus_tag="JNC24_002614"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HAY9159328.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     VSVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            14460..15962
                     /gene="dtpA"
                     /locus_tag="JNC24_002615"
     CDS             14460..15962
                     /gene="dtpA"
                     /locus_tag="JNC24_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HAY9159329.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPINY
                     RNLLLTIIGVVALIAIATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAIIAVLMLLTAPKLHRMTQDDAADKAAKAAVA"
     gene            16067..>16182
                     /locus_tag="JNC24_002616"
     CDS             16067..>16182
                     /locus_tag="JNC24_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HAY9159330.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLE"
BASE COUNT         3764 a         4116 c         4123 g         4179 t
ORIGIN      
        1 tctcaagttg tcagaattaa acacataaac ctcctgtgaa cttcagtaaa aataaaaggc
       61 accgcctgtg tgccacattt tttatcatta accttgagga aaggacacta ccaggtatag
      121 cagataaaga gaggattatt tcgctgcaat gagccgggcg ggaataataa gattgcgtga
      181 atgcgtctct tcatgggtga ttttttgcat catgcgatcc gcaagtgtgg taccaagttc
      241 ccgcgcaggc gtgctggccc aggtaacggg tatatcatca agtgtggttg gtgtcgcatc
      301 ggtaaatgcc gccagcgaaa cttgttgctc aaagtaacga tcgactccgc tttccccgct
      361 ttgccgccct gctttcagca aaccaaacca tgcccccatc gcaatagttt cgttatagca
      421 aaccacagca ctgatggtcg ggttatgacg taaaagcgcc gtgatagctt ccgcggcttg
      481 cttctggctg gatgtgcact ccagcaccca atcgctgtga aacggcaggc caaattttag
      541 tagagttgca caatagcctc ccacccgttc tgcccgggtt aatgaggaac tttgccctcc
      601 cagccaggcg atccgctgat gcccattgcg aatgagatgc tccgtcaaca actgtgcagc
      661 ctgcatgttg tccgggcgaa ccgtatcaac atcatcaaga taactggcac gggaagcgaa
      721 aatcaccggg atagcttttt cttctgccat ccgtcgcagg tcatcgctgc ttcctgcagc
      781 cccggcaatt accacaccat cgacaccctg attcagtaac agtgaaaacc gctgtgccag
      841 ctgctcgccg tctttaccgc cgtgaagcaa aaaaaccatc cgtccctgcg cttccagagc
      901 ttccgtcaat ccggccgtca attcggcgta aaacggcgca gataaatcac ggacgatcaa
      961 accaatgacg ccgctttgcc cgccgcgcag cgccgacgcc tggcgattgc gcacaaatcc
     1021 cagctcttca atggcggcgt taacgcgttc tcctgtggcg gtagagattc gccctttgcc
     1081 actaagcacc agcgaaacgg tacttaccga cacgcccgca gccagcgcaa catcatgaat
     1141 ggttattttt ttggcggtag ccatgcagat gacctactcc ctgattatgt gacagataaa
     1201 acgttttacc ttttatttat cttatacccg ctattatcgt tgcgtaatgt gatttatgct
     1261 tcactaaaat ttgataaaac gttttatctt ctcgcgcaat ttactgaatc cagattgttc
     1321 tctacgagga gtcgttttat gacggcgaaa acagcaccga aagtcacgct gtgggagttc
     1381 ttccagcagt taggcaaaac cttcatgtta cccgtggcat tattgtcgtt ctgcggcatt
     1441 atgctcggca ttggtagttc tcttagtagc cacgatgtca tcaccctgat cccggtcctg
     1501 ggcaaccccg tgttgcaggc tatctttacc tggatgagta agattggctc gtttgctttt
     1561 agtttcctgc cagtcatgtt ctgtatcgcc atcccgctgg gtctggcacg cgaaaacaaa
     1621 ggcgtagcgg cattcgctgg cttcgtcggt tatgcggtaa tgaacctcgc ggtaaacttc
     1681 tggttgacca ataaaggcat tctgccaacc acggacgccg cggttctgaa agccaataac
     1741 atccagagca ttcttgggat ccagtcgatc gacaccggga tcctcggtgc ggtgatcgcc
     1801 ggtattatcg tctggatgct gcatgagcga ttccataata tccgcctgcc ggatgcgctt
     1861 gcattcttcg gcggtacgcg cttcgtacca atcatctcct cgctggtgat gggccttgtc
     1921 ggcctggtga ttccattagt ctggccgatt ttcgcgatgg gtattagcgg cttgggccat
     1981 atgataaaca gcgcgggtga tttcggaccg atgctgtttg gtaccggtga acgtctgctg
     2041 ttgccgtttg gtctgcatca cattctggtg gcattaattc gctttaccga cgcaggcggc
     2101 acgcaggaag tctgcggtca aaccgtcagc ggcgcattga ccatcttcca ggcgcaattg
     2161 agttgcccga ccactcacgg tttttctgaa agcgccacgc gtttcctttc gcaaggcaaa
     2221 atgcctgcgt ttctcggcgg tctgccaggt gcagcgttag cgatgtatca ctgcgcgcgc
     2281 ccggaaaatc gccataaaat taaagggctg ctgatttctg gcctgatcgc ctgtgtcgtt
     2341 ggcggcacga ccgaaccgct ggaattcctg ttcctgttcg tggcgccagt tctgtatgtc
     2401 atccacgcgc tgttaaccgg cctcggcttc actgtcatgt ctgtgctcgg cgtcaccatc
     2461 ggtaataccg acggcaatat catcgacttc gtggtgttcg gtattttgca tggtctgtca
     2521 accaagtggt acatggtgcc agtggtggcg gcaatctggt ttgtcgttta ctacgtcatc
     2581 ttccgtttcg ctatcacccg cttcaatctg aaaaccccgg ggcgcgatag cgaagtggcc
     2641 agttcaatcg aaaaagccgt tgccggtgcg ccgggtaaat caggttacaa cgttccggca
     2701 atcctcgaag ccttaggcgg tgccgacaat attgttagcc tcgataactg cattacccgt
     2761 ctgcgtttgt ctgtgaaaga tatgtcgctt gttaatgtgc aggcactgaa ggacaatcgg
     2821 gcaattggcg tagtacaact taatcaacat aacctgcagg ttgttatcgg gccacaagtt
     2881 cagtcagtaa aagatgaaat ggccggtctg atgcatactg tccaggcata aggataagat
     2941 atgttcgatt tttcaaaggt cgtggatcgt catggcacat ggtgtacaca gtgggattat
     3001 gtcgctgacc gtttcggcac tgctgacctg ttaccgttca cgatttcaga catggatttt
     3061 gccactgccc cctgcattat cgaggcgctg aatcagcgcc tgatgcacgg cgtatttggc
     3121 tacagccgct ggaaaaacga tgagtttctc gcggctattg cccactggtt ttccacccag
     3181 cattacaccg caattgatac gcagacggtg gtgtatggcc cttctgtcat ctatatggtt
     3241 tcagaactga ttcgtcagtg gtctgaaaca ggtgaaggcg tggtgatcca cacgcccgcc
     3301 tatgacgcat tttacaaggc cattgaaggt aaccagcgca cagtaatgcc cgttgcttta
     3361 gagaagcagg ctgacggctg gttttgcgat atgggcaagc tggaagccgt tttggcgaaa
     3421 ccagaatgta aaattatgct cctgtgtagc ccacagaatc ctaccgggaa agtgtggacg
     3481 tgcgatgagc tggagatcat ggctgacctg tgcgagcgtc atgatgtgcg ggttatttcc
     3541 gatgaaatcc atatggatat ggtctggggc gagcagccgc atattccctg gagtaatgtg
     3601 gcgcggggag actgggcgtt gctgacgtcg ggatcgaaaa gtttcaatat tcccgcgctg
     3661 accggtgctt acgggattat agaaaatagc agcagccgtg atgcctattt atcggcactg
     3721 aaaggacgtg atgggctttc ttccccttcg gtactggcgt taactgccca tatcgccgcc
     3781 tatcagcaag gtgcaccgtg gctggatgcc ttacgcgtct atctgaaaga taacctgacg
     3841 tatatctcag ataaaatgaa cgccgcgttt cctgaactca actggcagat cccgcaatcc
     3901 acttatctgg cctggcttga tctacgtccg ttgaatattg acgacaacgc gttgcaaaaa
     3961 gcgcttatcg agcaagaaaa agtcgcgatc atgccggggt atacctatgg tgaagaaggt
     4021 cgtggttttg tccgtctcaa tgccggctgc ccacgttcga aactggaaaa aggtgtggct
     4081 ggattaataa acgccatccg cgctgttcgc taaccccaat tgcgcaacgt aaaaaatcgt
     4141 tgcgcaatcg tggattttta ccctgctttg tttttataat ggtgcgcact tttatatcca
     4201 gaaaaagagt gcgaccatga ttgataccac cctgccatta actgatatcc atcgccacct
     4261 tgatggcaac attcgtcccc agaccattct tgaacttggc cgccagtata atatctcgct
     4321 tcctgcacaa tccctggaaa cactgattcc ccacgttcag gtcattgcca acgaacccga
     4381 tctggtgagc tttctgacca aacttgactg gggcgttaaa gttctcgcct ctcttgatgc
     4441 ctgccgccgc gtggcatttg aaaacattga agatgcagcc cgtaacggcc tgcactatgt
     4501 cgagctgcgt ttttcaccag gctacatggc aatggcacat cagctgcctg tagcgggtgt
     4561 tgtcgaagcg gtgatcgatg gcgtacgtga aggttgccgc acctttggtg tgcaggcgaa
     4621 gcttatcggc attatgagcc gaaccttcgg cgaagccgcc tgtcagcaag agctggaggc
     4681 ctttttagcc caccgtgacc agattaccgc acttgattta gctggtgatg aacttggatt
     4741 cccgggaagt ctgttccttt ctcacttcaa ccgcgcgcgt gatgcgggct ggcatattac
     4801 cgtccatgca ggcgaagctg ccgggccgga aagcatctgg caggcgattc gtgaactggg
     4861 tgcggagcgt attggacatg gcgtaaaagc cattgaagat cgggcgctga tggattttct
     4921 cgccgagcaa caaattggta ttgaatcctg tctgacctcc aatattcaga ccagcaccgt
     4981 agcagagctg gctgcacatc cgctgaaaac gttccttgag catggcattc gtgccagcat
     5041 taacactgac gatcccggcg tacagggagt ggatatcatt cacgaatata ccgttgccgc
     5101 gccagctgct gggttatccc gtgagcaaat ccgccaggca cagattaatg gtctggaaat
     5161 ggctttcctc agcgcagagg aaaaacgcgc actgcgagaa aaagtcgccg cgaagtaaca
     5221 aaaatggatg gtgcaatttg caccatccat ttttcacgca aggcacaacg tcgcgcgatg
     5281 tttggcggat tcgatgccca gctcaatcaa ctccattacc tggattgcct ggcttgccgg
     5341 aaccggattt tcaccatcgc catttaacgc atcacgaatc gccgcatagt aagccggata
     5401 attcccaggc accgtcaaca gcgtttcttc gacacgttcc tcaccttcca cgcgggtaag
     5461 tacgccatca cgcatatcgt agccccagtc ttcctgcggc agacgctcgc catttttcag
     5521 acgttcttcc tgtggatcga ggccatattt cacacaactg cctcgggatc catgcacgat
     5581 ataccgtgct gactcagcgg ctgccagcat ggtaccgtgt aaaatgactc gccgctgtgg
     5641 ataggacaag atggcgtgga aataatcggt cgactgcgct ccgggccgta actgtgccaa
     5701 atctaccgtc atgctgaccg gtaaaccaaa tagcgtaatg gcctgatcaa gaagatgcgg
     5761 tgctaaatcg taccagatac cgctgcctgg gccgccctgt tcacgccaac gatcgcgcac
     5821 ctgcggacgg aagcggtcaa aatgagactc aaagtaagca acttcaccca gcacgccttc
     5881 cgcgagcaaa ccttttagcg tcaagaaatc gctatcccaa cgacggttat ggaatacaga
     5941 cagcacacgc cccaggcttt ttgccagcgc atccagctct cgcgcttgtg acagtgtcac
     6001 ggtaaagggt ttatcaacga ccacatgttt acccgcctca agcgccgctt tggctaacgg
     6061 gaaatgggta tcgttgggtg taggaatgac aatcaggtct atgttgggat cgttaaacag
     6121 atgcttcggc tcagagacaa ccgtaaccgt tggccagtcg gcttttactt ttgtttcatc
     6181 actgctggag attaccgcca gttccagccc gggcgtgccc gcaatcaggg gcgcatggaa
     6241 ggttttgctc gcataaccat acccaatcaa cccaacacgg atgttgtcgc tcatgtcatt
     6301 gcctctcatt gaagtatgat ggctatttga cactatcctt tacccacgct caacagttta
     6361 ataacctgcc agcaataagg gatgttgttt aacttaagtc aaaaaaaata gcgaattttc
     6421 caacgacaaa agctaaatat cgcaaaaacc tcagtaaaaa tcttgctgga gctattattg
     6481 ctaagtaaca tttaccccct gaagttaatg gatcaatcaa gagagatgtg ggctgtaatg
     6541 aatcgtctta ttgaattaac aggttggatc gttcttgtcg tttcagtcat tcttcttggc
     6601 gtggcgagtc acattgacaa ctatcagcca cctgaacaga gtgcttcggt acaacacaag
     6661 taagctctgc acttgtggag cgacatgctg cccgtccggg tgcatgtttt cacttgtcgg
     6721 atattaaacc aggaatttat tatcttgttc gatgttgttg gtgattgtca gggatagtaa
     6781 agcgcgttac tcttttcaga gcagtttttc atccctgaat atttgccatc agcgcattct
     6841 tattattgta ataacacaag cgttcacttt tggtgaaatt ttatcatcac tctacccctt
     6901 tggtattaat tattttatgg accttttatg actgttcagg actacttatt aaaatttcgc
     6961 aaaatcagtt cactcgaaag tctggaaaaa ctctacgacc atcttaatta caccctgacg
     7021 gacgatcagg aactgatcaa tatgtatcgt gctgccgatc accgtcgcgc agagctggtt
     7081 tctggcgggc gtttgtttga cctcggccag gtaccgaagt ccgtctggca ctatgtccaa
     7141 taaagaaagt agcgatcggt tataaaagcc ttataataac gcctctaaaa ttagatttgc
     7201 ctgtttgcgt cgctattctg gagaacctat gaccaccaca acgccacaac gtattggagg
     7261 ctggttgctt ggcccgttgg cctggctttt agtcgctctg ttgagtacga cgctggcgtt
     7321 gttgctgtat accgctgcgt tatcttctcc gcaaacattt caaacgctcg gcggacaagc
     7381 actgacgaca caaatcttat ggggcgtttc ttttattacc gctatcgcca tgtggtatta
     7441 cacactgtgg ttgaccatcg ctttctttaa acgtcggcgt tgtgttccta aacactatat
     7501 tatctggctg cttatttccg tattgctggc agtaaaagca ttcgcttttt cacccgtgga
     7561 agatggcata gccgttcgcc agttgctgtt tactttgttg gcaaccgcat tgatcgtccc
     7621 ctatttcaaa cgctcgtcgc gggtaaaagc gacgtttgtg aatccgtaat aaccttacag
     7681 ttaacctgtt gtcgcctgct ctggattaac ggataatagg cggctttttt atttcaggcc
     7741 gaaaaatgac tgactacctg ttactgtttg tcggaactgt actggtcaat aactttgtac
     7801 tggtcaagtt tctcggtctc tgtccgttta tgggggtttc caaaaaactg gaaaccgcga
     7861 tgggcatggg gctggcaaca acgtttgtga tgacgctggc gtctatttgc gcctggctta
     7921 tcgatacgtg gattttgatc ccacttaatc tgatttacct gcgcaccctg gcatttattc
     7981 tggtgattgc tgtggtcgtg cagttcaccg agatggtggt gcgcaaaacc agcccggtgc
     8041 tttaccgtct gctggggatt tttttgccgc ttatcaccac caactgtgca gtgctcggcg
     8101 tggcgttgct gaatatcaat ctcgggcaca atttcttgca gtcggcgctg tacggttttt
     8161 ccgccgctgt cggtttctcg ctggtgatgg tgctcttcgc cgccatccgc gaacgccttg
     8221 ctgtggctga tgtcccggca ccttttcgcg gtaatgccat tgcgttaatt accgcaggtc
     8281 ttatgtctct ggcctttatg ggctttaatg gtttggtgaa gttgtaatga atgctatctg
     8341 gattgccgtt gccgccgtga gcctgctggg cctggcgttt ggcgccattc tgggttatgc
     8401 ctcccgccgt tttgcggtgg aagacgatcc ggtcgttgag aaaattgacg aaatcttacc
     8461 gcagagccag tgtggtcagt gcggttatcc cggctgtcgc ccctacgcgg aagccatcag
     8521 ctgtaacggt gaaaaaatca accgttgcgc cccaggtggc gaagctgtga tgctaaaaat
     8581 tgccgagttg cttaatgtcg agccgcagcc gctggatggc gaagcgcaag agctaacgcc
     8641 tgcgcggatg gtggcggtta ttgatgaaaa taactgtatt ggctgcacta aatgtattca
     8701 ggcttgtccg gtagacgcca tcgttggcgc tacccgtgcc atgcatacgg taatgagtga
     8761 tctctgtacg ggctgcaatt tatgtgttga tccgtgcccg acgcactgca tctcgttgca
     8821 accggtcgca gaaacacctg actcctggaa atgggatctg aacaccattc ccgtgcgtat
     8881 cattcccgtg gaacaccatg cttaagttat tctctgcatt cagaaaaaat aaaatctggg
     8941 atttcaacgg cggcatccat ccaccggaga tgaaaaccca gtccaacggt acacccctgc
     9001 gccaggtacc actgacgcag cgttttgtta ttccactgaa acagcatatt ggcgctgaag
     9061 gtgagttgtg cgttagcgtc ggcgataaag tattgcgcgg ccagccgctt acccgtggtc
     9121 gcggcaaaat gctgcctgtt cacgcgccca cctcgggtac cgttacggct attgcgcccc
     9181 actctacggc tcatccttca gctttagctg aattaagcgt gattattgat gccgatggtg
     9241 aagactgctg gatcccgcgc gacggctggg ccgattatcg cactcgcagt cgcgaagagt
     9301 taatcgagcg catacatcag tttggtgttg ccgggctggg cggtgcagga ttcccgacag
     9361 gcgttaaatt gcagggtggc ggagataaga ttgaaacgtt gattatcaac gcggctgagt
     9421 gcgagccgta cattaccgcc gatgaccgtt tgatgcagga ttgcgcggct caggtcgtag
     9481 agggtattcg cattcttgcg catattctgc agccacgcga aattcttatc ggcattgaag
     9541 ataacaaacc gcaggcgatt tccatgctgc gcgcggtgct ggcggactct aacgatattt
     9601 ctctgcgggt gattccaacc aaatatcctt ctggcggtgc taaacaatta acctacattc
     9661 tgaccgggaa gcaggttcca catggcgggc gttcatccga tatcggcgta ttaatgcaaa
     9721 acgtcggcac tgcttatgca gtgaaacgtg ccgttattga tggcgagccg attaccgagc
     9781 gtgttgtaac cctgactggc gaagcaatcg ctcgcccggg caacgtctgg gcacggctgg
     9841 ggacgccagt gcgtcattta ttgaatgatg ccggattctg cccctctgcc gatcaaatgg
     9901 tgattatggg tggcccgcta atgggcttta ccttgccatg gctggatgtc ccggtcgtaa
     9961 agattaccaa ctgtctgttg gctccctctg ccaatgaact tggcgaacca caggaagaac
    10021 aaagctgcat ccggtgtagc gcctgtgctg acgcctgccc tgcggatctt ttgccgcaac
    10081 agttgtactg gttcagcaaa ggtcagcaac acgataaagc taccacgcat aacattgctg
    10141 attgcattga atgtggggct tgcgcgtggg tttgcccgag caatattccc ctggtgcaat
    10201 atttccgtca ggaaaaagct gaaattgcgg ctattcgtca ggaagaaaag cgcgccgcag
    10261 aagccaaagc gcgtttcgaa gcgcgccagg ctcgtctgga gcgcgaaaaa gcggctcgcc
    10321 ttgaacgaca taagagcgca gccgttcaac ctgcagccaa agataaagat gcgattgctg
    10381 ccgctctggc gcgggtgaaa gagaaacagg cccaggctac acagcctatt gtgattaaag
    10441 cgggcgaacg cccggataac agtgcaatta ttgcagcacg ggaagcccgt aaagcgcaag
    10501 ccagagcgaa acaggcagaa ctgcagcaaa ctaacgacgc agcaaccgtt gctgatccac
    10561 gtaaaactgc cgttgaagca gctatcgccc gcgccaaagc gcgcaagctg gaacagcaac
    10621 aggctaatgc ggaaccagaa gaacaggtcg atccgcgcaa agccgccgtc gaagccgcta
    10681 ttgcccgtgc caaagcgcgc aagctggaac agcaacagac taacgcagaa ccagaagaac
    10741 aggttgatcc gcgcaaagcg gcagttgccg cggctattgc ccgcgctcag gccaaaaaag
    10801 ccgcccagca gaaggttgta aacgaggact aaatggtatt cagaatagct agctcccctt
    10861 atacccataa ccagcgccag acatcgcgca ttatgctgtt ggtgttgctc gcagccgtgc
    10921 caggaatcgc agcgcaactg tggttttttg gttggggtac tctcgttcag atcctgttgg
    10981 catcggttag tactctgtta gccgaagctc tcgtactcaa actacgcaag cagtcggtag
    11041 ccgcaacgtt gaaagataac tcagcattgc tgacaggctt attgctggcg gtaagtattc
    11101 cccccctcgc gccatggtgg atggtcgtgc tgggtacggt gtttgcggtg atcatcgcta
    11161 aacagttgta tggcggtctg gggcaaaacc cgtttaatcc ggcaatgatt ggttatgtgg
    11221 tcttactgat ctccttcccg gtgcagatga ccagctggtt accgccacat gaaattgcgg
    11281 tcaacatccc tggttttatc gacgccatac aggttatttt cagcgggcat accgccagtg
    11341 gtggtgatat gaacacacta cgcttaggta ttgatggcat tagtcaggcg acaccgctgg
    11401 atacatttaa aacctctgtc cgtgccggtc attcggttga acagattatg caatatccga
    11461 tctacagcgg tattctggcg ggcgctggtt ggcaatgggt aaatctcgcc tggctggctg
    11521 gcggcctgtg gttgctatgg cagaaagcga ttcgctggca tattcccctc agcttcttag
    11581 taacgctggc gttatgcgca acgttgggct ggttgttctc accagaaaca ctggcagcac
    11641 cgcaaattca tctgctgtct ggtgcgacca tgctcggcgc attctttatt ttgactgacc
    11701 cggttaccgc ttctacgacc aatcgtggtc gtcttatttt cggcgcgctt gcgggcttat
    11761 tagtctggtt gatccgcagt ttcggcggct atcctgacgg cgtggctttt gccgtcctgc
    11821 tggcgaacat cacggttcct ctgatcgatt actacacgcg tccgcgcgtc tacggccatc
    11881 gcaaagggta aaccatgctg aaaactatcc gaaaacacgg cattacgtta gcgctatttg
    11941 cagcgggttc aacagggtta actgcggcca tcaaccagat gaccaaaacg acgattgctg
    12001 aacaggccag tctgcaacaa aaggcattat ttgatcaggt gctgccagcc gaacgctata
    12061 acaatacgct ggcacagagt tgctatctgg taactgcgcc agagttaggt aaaggtgagc
    12121 atcgggttta catcgccaaa caggatgaca aaccggtagc cgccgttctg gaagcaaccg
    12181 cgccagatgg ctattccggt gcgattcagc tgctggtggg agccgatttt aacggcacgg
    12241 tacttggcac gcgcgtgaca gagcaccacg aaacgccagg gcttggcgat aaaatcgaac
    12301 tgcgcctttc tgactggatc acccattttg cgggtaaaaa aatcagtggt gcggatgatg
    12361 cgcactgggc ggtgaagaaa gatggtggtg atttcgacca gttcaccggc gcgacgatta
    12421 ctccccgcgc ggtggttaat gcggtaaaac gcgccggatt gtacgctcag acgttaccgg
    12481 cacaactttc tcaacttcct gcctgtggag aataaagcgt gagcgaaatt aaagacgtta
    12541 ttgttcaggg gttgtggaaa aacaactctg cgctggtcca gttgctcggc ctttgtcctc
    12601 tgttggcggt cacgtccact gccactaacg ctctgggttt aggacttgcg actacgctgg
    12661 tactgacgct gaccaacctg accatttcga cgctgcgtca ctggacgcca gccgagatcc
    12721 gcattcccat ttacgtgatg atcatcgcct cggtggtcag cgctgtacag atgctgatca
    12781 acgcctacgc ctttggcctg tatcaatcat tagggatttt tattccgctg attgtcacta
    12841 actgtatcgt tgtgggccgc gctgaagcct tcgccgccaa aaaaggtccg gcgctttcgg
    12901 cactggacgg cttttcaatt ggtatgggcg caacctgcgc catgttcgtg ctgggttcac
    12961 tacgcgaaat tatcggcaat ggcacattgt ttgacggtgc agatgcgctg ttaggtagct
    13021 gggcaaaagt attacgcgtg gagattttcc acaccgactc ccctttcctg ctggcgatgc
    13081 tgccaccagg tgcatttatt ggcctgggac tgatgctggc aggaaaatac ctgattgatg
    13141 aaagaatgaa aaagcgccgt actgaagcag ctgccgaacg tgcattgcca aacggtgaaa
    13201 cagggaatgt ctgatgaaca aagcaaaacg cctggagatc ctcactcgcc tgcgtgagaa
    13261 caatcctcat cccaccaccg agcttaattt cagttcgcct tttgaattgc tgattgccgt
    13321 actgctttcc gctcaggcga ccgatgtcag tgttaataag gcgacggcga aactttaccc
    13381 ggtggcgaat acgcctgcag cgatgcttga actgggcgtt gaaggggtta aaacctatat
    13441 caaaacgatt gggctttata acagcaaagc agaaaatatc atcaaaacct gccgtatctt
    13501 actggagcag cataatggcg aggttccgga agatcgtgct gcgcttgaag ccctgcccgg
    13561 cgtaggtcgt aaaacagcca acgtcgtatt aaacactgca ttcggctggc cgactattgc
    13621 tgtcgacacg cacattttcc gcgtttgtaa tcgtactcaa tttgcgccgg ggaaaaacgt
    13681 cgaacaggta gaagaaaagc tactgaaagt ggttccggca gagtttaaag tcgactgcca
    13741 ccattggttg atcctgcacg ggcgttatac ctgcattgcc cgcaagcccc gctgtggctc
    13801 ttgtattatt gaagatcttt gtgaatacaa agagaaagtt gacatctgaa gaaaaggggt
    13861 aacaccgatt accccattga taacctttct ttatcctctt ttaaaacatc tttttaacgt
    13921 gaatgatgcc attgcttagc gttatcatca ggtaatccgt ttgcggataa ccaaaaatgc
    13981 aggttaattg tttttttaat agcgaaattt tctattcatt cgtgatcaag atcacgatgc
    14041 tgtcgcaatg taaaaacatt gcaagaatca ggttaaaata atttaaattt acacacgcaa
    14101 caaatattga cctacaaaac attacactgg ctatttttca gaaactggac tatctcacta
    14161 gtaaaaacgc taaatagcag aacatatcgc cgttaagcca tcacccccct ctcagtgcag
    14221 tgaaaaaatc tgccattacg ttttttgaaa aatttaacgc tggataacat ttcccggaat
    14281 ggttgaattc cccgcctcag ttatatgtaa cagattatta caaaggactt gtctgaaagt
    14341 gcaagatagt gaacattacc tgccgtttcc cctcccacta taacaattgc gcgtatgttt
    14401 tttagacata acgcgagaaa gcacccccgt taatatggga tgtaaaaaaa gaggtaaaag
    14461 tgtccactgc aaaccaaaaa ccaactgaaa gcgtcagttt gaacgctttc aaacaaccga
    14521 aggcgttcta tctcatcttc tcgattgagt tatgggaacg ttttggttat tacggcctac
    14581 aaggaattat ggctgtttac ctggttaaac aactgggtat gtctgaagcg gattcaatca
    14641 cccttttctc ttcctttagt gccctggttt atggtctggt cgctatcggc ggctggttag
    14701 gtgacaaggt actgggtact aaacgcgtaa ttatgctcgg tgctattgtg ctggcgattg
    14761 gttatgctct ggttgcctgg tctggtcacg acgccggtat cgtttatatg ggtatggcgg
    14821 ctattgcggt cggtaacggc ctgtttaaag ctaacccatc ttctctgctt tctacatgct
    14881 atgagaaaaa cgacccgcgt ctggacggtg cattcaccat gtactacatg tccgtcaaca
    14941 tcggctcttt cttctctatg attgctacgc cgtggctggc cgcgaaatac ggctggagtg
    15001 ttgcgtttgc gttgagcgtt gtaggcctgc tgatcactat cgttaacttc gccttctgcc
    15061 aacgctgggt taaacagtac ggttcaaaac cagacttcga gcctatcaac taccgtaacc
    15121 tgctgctgac cattattggt gttgtggcac tgatcgctat cgccacctgg ctgctgcaca
    15181 atcaggaagt tgcgcgtatg gcgctgggcg ttgttgcctt cggtatcgtg gttatcttcg
    15241 gtaaagaagc cttcgcgatg aaaggtgctg cgcgtcgtaa aatgatcgtt gccttcatcc
    15301 tgatgctcga agccattatc ttcttcgtgc tgtacagcca gatgccaacg tcactgaact
    15361 tctttgcgat tcgtaacgtt gagcactcca ttctgggtct ggccgtagaa cctgagcagt
    15421 atcaggcact gaacccgttc tggatcatca tcggtagtcc gattctggcc gctatctata
    15481 acaagatggg cgataccctg ccgatgccaa ccaagtttgc aatcggcatg gtgatgtgtt
    15541 ctggtgcgtt cctgattctg ccgctgggtg cgaaattcgc gtctgacgct ggtatcgtgt
    15601 ctgtaagctg gctggtcgca agctatggcc tgcagagcat cggggaactg atgatctctg
    15661 gtctgggtct ggcaatggtt gcacaactcg ttccgcagcg tctgatgggc ttcattatgg
    15721 gtagctggtt cctgaccact gccggtgcaa acctgattgg tggttatgtt gcgggtatga
    15781 tggctgtgcc ggataacgtt accgacccgc tgatgtcact ggaagtttat ggtcgcgtat
    15841 tcttgcagat tggtgtcgct actgccatta ttgcagtact gatgctgctg accgcgccta
    15901 aactgcaccg tatgacgcag gatgacgctg cagacaaagc ggcgaaagct gctgtagcgt
    15961 aaaattcagg gaaactcttt tacaagccgc taacttttcg ttagcggctt ttttttgttc
    16021 agcaacgcac taccatactt taaaccacag acaaaaggag ttaccgatga aattgttcta
    16081 caaaccgggt gcctgctctc tcgcttccca tatcaccctg cgtgagagcg gaaaggattt
    16141 taccctcgtc agtgtggatt taatgaaaaa acgtctcgaa aa
//
LOCUS       DADMKF010000089        16017 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.091, whole genome shotgun sequence.
ACCESSION   DADMKF010000089 DADMKF010000000
VERSION     DADMKF010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16017
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.091"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            26..199
                     /locus_tag="JNC24_002617"
                     /pseudo
     CDS             26..199
                     /locus_tag="JNC24_002617"
                     /inference="COORDINATES: protein motif:HMM:NF020822.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
     gene            593..1453
                     /locus_tag="JNC24_002618"
                     /pseudo
     CDS             593..1453
                     /locus_tag="JNC24_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001434157.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 38"
     gene            complement(1541..2512)
                     /gene="lpxM"
                     /locus_tag="JNC24_002619"
                     /gene_synonym="msbB"
     CDS             complement(1541..2512)
                     /gene="lpxM"
                     /locus_tag="JNC24_002619"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416369.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HAY9159331.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVDWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IARRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(2632..3954)
                     /gene="mepM"
                     /locus_tag="JNC24_002620"
     CDS             complement(2632..3954)
                     /gene="mepM"
                     /locus_tag="JNC24_002620"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HAY9159332.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAAHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            complement(3970..4902)
                     /gene="znuA"
                     /locus_tag="JNC24_002621"
     CDS             complement(3970..4902)
                     /gene="znuA"
                     /locus_tag="JNC24_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HAY9159333.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPGAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            4981..5736
                     /gene="znuC"
                     /locus_tag="JNC24_002622"
     CDS             4981..5736
                     /gene="znuC"
                     /locus_tag="JNC24_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HAY9159334.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNRPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            5733..6518
                     /gene="znuB"
                     /locus_tag="JNC24_002623"
     CDS             5733..6518
                     /gene="znuB"
                     /locus_tag="JNC24_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HAY9159335.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQRVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(6665..7675)
                     /gene="ruvB"
                     /locus_tag="JNC24_002624"
     CDS             complement(6665..7675)
                     /gene="ruvB"
                     /locus_tag="JNC24_002624"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017441950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HAY9159336.1"
                     /translation="MIEADRLISAGTTLPEDGADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(7684..8295)
                     /gene="ruvA"
                     /locus_tag="JNC24_002625"
     CDS             complement(7684..8295)
                     /gene="ruvA"
                     /locus_tag="JNC24_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HAY9159337.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARTDASSETLIREALRAAL"
     gene            complement(8434..8499)
                     /locus_tag="JNC24_002626"
     CDS             complement(8434..8499)
                     /locus_tag="JNC24_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9159338.1"
                     /translation="MYIFITHLFTEYVILKYLLPI"
     gene            8572..9174
                     /locus_tag="JNC24_002627"
     CDS             8572..9174
                     /locus_tag="JNC24_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HAY9159339.1"
                     /translation="MNINYPAEYEIGDIVFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSSNQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGELLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
     gene            complement(9176..9697)
                     /gene="ruvC"
                     /locus_tag="JNC24_002628"
     CDS             complement(9176..9697)
                     /gene="ruvC"
                     /locus_tag="JNC24_002628"
                     /EC_number="3.1.22.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HAY9159340.1"
                     /translation="MAIILGIDPGSRVIGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGIGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLARGRLR"
     gene            complement(9732..10472)
                     /locus_tag="JNC24_002629"
     CDS             complement(9732..10472)
                     /locus_tag="JNC24_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAY9159341.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAVDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            complement(10501..10953)
                     /gene="nudB"
                     /locus_tag="JNC24_002630"
     CDS             complement(10501..10953)
                     /gene="nudB"
                     /locus_tag="JNC24_002630"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HAY9159342.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQVVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            complement(11071..12843)
                     /gene="aspS"
                     /locus_tag="JNC24_002631"
     CDS             complement(11071..12843)
                     /gene="aspS"
                     /locus_tag="JNC24_002631"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HAY9159343.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADAMGALRLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTVLAELSIQVVKKAENN"
     gene            13153..13719
                     /locus_tag="JNC24_002632"
     CDS             13153..13719
                     /locus_tag="JNC24_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAY9159344.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVDAPSPAKVLPENWWQHPAALGATDSDIEIIKR
                     QWGAFYGTDLELQLRRRGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAAS
                     AEQHNNSINHIYPRIARVRSVEEILHAL"
     gene            14074..14322
                     /locus_tag="JNC24_002633"
     CDS             14074..14322
                     /locus_tag="JNC24_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI family protein"
                     /protein_id="HAY9159345.1"
                     /translation="MRIEICIAKEKMTKMPTGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
     gene            14923..>16017
                     /locus_tag="JNC24_002634"
     CDS             14923..>16017
                     /locus_tag="JNC24_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HAY9159346.1"
                     /translation="MLPTNNNHRLISNSFSTYSIDTSRAYENYLTHWTEWKNNRIQEE
                     QRDIAFQRLVSCLQNQETNLDLSELGLTTLPEIPPEIKSINISKNNLSLISPLPASLT
                     QLNVSYNRLIELPALPQGLKLLNASHNQLITLPTLPISLKELHVSNNQLCSLPVLPEL
                     LETLDVSCNGLAVLPPLPFSLQEISAIGNLLSELPPLPHNIHSIWAIDNMLTDIPYLP
                     ENLRNGYFDINQISHIPESILNLRNECSIDISDNPLSSHALQSLQRLTSSPDYHGPQI
                     YFSMSDGQQNTLHRPLADAVTAWFPENKQSDVSQIWHAFEHEEHANTFSAFLDRLSDT
                     VSARNTSGFREQVAAWLEKLSASAELRQQSF"
BASE COUNT         4063 a         4075 c         3671 g         4208 t
ORIGIN      
        1 attttttata actattacta ataatatgac atcaattctt cgcagtgagt ttgatgaaat
       61 atggaaagcg atgctgacct tgcaattcca tgacatcctt cccggctcat gtatttcaag
      121 agtttatcat gaaactgaaa aggaatatct taaactagaa gcaaaaaccg agaaaattat
      181 ttctgatgcg cagtctactt tattaagtaa aattgacact tcttcttata aagatccgca
      241 cattttgttt aataccacct gttttgctcg taacgaatgg atcaatataa acaacaactg
      301 gctcaaagca cgagtaaact cttatggtta tgctgtgatt gttccaaaga ataagatcgt
      361 aaatggtctg aaggcagaat ctcaaagtat tgaaaataat tacattaaac ttcttttttc
      421 agaaagcggt gatttaattt cgctatatga taaaagatat ggtaaagaat acattactga
      481 aaatatgcac agtgagatcc gtgcatacca cgaagatgca ggttttttcg cggcctggga
      541 ttttgcttca aattatcgtg atggggaatc atatgtttta ctcgctgaaa aaatgacaac
      601 tgtgatcagc gggcctaaaa ctacgatgac gctgatttat cactacaatt cctcttatct
      661 tcgtttagcc tttaccctga cacaggatag tccacgcgtt gatgtccaaa cgtttattga
      721 ttggcacgaa cctaatgttt ctctcaaagt aaaattcccg gtgtcagtac aaacatctct
      781 tgcacaatgt caaattcagt tcggtgtaat tgacagacca acgcatagcg atgattcgtt
      841 cgcttttgcc aaagatgaaa taccagcaca tcactgggtc gacctttctg atcgagaaac
      901 tggcatagct ttaatagcaa aaaacaaata tggctatcgt gtcaaagacc agacgcttga
      961 attaactttg ctgcgttcgc agcataaacc tggggaagtt gttaagacag acaattatga
     1021 taatttcctg attaataatt ttgcagatat tggtaaacaa aaattcatat tctccttatt
     1081 tcctcatcat ggagattacc gtgtgggtgg tgttattaat caagcctata taatgaatca
     1141 tcccttacag gtcgtccctg tcaaaccgca cccgggtgaa ttaccccctt cactatcatt
     1201 ttttgctatc gatactaaca gcgttatcat cgaaacaata aaattagctg aagacggcga
     1261 cggtataatc gtaagattat atgagagtca tggattagaa atttcagcca tgctttcgtg
     1321 agtatgtaat tatcactacg ttcaatatgt cgatcttcaa gaaaataagt tagatgacag
     1381 tttttattgc aatcaatatt gcaatcttga gttcaaacct tttgaaatta tcacgctacg
     1441 attaactcaa tagcgtacta acctaaaaac tcaaaatagt gggaacactc attgagattt
     1501 tttatcgagt acagcctctc aataatttga gaggcttttt ttatttgata ggataaagat
     1561 ctttacgctt atacggttgg atttcgcccg gtttgcgagt tttcagcaat tttaatatcc
     1621 aggtgtattg ttctggtcgc ggaccaacaa aaatctcgac ttcttcattc atccgccgcg
     1681 caatcgtatg atcatccgcc tctaacagat catccatcgg tgggcgcacc tgaatcgtca
     1741 gacggtgcgt cttgccatca taaatcggaa acagcggaac aacgcgcgca cggcacactt
     1801 tcatcaaacg accaatcgcg ggcaacgtcg ctttataggt ggcaaagaaa tccacaaatt
     1861 cgctgtgctc tgggccatga tcctgatcgg gtaaataata tccccagtac ccctgacgta
     1921 ccgactggat gaatggttta ataccgtcat ttctcgcatg cagacgaccg ccaaagcgac
     1981 gacgcaccgt gttccagaca taatcaaaaa ccgggttgcc ctgattatgg aacatcgctg
     2041 ccattttctg cccttgcgag gccatcagca tggcaggaat atcgacggcc caaccgtgcg
     2101 gcaccagaaa gataactttc tcgttattac gcctcatctc ttcgatgatc tccagccctt
     2161 gccagtcaac gcgcggctga attttctccg gtccgcgtat tgccaactca gccatcattg
     2221 ccatcgcttg cggcgcggtg gcaaacatct catcaacaat cgcttcgcgt tcagcttcac
     2281 tacgttctgg aaagcagagc gacagattga ttaacgcacg acggcgtgag ctttttccca
     2341 gtcgtccggc aaaacgtccc agccgtgcca gaatgggatc acggaacttt ggcggcgtta
     2401 aagcgatacc cgccatcgct gctacaccca gccatgctcc ccagtagcgc gggtggcgaa
     2461 aggatttatc aaactcagga atgtattcgc tattattttt tttcgtttcc atgcttttcc
     2521 agtttcggat aaggcaaaaa tcaatctggt gatagtatag cggcgcaact tgccccgcac
     2581 caaataaaaa aagccgatac tgactgcgta ccggctgcga atggatgtta attaatcaaa
     2641 ccgtagctgc ggcacaatct ctttggcctg cgccaggaat tcgcgacgat cggagccggt
     2701 cagcccttcg gtacgcggca gttttgccgt cagcgggttt acggcctgct ggtttatcca
     2761 tacttcatag tgcagatgcg gcccggttga acgtccggta ttaccggaaa gcgcgatacg
     2821 gtcgccacgt ttcaccttct gtcccggttt caccagaatc ttgcgcaagt gcatataacg
     2881 cgtggtgtag ctgcgaccat gacgaatagc cacataataa cctgctgcgc cactgcgttt
     2941 ggcaaccacc acttcaccgt cacccactga aagcactggc gtaccctgcg gcatggcgaa
     3001 atcaacacct ctgtgtgctg caacgcgacc ggtcaccgga ttagtacgac gcgggttaaa
     3061 gttagacgag atacggaact gtttcgccgt cgggaatcgc aagaatcctt tcgccagacc
     3121 agtaccgtta cgatcgtaga atttgccatc ttcagcgcgg atagcgtaat aatctttacc
     3181 ttctgaacgc aaacgtacgc ccagcagctg gctttgctca cgtttaccat caagcatttc
     3241 tcgagacatt aacaccgcaa attcatcgcc ttttttcagt ttgcggaaat ccatttgcca
     3301 ctgcatggct ttaatcactg cgctcacttc ggcgctggtt aaaccggcgt ttctggcgct
     3361 ggcaacaaag ctccccccga cggtaccttt cagcagattg ttaacccact ctccttgctg
     3421 catttcgctg gtcattttaa aaccgttagc ggcagtacgg tcataggttc gggtttcacg
     3481 acgagacact tcccaggtga ggcgctgcag ttcgccgtcc gcggttaatg tccaggagag
     3541 ttgttgaccg attttcaggt tacgcaattc tttgtcggcc gcagccagtt gggtgatatc
     3601 acccatatca ataccatact gattgagaat gctgcttagc gtatcgccag tggaaacaac
     3661 atattcatgc acgcccgctt caccggcgat tttgtcatcc agttcgtcct ggggaatggc
     3721 ttcatcttct tgtgcagctt gatcaatcgg ctcactggct tcaggtaaga gcgaacgaat
     3781 ttcgttctgt tccagctcaa tggttttgac aattggcgtg gcatcgcggt gataaacata
     3841 gggccgccag acagcgacgg ccagagtaag aacggtgagc gaccccaaca taacgcggtg
     3901 tggtcgcggt aaattattaa acgccagggc gacagagcgg gctatctgtt gcacgtaatc
     3961 acttcctcat taatctcctt tcaggcagct cgcatactgg ttggctaatt gactcaggaa
     4021 ttctgaatag cttgttttac ccagtttgat attcgtcccc aggggatcca acgttcccat
     4081 acgaacggat gtccctcgtg cgacgctctc aacgaccgct ggcctgaact gtggctcagc
     4141 aaaaacgcag gttgcttttt gctcaaccaa ctgtgttctt atttcatgta aacgctgcgc
     4201 gccaggttga atctcagggt taacggtaaa atgaccaagc ggtgtcagtc cgaactgttt
     4261 ttcgaaatag ccgtaagcat cgtgaaaaac gaaataacct ttccccttga gcggcgcgag
     4321 ctcgttacca acctgcgttt cggttgaggc taattgtgcc tcaaaatcct tcaggttggc
     4381 gtcaagtttg gctcgacttt gcggcataag ttccactaat tttccatgga ttgcaaccgc
     4441 tgtagcccgc gctatctctg gggaaagcca aagatgcatg ttgaaatcgc cgtgatggtg
     4501 atcttcgtca cttttttccg cgtggtcgtg atcatcatca tcgccgtgaa tacttttcat
     4561 cagtagcggt ttcacatctt caagctgcgc aatcgttacc tgcttcgctc ctggtaattt
     4621 gcttaccggt ttttgcataa acgcttccat ctccgggcca acccaaacga ctaagtccgc
     4681 gttctgtaag cgttttacat ccgatgggcg cagtgaataa tcatgttctg aagcgccgtc
     4741 aggaagtaaa acctctgttt ctgttacccc atcagcaatg gcagaagcga tgaacccaac
     4801 gggtttaagc gaagcgacaa cggcggcatc tgcggcctgt gttgcacctc cccagagagc
     4861 ggcggataat gctgcgaaaa gaagcgtttt tttatgtaac ataatgcgac caatcatcgt
     4921 aatgaatatg agaagtgtga tattataaca tttcatgact actgcaagac taaaattaac
     4981 atgacaagtc tggtttcctt ggaaaatgtc tcggtttctt ttggccaacg ccgcgtcctc
     5041 tctgatgtgt cgctggaact taaacctgga aaaattttga ctttacttgg gccaaatggc
     5101 gcaggtaagt cgacactggt acgggtagtg ctcgggctgg taacacccga tgaaggggtt
     5161 atcaagcgca acggaaaact gcgcatcggc tatgtaccgc agaagctgta tctcgacacc
     5221 acgttgccac tgaccgtaaa ccgtttttta cgcttacgcc ctggtacaca taaagaagat
     5281 attttgcctg cactgaaacg tgtccaggcc gggcacctga ttaacgcacc gatgcaaaag
     5341 ctctctggtg gcgaaacgca gcgtgtactg ttagcgcgag cattgttaaa tcgcccgcaa
     5401 ttattagtgc tggatgaacc cactcaaggc gtggatgtaa atggccaggt ggcgttatat
     5461 gaccttattg accaactgcg tcgtgaactg gattgtggcg ttttaatggt ttctcacgat
     5521 ctgcatctgg tgatggcaaa aaccgatgaa gtgctgtgcc tgaatcacca catttgttgt
     5581 tccggcacac cggaagttgt ttccctgcat ccggagttta tttcaatgtt tggtcctcgt
     5641 ggtgctgaac aactgggtat ctatcgccat catcataacc atcgtcacga tttacaggga
     5701 cgaattgttt tgcgtcgggg aaatgatcgc tcatgattga attattattt cccggttggt
     5761 tagccgggat catgctcgcc tgtgccgcgg gtccgctggg ttcgtttgta gtctggcgtc
     5821 gtatgtctta tttcggtgat acgctggctc atgcctcatt acttggtgtc gcgtttggtt
     5881 tgttgctgga cgtgaatcca ttctatgcgg tgattgccgt tacgctgctg ctggcgggcg
     5941 gtctggtatg gctggagaag cgtccacagc tggcgatcga cacgttatta gggattatgg
     6001 cgcacagtgc cctgtcgctg ggcctggtgg tcgttagtct gatgtctaat attcgtgttg
     6061 atttgatggc ttacctgttc ggtgatttgc tggcagtgac gccagaagat ctcatctcta
     6121 ttgcgattgg cgtggtcatc gtggtggcta ttttgttctg gcaatggcgc aatttgctgt
     6181 cgatgacgat tagcccggat ctggcgtttg ttgatggtgt gaaattacag cgcgtgaaat
     6241 tgttgttgat gctggtgacg gcattgacga ttggtgtagc gatgaaattt gtcggtgcgt
     6301 tgattattac ttcgttgctg attattcctg cagctactgc gcgtcgcttt gcccgtacgc
     6361 cggaacagat ggctggtgtc gctgttttgg tggggatggt ggcagtaact ggcggtttaa
     6421 ccttttccgc gttttacgat acgccggcgg gtccgtcggt agtgctatgt gctgcactgt
     6481 tatttattat cagtatgatg aaaaagcagg ccagctaatc tgtcgctgaa cacatttgtc
     6541 ggatgcggcg cgagcgcctt atccgaccta cggttcgcta tctctggtag gccggataag
     6601 acgcgaatag cgtcgcatca ggcacactgc caatgtcgga tgcggtgcga gcgaccaatc
     6661 cgacttacgg catttctggc ggcgttatgc caaagtgatt ccacgcccgc gtcgtcgcca
     6721 tacgcccacg cggtgtacgc tgcaaaaagc cttgctgaat cagataaggc tccagcacat
     6781 cctcaatggt ttcacgttct tcgccaatgg ctgccgccag gttatccaga cctaccggcc
     6841 caccaaagaa cttatcgatt accgccagca acaatttgcg gtccatataa tcgaaacctt
     6901 cagcatcgac attcaacata tccagcgcct gagcagcgat atctgccgag atggtgccat
     6961 cgtgcttcac ttcggcgaaa tcacgcactc gacgcagcag acggttggca atacgtggcg
     7021 taccgcgagc gcgacgagca acttccagcg cgccgtcatc actcatctca agccccataa
     7081 agcgtgcgct gcgactgacg atatattgca gatccggcac ctgataaaac tccagacgtt
     7141 gcacaatacc aaaacggtcg cgcaacggtg atgtcagcga acctgcgcgc gtggttgcac
     7201 caatcagggt aaacggcggc aaatcaattt taatggagcg tgccgccgga ccttcaccaa
     7261 tcatgatatc cagttggtag tcttccattg ccgggtacag cacttcttca acaactggcg
     7321 atagacggtg gatctcatca ataaacagca cgtcatgcgg ttcaaggtta gtgagcatcg
     7381 ctgccagatc gcccgccttt tccagcaccg gaccagaagt cgtgcgtaaa ttaacgccca
     7441 tttcattggc gacaatgttg gcaagcgtag ttttacccaa ccccggagga ccaaaaatca
     7501 acagatgatc gagggcatcg ccgcgcagtt tcgctgcttt gatgaaaatc tccatctgtg
     7561 aacgaacctg cggctgacca acatactctt ccagtaattt agggcgaatg gcgcgatctg
     7621 ccccatcttc cggcaaagtg gtaccggcag aaatcagacg gtctgcttca atcatccttt
     7681 acctcataac gcggcgcgta gggcttcgcg aattaatgtt tcactgctgg cgtcagtgcg
     7741 agcgattttg ctcaccatgc ggcttgcttc ttgtggttta tagcccagcg ccaccagcgc
     7801 ggcaaccgct tcctgttcag catcgtcggt tgccgggctg gcaggcgacg tgagcaccag
     7861 atcggcggct ggcgtaaaga gatcgccgtg caaaccttta aatcggtctt tcatttcgac
     7921 aattaaacgc tcggcggttt ttttaccaat gcccggcagt ttcaccagtg ctcccacttc
     7981 ttcacgttca acggcattaa cgaactgctg cgctgacatt ccggagagga tcgccagcgc
     8041 caacttcggg ccgacgccgt tggttttgat taactcttta aacaacgtgc gctcttgttt
     8101 attgttaaaa ccgtacagca gttgcgcgtc ttcacgcacc acaaagtggg tgaaaacgat
     8161 cgcttcctga cccgcttcag ggagttcata aaaacaggtc atcggcatat gcacttcata
     8221 gcctacgccg cccacttcaa ttaacaccag cgggggttgt ttttcaatga tgatgcctct
     8281 gagtctgcct atcacatgac gctcctgcgt aatgaatcaa agataatgct gtatgataaa
     8341 aaaatgctgg atagatatcc agcgaaggat gaagaaaact tgcgaggtgt ctcgatgaac
     8401 tgaaaaatgg cacagtataa tttattctac aggttatatt ggaagcaaat attttaatat
     8461 tacatattca gtgaataaat gcgtaataaa aatatacatt gcgcctcctg aaaaaaaata
     8521 aattttttat gctattacgt atattcgtat ctatttcaat ggaatgacaa cgtgaatatt
     8581 aattaccctg ctgaatatga aattggagat atcgtcttta catgtatagg tgctgcctta
     8641 tttggtcaaa tatcagctgc atcaaattgc tggagtaatc acgtcgggat cattatcggt
     8701 cataacggtg aagacttcct ggttgcagaa agccgggttc ccctttcaac catcaccacg
     8761 ctatctcgtt ttattaaacg ttcgtctaat caacgctatg ctataaagcg attagacgct
     8821 gggctgacag aacaacaaaa acaacgaatt gttgaacagg ttccctcccg gctacgcaaa
     8881 ctttaccaca ccggttttaa atacgaatct tcgcgccagt tctgttcaaa atttgttttt
     8941 gatatttata aagaagcgct atgcattccg gtgggtgaaa tagaaacgtt tggagaactg
     9001 ttaaatagca atccgaatgc aaaactcact ttctggaaat tctggttctt aggttctatt
     9061 ccgtgggagc gtaaaaccgt cacgccagcc agtttgtggc atcatccggg tctggtgttg
     9121 attcacgcgg agggagttga aacgcctcag cctgaactga ccgaggcggt ataacttaac
     9181 gcagtcgccc tctcgccagg ttcagccgcg attcgctcat ctgcattgca ttctggctga
     9241 cgtgacaatg ggtaatcgcg atagctaacg catcggcggc atccgcctgt ggattagcgg
     9301 gcagtttcag caaggtgcgg accatatgct gcacctggct tttttcggca ctaccaatac
     9361 ctaccaccgt ttgctttacc tgacgtgccg cgtattcaaa taccggcaat tcctgattca
     9421 ccgccgccac aatcgccacg ccgcgcgcct gccccagttt cagagccgag tcggcatttt
     9481 tggccataaa gacttgttca atggcgaaat aatctggctg gaactgggtg atgatttccg
     9541 tcacgcccgc atagatgagc ttcagacgag acggtaaatc atccactttg gtgcgtatgc
     9601 atccgctacc caggtaggac agttgcctgc ctacctggcg gatgacacca tagccgatca
     9661 cgcgcgaacc cgggtcaatg ccgagaataa tagccatcac gcgtctccgt tttgctgttt
     9721 agcaggcccc atcagagagt tgctgcaacc tcatcagaga tttcaccgtt atggtagact
     9781 tcctgcacgt cgtcgcaatc ttccagcata tcgatcagac gcatcagttt cggtgcggtt
     9841 tctgcatcca tatcagcttt ggtggacggg atcatggaaa cttccgcgct gtctgctttc
     9901 agacctgccg cttccagagc gtcgcgcact ttacccattt cttcccatgc agtgtagacg
     9961 tcaatcgcgc cgtcatcata ggtcacaacg tcttcagcac ctgcttccag tgctgcttcc
    10021 ataatggtgt cttcatcgcc tttctcgaag gagatcacgc cttttttgct gaacagatag
    10081 gcaacggaac catcagtacc gaggttaccg ccacatttgc taaatgcatg acgcacttca
    10141 gcaacggtac ggttgcggtt gtcagacaga cattcaatca tgattgccgt gccgccagga
    10201 ccgtaacctt cgtagatgat ggtttccatg tttgcatcat catcaccgcc cacaccacgt
    10261 gcaattgcgc ggttcagtgt gtcacgggtc atgttgttag acagtgcttt atccaccgcc
    10321 gcacgcagac gcgggttagc gtccggatcg ccaccgccca gcttagccgc ggttaccagc
    10381 tcacgaatga ttttagtgaa gattttaccg cgcttagcat cctgcgcagc tttacgatgt
    10441 ctggtgttgg cccatttact atgacctgcc ataaaaatat ctccagatag ccctgcctgt
    10501 tcaggcagcg ttaattacaa actgttcaat cgcctgccgg ttgctccagg acttagtgag
    10561 cgccgcggca gcaggcgcat caagccactt gtaagccaga tgttcagtga agactacctg
    10621 gcgttcgtgc gggagcgcaa gacagaacca tgattccgta ttacgcgtca cgcccggcgc
    10681 atagcgatga cgtaaatgtg aaaaaatttc aaactctacc gtgcgctgac agtcaattaa
    10741 ggtcagttgt tcagcgacaa catcaatggt gacctcttcc tttacttcgc gcatggcagc
    10801 ttgcggcgcg gtttcaccct cttccacgct gccggttacc gactgccaga aatcgggatc
    10861 gtcacgccgc tgcaacatca gcacccgttt cgtatcttgt gcatagatga ccactaagat
    10921 cgaaacggga cgcttataca ctttatcctt cacgctgcct cttcgttgac tgccttcgct
    10981 catcccattc acatagttat ctatgctcat gggagttcac tcagttgccg ccttgatgca
    11041 acgcgaatga tttcgtgtat ttgagtcata tcagttattc tcagccttct tcacaacctg
    11101 aatgctcagc tcagccagta cagtcgggtt agcaaagctc ggtgcttcag tcatcagaca
    11161 cgctgccgcc gtggttttcg ggaaggcgat aacgtcacgg atattgtcgg tgccagtcag
    11221 cagcatggtc agacggtcaa gaccgaatgc cagacctgcg tgcggcggag taccgtattt
    11281 cagggcgtcg agcaggaagc cgaatttctc gcgctgttcc tcttcgttga tacccagaat
    11341 accaaacacc gtctgctgca tatcaccatt atggatacgt actgaaccac cgcccacttc
    11401 gtaaccattg atgaccatat cgtaagcgtt cgccaccgca ttttccggtg cagctttcag
    11461 ttctgcagca gtcatgtctt tcggtgaggt gaacggatgg tgcattgccg tcaggccgcc
    11521 ttcaccgtcg tcttcaaaca tcgggaagtc gataacccac agcggtgccc atttgctttc
    11581 gtcggtcaga ccaaggtctt tacccacttt caggcgcagt gcgcccatcg cgtcggcaac
    11641 aattttcttg ttgtcggcac cgaagaaaat catatcgcca tcttgcgcgg cagtacgatc
    11701 caggatggct tcgatgattt ctgcattaag gaacttcgct accgggctgt tgataccttc
    11761 cagacctttc gcgcgttcgt taactttgat gtaagccaga cctttcgcgc cgtagatttt
    11821 aacgaagtta ccgtattcgt cgatctgctt acgggtcagc gatgcgccac ccggaacacg
    11881 tagagcggca acacggcctt tcggatcgtt cgccggcccc gcaaatactg caaactcaac
    11941 agatttcagc agatcggcaa cgtcggtcag ttccatcggg ttacgcagat ccggtttatc
    12001 agaaccataa cggcgttctg cttccgcaaa ggtcattacc gggaaatcgc ccagatccac
    12061 gcccttcact tccagccaca aatgacgcac cagcgcttcc atcacttcac gcacttgcgg
    12121 cgcggtcatg aaagaagttt ccacatcgat ctgagtaaat tcaggctgac gatcagcgcg
    12181 caggtcttcg tcacggaagc atttaacgat ctgatagtaa cggtcaaaac cggacatcat
    12241 cagcagctgt ttgaacaact gtggggattg cggcagtgcg tagaatttac ctttgtgcac
    12301 acgagaaggc accaagtagt cacgcgcgcc ttccggcgtg gctttggtca gcatcggagt
    12361 ttcgatgtcg aggaagccgt ggtcatccat aaaacggcgc accaggctgg tgattttagc
    12421 gcgggttttc aggcgctgcg ccatttccgg gcgacgcagg tcgaggtagc ggtatttcag
    12481 acgcgcttct tcggtgttga cgtggttaga gtcaagcggc agaacatctg cgcggttgat
    12541 gatagtcagc gaggacgcca gcacttcaat ttcgccggtc gccatatcgc ggttaatatt
    12601 tttttcgtca cgcgcacgta cggtgcccgt aacctgaatg cagaactcat tacgcagttc
    12661 agaggccagc tttaacgcgt ccgcacgatc cggatcgaaa aatacctgca cgataccttc
    12721 gcggtcgcgc atatcgatga agatcaggct accaagatca cgacgacggt tgacccaacc
    12781 acacagagtc acctgctgcc ccacgtggga caaacggagc tgtccacaat attctgtacg
    12841 catgagatat cccttaactt agctgccggc ggatgccccc tgctgcgcag gtgaccaagt
    12901 cgcagcgtta gctgtatgtc acaactgaat gaaaaaaggc ggctattata ctggaaattc
    12961 tgccgcaccg taagagcctg gcccgcgctg taacgcctcg ttaccacttt atatcgggcc
    13021 tgaaatcaga ctctacgcca gtttgctata aaggtgttgc ccgaactcat aaaaattaac
    13081 aaaatttgtc gttccgccat cggctaatcg cattaaggtg tgaggcacga ttttgttttt
    13141 tcaggagtca gcatgcttga acttaatgct aaaaccactg cgttggtggt gatcgattta
    13201 caagaaggca tcttaccttt tgccggtggt ccacatactg ccgatgaggt agttaatcgc
    13261 gccgggaagc tggcggcgaa atttcgcgcc agcggtcagc ccgtgtttct ggtgcgagtt
    13321 ggctggtctg ccgattacgc cgaagcatta aaacagccgg ttgatgcccc ctcccccgct
    13381 aaagtgttgc ccgaaaactg gtggcaacat ccagctgcat taggtgcaac tgacagtgat
    13441 atcgaaatca tcaaacgtca gtggggtgcg ttttacggta cggatctgga gttgcaactg
    13501 cgccgccgag gtatcgatac aatagtgtta tgtgggatct cgaccaatat cggtgttgaa
    13561 tccaccgccc gcaatgcctg ggaacttggc tttaatctgg tgattgccga agacgcctgt
    13621 agtgccgcca gcgccgagca acacaataac agcattaatc atatctaccc gcgcatcgcc
    13681 cgtgtgcgta gcgtggaaga gatccttcac gcgttatgat ttacatcggt ctaccgcaat
    13741 ggtcgcatcc taaatgggtg cggttgggga tcaccagcct tgaagagtat gcccgccact
    13801 ttaactgcgt gatacgggat ttttaaaaat cactaaagaa cgcccaagag catgtgtttt
    13861 ctttagaata atcaaagcgt tggattgcca tgccatatga aaatcggcaa agttcatatg
    13921 taacaaagtg tcccaatcat gccccaaaat gccccacaac aaatattttt gccccatgca
    13981 tgccccaaaa aatcactttc cctcactctt gacactgttt atccatacag ttaaaaataa
    14041 tactgtataa aaacacagtg tagagggatt tttatgcgta ttgaaatctg catagccaaa
    14101 gagaaaatga ctaaaatgcc aaccggtgct gtggatgcgt taaaggaaga attaacccga
    14161 cgcatcagta aacgttatga cgatgtagag gtgatcgtaa aagccaccag caacgatggc
    14221 ctttctgtta cgcgcaccgc cgataaagat tcagctaaaa cttttgttca ggaaactctg
    14281 aaagatacct gggagtctgc tgacgagtgg tttgttcact aattagcacg taaaatcagt
    14341 aacggctgga aatcattcaa tactcgcact atcggaagtt caccagccag ccgcagcacg
    14401 ttcttgcata cgacgtgtct gcggtctcaa ctccgcccgc ccccgccaat catgattggg
    14461 cagtaccagt acagcactag caaagacagg aaattagcag tctcagcaaa acaccgacca
    14521 gacggtgaga agacaaaaag atacgcaaag atgccgcggt tccagaagaa taaaaaaccg
    14581 ctaatggaca gcctccttaa cacagttttt tatatacatg attcactgat accagtggca
    14641 ttaatagcta ttatatagct tcagctcctt ttttcatacc ttaaggaaaa attgccccct
    14701 cccccaaaaa gtatcgtttt tttacattaa accgatccag tttaggatag tgtaaagcag
    14761 aagttcttca atatataaca tatggagtta atatgcttac acaaactata tattcgcctc
    14821 ctccagaagt tcctgtctac tgctgaaggg aaccaataac attgcaaagc agataaaaaa
    14881 gaatccaaca cctctcaaca taaagaatac gccctgatat ttatgctccc gacaaataac
    14941 aatcacagat taatttcaaa ttcgttctcc acttattcaa tcgacactag ccgtgcatat
    15001 gaaaattatc taacccattg gactgaatgg aaaaataacc gcatacaaga agaacaacga
    15061 gacatcgctt ttcagcgact agtatcatgt ctacaaaacc aagagacgaa cctggacttg
    15121 tctgaattag gcctgacaac attacctgaa atccccccgg aaattaaatc aattaatata
    15181 agtaaaaata atttaagctt aatctcccca ttgcctgcgt cccttacaca gcttaatgtc
    15241 agctataaca gacttattga actgcctgct ttgcctcaag gacttaaatt attgaatgcg
    15301 tcccacaatc aactaatcac actacccaca ctccccatat ctttgaagga gcttcatgtc
    15361 tcaaataatc aattatgttc tcttcctgtt ttaccagaac tactggaaac attagatgta
    15421 tcatgtaatg ggctggcagt tttaccacct ttaccatttt ctttacaaga gattagcgca
    15481 atagggaatc ttcttagtga actcccccct ctacctcaca acattcactc catatgggca
    15541 atcgacaata tgttaaccga tattccatac ctgccggaaa atttaaggaa cggttatttt
    15601 gacataaatc agataagtca tatcccggaa agcattctta atctgaggaa tgaatgttca
    15661 atagatatta gtgataaccc attgtcatcc catgctctgc aatccctgca aagattaacc
    15721 tcttcgccgg actaccacgg cccgcagatt tacttctcca tgagtgacgg acaacagaat
    15781 acactccatc gccccctggc tgatgccgtg acagcatggt tcccggaaaa caaacaatct
    15841 gatgtatcac agatatggca tgcttttgaa catgaagagc atgccaacac cttttccgcg
    15901 ttccttgacc gcctttccga taccgtctct gcacgcaata cctccggatt ccgtgaacag
    15961 gtcgctgcat ggctggaaaa actcagtgcc tctgcggagc ttcgacagca gtctttc
//
LOCUS       DADMKF010000090        15961 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.092, whole genome shotgun sequence.
ACCESSION   DADMKF010000090 DADMKF010000000
VERSION     DADMKF010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15961
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.092"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(<1..453)
                     /gene="spy"
                     /locus_tag="JNC24_002635"
     CDS             complement(<1..453)
                     /gene="spy"
                     /locus_tag="JNC24_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone"
                     /protein_id="HAY9159347.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIREIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPA"
     gene            complement(783..1751)
                     /gene="astE"
                     /locus_tag="JNC24_002636"
     CDS             complement(783..1751)
                     /gene="astE"
                     /locus_tag="JNC24_002636"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HAY9159348.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGDPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSARHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(1744..3087)
                     /gene="astB"
                     /locus_tag="JNC24_002637"
     CDS             complement(1744..3087)
                     /gene="astB"
                     /locus_tag="JNC24_002637"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HAY9159349.1"
                     /translation="MNAWEVNFDGLVGLTHHYAGLSFGNEASTRHRFQVSNPRLAAKQ
                     GLLKMKTLADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVARQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHLTVANLNNKFHRSLEAPVTESLLKAIFNDEEKFS
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGMQLFVYGREEGNDTRPSRYPARQTREAS
                     EAVARLNQVNPQQVIFAQQNPDVIDQGVFHNDVIAVSNRQVLFCHQQAFARQSQLLAN
                     LRARVNGFMAIEVPATQVSVSDAVSTYLFNSQLLSRDDGSMMLVLPQECREHAGVWGY
                     LNELLAADNPISELKVFDLRESMANGGGPACLRLRVVLTEEERRAVNPAVMMNDTLFN
                     ALNDWVDRYYRDRLTAADLADPQLLREGREALDVLSQLLNLGSVYPFQREGGGNG"
     gene            complement(3084..4562)
                     /gene="astD"
                     /locus_tag="JNC24_002638"
                     /pseudo
     CDS             complement(3084..4562)
                     /gene="astD"
                     /locus_tag="JNC24_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416260.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
     gene            complement(4559..5593)
                     /gene="astA"
                     /locus_tag="JNC24_002639"
     CDS             complement(4559..5593)
                     /gene="astA"
                     /locus_tag="JNC24_002639"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HAY9159350.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRVDFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLARTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            complement(5590..6810)
                     /gene="astC"
                     /locus_tag="JNC24_002640"
     CDS             complement(5590..6810)
                     /gene="astC"
                     /locus_tag="JNC24_002640"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
                     /protein_id="HAY9159351.1"
                     /translation="MSQPITRENFDEWMIPVYAPAPFIPVRGEGSRLWDLQGKEYIDF
                     AGGIAVNALGHAHPELREALNEQASKFWHTGNGYTNEPVLRLAKKLIDATFADRVFFC
                     NSGAEANEAALKLARKFAHDRYGSHKSGIVAFKNAFHGRTLFTVSAGGQPAYSQDFAP
                     LPPDIRHAAYNDINSASALIDDATCAVIVEPIQGEGGVVPASNAFLQGLRELCDRHNA
                     LLIFDEVQTGVGRTGELYAYMHYGVTPDLLTTAKALGGGFPVGALLTTEECASVMTVG
                     THGTTYGGNPLASAVAGKVLELINTPEMLNGVKQRHDWFVERLNTINHRYGLFSEVRG
                     LGLLIGCVLNADYAGQAKQISQEAAKAGVMVLIAGGNVVRFAPALNVSEEEVTTGLDR
                     FAAACEHFVSRGSS"
     gene            7256..8062
                     /gene="xthA"
                     /locus_tag="JNC24_002641"
     CDS             7256..8062
                     /gene="xthA"
                     /locus_tag="JNC24_002641"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HAY9159352.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGLVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            8229..8939
                     /locus_tag="JNC24_002642"
     CDS             8229..8939
                     /locus_tag="JNC24_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAY9159353.1"
                     /translation="MNAERKFLFACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFIIATLFVLPGSILVIAGGIVFGPLLGTLLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGLTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILVQLAKLYARHKH
                     VDLSASRRSPLTHPKNEG"
     gene            8944..9621
                     /gene="ydjY"
                     /locus_tag="JNC24_002643"
     CDS             8944..9621
                     /gene="ydjY"
                     /locus_tag="JNC24_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdjY"
                     /protein_id="HAY9159354.1"
                     /translation="MSQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMAYATPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKRGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            9636..10343
                     /locus_tag="JNC24_002644"
     CDS             9636..10343
                     /locus_tag="JNC24_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAY9159355.1"
                     /translation="MMKMQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAADLTSIRFRSFFIATGLGQLPTTIVYSWAGSMLTGGTFWFVTGLFILFALTV
                     VIFMAKKIWLERQKRND"
     gene            10343..10891
                     /locus_tag="JNC24_002645"
     CDS             10343..10891
                     /locus_tag="JNC24_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HAY9159356.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSSDKLLAVAD
                     WRQNPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            10901..12067
                     /locus_tag="JNC24_002646"
     CDS             10901..12067
                     /locus_tag="JNC24_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAY9159357.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVDLLWVNGE
                     NFRTLKEANLLQTGWAETLPNWRYVDTQLPVREDFSVPTQGAESPWGGAQLTFIARRD
                     VTPQPPQTPQALLEFAKANPGTVTYPRPPDFTGTAFLEQLLIMLTPDPAALKEAPDDA
                     TFARVTAPLWQYLDALHPYLWREGKDFPPSPARMDALLKAGTLRLSLTFNPAHAQQKI
                     ASGDLPASSYSFGFREGMIGNVHFVTIPANANASAAAKVVANFLLSPDAQLRKADPVV
                     WGDPSVLDPQKLPDGQREILQSRMPQDLPPVLAEPHAGWVNALEQEWLRRYGTH"
     gene            12040..13576
                     /locus_tag="JNC24_002647"
                     /pseudo
     CDS             12040..13576
                     /locus_tag="JNC24_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416269.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            13576..14229
                     /locus_tag="JNC24_002648"
     CDS             13576..14229
                     /locus_tag="JNC24_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAY9159358.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALL
                     STLKGTARRNAVKDALDRAGLAETYHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRELAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            14296..15603
                     /locus_tag="JNC24_002649"
     CDS             14296..15603
                     /locus_tag="JNC24_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HAY9159359.1"
                     /translation="MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAID
                     TRPSAFYNGWPQTLNGPSGHEPAALNLSASWLDKMSTEQLNAWIKQHNLKADAPVALY
                     GNDKDVDAVKTRLQKAGFTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
                     TAKPAGDWKVIEAAWGAPKLYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKH
                     GIRHDTTVILYGRDVYAGARVAQIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKV
                     KAEPDFGVKLPAQPQLMLDMEQARGLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGE
                     IAGARWGHAGSDSTHMEDFHNPDGTMRSADDITAMWKAWNIKPEQQVSFYCGTGWRAS
                     ETFMYARAMGWKNVSVYDGGWYEWSSDPKNPVATGERGPDSSK"
     gene            complement(15612..>15961)
                     /locus_tag="JNC24_002650"
     CDS             complement(15612..>15961)
                     /locus_tag="JNC24_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9159360.1"
                     /translation="FYALVPFGFILAAPEQNALAGGWLLFAFIGTGSSFLAFAALAAK
                     HQIDNPGYAHKSFYYLGGLTEGTETILLFVLGCLFPAWFAWFAWIFGALCWMTTFTRV
                     WSGYLTLKSLQRQ"
BASE COUNT         3701 a         4345 c         4162 g         3753 t
ORIGIN      
        1 cgctggacgt tctgtcagac gcttctcaaa attagcatta aattgctttt tctgttccgg
       61 cgtcaggatg ttgtaaattt tgttctgggt ttccatgtgc gccagcatgt tagctttgcg
      121 ctgttcttcc atttttgcga tctgcgcttc agcttttgct ttatcgaagg tatcgctggc
      181 aatgatgtca tgcattgcgc ggcgttcttc cagcggcgga cgtttcatct ggtcacgctg
      241 gcctttcatg atttcgcgga tctgctgttt ctgcgcgtcg gtcaggttca ggtctttgaa
      301 catcatgtcc tgatgcggac cgaacttgcc tttgtggtgc atcatcggct tcgcgtcagc
      361 cggtgctgcg gtagtggtgt ctgcggcatg ggccaggtta gccgcgccaa gagccagggt
      421 agaggcaaca aacagtgcag ttaatttacg catattctat atccttcctt tcagttattt
      481 attacggctt tcttaagtag cgtgccgtgt tgacgagatt aactttactg actttagcgt
      541 caattaatca gagcaacggt aaaacaatga aagtgtaaaa aacacttttg cgccaattat
      601 ggagaaaaaa agaaaatttg atggagagtg atgagagaat attacaacac aatgattttg
      661 cagagattat gaagaactat accggatgac tggtgataaa taaagcaaat aaccaggatt
      721 aatctgtatt aatttataag aaagcaactt aatacccgca gaatgatttc tgcgggtaag
      781 tattagctta ttttttcgag cattaatccc gcgcgtaatc ccaacgctac caacggatta
      841 gggaataaca catactctac atcatgggtt acggtaaaac gttcctctcc gtcctgcgcc
      901 agcaatgttc ctttctcaaa cggcataaaa ttcagcgtgt cacttgccat atgcatttcg
      961 aaggacggcg agtggcgagt aatttgcgaa accacccgat aacggagcgg cggtgttctc
     1021 acgataccga cactctcacc agatagcagc gcagcaattg cgctggcagt tactgcaaac
     1081 tggcgaagat cgttttgccc aaagggcaac gctttgccaa gttccagcgt acaggccagc
     1141 gcgccaaaat gtctggcgct gaaatgggta aacgtaccac caggctcctg atggaacacc
     1201 agcgcctcca gccccgccgc acccagccac gtcagaaatt tctcgtccca gggaatgtcg
     1261 cgttgcggta atacaccgaa ctgaggatgc aaggagccac gaattgcggt atgtagatca
     1321 aggtgccaac gcacagattc tttgccctgg tcataaaaat cttccaggca ctgttccagt
     1381 tcgcgcgccc gacaggtttc tccgctttca gcaaatagct gccaacgacc gccaaacatt
     1441 cggttcatat cgctatggca ataacgtttc ccttgcttca gcgcaggagg atccccgagg
     1501 atcaccagca accgccaacg taacgggatc tcgccgtgag atatcgcgcc aagcaacgcg
     1561 tccagcattt ccacaggtgc cgtctcatta ccgtggatcc ccgcagaaat caccagcgcg
     1621 ccttgcggtg gcgttaatgg cgtcagttcc agcacaccat cgcccagcca gcgccagcga
     1681 acgccgttga tttctcgctc ggtgataacc ggttttttac ccgttaaggt cagagcaaga
     1741 aaattatcca ttgccccctc cctcgcgctg gaacggataa accgaaccga gattcagtaa
     1801 ttggctcaat acatccagtg cttcccgccc ttcgcgcagc aattgcgggt cggccagatc
     1861 ggcagcagta aggcgatcgc ggtagtaacg atccacccag tcattgagcg cattaaacag
     1921 cgtatcgttc atcatcaccg ccggattcac cgcccggcgt tcttcttctg tcaatactac
     1981 ccgcaaccgc aggcacgccg ggccgccgcc attcgccatg ctttcacgta aatcaaagac
     2041 ttttagttcg ctaatcgggt tgtcagcggc aaggagttca ttgagataac cccatactcc
     2101 ggcgtgttcc cgacactcct gaggcagcac caacatcatg gaaccatcat cgcggctcaa
     2161 cagttggctg ttaaacagat aggtagacac cgcatccgac acggaaacct gagttgccgg
     2221 aacttctatc gccataaaac cgttcacccg cgcccgcagg tttgccagta actgtgactg
     2281 gcgagcgaac gcctgttggt ggcaaaacag cacctggcgg ttacttacgg caatcacgtc
     2341 attatgaaaa acaccctggt cgataacgtc cgggttttgc tgggcgaaaa tcacctgttg
     2401 gggattcacc tgattaagcc ttgccaccgc ctcgctggct tcgcgagtct gtcgcgccgg
     2461 ataacgggaa ggccgggtat cattgccttc ttctcgcccg tagacaaaaa gttgcatacc
     2521 cggctcaccg taatgaccgc cgagacgatt gtggtttgcc gccccctcat caccgagcaa
     2581 cgctacctgc ggcaacgccg aatggacgct aaatttctct tcgtcgttaa aaatagcttt
     2641 taacagcgat tcagtgacgg gcgcttccag cgaacggtga aatttattgt tcaggttggc
     2701 aacggtgaga tgcactttac catccagcgt atcggcagat ggcgcgatcg ttgccgcatt
     2761 ggctacccac attggcgaag cggagctgac gctggaaagc cagtgcggtg cctggcgtgc
     2821 aactttttcc agtacctgct catcgctacc actgaatccc aactgacgca gcaccggaat
     2881 aaacggacgc tcgtgcggcg ggatcacggc ctgggggaat cccgcatcgg caagggtttt
     2941 cattttcagt aagccctgtt tcgccgccag tcgcgggtta gacacctgaa aacggtgacg
     3001 ggtagaggct tcattaccaa acgacaggcc agcgtaatga tgcgtcagcc ctaccagccc
     3061 gtcgaaatta acttcccagg cgttcatcgc accacctcat cggaaaaatc cagcccgggg
     3121 ttaagcgttg cgggcaaagt taacgagtcc gactccaggc tcgccatagg ccatgcgcag
     3181 taatctgcgg cataccaggc gctggggcga tggttaccgg atgcaccaat gccgccgaat
     3241 ggcgcggtac tggcagcacc agtaagcggt ttgttccagt taacaatccc cacccgcgcc
     3301 tccagcaaca gttgatcgaa tttttcccgc tcgggggaaa ccagaccgca agagagtccg
     3361 aagcgagtgt tattcgccat tagaatcgct tcctcgaaag aatcataacg ccagacgcgc
     3421 aataacggtc cgaacacctc ttcatctggt acgccagcaa cgcctgtcat ttcaatgatc
     3481 cccggcgtca gcaacgatgt ctctgattgt aataagcgcg gcgcaagcag ggttcgtccg
     3541 cccatcgctt ccagttgctg ctaggcagta accacctgct gtgcggcctg ttcagaaatc
     3601 agcccgccaa taaacggctg cggttcgtca tcccagttgc ccggcgttaa tcgctggctg
     3661 acggcaacca gacgagcaag aaacgcatcg ccctgtgctc cgcttttgag caataaacgg
     3721 cgggcgcagg tgcagcgttg cccggctgtg acaaacgccg actgaatggt cagatggaca
     3781 gccgagtcga tatccgccac ctcatcgata attagcggat tattaccgcc catctcaagg
     3841 gcgagaattt tctccggctg accggagagc tggcgatgca gctggtagcc ggtattggcg
     3901 ctaccggtaa acagcaaacc gtcgagatcc tccagcgcac tcagcgcctg acccgtttca
     3961 cgcccgccct gcaccaggtt cagcacgccc ggcggcaagc cagcctgctg ccataaacgc
     4021 attaccgctt cgccactcca cggtgtcagt tcgctgggtt taaagataat ggtgttacct
     4081 gccagcaatg ccggaacgat atgtccgttc ggcaaatgac cagggaaatt atacggccca
     4141 aacaccgcca gcacgccgtg cgggcgatgt cgcaggctcg ccgcaccgtc cggcatttca
     4201 ctacgctgct cgccggtacg aacgtgatac gccttaattg atatcgcgat tttattgatc
     4261 atcgccgtca cttcggttgc cgcttcccag cgcggcttac ccgtttctct ggcaataatc
     4321 gcggttaatt cggctttatt gctttccagc aatccggcaa agcgttcgac aacgacctga
     4381 cgttcagcca atgagagccg cgcccagcgc ggaaacgccg cacgggctgc ccgacaagcc
     4441 tgttcgacct gagcggcatc ggcatcattg ccttgccata acacctcgcc cgataccgga
     4501 ttacgcttca cacgcgatgc gccctggccc gttatccagt caccgttaat ccataaagtc
     4561 atgctgtttt ctcctctgcg cacaggcgca ccagacgaac gcgatcccct gcgtggcatt
     4621 tgagggcatc cagttgtgcg gcggttaaaa tcaaacgctc ggttgccgga tcggtacgcg
     4681 ccagcaccac gcggaaatgg tgataatttt cattggcgac caggcaggcc ggaaaatcgc
     4741 cctgcgcagg ctgcccttct gccacttcca ccagccgact tttacggatg gcgcgcacgc
     4801 ggtcgatgtc acactcaagc gttggcccgc cgtcgaagat gtcgatatag ttgcggtaac
     4861 gaaaaccttc tttctccagc accgcgcggg caggcgcggt ttgcggatgt acctgaccga
     4921 tgacgtcctg ggcttcctgg gataaaaagt gggtatagat cggatgtttc ggcatcagtt
     4981 ctgcaataaa tgccttttgc ccggtgccgc agagaaaatc gacgcggcta aaatccatcg
     5041 aaaagaagcg tttaccgagg ctttgccaga acggtgaata gccgtgttcg tcaatcaccc
     5101 cgcgcatttc agcaaccact ttgtcattaa acttgtcgcg aaaagccgcc ataaacataa
     5161 agcgcgattt cgacagcaaa tagccgttgc cctctttgcg ccagtccggg tcgagaaaca
     5221 gcgtgcacag ctcgctgctg ccggtgtgat cgttactaag aaacagcgtc ggcaatgcgt
     5281 tatagacatt cagctctttt gaggcgtgaa ccaacgtgcc gacccgatag ttgtaccagg
     5341 gatcgttcag cccaaccgcc acctcaatgg cgcaaatccc cgccacggtg cctgtctcgc
     5401 tatcttccag cacgaacaca tagccctgct cgcttttggg cagttcgcct tgccaggttt
     5461 tgattgccct ttcgatacgc gcggaaagcg tggcttcatt ggcgggaagc gacgtcaggc
     5521 cgccgcccgt tttgctggca agctgcatca gcgccgagac atctgagcgc tcaacgggac
     5581 ggatgaccat catgatgaac ctcggctaac aaagtgttcg caagcagctg caaagcgatc
     5641 cagtccggtc gtcacctctt cttcgctgac attgagcgca ggcgcaaaac gcaccacgtt
     5701 gccacccgca atcagtacca taacgcctgc tttcgccgcc tcctgagaga tctgtttcgc
     5761 ttgtccggcg taatcggcat tcagtacaca gccaatcaac aaacctaagc cgcgaacttc
     5821 actgaacaaa ccgtagcggt gattaatggt attaagacgc tcaacaaacc agtcgtgacg
     5881 ctgtttaacg ccattaagca tctctggtgt gttgatgagc tccagcactt tgcccgccac
     5941 cgccgaggcc agcggattgc cgccataggt ggtgccatga gtgccaacgg tcatcacgct
     6001 ggcgcactct tcggttgtta acaacgcacc gaccgggaaa ccgccgccca gcgctttggc
     6061 ggtagttaac agatcaggtg tcacgccgta gtgcatatag gcatacagtt ccccggtgcg
     6121 cccgacgccg gtttgtactt catcaaaaat caacagcgca ttgtggcggt cacacaattc
     6181 acgcagacct tgtaaaaacg cgttgctggc tggcaccaca ccgccttccc cctggatggg
     6241 ttcgacaatc accgcacagg tggcgtcgtc aatcagcgcg ctggcagagt taatatcgtt
     6301 atatgcagca tgacggatat ctggtggcag tggcgcaaaa tcctgtgaat aggctggctg
     6361 cccacccgca ctgacagtaa acagcgtgcg accatgaaac gcatttttaa acgccacgat
     6421 accgctctta tggctgccgt agcggtcgtg agcgaattta cgcgccagtt ttagcgccgc
     6481 ttcgttggct tccgcaccgg agttacaaaa gaagacgcga tcggcaaacg tggcgtcgat
     6541 caattttttc gccagtcgca gtaccggctc gttggtgtaa ccgttgccgg tatgccagaa
     6601 cttactcgcc tgttcgttca gcgcttcacg cagttccgga tgcgcatggc ccagcgcgtt
     6661 caccgcaatg ccacccgcga agtcgatata ctctttcccc tgcagatccc acaagcgcga
     6721 accttcgcca cgtaccggta taaagggtgc cggagcgtaa acaggtatca tccattcatc
     6781 aaagttttca cgcgtaattg gctgagacat agcgacctct acagtaaata attcgctatc
     6841 tatatgttaa caataagtaa tgtttgcgct gtaaatgtag attgcagggt tcgtgccagc
     6901 cagtgataaa agtgcataaa cggcggaggc taactggaaa tcaaggagtt ataaccaaac
     6961 catatgcatt taaagtgcat ataaagtgaa tacgtttgcg atgtgggtga ataaaaagaa
     7021 taaaaaacgc aatgttatgc agaagtaaaa tataattctg gaattgtgat cattgacgaa
     7081 atttactgga aattactgcg ccattctgac gcagcgcgca ccaaaagcgg gcattttttg
     7141 cgccatcgtt gacatcatta acaaccatcg atcaaatcac ttaacaacag gcggtaagca
     7201 acgcgaaatt ctgctaccat ccacgcactc tttatctgaa taaatggcag cgactatgaa
     7261 atttgtctct tttaatatca acggcctgcg cgccagacct caccagcttg aagccatcgt
     7321 cgaaaagcac caaccggatg tgattggcct gcaggagaca aaagttcatg acgatatgtt
     7381 tccgctcgaa gaggtggcga agctcggcta caacgtgttt tatcacgggc agaaaggcca
     7441 ttatggcgtg gcgctgctga ccaaagagac gccgattgcc gtgcgtcgcg gctttcccgg
     7501 tgacgacgaa gaggcgcagc ggcggattat tatggcggaa atcccctcac tgctgggtaa
     7561 tgtcaccgtg atcaacggtt acttcccgca gggtgaaagc cgcgaccatc cgataaaatt
     7621 cccggcaaaa gcgcagtttt atcagaatct gcaaaactac ctggaaaccg aactcaaacg
     7681 tgataatccg gtactgatta tgggcgatat gaatatcagc cctacagatc tggatatcgg
     7741 cattggcgaa gaaaaccgta agcgctggct gcgtaccggt aaatgctctt tcctgccgga
     7801 agagcgcgaa tggatggaca ggctgatgag ctgggggttg gtcgatacct tccgccatgc
     7861 taatccgcaa acagcagatc gtttctcatg gtttgattac cgctcaaaag gttttgacga
     7921 taaccgtggt ctgcgcatcg acctgctgct cgccagccaa ccgctggcag aatgttgcgt
     7981 agaaaccggc atcgactatg aaattcgcag catggaaaaa ccgtccgatc acgcccccgt
     8041 ctgggcgacc ttccgccgct aatttagcag ctctcctggc tcaaactggg tcaggagaat
     8101 taaccttgag aaaaatcaac aaactgtcag taatgatttg ttgcctgccg tcctttgtta
     8161 taccgtcact gcgtttttag ttgtctgacc acttctctat tatcaagttt gatataggaa
     8221 actccacgat gaatgctgag cgtaaatttc ttttcgcctg tcttattttt gcgctggtca
     8281 tttacgctat ccacgctttc ggtttattcg atctgctcac cgatttaccc cacttacaga
     8341 cactcattcg ccagagcgga ttgttcggtt atagcctcta tattctgtta ttcatcattg
     8401 ccaccctctt tgtgttacca ggaagcatac tggtgatcgc tggaggaata gtttttggcc
     8461 cgctcttagg gacactactc tcattaattg ccgccacgct ggcctcctcg tgctcattcc
     8521 tgctggcgcg gtggctggga cgtgatttac tgctgaaata cgtgggccat agccatacct
     8581 tccaggccat tgaaaaaggc attgcgcgta acggtattga ttttcttatt ctgacccgct
     8641 taatcccgtt gtttccttac aatattcaaa attacgctta cggattaacc accatcgcct
     8701 tctggcctta tacccttatt tcggcactca cgaccctgcc cggtattgtt atttataccg
     8761 tgatggcaag cgatctcgcc aatgaaggca ttacgctgcg ctttatttta caactctgtc
     8821 tggcgggcct ggcgctgttt attctcgtcc agctcgcaaa actctacgcc cgacacaaac
     8881 atgtggatct gtctgcttcg cgccgcagcc cgcttactca cccaaaaaat gaaggataga
     8941 acgatgtcgc aacattattc agtgtcatgg aaaaaaggac tggctgcact ctgcttactg
     9001 gctgttgccg ggcttagcgg ctgcgatcaa aaagagaatg cggcggcaaa agtggaatac
     9061 gacggacttt cgaacagcca accgctgcgc gtcgatgcca ataaccatac ggtaaccatg
     9121 ttggtgcaaa ttaatggtcg tttcctcacc gacgacactc gtcacggtat tgtgtttaaa
     9181 gatggctcca acggacataa atcgctgttt atggcttatg cgaccccgaa agcattttat
     9241 gaagccctga aagaggcagg tggtacgccg ggcgaaaaca tgacgatgga taataaagaa
     9301 acgactcatg tcacaggcag caaactggat atttcggtca actggcaagg ggcggcaaaa
     9361 gcgtattcct tcgatgaagt gattgttgac agtaatggca agaaactgga catgcgcttt
     9421 ggcggtaatt taacggcagc agaagagaag aaaacgggtt gcctggtgtg tctggatagc
     9481 tgcccggtcg gcatcgtcag caatgcaaca tacacttatg gtgcggtgga aaaacgcggc
     9541 gaagtgaaat tcaaaggcaa tgcctcagtt ctcccggcgg ataacacgct ggcaacggtt
     9601 acctttaaaa tcaccgaata aagccaggat aaaggatgat gaagatgcaa tcgcgtaaaa
     9661 tctggtacta ccgtataacc ctcatcatcc tgttgttcgc tatgctgctc gcatgggcgc
     9721 tgcttcccgg cgtccatgag tttatcaatc gcagcgttgc ggcgtttgcc gccgtggacc
     9781 aacagggtat agaacgcttt attcagtctt acggcgcact ggcggcagtt gtctcgttct
     9841 tgttgatgat tttacaggcc attgctgcac cgctacctgc gtttttgatc acctttgcca
     9901 atgcgtcgct gtttggcgcg ttctggggcg gcttgctgtc gtggaccagt tcgatggccg
     9961 gcgcggcgct gtgctttttt atcgccagag tgatgggccg cgaagtggtg gaaaaattaa
    10021 ccggcaaaac cgtgcttgac agtatggacg gctttttcac tcgctacggc aaacacacca
    10081 tcctggtctg tcggttattg ccttttgtcc ctttcgatcc aatcagctat gctgccgatc
    10141 tgacttcaat acgttttcgc tcgtttttta tcgccaccgg gcttggtcaa ttaccgacga
    10201 ctattgttta ttcctgggcg ggcagcatgt taacaggcgg tactttctgg tttgtcaccg
    10261 gactgtttat tctgtttgcc ctgaccgtgg tgatttttat ggcgaagaaa atatggcttg
    10321 agcgccagaa gaggaatgac tgatgggttt accgccgctt agcaaaattc ctttcatttt
    10381 acgtccgcag gcgtggctgc atcgtcgcca ttacggcgag gtgctaagcc cgattcgctg
    10441 gtgggggcgg atcccgttta tcttttattt ggtgtcaatg tttgttgggt ggctggagcg
    10501 caaacgctca ccgctcgatc cggtagtgcg atcgcttgtc agcgcgcgca ttgcgcaaat
    10561 gtgcctgtgt gagttttgtg tggatatcac cagtatgaaa gtcgccgagc gtaccggcag
    10621 cagcgataaa ctgctggcag tggctgactg gcggcaaaac ccgctcttta gcgatgaaga
    10681 acggctggcg ctggagtacg ccgaagccgc aagcgtcaca ccgccaacag tcgatgatgc
    10741 cctgcgtacc cgactggctg cgcattttga cgctcaggcg ctcaccgaac tgacggcatt
    10801 gatcggcctg caaaacctgt cggctcgttt taattctgcc atggacattc ccgctcaggg
    10861 gctgtgccgt attccggaaa aacgttctta aggagagaag atgcgccatt gtgggtggtt
    10921 gctgggattg ttatcgctgt tttctttggc aacacatgcc agtgactggc aggaaattaa
    10981 aaatgaggcc aaagggcaaa ccgtctggtt taacgcctgg ggcggcgata ccgcaattaa
    11041 ccgctatctc gactgggtta gcggcgagat gaaaacccat tacgctataa acctgaagat
    11101 tgttcgtctc gcggatgccg cagacgcggt gaagcgcatt cagaccgaag ctgctgccgg
    11161 tcgtaaaacg ggcggctcgg tggatctgct ctgggtgaac ggcgaaaact tccgcacctt
    11221 aaaagaggcc aatttattac aaacgggctg ggcggagact ctgcccaact ggcgctatgt
    11281 cgacacacag ctgccggtgc gggaagattt ttcagtgccg acacaaggtg cggaatcgcc
    11341 ctggggcggc gcacaactga cgtttatcgc ccgccgcgat gttacgccac agccaccaca
    11401 aacgccgcaa gccttactgg agtttgctaa agccaatccc ggcacggtta cttatccgcg
    11461 cccaccggac tttaccggca cggcgtttct tgaacagttg ctgattatgc tgacgccaga
    11521 tcccgccgca ttaaaagaag cgccggacga tgcgactttc gcccgtgtca ctgcgccctt
    11581 gtggcaatat cttgatgcac tgcatccgta tttgtggcgc gaaggaaagg atttcccgcc
    11641 ttcgcccgcg cggatggatg ctctgctgaa agccggcaca ttgcgcctgt cgctgacctt
    11701 taaccccgcg catgcgcagc aaaaaatcgc cagcggcgat ttgccggcaa gcagttacag
    11761 ttttggcttt cgcgagggga tgattggcaa cgtgcatttc gtcaccattc cagccaacgc
    11821 gaatgccagt gctgcggcga aggtagttgc caatttcctg ctctcacccg atgcgcaact
    11881 gcgtaaagca gatcccgttg tctggggcga tccttctgtt ctcgatccgc aaaaactgcc
    11941 tgacgggcag cgcgaaatat tgcaatcaag aatgccgcag gatctgccgc cggtactggc
    12001 tgaaccgcac gcaggatggg tgaatgcact ggaacaagaa tggttacgcc gttacggtac
    12061 gcattaatct ttttgctgtg ggcgatggtg gcggtgattt atgcaccgct gatcccggca
    12121 gctctcacgc tgatatcgcc tgcgttgtcg ttgacacact ggcaggcgtt atttgccgat
    12181 ccacagttac cgcatgcatt actggcaacg ctggtatcga caaccatcgc ggcggtcggg
    12241 gcattgttga ttgccctgct ggtgattgtg gcgctatagc ctgggccgaa atggcagcgt
    12301 atgtgcgccc gtctgccgtg gctgctcgcc attccccatg tggcttttgc caccagcgcc
    12361 ctgctgctct ttgctgacgg agggctgcta tatgactatt tcccgtattt cactccgcca
    12421 atggaccgat ttggcatcgg tctgggcctc acccttgcag tgaaagaaag cgcatttctg
    12481 ctgtggatct tagcggcagt attgagcgaa aaacggctgt tgcagcaggt cattgtgctg
    12541 gattcgctgg gctacagccg ctggcaatgc ctgaactggc tgctgttgcc ctccgtcgcg
    12601 ccttcgctgg caatggcgat gctggcgatt gttgcctggt cgctttcggt cgtggatgtg
    12661 gcgattattc ttgggccagg taatccaccg acgctggcag taattagctg gcagtggtta
    12721 actcagggcg acgcggatca acaaacgaaa ggcgcactcg ccagtctgct gctgatgctg
    12781 ttactcgccg cctacgtttt gctgagctat ctgctatggc gcagctggcg gcgcactatt
    12841 ccccgcgtag atggcattcg caatgcctgc cacaccttta ttaccaggca ctacgctggc
    12901 gagtttttta cccttaaccg gtgtgctgtg tgtggttctg ctggcgatcc tcgcggatca
    12961 gtcgacgatc aatagtgaag cactcatcaa cagtctgaca atggggctgg tagcaacatt
    13021 catcgctttg ctcctgttac tgctgtggct ggaatggggg ccacagcgtc gccagttgtg
    13081 gctatggttg cccattctat tgcccgatct gccactggtg gcgggccagt acacgctggc
    13141 gctatggctg aacctggatg gaagctggac ggcggtggtc tgggggcatc tgctgtgggt
    13201 aatgccgtgg atgctgttta tcctgcaacc cgcctggcag cgcattgatt cacggttaat
    13261 tttgattgcg caaacactgg gctggtcgcg ggccaaaatc ttcttttacg tgaaatgccc
    13321 actcatgttg cgccctgtgc tgattgcctt cgcggtggga ttttcagtca gtattgcgca
    13381 gtatatgccg acgctgtggc tgggcgcggg gcgttttccg acgctcacca ctgaggcggt
    13441 ggcattaagc agcggcggca gcaacggtat tctcgccgcc caggctttat ggcaactgtt
    13501 gttaccgctt attatttttg ccctgaccgc gttagtcgca aaatgggtag gttatgtcag
    13561 acaaggactc cgctaatgct ctgcgtgaaa aatgtttcgc tacgtttacc agaaagccgc
    13621 ttgctgacaa acgttaactt tacggttgat aaaggtgaca tcgtcacgtt aatggggccg
    13681 tctggctgtg gaaaatccac tctgttttca tggatgattg gtgcactggc tggacagttt
    13741 tcttgtacag gtgagctatg gctcaatgag caacggattg acatgctacc caccgcacag
    13801 cgtcagattg gcattctttt ccaggatgca ctgttatttg accagttcag cgtcgggcaa
    13861 aatttactgc tggcgctact gtcgacgctc aaggggactg ccaggcgcaa tgccgtgaaa
    13921 gacgcgcttg atcgagcggg actggctgaa acttatcacc aggaccctgc gacattatca
    13981 ggcggtcagc gagcgcgggt ggccctgctg cgcgcccttc tcgctcagcc aaaagcgtta
    14041 ctcctggatg agccattcag ccgtcttgat gtggctctgc gcgataattt tcgccagtgg
    14101 gtgttcagcg aagttcgcga actggcgatc cccgtcgttc aggtaacgca cgatctccag
    14161 gatgttcctg ctgatagttc tgttctggat atggcgcagt ggtcagaaaa ttacaacaaa
    14221 ctgcgataac gcaaagtttt tctcaatgcg tcagttcaga atggcgcact caaaactaca
    14281 atgtcgggat tttcgatgaa acgtgtttct caaatgaccg cgctggcaat ggctttaggg
    14341 ctggcttgcg cttcttcgtg ggccgctgaa ctggcgaagc ctcttacact tgaccagctt
    14401 caacaacaaa atggcaaagc gatagatact cgccccagcg cgttttataa cggctggcca
    14461 caaaccttaa atggcccttc tggtcatgaa cctgccgcct taaacctctc tgccagctgg
    14521 ctcgacaaaa tgagcaccga acagctcaac gcgtggatca agcaacataa cctgaaagcc
    14581 gatgccccgg tggcgctgta cggtaatgac aaagatgtcg acgccgtcaa aacgcgactg
    14641 caaaaagcag gttttacgca tatctccatt ctgagtgacg cgctaagcga accttcccgt
    14701 ctacaaaaac tgccgcactt tgagcagctg gtttatccgc aatggctgca cgacctgcaa
    14761 caaggtaaag aggttacggc gaaacctgcc ggtgactgga aagtcattga agcggcctgg
    14821 ggcgctccta agctttacct tatcagccat attcccggtg ctgactacat cgataccaac
    14881 gaagtggaaa gcgaaccgct gtggaacaaa gtttctgatg aacaactaaa agcgatgctg
    14941 gcaaaacacg gcattcgcca tgacaccacg gtcattctgt acgggcgtga cgtatacgct
    15001 ggagcgcgtg tggcgcaaat tatgctctac gcaggcgtga aagatgtgcg tctgctggat
    15061 ggcggctggc aaacctggtc cgacgcggga ctgcctgttg agcgaggaac gccaccgaaa
    15121 gtgaaagcgg aaccggattt cggcgtgaag ctcccggcac aaccgcagtt gatgctcgat
    15181 atggaacagg cccgtggact gctgcatcgc caggatgcat cgctggtgag cattcgttcg
    15241 tggccagaat ttatcggtac gaccagcggt tacagctata ttaaaccaaa aggtgaaata
    15301 gccggagcac gttggggaca cgctggtagc gactcgacgc atatggaaga ttttcataac
    15361 ccggatggca ccatgcgcag cgccgatgat attaccgcta tgtggaaagc gtggaatatc
    15421 aaaccagagc agcaagtttc attctactgc ggcaccggct ggcgcgcgtc cgaaaccttt
    15481 atgtacgcac gcgccatggg ttggaagaat gtttccgtgt atgacggcgg ctggtacgaa
    15541 tggagcagcg atccaaaaaa tccggtagca accggtgaac gcggcccgga cagtagcaaa
    15601 taacattgca attactgacg ctggagcgac ttcagcgtca gatacccact ccacacccgc
    15661 gtaaaggtcg tcatccagca caacgcaccg aagatccacg caaaccacgc aaaccatgca
    15721 gggaataaac atcccagcac aaacagtaag atcgtttctg tgccttcggt taatcccccc
    15781 agatagtaaa acgacttatg tgcataaccg gggttatcaa tctgatgttt tgctgccagt
    15841 gcagcaaagg cgagaaaact gctgcccgtg ccgataaacg caaacaacaa ccagccgcct
    15901 gccagcgcat tttgctccgg tgcagcaaga ataaagccaa acggcaccag cgcgtaaaag
    15961 a
//
LOCUS       DADMKF010000091        15788 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.093, whole genome shotgun sequence.
ACCESSION   DADMKF010000091 DADMKF010000000
VERSION     DADMKF010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15788
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.093"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            163..765
                     /locus_tag="JNC24_002651"
     CDS             163..765
                     /locus_tag="JNC24_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAY9159361.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKNKRDATFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            complement(804..1229)
                     /gene="nudI"
                     /locus_tag="JNC24_002652"
     CDS             complement(804..1229)
                     /gene="nudI"
                     /locus_tag="JNC24_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HAY9159362.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVESGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
     gene            1508..2050
                     /locus_tag="JNC24_002653"
     CDS             1508..2050
                     /locus_tag="JNC24_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9159363.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIKSYEEANAGARYTIMHPVSIEAGYRYLNLSGK
                     DGNRDNAVADGPYVGVNASF"
     gene            2150..3352
                     /locus_tag="JNC24_002654"
     CDS             2150..3352
                     /locus_tag="JNC24_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HAY9159364.1"
                     /translation="MLKVEMLSTGDEVLHGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVRLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQSPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVVPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            3572..4354
                     /locus_tag="JNC24_002655"
     CDS             3572..4354
                     /locus_tag="JNC24_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HAY9159365.1"
                     /translation="MLESSKVPALTRAIDILNLIARIGPCSAATIIDTLGIPKSTAYL
                     LLNELRRQRFLSLDHQENFCLWTRLVELSGHALSKMDLRELARPRLTQLMDTTGLLCH
                     LGIIDNGSAYYILKVESSATISVRSHEGKSLSLYRSGIGKCLLAWQPAAVQQSIIEGL
                     VWEQATPTTITHPQQLHEELARIRRQGWSYDNGEDYPDVRCVAAPVFNANNELTAAIS
                     VVGTRLQINEEYRDYLAGKAIACARDISRLLGWKSPFDLQAS"
     gene            4287..5573
                     /gene="rhmD"
                     /locus_tag="JNC24_002656"
     CDS             4287..5573
                     /gene="rhmD"
                     /locus_tag="JNC24_002656"
                     /EC_number="4.2.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011310242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnonate dehydratase"
                     /protein_id="HAY9159366.1"
                     /translation="MPAPGIFHVCWDGKVPLTYRPHNNGEHHDPTKNQTGSRLVYWRC
                     DSRKRCWRGDYHDQGANHWIDDHIATPMSKYRDYEQSRQSFGINVLGTLIVEVEAENG
                     QTGFAVSTAGEMGCFIVEKHLNRFIEGKCVSDIKLIHDQMLNATLYYSGSGGLVMNTI
                     SCVDLALWDLFGKVVGLPVYKLLGGAVRDEIQFYATGARPDLAKEMGFIGGKMPTHWG
                     PHDGDAGIRKDAVMVADMREKCGEDFWLMLDCWMSQDVNYATKLAHACAPYNLKWIEE
                     CLPPQQYEGYRELKRNAPVGMMVTSGEHHGTLQSFRTLSETGIDIMQPDVGWCGGLTT
                     LVEIAAIAKSRGQLVVPHGSSVYSHHAVITFTNTPFSEFLMTSPDCSTMRPQFDPILL
                     NEPVPVNGRIHKSVLDKPGFGVELNRDCNLKRPYSH"
     gene            5630..6919
                     /locus_tag="JNC24_002657"
     CDS             5630..6919
                     /locus_tag="JNC24_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416749.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY9159367.1"
                     /translation="MSTALLDAVVKKNRVRLIPFMLALYVLAFLDRSNIGFAKQTYQI
                     DTGLSNEAYALGAGIFFVVYAFLGVPANLLMRKLGARTWIGTTTLLWGFLSAAMAWAD
                     TEAKFLIVRTLLGAAEAGFFPGMIYLTSQWFPQRNRASIMGLFYMGAPLALTLGSPLS
                     GALLEMHGFMGHPGWFWMFVIEGLLAVGAGVFTFFWLDDTPEQARFLSKQEKTLLINQ
                     LASEEQQKVTSRLSDALRNGRVWQLAIIYLTIQVAVYGLIFFLPTQVAALLGTKVGFT
                     ASVVTAIPWVAALFGTWLIPRYSDKTGERRNVAALTLLAAGIGIGLSGLLSPVMAIVA
                     LCVAAIGFIAVQPVFWTMPTQLLSGTALAAGIGFVNLFGAVGGFIAPILRVKAETLFA
                     SDAAGLLTLAAVAVIGSLIIFTLRVNRTVAQTDVAHH"
     gene            6937..7740
                     /gene="yfaU"
                     /locus_tag="JNC24_002658"
     CDS             6937..7740
                     /gene="yfaU"
                     /locus_tag="JNC24_002658"
                     /EC_number="4.1.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxy-L-rhamnonate aldolase"
                     /protein_id="HAY9159368.1"
                     /translation="MNALLTNPFKERLRKGEVQIGLWLSSTTSYMAEIAATSGYDWLL
                     IDGEHAPNTIQDLYHQLQAVAPYASQPVIRPVEGSKSLIKQVLDIGAQTLLIPMVDTA
                     DQARQVASATRYPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESKTALDN
                     LDEILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAAGFLAV
                     APDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRIKGSY"
     gene            complement(7781..8683)
                     /locus_tag="JNC24_002659"
     CDS             complement(7781..8683)
                     /locus_tag="JNC24_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001613492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HAY9159369.1"
                     /translation="MTESTTSSPHDAVFKTFMFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRLMRYATAA
                     MQRHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDRDLIGMVDRITTLLVKGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIEEISERSPLQKERLMTIAERLRQEGHQIGWQKGMHEQAIKIALRMLE
                     QGIDRDQVLAATQLSEADLAANNH"
     gene            complement(8876..10066)
                     /gene="glpC"
                     /locus_tag="JNC24_002660"
     CDS             complement(8876..10066)
                     /gene="glpC"
                     /locus_tag="JNC24_002660"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit C"
                     /protein_id="HAY9159370.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGIGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRKIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            complement(10063..11322)
                     /gene="glpB"
                     /locus_tag="JNC24_002661"
     CDS             complement(10063..11322)
                     /gene="glpB"
                     /locus_tag="JNC24_002661"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HAY9159371.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVTDIHSGLESLRQQAPAHPYSLLEPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRVTPLGTLRATWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDETLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            complement(11312..12940)
                     /gene="glpA"
                     /locus_tag="JNC24_002662"
     CDS             complement(11312..12940)
                     /gene="glpA"
                     /locus_tag="JNC24_002662"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HAY9159372.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            13213..14571
                     /gene="glpT"
                     /locus_tag="JNC24_002663"
     CDS             13213..14571
                     /gene="glpT"
                     /locus_tag="JNC24_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HAY9159373.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFLYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            14576..15652
                     /gene="glpQ"
                     /locus_tag="JNC24_002664"
     CDS             14576..15652
                     /gene="glpQ"
                     /locus_tag="JNC24_002664"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAY9159374.1"
                     /translation="MKLKLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRGHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMDLNLVQLIAYTDWNETQQKQPDGSWVNYSYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTTDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(15694..>15788)
                     /locus_tag="JNC24_002665"
     CDS             complement(15694..>15788)
                     /locus_tag="JNC24_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY9159375.1"
                     /translation="SLLAKEKPVEGSPLFLLQMCMEQLVAIGKI"
BASE COUNT         3661 a         3848 c         4254 g         4025 t
ORIGIN      
        1 tttatatttt ttcttattta ggctttgcat ttggcaaaat tttgaggcat tttgccgaca
       61 tcgtaggatt tttaatatta caccaactgc taattatcgc cagaaatgtc gctcaatttc
      121 acggtaattg tctggttgcg cttgtctata ggtggagttt acgtgttagc tttttgccgc
      181 tcatcattga agtcaaaaaa atatttcatc attttactgg cgctcgctgc aattgccgga
      241 ctgggtactc atgccgcctg gagtagcaat ggtttgccac gtatcgacaa taaaacattg
      301 gccagactgg cacagcagca cccggttgtc gttttgtttc gccatgctga acgttgcgat
      361 cgttcaacca atcaatgctt gtcagataaa acaggtatta cggttaaagg tacccaggat
      421 gcccgtgaac tgggcaacgc ttttagcgct gacatccctg atttcgatct ttattccagt
      481 aataccgtcc ggaccattca gtcggcgacc tggttttccg cgggtaaaaa actgacggta
      541 gataaacgac ttcttcagtg cggtaatgag atttatagtg caattaagga cttacaaagc
      601 aaagcgcctg ataaaaatat cgttattttc acccataatc attgcctgac atatattgct
      661 aaaaataagc gtgatgcgac atttaaacct gattatctgg atggtttagt catgcatgtg
      721 gaaaaaggca aagtttatct ggatggggaa ttcgttaacc actaaatttt caatctgaca
      781 gccagtgatg gctgtcatca ttgttaaaga agacctttca aacgtaacgt ttttcgggtg
      841 gcaacattta aatcataatg caccagatct tcaggtttta cccacgcgta gtcctgaaac
      901 tcttcgttta ttttcacttc tcggttggca gaaacgcagt caaaaatcag gtaaatcata
      961 taaatctctt ccttgcgacc atctgcatac gtcttggtgc gaatatcatc gctgaaggtc
     1021 cacggcgtga tttctgtcaa aagcagctgt tctcccagtt cttcgcgaat ttcgcggcgt
     1081 agtgcctctt caattcgttc gccagactcc acgccgccac ccgaaagcgc ccattgaccg
     1141 gggaaaacgc cgcgatcgtc ggccattttg cacagcaaat aagcaccatc attttgaatc
     1201 aaagggcata caatagtccg ttgtcgcaca ggggtattcc ttataaataa aaagaaacca
     1261 tgagaatacg gtaactcaga aatatgtgaa agtgacagta atctcttttg cgatgataac
     1321 tgcacttatt attatttttg tgtgtatcgg taaagtgtgt ttattttttc ggcgtttaat
     1381 tatttaagaa gtatttagtt acaataagtg actagatccc ttttttcgct cattgaatcc
     1441 tctgtgccag gcagtacaat cccgcagctt tgggaatttt gattaattgc ttgtgggaaa
     1501 ggggataatg aaaaaaattg cgctagcagg tctggccgga atgctgttgg tttctgcatc
     1561 ggtcaatgca atgagcatca gcggtcaggc gggtaaagaa tacaccaata ttggtgtcgg
     1621 ttttgggact gaatcgacgg gcctggcgtt aagcggtaac tggacacata acgacgacga
     1681 cggtgacgtc gctggcgtgg ggctggggtt gaatctgcct ctcgggccgt taatggcgac
     1741 cgttggcgga aaaggcgttt acaccaaccc gaattacggc gatgaaggtt atgccgcagc
     1801 ggtaggaggt ggtttgcagt ggaaaattgg caacagcttc cgtttgtttg gcgagtatta
     1861 ctactctccg gattcactct ccagcggtat taaaagttat gaggaagcga atgctggcgc
     1921 gcgttacacc attatgcatc cagtcagtat tgaggcgggt tatcgctacc tgaatctgtc
     1981 gggtaaagac ggtaaccgcg acaacgccgt ggctgacggc ccgtatgttg gggttaacgc
     2041 cagtttctga tcccttccgg cgcggcattc tgtcgcgcca ttcagtttct tactacgcct
     2101 tatctccctt tgcgttatag tgattcgata acaaaagcag gagataacca tgttaaaagt
     2161 ggaaatgtta tccaccgggg atgaagtgtt acacgggcaa atcgttgaca ctaacgctgc
     2221 ctggctggcc gattttttct ttcatcaggg gttgccatta tctcgccgca atacggtggg
     2281 ggataacctt gatgacttag tcaccattct tcgcgaacgt agtcagcacg ccgatgtgct
     2341 gatcgttaac ggcgggctgg gaccgaccag tgatgattta agtgcactcg ccgctgcgac
     2401 agcaaaaggt gaaggcctgg tgctgcatga agcctggctc aaagagatgg aacgctattt
     2461 ccacgaacgt ggacgagtaa tggcaccgag caaccgtaaa caagcggagc tgcctgccag
     2521 tgctgaattt atcaataacc cggtaggcac cgcctgtggt tttgccgtgc ggcttaatcg
     2581 ttgcctgatg ttctttactc ccggcgtacc gtcagaattt aaggtgatgg tcgagcacga
     2641 aatcctgccg cgcctgcgcg agcgtttttc tttaccacag tcgccggttt gtctgcgttt
     2701 aactaccttt ggtcgttcgg aaagcgatct ggcacaaagc ctggacactc tacaactgcc
     2761 gccgggcgta acaatgggct atcgctcctc aatgccgatc atcgaactga aactcaccgg
     2821 accggcaagc gagcaacagg cgatggaaaa actgtggctg gacgttaaac gtgttgccgg
     2881 acagagcgtg attttcgaag gcaccgaagg actgcccgcg cagatcagtc gcgaattgca
     2941 aaaccgccag ttcagcctga cgttgagcga gcaattcacc ggtggtttat tggctttgca
     3001 actttctcgc gcaggtgctc cattgctggc gtgtgaagtg gttccttcac aggaagaaac
     3061 cctggcgcaa actgcgcact ggattacaga acggcgggcc aaccattttg ccgggctggc
     3121 actggctgtt tcgggtttcg agaacgagca tctcaacttt gcgctagcca cgccagacgg
     3181 cactttcgct ctgcgtgtgc gtttcagcac tacacgctac agcctggcta tccgtcagga
     3241 agtgtgcgca atgatggcac tgaatatgct gcgccgttgg ttaaacggcc aggatatcgc
     3301 cagtgagcat ggctggattg aggttgttga gtccatgacc ttatctgtct gattccgcca
     3361 ctctgtgccg gtttactgca ccggcacatt ccccctccgg caaaacttgt ccaacctgtt
     3421 gtactcaata agaaaaaatc cgtgcactga ttcacaaatt tgtgcgtaat ttctccagtc
     3481 cgctggacga ggagcgggat ttgatgcgct tcacaatttt actccccctg agcaacgaga
     3541 ctgattccag tatctaagac tggagtccag tatgttggaa tcaagcaaag ttcccgccct
     3601 gacacgggct attgatattc tcaatttgat tgcccgcatt ggcccctgta gtgctgcgac
     3661 gatcattgac acattgggaa tccctaaaag cacggcctat ttgctgctta atgaactcag
     3721 gcgtcagcgt tttcttagcc ttgatcacca ggaaaatttt tgtttgtgga ccaggctggt
     3781 ggagctttct ggtcatgcgt taagcaagat ggatctccgg gaactggctc gaccgcgttt
     3841 gacgcaactg atggatacca ccgggctgtt atgtcacctg gggattattg ataacggcag
     3901 cgcttattac attttaaaag tggagtcatc agccactatc agcgtgcgtt cccatgaagg
     3961 aaaaagcctt tcgctttatc gctccggaat aggtaaatgc ctgcttgcct ggcaacctgc
     4021 agcggtacag caaagtatta tcgaagggct ggtctgggag caggcgacac ccacaacgat
     4081 tactcatcca cagcaacttc acgaagaact ggcgcgtatt cgccgtcagg gctggagcta
     4141 tgacaacggc gaagattacc ccgatgtccg ttgtgtcgct gcgccggtgt ttaacgccaa
     4201 taacgagctt accgctgcga tctcggtggt cggtacccgc ttacaaatca atgaagaata
     4261 ccgtgattat ctggcgggca aggccattgc ctgcgccagg gatatttcac gtctgttggg
     4321 atggaaaagt ccctttgact tacaggcctc ataacaatgg agaacatcat gaccctacca
     4381 aaaatcaaac aggttcgcgc ctggtttact ggcggtgcga cagcagaaaa aggtgctggc
     4441 gcggtgatta tcacgaccag ggggcgaatc actggattga cgatcatatt gccaccccga
     4501 tgagtaaata ccgcgattac gagcagtcac gccagtcatt tggcattaac gttcttggca
     4561 cgttgattgt tgaagtcgaa gcagaaaacg gccagaccgg attcgctgtt tcgacagccg
     4621 gtgaaatggg ctgttttatt gtcgaaaaac atcttaaccg tttcattgag ggtaaatgtg
     4681 tcagtgatat caaactgatc catgatcaaa tgctcaatgc caccctgtat tactccggct
     4741 ctggtggcct ggtgatgaac acgatttcct gtgtcgatct ggctctgtgg gatctgttcg
     4801 gcaaagtggt cgggcttccg gtttataaac ttttaggcgg cgcagttcgt gatgagattc
     4861 agttctacgc cacaggcgcg cgtccggatc tggcaaaaga gatgggcttt atcggtggca
     4921 aaatgccgac gcactggggg ccacatgatg gcgatgcggg gatccgcaaa gatgccgtta
     4981 tggtcgcgga tatgcgtgaa aaatgcggtg aggatttctg gttaatgctc gactgctgga
     5041 tgagtcagga cgtgaactat gccaccaaac tggcccacgc ttgcgcgccc tataacctga
     5101 aatggatcga agagtgcctg ccgccacagc agtatgaagg ttatcgcgaa ctgaaacgca
     5161 acgcgccagt cggaatgatg gtcaccagcg gtgagcacca cggcacactg caatcttttc
     5221 gtacgctttc agaaaccggt atcgacatta tgcagccgga tgttggctgg tgcggtggtt
     5281 taaccacgct ggtggaaatt gccgcaatcg ccaaatccag ggggcaactg gtggtgccgc
     5341 acggttcgtc tgtttactct caccatgcgg tgatcacctt caccaatacg ccattcagcg
     5401 aattcctgat gaccagcccg gattgttcaa cgatgcgtcc gcagtttgac ccgattctgc
     5461 tcaatgagcc ggttccggtg aatggtcgta ttcataaatc agtgcttgat aaacccggtt
     5521 ttggcgtcga actcaatcgt gactgcaatt tgaaacgccc ctacagccac taatcactcc
     5581 gggcgttgct gtgacaacgg taacgcccac atattctaag gagcttttta tgagcaccgc
     5641 tttgcttgac gccgtggtga agaaaaaccg tgtgcgttta attccgttta tgttggcgct
     5701 gtatgtgctg gcgtttctcg accgttcgaa tatcggtttt gccaaacaga cctaccagat
     5761 tgataccggg ttgagtaatg aagcttatgc gctgggagca ggcattttct ttgtggtata
     5821 tgcgtttctg ggtgttccgg cgaatctttt gatgcgcaaa ctgggggcca gaacctggat
     5881 tggtacgaca acactgctgt ggggatttct ttcggcagcc atggcatggg ccgatactga
     5941 agcgaaattt ctgatagttc gcactctgct tggtgctgcg gaggccggat ttttccctgg
     6001 tatgatttat ctcacctcgc aatggtttcc gcagcgtaat cgcgccagca ttatggggct
     6061 gttctatatg ggcgcaccgc tggcgttaac actgggatca ccgctttccg gcgcgctgtt
     6121 ggagatgcat ggatttatgg ggcatcccgg ctggttctgg atgtttgtga ttgaaggatt
     6181 gttggcagtc ggtgctgggg tattcacatt cttttggctt gatgacacac cggagcaggc
     6241 acgttttctg agtaaacaag aaaaaacgtt gcttatcaat caactggcaa gtgaagaaca
     6301 acagaaagtg acttctcggc tgagcgatgc gctgcgtaat ggccgagtct ggcaactagc
     6361 gattatctac ctgaccattc aggtggcggt ttacggatta attttcttcc tgccgaccca
     6421 ggttgcggca ttgctgggaa caaaagtggg ctttacagcg tcggtggtca ccgccattcc
     6481 gtgggttgcg gccttgtttg ggacctggct tattccgcgc tattccgata aaaccggcga
     6541 acggcgtaat gtcgcagcgc tgacattact ggcggcgggc attggtattg gtctgtccgg
     6601 gctgctttct ccagtaatgg cgatcgtagc gctgtgtgtt gcagctatcg ggtttattgc
     6661 cgtgcagccg gtgttctgga cgatgccgac acaactgctt tccggtacgg cgctggctgc
     6721 ggggattggt tttgtaaacc tgtttggtgc agtgggcggg tttattgccc cgatcctgcg
     6781 cgtgaaagca gaaacgttat ttgccagcga tgcggcagga ttactgacac tggcagcggt
     6841 ggcggtcatc ggttcgctga ttattttcac tctgcgtgta aatcgtactg ttgcgcagac
     6901 cgacgtggca catcattaaa taggttaagg aacacgatga acgcattatt aaccaatccc
     6961 tttaaagaac gtttacgcaa gggcgaagtg caaattggtc tgtggttaag ctcgactact
     7021 tcttatatgg cagaaattgc cgctacttcc ggttatgact ggttgctgat tgacggggag
     7081 cacgcgccaa acaccattca ggatctttat caccagctac aggcggtagc gccctatgcc
     7141 agccaacccg tgatccgtcc ggtggaaggc agtaaatcgc tgattaaaca agttctggat
     7201 attggcgcgc aaactctatt gatcccgatg gtcgatactg ccgatcaggc tcgtcaggtg
     7261 gcgtctgcca cgcgctatcc tccctacggt gagcgtggtg ttggggccag tgtggcacgg
     7321 gctgcgcgct ggggacgcat tgagaattac atggcgcaag tcaacgattc gctttgtctg
     7381 ttagtgcagg tggaaagtaa aacggcactg gataacctgg acgagatcct cgacgtcgaa
     7441 gggattgatg gcgtgtttat tggaccagcg gatctttctg cgtcgttggg ctacccggat
     7501 aacgccgggc acccggaagt gcagcgaatt attgaaacca gcattcggcg gatccgtgct
     7561 gcgggtaaag cggctggttt tctggctgtg gctcctgata tggcgcagca atgcctggcg
     7621 tggggagcga actttgtcgc tgttggcgtt gacacgatgc tctacagcga tgccctggat
     7681 caacgactgg cgatgtttaa atcaggcaaa aatgggccac gcataaaagg tagttattga
     7741 tatcaaaggc ccatggggat cggctgtggg cctgtgttaa ttagtggtta ttcgctgcca
     7801 gatcggcttc gcttagctgg gtggccgcga gcacctggtc acgatcaatg ccctgttcca
     7861 gcatgcgcaa agcaatttta atggcttgtt catgcatacc tttctgccag ccaatttggt
     7921 gtccttcctg ccgtagccgc tcagcaatag tcatcaatct ctccttttgt agtggtgaac
     7981 gttcggaaat ctcctcaata aaacgggtga aacgggaggt atcgccgcat tgcagcagat
     8041 aattaaacag tgtttgtagc tggctgtcat tagtgaagcc tttaaccaaa agcgtggtga
     8101 tcctgtcgac cataccgatt aaatcgcggt cgcgaatatg cttttgaatc agttcaagca
     8161 gagctatacg ccgatgttgc atgatctcat cgtcaggtac gatggtgata tccaccaacg
     8221 gaaaagcttc ggtgtacaac tgccgggcaa gttgcggatc gtcaaactca tccagccagt
     8281 taagtgagta cgggtagggc gatgtttcgc catgataaaa cagcaatggc accaccagcg
     8341 gaactctgtc atagccttta tccaggtgac gctgcatggc ggcagtggca tagcgcatta
     8401 gccgaaaagc catattcttt tctgcagagc tttgatgttc aatcacgcag tagatatagc
     8461 cgtcaccgtc gctggtttcc acggaccaca aaacatccga gtagtaagcg cgtaaacttt
     8521 tttcaataaa actagtgggt tccaggcgta aggtttgcag gttgcaaagc ttgcgcagtg
     8581 gttctggcaa atgtatttcg agaaaatccc gtgcggtttc gggtgtgaac ataaaggttt
     8641 taaataccgc atcatgcggg gaggaggttg ttgattcggt catggttgtc cgtcacctga
     8701 tatttatgat gacgcgacat tacggttatc ccaggccttt gacatctgcc agtttcagcc
     8761 tttgcgagac gttttcagag gaattttcac tgcgttgcaa cgcatacatt gggcacggaa
     8821 tcgtgtacgc aaaaatgccc ggtaaccaca tgcttaccgg gcatcagaaa ggagtttaag
     8881 ccagcgcctg ggccagtagc gtaatcggat gttcgcagcg aagacttgtg gacatctcaa
     8941 tctgccattt acaggtttcg cagtcggtga ccaccagatc tgcgccgctt tcttctatct
     9001 ggcggaacag tggtgcgccg atggcttgtg aggtggggta gttctctttt ttgaaaccgt
     9061 aagtacccgc aataccgcag cactgggaat ccagcaccgt taactcaagc cccgggattt
     9121 tacgcaacag ctccagggtg tagagcgtcc agcccatttt ttccatatgg cacggagtgt
     9181 gataaaccac tttcagcggc agcggtttca gcggtaacgt tttgccttcg tccagcttgc
     9241 gccacagcca gcgggttgcc agttcgatat gatcgcgcaa gcctttgttg tcgacattca
     9301 gcacttccgg gtattcgtcg cgcagggcaa atgtacaggt tgaggaggtg gcaatcaccg
     9361 gaatgccttt tactcccaca gcttcgcgga tcgactctac attcgtaatt gcctgtttgc
     9421 gtgctttatc ggtaaagccg ttggcgatta gcggtacgcc gcagcatttt tctttgctga
     9481 gcagttgtac accgataccc attgcgttga gcactttaat taaatcttta cctaactgcg
     9541 gatggttgta gttaacgaag cagccgtgaa agaaagcgac ctggtcttta tattgtgctt
     9601 gctgagccgc tatgctgcga taccagcgac ggaacgtgcc gaaggagtat ttcggtagcg
     9661 tgcgacgatg atcgattttt aacgccgcat caagcagctg ccgcaccggt ttcagcgagg
     9721 tagcggtgtt gacgattggt gcgaacggcg tggaaacgct acccatcagg tcggtatgac
     9781 tcaacacaaa attacgcagc gacgggcgcg tggtgtcata ttttgcccgc gcgcgctgga
     9841 taatatcgcc aatcttcaca tcggacgggc aggcgacttc acaacgtttg cagttgatgc
     9901 aatatttcag cgcctcgtca tacagtgcgc catctttcaa acgcagacgc tcgccatccg
     9961 gcccggcttg ttttggccct ggataaccgg gattcacccg gctcaccggg caggcggtgg
    10021 tgcagacggt gcacttaatg cagttttcga agctggtgtc gttcattgtt ggcctcctgc
    10081 gcgttgggca atctgttgag cggcatgtaa agcactgacg gcacaaacac cgccgccgca
    10141 tccttgggcg atgggatcaa atccgcccag caccgaaccg atggcaaaca ggttctcaat
    10201 ggtttgccct gcctgcgacg ggcgtagcgt ctcatcagtg gttacaccga actgctgcca
    10261 cggttgcggc gcaaaaaaat cgcccttata ccattcaccc cgcgtggcgg tttgtagcac
    10321 atcaaggccg agaatcggct cgcgaatgcc gttacgttcc gctaccagtc cgccactaaa
    10381 gaaactgccg ctggcgagaa ccgcgaaacg tggacgtagc ggaatatcgg cgtgattgcg
    10441 ggtccagatt tcattcacta cgccattttt acaggtcact tttttcactt catcgcccgg
    10501 catccacacg ccaccctggc gcacaaactg gcgctgtaac tggttttgca gacgaatgcc
    10561 cagcacggaa ggcggcagcg ttggcaaaag catcagtgaa caaggtagtt tttcattcaa
    10621 ccaacgccac agtttgtcat cggccaggcc gaagcaggcg ggcatcagga tcatttcgca
    10681 ggtattggcg acaggaataa gcgcatcaag taacagcggc cagttttctt cattatcaag
    10741 gaaacgggcg atattcaccg cgcgaaattc ggtggcgtta ttgcgcagga catccagttc
    10801 cggcagctct atttctgcgg tttcaaccga aagatccagt tcgcgcaacg aagccgccgc
    10861 aagatgcgcc tggaaatcca tcagcccgct tatccccact acacatatct tcttcgcggg
    10921 tagcggccag acgggaactt ctggcgaact taaccaggtt gcacgcaaag tgccgagcgg
    10981 ggtaacgcgc tgatgggcca gttccacgct gccctgcaat tgcgcaccgc tttcagcgat
    11041 caatgcctgc gcctggcaag cgagatcgag cacgcgttgt ggttcgagaa gactgtaagg
    11101 atgggctggt gcctgctgac gcaaagattc cagtccactg tgaatgtctg tcaccggttg
    11161 accatcaggc agatggctca gcaaatccag cgaaccggat gagaaatgca gcgcgctttg
    11221 accacgcgtg acaatggcac agcgcaggcc gtgtttttgc agttgcaggc cacagagtaa
    11281 tccggcgagg ccgccgccca taatgacagt atcaaagcgc atctttctgc tccttctcca
    11341 gaccacacag tccctgataa acccagcggg taaattcgct ttcgcgcagt gcatctcccc
    11401 aggcgatggg ttgcacgcct ttccagcgtt cgttaaggaa ggtggaaagt tgctcgatag
    11461 attgcgcgga cgtcgtgacg ttaaaacgtt gcagcagtcc ggcagcgcgg caggcgcaga
    11521 gttcgccctg gcaggtaccc atccccacac gggtacgacg gcgtaaatcc agcaggctat
    11581 taacgtttaa attttctacc gcgtactgca cttcacccgc tgttaccgct tcgcactcac
    11641 ataccaggct acggtgcaga cgaccttcgc tcagccaggc aggcgtgcga tcgccatgac
    11701 gataaaccgc agaaccgcgc agcggggcag gcagggagat gactttacgc aaggtaactt
    11761 cagcgggttc ctgtgaacca ggaagtgcca gatcggcggt cgtacagggg cgcgtgttgc
    11821 ccagtttgcg gcacacggcg tcggtagccc attcagccat cagccgatag gtcatcagtt
    11881 tgccaccggt gatggtgata aatccgtcca gaccatcgcg ttcagcatgg tcgagcagca
    11941 cgatgccacg gctgacgtta cgtccgctcg ggtcgtcatc gctggcaacc agcgggcgca
    12001 cgccagaata ggcccgcaaa atgcgcgttt tcgccatcac gggggccagt ttttcccctt
    12061 cacgcagcag aatatcaacc tcttctgccg tcactcgatt atcgtcaatc tcgttgtaat
    12121 caatacgtaa agaggtggtg ccaatcagcg aaatggtatc gccaggcacc agaatatcgg
    12181 cgtcggaagg tttacggcag cggttgatca catgctggtt aatgcggtga tccatgatca
    12241 gcagcgatcc tttcgccggg aacatgcgaa tgcgcagatc ggcatattcg gcaatgtgtt
    12301 gcccccagat cccggcggca ttaaccacga caggcgcatg aagggcctga gtttcgccgg
    12361 tgagatggtt acgtacacga acaccgcaca ccgtcgcgcc ttcacgaatc agtcccgtga
    12421 cttcatgagc ggtaaggata acggcaccgt gttctttggc atccagcatg tttgctgcgg
    12481 tcagacgaaa tggatcaacg gtgccatccg gaactttcac cgcgccaatc aatgccgggt
    12541 taacggcagg ttcgataatg cgcgcttgct gcgggtctat agcttctgcg ctgatccctg
    12601 cttcttcgca ggcgcgaata aaagtggcct ggaaggagag gtcatcttcc ggcagggtaa
    12661 taaacaggcc gttggttggt tcaacgcagt gacgtgcaat gcgtttcagg atctggtttt
    12721 cactaatgca ttcgcgggcc gattccgcat cggttaccgc atagcgcgca ccgctgtgta
    12781 gcaggccgtg gttacgcccg gtggcaccgg ttgcgatgtc gtggcgctca accaaaatca
    12841 cgcgcagccc gcgcagggca cagtcgcggg caatcccggc tcccgttgcg ccgccgccaa
    12901 tgataatcac gtcacttgat tgcgagtcgc gagttttcat tgttatccct ctgaagttcg
    12961 ttttttacca tttagccata gtaaaaacat gaattgtttg atttcgcgca tattcgctca
    13021 tgattcgaaa gtgaagcgtg atttcatgcg tcattttgaa cattttgtaa atcttattta
    13081 ataatgtgtg cggcaattca catttaattt atgaatgttt tcttaacatc gcggcaactc
    13141 aagaaacggc aggttctctc actgaatcag gctgttaatc ataaataaga ccacgggcca
    13201 cggaggctat caatgttgag tatttttaaa ccagcgccac acaaagcgcg cttacctgcc
    13261 gcggagatcg atccgactta tcgtcgattg cgctggcaaa ttttcctggg gatattcttt
    13321 ggctatgcgg cttactattt ggttcgtaag aactttgcgc ttgctatgcc ttatctggtt
    13381 gagcagggat tctcacgcgg tgatttaggt tttgcccttt cggggatctc gattgcttat
    13441 ggattttcga aattcatcat gggttcggta tcggatcgct cgaatccgcg cgttttcctg
    13501 cccgcaggtt tgattctggc ggcggcagtg atgttgttta tgggctttgt gccatgggcg
    13561 acgtcgagca ttgcggtgat gtttgtactg ttgttcctct gcggttggtt ccaggggatg
    13621 gggtggccgc cgtgtggtcg tactatggtg cactggtggt cgcagaaaga acgtggcggc
    13681 attgtgtcag tgtggaactg tgcgcacaac gtcggtggtg gtattccgcc gctgctgttc
    13741 ctgctgggga tggcctggtt caatgactgg catgcggcgc tctatatgcc tgctttctgc
    13801 gccattctgg tggcattatt cgcctttgcg atgatgcgcg ataccccgca atcctgtggc
    13861 ttgccgccga tcgaagagta caaaaatgat tatccggacg actataacga aaaagcggaa
    13921 caggagctga cggcgaagca aatcttcatg cagtacgtac tgccgaacaa actgctgtgg
    13981 tatatcgcca tcgccaacgt gttcgtttat ctgctgcgtt acggcatcct cgactggtca
    14041 ccgacttatc tgaaagaggt taagcatttc gcgctggata aatcctcctg ggcctacttc
    14101 ctttatgaat atgcaggtat tccgggcact ctgctgtgcg gctggatgtc ggataaagtc
    14161 ttccgtggca accgtggggc aaccggcgtt ttctttatga cactggtgac catcgcgact
    14221 atcgtttact ggatgaaccc ggcaggtaac ccaaccgtcg atatgatttg tatgattgtt
    14281 atcggcttcc tgatctacgg tcctgtgatg ctgatcggtc tgcatgcgct ggaactggca
    14341 ccgaaaaaag cggcaggtac ggcagcgggc tttaccgggc tgtttggtta cctgggcggt
    14401 tcggtggcgg cgagcgcgat tgttggctac accgtggact tcttcggctg ggatggcggc
    14461 tttatggtaa tgattggcgg cagcattctg gcggttatct tgttgattgt tgtgatgatt
    14521 ggcgaaaaac gtcgccatga acaattactg caaaaacgca acggaggcta atggcatgaa
    14581 attgaagctg aaaaacctta gcatggcgat catgatgagc actatagtca tgggaagcag
    14641 tgcaatggcg gcggacagca acgaaaaaat agtcatcgcc catcgcggtg ccagtggata
    14701 tttgccggag catacgctgc cagcaaaagc gatggcgtat gcgcagggag cggattatct
    14761 ggaacaggat ttggtgatga ccaaagacga ccatctggtt gttctgcatg accattatct
    14821 cgatcgtgtt actgatgttg ccgatcgttt cccggatcgg gcgcgcaaag acggtcgtta
    14881 ctacgcgata gatttcacgc tggatgaaat taagtcgctg aaatttaccg aaggtttcga
    14941 tattgaaaac ggtaaaaaag tacagactta tccggggcgt ttcccaatgg gtaagtccga
    15001 cttccggggg cacacctttg aagaagagat tgaatttgtt caggggttaa atcactctac
    15061 cgggaaaaat atcggtatct atccagaaat caaagcgccg tggttccatc atcaggaagg
    15121 gaaggatatt gcggcaaaaa cgctggaagt gctgaagaaa tatggttaca ccggtaagga
    15181 cgataaagtt tatttgcaat gttttgatgc tgatgagctg aagcgtatta agaatgagct
    15241 ggaacctaaa atgggcatgg atctcaatct ggtacagctg attgcctata ccgactggaa
    15301 tgaaacgcag cagaaacagc cggacggaag ctgggttaat tacagctatg actggatgtt
    15361 taagccaggt gccatgaagc aagtcgcgga atatgccgac ggtatcgggc cggattacca
    15421 tatgttgatt gaggaaacat cgcagccggg taatatcaaa ctcactggca tggtgcaaga
    15481 tgctcagcag aataaactgg tagtgcatcc ttataccgtg cggtcggata aactgcctga
    15541 atacactact gatgtgaatc agttatatga tgctctgtat aacaaagcgg gtgtaaatgg
    15601 gctgtttact gatttccctg ataaggcagt aaaattcctt aataaagagt aatgctactg
    15661 caggatggcg ttcatgcgcc atcctgtctc atatcaaatt tttccaatcg ccactaattg
    15721 ttccatgcac atttgtagta aaaacagtgg actgccttct accggctttt ctttagccaa
    15781 caacgaaa
//
LOCUS       DADMKF010000092        15583 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.094, whole genome shotgun sequence.
ACCESSION   DADMKF010000092 DADMKF010000000
VERSION     DADMKF010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15583
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.094"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            <1..2067
                     /locus_tag="JNC24_002666"
     CDS             <1..2067
                     /locus_tag="JNC24_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HAY9159376.1"
                     /translation="SGYVPPENWERGINAFYTSYYVSQYYSDYKASGNSKSTYVRFNS
                     GLNLLGWQLHSDASFSKTNNNPGVWKSNTLYLERGFAQLLGTLRVGDMYTSSDIFDSV
                     RFRGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVTIEQNGFVVYQKEVPPGPFAITD
                     LQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQPGVSKYDFAAGRSHIEGASKHS
                     DFVQAGYQYGFNNLLTLYGGSMVANNYYAFTLGTGWNTRIGAISVDATKSHSKQDNGD
                     VFDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRTFNDHVWANNKDNYRRDENDVYD
                     IADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLWRDYWGRSGSSKDYQLSYSNNLR
                     RISYTLAASQAYDENHHEEKRFNIFISIPFDWGDDVSTPRRQIYMSNSTTFDDQGFAS
                     NNTGLSGTVGSRDQFNYGVNLSHQHQGNETTAGANLTWNAPVATVNGSYSQSSTYRQA
                     GASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDAYVNGQKYRTTNRNGVVVYDGMT
                     PYRENHLMLDVSQSDSEAELRGNRKIAAPYRGAVVLVNFDTDQRKPWFIKALRADGQP
                     LTFGYEVNDIHGHNIGVVGQGSQLFIRTNEIPPSVNVAIDKQQGLSCTITFGKEIDES
                     RNYICQ"
     gene            2083..3117
                     /locus_tag="JNC24_002667"
     CDS             2083..3117
                     /locus_tag="JNC24_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
                     /protein_id="HAY9159377.1"
                     /translation="MEIRIMLFILMMMVMPVSYAACYSELSVQHNLVVQGDFALTQTQ
                     IATYEHNFNDSSCVSTNTITPMSSSDIIVGLYNDTIKLNLHFEWTNKNNITLSNNQTS
                     FTSGYSVTVTPAASNAKVNVSAGGGGSVMINGVATLSSASSSTRGSAAVQFLLCLLGG
                     KSWDACVNSYRNALAQNAGVYSFNLTLSYNPITTTCKPDDLLITLDSIPVSQLPATGN
                     KATINSKQGDIILRCKNLLGQQNQTSRKMQVYLSSSDLLAGSNTILKGAENNGVGFML
                     ESNGSPVTFLNITDNSKGYTNLKEIAAKSKLTDTTVSIPITASYYVYDTNKVKSGALE
                     ATALINVKYD"
     gene            complement(3199..3537)
                     /gene="rcnB"
                     /locus_tag="JNC24_002668"
     CDS             complement(3199..3537)
                     /gene="rcnB"
                     /locus_tag="JNC24_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HAY9159378.1"
                     /translation="MTIKNKMLLGVLLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(3756..4580)
                     /locus_tag="JNC24_002669"
     CDS             complement(3756..4580)
                     /locus_tag="JNC24_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
                     /protein_id="HAY9159379.1"
                     /translation="MTEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIA
                     IKGTIKQAVMLGLAATISHTAVVWLIAFGGMVISKRFTAQSAEPWLQLISAVIIIGTA
                     FWMFWRTWRGERNWLENMHEYDYEHHHHDHEDHHDHGHHHHHEHGEYQDAHARAHAND
                     IKRRFDGREVTNWQILLFGLTGGLIPCPAAITVLLICIQLKALTLGATLVVSFSIGLA
                     LTLVTVGVGAAISVQQVAKRWSGFNTLAKRAPYFSSLLIGLVGVYMGVHGFMGIMR"
     gene            4701..4973
                     /gene="rcnR"
                     /locus_tag="JNC24_002670"
                     /pseudo
     CDS             4701..4973
                     /gene="rcnR"
                     /locus_tag="JNC24_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
     regulatory      5065..5161
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5196..5984
                     /gene="thiM"
                     /locus_tag="JNC24_002671"
     CDS             5196..5984
                     /gene="thiM"
                     /locus_tag="JNC24_002671"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HAY9159380.1"
                     /translation="MQVDLLGSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTPWTLDPVA
                     VGALDYRRHFCHELLSFKPAAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRAVGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENVASACHWMKQAGERAVARSEGLGSFVPHFLDALWQLTQEVQA"
     gene            5981..6781
                     /gene="thiD"
                     /locus_tag="JNC24_002672"
     CDS             5981..6781
                     /gene="thiD"
                     /locus_tag="JNC24_002672"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HAY9159381.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKSWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            6846..7664
                     /locus_tag="JNC24_002673"
     CDS             6846..7664
                     /locus_tag="JNC24_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 25 family protein"
                     /protein_id="HAY9159382.1"
                     /translation="MQLRITSRKKLTALLCALGLISIVAIYPRQTVNFFYSTAVQITD
                     YIHFYGYRPVKSFAIRIPASYTIHGIDVSRWQERIDWQRVAKMRDNGIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVAAPVQVRLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKSTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFNGTVEELQGFVDGIKETP"
     gene            7716..8462
                     /locus_tag="JNC24_002674"
     CDS             7716..8462
                     /locus_tag="JNC24_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAY9159383.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(8436..9401)
                     /locus_tag="JNC24_002675"
     CDS             complement(8436..9401)
                     /locus_tag="JNC24_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HAY9159384.1"
                     /translation="MSGARLHTLLPELTTRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     GDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGSLLYFSGYQLASPCGEL
                     LVEWLEELQDVTPFIDFGPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGEQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(9398..10402)
                     /locus_tag="JNC24_002676"
     CDS             complement(9398..10402)
                     /locus_tag="JNC24_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HAY9159385.1"
                     /translation="MKTERILGALYGQALGDAMGMPSELWPRSRVKAHFGWIDRFLPG
                     PKENNAACYFNRAEFTDDTSMALCLADALLEREGKIDPDLIGRNILDWALRFDAFNKN
                     VLGPTSKIALNAIRDGKPVAELENNGVTNGAAMRVSPLGCLLPARDVDSFIDDVALAS
                     SPTHKSDLAVAGAVVIAWAISRAIDGESWSAIVDSLPSIARHAQQKRITTFSASLAAR
                     LEIALKIVRNADGTESASEQLYQVVGAGTSTIESVPCAIALVELAQTDPNRCAVLCAN
                     LGGDTDTIGAMATAICGALHGVNAIDPALKAELDAVNQLDFNRYATALAKYRQQREAV
                     "
     gene            complement(10399..11676)
                     /locus_tag="JNC24_002677"
     CDS             complement(10399..11676)
                     /locus_tag="JNC24_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY9159386.1"
                     /translation="MKTTAKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFAGALLMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIGWIASGLACGFLPQILGYADISPT
                     NIPLLITAGSSALLGVFAFFLPDTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLVTAAIRYGFFIYGSTDEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYQEPVNGLTFNWSGMWTFGAVMIAII
                     AVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            11932..12984
                     /gene="fbaB"
                     /locus_tag="JNC24_002678"
     CDS             11932..12984
                     /gene="fbaB"
                     /locus_tag="JNC24_002678"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HAY9159387.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLASVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGTTIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            13303..14157
                     /gene="gatY"
                     /locus_tag="JNC24_002679"
     CDS             13303..14157
                     /gene="gatY"
                     /locus_tag="JNC24_002679"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HAY9159388.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTYAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEADVFYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPALDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFSQALKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            14186..15443
                     /gene="gatZ"
                     /locus_tag="JNC24_002680"
                     /pseudo
     CDS             14186..15443
                     /gene="gatZ"
                     /locus_tag="JNC24_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416598.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
     gene            15453..>15583
                     /locus_tag="JNC24_002681"
     CDS             15453..>15583
                     /locus_tag="JNC24_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="phosphoenolpyruvate-dependent sugar
                     phosphotransferase system; catalyzes the phosphorylation
                     of incoming sugar substrates concomitant with their
                     translocation across the cell membrane; IIB is
                     phosphorylated by IIA and then transfers the phosphoryl
                     group to the sugar; IIC forms the translocation channel;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HAY9159389.1"
                     /translation="MTNLFVRSGISFVDRSEVLTHIGNEMLAKGVVHDTWPQALIAR"
BASE COUNT         4088 a         3728 c         3989 g         3778 t
ORIGIN      
        1 agtggctatg ttccaccgga aaactgggag cggggtatta atgcgtttta tacctcttat
       61 tatgtgagtc agtattacag tgactataaa gcgtcgggta atagtaagag tacatatgta
      121 cgttttaaca gcgggttaaa tttactgggg tggcaactgc attctgatgc cagtttcagt
      181 aaaacaaata acaatccagg ggtgtggaaa agcaataccc tgtatctgga acgtggattt
      241 gcccaacttc tcggcacgct tcgcgtgggt gatatgtaca catcaagcga tatttttgat
      301 tctgttcgct tcagaggtgt gcggttgttt cgtgatatgc agatgttgcc taactcgaaa
      361 caaaatttta cgccacgggt gcaggggatt gctcagagta acgcgctggt aactattgaa
      421 cagaatggtt ttgtggttta tcagaaagag gttcctcctg gcccgtttgc gattacagat
      481 ttgcagttgg ccggtggtgg agcagatctt gatgtcagcg tgaaagaggc ggacggttcg
      541 gtaaccacct atctggtgcc ttatgcagcg gtgccaaata tgctgcaacc cggcgtgtcg
      601 aaatatgatt ttgcggcggg tcgtagccat attgaagggg cgagcaaaca tagtgatttt
      661 gtccaggcgg gttatcagta tggttttaat aacttattga cgttgtatgg tggctcgatg
      721 gttgcgaata attattacgc gttcactttg gggactggct ggaatacacg cattggtgcc
      781 atttccgtcg atgccacgaa gtcgcatagt aaacaagaca acggcgatgt gtttgacggg
      841 caaagttatc aaattgccta caacaaattt gtgagccaaa cgtcgacgcg ttttggtctg
      901 gcggcctggc gttattcgtc gcgtgattac cggacattta acgatcacgt ttgggcaaac
      961 aataaagata attatcgccg tgatgaaaac gatgtctatg acattgccga ttattaccag
     1021 aatgattttg gtcgcaaaaa tagcttttcc gccaatatga gccagtcatt gccagaaggt
     1081 tgggggtctg tgtcattaag tacgttatgg cgagattact gggggcgtag cggcagtagt
     1141 aaggattatc agttgagtta ttccaacaac ctgcgacgga taagctatac cctcgcggca
     1201 agccaggctt atgacgagaa tcatcatgaa gagaaacgtt ttaatatttt tatatcgatt
     1261 ccctttgatt ggggtgatga cgtttcgacg cctcgtcggc aaatatatat gtctaactca
     1321 acgacgtttg atgatcaggg gtttgcctca aataatacgg gattatcagg aacagtaggg
     1381 agtcgggatc agttcaatta tggtgtcaac ctgagtcatc aacatcaggg aaatgaaacg
     1441 acagctggag cgaatttaac ctggaacgcg ccggtggcaa cagtgaatgg cagttatagt
     1501 cagtcgagta cttatcgaca ggctggagcc agtgtttcag ggggcattgt cgcctggtcg
     1561 ggtggcgtta atctggcgaa ccgtctttcc gaaacgtttg ctgtgatgaa tgcgccagga
     1621 attaaagatg cttatgtcaa tgggcaaaaa tatcgcacaa caaaccgtaa tggagtggtg
     1681 gtatacgacg gaatgacacc ttatcgggaa aatcacctga tgctggatgt gtcgcaaagc
     1741 gatagcgaag cagaattacg tggcaaccgg aaaattgccg caccttatcg cggcgcggtt
     1801 gtactggtta attttgatac cgatcagcgc aagccctggt ttataaaagc gttaagagcg
     1861 gatgggcaac cattaacgtt tggttatgaa gtcaatgata tccacggtca taatattggc
     1921 gttgtcggcc agggaagcca gttatttatt cgcaccaatg aaataccgcc atcggttaat
     1981 gtagcaattg ataagcaaca aggactttca tgcacaatca ccttcggtaa agagattgat
     2041 gaaagtagaa attatatttg ccagtaatag tcaggtggtt ctatggaaat tcgcataatg
     2101 ctatttatat taatgatgat ggttatgcct gtgagctatg cggcatgtta tagtgagtta
     2161 tctgttcagc acaatttggt tgttcagggg gattttgcac ttactcaaac acaaatagcg
     2221 acatatgagc ataattttaa tgattcgtca tgcgtgagta caaatactat cacccctatg
     2281 agttcgtcgg atattattgt tgggctttat aacgatacca taaaattaaa tttacatttt
     2341 gaatggacca ataaaaacaa catcacgttg tcaaataatc agaccagttt caccagtggt
     2401 tattcagtta cggtgacacc tgcggccagt aatgcaaaag tgaatgtttc tgcggggggc
     2461 ggcggttcag tgatgattaa tggtgttgcg acattatcca gtgcttcatc atcgacacgc
     2521 gggagtgccg cagtacaatt tctactgtgt ttattaggtg gcaagtcatg ggatgcatgt
     2581 gtaaatagct acagaaatgc attggcacaa aatgcaggtg tctattcctt taatctgaca
     2641 ttgtcataca acccgataac cacaacctgc aaaccggacg atttattaat tactttagac
     2701 agtattcccg tttcacaatt accagccaca ggtaacaaag caacaataaa tagtaaacaa
     2761 ggggatatta ttctgcgttg taaaaattta ttaggtcaac aaaatcaaac atcacggaaa
     2821 atgcaggtgt atttatcaag ttctgatttg ttagccggca gtaacacgat cctgaaaggt
     2881 gcagaaaata atggagtcgg atttatgctt gaaagcaatg gttctccagt cacattttta
     2941 aatatcactg ataacagcaa aggatacaca aatttaaaag aaattgcggc aaagtcaaaa
     3001 cttacagata caacggtttc aattccgata actgccagtt actacgtcta cgatacaaac
     3061 aaagttaaat ctggcgcact ggaggcaacc gcattaatca acgtgaaata cgactaataa
     3121 caagcaagac gagcaagtgg ctaataataa aaaataacaa ggtgtctgca tttccccctc
     3181 atgatgaggg ggcttttttt agcgatgata aaaaatctca ccgtcgtagg ctttaatgat
     3241 tttaccgtcg gtgtcgctga tcaacacgta cgcgccaccc atataggtcc agtgcgtccc
     3301 ggcatcaggc gcgggcaggt tacgtaactg ccactgctta atgttgtact catcggtgcg
     3361 gtacatctct gggacggtat cacctggttt gaaatgctta aagtcagcaa tgaaactact
     3421 taactcatac ttagaaattc ccgaggtatt ggtcgaaccc gcggtggctg gtgcggccca
     3481 ggcggcactg gtaaccagca aaagcacacc cagcaacatc ttatttttaa tggtcataaa
     3541 ctctccactt tggggatctg taatatcagt cgcatatctt tcctgccata caatataact
     3601 gacaagcgat attttgttag tgatcccatg atttcgtaaa aagtgtaaat gaaaggatgc
     3661 cctaaaagca tccttagcga gcagagagtc agcggaaata gctgatttaa gaatcatcct
     3721 gggctgaaga taacatgcaa gcctaaagga ttgagttatc gcattatgcc catgaagcca
     3781 tgtacgccca tatacacacc gactaagcca atcaacagac tggaaaaata gggggcgcgt
     3841 ttagcgagag tgttaaatcc gctccagcgt tttgcgacct gctgaacgct gattgctgcg
     3901 ccaacgccta cggtgacaag cgttaacgcc aggccaatgc tgaaactgac gaccagtgtt
     3961 gcgcccagtg tcagggcttt caactgaatg catatcaaca gcacggtaat tgctgccggg
     4021 caggggataa ggccaccggt taagccaaat agcaaaattt gccagttggt gacctctcta
     4081 ccatcaaagc gtcgtttaat gtcattggca tgggctcgtg catgggcatc ctgatactcg
     4141 ccatgttcgt gatggtgatg atgtccatgg tcgtggtgat cttcgtgatc gtgatgatga
     4201 tgctcataat catactcgtg catattctcc agccagttgc gttcgccgcg ccaggtacgc
     4261 cagaacatcc agaacgcggt gccaataatg atcactgcgg aaatcagctg gagccacggt
     4321 tctgctgatt gagcagtaaa gcgcttgctg atcaccatcc cgccaaaggc aattaaccag
     4381 accactgcgg tatgcgaaat agttgctgcc agtccgagca tcaccgcttg tttaatggtg
     4441 cctttgatgg cgatgataaa cgccgccatc atcgtttttg agtgccctgg ttccaggcca
     4501 tgaagcgcac caagtaagat ggcgctgggg atgaagaacc aggcgtttcc ttgctgaaga
     4561 agagttgtaa attcggtcat gataataatt cttagtatta attcggcaat ctgattctac
     4621 tccccccagt acctgatact accccccagt agattaatag tgctatgata ttttcatatt
     4681 cttgttaacc aggtgttgcc atgtctcata caatccgtga taaacagaaa ctgaaagcgc
     4741 gtgccagtaa gattcagggc taggtcgtgg cgctcaagaa aatgctcgac gagccgcatg
     4801 aatgcgctgc agttttacaa cagattactg ctatccgtgg cgcggtaaac ggtctgatgc
     4861 gggaagtgat taaaggtcat ctgacggaac acatcgttca ccagggggat gagctaaaac
     4921 gtgaagaaga tctggatgtc gttctgaagg tgctggattc atatatcaaa taatttatta
     4981 acgcgattgt aaaactgccg tttttcctcg tttacaacgc gtgcgctgga cattaccatc
     5041 ctcctctgcg atttatcatc gcaaccaaac gactcggggt gcccttctgc gtgaaggctg
     5101 agaaataccc gtatcacctg atctggataa tgccagcgta gggaagtcac ggaccaccag
     5161 gtcattgctt cttcacgtta tggcaggagc aaactatgca agtcgacctg ctgggttcag
     5221 cgcaatctgc gcacgcgtta cacctttttc accaacattc ccctcttgtg cactgcatga
     5281 ccaatgatgt ggtgcaaacc tttaccgcta ataccttgct ggcgctcggt gcatcgccag
     5341 cgatggttat cgaaaccgaa gaggccagtc agtttgcggc tatcgccagt gccttgttga
     5401 ttaacgttgg cacactgacg cagccacgcg ctcaggcgat gcgtgctgcc gttgagcaag
     5461 caaaaagctc tcaaacaccc tggacgcttg atccagtagc ggttggtgcg ctcgattatc
     5521 gccgccattt ttgtcatgaa cttttatctt ttaaaccggc agcgatacgt ggtaatgctt
     5581 cggaaatcat ggcattagct ggcattgcta atggtggacg gggagtggat accactgacg
     5641 ccgcagctaa cgcgataccc gctgcacaaa cactggcacg ggaaactggc gcaatcgtcg
     5701 tggtcactgg cgaggtggat tatgttaccg atggccatcg cgccgttggc attcacggtg
     5761 gcgatccgtt aatgaccaaa gtggtaggaa ctggctgtgc attatcggcg gttgtcgctg
     5821 cctgctgtgc gttaccaggc gatacgctgg aaaatgtcgc atctgcctgt cactggatga
     5881 aacaagccgg agagcgcgca gtcgccagaa gcgaggggct aggcagtttt gttccacatt
     5941 tccttgatgc gctctggcaa ttgacgcagg aggtgcaggc atgaaacgaa ttaacgctct
     6001 gacgattgcc ggtactgatc cgagtggtgg tgcggggatt caggccgatc ttaaaacctt
     6061 ctcggcactt ggcgcttatg gttgctcagt tattactgca ctggtggcgc aaaatacccg
     6121 tggcgtacag tcggtgtatc gcattgagcc tgattttgtc gccgcccagc tcgattcggt
     6181 gttcagcgat gtgcgaatcg ataccactaa aatcggtatg ttggcggaaa ccgatattgt
     6241 tgaagcggtg gcagaacggt tgcaacgtta tcagatccaa aacgtggtac tcgacaccgt
     6301 tatgctggca aaaagcggcg acccactgct ttcaccttcg gcagttgcca cgctacgcag
     6361 tcgattattg ccacaggttt cattaataac gccaaacttg cccgaagcag ccgccttgct
     6421 cgacgcgcca cacgcgcgca ccgaacagga aatgctggaa caagggcgat cgctgttggc
     6481 gatgggctgt ggcgcagtgc taatgaaagg tggtcatctg gatgatgagc aaagcccgga
     6541 ctggctgttt acccgcgagg gtgaacaacg gtttaccgca ccgcgcatta tgaccaaaaa
     6601 cacccacggt actggttgta cactctctgc ggcgttggct gcactacgcc cgcgccatac
     6661 aaactgggct gacaccgtac aggaggcaaa aagctggctt tcatcggcgt tagcccaggc
     6721 cgacacgctg gaagttggtc acggtattgg tccggttcac cacttccacg cctggtggtg
     6781 agcaccgtac tatagctgct ttggttattg tcagcctttg gcactgacat tcagggatga
     6841 aaccaatgca gctaagaatt accagtcgaa aaaaactcac cgctctatta tgcgctcttg
     6901 ggctaatttc catcgttgca atttatccgc gtcagacggt gaattttttc tattcgacag
     6961 cagttcagat tacagactac atccacttct acggttatcg cccggttaaa tctttcgcta
     7021 ttcgtattcc tgccagttac accattcacg gaatagatgt ttcgcgctgg caggagcgga
     7081 tcgactggca gcgtgtggca aaaatgcgcg ataacggtat tcgcttacaa tttgccttta
     7141 ttaaggcgac ggaaggcgaa aagctggtgg acccctattt ttcgcgtaac tggcaactaa
     7201 gccgcgaaaa tggcttgctg cgcggggcat atcattattt ctccccgtcg gtagcagcac
     7261 cggttcaggt gcgattattt ctgcaaacgg tagatttctc acaaggcgat ttccctgcgg
     7321 tactggacgt agaagagcga ggaaaattat cggcaaaaga attacgcaag agggtaagtc
     7381 agtggctaaa aatggtcgaa aaaagtacgg ggaaaaagcc gattatttac tcaggagccg
     7441 ttttttatca cacgaatctg gcgggctatt tcaatgaata tccgtggtgg gtggctcact
     7501 actatcaacg tcgtccggac aatgacggca tggcctggcg cttctggcag cattccgacc
     7561 gtggacaggt agatggcatc aatggtccgg tggattttaa tgtgtttaat ggcacggtgg
     7621 aggagttgca gggattcgtt gatgggatta aagaaacgcc ttaaataaga agcagtgata
     7681 tactcccacc agactccctg gcaggaaaaa gacaaatgga acaagcgcat acccagctga
     7741 ttgcccaact gaacgaacgt attttagcgg cggataacac gccgctttat attaagtttg
     7801 ccgaaacggt aaaaaatgcc gtgcgcagcg gggtgctgga gcatggcaat attttgcccg
     7861 gtgagcgtga tttaagtcag ttaaccggcg tttcgcgcat tacggtgcgc aaggcgatgc
     7921 aggcgctgga agaagagggc gtggtgacgc gttcgcgcgg ttacggcacg cagatcaaca
     7981 acatcttcga atactcgttg aaagaagcgc gaggtttttc tcagcaggtg gtgttgcgcg
     8041 ggaaaaagcc cgatacgcta tgggttaaca agcgtgtcgt gaaatgtccc gaagaagtcg
     8101 cgcagcagct ggcggtcgaa gcaggaagtg atgtcttttt gcttaagcgt attcgctatg
     8161 tcgatgaaga agcggtatcg attgaggaat cgtgggttcc ggcgcattta attcatgatg
     8221 ttgatgccat cgggatttca ctttatgact atttccgcag ccagcacatt tacccacaac
     8281 gtacgcgttc ccgcgttagc gcccggatgc cggatgccga gtttcagtca catattcagt
     8341 tagatagcaa aataccggtg ctggtgatca agcaagtggc gcttgaccaa cagcaacggc
     8401 ctattgagta cagcattagc cactgtcgca gcgatctata cgtttttgtg tgcgaggagt
     8461 agttcctcgc gcgttggcgc acaatcaccg ccccgatgcc cgacaaccca cgacgccact
     8521 gcattgccca gtaatacggc atccgccagt ggcagaccag aggccagccc ggcaagtacg
     8581 ccacctgcat gactgtcgcc cgccccaatg gtgtctacaa cttgcgttgg aaatgccgga
     8641 atgcagccag aagcgtcgtt gctgaaatac catgcgcctt ctttatcgag acgaacgatc
     8701 aacggcgcgg caaatttctc ctgccattgc tcgccaagtg ttgttatctc tgcggataaa
     8761 gcaaaacgtt cggcggcaat ctcagcctct tgacgattga gcgacactaa aggtcgacag
     8821 gccatgatcc gcgccagtaa tgcatccggg atatcgccaa tacgtgggcc aaaatcgata
     8881 aacggcgtca cgtcttgcag ctcttccagc cattccacta acaattcgcc gcagggcgag
     8941 gccagttgat aaccggaaaa atagagcagg ctgccaggcg caacggttaa tcgcgccagc
     9001 cactggcgat tccactgatt ttccacaccg ctgaatgaca taaaagtgcg ttcgccatcc
     9061 ggctcaacca gcgccagaca ccagccgtta tcaccttcgg cgttatcgat caggctgatt
     9121 aagccctctt ttgccatccg gttgcgaata atctccgccc acacgccctg accgagcggc
     9181 aaggcattac ccgcttcgat gccgaggcgc tttaacgcca cggcaatatt cagtgcgcag
     9241 ccgccaacgt taacgctctg ctgtttcagt tcgatatcgc acccacgcca ggggagggca
     9301 taagcgtcgg cgatcacgtc aatgaccgcc gcgccgacga ccatcaccgg ctgacgcgtg
     9361 gttaattcag gcagcagcgt gtgtaatcga gcgccgctca taccgcctcc cgttgttgac
     9421 gatatttcgc cagcgctgtg gcatagcggt tgaaatcaag ctgatttacc gcatccagtt
     9481 ccgcctttaa cgcaggatcg atagcgttaa cgccatgcaa cgcgccgcaa attgccgtcg
     9541 ccatagcacc gatggtgtct gtgtcgccgc caaggttagc gcacaggacg gcgcagcgat
     9601 tcgggtcggt ttgtgctagt tcaaccagcg caatggcgca cggaacggac tcaatagtgc
     9661 tggtacctgc gccaacgacc tggtaaagct gttcgctggc ggattcggtg ccgtcggcat
     9721 tgcgcacaat tttcagcgca atctccagcc gcgccgccag tgaggcgctg aacgtagtga
     9781 tgcgtttttg ctgtgcatgt cgcgcaattg aaggcaggga atcaacaatc gctgaccagc
     9841 tttctccgtc aatggcacga gaaatcgccc atgcgatgac taccgcgcct gcaaccgcca
     9901 gatcggattt atgggtcggg ctggacgcca gcgctacatc atcaataaag gaatcaacat
     9961 cacgcgccgg aagcaaacaa cctaatggcg agacgcgcat cgctgcgccg ttggtcacgc
    10021 cgttgttttc cagttcagca acgggtttac cgtcgcgaat ggcgttaagc gcaatcttcg
    10081 aggtcggacc tagtacgttt ttgttaaagg cgtcgaaacg cagcgcccag tcgagaatat
    10141 tacgcccaat cagatccgga tcgatcttgc cttcacgttc cagtaacgca tccgccagac
    10201 acagcgccat cgaggtatcg tcggtgaatt cggcgcggtt aaaataacag gccgcgttat
    10261 tctcctttgg tccaggaaga aaacggtcaa tccagccaaa gtgtgcttta acgcggctgc
    10321 gtggccaaag ctcggagggc atccccatcg catcccctaa cgcctgccca taaagagcac
    10381 cgagaatacg ttctgttttc atttaacttc cccttgtgtc aacgcaatat cgcgatcatc
    10441 gaccttgata gccgtaattt cgttgtcgga ttcgcggaaa aaaatcatga acagcacggc
    10501 gataatggca atcatcaccg cgccgaaagt ccacatcccg gaccagttga aagtcagtcc
    10561 gtttaccggt tcctgataag cgaacatctt ttccatcatc acaccgccaa gacgatagcc
    10621 gagcaaactg ccgaagccct ggcagcagag cgtgatcagc ccctgcgcag cggtacgcat
    10681 atgcacgggg gcttttttat cgacatagat gtaagcggta acgtagtaaa aatcgtaact
    10741 tacgccgtga agcaaaatac cgaggaacag taacgcgtag gtgaaatatt catccgtact
    10801 accgtaaata aagaagccat agcggatcgc agcggtgacc agaccaagca ataatacctt
    10861 tttgatacca aagcgtttag tgaaaaacgg caatgccagc ataaagaaga tttcagagaa
    10921 ctggccgagc gtcatccagc cggtggcgtt tttcatgcca acttcggtca gataaccgtt
    10981 ggcaaagatg taatagaacg ccagtggcat cgcaaacagg aatgaacaga agaaaaagac
    11041 gaggaagttt ttatcgcgca gcaggatcag cgcatccagg ccgagcatga ctttaatatc
    11101 cattttgccg gtgctttttg gtggcgtgtc gggcaggaaa aacgcaaaca caccgagcag
    11161 agcagaactt ccggcggtaa tcagcagcgg gatgttagtc ggtgagatat cggcataccc
    11221 cagtatttgc ggcaagaaac cacatgccag accggaggca atccagccga tagtgcccat
    11281 cacacgaatg cgcgggaaat cacgctccac atccggcacg ttggcaaaag cgatgctgtt
    11341 agtcagcgca atggtcggca tataggttag agagtaagcc agcagtaacg gaaagaaccc
    11401 ggcaaaagtg gtctgttgcg cagcgaaata catcagcagc gcgcctgcga acatcaatac
    11461 cgccagcact ttttgcgccg agaaaaagcg gtcagtgatg gagccaacca gaattggcga
    11521 caggatcgcc gcaatggcgg tacaggcata cgaccagcca atttctccgg cactaaaacc
    11581 gcttttactt aaccacaacc acaatggcac aaaccacgcg ccccagataa accattcaac
    11641 aaacatcatg aacgacagct ttgctgttgt tttcatcttt taattccttc atgacaggta
    11701 atgtaaggta cgcgatgaca ataccactta acaagccttt taaatacctt tgcggtgaaa
    11761 acttgaccgc gctaacattt tttctgatga atcgagccaa cagaaaacgc tgaaaaaaca
    11821 tccaaaagat ggaaaaactc gcccgtaacg accaggatta tgattgcccg caaaaggcgg
    11881 gccaggacaa tccatagccg atatccaatc ggaatttacg ggagcatagt aatgacagat
    11941 attgcgcagt tgcttggcaa agacgccgac aaccttttac agcaccgttg tatgacaatt
    12001 ccttctgacc agctttatct ccccggacat gactacgtag accgcgtaat gattgacaat
    12061 aatcgcccgc cagcggtgtt acgtaatatg cagacgttgt acaacaccgg gcgtctggct
    12121 ggcacaggat atctttctat tctgccggtt gaccagggcg ttgagcactc tgccggagct
    12181 tcatttgctg ctaacccgct ctactttgac ccgaaaaaca ttgttgaact ggcgatcgaa
    12241 gcgggctgta actgtgtggc gtcaacttac ggcgtgttgg cgtcggtatc gcggcgctat
    12301 gcgcatcgca ttccatttct cgtcaaactt aatcacaacg agacgctaag ttacccgaat
    12361 acctacgatc aaacgctgta tgccagcgtg gagcaggcct tcaacatggg cgcggtggcg
    12421 gttggtacga ctatctattt tggctcggaa gagtcacgtc gccagattga agaaatttct
    12481 gcggcctttg aacgcgcgca cgagctgggc atggtgaccg tgctgtgggc ctatttgcga
    12541 aactccgcct ttaagaaaga tggcgttgat taccatgttt ccgccgacct gaccggtcag
    12601 gcaaaccatc tggcggcaac catcggtgca gatatcgtca aacaaaaaat ggcggaaaat
    12661 aacggcggct ataaagcaat taattacggt tacaccgacg atcgtgttta tagcaaattg
    12721 accagcgaaa acccgattga tctggtgcgt tatcagttag ctaactgcta tatgggccgg
    12781 gctgggttga taaactccgg cggtgctgcg ggcggtgaaa ctgacctcag tgatgcagtg
    12841 cgtactgcgg ttatcaacaa acgcgctggc ggaatggggc tgattcttgg acgtaaagcg
    12901 ttcaagaaat cgatggctga cggcgtgaaa ctgattaacg ccgtgcagga cgtttatctc
    12961 gatagcaaaa ttactattgc ctgatacgcc tcatacgaac agcaccaccc cgtaggacgg
    13021 ataaggcgtt tacactgcat ccggcaacct atgcctgatg cgacgcggaa gcgtcttatc
    13081 atgcctacat agcactgcca cgtatgtcgg aaaaggcgtt tacaccgcat ccggcataaa
    13141 aacacgcgca ctttgctacg gcttccctat cgggaggccg tttttttgcc tttcactcct
    13201 cgaacagttt tcatattgtc gttttgtgat cgttatctcg acatttaaaa acaaatcatt
    13261 tcattatatt ttgaaatcga aaacaagcaa caggatatga aaatgtacgt ggtatcgaca
    13321 aagcagatgc tgaacaacgc acagcgcggc ggttatgcgg ttccggcatt caatattcac
    13381 aatctcgaaa cgatgcaagt ggtggtggaa accgctgcca acctgcatgc gccggtcatc
    13441 atcgccggaa cgcctggcac attcacttat gctggtacag aaaatctgtt ggcgctggtc
    13501 agcgcgatgg cgaagcaata tcaccatcca ctggcaattc atctcgacca tcacacgaaa
    13561 tttgacgata tcgctcagaa ggttcgttct ggcgtgcgct cagtcatgat tgacgcctcg
    13621 catttgcctt ttgcgcaaaa tatttcacgg gtcaaagagg tggtggattt ttgccatcgc
    13681 tttgatgtca gcgtcgaagc ggagctgggg caacttggcg gccaggaaga tgatgtgcaa
    13741 gtcaatgaag ccgatgtttt ttataccaac cccgctcagg cgcgtgaatt tgccgaggca
    13801 accggaattg attccctggc ggtcgccatc ggcacggctc atgggatgta tgccagcgca
    13861 ccggcgcttg atttttctcg actggagaac attcgccagt gggtgaactt accgctggtg
    13921 ctgcatggcg cgtcagggtt atcgactaag gatattcagc aaaccatcaa actggggata
    13981 tgcaaaatca acgttgcaac ggagctgaaa aatgccttct cgcaggcgtt aaaaaattac
    14041 ctgaccgagc accctgaagc gaccgatccc cgggattatt tgcagtcggc taaatccgca
    14101 atgcgcgatg tggtgagcaa agtgattgcc gattgtggct gcgagggcag ggcataacgc
    14161 acctgccatt taacaaggaa aaaacatgaa aacgttaatt gcccggcata aagctggtga
    14221 acatatcggc atatgttcag tctgttctgc ccatccgttg gttatcgaag cggcgctggc
    14281 atttgatcgc aacagcacgc gcaaagtgct gattgaagca acgtcaaacc aggtcaatca
    14341 atttggcggt tataccggaa tgacaccggc agactttcgc gaatttgttt ttacgattgc
    14401 cgataaagtc ggatttgcac gcgaacgcat tattctcggc ggcgatcatc tggggccaaa
    14461 ctgctggcag caagaaaatg cggatgcggc gatggaaaaa tccgtcgagc tggtaaaagc
    14521 atatgttcgt gctggcttca gtaaaattca tcttgatgcg tcaatgtcct gcgcggggga
    14581 tcccataccg ttagcaccag aaacggttgc ggaacgagct gctttgcttt gctgcggaaa
    14641 gtgtggcgac agattgccag cgtgagcaac tgagctatgt cattggcact gaagttccgg
    14701 ttccgggcgg tgaggccagc gccattcagt cagtacacat cacccatgtt gaagatgccg
    14761 ccaatacttt acgtacgcat caaaaggcct ttattgcccg tgggctgaca gaggcgttaa
    14821 cacgcgtgat tgccatcgtg gtgcagccgg gcgtggaatt tgatcacagc aatattatcc
    14881 attatcagcc gcaggaagcg cagccgctgg cgcaatggat agaaaacacc cgaatggttt
    14941 atgaagcaca ttctaccgat taccagaccc ggacggctta ttgggaatta gtccgcgatc
    15001 actttgcaat attgaaagtc ggtcccgcat taacctttgc tttacgcgag gcgatatttg
    15061 cactggcaca aattgagcag gaacttatcg cccctgaaaa tcgcagcggt tgcctggcgg
    15121 taattgaaga agtgatgctc gacgaaccgc aatactggaa aaaatattat cgtacgggtt
    15181 ttaacgattc attactggat attcgttaca gcctgtcgga tcgtattcgt tattactggc
    15241 cgcatagtcg gattaaaaat agcgtcgaaa cgatgatggt gaatcttgaa ggcgtggaca
    15301 tcccactggg catgattagt cagtatcttc ccaaacaatt tgaacgcatt cagtccgggg
    15361 aattatcagc aataccgcat cagctgatta tggataaaat ttatgatgtt ttgcgcgcct
    15421 atcgctacgg ctgtgcggaa taaggacggt atatgactaa cctgtttgtt cgtagcggaa
    15481 tttcttttgt cgatcgtagc gaagttttaa cccatatcgg taatgagatg ctcgccaaag
    15541 gtgtggttca tgatacctgg ccacaggcat taattgccag aga
//
LOCUS       DADMKF010000093        15445 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.095, whole genome shotgun sequence.
ACCESSION   DADMKF010000093 DADMKF010000000
VERSION     DADMKF010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15445
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.095"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(97..1209)
                     /gene="ydiK"
                     /locus_tag="JNC24_002682"
     CDS             complement(97..1209)
                     /gene="ydiK"
                     /locus_tag="JNC24_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HAY9159390.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGSIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            1598..4654
                     /locus_tag="JNC24_002683"
     CDS             1598..4654
                     /locus_tag="JNC24_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HAY9159391.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDTATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKTNTTIGRIYNTVY
                     QRCRLQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITPLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIASQQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLEALNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESHPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHENSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            4651..5061
                     /gene="menI"
                     /locus_tag="JNC24_002684"
     CDS             4651..5061
                     /gene="menI"
                     /locus_tag="JNC24_002684"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HAY9159392.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGFLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGIC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            5161..5349
                     /locus_tag="JNC24_002685"
     CDS             5161..5349
                     /locus_tag="JNC24_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9159393.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSTELKEEIYFAWRLGVH"
     gene            5897..6265
                     /gene="sufA"
                     /locus_tag="JNC24_002686"
     CDS             5897..6265
                     /gene="sufA"
                     /locus_tag="JNC24_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HAY9159394.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQAMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFGV"
     gene            6274..7761
                     /gene="sufB"
                     /locus_tag="JNC24_002687"
     CDS             6274..7761
                     /gene="sufB"
                     /locus_tag="JNC24_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HAY9159395.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            7771..8517
                     /gene="sufC"
                     /locus_tag="JNC24_002688"
     CDS             7771..8517
                     /gene="sufC"
                     /locus_tag="JNC24_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HAY9159396.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            8492..9763
                     /gene="sufD"
                     /locus_tag="JNC24_002689"
     CDS             8492..9763
                     /gene="sufD"
                     /locus_tag="JNC24_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HAY9159397.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGDISPQQRDALALTLDAVRLVFVDGRYVPALSDAIE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPLSHHFAHNDLLLAEDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKMAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            9760..10980
                     /gene="sufS"
                     /locus_tag="JNC24_002690"
     CDS             9760..10980
                     /gene="sufS"
                     /locus_tag="JNC24_002690"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HAY9159398.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEIITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNNIAEYEQNLMHYALSQLESVPDLTLYGPQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            10993..11409
                     /gene="sufE"
                     /locus_tag="JNC24_002691"
     CDS             10993..11409
                     /gene="sufE"
                     /locus_tag="JNC24_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HAY9159399.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLTELRDEDRSP
                     QNSIQGCQSQVWIVMRQNAHGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVSFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            11558..12562
                     /gene="ldtE"
                     /locus_tag="JNC24_002692"
     CDS             11558..12562
                     /gene="ldtE"
                     /locus_tag="JNC24_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HAY9159400.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIVPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(12625..12861)
                     /locus_tag="JNC24_002693"
     CDS             complement(12625..12861)
                     /locus_tag="JNC24_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006675846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major outer membrane lipoprotein"
                     /protein_id="HAY9159401.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
     gene            complement(13171..14583)
                     /locus_tag="JNC24_002694"
     CDS             complement(13171..14583)
                     /locus_tag="JNC24_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase I"
                     /protein_id="HAY9159402.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEQGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
     gene            15140..15349
                     /gene="fumD"
                     /locus_tag="JNC24_002695"
     CDS             15140..15349
                     /gene="fumD"
                     /locus_tag="JNC24_002695"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HAY9159403.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
BASE COUNT         3902 a         3737 c         4089 g         3717 t
ORIGIN      
        1 agctgaatgg cggtaagcag gaaaatatgg ggaagagcaa gcaaatatca attagcggtg
       61 attaacaatc aggcggcaga gccgcctgat gagaaattac ttattcggtt tctcgatttc
      121 gccaagttct tcgagaattt cttccggttg gtccgtcggc ggcgggactt cttccaccca
      181 cgcggcaaac agacgccagg aaacggctaa cagaaccgga ccaataaaca gaccgatcat
      241 cccgaaagca atcaaaccac caataacgcc agagaggatc aggatcagcg gtaaatcggc
      301 acccatgcga attaacattg ggcggatgac gttatccagc gtgccaacca caccgctcca
      361 cactaacaat accgttcccc aggtggtatc gccggtccag tagagccaga taatcgccgg
      421 aatcagtacc ggcaacgggc caagctggac aaggcaggag aggatcatta acaccgttag
      481 caacgttgca taaggtacgc cggatacggc gaggccgata ctgccaagca ctgcctgtac
      541 taacgccgtc accaccacac ccagcgccac cgcgcggata gcctgtgccg ccagcagcac
      601 ggcggcatct ccgcgaacgc ctgccagacg ggttgcaaaa tggcgaatgc cttgtgccac
      661 ctgttcaccg cgccagtaca gcagggcact gaagagaagc atcagcgcac aatgcaccat
      721 aaagcgcccg atatgcgccg cctgcccaac gaaccaggtg gtggtggtgc caatataagg
      781 gcggactttc gccatgatcg ccgtaccccc catatccagc aagttgtgcc agcctgcata
      841 cagcttcgcg ccaatcaccg gaatggtatt aagccacgcc agatcgggta acgtcatgtc
      901 gccgctggaa atggctttaa ttagcgggcc gctgccgtcg acgatactgt taaccagcaa
      961 agcaataggg atgataaaca ccatcactaa taacagcgtc atcaccagaa cggcgagaga
     1021 gcggcggcca aacatgatct tttgcaaacg taacaatacc ggccaggtgg cgataaccac
     1081 cgtaccggcc catgcaaagc cgagaataaa gggttgaaca atccacagac atgccacaat
     1141 catgatggct aaaaacagca ccgaaagcag aatttgtgcg acatccctgg gctgacgaac
     1201 atttaccata actacttttc accttttttg tgcgtcaaat cgtcgacgcg acgtgaacac
     1261 gcgaaactca cctgcataat gatgagcaat ttcagcggtt tttaacaggc cgattctgcc
     1321 cttaattctg atgggcgtgt tagaaaaaaa tgtggtacaa caactcaggc aacacgcaaa
     1381 cgatttactc gtcgtatttc aagttgcatg cgctgcggct acgttcgttc accccagtca
     1441 cttactattg taagctcctg gagttcactc gcttgccgcc ttcctgcaac tcgaaatcca
     1501 tagagtaaat aaacatcacc tttcagtgcg ctattgcagc cgcctgaaag aatagtgtat
     1561 cgacctacat cacagaccgc aggaaagggt caatataatg attccacaga tttcccaggc
     1621 acccggcgtc gttcaactgg tgcttaattt tttgcaagag ctggagcaac aaggttttac
     1681 cggcgatacg gcgacaagtt atgccgatcg tctgacaatg tcgaccgaca acagtattta
     1741 ccaacttctc cccgatgcgg tggtatttcc gcgttcaacc gcagatgtgg cgctgatcgc
     1801 ccgtcttgcc gcgcaggaac gctattcatc gctgatcttt accccccgcg gcggcggcac
     1861 cggcactaac ggtcaggcgc tcaaccaggg gattattgtt gatatgtccc gccatatgaa
     1921 ccgcatcatc gaaattaacc ctgaagaggg ctgggtgcgc gttgaggccg gggtgataaa
     1981 agatcaactc aatcagtacc tgaaaccgtt cggctacttt tttgcaccag aactttcgac
     2041 cagcaaccgg gcaacgctcg gcgggatgat caataccgat gcatccggtc agggatcgct
     2101 ggtctatggc aaaacgtcag atcacgtact tggcgtacgc gcggtgttgt tggggggcga
     2161 tattctcgat acgcaacctt tacccgtcga actggcggaa acgctgggta aaaccaatac
     2221 cacaatcggg cgaatttata acacggttta tcaacgttgc cgtctgcaac gccagttaat
     2281 catcgacaac ttccccaaac ttaaccgctt tcttaccggt tacgatctgc gtcatgtctt
     2341 taacgatgag atgaccgagt tcgacctgac gcgcattctg acgggttcag aagggacgct
     2401 ggcctttatt accgaagcgc ggctggatat tacgcccttg cctaaagtgc gccgtctggt
     2461 gaacgtcaaa tatgactctt ttgactccgc gctgcgtaac gcgccgttta tggttgaggc
     2521 gcgggcgctt tcggtagaga cggtggactc aaaagtgctg aatctggcgc gggaagatat
     2581 tgtctggcat tccgtcagcg agttgattac cgatgtgcct gacaaagaga tgctcgggct
     2641 gaacattgtg gaatttgctg gtgatgatga ggcgctgatt gacgagcgag ttaatgcact
     2701 ctgtgtgcgg cttgatgagc tgattgccag ccaacaggca ggtgtgattg gctggcaggt
     2761 gtgccgcgag ctggcggggg ttgaacgtat ctatgcaatg cgcaaaaaag ccgttggtct
     2821 gcttggcaat gccaaaggtg ccgctaagcc aattccgttt gctgaggata cctgcgtacc
     2881 gccggaacat ctggccgatt atattgctga atttcgcgcg ctgctcgaca gccacggctt
     2941 aagctacggt atgttcggtc acgtcgacgc aggtgttttg cacgtccgtc cggcactgga
     3001 tatgtgcgat ccgcaacaag agattttgat gaagcaaatc tctgatgacg tggtggcgct
     3061 gactgcgaaa tacggtggtt tgttgtgggg cgagcacggc aaaggttttc gcgctgaata
     3121 cagcccggcg tttttcggtg aggaactttt tgcagaactg cgcaaagtga aagcggcatt
     3181 tgacccgcat aaccgactca acccagggaa gatttgcccg ccagaaggtc tcgatgcgcc
     3241 gatgatgaaa gtggacgcgg tgaagcgcgg tacattcgat cggcagatcc ccattgcggt
     3301 acgccagcag tggcgcggtg cgatggagtg taacggcaac ggtttatgct tcaactttga
     3361 tgcccgtagt ccgatgtgtc cgtcgatgaa gatcacccag aaccggattc attcaccgaa
     3421 agggcgcgca acgctggtgc gtgaatggct gcgtttgttg gcggatcgcg gcgttgatcc
     3481 actcaaactg gaacaagaac tgcctgaatc cggcgtcagt ttacggacgt taattgcccg
     3541 cacgcgcaat agctggcatg cgaataaagg cgaatatgac ttctcacacg aagtcaaaga
     3601 ggcgatgtcg ggctgtctgg cctgtaaagc gtgttcgacc cagtgcccca tcaaaattga
     3661 tgtgccggag tttcgctctc gtttcctgca gctctatcac acccgttatt tacgcccgct
     3721 gcgcgaccac ctcgtcgcta cggtcgagag ctacgcaccg ctgatggcac gcgcgccgaa
     3781 aacctttaac ttcttcatta accagccgct ggtgcgcaaa ctctcggaaa aacatatcgg
     3841 catggttgat ttgccactgc tgtcggtccc ctcgctacaa caacaaatgg tggggcatcg
     3901 ctcggcaaac atgacgctgg aacagcttga agctctcaat gcagagcaga aagcgcgcac
     3961 ggtgttggtg gtgcaggacc cctttaccag ctattacgat gcgcaagtgg tggcggattt
     4021 tgtccgtctg gttgaaaaat taggtttcca gcctgtgtta ctgccatttt cgccaaatgg
     4081 caaagcccag catatcaaag gctttcttaa tcgttttgcg aagacggcga aaaagacggc
     4141 ggatttcctc aaccgtatgg ctaagcttgg gatgccaatg gtgggcgtcg atccggcgct
     4201 ggtactttgt tatcgcgatg aatataaact ggcgctgggc gaggaacgtg gcgagtttaa
     4261 cgtcttactt gcgaatgaat ggctggcaag cgcacttgag tcacatccag tggctacagt
     4321 cagcggtgaa tcatggtatt tctttggtca ctgtaccgag gttaccgctt tgccgggcgc
     4381 gccagcacaa tgggccgcga tatttgcccg ttttggcgcg aaactggaaa atgtcagcgt
     4441 gggttgctgc ggcatggcag ggacttacgg acatgaagcg aaaaaccatg aaaattcgct
     4501 cgggatctat gagttatcct ggcatcaggc tatgcagcga ctgccgcgta accgctgtct
     4561 ggcgaccgga tattcctgcc gtagccaggt aaaacgggtt gaaggcacgg gggtacgcca
     4621 tcctgtgcag gctttactgg agattattaa atgatatgga aacggaaaat caccctggaa
     4681 gcactgaatg ctatgggtga aggaaacatg gtggggttcc tggatattcg ctttgaacat
     4741 attggtgatg acacccttga agcgacaatg ccagtagact cgcggacaaa gcagcctttc
     4801 gggttgctgc atggaggagc atccgtggta ctggccgaaa gtatcggttc cgttgccggt
     4861 tatttatgta ccgaaggtga gcaaaaagtg gttggtctgg aaatcaatgc taatcacgtc
     4921 cgctcggcac gagaagggcg ggtgcgcggc atatgtaaac cgttgcatct cggttcgcgt
     4981 caccaggtct ggcagattga aatcttcgat gagaaagggc gtttgtgctg ttcgtcacga
     5041 ttgacgaccg ccattttgtg acgttgtcac cagaaaagtg tgacgcatat tgcttttggt
     5101 cattatctgc acagtaaacg gtggtatact gtgcacgtct tgtctaaaag cgaggatgct
     5161 atgtctactc aacttgatcc cacccagctg gccattgaat ttttacgccg tgaccagagc
     5221 aatctttcac ctgcccaata tctcaaacgt ctgaaacaac tagaacttga atttgctgac
     5281 ctgcttactc tctcttctac tgaattaaaa gaagaaatct actttgcctg gcgtctgggc
     5341 gtgcattgat cctgacttaa tgccgcaagt tcgccaaatc gacctggatt aacctccgtc
     5401 caggttcgat gaccttctta atactgctta tctaccgcct gatattgttc aaaaataata
     5461 ctaatcgcag tttgtgttaa aacggcgggt tagctttatg agattattct tatcgcccct
     5521 tcaagagcta agccactgag agtgccggag ataagcgccg gatggggtag aaacccttaa
     5581 gcctgtgtcg cacagactta agggttttct tatttctgaa cgctgcactt ttttacctat
     5641 ctcttaaata gtcattttca atacaacttt ttctttttca tcacagcctt aatgctcctt
     5701 agataataac cattatctaa caatgagata cctaattctt agcgtgcctg ttaacccaca
     5761 catcagggtc tatgcttatt aaaagcaggc aaaaagcatg ttttacggta aagcccctgc
     5821 gtttgctggg ttgaactgat aatcattatc actagcatgc tgttatacgc tgaaagcgat
     5881 gaagtgaggt aaatcgatgg acatgcattc aggaaccttt aacccacaag atttcgcctg
     5941 gcaaggctta acgctgacac ccgcagcggc gatacacatc cgtgagctgg tggcaaagca
     6001 gccgggtatg gtcggcgtgc gcttaggcgt gaagcaaacg ggctgcgcgg gctttggcta
     6061 tgtgctcgac agtgttagcg agccggacaa agacgatctg ctgtttgaac acgacggcgc
     6121 gaagctgttt gtcccgctgc aagcgatgcc gtttattgat ggcacggaag tcgatttcgt
     6181 tcgtgaagga cttaatcaga tattcaaatt tcacaaccct aaagcccaga atgaatgtgg
     6241 ctgtggcgaa agctttgggg tataggcggt actatgtctc gtaatactga agcaactgac
     6301 gatgtcaaaa cctggaccgg cggcccgctg aattataaag aaggattctt cacccagtta
     6361 gccaccgatg agctggcaaa ggggataaac gaagaggtgg tgcgcgcaat ttcggcgaag
     6421 cgtaatgagc cggagtggat gctggagttt cgtctaaacg cctatcgcgc atggctggag
     6481 atggaagaac cgcactggtt gaaagcgcac tacgacaagc tgaattatca ggattacagt
     6541 tactactcag caccatcgtg cggtaattgt gacgacactt gcgcgtctga acctggcgcg
     6601 gtgcagcaaa ctggcgcgaa cgccttttta agtaaagagg tggaggcggc gtttgagcag
     6661 ttgggcgttc ccgtgcggga aggcaaagag gtggcggtgg atgccatttt cgactctgtt
     6721 tcggttgcca ctacttatcg cgaaaaactg gcggagcagg gaattatttt ctgttccttt
     6781 ggtgaggcga tccacgatca tccggaactg gtgcgtaaat atctcggcac tgtggtgccg
     6841 ggaaatgaca acttctttgc cgcgcttaat gcggcggtag cctctgacgg tacgtttatt
     6901 tatgtgccta aaggcgtgcg ctgcccgatg gaactttcca cctattttcg cattaacgcg
     6961 gaaaaaaccg ggcagtttga gcgcaccatt ctggtggccg acgaagacag ctacgtcagc
     7021 tacattgaag gctgttccgc tccggtgcgt gacagctatc agttacacgc ggcggtggtg
     7081 gaagtcatca tccataaaaa cgccgaggtg aaatattcca cggtacaaaa ctggttccct
     7141 ggcgataaca acaccggcgg tattctcaat ttcgtcacca agcgtgcttt gtgcgaaggc
     7201 gaaaacagca aaatgtcatg gacgcaatca gaaaccgggt cagcgattac gtggaaatat
     7261 cccagctgca ttttgcgcgg cgataactcc attggtgagt tttactcagt ggcgctgacc
     7321 agcggtcatc agcaagcgga taccggcacc aagatgatcc acatcggtaa aaacaccaaa
     7381 tcgaccatta tctcgaaagg gatctctgcc ggacatagtc agaacagtta tcgcggctta
     7441 gtgaaaatca tgccgacggc aaccaatgcg cgcaatttca ctcagtgcga ctcaatgctg
     7501 attggcgcta attgtggggc gcataccttc ccgtatgtcg aatgtcgcaa taacagcgca
     7561 caactggagc acgaggcaac gacatcacgt attggtgaag atcaactgtt ttactgcctg
     7621 caacgcggga ttagcgaaga agacgccatc tcgatgattg ttaacggttt ctgcaaagac
     7681 gtgttctcag aactgccgct ggaatttgcc gttgaagcac aaaaactcct cgccatcagt
     7741 cttgaacaca gcgtcggata aggaataaac atgttaagta ttaaagattt acacgtcagc
     7801 gtggaagata aagctatcct gcgtggatta agcctcgacg ttcgtcccgg cgaagttcac
     7861 gccattatgg ggccaaacgg ttcgggcaaa agtaccttat cggcaacgct tgccgggcga
     7921 gaagattatg aagtgacggg cggcacggtt gagttcaaag gcaaagattt gcttgcgctg
     7981 tcgccggaag atcgtgcagg cgaaggcatc tttatggcct tccagtatcc ggtggagatt
     8041 cccggcgtca gtaaccagtt tttcctgcaa acggcactta atgcggtgcg cagctatcgc
     8101 ggccaggaaa cgctcgaccg ctttgatttt caggatttga tggaagagaa aatcgctctc
     8161 ctgaagatgc cggaagattt attaacccgt tcggtaaacg ttggtttttc cggcggcgag
     8221 aaaaagcgca acgatatttt gcaaatggcg gtgctggaac cggagttatg cattcttgat
     8281 gaatcggact ccgggctgga tattgacgca ttaaaagtgg ttgccgatgg cgtgaactcg
     8341 ctgcgtgacg gcaagcgctc attcatcatt gttacgcact accaacgcat tctcgactac
     8401 atcaagcctg attacgtcca tgtgctatat cagggacgaa ttgtgaaatc cggcgatttc
     8461 acgttggtca aacaactgga ggagcagggt tatggctggc ttaccgaaca gcagtaatgc
     8521 gctgcaacag tggcatcact tgtttgaagc tgaagggaca aaacgctcac cgcaagcaca
     8581 gcagcattta caacaattgc tgcgtaccgg actgccgaca cgtaaacatg aaaactggaa
     8641 atatacgccg ctggaagggc tgaccaatag tcagtttgtc agcattgcgg gagatatatc
     8701 cccacagcag cgtgatgcct tagcgttaac gttagacgcc gtgcggctgg tgtttgtcga
     8761 tggacgttac gtgcccgcgc tgagcgatgc aattgaaggc agcggatatg aagtgagcat
     8821 taacgacgac cgtcaggggt tacccgacgc tattcaggcg gaagtgtttc tgcatttgac
     8881 ggaaagcctg gcacaaagcg tgacgcatat cgccgtgaag cgcggtcaac ggccggcaaa
     8941 gccattgctg ttaatgcata tcacccaggg cgtggcaggt gaagaggtga acactgccca
     9001 ttaccggcat catctggatc tggcggaagg tgccgaagca acggtgatcg aacattttgt
     9061 cagccttaat gatgctcgtc attttaccgg ggcacggttc actatcaatg tcgcagcgaa
     9121 cgcccacttg cagcatatca agctggcgtt tgaaaacccg ctcagtcacc actttgctca
     9181 taacgatttg ttgctggctg aggatgccac cgcatttagc catagtttcc tgctgggtgg
     9241 cgcagtgtta cgacacaaca ccagtacgca actcaatggc gaaaacagca cgctgcggat
     9301 caatagcctg gcgatgccgg tgaaaaacga ggtgtgtgat acccgtacct ggctggaaca
     9361 caataaaggt ttttgtaaca gccgacagtt gcacaaaact atcgtcagcg acaaaggccg
     9421 cgcggtattt aacggtttga tcaacgtcgc gcagcacgcc atcaaaacgg atggtcagat
     9481 gaccaacaat aatctgctga tgggcaaaat ggcggaagtg gatacaaaac cgcagctgga
     9541 aatctatgca gatgatgtga aatgcagcca cggcgcgacg gtggggcgta ttgatgatga
     9601 acagatgttc tatctgcgct cgcgcgggat caatcagcag gatgcccagc agatgatcat
     9661 ttacgccttt gctgccgaac tgacggaagc actgcgtgat gaggggctta aacagcaggt
     9721 gctggcccga atcggtcaac ggctgccagg aggtgcaaga tgactttttc cgtcgacaaa
     9781 gtgcgggccg actttccggt gctttctcgt gaggtgaacg gtttgccgct ggcttatctc
     9841 gatagcgccg ccagtgcgca gaaaccaagc caggtgattg acgccgaggc tgagttttat
     9901 cgtcacggct acgcggcggt gcatcgcggt attcatacct taagcgccca ggcgaccgag
     9961 aaaatggaga acgtgcgcaa gcgggcatcg ctgtttatta atgcccgttc ggcggaagag
    10021 ctggtgttcg tccgcggcac gacggaaggg atcaatctgg tcgccaatag ttggggcaac
    10081 agcaacgtgc gggcgggcga taacatcatc atcagtcaga tggagcacca cgctaacatt
    10141 gttccctggc agatgctttg cgcacgcgtt ggcgcagagc tgcgtgtgat ccctctcaac
    10201 cccgacggta cgctgcaact ggagacgctg cctacgctgt ttgatgagaa aactcgcctg
    10261 ctggcaatta ctcatgtctc caacgtgctg ggaacagaaa atccgctggc ggaaataatc
    10321 acgcttgcgc accagcatgg cgcaaaagtg ctggtggatg gcgctcaggc ggtgatgcat
    10381 catccggtgg atgttcaggc gctggattgc gacttttacg tgttctccgg gcataaactg
    10441 tatggcccca ccggaattgg cattctttat gtgaaagaag ccttgttgca ggagatgccg
    10501 ccgtgggaag ggggcggttc tatgatcgcc accgtcagcc tgagtgaagg cactacctgg
    10561 accaaagcac catggcggtt tgaagccggt acacccaata ccgggggcat tattggtctt
    10621 ggcgcggcgc tggagtatgt ttcggcgctg gggcttaata acatagccga gtatgaacag
    10681 aatctgatgc attatgcgct atcacagctg gaatctgtac cggatctcac tctctatggc
    10741 ccacaaaaca ggcttggcgt tattgctttt aatctcggta aacaccacgc ctatgatgtt
    10801 ggcagttttc tcgataatta cggcattgct gtgcgtaccg gacatcactg cgctatgcca
    10861 ttaatggcct attacaacgt ccctgcgatg tgtcgggcgt cgctggccat gtataacacc
    10921 catgaagaag tggatcgtct ggtgaccggc ctgcaacgta ttcaccgttt gctgggataa
    10981 cagggaggca ctatggcttt attgccggat aaagaaaaat tgctgcgtaa ttttttacgc
    11041 tgcgccaact gggaagagaa atatctctac attattgagc tgggccagcg tctgactgaa
    11101 ttacgcgacg aagaccgaag cccacaaaat agcattcagg gctgccagag tcaggtgtgg
    11161 attgtcatgc gccagaatgc gcacggaatt attgaattgc agggcgacag cgatgcggcg
    11221 attgtgaaag ggcttattgc ggtagtcttt attctctacg atcagatgac gccgcaggat
    11281 attgtcagtt tcgatgtgcg tccgtggttt gaaaaaatgg cactcaccca acatctcacc
    11341 ccatctcgtt cacaaggtct ggaagcgatg attcgcgcaa ttcgcgccaa agccgctgca
    11401 cttagctaaa ctaaagggac agctttcatc cggttggctc acattgccgg atggtcaatg
    11461 tttacactga cctgcacagg agtgttttgg tttcaggtga acataagcgc catccgacgt
    11521 ttcagcgtga gtctccggca aatacaggag gtttacaatg aaacgcgcgt ctttgcttac
    11581 actcacgctt atcggcgctt ttagcgccat ccaggctgcc tgggcggttg attatccgct
    11641 accaccaacc ggaagccgac tggttgggca aaatcaaacg tatacggtgc aagaagggga
    11701 taaaaacctt caggctattg cccgacgttt tgatactgcg gcaatgttga tccttgaagc
    11761 caataacact atcgtcccgg tgccaaaacc tggtacgacg ataactattc cttcacaact
    11821 gttattacct gatgcaccgc gtcaggggat tatcgttaac cttgcagagc tgcgccttta
    11881 ttattatccg ccgggagaaa atattgtgca ggtttatcca ataggtattg gattgcaggg
    11941 gctggaaacg ccggtgatgg aaacgcgtgt tgggcagaaa atccctaacc caacctggac
    12001 gcctacggca ggcattcgtc agcgttcgct ggagcgtggc attaaattac cgccagtcgt
    12061 tcctgccgga ccaaataacc cgctaggacg ttacgcactg cgcctcgcgc atggtaatgg
    12121 cgaatacctc attcatggca ccagtgcgcc ggacagcgtt ggtttgcgcg tcagttcagg
    12181 gtgtattcgc atgaatgcac cggatattaa agccttgttc tccagcgtgc ggacgggaac
    12241 gccggtgaaa gtgatcaacg aaccggtgaa atattccgtg gaacctaacg ggatgcgtta
    12301 tgttgaagta catcgaccac tatcagcaga agaacagcaa aacgttcaga caatgccata
    12361 cacactgcca gcaggcttta cgcaatttaa agacaataag gctgtagatc agaagttagt
    12421 cgataaagcg ttgtatcgtc gggcagggta tccggtttcg gtgagcagtg gagcaactcc
    12481 cgcagccagc aatgcgcctt cagtagagtc agcgcagaat ggtgaaccag agcaagggaa
    12541 tatgttacgc gcgacgcagt agtggtaaat agcagacaaa aaaatggcgc acaatgtgcg
    12601 ccatttttca cttcacaggt actattactt gcggtattta gtagccatgt tgtccagacg
    12661 ctggttagca cgagctgcgt catctttagc agcctgaacg tcggaacgca ttgcgttcac
    12721 gtcgttgctc agctggtcaa ctttagcgtt cagagtctga acgtcagaag acagctgatc
    12781 gattttagcg ttgctggagc aacctgccag cagagtagaa cccaggatta ccgcgcccag
    12841 taccagttta gtagctttca ttattaatac cctctagatt gagttaatct ccatgtagcg
    12901 ttacaagtat tacacaaagt tttttatgtt gagaatattt ttttgatggg aaggcactta
    12961 tttttgatcg ttcgctcaaa gaagcatcga acgctgggtt ttagataaaa aaactgagag
    13021 aaaacaggat gcttccatcg gattcatctt agataaagtg agattatata gtgaaatccg
    13081 atttgatttt ttattgcttc tggttatcga ttaaataaaa aagcgcccat cagggcgctt
    13141 cgatatacaa attaattcac aaaagcaata ttacaggacg tgaacagatg cggtgttagt
    13201 agtgccgctc ggtaccagtg caccagaaac cataactacg acgtcacctt tgtgtgccag
    13261 accgctctgc agagccagtt ctttacccag acggtagaaa tcatcagtag aagtgatctc
    13321 tttaaccagc tgcggcacaa cgcctttgct cagtaccaac tgatgagccg ttttttcgtt
    13381 ggtggtcagt gccagtatgg tggcatccgg gaagtattta cgtactgcgc gagcagattt
    13441 accgccctgg gtagcaacca cgatcagcgg agcatccagt ttttcagcag tttcaacggc
    13501 accacggcat accgcttcgg taatgcgcag tttacggttg tcattgttga actcgagacg
    13561 gctgttcatc acgcggtcgg tacgttcgca gatggtcgcc atgatagaaa ccgcttccag
    13621 cgggtattta ccttttgcgg attcaccaga cagcatcact gcgtcagtac cgtcgaggat
    13681 ggcgtttgca acgtcaccgg cttctgcgcg agtcgggcgt gggtttttga tcatggaatc
    13741 cagcatctgg gtcgcagtga taacgacttt acgtgcacgg atacattttt cgatcatcat
    13801 cttctgggcg aagataactt cttctaccgg aatttctaca cccaggtcgc cacgcgcaac
    13861 catgatgccg tcagaggctt cgaggatttc gtcgaagttg ttgaggcctt cctggttttc
    13921 gattttggag atgatgtgga tgttttcgcc gccgtgcgct ttcaggtgct cacggatttc
    13981 gataacgtca gaacgcttac gaataaagga agcagcaaca aagtctacgc cttgttcgca
    14041 accaaagatc aggtcctgtt tgtctttttc agccagtgct ggcagggcaa tggaaacgcc
    14101 aggcaggttc acacctttgt tttcgcccag gtcgccgttg ttcagcactt tacagataac
    14161 tttgttacct tcaatggcag taacttccat accgatcaga ccatcgtcaa ccagtacggt
    14221 gttgccaaca gacaggtcag tagtgaaacc ttcatacgtt accgcaacca tttcgctgtt
    14281 gccgataaca gatttatcag tggtgaaagt aaaggtctga cccgctttca gagaaacgtc
    14341 gttaccgcct tccagtttca tggtgcggat ttccggacct ttggtatcaa gcaggatagc
    14401 ggcggtttta ccagttttgc tcatcacgtt gcgcagattc tgaatgcgct gaccgtgttc
    14461 tgcatagtca ccatgagaga agttcagacg cataacgttc atgccagcgt ccagcatttt
    14521 agctaacatc tcttcagatt cggttttcgg tccgatggtg caaacaattt tggtcttttt
    14581 catgacagtc ttagtcttta agttgagaag gatgggagaa acttgctttc tgggcgcact
    14641 gccggaaagc gaatccggta ttacgaaagt ggtggtgcct tgctctaagg ataggtgaca
    14701 tcgaaaaagc gtgcagagga atgtgtgctt gtgtctcagc ccaacgtgag tgcctgtttt
    14761 tgagtcgttg agttctacag tccaaagtgc tgaaaccatt caagagtcaa ttggcgcgca
    14821 ttatacgctg aaattaatcc aaaaggaaaa caaaaacagc gtttcaaaaa atatacaact
    14881 tgttagacaa taaattgtta tcaaagattt aacgttccag ggaaaaggtt acgcaaaaac
    14941 ttcgctgttt gcgcaacgtc tggtgccgaa gttgtcagtg tgaggataag tgaaagttca
    15001 tccggtgtat aaatatatca tcgctcaatt gctacagtta aaataattta tcgcgttatt
    15061 tgtctgattt tgtttgattt ttgtttaatt ggttagattg atatatgctg atagttggca
    15121 tgataaatag gtataaagaa tgggaaatcg gacaaaagag gatgaactgt accgagaaat
    15181 gtgcagagtt gtcggtaaag ttgtgctgga aatgcgcgat ctggggcagg aaccaaagca
    15241 tattgttatc gcaggcgtat tacgtactgc attagccaat aaacgcattc agcgcagtga
    15301 gctggaaaag caggcgatgg aaaccgtcat taacgcactg gtaaaatgac cattcaggat
    15361 gaggcggtaa gcttcatcct gatattcttg cttcagttgc ggtccagtta atcattctaa
    15421 ataacaactg ctgaaattat acgaa
//
LOCUS       DADMKF010000094        15407 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.096, whole genome shotgun sequence.
ACCESSION   DADMKF010000094 DADMKF010000000
VERSION     DADMKF010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15407
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.096"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            11..454
                     /locus_tag="JNC24_002696"
     CDS             11..454
                     /locus_tag="JNC24_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004997737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAY9159404.1"
                     /translation="MDLALVTGQREGDLAAMRWEDIRDGRLYVEQQKTGAKIRISLPT
                     TISRLNLTLADVLDNLKKINGKNEKLLGGKTARTIAAQFRIARDTSGLKWEGDPPPFH
                     EIRSLSGRLHSAEKGGDFTQALLGHRSSSMTDKYRDGRGREWKDI"
     gene            631..759
                     /locus_tag="JNC24_002697"
     CDS             631..759
                     /locus_tag="JNC24_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005141289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAY9159405.1"
                     /translation="MVSVYHSRRRKFLNDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            complement(760..1818)
                     /gene="modC"
                     /locus_tag="JNC24_002698"
     CDS             complement(760..1818)
                     /gene="modC"
                     /locus_tag="JNC24_002698"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HAY9159406.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMS
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLDEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(1821..2510)
                     /gene="modB"
                     /locus_tag="JNC24_002699"
     CDS             complement(1821..2510)
                     /gene="modB"
                     /locus_tag="JNC24_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HAY9159407.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPGETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(2510..3283)
                     /gene="modA"
                     /locus_tag="JNC24_002700"
     CDS             complement(2510..3283)
                     /gene="modA"
                     /locus_tag="JNC24_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY9159408.1"
                     /translation="MARKWLNLFAGAALSFAVAGNTLADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTASRQ
                     TLLGNSLVVVAPKASEQKDFIIDSTTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVVSKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(3449..3598)
                     /locus_tag="JNC24_002701"
     CDS             complement(3449..3598)
                     /locus_tag="JNC24_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump-associated protein, AcrZ
                     family"
                     /protein_id="HAY9159409.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            3727..4515
                     /gene="modE"
                     /locus_tag="JNC24_002702"
     CDS             3727..4515
                     /gene="modE"
                     /locus_tag="JNC24_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HAY9159410.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDIQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            4583..6055
                     /gene="modF"
                     /locus_tag="JNC24_002703"
     CDS             4583..6055
                     /gene="modF"
                     /locus_tag="JNC24_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HAY9159411.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPGEDDTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     ESFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDEIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANEPRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLVTGDHPQGYSNDLTLFGRRRGSGETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     RTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            6316..7332
                     /gene="galE"
                     /locus_tag="JNC24_002704"
     CDS             6316..7332
                     /gene="galE"
                     /locus_tag="JNC24_002704"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308814.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HAY9159412.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVGESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPKIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            7342..8388
                     /gene="galT"
                     /locus_tag="JNC24_002705"
     CDS             7342..8388
                     /gene="galT"
                     /locus_tag="JNC24_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HAY9159413.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            8392..9540
                     /gene="galK"
                     /locus_tag="JNC24_002706"
     CDS             8392..9540
                     /gene="galK"
                     /locus_tag="JNC24_002706"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HAY9159414.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLNAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            9534..10574
                     /gene="galM"
                     /locus_tag="JNC24_002707"
     CDS             9534..10574
                     /gene="galM"
                     /locus_tag="JNC24_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HAY9159415.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKSCPVNMTNHVYFNLDGEQSDVRNHKLQILAGEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            10777..11529
                     /gene="gpmA"
                     /locus_tag="JNC24_002708"
     CDS             10777..11529
                     /gene="gpmA"
                     /locus_tag="JNC24_002708"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAY9159416.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(11688..12740)
                     /gene="aroG"
                     /locus_tag="JNC24_002709"
     CDS             complement(11688..12740)
                     /gene="aroG"
                     /locus_tag="JNC24_002709"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HAY9159417.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPVTENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLDSGEPLAYGKSITDACIGWEDT
                     DALLRQLANAVKARRG"
     gene            13056..13436
                     /locus_tag="JNC24_002710"
     CDS             13056..13436
                     /locus_tag="JNC24_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9159418.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEKHKNTMCKDGRCPD
                     INKKVQIGDGINNDVDTKTDGTTQ"
     gene            13550..14491
                     /gene="zitB"
                     /locus_tag="JNC24_002711"
     CDS             13550..14491
                     /gene="zitB"
                     /locus_tag="JNC24_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HAY9159419.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSIVVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVITLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHATIQMEYQPCHGPDCHLNEGVSGHSHHHH"
     gene            complement(14488..15207)
                     /locus_tag="JNC24_002712"
     CDS             complement(14488..15207)
                     /locus_tag="JNC24_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HAY9159420.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(15245..>15407)
                     /locus_tag="JNC24_002713"
     CDS             complement(15245..>15407)
                     /locus_tag="JNC24_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HAY9159421.1"
                     /translation="CAHCPWMAMNGLQSIAEALEQEGSNHEVLDERLRERALVPLNRM
                     LDFAATLRG"
BASE COUNT         3917 a         4044 c         3850 g         3596 t
ORIGIN      
        1 cggcctttgc atggatcttg cactggtaac aggacagcgc gaaggagatc tggcggctat
       61 gagatgggag gatataagag atggcaggct atacgtggaa caacaaaaaa caggggcaaa
      121 aattcgcatt tctctcccca ccacgatttc cagattgaat ttaacacttg ccgatgtact
      181 ggataacttg aaaaaaataa acggtaaaaa cgaaaagtta ctcggaggga agacagccag
      241 gacaatcgca gcacagttca ggattgcccg agacacatcc ggattgaaat gggaaggtga
      301 cccaccgcca tttcatgaaa tccgaagttt atccggtcgc ctgcactcag cagaaaaagg
      361 cggtgatttc actcaggcgc tccttggaca tcgttcatcc agcatgacgg ataaataccg
      421 tgacggacga gggagggaat ggaaagacat ttaaaattaa tttcgaatga tttcgaaaca
      481 ccacttgtaa ttaattgatt ataaatgatt aattcctaat gggcatttgc ttcatgaatg
      541 ctcctttgca tttagcaact gactgtaaca gctaaaatta gtcgcttttg gcggtgaatg
      601 ctgaattaaa acgcagaatt ttgtccgata gtggtatcgg tgtaccattc gcgcagacga
      661 aaattcttaa atgacgcggt aaaagcgcgc gccaacattg tgattggtga taacaccacc
      721 gacagcattg cccagttcat ttatagccac ctgatttaat caggcggtta tcgacacact
      781 tttaatttgc gcgtacagcc acaggccagg tttgatcgcc agttcatccc tggcccacgg
      841 gctgatacgc gcccacagcg ttttaccgcc gacttccagc tccacttcca cctggccgtt
      901 gtcgtcataa ctattaacaa cttttgcccg caatacgtta cgaatgctgg tttgctgcgg
      961 cggttgtaaa accagagaaa catcggaagc ctgaatgcgg atacgtagcg cagcttgcag
     1021 cggttcgtcc agcttattga cccacaaatg ctgatcgccc agcgccagcg cggtcatcgc
     1081 gtaatgcgga tgatgctcaa gtaccgtcac tttcagaatg ctactttgtt gctctttcgg
     1141 cagccacgga ttcatcacgc tactgcccca cacttcctcc agcgcgccaa aggctttcac
     1201 ctgaccgttt tccagtacca tcactctgtc tgccagatgg aggatctcat ccagcgaatg
     1261 gctgacatac aacatcggaa tgttgatttc ccgtgtcagc cgttgcagat aaggcaacag
     1321 ttcgcgttta cgcggaatat ccagtgacgc cagcggttca tccagcaaca gcaattccgg
     1381 tgctgtcagc aaagcccgac caatcgccac gcgctgtttt tcgcctccgg acaggctgcc
     1441 tggtaaacgg tcaagcaacg gttcaatgcc taaaagcgcc accagcttat cgaactgatc
     1501 gaccatactt ttcgacatgc cgtagcgcag attgccacgc actttgtaat gcgggaacag
     1561 ccgcgcatcc tgaaaaacat agccaacgcg acgcttttcc ggcgtcaggc agataccttt
     1621 ttcggcatca tttagtaccc gcccattgag gacaatccgc cctttttgcg ggcgcgtcag
     1681 tccactgatg gcgttaatca gcgaagtttt tccggcaccg gagacgccaa agatagcagt
     1741 gatgccattg gcgggcagtg tttcattaat ggtcaggcaa tggttgccca acgtctggga
     1801 aaaattcagc tccagcatga ttagcgcccc gcccgttcac ggctgattct ggccagccat
     1861 tctgaaatca acagggagat catcgccagc gcaatagaaa taatgcacag tctcgccgcg
     1921 ccactttccc cgccgggggt ctggatcagg gtatacatgg cagagggaat ggttcgcgtt
     1981 tcgccaggaa tgttcgacac aaaggtgatg gttgcaccaa actcaccgag agaacgagca
     2041 aaagccagta ccgtaccaac aataattccc ggtaaggtca gcggtaacgt gatagtaaag
     2101 aaaacgcgcc agcgcccggc acctagtgtt cttgcggcct gttcgagttt gacatcaacc
     2161 ccttccagcg ccagacgaat tgcccgcacc atcagcggaa acgacatgac ggcggcagcg
     2221 agaaccgcgc cgcgccagct aaaggcgaag gtaataccaa accagtcata cagacgttca
     2281 ccgataaatc cgcgccgtcc catcgaaact aataataagt aaccgacgac cacgggcggt
     2341 aacaccagcg gtagatgcag tacgctgtcg agcagagctt tgcccggaaa cgtgcaacgc
     2401 accagtaacc aggcaaaaaa gatcccaaac ggcaggctaa acagcacagc cagggaagaa
     2461 actttcaggc ttaataaaac tgcctgccat tctggatcgg tcagtatcat tacttggttg
     2521 taaatccgta acgtttaaag atttccgctg cctgcggtcc cttcagataa tcataaaatg
     2581 ctttcactgt cgcattgtta tgcccttcca caacagcaac cggatattcc acttttttat
     2641 gtgaatcttc cgggaaggtg gcaaccactt ttaccccttt gctgacaact gcgtcagaac
     2701 cgtaaacaat gcccaacggc gcttcattgc gttcgaccaa cgccagcgcg ccacgaacat
     2761 cttccgccgg agccagtttc ggggagagcg tatcccatgc gcccagtttt tgcagtgctt
     2821 cttttgcata aatgccagca gggacatgtt ccggatcgcc aaccgcgagg cgaccgccat
     2881 tcagcagcga agtccagtta gttgtgctgt cgatgatgaa atctttctgc tcgctggctt
     2941 tcggtgctac aacgaccagg ctattgccga gcagtgtctg gcgcgaagcc gtatcgatcg
     3001 cttttttatc aaccgcataa tccatccatt tctgatcggc agagataaac agatccgcag
     3061 gcgctcccgc ttcaatctga cgggccagag tagatgacga agcgaaagaa gaaaccacat
     3121 ccacgccttt ttctttttta tactgcgtag caatgtcctg cattgcgtta gtcagtgatg
     3181 cggcggcgaa caccgtgatt ttcccttcat ctgccagtgt attgccagca acagcgaaag
     3241 agagtgccgc cccggcaaac aagttcaacc atttacgagc cattggtaac cccttaatgt
     3301 aacgttatat aggtgacaat gtaacggcta gctcagaatt ttcccagcag ttattgctaa
     3361 cctttgtgag gtagataaga aaaaatatcg gctggaaaag caggaagttg agaaaaagaa
     3421 atgcccgact aagcgggcat tcagggaatc aatgattttg tccgggctgg tcttttttac
     3481 caacaccaga aaagatgttg aatacttcac caagaccgta aatcagaccc aggatgatgg
     3541 ccatcacgac aggtaccatg attacggcga ataccagact ttttaataac tctaacatgg
     3601 tcaactccag atatagtcat gagactattc taaccgctaa gcacagaaaa gcactcccct
     3661 tttgtgcggt cagctttgcg tggcgttcgt tttccgtcac aataagactt ttgccaggac
     3721 attgttatgc aggccgaaat ccttctcacc cttaagctcc aacaaaaatt attcgccgac
     3781 ccgcgccgga tttcgctact aaaacacatt gcgctttccg gttccattag ccagggagcg
     3841 aaagatgccg gtatcagcta taaaagcgcc tgggatgcca ttaacgagat gaatcagtta
     3901 agcgagcata ttctggttga gcgcgcaaca ggcggtaaag gtggcggcgg cgcagtactg
     3961 acccgctatg gtcagcgact gattcagctc tatgacttac tggcgcaaat ccagcaaaaa
     4021 gcctttgatg tgttaagtga cgatgacgcc ctgccgctga acagcctgct ggccgcgatc
     4081 tcacgttttt cactgcaaac cagcgcccgt aaccagtggt tcggtaccat caccgcccgc
     4141 gatcatgatg acattcaaca gcatgttgat gtcttactgg ctgacggaaa aacacgcctg
     4201 aaagtcgcaa ttaccgcaca aagcggcgca cgtctggggc tggatgaagg caaagaagtg
     4261 ttgatattgc taaaagcgcc gtgggtaggc attactcagg acgaagcggt cgcacaaaac
     4321 gccgacaacc aattaccggg tattattagt catattgagc gcggcgcaga gcagtgcgaa
     4381 gtattaatgg cgctacccga cgggcaaaca ctgtgcgcca cagtgccggt aaatgaagcg
     4441 acttctcttc agcaagggca gaatgtcacg gcctacttta atgccgacag cgtgattatc
     4501 gccacgctgt gctaagcgtg ttgacaattt gttatgaaac acgtatccct gtcagtaatc
     4561 gctgcacaaa atgggatata aaatgtcatc gttgcaaatt ttgcaaggca cgtttcgtct
     4621 tagcgacaca aaaacgctgc aattgcctca gctaacgtta aacgcgggtg atagttgggc
     4681 gtttgtcggt tcgaatggaa gcgggaaatc ggccctggcc cgcgcgctgg cgggggaact
     4741 tccgcttttg aaaggtgaac ggcaaagcca gttttcccac atcactcgtc tctccttcga
     4801 gcaattgcaa aaactcgtca gcgacgaatg gcagcggaat aacaccgata tgctcggccc
     4861 tggcgaagat gacaccggac gcactacggc tgaaatcatt caggatgaag taaaggatgc
     4921 accgcgttgc atgcaactgg cgcagcagtt cggtattacc gccctcctcg accgacgctt
     4981 taaatacctt tccactggcg agacgcgaaa aaccctgctg tgtcaggcgc tgatgtcgga
     5041 gcctgacttg ttgattcttg atgagtcgtt cgatggcctg gatgttgcct cacgtcagca
     5101 gctggctgag cgactcgcct cgttacatca gtccggtatt actctggtac tggtgctcaa
     5161 tcgcttcgat gagatcccgg agtttgtcca gtttgctggc gtgctggcgg attgcacgtt
     5221 agcggaaact ggcgctaaag aggaactgct ccaacaagca ctcgtcgcgc aactggcgca
     5281 tagtgaacag cttgaaggtg tgcaactgcc ggagccggat gaaccttcag cacgtcacgc
     5341 cttacccgcc aacgaaccgc gcattgtgct gaacaatggc gtggtttctt ataacgatcg
     5401 ccccattctt aataacctta gctggcaggt gaatccaggt gaacactggc aaattgtcgg
     5461 gccaaatggt gcaggaaaat cgacgttatt aagcctggtt actggcgatc atccgcaagg
     5521 ttacagcaac gatttgacgc ttttcggacg acgtcgcggc agcggcgaaa ccatctggga
     5581 tatcaaaaag catatcggtt acgtcagcag tagtttgcat ctggattacc gggtcagcac
     5641 taccgtgcgt aatgtgattc tttctggcta ttttgattcg attggcattt atcaggccgt
     5701 ttcggatcgc cagcaaaaac tggtgcagca gtggctggat attctcggca ttgataaacg
     5761 tacggctgac gctccgttcc atagtctttc ctggggacag cagcgtctgg cgctgatcgt
     5821 ccgcgcactg gtgaaacatc cgacgttgct tattctcgat gaaccactac aggggcttga
     5881 tccgctcaat cgccagctta tccgccgttt tgttgatgtg ctgattagcg aaggtgaaac
     5941 gcaattgtta tttgtttcgc accacgctga agatgcgcct gcctgtatta cccatcgcct
     6001 tgagttcgtg ccggacggtg gactctatcg ctatgtgctg acaaaaatat attgagtcgg
     6061 tagtgctgac cttgccggag gcggcctaag cgccctctcc ggccaacggt tcgacgcatg
     6121 caggcatgaa accgcttctt ttttcagata aaaagcgcaa tcattcataa accctctgtt
     6181 ttataatcac ttaatcgcgc ataaaaaacg gctaaattct tgtgtaaacg attccactaa
     6241 tttattccat gtcacacttt tcgcatcttt gttatgctat ggttatttca taccataagc
     6301 ctaatggagc gaattatgag agttctggtt accggtggta gcggttacat tggaagtcat
     6361 acctgcgtgc aattactgca aaacggtcat gatgtcatca ttcttgataa cctctgtaac
     6421 agtaagcgca gcgtactgcc tgttatcgag cgcttaggcg gcaaacatcc aacgtttgtt
     6481 gaaggcgata ttcgtaacga agcgttgatg accgagatac tgcacgatca cgctatcgac
     6541 accgtgatcc acttcgccgg gctgaaagcc gtcggcgaat cggtacaaaa accgctggaa
     6601 tattacgaca acaatgtcaa cggcactctg cgcctgatta gcgccatgcg cgccgctaac
     6661 gtcaaaaact ttatttttag ctcctccgcc accgtttatg gcgatcagcc caaaattcca
     6721 tacgttgaaa gcttcccgac cggcacaccg caaagccctt atggcaaaag caaattgatg
     6781 gtggaacaga tcctcaccga cctgcaaaaa gcccagccgg actggagcat tgccctgctg
     6841 cgctacttca acccggttgg cgcgcatccg tcgggcgata tgggcgaaga tccgcaaggc
     6901 attccgaata acctgatgcc atacatcgcc caggttgctg taggccgtcg cgactcgctg
     6961 gcgatttttg gtaacgatta tccgaccgaa gatggtactg gcgtacgcga ttacatccac
     7021 gtaatggatc tggcggacgg tcacgtcgtg gcgatggaaa aactggcgaa caagccaggc
     7081 gtacacatct acaacctcgg tgctggcgta ggcagcagcg tgctggacgt ggttaatgcc
     7141 ttcagcaaag cctgcggcaa accggttaac tatcattttg caccgcgtcg cgaaggcgac
     7201 cttccggcct actgggcgga cgccagcaaa gccgaccgtg aactgaactg gcgcgtaacg
     7261 cgcacactcg atgaaatggc gcaggacacc tggcactggc agtcacgcca tccacaggga
     7321 tatcccgatt aaggaacgac catgacgcaa tttaatcccg ttgatcatcc acatcgccgc
     7381 tacaacccgc tcaccgggca atggattctg gtttcaccgc accgcgctaa gcgcccctgg
     7441 cagggggcgc aggaaacgcc agccaaacag gtgttacctg cgcacgatcc agattgcttc
     7501 ctctgcgcag gtaatgtgcg ggtgacaggc gataaaaacc ccgattacac cgggacttac
     7561 gttttcacta atgactttgc ggccttgatg tctgacacgc cagatgcgcc agaaagcaac
     7621 gatcctttga tgcgttgcca gagcgcgcgc ggtaccagcc gggtgatctg cttttcaccg
     7681 gatcacagta aaacgttgcc agaactgagc gttgcggcat tgacggaaat cgtcaaaacc
     7741 tggcaggagc aaaccgcaga gctgggaaaa acgtacccgt gggtgcaggt ctttgaaaac
     7801 aaaggtgcgg cgatgggctg ctctaacccg catccgcacg gacagatttg ggcaaatagc
     7861 ttcctgccta acgaagctga gcgcgaagac cgcctgcaaa aagaatattt tgccgaacag
     7921 aaatcaccaa tgctggtgga ttatgttcag cgcgagctgg cagacggtag ccgtaccgtt
     7981 gtcgaaaccg aacactggtt agccgttgtg ccttactggg ctgcctggcc gttcgaaacg
     8041 ctactgctgc ccaaagccca cgttttgcgg atcaccgatt tgaccgacgc ccagcgcagc
     8101 gatttggcgc tggcgttgaa aaagctgacc agtcgttatg acaacctctt ccagtgctcc
     8161 ttcccctact ctatgggctg gcacggcgca ccatttaatg gcgaagagaa tcaacactgg
     8221 cagctgcacg cgcactttta tccgcctctg ctgcgctccg ccaccgtacg taaatttatg
     8281 gttggttatg aaatgctggc agagacccag cgagacctga ccgcagaaca ggcagcagag
     8341 cgtttgcgcg cagtcagcga tatccatttt cgcgaatccg gagtgtaaga aatgagtctg
     8401 aaagaaaaaa cacaatctct gtttgccaac gcatttggct accctgccac tcacaccatt
     8461 caggcgcctg gccgcgtgaa tttgattggt gaacacaccg actacaacga cggtttcgtt
     8521 ctgccctgcg cgattgatta tcaaaccgtg atcagctgtg caccacgcga tgaccgtaaa
     8581 gttcgcgtga tggcagccga ttatgaaaat cagctcgacg agttttccct caatgcgccc
     8641 attgtcgcgc atgaaaacta tcaatgggcg aactacgttc gtggcgtggt gaaacatctg
     8701 caactgcgta acaacagctt cggcggtgtg gacatggtga tcagcggcaa tgtgccgcag
     8761 ggtgccgggt taagttcttc cgcttcactg gaagtcgcgg tcggaaccgt attgcagcag
     8821 ctttatcatc tgccgctgga cggcgcacaa atcgcgctta acggtcagga agcagaaaac
     8881 cagtttgttg gctgtaactg cgggatcatg gatcagctaa tttccgcact cggcaagaaa
     8941 gatcatgcct tgctgattga ctgccgctca ctggggacta aagcagtttc catgccgaaa
     9001 ggtgtggctg tcgtcatcat caacagtaac ttcaaacgta ccctggttgg cagcgaatac
     9061 aacacccgtc gtgaacagtg cgaaaccggt gcgcgtttct tccagcagcc agctctgcgc
     9121 gatgtcacca ttgaagagtt caacgctgtt gcacatgagc tggacccaat cgtggcgaaa
     9181 cgcgtgcgtc atatcctgac tgaaaacgcc cgcaccgttg aagctgccag cgcgctggag
     9241 caaggcgatc tgaaacgtat gggcgagttg atggcggagt ctcatgcctc tatgcgcgat
     9301 gatttcgaaa tcaccgtgcc gcaaattgac actctggtag aaatcgtcaa agcagtgatt
     9361 ggcgacaaag gtggcgtacg catgaccggc ggcggatttg gcggctgtat cgtcgcgctg
     9421 atcccggaag agctggtgcc tgccgtacag caagctgtcg ctgaacaata tgaagcaaaa
     9481 acaggtatta aagagacttt ttacgtttgt aaaccatcac aaggagcagg acagtgctga
     9541 acgaaactcc cgcactggca cccgatggtc agccgtaccg actgttaact ttgcgtaaca
     9601 acgcagggat ggtagtcacg ctgatggact ggggtgcgac tttactttca gctcgtattc
     9661 cgctttccga tggcagcgtc cgcgaggcgc tgctcggctg tgccagcccg gaatgctatc
     9721 aggatcaggc cgcgtttctg ggggcctcta ttggtcgtta tgccaaccgc atcgccaata
     9781 gccgttatac ctttgacggt gaaacggtga cgctttcgcc aagtcagggc gttaaccagc
     9841 tgcacggcgg cccggaaggg ttcgacaaac gtcgctggca gattgtgaac cagaacgatc
     9901 gtcaggtgct gtttgccctg agttcagatg atggcgatca gggcttcccg ggtaatctcg
     9961 gcgcgacggt gcaatatcgt ctgaccgacg ataaccgtat ctccattact tatcgcgcca
    10021 cagttgataa atcttgcccg gtgaatatga ctaatcacgt ctatttcaat cttgacggcg
    10081 agcagtctga cgtgcgcaat cacaagttgc agattctggc gggcgaatat ctgccggttg
    10141 atgaaggcgg cattccgcac gacggcctga aatctgtcgc cggaacctct tttgatttcc
    10201 gcagcgccaa aatcatcgcc agtgagtttc ttgccgacga cgatcagcgc aaagtgaaag
    10261 gttacgatca cgcattcttg ttacaggcca aaggcgatgg caagaaagtg gcggcgcatg
    10321 tctggtcagc agatgaaaaa ttgcagctga aggtctacac caccgctccc gctcttcaat
    10381 tctactccgg caacttcctc ggcggcacac cgtcgcgggg aaccgaacct tacgccgact
    10441 ggcaaggact ggcgttggaa agcgagtttc tgccggacag cccgaaccac cctgaatggc
    10501 cgcaaccgga ctgcttcctg cgtcctggcg aagagtattc cagcctgacg gaatatcagt
    10561 ttattgctga gtaatgttaa gccctcgtca tgagggcttt atcttcatat tgttcaaatc
    10621 accagcaaac accgacatat ttgcaactca atattcacaa caaccttaca ctgcgccact
    10681 attttcgcta tggttatgcg taagcattgc tgttgcttcg tcacggcaat ataatgagaa
    10741 ttattatcat aaaaagataa tttgaggagt aagtatatgg ctgtaactaa gctggttctg
    10801 gttcgtcatg gcgaaagtca gtggaacaaa gaaaaccgtt tcaccggttg gtacgacgtg
    10861 gatctgtctg agaaaggcgt aagcgaagca aaagcagcag gtaagctgct gaaagaggaa
    10921 ggttacagct ttgactttgc ttacacttct gtgctgaaac gcgctatcca taccctgtgg
    10981 aatgtgctgg acgaactgga tcaggcatgg ctgcccgttg agaaatcctg gaaactgaac
    11041 gaacgtcact acggtgcgtt gcagggtctg aacaaagcgg aaaccgccga aaagtatggc
    11101 gacgagcagg tgaaacaatg gcgtcgtggt tttgccgtga ctccgccgga actgactaaa
    11161 gatgatgagc gttatccggg tcacgatccg cgttacgcga aactgagcga gaaagagctg
    11221 ccgctgactg aaagcctggc gctgaccatt gaccgcgtga tcccttactg gaatgaaact
    11281 attctaccgc gtatgaagag cggtgagcgc gtgatcatcg ctgcacacgg taactcttta
    11341 cgtgcgctgg tgaaatatct tgataacatg agtgaagaag agattcttga gctgaatatc
    11401 ccgaccggcg tgccgctggt gtatgagttc gacgagaatt tcaaaccgct gaaacgctat
    11461 tatctgggta atgctgacga gatcgcagcg aaagcagcgg cggttgctaa ccagggtaaa
    11521 gcgaagtaaa cgtcattcgt ttaaaaatga gaaagccgac tgcaagtgag tcggcttttt
    11581 tgttgctaac aatggagcac attgcctgat gcgacgctgc gcgtcttatc aggcctgtgg
    11641 tgattcatcg gatacgccac tctgacggcg catccgacaa ttaaacctta cccgcgacgc
    11701 gcttttactg cattcgccag ttgacgtaac agagcatcgg tatcttccca gccaatgcag
    11761 gcatcggtga tgctcttacc gtaggccagc ggctccccgc tatcgaggct ctgattgcct
    11821 tccaccagat ggctttccac catcacgcca ataatggcct tttcgccacc ggcaatctgc
    11881 tggcaaacgt cagcacaaac atccatctgc tttttgaatt gtttggacga gttagcatgg
    11941 ctgaaatcga tcatcacctg tgctggcagg cctgctttgt tcagcccttc tttcacttca
    12001 gcaacgtgct tcgcgctgta gttaggctct ttaccgccgc gcagaatgat atggcaatca
    12061 ccgttaccgc tggtattcac aatcgccgaa tgcccccatt tagttacgga caggaagcag
    12121 tgcggcgcac cggcggcatt aatggcatcg atagccactt taatcgtacc gtctgtgcca
    12181 tttttaaaac ctaccggaca agaaagacca gacgccagtt cgcggtgcac ctgcgattcc
    12241 gtggtacgtg caccaattgc gccccagctc atcaggtcag cgagatactg aggagtgatc
    12301 atatcgagaa actcacccgc cgctggcaga ccgctgtcgt taatatcaag cagcaattta
    12361 cgggctatac gcagaccgtc gttgatctgg aagctgttat ccatatgcgg atcgttaatc
    12421 aaccctttcc agcccaccgt ggtacgcggc ttttcaaaat agacgcgcat tacgatttcc
    12481 agctcatctt tcagctcttc acgcagcgcc agcaagcgag tggcatactc tttagccgcg
    12541 acaggatcat gaattgagca cgggccaatc accaccaaca ggcgatcatc attacctttc
    12601 aggatcttat ggatcgcttt tcgggcatgg gcaaccgtat tcgcggcatt ttcagttacg
    12661 gggaattttt ccagcaatgc gacaggagga agtaactctt tgatttcttt gatgcgtaaa
    12721 tcgtcgttct gataattcat gtctgttcca gtgttgccat acttatctta gtgaatgcaa
    12781 tacttccaat ctatatcttc cgtcagaatg tgtaaacggg gttttacact atgaacggat
    12841 taatcctgga attagcaaga aaaacgccag attgtcgcga aaaacgagat ctctcctaca
    12901 atttctaact gtaactcctt tgtttgttaa ttatttcaag attctctgct gcgtttcata
    12961 aactggctga aatcttaaac caatgcctta tattcacctg caaatgcact gttggaagag
    13021 gttatccgac ataacgacca taacaggagc atcctatgaa aatgacaaaa ctggccacat
    13081 tatttctgac tgccactcta agccttgcca gcggtgccgc actggctgcc gatagcggag
    13141 cgcaaactaa taacggccag gcaaacgccg cagctgatgc gggccaggta gcccctgacg
    13201 cccgtgaaaa tgtcgcgcca aataacgtcg acaataacgg ggtaaatacc ggttctggcg
    13261 gcacaatgct gcattcggat ggttcttcaa tgaacaatga cggaatgacc aaagatgaaa
    13321 agcacaagaa caccatgtgc aaagatggtc gatgtccgga tatcaataaa aaggtacaaa
    13381 tcggtgatgg catcaataat gatgtcgata ccaaaaccga cggtaccacg cagtaaccct
    13441 gcaaaatagc caataatggg agagcggagt ctctcccttt taatttttca cttctaaaag
    13501 cagtatgatg tcataaagtt aagtgagaat aattaaggga tgactcgtca tggcgcactc
    13561 acactcacac acgtcctcac acctgccaga agataataat gctcgtcgct tgttgtatgc
    13621 tttcggcgtg actgccgggt ttatgctggt agaagtcgtt ggtggttttc tttctggttc
    13681 tctggcattg ctggccgatg cgggtcatat gttgaccgat actgccgccc tgcttttcgc
    13741 cctactcgcc gtgcaatttt cacgtcgtcc tcccaccatt cgccacactt tcggttggct
    13801 aagactgacc acgctcgcgg cgtttgtgaa tgccatcgcc ctcgtggtaa ttaccatttt
    13861 gattgtctgg gaggcgatag aacgcttccg cacgccgcgt ccggtcgagg gcggcatgat
    13921 gatggcaatt gccgtggctg ggttgctggc aaatatactt tctttctggt tacttcatca
    13981 cggcagcgaa gagaaaaacc tcaacgtacg agcagcggca ttgcatgtgc tcggggacct
    14041 gttgggttcg gttggggcta ttattgccgc gttgattatt atctggaccg gctggacacc
    14101 tgctgacccc attctctcga tagtggtgtc gcttctggtt ctgcgtagcg cgtggcgact
    14161 gttgaaagat agcgtgaatg aattacttga aggtgcaccg gtatcgctgg atatcgctga
    14221 actgaagcgt cgtatgtgcc gggaaatccc ggaagtccgc aatgtgcacc atgtacatgt
    14281 atggatggta ggcgagaagc cggtgataac gctgcatgtg caggtgatcc cgccacacga
    14341 tcacgatgcc ttgttggatc agatccaaca ctacctgatg gatcactatc agattgagca
    14401 cgccaccatt cagatggaat atcaaccttg tcatgggcca gactgccacc tgaatgaagg
    14461 ggtgtctggc cattcacatc atcaccatta atgggacagc gcgcgtgagc ctctttcacg
    14521 tgcgctgttg atccacatcc gactgccgtt gagcgcaata aaggtcagga tgatgtactc
    14581 cagagacatg gcgtaaacgc cctgaagtgc aaaaataacg acgctaatca cgttaataat
    14641 cacccacaac agccagtttt ccacatactt acgcgtcatc agaatcattg ccacgataga
    14701 taacaccatc atgcatgaat cccagaacgg gaaagcgtcc ggttgcagtt caggcatcac
    14761 aacctgtaat cctaatgctt gcatgatcat gactgccacg cgggtcaaaa atgcaaacac
    14821 cggattgata aataccgtca tcagaccaat cgaaacaacg caaaccgcca accagctgag
    14881 cgccttcggc aatggtaacc agcgaatttt caactccgcc tcgttctgac tggtttgtcg
    14941 cgaccacgca taccaaccgt aaatattcgc ggcaaagaaa aacacctgta atagcaggct
    15001 ggcatacagc tgaatctgaa agaaaataat gccaaacaag gtgacgttga tcaggccaaa
    15061 gaagtagttg ctgatcttct ccagactggc aaggccaata cacagcaacc cggcgatcgt
    15121 gcctaccgct tcgatccatg agagatcata accgcctgcc cctattggta tatgtaccag
    15181 gatattctgc acactaaaaa aatccatctt tcccccaaag cgtaacgtta cgccttatta
    15241 ttcgttatcc acgtagtgta gccgcaaaat ccagcatacg attgagcggc accagcgccc
    15301 tctctcgcag cctttcatca agaacctcgt gattgcttcc ttcctgttct aatgcctctg
    15361 cgatggactg aaggccattc atggccatcc acggacaatg cgcgcag
//
LOCUS       DADMKF010000095        15341 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.097, whole genome shotgun sequence.
ACCESSION   DADMKF010000095 DADMKF010000000
VERSION     DADMKF010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15341
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.097"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            1..72
                     /locus_tag="JNC24_002714"
                     /pseudo
     CDS             1..72
                     /locus_tag="JNC24_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707860.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
     gene            71..1015
                     /locus_tag="JNC24_002715"
                     /pseudo
     CDS             71..1015
                     /locus_tag="JNC24_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311040.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            1051..1737
                     /gene="mlrA"
                     /locus_tag="JNC24_002716"
     CDS             1051..1737
                     /gene="mlrA"
                     /locus_tag="JNC24_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MlrA"
                     /protein_id="HAY9159422.1"
                     /translation="MPVTLRAWQRRYGLLKPQRTDGGHRLFNDADIDRIREIKRWIDN
                     GVQVSKVKMLLSNENVDVQNGWRDQQETLLTYLQSGNLHSLRTWIKERGQDYPAQTLT
                     THLFIPLRRRLQCQQPTLQALLAILDGVLINYIAICLASARKKQGKDALVVGWNIQDT
                     TRLWLEGWIASQQGWRIDVLAHSLNQLRPELFEGRTLLVWCGENRTSAQQQQLTSWQE
                     QGHDIFPLGI"
     gene            1797..1904
                     /locus_tag="JNC24_002717"
     CDS             1797..1904
                     /locus_tag="JNC24_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HAY9159423.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(1885..2616)
                     /gene="yehW"
                     /locus_tag="JNC24_002718"
     CDS             complement(1885..2616)
                     /gene="yehW"
                     /locus_tag="JNC24_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HAY9159424.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPVLQATLAGLGAIDASVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(2621..3547)
                     /gene="yehX"
                     /locus_tag="JNC24_002719"
     CDS             complement(2621..3547)
                     /gene="yehX"
                     /locus_tag="JNC24_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HAY9159425.1"
                     /translation="MIEFGHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGVIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVRLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPCGTLHFQDLLEEA"
     gene            complement(3540..4697)
                     /gene="yehY"
                     /locus_tag="JNC24_002720"
     CDS             complement(3540..4697)
                     /gene="yehY"
                     /locus_tag="JNC24_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HAY9159426.1"
                     /translation="MTYLRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGRHLWQL
                     WPQTIWMLVGVGCAWLTACFIPAKKGSIFALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGSGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGTLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLTDALFDLLIALLKVKRND"
     gene            complement(4704..5621)
                     /gene="osmF"
                     /locus_tag="JNC24_002721"
     CDS             complement(4704..5621)
                     /gene="osmF"
                     /locus_tag="JNC24_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HAY9159427.1"
                     /translation="MPLSKVWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVVRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNAQ
                     QGYEKVKKLDAEQNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLKEGGTF
                     KLAASAEFIERADALPAFEKAYDFKLDQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPKGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWTK"
     gene            complement(5873..8170)
                     /gene="bglX"
                     /locus_tag="JNC24_002722"
     CDS             complement(5873..8170)
                     /gene="bglX"
                     /locus_tag="JNC24_002722"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HAY9159428.1"
                     /translation="MKWLCSVGIAVSLALQPALADDLFGNHPLTPEARDAFVTELLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSTMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMEELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNAV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQSQRDLITALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            8366..10081
                     /gene="dld"
                     /locus_tag="JNC24_002723"
     CDS             8366..10081
                     /gene="dld"
                     /locus_tag="JNC24_002723"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HAY9159429.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARINEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYIHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            complement(10120..11052)
                     /gene="pbpG"
                     /locus_tag="JNC24_002724"
     CDS             complement(10120..11052)
                     /gene="pbpG"
                     /locus_tag="JNC24_002724"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HAY9159430.1"
                     /translation="MPKFRVSLFSLALMLAVPLAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(11226..11813)
                     /locus_tag="JNC24_002725"
     CDS             complement(11226..11813)
                     /locus_tag="JNC24_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HAY9159431.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLALTVILWGYGYRLF"
     gene            11983..12561
                     /locus_tag="JNC24_002726"
     CDS             11983..12561
                     /locus_tag="JNC24_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HAY9159432.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDHQA"
     gene            complement(12691..13452)
                     /locus_tag="JNC24_002727"
     CDS             complement(12691..13452)
                     /locus_tag="JNC24_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAY9159433.1"
                     /translation="MAQVAIITASDSGIGKECALLLAQQGFDIGITWHSDEEGAKDTA
                     REVVSHGVRAEIVQLELGKLPEGAQALEKLIQRLGRIDVLVNNAGAMTKAPFLDMAFD
                     EWRKIFTVDVDGAFLCSQIAARQMVKQGQGGRIINITSVHEHTPLPDASAYTAAKHAL
                     GGLTKTMALELVRHKILVNAVAPGAIATPMNGMDDSDVKPDAEPSIPLRRFGATHEIA
                     SLVAWLCSEGANYTTGQSLIVDGGFMLANPQFNPE"
     gene            complement(13505..15031)
                     /gene="mdtQ"
                     /locus_tag="JNC24_002728"
     CDS             complement(13505..15031)
                     /gene="mdtQ"
                     /locus_tag="JNC24_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311052.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HAY9159434.1"
                     /translation="MLLMYCHAKLKNISQHTVISAHRFLPDYSPMNRDSFYPAIACFP
                     LLLMLAGCAPMHETRQALSQQTPAAQVDTALPTALKNGWPDSQWWLEYHDNQLTSLIN
                     NALQNAPDMQVAEQRIQLAEAQAKAVATQDGPQIDFSADMERQKMSAEGLMGPFALND
                     PAAGTTGPWYTNGTFGLTAGWHLDIWGKNRAEVTARLGTVKARAAEREQTRQLLAGSV
                     ARLYWEWQTQAALNTVLQQIEKEQNTIIATDRQLYQNGITSSVEGVETDINASKTRQQ
                     LNDVAGKMKIIEARLSALTNNQTKSLKLKPVALPKVASQLPDELGYSLLARRADLQAA
                     HWYVESSLSTIDAAKAAFYPDINLMAFLQQDALHLSDLFRHSAQQMGVTAGLTLPIFD
                     SGRLNANLNIAKAESNLSIASYNKAVVEAVNDVARAASQVQTLAEKNQHQAQIERDAL
                     RVVGLAQARFNAGIIVGSRVSEARIPALRERANGLLLQGQWLDASIQLTGALGGGYKR
                     "
     gene            15165..15248
                     /gene="yohP"
                     /locus_tag="JNC24_002729"
     CDS             15165..15248
                     /gene="yohP"
                     /locus_tag="JNC24_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohP"
                     /protein_id="HAY9159435.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
BASE COUNT         3746 a         4175 c         3836 g         3584 t
ORIGIN      
        1 aaaaatatca tcagatggct gcttaaaaaa agaacaaatg gtagtgtccg ctattgccag
       61 tacacctcag ttgccaatgt taggtgaaaa aagactggtt tcatctgtaa caaaagagga
      121 tttgcttttc gtaaggagag atctgttgac cggttatcaa aagctttcta acggaaaaat
      181 ttcttccata aaaggacgct cagtggtcac agtaaactac tatatgacaa ccatagctgg
      241 aatgtttcaa tttgcaacag ataatggtta tacatcagga aatccattta acggtctgac
      301 accattaaaa aagtccaaga tagaaccaga tcccctcacc cgtgacgaat ttattcgttt
      361 tattgaggct tgccgtcatc aacaaacaaa aaacctgtgg attatcgctg tatacacggg
      421 tattcgtcac ggggagctgg tatcactggc ctgggaagat atagacctta aagcgaggac
      481 tataaccatc cgccggaatt atacaaaact aggcgaattc actccaccaa aaactgatgc
      541 tggtaccgga agaacgattc atctagttca accagctatt gatgctctta aaagccaagc
      601 ggaaatgacc atgcttggaa aacagcattc tgtagaggta aagcagaggg aatatgggag
      661 aagtactgtg cataaatgca cttttgtttt tagtcctcag gtaataaaac agcggcagtt
      721 ttccggaccg cactataagg ttgactccat cagggagtca tggacaagta tcttaaaacg
      781 cgcaggtctg agacacagaa aatcgtacca atccaggcat acttatgcat gctggtcact
      841 tgccgctgga gctaatccta gttttatcgc aagccagatg ggccacacaa acgcacaaat
      901 ggtattcaat gtttacggag catggatgaa agacaacaat cacgaacaga tagaactcct
      961 taacaaaaga ctatctgaaa gtgtcccatg tatgccccat aagaaagtgg ggtaaaataa
     1021 aaacttgtaa aatcagttag tttaccctta atccctgtca cgttacgcgc gtggcagagg
     1081 cgttacgggt tgctgaaacc gcaacggaca gacggcggtc atcgactgtt caacgatgcc
     1141 gatattgacc ggatccgcga gatcaaacgc tggatcgaca acggcgtgca ggtcagcaaa
     1201 gttaaaatgc tgctcagtaa tgaaaatgtt gatgtgcaga acggctggcg cgatcagcaa
     1261 gaaacattac tgacctacct gcaaagcggc aatctgcata gcctgcgaac gtggatcaaa
     1321 gagcgcggtc aggattaccc cgcccagaca ctcaccacac atctgtttat tcctctgcgc
     1381 cgacggcttc agtgccaaca accgactctc caggcgctgc tggcgatcct cgacggcgta
     1441 ctgatcaact acatcgccat ttgtctggct tcggcacgta aaaaacaggg taaagatgcg
     1501 ctggtggttg gctggaatat tcaggatacc acccgtctgt ggctggaggg ctggattgcc
     1561 agtcaacaag gatggcgcat tgatgtcctc gcccactcgc tcaatcaact acgccctgaa
     1621 ctattcgaag gccgtacatt gctggtgtgg tgcggtgaaa atcgaacctc cgcccaacag
     1681 cagcaactca ccagttggca agaacaaggc catgatattt tcccactcgg catttaatga
     1741 ttcgttaaca aatgcgcttt actgtacaat cctttcgtta acataaggag tgcaatatgc
     1801 gcatagctaa aattggggtc atcgccctgt tcctgtttat ggcgttaggc ggaattggtg
     1861 gcgtcatgct cgcaggttat acctttattt tgcgtgctgg ctaagcgcct gcaccagcct
     1921 ttcaaacagg cggtctgcga tgatcgccgc cagtgccacc agtaacgccc cctggatcac
     1981 atacgcggta ttaaatccgc taagcccgat gatgatgggc gtacccagcg tgctggcccc
     2041 taccgttgag gcgatcgtcg ccgtaccaat gttgataatc accgaagttc gcacgcccgc
     2101 cagaatcacc ggagccgcca gcggtagctc gaccttacgc agtcgctgac cacgactcat
     2161 tcccatacct ttcgcaactt ctgtcacgct ggcatcaatc gctcccagcc cggcaagtgt
     2221 cgcctgcagg acgggcagca caccgtaaag gatcaaggcg ataatcgctg gttgcagacc
     2281 aaagccgatc accggaacgg caatcgccag cactgcgaca ggcggaaaag tctgtccaac
     2341 ggcggcaata gtttccacca gtgggcgaaa ttccgcgccc cacgggcgag tgacagcaat
     2401 tccggcacca gtgccaatga tcaccgcaaa caaactcgaa attcccacca gccagaaatg
     2461 agccagtgcc agagctgcaa aactttcttg ctgataaacg ggtcgtggca gttgtgggaa
     2521 caaggcagca aacagcggct ggctgtaagg cagccagaaa atcagtgcca caaacaaagc
     2581 aatgagccag aacagcggat cgcgcaacat cttcatacgc ttacgcctcc tccagcagat
     2641 cctgaaaatg cagcgtgccg caaggctggc cctgcgtgtt caccaccggc agcacctcgc
     2701 atccccgcgc gacaaacagg gagagcgcat cgcgtagcgt catctcttct gccagtgcct
     2761 caccttctgc ccgttcttcg cgacgcacgt aatccgccac actacgtaac gaaagcaggc
     2821 gcacacccag ttcgctgcgc ccaaaaaact ggcggacaaa atcgtttgcc ggacgagtca
     2881 gcatcgtcag cggattgccc tgctgcacca cttcaccgtg atccatcaat accagatgtt
     2941 ctgccagccg tagcgcctca tcaatatcat gagtgaccag cacaatggta cgccccagca
     3001 aacggtgaat gcgcgtcatc tcttgttgca acgcgccgcg cgttaccggg tccagtgcgc
     3061 caaaaggttc atccatcagt aagacttgcg gatcggcagc cagtgcgcgc gccactccca
     3121 cacgttgctg ctgaccaccg gaaagctgat gcggataacg ctcacgtaaa tttgactcca
     3181 gccccagtag cgccattaat tcgtcgatac gatcgtcaat cctcgcccgc gaccattttt
     3241 gtaattgcgg cacggtagcg atgttttgcg ccacgctcca gtgggggaac aggccaatag
     3301 attgaatggc atagcccatc cggcggcgca actccagtac tggcagcgag cgaatttctt
     3361 ctccggcaaa gcggatcact ccgctgtcat gctccaccag gcggttaatc attttcaggg
     3421 tggtggattt gccggagcca gatgtgccaa tcagcaccga aaaactccct tcctgaaaat
     3481 tgagattgag atcgttaacg gctttttgtg cgccgaacag tttgctgaca tggccaaatt
     3541 caatcattac gtttcacctt cagcagtgcg ataagcaaat cgaacagcgc gtcggtaagc
     3601 accgccagaa caattaccgg gatcaccccc agcaacacta aatcaatggc gctgcttagc
     3661 agcccctgga aaaccagcgc accaaaaccg cctgcgccga ttaacgccgc aatcaccgcc
     3721 atacctacag tttgcaccat caccacccgc aggctgcgca gaaataccgg taacgccagt
     3781 ggtaactgaa catgcaggaa tcgctgcgcc ccgctcatgc ccatcgctct ggcgctctcc
     3841 agcacatcgc gcgggatctg gttcaaaccg actaccacgc cgcgcaccag cggcagcaag
     3901 gcatagagca ccagcgcaat cagtgcgggt gtcattccgg ttcctgctat gccgagcgtc
     3961 cccagccacg gaaaggccgt aaccagcgcg gcaagcggcg caatcaacag gccaaagagc
     4021 gccaccgaag gcacggtctg aatgacattg agcagagaaa aaattgcccc ctgccgagca
     4081 gtggaaaagt agcaccagat gcccaacggc acaccaatca ctaacgcagg cagcaccgca
     4141 ccaaacagca acgtcagatg ttgtgccagc gcgtcgtcaa acacatcctg acggttggcg
     4201 tattctttca ttagtgagag atcgttaagc gtgccggagt acagcaacca cagcggaata
     4261 atggcaatct gcatatgcaa caaccagcgc cacagcggat gcgtggagat tcggcggatg
     4321 gcatcactac aggccagcaa tgccagcgcc gcggccagcc agaaaccact gccgaggctg
     4381 gtacgcgcca gcgcactgcc attttgcgcc agttgggtcg ccgcctttcc tgctccccac
     4441 accagcaata cgaagacgaa ttgcgccaga atgagtgcaa aaatgctgcc ttttttagcg
     4501 ggaataaaac aggctgtcag ccaggcgcaa ccaacgccca ccagcatcca gatcgtttgc
     4561 ggccacagtt gccagagatg acgcccttca cccgagacta aacgattagg cgcgtaactg
     4621 ataaacggca gcgccgctgc gattgccgtc agcaacagca gcagcgccag aacaggatta
     4681 atacgtagat aagtcacggg aaattacttc gtccaccctt tttgtttcag gtagtcggca
     4741 gccacttttt tggcatccag tccttccact gcaatgctgg cattcagttg ctgcaatgtt
     4801 tttgcatcga ggctggcgaa gactggctgt agccactgtg ccatttgcgg atactctttc
     4861 agcaccgact cacgcaccac tggtgcaggc gcgtagatag gttgcacgcc tttcggatcg
     4921 cttaaggttt gcagccccag cgccgcgacc ggaccgtcag tgccgtaagc cattgcggca
     4981 ttaacgccag aagtttgctg ggcagcggct ttaatcgtta ccgccgtgtc gccgccagcc
     5041 agtgacagta actgatcctg atcgagttta aagtcgtagg ctttttcaaa cgcgggtaac
     5101 gcatcggcgc gttcgataaa ctctgccgag gctgccagtt tgaaggtgcc gccctctttc
     5161 agataacgac tcaggtcggc aagcgaagtg agtttgtttt tctctgccac atcctgacgc
     5221 acggcgatgg tccaggtgtt attcgctggt gcgggcgtca gccagattaa cttgttttgc
     5281 tctgcatcga gttttttgac tttctcgtaa ccttgctgcg cgtttttcca tgccgcatcg
     5341 ttttcatctt taaagaagaa agcgccatta ccggtatatt ccggatagat atccagttca
     5401 ccggaagtaa tcgccccgcg caccacagga gtcgttccga gttgcacttt attcaccgtc
     5461 ggtacgccgt ggctttccag cacctgcaag atgatgttgc cgagtagcgc gccttcggta
     5521 tcgatttttg aaccgacttt aacgggggaa gccgcctgta gcggcaggct cacggctgcc
     5581 aacataacca gtgaacctgc ccagaccttt gagagtggca tgatgctttc ctcattcttt
     5641 tactgttgtt ttcagcgaat taagagaaaa gcatagttga taatgacaga gttagcctga
     5701 gcggaaggat tcagttgcag aatcagataa atacttaaag ggcatatttt gcccggcgtt
     5761 tcttgccgga tgcggcgtaa acgccttatc cggcctacaa aggtcgaaaa ttcagcaagt
     5821 taagatatga acatcaggca aagggcgcaa acgtcgcgcc cttactaaag cattacagca
     5881 actcaaactc gcctttctta acgcgtgcgg aatcagtgcc gataaagaca ttgaacttgc
     5941 caggttcggc gtcatatttc atctgttgat tccagaactt cagcgcctcg atatcaatcg
     6001 ggaagctgac agtctgagtt tcgcccggtt tcagggtgat tttctcaaag cctttcagct
     6061 gcttcactgg acggctcatg gaagccgtca catcctgcaa gtacatctgt actaccgtgg
     6121 ccccttcgcg tttaccggtg ttcgtcacct gcacgctggc ggtcactttg ccgtcacgct
     6181 tcatggtcgg cgcagaaagt ttcacatcag agacggtgaa agtggtatag ctcagaccat
     6241 agccgaacgg ataaagcgcc ccgttagctt catcaaaata acgcgaagtg tatttgttcg
     6301 gcttgtcggc attatacgga cgaccggtat tcagatggct gtagtacacc gggatctgcc
     6361 cgacagaacg cgggaaggac attggcagct tgccggacgg gttgtaatcg ccaaacaata
     6421 catcggcaat tgcattaccg ccttcagtcc ccgcaaacca ggtttccaga atcgcatcag
     6481 cctgctgatc ttctttcacc agcgccagcg gacgcccgtt catcagcacc agcaccagcg
     6541 gtttaccggt ggctttcagc gccgtaatca agtcacgttg gctttgcgga atagtgatat
     6601 cggtacggct ggaggcctcg tgcgccatcc cctgtgcttc accgaccaca gccaccacca
     6661 catcagattg tttcgccgtc tgcaccgctt catcaatcat ctcttgcggc gagcgcggat
     6721 cgactttgac cgcttcttca tactgattca ggaaatcgat aatgcctttg tcactggtaa
     6781 cgttcgcccc tttggcatac agcactttac cgttttcacc gacggcattt ttaatcccgg
     6841 tcagtacggt cacggattga tcggcaacac cggctgcgga ccagctgccc atcacgtcac
     6901 gtttactgtc cgccagtggc ccaaccaccg caatggtggc cgattttttc agcggtaacg
     6961 tttcgagacg gtttttcagc aacaccaggc tttcgcgcgc cacttcacgc gcttctttac
     7021 ggtgcaggcg gctttcggca ttggtatcca ccgggtcaga ctctttcggc cccaaatggc
     7081 tgtatgggtc gttaaacaat cccatatcat atttaacgtt cagtacatgg cgggcagcat
     7141 cgtccagctc ttccatcgtc actttgccgg atttgatcaa cccaggcaga tacttcgagt
     7201 aatactcgtc gctcatactc atgttgattc cggatttcag cgccacgcgc accgcatctt
     7261 ccgggtctgc cgccgtgcca tgtttaatca gctctttgat tgcaccgtga tcggaaacgg
     7321 tgatgccttt aaagccccac tggtcgcgca gaacatcttt cagcagccag gaatcggagg
     7381 tggctggcgt gccgttcagc gagttcagcg ccaccatcac cgcgccgctg cctgcgtcca
     7441 gccccgcttt gtacggcggc atataatcat taaacaggcg ctgcggactc atatcgacgg
     7501 tgttgtactc tttaccgcct tctaccgcgc cgtatgcggc aaagtgtttg acgctggtca
     7561 tcaccgagta gcgatctgcc gggcttttac cctgcatcgc ttccaccatg gttttaccca
     7621 ttgttgaggt gagatacgta tcttcgccaa aaccttcgga agcacgtccc cagcgcggat
     7681 cgcgcgagac atcgaccatc ggtgcccagg tcatattcag gccatcatct gccgcttcat
     7741 aagcagagac acgtccgacc gttttcactg catcgaggtt aaaagacgag gccagaccga
     7801 ggctaatcgg gaacaccgtg cgctgaccgt gcagcacgtc gtaagcaaag aaaagaggaa
     7861 ttttcaggcg gcttaattcc atcacctgat cctgcatggc gcggatatcc tgacgggtta
     7921 cggtgttgaa aatcgcccca acctgaccgt ctttgatcat ctcgcggatc gcctctttcg
     7981 ggttatccgg accgacgctg attaagcgca gctgaccaat tttctcatca actgtcattt
     8041 tcttaagcag ttcggtgacg aacgcatccc gcgcttcggg cgttaatgga tggttgccga
     8101 ataaatcatc cgccagtgct ggctgcaggg ccagactcac cgcgattcct actgaacata
     8161 gccatttcat atggattttt cttcctctgt tgcccgacga ggcagcgaaa atggtgcaaa
     8221 aaccgtagtt tgccataagc atgatggaga gagaaaaaga atgctcagtt tattgtctga
     8281 attttcaaaa tattcactcg ctgaattgtc atacaaggcg ctattctagt ttgtgatatt
     8341 ttttcgccac cacaaggagt ggaaaatgtc ttccatgaca acaactgata ataaagcctt
     8401 tttgaatgaa cttgcccgtc tggtcggtca ttcacacctg ctcaccgatc ccgcaaaaac
     8461 ggcccgctat cgcaagggct tccgttctgg tcagggcgac gcgctggctg tcgttttccc
     8521 tggctcacta ctggaattgt ggcgggtgct gaaagcctgt gtcaccgccg acaaaattat
     8581 tctgatgcag gctgccaata caggcctgac cgaaggatcg acgccaaacg gtaacgacta
     8641 tgatcgcgat atcgtgatca tcagcaccct gcgtctcgac aagctgcacg ttcttggcaa
     8701 gggcgaacag gtgctggcct atccgggcac cacgctctat tcgctggaaa aagccctcaa
     8761 accgctggga cgcgaaccgc actcagtgat tggatcatcg tgtataggcg catcggtcat
     8821 cggcggtatt tgtaacaact ccggcggctc gctggtgcaa cgtggcccgg cgtataccga
     8881 aatgtcgtta ttcgcgcgta taaatgaaga cggcaaactg acgctggtga accatctggg
     8941 gattgatctg ggcgaaacgc cggagcagat ccttagcaag ctggatgatg atcgcatcaa
     9001 agatgacgat gtgcgtcacg atggtcgtca cgcccacgat tatgactatg tccaccgcgt
     9061 tcgtgatatt gaagccgaca cgcccgcacg ttataacgcc gatcctgatc ggttatttga
     9121 atcttctggt tgcgccggga agctggcggt ctttgctgta cgtctagata ccttcgaagc
     9181 ggaaaaaaat cagcaggtgt tttatatcgg caccaaccag ccggaagtgc tgaccgaaat
     9241 ccgccgtcat attctggcta acttcgaaaa tctgccggtt gccggggaat atatacaccg
     9301 ggatatctac gatattgcgg aaaaatacgg caaagacacc ttcctgatga ttgataagtt
     9361 aggcaccgac aagatgccgt tcttctttaa tctcaaggga cgcaccgatg cgatgctgga
     9421 gaaagtgaaa ttcttccgtc cgcattttac tgaccgtgcg atgcaaaaat tcggtcacct
     9481 gttccccagc catttaccgc cgcgcatgaa aaactggcgc gataaatacg agcatcatct
     9541 gctgttaaaa atggcgggcg atggcgtggg cgaagccaaa tcgtggctgg tggattattt
     9601 caaacaggcc gaaggcgatt tctttgtctg tacgccggag gaaggcagca aagcgttttt
     9661 acaccgtttc gccgctgcgg gcgcagcaat tcgttatcag gcggtgcatt ccgatgaagt
     9721 cgaagacatt ctggcgttgg atatcgctct gcggcgtaac gacaccgagt ggtatgagca
     9781 tttaccgccg gagatcgaca gccagctggt gcacaagctc tattacggcc attttatgtg
     9841 ctatgtcttc catcaggatt acatagtgaa aaaaggcgtg gatgtgcatg cgttgaaaga
     9901 acagatgctg gaactgctac agcagcgtgg cgcgcaatac cctgccgagc ataacgtcgg
     9961 tcatttgtat aaagcaccgg agacgttgca gaagttttat cgcgagaacg atccgaccaa
    10021 cagtatgaat ccggggatcg gtaaaaccag taagcggaaa aactggcagg aagtggagta
    10081 aaaattacgg atggcatagt atcgccatcc gtcattcact taatcgttct gtgccgtctg
    10141 ccccgccgcc gccatttggg cggctttttg ttttttatag ctcaacgctg ctgctggaac
    10201 aggcatcact ttaccagttt caatccaggt acgcaggcgg ctggcatcgg caaaatgggt
    10261 atatttgcca aacgcatcca tcactaccag cgctaccggt ttattattga taaccgtacg
    10321 catcaccaga caatggcccg ccgcattggt aaaaccggtt ttggttaact gaatattcca
    10381 gttatcgcga tacaccagat ggttagtatt gcggaacggc agcgtatacg tcgggttaga
    10441 gaaggttgcc atatcttccc gggtagtact taactgcccg atcaacggat attgtttgct
    10501 ggcaatgagc agtttggtta agtcacgggc agttgaaacg ttatgcaccg acaatccggt
    10561 aggttcaaca aagcgcgtgt tgttcattcc gagcgatttc gctttcgcat tcattgcctt
    10621 aataaaggct ttgtaaccac cgggataatg gtgtgcaagg cttgccgccg cgcggttttc
    10681 tgaagacatc agcgccagca acagcatatc tttacggctg atttcgctat tcagtcgtac
    10741 gcgcgaatag acccctttca tctccggcgt ctggctgata tccactttta gtttttcatc
    10801 cagcggcagt cgtgcatcca gcacaaccat cgcggtcatt aatttgctga tagacgcaat
    10861 cggacgcacc agatccgggt ggttcgagta gatcactttg ttggtattca gatcaacaat
    10921 catcgcgcta ccggaggcaa tttcaggttg tgaagcggtg gtagcggctg ccgttttcgc
    10981 aacggcctgc ggtgccaaag gcacagccag catcagggcc aggctaaata aagaaactcg
    11041 aaatttcggc atgatgagca ttcagatagt ggttcacgcg cacaggttgc gcaccgccgg
    11101 agtaaggatt tactgaggct agcgacgcca tcataacgag caaaaagtgc gatcgtcaaa
    11161 ggagaatcgt gaggaaatgc tgcattgctg acatttacgc cagcaatgca acgtcaaaga
    11221 actttctaga acaaacgata accgtagccc catagtataa cggttagggc gagcagcact
    11281 tccagtacca gcacgccaat cgccagcgtc gaactagaga agctaaggcc ttcctctttg
    11341 ttgatattca ggaagctcgg aataccaagg taaagcaggt agccggtgta aaacagcgcc
    11401 accgtgccga ccagcgcaca caaccagacc agtggataaa gcgccaccag accgcttaaa
    11461 aacagcggag ttgcaacgta gcccgcgaag accatgcagt gcgcaagtga cggacgctgc
    11521 ggataattac gcgccatcca ccagatgacc cgccccatta ccgcaacccc agccagcata
    11581 acgccataaa acaggacagc cagcgccagt ccggtaaacc aggataactt caggatagtg
    11641 ccatcgccaa aattccagcc aatctgtgta gtgccaatga aggcgcaaat caccggaatc
    11701 gccgccatca gcaaaacgtg gtgggtgtaa tggtgagaaa tcgtttcgtt ttcgcgatta
    11761 atcacctgca tttcacgatc gggatgggaa aacagtcccc agacatggct cataccgccc
    11821 ccttgttgtg agttcatgaa cctgacagtt caagtataag tcagcttgtg attatttttt
    11881 gttcgctcat gtaatttgca ccgtctgatg tacgtccttt ttcagcgtga atgatttcca
    11941 gggtgtatgc ttacaggcaa ccaaagggag acagactggc ctatggatct caatacactc
    12001 atctcacaat atggttatgc cgcgctggtg atcggtagcc tggcggaagg tgaaaccgtg
    12061 actttgctgg gaggcgttgc ggcacatcag gggctattaa agttcccgct ggtggtactt
    12121 tctgtggcgc ttggcggcat gattggcgac caggtgcttt atctgtgcgg gcggcggttt
    12181 ggcggcaagc tgttacgccg tttctcgaaa catcaggata aaattgagcg ggcgcagaaa
    12241 cttatccaac gccatccgta tctgtttgtc attggtacgc gctttatgta tggctttcgg
    12301 gtgattggcc cgacgctgat tggtgccagc cagctgccgc cgaaaatctt tctgccgctg
    12361 aatattctcg gcgcatttgc ctgggcgttg atttttacca ctattggtta cgctggtggt
    12421 caggtgattg cgccgtggtt gcacaatctc gaccagcatt tgaagcactg ggtctggttg
    12481 attctggtcg tggttctggt ggtgggcgtg cgctggtggc tgaaacggcg cgggaagaaa
    12541 aagccggatc atcaggcgta aaaccattgc cctgggtaag gcgttcgcgc cgcatccgac
    12601 atccggtgca ccatgcctga tgcgacgctt gtgcgtctta tcatgcctac caaaatttta
    12661 cccattgaaa taaccacgca cctcactgcg ctattctggg ttgaactgcg gattcgccaa
    12721 cataaagccg ccatccacta tcaacgactg cccggtggtg taatttgcgc cctctgagca
    12781 cagccacgct accaggctgg caatctcatg cgttgcgcca aaacgccgca gaggaatcga
    12841 aggctccgcg tcgggcttca cgtcgctgtc atccatgcca ttcattggcg tggcgatcgc
    12901 cccaggcgcg actgcgttca ccaaaatctt atgcctgacc agctccagcg ccatcgtttt
    12961 ggttaacccg ccgagcgcat gtttagcggc tgtgtaagcg ctggcatccg gcagcggcgt
    13021 atgttcatgt accgacgtaa tgttgatgat acgaccgccc tgcccttgtt tcaccatctg
    13081 gcgggccgca atttgtgagc ataaaaatgc accatcgaca tcaacggtaa aaatcttgcg
    13141 ccactcatcg aaagccatat caagaaacgg cgctttggtc attgcgcccg cattattcac
    13201 cagcacatca atacgcccca gccgttgaat gagtttctcc agcgcctgcg caccttctgg
    13261 cagtttaccg agctccagct gcacgatctc cgcacgtacg ccgtggctaa ctacctcacg
    13321 cgcggtatct tttgcccctt cttcatctga gtgccaggta atgccaatat caaacccctg
    13381 ctgcgccagt aataacgcgc actctttgcc gatccccgaa tcggaggcgg taataatcgc
    13441 aacctgtgcc atcaagttct ccacttaacg ctgaataaac gttaagtata gaaggcgcat
    13501 atcatcagcg tttgtaaccc ccgcccaacg caccagtgag ttgaatagaa gcatccagcc
    13561 actgcccttg caataacagg ccattggccc gctcacgcag cgcggggatt ctggcttcgc
    13621 tgacgcggga accaacaatg atgcccgcgt taaagcgcgc ctgcgcaaga cctaccacac
    13681 gcaaggcatc gcgctcaatt tgcgcctgat gctggttttt ctccgcaagt gtctgaacct
    13741 gactggctgc ccgcgccacg tcattcaccg cttcaaccac cgctttgttg tagctggcga
    13801 tagacaagtt gctttcggct tttgcgatat tgagattggc gttaagacga ccactatcga
    13861 aaatgggtag cgtcaggcct gccgtaacgc ccatttgctg cgcggaatga cggaacagat
    13921 cgcttaagtg caacgcatcc tgttgcagga aggccatcag gttgatgtca ggataaaatg
    13981 ccgcttttgc cgcatcaatg gtgcttagcg atgactcaac gtaccagtgc gccgcctgca
    14041 aatctgcccg ccgggccagt aaggagtacc ccagttcatc aggaagctgg cttgccactt
    14101 tcggcaacgc gaccggttta agcttcaatg actttgtctg gttatttgta agtgcgctta
    14161 accgtgcctc aataattttc attttccccg cgacatcgtt gagctgctgc cgggttttgc
    14221 tggcattaat atcggtttcc acaccttcaa ctgaagaagt aatcccgttc tgatatagct
    14281 ggcgatcggt cgcgataatg gtgttctgct ctttttctat ttgctgcaag accgtgttta
    14341 acgccgcctg ggtttgccac tcccagtaca ggcgggctac gctgccagcc agcaattggc
    14401 gggtttgctc gcgttccgcc gcccgtgctt taaccgtacc caggcgggca gtaacctccg
    14461 cccgattctt tccccagata tcgagatgcc agcccgccgt taagccaaaa gtaccgttgg
    14521 tgtaccacgg gccggtcgta cctgcggccg gatcgttcag agcaaacggc cccattaagc
    14581 cttctgccga cattttttgc cgctccatat ccgccgaaaa gtcgatctgc ggaccatcct
    14641 gagtggcaac tgccttcgcc tgggcttcag ctagctgaat gcgctgttca gccacctgca
    14701 tatccggtgc gttctgtagt gcattgttaa ttaatgaagt gagctgatta tcgtgatact
    14761 ccagccacca ttggctgtct ggccaaccat ttttcagcgc cgtgggtaat gcggtgtcaa
    14821 cttgtgcagc gggcgtttgc tggcttaacg cctggcgggt ttcatgcata ggcgcacacc
    14881 cggccagcat cagtaacagc ggaaaacagg cgatggctgg ataaaaggaa tcacgattca
    14941 tgggggaata atcaggtaag aaacggtgcg cggagattac cgtgtgttgc gatatatttt
    15001 ttagtttcgc gtggcaatac atcagtagca ataaaacgac atacccagaa aaatatagac
    15061 taagtaaatg atatcttccg atttatctta atcgtttatg gataacggca aagggcttcg
    15121 ttttttccta tacttattca gcactcacaa ataaaggaac gccaatgaaa attatactct
    15181 gggctgtatt gattattttc ctgattgggc tactggtggt gactggcgta tttaagatga
    15241 tattttaaaa ttaattaatg tcatcaggtc cgaaaataac gagaatattt cagtctctca
    15301 tcctgttgcg ctcctgtcat gtgcattgct tcatataatc a
//
LOCUS       DADMKF010000096        15235 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.098, whole genome shotgun sequence.
ACCESSION   DADMKF010000096 DADMKF010000000
VERSION     DADMKF010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15235
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.098"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            <1..749
                     /locus_tag="JNC24_002730"
     CDS             <1..749
                     /locus_tag="JNC24_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF945 family protein"
                     /protein_id="HAY9159436.1"
                     /translation="SKFNQINIINHGEDVAQIDAFVAKTMLDRVKDKDYINVNLTYEL
                     DKLTKGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAVHPELANDEVALQEV
                     NAALFKEYLPLLQKSEPTIKQPVRWKNALGELNANLDISIADPAKSSSSTNKDIKLLN
                     FDVKLPLNVVTETAKQLNLSEGMDAEKAQKQADKQISGMMTLGQMFQLITIDNNTASL
                     QLRYTPGKVVFNGQEMSEEEFMSRAGRFVH"
     gene            complement(790..1722)
                     /locus_tag="JNC24_002731"
     CDS             complement(790..1722)
                     /locus_tag="JNC24_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HAY9159437.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGKDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQNK"
     gene            complement(1842..2084)
                     /locus_tag="JNC24_002732"
     CDS             complement(1842..2084)
                     /locus_tag="JNC24_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9159438.1"
                     /translation="MSVPVYQIMGKGCHRQDENMSFSPLFVKIFCRSSGSLANRHVDN
                     FVHKLFTSLLIYYHAFYSMMFVFVFHAVKNKAIPNN"
     gene            2086..4872
                     /gene="polA"
                     /locus_tag="JNC24_002733"
     CDS             2086..4872
                     /gene="polA"
                     /locus_tag="JNC24_002733"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HAY9159439.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISRERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKEQGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            complement(5254..5886)
                     /locus_tag="JNC24_002734"
     CDS             complement(5254..5886)
                     /locus_tag="JNC24_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HAY9159440.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKAQLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            6468..6977
                     /gene="yihI"
                     /locus_tag="JNC24_002735"
     CDS             6468..6977
                     /gene="yihI"
                     /locus_tag="JNC24_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HAY9159441.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVAEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            7166..8539
                     /gene="hemN"
                     /locus_tag="JNC24_002736"
     CDS             7166..8539
                     /gene="hemN"
                     /locus_tag="JNC24_002736"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HAY9159442.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFQFNADAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPIEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(8729..8839)
                     /gene="yshB"
                     /locus_tag="JNC24_002737"
     CDS             complement(8729..8839)
                     /gene="yshB"
                     /locus_tag="JNC24_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HAY9159443.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(8951..10360)
                     /gene="glnG"
                     /locus_tag="JNC24_002738"
     CDS             complement(8951..10360)
                     /gene="glnG"
                     /locus_tag="JNC24_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HAY9159444.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNIQLNGPTTDIIGEAPAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            complement(10372..11421)
                     /gene="glnL"
                     /locus_tag="JNC24_002739"
     CDS             complement(10372..11421)
                     /gene="glnL"
                     /locus_tag="JNC24_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HAY9159445.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            complement(11595..13004)
                     /gene="glnA"
                     /locus_tag="JNC24_002740"
     CDS             complement(11595..13004)
                     /gene="glnA"
                     /locus_tag="JNC24_002740"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HAY9159446.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            13377..15200
                     /gene="typA"
                     /locus_tag="JNC24_002741"
     CDS             13377..15200
                     /gene="typA"
                     /locus_tag="JNC24_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HAY9159447.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
BASE COUNT         3965 a         3803 c         3914 g         3553 t
ORIGIN      
        1 agtcgaaatt taatcagatt aacattatca atcacgggga agacgttgcc caaatcgatg
       61 ctttcgttgc aaaaaccatg ctggatcgcg ttaaagacaa agattatatc aatgtcaatc
      121 tgacctacga acttgataag ttaacaaaag ggaatcagca actcggtagt ggtgagtggt
      181 cattgattgc tgaatctatt gatccctcag cagtgcgcca atttatcatc cagtataaca
      241 ttgctatgca gaagcagctt gctgtacacc ctgagttagc aaacgatgaa gttgctctgc
      301 aagaagtgaa tgctgcattg ttcaaagagt atttaccgtt attacaaaaa agtgagccga
      361 ccattaaaca accggtaaga tggaagaacg cactcggcga actaaatgcc aatctggata
      421 tcagtattgc cgacccagcc aaatcttcat catccacaaa caaagatatc aaattgctca
      481 attttgatgt gaagttaccg cttaatgtcg tcacagaaac cgcaaaacag cttaatttat
      541 ctgaaggaat ggatgcggaa aaagcgcaaa agcaggctga taaacaaatc agcgggatga
      601 tgacattggg tcagatgttt cagttaatca cgattgacaa caataccgcc tcgctgcagc
      661 tgcgttatac accgggtaaa gttgtgttta acggacagga gatgagcgaa gaagaattta
      721 tgtctcgtgc cggacgtttt gttcattaaa atactcactc gcctctgctc attcagaggc
      781 gagtaaaact tacttattct gacgctgtga tgacatgaga gacgtcagaa gccggtcttt
      841 ttcctgccac aatgagttca gccattgctg aaaatggcgc ttaaagcttt tgtcattgat
      901 gtagtcgcca tgtaactcat cggcaatagg ctgtaaatcc acatgtacga caatccgcgt
      961 cagtttaccg cttaacatat cgaagaacgg ctgacggtta ttgtccggat aacacagcgt
     1021 aacattaagc agtttatcga attgtttacc cagtacattt agcgccatcg caatgcctgc
     1081 cgcctttggt ggcaacaagt tttgaaaagt ggagtgggtt tgctgatgtt tttcttgcgt
     1141 gaaacgggag ccttcaacga aattgacaat ggtggtggga tgcaggcgaa acttttcaca
     1201 agaacgccga gtggtttcaa catctttacc acgtcgctcc ggatgacgta ataaataagc
     1261 gcgggaataa cgcttcataa acggcatatc cagcgcccag cacgccaggc caagaaatgg
     1321 cacccaggcc agctgttgct tgaggaaata tttattcatc ggaatgtgct tacgaaacag
     1381 cacgcacagt acgacaatat ctgcccaact acggtggtta caaataagca gataccagtt
     1441 cttcttactt aacccttcca gcccgtgaac ttcccattgc aggtgtgggt tcagatgcag
     1501 taataccgcc agaccttcac accagcaata catcataaaa tcacaaaaac gcgaaacctt
     1561 tcgccaaatg actggcacag gtagcaatag ttttacaatc ccggcaatga tgatcgggac
     1621 ggaacaaaaa atagtgacca aaatggtcaa tacgatactc agcaaaaggg ttatcgcagc
     1681 gagtattctc gtcataatga atttattcaa aaggttagcc atagacagtg cgctaaagaa
     1741 gcgcaaggcg cagattttag cagaaaacga cccaaataac ggatgatcct taaggagaaa
     1801 aataatccat atctatccac attagaaaaa atcccattat ctcaattatt agggatggct
     1861 ttatttttaa ctgcatgaaa aacaaagaca aacatcatgc tgtaaaaagc atgataataa
     1921 attaaaagcg atgtaaataa tttatgcaca aagttatcca catgacgatt tgcgagcgat
     1981 ccagaagatc tacaaaagat tttcacgaaa agcggtgaaa aactcatgtt ttcatcctgt
     2041 ctgtggcatc ctttacccat aatctgataa acaggcacgg acattatggt tcagatcccc
     2101 caaaatccac ttatccttgt agatggttca tcttatcttt atcgcgcata tcacgcgttt
     2161 cccccgctga ctaacagcgc aggcgagccg accggtgcga tgtatggtgt cctcaacatg
     2221 ctgcgcagtc tgatcatgca atataaaccg acgcatgcag cggtggtctt tgacgccaag
     2281 ggaaaaacct ttcgtgatga actgtttgaa cattacaaat cacatcgccc gccaatgccg
     2341 gacgatctgc gagcacaaat cgaacccctg cacgcgatgg ttaaagcgat gggactgccg
     2401 ctgctggcgg tttctggcgt agaagcggac gacgttatcg gtactctggc gcgcgaagcc
     2461 gaaaaagccg ggcgtccggt gctgatcagc actggcgata aagatatggc gcagctggtg
     2521 acgccaaata ttacgcttat caacaccatg acgaatacca tcctcggacc ggaagaggtg
     2581 gtgaataagt acggcgtgcc gccagaactg atcatcgatt tcctggcgct gatgggtgac
     2641 tcctccgata acattcctgg cgtaccgggc gtcggtgaaa aaaccgcgca ggcattgctg
     2701 caaggtcttg gcgggctgga tacgctgtat gccgagccag aaaaaattgc tgggttgagc
     2761 ttccgtggcg cgaaaacaat ggcagcgaag ctcgagcaaa acaaagaagt tgcttatctc
     2821 tcataccagc tggcgacgat taaaaccgac gttgaactgg agctgacctg tgaacaactg
     2881 gaagtgcagc aaccggcagc ggaagagttg ttggggctgt tcaaaaagta tgagttcaaa
     2941 cgctggactg ctgatgtcga agcgggcaaa tggttacagg ccaaaggggc aaaaccagcc
     3001 gcgaagccgc aggaaaccag tgttgcagac gaagcgccag aagtgacggc aacggtgatt
     3061 tcttatgaca actacgtcac catccttgat gaagaaacac tgaaagcgtg gattgcgaag
     3121 ctggaaaaag cgccggtatt tgcatttgat accgaaaccg acagccttga taacatctct
     3181 gctaacctgg tcgggctttc ttttgctatc gagccaggcg tagcggcata tattccggtt
     3241 gctcatgatt atcttgatgc gcccgatcaa atctctcgcg agcgtgcact cgagttgcta
     3301 aaaccgctgc tggaagatga aaaggcgctg aaggtcgggc aaaacctgaa atacgatcgc
     3361 ggtattctgg cgaattacgg cattgagctg cgtgggattg cgtttgatac catgctggag
     3421 tcctacattc tcaatagcgt tgccgggcgt cacgatatgg acagcctcgc ggaacgttgg
     3481 ttgaagcaca aaactatcac ttttgaagag attgcgggta aaggcaaaaa tcaactgacc
     3541 tttaaccaga ttgccctcga agaggccgga cgttacgccg ccgaagatgc ggatgtcacc
     3601 ttgcagttgc atctgaaaat gtggccagat ctgcaaaaac acaaagggcc gttgaacgtc
     3661 ttcgagaata tcgaaatgcc gctggtgccg gtgctttcac gcattgaacg taacggtgtg
     3721 aagatcgatc cgaaagtgct gcacaatcat tctgaagagc tcacccttcg tctggctgag
     3781 ctggaaaaga aagcgcatga aattgcaggt gaggaattta acctttcttc caccaagcag
     3841 ttacaaacca ttctgtttga aaaacagggc attaaaccgc tgaagaaaac gccgggtggc
     3901 gcgccgtcaa cgtcggaaga ggtactggaa gaactggcgc tggactatcc gttgccaaaa
     3961 gtgattctgg agtatcgtgg tctggcgaag ctgaaatcga cctacaccga caagctgccg
     4021 ctgatgatca acccgaaaac cgggcgtgtg catacctctt atcaccaggc agtaactgca
     4081 acgggacgtt tatcgtcaac cgatcctaac ctgcaaaaca ttccggtgcg taacgaagaa
     4141 ggtcgtcgta tccgccaggc gtttattgcg ccagaggatt atgtgattgt ctcagcggac
     4201 tactcgcaga ttgaactgcg cattatggcg catctttcgc gtgacaaagg cttgctgacc
     4261 gcattcgcgg aaggaaaaga tatccaccgg gcaacggcgg cagaagtgtt tggtttgcca
     4321 ctggaaaccg tcaccagcga gcaacgccgt agcgcgaaag cgatcaactt tggtctgatt
     4381 tatggcatga gtgctttcgg tctggcgcgg caattgaaca ttccacgtaa agaagcgcag
     4441 aagtacatgg acctttactt cgaacgctac cctggcgtgc tggagtatat ggaacgcacc
     4501 cgtgctcagg cgaaagagca gggctacgtt gaaacgctgg acggacgccg tctgtatctg
     4561 ccggatatca aatccagcaa tggtgctcgt cgtgcagcgg ctgaacgtgc agccattaac
     4621 gcgccaatgc agggaaccgc cgccgacatt atcaaacggg cgatgattgc cgttgatgcg
     4681 tggttacagg ctgagcaacc gcgtgtacgt atgatcatgc aggtacacga tgaactggta
     4741 tttgaagttc ataaagatga tgttgatgcc gtcgcgaagc agattcatca actgatggaa
     4801 aactgtaccc gtctggatgt gccgttgctg gtggaagtgg ggagtggcga aaactgggat
     4861 caggcgcact aagattcgcc tgaacatgcc cttttttcgt aagtaagcaa cataagctgt
     4921 cacgttttgt gatggctatt agaaattcct atgcaacaac tgaaaaaaaa ttacaaaaag
     4981 tgctttctga actgaacaaa aaagagtaaa gttagtcgcg tagggtacag aggtaagatg
     5041 ttctatcttt cagacctttt acttcacgta atcggatttg gctgaatatt ttagccgccc
     5101 cagtcagtaa tgactggggc gttttttatt gggcgaaaga aaagatccgt aatgcctgat
     5161 gcgctatgtt tatcaggcca acggtagaat tgtaatctat tgaatttacg ggccggatac
     5221 gccacatccg gcacaagcat taaggcaaga aaattattcg ccgtcctgcg tttcttctac
     5281 aggctgcatc tcgctaaacc aggtatccag tttctgccgc agcttgtcca cgccttgttt
     5341 cttcaacgaa gaaaacgttt caacctgcac atcaccgtta aacgccagta cagcttcacg
     5401 caccatattc aattgcgctt tacgtgcgcc gcttgccagt ttgtccgctt tggtcagcag
     5461 caccagaacg gcgatattgc tgtctaccgc ccactcaatc atctgctgat ccaaatcttt
     5521 cagcggatgg cgaatatcca ttagcaccac cagaccttgc aggctctgac gtttttcgag
     5581 gtattcgccg agcgcacgct gccatttgcg cttcatctct tccgggactt ccgcataacc
     5641 gtacccaggc aagtcaacca gacgcttgcc gtcagccact tcaaacaggt tgataagctg
     5701 ggtgcgccct ggggtttttg aggtacgagc caggcttttc tggttagtca gcgtgttcag
     5761 cgcgctggat ttacctgcgt tggaacggcc tgcaaaagcc acttcaattc cggtatcgga
     5821 aggtaggtgg cgaatatcag gcgcactcat cacaaaatgc gtctgttgat aattcaaatt
     5881 agtcaaagcg gtcgtctccg tcagtcaaag ctttgtggcg attatacctg aaccataata
     5941 aaaggcggat ttttcggcgt gagcgttgta agtaaaagcc atacgctttg tgagacattg
     6001 ccgatagtct ttatgcgaaa tagcagagaa aattctgcga tgcatgtcaa ataagctata
     6061 taaatcagtg aattgctttt atataaaact ctgaaaatgt gcaaatactg atggcggttg
     6121 attgtttgtt taaagcaaag gcgtaaagta gcacccatag agcgaggacg ctaacaggaa
     6181 caatgactca ggatgagggt caggagcgcc aggaggcgaa gacagaggat tgtcaggaag
     6241 acaaacgtcc ggagacgtaa ttaaacggaa atggaatcaa cacggattgt tccggctaaa
     6301 ggaaaaacag ggtgtgttgg cggcctgcaa ggattgtaag acccgttaag ggttatgagt
     6361 caggaaaaaa ggcgacagag taatctgtcg ccttttttct ttgcttgctt tctgttagat
     6421 tccgccgcaa atctatactg aataaaacgg ctaaaagacg aaccattatg aaaccatcat
     6481 cttcaaactc acgcagcaaa ggtcatgcaa aagcgcgtcg aaaaacacgc gaggagctgg
     6541 atcaggaagc tcgtgatcgc aagcgtcaga aaaaacgtcg tggtcatgcg ccgggcagcc
     6601 gcgcagcggg cggaaacacc acgtcaggca gcaaaggcca gaacgcacca aaagatccac
     6661 gtattggcag taaaacccct attccattgg gcgtggctga aaaagtcacc aaacagcaca
     6721 aaccgaagag tgagaaacct atgctttcac cgcaggcgga gttggaatta ctggaaacgg
     6781 atgagcgtct ggatgcgctg ctggaacgtc tggaagcagg cgaaaccctg agcgccgaag
     6841 agcaatcctg ggtggatgct aaactggatc gtattgatga gttgatgcag aaactcggcc
     6901 tctcttatga cgatgacgaa gaagaggaag aagacgagaa gcaagaagac atgatgcgtc
     6961 tgctgcgggg taactaacgg attgccaccg tgggccttcc cgttctgctg ataacccttc
     7021 cggttatatg ttatctggtg tggttattcg ttaaactaca gcggttgtcg cggcgacaaa
     7081 agtggctgcg caaccggctg atgacccgaa acgggcatcc gccggtacgc cgtagccgcc
     7141 agagacgcca tcggaaggag tgagcatgtc tgtacagcaa atcgactggg atctggccct
     7201 gatccagaaa tataactatt ccgggccacg atacacctcg tacccgaccg cgctggagtt
     7261 ttcagaagac ttcggcgaac aggcgttttt acaagccgtg gcgcgctatc ctgagcgtcc
     7321 attatctctc tacgtacata tcccgttctg ccataagctt tgttacttct gcggttgcaa
     7381 taagattgtt actcgccagc agcacaaggc cgatcagtat ctggacgcgc tggagcaaga
     7441 aatcgtccat cgtgcaccgc tgttcgccgg gcgtcacgtc agccaattgc actggggcgg
     7501 cggaacgccg acgtatctga ataaagcgca aatcagccgc ctgatgaagc tgctgcgcga
     7561 aaacttccag ttcaatgccg atgcggagat ttcgatcgaa gtcgatccgc gggaaatcga
     7621 actggatgta ctcgatcatt tacgcgccga aggctttaat cgcctgagca tgggcgtgca
     7681 ggacttcaac aaagaagtgc aacgtctggt taaccgcgag caggatgaag agttcatctt
     7741 tgcactgctt aaccatgcgc gtgagattgg ttttacctcc accaacatcg acctgattta
     7801 cggcctgccg aaacagacgc cggagagttt cgcctttacc ctgaaacgtg tggcggagct
     7861 gaaccccgat cgtctgagtg tctttaacta cgcgcatctg ccgaccattt ttgctgctca
     7921 gcgcaaaatc aaagatgctg acctgccgag tccgcagcaa aaactcgata tcctgcagga
     7981 aaccatcgcc ttcctgacgc aatcgggcta tcagtttatc ggtatggatc actttgcccg
     8041 tccggatgac gagctggcgg tggcccagcg tgaaggcgtg ctgcatcgta acttccaggg
     8101 ctacaccact cagggcgata ccgatctgct ggggatgggc gtttccgcca tcagcatgat
     8161 tggcgactgc tacgcgcaga accagaaaga gttgaagcag tactatcagc aagtggatga
     8221 acaaggcaat gctctgtggc gtggtattgc gctaacgcgt gatgactgta ttcgccgcga
     8281 tgtgattaag tcgctcatct gcaacttccg tctggattac gcccctattg agaaacagtg
     8341 ggatttgcac ttcgctgatt actttgcgga agatctcaag ctgctcgccc cgttagcaaa
     8401 agatgggctg gtggatgtgg atgagaaggg aatacaggtg acggcgaaag gtcgcttgct
     8461 gatccgcaac atttgcatgt gctttgatac ctatctgcgc cagaaagcgc ggatgcagca
     8521 gttctcacgg gtgatttaaa tagtgaatgg cgcttcgttt acaaagtaga aaacgaaagc
     8581 gccatcaatg tgctgagaac aagattgcct gatgcgctac gcttatcagg cctacaaaac
     8641 ctgttgaaaa tattgctttt gtaggccgga taaggcgttc acgccgcatc cggcatgaac
     8701 aaagagcact tcatcagcaa tctgaaactc aactaaacag cccaatcatt gcggcacaca
     8761 gcacggcagc aacggcagtt tgcggtgtgt ggctgtcaac tgcgccactc gataccagat
     8821 taattattga ttccagcata atgactctcc ccgttttccg ggcaagatca tactgaactt
     8881 atcggaacag taaagcgtaa aataccagca atttgcgctc aataatcaat ctttacacac
     8941 aagctgtgaa tcactccatc cccagctctt ttaacttacg cgtcagggtg ttgcggcccc
     9001 agccgagtag ccgcgccgct tcctgtttat gcccctgcgt atgtcgtaac gcggtcgtca
     9061 gtaacgtccg ctccagctct ggctgcgctt cggaaagcag attttgatga ccggaacgca
     9121 gcgctctgtc tgcccactgc gcaagaagcg ttgcccagct gtccggttgc atttgcgaag
     9181 tactctccgc aaccgttgat tcaaacagtt cgccgggcaa atcctgaatc aacacttcct
     9241 gcccggcggc catcaccgtt agccagcggc aggtgttttc cagctggcgc acgttgcctg
     9301 gccacgccag acgcgtcaga gcagcttcgg tttccggatg cagtaacttc gcttctacgc
     9361 ccagttcgcg cgcggcaacc tgtaaaaaat ggcgcgccag acggggaata tcttcccgac
     9421 gttcgcgcag cggcggcaga tgaacgcgga taacgttcag gcggtggaac agatcctcac
     9481 ggaacttacc ttcctgcact cgctgttcga gattctggtg agtggcagcg ataatacgca
     9541 catccacttt caccggcgca tagccgccaa cgcggtaaaa ctgaccgtct gccagcacgc
     9601 gcagcaaacg cgtctgcaca tccagcggca tatcaccaat ttcgtcgagg aataatgtac
     9661 cgccatcggc ctgttcaaaa cgcccctgac gaatggtatt cgcgccagta aacgcgcctt
     9721 tctcgtggcc aaacagttct gattcgatca aatcttttgg gatagctgcc atattcagcg
     9781 cgataaacgg cgctttggcg cgcggactgt ggcgatgcag ggcatgagcg accagttctt
     9841 taccggtgcc ggattcgccg ttaatcagca cgctaataga agaacgcgaa agccgaccga
     9901 taatacggaa cacgtcctgc atggctggcg cttcgccgat gatatcggtc gttgggccgt
     9961 taagctgaat attacgcggc tgctgctgtt cctggtaatg actgatagcg cgctcaacca
    10021 gcgccactgc ttcgtcgata tcaaacggtt tgggcagata atcaaacgcc ccttgttgat
    10081 aggcgctgac ggcagcatcc agatcggaat gtgcggtcat aatgatgacc ggaagcattg
    10141 gatggcgctg tttaatctgc ttgagcagcg ccagcccgtc cattcccggc atacggatat
    10201 ctgaaagcag cacatccggc gttttgctcg ccagcgcctc cagtacttcc gcgccgttct
    10261 caaatgtcgt acaggttaaa cccgctccag cgagcgcacg ttcaagcacc caacggatgg
    10321 aactatcgtc atcgactacc cagactatcc ctcgttgcat aaacgtcacc tttatttcct
    10381 gataggcagg taaaccgaga actcggtatg acctggccaa ctggtaaatt caattttgcc
    10441 tgaatgctga tcaatcaaat tacgggcgat ggataagcca agcccggtgc caccttcgcg
    10501 gccgctgacc atcgggtaaa acagcgtatc ctgcaaatga ggcggaatgc ccggcccgtt
    10561 atcttccaca tcaatccgcg ccgccagccg gtagcgctcg ccgtgtaagg tcagttgaaa
    10621 cgcggtgcgg gtacgcagaa tgatttcacc gccttccggc cccagcgcct gtagcgcatt
    10681 gcgcacaata ttcagcaaga cctgttcaat ttgatccggg tcgtgcgcca gttccggcag
    10741 gctggggtcg taatcacgaa tcaaccgcac gttgtccggc agttccatcg acaccagcgt
    10801 caccacgcgt tcagctactt tgtgaatact ttcggtaacg cgcgtaccgg gcagctgcgg
    10861 ccccaacaga cggtcgacca gatttcgcag ccggtccgcc tgttcgataa tcactttggt
    10921 atattcgagt agtgatgggt caggtaacgc tttgctgagc agctgcgccg cgccacgtaa
    10981 acctccaagc ggatttttaa tctcatgtgc caggccgcgc actaaatcac gggcagcaac
    11041 ctgctgggcg tgctgtagct gttcctgact taagcggcgc tggttatcca tcggagccat
    11101 ctccagcagg atcatgccgt ccggcatacg ctgggccgtc acagaaagga tatgcgagcg
    11161 cccgtcgatg accagcgtca cttcgttatc ggtaaaacct tgccccgcct ccagactttc
    11221 ttgcatcagc tcgatattta atgagaagta gctcaacagt tccggtaacg gtgtaccaaa
    11281 caatttgcgg gagctttggg cgagcagttg ttgcgcggcg gggttggcgt aatggatcgc
    11341 cagattgtca tcaattaaca aaatactgtt aatcagcgag ttgaggatct gcccagcatc
    11401 gggctgcgtg cctgttgcca taaagcagtc tcctgaacag gttgcaccat tttagtgcat
    11461 tatagctttt tacggataaa aagcgcgaag catcagagaa ttgacggaga aaaaagccca
    11521 tgcagagatg ggctgaaagt ttccacggca actaaatccc ggcgttgttg cgccgggtag
    11581 tacaacttca actattagac gctgtagtac agctcaaact ctaccggatg cggagtcata
    11641 cgcacgcggt catcttcttc gcgacgtaga gcgatgtacg catcaattgc ttcgtcagtg
    11701 aacacgccac cggctttcag gaactcgcgg tccagatcca gttcgttcag tgcttcttcc
    11761 agagagcctg caacctgtgg gatctctttc gcttcttctg gcggcaggtc atacaggttt
    11821 ttgtccatgg cttcgcccgg atggatcttg ttcttgatac catcaagacc ggccatcagc
    11881 agggcagcaa agcacaggta cgggttagct gccggatccg ggaaacgtac ttcgatacga
    11941 cgtgctttcg gagaagaaac caccggaata cggatagacg cagaacggtt acgcgcagag
    12001 taagccagca ttaccggtgc ttcatagccc gggaccagac gcttataaga gttggtggtc
    12061 gggtttgcca gggcgttaat cgctttagcg tgtttgatta cgccgccaat gtagtacagc
    12121 gcctgctcag acagacctgc gtatttgtcg cctgcgaaca ggttaacgcc gtttttagac
    12181 agagacatgt ggcagtgcat accggagccg ttatcaccga acatcggttt tggcataaag
    12241 gtcgcggttt taccgaagcg gtgcgctacg ttgtgcacaa catatttgta gatctgaatt
    12301 tcgtcagctt ttttggtcat agtattgaag cgggtagcca cttcgttctg accagcagtc
    12361 gctacttcgt ggtgatgggc ttcaaccacc agacccatct gttccatcac cagacacatt
    12421 tcagaacgaa tatcctgagc cgagtctacc ggtggaaccg ggaagtaacc gcctttcact
    12481 gccggacggt gacctttgtt accaccttcg tattgggtgg aggagttcca tgcgccttcg
    12541 atatcgtcga tagcaacgtg ggaaccggag atagatgatc cgaaacggat gtcatcgaac
    12601 aggaagaatt caggttctgg cccgaacagt acggtgtcgg caatgccagt ggaacgcagg
    12661 taatcttcgg cgcgcttcgc aatggagcgc gggtcacggt catagccttg cagggtgcca
    12721 ggttcaagga tgtcgcaacg gataatcagg gtggagtcgg cgaagaacgg gtcaatcact
    12781 gcggtggatg cgtctggcat cagcaccatg tcggactcgt taatgccttt ccagccgcca
    12841 atcgaggagc cgtcaaacat tttgccttct tcgaagaatt cagcattcac ctgatgagca
    12901 gggatagtga cgtgctgttc tttaccttta gtatcggtga agcgcaaatc aacaaacttc
    12961 acttcgtgct cgttcagcat cgtcagtacg tgttcagcgg acatacttta actctcctgg
    13021 attggtcatg gtcgtcgtgg taacgaaatc tgcaattatt ttggccgtgt cgccgtaaaa
    13081 aagataaagc gaaatctgtg ccaactttta aattgcccct aaaaggcgtt atcatgcgca
    13141 ccatcgtgca aaagggctgc accacgatgt gaatgttgca ccaatatagt gcttcaatgg
    13201 aaacattaag caccatgttg gtgcaatgac ctttggataa ccctttttat gctccgtgaa
    13261 agcgatcaca aagggactct gcaatacttg tttgcggagg atgtttgtga tcctgttttg
    13321 tagtgcgatt aatccgtgta caataacgcg ctatttctaa tgcctgaggc aaagttgtga
    13381 tcgaaaaatt gcgtaatatc gccatcatcg cgcacgtaga ccatggtaaa accaccctgg
    13441 tagacaagct gctccaacaa tccggtacgt tcgactctcg tgccgaaacc caagagcgcg
    13501 tgatggactc caacgatttg gagaaagagc gtgggattac catcctcgcg aaaaacaccg
    13561 ctatcaaatg gaatgattac cgtatcaaca tcgttgatac cccggggcac gccgacttcg
    13621 gtggtgaagt tgaacgtgta atgtccatgg tagactcagt gctgctggtg gttgacgcat
    13681 ttgacggccc gatgccgcaa acgcgcttcg taaccaaaaa agcgtttgct tacggcctga
    13741 agccgattgt tgttatcaac aaagttgacc gccctggcgc gcgtcctgat tgggttgtgg
    13801 atcaggtatt cgatctgttc gttaacctcg acgcgaccga cgagcagctg gacttcccga
    13861 tcgtttacgc ttctgcgctg aacggtatcg cgggtctgga ccacgaagat atggcggaag
    13921 acatgacccc gctgtaccag gcgattgttg accacgttcc tgcgccggac gttgaccttg
    13981 acggtccgtt ccagatgcag atttctcagc tcgattacaa cagctatgtt ggcgttatcg
    14041 gcattggccg catcaagcgc ggtaaagtga agccgaacca gcaggtcact atcatcgata
    14101 gcgaaggcaa aacccgcaac gcgaaagtcg gtaaagtgct gggccacctc ggtctggaac
    14161 gtatcgaaac cgatctggcg gaagctggcg atatcgtggc gatcacgggc cttggcgaac
    14221 tgaacatttc tgacaccgtt tgcgacacgc aaaacgttga agcgctgccg gcactctccg
    14281 ttgatgagcc gaccgtttct atgttcttct gcgttaacac ctcgccgttc tgcggtaaag
    14341 aaggtaagtt cgtaacgtct cgtcagatcc tggatcgtct gaacaaagaa ctggtacaca
    14401 acgttgcgct gcgcgtagaa gaaaccgaag acgccgatgc gttccgcgtt tctggtcgtg
    14461 gcgaactgca cctgtctgtt ctgatcgaaa acatgcgtcg tgaaggtttc gaactggcgg
    14521 tatcccgtcc aaaagtaatc ttccgtgaaa tcgacggtcg taaacaagag ccgtatgaaa
    14581 acgtgactct ggacgttgaa gaacagcatc agggttctgt aatgcaggcg ctgggcgaac
    14641 gtaaaggcga cctgaaaaac atgaatccag acggtaaagg ccgcgtacgt ctcgactacg
    14701 tgatcccaag ccgtggtctg attggcttcc gttctgagtt catgaccatg acttccggta
    14761 ctggtctgct gtactccacc ttcagccact acgacgacgt acgtccgggt gaagtgggtc
    14821 agcgtcagaa cggcgtactg atctctaacg gtcagggtaa agcggtcgcg ttcgcactgt
    14881 tcggtctgca ggaccgcggt aagctgttcc tcggtcacgg tgcagaagtt tacgaaggtc
    14941 agattatcgg tattcacagc cgctctaacg acctgactgt aaactgcctg accggtaaga
    15001 aactgaccaa catgcgtgct tccggtactg acgaagctgt tgttctggtt ccgcctatcc
    15061 gcatgactct ggaacaagct ctggagttca tcgatgatga cgaactggta gaagtgactc
    15121 cgacctctat ccgtattcgt aaacgtcacc tgacggaaaa cgatcgtcgt cgcgccaacc
    15181 gcgcaccgaa agacgattaa tttcgttttt tagttataaa aaacctgcca tcgct
//
LOCUS       DADMKF010000097        15084 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.099, whole genome shotgun sequence.
ACCESSION   DADMKF010000097 DADMKF010000000
VERSION     DADMKF010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15084
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.099"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            23..781
                     /locus_tag="JNC24_002742"
     CDS             23..781
                     /locus_tag="JNC24_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000115259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9159448.1"
                     /translation="MSVMELRDLLIALESRLHQKHKLNAAARLQYEQARNKVIKKMAE
                     NIPEILVDELKNADINRRVNTLELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLAR
                     AIIHAINNAEMRELALRITSLLDFLPLYDVDCQDNGNLEYDTYSQPEWKHNLFDHYLA
                     VLYRFKDESGKEQFSGTVVKTREATPGKEIEAITRRMLDFSPRLKKLAGVPCQVYVRT
                     VAANNAQPLTQDQCLRALHHLRVQSTSKTAPQAK"
     gene            complement(984..1337)
                     /locus_tag="JNC24_002743"
     CDS             complement(984..1337)
                     /locus_tag="JNC24_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HAY9159449.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(1475..3121)
                     /gene="groL"
                     /locus_tag="JNC24_002744"
     CDS             complement(1475..3121)
                     /gene="groL"
                     /locus_tag="JNC24_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HAY9159450.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(3165..3458)
                     /locus_tag="JNC24_002745"
     CDS             complement(3165..3458)
                     /locus_tag="JNC24_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004391824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HAY9159451.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            3734..4990
                     /gene="yjeH"
                     /locus_tag="JNC24_002746"
     CDS             3734..4990
                     /gene="yjeH"
                     /locus_tag="JNC24_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HAY9159452.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAAQVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTRGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPANIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LTCFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLAGLWLFLPKRKTPENGITT"
     gene            complement(5012..5482)
                     /gene="fxsA"
                     /locus_tag="JNC24_002747"
     CDS             complement(5012..5482)
                     /gene="fxsA"
                     /locus_tag="JNC24_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein FxsA"
                     /protein_id="HAY9159453.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDR"
     gene            5819..7255
                     /gene="aspA"
                     /locus_tag="JNC24_002748"
     CDS             5819..7255
                     /gene="aspA"
                     /locus_tag="JNC24_002748"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HAY9159454.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            7373..8674
                     /locus_tag="JNC24_002749"
     CDS             7373..8674
                     /locus_tag="JNC24_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter"
                     /protein_id="HAY9159455.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            8790..9128
                     /gene="cutA"
                     /locus_tag="JNC24_002750"
     CDS             8790..9128
                     /gene="cutA"
                     /locus_tag="JNC24_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HAY9159456.1"
                     /translation="MLDEKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            9104..10801
                     /locus_tag="JNC24_002751"
     CDS             9104..10801
                     /locus_tag="JNC24_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HAY9159457.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSHFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVPQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTHLT
                     LMSNRQQGGSPGGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGMLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPIFLLERVIGDIWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKKFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            10838..11413
                     /locus_tag="JNC24_002752"
     CDS             10838..11413
                     /locus_tag="JNC24_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAY9159458.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            11520..11595
                     /locus_tag="JNC24_002753"
     tRNA            11520..11595
                     /locus_tag="JNC24_002753"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11553..11555,aa:Phe,seq:gaa)
     gene            12212..13749
                     /gene="cadC"
                     /locus_tag="JNC24_002754"
                     /pseudo
     CDS             12212..13749
                     /gene="cadC"
                     /locus_tag="JNC24_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313142.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator CadC"
     gene            14114..>15084
                     /gene="cadB"
                     /locus_tag="JNC24_002755"
     CDS             14114..>15084
                     /gene="cadB"
                     /locus_tag="JNC24_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
                     /protein_id="HAY9159459.1"
                     /translation="MSSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWI
                     ISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPAFGFQTGVLYYHANWIGNLAIGI
                     TAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVM
                     TAIVGWHWFDAATYAANWNTADTTDGHAIIKSILLYLWAFVGVESAAVSTGMVKNPKR
                     TVPLATMLGTGLAGIVYIAATQVLSGMYPSSVMAASGAPFAISASTILGNWAAPLVSA
                     FTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPKKGLL"
BASE COUNT         3708 a         3764 c         3616 g         3996 t
ORIGIN      
        1 caataaagaa tcgttgttct ttatgtctgt catggaactg cgcgacctgc tgattgcgct
       61 ggaaagtcgt ctgcatcaaa agcataaact gaatgctgct gcacgccttc agtatgagca
      121 agctcgcaat aaagtcatta agaaaatggc agaaaacatc ccagaaattc tggttgatga
      181 gcttaaaaat gccgatatta atcgtcgggt taacacgctg gaacttaccg ataatcaagg
      241 cgaaaatctt accttcgtat taacgctaca tgacggtagc aaatgtgagt tagtcgtcaa
      301 tgagttgcag attgaaatgc tggcgcgagc aatcattcat gcaattaata atgcggaaat
      361 gcgtgaactg gcattacgta ttacctcact gttagatttc ctgccgttat acgacgttga
      421 ctgccaggac aatggcaatc tggaatatga cacctattcc caaccggaat ggaaacataa
      481 cctgttcgac cattatctgg cggtgcttta ccgctttaaa gatgaaagtg gcaaagaaca
      541 atttagcggc acagtggtga aaacgcgtga agcaacgccg ggtaaagaaa tagaggcgat
      601 cactcgccga atgcttgatt tcagcccacg actgaaaaaa ctcgcgggtg tgccatgtca
      661 ggtctatgtt cgcaccgtag cggcgaataa cgcccaaccg ctaacccagg atcaatgctt
      721 acgcgcgctg catcacttac gtgttcaatc caccagcaaa actgcaccac aggcgaaata
      781 atctcatctt tgctaatgcg aggctggagc catccagcct gaaatgactg ataacgtgat
      841 cgttggtcat gccggatgcg acgtaaacgc cttatccggc ctacaaattc tcaagcattc
      901 aatcagttgc aatgcctgct gtaggcctga taagcgaagc gcatcaggca ataatacgtt
      961 tgtcacccag cctctggatc acatcagttc gggcacttat aaacctgacc gataatctgg
     1021 ctatccgtcg ggacaaaact ggacaacatt ccctgcgacg ggctgctgat gccataaatc
     1081 acgttaccgc ccattgcagc cgcctggttg cgcagatcgt ttgctgcgcc gcgcatagaa
     1141 ccgccctctt ctccgtgttg cccggaaagc cagttgcttt gcttacctgt cgcagtacca
     1201 atcagctggc actctgcgcc tggctgctcg tccacaatgc gtacactctg accggcagca
     1261 ctcaattcgt tgctggagct acaacccgcc atcagtagcg cggcaccgac aatccctgct
     1321 aagtatttta cgtgcatgtt attccccata atcaatgagc tggacgccac tcgcgtcgtc
     1381 cgtgtctgaa tcttatacta gaaagatgac caaaagaaaa acccccagac atttctgccc
     1441 gggggtttgt ttatttctgc gaggtgcagg gcaattacat catgccgccc atgccaccca
     1501 tgccgcccat accgccagca gcgcctaagt cagctgcatc atttttcggc aggtcggtaa
     1561 ccatgcactc ggtggtgatc atcaggccag ccacagaagc cgcgtactgc agagcagaac
     1621 gggttacttt ggttgggtcc aggataccca tgtcgatcat gttgccgtat tcttcggttg
     1681 ctgcgttgta accgtagttg ccgtcgccgc ctttaacggt gttagcaaca acagacggtt
     1741 cttcgccgca gttcaggacg atctgacgca gcggagcttc cattgcacgc agtgcaactt
     1801 tgatacccac gttctggtct tcgttctgac cacgcaggtc agccagttta gacgctacgc
     1861 ggatcagcgc aacaccacca ccagcaacca cgccttcttc taccgcagca cgggtcgcgt
     1921 gcagggcgtc ttcaacgcgt gcttttttct ctttcatttc aacttcggta gcagcaccta
     1981 ctttgataac tgcaacgccg cctgccagtt tcgctacgcg ttcctgcagt ttttcacggt
     2041 cgtagtcaga agttgcttct tcaatctgct gacggatctg agcaacacgg ccctggattg
     2101 cagcttcttc acccacgcca tcgatgatag tggtggtgtc tttgttgatc acaacacgtt
     2161 tagcctgacc caggtcttcc agggttgctt tttccagttc cataccgatc tcttcagaga
     2221 tcacggtacc gccagtcagg gttgcgatat cctgcagcat agctttacga cgatcgccga
     2281 agcccggagc tttaactgca gcaactttca cgatgccacg catggtgtta acaaccagag
     2341 ttgccagcgc ttcgccttct acatcttcag cgatgatcag cagcggtttg cctgctttgg
     2401 caacggcttc cagaaccggc agcatttcgc ggatgttgga gattttcttg tcagccagca
     2461 ggatgaacgg gctttccagt tctactgcgc cagtttccgg cttgttgatg aagtaaggag
     2521 acaggtagcc acggtcgaac tgcatacctt caaccacgtc cagttcgtcc tgcagaccgg
     2581 taccgtcttc aacggtgata acgccttctt taccgacttt gtccatcgct tcagcgatca
     2641 gtttacctac ggtttcgtcg gagttagcgg agatggtacc aacctgagca atcgctttag
     2701 agtcagagca cggtacggac agcgctttca gttcttcaac tgcagcggta acggctttgt
     2761 cgataccacg tttcaggtcc atcgggttca tgcccgcagc aacagctttc agaccctcag
     2821 tgatgatagc ctgagccagt acagttgcag tggtggtacc gtcgcctgca gcgtcgttcg
     2881 ctttagaggc aacttctttc accatctgcg cacccatgtt ttcgaacttg tcttccagtt
     2941 cgatttcacg agcaacggaa acaccatctt tggtgatggt cggtgcaccg aaagatttat
     3001 ccagaactac gttacggcct ttcggaccga gggtaacttt cactgcatct gccagtacgt
     3061 ttacgccgcg cagcattttc acacgagcgt cgttaccgaa ttttacgtct ttagctgcca
     3121 ttatctttat tccttaaatt cgtatgttca gtgtcgtgcg aggattacgc ttcaacaatt
     3181 gccagaatgt cgctttcgga catgatcaac acttcttcat tgtcgatctt ctcagatttc
     3241 acaccgtagc catcgttgaa aataacgatg tcgccaactt tcacatccag cggcttcact
     3301 tcgccatttt caaggatacg gccattgccg acagccagca cttcgccgcg ggtggattta
     3361 gccgctgcag agccggtcag aacgatgccg ccagcagatt tagtttcaac ttctttacgc
     3421 ttgacgatca cgcgatcatg caatggacga atattcattg ataactctcc tttgagaaag
     3481 tccgtatctg ttatgggtga cgccggagct tacgtggttt cccggctggt gaccagagaa
     3541 atggggatga ggattcgccc cttcaagggg gaaaaagaaa aaaaattctg atcagcacaa
     3601 aatcgggtga aaaccctgat tcacctcaca tttcatcgca atttcttcat cgccgtcata
     3661 agcgaatctg attgtgctac catcgaaaat ctacgcagtt gcccaaaatt tgggcgcaat
     3721 cggatatcaa cccatgagtg gactcaaaca agaactgggg ctggcccagg gcattggcct
     3781 gctatcgacg tcattattag gcaccggcgt gtttgccgtt cctgcgttag ctgcacaggt
     3841 agcaggcaat aacagcctgt gggcgtggcc cgttttgatt atcttagtgt tcccgattgc
     3901 gattgtgttt gcgattctgg gtcgccacta tcccagcgca ggcggtgtcg cgcacttcgt
     3961 cggtatggcg tttggttcgc ggcttgagcg agtcaccggc tggttgtttt tatcggtcat
     4021 tcccgtgggt ttgcctgccg cgctacaaat tgccgccggg ttcggccagg caatgtttgg
     4081 ctggcatagc tggcaactgt tgttggcaga actcggtacg ctggcgctgg tgtggtatat
     4141 cggtactcgc ggtgccagtt ccagtgctaa tctacaaacc gttattgccg gacttatcgt
     4201 cgcgctgatt gtcgctatct ggtgggcggg cgatatcaaa cctgcgaata tccccttccc
     4261 ggcacctggt aatatcgaac ttaccgggtt atttgctgcg ttatcagtga tgttctggtg
     4321 ttttgtcggt ctggaagcat ttgcccatct tgcctcggaa tttaaaaatc cagagcgtga
     4381 ttttcctcgc gcattgatga ttggtctgct gctggcagga ttagtctact ggggctgtac
     4441 ggtagtcgtc ttacacttcg acgcctatgg tgaacaaatg gccgcggcag catcgcttcc
     4501 aaaaattgta gtgcaactgt tcggtgtagg agcgttatgg attgcctgcg taattggcta
     4561 tctgacctgc tttgccagtc tcaacattta tatacagagc ttcgcccgcc tggtctggtc
     4621 gcaggcgcaa cataatcctg accactacct ggcacgcctc tcttctcgcc atatcccgaa
     4681 taatgccctc aatgcggtgc tcggctgctg cgtggtgagc actttggtga ttcatgcttt
     4741 agagatcaat ctggacgctc ttattattta tgccaatggc atctttatta tgatttatct
     4801 gttatgcatg ctggcaggct gtaaattatt gcaaggacgt tatcgactac tggcggtggt
     4861 tggcgggctg ttatgcgttc tgttactggc aatggtcggc tggaaaagtc tctatgcgct
     4921 gattatgctg gcggggttat ggctgtttct gccaaaacga aaaacgccgg aaaatggcat
     4981 aaccacataa tccggcgttt cgacattaat cctagcgatc gtctttatga tcaaggcggt
     5041 cgcggtcatc atcctttcgc tggtactcac catcaaaagt attaccgcca ccggtcccgg
     5101 cgctaaaacc gccgcctggc atgcgagaaa agcgcaaatg tggcatcaac ttcactgtca
     5161 gatgcttttg caccggcggc aataaaagca gaagaccgag gaagtcggtg aaaaagcccg
     5221 gtaataaaag cagcaaaccg gcaataatca gcgaaacact tttaatcatc tccgccgcag
     5281 ggttttcacc cgccgccatt ttttgctgca tcagcacgaa attcttaaag ccctggttac
     5341 gtaccagtga cataccgata actgacgtga atataaccag cacgagggtc agcaataccc
     5401 ccaatacatg ggcaacctga ataaagattg aaatctcaat atagacataa aggaaaatgg
     5461 caataaaagg taaccagcgc aaaggtttct cctgtaatag cagccggtta aacccggcta
     5521 cctgaatggg ttgcgaatcg cgttcagctt atattgtggt cattagcaaa atttcaagat
     5581 gtttgcgcaa ctatttttgg tagtaatccc aaagcggtga tctatttcac aaattaataa
     5641 ttaaggggta aaaaccgaca cttaaagtga tccagattac ggtagaaatc ctcaagcagc
     5701 atatgatctc gggtattcgg tcgatgcagg ggataatcgt cggtcgaaaa acattcgaaa
     5761 ccacatatat tctgtgtgtt taaagcaaat cattggcagc ttgaaaaaga aggttcacat
     5821 gtcaaacaac attcgtatcg aagaagatct gttgggtacc agggaagttc cagctgatgc
     5881 ctactatggt gttcacactc tgagagcgat tgaaaacttc tatatcagca acaacaaaat
     5941 cagtgatatt cctgaatttg ttcgcggtat ggtaatggtt aaaaaagccg cagctatggc
     6001 aaacaaagag ctgcaaacca ttcctaaaag tgtagcgaat gccatcattg ccgcatgtga
     6061 tgaagtcctg aacaacggaa aatgcatgga tcagttcccg gtagacgtct accagggcgg
     6121 cgcaggtact tccgtaaaca tgaacaccaa cgaagtgctg gccaatatcg gtctggaact
     6181 gatgggtcac cagaaaggtg aatatcagta cctgaacccg aacgaccatg ttaacaaatg
     6241 tcagtccact aacgacgcct acccgaccgg tttccgtatc gcagtttact cttccctgat
     6301 taagctggta gatgcgatta accaactgcg tgaaggcttt gaacgtaaag ctgtcgaatt
     6361 ccaggacatc ctgaaaatgg gtcgtaccca gctgcaggac gcagtaccga tgaccctcgg
     6421 tcaggaattc cgcgctttca gcatcctgct gaaagaagaa gtgaaaaaca tccaacgtac
     6481 cgctgaactg ctgctggaag ttaaccttgg cgcaacagca atcggtactg gtctgaacac
     6541 gccgaaagag tactctccgc tggcagtgaa aaaactggct gaagtcactg gcttcccatg
     6601 cgtaccggct gaagacctga tcgaagcgac ctctgactgc ggcgcttatg ttatggttca
     6661 cggcgcgctg aaacgcctgg ctgtgaagat gtccaaaatc tgtaacgacc tgcgcttgct
     6721 ctcttctggt ccacgtgccg gcctgaacga gatcaacctg ccggaactgc aggcgggctc
     6781 ttccatcatg ccagctaaag taaacccggt tgttccggaa gtggttaacc aggtatgctt
     6841 caaagtcatc ggtaacgaca ccactgttac catggcagca gaagcaggtc agctgcagtt
     6901 gaacgttatg gagccggtca ttggccaggc tatgttcgaa tccgttcaca ttctgaccaa
     6961 cgcttgctac aacctgctgg aaaaatgcat taacggcatc actgctaaca aagaagtgtg
     7021 cgaaggttac gtttacaact ctatcggtat cgttacttac ctgaacccgt tcatcggtca
     7081 ccacaacggt gacatcgtgg gtaaaatctg tgccgaaacc ggtaagagtg tacgtgaagt
     7141 cgttctggaa cgcggtctgt tgactgaagc ggaacttgac gatattttct ccgtacagaa
     7201 tctgatgcac ccggcttaca aagcaaaacg ctatactgat gaaagcgaac agtaatcgta
     7261 cagggtagta caaataaaga aggcacgtca gatgacgtgc cttttttctt gtgagcagta
     7321 acttaaaaat aacaatctaa tatcaacttg ttaaaaaaca aggaaggcta atatgctagt
     7381 tgtagaactc atcatagttt tgctggcgat cttcttgggc gccagattgg ggggaatagg
     7441 tattggtttt gcaggcggat tgggggtgct ggttcttgcc gctattggcg ttaaacccgg
     7501 taacatcccg ttcgatgtca tttccattat catggcggtt atcgccgcta tttctgccat
     7561 gcaggttgct ggcggtctgg actatctggt tcatcagaca gaaaagctgc tgcgccgtaa
     7621 cccgaaatac atcacaatcc tcgcaccgat cgtgacctat ttcctgacaa tctttgctgg
     7681 tactggcaac atctctctgg cgacactgcc agttatcgct gaagttgcga aggaacaagg
     7741 cgttaaacct tgccgtccgc tgtctactgc agtggtatcc gcgcagattg cgatcaccgc
     7801 atcgccaatc tcagcggcag tggtttacat gtcttccgtg atggaaggtc atggcatcag
     7861 ctacctccat ctgctctccg tggtcatccc gtccaccctg ctggcggttc tggtgatgtc
     7921 cttcctggtc actatgctgt tcaactccaa actctctgac gatccgattt atcgcaagcg
     7981 tctggaagag ggcctggttg aactgcgcgg tgaaaagcag attgaaatca aatccggtgc
     8041 aaaaacgtcc gtctggctgt tcctgctggg cgtagttggc gtggttatct atgcaatcat
     8101 caacagccca agcatgggtc tggttgaaaa accgctgatg aacaccacca acgcaatcct
     8161 gatcatcatg ctcagcgttg caactctgac caccgttatc tgtaaagtcg ataccgacaa
     8221 catcctcaac tccagcacct tcaaagcagg tatgagcgcc tgtatttgta tcctgggtgt
     8281 tgcgtggctg ggcgatactt tcgtttccaa caacatcgac tggatcaaag ataccgctgg
     8341 tgaagtgatt cagggtcatc cgtggctgct ggccgtcatc ttcttctttg cttctgctct
     8401 gctgtactct caggctgcaa ccgcaaaagc actgatgccg atggctctgg cactgaacgt
     8461 ttctccgctg accgctgttg cttctttcgc tgcggtgtct ggtctgttca ttctgccgac
     8521 ctatccgacg ctggttgctg cggtacagat ggatgacacg ggtacaaccc gtatcggtaa
     8581 attcgtcttc aaccatccgt tcttcatccc gggtactctg ggtgttgccc tggccgtttg
     8641 cttcggcttc gtgctgggta gcttcatgct ataatgacca atcgcggggc gttcacgccc
     8701 cgctttcttt cccgccgact aacatccttc ccccgtccgt tgtatagtga cctctctctt
     8761 gcggttccat ctgttcttgc gaggtgttta tgcttgatga aaaaagttcg aataccgcgt
     8821 ctgtcgtggt gctatgtacc gcaccggatg aagcgacagc ccaggattta gccgccaaag
     8881 tgctggcgga aaaactggcg gcctgcgcga ccttgatccc cggcgctacc tctctctatt
     8941 actgggaagg taagctggag caagaatacg aagtgcagat gattttaaaa actaccgtat
     9001 ctcaccagca ggcactgctg gaatgcctga agtctcatca tccatatcaa accccggaac
     9061 ttctggtttt acctgttaca cacggagaca cagattacct ctcatggctc aacgcatctt
     9121 tacgctgatc ctgctacttt gcagcacttc cgtttttgcc ggattattcg acgcgccggg
     9181 acgttcacat tttgtccccg cggatcaagc ctttgctttt gattttcagc aaaaccaaca
     9241 tgacctgaat ctgacctggc agatcaaaga cggttactac ctctaccgta aacagatccg
     9301 cattacgccg gaacacgcga aaattgccga cgtgcagctg ccgcaaggcg tctggcatga
     9361 agatgagttt tacggcaaaa gcgagattta ccgcgatcgg ctgacgcttc ccgtaaccat
     9421 caaccaggcg agtgcgggag cgacgttaac tgtcacctac cagggctgtg ctgatgccgg
     9481 tttctgttat ccgccagaaa ccaaaaccgt tccgttaagc gaagtggtcg ccaacaacgc
     9541 agcgtcacag cctgtgtctg ttccgcagca agagcagcac accgcgcaat tgcccttttc
     9601 cgcgctctgg gcgttgttga tcggtattgg tatcgccttt acgccatgcg tgctgccaat
     9661 gtacccactg atttctggca tcgtgctggg tggtaaacag cggctctcca ctgccagagc
     9721 attgttgctg acctttattt atgtgcaggg gatggcactg acttacacgg cgctgggtct
     9781 ggtagttgcc gccgcagggt tacagttcca ggcggcgcta cagcacccat acgtgctcat
     9841 tggcctcgcc atcgtcttta ccttgctggc gatgtcaatg tttggcttgt ttactctaca
     9901 actcccctct tcgctgcaaa cacatctcac attgatgagc aatcgccaac agggcggctc
     9961 acctggcggt gtgtttgtta tgggggcgat tgccggactg atctgttcac catgcaccac
    10021 cgcaccgctt agcgcgattc tgctgtatat cgcccaaagc gggaacatgt ggctgggcgg
    10081 cggcatgctt tatctctatg cgttgggcat gggcctgccg ctgatgctaa ttaccgtctt
    10141 tggtaaccgc ttgctgccga aaagcggccc gtggatggaa caagtcaaaa ccgcgtttgg
    10201 ttttgtgatc ctcgcactgc cgatcttcct gctggagcga gtgattggtg atatatgggg
    10261 attacgcttg tggtcggcgc tgggtgtcgc attctttggc tgggccttta tcaccagcct
    10321 acaggccaaa cgcggctgga tgcgcgtggt gcaaataatc ctgctggcag cggcattggt
    10381 tagcgtgcgc ccacttcagg attgggcatt tggtgcgacg cataccgcgc aaactcagac
    10441 gcatctcaac tttacacaaa tcaaaacggt agatgagtta aatcaggcgc tcgttgaagc
    10501 caaaggcaaa ccggtgatgt tagatcttta tgccgactgg tgcgtcgcct gtaaaaagtt
    10561 tgagaaatac accttcagcg acccgcaggt gcaaaaagcg ttagcagaca cggtcttact
    10621 tcaggccaac gtcactgcta acgacgcaca agatgtggcg ctgttaaagc atcttaatgt
    10681 ccttggacta ccgacaattc tcttttttga cggacaaggc caggagcatc cacaagcacg
    10741 cgtcacgggc tttatggatg ctgaaacctt cagcgcacat ttgcgcgatc gccaaccgtg
    10801 aacgacactt gcagtgggaa agacggagga aaatagcgtg caacgtgaag atgtactggg
    10861 agaagccctg aaattattag aattacaagg gattgccaac accacgctgg agatggttgc
    10921 tgaacgtgtg gattatccac tggacgagct acgccgcttc tggccagaca aagaggcaat
    10981 cctctacgat gcgctgcgtt atcttagcca acagatagat gtctggcgtc gtcagctgat
    11041 gcttgacgaa acgcaaaccg ccgaacaaaa gctgctggca cgttatcagg cgttatcgga
    11101 gtgcgttaaa aacaaccgct atccgggctg tctatttatc gctgcctgta cgttttatcc
    11161 cgaccctggc caccctattc atcaactggc cgatcagcaa aaaagcgcgg cctacgattt
    11221 cacccacgaa ctgttaacca cgctggaagt tgacgatccg gcgatggtag caaagcagat
    11281 ggaactggtg ctggaaggct gtttaagccg aatgctggtg aatcgtagcc aggcggatgt
    11341 cgacaccgca catcggctgg cggaagatat cctgcgcttc gcccgctgcc gccagggtgg
    11401 tgcactgacc tgacagaaac acagaaaaga agcgatttgc cgcaatctta agcagttgaa
    11461 tcgcttttac tgaaattagg ttgacgagat gtgcagatta cggtttaatg cgccccgttg
    11521 cccggatagc tcagtcggta gagcagggga ttgaaaatcc ccgtgtcctt ggttcgattc
    11581 cgagtccggg caccaaattc atataaacgg acctccacgg aggtccgttt ttcgtttcag
    11641 gacgccacga tttaagcgtt ctgcctccaa atcaattcta ccgaactcaa ccagattctc
    11701 cccacatcac cagcattttg cgggtatatc ccaattcggg aaaatttgtt cctgagcgat
    11761 agcactgact gacgtgaaat gtcgtgcggc cccgtgatgt tgttgaacgt caaatgacgt
    11821 catcaggagc gtaacgcatc cataaagcac aacaccggtc tgaacggcga ctgatgagat
    11881 tttctgaatg agaacaaaga gaaatgtatc agtccgcttg ctcatgcaaa gactaacaat
    11941 ccattaaaat agtaagcgct ccggacaatt ttccatggat tattttctac acatttttct
    12001 ttggcaaaga tgatgaattt tgatggtaag gaatattact tctggttctc agtaaaatcc
    12061 tttcgtaata ctatgtaatc aagaagttta tggctggtaa aaataacgtc ttgcattcac
    12121 caataacatg taaataaacc catctattga tggtaaaaat aggtcatggc aattatcatt
    12181 gcaccattcc cttttcgaat gagtttctat tatgcaacaa cctgtagttc gcgttggcga
    12241 atggcttgtt actccgtcca taaaccaaat tagccgcaat gggcgtcaac ttacccttga
    12301 gccgagatta attgatcttc tggttttctt tgctcaacac agtggcgaag tacttagcag
    12361 ggatgaactt atcgataatg tctggaagag aagtattgtc accaatcacg ttgtgacgca
    12421 gagtatctca gaactacgta agtcattaaa agataatgat gaagatagtc ctgtctatat
    12481 cgctactgta ccaaagcgcg gctataaatg aatggtgccg gttatctggt acagcgaaga
    12541 agagggagag gaaataatgc tatcttcgcc tccccctata ccagaggcgg ttcctgacac
    12601 aaattctccc tcccacagtc ttaccattca aaacaccgca acgccacctg aacaatcccc
    12661 agttaaaagc aaacgattca ctaccttttg ggtatggttt ttttcctgtt gtcgttaggt
    12721 atctgtgtag cactggtagc gttttcaact cttgatacac gtcttcctat gagcaaatcg
    12781 cgtattttgc tcaatccacg cgatattgac attaatatgg taaataaaag ttgtaacagc
    12841 tggagttccc cgtatcagct ctcttacgcg ataggcgtgg gtgatttggt ggcgacatca
    12901 cttaacacct tctccacctt tatggtgcat gacaaaatca actacaacat tgatgaaccg
    12961 agcagttccg gtaaaacatt atctattgcc tttgttaatc agcgccaata ccgtgctcaa
    13021 caatgcttta tgtcggtaaa attagtagac aatgcagatg gttcaaccat gctggataaa
    13081 cgttatgtca tcactaacgg taatcagctg gcgattcaaa atgatttact ggagagttta
    13141 tcaaaagcgt taaaccaacc gtggccacaa cgaatgcagg agacgctcca gcaaattttg
    13201 cctcatcgtg gtgcgttatt aactaatttt tatcaggctc atgattattt actgcatggc
    13261 gatgacaaat cattgaatcg tgccagtgaa ttattaggtg agattgttca atcatcccca
    13321 gaatttacct acgcgagagc agaaaaagca ttagttgata tcgtgcgcca ttctcaacac
    13381 cctttagatg aaaaacaatt agcagcactg aacacagaaa tagacaacat tgttacactg
    13441 ccggaattga acaacctgtc cattatatat caaataaaag cggtcagtgc tctggtaaaa
    13501 ggtaaaatag atgagtctta ccaggcgata aatactggca ttgatcttga aatgtcctgg
    13561 ctaaattatg tgttgcttgg caaggtttat gaaatgaagg ggatgaaccg ggaagcagct
    13621 gatgcatatc tcaccgcctt taatttacgc ccaggggcaa acacccttta ctggattgaa
    13681 aatggtattt tccagacttc tgttccttat gttgtacctt atctcgacaa atttctcgct
    13741 tcagaataag taactccggg ttgatttatg ctctggaata tttgttgttg agtttttgta
    13801 tgttcccatt ggtataatat gttgcggcaa tttatttgcc gcataatttt tattacataa
    13861 atttaaccag agaatgtcat gcaatccatt gtaaacattg aatgtttatc ttttcatgat
    13921 atcaacttgc gatcctgatg tgttaataaa aaacctcaag ttctcactta cagaaacttt
    13981 tgtgttattt cacctaatct ttaggattaa tccttttttc gtgagtaatc ttatcgccag
    14041 tttggtctgg tcaggaaata gttatacatc atgacccgga ctccaaattc aaaaatgaaa
    14101 ttaggagaag agcatgagtt ctgccaagaa gatcgggcta tttgcctgta ccggtgttgt
    14161 tgccggtaat atgatgggga gcggtattgc attattacct gcgaacctag caagtatcgg
    14221 tggtattgct atctggggtt ggattatctc tattattggt gcaatgtcgc tggcgtatgt
    14281 atatgcccga ctggcaacaa aaaacccgca acaaggtggc ccaattgctt atgccggaga
    14341 aatttctcct gcatttggtt ttcagacagg tgttctttat taccatgcta actggattgg
    14401 taacctagcg attggtatta ccgctgtatc ttatctttcc accttcttcc cagtattaaa
    14461 tgatcctgtt ccggcgggta tcgcctgtat tgctatcgtc tgggtattta cctttgtaaa
    14521 tatgctcggc ggtacctggg taagccgttt aaccactatt ggtctggtgc tggttcttat
    14581 tcctgtggtg atgactgcta ttgttggctg gcattggttt gatgcggcaa cttatgcagc
    14641 taactggaat actgcggata ccactgatgg tcatgcgatc attaaaagta ttctgctcta
    14701 cctgtgggcc ttcgtgggtg ttgagtccgc agcagtaagt actggtatgg ttaaaaaccc
    14761 gaaacgtacc gttccgctgg caaccatgct gggtactggt ttagcaggta ttgtttacat
    14821 cgctgcgact caggtgcttt ccggtatgta tccgtcttct gtaatggcgg cttccggtgc
    14881 tccgtttgca atcagtgctt caactatcct cggtaactgg gctgcgccgc tggtttctgc
    14941 attcaccgcc tttgcgtgcc tgacttctct gggctcctgg atgatgttgg taggccaggc
    15001 aggtgtacgt gccgctaacg acggtaactt cccgaaagtt tatggtgaag tcgacagcaa
    15061 cggtattccg aaaaaaggtc tgct
//
LOCUS       DADMKF010000098        14987 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.100, whole genome shotgun sequence.
ACCESSION   DADMKF010000098 DADMKF010000000
VERSION     DADMKF010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14987
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.100"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            <1..160
                     /locus_tag="JNC24_002756"
     CDS             <1..160
                     /locus_tag="JNC24_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="formate transporter"
                     /protein_id="HAY9159460.1"
                     /translation="WQLAHSSADNFPALTVSHFITANLLPVMLGNIIGGAVLVSMCYR
                     AIYLRQES"
     gene            complement(198..1259)
                     /locus_tag="JNC24_002757"
     CDS             complement(198..1259)
                     /locus_tag="JNC24_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAY9159461.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPALMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            1472..2935
                     /gene="bepA"
                     /locus_tag="JNC24_002758"
     CDS             1472..2935
                     /gene="bepA"
                     /locus_tag="JNC24_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HAY9159462.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSQQQARYDARIDQLRQLQERFKPYTKM"
     gene            2956..3315
                     /gene="arsC"
                     /locus_tag="JNC24_002759"
     CDS             2956..3315
                     /gene="arsC"
                     /locus_tag="JNC24_002759"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311384.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HAY9159463.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKKNGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIVVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(3453..4199)
                     /gene="hda"
                     /locus_tag="JNC24_002760"
     CDS             complement(3453..4199)
                     /gene="hda"
                     /locus_tag="JNC24_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HAY9159464.1"
                     /translation="MVNFSRFCEILVEVSLNTPAQLSLPLYLPDDETFASFWPGDNSS
                     LLAALQNVLRQEHSGYIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVP
                     EVLDGMEHLSLVCIDNIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNL
                     GLPDLASRLDWGQIYKLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRT
                     LFMTLDQLDRASITAQRKLTIPFVKEILKL"
     gene            complement(4249..5538)
                     /gene="uraA"
                     /locus_tag="JNC24_002761"
     CDS             complement(4249..5538)
                     /gene="uraA"
                     /locus_tag="JNC24_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HAY9159465.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPDSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            complement(5624..6250)
                     /gene="upp"
                     /locus_tag="JNC24_002762"
     CDS             complement(5624..6250)
                     /gene="upp"
                     /locus_tag="JNC24_002762"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HAY9159466.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            6575..7612
                     /gene="purM"
                     /locus_tag="JNC24_002763"
     CDS             6575..7612
                     /gene="purM"
                     /locus_tag="JNC24_002763"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HAY9159467.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            7612..8250
                     /gene="purN"
                     /locus_tag="JNC24_002764"
     CDS             7612..8250
                     /gene="purN"
                     /locus_tag="JNC24_002764"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HAY9159468.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTIRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            8421..10487
                     /gene="ppk1"
                     /locus_tag="JNC24_002765"
     CDS             8421..10487
                     /gene="ppk1"
                     /locus_tag="JNC24_002765"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HAY9159469.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNSHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRGDTIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYVASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            10492..12033
                     /gene="ppx"
                     /locus_tag="JNC24_002766"
     CDS             10492..12033
                     /gene="ppx"
                     /locus_tag="JNC24_002766"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HAY9159470.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEVLRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(12072..14315)
                     /gene="pdeF"
                     /locus_tag="JNC24_002767"
     CDS             complement(12072..14315)
                     /gene="pdeF"
                     /locus_tag="JNC24_002767"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HAY9159471.1"
                     /translation="MKLNATYIKMRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGIALGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFAL
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYMDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQYKQKLSHWLSPLLE
                     PGEDVYQLSGNDLALRLNTESHQERITALDSHLKQFRFFWDGMPMQPQIGVSYCYVRS
                     PVNHIYLLLGELNTVAELSIVTNAPENMQRRGAMYLQRELKDKVAMMNRLQQALEHNH
                     FFLMAQPITGMRGDVYHEILLRMKGENDELIVPDSFLPVAHEFGLSSSIDMWVIEHTL
                     QFMAENRAKMPAHRFAINLSPTSVCQARFPVEVSQLLAKYQIEAWQLIFEVTESNALT
                     NVKQAQITLQHLQELGCQIAIDDFGTGYASYARLKNVNADLLKIDGSFIRNIVSNSLD
                     YQIVASICHLARMKKMRVVAEYVENEEIREAVLSLGIDYMQGYLIGKPQPLIDTLNEI
                     EPIRESA"
     gene            complement(14497..14649)
                     /locus_tag="JNC24_002768"
     CDS             complement(14497..14649)
                     /locus_tag="JNC24_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9159472.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKTSVDTYFNVMEVNLIIYND
                     DVIMMN"
     gene            14667..14858
                     /locus_tag="JNC24_002769"
     CDS             14667..14858
                     /locus_tag="JNC24_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2633 family protein"
                     /protein_id="HAY9159473.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
BASE COUNT         3964 a         3818 c         3693 g         3512 t
ORIGIN      
        1 ctggcaactg gcgcacagta gcgcagacaa ttttccggca ctgacggtaa gccattttat
       61 taccgccaat ctgctcccgg tgatgctggg taatattatc ggcggtgcag tgctggtgag
      121 tatgtgttat cgggctattt atttacgtca ggaatcctga aaaatcagcc cggcgaaaca
      181 gttcgtcggg ctgaagatta ttcttgcgct atttgcccat cgggccaggc gtggatcacg
      241 gctttgatca gcgtcgccaa tggaatagcg aagaatacgc cccagaatcc ccacaaacca
      301 ccgaagatca ccaccgataa aataatcacc agcggatgca ggttaaccgc ttcggagaac
      361 aacaccggta ctaacagatt accgtccagc gcctgaataa tcagatacac tgcgaagcag
      421 ctccagaatt ccgtgcctgc accaaactgg aatagcgcca cgccaaccac cggaatggtc
      481 accacaaatg cgccaatgta cggaataagt accgagaagc cgaccagcac cgccagcagc
      541 agcgaatagt tcagcccaaa gagcaagaac cccagccaag tggcgatccc caccacgatc
      601 atctccagca ctttgccgcg gatatagttg gtgatttgtt gattcatctc cttccacacc
      661 tgtcctgcca gtccacggtt gcgcggcagc acccgacgaa cggcgttcag catctgctct
      721 ttgtctttca gcaggaagaa gaccatcaat ggtaccagca ccagatagac ggcaatggtc
      781 agcaaaccga ccagcgaggc gagggaaatt ttcaccaccg aatcgcccat ggtcagcatc
      841 cgactgcgca tattttcggc cattgcatca ataatgcccg cgtccattaa tgccggatag
      901 cggcgcggca acgtggcggc aaagtcagaa agcttattga gcatccccgg catatcgcgg
      961 atcaagtaga tgccctgttg ccaggcgata ggcagtacca cgaacgccat cagtagcaat
     1021 ataccgacga aaaccaccaa tacaatcgac gtcgcccagc ggcgggagca gccaatagat
     1081 tgcaggcgca cggttggcca ttccagcaaa taggccagca caatagccac cagcaacgga
     1141 gcaagcaggc cactaaagaa aaagataatg ccaaatccgg caactaaaat aaccagcaag
     1201 gcaatcgctt ccgggtcgct aaaacggcgg cgataccatt gcatcaacat ttcgagcata
     1261 aagaccttcc ctgaacctca agagcgggat tgcgatccgc aattgtatcg aaatgtcaca
     1321 aaaaagactt cgctttttat gacggattca ggaaactgaa aagtcatttg agtgggctaa
     1381 tcttcgccgt tacactcaaa ggcggcgcgg tgggaacaat atttcacagt atcggtcaaa
     1441 tgactatctt cagaaataca ggatagaggt tatgttcagg cagttgaaaa aaaacctggt
     1501 tgcaaccctc attgctgcta tgaccattgg tcaggtagcc ccggcgtttg ccgacagcgc
     1561 agacaccttg ccggatatgg gaacctccgc aggaagcacg ctttccattg gtcaggaaat
     1621 gcagatgggc gactattatg tccgccagct acgcggcagc gcgccgttaa ttaatgaccc
     1681 gctgttaacg caatatatta attcgctggg gatgcgtctg gtttcgcatg ccaattcggt
     1741 taagacaccg tttcattttt ttctgatcaa caacgacgaa attaacgcct ttgctttctt
     1801 tggcggcaac gtggtgctgc actctgccct gttccgttat tccgataacg aaagtcaact
     1861 ggcttcagtt atggcgcacg aaatctccca cgtcacccaa cgtcacctgg cgcgagcgat
     1921 ggaagatcag cagcgcagcg cgccgctgac ctgggtcggc gcgttaggtt ctattttact
     1981 ggcgatggcc agtccgcagg cggggatggc ggcgctgacc ggtacactgg cgggaacgcg
     2041 tcaggggatg atcagtttca cccagcaaaa tgaacaggaa gcggaccgca ttggtattca
     2101 ggtgctgcaa cgctcgggat tcgatccgca ggcgatgcca accttcctcg aaaaattact
     2161 cgatcaggcg cgttactcct cgcgcccgcc ggaaatttta ttgactcacc cgttgccgga
     2221 aagtcgtctg gcagatgccc gcaaccgtgc taatcagatg cgcccgatgg tggtgcagtc
     2281 gtcggaagat ttctatctgg cgaaagcgcg cacactgggg atgtataatt ccggacgtaa
     2341 ccagctcacc agtgatttgc tggatgaatg ggcgaaagga aacgttcgtc agcaacgagc
     2401 ggcgcaatat ggtcgtgctt tacaggcgat ggaagccaat aaatacgacg aggcgcgtaa
     2461 aacgctgcaa ccgttactgg cggcagaacc tggcaacgca tggtatctcg atctggctac
     2521 cgatatcgat cttgggcaaa acaaagccaa tgaggcaatc aatcgcctga aaaatgcccg
     2581 tgatttgcgc accaatccgg tgttgcagct caacctggca aacgcttatc tgcaaggcgg
     2641 tcaaccacaa gaagcggcca atattctgaa tcgctacacc tttaataata aagatgacag
     2701 caacggctgg gatttactgg cacaggcgga agccgcgcta aataaccgcg atcaggagct
     2761 ggctgcgcga gcagaaggtt atgcgctcgc cggacgactc gatcaggcca tttcgctgtt
     2821 gagtagcgcc agttcgcagg tgaaattagg cagccagcaa caagcgcgtt acgatgcgcg
     2881 catcgaccag ttgcgccagc tgcaggaacg ctttaagcct tataccaaga tgtaattcgc
     2941 attaaaggaa gaacaatgac caaacaggta aaaatctacc ataacccacg ctgttcaaag
     3001 agccgggaaa cgctgaacct gctgaaaaaa aacggcgtgg agccagaagt ggtactctac
     3061 cttgagacac ccgccgatgc ggcaacgctg cgcgatttgc tgaaaatgct ggggatgaat
     3121 agcgcccgtg aattgatgcg ccagaaagag gatctttata aagaactgaa cctggctgac
     3181 agttcgctta gcgaagaggc gctgattcag gcaatggtgg ataatccgaa gctgatggag
     3241 cgcccgattg tggtcgcgaa tggcaaagcg cggattggtc gtccaccgga gcaggtactg
     3301 gaaatcgtgg gttaagatgt gccccatcgc tagttgaagc acattgccgg atgcgacgct
     3361 tgccatgtct tatccggcct acgaatacta tggattaact tcgcgtagtt cggataaggc
     3421 gttcgcgccg catccgacaa taaacacctt atctacaact tcagaatttc tttcacaaac
     3481 ggaatggtca gcttacgttg cgcggtaatc gacgcacgat ccaactgatc caacgtcata
     3541 aatagcgtgc gcatttctct gtcgagccgc ttcagcaaga aacgccccac atcttccggc
     3601 agttcaaaac cacgcaaacg cgcgcgtaac tgtagcgcct gcaacttatc ttcatcagaa
     3661 agtggctgca atttgtagat ctgcccccag tcgagtcgcg acgcgagatc cggtaatccc
     3721 agattcaact gccgcggtgg acgatcgccg gtgatcaaca atcgtgtttt gcccgattcc
     3781 agaattcgat tgtagagatc gaaaatcgcc atctcccaca actcatcgcc tgcaatacac
     3841 tcaatgttgt cgatacagac cagcgacaaa tgctccatac cgtcgagcac ttccggaaca
     3901 aaccaggtgc gtttatccag cgggacatag cccaccgcat cgccacgctg cgacaattcc
     3961 gcgcaagccg cgtgcagcag atggctgcgc cccgcgcctt cgcgtgccca gagatagatg
     4021 taaccgctat gttcctgacg cagcacgttt tgcagcgcag ccagtaaaga ggagttatcc
     4081 cccggccaga aacttgcaaa ggtttcgtcg tcaggaagat aaagtggcaa agagagctgt
     4141 gccggtgtgt tcagagatac ctcaaccagg atttcacaaa atcgcgagaa gtttaccaca
     4201 gaatcccatg atgtttgaac cgggcagcaa cactgcccgg tcggtacatt atttgtctgt
     4261 tatatccgcg tcttctgcgt ccagcaccac ttcttcaggg cgcagcacac taatcagttt
     4321 gaagatcaaa cttaagccga taccgacgat ggtcgccagc gccatacctt tcagctccgc
     4381 cgcgccaatg tttaccttcg cgccactgac accgatgatc aaaatcacgg aagtcaggat
     4441 caggttctgt gctttgttgt aatccacttt tgattcgatc aaaacacgaa taccggaagc
     4501 accgatgaca ccataaagca gcagcgaaac gccgcccatc accggcaatg ggatcatctg
     4561 gatagcggca gccagtttac cgacgcagga aagcaggata gcgaaaatcg ccgccccacc
     4621 gataacccag gtactgtaaa cacgggtgat cgccatcacg ccaatgtttt ctccgtaagt
     4681 ggtatttggc gtagagccaa agaagccgga gatcacagtc gacaggccgt tagcaaacat
     4741 cgaacggtgc agacctggat cgcgcagcag atcttttttg acgatattag ccgttactac
     4801 caggtgccct acgtgttcgg caataaccac taacgccgct ggcagaatag tcagaatggc
     4861 aaaccactcg aagcgcggcg tatagagggt tggcagcgca aaccagtgag cattaataat
     4921 cggcgtggta tcgacaattc ccattgcgaa agagagcgcg taccccacca gcacgccaat
     4981 taaaatcggg ataattgcca ggaaaccacg gaacagcacg gaacctaaaa ccgtgaccgc
     5041 cagggtggta atagagatga tgatggtttt ggagtctggc gtttgccctt cagccgggag
     5101 taaacccgcc ataccggcag ctacgcccgc cagctccaga ccgatgacgg caacgattgc
     5161 gcccattgcc gcaggtggaa acagcacgtc cagccagccg gtccccgctt tcttcacgat
     5221 aaaagaaacc aggcagaaca gcacgccgca cataataaag ccgcccagcg cgacttcata
     5281 ccctaacggc aacagtaaca ataccggtga aataaaggca aagctggaac caagataagc
     5341 cggaattttc cctttacaga tgaagagata cagcagcgtt ccaataccgt taaataacag
     5401 tacagtcgcc gggttaatat gaaataagac gggcaccagg acggttgcac caaacatggc
     5461 gaacaaatgt tgcaaactaa gcgggattgt ctgtaaaagt ggcggtcttt cactcacccc
     5521 gatagcacgg cgcgtcatag tattatcctc tgtattatgt gttataggcg ctttactcaa
     5581 aaaaaagccg actcttaaag tcggctttaa ttatttttat tctttatttc gtaccaaaga
     5641 ttttgtcacc ggcatcgccg aggcccggaa taatgtatcc gtgctcgttc agtccctggt
     5701 caatcgatgc ggtatacagt tcgacgtccg ggtgcgcttt ttccagcgca gcgatacctt
     5761 ctggcgcagc taccagcacc agaactttga tgctgctgca gcccgctttt ttcagcaggt
     5821 cgatggtcgc gataacggaa ccaccggttg ccagcattgg gtcaacgatc agcgccatac
     5881 gctcatcgat gttagaaacc agtttctgga agtacggtac cggctccagc gtttcttcat
     5941 tacggtacat accgacaacg ctgatgcgcg cgctcggaac attttccagc acaccgtcca
     6001 tcatccccag accggcacgc agaattggca caacggtaat tttcttacct ttgatctggt
     6061 cgatttctac cgggccgttc cagccttcga tagttacttt ttccgtttcg aggtcggcgg
     6121 tcgcttcgta agtcagcagg ctacccactt cggaagcgag ttcgcgaaag cgcttggtgc
     6181 tgatatcttg ctcacgcatc agtcccagct tgtgtttgac gagtgggtgt ttgacttcca
     6241 cgatcttcat actctttctc ctttgagggg cagccacaaa aaaaatcgac ggattatacc
     6301 tcctttcttc aaggcggcaa tattcttttc gttgacttta gtcaaaatga taacggtttg
     6361 agataaagtt attttatatt caaatggtta tgaaagaaga ttattccatc cgaaaactaa
     6421 cctttgccct ggcacaagtc ttctttcgcc gcgcgtctgg ggaaaagacg tgcaaaaagg
     6481 ttgtgcaaag cagtctcgaa aacgtttgct ttccctgtta gaattgcgcc gaattttatt
     6541 tttctaccgc aagtaacgcg tggggaccca agcagtgacc gataaaacct ctcttagcta
     6601 caaagatgcc ggtgttgata ttgacgcggg taatgctctg gttggaagaa tcaaaggcgt
     6661 agtgaagaaa acgcgtcgtc cggaagtgat gggcggtctg ggcggcttcg gtgcgctgtg
     6721 tgcattgccg caaaaatatc gtgaacccgt gctggtttcc ggcactgacg gcgtaggtac
     6781 caagctgcgt ctggcaatgg acttaaaacg tcacgacacc attggtattg atctggtcgc
     6841 catgtgcgtt aatgacctgg tggtgcaagg tgcagaaccg ctgtttttcc tcgactatta
     6901 cgcaaccgga aaactggatg ttgataccgc ttcagcggtg atcagcggta tcgcggaagg
     6961 atgtctgcaa tcgggctgtt cactggtggg tggcgaaacg gcagaaatgc cggggatgta
     7021 tcacggtgag gattacgatg tcgcgggttt ctgcgttggc gtggtagaaa aatcagaaat
     7081 catcgacggc tctaaagtca gcgacggcga tgtgctgatt gcactcggtt ccagcggtcc
     7141 gcactcgaac ggctattcgc tggtgcgcaa aattcttgaa gtcagcggtt gtgatccgca
     7201 aaccaccgaa cttgatggta agccattagc cgatcatctg ctggcaccga cccgcattta
     7261 cgtgaagtca gtgctggagt tgattgaaaa ggtcgatgtg catgccattg cgcacctgac
     7321 cggcggcggc ttctgggaaa acattccgcg cgtattgcca gataatactc aggcagtgat
     7381 tgatgaatct tcctggcagt ggccggaagt gttcaactgg ctgcaaacgg ctggtaacgt
     7441 tgagcgccat gaaatgtatc gcaccttcaa ctgcggcgtc gggatgatta tcgccctgcc
     7501 tgctccggaa gtggacaaag ccctcgccct gctaaatgcc aacggtgaaa acgcgtggaa
     7561 aatcggtatc atcaaagcct ctgattccga acaacgcgtg gttatcgaat aatgaatatt
     7621 gtggtgctta tttccggcaa cggaagtaat ttacaggcaa ttattgacgc ctgtaaaacc
     7681 aacaaaatta aaggcaccat acgggcagtt ttcagcaata aggccgacgc gttcggcctt
     7741 gaacgcgccc gccaggcggg tattgcaacg catacgctca tcgccagcgc gtttgacagt
     7801 cgtgaagcct atgaccggga gttgattcat gaaatcgaca tgtacgcacc cgatgtggtc
     7861 gtgctggctg gttttatgcg cattctcagc ccggcgtttg tctcccacta tgccgggcgt
     7921 ttgctgaaca ttcacccttc tctgctgccg aaatatcccg gattacacac ccatcgtcag
     7981 gcgctggaaa atggtgatga agagcacggt acatcggtgc atttcgtcac cgatgaactg
     8041 gacggtggcc cggttatttt acaggcgaaa gtcccggtat ttgctggtga tacggaagat
     8101 gacatcaccg cccgcgtgca aacccaggaa cacgctattt atccactggt gattagctgg
     8161 tttgccgatg gtcgtctgaa aatgcacgaa aacgccgcgt ggctggatgg tcaacgtctg
     8221 ccgccgcagg gctacgctgc cgacgagtaa tgccccgtag ttaaagcgcc agctctgccg
     8281 ctggcgtttt tcaattcacc tgtaaatcgc aagctccagc agtttttttc ccccttttct
     8341 ggcatagttg gacatctgcc aatattgctc gccataatat ccaggcagtg tcccgtgaat
     8401 aaaacggagt aaaagtggta atgggtcagg aaaagctata catcgaaaaa gagctcagtt
     8461 ggttatcgtt caatgaacgc gtgcttcagg aagcggcgga caaatctaac ccgctgattg
     8521 aaaggatgcg tttcctgggg atctattcca ataaccttga tgagttctat aaagtccgct
     8581 tcgctgaact gaagcgacgc atcattatta gcgaagaaca aggctccaac tctcattccc
     8641 gccatttact gggcaaaatt cagtcccggg tgctgaaagc cgatcaggaa ttcgacggcc
     8701 tctacaacga gctgctgctg gagatggcgc gtaaccagat cttcctgatt aatgaacgcc
     8761 agctctccgt caatcaacaa aactggctgc gtcattattt taagcagtat ctgcgtcagc
     8821 acattacgcc gattttaatc aatcctgaca ctgacttagt gcagttcctg aaagatgatt
     8881 acacctatct ggcggtggaa attatccgtg gcgataccat ccgttacgcg ctgctggaga
     8941 tcccatcaga taaagtgccg cgctttgtga atttaccgcc agaagcaccg cgtcgacgca
     9001 agccgatgat tcttctggat aacattctgc gttactgcct tgatgatatt ttcaaaggct
     9061 tctttgatta tgacgcgctg aatgcctatt caatgaagat gacccgcgat gccgaatacg
     9121 atttagtgca tgagatggaa gccagcctga tggagttgat gtcttccagt ctcaagcagc
     9181 gtttaactgc tgagccggtg cgttttgttt atcagcgcga tatgcccaat gcgctggttg
     9241 aagtgttacg cgaaaaactg actatttccc gctacgactc catcgtcccc ggcggtcgtt
     9301 atcataattt taaagacttt attaatttcc ccaatgtcgg caaagccaat ctggtgaaca
     9361 aaccactgcc gcgtttacgc catatttggt ttgataaagc ccagttccgc aatggttttg
     9421 atgccattcg cgaacgcgat gtgttgctct attatcctta tcacaccttt gagcatgtgc
     9481 tggaactgct gcgtcaggct tcgttcgacc cgagcgtact ggcgattaaa attaacattt
     9541 accgtgtggc aaaagattca cgtatcatcg actcgatgat ccacgctgcg cacaacggca
     9601 agaaagtgac cgtggtggtt gagttacagg cgcgtttcga cgaagaagcc aacattcact
     9661 gggcgaagcg cctgaccgaa gcaggcgtgc acgttatctt ctctgcgccg gggctgaaaa
     9721 ttcacgccaa actgttcctg atttcacgta aagaaaacgg tgaagtggtc cgttacgcac
     9781 acatcgggac cgggaacttt aacgaaaaaa ccgcgcgtct ttatactgac tattcgttgc
     9841 tgaccgcaga tgcgcgtatc accaacgaag tacggcgggt atttaacttt attgaaaacc
     9901 cataccgccc ggtgacattt gattatttaa tggtgtcacc gcaaaactct cgccgtctgt
     9961 tatatgaaat ggtagaccgc gaaatcgcca acgcgcagca agggctgccc agtggtatca
    10021 ccctgaagct aaataacctt gtcgataaag gcctggttga tcgtctgtat gtggcctcca
    10081 gctccggcgt accggttaat ctgctggttc gcggaatgtg ttcgctgatc cccaatctgg
    10141 aaggcattag cgacaacatt cgtgccatca gtattgttga ccgttacctt gaacatgacc
    10201 gggtttatat ttttgaaaat ggcggcgata aaaaggtcta cctttcttcc gccgactgga
    10261 tgacgcgcaa tattgattat cgtattgaag tggcgacacc gctgctcgat ccgcgcctga
    10321 agcagcgggt gctggacatc atcgacatat tgttcagcga tacggtcaaa gctcgttata
    10381 tcgataaaga actcagtaat cgctacgttc cccgcggcaa tcgccgcaaa gtacgggcgc
    10441 agttggcgat ttatgactac atcaaatcac tcgaacaacc tgaataaccc tatgccaata
    10501 cacgataaat cccctcgtcc gcaggagttt gctgcggtcg atcttggttc aaacagtttt
    10561 cacatggtca tagcccgtgt ggtagatggt gccatgcaga ttattggccg cctgaaacag
    10621 cgggtgcatc tggcggacgg cctggggcca gataatatgt tgagtgaaga ggcaatgacg
    10681 cgcggtttga actgtctgtc gctgtttgcc gaacggctac aagggttttc tcctgccagc
    10741 gtctgtatag tcggcaccca tacgctgcgt caggcgctga acgccactga ctttctgaaa
    10801 cgcgcggaaa aggtcattcc ctacccgatt gaaattattt ccggtaatga agaagcccgt
    10861 ctgattttta tgggcgtgga acatacccaa ccggagaaag gtcgcaaact ggttattgat
    10921 attggcggcg gatctacgga actggtgatt ggtgaaaatt tcgaaccgat tctcgttgaa
    10981 agccgccgga tgggttgtgt cagctttgcc cagctttact tccctggcgg ggtcatcaat
    11041 aaagagaatt ttcagcgcgc tcgtatggcg gcagcacaaa aactggaaac tttaacctgg
    11101 caattccgta ttcagggctg gaacgtggca atgggcgctt ccggtaccat aaaagccgcc
    11161 catgaagtgt taatggaaat gggcgagaaa gacgggataa ttaccccgga acgtctggaa
    11221 aaactggtaa aagaagtttt acgtcaccgt aatttcgcat cgctgagttt accgggtctt
    11281 tccgaagagc ggaaaacagt cttcgttcct ggactggcga ttttatgcgg tgtgtttgat
    11341 gctttagcca tccgtgaact gcgcctttct gacggggcgt tacgcgaagg cgtactgtac
    11401 gaaatggaag gacgtttccg tcatcaggat gtgcgtagtc gcaccgccag cagcctcgcc
    11461 aaccagtatc acatcgacag cgaacaggcc cgacgggtgc tggataccac tatgcaaatg
    11521 tacgaacagt ggcgggaaca gcaaccgaag ctggcgcatc cgcaactgga ggcgctactg
    11581 cgatgggccg ccatgctgca tgaggtcggg ttgaatatca accacagcgg tttgcatcgc
    11641 cactccgctt atattctgca aaacagtgac ttgccgggtt ttaatcagga acagcagctg
    11701 atgatggcga cactggtgcg ctatcaccgt aaagcgatta aactcgacga tctgccgcgc
    11761 tttaccttgt ttaagaagaa acagttcctg ccactgattc agctattgcg ccttggcgta
    11821 ttactcaaca atcaacgtca ggcaaccacc acaccgccaa cattgacact gattactgat
    11881 gacagtcact ggacactgcg tttcccgcat gactggttta gtcagaatgc gttggtactg
    11941 cttgatctgg aaaaggagca agaatactgg gaaggcgtgg ctggctggcg gttgaaaatt
    12001 gaagaagaaa gtacacctga aatcgccgct taatgctttg cgggccgacg agaaatgtcg
    12061 gcccgcatta ttcaggcact ttcgcgaatg ggttcgattt cattcaacgt atcaattaac
    12121 ggctgcggct taccaataag ataaccctgc atataatcga tccccaaaga gagcaccgcc
    12181 tcgcggatct cttcgttttc aacgtactct gccactaccc gcattttctt cattcgcgcc
    12241 agatggcaaa tcgacgccac aatctgataa tccagactat ttgacacaat attgcggata
    12301 aagctgccgt caattttaag cagatcggca ttcacatttt taagccgtgc atagctggcg
    12361 tagccggtgc cgaaatcatc aatcgcaatc tggcagccta attcctgaag atgctgcaag
    12421 gtgatttgcg cctgcttaac attggtcaga gcattacttt cggtgacttc aaaaataagt
    12481 tgccacgctt caatctgata tttagccagc aactgactga cttcaacagg aaaacgagcc
    12541 tgacataccg aggttggaga cagattaata gcaaaacggt gagcgggcat cttcgctctg
    12601 ttttcagcca taaattgcag cgtatgctca atgacccaca tgtcgatact cgacgataaa
    12661 ccaaattcgt gcgcgaccgg caagaagcta tcggggacga tcagttcatc attctcacct
    12721 ttcatgcgca gaagaatttc atggtaaaca tcaccacgca taccggtaat cggctgggcc
    12781 atcaggaaaa aatggttgtg ttccagcgcc tgctgtagtc gattcatcat cgcgacttta
    12841 tctttcaatt cgcgttgcaa atacattgcc ccgcgacgct gcatattttc cggggcgttg
    12901 gtcacgatgg aaagttcggc gaccgtattt agctctccca gcagcaggta gatatgattc
    12961 actggcgagc gcacatagca gtaactgacg ccaatctgcg gttgcatcgg catcccatcc
    13021 caaaagaaac gaaattgctt gagatgacta tccagtgcgg taatgcgctc ctggtgcgat
    13081 tctgtattca gtcgcagcgc gagatcgtta cccgaaagct gataaacatc ttcacccggt
    13141 tccagcaagg gtgacagcca gtgagaaagt ttttgcttgt attgaatccg cagcatgatg
    13201 ccatagttct taaccagcat ttccatgcca gggatgcgta aataacaaag cgcagaccag
    13261 ggagcatcac gcaacgcgcg attcagggcg cgaacatttg gcagatgaac caccgggtcc
    13321 atatacgcaa gccgctgaat gcgtctgact accgctctct gacgggttgc cagtacagcc
    13381 atataattga caataaaaga gaataccaga taactggagg aggttatggt cagctgcgtg
    13441 gtatagccag gataaatggg aatgtaattt tgatagctgt ggatgctgat catcaacacg
    13501 accgcccaga gcagcgaaat cagcttataa ccatagcgca tcgctcccca catcatcagg
    13561 ggcagcaata atgacaaggt ataattggtg ctaaaaattg tgcttttttc atttaacggc
    13621 atgcataaca gcaacaataa agcacctaat gccagtagcc agagcgcgaa ctcttttttg
    13681 gtgactttgg catcaacctg ctgttttaat tgcgaataat agctacgtaa ataaaatgga
    13741 tttcgcacta cccgaatgat gaagtagcac agcgggacac cgatcagatt acccaccagt
    13801 aaggcctgat aattgattaa agtcccgagg ttaaaaggca tgacaccgac cagattttct
    13861 ctgctcgcca gtaatcctac aaatgcagca aactggaaaa gtatcagaaa cagcgttgca
    13921 ggaaacacaa tctgccagaa tatacgctgg gaaattaaac gggtatcacc atgtgaaacg
    13981 ttgttacgcc ggggagtaaa gaccctgtaa ccgccccaac agagaatgat aatgataata
    14041 aaattagccg ttaatgatag cgtttcgtaa aaacccagct ctgcatattt gcggacaaaa
    14101 atccctaagg cgatccctgg caatgccgcc cagctgaaaa acatcatcat gctgatcatc
    14161 agtgccagag gcagataaaa gagaaaaacc tcaccggaag aaatatgcgc gaaagtatta
    14221 atgtgggcga aaatgggcaa gattaaagaa ggcaggaaca gcggaagccc ccaccattta
    14281 tcacgcattt ttatataagt tgcattcagt ttcatagatg ctcagcagaa tcccccacat
    14341 cctgaaggag gtgtattcag acaggcatcc cacctgactt cgaatgatga ttattcatca
    14401 ctatagagag cattgattct aagtgtcata tgaaagttcc aattgatata tatcaaacaa
    14461 aataaccctg attaatgaat tattacgttt atcatgttaa ttcatcatta ttacatcatc
    14521 attgtaaata attaaattaa cttccataac attaaaatat gtatccactg acgttttttt
    14581 acataacgaa gaattgacca ttttgtcctg ttgtgcctta atgtaagtac cgtccacagc
    14641 gtgggacata cttcaaggaa ccttttgtga gtcaggcaac cagtatgcga aaacgacacc
    14701 gatttaacag tcgcatgacc cgtatcgtac tgctcatcag ctttatcttc ttctttggcc
    14761 gttttatcta ctcgtccgtc ggtgcctggc agcaccatca gagcaaaaaa gaagctcagc
    14821 aatccacact ctccgtcgaa tcaccggtac aacgttagcg gttaccttct tcactttcac
    14881 agaacataac ggcacttcgc tgtcggatgc ttttgctctt tgggattatc aaagcggcag
    14941 atattctttc atcttaaatt ttacgtcttt gtcctgactg atgttta
//
LOCUS       DADMKF010000099        14965 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.101, whole genome shotgun sequence.
ACCESSION   DADMKF010000099 DADMKF010000000
VERSION     DADMKF010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14965
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.101"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            313..1611
                     /gene="kgtP"
                     /locus_tag="JNC24_002770"
     CDS             313..1611
                     /gene="kgtP"
                     /locus_tag="JNC24_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HAY9159474.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HVNVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(1608..1952)
                     /locus_tag="JNC24_002771"
     CDS             complement(1608..1952)
                     /locus_tag="JNC24_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708437.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HAY9159475.1"
                     /translation="MFFGGHVLRILFVCSLLLLSGCSHMANDSWSGQDKAQHFIASAM
                     LSAAGNEYSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGA
                     STGYTVWQLTRH"
     gene            complement(1977..3332)
                     /gene="pssA"
                     /locus_tag="JNC24_002772"
     CDS             complement(1977..3332)
                     /gene="pssA"
                     /locus_tag="JNC24_002772"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HAY9159476.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENSGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLKLAPQREKELELIREHTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3446..6106)
                     /gene="pat"
                     /locus_tag="JNC24_002773"
     CDS             complement(3446..6106)
                     /gene="pat"
                     /locus_tag="JNC24_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HAY9159477.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGSFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPEHV
                     AVSNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPTLPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDISPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRDHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLAQREES"
     gene            complement(6138..6836)
                     /locus_tag="JNC24_002774"
     CDS             complement(6138..6836)
                     /locus_tag="JNC24_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DTW domain-containing protein"
                     /protein_id="HAY9159478.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVHRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAAGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            complement(6905..7324)
                     /gene="trxC"
                     /locus_tag="JNC24_002775"
     CDS             complement(6905..7324)
                     /gene="trxC"
                     /locus_tag="JNC24_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HAY9159479.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7531..8568
                     /locus_tag="JNC24_002776"
     CDS             7531..8568
                     /locus_tag="JNC24_002776"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HAY9159480.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVSQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(8616..9305)
                     /gene="ung"
                     /locus_tag="JNC24_002777"
     CDS             complement(8616..9305)
                     /gene="ung"
                     /locus_tag="JNC24_002777"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAY9159481.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAESE"
     gene            9610..9993
                     /gene="grcA"
                     /locus_tag="JNC24_002778"
     CDS             9610..9993
                     /gene="grcA"
                     /locus_tag="JNC24_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HAY9159482.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(10049..10636)
                     /gene="eamB"
                     /locus_tag="JNC24_002779"
     CDS             complement(10049..10636)
                     /gene="eamB"
                     /locus_tag="JNC24_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HAY9159483.1"
                     /translation="MTPTLLSAFWTYTLITAMTPGPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            10739..11620
                     /locus_tag="JNC24_002780"
     CDS             10739..11620
                     /locus_tag="JNC24_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY9159484.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQSLNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIVDVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     ISIESIKRCVAANIGVSYLPRFAVAKELECGELIELPFGEQSQTITAMCAHHAGKAVS
                     PAMHTFIQCVEESFVAG"
     gene            complement(11829..13163)
                     /gene="srmB"
                     /locus_tag="JNC24_002781"
     CDS             complement(11829..13163)
                     /gene="srmB"
                     /locus_tag="JNC24_002781"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HAY9159485.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            13295..14032
                     /gene="trmN"
                     /locus_tag="JNC24_002782"
     CDS             13295..14032
                     /gene="trmN"
                     /locus_tag="JNC24_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HAY9159486.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWLTQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQVGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            complement(14017..>14965)
                     /locus_tag="JNC24_002783"
     CDS             complement(14017..>14965)
                     /locus_tag="JNC24_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HAY9159487.1"
                     /translation="PLLPVHLSRVANLEFNQFPPTALYHPQARNFLLTEALRGEGAYL
                     KRPDGTRFMPDFDERGELAPRDIVARAIDHEMKRLGADCMFLDISHKPADFIRQHFPM
                     IYEKLLGLGIDLTQEPVPIVPAAHYTCGGVMVDDHGRTDVEGLYAIGEVSYTGLHGAN
                     RMASNSLLECLVYGWSAAEDITRRMPDAHGVSTLPPWDESRVENPDERVVIQHNWHEL
                     RLFMWDYVGIVRTTKRLERALRRINMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIV
                     RCAMMRKESRGLHFTLDYPELLTHSGPSILPPGNHYINR"
BASE COUNT         3612 a         3784 c         3922 g         3647 t
ORIGIN      
        1 aaaggccatc ctgacggatg gcctttttgc attggcgcag aaaaaaatgc ctgatgcgac
       61 actgcgcgtc ttatactccc acatatgcca gattcagcaa cggatacggt ttccccaact
      121 tgcccacttc catacgtgtc ctccttacca gaaatttatc cttaagctcc tcaataacca
      181 ttttcctgct aactaaattc atggttaagg ttgcacaatg atatgcaaca aatgtataac
      241 attcccttta ccaaaaaaaa taaacaaaag cgaccgacaa aagcatcgga ttacggcagg
      301 agacataatg gcatggctga aagtactgta acggcagaca gcaaactgac aagtagtgat
      361 actcgtcgcc gcatttgggc aattgtgggg gcctcttcag gtaatctggt cgagtggttc
      421 gatttctatg tctactcgtt ctgttcactc tacttcgccc acatcttctt cccttccggg
      481 aacacgacga ctcaactact acaaacagca ggtgtttttg ctgcgggatt cctgatgcgc
      541 ccaataggcg gttggctatt tggccgcata gccgataaac atggtcgcaa aaaatcgatg
      601 ctgctatcgg tgtgtatgat gtgtttcggc tcactggtaa tcgcctgttt gcctggttat
      661 gaaacaatcg gtacgtgggc tccggcatta ttgcttctcg cgcgtttatt tcagggatta
      721 tccgttggcg gagaatatgg caccagcgcc acctatatga gtgaagttgc cgttgaaggg
      781 cgcaaaggtt tttacgcatc atttcagtat gtgacgttga tcggcggaca actgctagcc
      841 ctactggttg tcgtggtttt acaacacacc atggaagacg ctgcactcag agagtgggga
      901 tggcgtattc ctttcgcgtt aggagctgtg ttagctgttg tggcgttgtg gttacgtcgt
      961 cagttagatg aaacttcgca acaagaaacg cgcgctttaa aagaagctgg atctctgaaa
     1021 ggattatggc gcaatcgccg tgcattcatc atggttctcg gttttaccgc tgcgggctcc
     1081 ctttgtttct ataccttcac tacttatatg cagaagtatc tggtaaatac tgcgggaatg
     1141 catgtcaacg tggcgagtgg cattatgact gccgcattgt ttgtattcat gcttattcaa
     1201 ccactcattg gcgcgctgtc ggataagatt ggtcgccgta cctcaatgtt atgtttcggt
     1261 tcgctggcag ccatttttac cgttcctatt ctctcagcat tgcaaaacgt ttcctcgcct
     1321 tatgccgctt ttggtctggt gatgtgtgcc ctgctgatag tgagttttta tacatcaatc
     1381 agtggaatac tgaaggctga gatgttcccg gcacaggttc gcgcattagg cgttggcctg
     1441 tcatatgcgg tcgctaatgc tatatttggt ggttcggcgg agtacgtagc gttgtcgctg
     1501 aaatcaatag gaatggaaac agccttcttc tggtatgtga ccttgatggc cgtggtggcg
     1561 tttctggttt ctttgatgct acatcgcaaa gggaagggga tgcgtcttta gtgacgggtc
     1621 agttgccaga cggtatagcc ggtgcttgca ccggcgacat cccaggccaa atccttccag
     1681 ctccagccgc tcccttcggg gcggctatcc caaagctctt ttgacgcccc caaactgaca
     1741 gagaacatca atccaaacat ggcactgcga tcccggctca tcccctgatg ctgtgaatat
     1801 tcatttccgg cggcggaaag catcgccgag gcgataaagt gttgagcttt atcctgcccg
     1861 ctccagctat cgttagccat atggctgcat ccagaaagta acaatagcga acagacaaaa
     1921 agaatacgca aaacgtggcc tccaaaaaac aaaccccgta caggacgggg ttgtgattac
     1981 aggatgcggc taattaatcg gtcgatgcgg atacggcgca aacggcggat gagtttacga
     2041 accttcaccg gataatcggc aatactttgc agatcgcgat agtgcttaac gatggtggta
     2101 tgctcgcgga tcagctccag ttctttctct cgctgcggcg caagcttcag ttgtggatcg
     2161 tggatcaaaa tggcgttttc cagatccaga cgccaggcgc gcgggttcag gttattaccg
     2221 gtgatcaaca tccacttatc atcaacccac atccctttca ggtgataggt gttgtcgtca
     2281 tctttccata accgaaccac tagctggtca gtattgacgt aatactgcaa acggctcagg
     2341 aaacgacgca gattgatctc atagagataa ggcaatgcgc caattatctt gaaaggttca
     2401 tcttccggaa tgtagaagtc gttcgcggtt ttatcaccaa caataatttc gacctttttc
     2461 ccttcgcgca gcaactggat aatattgcgc acaaggattg ctggcaggtt gaagtatggc
     2521 gtacagatgg ttagtttctg ctcggcacaa ggcataagat ggaaaatggt cttgttcaac
     2581 agactcgatt tccccagccc cactagcggc gttacggaaa gctgatcgtt gtcggcatcg
     2641 ccctggaaat gataagcggc atcacgcagc tcctggcgga acagacgaat atcgttcttg
     2701 atttccgggc tttttggccg attaacatca tccagacgat taacgccgcg gccattcata
     2761 atattctgtg taacccattc aaacataatg tctgacatct tacggttacg gataagatga
     2821 taacggtcgt agcgatattt atcgtgctga tgcaggtaaa catcgttcag gctggcaccg
     2881 ctataaagta cgctatcgtc gatgataaag cctttaaagt gcagaacacc aagggcttca
     2941 cgggtattga ttggaacgcc ataaaccgga acatctacgc ccgaattttc ctgcgccatg
     3001 cggcagtacc agtcagcgtt agtgttagat gccgcagcgc caatgcgtcc acgttgtgca
     3061 cgatgccagt cgaccagcac ccgcacatcc agttccggac gctgcctttt agcttcatac
     3121 aacgcgttca gaatgccttt gccaccgtca tcctgttcga gatacagggc gacaatgcaa
     3181 atgcgctgct tcgcgctggc tattttttcc agcagcgtct cccggaagtc ggcgggagcg
     3241 taaaagaaat cgacatcatc aactgattga gaaatcttgg gtagttgggc aaggtgttgt
     3301 tgatgtttat tacgcttaaa ttttgacaac atcacagtgc atttcttctc tgttcattga
     3361 agggtcctct gtgcaatgca gacgacataa gcgggcaata ataacaccag tcccgattaa
     3421 gtggtcaaca tttccagtac cttactcatg attcctcgcg ttgggcaaga tttagcgtaa
     3481 gcccaacgat cccctcttcg agctggatat caacgttaaa cccgagcttg cgggctagcg
     3541 ccaccatgcc acgattgttt ggcatcgtaa taccattcag acgttgtagt ccgtgatctc
     3601 gcgtataggt aatcaacttt tccattaagc gtcgacctaa gcctaaccct ttgagatccg
     3661 agcgaaccag cacagcaaat tcggcatcga tgttatcagg atcggaaatc gcacgcgtga
     3721 cgccgaggat ctcttccgtt tgatcaatac gtcgtaccgc tacaaacgcc atttcccgat
     3781 cgtagtcgat ctgtgtcatg ttggctaaat cttcatgggt aaattcgttg atctcgctaa
     3841 agtagcggta ataaagatct tctttggtga ctcgcgaaat gaattgctga agttgtggct
     3901 catcttctgg caaaatcggg cggaacaagc agcgttcacc gtttttcaat tctacccatt
     3961 cttccagctg atgcggataa gggcgcactg ccagccgact ctcgttatcg ccttcaaacg
     4021 gcgagatatc cagcgtgaca tccagcgcgg taaattcact gccagaagcc agcaaaggat
     4081 gaatatccag acgctgaatt tccgggcaat cgacaatcaa gttggaaacc tgcaccagaa
     4141 gctggctcaa gcctgcaaca tccaatgggc gtagcgcact gcgcgcacga atctttttac
     4201 ttttgatccc ctgaataacc agatagcggg ccaggttcat gttcagcggc ggcagtgcga
     4261 cgacggcttg atcttcagga cgccactcca caccgccttc acccagcatg atcaacggcc
     4321 cgaaaaccgg atcgtgctca accacaaccc gcaactcctg agcgccagca cggttagcca
     4381 tactttgcac caacaggccg tggacccgcg cctgtggcca ggccattttt acgcgatcga
     4441 aaatagcgtt cgccgcttgc tggacttcat tggctgtacg caggtaaagc atgacgccct
     4501 gaacttccga tttatgtgga atatccggcg aacgcaattt cagcgccacc ggataaccaa
     4561 tctgttcggc aatatgcacc gcttcggtgc tatcgctggc aatccaggta gggagcgtgt
     4621 tcatgccata cgcttgcagg atgggctgaa cttcatgggt atcgagcgac gtagcccctt
     4681 cggcaatcgc ctgttgcaac agaagatgcg cttctgcggt attggaagtc agattgctgg
     4741 gcaacgtcgg cgtttcgcgt agttgcttct gattacgccg gtactccacc atatgcataa
     4801 aagcagtgat ggttccttcc ggggtacggt aggtcggcag cccggcttcg ctgaataaac
     4861 gtcgtgcctc ttgcgaggag tgctcgccgc accagttcgt cagcaaagag acatatttgc
     4921 tgcggggatg atgctttacc gcttcaatta atacttgcgc gctttctgtt gcgggagcag
     4981 cggcgctggg cgaatgaata accatcagcg catcaaaatc ctggctgtgg agcagaatgt
     5041 ccagcgtttt gacataatgc tcgctgctgg cgtcatcgcg cagatcgaga gggttagaga
     5101 ctgccacatg ttctggcaat gcatcgcgca atttctggca ggtttcttcg cttagcgttg
     5161 ccagcttgcc attgcgtgac cataaggcat ccagcgccag cgcggcaggc gcagcaccgt
     5221 tgctgataat catcaaccgg tcgccacgca gcgggcgcat atggctaagg gtttccaccg
     5281 ccgaaaacag ttcgtgggta tcctgtaccc gcaacaaacc ggcacgctga atagccgcat
     5341 cccatgccgg gtccattcct gccgtcgtgt tgagcagtcg ctgtgccgcc gggctacgtc
     5401 cgcttttaat caccagaatc ggtttattac gcgaggcact acgggctgcc gaaacaaagc
     5461 gtcgcgcgtc gcttaactgt tcgagataga gcaggatggc gctggtttta ctgtcgcgcg
     5521 ccagatagtc gagcaattca tcaacgtcga tatccaggct gtcgccgagc gcaataaagt
     5581 aggaaaagcc catctcacgc tgttgcgccc agtcgaggat ggtgttggag acggctgccg
     5641 attgtgaaat aaacgccagc ttgccgcgtt taatcggcac aggcgaaaag ctggcattca
     5701 gaccttgcca gggagccagt aaacccagac tgtttggtcc aagcaggcgc atgttatggc
     5761 gcagggcgca ggcgcggaga tcttcgtgtt gcgatgccgg ggcggaaaga ataatgcagg
     5821 ttttacagcc tttctcgccg agctcttcca gaagagcaag attacggctg gcattggtac
     5881 ataaaaccgc aaggtcgggt gtaaagggca agctggcaat atccggccag gccaacacac
     5941 ccaacaccgc tttccaggct ggcgtcaccg ggagtaccgg tccgttaaag cttcccgcca
     6001 gcaggttacg catcatcagg taacctgcgc gattgggttt catcgacgcg ccaattaccg
     6061 ctatcgattt tggtcgcagt agtgcttcca gtcctcgctg actcataccg gtctcccgca
     6121 gaagtgaccg aatgatttta aacgctttct aactgttctg ctgtgatgct acccagatgt
     6181 tgcgtttttc ctgccagata gcgtgtttta aagcgggtaa aatgctcgcc taaccctgct
     6241 gccgccccgg tatcgccggc catatctaac agtgcgatgg ctacctcggc agtacaatat
     6301 tggcctgcag cctgggcttc acgcaggcga taggcagaaa gccgggaaag atcgacggaa
     6361 atgacgggaa gattatccag atacggactt ttacgaaaca tcttgcgagc ttccggccag
     6421 gtaccatcga gcatgataaa cagcggtggc ttaccggcag gtggtgtgaa gatcacttcc
     6481 cgttgctcat cagcatacga ggcgggaaag accaccattg gctgataatc cgggttttgt
     6541 accagatcca gcaaatcctg cgagggttcg gtacgcgacc attgaaacgc aacggtatca
     6601 ggcaaaatat cagcaatcag acgcccggta ttactgggct tcattggctc ggtgtcgaac
     6661 atcagcaaac agaagcgact ttttgcttgt gctggggtaa ttgtcgaaca gagacataat
     6721 ttctctggca aaagacagcg ttggcagcga tgaacgcgat taccgcgggc aagaaaagga
     6781 cgtgttgcgc gcgcaatacg ctcggcgcgt aactggagaa cagcgttttc ggtcataaga
     6841 gagcgtcgaa aaaacgccat tgtcgcagag gagaaaacgg ggcacaagat gcaccccggt
     6901 aagattaaag agattcgttc agccagctat cgaacggcgc tttcggtact gcgccattaa
     6961 gcatgtcgac aacctgaccg tttttgaaaa tcatgatcgt cggtatacta cgaattccaa
     7021 agcgactgct caattcacgt tcagcttcgg tattcacttt cacaaagcgc actttaccgc
     7081 tacgctcttg cgcgacatct tcaaaaattg gtgcgaaatt acggcagggg ccgcaccacg
     7141 gtgcccagaa gtcgatcacc acaggtagat catccttcag caatttgtcg agtgtttcac
     7201 cggtcgcatt aatcacctct ccgtcaaaca agtcgtgacc gcagcgtccg cattttgccg
     7261 catcttcgat ccgatcgtcg ggaatgcgat tgatggcctg acaatgggta caaacggtat
     7321 tcataactaa cctcgggatg agtaaggaga cttatcggca acgacgccgg tatgtttcta
     7381 atatgttaca tattatcggg gacattgttc cagacaacaa tctgttttat tcaataggta
     7441 tgatagtcgc aggctttggt acgaaatcat ggcgaaccgc gcggacttcg ggtaatctgc
     7501 gcgcttcgcg cagcgctggt ggagaaaagc atgaacgatg aaatgaaagg taaaagcggc
     7561 aaggtcaaag tgatgtatgt ccgcagtgat gatgattctg ataaacgtac ccacaacccg
     7621 cgtaccggga aagggggcgg gcgtccagga aaatctcgtg ctgacggtgg ccgtcgcccc
     7681 gcccgcgatg acaaacagag tcagccccgt gaccgcaagt gggaagattc gccgtggcgc
     7741 acggtttccc gcgcgccggg tgatgagacg ccggaaaagg ccgatcacgg tggcatcagt
     7801 ggtaaaagtt ttattgatcc ggaagtgttg cgtcgtcagc gtgcggaaga aacccgcgtc
     7861 tacggcgaaa atgcctgtca ggcactgttc cagagccgtc cggaagcaat tgttcgcgcc
     7921 tggtttatcc agagcgtaac gccacgtttt aaagaagcct tgcgctggat ggcggcaaac
     7981 cgcaaagcgt accatgtggt ggatgaagct gaattgacaa aagcgtcagg cacggaacat
     8041 cacggcggcg tttgcttcct gatcaaaaag cgtaacggta caaccgtgca gcagtgggta
     8101 agccaggcag gcgcgcagga ttgcgttctg gcactggaaa atgaatctaa cccgcataac
     8161 ctgggtggca tgatgcgcag ctgcgcgcac ttcggcgtga aaggtgtcgt ggtgcaggat
     8221 gcggcactgc tggagtcggg ggcggctatc cgtaccgcag aaggcggcgc agagcacgtt
     8281 cagccgatta ctggcgacaa cattgttaac gtgctggatg atttccgtca ggcaggttac
     8341 accgtagtga ccacttccag cgagcagggt aaaccgctgt tcaaaactag tctgccagcg
     8401 aaaatggtac tggtgctggg tcaggaatat gaagggttac cggatgccgc acgcgatccg
     8461 aacgatctgc gcgtgaagat tgacggtacg ggcaacgttg ccgggctgaa tatctctgtc
     8521 gcaaccggcg ttttgcttgg tgaatggtgg cgtcagaata aagcctgata aatcagccgg
     8581 gtggcaactc tgccatccgg catttccccg caaatttact cactctctgc cggtaatact
     8641 ggcatccagt caatcggcgt ctcgccacgt tgttccagcc actgatttgc cagcacaaaa
     8701 tggttgcagc caaagaatcc acgatgcgcc gaaagcggcg acggatgcgg tgctttcagt
     8761 acatgatggc gttgcttatc tataatcgcc cctttctttt gcgcatgtga tccccacaac
     8821 aaaaacacca cgccttcgcg gtgctggtta atcaggctga tgactttatc agtaaacgtc
     8881 tcccaaccga ggctggcgtg ggaatgcgcc tgacctgcgc gaaccgtcaa cacagtattg
     8941 agcagcagaa cgccctgacg cgcccagctt tcgagatagc catgattagg gcgggtgaag
     9001 cccggaatag tattttccag ctctttatac atattcaata atgacggagg aatggcaatg
     9061 ccgggacgaa cggaaaatgc cagaccatgc gcctgtcccg gtccgtgata aggatcctgg
     9121 ccgagaatca ccactttaac gtcacccaac tcggtaaagc ggaacgcgtt aaagacatct
     9181 ttttgtggtg gatagatagt gacgccggac tgccgctcgc tggcgacggt ctgaagggta
     9241 ttaagaaaat aaggttgttg cttctcttca gccagcacgt catgccaggt taattcgtta
     9301 gccatctcgc tctcctgcga atcttcaatc cgcctagctt aactgcttct ttctacagaa
     9361 caaaatcatg ctcatttttt agacttaaac gagtgcaaaa aaatgtaaat tggtaaaatt
     9421 tcacacccgc ataagttgat gtaaaacaat aaaatccatt tacgaccaca atcgatgtgt
     9481 ggtttttatt gatttaaatc aaagaatcaa gggtggttga ggagtatata tacactcaag
     9541 caacaatggt tttaccaatt ggccgcgaca ggctgaacaa atcaaataat tttgccgggg
     9601 aggcatcaca tgattacagg tatccagatt actaaagccg ctaacgacga tctgctgaac
     9661 tctttctggc tgctggacag cgaaaaaggc gaagcacgtt gcatcgttgc aaaagcaggt
     9721 tatgcagaag atgaagtggt tgcagtaagc aaactgggtg acattgaata ccgtgaagtt
     9781 ccagtagaag tgaaaccaga agttcgcgtt gaaggtggtc aacacctgaa cgttaacgtt
     9841 ctgcgtcgtg aaactctgga agatgcagtt aagcatccgg aaaaatatcc gcagctgacc
     9901 atccgtgtat ccggttatgc agttcgcttt aactctctga ctccggaaca gcagcgcgac
     9961 gttatcgctc gtacctttac tgaaagcctg taaggcttaa gtagcaagtt actaagcgga
    10021 agagggcgac ctcttccgct ttttttcgtt aatagaaaat gcgtaccgcg caatagacca
    10081 gtaacagggc aagcacgata tttaactggc gaccatactg gcgaaacaat cgctgaaaca
    10141 gatgccccgc cagcgcccag cacacattgc caaacgtccc aatcatcgcc agcaaaacgc
    10201 tgacgccaac tacccagctt agcgcctgtg tttgcggcag aacaaacgtc gacagtgccg
    10261 taacaccgta caaaatgatt ttgacgttca caaactgcaa agcaaagctg gcccaaaagc
    10321 tgattggttt tgcctgaagt ccgtcttcct ttgttgggct ggtggcgatt ttccacgcca
    10381 gccagacaat atatgccgcc cccgcccaac tcaaaaggtg taccgctgcc gggtcaatca
    10441 ctgccagtga aaatgaaatg cccgcacaca gtaacatcac aatcaaaaat cccagactca
    10501 tccctgccag cacgcgggta ctttgacgaa atccatgcga cgtagcagag ctaagggcga
    10561 gaatattgtt cggtcctggc gtcatagcgg taatcagggt gtaagtccaa aaagcactta
    10621 aaagggtcgg tgtcacttgt tatctcctct gttttggtag acagtacccg acacggaaca
    10681 tttaaaaaaa tgaatgtttt tatattctag attcgatatt ttcgataggt ttggggttat
    10741 ggatctgcgc cgttttatta cgcttaaaac cgtggtggaa gagggttcct ttttgcgagc
    10801 ttcgcaaaaa ttgtgctgta cacaatcgac ggtgactttt catattcagc agcttgagca
    10861 ggagttttca gtccagttat ttgagaaaat tggccgacgg atgtgcctta cccgcgaagg
    10921 aaaaaagttg ctgccgcaca tttatgaact tacccgagtg atggatacgc ttcgtgaggc
    10981 tgccaaaaag gagtcagatc cggacgggga actacgcgtt gtttcgggcg aaacgttgct
    11041 ctcttaccga atgccgcagg tgttgcagcg ttttcggcaa cgcgcaccaa aagtgcgttt
    11101 atcgttgcag tcgctgaact gttacgtgat ccgtgatgcg ctactgaatg atgaggccga
    11161 tgtcggcgtc ttttatcgcg tagggaatga tgatgccctg aaccgacgag agttgggtga
    11221 acaatcgctg gtgctggtgg cttcaccaca aattgtagat gtcgatttta ctgagccggg
    11281 aagacataac gcctgtagct ttattatcaa tgaaccacaa tgtgtcttcc ggcagatatt
    11341 tgagagcacg ctgcgtcagc ggcggatcac ggtggaaaac accattgagc ttataagtat
    11401 cgaaagcatc aagcgttgtg tcgcggcgaa tatcggagtc agctacctgc cgcgttttgc
    11461 ggtggcaaaa gagttggagt gcggagaatt aattgaattg ccttttggcg aacagtcgca
    11521 gaccattacg gccatgtgcg cacaccatgc tggaaaagcg gttagcccgg cgatgcacac
    11581 ttttattcag tgtgttgaag agagttttgt ggctggataa aaataatgtc gggattaaac
    11641 ccggcattta acttgtcgac aaagtgcgct ttattcatgc cggatgcggc gtgaacgcct
    11701 tatccggcct acaagatcgt gcaaattcaa tatattgcat gagccacgta ggcctgataa
    11761 gcgtagcgca tcaggcagtt ttgcgtttgc cagcactttt aataccaggc ttaaacccgg
    11821 cattgagatt actcttctgt cgtttgcggt ggcacgcccg ttccgcttgg tttacggcgc
    11881 ttaccaatat ttttggtgtc gcgatggcgt tttttcaccc gcggtttctc tttctcttta
    11941 gcttttttct tctcggcgcg ttttgccagt actttcttcg atggcttgcc ggtctgcttc
    12001 tcgcttggcg cacgcgtttt cgggcgtaac tcatcaataa cgcgagcttt aatcggctct
    12061 tcaatgtaac ggccaacctt gcccagcagc aaatggtcat gggcttccac cagcgaaatt
    12121 gcggtgcctt tacgaccggc gcgcgcggta cgtccgatac ggtgcaaata agtatcgcca
    12181 ctgcgcggca tatcgaagtt aaagacgtgg ctgacgtcag gaatgtcgat accgcgcgcg
    12241 gcaacatcgg ttgcaaccag tacgtttacg cgaccttcgg tcaaacgctt gatcgcttcg
    12301 ttacgcttgc cttgtaccat ctcaccttcg agatagcagt tgttgatgcc cgcttcgcgc
    12361 agccagtttg ccagctcatg cacacgttca cgcttacgca caaacacaat tgagcgggtc
    12421 gcttccggct gttttaacag atgcaccagc aatgcggttt tatgttccag gtcatcggcg
    12481 cggtaatacc actgatgaat ttttttgcgc tcacgggtgg acggattggc agaaacctcc
    12541 accggatctt ccagcagacg ctcggcaaag tcctgaatcg catcgccttc cagcgttgcc
    12601 gaaaagagca gggtctgttt acgccagcgc gtttcgccag caatatgttc gatatcctga
    12661 gcgaagccca tatccagcat acggtctgct tcgtcgagga tcagcgtttc aaccgcgcgg
    12721 caatcgaagt tctcttcttt tatgtattgc agcagacgtc cggtcgtggc gaccacgatg
    12781 tcctgatttt cgctgaacac ttccgcgtgg ttcatatagg ctacgccgcc ggtgatggtg
    12841 gcgatatcca gatgcgtatg tttcgccagt tcgcgggcat gatcggccac ctgcatcgcc
    12901 agctcgcgag ttggggtgag gatcaaaata cgcggcggac cggatttctt acgtgggaaa
    12961 tcgagcaggt gctgcaacgc tggcagcaga tacgccgccg ttttaccggt gcctgtcggc
    13021 gcagaaccga gtacatcacg gccatcgagc gcaggcggaa tggcggcagc ctgaatggcg
    13081 gtcgggcgag tgaaaccttt atcctggagg gcttccagca ggctttcgtc gagttcaagt
    13141 tcggaaaaag tcgttacagt catgttctac ctctgtgtgg ggcgctgatt atagacgtta
    13201 cggctgcaat cttcatctgt ttgtatggat atcgcttctc gggtattgtt ctcacccggt
    13261 gctatcgccg tttttcctgc aaggttttat tttcatgtca cagtctacat ccgtgcttcg
    13321 tcgtaatgga tttactttta aacagttttt tgttgctcac gatcgctgtg cgatgaaagt
    13381 gggaacggat ggcattttgc tgggcgcatg ggcaccggtg gctggggtga aacgttgcct
    13441 tgatatcggc gcgggtagcg ggttgctggc actgatgctg gcgcagcgaa ccagtgacag
    13501 cgtgataatt gatgccgttg agctggaaag tgaagctgcc gcgcaggcgc aggaaaatat
    13561 caaccagtcc ccgtgggcag agcggattaa cgtccatacg gcggatattc agcagtggct
    13621 cacacagcag acagtacgct tcgatttaat catcagtaac ccaccttact atcagcaggg
    13681 agtagagtgc gcgacacctc aacgggaaca ggctcgctat accactacgc ttgatcaccc
    13741 atcgctgctg acctgtgcgg cggagtgcat taccgaagag ggatttttct gcgtggtgct
    13801 gccagagcag gttggtaatg gttttactga actggcatta agcatgggct ggcatttacg
    13861 tttgcggacg gatgtggcgg aaaacgaagc gcgactgccg catcgggtgc tgctggcatt
    13921 ctccccgcag gcgggagaat gctttagcga tcgcttagtc attcgtgggc cagaccagaa
    13981 ctattccgaa gcgtatacgg cgctgaccca ggctttttat ctgtttatgt aatgattgcc
    14041 ggggggaagg atcgatggac cggaatgcgt gagcagttcc gggtaatcca gcgtaaaatg
    14101 caggccacga ctctccttgc gcatcatagc acagcgaaca atcaactcgg caacctgtac
    14161 cagattacgc agctccagca aattattcga cacgcggaag tgggcgtaat attcgtctat
    14221 ttcctgttgc aacatattga tacgccgcag ggcgcgctca agacgttttg ttgtgcgcac
    14281 aatgccgacg taatcccaca taaacagccg tagctcgtgc cagttatgct gaattactac
    14341 ccgttcgtca gggttctcaa cgcggctttc atcccacggt ggtaacgtac tgacgccgtg
    14401 ggcatcaggc atacgtctgg tgatatcttc tgccgccgac cagccataga ccagacactc
    14461 cagcaatgaa ttcgaggcca tgcggttagc gccgtgtaag ccggtataac tcacctcgcc
    14521 aatggcatac aagccctcga cgtccgtacg cccatgatca tcaaccatta caccaccgca
    14581 ggtatagtgt gcagcaggca caatcggtac cggttcttgt gtgagatcaa tccccagccc
    14641 gagcaatttt tcataaatca tcgggaaatg ctggcgaata aaatcggcgg gcttatggct
    14701 gatgtcgagg aacatacaat ctgcgccgag gcgtttcatt tcatggtcaa tggcgcgggc
    14761 gacaatatcg cgcggggcca gttcgccgcg ctcatcaaaa tcgggcataa aacgcgtgcc
    14821 atccgggcgc ttgagataag cgccttcgcc gcgcagtgct tccgttaaca ggaaattgcg
    14881 tgcctgtggg tgatacagtg cggtaggggg gaactgatta aattcgagat tggcaacccg
    14941 actgaggtgt actggcaata gcgga
//
LOCUS       DADMKF010000100        14582 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.102, whole genome shotgun sequence.
ACCESSION   DADMKF010000100 DADMKF010000000
VERSION     DADMKF010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14582
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.102"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            75..2216
                     /gene="ldcC"
                     /locus_tag="JNC24_002784"
     CDS             75..2216
                     /gene="ldcC"
                     /locus_tag="JNC24_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HAY9159488.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTNEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVIFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVEMAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECWPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLPGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            2273..2662
                     /locus_tag="JNC24_002785"
     CDS             2273..2662
                     /locus_tag="JNC24_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAY9159489.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            2727..4025
                     /gene="tilS"
                     /locus_tag="JNC24_002786"
     CDS             2727..4025
                     /gene="tilS"
                     /locus_tag="JNC24_002786"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HAY9159490.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVRHCENICQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMGEVSEFAGTRLIRPLLARTR
                     GELEQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSAALCAE
                     QESLLDELLADDLAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRFGAFEIRRYQSQLWWIKSVTGQSETIVPWQTWLQPLELPA
                     GLGSVQLTAGGDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(4074..4334)
                     /gene="rof"
                     /locus_tag="JNC24_002787"
     CDS             complement(4074..4334)
                     /gene="rof"
                     /locus_tag="JNC24_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HAY9159491.1"
                     /translation="MSMNDAYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(4321..4521)
                     /locus_tag="JNC24_002788"
     CDS             complement(4321..4521)
                     /locus_tag="JNC24_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HAY9159492.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            4687..5232
                     /locus_tag="JNC24_002789"
     CDS             4687..5232
                     /locus_tag="JNC24_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HAY9159493.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADYEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAKV
                     ALFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            5229..5590
                     /gene="arfB"
                     /locus_tag="JNC24_002790"
                     /pseudo
     CDS             5229..5590
                     /gene="arfB"
                     /locus_tag="JNC24_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308220.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
     gene            5664..6374
                     /gene="nlpE"
                     /locus_tag="JNC24_002791"
                     /gene_synonym="cutF"
     CDS             5664..6374
                     /gene="nlpE"
                     /locus_tag="JNC24_002791"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706138.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HAY9159494.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIETSLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
     gene            complement(6529..7353)
                     /locus_tag="JNC24_002792"
     CDS             complement(6529..7353)
                     /locus_tag="JNC24_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HAY9159495.1"
                     /translation="MDKPKAYCRLLLPSFLLLSACTVDISQPDPSATAVDAEAKTWAV
                     KFQHQSSFTEQSIKEITVPDLKPGDLLFSSSLGVTSFGIRVFSTSSVSHVAIYLGDNN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATDITAFANKIKDSSYNYRGIV
                     EFIPFMVTRQMCSLNPFSEDFRQQCVSGLAKAQLSSVGEGDKKSWFCSEFVTDAFAKA
                     GHPLTLAQSGWISPADLMHMRTGDVSAFKPETQLQYVGHLKPGIYIKAGRFVGLTQ"
     gene            complement(7406..9124)
                     /gene="proS"
                     /locus_tag="JNC24_002793"
     CDS             complement(7406..9124)
                     /gene="proS"
                     /locus_tag="JNC24_002793"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HAY9159496.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(9235..9942)
                     /gene="tsaA"
                     /locus_tag="JNC24_002794"
     CDS             complement(9235..9942)
                     /gene="tsaA"
                     /locus_tag="JNC24_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HAY9159497.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIAPYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(9939..10343)
                     /gene="rcsF"
                     /locus_tag="JNC24_002795"
     CDS             complement(9939..10343)
                     /gene="rcsF"
                     /locus_tag="JNC24_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HAY9159498.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKRMQINASKMKAN
                     AVLLHSCEVTSGTLGCYRQAVCIGSALNITAK"
     gene            complement(10461..11276)
                     /gene="metQ"
                     /locus_tag="JNC24_002796"
     CDS             complement(10461..11276)
                     /gene="metQ"
                     /locus_tag="JNC24_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HAY9159499.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(11316..11969)
                     /gene="metI"
                     /locus_tag="JNC24_002797"
     CDS             complement(11316..11969)
                     /gene="metI"
                     /locus_tag="JNC24_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HAY9159500.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(11962..12993)
                     /gene="metN"
                     /locus_tag="JNC24_002798"
     CDS             complement(11962..12993)
                     /gene="metN"
                     /locus_tag="JNC24_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HAY9159501.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQAEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            13181..13756
                     /gene="gmhB"
                     /locus_tag="JNC24_002799"
     CDS             13181..13756
                     /gene="gmhB"
                     /locus_tag="JNC24_002799"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HAY9159502.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPAQ"
     gene            14120..>14582
                     /locus_tag="JNC24_002800"
     rRNA            14120..>14582
                     /locus_tag="JNC24_002800"
                     /product="16S ribosomal RNA"
BASE COUNT         3563 a         3663 c         3893 g         3463 t
ORIGIN      
        1 cacttcggtg gccctttttt atcgccacgg tttgagcagg ctatgattaa ggaaggattt
       61 tccaggagga acacatgaac atcattgcca ttatgggacc gcatggcgtc ttttataaag
      121 atgagcccat caaagaactg gagtcggcgc tggtggcgca aggctttcag attatctggc
      181 cacaaaacag cgttgatttg ctgaaattta tcgaacataa ccctcgaatt tgtggcgtga
      241 tttttgactg ggatgagtac agtctcgatt tatgcagcga tatcaatcaa cttaatgaat
      301 atctcccgct ttatgccttc atcaacaccc actcgacgat ggatgtcagc gtgcaggata
      361 tgcggatggc gctctggttt tttgaatatg cgctggggca ggcggaagat atcgccattc
      421 gtatgcgtca gtacaccaac gaatatcttg ataacattac gccgccgttc acgaaagcct
      481 tgtttaccta cgtcaaagag cggaagtaca ccttttgtac gccggggcat atgggcggca
      541 ccgcatatca aaaaagcccg gttggctgcc tgttttatga ttttttcggc gggaataccc
      601 tcaaggctga tgtctctatt tcggtcaccg agcttggttc gttgctcgac cacaccgggc
      661 cacacctgga agcggaagag tacatcgcgc ggacttttgg cgcggaacag agttatatcg
      721 ttaccaacgg aacatcgacg tcgaacaaaa ttgtgggtat gtacgccgcg ccatccggca
      781 gtacgctgtt gatcgaccgc aattgtcata aatcgctggc gcatctgttg atgatgaacg
      841 atgtagtgcc agtctggctg aaaccgacgc gtaatgcgtt ggggattctt ggtgggatcc
      901 cgcgccgtga atttactcgc gacagcatcg aagagaaagt cgctgccacc acgcaggcac
      961 aatggccggt tcatgcggtg attaccaact ccacctatga tggcttgctc tacaacaccg
     1021 actggatcaa acagacgctg gatgtcccgt cgatccactt cgattccgcc tgggtgccgt
     1081 acacccattt tcatccgatc taccagggga aaagtggtat gagcggcgag cgtgttgcgg
     1141 gaaaagtgat cttcgaaacg caatcgaccc acaaaatgct ggcggcgtta tcgcaggctt
     1201 cgctgatcca cattaaaggc gagtatgacg aagaggcgtt taacgaagcc tttatgatgc
     1261 ataccaccac ctcgcccagt tatcccattg ttgcttcggt tgagatggcg gcggcgatgc
     1321 tgcgtggtaa tccgggcaaa cggctgatta accgttcggt ggagcgggcg ctgcattttc
     1381 gcaaagaggt ccagcggttg cgggaagagt ctgacggttg gtttttcgat atctggcaac
     1441 cgccgcaggt ggatgaagcc gaatgctggc ccgttgcgcc aggcgaacag tggcacggct
     1501 ttaacgatgc ggatgccgat catatgtttc tcgatccggt taaagtcact attttgacac
     1561 cggggatgga cgaacagggc aatatgagcg aggaggggat cccggcggcg ctggtggcaa
     1621 aattcctcga cgaacgtggg atcgtagtag agaaaaccgg cccttataac ctgctgtttc
     1681 tctttagtat tggtatcgat aaaaccaaag caatgggatt attgcgtggg ttgacggaat
     1741 ttaaacgctc ttacgatctc aacctgcgga tcaaaaatat gctacccgat ctctatgctg
     1801 aagatcccga tttctaccgc aatatgcgta ttcaggatct ggcgcaaggg atccataagc
     1861 tgattcgtaa acacgatctt cccggtttga tgttgcgggc attcgatact ttgccggaaa
     1921 tgatcatgac gccacatcag gcatggcaga gacagattaa aggtgaagta gaaaccattg
     1981 cgctggaaca actggtcggt agagtatcgg caaatatgat cctgccttat ccaccgggcg
     2041 taccgctgtt gatgccagga gaaatgctga ccaaagagag ccgcacggta ctcgattttc
     2101 tactgatgct ttgttccgta gggcaacatt accccggttt tgaaacggat attcacggcg
     2161 cgaaacagga cgaagacggc gtttaccgcg tacgagtcct aaaaatggcg ggataacttg
     2221 ccagagcggc ttccgggcga gtaacgtgct gttaacaaat aaaggagacg ttatgctggg
     2281 tttaaaacag gttcaccata ttgcgattat tgcgacggat tatgcggtga gcaaagcttt
     2341 ctactgcgat attcttggtt tcacgctgca aagcgaagtc tatcgcgaag cgcgcgactc
     2401 gtggaaaggg gatttggcgc ttaatgggca atatgtgatt gagcttttct cttttccgtt
     2461 cccgccggag cgccccagcc gaccggaagc ttgcggcctg cgtcatctgg cttttagcgt
     2521 tgatgatatc gatgcggcag tggcgcacct tgaaagccat aacgtgaagt gtgaagccat
     2581 ccgtgtcgat ccatacacgc aaaaacgctt cacgttcttt aacgatccgg acgggctgcc
     2641 gttggaactg tatgagcagt aaggcttgtc atcgccgcat ttgcccggta acgtgccggg
     2701 cattgctact gtaaaatcgc accatcatga cactcacgct caatagacaa cttctcacct
     2761 cacgccagat tctggtggcc tttagcggcg ggcttgactc caccgttctg ctgcatcagt
     2821 tggtgcagtg gcggacggaa aatccgggcg tcactctacg cgctatccat gtgcatcatg
     2881 gtttaagtgc taatgccgat gcctgggtta ggcattgcga gaacatctgc caacagtggc
     2941 aggtgccgct ggtggtcgag cgcgtgcaac ttgcgcaaga aggactgggc attgaggccc
     3001 aggcgcggca ggcgcgttat caggcatttg ctcgtaccct gttacccggt gaagtgctgg
     3061 tcaccgcgca acatctcgac gatcaatgtg aaacctttct gctggcgcta aaacgcggta
     3121 gcggccctgc cgggctttcg gctatggggg aagtttcgga atttgccgga acacggctta
     3181 tccgcccgtt gcttgcccgc acgcgggggg aactggagca gtgggcattg gcgcatggtt
     3241 tacgctggat tgaagacgaa agtaatcagg acgactcata cgatcgtaac tttctgcgcc
     3301 tgcgtgtagt gccgttattg cagcagcgtt ggccgcattt tgccgaagca acggcccgca
     3361 gcgccgcact ttgtgctgaa caagagagtc tgttggatga actgctggcg gatgatttag
     3421 cacactgcca aacgtcgcag gggacgctgc agattgcgcc aatgctggcg atgagtgatg
     3481 cccgtcgcgc ggcgattatc cgccgctggc tggcagggca gaatgcaccg atgccttccc
     3541 gcgacgcgct ggtgcggatc tggcaggaag tggcgctggc gcgggaagat gcctccccct
     3601 gtttacgttt tggcgcgttt gaaatacggc gctatcagtc gcaactgtgg tggattaaat
     3661 ccgtcactgg gcaaagcgaa accattgtgc cgtggcagac gtggctacaa ccgctggaat
     3721 taccggcggg gctgggaagt gtacagctta ctgcgggagg cgatattcgc cctccgcgtg
     3781 cagacgaagc ggtcagcgtg cgtttcaaag cgccaggatt gctgcatatt gtcgggcgta
     3841 acggcggacg taagctaaag aaaatctggc aagagctggg cgtgccgccg tggctacgtg
     3901 acaccacgcc actgctgttt tatggcgaaa cgctgattgc ggcggcaggg gtatttgtga
     3961 cgcaagaagg tgtggctgaa ggtgagaatg gcgtaagttt tgtctggcag aaaacgctta
     4021 gttaagtgaa agccggataa gacgcatcaa acgtcgcatc cggcgaaagt caatcaggat
     4081 tcgcttacca ccaccgtacc gatttccggg tggctaaagc tggtaatttt atccagacgc
     4141 agctcgcggg tttcgccagc ggcctcgacg accagatact ccacattttt gcgggagact
     4201 aaatcactgg cttttgcctg caatttttcg ccatctttca gctcaagtgt cagcattaaa
     4261 tgatgctggc aggcgagctc aagattatcg taatcatcac aattgattgg ttgatacgca
     4321 tcattcattg acataatcgc tcaccagtaa gtttgccgca gcgtatgctg ctttttccct
     4381 gacagcctca gaaagggcgt cgtcggcagc catttcattc agcactttca aaacgcagcc
     4441 cagcgcgtcc ggaacgtatc ctaagtctcc gctggcgatt tccgcgtacc gcttgcgtat
     4501 taactcacaa tatttttcca catgccctcc tgtcagcact ctgacttaac cgtggatgca
     4561 agtctaagcc tacgaagata aactctgttt cgcaaggtga ctataccaca ctcatttctg
     4621 caatatcagc gccgcaactg cacgtattcc gttacaatgg cctcctgatt cgaaaggagt
     4681 tttcttatgg cgcttaaagc gacaatttat aaagcgacgg ttaatgtggc cgatctcgac
     4741 cgcaaccagt ttctcgatgc ctctctgacg ctggcgcgcc acccttcaga aacccaggag
     4801 cgtatgatgc tgcgcctgct ggcgtggctg aaatatgctg atgaacgtct gcaatttacc
     4861 cgtggtttgt gtgccgatta tgagccggaa gcgtggctgc gtaacgatca cctgggcatt
     4921 gatttgtgga ttgagctggg gctgccggat gagcggcgga ttaagaaagc ctgcacccag
     4981 gccgcaaaag tggcgctgtt tacctataat agccgggcgg cgcaaatctg gtggcagcaa
     5041 aatcagagca aatgtgtgca gtttgccaat ctttccgtct ggtatctgga cgatgaacaa
     5101 ctggcgaaag taagcgcctt tgccgatcgt accatgacgc tgcaggcaac gattcaggat
     5161 ggcgtgatct ggttatcgga tgataagaat aatctggaag tgaacttaac cgcctggcaa
     5221 caaccttcat gattgtgatt tcccgacatg ttgctattcc cgatggtgag cttgagatca
     5281 ccgccattcg tgcgcagggc gcgggcgggc agcatgttaa taagacctca acggctattc
     5341 atctgcgttt tgacattcgg gcgtccagcc tgccagagta ttacaaagag cgtctgctcg
     5401 ccgccagcca tcatttgatc agcatgatgg cgtaattgtc attaaggcac aggaataccg
     5461 cagtcaggaa ctgaaccgcg aagcggcgct ggcccggctg gtggcagtga ttaaagattt
     5521 aacaacagaa caaaaagccc gacgacccac gcggcccacc cgtgcatcga aagagcgcag
     5581 gctggcatag aaagcacaaa aatcaagcgt gaaggcgatg cgcggcaaag tacgcagcgg
     5641 tcgggaataa aaagaaggaa tggatggtga aaaaagcgat agtgacagcg atggctgtaa
     5701 tcagcctctt tactctgatg ggatgtaata atcgggccga agtcgatacg ctttctccgg
     5761 cgcaggctgc cgaactaaaa ccgatgccgc aaagttggcg cggcgtgctg ccgtgtgccg
     5821 attgcgaagg gatcgaaacc tctctgttcc tcgaaaaaga cggaacatgg gtgatgaatg
     5881 agcgttatct cggtgctcgt gaagaacctt cctccttcgc ttcctacggt acatgggcgc
     5941 gaaccgctga caagctggta ttaaccgaca gcaaaggtga aaagtcatat tatcgggcga
     6001 aaggcgatgc gctggagatg ctcgatcgtg aaggcaatcc aattgaatcg cagttcaact
     6061 atacgctgga accggcacaa tccagtttac ctatgacgcc gatgaccctg cgaggcatgt
     6121 atttttatat ggctgatgcg gcgaccttca ctgattgcgc gaccggaaaa cgtttcatgg
     6181 tagcgaataa cgcagagctg gagcgtagct acctggctgc gcgcggtcac agtgaaaaac
     6241 cggtgttact gtcagtagaa ggtcacttta cgcttgaggc taatccggat actggtgcgc
     6301 caaccaaagt attagcgccc gatacggcgg gtaaatttta cccgaataag gattgcagta
     6361 gtttggggca gtaaacctgc ttaaggcaga aacaaacgca aaattgcctg atgcgctgcg
     6421 cttatcaggc ctacagcata taatgcaaca ttttgaatta gcacgatttt gtaggccgga
     6481 taaggcgttc acgacgcatc cggcattttg tgcacgaggc tggctttatc actgagtcag
     6541 cccaacaaag cgacccgcct tgatataaat ccctggcttc aggtgcccaa catactgtaa
     6601 ctgcgtttct ggcttaaacg cagagacatc gccagtacgc atatgcatca gatcggcagg
     6661 gctaatccag cctgattgcg ccagcgtaag cggatgcccg gctttggcaa atgcatcagt
     6721 gacaaattcc gaacaaaacc acgacttttt gtctccttcg cccacactgc ttaactgcgc
     6781 tttcgccagg ccgctgacgc actgttggcg aaaatcctcg gagaacggat tcagtgagca
     6841 catctggcga gtcaccataa agggaataaa ttcgacaatg ccgcgatagt tataactgct
     6901 atctttgatt ttattggcaa acgcggtgat atctgtggct tgttgcgggg taagatccgg
     6961 gactcgtaag acgaaaagct tatcactatg cttcatcgct tttttaaggg aaacgatctg
     7021 gacgccagcg cctgtcgctt ctgcaacgtt attatcaccc agatagattg caacgtgact
     7081 cacagaggaa gtgctgaaga cgcggattcc aaatgaggtt accccaaggc ttgaggagaa
     7141 cagcaaatcg ccgggtttga gatcaggtac cgttatttct tttattgatt gttcggtgaa
     7201 agagctttga tgctggaatt tgacagccca tgtttttgcc tcggcatcta ctgctgtggc
     7261 tgatgggtct ggctggctga tatcaacggt acaagccgaa agtaaaagaa agctggggag
     7321 aagcaggcgg cagtacgcct ttggtttatc cattttatac aatccatgta aaaaagggcc
     7381 ctgaaattca ggaccctttc tggcatcagc ctttaatctg tttcaccaga tattcgacga
     7441 tgtcaccagt cttaattaac tgtttctcgc cgttgcgacg atatttatat tcgatatcgt
     7501 cgttgtcgag gttacggtcg cccagcacaa tagtgtgcgg aataccgatc agttccatat
     7561 cagcaaacat cacgcccgga cgctctttgc ggtcatccag cagcacttcg ataccttgtg
     7621 cacgcagttc gctgtacagt ttctcagcaa gctcttgtac gcggaaggat ttgtgcatgt
     7681 tcatcggcag aatcgccacc tggaacggcg cgatagcgtc aggccatacg atgccgcgtt
     7741 cgtcgtagtt ctgctcaatc gccgcagcta ccacacgcgt taccccgata ccgtagcaac
     7801 ccatcgtcag gatttggtta cggccatctt caccctgtac ggaggctttc agtgcttcgg
     7861 agtacttggt acccagctgg aagatgtgac caacttcgat accacgtttg atcagcagcg
     7921 taccctggcc atccgggctt ggatcgccag ccaccacgtt acggatatct gcaacttccg
     7981 gggtagcgac atcgcgatcc cagttgatac cgaagtagtg tttaccatcg atgttagcac
     8041 cagcagcgaa atcactcatc gccgcaacgg tacggtcaat caccaccgga atcggcatgt
     8101 ttaccggacc cagtgaaccc ggaccggctt taaccacagc acgaatttct tcttcggtcg
     8161 cgaaagtcag cgggcttgca acctgcggca gtttttctgc tttaacttcg ttcagctcgt
     8221 gatcaccgcg caccagcagc gcaaccagcg ggaagctgct gccttcaacc gctttaacca
     8281 gcagagtctt aaccgttttc tcaatcggca gattgaactg ttcaaccagt tccgcgatgg
     8341 ttttcgcgtt cggcgtatca accagcgtca tttcctgggt agcagcagcg cgcggttctt
     8401 tcggcgcgat agcttctgcc agttcaatgt tcgctgcata gtcagaggtg tcggagaaga
     8461 ccacatcgtc ttcaccgctc tgcgccagca cctggaattc gtgagaggcg ctgccgccga
     8521 tagaaccggt gtcggcttgc acggcgcgga aatccagccc catgcggctg aagattttgc
     8581 tgtaggccgc atacattgca tcgtaggttt cctgcaggga ttcctgagaa gtatggaaag
     8641 agtaagcatc tttcatcagg aattcgcggg aacgcatgac gccgaaacgc ggacgcactt
     8701 cgtcgcggaa cttggtctgg atctgataga agttcagcgg cagctgtttg taagagctaa
     8761 gctcgttacg aatcaggtca gtgataactt cttcatgagt tgggccgagt acgaacggac
     8821 gctcgccacg gtcaacaaaa cgcagcagtt ccggaccgta ctgttcccaa cgaccactct
     8881 cttgccacaa atcggctggc tgaaccaccg gcatcgacac ctcgattgca ccggcgttgt
     8941 tcatctcttc acgcacgatg ttttcgactt ttttcagaac gcgcacgccg gtcggcagcc
     9001 aggtatataa cccggaggcc agcttgcgga tcatcccggc gcgcagcatc agctgatggc
     9061 tgatcacctc ggcgtcggca ggtgtctcct tgagagtgga gagcaggtat tggctagtac
     9121 gcatgttgtt acggttccag ttggaaggta gaacaggctc aaggcgagcc tgggacaaaa
     9181 aaagtgattt agtttaccag tgcaaaagaa atgtcaaaag agaagggcgt gaatttaacg
     9241 cggttccagc gcaaagactt caaaacctgc gtcggtgacg cgccagcgaa cgttaaaatc
     9301 atgtagccag acggcatagg ttttgcccgt ttcctcacct ttacgatagg ccgggcgcgg
     9361 gtcctgcgcc agtacttcgc ggataaacag cgttaactgc ggataacgct tctccagcgt
     9421 caaaagctgc ttttcgacct ctgcggtaaa actcaccgcc atctctgcag ctggcgcact
     9481 ttgcgcatag ctggcactgg catcgggaag cgattcggca aaggggagat acggtttgat
     9541 atccactacc ggcgtaccat cgaccagatc cagactgccg agcttcagaa tcacgctgtc
     9601 tttatggcaa acaacctctt tcagctctac cagcgacata ccaattgggt tagggcggaa
     9661 agtagagcgt gttgcgaaaa cccccattct ggcgttaccg ccgaggcgcg gtggacgcac
     9721 agtcggacgc cagccgcctt ccatcgtttg atgaaagacg aaaaggatcc ataaatggct
     9781 gaacgcttcc aggccgcgaa cggcgtcggc ctggttgtag ggagcaatga gatgcagttc
     9841 gccgttggcg ctttttacca gaccaggctg gcgcggaacg gcgaattttt ctttataggg
     9901 cgagcgaata acgcctattt gctcgaactg gaaactgctc atttcgccgt aatgttaagc
     9961 gcagagccga tacatacagc ctgacgatag cagcctagcg taccgctggt gacttcgcag
    10021 ctatgcagta ataccgcatt ggctttcatt ttagaggcgt tgatttgcat ccgcttacgc
    10081 gcggttggaa tgctcggcgg agagtcctga ttagaggcct ggcaagagtc gccactgact
    10141 tcaccgagat cgcggaacgg tttgccgact aattcttctg cattggtata aattcggacc
    10201 ggcgtggcgc gcggcgcttt cggttttgca ggctccgctt tcggctgggg tgcagtgctt
    10261 tgaacgggtt cgacagggga tctgcttaac atggaacagc cgcttagcat gagtgctact
    10321 aaacagatcg gtaaagcacg catagtattt cctcaatgta tgatcaaaac gtcaatattg
    10381 aatcaggagc ttgtaaaaat gacaagacgg gcaagcgccc gtcctgaatg atattacaaa
    10441 ttgtggaaac agcctaaaaa ttaccagcct ttaacagcgc cgccgttaaa cactttgttt
    10501 gctgcttcgt aaacttcatc agactgataa gcctggacga atttcttcac gttctcggcg
    10561 tctttgttat cttcacgcgt tacgatcagg ttcacgtacg gggagtcttt atcttcgaca
    10621 aagataccgt ctttcgctgg agtcaggcca atctggctgg cataggtggt attgataact
    10681 gccagagcga tttgcgcgtc gtccagagat cgaggcagct gcggtgcttc cagctcaaca
    10741 attttcagat tttttgggtt ctcaacaaca tcaagaacgg tcggcagcag gccaacgcca
    10801 tctttcagtt tgatcaagcc cactttttgc agcagcagca gtgaacgacc aagattagtt
    10861 ggatcgtttg gcacggcaac ctgcgaacca tcctgcagtt catccagtga tttgattttc
    10921 ttggagtaac cagcaatcgg ataaacaaaa gtgttgccta ctgcgaccag tttgtagcca
    10981 cgatctttca gttgctgatc aaggtacggt ttatgctgga aggcgttggc gtcgatatcg
    11041 cctttgctca atgcttcgtt tggcagaaca tagtcgttga aggttaccag ctcaacgtcc
    11101 aggccatatt tgtctttcgc aactttctgc gcgacttctg ctacctgctg ttcggcacca
    11161 acaatcacgc cgactttaat gtggtttgga tctttttcat cctgaccgca gcctaccagt
    11221 gccagtgatc caatcagggc tcccactgcc gcaaaggttt tgaatttgaa cgccatacct
    11281 tattccttct tcaattattt atgttgtgtt gaacgttact tgcgagtgac agcccggacg
    11341 atgcggtcgc ctgcgaactg aattaaataa accagaatga ccagcaatac cagtaccgta
    11401 ttcatcaccg tcgcgttgta gccgatgtag ccatactgat agccaatctg acctaaacca
    11461 ccggcaccga ctgcaccacc catcgcggaa taaccgacca gggtaatcag ggtgatagtt
    11521 gccgcattca ccagacccgg caacgcttcc ggtaacagca ctttacggac gatctgcatt
    11581 ggcgtggcac ccattgcgcg ggaagcttca attaacccgg ttgggatctc cagcagagcg
    11641 ttctcgacca tacgggcaat aaacggtgct gcaccaacgg ttaacggaac aattgctgcc
    11701 tgcaaaccaa tcgatgtacc gacaataacg cgggtaaacg gaatcatcca taccagcaag
    11761 ataatgaacg ggatggaacg gaaaatgttc acaatcgcag aaacggtacg atacagcttc
    11821 gcgttagcaa taatttgccc cggacgcgtg acataaagca gaacgccaac cggcagacca
    11881 atcacaaagc caaaaaatcc ggatacgaag gtcattgcca gcgtttccca tacgccacga
    11941 accagcagcc acatcatcgg ctcagacata acccagtacc tctactttta catggtgttc
    12001 ctgcagccag gcaatggcgg cttgcgtatc ttgttgtgtg ccgtgcattt cagtcagcat
    12061 gatgccgaat ttaacgccac cggcgtagtc catctgcgcg ctaataatgt tgttgttgac
    12121 gttgaaacga cgcgcggttt cagaaagcag tggggcatcg accgattgac cggtaaactc
    12181 cagacgcagc atcggcacgc agtcagtaaa tggctccgct tgcagacgtt cctggtaatc
    12241 ttccgggata tccagatgca gggtcgactg aataaacttc tgcgccagcg gcgttttcgg
    12301 atgcgagaac acttcactta ccgtgtcctg ctcgatcagt tcgccattgc tgatgaccgc
    12361 cacgcaatca caaatacgct tcacaacgtc catttcgtgg gtgatcaaca gaatcgtcaa
    12421 acccagacgg cggttaatgt ctttcagcag ttcgagaata gaacgtgtcg ttgccgggtc
    12481 cagcgcgctg gtggcttcat cacacagcaa tactttggga ttgctggcta atgcacgggc
    12541 aatcgccaca cgttgtttct gcccaccgga aagattcgac gggtagctat catgcttatc
    12601 gccaagacca accaatgaca gcaattccgt cacgcgacgt ttgatctcgt ctttcggtgt
    12661 gttgtccagc tccagcggca gagccacgtt gccaaaaaca gtacgcgaag agagcaggtt
    12721 aaaatgctgg aaaatcatac caatctggcg gcgagctttg gtcaactcgg attctgacag
    12781 cgtggtcaat tcttggccat cgaccagcac gctaccctcg gttgggcgct ccagcaggtt
    12841 tacacaacgt ataagcgtac tcttacccgc gcctgaggca ccgataacgc cataaatttg
    12901 tccagctggc acatgcaggc tgacgttgtt caacgcctgg atggtgcggg tgccctggtg
    12961 gaacactttg gtgatattcg aaagttttat cattgattat ttattatcgt cattaagtta
    13021 gtcgtggcat ctcgaatgcc tgaaacgggc aacgccgtca atgaaatgga tgttaaggca
    13081 tccagacgtc taaatcaatc aggtttatgc gaagagcact ttcttgcagg tcgaaacatg
    13141 cgatactagc gtcccatgcc ttattaagga gctataaaag gtggcgaaga gcgtacccgc
    13201 aatttttctt gaccgtgatg gcaccattaa tgtcgatcac ggctatgtcc atgagatcga
    13261 caactttgaa tttatcgacg gtgttattga cgccatgcgc gagctaaaaa aaatgggctt
    13321 tgcgctggtg gtagtaacca accagtctgg cattgctcgc ggtaaattta ccgaagcaca
    13381 gtttgaaacg ctgaccgagt ggatggactg gtcgctggcg gaccgagatg tcgatctgga
    13441 tggtatctat tattgcccgc atcatccgca gggtagtgtt gaagagtttc gccaggtctg
    13501 cgattgccgc aaaccacatc cggggatgct tttgtcagca cgcgattatt tgcatattga
    13561 tatggccgct tcttatatgg tgggcgataa attagaagat atgcaggcag cggttgcggc
    13621 gaacgtggga acaaaagtgc tggtgcgtac gggtaaacct attacacctg aagcagaaaa
    13681 cgcggcagat tgggtgttaa atagcctggc agacctgccg caagcgataa aaaagcagca
    13741 aaaaccggcg caatgattaa aagatgagcg gttgaaataa aaatgcattt ttccgcttgt
    13801 cttcctgagc cgactcccta taatgcgcct ccatcgacac ggcggatgtg aatcacttca
    13861 cacaaacagc cggttcggtt gaagagaaaa aatcctgaaa ttcagggttg actctgaaag
    13921 aggaaagcgt aatatacgcc acctcgcgac agtgagctga aagctgcgtc gcaactgctc
    13981 tttaacaatt tatcagacaa tctgtgtggg cactcgaaga tacggattct taacgtcgca
    14041 agacgaaaaa tgaataccaa gtctcaagag tgaacacgta attcattacg aagtttaatt
    14101 ctttgagcat caaactttta aattgaagag tttgatcatg gctcagattg aacgctggcg
    14161 gcaggcctaa cacatgcaag tcgaacggta acaggaaaca gcttgctgtt tcgctgacga
    14221 gtggcggacg ggtgagtaat gtctgggaaa ctgcctgatg gagggggata actactggaa
    14281 acggtagcta ataccgcata atgtcgcaag actaaagagg gggaccttcg ggcctcttgc
    14341 catcggatgt gcccagatgg gattagctag taggtggggt aacggctcac ctaggcgacg
    14401 atccctagct ggtctgagag gatgaccagc cacactggaa ctgagacacg gtccagactc
    14461 ctacgggagg cagcagtggg gaatattgca caatgggcgc aagcctgatg cagccatgcc
    14521 gcgtgtatga agaaggcctt cgggttgtaa agtactttca gcggggagga agggagtaaa
    14581 gt
//
LOCUS       DADMKF010000101        14371 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.103, whole genome shotgun sequence.
ACCESSION   DADMKF010000101 DADMKF010000000
VERSION     DADMKF010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14371
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.103"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(<1..698)
                     /locus_tag="JNC24_002801"
     CDS             complement(<1..698)
                     /locus_tag="JNC24_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAY9159503.1"
                     /translation="MNTLPEHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLIAGAGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHVQPNGEESYHLTREGGHSHRRILHAADATGREVQSTLVSKAQNHPNIRVLERS
                     NAVDLIVSDKIGLPGTRRVVGAWVWNRNKETVETCHAKAVVLATGGASKVYQYTTNPD
                     ISSGDGIAMAWRAG"
     gene            954..1109
                     /gene="rseD"
                     /locus_tag="JNC24_002802"
     CDS             954..1109
                     /gene="rseD"
                     /locus_tag="JNC24_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HAY9159504.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            1106..1681
                     /gene="rpoE"
                     /locus_tag="JNC24_002803"
     CDS             1106..1681
                     /gene="rpoE"
                     /locus_tag="JNC24_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HAY9159505.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            1714..2364
                     /gene="rseA"
                     /locus_tag="JNC24_002804"
     CDS             1714..2364
                     /gene="rseA"
                     /locus_tag="JNC24_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HAY9159506.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQPATLIPEAQPAPHQWHKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGLQTLGTQSQ"
     gene            2364..3320
                     /gene="rseB"
                     /locus_tag="JNC24_002805"
     CDS             2364..3320
                     /gene="rseB"
                     /locus_tag="JNC24_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HAY9159507.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVISFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            3317..3796
                     /gene="rseC"
                     /locus_tag="JNC24_002806"
     CDS             3317..3796
                     /gene="rseC"
                     /locus_tag="JNC24_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HAY9159508.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAILGGIGGFLIARGYSRKFAARAEWQPIILSVALPPDLVRFETSSEDASQ"
     gene            3994..5793
                     /gene="lepA"
                     /locus_tag="JNC24_002807"
     CDS             3994..5793
                     /gene="lepA"
                     /locus_tag="JNC24_002807"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010434076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HAY9159509.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGSLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            5809..6783
                     /gene="lepB"
                     /locus_tag="JNC24_002808"
     CDS             5809..6783
                     /gene="lepB"
                     /locus_tag="JNC24_002808"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAY9159510.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKLGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            7056..7736
                     /gene="rnc"
                     /locus_tag="JNC24_002809"
     CDS             7056..7736
                     /gene="rnc"
                     /locus_tag="JNC24_002809"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003848606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAY9159511.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            7733..8638
                     /gene="era"
                     /locus_tag="JNC24_002810"
     CDS             7733..8638
                     /gene="era"
                     /locus_tag="JNC24_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HAY9159512.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLRDGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            8650..9378
                     /gene="recO"
                     /locus_tag="JNC24_002811"
     CDS             8650..9378
                     /gene="recO"
                     /locus_tag="JNC24_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HAY9159513.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGATGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            9390..10121
                     /gene="pdxJ"
                     /locus_tag="JNC24_002812"
     CDS             9390..10121
                     /gene="pdxJ"
                     /locus_tag="JNC24_002812"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HAY9159514.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQREKMRDACKRLTDAGIQISLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELVRIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            10121..10501
                     /locus_tag="JNC24_002813"
     CDS             10121..10501
                     /locus_tag="JNC24_002813"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HAY9159515.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            10723..10803
                     /gene="shoB"
                     /locus_tag="JNC24_002814"
     CDS             10723..10803
                     /gene="shoB"
                     /locus_tag="JNC24_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HAY9159516.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(10997..11257)
                     /gene="yfhL"
                     /locus_tag="JNC24_002815"
     CDS             complement(10997..11257)
                     /gene="yfhL"
                     /locus_tag="JNC24_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HAY9159517.1"
                     /translation="MSLLITKRCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
     gene            11472..12674
                     /locus_tag="JNC24_002816"
     CDS             11472..12674
                     /locus_tag="JNC24_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAY9159518.1"
                     /translation="MPRTVTHNPDSPNNDDVLAASEKWDACKPPYTSAHMKICVAAAK
                     IILAASGVARRSKYEKENYLRIDFSKAGKVTFYAEFPKKMGLKGKKLGEWPELAIQLA
                     REKALGMADGGLRAESVHAALEMYRDDLKAKVARQKLSPDSFTTYGVRIDRIKATFGE
                     REVFSDVTYNRLVEVLDEWIATRSNNNALELFAELRRFWKFCAPTLCNGRNVAASLPD
                     DYVSSRVQKPTPTRLFTDIESIARLWLNVAACTSVHQKNAVRFMIITGVRPINVHNLR
                     WDYVYEEAGEIVYPEGVIGMRGAMKTQKAFRLPITPEIRRIIDEQKAWRDSVPECNRD
                     YVFLQPRDPMQPFSKRSLDKLVKTYSPDGAVKGIKHDGTVKGKDGAFNTMCRKFLKSN
                     VIALIDRN"
     gene            complement(12847..14034)
                     /locus_tag="JNC24_002817"
     CDS             complement(12847..14034)
                     /locus_tag="JNC24_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HAY9159519.1"
                     /translation="MLPRFADIFQQGNHWLNWLEKQPERAVRPVVIESVTKIMACGTT
                     LMGYTQWCCSSPDCCHTKKVCFRCKSRSCPHCGVKAGAQWIQYLLSLVPDCPWQHIVF
                     TLPCQYWSLVFHNRWLLTEMSRIAADVILEICHQADVEPGIFTVIHTWGRDQQWHPHI
                     HLSTTAGGVTSGHTWKNLHFYARKVMSMWRYRITRLLSRKYPDLVMPDALAAEGSSKR
                     EWNRFLDTHYRRGWNVNVSRVMDNAPHVAVYFGSYLKKPPVPMSRLEHYAGQDEIGLR
                     YNSHRTKREEYLLMSGDEFMERFSWHVADKGFRMVRYYGFLSPAKRRLLEEVVYIITE
                     TVRKTAMQITWRGMYQRLLKVDPLKCVLCGSQMRFTGLKRGYRLAEQVLMHEPLARMR
                     WCG"
     gene            complement(14034..>14371)
                     /locus_tag="JNC24_002818"
     CDS             complement(14034..>14371)
                     /locus_tag="JNC24_002818"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9159520.1"
                     /translation="KPASQRSKLPRYVVKLHEDDFFDEEDAEVLRFDSFDDAVECCAD
                     LNIPFFVDAGNKKLVFWFVRVDDEGYPEIARCTEREFATILSGISAGGMYCPECGTVH
                     WPDGVAPPF"
BASE COUNT         3622 a         3606 c         3708 g         3435 t
ORIGIN      
        1 cagcccgcgc gccacgccat agcaatgcca tcgccagaag aaatatccgg attggtggtg
       61 tactgataca ccttcgacgc accgccggtt gccagtacca ctgcttttgc gtggcaggtt
      121 tccaccgttt ctttatttcg gttccatacc cacgcgccta caacccgtcg cgtgcccggc
      181 aggccaattt tgtcagagac aatcagatca actgcgttgc tgcgctccag cacacgaata
      241 ttcggatggt tctgcgcctt gctcaccagc gtggattgta cttctctacc ggtggcgtcg
      301 gcggcatgaa gaatacgacg gtgactatgt ccaccttcac gggtcagatg gtagctttct
      361 tcgccattcg gttgaacgtg ggtatcaaac aaaaccccct ggtcaattaa ccattgcaca
      421 caggaacgag cattgctggc gacaaattca actgcatggc gatcgcaaat accagccccg
      481 gcaatcaatg tgtcttccac atgcgagtca atgctgtcag tttcatcaaa cacggcggca
      541 ataccgccct gggcataaaa tgttgaacct tccgttaccg ggcctttact tagaacgatg
      601 acctgatgct ggtcagccag gcgtagcgcc agtgaaagtc cggctgcgcc gctaccaata
      661 atcaacacgt cacatgaatg ttcagggaga gtattcattt tctttgttta atttactaaa
      721 cattgtttgg tcagcatagc atcatgttgt gcgaataaac acctgctatt ttaatatttg
      781 ttacagtggc taaacacgtt gacgcagggc ggcgagaaaa agagaagtta ctggctggtg
      841 gaggattagg tggtgaaata aaaaggccgt tgggttactc ttcaggcagt taaatgggca
      901 tttctacaca gataatgcga tgttcagatt ctgtagactt ataatgatag ataatgatcc
      961 gtctacagca tgacaaacaa aaacagatgc gttacggaac tttacaaaaa cgagacactc
     1021 taaccctttg cttgctcaaa ttgcagctaa tggagtggcg tttcgatagc gcgtggaaat
     1081 ttggtttggg gagactttac ctcggatgag cgagcagtta acggaccagg tcctggttga
     1141 acgggtccag aagggagatc agaaagcctt taacttactg gtagtgcgct atcagcataa
     1201 agtggcgagt ctggtttccc gctatgtgcc gtcgggtgat gttcccgatg tggtacaaga
     1261 agcttttatt aaagcctatc gtgcgctgga ttcgttccgg ggagatagcg ctttttatac
     1321 atggctgtat cggattgctg taaatacagc gaaaaattac ctggttgctc aggggcgtcg
     1381 tccaccttcc agtgatgtgg atgccattga agctgaaaac ttcgaaagtg gcggcgcgtt
     1441 gaaagaaatt tcgaaccctg agaacttaat gttgtcagaa gaactgagac agatagtttt
     1501 ccgaactatt gagtccctcc cggaagattt gcgcatggca ataaccttgc gggagctgga
     1561 tggcctgagc tatgaagaga tagccgctat catggattgt ccggtaggta cggtgcgttc
     1621 acgtatcttc cgagcgaggg aagctattga taacaaagtt caaccgctta tcaggcgttg
     1681 acgatagcgg gatactggat aagggtatta ggcatgcaga aagaacaact ttccgcttta
     1741 atggatggcg aaacgctgga tagtgagctg cttaacgaac tggctcataa cccagaaatg
     1801 cagaaaacct gggaaagcta tcacttaatc cgtgattcaa tgcggggtga tactcccgag
     1861 gtgctccatt tcgatatctc ttcacgcgtg atggccgcca ttgaagaaga gccagtacgt
     1921 caaccggcga cattgatccc ggaagcccag cctgcgccgc atcaatggca caaaatgcca
     1981 ttctggcaga aagtacgtcc gtgggcggca cagcttaccc aaatgggcgt agcagcatgc
     2041 gtatcgcttg cagttatcgt tggcgtccag cactataatg gacaatctga aacgtcccag
     2101 cagcccgaaa cgccggtatt taatacactg ccgatgatgg gtaaagccag cccggtaagc
     2161 ctgggagtac cttctgaagc gaccgcaaac aatggtcaac agcagcaggt acaggagcag
     2221 cgtcgtcgca ttaatgcaat gttgcaggat tacgaactgc aacgccgact ccactctgaa
     2281 cagcttcagt ttgagcaggc gcaaacccag caagccgctg tacaggtgcc aggacttcaa
     2341 actttaggaa cgcaatcgca gtaatgaagc aactttggtt tgccatgtca ttagtgacag
     2401 gtagcctgtt attctctgct aacgcctcgg ccactcccgc gtccggggcg ttattacagc
     2461 agatgaacct ggccagtcag tcactgaatt acgagctgtc attcatcagc atcaataaac
     2521 agggtgttga gtctctgcgt tatcgacatg cacgcctcga taaccgtcct cttgcacaat
     2581 tgttgcaaat ggatggcccg cgccgggaag tggtacagcg cggcaatgaa atcagctatt
     2641 ttgaaccggg acttgaaccg ttcacgctta atggcgatta cattgttgat tctctgccat
     2701 cgcttatcta taccgatttc aaacgccttt ctccttacta cgactttatc tccgtcgggc
     2761 gcacgcgtat tgctgatcgt ctttgcgaag tcattcgcgt ggttgcccga gatggtacac
     2821 gctacagcta catcgtgtgg atggacactg aatcgaaatt accgatgagg gttgatctcc
     2881 ttgatcgcga tggtgaaacg ctggaacaat ttcgcgtgat ttcttttaac gtcaatcagg
     2941 atatcagcag cagtatgcag acgctggcga aggcaaattt gccgccgttg ctttctgttc
     3001 ctgtaggtga aaaagctaaa ttcagctgga cgccaacctg gttgccacag ggttttagcg
     3061 aagtttccag tagtcgacgt ccgctaccga cgatggacaa catgcctatc gaatcacgtc
     3121 tctattccga cggattattc agcttctcgg taaacgttaa ccgcgctacg ccatcgagca
     3181 ccgatcagat gttgcgcacc ggacgcagaa ccgtcagtac aagcgtacgt gataacgccg
     3241 aaatcaccat tgtcggtgaa ctgccgccgc aaacggcgaa acgcattgcc gagaatatta
     3301 agttcggggc agcgcaatga tcaaagagtg ggctaccgtc gtctcctggc aaaacgggca
     3361 ggcgctggtc agttgtgatg ttaaagcctc atgcagcagc tgcgcttcac gcgccggttg
     3421 cggtagccgc gtgttgaata aacttggccc gcaaaccacg cataccattg tcgtaccctg
     3481 tgatgaaccg ttagtgccgg ggcaaaaagt ggaattgggg atcgccgaag gcagcctgct
     3541 tagctccgca ttactggttt atatgtcgcc gctggtggga ttattcctta tcgcttcgct
     3601 atttcagcta ctctttgctt ctgacgttgc agcattatgt ggtgcgatcc ttggcggtat
     3661 tggcgggttt ctgattgcgc gcggttactc gcgtaagttt gctgcccggg cagaatggca
     3721 accgatcata ttaagcgtgg cattgccgcc tgaccttgtg cgatttgaaa catcttcgga
     3781 agacgcgagc cagtgatttc actggctttg ttgcattttg cataacaaat gcgaggacgt
     3841 ttccagatac cacaatccat acggtatgaa catttcctcg cctcaatgtt gtagtgtaga
     3901 atgcggcgtt tctattaata cagacgttaa gctcagaaca gcgacttcta aaagcctggt
     3961 taaccgggca ttaaggcaca ataatcatac tttatgaaga atatacgtaa cttttcgatc
     4021 atagctcaca ttgaccacgg taaatcgacg ctgtctgacc gtattatcca gatctgcggt
     4081 ggcctgtctg accgtgaaat ggaggcgcag gttctcgatt ccatggatct tgagcgtgag
     4141 cgtggcatta ccatcaaagc gcaaagcgtg acgctggact acaaagcgtc tgacggcgaa
     4201 acctatcagc ttaactttat cgacaccccg ggccacgtag acttctccta tgaagtttcc
     4261 cgttcgctgg ctgcctgtga aggtgcattg ctggtggtcg acgccgggca gggcgtggaa
     4321 gcgcaaaccc tggcaaactg ctacaccgcc atggaaatgg atctcgaagt ggtgccagta
     4381 ctgaacaaga ttgacctgcc ggcagccgat cctgaacgcg tggcggaaga aattgaagat
     4441 atcgtcggca tcgacgccac tgacgcggtg cgctgttcag cgaaaaccgg cgttggcgtg
     4501 caggacgttc tcgaacgtct ggtgcgcgac attccgccgc cggaaggcga tccggaaggc
     4561 tcgttgcagg cactaattat cgactcatgg ttcgacaact acctgggcgt tgtttcactt
     4621 atccgtatta aaaacggcac cctgcgtaag ggcgacaaag tgaaagtcat gagtaccggg
     4681 cagacctata acgccgaccg tctgggcatc ttcacgccga aacaggttga ccgcactgaa
     4741 ctgaaatgtg gcgaagtagg ctggctggta tgtgcgatta aagatatcca tggcgctcca
     4801 gtcggcgata ccttaacgct ggcgcgtaat ccggcagaaa aggcgctgcc tggctttaag
     4861 aaagtcaaac cgcaggtata cgccggtctg ttcccggtaa gttccgacga ctatgaagcc
     4921 ttccgtgacg cgctgggtaa actcagcctg aatgatgcct cactgttcta tgagccggaa
     4981 agctccagcg cgctgggctt tggtttccgc tgcggcttcc ttggcctgct gcacatggag
     5041 atcatccagg aacgtctgga acgtgaatac gatctggatc tgatcaccac tgcgccgacc
     5101 gtagtgtatg aagttgaaac cacgtcaagg gaagttatct acgtcgacag cccatccaag
     5161 ctgcctgcgg taaataacat ttacgaactg cgcgagccga ttgcagagtg tcacatgctg
     5221 ctgccgcagg catatctcgg caacgttatt acgctgtgcg tagaaaaacg cggcgtgcag
     5281 accaatatgg tttaccacgg taatcaggtg gcgctgacgt acgagatccc gatggcggaa
     5341 gtggtgctcg acttcttcga tcgcctgaaa tctacctcgc gtggttatgc gtctctggat
     5401 tacaacttca aacgcttcca ggcgtccgac atggtacgtg tagacgtatt aatcaacggt
     5461 gaacgtgttg atgcgttggc gctgatcacc caccgtgata attcgcaaaa ccgcggtcgc
     5521 gagttggtgg agaagatgaa agatctgatc ccacgccagc agtttgatat cgccattcag
     5581 gcagcgattg gtacgcacat tattgcgcga tccaccgtga aacagctgcg taaaaacgta
     5641 ctggctaaat gttatggcgg cgatatcagc cgtaagaaaa agctgctgca gaagcagaaa
     5701 gaaggtaaga aacgcatgaa gcagatcggt aacgtcgagc tgccgcagga agcgttcctc
     5761 gccattctgc acgtcggcaa agacaacaaa taacccttag gagttggcat ggcgaatatg
     5821 tttgccctga ttctggtgat tgccacactg gtgacgggca ttttatggtg cgtggataaa
     5881 ttctttttcg cacctaaacg gcgggaacgt caggcagcgg cgcaggcggc tgccggtgac
     5941 tcactggata aagcaacgtt gaaaaaggtt gcaccgaagc ttggctggct ggaaaccgga
     6001 gcttctgttt ttccggtgct ggctatcgta ttgattgtac gttcgtttat ttatgaaccg
     6061 ttccagatcc cgtcaggttc gatgatgccg actctgttaa tcggtgattt tattctggta
     6121 gagaagtttg cttatggcat taaagatcct atctaccaga aaacgctgat cgaaaccggt
     6181 catccgaaac gcggcgatat cgtggtcttt aaatatccgg aagatccaaa gcttgattac
     6241 atcaagcgcg cggtgggttt accgggcgat aaagtcactt acgatccggt ctcaaaagag
     6301 ctgacgattc aaccgggatg cagttccggc caggcgtgtg aaaacgcgct gccggtcacc
     6361 tactcaaacg tggaaccgag cgatttcgtt cagaccttct cacgccgtaa tggtggggaa
     6421 gcgaccagcg gattctttga agtgccgaaa aacgaaacca aagaaaatgg aattcgtctt
     6481 tccgagcgta aagagacact gggtgatgtg acgcaccgaa ttctgacagt gccgattgcg
     6541 caggaccagg tggggatgta ttaccagcag ccagggcaac aactggcaac ctggattgtt
     6601 ccgccgggac aatacttcat gatgggcgac aaccgcgaca acagcgcgga cagccgttac
     6661 tggggctttg tgcctgaagc gaatctggtc ggtcgggcaa cggctatctg gatgagtttc
     6721 gataagcaag aaggcgaatg gccgacaggt gtgcgcttaa gtcgcattgg cggcatccat
     6781 taatagccca tcttcgttca cgttgtcgcc gttatggcga caacgtgaat tatttatgag
     6841 ataaatctcc cgtggctaac gacatccccc gtcgttgtgt atagaatatt cccccgaagt
     6901 ttaaggttgg cacctccagg ttgccacggc acacgaaaca gcgttggtcc ccatataccg
     6961 gtaaactgaa actgcagcga agcagttagc agagccatgt atatcaggtc tgtttcgtgt
     7021 gctgaattgt tgacgcattt atttattggt atcgcatgaa ccccatcgta attaatcggc
     7081 ttcaacggaa gctgggctac acttttaatc atcaggaact gttgcagcag gcattaactc
     7141 atcgtagtgc cagcagcaaa cataacgagc gtttagaatt tttaggcgac tctattctga
     7201 gctacgttat cgccaatgcg ctttatcacc gtttccctcg tgtggatgaa ggcgatatga
     7261 gccggatgcg cgccacgctg gtccgtggca atacgctggc ggaactggcg cgcgaatttg
     7321 agttaggcga gtgcttacgt ttagggccag gtgaacttaa aagcggtgga tttcgtcgtg
     7381 agtcaattct cgccgatacc gtcgaagcat taattggtgg cgtattcctc gacagtgata
     7441 ttcaaaccgt cgagaaatta atcctcaact ggtatcaaac tcgtttggac gaaattagcc
     7501 caggcgataa acaaaaagat ccgaaaacgc gcttgcaaga atatttgcag ggtcgccatc
     7561 tgccgctgcc gacttatctg gtagtccagg tacgtggcga agcgcacgat caggaattta
     7621 ctatccactg ccaggtcagc ggcctgagtg aaccggtggt tggcacaggt tcaagccgtc
     7681 gtaaggctga gcaggctgcc gccgaacagg cgttgaaaaa actggagctg gaatgagcat
     7741 cgataaaagt tactgcggat ttattgccat cgtcggacgt ccgaacgttg gcaaatccac
     7801 attgttgaac aaactgctgg ggcagaagat ctccatcacc tcccgcaaag cgcaaacaac
     7861 tcgtcaccgc attgtgggga tccatactga aggcgcgtat caggcgatct acgttgatac
     7921 ccctggcctg catatggaag aaaaacgcgc tattaaccgc ctgatgaaca aagcggcgag
     7981 cagctctatt ggcgacgttg agctggtgat ttttgtcgtt gaaggcaccc gctggacgcc
     8041 ggacgacgaa atggtgctca acaaactgcg cgatggcaaa gcgccggtga tcctcgcggt
     8101 gaacaaagtg gataacgtgc aggaaaaagc cgatctgctg ccgcacttac agttcctggc
     8161 aagtcagatg aacttcctcg atatcgtgcc gatctctgct gaaaccgggc tgaatgtcga
     8221 cactatcgca gcaatcgtgc gtaagcatct gcctgaagcg acccatcact tcccggaaga
     8281 ttacatcacc gatcgctcac agcgttttat ggcgtctgaa atcatccgcg aaaaactgat
     8341 gcgtttcctc ggcgctgaac tgccgtactc cgtgaccgtg gagatcgaac gtttcgtctc
     8401 taacgaacgc ggtggttatg acatcaatgg tttgattctc gttgagcgtg aagggcagaa
     8461 gaagatggtt attggcaaca aaggggccaa gatcaaaacc atcgggattg aagcgcgtaa
     8521 agacatgcag gaaatgttcg aagcgcctgt ccaccttgaa ctgtgggtaa aagtgaaatc
     8581 cggttgggcc gacgacgaac gcgcactgcg cagtctcggt tacgttgacg atctttaaga
     8641 gtcactccga tggaaggctg gcagcgcgca tttgtcctgc atagtcgccc gtggagcgaa
     8701 accagcctga tgctggacgt cttcacggag gaatcgggtc gcgtgcgtct ggttgccaaa
     8761 ggcgcacgct ctaaacgctc tactctgaaa ggtgcattac agcctttcac ccctctcttg
     8821 ctacgtttcg gcgggcgtgg cgaagtcaaa acgctgcgca gtgctgaagc tgtctcgctg
     8881 gcgctgccat taagcggtat cacgctttac agcggtctgt acatcaacga acttctctcc
     8941 cgcgtactgg aatacgagac gcgcttctct gaactctttt tcgattactt gcactgcatt
     9001 cagtctcttg caggtgccac tggtacgcca gaacccgcgc tgcgccgctt tgaactggca
     9061 ctgctcgggc atctgggcta tggcgtcaat tttacccatt gcgcgggtag cggcgagccg
     9121 gtagatgaca ccatgacgta tcgttatcgc gaagaaaaag ggtttatcgc aagcgtcgtt
     9181 atcgacaata aaacgttcac cggaaggcag ttaaaagcgt taaacgcgcg ggaatttcct
     9241 gacgcagaca cactacgcgc cgcgaaacgc tttacccgca tggcgcttaa gccgtatctt
     9301 ggcggtaaac cattaaagag cagggaactg ttccggcagt ttatgcctaa gcgaacggtg
     9361 aaaacacatt atgaatgatg aggattgtca tggctgaatt actgttaggc gtcaacattg
     9421 accatattgc aacgctgcgt aacgcacgcg gtactgctta cccggatccg gtgcaggcgg
     9481 cgtttattgc cgagcaggcg ggagcggacg gcattaccgt gcatttacgt gaagatcgcc
     9541 gccacattac tgaccgcgat gtgcgcatcc tgcggcagac gctggatacc cgaatgaatc
     9601 tggagatggc agtgaccgaa gagatgctgg cgatcgccgt tgagacgaag ccacattttt
     9661 gctgcctggt accggaaaag cgtcaggaag taacaaccga aggcggcctg gacgttgcag
     9721 ggcagcgtga aaaaatgcgc gatgcctgca aacgtctgac agatgccggg attcagattt
     9781 ctctctttat cgacgccgat gaagagcaga tcaaagctgc ggcagaggtt ggcgcaccgt
     9841 ttatcgagat ccacaccggt tgctatgctg atgccaaaac tgacgccgaa caggcgcaag
     9901 agctggtgcg tatcgccaaa gccgcgacct ttgccgctag tctcggtctg aaagttaacg
     9961 ccggacacgg tctgacctat cacaacgtga aagccattgc cgccatccct gagatgcatg
    10021 aactgaatat cggtcatgcc attattggcc gtgcagtgat gaccggactg aaagatgcgg
    10081 tggcagaaat gaagcgtctg atgctggaag cgcgtggcta atggcaatat taggtttagg
    10141 cacggatatc gtagagatcg ctcgcataga agcagtgatc gcccgatccg gtgagcgcct
    10201 ggcacgccgc gtattaagcg ataacgaatg ggcaatctgg aaaacgcacc accagccggt
    10261 gcgttttctg gcgaagcgtt ttgctgtgaa agaagccgca gcaaaagcgt ttggcaccgg
    10321 gatccgcaat ggtctggcgt ttaatcaatt tgaagtattc aatgatgagc tcggcaaacc
    10381 acggctacgg ctatggggtg aggcattaaa actggcggaa aagctcggcg ttgtaaatat
    10441 gcatgtaacg ctggcagatg aacgccacta tgcttgtgcc acggtaatta ttgaaagtta
    10501 aaataatacc gttctcttta aaaagtaaaa cccccgaact ggtaaggaac gggggtttta
    10561 ctttttaact ttaattttgt gcagcatgca ccctcaacaa cagcgattat ggatcagtgt
    10621 acttatcttt tacaagcaac ttgcaatctt tagcataaaa actcgagcct ttacgaagaa
    10681 agcaatactg aacggctgca ttccttgttt aaggaaaaac gaatgactga ttgccgatac
    10741 ctgattaaac gggtcatcaa aatcatcatt gctgttttac agctgatcct tctgttctta
    10801 taacacaagg aaacgtactt aaggtgcgtc cggtgaacca atcggacgcg cctttaataa
    10861 ctataaataa gtgtcttggc agatactata taaattaact tagtgaatga ttatgctaat
    10921 gtcatcaatt aaataaatat aatggcgtta aggcttccca gtaatataat taatactcta
    10981 cttccagagt agaatattaa attttatccg cgtggtgcat cagcacaaat ttatcccaca
    11041 actgttcttc tgtctcgaca tgcgccggat ctttcacaat agtattgggg atcgggcaca
    11101 ccttctggca ggttggtgtc tcgtagtgcc ctacgcattc ggtacactta tcgctgttaa
    11161 tctcgtagat atgatctccc attgaaatcg cctcattcgg gcattcgggt tcacacatat
    11221 cacaattaat acagcgttta gtaattagta aagacatttc aatggattac cgttaaatca
    11281 ttttaaaatc agtaagttgt atcgagtttg tatgctttac tgtcattaac ttactgtatg
    11341 ttgatccagt gtatttaacc ttgataaact cagtccagca acacaaaacc gcaacacatt
    11401 gcattttgtc ccgtagaaaa gacttgtatg tgtgagcttg ttttctgcgc ctacgcagat
    11461 aaggattgag aatgccgcgc actgtaacac ataatccgga tagccccaat aatgacgatg
    11521 ttttagccgc atctgaaaaa tgggacgcct gtaaaccccc ctataccagc gcacacatga
    11581 aaatctgtgt tgctgccgcc aaaatcatcc tcgctgcttc cggcgtggct cgccgttcca
    11641 aatacgaaaa agagaactat ctccgtatcg atttcagcaa agccggtaag gttacatttt
    11701 acgccgagtt tccaaaaaag atgggcctca aaggtaaaaa gctcggcgag tggccggagc
    11761 tcgctatcca gctggcgcgc gaaaaagcac taggtatggc tgacggtgga ctgcgggcag
    11821 agtccgtaca tgcagcgctg gaaatgtacc gggatgacct caaagccaaa gtagcccggc
    11881 agaagctgag cccggacagt ttcacaacct acggggtgcg tatcgaccgg attaaagcaa
    11941 cgttcggcga gcgtgaggtg ttcagcgacg taacatacaa tcggctggtg gaagtgctgg
    12001 acgagtggat cgccactcgc tcgaacaata acgccctgga gttgtttgcc gagctccgtc
    12061 ggttctggaa gttctgcgca cctactcttt gcaacggccg caatgttgcc gccagtctgc
    12121 cagatgatta tgtttcctcc cgcgtacaga aacctacccc cacacggctt tttaccgata
    12181 ttgaatcaat cgcccgactc tggctcaatg ttgctgcctg cacctctgta caccagaaga
    12241 atgctgttcg cttcatgatc atcactggtg ttcgtccgat taatgtccat aacctgcgct
    12301 gggactacgt ttacgaggag gctggtgaaa ttgtttatcc ggaaggggtt atcggcatgc
    12361 gaggggctat gaaaacacaa aaggctttcc gcctgccgat aacgcctgag atccggcgga
    12421 ttatcgacga gcagaaagcc tggcgtgatt cagttcctga gtgcaacagg gattatgtat
    12481 ttttgcagcc acgtgatcca atgcagccat tttcaaaacg atcactggat aagctggtga
    12541 aaacatacag cccggacggg gctgtaaaag gaataaaaca tgatgggact gttaaaggga
    12601 aagacggtgc atttaatacg atgtgccgta aattccttaa gagcaatgtt attgccttga
    12661 tcgataggaa ttaaaacccc aaaaagatta aaaaaacacc acaaaacgga tgtttcttca
    12721 acaccacttt tgctcatatg aacggaaccg atgattaaac tggatggctc tgattgattc
    12781 agggtatgaa tggcggtttt ttgctccgtt tccctcaaaa tggacgcaac tttccctctg
    12841 cggctctcag ccgcaccacc gcatccgggc cagcggctca tgcatcagga cctgctctgc
    12901 cagacggtag ccccgcttca gccccgtaaa acgcatctga ctcccgcaca gcacgcactt
    12961 cagcgggtca accttcagta acctctgata catccctctc caggtgattt gcatcgccgt
    13021 ttttctcact gtctccgtta tgatgtacac cacttcttcc agtaaccgcc gtttcgccgg
    13081 actcaaaaaa ccgtagtacc tcaccatacg gaacccctta tccgccacat gccaggagaa
    13141 cctttccatg aactcatctc cactcatcaa caggtattct tcccgttttg ttcggtgact
    13201 gttgtaacgc agaccgattt catcctgacc ggcataatgc tccagacgac tcatcggcac
    13261 cggtggcttt ttcaggtaag agccaaagta caccgccaca tggggggcat tatccatcac
    13321 ccgggatacg ttgacattcc agccacggcg gtaatgcgtg tccaggaagc gattccattc
    13381 ccgtttactg cttccttctg ctgccagcgc atccggcatc accaggtcag ggtatttccg
    13441 tgacagcaac cgtgttatcc ggtagcgcca catgctcatc accttacggg cgtaaaaatg
    13501 aagatttttc caggtgtggc ccgacgtcac accaccggca gttgtcgata aatggatatg
    13561 cggatgccac tgctggtcac gcccccatgt gtggatcacc gtgaatatcc ccggctccac
    13621 atctgcctga tggcagattt ccagtatcac atccgctgca atgcggctca tctctgtcag
    13681 taaccaccgg ttgtggaaca ccagggacca gtactggcag ggaagtgtga acacaatatg
    13741 ctgccacggg cagtcgggga ccaggctcag cagatactgt atccactgtg cgccagcctt
    13801 caccccgcag tgcgggcagg agcggctttt acaccggaag cagacctttt ttgtatggca
    13861 acagtccggt gatgaacagc accactgtgt ataccccatc agtgtggtcc cgcacgccat
    13921 gattttggtc accgactcaa tcaccaccgg acgtactgcc ctttccggct gcttctccag
    13981 ccagttaagc cagtggtttc cctgctgaaa gatatcggca aaacggggaa gcatcagaag
    14041 ggcggggcga ctccgtccgg ccagtgaacc gtgccacact ccgggcagta cataccgccg
    14101 gcgctgatac cggaaagaat ggtcgcaaat tcccgctccg tgcagcgggc gatttccgga
    14161 tacccttcgt catcaacacg tacaaaccag aagaccagct ttttgtttcc cgcatccaca
    14221 aagaacggaa tattcaggtc tgcgcagcat tcaacggcat cgtcaaaact atcaaagcgc
    14281 agaacttctg cgtcttcttc gtcaaaaaaa tcatcttcgt gaagcttcac gacatagcgg
    14341 ggaagtttgc ttctttgaga ggcgggttta c
//
LOCUS       DADMKF010000102        14306 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.104, whole genome shotgun sequence.
ACCESSION   DADMKF010000102 DADMKF010000000
VERSION     DADMKF010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14306
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.104"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            19..1245
                     /locus_tag="JNC24_002819"
     CDS             19..1245
                     /locus_tag="JNC24_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HAY9159521.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPFNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESEQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQDQLETLPSRD
                     LAYFMEGTEYFDDYLRAVAWAQLFASLNRDAMMENVVTALQSITQKTVRQPQTLAMEE
                     INCHHNYVQKEQHFGEEIYVTRKGAVFARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
     gene            1249..2265
                     /gene="rtcA"
                     /locus_tag="JNC24_002820"
     CDS             1249..2265
                     /gene="rtcA"
                     /locus_tag="JNC24_002820"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HAY9159522.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITSIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRLGTVRGGEYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFNTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGSFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAVVGEYLADQLVLPMALAGAGEFTVAHPSCHLLTNIAVVERFLPVRFGLIETDGVTR
                     VSIE"
     gene            complement(2308..5013)
                     /gene="malT"
                     /locus_tag="JNC24_002821"
     CDS             complement(2308..5013)
                     /gene="malT"
                     /locus_tag="JNC24_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HAY9159523.1"
                     /translation="MLIPSKLSRPVRLDHTVVRERLLAKLSGANNFRLALITSPAGYG
                     KTTLISQWAAGKNDIGWYSLDEGDNQQERFASYLIAAVQQATNGHCAICETMAQKRQY
                     ASLTSLFAQLFIELAEWHSPLYLVIDDYHLITNPVIHESMRFFIRHQPENLTLVVLSR
                     NLPQLGIANLRVRDQLLEIGSQQLAFTHQEAKQFFDCRLSSPIEAAESSWICDDVSGW
                     ATALQLIALSARQNTHSAHKSARRLAGINASHLSDYLVDEVLDNVDLATRHFLLKSAI
                     LRSMNDALITRVTGEENGQMRLEEIERQGLFLQRMDDTGEWFCYHPLFGNFLRQRCQW
                     ELAAELPEIHRAAAESWMAQGFPSEAIHHALAAGDALMLRDILLNHAWSLFNHSELSL
                     LEESLKALPWDSLLENPQLVLLQAWLMQSQHRYGEVNTLLARAEHEIKDIREGTMHAE
                     FNALRAQVAINDGNPDEAERLAKLALEELPPGWFYSRIVATSVLGEVLHCKGELTRSL
                     ALMQQTEQMARQHDVWHYALWSLIQQSEILFAQGFLQTAWETQEKTFQLINEQHLEQL
                     PMHEFLVRIRAQLLWAWARLDEAEASARSGIEVLSSYQPQQQLQCLAMLIQCSLARGD
                     LDNARSQLNRLENLLGNGKYHSDWISNANKVRVIYWQMTGDKAAAANWLRHTAKPEFA
                     NNHFLQGQWRNIARAQILLGEFEPAEIVLEELNENARSLRLMSDLNRNLLLLNQLYWQ
                     AGRKSDAQRVLLDALKLANRTGFISHFVIEGEAMAQQLRQLIQLNTLPELEQHRAQRI
                     LREINQHHRHKFAHFDENFVERLLNHPEVPELIHTSPLTQREWQVLGLIYSGYSNEQI
                     AGELEVAATTIKTHIRNLYQKLGVAHRQAAVQHAQKLLKMMGYGV"
     gene            5624..8017
                     /gene="malP"
                     /locus_tag="JNC24_002822"
     CDS             5624..8017
                     /gene="malP"
                     /locus_tag="JNC24_002822"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HAY9159524.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVTKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLIDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENPQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            8027..10111
                     /gene="malQ"
                     /locus_tag="JNC24_002823"
     CDS             8027..10111
                     /gene="malQ"
                     /locus_tag="JNC24_002823"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HAY9159525.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWLLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVKA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMAAFRQFVADQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYQNVDSPEVRQFC
                     EEHRNDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLESMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(10156..11472)
                     /gene="gntT"
                     /locus_tag="JNC24_002824"
     CDS             complement(10156..11472)
                     /gene="gntT"
                     /locus_tag="JNC24_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HAY9159526.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIDSIKVGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKRIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(11833..12408)
                     /gene="nfuA"
                     /locus_tag="JNC24_002825"
     CDS             complement(11833..12408)
                     /gene="nfuA"
                     /locus_tag="JNC24_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HAY9159527.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            complement(12467..13150)
                     /gene="gntX"
                     /locus_tag="JNC24_002826"
     CDS             complement(12467..13150)
                     /gene="gntX"
                     /locus_tag="JNC24_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HAY9159528.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRATRTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSLLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            13188..13958
                     /gene="bioH"
                     /locus_tag="JNC24_002827"
     CDS             13188..13958
                     /gene="bioH"
                     /locus_tag="JNC24_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HAY9159529.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSQGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDDWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVPMPFLRLYGYLDGLVPRKVVPML
                     DKLWPRSKSYIFAKAAHAPFISHPVEFCHLLVALKQRV"
     gene            complement(13987..>14306)
                     /locus_tag="JNC24_002828"
     CDS             complement(13987..>14306)
                     /locus_tag="JNC24_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY9159530.1"
                     /translation="LMGLVEQMACLLSSGYANDRQIKGLFNYILQTGDAVRFNDFIDG
                     VAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLADIMRFTGVSEEELAA
                     ASQ"
BASE COUNT         3442 a         3568 c         3996 g         3300 t
ORIGIN      
        1 cgaaaagaga aaaacaaaat gaattacgaa ttactgacca ctgaaaatgc cccggtaaaa
       61 atgtggacca aaggcgtgcc ggtagaagcc gatgcgcgtc agcaacttat taatacggcg
      121 aagatgccgt ttattttcaa acatattgcg gtaatgcctg atgtacacct gggtaaaggt
      181 tccaccattg gtagcgtgat cccgaccaaa ggggcgatta ttccggcggc ggtgggcgtg
      241 gatattggct gtggaatgaa cgcgctgcgt accgcgttaa cggcggcaga cctgcctgaa
      301 aacctggcgg agctgcgtca agcgattgaa acggccgtac cacacgggcg tactaccggg
      361 cgttgtaaac gtgataaagg cgcatgggaa aacccgcctt ttaacgtcga tgcgaaatgg
      421 gctgagctgg aggccggtta tcagtggtta acgcaaaaat atccgcgttt ccttaacact
      481 aacaactata aacacctggg aacgctggga acgggtaacc actttattga aatctgcctt
      541 gatgagtcgg agcaggtgtg gattatgctg cactccggtt cacgcggaat tggtaacgcc
      601 atcgggactt actttatcga tctggcgcaa aaagagatgc aggaccaact tgagacgttg
      661 ccgtcgcgtg atctggcgta ctttatggaa ggtacggaat actttgatga ttacctaaga
      721 gccgtggcct gggcgcagct tttcgccagc cttaaccgcg atgcgatgat ggaaaacgtg
      781 gtaacggcat tgcagagcat tacgcagaaa acggtcagac agccacaaac gctggcgatg
      841 gaagagatca actgtcacca caactatgtg caaaaagaac agcactttgg tgaagagatc
      901 tacgtgacgc gtaaaggcgc ggtgtttgcg cgtgctggtc aatatggaat tattccaggg
      961 tcgatgggag caaaaagctt tatcgtccgt gggctgggaa atgaagaatc tttctgttcg
     1021 tgcagccacg gtgccgggcg ggtaatgagc cgtactaaag cgaaaaaact gttcagcgtg
     1081 gaagatcaaa ttcgtgccac cgcgcatgtg gaatgccgta aagatgccga agtgatcgac
     1141 gaaatcccga tggcgtataa agatattgat gcggtgatgg cggcacaaag cgatctggtg
     1201 gaagttatct ataccctgcg tcaggtggtg tgcgtaaaag gataaatgat gaaaaggatg
     1261 attgcgctgg atggcgcaca gggcgaaggt ggcgggcaga tcctgcgctc ggcgctgagc
     1321 ctgtcgatga taaccggcca gccatttacc atcaccagca ttcgtgccgg gcgggcgaaa
     1381 ccggggctgt tgcgccagca tctgaccgcg gtaaaagcgg ctgcggaaat ttgtagggca
     1441 acggtggaag gcgcggagct ggggtcgcag cgtctggtct tccggcttgg caccgtgcgc
     1501 ggcggcgagt accgctttgc tatcggtagt gccggaagct gtacgctggt gctgcaaacg
     1561 gtgctgcccg cgctgtggtt tgccgatgga ccttcgcgtg ttgaagtgag cggaggcacc
     1621 gataacccgt cggccccgcc tgcggatttt atccgccggg tgctggagcc gctgctggcg
     1681 aaaataggaa ttcatcagca aaccacgctg ttacgtcacg gtttttatcc tgccggaggc
     1741 ggcgtggtgg caacggaagt ctcgcccgtg gcatcgttta acaccttgca acttggcgag
     1801 cgcgggaaca ttgtgcagat gcgtggagaa gttctattag ctggtgtgcc gcgccatgtt
     1861 gctgagcgtg aaatcgctac actggcgggg agtttttccc tgcatgaaca gaatattcat
     1921 aacctgccgc gcgaccaggg gccgggtaat accgtctcgc tggaagtcga aagtgaaaat
     1981 atcactgaac gctttttcgt cgtcggtgaa aagcgcgtca gtgccgaggt ggtagccgcg
     2041 cagttagtga aagaggtgaa acgctacctg gcaagcccgg cggtagtggg ggaatatctc
     2101 gccgaccagc tggtgctacc gatggcgctg gcgggcgcgg gagaatttac ggtcgcccat
     2161 ccttcatgcc atctgctgac caatatcgcc gtggtggagc gtttcttgcc ggtgcggttt
     2221 ggtttgatag aaacagatgg cgtaacgcgg gtgagtattg aatgatgtaa agatgtaaac
     2281 cggatccaat gcattatccg gctaaactca caccccatat cccatcatct tcagcagttt
     2341 ctgcgcgtgt tgtaccgcgg cctgacggtg ggcgacgccg agtttttggt acagattgcg
     2401 gatatgcgtc ttgatggtgg ttgccgccac ttccagctcg cccgcaattt gctcattgct
     2461 gtaaccggag tagatcagcc ccaggacctg ccattcacgt tgggtcaacg ggctggtgtg
     2521 gatcagttct ggcacttccg gatgattcag cagacgttca acgaaattct catcgaaatg
     2581 ggcgaattta tgccgatgat gttgattgat ttctcgcaga atacgctgcg cgcgatgctg
     2641 ttccagttcc ggcagcgtat tgagttgaat cagctggcgc agttgttgcg ccatcgcttc
     2701 gccttcgatg acaaaatgac tgataaatcc ggtgcgattc gccagtttta atgcgtccag
     2761 caacacgcgc tgggcgtcac ttttacgtcc ggcctgccag tacagttgat taagcagcaa
     2821 caggttacgg ttgagatcgc tcatcaaccg cagactgcgg gcattttcat tgagttcttc
     2881 gagaacaatt tccgcaggtt caaactcgcc cagcaagatt tgtgcgcggg caatgttgcg
     2941 ccattgacct tgcaggaagt ggttgttcgc aaactccggt ttagccgtat gacgcaacca
     3001 gttggcagcg gcggctttat cgccggtcat ttgccagtaa atcacccgga ctttgttggc
     3061 gttagagatc cagtcgctgt gatatttgcc attccccagc aggttttcca gacggttcag
     3121 ctggctacgg gcgttatcta aatcaccacg ggccagcgag cattgaatca acattgccag
     3181 gcactgaagc tgttgctgtg gctgataaga cgacaagact tcaatcccgc tacgcgccga
     3241 cgcttcggct tcatccagcc gtgcccaggc ccataacagt tgtgctcgga tgcgcaccag
     3301 aaactcatgc atcggcagct gttccagatg ctgctcgttg atcagctgga acgttttttc
     3361 ctgtgtttcc cacgcggttt gcaggaaccc ttgggcaaac agaatttcac tttgctggat
     3421 taaactccat aaggcgtagt gccagacgtc gtgctggcgt gccatctgtt cggtttgctg
     3481 cattagcgcc agtgagcggg tcaattcgcc tttgcagtgc agcacttcac ccagcaccga
     3541 ggttgctaca atgcggctat agaaccagcc cggtggcagc tcttccagtg ccagttttgc
     3601 cagccgttcc gcttcatccg gattaccatc gttaatcgcc acctgggcgc gcagagcgtt
     3661 aaattctgcg tgcatggtgc cttctctgat gtccttgatt tcatgttcag cacgggctag
     3721 cagggtgtta acttcgccat agcgatgttg gctttgcatt agccacgcct gcaataacac
     3781 taactgcgga ttttccagca aactgtccca cggcagggcc ttaagcgact cttccagcag
     3841 cgacagttcg ctatggttga acaaactcca ggcgtgatta agcagaatat cgcgcagcat
     3901 cagcgcatcg cctgccgcca gcgcatgatg aatcgcttcg ctgggaaatc cctgggccat
     3961 ccagctttct gcggcggcac ggtggatttc cggcagctcc gccgccagtt cccactggca
     4021 gcgctggcgc aggaagttgc caaacagcgg gtgatagcag aaccactcgc cggtatcatc
     4081 catccgctgt aaaaacagcc cctgacgctc aatctcttcg agacgcattt gcccgttttc
     4141 ttcgccagtc acacgggtga tgagggcatc gttcattgag cgcaaaatgg cgcttttcaa
     4201 cagaaaatgg cgcgttgcga gatcgacgtt atccaaaacc tcatcgacca gataatccga
     4261 aagatggctg gcattgattc ccgccaggcg gcgtgccgac ttatgggctg agtgggtatt
     4321 ctgccgggcg gagagggcga ttagctgtag tgccgtcgcc caaccggaaa cgtcatcgca
     4381 aatccaactg ctttctgcgg cttcaatcgg cgatgacaga cggcaatcaa aaaactgctt
     4441 cgcttcctga tgggtaaatg ccagttgctg actgccaatt tccagcagtt ggtcacgaac
     4501 acgcagattg gcaatgccca gttgcggaag gttgcgtgac aacaccacca gggtgagatt
     4561 ttctggttga tggcgaataa agaagcgcat tgactcgtgg atcactggat tagtgatcag
     4621 atgatagtca tcgatgacca gataaagtgg gctatgccat tccgccagct caatgaaaag
     4681 ctgggcgaag agtgacgtca ggctggcata ttgccgtttt tgcgccatcg tctcacatat
     4741 cgcacagtga ccgttggttg cctgctgcac ggcggcaatg agatagctgg cgaaacgctc
     4801 ttgctggtta tcaccttcat ccagcgagta ccagccgata tcgtttttgc ctgccgccca
     4861 ctgggaaatg agggtggtct ttccgtagcc cgcaggactc gtgatcagcg ccagccggaa
     4921 gttgttcgcg ccggaaagtt tagccagcag gcgctcacga accacggtat ggtcgagtcg
     4981 aaccggacga cttagttttg acggaatcag catagttaat cacttcactg tggaaaatga
     5041 ggaaatatta tttttttgcg cttcgtaatt aatggttata aggtcggcca gaaacctttc
     5101 taatgcaagc gatgacgttt ttttatgtgt ctgaatttgc actgtgtcac aattccaaat
     5161 ctttattaac aactcaccta aaacgacgct gatccagcgt gaatactggt ttcccttatg
     5221 ttcatcagat tcatttaagc aagggtttct tcttcattcc tgatgaaagt gccatctaaa
     5281 aagatgatct taataaatct attaagaatg agatggagca cattggatat tttacttgtg
     5341 aaactgtttc actcctttac ttaatttata gagttacctt ccgctttttg aaaatacgca
     5401 acggccattt tttgcactta gatacagatt ttctgcgctg tattgcattg atttgctgct
     5461 aatcctgtgg tttgcactag ctttaagtgg ttgagatcac atttccttgc tcatccccgc
     5521 aactcctccc tgcctaatcc cccgcaggat gaggaaggtc aacatcgagc ctggcaaact
     5581 agcgataacg ttgtgttgaa aatctaagaa aagtggaact cctatgtcac aacctatttt
     5641 taacgataag caatttcagg aagcgctttc acgtcagtgg cagcgttatg gcttaaattc
     5701 tgcggctgaa atgactcctc gccagtggtg gctagcagtg agtgaagcac tggccgaaat
     5761 gctgcgtgct cagccattcg ccaagccggt ggcgaatcag cgacatgtta actacatctc
     5821 aatggagttt ttgattggtc gcctgacggg caacaacctg ttgaatctcg gctggtatca
     5881 ggatgtacag gattcgttga aggcttatga catcaatctg acggacctgc tggaagaaga
     5941 gatcgacccg gcgctgggta acggtggtct gggacgtctg gcggcgtgct tcctcgactc
     6001 aatggcaact gtcggtcagt ctgcgacggg ttacggtctg aactatcaat atggtttgtt
     6061 ccgccagtct tttgtcgatg gcaaacaggt tgaagcgccg gatgactggc atcgcagtaa
     6121 ctacccgtgg ttccgccaca acgaagcact ggatgtgcag gtagggattg gcggtaaagt
     6181 gacgaaagac ggacgctggg agccggagtt taccattacc ggtcaagcgt gggatctccc
     6241 cgttgtcggc tatcgtaatg gcgtggcgca gccgctgcgt ctgtggcagg cgacgcacgc
     6301 gcatccgttt gatctgacta aatttaacga cggtgatttc ctgcgtgccg aacagcaggg
     6361 catcaatgcg gaaaaactga ccaaagttct ctatccaaac gacaaccata ctgccggtaa
     6421 aaagctgcgc ttgatgcagc agtacttcca gtgtgcctgt tcggtagcgg atattttgcg
     6481 tcgccatcat ctggcggggc gtaaactgca cgaactggcg gattacgaag ttattcagct
     6541 gaacgatacc cacccaacta tcgcgattcc agaactgctg cgcgtgctga tcgatgagca
     6601 ccagatgagc tgggatgacg cttgggccat taccagcaaa actttcgctt acaccaacca
     6661 taccctgatg ccagaagcgc tggaacgctg ggatgtgaaa ctggtgaaag gcttactgcc
     6721 gcgccatatg cagattatta acgaaattaa tactcgcttt aaaacgctgg tggagaaaac
     6781 ctggccgggc gatgaaaaag tgtgggccaa actggcggtg gtgcacgata aacaagtgca
     6841 tatggcgaac ctgtgtgtgg ttggcggttt cgcggtgaac ggtgttgcgg cgctgcactc
     6901 ggatctggtg gtgaaagatc tgttcccgga atatcaccag ctatggccga acaaattcca
     6961 taacgtcacc aacggtatta ccccacgtcg ctggatcaaa cagtgcaacc ctgcactggc
     7021 ggctctgttg gataaatcac tgcaaaaaga gtgggctaac gatctcgatc agctgatcaa
     7081 tctggaaaaa ttcgctgatg atgcgaaatt ccgtcagcaa tatcgcgaga tcaagcaggc
     7141 gaataaagtc cgtctggcgg agtttgtgaa agttcgtacc ggtattgaga tcaatccaca
     7201 ggcgattttc gatattcaga tcaaacgtct gcatgagtac aaacgccagc acctgaatct
     7261 gctgcatatt ctggcgttgt acaaagaaat tcgtgaaaac ccgcaggctg atcgcgtacc
     7321 gcgcgtcttc ctcttcggcg cgaaagcggc accgggctac tacctggcga agaatattat
     7381 ctttgcgatc aacaaagtgg ctgacgtgat caacaacgat ccactggttg gcgataagtt
     7441 gaaggtggtg ttcctgccgg attattgcgt ttcggcggcg gaaaaactga tcccggcggc
     7501 ggatatctcc gaacaaattt cgactgcagg taaagaagct tccggtaccg gcaatatgaa
     7561 actggcgctc aatggtgcgc ttactgtcgg tacgctggat ggtgcgaacg ttgaaatcgc
     7621 cgagaaagtc ggtgaagaaa atatctttat ttttggtcat accgtggaac aagtgaaggc
     7681 aattctggcc aaaggctacg acccggtgaa atggcggaag aaagataagg tgctggacgc
     7741 agtattgaaa gagctggaaa gcggtaaata cagcgacggc gataagcatg ccttcgacca
     7801 gatgctgcac agtatcggca aacaaggcgg cgatccgtat ctggtgatgg cggatttcgc
     7861 agcctatgtt gaggcacaaa agcaggtgga tgtgctgtac cgcgaccagg aggcctggac
     7921 tcgcgcggcg atcctcaata ccgcccgctg cggtatgttt agctcggatc gctctattcg
     7981 cgattatcag gctcgtatct ggcaggcaaa acgctaagga agctcgatgg aaagcaaacg
     8041 tctggataat gccgcgctgg cggcggggat tagccccaat tacatcaatg cccacggtaa
     8101 accgcagtcg attagcgccg aaaccaaacg gcgtttgctt gacgcgatgc atcaacgtac
     8161 cgccacgaaa gtggcggtaa cgccagtccc gaatgtcatg gtttatacca gcggcaaaaa
     8221 aatgccgatg gtggtggagg gcagcggcga atatagctgg ctgctgacca ccgaagaagg
     8281 aacacagtac aaaggccatg taacgggggg caaagcgttc aatctaccga cgaagctgcc
     8341 ggaaggttat cacacgctga cactcaccca ggacgaccag cgcgcgcatt gccgggtgat
     8401 tgtcgccccg aaacgctgtt acgaaccgca ggcgttgctg aataaacaaa agctgtgggg
     8461 tgcctgcgtt cagctttata cgctgcgatc ggaaaaaaac tggggtattg gggattttgg
     8521 cgatctcaaa gcgatgctgg tggatgtggc aaaacgtggc gggtcgttca tcggcctgaa
     8581 cccgattcat gcgctctatc cggcaaatcc ggagagtgcc agcccataca gcccgtcttc
     8641 tcgccgttgg ctgaatgtga tttatatcga cgttaaagcc gtcgaagatt tccatcttag
     8701 cgaagaggct caggcctggt ggcagttgcc gaccacgcaa cagacgctgc aacaagcgcg
     8761 ggatgccgac tgggtcgatt actccacggt taccgcccta aaaatgacag cattacgaat
     8821 ggcgtggaaa ggtttcgcgc aacgtgatga tgagcagatg gccgcgtttc gccagtttgt
     8881 tgcagatcaa ggcgacagcc tgttctggca ggcagccttt gatgcgctac atgcccagca
     8941 agtgaaagag gacgaaatgc gctggggctg gcctgcatgg ccagagatgt atcagaacgt
     9001 ggattcacca gaagtgcgtc agttctgcga agaacatcgt aatgacgtcg atttttatct
     9061 ctggttgcag tggctggctt acagccagtt tgccgcctgc tgggagataa gccagggcta
     9121 tgaaatgccg attggcttgt atcgtgatct ggcggttggc gtagcggaag gtggggcgga
     9181 aacctggtgt gaccgtgaac tatattgcct gaaagcgtcg gttggcgcgc cgccggatat
     9241 cctcggcccg ttggggcaga actggggatt accgccaatg gacccgcata tcatcaccgc
     9301 gcgtgcctat gaaccgttta tcgagctgtt gcgtgccaat atgcaaaact gcggcgcatt
     9361 acgaattgac catgtgatgt cgatgctgcg tttgtggtgg ataccgtatg gcgagacggc
     9421 agatcagggc gcgtatgttc actatccggt ggatgatctg ctctcgattc tggcactcga
     9481 aagtaaacgt catcgctgta tggtgattgg tgaagatctc ggtaccgtac cggtagagat
     9541 tgtcggtaag ctgcgcagca gcggtgtgta ctcctacaaa gtgctctatt tcgaaaacga
     9601 ccacgagaag acgttccgtg caccgaaagc gtatccggag cagtcgatgg cggttgcggc
     9661 gacacatgac ctgcctacgc tacgcggtta ctgggagagc ggggatctaa cgctgggcaa
     9721 aaccctgggg ctgtatccgg atgaagtggt actgcgcggt ctgtatcagg atcgcgaact
     9781 ggcgaagcaa gggctgctgg atgcactgca taaatatggt tgtctgccaa aacgtgccgg
     9841 gcataaggca tcgttgatgt cgatgacgcc gacgctgaac cgtggtttgc agcgctacat
     9901 tgccgacagt aacagtgctc tgttaggact acagccggaa gactggctgg atatggccga
     9961 accggtgaat attcctggca ccagttacca gtataaaaac tggcgacgca agctttccgc
    10021 aacgcttgag tcgatgtttg ccgatgatgg cgtgaacaag ttgctgaagg atttggacag
    10081 acggcgcaga gctgcagcga agaagaagta gagtgcggtt gatgccggat gcgatgcatc
    10141 cggcagtcag tgttatcaaa tcaccatatt cagcagcaga cagcctacca gaccgcacac
    10201 cgagataatg gtttccagca tcgaccagga tttgatggtc tcgccgatag tcaggttaaa
    10261 gtactctttg aacagccaga agcccggatc gttcacatga gagaaaatca cactaccgga
    10321 acccaccgca ataaccatca gctctgggct aacacccgtc gttgcaatca gcggtgccgc
    10381 gataccaccc gcagtgattg ccgcaacggt tgcggaaccc agcgcgatac gcagtacggc
    10441 agcaatcgac caggccatca gcagcggaga aatgttggtt tcgtgcatca tggaagcaat
    10501 gtatttgtcc acgccgctgt ctaccagcac ctgcttgaac gcaccgccac caccgatgat
    10561 caacagcatc atcgcaatga ttttgatgga agaaaccagc gtgtcgttaa tctgatccat
    10621 tgaacgacca cggttcagac caaaggtgaa catcgcaatc agcacggcaa tcagcgttgc
    10681 cattaccggg tcaccgagga actccgctac cggcaggaaa gcgtgacctt tcggcaggat
    10741 catttcggca atcgcacgca tcgccatcag cacaaccggc accagagaag tccagacgct
    10801 gacgccaaag ctcggcatct cttcttcgct gaaggttttc gcgctgtaga gaccttccgg
    10861 gattggctta tcgatacctt tcaacacgcg agcgtaaacc ggaccggcga gaatcacggt
    10921 cgggattgcc agaatagtac cgtacagcag ggttttaccc atatcggcat tgaaaatggt
    10981 ggcaatcgca gtcgggcccg gatgcggtgg caggaagccg tgggtcacag acagtgcagc
    11041 cgccattggt acaccaacat acagcagtgg aatattcgcg gaagccgcga tggtaaacac
    11101 cagcggcagc atcagcacga agcccacttc atagaacagg gcaaaaccaa cggtaaaacc
    11161 ggttaacacc accgcccact ggatgcgctt tttaccaaat ttggcaatca gcgtggtggc
    11221 gatacgttgt gcgccaccgc agtctgccag cattttgccc agcattgcgc caaaacccat
    11281 gatcagggca aggctaccga gcgtcccgcc gacaccgact ttgatggagt caataacttt
    11341 atccagcggc attccttgca ttaatccaac agcaagcgcc acgaggacga gagcgatgaa
    11401 gccgttcatt ttgaagcgga tcatcaggag caacaacaag attacaccga tagcaacaat
    11461 gactaatggc atgatttacc tggcctttca tttgttatgg gtaacgtcaa ttttctgacg
    11521 acaaactcta attatcccaa tcgggaacag agatattgcg gcaccacgac tgatacccac
    11581 taaaactaat tattgtagtc agatgtcagg agtatgtttg gtacccatgt gaatgatacg
    11641 ggtaacattt ggcgtttgag aatcaccaga gcggggtaaa tttaaattat gagaggttgg
    11701 tcatattatc gcggggaaac gaagggaggg atttgccaaa gcaatgctgt gccaacgtct
    11761 ggcacatgtt caacgtaggc ccgaaatgac gctttagcgt cgcatcgggc aatctacaaa
    11821 agaggggata acttagtagt aggagtgttc gccgcgctgg tgttcggtga gatcgcgcac
    11881 acctttcagc tccgggaatt cgttcagcag ctgcttctcg atcccttctt tcagcgtcac
    11941 atcgaccatg gaacaaccgt tacagccgcc gccaaattgc agaatggcgt aaccgtcttc
    12001 ggtgatttcc atcagcgaaa cgcgaccacc gtgaccagca agctgtgggt tgatctgcga
    12061 ctgcagcata tactccacgc gctccatcag cggtgcatcg tctgccactt tacgcatttt
    12121 ggcgttcggg gctttcagcg ttaactgaga acccaactgg tcggtaacaa agtcgatctc
    12181 tgcatcttcc aggtatggtg cgcttaactc atcaacatac gcggtcagca ggtcaaattt
    12241 cagggcagtg tcggtggctt ccacggcatc cggcggacaa taagaaacac cacattcagc
    12301 gttaggcgtg ccagggttaa tcacaaatac gcggatttgt gtcccttctt cctgatttgc
    12361 cagcagtttg gcaaagtgcg cttgtgcagc atcggaaata cggatcatag taatggccta
    12421 atagttgact attttagttg gttataatac gcccatcatc gaggctctac aaggttcgac
    12481 aaaggcacca gacctggaca gtcgccgcac cattgcgtaa aagcaactgc gcaatctctg
    12541 cgacggtact tccggtggta acgacatcat ccacaatcac catatggcga ccttgcacgg
    12601 gcaattcaag acgaaaggca tttttcaggt tgcgcttgcg cagccgggca ctgagaaaat
    12661 gctgggtcgc agtggcccgt gtacgtgtga cggcttcgct atcccattgg cagtgcaacc
    12721 agcgtgataa cggctgacac agcaaatcgc tctgattaaa tccccgacgc cagtgacgcc
    12781 gctgccataa cggaacgctg atgatgcgat ccggcaattg caacccggtg gtgcgacgag
    12841 cgtgtaagac ttccaatagt aacagacgtg acagggcgct ggcgatttca ctgcgccggg
    12901 aaaatttaag ctggtggatg agcagactta acggcggcgc atagtcggca accgtgacca
    12961 gtctttgcca gggcggcggt ttttgcaggc agcgaccgca gggaagatgg gagtgtgtgg
    13021 cgggtaatcc acattgtggg cataacgttt tatctgtgcg ggtggcgcgt gaacagaccg
    13081 aacaaatccc ccaatgacct aacgccagtg gcattcggca tagccagcat aatcccggta
    13141 ctgttagcat atgttcatcc ttgtaagtca aaagagaaca atagcggatg aataacattt
    13201 ggtggcagac caaaggtcag gggaatgttc atcttgtgct gctgcacgga tggggactga
    13261 atgccgaagt gtggcgttgc attgacgagg aacttagctc gcattttacg ctgcaccttg
    13321 ttgatctgcc cggcttcggg cgcagccagg gatttggtgc gctgtcactt gctgatatgg
    13381 ccgaagccgt gctgcaacag gcacctgata aagccatttg gttaggctgg agtctgggcg
    13441 ggctggtggc aagccagatt gcgttaaccc atcccgagcg tgttcaggcg ctggtcaccg
    13501 tggcgtcgtc accttgtttt agtgctcgtg acgattggcc ggggataaaa ccggacgtgc
    13561 tggcgggatt tcagcagcaa ctcagtgatg attttcagcg tacagtggag cggttcctgg
    13621 cgttacaaac catggggact gaaacggcgc gccaggatgc gcgggcgttg aagaaaaccg
    13681 ttctggcgtt accgatgccg gaggttgacg tgcttaatgg cgggctggaa atcctgaaaa
    13741 cggttgatct ccgtcagccg ctgcaaaacg tgcccatgcc gtttttgcga ttgtatggct
    13801 atctcgatgg tctggtgccg cgcaaagtgg tgccgatgct ggataaactc tggcctcgca
    13861 gcaaatcata tatcttcgcc aaagcggccc atgcgccgtt tatttcgcat ccggtcgagt
    13921 tttgtcacct gctggtggcg ttgaagcaga gggtgtaggc aacttttgaa atggcgagac
    13981 agaaatttac tggctcgccg cagccaactc ttcttctgac accccggtaa agcgcatgat
    14041 gtccgcaaga gggactccgg attcaagcat tattttggct atatgcaggg ctttggattg
    14101 ttccccctcc tgccgcaatc tttccgcaat tgtcattaaa ctctccttgt gtttcggtga
    14161 acgttcggca acgccgtcga taaaatcgtt aaaacgtacc gcgtcgccgg tttgcagtat
    14221 gtaattaaac agccctttga tttgtctgtc attagcgtat ccactactta ataagcaggc
    14281 catttgctct accagcccca tcaggt
//
LOCUS       DADMKF010000103        14107 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.105, whole genome shotgun sequence.
ACCESSION   DADMKF010000103 DADMKF010000000
VERSION     DADMKF010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14107
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.105"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(13..1371)
                     /locus_tag="JNC24_002829"
     CDS             complement(13..1371)
                     /locus_tag="JNC24_002829"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal sensor histidine kinase"
                     /protein_id="HAY9159531.1"
                     /translation="MKRLSITVRLTLLFILLLSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHGDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIVLCSVLSPLLIRTGLREIKKLSGVTEALNYNDSREPVEVSALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQTRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLEYLSDEKEICF
                     KVECNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDTNGYLNIDVASPGTK
                     IHEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLNKHNVFRIMLPQ
                     RN"
     gene            complement(1371..2042)
                     /gene="hprR"
                     /locus_tag="JNC24_002830"
     CDS             complement(1371..2042)
                     /gene="hprR"
                     /locus_tag="JNC24_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HAY9159532.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLRMDSVSQSVSRNNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            2175..2588
                     /gene="hiuH"
                     /locus_tag="JNC24_002831"
     CDS             2175..2588
                     /gene="hiuH"
                     /locus_tag="JNC24_002831"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HAY9159533.1"
                     /translation="MLKRYLVLSVVTAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            2697..3701
                     /gene="msrP"
                     /locus_tag="JNC24_002832"
     CDS             2697..3701
                     /gene="msrP"
                     /locus_tag="JNC24_002832"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HAY9159534.1"
                     /translation="MKKNQFLKESDVTAESVFFMKRRQVLKALGISAAALSLPHAAHA
                     DLLSWFKGNDRPPAPAGKPLEFSKPTAWQNNLPLTPVDKVSGYNNFYEFGLDKADPAA
                     NAGSLKTDPWTLKISGEVAKPLTLDHDDLTRRFPLEERIYRMRCVEAWSMVVPWIGFP
                     LHKLLALAEPTSNAKYVAFETIYAPEQMPGQQDRFIGGGLKYPYVEGLRLDEAMHPLT
                     LMTVGVYGKALPPQNGAPVRLIVPWKYGFKGIKSIVSIKLTRERPPTTWNLAAPDEYG
                     FYANVNPHVDHPRWSQATERFIGSGGILDVQRQPTLLFNGYADQVASLYRGLDLRENF
                     "
     gene            3702..4337
                     /gene="msrQ"
                     /locus_tag="JNC24_002833"
     CDS             3702..4337
                     /gene="msrQ"
                     /locus_tag="JNC24_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HAY9159535.1"
                     /translation="MRLTAKQVTWLKVSLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLATLLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQAMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLRSLFKRLRKQVHNKLSV"
     gene            4594..5244
                     /gene="zinT"
                     /locus_tag="JNC24_002834"
     CDS             4594..5244
                     /gene="zinT"
                     /locus_tag="JNC24_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HAY9159536.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDANVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKIKTFAEIKDYYHKGYA
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQFLLNEMENWPTYYPYQLSSEEVVEEMMSH"
     gene            5587..6117
                     /locus_tag="JNC24_002835"
                     /pseudo
     CDS             5587..6117
                     /locus_tag="JNC24_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416483.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
     gene            complement(6687..6776)
                     /locus_tag="JNC24_002836"
     tRNA            complement(6687..6776)
                     /locus_tag="JNC24_002836"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6740..6742),aa:Ser,seq:cga)
     gene            6870..7667
                     /gene="mtfA"
                     /locus_tag="JNC24_002837"
     CDS             6870..7667
                     /gene="mtfA"
                     /locus_tag="JNC24_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HAY9159537.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTGLTEQEQSKLVTLAE
                     RFLQQKRLVPLQGFELDSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFIPLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            7768..7843
                     /locus_tag="JNC24_002838"
     tRNA            7768..7843
                     /locus_tag="JNC24_002838"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7801..7803,aa:Asn,seq:gtt)
     gene            8157..14107
                     /gene="yeeJ"
                     /locus_tag="JNC24_002839"
                     /pseudo
     CDS             8157..14107
                     /gene="yeeJ"
                     /locus_tag="JNC24_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416485.4"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter adhesin YeeJ"
BASE COUNT         3991 a         3352 c         3170 g         3594 t
ORIGIN      
        1 tgaagcccta tgttaatttc tttgcggtaa cataatccgg aacacattat gcttattgag
       61 atagtgatac gtagcacttc ccccatgtaa ttcggcaatc gctttgacta aagaaagacc
      121 aagtccctga cctacggaat gacgcgaatt atctccccgc caaaatctac ggaagagttt
      181 ttcaggctca tgaattttcg ttccaggact ggcgacatca atattaagat agccgttggt
      241 atcaagaaaa ctggttatat gaatacgcga tttttctggt gaatatctaa tggcattaac
      301 aataagattc gataacattc gttgcagtaa aattttatcc gcaaagattt gctgattgca
      361 ctcgacctta aagcaaatct ctttctcgtc tgaaaggtat tcaagataat ccaacaaatt
      421 ttcgacttcc ttattgagag aaagcgagtc cagtttcacc aaaacattgt ttttatctgc
      481 ccgggcaaga aacagtatgt tctctgttaa ccgcgaaata ttttccagct cttcaatgtt
      541 accggcaatt gttttttgat attcagcgat acttctggtt tgactgagcg taacctgatt
      601 ctgacccagt aatgcattaa tgggcgttct aagttcatga gcgagatcgt cagcaaattg
      661 acttagacgc tcaaaatctt tgactaaagc atgatgcatt ttattcaacg cctgcccaag
      721 aggttttagt tctctcggta atgcgctaac ctcaacaggc tcccggctat cgttataatt
      781 cagcgcttcc gttacaccac tcaacttttt gatctctcgt aatcccgttc tgattaacag
      841 cggacttaat actgagcaaa gtacaatggc gacaatgcaa attataatgc tattaatttt
      901 atactgttca agcatgttat gtctggctga agccaattta gccacagtaa ccgttaatga
      961 tggcgaaact tcatcaatat taattcgtaa agcatcaatc tcagtatcat taataatgct
     1021 tctgtaaatg ccagctgcgc tgattgtctc actagcaggt atgttattta acatgtcatc
     1081 actgacattt gtccggttaa caattttatt gatgccatca ccatgaatga tcaagatatc
     1141 ctgactaaca tccatcatcc ggttaaagta tacaggtaac gtatctggat ttaccccatc
     1201 aattaacaac tgcttgatct gtgctgtccg gttaatgagt gttgtatcat cgcgccattt
     1261 caactcactt gccaggccat tatagagagt ccagacaatt ccagcgccag caacagacag
     1321 cagcaatata aaaagcaagg ttaaacggac ggttatagat aatcttttca ttattttttt
     1381 accgctacga atgaatagcc catcccccgg attgtggcga ttagcttttc aggaaaagga
     1441 tcatcaactt ttgcgcggag cctgcgaatg gcgacatcca ccgtattggt atcactatca
     1501 aagttgattc cccaaatttc actcgcaata accgttctgg gtataatttc gccagctctg
     1561 gaggccagta gccaaagtaa ctgaaactcc ttgcgcgtca gtgtaatact gatattgttc
     1621 ctgctcacac tttgacttac agagtccatt cttaagccac tgatttctaa tgttgaattc
     1681 aaagcgtgat gttgccttaa ttgtgcccga acccttgcca gcaactcaga aaatgaaaaa
     1741 ggttttacca gataatcatt tgctccactg tccagccctc tgactctgtc atcgacagaa
     1801 tcccttgcag taaggcaaat aacaggggtt tgctttgctg ttcttaacgt ttgtaagatc
     1861 tgccagccat ccatacccgg aagcataata tccagaatga tcaatgcata atcatccttc
     1921 agcgcaagat aaagcccatc tctgccatca gaaacggcat caatgacata acccgcttcg
     1981 gaaagcccct gcgttaccca ctcctgggtc ctttgattat cttcaataag taaaatcttc
     2041 atgaaaataa tatgccataa atcatatgtt atagaaacag cctagttcat tacaaaattg
     2101 taatgctgct gtaaggttac cctggccgct ttttcgctat cctcaaaact cattcacatg
     2161 acaaggatat aaacatgtta aagcgttatt tagtactctc cgtagtaacg gcagcatttt
     2221 cattaccttc tttggtttat gccgcacaac aaaacattct tagcgtgcac attttgaacc
     2281 agcaaaccgg aaaacccgct gccgacgtga cagtcactct tgaaaagaaa gcggacaacg
     2341 gctggttaca acttaatacc gccaaaacag ataaggatgg acgaattaag gcactgtggc
     2401 ccgagcaaac tgcaactacg ggcgattacc gtgtcgtatt taaaaccggg gactatttca
     2461 agaaacaaaa tcttgaaagt ttcttccctg agatccccgt tgaatttcat attaataaag
     2521 tgaacgagca ttatcatgtg cctttattac ttagccaata tgggtattca acctatcgtg
     2581 gcagttaatg cataaatatg taaaagcaga tctctgctac tgttagggag gacactccct
     2641 gacagattaa cagtaaacgg ctcttgctgg ctaacgacaa aaaagtgtga tggcttatga
     2701 aaaagaatca atttttaaaa gaatcagatg ttacggccga gtcggtattc tttatgaagc
     2761 gtcgacaggt gttaaaagca ctgggcatca gcgcagctgc actttctttg cctcacgctg
     2821 cgcatgccga tctgcttagc tggtttaaag ggaacgatcg cccgcccgcc cccgccggaa
     2881 aaccgctgga gttcagcaag cctaccgcct ggcaaaataa tctgccactg acgccagtag
     2941 ataaagtctc cggttataac aacttctatg aattcgggct ggataaagcc gatcccgccg
     3001 ctaatgctgg tagcctgaaa accgatccat ggacactgaa aatcagcggc gaagtggcaa
     3061 aaccattgac cctcgatcac gatgatttaa cccgtcgctt cccgctggaa gagcgtattt
     3121 atcgtatgcg ctgcgtggag gcatggtcga tggtggtgcc gtggattggt tttccgctgc
     3181 acaaattgct ggcgcttgcc gaacccacca gcaatgcgaa gtatgtcgct ttcgaaacaa
     3241 tttatgcacc ggaacaaatg ccaggccagc aggaccgctt tatcggcggc gggctgaaat
     3301 atccttatgt cgaaggattg cgtctcgacg aagcaatgca tccgctcaca ctgatgaccg
     3361 tgggtgttta tggcaaggcg ttaccgccac aaaatggcgc gccggtacga ctgattgtgc
     3421 cgtggaaata tggctttaaa gggattaaat cgatagtcag tattaagctg acccgcgagc
     3481 gtccgccaac cacctggaat ctggcagcgc ctgacgaata cggtttttac gccaacgtta
     3541 atccgcatgt tgatcacccg cgctggtcac aggctaccga acgatttatt ggttcaggcg
     3601 gcatcctcga tgttcagcgc cagccaacgc tactgtttaa tggttacgcc gaccaggtgg
     3661 catcgctgta tcgtggcctg gatttgcggg agaatttctg aatgcgtctg acagcaaaac
     3721 aggtgacatg gctgaaagtt agcctgcatc ttgccggatt gttgccgttt ctctggctgg
     3781 tctgggcgat caatcacggt ggactgggtg ccgatccggt gaaagatatt cagcatttta
     3841 ccggtcgcac tgcactgaaa tttttgctgg cgaccttgtt aatcacacct ctggcacgct
     3901 acgcaaaaca gccgttattg atacgcactc gccgcctgtt aggcttatgg tgctttgcct
     3961 gggcgacact gcatttaacc agttacgcat tgctggagct gggcgtgaat aatctggcgt
     4021 tattaggcaa ggagttaatt acccggcctt atttaacgtt aggaattatc agctgggtta
     4081 ttttgcttgc tttagcgttt acttcaaccc aggcgatgca gcgaaaactc ggcaagcact
     4141 ggcaacagtt gcataatttc gtctatcttg tcgcgatcct ggccccaata cattatctgt
     4201 ggtcagtgaa gattatttca ccgcagccgc tcatctacgc tgggctggct gtactgcttt
     4261 tagccttacg gtataagaag ttgcgttccc tttttaagcg gttacgcaag caggttcaca
     4321 acaaattatc tgtataactt catattgccg acaaagtacg cgttatccct gccggatgtg
     4381 gcgcgagcgc cctgtccggc ctgcgcagat cacatcaggt tatgtttcgt ttgtcatcaa
     4441 cctcaaatta tcaacataat tcattttatt tcaataagat agataagtac acctgagaaa
     4501 actatgctct catctcctaa gagttgttgc attttgccat atgttacaat ataacattac
     4561 acatcatata caatgactct ggaggaaact gttttggcaa ttcgtcttca caaactggct
     4621 gttgctttag gtgtctttat tgttagcgct cctgcctttt cgcatggtca tcactcacac
     4681 ggcaaaccct taacagaggt cgaacaaaaa gctgctaatg gtgtttttga tgatgccaat
     4741 gtacaaaacc gaacgctcag tgactgggat ggagtctggc aatccgttta tcctttactg
     4801 caaagtggca aacttgaccc cgtctttcag aagaaagcgg atgcagataa aattaaaaca
     4861 tttgctgaaa ttaaagatta ttatcacaaa ggttatgcaa cagatatcga gatgattggc
     4921 attgaggacg gcattgttga attccataga aataatgaaa caacatcctg taaatatgat
     4981 tacgatggat acaaaatact cacctataaa tcaggcaaga aaggcgttcg ttacttattc
     5041 gaatgtaaag atcctgaaag caaagcccct aaatatatac aatttagcga tcatataatt
     5101 gcaccacgaa aatcatctca ttttcacatt tttatgggta atgattcaca gcaattttta
     5161 ttaaatgaaa tggaaaactg gccaacgtat tatccatatc agttgagtag cgaagaagtg
     5221 gtcgaggaaa tgatgtctca ttgagtattc tcatgataac gcctcgatgc cgctttagta
     5281 agtcatcata actgccactg gtccatccac aaacgccact gaacgcaagc tagctacaga
     5341 cacgctcatc actatgacgt gtctgtatat taataagcta acccgcattg agttaaccaa
     5401 taacggattc catacacaat acggccaaca gagaaagata cctgtgctca cgccattgct
     5461 tatattggct ggttacaatg tgcactatca attttttaaa taacaaattc ttaaaaccac
     5521 atctgtataa ggaattttta aggttcgtgg gatagcttga ctgtaaaaat tacaggagct
     5581 acaaaaatga accgattctc aaaaactcaa atttatttac attggataac gctgcttttc
     5641 gttgcaataa cctatgccgc gatggaactc cgtggctggt ttcctaaagg tagtagtact
     5701 tatctgctga tgcgagaaac acattacaat gcgggtatat tcgtttgggt gttaatgttt
     5761 tcacgcctga ttataaaaca ccgttatagt gatccttcta ttgtaccacc gccacctgcc
     5821 tggcaaatga aagcggcttc gctaatgcac atcatgcttt atataacctt ccttgcactt
     5881 cctctgctgg ggattgcttt gatggcttac agtggaaaat cgtggagttt ccttggtttc
     5941 aatgtgtctc cctttgttac cccaaacagc gaaattaaag cactgataaa aaatattcac
     6001 gaaacctagg caaatatagg ttacttttta atcgcagctc acgctggcgc agcactcttt
     6061 catcactaca ttcagaaaga taatactctg ttacgaatga tgcatcgccg caaataagtc
     6121 gatcccctga caaggaaatg tgaaccatgt acatcaacgc gtcataaatg tttacgcaag
     6181 tgcacaatgc atcctttcta ctatcaacaa ggaggagcag aaactgtgta aatcgtacaa
     6241 tggtaagact tatcaaaaat acaggttaat tttggtattt aaaccttttt tacttgttag
     6301 tgtgatacaa aggctttcaa aaaagctggg aaattcaaca actcttggaa attatggact
     6361 ttgtcccgcg gctttaatct gacggccgcg ttcctttttt gactcaatta ttgacccctt
     6421 ctctacgcaa cttcagttcc caccaccaac tttgcggcag tttcatagga tgaatgtcta
     6481 aaagaataat ggtgagcgat aagaaaacga ctgaataact gcacattttc gctcgaaagc
     6541 ttcctgtcag atccatagcg aatcaagtgc tgaatgtcac agtatcgaac agaaaacagt
     6601 gacgatctaa cccttcaaga atattctaca attgttctgt ttaggaaaag caaggcggga
     6661 agtcaggaga taagtcattg ataaagtggc ggagagaggg ggatttgaac ccccggtaga
     6721 gttgccccta ctccggtttt cgagaccggt ccgttcagcc gctccggcat ctctccgttc
     6781 agatggttgc catgatgcca ggaaatttgg cattttaaca gtccctgttc gtgcaatttt
     6841 gttcaagtga cgagtttgcg agcaaaacga tgattaagtg gccctggaaa gtacaagaat
     6901 cagcacatca aactgccctt ccctggcagg aagcactatc gatccccctt ttaacgggtc
     6961 tgacggaaca ggaacaaagc aaattagtca ctcttgccga acgtttttta cagcaaaagc
     7021 ggcttgttcc tttacagggc tttgaactgg attcattaag aagctgccgg atagcacttc
     7081 tattttgcct acccgttctg gagttaggac tggaatggct ggatggtttt catgaagtct
     7141 taatttatcc tgcgccattt gtggtcgatg atgaatggga agacgatatc ggtctggtgc
     7201 ataaccaacg tattgttcag tcaggtcaga gctggcagca agggcctatc gttttgaact
     7261 ggttggatat acaagattct tttgatgcat ctggttttaa cctgattatt catgaagtcg
     7321 ctcataagct ggacacccgt aacggcgatc gcgccagcgg agttcccttt attccgttgc
     7381 gtgaggttgc tggctgggaa cacgatcttc atgctgcaat gaacaacatt caggaagaaa
     7441 tcgaattggt tggcgagaat gcggcgagca ttgatgctta tgcagccagc gatcctgctg
     7501 aatgttttgc cgtactttct gaatatttct ttagcgcccc agaacttttt gctcctcgtt
     7561 tcccttcatt gtggcaacgt ttctgccaat tttatcaaca agatcctttg cagagactgc
     7621 atcacgctaa tgatacagac tcgttttcgg cgacgaatgt tcattaatta acaactttgc
     7681 agattaatta accaattgaa atgacttatg aaatttagtg ttgacagaca aggtaccgct
     7741 aagtaatatg tgccccgttc acacgattcc tctgtagttc agtcggtaga acggcggact
     7801 gttaatccgt atgtcactgg ttcgagtcca gtcagaggag ccaaattcta aaaattcgct
     7861 tttttagcgc aatgtcactg accttagttg aacattgttt tttaacggat agcgggtttt
     7921 taacacctta agcgccctcg acctttatgg ttgagggcgt tttgctatga acgccatcac
     7981 cattttcccc tcgattataa aacttgagtt attcagtagt ctcccctctt gcaactcaca
     8041 cccaaaactg cctaacgaaa agttattaat tttcaatcgt attgctatca gtatttacat
     8101 tttttcgctg tgctagaaag ggcgcattta tgttagctcg ttcagggaag gtaagcatgg
     8161 ctacgaagaa gagaagtgga gaagaaataa atgaccgaca aatattatgc gggatgggaa
     8221 ttaaactacg ccgcttaact gcgggtatct gtctgataac tcaacttgcg ttccctatgg
     8281 ctgcggcagc acaaggtgtg gtaaacgccg caacccaaca accagttcct gcacaaattg
     8341 ccattgcaaa tgccaatacg gtgccctaca cccttggagc gctggaatcg gcccaaagcg
     8401 ttgccgaacg tttcggtatt tcggtggctg agttacgcaa actcaaccag tttcgtacgt
     8461 ttgctcgagg ttttgataat gtccgccagg gtgatgaact ggatgtcccg gcacaagtta
     8521 gtgaaaaaaa attaaccccg ccgccgggta ataacagtga caacctcgag caacagatag
     8581 ccagtacttc acagcaaatc gggtctctgc tcgccgaaga tatgaacagc gagcaagcgg
     8641 caaatatggc gcgtggatgg gcctcttctc aggcttcagg cgcaatgaca gactggttaa
     8701 gccgcttcgg taccgcaaga atcacgctgg gcgtggatga agattttagc ctgaagaact
     8761 cccagttcga ttttctccat ccgtggtatg aaacgcctga taatctcttt ttcagtcagc
     8821 atactctcca tcgtactgac gagcgtacgc agattaacaa cggcttaggt tggcgtcatt
     8881 tcactcccac atggatgtcg ggcatcaact tctttttcga ccacgatctt agccgttacc
     8941 actcccgcgt cggcattggc gcggagtact ggcgcgacta tctaaaatta agcagtaacg
     9001 gctatttgcg actgaccaac tggcgcagcg cacctgaact ggacaacgat tataaagcac
     9061 gcccggccaa tggctgggat gtacgcgcag aaagctggct acccgcctgg ccgcaccttg
     9121 gcggtaaact ggtctatgaa cagtattatg gcgattaagt ggccctgttc gataaagacg
     9181 atcggcaaag taatcctcat gccataaccg ctggacttaa ctataccccc ttcccgctga
     9241 tgaccttcag cgcggagcaa cgccagggta aacagggcga aaatgacacc cgttttgccg
     9301 tcgattttac ctggcaacct ggcagcgcaa tgcagaaaca gcttgacccg aatgaagtcg
     9361 ctgcacggcg tagccttgca ggcagccgtt atgatctggt ggatcgcaac aacaatatcg
     9421 ttctggaata tcgcaaaaaa gaactggttc gcctaaccct gacagacccc gtgacaggga
     9481 agtcaggaga agtgaaatca ctggtttcgt cgctacaaac caaatatgcc ctgaaaggct
     9541 ataacgtcga agccaccgca ctggaagctg ccggtggcaa agtggtcaca acgggtaaag
     9601 atattctagt taccctgccg gcttaccggt tcaccagtac gccagaaacc gataacacct
     9661 ggccgattga agtcaccgcc gaagatgtca aaggcaattt gtcgaatcgt gaacagagca
     9721 tggtggtcgt tcaggcacct acgctaagcc agaaagattc ctcggtatcg ttaagtaccc
     9781 aaacattgaa cgcggattcc cattcaaccg ccacactgac ttttattgcg catgatgcag
     9841 caggtaatcc tgttgtcggg ctggtgctct cgacgcgtca cgaaggtgtt caggacatca
     9901 ccctttctga ctggaaagat aatggtgacg gaagctatac ccagatcctg accacaggag
     9961 cgatgtctgg cacgctgacg ctgatgccac agctgaatgg tgtggatgcg gctaaagccc
    10021 ccgccgtggt gaatatcatt tctgtttcgt catcccgaac tcactcgtca attaagattg
    10081 ataaggaccg ttatctctcc ggcaatccta tcgaggtgac ggtagaactg agagatgaaa
    10141 atgacaaacc tgttaaggaa caaaaacagc aactgaataa cgcagtcagc atcgacaacg
    10201 tgaaaccagg agtcactaca gactggaaag aaaccgcaga tggcgtctat aaggcgacct
    10261 ataccgccta taccaaaggc agtggactta ctgcgaagct attggaatga agatttgcat
    10321 accgctggat ttatcatcga cgccaacccg cagtcagcga aaattgcgac attatctgcc
    10381 agcaataatg gtgtactcgc caatgagaat gcagcaaaca ccgtctcggt caatgtcgct
    10441 gatgaaggaa gcaacccaat caatgatcat accgtcacgt ttgcggtatt aagcggatcg
    10501 gcaacttcct tcaacaatca aaacaccgca aaaacggatg ttaatggtct ggcgactttt
    10561 gatctgaaaa gtagtaagca ggaagacaac acggttgaag tcacccttga aaatggcgtg
    10621 aaacaaacgt taatcgtcag ttttgtcggc gactcgagta ccgcgcaggt tgatctgcag
    10681 aagtcgaaaa atgaagtggt tgctgacggc aatgacagcg ccacaatgac cgcgaccgtc
    10741 cgggatgcaa aaggcaacct gctcaatgac gtcatggtca ctttcaatgt taattcagca
    10801 gaggcgaaac tgagccaaac cgaagtgaat agccacgacg ggatcgccac agctacgctg
    10861 accagtttga aaaatggtga ttatagggtt acggcctctg tgagctctgg ttcccaggct
    10921 aatcaacagg tgaattttat cggtgatcaa agtactgctg ccctgaccct cagtgtgcct
    10981 tcaggtgata tcaccgtcac caacacagct ccgcaacata tgactgcaac cttgcaggat
    11041 aaaaatggca acccactaaa agataaagaa atcaccttct ctgtgccaaa cgacgtcgca
    11101 agtaagttct cgattagcaa cggaggaaaa ggcatgacgg atagtaacgg ggttgcaatc
    11161 gcctccctga ccggcacgtt agcgggcacg catatgatca tggctcgtct ggctaacagc
    11221 aatgtcagcg atgcacagcc aatgacgttt gtggcggata aagacagagc ggttgtcgtt
    11281 ttgcaaacat cgaaagcgga aatcattggg aatggcgtgg atgagacaac tctgacagca
    11341 acagtgaaag atccgtcgaa tcatccggtg gcggggataa cggtaaactt caccatgcca
    11401 caggacgttg cggcaaactt tacccttgaa aataacggta ttgccatcac tcaggccaat
    11461 ggggaagcgc atgtcacgct gaaaggtaaa aaagcgggca cgcatacggt taccgcaacg
    11521 ctgggtaata acaataccag tgattcgcag ccggtaacat ttgtggcgga caaagcctcg
    11581 gctcaggttg tcctgcagat atcaaaagat gagatcacag gtaatggcgt cgatagcgca
    11641 acgctaactg caacggttaa agatcagttc gacaatgagg tgaataatct tccggtaaca
    11701 ttcagctcag cctcttcagg actcaccctg accccgggag taagtaatac caatgagtct
    11761 ggcatcgcgc aggccactct cgcaggcgtt gcctttggtg agcagacggt cactgcatca
    11821 ctggctaata atggtgccag cgacaacaaa actgtgcatt ttattggcga cacagcggcg
    11881 gcaaaaatta tcgagttgac gcctgtccca gacagcataa tcgccggtac cccgcagaac
    11941 agctccggca gcgccatcac cgccacagtc gttgataata atggctttcc ggtgaaaggt
    12001 gtgactgtga acttcaccag cagaacaaac tctgccgaaa tgacgaatgg cggccaagcc
    12061 gtaacgaacg aacagggtaa ggctaccgtc acttatacca atacccgctc ctcgatagaa
    12121 tcaggagcga gaccggatac cgttgaggcc agtctggaaa atggtagctc cacgcttagc
    12181 acatcaatta atgtcaacgc tgatgcgtct acggcacatc tcacctggct acaggcactt
    12241 tttgatacag tctccgcagg cgagacaacc agtctgtata ttgaggtgaa ggataattac
    12301 ggcaacggtg tcccccagca tcaggtgacc ctcagcgttt caccaagtga aggcgtgacc
    12361 cccagtaata acgctatata tactaccaac cacgacggca atttttacgc aagctttacc
    12421 gctacaaaag ccggggttta tcaagtgacg gcaaccctcg aaaatggcga ttcgatgcaa
    12481 caaacagtga cctatgtgcc gaacgtcacg aatgccgaaa tcacgctggc aacctcgaag
    12541 gatccggtga ttgccgacaa taacgatctc acgacactaa cagcaacagt cgctgataca
    12601 gagggcaatg cgatagccaa cactgaagta acatttactc tgccggaaga tgttaaggcg
    12661 aacttcacgc tgagcgatgg cggtaaagcg attactgatg ctgaaggcaa agcgaaagtc
    12721 acgctgaaag gtacaaaagc aggcgctcat actgttacag catcgataac tggcggtaag
    12781 agtgagcagt tggtggtgaa ctttactgcg gatacgctca ctgcgcaggt taatcttaac
    12841 gttactgagg acaattttat cgctaataac gtcgggatga ccaggctgca ggcaacagtg
    12901 actgatggaa acggcaaccc gttagccaat gaggcggtga cattcacgct accggcagat
    12961 gtgagcgcaa gctttactct cggacaaggc ggttccgcca ttactgacat caacggcaag
    13021 gctgaagtta cactgagcgg tacaaaatcc ggcacctacc ccgtgacagt tagcgtgaac
    13081 aattatggtg tcagtgatac gaaacaggtg actttgattg ccgatgctgg taccgcaaca
    13141 ctagcctcct taacctctgt atactcattc gtcgtcagca cgaccgaagg cgcgaccatg
    13201 actgcaagcg tcactgacgc taacggcaac ccggtagaag gcataaaagt taatttccgc
    13261 ggaacctccg tcacgctaag cagcaccagc gttgaaacgg atgatcgggg tttcactgaa
    13321 attcttgtga caagcaccga agtcggactg aaaacagttt cagcctctct ggcagataaa
    13381 cctactgaag tcatatcgcg attactgaat gcaaaagcag atattaattc tgcaacgatt
    13441 accagtctgg agatacctga aggtcaggtc atggtcgcac aagacgtagc agttaaagct
    13501 cacgtcaacg accagtttgg caacccgatt cttaatgaat ctgtaacatt cagtgcagag
    13561 ccaccagagc acatgaccat cagccaaaat attgtctcta ctgatacgca tggtatagcc
    13621 gaggtctcca tgacgcccga aagaaacggt tcgtatatgg tgaaagcatc cctggcgaat
    13681 ggagcctcac ttgagaaaca actggaggct attgatgaaa aactgacact ctcggcgtcc
    13741 agcccgctta tcggtgtcaa ttccccaaca ggtgcaactc tgacggcaac gctaacttct
    13801 gcaaatggca ctccagtgga gggtcaggtc atcaacttta gcgtaacgcc agaaggtgcg
    13861 acgttaagtg gcggaaaagt gagaactaac tcttctggtc aggctccagt cgttctgacc
    13921 agcaataaag tcggtacata tacggtgact gcatcgttcc ataacggcgt aacaatacag
    13981 acacagacaa ccgtgaaagt cactggcaac tcaagcaccg cccatgttac tagctttatc
    14041 gctgatccat cgactatcgc cgccaccaac agtgatttaa gtaccttaaa ggcaacggtt
    14101 gaggatg
//
LOCUS       DADMKF010000104        14103 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.106, whole genome shotgun sequence.
ACCESSION   DADMKF010000104 DADMKF010000000
VERSION     DADMKF010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14103
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.106"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            254..2020
                     /gene="adeD"
                     /locus_tag="JNC24_002840"
     CDS             254..2020
                     /gene="adeD"
                     /locus_tag="JNC24_002840"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HAY9159538.1"
                     /translation="MNNSINHKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLIN
                     GGEISGPIVIKGRYIAGVGAEYADAPALQRIDARGATAVPRFIDAHLHIESSMMTPVT
                     FETATLPRGLTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCD
                     VNGASFTLEQMLAWRDHPQVTGLAEMMDYPGVISGQNALLDKLDAFRHLTLDGHCPGL
                     GGKELNAYITAGIENCHESYQLEEGRRKLQLGMSLMIREGSAARNLNALAPLINEFNS
                     PQCMLCTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGL
                     LAPGKQADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESARLAQSAPPYGNTIARQP
                     VSASDFALQFTPGKRYRVIDVIHNELITHSHSSVYSENGFDRDDVCFIAVLERYGQRL
                     APACGLLGGFGLNEGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNG
                     QVQSHLPLPIAGLMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPAL
                     KLTSQGLFDVEKFAFTTLEVTE"
     gene            complement(2065..3456)
                     /gene="uhpT"
                     /locus_tag="JNC24_002841"
     CDS             complement(2065..3456)
                     /gene="uhpT"
                     /locus_tag="JNC24_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HAY9159539.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(3594..4913)
                     /gene="uhpC"
                     /locus_tag="JNC24_002842"
     CDS             complement(3594..4913)
                     /gene="uhpC"
                     /locus_tag="JNC24_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HAY9159540.1"
                     /translation="MLPFLKAPADAPLMADKYEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIINILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAAAALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGISALLLLPFLNAQTPREA"
     gene            complement(4923..6425)
                     /gene="uhpB"
                     /locus_tag="JNC24_002843"
     CDS             complement(4923..6425)
                     /gene="uhpB"
                     /locus_tag="JNC24_002843"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HAY9159541.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLVLP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHPVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            complement(6425..7015)
                     /gene="uhpA"
                     /locus_tag="JNC24_002844"
     CDS             complement(6425..7015)
                     /gene="uhpA"
                     /locus_tag="JNC24_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HAY9159542.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(7090..7380)
                     /gene="ilvN"
                     /locus_tag="JNC24_002845"
     CDS             complement(7090..7380)
                     /gene="ilvN"
                     /locus_tag="JNC24_002845"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HAY9159543.1"
                     /translation="MQNTTHDNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(7384..9072)
                     /gene="ilvB"
                     /locus_tag="JNC24_002846"
     CDS             complement(7384..9072)
                     /gene="ilvB"
                     /locus_tag="JNC24_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HAY9159544.1"
                     /translation="MASSGTTLTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPGPVWIDIPKDVQTAVFEIEAQPAVAEKAAAPAFSEESIRDVATMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPQVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPSPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            complement(9178..9276)
                     /gene="ivbL"
                     /locus_tag="JNC24_002847"
     CDS             complement(9178..9276)
                     /gene="ivbL"
                     /locus_tag="JNC24_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HAY9159545.1"
                     /translation="MTTSMFNAKLLPTAPSAAVVVVRVVVVVGNAP"
     gene            9841..9930
                     /gene="tisB"
                     /locus_tag="JNC24_002848"
     CDS             9841..9930
                     /gene="tisB"
                     /locus_tag="JNC24_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HAY9159546.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            10210..11394
                     /gene="emrD"
                     /locus_tag="JNC24_002849"
     CDS             10210..11394
                     /gene="emrD"
                     /locus_tag="JNC24_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HAY9159547.1"
                     /translation="MKRHRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(11402..11899)
                     /locus_tag="JNC24_002850"
     CDS             complement(11402..11899)
                     /locus_tag="JNC24_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HAY9159548.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PYLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRCDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(11896..12258)
                     /locus_tag="JNC24_002851"
     CDS             complement(11896..12258)
                     /locus_tag="JNC24_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HAY9159549.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(12248..12595)
                     /locus_tag="JNC24_002852"
     CDS             complement(12248..12595)
                     /locus_tag="JNC24_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HAY9159550.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            12704..13153
                     /locus_tag="JNC24_002853"
     CDS             12704..13153
                     /locus_tag="JNC24_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9159551.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTLESIV
                     ADKTAEIKRGLLAGIKGEKITTVEKKEDVDVDKILDQSGIALAIAALLCAFVGGMRKE
                     NRWGIRGALVFGGGTLAFHTLLFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            complement(13402..>14103)
                     /gene="tnpB"
                     /locus_tag="JNC24_002854"
     CDS             complement(13402..>14103)
                     /gene="tnpB"
                     /locus_tag="JNC24_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element transposase accessory
                     protein TnpB"
                     /protein_id="HAY9159552.1"
                     /translation="STPVHPSASMVGLDAGVAKLATLSDGTVFEPVNSFQKNQKKLAR
                     LQRQLSRKVKFSNNWQKQKRKIQRLHSCIANIRRDYLHKVTTTVSKNHAMIVIEDLKV
                     SNMSKSAAGTVSLPGRNVRAKSGLNRSILDQGWYEMRRQLAYKQLWRGGQVLAVPPAY
                     TSQRCVCCGHTAKENRLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQSG
                     RSLKQEPTEMIQATA"
BASE COUNT         3333 a         3899 c         3657 g         3214 t
ORIGIN      
        1 tcccatgctg aggtagttta aataatcgcg aaagcgtccc tgattcatta ctcacgtaat
       61 cggtattgtc attattcatt tttttgtcca ttggaggaga tttaatccct tgcttttgta
      121 aaacaaatga caacaacggt tcagtgataa ttatcacatt tcaattgcac attaatggat
      181 attctttaat aatctcgcga cgtttcttta tgataaataa taatcaaatt gataaaatca
      241 aaatgagaaa aatatgaata attctattaa ccataaattt catcacatta gccgggctga
      301 ataccaggaa ttgttagccg tttcccgtgg cgacgctgtt gccgattata ttattgataa
      361 tgtctctatt ctcgacctga tcaatggcgg agaaatttcc ggcccaattg tgattaaagg
      421 acgttacatt gccggtgttg gcgcagaata cgctgatgct ccggctttgc agcggattga
      481 tgctcgcggc gcaacggcgg tgccaaggtt tattgatgct cacctgcata ttgaatccag
      541 catgatgacg ccggtcactt ttgaaaccgc taccctgccg cgcggcctga cgaccgttat
      601 ttgcgaccct catgaaatcg tcaacgtgat gggagaagcc ggattcgcct ggtttgcccg
      661 ctgtgccgaa caggcaaggc aaaaccagta cttacaggtc agctcttgcg tacccgccct
      721 ggaaggctgc gatgttaacg gtgccagttt tacccttgaa cagatgctcg cctggcggga
      781 ccatccgcag gttaccggcc ttgcagaaat gatggactac cctggcgtaa ttagcgggca
      841 gaatgcgctg ctcgataaac tggatgcatt tcgccacctg acgctggacg gtcactgccc
      901 gggtttgggt ggtaaagaac ttaacgccta tattactgcg ggtattgaaa actgccacga
      961 aagttatcag ctggaagaag gacgccggaa attacaactc ggcatgtcgt tgatgatccg
     1021 cgaagggtcc gctgcccgca atctcaacgc gctggcaccg ttgatcaacg aatttaacag
     1081 cccgcaatgc atgctctgta ccgatgaccg taacccgtgg gagatcgccc atgaaggaca
     1141 catcgatgcc ttaattcgcc gcctgatcga acaacacaat gtgccgctgc atgtggcata
     1201 tcgcgtcgcc agctggtcga cggcgcgcca ctttggtctg aatcacctcg gcttactggc
     1261 acccggcaag caggccgata tcgtcctgtt gagcgatgcg cgtaaggtca cggtgcagca
     1321 ggtactggtg aaaggcgagc cgattgatgc gcaaacctta caggcggaag agtcggcgag
     1381 actggcacaa tccgctccgc catatggcaa caccattgcc cgccagccag tttccgccag
     1441 cgactttgcc ctgcaattta cgcccggaaa acgctatcgg gtcattgacg tcatccataa
     1501 cgaattgatt acgcactccc actccagcgt ctacagcgaa aatggttttg atcgcgatga
     1561 tgtgtgcttt attgccgtac ttgagcgtta cgggcaacgg ctggctccgg cttgtggttt
     1621 gcttggcggc tttggactga atgaaggtgc gctggctgcg acggtcagcc atgacagcca
     1681 taatattgtg gtgatcggtc gcagtgccga agagatggcg ctggcggtca atcaggtgat
     1741 tcaggatggc ggcgggctgt gcgtggtacg taacggccag gtccaaagtc atctgccgtt
     1801 gcccattgcc ggcctgatga gcaccgacac ggcgcagtcg ctggcggaac aaattgacgc
     1861 cttgaaagcc gccgcccgtg aatgcggtcc gttacccgat gagccgttta ttcagatggc
     1921 gtttctttct ctgccagtga tccccgcgct aaaactaacc agtcaggggc tatttgatgt
     1981 cgagaagttt gccttcacta cgctggaagt cacggaataa taaaaaagcc cggcgtcatg
     2041 ccgggcaaaa gtcaccagtt acgtttatgc cactgtcaac tgctgaattt ttttctcgcg
     2101 gcggattttg cgttcttcca tcaccgccac tatcgccatc aggcagatac aaccaatcgc
     2161 ggcgatatca agtgcggcga aggtgcctgc ccagccggta aggccgaata ccggcgtccc
     2221 atcggcaatc attcccagac ctaacttggc aaagctgtca ccaatcaggt aagcaaaggt
     2281 gcctttaata ccatcggcag cgccaatcgc ttttttaggt acaaagccaa cagcagccac
     2341 accaatcaac aattgcgggc caaagaccag gaaacccaac gcaaagagag aagccagata
     2401 gatatattcg ttactggcat gttgatacac accgagcgtg gcgataatca gcgccagcgc
     2461 gatgcaggcc accaggccac ggcgaccgtt cgccaggtca gagagccagc cccacagcag
     2521 cgtaccgacc agcgcaccag cttcaaacag cgtaaagccc tgaatcgcca ccgctttaga
     2581 gagtttcagt tcctggaacg cgtatacggt tgaccactgg tcgataccaa tacgtaccac
     2641 atagaggaaa atgttggcga agcacagcag ccagatcact ttgtttttca gcacatactc
     2701 aacaaagatc tgccacttgg tcatatcggt agattctgtc tctttgtcct cttcgctgat
     2761 ctcctcgccg aacagttctt cagctttgcc gaggccataa gattccgggg agtcgctgcc
     2821 gtaacgcagg ccgataaaac cgacaatcag cgcgataatc gacgggaaga taaacatgcc
     2881 gatgacatgg ccatcgaaca ggtaatttgc cccgaacagc gccacgcctg ctgcgcctgc
     2941 accgccaagg ttgtgagaaa tattccagaa accgaggaat gtcccgcgtt tacgacgcgg
     3001 cgtccatttg gtgatggtgg agtaactgca cgaaccgccg gtactctgga aaaagccgct
     3061 taaggcgtag aaggcaatca tcaggaacag gctaaccgag ccgctgccca tactggcact
     3121 gaagcccagc atacaaatgg cagacaggat cagcatgaac ggcaggaatt gtttggtgtt
     3181 tttgccgtcg gcgtagtagg aaaccagcgt tttacccacg ccataagtga tggagaaacc
     3241 caggccgatc atccccagct gcgtcatgct caacccgtag gtcgagatca tgtcgttctg
     3301 cgcgatgtta aagttcttgc gaatcaggta catcgtcagg tagccgataa agaccaccag
     3361 gtaggattgc atgaacggtt tgaaccacat tttgcgccgc acttcgagcg gaaggtccag
     3421 ggtcggcttg cgaacctggt ttaagaaagc cagcatgggt tactcctgaa atgaatacct
     3481 gcgtgaggca tgcattgtaa aaatcagcag caaatcaggc ctgagatagc gtccaggcaa
     3541 aacctggaaa atttcttagt tttgccggat atgaagtgaa aaggtgagat gcatcacgct
     3601 tcgcgcggtg tctgggcgtt caaaaagggc agtaacagca gtgcggaaat cccggcggcg
     3661 atagagataa ccacaaaaaa tccgctccag tgccaggtat cgagtacttt cgccagcggc
     3721 caaccagcaa gcgacgcccc cagataagca aacaagccga caaaccccgt cgccgcccct
     3781 gccgcctctt tgtgggaaca ctctgccgcc gccataccga ttaacatctg tgggccaaag
     3841 acaaaaaaac caatggtgaa gaagcaggtt gcctgcatca cgtagctggc aaatggcatc
     3901 agccacaggg agccgactga aagcaaaatt ccggcggcga aaatcaaatt catcggccct
     3961 cggttgccgt taaacaattt gtccgagccc caacccgcta ccagcgcacc gataaatcca
     4021 cccagttcaa acatcgtcac tgccgtattc gccgtgacca gatcgacgcc cagcgtctcg
     4081 gacatataca aattgcccca gtcgttgatc gccgcccgga ccacatagac cagcacatag
     4141 caaaacgaaa gcagccagat atacggattc agcaacacat atttggtgag gatctcttta
     4201 cgcgtcagcc ctgccccttc ttgttgttga gctatctcca gcgcgtcgtg tcgccattca
     4261 ccgaccgccg gtaaacctaa cgcctgcggg cgatcgcgca gccgccagca gagaaaaatt
     4321 cccacgacga tcgccataca accagcaatc atcatcccgg cacgccagcc gtaatgtagt
     4381 gcagccgctg ccatcacaat gggaatgagt gcgccgccga cgttatgcgc cgtgttccat
     4441 aatgcccacc aaccaccgcg ctcggtacgt gaataccagg ccgttaacag acgcgcacac
     4501 accggtgaac cccagccctg gaaaaaggcg ttcagcaccc agagtacggc aaacgcccat
     4561 agcgacgtcg agaagccaaa cagaatgttg ataatgcccg tggcgataag ccctatcccc
     4621 ataaaataac gggcatttga gcgatcgctg acaatgccgg agacaaactt cgacacgcca
     4681 taggtaatgt aaaacagggt cgctaacagg ccgatatcgc tacggctgag cacgccgtta
     4741 gcaaggattt ctggtacggc ggcgttaaaa cttttccgcg tgaagtaaaa cagcgcgtaa
     4801 cccagccaga tggtcagcag aatatgccga cgccagtagc gatagcgggc atcaatttca
     4861 tatttatcag ccattaatgg cgcatcggca ggcgctttca gaaacggcaa catcatcaaa
     4921 ccttagacat agcgttgagg tagagaaacg ctgacacgcg tgccgtgcag acaggaaatg
     4981 tgtaatgtgc cacccagcgc cgttacgcgc tcgcgcattc cggtgaggcc aaaaccttgt
     5041 tgcccggaac ccggcggcaa accgctgcca tcgtcttcaa taaccagcat caaccgttca
     5101 tcctgctgcc agccttgcag ggtgaccgcg ctggcatcag catgtttcac aatgttgttc
     5161 agcccttcct ggcagacacg aaacagcgtc acgcgctggt tttcgcttaa cgctgattca
     5221 tcgattcgcc attcgagatg gctgacaata ccgcgccctt ccagctccat ttcccgcatc
     5281 agtgagcgga tggcctgctc cagggtgaga tcatccaact ggcgcggacg taaccgaccc
     5341 aacaaacggc gcaccgcgtc gtaaacgccc agcgatagtt gttcgatgag ttgcccgctc
     5401 tgcttcacgc tggcgttatc tgccgccagc cgctgaacaa tgcccgcctg agtacgaata
     5461 gcagtgatgg tctggccgat atcatcatgc agctcacgcg ccacatcacg gcgcacgctc
     5521 tcttcggttt ccagcaaccg ttcagccaga tgctgattgc gcgccagttc cttttgcagc
     5581 gactggttaa gttcacgcaa ccgctggatg ccagcgccaa gcaacaaccc tgtcagactt
     5641 tgcaccagca gcgagagcaa taaatccacc ggatgatcgc gccaggtttg actggcgatc
     5701 agcgcgatgg cgttcatcaa cgtcgcaatc agcgcccctt gccaaccata gtgccaggcc
     5761 agcgcgataa tcggcagcac cagacagaat ggcgtaaagc gcgacagttc gtccggcaat
     5821 cccaactgga gccagagact gataacaaac agcagcaagt accagaccag atgtcgcccg
     5881 cgccagttga ttggctgaga aaccagtgac gggccgagcg gcagccaggt gttattggcg
     5941 agatagtgcc agaacaccag acatatcggg gccagcgtca ggccgccagt taaagtcagc
     6001 aacagcgcat tccacgactc tttgccgtgc caaagccagg gcagcgactg caacaacgcc
     6061 gccgccgtta acgccgcccc ctgtaacagc aaggtgcgcc agtcacgctg atggcgatag
     6121 cgcgagatca gcgctacggg cagtaacgtc agtaaactac cgatcatcaa taacggaaaa
     6181 tgggttaaac cgaccgcctg cgttagccag taaatcagca gccactccgc acccagcaat
     6241 acgggccagt atccgcgcgg gcattgcagc attagcccca gacgcagacc aaacggaaat
     6301 aacagcaccg ccatatcagg gcgctcaacc agatgcaggc tgatactcca caggcaaaac
     6361 catgcggcag agaagataaa aaagcaggca ataacggtaa ttaagcggga gaacaacgtc
     6421 ttcatcacca gccatcaaac atgcggcgcg ccagctctac gtcgttactg acgcccagtt
     6481 tttccatcag attggcgcga tggacgtgta ccgttttcgg tgacaagccc agttcggcgg
     6541 caatctcttt caccgccatt ccttgcgcca gtttttccgc cacctggcgt tcacgtttgg
     6601 ttagcgggtc ctgacgaccg gatgccagtt taatggcaat atccggcgtc agataacagc
     6661 cacccgtggc aaccgtatgc accgcagcaa tgagttcatc cgggctacag cgtttggaaa
     6721 gaaagccgcg tgcccccgcg ttaagcgcct gctcaaccag cgcaggactg tcgtgaacgg
     6781 agagcataat cgtcgccata cctttcggca gctggcttag cagctccaga ccggagatat
     6841 cgggcatgga gatatcgcaa atacacacct gcacaccgcg ccccggcagc cccgccagcg
     6901 cctcgcgccc cgaaccaaac tcggcaacta cctgcaaatc aggttccagc cccagcagct
     6961 gcgcaaagcc ggagcggacg atgaggtgat cgtctataag ggcaacggtg atcatggtct
     7021 tgtcctggcg ggtaaaaaaa aacgcgctta ccttaacgat aagcgcgatg ttgttcaagc
     7081 cttgagcggt tactgaaaaa acaccgcgat cttgttaaac atcgtcggat cggactgatt
     7141 acgctgcact ttcacgacat cttccagctt atcgatttgg cttatcatct gctccagacg
     7201 ctggtcgtca ttgaccagta gccagatatg gcttttgtcg ctgtcctgaa tcggcagaca
     7261 aagaatgcct tcaacgttaa aagcgcggcg ggcaaaaagg ccacaaacgt gggtcattac
     7321 gcccggatgg ttgcgaacgg tgagctccag aattacgttg tcatgagttg tgttttgcat
     7381 ggcttattcc cccaccattt cagtattcgc cgcacctggc ggcaccatcg ggtaaacttt
     7441 ttcttcggca tcaatgcgca catggatcag cgccgggcta gggcgattga tgatttcctg
     7501 caatgcagcc tgcggatcgg cttcgttatt caaatcacag gtttcgaggc cgaatccggc
     7561 ggcaatctgc ataaagttga ttttgcccgg ataggtggcg gcaaaaacgc cttgctcgta
     7621 gaacagactc tgttgctgat gcaccagccc cagcgcttcg ttgttcatca gaatgatttt
     7681 gacatccagc tgattttcac tggcggtcgc catctcctga atattcatca tcaggctgcc
     7741 gtcgccggag aaacacaaca ctttgcgatc cgggttcgcc agcgccgcgc caatcgccgc
     7801 aggcaggcca aaacccatcg tgcccagccc accggaggtc agccactggc gtgggcgatt
     7861 gagcggataa gcttgcgcgg tccacatctg atgctgaccc acatcggtgg tgataatcgc
     7921 attgtcatcg acacaggcgg caacggcgtt gatcaggccg taatggctta atggatcgca
     7981 cgctttcggg attggacacg ggaattcgcg ctgcaaatcc gctaccaact ggtgccactc
     8041 tgcacgcggt tgcgcttcca cctgcgggat caattgcgcc agcacgtcat caacatccgc
     8101 ctgaatcgcc acatgcggct gcttgatttt acccagctct gcgcgatcga tatcgacatg
     8161 aataattttg gcattcggac agaactgctc ggttttgcca atcgcccggt catcaaaacg
     8221 cgcaccgagc acaatcaaca aatccgcctc ctgcaaaata tagttggtac tgcgcacgcc
     8281 gtgcatcccc agcataccca gcgacaacgg atgcgctttt ggcagcatgc ccagcgccat
     8341 taaagtcatg gtggtaggca gttgcgcttt ctccgccagt tcacgcaccc gcgcaggcgc
     8401 attgatcaca ccaccaccca gataaagcac cgggcgtttg gcagcgttaa tcattgtagc
     8461 tacgtcacga atgctttctt cgctaaaggc gggtgcagcg gctttttctg ccaccgcggg
     8521 ctgagcttca atctcaaaaa ccgccgtttg tacatcctta ggaatgtcta tccacaccgg
     8581 gccagggcgg cctgattgcg caatgcggaa ggcatcgctc atgacctgcg ggagttcttc
     8641 gatatgtctg accagatagt tgtgtttggt gatggggata gagatgccgt aggtgtcgac
     8701 ttcctggaag gcgtcggtgc cgatcatcga ggcgggaacc tgaccagtga tgcaaatcag
     8761 cgggatggag tccagccgcg catcggcaat ggcggtcacc aggttagtcg cacccggtcc
     8821 gctacaggcc atacagaccg ccggtttacc gtcggtgcgc gccattccct gagcgataaa
     8881 gcccgcgccc tgttcatgac gggccagaat atggcggatt tgcgtgcttt ggcttaaggc
     8941 atcgtaaaca ggcaggatag aaccgcccgg aatgcctgtc acaatcttaa tgccctgctg
     9001 ttccaggaaa tgaacgataa attctgcgcc ggtaaagcgc ttacgcgtca atgttgtgcc
     9061 cgaacttgcc atgctccagt ccttttcttc tgggccgact ttccgggagg tgcttaaacg
     9121 aaaaaccccg cccggtttgc gccgacgggg ttttggaatc gtgtgttgtt ccagtcccta
     9181 cggcgcattg ccgacgacca ccaccacacg cacgacgacc actgcggcgg atggcgcagt
     9241 tggtagtagt tttgcgttga acatggaagt agtcattggg gaccttgttg gttttgtgtt
     9301 taacaatatt tatacaagca cagctttaca ggggagacaa tggaaaattt ttcagcaagg
     9361 gaaaattgag gggttgatca cgttttgtac tgaattgcag ataacaaaaa accccgccgg
     9421 agcgaggttt cgtcagtcgc ccgcggctgg taaccgcaaa gcacactgta ttatgtcaac
     9481 acagaaagta tacgtgttcc gcgcagaacg cgcaatttcg gcacgaattt tgacgtattt
     9541 agtgcatagt tgagtatcga tcacagtttg cgttttgtcc aaatattgct gtttatttat
     9601 acagtaaact tctataatat cactgtacgc aatgtgttat gcgggggccg catcgttacc
     9661 cggcgcacta agtcctggct gaaacgggtg gtgccgtcag cgccttaacc ccgcgtgagc
     9721 acactgtgtt atgtcaacaa gcacaaagtt tctccttgag ataccgcgtg cacaacagct
     9781 ggcaacaggc agcggaaagg tacgtcagct ggcagtgctc ctgaaccaca ggagacgcgt
     9841 atgagcctgg tggatatcgc cattcttatc ctcaaactca ttgttgcagc actgcaactg
     9901 cttgatgctg ttctgaaata cctgaagtaa ttcagattca agtcgcacca aaggggagcg
     9961 ggtaaccgct ccccttttat atttagcgtg cgggttggtg tcggatgcga tgctgacgta
    10021 tcttatccgc cctaccatct ctcccggcaa catttattgc cgcttttgtt tacatattct
    10081 gccgctaaac aactccccat tcctggcgta tatctggcta acattcatca atgtgataga
    10141 ttcctctccc gcatttatgg gaatgcgtag tgacttactc taattatttt tataaaagca
    10201 tccgtgataa tgaaaaggca tagaaacgtc aatttgttat tgatgttggt attactcgtg
    10261 gccgtcggtc agatggcgca aaccatttat attccagcta ttgccgatat ggcgcgcgat
    10321 ctcaacgtcc gtgaaggggc ggtgcagagc gtaatgggcg cttatctgct gacttacggt
    10381 gtctcacagc tgttttatgg cccgatttcc gaccgtgtgg gtcgccgacc ggtgatcctc
    10441 gtcggaatgt ccatttttat gctggcaacg ctggtcgcgg tcacgacctc cagtttgacg
    10501 gtattgattg ccgccagcgc gatgcagggg atgggcaccg gcgttggcgg cgtaatggcg
    10561 cgtactttgc cgcgagattt atatgaacgg acacagttgc gccatgctaa cagcctgtta
    10621 aacatgggga ttctcgtcag tccgttgctc gcaccgctaa tcggcggtct gctggatacg
    10681 atgtggaact ggcgcgcctg ttatctcttt ttgttggttc tttgtgctgg tgtgaccttc
    10741 agtatggccc gctggatgcc ggaaacgcgt ccggtcgatg caccgcgcac gcgcctgctt
    10801 accagttata aaacgctttt cggtaacagc ggttttaact gttatttgct gatgctgatt
    10861 ggcggtctgg ccgggattgc cgcctttgaa gcctgctccg gcgtgctgat gggcgcggtg
    10921 ttagggctga gcagtatgac ggtcagtatt ttgtttattc tgccgattcc agcagcgttt
    10981 tttggcgcat ggtttgccgg acgtcccaat aaacgcttct ccacgttaat gtggcagtcg
    11041 gttatctgct gcctgctggc tggcttgctg atgtggatcc ccgactggtt tggcgtgatg
    11101 aatgtctgga cgctgctcgt tcccgccgcg ctgttctttt tcggtgccgg gatgctgttt
    11161 cctctggcga ccagcggcgc gatggagccg ttccccttcc tggcgggcac ggctggcgcg
    11221 ctggtcggcg gtctgcaaaa cattggttcc ggcgtgctgg cgtcgctctc tgcgatgttg
    11281 ccgcaaaccg gtcagggcag cctagggttg ttgatgacct taatgggatt gttgatcgtg
    11341 ctgtgctggc taccgctggc gacgcggatg tcgcatcagg ggcagcccgt ttaagcgcac
    11401 gtcaccgcag catcgtcatc agctccatgg gagaacgatg ctgctttatc agatcacgca
    11461 tcacccgcat atgcggtgcg gagtaagaat aaaacgcctg atagcccgca caaagcacgc
    11521 tgtctggcgt ctcctgccga gcgtgcgggc acaggcgatg ccagtcgcac gcctggcagt
    11581 cacagcgcgg ttgcgggttt tcgctgccac accatttttg taaggtggcg tcaaaaagct
    11641 caatcgagat acgactgata tctgcccgca cccaacactc gaacagggca attaaaaatt
    11701 tcccccacag ctctgctgtc accgaagcgg ccgtcagatc gccccggtca tcaaattcaa
    11761 gatagggaat gagctgtacg gagcggatgc cccgctgttt gaaattgtga taaaaatcgg
    11821 caggttgcag agcatccttg tactctacaa ccagtttatg cctgtcctct tcagcatcaa
    11881 tgacctgact tcctgtcatg cgactctctc aataaatacg ataagttgat gtatatgcgt
    11941 tacagcaaac agtattgcga gagataacac cgcgagggag atcaaaaatt tgtcacggac
    12001 tacgtgaaat tgggaaaaat cgctattcgt gacatccatt aaattgcgat tacgggtgta
    12061 gtgccagagg atcagcgcca cgatggcgag aatgccaatc gaaatccaga ataacatgcc
    12121 cgcctggtgc cagttgtgtt tgatagccaa cgccatcaac gcgccgtagc ccagcatggt
    12181 acgaaaccac gccagtgatg tgcgctccgg ctgcagtcct gggtcggcaa tgcgtctggc
    12241 tttgcggcta tccggcatac aacaccagtc ccataacgat caccgccacg accatcaata
    12301 ttaagctaat gattaataag ctgttggtgt agggcaggtc ctctttcaga cgcatcgctt
    12361 tttcattgcg taaccagcgc aggtagccat acattgctaa cccgccggaa aacagacaca
    12421 acaacaacgc cagcagttca cgaatgacgg gcgtggcgaa atctggcgca agttgatcaa
    12481 gcccaacccc cgccgccaga aatcctagtg cggtacggat ccacgccaga aaagtacgct
    12541 cattagccag cgagaagcgg taatcgggcg cttctccaag gcgggaaatt ttcattgttg
    12601 ctcctttgca atttcactaa ctctctacct ttagcggttt tttttaagaa tttcgtccgc
    12661 ccgtttcctg atatgctttt gccatccgct acaaggaggt cagatgggta tcatagcgca
    12721 aaataaaatc agttcattgg gcatgctgtt cggtgccatc gctttaatga tggggattat
    12781 ccacttttca ttcggccctt tttccgcacc accgccaacg cttgaaagca tcgtggcgga
    12841 caaaacggct gaaattaaaa gaggtcttct cgccggaata aaaggcgaaa aaattaccac
    12901 cgtggagaaa aaagaggacg tagatgtcga taaaatcctc gatcaaagcg gtatcgcact
    12961 ggccattgct gcgctacttt gcgcatttgt tggcggtatg cgtaaggaaa atcgctgggg
    13021 cattcgcggc gcgctggttt ttggcggcgg aacgctggct ttccatacct tgctttttgg
    13081 tatcggcatc gtgtgcagca ttttgctgat attccttatc ttttccttcc tgaccggcgg
    13141 ttcgctggtt taagcagcct gaataatgcc cgccgggtga tcatcagcgg gcattatcgt
    13201 cagaacttct gttttttctc ttccaccttc accccttgtg tcggtaggcc ggtgtcatag
    13261 tcacgtacca ccggcgaata gccatcttgt gggcgtggac gaaatgcccc catccagcgc
    13321 ggccgtgcat ctttacgcca cggacgcagg ctccattgtt gacatcctcc acgccctgaa
    13381 ggacgtggcc ccctgctacg ttcaggctgt cgcctgaatc atttcggtgg gttcctgctt
    13441 caacgagcgg cctgactgca ccatctctcc acaggcaaga acggcgtgcc ccgccgctaa
    13501 aatgttacga gcgccattta catcggcgtt cgctgtatat ccacatacct ggcatctgaa
    13561 tttactttgt gacaggcgat tttctttcgc tgtatgacca cagcacacgc aacgctggct
    13621 tgtgtacgct ggcggaacag caagcacctg accaccacgc cagagctgct tgtacgcaag
    13681 ctggcggcgc atttcatacc agccctgatc cagtatcgaa cggtttaaac ctgattttgc
    13741 ccggacattg cgccccggca ggctgaccgt acccgctgct gactttgaca tgttgctgac
    13801 cttcaaatcc tcaatgacta tcattgcgtg gtttttgctg acggtcgttg tgactttgtg
    13861 aaggtagtcc ctgcggatat ttgcgataca ggaatgcagt cgctgtattt tgcgtttctg
    13921 cttctgccag ttgttgctga atttgacctt gcggcttaac tgtcgctgaa gtctcgccag
    13981 cttcttctgg tttttctgaa aactgtttac aggctcaaag actgtgccat ctgacagcgt
    14041 ggcgagttta gccacgccag catccagccc gaccattgat gctgaagggt gaaccggagt
    14101 tga
//
LOCUS       DADMKF010000105        13960 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.107, whole genome shotgun sequence.
ACCESSION   DADMKF010000105 DADMKF010000000
VERSION     DADMKF010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13960
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.107"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            <1..356
                     /locus_tag="JNC24_002855"
     CDS             <1..356
                     /locus_tag="JNC24_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAY9159553.1"
                     /translation="TPQDLTELQELLEKLQQAQEKGDMEQIINVNRLFRLAIYHRSNM
                     PILCEMIEQLWVRMGPGLHYLYEAINPAELREHIENYHLLLAALKAKDKEGCRHCLAE
                     IMQQNIAILYQQYNR"
     gene            complement(392..2494)
                     /locus_tag="JNC24_002856"
     CDS             complement(392..2494)
                     /locus_tag="JNC24_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HAY9159554.1"
                     /translation="MKIFSVRQTVLPALLALSPVVFAADEQTMIVSAAPQVVSELDTP
                     AAVSVVDGEEMRLATPRINLSESLTDVPGLQVQNRQNYAQDLQLSIRGFGSRSTYGIR
                     GIRLYVDGIPATMPDGQGQTSNIDLSSVQNVEVLRGPFSALYGNASGGVMNVTTQTGQ
                     QPPTIEASSYYGSFGSWRYGLKATGATGDGTQPGDVDYTVSTTRFTTHGYRDHSGAQK
                     NLANAKLGVRIDEASKLSLIFNSVDIKADDPGGLTKAEWKANPQQAPRAEQYDTRKTI
                     KQTQAGLRYERSLSAQDDMSVMMYAGERETTQYQSIPMAPQLNPSHAGGVITLQRHYQ
                     GIDSRWTHRGELGVPVTFTTGLNYENMSENRKGYNNFRLNSGMPEYGQKGELRRDERN
                     LMWNIDPYLQTQWQLSEKLSLDAGVRYSSVWFDSNDHYVTPGNGDDSGDASYHKWLPA
                     GSLKYAMTDAWNIYLAAGRGFETPTINELSYRADGQSGMNFGLKPSTNDTIEIGSKTR
                     IGDGLLSLALFQTDTDDEIVVDSSSGGRTTYKNAGKTRRQGAELAWDQRFAGDFRVNA
                     SWTWLDATYRSNVCNEQDCNGNRMPGIARNMGFASIGYVPEDGWYAGTEARYMGDIMA
                     DDENTAKAPSYTLVGLFTGYKYNYHNLTVDLFGRVDNLFDKEYVGSVIVNESNGRYYE
                     PSPGRNYGVGMNIAWRFE"
     gene            2736..3797
                     /locus_tag="JNC24_002857"
     CDS             2736..3797
                     /locus_tag="JNC24_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HAY9159555.1"
                     /translation="MHLRHLFSSRLRGSLLLGSLLVASSFSTQAAEEMLRKAVGKGAY
                     EMAYSQQENALWLATSQSRKLDKGGVVYRLDPVTLEVTQAIHNDLKPFGVTINNTTQT
                     LWFGNTVNSAVTAIDAKTGEVKGRLVLDDRKRTEEVRPLQPRELVADDATNTVYISGI
                     GKESVIWVVDGENIKLKTAIQNTGKMSTGLALDSKGKRLYTTNADGELITIDTADNKI
                     LSRKKLLDDGKEHFFINISLDTARQRAFITDSKAAEVLVVDTRNGNILAKVAAPESLA
                     VLFNPARNEAYVTHRQAGKVSVIDAKSYKVVKTFDTPTHPNSLALSADGKTLYVSVKQ
                     KSTKQQEATQPDDVIRIAL"
     gene            complement(3912..5411)
                     /gene="ansP"
                     /locus_tag="JNC24_002858"
     CDS             complement(3912..5411)
                     /gene="ansP"
                     /locus_tag="JNC24_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparagine permease"
                     /protein_id="HAY9159556.1"
                     /translation="MSKHDTDTSDQHAAKRRWLNAHEEGYHKAMGNRQVQMIAIGGAI
                     GTGLFLGAGARLQMAGPALALVYLICGLFSFFILRALGELVLHRPSSGSFVSYAREFL
                     GEKAAYVAGWMYFINWAMTGIVDITAVALYMHYWGAFGGVPQWVFALAALTIVGTMNM
                     IGVKWFAEMEFWFALIKVLAIVTFLVVGTVFLGSGQPLDGNTTGFHLITDNGGFFPHG
                     LLPALVLIQGVVFAFASIEMVGTAAGECKDPQTMVPKAINSVIWRIGLFYVGSVVLLV
                     MLLPWSAYQAGQSPFVTFFSKLGVPYIGSIMNIVVLTAALSSLNSGLYCTGRILRSMA
                     MGGSAPSFMAKMSRQHVPYAGILATLVVYVVGVFLNYLVPSRVFEIVLNFASLGIIAS
                     WAFIIVCQMRLRKAIKEGKAADVSFKLPGAPFTSWLTLLFLLSVLVLMAFDYPNGTYT
                     IAALPIIGILLVIGWFGVRKRVAEIHSTAPVVEEDEEKQEIVFKPETAS"
     gene            5678..6295
                     /locus_tag="JNC24_002859"
     CDS             5678..6295
                     /locus_tag="JNC24_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HAY9159557.1"
                     /translation="MIKVYGVPGWGSTISELMLTLADIPYQFVDVSGFDHEGTSRELL
                     KTLNPLCQIPTLALENDEIMTETAAIALMVLDRRPDLAPPVGRAERQQFQRLLVWLVA
                     NVYPTFTFADYPERWAPDAPEQLKKNVIEYRKSLYIWLNSQLTAEPYAFGEQLTLVDC
                     YLCTMRTWGSGHEWFQDNATNISAIADAVCQLPKLQEVLKRNEII"
     gene            6371..6583
                     /locus_tag="JNC24_002860"
     CDS             6371..6583
                     /locus_tag="JNC24_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncH family protein"
                     /protein_id="HAY9159558.1"
                     /translation="MLCFLIYITLPFSQLVYFISSEKKLTIHIVQMFHLLSQVFYNLK
                     KFLMMDMLGVGDAININANKNIRQVC"
     gene            7404..9512
                     /locus_tag="JNC24_002861"
     CDS             7404..9512
                     /locus_tag="JNC24_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HAY9159559.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTVWQGDDVQRRVKGVVTWFELGENDKNQMLYSMKVCPPLWRI
                     GLRQNFRIFQNEDIESILATILKENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAQKSTDQSLVLCDTVRYLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWPGRFDQEGQYQDYQRTQYEVYDYPGRFKGAHGQNFARWQ
                     MDGWRNNAEVARGTSRSPEIWPGRRIVLTGHPQANLNREWQVVASDLHGEQPQAVPGR
                     RGSGTTLENHFAVIPADRTWRPQPLMKPLVDGPQSAVVTGPAGEEIFCDEHGRVRVKF
                     NWDRYNPSNQDSSCWIRVAQAWAGTGFGNLAIPRVGQEVIVDFLNGDPDQPIIMGRTY
                     HQENRTPGSLPGTKTQMTIRSKTYKGSGFNELKFDDATGKEQVYIHAQKNMNTEVLNN
                     RTTDVINNHAETIGNNQMIAVTNNQIETVGVNQIETVGSNQIIKVGSVQVETIGLVRA
                     LTVGVAYQTTVGGIMNTSVALMQSSQIGLHKSLRVGLGYDVKVGNNVTFTVGKTKKDD
                     TGQTAIYSAGEHLELCCGKARLVLTKDGQIFLNGTKIHLQGKEQVNGDSLLINWNCAA
                     SKSPPKTPDEKQDTPDMREY"
     gene            9580..10238
                     /locus_tag="JNC24_002862"
                     /pseudo
     CDS             9580..10238
                     /locus_tag="JNC24_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001375079.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Rhs"
     gene            10465..13575
                     /locus_tag="JNC24_002863"
     CDS             10465..13575
                     /locus_tag="JNC24_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005142637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HAY9159560.1"
                     /translation="MHDPAYPESLPAAPLVRYTYTEAGELLAVYDRSNTQVRAFTYDA
                     QHPGRMVAHRYAGRPEMRYRYDDTGRVVEQLNPAGLSYRYLYEQDRITVTDSLNRREV
                     LHTEGGAGLKRVVKKELADGSVTRSGYDAAGRLTAQTDAAGRRTEYGLNVVSGDITDI
                     TTPDGRETKFYYNDGNQLTAVVYPDGLESRREYDEPGRLVSETSRSGETVRYRYDDAH
                     SELPATTTDATGSTRQMTWSRYGQLLAFTDCSGYQTRYEYDRFGQMTAVHREEGISLY
                     RHYDNRGRLTSVKDAQGRETQYEYNAAGDLTAVITPDGNRSETQYDAWGKAVSTTQGG
                     LTRSMEYDAAGRVISLTNENGSHSDFSYDALDRLVQQGGFDGRTQRYHYDLTGKLTQS
                     EDEGLVTLWHYDASDRITHRTVNGDPAEQWQYDGHGWLREISHLSEGHRVAVHYGYDD
                     KGRLTGERQTVENPETGELLWQHETKHAYNEQGLANRVTPDSLPPVEWLTYGSGYLAG
                     MKLGGTPLVEYTRDRLHRETVRTLAPDLDIRIPYATDPAGNRLPDPELHPDSTLTAWP
                     DNRIAEDAHYVYHYDEYGRLTEKTDRIPTGVIRTDDERTHHYDSQHRLVFHTRIQHGE
                     PLVESRYLYDPLGRRMAKRVWRRERDLTGWMSLSRKPEETWYGWDGDRLTTVQTDTTR
                     IQTVYEPGSFTPLIRVETENGEREKAQRRSLAEKLQQEGSEDGHGVVFPAELVRLLDR
                     LEEEIRADRVSSESRVWLAQCGLTVEQLARQVEPEYTPVRNVHLYHCDHRGQPLALIS
                     EDGDTVWSGAYDEWGNQLNEENPHHVYQPYRLPGQQYDEESGLYYNRHRYYDPLQRRY
                     ITQDPIGLKGGWNLYQYPLNPVSRIDPLGLFSCESCSHFIDDINNKLDNLPFNPTKMF
                     TASLNTANSARLYASGMTKFGISVAADGTIIAAPVGAASMALGAWNIKSAIAAYNRAN
                     LQLSESLQENGRDRCWRNLLGILPYGEQFDDPGEPSILDVYGEKIENIKDSPMKFFEE
                     LGTLGL"
BASE COUNT         3579 a         3447 c         3808 g         3126 t
ORIGIN      
        1 tcaccccgca agacctgacg gaactccagg aattgctcga gaaattacag caggcgcaag
       61 aaaagggcga catggaacaa atcattaatg taaacaggct atttcgctta gcgatttatc
      121 atcgctcaaa tatgcccatc ctgtgtgaga tgattgagca actgtgggtc aggatggggc
      181 ctggtttaca ttatctctat gaagcgatta atccagcgga attacgggag catatagaaa
      241 actatcatct gttactcgct gcgttaaaag caaaagacaa agagggatgc agacattgtc
      301 ttgctgaaat tatgcaacaa aatattgcta ttttatacca gcaatacaat cgttaaagta
      361 aatgcccggc aaataccggg catcaaggta attactcaaa tctccacgca atattcatgc
      421 cgacaccata atttcgtccg ggcgaaggtt cgtaatatcg cccgtttgac tcattgacaa
      481 tgacagaacc aacgtattct ttatcgaata aattatcgac acgaccaaat aaatccacag
      541 ttaaattgtg gtaattgtat ttatacccgg tgaataagcc gacgagagta taagacggcg
      601 cttttgccgt attttcatca tctgccataa tatcgcccat ataacgcgct tccgtgcctg
      661 cataccaacc atcttccggt acataaccta tcgacgcaaa gcccatatta cgggcgatcc
      721 ctggcatccg attaccgtta caatcctgtt cattgcaaac attgctgcga taggtcgcat
      781 caagccaggt ccaggacgcg tttacgcgaa aatctcctgc gaaacgttga tcccatgcca
      841 gttcagcgcc ttgacgacgg gtctttccgg catttttgta agtcgtacgc ccaccgctac
      901 tgctatcgac aacaatttca tcatcagtgt cggtctgaaa caatgcgaga ctaagcagcc
      961 catcaccaat acgcgtttta ctgccgatct caattgtatc gttggtggat ggttttaaac
     1021 caaagttcat accgctttgc ccatcagcac gataagacag ctcattaatc gtcggcgttt
     1081 caaaacctcg cccggctgcc agatagatat tccaggcatc ggtcattgca tattttaacg
     1141 aaccggcagg tagccattta tgataactgg catcaccgct gtcatcgccg ttacccggag
     1201 taacgtaatg gtcgttggaa tcaaaccaca cggagctgta gcgcacgcca gcatccagcg
     1261 acagtttttc gctcagctgc cactgcgtct gtaaataggg atcgatgttc cacatcagat
     1321 tgcgttcgtc gcgacgcaac tcaccttttt gcccgtactc cggcatgccg ctattcaggc
     1381 ggaagttatt gtagcccttg cggttttcac tcatgttttc gtagttcagg ccggtagtga
     1441 acgtgaccgg aacgcccagt tcaccacggt gtgtccagcg gctgtctatc ccctggtaat
     1501 ggcgttgcag ggtaatcacg ccgcccgcat gtgacgggtt aagttgtggt gccatgggta
     1561 ttgactggta ctgggtcgtt tctcgctctc cggcatacat catcacactc atatcatctt
     1621 gcgcgctcag gctacgctca tagcgcaacc cagcctgagt ttgcttgatg gtttttcgcg
     1681 tgtcgtactg ttctgcacga ggcgcttgtt gtggattagc cttccattct gctttggtta
     1741 gcccacccgg gtcatctgct ttgatatcca cactattgaa aatcagactt aatttgctgg
     1801 cttcatcaat gcgtacgccc agtttggcat tggctaaatt tttctgtgcg ccactatggt
     1861 cacgatagcc gtgggtcgta aaacgcgtgg ttgagacggt gtaatcgaca tcgccaggct
     1921 gtgtgccgtc tcccgttgcg cccgttgctt tcagcccata gcgccagctg ccaaaactgc
     1981 cgtagtaact actggcttca atggttggtg gctgttgtcc ggtctgggtg gtgacattca
     2041 ttaccccacc agacgcgttg ccatacaggg cagagaaggg gccacgcagc acttccacat
     2101 tttgcacact gcttaaatcg atgttggatg tttgcccttg cccgtcgggc atggtggcgg
     2161 gaataccgtc cacatacagg cgaataccgc gaataccgta agtggagcgg gagccaaatc
     2221 cgcgaatcga cagctgtaaa tcttgcgcat agttctgccg gttttgtacc tgcaaaccag
     2281 gcacgtcggt cagtgattcg gacaagttaa tgcgcggtgt tgccaggcgc atctcctcgc
     2341 catccaccac gcttactgct gctggggtat ccagttctga aaccacctgc ggtgcggcac
     2401 tgacaatcat ggtctgttca tcagcggcaa aaacaacggg ggaaagggca agtagtgcgg
     2461 gcaaaacggt ctgtcggacg gaaaaaatct tcatgaaaaa agccaggtta agaatgggaa
     2521 aacgccgtta tggtaataaa attgtaaatt catggaaaac gaaacggcac gatacgttaa
     2581 gtaattgaga aaattgtagt cgtaacggca agaaatgctc cacatttgag aaaataatga
     2641 ttaccattcc catttataac aagagcgtaa cgatgattac gcttagcgaa gcattgtgaa
     2701 gcagcaaaaa tatcggttca tcaaagggag tcgtcatgca tttacgtcat ctgttttcat
     2761 cgcgcctgcg tggttcatta ctgttaggtt cattgcttgt tgcttcatca ttcagtacgc
     2821 aggccgcaga agaaatgctg cgtaaagcgg taggtaaagg tgcctacgaa atggcttata
     2881 gccagcaaga aaacgcgctg tggctcgcca cttcgcaaag ccgcaaactg gataaaggcg
     2941 gcgtggttta tcgtcttgat ccggtcactc tggaagtgac gcaggcgatc cataacgatc
     3001 tcaagccgtt tggtgtcacc atcaataaca cgactcagac actgtggttt ggtaacactg
     3061 taaacagcgc ggtcacggcg atagatgcca aaacgggcga ggtgaaaggc cgtctggtgc
     3121 tggatgatcg taagcgtacg gaagaggtgc gcccgctgca accgcgtgag ctggtagctg
     3181 acgatgccac gaacaccgtt tacatcagtg gtattggtaa agagagcgtg atttgggtcg
     3241 ttgatggcga gaatatcaaa ctgaaaaccg ccatccagaa caccggtaaa atgagtaccg
     3301 gtctggcgct tgatagcaaa ggcaaacgtc tttacaccac taacgctgac ggcgaattga
     3361 ttaccatcga caccgccgac aataaaatcc tcagccgtaa aaagctgctg gatgacggca
     3421 aagagcactt ctttatcaac attagccttg ataccgctag gcagcgtgca tttatcaccg
     3481 attctaaagc cgcagaagtg ttagtggtcg atacccgtaa tggcaatatt ctggcgaagg
     3541 ttgcagcacc ggaatcactg gctgtgctgt ttaacccagc gcgtaatgaa gcctacgtaa
     3601 cgcatcgtca ggcaggtaaa gtcagtgtga ttgacgcgaa aagctataaa gtagtgaaaa
     3661 cgttcgatac gccgactcat ccaaacagcc tggcgctgtc tgccgatggc aaaacgctgt
     3721 atgtcagtgt gaaacaaaaa tccactaaac agcaggaagc tacccagcca gacgatgtga
     3781 ttcgtattgc gctgtaatag atatggcgag ggtgaaaacg gagagacttg cctgactttt
     3841 atcacgccgc atgcggcaca ccttcgtcag taatctaaat ttgcccggca cgtagccggg
     3901 caattttgca attagctcgc cgtttcaggc ttaaacacaa tttcctgttt ttcttcatct
     3961 tcctcgacga ctggcgcagt gctgtgaatt tcagcaacgc gtttgcgcac accaaaccag
     4021 cctataacca gcagaatacc gataatcggc agcgccgcga tagtataagt cccgttcggg
     4081 taatcgaacg ccatcagcac aaggacactc agtaaaaaca gtaatgtcag ccaggaagtg
     4141 aagggcgcgc caggcagttt aaaactgaca tccgctgctt tgccttcttt aatcgcttta
     4201 cgcaggcgca tctggcacac gatgataaac gcccatgaag cgatgattcc cagcgacgcg
     4261 aagttcaaca caatctcaaa tacgcgcgac ggcaccagat agttgaggaa tacgccgacg
     4321 acatacacaa ccagtgtcgc cagaatcccg gcatacggca catgctgacg actcattttc
     4381 gccataaaac tcggtgcgga accgcccatc gccatcgagc gcagaatacg cccggtgcag
     4441 tacagacctg agttcaggct ggagagggca gcggtcagca ccacaatgtt cataatgctg
     4501 ccgatatatg gcacacccag tttagagaaa aacgtcacga acggactttg ccccgcctga
     4561 tacgcgctcc acggcaataa cataaccagc aacaccacgg agccgacgta aaacaggcca
     4621 atacgccaaa tcacactgtt aatggcttta ggcaccatgg tctgcggatc tttacattct
     4681 ccggcagctg tacccaccat ttcaatggag gcaaaagcaa acactacgcc ctgaatcaac
     4741 accagcgcag gcagcaaacc gtgggggaag aagccgccat tatcggtgat taaatgaaag
     4801 ccagtggtgt tgccatccag cggctgacca ctaccgagga acactgtacc cacgaccaaa
     4861 aaggtcacaa tggcgagcac tttaataagc gcaaaccaga actccatctc cgcaaaccat
     4921 ttcacaccga tcatattcat ggtgccaacg atggtaagtg cagcgagcgc aaagacccac
     4981 tgcggcacgc cgccaaacgc accccagtaa tgcatataca gagcgacggc ggtaatatca
     5041 acaatccccg tcatcgccca gttgatgaag tacatccagc cagcaacata agcggctttc
     5101 tcacccaaaa actcacgggc ataagagaca aaactgccac tggaagggcg gtgtagcacc
     5161 agctcaccca atgcacgcag aataaaaaac gaaaacaagc cacaaattaa ataaaccagt
     5221 gccagtgctg gacccgccat ttgcagtcgg gctcctgcac ctaaaaacaa gccggtgcca
     5281 atcgcgccgc caatggcgat catctgtacc tggcgattgc ccatcgcttt gtgatacccc
     5341 tcttcgtggg cattaagcca gcggcgtttc gcggcgtgtt gatctgaagt gtcggtgtcg
     5401 tgtttactca ttgctctccc tgattgcttt aatgaaaaag tcatataagt tgccatgaac
     5461 aatgtttatt catcctggcg atgatagtta tcaccattat tctttatggg tataaaacta
     5521 tcggcgcagc atcctacccg ttatcgatat acgatgcaaa acatcccctt acaatcctga
     5581 aggggattca tacaactgac gaaaaaatga caaatacttt tgctggttaa cctgtgtact
     5641 gtcctacact taatctttaa aagattgtga ggggcatatg attaaggtgt atggcgtacc
     5701 cggatggggc tctacaatca gtgagctgat gctaacactg gctgatattc cttatcagtt
     5761 cgttgatgtc agtggttttg accacgaggg aacttcacgc gagttattga aaaccctgaa
     5821 cccgttgtgt cagattccga cactggcgct ggaaaatgac gagattatga cggagacagc
     5881 ggcgattgca ttgatggttc tcgatcgtcg cccggacctt gcgccaccgg ttgggcgcgc
     5941 cgaacgtcag cagtttcaac ggctattggt ctggctggtt gctaatgtct atccaacatt
     6001 cactttcgcc gattaccctg agcgttgggc tcctgacgca cctgaacagt taaagaagaa
     6061 tgttattgaa tatcgtaaat cgctttatat ctggctgaat tcacaattga ccgctgaacc
     6121 ttatgcgttt ggtgagcaac taacgctggt ggactgttat ctttgcacta tgcgcacatg
     6181 ggggtctggg catgaatggt ttcaggataa tgccacgaat atcagtgcga ttgctgatgc
     6241 tgtatgtcag ttaccgaaat tacaagaagt attaaaaagg aatgaaatta tttgagtggg
     6301 ataccagagg attatgttat ttattattca gtaactttat ccacaatgat gggcgtaatt
     6361 aattaaattc atgttatgtt ttttgattta tatcactctc cccttcagtc agcttgtcta
     6421 tttcatttcc tctgaaaaga aactaactat tcacattgtg cagatgtttc acttgttatc
     6481 acaggtattc tataatctca aaaagttttt aatgatggat atgctcggag ttggagatgc
     6541 aattaatatt aatgcaaata aaaatatccg gcaggtatgc taaaaggata aatggcgttc
     6601 tctgtattta ttcgcaatta tctataggat aaatatggag agcttgatga gtcagagcta
     6661 aaacagcaat tgataaataa atatgttgat ttattcaaga aaatggatgt attttaaata
     6721 ttgattctta atataaccta atcaatagat tatcacgtga gagctgcaca ttattggcgg
     6781 ggccggggaa aacatcattt tgggaacaat gaatcaggta ctcttaccac tgaagcaaga
     6841 tcatccgggc accaggtggt ggtgcagtgc tttggaaatg gtcctctcgg ggggagttgc
     6901 cattattttc tatgctattt gcttattata ttcatcaggt gaatttaata aattcctaat
     6961 ttgaaatatc cactattaag ccagtgttta acgacgttaa gatggcaatg tgcagatcat
     7021 gttatcaggg tgggcggtag cgtatattcc ttatacaaac catcattcat ttcaatgatt
     7081 gttattgaat tatttattgt tgtgaataaa aagacattat gaatattggt ttgactttgc
     7141 atatcaggtt ttttattact cgctgtaatg tacgagcctg tcgtgaataa cgtcgagaac
     7201 ccatccgttg cgtttgtgat tattttgttg actaaacaga cacccgtttc tctgaagtaa
     7261 aatcccagac taaatcatca cataaccatg acatttttct gatattcccc ggtaacgcca
     7321 gatgtcgact cgcttatctc atccgcccga catgttgtac atgcaatatg tgcggattat
     7381 tattcatttt cacggaggtt gctatgtcaa ccggattacg tttcacactg gaagtggacg
     7441 gcctgccacc ggatgctttt gcggtggttt cctttcattt gaaccagtca ctctcttcgc
     7501 tcttttccct cgatctctct ctggtcagcc agcagtttct ctcccttgaa tttgcgcagg
     7561 tgctggacaa aatggcctac ctgacggtat ggcagggcga tgacgtacag cgccgggtga
     7621 aaggtgtggt gacctggttt gaactggggg agaacgacaa aaaccagatg ctgtacagca
     7681 tgaaggtatg cccaccgctg tggcgcatag ggctgcgcca gaacttccgt atcttccaga
     7741 atgaggacat cgaaagcatc ctcgcgacga tcctgaagga aaacggtgtg accgagtgga
     7801 gtccgctgtt cagcgagccg catccttccc gtgagttttg tgtccagtac ggcgaaactg
     7861 attacgattt cctgtgccgg atggcggcgg aggaaggcat cttcttttat gaggagcacg
     7921 cgcaaaaaag taccgaccag agcctggtcc tgtgcgacac cgtgcgttat ctgccggagt
     7981 cctttgagat cccctggaac ccgaacaccc gtaccgaggt aagtaccctc tgcatcagcc
     8041 agtttcgcta cagcgcacaa atccgtcctt cttccgtagt gaccaaagac tacaccttta
     8101 aacgtcccgg ctggccaggg cgttttgatc aggaaggcca gtaccaggat taccagcgca
     8161 cgcagtatga agtgtatgac taccccggac gtttcaaggg tgcccacggg cagaactttg
     8221 cccgctggca gatggacggc tggcgaaaca acgcagaagt ggcgcgcgga acaagccgtt
     8281 cgccggagat atggccggga cggcgaattg tgctgacggg gcatccgcag gcgaacctga
     8341 accgggaatg gcaggtggtg gcgagcgatc ttcacggcga acagccgcag gcggtaccgg
     8401 gacgcagggg ttcaggcacc acgctggaga accattttgc ggtaatcccg gcagacagaa
     8461 catggcggcc acagccgttg atgaaaccgc tggtggatgg cccgcagagc gccgtcgtga
     8521 cgggaccggc aggcgaggaa atcttctgtg atgaacatgg ccgcgtgcgg gtgaaattta
     8581 actgggaccg ctataacccg tcaaaccagg acagttcatg ctggatccgt gtggcacagg
     8641 cgtgggcagg caccggcttt ggcaacctgg cgataccgcg tgtgggtcag gaggtgattg
     8701 tggacttcct caacggcgac ccggaccagc cgattattat ggggcgtacc taccaccagg
     8761 aaaaccgcac acccggcagc ctgccgggga cgaagacgca gatgaccatt cgttcgaaaa
     8821 cctataaggg cagcgggttt aatgaactga agtttgacga tgcgacaggg aaagaacagg
     8881 tctacatcca cgcgcagaag aacatgaaca ccgaggtgct gaataaccgc accacggatg
     8941 tgataaacaa ccatgctgaa accattggca acaatcagat gattgcggtt accaacaatc
     9001 agatagagac ggtgggcgtt aaccagatag agacggtggg cagtaaccag ataattaaag
     9061 tgggttctgt tcaggtggaa acgattggac ttgttcgtgc gctgacggtg ggcgtggcgt
     9121 atcagacgac ggtaggtggc attatgaaca cctcggtggc actgatgcag tcctcgcaga
     9181 tcggtttgca taaatcgttg agggtcgggc tgggttatga cgtcaaagtc ggaaataacg
     9241 ttaccttcac cgttggtaaa acgaaaaagg atgataccgg gcagaccgcg atttactccg
     9301 ccggtgagca tctggagctc tgctgtggta aggcaaggct ggtgctgacg aaggacggac
     9361 aaatttttct caacggcaca aaaattcatt tgcagggtaa ggagcaggtt aatggtgact
     9421 cactgttgat taactggaac tgtgcagcct cgaaatcccc accgaagacc cctgatgaaa
     9481 agcaggatac gccggatatg agagagtact gatcccgtac ctgttcggta ttcagtaaca
     9541 aaccagcatt aaaaataaac agaagaagga atttgaatca tgagcggaaa accagcggcg
     9601 cgtcagggag atatgactca gtatggcggt cccattgtcc agggttcggc aggtgtaaga
     9661 attggcgcgc ccaccggcgt ggcgtgctcg gtgtgtccgg gcgggatgac ttcgggcaac
     9721 ccggtaaatc cgctgctggg ggcgaaggtg ctgcccggcg agacggacct tgcgctgccc
     9781 ggcccgctgc cgttcattct ctcccgcacc tacagcagct accggacgaa gacgcctgca
     9841 ccggtgggcg ttttcggccc cggctggaaa gcgccttctg atatccgctt acagctacgt
     9901 gatgacggac tgatactcaa cgacaacggc gggcggagca ttcactttga gccgctgctg
     9961 ccgggggagg cggtgtacag ccgcagtgag tcaatgtggc tggtgcgcgg tggtaaggca
    10021 gcacagccgg acggccatac gctggcgcgg ctgtgggggg cgctgcgccg gatatccggt
    10081 taagcccgca tctttacctg gcgaccaaca gcgcacaggg gccgtggtgg atactggggg
    10141 gggctgagcg ggtgccgggt gctgaggacg tactgccagc gccgctgccg ccgtaccggg
    10201 tgcttaccgg gatggcggac gctgacgtac cggcgtgagg ccgccggtga cctggccggg
    10261 gaaatcaccg acgtgacgga cggtgccggg cgggagttcc gtctggtgct gaccacgcag
    10321 gcgcagcgtg cggaagaggc ccgcacctct tcgctatctt cttctgacag ttcccgccct
    10381 ctctcagcct cagcgttccc cgacacactg cccggtaccg aatacggccc cgacaggggt
    10441 atccgccttt cggcggtgtg gttgatgcac gacccggcat acccggagag cctgcccgct
    10501 gcgccactgg tgcggtacac gtatacggaa gccggtgaac tgctggcggt atatgaccgc
    10561 agcaatacgc aggtgcgcgc tttcacgtat gacgcgcagc acccgggccg gatggtggcg
    10621 caccgttacg cgggaaggcc ggagatgcgc taccgctacg acgatacggg gcgggtggtg
    10681 gagcaactga acccggcagg gttaagctac cgctatcttt atgagcagga ccgcatcacc
    10741 gtcaccgaca gcctgaaccg gcgtgaggtg ctgcatacag aaggcggggc cgggctgaaa
    10801 cgggtggtga aaaaagaact ggcggacggc agcgtcacgc gcagcgggta tgacgcggca
    10861 ggaaggctca cggcgcagac ggacgcggcg ggacggcgga cagagtacgg tctgaatgtg
    10921 gtgtccggcg atatcacgga catcaccaca ccggacgggc gggagacgaa attttactat
    10981 aacgacggga accagctgac ggcggtggtg tacccggacg ggctggagag ccgccgggaa
    11041 tatgatgaac cgggcaggct ggtatcggag acatcgcgca gcggggagac agtacgctac
    11101 cgctacgatg acgcgcacag tgagttaccg gcgacgacaa cggatgcgac gggcagcacc
    11161 cggcagatga cgtggagccg ctacgggcag ttgctggcgt tcactgactg ctcgggctac
    11221 cagacccgct atgaatacga ccgcttcggc cagatgacgg cggttcaccg cgaggaaggt
    11281 atcagccttt accgccacta tgacaaccgt ggccggttaa cctcggtgaa agacgcacag
    11341 ggccgtgaaa cgcagtatga gtacaacgcc gcaggcgacc tgactgccgt tatcaccccg
    11401 gacggcaacc ggagcgagac acagtacgat gcgtggggaa aggcggtcag caccacgcag
    11461 ggcgggctga cgcgcagtat ggagtacgat gctgccggac gtgtcatcag tctgactaac
    11521 gagaacggca gtcacagcga cttcagctac gatgcgctgg accggctggt acagcagggc
    11581 ggctttgacg ggcggacgca acgttatcat tatgacctga ccggaaaact cacgcagagt
    11641 gaggatgagg ggcttgtcac cctctggcac tacgatgcgt cggaccgcat cacgcaccgg
    11701 acggtgaacg gcgacccggc agagcagtgg cagtatgacg gccacggctg gctgagagaa
    11761 atcagccacc tgagcgaagg ccaccgtgtc gctgtccact acggctacga cgataagggc
    11821 cgcctgacgg gcgaacggca gacggtggag aacccggaga cgggggaact gctgtggcag
    11881 catgagacga aacacgccta caacgaacag gggctggcaa accgcgtcac gccggacagc
    11941 ctgccgccgg tggagtggct gacctacggc agcggttacc tggcgggcat gaagctgggc
    12001 gggacgccgc tggtcgagta tacgcgggac aggctgcacc gtgagacggt gcgcaccctc
    12061 gcaccggacc tggacatccg catcccgtat gccaccgacc cggcgggaaa ccgcctgccg
    12121 gacccggagc tacacccgga cagcacgctc acggcgtggc cggataaccg catcgcagag
    12181 gatgcgcact atgtttacca ctatgatgaa tacggcagac tgacggagaa gacggaccgc
    12241 atcccgacgg gtgtgatacg gacggacgac gagcggaccc accactacga cagccagcac
    12301 cgcctggtgt tccacacgcg gatacagcat ggcgagccac tggtcgagag ccgctacctc
    12361 tacgacccgc tgggacggcg aatggcgaaa cgggtatggc ggcgggagcg tgacctgacg
    12421 gggtggatgt cgctgtcgcg taaaccggag gagacatggt acggctggga cggcgacagg
    12481 ctgacgacgg tacagaccga caccacacgt atccagacgg tatacgagcc gggaagcttc
    12541 acgccgctca tccgggtcga gacagagaac ggcgagcggg agaaagcgca gcgccgcagc
    12601 ctggcggaga agctgcagca ggaagggagc gaggacgggc acggcgtggt atttccggct
    12661 gaactggtgc ggctgctgga cagactggag gaagaaatcc gggcagaccg cgtgagcagt
    12721 gaaagccggg tgtggcttgc gcagtgcggg ctgacggtgg agcaactggc cagacaggtg
    12781 gagccggaat acacaccagt gcgaaacgtt catctttacc actgcgacca tcggggccag
    12841 ccgctggcgc tcatcagcga agacggcgat acggtgtgga gcggggcgta tgatgagtgg
    12901 ggcaaccagc taaatgagga gaacccgcat catgtgtatc agccataccg tctgccgggg
    12961 cagcagtatg atgaggagtc ggggctgtac tacaatcgtc accggtacta tgatccgttg
    13021 cagagacggt acatcaccca ggaccccatt gggctgaaag gtgggtggaa tctgtatcag
    13081 tatccgctga atccggtgag caggatagat ccgttggggt tattctcatg tgagagttgt
    13141 agtcatttta tagatgatat aaataataaa ctggataacc ttccgtttaa cccgacaaaa
    13201 atgtttacag ctagtttaaa tactgctaat tcagcaaggc tgtatgcaag tggcatgaca
    13261 aaattcggta taagtgttgc tgccgatgga acgatcattg ctgccccagt cggtgcagct
    13321 tctatggctt taggggcgtg gaatataaaa tctgcgatag ctgcatataa cagagcaaat
    13381 ttacaattat cagaatcact tcaggaaaat ggtagagata ggtgttggag aaatttattg
    13441 ggtattttac cttatggtga gcagtttgat gaccctggtg aaccatcaat attagacgtt
    13501 tatggtgaaa agattgagaa tatcaaagat tctccaatga agttttttga agagcttggg
    13561 acgctggggt tgtaatgtgt atttttaatg atatttttat gtttgtgttt attgttagta
    13621 gtatacttgt aaccgtgcat tttgtgtatt ctttattttt taaaagtgga aaggtaaaag
    13681 ataaggtaaa aaaatggatt aaagtaatgt gtgatttatt ttgggggata ggatagtggt
    13741 gtgatatgga taaatataaa ttacagtggc tggcatattt cgacctgacg ccggagtaga
    13801 taaaaaaaca gacaggattg gagtatagca agcgtctgac aatctctcgg gacagtagta
    13861 tgatgaagag gcggggttgt actataaccg taaccggtac tacgatccgc taaagggcaa
    13921 gtatatgact cagggaaaga tcatcagaag ttcacttttt
//
LOCUS       DADMKF010000106        13905 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.108, whole genome shotgun sequence.
ACCESSION   DADMKF010000106 DADMKF010000000
VERSION     DADMKF010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13905
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.108"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            242..1495
                     /gene="kch"
                     /locus_tag="JNC24_002864"
     CDS             242..1495
                     /gene="kch"
                     /locus_tag="JNC24_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HAY9159561.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISIIISGITVFATSMT
                     SIFGPLIRGGFNKLVKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESVQK"
     gene            complement(1550..1723)
                     /locus_tag="JNC24_002865"
     CDS             complement(1550..1723)
                     /locus_tag="JNC24_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HAY9159562.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            1866..3326
                     /gene="cls"
                     /locus_tag="JNC24_002866"
     CDS             1866..3326
                     /gene="cls"
                     /locus_tag="JNC24_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAY9159563.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAR
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            3361..3690
                     /locus_tag="JNC24_002867"
     CDS             3361..3690
                     /locus_tag="JNC24_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciU family protein"
                     /protein_id="HAY9159564.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDIFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(3743..4747)
                     /gene="oppF"
                     /locus_tag="JNC24_002868"
     CDS             complement(3743..4747)
                     /gene="oppF"
                     /locus_tag="JNC24_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppF"
                     /protein_id="HAY9159565.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATGGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPISALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTCCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(4744..5757)
                     /gene="oppD"
                     /locus_tag="JNC24_002869"
     CDS             complement(4744..5757)
                     /gene="oppD"
                     /locus_tag="JNC24_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppD"
                     /protein_id="HAY9159566.1"
                     /translation="MSVIETATVPLAQQQADVLLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPERELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            complement(5769..6677)
                     /gene="oppC"
                     /locus_tag="JNC24_002870"
     CDS             complement(5769..6677)
                     /gene="oppC"
                     /locus_tag="JNC24_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HAY9159567.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTSGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            complement(6692..7612)
                     /gene="oppB"
                     /locus_tag="JNC24_002871"
     CDS             complement(6692..7612)
                     /gene="oppB"
                     /locus_tag="JNC24_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HAY9159568.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIVALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            complement(7698..9329)
                     /gene="oppA"
                     /locus_tag="JNC24_002872"
     CDS             complement(7698..9329)
                     /gene="oppA"
                     /locus_tag="JNC24_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HAY9159569.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDSTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLLYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            9613..9909
                     /locus_tag="JNC24_002873"
     CDS             9613..9909
                     /locus_tag="JNC24_002873"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9159570.1"
                     /translation="MRFLLANTFCKIVKQFSFIDRIRIYSLHPCLNKHLSIQSNRDWL
                     SMSIFSDYSSSSEMHNNLTIDYYLALSSTKGSGITNIISIILQQAQDYDVAKIT"
     gene            complement(10067..10714)
                     /gene="ychE"
                     /locus_tag="JNC24_002874"
     CDS             complement(10067..10714)
                     /gene="ychE"
                     /locus_tag="JNC24_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HAY9159571.1"
                     /translation="MIQTFFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            11192..13867
                     /gene="adhE"
                     /locus_tag="JNC24_002875"
                     /gene_synonym="adhC"
     CDS             11192..13867
                     /gene="adhE"
                     /locus_tag="JNC24_002875"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HAY9159572.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
BASE COUNT         3659 a         3437 c         3230 g         3579 t
ORIGIN      
        1 cttatagccg gtcagtgcat tcactaccac aagcagataa taactttatc tgtatttctt
       61 tatatgatgt gagcatattc aggaaaaatg acctttcctg aggtacagtt gctcgctcaa
      121 atcctttgat tactgcggat aaagtcatct actattgttt aacacattca cgcgctgcat
      181 gtatgttcag aaaatgtgca cgctaagata attaaacaaa attgtggtta cagaggtatc
      241 agtgagtcac tgggctacat tcaaacaaac agcaacaaat ttatgggtga cccttcggca
      301 cgacattctc gcgctggccg tctttttaaa tggattgctt atttttaaaa caatctatgg
      361 tatgtcggtc aatttgcttg atattttcca tatcaaagca ttttcagagt tggatctctc
      421 cttgctggca aacgccccac tatttatgct cggcgtcttt cttgtcctga actccattgg
      481 cttactgttc cgggcaaagc tcgcatgggc aatcagtatc attttgttgt tgatagcgct
      541 aatttacacc ctgcattttt atccctggct gaaatttagt attggatttt gcatttttac
      601 gctggtgttt ttgctgatac tgcgcaaaga cttctcccac agtagcgccg cagccgggac
      661 aatttttgca tttattagtt tcacgacatt actgttttac tcaacctacg gtgcgcttta
      721 tttaagcgaa ggttttaatc cgcgaataga aagtttgatg accgcgttct atttttcgat
      781 agaaaccatg tcaaccgtcg gctatggcga tattgtccct gtttctgaat cagcacgatt
      841 gttcactatt tcgatcatta tttccggcat taccgttttt gccacatcta tgacctcaat
      901 ttttggtccg cttatccgcg ggggattcaa caaacttgta aaaggaaaca atcatacaat
      961 gcatcgtaaa gatcatttta ttgtttgcgg acattcgatt ctcgccatca atacgatcct
     1021 gcaactgaat caacgcggac aaaacgtaac ggttatcagc aacttgcctg aagatgatat
     1081 caagcaactt gagcaacgct taggcgataa cgctgatgtt atccccggtg acagtaatga
     1141 cagttcagta ttaaagaaag cgggaatcga tcgttgccgg gccattctgg cgctgagtga
     1201 taacgatgca gataacgcgt ttgttgtact ctcggcaaaa gatatgagca gtgatgtcaa
     1261 aacagttctc gccgtcagtg atagcaaaaa cctgaataag attaagatgg tacatccgga
     1321 tatcattctc tcaccgcaac tgtttggcag cgaaattctg gcgcgagttt taaatggtga
     1381 agagattaat aatgatatgc tcgtttcaat gttgttgaac tccggtcatg gcattttcag
     1441 cgataacgat gaacaagaaa cgaaagctga cagtaaagaa tcagtgcaaa aatagagttt
     1501 aaagacgaaa gagtaaactt attgggcacc gcaatggtgc ccagtatatt tagatattgt
     1561 agatcgcaaa caataacgag ttacgctgtt tgtttagaat gcacttcctg atgctgtgga
     1621 tacgcatatt tcggcgcgat tgcccctcaa gccaacgcga tttacgtcgg ctagcctgac
     1681 gctgcatgcg ccagcgcccc acttccgttc tactccgctt catgttaact actctatgca
     1741 ataacagaat ggtcattata cccccacccc cgacgctgac cagtgctttt cccgcgtttt
     1801 tatttgccag atgaatccat atgcgtaaac tcataacaat gcgctttcaa aaggatttct
     1861 aatctatgac aaccgtttat acgttggtga gttggttggc cattctggga tactggttgc
     1921 tcattgcagg cgtaacttta cgcattctaa tgaaacgacg cgcagttccc tccgcgatgg
     1981 cctggctgtt gattatttac attctgccgt tagtcggaat tattgcctat cttgccgttg
     2041 gcgagctcca tttaggcaaa cgccgcgctg agcgcgccag agcgatgtgg ccttccaccg
     2101 caaaatggct taacgacctt aaagcctgta agcatatctt cgccgaagaa aatagcagtg
     2161 tcgctgcgcc attattcaag ctttgcgagc gtcgtcaggg gatcgctggg gtcaaaggga
     2221 atcagctaca actgatgacc gagtcagatg atgtgatgca ggcgttaatc cgcgacatcc
     2281 agctcgcgcg ccataatatt gagatggtgt tttatatctg gcagcccggc ggcatggcgg
     2341 accaggtggc tgaatcatta atggcggctg cacggcgtgg tattcattgc cgattgatgc
     2401 tcgactccgc cgggagtgtg gcttttttcc gcagcccgtg gcccgagcta atgcgtaatg
     2461 ccggtattga agtggtcgaa gccttaaagg tcaatctgat gcgtgtgttt ttacgccgta
     2521 tggacctgcg ccaacatcgc aagatgatca tgatcgataa ttacatcgcg tacaccggca
     2581 gcatgaatat ggtcgatcct cgctacttca aacaagatgc gggcgtaggg caatggattg
     2641 atctgatggc gcgtatggaa ggccccatcg ccaccgcgat ggggattatt tattcctgcg
     2701 actgggagat tgaaaccgga aaacgtattc tgccgccacc accagatgtc aatattatgc
     2761 cgtttgaaca ggccagcggt cacaccattc acacaattgc ttctggcccc ggctttccgg
     2821 aagatctcat tcaccaggca ttattgactg cggcttattc ggcgcgtgaa tatttgatca
     2881 tgacaacgcc ctactttgtg ccaagcgatg atttacttca tgcgatttgc acggcggcgc
     2941 agcgcggggt ggatgtcagt attatccttc cgcgaaaaaa tgactcgatg ctggtcggct
     3001 gggccagtcg cgcattcttt acggaactgc ttgctgctgg ggttaaaatt tatcagtttg
     3061 aaggcgggtt actgcatacc aagagcgtgc tggtcgatgg cgaactaagt ctggttggca
     3121 ccgttaacct tgatatgcgt agtctgtggc tgaatttcga gattaccctg gcaatcgacg
     3181 ataaaggttt tggtgcagac cttgccgccg ttcaggacga ttatatttcg cgttcacgtc
     3241 tgctcgatgc ccgtttatgg ctaaaacgtc cattatggca acgtgtcgcc gagcgactgt
     3301 tttacttctt cagcccgttg ctgtaaaacg tcgccaacag aggttaaaca ggtagtgact
     3361 atggatatgg atctaaacaa tcgcctgact gaagatgaaa cgcttgagca ggcctacgat
     3421 atttttctcg aactggctgc cgacaatctc gacccagctg atgttctgct gttcaatctt
     3481 cagtttgaag agcgcggcgg cgcggagtta ttcgatccgg cggaagactg gcaggaacat
     3541 gttgatttcg atttgaaccc ggactttttt gccgaagtgg tgattggtct ggcggacagt
     3601 gaggatggtg aaatcaacga tattttcgcc cgcattctgt tatgccgcga aaaagatcac
     3661 aaactttgcc atatcatctg gcgggaataa ataatcgctt tcggcgtgaa aaagggctga
     3721 caactgtcag cccttattgt gtttaaagcg gatcgacttt caggcaagaa acggcgtgtc
     3781 tgaaactccc ttccagcaca ggacgtgttt tggcgcactc cggaccggca atcgggcaac
     3841 aggtacggaa aacacaaccg gaaggcgggt tgatcggcga cggtaattcc ccttccagta
     3901 actggatggt tttgttcttc tccagatccg gatcaggtat ggggactgcc gacatcaatg
     3961 ccctggtgta aggatgtagt ggattgtggt agacctcatc ataggtcccc agttctaccg
     4021 catggccgag atacatcacc aacacacgat cggaaatgtg ttttaccacg gccaggtcat
     4081 gagcgataaa aattaatgac aatcccatct cacgttgcag ctgctggagc aggttgacca
     4141 cctgcgcctg aattgacacg tccagcgccg aaatcggctc atcgcagata atcagcttcg
     4201 gttcaagaat aagagcacga gcaatcccga tacgctggca ctgcccaccg gagaactcat
     4261 gcggatagcg gttaatcagg ttaggcaata acccgacttt cagcatcatc gccttcacgc
     4321 gctcgcgaac ttcctggcgt gacattttcg gatgataagt acgcagtggt tcagcgatga
     4381 tctcgccgat ggtcatacgc gggtttagcg atgccaacgg atcctggaaa atcatctgaa
     4441 tatcactgcg aacggcacgc cattcatcgg gcttcatgcc cagcaactct ttacctaacc
     4501 aggcaacatg accgccggtc gctttaacca ggccaatgat ggcgcgagca aaggtggact
     4561 taccgcatcc cgattcccct accacaccta atgtttcccc ttcatacagg cgaagcgtga
     4621 caccatcgac ggctttgaga gttttcggcg gttgccagaa ccactgtttg ccatctttga
     4681 tttcaaagtg cactttcaga tcggcgattt cgaggaggac ttttcttcct tcggttacag
     4741 cattcataac agttcctcca ccggtttaaa gcaagcacgc agacggccag gcgtaaactc
     4801 ttccagcggc ggtgcgctac tacaaatttc catcgcatgc ggacaacgtg gctggaacgg
     4861 gcaacctttc ggtaatcgca gcaggtttgg cggattacca gggatggtca acattgtttc
     4921 accttccgca tcgagacgcg gcaccgcgtt gagcaaaccg atagaataag gatgaacggg
     4981 ttgataaaag acatcgcgcg cgttgccata ttccatcgtg cgcccggcgt acattaccag
     5041 cactttgtcg cagatccccg ccaccacgcc aagatcgtgg gtaatcataa tgatggcggt
     5101 attaaattcc cgcttcagtt catttaatag cgtcatgatc tgcgcctgta cggtgacgtc
     5161 cagcgcagta gtgggttcat ccgcaatcag cagcttaggt cgacatagca atgccatcgc
     5221 aatcataact cgctgacgca tgccaccaga aaattcgtgc gggtacattt tcatgcgttt
     5281 acgtgcttcc ggcattttta ccgcatcgag catccgcacc gactcttcaa acgcctcagc
     5341 tttgctcatg ttcttatgca gcatcagcac ttccatcaac tgctcaccga cgcgcatata
     5401 gggattcaac gaagtcattg ggtcctgaaa aatcattgag atttgttcag cgcgcagttt
     5461 attgagttca cgctctggca aattgaggat ttcacgccca ttgaaggttg ccgatccgcc
     5521 aatacgtccg ttggcagcca acaggcccat caacgcaaat gcagtttgcg atttacccga
     5581 accagactca cctacaatgc ccagcgtttc tccggcacgt agagaaaaat tcaaatcatt
     5641 gaccgccgtg acatcgccgt ccggggtact aaaggtgaca cgcaaatctt tcacgttcag
     5701 cagtacgtca gcctgttgtt gtgcgagcgg cacagttgca gtttcaatta cgctcatggc
     5761 tgcactcctt aacgatcttt cgggtcgaga gcatcacgca agccatcgcc gataaagttg
     5821 aaacaaaaca gcgtcaccac gaggaatccc gctgggaaca acaataacca tggagagact
     5881 tccatcgagt tcgcgccatc actcagcaat gcaccccagc tgcttaacgg ctcttgcgta
     5941 cccaacccca ggaagctaag gaaagattca aagaggatca tgctgggcac cagaagtgat
     6001 gcgtagacca ccaccacacc gagtacattc ggcacaatgt ggcgaataac aatgcccgat
     6061 gtcgatacac cgccaacttg tgccgcctca ataaactctt tgcgcttcag actcagggtt
     6121 tgtccacgca caatacgagc catatccagc caggaaacca tgccaatcgc cacgaaaatc
     6181 agcaggatgt tttgaccgaa aaaggtcaca agcaaaatga cgaagaacat gaatgggaag
     6241 gagttgagga tttccagcag acgcatcatt accgaatcca ctttaccgcc cagataaccg
     6301 gaaagcgaac cgtaaagtgt ccccacgacc actgccacca gtgccgcagc aacaccgacc
     6361 atgagtgaga tacgcccgcc aatcgcaacg cgcacaagca ggtcgcgacc ggatgagtca
     6421 gtaccaaagt agtgaccgga ctccatatcc ggggcgctgg acatcatcgc ccagtcagta
     6481 tcgtcatagg caaactgcga aagcatcggt gccaggatta caaataacgc gatcagcacc
     6541 agcactatca gactggcaac cgccgcacgg ttatgcataa aacgtcgacg tgcgtcctgc
     6601 cacaagctgc gcccttcgac ctccagcttt tcactgaaat tttccagcgt ctcgctgttt
     6661 ttcttactta acatcatcgc gagctccagt atcagtaacg gattttcggg tcgataaccg
     6721 catatagcac atcgacaatg gcattaaaca aaatggttaa agcaccaacc aggatggtca
     6781 ggcttaacac taaggaatag tcacggttca atgcgccatt aacgaacagt tgtccaatcc
     6841 ccggcaaacc ataaatggtt tcgataacca tagaaccggt aataatgccg acaaatgcgg
     6901 ggcccatata agagagcacg ggtaacagag caggttttaa tgcgtggcgt aaaatgatcc
     6961 gccgcatagg taaccctttc gcccgggcag tacgaataaa gttggagtgt aatacttcaa
     7021 tcatagagcc tcgggtaata cgcgcaatac tggcgatata agcgagtgac aacgccacca
     7081 tcggcaatat catgaattta agcgccccac cattccagcc accgcccggc agccaatgca
     7141 aaatgatcgc aaatatcatg actaataatg gcgcaaccac aaaactgggg ataacaaccc
     7201 cggtcattgc cagccccatc acggtatagt cccatttggt gttttgtttt aatgcgacaa
     7261 taacgccagc actaacaccc agtattaccg caaggaaaaa tgcggcggct cccagtttgg
     7321 cagaaacggg aaaactggat gcaaccaggt cattgaccga ataatcttta tatttaaacg
     7381 atggaccgaa atcaccgtgc gccagttgtt tcaggtagct gaaatactgt gtcatgatcg
     7441 gatcattaag atgatatttc gcttcgatat tggccatcac ttccggcggt aaagtacgtt
     7501 cgccggtaaa agggcttccc ggcgcgaggc gcatcataaa gaacgaaata gtaataagaa
     7561 taaatagcgt cggaatcgct tccagacagc gacgtagaat aaattttaac attgccctac
     7621 cttctggcct gtgccttctg taatgcgata aaaatcagac accgtggagc aggacactcc
     7681 tgccccacgt attgccatta gtgcttcaca atgtacatat tccgggtgta ggtattatcc
     7741 agcggatctt tgccggtata gccaccaacc cacggtttca ccagacgcgc attcacgtag
     7801 taataaacag gaacaatggc cgaatcctta tccagctgtt gttctgcttt agtgtacaga
     7861 gctgtgcgct gcgcctcgtc agtcactttc agcgtttccg ccataatgct gtcaaaggcc
     7921 gggctcttat aatgcgcggt attcatcgag ctgttcgaca gcatggtgtt caggaaggaa
     7981 gttggttcgt tgtagtctgc acaccagcct gcacgggcca catcaaaagt gccctggtga
     8041 cgggtgtcga ggaacgtttt ccactcctgg ttaaccagtt tgacgtttac accaatgttt
     8101 ttcttccaca atgaagaggc agcaatcgcc agctttttat gcagatcaga ggtgttatac
     8161 agcaggttga tggtcaacgg tttgtctgcg gtataacccg cttcagccag cagttttttc
     8221 gcttcttcgt tacgtttttc ctggctccag ccaaaccatt ctggctgagt caattttgcg
     8281 ccatcagtat acggtggagt gtaaccatag gcgggcatgt cgccctgcgc tttcacttta
     8341 ttaacaatga tatcgcggtc catacctagt ttcagtgcgg tacgcacacg cacatcgttg
     8401 aatggcggtt tctggttgtt aatttcgtaa tagtaagtgc acaggtatgg atcaacgtga
     8461 acttcgtccg ggatctcttt tttcagcttc tggaacaatt cgatcggcat gttgttatac
     8521 gtcatgtcga tttcaccact acggtagcgg ttgacatcgg taacttcaga agcaataggc
     8581 aaataggtta cctggttaat aacggttttc gcgttgttcc agtaggtcgg gctgcgttca
     8641 agaacgattc gttcgtttac gacccaatct tttaaggtat aggcaccgtt ggtgacgata
     8701 ttaccaggct gggtccattt ttcgccgaat ttctcgatag cggcttttgg caccggtgaa
     8761 gttgatgggt gaacaagtaa tttatagaag tacggaacgg gttcacttaa ggtgacttct
     8821 aatgtgtgat catcaatagc tttcacgccg agatcggtaa tcggtttttt cccttcaaga
     8881 atttcatcaa taccggcgat atgcccatat tgcagataac tggcatacgg agaagcagtg
     8941 ttcggatcaa cagaacgttg ccagctatac acaaagtctt gtgctgtgac tggcgtgcta
     9001 tcagaccatt tcgcatcttt acgcaaatgg aaggtccaga ctttcgcgtc tttattatcc
     9061 caggattcag cgacgccagg tgctggatga ccgtcaagat cgctgaccag taagccttca
     9121 aacaggtctc ggctgatatt agactccgga acaccttcaa ttttgtgcgg atctaatgac
     9181 tgaacttctg aaccattgtt acgtaccagt gtttgttttt ccgccagtgt gacgcctgcg
     9241 ggtacatcag ctgccagcgc gacattccct gccattagcg cagccagaac gccagctgct
     9301 actaaacttc tcttggtgat gttggtcatt gttttttgga ctccctcatt ataattactg
     9361 gtattaacca gcatgtgtaa tcccggattg ggaccctgca caggctcgga gactttctgc
     9421 tgtcaggtta ctttatcgtt acgtattact atcaccgact ttatttattc tggtgattca
     9481 gacgatcacc ttatgtcgat tcgtactgtg tctcccctgt cgagacacat tattctttcg
     9541 cgacgctctt taattgttaa ttattctcat taccaataaa gaatcgtctg gcggttgccc
     9601 ggaaagtacc aaatgcggtt cttactcgcc aatacatttt gcaaaattgt taagcaattc
     9661 tcttttattg accgtatcag aatctatagc ttgcatccat gccttaacaa acatctttca
     9721 attcaatcaa atagagattg gctaagcatg tctatctttt ctgattacag ttccagtagt
     9781 gaaatgcaca ataatttaac aattgattat tatttagcac tttcctctac caaaggcagt
     9841 ggaattacta atatcatttc aattattctt cagcaagccc aggattatga tgtggcgaaa
     9901 ataacatgat ggtgaggaga agcagttaag gaaatcctta taactaactg taatttgaga
     9961 agaatatttt tacctattcc ttttgctttt gataactgcg cgggaacaaa gtaaaaataa
    10021 tgacgaagta aacggagcat ggcagctccg tttcatttga aaggaattaa ttaagcaggc
    10081 cggggaaaat ccccttaata ccagtaacga taaattcaat ccccaatgcc atcagcaata
    10141 gccccataat acgcgtaatc acgttgatgc cggtctggcg taaaacccgt accagccacg
    10201 gtgccatgcg gaacaatccc cagcaacata aagcgaacaa tgcaatagcc acaaagaaac
    10261 caaacagata gctaatgctg tgataacgcg taccccagac gatggtagaa ctgatcgccc
    10321 ctggccccgc catcaacggc aacgccaatg gcaccacacc aatgctttca cgtaccgcgg
    10381 tttctgattt ttcttgcttg ttctgtttat cctcgccaag cttgccgctg atcatcgaca
    10441 tcgctatcgt caccaccagg atgccccctg cgatacggaa cgaatcaatt gatataccaa
    10501 aaagttgcag aatcgtgtcg ccgagaaaaa gcgagatcca caagataatg gccacagaca
    10561 ggttggctgt aaggttagtt ttgtttcgcg ctgccgctgt ctgataactg gtcatgctga
    10621 taaagacggg aataatccct accgggttga ccagcgcaaa taacccgatg aaaaatttga
    10681 agtaaacggg aaaatcaaaa aaggtctgaa tcacggttag ctccgaagca aaagccggat
    10741 aatgttagcc ataaattaag gttgaaaaga cgcgctgaca atacgccttt tgacagcatt
    10801 tttcacctcc taactactta aaattgctat cattcgttat tgttatctag ttgtgcaaaa
    10861 catgctaatg tagccaccaa atcatactac aatttattaa ctgttagcta taatggcgaa
    10921 aagcgatgct gaaaggtgtc agcttagcaa aaatttgatt tggatcacgt aatcagtacc
    10981 cagaagtgag taatcttgct tacgccacct agaagtgacg cattagagat aataactcta
    11041 atgtttaaac tcttttagta aatcacagtg agtgtgagcg cgagtaagct tttgattttc
    11101 ataggttaag caaatcatca ccgcactgac tatactctcg tattcgagca gatgatttac
    11161 taaaaaagtt taacattatc aggagagcat tatggctgtt actaatgtcg ctgaacttaa
    11221 cgcactcgta gagcgtgtaa aaaaagccca gcgtgaatat gccagtttca ctcaagagca
    11281 agtagacaaa atcttccgcg ccgccgctct ggctgctgca gatgctcgaa tcccactcgc
    11341 gaaaatggcc gttgccgaat ccggcatggg tatcgtcgaa gataaagtga tcaaaaacca
    11401 ctttgcttct gaatatatct acaacgccta taaagatgaa aaaacctgtg gtgttctgtc
    11461 tgaagacgac acttttggta ccatcactat cgctgaacca atcggtatta tttgcggtat
    11521 cgttccgacc actaacccga cttcaactgc tatcttcaaa tcgctgatca gtctgaagac
    11581 ccgtaacgcc attatcttct ccccgcaccc gcgtgcaaaa gatgccacca acaaagcggc
    11641 tgatatcgtt ctgcaggctg ctatcgctgc cggtgctccg aaagatctga tcggctggat
    11701 cgatcaacct tctgttgaac tgtctaacgc actgatgcac cacccagaca tcaacctgat
    11761 cctcgcgact ggtggtccgg gcatggttaa agccgcatac agctccggta aaccagctat
    11821 cggtgtaggc gcgggcaaca ctccagttgt tatcgatgaa actgctgata tcaaacgtgc
    11881 agttgcatct gtactgatgt ccaaaacctt cgacaacggc gtaatctgtg cttctgaaca
    11941 gtctgttgtt gttgttgact ctgtttatga cgctgtacgt gaacgttttg caacccacgg
    12001 cggctatctg ttgcagggta aagagctgaa agctgttcag gatgttatcc tgaaaaacgg
    12061 tgcgctgaac gcggctatcg ttggtcagcc agcctataaa attgctgaac tggcaggctt
    12121 ctctgtacca gaaaacacca agattctgat cggtgaagtg accgttgttg atgaaagcga
    12181 accgttcgca catgaaaaac tgtccccgac tctggcaatg taccgcgcta aagatttcga
    12241 agacgcggta gaaaaagcag agaaactggt tgctatgggc ggtatcggtc atacctcttg
    12301 cctgtacact gaccaggata accaaccggc tcgcgtttct tacttcggtc agaaaatgaa
    12361 aacggctcgt atcctgatta acaccccagc gtctcagggt ggtatcggtg acctgtataa
    12421 cttcaaactc gcaccttccc tgactctggg ttgtggttcc tgggggggta actccatctc
    12481 tgaaaacgtt ggtccgaaac acctgatcaa caagaaaacc gttgctaagc gagctgaaaa
    12541 catgttgtgg cacaaacttc cgaaatctat ctacttccgc cgtggctccc tgccaatcgc
    12601 gctggatgaa gtgattactg atggccacaa acgtgcgctc atcgtgactg accgcttcct
    12661 gttcaacaat ggttatgctg atcagatcac ttccgtactg aaagcagcag gcgttgaaac
    12721 tgaagtcttc ttcgaagtag aagcggaccc gaccctgagc atcgttcgta aaggtgcaga
    12781 actggcaaac tccttcaaac cagacgtgat tatcgcgctg ggtggtggtt ccccgatgga
    12841 cgccgcgaag attatgtggg ttatgtacga acatccggaa actcacttcg aagagctggc
    12901 gctgcgcttt atggatatcc gtaaacgtat ctacaagttc ccgaaaatgg gcgtgaaagc
    12961 gaaaatgatc gctgtcacca ccacttctgg tacaggttct gaagtcactc cgtttgcggt
    13021 tgtaactgac gacgctactg gtcagaaata tccgctggca gactatgcgc tgactccgga
    13081 tatggcgatt gtcgacgcca acctggttat ggacatgccg aagtccctgt gtgctttcgg
    13141 tggtctggac gcagtaactc acgccatgga agcttatgtt tctgtactgg catctgagtt
    13201 ctctgatggt caggctctgc aggcactgaa actgctgaaa gaatatctgc cagcgtccta
    13261 ccacgaaggg tctaaaaatc cggtagcgcg tgaacgtgtt cacagtgcag caactatcgc
    13321 gggtatcgca tttgcgaacg ccttcctggg tgtatgtcac tcaatggcgc acaaactggg
    13381 ttcccagttc catattccgc acggtctggc aaacgccctg ctgatttgta acgttattcg
    13441 ctacaatgcg aacgacaacc cgaccaagca gactgcattc agccagtatg accgtccgca
    13501 ggctcgccgt cgttatgctg aaattgccga ccacttgggt ctgagcgcac cgggcgaccg
    13561 tactgctgct aagatcgaga aactgctggc atggctggaa acgctgaaag ctgaactggg
    13621 tattccgaaa tctatccgtg aagctggcgt tcaggaagca gacttcctgg cgaacgtgga
    13681 taaactgtct gaagatgcat tcgatgacca gtgcaccggc gctaacccgc gttacccgct
    13741 gatctccgag ctgaaacaga tcctgctgga tacctactac ggtcgtgatt atgtagaagg
    13801 tgaaactgca gcgaaaaaag aagccgctcc ggctaaagct gagaaaaaag cgaaaaaatc
    13861 cgcttaatca gtagcgctgt ctggcaatat aaacggcccc ttctg
//
LOCUS       DADMKF010000107        13794 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.109, whole genome shotgun sequence.
ACCESSION   DADMKF010000107 DADMKF010000000
VERSION     DADMKF010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13794
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.109"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(<1..147)
                     /locus_tag="JNC24_002876"
     CDS             complement(<1..147)
                     /locus_tag="JNC24_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HAY9159573.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKA"
     gene            389..1021
                     /locus_tag="JNC24_002877"
     CDS             389..1021
                     /locus_tag="JNC24_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005136294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HAY9159574.1"
                     /translation="MFQRLPCMDDFCRKINVNDKISPAGTVAIDQFVNRLGEAMQAVK
                     FGNPAERNDIAMGPLINAAALERVEQKVARAVEEGARVALGGKAVEGKGYYYPPTLLL
                     DVRQDMSIMHEETFGPVLPVVAFDTLEEAISMANDSDYGLTSSIYTQNLNVAMKAIKG
                     LKFGETYINRENFEAMQGFHAGWRKSGIGGADGKHGLHEYLQTQVVYLQS"
     gene            complement(1063..2064)
                     /gene="gap"
                     /locus_tag="JNC24_002878"
     CDS             complement(1063..2064)
                     /gene="gap"
                     /locus_tag="JNC24_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAY9159575.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYL
                     LKHDSNYGPFPWSVDFTEDSLIVDGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     AEKSQAHLDAGAKKVLISAPAGEMKTIVYNVNDDTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILGKKVTAEEVNNALKQATTNNESFGYTDEE
                     IVSSDIIGSHFGSVFDATQTEITAVGDLQLVKTVAWYDNEYGFVTQLIRTLEKFAKL"
     gene            2253..2783
                     /gene="cybB"
                     /locus_tag="JNC24_002879"
     CDS             2253..2783
                     /gene="cybB"
                     /locus_tag="JNC24_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HAY9159576.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVMYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            3028..3201
                     /locus_tag="JNC24_002880"
     CDS             3028..3201
                     /locus_tag="JNC24_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9159577.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            complement(3273..3422)
                     /gene="hokB"
                     /locus_tag="JNC24_002881"
     CDS             complement(3273..3422)
                     /gene="hokB"
                     /locus_tag="JNC24_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HAY9159578.1"
                     /translation="MKHNPLVVCLLIICITILTFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
     gene            3821..5461
                     /gene="trg"
                     /locus_tag="JNC24_002882"
     CDS             3821..5461
                     /gene="trg"
                     /locus_tag="JNC24_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HAY9159579.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASCIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEALDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMKDEPAGRNEIGRLNRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVDVFRLHKHSVSAEPRGAGEPVSFATV"
     gene            complement(5499..6422)
                     /locus_tag="JNC24_002883"
     CDS             complement(5499..6422)
                     /locus_tag="JNC24_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY9159580.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSK
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSASLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            6639..7982
                     /locus_tag="JNC24_002884"
     CDS             6639..7982
                     /locus_tag="JNC24_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAY9159581.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQQGGFNETQAQEFVQEALETFRWHQSATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVVAQPITYEDFLPVS
                     AAGIFQSNLGNETQTRSHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            8207..9862
                     /locus_tag="JNC24_002885"
     CDS             8207..9862
                     /locus_tag="JNC24_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein"
                     /protein_id="HAY9159582.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYALKDIKQLGIAP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            10002..10226
                     /locus_tag="JNC24_002886"
     CDS             10002..10226
                     /locus_tag="JNC24_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HAY9159583.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            10289..10825
                     /gene="rimL"
                     /locus_tag="JNC24_002887"
     CDS             10289..10825
                     /gene="rimL"
                     /locus_tag="JNC24_002887"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HAY9159584.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(10820..11800)
                     /locus_tag="JNC24_002888"
     CDS             complement(10820..11800)
                     /locus_tag="JNC24_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LbetaH domain-containing protein"
                     /protein_id="HAY9159585.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKSVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTIRDSLVYGQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVVRYAFIEHRAEVFDFASVEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ISGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            11924..12916
                     /gene="tehA"
                     /locus_tag="JNC24_002889"
     CDS             11924..12916
                     /gene="tehA"
                     /locus_tag="JNC24_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HAY9159586.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCLFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSENGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            12913..13506
                     /gene="tehB"
                     /locus_tag="JNC24_002890"
     CDS             12913..13506
                     /gene="tehB"
                     /locus_tag="JNC24_002890"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HAY9159587.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDRQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFPFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
BASE COUNT         3540 a         3197 c         3482 g         3575 t
ORIGIN      
        1 tgccttacgg gcatcctcgg cctgaccatc gggtatacgg gaaatgacag cctctgtagc
       61 agggtttacc acatcaatcc atgcgtctcc acgccaggta acaaactgtc catcgatata
      121 cataggatgt tgaacgggta ctgacatggg cgactcctgt gatttatatg tttgttttcg
      181 gtgaatatat tgttaattaa catcaatcat cagagagacg gcatcgggaa aggacaattc
      241 tgtgaagggc ttcataaaaa caagtgtaaa aggaataaaa ggcagaaata aaagtcagga
      301 gtcatgcccg tttgcaaaca agtggttcac aaacgggcaa tttttaacgt aacgagcgac
      361 tttccatcgc ctcgatgagc accgcatcat gtttcagcgt ttgccatgca tggatgactt
      421 ctgccggaaa atcaacgtga acgataaaat ctcgcccgcg gggaccgtag ccattgatca
      481 gttcgtcaat cggctgggtg aagcgatgca ggcggttaaa tttggtaacc ccgctgaacg
      541 caacgacatt gcgatggggc cgttgattaa cgccgcggcg ctggaaaggg ttgagcaaaa
      601 agtggcgcgc gcagtagaag aaggggcgag agtggcgttg ggtggcaaag cggtagaggg
      661 gaaaggatat tattatccgc cgacattgct gctggatgtt cgccaggata tgtcgattat
      721 gcatgaggaa acctttggcc cggttctgcc ggtggtcgca tttgacacgc tggaagaggc
      781 tatctcaatg gctaatgaca gtgattacgg cctgacctca tcaatctata cccaaaatct
      841 gaacgtcgcg atgaaagcca ttaaagggct gaagtttggt gaaacttaca tcaaccgtga
      901 aaacttcgaa gctatgcaag gcttccacgc cggatggcgt aaatccggta ttggcggcgc
      961 agatggtaaa catggcctgc atgaatatct gcagacccag gtggtttatt tacagtcgta
     1021 atgagtaaaa gaggcggagg ttttttcctc cgcctgtgcg cgtcagagtt tagcgaattt
     1081 ttcgagggtg cgaataagct gcgtgacgaa gccatattcg ttatcgtacc aggcgaccgt
     1141 tttcaccagt tgtaaatcgc ccacggcggt aatttccgtt tgcgtggcat caaacaccga
     1201 accgaaatgg ctgccaatga tatcggaaga gactatttct tcatcggtat aaccaaatga
     1261 ctcgttattg gtggttgctt gtttaagtgc gttattcacc tcttcggcag tcactttttt
     1321 tccgagaatc gataccagtt cagtgaccga acctgttttc accggcacgc gttgcgcatg
     1381 acctttcagt ttgccgctca gttccgggat caccagacca atggcttttg ccgcccccgt
     1441 agtgtgggga atgatatttt ctgccgctgc gcgtgaagca cgtaaatctt taccacgtgg
     1501 gccatccacc agtgactggg tgccagtata ggcatgaatg gtcgtcatcg tgccgacttc
     1561 tatcccgaaa ctgtcatgca aggctttggc catcggcgca agacagttag tggtgcatga
     1621 cgccacggaa acaatggtgt cgttgccatc cagagtgtcg tcattgacgt tataaacgat
     1681 agttttcatt tcaccggcag gggcggaaat caacaccttc ttcgcaccag catcaagatg
     1741 tgcctgcgat ttctcggcgg aggtataaaa gccagtacat tcgacaatga tttctgcacc
     1801 tttcgctttc cacggaatat ttttagcctc tttttcggcg taaaccgcga tacttttccc
     1861 atcaacgata agtgaatctt ccgtaaaatc aacgctccag gggaatggtc cgtagtttga
     1921 atcatgtttc agcaggtagg cgagaatttt tggggaagtg agatcattaa tagcgacaac
     1981 gtctatgttg cttttgactt caagtaatcg acgcaacacc agtcgaccga tacgaccaaa
     2041 accgttaata ccaactttac tcatggtttt ctcctgtcag gaacattcgg atgaaaattg
     2101 atcctttcca ggcttagacc aggattgtgg gatgggcaat ctccattctt acagtgaaac
     2161 gtaacgtact gaaaacgggt gaacaatatt taatgaaatt ttgagaaaag cccgttatgt
     2221 taacggaaaa ttatgttaaa gcaggaaatg ttatggaaaa taaatactca aggttacaaa
     2281 tcagcattca ctggctggtc tttttgctgg ttatcgcagc gtattgcgca atggagtttc
     2341 gtggtttctt cccacgtagc gatcggccac tcatcaacat gattcatgtt tcctgcggca
     2401 tctcaattct cgtgctgatg gtcgttcgtc ttttgttaag gctgaaatac ccaaccccgc
     2461 cgattatacc taagccaaaa ccgatgatga cgggactggc gcatttggga catttggtga
     2521 tgtatctcct gtttattgcg ctgccagtga ttggtttggt gatgatgtat aaccggggca
     2581 acccgtggtt tgcgtttggt ttgacgatgc cttacgcttc agaggccaat ttcgaacggg
     2641 ttgatagctt aaagtcgtgg catgagacgc tggcgaatct gggatatttt gtcatcgggt
     2701 tgcacgctgc ggctgcactg gcacaccact atttctggaa ggacaacaca cttctacgca
     2761 tgatgccgcg taaacgttcc tgaaggatat ttcaagaaaa tgcctgtact aaaaccgacc
     2821 cgtggtacag gcgaagaata cgggtctaca tcggaagcgc ctattatatt tatttgtatg
     2881 acaaataaaa ccataatcct tgcccatacg tccatctggc ttatttttaa tcaattcacc
     2941 cgatctttga tctcatcaac ggtatcaaaa taaaccagcg tataacgttc aaatcgaaca
     3001 taagattcga taaaccatgg aggttatatg aaaaaactgg cacttatttt gtttatggga
     3061 acgcttgttt ccttttatgc cgatgccggg cgcaaaccct gttctggttc gaaagggggg
     3121 atctcacact gtacggcagg cggcaaattt gtctgtaatg atggttctat tagtgcatcg
     3181 aaaaaaacat gcactaactg aagtgtaaaa ggggtgccat gagaaaatga atctgctagt
     3241 cagatgcgcg gggaaaatcc ccgcgcttgc ccttacctgg acgtgcaggc catgagcgca
     3301 gcaacctcct tatcaccgtc ccggaaccgc agttcgtaga gcgtttgtcg ggtcaggagt
     3361 gtgaatgtca gaatcgtaat gcagataatg agcagacaca ctaccagagg gttgtgcttc
     3421 atagcctttc tccttgccgg atggcgggta agaggctaag atctgaattg ctaggttcat
     3481 tcgttggcct cggttgatag aaatatcggt cggggccttc gtctttctga ttcccggtta
     3541 gcctgaaaac agaaagtctc aggcacccgc aggcatccta tgaggtttcc ttagggacga
     3601 aaataatcac ttcacgaaat tgcgtgctgt tttccagaat ttttcgtcat tcgggttagc
     3661 cagtttagcc attcgttact ctcttcattc caatagcatt aattttctct gcaataattg
     3721 ttgtaaaaat gtgacgcaaa gaggtttttg gtcataagta attaccgtca agtgccgatg
     3781 actttctatc aggagtaaac ctggacgaga gacaacggta atgaatacaa ctccctcaca
     3841 gcgattaggt tttttgcatc acatcaggct ggttccgtta tttgcctgca ttcttggcgg
     3901 tatcttagtt ctattcgcat taagttctgc tctggctggc tatttcctct ggcaggccga
     3961 tcgcgatcag cgtgatgtta ctgcggagat tgagattcgg accgggttag cgaacagttc
     4021 agattttttg cgttcagccc ggatcaatat gattcaggcc ggggctgcga gttgtattgc
     4081 ggaaatggaa gcaatgaagc gaaatattgc gcaagccgaa tcggagatta aacagtcgca
     4141 gcaagggtat cgtgcttatc agaatcggcc ggtgaaaaca cctgctgatg aagccctcga
     4201 cactgaatta aatcaacgct ttcaggctta tatcacgggt atgcaaccta tgttgaaata
     4261 tgccaaaaat ggcatgtttg aagcgattat caatcatgaa agtgagcaga tccgaccgct
     4321 ggataatgct tataccgata ttttgaacaa agccgttaag atacgtagca ccagagccaa
     4381 ccaactggcg gaactggccc atcagcgcac ccgcttgggt gggatgttca tgattggcgc
     4441 gtttgtgctt gccctggtca tgacgctgat aacatttatg gtgctacgtc ggatcgtcat
     4501 tcgtccactg caacatgccg cacaacggat tgaaaaaatc gctagtggcg atctgacgat
     4561 gaaggatgaa ccggcgggtc gtaatgaaat cggtcgctta aatcgtcatt tacagcaaat
     4621 gcagcattca ctggggatga cagtagggac tgttcgacag ggtgcggaag agatttatcg
     4681 tggcaccagc gaaatttcag ctggcaatgc ggacctgtca tctcgcaccg aagaacaagc
     4741 ggcggctatc gaacaaactg ccgccagcat ggagcaactc actgcgacgg tgaaacagaa
     4801 tgcggataac gcgcatcatg ccagcaaact ggcgcaagag gcttctatta aagccagcga
     4861 tggcgggcag acggtttccg gtgtagtaaa aacgatgggc gctatctcca cgagttcgaa
     4921 gaaaatttct gagatcaccg ccgtcatcaa cagtattgct ttccagacga atattctggc
     4981 actgaatgct gccgttgaag ccgcgcgagc gggtgagcaa gggcgtggat ttgccgttgt
     5041 cgccagcgaa gtacggacac tcgcaagtcg cagcgctcag gcggcgaaag agattgaagg
     5101 cttgatcagt gaatcagtca ggttaattga cctggggtcg gatgaggtgg caacggccgg
     5161 gaaaaccatg agcactattg ttgatgccgt cgcgagtgtc acacatatca tgcaggaaat
     5221 cgccgccgcc tcggatgaac aaagtagagg cataacgcag gttagccagg cgatttctga
     5281 aatggataag gtgacgcaac agaatgcttc tctggtagaa gaggcctcag cggcggcggt
     5341 gtcccttgaa gaacaggcgg cacgattaac tgaggcggtg gatgtattcc gtctgcacaa
     5401 acattctgtg tcggcagaac ctcgcggagc gggtgaacca gttagtttcg ctacggtgtg
     5461 aaaatgttca aggagggatc gacagatccc ttcacctttc agaacggcat tgattttcga
     5521 atagcgttaa tcatcaactg gcaaccagaa gagaacgtcg catctacgcg ggtcagtatt
     5581 ccaatcggtt cgcctgcacc atgtcccgga acaggcaggg ccaccagcgt ggcatgacgc
     5641 aggtcgtctt ttacagcgcc agaagggaca aaccacacgt aatcgtattc aaccgtaagt
     5701 tgacgagata gcgaagcaga cagcgtttcg atacaacccg aaggaatttt acagccctgg
     5761 ctctgcacta atgcatctga atgctggcgt ggcgcagtgc cttctggtga tacaacgacc
     5821 ggccattcca gcacccggct tagcgttacg ttctcctgaa gtagcgggtg attagggcgg
     5881 acaaccagct tcagcgattc aagaaacagc agttcgtaat taagcccggt catcagttca
     5941 ggatctgaca tccgaccaat gccgatatcg atttccccgg tttttaaacc cgccagaatc
     6001 atagggttac tcattgtcgc aacttgcaag gtcgtctctt tttgttgctg atgaaactga
     6061 cctataaccg aaggtaatat ccccagtgcc gcagtaggta gtgcaccaac cctgacgaca
     6121 tcattattaa gaccttcttt acgatgaagc gactgtccgg cagtgttgat ggcgtcaaga
     6181 actctgactg catgcgttaa aaattgttcg ccgggtaagg taagttgcgc cccctgacga
     6241 ccacgctcaa acaagcgagc gcccgtcagc tgctccagtt cattcaatgt cttagagagc
     6301 gcaggttgac tcaaattaag ggtttcagcc gcgcgcccca aagttccttg ttgtgcgaca
     6361 gctacgaatg tatgaaggtg gcgcaaacgg atgcgctgac taaacagact atttttttcc
     6421 ataagcgatg ttaaaaacga agcggtgtcg ctgacaagtg aagttgtttg attatgataa
     6481 cttgattgca aaatattatt aacaattaaa gcaattatgt tacagcaaaa tggataatat
     6541 tgatgttttc gcggcgagat cacagtttgt aaattcttcc cgccagagtg aatgcggtta
     6601 cctacactcc agattactga ccactggagg cagacactat ggcgaacagc atcacggcgg
     6661 atgagattcg ggaacagttt tcgcaggcaa tgtcagccat gtaccagcaa gaagttccgc
     6721 aatatggcac gctgctggaa ctggtagctg atgtgaatct ggctgtgctg gaaaacaatc
     6781 ctcaactgca cgaaaaaatg gtaaatgcag acgagctggc gcgactgaat gttgaacgtc
     6841 atggggcgat tcgcgttggg actgcacaag agcttgctac tcttcggcgg atgtttgcca
     6901 ttatggggat gtacccggtg agctattacg atctctcgca ggcaggggtg ccggtacatt
     6961 cgacagcatt tcggcccatt gatgatgctt ctctggcgcg taatcccttc cgcgttttta
     7021 cctccttact ccgccttgag cttatcgaga acgaaatttt gcgccagaaa gcggcggaga
     7081 ttctgcgtca gcgcgatatc ttcaccccac gttgtcgaca actgttagag gaatatgagc
     7141 agcagggcgg ttttaacgaa acacaggcac aggagtttgt gcaggaagcc ctggaaacgt
     7201 ttcgctggca ccagtcagca acggtagatg aagaaaccta tcgcgccttg cacaacgaac
     7261 atcggttgat tgctgatgtg gtctgttttc ctggatgcca tatcaaccac ctgacgccac
     7321 gtacgctgga tattgaccgg gtgcagtcga tgatgcctga atgcggaatt gaacccaaaa
     7381 ttctcatcga ggggccgccg cgccgcgagg taccgatttt actacgccag accagcttta
     7441 aagcactgga agagacggtg ttgtttgcgg ggcagaaaca gggcacgcat accgcgcgct
     7501 ttggtgaaat tgagcagcgt ggcgtggcat taacgccgaa agggcgacaa ctgtatgatg
     7561 atcttctgcg taacgctgga accgggcagg ataatctcac tcaccaaatg catttacagg
     7621 aaaccttccg cacttttcct gacagtgagt ttttaatgcg tcagcaaggg ttggcatggt
     7681 tccggtaccg tctgacgcct tcgggtgagg cgcatcgtca ggcgattcat cctggagacg
     7741 atccacagcc cttaattgaa cgtggttggg tagtggcgca acccatcacc tatgaagatt
     7801 tcttgcccgt tagcgcggcg gggatcttcc agtcaaatct gggtaatgaa acgcagacac
     7861 gcagtcacgg taatgccagt cgcgaagcat ttgagcaggc gttgggttgt ccggttttgg
     7921 atgagttcca gctttaccag gaagcggaag aacgcagtaa acgtcgctgt ggtttgcttt
     7981 aaattctgac catccgcctt tgcaaaaatt tgcctgattt tacaaacgaa tcaggctcat
     8041 cccatcgaca taaaaaaaat gccgatttat gcatattctc tcagttcaac aattggatta
     8101 ttaataaata ttgtctagag tgagcggtca taaataagca ctttcttgcc gctgaaaacg
     8161 accagcgcgg gaccattcac aacaccagaa ggactcactt tcaggtatgg atcgtagacg
     8221 atttattaaa ggttcaatgg ctatggccgc cgtgtgcggt accagcggca ttgcttctct
     8281 tttttctcag gcggcattcg cggcagattc tgatattgcc gacgggcaaa cccagcgttt
     8341 tgacttctcc attctacagt caatggcgca cgacttagcg caaacagcgt ggcgtggtgc
     8401 gcctcgtccg ttacctgaca cgctggcgac aatgacgccg caggcttata acagtattca
     8461 atacgacgcc gaaaaatcgc tctggcataa cgttgagaac cgtcaactgg acgctcagtt
     8521 cttccatatg ggaatgggat tccgtcgccg cgttcgtatg ttttctgtag atccagcaac
     8581 acatctggcg cgtgaaattc actttcgccc ggagttgttc aaatacaacg atgcaggtgt
     8641 tgataccaaa caattagaag ggcaaagcga tctcggcttt gccggttttc gcgtgtttaa
     8701 agcccccgaa ctggcgcgcc gtgatgtagt atcatttctc ggcgcgagtt atttccgcgc
     8761 cgttgatgat acatatcaat acggtttgtc ggcccgcggc ctggcgatcg acacttacac
     8821 cgacagtaaa gaagagttcc ccgactttac cgccttctgg tttgatacgg taaaaccggg
     8881 ggcaactacc tttaccgttt atgcgttgct cgatagcgcc agtattactg gtgcctataa
     8941 gttcactatc cattgtgaga aaagtcaggt gattatggat gtggaaaatc acctgtatgc
     9001 gctcaaagac attaaacagc tgggcattgc gccgatgacc agtatgttca gctgcggtac
     9061 taatgaacgt cggatgtgcg acaccattca tccgcaaatt catgactctg atcgtctgtc
     9121 catgtggcgg ggcaacggcg agtggatttg ccgtccgctg aacaatccgc aaaaattgca
     9181 gttcaatgct tacaccgaca acaacccgaa agggtttggt ttattgcaac tggatcgtga
     9241 cttctcccat tatcaggaca ttatgggctg gtataacaaa cgcccaagtt tgtgggtgga
     9301 accgcgtaac aagtggggta agggcaccat cggcctgatg gaaatcccaa caacgggcga
     9361 aacgctggat aacattgtct gcttctggca gccagaaaaa gctgtaaaag caggtgatga
     9421 gtttgcattc cagtatcgtc tgtactggag tgcgcaaccg cctgttcatt gcccattagc
     9481 gcgcgttatg gcgacgcgta ccggcatggg cggtttcccg gaaggttggg cgccaggtga
     9541 acactatccc gaaaaatggg cgcgtcgttt tgccgtcgat ttcgttggtg gtgatctgaa
     9601 agctgccgcg ccaaaaggca ttgagccggt gattacgctt tccagtgggg aagcgaagca
     9661 aatcgaaatt ctctatattg aacccatcga tggttatcgt attcagtttg actggtatcc
     9721 gacttcggac tctactgatc cggtcgatat gcggatgtat ctacgttgtc agggggacgc
     9781 tatcagtgaa acatggctgt atcagtattt cccgccagcg ccggataaac gtcagtatgt
     9841 tgacgaccgc gtgatgagtt aatcgttttt tcttcggcac cttcttcggg aggtgccgtc
     9901 tggttaaaca cgatcccgct cgcatttttc cctaagttaa atgagtaatc tgatggtgtg
     9961 tatttcagat acaccttgtc agccactaac agggagtgcg tatgtttcca gaataccgag
    10021 atttaatatc ccgtctgaaa aacgaaaatc ctcgctttat gtccttgttc gataaacaca
    10081 ataaacttga tcatgaaatt gccagaaagg aaggttccga cggtcgaggg tacaatgcgg
    10141 aagtggtccg catgaaaaaa caaaagctac agttaaaaga tgagatgctc aaaatcctgc
    10201 agcaggagag cgtcaaagag gtgtaaactt tcctaagccg ccagccaggc ggctttttta
    10261 acaacagcat ggattgactg gagataagat gactgaaacg ataaaagtaa gcgaatcact
    10321 tgaattacat gctgttgcgg aaagtcacgt cacacctctt tatcagttaa tctgtaaaaa
    10381 taaaacctgg ttacagcagt cgctaaactg gccgcagttc gttcagagtg aagaggacac
    10441 gcgaaaaacg gtgcagggta atgtgatgtt gcatcaacgc ggctatgcca aaatgttcat
    10501 gattttcaaa gaagatgaac ttatcggcgt tatctcgttt aatcgtattg aaccactgaa
    10561 taaaactgct gaaatcggct actggctgga cgaatctcat caggggcagg ggatcatttc
    10621 tcaggcgctg caggcattga ttcatcatta cgctcagtct ggtgaactta gacgcttcgt
    10681 gatcaaatgt cgggtggaca atccgcaaag caaccaggtc gctttgcgca atggttttat
    10741 ccttgaaggt tgcctgaaac aggctgagtt cctgaatgat gcctatgatg atgtgaactt
    10801 atacgcgcgt attatcgatt cataataacc ctgccagcgg cgttcgcgta atgcgctcct
    10861 cgccgttaat gacttttgga ccacggacat gaaccgtgtc gccatcaaag gcttcaacga
    10921 cggcgtggtc ggttaactcc acatggtttt caattatcac cgcacctgaa atacggctct
    10981 caccttgtat tacaacgtgc tcgtcgagca gaatcggtcc accacgtacc acggcatttc
    11041 cgccaatcaa gacatgatgt tttaacacac aattaccttc cacaatggca tattccgcca
    11101 cctgcgaact gtaatgaatc gtcggaatgg catcttcttc tatgccagct ttcacctgcg
    11161 cgtgaccgta gacttttgcg caatcgcata gccagacgtt gttctcttca ttaccttcaa
    11221 cactggcaaa atcaaaaact tcggcgcgat gttcaataaa agcataccgg acgacggcat
    11281 cgccataaat ttgtgcctgg tggacaatac gcgaggcgct aacccttgcg cgatcataaa
    11341 tttggagcag gagttgatgg tcgggcgtta agccttgtgc ggcgacgatc atagaatgtt
    11401 gatcaattaa ggcgtgacca aatattctac attgcccgta aactaatgag tcacgaatgg
    11461 tgacactgtc acttatatat gcaccctggc tgatttcact attatcgatc cagacgttat
    11521 ccgttgcgta aacctctccc cacagcacgc tggtgccggt gatacgcgta ttaccagaaa
    11581 tcaccgttcc gccgaacgca atagcattct ggtcgtaaat ccagcaattt ccttcctgtg
    11641 ctaacaccgt ttcgcgatcg atccagccgc cagcggttcc tgctatcaca tcgttaaaat
    11701 cgctaatggc gataatctgc cgtaataaca cacttttttt agtgccatct tcctgataac
    11761 taaaggctcg ctgttcttca cttagacgat atttgcgcat agcgttttct cacaggtgac
    11821 ttactataac cgtagcaaat tctgcggctc tggctatgct cgagaaaatt ccataaaatg
    11881 catttcaaat atactttata aattaaacaa aatgagtaag aagatgcaga gcgataaagt
    11941 gctcaatttg ccggcaggct actttggtat tgtgttgggg acgataggga tgggatttgc
    12001 ctggcgctat gccagccagg tttggcaggt cagccactgg ttaggggatg ggctggtgat
    12061 tctggcgatg atcatctggg gattattaac tagcgcattt attacccgac tcatacgctt
    12121 tccgcatagc gtgctggcgg aagttcgcca tccagtgctg agcagttttg tgagtttgtt
    12181 tccggcaacg acgatgctgg tggcgattgg ttttgttccg tggtttcgcc cactggcggt
    12241 gtgcctgttc agttttggtg tcgtggttca gttggcttat gccgcctggc aaactgcggg
    12301 attatggcgc ggatctcacc ctgaagaagc taccacgcct ggactgtatc tgccgacagt
    12361 tgccaacaac tttatcagcg caatggcctg tggtgcgttg ggctacaccg acgccggtct
    12421 ggtgttttta ggcgcaggcg ttttctcatg gctaagtctt gaaccggtga tcttgcagcg
    12481 tctgcgcagt tcgggagaat tacccacggc actgcggaca tcactcggca ttcagctcgc
    12541 tcctgcgctg gtggcctgta gtgcctggtt gagcgtcaac ggcggcgagg gtgatacgct
    12601 ggcgaaaatg ctttttggtt atggactgct gcaactgctg tttatgctac gtctgatgcc
    12661 atggtatctc tcccagccat ttaatgcttc attctggagt ttctcgttcg gcgtatctgc
    12721 actggcaacc accggtttgc atctggggag tggcagcgaa aatggatttt tccatacgct
    12781 ggcggtaccg ctgtttatct ttaccaattt tattattgca atactgctca tccgtacttt
    12841 tgcgcttctg atgcagggaa aattgttagt cagaaccgag cgcgccgttt taatgaaagc
    12901 agaggacaaa gaatgatcat tcgtgacgaa aactatttta ctgataaata tgaattaacc
    12961 cgcacacact ctgaagtact ggaagcggtg aaagtggtta aaccgggtaa aacgctggat
    13021 ctgggctgtg gcaatggtcg taacagtctt tacctggcag ccaatggtta tgatgttgac
    13081 gcatgggata aaaatgccat gagtatcgcc aatgtagagc gcattaaatc cattgaaaat
    13141 ctggataatt tacacacccg agtcgttgat ctgaataacc tcacatttga tagacagtac
    13201 gattttattc tttcgactgt ggtactgatg ttccttgagg ctaaaaccat cccagggctg
    13261 attgccaata tgcaacgttg cactaaacct ggtggttaca acctgattgt ggcggcgatg
    13321 gataccgctg attatccatg taccgtcggc ttcccgtttg ccttcaaaga gggagaatta
    13381 cgtcgatatt acgaaggctg ggagagggtg aaatacaatg aagacgtcgg cgagctgcac
    13441 cgcaccgacg ccaacggtaa tcgtattaaa ctgcgtttcg ccacgatgct ggcacgtaaa
    13501 aaatgacccg ctaagaataa ggcgcgtgaa aattccccac gctgagatga tttactgttc
    13561 ttcttttcgg taagcatatt ttttatcgaa gggatgtgaa attaatcaca gtagtcgaag
    13621 ttttttagca gcttaactta ctgaaattta actactgatg attgacttag cccctttttc
    13681 ggcattgact atgtcgtctg aaaaggggct gaaaaattta ttttcaccaa cacttttttt
    13741 gccacaacac gaagcggcgc tttttgctat aacttaaaaa gtaatataat catc
//
LOCUS       DADMKF010000108        13778 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.110, whole genome shotgun sequence.
ACCESSION   DADMKF010000108 DADMKF010000000
VERSION     DADMKF010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13778
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.110"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(<1..730)
                     /gene="dsbG"
                     /locus_tag="JNC24_002891"
     CDS             complement(<1..730)
                     /gene="dsbG"
                     /locus_tag="JNC24_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HAY9159588.1"
                     /translation="MLKKILLLALLPAIAFAEELPSPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWILDGKKDAPVIVYVFADPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQQYEASGGKLKLNVPANVSTEQMKVLSDNEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNI"
     gene            1102..1665
                     /gene="ahpC"
                     /locus_tag="JNC24_002892"
     CDS             1102..1665
                     /gene="ahpC"
                     /locus_tag="JNC24_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HAY9159589.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            1836..3401
                     /gene="ahpF"
                     /locus_tag="JNC24_002893"
     CDS             1836..3401
                     /gene="ahpF"
                     /locus_tag="JNC24_002893"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HAY9159590.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGDIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(3522..3950)
                     /gene="uspG"
                     /locus_tag="JNC24_002894"
     CDS             complement(3522..3950)
                     /gene="uspG"
                     /locus_tag="JNC24_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HAY9159591.1"
                     /translation="MYKTIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVIR"
     gene            4171..5409
                     /locus_tag="JNC24_002895"
     CDS             4171..5409
                     /locus_tag="JNC24_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent formaldehyde
                     dehydrogenase"
                     /protein_id="HAY9159592.1"
                     /translation="MKALTYHGPHHVQVENVPDPGIEQADDIILRITATAICGSDLHL
                     YRGKIPQVKHGDILGHEFMGEVVETGKDVKNLQKGDRVVIPFVIACGDCFFCRMQQYA
                     ACENTNAGKGAALNKKQIPAPAALFGYSHLYGGVPGGQAEYVRVPKGNVGPFKVPPLL
                     SDDKALFLSDILPTAWQAAKNAQIQQGSSVAVYGAGPVGLLTIACARLLGAEQIFVVD
                     HHPYRLRFAADRYGAIPINFDEDSDPAQSIIEQTAGHRGVDAVIDAVGFEAKGSTTET
                     VLTNLKLEGSSGKALRQCIAAVRRGGIVSVPGVYAGFIHGFLFGDAFDKGLTFKMGQT
                     HVHAWLGELLPLIEKGLLKPEEIVTHYMPFEEAARGYEIFEKREEECRKVILVPGAQS
                     AEAAQKAVSGLVNAMPGGTI"
     gene            complement(5640..6050)
                     /gene="rnk"
                     /locus_tag="JNC24_002896"
     CDS             complement(5640..6050)
                     /gene="rnk"
                     /locus_tag="JNC24_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HAY9159593.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            complement(6280..7086)
                     /gene="rna"
                     /locus_tag="JNC24_002897"
     CDS             complement(6280..7086)
                     /gene="rna"
                     /locus_tag="JNC24_002897"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HAY9159594.1"
                     /translation="MKAFWRNAALLAVSLLPFSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEAHASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKNFVIDKVGY"
     gene            complement(7200..8663)
                     /gene="citT"
                     /locus_tag="JNC24_002898"
     CDS             complement(7200..8663)
                     /gene="citT"
                     /locus_tag="JNC24_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HAY9159595.1"
                     /translation="MSLAKDNIWKLLAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAM
                     IVGMILEPIPATAISFIAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSST
                     TVWLVFGAFIFALGYEVSGLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNT
                     ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVL
                     GLEFVSKIAGIQISWLQWFLCFLPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDE
                     LKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITR
                     YNSAWNTLVNLATLVVMANGLTRSGFIDWFAGTMSTHLEGFSPNATVIVLVLVFYFAH
                     YLFASLSAHTATMLSVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIY
                     GCGYVKSKDYWRLGAIFGVIYISMLLLVGWPILAMWN"
     gene            complement(8714..9592)
                     /gene="citG"
                     /locus_tag="JNC24_002899"
     CDS             complement(8714..9592)
                     /gene="citG"
                     /locus_tag="JNC24_002899"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HAY9159596.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEIAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSQLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMAINGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPADLDYLRQFDRECIERNLSP
                     GGSADLLILTWFLAQI"
     gene            complement(9567..10118)
                     /gene="citX"
                     /locus_tag="JNC24_002900"
     CDS             complement(9567..10118)
                     /gene="citX"
                     /locus_tag="JNC24_002900"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HAY9159597.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHVWLKRHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHGVTALRALAAKQGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGEILSRRDYSLPPRRCLLCEQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            complement(10122..11654)
                     /gene="citF"
                     /locus_tag="JNC24_002901"
     CDS             complement(10122..11654)
                     /gene="citF"
                     /locus_tag="JNC24_002901"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HAY9159598.1"
                     /translation="MTQKIEQSQRQERVAAWNRRAECDLAAFQNSPKQTYQAEKARDR
                     KLCANQEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVDRVGDAAKIGAGATRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDTQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            complement(11665..12573)
                     /gene="citE"
                     /locus_tag="JNC24_002902"
     CDS             complement(11665..12573)
                     /gene="citE"
                     /locus_tag="JNC24_002902"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HAY9159599.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            complement(12570..12866)
                     /gene="citD"
                     /locus_tag="JNC24_002903"
     CDS             complement(12570..12866)
                     /gene="citD"
                     /locus_tag="JNC24_002903"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HAY9159600.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILRARLEALLARASGIPALPWEDCQ"
     gene            complement(12881..>13778)
                     /gene="citC"
                     /locus_tag="JNC24_002904"
     CDS             complement(12881..>13778)
                     /gene="citC"
                     /locus_tag="JNC24_002904"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HAY9159601.1"
                     /translation="AGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTK
                     TEYEALFRQCGFSTLTSVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANP
                     FTNGHRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEY
                     IISRATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYN
                     QDMRYWLETPTISAPPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTL
                     HYLQNLLEHSRQDAAARQKTPA"
BASE COUNT         3379 a         3491 c         3670 g         3238 t
ORIGIN      
        1 tgatattaag cgttttctga tcgggcaacc ccacggcctg ttgcagcgta ttttccttac
       61 tcatgtaata gatagccggc gtgacatttg cccccagatc gtccatcagt ttctcattgt
      121 cacttaacac tttcatttgc tctgtactta cgtttgcagg cacgtttagc ttaagcttgc
      181 caccagaggc ttcatattgt tgccaggttt ttgcgggatc tttggaggca agaattgccg
      241 ctgctgtcgc cgggctttct ggcttgataa ccccaaccaa caatgttctt aattgcactt
      301 tgccagaatc tacccacggg cgcgcctgct gccagaactg tttacaatat gggcagaacg
      361 gatcggcgaa gacgtagaca atgaccggcg catctttttt accgtcgagg atccagtggg
      421 attgttccat ccgttgccac atttcgcgtc cggctggtgc gtaaatttct ttttcgataa
      481 gtgtgttact caggttttca cctttctcgt tgtacatgta accagagata gcgtgcttac
      541 catcgggagt caggtagatg gtgacgccca tatcctgata ctttccgaga taacctttca
      601 ttcctccggg ggcatcgaac gttttgatga ttgtaatgcc ctgtttttca atcgctttta
      661 ctggggaagg aagttcctct gcgaaggcga ttgcaggaag cagagccagt aaaagtatct
      721 tttttaacat taatttgtcc ttttcagtca gtgcaaaagt cgagtaaaag gcataaccta
      781 tcactgtcat aggtaagagc ttagatcagg tgattgccct ttgtttatga gggtgttgta
      841 atccatgtcg ttgttgcatt tgtaagggca acacctcagc ctgcaggcag gcgctgaaga
      901 taccaaaggg tagttcagat tacacggtca cctggaaagg gggccatttt actttttatc
      961 gccgctggcg gtgcaaagtt cacaaagttg tcttacgaag gttgtaaggt aaaacttatc
     1021 gatttgataa tggaaacgca ttagccgaat cggcaaaaat tggttacctt acatctcatc
     1081 gaaaacacgg aggaagtata gatgtccttg attaacacca aaattaaacc ttttaaaaac
     1141 caggcattca aaaacggcga attcatcgaa atcaccgaaa aagataccga aggccgctgg
     1201 agcgtcttct tcttctaccc ggctgacttt actttcgtat gcccgaccga actgggtgac
     1261 gttgctgacc actacgaaga actgcagaaa ctgggcgtag acgtatacgc agtatctacc
     1321 gatactcact tcacccacaa agcatggcac agcagctctg aaaccatcgc taaaatcaaa
     1381 tatgcgatga tcggcgaccc gactggcgcc ctgacccgta acttcgacaa catgcgtgaa
     1441 gatgaaggtc tggctgaccg tgcgaccttc gttgttgacc cgcagggtat catccaggca
     1501 atcgaagtta ccgctgaagg cattggccgt gacgcgtctg atctgctgcg taaaatcaaa
     1561 gcagctcagt acgtagcttc tcacccaggt gaagtttgcc cggctaaatg gaaagaaggt
     1621 gaagcaactc tggctccgtc tctggacctg gttggtaaaa tctaaatttc cttcgtcttt
     1681 cgcgctacca gctccggcgc gaaatacttc ggaaattcac ctaattcttc gggtgctgcg
     1741 gcacccgatt ttcttccccg caccatgatg caagctgcat ccaggtagcc gtagaggccg
     1801 cttgcatgat gatgtttaaa gcccaggaga taaacatgct cgacacaaat atgaaaactc
     1861 aactcaaggc ttaccttgag aaattgacca agcctgttga gttaattgcc acgctggatg
     1921 acagcgctaa atcggcagaa atcaaggaac tgttggctga aatcgcagaa ctgtcagaca
     1981 aagtcacctt taaagaagat aacagcttgc cggtgcgtaa gccgtctttc ctgatcacca
     2041 acccaggttc caaccagggg ccacgttttg caggctcccc gctgggccac gagttcacct
     2101 cgctggtact ggcgttgctg tggaccggtg gtcatccgtc gaaagaagcg cagtctctgc
     2161 tggagcagat tcgccatatt gacggtgatt ttgaattcga aacctattac tcgctctctt
     2221 gccataactg cccggacgtg gtgcaggcgc tgaacctgat gagcgtactg aacccgcgca
     2281 tcaagcacac tgcaattgac ggcggcacct tccagaacga aatcaccgat cgcaacgtga
     2341 tgggcgttcc ggcagtgttc gtaaacggga aagagtttgg tcagggccgc atgacgttga
     2401 ctgaaatcgt tgccaaaatt gatacgggcg cggaaaaacg tgcggcagaa gagctgaaca
     2461 agcgtgatgc ttatgacgta ttaatcgtcg gttccggccc ggcgggtgca gcggcagcaa
     2521 tttactccgc acgtaaaggc atccgtaccg gtctgatggg cgaacgtttt ggtggtcaga
     2581 tcctcgatac cgttgatatc gaaaactaca tttctgtacc gaagactgaa gggcagaagc
     2641 tggcaggcgc actgaaagtt cacgttgatg aatacgacgt tgatgtgatc gacagccaga
     2701 gcgccagcaa actgatccca gcagcagttg aaggtggtct gcatcagatt gaaacagctt
     2761 ctggcgcggt actgaaagca cgcagcatta tcgtggcgac cggtgcaaaa tggcgcaaca
     2821 tgaacgttcc gggcgaagat cagtatcgca ccaaaggcgt gacctactgc ccgcactgcg
     2881 acggcccgct gtttaaaggt aaacgcgtag cggttatcgg cggcggtaac tccggcgtgg
     2941 aagcggcaat tgacctggcg ggtatcgttg agcacgtaac gctgctggaa tttgcgccag
     3001 aaatgaaagc cgaccaggtt ctgcaggaca aactgcgcag cctgaaaaac gtcgacatta
     3061 ttctgaatgc gcaaaccacg gaagtgaaag gcgacggcag caaagtcgtt ggtctggaat
     3121 atcgagatcg tgtcagcggc gatattcaca acatcgaact ggccggtatt ttcgtgcaga
     3181 ttggtctgct gccgaacacc aactggctcg aaggcgcagt cgaacgtaac cgcatgggtg
     3241 agattatcat tgatgcgaaa tgcgaaacca acgtcaaagg cgtattcgca gcgggtgact
     3301 gtacgacggt tccgtacaag cagatcatca tcgccactgg cgaaggtgcc aaagcctctt
     3361 tgagtgcttt tgactacctg attcgcacca aaactgcata agaagaagta agattcacct
     3421 gcaattgctt agccgccggg gtcaaacctg gcggcttttt tatggcatta aaaagccctg
     3481 ccgggatgtt ccaggcaggg cggcataaat acttttatct gttagcgaat gaccagtaca
     3541 ggcagattgg cgtggcggat cacgctcgag gcgttagaac ctaacagatg ggtcgaaatc
     3601 gatgggttgc gagaaccgat aactacaaca tcagccccca gttcttctgc caactcattg
     3661 acttcatccc gcacgctacc aaaacggaca tgttgtttaa tgcgggaagg atcgatggtg
     3721 aagtggctga ccatcgtttg cagacgttct tctgcttcat gttgcagatg ctcttcaaaa
     3781 cgacgcacat cagcggcaaa acggtgcagg ctcaggctgg ctgacccggg tagtacgtga
     3841 agtagatgaa taactccgtc atcctgggcg aggaattcag cgtggcgaac agctttgtcg
     3901 ctcaattcca tttcaaatac atcaactggc ataatgattg tcttatacat aaccctttct
     3961 ccctgttaat catgaacaaa tcattcgcca tgattataat atttatccct gatatttgtc
     4021 tggttctttt tccttacgaa cggtttctgt gattaatata ttctcactga acaccaggaa
     4081 ttctcccaaa acctgcggta ccgcccgttt tcccgctgtg atagctaccc ttaaagactg
     4141 actctttttt gaactgtctc tggaggttgc atgaaagcat tgacttatca cggcccacat
     4201 cacgttcagg tagaaaatgt tcccgatccg ggcattgaac aggctgatga tattattctg
     4261 cgtattacgg caacggcgat ctgtggctct gacctccatc tttatcgagg caaaataccc
     4321 caggttaaac atggcgatat tttgggtcat gaatttatgg gggaagtcgt tgaaaccgga
     4381 aaggacgtaa aaaatttgca aaaaggcgac cgggtggtaa ttccgtttgt cattgcttgt
     4441 ggcgactgtt ttttctgtcg aatgcagcaa tatgccgcct gcgaaaatac caatgcgggt
     4501 aaaggcgctg cgctcaataa aaaacagata ccagctcccg cggcattgtt tggttatagt
     4561 cacctgtatg gtggcgttcc tggtggacag gcggaatatg tccgcgtccc taaagggaat
     4621 gtggggccgt ttaaagtacc gcctttgctt tcagatgata aagccctttt cctttctgat
     4681 attctgccaa cggcatggca ggcagcaaaa aatgcgcaga tccaacaagg ttcaagcgtt
     4741 gcagtctatg gtgccggtcc agtgggattg ttgacaatcg cctgtgcacg gttgctcggt
     4801 gcggaacaga tttttgttgt tgatcatcat ccctaccgct tgcgtttcgc cgctgaccgc
     4861 tacggcgcga tcccgattaa tttcgatgaa gatagcgatc cggcacagtc aattattgaa
     4921 caaacggcag gtcaccgggg cgtggatgca gtaatagacg ccgtcggttt tgaagcgaaa
     4981 ggcagcacca cggaaacggt gctgactaac ctgaaactgg agggcagcag cggtaaagcg
     5041 ttgcgccagt gtattgcggc ggtcaggcgt ggcggcattg ttagcgtacc gggcgtctac
     5101 gctggattta ttcacggttt cctgtttggc gacgcctttg ataaagggtt gacgtttaaa
     5161 atgggacaga cccacgttca cgcatggctg ggtgaattat taccgttaat tgagaaagga
     5221 ttactgaaac cagaagaaat tgttacccac tatatgccgt ttgaagaggc cgcccgggga
     5281 tatgagattt tcgaaaaacg tgaagaggag tgccgtaagg tgattctggt acccggtgca
     5341 caaagcgcag aggcggcgca gaaggcggtt tcaggtctgg tgaatgcgat gccgggggga
     5401 acaatatgat cgtcaggagt ggttttcgag gtaaaggaca gccatgacga taatcgccgc
     5461 cataatcaga aatcctatca ggatgtaaaa tgcttctgcc atggttattc ccacaaacga
     5521 aacggaataa ttttgcagca aaagtgaaca gtgagaacca ggaaaaattg ctgattttgc
     5581 gtaaagagga tgcgagtgca tcctctgggc aaagcgagtt atcgcttgtg cagatgggat
     5641 taaagcaggt agtcgccagc agcttctggc tggtattcga gttccagcac ttcaaggtgg
     5701 gttgcaacgc cgcccggaag ttcccagtga atggaatcgc caacgcgcag ccccagcagt
     5761 gcggcaccta ccggagccat aacggaaagc tgagtattgc tatcggtcat ttttgccgga
     5821 tacaccagcg tgcgcacacg cacttcgcca tcgctaagat tgcggaattt aacccggctg
     5881 ttcattgtca ccacgtcgtg tggcatctct tctggcgaac acatttgggc gcgatccaac
     5941 tctgcgttta acgcgtcggc gattggcaaa ccagcatagg cgggttgctc cagcagaata
     6001 tcgatgcgtt cggcatccag gtcgttaatg atgatagttg gtctggacat ttttactcca
     6061 tgtcgtcggt actgcgagtg tcgcagataa acatacccaa aagaaaaccc tcaccgtcag
     6121 gcggcgaggg tttaactcac atgatgatac tgactgttgc tcactctttg aagtgatttg
     6181 cgtcacattc agggaattcc tcaatgcagc gcattatgta taattcttaa tcgtcacggt
     6241 ttatggaaga cgaattagcg tgttttgtaa atcagatgat taataaccca ctttatcaat
     6301 cacaaagttt ttgccacagt tgcctgggtg tggttgtggc aagaatgagt ttgcagaaag
     6361 cggagcgttg atggcgtcag ctttgatcga gatctgaatt tcagtcaaat acgcagggtt
     6421 accctggcac gttagcttaa ctgctttcac gttctctttt ccccagcttt tggcaaaggc
     6481 ggcgtcaaag tcacggcggc tcactgtttt accgtaatta tccgcaagga attttccggc
     6541 ttcgctctct ttaatttctt gattcaggcg taccatcgta ccgaagtatg cgtccggatc
     6601 aaaaccaaag caggcaccgt gtttggcata ttcgtagcgt tccaggcagg aacgtccgcc
     6661 agctcctggc atgacttcac ttagtttagc ggccgtttcc agtgataacc cggtttccgg
     6721 cgatgaacac atccggctgg cgtgcgcttc tggtagattc gggattgggc gagtagcgca
     6781 accgaagcgc atccagcggc gttcatcaac accacgggca gcaaccgatt taggcaatcc
     6841 tggccacaga ccatgtacgg tcagaaaatc agctttgttg gtcgtttcgg tttgtaggcg
     6901 acattcatct cgttcgttac gatttcgatc gtgttgactc tggcaaaatc cggtttgcca
     6961 ggagagggcc aggacatagc gatcaaaatc gccatactgt tttgcctgca acgctacggc
     7021 gttggcagaa gagaagggaa gcagagaaac cgcgagcaac gcggcgttac gccagaatgc
     7081 tttcataatg ggtgtggaac tcatacatac actgaatact atctattaaa tcataaaaaa
     7141 gcccgccagg tgggcttacc tggcgggcgt gatgatttat tcagcgtttg gcgaacgtat
     7201 tagttccaca tggcgagaat cggccagcca accaacagca acatagagat gtaaatcacc
     7261 ccgaagattg cgccaagacg ccagtaatct tttgatttca catagccaca gccgtaaata
     7321 atcaccccag gaccggttgc atacggcgtc agacagccca tgataccgat agacagcacc
     7381 agcaggatac acagttgttc cattggtacg cccggaatac ctttaccgac ggccagaata
     7441 accgacagca tggttgcggt gtgcgcagac aggctggcaa acaggtagtg tgcaaagtag
     7501 aacaccagaa ccagtacaat caccgttgcg tttggtgaga atccttccag gtgcgtactc
     7561 atggtaccgg cgaaccagtc aataaagcca gaacgagtca ggccgttagc catcacaacc
     7621 agagttgcca ggttgaccag cgtgttccat gcgctgttat agcgggtaat atctttccaa
     7681 ggcacaacgt gcagggccag cattagcgaa actgccagca gaccaactgc agtagcatta
     7741 atgacttcac tgccaaaaac ccacaaacct aagctgagca atacaaggcc aatcagtgtc
     7801 cactctctgc gtgtcagcgc acccatggtt ttcagttcat cacccgccca ggttgccact
     7861 tcttcactgt gtgtgatttc cggtttgtac agcacgtagg aaagccacgg cgcaatgata
     7921 agcaagataa ccccaaccgg caggaagcag aggaaccact gcaaccagct aatctggata
     7981 ccggcaattt tgctgacgaa ctccagaccc agcacgtttg gtgccgcacc ggtgacaaac
     8041 atggacgaac tcagactggt actaatgacc atcatccaca tcaaatagcc gccaatacga
     8101 cgcgcggacg gatcgttggg gaatgattta aacagcggcg gcaggttttt aatgaccgga
     8161 aaaaccgtac ccccggtacg cgcggtgttg gacggtgtaa acggtgccag cagaatgtcg
     8221 ataatgacaa tcgcataacc caacgtcagc gtgcgtttgc ccatgaattt caccaggaaa
     8281 agggcaatgc gacgacctaa cccggaaact tcatacccta atgcaaaaat aaatgcgcca
     8341 aataccagcc ataccgtggt gctggaaaaa ccagccagac cccatttcag cgcctgtttt
     8401 tgcgcattaa acgctgggtc agctaattct ttggcatcaa agagcaggta attactgcca
     8461 ataacgcaaa tagtaaccgc aataaaactg atcgctgttg ccggaattgg ctcgaggatc
     8521 atgccgacaa tcattgccac aaacacagcg aagtaatgcc atgcctgtgg tggcataccg
     8581 tcggggacag ggataagaaa catgacaccc atcaccacca gtggggccaa tagtttccat
     8641 atattatctt ttgctaaaga catacgggtt ctccgaaaat taatatttcc aaatttatca
     8701 agtgcttaaa taattaaatc tgtgctaaaa accaggtaag gatcagcagg tcggcactgc
     8761 cgcctggact gagatttcgt tcgatacact ccctgtcgaa ctgccggaga taatcgagat
     8821 cggcgggggt tcgaatgccc cctttttgca ataatgtttg cgcctcgcgc tgtagccagc
     8881 gcaggccccc ctcgccaccg cgcgatgcaa cgttggtatc gccgttgatc gccatcagta
     8941 ggagcaaggt atcgagcaat gccagttcag gatctaaccc ctgatccagc agagtgaggt
     9001 aatgcggcaa ggcgtgattg atcaccagtg gataacccgc ttcggcttca ccgcgtgcgc
     9061 cggtaaggcc aagctgctgg tacaaccgtt gacctgccgt cagttgtgaa ttattggtac
     9121 gcagttctcg atcggtcagg ccacggcaga aacttgccgc cgtagaacaa acggttgttg
     9181 gcgttaccgg ttggttgagt tgaagcaaac ggccaattgc cgcacatagt agccctaaag
     9241 aaaaaatgct gcctttatgc gtgtttacgc ccgcagtggc gcggaacata tcaccttcgc
     9301 aagccatacc aattgggcgt aatccgtgga gtaccgcttc tggtgctatt tccgcactac
     9361 aggcaccaaa ttcaatgaaa cggggtagcc agccctgaat cgccagcgcg ctgcggtgga
     9421 aatcttccag cgccatatct ttatgtgcac cgcagttaat ccgatccacg aggcctggtt
     9481 tcggtgacag attgacttca gtcagcatgg cgcgccagcc cagcagggcg tactcatcga
     9541 tcaatgacgt cgcaagcttt gtggttttag ttgacgttgc aggcatcgac atcgttcagc
     9601 agtgcctcca tgcggttgag taaatcggtc agttgatggg tttttccacg cgcgcagacg
     9661 gctgcgcttt gctcgcacaa caggcagcgg cgaggcggca gtgaatagtc gcggcgggag
     9721 agaatttcgc cttcgggcgt caggacatcg atatcccata accgcccgag aggatgacta
     9781 tgttcaagct caatggtggc gagcttgagg tcgcgagccg gggcggcaat gctcaacatg
     9841 ccctccggcc cgctggcgga aaccagtgca gcctgctcct gaatttgcca gccctgtttt
     9901 gcggctaagg cacgcaaggc tgtcacgcca tgattaaaaa ttcggcgtgt gacctcgctg
     9961 tctttaatcg gcccaggcgc aaccacggta aaggagacca gtggaacagg atggcgcttg
    10021 agccagacgt gttgccgtgc ttgcctttca tcccggctga cgagcagctc gggaattgac
    10081 accgcatggt ggctggcgag ttcaggaagc aggtgcatgg cttattcctt cacctgatgc
    10141 acaacatcga tcaccgagcc atcgcggtaa cgcacaacgg caacgacgcg gtcggtgaat
    10201 tcaatcggct gtggttcacc ggtcagcaga cgcgcgcgtt cgcgcagcca ctcaatggaa
    10261 accactttaa tgcccgcttc ctgcagacgt tctgccagtt ccggacgtgc cgggttaact
    10321 gcgataccgt ggtctgtgac cagaatatcg acactggagc ctggggtgat gcaggtcagc
    10381 acgttatcca ccagagtcgg aatacgaccg cgtaccagcg gcgcgacgat gatggaaagc
    10441 gcagaggcaa tcgcggtatc gcagtgacca ccggaagcac cacgcagtac gccgtcagag
    10501 ccggtcagca cgttaacgtt gaactgggtg tcaatttcca gcgcgctcag taccaccacg
    10561 tcgagacgat caaccgatgc gcctttcgaa ccccagttag cgtactggtt ggcgctgatt
    10621 tcgatgtgat tggggttacg ggccagcgat tgcgcagcat ggctgtcaaa gctctgcaca
    10681 tccagcagtt tgcggatcag acctttttcg tgcagatcaa ccatcgtcgc ggtaataccg
    10741 ccgagggcga agtcggcgcg aatatcgcgg ctacgcattt tgtcttccag gaaacgggtt
    10801 accgccagcg atgcgccgcc ggtgccggtt tgcatggaga aaccttcttt gaagtagcca
    10861 gagttgacaa tcacatccgc agcgctacgg gcaataagca gttcgcgcgg gttagtggtc
    10921 atacgggtcg cgccagcgcc gatttttgca gcatcgccaa cgcggtcaac tttgacgatc
    10981 aaatcaacct gatcttgctc aatgcttgcc ggattatgcg gataaggcag cagttcttcg
    11041 gtaagcatca caacctgttt tgcgttgtcg gcatcaacca ttgcatagcc gagggagccg
    11101 cagcaggctt taccggtgta accgttggca ttaccgaatt catcacagga cgggacgccg
    11161 aggaaagcca cgtcgatatt cagttcgccg ctctgtacca gatgcacacg tccgccgtga
    11221 gagtggattt gcaccggttc cgcaagcaga ccacgggaaa tttcttctgc cagtggaccg
    11281 cgcaggccgg aggtataaat gcgggtaacc acgccctggc gaatgtgttc taccagtggc
    11341 gcatggcaat cactcaggga gctggacgcc agggtcaggt ttttaaagcc catcttagcg
    11401 atgacgtcca tcaccatatt gacggtcagg tcaccgccac ggaaagcgtg atggaaggaa
    11461 accgtcatgc cgtcctgtaa accagagcga cgaatcgctt cttcctggtt ggcgcacagt
    11521 ttgcgatcgc gcgctttttc agcctggtag gtttgctttg gtgagttctg gaaagcggca
    11581 agatcgcatt cagcgcgacg attccaggcc gctacccgtt cttgtcgttg agattgttca
    11641 attttctgcg tcattttgat tgccttattc ttcgcggatg ccggaaagtt ctgcacggga
    11701 gagcaccaga cgggcacgat cgataaccgg accgtccacc atcttgccgt tcagggaaac
    11761 cacgccgagg ccttcgcgag cggcggcttc agcggcttct acgacgcggc gggcgtgatc
    11821 cacttctttc tgggtcggtg cgtagaggtt gtgcaacaga tcaatctgac gcgggttgat
    11881 cagcgatttg ccgtcaaagc ccagctgttt gatgtgggcg gcttcttgca ggaatccggc
    11941 ttcgttgtta gcgtcggaat agacggtatc gaacgcctga atacccgcag agcgcgcggc
    12001 ctgcaaaatg gaacagcgtg cgaacagcag ttcagttcct tccggggagc gttctgtacg
    12061 caggttgcgc acatagtctt ctgcaccgag ggcgataccg atcaaacgct cggaagcgtg
    12121 agcgatttcc actgcgcggg taatccccag cggagattca atcgccgcca gcaggccggt
    12181 gctgccgggt tcacgaccac aggctttttc gatacgcagg atctcttttt cgatatccag
    12241 aacatcctga gcggtatcgg ttttcggcag acgcacaacg tccgcaccac cgcgaacgac
    12301 ggcttccagg tcgttgacac cccattcgga atccagcgcg ttgacacgca caatggtttc
    12361 aatatcgcga tacagcggat gttgcagcgc gtggtaaacc atgcggcggg cggtgtcttt
    12421 ttcacgcaat gctacggagt cttcgaggtc aaacatcagg gcatcagccg ggtagatgaa
    12481 ggagttgctg accatcgcgg cattggcacc aggcacaaac aacatgctgc ggcgggtgcg
    12541 agttttacgt tgttgcagcg aagcggaaat cattggcaat cctcccatgg cagagccggg
    12601 ataccgctgg cgcgtgccag cagggcttcc agtcgtgcac gtaaaatgca gtccagtgcg
    12661 cctttgtcat cgacattcag ctgtacgccg cgcacgttgt agcgggcgag aacgtccaga
    12721 atggtggtgc gaattgcatc gccaaactgt ttctcaacgc tgctattgat ttgcaggtcg
    12781 atatcctgcg tatcgagtgg ggcgatgcgt atcatcacat ccccagactc aagggtgcct
    12841 gcaacggcgg gctggtttat tttcattttt cacctgtttc tcatgcgggg gtcttttgac
    12901 gagctgccgc gtcctggcgg gagtgctcaa gcaggttctg caaataatgc agcgtgactg
    12961 cagggaccag cggcgcgata gccgtgagat cgtttttcgc cagcagttga cgtacccggg
    13021 aagcggatat cggcatctcc tggtaacgca gccgctcaat ttcaaccagt tcgatgggcg
    13081 gtgcggagat agtcggcgtt tccagccagt agcgcatatc ctggttgtac tgggcggtaa
    13141 cgcgacaaaa gggttcagta ccgacaaagc ggtgagttac acccagcgcg ggagcgaggt
    13201 actgacggaa aatcttcaga tcaatttcgg tgtaacaatg gttaatgacg ctctgttctt
    13261 taatgaagta gcaagggaac gtagcgcggg agatgatgta ttcggagccg cgatgcacag
    13321 tcaggcgtgg aatatcggcg gtgcctttta acaccagatc caaccggtct tcatagggga
    13381 agcgtgaaga atcttctttg actaaaaaca gatgcaacca gtcgcactgt gccgcagctt
    13441 gttgaatcag ataacggtga ccattcgtga agggattggc gttcatcaca atgcagccaa
    13501 tcttgttccc tggatgacga aattttttca gcgattcggc atagcgtttc agccgcgtgg
    13561 cgctgttttc catcagcacc atcacgccgg gtacgctggt cagcgtggaa aaaccgcact
    13621 ggcggaacag cgcctcgtat tcggttttgg tataaataaa cagatgcgtg ctgtgccgct
    13681 cataggcgag gtttatcaat tcagtggcta atgtcagcgc cagtccttca ccgcgaacgg
    13741 attcactgat agcaacgcat ttaataatat ttccggca
//
LOCUS       DADMKF010000109        13716 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.111, whole genome shotgun sequence.
ACCESSION   DADMKF010000109 DADMKF010000000
VERSION     DADMKF010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13716
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.111"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(345..608)
                     /gene="rpsT"
                     /locus_tag="JNC24_002905"
     CDS             complement(345..608)
                     /gene="rpsT"
                     /locus_tag="JNC24_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HAY9159602.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
     gene            711..929
                     /locus_tag="JNC24_002906"
     CDS             711..929
                     /locus_tag="JNC24_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 domain-containing protein"
                     /protein_id="HAY9159603.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGHFLPVICAMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            937..1878
                     /gene="ribF"
                     /locus_tag="JNC24_002907"
     CDS             937..1878
                     /gene="ribF"
                     /locus_tag="JNC24_002907"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HAY9159604.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKASMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            1921..4737
                     /gene="ileS"
                     /locus_tag="JNC24_002908"
     CDS             1921..4737
                     /gene="ileS"
                     /locus_tag="JNC24_002908"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HAY9159605.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFAVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELSAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            4737..5231
                     /gene="lspA"
                     /locus_tag="JNC24_002909"
     CDS             4737..5231
                     /gene="lspA"
                     /locus_tag="JNC24_002909"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein signal peptidase"
                     /protein_id="HAY9159606.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVILAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSR
                     AKKQ"
     gene            5356..5805
                     /gene="fkpB"
                     /locus_tag="JNC24_002910"
     CDS             5356..5805
                     /gene="fkpB"
                     /locus_tag="JNC24_002910"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HAY9159607.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            5807..6757
                     /gene="ispH"
                     /locus_tag="JNC24_002911"
                     /gene_synonym="lytB"
     CDS             5807..6757
                     /gene="ispH"
                     /locus_tag="JNC24_002911"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HAY9159608.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKRAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            6823..7737
                     /gene="rihC"
                     /locus_tag="JNC24_002912"
     CDS             6823..7737
                     /gene="rihC"
                     /locus_tag="JNC24_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HAY9159609.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPIGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLSQCPECKPYIRRLVIMGGSSGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVKVALDLDVKGFQQWVAEVLALAS"
     gene            7904..8725
                     /gene="dapB"
                     /locus_tag="JNC24_002913"
     CDS             7904..8725
                     /gene="dapB"
                     /locus_tag="JNC24_002913"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HAY9159610.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAVKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKENGLFDMRDVLNLNNL"
     gene            9181..10329
                     /locus_tag="JNC24_002914"
     CDS             9181..10329
                     /locus_tag="JNC24_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase small subunit"
                     /protein_id="HAY9159611.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRTDKPAFSFQGHPEASPGPHDAAPLFDHFIELIEQYRKTAK"
     gene            10347..13568
                     /gene="carB"
                     /locus_tag="JNC24_002915"
     CDS             10347..13568
                     /gene="carB"
                     /locus_tag="JNC24_002915"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HAY9159612.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     ERLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
BASE COUNT         3210 a         3426 c         3846 g         3234 t
ORIGIN      
        1 agatctaata aggagatatt tatgaatggt taaagtgtat aggaatttaa ttcaaaaata
       61 gagtggtctt aagtttaata cctcaatgtg tatcacaata tccacattct ttgtagggga
      121 gtctggagat tgagtagata ttcttgttca gaatgtatca gccgatggtt ctacgattct
      181 taagccacga agagttcaga aagtgtaacg gcatgtctgc aaaagccagc atcttttgac
      241 tatctggcaa ccggtagtgt gttctctcac gcatcataaa agcagcaggc ataaaaaaaa
      301 cccgcttgcg cgggcttttt cacaaagctt cagcaaattg gcgattaagc cagtttgttg
      361 atctgtgcag tcaggttagc cttatgacgt gcagctttgt ttttgtggat cagaccttta
      421 gcagcctgac ggtccacgat cggttgcatt tcgttaaatg ctttctgtgc agcagctttg
      481 tcgccagctt cgatagctgc gtatactttc ttgatgaaag tacgcatcat agagcgacgg
      541 cttgcgttgt gcttacgagc cttttcagac tgaatggcgc gcttcttagc tgatttgata
      601 ttagccaagg tccaactccc aaatgtgttc tatatggaca attcaaaggc cgaggaatat
      661 gcccttttag ccttcttttg tcaatggatt tgtgcaaata agcgccgtta atgtgccggc
      721 actcgttacg tagtgatggc gcaggattct accagcttgc ggggtgtgaa tacagctttt
      781 ccgcgataaa aattgcagca ggcggtcact ttcttcccgt gatttgcgcc atggcaatga
      841 aaagccactt ctttctgatt tcggtactca atcgccggtt aaccttgacc gctgtacaag
      901 gtatactcgg acgattttca ctgttttgag ccagacatga agctgatacg cggcatacat
      961 aatctcagcc aggccccgca agaagggtgt gtgctgacta ttggtaattt cgacggcgtg
     1021 catcgcggtc atcgcgcgct gttacagggc ttgcaggaag aagggcgcaa gcgcaactta
     1081 ccggtgatgg tgatgctttt tgaacctcaa ccactggaac tgtttgctac cgataaagcc
     1141 ccggcaagac tgacccggct gcgggaaaaa ctgcgttacc ttgcagagtg tggcgttgat
     1201 tacgtgctgt gcgtgcgttt cgacaggcgt ttcgcggcgt taaccgcgca aaatttcatc
     1261 agcgatcttc tggtgaagca tttgcgcgta aaatttcttg ccgtagggga tgatttccgc
     1321 tttggcgctg gtcgtgaagg cgatttcttg ttattacaga aagctagcat ggaatacggc
     1381 ttcgatatca ctagtacgca aactttttgc gaaggtggcg tgcgtattag cagcaccgct
     1441 gtgcgtcagg cgctggcgga tgacaatctg gctctggcag aaagtttact ggggcacccg
     1501 tttgccatct ccgggcgtgt agtccacggt gatgaattag ggcgcactat aggtttcccg
     1561 acggcgaatg taccgctacg ccgtcaggtt tccccggtga aaggggttta tgcggtagaa
     1621 gtgctgggcc tcggtgaaaa gccgttaccc ggcgtggcaa acatcggaac acgcccaacg
     1681 gttgccggta ttcgccagca gctggaagtg catttgttag atgttgcaat ggacctttac
     1741 ggtcgccata tacaagtagt gctgcgtaaa aaaatacgca atgagcagcg atttgcgtcg
     1801 ctggacgaac tgaaagcgca gattgcgcgt gatgaattaa ccgcccgcga attttttggg
     1861 ctaacaaaac cggcttaagc ctgttatgta atcaaaccga aacacggaac cgagaatctg
     1921 atgagtgact ataaatcaac cctgaatttg ccggaaacag ggttcccgat gcgtggcgat
     1981 ctcgccaagc gcgaaccggg aatgctggcg cgttggactg atgatgatct gtacggcatc
     2041 atccgtgcgg ctaaaaaagg caaaaaaacc ttcattctgc atgatggccc tccttatgcg
     2101 aatggcagca ttcatattgg tcactcggtt aacaagattc tgaaagacat tatcgtgaag
     2161 tccaaagggc tttccggtta tgactcgccg tatgtgcctg gctgggactg ccacggtctg
     2221 ccgatcgagc tgaaagtaga gcaagaatac ggtaagccgg gtgagaaatt caccgccgcc
     2281 gagttccgcg ccaagtgccg cgaatacgcg gcgacccagg ttgacggtca acgcaaagac
     2341 tttatccgtc tgggcgtgct gggcgactgg tcgcacccgt acctgaccat ggacttcaaa
     2401 actgaagcca acatcatccg cgcgctgggc aaaatcatcg gcaacggtca cctgcacaaa
     2461 ggcgcgaagc cagttcactg gtgcgttgac tgccgttctg cgctggcgga agcggaagtt
     2521 gagtattacg acaaaacttc tccgtccatc gacgttgctt ttcaggcggt cgatcaggat
     2581 gcactgaaag caaaatttgc cgtaagcaac gttaacggcc caatctcgct ggtgatctgg
     2641 accactacgc cgtggactct gcctgcgaac cgcgcaatct ctattgcacc tgatttcgac
     2701 tatgcgctgg tgcagatcga cggtcaggcc gtgattctgg cgaaagatct ggttgaaagc
     2761 gtaatgcagc gtatcggcgt gaccgattac accattctcg gcacggtaaa aggtgcggag
     2821 cttgagttgc tgcgctttac ccatccgttt atgggcttcg acgttccggc aatcctcggc
     2881 gatcacgtta ccctagatgc cggtaccggt gccgttcaca ccgcgcctgg ccacggcccg
     2941 gacgactatg tgatcggtca gaaatacggc ctggaaaccg ctaacccggt tggcccggac
     3001 ggcacttatc tgccgggcac ttatccgacg ctggatggcg tgaacgtctt caaagcgaac
     3061 gacatcgtcg ttgcgctgct gcaggaaaaa ggcgcgctgc tgcacgttga gaaaatgcag
     3121 cacagctatc cgtgctgctg gcgtcacaaa acgccgatca tcttccgcgc gacgccgcag
     3181 tggttcgtca gcatggatca gaaaggtctg cgtgcgcagt cactgaaaga gatcaaaggc
     3241 gtgcagtgga tcccggactg gggccaggcg cgtatcgagt cgatggttgc taaccgtcct
     3301 gactggtgta tctcccgtca gcgcacctgg ggcgtaccga tgtcactgtt cgtgcacaaa
     3361 gacacggaag agctgcatcc gcgtactctc gaactaatgg aagaagtggc aaaacgcgtt
     3421 gaagttgacg gcatccaggc gtggtgggat cttgatgcga aagagatcct cggcgatgaa
     3481 gctgatcagt acgtgaaagt gccggacaca ttggatgtat ggtttgactc cggatctacc
     3541 cactcttctg ttgttgacgt gcgtccggaa tttgccggtc acgcagcgga catgtatctg
     3601 gaaggttctg accagcaccg tggttggttc atgtcttccc taatgatctc caccgcgatg
     3661 aaaggcaaag cgccgtatcg tcaggtactg acccacggct ttaccgtgga tggtcagggt
     3721 cgcaagatgt ctaaatccat cggcaatacc gtttcgccgc aggatgtgat gaacaaactg
     3781 ggcgcggata ttctgcgtct gtgggtggca tcaaccgact acaccggtga aatggccgtt
     3841 tctgacgaga tcctgaaacg tgctgccgat agctatcgtc gtatccgtaa caccgcgcgc
     3901 ttcctgctgg caaacctgaa cggttttgat ccagcaaaag atatggtgaa accggaagag
     3961 atggtggtac tggatcgctg ggccgtaggt tgtgcgaaag cggcacagga agacatcctc
     4021 aaggcgtacg aagcatacga tttccacgaa gtggtacagc gtctgatgcg cttctgctcc
     4081 gttgagatgg gttccttcta cctcgacatc atcaaagacc gtcagtacac cgccaaagcg
     4141 gacagtgtgg cgcgtcgtag ctgccagact gcgctgtatc acatcgcaga agcgctggtg
     4201 cgctggatgg caccaatcct ctccttcacc gctgatgaag tgtggggcta cctgccgggc
     4261 gaacgtgaaa aatacgtctt caccggtgag tggtacgaag gcctgtttgg cctggcagac
     4321 agtgaagcga tgaacgatgc gttctgggac gagctgttga aagtgcgtgg cgaagtgaac
     4381 aaagtcattg agcaagcgcg tgccgacaag aaagtgggtg gctcgctgga agcggcagta
     4441 accttgtatg cagaaccgga actgtcggcg aaactgaccg cgctgggcga tgaattacga
     4501 tttgtcctgt tgacctccgg cgctaccgtt gcagactata acgacgcacc tgctgatgct
     4561 cagcagagcg aagtactcaa agggctgaaa gtcgcgttga gtaaagccga aggtgagaag
     4621 tgcccacgct gctggcacta cacccaggat gtcggcaagg tggcggaaca cgcagaaatc
     4681 tgcggccgct gtgtcagcaa cgtcgccggt gacggtgaaa aacgtaagtt tgcctgatga
     4741 gtcaatcgat ctgttcaaca gggctacgct ggctgtggct ggtggtagtc gtgctgatta
     4801 tcgatctggg cagcaaatac ctgatcctcc agaactttgc tctgggggat acggtcccgc
     4861 tgttcccgtc gcttaatctg cattatgcgc gtaactatgg cgcggcgttt agtttccttg
     4921 ccgatagcgg cggctggcag cgttggttct ttgccggtat tgcgattggt attagcgtga
     4981 tcctggcagt gatgatgtat cgctcgaagg ccacgcagaa gctaaacaat atcgcttacg
     5041 cgctgattat tggcggcgcg ctgggcaacc tgttcgaccg cctgtggcac ggcttcgttg
     5101 tcgatatgat cgacttctac gtcggcgact ggcacttcgc caccttcaac cttgccgata
     5161 ctgccatctg tgtcggtgcg gcactgattg tgctggaagg ttttttgcct tctagagcga
     5221 aaaaacaata ataaaccctg ccggatgcga tgctgacgca tcttatccgg cctacagatt
     5281 gctgcgaaat cgtaggccgg ataaggcgtt tacgccgcat ccggcaaaaa tcctcaaata
     5341 taagagcaaa cctgcatgtc tgaatctgta cagagcaata gcgccgtcct ggtgcacttc
     5401 acgctaaaac tcgacgatgg caccaccgcc gagtctaccc gcaacaacgg taaaccggcg
     5461 ctgttccgcc tgggtgatgc ttctctttct gaagggctgg agcaacacct gttggggctg
     5521 aaagtgggcg ataaaaccac cttctcgttg gagccagatg cggcgtttgg cgtgccgtca
     5581 ccggacctga ttcagtactt ttcccgccgt gaatttatgg atgcaggcga gccagaaatt
     5641 ggcgcaatca tgctttttac cgcaatggat ggcagtgaga tgcctggcgt gatccgcgaa
     5701 attaacggcg actccattac cgttgatttc aaccatccgc tggccgggca gaccgttcat
     5761 tttgatattg aagtgctgga aatcgatccg gcattggagg cgtaacatgc agatcctgtt
     5821 ggccaacccg cgtggttttt gtgccggggt agaccgcgct atcagcattg ttgaaaacgc
     5881 gctggccatt tacggcgcac cgatatatgt ccgtcacgaa gtggtacata accgctatgt
     5941 ggtcgatagc ttgcgtgagc gtggggctat ctttattgag cagattagcg aagtaccgga
     6001 cggcgcgatc ctgattttct ccgcacacgg tgtttctcag gcggtacgta acgaagcaaa
     6061 aagccgcgat ttgacggtgt ttgatgccac ctgtccgctg gtgaccaaag tgcatatgga
     6121 agtcgcccgc gccagccgtc gtggcgaaga gtcgattctc atcggccacg ccgggcatcc
     6181 ggaagtggaa ggtacaatgg gccagtacag taacccggaa gggggaatgt atctggtcga
     6241 atcgccggac gatgtgtgga aactgacggt caaaaacgaa gagaagctct cctttatgac
     6301 ccagaccacg ctgtcggtgg atgacacgtc tgatgtgatc gacgcgctgc gtaaacgctt
     6361 cccgaaaatt gtcggtccgc gcaaagatga catctgctac gccacgacta accgtcagga
     6421 agcggtacgc gccctggcag aacaggcgga agttgtgttg gtggtcggtt cgaaaaactc
     6481 ctccaactcc aaccgtctgg cggagctggc ccagcgtatg ggcaaacgcg cgtttttgat
     6541 tgacgatgcg aaagatatcc aggaagagtg ggtgaaagag gttaaatgcg tcggcgtgac
     6601 tgcgggcgca tcggctccgg atattctggt gcaaaatgtg gtggcacgtt tgcagcagct
     6661 gggcggtggt gaagccattc cgctggaagg ccgtgaagaa aacattgttt tcgaagtgcc
     6721 gaaagagctg cgtgtcgata ttcgtgaagt cgattaagtc attagcagcc taagttatgc
     6781 gaaaatgccg gtcttgttac cggcattttt tatggagaaa acatgcgttt acctatcttc
     6841 ctcgatactg accccggcat tgacgatgcc gtcgccattg ccgccgcgat ttttgccccc
     6901 gaactcgacc tgcaactgat gaccaccgtc gcgggtaatg tctcggttga gaaaaccacc
     6961 cgcaacgccc tgcaactgct gcatttctgg aatgcggaga ttccgctcgc ccagggagcc
     7021 gctgtgccgc tggtacgcgc accgcgtgat gcagcatctg tgcacggcga atcgggaatg
     7081 gctggctacg actttgttga gcacaaccga aagccaatcg ggataccagc gtttctggcg
     7141 attcgtgatg ccctgatgcg tgcaccagag cctgttaccc tggtggccat tggcccgtta
     7201 accaatattg cgctgttact ttcacaatgc ccggaatgta aaccgtatat tcgtcgtctg
     7261 gtgatcatgg gcggttcttc tggtcgcggc aactgtacac caaatgccga gtttaatatc
     7321 gctgccgatc cagaagcggc tgcctgtgtc ttccgtagcg gaatcgaaat cgtcatgtgc
     7381 ggtctggatg tcaccaatca ggcaatatta acccctgact atctcgctac tttgccggag
     7441 ttgaatcgca ccggaaaaat gcttcatgca ctgtttagcc actaccgtag cggcagtatg
     7501 caaagcggtc tgcgaatgca cgatctctgc gccatcgcct ggctggtgcg cccggactta
     7561 ttcaccctca agccgtgttt tgtcgcggta gaaactcaag gcgaatttac ctcaggcacg
     7621 acggtggttg atatcgacgg ttgcctgggc aaaccagcca atgtaaaggt ggcattggat
     7681 ctggatgtga aaggcttcca gcagtgggtg gctgaggtgc tggctctggc gtcgtaacct
     7741 gtcacatgtt atcggcatgc agtcattcat cgactcatgc ctttcactga tatccctccc
     7801 tgtttatcat taatttctaa ttatcagcgt ttttggctgg cggcgtagcg atgcgctggt
     7861 tactctgaaa acggtctatg caaattaaca aaagagaata gctatgcatg atgcaaacat
     7921 ccgcgttgcc atcgcgggag ccggggggcg tatgggccgc cagttgattc aggcggcgct
     7981 ggcattagaa ggcgtgcagc tgggcgcggc gctggagcgc gaaggatctt ctttactggg
     8041 cagcgacgcc ggtgagctgg ctggtgcagg gaaaacaggc gttaccgtgc aaagcagcct
     8101 cgatgcggta aaagatgatt ttgatgtgtt tatcgatttt acccgtccgg aaggtacgct
     8161 gaaccatctc gctttttgtc gccagcatgg caaagggatg gtgatcggca ctacggggtt
     8221 tgacgaagcc ggtaaacagg cgattcgtga cgccgccgcc gatattgcga ttgtctttgc
     8281 cgccaatttt agcgttggcg ttaacgtcat gcttaagctg ctggagaaag cagccaaagt
     8341 gatgggtgac tacaccgata tcgaaattat tgaagcacat catagacata aagttgatgc
     8401 gccgtcaggc accgcactgg caatgggaga ggcgatcgcc cacgcccttg ataaagatct
     8461 gaaagattgc gcggtctaca gtcgtgaagg ccacaccggt gaacgtgtgc ctggcaccat
     8521 tggttttgcc accgtgcgtg caggtgacat cgttggtgaa cataccgcga tgtttgccga
     8581 tattggcgag cgtctggaga tcacccataa ggcgtccagc cgtatgacat ttgctaacgg
     8641 cgcggtaaga tcggctttgt ggttgagtgg taaggaaaat ggtctttttg atatgcgaga
     8701 tgtacttaat ctcaataatt tgtaaccaca aaatatttgt tatggtgcaa aaataacaca
     8761 tttaatttat tgattataaa gggctttaat ttttggccct tttattttta gtgttatgtt
     8821 tttaaatatt ctataagtgc caaaaattac atgttttgtc ttctattttt gttgttttaa
     8881 tgtaaatttt gaccatttgg tccacttttt cctgctcgtt tttatttcat gcaatcttct
     8941 tgctgcgcaa gcgttttcca gaacaggtta tatgatcttt ttgtcgctta atgcctgtaa
     9001 aatatgcatg agccacaaaa taatataaaa aataccgcca ttaagttgac ttttagcgcc
     9061 catatctcca gaatgccgcc gtttgccaga aattcgtcgg taagcagatt tgcattgatt
     9121 tacgtcatca ttgtgaatta atatgcaaat aaagtgagtg aatattctct ggagggtgtt
     9181 ttgattaagt cagcgctatt ggttctggaa gacggaaccc agtttcacgg tcgggccata
     9241 ggggcaacag gttcggcggt tggggaagtc gttttcaata cttcaatgac cggttatcaa
     9301 gaaatcctca ctgatccttc ctattctcgt caaatcgtta ctcttactta tccccatatt
     9361 ggcaatgtcg gcactaatga cgccgatgaa gaatcttctc aggtacatgc acaaggtctg
     9421 gtgattcgcg atctgccgct gattgccagc aacttccgta ataccgaaga cctctcttct
     9481 tacctgaagc gccataacat cgtggcgatt gccgatatcg atacccgtaa gctgacgcgt
     9541 ttactgcgcg agaaaggcgc acagaatggc tgcattatcg caggcgataa cccggatgcg
     9601 gcgttggcgt tagaaaaagc ccgcgcgttc ccaggtctga atggcatgga tctggcaaaa
     9661 gaagtgacca ccgcagaagc ctatagctgg acacaaggga gctggacgtt gaccggcggc
     9721 ctgccagaag cgaaaaaaga agacgagctg ccgttccacg tcgtggctta tgattttggt
     9781 gccaagcgca acatcctgcg gatgctggtg gatagaggct gtcgcctgac catcgttccg
     9841 gcgcaaactt ctgcggaaga tgtgctgaaa atgaatccag acggcatctt cctctccaac
     9901 ggtcctggcg acccggcccc gtgcgattac gccattaccg ccatccagaa attcctcgaa
     9961 accgatattc cggtattcgg catctgcctc ggtcatcagc tgctggcgct ggcgagcggt
    10021 gcgaagactg tcaaaatgaa atttggtcac cacggcggca accatccggt taaagatgtg
    10081 gagaaaaacg tggtgatgat caccgcccag aaccacggtt ttgcggtgga tgaagcaaca
    10141 ttacctgcaa acctgcgtgt cacgcataaa tccctgttcg acggtacgtt acagggcatt
    10201 catcgcactg ataaaccggc gttcagcttc cagggtcacc cggaagccag ccctgggcca
    10261 cacgacgcag cgccgttgtt cgaccacttt atcgagttaa ttgagcagta ccgtaaaacc
    10321 gctaagtaat caggagtaaa agagccatgc caaaacgtac agatataaaa agtatcctga
    10381 ttctgggtgc gggcccgatt gttatcggtc aggcgtgtga gtttgactac tctggcgcgc
    10441 aagcatgtaa agccctgcgc gaagagggtt accgcgtcat tctggtgaac tccaacccgg
    10501 cgaccatcat gaccgacccg gaaatggccg atgcgaccta catcgagccg attcactggg
    10561 aagtagtacg caagattatt gaaaaagagc gcccggacgc ggtgctgcca acgatgggcg
    10621 gtcagacggc gctgaactgc gcgctggagc tggagcgtca gggcgtgttg gaagagttcg
    10681 gcgtgactat gattggtgcg accgccgatg cgattgataa agcagaagac cgccgtcgtt
    10741 tcgacgtagc gatgaagaaa attggtctgg aaaccgcgcg ttccggtatc gcacacacga
    10801 tggaagaagc gctggcggtt gccgctgacg tgggcttccc gtgcattatt cgcccatcct
    10861 ttaccatggg cggtagcggc ggcggtatcg cttataaccg cgaagagttt gaagaaattt
    10921 gcgcccgcgg tctggatctc tccccaacca aagagctgct gattgatgag tcgctgatcg
    10981 gctggaaaga gtacgagatg gaagtggtgc gtgataaaaa cgacaactgc atcatcgtct
    11041 gctctatcga aaactttgat gcgatgggca tccataccgg tgactccatc actgtcgcgc
    11101 cagcccaaac gctgaccgac aaagaatatc aaatcatgcg taacgcctcg atggcggtgc
    11161 tgcgtgaaat cggcgttgaa accggtggtt ccaatgtcca gtttgcggtg aacccgaaaa
    11221 acggtcgcct gattgttatc gaaatgaacc cacgcgtgtc ccgttcttcg gcgctggcgt
    11281 cgaaagcgac cggtttcccg attgctaaag tggcggcgaa actggcggtg ggttacaccc
    11341 tcgacgaact gatgaacgac atcactggcg gacgtactcc ggcctccttc gagccgtcca
    11401 tcgactatgt ggttactaaa attcctcgct tcaacttcga aaaattcgct ggtgctaacg
    11461 accgtctgac cactcagatg aaatcggttg gcgaagtgat ggcgattggt cgcacgcagc
    11521 aggaatccct gcaaaaagcg ctgcgcggcc tggaagtcgg tgcgactgga ttcgacccga
    11581 aagtgagcct cgatgacccg gaagcgttaa ccaaaatccg tcgcgaactg aaagatgctg
    11641 gcgcagagcg tatctggtac atcgccgatg ccttccgtgc gggcctgtct gtggacggcg
    11701 tgttcaacct gaccaatatt gaccgctggt tcctggtaca gattgaagaa ctggtgcgtc
    11761 tggaagagaa agtagcggaa gtgggcatca ctggcctgaa cgctgaattc ctgcgccagc
    11821 tgaaacgcaa aggctttgcc gatgcgcgtc tggcaaaact ggcgggcgtg cgcgaagcgg
    11881 aaatccgcaa gctgcgcgac cagtatgacc tgcacccggt ttataagcgc gtggatacct
    11941 gtgcggcaga gttcgccacc gacaccgctt acatgtactc cacttatgaa gaagagtgcg
    12001 aagcgaatcc gtctaccgac cgtgaaaaaa tcatggttct cggcggcggc ccgaaccgta
    12061 tcggtcaggg tatcgaattc gactactgct gtgtacacgc ctcgctggcg ctgcgcgaag
    12121 acggttacga aaccattatg gttaactgta acccggaaac cgtctccacc gactacgaca
    12181 cttccgatcg cctctacttc gagccggtaa ctctggaaga tgtgctggaa atcgtgcgta
    12241 ttgagaagcc gaaaggcgtt atcgttcagt acggcggtca gaccccgctg aaactggcgc
    12301 gcgcgctgga agctgctggc gtaccggtta tcggcaccag cccggatgct atcgaccgtg
    12361 cagaagaccg tgaacgcttc cagcatgcgg ttgagcgtct gaaactgaaa caaccggcga
    12421 acgccaccgt taccgctatt gaaatggcgg tagagaaggc gaaagagatt ggctacccac
    12481 tggtggtacg tccgtcttac gttctcggcg gtcgggcgat ggaaatcgtc tatgacgaag
    12541 ctgacctgcg tcgctacttc cagacggcgg tcagcgtgtc taacgatgcg ccagtgttgc
    12601 tggatcactt ccttgatgac gcggtagaag ttgacgtgga tgccatctgc gacggcgaaa
    12661 tggtgctgat tggcggcatc atggagcaca tcgagcaggc gggcgtgcac tccggtgact
    12721 ccgcatgttc tctgccagcc tacaccttaa gtcaggaaat tcaggatgtg atgcgccagc
    12781 aggtgcagaa actggccttc gaattgcagg tgcgcggtct gatgaacgtg cagtttgcgg
    12841 tgaaaaacaa cgaagtctac ctgattgaag ttaacccgcg tgcggcgcgt accgttccgt
    12901 tcgtctccaa agccaccggc gtaccgctgg caaaagtggc ggcgcgtgtg atggctggca
    12961 aatcgctggc tgagcagggc gtaaccaaag aagttatccc gccgtactac tcggtgaaag
    13021 aagtggtgct gccgttcaat aaattcccgg gtgttgaccc gctgttaggg ccagaaatgc
    13081 gctccaccgg ggaagtgatg ggcgtgggcc gcaccttcgc tgaagcgttt gccaaagcgc
    13141 agctgggcag caactccacc atgaagaaac acggtcgtgc gttgctttcc gtgcgcgaag
    13201 gcgataaaga acgcgtggtg gacctggcgg caaaactgct gaaacagggc ttcgagctgg
    13261 atgcgaccca cggcacggcg attgtgctgg gcgaagcggg cattaatccg cgtctggtga
    13321 acaaggtgca tgaaggccgt ccgcacattc aggaccgcat caagaatggc gaatatacct
    13381 acatcatcaa caccacttca ggccgtcgcg cgattgaaga ctcccgcgtg atccgtcgca
    13441 gtgcgctgca atataaagtg cattatgaca ccaccctgaa cggtggtttc gctaccgcga
    13501 tggcgctgaa tgccgacgcg accgaaaaag taatttcggt gcaggaaatg cacgcgcaga
    13561 tcaaataata gcgtgtcatg gcggatattt ttcatccgct aatttgatcg aataagtaat
    13621 acggttctct gatgaggacc gttttttttg cccattaatt aaatcttttg gggaatcgat
    13681 atttttgatg acataagcag gatttagctc acactt
//
LOCUS       DADMKF010000110        13567 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.112, whole genome shotgun sequence.
ACCESSION   DADMKF010000110 DADMKF010000000
VERSION     DADMKF010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13567
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.112"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            <1..1164
                     /locus_tag="JNC24_002916"
     CDS             <1..1164
                     /locus_tag="JNC24_002916"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HAY9159613.1"
                     /translation="INKNWIDKEEYPQSAAIDLRCVNMVADLWHAPAPKNGQAVGTNT
                     IGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCGPVQICWHKFARYWDVELREIP
                     MRPGQLFMDPKRMIEACDENTIGVVPTFGVTYTGNYEFPQPLHDALDKFQADTGIDID
                     MHIDAASGGFLAPFVAPDIVWDFRLPRVKSISASGHKFGLAPLGCGWVIWRDEEALPQ
                     ELVFNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRLGREGYTKVQNASYQVAAYLAD
                     EIAKLGPYEFICTGRPDEGIPAVCFKLKDGEDPGYTLYDLSERLRLRGWQVPAFTLGG
                     EATDIVVMRIMCRRGFEMDFAELLLEDYKASLKYLSDHPKLQGIAQQNSFKHT"
     gene            1534..2358
                     /gene="gadX"
                     /locus_tag="JNC24_002917"
     CDS             1534..2358
                     /gene="gadX"
                     /locus_tag="JNC24_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HAY9159614.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRV
                     DKCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTDRSAEGIRL"
     gene            2726..3454
                     /gene="gadW"
                     /locus_tag="JNC24_002918"
     CDS             2726..3454
                     /gene="gadW"
                     /locus_tag="JNC24_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HAY9159615.1"
                     /translation="MTHVCSVILIRRSFDIYHEQHKISLHNESIVLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(3817..6930)
                     /locus_tag="JNC24_002919"
     CDS             complement(3817..6930)
                     /locus_tag="JNC24_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HAY9159616.1"
                     /translation="MANYFIDRPVFAWVLAIIMMLAGGLAIMNLPVAQYPQIAPPTIT
                     VSATYPGADAQTVEDSVTQVIEQNMNGLDGLMYMSSTSDAAGNASITLTFETGTSPDI
                     AQVQVQNKLQLAMPSLPEAVQQQGISVDKSSSNILMVAAFISDNGSLNQYDIADYVAS
                     NIKDPLSRTAGVGSVQLFGSEYAMRIWLDPQKLNKYNLVPSDVISQIKVQNNQISGGQ
                     LGGMPQAADQQLNASIIVQTRLQTPEEFGKILLKVQQDGSQVLLRDVARVELGAEDYS
                     TVARYNGKPAAGIAIKLATGANALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFIE
                     ISIQEVFKTLVEAIILVFLVMYLFLQNFRATIIPTIAVPVVILGTFAILSAVGFTINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVIAEDKLPPKEATHKSMGQIQRALVGIAVVLS
                     AVFMPMAFMSGATGEIYRQFSITLISSMLLSVFVAMSLTPALCATILKAAPEGGHKPN
                     ALFARFNTLFEKSTQHYTDSTRSLLRCTGRYMVVYLLICAGMAVLFLRTPTSFLPEED
                     QGVFMTTAQLPSGATMVNTTKVLQQVTDYYLTKEKDNVQSVFTVGGFGFSGQGQNNGL
                     AFISLKPWSERVGEENSVTAIIQRAMIALSSINKAVVFPFNLPAVAELGTASGFDMEL
                     LDNGNLGHEKLTQARNELLSLAAQSPDQVTGVRPNGLEDTPMFKVNVNAAKAEAMGVA
                     LSDINQTISTAFGSSYVNDFLNQGRVKKVYVQAGTPFRMLPDNINQWYVRNASGTMAP
                     LSAYSSTEWTYGSPRLERYNGIPSMEILGEAAAGKSTGDAMKFMADLVAKLPAGVGYS
                     WTGLSYQEALSSNQAPALYAISLVVVFLALAALYESWSIPFSVMLVVPLGVVGALLAT
                     DLRGLSNDVYFQVGLLTTIGLSAKNAILIVEFAVEMMQKEGKTPIEAIIEAARMRLRP
                     ILMTSLAFILGVLPLVISHGAGSGAQNAVGTGVMGGMFAATVLAIYFVPVFFVVVEHL
                     FARFKKA"
     gene            complement(6955..8112)
                     /gene="mdtE"
                     /locus_tag="JNC24_002920"
     CDS             complement(6955..8112)
                     /gene="mdtE"
                     /locus_tag="JNC24_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtE"
                     /protein_id="HAY9159617.1"
                     /translation="MNRRRKLLIPLLFCGAMLTACDDKSAENAAAMTPEVGVVTLSPG
                     SVNVLSELPGRTVPYEVAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELN
                     SAKGSLAKALSTASNARITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAV
                     EQATINLQYANVTSPITGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQD
                     FLRMKEEVASGQIKQVQGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIF
                     PNPNGDLLPGMYVTALVDEGSRQNVLLVPQEGVTHNAQGKATALILDKDDVVQLREIE
                     ASKAIGDQWVVTSGLQAGDRVIVSGLQRIRPGIKARAISSSQENASTESKQ"
     gene            8172..8450
                     /locus_tag="JNC24_002921"
     CDS             8172..8450
                     /locus_tag="JNC24_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9159618.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYLGNKVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(8451..8978)
                     /gene="gadE"
                     /locus_tag="JNC24_002922"
     CDS             complement(8451..8978)
                     /gene="gadE"
                     /locus_tag="JNC24_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HAY9159619.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(9777..10349)
                     /gene="hdeD"
                     /locus_tag="JNC24_002923"
     CDS             complement(9777..10349)
                     /gene="hdeD"
                     /locus_tag="JNC24_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HAY9159620.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
     gene            10604..10936
                     /gene="hdeA"
                     /locus_tag="JNC24_002924"
     CDS             10604..10936
                     /gene="hdeA"
                     /locus_tag="JNC24_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HAY9159621.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            11052..11378
                     /gene="hdeB"
                     /locus_tag="JNC24_002925"
     CDS             11052..11378
                     /gene="hdeB"
                     /locus_tag="JNC24_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HAY9159622.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            11442..12089
                     /locus_tag="JNC24_002926"
     CDS             11442..12089
                     /locus_tag="JNC24_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HAY9159623.1"
                     /translation="MTAEFIIRLILAAIACGAIGMERQMRGKGAGLRTHVLIGMGSAL
                     FMIVSKYGFADVLSLDHVGLDPSRIAAQVVTGVGFIGAGNILVRNQNIVGLTTAADIW
                     VTAAIGMVIGSGMYELGIYGSVMTLLVLEVFHQLTFRLMNKNYHLQLTLVNGNMVSML
                     DWFKQQKIKTDLVSLQENEDHEVVAIDIQLHATTSIEDLLRLLKGMAGVKGVSIS"
     gene            complement(12131..12661)
                     /locus_tag="JNC24_002927"
     CDS             complement(12131..12661)
                     /locus_tag="JNC24_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY9159624.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIVFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEANVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(12817..13383)
                     /gene="slp"
                     /locus_tag="JNC24_002928"
     CDS             complement(12817..13383)
                     /gene="slp"
                     /locus_tag="JNC24_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HAY9159625.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
BASE COUNT         3688 a         3072 c         3132 g         3675 t
ORIGIN      
        1 atcaataaaa actggatcga caaagaagaa tatccgcaat ccgcagccat cgacctgcgt
       61 tgcgtaaata tggttgccga tctgtggcat gcgcctgcgc cgaaaaatgg tcaggccgtt
      121 ggcaccaaca ccattggttc ttccgaggcc tgtatgctcg gcgggatggc gatgaaatgg
      181 cgttggcgca agcgtatgga agctgcaggc aaaccaacgg ataaaccaaa cctggtgtgc
      241 ggtccggtac aaatctgctg gcataaattc gcccgctact gggatgtgga gctgcgtgag
      301 atccctatgc gccccggtca gttgtttatg gacccgaaac gcatgattga agcctgtgac
      361 gaaaacacca tcggcgtggt gccgactttc ggcgtgacct acaccggtaa ctatgagttc
      421 ccacaaccgc tgcacgatgc gctggataaa ttccaggccg acaccggtat cgacatcgac
      481 atgcacatcg acgctgccag cggtggcttc ctggcaccgt tcgtcgcccc ggatatcgtc
      541 tgggacttcc gcctgccgcg tgtgaaatcg atcagtgctt caggccataa attcggtctg
      601 gctccgctgg gctgcggctg ggttatctgg cgtgacgaag aagcgctgcc gcaggaactg
      661 gtgttcaacg ttgactacct gggtggtcaa attggtactt ttgccatcaa cttctcccgc
      721 ccggcgggtc aggtaattgc acagtactat gaattcctgc gcctcggtcg tgaaggctat
      781 accaaagtac agaacgcctc ttaccaggtt gccgcttatc tggcggatga aatcgccaaa
      841 ctggggccgt atgagttcat ctgtacgggt cgcccggacg aaggcatccc ggcggtttgc
      901 ttcaaactga aagatggtga agatccggga tacaccctgt acgacctctc tgaacgtctg
      961 cgtctgcgcg gctggcaggt tccggccttc actctcggcg gtgaagccac cgacatcgtg
     1021 gtgatgcgca ttatgtgtcg tcgcggcttc gaaatggact ttgctgaact gttgctggaa
     1081 gactacaaag cctccctgaa atatctcagc gatcacccga aactgcaggg tattgcccag
     1141 cagaacagct ttaaacacac ctgataacat aacgttgtaa aaaccgaatg cccagccttt
     1201 aaaaaaacag ctgggcattc ggttgcttat taatcgcaat aatatattgg ctgtctattc
     1261 atcgtgttga taagatatat gttatatgaa tgtttattaa atgtagcgat tgcctttaat
     1321 gtcacgaaaa gggattattt gcttgctatt aatttcactg cgttcttaaa cacatttttg
     1381 tattataaat ccagtcatcc tgcccgtaga acatcacaca ttatcatcct gttctcccgc
     1441 ttcgtttaaa tttatttatc aatcaatttg acttaagagg gcggcgtgct acattaatta
     1501 acagtaatat gtttatgtaa tattaagtca actatgcaat cattacatgg gaattgtcta
     1561 attgcgtacg caagacataa atatattctc accatggtta atggtgaata tcgctatttt
     1621 aatggcggtg acctggtttt tgcggatgca agccaaattc gagtagataa gtgtgttgaa
     1681 aattttgtat tagtatcaag ggatacgctt tcattatttc tgccgatgct caaggaggag
     1741 gcattaaatc ttcatgcaca taaaaaaatt tcttcattac tcgttcatca ctgtagcaga
     1801 gatattcctg tttttcagga agttgcgcaa ctatcgcaga ataagaatct tcgctatgca
     1861 gaaatgctac gtaaaagagc attaatcttt gcgttgttat ctgtttttct tgaggatgag
     1921 cactttatac cgctgctttt gaacgttttg caaccgaaca tgcgaacacg agtttgtacg
     1981 gttatcaata ataatatcgc ccatgagtgg acactagccc gaatcgccag cgagctgttg
     2041 atgagtccaa gcctgttaaa gaaaaaattg cgcgaagaag agacatcata ttcacagttg
     2101 cttactgagt gtagaatgca acgtgctttg caacttattg ttatacatgg cttttcaatt
     2161 aagcgagtcg cagtgtcctg tggatatcac agcgtgtcgt atttcattta cgtctttcga
     2221 aattattatg ggatgacgcc cacagagtat caggagcgat cggcgcaggg attgccgaac
     2281 cgtgactcgg cggcaagtat tgttgcgcaa ggaaattttt acggcactga ccgttctgcg
     2341 gaaggaataa gattatagaa ttttactcag acataaaaaa aacccggcat aggggaccgg
     2401 gaagaggata gtctgccgtc tccagactaa taaaccgtta taacactccc tgttggcacg
     2461 ggaaactttg tgctctcagt aagttaaata taacttttac tggaaataag atcagccatt
     2521 tttttataaa cataagctat acgctgtgcg aaaatatatt cgtgctgcat ttacttatta
     2581 tcaattaact gttatgcaaa actactttgt ggataaattt tggtcctacc aaatctggca
     2641 gttttttcgc taagaaacag tctggcaaca tttcattagt atactgaaat tgaaataatc
     2701 gcagtatgaa atataaggga taatcatgac tcatgtctgc tcggtgatcc tcattcgtcg
     2761 ttcattcgat atttatcatg aacagcataa aatatcgctg cataacgaga gtatcgtgct
     2821 gctggagaaa aatttggcag acgattttgc gttttgttca ccggatacgc gacgactgga
     2881 tatcgatgag ctgacagttt gccattactt acaaaatatt cgtcagctac cacgcaattt
     2941 agggttacat agcaaagacc gtctattaat taaccagtca ccccccatgc cgctggtgac
     3001 ggcgattttt gatagcttca atgaatccgg ggtaaattca ccgatactga gcaatatgct
     3061 ctacctttcc tgtttatcga tgttttctca taagaaagaa ctgatcccct tacttttcaa
     3121 tagcatcagc actgtttcag gaaaagttga acgccttatt agctttgata tcgccaaacg
     3181 ttggtatctg cgcgatatcg cggaaagaat gtataccagc gagagtctca taaaaaaaaa
     3241 gttgcaggat gaaaatacct gtttcagtaa aatattactc gcttccagga tgtcgatggc
     3301 cagacgatta ctcgagttac gtcaaattcc tctgcatact attgcagaaa aatgtggcta
     3361 tagcagtacg tcgtacttta taaacacatt tcgacaatat tatggtgtaa cgccacatca
     3421 gtttgcgcaa cattcgccag gtaccttttc ctgacatatt ttgcatttga atattggtca
     3481 ggatctcaca cctgcttcat gtgaaactct tccctgatga tttctgccgg gctaccggct
     3541 agttctcttt cgcagtttgc caggcggtat aggtattttc aggaggaaaa gactcggcat
     3601 gtttgggatt attaagctga caattcatac gattacggga atgtgggtca gtattgtgct
     3661 gttcaaattg atgacgaatg gctggagtgg tttctacttt cagtgctgtg tactaagcct
     3721 ggtattcctg acggttagct ggttgttgag tggtgaatgg ttagcaggaa agagtaaggc
     3781 tgaaccttca cgttcaacct tactctcatt tacacgttac gcttttttaa agcgggcaaa
     3841 gagatgttcc actacaacga aaaagaccgg aacgaagtaa attgccagca ctgttgcggc
     3901 aaacatcccg cccatcacgc cggtacctac cgcgttttgc gcgccagaac cggcaccatg
     3961 actgataacc agcggcagca cgccgagaat aaaggcgaga gaggtcatca ggattgggcg
     4021 taaacgcatc cgcgccgctt cgatgattgc ctctatcggc gttttccctt ctttctgcat
     4081 catctcaacg gcaaattcga caatcaggat ggcgttcttg gcggaaagcc cgatggtggt
     4141 cagcaaacca acctggaagt agacgtcatt acttaagccg cgcagatcgg tggccagtaa
     4201 tgcgccaacg acgcctaacg gaacaaccaa catcaccgag aacggaattg accagctctc
     4261 atagagtgcg gcgagggcga ggaacaccac gaccagtgaa atggcataca gtgcaggagc
     4321 ctggtttgag gataacgctt cctgatacga cagtccggtc catgagtagc cgacgcctgc
     4381 cggaagttta gcgaccaggt ctgccataaa tttcatagcg tcaccggtac ttttcccggc
     4441 cgccgcttca cctaaaatct ccattgacgg gatgccgttg tagcgttcca atcgcggtga
     4501 accataggtc cattcggtag acgagtaggc agaaagcggt gccatcgtgc cagaggcgtt
     4561 gcgtacatac cattggttga tgttatccgg caacatacgg aacggcgtgc ctgcctggac
     4621 atacactttt ttcacccgcc cctggttgag gaagtcgttc acgtagctgc tgccgaaggc
     4681 ggtggaaatt gtctggttga tatcagacag cgccacaccc attgcctcag ctttcgcggc
     4741 gttgacgttc actttgaaca tcggcgtatc ttccaggccg ttcgggcgta ccccggtgac
     4801 ctggtcaggc gattgcgctg ccagtgataa cagctcgttt cgtgcctggg ttagtttttc
     4861 gtgccccagg ttaccgttgt ccagcagttc catatcaaaa cctgacgcgg tacccagttc
     4921 agccaccgcg ggtaagttga acgggaagac gacggcttta ttgatactgc ttaacgcaat
     4981 cattgcccgc tgaatgatcg cggtaaccga gttttcctca ccgacacgtt cagaccacgg
     5041 cttgagactg ataaacgcca ggccgttgtt ttgcccctgg ccgctgaagc caaagccgcc
     5101 aacggtaaac accgactgaa cattatcttt ctcttttgta agataataat ccgtcacctg
     5161 ttgcagcact ttcgtggtgt taaccatggt ggcaccggaa ggtaactgcg cggtggtcat
     5221 aaataccccc tgatcctctt ctggtaagaa agaggtcggt gtacgcagga acagcaccgc
     5281 catcccggcg caaatcagca ggtagaccac catgtagcga ccggtacaac gcaacagcga
     5341 gcgggtgcta tcggtatagt gctgagttga tttttcaaac agcgtgttga agcgtgcgaa
     5401 cagggcgtta ggtttgtgac cgccttccgg cgcggctttc agaatggtgg cgcacagggc
     5461 aggggtcagg ctcattgcca caaatactga aagcagcatg gaggagatca gcgtgatgga
     5521 gaactggcgg tagatctccc cggttgcgcc gctcataaag gccatcggca taaacactgc
     5581 ggaaagaaca acagcaatac cgaccagcgc acgttggatc tgccccatcg atttatgcgt
     5641 cgcttccttc ggtggtagct tatcttccgc aatgacacgc tcgacgttct ccaccaccac
     5701 gatggcgtca tccaccagta accctatcgc cagcaccatc ccgaacatag ttaacgtgtt
     5761 gatggtgaaa ccgaccgccg acaagatcgc aaacgtcccg agaataacca ccggtacggc
     5821 aatcgtcggg atgattgtgg cacggaaatt ctgcaaaaac agatacatga ccaggaagac
     5881 gaggatgata gcctcaacca gtgttttaaa aacttcctga atagaaattt cgataaacgg
     5941 cgtggtgtcg taaggataaa ccgtcttcag acttgcgggg aaataggctg ataagcggtt
     6001 cagctcctct ttgactgccc gcgaggtatc cagggcgttt gctccggtag ccagtttgat
     6061 ggcgatcccg gcagcaggtt tgccgttata gcgcgccacg gtggaataat cttccgctcc
     6121 aagttcgacg cgggcgacat cacgcagcag cacttgcgaa ccatcttgct gaactttcaa
     6181 caggattttg ccaaattctt ccggcgtttg cagacgcgtc tgcacaatga tcgaggcgtt
     6241 tagttgctgg tctgccgcct gtggcatgcc acccagttga ccaccggaaa tctggttgtt
     6301 ttgcacctta atctgggaaa taacatcgga aggtaccaga ttgtatttat tgagtttttg
     6361 cgggtccagc cagatacgca tggcgtactc ggaaccaaag agctgtacgc taccaacgcc
     6421 cgcggtacgg cttagcgggt ctttgatatt agacgctaca tagtccgcga tatcgtactg
     6481 gttgaggctg ccgttatcag aaataaacgc cgctaccatc agaatattac tgctcgactt
     6541 atcaacgcta atcccctgct gctgcaccgc ttcaggtaat gaaggcatag cgagttgcag
     6601 tttattttgc acctgaacct gtgcgatatc aggagatgtc ccagtctcga aggtcagagt
     6661 gatagaggca ttacccgccg catcactggt tgaagacatg tacatcaggc catcaagccc
     6721 attcatattt tgctcaatca cctgagtgac cgagtcttct accgtttgcg catcggcacc
     6781 tggataggta gcgctgacgg taatggtcgg tggcgcaatc tgcggatact gcgcaaccgg
     6841 taagttcatg atcgccagac cacctgcaag catcataata atggcaagta cccaggcaaa
     6901 aaccgggcga tcaataaaat agttagccat gaaagtcccc ttaagcctgc aacgttattg
     6961 tttcgattcg gtgctggcgt tttcctggct ggaggaaatt gctcgtgctt tgatacccgg
     7021 acgaatgcgt tgcaaaccgg aaacgatcac ccgatcgcca gcctgcaagc cagaggtgac
     7081 aacccactgg tcgccgatgg ctttgctggc ttcaatttcg cgtagctgca caacatcgtc
     7141 tttatccaga atgagcgccg ttgctttacc ctgggcgttg tgagtgacgc cttcctgcgg
     7201 caccagtaat acattctggc ggctaccttc atccactaat gccgtgacgt acatgccagg
     7261 cagcaagtca ccatttgggt tggggaaaat cgcccgtaac gtcacggagc ccgtggtttc
     7321 atccaccgtc gggtcggaga atttcagtgt gccggtctgg ctgtagcgtt taccattttc
     7381 cagattgagc tgtactggcg tactgccctg aacctgtttg atttgcccac tggcgacctc
     7441 ttctttcatg cgcaagaaat cctgcaccga ctgcgtgaga tcgacataaa tcgggtccag
     7501 acgttgtacg gtaaccagcg aatctgcctg attagcggta acgagtgcgc cgacggtcac
     7561 cgacgatttc ccgctgacgc ccgtaatcgg cgaggtgaca ttcgcgtatt gcagattgat
     7621 cgtcgcctgt tcaacagccg ctttggcgac ggtgacattg gcttctgctt cattcagctg
     7681 ggtgcgcgcg gtgtcgtaat cctgacggct aacgtagttg gtcttcagca acgatgcctg
     7741 gcggttaaag gtgatgcggg cattgctggc ggtagagagc gctttcgcca gcgagccttt
     7801 ggcggagttt agctcggcct gtaaaggtgc aggatcaatc tgatacagcg aatcgccctg
     7861 gttcacttta tcgccttcga taaagttgcg tttaatgata ataccgccca cctggggacg
     7921 tatctcggca acttcataag gaacggttct accgggcaat tcgctcaaca cattgaccga
     7981 accgggggag agtgtgacga caccgacctc aggcgtcatg gcggcggcgt tttccgccga
     8041 tttgtcatcg caggcggtga gcatcgcgcc gcagaataac aacggtatta acagctttct
     8101 tcttctgttc attttagtcc ctgaaaattc ttgagaaata acggtttttt aaattgcgcg
     8161 cgaggagaga tatgagggat ttacagacca gcggcatagt ggggttgtca gccagtaagg
     8221 tgggatacag gcacagtgat cgacatggtg aggtcaacga caaaaaaaat gttgccgttc
     8281 tgccaacagt tcctgccagc attcgggcaa cgaagggttc tactggtgga tacacatacc
     8341 tggggaataa agtcgtttta gcctctcctt tagtaatcac cgggggtaat gaaatcagca
     8401 tctgcatccc tcgtcatgcc agccatcaat ttcagttgct tatgtcctga ctaaaaataa
     8461 gatgtgatac ccagggtgac gatgtcgctc atacgtctta gtttcagctt gtacataatg
     8521 ttgaatttct gaatctttaa tgtttgttgc gaaatattga gcgtcgacgt gatattgctt
     8581 tcatttttat ttttcagaat cagttgcata attttgaact gattatgtga aaaacataat
     8641 tgtgaattct tatggggcaa gtgtttacca taagtaatct caagaagatt tttgatactt
     8701 tctttgcggc taacaaaata aataggcgct ttagctttta gtttactgat gtgataaggt
     8761 gccaaaacaa taattctctc ggcatctaat ttctccagaa atttaatcgc ttcttcatca
     8821 aggatatgat tgtgataggt atcaatgata accttgaagg gtttattgcc tacttttttg
     8881 atctctgaca gggaattgat ttttatcatt tcgtgattat ctttcaactg ccaaaagccc
     8941 tgtaaaagaa aagaatcttt cgtcatgaga aaaatcataa cttgctcctt agccgttatc
     9001 gtttcgaaca tgtcatcctt ttcattattt acatccttgt ccgaatcgtt gttcaatata
     9061 gtaaacgcct ataagaactt tacccttcgt gaactaatac gtctattaac actaatcaat
     9121 acttcagatt tatccatcac gcactccata tattgatcct aagtaagatt cctggttgtt
     9181 atcagcttgt aaatagtaaa caaaaagtta acgtttgcgg attactgccc aagaataagt
     9241 tatcaataac cagtttgtga tctctgaaga atattactaa agttaaaatg taatccgatt
     9301 taaatatcga gtctccttgt ttcgacttaa gctggcaatt ggattgccag ctttctttta
     9361 acgcattgaa attacctgag taaataacgc attcatgtct tcaggttttt atatagcgtc
     9421 tggagacacg gatatttatg caatgtaatc aacgccagtt gcaaaaaaat gaacccggat
     9481 tacttattta accgcccata gcatcaacgc ttacggaact ctttgttcac tttcccacag
     9541 cacttttaaa acattaaacc tgactacgga aaatatcagc catgataatg tttgaaatgg
     9601 ctaatttgcc atggagtgaa aaaaattaga tgaaattcag taggttgaaa taatcactag
     9661 caggtaatta tttcaatgat agtgcgcaat tgatctacaa cactccgtag cggagagagt
     9721 attaatcgga tcatagtcat atcaagtgac tatgatccgg gtgacaaccg ggataattat
     9781 tgctgcttaa cgaacaaact ggcgaagctg aacaggctgg cggcgctaaa tatcagttca
     9841 attcccacca gtgtggaaac cagcgttaca gacaccatcg gcgttgcacc aaggaatatc
     9901 caggcaatga cgatatccag cacaccaata acgagctgta gccagctgcc tttcattgaa
     9961 cgctgacgat accaactcat caggcgaata acccctgcaa cacagaacaa accggcaata
    10021 aatgccgcaa tggcaaaaat gcccagctcc ggtgcgcgga tgaagaaata gccgatcaat
    10081 aaataggcga ctgcgacgag gaaaccggat aataccggcc agaaattatg actgcggttg
    10141 ctgaataacc cgacaataag cgcaatgccc gagcagatta ataatgcacc cactactgtg
    10201 cttaaaatat cgccagagac gaacgggaaa ctgatacaca gcaacccgac gataaacagt
    10261 agcacggcaa taaactggat tgctctgcga tgttttttaa gcatctccag atcaaacttc
    10321 aaaattgttg ccttatctat atataacata gaaccaccct ataaaattaa gaagaaaatc
    10381 ccctgctatc aatctatgcc aaaaacgcgt ctaagaatgt agtcgattta ataaaaattt
    10441 cctaattaca gtatctgatg catctgtaac tcattgtatt gaaataaaaa tatctgattt
    10501 tgatatttta tatcaacatg acatatacag aaaaccaggt tataacctca gtgtcgaaat
    10561 tgattcgtga cggctctttc actttatagt tgaggatatt acgatgaaaa aagtattagg
    10621 cgttattctt ggtggtctgc ttcttctgcc agttgtgagc aatgcagcgg atgcgcaaaa
    10681 agcagctgat aacaaaaaac cggtcaactc ctggacctgt gaagatttcc tggctgtgga
    10741 cgaatccttc cagccaactg cagttggttt tgctgaagcg ctgaacaaca aagataaacc
    10801 agaagatgcg gttttagatg ttcagggtat tgcaaccgta accccagcta tcgttcaggc
    10861 ttgtactcag gataaacaag ccaactttaa agataaagtt aaaggcgaat gggacaaaat
    10921 taagaaagat atgtaattcc gggaatgcgt tacatcgtac ttccttgcat attgaacagg
    10981 ccggaatatc ttctttaaaa gcagctattc ctcctgttca tatataatct ctatattgaa
    11041 tgggttacaa aatgaatatt tcatctctcc gtaaagcgtt tatttttatg ggcgctgtag
    11101 cggctttgtc actggtgaac gcacaatctg cgctggcagc caatgaatcc gctaaagata
    11161 tgacctgcca ggaatttatt gatctgaatc caaaagcaat gaccccggtt gcatggtgga
    11221 tgctgcatga agaaacagta tataaaggtg gcgataccgt tactttaaat gaaaccgatc
    11281 tcactcaaat tcctaaagtg atcgaatact gtaagaaaaa cccgcagaaa aatttgtata
    11341 ccttcaaaaa tcaagcatct aatgacttgc cgaattaatg aggtacaagt aaaaaggagt
    11401 agcaagttga gccatcttgc tgctcctttt tgcattttta tatgacagca gaatttatta
    11461 tacgtcttat actcgcggca attgcctgtg gcgctattgg catggaaagg caaatgcgcg
    11521 gcaaaggagc agggttacgc acacatgtat taattggcat gggaagcgcc ctgtttatga
    11581 ttgtttcgaa atatggtttt gctgacgtgc tgtctttaga tcacgtcgga ctcgacccca
    11641 gccgtattgc tgctcaggtg gtgacgggcg tcgggtttat cggtgcaggt aacattctcg
    11701 ttcgtaacca aaatattgta ggtctgacga cggcagcgga tatctgggtg accgccgcca
    11761 taggtatggt tattggcagc ggtatgtacg aactgggtat ttatggttca gtgatgacct
    11821 tgttggtgct ggaagtcttc catcaattaa ccttccgcct gatgaacaaa aattaccatt
    11881 tacagctgac attagtgaac ggtaatatgg tatcaatgct cgactggttt aagcagcaaa
    11941 aaataaaaac tgatttagtt tcgttgcagg aaaatgaaga tcatgaagtg gtcgccattg
    12001 atatccagct tcatgccaca acgtcgatag aagacctgtt gcgattatta aaaggtatgg
    12061 caggggtgaa aggcgtttca attagctaat ctgtgtggca gcgtagccag actcaccgta
    12121 agcctgaaat tcacaccaga taatcaatat gctgatggcg taccagctca ttgatccgct
    12181 taagattgag gatcatcatg atattgtgtt tatggcagta gaacgttttt agcgatattt
    12241 tcagtttctc cagaatatct tcagttgtca tttgattcgc aatgcagctc agaacctgat
    12301 gttgcttgcg cgaaagtgac acattcgctt caggcacaga gcagtaactg ccattgatga
    12361 gtgcgacaaa atcaatgata gtgatagtcc gcggaacaaa ggtcaccggt actttcccca
    12421 gacagcgttt aatattaaag ggggagaaaa caatgacatg gacaggtttt aatcgttcaa
    12481 tttgagtgag aaaattagaa tgaaatacat tattcattaa tgtatcaata atgacgaagg
    12541 cattctgatg caacattacg tcgatctctt cttcgttctg tatatgaacg acattacctt
    12601 tactcagaat gtttttcatc gcggtgaaga acatcgtatc cctggtaatt ataagaaaca
    12661 taaccgactc ctggtgcgga cattaacttt ataatcaatt tgccaaaggt aattaacagg
    12721 acagggctta taataataat agttagctta ctgtatatta attttatgta atacatgatg
    12781 cagatgctga gaaagcatct gcatctttcg gtggtgttat ttgaccagct caggtgttac
    12841 ctgactcacc gcattggtgt agtaaggcgc accccagccc ggttccggcc agaatgcacc
    12901 atagccgtaa tcccacaggt tataggtggt attaaccact tctctcaaat gccacacctg
    12961 gatgccctgc atattcactt ccaggaagtt atacgggact ttattgataa agccaggttg
    13021 ttcaccctga atggtaccga ggatggtaac aaagtgatta cgatagttca ctgggtcaag
    13081 gaagccgctt tgtctggcga gcagtcggcc ctgatagttg gcttcaatat caggtttcgc
    13141 atagctatcc aacggtaata cagcgatttc taacaacgta tccgttttgc cattgataac
    13201 gttgataacc ttcccaccaa agcgcgcttg ttgaccaaca tataaccccg gctggttatg
    13261 aacagcaaca aaactttttt gaatatcagg ttggttattg cctttgatat tttgcggaat
    13321 tgaactacat gcggcaagca aaaatgaaag gctgaggatg agtgcacctt ttgtcatgtt
    13381 catgttacta tccttatcaa caaatattaa aaccataata gatatttctt tatagttttc
    13441 atctgattct gaggcgatat gcctgatagt aacgtaaacc tgtctgatgt ttatatccct
    13501 tttgtttagt tcaattccat tgattttggg gcgtggcatg agtatccatc gccccataat
    13561 ataatcc
//
LOCUS       DADMKF010000111        13249 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.113, whole genome shotgun sequence.
ACCESSION   DADMKF010000111 DADMKF010000000
VERSION     DADMKF010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13249
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.113"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            <1..781
                     /gene="umuC"
                     /locus_tag="JNC24_002929"
     CDS             <1..781
                     /gene="umuC"
                     /locus_tag="JNC24_002929"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V subunit
                     UmuC"
                     /protein_id="HAY9159626.1"
                     /translation="VDLSNLERQRKLMSALPVDDVWGIGRRISKKLDAMGIKTVLDLA
                     DTDIRFIRKHFNVVLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYTSMRQ
                     AICSYAARAAEKLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDII
                     NAATRSLDAIWQAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMTVMDTLN
                     AKEGRGTLYFAGQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(827..1357)
                     /gene="dsbB"
                     /locus_tag="JNC24_002930"
     CDS             complement(827..1357)
                     /gene="dsbB"
                     /locus_tag="JNC24_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HAY9159627.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGLEMPQWLLGIFIAYLIVAVLV
                     MISQPFKAKKRDLFGR"
     gene            complement(1503..3044)
                     /gene="nhaB"
                     /locus_tag="JNC24_002931"
     CDS             complement(1503..3044)
                     /gene="nhaB"
                     /locus_tag="JNC24_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HAY9159628.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAIFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYKVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     IGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            3265..3984
                     /gene="fadR"
                     /locus_tag="JNC24_002932"
     CDS             3265..3984
                     /gene="fadR"
                     /locus_tag="JNC24_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HAY9159629.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(4036..5568)
                     /locus_tag="JNC24_002933"
     CDS             complement(4036..5568)
                     /locus_tag="JNC24_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HAY9159630.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNYLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGWATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREMRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSIELLERCPPRMG
                     NL"
     gene            5898..7196
                     /locus_tag="JNC24_002934"
     CDS             5898..7196
                     /locus_tag="JNC24_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HAY9159631.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVEPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKCGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            7206..8263
                     /gene="dadX"
                     /locus_tag="JNC24_002935"
                     /pseudo
     CDS             7206..8263
                     /gene="dadX"
                     /locus_tag="JNC24_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309712.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
     gene            complement(8650..10386)
                     /locus_tag="JNC24_002936"
     CDS             complement(8650..10386)
                     /locus_tag="JNC24_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HAY9159632.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(10481..11395)
                     /gene="ldcA"
                     /locus_tag="JNC24_002937"
     CDS             complement(10481..11395)
                     /gene="ldcA"
                     /locus_tag="JNC24_002937"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HAY9159633.1"
                     /translation="MSLFHLIAPSGYCIKQHAALRGIQRLTDEGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAHGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQ
                     GEGPTCRAEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRDERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGTQLTISGHPVLKM"
     gene            11495..12106
                     /gene="emtA"
                     /locus_tag="JNC24_002938"
     CDS             11495..12106
                     /gene="emtA"
                     /locus_tag="JNC24_002938"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HAY9159634.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLEHVARNHPAPQAPRYIYKLEQALDAM"
     gene            complement(12108..12842)
                     /locus_tag="JNC24_002939"
     CDS             complement(12108..12842)
                     /locus_tag="JNC24_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HAY9159635.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMGQWGVFHFDAQLISISERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            13043..>13249
                     /locus_tag="JNC24_002940"
     CDS             13043..>13249
                     /locus_tag="JNC24_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAY9159636.1"
                     /translation="MGIIAWIIFGLIAGIIAKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIGGSISGFNLHSFLVAVV"
BASE COUNT         3364 a         3366 c         3484 g         3035 t
ORIGIN      
        1 ggtggattta tcaaatctgg aacgccagcg taaattaatg tctgctctcc ccgtggatga
       61 cgtctggggg attggacggc ggatcagcaa aaaactggac gcgatgggga tcaaaaccgt
      121 tcttgatttg gcggatacag atatccggtt tatccgtaaa cattttaatg tcgtgctcga
      181 aagaacggtg cgtgaactgc gcggtgaacc ctgtttgcaa ctggaagaat ttgcaccgac
      241 aaagcaggaa attatctgtt cccgctcgtt tggtgaacgt atcacggatt atacgtcgat
      301 gcggcaggcc atttgtagtt acgctgcccg ggcggcggaa aaacttcgca gtgagcatca
      361 atattgtcgg tttatctcca catttattaa gacgtcacca tttgcgctca atgaacctta
      421 ttacggcaat agcgcgtcgg taaaactgct gacgcccact caggacagca gggatatcat
      481 taacgccgct acgcgatctc tggatgccat ctggcaagcg ggccatcgct accaaaaagc
      541 gggcgtgatg ctgggggatt tcttcagtca gggagtcgcg cagctcaatt tattcgatga
      601 caacgcaccg cgccccggga gtgagcaatt gatgacggta atggatacac tgaatgctaa
      661 agagggcaga ggaacactct attttgccgg gcaggggatc caacaacaat ggcagatgaa
      721 gcgagccatg ctttcaccac gttatacaac gcgaagttct gatttactgc gggtcaaata
      781 aatatagcgg caggaaaaaa gcgctcccgc aggagcgccg aatggattag cgaccgaaca
      841 gatcacgttt tttcgcttta aacggctggg aaatcatcac cagcaccgcg acaatcaggt
      901 aagcgataaa aataccgagc agccactgcg gcatttccag acctaaaaaa tcccactgac
      961 gctcggcgca atcgccagag gcgacaaaca cttgcggcac ccatttatcc agcggcagcc
     1021 attccgggaa acgaaccata aaatcacagg tggcaaacgg cgaaggatag agctgaagca
     1081 tggtgtgctc gtaagttaac tgcacaccgc ggaacgcact atacaaccag ataaccatcg
     1141 ctacataacg cagcggagtt ttcggggcga tcgcgccaat cagcgcagca cccagaacgc
     1201 cgaataacgc gcagcgttca taaatacaga gcacgcaagg tttcagtaac atcacatgct
     1261 ggaaccacag cgccgtcagt tccagtgcca gagcagtaaa cgccatcaac agccacgcgc
     1321 cccggccttg tgaacattgg ttcaaaaatc gcaacataat catttccctg caatatgcat
     1381 agagtgcgca gtttaaacca attcattcgc tgcgccacca ggaggcagca aaaataggac
     1441 gcaattggac aattatcggg cgaaaaggca aaccgggcat ttatatgccc ggtaagttgt
     1501 tatcaaagcg ttgctatcca gcccatttgc ataaaccatt cggttacagg ggcaagcgta
     1561 aactcgacgc agagcagtcc gacgagtgtc aggacgaggg tgtaaggcag ggccatccac
     1621 accatgcggc cataagagag gcgaatcaat ggcgcgagtg cagaggtcag caggaatagg
     1681 aacgcagcct gaccgttcgg cgtagcgaca gagggcagat tggtaccggt attaatggcg
     1741 accgccagca gctcgtattg cttcaacgtg atagcgccac tttccattgc cgctttcgct
     1801 tcgttgatat aaatcgtccc cacgaagacg ttatccgaaa tggatgacag cagaccgttg
     1861 aatatataga acagcgacag ctgagcatgt tccgatgcct gcaacacaaa ctgaataatc
     1921 ggcgaaaaca gttgttggtc gataatcacc gcgactaccg agaaaaacac cgtcaacagt
     1981 gcggtgaatg gcagagattc ggtgaaggct ttaccgatag catgctcatc ggtgacaccg
     2041 atcaatgatg tcgccagaat aatgactgac aaaccaatca aaccaacttc cgccagatgc
     2101 aacgccagcg cagtcaccaa ccagacgcca ataatcgcct ggacaatcag acggatttta
     2161 tcctgacggg tacgctggtg gcggctttga tcgtcaaact gctgcagcac ttcgcggact
     2221 ttctccggca gcgtttcacc gtagccaaac caacgcagct tctccaccag caggcaggtt
     2281 aacaggccac aaatcagaac cggaacggtc accggcgaca tgcgcaggaa gaaatcgcca
     2341 aaatgccagc cagccgcttt agcgatgatc aggttctgtg gttcgcccac catggtcatc
     2401 acgccgccta atgcggtgcc gacaccggca tgcatcatca ggctacgcag aaagccacgg
     2461 aactgttcca gaaccacttt gtaatgcttg tcgatatgac tatcgtcttg caggtcggta
     2521 tcttcagtac gggaagaggc tacgcggtga taaataccat aaaaaccgac tgcaacgctg
     2581 atcaccaccg ccacgacggt taaggcatcg aggaacgcgg agaggaacgc cgccgccacg
     2641 caaaaagaga gcgacagcag cattttggag cgaatgctta acagcaaacg ggtaaatatg
     2701 aacagcaata gctgtttcat aaaatagata cccgccacca taaacatcag taacagcaag
     2761 acttcaagat ttgccgccac ctcttcacgg acgtgttccg cgctggtcat gccgatgaat
     2821 atcgcttcga tagccaacag accaccgggg agcagcgggt agcatttcag ggccatcgcc
     2881 agagtgaaaa taaattccgc taccagcaac cagcccgcga cgaaagggct gatgaggaaa
     2941 attaacgggt ttacgattaa gaaaatgatg agggcgagtt tgtaccagtc gggggactgg
     3001 cccaaaaaat tgcgccatag cgcgcggccc caggagatct ccatgatggt ttcccttacc
     3061 ttacaaataa tcaatgatgt ttttatgttt aaacgcaaag cttaacggtc aggcaggagt
     3121 gaggcaagtc ttgatagtca aggggaaaga gatgcggaaa atgaagcctt gatccctttt
     3181 tcttcttttt gtctgctatc agcgtagtta gccctctggt atgatgagtc caactttgtt
     3241 ttgctgtgtt atggaaatct cactatggtc attaaggcgc aaagcccggc gggtttcgcg
     3301 gaagagtaca ttattgaaag tatctggaat aaccgcttcc ctcccgggac tattttgccc
     3361 gcagaacgtg aactttcaga attaattggc gtaacgcgta ctacgttacg tgaagtgtta
     3421 cagcgtctgg cacgagatgg ctggttgacc attcaacatg gcaagccgac gaaggtgaat
     3481 aatttctggg aaacttccgg tttaaatatc cttgaaacac tggcgcgact ggatcacgaa
     3541 agtgtgccgc agcttattga taatttgctg tcggtgcgta ccaatatttc cactattttt
     3601 attcgcaccg cgtttcgtca gcatcccgat aaagcgcagg aagtgctggc taccgctaat
     3661 gaagtggccg atcacgccga tgcctttgcc gagctggatt acaacatatt ccgcggcctg
     3721 gcgtttgctt ccggcaaccc gatttacggt ctgatcctta acgggatgaa agggctgtat
     3781 acgcgcattg gtcgtcacta tttcgccaat ccggaagcgc gcagtctggc gctgggcttc
     3841 taccacaaac tgtcggcgtt gtgcagtgaa ggcgcgcacg atcaggtgta cgaaacagtg
     3901 cgtcgctatg ggcatgagag tggcgagatt tggcaccgga tgcagaaaaa tctgccgggt
     3961 gatttagcca ttcaggggcg ataatccctt ccgtttaaag agcaaacccc tcaaacgggg
     4021 ggttttttgt tgtttttaca gatttcccat tcttggcggg caacgttcca gcaactcgat
     4081 gctgccgtct tcgttttgct gttcgagcat cacatcaaat ccccacaggc gatgcacatg
     4141 cttcaggact tctttgcgcc cccgatccag cggtgcgcga ttatgtggaa tataacgcag
     4201 cgtcagcgaa cggtcgccgc gcaaatccac gttccagatc tgaatattcg gctccagatt
     4261 acttaagtta tattgcgacg ataaccggtt acgcatctcc cgataacctt cttcattatg
     4321 aatagcggaa atctccagat aattatgccg atcatcatcc agcacggtga agaagcggaa
     4381 atcacgcatc actttcggtg acagaaactg gctgataaaa ctttcatctt tgaaatcacg
     4441 catcgcgaaa tgcagcgttt ccagccagtc ggaaccggcg atatctggga accagtattt
     4501 gtcttcttcc gttggcgact gacaaatccg tttaatatcc tggaacatgg cgaacccgag
     4561 ggcatacggg ttgatgccgc tgtaccacgg gctgttatag gggggctgga agaccacatt
     4621 ggtgtggctg tgcaaaaact ccagcataaa acgttccgtt actttccctt catcatacag
     4681 atggttaagg atggtgtagt gccagaaggt cgcccagcct tcgttcatca cctgagtctg
     4741 tttttgcgga taaaaatact ggctcacctt acgcacaata cgcaggattt cacgctgcca
     4801 tgattccagc agtggcgcat ttttctccat aaaatagagc aggttttctt gtggttcgga
     4861 cggatagcgg cgcgcttcag caaccgtttt ctcttcctcg cgcttcggca gagtacgcca
     4921 gagcatattg acctgacttt gcagatactc ttcgcgactt ttctgccggg ctttctcttc
     4981 ttgcagcgag attttttgcg ggcgtttgta ccggtccacg ccgtagttca tcagcgcgtg
     5041 gcacgagtcc agaagccgtt ctacttcatc cacgccataa cgttcttcgc actcggtaat
     5101 atatttacgg gcaaaaatca gataatcgac aatcgaactg gcgtcggtcc aactgcggaa
     5161 taagtaattg tttttaaaga aagagttatg cccatagcag gcgtgtgcca tcaccagcgc
     5221 ttgcatggta atggtgttct cttccatcag gtaagcgata cacgggttag agttaatgac
     5281 gatttcatag gccagccctt gctgaccgtg cttatacagc cgttcagtct cgataaattt
     5341 tttaccgaat gaccagtgcg gatagttaat tggcatgccg acgctggagt aggcatccat
     5401 catctgttct gaggttatca cttcaatctg gtgcgggtag gtatccagcc ggtagagttt
     5461 cgccacccgg tctatctctg ccagataaac atccagcagg tcgaacgtcc agtcgggtcc
     5521 atcgctcaaa cgtgtggtgt ccttattcat agaatcgatc gtcgccatac gcgcacctca
     5581 ttgttgtcgg cgctctctgt gtggagcacc tcatttcaag catagaacac ctgttaaaaa
     5641 ccgcgtcgcc ggagaatttt tttctttgcg atttcttatt atcagagtgc cactaatccg
     5701 cttctgaacg gaattttatg ctggataaaa agggcgttca gcaggagata ctaaagacgc
     5761 catattgccg cagagtcagg gagatgtgag ccagctcacc ataaaaaagc cgtatgttga
     5821 ataatgtttt caactgagtt atcaagatgt aattagatta ttattctttt actgtatcta
     5881 ccgttatcgg agtggctatg cgagttgtca tactgggaag tggtgtggta ggcgttgcca
     5941 gcgcctggta cttaaatcag gcaggacatg aggtcaccgt cattgatcgg gagccggggg
     6001 ccgccctgga aaccagcgcc gcgaatgccg ggcaaatctc ccccggatat gctgcaccgt
     6061 gggcggcacc aggtgtgcct ttaaaagcga tcaaatggat gttccagcgc catgcgccgc
     6121 tggcggttcg tctcgacggt acgcagtttc agttgaaatg gatgtggcaa atgttacgta
     6181 actgcgacac cagccactac atggaaaaca aagggcggat ggtgcgtctg gcggaataca
     6241 gccgtgattg cctgaaagca ttgcgcgccg aaaccaatat tcagtatgaa gggcgtcagg
     6301 gtgggacgct gcaactgttc cgtaccgaac aacagtatga aaatgcgacc cgcgatatcg
     6361 ccgtgctgga agatgccggc gtaccgtatc agctgctgga atccagccgc ctggcggaag
     6421 tggagcccgc gctggcagaa gtagcgcaca aactgacggg tggtctgcaa ctgcccaatg
     6481 atgaaaccgg agactgccag ctgtttaccc agaatctggc gcggatggca gagcaggcgg
     6541 gggttaaatt ccgctttaat acgcccgttg accaactgct ttgcgacggc gagcaaatct
     6601 acggcgtgaa gtgtggcgat gaagtgatta aggccgatgc gtatgtgatg gcgtttggtt
     6661 cttactcgac ggcgatgctc aaaggcattg ttgatattcc ggtgtatccg ctgaaaggct
     6721 actcgctgac tattccaatt gcgcaggaag atggtgcgcc agtatccacc attcttgatg
     6781 aaacctacaa aatcgccatt acccgtttcg ataaccgcat tcgtgttggc ggaatggcgg
     6841 aaattgttgg ttttaacacc gagctgttgc aaccgcgtcg tgaaacgctg gaaatggtgg
     6901 ttcgcgatct ctatccacgc ggcggtcatg tcgagcaggc gactttctgg acggggttgc
     6961 gcccgatgac gccagacggt acgccggttg tcgggcgtac acgctttaaa aatctgtggc
     7021 tgaataccgg tcacggcacg ctcggctgga cgatggcttg cggttccggt cagttgttaa
     7081 gcgatctgct ctctggtcgc acgccagcga tcccatatga ggatctaagc gtagcgcgct
     7141 acagccgtgg atttacgcca tcacgtccgg gccatttaca tggcgcacac agctaaggaa
     7201 acgagatgac ccgtccgata caggccagcc tcgatctgca ggcattaaaa cagaatctgt
     7261 ccattgtccg ccaggccgcg ccgcacgcgc gcgtctggtc ggtggtaaaa gcgaacgctt
     7321 acgggcatgg tattgagcgt atctggagcg cgctcggggc caccgatggc tttgcattac
     7381 ttaacctgga agaggcaata acgttacgtg agcgcggctg gaaggggccg atcctgatgc
     7441 tggaaggatt tttccatgct caggatctgg agatttatga ccagcaccgc ctgaccacct
     7501 gcgtacacag caactggcag ctcaaagcac tgcaaaatgc gcggctaaaa gcaccgttgg
     7561 atatttatct taaagtgaac agtgggatga atcggttggg cttccagccc gatcgcgtgc
     7621 ttaccgtctg gcagcagttg cgggcaatgg cgaatgttgg cgagatgacc ctgatgtcgc
     7681 attttgccga agcggaacat cctgatggaa tttccagcgc gatggcgcgt attgagcagg
     7741 cggcggaagg gctggagtgt cggcgttcgt tgtccaattc ggcggcgact ctgtggcacc
     7801 cggaagcgca ttttgactgg gttcggcctg gcattatttt gtatggcgct tcgccgtccg
     7861 gtcagtggcg tgatatcgcc aataccggat tacgtccggt gatgacgcta agcagtgaga
     7921 ttattggtgt ccagacgcta aaagcgggtg agcgtgtggg ctacggcggt cgctatactg
     7981 cgcgcgatga acagcgaatc ggcattgtcg ccgcagggta cgccgacggt tatccgcgcc
     8041 acgcgcctac cggtacccct gttttagtgg acggcgtgcg caccatgacg gtggggaccg
     8101 tctcgatgga tatgctagcg gtcgatttaa cgccttgccc gcaggcgggt attggttgtg
     8161 gggcaaggag atcaaaattg atgatgtcgc cgccgctgcc ggaacggtgg gctatgagtt
     8221 gatgtgcgcg ctggtgctac gcgtcccggt tgtgacggtg taacttgttg taagccggat
     8281 cggaggcaac gtcttctggg tgcaaaaaaa atcatccatc cggctggtca gcaactgtag
     8341 ttgttaatgt gacagagcca ttgcccatga tagtgtccat taaaaggatg gacactattt
     8401 ccccggaacc tgaactcacc gcacaggcgt tctacataaa acgcttacgc ttcattgttg
     8461 actcgaactc gacttcagat aaatcacgct ttacccttga tggagcctgt acatagattt
     8521 gtgtaattgc ctgattttga tatgttcaat tcaacatcaa atgaaggtta aattatggac
     8581 gacaaacaat tgcaggctca ggctgcgttc agcaaagcat cgcaaccggc gatagatgct
     8641 tcattaaatt taagattcag cttcctcttc agccacccgt acgccaatct tcaacacttc
     8701 attatcttct ttctcggcca ccgtccagat catcccggca aactctacct ggtcaccgac
     8761 aaccggtgcc gcgcctaaca actgctggac aatttcaccc agcgtttgct gcttatcacg
     8821 atattctcgc ccgtcttcca ggccatatat cagcgcaaca tcagcatatt tggcgctggc
     8881 ttcgagaatg aagtcaccaa agaagcgttg atccagcgcg accggtggcg actggctgaa
     8941 cagtttaccg agcgccggga gatcgcgttc ccgaccaatt acacacaaca catcgccttc
     9001 gcgcagtcgg gtgctgccgg taggatgaag caactggtta tcacgaaata gtgccgcaat
     9061 acgcgtctct tttggcatat gcagatcgcg cagtgccgca cctacacacc atttatcggc
     9121 actcaattga taaacaaact gctcccacgg attttccgga tgaatatcca gaccaacgcg
     9181 tgacactgga cgtccgacgg gcggaaccac cactttggct tttttcgccg cccacgagag
     9241 tgatgttccc tgcaagagca gtgaaaccag aaccacaaag aaggcgacat taaagaacag
     9301 acgtgcattc tccagccccg ccatcatcgg gaacacggcc aggatgattg gcaccgcgcc
     9361 gcgtaatcct acccagctga taaacacgcg ctcacgcaga ttgaaaccgc ggaaggggag
     9421 caatccagca aataccgaaa ggggacgggc gaagaatatc atccatgccg acaaaatgag
     9481 cgccggaatg gcaataggca gcagatcgct tgggttaacc aatagcccca gcaccaggaa
     9541 catggcgatt tgcgccagcc aggcgaggcc gtcgaaattt tgcaggatgc cgtagcggtt
     9601 gcgaatcggc cgattaccca gcagaaaacc gcacagataa acagccagaa taccgctgcc
     9661 ttccagcgcc gtggttaaag caaaaattaa aataccgccg cttaatgcca gcaatggata
     9721 taatccggca ggcagggcga tgcgattaat catttgcagc agtaaataac caccgccaag
     9781 tccgatgaca atcccgaggc caaattgctg cagaatatcg accacgaaca tccaactgac
     9841 actgctttca tgttgctgga tcatcgcaat cagggtaatc gtcagaaaga ccgccattgg
     9901 atcattactg ccggattcaa tttccagcgt cgagccaaca cgttcgttaa gccccttacc
     9961 acccagcaga gaaaagaccg ctgcagcatc ggtagagccg acgatagcgc cgattaataa
    10021 gccttcaatc aaatcaagat taaacagcca cgccgccatc atgccggtta aaccagaggt
    10081 gataagcacg cccagcgtcg ccagcgacag tgccggtcct aacgccacac gaaaggaact
    10141 ggcctgagtg cgcatcccgc cgtcgagcaa aataatcgcc agtgccaggt tacttaccat
    10201 gtaggcgaag gggtaattat caaacgggat accgccgacg ccatcgactc ctgccagcat
    10261 gccgatcgcc agaaaaataa ccagaatagg aatgccaaga cgggaagaaa atgaactaag
    10321 taatatactg ctggtgacaa ggatggatcc taagatgaaa aggctaatta ttgttgtggc
    10381 atccaacggt cggttactcc tgattacgct gtctcttata taaaccctac catattagcg
    10441 gcagagacag cgttttactt agccctgaag cgtgattttt ttacatttta agaacaggat
    10501 gaccgctaat cgtcaactga gtgccttccc gggtgttatt cagaatggca tgtgcgccca
    10561 acggcagcgt aaccgtgcgt tgttcatgac caaaatcgag gccggtaatg agtggaatcg
    10621 acaggcggga acgcaaaaac gcgtacactg actccaggtt gtaacctgcg tcataatcat
    10681 tgggcgtgct gccgctaaag ctaccgagaa tgatcgcctt ctgacgtggc aaaattcccg
    10741 catgatagag ctgcaacagc atacgttcgt cccggaaagg gtgctcgtta atatcttcca
    10801 gcaccagaat accgttctca atttttggca tccacggtgt accgatcagt gaaatcagca
    10861 tcgcaagatt gcctccccac aacgtgcctt cggcccgaca tgtcgggcct tccccttgcc
    10921 attcaatggt gaaggtttca ttgcgtaacg ccagccagaa atggtgctcg gtaaaggcgt
    10981 tcagttcatc cgcgccaaag tttgctacca gcatggggcc gctaaaggtg atgacattgc
    11041 catgcgccag aagaccgcac tgaatggcgg taaaatcgct atgcccgcaa atgagcaacg
    11101 ggtcatgttg ttggcgagcc accagtgcct gccagtcaat atctgccagt aaacgactgg
    11161 caccgtaacc gccgcgtaca gccagcacga tggtgttggg ggtggtcagt ctggcaaggg
    11221 aattgagatc ttccaggcgc tccgtttccg tacccgcaaa acgctcacaa cgacgggcaa
    11281 taacctcgac gttattgacc tgatgcccct cgtcggtaag gcgctggata ccacgcagcg
    11341 cggcgtgctg tttaatgcag taacccgatg gggcaattaa gtgaaacaga gacatggcaa
    11401 ttccttgctg acaacagaaa cgaaatgtat atcatgccgc ttaggtgtgc cgttgtcacc
    11461 tcaacggcga ttccaggcta taaggataga agaagtgaaa ttgagatggt ttgccttttt
    11521 gattgtgtta ttagcgggtt gttcatcaaa gcatgactat acgaacccgc cgtggaacgc
    11581 gaaagttccg gtgcaacgtg cgatgcagtg gatgccaata agccagaaag ccggtgcagc
    11641 ctggggcgtc gatccacaat tgatcacggc gattatcgct atcgaatcgg gtggtaatcc
    11701 gaatgcggtg agtaaatcga atgccattgg tttgatgcag ttaaaagctt caacctccgg
    11761 acgtgatgtt tatcgccgta tgggctggag tggtgagccg acgaccagcg agctgaaaaa
    11821 tccggagcgt aatatttcaa tgggggcggc ttacctgaat attctggaaa ccggcccgct
    11881 ggcaggcatt gaagatccga aggtactgca atatgcgctg gtggtgtcat acgctaacgg
    11941 ggcaggtgcg ctgctacgga ctttctcgtc agatcggaaa aaggcgatca gcaaaatcaa
    12001 cgatttggat gctgacgagt tcctcgaaca cgtagcgcga aatcaccctg cgccgcaggc
    12061 tccgcgctat atctacaaac ttgagcaggc actggacgcg atgtaaatca gtcgcgcact
    12121 ttgtccgctt tttcccgggc ttctcgctcg agagagaaaa taatccgctg taattgccgc
    12181 tccaccgtcg ggctgacgtt aagaaaacgg aagctcagac ggggagtggt gatggtttca
    12241 ttcttgccat caatcacttt gcgctcgctg atagagatta actgggcgtc aaagtgaaaa
    12301 acaccccatt gccccatgtt gacttcaatc tgagcgaagc gcatgccttc ttgtaattcg
    12361 gcaggctttg ctgtttccag taatgcgccc atgccgccta acgacaaatc atacaggcgg
    12421 aaacgtaacg tactgttatc cgccagtttg gtctggcaaa aataaggcgg atggagtggg
    12481 gcggagatgc ggaaatatcg gcgtcgttgt acaaaccata aggtgggagg cggtacggtg
    12541 ataaatgccg gaagctgcaa gtattcactc tgctgtagtt gttcaacagt aaactcgact
    12601 ttcgcaccct gagtttcggc ggtaatggta atgtgctgtg cctttagcac agcgatgttg
    12661 tcttcggctt gactgccgaa atccagcacc agtttatccg gggttattgc cagtattttg
    12721 ctgatcagct gcccgccatt ccaactgaga cgcaaaggaa ttgcggcttt gtgcaaatcg
    12781 cgtaacacgc ccaggacggc taacggattt tgtttcagga actgctcatg gtaatgactc
    12841 acgcggaaaa actcctgact gacaaactgt ctttggttta tcggtcacag ttaacaaaac
    12901 ttaatacaaa tgcgtgaata ttttttacat gttgttctta aatcagccga catacgccca
    12961 gcatcgatcc cgcccttgcc tatacttaga gcgttgacgt aagcatttct tgcgtccgat
    13021 tcatcgaaac gagggcctga acatgggaat tattgcttgg attatttttg gcctgatagc
    13081 cggcattatc gccaagctaa tcatgccggg gcgtgatggt ggtggatttt tcctgacctg
    13141 tattctcggg atagtcggtg cggtggtcgg cggctggctg gcgaccatgt ttggcattgg
    13201 cggctccatc agcggtttta atttgcacag cttcctggtg gcggtggtg
//
LOCUS       DADMKF010000112        13121 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.114, whole genome shotgun sequence.
ACCESSION   DADMKF010000112 DADMKF010000000
VERSION     DADMKF010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13121
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.114"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            149..448
                     /locus_tag="JNC24_002941"
     CDS             149..448
                     /locus_tag="JNC24_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9159637.1"
                     /translation="MGNGNFWQLFLGKEPYSKKSDFVKAWLLWWAITILVLFILSLTT
                     ILNLRSIGIGLSFGFCQILGRYTMSLGWSKYWGVLGVFPVTNIVLFLILIFFKKR"
     gene            complement(495..1382)
                     /gene="rarD"
                     /locus_tag="JNC24_002942"
     CDS             complement(495..1382)
                     /gene="rarD"
                     /locus_tag="JNC24_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HAY9159638.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFAFI
                     WVALAIFVMDAIYTQRRKS"
     gene            complement(1434..1901)
                     /locus_tag="JNC24_002943"
     CDS             complement(1434..1901)
                     /locus_tag="JNC24_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001350877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HAY9159639.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            2066..2935
                     /gene="pldA"
                     /locus_tag="JNC24_002944"
     CDS             2066..2935
                     /gene="pldA"
                     /locus_tag="JNC24_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HAY9159640.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYCFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            3068..4897
                     /gene="recQ"
                     /locus_tag="JNC24_002945"
     CDS             3068..4897
                     /gene="recQ"
                     /locus_tag="JNC24_002945"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HAY9159641.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            4961..5578
                     /gene="rhtC"
                     /locus_tag="JNC24_002946"
     CDS             4961..5578
                     /gene="rhtC"
                     /locus_tag="JNC24_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HAY9159642.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLMVGGGLYLCWMGYQMLRGALKKEAVSAPA
                     PQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTARWGIFALIIVETL
                     AWFIVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(5640..6260)
                     /gene="rhtB"
                     /locus_tag="JNC24_002947"
     CDS             complement(5640..6260)
                     /gene="rhtB"
                     /locus_tag="JNC24_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HAY9159643.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            6371..7393
                     /gene="pldB"
                     /locus_tag="JNC24_002948"
     CDS             6371..7393
                     /gene="pldB"
                     /locus_tag="JNC24_002948"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HAY9159644.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            7401..8201
                     /gene="yigL"
                     /locus_tag="JNC24_002949"
     CDS             7401..8201
                     /gene="yigL"
                     /locus_tag="JNC24_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HAY9159645.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNLDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            8277..9176
                     /gene="bioP"
                     /locus_tag="JNC24_002950"
     CDS             8277..9176
                     /gene="bioP"
                     /locus_tag="JNC24_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HAY9159646.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRGNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(9064..10017)
                     /gene="metR"
                     /locus_tag="JNC24_002951"
     CDS             complement(9064..10017)
                     /gene="metR"
                     /locus_tag="JNC24_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HAY9159647.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     ITEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            10253..12514
                     /gene="metE"
                     /locus_tag="JNC24_002952"
     CDS             10253..12514
                     /gene="metE"
                     /locus_tag="JNC24_002952"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HAY9159648.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLTVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALGHNVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVAEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIRQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDVSRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(12799..13044)
                     /locus_tag="JNC24_002953"
     CDS             complement(12799..13044)
                     /locus_tag="JNC24_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAY9159649.1"
                     /translation="MITETEINVLKIMAEKDINWNWMNLDRTLSIRQIPGFGNVVNIV
                     NKLANEGLVIIEDSGHKSMPHYHVTEKGYNFVKSENK"
BASE COUNT         3110 a         3254 c         3493 g         3264 t
ORIGIN      
        1 atatatgcaa tatagcagtc tttgcattga taactataca gtagtaattt agagtgtgta
       61 agtttttatt gatggggtat attttaatta aatataattg aaatgtattt ttattctata
      121 aacaatatct tgggtgaatg gattctatat gggaaatggt aacttttggc agttgttctt
      181 gggtaaagaa ccttacagca aaaaatctga ttttgttaag gcatggttat tgtggtgggc
      241 tattaccatc cttgttctat ttattttaag cctgactacg atattaaatt taagaagtat
      301 aggtataggg ttaagttttg ggttttgtca aatccttggt agatatacga tgtctctagg
      361 atggagtaag tactggggag ttttgggggt atttcctgta acaaatattg ttctttttct
      421 tatcttaatt tttttcaaaa aaagataaat aagtcagtgg gtaggctctt taaacggcct
      481 acccatgtta attcttaact ttttctacgc tgcgtataaa tcgcatccat cacaaaaatt
      541 gccagcgcca cccaaataaa ggcgaaagtc accatcttat cggcacccgg tttttcacca
      601 taaaacgtca cagccagcag gaacatcagc gtcgggccaa tgtactggaa aaagcctaac
      661 gttgagagac gcaagcgcgt ggcagcggcg gtaaaacaca acagcggtac ggtagtgaca
      721 ataccggcgg cgatcagcag taaattcagc gacatcgggt tttgccccat atggctggtt
      781 gagctgtcgg caatagcaaa caggtaaatt gccgccacgg gcagcagcca catggtttcg
      841 attaacatgc cggtttgcgc ttcaacggca atcttcttgc gtaccagacc gtagaaggca
      901 aaactaaatg ccagtcccag cgcgataata ggtagcgaac caaaagtcca cagctggact
      961 aacacgccac atatcgccag aatcaccgcc agccattgca tccggcggaa tcgctcgccg
     1021 aggaaaatca tccccagcac aatgttcacc agcgggttaa taaagtaacc aaggctcgct
     1081 tccagcatat ggtgattgtt caccgcccag ataaacagta gccagttgcc accaatcagc
     1141 acggcagaga ctgccagcat aaaaattttc tgtggcgtct gaatcagcgt ttttaaatag
     1201 gaccactggc ggcaaatgct catcagcacc accataaaga aaaacgacca gatcacgcga
     1261 tgcgtcagga tttcatcggc gggcacgtag taaatcaact tgaagtacgc tggcgctata
     1321 ccccaaataa aataagcggc aagagcgagt aatacgccct gccgcgtttg ttttgcatcc
     1381 atcgggaata ctcattttta attggtaaca gcagtttacc tgcttttatg tcttcaacct
     1441 accatatagg tggcggtggc actggcaata taaagctgtt cttcattgtg taattcaacg
     1501 cgggcgacgg cgactttatt gcctgcacgc aacaggctac tggtagcagt aaaacgctcg
     1561 cccctgcctg ggcgcagata atcaacgcga agatcaatgg tccccatccg cgatagccgc
     1621 tggcgtagtt catcttcact gatggtttcg tggcgggtta aggtacttcc cacgcacacc
     1681 agaccggcgg cgacatccag cgccgacgca atgaccccgc cgtgcaaaat gctttgcgcc
     1741 cagttgccca ccatcattgg ctgattttta aaggccagct gtgcgaactc tttttcgtaa
     1801 cgctccagtt ccatccccaa tgcgcggtta aatggcatgt gataaacaaa catctcaccc
     1861 actaatttca gggcttgttc agcggtcagt acggcagaca tatcatcctt acacttcatt
     1921 ggttaatgaa atgttgattt tatgcttctt tgttgttggt ttctacttta ggaagggata
     1981 actaacggct atggagttaa gtatgtaaaa tagcccgcag aaaaatattc accttatcaa
     2041 taattcgtta cggagaacac gaccgatgcg gactctgcag ggctggttgt tgccggtgtt
     2101 tatgttgcct atggcagtat atgcacaaga ggcaacggtg aaagaggtgc atgacgcgcc
     2161 agcggtgcgt ggcagtatta tcgccaatat gctgcaggag catgacaatc cgttcacgct
     2221 ctatccttat gacaccaact acctcattta cacccaaacc agcgatctga ataaagaagc
     2281 gattgccagt tacgactggg cggaaaatgc gcgtaaggat gaagtaaagt ttcagttgag
     2341 cctggcattt ccgctgtggc gtgggatttt aggcccgaac tcggtgttgg gtgcgtctta
     2401 tacgcaaaaa tcctggtggc aactgtccaa tagcgaagag tcttcaccgt ttcgtgaaac
     2461 caactacgaa ccgcaattgt tcctcggttt tgccaccgat tactgttttg caggttggac
     2521 gctgcgcgat gtggagatgg ggtataacca cgactctaac gggcgttccg acccgacctc
     2581 ccgcagctgg aaccgccttt atactcgcct gatggcagaa aacggtaact ggctggtaga
     2641 agtgaagccg tggtatgtgg tgggtaatac tgacgataac ccggatatca ccaaatatat
     2701 gggttactac cagcttaaaa tcggctatca cctcggtgat gcggtgttga gtgcgaaagg
     2761 acagtacaac tggaataccg gttacggcgg cgcggagtta ggcttaagtt acccgatcac
     2821 caaacatgtg cgcctttata ctcaggttta cagcggctat ggcgaatcgc tcatcgacta
     2881 taacttcaac cagacccgtg tcggtgtagg agttatgcta aacgacttgt tttgatgaac
     2941 ggttgagtgg ttggcaaatc tggaacccag catccaggat taccctctca gagactaaaa
     3001 gcattgcagt ttctcgcgca ggcgctgaaa atagcgcctg tttttatttc aggcaatcgg
     3061 ggggaatgtg gcgcaggcgg aagtgttgaa tctggagtcc ggagctaaac aggttttaca
     3121 agaaaccttt ggctaccaac agtttcgccc cggccaggaa gaaattatcg acactgtgct
     3181 ttccggtcgc gattgcctgg tcgtcatgcc caccggtggc ggaaaatccc tttgctatca
     3241 aattcctgcc ttattgctaa acggccttac cgtggttgtt tcaccgctga tttcgttgat
     3301 gaaagatcag gtggatcaac tgcaagccaa cggcgtggcg gcggcgtgcc ttaactcgac
     3361 gcaaacccgc gagcagcaac ttgaagtgat gacaggctgc cgcaccgggc aaattcgtct
     3421 gctttatatc gccccggaac gcctgatgct ggataacttt cttgagcatc tggcgcactg
     3481 gaatccggtg ttattagccg ttgatgaagc gcactgtatc tcccaatggg gccacgattt
     3541 ccgcccggaa tatgccgcgc ttggtcagtt acgccagcgt ttcccgaccc tgccgtttat
     3601 ggcgctgact gccacagccg acgacaccac gcgccaggat atcgtgcgct tgctggggct
     3661 gaacgatccg ctgattcaaa tcagcagttt tgaccgtccg aatattcgct acatgctgat
     3721 ggagaaattc aaaccgctcg atcagttgat gcgctacgtg caggaacagc gcggtaagtc
     3781 cggcattatc tactgcaaca gccgggcgaa agtagaagac accgctgcgc gcctgcaaag
     3841 caagggtatt agcgcggcgg cctatcatgc cgggctggaa aataatgtcc gcgccgacgt
     3901 gcaggagaaa ttccagcgcg atgacctgca aattgtggtg gcgacggtgg cgttcggcat
     3961 gggcatcaat aaaccaaacg ttcgcttcgt ggtccacttt gatattccgc gcaatatcga
     4021 atcctattat caggaaaccg gtcgcgccgg gcgtgatggc ctgcctgcgg aagcgatgct
     4081 gttttacgat ccggctgata tggcgtggct gcgccgttgt ctggaagaga agccgcaggg
     4141 gcagttgcag gatattgagc gccacaaact caatgcgatg ggcgcgtttg ccgaagcgca
     4201 aacttgccgt cgtctggtat tgctgaacta ttttggcgaa gggcgtcagg agccgtgcgg
     4261 gaactgcgat atctgcctcg atccgccgaa acagtacgac ggttcaaccg atgctcagat
     4321 tgccctttcc accattggtc gtgtgaatca gcggtttggg atgggttatg tggtggaagt
     4381 gattcgtggt gctaataacc agcgtatccg cgactatggt catgacaaac tgaaagtcta
     4441 tggcatgggc cgtgataaaa gccatgaaca ttgggtgagc gtgatccgcc agctgattca
     4501 cctcggcctg gtgacgcaaa atattgccca gcattctgcc ctacaactga cagaggccgc
     4561 gcgcccggtg ctgcgcggcg aatcctcttt gcaacttgcc gtgccgcgta tcgtggcgct
     4621 caaaccgaaa gcgatgcaga aatcgttcgg cggcaactat gatcgtaaac tgttcgccaa
     4681 attacgcaaa ctgcgtaaat cgattgccga tgaaagtaat gtcccgccgt acgtggtgtt
     4741 taacgacgca accttgattg agatggctga acagatgccg atcaccgcca gcgaaatgct
     4801 cagcgttaac ggcgttggga tgcgcaagct ggaacgcttt ggtaaaccgt ttatggcgct
     4861 gattcgtgcg catgtcgatg gcgacgacga agagtagtca gcagcataaa aaagtgccag
     4921 gatgaagact ccgtaaacgt ttcccccgcg agtcaaatgt atgttgatgt tatttctcac
     4981 cgtcgccatg gtgcacattg tggcgcttat gagccccggt cccgatttct tttttgtctc
     5041 tcagaccgct gtcagtcgtt cccgtaaaga agcgatgatg ggcgtgctgg gcattacctg
     5101 cggtgtaatg gtttgggctg ggattgcgtt gcttggcctg catttgatta tcgaaaaaat
     5161 ggcctggctg catacgctta tggttggcgg tggcctgtat ctctgctgga tgggttacca
     5221 gatgctgcgc ggcgcgctga aaaaagaggc ggtttctgca cctgcgccac aggtcgagct
     5281 ggcgaaaagt ggacgcagtt tcctgaaagg tttactgacc aatctcgcta atccgaaagc
     5341 gattatctac tttggctcgg tgttctcatt gtttgtcggt gataacgttg gcactaccgc
     5401 gcgctggggc atttttgcgc tgatcattgt cgaaacgctg gcgtggttta tcgtcgttgc
     5461 cagcctgttt gccctgccgc aaatgcgccg tggttatcaa cgtctggcga agtggattga
     5521 tggttttgcc ggggcgttat ttgccggatt tggcattcat ttgattattt cgcggtgatg
     5581 ccagacgcgt cttcagagta agtcggataa ggcgtttacg ccgcatccga cattattttt
     5641 cacgcatgcc tcgccgatgc taacagcgct cccaccagca taaacaacga gccgaaaatc
     5701 ttattcagcg ccttcatctg ctttggtcct ttaatccata gagcaatccg ttgagcaagg
     5761 gtggcgtaac cgatcatcac aataatatcg accacaatag tggtgacgcc gagcacgata
     5821 tactgcatca gttgcggctg ttgcggcatg atgaattgcg gaaatagcgc cgccagaaac
     5881 acaatacttt tgggattggt gagattcaca aaaactgcgc gctggaacaa atgtcgacgc
     5941 gattgagtag aggccagcga tttaaggtca attgcaccag cggcgcgcca ctgctggatt
     6001 cccagccaaa tcaagtaagc cgcgcctgcc cacttcaaca cttcaaacgc aatcactgag
     6061 cgggaaaata gcgtccccaa ccccacgcca accagcacaa tatgaatcgc cagtccggtc
     6121 tgaagcccag caatagacgc caccgcgccg cgataaccgt ggttgagcga ggtggtcata
     6181 gtgttgattg caccagagcc tggcgacagg cttaaaatga tcgatgtcag caggtaggca
     6241 aaccaccatt ctaaggtcat gatgaactcc cggtgtgtct atttttgtgc cacaatacgc
     6301 tactgtcgca gcgttgtgtc aggcacgcta aaaaaaacga ttttacgtgg tttaagaggc
     6361 agattacccg atgtttcagc agcaaaaaga ctgggaaaca agagaaaacg cgtttgctgc
     6421 ttttaccatg ggaccgctga ctgatttctg gcgtcagcgt gatgaagcag agtttactgg
     6481 tgtggatgac attccggtgc gctttgtccg ttttcgcgca cagcaccatg accgggtggt
     6541 agtcatctgc ccggggcgta ttgagagcta cgtaaaatat gcggaactgg cctatgacct
     6601 gttccatttg gggtttgatg tcttaatcat cgaccatcgc gggcagggac gttccggtcg
     6661 cctgttagcc gatccgcatc tcgggcatgt taatcgcttt aatgattatg ttgatgatct
     6721 ggcggcattc tggcagcagg aggttcagcc cggtccgtgg cgtaaacgct atatactggc
     6781 acattcgatg ggcggtgcga tctccacatt atttctgcaa cgccatccag gtgtatgtga
     6841 cgccattgcg ctaactgcgc caatgtttgg gatcgtgatt cgtatgccgt catttatggc
     6901 acggcagatc ctcaactggg ccgaagcgca tccacgtttc cgtgatggct atgcaatagg
     6961 caccgggcgc tggcgcgcgt tgccgtttgc tatcaacgta ctgacccaca gcagacagcg
     7021 atatcgacgt aacttacgct tctatgctga tgacccaacg attcgcgtcg gtgggccgac
     7081 ctaccattgg gtacgcgaaa gtattctggc tggcgaacag gtgttagccg gtgcgggtga
     7141 tgacgccacg ccaacgcttc tcttgcaggc tgaagaggaa cgcgtggtgg ataaccgcat
     7201 gcatgaccgt ttttgtgaac tccgcaccgc cgcgggccat cctgtcgaag gaggacgacc
     7261 gttggtaatt aaaggtgctt accatgagat cctttttgaa aaggacgcaa tgcgctcagt
     7321 cgcgctccac gccattgttg attttttcaa caggcataac tcacccagcg gaaaccgctc
     7381 tacagaggtt taaatttctt atgtaccagg ttgttgcgtc tgatttagat ggcacgttac
     7441 tttctcccga ccatacgtta tccccttacg ccaaagaaac cctgaagctg ctcaccgcgc
     7501 gcggcatcaa ctttgtgttt gcgaccggtc gtcaccacgt tgatgtgggg caaattcgcg
     7561 ataatctgga gatcaagtcg tacatgatca cctccaacgg cgcgcgcgtt cacgatctgg
     7621 atggcaatct gatttttgct cataacctgg atcgcgacat tgccagcgat ctgtttggcg
     7681 tagtcaacga caatctggac atcattacta acgtttatcg cgacgacgaa tggtttatga
     7741 atcgccatcg cccggaagag atgcgctttt ttaaagaagc ggtgttcaaa tatgcgctgt
     7801 atgagcctgg attactggag ccggaaggcg tcagcaaagt gttcttcacc tgcgattccc
     7861 atgaacaact gctgccgctg gagcaggcga ttaacgctcg ttggggcgat cgcgtcaacg
     7921 ttagtttctc taccttaacc tgtctggaag tgatggcggg cggcgtttca aaaggccatg
     7981 cgctggaagc ggtggcgaag aaactgggct acagcctgaa ggattgtatt gcgtttggtg
     8041 acgggatgaa cgatgccgaa atgctgtcga tggcggggaa aggctgcatt atgggcagtg
     8101 cgcaccagcg tctgaaagac cttcatcccg agctggaagt gattggtact aatgccgacg
     8161 acgcggtgcc gcattatctg cgtaaactct atttatcgta atcgttcttt atttggtcag
     8221 ttgtcaacct gatacttcgc tacaatggat actcgttaat caaagagttt tccattgtgg
     8281 cgctacttat catcaccacg attctgtggg ccttctcctt tagcttttat ggcgagtacc
     8341 ttgcggggca cgtcgatagc tattttgcgg tgctggtgcg cgttggcctg gcggcactcg
     8401 tttttctgcc gtttctgcgt acccgtggca atagcctgaa aacggtcggc ctgtatatgc
     8461 tggtgggcgc gatgcagctc ggcgtgatgt atatgctgag tttccgcgct tatctctacc
     8521 tgacggtctc cgaattgttg ctgttcaccg tactgacgcc gctctacatc acgctgattt
     8581 atgacatcat gagtaagcgc cgtctgcgct ggggctatgc ttttagcgcc ttgctggcgg
     8641 tgattggtgc cgggattatt cgctatgatc aggttaccga ccatttctgg actggcttgt
     8701 tgctggtgca actctccaat atcacttttg ccatcggcat ggtgggttac aaacgcctga
     8761 tggaaactcg cccgatgccg cagcataacg cctttgcgtg gttctatctt ggcgcgttcc
     8821 tggtggcggt aattgcatgg ttcttgctgg gaaatgcgca gaaaatgccg caaaccacgt
     8881 tgcaatgggg cattctggtg tttcttggcg tggtggcttc cgggattggc tactttatgt
     8941 ggaactacgg cgcgacgcag gtggacgccg gaacgctggg cattatgaat aatatgcacg
     9001 ttccggcagg gctgctggta aacctggcta tctggcacca acagccgcac tggccaacgt
     9061 tcattacagg cgcgctggtg atcctggcct cactgtgggt gcatcgtaag tgggtcgctc
     9121 cgcgctcttc acaaacggca gatgatcgca ggcgtgattg cgcgctgagc gaataaatgc
     9181 ttctgtaatc ggctgacgct gctcgccatc gcgcacggcg gcgtacagtc ggctccacaa
     9241 gccttcgccc agggttttgg tcaccaccag accttggcgc tcaaaactct ctactaccca
     9301 atgcggtagc gcggcaatac ccatccgcgc ggcaaccatc tgaatcaaca ataaggtgtt
     9361 atcgacgctt ttcagtgacg ggctgacgcc tgccggctga agaaaatgcc gccagacatc
     9421 cagtcgacta cgctgcaccg gataaatcaa cagcgtctcg ctggcgagat cttccggtgt
     9481 aattcgcgtt ttcgccgcca gtggatggtc aggtgctaac accagacgca cttcatagtc
     9541 gaacatcggc gaataatgca ggccactgcg cggcaggata tcggaggtca tcaccagatc
     9601 cagctcgccc tgttgcaacg caggctgcgg gtcaaacgtc acgcccgatt taaaatccat
     9661 ctctacctgc ggccagttct tatggaaatt ttctaacgcg ggcgtcagcc actgaataca
     9721 gctatggcac tcaatggcaa tgcgcagacg cgtctgctgc ggttcattgc aggcttgcag
     9781 tgcctggctg atttgcggca gcacctggtt tgccagttgc aacaggattt ctccctgcgg
     9841 tgtaaagcgt agcggctggc tcttacgcac aaatagccgg aagccaaggc gttgttccag
     9901 atcgctaaac tggtgagaca gggcggattg cgtctgatgc aacgtcgccg cagcggctgc
     9961 gagagagccg cagttccgca acgcttgtag cgttttcagg tgttttactt cgatcatgaa
    10021 agtccttcac ttcggcatga ataatttgcg cttgaggaat atacagtaac cgccaattat
    10081 ggatgtgtaa acatctggac ggctaaaatc cttcgtcttt taaatttatg gtgcgttggc
    10141 tgcgtttctc caccccggtc acttacttca gtaagctccc ggggatgaat aaacttgccg
    10201 ccttccctaa attcaaaatc cataggattt acatataatt agaggaagaa aaatgacaat
    10261 attgaatcac accctcggtt tccctcgcgt tggcctgcgt cgcgagctga aaaaagcgca
    10321 agaaagttat tgggcgggga actccacgcg tgaagaactg ctgacagtag ggcgtgaact
    10381 gcgtgctcgt cactgggatc aacaaaagca agcgggtatc gacctgctgc cggtgggcga
    10441 ttttgcctgg tacgatcatg tactgaccac cagtttgctg ctgggtaatg ttccgccacg
    10501 tcatcagaac aaagacggtt cggtagatat cgacaccctg ttccgtattg gtcgtggacg
    10561 tgcgccgact ggcgaacctg cggcggcagc ggaaatgacc aaatggttta acaccaacta
    10621 tcactacatg gtgccggagt tcgttaaagg ccaacagttc aaactgacct ggacgcagct
    10681 gctggaggaa gtggacgagg cgctggcgct gggccacaac gttaagcctg tgctgctggg
    10741 gccggttact tacctgtggc tggggaaagt gaaaggtgaa cagtttgacc gccttagcct
    10801 gctgaacgac attctgccgg tttatcagca agtgctggca gaactggcga aacgcggcat
    10861 cgagtgggta cagattgatg aacccgcgct ggtactggaa ctaccacagg cgtggctgga
    10921 cgcatacaaa cccgcttacg acgcgctcca gggacaggtg aaactgctgc tgaccaccta
    10981 ttttgaaggc gtaacgccaa atctcgacac gattactgcg ctgcctgttc agggtctgca
    11041 tgttgatctc gtacatggta aagatgacgt tgctgaactg cacaagcgcc tgccttctga
    11101 ctggttgctg tctgcgggtc tgatcaatgg tcgtaacgtc tggcgcgccg atcttaccga
    11161 gaaatatgcg caaattaagg acattgtcgg caaacgtgat ttgtgggtgg catcttcctg
    11221 ctcgttgctg cacagcccca tcgacctgag cgtggaaacg cgtcttgatg cagaagtgaa
    11281 aagctggttt gccttcgccc tacaaaaatg ccatgaacta gcactgctgc gcgatgcgct
    11341 gaacagtggt gacacggcag ctctggcaga gtggagcgcc ccaattcagg cgcgtcgtca
    11401 ctctactcgt gtacataatc cggcggtaga aaagcgtctg gcggcgatca ccgcccagga
    11461 cagccagcgt gcgaatgtct atgaagtgcg tgctgaagcc cagcgtgcac gttttaaact
    11521 gccagcgtgg ccgaccacca cgattggttc cttcccgcaa accacggaaa ttcgtaccct
    11581 gcgtctggat ttcaaaaagg gcaatctcga cgccaataac taccgcacgg gcattgcgga
    11641 acatatcagg caggccattg ttgaacagga acgtttggga ctggatgtgc tggtacatgg
    11701 cgaggccgag cgtaatgaca tggtggaata ctttggcgag cacctcgacg ggtttgtctt
    11761 tacgcaaaac ggttgggtac agagctacgg ttcccgctgc gtgaagccac cgattgttat
    11821 tggtgacgtt agtcgcccgg caccgattac cgtggagtgg gcgaagtatg cgcaatctct
    11881 gactgataaa ccggtgaaag ggatgctgac tggcccggtg actattctct gctggtcgtt
    11941 cccgcgtgaa gatgtcagcc gtgaaaccat cgccaaacag attgcgctgg cgctgcgtga
    12001 tgaagtggcc gatctggaag ccgctggaat tggcatcatc cagattgacg aaccggctct
    12061 gcgcgaaggt ttaccgctgc gtcgtagcga ctgggatgcg tatctccagt ggggcgtaga
    12121 ggccttccgt atcaacgccg ccgtggcgaa agatgacaca caaatccaca ctcacatgtg
    12181 ttattgcgag ttcaacgaca tcatggattc gattgcggca ctggacgcag acgtcatcac
    12241 catcgaaacc tcgcgttctg acatggagtt gctggagtcg ttcgaagagt ttgattatcc
    12301 gaatgaaatc ggtccaggcg tctatgacat tcactcgcca aacgtaccga gcgtggaatg
    12361 gattgaagcc ttgctgaaga aagcggcaaa acgcattccg gcagagcgcc tgtgggttaa
    12421 cccggactgt ggcctgaaaa cgcgcggctg gccagaaacc cgcgcggcac tggcgaacat
    12481 ggtgcaggcg gcgcagaatt tgcgtcgggg atgatgaata ttaacgagtc actgtaaatt
    12541 gcagtacgat gaaagaggca aaactgcctg atactaggcc tacataattt atgcaattta
    12601 ttgaattttt gggttttgtt ggccggacaa ggcgttcacg ccgcatccgg catgaacaac
    12661 gcgcactttg ccaataatct gatggcttcc atcaccggaa gccattttgc tatctgcgcc
    12721 cgttcagata cagaaataac cgaatcattt ttacgcaaaa tataatcctg agcgatttgt
    12781 tcagtaatca acgctacgtt atttattctc acttttcacg aaattgtatc ccttttcagt
    12841 aacgtggtag tgaggcatag acttatgtcc gctatcctca ataataacca aaccctcgtt
    12901 agctaatttg ttaacaatgt taacaacatt accgaatcct ggaatttgcc ttatggatag
    12961 tgtgcgatcg agattcatcc agttccagtt tatatctttc tcagccataa ttttcagtac
    13021 gttaatttca gtttcagtta tcatagtcaa gtgcatcctg gtggtataag tcattaagtt
    13081 ctcccttaag tttataatct tctagagtag cagtatgcgt a
//
LOCUS       DADMKF010000113        12996 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.115, whole genome shotgun sequence.
ACCESSION   DADMKF010000113 DADMKF010000000
VERSION     DADMKF010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12996
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.115"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            96..1391
                     /locus_tag="JNC24_002954"
     CDS             96..1391
                     /locus_tag="JNC24_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HAY9159650.1"
                     /translation="MDADSSDSTVTPGRLPDGLSSCPCFYLGKKLMNLSANAQATLLL
                     TSDFSRAAASEYKPLSNSEWGKFALWLKHQRISPAELLVPQPQEKLTGWSDPRISQER
                     ILGLLARGHSLALAVDKWQRAGLWILTRGDADYPVRLKNRLRTDAPPVLFGCGNKALL
                     QAEGMAIVGSRDAPTDDLRYTQQLAAKLAQQGICVISGGARGIDECAMASALEAGGTA
                     VGVLADSLLKTSTLVKWREGLIAGNLVLISPFYPEVRFTVGNAMARNKYIYCLAESAM
                     VVRAGMTGGTITGAMEALKHQWLPVQVKPNQDMQSANSRLVENGASWSAEQAENVTIR
                     LPDVPGLMYDRALRNAQPELFSLHEDDANYAVMPAYTPVDFYQLFVAELAILAKESIS
                     IERLASCTGLTIEQISVWLNRAEEEGRVIRLGEGHYQFR"
     gene            complement(1437..2030)
                     /gene="yhgN"
                     /locus_tag="JNC24_002955"
     CDS             complement(1437..2030)
                     /gene="yhgN"
                     /locus_tag="JNC24_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HAY9159651.1"
                     /translation="MNAIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            2222..3325
                     /gene="asd"
                     /locus_tag="JNC24_002956"
     CDS             2222..3325
                     /gene="asd"
                     /locus_tag="JNC24_002956"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HAY9159652.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            3598..5784
                     /gene="glgB"
                     /locus_tag="JNC24_002957"
     CDS             3598..5784
                     /gene="glgB"
                     /locus_tag="JNC24_002957"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HAY9159653.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPVYRQYHHDKLT
                     FGILYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SMHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            5781..7754
                     /gene="glgX"
                     /locus_tag="JNC24_002958"
     CDS             5781..7754
                     /gene="glgX"
                     /locus_tag="JNC24_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HAY9159654.1"
                     /translation="MTRLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGHSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIEG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALHEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYACACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIALGGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGSLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLTWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITAVWQGPAHGLCVFQR"
     gene            7772..9067
                     /gene="glgC"
                     /locus_tag="JNC24_002959"
     CDS             7772..9067
                     /gene="glgC"
                     /locus_tag="JNC24_002959"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HAY9159655.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            9067..10500
                     /gene="glgA"
                     /locus_tag="JNC24_002960"
     CDS             9067..10500
                     /gene="glgA"
                     /locus_tag="JNC24_002960"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HAY9159656.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KRQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDNNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            10519..12966
                     /gene="glgP"
                     /locus_tag="JNC24_002961"
     CDS             10519..12966
                     /gene="glgP"
                     /locus_tag="JNC24_002961"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HAY9159657.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEMR
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
BASE COUNT         3040 a         3228 c         3576 g         3152 t
ORIGIN      
        1 ttccaccagt gtcaaaatct cgacggggcg tttgttatta cccctccttt gatgcctggt
       61 ccggcgttgc tggttgacga tatcgtggat tctgcatgga cgctgacagt tctgacagca
      121 ctgttacgcc aggcaggttg cccgacggtt tatcctcttg cccttgcttc tacctcggta
      181 aaaaattgat gaatctttca gccaatgcac aagcaactct cctgctaacc agcgattttt
      241 ctcgcgcggc ggcgagtgag tataaacctc ttagtaatag tgaatggggg aagtttgcat
      301 tatggctgaa gcaccaacgt atcagtcccg ccgagcttct ggtgccgcaa ccgcaagaga
      361 aacttacagg ctggagcgat ccgcgtattt ctcaggagcg tattcttggc ttgctggcgc
      421 gtggtcatag tctggcgttg gcggtagata agtggcaacg cgccggttta tggatcttaa
      481 cccgcggaga tgctgattat cccgttcgct tgaaaaaccg attgcgaacg gatgcacctc
      541 ccgttttatt tggctgcggg aataaagcat tactgcaagc ggaaggtatg gcgattgttg
      601 gctcgcgaga tgctccgact gacgatttgc gctataccca acaactggcc gcgaaactgg
      661 cccaacaggg gatttgcgtt atctctggtg gtgcgcgagg tattgatgaa tgtgcaatgg
      721 cgtcggcact ggaggccggg ggaactgccg ttggcgtatt agctgatagc ttgttaaaaa
      781 cgagtacgtt agtgaaatgg cgtgaagggc ttatagcagg caacctggtg ttgatttcgc
      841 cgttttaccc agaggtacgt ttcaccgtcg gcaatgcgat ggcgcgaaat aaatatattt
      901 attgccttgc tgaaagcgca atggttgtac gtgcgggaat gaccggtgga acgataaccg
      961 gggcgatgga ggcattaaaa catcagtggc tgcctgtgca ggttaaacca aatcaggata
     1021 tgcaatcagc caattcacga ttagtagaaa atggggcgtc atggagtgct gaacaggctg
     1081 agaatgtgac gatcagactg ccagacgttc ctgggctgat gtatgacaga gcactccgta
     1141 acgctcaacc agaactgttt tcgctgcatg aagatgacgc aaattacgca gtaatgcccg
     1201 cgtatacgcc tgtcgatttt tatcaactct ttgtggcgga actggcgatc cttgcaaagg
     1261 aatcgataag tattgaaagg ctggcgtctt gtactggttt aaccatcgaa caaattagtg
     1321 tgtggctgaa ccgcgcagaa gaagagggaa gggttatccg attgggcgaa ggtcattatc
     1381 agttcaggta ataaaacagg tgccgcgtct ggttgtgtgc agcacctgtt tgaaccttac
     1441 cccttcatcc acattcgaat gccgtcgagg aacatctggg ttgccatcat caccagaatc
     1501 aatcccatca ggcgttcaag tgcgttcacc cctttctcgc ccagcagacg taaaaatagc
     1561 gaagactgta gcaggatgac aaaggtgccg ccccaggcca gcagcagagc aatcaccaga
     1621 tgtcccatct gattcgggta ctgatgagac aacaacatca gcgtggcgag aatagtcggc
     1681 ccggcgacta acggaattgc caacggcacg ataaatggct cttcacctgc cggaagcccg
     1741 ctgctatttc ctgaagcgct ggggaaaatc atcttaatgg cgattagaaa cagaatgatg
     1801 ccgccagaaa tggagacggt ttctgctcgc aggctgagaa atgccagaat tttctcaccc
     1861 gcaaacagga acaccagcat caccaggaga gcaataagca actctcgcac catgattgcc
     1921 cgccgtcttt tcggttcagt atgtttcagt acggacatga aaataggcag gtttccgagc
     1981 ggatccataa tcaggatcaa taaaactgct gcagaaatga ttgcattcat aactcaaatt
     2041 ccctgataat tgccgcggac tttctgcgtg ctaacaaagc aggataagtc gcattactga
     2101 tggcttcgct atcattgatt aatttcactt gcgactttgg ctgctttttg tatggtgaaa
     2161 gatgtgccaa gaggagaccg gcacatttat acagcacaca tctttgcagg aaaaaacgct
     2221 tatgaaaaat gttggtttta tcggctggcg cggtatggtc ggctccgttc tcatgcaacg
     2281 catggttgaa gagcgcgact tcgacgccat tcgccctgtc ttcttttcta cttctcagct
     2341 tggccaggct gcgccgtctt ttggcggaac cactggcaca cttcaggatg cctttgatct
     2401 ggaggcgcta aaggccctcg atatcattgt gacctgtcag ggcggcgatt ataccaacga
     2461 aatctatcca aagcttcgtg aaagcggatg gcaaggttac tggattgacg cagcatcgtc
     2521 tctgcgcatg aaagatgacg ccatcatcat tcttgacccc gtcaatcagg acgtcattac
     2581 cgacggatta aataatggca tcaggacttt tgttggcggt aactgtaccg taagcctgat
     2641 gttgatgtcg ttgggtggtt tattcgccaa tgatcttgtt gattgggtgt ccgttgcaac
     2701 ctaccaggcc gcttccggcg gtggtgcgcg acatatgcgt gagttattaa cccagatggg
     2761 ccatctgtat ggccatgtgg cagatgaact cgcgaccccg tcctctgcta ttctcgatat
     2821 cgaacgcaaa gtcacaacct taacccgtag cggtgagctg ccggtggata actttggcgt
     2881 gccgctggcg ggtagcctga ttccgtggat cgacaaacag ctcgataacg gtcagagccg
     2941 cgaagagtgg aaagggcagg cggaaaccaa caagatcctc aacacatctt ccgtaattcc
     3001 ggtagatggt ttatgtgtgc gtgtcggggc attgcgctgc cacagccagg cattcactat
     3061 taaattgaaa aaagatgtgt ctattccgac cgtcgaagaa ctgctggctg cgcacaatcc
     3121 gtgggcgaaa gtcgttccga acgatcggga aatcactatg cgtgagctaa ccccagctgc
     3181 cgttaccggc acgctgacca cgccggtagg ccgcctgcgt aagctgaata tgggaccaga
     3241 gttcctgtca gcctttaccg tgggcgacca gctgctgtgg ggggccgcgg agccgctgcg
     3301 tcggatgctt cgtcaactgg cgtaatcttt attcattaaa tctggggcgc gatgccgccc
     3361 ctgttagtgc gtaatacagg agtaagcgca gatgtttcat gatttaccgg gagttaaata
     3421 gagcattggc tattctttaa gggtggctga atacatgagt attcacagcc ttacctgaag
     3481 tgaggacgac gcagagagga tgcacagagt gctgcgccgt tcaggtcaaa aaaatgtcac
     3541 aaccagaagt caaaaatcca attggatggg gtgacacaat aaaacaggaa gacaagcatg
     3601 tccgatcgta tcgatagaga cgtgattaac gcgctaattg caggccattt tgcggatcct
     3661 ttttccgtac tgggaatgca taaaaccacc gcgggactgg aagtccgtgc ccttttaccc
     3721 gacgctaccg atgtgtgggt gattgaaccg aaaaccgggc gcaaactcgc aaaactggag
     3781 tgtctcgact cacggggatt ctttagcggc gtcattccgc gacgtaagaa ttttttccgc
     3841 tatcagttgg ctgttgtctg gcatggtcag caaaacctga ttgatgatcc ttaccgtttt
     3901 ggtccgctaa tccaggaaat ggatgcctgg ctattatctg aaggtactca cctgcgcccg
     3961 tatgaaacct taggcgcgca tgcagatact atggatggcg tcacaggtac gcgtttttct
     4021 gtctgggctc caaacgcccg tcgggtctcg gtggttgggc aattcaacta ctgggacggt
     4081 cgccgtcacc cgatgcgcct gcgtaaagag agcggcattt gggaactgtt tatccctggg
     4141 gcgcataacg gtcagctcta taaatacgag atgattgatg ccaatggcaa cttgcgtctg
     4201 aagtccgacc cttatgcctt cgaagcgcaa atgcgcccgg aaaccgcgtc tcttatttgc
     4261 gggctgccgg aaaaggttgt acagactgaa gagcgcaaaa aagcgaatca gtttgatgcg
     4321 ccaatctcta tttatgaagt tcacctgggc tcctggcgtc gccacaccga taacaatttc
     4381 tggttgagct accgcgagct ggccgatcaa ctggtgcctt atgctaaatg gatgggcttt
     4441 acccacctcg aactactgcc cattaacgag catcccttcg atggcagttg gggttatcag
     4501 ccaaccggcc tgtatgcacc aacccgccgt tttggtactc gcgacgactt ccgttatttc
     4561 attgatgccg cacacgcagc gggtctgaac gtgattctcg actgggtgcc aggccacttc
     4621 ccgaccgatg actttgcgct tgccgaattt gatggcacga acttgtatga acacagcgat
     4681 ccgcgcgaag gctatcatca ggactggaat acgctgatct acaactatgg tcgccgtgaa
     4741 gtcagtaact tcctcgtcgg taacgcgctt tactggattg aacgttttgg tattgatgcg
     4801 ctgcgcgtcg atgcggtggc gtcaatgatt tatcgcgact acagccgtaa agagggggag
     4861 tggatcccga acgaatttgg cgggcgcgag aatcttgaag cgattgaatt cttgcgtaat
     4921 accaaccgta ttcttggtga gcaggtttcc ggtgcggtga caatggctga ggagtctacc
     4981 gatttccctg gcgtttctcg tccgcaggat atgggcggtc tgggcttctg gtacaagtgg
     5041 aacctcggct ggatgcatga caccctggac tacatgaagc tcgacccggt ttatcgtcag
     5101 tatcatcacg ataaactcac cttcgggatt ctctacaact acactgaaaa cttcgtcctg
     5161 ccgttgtcgc atgatgaagt ggtccacggt aaaaaatcga ttctcgaccg catgccgggc
     5221 gacgcatggc agaaattcgc taacctgcgc gcctactatg gctggatgtg ggcattcccg
     5281 ggcaagaaac tgctgttcat ggggaacgaa tttgcccaag gccgtgagtg gaaccatgac
     5341 gccagcctcg actggcatct gttggaaggc ggcgataact ggcaccacgg tgtccagcgt
     5401 ctggtgcgcg atctgaacct cacctaccgc caccataaag caatgcatga actggatttt
     5461 gacccgtacg gctttgaatg gctagtggtg gatgacaaag aacgctcggt gctgatcttt
     5521 gtgcgtcgcg ataaagaggg taacgaaatt atcgttgcca gtaactttac gccggtgccg
     5581 cgtcatgatt atcgcttcgg cattaatcag ccaggcaaat ggcgtgaaat cctcaatacc
     5641 gattccatgc actatcacgg cagtaatgca ggcaatggcg gcacggtaca cagcgatgag
     5701 attgccagcc acggtcgtca gcattcacta agcctgacac taccaccgct ggccactatc
     5761 tggctggttc gggaggcaga atgacacgac tcgccattgg caaacccgct cccctcggcg
     5821 cgcattacga cggtcagggc gtcaacttca cacttttctc cgctcatgcc gagcgggtag
     5881 agctgtgtgt ctttgacgcc aatggccagg aacatcgcta tgacttgcca gggcacagtg
     5941 gcgacatttg gcacggttat ctgccggatg cgcgcccggg tttgcgttat ggttatcgcg
     6001 ttcatggccc ctggcaaccc gccgaggggc atcgctttaa cccggcgaag ttgttgattg
     6061 atccttgcgc gcggcaaatt gaaggggaat tcaaagataa cccgctgctg cacgccggtc
     6121 ataatgaacc tgactatcgc gacaacgccg ccattgcgcc gaaatgcgta gtggtggttg
     6181 atcactatga ctgggaagat gatgccccgc cgcgcacgcc gtggggcagc accatcattt
     6241 atgaagccca tgttaaagga ttaacgtacc tgcacccgga gatcccggtc gagatccgtg
     6301 gcacttataa agccctcggg catccggtga tgatcaacta tttgaaacag ttgggcatta
     6361 ccgcgctgga actgctgccg gtggcgcagt ttgccagtga accacgtctg caacgcatgg
     6421 ggctaagtaa ctactggggt tacaacccgg tggcgatgtt tgcgctgcat ccggcgtatg
     6481 cctgctcgcc agaaacggcg ctgcatgagt ttcgcgatgc aatcaaagca ctgcataaag
     6541 cgggtatcga agtcattctt gatatcgtgc tcaaccatag tgcggaactg gacctcgacg
     6601 gcccgttatt ctcgctgcgt gggatcgaca accgtagcta ttattggata agagaagacg
     6661 gcgattatca caactggacc ggttgtggca acacgctcaa tttgagtcat ccggcggtgg
     6721 tggattatgc ctgcgcctgc ctgcgttatt gggtagaaac ctgtcacgtc gatggtttcc
     6781 gctttgatct ggcggcagtc atgggccgta cgccagagtt ccgtcaggat gcgccgttgt
     6841 ttaccgctat ccagaactgc ccggtgctct cgcaggtgaa gttaattgct gaaccgtggg
     6901 atatcgccct tggtggttat caggtgggaa atttcccgcc gctgtttgcc gagtggaacg
     6961 atcatttccg cgatgctgcc cgtcgtttct ggctacatta tgatttgcct ctgggggcgt
     7021 ttgccgggcg ttttgctgcc tccagcgatg tttttaaacg taatggtcgt ctgccgagtg
     7081 ccgcgattaa tctcgtcact gcgcatgacg gttttacgct tcgcgactgc gtttgcttca
     7141 accataaaca caatgaagca aacggagaag aaaatcgcga cgggaccaac aacaattaca
     7201 gtaacaatca tggtaaagaa gggttaggcg gttctcttga cctggttgaa cggcggcgcg
     7261 acagtattca cgccctgtta acaacgttgt tgctctccca gggtacgccg atgttactgg
     7321 ccggtgacga acacggtcac agccagcatg gcaataacaa tgcctactgt caggataacc
     7381 aattaacctg gttggactgg tcgcaggcaa gcagtggttt aaccgcattt accgccgcgt
     7441 taatccatct gcgcaagcgc attcccgctt tggtggagaa tcgctggtgg gaagaaggcg
     7501 acggcaatgt ccgttggcta aatcgatatg ctcaaccttt aagcacggat gagtggcaaa
     7561 acgggccgaa acagctgcaa attctgctct cggatcgctt tttgatcgca attaacgcca
     7621 cgcttgaggt aacagagatt gttttacctg ctggggagtg gcacgccatt cccccattcg
     7681 ctggagagga taacccagtg attacggctg tctggcaggg acctgcacac ggattgtgtg
     7741 tgttccagag atgataaaaa aggagttagt catggttagt ttagagaaga acgatcactt
     7801 aatgttggcg cgccagctgc cattgaaatc tgttgccctg atactggcgg gaggacgtgg
     7861 tacccgcctg aaggatttaa ccaataagcg agcaaaaccg gccgtacact tcggcggtaa
     7921 gttccgcatt attgactttg cgctgtctaa ctgcatcaac tctgggatcc gtcgtatggg
     7981 cgtgatcacc cagtaccagt cccacactct ggtgcagcac attcagcgcg gctggtcatt
     8041 cttcaatgaa gaaatgaacg agtttgtcga tctgctgcca gcacagcaga gaatgaaagg
     8101 ggaaaactgg tatcgcggca ccgcagatgc ggtcacccaa aacctcgaca ttatccgccg
     8161 ttataaagcg gaatacgtgg tgatcctggc gggcgaccat atctacaagc aagactactc
     8221 gcgtatgctt atcgatcacg tcgaaaaagg cgcacgttgc accgttgctt gtatgccagt
     8281 accgattgaa gaagcctccg catttggcgt tatggcggtt gatgagaacg ataaaattat
     8341 cgaattcgtt gaaaaacctg ctaacccacc gtcaatgccg aacgatccga gcaaatctct
     8401 ggcgagtatg ggtatctacg tctttgacgc cgactatctg tatgaactgc tggaagaaga
     8461 cgatcgtgat gagaactcca gccacgactt tggcaaagat ttgattccca agatcaccga
     8521 agccggtctg gcctatgcgc acccgttccc gctctcttgc gtacaatccg acccggatgc
     8581 cgagccgtac tggcgcgatg tgggtacgct ggaagcttac tggaaagcga acctcgatct
     8641 ggcctctgtg gtgccggaac tggatatgta cgatcgcaat tggccaattc gcacctacaa
     8701 tgaatcatta ccgccagcga aattcgtgca ggatcgctcc ggtagccacg ggatgaccct
     8761 taactcactg gtatccggcg gctgtgtgat ctccggttcg gtggtggtgc agtccgttct
     8821 gttctcgcgc gttcgcgtga attcattctg caacattgat tccgccgtat tgttaccgga
     8881 agtatgggta ggtcgctcgt gccgtctgcg ccgctgcgtc atcgatcgtg cttgtgttat
     8941 tccggaaggc atggtgattg gtgaaaacgc agaggaagat gcacgtcgtt tctatcgttc
     9001 agaagaaggc atcgtgctgg taacgcgcga aatgctacgg aagttagggc ataaacagga
     9061 gcgataatgc aggttttaca tgtatgttca gagatgttcc cgctgcttaa aaccggcggt
     9121 ctggctgatg ttattggggc attacccgca gcacaaatcg cagacggcgt tgacgctcgc
     9181 gtactgttgc ctgcatttcc cgatattcgc cgtggcgtga ccgatgcgca ggtagtatcc
     9241 cgtcgtgata ccttcgccgg acatatcacg ctgttgttcg gtcattacaa cggggttggc
     9301 atatacctga ttgacgcgcc gcatctctat gatcgtccgg gaagcccgta tcacgatacc
     9361 aacttatttg cctataccga caacgtattg cgttttgcgc ttctggggtg ggttggggca
     9421 gaaatggcca gcgggcttga cccattctgg cgtcctgatg tggtgcatgc gcacgactgg
     9481 catgcaggcc ttgcgcctgc gtatctggcg gcgcgcgggc gtcctgcgaa gtcggtgttt
     9541 actgtgcaca acctggccta tcaaggcatg ttttatgcac atcacatgaa tgacatccaa
     9601 ttgccatggt cattctttaa tattcatggg ctggaattca acggacaaat ctctttcctg
     9661 aaggccggtc tgtactatgc cgatcacatt acggcggtca gtccaaccta cgctcgcgag
     9721 atcaccgaac cgcagtttgc ctacggtatg gaaggtctgt tgcaacagcg tcatcgtgaa
     9781 gggcgtcttt ccggcgtact gaacggcgtg gacgagaaaa tctggagtcc agagacggac
     9841 ttactgttgg cctcgcgcta cacccgcgat acgttggaag ataaagcgga aaataagcgc
     9901 cagttacaaa tcgcaatggg gcttaaggtt gacgataaag tgccgctttt tgcagtggtg
     9961 agccgtctga ccagccagaa aggtctcgac ctggtgctgg aagccttacc gggtcttctg
    10021 gagcagggcg ggcagctggc gctactcggc gcgggcgatc cggtgctgca ggaaggtttc
    10081 cttgcggcgg cagcggaata ccccggtcag gtgggcgttc agattggcta tcacgaagca
    10141 ttttcgcatc gcattatggg cggcgcggac gtcattctgg tgcccagccg ttttgaaccg
    10201 tgcggcttaa cgcaacttta tggattgaag tacggtacgc tgccgttagt gcggcgcacc
    10261 ggtgggcttg ctgatacggt ttctgactgt tctcttgaga accttgcaga tggcgtcgcc
    10321 agtgggtttg tctttgaaga taataatgcc tggtcgctgt tacgggctat tcggcgtgct
    10381 tttgtactgt ggtcccgtcc ttcactgtgg cggtttgtgc aacgtcaggc tatggcaatg
    10441 gattttagct ggcaggtcgc ggcgaagtcg taccgtgagc tttactatcg cttgaaatag
    10501 ttttcaggaa acgcctatat gaatgctccg tttacatatt catcgcccac gcttagcgta
    10561 gaagctctta agcactctat cgcttacaag ctgatgttta cgattggaaa ggacccggtc
    10621 gtcgccaata aacatgaatg gctgaacgca acgttatttg ctgtgcgcga tcgtctcgtg
    10681 gagcgctggt tacgttcaaa ccgtgcccag ttgtcgcaag aaacacgtca ggtttactac
    10741 ctgtcgatgg agtttttgat tggccgtacg ctctccaacg ccatgttgtc gctaggaatt
    10801 tacgaagatg tacagggcgc actggaagcg atggggttaa atctcgaaga gctgattgat
    10861 gaagaaaatg acccaggcct cggtaacggt ggcctgggac gtctggcggc ttgcttcctt
    10921 gattctctgg cgacgttagg gttgccgggg cgcggttacg gcatccgcta tgactacggt
    10981 atgttcaagc agaacatcgt taacggtagc cagaaagagt cgccagacta ctggctggaa
    11041 tacggtaacc cgtgggaatt caaacgccac aacacgcgct ataaagtccg ttttggcggt
    11101 cgcattcagc aggaaggtaa aaaaacgcgc tggattgaaa ccgaagagat tctgggagtc
    11161 gcttacgatc agataatccc tggttacgac accgacgcga ccaacacgct gcgtttgtgg
    11221 agtgcgcaag ccagtagcga aattaacctc ggtaaattca accagggtga ctacttcgcg
    11281 gcagtggaag ataaaaacca ctccgagaac gtatctcgcg tactgtatcc ggatgactcc
    11341 acctactccg ggcgtgagct gcgcctgcgt caggaatact tcctggtttc ctcgaccatt
    11401 caggacattt taagccgcca ttatcagttg cataaaacct acgataacct ggcggataaa
    11461 atcgcgattc atctcaatga tacccatccg gtactgtcga ttcctgagat gatgcgtctg
    11521 ctgatcgatg agcaccaatt tagctgggac gacgcgtttg aggtgtgttg tcaggtcttc
    11581 tcctacacta accacacgct gatgagcgag gcgctggaaa cctggccggt tgatatgctg
    11641 ggtaaaattc tgccgcgtca cctgcagatc atctttgaaa tcaacgacta tttcctgaaa
    11701 accttgcagg aacagtatcc gaacgatacc gatctgctgg gacgggcgtc gatcattgat
    11761 gaatccaacg gtcgtcgtgt gcgtatggcc tggctggcgg ttgttgtgag ccacaaagtt
    11821 aacggtgtat cggaactgca ctctaatctg atggtgcaat cgttgtttgc cgactttgcg
    11881 aaaatcttcc cgggtcgttt caccaacgtc accaacggtg tgacgccgcg tcgctggctg
    11941 gcggtagcga acccatcgct ttcagccgtg ctggacgaac acctgggccg taactggcgc
    12001 accgacctta gcctgcttaa tgagctgcaa caacactgtg atttcccaat ggttaatcac
    12061 gctgtgcatc aggcgaagct ggagaacaaa aagcgtctgg cagagtatat cgcccagcag
    12121 ctgaatgtgg tggtgaatcc aaaggcgttg ttcgatgtac aaatcaaacg tattcacgaa
    12181 tacaaacgtc aattgatgaa tgtgttgcat gtgattaccc gctataaccg catcaaggcc
    12241 gacccggatg cgaagtgggt accgcgcgtg aatatttttg gcggtaaggc ggcttcggcc
    12301 tattacatgg cgaagcacat tattcatttg atcaatgacg tagcgaaagt gatcaacaac
    12361 gatccgcaga ttggcgataa gctgaaagtc gtgttcatcc cgaactacag cgttagcctg
    12421 gcgcagttga tcattccggc ggcagatctg tctgaacaga tttcgctggc agggacggaa
    12481 gcttccggca ccagtaacat gaagtttgcg cttaacggtg cgctgactat cggtacgttg
    12541 gacggtgcga atgtcgagat gcgggatcat gtcggtgctg acaatatctt tatttttggt
    12601 aacacagcgg aagaagtgga agaactgcgt cgtcagggct acaaaccgcg tgaatactac
    12661 gagaaagatg aggagctgca tcaggtgctg acgcaaatcg gcagcggtgt attcagtccg
    12721 gaagatccgg gtcgctatcg cgatctggtt gattcgctga tcaacttcgg cgatcactat
    12781 caggttctgg cggattatcg cagctatgtc gattgtcagg ataaagtcga cgaactctac
    12841 gagcttcagg aagagtggac cgcaaaagcg atgctgaaca ttgccaatat gggctacttc
    12901 tcttctgacc gtactatcaa agagtacgcc gatcatatct ggcatatcga tccggtgaga
    12961 ttgtaagttc accaataaat agaacggggc caaagg
//
LOCUS       DADMKF010000114        12575 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.116, whole genome shotgun sequence.
ACCESSION   DADMKF010000114 DADMKF010000000
VERSION     DADMKF010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12575
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.116"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(<1..70)
                     /locus_tag="JNC24_002962"
     CDS             complement(<1..70)
                     /locus_tag="JNC24_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HAY9159658.1"
                     /translation="MLPGCCKNGIVISKIPVMQAGLK"
     gene            689..1459
                     /locus_tag="JNC24_002963"
     CDS             689..1459
                     /locus_tag="JNC24_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HAY9159659.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNKGGWDGAVITFFASTAAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            1450..1923
                     /locus_tag="JNC24_002964"
     CDS             1450..1923
                     /locus_tag="JNC24_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HAY9159660.1"
                     /translation="MGVIEFLFALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGAI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISAVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            2031..2570
                     /gene="dnaT"
                     /locus_tag="JNC24_002965"
     CDS             2031..2570
                     /gene="dnaT"
                     /locus_tag="JNC24_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HAY9159661.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2573..3310
                     /gene="dnaC"
                     /locus_tag="JNC24_002966"
     CDS             2573..3310
                     /gene="dnaC"
                     /locus_tag="JNC24_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001731874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HAY9159662.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            3359..3853
                     /locus_tag="JNC24_002967"
     CDS             3359..3853
                     /locus_tag="JNC24_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein"
                     /protein_id="HAY9159663.1"
                     /translation="MMKTVKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            4107..6398
                     /gene="opgB"
                     /locus_tag="JNC24_002968"
     CDS             4107..6398
                     /gene="opgB"
                     /locus_tag="JNC24_002968"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HAY9159664.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIAAFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQETLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDRCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWQGKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6537..7559)
                     /locus_tag="JNC24_002969"
     CDS             complement(6537..7559)
                     /locus_tag="JNC24_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate-5-dehydrogenase"
                     /protein_id="HAY9159665.1"
                     /translation="MSTMNVLICQQPKELVWKQREIPIPGDNEALIKIKSVGICGTDI
                     HAWGGNQPFFSYPRVLGHEICGEIVGLGKNIADLKNGQQVAVIPYVACQQCPACKSGR
                     TNCCEKISVIGVHQDGGFSEYLSVPVANILPADGIDPQAAALIEPFAISAHAVRRAAI
                     APGEQVLVVGAGPIGLGAAAIAKADGAQVVVADTSPARREHVATRLELPLLDPSAEDF
                     DAQLRAQFGGSLAQKVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHK
                     KETTMMGSRNATPEDFAKVGRLMAEGKITADMMLTHRYPFATLAETYERDVINNRELI
                     KGVITF"
     gene            7698..8612
                     /locus_tag="JNC24_002970"
     CDS             7698..8612
                     /locus_tag="JNC24_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAY9159666.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSHLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPGETFSELQLARAAGVSPVVVREYLLKFGRYNLIHSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            8827..9954
                     /locus_tag="JNC24_002971"
     CDS             8827..9954
                     /locus_tag="JNC24_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010791665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY9159667.1"
                     /translation="MEKENITLDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHNFTQFVLVRIGMGIGEAPMNPCGVKVINDWFN
                     IKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYR
                     NREHVELTAVEQAYLNAGSVNARRDPLSFAEWRSLFRNRTMWGMMLGFSGINYTAWLY
                     LAWLPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSRKIC
                     IIAGMFCSAAFTLVVPQATTSMTAVLLIGMALFCIQHPAGA"
     gene            9978..10178
                     /locus_tag="JNC24_002972"
     CDS             9978..10178
                     /locus_tag="JNC24_002972"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY9159668.1"
                     /translation="MTASVGSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCV
                     TAAGALAYIFLVRQPINDPRKD"
     gene            complement(10227..11882)
                     /gene="tsr"
                     /locus_tag="JNC24_002973"
                     /pseudo
     CDS             complement(10227..11882)
                     /gene="tsr"
                     /locus_tag="JNC24_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418775.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
     gene            complement(12001..12447)
                     /gene="hpaR"
                     /locus_tag="JNC24_002974"
     CDS             complement(12001..12447)
                     /gene="hpaR"
                     /locus_tag="JNC24_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HAY9159669.1"
                     /translation="MHDSLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESP
                     SMDFHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRA
                     QTQIEEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEAIPGDNE"
BASE COUNT         3104 a         3293 c         3012 g         3166 t
ORIGIN      
        1 cttttaaccc tgcttgcata acaggtattt tactgataac aattccattt ttgcagcatc
       61 ctggcaacat atttcctcca catccactgt ctaaaaatca tgatggttta ttcaggatat
      121 tcattcctta aatgtttcca gcaaattaat agattaagcg aataaattat ccttagcatt
      181 cactttcata tcagcatact cctgctgcac aacaactcca ctgcatgtgt cagaaggtta
      241 gcatcaaatg ataacctggt gaaatagcct ggagacattt aaaatatcta cattaagttg
      301 ttaggaatac ttaatgaaaa acagaaatca cattcattta atttataatt aaatgatatc
      361 ccttcccgca gaatgaaaaa tcacacaacc aatatttatt caatgaaatc tgatggtttt
      421 aacttgttaa cattaaaaaa cgtgcccggt tatctattat cctgattcgt aaaggccaaa
      481 caataaattc attatagtga taatattcat gtgatagttt gtttgcggcg agagataatt
      541 cgctttttat caccgagctt gtcccgataa ccaggattaa tcagcgcaat tttgatctta
      601 acctaccgca aaacaaccgt gcttcatgat acaaatggat tctcattatc tgaacaatac
      661 gcagcacgtc tatgacaaag ggcgagttat gcaaactgag caacagcgag ccgtaacacg
      721 gctttgtatc cagtgtggat tatttctttt gcaacatggt gcggaaagcg cgttggttga
      781 tgagctttcc tcacgactgg gtcgggcgtt ggggatggac agcgtcgaaa gttctatctc
      841 ttcgaacgcc atagtgctga caaccattaa agatgggcaa tgcctgacat cgacacgtaa
      901 aaatcacgat cgcggcatta atatgcatgt ggtgactgaa gtccagcaca ttgtgattct
      961 ggcggagcac catctgctgg attacaaagg cgtagagaaa cgatttagcc aaattcagcc
     1021 attacgttac ccaagatggc tggtagcctt aatggttggc ctttcttgcg cctgtttctg
     1081 caaactcaat aaaggtggct gggatggtgc cgtcatcacc ttctttgcca gtacggccgc
     1141 gatgtatatc cgccagctgc tggcacaacg tcatcttcat ccgcagatca acttttgcct
     1201 caccgctttc gccgccacca ccatttccgg attgcttttg caacttccca ctttcagcaa
     1261 tacacccact atcgcgatgg ccgccagcgt tctgctgctg gtgccgggat ttccgttgat
     1321 taatgccgtc gccgatatgt ttaaaggcca catcaatacc ggactggcac gctgggcgat
     1381 cgccagtctg ctgacactgg ctacctgcgt cggcgtggtg atggcactga cgatttgggg
     1441 gctacgcgga tgggtgtgat cgagtttctg tttgcattgg cgcaggatat gatcctcgcc
     1501 gccattccag cggtcggctt tgcgatggtg ttcaacgttc ccgttcgggc attacgctgg
     1561 tgtgcgctgc ttggcgcgat tggtcatggt tcacgaatga tcttaatgac cagcgggctg
     1621 aatattgagt ggtcaacctt tatggcctcc atgctggtcg gcaccattgg tattcaatgg
     1681 tcgcgctggt atctggcgca tccgaaagtg tttaccgtgg cggccgttat ccctatgttc
     1741 ccgggcatat cggcttatac cgcaatgatt tcggcggtaa aaatcagcca gttaggttac
     1801 agcgaaccgt tgatgattac cctgttaacc aactttctta cagcttcatc gattgttggt
     1861 gcgttatcca tcggtctttc cattcctgga ttatggttgt accgcaagcg ccctcgcgta
     1921 taaaattgcc tcgcttaaag cacacggatg agagacagcc tcctctcctc cgtgtgttac
     1981 tataaaagtt atctcccttc tcgttcatcg ttccatattt gagaaacagt atgtcttcca
     2041 gagttttgac cccggacgtc gttggtattg acgccctggt acacgatcac caaaccgttc
     2101 tggcaaaagc tgaaggcggt gtggttgccg tatttgctaa caatgccccg gcgttttatg
     2161 ccgtcacgcc tgcacgcctg gctgaactgc tggcgctgga agaaaagctg gcgcgtccgg
     2221 gaagcgatgt cgctctggac gatcaactct atcaggaacc gcaagccgct cccgttgctg
     2281 tacccatggg gaaattcgcc atgtatccgg actggcaacc cgatgccgat tttatccgcc
     2341 tggcggcgct atggggtgtg gcactaagag agccggtgac caccgaagaa ctggcctcat
     2401 tcattgccta ctggcaggcg gaaggtaaag tctttcacca tgtgcagtgg caacaaaaac
     2461 tggcgcgcag cctgcaaatc ggtcgtgcca gcaacggcgg actgccgaaa cgagatgtga
     2521 atacggtcag cgaacctgac agtcaaattc caccaggatt cagagggtaa cgatgaaaaa
     2581 cgttggcgac ctgatgcaac gcctgcaaaa aatgatgcct gcccatatca aacctgcatt
     2641 caaaacgggt gaagaactac tggcctggca gaaagaacaa ggagcgatcc gctccgccgc
     2701 tctcgaacgt gaaaatcggg cgatgaaaat gcagcgcacc tttaaccgct ccggtattcg
     2761 gccactgcat cagaactgct cctttgagaa ctatcgcgtt gagtgtgaag ggcagatgaa
     2821 tgcgttaagc aaagcgcgcc agtacgtcga agagtttgac ggcaacatcg ccagcttcat
     2881 cttttctggt aagccaggaa ccggcaaaaa ccatctggcg gcggcaatct gcaacgagct
     2941 gctgctacgc ggtaaatccg tattgatcat caccgtggcc gatattatgt cggcgatgaa
     3001 agataccttc aggaatagcg gtaccagtga agaacaactg cttaacgatc tgagcaacgt
     3061 tgatctgctg gtgatcgatg agatcggcgt gcagaccgaa tcgaaatacg aaaaagtgat
     3121 catcaaccag atcgtcgatc gccgctcttc ttccaaacgc ccaaccggga tgctgaccaa
     3181 cagtaatatg gaagagatga ccaagttgct gggcgaacgc gtgatggacc gtatgcgcct
     3241 gggtaacagt ttgtgggtga tcttcaactg ggatagctac cgtagccggg taacaggtaa
     3301 agagtattaa tccccgtttc tggcggtata ctgcgacaca attccacctt gagtaatgat
     3361 gatgaaaact gttaaacatc ttctgtgctg tgccattgcc gccagcgcat taatttccac
     3421 cggggtgcat gctgcgtcct ggaaagatgc gctctccagc gccgccagcg aacttggcaa
     3481 ccaaaacagc acgacacagg aaggcggttg gtcgctcgcg tcattaacta acttgcttag
     3541 cagcgggaac caggccttaa gcgcagataa catgaacaac gccgcaggca ttctgcaata
     3601 ctgcgcgaag caaaagctgg cgtcggtaac cgatgccgaa aacatcaaga accaggtgct
     3661 ggaaaagctg ggcctgaaca gtgaagagca aaaagaagac accaactatc tggatggcat
     3721 tcagggtttg ctgaaaacca aagatggtca gcaactcaat ctggataaca tcggaacgac
     3781 tccgctggca gaaaaggtga aaaccaaagc ctgcgatctg gtgttaaagc aggggctgaa
     3841 cttcatttcc tgaaccgcga aacgccgcgt tttagctgtt acccggctga cgcggctctc
     3901 tcctgccctt ttccctgctc ctggacggtt ttccccctgt tacacgggaa gcgaccgcga
     3961 tcaaaaaagt ctgtttataa actaattcct aacaacatcg catttttttg acactagaat
     4021 tgcagcaata tggcgacgcc attacattgt gcttctcaaa aagcttaaac gttggctaaa
     4081 cggcccggtt tagtgaggat cgcccgttgt cagaactact ctctttcgcc ctttttctcg
     4141 cctctgtgct gatttacgca tggaaagcgg gacgtaacac ctggtggttt gcagccacat
     4201 taacggtgct ggggctattt gtcgttttaa atatcaccct gtttgccagc gactatttta
     4261 ctggcgatgg tattaacgac gcggttctct ataccttaac caacagcctg accggtgctg
     4321 gcgtcagcaa atacatcctg ccgggtatcg gcattgtgct ggggctgaca gcggtgttcg
     4381 gtgcgctggg ctggatcctg cgccgtcgtc gccatcatcc gcaccatttt ggttacagcc
     4441 tgctggcgct cttactggcg ctgggttcgg tggacgccag cccggcattt cgtcagataa
     4501 cggaactggt gaaatcccag tcacgcgatg gcgacccgga ctttgcggct tattataaag
     4561 agccgtcgaa aactatccct gacccgaaac tcaacctggt ttatatctac ggcgaaagtc
     4621 tcgagcggac ctattttgat aacgaagctt tcccggatct cacgcctgaa ctgggcgcgt
     4681 tgaaaaatga aggcctggat ttcagccaca cgcagcagct gccaggaacg gattacacga
     4741 ttgcgggcat ggtggcttct cagtgcggca taccgctgtt tgcccccttt gaaggcaacg
     4801 cctccgcctc tgtctccagc ttcttcccgc agaacatctg tctgggcgat atcctgaaaa
     4861 actcgggtta tcagaactat ttcgtgcagg gcgcgaatct gcgttttgcc ggtaaagatg
     4921 tgttcctgaa gtcgcatggc ttcgaccact tatacggctc agaagagctg aaaagcgtgg
     4981 tggccgaccc gcactatcgc aacgactggg gattctacga cgataccgtt ctcgatgaag
     5041 cgtggaaaaa gtttgaagag ctttcccgct caggtcagcg attctcactg tttaccctga
     5101 cagtcgatac ccatcacccg gatggtttta tctcgcgtac ctgtaaccgc aaaaaatatg
     5161 attttgacgg taagccgaac cagtcattca gcgcggtaag ttgcagccag gagaatatcg
     5221 cggcgtttat caacaaaatc aaagcgtcac cgtggtttaa agataccgtc atcgtcgtct
     5281 cttctgacca tttagcgatg aacaacacgg cgtggaaata cctcaataag caggatcgca
     5341 ataacctgtt ttttgtcatt cgtggcgaca agccgcagca agagacgctg gcagtgaagc
     5401 gtaacacgat ggataacggc gcgacggtgc tggacattct cggtggcgat aactatctcg
     5461 gacttggtcg tagcagttta tccgggcagt cgatgtcgga aatcttcctc aatatcaaag
     5521 agaaaacatt ggcgtggaag ccggatatca tccgcctgtg gaaattccct aaagagatga
     5581 aagagttcac catcgaccag cagaaaaaca tgattgcctt ctcgggtagc catttccgtt
     5641 tgccgctgct gttgcgggtt tcagacaaac gcgtggaacc gctgccggaa agcgaatact
     5701 cagcaccgct gcgtttccag ctggccgatt tcgctccacg cgacaatttc gtctgggttg
     5761 accgttgcta taagatggca caactctggg ctccggaact ggcactctcc accgactggt
     5821 gtgtctcgca agggcagctt ggcggtcagc aaattgttca gcatgttgac aaaacaacat
     5881 ggcagggcaa aacggcattt aaagatacgg tcatcgacat ggcgcgttat aaaggcaatg
     5941 tcgatacgct gaaaattgtt gataacgata ttcgctacaa agccgacagt ttcatcttca
     6001 acgtcgccgg tgcgccagaa gaggtgaaac agtttagtgg gatttcccgt ccagagtcgt
     6061 ggggccgctg gtccaacgcg cagctgggcg atgaagtaaa aatcgagtac aagcatccgc
     6121 tgccaaagaa atttgacctg gtgattaccg ccaaagcata cggcaataac gccagccgtc
     6181 ctattccggt acgcgtaggc aatgaagaac aaacccttgt gctgggcaat gaagtgacca
     6241 ccaccacact gcatttcgat aacccaaccg atgccgacac actggtaatt gtgccgccgg
     6301 aacctgtctc aaccaacgaa gggaatatcc tcggacactc gccgcgtaag ctcgggatcg
     6361 gcatggtgga aattaaagtg gtggaacgtg aaggataatt cctgactgat gtgtaggtcg
     6421 cataaggtgc gacctacaca ccttctgcgg cctacaaaac atcattgaga aacagaaaaa
     6481 aacagcgccc aacacttccc cggacgctgc atggcaactt taataatctc gccttatcag
     6541 aaagtaatta cacccttaat caactcacga ttgttaatca catcgcgctc gtaggtttct
     6601 gccagcgtgg cgaacggata gcgatgggtt aacatcatgt cagcagtgat ttttccttcc
     6661 gccatcagtc gaccgacttt agcaaaatct tccggcgtgg cgttgcggct acccatcatc
     6721 gtcgtttctt ttttatggaa ttccggatcg gagaactgca actcaccttt aaacaggccg
     6781 acaaatacca ccgtgccgcc gtgacgaatc aaattcacgg tgttattcat cgcatgttga
     6841 ttacctgtcg cgtcgatcac tttctgcgcc agcgaaccac caaactgcgc ccgtagctgc
     6901 gcgtcaaaat cttcggctga cgggtccagt aaaggtaatt ccagacgcgt tgccacatgt
     6961 tcacggcgcg ccggactggt atccgccacc accacctgtg cgccatcggc tttagcgatt
     7021 gccgccgcgc ccagaccgat tggccccgcc ccgaccacca gcacctgctc gccgggagca
     7081 atggctgcgc gacgcaccgc atgagcgcta atagcgaaag gttcaatcaa tgctgccgcc
     7141 tgcgggtcaa taccgtctgc gggcaaaatg ttcgccaccg gcacgctcaa atactcacta
     7201 aaaccgccat cctgatgcac gccaatgact gaaatttttt cacagcaatt ggtacgcccg
     7261 cttttacacg ccgggcattg ctgacaggca acataaggga tcacagcaac ttgctgacca
     7321 tttttaagat cagcaatatt tttacccagc ccaacaatct ccccacatat ttcatggcct
     7381 aaaacacgtg gataactaaa aaatggttga tttccacccc aggcatgaat atcggtaccg
     7441 caaatcccga cagacttaat ttttattaat gcttcattgt cacccggaat aggtatctcg
     7501 cgttgtttcc agactaattc tttcggctgc tggcaaatta aaacattcat cgtagacatg
     7561 attcggctct ccattttttg ttgccttaga tatcaatgaa aatgaaaaat ggcagcggaa
     7621 cggaaaatct gttttgtgaa aacacacgca taaaaatgtt ttaaatcggg ttttaatgga
     7681 ctcacaagga ggaaaatatg agtcgttcac aaaatttacg ccacaatgtg attaaccagg
     7741 tcattgatga tatggcgcgg ggtcatatcc cttccccact tccatcgcaa agtgcgctgg
     7801 cggaaatgta caacatcagc cgcaccacag tgcgtcatat tctcagccac ttacgcgaat
     7861 gcggcgtctt gacgcaggtt ggaaacgact atgttattgc gcgtaaacct gaccatgatg
     7921 acggtttcgc ctgtactacc gcttcaatga gtgaacaaaa caaagtgttt gagcaggctt
     7981 ttttcacgat gataaaccag cgccagttac gcccagggga aacgttttct gaactgcaac
     8041 tggctcgggc cgcgggtgtc agcccggttg tagtaagaga atatctttta aaattcggcc
     8101 gttataatct tattcacagc gaaaagcgcg gccagtggag catgaagcaa ttcgatcaat
     8161 cctacgccga gcagctgttt gagctacgcg aaatgctcga aacacattca cttcagcact
     8221 ttctcaacct gcctgatcat gatccccgct ggctgcaggc gaaaaccatg ctcgaacgtc
     8281 atcggctact gcgtgacaac atcggcaata gctttcgcat gttctcgcaa cttgacagag
     8341 attttcactc cttactgctt tcagctgccg acaatatctt ttttgatcaa tcgcttgaga
     8401 tcatctcagt catctttcat tttcactatc aatgggacga aagcgatctt aaacagcgca
     8461 atatcattgc cgtcgatgag catatgacca ttctcagcgc tctgatttgc cgcagtgacc
     8521 ttgatgccac ccttgcgctc cgtaaccatt tgaattcagc taaacaatca atgattcgat
     8581 ccattaatga gaacacgcgt tatgctcatt aagtacggat taaaaacagc aaactattag
     8641 cgggtgcacg gttttatcaa aagataaata ccgggaaatt cccgcttacc tatgctcaca
     8701 atcagtcgac taaaaatgtt tttgtgagcg gtagtaaagt cctaaaactt taacctgccg
     8761 ttaatgtcaa ccttgcgtaa ttcacaatta cgtaaacaat taaaattata aatacaggag
     8821 tccggcgtgg aaaaagaaaa tatcaccctc gatccgcgtt cttcatttac tccatcttcg
     8881 tcggcagata ttcccgtgcc accagatgga ttagttcaac gcagtacccg aattaaacgc
     8941 attcaaacca ccgccatgtt gttattattt tttgcggcgg taatcaatta tctcgaccgc
     9001 agttcgctgt cggtagcaaa tttaacgatt cgtgaagaat tgggattaag tgccaccgaa
     9061 atcggcgctt tgctctccgt gttttcactc gcttacggga ttgcgcaact tccttgcggc
     9121 ccactattgg atcgtaaagg cccacgcctg atgctgggac tggggatgtt cttctggtca
     9181 ctgttccagg caatgtctgg catggtgcac aactttacgc agttcgtgtt ggtgcgtatc
     9241 ggtatgggga ttggtgaagc gccgatgaac ccatgcggtg taaaagtcat taacgactgg
     9301 ttcaacatca aagagcgcgg acgcccgatg ggcttcttca acgcagcttc taccattggc
     9361 gttgccgtaa gcccaccgat tctggcggcg atgatgctgg tgatgggctg gcgcgggatg
     9421 tttattacca ttggtgtact ggggattttt ctcgccatcg gctggtatat gctctatcgc
     9481 aaccgcgagc acgtagaact gactgccgtt gaacaagctt atctcaatgc aggtagcgtc
     9541 aatgcccgcc gagatccgct cagttttgcc gaatggcgca gcctgttccg taaccgtaca
     9601 atgtggggaa tgatgctcgg attcagtggc atcaactaca ctgcgtggct gtatctggcc
     9661 tggcttcctg gttacctgca aacagcctat aacctggatt taaaaagcac agggttgatg
     9721 gcggctatcc ctttcctgtt tggggctgcc gggatgctgg tcaacggtta cgttactgac
     9781 tggctggtca aagggggaat ggctccgatt aaaagccgta agatctgcat tattgccggg
     9841 atgttctgtt ctgccgcctt tacgctggtc gtaccgcaag cgacaacatc catgacagcg
     9901 gttctgctga ttggcatggc actgttctgt attcaacatc ctgctggggc ttgatccacg
     9961 tcgcagttgc ttctcgcatg actgcgtcgg tgggcagtat ccagaacttt gccagcttca
    10021 tctgcgcctc ttttgcgccg atcattactg gttttattgt tgataccacc cactcattcc
    10081 gtctggcact aatcatctgc ggttgcgtca ccgcagcggg ggcactggcg tacatcttcc
    10141 tggttcgtca gccgatcaac gacccacgta aagattaatc tccttatgcc cgataacatt
    10201 ttgcttatcg ggcattttca tggcgattaa aatgtttccc agttctcctc gctatctgcc
    10261 acggccattt tacgcggcgc agctggcgtc acggttttta ccacagccga tgtttcacgc
    10321 tgctgttgct gaatccggaa cactgccact gcttcggtca ggcgactggc ctgctcttcc
    10381 agcgcggcgg cggcagcggc agactcttcc accagcgcgg cgttctgttg agttacccgg
    10441 tccatctcag caaccgctaa gccaacctga tcgataccac ggctctgctc atcagaagca
    10501 gaagcaattt cgcccataat gtccgtcacg cgggtcacgg cgctgacaat ctccgccatt
    10561 gtttccccgg cgctttcgac cagcgtagag ccaacatcca ctttccccac cgagtcttca
    10621 atcaggcttt taatttcacg agccgcctgg gcgctgcgct gggccagatt acgcacttct
    10681 cccgcgacca ccgcaaaacc gcgcccttgc tcacccgcac gcgcagcctc aaccgccgcg
    10741 ttcaaagcca gaatattggt ctggaaggca atgccgtcaa ttacgctgat aatatcggcg
    10801 attttctgcg aactggtgga gatatcgcgc atagtctgca ccacgttatc taccactttg
    10861 ccgccgcgtt gcgccgtttc agaagcactt aacgccagat ggctggcctg gcgcgcattt
    10921 tcggcgttct gcttcaccgt tgcggtcagt tactccatgc tggctgccgt ctcttccagc
    10981 gaagcggcct gttgctcggt gcgcgaagag agatcgttat tgccggtggc gatttcgctg
    11041 gcaccgctat agatggcatt ggccccgttg cgcacatcac cgacggtacg catcagctct
    11101 ccctgcatat ggcgcaaact ctctgccagt tgccccatct cattagagcc atccacctca
    11161 atcggtttca ccagatcgcc gcctgcaata tgacgaatgc tgtcaatcag gcgattcatt
    11221 ggcgctacca gcgaggcttt aataccgaac cagacggcga agatgaccgc cagtacgacg
    11281 atcatcacgc ccaccagaat ccacatcgcc tggctgtagg aggcattgtt atcgctgacg
    11341 gcgatatcat agagccgatc gttttgctcc atgtaagcca catactgctt ctcgaaaccg
    11401 tcctgatatc cctgggtcgg ctgatcaaag aactcgttga ttttgcctgc acctaacagt
    11461 tggatcagct ccgccagcgc attgtgataa atatcgtaat tacgtttgat ctctgccgct
    11521 gcggcggtgc tctgacgcgg gtcacgcggc aacgcttcgt aatccgccca gtttttttcc
    11581 gcctgtttca gcgaaatact ggcactctgc atcagctcag caacggttga accgctacca
    11641 atattgttct gatccatcat gtagcggata cccgcgcggt tgagggtgtt acgcgtctgc
    11701 aacaacgcga cccagctgcc attcagcgtg gattgctgct ggcgaatggt ttgtaaaaca
    11761 gtgaaatttt ctttgtcatt ctttaaggca ttaaagaaca gaccgcctga tgtcagttgt
    11821 aaaaggccaa aaacggccaa aaccagcagt aagctggtca cgatcttgat acgttttaac
    11881 atggtttctc tttcctgtgg accgatgcaa gattttcggc ctggaaagca aaaactttat
    11941 gaaaaggtgt aacccaccat ccatggaaaa accaggcgct ggttatcaga tactaaaaag
    12001 ttattcatta tcgcccggga tggcttcttg tcgggaattg cccagagcaa taaattcctc
    12061 taacagatgg gttaactgtt gcattttctc ggcagtaaac tgagcttcaa tctgccgata
    12121 ggcttcttca atctgtgtct gagcgcggtt atacagcgcc tgcccctctt tggttagcga
    12181 gatatagagc ttccgctgat cattgatcgg ctttaatcgc aacactaaac cgtcgcgttc
    12241 catccgcgtg aggatcccgg tcaggcttgg gcgcaaaatg caggcgcgat acgccagatc
    12301 gtgaaaatcc attgatgggc tttccgccag aatacgcacg atgcgccact gttgctcagt
    12361 caaattatgc cgtttaacga ttgggcgaaa ataactcatc gccgcttctc gcgcctggag
    12421 caacgcaatg gttagtgagt cgtgcattat ctttcccctg aaaaattcaa aatcattaat
    12481 atagaaacag ttaatcgtca cgttaccggg actgcgctgc ccctgcaatt cctgatttta
    12541 tcaaaattga tgatatttta ttaacttact gatat
//
LOCUS       DADMKF010000115        12487 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.117, whole genome shotgun sequence.
ACCESSION   DADMKF010000115 DADMKF010000000
VERSION     DADMKF010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12487
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.117"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            210..422
                     /gene="cspG"
                     /locus_tag="JNC24_002975"
     CDS             210..422
                     /gene="cspG"
                     /locus_tag="JNC24_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HAY9159670.1"
                     /translation="MSNKMTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRT
                     LNENQKVEFSIEQGQRGPAAANVVTL"
     gene            433..621
                     /locus_tag="JNC24_002976"
     CDS             433..621
                     /locus_tag="JNC24_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAY9159671.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHAPLDFTK"
     gene            596..826
                     /gene="ymcE"
                     /locus_tag="JNC24_002977"
     CDS             596..826
                     /gene="ymcE"
                     /locus_tag="JNC24_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HAY9159672.1"
                     /translation="MRRWISQNNIRLPRGAFFISALFFFNAVCIVSDNLLIIESFGEM
                     AYNISYLTRVPGTNTLLACCCLLRPEEVNSEY"
     gene            816..989
                     /locus_tag="JNC24_002978"
     CDS             816..989
                     /locus_tag="JNC24_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HAY9159673.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFTAREKM
                     TGLESYDVKIKIM"
     gene            complement(1038..2111)
                     /locus_tag="JNC24_002979"
                     /pseudo
     CDS             complement(1038..2111)
                     /locus_tag="JNC24_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415512.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
     gene            complement(2183..4927)
                     /gene="torS"
                     /locus_tag="JNC24_002980"
     CDS             complement(2183..4927)
                     /gene="torS"
                     /locus_tag="JNC24_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
                     /protein_id="HAY9159674.1"
                     /translation="MNLTLTRRLWMGFALMALLTLTSTLVGWYNLRFISQVEKDNTQA
                     LIPTMNMARQLSEASAWELFAAQNLTSADNEKMWQAQGRMLTAQSLKINALLQALREQ
                     GFDTTAIEQQEQEISRSLRQQGELVGRRLQLRQQQQQLSQQIVAAADEIARLAQGQAN
                     NATTSAGATQAGIYDLIEQDQRQAAESALDRLIDIDLEYVNQMNELRLSALRVQQMVM
                     NLGLEQIQKNAPTLEKQLNNAVKILQRRQIRIEDPGVRAQVATTLTTVSQYSDLLALF
                     QQDSEISNHLQTLAQNNIAQFAQFSSEVSQLVDTIELRNQHGLAHQEKASARGQYSLL
                     LLGMVSLCALILILWRVVYRSVTRPLAEQTQALQRLLDGDIDSPFPETAGVRELDTIG
                     QLMGAFRSSVHALNRHREQLAAQVKARTAELQELVIEHRQARAEAEKASQAKSAFLAA
                     MSHEIRTPLYGILGTAQLLADNPALNAQRDDLRAITDSGESLLTILNDILDYSAIEAG
                     GKNVSVSDEPFEPRPLLESTLQLMSGRVKGRPIRLATAIADDMPCALMGDPRRIRQVI
                     TNLLSNALRFTDEGYIILRSRTDGEQWLVEVEDSGCGIDPAKLAEIFQPFVQVSGKRG
                     GTGLGLTISSRLAQAMGGELSATSTPEVGSCFCLRLPLRVATAPVPKTVNQAVRLDGL
                     RLLLIEDNPLTQRITVEMLNTSGAQVVAVGNAAQALETLQNSEPFAAALVDFDLPDVD
                     GITLARQLAQQYPSLVLIGFSAHVIDETLRQRTSSLFRGIIPKPVPREVLGQLLAHYL
                     QLQVNNDQSLDVSQLNEDAQLMGTEKIHEWLVLFTQHALPLLDEIDIARASQDSEKIK
                     RAAHQLKSSCLSLGMRIASQLCAQLEQQPLSAPLPHEEITRSVAALEAWLHKKDLNAI
                     "
     gene            5010..6038
                     /gene="torT"
                     /locus_tag="JNC24_002981"
     CDS             5010..6038
                     /gene="torT"
                     /locus_tag="JNC24_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HAY9159675.1"
                     /translation="MRVLLFLLLSLFMLPAFSADNLLRWHDAQHFTVQASMPLKAKRA
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVASLPVIELVNAIDAPQVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(6011..6703)
                     /gene="torR"
                     /locus_tag="JNC24_002982"
     CDS             complement(6011..6703)
                     /gene="torR"
                     /locus_tag="JNC24_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HAY9159676.1"
                     /translation="MPHHIVIVEDEPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDNCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            6823..7995
                     /gene="torC"
                     /locus_tag="JNC24_002983"
     CDS             6823..7995
                     /gene="torC"
                     /locus_tag="JNC24_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HAY9159677.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHARQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEIAHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKDEK"
     gene            7995..10952
                     /gene="torA"
                     /locus_tag="JNC24_002984"
                     /pseudo
     CDS             7995..10952
                     /gene="torA"
                     /locus_tag="JNC24_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
     gene            complement(11045..11350)
                     /gene="cbpM"
                     /locus_tag="JNC24_002985"
     CDS             complement(11045..11350)
                     /gene="cbpM"
                     /locus_tag="JNC24_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HAY9159678.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(11350..12270)
                     /gene="cbpA"
                     /locus_tag="JNC24_002986"
     CDS             complement(11350..12270)
                     /gene="cbpA"
                     /locus_tag="JNC24_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HAY9159679.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNHQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPAARGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGAKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
BASE COUNT         3011 a         3204 c         3269 g         3003 t
ORIGIN      
        1 ggacggctaa aataaaattt gcttaatctc aattatcatg cgttaatagc tgcgtcggtt
       61 tgaaagacag acagcataca aagtagttta ctaaagcagt tctcattatc aggcattatc
      121 cccttctttt gagtctctct cctgaacact aagtagtttc tgtattaaag ccctgtttgc
      181 cgaaaggccc aaaatgaagg aagtaaaata tgtctaataa aatgactggt ttagtaaaat
      241 ggtttaacgc agataaaggt tttggcttta tcactcctga tgatggcagc aaagacgttt
      301 tcgtccattt caccgccatc cagagcaatg aattccgcac gctgaacgaa aatcagaaag
      361 ttgaattttc tattgagcag gggcaacgtg gccccgcggc agcgaacgtt gttacgctct
      421 aaggttgccg ttattactca acatctccat ttccgctgtc catgttgtca tggttcacag
      481 taccgcacat cggcattcga tgtgacggag cgaaaccctt tgggcgctaa gtgtattttt
      541 tgtaaatcga cgatgatcac ctttgataac gtcgcgctgc aaatacgcac tgaccatgcg
      601 ccgctggatt tcacaaaata atatcaggct ccctcgtgga gcctttttta tatctgcctt
      661 atttttcttc aacgctgtat gtatagtaag cgataacctg ttgattattg aatctttcgg
      721 ggagatggct tataacattt cttacctgac cagggtaccg ggaaccaaca ccttactggc
      781 gtgttgctgt cttttaagac cagaagaggt taacagtgaa tattgaagag ttaaaaaaac
      841 aagccgaaac ggaaatcgcc gactttatcg cgcaaaaaat cgccgagctg aacaagaata
      901 cagggaaaga agtctctgaa attcgcttca ccgcacgaga aaaaatgacc gggcttgaaa
      961 gttatgatgt caaaatcaaa ataatgtgat tttgtgaaca tcaccccgtg cgaggtgatg
     1021 ttccgcttgt tgctaattta gtgaccaatc attggcgctt gtggaattaa gcgtcggtac
     1081 aattcctccg gcaccgggct ttgccatact cccgcataca ttgcgtaacc aatcaccgcg
     1141 aacataatcc tcagaaccag tagcgtcatt aaccagccag acaacgcaaa agctttttta
     1201 tttgccgcag gtttttgcag tgaaaaggtc aatgttgagg ctaccggaca tgactctacg
     1261 caagtcatac agccggtaca ttccactgtt cgtacctgaa ttaatttatc taccgggatc
     1321 cgtgatgggc aattttttgc gcatttgcca cagccgatac aactttcggc attgcgacga
     1381 atcttaaacg gcgataatag cgaaaccacg cccatcagcg cgccatatgg gcaaagataa
     1441 cgacactagg catggcgaat aaacaggctg gcaatcagca aaacggtcac gctgattaat
     1501 gtcgcggtcc ccatatgacg aaagaaatcg agcattttta cgtccatcac cacgctgtag
     1561 ggcgacaaca taaaatagtg aatcgcctga gcgggcatca ataacgcgat atagagaaaa
     1621 aaactcaaca gcaaatactt cacgccgcgc agaggaatat ccagccagcg gggaaggaca
     1681 cattgccgac caaacagttt gttaccgaga tcgccgatta attcagaaag cgtaccaacc
     1741 gggcataacc atgagcaaaa ggcctttttg agtaatagac tgatgacgat aaaagcgacc
     1801 aataacagca tcgcggcggt gtggacggac ggtaactgac ctgttgtaag gctatatttc
     1861 agattcatca gaccggcaat cggtagccag ccttcgatac ctcccggtct ggcgacaaat
     1921 gtcgtgctac ttgccgtttc gtaatagcgc acccaatacc agaacgtgat ggcaatataa
     1981 atattcattg ccaacagtaa taattgcgtc gctttacgcc aggtcgtggc attgcgccag
     2041 tcattccacg gtaatttgcc gcccgtcgtg cctggccgcc gctgccagcg ggttcttttt
     2101 ttctctgcca tgattttgcc agtccgttaa gttgcatacc aaatgccact attctagttg
     2161 ttcttaactg gctgatattg attcaaatcg cgttcaggtc tttcttatgc aaccatgctt
     2221 ccagagcggc aacactgcgt gtaatttctt cgtgtggaag gggggcagat aatggctgct
     2281 gctccagttg tgcgcatagc tggctggcga tacgcattcc caaactcaag caactgcttt
     2341 ttagctgatg cgcggcacgc tttattttct cgctatcctg actggcgcgg gcaatgtcga
     2401 tttcatcgag aagcggcagg gcatgttgtg taaataatac cagccattcg tggatcttct
     2461 ccgtccccat taactgagca tcttcattga gttgcgatac atccagcgat tgatcgttat
     2521 tgacttgcag ttggagatag tgcgccagta actgaccgag cacttcacgc ggcaccggtt
     2581 tagggataat cccgcggaat aatgaactgg tacgctggcg cagcgtttcg tcaatgacat
     2641 gggcgctaaa gccaatcaaa accagcgacg gatattgctg tgccagttgt cgggcaagcg
     2701 taatgccgtc gacatccggc agatcaaaat ccaccagtgc ggcagcaaac ggttcgctat
     2761 tttgcagtgt ctctaaagcc tgcgcggcat tgccaacagc aacaacctgc gcaccactgg
     2821 tgttcagcat ctcaacggta attcgctggg ttagcgggtt atcttcaatt aacagcaaac
     2881 gtaaaccgtc aagacgcacc gcctgattga ctgtttttgg cacgggtgcc gtggcaacac
     2941 gtaacggcaa gcgtaaacaa aaacagcttc caacctccgg cgtgctggtg gcgctcagtt
     3001 cgccgcccat cgcctgggcc agacggctac tgatagtcag ccccagcccg gtgccgccgc
     3061 gtttgccgct tacctgcaca aatggctgga agatttctgc cagtttcgcg ggatcaatac
     3121 cgcagccgct gtcttccact tcgaccagcc attgctcgcc atcagtgcga ctacgcagga
     3181 taatgtaccc ttcgtcagta aaacgcaggg cgttgctcaa caggttggtt ataacctgac
     3241 gaatacgtcg tggatcgccc attaacgcgc acggcatatc atcggcaatt gccgttgcca
     3301 ggcgaatcgg gcgacctttc acccgtccgc tcattaattg cagggtactt tccagcagcg
     3361 ggcgcggttc aaagggctca tcgctgaccg aaacattctt gccacctgct tcgatagcgg
     3421 aataatcgag aatatcgttg aggatggtca gcaacgattc accagagtca gtaattgccc
     3481 gcaaatcatc acgctgggcg ttaagtgcgg ggttatctgc cagtagctgt gcggtgccga
     3541 gaataccgta cagcggtgtg cggatctcat ggctcatcgc cgccagaaac gccgattttg
     3601 cctggctggc tttttctgct tccgcccgtg cctgtcggtg ttctatcact agttcctgca
     3661 attcagctgt acgcgctttg acctgcgccg ccagctgttc acggtggcga ttcagtgcat
     3721 gaacactgct gcgaaacgca cccatcagct gcccgatggt gtccagctcc cgtacgccag
     3781 cggtttccgg gaaaggggag tcaatatcac cgtccagcag ccgttgcagc gcctgcgttt
     3841 gttcggcaag tggacgcgtg actgagcgat aaaccacgcg ccagaggatc agaatcagtg
     3901 cgcaaagtga aaccatcccc agcaataaca ggctgtattg cccgcgtgca ctggcttttt
     3961 cctgatgcgc cagtccgtgc tgattacgca gctcaatggt gtcgaccagc tgactgactt
     4021 cgctactaaa ctgcgcgaac tgggcgatat tattttgggc gagagtttgc aggtgattgc
     4081 tgatttcact gtcctgctgg aacagcgcca acagatcgct atattggcta acggtagtta
     4141 acgttgttgc gacctgcgca cgaactcccg gatcttcaat gcgtatttgc cgacgttgca
     4201 gaattttcac cgcattattg agctgctttt ccagcgttgg tgcatttttc tggatctgct
     4261 ccagccccag attcatcacc atttgctgca cccgcagagc gctaaggcgc agttcattca
     4321 tctggttaac atactcaaga tcgatatcaa tcagccgatc gagtgcactt tcagcagcct
     4381 gacgctgatc ttgttcgatc aaatcgtaaa tcccggcctg ggtcgctcca gcggaagttg
     4441 tcgcattatt cgcctgacct tgcgccaggc gtgcgatctc atcggcggca gcgactatct
     4501 gctgactgag ttgctgttgt tgctggcgta gttgcagacg ccgccccacc agttcccctt
     4561 gctgacgtaa cgaacgggag atctcctgct cctgttgttc aatagcggtg gtatcaaaac
     4621 cttgttcccg taacgcttgc agcaacgcat taatcttcag gctttgtgcg gtgagcattc
     4681 gcccctgcgc ctgccacatc ttttcgttat cggcactggt caggttctgc gcggcgaaaa
     4741 gttcccaggc gctggcttcg ctcaactggc gcgccatatt catggtagga atcaatgcct
     4801 gagtattgtc tttttccacc tggctgataa agcgcaggtt gtaccatccc accagggtac
     4861 tggtcagggt taacagcgcc atcagggcaa agcccatcca gagtcttcgg gtcagggtta
     4921 aattcacggt cggtgcactt taggtgaaaa atgttgagtc gcaaagcgga atgcatctag
     4981 cataaagcct tattattgat gaggctatca tgcgcgtact gctattttta cttctttccc
     5041 ttttcatgtt gccggcattt tcggctgata acctgttgcg ctggcatgat gcgcagcatt
     5101 tcacggtgca agcctctatg ccgcttaaag ccaaacgcgc atggaaactg tgcgcgcttt
     5161 atcccagcct gaaagattca tactggttat cgttgaacta tggtatgcag gaggcagctc
     5221 gccgttatgg tgtggattta aaagtgctgg aggcaggcgg ctacagccag ttggctaccc
     5281 agcaagcaca aatcgaccag tgtaaacagt ggggcgcaga ggccatttta ctcggtagta
     5341 gcacgacctc atttcccgat ctgcaaaagc aggtagcaag tctgccggtg atcgaactgg
     5401 taaatgctat tgatgctccc caggtgaaaa gccgcgttgg tgtaccctgg tttcagatgg
     5461 gctatcaacc tgggcgatat ttggtgcaat gggcgcacgg taaaccactg aacgtgctgt
     5521 tgatgcccgg acccgataat gccgggggca gtaaagagat ggtcgagggt tttcgcgcag
     5581 ccattgccgg aagcccggtg cgtattgttg atattgcgct tggtgataac gatattgaaa
     5641 tccagcgtaa cctgttgcag gagatgctgg aacgccaccc agaaatcgac gtcgttgccg
     5701 gaacggccat tgcggcagag gcggcaatgg gtgaagggcg taacctgaaa acgccgctta
     5761 ccgtggtgtc gttttatctt tcacatcagg tgtatcgcgg gctgaagcgg ggaagagtga
     5821 ttatggctgc cagcgatcaa atggtctggc agggggaact ggcggttgag caggccatca
     5881 ggcaattaca ggggcaatcg gtgtctgata atgtcagccc accgatttta gttctgacgc
     5941 cgaaaaatgc cgaccgtgaa catattcgcc gctcgctgtc accaggggga tttcgtccgg
     6001 tctattttta tcagcacaca tcagcggcta agaaataacc ttcaccatgt tgcgtcacca
     6061 gtaaatccgc gctgagttta tgacgtaaac gacgaattaa cacatcgacg gtgcgcaggt
     6121 cagggttttc cacccgacgc gcagaaagca tacgtagcag acgttcacgg ctgagaattt
     6181 cgcccggatt cgtcacaaat gccaccaaca tttcatactc tgcgcgggtc agtttaatcg
     6241 gctcgccatc ccgctccagc gtatggcgcg acacattcag gcaataaccg gcaaagcgat
     6301 agcagttgtc ctgagtgtgc ggttgagctt gtcgcgcgag gtcgattcgc cagagcagat
     6361 ttttcacccg tactaccagt tcgcgcagtt ccagcggttt ggtgacgtaa tcgtctgcgc
     6421 ccatttccag cccaacaata cggtcaatcc gatcgctgcg tccggtaacc agaataatcc
     6481 ccaccgttga gcgttctcgc agggcgcggg ttaacatcag gccattttca tcgggtaagt
     6541 tgatatccag cagaattaaa tctaccggct gattctgcat aatttcccgc agcccggcac
     6601 cgctcgctgt aacggaaacg gtatacccct cctgagtgaa gtaagattgt aatcgcgcct
     6661 gggtaaccgg ctcatcttca acaataacaa tgtgatgtgg catcagaggg ttttactcat
     6721 tctgttcata ttctgccgta agccgttcat cctgaccagt gccgctgttc atatttgctc
     6781 attaagatcg cttcactaaa ccataatttt acaggggcta ttatgcggaa actctggaac
     6841 gcgctacgcc gacccagtgc tcgttggtcg gtactggcgc tggtcgctat tgggattgtg
     6901 attggcattg cgctgattgt attgccacac gttgggatca aagtcaccag cacaaccgaa
     6961 ttttgtgtca gttgccacag tatgcaaccg gtgtatgaag aatataaaca gtcggtgcat
     7021 tttcagaacg cctctggcgt gcgagctgaa tgccatgact gtcatatccc gccggatatt
     7081 ccaggcatgg tgaagcgcaa actggaagcg agcaacgaca tttaccagac ctttattgcc
     7141 cactccattg atacacctga aaaattcgaa gccaaacgcg cggaacttgc cgagcgtgaa
     7201 tgggcgcgaa tgaaagaaaa caactcggca acctgtcgct cctgccataa ctacgatgcg
     7261 atggatcatg ccaggcagca tcctgaagcg gcacgtcaga tgaaggtggc agcgaaagat
     7321 aatcaatcct gcatcgactg tcataaaggt attgcccacc agttaccgga tatgagtagc
     7381 ggcttccgta agcagttcga tgagctgcgc gccagtgcta atgacagtgg tgacacgctg
     7441 tactctattg atattaagcc aatttatgcg gcgaaaggcg ataaagaagc ctctggttct
     7501 ctgctgcctg cttcggaagt gaaagtcctt aaacgtgacg gcgactggct gcaaattgaa
     7561 attaccggct ggacggaaag cgccggacgt cagcgtgtac tcacccaatt cccaggtaaa
     7621 cgcatctttg ttgcctcgat tcgtggtgat gtgcagcagc aggtgaaaac gctggagaaa
     7681 accaccgttg ccgacaccaa taccgagtgg agcaagttgc aggccaccgc gtggatgaag
     7741 aaaggcgaca tggtgaacga tatcaaaccg atctgggcct atgcggattc gttgtacaac
     7801 ggcacctgta accagtgcca cggcgcaccg gaaatcgccc actttgacgc taacggttgg
     7861 atcggcacgc tcaacggcat gattggcttt accagcctcg ataaacgtga agaacgcacc
     7921 ttgttgaaat atctgcaaat gaatgcgtct gacaccgcag gtaaggctca cggcgataag
     7981 aaggacgaaa aataatgaac aataacgatc tctttcaggc atcacgtcgg cgttttctgg
     8041 cacaactcgg cggcttaacc gtcgccggga tgctggggcc gtcattgtta acgccgcgcc
     8101 gtgcgactgc ggcgcaagcg gcgactgagg ctgtcatctc gaaagagggc attcttaccg
     8161 ggtcgcactg gggggctatc cgcgcgacgg tgaaggatgg tcgctttgtg gcggcaaaac
     8221 cgttcgaact ggataaatat ccgtcgaaaa tgattgccgg attgccggat cacgtacaca
     8281 acgcggcgcg tattcgttat ccgatggtac gcgtggactg gctgcgtaag cgccatctca
     8341 gcgatacctc ccagcgcggt gataaccgtt ttgtgcgcgt gagctgggat gaagccctcg
     8401 acatgttcta tgaagaactg gaacgcgtac agaaaactca cgggccgagt gccttgctga
     8461 ccgccagtgg ttggcaatcg acggggatgt tccataacgc ttcggggatg ctggcgaaag
     8521 ctattgcctt gcatggtaat agcgttggta cgggcggaga ttactctacc ggtgctgcgc
     8581 aggtgatcct gccgcgcgtg gttggctcaa tggaagtgta tgaacagcaa acctcctggc
     8641 cgctggtatt gcagaacagc aaaaccattg tgctgtgggg ctctgatttg ctgaaaaacc
     8701 agcaagcgaa ctggtggtgc ccggatcacg atgtttatga atattacgcg cagctaaaag
     8761 cgaaagtcgc cgccggtgaa attgaggtca tcagcatcga tccggttgtc acatccaccc
     8821 atgagtatct ggggcgcgag catgtgaagc acattgcggt taacccgcaa actgatgtgc
     8881 cgctgcaact ggcgctggcg tatacgctgt acagtgaaaa cctgtacgac aaaaacttcc
     8941 tcgctaacta ctgtgtgggt tttgagcagt tcctgccgta cctgctgggt gagaaagacg
     9001 gtcagccgaa agatgccgca taggctgaaa aactgaccgg cattgatgcc gaaaccattc
     9061 gtgggctggc gcggcagatg gcggcgaaca gaacgcaaat tattgctggc tggtgcgtgc
     9121 agcgtatgca gcacggtgaa cagtgggcgt ggatgattgt ggttctggcg gcgatgctgg
     9181 ggcaaattgg cctgccaggt ggtggctttg gttttggctg gcactataac ggcgcaggca
     9241 cgccggggcg taaaggcgtt attctgagtg gtttctccgg ctctacgtcg attccgcctg
     9301 ttcacgacaa cagtgattac aaaggctaca gcagcaccat tccgattgcc cgttttatcg
     9361 atgcgatcct cgaaccgggg aaagtgatca actggaacgg taaatcggta aaactgccgc
     9421 cgctgaaaat gtgtattttt gctggaacta acccattcca tcgccatcag cagatcaacc
     9481 gcattattga aggctggcgc aagctggaaa cggttatcgc catagataac cagtggacct
     9541 caacctgccg ctttgccgat atcgtgctgc ctgcgaccac gcagtttgag cgtaacgatc
     9601 tcgaccagta cggcaaccac tccaaccgtg gcattatcgc catgaaacag gtggtgccgc
     9661 cgcagttcga ggcgcgcaac gactttgata ttttccgcga gctgtgccgt cgctttaatc
     9721 gcgaagaagc ctttaccgaa gggctggacg aaatgggctg gctgaaacgc atctggcagg
     9781 aaggtgtaca acaaggcaaa ggacgcggcg ttcatctgcc agcgtttgat gacttctgga
     9841 ataacaaaga gtacgtcgag tttgaccatc cgcagatgtt tgttcgccac caggcattcc
     9901 gcgaagatcc ggatctcgaa ccgctgggca cgccgagtgg cctgattgag atctactcga
     9961 aaaccatcgc cgatatgaac tacgacgatt gtcaggggca tccgatgtgg tttgagaaaa
    10021 tcgaacgctc ccacggtggg cccggctcgc agaagtatcc gttgcatctg caatctgtgc
    10081 atccggattt ccgacttcac tcgcagttat gtgagtcgga aactctgcgt cagcaatata
    10141 cggtagcggg taaagagcca gtattcatta acccgcagga tgccagcgcg cgcggtattc
    10201 gtaacggtga tgtggtacgc gtctttaacg ctcgcggtca ggtgttggca ggggcagtgg
    10261 tttctgaccg ctatgcaccc ggcgtggcac gaattcacga aggggcatgg tacgatccag
    10321 ataaaggcgg cgagcctggt gcgctgtgca aatacggtaa ccccaacgtg ttgaccatcg
    10381 acatcggtac atcgcagctg gcgcagttgt tctcccgtga actggacgat gaacaactga
    10441 cgcaaatcgc cagtgcgcag atggctgaat ggttttcgtt gctgaaaagc gaaccgccgc
    10501 tcactgcggc ggtgaatgcg ctggaaaacc gtattgccgc tctgacagta cgtgacgatg
    10561 cccgtctgga actggccgct gacttttgcg gcctgtttct gatgaccgac aaacaagcgg
    10621 cgctgccgta tgcatcggcc tacaaacagg acgagcaaga gattaaacgc ttgttagttg
    10681 aggcagggat ggaaaccagt ggcaatttca acgaatcggc agatcatctg gcgatctatc
    10741 tcgagttgtt gagtcatctg catttttcgc tgggagaggg gaccgttcct gcgcgaagaa
    10801 tcgacagttt gcggcaaaaa acactgacgg cgctgcggca atggttacca gagtttgctg
    10861 cgcgttgtcg acagtatgac agctttggtt tttacgcggc actaagccag ttattgctgg
    10921 tgttagtgga gtgcgaccac caaaacagat aacgtcgttt atgcgcctga aaagacgcgt
    10981 ttagcgtcgc atcaggcatt atggcgcagt tgccggatgc gtcatcacat ccggcaaaca
    11041 cacatcacgg atgagctaca aaccgggaaa gccgctggcg tagcaggcgg ttttcctgct
    11101 tcaggtgcgc aatatcatcc attaacgtca gcgccaccgc gatccccggc cagtccagag
    11161 ccagttcatg acgcaggcgt accgcgcgtt gcaccacaat ggcggcatgg tcgtcaaata
    11221 cccaggttgt ttcctggatc tcacgcggtt caaccacccc caaaccgaca atttcattca
    11281 actcctcttc agagatgccg gtatgcaggc aaaattcggt aatagtaaaa gtcaccgtaa
    11341 cattagccat catgctttcc cccaatcttt acgtggatca aaagacgact gggcgtctgc
    11401 cagttgctgc cacagcgcgg cagtgttttc atccggtttc ggcggcatca cgattttcag
    11461 taccgcatac agatcgccgg tctgtttttt gctcaccaga cctttgcctt taacgcgcaa
    11521 tcgttgcccg gcctggctgc ctggcgggat agtcagcaaa atgctttctt tcagtgttgg
    11581 aacggtgact ttagcaccca gcgccgcttc ccacgggcta accggcacca caatttccag
    11641 atcctggccg acaatatcga acagcggatg tggcgcaata tgaatcacca gccacaaatc
    11701 gccatttgga ccaccgtttt cgcccggcgt cccctggcct ttcagacgga tgcgttgacc
    11761 attgccgacg cccgccggga tcttcacatt cagcgttttc gggatttcct gttcgatcat
    11821 gccaaaggcg ttataaaccg gcaggttata gctgatggta cgcttatgct cagtcagcgt
    11881 ttcttcgagg aataccgcca cttcgatttc aatatcgtgg ccgcgtgcgg cggggcgttg
    11941 acggctctgg cgggcatgct gaccgaaaat tgacgagaag atatcgtcaa aatcttcggc
    12001 attaaaactc tgaccgtcgc catgatggaa ctgatggtta aattgcggat cgttgcgatg
    12061 ttgccacatc tgatcatact cagcgcgacg ttgttcatca cttaacactt cccaggcttc
    12121 agcgacctct ttgaagcggg cttcggcatc cggttctttg ctgacatcag gatggtattt
    12181 acgggccagt cgacgatagg cggtcttgat tgtcttgaga tcgtccgtcg gtttcacgcc
    12241 catgatggcg taataatcct ttaattccat agcgttatct cgcgtaaatc aacacaaatt
    12301 gaaggaaccc ctgtaaggta actcctataa gtgtagggta atcctcaaaa tttcatatgc
    12361 caacacagaa tatgttattg aaatcatcgc ggagaggagg tcgccatcaa gatgggttgc
    12421 tgaacatatt ttaaacaggt gaaaaagggt gagcgatttt tgatagttga accaggcact
    12481 ttaagtt
//
LOCUS       DADMKF010000116        12359 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.118, whole genome shotgun sequence.
ACCESSION   DADMKF010000116 DADMKF010000000
VERSION     DADMKF010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12359
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.118"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(121..330)
                     /gene="flhB"
                     /locus_tag="JNC24_002987"
                     /pseudo
     CDS             complement(121..330)
                     /gene="flhB"
                     /locus_tag="JNC24_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310617.1"
                     /note="membrane protein responsible for substrate
                     specificity switching from rod/hook-type export to
                     filament-type export; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
     gene            complement(532..1176)
                     /gene="cheZ"
                     /locus_tag="JNC24_002988"
     CDS             complement(532..1176)
                     /gene="cheZ"
                     /locus_tag="JNC24_002988"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HAY9159680.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTRMLRDSLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGLVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKVGVVASQDQVDDLLDSLGF"
     gene            complement(1187..1576)
                     /gene="cheY"
                     /locus_tag="JNC24_002989"
     CDS             complement(1187..1576)
                     /gene="cheY"
                     /locus_tag="JNC24_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheY"
                     /protein_id="HAY9159681.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(1591..2640)
                     /locus_tag="JNC24_002990"
     CDS             complement(1591..2640)
                     /locus_tag="JNC24_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HAY9159682.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLAKISAGQAIRI"
     gene            complement(2643..3503)
                     /gene="cheR"
                     /locus_tag="JNC24_002991"
     CDS             complement(2643..3503)
                     /gene="cheR"
                     /locus_tag="JNC24_002991"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HAY9159683.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHAHRRSGEYRVWSAAASTGEEPYSIAMTLADTLGTAPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQNTQQEILRRFVPLLKPDGLLFAGYSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(3522..5123)
                     /gene="tap"
                     /locus_tag="JNC24_002992"
     CDS             complement(3522..5123)
                     /gene="tap"
                     /locus_tag="JNC24_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HAY9159684.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNQVE
                     QSNQQRAALAQTRAVMLQASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNDLEHQATWLESNQLSDFLTAPVQGSQNAFD
                     VNFEAWQLEINHVLEAASAQSQRNYQISTLVFISMIIVAAIYISSALWWTRKMIVQPL
                     AIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSQEMHIG
                     IAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAQ
                     AGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMIDIVSSVTRVND
                     IMGEIASASEEQQRGIEQVAQAVSQMDQVTQQNASLVEEAAVATEQLANQADHLSSRV
                     AVFTLEEHEVARHESAQLQIAPVVS"
     gene            complement(5169..6830)
                     /gene="tar"
                     /locus_tag="JNC24_002993"
     CDS             complement(5169..6830)
                     /gene="tar"
                     /locus_tag="JNC24_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HAY9159685.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNIDEKYKNYYTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLT
                     PLAKIIAHIREIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSAQAAKEIKALIEDSVARVDTGSVLVESAGETMNNIVNAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRIAEQDPNWETF"
     gene            complement(6975..7478)
                     /gene="cheW"
                     /locus_tag="JNC24_002994"
     CDS             complement(6975..7478)
                     /gene="cheW"
                     /locus_tag="JNC24_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HAY9159686.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(7499..9463)
                     /gene="cheA"
                     /locus_tag="JNC24_002995"
     CDS             complement(7499..9463)
                     /gene="cheA"
                     /locus_tag="JNC24_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HAY9159687.1"
                     /translation="MSMDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASFDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQSPR
                     RIILSRLKAGEVDLLEEELGHLTTLTDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKISTPPVLKLAAEQAPTGRVEREKTTRSNESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSMAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVGDEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(9468..10394)
                     /gene="motB"
                     /locus_tag="JNC24_002996"
     CDS             complement(9468..10394)
                     /gene="motB"
                     /locus_tag="JNC24_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HAY9159688.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEDLKKRMEQSRLRKLRGDLDQLIESDPKLRALRPHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRVIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(10391..11278)
                     /gene="motA"
                     /locus_tag="JNC24_002997"
     CDS             complement(10391..11278)
                     /gene="motA"
                     /locus_tag="JNC24_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HAY9159689.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGGVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(11404..11982)
                     /gene="flhC"
                     /locus_tag="JNC24_002998"
     CDS             complement(11404..11982)
                     /gene="flhC"
                     /locus_tag="JNC24_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HAY9159690.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(11985..12335)
                     /gene="flhD"
                     /locus_tag="JNC24_002999"
     CDS             complement(11985..12335)
                     /gene="flhD"
                     /locus_tag="JNC24_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HAY9159691.1"
                     /translation="MHTSELLKHIYDINLSYLLLAQRLIVQDKASAMFRLGINEEMAT
                     TLAALTLPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGIMLSTRLLN
                     DVNQPEEALRKKRA"
BASE COUNT         2831 a         3451 c         3021 g         3056 t
ORIGIN      
        1 gagtcggcga attacatagg ggctcgcagc gttacggctg caataaagcc ggatataaag
       61 ctgcaaccta cccgtcatgt caaaacaatg gatgccttgc aaacgggatg cgttcatata
      121 aatactgtga tcaaaatgca gcccagcgga gagcatgccc gacaatcgac gggccagcga
      181 cacaccgcca aaccagataa cacacacgcc cactaacaaa atcagcaacg aggtcagttc
      241 acgggaacgc gggatttgcc cctcttcccg cgctttttct agtcggtgag gtgtgggggc
      301 ttctgttttg tcgtcgctct cgtcagacac gtcgccaatc ctggaaagag tattaagctt
      361 aaatgatgcc agagcgcaaa gcgttcaatg gtttgagtaa ggggcaaaac aggcgggatt
      421 tagggctttt gctgccacac atcaagcata gtgtgcgttt gtcggatgcg gcgtcatcgc
      481 cttatcagac cgcctgatat gacgtggtca cgccacatca ggcaatacaa atcaaaatcc
      541 aagactatcc aacaaatcgt ccacctgatc ctgactggct accacgccga ctttgctggt
      601 atcgacctga ggtccattaa gcaaactctg gttttcacgt tttggacgcg actcctgttc
      661 cgggatgttt tccaacagca ccatcagcaa ctggcgttcg atctcctgaa tgacatccat
      721 catccgctta atgaccagcc cggtgagatc ctgaaaatcc tgcgccatca tgatttccag
      781 cagttgcgcg ttagtaaagc tggtatgcgc gggtacatct gccagaaatt gtcgtgtatc
      841 tgttaccagt tcacgggcat cggcaaggtc aatcggatcg gcaaaccagt catcccaacg
      901 ttgggttaac gcttttgctg atttctccat ttgatcctga tgcggttgtg acgcctcaac
      961 actgttcagc gcccgctccg cagcctgggc ggtcatctgc acaacatagt acaaacgatc
     1021 gcgcgcatcg gggatggctt ccgccgcttc ggcaatggcc tgatccagcc ccagttcccg
     1081 caaactgtcg cgcagcatac gcgtcaggct gccgatgcgc gcaatgatat cgccagctga
     1141 atgctcgtca gcaggtttga ttgatggttg catcatagtc gcatcctcac atgcccagtt
     1201 tctcaaagat tttgttgagt ttttcctcca gcgtcgcggc ggtaaatggc ttcaccacat
     1261 agccactggc ccccgcttgc gccgcagcaa tgatgttctc tttcttcgct tctgcagtca
     1321 ccattaacac tggcaatgcc gacatcgcgc catccgcacg aattgttttc agcaattcca
     1381 ggccatccat attgggcatg ttccagtcgg agataacaaa tccataaccg cctgcctgca
     1441 acttattgag agcgtcgacg ccatcttccg cttcctcaac attattgaat cccagctctt
     1501 tcagcaggtt acgcactatg cgtcgcatgg tggaaaagtc atccacaacc aaaaatttaa
     1561 gttctttatc cgccatttca cactcctgat ttaaatacgt atcgcctgtc cggcactaat
     1621 ttttgccaac atttgctggc ttacctggct aagatcgacc acttcgcaga caccacccat
     1681 attgatggcc tcgcgcggca tgccgaacac cacgcaactt gcttcgtttt gcgcaagggt
     1741 ccatgccccc gcctgacgca tcgccaacat tcccgccgcg ccgtcgttgc ccataccggt
     1801 caggatcacc ccaaccgcat tacgccccgc ctgtttggcg acagaatgga acaacacatc
     1861 taccgaaggc cgatgacggt taaccgccgg gccgtcgtga attttgattt gataatttgc
     1921 gccactacgc gccagttcca tatgccgatc gcccggcgca atataggcat gccccggcaa
     1981 gacacgttct ccgtcttcgg cttctttaac cccgatctgg caaagcttat taagtctgtc
     2041 ggcaaaagag cgggtgaaac cgggcggcat atgttgggta attaacaatg ccgggctgga
     2101 aagcggcaac ggttgcagta cgtgacgaat tgcctcagtt ccacccgttg aagcaccaat
     2161 cgcaatcagt ttttcagaac tcaacaacgg acccgccttc agcgttgtcg gtgctgacaa
     2221 tggcttatgt gctgcaaggc tcgcctttgc tgccgtacgc accttttcag caatcatttc
     2281 gctatacgcc agcattcctt cgcgaatacc cagttgcggt ttggtgacaa aatctattgc
     2341 ccccagctcc agcgcgcgca gcgtgacttc tgatcctttg ccggtcaagg aagaaaccat
     2401 cacaacgggc attggacgca aacgcattaa tttttcgagg aaatccagtc cgtccatccg
     2461 cggcatttca acatccagcg tcagcacatc gggattgaat ttcttaatca agtcacgcgc
     2521 gaccagcgga tcaggcgcgg tcgccaccat ttccatgtcg ctatggctgt tgatgatttc
     2581 tgtcatgatc tggcgcatca gtgccgaatc atcgacagat aacaccctga ttttgctcat
     2641 cgttaatcct tacttagcgc atacaccgtc tgaccacgca gcgtgaagcg gcgctcaagg
     2701 tggctaaagt tttcagagta acccgcaaac agcaatccgt cgggtttaag gagcggaaca
     2761 aagcggcgca aaatctcctg ctgggtattt tgatcgaagt agatcatgac gttacgacag
     2821 aagatcgcat caaacggccc cggcacggtg tactgtttcg ccagtagatt cagcggggca
     2881 aaatcaacat agttcgccag ctcctgacgc acgcgtacca gcccttcatg cggtcccgtc
     2941 cctcgcatga aataccgttg cagttgctgc ggcgtcaggt ttttcaactc ttcatggcga
     3001 taaataccgc ttctggcttt ttccagcact tcggtgtcga tatcactggc aaacactttc
     3061 cagcgtccgg gcgcggtgcc caatgtgtca gccagcgtca tcgcaatgct gtacggctct
     3121 tcgccggtcg aagccgccgc gctccatacg cgatactcgc cagaacggcg atgtgcgtga
     3181 tccgcgagca gagggaaatg gtgtgcctca cggaaaaatg ccgtcagatt cgtggtcagc
     3241 gaattgataa acgcctgcca ctcaccgctg tgctgattag attccagcaa gttcagataa
     3301 tgaccgaaat ccgtcagtcc cagcgaacgc aaacgacgaa ccagtcggtt gtaaaccatg
     3361 tcgcgtttat ggtcagccag aacgatcccg gctcgttgat agatcaattg acttatccgc
     3421 cgaaaatgcg cgtcggaaag cgccaggcgc tcggtcatct gtaacaataa agacgtttgc
     3481 ccacagggca gagatgaagt catagcgcct tctcaatcac ttcaggatac cactggcgca
     3541 atttgtaact gcgccgactc atgtcgtgct acttcatgtt cttcaagggt aaataccgcc
     3601 acacgcgacg aaagatggtc ggcctgattc gccagttgtt ccgttgccac cgccgcttct
     3661 tctaccagcg aggcgttctg ctgagtcacc tgatccatct ggctgacagc ctgtgcaacc
     3721 tgctcaatcc cccgctgttg ttcttccgac gcagaggcaa tttctcccat aatgtcgttc
     3781 acgcgggtca ccgaactgac aatatcgatc atggtcgcgg cggcgttatt caccagtttc
     3841 gaaccctgct ggacacgatt gactgactct tcgatcagcc ctttgatctc ttttgctgcc
     3901 tgcgcgctac ggctggcaag attgcgcact tcacctgcca ctaccgcaaa accacgcccc
     3961 tgctctccgg cgcgagccgc ttccactgcc gcattcaggg ccagaatatt ggtctggaaa
     4021 gcaattccgt cgataacgct gataatgtcg ccaatttttt gcgagctggt ggcgatctcc
     4081 tgcatggtgt gagtcatggt actgacctga acaccgcccg cctgcgccgt tgtcgcggca
     4141 ttttttgcca gttccgacgc ctgtcgtgcg ttatcggcgt tttgccctac cgtggcggtt
     4201 aattgctcca tactggcggc cgtttgtgcc agcgatgccg cctgctgttc ggtacgactt
     4261 gagagatcgt tattgcctgc gacaatctcc gcgataccaa tgtgcatctc ctggcttccc
     4321 ttacgcacat cacttaccgt cccacgcaaa gcctgctgca tggtcttcag actggcaaaa
     4381 atggcggtga tctcattacg accatatacc gcaatcggac gcgccagatt acccgcagca
     4441 atgctgtcaa aatggctacc gataatggcc agtggttgaa caatcatctt gcgcgtccac
     4501 cacagcgcac tgctgatgta gatcgccgca acaataatca tgctgataaa caccagtgtc
     4561 gaaatctgat agttacgctg gctttgcgca ctggcggctt ccagcacatg gttgatctcc
     4621 agctgccagg cctcaaagtt aacgtcaaac gcattctgtg atccctgcac cggcgcagtg
     4681 aggaaatccg aaagttgatt actttcaagc caggtcgcct gatgttcgag atcgttgtgc
     4741 cagcgggcaa aacttttctc cgtctctttt tgcaatgccc tgacgtgctc gttgcccgca
     4801 gtcatcgcca taaaactttt aaacagcgtg gtggattgcg tcagactggc gcgcgccgtc
     4861 gtcatcaacg ttttaatgtc atccgccgga tagctaagtg ccgtcagagt gcccgctttg
     4921 ttcagcgcgg tactggcctg taacattacc gcccgagttt gcgctaatgc cgcacgttgc
     4981 tgattgctct gctcgacctg attcagtcgt tgcaaatcgt cgcgaaatgc ccaaaaagac
     5041 atgccgttac tgccaatctg caagatcccg cagagaatca aaattaaaaa cagcgtggtc
     5101 gaaattcgaa tacgattaaa catcaacgct ccccatcagg cggcaatgac cgcgttagta
     5161 aatactcgtc aaaatgtttc ccagtttgga tcttgttcag caattcgcag tcgtggctgt
     5221 gccggtggtt gctcactggc aggacgggat ggtgtttgcg gtttattggt gagtgggctg
     5281 gctgccagac ggaacgcgga aaccgcttgc gttaaacgac tcgcctgttc ttccagcgca
     5341 gcggcggcgg cagctgattc ctgcaccagc gatgcgttct gttgcgtgac gcgatccatt
     5401 tccgaaaccg ccaatgcgac ttgatcgatg ccacggctct gttcatccga tgccgatgca
     5461 atctcgccca taatgtcagt cacgcgagtg acagcattga cgatattgtt cattgtttcc
     5521 ccggcgcttt cgaccagcac cgaaccggta tcaacgcgtg cgacggagtc ttcaatgagg
     5581 gctttgatct cttttgccgc ctgggcgctg cgactggcaa gattacgcac ttcacccgcc
     5641 accacggcaa aaccacggcc ctgttcaccc gcacgcgcgg cttcaaccgc ggcattcagc
     5701 gcgaggatat tagtctggaa ggcaataccg tcgataacgc tgataatgtc ggcaattttc
     5761 ttcgaactat cggcgatctc atgcatcgtt ttcactacgc catccaccac tttgccgccg
     5821 tgctgggcgg tgtcggaggc actttgcgcc agttgcgagg cctggcgggc gttatcggcg
     5881 ttttgcttca ctgtcgcggt gagctgctcc atgctggcgg cagtttcttc cagcgcggat
     5941 gcctgctgtt cagtacggga ggaaagatcg gtgttgcccg ccgcaatttc acgggtaccg
     6001 gcatagatgg catctgaacc ttcgcggaca tgagtgacgg tgtcagtcaa agagcgttgc
     6061 atatgtgaaa cgctctgcgc caggtcgccc atttcactgc gcccgtcaat ggtcagggta
     6121 ttcgctaggt taccaccggc gatttcgcga atgtgagcaa taatttttgc cagcggagta
     6181 agcaacatac ggcgaatgcc gtaccacgcc accagcagaa tcaataccac caccagcgcg
     6241 ataaccgcca gttgccactg ggcaaatcgg taatcatctg cgttgtcagt gacgatatcg
     6301 cgatacagtt tttcactgct gagggcgtac tgagcaaacg cttcgcccat tgcattttgc
     6361 attccctggg ttggctgagc gaaataagct ccagtattgc cataatctag ataatcaatc
     6421 agttcagtta acgctgtgta atagttttta tatttttcat caatattacg actggtagcg
     6481 accatttcag gtaacggtgc catgcttttg aattttttat aatgcgtcgc tgcctgcgcc
     6541 aatgttttcc tggcgctatc gagcaattca actttggcgt tactttgttg attggaggaa
     6601 tccatcatca tccgcaccgc tgaacgactc aggttaatgc gcgtttgcag cattaaatcc
     6661 caggttgacg tcagctcgcc ctgctgttcc cgcaattgat tggaaaccac aaagctcttc
     6721 tggctatggt gaagggaaga aaaaaacaga ctgccggaaa taagctgtaa cagtgcgaat
     6781 acccccagca ccattaccaa cagcgtgact acgcggatac ggttaatcat aaggcacctt
     6841 cctgaaaaca agttgatctc gttatcggca agaagggggg aaactttatt gctgatgcca
     6901 cccgccgcga aattgaaata aaaaacccga tgcgcagatc atcgggttca tttcaattga
     6961 ggaaatcggg agaattacgc cacttctgac gccgcgctat ctaacagcgc catctcttcg
     7021 ctgttcagca gtttttcgat gttcaccaga atcaacatac ggtcgcccag tgcgcccagt
     7081 ccagtgagat attctgttga aagcgtcacg gcaaactctg gtgccggacg aatttgctcc
     7141 gccgtcaatg aaagcacgtc tgaaacgccg tcaaccacga tgccgaccac ccgctgtccg
     7201 agattcagga cgataactac cgtgttgtcg ttatagtcca catccacctg gctgaactta
     7261 attcgtaagt caacaatcgg cacaataacg ccgcgcagat tcgtgacgcc tttgataaac
     7321 gctggcgtgt tcgcaatccg tgttacctga tcgtagccac ggatctcctg cactttcagg
     7381 atatcaatac cgtactcttc atcaccaagg gtaaatacca gaaattcctg gcctgacggc
     7441 tcgctggcca gctttgttac attcgtcata ccggtcatat tgttaccttt ttactcattc
     7501 aggcggcggt gttcgccata cgttgttcgc ggtttatcgc ctgcaaggcg gagacatcaa
     7561 caatcagtgc cacgctgccg tcgccaagaa tggtcgcagc agaaatgccg gggactttgc
     7621 gatagttact ttcaaggttt ttaaccacaa cctggtgttg accaattaat tgatccacca
     7681 gcaaggcgta gcggcgaccg ccactttgta agatcacaac aattccctgg gtggcttcgg
     7741 ttttcgcgcc cgcgacgttg aacactttcc acagttcgac gatgggcaga tattcacccc
     7801 gcacttccag tacccgctcg ccgccggcca gtggatggag atcggcttca cggggttgca
     7861 gtgattccat aacagcattc agcggcagaa tgaaaacttc atccccaacg cgtacggaca
     7921 tgccgtcgag gatggccagc gtcagcggca gtaaaatgcg gatcgtagtg ccagtcccct
     7981 gcttcgactg gatttcgaca tgaccgccca tctcctggat attacgttta acgacgtcca
     8041 tgccgacgcc gcgcccggag acgtcggtga cctgctctgc catggagaag cctggtgcaa
     8101 atatcagcat cgcgacttcg tcgtcgctca tgttttcgct gacagtcaaa ccttgcgagg
     8161 ccgcttttgc cagaattcgc tcacggttta gccccgcccc atcgtcggtc acttcaatgc
     8221 aaatgttgcc gccctgatgt tcggcagaca gaattaaatt tccgacgctg tttttacctg
     8281 cggcgagccg tttttctggc agttcaatac cgtgatcgag gctattgcgt accaggtggg
     8341 tcagcgggtc gataatgcgt tctatcaggc ttttgtcgag ttcagtagaa ctgcccacca
     8401 gcgtcagttc tacctgcttg ccgagttttc ccgccagatc acgcaccagc cggggatagc
     8461 gactaaaaac atattccatc ggcatcatgc gaatcgacat cactgattcc tgcaaatcac
     8521 gggcgttacg ttgtaactgc cccatgctgg ttatcaaatc accatgatta accgggtcca
     8581 gttcgctgga acgctgggca agcatggact gggtgataac cagctcgccg acgaggttaa
     8641 ttaattgatc aaccttttct accgctacac ggatgctggt ggattcattg ctgcgcgtcg
     8701 ttttttcccg ctccacgcgg ccggttggcg cttgttcggc tgccagttta agcactggtg
     8761 gggtggatat ttttggcgag acttctactg tttcaaaggt aatctgatcg gcttcaatca
     8821 caaaacagag taccgctgtg atgtcatctt cggcgatgtc gcccggtaat attgccgaga
     8881 gcgaatccgc ccctttcacc acgtcagtta acgttgtcag atgtcccagt tcttcttcca
     8941 gcaggtcgac ttccccggcc ttcaggcgcg aaaggataat tcgtcgcggc gactgactgc
     9001 gactctgctc atcttgcggt tcacttttgg caaccacact taatcgggtc actgcggatg
     9061 gcgtttcgcc tttcgcttct aatgccagtt gacgcaaggc ctggcagata taatcgaagc
     9121 tggcggcatc cggctcttgc gactgtttat aagcgtcgag ctgttcttgc atgatgtcct
     9181 tcgtttccaa aaacagattg ataatgtcgg tgttgagttg catctcacct cgtctggctt
     9241 catcgagcag gttttccatc agatgcgtgg tttcctgcaa aacgctgaag ccaaaagttc
     9301 ctgcccctcc tttgatcgag tgggcagccc gaaagatggc attcaattgt tcggcatctg
     9361 gcgcttccgg ctgcaaaacc agcagatgct gctccatgtc agccaacagt tcgtccgctt
     9421 catcaaaaaa tgtctgataa aaatcgctta tatccatgct cacgctgtca cctcggttcg
     9481 gctgatggca ttgtgggaac actgacctgt ggtgcaacct caggtttttc cagggcgctt
     9541 actggctcat tctggctttc ggcgttttca tgcaaaatgg cctgttcggc ttgtttgttc
     9601 agtaccagca ggctgatgcg acggttgacg gcatcatcag gtccgcgatc gcttaagcgc
     9661 atcgttgccg ccatgccgac gacacgtaac actttgccgc tatccaaccc tccgaccatc
     9721 agttcgcggc gggatgcatt ggcccgatcg gcagaaagct cccagttgct atatcctttc
     9781 tcaccgctgg cgtaggggaa atcatcggta tgacctgaaa ggctaatacg gttgggaata
     9841 ccgttcagta caggcgcaat gacacgcaga atgtcgcgca tatagggttc gacatcggca
     9901 ctgccggttc taaacatcgg gcgattctgg ctatcgatga tctgaatacg tagaccttcc
     9961 tggaccagat cgattttgag atggggacgt aacgcccgca gtttcggatc ggactctatc
    10021 aactggtcga gatcaccccg caatttccgc aatcgacttt gctccatgcg ttttttcaga
    10081 tcttcgatgt tcggctgctt attcacttcc ccctggcttt gggtgtaatc atcaccaccg
    10141 ccgggaattg ggctttcact attagaaatg cgatcgccgc ccgtaaccgc agtcgccagt
    10201 ggagtccgga agtactccgc aatctgaatc agctcttttg ggctggagat ggagatcagc
    10261 cacatcacca gaaaaaaggc catcatcgca gtcataaagt cggcataagc aatcttccac
    10321 gatccatgtg ctgccccgtg gcttttggct ttgcgtcgtt tgacgacaat aatcggatgc
    10381 gcttgattct tcatgcttcc tcggttgtcg tctgttgttg cggatttttc accgcacgca
    10441 catgctcttc cagttcaata aacgacggac gttcgctgga atagagcgtt ttgcgaccaa
    10501 actcaacggc gataggcggt gcgtaaccgt tcagattaga aagcagagtg actttgacgc
    10561 actgcatcat tttgctggtt tcggcgcttt tctgacgtaa aacactcgct aatggggaaa
    10621 taaatccgta agccaataaa atgccgagga aagtccccac catcgcatgt gcgataagcg
    10681 cacccagctc ggcggcagga cgatcggctg aacctaacgc gtgaacgccc cccattacag
    10741 ccgcgacaat accaaacgcc ggaagtgagt ccccgaccag cgccagactg ttcgccggga
    10801 cttctgcctc gctttcgtgc gtctcaatct cttcatccat cagggcttcg atttcgaagg
    10861 tgttcatatg accgctgata atcagacgca gataatcgac gataaaatca agcatgacgc
    10921 tatccgcgag gatgcgtggg tagctggcga agatctcgct ctcacgggga ttttcaatat
    10981 cacgttccag cgaaaacatc cccatctgcc gcgatttcgc catcaaccga taaagcagag
    11041 ccagcagatc catatacatt gctttggtgt atttggagcg acgaaacagc aacggcagcg
    11101 ccttcagcgt gcctttaatc gctttgccat tattgccgac gataaacgac ccaatccctg
    11161 caccggcaat aatcaccagt tcagcgggtt gatagagtgc tccaaggctt ccaccggtca
    11221 tcaaataacc gccgaaaact gtaccgagaa caaccaggta acctaataag ataagcacga
    11281 catcatcctt ccactgttga ccatgacagg atgttcagtc gtcaggcgtt aacgcgcgat
    11341 tggggcaaaa aaagcagcgg tacgtcgtta ccgctactgg aatgttgcgc ctcaccgtat
    11401 cagttaaaca gcctgtactc tctgttcatc cagcagttgt gggataatat cggcaggatt
    11461 ctgggaaagt ttacgtcttt ttactgcccg ggatggcggt tgacataagc tgcaggcaaa
    11521 gctgccaaca ggctggtgag cgtgggtaat aaaattgccg ccgcagcagt tgcagctgga
    11581 aagttgcagt aatccacttt caacaaaccg caccaatgtc caggcacggg ttaatgccag
    11641 cagtggtcct tcttctgctt gtgggcactg ttcaaggtat aaacggtagg ctttgatcac
    11701 cgcatcgacg ccattacaca aaccggtttt cagtaaaaac tgccatgcat tacagaacat
    11761 cgaagcatga acgttttgtt cccaggtcat aaaccagtcg gttgagaatg gcagcatgcc
    11821 tttcggcggt gggcttccgc gcagttcttt ataaagtttt atcaggcgtc cgcgacttaa
    11881 ctgtgtttcg ctttccagca tctgcaaacg agcgcccagg gtaatcaatt ccattgccag
    11941 ctgaatatcc cgcgcttcct gaacaatgct tttttcactc atgatcaggc ccttttcttg
    12001 cgcagcgctt cttcaggctg attaacatca ttcagcaagc gtgttgagag catgatgccg
    12061 gtatgaattt gctggagatc gtcaacgcgg gaatcttgcg tcaactgagt aatcgtctgg
    12121 tggctgtcaa aacggaagtg acaaaccagt tgattggttt ctgccagctt aaccatttgc
    12181 ggaagagtca gtgccgctaa cgttgtcgcc atttcttcat ttatgccgag acgaaacata
    12241 gcggacgctt tgtcctgaac aatcaaacgc tgtgcaagta gtaaatatga caagttgatg
    12301 tcataaatgt gtttcagcaa ctcggaggta tgcattattc ccacccagaa taaccaact
//
LOCUS       DADMKF010000117        12245 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.119, whole genome shotgun sequence.
ACCESSION   DADMKF010000117 DADMKF010000000
VERSION     DADMKF010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12245
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.119"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            <1..1224
                     /locus_tag="JNC24_003000"
     CDS             <1..1224
                     /locus_tag="JNC24_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY9159692.1"
                     /translation="VTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGG
                     RRWTVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFP
                     KAKQGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAW
                     IWVPLLAIATIAAWSGMNDIASSRASIDDQLPVLQRLHLWLLSLLYLATFGSFIGFSA
                     GFAMLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSA
                     LLFLTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSD
                     EQAQKEAITETAAALGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCV
                     LLTWLVYGRRKFSQK"
     gene            1306..5046
                     /locus_tag="JNC24_003001"
     CDS             1306..5046
                     /locus_tag="JNC24_003001"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HAY9159693.1"
                     /translation="MSKLLDRFRYFKQKGETFADGHGQVMHSNRDWEDSYRQRWQFDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWEIQQTDYPRTRPDQPNHEPRGCPRGASYSWY
                     LYSANRLKYPLIRKRLIELWREALKQHSDPVLAWASIMNDPQKCLSYKQVRGRGGFIR
                     SNWQELNQLIAAANVWTIKIYGPDRVAGFSPIPAMSMVSYAAGTRYLSLLGGTCLSFY
                     DWYCDLPPASPMTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTIAITPDYSEVAKLCDQWLAPKQGTDSALAMAMGHVILKEFHLDNPSDYFINYCRRY
                     SDMPMLVMLEPRDDGSYVPGRMIRASDLVDGLGESNNPQWKTVAVNTAGELVVPNGSI
                     GFRWGEKGKWNLESIAAGKETELSLTLLGQHDAVAGVAFPYFGGIENPHFRSVKHNPV
                     LVRQLPVKNLTLVDGNTCPVVSVYDLVLANYGLDRGLEDENSAKDYAEIKPYTPAWGE
                     QITGVPRQYIETIAREFADTAHKTHGRSMIILGAGVNHWYHMDMNYRGMINMLIFCGC
                     VGQSGGGWAHYVGQEKLRPQTGWLPLAFALDWNRPPRQMNSTSFFYNHSSQWRYEKVS
                     AQELLSPLADASKYSGHLIDFNVRAERMGWLPSAPQLGRNPLGIKAEADKAGLSPTEF
                     TAQALKSGDLRMACEQPDSGSNHPRNLFVWRSNLLGSSGKGHEYMQKYLLGTESGIQG
                     EELGASDGIKPEEVEWQTAAIEGKLDLLVTLDFRMSSTCLFSDIVLPTATWYEKDDMN
                     TSDMHPFIHPLSAAVDPAWESRSDWEIYKGIAKAFSQVCVGHLGKETDVVLQPLLHDS
                     PAELSQPCEVLDWRKGECDLIPGKTAPNIVAVERDYPATYERFTSLGPLMDKLGNGGK
                     GISWNTQDEIDFLGKLNYTKRDGPAQGRPLIDTAIDASEVILALAPETNGHVAVKAWQ
                     ALGEITGREHTHLALHKEDEKIRFRDIQAQPRKIISSPTWSGLESDHVSYNAGYTNVH
                     ELIPWRTLSGRQQLYQDHPWMRAFGESLVAYRPPIDTRSVSEMRQIPPNGFPEKALNF
                     LTPHQKWGIHSTYSENLLMLTLSRGGPIVWISETDARELTIVDNDWVEVFNANGALTA
                     RAVVSQRVPPGMTMMYHAQERIMNIPGSEVTGMRGGIHNSVTRVCPKPTHMIGGYAQL
                     AWGFNYYGTVGSNRDEFIMIRKMKNVNWLDDEGRDQVQEAKK"
     gene            5043..6587
                     /gene="narH"
                     /locus_tag="JNC24_003002"
     CDS             5043..6587
                     /gene="narH"
                     /locus_tag="JNC24_003002"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HAY9159694.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQIDDYYE
                     PFTFDYEHLHSAPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNF
                     EAMQKEMYGQFKNTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRG
                     WRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDA
                     DRIEEAASTEREVDLYERQCEVFLDPHDPSVIEEALKQGIPQNVIEAAQRSPVYKMAM
                     DWKLALPLHPEYRTLPMVWYVPPLSPIQSYADAGGLPKSEGVLPAIESLRIPVQYLAN
                     MLSAGDTGPVLRALKRMMAMRHYMRSQTVEGVTDTRAIDEVGLSVAQVEEMYRYLAIA
                     NYEDRFVIPTSHREMAGDAFAERNGCGFTFGDGCHGSDSKFNLFNSSRIDAINITEVR
                     DKAEGE"
     gene            6587..7282
                     /gene="narW"
                     /locus_tag="JNC24_003003"
     CDS             6587..7282
                     /gene="narW"
                     /locus_tag="JNC24_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HAY9159695.1"
                     /translation="MQILKVIGLLMEYPDELLWECKEDALALIRRDAPMLTDFTHNLL
                     NAPLLDKQAEWCEVFDRGRTTSLLLFEHVHAESRDRGQAMVDLLAEYEKVGLQLDCRE
                     LPDYLPLYLEYLSVLPDDQAKEGLLNVAPILALLGGRLKQREAPWYALFDALLQLAGS
                     ILSSDSVTKQVNSEERDDTRQALDAVWEEEQVKFIEDNATACDSSPLNQYQRRFSQDV
                     APQYVDISAGGGK"
     gene            7279..7959
                     /gene="narI"
                     /locus_tag="JNC24_003004"
     CDS             7279..7959
                     /gene="narI"
                     /locus_tag="JNC24_003004"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HAY9159696.1"
                     /translation="MIQYLNVFFYDIYPYICATVFFLGSWLRYDYGQYTWRASSSQML
                     DKRGMVIWSNLFHIGILGIFFGHLFGMLTPHWMYAWFLPVAAKQLMAMVLGGICGVLT
                     LIGGAGLLWRSLTNQRVRATSTTPDIIIMSILLIQCLLGLSTIPFSAQYPDGSEMMKL
                     VGWAQSIVTFRGGSSEMLNGVAFVFRLHLVLGMTIFLLFPFTRLVHVWSAPFEYFTRR
                     YQIVRSRR"
     gene            8038..8931
                     /locus_tag="JNC24_003005"
     CDS             8038..8931
                     /locus_tag="JNC24_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
                     /protein_id="HAY9159697.1"
                     /translation="MKPQVYHVDAFTSQPFRGNSAGVVFPADNLSEAQMQLIARELGH
                     SETAFLLHSDDSDVRIRYFTPTVEVPICGHATVAAHYVRAKVLGLGNCTIWQTSLAGK
                     HRVTIEKHNDDYRISLEQGTPGFELPLEGEVRAAIINALHLTEDDILPGLPIQVATTG
                     HSKVMIPLKPEVDIDALSPDLNALTAISKQIGCNGFFPFQIRPGKNETDGRMFSPAIG
                     IVEDPVTGNANGPMGAWLVHHNVLPHDGNVLCVKGHQGRALGRDGMIEVTVTIRDNQP
                     EKVTISGTAVILFHAEWAIEI"
     gene            complement(9027..9872)
                     /gene="nhoA"
                     /locus_tag="JNC24_003006"
     CDS             complement(9027..9872)
                     /gene="nhoA"
                     /locus_tag="JNC24_003006"
                     /EC_number="2.3.1.118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-hydroxyarylamine O-acetyltransferase"
                     /protein_id="HAY9159698.1"
                     /translation="MTPILNHYFARINWSGAAAVNIDTLRALHLKHNCTIPFENLDVL
                     LPREIQLDDQSLEEKLVIARRGGYCFEQNGVFERVLRELGFNVRSLLGRVVLSNPPAL
                     PPRTHRLLLVELEGEKWIADVGFGGQTLTAPIRLVSDLVQTTPHGEYRLLQEGDDWVL
                     QFNHHQHWQPMYRFDLCEQQQSDYVMGNFWSAHWPQSHFRHHLLMCRHLPDGGKLTLT
                     NFHFTHYENGHAVEQRNLPDVASLYAVMQEQFGLGVDDAKHGFTVDELALVMAAFDTH
                     PEAGK"
     gene            10045..10614
                     /locus_tag="JNC24_003007"
     CDS             10045..10614
                     /locus_tag="JNC24_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HAY9159699.1"
                     /translation="MSRFIPIELHHASRLLNHGPTVLITSFDEQSQRRNIMAAAWSMP
                     VEFEPPRVAIVVDKSTWTRELIERNGKFGIVIPGVAATNWTWAVGSVSVREEDKFNCY
                     GIPVVKGPVLGLPLVEEKCLAWMECRLLPATSAQQKYDTLFGEVVSAAADARVFVEGR
                     WQFDDDKLNTLHHLGAGTFVTSGKRVTAG"
     gene            complement(10618..10845)
                     /gene="pptA"
                     /locus_tag="JNC24_003008"
     CDS             complement(10618..10845)
                     /gene="pptA"
                     /locus_tag="JNC24_003008"
                     /EC_number="5.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase PptA"
                     /protein_id="HAY9159700.1"
                     /translation="MPHIDIKCFPRELDEQQKAALAADITDVIIRHLNSKDSSISIAL
                     QQIQPESWQAIWDAEIEPQMEALIKKPGYSM"
     gene            complement(10945..12080)
                     /locus_tag="JNC24_003009"
                     /pseudo
     CDS             complement(10945..12080)
                     /locus_tag="JNC24_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
BASE COUNT         2896 a         3149 c         3165 g         3035 t
ORIGIN      
        1 gttaccgtta atctcaataa aatcggtttt aattttacta ccgatcaact ctttttatta
       61 accgcattac cctccgtttc tggcgcatta ttgcgtgttc cctactcctt tatggtgcct
      121 atattcggtg gacgccgatg gacggttttt agtactgcaa tcctgattat tccttgcgtc
      181 tggctcggaa ttgccgtgca aaatccgaat actccttttg ggatatttat cgttatcgct
      241 ttgctatgcg gttttgcagg tgcaaacttt gcttcgagca tgggcaatat cagtttcttc
      301 tttccaaaag ccaaacaagg gagcgctctt gggattaatg gcggattagg aaacttaggt
      361 gtaagtgtaa tgcagctggt tgcaccgctg gtcatttttg tacctgtatt tgcctttctc
      421 ggcgtcaatg gcgtaccgca ggccgacggt tcggtgatgt cgctggcgaa tgccgcatgg
      481 atttgggtac cgctactggc gattgccacg atcgccgcct ggtcagggat gaatgatatc
      541 gccagttcac gcgcctcaat tgacgaccag ctccctgtct tacaacgcct gcatctctgg
      601 ctgctgagcc tgctttacct tgccaccttc ggttcgttta tcggtttttc tgcgggtttt
      661 gccatgctgg caaaaaccca gttcccggat gtgaatattc tgcgcctggc gttctttggc
      721 ccatttatcg gtgccatcgc gcggtcggtt ggtggtgcta tttccgataa attcggcggc
      781 gtgcgggtga cgttgatcaa ctttattttt atggcgattt tcagtgccct gctgttcctt
      841 accttaccgg gcacaggctc cggtaatttc atcgcctttt acgccgtatt tatggggctg
      901 tttctgactg cgggtctggg aagtggttct actttccaga tgatcgccgt catctttcgc
      961 cagataacca tttatcgggt gaagatgaaa ggcggtagtg atgagcaagc tcagaaagaa
     1021 gccatcaccg aaacggcggc ggctctgggc tttatctcag ccattggcgc agtgggcggc
     1081 ttttttattc cgcaggcgtt tggcatgtcg ctcaatatga ccggctctcc ggtcggcgcg
     1141 atgaaagtgt ttttaatctt ctacatcgtt tgtgtgctgc tgacctggct ggtttatggt
     1201 cggcggaagt tcagccaaaa ataagagatc gatgcctgat gtacaaccgt gtcgcatccg
     1261 acaaggcatc agatcccaat tcattcctgg agcaggagtc atgtcatgag taaacttttg
     1321 gatcgctttc gctacttcaa acaaaagggc gaaacctttg ccgatggtca cggacaggtg
     1381 atgcatagca accgcgactg ggaggacagc tatcgccagc gttggcagtt cgacaaaatt
     1441 gtgcgttcca cccacggtgt taactgtaca ggctcctgta gctggaaaat ctacgttaaa
     1501 aatggtctgg tgacctggga aatccaacag accgactacc cgcgcactcg ccctgaccag
     1561 cccaatcatg aacctcgcgg ctgcccgcgt ggcgcaagtt actcctggta tctctacagc
     1621 gctaaccgcc tgaaataccc gctcattcgt aaacgactga ttgaactgtg gcgtgaagct
     1681 ctcaagcaac acagcgatcc ggtactggcg tgggcatcga ttatgaacga tccgcaaaag
     1741 tgcttaagct acaaacaagt gcgtgggcgc ggcgggttta tccgctccaa ctggcaggaa
     1801 cttaaccaac tgattgccgc cgctaacgtc tggaccatca aaatctatgg tccggatcgc
     1861 gttgccggtt tctcgccgat cccggcgatg tcgatggtgt catacgctgc cggaacgcgt
     1921 tatctgtcgc tgttaggcgg cacctgttta agcttctacg actggtattg cgacctgccg
     1981 cccgcctcgc cgatgacctg gggcgaacaa accgacgtgc cggaatctgc cgactggtat
     2041 aactccagtt acatcatcgc ctgggggtct aacgtaccgc agacacgtac gccggacgcc
     2101 cacttcttta ccgaagtacg ttacaaaggc accaaaacta tcgccattac ccctgactac
     2161 tctgaagtgg ccaaattgtg cgaccagtgg ctggcaccga aacaaggcac tgatagcgcc
     2221 ctggcgatgg caatgggcca tgtgatttta aaagagtttc atctcgataa tcccagcgac
     2281 tactttatca actactgccg ccgctatagc gacatgccga tgctggtaat gctggaacct
     2341 cgcgacgatg gtagctacgt acccgggcgc atgatccgcg cgtctgacct ggtagatgga
     2401 ctaggcgaaa gcaataatcc gcagtggaaa accgtggcgg ttaataccgc aggtgaattg
     2461 gtagtgccga atggttcgat tggtttccgc tggggagaaa aaggcaaatg gaatctggaa
     2521 tccattgccg ccggtaagga aaccgaattg tcgttaaccc tgctcggtca acatgacgct
     2581 gttgcaggcg tggccttccc ctactttggc ggcattgaaa atccgcattt tcgcagcgta
     2641 aaacacaatc cggtgctggt gcgccaattg cccgttaaaa acctgacgtt agtcgatggc
     2701 aacacctgtc cggtggtcag cgtttatgat ttggtgctgg cgaattacgg cctcgatcgc
     2761 gggctggaag atgaaaacag cgcgaaagat tacgctgaaa tcaaaccgta caccccagcc
     2821 tggggtgagc aaattaccgg cgtaccgcgc cagtatattg aaaccatcgc ccgcgaattt
     2881 gccgataccg cccataaaac gcatggacgc tcaatgatta tcctcggcgc tggcgtcaac
     2941 cactggtatc acatggatat gaactaccgt gggatgatca atatgctgat cttctgcggt
     3001 tgtgtcgggc aaagcggcgg cggctgggcg cactatgtcg gtcaggaaaa actgcgccca
     3061 caaaccggct ggttgccgct ggcctttgcg ctcgactgga accgcccacc gcgccagatg
     3121 aacagtacct cgtttttcta caatcattcc agccaatggc gctatgaaaa agtctctgct
     3181 caggagttac tttcaccgct tgccgatgcc agtaagtaca gcggtcatct gattgatttc
     3241 aacgttcgcg ccgaacgcat gggctggcta ccttctgcgc cgcagctggg gcgtaacccg
     3301 ctcgggatta aagctgaagc cgacaaggcc ggattatccc ccacagaatt taccgcccag
     3361 gcgctgaaat cgggcgattt acgtatggcc tgcgaacaac cagatagcgg cagcaatcat
     3421 ccgcgtaatt tgtttgtctg gcgctctaac ctgcttggct cctccggcaa aggccacgag
     3481 tatatgcaga agtatctgct ggggaccgaa agcgggattc agggcgagga actcggtgcc
     3541 agcgacggga tcaaaccgga agaagtcgag tggcaaactg cggcgattga aggcaagctc
     3601 gacctgctgg tgacgctcga cttccgcatg tccagtacct gcctgttctc cgatatcgtt
     3661 ctgcccaccg ccacctggta cgaaaaagac gatatgaaca cctcggatat gcatccgttt
     3721 attcatccgc tttctgcggc ggtcgatccg gcgtgggaat cacgcagcga ctgggaaatc
     3781 tacaaaggta tcgccaaagc attttcgcaa gtgtgcgtgg gtcatcttgg caaagaaacc
     3841 gacgtggtat tacaacccct actgcatgac tctccggcag agctctcaca gccgtgtgaa
     3901 gtgctcgact ggcgcaaagg cgaatgcgat ctgattccag gcaaaaccgc gccgaatatt
     3961 gtggcggtgg agcgcgacta ccctgctacg tatgaacgct ttacctcgct cgggccattg
     4021 atggacaaac ttggcaatgg tggtaaaggg atttcgtgga atacccagga tgaaatcgat
     4081 ttcctcggca aactcaatta caccaagcgt gatggcccgg cacaggggcg tccgctgatt
     4141 gacaccgcca ttgacgcttc agaagtgatt ctggcactgg caccggaaac caacggtcat
     4201 gttgcagtca aagcgtggca ggcgctgggc gagatcaccg ggcgcgaaca tacccatctg
     4261 gcgctgcaca aagaggacga gaagattcgc tttcgcgata ttcaggcgca gccgcgtaaa
     4321 attatctcca gccccacatg gtccggtctg gaaagcgatc acgtctccta taatgcggga
     4381 tacaccaacg ttcatgagtt aattccgtgg cgcacgctgt cgggacgcca gcagctctat
     4441 caggatcatc cgtggatgcg tgcttttggt gaaagcctgg tggcttatcg cccgcctatc
     4501 gacacccgta gcgtcagtga gatgcgccag atcccgccaa acggcttccc ggaaaaagca
     4561 cttaacttcc tgacgccgca ccagaaatgg ggcattcact caacctacag tgaaaacctg
     4621 ctaatgctga cgctctctcg cggtggaccg attgtctgga tcagcgaaac cgatgcccga
     4681 gaactgacca ttgtcgataa cgactgggtg gaagtcttta acgccaacgg cgcgctgacg
     4741 gctcgtgcgg tggtcagcca acgtgtaccg ccgggtatga ccatgatgta tcacgcccag
     4801 gaacgcatta tgaatattcc tggttcggaa gtaactggca tgcgcggcgg cattcataac
     4861 tcggtcaccc gtgtttgccc gaaaccgacg catatgattg gcggttacgc gcagctggcc
     4921 tggggcttta actactacgg caccgtcggc tcgaaccgcg acgaattcat catgatccgc
     4981 aagatgaaga acgttaactg gctggatgat gaaggtcgcg atcaggtaca ggaggcgaaa
     5041 aaatgaaaat acgttcgcaa gtcggcatgg tgcttaacct cgacaaatgt atcggttgcc
     5101 atacctgttc ggtgacctgt aaaaacgtct ggaccggacg cgaaggcatg gagtacgcat
     5161 ggtttaacaa cgtcgaaacc aaaccgggca ttggttatcc gaaaaactgg gaagatcagg
     5221 aagagtggca aggcggctgg gtccgcgatg tgaatggcaa gatacgcccg cgtctgggca
     5281 gcaagatggg cgtaataacc aaaatcttcg ccaacccggt ggtgccgcag attgatgatt
     5341 actacgaacc tttcaccttc gactacgaac atttgcatag cgcaccggaa ggcaaacata
     5401 tccctactgc tcgcccgcgt tcgctgattg acggtaagcg gatggacaaa gtgatctggg
     5461 ggccaaactg ggaagaactg ctgggcggcg agtttgaaaa acgtgcccgc gaccgcaact
     5521 tcgaggccat gcaaaaggag atgtacgggc agtttaaaaa caccttcatg atgtacctgc
     5581 cgcgcctgtg cgaacactgc ctcaatccca gttgcgtggc gacctgccca agcggcgcta
     5641 tctacaaacg cgaagaagac ggcattgtgc tgattgatca ggataaatgc cgtggctggc
     5701 gtttgtgcat tagcggttgt ccgtacaaaa aaatctactt caactggaaa agcggcaagt
     5761 cagaaaaatg tatcttctgt tatccgcgaa ttgagtcagg ccaacccacc gtgtgctcag
     5821 aaacctgcgt gggtcgcatc cggtatctgg gcgtgctgct ttacgacgcc gaccgcattg
     5881 aggaagcggc gagcaccgag cgcgaagttg acctctatga acgtcagtgc gaagtgttcc
     5941 tcgatccaca cgatccctca gtgatcgagg aagccctgaa acaaggtatt ccacaaaacg
     6001 tgattgaagc tgcccagcgt tcgcctgtct acaaaatggc gatggactgg aaactggcgc
     6061 taccgctgca ccctgaatat cgcaccctgc caatggtctg gtacgttcct ccgctgtcgc
     6121 cgattcagtc ctacgcagat gcgggcggtt tgccgaaaag cgaaggcgtg ctgcccgcca
     6181 tcgaaagcct gcgtattccg gtgcaatatc tcgccaatat gttgagtgcc ggcgataccg
     6241 gtccggtact gcgggcgctg aaacggatga tggcgatgcg ccactatatg cgttcacaaa
     6301 ccgtggaagg cgttactgat actcgtgcca tcgacgaagt aggcctgagc gtcgcccagg
     6361 tcgaagaaat gtatcgctat ctcgccattg ccaactatga agatcgtttt gttatcccga
     6421 ccagccatcg ggaaatggcg ggcgatgcct tcgcagaacg caacggctgc ggttttacct
     6481 ttggcgacgg ttgccacggt tcggacagta aattcaacct gttcaacagt agccgtatcg
     6541 atgccatcaa catcaccgaa gtgcgcgaca aagcggaggg cgaataatgc agatcctcaa
     6601 agtgatcggc ctgttgatgg agtatccgga tgagctgttg tgggaatgca aggaggacgc
     6661 gctggcgttg atccgccgcg acgcgccgat gcttacggat ttcacccaca acctgcttaa
     6721 cgcgccgctg ctggataaac aggccgaatg gtgcgaagtg tttgaccgcg ggcgcaccac
     6781 gtcgctgctg ctgttcgaac atgttcatgc cgagtcccgc gatcgcggcc aggcaatggt
     6841 ggacctgctg gcggagtatg aaaaggtcgg cctgcagctg gattgtcggg aactgcccga
     6901 ttatctaccg ctgtatctgg agtatttaag cgtactgcct gacgatcagg cgaaagaagg
     6961 attgctcaat gttgcgccga tcctcgccct gcttggcggt cgcttaaaac aacgcgaggc
     7021 accgtggtac gcgttgtttg atgctctgct gcaactggcg ggaagtatac tttcaagtga
     7081 cagtgtcaca aaacaagtaa acagcgaaga gcgcgatgac acccgccagg cgcttgatgc
     7141 agtgtgggaa gaggaacagg tgaagtttat tgaagataac gccacggcgt gtgacagctc
     7201 gccgcttaat caatatcagc gacgctttag ccaggatgtc gcgccgcagt atgtcgacat
     7261 cagtgcggga ggtgggaaat gattcagtat ctgaacgtct ttttttacga tatctacccg
     7321 tacatttgtg cgacggtgtt tttcctcggc agctggctgc gctacgacta cgggcagtac
     7381 acctggcgcg cctcctcaag tcaaatgctc gataaacgcg ggatggtgat atggtcgaat
     7441 ctgttccata tcggcatttt ggggattttc ttcgggcact tgttcggcat gttaacgccg
     7501 cactggatgt acgcgtggtt tttgccagtg gcagcgaaac aactcatggc gatggtgctc
     7561 ggcggtattt gcggtgtgtt gacgttgatt ggcggcgcag gactgctgtg gcgcagtctg
     7621 acaaatcagc gcgtgcgtgc cacttccact acgccggata tcatcatcat gagcattttg
     7681 ctgatccagt gtctgttggg gctaagtacc ataccgtttt cagcacagta tcctgatggt
     7741 agcgaaatga tgaagctggt gggttgggcg caaagcattg tcactttccg tggtggatcg
     7801 tcagagatgc tcaacggcgt agcgttcgtc ttccgcctgc atctggtgtt gggaatgacc
     7861 atcttcctgc tcttcccgtt cacccgactg gtgcatgtgt ggagcgcgcc gtttgagtac
     7921 tttactcgtc gatatcaaat tgtgcgttcg cggcgataat ttcaccctgg ccggatagcc
     7981 cttcgctctc cggccacttg caggctatga tagttctcca gtcacaggag aacgttaatg
     8041 aaaccgcagg tgtaccacgt cgatgctttt acctctcaac catttcgcgg caattctgcc
     8101 ggtgttgttt tccccgccga taatcttagc gaagcacaaa tgcagcttat cgcccgcgag
     8161 ttaggccatt cggaaactgc ttttctgctg cacagcgacg acagcgatgt gcgcatccgc
     8221 tactttacgc caacggttga agtgccgatt tgcggtcacg cgacagtagc tgcgcactat
     8281 gtacgtgcga aggtgttagg tttaggaaat tgcacgatct ggcaaacatc gctggcagga
     8341 aaacatcgcg tgactatcga aaagcacaac gatgattatc gtatttcgct ggaacaaggt
     8401 acgccgggct ttgaactgcc gctggagggt gaagtacgcg cggcgattat caacgcattg
     8461 catcttactg aagacgatat tctgccgggt ttgccgatcc aggtcgcgac gaccgggcat
     8521 tctaaagtga tgatcccatt gaagccggaa gtggatatcg acgctctttc gcctgacctt
     8581 aatgcgctga ccgctatcag taaacagatt ggctgcaatg gtttcttccc gttccagatc
     8641 cgtccaggca aaaacgaaac cgatggtcgt atgttctcgc ctgcgattgg tattgtggaa
     8701 gatccggtga ctggcaatgc caatggcccg atgggtgcat ggttggtaca tcacaacgta
     8761 ttgccccacg atggcaacgt gttgtgtgtt aaaggccatc aggggcgcgc attggggcgt
     8821 gacggcatga ttgaagtgac ggtgacaatt cgtgataacc aaccggaaaa agtcaccatt
     8881 tcaggcacag cggtgatttt gtttcatgcc gaatgggcaa ttgaaatctg aaatcgttgc
     8941 ctgatgcgct tcgcctgtca ggtctacaac cgggctaatg tatgccggat aaggcgttta
     9001 cgccgcatcc ggcaacctga cataaattat tttcccgcct ccggatgcgt atcaaacgcc
     9061 gccatcacca gcgctaactc atccacagta aagccatgtt tcgcatcatc cacgcccaga
     9121 ccaaactgtt cctgcatcac ggcatataac gacgccacat ccggtagatt tcgctgctcc
     9181 accgcgtgcc cattttcata atgggtaaaa tgaaaattgg tcagtgtcag cttgccgccg
     9241 tccggcaaat ggcggcacat cagcaaatga tggcgaaaat gcgactgcgg ccagtgcgcc
     9301 gaccagaaat tgcccatcac ataatcgctt tgttgctgct cgcagagatc gaagcggtac
     9361 atcggctgcc aatgctgatg atgattaaac tgcaacaccc aatcatcacc ctcctgcaac
     9421 aaccgatact ctccgtgtgg cgtggtctgc acgagatcgg aaactaaacg aatcggcgcg
     9481 gttagcgtct gcccaccgaa accgacatca gcaatccatt tttccccttc cagttccacc
     9541 aacagcaaac gatgggtgcg cggcggtaat gctggcggat ttgataacac tacgcgccct
     9601 aacaagctgc gaacgttaaa ccccagctcg cgtaacaccc gctcaaacac gccattctgc
     9661 tcaaaacagt aaccgccacg acgggctatc accagtttct cttccagcga ttgatcatca
     9721 agctgtattt ccctcggcag caaaacgtca aggttttcaa acggaatggt gcaattgtgt
     9781 ttcaggtgca atgcacgaag cgtatcaata ttgaccgcag cagctcccga ccagttaata
     9841 cgggcaaaat agtgattcag aatgggcgtc atcaggcgtt tcccttagtg gttttctcaa
     9901 cttataacct ggaatagacg ccgcaccggc tattttgtgc gatcgcggac atgcatcacg
     9961 tttgcaaatc gccaatcccg ttaatttcgt tacttattgc gttacggact acgcttaaaa
    10021 aaaccttctt cgcgaggcga taccatgagc cgattcatcc ccatagaatt acaccatgcc
    10081 agccgtctgt taaatcatgg cccaactgtc ttgatcacca gtttcgatga gcaatcccag
    10141 cggcgtaaca ttatggctgc agcctggtca atgccggtgg agtttgaacc tccacgcgtg
    10201 gcaattgtgg tagataaatc gacatggacc agggagttga ttgaacgtaa cggtaagttt
    10261 ggcatcgtta tcccgggcgt tgcagcaact aactggacgt gggcggtggg aagtgtgtcg
    10321 gtgcgtgaag aagataaatt taattgctat ggcattccgg tggtgaaagg tccggtgctt
    10381 ggtttgccac ttgttgaaga gaaatgcctg gcgtggatgg agtgccgcct gctacctgcg
    10441 acttctgcgc aacaaaaata cgacacgctg tttggcgagg tggtatcagc agcggcagac
    10501 gcacgggtat ttgtcgaagg ccgctggcag tttgatgatg ataagctcaa tacgttgcat
    10561 catttaggtg ctggcacgtt tgttactagc ggcaagcgtg ttacggcagg ttaagcatta
    10621 catgctgtaa ccgggtttct ttatcaaagc ctccatttgg ggctcgattt cggcatccca
    10681 gatagcttgc caagattctg gttgaatctg ctgtagagca atgcttatcg agctatcttt
    10741 actgttcaga tggcgaataa taacgtcggt aatatctgca gcaagtgctg ctttttgttg
    10801 ttcgtccagt tcacgcggaa agcatttaat gtcgatgtgc ggcatgatta cgctcctttt
    10861 gaaagatgtc agtaaaatta acacacattg tgaatgtcat gtgtccggta aagcgtttat
    10921 acgacaataa tgcccagggc aagattacga aagcccgctc cccgtaagga ctgacgccag
    10981 gtagtttctg cccatggctg cttttcgcat cttacgtctt aaccctgcct tgaatacctt
    11041 atcattcgtc aaaatattaa tagcaatgtg ccgtatccct gaaaataatt ctgctgcatt
    11101 tcctcttctt attttgcagt cgtcttcatt cattaccacg tccagacgcc aatgcagctt
    11161 attctccacg tgccagtggt ttcggattgc tgtggcgaac ttctctgcgg ttaaatcagc
    11221 agaactgata taatatctga ccgtcatttc tggctctttc ttttgttctg ctattatgga
    11281 ccgaaaggag actgccacgc ataatttctt cagccctttc cattcaaacg tgaaatcaat
    11341 aagttcatca gggacatcgc aaacaatatg aagacggatt tcttctctgc cgtgactctt
    11401 ttcactaatt gcgtaactgt cattctctgg attatttaat tctttcagcg gaaatttttc
    11461 ctcaaaggct ttatttagcc gcccctggtt tccttttacc gcgaataaat aatcacctcc
    11521 ctgtttttgt atcttctctg caatatcttt ctggcaaccc atcgcatcag ttgtgatgat
    11581 ttttccttta atatccagca tgttaagaag ttcaggaatg gctgtaatct cattggattt
    11641 ctcatccgtc ttaatctgcc cgagaatcag actgtgcatt gttgagaacg cactaatgac
    11701 atgaattgct cccctgcggc actcttgtca taagagtgcc ggagcgtttt tccatcaatt
    11761 gcaatgacgt ctttatcatc tgaagaatgg cagtcacgca tccagttaat aaagcactcg
    11821 tgaaattttg caggactgat acaggataca actctggcaa tagtatcgtg aacaggaata
    11881 ccattttcaa aatcaccata ttgcttcaaa aaatcgagat gtgtttcacc aaaatcctct
    11941 atatcttccc aaccctctgc accagaaatg acggcacaaa tagtcaacag tagaatatct
    12001 gacaatttat gttctacttt ccaggcttgt ctgtaatcgg ggataataga aatatgttcc
    12061 atcagttttt taagttccat tttgttctcc ttatttatgc aaggagtatt tgatcatata
    12121 taagctatga aaaatagttt taggtaataa ggaatatctc aattattgaa catttagcgc
    12181 gaattattta gtacaaaaag tgtacttccg atgatctttc cctgctccaa agtcttctaa
    12241 aatca
//
LOCUS       DADMKF010000118        12084 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.120, whole genome shotgun sequence.
ACCESSION   DADMKF010000118 DADMKF010000000
VERSION     DADMKF010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12084
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.120"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            202..768
                     /locus_tag="JNC24_003010"
     CDS             202..768
                     /locus_tag="JNC24_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HAY9159701.1"
                     /translation="MFKGQKTLAALAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLRLVNCDLENSSNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
     gene            819..3277
                     /locus_tag="JNC24_003011"
                     /pseudo
     CDS             819..3277
                     /locus_tag="JNC24_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
     gene            3292..4023
                     /locus_tag="JNC24_003012"
     CDS             3292..4023
                     /locus_tag="JNC24_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HAY9159702.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPAPEKNSDHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVNTNTGNYSGSQFYLGYMD
                     DYGALRMTTLNCSGQCHLQAVEAKK"
     gene            4020..5081
                     /locus_tag="JNC24_003013"
     CDS             4020..5081
                     /locus_tag="JNC24_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY9159703.1"
                     /translation="MSAGKGVLLVICLLFLPLKSALALNCYFGSSGGSVEKSEAIQPF
                     AVPGNAKLGDKIWESDDIKIPVYCDNNTNGNFESEHVYAWVNPYPGVQDRYYQLGVTY
                     NGVDYDANQGKSRIDTNQCIDSKNIDIYTPEQIIAMGWQNKICSGDPANIHMSRTFLA
                     RMRLYVKIREMPPHDYQSTLSDYIVVQFDGAGSVNEDPTAQNLKYHITGLENIRVLDC
                     SVNFSISPETQVIDFGKFNLLDIRRHTMSKTFSIKTTKSQNDQCTDGFKVSSSFYTEE
                     TLVEEDKALLIGNGLKLRLLDENASPYTFNKYAEYADFTSDMLVYEKTYTAELSSIAG
                     TPIEAGPFDTVVLFKINYN"
     gene            5188..6279
                     /locus_tag="JNC24_003014"
     CDS             5188..6279
                     /locus_tag="JNC24_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAY9159704.1"
                     /translation="MLYRYTKEKIMRERRMPVLQWGMLCVLSLLLSIGFLAVHLPAAL
                     LLGPMIAGIIFSMRGITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAI
                     LLVTLLSSAIVGWLLVRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYL
                     RVLFVAGAAVLVTRMMLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCMLRLP
                     SGTMLIPMLAGAVLQSGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQ
                     ILLSIFALLAICAGMAWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMA
                     MQTLRLFSILLTGPAIARFISTYAPKRSA"
     gene            complement(6276..7067)
                     /gene="nei"
                     /locus_tag="JNC24_003015"
     CDS             complement(6276..7067)
                     /gene="nei"
                     /locus_tag="JNC24_003015"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HAY9159705.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFSNDLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLRPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLDIPRFSYATRGLVDE
                     NKHHGALFRFKVFHRDGEPCERCGSIIEKTTLSSRPFYWCPGCQH"
     gene            complement(7103..7837)
                     /gene="pxpA"
                     /locus_tag="JNC24_003016"
     CDS             complement(7103..7837)
                     /gene="pxpA"
                     /locus_tag="JNC24_003016"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HAY9159706.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQIMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALILVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQRGALIENEEQALAQTLEMVQHGRVKSITGEWATVTAQTVCLH
                     GDGEHALAFARRLRSTFAEKEIVVAA"
     gene            complement(7827..8759)
                     /gene="pxpC"
                     /locus_tag="JNC24_003017"
     CDS             complement(7827..8759)
                     /gene="pxpC"
                     /locus_tag="JNC24_003017"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HAY9159707.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMKAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLKVGIGGLEGRLLKDGDRLPIGKSKRDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSSQSNRMGYRLQGQILKR
                     TTDRELLSHGLLPGVVQVPHNGQPIVLMNDAQTTDGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKARQDQQRYFEQLVWRLHNEN"
     gene            complement(8753..9409)
                     /gene="pxpB"
                     /locus_tag="JNC24_003018"
     CDS             complement(8753..9409)
                     /gene="pxpB"
                     /locus_tag="JNC24_003018"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HAY9159708.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAHCGFSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGVC
                     "
     gene            complement(9432..10175)
                     /gene="ybgI"
                     /locus_tag="JNC24_003019"
     CDS             complement(9432..10175)
                     /gene="ybgI"
                     /locus_tag="JNC24_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HAY9159709.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDV
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGLELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            10446..11927
                     /gene="dtpD"
                     /locus_tag="JNC24_003020"
     CDS             10446..11927
                     /gene="dtpD"
                     /locus_tag="JNC24_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HAY9159710.1"
                     /translation="MNKHASQPRTIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
BASE COUNT         2910 a         3211 c         2937 g         3026 t
ORIGIN      
        1 catgaataaa ccaccaaata tagcgcaccc ctataatttc caatacttta ttcatcaaac
       61 atttaattca taccatcaat taattacttt tacatttaat aaaattaata atatttttaa
      121 gaaaaactaa aaacattcga caatcaaata agatattctt gcaccgatat acttttttct
      181 tttctgttaa aggactcagt tatgtttaaa ggacaaaaaa cattggccgc actggccgta
      241 tctctgctgt tcacagcacc tgtttatgcg gctgatgaag gatccggtga aattcacttt
      301 aaaggtgaag ttattgaagc accgtgtgaa atacatcagg atgatattga taaagaggtt
      361 gaactcggtc aggtgaccac cagccacatt aatcagtcac atcacagcga tgccgttgct
      421 gtcgacctgc gcttagtcaa ctgtgatctg gaaaactcca gcaacggttc cggtggcaag
      481 atttctaaag ttgcagtcac ctttgatagc tcagcgaaaa ccaccggcgc agatccgatt
      541 ctcaacaaca ccagcacggg tgaagccacc ggcgtcggcg tacgtttaat gaataaagat
      601 caaagcaata tcgttttagg caccgctact ccagatatcg acctggctcc gacctccagc
      661 gaacaaacgc tgaatttctt tgcctggatg gaacaaattg atcaggctac acctgtaacg
      721 ccaggcgcag ttacggcgaa tgcaacctac gtgctggatt ataaataagt attactttgg
      781 cggcgctatc gccgcctttc ataaaatatt tatcaggtat ggacaccgtg aatatttatc
      841 gactctcttt tgtatcctgc ctggtcgtgg cgatgccttg cgcattggcg gtcgaattca
      901 accttaatgt tctcgataaa tcgatgcgcg accgcattga tatttcatta ttaaaggaaa
      961 aaggagtcat tgctcccggt gagtattttg ttagcgttgc ggtaaataat aaccaaatca
     1021 gtaacgggca aaagattaac tggcacaaaa atgacgataa aaccattccg tgcatcaatg
     1081 atttactggt cgataaattt ggcttaaaac ctgaagtccg tcagtcgtta ccattgataa
     1141 atcagtgcgt cgattttagc tcccgacctg aaatgctctt caatttcgat caagccaatc
     1201 agcaactgaa tattagtatt ccgcaagcct ggctggcgtg gcactcagaa aactggaccc
     1261 caccctccac atggaaagaa ggtgtcgccg gtatcctgat ggattacaac ttgtttgcca
     1321 gcagctaccg cccacaggac ggcagcagca gcactaacct gaacgcctac ggtaccaccg
     1381 gaattaacgc cggggcatgg cgcttacgta gtgattatca gttgaatcag actgatagcg
     1441 atgataacca tgaacagtca ggcggaatat cgcgcaccta tctttttcgt ccattaccgc
     1501 aattaggctc taaattaacc ctcggcgaaa cggattttag ttccaatatt ttcgacggtt
     1561 tttcttatac cggcgcggca ctggcaagtg acgagcgaat gttgccatgg gaactacgcg
     1621 gctacgcccc acaaattagc ggtattgcac agaccaatgc cacggtgacg atcagtcaat
     1681 caggccgcgt catttaccag aaaaaagtcc caccaggccc atttatcatt gacgacctta
     1741 atcagtctgt tcagggcaca ctggatgtca aagtgacgga agaagatggt cgggtgaaca
     1801 atttccaggt ttcggcagca tcgacgccct tcctgactcg tcagggacag gttcgctata
     1861 aactggccgc gggtcagcca cggccctcca tgtcacatca aactgaaaat gaaacctttt
     1921 ttagcaatga agtttcctgg gggatgctgt caaacacctc gctgtacggc ggcctgctgc
     1981 tttctggtga tgactaccat tctgccgcaa tgggtattgg gcaaaatatg ctgtggcttg
     2041 gtgcgctgtc gtttgatgtc acgtgggcca gtagccattt tgatactcag caggacgagc
     2101 ggggcttaag ctaccgtttt aattacagca aacaagtgga tgccactaac agcacgattt
     2161 ccctcgccgc ttatcgtttc tccgatcgtc attttcacag ctacgccaac tatctggatc
     2221 acaaatacaa cgacagcgat gcgcaggacg aaaaacagac gatcagctta ttgtgggcca
     2281 accgattacc ccactaaacc tcaatcttta cgccaacctg ctacatcaaa cctggtggaa
     2341 tgcagacgcc tccacgaccg ccaacatcac ggcaggtttt aatgttgata ttggtgactg
     2401 gagagatatc tctatttcga cgtcattcaa tacaacccat tacgaagata aagatcacga
     2461 caaccagatt tacctgtcga tttcgctccc cttcggtaac ggtggtcggg ttggctatga
     2521 catgcaaaac agtagccaca gcaccacaca ccgcatgtcg tggaacgata cgctggatga
     2581 acgtaatagc tggggcatgt ctgccggact gcaatccgac cgtcctgaca atggagccca
     2641 ggtgagcggt aactatcagc acctgagttc agcgggtgag tgggatattt ctggtaccta
     2701 tgccgccaat gattacagtt ccgtcagcag cagctggagc ggttctttca ccgcaaccca
     2761 atatggtgca gcgtttcatc gccgcagctc caccaatgaa ccacgcctga tggtcagcac
     2821 cgatggcgtg gcagatattc cggttcaggg caatctcgac tacaccaacc attttggcat
     2881 tgcggtggtg ccgttgattt ccagttacca gccttccacc gtggcggtga acatgaatga
     2941 cttacccgac ggcgtaacag ttgcagaaaa cgttatcaaa gaaacgtgga ttgaaggcgc
     3001 gataggttac aaatcactgg cttcccgttc cggtaaagac gttaacgtca tcattcgcaa
     3061 cgccagcggt cagtttcctc ccctcggagc ggatatccgc caggatgaca gcggcattag
     3121 cgtggggatg gttggcgagg aaggacatgc ctggttaagc ggagtcgctg aaaatcaaaa
     3181 gtttaccgtg gtctggggtg atagccagca ttgctcgctc catcttcctg aacatatgga
     3241 agacaccgca aatcgcctga ttttaccttg tcattaatta aggaaatgaa aatgacattt
     3301 atgaaaggac tacccctttt gttgttggtt gccagtctgt gcagccacgc tgcactacaa
     3361 cccgatcgca ctcgtattgt gtttaacgct aatgataaag ctaccagtct gcgggtggat
     3421 aatcgtagcg ataaactccc ctacctcgct tattcctggc tcgaaaatga aaaaggggag
     3481 aaaagcgatg atcttctggt cgccctgccg ccgatacaac gtctggaacc gaaagcaaca
     3541 acgcaggtac ggatagtaaa gcaagcctcg accacaaaat taccgggcga tcgcgaaacg
     3601 ctatttttct acaatatgcg tgaaattcct cccgccccgg aaaaaaacag cgaccatgcc
     3661 gttctgcaag tcgccattca gagtcgtatc aaggtgttct ggcgaccggc tgcattacgc
     3721 aagaaagcag gagaaaaggt cgaactgcag ttacaggtca gccagcaggg caaccaactg
     3781 acactgaaaa atcctaccgc gtattatctg accattgcct acctgggacg aaatgaaaaa
     3841 ggcgttctcc ccggcttcaa aaccgtgatg gttgcgcctt ttagtacggt caacacaaac
     3901 accggaaatt atagcggcag ccagttttat ctcggttata tggatgatta cggcgcgtta
     3961 cgcatgacga cgctcaactg cagcggacaa tgccatttac aagccgtgga ggcgaagaaa
     4021 tgagtgctgg caagggagtg ttgctcgtca tctgtctgtt atttctgccg ttgaagtctg
     4081 cgcttgccct gaactgctac tttggttcat ccggtggctc ggtagaaaaa tcagaagcga
     4141 ttcaaccttt tgctgtacca ggcaatgcca aactcggcga taagatttgg gaatctgacg
     4201 atattaaaat tcccgtctat tgtgacaaca ataccaacgg caattttgaa agtgagcatg
     4261 tttatgcctg ggtaaacccc tacccggggg tgcaggatcg ctattatcaa ctcggcgtaa
     4321 cttacaatgg cgttgattat gacgccaacc agggaaaaag ccgcatcgac actaaccagt
     4381 gtattgacag taagaacatt gacatttata cccctgagca gattatcgcg atgggatggc
     4441 agaacaaaat ttgctcgggt gatcccgcta atattcacat gtcgcgtacc tttctcgctc
     4501 gcatgcggtt atacgtcaaa atacgagaaa tgccgccgca tgattatcaa agtacgctta
     4561 gcgactatat cgtcgtgcaa tttgacggtg ccggtagcgt taacgaagac cctactgccc
     4621 aaaacctgaa atatcatatt actggtctgg aaaacattcg cgtgctggat tgcagcgtca
     4681 atttttccat ttcaccggaa acacaggtga ttgattttgg taaatttaat ttgctggata
     4741 ttcgtcgcca cacaatgtcg aaaacattca gtattaagac gacaaaatca caaaatgacc
     4801 aatgcaccga cggatttaaa gtcagttcct ccttttatac cgaagaaacg ttggttgaag
     4861 aagataaagc attgttgatt ggtaatggtc tgaagctacg tttgctggat gaaaacgcct
     4921 cgccttatac cttcaataaa tatgctgaat acgccgattt caccagcgac atgttggtct
     4981 acgaaaaaac ctatacagct gaactttcgt ccattgcagg tacccccatc gaggctgggc
     5041 ccttcgatac agtggtgctt tttaaaatta actataactg atttgccatg atgccctttg
     5101 cagccggaaa gggcatctct ttcagaaaat tgctgatttt ccccttttcc ttgattgctg
     5161 ttattttttc ctggcccggc tttctttatg ctgtatcgat acaccaaaga gaaaataatg
     5221 agggagcgaa ggatgccagt tttgcagtgg ggaatgttat gtgtgctatc acttctcctt
     5281 tctattggtt tcctcgcggt acatctcccg gcggcgctat tacttgggcc gatgatcgcc
     5341 gggatcatct tcagtatgcg cggaattact ctgcaacttc cccgctccgc ttttctcgcc
     5401 gcgcaggcca ttcttggctg catgattgcg caaaacctca ctggttcgat tctcaccacg
     5461 ctggccgcca actggccgat cgtgcttgct attttactgg tgacgctgct ttccagcgcc
     5521 atcgtgggtt ggttattggt gcgctatagc tcactgcctg gaaataccgg tgcctggggt
     5581 tcttctccag gcggcgcagc ggcaatggtc gctatggcgc aagattacgg cgcagatatt
     5641 cgcctggttg catttatgca atatctgcga gtgctgttcg tcgcgggggc cgctgttctg
     5701 gtcacccgca tgatgctggg tgataacgct gaagcggtta accagcaaat cgtctggttc
     5761 ccgccagtga gcattaatct cctgcttacg attttgctcg cagtcgttgc cggtacggcg
     5821 ggatgcatgc tgcgtcttcc ttccggcacg atgctcatcc cgatgctggc gggcgcagtg
     5881 ctccagtctg gtcagctcat caccatcgaa ctaccggaat ggctgctggc gatggcgtat
     5941 atggcaattg gctggcggat tggtcttggt ttcgataaac aaatcttact gcgggcacta
     6001 cgcccgctac cgcaaatcct gctgtcgatt tttgctctgc tggctatttg tgcgggtatg
     6061 gcgtgggggc tgacccggtt tatgcacatt gattttatga ctgcctacct cgccaccagt
     6121 cccggcgggc ttgataccgt ggcggtcatc gccgcaggga gcaatgccga tatggcgctt
     6181 attatggcga tgcaaaccct gcgcctgttc agtattttgc tgacggggcc agccattgcg
     6241 cggtttattt caacctatgc gccgaagcgg tcggcctagt gctggcagcc agggcaccag
     6301 taaaacgggc gagatgacag cgtggttttc tcaatgatgc tgccacagcg ttcgcacggt
     6361 tcgccatcac gatgaaaaac cttaaagcga aacagcgccc catgatgctt attctcatcc
     6421 accagccccc tcgtagcgta ggaaaatcga ggaatatcca gtaacgcgtg tgcgagtgca
     6481 tccagttgcg ccgcattgag atctttcgct ttatgatttc cagtcaaccc aacctgccag
     6541 aggatctcca cccgcaaata attgccaagc ccagccagaa acgcctgatc gagcagtaat
     6601 cctgcaaact gacggttacg aaagcgcggt gacaataacc gttcctttac cacctccggc
     6661 gtcagattcg gatccagcac atcgggacca acgcgttgta aaaacggatg cgtggtcagt
     6721 tgttccggcc tcaacatctc aatatccgag gcgctataaa gcagaatggt tttgtcagcc
     6781 gtttgcagtt ttacccgcaa tactcgcgtg gtctgcggct cttcgccggt atcaaccacg
     6841 cgccagacgc cgtaaagctg attatggctg tagagcgtta agtcgttgga aaaatgagtt
     6901 aacaacgcct taccacgcgt ttccacatgg gtaacgtgtt gaccgataag ttgtgattga
     6961 taagttttta actgcgggaa ggcaaaccag acatcagtta gtggtttgcc tttgatcgcc
     7021 gcctccaggt tatccgctgc acggcggatc tccgggcctt caggcatggt gatatccttt
     7081 tttcattctt tattaatcgg ggttatgctg cgacaacaat ctccttttcg gcaaatgtag
     7141 agcgtagtcg gcgggcgaac gccagtgcgt gctcgccgtc gccatgcagg cagactgttt
     7201 gcgccgtgac cgttgcccat tcgccggtga tacttttgac tctgccgtgt tgcaccatct
     7261 ctagcgtttg cgccaacgcc tgttcttcgt tttcaatcaa cgcgcctcgc tgacttcgcg
     7321 gcaccagcga gccgtcagcc tgataaccgc gatcggcaaa cacttcctcg cgcgttgtca
     7381 ggccatattg cttgcctgca cgaatcagct cgcttcctgc cagcccaacg agaatcaatg
     7441 ccggatcgca agcgtatacc gctctggcga tggcgtctgc cagttgtgct tctttcgccg
     7501 cctggttgta caacatgccg tgcggtttga catgacgcat tacgccgcct tgcgcacggg
     7561 caatcgttgc cagcgcgcca atttgataca gcgtctgggc gtaaacggtt tctggcggca
     7621 gctgcatggc gctgcgacca aaattttccc tgtcgggaaa actcgggtga gcgccaatcg
     7681 cgacaccatt ttttattgct tcacgcacgc aagcctgcat gatttgcgca tcgcctgcat
     7741 gaaatccaca ggcaatattg gcagaggaaa ccagcgttaa tagctctgcg tcgctggcgc
     7801 agccttcgcc cagatcggcg ttcaggtcaa ttttcattgt gcagccgcca cactaattgt
     7861 tcgaaataac gttgctgatc ttgccgcgct ttcagtgcct cttccagtga acactggaca
     7921 aaatgaatcg gctgaccgag cggaatttgc gccagatggt acatatcagc ctcaatgata
     7981 caggcaatac gcgggtaacc atcggtggtc tgtgcgtcgt tcatcaacac aatcggctgc
     8041 ccgttatgtg gcacctgcac cacgcccggt aacaaaccgt gagataacag ttcgcgatcg
     8101 gtggtgcgtt ttaaaatttg cccctgtaag cgatagccca tgcggttact ttgcgagcta
     8161 agctgccagg gcgaacgcca gaatgcatcc tgcgaggcgc gatcgaactc atgatattcc
     8221 ggccccggca aggcgcgaat gcggttgccc cacagcagct gtttaacgcc ctgcgcttcc
     8281 ataaaatcac gcttcgattt gccaatcggg agtcggtcac catccttcag taaacggcct
     8341 tccagcccgc caatccccac tttgagatcg gtgctgcatg accccattac cggcggaaca
     8401 tcaataccac ccgcgaccgc cagataactg cgcatcccgt gctgcgggcg tttaagcgtt
     8461 aaacgctggc ctgctttcat cggcaatcgc cagccggtcc agacggcatt atcatccagc
     8521 cgcgcttcgc aaccggcacc cgtcagagca aaccacccat cagtttcgaa ctcaaccgtt
     8581 aactgaccga gcgtgatctc cagcgcgggg gcattggcgt cattacccac cagcaggtta
     8641 gcaatgcgta acgcaggcat atccagtgcg ccgcagtggc tgattcccga ctggcgaaaa
     8701 ccgtgacgac cgccatcctg cacagtggta tacatgcccg cacgaataat cttcagcata
     8761 ctccctcctt ctgcggtaca aagcgcacgc tgtctcccgg acgtaataag atgggttcgt
     8821 cacgcgccgg atcaaacagg ctgagtgagg tatgaccaat caactgccag ccacccggcg
     8881 ttgccagcgg ataaacacca gtctgcggcc cgccgatccc gacagaacct gccggaacga
     8941 gcaagcgcgg ttcagcgcgc cgtggcgtgt gtagttgttc cggcaaactc ccgagatacg
     9001 ggaagcccgg ttgaaaacct aaaaaccaga ccacgtattc cacggaggag tgcaattcaa
     9061 caacctgttt ttcgctaaac ccgcaatgcg ccgcgaccac cgccaaatcc ggtccgcctg
     9121 caccaccgta aaccaccgga atttcaataa agcgagactc aggttccagg gcctcgctct
     9181 cctcccacca gcgttgcaaa cgctctatgg catccagcgc cagcgactca ggattacgca
     9241 aaatcaccgt gatattgttc atgccgggaa tggcttcaac cacattcggc atatccacca
     9301 gacgctgcgc cagtcgccag atccgtttct ggctagccag cgtcaccggc ggttccagtt
     9361 ccagcactac cgccgtttca cctatcagat aacaacgcgc tcgttgcact ttcgatccct
     9421 ctgattattc gttatgcagg attaggaata tcaataaagg tcacatcaag atcggtattt
     9481 tcattcagcc actcgctcaa tgcgcgaata ccaccacgtt cagtggcatg gtgacctgca
     9541 gcataaaaat gcaacccctg ctcgcgggct gaatgaatgg tctgttcaga aacttcgcca
     9601 gtaataaaag catccacgcc aaaacgcgcg gcgctatcga taaaactttg cccgccgccc
     9661 gtgcaccagg cgacgcgctg gaccacctca ggtccggtat cgccacacca taacggctta
     9721 cgtcccagac gcgcttcaat ccaggaggct agttccagtc ccggcacagg catggtcagc
     9781 tctccccacg gcaccaacgg ctcaatttcg cccatgaccg tgatccccag taacgccgcc
     9841 agctgtgcgt tattgcccag ctcaggatgt acgtcaagcg gcaaatgcca gccatacagg
     9901 ttgatatcat tcgccagcaa cgtttttaaa cggttacgct tcattccgcg aatgaccgga
     9961 gactctcctt tccagaagta gccatgatgc acaatgaccg catccgcgcc cagacgcact
    10021 gcctcatcga gcaaagcctg gctggcggtg acaccggtaa caattttttg caccgtctct
    10081 ttgccttcta cctgcaaacc attcggcgcg taatcactaa tcgccgcgct gttcagtttt
    10141 tcgttaatca gttgttccag ttcggtgttt ttcatcttcg cgccttccgt attctgatag
    10201 ctacaccgtt aacatagcgt tacctcgcct gttcgtcgat agccaatctt ttgacatatt
    10261 aagaaacaaa cccgacgcca aaagacgcaa attgtttata tcatcacgct tcagctaatg
    10321 tatatttatg caatcagaca gcatattgca taaataatca gatctgtatc aaagttttaa
    10381 ctttaaggaa acacaagcac gccgcgcaac tattacgtga cgttgcagtc aattgtggaa
    10441 ttattatgaa taaacacgca tcacaaccgc gcactattta ctatgtcgtt gcgctgcaaa
    10501 tctgggaata ttttagcttt tacggcatgc gtgccctgct gattctctat ctcaccaatc
    10561 aactaaaata caacgatact cacgcctacg agttatttag cgcctactgt tcgctggtgt
    10621 atgtcacgcc aatcctcggt ggctttttgg cggataaagt tctcggcaat cgcatggcgg
    10681 tgatgctggg ggcgttgttg atggcgatcg gtcatgtggt gctgggtgcc agtgagatcc
    10741 atccgtcatt cctctatctg tccctggcga ttatcgtttg tggctatggt ctgtttaaat
    10801 ccaatgtcag ttgcctgctc ggcgagctgt atgagccaac cgatccgcgt cgtgatggcg
    10861 gtttctcgct gatgtatgca gcgggtaacg tggggtctat tatcgcacct atcgcctgtg
    10921 gttacgccca ggaagagtac agctgggcga tgggctttgg cctggcggcg gtgggtatga
    10981 tcgcgggtct ggtcattttc ttatgtggca atcgtcattt cacacatacc cgcggcgtta
    11041 ataaaaaagt actgcgtgca acaaactttc tcctgcctaa ctggggatgg ctgctggttc
    11101 tgctggtggc aacgcctgcg ctgattaccg tactgttctg gaaagagtgg tcggtatacg
    11161 ccttaattgt cgcgactatt attggcctgg gtgtactggc aaaaatttat cgcaaagcag
    11221 aaaaccagaa acagcgtaag gagctggggc tgattgtgac gctcaccttc ttcagtatgt
    11281 tgttctgggc cttcgcacaa cagggcggca gctcaattag cctttatatc gaccgcttcg
    11341 ttaaccgcga tatgtttggt tataccgttc cgactgctat gttccagtcg attaatgcct
    11401 tcgcagttat gctatgcggt gtgttcctgg cgtgggtggt taaagaaagc gtcgcaggta
    11461 atcgtaccgt gcgcatctgg ggaaaatttg ctcttggcct tggcctgatg agcgccggat
    11521 tctgcattct gaccttaagc gcccgctggt ccgcaatgta tggtcactct tctctgccac
    11581 tgatggtgtt aggcctcgcg gtgatgggct ttgcggaact gtttatcgac ccggttgcca
    11641 tgtcgcaaat tacgcgcatt gaaattccgg gcgtgaccgg cgtattaacc ggcatctaca
    11701 tgctgctttc tggcgcgatc gcgaactatc tggcgggcgt gattgccgat cagacatcgc
    11761 aggcttcgtt tgatgcttcc ggggcgatca actactccat caatgcatat attgaagtat
    11821 ttgatcaaat tacctggggc gcactggcgt gtgtaggact ggtactgatg atttggctgt
    11881 atcaggcgct gaaattcaga aaccgcgcgc tggcgctgga gtcataatag gccagcaccg
    11941 atgcctgatg cgacgcttga cgcgtcttat caggccggtg atgctgccaa cttactgatt
    12001 tagtgtatga tggtgttttt gaggtgctcc agtggcttct gtttctatca gctgtccctc
    12061 ctgttcagct actgacgggg gggt
//
LOCUS       DADMKF010000119        12037 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.121, whole genome shotgun sequence.
ACCESSION   DADMKF010000119 DADMKF010000000
VERSION     DADMKF010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12037
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.121"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            9..323
                     /locus_tag="JNC24_003021"
     CDS             9..323
                     /locus_tag="JNC24_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000937361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAY9159711.1"
                     /translation="MLPPLFIMLAYLNLRAKLDHLPRDFRMGSRRTGIIVVSMLIAIF
                     AVGFVASTFPTGANILTIIFYNVGGIVIFLGFAWWKYSKYIKGLTAEERHIEATPASN
                     VD"
     gene            457..1534
                     /gene="ygjJ"
                     /locus_tag="JNC24_003022"
                     /pseudo
     CDS             457..1534
                     /gene="ygjJ"
                     /locus_tag="JNC24_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417550.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
     gene            1551..3902
                     /gene="ygjK"
                     /locus_tag="JNC24_003023"
     CDS             1551..3902
                     /gene="ygjK"
                     /locus_tag="JNC24_003023"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAY9159712.1"
                     /translation="MKIKTILTPVTCALLISFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVDFTLEAYSIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKITSNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTAQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWLSPGGAVKFNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDSVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKAWLAEMYSKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGDKEETQSGLNNYARVVEKGLYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVANGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPTTQ
                     FYYDVRIEDKPLANGCAGKPIVERGKGPEGWLPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            4128..6146
                     /locus_tag="JNC24_003024"
     CDS             4128..6146
                     /locus_tag="JNC24_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HAY9159713.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFAHCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRAVRERVGNDFIIIYRLSM
                     LDLVEDGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADMVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYHRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIARFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(6191..6607)
                     /gene="higA"
                     /locus_tag="JNC24_003025"
     CDS             complement(6191..6607)
                     /gene="higA"
                     /locus_tag="JNC24_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HAY9159714.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLADLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(6604..6918)
                     /locus_tag="JNC24_003026"
     CDS             complement(6604..6918)
                     /locus_tag="JNC24_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mRNA interferase HigB"
                     /protein_id="HAY9159715.1"
                     /translation="MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAV
                     FPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKG
                     KK"
     gene            complement(7203..8339)
                     /gene="rlmG"
                     /locus_tag="JNC24_003027"
     CDS             complement(7203..8339)
                     /gene="rlmG"
                     /locus_tag="JNC24_003027"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HAY9159716.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     RGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRSEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            8424..8927
                     /locus_tag="JNC24_003028"
     CDS             8424..8927
                     /locus_tag="JNC24_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HAY9159717.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPITKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            9004..9687
                     /locus_tag="JNC24_003029"
     CDS             9004..9687
                     /locus_tag="JNC24_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HAY9159718.1"
                     /translation="MQPRLLLRICFSRRTLKITCLLLLVAGATIFIADRVMVNASKQL
                     TWSDVNAVPARNVGLLLGARPGNRYFTRRIDTAAALYHVGKVKWLLVSGDNGRKNYDE
                     ASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRAIWL
                     AKQYGIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHRQPKYLGPSVMIGPF
                     SEHGCPAKE"
     gene            9748..10752
                     /locus_tag="JNC24_003030"
     CDS             9748..10752
                     /locus_tag="JNC24_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAY9159719.1"
                     /translation="MEYPRVMIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQ
                     AQHFANDFSVEHLFTSLEAMAESDAIDAVYIASPNALHFSQTQLFLSHKIHVICEKPL
                     ASNLAEVDAAIACARENQVVLFEAFKTASLPNFHLLRQALPKVGKLRKVFFNYCQYSS
                     RYQRYLDGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAH
                     GVVVMDYGDFSVTLQHSKVSDSVLESEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDL
                     TQPQHINTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKL
                     "
     gene            11035..12000
                     /gene="alx"
                     /locus_tag="JNC24_003031"
     CDS             11035..12000
                     /gene="alx"
                     /locus_tag="JNC24_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HAY9159720.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGE"
BASE COUNT         2862 a         3093 c         3225 g         2857 t
ORIGIN      
        1 cagcgtccat gcttccgccg ttattcatca tgctggctta cctgaattta cgcgccaaat
       61 tagatcacct gccacgcgat ttccgtatgg gctcccgccg caccggtatt atcgttgttt
      121 caatgctgat tgcgatattt gccgtagggt ttgtcgcttc gacattcccg actggcgcga
      181 atattctgac catcattttt tataacgtcg gcggtattgt tatcttcctt ggctttgcgt
      241 ggtggaaata cagtaaatat ataaagggat taacggctga agagcgccat attgaagcga
      301 cgccagccag caatgttgat taagcagaat aataaaaaat gagcaggcat tttccctctt
      361 cctggatgag gagagggaaa atagtttctg cctttatatt tttataatta caacgataaa
      421 aggctgtact ttttctttag ctcatggatt aacacaatga aattaatcac tgcaccatgc
      481 agagcattac ttgctctgcc gttttgctac gccttttctg cggcaggaga agaagcacgt
      541 ccggcagaac atgacgacac aaaaacaccc gcaattacct cgacatcttc tccttcattt
      601 cgtttttacg gcgaattagg gattaggggg tggtggatat atggatttag agggtgagaa
      661 taaacataaa tacagcgacg gtacctatat tgaaggtggc ctggagatga agtacggctc
      721 ctggttcggc ctgatttacg gcgaaggctg gaccgtgcag gccgaccacg acggcaatgc
      781 ctgggtgcca gaccatagct ggggtggttt cgagggcgga attaaccgtt tctatggcgg
      841 ttatcgtacc aatgatggca ccgaaatcat gctcagtctg cgtcaggatt cctcgctgga
      901 tgacctgcaa tggtggggcg atttcacccc cgatctgggc tacgtcattc ccaatacccg
      961 cgacattatg actgcgctga aggtacagaa cttaagcggc aactttcgtt atagcgtcac
     1021 cgcgactcct gccggacatc atgatgaaag caaagcctgg ctacattttg gcaaatacga
     1081 tcgctatgac gacaaataca cctatccggc aatgatgaac ggttacatcc agtatgacct
     1141 tgccgaaggc atcacctgga tgaacggtct ggaaatcacc gacggcacag gacagctcta
     1201 tctcacgggc ctgctaactc ctaactttgc cgctcgcgcc tggcaccata ccggacgcgc
     1261 cgacgggctg gacgtaccgg gaagtgaaag tgggatgatg gtgagcgcca tgtatgaagc
     1321 gttaaagggc gtttatctct ccaccgctta cacctacgcc aaacatcgcc ctgaccacgc
     1381 tgacgatgaa accacctctt tcatgcagtt tggtatctgg tacgaatacg gcggcggacg
     1441 tttcgccacg gcttttgata gccgcttcta catgaaaaat gcctctcacg atcccagcga
     1501 ccaaatcttc ctgatgcaat atttctactg gtaataagga ctgtaatttc atgaaaataa
     1561 aaactatttt aacgccagta acctgcgctc tgctgataag tttttccgcc catgccgcta
     1621 acgccgacaa ttataaaaac gtgattaacc gtactggcgc gccgcagtac atgaaggatt
     1681 acgattacga cgatcaccag cgttttaatc cgtttttcga tctcggagcc tggcatggtc
     1741 atctgttgcc agacggccct aacaccatgg gcggctttcc gggcgttgcg ctgctgacgg
     1801 aagagtacat caactttatg gccagcaatt tcgaccgcct gaccgtctgg caggacggca
     1861 agaaagtcga ctttacgctg gaggcataca gtattcccgg tgcgctggtg caaaaactga
     1921 cagcaaaaga tgtgcaagtc gaaatgactc tgcgcttcgc cacgccgcgc acgtcactac
     1981 tggaaaccaa aatcaccagc aataaaccgc tggatctggt gtgggacggc gaactgctgg
     2041 aaaaactgga agcgaaagaa gggaaaccgc tttccgataa aaccattgct ggcgaatacc
     2101 ctgactatca gcgcaaaatc agcgccaccc gtgatggcct gaaagtcacc tttggcaaag
     2161 tgcgcgccac ctgggatctg ctgacctccg gcgaatcgga atatcaggtg cataaatccc
     2221 tgccggtgca gactgaaatc aacggtaatc gctttaccag taaggcgcat atcaacggtt
     2281 cgaccacgct ctacaccacc tattcccatc tgctgaccgc tcaggaagtt agcaaagagc
     2341 aaatgcagat ccgcgatatt ctggcgcgtc cggcgtttta tctcaccgcc tcgcagcaac
     2401 gctgggaaga atacctgaag aaagggttaa ccaatccgga tgcgacgccg gaacagacgc
     2461 gcgtcgcggt gaaagcgatc gaaacgctca acggtaactg gctctcaccg ggcggtgcgg
     2521 tgaaatttaa caccgttaca ccgtcggtga ccgggcgctg gttctccggc aatcagacct
     2581 ggccgtggga tacctggaag caggcgtttg cgatggcgca tttcaatccg gacatcgcca
     2641 aagagaatat ccgcgcggtc ttctcctggc agatccagcc tggcgatagc gtgcgtccgc
     2701 aggatgtggg ctttgtcccc gacctgatag cgtggaacct tagccccgag cgtggtggcg
     2761 atggcggcaa ctggaacgaa cgtaatacca aacccagcct tgccgcctgg tcggtgatgg
     2821 aagtgtacaa cgtcacccag gataaagcct ggttggcaga gatgtactcg aaactggtgg
     2881 cctatcacga ctggtggtta cgtaaccgcg atcacaacgg caacggcgtg ccggaatatg
     2941 gcgcgacccg cgacaaagcc cacaacactg agagcggcga gatgctgttt acggtgaaaa
     3001 aaggcgataa agaagagacg cagtctgggc tgaacaacta cgcccgcgtg gtggagaaag
     3061 gcctgtacga cagtctggaa attccggcac aggttgctgc atcgtgggaa tcggggcgtg
     3121 atgatgccgc cgtctttggg tttatcgaca aagaacagct ggataaatat gtcgctaatg
     3181 gcggcaaacg tagcgactgg acggtgaaat tcgccgaaaa ccgcagtcag gacggaacgt
     3241 tgctgggcta ctcgctattg caggagtcgg tggatcaggc cagctatatg tacagcgata
     3301 accattatct ggcggagatg gcaaccatcc tcggtaagcc agaagaggcc aaacgctatc
     3361 gccagttggc acagcagctc gcggactaca tcaacacctg tatgttcgac ccgactaccc
     3421 agttctacta tgacgtacgt attgaagata aaccactggc gaacggctgc gcgggcaaac
     3481 cgattgttga gcgcggtaaa gggccggaag gctggttgcc gctgtttaac ggtgcggcaa
     3541 cgcaggccaa cgccgacgcg gtggtgaagg tgatgctcga tcctaaagag ttcaacacct
     3601 ttgtcccgct gggaacggcg gcgttaacca atccggcttt tggcgctgat atctactggc
     3661 gtgggcgcgt atgggtggat cagttctggt ttggtctgaa agggatggag cgttacggtt
     3721 atcgtgatga tgccctgaag ctggcggata cgttcttccg gcacgccaaa ggattaaccg
     3781 ccgatggccc aattcaggag aattacaacc cgctgactgg cgcacagcaa ggcgcaccaa
     3841 atttctcctg gagtgccgct catttgtata tgttgtataa cgattttttc cgtaagcagt
     3901 aatttgcaac ggctggcgga tgcggcgtga acgccttatc cgccctacat gtgcaatccc
     3961 ccaggccgga taaaacgcgg caagcgttgc atccggcgtt accaccgggc gtattctttt
     4021 tgaatcccat cacaaacccc gcactcccct tttccctttt ctccatcgac ggctaaatta
     4081 gaactcatcc gaccacataa caattatttt acatactgga caattttatg agctatccgt
     4141 cgctgttcgc cccgctggat ttaggtttta ccacgttaaa aaaccgcgtg ttgatgggct
     4201 caatgcacac cgggctggag gaatacccgg acggtgccga gcggctggca gcgttttatg
     4261 ccgaacgcgc ccgtcacggc gtggcgctga ttgtcagcgg cggcattgca ccagatttaa
     4321 caggcgttgg catggaaggt ggcgcaatgc tcaacgacgc cagccagatc ccacaccatc
     4381 gcaccattac cgaagcggta catcaggaag gcggcaaaat agccctgcaa attttgcata
     4441 ccgggcgcta cagctaccaa ccgcatctgg tcgctccctc cgcattgcag gcccccatca
     4501 accgtttcgt tcctcacgaa ctcacccatg aagagatcct gcaactgatc gacgatttcg
     4561 cccactgcgc gcaactggca cgggaggcag gatacgacgg tgtagaggta atggggtccg
     4621 aagggtattt gatcaacgaa tttctgacgc tgcgcaccaa tcagcgtagt gaccagtggg
     4681 gcggcgatta ccgcaaccgg atgcgatttg ccgtagaagt agtgcgtgcg gtgcgcgaac
     4741 gcgtcggcaa cgacttcatt attatctacc gactgtcgat gctcgacctg gtcgaagacg
     4801 gcgggacttt tgccgaaacg gtagagctgg cgcaggccat tgaagcggcg ggcgcgacca
     4861 ttatcaacac cggcattggc tggcatgaag cacgtattcc gaccattgcc acgcccgtgc
     4921 cgcgcggcgc atttagctgg gtcacgcgca aattgaaagg ccacgtctcg ctgccgctgg
     4981 taaccaccaa ccggattaac gatccgcagg ttgccgacga tattctctcg cgcggcgatg
     5041 ccgatatggt atcgatggca cgaccgtttc ttgctgatgc ggaactgctg tcaaaagcgc
     5101 aatcggggcg agccgatgag atcaacacct gcattggctg caatcaggcc tgcctcgatc
     5161 agatcttcgt tggcaaagtc acctcgtgcc tggtgaatcc tcgcgcctgc cacgaaacca
     5221 aaatgccaat ccttcctgcc gtgcagaaaa aaaatctggc ggtggtcggt gcgggacctg
     5281 ctgggctggc gtttgccatt aacgcggcgg cgcgtgggca tcaggtaaca ttgtttgatg
     5341 cccacagcga gatcggcggg cagtttaata tcgccaaaca gatccccggc aaagaggagt
     5401 tttacgaaac gctgcgctat taccaccgga tgatcgaagt gacgggcgtg acgctgaaac
     5461 tcaatcacac cgtgacggcg gatcagttac aggcgttcga tgaaacgatc ctcgccagtg
     5521 ggatcgtgcc gcgcgttccg cctatcgacg ggatcgatca tccgaaggta ttgagttatc
     5581 tcgatgtact gcgcgacaaa gcgccggtag gcaacaaggt tgccatcatc ggttgtggcg
     5641 ggattggttt tgatacggcg atgtatttaa gtcagccggg cgaatccacc agccagaata
     5701 tcgccaggtt ctgtaatgag tgggggatcg acagcagcct acaacaggct ggtggcttaa
     5761 gcccgcaggg aatgcagatc ccgcgtagcc cacggcaaat tgtgatgctc cagcgcaaag
     5821 ccagcaaacc gggacagggg ttaggcaaaa ccaccggctg gatccatcgc accaccctgc
     5881 tctcgcgggg tgtgaaaatg atcccaggcg taagttatca gaagattgac gatgacgggc
     5941 tgcatgtggt gatcaacggc gaaacgcagg tattagcagt ggacaatgtg gtgatctgcg
     6001 cagggcaaga gccaaaccgc gcgctggcgc aaccgctgat tgatagcggg aaaactgtgc
     6061 atttaattgg cggctgcgat gtggctatgg agctggacgc acgacgggca attgcccagg
     6121 gaacacggct ggcgctggag atttaaatcg ttattttaag cgccggatgc aacgcatccg
     6181 gcacgtcgcg ttaatcaata aacaaggcgg gagaaatgcc gaatcgcgtt gccaattttt
     6241 tagcgtgttc cagcgttaat ttcctcttcc cgctcaaaac gcgtgacacc atagatttac
     6301 tgccaatttc cggcagatcg gcaagggtta aaccatattg atccataagg gtacgaatca
     6361 cggctatacc gccaggcatg gcttgagcca tggcattaaa ttccgcaaat tcgggcgctg
     6421 attcttccca cgcggttatt ttggcacaca ccagatccag caaggggttt tcaggatcgt
     6481 tgagcagcag atgatctacc agttccagcg cctgggtgta ttgttcctcg ttctgaatac
     6541 ccgccagaaa aggtgccacc gcagttagct tttctcctgc ttgcaagatg tcggcaatcg
     6601 caatcatttt ttccccttag tacgatgaac agcggtaaag aaatcgtatt ctttatgcgt
     6661 cataacttca cgtatgtagc acttttgcga ttcaaaaaag accattgcta caacacgtaa
     6721 ttcattgccc ccaacattga aaacataatg cttatccaga tatttgaagt tatccagaga
     6781 tgggaatact gcttttaatg actcaggttt tttgaaatat cccttagcaa tcgtgttccc
     6841 cagagccacc aactccgttt tatgttgcgg gtatttttcc gcagcatctt tcaatgcttt
     6901 ttgagttatc aggtgcattc ttcatcacgt ccgttgacaa attggcaata tgataacatc
     6961 cgttgacaga ttggcaacgg atgaatttat ctgtgtgacg gaagaataaa cgtagaaaaa
     7021 cgctaaacaa gagaatgtag aggatggtga gagggatttt ttgttttatg gaagcgcgat
     7081 tccagaactg ggcagacatt gccggatgcg acgcttgccg cgtcttatcc ggcctgggag
     7141 taacgtgctg aacgtaggcc ggataaggcg ttaacgccgc atccggcagg taactcccaa
     7201 tcttaccgac gacgccccag cttcactgct ttcagcacca caaatttatt attcgtggcg
     7261 atggtggtgc agttgccgaa aatcttcttc agtttatgga agtaatccag gtgacggttg
     7321 gcaacaatat acagctcgcc gttgattttc aggcagcggc gggcatggtg gaacatctcc
     7381 caggcgacgt tatcggtcag cgcatgttgt tggtgaaacg gcgggttgca gagcacagca
     7441 ttaaagcgga aaggctccac gccggagagc gcgttgttga tcataaactc gctgcgatct
     7501 aacgcctctg gcatgttggt ttcaacgttc aaacggctgg aagcaaccgc catcggcgat
     7561 tcatcgacaa acaccacttt cgcctgaggg tttttatcaa gcagcgtcag accaataacg
     7621 ccattaccgc aaccgagatc gacaatctca ccttcgagat tctctggcag atgttgcata
     7681 aagaagcgtg cgccaatatc cagcccggtg cgggagaaga cattcgcatg gttgtggata
     7741 gtccagtcag taccttccag cttccagcta acggtctgcg gcgcatcggc cagcggcggt
     7801 tcattgaaag tgcaattaat caggcgcgct tttttccatg ccagcgtagt ggtggtcggg
     7861 ccgagcactt tctcgaacag ctccagcgtg gaagtgtgaa tgtcacgggc tttggcaccg
     7921 gcaataatac gtgtatccga cgtgaccact ttgcgcagcg cacgcagttg ctgttccagc
     7981 aatgccagtg ttttcggcac tttgatcagc accacacccg gctgttgcgg gtagtcggcg
     8041 gtgctgtcga gaaacttcac gctcgactca tcaatcccgt tgaggcgtaa attctcgcgc
     8101 gtcgccagtt cactgatgta tgagtcgcca atgctgtacg gcttatgttc cgccagcgcg
     8161 cagcttaacg caccaaaggc atcattcagg atcaacaccg ggccgcggat ttctgtgtcg
     8221 tccaactgtt gcagcaaata ttcatccgcc gcttcccacg cctgtagcgg gttaacgtca
     8281 tccgtcgccg gaaaacgttg tagtgtcagt gaacggaaac cgttgtctaa gtggctcatc
     8341 ggccctcctg aatgataaaa tttcgacgtt gcccctgaaa agggtgcgtg agtataccct
     8401 ttttctcttt ttcgtgggcg gttatgagca atcttactta tcttcagggt tatcccgagc
     8461 agctactttc ccaggtgcga acgctgatta acgaacagcg tctgggcgat gtgctggcaa
     8521 aacgctatcc gggaacacac gactacgcca ccgataaagc cctctggcaa tatacccagg
     8581 atctaaaaaa tcagtttctg cgcaatgccc cgccgatcac taaagtgatg tatgacaata
     8641 agatccacgt actgaaaaac gcgctcggat tacataccgc tgtttctcgt gtgcagggcg
     8701 gcaagctgaa agcaaaagca gagatccgcg tcgctactgt gtttcgcaac gcgccggaac
     8761 cgtttttacg catgatcgtg gtgcacgaac tggcgcacct gaaggagaaa gagcataaca
     8821 aagcgtttta ccagttgtgc tgccatatgg agccgcagta ccaccagctt gagttcgaca
     8881 cccggctgtg gctaacgcag ttgtcgcttg ggcaggacaa aatctgaaaa tgatgtcagg
     8941 cgttatcttt tctttcttta gcgcctaaaa tcgacctccc ccctttcgtt aaggataacg
     9001 accatgcagc cccgccttct tctccgtatt tgtttttctc gccggactct aaagattacc
     9061 tgcttactgt tacttgtggc aggagcgacc atttttatcg ccgatcgcgt aatggtcaac
     9121 gccagcaagc agctgacctg gagcgatgtc aacgcagttc cggcgcgtaa cgtgggttta
     9181 ttgctggggg cgaggccggg taatcgctac tttacccgac gtattgatac cgcagcagcg
     9241 ttgtaccacg tcggaaaagt gaaatggctg ttagtcagtg gcgataacgg acgtaaaaat
     9301 tacgatgaag cctcaggtat gcagcaggct ttgatcgcta aaggcgtacc ggcgaaagtg
     9361 atcttctgcg actatgccgg attctcaacg cttgattcag tggtgcgtgc caaaaaggtg
     9421 tttggggaaa accatatcac cattatctcg caagaatttc ataaccaacg cgccatctgg
     9481 ctggcaaaac agtacggcat cgacgctatc gggtttaacg cgccagacct taatatgaag
     9541 cacggttttt atactcaact gcgggagaaa ctcgctcgtg tcagcgcggt gattgacgcc
     9601 aaaatcctcc atcgccagcc gaaatatctc gggccatcgg tcatgattgg gccgtttagt
     9661 gagcatggct gtccggcgaa agaataatgc gtatctgcgc acgtcgaaga tgaaaaaggc
     9721 gtgctacatt gacgacagaa tccctttatg gagtatccac gcgttatgat acgtttcgct
     9781 gtgattggta cgaactggat cactcgccag ttcgtcgagg ccgcccatga gagcggtaaa
     9841 tacaagttaa ccgccgtata ttcccgcagc cttgaacagg cccagcactt cgccaatgat
     9901 ttttctgtcg agcatctgtt tacctcgctg gaagcgatgg cggaaagcga tgccattgac
     9961 gcggtgtata ttgccagccc gaatgccctg cacttttccc agacacaact tttccttagc
    10021 cataaaattc atgtgatttg cgagaaacca ctggcgtcga atctggcgga agtggatgcc
    10081 gccattgcct gtgcgcggga aaatcaggtg gtgctgtttg aggcatttaa aaccgccagc
    10141 ctgccgaact ttcatttgct gcgccaggcg ctgccgaaag tcggcaaatt gcgtaaagtc
    10201 tttttcaact attgccagta ttcctcgcgg tatcaacgtt acctggatgg tgagaatccc
    10261 aacaccttta atccggcatt ctctaacggt tcaattatgg atatcggctt ttactgcctg
    10321 gcgtcggcgg tggcattatt cggtgaaccg aaaagcgtgc aggcaaccgc cagtttgttg
    10381 gcaagcggcg tagatgccca cggcgtggtg gtgatggatt acggtgattt cagcgttacg
    10441 ttgcagcact caaaggtcag tgattctgtc ctggaaagcg agattcaggg cgaagcggga
    10501 tcgctggtga ttgaaaaact gtctgaatgc cagaaagtgt gcttcgtgcc gcgtggcagc
    10561 cagatgcagg atctcaccca gccgcagcat attaatacca tgctctacga agcagagctg
    10621 tttgctaccc tggtggatga gcatctggtg gatcatccag ggctggcggt cagtcgcatc
    10681 accgccaaac tgctgactga gatccgccgc cagactgggg tgatttttcc ggcagatagc
    10741 gtaaaactat aattgccaaa gtaaaacagt gtaaaaggta tgtaacagac cattgactgg
    10801 ctgaatggtc tgtcatactt tgttacctgc aaaggggagt aacttcattg ccggtcgatc
    10861 gtcattacga tgtgtgaaaa accacatccg gtcaccgggc aacccgaaag gaatacgcag
    10921 acgtattcct tttttgttgt aagtgagacc ttgccggaag gcgaggtcta tgcataaaaa
    10981 gcagcggctg acgtcttccg acgttggccg tttttttatg tgtaaggaac ttctatgaat
    11041 actgtcggca cgccgttgct atggggcgga ttcgctgttg ttgtcgccat tatgctggct
    11101 atcgacctgt tgttgcaggg gcgtcgtggg gcacatgcca tgaccatgaa acaggctgcg
    11161 gcctggtcgc tggtctgggt gacgctgtcg ttactgttta acgccgcttt ctggtggtat
    11221 ctggtgcaaa ctgaaggtcg cgccgttgcc gatccacagg cactggcctt tctcacgggt
    11281 tatctgattg agaaatcgct ggcggtcgat aacgtctttg tctggctgat gttgttcagc
    11341 tatttctctg ttccggcggc attacaacgc cgcgtgctgg tgtatggcgt gctcggggcg
    11401 attgttctgc gtaccatcat gatcttcact ggcagctggc taatttcgca gttcgactgg
    11461 atcctgtata tcttcggtgc cttcctgctg tttaccggcg tgaagatggc gctcgcccat
    11521 gaagatgaat caggcattgg cgacaagccg ctggtgcgct ggctacgcgg tcatttgcgc
    11581 atgaccgaca ccatcgacaa cgagcatttc tttgtgcgta agaatggatt gctgtacgcc
    11641 acaccgctga tgctggtact gattctggtg gaattgagcg acgtgatttt cgccgtggat
    11701 agcattccgg ctatcttcgc cgtgaccact gacccgttca ttgtgctgac ctcaaacctg
    11761 tttgcgatcc tcggcctgcg tgcgatgtat ttcctgctgg cgggcgtagc agagcgtttc
    11821 tcgatgctca aatatggcct ggcggtgatt ctggtgttta tcggtatcaa gatgctgatt
    11881 gtcgacttct accatattcc aatcgccgtc tcgctgggcg tggtgtttgg cattctggtg
    11941 atgacgttta ttatcaacgc ctgggtgaat tatcggcatg ataagcagcg gggagaatga
    12001 ttcgctgtat attaagccat tcctagagat aactcat
//
LOCUS       DADMKF010000120        11876 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.122, whole genome shotgun sequence.
ACCESSION   DADMKF010000120 DADMKF010000000
VERSION     DADMKF010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11876
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.122"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            207..1760
                     /locus_tag="JNC24_003032"
     CDS             207..1760
                     /locus_tag="JNC24_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000807159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HAY9159721.1"
                     /translation="MSEFARCHYGYIQIPPVTTFRADGPETPEEEKGYWFHAYQPEDL
                     CTIHNPMGDLQDFIALVKDAKKFGIDIIPDYTFNFMGIGGSGKNDLDYPSADIRAKIS
                     KDIEGGIPGYWQGQVLIPFIKDPVTKERKQIHPEDIHLTAKDFEASKDNISKDEWENL
                     HALKEKRLNGIPKTTPKSDQVIMLQNQYVREMRKYGVRGLRYDAAKHSKHEQIERSIT
                     PPLKNYNERLHNTNLFNPKYHKKAVMNYMEYLVTCQLDEQQMSSLLYERDDLSAIDFS
                     LLMKTIKAFSFGGDLLTLASKPGSTISSIPSERRILININHDFPNNGNLFNDFLFNHQ
                     QDEQLAMAYMAALPFSRPLVYWDGQVLKSTTEIKNYDGSTRVGGEAWLNKGCSTYQQL
                     YNEFHALYIDKAGIWSAFEGVFATKNVLAFSRGDSVNINHSPHDGLVIINKGNEEVEG
                     TWPNKLQPGIYKNMGSNSVNIIINNTRKIIPPGKVFTLRGGTLNINIPGRSALLLGKT
                     GEPPNYLYL"
     gene            2096..3073
                     /gene="csiD"
                     /locus_tag="JNC24_003033"
     CDS             2096..3073
                     /gene="csiD"
                     /locus_tag="JNC24_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation induced protein CsiD"
                     /protein_id="HAY9159722.1"
                     /translation="MNALTAVQNNAVDSGQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGI
                     DDVAQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDEQNMQGGNSLLLHLDDWEHLDHYFRHPLARRPMRFAAPP
                     SKNVSKDVFHPVFDVDQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVP
                     VPVGKFLLINNLFWLHGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            3093..4361
                     /gene="lhgO"
                     /locus_tag="JNC24_003034"
     CDS             3093..4361
                     /gene="lhgO"
                     /locus_tag="JNC24_003034"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HAY9159723.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNGIRYDNCGKMLVATSQLEMERM
                     RALWERTAANGIEREWINAVELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSALSKHKNGVVIRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILSSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            4384..5832
                     /gene="gabD"
                     /locus_tag="JNC24_003035"
     CDS             4384..5832
                     /gene="gabD"
                     /locus_tag="JNC24_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HAY9159724.1"
                     /translation="MKLNDSNLFRQQALINGEWLDANNGGIIDVTNPANGDKLGSVPK
                     MGADETRAAIDAANRALPAWRALTAKERANILRNWFNLMMEHQDDLARLMTLEQGKPL
                     AEAKGEISYAASFIEWFAEEGKRIYGDTIPGHQADKRLIVIKQPIGVTAAITPWNFPA
                     AMITRKAGPALAAGCTMVLKPASQTPFSALALAELAIRAGIPAGVFNVVTGSAGAVGN
                     ELTSNPLVRKLSFTGSTEIGRQLMEQCAKDIKKVSLELGGNAPFIVFDDADLDKAVEG
                     ALASKFRNAGQTCVCANRLYVQDGVYDRFAEKLQQAVSKLHIGDGLDKGVTIGPLIDE
                     KAVAKVEEHIADALEKGARVVCGGKADERGGNFFQPTILVDVPANAKVSKEETFGPLA
                     PLFRFKDEADVIAQANDTEFGLAAYFYARDLSRVFRVGEALEYGIVGINTGIISNEVA
                     PFGGIKASGLGREGSKYGIEDYLEIKYMCIGL"
     gene            5846..7126
                     /gene="gabT"
                     /locus_tag="JNC24_003036"
     CDS             5846..7126
                     /gene="gabT"
                     /locus_tag="JNC24_003036"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HAY9159725.1"
                     /translation="MNSNKELMQRRSQAIPRGVGQIHPIFADRAENCRVWDVEGREYL
                     DFAGGIAVLNTGHLHPKVVAAVEAQLKKLSHTCFQVLAYEPYLELCEIMNQKVPGDFA
                     KKTLLVTTGSEAVENAVKIARAATKRSGTIAFSGAYHGRTHYTLALTGKVNPYSAGMG
                     LMPGHVYRALYPCPLHGISEDDAIASIHRIFKNDAAPEDIAAIVIEPVQGEGGFYAAS
                     PAFMQRLRALCDEHGIMLIADEVQSGAGRTGTLFAMEQMGVAPDLTTFAKSIAGGFPL
                     AGVTGRAEVMDAVAPGGLGGTYAGNPIACVAALEVLKVFEHENLLQKANDLGQKLKDG
                     LLAIAEKHPEIGDVRGLGAMIAIELFEDGDHSKPDAKLTAEIVARARDKGLILLSCGP
                     YYNVLRILVPLTIEDAQIRQGLEIISQCFAEAKQ"
     gene            7437..8753
                     /locus_tag="JNC24_003037"
     CDS             7437..8753
                     /locus_tag="JNC24_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HAY9159726.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAG
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDTPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSA
                     LYTASRMLYSLSRRGDAPAVMGKINRSKTPYVAVLLSTGAAFLTVVVNYYAPAKVFKF
                     LIDSSGAIALLVYLVIAVSQLRMRKILRAEGSEIRLRMWLYPWLTWLVIGFITFVLVV
                     MLFRPAQQLEVISTGLLAIGIICTVPIMARWKKLVLWQKTPVHNTR"
     gene            8774..9436
                     /gene="csiR"
                     /locus_tag="JNC24_003038"
     CDS             8774..9436
                     /gene="csiR"
                     /locus_tag="JNC24_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HAY9159727.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLR
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLARDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(9437..9886)
                     /gene="kbp"
                     /locus_tag="JNC24_003039"
     CDS             complement(9437..9886)
                     /gene="kbp"
                     /locus_tag="JNC24_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HAY9159728.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(9970..10128)
                     /locus_tag="JNC24_003040"
     CDS             complement(9970..10128)
                     /locus_tag="JNC24_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HAY9159729.1"
                     /translation="MGFWRIVITIILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
     gene            10311..10610
                     /locus_tag="JNC24_003041"
     CDS             10311..10610
                     /locus_tag="JNC24_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY9159730.1"
                     /translation="MTELAQLQASAEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLIDSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            10620..11144
                     /gene="ygaP"
                     /locus_tag="JNC24_003042"
     CDS             10620..11144
                     /gene="ygaP"
                     /locus_tag="JNC24_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HAY9159731.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKRAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRA"
     gene            complement(11191..11595)
                     /gene="stpA"
                     /locus_tag="JNC24_003043"
     CDS             complement(11191..11595)
                     /gene="stpA"
                     /locus_tag="JNC24_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HAY9159732.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
BASE COUNT         2964 a         2928 c         3062 g         2922 t
ORIGIN      
        1 atgtatataa tattaacgtt acgtcacctt ccggacagca agaaacattt aaaatactaa
       61 tcaatccaga acatctacgg cagacacttg agcgtaaatc tcttactgct gttcagaaat
      121 cacaatgtga aatcatcacc cctaaaaaac ccggcgaagc gattcttcat gcttttaatg
      181 ccacctacca gcaaatcagg gaaaatatgt ctgagtttgc acgttgccat tatgggtata
      241 tacaaatccc tccagtgaca actttccgcg ccgacggacc agaaactccc gaagaagaaa
      301 agggttactg gtttcatgct tatcaacccg aagatctttg taccatccat aatccaatgg
      361 gagatttgca ggattttatt gcattggtta aagatgctaa aaaatttggt atcgatatca
      421 ttcctgatta tacctttaac tttatgggaa ttgggggtag tggtaaaaat gacctggatt
      481 atccctctgc tgatatacga gcgaagatca gtaaagatat agaaggtggt atccctggct
      541 attggcaagg tcaggttttg attccattca ttaaagatcc agtaacaaaa gaacgtaaac
      601 aaatccatcc agaagatata catctcactg caaaagactt cgaagcaagt aaagataaca
      661 tctctaagga tgaatgggaa aacctccatg cattaaaaga aaagcgttta aatggaattc
      721 ctaaaacaac acccaaaagt gaccaggtta ttatgttgca aaatcaatac gttcgtgaaa
      781 tgcgaaaata tggcgtacga ggtttacgtt atgatgcggc aaaacactca aaacatgaac
      841 aaatagaaag atcaataacc ccaccgctta aaaattataa tgagcggtta cacaatacta
      901 acttatttaa cccaaaatat cataaaaaag ccgttatgaa ttacatggaa tatctggtaa
      961 cttgtcagtt ggatgaacaa caaatgtcat cactgcttta tgaaagagat gatttaagcg
     1021 ccattgattt ttcattgctc atgaagacga taaaagcctt ttcattcggg ggagatctcc
     1081 taacccttgc atcaaaaccg ggttccacaa tctcaagcat cccatcagaa agacggatat
     1141 tgattaacat taaccacgat tttcctaaca atggcaatct tttcaatgac tttctattta
     1201 accatcaaca agatgaacaa ttagcaatgg catatatggc cgctctcccg ttcagcagac
     1261 ctttagttta ctgggatggc caggtattaa aatcaacgac tgaaattaaa aattatgatg
     1321 ggtccacgcg tgtcggtggt gaggcgtggc ttaataaagg ttgctctacc tatcagcagc
     1381 tctacaatga attccacgca ttatatatag ataaagcagg aatatggagc gcatttgagg
     1441 gtgtatttgc aactaaaaac gttctagcct ttagtcgtgg ggattctgtg aacattaatc
     1501 actctcctca tgatggacta gttataataa ataaaggaaa cgaagaagtt gaaggtacct
     1561 ggcctaacaa attgcaacct ggaatatata aaaacatggg gagtaatagc gttaacatta
     1621 ttattaataa tacccgaaaa attatccccc ctggtaaagt atttacgctt agaggcggaa
     1681 ctctaaatat caatattcct gggcgtagcg ctcttctttt agggaaaaca ggagaaccgc
     1741 cgaactatct ctatttataa tttaatgtta tatctgcccc gataaaacgg ggcagataat
     1801 atgtttagtt tactaacggt cattttgcag tgaagccatt tactgttttt tatcgaccag
     1861 ataatctgtt ctctaatgtt aactccccct aacctgttgc tttagttatt catttcctgt
     1921 ctcactttgc cttaatactc tacgttaaat gttactaatt tgttgctttt gatcacaata
     1981 agaaaacaat atgtcgcttt tgtgcgcatt tttcagaaat gtagatattt ttagattatg
     2041 gctacgaaat gagcatcgcc atgtcaccct acatctcata agaggatcgc ttctgatgaa
     2101 tgcactgacc gccgtacaaa ataacgctgt cgattcaggc caggactata gcggattcac
     2161 tctcatcccg tcggcgcaat ccccgcgtct gctggaactc accttcaccg aacagacgac
     2221 caaacagttt ctcgagcagg ttgccgaatg gcccgtacag gcgctggagt acaaatcgtt
     2281 tctgcgtttt cgggtaggca aaattcttga cgatctgtgt gcgaatcagc tgcaaccatt
     2341 gctgttgaag accctgctaa accgtgctga aggtgcgctg ttgatcaatg cggtgggtat
     2401 cgatgatgtg gcgcaggcag atgagatggt gaagctggca acggcggtag cgcatctgat
     2461 tggtcgctca aatttcgatg cgatgagcgg tcagtattac gcgcgtttcg tggtgaaaaa
     2521 tgtcgataac tcagacagct atctgcgtca gccgcaccgc gtgatggaac tgcacaacga
     2581 cggcacgtat gtcgaagaga tcaccgatta cgtgctgatg atgaaaatcg atgagcaaaa
     2641 catgcagggt ggaaattcgt tgctgctgca tctcgatgac tgggaacatc tggaccacta
     2701 tttccgccat ccactggcgc gtcgcccgat gcgctttgcc gcgccgccga gcaaaaacgt
     2761 cagcaaagat gttttccatc cagtgttcga cgttgatcaa cagggtcgcc cggtgatgcg
     2821 ctatatcgac cagttcgtcc agccaaaaga ttttgaagaa ggcgtgtggt tgagcgagct
     2881 ttccgatgcc attgaaacca gcaaaggcat tctttctgta cccgttcccg ttggcaaatt
     2941 cctgttgata aacaacctgt tctggctgca cggtcgcgac cgctttactc cgcacccgga
     3001 tctgcgccgt gaactgatgc gtcagcgtgg ctatttcgct tacgccactc accactacca
     3061 gacgcatcag taagcgcaaa ggaattgagc ggatgtatga ttttgtgatt attggcggcg
     3121 gcatcatcgg catgtcgacc gccatgcaac tgattgatgt ttatccggat gcccgcattg
     3181 cgttgctgga aaaagagtcc ggcccggcct gtcaccagac gggccacaac agcggcgtga
     3241 tccatgccgg ggtctattac acgcccggca gcctgaaggc gcagttttgc ctggcgggga
     3301 accgcgccac caaagccttt tgcgatcaaa acggcattcg ctatgacaac tgcggcaaga
     3361 tgctggtcgc cacgtctcag ctcgaaatgg aacggatgcg cgccttatgg gaacgcacag
     3421 cggcgaacgg tatcgagcgc gagtggataa acgccgtgga actgcgcgag cgcgaaccga
     3481 atatcaccgg gcttggtggc atttttgtgc cgtccagcgg cattgtcagc taccgcgaag
     3541 tcacagcggc aatggcaaaa atcttccagg ccagaggcgg cgagattatt tataacgccg
     3601 aagtcagcgc cctcagtaag cataaaaacg gcgtggtgat acgcacccgt cagggcggtg
     3661 aatatgaagc atcgacgctg attagctgtt ccgggctgat ggctgaccgg ctggtgaaaa
     3721 tgctcggcct cgaaccgggc tttatcatct gcccgttccg tggtgagtat ttccgccttg
     3781 cgccggagca taaccagatt gttaaccacc tgatttaccc cattcccgac cccgcgatgc
     3841 catttttggg cgtgcatctc acccgcatga tcgacggcag cgtgaccgtc gggccaaacg
     3901 cggtgctggc tttcaaacgc gaaggctatc gcaagcgcga cttctcattt agtgacacgc
     3961 tggagatttt gagctcgtcg gggattcgcc gggtgctgca aaaccatcta cgctcaggac
     4021 tgggcgagat gaaaaactcg ctgtgcaaaa gcggctacct gcggctggtg caaaagtatt
     4081 gtccccggct ttcgttaagc gatctccagc cctggcccgc cggtgtgcgg gcgcaggcag
     4141 tatcgccgga cggcaagctg attgacgatt ttctgtttgt caccacccca cgcacgatcc
     4201 acacctgcaa tgcgccctcc ccggcagcga catcagcaat tcctattggt gcgcatattg
     4261 tcagcaaggt acaaacgctg ttggcaagcc agagtaaccc cggacgcacg ctgcgagcgg
     4321 cacgtagtgt ggatgcctta cacgccgcat ttaatcaata acctttgaaa acaggacgta
     4381 acaatgaaac ttaacgacag taacttattc cgccagcagg cgttgattaa cggggaatgg
     4441 ctggacgcca acaatggcgg gatcatcgac gtcaccaatc cggcgaacgg cgacaagctg
     4501 ggtagcgtgc cgaaaatggg cgctgatgaa acccgcgccg ctatcgacgc cgccaaccgc
     4561 gccctgcccg cctggcgtgc gctcaccgcc aaagaacgcg ccaacattct gcgcaactgg
     4621 ttcaatttga tgatggagca tcaggacgat ttagcgcgcc tgatgaccct cgaacagggc
     4681 aaaccactgg ccgaagcgaa aggcgaaatc agctacgctg cctcttttat tgagtggttt
     4741 gccgaagaag gcaaacgtat ttatggcgat accattcccg gtcatcaggc cgataagcgc
     4801 ctgattgtta tcaagcagcc gattggcgtt accgcagcta tcacgccgtg gaacttcccg
     4861 gcggcgatga ttacccgtaa agccggtccg gcgctggcgg caggctgcac catggtgctg
     4921 aagcccgcca gtcagacacc gttctctgcg ctggcgctgg cggagctggc gattcgcgcg
     4981 ggcattccgg ctggcgtgtt taacgtagtc accggttcgg caggcgcggt cggtaatgaa
     5041 ctgaccagca acccgctggt acgcaaactg tcgtttaccg gttcgaccga aattggccgc
     5101 cagttaatgg aacagtgcgc gaaagacatc aaaaaagtgt cgctggagct gggcggcaac
     5161 gcgccgttta tcgtctttga cgatgccgat ctcgataaag ccgtggaagg cgcgctggcc
     5221 tcgaaattcc gcaacgccgg gcaaacctgc gtctgcgcca accgtttata cgtgcaggac
     5281 ggcgtgtatg accgctttgc cgaaaaattg cagcaggcgg tgagcaaact gcacatcggc
     5341 gacgggctgg ataaaggcgt caccatcggg ccgctgatcg atgaaaaagc ggtagcaaaa
     5401 gtggaagagc atattgccga tgcgctggag aaaggtgcgc gtgtggtttg cggcggtaaa
     5461 gcggatgaac gcggcggcaa cttcttccag ccgaccattc tggtggacgt gccggccaac
     5521 gccaaagtgt cgaaagaaga gacgttcggc cccctcgccc cgctgttccg ctttaaagat
     5581 gaagccgatg tgattgcgca agccaatgac accgagtttg gccttgccgc ctatttctac
     5641 gcccgtgatt taagccgcgt cttccgcgtg ggcgaagcgc tggagtacgg catcgtcggc
     5701 atcaataccg gcattatttc caatgaagtg gccccgttcg gcggcatcaa agcctcgggt
     5761 ctgggtcgtg aaggttcgaa gtatggcatc gaagattact tagaaatcaa atatatgtgc
     5821 atcggtcttt aactggagaa tgcgaatgaa cagcaataaa gagttaatgc agcgccgcag
     5881 tcaggcgatt ccccgtggcg ttgggcaaat tcacccgatt ttcgctgacc gcgcggaaaa
     5941 ctgccgggtg tgggacgttg aaggccgtga gtatcttgat ttcgcgggcg ggattgcggt
     6001 gctcaatacc gggcacctgc atccgaaggt ggtggccgca gtggaagcgc agttgaaaaa
     6061 actgtcgcac acctgcttcc aggtgctggc ttacgagccg tatctggagc tgtgcgagat
     6121 tatgaatcag aaggtgccgg gcgatttcgc caagaaaacg ctgctggtta cgaccggttc
     6181 cgaagcggtg gaaaacgcgg taaaaatcgc ccgcgccgcc accaaacgta gcggcaccat
     6241 cgcttttagc ggcgcgtatc acgggcgcac gcattacacg ctggcgctga ccggcaaggt
     6301 gaatccgtac tctgcgggca tggggctgat gccgggtcat gtttatcgcg cgctttatcc
     6361 ttgcccgctg cacggcataa gcgaggatga cgctatcgcc agcatccacc ggatcttcaa
     6421 aaatgatgcc gcgccggaag atatcgccgc catcgtgatt gagccggttc agggcgaagg
     6481 cggtttctac gccgcgtcgc cagcctttat gcagcgttta cgcgctctgt gtgacgagca
     6541 cgggatcatg ctgattgccg atgaagtgca gagcggcgcg gggcgtaccg gcacgctgtt
     6601 tgcgatggag cagatgggcg tggcgccgga tcttaccacc tttgcgaaat cgatcgcagg
     6661 cggtttcccg ctggcgggcg tcaccgggcg cgcggaagtg atggacgctg ttgcaccggg
     6721 cggtctgggc ggcacctatg ccggtaaccc gattgcctgc gtggctgcgc tggaagtgtt
     6781 gaaggtgttc gagcatgaaa atctgctgca aaaagccaac gatctggggc agaagctaaa
     6841 agacggattg ctggcgatag ccgaaaaaca cccggagatc ggcgacgtac gcgggctggg
     6901 agcgatgatt gccatcgagc tgtttgaaga tggcgatcac agcaaaccgg acgcaaaact
     6961 caccgccgag atcgtggcac gcgcccgcga taagggcctg attcttctct cctgcggccc
     7021 gtattacaac gtgctgcgca tccttgtacc gctcaccatt gaagacgctc agatccgtca
     7081 gggtctggag atcatcagcc agtgttttgc tgaggcgaag cagtagctat gtccgtttgt
     7141 aagagttaac cgttgctaag ggaatgataa cgatgcagta aaaaataata acgatgaggc
     7201 gaacgacaaa gttaaaaacg actgcggaca gtcccctcgc ccctctggag agagggttag
     7261 ggtgaggggt aaaacacgcg gctaaaccct cccctggccc tctccctaag ggagagggag
     7321 aaaaccctcc cgctttcgtt tttaagattt tgttgaccgt ctcaattaaa cgcgtgaccc
     7381 ggcgcgggaa atgtcggggc gctctcccaa gtgacacact ttcgagagga ttcaggatgg
     7441 ggcaatcatc gcaaccacat gagttaggcg gcgggctgaa atcacgccac gtcaccatgt
     7501 tgtctattgc cggtgttatc ggcgcaagtc tgtttgtcgg ttccagcgtc gccatcgccg
     7561 aagcgggccc ggcggtatta ctggcctatc tgttcgccgg gctactggtg gttatgatta
     7621 tgcggatgtt ggcggaaatg gcggttgcca cgcccgatac cggttcgttt tccacctatg
     7681 ccgataaagc cattggtcgc tgggcgggct ataccatcgg ctggttgtac tggtggtttt
     7741 gggtactggt tatcccgctg gaagccaata tcgccgctat gatcctgcac tcgtgggttc
     7801 caggcattcc catctggtta ttttccctcg tcattaccct tgccttaact ggcagtaatt
     7861 tattaagcgt taaaaactac ggcgaatttg agttctggct ggcgctgtgc aaagtcatcg
     7921 ctatcctggc ctttattttc cttggtgcag tcgcaattag cggtttttac ccttatgccg
     7981 aagtgagcgg gatctcaaga ttgtgggata gcggcggctt tatgcccaac ggtttcggtg
     8041 cggtattaag cgcgatgttg atcaccatgt tctcgtttat gggcgcagaa attgtcacca
     8101 ttgccgccgc ggaatccgac acgccggaaa aacatattgt ccgcgccacc aactcggtta
     8161 tctggcgtat ttctatcttc tatttgtgct ctatttttgt cgtcgtggca ttaataccgt
     8221 ggaatatgcc ggggctgaaa gccgttggat cttatcgctc ggccctgtat accgcctcaa
     8281 ggatgctcta ctccttaagc cgtcgcggtg atgctcccgc ggtaatgggt aaaatcaacc
     8341 gcagtaaaac cccgtacgtg gcggtgttac tctccaccgg cgcggcattc ttaacggtgg
     8401 tggtgaacta ttacgcgcct gcgaaggtat ttaaatttct gatcgacagc tccggcgcta
     8461 tcgccctgct ggtttattta gtcatcgccg tttcacagtt gcggatgcgc aaaattctgc
     8521 gagcggaagg aagcgaaatt cgcttgcgaa tgtggcttta tccctggctc acctggctgg
     8581 tcatcggctt tattaccttt gtgttggtag tgatgctatt ccgtccggcg caacagttag
     8641 aagtgatctc caccggctta ttagcgatag ggattatctg taccgtgcca attatggctc
     8701 gctggaaaaa gctggtattg tggcaaaaaa cacccgttca taatacgcgc tgataatgat
     8761 caggagtcac accatgacca ttacgtctct ggatggctat cgctggctga agaacgatat
     8821 tattcgcggt aattttcaac cggatgaaaa attacggatg agtttgctga catcgcgtta
     8881 tgcacttggc gttgggccgt tgcgggaagc actttctcaa ctggtggcgg aacggctggt
     8941 cacggtggtg aatcaaaaag ggtatcgggt ggcgtctatg tcagagcagg agctgctcga
     9001 tattttcgat gcccgcgcca atatggaagc gatgttagtg agtctggcga ttgcccgcgg
     9061 tggcgatgag tgggaggcag acgttctcgc aaaagcgcat ctgctcagta agctggaagc
     9121 ctgtgacgcc agcgagaaaa tgcttgatga gtgggatctg cgtcatcagg cgtttcatac
     9181 agcaattgtg gcgggctgtg gttctcacta tttgctgcaa atgcgtgaac ggttgtttga
     9241 tctggcggcg cgctatcgat ttatctggct gcggcgaacg gtgctttcgg tagaaatgct
     9301 ggaggataaa cacgatcagc accagaccct gactgcggcg gtactggcgc gagataccgc
     9361 gcgcgccagt gagttaatgc gccagcattt actgacgcca attcccatta tccagcaggc
     9421 gatggctggc aattaactac tcttccggaa tacgcaacac ttgccccgga taaattttat
     9481 ccgggctttt tagcatcggt ttattcgctt cgaagatttt attgtacaga ttagcgttac
     9541 cgtagacctg tttggaaatg gcactcagag tgtcgccaga cttaacggta taaaactggc
     9601 tggcagtggc tggtgtcgcc gttttcacct gatcatcaac actggcaata ccggaaatat
     9661 tccccaccgc aacaaggatt ttctccttcg cctcctgact caggccgtca ccagtgaccg
     9721 tcgctttgcc gtcggcaatt tgaatgttca ctttatcagc atcaggtata ccggttttgc
     9781 tcagatgctc ctgcaccttc ttcgcctgat cgtctttatc gtgctgacct gtaaccgcgt
     9841 cccagagttt ttctccggca tctttcacaa aattgaacag acccatagct acctcattcg
     9901 ttaacggaaa cactcagaaa gtgtagcaga ggaacgcggc gcgttaacgc cgcgggggtg
     9961 agcacaggtt taatcgcggg tttgcaccca gaacgcgtga atcaaaccgg ggatataccc
    10021 aagcagtgtc agcagaatat taataatgaa cgcccaaccg aaccctttac cgagcagcac
    10081 gccgagcggc ggtagaatga tggtgatgac gattctccag aaacccatat gtactcccta
    10141 taagaaaatt actcattgtt taaaaagaga ttttatctct taagcgtagt aattttagcg
    10201 gagcctgcca gtttcagctt acgcttttac tctcatttac gctgctgagg ctggtatatg
    10261 ttgcattttt agtttagagc ttacttaatt tagaaaatac ttaaataaat atgactgaac
    10321 tcgcgcaatt acaggccagt gccgaacagg cagcggcctt attgaaagca atgagccatc
    10381 ctaaacgatt gctgattctg tgcatgctta gcggttcccc cggcaccagc gcgggagagc
    10441 tgacgcgcat taccggactg agtgcctctg cgacatcaca gcatctcgct cgtatgcggg
    10501 acgaagggct tatcgacagc caacgggatg cccaacgcat tctatattcc attaaaaatg
    10561 aggcggtaaa tgccattatc gccaccctga aaaatgtcta ttgtccgtaa ggagtcacta
    10621 tggctttgac aaccatttcg ccgcatgatg cacaagaatt aatcgcacgc ggcgcaaagt
    10681 taatcgatat tcgtgacgct gatgaatatc ttcgtgaaca tattcctgaa gcagatctgg
    10741 ctccattatc cgtgctggaa cagtcaggtc ttccacctaa attacgtcgc gagcaaatta
    10801 tattccactg ccaggcaggt aaacgcacca gtaataacgc cgataaatta gcagcgattg
    10861 ccgcccccgc agaaatattt ttactcgaag atgggattga tggctggaaa agagctggat
    10921 tgccagtagc ggtaaataaa tctcaaccct tgccgttaat gcgccaggtg cagatcgctg
    10981 cgggtggttt aatattaatc ggcgttgtac tgggttatac cgtaaatagc ggtttcttct
    11041 tattaagtgg ctttgttggt gccgggttac tgtttgcagg aatcagcggt ttttgtggaa
    11101 tggcaaggtt gttagataag atgccgtgga accaacgagc ttgagaagcg acgccggacg
    11161 cgccctagca gcgacatccg gcctcagtaa ttagatcagg aaatcgtcga gagatttacc
    11221 ttctgccagc gcctgagcaa ttggcttcgg tgtacggccc tgaccggtcc aggttttagt
    11281 ttcaccgtta acatcggtga atttatattt cgccggacgc ggctggcgtt ttttaccagc
    11341 gcgtggtgca gcagcagagc tattacccaa taactcttcc gggttaattc cgtcagcctt
    11401 catcagctcc agccaggtgc taattttttc ctgacgctca gccagttcac gctgctgctg
    11461 ttcttcttct tcacgtcttt ctttagtgac aaccctgaat ttttcgagca tttcttcaag
    11521 aacgtcaatg gagaattcgc gagccatcgc acggagggtg cgaatgttat ttaaactttg
    11581 taacattacg gacataataa agaaaacctt ttaacgccaa aacaaaaaag tatttcgtcg
    11641 cgtagtgtaa gcgatcactt attatatttc cacaaaattt aatctattta cagtcttgcg
    11701 cgagattatt tcagctgtta ttttctgaaa ctctcagaat aatttcaaga ttatatatta
    11761 gccattttga tgagtaattt agattattgg tgaacagaac ggcgctctct gctcgctaat
    11821 tccgcaagcc tgacaaaacc gaagtgaata aaaatgcaat ataacagaat aaaaga
//
LOCUS       DADMKF010000121        11864 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.123, whole genome shotgun sequence.
ACCESSION   DADMKF010000121 DADMKF010000000
VERSION     DADMKF010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11864
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.123"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(105..368)
                     /gene="cedA"
                     /locus_tag="JNC24_003044"
     CDS             complement(105..368)
                     /gene="cedA"
                     /locus_tag="JNC24_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HAY9159733.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
     gene            551..2812
                     /gene="katE"
                     /locus_tag="JNC24_003045"
     CDS             551..2812
                     /gene="katE"
                     /locus_tag="JNC24_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HAY9159734.1"
                     /translation="MSQHNEKNLHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGNRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKALK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSADGSFMDELLTLMAAHR
                     VWSRIPKIDKIPA"
     gene            complement(3070..3828)
                     /gene="chbG"
                     /locus_tag="JNC24_003046"
     CDS             complement(3070..3828)
                     /gene="chbG"
                     /locus_tag="JNC24_003046"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HAY9159735.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMYFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRIDRQPLSNSG
                     DLPANLRSSQGFSSAFYGEEISEALFLQVLDDASHRGDRSLEVMCHPAFIDNTIRQSA
                     YCFPRLTELDVLTSASLKGAIAQRGYRLGSYRDV"
     gene            complement(3841..5193)
                     /locus_tag="JNC24_003047"
     CDS             complement(3841..5193)
                     /locus_tag="JNC24_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAY9159736.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEDG
                     KEKLDIIFELCQRMIDNAGVPMKLYKTLDRREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIHDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKASGVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCKLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            complement(5298..6137)
                     /gene="chbR"
                     /locus_tag="JNC24_003048"
     CDS             complement(5298..6137)
                     /gene="chbR"
                     /locus_tag="JNC24_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
                     /protein_id="HAY9159737.1"
                     /translation="MQPVINAPEIATAREQQLFNGKNFHVFIYNKTESISGLHQHDYY
                     EFTLVLTGRYFQEINGKRVLLERGDFVFIPLGSHHQSFYEFGATRILNVGISKRFFEQ
                     HYLPLLPYCFVASQVYRTNNAFLTYVETVISSLNFRETGLEEFVEMVTFYVINRLRHY
                     REEQVIDDVPQWLKSTVEKMHDKEQFSESALENMVTLSAKSQEYLTRATQRYYGKTPM
                     QIINEIRINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTPKSYRKKLTE
                     FNQ"
     gene            complement(6148..6498)
                     /gene="chbA"
                     /locus_tag="JNC24_003049"
     CDS             complement(6148..6498)
                     /gene="chbA"
                     /locus_tag="JNC24_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HAY9159738.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(6549..7907)
                     /gene="chbC"
                     /locus_tag="JNC24_003050"
     CDS             complement(6549..7907)
                     /gene="chbC"
                     /locus_tag="JNC24_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
                     /protein_id="HAY9159739.1"
                     /translation="MSNVIASLEKVLLPFAVKIGKQPHVNAIKNGFIRLMPLTLAGAM
                     FVLINNVFLSFGEGAFFYSLGIRLDASTIETLNGLKGIGGNVYNGTLGIMSLMAPFFI
                     GMALAEERKVDALAAGLLSVAAFMTVTPYSVGEAYVVGANWLGGANIISGIIIGLVVA
                     EMFTFIVRRNWVIKLPDSVPASVSRSFSALIPGFIILSVMGIIAWALNTWGTNFHQII
                     MDTISTPLASLGSVVGWAYVIFVPLLWFFGIHGALALTALDNGIMTPWALENIATYQQ
                     YGSVEAALAAGKTFHIWAKPMLDSFIFLGGSGATLGLILAIFIASRRADYRQVAKLAL
                     PSGIFQINEPILFGLPIIMNPVMFIPFVLVQPILAAITLAAYYMGIIPPVTNIAPWTM
                     PTGLGAFFNTNGSVAALLVALFNLGIATLIYLPFVVVANKAQNAIDKEEREEDIANAL
                     KF"
     gene            complement(7992..8312)
                     /gene="chbB"
                     /locus_tag="JNC24_003051"
     CDS             complement(7992..8312)
                     /gene="chbB"
                     /locus_tag="JNC24_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HAY9159740.1"
                     /translation="MEKKHIYLFCSAGMSTSLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(8611..8949)
                     /gene="osmE"
                     /locus_tag="JNC24_003052"
     CDS             complement(8611..8949)
                     /gene="osmE"
                     /locus_tag="JNC24_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HAY9159741.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            9151..9978
                     /gene="nadE"
                     /locus_tag="JNC24_003053"
     CDS             9151..9978
                     /gene="nadE"
                     /locus_tag="JNC24_003053"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HAY9159742.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSIDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRLETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLAALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNVPEQVARTIENWYLKTEHKRRPPITVFDDFWKK"
     gene            10208..11095
                     /gene="cho"
                     /locus_tag="JNC24_003054"
     CDS             10208..11095
                     /gene="cho"
                     /locus_tag="JNC24_003054"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HAY9159743.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLRSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQTIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(11055..11630)
                     /gene="ves"
                     /locus_tag="JNC24_003055"
     CDS             complement(11055..11630)
                     /gene="ves"
                     /locus_tag="JNC24_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HAY9159744.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMLLESADRFNHTLKPLQPFAFAADQVVKAKLTEGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
BASE COUNT         3137 a         3044 c         2711 g         2972 t
ORIGIN      
        1 cgtcaaaggc agctttttgt tacaggtggt ttgaatgaat gtagcaacga aatgcagaat
       61 ttcaggtcat gtaactcccg gcaaaaccgg gaagtatgta atccttactc agtcacttcc
      121 ccttcctggc ggatctgatt tgcccaacgt tgggcagatt caggcacagt aaacgccggt
      181 gagcgcagaa atgactctcc catcagtaca aacgcaacat atttgccacg cagcatccag
      241 acatcacgaa acgaatccat ctttatcgca tgttctggcg gcgcgggttc cgtgcgtggg
      301 acatagctaa taatctggcg gttttgctgg cggagcggtt tcttcattac tggcttcact
      361 aaacgcatat taaaaattag aaaaactgta gtttagccga tttagcccct gtacgtcccg
      421 ctttgcgtgt atttcataac accgtttcca gaatagtctc cgaagcggga tctggctggt
      481 ggtctatagt tagagagttt tttgaccaaa acagcggccc tttcagtaat aaattaagga
      541 gacgagttca atgtcgcaac ataacgaaaa gaacctacat cagcaccagt caccactaca
      601 cgattccagc gaagcgaaac cggggatgga ctcactggca cctgaggacg gatctcatcg
      661 tccagcggct gaaccaacac cgccaggtgc acaacctacc gcccctggga gcctgaaagc
      721 ccctgataca cgtaacgaaa aacttaattc tctggaagac gtacgcaaag gcagtgaaaa
      781 ttatgcgctg accactaatc agggcgtgcg catcgccgat gatcaaaact cactgcgtgc
      841 cggtaaccgt ggtccaacgc tgctggaaga ttttattctg cgcgagaaaa tcacccactt
      901 tgaccatgag cgcatcccgg aacgtattgt tcatgcacgc ggatcagcag ctcacggtta
      961 tttccagcca tataaaagct taagcgatat caccaaagcg gatttcctct cagatccgaa
     1021 caaaatcacc ccagtatttg ttaggttctc taccgttcag ggtggcgctg gctctgcaga
     1081 taccgtgcgt gatatccgtg gttttgccac caagttctat actgaagagg gtatttttga
     1141 cctcgttggc aataacacgc caatcttctt tatccaggat gcgcataaat tccccgattt
     1201 tgttcatgcg gtaaaaccag aaccgcactg ggcaattcca caagggcaaa gtgcccacga
     1261 tactttctgg gattatgttt ctctgcaacc tgaaactctg cacaacgtga tgtgggcgat
     1321 gtcggatcgc ggcatccccc gcagttaccg caccatggaa ggcttcggta ttcacacctt
     1381 ccgcctgatt aatgccgaag ggaaggcaac gtttgtacgt ttccactgga aaccactggc
     1441 aggtaaagcc tcactcgttt gggatgaagc acaaaaactc accggacgtg acccggactt
     1501 ccaccgccgc gagttgtggg aagccattga agcaggcgat tttccggaat acgaactggg
     1561 cttccagttg attcctgaag aagatgaatt caagttcgac ttcgatcttc tcgatccaac
     1621 caaactgatc ccggaagaac tggtgcccgt tcagcgtgtc ggcaaaatgg tgctcaatcg
     1681 caatccggat aacttctttg ccgaaaacga acaagcagca ttccatccag gtcatattgt
     1741 tcctggtctg gacttcacca acgatccgct gttgcaggga cgtttgttct cctataccga
     1801 tacacaaatc agtcgtcttg gtgggccgaa tttccatgag attccgatta accgtccgac
     1861 ctgcccttac cacaacttcc agcgtgatgg catgcatcgt atggggatcg acactaaccc
     1921 ggcgaattac gaaccgaact cgatcaacga taactggccg cgcgaaacac cgccgggacc
     1981 gaaacgcggc ggttttgaat catatcagga acgcgtggaa ggaaataaag ttcgtgagcg
     2041 cagtccatcg tttggcgaat attattccca tccgcgtctg ttctggctaa gtcagacgcc
     2101 attcgagcag cgccatattg tcgatggttt cagttttgag ttaagcaaag tcgttcgtcc
     2161 gtatattcgt gagcgcgttg ttgaccagct ggcgcatatt gatctcactc tggcccaggc
     2221 ggtggcgaaa aatctcggta tcgaactgac tgacgaccag ctgaatatca ccccacctcc
     2281 ggacgtcaac ggtctgaaaa aggatccatc cttaagtttg tacgccattc ctgacggtga
     2341 tgtgaaaggt cgcgtggtag cgattttact taatgatgaa gtgagatcgg cagaccttct
     2401 ggccattctc aaggccctga aggccaaagg cgttcatgcc aaactgctct actcccgaat
     2461 gggtgaagtg actgcggatg acggtacggt gttgcctata gccgctacct ttgccggtgc
     2521 accttcgctg acggtcgatg cggtcattgt cccttgcggc aatatcgcgg atatcgctga
     2581 caacggcgat gccaactact acctgatgga agcctacaaa caccttaaac cgattgcgct
     2641 ggcgggagac gcgcgcaagt ttaaagcaac aatcaaggtc gctgaccagg gtgaagaagg
     2701 gattgtggaa gctgacagcg ctgacggtag ttttatggat gaactgctaa cgctgatggc
     2761 agcacaccgc gtgtggtcac gcattcctaa gattgacaaa attcctgcct gatgggaggc
     2821 ggcgcaattg cgccgcctca aatgatttac atagtgcgct ttgtttatgc cggatgcggc
     2881 gtgaacgcct tatccggcct acaaaactgt gcaaattcaa tatattgcag gaaacacgta
     2941 ggcctgataa gcgaagcgca tcaggcagtt ttgcgtttgt cagcagtctc aagcggcgca
     3001 gttacgccgc ctttgtagga attaatcgcc ggatgcaagg ttcacgccgc atctggcaaa
     3061 catcctcact tacacatccc gataactccc caaccgataa ccacgctgag cgatagcacc
     3121 tttcaacgac gctgatgtca acacatccag ctccgttaag cgtgggaaac agtaagcact
     3181 ctgacggata gtattatcga taaacgccgg atgacacatc acctccagcg aacgatcacc
     3241 ccgatggctt gcgtcatcca gcacttgcag gaacagtgcc tcgctaatct cttcaccata
     3301 aaacgcgcta ctgaatccct gagaactgcg cagattagcc ggtaaatcac cagagttaga
     3361 tagcggctga cggtcgatgc gcaacgcaat cccctcttca gccgcaaacc tggcaacaat
     3421 cgggaaaatc tgcgggaaca tatgcacatg atgatggcta tcaagatgcg taggtttgcg
     3481 tccaaatagc tcaatgaaac gcaaatactg actggcaagc tcctgagtaa tttcttccag
     3541 cggtaaagca tcttcttctg ccaactgcca gatccatttt cccagcacac catcgcgggt
     3601 taaccccggc atagctgtca gtggcttgcc catagtaagg acaaagtaca tccctatggc
     3661 cagactcggt tcatcacgac tcaactgcac cgcatggtca atagcctgcc cattcaccag
     3721 cgccgtcgtc gatgtgacaa tcccattgcg acaggcctcg ataatgccgt agttctgacc
     3781 tttgcttaag ccaaaatcat cggcattaac aatcagtaag cgttccataa tcagcctcgg
     3841 ttaatgtgct tttttaagct ctgcgatgca gtcggcaaaa tttggcagcc atttctcgtg
     3901 cgccagaatc atctcgcgtg ccagcagctc agcatcgcga tcggaatgca ccaacggact
     3961 aaggtttagc gccagtaaca catcgttaaa ttctccgcta agtgcggcgt tgctggcagc
     4021 aatctcgaag cctttaatgg tgtgaatcag ccccatcact ttatcatcga aatgcgtaat
     4081 gcgcggatgt ggcgtcgcgc catcgcgccc cagcttacag gtcatttcta ccgcccagtc
     4141 tgccggaata ttatcaatat gcccatgatg cgggatatta acgtaatgtt cagcttgctt
     4201 gtcgttgtag atagcgttga tcacttcgca cgctgcatca gagtaataag ccccaccgcg
     4261 ctgttccagt tctttcggct taactttcaa ctccgggttt ttatacagct caaaaagttg
     4321 tttctcgact ttctgtacta cctgcgctcg tgcgccgcct ttgtagtatt cgcccatttc
     4381 aatagccagc atctcttttt gcttgaagta ataaagcaaa taagaacacg gcaacagatt
     4441 cagagaacga attaagcctt cgctaaatgg cagatcgaaa atatttttaa cgccagatgc
     4501 ttttaactgc ccggatgcca caccatcaag caattcggca aagcgcgact tgccatttac
     4561 cagcacatcc ttaatgaaca ccatatggtt gaggccgaac aaatcgatag ataaatcatc
     4621 gctgtctttc agcatcagaa catcgtgaat aaacatcttc atgccgatcg gaatattaca
     4681 cacgccgata aagcgtttaa atccggtatg acgataaacg gcttcagtaa ccattcccgc
     4741 cgggttagtg aagttaatca cccatgcatt cggacaaagt tcttcgacat cttttacgat
     4801 gtcaaaaatc accggaatgg tacgcagacc tttaaacaga ccgcccgcgc cgttggtttc
     4861 ctggccaaga taaccatgac ttaatggaat acgttcatcc agttcgcgcg ccggtaattg
     4921 gcctacgcgc agttgggtag taacgaaatc agcatctttc aatgcttcgc ggcgatccag
     4981 cgttttataa agcttcatcg ggacgccagc gttatcaatc atccgttggc agagttcaaa
     5041 aatgatatcc agtttctctt taccatcttc gacatccacc agccataatt cgctgaccgg
     5101 caattcgtga taacgcttaa taaatccttc cagtaactcc ggggtatagc tgctcccgcc
     5161 accaatagtg acgactttta atttctggct cataatttct cccttcagta cagaatactg
     5221 atatctggca tatctgcccc cccggacata aataatccag caacaggaca gatatgtgaa
     5281 ttgtcaggta taacgactta ctgattaaat tcagtcaatt tcttacgata gctcttaggc
     5341 gtaaaggacg ttaatttctt aaacgtttta ataaacaaac tcgggctact ataaccggcc
     5401 tcaaacgcaa tatccgtcac ggaatagttg gtcatttcca gttgtttttt ggcaaaatta
     5461 atacggatct cattaataat ctgcattggt gttttgccat aatatcgttg agtcgctcgc
     5521 gtcaaatatt cctgtgattt ggctgacaac gtcaccatat tctccagcgc cgattcacta
     5581 aactgctctt tatcatgcat cttttctacc gtacttttca gccactgcgg tacatcatca
     5641 atcacctgtt cttcgcggta atgacgtaaa cggttaatga cataaaaagt aaccatctca
     5701 acaaactctt ccagccctgt ttcgcggaaa ttcaatgaag aaatcactgt ttccacatag
     5761 gtgagaaaag cgttattggt ccggtatacc tgcgaagcga caaagcaata aggcaacaac
     5821 ggcaggtaat gctgctcaaa aaagcgttta ctgatcccaa cgttcaatat acgcgtggca
     5881 ccaaactcat aaaaactttg atggtgcgaa cctaacggaa taaaaacaaa atcgccccgt
     5941 tccagtaaca cgcgcttacc gttaatctcc tggaaataac gcccggttaa taccagagta
     6001 aattcataat agtcgtgctg atgcagcccg ctgatactct cagttttgtt atagataaac
     6061 acatggaagt ttttgccatt aaacaactgc tgttctcggg cagtggcaat ttccggcgcg
     6121 ttaatcactg gctgcatcat cgactcctta tgccttcagt ttttcatgaa gctcaattaa
     6181 ttcagtaatc agttcacgcg caagcatgga cgtcattaaa tgatcctgag cgtggaccag
     6241 caccagactc actttcatct taccttcgcc cgcatcgcct tcaatcagtt tcgtctgtac
     6301 cagatgcgct tcattcaatg ccatccgtga ctgatccatc atggcttttg ctgcagcaaa
     6361 atcgccctgc ttcgcctgtt tcagtgccgc atacgccagg ctgcgcgctt gtccggagtt
     6421 gatgataagc cccatcacca cttcttccag ctcttcagct tccgtttgcg tatcgggaat
     6481 gttatcgaga tccatcatac atcgttcctc tctattctta ccggcacgat tacccgtacc
     6541 ggcatcgatt aaaatttcag ggcgttagcg atatcttctt cgcgctcttc tttatcaatc
     6601 gcgttttgtg ctttgttagc caccacaaca aagggcagat aaattaacgt tgcgatacca
     6661 aggttgaaga gtgcaaccag caatgcggcg acgctaccgt tggtgttaaa gaaggctccc
     6721 agaccggttg gcatggtcca cggtgcaata ttggtcaccg gaggaatgat gcccatgtag
     6781 tacgctgcga gggtgattgc cgccagaatc ggttgtacca gtacaaacgg gataaacatc
     6841 accgggttca tgataattgg cagaccaaac agaatcggtt cgttaatctg gaagatgccg
     6901 gacggcagcg ccagttttgc cacctgacga taatcagcac ggcgagaggc gataaagata
     6961 gccaggatca ggcctaaagt cgcaccactg cccccaagga aaataaagga gtccagcatc
     7021 ggcttggccc agatatggaa ggtcttaccg gctgccagcg ccgcttcgac ggaaccatat
     7081 tgctgatagg tcgcgatatt ttccagcgcc cacggcgtca taatgccgtt gtccagtgcg
     7141 gtcagcgcca gcgcgccatg aataccgaag aaccagagca gtggaacaaa gatcacatag
     7201 gcccagccca ccacgctacc caacgatgcc agtggggttg agatggtatc cataatgatc
     7261 tgatggaagt tggtgcccca ggtattcaac gcccaggcaa taatccccat cacggaaaga
     7321 ataataaagc cgggaattaa tgccgagaag gaacgcgata ctgaagcagg tacgctatcg
     7381 ggcaatttaa tgacccaatt gcggcggaca ataaaggtaa acatttctgc caccaccagg
     7441 ccaataataa tcccggagat gatattcgct ccacctaacc agtttgcgcc aaccacatag
     7501 gcctcaccga cactatatgg ggtgacggtc ataaatgctg caacggataa caacccagcc
     7561 gccagcgcat cgactttacg ctcttctgcc agcgccatgc caataaagaa cggtgccatt
     7621 aaagacatta tccctaatgt tccgttatat acgttgccgc caataccttt caggccatta
     7681 agtgtttcaa tggttgaggc gtcgaggcga atacctaagg aataaaaaaa cgccccctcc
     7741 ccaaagctta gaaaaacgtt gttaattaat acaaacatgg cccccgcaag ggttaacggc
     7801 attaagcgaa taaagccatt tttgattgca ttaacgtgtg gctgctttcc tattttaact
     7861 gcaaaaggga ggagtacctt ttcaagcgat gcaataacat tactcataga aaaataccct
     7921 taaaaaccgc aatttaaata ttgcggtatt gatttatgaa ataactcttt gacgggaaaa
     7981 tttaaaataa attaatttgc tgcggctttt ttaatcgctg caaccgcagc cttaagcacg
     8041 cctaaaccat cgactttgcc ataaagcagc gagtcaatta cttcaaccgg tttgttgggt
     8101 aacaaacgct ggatttcggg caacatataa gcaatctgcg gccctaataa cacgacatcg
     8161 gcattctgac ctttttcacc agccagtgtt tccggaaatg cttcaataat gaccggaact
     8221 tcatattttt ctgcctgtgc gcgcattttt gataccagta aagaggtaga catgcccgca
     8281 gaacaaaaca gataaatgtg tttcttttcc ataaaactgc cctcatcgac gattatctgt
     8341 cagccagaca ctccgcaagc cttaacctgc ttccatgctc tgggtaactt gcgaaaccaa
     8401 catgactttt tttgtggctg aaaggagtat acggtataga cctgagcaat agtatttctt
     8461 tgaccttata aattgtttct ccttgacctg tggttatgac cctcttcaca tttcgggcaa
     8521 ataattcgcg aagataatta agaactcagg ccaaaaaaaa ccggcgcaat ggccggtttc
     8581 cgttgttact caagctttca gacgaattga ttacttcgca gcctgtggat cagtgtcgta
     8641 ttcagcacag gtctgataac cggagttgat gacatgtccg gtatcatcta acgcgacaaa
     8701 gtaggtttct gctttaccat cacgttgacc caggatgtag gtctggcaag taccacgagc
     8761 atggatcatg ctcacttcag acgaaggttt acccgcaatt tgtgcaacct gcgcccggct
     8821 catgcctttt ttgacgtctt tcaccacagg ctgtacaaac tggtctttag tacgatcata
     8881 agccgtacaa cccgccagca tggttaatac cgccgctgca ctcagaattc ctgccatatt
     8941 cttgttcata ttccgtcctc ttgtttatca gcgtgttaga taagcctgga atacattggg
     9001 cgctttttca agcccgcgaa cgaaacggct ccgctttcag aggattcctg tatgacgttt
     9061 taaccaccat tcagcccgct gtcgcttgtc gtttcagtgg caacgggtta gctttaagga
     9121 agttttgtct tttctgtctg gaggggttca atgacattgc aacaacaaat aataaaggcg
     9181 ctgggcgcaa aaccgcagat taatgctgaa gaggaaattc gtcgtagtat cgattttctg
     9241 aaaagctacc tgcaaactta tccgttcatt aaatcactgg tgctcgggat cagcggcggt
     9301 caggactcca cgcttgccgg aaagctgtgc cagatggcga ttaatgagct gcgcctggaa
     9361 accggcaacg aatcactgca atttattgcc gtacgcctgc cctatggtgt tcaggccgac
     9421 gaacaagatt gccaggatgc cattgccttt attcaaccgg atcgcgtatt aaccgttaat
     9481 atcaagggcg cggtattggc cagcgaacag gcattgcggg aagcaggcat tgaactgagc
     9541 gattttgtcc gtggcaatga aaaagcgcgt gagcggatga aagcacaata tagcattgcg
     9601 ggtatgacca gcggtgtcgt ggtgggcacc gatcatgcag cagaagccat taccggattc
     9661 ttcactaaat atggtgacgg cggtacagac attaatccgc tgtatcgtct caacaaacgt
     9721 cagggtaaac agttactggc ggcattaggt tgcccggaac acctttataa gaaagcgcca
     9781 acggccgatc tggaagatga tcgcccttct ctgccagatg aagtggcact cggcgtgacc
     9841 tatgacaata tcgacgacta tctggaaggg aaaaacgtac ctgaacaggt cgccagaaca
     9901 atagagaact ggtatctgaa aaccgaacat aaacgccgtc cgccaattac cgttttcgat
     9961 gatttctgga aaaagtaata atttgcacat aatggtttgt gcgaaaaaga gtaatttgtt
    10021 cacgccggat gcggcgtgaa cgccttattc gacctacaaa actatgcaaa ttcaatatat
    10081 tgcaggagtc gaataggcct gacaggcgta gcacgtcaga cggtgtaacc tttgtcatcg
    10141 acccgcctct tttttaatcg ctccccgcct gttacactgg atagataacc agcattcgga
    10201 gtcaacagtg gtacggcgtt taacttctcc gcggctcgaa tttgaagctg cggcaattta
    10261 tgaatatccc gaacatttac gttcattcct taatgactta cccacccgac caggggtgta
    10321 tctgtttcat ggcgaaagtg acaccatgcc gctctatatc ggcaaaagcg ttaatatccg
    10381 cagtcgggtg ctttctcatt tacgcacccc ggatgaagcc gccatgctac ggcaatcccg
    10441 gcgaattagc tggatctgta ccgcaggcga aatcggcgct ctgctccttg aagcgcgatt
    10501 aatcaaagaa caacagccgc tgtttaataa acggttgcgc cgcaatcgcc agctctgtgc
    10561 cctgcaatta aatgaaaagc gcgtcgatgt ggtgtatgcc aaagaggtgg atttttcacg
    10621 agcccccaac ctgtttggcc tgtttgccaa taggcgcgca gctttgcaag cattgcagac
    10681 catcgctgat gaacaaaaac tttgttatgg cctgctggga ctggaaccgt taagtcgcgg
    10741 tcgtgcatgt tttcgttcag cgctaaaacg ttgcgccgga gcatgctgcg gtaaagagag
    10801 ccatgaggaa catgcgctac gcttgcgcca gtctctggag cgtttgcggg tggtgtgttg
    10861 gccttggcaa ggtgcggtgg cactgaaaga acagcacccg gaaatgactc aatatcatat
    10921 tattcaaaac tggctgtggc tgggggcggt taattcgctg gaagaagcga caacgttaat
    10981 tcggacaccc gccgggtttg atcacgacgg ttataaaatt ctttgtaagc cgctgctttc
    11041 cggtaactat gaaattactg aacttgatcc ggcgaatgac cagcgagcca gttgatttca
    11101 ctgaacaaca gtttaccttg cggttgtaaa agccgcaggg tgtgcctgcc atcgaaccag
    11161 cacgcgccct ggtcggtagt gagcaattta tcgcccagtt gccacgcccc gttaatcaca
    11221 aacaccaccc cgccgcgtga gccaaaggta gtaaacgtac gttcggcaat tctgactttc
    11281 gccttacaga catccaggcg tgtcatgatg ttgaaatcca tcgacatctg cccttccgtc
    11341 agtttcgctt ttaccacctg gtccgctgca aaggcaaaag gctgtaacgg ttttaaggta
    11401 tggttaaagc ggtctgcgct ttcaaggagc atctcgccgc cttccagcaa cgtcactatc
    11461 ctttccatgc cgggaaacaa agaaaactcc ccattcgccg cgatggatgc aatgctggca
    11521 cgccagtaaa aatcacgttt tgccggtggg aaagtgcaaa tttcgcgcgt ttcaccggca
    11581 gcatttcgcc acagattcac cgacatttta cgcatatcaa agtattccat actcgctccc
    11641 ggcccgttct tttcattgtt atcgttcagg taaccgtgga tggttatcgg caactcgggt
    11701 gatttacgtt agtggtgatc aggctttttt tacaactgtt tgagaagaga aaagaaaacc
    11761 gccgatcctg tccaccgcat tactgcaagg tagtggacaa gaccggcggt cttaagtttt
    11821 ttggctgaaa gattattcag cagttgcagg cattttacct tttg
//
LOCUS       DADMKF010000122        11808 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.124, whole genome shotgun sequence.
ACCESSION   DADMKF010000122 DADMKF010000000
VERSION     DADMKF010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11808
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.124"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            <1..488
                     /locus_tag="JNC24_003056"
     CDS             <1..488
                     /locus_tag="JNC24_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAY9159745.1"
                     /translation="NVRRNLTILDMFGPPKTNAGIRTVTLLQPALEALKEQYKLTGHH
                     RKSEITFYHREYGRTEKQKLHFVFMPRVCNEKQKPYYSVSSLGARWNAAVKRAGIRRR
                     NPYHTRHTFACWLLTAGANPAFIASQMGHETAQMVYEIYGMWIDDMNDEQIAMLNARL
                     S"
     gene            540..1475
                     /gene="ttcA"
                     /locus_tag="JNC24_003057"
     CDS             540..1475
                     /gene="ttcA"
                     /locus_tag="JNC24_003057"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HAY9159746.1"
                     /translation="MQENQQITKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(1604..2977)
                     /gene="dbpA"
                     /locus_tag="JNC24_003058"
     CDS             complement(1604..2977)
                     /gene="dbpA"
                     /locus_tag="JNC24_003058"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HAY9159747.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAVISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVGQSALSLHGDLE
                     QRDRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTAR
                     AGNSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVPLEAEMATLCIDGGKK
                     AKMRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKT
                     SRVRLLK"
     gene            complement(3455..4438)
                     /gene="zntB"
                     /locus_tag="JNC24_003059"
     CDS             complement(3455..4438)
                     /gene="zntB"
                     /locus_tag="JNC24_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HAY9159748.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRLGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            4693..5925
                     /gene="dgcM"
                     /locus_tag="JNC24_003060"
     CDS             4693..5925
                     /gene="dgcM"
                     /locus_tag="JNC24_003060"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
                     /protein_id="HAY9159749.1"
                     /translation="MITHNFNTLDLLTSPVWIVSPFEEQLIYANSAARLLMQDLTFSQ
                     LRTGPYSVSSQKELPKYLSDLQNQHDIIEILTVQRKEEETALNCRLVLRKLTEAEPVI
                     IFEGIEASATLGLKASRSANYQRKKQGFYARFFLTNSAPMLLIDPSRDGQIVDANLAA
                     LNFYGYNHETMCQKHTWEINMLGRRVMPIMHEISHLPGGHKPLNFIHKLADGSTRHVQ
                     TYAGPIEIYGDKLMLCIVHDITEQKRLEEQLEHAAHHDAMTGLLNRRQFYHITEPGQM
                     QHLAIAQDYSLLLIDTDRFKHINDLYGHSKGDEVLCALARTLESCARKGDLVFRWGGE
                     EFVLLLPRTPLDTALSLAETIRVSVAKVSISGLPRFTVSIGVAHHEGNESIDELFKRV
                     DDALYRAKNDGRNRVLAA"
     gene            complement(5946..6509)
                     /gene="smrA"
                     /locus_tag="JNC24_003061"
     CDS             complement(5946..6509)
                     /gene="smrA"
                     /locus_tag="JNC24_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HAY9159750.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRREGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMM
                     FSFIQQAMADGLRNVLIIHGKGRDDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
     gene            complement(6839..7747)
                     /locus_tag="JNC24_003062"
     CDS             complement(6839..7747)
                     /locus_tag="JNC24_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY9159751.1"
                     /translation="MAFQVKIHQIRAFVEVARQGSIRGASRMLNMSQPALSKSIQELE
                     EGLAAQLFFRRSKGVTLTDAGESFYQHASLILEELRAAQEDIRQRQGQLAGQINIGMG
                     ASISRSLMPAVISRFHQQHPQVKVRIMEGQLVSMINELRQGELDFTINTYYQGPYDHE
                     FTFEKLLEKQFAIFCRPGHPAIGARSIKQLLDYSWTMPTPHGSYYKQLSELLDDQAQT
                     PQVGVVCETFSACISLVAKSDFLSILPEEMGCDPLHGQGLVMLPVSEILPKAAYYLIQ
                     RRDSRQTPLTASLITQFRRECGYLQS"
     gene            7923..9233
                     /locus_tag="JNC24_003063"
     CDS             7923..9233
                     /locus_tag="JNC24_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallo-hydrolase"
                     /protein_id="HAY9159752.1"
                     /translation="MESLNQFVNSLAPKLSHWRRDFHHYAESGWVEFRTATLVAEELQ
                     QLGYSLALGREVVNESSRMGLPDELTLQREFERARQQGALAQWIAVFEGGFTGIVATL
                     DTGRPGPVMAFRVDMDALDLSEEQDVSHRPYRDGFASCNAGMMHACGHDGHTAIGLGL
                     AHTLKQFESGLHGVIKLIFQPAEEGTRGARAMVDAGVVDDVDYFTAVHIGTGVPAGTV
                     VCGSDNFMATTKFDAHFTGTAAHAGAKPEDGHNALLAAAQATLALHAIAPHSEGASRV
                     NVGVMQAGSGRNVVPTSALLKVETRGASDVINQYVFDRAQQAIQGAATMYGVGVETRL
                     MGAVTASSPSPQWVAWLQNQAAQVAGVNQAIERVEAPAGSEDATLMMARVQQHQGQAS
                     YVVFGTQLAAGHHNEKFDFDEQVLAIAVETLARTALNFPWTRGI"
     gene            9233..10678
                     /locus_tag="JNC24_003064"
     CDS             9233..10678
                     /locus_tag="JNC24_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HAY9159753.1"
                     /translation="MQEIYRFIDDAIEADRQRYTDIADQIWDHPETRFEEFWSAEHLA
                     SALESAGFTVTRNVGNIPNAFIASFGQGKPVIALLGEYDALAGLSQQAGCAQPTSATP
                     GENGHGCGHNLRGTAAFAAAIAVKKWLEQYGQGGTVRFYGCPGEEGGSGKTFMVREGV
                     FDDVDAALTWHPEAFAGMFNTRTLANIQASWRFKGIAAHAANSPHLGRSALDAVTLMT
                     TGTNFLNEHIIEKARVHYAITNSGGISPNVVQAQAEVLYLIRAPEMTDVQHIYDRVAK
                     IAEGAALMTETTVECRFDKACSSYLPNRTLENAMYHALSHFGTPEWNSEELAFAKQIQ
                     ATLTPNDRQNSLNNIAATGGENGKAFALRHRETVLANEVAPYAATDNVLAASTDVGDV
                     SWKLPVAQCFSPCFAVGTPLHTWQLVSQGRTSIAHKGMLLAAKTMAATTVNLFIDSGL
                     LQECQQEHQQVTDTQPYHCPIPKNVTPSPLK"
     gene            10715..>11808
                     /locus_tag="JNC24_003065"
     CDS             10715..>11808
                     /locus_tag="JNC24_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate transporter"
                     /protein_id="HAY9159754.1"
                     /translation="MSMSSIPSSSQSGKLYGWVERIGNKVLHPFLLFIYLIIVLMVTT
                     AILSAFGVSAKNPTDGTPVVVKNLLSVEGLHWSLPNVIKNFSGFAPLGAILALVLGAG
                     LAERVGLLPALMVKMASHVNARYASYMVLFIAFFSHISSDATLVIMLPMGALIFLAVG
                     RHPVAGLLAAIAGVGCGFTANLLIVTTDVLLSGISTEAAAAFNPQMHVSVIDNWYFMA
                     SSVVVLTIVGGLITDKIIEPRLGQWQGNSDEKLQTLTESQRFGLRIAGVVSLLFIAAI
                     ALMVIPENGILRDPINHTVMPSPFIKGIVPLIILFFFVVSLAYGIATRTIRRQADLPQ
                     LMIEPMKEMAGFIVMVFPLAQFVAMFNWSN"
BASE COUNT         3007 a         2999 c         2925 g         2877 t
ORIGIN      
        1 tgaatgtcag aagaaacctg acgatacttg atatgttcgg tcccccaaaa acaaatgccg
       61 ggatccggac ggtaacatta ctgcagccgg ctcttgaagc actgaaggag caatacaaac
      121 tgaccgggca tcatcgcaaa agcgaaatca ctttttatca tcgggagtac ggcagaactg
      181 aaaagcaaaa actgcatttt gttttcatgc ccagggtgtg taacgaaaaa cagaaacctt
      241 attactcggt aagcagtttg ggtgcaagat ggaatgcagc agtaaaacgt gctggtattc
      301 gccgccgtaa tccgtaccat acgcgacata cttttgcctg ctggctgttg acggcaggag
      361 cgaacccggc atttatagcc agccagatgg ggcatgaaac tgcgcaaatg gtgtatgaaa
      421 tttacggtat gtggattgat gacatgaacg acgaacagat agccatgttg aatgcgcggt
      481 tatcgtagtt gcaaagtttg cccccaattt gccccattta gtaccagaga actgaaataa
      541 tgcaagaaaa tcaacaaatt acaaagaaag aacaatacaa cctgaacaaa ttacaaaaac
      601 gtctgcgtcg taacgtgggc gaagccattg ctgacttcaa tatgattgaa gaaggcgatc
      661 gcatcatggt ttgcctctcc gggggtaaag acagctatac catgctggag attctgcgca
      721 atttgcagca aagcgcgcca atcaattttt cgctggtggc tgttaacctc gatcaaaagc
      781 aaccgggctt cccggaacac gttctgcccg agtatcttga aaagctgggc gttgagtaca
      841 agattgtcga agagaacact tacggtatcg tgaaagagaa gattccagag ggcaaaacca
      901 cttgctcact gtgttctcgc cttcgtcgcg gtatccttta tcgtaccgca acggaactgg
      961 gggcgacgaa gatcgcgctg ggtcaccatc gtgacgatat cctgcagacg ctgttcttaa
     1021 atatgttcta cggcggcaag atgaaaggta tgcctccgaa actgatgagc gatgatggca
     1081 aacatatcgt tattcgtccg ctggcctact gccgcgagaa agatattcag cgatttgccg
     1141 atgcaaaagc gttcccgatt attccgtgca acctgtgcgg ttcacagcct aacctgcaac
     1201 gtcaggtgat tgctgacatg ttgcgtgact gggataaacg ttatccaggg cgtatcgaga
     1261 cgatgttcag cgcgatgcag aatgtggtgc cgtcgcatct gtgcgatacc aacctgttcg
     1321 atttcaaagg catcacccac ggttctgaag tggttaacgg gggtgatctg gcgtttgatc
     1381 gcgaagagat cccactacaa ccggcgggct ggcagccaga agaagatgaa aatcagttgg
     1441 atgagttacg gctgaatgtg gttgaagtga aataaccagg atggcgcccg atgcgcaagc
     1501 gtatcgggct actcttatgg aggccggata agacgcggcc agcgtcgcat ccggcaatcc
     1561 cgaataagat gtttactctt gcacccggca attcaacatt tcattatttt aataaccgca
     1621 cccggctcgt ttttccttta atcttcccgc cctgtaactg tttccatgct ttatgagcaa
     1681 cagcctgacg gaccgcgaca tagacatgcg ccggatgcac ggcgattttg ccaatatctg
     1741 cgccatcaag cccgatatct cctgtcagtg cacctaatac atcacccggg cgcattttgg
     1801 cttttttccc accatcgata cacaacgttg ccatttctgc ttccagcggc acaatggaac
     1861 tattagctgg cggcgtttgc cagttaagtt ttatctgcaa catgtcagaa atgatattgg
     1921 cccgctgtgc ttcttccgga gcacagaaac tgatcgccag accgctattt cctgcacgag
     1981 ctgtacggcc gatgcgatgt acatgaactt cagggtccca cgccagctca aagttcacca
     2041 ccagctcaag cgatttaata tccagaccac gcgcagcaac atcagtcgca accagtacac
     2101 gggcgctacc gttagcaaaa cgtaccaggg tctgatcgcg atcgcgttgc tccaaatcgc
     2161 cgtgtaatga caatgcactt tgccctactt cattcagcgc gtcgcagaca gcctggcaat
     2221 cttttttggt attgcaaaac accacgcaag aggatggctg atgcaagctt aataaccgtt
     2281 gcaacagagg aattttgcct ttgctggatg tctcataaaa ttgttgttca atgggtggca
     2341 aagcatctgt tgagtcaatt tcaatcgcca aaggatcgcg ttgcactcgt ccgctgatta
     2401 cggcaatggc ttccggccag gttgccgaaa acagaagcgt ctgtcgagat gcaggcgcaa
     2461 aacggatgac atcatcaatg gcatcgctaa atcccatatc cagcatgcgg tcggcctcat
     2521 ccatcaccaa agtattcagc gcatccagtg ataccgtgcc tttttgcaag tgatccagca
     2581 aacgacccgg cgttgccacg ataatatgcg gcgcatgttg caacgaatca cgctgcatac
     2641 cgaacggttg accaccgcac aacgtcaaaa ttttggtatt tggcagaaaa cgcgccagcc
     2701 gacgcaattc acctgccacc tgatccgcca gttcacgcgt aggacacagc actaaagcct
     2761 gggtttgaaa tagcgacgca tcaatttgct gtaacaagcc gaggccaaaa gccgccgttt
     2821 tgccgctgcc ggttttcgcc tgcacgcgaa catcttttcc ggcaaggatc gccggaagcg
     2881 cggcggcctg caccggcgtc atagttaaat aacccaactc attaaggttc gtgagttggg
     2941 cgggaggcaa aacattcagg gtagaaaaag cggtcacaat ctattctcgt ggtcatcgac
     3001 gcaaagttag caggcgcgta tcctcgcaga tctacgctca cgatgcgaca atttaatcgg
     3061 ttcttcatcg ggtggtgggt caggcatggg ttgcgggcga gggatcggat cgggcactgg
     3121 aacaggatcg ccaggaatcg gttcagggac aggaatttgc aaataaataa gtgtcgtcat
     3181 atttccctcc ggtcattggg tggactctta aagggtagac gctgataaat aacaggcaaa
     3241 aaaaagccga ctcatcaaag tcggcgtcgt acgaatcaat tgtgctatgc agtaattcaa
     3301 aaaaggaagt aagacaatat ggagcgcaac gcccatcgct tgacgttgca ttcacctgca
     3361 agggagatat tgccctgaat gggtagagag tttattgact tcgctcaaac tttgcggcgt
     3421 ttttgtatac agacagccgg aaaaattgct tttgttacaa ccatttacta cggtgcaacc
     3481 ataaagcaac accaccaata agaacaacta acagaataca aaaaattgaa aatccgaatt
     3541 gccacccgcc gccagggatc ccaccaaggt tgacgccaaa taacccggtc agaaaggtac
     3601 tgggtaaaaa gaccattgcc atcaacgaca ttgtataggt acgacgagct aaattttcct
     3661 gcatcacctg agcgatttca tccgccatca cgccagtccg tgctatacag gcgtcgattt
     3721 cgtcaaggcc gcgcccaagg cgatcggcaa tatcctgcat ccgacggcgt tggtcatcgc
     3781 tcatccacgg caaacgttca ctggcaagac gagcataaac atcacgttgc ggtgccatat
     3841 agcgacgcat cacaattaat tgtttgcgca gcagagccag gaatccacgc ggtggaattt
     3901 gctgatcaag gagattatct tcaaggtcga taattttatc gtgcagctgc tcgataaatt
     3961 cgctggaatg atcggtcaac gcatcgcaca catccaccag ccaccctccg caatcggtcg
     4021 gacccgtgcc ctcttccaga tcgctcacca catcgtccag cgccagcact ttgcgttgtc
     4081 gggtcgaaac aattaaccgc ccgtccatat atacacgcat ggcgaccagt tgatcggggc
     4141 gttcatcggt gctgccgttt atacagcgca atgtaatcag cgtgccttca ccgagacggc
     4201 tgactcgggg ccgcgtgctc tcgcccgcca gcgcatcacg tacgttattg ggaagcagcg
     4261 gtgttgtcgc cagccattgg gcgctatcat ggtgtacata attaaggtgg agccagcagg
     4321 gatgcgcttc atcaatcaca tctgtatttt ccagcggttt aacgccgcct ctaccatcca
     4381 gcatccaggc aaatactgca tccgggacat taacgtccga tcccttaatc gcttccacag
     4441 tgcctccatc atcaacgcat tattttgtag tctagccttc tggccctgtt acgcaacatc
     4501 tcatcacccc attaccctga aatgattaat aaaattctgt ctaaattgaa tacaaaaagc
     4561 aaaatgcttt tccgtataca aaccgtgtga agtgttaaat agcgtctatc attatcagaa
     4621 ttatctgatc atatgacgtg gcttttttgc gatcggatag caacaaaaat tgataaaaat
     4681 aacgggatct caatgattac gcacaacttc aataccctgg acttactcac cagtcctgtc
     4741 tggatcgttt cgccctttga ggaacagtta atttatgcca atagcgcggc gcgactgttg
     4801 atgcaagacc tcacgtttag tcagctacga accggtccct attccgtctc ctcacaaaaa
     4861 gaactgccga aatacctctc cgatctgcaa aaccaacacg atattatcga aatcctcact
     4921 gttcagcgta aagaagagga aacagcatta aactgtcggc ttgttttgcg aaagctgaca
     4981 gaagcagaac cggtgattat tttcgaaggt atcgaagcgt cggcaacgct gggtttaaaa
     5041 gccagtcgct cggcaaatta tcagcgcaaa aaacaaggtt tttatgcgcg cttttttctg
     5101 actaactctg caccaatgtt gttgattgac ccctcacgag atggccaaat cgtcgatgct
     5161 aacctcgccg cgctcaattt ctatggttat aaccatgaaa cgatgtgcca gaaacatacc
     5221 tgggaaataa atatgctcgg gcgtcgcgtc atgcctatca tgcatgaaat ttcgcattta
     5281 cccggtggtc ataaacctct gaattttatt cataagctgg cggatggttc gactcgtcat
     5341 gtgcagacct acgccgggcc gattgaaatt tatggcgaca agctcatgtt atgtattgta
     5401 catgatatta ctgagcaaaa acggctggag gagcagctgg aacatgctgc ccaccatgac
     5461 gcgatgaccg gattactgaa tcggcgacag ttttatcaca ttaccgagcc aggccaaatg
     5521 cagcatctcg ccatcgccca ggattacagc ttgttgctca tcgacaccga tcgttttaaa
     5581 cacattaacg atctctatgg gcattctaaa ggtgatgagg tgttatgcgc cctcgcccgc
     5641 accctcgaaa gttgcgctcg caaaggcgat ttggtgtttc gttggggagg cgaagagttt
     5701 gtcttattgc taccaagaac cccactggat accgcgcttt cgctggctga aactatccgc
     5761 gtaagcgtgg caaaagtgag tatttcgggc ttaccacgct ttaccgtcag cattggtgtg
     5821 gcgcatcacg aaggaaatga aagcatcgat gaactgttta aacgcgttga tgatgctttg
     5881 tatcgggcga aaaatgatgg acgcaaccgc gtgctggcgg cataagccgc ggatgcgtct
     5941 cgagatcaac gactgcgctt agcgtggcgc tcccagtttt cttgcttcgc ctgcgccgtt
     6001 ttacgtagtg cgacgtaaca cgccccgctg ccgccatgat gcggtagcgc agtgcaatat
     6061 gcctgaacat catcaaattc ggtcagccag cgcgccacat agctgcggac aatattggca
     6121 tgcgatttat catcccgtcc tttgccatga ataatcagca cgttacgcaa accatcagcc
     6181 atggcttgtt gaatgaaact gaacatcatt ttgcggcact cttccaccgg ctggcgcaaa
     6241 agattcaggc tcgcctgttg cggatattta ccactgcgca gcttatccag caccccatgt
     6301 tgcaaccctt cccgccgaaa ctccagcggc tgacttagtg ggatgatgtc gagaaatccg
     6361 gtggtgagga aattatcaag ctgcagcgtg tcgatacgct gcggcgcacg ttggttacgc
     6421 gttggatgcc agtggacatc ggtagcacgt ttcagcggct ggacatcttc catggcgtca
     6481 agaaacagcg atttgtcgtc aaggttcatg ttacatcctc ccgcaattaa gagcgcgata
     6541 tgataaccag accgggtcgg tccaacaacg tattacccaa aattccagta ataagttcca
     6601 aatattgccg atattttaag caaaatgctt atgcatgatt attcattcac gatattaata
     6661 atgtaactta tattttcgtg aaatctgtca ctgaagaaaa ttggcaacta aaggttaaaa
     6721 ccgttataac acagtcaccg gcgcagagga gacaatgccg gatttaagac gcggatgcac
     6781 tgctgtgtgt actgtagagt ctggcggatg tcgacagact ctattttttt atgcagtttt
     6841 aactttgcag atagccgcat tctcgccgga attgcgtgat taatgacgcg gtcagtggtg
     6901 tctggcgact atcacgccgc tgaatcaaat aataggccgc tttcggtaaa atttcgctaa
     6961 ccggcaacat caccagcccc tgtccgtgca agggatcgca gcccatttct tcaggcagta
     7021 tgctgagaaa atcgcttttt gccaccagac tgatacaggc tgagaacgtc tcgcagacta
     7081 caccgacctg tggcgtttgc gcctgatcgt caagcaattc actcaactgt ttgtagtaac
     7141 tgccgtgtgg cgtcggcatt gtccagctgt aatccagtaa ctgtttgatc gaacgggcac
     7201 caatggcggg gtgtcccggg cggcaaaaga tcgcgaattg cttttccagt aatttctcaa
     7261 aagtaaattc gtggtcgtac ggtccctgat aataggtatt gatggtgaaa tccaattctc
     7321 cctgacgcaa ttcattaatc atcgacacca gttgcccttc cataatgcgt acttttacct
     7381 gcggatgctg ctgatgaaaa cgagatatga cagctggcat cagactgcgg gaaatactgg
     7441 cccccatgcc gatattaatc tgccctgcca gttgcccttg tcgttggcga atatcctctt
     7501 gggctgcgcg cagctcttca agaataagac tggcgtgctg ataaaaactt tcaccggcat
     7561 cagttaacgt cacgccttta ctacggcgaa aaaagagttg cgccgctaat ccttcttcca
     7621 gctcctgaat agatttactc agtgccggtt gcgacatatt caacattcgg ctcgctccgc
     7681 gaatgctgcc ctgacgagcc acttcaacaa aagcccgaat ttgatgaatt tttacctgaa
     7741 aagccatgac gccaccgata accgttattt atcagaccaa agaaactggc atctacttta
     7801 atgcagatga ttgtgtcagg gtaatttatg aacggttaaa actgtgaaaa atcagttagt
     7861 gataagtaaa aactatcgct acgtgaaccg ggtcacactt tttactgatg acgggaaagg
     7921 ttatggagtc tttgaatcaa tttgttaatt cgcttgcccc aaaattatcg cactggcgac
     7981 gtgattttca tcactatgca gagtctggct gggtggaatt ccgcactgcc acccttgttg
     8041 cggaagaatt gcagcagctc ggctattcac tggcgctggg ccgcgaagta gttaatgaaa
     8101 gtagccggat gggactacct gatgaactca ctctacaacg cgaattcgag cgcgctcgtc
     8161 aacagggtgc gctagcacaa tggattgcgg tttttgaagg tggtttcact ggtatcgtcg
     8221 ccaccctgga taccggtcgc cccggtccgg tgatggcttt ccgtgtcgat atggacgcgc
     8281 tggatctcag tgaagagcag gatgtcagcc atcgccccta ccgcgacggt tttgcgtcat
     8341 gtaacgccgg aatgatgcat gcctgtggtc atgatggaca taccgccatt gggcttgggc
     8401 tggcgcatac ccttaaacaa ttcgagtccg gactacatgg cgtcatcaaa ctgatttttc
     8461 agcctgcaga ggaaggtacg cgtggcgcgc gggcgatggt cgatgcaggt gtcgtagatg
     8521 atgttgatta ttttactgcc gtgcacattg gcactggcgt acctgcgggc accgtggtgt
     8581 gcggcagtga taattttatg gcaaccacca aatttgacgc gcacttcacc ggtaccgccg
     8641 ctcacgcagg cgcaaaacca gaagacggtc acaatgcctt gttggcggca gcacaagcca
     8701 ctcttgcact gcatgcaatc gccccgcaca gcgaaggagc ttccagagta aacgtgggcg
     8761 ttatgcaggc aggaagcggt cgtaacgttg ttcctacctc ggcgttgctg aaagtggaaa
     8821 cacgcggggc cagcgacgtc attaatcaat atgtttttga ccgtgcacaa caagcgattc
     8881 agggcgcagc aaccatgtat ggtgtcggcg ttgaaactcg tctgatgggt gcagttaccg
     8941 ccagttctcc ttcgccgcaa tgggtcgcat ggttgcaaaa tcaggcggct caggtcgcgg
     9001 gggtcaatca ggccattgaa cgtgttgaag cgcctgcggg ttccgaagat gccacattaa
     9061 tgatggcccg cgtgcagcaa catcaagggc aagcctccta cgtggtgttt ggcacacagc
     9121 tggcggcagg tcatcacaac gaaaaattcg attttgacga gcaggttctc gctattgccg
     9181 tcgaaacgct ggcgcgcacc gcgctcaatt ttccctggac gcgaggtatc tgatgcagga
     9241 aatctatcgt tttatcgacg atgcgattga agccgatcgc caacgttata ccgatattgc
     9301 cgatcaaatc tgggatcatc cagaaacacg ttttgaagag ttctggtcag cggagcatct
     9361 ggcttcggct ctggaatctg caggcttcac cgttacccgc aacgtaggca atatcccaaa
     9421 tgcctttatt gcttcgtttg gtcaaggcaa accggttatc gccctgctgg gagaatatga
     9481 cgccctggca ggtttaagtc agcaagctgg ttgcgcgcaa cctacatccg cgacgcccgg
     9541 tgaaaatggt cacggttgcg gacacaattt gcggggaacc gccgcctttg ccgctgcaat
     9601 agccgtcaag aaatggctgg aacaatatgg gcaaggcggc acagtgcgct tttatggttg
     9661 tcctggcgaa gaaggcggct cgggtaaaac gttcatggtc cgcgaggggg tatttgatga
     9721 tgtggatgcg gcactcacct ggcacccgga agcctttgcc ggtatgttca atacccgcac
     9781 gctggcaaac attcaggcat catggcgctt taaagggatc gcagcacatg ccgcgaattc
     9841 ccctcatttg ggacgcagcg cccttgatgc cgtaacgttg atgaccactg gcaccaactt
     9901 cctcaacgaa catattattg aaaaagcgcg cgtacactat gccatcacaa atagcggcgg
     9961 gatctcgccc aacgtggtcc aggcgcaggc agaagtgctt tatcttatcc gcgcccccga
    10021 aatgaccgac gtgcagcata tttatgatcg ggtcgccaaa atcgccgaag gtgcggcatt
    10081 gatgaccgaa accacggttg aatgccgctt cgacaaagcc tgttccagtt atctcccgaa
    10141 tcgcacctta gaaaatgcca tgtaccatgc cctatcccat tttggtaccc cggaatggaa
    10201 ctccgaagaa ctggcttttg cgaaacaaat tcaggctacg ctcaccccca acgatcggca
    10261 aaacagtctg aataatatcg ccgcaaccgg tggcgaaaac ggcaaggctt ttgcactacg
    10321 tcatcgtgaa acggtactgg cgaatgaggt cgctccatat gccgccaccg ataacgtgct
    10381 tgcggcatcg actgatgtcg gcgacgttag ttggaaactg cctgttgccc agtgtttcag
    10441 cccctgtttt gccgtcggta caccgctaca tacgtggcaa ctggttagcc aggggcgaac
    10501 atctattgct cataaaggaa tgctgctggc ggcgaaaact atggcagcaa ccacagtcaa
    10561 tctcttcatt gattcagggc tattgcaaga atgccaacaa gagcatcagc aagtaacgga
    10621 cacgcaaccg tatcactgcc ctatcccgaa aaacgtgaca ccgtcacctt taaaataaca
    10681 acaacaacgc aaacacaaca accgaggaat gcccatgagt atgtcatcca taccgtcgtc
    10741 ctcccaatcc gggaagctct atggctgggt cgaaagaatt ggtaacaagg ttctccatcc
    10801 ttttttgctc tttatctatt tgattatcgt actcatggtg acgacggcaa ttttgtcggc
    10861 ctttggcgtc agtgcgaaaa acccgaccga tggaacgccg gtggtggtga aaaacctgct
    10921 cagtgtggaa ggattacact ggtctttacc caatgttatt aaaaacttta gcggttttgc
    10981 tccacttggt gcgatcctgg cgctggtttt aggtgccggt ctggcggagc gcgtcggctt
    11041 actgccagcg ctaatggtta aaatggcatc gcatgttaat gcccgctacg ccagttatat
    11101 ggtgctgttt attgcttttt tcagccacat ttcttccgat gcgacgttag tgatcatgct
    11161 accgatgggt gcgctgattt ttctggcggt gggcaggcat ccagttgcag gtttactggc
    11221 tgccattgca ggcgtaggtt gcggctttac ggctaattta ctgattgtca caaccgacgt
    11281 gttgctgtcg gggatcagca cggaagcggc ggctgcgttc aatccgcaaa tgcacgtcag
    11341 tgtaattgat aactggtatt ttatggccag ctccgtagtc gtactgacga ttgttggcgg
    11401 cctgataacc gacaaaatca tcgagccacg gttaggtcaa tggcagggaa acagcgatga
    11461 gaaactgcag acattgaccg aaagtcagcg ttttggttta cgcatagcag gtgtcgtatc
    11521 gctacttttt attgctgcga ttgcgctgat ggtgatcccg gaaaacggga tattgcgcga
    11581 tccgattaat cacaccgtga tgccatcacc ctttattaaa ggtatcgtgc cactgatcat
    11641 tctttttttc tttgttgtct cgctggctta tggcatcgct acccgcacaa ttcgacgtca
    11701 ggcggattta ccgcagttaa tgattgaacc gatgaaagag atggcaggat ttatcgtgat
    11761 ggtttttccc ctcgcccagt ttgtcgccat gtttaactgg agcaacat
//
LOCUS       DADMKF010000123        11779 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.125, whole genome shotgun sequence.
ACCESSION   DADMKF010000123 DADMKF010000000
VERSION     DADMKF010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11779
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.125"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(<1..611)
                     /locus_tag="JNC24_003066"
     CDS             complement(<1..611)
                     /locus_tag="JNC24_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HAY9159755.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLMEVTPVIIV
                     VPLLLATAKRYPLTPLLYTLIFFHAIILMVGGQYTYAKVPIGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVIFLARFHCRQLRR"
     gene            complement(734..2380)
                     /locus_tag="JNC24_003067"
     CDS             complement(734..2380)
                     /locus_tag="JNC24_003067"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HAY9159756.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNPGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            complement(2458..3798)
                     /locus_tag="JNC24_003068"
     CDS             complement(2458..3798)
                     /locus_tag="JNC24_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter"
                     /protein_id="HAY9159757.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            complement(4369..5088)
                     /gene="dcuR"
                     /locus_tag="JNC24_003069"
     CDS             complement(4369..5088)
                     /gene="dcuR"
                     /locus_tag="JNC24_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HAY9159758.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDTPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTDWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            complement(5085..6716)
                     /gene="dcuS"
                     /locus_tag="JNC24_003070"
     CDS             complement(5085..6716)
                     /gene="dcuS"
                     /locus_tag="JNC24_003070"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     DcuS"
                     /protein_id="HAY9159759.1"
                     /translation="MRHSLPYRMLRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLANSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILKALNGKENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            6897..7127
                     /gene="yjdI"
                     /locus_tag="JNC24_003071"
     CDS             6897..7127
                     /gene="yjdI"
                     /locus_tag="JNC24_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HAY9159760.1"
                     /translation="MDQALLDEGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKPWIMPDEVDVATVVKVIDTCPSGALKYRHK"
     gene            7139..7411
                     /locus_tag="JNC24_003072"
     CDS             7139..7411
                     /locus_tag="JNC24_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAY9159761.1"
                     /translation="MEIREGHNKFYINDEQGKQIAEIVFVPTGENLAIIEHTDVDESL
                     KGQGIGKQLVAKVVEKMRREKRKIIPLCPFAKHEFDKTREYDDIRS"
     gene            7638..7934
                     /gene="ghoS"
                     /locus_tag="JNC24_003073"
     CDS             7638..7934
                     /gene="ghoS"
                     /locus_tag="JNC24_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HAY9159762.1"
                     /translation="MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEY
                     GIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDIDFDLNIMTVDDYFRQFYK"
     gene            7962..8135
                     /gene="ghoT"
                     /locus_tag="JNC24_003074"
     CDS             7962..8135
                     /gene="ghoT"
                     /locus_tag="JNC24_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system toxin GhoT"
                     /protein_id="HAY9159763.1"
                     /translation="MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITW
                     PMSLPVALLFSLF"
     gene            complement(8254..9771)
                     /gene="lysS"
                     /locus_tag="JNC24_003075"
     CDS             complement(8254..9771)
                     /gene="lysS"
                     /locus_tag="JNC24_003075"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HAY9159764.1"
                     /translation="MSEQETRGANEAIDFNDELRNRREKLAALRQQGVAFPNDFRRDH
                     TSDQLHEEFDAKNNQELESLNIEVSVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     SLPEGVYNDQFKKWDLGDIIGARGTLFKTQTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEVRYRQRYLDLIANDKSRQTFVVRSKILAAIRQFMVARGFMEVETPMMQVIPGGA
                     SARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYHDLIELTESLFRTLAQEVLGTTKVTYGEHVFDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDAAKALAESIGITVEKSWGLGRIITEIFDEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAERFQEQVNAKAAG
                     DDEAMFYDEDYVTALEYGLPPTAGLGIGIDRMIMLFTNSHTIRDVILFPAMRPQK"
     gene            complement(10008..11465)
                     /gene="dtpC"
                     /locus_tag="JNC24_003076"
     CDS             complement(10008..11465)
                     /gene="dtpC"
                     /locus_tag="JNC24_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpC"
                     /protein_id="HAY9159765.1"
                     /translation="MKTPSQPRAIYYIVAIQIWEYFSFYGMRALLILYLTHQLGFDDN
                     HAISLFSAYASLVYVTPILGGWLADRLLGNRTAVIAGALLMTLGHVVLGIDTNSTFSL
                     YLALAIIICGYGLFKSNISCLLGELYDENDHRRDGGFSLLYAAGNIGSIAAPIACGLA
                     AQWYGWHVGFALAGGGMFIGLLIFLSGHRHFQSTRSMDKKALTSVKFALPVWSWLVVM
                     LCLAPVFFTLLLENDWSGYLLAIVCLIAAQIIARMMIKFPEHRRALWQIVLLMFVGTL
                     FWVLAQQGGSTISLFIDRFVNRQAFNIEVPTALFQSVNAIAVMLAGVVLAWLASPESR
                     GNSTLRVWLKFAFGLLLMACGFMLLAFDARHAAADGQASMGVMISGLALMGFAELFID
                     PVAIAQITRLKMSGVLTGIYMLATGAVANWLAGVVAQQTTESQISGMVIAAYQRFFSQ
                     MGEWTLACVAIIVVLAFATRFLFSTPTNMIQESND"
     gene            complement(11524..>11779)
                     /locus_tag="JNC24_003077"
     CDS             complement(11524..>11779)
                     /locus_tag="JNC24_003077"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="constitutive; catalyzes the formation of cadaverine
                     from lysine; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HAY9159766.1"
                     /translation="TEEVYLDEMVGRINANMILPYPPGVPLVMPGEMITEESRPVLEF
                     LQMLCEIGTHYPGFETDIHGAYRQADGRYTVKVLKEESKK"
BASE COUNT         3044 a         2856 c         2892 g         2987 t
ORIGIN      
        1 aagcgccgta gctggcggca atgaaagcga gcgaggaata tcaccgtcgt taatgcgcca
       61 agcagcgcgc agaacatatc ggattgcgtg tcccactggt cgccctgggt gccgagaaaa
      121 tcatccgctc cctggcccat tgccagcgcc gcccaccact cgattaattc atacatcgcg
      181 cttatcgcca gcgccacgca gcagactagg aacgccacca ttttacgtcc gcgcacgtac
      241 atcccgcgca cgagaatttc tcgtgccacc agtgcaggca ccagcccctg gaaaaaatgc
      301 cccagcttgt catacggatt acggctcaac cccagccatt cctgcacctc aaaaccgatg
      361 gggactttcg cgtaggtgta ttgcccgccg accatcagga tgatggcgtg aaagaaaatg
      421 agcgtataga gcagcggcgt taacggataa cgtttagcgg tggcaagcag tagcggcacg
      481 acaataatca ccggtgtcac ttccatcagc caggtgagtt tgtcatgggc cgaaatgccg
      541 gtataaatca ggattaacgt tagcgccagt gcgccggtgt taagaattaa cggtttgagt
      601 gtgcgggtca tggtgagttc gcaagtcagg gaaaatactg actattcacc agcaagcatg
      661 aaattacaat cgcaaaagag gtggcccagg gggatcacct ggcagcatgc tgccaggcgc
      721 tgggccgaag aggttactta gtgcagttcg cgcactgttt gttgacgatt tgctggaaga
      781 agtcgttacc tttgtcatcg accaggataa acgccgggaa atcttctact tcgattttcc
      841 agatagcttc catacccagc tccggataag cgacgcattc cagatgcttg atgctctgct
      901 gcgccagtac cgccgccgga ccgccgatgc taccgaggta gaagccgccg tgtttatgac
      961 acgcgtcggt aacctgctgg ctgcggttgc ctttcgccag catgatcatg ctgccgccgt
     1021 gggattgcag cagatccaca taggagtcca tacgacctgc ggtggttggg ccaagtgaac
     1081 ctgatggata accggcagga gttttcgccg gacccgcgta gtagatcggg tgatctttga
     1141 tgtactgcgg aagttcttta ccggcgtcaa tcagctcttt cagcttggcg tgtgcaatat
     1201 ctcgacccac aataatggtg ccagtgagcg acaaacgagt ggaaaccgga tattgcgaaa
     1261 gctgggcgag gatctctttc atcgggcggt taaggtcgac cttcaccgct tcgccttcac
     1321 cggcctgacg cagttcttgt ggaatgtact gacctggatt gtgttccagt ttttcgatcc
     1381 agataccttc gcggttgatt ttcgctttaa tgttacggtc agcggagcag gagacgccca
     1441 tgcctaccgg gcaggatgcg ccgtgacgtg gcagacggat aacgcgaatg tcgtgcgcga
     1501 agtatttacc gccaaactgc gcgccaagac cgagtttctg ggcctcttcc agcagttcct
     1561 gttccagctg gacatcgcgg aacgcctgac catgttcgtt cccttccgtc ggcagttcat
     1621 cgtaatagtg agcgcttgct aatttgacgg ttttcaggtt ggtttccgca gacgtaccgc
     1681 caatcacaaa cgcgatatgg tacggcgggc aggctgcagt accgagggta cgcattttct
     1741 cgacgaggaa gtttttcagt ttgccgggag tcagcagggc tttggtttcc tggtagagat
     1801 acgttttgtt ggcagagccg ccgcctttcg caacgcaaag gaatttgtac tcatcgccat
     1861 ctaccgcgta caggtcgatt tgcgcaggca ggttagtgcc ggtgttgacc tctttgtaca
     1921 tgtccagcgc cgcgttttgc gagtagcgca ggttatcttc gatataggtg ttatagacgc
     1981 ctttcgacag cgcttcttca tcaccgccac cggtccacac gcgctggcct tttttaccga
     2041 cgatgatcgc ggtgccggta tcctggcagg tcggcagcac gcctttggcg gcgatttcgg
     2101 agtttcttaa gaattgcagt gccacgtact tgtcgttttc gctggcttct ggatcgtgaa
     2161 gaatagccgc aacctgtttc tggtgtgccg gacggagcat gaaagaagcg tcgtgaaagg
     2221 cttgctgcgc cagcagggtc agggcttctg gttccacttt caggatggtt tcgccgtcga
     2281 agtcggcaac gctaacgtaa tcggaagtga gtagatagta ttcggtattg tctttcccca
     2341 tcgggaaagg tgcctggtag ataaagggtt tgtttgacat agcttccagc ctgtaactct
     2401 gtatttgtta ttcgaaaatg gcgtgccgcg tagtcacggg cggcacgcaa agtgcattta
     2461 taagaacccg tacatcgcgg cgaagatcca gccgaagacg cacgatacgc tcacaccaat
     2521 caaccccggc agaataaagc tgtggttgat gacgaagcga ccgatgtggg tggtgccgga
     2581 acggtcaaac tgaatcgctg ccagatcgct cggataagtc ggcaggatgt aataaccgta
     2641 gcaagccggt gctgaagcca cgatgtatgc cggatcaacg ccgatcgcca gcgcgaccgg
     2701 aacaatcgcc gccagcgccg cagcctgaga gtttacaaac ttggaaacca gcagcagaac
     2761 aatggcatag gcccacggat actctttcac catttcaccc agtacgccct gaatttcaga
     2821 catatgcgca ccgaacatgg tttctgccat ccatgcgata ccgtacaccg ccacgatggc
     2881 gatcataccg gaacggaaga cttcgttttt tgagatagac gcgggattgg ttttggtcag
     2941 gataataatc agcgccccgg tcagcagcat aaacatctga ataaccagta ccatcgacag
     3001 cggtttgccg ccgaaggatg gacgcaggtc cgaatcagca ccaagaaggg cgactacagc
     3061 gattgccccg aggaaaatcc acattgccag ccagttgctt ttcggcagtt ttttgtccag
     3121 cagcgtcgcg gtatcaccgt aaacatactc acggttttcc ggtacggaga tgaatttctg
     3181 gaactcttcg tctttatcca gatctttacc acggaaccag ctgaagatac cgatcgccag
     3241 gataccgatt aacgtcgatg gaatggtgat tgccagcagg tcgaggaact caagatggcg
     3301 accatcaaag gtgacattac ccagcattgc aaccagagac acgaccgcaa ccgacaccgg
     3361 actggcgatg atccccatct gtgcaccgat agaacttgcc gccatcggac gttccggacg
     3421 gatgttgttc ttaatggcga cgtcgtagat gatcggcaga atggtgtaaa ccacatgacc
     3481 cgtaccgcaa agaatggtca gcgtacaggt cacaaacggc gcgacaattg agacatattt
     3541 cgggttgcgg cgcagcagct tctcggcaat ttgcagcatg acatcaagac cgcccgaagc
     3601 ttgcaaggtc gccgatgccg ccaccaccgc aatgataacc agcatgacat caaccggtgg
     3661 tttacctggc tgaaggtgga agacgaagac cagaatgacc agaccgatac cgcctaataa
     3721 acccagcgcg ataccaccct ttctggcacc ataaaacaga catatcagta ttatgataag
     3781 ttggatagta aataacatgt gtgaaccctc gcgataatcc tatttaaatt tttgctgaat
     3841 agatcacagt cacgttctgt tttgtatgaa ctgttttcag agcctgacta aatatccgtt
     3901 tggtcgttac cggctttagc aaatacctca cagtgaatat tggctgatta aagcagcggc
     3961 aaatttatgc actgtgataa gcggcttttt cagagagaaa gactctctgg cgatgggttt
     4021 atagtttgta ctgcgcagat gttaatgttt tgtttgctgt tatcttattg atatttatcg
     4081 gtctattttt tgttctcagg gcagatgtca ttaggtttat agattaatct gatctaccca
     4141 tttgtgggta aaaatacaca taatgcgggt gacataatag ttaattaact tttgttagcg
     4201 ttttgaaatt aaaaacaccg ttcacctgaa gagatattaa tttttagcga tgatgcaggg
     4261 ataattatat ttgatctggc acaagtttta ctgatgaagg atgtaacttg tgccaggggt
     4321 attttgcatt acggtaataa ttattactta cagataacca gcgatccgtt attggcaata
     4381 ttgtttcagt agtgagtagt gttctgcctg aatacggtaa cggtaaactg gacgccccgt
     4441 gacgccataa tggatactgg tgaacaagat gtggcagttg accagccaga tgaggtattt
     4501 acggcaggaa acacgcgaaa tgttaacctc gttggctagc tcgtcggttg aaaattcata
     4561 gtcctgatgc gcgtcaatcc actggcacag tgtgcgtaac gtctgcggcg ttaagccttt
     4621 tggcaagcga cgaggatcct gttcgttgga gctgctgccg tggattagct gatcaagctc
     4681 ggcctggtca taatactgat gtttttccag cgccattttc ttttgccgcc agtcggtgag
     4741 cgcctcttca aagcgggaag cctggaaggg tttgatcagg taatccacga caccgtaatg
     4801 cagcgaatct ttaatggttg ccgcatcggc tgcggaggag atgacaatca catcactttt
     4861 gcaacgcgcg ttatgcagga caggcagtaa atcgagcccg ttctcttttt gcatatagat
     4921 atcgagcaat atcaggtcga taggcgtatc gctattgaag ataatctctt tggctttctc
     4981 cagcgtcgag gctgttccac agcattgaaa gcctgggatt tgtgctacgt atcggcgatt
     5041 cagctccgcg accattgcgt cgtcatcgat aattaataca ttgatcatct gttcgacctc
     5101 tccccgtccc agggtatctg gacaaaaaat tgtgtgaaaa tcccgggttc cgattccacg
     5161 gcgatgctgc cgccgagatt ttctacctgt tgtttgacaa gtgctaaacc gacgcctcgc
     5221 tcgcttcctt ttgtcgagac acctttgtca aaaatgtgat cgattttgtc gggtgcgatc
     5281 cccggtccat catcgttaac ttcgcagtgc agccagccgt gacggtagtg caatgttacg
     5341 ctaatttcgc caccgggttc cggccctaat gcttccagcg cgttttctat cagatttccc
     5401 aatgtggtaa tcagcgtcgc gacctggtcc tcactgccgc tgtctggcag ctggctttca
     5461 ctgtttaaaa tcagcgtatg gcctaaatcg gtcgcgcgat taatcttgct gattaaaaaa
     5521 ccagcgataa ccggagattt gatcttaccc agcagagagc caatctcttc ctgatagtta
     5581 ttggctgttt ttagaatgta atcttccaac tgcttataac tcttcagatg caataatccg
     5641 agaatcacgt gcaatttatt cataaattcg tgggatcgtt cacgaagtgc gtcggcatag
     5701 ttgaccagac cgtcgagtcg ctgcatcagt ttacgtactt cggttttgtc cctgaaggtt
     5761 gaaatggcac cgatgataac gccattactg cgcaccggaa cggtgttgat cagtaatagc
     5821 cggtctttaa tcgtaatctc ttcgtcgcgg cgcggcgtac cgtcgcgtaa cacttccgag
     5881 acatctacca cctgtgacca tgagtggctg agcgtcgaca gtttctcatc gtcctgcgac
     5941 ttacggtaat tcagcaattc ttgtgcggca tcgttgatca gcgtgacctc gccgcgatcg
     6001 tccacggcaa cgacgccttc tttgatagac tgcaacatgg cctggcgttg ctcaaacagc
     6061 gtggagattt cgtagggttc caggccgaaa aggatttttt tcagtacctt aaccagaatg
     6121 caggtgccaa tcagtccgac cagcatgcca aataataccg accagataat gctccagcga
     6181 ctgtcattga tttgttgggt cacgcggctt aactcaaggc cgatcgccac cacgccaatt
     6241 tgtttatgat tttcatcgta gatgggggta aatacgcgta aagcctgcgc cagaaaaccg
     6301 cgattgatag cgacattttc tttgccattc agcgctttaa ggatgtcatc acctttaaat
     6361 ggctgaccaa tacgctgggc ttcaggatgc gagtagcgaa gactttgcat atcggtaacg
     6421 acaataaaca gcagatcgtt gcgtttgcgc acggcttccg cgatggcctg gatgccgctc
     6481 tcctgcggtt ttttctgcaa gccctgacgg atttccggcg agttggcgag ggtacgcgcc
     6541 actgccagtg ccttgttggc tagcccgtct cgcgtcatat cactgatttg cgagaagtaa
     6601 atcagatgca ccaccaatag caccgagaac agtaccgcac tgaccattaa gatcactgtg
     6661 gtactcaatt tcatcggacg tttgcgtaac atgcggtagg gcaatgaatg tctcatcagc
     6721 ttccttgtgt gacaaatttc ttaaacatta tctctgatga ggtgggtaat tcaaagggag
     6781 taagaatgat tggctatata ggggaagaga ttctggcaac ggaaactgcc agtgctgtat
     6841 gaagattcca gggctatgct tatagcgata atcatactga tgagagaggg aaagtcatgg
     6901 atcaggcgct actggacgag ggttatcgct gttataccgg cgaaaagatc gatgtctatt
     6961 tcaacactgc gatatgtcag cattctggca attgcgtacg tggcaacggc aagttattta
     7021 atctcaaacg aaagccgtgg atcatgccgg atgaagtcga cgtcgccact gtggttaaag
     7081 tgattgatac gtgcccgagc ggcgcgctga aataccgtca taaataagcg agggtaaaat
     7141 ggaaatacgc gaaggccaca ataaatttta cattaatgac gaacaaggca agcaaatcgc
     7201 tgaaattgtc tttgtgccga ctggagagaa tttagcgatt atcgaacata ccgatgtcga
     7261 tgaaagcctg aaagggcagg ggattggtaa acagctggtt gcaaaagtcg tggaaaaaat
     7321 gcgtcgggaa aaacgaaaaa ttatcccact atgcccattt gcgaaacacg aatttgataa
     7381 aacgcgggag tatgatgata ttcgcagttg atgggagagt acagagtcac gatatttttc
     7441 atgctctccg cggagtgatg caggagagca tctgaagggt agggggatgc acaaagaatg
     7501 ggcagagagc gcgttttttt gtcccaagtc atccccttta ctgagcaaaa aaaagaatat
     7561 ctcctatatg agaatcatca atcggggtta tcaagttttg cgtccccaga gcattcaata
     7621 ttgataggag tcatattatg gaaggtaaaa acaagttcaa tacttatgtt gtttcttttg
     7681 attatccatc atcttattcc tcagtgttct taaggttaag atcattgatg tatgatatga
     7741 atttctcttc tatcgtggct gatgaatatg ggataccacg acaattgaat gaaaattcct
     7801 tcgcaataac gacatcgtta gccgcaagtg aaatcgaaga tttaatcagg cttaaatgct
     7861 tagacttacc ggatattgat tttgacctca acattatgac agttgatgac tatttccgtc
     7921 agttttacaa gtagccagga aggataagaa gaacataaaa aatggcacta ttctctaaaa
     7981 tattaatttt ttatgtgatt ggtgtgaaca tatcctttgt cattatctgg tttatctcac
     8041 atgagaaaac acatattcgt ttacttagtg cattcctggt cggaataacc tggccaatga
     8101 gtctgcctgt ggcattactt ttttctctct tttaggagct gtagttgagt ggacccgctg
     8161 gttatcgtga aacgaagcgt ctctcctcaa aactggattt gcggaacgac gaacgaagca
     8221 gtcgcctgac tgcttcgttc attaaagtga aatttatttc tgtgggcgca tcgccgggaa
     8281 gagaataacg tcgcgaatag tatgactgtt tgtaaacagc ataatcattc ggtcgatacc
     8341 aatacccaga ccagcggttg gcggcagacc atattccagc gcagttacgt aatcttcgtc
     8401 atagaacatg gcttcgtcgt cacctgcagc tttagcgtta acctgttcct ggaaacgttc
     8461 agcctgatct tctgcgtcgt ttaattcgct aaaaccatta ccgatttcac gaccaccgat
     8521 gaagaattca aaacggtcgg tgatttccgg gttaacatca ttacggcgcg ccagcggtga
     8581 cacttctgcc ggatattccg taataaaggt tggctgaatc agatgtgctt ctgccacttc
     8641 atcaaagatc tccgtgataa tacgtcccag cccccagctt ttctctaccg taattccgat
     8701 agattcagct aatgctttag cagcatcaaa attatccagg tcggccatat cggtttctgg
     8761 acgatatttt ttgattgctt cgcgcatggt gagtttttca aacggtttgc cgaaatcaaa
     8821 cacatgctcg ccataagtga ctttagtggt acccagaacc tcttgtgcca gggtgcggaa
     8881 cagtgactct gtcagttcaa tcaaatcgtg gtaatccgca tacgccatgt agagttccat
     8941 cattgtgaac tcaggattat ggcgaacaga aataccttca ttacggaagt tacggttgat
     9001 ttcgaatacc cgttcaaaac cgcctacaac cagacgtttc agatacagct ccggcgcgat
     9061 acgcagatac atatctaaat ccagagcatt atgatgggta ataaacgggc gagcagatgc
     9121 cccacctgga attacctgca tcatcggggt ttctacttcc ataaagccgc gcgcgaccat
     9181 gaactgacgg atagcggcca gaatttttga acggacaaca aacgtttgac gggatttatc
     9241 gttagcgatg aggtccagat aacgttgacg ataacggact tcctgatcct gcagaccatg
     9301 gaatttatct ggtaaaggac gtagtgcttt agtcagcagg cgcaactcag tacagtgaat
     9361 ggaaagctca cccgtttgcg tcttaaacag cgtaccgcgg gcaccgataa tgtcacccag
     9421 atcccatttt ttaaactgat cgttataaac accttctggc aggctatctc ttgcaacgta
     9481 cagttgaata cggccaccga catcctgcaa cgttacaaag gaggctttcc ccatgatacg
     9541 acgggtcatc attcggccag caaccgagac ttcaatgttt aaggattcca gttcctggtt
     9601 attcttcgca tcaaactctt cgtgcaactg gtcagaggta tggtcgcggc gaaaatcatt
     9661 gggaaacgcc acaccttgct gacgtagtgc cgccagtttt tcgcggcgat ttctcagttc
     9721 atcgttaaaa tcaatagcct cattggctcc ccgtgtttct tgttcagaca ttttggttcc
     9781 tctaaatcca gctttcaatt tagcgtagat aaagagacag atcggtacac tgtattatct
     9841 gcctcgacta ttaataactc agtttatctt atcgaaacta acgcttacct caagttgata
     9901 tcactaataa ataaccatca aaatggttta aatgacttat taatctcgat ttactagtgt
     9961 gattaactga aagttaattt attcataacc aattgttttt acgacagtta atcgttgctc
    10021 tcctgtatca tattcgtcgg cgtgctaaac agaaaacggg tagcaaaggc caatacgacg
    10081 atgatcgcga cacaagccaa cgtccactct cccatctgag aaaagaatcg ctggtaagct
    10141 gcaattacca taccgctaat ttgcgactct gtcgtctgct gtgccacgac gcctgccagc
    10201 cagttggcga ccgcgcctgt tgccagcata taaataccgg ttaatacgcc agacattttc
    10261 agacgcgtga tttgcgcaat cgccaccggg tcaataaaga gttctgcaaa gcccattagc
    10321 gccaacccgg atatcatcac gcccattgac gcttgaccgt cagccgctgc atgtcgggca
    10381 tcaaatgcca acaacataaa gccacaagcc atcagcagta agccaaaggc aaacttcagc
    10441 cagacgcgca atgttgagtt gccgcggctt tctggcgacg ccagccaggc cagtacaacc
    10501 ccagcgagca tcactgcaat tgcattcacc gactggaata gtgctgtagg tacttcaata
    10561 ttgaatgcct gacgattcac aaagcgatcg ataaacaagc tgatggtact gccgccctgt
    10621 tgtgccagta cccagaacaa cgtcccgaca aacatcaaca atacaatttg ccaaagagca
    10681 cggcggtgtt cggggaattt gatcatcatg cgggcaatga tttgtgcggc aatgaggcaa
    10741 acgatcgcca gcaaatatcc ggaccagtcg ttctccagca gcagggtaaa aaatactggg
    10801 gctaaacaga gcatcaccac taaccagctc catactggta aggcaaattt gacgctagtg
    10861 agcgcttttt tatccatact acgcgtggac tggaaatgac gatgaccgct taagaaaatc
    10921 aacaaaccga taaacatgcc gccacccgca agggcaaagc caacatgcca tccataccac
    10981 tgagcagcca ggccgcaggc gattggggcg gcgatagaac cgatattgcc cgcagcatac
    11041 agcagcgaaa aaccgccatc acgtcgatga tcgttctcgt cgtagagctc gccaagcaaa
    11101 cagctgatgt ttgatttgaa taaaccgtag ccacaaataa tgattgccag cgccagatac
    11161 aggctaaagg ttgaatttgt atcaatgccc agcaccacat ggccaagggt cattaacagc
    11221 gcgccggcaa tcactgcagt gcggttgccg agcaggcggt cggcaagcca gccgccgaga
    11281 ataggggtaa cgtaaaccag agaagcatat gcgctgaaca ggctgatggc atggttatca
    11341 tcaaaaccaa gctgatgggt gagatagaga atgagtaagg cacgcatgcc gtaaaaactg
    11401 aagtactccc agatttggat cgccacgata tagtatatcg cgcgcggctg tgagggtgtt
    11461 ttcatgtgtt ctccttatga gcaaaaaagg gaagtggcaa gccacttccc ttgtacgagc
    11521 taattatttt ttgctttctt ctttcaatac cttaacggta tagcggccat cagcctgacg
    11581 gtatgcaccg tgaatatcgg tttcaaatcc cggatagtga gtgccgattt cacacagcat
    11641 ctgcaggaac tccagaaccg gacggctttc ttcggtgatc atttcacccg gcattaccag
    11701 aggaactccc ggcggatacg gaaggatcat attggcgtta atacgaccta ccatttcgtc
    11761 gaggtaaact tcttcggtc
//
LOCUS       DADMKF010000124        11753 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.126, whole genome shotgun sequence.
ACCESSION   DADMKF010000124 DADMKF010000000
VERSION     DADMKF010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11753
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.126"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            26..1021
                     /locus_tag="JNC24_003078"
     CDS             26..1021
                     /locus_tag="JNC24_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide 1,2-glucosyltransferase"
                     /protein_id="HAY9159767.1"
                     /translation="MNEFIKERFSYLADNKKENAPELNVSYGIDKNFLYGAGVSISSV
                     LINNSDINFVFHVFTDYVDDDYLKSFNETAKQFNTSIIVYLIDPKYFADLPTSQFWSY
                     ATYFRVLSFEYLSESISTLLYLDADVVCKGSLKPLTEIIFKDEFAAVIPDNVSTQAAC
                     AKRLNIPEMNGRYFNAGVIYVNIKKWHEANLTPYLLTLLRGETKYGSLKYLDQDALNI
                     AFNMNNIYLAKDFDTIYTLKNELHDRSHRKYQQTITDKTVLIHYTGITKPWHSWAGYP
                     SASYFNIAREQSPWKKYPLKEARTVAEMQKQYKHLFAHGEYIKGITSLIKYKLKK"
     gene            1031..1723
                     /gene="rfaY"
                     /locus_tag="JNC24_003079"
     CDS             1031..1723
                     /gene="rfaY"
                     /locus_tag="JNC24_003079"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HAY9159768.1"
                     /translation="MITSIRYRGFSFYYKDNDNKYKEIFDEILAYNFKTVKVLRNIND
                     TKVSLIDTKYCRYVFKVFAPKTKRNERFLKSFVKGDYYQNLIVETDRVRSAGLTFPND
                     FYFLAERKIFNYASVFIMLIEYVEGVELNDMPIIPEDVKAEIKASMEKLHALNMLSGD
                     PHRGNFIVSKDGVRIIDLSGKSCTAERKARDRLAMERHLGIANEIKDYGYYSVIYRTK
                     LRKFIKKLKGKA"
     gene            1749..2774
                     /locus_tag="JNC24_003080"
     CDS             1749..2774
                     /locus_tag="JNC24_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactose--(galactosyl) LPS
                     alpha1,2-galactosyltransferase"
                     /protein_id="HAY9159769.1"
                     /translation="MDLLAESITEVAVSGEIANTDRVLNIAYGIDRNFLFGAAVSMQS
                     VVMHNPDLAVKFHLFTDYIDEDYLQRVNAFTSKNANVEVRIYKVSSAFIDIFPSLKQW
                     SYATFFRLVAFQYLSETIENLLYIDADVICKGSLAGLLDINFDGDKFAAVIKDVPFMQ
                     EKPAKRLAIEGLPGNYFNAGVVYLQLEAWAKNDFMNKAIAMLASDPQHTKYKCLDQDI
                     LNILFFGHCIFISGDYDCFYGIDYELKNKSDEDYKKTITDDTKLIHYVGVTKPWNDWT
                     NYPCQKYFNEAYQASCWNDVAFIPATNEKQYQVKYQHAKKNGDTFNAFIYFIKFKLNK
                     YKRKLFG"
     gene            2859..3842
                     /locus_tag="JNC24_003081"
     CDS             2859..3842
                     /locus_tag="JNC24_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAY9159770.1"
                     /translation="MSNDYPLVSIIIPTYNSSDYITETLTKLEKQTYPNFEIVIVNDG
                     SKDNTSNVLREYGLTHSRLIIINKENGGVSSARNTGIRKAQGQFICFMDDDDEIDPNY
                     LLKMYTRQHETGGDAIYCGLYGHYIKDGVTYSPINTEFNEGSLLFDFFYKKVRFHIGC
                     LFIRKQLLEENNLFFDEDLRLGEDLDFIYRLLITCDMYAVPYYMYKHNYRENSLMNSC
                     RTITHYRHESFAHERIYSSVMQLYKGNRKEEIHTLLSQNRAYHKTRYLWNVLLNGDFE
                     LLNQLVESNEKELNDCNLSGKRDKRRAKILASKNYILWRMVRLVNRKKNKR"
     gene            3888..5141
                     /locus_tag="JNC24_003082"
     CDS             3888..5141
                     /locus_tag="JNC24_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012602581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HAY9159771.1"
                     /translation="MSFCWNEINSGIKSLILILCIFSLMTLSLWDDVATKFLHAAGII
                     SALYFLITPKKTITNNPTLLIFISLCLLGIINIIWYSHYKVSGSVYTNAYRGPMETGK
                     IALCSAFIFLVLFAKNELRTKIKFGKLILFASLATQLLFFAHAMWQHFYLNVDRVALS
                     ASHATTAGYIILFPSLLASILILKSDFRHKTTLYTINFMLSLCAVIVTETRAAILVFP
                     FFALLLIVMDSYINKRINYKLYCFIAIALLAGVFSFKDTLLMRMNNLNNDLVNYSHDN
                     TRTSVGARLAMYEVGLKTYSPIGQSLEKRAEKIHELEEKEPRLSGALPYIDSHLHNDL
                     IDTLSTRGIPGVVLTILAFSAILIYALRTAKEPYILILLFSLLVVGLSDVILFSKPVP
                     TAVFVTIILLCAYFKAQSDQCLLDK"
     gene            complement(5211..6182)
                     /gene="rfaC"
                     /locus_tag="JNC24_003083"
     CDS             complement(5211..6182)
                     /gene="rfaC"
                     /locus_tag="JNC24_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HAY9159772.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGMDWQTAREPLASLFYNRKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQMVCRAPGNELSQLTANAVKRFIEENVAMI"
     gene            complement(6186..7232)
                     /gene="rfaF"
                     /locus_tag="JNC24_003084"
     CDS             complement(6186..7232)
                     /gene="rfaF"
                     /locus_tag="JNC24_003084"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HAY9159773.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDLRVLDKEAWPLMVERYVALAYDKGVMRTAQDLPKPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            complement(7242..8174)
                     /gene="rfaD"
                     /locus_tag="JNC24_003085"
     CDS             complement(7242..8174)
                     /gene="rfaD"
                     /locus_tag="JNC24_003085"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HAY9159774.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            8388..9584
                     /gene="kbl"
                     /locus_tag="JNC24_003086"
     CDS             8388..9584
                     /gene="kbl"
                     /locus_tag="JNC24_003086"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HAY9159775.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANYPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            9594..10619
                     /gene="tdh"
                     /locus_tag="JNC24_003087"
     CDS             9594..10619
                     /gene="tdh"
                     /locus_tag="JNC24_003087"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HAY9159776.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMAEGFDIGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            10858..>11753
                     /gene="waaH"
                     /locus_tag="JNC24_003088"
     CDS             10858..>11753
                     /gene="waaH"
                     /locus_tag="JNC24_003088"
                     /EC_number="2.4.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
                     /protein_id="HAY9159777.1"
                     /translation="MMNSTNKLSVIIPLYNAGDDFRTCMESLITQTWTALEIIIINDG
                     STDNSVEIAKHYAENYPHVRLLHQANAGASVARNRGIEVATGKYVAFVDADDEVYPTM
                     YETLMTMALEDDLDVAQCNADWCFRETGETWKSIPSDRLRSTGVLTGPDWLRMGLSSR
                     RWTHVVWMGVYRRDVIVKNNIKFIAGLHHQDIVWTTEFMFNALRARYTEQSLYKYYLH
                     NTSVSRLHRQGNKNLNYQRHYIKITRLLEKLNRNYADKITIYPEFHQQITYEALRVCH
                     AVRKEPDIITRQRMIAEIFTSG"
BASE COUNT         3266 a         2471 c         2649 g         3367 t
ORIGIN      
        1 ataaaatgat attgagaaaa gttaaatgaa tgaatttata aaagaacggt tttcgtattt
       61 agcagataat aaaaaagaaa acgccccaga actaaatgtt tcctacggta tcgataagaa
      121 ttttttgtat ggtgctggcg tttcaatttc ttccgttttg attaataatt cagatattaa
      181 ttttgtcttt catgttttca ctgattatgt ggatgatgat tatttaaagt catttaatga
      241 aacagcaaaa caatttaata cctcaattat tgtatattta attgacccaa aatactttgc
      301 tgatctgccg acgtcacagt tttggtcgta cgcgacatac ttcagggtat tgtcctttga
      361 atatctgagt gaaagtattt ccacactgct gtatctggat gccgatgtcg tttgtaaagg
      421 aagtctgaag cctctcacag aaattatatt taaagatgag tttgctgcgg tcattcctga
      481 caatgttagt actcaggcgg catgtgcaaa acgcctcaac attcccgaga tgaatggacg
      541 ttatttcaat gcaggcgtta tctatgtcaa tattaaaaaa tggcatgaag caaatttgac
      601 accgtattta ctcacgcttt tacgagggga aactaaatat ggctctctta aatatttaga
      661 tcaggatgcg ttgaatatcg catttaatat gaataatatc tacctcgcga aggattttga
      721 tactatttat accctgaaaa acgaacttca tgatcgtagt catcgaaagt atcagcaaac
      781 cattaccgat aaaacagtat tgattcacta tacagggata actaaaccat ggcatagctg
      841 ggctggttat ccgtctgcat catactttaa tatcgcgcgt gaacaatctc cctggaagaa
      901 atatcctctt aaagaggcgc ggactgttgc agaaatgcag aaacaatata agcatctgtt
      961 tgcccatggt gagtatatta aaggcataac ttcattaatt aagtacaagc ttaagaaata
     1021 aattactctt atgattacaa gtatacgcta tcgcggcttc tcattttatt acaaagataa
     1081 tgataataaa tataaagaaa tctttgatga aatactggcc tataatttta aaacagtaaa
     1141 agtattacgt aatattaatg ataccaaagt atcattgatt gatacgaaat attgtcgcta
     1201 tgtttttaaa gtctttgccc cgaaaacgaa aaggaatgaa cgttttttaa aatcttttgt
     1261 aaaaggtgac tactatcaga atttgatcgt tgaaaccgat cgtgtcagaa gtgcaggtct
     1321 tacttttcct aatgattttt attttctggc tgagcgtaaa attttcaact atgccagtgt
     1381 ttttatcatg cttatcgagt atgtcgaggg cgtagagctc aatgatatgc cgatcattcc
     1441 agaagatgtt aaagcagaaa ttaaggcttc aatggaaaaa ttgcatgcct taaatatgct
     1501 atcgggcgac ccacaccggg gtaactttat cgttagtaaa gatggtgtca ggataattga
     1561 cctatccggt aaaagctgta cggcagagcg taaagctcgt gatcgtctgg ccatggaacg
     1621 tcacctgggt attgccaacg aaataaaaga ttatggctac tattcagtaa tctacagaac
     1681 caaactacgc aaatttatta aaaaattaaa aggcaaagcg taaaccacac agtcaaaacg
     1741 gaaccaacat ggatttatta gctgagagta ttactgaagt cgctgtctct ggggaaattg
     1801 ctaacaccga tcgtgtgtta aatatcgctt acggtattga ccgtaacttt ttatttggtg
     1861 cggcagtatc tatgcaatca gttgttatgc ataatccgga ccttgcggtt aagtttcatc
     1921 tctttaccga ctacattgat gaagattatc tacaacgtgt taatgctttt accagcaaaa
     1981 atgctaacgt tgaagtaaga atttataaag tctccagtgc ctttattgat atcttcccca
     2041 gcctgaaaca gtggtcttat gcaacattct tccgtttagt tgcgttccag tatctgagtg
     2101 aaactattga aaatctgtta tatatcgatg ctgatgtcat ctgtaaaggc tcattagctg
     2161 gattgcttga tattaatttt gatggggata agtttgcagc tgttattaaa gatgtgcctt
     2221 ttatgcagga aaaaccagcg aagcgtctgg ctatagaggg acttccaggg aattatttca
     2281 acgccggtgt agtatatctg cagcttgaag catgggcgaa aaatgatttt atgaataaag
     2341 ccattgctat gctggcaagt gacccgcagc acacgaagta taaatgcctt gatcaggata
     2401 ttttaaatat tctgttcttt ggtcattgta tttttattag cggcgattat gattgctttt
     2461 atggcatcga ttatgagtta aaaaataaaa gcgatgaaga ttataaaaag accattaccg
     2521 atgatactaa gctgattcat tatgttggcg taacgaagcc ctggaacgac tggacgaatt
     2581 atccctgcca gaagtatttt aatgaggctt atcaggcttc ttgctggaat gatgtggcgt
     2641 ttattccagc cacgaatgaa aagcagtatc aagtgaaata tcaacatgca aagaaaaatg
     2701 gtgatacgtt taacgctttt atttacttta ttaaatttaa attaaataag tataaaagaa
     2761 aactatttgg ataataataa agatcatgga tgtctgagtt acaataataa ttaaggatga
     2821 gtactatcat attttattag ttcaggcaag agaacacgat gagcaatgat taccctttag
     2881 tatccataat aataccgacg tataattcat ctgattacat tactgaaact ctaacgaaat
     2941 tagaaaaaca aacttaccca aattttgaaa ttgttattgt taatgatggt tctaaagata
     3001 acacatcaaa cgttttgaga gagtatgggt taacccactc tcgattaatt attatcaata
     3061 aagaaaatgg cggtgtttcg tctgccagga atacaggtat ccgcaaggcg caaggacagt
     3121 ttatatgttt tatggatgat gatgatgaga tagatcctaa ctatctgctg aagatgtata
     3181 ccagacaaca tgagacggga ggggatgcga tttattgtgg gctttatggc cattatataa
     3241 aagatggtgt tacttactca cctataaata cagagtttaa tgaaggatct ttacttttcg
     3301 acttttttta taaaaaggtt agattccata tagggtgctt gtttataaga aaacaacttc
     3361 tggaagagaa taatcttttt tttgatgaag atttacgact aggagaagat ctggatttta
     3421 tctatcgact gctaattaca tgcgatatgt atgcggttcc atattatatg tataagcata
     3481 actatagaga aaattcctta atgaactcat gtagaaccat cactcattat cgacatgagt
     3541 catttgcgca cgaaagaatc tactcttctg tgatgcagtt atacaaaggt aacaggaaag
     3601 aagaaattca tacattattg agtcaaaata gagcttatca taaaactcgt tatttatgga
     3661 atgttctact taatggtgat tttgaactat taaatcaatt agttgaaagc aatgaaaaag
     3721 aattgaatga ttgtaatctt tctggcaaga gagataaaag acgagcaaaa atattagcat
     3781 caaaaaatta tattctctgg aggatggtaa gactggtaaa taggaaaaag aataaacgtt
     3841 agatcttgca ttaaataatc cgtcctcgta gcataggttg aaactatatg tcattttgtt
     3901 ggaatgaaat taattctggt ataaagtctt taattctcat attatgtatt ttttctttga
     3961 tgactttgtc tttatgggat gatgttgcaa caaagtttct tcatgcagct ggaattatat
     4021 ctgcattgta ttttctaata acaccaaaaa aaacaataac taacaatcct actttgttaa
     4081 ttttcatctc attatgtctt ttgggaatca taaatatcat ctggtattca cattataaag
     4141 tttcaggctc tgtttatacc aatgcatatc gtggcccaat ggaaactggg aaaattgcct
     4201 tgtgtagtgc ttttattttc ttggttcttt ttgctaaaaa tgagttgaga acaaaaataa
     4261 aatttgggaa actaattctg ttcgcatccc tggcaacgca gttacttttt tttgcgcatg
     4321 ccatgtggca acatttctat ttaaacgttg accgtgttgc attatcagct tcccacgcta
     4381 caacagcagg ctacatcatc cttttccctt ctttactggc atcaattctc attttaaaat
     4441 ccgactttag acataaaaca acattatata caattaactt catgcttagc ttatgtgctg
     4501 tcatagtaac tgaaacgcgt gcagccatat tagtgtttcc attctttgcg ttactattaa
     4561 tcgtaatgga tagttatatt aataagcgaa ttaattataa gttatattgt tttattgcga
     4621 ttgcattatt agcaggtgta ttttctttta aagatacatt gcttatgaga atgaataact
     4681 taaataacga tttagttaat tattcgcatg ataacaccag aacttcagtc ggtgcccgtc
     4741 tggcaatgta tgaagttggc ttaaaaacat attctccaat aggacaatca ctggaaaaac
     4801 gagcggaaaa aatacatgaa ctagaagaaa aagagcctag attgagtggc gctttaccct
     4861 atatagattc tcatttgcat aacgatctca tagatacgtt atcaacgcgt ggtattcctg
     4921 gagttgtatt aacaatttta gccttttcag caatactcat atatgcctta agaactgcta
     4981 aagaacctta tattttaatc ttgctttttt cactactggt agtaggccta agtgatgtaa
     5041 tactcttttc taaaccggtt ccgactgctg tgtttgtcac cataatattg ctttgtgctt
     5101 attttaaagc acaatcagac caatgtttat tagataaata atgaatatgt gaaataaaat
     5161 cagcaaggag gctgatttta tttcaaaatt atctattaag gggttgtaat ttaaatcata
     5221 gcaacgtttt cttcaatgaa tcgcttaaca gcatttgctg tcaattgaga caactcattc
     5281 cccggagccc tacaaaccat ctgattcttc ccatatccac caattaatcc cggatcggtt
     5341 ggtccataaa ccgtgatatt gggtctatcc agtgccgccg ttaaatggct taaccccgta
     5401 tccaccgaca ctacaaattt agccccggcc agcacgcggg caacgccttc cagactcatc
     5461 ttcggcaata cttcaacata agcaaatcct tccgccagtc gtttcgcccg ctcttcctca
     5521 tgcggcgcgc cccacggaag tttaatccgt attcctgaat cagccagtaa accaatcaat
     5581 tctcgccagt gttcttccgg ccagtgttta tcatcacggg tcgtcgcatg aagaaatacg
     5641 gcatattcgc cagcatctgt aggcaggttc gtcaggaaat gctgtgcaat agcataatcg
     5701 ccctgggttt gcggtttgct atagcccaga cttttggcaa acagttcgcg ggtgcgttct
     5761 acggcgtgct gctgttttgc aatatgatgc ttacgattgt aaaacagact ggctaaaggt
     5821 tcgcgagcgg tttgccagtc catgccatgc tttacgccat gcgccagacg cgtcaccagc
     5881 gccgcgcttt ttaccagccc ctgagcgtcg ataactgcgt catagttctc tgcttgtagc
     5941 gcttcacgaa acgctttgcg ttccgctttt atgggggccg agaaccaggc tttacgccag
     6001 cgacgtattg ccacaggaat aactcgctca acggcggcgt gccaggaagg aatctgtgcg
     6061 aacccttctt ccaccaccca gtcaaactta atccctggga ttgcctgctg ggcatcagtg
     6121 agtgcgggca acgtatggag aacatcgccc atcgacgatg ttttaacgat caaaacccgc
     6181 atccgtcagg cttcctcttg taacaatagc gcgttgagtt cttccagtac gcgctgggga
     6241 gtaatgtcga ttaagctctg gtgataaccc tccgcagcgt cacctttacg cactttgtga
     6301 tagccggtaa tcaggcggat cacgcgcgct ttatgggata gcggcggtgt gaagtccggg
     6361 ctactcggac catacagggc aaccagcgga cgattgagcg ccgccgcaac gtgcatcagg
     6421 ccagaatcgt tagtgacaat ggctttacag gctgcaatca ggataaccgc ttgatcaagc
     6481 tgtgtttccc ccgccaggtt ccgacaccat gcctgctgct cggtattcaa agcggcaaga
     6541 atctcattgc ccgcttcatg atctttcgcc gagccaaaca gaaccacctg ataaccttca
     6601 tcaatcagct gctttgccag ttccgcatag tggtagtgtg gccagcgttt tgccggacca
     6661 aactccgcac ccgggcaaaa accaatcatc ggacgttctg atgaaagcga aaattgatta
     6721 caggtatatg atttttcacc ttcgctcacc tgcaattgcg gccataacaa cggttttggt
     6781 agatcttgtg cggtacgcat cacgcctttg tcataggcca gcgcgacata gcgttccacc
     6841 attagcggcc aggcttcttt atcgagcacg cgtaaatcgt tgagtaaacc gtagcgcatc
     6901 tcgccgcgcc agccggtgcg atgaggaata cccgcgaaga aaggcactaa tgcagatttg
     6961 aaggagttgg gtaagacgta ggcgcggtcg tagcgctttt cacgcaggct atgacccagt
     7021 ttgcggcgtt cgccgatttc cagcgctccg tgaccgagag gcataggaat agcttcgtta
     7081 acttccggca tccgcgataa taatggacgg caccatgccg gtgccatcac atcgattatc
     7141 gcctggggat agcgcgcctg gagcgtgcga tagagacttt gcgacatcat catgtcgcca
     7201 acccaagacg ggccgatcac cagtattttc atgcagagct cttatgcgtc gcgattcagc
     7261 caggccatgt attccgttac accttcagca acggttttga acggtttgtc gtaacccgcc
     7321 gcgcgcagat ttgtcagatc tgcctgagtg aacgcctggt agcggccttt cagcttatcc
     7381 gggaacggaa tgtattcgat ctggcctttc ttgtgataag ccagcgtagc atcagctaca
     7441 gcctggaagg attccgcacg accagtaccg aggttgaaga tgccggaaac gccattttcc
     7501 aggaaccaca gattcacatc agccacgtcg cccacataga cgaagtcgcg tttgaagttc
     7561 tcgctacctt caaacagctt cggtgattca ccgttgttaa gctgggtgtt gagatggaaa
     7621 gcgacgctcg ccatgctgcc tttatggcct tcacgcggtc cataaacgtt gaaatagcgg
     7681 aagccaacaa tctgcgagtt agcttcaggc aggatttggc gaacatattc atcaaacagg
     7741 aattttgagt aaccgtagac gttcaacggt ttttcgtact cgcgggattc aataaagtcg
     7801 gaggtgcgtc cgccgtaggt ggctgcggaa gaggcataca ggaacgggat ttcgcgctcc
     7861 agacagtagt gcagcagctc tttggagtat tgatagttgt tatccatcat atacttgcca
     7921 tcccactcgg tggtggaaga gcacgcgcct tcgtggaaaa tcgcttcgac atcgccgaac
     7981 tcttcgccag ccataatctg gatcaggaag tcttccttat ccatatagtc cgcgatatcc
     8041 agatccacca ggttcacaaa cttggtgccg tctttcaggt tgtccaccac cagaatatcg
     8101 gtgatgcctt tatcattcag ggccttaacg atgttgctgc cgataaagcc cgcgccgccg
     8161 gtaacgatga tcataactgt aaccttcgaa ttatggagtc agagacaatc tcagacacga
     8221 atacttctat catatcacta cacggcgttg gcttcagcca ttcgccaaaa gtgagattga
     8281 tggacgttat ttatcctgaa ttttgcagaa gtgttaacgc gtcatctcgt cgcgacctat
     8341 aagtttgggt aatatgtgct ggaatttgcc ctgtctggag aatcgcaatg cgtggagaat
     8401 tttatcagca gttaaccaac gatctggaaa ccgcacgggc ggaagggttg tttaaagaag
     8461 agcgcattat tacgtctgcg cagcaagcag atatcactgt ggctgatgga agccacgtca
     8521 ttaacttttg tgccaataac tatctcgggc tggcgaatta tccggatctg attgcggcgg
     8581 caaaggcggg aatggattct cacggtttcg gcatggcttc ggtgcgtttt atttgcggca
     8641 ctcaggacag ccataaagag cttgaacaaa aactggcggc attcctgggg atggaagatg
     8701 cgattctcta ctcttcctgc tttgatgcta acggtggcct gtttgaaacg ctgctgggcg
     8761 cggaagacgc cattatctcc gacgcactga accacgcgtc tattattgat ggtgtgcgtc
     8821 tgtgtaaagc taaacgctat cgctatgcca acaacgatat gcaggagctg gaagcacgtc
     8881 tgaaagaagc gcgtgaagcc ggtgcgcgtc atgtgctgat tgccaccgat ggtgtgttct
     8941 cgatggacgg cgtgattgcc aacctgaagg gcgtttgcga tctcgcagat aaatatgatg
     9001 ccctggtgat ggtagacgac tcccacgcgg tcggttttgt cggtgaaaat ggtcgtggtt
     9061 cccatgaata ctgcgatgtg atgggccggg tcgatattat caccggcacg ctcggtaaag
     9121 cgctgggcgg ggcttctggt ggttataccg cggcgcgcaa agaagtggtt gagtggctgc
     9181 gccagcgttc tcgtccgtac ctgttttcca actcgctggc accggccatt gttgccgcat
     9241 ccatcaaagt actggagatg gtcgaagcgg gtagcgagct gcgtgaccgt ctgtgggcga
     9301 acgcgcgtca gttccgtgag caaatgtcgg cggcgggctt taccctggcg ggagccgatc
     9361 acgccattat tccggtcatg cttggtgatg cggtagtggc gcagaaattt gcccgtgagc
     9421 tgcaaaaaga gggcatttac gtcaccggtt tcttctatcc ggtcgttccg aaaggtcagg
     9481 cgcgtattcg tacccagatg tctgcggcgc atacccctga gcaaattacg cgtgcagtag
     9541 aagcattcac gcgtattggt aaacaactgg gcgttattgc ctgaggatgt gagatgaaag
     9601 cgttatccaa actgaaagcg gaagagggca tctggatgac cgacgttcct gtaccggaac
     9661 tcgggcataa cgatctgctg attaaaatcc gtaaaacagc catctgcggg actgacgttc
     9721 acatctataa ctgggatgag tggtcgcaaa aaaccatccc ggtgccgatg gtcgtgggcc
     9781 atgaatatgt cggtgaagtg gtaggtattg gtcaggaagt gaagggcttc aagattggcg
     9841 atcgcgtttc tggcgaaggc catatcacct gcggtcactg tcgcaactgt cgtggcggtc
     9901 gtacccatct gtgccgcaac acgatcggcg tcggcgttaa ccgcccgggc tgcttcgccg
     9961 aatatctggt gattccggcg ttcaacgcct tcaaaattcc cgacaatatc tccgacgacc
    10021 tggcttccat ttttgatccc ttcggtaacg ccgtgcatac cgcgctgtcg ttcgatctgg
    10081 ttggcgagga tgtgctggtc tctggtgcag gcccgattgg tattatggca gcggcggtgg
    10141 cgaaacacgt tggtgcacgc aatgtggtga tcactgatgt taacgaatat cgtctcgaac
    10201 tggcgcgcaa aatgggtatc acccgtgcgg tcaacgtcgc taaagaaaac cttaatgatg
    10261 tgatggcaga actgggcatg gccgaaggct ttgatatcgg tctggaaatg tccggtgcgc
    10321 cgccagcgtt tcgtaccatg ctcgacacca tgaaccacgg cggtcgcatt gcgatgctgg
    10381 gtattccacc gtccgatatg tctatcgact ggaccaaagt aatctttaaa ggcttgttca
    10441 ttaaaggtat ttacggtcgt gagatgtttg aaacctggta caagatggcg gcgctgattc
    10501 agtcaggtct ggatctctcg ccgatcatta cccatcgttt ctctatcgat gatttccaga
    10561 agggctttga cgctatgcgt tcgggccagt ccgggaaagt aattctgagc tgggattaac
    10621 acgaacaagg gctggtattc cagccctttt gcctgaggat aatccgttaa atatgtaaaa
    10681 tcctgtcagt gtaataaaga tatcgtaatt gcgctgatct cttatatagc tgctctcatt
    10741 atctctctac cctgaagtga ctctctcacc tgtaaaaata atatctcaca ggcttaatag
    10801 tttcttaata caaagcctgt aaaacgtcag gataacttca gaggtcgtcg gtaatttatg
    10861 atgaacagca ccaataaact tagtgttatt attccgttat ataatgcggg cgatgatttc
    10921 cgcacttgta tggaatcttt aattacgcaa acctggactg ctctggaaat cattattatt
    10981 aacgatggtt caacggataa ttctgttgaa atagcaaagc attacgcaga aaactatccg
    11041 cacgttcgtt tgttgcatca ggcgaatgct ggcgcatcgg tggcgcgtaa tcgtgggatt
    11101 gaagtggcaa cgggcaaata tgtcgctttt gtcgatgctg acgatgaagt ctatcccacc
    11161 atgtacgaaa cgctgatgac catggcgtta gaggacgacc tcgacgtggc gcagtgcaac
    11221 gctgactggt gttttcgtga aacgggagaa acctggaaat ccatccccag cgatcgcctt
    11281 cgctcaaccg gcgtattaac cggcccggac tggctgcgga tggggctttc ttcgcgccgt
    11341 tggactcacg ttgtctggat gggggtttat cgccgcgatg ttattgttaa aaataacatt
    11401 aaatttattg ccggattaca tcatcaggat attgtctgga caacagaatt tatgtttaat
    11461 gcgctgcgtg cgcgatatac cgagcaatcg ttatataaat attatctgca taatacgtca
    11521 gtgagtcggt tgcatagaca agggaacaaa aaccttaatt atcaacgtca ctatattaag
    11581 attactcgtc tgctagagaa attaaatcga aattatgccg acaaaattac gatttatccg
    11641 gaatttcatc agcaaataac ttacgaagca ttgcgtgttt gccatgcggt gcgcaaagag
    11701 ccggatatta ttacccgcca acggatgatt gccgagatat ttacttccgg tat
//
LOCUS       DADMKF010000125        11596 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.127, whole genome shotgun sequence.
ACCESSION   DADMKF010000125 DADMKF010000000
VERSION     DADMKF010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11596
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.127"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            203..874
                     /locus_tag="JNC24_003089"
     CDS             203..874
                     /locus_tag="JNC24_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HAY9159778.1"
                     /translation="MDKVLDSALLSSANKRKGILAIGAHPDDIELGCGASLARLAQKG
                     IYIAAVVMTTGNSGTDGIIDRHEESRNALKILGCNQTIHLNFADTRAHLQLNDMISAL
                     EDIIKNQIPSDVEIMRVYTMHDADRHQDHLAVYQASMVACRTIPQILGYETPSTWLSF
                     MPQVFESVKEEYFTVKLAALKKHKSQERRDYMRHDRLRAVAQFRGQQVNSDLGEGFVI
                     HKMIL"
     gene            884..2080
                     /locus_tag="JNC24_003090"
                     /pseudo
     CDS             884..2080
                     /locus_tag="JNC24_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414897.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
     gene            2104..2640
                     /locus_tag="JNC24_003091"
     CDS             2104..2640
                     /locus_tag="JNC24_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001351282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAY9159779.1"
                     /translation="MADQVIIDETAGEVVIGANTRICHGAVIQGPVVIGANCLIGNYA
                     FIRPGTIISNGVKIGFATEIKNAVIEAEATIGPQCYIADSVVANQAYLGAQVRTSNHR
                     LDEQPVSVRTPEGIIATGCDKLGCYIGQRSRLGVQVIILPGRIISPNTQLGPRVIVER
                     NLPTGTYSLRQELIRTGD"
     gene            2642..3415
                     /gene="yaiO"
                     /locus_tag="JNC24_003092"
     CDS             2642..3415
                     /gene="yaiO"
                     /locus_tag="JNC24_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiO family outer membrane beta-barrel protein"
                     /protein_id="HAY9159780.1"
                     /translation="MIKRTLLAAAIFSALPAYAGLTSITAGYDFTDYSGDHGNRNLAY
                     AELVAKVENATLLFNLSQGRRDYETEHFNATRGQGAVWYKWNNWLTTRTGIAFADNTP
                     VFARQDFRQDINLALLPKTLFTTGYRYTKYYDDVEVDAWQGGVSLYTGPVITSYRYTH
                     YDSSDAGGSYSNMISVRLNDPRGTGYTQLWLSRGTGAYTYDWTPETRYGSMKSVSLQR
                     IQPLTEQLNLGLTAGKVWYDTPTDDYNGLQLAAHLTWKF"
     gene            3603..3878
                     /gene="frmR"
                     /locus_tag="JNC24_003093"
     CDS             3603..3878
                     /gene="frmR"
                     /locus_tag="JNC24_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-responsive transcriptional
                     repressor FrmR"
                     /protein_id="HAY9159781.1"
                     /translation="MPSTPEEKKKVLTRVRRIRGQIDALERSLEGDAECRAILQQIAA
                     VRGAANGLMAEVLESHIRETFDRNDCYSREVSQSVDDTIELVRAYLK"
     gene            3913..5022
                     /locus_tag="JNC24_003094"
     CDS             3913..5022
                     /locus_tag="JNC24_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase"
                     /protein_id="HAY9159782.1"
                     /translation="MKSRAAVAFAPGKPLEIVEIDVAPPKKGEVLIKVTHTGVCHTDA
                     FTLSGDDPEGVFPVVLGHEGAGVVVEVGEGVTSVKPGDHVIPLYTAECGECEFCRSGK
                     TNLCVAVRETQGKGLMPDGTTRFSYNGQPLYHYMGCSTFSEYTVVAEVSLAKINPEAN
                     HEHVCLLGCGVTTGIGAVHNTAKVQPGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPKKFDLARRFGATDCINPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRAALES
                     AHRGWGQSVIIGVAGSGQEISTRPFQLVTGRVWKGSAFGGVKGRSQLPGMVEDAMKGD
                     IDLEPFVTHTMSLDEINDAFDLMHEGKSIRTVIRY"
     gene            5115..5948
                     /locus_tag="JNC24_003095"
     CDS             5115..5948
                     /locus_tag="JNC24_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase FrmB"
                     /protein_id="HAY9159783.1"
                     /translation="MELIEKHASFGGWQNVYRHYSQSLKCEMNVGVYLPPKAANEKLP
                     VLYWLSGLTCNEQNFITKSGMQRYAAEHNIIVVAPDTSPRGSHVADADRYDLGQGAGF
                     YLNATQAPWNEHYKMYDYIRNELPDLVMQHFPATTRKSISGHSMGGLGALVLALRNPD
                     EYVSVSAFSPIVSPSQVPWGQQAFAAYLGENKDAWLDYDPVSLISQGQRVAEIMVDQG
                     LSDDFYAEQLRTPNLEKICQEMNIKTLIRYHEGYDHSYYFVSSFIGEHIAYHANKLNM
                     R"
     gene            complement(6174..6713)
                     /locus_tag="JNC24_003096"
     CDS             complement(6174..6713)
                     /locus_tag="JNC24_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2058 domain-containing protein"
                     /protein_id="HAY9159784.1"
                     /translation="MAKLTLQEQLLKAGLVTSKKAAKVERTAKKSRVQAREARAAVEE
                     NKKAQLERDKQLSEQQKQAALAKEYKAQVKQLIEMNRITIANGDIGFNFTDGNLIKKI
                     FVDKLTQAQLINGRLAIARLLVDNNSEGEYTIIPASVADKIAQRDASSIVLHSALSAE
                     EQDEDDPYADFKVPDDLMW"
     gene            complement(6815..8026)
                     /gene="mhpT"
                     /locus_tag="JNC24_003097"
     CDS             complement(6815..8026)
                     /gene="mhpT"
                     /locus_tag="JNC24_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-(3-hydroxy-phenyl)propionate transporter MhpT"
                     /protein_id="HAY9159785.1"
                     /translation="MSTRTPSSSSSRLMLTIGLCFLVALMEGLDLQAAGIAAGGIAQA
                     FALDKMQMGWIFSAGILGLLPGALVGGMLADRYGRKRILIGSVALFGLFSLATAIAWD
                     FPSLVFARLMTGVGLGAALPNLIALTSEAAGPRFRGTAVSLMYCGVPIGAALAATLGF
                     AGANLAWQTVFWVGGVVPLILVPLLMRWLPESAVFAGEKQAAPPLRALFAPETATATL
                     LLWLCYFFTLLVVYMLINWLPLLLVEQGFQPSQAAGVMFALQMGAASGTLMLGALMDK
                     LRPVTMSLLIYSGMLASLLALGTVSSFNGMLLAGFVAGLFATGGQSVLYALALLFYSS
                     QIRATGVGTAVAVGRLGAMSGPLLAGKMLALGTGTVGVMAASAPGILVAGLAVFILMS
                     RRSRMQPCADA"
     gene            complement(8313..9326)
                     /gene="dmpG"
                     /locus_tag="JNC24_003098"
     CDS             complement(8313..9326)
                     /gene="dmpG"
                     /locus_tag="JNC24_003098"
                     /EC_number="4.1.3.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
                     /protein_id="HAY9159786.1"
                     /translation="MNGKKLYISDVTLRDGMHAIRHQYSLENVRQIAKALDDARVDSI
                     EVAHGDGLQGSSFNYGFGAHSDLEWIEAAADVVRHAKIATLLLPGIGTIHDLKNAWQA
                     GARVVRVATHCTEADVSAQHIQYARELGMDTVGFLMMSHMTTPENLAKQAKLMEGYGA
                     TCIYVVDSGGAMNMSDIRDRFRALKAVLKPETQTGMHAHHNLSLGVANSIAAVEEGCD
                     RIDASLAGMGAGAGNAPLEVFIAAADKLGWQHGTDLYALMDAADDLVRPLQDRPVRVD
                     RETLALGYAGVYSSFLRHCETAAARYGLSAVDILVELGKRRMVGGQEDMIVDVALDLR
                     NNK"
     gene            complement(9323..10273)
                     /locus_tag="JNC24_003099"
     CDS             complement(9323..10273)
                     /locus_tag="JNC24_003099"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase (acetylating)"
                     /protein_id="HAY9159787.1"
                     /translation="MSKRKVAIIGSGNIGTDLMIKILRHGQHLEMAVMVGIDPQSDGL
                     ARARRMGVATTHEGVIGLMNMPEFADIDIVFDATSAGAHVKNDAALREAKPDIRLIDL
                     TPAAIGPYCVPVVNLEANVDQLNVNMVTCGGQATIPMVAAVSRVARVHYAEIIASIAS
                     KSAGPGTRANIDEFTETTSRAIEVVGGAAKGKAIIVLNPAEPPLMMRDTVYVLSDEAS
                     QDDIEASINEMAEAVQAYVPGYRLKQRVQFEVIPQDKPVNLPGVGQFSGLKTAVWLEV
                     EGAAHYLPAYAGNLDIMTSSALATAEKMAQSLARKAGEAA"
     gene            complement(10270..11079)
                     /gene="mhpD"
                     /locus_tag="JNC24_003100"
     CDS             complement(10270..11079)
                     /gene="mhpD"
                     /locus_tag="JNC24_003100"
                     /EC_number="4.2.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-4-pentenoate hydratase"
                     /protein_id="HAY9159788.1"
                     /translation="MTKHTLEQLAADLRRAAEQGEAIAPLRDLIGIDHAEAAYAIQHI
                     NVQYDVVQGRRVVGRKVGLTHPKVQQQLGVDQPDFGTLFADMCYGDNEIIPFSRVLQP
                     RIEAEIALVLNRDLPATDITFDELYNAIEWVLPALEVVGSRIRDWSIQFVDTVADNAS
                     CGVYVIGGPAQRPAGLDLKNCAMKMTRNNEEVSSGRGSECLGHPLNAAVWLARKMASL
                     GEPLRAGDIILTGALGPMVAVNAGDRFEAHIEGIGSVAATFSSAAPKGSLS"
     gene            complement(11089..>11596)
                     /locus_tag="JNC24_003101"
     CDS             complement(11089..>11596)
                     /locus_tag="JNC24_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414883.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAY9159789.1"
                     /translation="VAFTLNWPERVGKLVLMGGGTGGMSLFTPMPTEGIKRLNQLYRQ
                     PTIENLKLMMDIFVFDTSDLTDALFEARLNNMLSRRDHLENFVKSLEANPKQFPDFGP
                     RLAEIKAQTLIVWGRNDRFVPMDAGLRLLSGIAGSELHIFRDCGHWAQWEHADAFNQL
                     VLNFLARP"
BASE COUNT         2799 a         3128 c         2887 g         2782 t
ORIGIN      
        1 tatacatacc aaacatacaa acacttaaca tatgaattgc ataaaaacat tacccagtcg
       61 aacctccggg tctgaaagcc cttgcttttc agttattagc aacatttaac atttataaat
      121 taacatctgg ctgcgccaga gattacgtct ctgagtgcat cgttgtgctt gtagtataat
      181 aaataaaatg gagtgtcatt aagtggataa ggttttagat tcagccctcc tttcttcggc
      241 aaataaaaga aagggtatcc ttgctattgg cgcacatcct gacgatatag aattaggctg
      301 tggcgcatcg cttgctcgtc ttgcgcaaaa aggaatttat atcgcagcag tggtgatgac
      361 taccggcaac tctggtacag atggaataat cgatcgccat gaagaatcgc gcaacgccct
      421 aaagatatta gggtgcaacc aaactattca tcttaatttt gctgacaccc gcgctcattt
      481 acagctcaat gatatgattt ccgccctgga agacatcatt aaaaatcaaa ttccttctga
      541 tgttgaaatc atgcgggtat ataccatgca tgatgccgac cgccatcagg atcatctcgc
      601 tgtttatcaa gcttcaatgg ttgcctgccg cactattcca caaattctcg gctacgaaac
      661 cccaagtacc tggctttcat ttatgcctca ggtttttgaa tccgttaaag aagaatattt
      721 cacagtcaaa cttgctgcat taaaaaaaca taaaagccag gaacgacgtg attatatgcg
      781 tcatgatcgc ctgcgtgcag ttgcacaatt tcgcgggcaa caggtcaata gcgatctggg
      841 tgaaggcttt gtaattcata aaatgattct ttgagcaaat tgcatgaaaa cctggatatt
      901 tatctgtatg tccatagcaa tgttgctatg gtttttaagt acgctaagac gtaaacccag
      961 tcaaaagaaa ggctgtattg acgccattat acctgcgtat aacgaaggcc cgtgtctggc
     1021 gcagtcactg gataatctac tgcgtaaccc ttatttttgc cgggtaattt gcgttaacga
     1081 cggctccacg gacaataccg aagcggtcat ggcggaagtc aaacgcaaat ggggcgaccg
     1141 ctttgttgcc gtcacgtaaa aaaataccgg taaaggtggt gcgctgatga atggcctcaa
     1201 ttatgccacc tgcgaccagg tttttttaag tgatgccgac acctatgttc cgcccgatca
     1261 agacggaatg ggctatatgc tggcagaaat taagcgcggt gctgatgccg taggcggcat
     1321 tccctctact gcgttgaaag gcgcggggct gttaccgcac atccgcgcga ccgtaaagtt
     1381 gccgatgatt gttatgaagc gcacgctaca gcagctcctg ggcggcgcac cgtttattat
     1441 cagcggtgcc tgcggaatgt tccgtactga tgtattgcgt aagttcggtt tctcggatcg
     1501 tactaaagtc gaagaccttg atctcacctg gacattagtg gcaaacggct accgtattcg
     1561 gcaggcgaat cgctgcatcg tatacccaca ggaatgcaac agtccgcgtg aggagtggcg
     1621 tcgctggcgg cgttggattg tgggctacgc ggtctgtatg cgcctgcata aaaggctttt
     1681 atttagccgc ttcggtatct tcagtatatt tcctatgctg ttggttgtga tttatggcgt
     1741 tgggatttat ctcactacct ggtttaatga attcatcacc accgggccgc atggtgtggt
     1801 gttggcaatg tttccgctta tctggatcgg cgtagtttgt gttattggtg cttttagcgc
     1861 ctggtttcat cgttgctggt tgttggtgcc tttagcgccg ctttccgttg tgtatgtatt
     1921 attagcttat gccatctgga ttatctatgg acttattgcc ttttttactg gacgcgaacc
     1981 tcagcgcgac aaacccaccc gctattccgc actggtggaa gcgtcaaccg cttattccca
     2041 accttctgtc acaggaactg aaaaactatc tgaagcttaa tgttaaagag aaaaattatt
     2101 cagattgcag accaggtaat tattgatgaa actgcaggtg aagttgttat cggcgcgaat
     2161 acccgtattt gtcatggtgc cgttattcag ggtccggtag tgattggcgc aaactgcctg
     2221 ataggtaatt atgcgtttat tcgtcctggc acaataatca gcaatggtgt aaaaattggt
     2281 tttgccaccg aaattaaaaa tgcggttatt gaagcggaag caacgattgg tccgcaatgt
     2341 tatattgccg actcggtagt tgcaaaccag gcatatttgg gcgcacaagt acgtaccagt
     2401 aatcatcgtc tggatgaaca gcctgtgtct gttcgaactc cagagggaat tatcgctacc
     2461 ggatgcgata aattaggttg ttatatcggg cagcgttcac gccttggcgt acaagttatt
     2521 attttgcctg ggcgaattat ttccccgaac acgcaacttg gcccgcgcgt gattgtagaa
     2581 cgtaatttac ctaccggaac ttattcactc cgacaagaac ttatccgtac aggagattaa
     2641 aatgataaaa cggacgttat tagcggcggc catttttagc gcattgcccg cttatgccgg
     2701 gttaacttcc attaccgcgg gctacgattt taccgattat tctggcgatc atggcaaccg
     2761 taatttagcg tatgctgaac tggtggcgaa agttgaaaac gcaacgctgc tttttaatct
     2821 ttcccagggg cgtcgtgatt atgaaactga acatttcaat gccactcgcg gtcagggtgc
     2881 ggtctggtat aaatggaata actggctgac aacccgaacg ggtattgcct ttgcggataa
     2941 tacgccggtc tttgcccgcc aggattttcg tcaggatatt aacctggccc tgttgccaaa
     3001 aacgcttttc acgaccggtt atcgctacac taaatattac gatgatgtcg aagtcgatgc
     3061 ctggcaaggc ggcgtatcac tctatactgg cccggtgatc accagctacc gctataccca
     3121 ttatgactcc agcgatgcag gtggtagtta tagcaatatg atttccgtgc gtctgaatga
     3181 cccgcgcggc actggttata cgcaactatg gctaagccgc ggaacaggcg cttacaccta
     3241 tgactggacg ccagaaacac gctacggcag catgaagagc gtcagtttgc aacgtattca
     3301 accgctgact gagcaactta atctcgggct gacggcaggt aaagtgtggt acgacacccc
     3361 aaccgatgat tataacggtc tgcaacttgc agcccatctg acctggaaat tctgattgct
     3421 tctgccgccc gctatccggg gcggccttcc ctgccgatta gccccactcc ctttcctcgt
     3481 tgttttccga ccacattcac cggataaatt ttattctcca gcgttatata ctatagggag
     3541 gtatgcattg acatatagca tacctcccta tagtatattg cgtgcagatg atgaggtgcg
     3601 aaatgcccag tactccggaa gagaagaaaa aggtccttac tcgagttcgt cgtattcggg
     3661 ggcagattga tgctctggaa cggtcgctgg agggtgatgc cgaatgccgt gccatactcc
     3721 aacagatcgc tgccgttcgg ggcgcggcta acgggctgat ggcagaagtg cttgaaagcc
     3781 atatccggga aacgtttgac cgaaatgact gctacagccg cgaagtcagc caatccgttg
     3841 acgacactat tgaactggtt cgagcctatc ttaaatagct gaatctatta ccatattgag
     3901 gaagagcgag agatgaaatc acgtgctgcc gttgcatttg ctcccggtaa accgctggaa
     3961 atcgttgaaa ttgacgttgc accaccgaaa aaaggtgaag tgctgattaa agtcacccat
     4021 accggcgttt gccataccga cgcatttacc ctctccgggg atgacccgga aggtgtattc
     4081 ccggtggttc tcggtcacga aggggccggc gttgtggttg aagtcggtga aggcgtaacc
     4141 agcgtcaaac ctggcgacca tgtgatcccg ctttacaccg cggagtgcgg cgagtgtgag
     4201 ttctgtcgtt ctggcaaaac taacctctgt gttgcggttc gcgaaaccca gggtaaaggc
     4261 ttgatgccag acggcaccac ccgtttttct tacaacgggc agccgcttta tcactacatg
     4321 gggtgctcta cattcagtga atacaccgtg gtcgcggaag tgtctctggc caaaattaat
     4381 ccagaagcaa accatgaaca cgtctgcctg ctgggctgtg gcgtgaccac cggtattggc
     4441 gcggtgcaca acacagctaa agtccagcca ggtgattctg ttgccgtgtt tggtcttggc
     4501 gcgattggtc tggcagtggt tcagggcgcg cgtcaggcga aagcgggacg gattatcgct
     4561 atcgatacca acccgaagaa attcgatctg gcgcgtcgct tcggtgctac cgactgcatt
     4621 aacccgaatg actacgacaa accgattaaa gatgtcctgc tggatatcaa caaatggggt
     4681 atcgaccata cctttgaatg catcggtaac gtcaacgtga tgcgtgcggc gctggaaagt
     4741 gcgcaccgcg gctggggtca gtcggtgatc atcggggtgg caggttctgg tcaggaaatc
     4801 tccacccgtc cattccagtt ggtcactggt cgcgtatgga aaggttccgc gtttggcggc
     4861 gtgaaaggtc gttcccagtt accgggtatg gttgaagatg cgatgaaagg tgatatcgat
     4921 ctggaaccgt ttgtcacgca taccatgagc cttgatgaaa ttaatgacgc cttcgacctg
     4981 atgcatgaag gcaaatccat tcgaaccgta attcgttact gatttcccgc aggtataccc
     5041 cgtccacttc agacggggtt cttaatactc tccccgggca gccgtccggg ggattaaccg
     5101 tgagataatg actgatggaa ctcattgaaa aacatgccag ctttggcggc tggcaaaatg
     5161 tgtatcggca ttattcccaa tcactgaaat gtgaaatgaa tgtcggcgtc tatctcccac
     5221 caaaagccgc gaatgaaaaa ttgccggtgc tgtactggct ttcaggcctg acctgcaacg
     5281 agcagaattt cattactaaa tcggggatgc agcgttacgc ggctgagcac aacattattg
     5341 ttgttgcgcc ggacaccagt ccgcgaggca gtcatgtcgc agatgccgac cgttacgatc
     5401 tcgggcaagg tgccgggttt tacctgaacg caacgcaagc gccgtggaat gaacattaca
     5461 aaatgtatga ctatatccgc aacgagctgc cggatttagt gatgcaacat tttccggcaa
     5521 cgaccagaaa gtctatctct ggtcattcta tgggcgggct gggcgcgctg gtgctggcgt
     5581 tacgtaaccc agatgaatat gtcagcgtct cggcgttttc gcccattgtc tccccatcgc
     5641 aagtaccgtg gggacagcaa gcctttgccg catatcttgg tgaaaataaa gatgcctggt
     5701 tggattacga tccggtgagt cttatttcac aaggtcaacg cgttgcggaa atcatggttg
     5761 atcaggggtt gagtgatgat ttttacgcgg aacagctgcg gactccaaat cttgaaaaga
     5821 tctgccagga gatgaatatc aagacgttaa tccgttatca cgagggttac gaccacagct
     5881 attattttgt ctccagtttt attggcgagc atattgccta ccacgccaat aaactgaata
     5941 tgcgttgata atagtgcacg actgccggat gcggcgtgaa cgccttatcc ggcctacact
     6001 tcgcccgtaa accgtaggcc tgataagacg cgcaaagcgt cgcatcaggc atggcgcacg
     6061 actgccggat gcggcgtgaa cgccttatcc ggtctacact tcgcccataa accgtaggcc
     6121 tgataagacg cgcatagcgt cgcatcaggc atctgcgcac gacttatcta tgtttaccac
     6181 atcaaatcat caggcacttt gaagtcggca tacggatcgt cttcatcctg ctcttcggca
     6241 ctgagcgcgc tgtgcaacac aatactgctg gcatcacgct gagcaatttt atcggcaaca
     6301 ctggcgggaa taatggtgta ttcaccttcg ctattgttat cgaccaacaa gcgggcaatc
     6361 gccagacgac cattaatcaa ctgcgcctgt gtgagcttat cgacaaaaat cttcttaatc
     6421 agattgccgt cagtgaagtt aaaaccaata tcgccattgg caatggtgat tcggttcatt
     6481 tcaatgagct gcttcacctg ggctttatat tctttcgcca acgccgcttg tttttgctgt
     6541 tcgctaagct gtttatcacg ctcaagctgt gcctttttat tttcttctac cgcagcccga
     6601 gcttcacgcg cctgaacgcg cgattttttc gccgttctct cgaccttcgc cgcttttttg
     6661 ctggtgacta atcctgcttt gagcaactgc tcttgtaagg taagttttgc catctttgtt
     6721 tccaaacccg ctgaataatt tttagcgagt atacctgtaa acgccgtggg tgtattgggg
     6781 gcaagtccct ttcgcccctg cttaataagg cgcgtcaggc atcggcacac ggctgcattc
     6841 ttgatctccg actcatcaaa ataaacaccg ccaacccagc aacaagaata cccggtgcag
     6901 aagcggccat tacgccgacc gtgccagtgc ctaatgccag cattttcccg gccagtaacg
     6961 gaccgctcat agcccccaga cgccctaccg ccacggctgt tcccacacct gttgcgcgga
     7021 tctgcgaact gtaaaacaac agtgccaggg catacaaaac gctttgccca cctgtcgcaa
     7081 acaaccccgc gacaaatccc gccagcaaca taccgttaaa tgacgacacc gttccaagcg
     7141 ccagcagcga agctaacatg ccgctataaa tcagtagcga catggttact ggacgcagct
     7201 tatccatcaa tgcgcccaac attaacgtcc cgcttgccgc ccccatttgc agggcaaaca
     7261 tcacccctgc cgcctgcgat ggctggaatc cttgctccac caaaagtagc ggtagccagt
     7321 tgatcaacat ataaaccacc agcagagtga agaaataaca caaccacagc agcagcgtcg
     7381 cggttgccgt ttctggcgca aataaggcac gcagtggtgg cgcagcctgt ttttcgccag
     7441 cgaaaaccgc tgactccggc agccagcgca ttaatagcgg caccagaatc aacggcacca
     7501 caccacctac ccaaaacacc gtttgccatg ctaagtttgc ccccgcgaaa cccagtgtcg
     7561 ccgccagcgc cgcgccaatg ggaacaccgc aatacatcag gctcactgcc gtcccacgaa
     7621 aacgtggacc cgcggcttca gatgtcaggg cgataagatt cggcaacgcc gcccccagcc
     7681 cgacaccggt catcagccgc gcaaagacca gtgaggggaa atcccaggca atcgccgttg
     7741 ccagtgagaa caaaccaaac agcgcaactg agccaatcaa aatacgctta cgaccataac
     7801 ggtctgccag catcccgcca accaacgcgc cgggtagcaa accgagtatt ccggcgctaa
     7861 atatccagcc catttgcatt ttatcgagtg cgaaagcctg ggcgatgcca cccgccgcaa
     7921 tgccagccgc ctgaagatcc agcccttcca tcagagcgac caaaaaacaa agcccgatgg
     7981 tcagcatcag gcgggatgaa gatgatgaag gggtacgagt cgacataaga tgaacctcat
     8041 tgcgagtgca ggtgaatcgc gactgataca ttgccagccg cgtaatcaga actatttaat
     8101 gactgcctac gggcgaaatg tagcgaaatg taggccggat aaggcgttca cgccgcatcc
     8161 ggcagtcgtg cgcagatgcc tgatgcgacg ctaacgcgtc ttatcagacc tacaaattct
     8221 cacgccatcc gtaggccgga caaggcgttc acgccgcatc cggctgtcgt gctgggatgg
     8281 gcatacaaaa tgcacgttat cggcagtcat cattatttgt tgttgcgcag atccagcgcc
     8341 acgtcgacga tcatatcctc ctggccgcca accatccggc gtttgcccag ctcaacgaga
     8401 atatccaccg cacttaagcc ataacgcgcc gccgccgttt cacagtgacg caggaagctc
     8461 gagtaaacac cagcgtatcc cagcgccagc gtttcgcgat cgactcgtac cggacgatcc
     8521 tgcaacggac gcaccaggtc gtcggcggca tccattaacg catagagatc ggtcccatgc
     8581 tgccagccca gtttatccgc ggcggcaata aacacttcca gcggtgcgtt acctgcgccc
     8641 gcgcccattc ccgcgaggct ggcgtcgatt cggtcgcagc cctcttccac cgccgcgata
     8701 gagttcgcca cgccaagact caggttatgg tgagcgtgca tgccggtttg cgtttctggt
     8761 ttcaacactg ctttcagggc gcggaaacgg tcacggatat cgctcatgtt catcgcaccg
     8821 ccagaatcca ccacataaat acaggtcgca ccgtagcctt ccatcagctt tgcctgcttg
     8881 gcgagattct ccggcgtggt catatggctc atcatcagaa aaccaacggt gtccattccg
     8941 agctcgcggg catactgaat atgctgggcg gaaacatcag cttcggtaca gtgcgttgcc
     9001 acacgaacca cccgcgcgcc agcctgccag gcatttttca gatcgtgaat agtgccgatt
     9061 cctggcagca acaacgtcgc gattttggcg tgcctcacca catccgccgc cgcttcaatc
     9121 cattcaaggt cgctatgtgc gccgaaacca tagttaaagc tggaaccttg caaaccgtcg
     9181 ccgtgggcca cttcaatcga atccacgcgg gcatcgtcca gtgctttggc aatctggcga
     9241 acgttttcca gcgaatactg atgacgaatg gcgtgcatac cgtcacgcaa tgtgacgtcc
     9301 gagatataaa gttttttacc gttcatgccg cttctcctgc cttgcgcgcc agtgactggg
     9361 ccattttttc cgctgtcgcc agcgcactgg aagtcataat atcgaggttg cccgcatagg
     9421 caggcagata atgcgctgcg ccttcgactt ccagccagac cgctgttttc agtccggaga
     9481 attgccccac gcccggtaaa ttgaccggtt tatcctgcgg gataacttca aactgcacgc
     9541 gctgtttcag gcgataaccc ggtacgtaag cctgcaccgc ctcagccatt tcattgattg
     9601 aggcttcgat atcatcttgt gaagcttcgt cgctcaatac atacaccgtg tcacgcatca
     9661 tcaacggtgg ctctgctggg ttaagcacaa taatcgcctt cccttttgcc gcgccgccca
     9721 ccacttcaat ggctcgggaa gtggtttccg taaattcatc gatattggca cgcgtgccag
     9781 gtccggcaga tttactggcg atagaagcga taatttcggc gtaatgaaca cgcgccacgc
     9841 gtgaaactgc cgccaccatt ggaatggtgg cctggccgcc gcaggtgacc atgttgacgt
     9901 tcagttgatc gacgttcgcc tcgaggttaa ccaccggcac gcagtaaggg ccgatggcag
     9961 caggcgtcag gtcaattaag cgaatatccg gtttcgcttc gcgtaaagcg gcatcgtttt
    10021 tcacatgcgc gcccgcgctg gtcgcatcaa atacaatgtc gatatcagca aattcaggca
    10081 tgttcatcag cccgatcacc ccttcatggg tggtggcgac gcccatacgt ctggcacgcg
    10141 ccagaccgtc ggactgagga tcaatgccga ccatcaccgc catctccaga tgctgaccgt
    10201 gacgcaaaat tttaatcatc agatcggtgc caatgttgcc agaaccgata atggcgactt
    10261 tacgcttact catgacagac ttccttttgg ggctgcgctt gaaaatgtcg cagcaactga
    10321 acctatgcct tcaatatggg cttcaaaacg atcgcccgca ttcaccgcca ccatcgggcc
    10381 taatgccccg gtaagaatga tatctccggc gcgcagcggt tcacccagac tggccatttt
    10441 gcgtgccagc cagacggcgg cattaagcgg atgtcccagg cattcgctgc cgcgcccgct
    10501 ggaaacctct tcgttattac gcgtcatctt catggcgcag tttttcaggt ctaaccccgc
    10561 cggacgttgc gccggaccgc cgatgacata caccccgcag gaagcgttat ctgccacggt
    10621 atcgacaaac tgaatcgacc agtcgcgaat gcggctcccc accacttcca gcgccggaag
    10681 tacccattca atggcgttat acagttcgtc gaaggtgata tcagttgcgg gtaaatcgcg
    10741 gttcaacacc agtgcgatct ccgcttcaat gcggggttgc agaacacggg aaaaaggaat
    10801 gatttcgtta tcgccataac acatgtcggc aaataacgtc ccaaaatccg gttgatcaac
    10861 gcccagttgt tgttgcactt tcggatgcgt caggcccact ttacgcccta ccacgcgacg
    10921 cccctgcaca acgtcatatt gcacatttat gtgctgaatg gcgtaggccg cttcagcgtg
    10981 atcgatacca atcagatcgc gcagcggcgc aatcgcttcg ccctgctctg cggcgcggcg
    11041 taaatccgcc gccagttgct caagagtatg cttcgtcatg accattcctt aagggcgtgc
    11101 gaggaaattc agcaccaatt gattgaaagc gtcggcatgt tcccactgcg cccagtgacc
    11161 acagtcgcgg aagatatgca gttcagaacc ggcaatgccg gacagcagac gcagacccgc
    11221 atccatcggc acaaagcggt cgttgcgccc ccagacaatc agggtttgcg ctttgatttc
    11281 cgccagacgt gggccaaaat ccgggaactg tttcggatta gcttccaggc tcttaacgaa
    11341 gttttccagg tgatcgcggc gcgacagcat attattcagg cgcgcttcaa acagggcgtc
    11401 ggtcaaatcg ctggtatcga aaacgaagat atccatcatc agcttcaggt tttcgatagt
    11461 cggctgacga taaagctgat tcagtcgctt aataccttcg gttggcatcg gcgtaaacaa
    11521 actcatgccg cccgtaccgc cgcccatcag caccagtttg ccgacgcgct ccggccagtt
    11581 aagggtgaac gccaca
//
LOCUS       DADMKF010000126        11542 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.128, whole genome shotgun sequence.
ACCESSION   DADMKF010000126 DADMKF010000000
VERSION     DADMKF010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11542
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.128"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            460..963
                     /locus_tag="JNC24_003102"
     CDS             460..963
                     /locus_tag="JNC24_003102"
                     /inference="COORDINATES: protein motif:HMM:NF026057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY9159790.1"
                     /translation="MENWVIQELKSLDIGDSRLDKRVKHILNSLSRSPEESIPVSCRT
                     WSETKAAYRCFSNDKVSANKIMAPHKENITERAQQFKRVLVLQDTTELNYSGQKEKQG
                     VGHKKHKDERNLFLHPQLVISESGVCLGVYDDYQWFRDELKTSKNTRQEICNDLLHKK
                     HVSEKET"
     gene            complement(1336..2529)
                     /locus_tag="JNC24_003103"
     CDS             complement(1336..2529)
                     /locus_tag="JNC24_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY9159791.1"
                     /translation="MSPGIEDTPQKTLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPWWGKQISRTGSKPVVLWALGGYGISFTLLGLGSVLM
                     ATSAITTAVGLGILIIARIAYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLAIHPLAPLGLLMAAPVLALLMLLRLPGTPPQPTPECKSVSLKRDC
                     LPYLLCAILLAAAVSMMQLGLSPALTRQFVTDTTAISQQVAWLLGLSAVAALIAQFGV
                     LRPQRLTPVALLLSAGVLMSGGLAIMLSEQLWLFYPGCAVLSFGAALATPAYQLLLND
                     KLADGAGAGWLATSHTLGYGLCALLVPLVSKTGVAIALIMAALFATILFTIVSVFIWH
                     YRTIK"
     gene            2665..4389
                     /gene="iucA"
                     /locus_tag="JNC24_003104"
     CDS             2665..4389
                     /gene="iucA"
                     /locus_tag="JNC24_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HAY9159792.1"
                     /translation="MTLPSEKPATDVAAQCFLNALIRETTDWKLTEYPPDELLIPLDE
                     QKSLHFRVAYFSPTQHHRFAFPARLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLC
                     ETFHQRVLESHVHTQQAIDARHDWAALREKALNFGEAEQALLTGHAFHPAPKSHEPFN
                     RREAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
                     QWLFPLHPWQGEYLLQQGWCQALVAKGLIKDLGEAGTSWLPTTSSRSLYCATSRDMIK
                     FSLSVRLTNSIRTLSVKEVKRGMRLARLAQTDGWQMLQVRFPTFRVMQEDGWAGLLDL
                     NGNIMQESLFALRENLLVDQPKSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITV
                     QQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSA
                     FMPHATDWLDSIGEAQAENIFTHEQLLRYFPYYLLVNSTFAVTAALGAAGLDSESNLM
                     ARVRASLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFA
                     NPLQAQEV"
     gene            4390..5337
                     /gene="iucB"
                     /locus_tag="JNC24_003105"
     CDS             4390..5337
                     /gene="iucB"
                     /locus_tag="JNC24_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase"
                     /protein_id="HAY9159793.1"
                     /translation="MSEANIIHSRYGLRCEKLDKPLNLGWGLDNSAVLHCPGELPTGW
                     LCDALDQIFIAAPQLSAVALPWAEWREEPQALTLFGQVKSDIIHRTAFWQLPLWLSSP
                     ANRASGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQTAYLERQLTGKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGLTHYLLLDEPRTQRTVLEPRTDNQR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            5337..7079
                     /gene="iucC"
                     /locus_tag="JNC24_003106"
     CDS             5337..7079
                     /gene="iucC"
                     /locus_tag="JNC24_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucC"
                     /protein_id="HAY9159794.1"
                     /translation="MNHKDWDFVNRRLVAKMLSEMEYEQVFHAESQGDDHYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCTDEPVLAQTLLMQLKPVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLDADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     TNTFRLHWLAVKREHMIWRCDNDLDIQQLLTAAMDPQEFTRFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFVEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEAFPEMDSLPQEVRDATSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAAVLSDYMNKHPQMAERFALFSLFRPQIIRVVLNPVKLTWPDLDGGSRMLPNYLEN
                     LQNPLWLVTQEYES"
     gene            7076..8413
                     /gene="iucD"
                     /locus_tag="JNC24_003107"
     CDS             7076..8413
                     /gene="iucD"
                     /locus_tag="JNC24_003107"
                     /EC_number="1.14.13.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase"
                     /protein_id="HAY9159795.1"
                     /translation="MKKSVDFIGVGTGPFNLSIAALSHQIEELNCLFFDEHPHFSWHP
                     GMLVPDCHMQTVFLKDLVSAVAPTNPYSFVNYLVKHKKFYRFLTSRLRTVSREEFSDY
                     LRWAAEDMNNLYFSHTVENIDFDKKSRLFLVQTSRGEYFARNICLGTGKQPYLPPCVK
                     HVTQSCFHASEMNLRRPDLSGKRITVVGGGQSGADLFLNALRGEWGEAAEINWVSRRN
                     NFNALDEAAFADEYFTPEYISGFSGLKEDIRHQLLDEQKMTSDGITADSLLTIYRELY
                     HRFEVLRKPRNIRLLPSRSVTTLESSGPGWRLLMEHHLDQGRESLESDVVIFATGYRS
                     ALPQILPSLMPLITMHDKNTFKVRDDFTLEWSGPKENNIFAVNASMQTHGIAEPQLSL
                     MAWRSARILNRVLGRDLFDLSMPPALIQWRSGSRKKPQPEAASLTHYTANIQE"
     gene            8419..10614
                     /gene="iutA"
                     /locus_tag="JNC24_003108"
     CDS             8419..10614
                     /gene="iutA"
                     /locus_tag="JNC24_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HAY9159796.1"
                     /translation="MRKKYMPRALGPLLLVVLSPAVAQQNDDNEIIVSASRSNRTVAE
                     MAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSQSRTNYGMNMRGRPLVVLID
                     GVRLNSSRSDSRQLDSVDPFNIDHIEVISGATALYGGGSTGGLINIVTKKGQPETMME
                     FEAGTKSGFNSSKDHDERIAGAVSGGNDHISGRLSVAYQKFGGWFDGNGDATLLDNTQ
                     TGLQHSNRLDIMGTGTLNIDESRQLQLITQYYKSQGDDNYGLNLGKGFSAISGSSTPY
                     VSKGLNSDRIPGTERHLISLQYSDSDFLGQELVGQVYYRDESLRYYPFPTVNANKQAT
                     AFSSSQQDTDQYGMKLTLNSQLMDGWQITWGLDAEHERFTSNQMFFDLAQASASGGLN
                     NHKIYTTGRYPSYDITNLAAFLQSSYDINDIFTVSGGVRYQYTENRVDDFIDYTQQQK
                     IAAGKAISADAIPGGSVDYDNFLFNAGLLMHITERQQAWFNFSQGVALPDPGKYYGRG
                     IYGAAVNGHLSLTKSVNVSDSKLEGVKVDSYELGWRFTGDNLRTQIAAYYSLSNKSVE
                     RNKDLTISVKDDRRRIYGVEGAVDYLIPDTDWSTGVNFNVLKTESKVNGQWQKYDVKE
                     SSPSKATAYINWAPEPWSLRVQSTTSFDVSDAEGNDINGYTTVDFISSWQLPVGTLSF
                     SVENLFDRDYTTVWGQRAPLYYCPGYGPASLYDYKGRGRTFGLNYSVLF"
BASE COUNT         2927 a         2896 c         2949 g         2770 t
ORIGIN      
        1 ctcaacgaag gggccgacaa acacgttgtc cctgagttca cattcataaa tgttgaccgg
       61 tttgacaata cggacattga tcccataaat cacatcccgg acagcacatt ccagaaattt
      121 catactcaca cgtcctttaa atacagatga catttgccgg aattataccg cccagattca
      181 cttgccatgt ttcagaatag cttataagcc gcacttttac cgatactcag caacaaatca
      241 ggtctgtatc cacttcagcc ggaaaccgga tctgcgcggc ggggaacggg caagaaatat
      301 gataattaag caacacacat aaaatcatat tgcataatcg catctgaatc gaacacttat
      361 tcgcatcact atcgatcatc acttcgcatc tcataccctt acccacaagt tgaaataata
      421 gtattatatt gaaaagacag agattatttc agaggtgtta tggaaaactg ggttattcag
      481 gagctaaaat cactggatat aggggactca agacttgata aaagagtaaa acatatcctc
      541 aatagcctca gtcgttcacc agaagaaagt attcctgttt cctgccgtac ctggagtgaa
      601 acaaaggcag cttatcgttg tttttcaaac gataaagtct ctgctaataa aattatggct
      661 cctcacaaag aaaatatcac tgaaagagct cagcagttta aaagagtttt agttttacag
      721 gatacaacag aacttaatta ttctggtcag aaagaaaaac aaggagttgg tcacaaaaaa
      781 cataaagatg aaagaaactt atttcttcat cctcaactcg tcatcagtga gtcaggtgtt
      841 tgcctcggtg tttatgatga ctatcagtgg ttcagggatg aattaaaaac cagtaaaaat
      901 acccgacaag aaatctgcaa tgatttactt cataaaaaac atgtcagtga aaaagaaacc
      961 tagcgttggg ttgaaggata taataaagca acagaactgg ctcgccaatg ccctgatacc
     1021 catgtattgt caatttctga tcgtgaaggc gatttttatg acctgtttga acgcgcagag
     1081 cagactcccg gaataaaagc tgactggctt gtcagaatga aatttaataa cagagcaacg
     1141 ctgaatatta atggtaaacg ggatcatagg ttgttgcacg aaagaatcat ggagattaca
     1201 ccgcagcaac tggttgaatt cactatacct gatgggcggg ggcagcaagc cagaacactg
     1261 cctgacatgc tttcatcaga cgcaggtcct ctctcatatc ctctgattta ctccagtaag
     1321 gtgatgatca tatctttatt tgatagtacg gtaatgccag ataaatacag acacaatggt
     1381 aaataatata gttgcaaata atgctgccat aatcagcgcg attgcgacac ctgttttcga
     1441 taccagcggc accagcaaag cgcacagtcc atagccaagt gtgtgactgg tagcgagcca
     1501 gcccgcgcct gcgccatcag ccagcttatc attcagtaga agttgataag cgggtgttgc
     1561 cagagctgct ccaaatgaca gcactgcaca gcccgggtaa aacaaccata gctgttcgga
     1621 gagcatgata gccagaccac cactcatcaa cactccggca ctcaggagca gggccaccgg
     1681 agtcaggcgc tgcggacgga gtaccccgaa ctgcgcgata agcgcagcta ctgcggacag
     1741 acccaacagc caggccacct gttggctaat ggcggtggta tccgtgacga actggcgagt
     1801 aagggcaggc gaaagtccaa gctgcatcat gctcaccgcc gcagccagta atattgcgca
     1861 aagcagataa ggcagacaat cccgcttcag actgacgctc ttgcactccg gtgtgggctg
     1921 tggtggtgtg ccgggcaacc gcagcagcat cagcagcgcc agcactggtg ctgccatcag
     1981 tagccccagt ggcgccagag gatgaatggc cagcattgct gccgcgcaca gcggcccgaa
     2041 tagcctgccg caactcaggc cggagctgat ggttgccaga gcggccatgc gattcccttc
     2101 tccagctctc tgcaatgccc agacctgaca ggctggcacc attgccgaca cggtcagccc
     2161 gtaggcgatc cgggcgatga ttaatattcc cagtcccacc gctgttgtta tggcgctggt
     2221 agccatcagc acgcttccca gccctagcag ggtaaagctt atgccatatc ctcccagagc
     2281 ccacaacacc acaggtttac tgccagtacg ggaaatttgc tttccccacc acggggacga
     2341 tggcagaaac agcattgagc caagcatcag cagggcagcc catacagaca gactcagatt
     2401 tgtctgtatg accagaacag ggagcacaac cagcagaccg ttctgtccga taccgagaag
     2461 cccggcactg aacgcaagtg gccagcagga cagtgttttt tggggcgtat cttcgatccc
     2521 tggtgacatt ataatgtttc aactccatgt attaattgtg tttatttgta aaattaattt
     2581 atctgacaat aacatttatt gttgataatg agaatcatta tcgacataat tgttattatt
     2641 ttactgtgtg ggagctgttt gactatgacc ctgccctctg aaaaaccagc cacagatgtg
     2701 gctgcgcagt gcttcctgaa tgcactgatt cgtgaaacca cagactggaa actgacagaa
     2761 tacccgccag acgaattgct catcccgctg gatgagcaga aatcgctcca tttcagagtg
     2821 gcttatttct ccccaaccca gcatcaccgc tttgcatttc ctgcacgtct ggtcacggca
     2881 tcaggcagtt atcctgtcga ctttaccact ctctcccggc ttattattga taagctacgc
     2941 catcaactgt ttctgcccgt tcccctctgc gaaactttcc accagcgcgt gctggaaagc
     3001 catgtccata cgcaacaggc aattgatgcc cgtcatgact gggccgctct gcgtgaaaaa
     3061 gcgttgaatt ttggcgaggc tgagcaggcg ctgctgacag gacacgcttt ccaccctgcg
     3121 cctaagtctc atgaaccgtt taaccgacgg gaggctgaac gctacctgcc tgatatggca
     3181 ccccacttcc cactgcggtg gttttcggtg gataaaacgc aaatcgccgg tgaaagtttg
     3241 catctcaacc ttcaacagcg gctgacgcga tttgccgcag agaatgcgcc tcagttactc
     3301 aacgaattaa gtgacaacca atggctgttc ccgttgcacc cgtggcaggg ggaatatctt
     3361 ttgcagcagg ggtggtgcca ggcacttgtt gctaaagggc tgattaaaga cttaggtgag
     3421 gccggcacgt cgtggctgcc gaccacctct tcccgttccc tctactgtgc caccagccgc
     3481 gatatgatca agttctccct gagcgtacgg ctgaccaact ccatccgtac tctgtccgtg
     3541 aaagaagtga agcgtgggat gcgcctggca cgcctggctc aaaccgacgg ctggcagatg
     3601 ctacaggtcc gcttcccgac tttccgggta atgcaggagg atggctgggc cgggctgctc
     3661 gatcttaacg gcaacatcat gcaggaaagt ctgtttgccc tgcgtgaaaa tctgctggtg
     3721 gatcagccga aaagccagac taacgtactg gtctccctga ctcaggccgc acctgatggc
     3781 ggtgattcgc tgctggtttc ggcggtaaaa cgcctgagcg atcgcctcgg tatcactgtg
     3841 caacaggccg cccatgcatg ggtcgatgca tactgtcagc aagtgctaaa gccgctgttt
     3901 acggctgaag cggattacgg cctggtgctg ctggcgcatc agcaaaatat tcttgtccag
     3961 atgcttgggg atctgccggt cggatttatt taccgtgact gtcagggcag cgcttttatg
     4021 cctcacgcga cagactggct cgattctatt ggcgaggcgc aggcggaaaa tattttcacc
     4081 catgagcagt tgctgcgcta tttcccttat tacctgctgg ttaactccac ttttgctgtg
     4141 accgctgcgc tgggggctgc cgggctggac agcgaatcga atctgatggc tcgtgtacga
     4201 gcatcgctgg ctgaagtgcg tgatcaggtg actcataaaa cctgtctcaa ctacgtgctg
     4261 gaaagtccgt actggaacgt aaaaggtaac tttttctgtt atctgaacga tcataacgag
     4321 aacaccattg ttgacccttc ggtgatctat ttcgatttcg ctaacccgct gcaggctcag
     4381 gaggtctgaa tgtctgaggc aaacattatt cacagcagat atggactgcg ctgtgaaaaa
     4441 ctcgacaagc ccctgaatct tggctgggga ctggacaata gcgcggtgtt gcactgtccc
     4501 ggggagctgc cgacagggtg gctgtgcgat gcgctggatc agatatttat cgccgcacca
     4561 caactgtcag cagtggctct cccctgggcc gaatggcgtg aggagccaca ggcgctgacg
     4621 ctttttgggc aggtaaaaag cgacattatc catcgtactg ccttctggca gttaccgtta
     4681 tggttgagtt caccggcaaa ccgggcctcc ggtgaaatgg tttttgatgc agagcgtgag
     4741 atttatttcc cgcagcgccc accccgtcca cagggtgagg tttaccgccg ttacgatcca
     4801 cgtattcgca ggatgctgag tttccgcatt gccgatcccg tttctgatgc agaacgtttc
     4861 acccgctgga tgaatgatcc gcgcgttgag tatttctggg agcaaagtgg ctcactggag
     4921 gtacagaccg cctatctgga gcgccagctc accggtaaac atgcattccc gctgattggg
     4981 tgcttcgacg atcggccatt cagctatttc gaaatctact gggcggcgga agaccgcatt
     5041 ggtcgtcact attcgtggca gccctttgac cgtggcctgc atctgctggt tggtgaacag
     5101 caatggcgcg gggcccacta tgtgcaaagc tggctgcgcg ggctgacaca ttacctgctg
     5161 ctggatgagc cccgtacgca gcgcacagta ctggagccac gcaccgataa ccagcgcctg
     5221 ttccgccatc ttgagcctgc gggataccgg acaattaaag agttcgactt cccacataag
     5281 cgctcgcgca tggtgatggc ggatcgtcat cacttcttca cggaggtcgg tctgtaatga
     5341 atcacaagga ctgggatttt gttaaccgtc ggctggtggc aaaaatgttg tctgagatgg
     5401 agtatgagca ggttttccac gcggaatctc agggcgatga ccactactgc attaacctgc
     5461 cgggagcaca atggcgcttc atcgctgaac gtggtatctg gggctggctg tggattgatg
     5521 cccaaacgtt gcgctgcacg gacgaaccag tactggcgca gacgctgctg atgcagctaa
     5581 agccggtgct gtcaatgagt gatgccactg ttgcagagca tatgcaggat ttgtatgcca
     5641 cgctactggg cgacctgcaa ctgctgaagg cccgtcgcgg actgagtgcc agtgacctga
     5701 ttaatcttga tgccgatcgt ctgcaatgcc tgctgagcgg tcatcctaaa ttcgttttta
     5761 ataaaggtcg ccgtggctgg ggcaaagagg cgctggaacg atatgcgcca gagtatacca
     5821 acaccttcag actgcactgg ctggcggtaa aacgtgaaca tatgatctgg cgctgcgata
     5881 acgatctgga tattcagcag ttgttgactg ccgcaatgga tccgcaggag tttacacgct
     5941 tcagtcaggt ctggcaggaa aacgggctgg atcacaactg gctaccgctg ccggtacatc
     6001 cgtggcagtg gcagcaaaaa atcgccacag acttcatcgc tgattttgtc gaaggcagga
     6061 tggtgtctct cggcgagttt ggcgaccagt ggctcgccca gcagtcgctg cgtaccctga
     6121 ccaacgccag ccgccgggga gggctggata tcaagctgcc gctgaccatc tacaacacct
     6181 catgctaccg gggaatccct ggcagatata tcgctgccgg accactggct tcacgctggc
     6241 tgcaacaggt ttttgcgacc gacgccaccc tggtgcaaag cggcgcagtg atccttggtg
     6301 aaccggctgc aggctatgtg tcccatgaag gctatgccgc gcttgcccgg gctccctatc
     6361 gctaccagga aatgcttggt gttatctggc gggagaatcc gtgccgctgg ctgaaaccgg
     6421 atgaaagtcc tgttctgatg gcaacactga tggagtgcga cgaaaacaat cagccgctgg
     6481 caggcgcata tatcgaccgc tccggactgg acgctgaaac ctggcttacg caattgttcc
     6541 gggtggttgt ggttccgctg tatcacctgc tttgccgcta cggtgtcgcg cttattgcac
     6601 acggacaaaa tataacgctc gccatgaaag agggggtacc acagcgtgtt ctgctgaaag
     6661 acttccaggg cgatatgcgg ctggtgaaag aagcgtttcc cgaaatggac tctttgcctc
     6721 aggaggtgcg tgatgccaca tcccgtctga gtgcggacta cttaatccat gatttgcaga
     6781 cgggtcactt cgtgacagta ctgcgtttta tttcgccact gatggttcgt cttggcgtac
     6841 ctgaaaggcg attttatcaa ctgctggcag cagtgttgag tgattacatg aacaaacatc
     6901 cacaaatggc agagcgtttt gcgcttttct cactcttcag gccacaaatc attcgcgtgg
     6961 tgctgaaccc ggtaaaactg acctggccgg acctggatgg cggcagtcgc atgctgccga
     7021 attaccttga gaatctgcaa aatccgctgt ggctggtaac tcaggaatat gaatcatgaa
     7081 aaaaagtgtc gattttattg gtgtcgggac agggccattt aatctcagca ttgctgcatt
     7141 gtcacatcag atcgaagaac tgaactgtct cttctttgac gaacatcctc atttttcctg
     7201 gcatccgggt atgctggtac cggattgtca tatgcagacc gtctttctga aagatctggt
     7261 cagtgcagtt gcacctacaa atccctacag ctttgttaac tatctggtga agcacaaaaa
     7321 gttctatcgc ttccttacaa gcaggctacg tacagtatcc cgtgaagagt tttctgacta
     7381 tctccgctgg gctgctgaag atatgaataa cctgtatttc agtcataccg ttgaaaatat
     7441 tgatttcgac aaaaaaagtc gattgtttct ggttcagacc agtcggggag aatattttgc
     7501 ccgcaatatc tgccttggta caggaaaaca accttattta ccaccctgtg tgaagcatgt
     7561 gacacaatcc tgtttccatg ccagtgaaat gaatcttcgt cggccggacc tgagtggaaa
     7621 acggataacc gtggttggtg gaggacagag tggtgcagac ctgttcctta atgcattacg
     7681 cggggaatgg ggagaagcgg cggaaataaa ctgggtctca cggcgtaata attttaacgc
     7741 actggatgag gctgcttttg ctgatgagta ttttacacct gaatatattt caggcttctc
     7801 cggactgaag gaagatattc gccatcagtt actggatgag cagaaaatga catcggatgg
     7861 catcactgcc gattctttac tgaccattta tcgtgagttg taccaccgtt ttgaagttct
     7921 gagaaaacca agaaatatcc gtctgctacc cagtcgctcg gtaacaaccc tggaaagtag
     7981 tggtccgggc tggaggttat tgatggaaca tcatctggat cagggcagag agagcctgga
     8041 aagtgatgtg gtgattttcg ccacaggtta ccgttctgca ttgccacaaa tacttccctc
     8101 actgatgccc ttgatcacca tgcacgataa gaacaccttt aaagtgcgtg atgacttcac
     8161 tctggaatgg agtggcccga aagaaaataa catctttgcg gtcaacgcca gcatgcaaac
     8221 tcatggcatc gccgaacccc agctcagcct gatggcctgg cgatctgcac gtattcttaa
     8281 tcgcgtactg gggcgtgatt tattcgatct cagtatgccg cccgcactga ttcagtggcg
     8341 cagcggcagc cggaaaaaac cgcagccgga ggctgcttct ttaactcact atacagcaaa
     8401 tattcaggaa taacgatgat gcgcaaaaag tatatgcccc gggctcttgg tccgctgctt
     8461 cttgtcgtgc tgtcaccagc tgtcgcccag caaaacgatg ataatgagat catagtgtct
     8521 gccagccgca gcaatcgaac tgtagcggag atggcgcaaa ccacctgggt tatcgaaaat
     8581 gccgaactgg agcagcagat tcagggcggt aaagagctga aagacgcact ggctcagtta
     8641 atccccggcc ttgatgtcag cagccagagt cgaaccaact acggtatgaa catgcgtggc
     8701 cgcccgctgg ttgtcctgat tgacggtgtg cgcctcaact cttcacgttc cgacagccga
     8761 caactggact ctgtcgatcc ttttaatatc gaccatattg aagtgatctc cggcgcgacg
     8821 gccctgtacg gtggcgggag taccggaggg ttgatcaaca tcgtgaccaa aaaaggtcag
     8881 ccggaaacca tgatggagtt tgaggctggc acaaaaagtg gctttaacag cagtaaagat
     8941 cacgatgagc gcattgccgg tgctgtctcc ggcggaaatg accatatctc cggacgtctt
     9001 tccgtggcat atcagaaatt tggcggctgg tttgacggta acggcgatgc caccctgctt
     9061 gataacaccc agaccggcct gcagcactcc aatcggctgg acatcatggg aaccggtacg
     9121 ctgaacatcg atgaatcccg gcagcttcaa ctgataacgc agtactataa aagtcaggga
     9181 gacgacaatt acgggcttaa tctcgggaaa ggcttttccg ccatcagcgg gagcagcaca
     9241 ccatacgtca gtaaggggct gaattctgac cgcattcccg gcactgagcg gcatttgatc
     9301 agcctgcagt actctgacag tgatttcctg ggacaggaac tggtcggtca ggtttactac
     9361 cgcgatgagt cgttgcggta ctacccgttc ccgacggtaa atgcgaataa acaggcgaca
     9421 gctttctcct cgtcacagca ggataccgac cagtacggca tgaaactgac tctgaacagc
     9481 caactgatgg acggctggca aatcacctgg gggctggatg ctgagcatga gcgctttacc
     9541 tccaaccaga tgttcttcga tctggctcag gcaagtgctt ccggagggct gaacaaccat
     9601 aagatttaca ccaccgggcg ctatccgtca tatgacatca ccaatctggc ggccttcctg
     9661 caatccagct atgacattaa tgatattttt accgttagcg gtggcgtacg ctatcagtat
     9721 actgagaaca gggtagatga tttcatcgac tacacgcagc aacagaagat tgctgccggg
     9781 aaggcgatat ctgccgacgc cattcctggt ggttcggtag attacgataa ctttctgttc
     9841 aatgctggtc tgctgatgca catcaccgaa cgtcagcagg catggttcaa tttttcccag
     9901 ggggtggcat tgccggatcc ggggaaatat tatggtcgcg gcatctatgg tgcagcagtg
     9961 aacggccatc tttccctgac aaagagcgtg aacgtcagcg acagtaagct ggaaggcgtg
    10021 aaagtcgatt cttatgaact gggctggcgc tttaccggtg acaacctgcg gactcaaatc
    10081 gcggcatatt actcgctttc caataagagc gtggaaagga ataaagatct gaccatcagt
    10141 gtgaaggacg acaggcgccg tatttacggc gtggaaggtg cggtggacta cctgatcccg
    10201 gatactgact ggagtaccgg tgtgaacttc aatgtgctga aaaccgagtc gaaagtgaac
    10261 ggtcaatggc aaaaatatga cgtgaaggaa tcaagtccat cgaaagcgac agcttacatt
    10321 aactgggcgc cggaaccgtg gagtctgcgt gtacagagca ccacttcttt cgacgtaagc
    10381 gatgcagagg gtaacgatat taatggttac actaccgtcg attttatcag tagttggcag
    10441 cttccggtgg gaacactcag cttcagcgtt gagaacctct tcgaccgtga ctataccact
    10501 gtctggggac agcgtgcacc tctgtactac tgcccgggtt acggccctgc ttcactgtac
    10561 gactacaaag gccggggccg aacctttggt ctgaactact cagtgctgtt ctgacgggga
    10621 tgcttttgca acaagggtat gctgtaaaca acatggtcct tggtggccat gttgtttaca
    10681 gccagtataa tatacagcct taacaagata ttccgtctcc ggaatggact gttcaccact
    10741 gaataacatt atttctgttt tatggatctt tgactgaaat ttatcgcata tcctcatttc
    10801 attgtccgtc tgaaaactaa ccttacaagt aagcgtcgtc tcagcaccgt ctggcagatc
    10861 ctgaaattcc tgagaggatg atctgcgccc aatatcctga gccggaactt tgctgcaatg
    10921 aaggccaacg aaaaatgggg gagtatgtta tagagtactt gctacagggt aaaaagaggt
    10981 acctgtcgct ggttctccgt tcagtttaac cgggagattt tctcctgcgc ctgtcataaa
    11041 gtccggtaat ggagattgtt aatacgatgg tgcacgatgc attgtcggtg cccggcccgg
    11101 aggatgcccc gtaactacat acggatcagg gttggcaata tcgaatggca ggctataggc
    11161 aaagttaaag ccccatggag tagcacaaaa tatgccacac aaaggaaacg gtctgaataa
    11221 cgcagtgatg gagaacttct tcggcacact gcaaaatcgc atcgtggtcg atcgctaatt
    11281 cgccgtgaat cactgaacga tgttccatgc gaacagatat tcgatcatat cgaaatatta
    11341 aacattaccc ctccccgcat tgggcaaaaa aaaaacaaaa aacgatcaat tagtattgat
    11401 tcacataatc tctcatcatt aatgagaaca ttgtcacata tttacaatat agaactgtta
    11461 cttttagtaa gctgttagta accaactgtt actaataatt ttaattctta atactttgtt
    11521 cattcaataa atgagttgag ag
//
LOCUS       DADMKF010000127        11428 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.129, whole genome shotgun sequence.
ACCESSION   DADMKF010000127 DADMKF010000000
VERSION     DADMKF010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11428
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.129"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(42..383)
                     /gene="bamE"
                     /locus_tag="JNC24_003109"
     CDS             complement(42..383)
                     /gene="bamE"
                     /locus_tag="JNC24_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HAY9159797.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(532..2193)
                     /gene="recN"
                     /locus_tag="JNC24_003110"
     CDS             complement(532..2193)
                     /gene="recN"
                     /locus_tag="JNC24_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HAY9159798.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALEIARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKFDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLNKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            complement(2279..3157)
                     /gene="nadK"
                     /locus_tag="JNC24_003111"
     CDS             complement(2279..3157)
                     /gene="nadK"
                     /locus_tag="JNC24_003111"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HAY9159799.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            3280..3873
                     /gene="grpE"
                     /locus_tag="JNC24_003112"
     CDS             3280..3873
                     /gene="grpE"
                     /locus_tag="JNC24_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HAY9159800.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            complement(3928..5214)
                     /locus_tag="JNC24_003113"
     CDS             complement(3928..5214)
                     /locus_tag="JNC24_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAY9159801.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGEYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(5235..6101)
                     /locus_tag="JNC24_003114"
     CDS             complement(5235..6101)
                     /locus_tag="JNC24_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311501.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HAY9159802.1"
                     /translation="MQRLEQRSPDAILLLFLIAQTVDITMPVFALLALVAYSVSLALI
                     VPGLLQKNGGWRRMAIISAVIALVCHAIALEARILPDGDSGQNLSLLNVGSLVSLMIC
                     TVMTIVASRNRGWLLLPIVYAFALINLALATFMPNEYITHLEATPGMLVHIGLSLFSY
                     ATLIIAALYALQLAWIDYQLKNKKLAFNQEMPPLMSIERKMFHITQIGVVLLTLTLCT
                     GLFYMHNLFSMENIDKAVLSIVAWFVYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLA
                     YFGSRIVQQLIS"
     gene            6193..7554
                     /gene="ffh"
                     /locus_tag="JNC24_003115"
     CDS             6193..7554
                     /gene="ffh"
                     /locus_tag="JNC24_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HAY9159803.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGKFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            7803..8051
                     /gene="rpsP"
                     /locus_tag="JNC24_003116"
     CDS             7803..8051
                     /gene="rpsP"
                     /locus_tag="JNC24_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HAY9159804.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            8070..8618
                     /gene="rimM"
                     /locus_tag="JNC24_003117"
     CDS             8070..8618
                     /gene="rimM"
                     /locus_tag="JNC24_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HAY9159805.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            8649..9416
                     /gene="trmD"
                     /locus_tag="JNC24_003118"
     CDS             8649..9416
                     /gene="trmD"
                     /locus_tag="JNC24_003118"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HAY9159806.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            9458..9805
                     /gene="rplS"
                     /locus_tag="JNC24_003119"
     CDS             9458..9805
                     /gene="rplS"
                     /locus_tag="JNC24_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HAY9159807.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(9882..10364)
                     /locus_tag="JNC24_003120"
     CDS             complement(9882..10364)
                     /locus_tag="JNC24_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HAY9159808.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAIGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(10380..>11428)
                     /gene="dgcN"
                     /locus_tag="JNC24_003121"
     CDS             complement(10380..>11428)
                     /gene="dgcN"
                     /locus_tag="JNC24_003121"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
                     /protein_id="HAY9159809.1"
                     /translation="TYSLEAAVVFADGPAATETLAALGQQGQFSTAEVRDKQQNILAS
                     WHYTRKDPGDTFSNFISHWLFPAPIIQPIRHNGETIGEVRLTARDSSISHFIWFSLAV
                     LTGCILLASGIAITLTRHLHNGLVEALKNITDVVHDVRSNRNFSRRVSEERIAEFHRF
                     ALDFNSLLDEMEEWQLRLQAKNAQLLRTALHDPLTGLANRAAFRSGINTLMNNSDARK
                     TSALLFLDGDNFKYINDTWGHATGDRVLIEIAKRLAEVGGLRHKAYRLGGDEFAMVLY
                     DVQSESEVQQICSALTQIFNLPFDLHNGHQTTMTLSIGYAMTIEHASAEKLQELADHN
                     MYQAKHQRAEKLVR"
BASE COUNT         2803 a         2747 c         3061 g         2817 t
ORIGIN      
        1 attgagcacc ttttttaacg tctttgagag caactttatt attagttacc actcagcgca
       61 ggtttgttat caatattggt caacacaccg ctactgttaa aggtcagcgt cagcgtttgc
      121 tgcgttacac cttcatgacc tggttgctgg cggaagacat agaaccaggt attcgtacca
      181 aatggatcgg acatcagcgg tgtacccaat gcgtacgcaa cttgttgttg cgtcatgcca
      241 acacgtattt tggatacgtc gttagcggtc agatagttcc cctggttgat gtcaggacgg
      301 taaaccactc gctccagagt ggaacagcct gcggtcaaca tcaatagtac tgctgctgca
      361 gcagtcagcg ttttacagcg catagtgatt tgattccttt tcgggcccga gcagtacgtg
      421 gctcatctgt aatatgccga tgataataga cctttcatca ctttaaaacc ttttgctttc
      481 cggtcttacg gcgttttgct gtttactcta accgtgaagc aggaaaaaag tttacgctgc
      541 aagcagttct ttcgcattcg ctagtgtatt acgtgtgact tcactgccac caagcaggcg
      601 cgccagctct tgtaaccgcg cttttttatt cagggattgc atatgcgttt ctgtcatcgc
      661 accatcggtt tctttgctga caaaatagtg ttgatgacca catcccgcga cttgtggcag
      721 gtgggtaaca cacatcacct gagttgattc gccgagttga cgcagcagtt tgccgacaac
      781 tgccgctgtt ggaccgctaa tccctacatc cacttcatca aaaatcagtg ccggggtttc
      841 cattttacgc gccgtgatga cctggattgc cagtgcgatg cgggacaatt caccaccgga
      901 tgcgactttg gcaataggct gcattggctg acctgggttg gtggttaccc gaaactcaat
      961 acgatcggca ccgtcagcgc ccaggtgatg ctcgtcaaat ttaacatcga tcgtaaactg
     1021 cccatgcggc attgagagcg catgcatact gtcggtgatc agctgtgcaa gttcttctgc
     1081 atattgctgg cgttgttggt gtaatgcgcg cgcgatttcc agtgcctgct gatgatgttt
     1141 cgttaccgcc agcgcaagcg tttcttgtga gtcggcctga tcgtccagtt gctgctgttc
     1201 ttccagtagc gactggtaat actgtggcaa tgcctcaggg ctgacgtgat gtttacgtgc
     1261 cagcgaaatc tgttttgaga tgcgctgttc aagttcaaat agtcggttgg gatcgagatc
     1321 cagacgatcg cagtagtggc gcagttcatc gctggcttca gcaatctgga tggtagcttc
     1381 ttccagcata tcaagtacgc cggacagttt gctgtccatg ccaatcaatt cgctcaccag
     1441 ttgtttagcc gtgtaaagct gactttgcag gtttgcgtct tcaccgtcgg ccattaatgc
     1501 caatgcattc tggctggtgg tcagcaattg accgctgttc gccagacgtt tgtactcttc
     1561 gtcgatttgt tcaaactctc cgggctgcgg attaaattcg ttaagttctt ttaattggta
     1621 ttgcagcagt tccgcacggg cggcgcgttc ctgacttaac tgttgatgat gcgcgaggtc
     1681 acggcagctt tgatgccaca actgataacg tgcggtcatt tcctgcagta gagaggtttc
     1741 attggcatag ccatcaagca ggaatttttg gtgctcaggt ttggtgagta attgatgagc
     1801 gtgctgacca tggatctgaa tcagcaactg acccagttcg cgcagttgtg acagaggaac
     1861 agctgtaccg ttgatgaaac cacgggagcg accatcgctg ctgatcacgc gacgaagcaa
     1921 acattcatgc ccgtcttcaa gctggttttc ttccagccag cgcagagccg ctggcgtatc
     1981 tttcagagaa aaacgggcgc acaggtcagc gcgagcagcg ccggtacgca ccatgtcggc
     2041 ttcagcgcga ccaccgagac aaagaccgag ggcatctatt gcaatagatt tacccgcgcc
     2101 ggtctcgcca gttattacgg tcatgccgct atgaaaatca atctcaagct cacgaacgat
     2161 agcaaagttg ctgatggtca gttgtgccaa catagtcgct ttcctgtatg aaaaaccatt
     2221 actgttattg tgtacagtat aaactggttt tatatacagt aaagaggctg gcgtaaaatt
     2281 agaataattt ttttgaccag ccgagcttgg tgcttaatgt gttgaaataa ctgtaatctt
     2341 tcggatgaat cagattcaga tggtaatcac agcgacgaat caggacatct tcaccttcct
     2401 gaatcggcag tgctatctgg ctgtcgcaac tgatttccag gtcgttacgg cgatgcgaaa
     2461 aacgcagacg gatcgtgctg ctgctgttta tgaccagtgg tcgtgctgac aacgtatgcg
     2521 ggaacatggg caccagggta atcgcatcca gagagggggt cagaatagga ccgcctgcag
     2581 agagggaata ggcggtggag cctgttggcg tcgaaataat tagtccatca gatcgctgag
     2641 aaaacgcaaa gatctcgtcg atatacactt cgaactcaat catatgcgcc actttgcctg
     2701 gatgaagtac cacttcattt atcgcggtgc tgatgcgttt ctggcaatct tgctgacaga
     2761 cttgcgcttc cagcaaaaaa cgtttctcgc tgatgtagtg gccttccagc acatcggcta
     2821 actgttgctg ggcgttatcg gggtcaaggt cagtcaggaa acccaggttg ccacggttga
     2881 ttccaataac tttaatatcg taacgggcga gtgtgcgtgc cgcgcccagc atattaccgt
     2941 cgccaccaac gactaccgcg agatcagcta gttgcccaat ctccgcgagc gtgccagttt
     3001 tcacattctt cagttgcagt tcgtgagcga tttgttgctc aacgatgacc tcgtaacctt
     3061 ttgtgcacag ccagcggtag agcatttcat gtgttgtcag tgcagtgggg tgccgtgggt
     3121 gtcccacaat gccaatacac ttgaaatgat tattcatttt tccgaggtcc ttgttgcgaa
     3181 gattgatgac aatgtgagtg cttcccttga aaccctgaaa ctgatcccca taataagcga
     3241 agttagcgag atgaatgcga aaaaaacgcg gagaaattca tgagtagtaa agaacagaaa
     3301 acgcctgagg ggcaagcccc ggaagaaatt atcatggatc agcacgaaga gattgaggca
     3361 gttgagccag aagcttctgc tgagcaggtg gatccgcgcg atgaaaaaat tgcgaatctc
     3421 gaagctcagc tggctgaagc ccagacccgt gaacgtgacg gcattttgcg tgtaaaagcc
     3481 gaaatggaaa acctgcgtcg acgtactgaa ctggatattg aaaaagccca caaattcgcg
     3541 ctggagaaat tcatcaacga attgctgccg gtgattgata gcctggatcg tgcgctggaa
     3601 gtggctgata aagctaaccc ggatatgtct gcgatggttg aaggcattga gctgacgctg
     3661 aagtcgatgc tggatgttgt gcgtaagttt ggtgttgaag tgatcgccga aactaacgtg
     3721 ccgctggacc cgaatgtgca tcaggctatc gcaatggtgg aatctgatga cgttgcgcca
     3781 ggtaacgtac tgggcattat gcagaagggt tatacgctga atggtcgtac gattcgtgcg
     3841 gcgatggtaa ctgtagcgaa agcaaaagcg taatttctgt tttcgtaata attcacggcc
     3901 ctgcatgcga atgcagggcc gttttcgtta ctccgccaca ctctcgcgca gcggtttcac
     3961 aggaaaaact tttacctgct taatcatatt gtcctgtacg tcgagaatat ctatatcgta
     4021 ctcgccaata cgcacgcggg tgcctgcgac agggatctct tccagtgcct caagaatgac
     4081 gccattaacc gtgcgggcat catcttccgg tagatgccag ttaaaggctt tgttgatttc
     4141 ccgcacgttg gcggtgccat cgataatcac cgaaccgtcg ttttgcggcg tgacctcttc
     4201 ggcaagtgtt ggcgacatcg acgtagtgaa atcgccgaca atctcttcca gaatatcttc
     4261 aaccgtcacc agcccctgaa tgtctccata ctcgttgacg accaggccga ctttcttttt
     4321 gttgcgctga aactttacca actgcgtgct gagcggcgta ccttccggca caaaatagat
     4381 ctcgtccgcg gcgcgcagca tggtttcttt ggtgaactct tttttctccg acatcaaccg
     4441 ccaggcttca cgtactcgca gcatactgat ggcgtcgtcc agcgaatcac ggtagagcac
     4501 gatgcgcccg tgaggtgagt gggagagttg gcgcagaatc gatttccagt catcgttgat
     4561 atcaataccg ataatttcac tgcgcggcac catgatgtca tcaacggtca ttttttccag
     4621 atcgagcacc gacagcagca tatcctgatt gcgacgggaa atttgtgagc gtgattcgtg
     4681 cacgatagtg cgcaactctt ctttgctcaa agagccgcta accacgatat cggttttgat
     4741 acccatcatg cgcatcagca tacgggtgat agcattcagc aaccagacca gcggcatcat
     4801 caaaatttgc agcggagcca gcagaaaact actcggataa gcgacttttt ccgggtacag
     4861 cgcggcaatg gttttcggca atacctcagc aaataccagt acgacaaaag tcagcacacc
     4921 agtcgcaatt gccacgcccg catcgccgta caaacgcatc ccaacaatag tgccgagcgc
     4981 ggaggcaaga atattgacca ggttattgcc gattaacacc aggcttatca ggcggtctgg
     5041 cttacgcagc aatttttcga cgcgtttggc cgagcgatta ccctgtttcg ccatatgtcg
     5101 cagacgatag cggttgaggg tcatcattcc ggtttcggac ccggaaaaat aggctgaaat
     5161 gaccaccatg atgatcagaa taatgatcaa cgtagtagta gaaatgtgtt ccaggggaaa
     5221 ctccttttct gggtttagct gattaactgc tggacaattc ggctgccgaa gtaggccagt
     5281 gtcagaatta ccgcgcccgc aacgttaaac cagacgacgc ggcgtccacg ccatccttca
     5341 tggtaatgtc cccacagcag cacaatatag acaaaccacg ccacgataga gagcacagcc
     5401 ttgtcgatat tttccatgct aaataggttg tgcatgtaga acaggccagt gcaaagcgtg
     5461 agcgttagca gcaccacgcc aatctgcgtg atgtggaaca ttttacgctc gatactcatc
     5521 aatggcggca tttcctggtt aaatgccagc ttcttgttct tcagttggta atcaatccac
     5581 gccagttgca gcgcgtacag ggcggcgata attagcgtgg cataggaaaa gagcgataag
     5641 ccaatgtgca ccagcatccc aggcgttgct tccagatggg tgatgtattc attgggcatg
     5701 aaggtcgcca gcgccaggtt gataagcgca aaggcataga caatgggtag cagcaaccag
     5761 ccacgattgc gagaagccac aatggtcatt accgtacaga tcatcaaact gaccaatgaa
     5821 ccaacgttca gcaggctgag gttttgtccg ctatcaccgt cgggcaggat gcgggcttcc
     5881 agagcgattg cgtggcagac cagcgcaatg accgcagaaa taatagccat gcgccgccag
     5941 ccgccgtttt tttgcagcag accgggaaca atcagcgcaa gactgacgga gtaggcgaca
     6001 agcgcgagca gagcaaaaac gggcatagtg atgtcgacag tttgagcaat aagaaagaga
     6061 agcagtatag cgtcaggtga acgctgctcc aaccgttgca taacaacaaa gacgccttca
     6121 tgttatactg cggcaaaata ctgatgatgt gtcgcgattg cggccaaccg tttccacccc
     6181 aggcgagaga caatgtttga taatttaacc gatcgtttgt cgcgcacgct gcgcaatatc
     6241 agtggccgtg gacgcctcac tgaagacaac gtaaaagata cgctgcgcga agtgcgcatg
     6301 gcgctgctgg aggcggatgt cgctctgccg gtagtgcgtg agtttatcaa tcgcgtaaaa
     6361 gagaaagcgg ttggtcatga agttaataag agcctgacgc cggggcagga gttcgtcaaa
     6421 atcgtccgta acgaactggt tgcggcgatg ggcgaagaga accagaccct gaacctggcg
     6481 gcgcaaccgc ctgcggtcgt gctgatggcg ggcctgcaag gtgccggtaa aacaaccagc
     6541 gttggtaaac tcggtaagtt cctgcgcgag aagcacaaga agaaagtgct ggtggtttct
     6601 gccgacgttt atcgcccggc ggcaatcaaa cagcttgaga cgctggcaga gcaggtgggc
     6661 gttgatttct tcccttctga tgttggtcag aagccggtag atatcgttaa cgcggcgctg
     6721 aaagaagcca aactgaaatt ctacgacgtg ctgctggtgg ataccgctgg tcgtctgcac
     6781 gttgacgaag cgatgatgga cgagatcaaa caagtccatg cgtcgattaa cccggttgaa
     6841 accctgtttg tggttgacgc catgaccggt caggatgcgg ccaatacggc aaaagcattc
     6901 aatgaagcgt taccgcttac cggcgtagtg ttgaccaaag tggacggcga tgcccgcggc
     6961 ggtgcggcgc tctctattcg tcacatcact ggcaaaccga tcaagtttct cggtgttggc
     7021 gagaaaactg aggcgctgga gccgttccat ccggaccgca tcgcgtcgcg tattctcggc
     7081 atgggcgacg tactgtcgct gatcgaagat atcgaaagca aagttgaccg cgcgcaggca
     7141 gagaaattag ccagcaagct gaaaaaaggc gacggcttcg atctcaacga ttttcttgag
     7201 cagctgcgcc agatgaaaaa tatgggcggc atggctagcc tgatgggcaa gctgccgggc
     7261 atggggcaga tcccggataa tgttaagtcg cagatggacg ataaagtgct ggtgcgtatg
     7321 gaagccatca tcaactcgat gacgatgaaa gagcgcgcta agccagaaat catcaaaggt
     7381 tcgcgtaagc gtcgtatcgc tgccggttgc ggtatgcagg tgcaggacgt taaccgcctt
     7441 ctgaagcagt tcgacgacat gcagcgcatg atgaagaaaa tgaagaaggg cggaatggcg
     7501 aagatgatga gaagcatgaa gggtatgatg cccccaggct tccctggtcg ctaatcggcc
     7561 tggcttgttt gccattttgg caccgggatg tgctcgtcat cctcacgtac tgcgtgtacg
     7621 ctccggtgac tgcgcgcact ccggcaccaa actggctgca ccgccgacgg ccttttacag
     7681 cagagttagc aactgttgat tgcaattccc gcaaaaatga gtaaaatttt cgggctttta
     7741 atatgacacc ggactccgtt cctcgatggg gtccggttgt tttattcaca caagaggatg
     7801 ttatggtaac tattcgttta gcacgtcacg gcgctaaaaa gcgtccgttc taccaggttg
     7861 ttgtcgctga cagccgtaat gcacgcaacg gtcgcttcat cgagcgcgtt ggtttcttca
     7921 acccaatcgc tagcgaaaaa gaagaaggca ctcgcctgga tctggatcgc atcgctcact
     7981 gggttggcca gggcgcaact atttctgatc gcgttgctgc gctgatcaaa gaagtaaaca
     8041 aagcagctta atctgtcacg gtggtcatga tgagcaaaca actcaccgcg caagcacctg
     8101 ttgatcccat cgttttggga aaaatgggtt cgtcttacgg tattcgtggg tggctcagag
     8161 tgttttcttc caccgaagac gccgaaagca tttttgacta tcagccctgg tttatccaga
     8221 aggcgggtca gtggcagcaa gtccagctgg aaagctggaa gcaccacaat caggacatga
     8281 tcatcaagct gaaaggcgtt gacgatcgtg atgcggcgaa cctgctgacg aattgtgaaa
     8341 ttgtcgtgga ttcatcgcag ctgcctcagc ttgaagaggg ggactactac tggaaagacc
     8401 tgatgggctg ccaggtagta accactgaag gctacgatct cggtaaagtc gtcgatatga
     8461 tggaaaccgg atctaatgac gttctcgtca ttaaggcaaa cctgaaagat gcgtttggta
     8521 tcaaggaacg tctcgtaccg ttcctcgatg ggcaggttat caagaaagtc gatctcacta
     8581 ctcgttcaat cgaagtagat tgggatcctg gtttttaaac caccggataa acggtaaaag
     8641 acggcgctat gtggattggc ataattagcc tgtttcctga aatgttccgc gcaattaccg
     8701 attacggggt aactggccgg gcagttaaaa atggcctgct gagcatccag agttggagtc
     8761 ctcgcgactt cacgcatgac cggcaccgta ccgtggacga tcgtccttac ggtggcggac
     8821 cggggatgtt aatgatggtg caacccttgc gggacgccat tcatgcagca aaagccgcgg
     8881 cgggtgaagg cgcaaaggtg atttatctgt caccacaggg acgcaagctt gatcaagcgg
     8941 gcgtcagcga actggcaacg aatcaaaaat tgattctggt gtgcggtcgc tacgaaggta
     9001 tagatgagcg cgtgatccaa accgaaattg acgaagaatg gtcaatcggc gattacgttc
     9061 tcagtggtgg tgagttacca gcaatgacgc tgattgactc cgtttcccgg tttattccgg
     9121 gagtactggg acatgaagcc tcggcaacgg aagattcctt tgctgaagga ttgctggatt
     9181 gcccgcacta tacgcggcct gaggtgttag aagggatgga agttccgcca gtgttactgt
     9241 cgggcaacca tgccgagata cgtcgctggc gtttgaaaca gtcgctgggc cgtacctggc
     9301 ttagaagacc tgaacttctg gaaaacctgg ctctgactga agagcaagca aggttgctgg
     9361 cggagttcaa aacggaacac gcacaacagc aacataaaca tgatgggatg gcgtaagccc
     9421 ccgagatatc agtttaccca ggataagaga ttaaattatg agcaatatta ttaagcaact
     9481 tgaacaagag cagatgaagc aggacgtacc ttccttccgt ccgggtgata ccgtggaagt
     9541 gaaagtatgg gttgttgaag gttccaaaaa acgtctgcag gcattcgagg gcgtggttat
     9601 cgctattcgt aaccgcggtc tgcactctgc attcactgtt cgtaaaattt ccaacggcga
     9661 aggcgttgag cgtgtcttcc agactcactc tccggtagtt gacagcattt ctgtcaaacg
     9721 tcgtggtgct gttcgtaaag ctaaactgta ctacctgcgt gagcgtactg gtaaggctgc
     9781 tcgtatcaaa gagcgtctta actaagattt cgcttaagcg acatcctgtt aagaagggct
     9841 ggccaattgg ctggcccttt ttttgtctgt ttgctcgtgt tttaaggggt tgtaatcacc
     9901 actgcgacgc ggcggttctc ggcgcggccc tgggcggtct tgttactggc tatgggatat
     9961 ttttttccta aaccctgtgt ggtgagattg ctgcgtggaa tttgtccacc aatagcccat
    10021 gcatcggcca cgacattcgc ccgtttcaat gataagcctt cgttgtaact gtcttcacca
    10081 tagttatcgg tgtgtccatc catacgggca tgtgttagcc ctgtcgaggc caatttggct
    10141 gccatggttt ggatctgttg ctggctttcc gggagcaatt tgtagtcatt ttttgcgaac
    10201 agaatggcat ctgataagcc cagcgaccag tcgccggcgg attcagtaaa tccataagat
    10261 tgcatagcgg cgacttgctc aggagtaaac tttccctgag gggactggca gccagtcagt
    10321 attagtgagg tgaaaatcag gggtgctact aggtgcttta tcatatcgat atatccttgt
    10381 tatctcacca gcttttcggc acgctggtgt ttggcctgat acatattgtg atcggcaagc
    10441 tcttgtaatt tttccgcaga ggcgtgctca atggtcatcg cgtaaccaat gcttaatgtc
    10501 atggtggtct gatggccatt atgaagatca aacgggagat taaagatttg tgtcagtgct
    10561 gagcatatct gctgcacttc agattctgac tgtacatcat agagcaccat agcgaattca
    10621 tcgccgccca ggcggtatgc tttatgtcgc agcccgccaa cttcagctaa ccgttttgcg
    10681 atttcaatca agactctatc gcccgtcgca tgaccccagg tatcattgat gtatttgaaa
    10741 ttatcgccat caagaaatag taacgccgac gtttttcggg catcggaatt gttcatcaac
    10801 gtgttgatgc cgctacgaaa cgctgcgcgg ttagccagcc cggttaatgg gtcatgtagc
    10861 gcggtacgta gaagctgcgc atttttagcc tgtaaacgaa gctgccactc ttccatttca
    10921 tccagcagac tgttgaagtc gagagcgaag cggtgaaact cagcgatacg ttcttccgaa
    10981 actcgtcggg aaaaattgcg gttggaacgc acatcatgta cgacatcggt gatatttttc
    11041 agtgcttcca ccaggccatt gtgcaaatgg cgggtgaggg taattgcgat gcctgatgcc
    11101 agcagaatac aaccggtcag tacggcgagc gaaaaccaga taaaatggct gattgaactg
    11161 tcgcgagcgg ttaagcgtac ttcgccaatg gtttcaccat tgtgacgaat cggctgaatg
    11221 atgggggcgg ggaagagcca gtggcttatg aaattgctga aagtatcgcc tggatcctta
    11281 cgggtgtaat gccaggacgc cagaatattt tgctgcttat cacgtacttc tgcagttgaa
    11341 aattgccctt gctggcccag cgctgccagt gtttcagttg ctgcagggcc atcggcaaaa
    11401 acgacagctg cttccagact gtaagtca
//
LOCUS       DADMKF010000128        11355 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.130, whole genome shotgun sequence.
ACCESSION   DADMKF010000128 DADMKF010000000
VERSION     DADMKF010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11355
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.130"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(<1..535)
                     /locus_tag="JNC24_003122"
     CDS             complement(<1..535)
                     /locus_tag="JNC24_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9159810.1"
                     /translation="MSQNDIIIRTHYKSPHRLHIDSDIPTPSSEPINQFARQLITLLD
                     TSDLSSMLSYCVTQEFTANCRKISQNCYSTALFIINFATSPIHAENILITLHYKKEII
                     PLFLETTPIKANHLRSILDYIEQEQLTAEDRNHCMKLSKKIHREKTIQPTVNLNGSAF
                     FSQSPSDAIFCRHLSLQY"
     gene            1060..2988
                     /gene="thrS"
                     /locus_tag="JNC24_003123"
     CDS             1060..2988
                     /gene="thrS"
                     /locus_tag="JNC24_003123"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010245687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HAY9159811.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            2992..3534
                     /gene="infC"
                     /locus_tag="JNC24_003124"
     CDS             2992..3534
                     /gene="infC"
                     /locus_tag="JNC24_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HAY9159812.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPGT
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            3631..3828
                     /gene="rpmI"
                     /locus_tag="JNC24_003125"
     CDS             3631..3828
                     /gene="rpmI"
                     /locus_tag="JNC24_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HAY9159813.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            3881..4237
                     /gene="rplT"
                     /locus_tag="JNC24_003126"
     CDS             3881..4237
                     /gene="rplT"
                     /locus_tag="JNC24_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016169860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HAY9159814.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
     gene            4360..4404
                     /locus_tag="JNC24_003127"
     CDS             4360..4404
                     /locus_tag="JNC24_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl--tRNA ligase operon leader peptide"
                     /protein_id="HAY9159815.1"
                     /translation="MNAAIFRFFFYFST"
     gene            4687..5670
                     /gene="pheS"
                     /locus_tag="JNC24_003128"
     CDS             4687..5670
                     /gene="pheS"
                     /locus_tag="JNC24_003128"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HAY9159816.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLLGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DTTRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            5685..8072
                     /gene="pheT"
                     /locus_tag="JNC24_003129"
     CDS             5685..8072
                     /gene="pheT"
                     /locus_tag="JNC24_003129"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012905611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HAY9159817.1"
                     /translation="MKFSELWLREWVNPAIDSDALANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPALQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLPSPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            8077..8376
                     /gene="ihfA"
                     /locus_tag="JNC24_003130"
     CDS             8077..8376
                     /gene="ihfA"
                     /locus_tag="JNC24_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HAY9159818.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            8477..9457
                     /gene="btuC"
                     /locus_tag="JNC24_003131"
     CDS             8477..9457
                     /gene="btuC"
                     /locus_tag="JNC24_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HAY9159819.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASAVLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            9520..10071
                     /gene="btuE"
                     /locus_tag="JNC24_003132"
     CDS             9520..10071
                     /gene="btuE"
                     /locus_tag="JNC24_003132"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HAY9159820.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGLVIQ
                     RFSPDMTPENPIVMESIKLALAK"
     gene            10071..10820
                     /gene="btuD"
                     /locus_tag="JNC24_003133"
     CDS             10071..10820
                     /gene="btuD"
                     /locus_tag="JNC24_003133"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HAY9159821.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            10898..>11355
                     /locus_tag="JNC24_003134"
     CDS             10898..>11355
                     /locus_tag="JNC24_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase"
                     /protein_id="HAY9159822.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLQLPRETKEQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARR"
BASE COUNT         2872 a         2631 c         3056 g         2796 t
ORIGIN      
        1 cgtattgcaa tgacagatgg cgacaaaaaa tagcgtcaga aggagattgc gaaaaaaatg
       61 cactaccatt gagatttact gttggttgta tagttttttc tctatggatt tttttagaca
      121 gtttcataca atggttacga tcttcggcag ttaactgttc ctgttcaata taatccagta
      181 tgcttcgcaa atggttagcc ttaataggcg tggtttccag aaataaggga atgatttctt
      241 ttttatagtg taatgtaatg agtatatttt ctgcatggat gggtgaagtg gcaaagttaa
      301 tgataaaaag ggcagtggaa taacaatttt gtgaaatttt tcgacagttt gcggtaaatt
      361 cctgagtaac acagtatgac agcatcgaac ttaagtcaga ggtatcaagt agggtgatga
      421 gttggcgcgc aaattgatta ataggctctg atgaaggcgt tggtatgtcg ctatcgatgt
      481 gcaatctatg aggagactta taatgggttc tgataatgat atcgttttga ctcataatct
      541 attcgcctta tatatgtctg gaggttttga ggatgatgat taattattat cgccagaacg
      601 aaatgtgcac gaaaaattgc tataagaatt gtgtcacttt tatcattgtg atatgaccac
      661 ttcgtcatta atctcatgag cgtttaaaat actaccgcca aatcacgcaa cgaactttgc
      721 tggattcagt ttatgctgct gtaaatccgc tcgcagtaaa cctttcagac gcaccgtgat
      781 gttatcagtg gttctttaag cgttttgctg gtgtactcac tacaaacgaa ttgcgaatcg
      841 aatcaatgtg aaacggaaag gtacaatctc ccttttttta gtgttaacat cgttcaaccg
      901 ggtggaaggt gagaggttaa tgtctttcat ttgtgtacct gattgcgaac caatttagca
      961 tttgttggct agatggtttc gcaatgaact gttaataaac aaatttttct ttgtatgtga
     1021 tctttcgtgt gggtcaccac tgcaaattaa ggatataaaa tgcctgttat aactcttcct
     1081 gatggcagcc aacgccatta cgaccacgct gtaagcccca tggatgttgc gctggacatt
     1141 ggtccaggtc tggcgaaagc ctgtatcgca gggcgcgtta atggcgaact ggttgatgct
     1201 tgcgatctga ttgaaaacga cgcacaactg tcgatcatta ccgccaaaga cgaagaaggt
     1261 ctggagatca ttcgtcactc ctgtgcgcac ctgttagggc atgcgattaa acaactttgg
     1321 ccgcatacca aaatggcaat cggcccggtt attgacaacg gtttttatta cgacgttgat
     1381 cttgaccgca cgttaaccca ggaagatgtc gaagcactcg agaagcggat gcatgagctt
     1441 gctgagaaaa actacgacgt cattaagaag aaagtcagct ggcacgaagc gcgtgaaact
     1501 ttcgccaacc gtggggagag ctacaaagtc tccattcttg acgaaaacat cgcccatgat
     1561 gacaagccag gtctgtactt ccatgaagaa tatgtcgata tgtgccgcgg tccgcacgta
     1621 ccgaacatgc gtttctgcca tcatttcaaa ctaatgaaaa cggcaggggc ttactggcgt
     1681 ggcgacagca acaacaaaat gttgcaacgt atttacggta cggcgtgggc agacaaaaaa
     1741 gcacttaacg cttacctgca gcgcctggaa gaagccgcga aacgcgacca ccgtaaaatc
     1801 ggtaaacagc tcgacctgta ccatatgcag gaagaagcgc cgggtatggt attctggcac
     1861 aacgacggct ggaccatctt ccgtgaactg gaagtgtttg ttcgttctaa actgaaagag
     1921 taccagtatc aggaagttaa aggtccgttc atgatggacc gtgtcctgtg ggaaaaaacc
     1981 ggtcactggg acaactacaa agatgcaatg ttcaccacat cttctgagaa ccgtgaatac
     2041 tgcattaagc cgatgaactg cccgggtcac gtacaaattt tcaaccaggg gctgaagtct
     2101 tatcgcgatc tgccgctgcg tatggccgag tttggtagct gccaccgtaa cgagccgtca
     2161 ggttcgctgc atggcctgat gcgcgtgcgt ggatttaccc aggatgacgc gcatatcttc
     2221 tgtactgaag aacaaattcg cgatgaagtt aacggatgta tccgtttagt ctatgatatg
     2281 tacagcactt ttggcttcga gaagatcgtc gtcaaactct ccactcgtcc tgaaaaacgt
     2341 attggcagcg acgaaatgtg ggatcgtgct gaggcggacc tggcggttgc gctggaagaa
     2401 aacaacatcc cgtttgaata tcaactgggt gaaggcgctt tctacggtcc gaaaattgaa
     2461 tttaccctgt atgactgcct cgatcgtgca tggcagtgcg gtacagtaca gctggacttc
     2521 tccttgccgt ctcgtctgag cgcctcctat gtgggcgaag acaacgagcg taaggtaccg
     2581 gtaatgattc accgcgcaat tctggggtcg atggaacgtt tcatcggtat cctgaccgaa
     2641 gaattcgctg gtttcttccc gacctggctt gcgccagttc aggttgttat catgaatatt
     2701 accgattcac agtctgaata cgttaacgaa ttgacgcaaa aactatcaaa tgcgggcatt
     2761 cgtgttaaag cagacttgag aaatgagaag attggcttta aaatccgcga gcacactttg
     2821 cgtcgcgtcc catatatgtt ggtctgtggt gataaagagg tggaatcagg caaagttgcc
     2881 gttcgcaccc gccgtggtaa agacctggga agcatggacg taaatgaagt gatcgagaag
     2941 ctgcaacaag agattcgcag ccgcagtctt aaacaattgg aggaataagg tattaaaggc
     3001 ggaaaacgag ttcaaacggc gcgccctaac cgtatcaatg gcgaaattcg cgcccaggaa
     3061 gttcgcttaa caggtctgga aggcgagcag cttggtattg tgagtctgag agaagctctg
     3121 gagaaagcag aagaagccgg agtagactta gtcgagatca gccctaacgc cgagccgccg
     3181 gtttgtcgta taatggatta cggcaaattc ctctatgaaa agagcaagtc ttctaaggaa
     3241 cagaagaaaa agcaaaaagt tatccaggtt aaggaaatta aattccgtcc tggtacagat
     3301 gaaggcgact atcaggtaaa actccgcagc ctgattcgct ttctcgaaga gggtgataaa
     3361 gccaaaatca cgctgcgttt ccgcggtcgt gagatggcgc accagcaaat cggtatggaa
     3421 gtgcttaatc gcgtgaaaga cgatttgcaa gaactggcag tggtcgaatc cttcccaacg
     3481 aagatcgaag gccgccagat gatcatggtg ctcgctccta agaagaaaca gtaaggcctt
     3541 caagtagcac tgtctgtgga gccttcgggc ttcacaggct ttgttcgcct ttgtttcgtt
     3601 tattaacaat gcgaagtgga agttattaaa atgccaaaaa ttaagaccgt acgcggtgct
     3661 gctaagcgct tcaaaaaaac cggtaaaggt ggttttaagc acaagcacgc taacctgcgt
     3721 cacattctga ccaaaaaagc gaccaaacgt aaacgtcacc tgcgtccgaa agccatggtt
     3781 tccaaaggcg atctgggcct ggtaatcgcg tgcctgccgt acgcataagc cgttaacgtt
     3841 tttaactttt taattagaat atagatacag gagagcacat atggctcgcg taaaacgtgg
     3901 tgttattgca cgcgcacgtc acaagaaaat tttgaaacaa gctaaaggct actacggtgc
     3961 gcgttctcgc gtataccgcg ttgccttcca ggctgttatc aaagctggtc agtatgctta
     4021 ccgtgaccgt cgtcaacgta agcgtcagtt ccgtcaactg tggattgcgc gtatcaacgc
     4081 agcagcacgt cagaacggta tttcttacag caaattcatc aatggcctga aaaaagcctc
     4141 tgttgaaatc gaccgtaaga tcctggctga tatcgcagta ttcgacaaag tagcgttcac
     4201 cgctctggtt gaaaaagcga aagcagctct ggcataagcc agttgaaaga gggagctagt
     4261 ctccctcttt tcgtttcaac gccatcaaaa cattgacttt tatcgccgta gccttttcaa
     4321 taaaggtctt ttgaagagta ccaaaaggta acgcaagcaa tgaatgctgc tattttccgc
     4381 ttcttttttt actttagcac ctgaatccag gaggctagcg cgtgagaaga gaaacggaaa
     4441 acagcgcctg aaagcctccc agtggaggct ttttttgtat gcgcgttttg aaaaataaaa
     4501 agccagtcta tcaggcgtaa ttgtcgcagc cagtctggac acggccagcg cggagaaacc
     4561 ggagcgtatt taagtacgtg agaatttcga gcactgcccg ggaccaaaat ggcaagtaaa
     4621 atagcctgat gggataggct ctaagtccaa cgaaccagtg tcaccactga cacaatgagg
     4681 aaaaccatgt cacatctcgc agaactggtt gccagtgcga aggcggccat tagccaggcg
     4741 tcagatgttg ccgcgttaga taatgtgcgc gtcgaatatt tgggtaaaaa agggcactta
     4801 acccttcaga tgacgaccct gcgtgagctg ccgccagaag agcgtccggc agctggtgcg
     4861 gttatcaacg aagcgaaaga gcaggttcag caggcgctga atgcgcgtaa agcggaactg
     4921 gaaagcgcag cgctgaatgc gcgtctggcg gcggaaacga ttgatgtctc cctgctaggt
     4981 cgtcgcattg aaaacggcgg tctgcatccg gttacccgta ccatcgaccg tatcgaaagt
     5041 ttcttcggtg agcttggctt taccgtggca accgggccgg aaatcgaaga cgattatcat
     5101 aacttcgatg ctctgaacat tcctggtcac cacccggcgc gcgctgacca cgacactttc
     5161 tggtttgaca ctacccgcct gctgcgtacc cagacctctg gcgtacagat ccgcaccatg
     5221 aaagcccagc agccaccgat tcgtatcatc gcgcctggcc gcgtttatcg taacgactac
     5281 gaccagactc acacgccgat gttccatcag atggaaggtc tgattgttga taccaacatc
     5341 agctttacca acctgaaagg cactctgcac gatttcctgc gtaacttctt tgaggaagat
     5401 ttgcagattc gcttccgtcc ttcctacttc ccgttcaccg agccttctgc agaagttgac
     5461 gtcatgggta aaaacggtaa atggctggaa gtgctgggtt gcgggatggt gcatccgaac
     5521 gtgctgcgta acgttggcat cgacccggaa gtttactctg gtttcgcctt cgggatgggg
     5581 atggagcgtc tgactatgtt gcgttacggc gtcaccgacc tgcgttcatt cttcgaaaac
     5641 gatctgcgtt tcctcaaaca gtttaaataa ggcaggaata gattatgaaa ttcagtgaac
     5701 tgtggttacg cgaatgggtg aacccggcga ttgatagcga tgcgctggcg aatcaaatca
     5761 ctatggcggg cctggaagtt gacggtgtag aaccggttgc cggtagcttc cacggcgtgg
     5821 tcgttggtga agtggttgag tgtgcgcagc atccgaacgc tgacaaactg cgtgtgacaa
     5881 aagtgaatgt cggcggcgat cgcctgctgg acatcgtctg tggtgcgcca aactgccgtc
     5941 agggcctgcg tgtggcggta gctaccattg gtgctgttct gccgggtgat ttcaaaatta
     6001 aagcggcgaa actgcgtggc gaaccgtctg aagggatgct gtgctccttc tctgaactgg
     6061 gaatttctga cgatcacagc ggcattatcg aactgcctgc agatgcgccg attggcactg
     6121 acatccgcga atacctgaaa ctcgatgaca acaccatcga aatcagcgta acgccaaacc
     6181 gtgccgactg cttaggtatc attggtgttg cgcgtgacgt tgccgtgctg aatcagctgc
     6241 cgctggttga accggaaatc gttccggttg gtgcgaccat cgacgacacg ttgccgatta
     6301 cagtcgaagc gcctgaagcc tgcccgcgct atcttggccg tgtggtaaaa ggcattaacg
     6361 ttaaagcgcc aactccgctg tggatgaaag aaaaactgcg tcgttgcggg atccgttcta
     6421 tcgatgcagt tgttgacgtc accaactatg tactgctcga actgggccag ccgatgcacg
     6481 ctttcgataa agatcgcatt gaaggcggca ttgtggtgcg gatggcaaaa gagggcgaaa
     6541 cgctggtgct gcttgatggc actgaagcga agctgaatgc tgacactctg gtcatcgccg
     6601 accacaacaa ggcgctggcg atgggcggca tctttggtgg cgaacactct ggcgtgaatg
     6661 acgaaacgca aaacgtcttg ctggaatgcg ctttcttcag cccgctgtct atcaccggtc
     6721 gtgctcgtcg tcatggcctg catactgatg cgtctcaccg ttatgagcgt ggcgttgatc
     6781 cggcactgca gcacaaagcg atggaacgtg cgacccgtct gctgatcgac atctgcggtg
     6841 gtgaggctgg tccggtaatt gatatcacca acgaagcaac gctgccgaag cgtgcaacca
     6901 tcactttacg tcgtagcaaa ctggatcgcc tgatcggcca tcatattgct gatgaacagg
     6961 taactgacat tctgcgtcgt ctcggctgcg aagtgaccga aggcaaagac gagtggcagg
     7021 cagttgcgcc gagctggcgt ttcgatatgg agattgaaga agatctggtt gaagaagtcg
     7081 cgcgtgttta cggctacaac aacatcccgg atgagccggt acaggcaagc ctgattatgg
     7141 gtactcaccg tgaagctgac ctgtcgctca agcgcgtgaa aacgctgctc aacgacaaag
     7201 gctatcagga agtgatcacc tacagcttcg ttgatccgaa agtgcagcag atgatccatc
     7261 caggcgttga agccttactg ctgccaagcc cgatctctgt tgaaatgtca gcaatgcgtc
     7321 tttctctgtg gaccggcctg ctggcaaccg tggtgtacaa ccagaaccgt cagcagaacc
     7381 gtgtgcgcat tttcgaaagc ggtctgcgtt tcgtaccaga tactcaggca ccgttgggca
     7441 ttcgtcagga tctgatgtta gccggtgtga tttgcggtaa ccgttacgaa gagcactgga
     7501 acctggcaaa agagaccgtt gatttctatg atttgaaagg cgatcttgaa tccgttctcg
     7561 acctgaccgg taaactgaat gaggttgagt tccgtgcaga agcgaatccg gcactgcatc
     7621 cggggcaatc cgcagcgatt tatctgaaag gggaacgtat tggttttgtt ggagttgttc
     7681 atcctgaact ggaacgtaaa ctggatctta acggtcgcac tctggtgttc gaactggagt
     7741 ggaacaagct cgcagaccgc gtggtgcctc aggcgcgcga gatttctcgc ttcccggcga
     7801 accgtcgtga catcgcggtg gtggtcgcag aaaacgttcc cgcagcggat attttatccg
     7861 aatgtaagaa agttggcgta aatcaggtag ttggcgtaaa cttatttgac gtgtaccgcg
     7921 gtaagggtgt tgcggagggg tataagagcc tcgccataag cctgatcctg caagatacca
     7981 gccgtacact cgaagaagag gagattgccg ctaccgtcgc caaatgtgta gaggcattaa
     8041 aagagcgatt ccaggcatca ttgagggatt gaacctatgg cgcttacaaa agctgaaatg
     8101 tcagaatatc tgtttgataa gcttgggctt agcaagcggg atgccaaaga actggttgaa
     8161 ctgtttttcg aagagatccg tcgcgctctg gaaaacggcg aacaggtgaa actctctggt
     8221 tttggtaact tcgatctgcg tgataagaat caacgcccgg gacgtaaccc gaaaacgggc
     8281 gaggatattc ccattacagc acggcgcgtg gtgaccttca gacccgggca gaagttaaaa
     8341 agccgggtcg aaaacgcttc gcccaaagac gagtaatctg atctaactaa aaaggccgct
     8401 ctgcggcctt ttttcttttc actgtcgaag agtcaccgta aaatcaacgc catgacactt
     8461 cagcagaacg gataccatgc tgacacttgc ccgccaacaa cagcgacaaa atattcgctg
     8521 gttattatgc ctgtcagttt tgatgctgct ggcgcttctc ttaagccttt gcgccggtga
     8581 acaatggatt tcgccaggtg actggtttac tcctcgtggc gaactgttcg tctggcaaat
     8641 tcgcctgcca cgtacgctgg ctgtattgct ggttggtgcg gcgctggcta tatccggcgc
     8701 tgtaatgcag gcgttgtttg aaaatcctct ggcagaacct ggactacttg gcgtctctaa
     8761 cggcgcaggc gtggggctta tcgccgcggt attgcttggg caagggcaac tccccaactg
     8821 ggcgctaggg ctgtgtgcga ttgctggcgc gcttatcatc actttaatac tcttacgttt
     8881 cgcccgtcgt catctttcga ccagtcggtt attgctggct ggcgttgcat tagggattat
     8941 ctgtagcgca ctaatgacgt gggctatcta cttttccacc tctgttgatt tacgtcagct
     9001 gatgtactgg atgatgggcg gttttggcgg cgtagactgg cggcaaagct ggctgatgct
     9061 ggcattgatc cccgtgttgt tgtggatctg ttgtcagtcc aggccgatga atatgttagc
     9121 acttggcgag atctcggcgc ggcaactggg tttacccctg tggttctggc gcaatgtgct
     9181 ggtggcagcg accggctgga tggttggcgt cagtgtggcg ctggcgggtg ctatcggctt
     9241 tattggtctg gtgatcccac atattcttcg gttgtgtggt ttaaccgatc atcgcgtatt
     9301 acttcccggc tgcgcgctgg caggggcgag cgcagtgctg ctggccgata ttgtagcgcg
     9361 cctggcatta gctgccgcag agctgcctat tggcgtggtc accgcaacgt tgggtgcgcc
     9421 ggtgtttatc tggttattgt taaaagcagg acgttagccg caaaaagacg gtctatgatt
     9481 aaaagctaag atttttactg accacaccca ggagaaacga tgcaagattc cattctgacg
     9541 accgtagtga aagatatcga cggtgaagtg accacgctgg agaagtacgc cggtaatgtg
     9601 ctgttgattg tcaatgtcgc ctcaaagtgt ggcttaacgc cgcaatatga gcagttggag
     9661 aatattcaga aagcctgggc cgatcgaggt tttgtggtgc tgggattccc gtgcaaccag
     9721 tttctggaac aagaaccggg cagcgatgaa gagattaaaa cttactgtac caccacatgg
     9781 ggggtgacgt tcccgatgtt cagtaagatt gaagttaacg gcgaaggacg ccatccgctg
     9841 tatcaaaaat tgattgccgc agcgccgacc gcagtcgcgc cggaagagag cggattctat
     9901 gcccgtatgg tcagcaaagg tcgtgcaccg ctgtaccctg atgatatttt atggaatttt
     9961 gagaaatttt tagttggcag ggacggattg gtcatccagc gtttttcccc ggatatgacg
    10021 ccggaaaacc ccattgtgat ggaaagcatt aaactggcgt tggcaaaata atgtctattg
    10081 tgatgcagtt acaggatgtt gcggaatcta cccgcctagg gccactttct ggcgaggttc
    10141 gggctgggga gatcctgcac ctggtggggc cgaatggcgc gggtaagagt accttactgg
    10201 cacgaatggc cggaatgacc agcggtaagg gaagtattca gttcgcgggg caaccactgg
    10261 aagcatggtc cgcaacaaaa ctcgcgctgc atcgcgccta tctttcacaa cagcagacgc
    10321 cgccgtttgc aatgccggtc tggcactatc tgacactgca tcagcacgat aaaacgcgta
    10381 ccgaactact gaatgatgtc gcaggggcgc tggctcttga tgacaaactc ggacgtagca
    10441 ccaatcaact ttccggcggt gaatggcaac gcgtacgtct tgctgcggtg gtgctgcaaa
    10501 tcacaccaca agccaatccc gcaggccaat tgctgcttct tgatgagccg atgaacagtc
    10561 ttgatgttgc gcaacaaagt gcgttagaca aaattctgag cgctctgtgt cagcaaggac
    10621 tggcgattgt gatgagcagt cacgatctca accacacatt gcgtcatgcg catcgggcgt
    10681 ggttgctaaa aggtggaaaa atgctggcca gtggacgcag agaagaggtg ctcacgccgc
    10741 caaatctggc gcaggcctat gggatgaatt ttcgccgtct ggatatcgaa ggtcacagaa
    10801 tgctgatttc gaccatctga tttcattagt atcttgaaaa tcgggtaatt acgacgctaa
    10861 attaatgcaa gaataaaaaa cagaggaacc gtacggaatg cgtttctgcc ttattttgat
    10921 cacagcactg tttctggccg ggtgtagcca ccataaagca ccgccgccaa atgccagact
    10981 ttctgattcg attaccgtta ttgccggttt gaacgaccag ctacaaagct ggcatggcac
    11041 gccgtatcgt tatggtggca tgacgcggcg cggtgtggac tgttcgggat ttgtggttgt
    11101 aacgatgcgc gatcgtttcg atttgcagct gccccgagaa accaaagaac aagcctctat
    11161 cggcacgcaa attgataaag acgagttgct gcctggtgac ctggtctttt tcaaaacggg
    11221 ttccggacaa aatggtttgc atgtaggtat ttacgatacc aacaaccaat ttatccacgc
    11281 ctctaccagc aagggagtga tgcgttcctc acttgataat gtctattggc agaaaaattt
    11341 ctggcaggcg agacg
//
LOCUS       DADMKF010000129        11269 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.131, whole genome shotgun sequence.
ACCESSION   DADMKF010000129 DADMKF010000000
VERSION     DADMKF010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11269
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.131"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            <1..210
                     /locus_tag="JNC24_003135"
     CDS             <1..210
                     /locus_tag="JNC24_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY9159823.1"
                     /translation="KIVPLTVTWGGEEIKADAATTFTATKIFASDALTNGSLAKNLMF
                     AQTTKGVLETGIYRGVVSIYLSQDI"
     gene            302..2986
                     /locus_tag="JNC24_003136"
     CDS             302..2986
                     /locus_tag="JNC24_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY9159824.1"
                     /translation="MDKKLLALLILASLSPAEATLTEIPAGFEVIAQGQQEYIEVYFS
                     GKSLGKYYAMVNLDTVTFLDPASLYNKLELDVDDQKIAHIVKEKLSQPLAHHGELACG
                     YVRTDSGCGFLNTDTLEIIYNDEESSATLFINPQWNSAFDAKSLYLNPDKNTVNAFIH
                     QQDINVLAQDDYQSLSIQGNGALGITENSYIGAHWNFNGYDADDVSDSNADVSDLYYR
                     YDFLRRYYVQAGRMDNRTLFNAQGGNFTFNFLPLGAIDGMRIGSTLSYLNQAQSQQGT
                     PVMVLLSRNSRVDAYRNEQLLGSFYLNSGSQFIDTSSFPPGSYSVALKVYENNQLTRT
                     ELVPFTKTGGLTDGNAQWFLQAGKTTSQVSDDESSAYQLGVRLPLHPQYELYAGLANA
                     DDVSAFELGNNWTADLGGVGNLAISASVFRNDDGGKGDMQQANWSNPGWPTLGFYRTN
                     SDGDACTTDSRESYNALSCYESISATVSQNFVGWNMMLGYTRTQNNTDDSLRWDKQQS
                     FENNYLRQTTAQSISETVQLSASRAFVMRDWILSTSVGVFHRNDNGGDNDDNGLYLSF
                     SLSDTPTMDSNNNSHSTNVSTDYRYSEQDGDQTSWQLSHTFYNDSFSHKELGVTVGGL
                     NTDTINSAVNGRWDGQYGNVYATVSDSYDRKNHDHLSAFTGTYSSTLAVSCYGVNLGA
                     SGTDDLLGAVLVDVKGFSEQDEESQDLQLEARVAGSRTLQLGQSDSVLFPYPGFQSGF
                     VEVNDSSQGNQQGTTNIINGAGNRELMLLPGKLRYREVSASFNYNYIGRLLLPAAVKK
                     FPIVGLNSAMLLVAEDGGFTLEINGSEKELYLLSGQQFLKCPLSVVKKRASIRYSGDV
                     TCSVVTYSQLPESIQVQAQLKQPKLRGNVQTAQREVAP"
     gene            3067..4071
                     /locus_tag="JNC24_003137"
     CDS             3067..4071
                     /locus_tag="JNC24_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein CblD"
                     /protein_id="HAY9159825.1"
                     /translation="MTYDLASGRADYYFWKDEASAGNNGYMWYECSYPDLQQTCTANG
                     NISTVQIYLTEQRSGMRWPVKLKGFKTAIVSSDEAPPGCKGGKGLQTNLKDSNRSSCT
                     EDGQHYYIYDTKFLTLYLEQTEMKNLPIGGVWKGKVKLHSNSPAQDYFANITLNTLDP
                     NHIDVFFPEFAHATPRVQLDLHPTGSVNGSNYAQDLTMLDMCLYDGFNGNAISYEIML
                     KDEGRPAAGRRDGDFSIYRQGGTTTDEGERIDYRVKMYNPETGGQIDVRNNENMVWNS
                     INLKRVRPVVLPGIRYAVMCVPTPLTLAVDKFSVMDKQAGYYMGKLSVIFTPSLPTIN
                     "
     gene            complement(4154..5451)
                     /locus_tag="JNC24_003138"
                     /pseudo
     CDS             complement(4154..5451)
                     /locus_tag="JNC24_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            5934..7346
                     /gene="uxaC"
                     /locus_tag="JNC24_003139"
     CDS             5934..7346
                     /gene="uxaC"
                     /locus_tag="JNC24_003139"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAY9159826.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            7361..8848
                     /locus_tag="JNC24_003140"
     CDS             7361..8848
                     /locus_tag="JNC24_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HAY9159827.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADREVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINIRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            8931..9482
                     /locus_tag="JNC24_003141"
     CDS             8931..9482
                     /locus_tag="JNC24_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HAY9159828.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAVDERG"
     gene            complement(9487..10731)
                     /gene="sstT"
                     /locus_tag="JNC24_003142"
     CDS             complement(9487..10731)
                     /gene="sstT"
                     /locus_tag="JNC24_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HAY9159829.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
BASE COUNT         2963 a         2754 c         2925 g         2627 t
ORIGIN      
        1 aagattgttc cgctgacagt aacctggggc ggtgaagaaa ttaaagctga tgcggcaacc
       61 acttttaccg caaccaaaat ctttgcttcc gatgcgttaa ctaacggttc tctggctaaa
      121 aacctgatgt ttgctcaaac caccaaaggt gttctggaga caggcatcta ccgtggtgta
      181 gtaagcattt atctgtctca ggatatctaa tccagataac agaattaaca cgtaacaaag
      241 ggaggggtag ccctccctta ttttaactgt tttagtattt gttatggatt tcaggattgc
      301 catggataaa aaattactgg ctcttttgat cctggcgagt ctcagcccgg cagaggcgac
      361 attaaccgaa attcccgcag ggtttgaggt tattgctcag ggacagcagg agtatatcga
      421 ggtttatttt tcagggaaaa gtctcggtaa atattatgca atggttaatc ttgataccgt
      481 aacatttctt gatccagcaa gtctatataa caagctggaa ctggatgtag acgatcagaa
      541 aatcgcgcat atagtgaaag aaaaattatc gcagccgcta gctcaccacg gtgaattggc
      601 ttgcggttat gtacgtactg actcagggtg tggttttctg aataccgata cgctggaaat
      661 aatctataat gatgaagaaa gttcggcaac gttgtttatt aatccgcaat ggaattcagc
      721 tttcgatgcg aagtcattat atttaaatcc agacaaaaat actgttaatg cttttataca
      781 tcagcaagac atcaatgttc tggcacagga tgattaccaa tcgttgtcta ttcagggaaa
      841 cggtgcgctg ggaataacag aaaatagtta tattggtgca cactggaatt tcaacggtta
      901 tgatgcagat gatgtcagtg acagtaatgc tgacgtcagc gatctctatt atcgttatga
      961 ttttttacgt cgttattatg tgcaggcggg tcgcatggac aaccgcacac tatttaatgc
     1021 acaaggcggg aactttacct ttaactttct gccactcggt gcaattgatg ggatgcgtat
     1081 cgggtcgaca ctgagctatt taaaccaggc gcaaagccag caaggaaccc cagtaatggt
     1141 tctgctttcg cgcaattctc gtgttgacgc ttatcgtaat gagcaacttt tgggatcgtt
     1201 ttatctcaat agtggttcgc aatttattga taccagttcc tttccgccag gcagctacag
     1261 cgtggcatta aaagtctacg aaaataacca actcacccgc accgagcttg tgccgtttac
     1321 caaaaccggc ggtctgactg acggaaatgc gcaatggttc ttacaggcag gtaaaactac
     1381 atcacaggtt tctgatgatg aaagttcagc ttatcagcta ggagtacgcc tgccattaca
     1441 tccgcaatat gagctctacg cagggctggc gaatgccgat gatgtgagtg ctttcgagtt
     1501 aggtaataac tggacggcag atttaggcgg ggtggggaat cttgcaatca gcgccagcgt
     1561 gttccgtaac gatgacggcg gcaaaggtga tatgcaacag gccaactgga gtaatccggg
     1621 atggccgacg ttgggctttt atcggaccaa ctctgacggc gatgcttgta caaccgacag
     1681 cagagagagc tataacgcct taagctgtta tgaaagtatt tccgcgacgg tttcacagaa
     1741 ttttgtcggc tggaatatga tgctgggtta tacccgcaca caaaataaca ctgatgatag
     1801 tttgcgttgg gataaacagc agagctttga aaataactat cttcgccaga caacagcgca
     1861 aagtatctct gaaactgtac aacttagcgc ttcccgcgct tttgtgatgc gtgactggat
     1921 tttgagtacg tcagtcggtg ttttccatcg taatgacaac ggtggcgata acgacgacaa
     1981 cggcttgtac ttatcgtttt cgttatctga cacgccaacg atggatagca ataacaacag
     2041 ccattcaaca aatgtgtcta cggattatcg ttatagcgaa caggatggcg atcaaacgtc
     2101 atggcagtta tcgcatacct tttataacga ttcattcagc cataaagaac ttggcgtaac
     2161 cgtcggaggc ctgaacaccg ataccataaa cagcgcggtt aacgggcgtt gggatgggca
     2221 atacggaaat gtctacgcta ccgtatctga tagttatgac cgtaagaatc atgatcatct
     2281 ttcggccttt acgggcactt atagctctac gctggctgtc agttgctatg gcgttaattt
     2341 gggagccagt ggtacagacg atttgctggg tgcggtactg gtggatgtga aaggcttctc
     2401 tgaacaggat gaagagagtc aggatctgca actcgaagcg cgggtggcag gcagccgaac
     2461 tttgcagctt ggtcaaagtg acagtgtgtt gttcccttat cctggatttc agtctggttt
     2521 tgttgaggtt aacgacagta gccagggcaa tcagcagggg acaacaaaca tcattaacgg
     2581 tgcagggaat cgtgaattaa tgttgttgcc tggcaagctg cgctatcgcg aagtgtctgc
     2641 cagctttaac tacaactata tcggtcgttt attactgccg gcagcggtga aaaaattccc
     2701 gattgtgggg ctgaacagcg ccatgttact ggtagctgaa gatggaggat ttacacttga
     2761 aattaacggc agcgaaaaag agctctatct gctttccggg cagcaattcc ttaagtgtcc
     2821 actgagtgtt gtaaagaaac gcgccagcat tcgttacagc ggagatgtta cttgtagtgt
     2881 ggtgacttat tcacaattac cggagtcgat tcaggttcag gcacagttga aacagcctaa
     2941 attacgtgga aacgttcaga cggcgcaaag ggaggttgca ccatgagaaa tcgattgatt
     3001 gcggcgatat tgggcttgtt tggcacgctc actggcgttc aggcagctcc tgacgtgacc
     3061 agtgaaatta cgtatgattt ggcatctggt agagcggatt attacttctg gaaggatgaa
     3121 gcgtcggcag gaaataatgg atatatgtgg tatgaatgtt cgtatcctga cctccaacaa
     3181 acctgtacag ctaatggaaa tatatcgaca gtacaaatct atttaactga acaacgcagt
     3241 gggatgcgtt ggccggtaaa actcaaagga tttaaaacag ccattgtaag tagcgatgaa
     3301 gcgccgccag gatgcaaggg gggcaaaggg cttcagacga atcttaagga ttctaataga
     3361 tcttcatgta cagaagatgg tcaacattat tatatatacg atacaaagtt tcttacgctc
     3421 taccttgagc agacagagat gaagaatttg ccgattggtg gcgtctggaa ggggaaagtt
     3481 aaattacatt cgaacagccc ggcccaggac tatttcgcaa atattaccct gaatacgctc
     3541 gaccccaacc atattgacgt gttcttcccg gagttcgccc acgccacgcc aagggtgcag
     3601 ttagacttgc atccaacagg aagcgttaac ggcagcaact acgcgcaaga tctgaccatg
     3661 ttggacatgt gcctgtacga tggttttaac ggtaatgcca tcagttatga aatcatgctc
     3721 aaagatgaag ggcgaccagc tgcagggcgc agagacggtg acttctctat ctatcgtcaa
     3781 ggagggacca ccaccgacga gggagaacgc attgattacc gggtcaaaat gtacaacccg
     3841 gaaaccggtg ggcaaattga tgtgcgcaat aatgaaaata tggtctggaa cagcattaac
     3901 ctgaaacgtg tgcgtccggt ggtactgccc ggtattcgct atgccgtgat gtgtgtgccg
     3961 acacccttga ctctggcagt cgataaattc agcgtgatgg ataaacaggc cgggtactac
     4021 atgggcaaat tgtcagtaat ctttacgcct tccttgccaa ccatcaattg accccgcaga
     4081 ccagaaaaaa ctaaacaacg aagccgcctg ataaggcggc ttcgtcatta caacttacgt
     4141 taaacgttaa acgttaatgt tgcggtgcgg gatcgttatg tgtttcctgc gccacctcaa
     4201 tcgccggttt gttctgcaat acggtccaga taaccagcgc acccagcagg tcaaatactg
     4261 ccagaactgc gaacagcggg ctgaagccga tggtgtcagc cagtgcaccg acaaccagcg
     4321 caaacagcgt acttgccagc catgcggaca tcccggttaa accgttcgcc gtcgccactt
     4381 cgttacgacc gaacacgtca gaagagagcg taatcagcgc gccagacagt gcctggtggg
     4441 caaaaccacc gatacacagc agcataattg cgacatacgg gttggtgaac agaccgatca
     4501 tacccgggcc aatcatcagc acagcaccca gcgttacgac catcttacgg gaaacgatca
     4561 ggttcacacc aaaccaacgc tggaacagcg gcggcaggta accaccgagg atacaaccga
     4621 ggtcagcaaa cagcatcggc atccaggcga acatcgcgat ctctttcagg ttaaagccgt
     4681 aaactttaaa catgaacagc gggatccacg cgttaaaagt accccaggcc ggttctgcca
     4741 ggaaacgcgg cagtgcgata ccccagaact gacggttacg caggatctga ccaacggaca
     4801 ttttcttcgc cgtgctcacc tggtgctggg cttcctgacc attaataata tagtcgcgtt
     4861 cttcatcggt cagatgcttc tggtcgcgcg gatgtttata gaaaatcagc catgccatcg
     4921 cccagataaa gctcaatgca ccggagatga taaatgccat ctgccagctg tgcatcacga
     4981 ttgccatacc accagcggcg gcgcaatcat cgcaccaatc gaagaaccta cgttaaagta
     5041 gcctactgcg atggaacgct ctttcgccgg gaaccattcg gagctggctt tcagacccgc
     5101 cgggatcatc gctgcttccg cggcaccgac cgcaccacga gcaacagcca ggccacccca
     5161 gctacctgcc agcgcggttg caccacagaa cacggcccac agcacagcaa acattgcata
     5221 accgattttc gtacccagca catccagtac ataacctgct accggttgca tgaccgtata
     5281 agcagcagaa taggctgcga tgatatagga atactgttgg gtggagatgt ttaactcttc
     5341 catcagagtt ggcgcagctg ccgccacagt gttacgcgtc aggtaaccaa gcacggtgcc
     5401 gagcgtcacc agtgcgatca tataccaacg taacccttta attttacgca tgtaaaacct
     5461 catcgttttg ttatatccgc aggaagcgcg gccatcatcc cgccagaggc tgaagatgtt
     5521 agttacgacc gggtaaccga aaaaaaaccg gcacgccccg aacgttgcca tgaaagtttc
     5581 gtgctaattc ggcttccgta ccggttaacc cgattcatct aacaaatgaa tgaatcggca
     5641 atttgcattc cgtcgactta tgatttgcga cggcagaaag ataacttgtc atacaacttt
     5701 aaaaggtgag agccatcaca aatgtgcgaa tatttgtagg gacattacct gacgacagcg
     5761 aggccagtac tggcgcggcc tgcagcgaga tttatcactt tgagagtaat ttttttaact
     5821 acgtttattg atctaactca cgaaaatatc ttcggactct ggaaattggt gtgataactt
     5881 tgtcagcatc gcaccataag caagctagct cactcgttga gaggaagacg aaaatgactc
     5941 cgtttatgac tgaagatttc ctgttagata ccgaatttgc ccgccgtctg tatcacgact
     6001 acgcaaaaga ccagccgatt ttcgattacc attgccattt gccgccgcag cagattgcgg
     6061 aagactatcg ttttaaaaac ctgtatgaca tctggctgaa aggcgatcac tacaaatggc
     6121 gcgctatgcg taccaacggt gtggccgagc gtctgtgtac cggtgatgca tctgaccgtg
     6181 aaaaatttga cgcctgggcg gcgactgttc cgcacactat cggcaacccg ttataccact
     6241 ggacgcacct tgaactgcgt cgtccgtttg gtatcactgg caaattgctt tctccgtcaa
     6301 ctgccgatga aatctggaac gaatgtaacg aactgctggc gcaggataac ttctccgcgc
     6361 gcggcatcat gcagcagatg aacgtgaaaa tggtcggcac caccgatgac ccgatcgatt
     6421 ctctggagca tcacgcagag atcgccaaag acggctcttt caccatcaaa gtgctgccga
     6481 gctggcgtcc ggacaaagcc ttcaacatcg aacaggcgac ctttaacgac tacatggcga
     6541 agctgggcga agtttccgat accgacattc gccgctttgc tgacctgcaa actgccttga
     6601 ccaaacgtct ggatcacttc gccgctcacg gctgtaaagt gtctgaccat gcgctggacg
     6661 tggtgatgtt tgctgaagcg aacgaagcgg aactggacag cattctggcg cgccgtctgg
     6721 ctggcgaaac cctgagcgag cacgaagtgg cacagttcaa aactgcggta ctggtgttcc
     6781 ttggtgccga atatgcacgt cgcggctggg tacagcagta ccacattggc gcgctgcgta
     6841 ataacaacct gcgtcagttc aaactgctgg ggccggatgt aggctttgac tccatcaacg
     6901 accgtccgat ggcggaagag ttgtctaagc tgctgagcaa gcagaacgaa gaaaacctgc
     6961 tgccgaaaac cattctctac tgcctgaacc cgcgcgataa cgaagtgctg ggcaccatga
     7021 tcggtaactt ccagggcgaa ggtatgccgg gcaaaatgca gttcggttcc ggctggtggt
     7081 ttaacgatca gaaagacggt atggaacgtc agatgaccca actggcgcag ctcggtctgc
     7141 tgagccgctt tgtcggtatg ctgactgaca gccgtagctt cctgtcatac acccgtcacg
     7201 aatacttccg ccgcattctg tgccagatga tcggtcgctg ggtggaagcg ggcgaagcac
     7261 cggcggacat caacctgctg ggcgagatgg tgaaaaatat ttgctttaac aatgcgcgtg
     7321 actacttcgc cattgaactg aactaaggtc tggggttgat atgcaataca tcaagatcca
     7381 tgcgctggat aacgtcgcgg tcgctttagc agatttggct gaaggcacag aagtcagtgt
     7441 cgataaccag actgttacgc tgcgccagga tgttgctcgt ggacataaat ttgcgttaac
     7501 ggatatcgca aaaggggcca acgtcatcaa atatggcctg ccgattggtt atgcattggc
     7561 ggatattgcg gcgggggaac acgtccacgc ccacaatacg cgcacgaatc tgagcgatct
     7621 ggatcagtat cgctatcaac ctgattttca ggatctgcct gcgcaagcgg cagatcgtga
     7681 agtgcagatc tatcgtcgcg ctaacggcga tgtcggggtg cgtaacgagc tgtggatcct
     7741 gccaaccgtg ggctgtgtca acggcatcgc gcggcaaatc cagaaccgtt tcctgaaaga
     7801 gaccaacaac gccgaaggta ccgacggcgt gttcctcttc agccacacct acggctgctc
     7861 acaactgggc gacgatcaca tcaacatccg caccatgctg caaaacatgg tgcgccaccc
     7921 gaacgcgggc gcagtgctgg tgattggtct gggctgtgaa aacaaccagg ttgccgcatt
     7981 ccgtgaaacg ttgggcgata tcgatcctga acgcgttcat ttcatgatct gccaacagca
     8041 ggatgatgag atcgaagccg ggatcgagca tttgcatcag ctgtataacg tgatgcgcaa
     8101 cgataaacgc gagccaggca aactcagcga actgaagttt ggtctggagt gcggtggttc
     8161 tgacggtctt tctggtatta ctgctaaccc gatgctgggg cgtttctctg actacgtgat
     8221 tgctaacggc ggtactaccg tactgaccga agtgccggag atgtttggcg cagagcagtt
     8281 gctgatggac cattgccgcg acgaagcaac gtttgaaaaa ctggtcacca tggtcaacga
     8341 ctttaaacag tactttattg cccatgatca gccgatctac gaaaacccat caccggggaa
     8401 caaagcgggc ggtatcacca cgctggaaga caaatcactt ggctgtaccc agaaagcggg
     8461 ttccagtgtc gtggttgacg tgctgcgtta cggcgagcgt ctgaaaacgc ctgggctgaa
     8521 cttgttaagt gcgccgggta acgatgccgt agcgaccagc gccctggcgg gtgcgggctg
     8581 ccatatggtg ctgttcagta ctggtcgcgg cacgccatac ggtggatttg tgccgacggt
     8641 gaaaatcgcc accaacagtg aactggcggc gaagaaaaaa cactggatcg actttgacgc
     8701 gggtcagctg atccacggta aagcgatgcc gcagttgctg gaagaattta tcgacaccat
     8761 cgttgagttt gccaacggta agcaaacttg caacgagcgt aacgacttcc gcgaactggc
     8821 gatcttcaaa agcggcgtaa cgctataaag tcgaaatgag ttgttaataa aaaacggcgt
     8881 ttcataatgt gaagcgccgt ttgcttttcc atacctgata aggatttatc atgaccgcgt
     8941 attggctggc ccagggcgtg ggtgtcatcg cctttctgat tggtatcaca acatttttca
     9001 atcgtgacga acgtcgcttc aaaaagcagc tttcggtcta tagcgccgtt attggcgtac
     9061 atttttttct tctgggcacc tatcccgctg gtgccagtgc catccttaat gccattcgta
     9121 cattgattac cttacgcacg cgcagcttat gggtaatggc gatttttatt gtgctgactg
     9181 gcggaattgg cctcgcgaag ttccatcatc ctgtcgaact attgccggtt atcggtacga
     9241 ttgtcagtac ctgggcgctg ttccgctgta aagggctcac catgcgctgc gtgatgtggt
     9301 tttcaacgtg ttgctgggtg attcacaact tctgggcggg gtcgataggc ggcacaatga
     9361 ttgagggtag ttttctgctt atgaatggct tgaatatcat tcgtttctgg cggatgcaga
     9421 aaaggggaat tgatccgttt aaagtagaga aaacccctcc cgccgtagac gaaaggggtt
     9481 aaacaattaa ttacgcaggg cgctatttgc cagacgatcg tcttccgcct ggcaagctgc
     9541 cgcagtgaac agcacgtcag ttgaagagtt cagcgcggtt tcgcaagagt cctgcaatac
     9601 gccgatgata aagccgacgg caaccacctg catggcgata tcgttcgaaa taccgaacat
     9661 attacaggcc agcgggatca gcagcagaga accccccgcc acgccggatg cgccacaggc
     9721 acacagagaa gccaccacgc tcaacagcag cgccgtgggc agatcgaccg ggatacccag
     9781 cgtattaacc gcagccagcg tcaacacggt aatggtgatt gctgcgcccg ccatattgat
     9841 ggtggcaccc agcggaatag aaacggaata ggtatcgcga tccagattca gcttttcaca
     9901 cagtgccata ttcaccggaa tgttcgccgc agagctgcgg gtgaagaagg catacacgcc
     9961 gctttcgcgc aggcacagca gcaccagcgg gaacgggtta cgacgaattt tccaccacac
    10021 cagcaatggg ttaaccacca gcgccaccag taacatacag ccaaccagca cgaccagcag
    10081 ttgcgcgtag ccccacagtg tggagaaacc ggtggttgcc agggtagaag aaaccagacc
    10141 aaaaatacca atcggtgcga agcgaatgac cagtttcacc ataaaggtga cggcattcga
    10201 catatcgttg accaggtttt tggtggtctc gttaccgtga cgcagtgcga aaccgaggcc
    10261 aattgcccac accaggatcc cgatgtagtt acctttcagc agcgcgtcga tggggttgga
    10321 aaccatgctc attaccagcc cgcgcatcac ttctacaatg cctgacggtg gcgaaatatc
    10381 acccgcgcta ctggataagt gcagggtaga agggaaggcg aagctgaaaa cgactgcggc
    10441 cagagcagca gagaaggtac ccagcagata gaggaacaaa atagggcgga tattggtttt
    10501 ctgcccatgc tggtggttag caatagatgc catcaccagc atcaacacca ggatgggggc
    10561 aacggctttc agtgcgccga cgaacaaagt acctaacaga ccaacagctt ccgccgcggg
    10621 ttttgatatc catgccagaa gaatccccag aacaaggccg accaggattt gttttaccag
    10681 gctgccatga gccagacgcc ggaatagccc cggtgaacgt tgcgtagtca tttttcgatc
    10741 ctttcattgt gttatcgcac atccctggtg cgttctgtcg acgcatcttt caggatgtaa
    10801 caaagtgttt gcaaggtcga gtataaggaa agttacagct tcgggaagcg aaaaatgctg
    10861 gattttaaag agaagtcata ttttgaaact taatggttac atttgtttga cataaacaga
    10921 gggaaggact ggctactaag gctgattaga taacatatgg gcaaaactat ttggaatatg
    10981 agaacggttg cgctccttta atgtgctatg ccatataaag tcactggcgc agggagcgcg
    11041 cagggggcgg ccaatcgccg ccgcgcgatt ccctcagctt atcccttgcg gctaccgggt
    11101 cgggcgcaag ggaacgtccc tgtaagatgc gccctcgccg cgcatccatg cgcgtcgccc
    11161 cggcctccag ggaacgcttc ggcgattttg acgccaccaa cacccgtgcg gtctttttgt
    11221 aaagagctac aagccgatta cataattaat agctcacctt tgtctggaa
//
LOCUS       DADMKF010000130        11014 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.132, whole genome shotgun sequence.
ACCESSION   DADMKF010000130 DADMKF010000000
VERSION     DADMKF010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11014
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.132"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            122..883
                     /locus_tag="JNC24_003143"
     CDS             122..883
                     /locus_tag="JNC24_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAY9159830.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            1020..2357
                     /gene="adiC"
                     /locus_tag="JNC24_003144"
     CDS             1020..2357
                     /gene="adiC"
                     /locus_tag="JNC24_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HAY9159831.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            2461..4104
                     /gene="eptA"
                     /locus_tag="JNC24_003145"
     CDS             2461..4104
                     /gene="eptA"
                     /locus_tag="JNC24_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HAY9159832.1"
                     /translation="MLKRLLKSPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILVGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWLKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQQAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPGQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDVLQTCRRVSE"
     gene            4101..4769
                     /gene="basR"
                     /locus_tag="JNC24_003146"
     CDS             4101..4769
                     /gene="basR"
                     /locus_tag="JNC24_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HAY9159833.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYTCDGVTTARMAEQSLEAGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            4770..5870
                     /gene="pmrB"
                     /locus_tag="JNC24_003147"
     CDS             4770..5870
                     /gene="pmrB"
                     /locus_tag="JNC24_003147"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HAY9159834.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISIFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQYVANQI"
     gene            complement(5846..5935)
                     /gene="pmrR"
                     /locus_tag="JNC24_003148"
     CDS             complement(5846..5935)
                     /gene="pmrR"
                     /locus_tag="JNC24_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003826741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HAY9159835.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATY"
     gene            complement(6047..7549)
                     /gene="proP"
                     /locus_tag="JNC24_003149"
     CDS             complement(6047..7549)
                     /gene="proP"
                     /locus_tag="JNC24_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HAY9159836.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKYWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFTGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDDIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(7813..8679)
                     /locus_tag="JNC24_003150"
     CDS             complement(7813..8679)
                     /locus_tag="JNC24_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9159837.1"
                     /translation="MTKTLLDGPGRVLESVYPRFLVDLAQGDDARLPQAHQQQFRERL
                     MQELLSRVQLQTWTNGGMLNAPLSLRLTMVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGDQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVVLLLGDLREKATQHLAESINAAPTTR
                     HYYHQWFASSGGEHADFLSWLGKWSTADKQPVCWSVTQRWQTVALGMPRLCSAQRLVG
                     AMVEEIFSVNLA"
     gene            complement(8676..10904)
                     /gene="crfC"
                     /locus_tag="JNC24_003151"
     CDS             complement(8676..10904)
                     /gene="crfC"
                     /locus_tag="JNC24_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HAY9159838.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELSKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQQLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARHELANNGKLPAPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDALNAQLAALFQD
                     DSEPLSEIRTCCETLLQTAQNTISRDFTLRFAELESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGVHFPVFHGVVPHVNTRQLFSEVISRQDATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFCAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHGWIYTDAQLLRDDIQTLFTAE
                     RY"
BASE COUNT         2756 a         2875 c         2692 g         2691 t
ORIGIN      
        1 tattagtaaa actcgttatt tttatgcatg tttatattca tcatacaatt atataaccat
       61 ttcccggtat cgctttgctt tagcgagaac tggtctttta ttcgcactca ggagtacatg
      121 tatgaggatt tgcagcgacc aaccttgtat tgttttattg actgaaaaag atgtctggat
      181 aagggtgaat gggaaagaac ctattagcct taaagctaac catatggcgt tattaaattg
      241 tgaaaataat attatcgacg tctcctctct taacaacact ttggttgctc atattagtca
      301 cgacatcatc aaagattacc tccggtttct gaataaagat ctctcgcaaa taccagtatg
      361 gcaacgtagc gctacgccca tactcaccct gccatgcctg acgccagacg tctttcgcgt
      421 tgccgcgcaa cacagcatga tgcccgcaga aactgagtca gaaaaggaac gaacacgcgc
      481 attattattc acggtgctat cccgttttct cgacagtaaa aaattccttt cattaatgat
      541 gtatatgtta cgtaattgtg taagtgacag cgtttatcaa attattgaaa gcgatattca
      601 caaagactgg aatcttagta tggtagccag ttgtttatgt cttagcccaa gtctgttaaa
      661 gaaaaagctg aaaagcgaaa acaccagtta tagccaaata atcaccacct gccgcatgcg
      721 ttatgccgta aatgaattaa tgatggacgg taaaaatatc tctcaggtat cacagtcctg
      781 cggctacaac agtacgtcgt actttatttc tgtctttaaa gacttctacg gtatgacgcc
      841 gctgcattat gttagtcagc acagagaacg aactgtcgcc tgatttttaa ccttaacgaa
      901 gagctatatt aataacggca tcagcgataa cccggtcgat aataattcaa ctatcgaatg
      961 caggcgtatg atatgacgta atttattgtc acgaagctcg ccttcgcagg agtttaatta
     1021 tgtcttcgga tgctgatgct cacaaagtgg gcttaatccc cgtcaccctg atggtgtcgg
     1081 ggaatattat ggggtcaggt gtttttctgt tacctgcaaa cctggcctcc actggcggga
     1141 ttgccattta tggatggttg gtgacgatta tcggtgcgct ggggctctcg atggtatacg
     1201 ccaaaatgtc gttcctcgat ccaagtcctg gtggttctta cgcttacgcc cgccgctgct
     1261 ttggcccgtt tctcggttat caaaccaacg tcctctactg gctggcctgc tggatcggca
     1321 atatcgctat ggtggtcatt ggcgtaggat atttaagtta cttcttcccg attctgaaag
     1381 atccattggt attaaccatc acctgcgtcg tggtgctgtg gatcttcgtc ctgctgaaca
     1441 ttgtcggtcc gaaaatgatc acccgtgtgc aggccgttgc caccgtgctg gcgctgatcc
     1501 ccatcgtcgg gattgccgta tttggctggt tctggttccg tggtgaaacc tatatggcgg
     1561 catggaacgt cagcggcctg ggcaccttcg gtgcgattca aagtaccctt aacgttacgc
     1621 tgtggtcgtt catcggtgtg gaaagtgcct ccgttgccgc aggtgtggtg aaaaacccga
     1681 aacgcaatgt ccctatcgcc accattggtg gggtattgat tgccgccgtt tgctatgtgc
     1741 tctccaccac cgcgattatg gggatgatcc ctaatgccgc actgcgcgtt tctgcctcgc
     1801 cattcggtga tgccgcacgg atggcgttgg gtgacaccgc tggggccatt gtttccttct
     1861 gcgcagctgc gggttgctta ggttcactgg gcggctggac gttgctggcg ggtcaaacgg
     1921 cgaaagccgc tgccgatgac ggactgttcc caccgatttt tgcccgtgta aataaagcgg
     1981 gtacgccagt ggcggggctg attatcgtcg gtattttgat gaccatcttc cagctcagta
     2041 gcatttcgcc aaacgcaacc aaagagttcg gtctggtatc ttccgtgtcg gtcatcttta
     2101 cactggtgcc atatctttac acctgtgcgg cgttactgct gctcggccac ggtcactttg
     2161 gtaaagcacg cccggcatat ctggctgtta ctaccattgc cttcctctac tgcatctggg
     2221 ccgtggtggg gtccggggcg aaagaggtta tgtggtcatt tgtcaccctg atggtcatca
     2281 ccgccatgta tgcgctgaat tacaaccggc tacataaaaa cccgtatccc ttagatgcac
     2341 caataagcaa agattaattc cccttaatcc agcaaacata aaagccaacc ttaagaactt
     2401 aaggttggct taattttgct ttgcgagcat atgcgcactt tgttcgatgg aaacaccgtg
     2461 atgttgaagc gcctactaaa aagcccctct ttgaatttac tcgcctggct attgttggcc
     2521 gctttttata tctctatctg cctgaatatt gcctttttta aacaggtgtt gcaggcgctg
     2581 ccgctggatt cgctgcataa cgtactggtt ttcttgtcga tgccggtcgt cgctttcagc
     2641 gtgattaata ttgtcctgac actaagctct ttcttatggc ttaatcgacc actggcctgc
     2701 ctgtttattc tggttggcgc ggctgcacaa tatttcataa tgacttacgg catcgtcatc
     2761 gaccgctcga tgattgccaa tattattgat accactccag cagaaagtta tgcgctgatg
     2821 acaccgcaaa tgttattaac gctgggattc agcggcgtgc ttgctgcgct gattgcctgc
     2881 tggctaaaaa tcaaacctgc cacctcgcgt ctgcgcagtg ttcttttccg tggagccaat
     2941 attctggttt ctgtactgct gattttgctg gtcgccgcac tgttttataa agactacgcc
     3001 tcgttgttcc gcaataacaa agagctggtg aaatccttaa gcccctctaa cagcattgtt
     3061 gccagctggt catggtactc ccatcagcga ctggcaaatc taccgctggt gcgaattggt
     3121 gaagacgccc accgcaaccc gttaatgcag aacgaaaaac gtaaaaattt gaccatcctg
     3181 attgtcggcg aaacctcgcg ggcggagaac ttctccctca acggctaccc gcgtgaaact
     3241 aacccgcggc tggcgaaaga taacgtggtc tatttcccta ataccgcatc ttgcggcacg
     3301 gcaacggcag tttcagtacc gtgcatgttc tcggatatgc cgcgtgagca ttacaaagaa
     3361 gagctggcac agcaccagga aggcgtgctg gatatcattc agcaagcggg catcaacgtg
     3421 ctgtggaatg acaacgatgg cggctgtaaa ggtgcctgcg accgcgtgcc tcaccagaac
     3481 gtcaccgcgc tgaatctgcc tggtcagtgc atcaacggcg aatgctatga cgaagtgctg
     3541 ttccacgggc tggaagagta catcaataac ctgcagggtg atggcgtgat tgtcttacac
     3601 accatcggca gccacggtcc gacctattac aaccgctatc cgccgcagtt caggaaattt
     3661 accccaacct gcgacaccaa tgagatccag acctgtacca aagagcaact ggtgaacact
     3721 tacgacaaca cgctggttta cgtcgactat attgttgata aagcgattaa tctgctgaaa
     3781 gaacatcagg ataaatttac caccagcctg gtttatcttt ctgaccacgg tgaatcgtta
     3841 ggtgaaaatg gcatctatct gcacggtctg ccttatgcca tcgccccgga tagccaaaaa
     3901 caggtgccga tgctgctgtg gctgtcggag gattatcaaa aacggtatca ggttgaccag
     3961 aactgcctgc aaaaacaggc gcaaacgcaa cactattcac aagacaattt attctccacg
     4021 ctattgggat taactggcgt tgagacgaag tattaccagg ctgcggatga tgttctgcaa
     4081 acttgcagga gagtgagtga atgaaaattc tgattgttga agacgatacg ctgttattgc
     4141 agggactgat tctggcggcg caaaccgaag gctacacgtg cgatggcgta acaaccgcgc
     4201 ggatggcgga acaaagcctt gaggccggtc attacagcct ggtggtactg gatttagggt
     4261 tacccgacga agatggactg cattttctcg cccgtatccg gcagaaaaaa tacaccctgc
     4321 cggtactgat cctcaccgct cgcgatacgc tgaccgacaa aatcgccggg ctggatgtcg
     4381 gtgccgatga ctatctggtg aagccttttg cgctggaaga gttacacgcc cgtatccgcg
     4441 ccctgctacg acgccataat aatcagggcg aaagtgagct gattgttggc aatctgacgc
     4501 tgaatatggg tcgccgtcag gtatggatgg gcggtgaaga gttgattctg acgcccaaag
     4561 aatatgctct gctgtcacgg ttaatgctca aagcaggcag tccggtacat cgggaaattc
     4621 tctacaacga catctataac tgggacaatg aaccctcgac caacaccctg gaagtgcata
     4681 tccacaatct gcgcgacaaa gtgggcaaag cccgtatccg caccgtgcgc ggctttggct
     4741 atatgctggt cgcgaatgag gaaaactaat tgaatctgat gcgttttctg cgccgaccaa
     4801 tatcgctgcg ccaacggctg atattgacca tcggggccat tttgttggtg tttgagctga
     4861 tcagcatctt ctggctatgg catgaaagta ccgagcagat tcagctgttt gagcaggcgc
     4921 tacgcgacaa tcgcaacaac gatcgccaca tcatgcgtga gatccgcgag gcggtcgcca
     4981 gcctgattgt ccccggcgtc tttatggtca gcctgacgct atttatctgc tatcaggcgg
     5041 tacgccgcat cacccgcccg ctggcggagc tgcaaaaaga gctggaagcg cgcaccgccg
     5101 acaacttaac gcccattgcc attcacagcg ccaccctcga aatcgaagcg gtggtttcgg
     5161 cgttgaacga tctggtcagt cgcctgacga gcacgctgga taacgaaagg ttatttaccg
     5221 ctgacgtcgc gcacgaactg cgaacgccac tggcgggggt gcgtttgcat ctggaactgc
     5281 tggcgaaaac gcatcacatt gatgtagcac cgttagtggc acggcttgat cagatgatgg
     5341 agagcgtctc ccagctgctg caactggcgc gtgccggaca gtcattttct tccggtaatt
     5401 atcaacatgt aaaactgctg gaagatgtca ttctcccctc gtatgacgaa ctcagtacca
     5461 tgctcgacca gcgacagcaa accctgctat tgccagagag cgccgccgac atcaccgttc
     5521 agggtgatgc aaccctgctg cggatgttat tgcgaaacct ggtagaaaac gcccatcgtt
     5581 acagcccaca aggcagcaac attatgatta agctgcaaga agatggcgga gcggtcatgg
     5641 ccgttgaaga tgaaggaccg ggtattgatg agagtaaatg cggggagttg agtaaagcgt
     5701 ttgtacgtat ggacagccgt tatggcggga ttggtctggg gttaagtatt gtcagccgca
     5761 ttacacagtt gcatcacggg cagtttttcc tgcaaaaccg gcaagagact tccggcacgc
     5821 gggcctgggt acggctgaag aaagatcagt acgtagcaaa ccagatataa agaaagctgc
     5881 tgaccaccag cacactgaac acggtagtta aactttcata aacacggttt ttcattgcac
     5941 tctcctccgt taacctggag gagagtatgc gcgtcagaga ttaagtgaac cttaagagtt
     6001 caattggcct gataagacag cgtcacatca ggccatccgt ttcagcttat tcatcaattc
     6061 gcggatgttg ctgcaccagg cgggtacgtt tcgcctgcaa atcggcaatc tcgtggtcaa
     6121 tatcatcgat tttctgctcg atattatcgt aatgctcgac gagaatttcc ttcgcttcct
     6181 gtatatctga cgccgccggt gtcgcacctt tcaacggacg atttgccgtc tctttcatgg
     6241 ttacgccggt gattaaacca atcaccgcca ctaccatcag gtaataggca ggcatcatca
     6301 gattctgcga gctttcgacc agccaggccg ccagtgttgg cgtcagaccg gcaaccagca
     6361 ccgaaatatt aaatgccgcc gccagcgcgc tgtaacggat atgcgtcggg aacatcgctg
     6421 gcaaggtaga agccataacg cccgtaaagc agttaaggat caccgccagc atcagtaacc
     6481 cggcaaaaat caggccgatg acgttactgt taatcagaat aaacgccggg atcgccaaca
     6541 caaacagggc aacactacca agtagcacaa acggacgacg gccaaaacgg tcactcagca
     6601 agcccatcac cggctggaca aacagcatac cgatcataat ggcgataata atcagcaccc
     6661 cgtggtcttc ggagtaatgc aggttatgcg acaaataact cggcatatag gtcagcaaca
     6721 tgtagtaagt cacgttggtg gcaattacca gaccaataca tgtcaacagg ctgcgccagt
     6781 atttagtggc aatctcttta aacgagactt tcgggccatc ctgcaaacct tcgcggtcgc
     6841 cctgttccag tttatcaaca tgctgctgga acgccggagt ttcttccagc gcatggcgca
     6901 ggtaaagccc gataatccct aacggcagag caataaagaa cggaatacgc cagccccagt
     6961 cgaggaagtt cgcttcgccg acaatggtcg aaattaacac caccacgccc gcacccagca
     7021 caaacccggc aatagaaccg aagtccagcc agctgcccat aaagccacgt ttacggtcag
     7081 gggagtattc cgcaacaaat atcgacgccc cggtatattc accgccgacc gagaaacctt
     7141 gtgccatctt acagatcaac agcagaatcg gtgcccaaat accaatcgtg tcgtaggacg
     7201 gtattaagcc aatacagaac gtactgatcg acataatcac aatagtgata gcgaggatct
     7261 tctggcgacc atatttatcg cccaacatac caaagaagag cccgccaagc ggtcgaatca
     7321 gaaagggaac ggagaaagtg gcaagtgcag caaccatctg cacgctgggg tcagcccccg
     7381 ggaaaaaaac tttacctaat gcgtaagcaa caaaaccata aacaccaaaa tcgaaccatt
     7441 ccattgcatt acccagtgat gctgcggtaa tggctttacg cagtttaccg tcatcaataa
     7501 tggtgacatc acgaagggta atcggtttta cttttttcct tttcagcata gctttcctcg
     7561 cagagttggc ccatttccgc aaacgccaga aaaatcagcg cgacggccgc aaaaggggag
     7621 cgggaattgc aggatgcaat ctctgtaaca agcgtagcgg gtttgcttac accctccggt
     7681 taaggaatgt acaatcaaac cagttgacgc ctgaattggg cagttaatga cctttttacc
     7741 ctaccagtgt ttaaatttgt gatcaacttc acacatggat ttcgtttagg actcattgat
     7801 gtaactgatt atttacgcca aatttacaga gaagatttcc tcgaccattg cccccaccaa
     7861 acgctgcgct gaacagagtc gtggcatccc cagcgcgact gtttgccagc gttgggtcac
     7921 tgaccagcaa acgggctgtt tatctgccgt ggaccacttt cccagccaac ttaaaaaatc
     7981 agcatgctcc cctcccgaag aggcaaacca ctgatggtaa tagtgacgcg tggtcggtgc
     8041 cgcattgata ctctctgcca gatgttgtgt cgctttctcg cgcaaatcac ccagtaacaa
     8101 caccaccgct tgcgggcttc ccagacggca ggcatcgcca aatgcacccc agcgcaactc
     8161 ctccagaacg ataaaacagc gcccaggcaa cgaccaggcg ttatacgccc cagcacgcca
     8221 gcgggtaaaa atttgctcgc tctgatcacc tgcctgcacc agtaggcctc gttgcgttaa
     8281 cgccttttcc ttccagcgac aacgggctga aaaccactca tacaaggcgg caatttgctg
     8341 ggggagctcc gggaaagcag agtgctggcg gtgatccatt ttttgcagca gcgccagatg
     8401 ctgcgcgatc tgcgtcagcg ccagatgacc gggatccagc atcgacgcca gtttttccac
     8461 cattgtcagt cgcaggctaa gcggcgcatt taacatgccg ccgttcgtcc atgtctgaag
     8521 ctgcacacgc gaaagaagtt cctgcatcag tcgttcacga aactgctgct gatgggcttg
     8581 tggaaggcgg gcatcatcgc cctgcgccag atccactaaa aagcggggat aaaccgactc
     8641 cagcacgcga ccggggccgt ccagtaacgt cttggtcaat atcgttctgc cgtgaaaagt
     8701 gtttgaatat catcgcgtaa cagctgggcg tcggtgtaaa tccagccgtg agtcatcaca
     8761 gtctgctgca attgctgctg catcagcctg accaccgatt cattttgttg acgcagagcc
     8821 aggctttcgc gtaaacgcgt ctgtaattcc gtcaaacatg aagcgaactc agcgcaaaaa
     8881 gtattcatgc ctgccgtaac agattcatcg acctgctctg ccagaacttt agccatttgt
     8941 tggcaataaa gatcgacttc tgcgcttaaa gctcgttgca acacactgta atcaaccgtc
     9001 tctgtcgggg atttctcatt tccccgtccc cagtcgggct gattcaacca gcgcgaaaaa
     9061 gtctcacgca caacgcctaa acgcgtgctc tgctcgtccg ttgcatcctg gcgcgaaatg
     9121 acttcactga acagctggcg agtgttgacg tggggaacta ctccgtgaaa aacaggaaaa
     9181 tgaaccccag gacgaaaccc tgactcgctc aattccattt tgacttgttg ctcaatgggc
     9241 cgaataacat cggttaacac ccggcaaagg gtggattcaa gctcggcaaa acgcagcgta
     9301 aagtcgcgac tgatggtgtt ctgcgccgtc tgtaacagtg tctcacagca ggtacgaatc
     9361 tcgcttaacg gctccgaatc atcctgaaac aaggcggcta actgcgcatt cagcgcatct
     9421 tgctgttgac gcagaaagtg gttggcggag gtcagggcca gctcgatttc atgtttaatc
     9481 tcgccgctca cctgcgcctg attgagttgc aatagctgca aactttcttc gacctgatgg
     9541 atattttgcc gcaattgttc acaagcgacg tttaacccgt gcgcacgaaa atccaggtat
     9601 tcccgcgcct gctgcgcgta attcaacagt ttatgcgcag cagatcgcag agcatacaac
     9661 gaggcgttag cgtaagcggc atgaagcaac gcctgaattg gctgggcgaa cagcgaatct
     9721 tcccacaact gatcggcagc atgacgaata tgttcgaggt ccgccagatc ggcatgacgc
     9781 cagcgcctgc cgagcgcggc atgggcaaaa tcttccaccc agcgttgttg ctctggcgct
     9841 ggtaacttac cgttgttggc taactcatgg cgcgctcgat ttgccaagta gccccacatc
     9901 gacgacaccg gaaatatctg ctgtggcgta atacagcctt tcatcagcgt cccggaaatc
     9961 agtgcccgca cctggtcggc gtcgtcactg ttacgatcct gttgatcgaa cttattgacc
    10021 agcacataca gtggcaccga ttgccccacc gccaaaatcg cctcacggac ctcttcatcg
    10081 gagatcgatt tcagttgcgt ataatccagc accgccagta ccgccgaggc gcgtgccagc
    10141 tgctggttaa gcattttttg cagatgcggt tgcccggctt catttggccc gggggtatcc
    10201 agtaacgtca actgaccggg ataactctcc agccccgcca gatggacaaa ctccacttca
    10261 atcacgggaa tatgctcaat ggcggcgtaa gcagaaaaag gaaaatcgac gtccagcgcc
    10321 ttcgccagtc gcactaaatc attcaaactt ttcagacaat gaaaaatagg ctgggcaccc
    10381 agataatatt tttcgaaagc gacgccattt tcgatccgct gcataagcgc acgcatatct
    10441 ttatctattt ccagcacatc ggtcagatgc ttaatatcgc aatcacgcag gcgctgttgt
    10501 aattgttgaa ttaaacaatc gattggcgcg acatgtgaaa aatgcagtac cggttccttt
    10561 tgcccgggcg tatggcgaat aagcgtcggc agcgcagtca ttgggcgatt acgattaggc
    10621 agaacctccg taccaacaat ggcattaatg gtggttgatt tccctgcttt catagtaccg
    10681 acaatcgcca gcaccatttc cagtcgggaa attttactca actcattatt cagcatcgcg
    10741 tgacgttcgg cgatattagg ctgactccag ggtaaagcca gttgtggcgc gtcgtctccg
    10801 ggtacagaga gaggcatttt ttccagtaac tgcaactgtt ggcgagaaag ctgtaacagg
    10861 cgttcagcct cctgacttaa ctcatacagg gtctgtgtgt acatagaaaa ttcttcctta
    10921 aagcaaattt tgttactttt atttagccag attgtttttg agttctgttt gagttctgtt
    10981 tttggctttt ataattactg caagaaataa tttt
//
LOCUS       DADMKF010000131        11001 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.133, whole genome shotgun sequence.
ACCESSION   DADMKF010000131 DADMKF010000000
VERSION     DADMKF010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11001
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.133"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(<1..278)
                     /locus_tag="JNC24_003152"
     CDS             complement(<1..278)
                     /locus_tag="JNC24_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY9159839.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSR"
     gene            complement(445..1185)
                     /locus_tag="JNC24_003153"
     CDS             complement(445..1185)
                     /locus_tag="JNC24_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HAY9159840.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            complement(1278..1913)
                     /gene="argO"
                     /locus_tag="JNC24_003154"
     CDS             complement(1278..1913)
                     /gene="argO"
                     /locus_tag="JNC24_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HAY9159841.1"
                     /translation="MFSYYFQGIALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVAFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            complement(2052..2912)
                     /locus_tag="JNC24_003155"
     CDS             complement(2052..2912)
                     /locus_tag="JNC24_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HAY9159842.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            complement(3270..4349)
                     /gene="fbaA"
                     /locus_tag="JNC24_003156"
     CDS             complement(3270..4349)
                     /gene="fbaA"
                     /locus_tag="JNC24_003156"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HAY9159843.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            complement(4564..5727)
                     /gene="pgk"
                     /locus_tag="JNC24_003157"
     CDS             complement(4564..5727)
                     /gene="pgk"
                     /locus_tag="JNC24_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HAY9159844.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETASATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            complement(5777..6796)
                     /gene="epd"
                     /locus_tag="JNC24_003158"
     CDS             complement(5777..6796)
                     /gene="epd"
                     /locus_tag="JNC24_003158"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HAY9159845.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            7168..7599
                     /locus_tag="JNC24_003159"
     CDS             7168..7599
                     /locus_tag="JNC24_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAY9159846.1"
                     /translation="MMTLPYPYSSPARFYALRLLPGQEVFSQLHAFAQQHQLHAAWIA
                     GCTGSLTDVALRYAGQEGTTLLNGTFEVISLNGTLEQSGEHLHLCVSDPHGTMLGGHM
                     MPGCTVRTTLELVIGSLEELAFSRQPCALSGYDELHISPVK"
     gene            7622..8200
                     /locus_tag="JNC24_003160"
     CDS             7622..8200
                     /locus_tag="JNC24_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAY9159847.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVAGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            8201..8908
                     /locus_tag="JNC24_003161"
     CDS             8201..8908
                     /locus_tag="JNC24_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAY9159848.1"
                     /translation="MHPFTSLTLWALAACTTLILPAQTILPIYSAATFFCLIALKATR
                     RRAKYVAWLMFSLGAGLWLVHGGWLTEWLSGTPRSPERWTHAITLWLRILAIVSTSQL
                     WMQYVPVQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRARGVPLDEG
                     WYQRLRAMPALIILLTHNALNDLAVRGAALDMRAFRIHNRRTTLWAPADSTLQRVARY
                     TMILLMLTEFGAWIWLR"
     gene            8896..9573
                     /locus_tag="JNC24_003162"
     CDS             8896..9573
                     /locus_tag="JNC24_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAY9159849.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPGEITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAETEIMARIDAALTLTECQPLRHRHPATLSGGETQRVVIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWALERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            9567..10244
                     /locus_tag="JNC24_003163"
     CDS             9567..10244
                     /locus_tag="JNC24_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HAY9159850.1"
                     /translation="MLTLNQISYRWPSAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKVGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTANSHPLDLHSAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCRQRGTSVVAISHDAAFTRRHFSRVVRLEDGVIRNVN
                     PPDDIHP"
     gene            complement(10216..10929)
                     /locus_tag="JNC24_003164"
     CDS             complement(10216..10929)
                     /locus_tag="JNC24_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HAY9159851.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIERVHKPLLRMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLVQQDPELPAIQTLPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPATALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRVLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(10926..>11001)
                     /locus_tag="JNC24_003165"
     CDS             complement(10926..>11001)
                     /locus_tag="JNC24_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAY9159852.1"
                     /translation="ARFSGMVKTALTLAVTTTLKELTP"
BASE COUNT         2626 a         2920 c         2741 g         2714 t
ORIGIN      
        1 cgtgaacgac gccctgagtt atggatttca ttatcaatcg cagaaagtcg ttgataaacc
       61 gggaacagtt tttgatacag ttcctgaccg gctttattga ggctgatatt attatctttg
      121 cgggtaaata gcgtatagcc aatttgttct tccagcgcgg caatgctttt accaaatggc
      181 gaagccgtca tatggatttt ttctgccgcc cgggcaatat tattggtttg cgctaataaa
      241 atgaaattgc gcatcttttt cgaaataaaa atgtccatcc cccctccccg catccttgtc
      301 cgcttaaccg tttcagtcag cctatccttg atgaaaccgc gagcaaagat aggtgattac
      361 gtcatggttt tacagaaaat tacagaaaaa ggaggcaata tcgggtaaag gcattagccc
      421 gacgaatacg tcgggctaca agtattattg tgctgcaggt gttttagcgg gttgttgatc
      481 cacaggttct aactggaaga ccacatcgac ctgatcatca aactgaatag cggcctgctc
      541 gtaagtttcc tgggcagaca ccggcgcggc atcggctttc atcatccgca ccattgggct
      601 gggctgatag ttggaaacat ggtagcgcac gctatatacc ggccccagtt tacgatgaaa
      661 gccgttcgcc agttcctgcg cctgatgaat cgcgttatca atcgctgcct tacgcgcttt
      721 gtctttatag gcatccggct gcgccacgcc cagcgacaca gaacgaattt cgttaagacc
      781 cgccttcagc gcgccatcca gcaaggaatt cagtttgtct aactgacgga gcgtgacttc
      841 caccgttctc acagcgcggt agcctttcag gatactttta ccatcctgat aatcataatc
      901 tggctgggtg cgtaagttcg ctgagctgat atcttttttc gcgatctgat tgagttcaag
      961 gaaggaaatg tattgtgcga cgcgctcatc tgcctgtttc ttggctgtag cggcatcctt
     1021 cgcggccacg ttaacttcaa tcgcaagagt ggcaatgtct ggcaccgcat ccacacttgc
     1081 cgtaccggag gtgacaatat gcggtccatc cggcaattcg ttagcctgcg ctgccatccc
     1141 gctaataccc attaatgccg ccagggcgat aactttgaac ttcacgttag tctctccgtg
     1201 ttaattgact tgcccaaaat tcaggacctc tggaggcaag cttagcgcct ctgttttatt
     1261 tttccatcag atagcgccta actgaacaag gcttgtgcat gagcaatacc gtctctcgcc
     1321 agctgcaagg ctataaacca catgacacat cccactacca gattgataat gcgctgtgat
     1381 tttgccgtgc gcaggcgcgg tgccagccag gctgcgagaa tagccagacc aaagaaccac
     1441 aggaaagagg cgctaattgt cccgagcgca aaccaacgtt ttggttcgac gtcaagctgc
     1501 ccgccaaggc tgcccagtac aacaaaagta tccaggtaaa catgcggatt cagccaggtc
     1561 actgccagca tggtggcgat aattttccat ctgccttgct tcaggacttc ggcactagct
     1621 aactcaatat tactactcat cgctgtttta aaagcgccaa aaccatacca cagcaagaag
     1681 gctacgccgc cccaggtgac cagcgccagc aaccacggcg actgcatcaa taacgcgctg
     1741 ccaccaaaaa tcccggcgca aatcaggacc aaatcgctga tagcacaaag taaggcaatc
     1801 ataatgtggt actgacgacg aatgccctga ttcatcacaa aagcattttg tggaccgagc
     1861 ggcagaatca tagctgcccc aagagcaata ccttgaaagt aataagaaaa cacgttggat
     1921 attccgaatt aagtatctga tgcagagact atatcgcggg attgttatta gcggaaattg
     1981 ataatattaa ttggcaatca gaataactaa taagagagag gccagagagt attatctggc
     2041 ctcagcgttg attacgcagc tttgtcttct ttcacccgct taaagttcac atccatttgc
     2101 gggtacggga agctgatacc ggcggcatca aattcacgtt taatacgctc cagcacatcc
     2161 cagtacacgt tttgcagatc gccgctgttg ctccagacgc ggaccacgaa attaatcgac
     2221 gatgcaccaa gttcgttcag acgcacagtc atttcgcgat ctttcaaaat gcgatcttca
     2281 gactggataa tattggtcag gatctgctta acctgatcga tatcggaatc atacgccacg
     2341 ccaataataa attcgttacg gcgaactggc tcgcgagaga agttaataat atttccggca
     2401 ataattttac cgttcggaat aacgataatt ttaccgtctg cagtacgcat ggtggtggag
     2461 aaaatctgca cactcagcac agtaccggct acgccgccga gatcaacata ttctccggca
     2521 cggaacgggc ggaacatgac aagtaacacg ccagcggcca ggttagaaag tgacccctgc
     2581 aaagccagac caacagctaa gcctgcggca ccgagtacag caatgactga cgctgtttgt
     2641 acacccacgc gtcccagggc agcgattagc gtaaaggcga taataccgta acggactaat
     2701 gcagaaagaa aatcagcaac agtggcatcg attttacggg agatcatcag gcgattcacc
     2761 gcgttggaaa tcatccgcgc gataatcaaa ccaacgatga tgatcgcgag tgccgccacg
     2821 atgtttactg cataacttag cagcagcgcc tggttagcta ccagccagct tcccgcgccg
     2881 tttatgctat cgacaacatt caaatcttcc attcaatatt ccttttcaac tgactcaaaa
     2941 tggagaaata cactccgccg ccttatgacg ggcagtctga caagggtaaa caataaagca
     3001 taattttgcc aaatagatca cagatttctc atctgaatca ccttgctttt ggcgaaaaaa
     3061 tccccgcaga gcggggctca gacagatatc aatgtgcgct ttgtcatgcc ggatgcggcg
     3121 tgaacgcctt atccggccta caaaaacctg caaattcaat atattgcagg atacgtgtag
     3181 gcctgataag cgtagcgcat caggcaattt tgcttttggc gaaaaaaaga cccgcagagc
     3241 gggccttgag ataagcagaa aggaatatct tacagaacgt cgatcgcgtt cagttcctgg
     3301 aatgcttttt ccagacgagc gatcatcgaa gtctgaccgg cacgcagcca tacgcgcgga
     3361 tcgtagtatt tcttgttcgg ctgatcttcg cctttcgggt tacccaactg cccctgcaga
     3421 taagcttcgt tcgctttgta gtagttcaga acgccttccc aggttgccca ttgggtatcg
     3481 gtatcgatgt tcatttttac tacgccgtag cttacggagt ctttgatttc ctgagcagta
     3541 gaaccggaac caccgtggaa tacgaagttc aggctgttgt gcggcaggtt gtgtttcttg
     3601 gaaacatatt cctgagaatc acgcaggatg gtcggagtca gaaccacgtt acccggcttg
     3661 taaacaccgt gtacgttacc gaaggacgca gcgatggtga aacgcgggct gattttgctc
     3721 agttcggtgt atgcgtaatc aacgtcttcc ggctgggtgt acagtgcaga agcgtccatg
     3781 tggctgttgt ccacgccgtc ttcttcacca ccggtgcaac ccagttcgat ttccagagtc
     3841 atgccgattt tggacatgcg ctccaggtat ttagagcaga tttcgatgtt ctcttgcaga
     3901 gattcttcag acaggtcgat catgtgagaa gagaacagcg gcttaccggt agctgcgaag
     3961 tgtttttcac ccgcgtccag cagaccgtcg atccacggca gcagtttctt cgcgcagtgg
     4021 tcagtgtgca ggataaccgg aacaccataa tgttcagcca tctggtgaac gtgatgcgca
     4081 ccagagatcg cgcccaggat agcagcaccc tgcggaacgt cagatttcac gcctttacca
     4141 gcgataaagg aagcaccacc gttggagaac tgaacgataa ccggcgcttt aactttagca
     4201 gcggtttcca gtacggcgtt gatggagtca gtaccgacgc agtttactgc tggcagtgcg
     4261 aagttgtttt cttttgctac ctggaaaact ttctgtacgt catcaccagt gattacgcca
     4321 ggttttacga aatcaaaaat cttagacatg tctcttgtcc tgtatcgtcg ggccttatac
     4381 tcatcatact tcaagttgca tgtgctgcgt ctgcgttcgc tcaccccagt cacttactta
     4441 tgtaagctcc tggggattca ctctcttgtc gccttcctgc aactcgaatt atttagagta
     4501 tgaaaaaatg cgtgctctaa aagcgcgctg aaacaagggc aggtttccct gccctgtgat
     4561 tttttacttc ttagcgcgct cttcgagcat cgctactgca ggcagtactt taccttccac
     4621 gaattcgagg aatgcgccgc cgccagtgga gatgtaggag attttgtcag caatgccgaa
     4681 caggtcgatt gctgccagag tgtcgccgcc gccagcgatg gagaacgctt cgctgtctgc
     4741 gatagcgtta gccacgattt cagtaccttt gcggaagttc gggaattcga acacgccaac
     4801 cggaccgttc cacagaatgg ttttcgcatt cttcaggatt tcagccagtt cctgagcgga
     4861 agcatcaccg atatccagga tctgctcgtc agctttcaca tcgttaacag atttcagggt
     4921 agccgatgca gtttcagaga actcggttgc tacgcgaaca tcagacggaa ccgggatgtt
     4981 gcaggtggtc agcagacgtt tagcttcgtc aaccaggtca gcttcgtaca gggatttacc
     5041 cacatcgtgg ccttgtgccg cgataaaggt gttagcgata ccaccaccaa caatcagctg
     5101 gtcagcgatt ttagacaggg agtccagaac ggtcagtttg gtagatactt tagaaccacc
     5161 aacgatagcc accatcgggc gagcaggttc tttcagtgct ttacccagcg cgtccagttc
     5221 agctgccaac agcgggcctg cgcacgcaac atcagcgaat ttaccgatac cgtgagtaga
     5281 agcctgcgcg cggtgagcag taccgaatgc gtccattacg aacacgtcac acagtgcagc
     5341 gtattttttg gacagggttt cgtcgtcttt cttctcgcct ttgttgaagc gaacgttttc
     5401 cagaacaacc agttcacctt cagcaacgtc aacgccgtcg aggtaatctt taaccagacg
     5461 aaccgggtta gacagtttgt ctttcaggta gttaacaacc ggcagcagag agaattcttc
     5521 gttgtactcg ccttcggtag gacgacccag gtgggaagtt accatcactt tcgcgccttg
     5581 tttcagggcc agttcgatgg tcggcagaga agcacggata cgcgcgtcgc tggttacttt
     5641 cccgtctttt actggtacgt tcagatccgc acggataaat acacgtttcc cagcaagatc
     5701 cagatcggtc atcttaatta cagacatggt gaatcctctc gttgattcta aaagttttgc
     5761 agacgctgct tgcgtcttac ctgaaagcaa cagtagccat agctaacgtc gtgtcgagca
     5821 ttcggttagc aaagccccat tcgttatcgc accagaccaa cgttttgatc aggtgtgcgc
     5881 cactgacccg ggtttgggtg ccatcgacaa tggcactgtg cggatcgtgg ttaaaatcta
     5941 cagagaccaa cggcaattcc gtatagtcaa ctataccatg aaatgcacct tgtgctgctt
     6001 tttgcagcaa caggttgact tcattggctt ttacaggttt cttcaccgtc acgcttaaat
     6061 cgattgccgt cacatttatg gttggcacac gtaccgcaat cgcttcaaag cgatcgttaa
     6121 attgcggaaa aaatcgtgtg ataccggcgg ccagtttagt atcgaccgga atgatcgact
     6181 ggctggctgc ccgggtgcga cgcaggtcag gatggtatgc atcaataacc tgttgatcgt
     6241 gcatggcgga gtgaattgtg gtcacagtgc cggactcaat accgtacgca tcatctaaca
     6301 atttgatgac gggaattatg caattcgtgg tacaggaagc gttagaaacg atgcggtgtt
     6361 ccgcacgaag ttgatcctga ttgacgccgt aaacaacggt cgcgtcgaga tcgttactgc
     6421 caggatgtga aaagagcact tttttggccc cggcggcaat atgcgcttcg ccatgctcgc
     6481 gggagccata tacgccggtg cagtcgagga ctacatcaac gccaagttca cgccagggga
     6541 gcgattgcag tgaacgttca tgcaatacgc ggatggcgtc atcaccaaca aaaagttgat
     6601 cgcgttcctg tcgtacttcc catgcaaaac ggccatggct ggtgtcatat ttcaacaaat
     6661 gcgccatgcc cgcagcatcc gccagttcgt tgattgccac cacggtaatt tccgcccggc
     6721 gtccggattc atacaaagca cgaaccacat tacgcccgat gcgaccgaag ccatttatcg
     6781 ctacgcgtac ggtcatagat ctcctgcaag gttttccctg agcaaatttg ccagacagag
     6841 taatccagca aatcgtccgg ggaaacctta cctgtcgcaa actgcgactg attggttaat
     6901 tgtcgaacat ttaatcgact gaaacgcttc agctaggata agcgaaacgt ggaataaaag
     6961 gaatgattgt ccagtcaaag aaaacaatta tctgacttac gtcacgtttt cagccccgcc
     7021 cctgtggaat ttattccact ttgcagaatc acgaataagc gtagcaaaat cttttcgttg
     7081 tgccgtcaca aaccctgact tatatcagaa tatgactgat gcagcgtcga tattatccgc
     7141 aatacgtccg agtttcggag tactccaatg atgacattgc cctacccata ttcttccccc
     7201 gcacgctttt atgcactgcg cctcctcccc gggcaggaag ttttttccca gctacacgct
     7261 tttgcacagc agcatcaact tcatgccgcc tggattgcag gctgtaccgg tagcctgact
     7321 gacgtcgcct tgcgctatgc cgggcaagaa ggcaccacgc tccttaacgg tacattcgaa
     7381 gtgatctcac taaacggcac gctggaacag agtggtgagc atcttcatct ctgcgtttcc
     7441 gatccacacg gcacgatgct tggcggtcat atgatgcccg gctgtaccgt gcggaccacg
     7501 cttgaactgg tgatcggtag ccttgaggaa ctggcgttta gccgccaacc ttgcgcgctt
     7561 tctggttacg acgagttgca tatttccccc gttaaataac tactttcaaa gaggttttct
     7621 tatggcgcgt agccactttt ccagtcaggc actggtgttg atcgttatat ctatcgccat
     7681 caatatgatc ggcgggcaac tggccagcat ggtcaaatta cctatatttc tcgactccat
     7741 cggcacactt attagcgcgg tactgcttgg tccggtgatc ggaatgctga ccggcttact
     7801 gaccaattta ctctggggat tgctgaccga cccgatcgcc gccgcatttg cacccgtcgc
     7861 gatggtcatc ggcctggtcg ccggttggct ggcgcgagcg ggttggtttc gcaccctgcc
     7921 aaaagtcgtt gtcagcggcg tgattattac gcttgcggta acggtcgtcg ctgtaccgct
     7981 gcgtactgcg ctgttcggcg gtgtcaccgg tagcggagcc gatttgttcg ttgcctggat
     8041 gcactcgatg gggcaaaacc tggtggagtc ggtggcgatt acagtgatag gcgcgaacct
     8101 ggtcgataaa attcttacag cggtgattgt ctggctttta ctgcgccagc tgccgattcg
     8161 caccacgcgc cattttccgg caatggctgc cgtgcgctaa atgcatccat ttacctcatt
     8221 aacactgtgg gcattggcgg cctgcaccac gcttatcctg cccgcgcaga ccattttgcc
     8281 gatctatagc gcggcgacct ttttttgtct tattgcgcta aaggccacgc ggcggcgggc
     8341 aaaatatgtc gcctggctga tgttctcgct gggagccggg ctgtggctgg tacacggtgg
     8401 ctggctaaca gaatggctaa gcggcacgcc gcgttcgccg gagcgttgga cgcatgctat
     8461 tacgctctgg ttgcggattc tggcgattgt ttcaacctca cagttgtgga tgcagtacgt
     8521 ccccgtgcaa tgttttatcc gcgctctgtt tgcttctcgc ctgccgccag gcgtcgctta
     8581 tctgtttgcc ggaccgttat tggtcgttga gcagttaaaa cggcaattag cgattattca
     8641 tgaagcgcag cgtgcccgcg gcgtgccgct ggatgagggc tggtatcagc gtttacgtgc
     8701 gatgcccgcg ttaattattc tgctaacaca caatgcgctt aacgatctgg ctgtccgtgg
     8761 tgcggcgctg gatatgcggg catttcggat ccacaaccga cgaacaacgt tgtgggcacc
     8821 agccgatagc accctacaac gcgtagcgcg ttacaccatg atcttgctga tgctaacaga
     8881 attcggagcg tggatatggt tacgttagaa cagttccgtt attgcccgac acactcaacg
     8941 catcctccgt tctgttatga ctttcactat gtcaaaccgg ggatggtcgc catctttggt
     9001 gacaacggca gtggcaaaag tacgctggca cagctgatgg cgggctggta tccggacttt
     9061 ctgccaggag agataacagg cacggggacg ctcctcggta cgcccattgg gcatctcccg
     9121 cttaatgaac aatccgccac catccagctc gtccagcaat cgccctattt gcagctctcc
     9181 ggctgtacct ttagcgtgga agaagaagtt gcctttgggc cagaaaatct gtgtcttgct
     9241 gaaacagaga ttatggcgcg gattgatgcc gccctgactc tgaccgagtg ccagccgttg
     9301 cgccatcgtc acccggcaac actctccggt ggagagacac aacgcgtggt catcgcctgc
     9361 gccatcgcga tgcagccaaa gctcttaatt cttgatgaag cctttagtcg cctgacgccg
     9421 caggccagcg aaatgctgtt gcagcgctta caacactggg cgcttgaacg tggttcgctg
     9481 attattcttt ttgaacgtca tcgcacacct ttcctgaact actgccagca agcgtggcaa
     9541 ttgcaaaacg gagcgctcca gccactatgt taacgttaaa tcagatatct tatcgctggc
     9601 ctagcgcagc aacggattgt ctttgcgaca tttctctgca actcaaacaa ggtgaatggt
     9661 tggcgctgac cggcgacaat ggcgcgggaa aatcgacgct gttacgagta atggcaggtc
     9721 ttcttacgcc cacagccggt acggttatgc tgcaacaaca ggcgatgaaa aacctgaaaa
     9781 accgccagcg cgcggcaaag gttggcgtac tgtttcagga agcggaaaac caactttttc
     9841 acagcaccgt cgctgacgag atcgcatttg gtttgaaact gcaaaaatgc ccggcggatg
     9901 aaatcactca acgtacccat gctgccctgc aatgttgtca gttagcagac actgccaact
     9961 cgcacccgtt ggatctgcat agtgcgcagc gacggatggt cgccgtcgcc tgcctggaag
    10021 cactctctcc gccactatta ctgctggacg agccgagtcg ggattttgac gaaaattggt
    10081 tgagcgtgtt tgaaagctgg ctggagaagt gccgccagcg aggcacgagc gtggtagcaa
    10141 tcagtcatga cgccgcattt acccggcgtc atttctctcg tgtggtgcga ctggaagatg
    10201 gtgtaatcag gaacgttaat ccaccagacg atattcaccc gtagcggtca tcattaaggt
    10261 caaatttgca ggcagactct cttccagcac ccggcgaaca ttcggcccgt cggtacggtc
    10321 ataaaaggct tctgcatctg ccagcgatac ccctcccgcc agcttgcgcc ccaccagacg
    10381 ctcccgcaac gcagtagctg gcgcgttgat aaagatcgag aaatcacaaa attgcgcaag
    10441 ctggcaccac ttctcgtcgt ccagcaacaa ccagtttcct tcgacgatga cgagtggtgc
    10501 ggtaacgtgc aacgcatctt caacaggatc atgcttttgt cgatcgtact gcggccacgt
    10561 acaatcccct tccaccacct ggcgcagatt ttccgccagt ttcgcaacgt caaatgtctc
    10621 tggtgcgcct ttgaaggggc gcaattgatg cgcgtccagc cagctattgt aatggtgaaa
    10681 accatccatc gggagcgtct gaatagcggg cagctccggg tcttgttgca cgagatattc
    10741 ccagaaggtg gttagggtag atttacccgt ccccggcgga gcgcaaagaa aaaccaccgt
    10801 acgccgctgc ggatttaccg tctgtaaggc cgccagcata cgcagcaacg gtttatgcac
    10861 gcgttctatc tcttcatcat ggtactgcgc ttgcacgttc agcccattca ccgttaattc
    10921 aattttcacg gcgttaattc ctttaaggtg gtcgttactg ccagcgtcag cgccgttttg
    10981 accattccgc taaaacgcgc c
//
LOCUS       DADMKF010000132        10930 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.134, whole genome shotgun sequence.
ACCESSION   DADMKF010000132 DADMKF010000000
VERSION     DADMKF010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10930
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.134"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(<1..312)
                     /locus_tag="JNC24_003166"
     CDS             complement(<1..312)
                     /locus_tag="JNC24_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAY9159853.1"
                     /translation="MKAFPETFLWGGATAANQVEGAWLEDGKGSTTSDLQPHGVMGKM
                     EPRILGKENIKDVAIDFYHRYPEDIALFAEMGFTCLRISIAWARIFPQGDEAEPNEAG
                     LA"
     gene            complement(331..2208)
                     /gene="bglF"
                     /locus_tag="JNC24_003167"
     CDS             complement(331..2208)
                     /gene="bglF"
                     /locus_tag="JNC24_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HAY9159854.1"
                     /translation="MTELARKIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     NTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLGEKAQQAPENDEKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFWFFPIILG
                     YTAGKRFGGNPFTAMVIGGALVHPLILTAFENGQKADALGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPVTFLLVGPLSTWISELIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHWGLVPLCINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPSIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTVMLH
                     FITAKRQPAQGAPQEKTPEVITPPEQGGICSPMTGEIVPLIHVADTTFASGLLGKGIA
                     ILPSVGEVRSPVAGRIASLFATLHAIGIESDDGVEILIHVGIDTVKLDGKFFSAHVNV
                     GDKVNTGDRLISFDIPAIREAGFDVTTPVLISNSDDFTDVLPHGTAQISAGEPLLSII
                     R"
     gene            complement(2342..3178)
                     /gene="bglG"
                     /locus_tag="JNC24_003168"
     CDS             complement(2342..3178)
                     /gene="bglG"
                     /locus_tag="JNC24_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HAY9159855.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKCAGERINSS
                     GIEKEYALSSHELNGRLSELLSHIPLEVMATCDRIISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLPNPLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVGFIAMH
                     LVSAQMSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRILE
                     HASINDSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVRK
                     EH"
     gene            complement(3464..4189)
                     /gene="phoU"
                     /locus_tag="JNC24_003169"
     CDS             complement(3464..4189)
                     /gene="phoU"
                     /locus_tag="JNC24_003169"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HAY9159856.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDSDK"
     gene            complement(4204..4977)
                     /gene="pstB"
                     /locus_tag="JNC24_003170"
     CDS             complement(4204..4977)
                     /gene="pstB"
                     /locus_tag="JNC24_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HAY9159857.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            complement(5068..5958)
                     /gene="pstA"
                     /locus_tag="JNC24_003171"
     CDS             complement(5068..5958)
                     /gene="pstA"
                     /locus_tag="JNC24_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HAY9159858.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSVPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            complement(5958..6917)
                     /gene="pstC"
                     /locus_tag="JNC24_003172"
     CDS             complement(5958..6917)
                     /gene="pstC"
                     /locus_tag="JNC24_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HAY9159859.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            complement(7004..8044)
                     /gene="pstS"
                     /locus_tag="JNC24_003173"
     CDS             complement(7004..8044)
                     /gene="pstS"
                     /locus_tag="JNC24_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HAY9159860.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKEIGNKVNYQGIGSSGGVKQIIANTVDFGASDSPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDETIAKLNPGLKLPSQNIAVVRR
                     ADGSGTTFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTDENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            complement(8292..9365)
                     /locus_tag="JNC24_003174"
     CDS             complement(8292..9365)
                     /locus_tag="JNC24_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY9159861.1"
                     /translation="MNKYIKQWCFAVFMLSLSSVALAAPKGICTPDNGVFHRTLDFSG
                     YLITANENKVGTTFNTTVTNGSSYPGRCHCDTGNVGEFPYIYYTSKINQALTYAGVHS
                     NINYYDLNPNLDVGIAIDILGVGYVNAPFEYHANNPSGNTKYNCNRIEPLSISSGGKA
                     IVYFYIKKTFAGKLIIPETKIVTLYGTISRDTPVDYSQPMADVYIRGDITAPQSCEIN
                     NLQPVYFDFKEIPAADFSSVVGSAVTTHKITKTVTIECENLGILNTDDISTSFYATEP
                     NTDNSMVVTSNSNVGIKIYDKNNKEIKVNGGELPTDMGKSTVYGEKSGSVTFSAAPAS
                     LTGARPAPGQFTATATITVEIVR"
     gene            complement(9376..>10930)
                     /locus_tag="JNC24_003175"
     CDS             complement(9376..>10930)
                     /locus_tag="JNC24_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HAY9159862.1"
                     /translation="VTIKEEDGSEQRFTQPYASLAILKREGQTDVDVSVGELRDEDGF
                     TPDVLQAQILHGFSHGITLYGGMQAAENYGSAALGVGKDLGALGAISFDVTHARANFS
                     HDDTETGQSYRFLYSKRFDDTDTSLRLVGYRYSTEGYYTLNEWASRRNSPEDFWETGN
                     RRSCVEGTLTQSLGRDYGNLYLTLSRQQYWHTDDVERLMQFGYSSSWKRLSWNVSWSY
                     SNTARQGTGNNHASDNTSEQIYMLSLSVPLLGWWGNSYATYSVSQNDNSGSSHQLGLS
                     GTALERNNLSWNLMQSYNSHDDEVGGNMSLTYDGSYGTVNGSYNYSQNSQRLNYGIRG
                     GILAHSEGVTLSQELGETIALVKAPGAAGLEIDNMRGAATDWRGYTVKTQLNPYDENR
                     VAISDNYFSKSNIELDNTVVTMVPTRGAVVKAEFVTHVGYRVLFRVLNANGKPVPFGA
                     IAAIQDASLADSGIVGDRGELYLSGLPEKGQVTLSWGENASTKCIFNYSLSTPESESG
                     LIEQGVTCH"
BASE COUNT         2851 a         2830 c         2481 g         2768 t
ORIGIN      
        1 cgctaacccc gcttcattcg gttcggcttc gtcgccctga gggaaaattc gcgcccaggc
       61 aatggaaata cgcagacagg tgaagcccat ctcggcaaat aacgcgatat cttccgggta
      121 acggtgataa aaatcgatgg cgacatcttt gatattctct ttccccagga tgcgcggttc
      181 catttttccc ataacgccat gaggctgtaa atctgaggtc gtgctccctt tgccatcttc
      241 cagccaggca ccttccacct gattggcagc tgttgcgcca ccccaaagaa atgtttctgg
      301 aaatgctttc ataattaact ccttttatcg ttagcgaatg atggataaca gcggttcacc
      361 tgcgcttatc tgcgccgtgc cgtggggtaa tacgtctgta aaatcatcgc tattactgat
      421 taataccggc gtcgtcacat caaatccggc ctcgcgaata gcagggatat caaaagaaat
      481 cagccgatcg cctgtattga ccttgtcacc cacgttgacg tgagcggaaa agaatttgcc
      541 gtccagtttt acggtgtcga taccgacatg aatcaggatc tccacaccat catctgactc
      601 aatgccaatg gcgtgtaatg tggcgaacaa cgaagcaatt cgacccgcaa ccggagaacg
      661 cacttcacca accgagggca gaatggcgat acctttaccc aacaggccac tggcaaacgt
      721 ggtatcagcg acgtgaatga gcggcacaat ctctcccgtc atcggtgaac agataccgcc
      781 ctgctcaggt ggtgtaataa cctctggtgt tttctcttgc ggggcaccct gcgctggctg
      841 acgtttagcg gtgatgaaat gaagcatcac cgtaccgaca aatgcgcaac cgatggcaat
      901 gacaccgcca ataacgctgg cccagacggt gaaatcaatt cccgttgacg ggatggtttg
      961 catgaaggtg aaaatacttg gcaaaccaaa ggagtacact ttcgtttgcg cgtagccaat
     1021 gatggtggcc cccaaagccc cactgataca ggcgataaca aaggggtact tacgcggcag
     1081 attaacgcca tataccgctg gttcggtgat accaaacagg cccgtcaacg ccgctgatcc
     1141 cgccaccact tttttctgcg catcgcgttc gcagaggaag acgccgagcg ccgccccgac
     1201 ctgcgccata atggcgggca ttaacagcgg gatcatggtg tcgtagccca gcacggtgaa
     1261 gttattgata cacagcggca ccaggcccca gtgcagtccg aacatgacga agatttgcca
     1321 gaagccgccc attaccgcgc ccgcaaatgc aggaaccgcc tgataaagcc agagataacc
     1381 ggcggcaatc agttcgctta tccaggttga tagcggcccc accagcagaa aggtgacggg
     1441 tgtgataacc atcagacata gcaatggtgt gaagaaattt ttgattgccg acggtaacca
     1501 cgcattaagt cggcgttcca gaatgctgca caaccaggca gaaaaaataa tgggaataac
     1561 cgatgacgag taattcaaca atgtgaccgg aatacccagg aaatccagcc ccagcgcatc
     1621 cgctttttgc ccgttctcga aagcagtcag aattaatgga tgcactaacg ctccaccaat
     1681 caccatggca gtaaatggat taccgccaaa gcgtttcccc gcggtgtatc ccaggattat
     1741 cgggaagaac caaaacaagg catcactggc gctgaataaa attaaataag taccactttg
     1801 ttcggtcgtc cactgaaaag tgagcgccag agccagcata cctttcagga tcccggttgc
     1861 cgccatcaaa ccgatcagag gcgtaaaaat acctgaaata acataaacaa agcggtttag
     1921 cagattacct ttctcatcat tttccggtgc ctgttgcgct ttttcgccaa ggcctgccac
     1981 gctgttgacc gccaggaaga catcggccac atggttacct atgaccacct gaaactggcc
     2041 accgctttcc accaccataa taataccggg ggtgtttttc agtacctcag cttgcgcttt
     2101 gctttcatcc tttaacttaa aacgtaatcg cgttgcgcaa tgcatcagac taacaatgtt
     2161 atctgcgccc ccgactcctg cgactatttt tctggctaac tccgtcataa cttgccctct
     2221 accgctttgc ggcaaaactc caaaaaaaaa actgaaaaaa acggcctgac gtgaatcaag
     2281 caattttttt caggttttgc ccgcttagtg cggtaacaat cctttactca gtaataatat
     2341 ttcagtgttc tttgcgcacg cgctctatat ttatggctaa aaacataatc tctgcgggtg
     2401 aaattttacg ttgatactgc aaaccaataa aaatggcgat ccgttccgca cattgccatg
     2461 cttgcgggta attttgtttt actgcttgtt gtaatgattc atcactatcg ttaattgaag
     2521 catgttcaag aatacgccag gataaaaact tcagatgtgt aaccagtcgc tgataactca
     2581 agctttcttc ctggtaatta aggctgaact gaaattttat taattgcagc atttcgcgca
     2641 ttaactgcgt gacacctgca acatcctcca tatttccgct catttgggca ctgaccagat
     2701 gcatggcaat aaagcccact tcatctttcg gtaactgcac gcccaaccgt ttatcaataa
     2761 tggttaacgc ttcttcccct agctggaact ctttcgggta aagccgctgg atatcccaca
     2821 gcaacgggtt gggcagcaac acgttttgct gaaagcgttt aatcgcaaac tggcaatggt
     2881 cagttagcga gatataaata ctgtcctgta attttcccaa gcgctcctgc gctaaagaga
     2941 taatacgatc acaggttgcc atcacctcaa gaggaatatg acttaagagt tcgcttaatc
     3001 gcccgttcag ttcatgactg ctcaaggcat actctttttc tattccactt gagttaattc
     3061 tttcgccagc gcatttttga aagccaattc ctcgccccat gacgactttt tcccgctgtt
     3121 gatcatcaat aaccaccaca acattattgt tgagaatttt ggtgatttgc atgttcatag
     3181 caaggacctt ttttataaac aaaaaaaacc gacttcacca gtattctctg gttatgtcag
     3241 gttttgcctg cgaatgcagt aacaatccag tcatttatta atgtttttta taacgaacat
     3301 ccaggttcgg aaattaattt aattgcgtgc agagaaaata gcaatgcgct attgataaaa
     3361 atatgaccat gctcgcagtt attaactttg tgtaatttta ggaatttata aagttatata
     3421 taacaaatcc caataattaa gttattggga tttgtctggt gaattatttg tcgctatctt
     3481 ttcccgccag cagtttatcc agctcatcac cgcctacatg acggaaatcc tgccccttca
     3541 cgtagtagaa gataaactca caaatattct ggcagcggtc gccaatacgt tcgatagaac
     3601 gcgcgcagaa cagcgcagta agtacgctcg gaatggtacg cgaatcttcc atcatgtagg
     3661 tcatcagttg acgaacaata ccttcgtatt cctgatcgac ttttttatct tcacgataaa
     3721 tacgtaccgc ttcgtcaatg tccatccgcg cgaacgcgtc cagcacgtcg tgcagcatct
     3781 ggatggtatg acggcccagc gactccagac ttaccagcaa cggctgatgc tgctgggaga
     3841 atttctccag cgcagtacgg cagattttgt ccgccacgtc gccaatacgc tccagctcgg
     3901 caatggtttt actgatcacc ataaccagtc gcaggtcgct cgccgtcggc tgacgtttgg
     3961 cgataatgcg cacgcaggct tcatcgatcg ccacttccat catgttgacg ttcttgtcgc
     4021 cttcgatcac gcgcttcgcc agatcgctgt cctggttatg catcgcggtg attgcatcag
     4081 aaagctgctg ctccaccatg ccacccatgg tcatcacctg cgtgcggata ctttccagtt
     4141 cggcgttgaa ctggccggaa atatgtttat taagattgag actgtccata acgcactcct
     4201 gaattaaccg taacgaccgg tgatgtagtc ttctgtttgt ttcttcgctg gcttggtgaa
     4261 cagatcgtcc gtgttgctga actcaatcaa ttcgcccagg tacataaacg ccgtgtggtc
     4321 ggaacaacgc gcagcctgct gcatgttgtg ggtaacgatc accacggtgt aatcctgctt
     4381 cagttcggtg atcagctctt caatacgccc ggtagagatg gggtcgagcg ccgaacacgg
     4441 ttcgtcgagc agcagcactt ccgggcgaat ggcgatacca cgcgcaatac acagacgctg
     4501 ttgctgacca ccagagagag agtaaccgct ctggtgcaat ttatctttgg tttcgttcca
     4561 caatgcggct ttggtcaatg cccactgcac gcgctcgtcc atgtcggcac gggagagctt
     4621 ctcaaacaga cgaacaccaa aagcgatgtt gtcgtagatg gacatcggga acggcgtcgg
     4681 tttctggaac accatgccca ctttcgcacg cagcagtgcg atatcctgag agttggtcag
     4741 gatgttgtcg ccatcaagca gaatttcacc ttctgcgcgc tgctccggat acagttcaaa
     4801 cattttgttg aaggtacgca gcagcgtcga tttaccgcag ccggacggcc cgataaacgc
     4861 cgttacctgg tttttagcga tatccaggtt gatgtttttc agggcatgga atttgccgta
     4921 gtagaagttc aaattacgaa cctgaatttt actcggggca gtttcaacca tactcattgc
     4981 aatcttttcc tcatcgtacc tgatgctttt cgctaatcag gcttaccgta ggccgggtaa
     5041 ggcgttcacg ccgcatccgg caaagtgtta accgtgttta ttcttcgcaa aaacaacgcg
     5101 cgccagaatg ttcagcagca ggacgcacag ggtaatgatc aataccccgg cccaggccag
     5161 ttgttgccat tccgcaaacg ggctcatcgc aaacttaaag atcgtcaccg gcaggttggc
     5221 gatcggctgc atcatgtccg tgctccagaa ctggttggag agcgcggtaa acagcagcgg
     5281 cgcggtttca ccggcaatac gggcaatcgc cagcaggata ccggtcataa tcccggacac
     5341 cgacgctttc agcgtaatcg cggagatcat cttccacttc ggtgtaccca gcgcataagc
     5401 cgcttcacgc aggctgtccg gcaccagttt cagcatgttc tcggtggtgc ggataacgat
     5461 cggcacctgc aacagcgcca gcgcaattac gcccgcccag ccggagaagt gctccatctg
     5521 tgccaccacg atggtgtaga caaacagacc aaccacaatc gacggcacgg agagcagaat
     5581 gtcgttaatg aagcgaatca cttctgccag ccaggattta cgaccatatt ccgccagata
     5641 aatccccgcc ataatgccca gcggcgtacc gaataccgtg gcccacaaaa ttaacagccc
     5701 gctacccgcc agggcgttcg ccagaccacc accttccgta ttgggcggcg gcgtcatttc
     5761 agtgaacagc gccagcgaca taccgtcgat accgcgagtg atggtggaca ttaaaatcca
     5821 gatcagccag aacaggccga aggccatcgt cgccatcgag agcgtcagcg caatacggtt
     5881 tttgaggcgg cgacgcgcct gcattttgcg gcgagattca gccagcgccg cagtggtttg
     5941 catttcaacc atagccatta gcgtgccccc tcattcttag ccaggcgcat aatcataaac
     6001 ttcgatgcgg cgaggacgat gaaggtaatc acaaacagga tcaggcccag ttccatcagt
     6061 gcggcaacgt gcagaccgga ttccgcttcc gcaaattcgt tcgccaacgc agaggtgata
     6121 ctattgcccg gcatgtacag cgaggcgctg tcgagctggt aggtgttacc gatgataaag
     6181 gtcaccgcca tggtttcacc gagcgcgcgg cccagcccca gcatgatgcc gccgataaca
     6241 ccatttttgg tgaacggaag aacgatacgc cagataactt cccaggtggt gcagccaata
     6301 ccgtaggccg actctttcat catcaccggg gtttgttcga acacatcacg cattaccgcc
     6361 gcaatgtacg gaataatcat gatggcgagg atcacgcctg ccgcgaggat accgatacca
     6421 aatgcggggc cagagaacag cgcgccaaca atcgggatat tcgacatgat attgccgacc
     6481 ggctcctgaa agtaaacggc gaacagcggc gcaaagataa acaggcccca catgccgtaa
     6541 acgatacttg gaatggctgc cagcagctca atggcgatac ccagcgggcg tttcagccag
     6601 ccaggcgcaa gctcagtcag gaacagggcg ataccgaaac tcaccgggac ggcgatcagc
     6661 agcgcgataa acgaagtcac caacgtaccg tagatcggca ccagcgcccc gtagatatcg
     6721 ttcggtgcat cccactcttt ggtccatagg aaagccagac caaatttctg aatgctcggc
     6781 caggaggaga tgatcagaga gacaataatg ccacccaaca tcaatagcac aatcagcgcc
     6841 gccagtttta ccagcacgct gaaaattatg tcgccctttt tacccggtgg gttaaaagca
     6901 ggcttggttg cagccataag ttactcttca gttaaacgcg tttacaaagt cattttgtaa
     6961 tgccggatgc ggcgtaaaac accgtacccg gcctggagtt ttattagtac agcggcttac
     7021 cgctactgtc tttaatattg gtcttccacg cagcgcgaac ctgttcaact acactatccg
     7081 gcaggctggc gtaatccagg tcgttcgcct gtttagcccc ggttttgtac gcccagtcga
     7141 agaatttcag cacttctgtg ccttgttctg gtttcttctg atctttgtgg atcagaatga
     7201 acgtggtaga ggtaataggc catgcatctt cgcctttctg gttggtcaga tcctgagcga
     7261 aggttttgct ccagtctgca ccttttgctg cattagcgaa gttttcgtcg gtcggactaa
     7321 ccggtttacc atcagcggag atcagtttgg tgtacgccag gttgttctgc ttcgcgtaag
     7381 catattcaac ataaccaatt gcacccggca gacgctgaac gaacgcggcg ataccgtcgt
     7441 tacctttacc gcccagaccg atcggccatt ttacggtaga gccagtacca acgttgtttt
     7501 tccactcttc gttcactttc gccaggtagc tggtgaagac gaaggtagtc ccggagccat
     7561 ctgcgcggcg tactacagca atgttttgtg aaggcagttt cagacccgga ttcagtttgg
     7621 cgatggtttc atcatcccac ttcttgattt tgcccaggta gatgtcgccg agggttttac
     7681 catccagcac cagttcgcca gacttcagcc ctggaatgtt aaccgccagc accacgccgc
     7741 caatcacggt cgggaactgg aacagacctt cctgagccag tttttcgtca gacagcggcg
     7801 agtcagaggc accaaaatca acggtattag cgataatctg ttttacgcca ccggaagaac
     7861 cgataccctg gtagttaact ttattaccga tttctttctg gtaagtgtca gcccatttgg
     7921 catacaccgg cgcagggaag gttgcacctg cacctgtcag gcttgcttct gcaaacacag
     7981 agaaagcgct catcgataag gtcgcggcga caacagttgc gacggtggta cgcataactt
     8041 tcataatgtc tcctgggagg attcataaag cattgtttgt tggctacgag aagcaaaata
     8101 ggacaaacag gtgacagtta tatgtaagga atatgacatt tttatgacag aaaggaatta
     8161 acaaataact tactgatttt aaatgtgaag atgaaaatcc cacatgtcat ctttttgtca
     8221 tgatgcattt aaaagtgata aagaaatggg tgagatgaca acgatgtgat gaacaatcgt
     8281 tgtcagacct attagcgcac aatctcaaca gttatcgtcg ctgttgcagt aaattgcccc
     8341 ggggctgggc gagctccagt aaggctcgca ggagcagctg aaaaagttac actacctgat
     8401 ttttcaccat acactgttga tttacccatg tctgttggca actcaccacc gttcactttg
     8461 atttccttat tatttttgtc gtaaattttt atccccacgt tagagtttga agttacgacc
     8521 attgaattgt ccgtattggg ttcggtagca taaaaagacg tactgatatc atcggtattc
     8581 agtatcccca ggttttcaca ctcaatagtg actgttttag taattttatg tgttgttacc
     8641 gcacttccaa caacagatga aaaatctgca gctggtattt ctttaaaatc aaaataaact
     8701 ggctgtaaat tatttatttc gcaactttgc ggagctgtaa tatcgccccg aatataaaca
     8761 tcagccatcg gctgtgagta atccaccggg gtgtcacggc taattgttcc atacaatgtc
     8821 actatctttg tttcaggaat aatcaatttt cctgcaaatg tcttcttgat ataaaaatat
     8881 actatcgctt tacccccact ggatatactt aaaggttcaa tgcggttgca attgtacttt
     8941 gtattaccag acgggttgtt agcatgatat tcaaaaggtg cattcacata cccaacccca
     9001 agaatatcta tagcaatccc aacatcaagg ttcggattaa gatcataata attaatatta
     9061 gagtgaaccc cagcataagt taatgcctgg tttatttttg acgtataata gatatagggg
     9121 aattctccta cattaccagt atcacaatga cagcgtccag gatatgagga tccattagtt
     9181 acagtggtat taaatgtcgt tccaacctta ttttcgttcg cggtaataag atatcccgag
     9241 aaatcaagcg tacggtgaaa tacgccatta tcaggggtac agataccttt aggagctgca
     9301 agtgctacac tacttaacga gagcataaac acagcaaagc accactgttt gatatacttg
     9361 ttcataaaaa tccttttaat gacatgtcac accctgttca attaatccgc tctcactttc
     9421 tggtgtcgaa agtgaataat tgaagatgca ttttgttgag gcgttttctc cccaggataa
     9481 cgtaacctgt cctttttctg gtagaccaga aagataaagt tcgccacggt caccgacaat
     9541 tcctgaatct gccaaacttg catcttgtat cgcagcaatg gctccaaaag gtaccggttt
     9601 accatttgca tttaacactc tgaagagaac gcgataaccc acatgagtca caaactccgc
     9661 tttaaccact gcaccacgcg tgggaaccat cgtaacgacg gtattatcaa gttctatatt
     9721 cgacttcgag aaatagttat cgctgattgc tacccgattt tcatcataag ggtttagctg
     9781 tgtcttgacc gtatagccgc gccagtccgt cgcagcaccg cgcatattat ctatttctaa
     9841 cccggcggcc ccaggtgctt taacaagagc aatagtttca cctaactcct gacttaacgt
     9901 taccccttcg ctgtgtgcca gaattccccc tctgatacca taattcagcc tctgggaatt
     9961 ttggctgtag ttatagctgc cgttcaccgt gccataagag ccatcatagg tcagggacat
    10021 attaccgcca acctcatcat catgactgtt ataggactgc attaaattcc atgaaaggtt
    10081 atttctttcc agcgccgtac cgctgagtcc gagttgatgt gagctaccgg aattatcgtt
    10141 ttgcgaaaca gaataggtgg cgtaactatt accccaccag cccaataaag gaacagataa
    10201 agagagcatg tagatctgct cactggtgtt atcactggca tggttgttcc ccgtcccctg
    10261 tctggcagta ttggaataac tccaggagac gttccacgag agacgcttcc agctactgct
    10321 gtagccaaat tgcattaatc gttcgacatc atcggtatgc cagtattgtt gccggcttaa
    10381 tgtcaggtat aaattgccat aatctctccc caacgactgc gttagcgtcc cctccacgca
    10441 actacgtcgg ttacctgttt cccaaaagtc ttcagggctg ttgcgccgcg atgcccactc
    10501 attgagggta tagtagccct cggtggagta acgatagcca accaagcgca agctagtgtc
    10561 tgtgtcgtca aatcgttttg aatagagaaa gcgatatgac tgacccgttt ctgtatcatc
    10621 atggctaaaa ttcgcacgag catgtgtcac atcgaaagaa attgcgccca aagcgccaag
    10681 atctttaccg acacccagag ctgcagaacc ataattttca gcagcctgca tacctccata
    10741 taaagtgatc ccgtgggaaa aaccatgaag tatttgcgcc tgaaggacgt ccggtgtaaa
    10801 tccgtcttca tcgcgcaatt cacccacgct gacatcaaca tctgtctgac cttcacgttt
    10861 aagaatcgct aatgaagcat aaggttgggt gaatcgttgt tcgctaccgt cttcttcttt
    10921 gatggttacg
//
LOCUS       DADMKF010000133        10768 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.135, whole genome shotgun sequence.
ACCESSION   DADMKF010000133 DADMKF010000000
VERSION     DADMKF010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10768
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.135"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            92..1132
                     /locus_tag="JNC24_003176"
     CDS             92..1132
                     /locus_tag="JNC24_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HAY9159863.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLHTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(1164..2357)
                     /gene="lplT"
                     /locus_tag="JNC24_003177"
     CDS             complement(1164..2357)
                     /gene="lplT"
                     /locus_tag="JNC24_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HAY9159864.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILFAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASTIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAAARPGQSWNLINMTRSFLNACTSLWRNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKL
                     VTLETMSRCMPAGILIGVVVLIFSLQHELLPAYALLMLIGVMGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWI
                     WQRRH"
     gene            complement(2350..4509)
                     /gene="aas"
                     /locus_tag="JNC24_003178"
     CDS             complement(2350..4509)
                     /gene="aas"
                     /locus_tag="JNC24_003178"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HAY9159865.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVEMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPEL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            5095..6126
                     /gene="galR"
                     /locus_tag="JNC24_003179"
     CDS             5095..6126
                     /gene="galR"
                     /locus_tag="JNC24_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HAY9159866.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLVVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIAANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(6133..7395)
                     /gene="lysA"
                     /locus_tag="JNC24_003180"
     CDS             complement(6133..7395)
                     /gene="lysA"
                     /locus_tag="JNC24_003180"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HAY9159867.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFDVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDMLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            7517..8452
                     /gene="lysR"
                     /locus_tag="JNC24_003181"
     CDS             7517..8452
                     /gene="lysR"
                     /locus_tag="JNC24_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HAY9159868.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSEHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(8439..9086)
                     /locus_tag="JNC24_003182"
                     /pseudo
     CDS             complement(8439..9086)
                     /locus_tag="JNC24_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311724.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase"
     gene            complement(9217..10635)
                     /locus_tag="JNC24_003183"
     CDS             complement(9217..10635)
                     /locus_tag="JNC24_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HAY9159869.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGATFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
BASE COUNT         2746 a         2993 c         2617 g         2412 t
ORIGIN      
        1 ccggctggcc ttaagatttt tctctttccc ttttcccttc cctctgccat ttttatattc
       61 cttatgtcgt gattataaaa aggaaacggc tatgcaatat caccgtatac cccacagttc
      121 gctggaagtc agcacgctgg ggcttggcac gatgacgttt ggtgaacaga acagcgaagc
      181 cgacgcccac gcacaactcg actatgccgt cgctcagggc attaacctta tcgacgttgc
      241 cgaaatgtac ccagtacctc cgcgccccga aacgcaaggg ttaaccgaaa cctacgtcgg
      301 caactggctg gcgaaacatg gcagccgcga aaagttaatt atcgcctcca aagtgagcgg
      361 accgtcgcgc aataatgaca agggcatccg cccggatcag gcgctggatc ggaagaatat
      421 ccgcgaagcg ctgcatgaca gcctcaagcg cctacacact gattacctcg atctttatca
      481 ggtgcactgg ccgcagcgcc cgaccaactg cttcggcaaa ctcggttata gctggacgga
      541 ttctgcgcct gcggtttcgc tgctggatac gctggacgca ctggcagagt accaacgcgc
      601 gggaaaaatt cgttatatcg gcgtgtcgaa cgaaactgca tttggcgtaa tgcgctacct
      661 gcatctggcg gacaaacacg atctgccgcg tattgtcacc attcagaacc cttacagtct
      721 gttaaaccgc agttttgaag taggtctggc agaagtaagc cagtatgaag gggtcgaact
      781 gctggcctat tcgtgcctag gtttcggcac gctgaccggg aaatatctca atggtgcaaa
      841 acccgctggc gcacgtaata cgctctttag tcggttcacc cgctatagcg gtgagcaaac
      901 gcaaaaagcc gtcgcggcgt atgttgatat cgccagacgt catggcctag accctgctca
      961 gatggcgctc gcgtttgtac gccgtcaacc gtttgttgcc agcactctgc tgggcgcaac
     1021 cacgatggat cagctgaaaa ctaacatcga aagtttgcat ctggagttaa gcgaagacgt
     1081 attagctgaa attgaagcgg tgcatcaggt ttatacttat ccggcaccat aagattaacg
     1141 ccggttaaaa ccggcgttaa atattaatga cggcgctgcc agatccacag cgccgttatt
     1201 gccagcgcaa acagcgcacc gaagccaatg ccaatgggca cgaccgggat gcctaccatt
     1261 accgccagcg agtaaatgcc cagcatcaac aacatggcgc tgttttcgcc aaggttttgt
     1321 actgcaatcg cattccccgc cccgacgctt tttttacccc gctcctgtag caacgcattg
     1381 agcggaacga caaaaaagcc ccccatcacg ccaatcagca tcaacaaggc ataggctggc
     1441 agcagctcgt gttgcaggga aaaaatcagt accaccacgc caatcaaaat cccggctggc
     1501 atacagcgtg acatggtttc cagcgtaact aacttcgctg ccgcacctgc gccaaccacg
     1561 ataccaatcg ctaccatcgc gttgagatag gtgggcgtag cgttatcggt aatgcccagc
     1621 gccaccggta cccacagcac caacaggaaa cgcagcgtga cacccgctcc ccagaataaa
     1681 ctggtgccca ccagcgaaaa acgcgtttca ccattgcgcc atagcgaggt gcaggcattc
     1741 aggaaactgc gggtcatgtt gatgagattc caggactgcc ccggacgcgc cgccgccagt
     1801 ttgggaatgt agatattggc aacgaccgca ccaccgtagg ccagtgcgca tgcggccagg
     1861 gcgacgagga catgccagtc agccagcaca ccaccggcta cggaaccgag caaaatcgcc
     1921 gctatggtag aagcttccat taaaccgtta gctttcacta acttactacc cgtggttaat
     1981 tcgccgagaa taccgtattt cgccggtgaa taggctgcag caccaacacc caccagcgta
     2041 tagccgagaa acggattgat accaaagcag atactggctg cgcccagcag cttcaggccg
     2101 ttggcaaaca tcatcacccg gcctttggcg aagctatccg ccacctgccc gacaaacggc
     2161 gcaaaaagaa tgtaagcacc tacaaacacc atttgcagga tgggctggct ccactccgga
     2221 tagaactgcg ctttcagtaa cgccagagtg gcaaacagta gggcattatc gccaaacgca
     2281 gagagaaact gcgccacgat aaccgctttc atccccttcg accacaacga agtgttagtg
     2341 tgcactgact cactcatcgt gttgttccgc ttcgtctacc cagcttttca acgtgacaaa
     2401 gtcaggtttg ccgctgccaa gtaatggcat ctgtttcaga tagcgaatat cgcgcggtac
     2461 agcaagctcc ggtacgccgt gctcgcgggc atactgttgc aacttatcgc gcgtcagttc
     2521 gttatctgtg gtgaaaagca ccagtgcctc gcctttgctg gcatcgctct taatcgcagt
     2581 ggcatggact ttatctggcg aaacaccaag tgccagttgt tccaccattt ccagcgacac
     2641 catttcgcct gcaattttgg caaagcgttt tgcgcggccc tgaatctgca caaagccctg
     2701 ctcgtcaaaa cgcacaatat cgccagtgtc ataccagccg cgctccattt cgccgcgaac
     2761 attctcggcg gtgggcactt ccagtacacc tggcttctcc acccgcagat agccgttcat
     2821 tatgttcggc cctttcagtt gcaggcgtcc gccctcttcg ataccaggga ccgacaacag
     2881 gcgcgcatcc attcctggta gaatacgccc taccgtaccg ggtttcgccg ccatcggtac
     2941 gttgatagaa acgacaggcg cgcattcggt cacgccgtag ccttcaagga tgcgcaggcc
     3001 aaatttatcc tgccaaagct gtttggtact ttcttgtaat ttttctgcgc ctgccaccac
     3061 atagcgtaga cgatagaagt catacgggtt ggcgaaacgc gcgtagtgac cgaggaaagt
     3121 cgaggtgccg aacaacacgg tgcaactgcg gtcatacacc aactccggca caatacggta
     3181 atgcagcggg cttggataaa gaaacacttc tgcacctgtg agcagtggcg taaacaggcc
     3241 taccgtcagc ccaaaggagt gaaacagcgg taacgccgac ataaagcgat cgttggtggt
     3301 gaagtcggca atcgttttaa tctgctcgac gttcgccaga atgcttttat ggctatggac
     3361 gacgcctttc ggatggcctt cagaaccgga ggtaaagagg atcagcgcct cttcttccgg
     3421 ctgctgtttt acctgtgcca gacgtggcat cagcagatgc gcgaagatcc atactttgtc
     3481 ggcagtggtg acatctgctt ttaaatcttc cagatagacc cagcgcacct gagtaagttg
     3541 ctccggcaga tgccagagtt tgcctttatc gagaaactgg cgggaagtga agatggtttt
     3601 gatttcagcc gccgtaatag cactggtcag cccttttacc ccggcagtgt agttcatcat
     3661 tgcgggaatg cggcgacggg cgatggcccc aaaaatcact gccgcactga tgcctgcatt
     3721 gggcagcatt aagccgatgc gttcgccttc aacactgtat ttttcaagga tgcgtccaac
     3781 aaacagcgtt ttcgtaagca atttgcgata ggagtctggg gtaaagttga cgtcttcgac
     3841 acatttcttc ccggctccga agcggtacat tgcactcagt aaagattcgt acagcgtttc
     3901 acgcgggcgc accgccattc gcgcttccat cattatttga tgcagcattt caccagcgat
     3961 tttgcgacgg tcacgggcac gcggcgcatc cggcatctcc acctgcgttg gtggcaaaat
     4021 atgcagagta atttgcggga acaagcgacg tttaaccaga cctttcaggc ggctgaagtg
     4081 cgtaagttcc gccccttcaa tacgcacagg aataaccgtt gcaccagact tcgccgcgac
     4141 aaaacccgcg ccatcgtaga ttttcatcag cgagcctgtg gtggtgatgc gtccttcagg
     4201 gaaaatcacc actggtcggc cctgttccac cagacgtacc agatgtttaa tcgccatagg
     4261 ttgcgtcggg tcgagaggaa caaagtcgat aaatgatttc agccaacgca tataccactg
     4321 ttggcttatt gaggtgtaaa cggcaaacac tggacgcaca ggtaaaaaca gtccaagcaa
     4381 aatgccatca ataaaagaga cgtgattagg cgtaattaga acgcgctcgc ccttcagtgc
     4441 ctgggtgtca cccgtaacgc gaacgcgata caaaacacgg cacaaatttc gaaaaaagct
     4501 aaaaagcatt ccaactccct ttgctctgat tcagtaaaag cgaatggagg gagattacac
     4561 gagataaaga acgcgagcga cagtaaatta ggtgcgaaaa aaaacctgcg catccgcgca
     4621 ggttggtgca agagacaggg tacgaagagc gtaccgaata atctcaccaa tcaatacctc
     4681 tgggatcttg attgtggtct gcacgacgac tcttcgccag cgagaaaacg caaaggaatg
     4741 aagggaaatg caacgaggtg tgtaaattgt cggttactgt tacagattga tgaccggcaa
     4801 aaaaaaacct gcgcatctgc gcaggctggt gtaattcatg tgctcaaccc gaagttgact
     4861 tcacctatca atacctctgg gatcaccact ttagcaacct gaagccaaac gccaccagca
     4921 gtcaatcgca acagcatttc gcaaagtgta accaaaggtt tgcaatctct ttttttgtct
     4981 tacttatttg cgtttggctc acattcccac gatgaaaaca cgccgcccct tgaaccaacg
     5041 ggcgttttcc gtaacactga aacaatgtaa gcgtttaccc actaaggtat tttcatggcg
     5101 accataaagg atgtagcccg actggcaggc gtttcagtcg ccaccgtttc ccgcgtcatt
     5161 aataattcac ccaaagccag cgaagcgtcc cggctggctg tgcatagtgc aatggagtct
     5221 cttagctatc acccgaacgc caacgcccgt gcgctggcgc agcagaccac tgaaacggtc
     5281 ggtctggtcg ttggtgatgt ttccgatccg tttttcggtg caatggtgaa agcggtcgaa
     5341 caggtggctt atcacaccgg taatttttta ttgattggca atggttacca caacgaacaa
     5401 aaagagcgtc aggccattga gcaactgatc cgccatcgct gtgctgcgtt ggtcgtccat
     5461 gccaaaatga tcccggatgc cgatttagcc tcattaatga aacaaatgcc cggtatggtg
     5521 ctgatcaacc gtatcctgcc tggcttcgaa aaccgttgta ttgctctgga cgatcgttac
     5581 ggtgcctggc tggctacgcg tcatttaatt caacaaggtc atacccgcat tggttatctg
     5641 tgctctaacc actctatttc tgacgccgaa gatcgtctgc aagggtatta cgatgccctt
     5701 gctgaaagtg gtattgcggc caatgaccgg ctggtgacat ttggtgaacc agacgaaagc
     5761 ggcggcgaac aggcaatgac cgagcttttg ggacgaggaa gaaatttcac tgcggtagcc
     5821 tgttataacg attcaatggc ggcgggtgcg atgggcgttc tcaatgataa tggtattgat
     5881 gtaccgggtg agatttcgtt aattggcttt gatgatgtgc tggtgtcacg ctatgtgcgt
     5941 ccgcgcctga ccaccgtgcg ttacccaatc gtgacaatgg cgacccaggc tgccgaactg
     6001 gctttggcgc tggcggataa tcgccctctc ccggaaatca ctaatgtctt tagtccgacg
     6061 ctggtacgtc gtcattcagt gtcaactccg tcgctggagg caagtcatca tgcaaccagc
     6121 gactaaccgc agttaaagca attccagcgc cagtaattct tcgatggtct ggcgacggcg
     6181 aatcaaccgc gcctgaccat tatcaaacag aacttctggt aacagcggac ggctgttgta
     6241 gttggatgac attgatgcgc catatgcccc tgtatcatgc agtaccagat aatcacccgc
     6301 cttcacttcc ggcaaggcgc gggtttcaac atttccccct tcctgctggg taaagacatc
     6361 gcccgattca cataatggcc cggcgacgac ggtttccacc gttggcgcgt gttccagaga
     6421 acgaccatca gctgccaggg cactgatatg gtggtaacta ccgtacattg ccgggcgcat
     6481 cagatcgttg aacccggcat caaccagcac aaagtggcgg ctacccattt gtttgacgct
     6541 ccgcacctga gtaattaaca cgccagactg cgctaccaga aagcgacccg gttcaatttc
     6601 cagtttcaca gggtggccca aatggcgagc gatttgctca cgcgcggcat tccacagacc
     6661 ataataatgt tcggtatcaa ccgcctcttc accctgttga taaggaatag aaagcccacc
     6721 gcccgcagaa atagcctgta aatcctgacc gaattcgatg acctgacgta ccatagcacc
     6781 acacacctgt tccagatggg cataatcaac gccagaacca atgtgcatgt gaatgccgac
     6841 cagctgcaga tgatgacgtt gtatcacgtc cagtgcggcg ggcagatcgg tgtaccagat
     6901 accgtgcttg ctgttttcgc caccggtatt ggttttttgg ctatgtccgt gaccaaaccc
     6961 cgggttaacg cgcagccata cccgatgccc tggcgaaacc tggcccagtt ggtcgagcat
     7021 atcaacagaa cccgcattca ccggaatttg caattcactg acgcgttcaa gcgtcgcctg
     7081 atcgataaca tctgccgtaa aaacaatatc atcggggtgc gtttgcggat tgtaacccgc
     7141 cgccaacgca cgctctattt cgcctaacga gacggaatcc actttcacgc cctgctcacg
     7201 cattaatcgc aaaatatgaa tattggaaca ggctttctgt gcaaagcgca ccacatcaaa
     7261 ctgtttcagc gctgcaatct gccgacgaat aatttgcgca tcgtagaccc acaccgggca
     7321 gccaaattca gcgggcaaac gcagcagatt ttcggcggtg agatcggtat cggtgctgaa
     7381 cagtgaatgt ggcataacaa actccagatg agtgcttttt tatgattacg ccacatcata
     7441 aaaagaataa aaaatatcga tttatgtcga gtctatgcaa aaatgatata gattaccgga
     7501 ttgcgagaga gcgctaatgg ccgccgttaa cttacgtcat attgaaattt ttcatgcggt
     7561 aatgaccgcc ggaagcctga ctgaggcagc acacctgcta cacacctcac agccaaccgt
     7621 cagccgcgaa ctggcgcgct ttgagaaggt gatcgggcta aaattgtttg agcgcgtacg
     7681 tggacgatta catcctaccg tgcaaggact gcgtctgttt gaagaagtgc aacgatcctg
     7741 gtacggactg gatcgcattg tcagcgccgc agaaagtctg cgcgagtttc gccagggaga
     7801 actgtctatt gcctgcctgc cggtcttttc gcaatctttt ttaccgcagc tcctgcaacc
     7861 ctttctggca cgttatcccg atgtcagctt aaatatcgtg ccccaggaat caccgctact
     7921 tgaagagtgg ctctcggccc agcgtcatga tttaggactc actgagacac tccatacgcc
     7981 tgcgggaaca gaacgtaccg aattactctc tttagatgaa gtgtgtgtgt tacctccggg
     8041 tcatccgctg gcggtaaaaa aggtattaac gccggatgat tttcagggtg agaactacat
     8101 cagcctttcc cgtactgaca gctatcgcca gttgctggat cagctattta ctgaacatca
     8161 ggttaaacga cgcatgatcg tagaaaccca cagcgccgcg tcagtctgcg caatggtacg
     8221 ggcgggggta ggtgtttcgg tggttaaccc actcaccgca ctggattatg cggcaagcgg
     8281 tttagtggta cggcggttca gtattgcggt tccgttcacc gtcagcctga tccgccccct
     8341 gcaccgcccg tcatcagcgc tggttcaggc gtttagtgag catttacaag cggggttacc
     8401 gaaactggtc acttctcttg acgcgatttt gtcgtcagct acgacagcat aaaagcgaca
     8461 gcatcctcgg catggatcgc cgcagtatca aacacaggca aaacactgcg ctcttctgga
     8521 accaataaac caatttctgt gcagccaaaa atgacaccct gcgccccctg ttctgcaagg
     8581 cgagcaatca cttgcgcata ataagcgcgt gacgcttcgg taaattgccc cagacacagt
     8641 tcttcaaaaa taatctgatt aatcttcgcc cgttcatccg cttccggaat aagacaattg
     8701 atggaaaatt attccgtcag ccgcccgcga taaaaatcct gttccatggt gtaacgcgta
     8761 cccagcagcg ccacacgagt cattccggcc ccggtaattg cacgtccggt ggcatccgcc
     8821 actttatgca tcgtattggt acataacaca ataccttctg cgcccgcccg ctgtaagcca
     8881 agcgccgcct cagccagaat gtccccggtt ttatcccatt ccccgcgacg ctggcactct
     8941 tctatttcat gaaaatcgac gctatgtagc agcacttgcg cagagtgaag cccaccaagc
     9001 cgctgtttaa tgccttcatt tatcagacga tagtaaggaa tggtggattc ccagctcatt
     9061 cctcccagca aaccaattgt tttcattcct ctctctcctg atgtgtgtta ccgcaacaag
     9121 gctgaataag gaacgagaca aacgcctcaa cggccaggtg cccaatctct attaacgaaa
     9181 aaagggccgg atgtacagca catccggccc gtgaaatcag acgccgatat ttctcaactt
     9241 ctcgcctgcc atcagtttgc gttcgatatg ttccagcgtg acatttttgg tttccggaat
     9301 gagccagaaa gtgatgccca caaacgcaat gttcagcgca gtgtagagcc agaacgtacc
     9361 ggcagcacca atgctgtcga gcagcgtcag gaaggtcgcg ccgataatca tattcgacac
     9421 ccagttcgtg gtggtcgaac aggtaatacc gaaatcgcgg catttcagcg gctgaatttc
     9481 agagcacagg atccacacca ctggcgcggc gctcatcgca taaccggcaa tacacatcat
     9541 cgtcatgcca acagagagcc aggacaagcc actggaagcc gtaccgttat caaactgcat
     9601 caggcaatag cccagcacca gagtgcctaa cgccatcacg ctgaaaccaa ttttcagagc
     9661 cggtttacgc cctgctttat ctaccgtaaa caccgcaata aaggtggcga acataaaggt
     9721 cagccctacg accagagtcg caatcatctg ttgttctgtg gtcgtaaagc ccgccatttt
     9781 gaagatacgc ggcgcgtagt acatgatgat gttcataccg gtaaactgct gcatcgcctg
     9841 caacaacata ccgagaaaca cagcacgacg gacgttacgg ttgatcttaa acagtgccca
     9901 accgccctgt tttaacttca ggctttcacg aatttcgttg agttcttctc gcgctttttc
     9961 cgacgtatcg cgcagcatac gcaacacttc ttccgcctca atatgacgcc ccttttccgc
    10021 cagccagcgc gggctatttg gcaggaagac caccagaata atcagcagga ctgctggtaa
    10081 agcaagaacc cccaacattg cgcgccagtt accgctataa ctgaacgctg tatcggataa
    10141 aaacgccagc acgatgccga gtgtgaccat caactggtac atactgatca tcttaccgcg
    10201 aacgttttca cttgccattt cagaaagata cagaggagcg gtgtaagacg cgatcccgac
    10261 agcaatgccc agcaccaccc gagcggcgat taacatctct acgctggtcg caaaagcgga
    10321 ccctatagaa ccgagtacaa acaggatggc ccccgccatc aggctgtatt tacgccccag
    10381 gcggaacgac agccaaccat taaacagcgc accaattgct gcgccgagca tcatgctact
    10441 aaccacccat tcctgcaaac gactggtcag cacaaagtga tcggtaatga acggcaacgc
    10501 tccggcgatt acgccgatat caagaccaaa taacaatcct gcgaccgcag cagctaccga
    10561 aacaaacata ttcatacgcc gcgtatcccg caaagaacgt ggcgttaaag cagattccgt
    10621 attgatagta accatttttt cctgccagca gagagtaaga catagtgaaa aaatacgtga
    10681 acaactcaca caggtgtcag gtcggaaaca gcataaatat ggattaaatt gctgcgacat
    10741 gtcgttatgt gatggatatt ccaatttt
//
LOCUS       DADMKF010000134        10687 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.136, whole genome shotgun sequence.
ACCESSION   DADMKF010000134 DADMKF010000000
VERSION     DADMKF010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10687
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.136"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(<1..784)
                     /locus_tag="JNC24_003184"
     CDS             complement(<1..784)
                     /locus_tag="JNC24_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9159870.1"
                     /translation="MTRIACVGITVQDRIYSLPTLPEGGGKYQANHYLEIGGGPAATA
                     AVAIAKLGVEVDFIGRVGDDSCGNTLLAELEGWGVNTAFCRRYPNARSSQSAILVDQH
                     GERIIVNYPSPDLGTDAEWLEAIDFSRYDLILADVRWHSGTEKAFSLARLAGVTTLLD
                     ADMTPQDISPLVALADHAVFSTPGLKRMTGLQSPEEGLFQATTQTAGKVYVTLGSEGS
                     LWIEDGHLCQQEAFSVNVVDTTGAGDVFHGALAVALAEKMPTK"
     gene            957..1835
                     /gene="yihU"
                     /locus_tag="JNC24_003185"
     CDS             957..1835
                     /gene="yihU"
                     /locus_tag="JNC24_003185"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001318159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HAY9159871.1"
                     /translation="MATIAFLGLGQMGSPMANNLLQKGHSLQVFDVNAQAVDALVTQG
                     ATAAQTPAEAAAGAEFIITMLPNGDIVRQVLLGEKGVCEAVSSDALLIDMSTIHPLQT
                     DALIRELQEKGISMMDAPVGRTSVNAIDGTLLILAGGTDEQIARARPILMCMGNELVE
                     AGGPGMGIRVKLINNYMSIALNALSSEAAVLCESLGLNLDVAIKVMSGTAAGKGHFTT
                     TWPGKVLKGDLSPAFMVDLALKDLRIAVDVARETGAPLNMGIAAESYYAAASQDGKGR
                     QDWSALLNQVRQQAGL"
     gene            1860..2747
                     /locus_tag="JNC24_003186"
     CDS             1860..2747
                     /locus_tag="JNC24_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008786992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HAY9159872.1"
                     /translation="MSSHFTPFTLKDISRPGGGFAMLAVDQREAMRLMFAAAGQPKPI
                     ADSVLTDFKVAATRILSPYASAVLADKQFCLEQIVEQGAVANSCGLIVAADLFIPGNG
                     IPVDSVEIDMSVDPHKAREMGAKAMKLLVLWREDEPAEERLAMVDKFVRRCRSAGLVS
                     IIEPVVRPPRRGWDFDRESTIVAAAAELGGTEADLYKAEMPLGGKGDEKTLLAACQQL
                     NDQMKMPWVILSSGVDADIFGRAVSIAMKGGASGFLAGRAVWASVVGAQDPQTMLRDV
                     SVPRLQRLAEIVDEGIAQR"
     gene            2780..3790
                     /locus_tag="JNC24_003187"
     CDS             2780..3790
                     /locus_tag="JNC24_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001494590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HAY9159873.1"
                     /translation="MDKITQVLFSDIGKVTTQYVEVPHQELKPHEVRIAPVFYGICGS
                     DLHVLKGGHPFAKPPVVPGHEIAARVTEVGSDVKNVQPGDHVVVDPIMACMECRACKA
                     GRFNLCEPPQVAGFRAPGFARSQHIVPARNCHVAPASLPLKMLAFAEPAACARHCVNR
                     MPKASLESVLVIGAGTIGLSIVQALRIMGAGKITVIEPDAAKRALALKLGAAEVWAPG
                     ELAADVRFTGAIDVVAAQATLNDACTRVYAGGTVVCMGVPSGPREIPLPMMQRFERDL
                     LNSGMYIPEDFDAVIEWLADGRFDTSELVTDLFAIEDAAAAFERAQQNDSIKVMLQFA
                     PE"
     gene            3860..5293
                     /locus_tag="JNC24_003188"
     CDS             3860..5293
                     /locus_tag="JNC24_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016157870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY9159874.1"
                     /translation="MSQITSPATYSISRPQDVIDIVNKNSAINTSIGVIFIALGGILI
                     DAYQAAMVGFGNKYIAAQFGISPGLAATVNASVLIAALIGGLLANRVINRFGQKRAFI
                     IGMGLCTIGAAAVAIAPSIWWVLVCRVIMGFGLGIDFPLATNAVAELRGSTSKKTGTS
                     VNLWQMAWYVSTTVVYLVLLPLLLSGIAEEQLWRYGIFIGAIFAVIFMILRYFFIGES
                     AMWAARVGRYQEACDILGKRYGVQARVAASGTTEAKFSEKAENKYSGGYGILFNDRYR
                     KRTILGCVVATMQAWQYNAVGVYLPLTLAGIISGGLTGALTGSAVVNALCGVTGGMIG
                     SFILQRLGTRRQSMYGFAVVTLALLSLGALATTNPWLSLGLLGSIIFFHSAGPGGLGM
                     TIATLSYPPAIRPTGVGFARAIMRTGAIAGLIFWPMLWGALKTEAFYWLAIVPFLGFL
                     TCVLINWEPLGANVDAEDAEVLAELKK"
     gene            5392..6741
                     /locus_tag="JNC24_003189"
     CDS             5392..6741
                     /locus_tag="JNC24_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HAY9159875.1"
                     /translation="MSEKLPAPREGLSGKAMRRVVMGSFAGALMEWYDFFIFGTAAGL
                     VFAPLFYPDSDPFIGLIAAFATFGVGFLTRPLGGIVFGHFGDKIGRKITLIWTLAIVG
                     CSTFLIGFIPTYQEIGIWAPLVLMVLRLIQGFGLGGEYGGAALMTIESAPESRRGFLG
                     SLPQTAASVGIMLATGIFALCNHFLTSEQFLSWGWRIPFWLSAVMLIVGLFIRLHTEE
                     TLDFQKQKTTNNKEKSVPPLIELFKKHPRNILLALGARLAESVSSNIINAFGIVYISS
                     QLALSRDIPLTGMLIASAIGIFSCPLVGWLSDRIGQKSLYLSGAGFCVLFAFPFFLLL
                     DSKSTLIIWCSMILGYNLGPTMMFAVQPTLFTRMFGTKVRYTGLSFAYQFSAILGGLS
                     PLIASSLLALGGGKPWYVALFLFAVSVLSFVCVWLIEPTDEQETASYRYIREQSHEN"
     gene            6731..7618
                     /locus_tag="JNC24_003190"
     CDS             6731..7618
                     /locus_tag="JNC24_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HAY9159876.1"
                     /translation="MKTERKNEHYLALQQAFDAPWPGPVGELVTLEKGNIHLQIYPHD
                     GARITSLKAFGSEVLRQWQPQRRAFQYGCFPMVPWAGRLGNATLNAGGQCYSLPANKP
                     PHALHGMACYSTWEIIDKTADSLTLRMPLASPWPWQGEVIQTFLLENDALVLQLEVHS
                     YADTFPASAGWHPWFAKKLTPQNTESLQVLFDADWQEEAGSDELPTGNRIFPQAGPWD
                     DCFGFYNGVKVKLLWPGKLAMTMISSANSLVVFDKQPDATCINPLTQAPNAINLTPEL
                     VTPDRPLVIETRWQFTPES"
     gene            7759..8358
                     /gene="yihX"
                     /locus_tag="JNC24_003191"
     CDS             7759..8358
                     /gene="yihX"
                     /locus_tag="JNC24_003191"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HAY9159877.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRIPLASLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            8352..9224
                     /locus_tag="JNC24_003192"
     CDS             8352..9224
                     /locus_tag="JNC24_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HAY9159878.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLFSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            9221..9658
                     /gene="dtd"
                     /locus_tag="JNC24_003193"
     CDS             9221..9658
                     /gene="dtd"
                     /locus_tag="JNC24_003193"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HAY9159879.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            9703..10644
                     /gene="fabY"
                     /locus_tag="JNC24_003194"
                     /gene_synonym="yiiD"
     CDS             9703..10644
                     /gene="fabY"
                     /locus_tag="JNC24_003194"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HAY9159880.1"
                     /translation="MYHLRVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFINQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
BASE COUNT         2462 a         2533 c         2854 g         2838 t
ORIGIN      
        1 cttttgttgg cattttttct gccaacgcta ccgcgagtgc gccgtgaaaa acatcgcctg
       61 ctccagtggt atcaaccaca ttgacagaaa atgcctcctg ttgacacaaa tgaccatctt
      121 caatccacaa cgatccttca gaacccagcg taacatacac tttacctgct gtttgtgtcg
      181 ttgcctggaa caaaccttct tcaggtgact gcaggccagt catgcgtttc agccctggtg
      241 ttgagaacac ggcgtgatcg gcaagcgcga caagtggaga aatatcctgc ggtgtcatat
      301 cggcgtctaa aagcgttgtc acacccgcaa ggcgcgctag cgagaatgct ttttcagtac
      361 cgctatgcca gcgaacatcg gccagaatca gatcataacg agagaaatcg atcgcttcta
      421 accattcggc atcagtaccg agatcaggac ttggataatt aacgataatc cgttcgccat
      481 gctgatcaac gaggatcgct gattgcgacg aacgggcgtt aggatatcgg cggcaaaatg
      541 cggtattgac gccccaaccc tcaagttctg caagtaaggt atttccacaa ctgtcatcgc
      601 cgacgcgacc gatgaaatca acctccacgc caagctttgc gattgcaaca gcggctgttg
      661 ctgccgggcc gccaccaatc tcaagataat gatttgcctg atatttcccg ccgccctccg
      721 gcagagtggg caaagaataa attctgtcct gaactgtgat acccacgcat gcaattctgg
      781 tcatatttgt cttgtcctgt ttaattttgt cgtctttggt caatttaaat gacgcaaatg
      841 atcaaattag tgatgcgtgt cgtgtttttg aacataaatt acaaatatgc tcaaaaacag
      901 acgaaaacga attttcaatt gtcattattt tgtcaatgtc agacaggaga gtattcatgg
      961 caaccattgc atttctcggg ctggggcaga tgggtagccc aatggctaac aatctattgc
     1021 aaaaagggca ttcattgcag gtatttgatg tcaatgcaca ggccgtcgat gcgctagtta
     1081 cgcagggggc cactgcggca caaaccccag cggaagcagc agcaggcgct gaatttatca
     1141 tcactatgct tcccaatggt gacattgttc gacaggttct gttgggggaa aaaggcgtct
     1201 gtgaggctgt ttcatctgac gcgttattga ttgatatgtc gactattcat cccttacaga
     1261 ccgatgcatt aatacgggag ttacaagaaa aaggcatcag tatgatggac gctcccgtgg
     1321 gcagaacgtc cgtgaatgcg attgatggca cattgctgat tttagcgggt ggcactgatg
     1381 agcagattgc acgtgcacgc ccgattctca tgtgcatggg taatgagctg gtcgaggctg
     1441 gcggcccagg aatggggatc cgcgtgaagc taattaacaa ctatatgagt attgcgctta
     1501 atgcgctgtc ctccgaggcc gctgtgttat gtgagtcact gggattaaat cttgatgtcg
     1561 caattaaggt gatgagtggt acggcagcag ggaaaggaca ttttacgacc acctggcctg
     1621 gcaaagtgct gaaaggcgat ctttcacccg ccttcatggt ggatctggcg ttgaaagatc
     1681 tgcgtattgc agttgacgtc gctcgggaga cgggggcacc gctcaatatg gggattgcag
     1741 cagaaagtta ttacgctgca gcaagtcaag acggcaaagg gcggcaggac tggagtgccc
     1801 tgctaaatca ggttcgccag caggctggtc tttaatttta tctattaaca ggagtttcta
     1861 tgtcatccca cttcacccca tttacgctga aagatatctc ccgcccaggt ggtggatttg
     1921 ccatgttagc tgtcgatcag cgtgaggcca tgcgcttgat gtttgcggca gcagggcagc
     1981 caaaacccat cgctgacagt gtactcaccg attttaaagt tgccgcgacc cgaatccttt
     2041 ctccttatgc ttctgccgtt ctggcggata aacaattttg tcttgagcaa atagtcgagc
     2101 agggcgctgt tgctaattct tgcggcctga ttgtcgctgc cgatcttttt atcccaggta
     2161 atggcatccc ggttgatagc gttgaaatag atatgtccgt cgacccgcat aaagcccgtg
     2221 aaatgggggc taaggcaatg aaattacttg tactctggcg tgaagatgaa cccgcagagg
     2281 aacgtctggc aatggttgat aaatttgttc gtcgctgccg tagtgccggg ctggtgagca
     2341 ttattgaacc tgtggtgcgt ccacctcgtc gcggttggga ctttgatcgt gaaagcacca
     2401 ttgttgccgc cgccgcagag cttggtggaa cagaggctga tctttacaaa gccgagatgc
     2461 cattaggggg caaaggcgat gaaaaaacct tacttgccgc ctgtcagcaa cttaacgatc
     2521 agatgaagat gccgtgggta attctttctt ctggcgttga tgccgatatt tttggccgtg
     2581 cagtaagtat tgccatgaaa ggtggtgcgt cgggcttcct ggctggacga gcggtttggg
     2641 cgtcggttgt tggtgcacaa gatccacaaa ctatgctgcg cgatgtctct gttcctcgtt
     2701 tacaacggtt ggcagaaatt gtcgatgaag gtatcgcgca acgctaatcc cctatggata
     2761 tcgattctgg agaataacaa tggataaaat tacccaggtt ttattttcag atattgggaa
     2821 agtcaccact caatatgttg aagtaccaca ccaggaactt aaaccgcacg aagtgcggat
     2881 tgcgcctgtg ttctatggga tatgcggttc ggatctgcat gttctgaaag gcggtcatcc
     2941 gtttgccaaa ccacctgtcg tccccggtca tgaaattgca gcgcgcgtta cggaagttgg
     3001 cagcgacgtt aaaaatgtac agccgggcga tcatgttgtg gtcgatccca tcatggcttg
     3061 catggaatgc cgagcctgca aagcaggacg ttttaatctt tgtgaaccac cgcaggttgc
     3121 tggttttcgc gcaccgggct ttgctcgctc acaacacatt gttcctgcgc gtaattgcca
     3181 tgtcgcacca gcctctttac cgctaaaaat gttggccttt gccgaaccgg cggcttgtgc
     3241 ccgtcactgc gttaaccgaa tgccgaaagc ttctctggaa agcgtactgg taattggtgc
     3301 cggaacgata ggcttatcca tcgtgcaggc actgcgcatt atgggggcag gtaagattac
     3361 cgtgattgaa cctgacgctg ccaaacgcgc gctggcgtta aagctgggcg cagcagaagt
     3421 ttgggcacca ggtgagctgg ccgcagatgt gcgatttacg ggggcgattg atgtcgttgc
     3481 agcgcaggcc acgcttaacg atgcatgtac ccgtgtatat gccggaggca ccgtcgtgtg
     3541 catgggcgta ccaagtgggc cgcgtgaaat accattaccg atgatgcaac gtttcgagcg
     3601 tgacttgctc aactctggca tgtacatccc tgaagatttc gatgctgtta tcgaatggct
     3661 ggcggatggg cggtttgata ccagtgaact ggttaccgat ttatttgcca ttgaggatgc
     3721 agcggcggca tttgaacgcg cgcagcaaaa tgactccata aaggtcatgc tgcaatttgc
     3781 gccggaatga ttttctaaat ctctgtctgg cgctgcgtca cgtcagcgcc accctacaac
     3841 aattaaaaag gacactgcga tgagtcaaat caccagtcct gcaacttatt caattagtcg
     3901 gccacaggac gtaattgata ttgttaataa gaactctgca atcaacacca gtatcggcgt
     3961 tatttttatt gctttgggcg gcatattgat tgatgcctac caggcggcga tggtggggtt
     4021 tggtaataaa tacattgccg ctcaattcgg catttcgccg ggtcttgctg caaccgtcaa
     4081 tgcatcagta ttaatcgccg cgttaattgg cggtttatta gcgaaccgag taataaaccg
     4141 ctttgggcaa aagcgggcat ttattattgg catggggctg tgcaccatcg gtgctgcagc
     4201 ggtagctatt gctcccagta tctggtgggt gctggtgtgc cgcgtcatca tgggctttgg
     4261 tttaggcatc gacttccctt tggcaaccaa tgccgtggca gagcttcgtg gttcaacgtc
     4321 gaagaaaacc ggaacgtcgg tcaacctctg gcaaatggcc tggtatgttt cgacaaccgt
     4381 tgtttatttg gtacttttgc cgctgcttct gtcgggtatc gctgaagaac aattgtggcg
     4441 ttacggaata ttcatcggag ctatttttgc agtcatcttc atgattttgc gttacttctt
     4501 tattggtgaa tccgcaatgt gggccgcacg cgtcgggcgt taccaggaag cgtgcgacat
     4561 tctgggaaaa cgttatggtg ttcaggctcg cgttgcggca tcgggtacaa cagaagcgaa
     4621 attctcggaa aaagcagaga ataaatacag tggtggatat ggcatcttat ttaatgatcg
     4681 ttaccgcaaa cgcaccattc ttggctgtgt cgtggcaacc atgcaggcgt ggcaatataa
     4741 cgccgtaggt gtttatcttc ctcttacgtt ggcgggaata ataagtggcg ggcttactgg
     4801 cgcgttgacc ggttctgccg tcgtgaatgc cctttgtggg gtgacaggcg ggatgatcgg
     4861 ctcgtttatt ctccaacgac tgggtactcg ccgacagtcg atgtatggat ttgctgttgt
     4921 gaccttagca ttgctgtcgt taggcgcact ggcaacgact aatccctggc tgtctttagg
     4981 gttattggga tcaattattt tcttccattc agcgggtcct ggtgggctgg gcatgaccat
     5041 tgccacactc tcttatcctc ccgctattcg ccctactggg gtcggatttg cccgcgctat
     5101 tatgcgcaca ggggcaattg caggactcat tttctggccg atgctgtggg gtgcgttgaa
     5161 aactgaagcg ttttactggt tggcaatcgt gccattcctg gggttcctga cctgcgtatt
     5221 gattaattgg gaaccactgg gtgcaaatgt tgatgctgag gatgcagagg ttctggctga
     5281 attgaagaaa taaattaatg cgcatcggct taaaccgatg cgcattaatt caaactgccc
     5341 acgtttagtg gttcgtatcc cagatttctc gcctaccagg agaggaaaat tatgtctgaa
     5401 aagttacccg caccgcgtga agggctttcc ggtaaagcca tgagacgtgt cgttatgggc
     5461 agtttcgcag gcgcgttaat ggaatggtat gacttcttta tttttggcac ggcagcagga
     5521 ctggtatttg caccgctgtt ttatccagac agcgatcctt ttataggtct tatagccgcc
     5581 tttgcgacct ttggtgtcgg ttttctgact cgtcctttag gtggcatcgt ctttggtcat
     5641 tttggcgata aaatcgggcg aaaaataaca cttatctgga cgctggctat tgtcggttgc
     5701 tccacatttt tgattggttt tattccgacg tatcaggaaa ttggcatttg ggcaccgctt
     5761 gtattaatgg tgctgcgttt gattcagggc tttggtcttg gtggcgagta cggcggagcg
     5821 gcattgatga ccatagaatc agcgcccgaa tcccgacgcg gatttttagg ttcactcccg
     5881 caaacggctg cgtctgtggg tattatgctg gcaaccggta tttttgctct ctgtaaccat
     5941 tttcttactt cggagcagtt tctctcctgg ggatggcgca ttccgttttg gctttctgcg
     6001 gtgatgttga tcgttgggct gtttatccgc ctgcataccg aagaaacact ggattttcaa
     6061 aagcaaaaaa cgacgaataa taaagaaaag tccgttcctc cgttgattga attattcaaa
     6121 aaacatccac gaaatatttt attggcactg ggtgcgaggc tggcggaaag tgtctcctct
     6181 aatattatta acgcctttgg tattgtctat atttccagcc aattagcatt gtcgcgagat
     6241 attcccctga cgggtatgtt gattgcctcg gcgatcggta ttttcagttg tccattggtt
     6301 ggatggttat cagatcgtat aggtcaaaaa agtctgtatt tgtcgggagc aggattttgt
     6361 gtcctgttcg cgtttccttt cttcttatta ctggatagca aaagtacgct catcatctgg
     6421 tgcagtatga tcctcggcta caacttaggt ccgacgatga tgtttgccgt gcagccaaca
     6481 cttttcaccc gcatgttcgg taccaaagta cgatataccg gcctttcttt tgcttatcaa
     6541 ttttccgcga ttcttggcgg actaagcccc cttatcgcct caagtttgct ggcgctaggg
     6601 ggaggaaaac cgtggtatgt cgctctgttt ttatttgctg tctcagttct ttcctttgtt
     6661 tgcgtctggc tgattgagcc gaccgatgaa caagaaaccg cttcttaccg ctacatcagg
     6721 gaacaatctc atgaaaactg aacgaaaaaa cgagcattac ctggcgctac aacaggcgtt
     6781 tgacgcgcca tggcctgggc ctgtaggaga gttagtgacg ctggaaaaag gcaatattca
     6841 cctgcagatt tatccgcatg atggtgccag gataacctcg ttaaaggcat ttggttctga
     6901 agtattacgc caatggcagc cgcagcgcag agcttttcag tatggctgtt tcccaatggt
     6961 gccgtgggca gggcgactgg ggaatgcgac gctgaatgct ggtgggcaat gttattcttt
     7021 acccgccaat aaaccaccac atgcgttgca tggtatggct tgttactcaa cgtgggaaat
     7081 catcgacaaa acagcagatt ccctgacttt acgtatgccc ctggcttcac cgtggccctg
     7141 gcaaggggaa gtgattcaga cttttttgct ggagaacgat gcgctggttt tgcaactgga
     7201 agtccattca tatgccgata cattcccggc ctccgcaggc tggcatccgt ggtttgcgaa
     7261 aaagctgact ccgcaaaata cagaatcgtt gcaggtgctt tttgatgcgg actggcaaga
     7321 agaagcgggt agtgatgaac tgccaactgg aaatcgtatc tttcctcaag ccggtccctg
     7381 ggatgactgt tttggttttt ataacggggt gaaggttaag ttactgtggc ctggtaaact
     7441 cgcgatgacc atgatctctt ctgcaaacag tctggtggta tttgataaac agcccgatgc
     7501 cacctgtatt aacccgctga ctcaggcacc taacgcaata aatctaacgc cggaattggt
     7561 gacaccagat aggccgctgg tcatcgaaac tcgttggcaa tttacacctg aatcttaacc
     7621 attcatctat tgcgaaaaag ccagtgaaat tactctgctt tttcgcaatc agcatcacaa
     7681 gattaccctt attgccacat agccaaaccc atcctttccc gctacagtta atttcttgtg
     7741 gcgcgaaagg aggcaaaaat gctctatatc tttgatttag gtaatgtgat tgtcgatatc
     7801 gactttaacc gtgtgctggg agcctggagc gatttaacgc gtattccgct ggcatcgctt
     7861 aagaagagtt ttcatatggg ggaggcgttt catcagcatg agcgtgggga aattagcgac
     7921 gaagcgttcg cagaggcgct gtgtcatgag atggctctac cgctaagcta cgagcagttc
     7981 tctcacggct ggcaggcggt gtttgttgcg ctgcgcccgg aagtgatcgc catcatgcat
     8041 aaactgcgtg agcaggggca tcgcgtggtg gtgctttcca ataccaaccg cctgcatacc
     8101 accttctggc cggaagaata cccggaaatt cgtgatgctg ctgaccatat ctatctgtcg
     8161 caagatctgg ggatgcgcaa acctgaagca cgaatttacc agcatgtttt gcaggcggaa
     8221 ggtttttcac ccagcgatac ggtctttttc gacgataacg ccgataatat agaaggagcc
     8281 aatcagctgg gcattaccag cattctggtg aaagataaaa ccaccatccc ggactatttc
     8341 gcgaaggtgt tatgctaaaa accattcagg acaaagccag gcaccgtacc cgtccactat
     8401 gggcctggct aaaactactc tggcaacgta ttgatgagga caacatgaca accctggcag
     8461 gtaaccttgc ctatgtgtct ttgctctcat tagtgccgct ggttgccgtt gtttttgcgc
     8521 ttttcgccgc ttttcccatg ttttccgacg tcagcattca gttacgtcac tttatttttg
     8581 ccaactttct gcctgctact ggcgatgtta ttcagcggta tatcgaacaa tttgttgcca
     8641 attccaacaa gatgaccgcc gttggggcgt gcgggctgat cgtcaccgcg ttattgttga
     8701 tgtactccat cgatagcgcg ttgaatacca tctggcgcag taaacgagcg cgaccaaaaa
     8761 tttactcgtt tgccgtgtac tggatgatct taacgctggg gccgctgctg gcaggggcca
     8821 gtctggcgat cagttcctat ttgttctccc tgcgctgggc gagcgatctc aatactgtca
     8881 tcgataacgt gctgcgtatt tttccgctgc tgttgtcgtg gatctccttc tggttgctgt
     8941 acagcattgt tcctaccatc cgcgtaccta accgcgacgc gattgtcggc gcgtttgtcg
     9001 ctgcactcct gttcgaagca ggaaagaaag gtttcgcgct ttatatcacc atgttcccgt
     9061 catatcagct catttacggc gtgctggcgg tgatccccat tctcttcgtt tgggtctact
     9121 ggacatggtg tatcgtcttg cttggcgcgg aaattactgt cactctcggg gaataccgca
     9181 aactaaaaca agcagctgaa caagaagaag acgacgaacc atgattgcat taattcaacg
     9241 cgtaacccgt gccagcgtca ccgtggaggg agaagtgacg ggcgaaattg gcgcgggact
     9301 tttggtgtta ttgggtgtcg aaaaggatga cgacgaacag aaagcaaacc gtctgtgcga
     9361 gcgtgtgctc ggctaccgca tctttagcga tgccgaaggc aagatgaatc tcaacgtgca
     9421 acaggcgggc ggcagtgtac tggtggtttc ccagtttacc ctcgccgcag ataccgaacg
     9481 ggggatgcgc ccaagtttct ccaaaggcgc atcaccggat cgcgcagagg cgttatatga
     9541 ctatttcgtc gaacgctgcc gccagcaaga gatgaacacg caaacaggac gcttcgctgc
     9601 cgatatgcag gtatcgctgg tcaatgatgg ccccgtgaca ttctggttgc aggtatgagc
     9661 ctgcttccag ggttgtcacg ggaaacaaga gagagtatcg ctatgtatca ccttcgggtt
     9721 ccacaaacag aagaagagtt agagcgttac tatcagtttc gctgggaaat gttgcgtaag
     9781 cccctgcatc aaccaaaagg ttcggaacgc gacgcgtggg atgcgatggc gcatcaccag
     9841 atggtcgtcg acgagcaggg taatctggtg gcggtaggcc gattgtatat taatgccgac
     9901 aatgaagcgt ccattcgctt tatggccgtt cacccggacg tgcaggacaa agggttagga
     9961 acgctgatgg cgatgaccct ggagtcggtg gcgcgtcagg aaggcgttaa gcgcgtgacc
    10021 tgtagcgccc gtgaagacgc ggtggagttt ttcgccaagc tgggattcat taatcagggg
    10081 gagatcacca ccccaactac cacgccgatt cgccattttt tgatgattaa acccgttgcc
    10141 actctggatg acattctgca tcgcggtgac tggtgcgcgc agctgcaaca ggcgtggtac
    10201 gaacatatcc cgttaagcga aaaaatgggc gtgcgcattc agcaatatac cgggcaaaaa
    10261 tttatcacca ccatgccaga aaccggtaat cagaatccgc accatacgtt gtttgccggg
    10321 agtttattct cgctggcgac gctcaccggt tggggactta tctggctgat gctgcgtgaa
    10381 cgccacctcg gcggaacgat tattctggcg gatgcgcata tccgctacag caagccgatt
    10441 agcggtaaac ctcatgcggt agccgacctt ggtgccttaa gcggcgatct cgaccgtctg
    10501 gcgcgcggac gaaaagcacg ggtgcagatg caagttgaaa tctttggcga cgagacgccg
    10561 ggtgcagtgt ttgaaggcac gtatatcgtt ctgcccgcga agccatttgg cccgtatgaa
    10621 gagggcggga acgaagaaga gtagcttatg gagaggggcg gtgctgcctc tctcattcat
    10681 tccggtc
//
LOCUS       DADMKF010000135        10647 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.137, whole genome shotgun sequence.
ACCESSION   DADMKF010000135 DADMKF010000000
VERSION     DADMKF010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10647
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.137"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            319..894
                     /gene="ssuE"
                     /locus_tag="JNC24_003195"
     CDS             319..894
                     /gene="ssuE"
                     /locus_tag="JNC24_003195"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HAY9159881.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFAP
                     EDLLYARFDSPALKTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSAMKAQELLHGVFADDSQVIDYHHKPQFTPNLQTR
                     LDTALETFWQALHRRDVQVPDLLSLRGNVHA"
     gene            887..1846
                     /locus_tag="JNC24_003196"
     CDS             887..1846
                     /locus_tag="JNC24_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY9159882.1"
                     /translation="MRNIIKLALAGLLSVSTFAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLIYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VSAEVAALQQQTADLFYENRLVSKKVDIRQRIWQPTHLEGKQL"
     gene            1843..2990
                     /gene="ssuD"
                     /locus_tag="JNC24_003197"
                     /pseudo
     CDS             1843..2990
                     /gene="ssuD"
                     /locus_tag="JNC24_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309045.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
     gene            3002..3793
                     /gene="ssuC"
                     /locus_tag="JNC24_003198"
     CDS             3002..3793
                     /gene="ssuC"
                     /locus_tag="JNC24_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HAY9159883.1"
                     /translation="MATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPEG
                     VVTAFWTLSASGELWQHLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQ
                     MLRNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARS
                     YGLSGIPLFIHVILPGALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREF
                     LQTDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATV"
     gene            3790..4557
                     /gene="ssuB"
                     /locus_tag="JNC24_003199"
     CDS             3790..4557
                     /gene="ssuB"
                     /locus_tag="JNC24_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HAY9159884.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLRLLAGLETPTAGDVLAGTTPLAEIQEDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAVGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQQHGFTVLLVTHDVSEAVAMADRVLLIEEGKIGLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMRRGHSEQLIRRHG"
     gene            complement(4764..7376)
                     /gene="pepN"
                     /locus_tag="JNC24_003200"
     CDS             complement(4764..7376)
                     /gene="pepN"
                     /locus_tag="JNC24_003200"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HAY9159885.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEGALVISNLPEHFTLKIVNEISPAA
                     NTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKTKYPFLLSNGN
                     RVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDRG
                     NLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKYV
                     LARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSDL
                     GSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMIH
                     TLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPIVTV
                     KDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVNS
                     VLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRWD
                     AAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVNE
                     MAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLRN
                     ACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKWH
                     QDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAFH
                     AEDGSGYQFLEEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLEN
                     LSGDLYEKITKALA"
     gene            7642..8844
                     /gene="pncB"
                     /locus_tag="JNC24_003201"
     CDS             7642..8844
                     /gene="pncB"
                     /locus_tag="JNC24_003201"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HAY9159886.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            9016..10416
                     /gene="asnS"
                     /locus_tag="JNC24_003202"
     CDS             9016..10416
                     /gene="asnS"
                     /locus_tag="JNC24_003202"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HAY9159887.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
BASE COUNT         2432 a         2723 c         2839 g         2653 t
ORIGIN      
        1 ctttaaatga cattagactt aataataaca ataattgacc gccatttaaa tcaagcatac
       61 taaatccaat aagtgacaaa tctttaattt atagatttca tgaatattta aatattaact
      121 gaacgagtaa ttcattgatt caacatctaa tgactatcca acaaataaat atgcgtgata
      181 tataaagccc tttctttagt ttattttcag aaaaagatac acaaaattta tatttggcat
      241 tttcttgtct ctccgacaga ctgaccgaat tgttatcaat gttaacaaaa aaagaacaat
      301 tggttataag gagagagtat gcgtgtcatc accctggcgg gtagtcctcg ctttccttct
      361 cgctccagct ccttgctgga atatgcgcgg gaaaaactaa atggcctgga tgtagaggtt
      421 tatcactgga atctgcaaaa cttcgccccg gaagatctgc tttacgctcg tttcgatagt
      481 ccggcactca agaccttcac cgaacagctg caacaggctg atggactgat agtcgctaca
      541 cctgtgtata aagccgccta ttccggcgcg ctgaaaaccc tgctcgacct gctgccagag
      601 cgcgctttgc aaggcaaagt ggtgctaccg ctggcgacgg gcggtacggt ggctcatctg
      661 ctggcggtcg attatgccct taagccagtt ttaagtgcga tgaaagccca ggagctcctg
      721 cacggcgtgt ttgccgatga ctcacaagtt attgattacc atcacaaacc ccagttcacg
      781 ccaaatctgc aaacccgtct tgataccgcg ctagaaactt tctggcaggc attgcaccgc
      841 cgcgatgttc aggttcctga ccttctgtct ctgcgaggta atgtccatgc gtaacatcat
      901 taaactggcg ctggcgggat tgcttagcgt ctctactttt gcggttgctg cagaatcctc
      961 gcctgaagcg ttacgtatag gctatcagaa aggcagtatt ggtatggtat tggcaaaaag
     1021 ccaccagtta ctggaaaaac gctatccgca atcaaaaatc tcctgggtgg agttccccgc
     1081 gggtccgcaa atgttggaag cgttaaacgt tggcagtatt gatctcggca gtaccgggga
     1141 tattccgcca atctttgccc aggctgccgg ggctgatttg atatatgtgg gcgtcgagcc
     1201 accgaagccc aaagccgaag tgattctggt ggcagaaaac agcccgatca aaaccgtagc
     1261 cgatcttaaa ggtcacaaag ttgcctttca gaaaggttcc agttcacaca accttttact
     1321 gcgtgcactg cgtcaggccg gacttaagtt tactgacatc cagcccactt atctgacgcc
     1381 agctgatgcc cgcgccgcgt tccagcaagg taacgttgac gcctgggcta tctgggatcc
     1441 ctactactcc gctgcgttat tacagggcgg cgtgcgggtg ctgaaagacg gcaccgatct
     1501 caatcaaact ggatcgtttt atctggcagc tcgcccctat gcagaaaaaa acggcgcttt
     1561 tattcagggc gtactggcaa cctttagtga ggccgatgcg ttaacccgca gccagcgcga
     1621 gcaaagcatc gctttactgg caaaaacgat gggcttaccg gcaccggtga ttgcctcgta
     1681 tctggatcat cgtcctccca ccaccatcaa accggtgagc gctgaagttg ccgccttaca
     1741 gcagcaaacg gcagatctgt tttatgaaaa ccgtctggtg tcgaaaaaag ttgatattcg
     1801 ccagcgcatc tggcagccca ctcacctgga aggaaaacaa ttatgagtct gaatatgttc
     1861 tggtttttac cgacccatgg tgacgggcat tatcttggaa cggaagaagg ttcacgcccg
     1921 gttgatcacg gctatctgca acaaattgcg caagcggcgg atcgtctggg ctataccggt
     1981 gtgctgatcc caacggggcg atcgtgtgaa gatgcgtggc tggtggcggc gtcgatgatc
     2041 ccggtgacgc agcggctgaa gtttcttgtc gccctgcgtc ccagcgtaac ctcacctacc
     2101 gttgccgctc gccaggccgc cacgcttgac cgtctctcaa atggtcgtgc gttgtttaat
     2161 ctggtcacag gcagcgatcc acaagagttg gcaggcgacg gcgtgttcct tgatcatagc
     2221 gagcgctacg aagcctcggc ggaatttacc caggtctggc ggggcgctta ttgctggggg
     2281 aaagcgtcga tttcaacggt aaacatattc atgtgcgggg agcaaaactg ctcttcccgc
     2341 cgattcaaca gccgtatcct ccgctttact ttggcggatc gtcagatgtc gcccaggagc
     2401 tggcggcaga acaagttgat ctctacctca cctggggcga accgccagag ctggtaaaag
     2461 agaagatcga acaagtgcgg gcgaaagctg ccgcgcatgg acgcaaaatt cgtttcggta
     2521 ttcgtctgca tgtgattgtt cgtgaaacta acgacgaagc gtggcaggcc gccgagcggt
     2581 taatctcgca tcttgatgat gaaactatcg ccaaagcaca ggccgcattc gcccggacgg
     2641 attccgtagg acaacagcga atggcggcgc tacataacgg caagcgcgac aatctggaga
     2701 tcagccccaa tttatgggcg ggcgttggct tagtgcgcgg cggtgccgga acggcgctgg
     2761 tgggcgatgg tcctacggtc gccgcgcgaa tcaacgaata cgccgcgctt ggcatcgaca
     2821 gctttgtgct ttcgggctac ccgcatctgg aagaagcgta tcgggttggc gagttgctgt
     2881 tcccacatct ggatgtcgcc attccggaaa ttccccagcc gcagccgctg aatccgcaag
     2941 gcgaagcggt ggcgaatgat tttatccccc gtaaagtcgc gcaaagctaa ggaacacgat
     3001 gatggcaacg ccagtgaaga agtggttatt gcgcgttgcc ccctggtttt taccggtggg
     3061 catcgtggcg gtgtggcaac tggcctcctc ggttggctgg ctgtcgacgc gtattttgcc
     3121 ttcaccggaa ggagtggtga cggcgttctg gacgctctcc gccagcggcg aattgtggca
     3181 gcatctggcg atcagttcct ggcgggcgct gattggcttt tcaattggcg gatcgctggg
     3241 cttgattctg ggactgatta gcgggctgtc acgctgggga gaacggctgc tggatacctc
     3301 aattcagatg ttgcgcaacg taccgcatct ggcgctgatc ccgctggtga ttttgtggtt
     3361 tggcattgat gaatccgcaa aaatctttct ggtggcgctc ggcacgctat tccccattta
     3421 tatcaacacc tggcatggca tccgtaatat cgatcgcggt ctggtggaaa tggcgcgtag
     3481 ctatggatta tccggcatac cgctgtttat ccatgtgatc ctgcctggtg ccctgccctc
     3541 aattatggtc ggcgtgcgtt ttgcgttggg cctgatgtgg ctgacgctga ttgttgccga
     3601 aaccatttct gccaattcag gcattggtta tctggcgatg aatgcgcggg agtttttgca
     3661 aacggacgtg gtggtagtcg ctattattct ttacgccctg ctcggcaaac ttgctgacgt
     3721 cagcgcgcag ctgttagagc gcctctggtt gcgctggaac ccggcttatc atttgaagga
     3781 ggccacggta tgaatactgc tcgtctgaac cagggcacac cattgttgct caatgcagta
     3841 agcaaacatt acgcggaaaa tatcgtcctg aaccaactgg atttacatat tccggcaggt
     3901 cagtttgtgg cggtggtggg ccgcagcggt ggtggcaaaa gtaccctgct gcgcctgctg
     3961 gcaggtctgg aaacgccaac cgcaggcgat gtgttagcgg gcaccacacc gctggctgag
     4021 attcaggaag acacgcgaat gatgtttcag gatgcgcgtc tgctgccgtg gaaatcggtg
     4081 attgataacg ttgggttagg ccttaaaggc cagtggcgcg atgccgcgcg tcgggcgttg
     4141 gctgcagtag gactggagaa tcgcgccggg gaatggcctg ccgcactttc tggcgggcag
     4201 aaacagcgtg tggcgctggc aagagcgttg attcatcgac cgggtttatt gttgcttgat
     4261 gaaccgctcg gggcactgga cgccttaacg cgactcgaga tgcaggattt gattgtgtca
     4321 ctttggcagc agcacggctt taccgtactg ttggtgacgc atgatgtgag cgaagcggta
     4381 gcaatggctg accgggtgtt gttaattgaa gagggaaaaa ttggtctgga tttgacagtg
     4441 gatatccccc gtccgcgtcg attagggtcg gtgcgattag cggagctgga agcggaagta
     4501 ttgcaacggg taatgcggcg tgggcattct gaacaactga ttcggcgtca tggttaatat
     4561 gccggatgtg gcgcacaaaa ccagcatttt caacggactt gtaggcctga taagacacgt
     4621 caagcatcgc atcaggcagt tgggcacggc tgccggatgc ggcgtgaacg ccttatccag
     4681 cctacaaaac cagcaatttc aacggagttg taggcctgat aagacgcgtc aagcgtcgca
     4741 tcaggcattt tggctcaaaa gtatcaagcc agtgctttgg tgatcttctc gtacagatcg
     4801 ccagagagat tttccagccc tttcaactgt tccagcgccg cgcgcatttt ctcctgacgt
     4861 ttggcatcat aacgtttcag gcgaatcagc ggttcaatca gacgtgaagc tacctgcggg
     4921 ttacggctgt tgaggtcggt aagcatttcc tccaggaact ggtaaccgct gccatcttcg
     4981 gcatggaacg ctgccgggtt gctgcccgca aacgcaccaa tcaacgaacg aatacggttc
     5041 gggttgctca tggtaaatga gcgatgctgc aacagaccac gcaccgtctc cagcacattc
     5101 gccgccgggc tggtggcttg caggataaac catttatcca tcaccagacc gtcctgatgc
     5161 cacttatcgt cgtactcctg catcagcgcg tcacggcaag gcagctgtgc agcaaccgcc
     5221 gcagaaagcg ctgccagcgc atcggtcata ttgtttgctt catggtattg cttgctcacc
     5281 agcacgtcag ccagatgcgt ttcaccaaaa gcaaggaagc gcaggcaggc atttcgcaga
     5341 gtgcgttttg caatatcttc atgctcaaca cggtactcgc tctggtaatt cgcgttgtaa
     5401 atagccagca gctcatccgc cagttcagtc gccagagtac gagtgagtgc ttcgcgtact
     5461 tcggcaatag caatcgggtc gatgatatcg aacagttcag ccatttcatt gacagaaggc
     5521 agcgtcagga tttctgccgc cagcgccgga tcaatcttct catcgagcag caccgcgcgg
     5581 aaagcgtcag ccacatgtac cggcagagac agcggctgcc cttgctgatg acgggcgacg
     5641 ttcagcttga tgtaggttgc cagcaaactt tgcgccgcat cccaacggga gaaatcatta
     5701 cgcgcatgac gcatcaggaa ggtcagttgc tgatcactcc acttatattc cagtttcact
     5761 ggcgcagaga attcgcacag cagcgcaggc accggctgga agtagacatt atcaaaaaca
     5821 aaggtctgtt ccgcctgggt gacgttcagc acggaattca ccggatgacc gcctttctgc
     5881 aacgggatca ctttgccttc gttgtcatac aattcgatgg caaacggaat atgcagcggc
     5941 tgtttttctg cctgatccgg cgtggctggc gtgcgctggc tgatggtcag ggtgtactgc
     6001 tcagtttccg gattgtagtc gtctttgacg gtcacaatcg gcgtaccgga ctggctgtac
     6061 caacggcgga aatgggagag atcaacattc gacgcatctt ccatcgcctg cacaaagtcg
     6121 tcacaggtcg ctgcgctacc atcatgacgc tcgaaataga gttgcatccc tttctggaag
     6181 ttttcttcgc ccagcagggt gtggatcata cggatcactt ccgcgccctt ctcgtaaacg
     6241 gtcagggtgt agaagttgtt catctcaatg accatgtccg ggcggattgg gtgcgccatc
     6301 gggctggcgt cttccgcaaa ctgcaatccg cgcatggtgc gcacattatt gatgcggtta
     6361 actgcgcggg aaccaaggtc agagctgaat tcctgatcgc ggaagacggt taaaccttct
     6421 ttcaggctga gctggaacca gtcgcggcag gtcactcggt taccggtcca gttatggaaa
     6481 tattcatggc cgataacgcg ttcaatatcg aggtaatctt tgtcggtggc ggtgtcggtg
     6541 cgggccagca catatttgga gttaaagata ttcagaccct tattctccat tgcgcccata
     6601 ttgaagaaat ccaccgcgac gatcatatag atgtcgaggt catactccag gccgaagcgt
     6661 tcttcatccc atttcattga gtttttcagc gaggtcatcg cccacggcgc gcgatcaagg
     6721 ttgccgcgat cgacgtacag ctccagcgcc acttcgcgac cagaacgcgt ggtaaaggtg
     6781 tcgcgcagta catcaaagtc gcctgccacc agcgcaaaca ggtagcacgg tttcgggaac
     6841 gggtcctgcc actgtaccca atggcgtccg ttttccagtt caccttgcgc aacgcggtta
     6901 ccgttggaaa gcaggaaggg atatttggtt ttatcggcaa taattttggt ggtaaaacgc
     6961 gccagcacgt ccgggcggtc gagataatac gtaatatggc ggaaaccttc ggcttcacac
     7021 tgggtgcaaa gcgcatcgcc tgactgataa agcccttcca gcgcggtatt cgccgccggg
     7081 cttatttcat taacgatctt aagcgtaaaa tgctccggca aattactgat gaccagtgcg
     7141 ccctcttctt ctttccaggc ggtccacggc tcatcattaa tatgaacaga aaccagtttg
     7201 aggtcttcgc cgttgagacg aagtggagca tctgatgcac catgacggac agcctggctg
     7261 accgcggtaa cgaccgtctt ttgcgcgtcg aggtcaaagg tcaagtcaat atcagtaatc
     7321 tggtaatctg gcgcacgata atcgtgacgg tatttggctt gtggctgttg agtcataaat
     7381 aacctttagc atcttttata gagtctggtg ttcagtctat tcctgttgcg taaatcgcgc
     7441 tatgcagaat cttcatcttt tcaggtacaa acgcctttat tgctacattt ttataacata
     7501 caccgcgtaa tgccatcgac cagaaaggtg gcatatggtg tgatcggggt tcaataaatt
     7561 gcgaaacaag gtatactcca gcagttcctg aagatgttta ttgtactaaa cgctcctgta
     7621 cgaggacgct actgcgcacc tatgacacaa ttcgcttctc ctgttctgca ctcgttgctg
     7681 gatacagatg cttataagtt gcatatgcag caagccgtgt ttcatcacta ttacgatgtg
     7741 catgtcgcgg cggagtttcg ttgccgaggt gacgatctgc tgggtattta tgccgatgct
     7801 attcgtgaac agattcaggc gatgcagcac ctgcgcctgc aggatgatga atatcagtgg
     7861 ctctctgccc tgcctttctt taaggccgac tatcttaact ggttacgcga gttccgcttt
     7921 aacccggaac aagtcaccgt gtccaacgat aatggcaagc tggatattcg cttaagcggt
     7981 ccgtggcgtg aagtcatcct ctgggaagtt cctttgctgg cggttatcag tgaaatggta
     8041 catcgctatc gctcaccgca ggccgacgtt gcgcaagccc tcgacacgct ggaaagcaaa
     8101 ttagtcgact tctcggcgtt aaccgccggt cttgatatgt cgcgcttcca tctgatggat
     8161 tttggcaccc gtcgccgttt ttctcgcgaa gtacaagaaa ccatcgttaa gcgtctgcaa
     8221 caggaatcct ggttcgtggg caccagcaac tacgatctgg cgcgtcggct ttccctcacg
     8281 ccgatgggaa cacaggcaca cgaatggttc caggcacatc agcaaatcag cccggatcta
     8341 gccaacagcc agcgagctgc acttgctgcc tggctggaag agtatcccga ccaacttggc
     8401 attgcattaa ccgactgcat cactatggat gctttcctgc gtgatttcgg tgtcgagttc
     8461 gctagtcggt atcagggcct gcgtcatgac tctggcgacc cggttgaatg gggtgaaaaa
     8521 gccattgcac attatgaaaa gctgggaatt gatccacaga gtaaaacgct ggttttctct
     8581 gacaatctgg atttacgcaa agcggttgag ctataccgcc acttctcttc ccgcgtgcaa
     8641 ttaagttttg gtattgggac tcgcctgacc tgcgatatcc cccaggtaaa acccctgaat
     8701 attgtgatta agttggtaga gtgtaacggt aaaccggtgg cgaaactttc tgacagccct
     8761 ggcaaaacta tctgccatga taaagcgttt gttcgggcgc tgcgcaaagc gttcgacctt
     8821 ccgcatatta aaaaagccag ttaatatcat cagggagcta atcggctccc tttttttacc
     8881 tttaattccg aaatctttcg ctgcatttgc gaattctgct tgtctgattg cagatgccag
     8941 gtaacatagg tatccccccc ccatttacgg gtggacaagt gtttattttt tccgactatt
     9001 aacagagaga atattatgag cgttgtgcct gtagccgacg tactccaggg ccgcgtagcc
     9061 gttgacagcg aagtcaccgt acgcggatgg gtacgtaccc gccgagattc aaaagctggc
     9121 atctccttcc tcgccgttta tgacggttcc tgctttgatc ctgtacaggc tgtcatcaat
     9181 aattctctgc ccaattacaa tgaagacgtc ctgcgtctga ccaccggctg ctcggtcatt
     9241 gtgacgggta aagtcgtggc atcgccgggc caggggcaac aatttgaaat tcaggccagc
     9301 aaggttgaag ttgctggttg ggttgaagat ccagacactt acccgatggc ggcaaaacgc
     9361 cacagcattg agtatctgcg tgaagtcgct cacctgcgtc cgcgcacaaa cctgattggt
     9421 gccgtcgcgc gcgttcgcca tacgctggcg caggcgctgc atcgcttctt taacgagcag
     9481 ggattcttct gggtttcaac gccactgatt accgcatctg ataccgaagg tgcaggcgaa
     9541 atgttccgcg tttctacgct ggatctggaa aacctgccgc gtaacgatca gggcaaagtg
     9601 gatttcgaca aagacttctt tggtaaagag tctttcctga ccgtatctgg ccagttgaac
     9661 ggcgaaacct acgcttgcgc attatccaaa atttatacct tcggcccgac tttccgtgct
     9721 gaaaactcca acaccagccg tcacctggcg gaattctgga tgctggagcc ggaagtggcg
     9781 tttgctaacc tgaacgatat cgcgggtctg gctgaagcca tgctgaaata tgtcttcaaa
     9841 gcggttctcg aagaacgcgc agacgacatg aaattcttcg ctgaacgcgt agataaagat
     9901 gccgtttcac gtctggaacg cttcattgag gccgatttcg cgcaggtgga ttacaccgac
     9961 gcagtgacca ttctcgaaaa ctgcggcagg aagtttgaaa acccggttta ctggggcgtc
    10021 gatctctctt ctgagcatga gcgttatctg gcggaagaac actttaaagc accggtagtg
    10081 gttaaaaact atccgaaaga tattaaagcg ttctatatgc gcctcaacga agacggtaaa
    10141 accgttgcgg ctatggacgt tctggctccg ggcatcggtg agatcattgg tggctcccag
    10201 cgtgaagaac gtctggacgt tctggacgag cgtatgctgg aaatgggcct gaataaagaa
    10261 gattactggt ggtatcgcga tctgcgtcgc tacggtactg ttccgcattc aggtttcggt
    10321 cttggttttg aacgcctgat tgcttacgta actggcgtgc aaaacgtgcg tgatgtgatt
    10381 ccgttcccac gtactccgcg caacgccagc ttctgatttt cttcctcaaa gagccagcgc
    10441 ctgctggctc ttttttatcc tttctgtcaa gttatctgtt tgttaagtca agcaatctat
    10501 ttgcaacccc gccataacga tcatcctgtt acggaatatt acattgcaac atttacgcac
    10561 aaaaaataac ccgcattctt attgcggatt agttttttct tagctgatag cacaattttc
    10621 atactatttt ttggcattct ggatgtc
//
LOCUS       DADMKF010000136        10430 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.138, whole genome shotgun sequence.
ACCESSION   DADMKF010000136 DADMKF010000000
VERSION     DADMKF010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10430
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.138"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            142..2010
                     /gene="kup"
                     /locus_tag="JNC24_003203"
     CDS             142..2010
                     /gene="kup"
                     /locus_tag="JNC24_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HAY9159888.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAMHFFLEYKTVSFIAL
                     GAVVLSITGVEALYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            2177..2596
                     /gene="rbsD"
                     /locus_tag="JNC24_003204"
     CDS             2177..2596
                     /gene="rbsD"
                     /locus_tag="JNC24_003204"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HAY9159889.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            2604..4109
                     /gene="rbsA"
                     /locus_tag="JNC24_003205"
     CDS             2604..4109
                     /gene="rbsA"
                     /locus_tag="JNC24_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
                     /protein_id="HAY9159890.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVIALVGENGAGKS
                     TMMKVLTGIYTRDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWKTMYAEADKLLAKLNLCFKSDKLVGDLSIGDQQMVEIAKVLSFES
                     KVIIMDEPTDALTDTETESLFRVIRELKSQGRGIVYISHRMKEIFEICDDVTVFRDGQ
                     FIAEREVASLTEDSLIEMMVGRKLEDQYPHLDKAPGDIRLKVDNLCGPGVNDVSFTLR
                     KGEILGVSGLMGAGRTELMKVLYGAQPRTSGYVTLDGHEVVTRSPQDGLANGIVYISE
                     DRKRDGLVLGMSVKENMSLTALRYFSRAGGSLKHADEQQAVSDFIRLFNVKTPSMEQA
                     IGLLSGGNQQKVAIARGLMTRPKVLILDEPTRGVDVGAKKEIYQLINQFKADGLSIIL
                     VSSEMPEVLGMSDRIIVMHEGHLSGEFTREQATQEVLMAAAVGKLNRVNQE"
     gene            4114..5079
                     /gene="rbsC"
                     /locus_tag="JNC24_003206"
     CDS             4114..5079
                     /gene="rbsC"
                     /locus_tag="JNC24_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HAY9159891.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALALGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            5104..5990
                     /gene="rbsB"
                     /locus_tag="JNC24_003207"
                     /pseudo
     CDS             5104..5990
                     /gene="rbsB"
                     /locus_tag="JNC24_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
     gene            6116..7045
                     /gene="rbsK"
                     /locus_tag="JNC24_003208"
     CDS             6116..7045
                     /gene="rbsK"
                     /locus_tag="JNC24_003208"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HAY9159892.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPALVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            7049..8041
                     /gene="rbsR"
                     /locus_tag="JNC24_003209"
     CDS             7049..8041
                     /gene="rbsR"
                     /locus_tag="JNC24_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HAY9159893.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(8007..9434)
                     /locus_tag="JNC24_003210"
     CDS             complement(8007..9434)
                     /locus_tag="JNC24_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HAY9159894.1"
                     /translation="MSDKKKRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGGAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARHTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVSFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRVYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSKPNRVPSESE"
     gene            complement(9457..10149)
                     /locus_tag="JNC24_003211"
     CDS             complement(9457..10149)
                     /locus_tag="JNC24_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HAY9159895.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLNLHQAIVDAIVQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         2550 a         2516 c         2754 g         2610 t
ORIGIN      
        1 aagggggggt atttttcttt gagccaggtt aatgtggccg catttaggag tacgattttg
       61 ccgttaatcg tgcatactgt gcgctttttt gtgggccaag ggactaagca cacatttcat
      121 atttcaacga aagactagtc tatgagcact gataataagc aatcattgcc cgcgattacc
      181 ctcgcggcga ttggagttgt ctacggcgat attggtacca gcccgttata tacacttcgt
      241 gaatgtttgt ccggccagtt tggttttggc gttgaacgcg atgccgtgtt tggcttttta
      301 tcgctgatct tctggctgct aatctttgtg gtttccatta aatatctcac cttcgtgatg
      361 cgggcagata acgccggtga gggggggatc ctgacgttga tgtcgcttgc cgggcgtaat
      421 acgtcggcgc gaaccacatc aatgctggtg attatggggc taatcggcgg cagctttttc
      481 tatggtgaag tcgtcataac acccgctatt tcggtgatgt cagccattga aggtctggaa
      541 atcgtcgccc cgcagctgga tacctggata gttcccctct caattatcgt tctcacttta
      601 ttatttatga ttcaaaagca tggcacggct atggtcggta agctgtttgc gccgatcatg
      661 ctgacctggt ttttgattct ggcaggactg gggttacgta gcattattgc taacccggaa
      721 gtgctgcatg cgctgaatcc gatgtgggcg atgcatttct tccttgaata caaaacggtt
      781 tcttttattg cattaggggc agtggtgttg tcgattacgg gggtcgaggc gctgtatgct
      841 gatatggggc actttggtaa gttccctatt cgcctggcgt ggttcaccgt cgtattacct
      901 tccttgaccc ttaattactt cggtcaggga gcgctgttgt taaagaaccc ggaagcgatt
      961 aagaacccgt tcttcctgtt ggcaccggac tgggcgctga tcccgctgct gatcatcgcc
     1021 gcactggcga cggtaattgc ctcgcaggcg gttatctctg gcgtcttctc attgacgcgt
     1081 caggcggtac gtctgggata tttgtcgccg atgcgcatta ttcacacctc cgaaatggag
     1141 tcagggcaaa tctatattcc gtttgtgaac tggatgctct atgtcgcggt cgtgattgtg
     1201 attgtcagct ttgagcactc cagcaacctg gcggcggcgt acgggattgc ggtgaccgga
     1261 accatggtgc tgacgtctat tctctcgact accgtggcac gtcagaactg gcactggaat
     1321 aagtattttg ttgcgctgat cctgattgct ttcctttgtg tcgatattcc attgttcacc
     1381 gctaacctcg ataaactgct ctccggcggc tggttgccat tgagcctcgg tactgtgatg
     1441 tttatcgtga tgaccacctg gaagagcgag cgtttccgct tgctgcggcg gatgcatgaa
     1501 catggtaact ctctggaagc gatgattgct tcgctggaga aatcaccgcc tgttcgcgtg
     1561 cccgggaccg cggtgtatat gtcgcgtgca atcaacgtca ttccctttgc gctgatgcat
     1621 aaccttaaac ataacaaggt attgcatgag agggtgattc tgttaactct gcgcaccgaa
     1681 gacgcgccat atgtccataa cgtccgtcgg gtacagattg aacaactgtc gcccactttc
     1741 tggcgcgtgg tggcaagtta tggttggcga gaaacgccaa acgtggaaga agttttccac
     1801 cgctgcggtc tggaaggatt aagttgccgg atgatggaaa cctccttctt tatgtcgcat
     1861 gagtcgttga tcctcggcaa acgcccgtgg tatttgcgtc tgcgcggcaa gctgtacttg
     1921 ctgctgcaac gtaatgcgct gcgtgcacca gatcaatttg aaatcccgcc aaacagggtt
     1981 atcgaactgg gtactcaggt cgaaatctaa cgctagacgc ctcctttctc cataaggggg
     2041 cgtttttgtt ttcatggtta atcaccatgt aaaacgtttc gaggttgatc acatttccgt
     2101 aacgtcacga tggttttccc aactcagtca ggattaaact gtggatcagc gaaacgtttc
     2161 gctgatggag aaaaaaatga aaaaaggcac cgttcttaat tctgatattt catcggtgat
     2221 ctcccgtctg ggacataccg atacgctggt ggtgtgtgat gctggtttac ccatccccaa
     2281 aagtacaacg cgtatcgata tggcattaac ccagggtgta ccttctttta tgcaggtgct
     2341 gggcgtcgtc acaaatgaaa tgcaggtcga ggctgccatt atcgcggaag agatcaaaca
     2401 acataatccg caactccacg aaacgttgct cactcacctt gagcagctgc aaaaacacca
     2461 gggaaatacc attgaaattc gttacaccac gcatgaacaa ttcaaacaac aaaccgcaga
     2521 aagtcaggcg gtaattcgca gcggagaatg ttctccgtat gcgaatatca ttctctgtgc
     2581 tggcgtgacg ttctgaggcc gtcatggaag cattacttca gcttaaaggc atcgataaag
     2641 ccttcccggg cgtaaaagcc ctctcgggcg ctgcgttaaa tgtctatccg ggccgcgtga
     2701 tagcgctggt gggcgaaaac ggcgcgggta aatccaccat gatgaaagtg cttactggta
     2761 tctatactcg cgatgccggc acgcttttat ggctggggaa agaaacgaca tttaccgggc
     2821 cgaaatcttc ccaggaagcc gggattggga ttatccatca ggaactgaac ctgatcccgc
     2881 agttgaccat tgccgaaaac attttcctcg gtcgtgagtt tgttaatcgc tttggcaaaa
     2941 ttgactggaa aaccatgtat gccgaagcgg ataaattgct ggctaaactt aacctgtgct
     3001 ttaaaagcga caagctggtg ggcgatcttt ccatcggtga ccagcaaatg gttgaaatcg
     3061 ccaaagtgct gagctttgag tcgaaagtca tcattatgga tgaaccgacc gatgcgctga
     3121 ccgataccga aaccgaatcc ctgttccgcg tcatccgcga gctgaaatcg caaggccgcg
     3181 gtattgtcta tatctcccac cgcatgaaag aaatcttcga gatttgcgat gacgttaccg
     3241 tttttcgtga cgggcaattt attgctgagc gcgaagtggc atcactgacc gaagattcgc
     3301 tgattgagat gatggtgggc cgcaagctgg aagatcaata tccacacctg gacaaagcgc
     3361 cgggagatat ccgcctgaaa gtcgataatc tctgcggacc tggcgttaac gatgtctctt
     3421 ttactttacg caaaggcgaa attcttggcg tctctggttt gatgggcgca ggtcgtaccg
     3481 aactgatgaa agtgctctac ggcgcacagc cgcgcaccag cggttacgtc accctggatg
     3541 ggcatgaagt cgttacccgt tcaccgcagg atggcctggc aaacggcatt gtgtatatct
     3601 ccgaagaccg taaacgtgac ggtttagtgt tgggcatgtc agtaaaagag aacatgtcgc
     3661 tgacagcgct gcgctacttc agccgcgctg gcggcagttt gaagcatgcc gatgaacagc
     3721 aggcagtgag tgatttcatt cgtctgttta atgtgaaaac accgtcgatg gaacaggcaa
     3781 ttggtctgct ttccggtggc aatcagcaaa aagtggcgat tgcccgcggt ctgatgacac
     3841 gccccaaagt gttgatcctt gatgaaccta cccgtggcgt agatgtcggc gcgaaaaaag
     3901 agatctatca actgattaac cagttcaaag ccgatggctt gagcatcatt ctggtgtcat
     3961 cggagatgcc agaagtatta ggcatgagcg atcgcatcat cgtcatgcat gaagggcatc
     4021 tcagcgggga atttactcgt gagcaggcca cccaggaagt gttaatggct gccgctgtgg
     4081 gcaagcttaa tcgcgtgaat caggagtaaa aaaatgacaa cccagactgt ctctggtcgc
     4141 cgttatttca cgaaagcgtg gctgatggag cagaaatcgc ttatcgctct gctggtgctg
     4201 atcgcgattg tctcgacgtt aagcccgaac tttttcacca tcaataactt attcaatatt
     4261 ctccagcaaa cctcggtgaa cgccattatg gcggtcggga tgacgctggt gatcctgacg
     4321 tcgggcatcg acttgtctgt aggttctctg ttggcgctaa ccggcgcagt tgctgcatct
     4381 atcgtcggca ttgaagtcaa tgcgctggtg gctgtcgctg ctgctctcgc gttaggtgcc
     4441 gcaattggtg cggtaaccgg ggtgattgta gcgaaaggtc gcgttcaggc gtttatcgct
     4501 acgctggtta tgatgctttt actgcgcggc gtgaccatgg tttataccaa cggtagccca
     4561 gtgaataccg gctttactga gaacgccgat ctgtttggct ggtttggtat tggtcgtccg
     4621 ctgggcgtac cgacgccagt ctggatcatg gggattgtct tcctcgcggc ctggtacatg
     4681 ctgcatcaca cgcgtctggg gcgttacatc tacgcgctgg gcggcaacga agcggcaacg
     4741 cgtctttctg gtatcaacgt caataaaatc aaaatcatcg tctattctct ttgtggtctg
     4801 ctggcatcgc tggccgggat cattgaagtg gcgcgtctct cctccgcaca acccacggcg
     4861 gggactggct atgagctgga tgctattgct gcggtggttc tgggcggtac gagtctggcg
     4921 ggcggaaaag gtcgcattgt tgggacgttg atcggcgcat taattcttgg cttccttaat
     4981 aatggattga atttgttagg tgtttcctcc tattaccaga tgatcgtcaa agcggtggtg
     5041 attttgctgg cggtgctggt agacaacaaa aagcagtaat aacgactaca ggacatcttg
     5101 aatatgaaca tgaaaaaact ggctaccctg gtttccgctg ttgcgctaag cgccaccgtc
     5161 agtgcgaatg cgatggcaaa agacaccatc gcgctggtgg tctccacgct taacaacccg
     5221 ttctttgtat cgctgaaaga tggcgcgcag aaagaggcgg ataagcttgg ctataacctg
     5281 gtggtgctgg actcccagaa caacccggcg aaagagctgg cgaacgtgca ggacttaacc
     5341 gttcgcggca ccaaaattct gctgattaac ccgaccgact ccgacgcagt aggtaatgct
     5401 gtgaagatgg ctaaccaggc gaacatcccg gttatcactc ttgaccgcca ggcaacgaaa
     5461 agtgaagtgg tgagccacat tgcttctgat aacgtactgg gtggcaaaat cgccggtgat
     5521 tacatcgcga agaaagcggg tgaaggtgcc aaagttatcg agctgcaagg cattgctggt
     5581 acatccgcag cccgtgaacg tggcgaaggc ttccagcagg ccgttgctgc tcacaagttt
     5641 aatgttcttg ccagccagca gattttgatc gcactaaagg tttgaacgta atgcagaacc
     5701 tgttgaccgc tcatccggat gttcaggctg tattcgcgca gaatgatgaa atggcgctgg
     5761 gcgcgctgcg cgcactgcaa actgccggta aatcggatgt gatggtcgtc ggatttgacg
     5821 gtacaccgga tggcgaaaaa gcggtgaatg atggcaaact agcagcgact atcgctcagc
     5881 tacccgatca gattggcgcg aaaggcgtcg aaaccgcaga taaagtgctg aaaggcgaga
     5941 aagttcaggc taagtatccg gttgatctga aactggttgt taagcagtag ttttaatcag
     6001 gttgtatgac ctgatggtga cataaatacg tcatcgacag atgaacgtgt aatataaaga
     6061 aaagcagggc acgcgccacc ctaacacggt ggcgcatttt atggacatcc cgaatatgca
     6121 aaacgcaggc agcctcgttg ttcttggcag cattaatgct gaccacattc ttaatcttca
     6181 atcttttcct actccaggcg aaaccgtaac cggtaaccac tatcaggttg catttggcgg
     6241 caaaggcgcg aatcaggctg tggctgctgg gcgtagcggt gcgaatatcg cgtttattgc
     6301 ctgtacgggt gatgacagca ttggtgagag cgttcgccag cagctcgcca ctgataacat
     6361 tgatattact ccggtcagcg tgatcaaagg cgaatcaaca ggtgtggcgc tgatttttgt
     6421 taatggtgaa ggtgagaatg tcatcggtat tcatgccggc gctaatgctg ccctttcccc
     6481 ggcgctggtg gaagcgcaac gtgagcgtat tgccaacgcg tcagcattat taatgcagct
     6541 ggaatcacca ctcgaaagtg tgatggcagc ggcgaaaatc gcccatcaaa ataagactat
     6601 cgttgcgctt aacccggctc cggctcgcga acttcctgac gaactgctgg cgctggtgga
     6661 cattattacg ccaaacgaaa cggaagcaga aaagctcacc ggtattcgtg ttgaaaatga
     6721 tgaagatgca gcgaaggcgg cgcaggtact gcatgaaaaa ggtatccgta ctgtactgat
     6781 tactttagga agtcgtggtg tatgggctag cgtgaatggt gaaggtcagc gcgttcctgg
     6841 attccgggtg caggctgtcg ataccattgc tgccggagat acctttaacg gcgcgttaat
     6901 cacggcgttg ctggaagaaa aaccattgcc agaggcgatt cggtttgccc atgctgccgc
     6961 tgcgattgcc gtaacacgta aaggcgcaca accttccgta ccgtggcgtg aagagatcga
     7021 cgcattttta gacaggcaga ggtgacgctt ggctacaatg aaagatgttg cccgcctggc
     7081 gggcgtttct acctcaacag tttctcacgt tatcaataaa gatcgcttcg tcagtgaagc
     7141 gattaccgcc aaagttgaag cggcgattaa agaactcaat tacgcgccat cagccctggc
     7201 gcgtagcctc aaactcaatc aaacacatac cattggcatg ttgatcactg ccagtaccaa
     7261 tcctttctat tcagaactgg tgcgtggcgt tgaacgcagc tgcttcgaac gcggttatag
     7321 tctcgtcctt tgcaataccg aaggcgatga acagcggatg aatcgcaatc tggaaacgct
     7381 gatgcaaaaa cgcgttgatg gcttgctgtt actgtgcacc gaaacgcatc aaccttcgcg
     7441 tgaaatcatg caacgttatc cgacagtgcc tactgtgatg atggactggg ctccgttcga
     7501 tggcgacagc gatcttattc aggataactc gttgttgggc ggagacttag caacgcaata
     7561 tctgatcgat aaaggtcata cccgtatcgc ctgtattacc ggtccgctgg ataaaactcc
     7621 ggcgcgcctg cggttggaag gttatcgggc ggcgatgaaa cgtgcgggtc tcaacattcc
     7681 tgatggctat gaagtcactg gtgattttga atttaacggc gggtttgacg ccatgcgcca
     7741 actgttatca catccgctgc gtcctcaggc cgtctttacc ggaaatgacg ctatggctgt
     7801 tggcgtttac caggcgttat atcaggcaga gttacaggtt ccgcaggata tcgcggtgat
     7861 tggctatgac gatatcgaac tggcaagctt tatgacgcca ccattaacca ctatccacca
     7921 accgaaagat gaactggggg agctggcgat tgatgtactc atccatcgga taacccagcc
     7981 gacccttcag caacaacgat tacaacttac tccgattctg atggaacgcg gttcggctta
     8041 gatttacgct gtcttttgat caaattatta ccatcggttg ttttcagaag catgaacatt
     8101 gctgctgaag caacagtaat aatgcccatc gtgataaacg tatagtggaa ttgttcgaca
     8161 gtcgttgtgc cttccatccc ttcataaacg cgaaggacgg ccgcacttac agcaacgcct
     8221 aaactaatcg acagttgctg cgtgaccgcc agaacactgt taccgctgct ggcgttgtca
     8281 tcggtcagat cggcaagtgt gatggtattc atcgcggtaa attgcgtcga catagccatc
     8341 cctaatataa acaacggcaa gatcagcatc catatcgcca ttgccggtga ttgcaaagag
     8401 aactgagcga tcattagccc aataatcacc gtgatcccca ctaacgtatg gcgatagccc
     8461 agacgacgta agacttgggt aaccatcgat tttgcaataa tggaacctaa cgctgtcggt
     8521 gccatcatac agccggcaat aaacgcctga taaccaaatc ctacctgtaa catcaatggc
     8581 ataaggaacg atacacaacc ggtccccaga cgggttgcaa tattgcctac gataccgatt
     8641 gagaaagtgc gggttttaaa taaatctaat gaaattaatg ggtttggcgt gtgtcgcgca
     8701 tggagaatat agagaagcag taacccgatg ctggtgacaa ttaccgtcaa ggcaatccag
     8761 ctggcgacaa tcttttcccc gaatagctct attccgcttg agaagagaac aaggctgagg
     8821 ccaaacagca aaaagccagt gatatcgaat ctgcgtcgtg cggtggtgaa attgggcata
     8881 tgtttgcgcg cgtaaagaag gcccgcaata cctatgggga tattgattaa aaatatccag
     8941 tgccaggttg cccaggtgac cagcacgccg ccaagaacgg ggcctaaaat tggccccacc
     9001 agacccggca tggcgacaaa attcaatact ggaagaagtt cattacgagg ataagcgcgc
     9061 agtaaggcca gccgagcaac aggcatcatc attgcgccgc ctatcccctg aataacccgg
     9121 aagacaacca gctgtggtag cgaattagaa agtgcgcagg ccagagaacc caatgtgaac
     9181 agactcacgg caagggtaaa aatgcgacgc gtaccgaagc gatcggctag ccatccgctt
     9241 accggaataa gcatcgccac cgtcagcgta taactgatga tggctgattg catcgcgaga
     9301 ggagaacgat taaggctatg agcgattgcg ggtaaggcgg tattcagaat agtggcatca
     9361 agtgcctgca tgaagaaggc catcgccgcg atccacggca aacccgccat actgcgcttc
     9421 tttttatcgc tcattcaatg tcttgttatc gggttgtcac ttatcaggtg agcgtagcag
     9481 cgcctgacaa gctttaaatg ccgcgtcgcc atcgctttgg acaatcgcat cgacaatcgc
     9541 ctggtgcaga tttagcttta tcactgtgtc gctggtaatt gacgtgaagt aagtgtgata
     9601 aaccgaatgg aatagcgagg cgaatgaggt caaaaacgga ttggcgctca tttcatagat
     9661 atgctcatgc caggccatat cgacttcgat ccagcgttca cggcgaaagt tctcttttaa
     9721 tgccgccatt tcggccatta acgtattgag atgcgccttc tgttccgcgg tgccaaccgt
     9781 tgctgccagt aggcaggctt gcggctccag acagatacgc ataaccagaa agtgatcgat
     9841 gacctgatga aagttctctt ctgtcatcca ccaggtaagc aattcctgat caagaaaatt
     9901 ccagttcgat tgtggcatga cccgagtacc aattcgcggt cgcggtaaaa ccatcccttt
     9961 tgccgttaac gttttgaccg cttcgcgtac cgctgtacga ctcactccaa attgctcgcc
    10021 cagctcaatc tcaccaggca aaatggtgcc gggttcatat tcacctttta agatccgttg
    10081 cgccagcttc tcagccagaa catacgaaag gtttttctgt gcagctaact gttgtgcgct
    10141 taaaggcatt acttatcttc ctttttcttt ttattcctcc ttagtatgcc accaggaagt
    10201 gtgattacgg ttgcaaaaac ggcaaattgc ttgttttatg gtacattaac ggggcttttg
    10261 ctgaaaaaat gcgcggtcag aaaattattt taaatttcct cttgtcaggc cggaataact
    10321 ccctataatg cgccaccact gacacggaac aacggcaaac acgccgccgg gtcagcgggg
    10381 ttctcctgag aatctcaaca gagaaagcaa aaataaatgc ttgactctgt
//
LOCUS       DADMKF010000137        10172 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.139, whole genome shotgun sequence.
ACCESSION   DADMKF010000137 DADMKF010000000
VERSION     DADMKF010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10172
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.139"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            123..611
                     /locus_tag="JNC24_003212"
                     /pseudo
     CDS             123..611
                     /locus_tag="JNC24_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001341229.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rtcB domain protein"
     gene            608..1222
                     /locus_tag="JNC24_003213"
     CDS             608..1222
                     /locus_tag="JNC24_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HAY9159896.1"
                     /translation="MILLQLSSAQGPEECCLAVKKALERLIKEAARQDVAVTVLETET
                     GRYSDTLRSALVSLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAVLKSQRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(1279..2736)
                     /gene="pepD"
                     /locus_tag="JNC24_003214"
     CDS             complement(1279..2736)
                     /gene="pepD"
                     /locus_tag="JNC24_003214"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008783788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HAY9159897.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKSVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDVL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            2997..3455
                     /gene="gpt"
                     /locus_tag="JNC24_003215"
     CDS             2997..3455
                     /gene="gpt"
                     /locus_tag="JNC24_003215"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HAY9159898.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            3547..4791
                     /gene="frsA"
                     /locus_tag="JNC24_003216"
     CDS             3547..4791
                     /gene="frsA"
                     /locus_tag="JNC24_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HAY9159899.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     IQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            4849..5250
                     /locus_tag="JNC24_003217"
     CDS             4849..5250
                     /locus_tag="JNC24_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HAY9159900.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(5289..6344)
                     /gene="phoE"
                     /locus_tag="JNC24_003218"
     CDS             complement(5289..6344)
                     /gene="phoE"
                     /locus_tag="JNC24_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HAY9159901.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAVGMTYQF"
     gene            6632..7735
                     /gene="proB"
                     /locus_tag="JNC24_003219"
     CDS             6632..7735
                     /gene="proB"
                     /locus_tag="JNC24_003219"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HAY9159902.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            7747..9000
                     /gene="proA"
                     /locus_tag="JNC24_003220"
     CDS             7747..9000
                     /gene="proA"
                     /locus_tag="JNC24_003220"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HAY9159903.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESVEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALAQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            9115..9190
                     /locus_tag="JNC24_003221"
     tRNA            9115..9190
                     /locus_tag="JNC24_003221"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9148..9150,aa:Thr,seq:cgt)
BASE COUNT         2521 a         2457 c         2640 g         2554 t
ORIGIN      
        1 ctattttaca gcggcatatt gcctcgtttt cgcatcatgg tttgcctgaa ggcagtgacg
       61 acgcgctacg gtatattgcg gaacatgacg atgcgctggc gtttgcgcgt attaatcgcc
      121 agctgatctc tttacgcata atgcaacagg ttaaggccac cggaagtccg gttctggatg
      181 tggcgcataa ctttgttagc gcgtgtcaaa tcggcgatca acagggctgg ttgcatcgta
      241 aaggtgccac gccggatgac aacggtctgg tgattattcc cggttcactc ggtgattact
      301 cctagcttgt acagcctgtt gcgaacgaaa aaacgctcca ctcgctggcg catggggctg
      361 ggcgtaaatg ggggcgcacc gagtgtaaag ggcgtctggc agcgaaatac acagcgacgc
      421 agctctcacg tactgaactt ggcagtcggg taatttgtcg cgataaacaa ctcatctttg
      481 aagaagcgcc acaagcctat aaatcggctg aaagcgtggt gcaatgtctg gtgcaggctg
      541 gtttaattat tcctgtcgcg cggctgcgtc cggtgctaac gctcaaaaac agtggaggga
      601 aaaaaggatg atcttgctac aactctcctc agcgcagggg ccggaagaat gttgtctcgc
      661 agtgaaaaag gcgctggagc gattaattaa agaagctgcc cgacaagacg tcgcggtaac
      721 ggtgttggaa acagaaacgg gccgctactc tgacacgctg cgttcagctc tggtttctct
      781 ggatggcgat aacgcatggg cattaagcga aagctggtgc ggcactattc agtggatttg
      841 tccgagtccg tatcggcctc atcatgggcg caaaaactgg tttctgggca ttgggcgttt
      901 taccgctgat gagcaggaac aatcggatgc aatccgttat gagacgttgc gttcgtcggg
      961 gccgggcggt caacatgtca ataaaaccga ctcggcggta cgcgccacgc atttggcatc
     1021 cggtattagc gtgaaggttc agtcagagcg tagtcagcat gctaacaagc ggctggcacg
     1081 attgctgatt gcctggaagc tggagcagca gcaacaggaa aatagcgcgg tgctgaaatc
     1141 gcagcggcga atgttccatc accagattga acgtggcaac ccgagacgga catttacagg
     1201 gatggctttt atcgaaggat aatgaaggat gaaactgccg gaaggcgatt aaacgccatc
     1261 cggcagcaaa tcaaataatt acttcgccgg aatttctttc agcagttcag tcagcagtgt
     1321 ccagtaatga cctacgcttt cgatgtgaac ttgctcatcc ggagagtgtg gaccggtgat
     1381 agttggcccg atagaaacca tgtccatttc cggatacggt tttttgaaca gaccacattc
     1441 caggcccgcg tggataatct ggatgttcgg cgttttgttg aacagacgct gataggtttc
     1501 acgtaccaga tgcatcaccg gagaattagc gtccggctgc cagccaggat atgcgccttt
     1561 cgcttcggtt ttcgcgccag ccagtttacc cagcgaatcc agcatgctca ccacgtagtc
     1621 tttaccgctg tcgatcagag aacggatcag acagtgaatt tctacgttat tatcagtcat
     1681 ggtcaccaca ccgacgttca gggaggtttc gaccacacct tttgccacgt ctgagttgcg
     1741 gatcacaccg ttcggggtgg cgttcagcag acgaataaag gtatcgcgag atttcgcaat
     1801 cagggcagct ttatcgttcg ctacagagtc cagcaacaag gccagatttt tctctttctc
     1861 tgccagctcg tttttcagga tctcctgata ggtattcacc agagatttca ggacgtcgac
     1921 tttatcagct gcgacagcaa tggtcgcaaa ggcttcacgc gggatggcgt tacgcagtgt
     1981 gccgccgttg aaatcgataa ggcgcagatc cagttcttct gcatgacccg ctaggaagcg
     2041 caccagcagt ttgttggcat tacccaggcc aacgtggatt tccccgccgg agtgaccccc
     2101 tttcagacct tttaaggtta acttgaaggt ttcaaaacca gctggaaccg cttcacgatc
     2161 taaatgcagg ttggaggtga agtcgatacc ccccgcacaa cccatgtaga tttcaccttc
     2221 ttcttcggag tcggtgttaa tcagaatatc agcctgcaac cagttgctct gtaagccgaa
     2281 cgcaccgtcc ataccggctt cttcggtcat ggtcagcagc acttccagcg ggccgtgaac
     2341 cacgttttcg tcagccagaa ccgccagcgc agacgccata ccaatgccgt tatctgcacc
     2401 cagcgtggtg ccgcgcgctt taacccattc gccatcaata taaggctgga taggatcttt
     2461 cgtgaagtca tgcacggtgt cgttattttt ctgcggcacc atatcgaggt gggcctgtaa
     2521 gacgaccgat ttacgatttt ccatacctgc ggtagcaggt ttacgaatca ggatattacc
     2581 tacctgatcg cgttcgacat ggaaaccttt ctcttttgcc caaccaacaa tgtattcagc
     2641 gagttgctct tcatgatagg acgggtgagg aatagaacag attttggcaa aaatatccca
     2701 cagcggctgt ggagataatt gagacagttc agacacgtta agtctccttg tcgatcaccc
     2761 gcaaaacagt attgcaggtc acagggttag cagaaaatgt tgtcaacaca agacaggctt
     2821 gcgagatatg tttgagaata ccactttatc ccgcgtcagg gagaggcagt gcgtaaaaag
     2881 acgcggactc atgtgaaata ctggttttta gtgcgccaga tctctataat ctcgcgcaac
     2941 ctattttccc ctcgaacact ttttaagccg tagataaaca ggctgggaca cttcacatga
     3001 gcgaaaaata catcgtcacc tgggacatgt tgcagatcca tgcacgtaaa ctcgcaagcc
     3061 gactgatgcc ttctgaacaa tggaaaggca ttattgccgt aagccgtggc ggtctggtac
     3121 cgggtgcgtt actggcgcgt gaactgggta ttcgtcatgt cgataccgtt tgtatttcca
     3181 gctacgatca cgacaaccag cgcgagctta aagtgctaaa acgcgcagaa ggcgatggcg
     3241 aaggcttcat cgttattgat gacctggtgg ataccggtgg tactgcggtt gcgattcgtg
     3301 aaatgtatcc aaaagcgcac tttgtcacca tcttcgcaaa accggctggt cgtccgctgg
     3361 ttgatgacta tgttgttgat atcccgcaag atacctggat tgaacagccg tgggatatgg
     3421 gcgtcgtatt cgtcccgcca atctccggtc gctaatcttt tcaacgcctg gcactgccgg
     3481 gcgttgttct ttttaacttc aggcgggtta caatagtttc cagtaagtat tctggaggct
     3541 gcatccatga cacaggcaaa cctgagcgaa accctgttca aaccccgctt taaacatcct
     3601 gaaacctcga cgctagtccg ccgctttaat cacggcgcac aaccgcctgt gcagtcggcc
     3661 cttgatggta aaaccatccc tcactggtat cgcatgatta accgtctgat gtggatctgg
     3721 cgcggcattg acccacgtga aatcctcgac gtccaggcgc gtattgtgat gagcgatgcc
     3781 gaacgtaccg acgatgattt atacgatacg gtgattggct accgtggtgg caactggatt
     3841 tatgagtggg ccatccaggc gatggtgtgg caacaaaaag cctgtgcgga agaagatccg
     3901 caactcagtg gtcgtcactg gctgcatgcg gctacgttgt acaacattgc tgcctatcct
     3961 catctgaaag gagatgacct ggccgagcaa gcgcaggctt tgtcaaaccg tgcctatgaa
     4021 gaagccgccc agcgtctgcc gggcacgatg cggcagatgg agtttaccgt acccggcggt
     4081 gcgcccatca ccggcttttt gcatatgccg aaaggcgatg gcccgttccc gacagtatta
     4141 atgtgtggtg gtctggatgc gatgcagacg gactattaca gcctgtatga acgttatttt
     4201 gcgccgcgcg gcattgcgat gctgactatt gatatgccgt cggtgggctt ttcatcaaaa
     4261 tggaagctca cccaggactc cagcctgttg catcagcacg ttttaaaggc tctgcctaac
     4321 gtaccgtggg tggatcacac tcgcgtcgcg gcctttggtt tccgtttcgg cgctaacgtt
     4381 gccgtgcgtc tggcatatct tgaatcgccg cgtctgaaag cggttgcctg tcttggtccg
     4441 gtagttcata ccctgttgag tgattttaag tgccagcaac aggtgccgga aatgtatctt
     4501 gacgttctgg cgagtcgttt ggggatgcat gatgcttccg acgaagcgtt acgcgtggag
     4561 ctgaatcgct attcattaaa agtgcaagga ttgctggggc gtcgctgccc aacgccaatg
     4621 ttatcaggct actggaagaa cgatccgttc agcccggaag aggactcacg cttaatcacc
     4681 tcatcatctg ctgacggtaa attattagag atcccattta acccggtgta tcggaatttt
     4741 gacaaaggtc ttcaggaaat caccggctgg atcgaaaaac gcttgtgtta aaaatttgct
     4801 aaaatttgcc aatttggtaa aacagttgca tcacaacagg agattgcaat gacgttaccg
     4861 agtggacacc cgaagagcag attgatcaaa aaatttaccg cactaggccc gtatattcgt
     4921 gaaggtaagt gcgaagataa tcgattcttt ttcgattgtc tggctgtatg cgtcaacgtg
     4981 aaaccggcac cggaagtgcg tgagttctgg ggctggtgga tggagcttga agcgcaggaa
     5041 tcccgtttta cctacagtta ccagtttggt ctgttcgata aagcaggcga ctggaagagt
     5101 gttccggtaa aagacactga agtggttgaa cgactggagc acaccctacg tgagtttcac
     5161 gagaagctgc gtgaactgct gacgacgctg aatctgaagc tggaaccggc ggatgatttt
     5221 cgtgatgaac cggtgaagtt aacggcgtga gtgaaatgtg ccggatgtat cacatccggc
     5281 aatatccatt aaaactgata ggtcatgcca accgcgacaa tatcatcatt attaatattc
     5341 aatttgttat cgctatccag ttggttgatt ttataatcaa caaacgctga catatttttg
     5401 ttgaaataat acgtagcacc gacgtcgata taattgacca gatcttcatc accgatacct
     5461 tcaatatctt tccctttcga taagacataa cccagcgatg gacgcagacc aaagtcaaac
     5521 tggtattgag cgaccgcttc aaagttctgt gtcttattgg caaagccgcc agttattggc
     5581 gtcattttgc gtgtttcaga atagaaagtt gccagataaa tattattggc atcgtatttc
     5641 agacctgttg cccatgcttc tgcacgcttg cctgtgccac ggctttgcag gttctgctcg
     5701 ttggtgcgat ctgagttggt ataggcccca ctaatggcga aatcgctgcc gccaaagtca
     5761 tatgtcaatg acgtgccgaa gccatcgccg ttttgctttt taacgtcgcg gttttcgttt
     5821 ttcccttgat attgcagggt taagttcagg ccatcgataa cgccgaagaa gtcggtgttc
     5881 cgatacgtcg ccagaccgct ggcgcgtttg gtcataaagt tgtcggtctg cgccgaggaa
     5941 tcgccaccaa attccgggaa catatcggtc caggcttcca cgtcatacaa cgcgcccagg
     6001 ttacgaccat aatcgaaaga acccaaatct ttatatttca acccggcaaa agcgagacgc
     6061 gttttctgct gtgcagtatc actctccgct ttatttccgg caaactccgc ttcccaccga
     6121 ccataaccag tcagttgatc gttaatttgt gtttcacctt taaaaccaaa acggatataa
     6181 ctctggtcgc catctttact ggcgttatca ctcatataat gcatagcttt aactttgcca
     6241 tagacatcca gtttattacc gtctttatta tatatttctg cagcctgtac agatgcagat
     6301 gccacaatgc ccatcaccac taatgccaga gtgctctttt tcattttcat tcctgatttt
     6361 aattaacgcg cgaatattca gcgggagagc cccgttgaaa acaggaaagt ttttaacctg
     6421 agattgttaa agatatatta cagattaata atattcttaa aatgtggtaa tttattaaat
     6481 ctgtaataaa agcgtaaaca actgccgcta ggcttgctga tcccgcgcaa caaaacgcca
     6541 tgctttgctc gcagatggtt ggcaaccgac gacagtcctg ctaaaacgtt cgtttgatat
     6601 catttttcct aaaattgaat ggcagagaat catgagtgac agccagacgc tggtggtaaa
     6661 actcggcacc agtgtgctaa caggcggatc gcgccgtctg aaccgtgccc atattgttga
     6721 acttgttcgc cagtgcgcgc agttacatgc cgccgggcat cggattgtta ttgtgacgtc
     6781 gggcgcgatc gccgccggac gtgagcacct gggttacccg gaactgccag cgactatcgc
     6841 ctcgaaacaa ctgctggcag cggtagggca gagtcgactg attcaactgt gggaacagct
     6901 gttttcgatt tatggcattc acgtcgggca aatgctgctg acccgtgctg atatggaaga
     6961 ccgtgaacgc ttcctgaacg cccgcgacac cctgcgtgcg ttgctcgata acaatatcgt
     7021 tccggtaatc aatgagaacg atgctgtcgc tacggcagag attaaagtcg gcgataacga
     7081 taacctttct gcgctggcgg cgattctggc gggtgccgat aaactgttgc tgctgaccga
     7141 tcaaaaaggt ttgtataccg ctgacccgcg cagcaatccg caggcagaac tgattaaaga
     7201 tgtttacggc attgatgacg cactgcgcgc gattgccggt gacagcgttt caggcctcgg
     7261 aactggcggc atgagtacca aattgcaggc cgctgacgtg gcttgccgtg cgggtatcga
     7321 caccattatt gccgcgggca gcaagccggg cgttattggt gatgtgatgg aaggcatttc
     7381 cgtcggtacg ctgttccatg cccaggcgac tccgcttgaa aaccgtaaac gctggatttt
     7441 cggtgcgccg ccggcgggtg aaatcacggt agatgaaggg gcaactgccg ccattctgga
     7501 acgcggcagc tccctgttgc cgaaaggcat taaaagcgtg actggcaatt tctcgcgtgg
     7561 tgaagtcatc cgcatttgca acctcgaagg ccgcgatatc gcccacggcg tcagtcgtta
     7621 caacagcgat gcattacgcc gtattgccgg acaccactcg caagaaattg atgcaatact
     7681 gggatatgaa tacggcccgg ttgccgttca ccgtgatgac atgattaccc gttaaggagc
     7741 tggccgatgc tggaacaaat gggcattgct gcgaagcaag cctcgtataa attagcgcaa
     7801 ctctccagcc gcgaaaaaaa tcgcgtgctg gaaaaaatcg ccgatgaact ggaagcacaa
     7861 agcgaaatca tcctcaacgc taacgcccag gatgttgctg acgcgcgagc caatggcctt
     7921 agcgaagcga tgcttgaccg tctggcactg acgcccgcac ggctgaaagg cattgccgac
     7981 gatgtacgtc aggtgtgcaa cctcgccgat ccggtggggc aggtaatcga tggcggcgta
     8041 ctggacagcg gcctgcgtct tgagcgtcgt cgcgtaccgc tgggggttat tggcgtgatt
     8101 tatgaagcgc gcccgaacgt gacggttgat gtcgcttcgc tgtgcctgaa aaccggtaat
     8161 gcggtgatcc tgcgcggtgg caaagaaacg tgtcgcacta acgctgcaac ggtggcggtg
     8221 attcaggacg ccctgaaatc ctgcggctta ccggcgggtg ccgtgcaggc gattgataat
     8281 cctgaccgtg cgctggtcag tgaaatgctg cgtatggata aatacatcga catgctgatc
     8341 ccgcgtggtg gcgctggttt gcataaactg tgccgtgaac agtcgacaat cccggtgatc
     8401 acaggtggta taggcgtatg ccatatttac gttgatgaaa gtgtagagat cgctgaagca
     8461 ttaaaagtga tcgtcaacgc gaaaactcag cgtccgagca catgtaatac ggttgaaacg
     8521 ttgctggtga ataaaaacat cgccgatagc ttcctgcccg cattaagcaa acaaatggcg
     8581 gaaagcggcg tgacattaca cgcagatgca gctgcactgg cgcagttgca ggcaggccct
     8641 gcgaaggtgg ttgctgttaa agccgaagag tatgacgatg agtttctgtc attagatttg
     8701 aacgtcaaaa tcgtcagcga tcttgacgat gccatcgccc atattcgtga acacggcaca
     8761 caacactccg atgcgatcct gacccgcgat atgcgcaacg cccagcgttt tgttaacgaa
     8821 gtggattcgt ccgctgttta cgttaacgcc tctacgcgtt ttaccgacgg cggccagttt
     8881 ggtctgggtg cggaagtggc ggtaagcaca caaaaactcc acgcgcgtgg cccaatgggg
     8941 ctggaagcac tgaccactta caagtggatc ggcattggtg attacaccat tcgtgcgtaa
     9001 ataaaaccgg gtgatgcaaa agtagccatt tgattcataa ggccattgac gcatcgcccg
     9061 gttagtttta accttgtcca ccgtgattca cgttcgtgaa catgtccttt cagggccgat
     9121 atagctcagt tggtagagca gcgcattcgt aatgcgaagg tcgtaggttc gactcctatt
     9181 atcggcacca ttaagaaaat taataactta gcttaacttc tctaagaaat tgtatcttct
     9241 gttatcactt acaaacgcct atacgttgtt atgggctttg gtagtgtaac acgtatgggc
     9301 gagtagttcc caggctcaac ctcgtaacaa gttcctttga agctggaaga cgtgccaccg
     9361 tctctaaacc ttctaccaaa actcttagca agatgttcca cacctggcgc ggtgtccaat
     9421 gatgctacac gtcgtgagac gttgcagagg ataaacaggc taaatgatgg gaagagtttt
     9481 aagtgcaata gtggattaaa gcccacctct tcaaaaccta acttgatcgg gaaagaatca
     9541 agctctagca cggtttcgtc caccggagta caacgggtgc ctgataagag aagaactgct
     9601 aagacttcat agcttttgtg aaaaagccct ttttagtgga tacgcctacg gcgcttattt
     9661 tttggtataa ccatcaaagg cgctttatgg ctcctattgt cttgaaaatg tatctttact
     9721 tgtattgtgt tttatgcgta gtctccctgt gtagtatgta acttgttaat tttcatatga
     9781 atacagaggc acacgatgaa aaacattgca gcaatcaaac gtaataaccg caagattcac
     9841 gctcgtaagt tcctgtctac gccagaagga aaagcctggc tagagcgtaa acagagagag
     9901 aacgaagaaa gaaaactcct tagtgagttg aaatggctta gagaggattt ctgaaaatct
     9961 ccagaacgca acaggacgca ttctaacgct gttttcatta ctcggtatag ggataggtga
    10021 tgtgtgttta atgcgtctgt gcgcttgttt tgtgccttat tttgcatttt cataatgcgg
    10081 aagagaacac ggaaaacctt aaaattaatt attggggtat ttaaggctat tttcaggtga
    10141 tttaagggta ttaaacttga tttaattgat ta
//
LOCUS       DADMKF010000138         9879 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.141, whole genome shotgun sequence.
ACCESSION   DADMKF010000138 DADMKF010000000
VERSION     DADMKF010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9879
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.141"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            <1..191
                     /gene="bdm"
                     /locus_tag="JNC24_003222"
     CDS             <1..191
                     /gene="bdm"
                     /locus_tag="JNC24_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="biofilm-dependent modulation protein"
                     /protein_id="HAY9159904.1"
                     /translation="STAEPALVNAIEQGLRAEQGVVTEDDILMELTKWVEASDNDILS
                     DIYQQTINYVVSGQHPTL"
     gene            293..430
                     /gene="sra"
                     /locus_tag="JNC24_003223"
     CDS             293..430
                     /gene="sra"
                     /locus_tag="JNC24_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary-phase-induced ribosome-associated
                     protein"
                     /protein_id="HAY9159905.1"
                     /translation="MKSNRQARHILGLDHKISNQRKIVTEGDKSSVVNNPTGRKRPAE
                     K"
     gene            587..2284
                     /locus_tag="JNC24_003224"
     CDS             587..2284
                     /locus_tag="JNC24_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HAY9159906.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            2418..3420
                     /gene="adhP"
                     /locus_tag="JNC24_003225"
                     /pseudo
     CDS             2418..3420
                     /gene="adhP"
                     /locus_tag="JNC24_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415995.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
     gene            3566..3850
                     /locus_tag="JNC24_003226"
     CDS             3566..3850
                     /locus_tag="JNC24_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HAY9159907.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVDVSRLRRLVTQ"
     gene            complement(4078..4731)
                     /gene="fdnI"
                     /locus_tag="JNC24_003227"
     CDS             complement(4078..4731)
                     /gene="fdnI"
                     /locus_tag="JNC24_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HAY9159908.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(4724..5608)
                     /gene="fdxH"
                     /locus_tag="JNC24_003228"
     CDS             complement(4724..5608)
                     /gene="fdxH"
                     /locus_tag="JNC24_003228"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit beta"
                     /protein_id="HAY9159909.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEERVAKLKARGYEHAGVYNPKG
                     VGGTHVMYVLHHADQPELYYGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(5621..8668)
                     /gene="fdnG"
                     /locus_tag="JNC24_003229"
     CDS             complement(5621..8668)
                     /gene="fdnG"
                     /locus_tag="JNC24_003229"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(8081..8083),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HAY9159910.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGVLRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETLAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            8900..9781
                     /gene="yddG"
                     /locus_tag="JNC24_003230"
     CDS             8900..9781
                     /gene="yddG"
                     /locus_tag="JNC24_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HAY9159911.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGENGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGACLWIYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYAAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
BASE COUNT         2390 a         2593 c         2504 g         2392 t
ORIGIN      
        1 attcaacagc agaacccgct ctggttaatg ccattgagca aggccttcgg gcagagcaag
       61 gtgttgtcac tgaagatgac attctcatgg agctgaccaa atgggtggaa gcttctgata
      121 acgacatcct cagtgatatc taccagcaaa cgatcaatta tgtggtcagt ggccagcacc
      181 ctacgcttta aggtgctatg cttgatcggc aacctaattt aggggtttag cacgtgtttc
      241 ttcgctacgg cgatgttgtc cttaaaacta gcaacaggat tgaggagtta aaatgaaatc
      301 gaaccgtcag gcacgtcata ttcttggact ggaccataaa atttctaacc agcgcaaaat
      361 agttaccgaa ggtgacaaat ccagcgtggt aaataaccca accggcagaa aacgccccgc
      421 tgaaaagtaa ttcataacca tcagtcctca atgacgatta aacaccattg cctgcgcaat
      481 ggtgtttttg tttttatctg ctttatactt ggggccgacg ccctggcggt aaagcaaaga
      541 cgataaaagc gtgccaggga tggatattca aaaaagagtg agtgacatgg aaccaaaaac
      601 aaaaaaacag cgttcgcttt atatccctta cgctggccct gtactgctgg aatttccgtt
      661 gttgaataaa ggcagtgcct tcagcatgga agaacgccgt aacttcaacc tgctggggtt
      721 actgccggaa gtggtcgaaa ccatcgaaga acaagcggaa cgagcatgga tccagtatca
      781 gggattcaaa accgaaatcg acaaacacat ctacctgcgt aacatccagg acaccaacga
      841 aaccctcttc taccgtctgg taaacaatca tcttgatgag atgatgcctg ttatttatac
      901 cccaaccgtc ggcgcagcct gtgagcgttt ttctgagatc taccgccgtt cacgcggcgt
      961 gtttatctct taccagaacc gccacaatat ggacgatatt ctgcaaaacg taccgaacca
     1021 taatatcaaa gtgattgtgg tgactgacgg tgaacgtatt ctggggcttg gtgaccaggg
     1081 catcggcggg atgggcattc cgatcggtaa actgtcgctc tataccgcct gtggcggcat
     1141 cagcccggcg tatacccttc cggtggtgct ggatgtcgga acgaacaacc aacaactgct
     1201 taacgatccg ctgtatatgg gctggcgtaa tccgcgtatc actgacgacg aatactatga
     1261 attcgttgat gaatttatcc aggctgtgaa acaacgctgg ccagacgtgc tgttgcagtt
     1321 tgaagacttt gctcaaaaaa atgcgatgcc gttacttaac cgctatcgca atgaaatttg
     1381 ttcttttaac gatgacattc agggcactgc ggcggtaaca gtcggcacac tgatcgcagc
     1441 aagccgagcg gcaggtggtc agttaagcga gaaaaaaatc gtcttccttg gcgcaggttc
     1501 agcgggatgc ggcattgccg aaatgatcat cgcccagacc cagcgcgaag gattaagcga
     1561 ggaagcggcg cggcagaaag tctttatggt cgatcgcttt ggcctgctga cggacaagat
     1621 gccgaacctg ctgcctttcc agaccaaact ggtgcagaaa cgcgaaaacc tcagtgactg
     1681 ggataccgac agcgatgtgc tgtcactgct ggatgtggtg cgcaatgtaa aaccagatat
     1741 tctgattggc gtctcaggac agaccgggct gtttacggaa gagatcatcc gtgagatgca
     1801 taaacactgt ccgcgtccga tcgtgatgcc gctgtctaac ccgacgtcac gcgtggaagc
     1861 cacaccgcag gacattatcg cctggaccga aggtaacgcg ctggtcgcca cgggcagccc
     1921 gtttaatcca gtggtatgga aagataaaat ctaccctatc gcccagtgta acaacgcctt
     1981 tattttcccg ggcatcggcc tgggggttat tgcttccggc gcgtcacgta tcaccgatga
     2041 gatgctgatg tcggcaagtg aaacgcttgc acagtattcg ccgctggtgc tgaacggcga
     2101 aggtctggta ctaccggaac taaaagatat tcagaaagtc tcccgtgcaa ttgcgtttgc
     2161 ggttggcaaa atggcgcagc agcaaggcgt ggcggtgaaa acctctgccg aagctttgca
     2221 acaagccatt gacgataatt tctggcaagc cgaataccgc gactaccgcc gtacctccat
     2281 ctaagcctgc gcccggtagt gaaggctacc gggcaatttt cctctccctt tttcagatct
     2341 catccatact gggtagtggc gaataaatct catttgcctc acctgctatg cagaacatca
     2401 tccgaaaagg aggaactatg aaggctgcag ttgttacgaa ggatcatcat gttgacgtta
     2461 cggataaaac actgcgctca ctgaaacatg gcgaagccct gctgaaaatg gagtgttgtg
     2521 gtgtatgtca taccgatctt catgttaaga atggcgattt tggtgacaaa accgacgtaa
     2581 ttctgggcca tgaaggcatc ggtgtggtgg cagaagtggg tccaggtgtc acctcattaa
     2641 aaccaggcga tcgtgccagc gtggcgtggt tctacgaagg atgcggtcat tgcgaatact
     2701 gtaacagtgg taacgaaacg ctctgccgtt cagttaaaaa tgccggatac agcgttgatg
     2761 gcggaagagt gcatcgtggt cgccgattac gcggtaaaag tgccagatgg tctggactcg
     2821 gcggcggcca gcagcattac ctgtgcgggg gtcaccacct acaaagccgt taagctgtca
     2881 aaaattcgtc ccgggcagtg gattgctatc tacggtcttg gcggtctggg taacctcgcc
     2941 ctgcaatacg caaagaatgt ctttaacgcc aaagtgatcg ccattgatgt caatgatgag
     3001 cagttaaaac tggcaaccga aatgggtgca gatttagcga ttaactcacg caccgaagac
     3061 gccgccaaaa ttgtgcagga gaaaaccggt ggcgctcact ctgcggtggt aacagcagta
     3121 gctaaagctg cgtttaactc ggcagttgat gctgtccgtg caggcggtcg tgttgtggct
     3181 gtcggtctgc cgccggagtc tatgagcctg gatatcccac gtcttgtgct ggatggcatt
     3241 gaagtggtcg gttcgctggt cggcacgcgc caggatctaa ccgaagcctt ccagtttgcc
     3301 gccgaaggca aagtggtgcc gaaagtcgcc ctgcgtccgt tagcggacat caacaccatc
     3361 tttaccgaga tggaagaagg caaaatccgt ggccgtatgg tgattgattt ccgtcactaa
     3421 gaggtctttg ctgcgactcc catgttcggg tcgcagcatc gcacactctc caacatgaaa
     3481 tggctaaatg gattattgtt gcgtaggaag gaacaataat gatagtgttc ccagagaagg
     3541 aacgctactg agagtaaaga cactaatgaa aatggccaat catccccgcc cgggggacat
     3601 tattcaggaa tcactggacg aacttaatgt cagcctgcgc gagtttgcca gagcaatgga
     3661 aattgcgccc tcaacggcaa gccgattgct gaccggaaaa gcagctttga cgccagaaat
     3721 ggcaattaaa ctttccgtgg tgatcggcag ttcgccgcaa atgtggctga atctgcaaaa
     3781 cgcctggagt ctggcagagg cagaaaaaac ggtggatgtg tcgcgattac gccgtctggt
     3841 tacgcaataa aaaatggcac cttataaagc gccatttgaa actgatgaca aacgcaacat
     3901 tgcctgatgc gctacgctta tcaggcctac acgttcactt caatctattg tatttgaagg
     3961 gttttcatgc cggataaggc attcacgacg catccgggat gaacaaagcc cacgttgtct
     4021 acaatatgaa aatggcgcct tttacagcgc cattttcagc ttaaagttca aaagagatta
     4081 tatcccttct tcactctctt ttttcgcttc tgccttctcg atttcacgat accagcgcgg
     4141 atggtgtttc ttcgcccagc gacggctcac cttcccttcg atcatccctt taatcgaccc
     4201 tttcacccag aatgccatat acatatggat caggatggcg tggatcagga tgatacccgc
     4261 agccgcgtgg atcagcaggc tgtagcgaac aacctgcatc gggaagtact gcgcaaagta
     4321 cggacgccag ataatcaccc cggtcaccag cagcacgaaa atcatgctca tgatcgacca
     4381 gaacatcatc ttttgcccgg cgttgtactt accaacatcc gccactttgt gctcattgcc
     4441 ttttaatact tcgacaatgt tcaacagcca cggaatatct ttcttatccg ggatgttgtg
     4501 atgcacaaaa cgcacaaaca taaacatcag tgcgacgaaa atcgcaatgc cgaagaacgg
     4561 gtgcaaaatg cgtcccatct gcggcgtacc gaaggtttgc gtcagccatt gcagcgtcgg
     4621 gaagaagaac gaaatcccgg acagcgccac caggaagaag caaatcacca cggtccagtg
     4681 acaggcgcga tcaataaatt tggtgcgcac aatcattttc gacttactca tgatgatcct
     4741 cctcgtcatc gtccacttcc ttattcgggc caataccgat gtagtggaaa atcaacccgg
     4801 caaaagtggc aataaagcca gccgctgcca gcggtttcaa cgcgcctttc cacaggctta
     4861 ccgaggtgtc gatcttcgga tctttcggca gaccgtaata cagctccggc tgatcggcgt
     4921 gatgcagcac gtacataacg tgcgtaccac cgaccccttt cgggttgtag acgccagcat
     4981 gttcgtaacc acgcgctttc agtttcgcca cgcgctcttc cgccagctcc agcatctcct
     5041 tcttggtgcc gaagtggata gccccggtcg gacaggtttt cacacaagcc ggttcctggc
     5101 cgacgctgac gcgatcgacg cagagcgtgc atttatatac ccggttatcc tctttgttga
     5161 ggcgcggaat attaaacgga cacccggcaa tgcagtaacc acagccgatg cagttttccg
     5221 actggaaatc gacaatcccg ttagcgtact gaatgattgc accagcagac gggcacgcct
     5281 tcaggcagcc gggatcttca cagtgcatac agccgtcttt acggatcagc cactccagct
     5341 tgccgttctg ttcggtttcg ctaaagcgca tcaccgtcca ggacttggcg ctcagatcgg
     5401 cggggttatc gtaaaccccg acgcagtgcc ccacttcatc acggatgtcg ttccactccg
     5461 aacacgccac ctgacaggct ttacagccga tacaggtgga aacgtcgata agttttgcga
     5521 cttctgcttt gtaatcacgc acctgagaag gcggcgtgat ggagttagtt gcggaccttt
     5581 tgataatatc ctgcgtttcc atagccatct gttcgccccc ttacgccttc tcgatgttga
     5641 ctaagaacgc tttatattcc ggcgtttgcg agtttgcatc accgacattc ggcgtcagag
     5701 tgttagcgat ataaccttta cgcgcgacac cctcaaagcc ccagtggatt ggaataccca
     5761 ccgtttcaac ctgctgaccg tttacattca gcggtttcag acggcgcgtt accaccgcca
     5821 ccgcgcggat aaagccacgc ttgcttgaga cagtgacacg atcgccatta ttaatgcctt
     5881 tcgccgccgc cagcgtttcg ctgatttcca caaactgttc cggctgagca attgcgttga
     5941 gcaatgcgtg cttggtccag gtgtggaagt gctcggtcag acgataggtc gtacccacat
     6001 acgggaactg ctctttttta cccatccgca gcgcgtcttg ttcatacaga cgaacaaccg
     6061 ggttagacac cacgttcgga tgcagcgggt tagtgcccag cggcgtttca atcggctcgt
     6121 agtgttccgg gaacggacct tccgccattt tgttgatggc aaacaggcgt cccatccctt
     6181 ccggctgcat gataaacggc ccggttggcg tacccggtgc ggcattgccg aagtccggaa
     6241 tatcgttacc cgtccacttg ctgccgttcc actggatcag catccgtttc ggatcccacg
     6301 gtttaccatt gatatccgcc gaagcacggt tgtagagcac gcgacggttg agcggccacg
     6361 cccaggccca tcccagcgta ttccccagac cggatgggtc ggagttatcg cggttagcca
     6421 tctggttgcc ctgctctgtc cagctaccgg tgtagatcca gcaagaagat gcggttgtac
     6481 cgtcatcacg cagatgcgca aagctactca gcaactgacc tttcttcgca atcagcacgc
     6541 cattagcgtc atagagatct tccagcgcat agccgttatt ctctttcgcc acttcgtcag
     6601 attgcggttc gtgcggctgc ttgtagttcc agctcatctt catcagcggt tctacgcctt
     6661 taccaccttc ggcctggtac agctcgcgca gatgatggta gatacccgcc agaatttcgc
     6721 cgtcgttacg cgcttcgccc ggcgcgtcct gacctttcca gtgccactgc aaccagcgac
     6781 cggagttagc gatagaacca tcttcttcgg caaagcaggt cgaaggcaga cggaatactt
     6841 cagtctgaat agacgccgga tcgacatcgt tcgactcgcc gtggttctgc cagaaggtag
     6901 aggtttcagt caccagcgga tcgataacca ccatgtactt cagcttgctc aggcagctca
     6961 ccactttgtt tttgtccggg aaggacgcaa ccgggttaaa gccctggcag aagtaaccgg
     7021 tgactttgcc ttcgtccatc atgttgaaat acttgatgac gtcgtaggtc tggtcccact
     7081 tcggcagcca gtcatagccc cagttgttct ctttctgcgc ggcatcgcca tagaaagatt
     7141 tcatcaggct aacgaagaac ttcggatagt tgctccagta gttcacctga tcagccagcg
     7201 tcgctttcgg cgtgttcgct tccagatacg actgcaaatc aacctgtttt tctgacggaa
     7261 gcgtcagata acctggcagg ctggtagaga gcagacctaa gtcagtcagc ccctgaatgt
     7321 tggagtgacc acgcaatgcg ttcacgccgc caccggccat acccatgtta ccgagcagca
     7381 actggatcat cgccatagta cggatgttct gcgcacccac ggtgtgctgc gtccagccca
     7441 gcgcgtacag gaaggtggtt gtgcgatccg gtgcgctggt ggaggccagc acttcacaca
     7501 ctttcaggaa gtcggctttt ggcgtaccgc agatgttttc aacgacgtcc ggcgtgtagc
     7561 gggaaacgtg cgctttcagc aggttccaca cacagcgcgg atgagtcagt gtttcatcgc
     7621 gttttgcata gccgttttca tcgaactgat agttccagga cgacttatca tactggcgtt
     7681 tttcagcgtc gtagccgctg aacaggccgt cttcgaaagc aaaatcatca cgcaccagca
     7741 ggctggcgtt ggtgtaatgt ttaacgtatt cggcgttgat tttgttgttt tcgatcaggt
     7801 agcgcaaaac gccagacagg aacgtaatgt ccgtaccgga acgaataggt gcgtaaatat
     7861 ccgccacaga agcggtacgc gtaaaacgag gatcgacaac gatcaaggtt gcatcgttgt
     7921 tgtttttcgc ttccatcgcc cagcggaaac cgacgggatg cgcttcagca gcgttaccgc
     7981 ccattaccat tacgacgtta gcgtttttga tatccaccca gtggttggtc atcgcaccgc
     8041 gaccaaatgt tggagcaaga cttgctaccg ttggtccgtg tcagacgcgc gcctggttgt
     8101 ctaccgccag catcccgagg gagcgggcaa atttctgggt cagcatcccg gtttcgttgc
     8161 tggcaccgga ggcacacagc ataccggtag aaagccaacg gtttaccgtt acgccctgct
     8221 cgttcttttc aataaagtta gcgtcacggt cagctttcat cagcttcgca atacgggaga
     8281 atgcttcttc ccagctaatg cgctgccatt tgtcagaacc tggcgcacga tattccgggt
     8341 agcgcagacg gttttcgctg ttgacgtaat ccagcaaacc ggcccctttt gggcacagcg
     8401 caccacggct taccggatga tccgggtcac cttcaatgtg ataaatcgct tctctggcgt
     8461 ttttcgcgcc atcacccagg ctatacatca atagcccgca acctacggaa cagtatgtgc
     8521 aggtgttacg gatctcttta gcgcgtaata atttgtagtt tcgcgcctga gccagtgctt
     8581 gcttcggggc aaagcccaat gccgctaccg ttgttccagc cataccgccc gcgcagattt
     8641 taaaaaattg tctgcgactg acgtccattg ctttcctctt ttttcagggg tattactgcg
     8701 cgaggaaact aacagtaaaa ttctgaatct ttttgcgcga aatcaaaaag aacacgtgta
     8761 tgccacaaaa taatccgcct gcgcaaatta acctacctca atagcggtag aaaaacgcac
     8821 cactgcctga caggccagtt aaaaaaatgc tataaaattc agcttaattt ttaacggcaa
     8881 gagagacaaa acagcgagca tgacacgaca aaaagcaacg ctcatagggc taatagcgat
     8941 cgtcctgtgg agcacgatgg taggattgat tcgcggtgtc agcgaggggc tcggtccggt
     9001 cggcggcgca gctgctatct attcattaag cgggctgctg ttaatcttca cggttggatt
     9061 tccgcgtatt cggcagattc cgaaaggcta tttactcgcc gggagtctgt tattcgtcag
     9121 ctatgaaatc tgtctggcgc tttccttagg gtatgcggcg acccgtcatc aggcgattga
     9181 agtgggtatg gtgaactatc tgtggcccag cctgacaatt ctctttgcca ttctgtttaa
     9241 tggtcagaaa accaactggc tgattgtacc aggattatta ttagccctgg tcggcgtctg
     9301 ctgggtgtta ggcggtgaga atgggttaca ttatgatgaa atcatcaata atatcaccac
     9361 cagcccattg agttatttcc tggcgtttat tggtgcgttt atctgggcag cctattgcac
     9421 agtaacgaat aaatacgcac gcggatttaa cggaattacc gtttttgtcc tgctaacggg
     9481 agcatgtctg tggatttact attttctcac gccacaacca gaaatggtat ttagcacgcc
     9541 cgtcatgatt aaactcatct ctgcggcatt taccttagga tttgcttatg ctgcatggaa
     9601 tgtcggtata ttgcatggca atgtcaccat aatggcggta ggctcgtatt ttacgcctgt
     9661 actttcctca gcgcttgcag ccgtgctgct cagcgccccg ctgtcgttct cgttctggca
     9721 aggcgcgcta atggtctgcg gcggttccct gctctgctgg ctggcgacac gtcgtggtta
     9781 aaataattgc cgggtctacg gcccggcagt taatcagata aattccgaca atattaaaaa
     9841 ataaatttca aataccagta attcacctta tatctcatt
//
LOCUS       DADMKF010000139         9855 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.142, whole genome shotgun sequence.
ACCESSION   DADMKF010000139 DADMKF010000000
VERSION     DADMKF010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9855
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.142"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(<1..159)
                     /locus_tag="JNC24_003231"
     CDS             complement(<1..159)
                     /locus_tag="JNC24_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9159912.1"
                     /translation="MLQSSADKACNVLVKKVRYLKDVREITKMMNGKLIYSWLKWQCG
                     EFFSLNYKL"
     gene            237..1331
                     /gene="ddlA"
                     /locus_tag="JNC24_003232"
     CDS             237..1331
                     /gene="ddlA"
                     /locus_tag="JNC24_003232"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HAY9159913.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALKRHAADNALKATM"
     gene            complement(1355..1567)
                     /locus_tag="JNC24_003233"
     CDS             complement(1355..1567)
                     /locus_tag="JNC24_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HAY9159914.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGLLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            1827..2135
                     /locus_tag="JNC24_003234"
     CDS             1827..2135
                     /locus_tag="JNC24_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2755 family protein"
                     /protein_id="HAY9159915.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLVGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(2194..3288)
                     /gene="yaiW"
                     /locus_tag="JNC24_003235"
     CDS             complement(2194..3288)
                     /gene="yaiW"
                     /locus_tag="JNC24_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HAY9159916.1"
                     /translation="MSRVNHLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLAITFESQGLAPTLENVCSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(3301..4521)
                     /gene="sbmA"
                     /locus_tag="JNC24_003236"
     CDS             complement(3301..4521)
                     /gene="sbmA"
                     /locus_tag="JNC24_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HAY9159917.1"
                     /translation="MFKSFFPKPGAFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFAFFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGWLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            4873..6030
                     /locus_tag="JNC24_003237"
     CDS             4873..6030
                     /locus_tag="JNC24_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OXA-12 family class D beta-lactamase AmpH"
                     /protein_id="HAY9159918.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITRPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPGIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(6031..6654)
                     /gene="iprA"
                     /locus_tag="JNC24_003238"
     CDS             complement(6031..6654)
                     /gene="iprA"
                     /locus_tag="JNC24_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide resistance inhibitor IprA"
                     /protein_id="HAY9159919.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNNEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     ITLIEQNQLWRDAFYWLAWQNRILELRDVQLIGHNSYEQIRATLLSMIDWNEELRSRI
                     GVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAINRLPSEY"
     gene            complement(6742..9630)
                     /locus_tag="JNC24_003239"
     CDS             complement(6742..9630)
                     /locus_tag="JNC24_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HAY9159920.1"
                     /translation="MHSWKKKLVVSQLALACTLAITSQANAATYNTFGYHDDAATIYN
                     WGDSTKIDHDYLTYGGYVYDHAADGYFDTTFSGDTVNGVISTYYLNHDYGTDTGNTLN
                     ISNSIVHGMVTSYQVGYGDYVWTNGSDYTGHDWVDGDIFTLNIANSTIDDDFDAFYFT
                     DTYLDADGKTSKTDYDRLVTAGLGTAVTLDVESNINISNNSHVAGITLVQHDLGNATY
                     NTEGHQWDNNIVVNDSTVTSGSWTEDEQSDRGHFGNSVEPSDYGNGASPADDVALAFI
                     DDDTSDYRMVNNVTFNNSQLLGDVVFDSTWNANFDATGHLIDNSTTAYTHGGWATDDQ
                     NVDHLNLTLNNTKWVGSANIDYDVVVADEAFYDVAPNSLNPYASYSEDGWNRVDNANA
                     FQSGVFDVVLNNGSDWETTKTSLIDTLAINSGSQVNVSADSSLTSDTITLNGGSSMEV
                     NGEVNTDHLTIDTFSTVNFGEDTASAWAYAPLYANTITVTNGGVLDVNTNMNDISSVF
                     ATDTLELTSGNVKDNNGNVYAGVFDIHSNDYILNADLVNDRTNDTSKANYGYGVIAMN
                     SDGHLTVNGNNDINNGDEVDNSSADNVVAATGNYKVRIDNSTGAGAIADYAGKQLIYI
                     DDTKTNATFSAANKADLGAYTYQAEQRGNTVVLQQMELTDYANMALSIPSANTNIWNL
                     EQDTVGTRLTNSRHGLADNGGAWVSYFGGNFNGDNGTINYDQDVNGIMVGVDTKVDGN
                     NAKWIVGAAAGFAKGDMNDRSGQVDQDSQTAYIYSSAHFANNVFVDGSLSYSHFNNDL
                     SATMSNGTYVDGSTNSDAWGFGLKAGYDFKLGDAGYVTPYGSVSGLFQSGDDYQLSND
                     MKVDGQSYDSMRYELGVDAGYTFTYSEDQALTPYFKLAYVYDDSNNDNDVNGDSIDNG
                     TEGSAVRVGLGTQFSFTKNFSAYTDANYLGGGDVDQDWSANVGVKYTW"
BASE COUNT         2548 a         2424 c         2363 g         2520 t
ORIGIN      
        1 taacttataa ttaagagaaa aaaactcacc gcattgccat ttaagccagg aataaattag
       61 cttcccgttc atcatttttg tgatctcgcg cacatctttg agataacgca ctttcttcac
      121 cagtacgttg cacgctttgt cagcagaaga ttgcaacaat ctttcagaag gccgcgaatc
      181 ccccgttttg tcgtggtaat gtatgcactt tgtgttagac gggataggtt tttaagatgg
      241 aaaaactgcg ggtaggaatc gtttttggtg gtaaatcagc ggaacatgaa gtgtctctgc
      301 aatcggcaaa aaacattgtc gatgccattg ataaaagtcg cttcgacgtt gtgctgctgg
      361 gcattgataa acaagggcaa tggcacgtca gcgatgccag caattatctg ctaaatgcag
      421 acgatcctgc ccatattgcg ttgcgccctt cggcgaccag ccttgcgcag gtgccaggta
      481 aacatgagca tcagcttatc gacgcgcaaa acggtcagcc gttgccgacg gtggatgtca
      541 ttttcccgat tgtccacggt acgctgggcg aagatggttc cttgcaggga atgctgcggg
      601 tcgccaattt accgtttgta ggttctgatg ttctggcttc agcagcctgt atggataaag
      661 atgtcaccaa acgtctgcta cgcgatgccg ggctgaacat tgcgccattt attaccctga
      721 cgcgcgctaa tcgtcacaac atcagttttg ccgaagtgga gtctaaactg gggttaccac
      781 tgtttgtaaa accggctaat cagggctctt ctgttggtgt cagcaaagta accagtgaag
      841 aacagtacgc aattgccgtc gatctggcgt tcgagttcga tcataaagtg atcgttgagc
      901 aagggatcaa aggtcgtgag atcgaatgcg cagttctggg caacgacaat ccgcaagcca
      961 gcacctgtgg cgagatcgta ctcaccagcg atttctatgc ctacgacacc aagtacattg
     1021 acgaagatgg cgcgaaagtg gtagttccgg cagccattgc accagagatc aacgataaga
     1081 tccgggcgat tgccgttcag gcttatcaga cgttgggatg cgcaggcatg gcgcgtgtag
     1141 acgtgttttt aaccccagag aacgaagtgg tgatcaacga gatcaacaca ctgcctggct
     1201 tcaccaacat cagtatgtat ccgaagctgt ggcaagccag cggtctgggt tacaccgatc
     1261 tgatcacacg tttgattgag ctggcgctga agcgtcacgc tgcggataac gcattgaaag
     1321 ccacaatgta atattttaac gcccgataat attcttattg ggcgttttca gtttcttcat
     1381 cgcgacgacg aataatcaac cccgccagcc agaaactaat cacccacgtc accagaccga
     1441 cggcataggt ctgccaacct tttgcttcaa atcccagtaa ccccaccacg ccattaagaa
     1501 tgaatataag gccaatggcg aacgcatagt agtgccagtc acggcggatt tttacaggca
     1561 gattcatggc agctccgtta acgaaaaaca acatccttgc atattctgta acaaccgtct
     1621 gtggtgtaga caacattttc gtaattgtta ataaatttaa cttaaggttg ccattttgta
     1681 actcacaacg aagattcacg cttcgggaat attcccaaca tcaatttacc atgattctga
     1741 tattgacaat ctaaatgacc tgtcagactt gccataagcc gctggtatca caccagaaga
     1801 gaagcagatg acttccggag gtttttatgg ctgatttcac cctgtcaaaa tcgctgttta
     1861 gcggaaaata tcgcaatgcc tcttcaacgc ctggcaacat tgcctatgcg ttgtttgtgc
     1921 tgttttgctt ttgggctggg gcgcaattgc tgaacctgtt agtgcatgcg cccggcgtct
     1981 atgagcgttt aatgcaggtc caggaaacag gtcgcccacg ggtggaaatt ggtttaggtg
     2041 tcggcaccat tttcgggctg atcccgtttt tagtaggctg cctcattttt gcagtggtgg
     2101 cgctatggct gcactggcga catcgccgcc agtaagattt acgttgccag atacggccta
     2161 aaacgcctta tctggcatga cattcctgtt tgatcactgt ttcatacaac gcgcccgccg
     2221 ttcgtctacg cgcttcgcaa accaggccgt agtcaggttg cgggtgattt tcgggctttc
     2281 cagttgaatg ccaggcaaca tttcgcgggg tagtgattta cccgttttcg cttcggcaag
     2341 ttgataaact ttcttgtaca gcgccgtctc ctcaaaagag aagctgtcac ctttctctaa
     2401 ctgacggcga atttcgctgt cgctcatccc cagttttccc gccagtttgc gcgttgccag
     2461 ttcggttttc ccgggttctt tactgtcgta acgaattaaa tcaccgtcca gtgccagctt
     2521 cacgccgctg gccttactga ctgcgttctg aaacgcggca ttacggctgg cgtaccagcc
     2581 agcgttaaaa tcagcaaagc ggtatatcgg tgcgctatag ctggcgggat aattcagtaa
     2641 atggtaagta ccaaaccaca acccgccgcg gcgactgaag acttcctgac gaactgtacc
     2701 gtccattttc cacggatacc ctttggtatg ctgttcggca aaagcaatgc tgacctgcat
     2761 cggcccaccg gtgcgcaccg gattaagcga accaaacagc gtctgcccca ttggtaccat
     2821 gctgatcaga tcgtcaaaaa tcgcgctcaa ttgcttttct gtacgcaccg aatctaaccg
     2881 ttcgctataa cttttgccgt ttggcgattt gatttttagc gcggtatgaa ccagaaaagc
     2941 aggaatgtgc atccgttcag cacgacggtc aatctcttgc caggcgatct tgcttaagcc
     3001 cggaacggcc ggatctgcct gataatttga ctcctgctgc gccaccgcca gcaccgagca
     3061 gacgttttcc agcgtcggtg ccagcccctg actttcaaag gtgatggcga gatctttcgc
     3121 ccaggcatcg cggtctttca cgctcgcagg catcttctgc cgcacgacgc tcgctacatc
     3181 tatcgctttt tcgccctttt tcaacggttg cggagcctga ctactacacc ccgctaaaac
     3241 cagaaccgcc aacaatgaca gcgaagatag atgattgacg cgcgacatta cttctccttt
     3301 ttagctcaag gtatgggtta cttcctgaat tttgtcacca tccagctcat gttcaaagct
     3361 gcgcagacgt ttgtagatag acatcaactc aaccagtgtg gtccatgagt taatcaggta
     3421 ctggaaagca ccgcgaacct gaccaaaaac gttggtaatc tgcgtcatca ggccgagcgt
     3481 aatcgtaccg gcaacaattg acggaaacag caagaacaaa ccgaaaacgt tatcgacctg
     3541 caaatagaga atgcgggcga tgttgaaata catatagtga aaatagaggc gaaaatagtt
     3601 tttccgtacg gcgctaaaca gctcgcgtac cgtaggcggc gtcgcgcgcg tggcatcgtc
     3661 ttcaccataa accagctctt tacggtaggc ggcctctact cgctggtttt taaactccag
     3721 ccccggcagt ttgatcccta ccactgccag ccatccggtc cccatcagcg accagacgat
     3781 agcggcaatc accagaccat acggaatgtg cccgataatc ggcagctccg gcacatgcgc
     3841 ggagagcgtt accagcaccg gcaggaaggc gatcaacgtc atgatggcgt tgataaaact
     3901 gacgcccata ttctccagcg ttgaagcaaa acgcatggtg tcttcctgca cacgctgtgc
     3961 ggcaccttcg atatgacgca gttgttgcca gttcgccatg taatattcgt tcatcgccgt
     4021 acgccagcgg aacacatagt gactgacaaa gaagttgttc agtacactga tcaccaccgc
     4081 gatcagcgca atccccagaa agacgcccac ttcgcggtaa aattgttcga tagtgacttt
     4141 atgcggcgaa cttagcgcgg tttgaatcag atcatagaac ggtgcatacc aggcgttgac
     4201 ggcgaccccg acttccacca aaaaccaggt gacgaagatg atcagtgcag taccgagtat
     4261 tgaccagtat tgccaacggt gcgggctgta gataaaccag aaaaatgcaa aaagtcctac
     4321 gcaaacaatg tagtaagcgt aaaaaatcag gaaatccaac gaccagaaac gcgcggcgct
     4381 aatcgggatc tgcccggaag cgccggtgat acgcgccacc cagtctcccc caccggcttg
     4441 ccagaagata acggcaatca atgcccaaac aaaggccgag agaaaaaacg ctcccggctt
     4501 tgggaaaaaa gacttaaaca tatgcactcc tgctaacttc ttatcgtttt aacaactgct
     4561 gtgtattaac cgcatgacca ctacaaagcg tcagccaaat gaaaccgccc attgtgaccc
     4621 atagcaaggc gcttagttcg ccagcctgac cataagaatt gtttcgacgg atttcacaaa
     4681 ccaaagattg tttacataag cggattaaca cgcgcaggat cacaaatgca ggcattggtg
     4741 agcaggaaaa agatcgtgtt tacgcgccgt tacgttttta acactattat tgcactaatt
     4801 cgccctttgc aatccatcaa tgagtagtat aaatacgctc agttaccttc attcaatcta
     4861 tggacaccac cgttgaaacg tagtctgctt ttttctgccg tgctgtgtgc ggcgtcattg
     4921 acctccgtcc atgcggcgca gccgatcact gaaccggagt ttgcctctga tattgtcgat
     4981 cgttatgccg atcatatttt ttacggcagc ggtgcaacgg ggatggcgct ggttgttatc
     5041 gacggtaacc agcgcgtctt tcgtagttat ggcgaaacgc gacctggtaa taacgttcgc
     5101 ccgcagctgg attcggtcgt gcgtattgct tccctcacca aactaatgac cagtgaaatg
     5161 ctggtgaaat tgctcgacca ggggaccgta aagttaaacg atccgctaag taaatatgct
     5221 ccgccaggcg cacgcgtgcc gacctacaat gggacgccca ttaccctagt gaatctggca
     5281 acccatacca gcgccctgcc ccgtgaacag cccggtggcg cggcacatcg tccggtgttt
     5341 gtctggccaa cgcgcgagca acgctggaaa tacctttcta cggcgaagct gaaagccgcg
     5401 ccaggcagtc aggcggcgta ctctaacctt gcgttcgacc tgctggcaga tgcactggcg
     5461 aatgcgtccg gcaagcctta tacccagttg tttgaagagc agattacccg tccattgggg
     5521 atgaaagaca ccacctacac cccctcaccg gatcagtgcc gccgtttaat ggttgctgag
     5581 cgcggtgcca gtccgtgcaa taacacgctg gcggcaattg gcagcggcgg cgtctattcc
     5641 acgcctggcg atatgatgcg ctggatgcag cagtatttgt catctgattt ttatcagcgc
     5701 agtaaccagg cagatcgcat gcagacgttg atttatcagc gcgcgcagtt tacgaaagtg
     5761 attggcatgg atgtccccgg caaagcggat gcgcttggtt taggctgggt atatatggcc
     5821 ccgaaagagg gtcgtccggg gattattcag aagacaggcg gtggtggcgg tttcattacc
     5881 tatatggcga tgatcccgca gaagaatatt ggcgcgtttg tggtggtcac tcgttcgccg
     5941 ttgacgcgct ttaaaaatat gagcgatggc atcaatgatc tggtgaccga gctaagcggg
     6001 aataaaccgt tggttatccc cgcatcctga ttaatactct gaaggcaaac ggttgatagc
     6061 gaccagtttg cctttattca tttcgatata gccgccttta cgcaaagcag cgagaacttc
     6121 tgcgacgaca gaacgcgata tgcgtgtacg ttgatggata taattcatca caccaatacg
     6181 tgatcgcaat tcttcattcc agtcaatcat tgataataat gttgcgcgga tttgttcgta
     6241 ggaattatgc ccaatgagct gcacgtcgcg taattccaga attctatttt gccaagctaa
     6301 ccagtaaaaa gcgtctcgcc agagttgatt ttgttcaata agcgtaatgg tttgtttggc
     6361 tggtagatga tatcccgtac aatttccttc tgatattaat ttgtatggta tatcgttttt
     6421 cattaaacca tcagccagcc ccataatata aggggcctgg gtaataccga taagtacgtt
     6481 ttcttctcta cgcagagaga taactccctc taaaataacg aaagtatctt cgttattgac
     6541 atcactggaa aatataactt gcttttcatt attaaactcg aagcgcgtac cgtatctgga
     6601 caaacattta tcgagcttac caaattcctg aagaggttta actacagata acatttgcgc
     6661 gtcctttgca gtaatgcccg tcaaatcctt gacgggcatt atttagatta aattaccagt
     6721 atttcttcgg agtgaagaat attaccaggt atatttaaca cccacgttcg cggaccagtc
     6781 ttgatctacg tcaccaccac cgaggtagtt agcatcggta taggcgctga agttcttggt
     6841 gaagctgaac tgagtaccca gaccaacacg taccgcagac ccttcagtac cgttatcgat
     6901 ggaatcaccg ttcacatcgt tatcgttgtt agagtcgtcg tagacgtaag ccagtttgaa
     6961 gtacggagtc agagcctgat cttcgctgta ggtgaaggta taacctgcat ctacacccag
     7021 ttcataacgc atgctgtcgt aagactgacc gtcaactttc atgtcgttgc tcagctggta
     7081 gtcatcacca gactggaaca gaccagaaac gctgccgtaa ggagtcacgt aaccagcatc
     7141 acccagtttg aagtcgtaac cggctttcaa gccgaagccc caagcgtcgg agttggtgct
     7201 accgtcaacg taagtaccgt tgctcatggt tgcagacagg tcgttgttga agtgagagta
     7261 gctcaagcta ccatcaacaa agacgttgtt cgcgaagtga gcagaagagt agatgtaggc
     7321 agtctggctg tcttgatcta cctgaccaga acggtcattc atgtcacctt tagcgaagcc
     7381 tgcagccgca ccgacgatcc acttagcgtt gttaccgtca actttggtat caacaccgac
     7441 catgatgccg ttaacatcct gatcatagtt gatggtgccg ttgtcgccgt tgaagttacc
     7501 accgaagtag cttacccatg cgccgccgtt atcagccagg ccatgacgag agttggtcag
     7561 acgagtacca acggtgtctt gttccaggtt ccagatattg gtgttcgcag acgggatgct
     7621 cagcgccatg ttagcgtagt cggtcagctc catctgttgc agaacaacgg tgttaccgcg
     7681 ctgttcagcc tgataggtgt atgcacccag gtcagctttg ttagcagcag agaaggtcgc
     7741 gttggttttg gtatcatcga tatagatgag ctgcttacct gcgtaatcag caatcgcacc
     7801 agcgccagtg gagttgtcga tacgaacttt gtagttaccg gttgcagcta caacgttgtc
     7861 tgcagagctg ttgtcaactt cgtcgccatt gttaatgtcg ttgttaccat taacggtcaa
     7921 gtgaccatca gagttcatcg caataacacc ataaccgtag ttagctttgc tggtgtcgtt
     7981 ggtacgatcg ttgacgaggt ctgcattcag gatgtaatca ttactatgaa tgtcaaacac
     8041 acctgcgtaa acattaccgt tgttatcttt aacgttgcca ctggtcagtt ccagagtatc
     8101 agttgcaaat acgctggaga tgtcattcat gttggtgttt acatccagca caccaccgtt
     8161 agtaacggta atggtattgg catacagcgg tgcatatgcc caagctgaag cagtatcttc
     8221 accgaagtta acggttgaga aggtatcaat ggtcaggtga tcggtgttaa cttcaccgtt
     8281 aacttccatt gaagaaccac cgttcagagt gatggtgtca gaagtcagag aagaatctgc
     8341 actcacgtta acctgagaac cgctgttgat cgccagggta tcgatcaggg aagttttcgt
     8401 ggtttcccaa tcagaaccgt tgttcagaac aacatcgaat acgccgctct ggaatgcgtt
     8461 agcgttatca acacgattcc agccatcttc agagtaagaa gcgtacgggt tcaggctgtt
     8521 tggtgcaacg tcgtagaagg cttcgtcagc aacaacaacg tcataatcaa tattcgctga
     8581 accaacccat ttggtgttgt tcagagtcag gttcaggtga tcgacgttct gatcgtcagt
     8641 tgcccaaccg ccatgagtgt aagcagtggt agagttgtca atcaggtgac ccgtcgcgtc
     8701 aaagttagcg ttccaggtac tgtcaaatac aacgtcacca agcaactgag agttgttgaa
     8761 cgtaacgttg ttgaccatac gataatcaga agtgtcatca tcgatgaagg ctaacgcaac
     8821 atcatcagca ggagatgcgc cattgccgta gtcacttggt tcaacagaat tgccgaaatg
     8881 accacgatct gattgttcgt cttcagtcca tgaaccagaa gttacagtag agtcattaac
     8941 aacgatgttg ttgtcccact gatgaccttc ggtattgtag gtcgcattac cgaggtcgtg
     9001 ttgaaccaaa gtaatacctg caacatggga gttgttgctg atgttgatgt tactttcaac
     9061 atccagagtc acagcggtac caagaccagc ggttaccaaa cgatcataat ctgtcttaga
     9121 tgttttaccg tctgcatcca gataagtatc ggtgaagtag aaggcgtcaa aatcatcgtc
     9181 aatagtagaa ttagcgatgt tcagagtgaa gatgtcgcca tcaacccagt cgtggccagt
     9241 ataatcgcta ccgttggtcc agacgtagtc gccataacca acctggtaag atgtaaccat
     9301 accatgaaca attgagttgg agatgttcag tgtattaccg gtatctgtgc cataatcgtg
     9361 gttcagataa taagtagaaa ttacaccatt tacagtgtcg ccgctgaaag tggtatcaaa
     9421 gtaaccatca gctgcgtggt catatacata accaccgtaa gtcagataat cgtgatcaat
     9481 tttagtgctg tcaccccaat tgtagatggt ggctgcgtca tcgtgatacc cgaaggtgtt
     9541 ataagtagct gcattagcct gagaggtgat agccagagtg caagccaatg ctaattgtga
     9601 tactacaagt ttctttttcc aggagtgcat ttgtcatccc tcctcaggga cgtaataagt
     9661 tgatccatca attatcaatg catagaaaac atattatgtt tcgcgctaac gattatgctg
     9721 cgttacagac gaatggttca attattcttt cccctaagtc cgaatgtgga catatataat
     9781 ttatggagaa aaacacgcat caaattgata aaaggacgat atttcaacat gttgcagata
     9841 atgttatttt tgata
//
LOCUS       DADMKF010000140         9851 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.143, whole genome shotgun sequence.
ACCESSION   DADMKF010000140 DADMKF010000000
VERSION     DADMKF010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9851
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.143"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(382..1359)
                     /gene="epmA"
                     /locus_tag="JNC24_003240"
     CDS             complement(382..1359)
                     /gene="epmA"
                     /locus_tag="JNC24_003240"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HAY9159921.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            1684..3492
                     /gene="frdA"
                     /locus_tag="JNC24_003241"
     CDS             1684..3492
                     /gene="frdA"
                     /locus_tag="JNC24_003241"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HAY9159922.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQECFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            3485..4219
                     /locus_tag="JNC24_003242"
     CDS             3485..4219
                     /locus_tag="JNC24_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HAY9159923.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALVYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            4230..4625
                     /gene="frdC"
                     /locus_tag="JNC24_003243"
     CDS             4230..4625
                     /gene="frdC"
                     /locus_tag="JNC24_003243"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HAY9159924.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            4636..4995
                     /gene="frdD"
                     /locus_tag="JNC24_003244"
     CDS             4636..4995
                     /gene="frdD"
                     /locus_tag="JNC24_003244"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HAY9159925.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
     gene            5058..6191
                     /gene="blaEC"
                     /locus_tag="JNC24_003245"
     CDS             5058..6191
                     /gene="blaEC"
                     /locus_tag="JNC24_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HAY9159926.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLTPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            6280..6813
                     /locus_tag="JNC24_003246"
     CDS             6280..6813
                     /locus_tag="JNC24_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HAY9159927.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDREMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(6810..7127)
                     /locus_tag="JNC24_003247"
     CDS             complement(6810..7127)
                     /locus_tag="JNC24_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound-resistance protein
                     SugE"
                     /protein_id="HAY9159928.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(7302..7448)
                     /gene="ecnB"
                     /locus_tag="JNC24_003248"
     CDS             complement(7302..7448)
                     /gene="ecnB"
                     /locus_tag="JNC24_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HAY9159929.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(7559..7684)
                     /gene="ecnA"
                     /locus_tag="JNC24_003249"
     CDS             complement(7559..7684)
                     /gene="ecnA"
                     /locus_tag="JNC24_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HAY9159930.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(7736..8302)
                     /gene="efp"
                     /locus_tag="JNC24_003250"
     CDS             complement(7736..8302)
                     /gene="efp"
                     /locus_tag="JNC24_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HAY9159931.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            8344..9372
                     /gene="epmB"
                     /locus_tag="JNC24_003251"
     CDS             8344..9372
                     /gene="epmB"
                     /locus_tag="JNC24_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HAY9159932.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPGELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPILRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
     gene            9762..>9851
                     /locus_tag="JNC24_003252"
     CDS             9762..>9851
                     /locus_tag="JNC24_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9159933.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPR"
BASE COUNT         2517 a         2504 c         2502 g         2328 t
ORIGIN      
        1 aggcgaatgg aataaagaat aacaatgcag aaaatatata aaagggaatc gcactataac
       61 ccattaagta ataagccgat gggctattgg caaatccaaa tacggaagta aagatcatca
      121 atatgagtcc tatcagactc atctttttta tcgtgtgagg catgaaacca tccttcattt
      181 cagcgcgtgt ataacacgtg cccactgaac tgcactgcgc aacattgagc aggcagcgta
      241 aaaaccaata cagaattgat acccggatga tatcaaatat ccagtcttaa attgtggcct
      301 ttaacggaaa aatgaaaaca tatgctacag aatggcgctt atcacgccat tcttcgctgt
      361 taattcagta atttttcaga attatgcccg gtcaacgcta aaggcgatga cttcagccag
      421 tgtctccgcg cccagcgcca acatcaccag acgatcaaca cctaatgcca cgccggaaca
      481 gtcaggcata ccgactttca aggcttcaat cagattctgg tcaatggggt gctgcggcaa
      541 accgcgcgcc gcgcgcttac ggttatcttg ttcaaagcgt tgttgctgct cacgggcatc
      601 cgttaattca tggaaaccat tcgccagctc aataccttta taataaacct caaagcgttc
      661 agcgacccga tgatcttcgg tactgatttg cgccagtgat gcctggctgg ctggaaagtg
      721 gtacacaaag gtcggttttt ctttgccaat atttggctct acgccaaagg taaacagcaa
      781 ttgtagcaac gtgtcgcggt cttcttcggt atcagcaacg ttactcaaat ccagtttcgc
      841 tgcgacttcc cgcagttgcg ttttgtcggc agagagcggg tcaatttcca gataacgcaa
      901 gaaagcttgt tgataagaaa ggctttctgc tgccgggcag tccagcacct gttgtaagag
      961 atcgtccacc tcgttcatca accggtacat atcatagtgc ggtcgatacc actcaagcat
     1021 agtgaactca gggttgtgat aacgccccat ctcttcatta cggaagctgc ggcacagctg
     1081 gaataccggc ccacaaccgg caaccagcag gcgtttcata tggtattccg ggctggtcat
     1141 taaccagaga ttcatcccct gcgaatgccc ggggccaacg aaacgtgtct caaacgggac
     1201 caaatgaata tcggttaccg tcgcctggct catacaaggc gtctccacct ccagcactcc
     1261 acgatcggca aagaaacgac ggatctccgc cataatcgcc gcacgtttta ataagttagg
     1321 aatggatgcg ctcggctgcc aggatgccgt ttcgctcata gttaaatctc cagtttttga
     1381 caagggcacg aagtctactc gcaacgcgac ggcgagacaa attttacgca ggaatcaaac
     1441 agcggtgggc agtgactaaa aaaagcacga tctgatggtt tagtaattaa attaatcatc
     1501 ttcagtgata atttagccct cttgcgcact aaaaaaatcg atctcgtcaa atttcagact
     1561 tatccatcag actatactgt tgtacctata aaggagcagt ggaatagcgt tcgcagaccg
     1621 taactttcag gcacttaccc tgaagtacgg ggctgtggga taaaaacaat ctggaggaat
     1681 gtcgtgcaaa cctttcaagc cgatcttgcc attgtaggcg ccggtggcgc gggattacgt
     1741 gctgcaattg ctgccgcgca ggcaaatcca aatgcaaaaa tcgcactaat ctcaaaagta
     1801 tacccgatgc gtagccatac cgttgctgca gaagggggct ccgccgctgt cgcgcaggat
     1861 catgacagct tcgaatatca ctttcacgat acagtagcgg gtggcgactg gttgtgtgag
     1921 caggatgtcg tggattattt cgtccaccac tgcccaaccg aaatgaccca actggaactg
     1981 tgggggtgcc catggagccg tcgcccggat ggtagcgtca acgtacgtcg cttcggcggc
     2041 atgaaaatcg agcgcacctg gttcgccgcc gataagaccg gcttccatat gctgcacacg
     2101 ctgttccaga cctctctgca attcccgcag atccagcgtt ttgacgaaca tttcgtgctg
     2161 gatattctgg ttgatgatgg tcatgttcgc ggcctggtag caatgaacat gatggaaggc
     2221 acgctggtgc agatccgtgc taacgcggtc gttatggcta ctggcggtgc gggtcgcgtt
     2281 tatcgttaca acaccaacgg cggcatcgtt accggtgacg gtatgggtat ggcgctaagc
     2341 cacggcgttc cgctgcgtga catggaattc gttcagtatc acccaaccgg tctgccaggt
     2401 tccggtatcc tgatgaccga aggctgccgt ggtgaaggcg gtattctggt caacaaaaat
     2461 ggctaccgtt atctgcaaga ttacggcatg ggcccggaaa ctccgctggg cgagccgaaa
     2521 aacaaatata tggaactggg tccacgcgac aaagtttctc aggccttctg gcacgaatgg
     2581 cgtaaaggca acaccatctc cacgccgcgt ggcgatgtgg tttatctcga cttgcgtcac
     2641 ctcggcgaga aaaaactgca tgaacgtctg ccgttcatct gcgaactggc gaaagcgtac
     2701 gttggcgtcg atccggttaa agaaccgatt ccggtacgtc cgaccgcaca ctacaccatg
     2761 ggcggtatcg aaaccgatca gaactgtgaa acccgcatta aaggtctgtt cgccgtgggt
     2821 gaatgttcct ctgttggtct gcacggtgca aaccgtctgg gctccaactc cctggcggaa
     2881 ctggtggtct tcggccgtct ggccggtgaa caagcgacag agcgtgcagc aactgccggt
     2941 aatggcaacg aagcggcaat tgaagcgcag gcagctggcg ttgaacaacg tctgaaagat
     3001 ctggttaacc aggatggcgg cgaaaactgg gcgaagatcc gcgacgaaat gggcctggca
     3061 atggaagaag gttgcggtat ctaccgtacg ccggaactga tgcagaaaac catcgacaag
     3121 ctggcagagc tgcaggaatg cttcaagcgc gtgcgcatca ccgacacctc cagcgtgttc
     3181 aacaccgacc tgctctacac cattgaactg ggccacggtc tgaacgttgc tgaatgtatg
     3241 gcgcactccg caatggcacg taaagagtcc cgcggcgcac accagcgtct ggacgaaggt
     3301 tgcaccgagc gtgacgacgt caacttcctc aaacacaccc tcgccttccg cgatgctgat
     3361 ggcacgactc gcctagagta cagcgacgtg aagattacta cgctgccgcc agctaaacgc
     3421 gtttacggtg gcgaagcgga tgcagccgat aaggcggaag cagccaataa gaaggagaag
     3481 gcgaatggct gagatgaaaa acctgaaaat tgaggtggtg cgctataacc cggaagtcga
     3541 taccgcaccg catagcgcat tctatgaagt gccttatgac gcaactacct cattactgga
     3601 tgcgctggtc tacatcaaag acaacctggc accggacctg agttaccgct ggtcctgccg
     3661 tatggcgatt tgtggttcct gcggcatgat ggttaacaac gtgccaaaac tggcatgtaa
     3721 aaccttcctg cgtgattaca ccgacggtat gaaggttgaa gcgttagcta acttcccgat
     3781 tgaacgcgat ctggtggtcg atatgaccca cttcatcgaa agtctggaag cgatcaaacc
     3841 gtacatcatc ggcaactccc gcaccgcgga tcagggtact aacatccaga ccccggcgca
     3901 gatggcaaag tatcaccagt tctccggttg catcaactgt ggtctgtgct acgccgcgtg
     3961 cccgcagttt ggcctgaacc cagagttcat cggtccggct gccattacgc tggcgcatcg
     4021 ttataacgaa gatagccgcg accacggtaa gaaggagcgt atggcgcagt tgaacagcca
     4081 gaacggcgta tggagctgta ctttcgtggg ctattgctcc gaagtctgcc cgaaacacgt
     4141 cgatccggct gcggccattc agcagggcaa agtagaaagt tcgaaagact ttcttatcgc
     4201 gaccctgaaa ccacgctaag gagtgcaaca tgacgactaa acgtaaaccg tatgtacggc
     4261 caatgacgtc cacctggtgg aaaaaattgc cgttttatcg cttttacatg ctgcgcgaag
     4321 gcacggcggt tccggctgtg tggttcagca ttgaactgat tttcgggctg tttgccctga
     4381 aaaatggccc ggaagcctgg gcgggattcg tcgacttttt acaaaacccg gttatcgtga
     4441 tcattaacct gatcactctg gcggcagctc tgctgcacac caaaacctgg tttgaactgg
     4501 caccgaaagc ggccaatatc attgtaaaag acgaaaaaat gggaccagag ccaattatca
     4561 aaagtctctg ggcggtaact gtggttgcca ccatcgtaat cctgtttgtt gccctgtact
     4621 ggtaaggagc ctgagatgat taatccaaat ccaaagcgtt ctgacgaacc ggtattctgg
     4681 ggcctcttcg gggccggtgg tatgtggagc gcaattattg cgccggtgat gatcctgctg
     4741 gtgggtattc tgctgccact ggggctgttt ccgggtgatg cgctgagcta cgagcgcgtt
     4801 ctggcgttcg cgcagagctt cattggtcgc gtattcctgt tcctgatgat cgttctgccg
     4861 ctgtggtgtg gtttacaccg tatgcaccac gcgatgcacg atctgaaaat tcacgtgcct
     4921 gcgggcaaat gggttttcta cggtctggct gctatcctga cagttgtcac gctgattggt
     4981 atcgttacaa tctaacgtat cgccaatgta aatccggccc gcctatggcg ggccgttttg
     5041 tatggaaacc agaccttatg ttcaaaacga cgctctgcgc cttattaatt accgcctctt
     5101 gctccacatt tgctgcccct caacaaatca acgatattgt gcatcgcaca attaccccgc
     5161 ttatagagca acaaaagatc cccggtatgg cggtggcggt aatttatcag ggtaaacctt
     5221 attactttac ctggggctat gcggacatcg ccaaaaagca gcccgtcaca cagcaaacgt
     5281 tgtttgagtt aggttcggtc agcaaaacat ttacgggcgt gcttggtggc gacgctattg
     5341 ctcgagggga aatcaagtta agcgatcccg caacaaaata ctggcctgaa cttactgcta
     5401 aacagtggaa tgggatcaca ctattacatc ttgcgaccta caccgctggc ggcctgccat
     5461 tgcaggtgcc ggatgaagtg aaatcctcaa gcgacttgct gcgcttctat caaaactggc
     5521 agcctgcatg ggctccagga acacaacgtc tgtatgccaa ctccagtatc ggtttgttcg
     5581 gcgcactggc tgtgaagccg tctggtttga gttttgagca ggcgatgcaa actcgtgtct
     5641 tccagccact caaactcaac catacgtgga ttaatgtacc tcccccagaa gaaaagaatt
     5701 acgcctgggg atatcgcgaa ggtaaggcag tgcatgtttc gccaggggcg ttagatgctg
     5761 aagcttatgg tgtgaagtcg accattgaag atatggcccg ctgggtgcaa agcaatttaa
     5821 ccccccttga tatcaatgag aaaacgcttc aacaagggat acaactggca caatctcgct
     5881 actggcaaac cggcgatatg tatcagggcc tgggctggga aatgctggac tggccggtca
     5941 atcctgacag catcattaac ggcagtgaca ataaaattgc actggcagca cgcccggtaa
     6001 aagcgattaa gcccccaact cctgcagtac gcgcatcatg ggtacataaa acgggggcga
     6061 ccggcggatt tggtagctat gtcgcgttta ttccagaaaa agagctgggt atcgtgatgc
     6121 tggctaacaa aaactatccc aatccagcga gagtcgccgc cgcctgtcag attctcaacg
     6181 ctctacagta aaatttcatc gggtccgaat tttcggacct tttctgcgct tttccttgct
     6241 gtcatctaca cttagaaaaa aaccagtaag gaaacattta tgcgcctgct ccctctcgtt
     6301 gccgcagcga cagctgcatt tctggtcgtt gcctgcagtt ctcctacgcc gccgcgtggc
     6361 gtgacagtag tgaataattt cgacgctaaa cgctatctcg gtacctggta tgagattgcc
     6421 cgttttgatc accgttttga acgtggactg gaaaaagtca ccgcaacata cagtctgcgt
     6481 gatgacggcg gcctgaatgt catcaataaa ggctataacc ctgacagaga aatgtggcag
     6541 cagagtgaag ggaaagcgta ctttaccggc gcaccaacac gcgctgcgct gaaagtgtca
     6601 ttctttggtc ctttctatgg cggttataac gtcattgcac ttgatcggga ataccgccat
     6661 gcgctggttt gcggcccgga ccgcgactac ctgtggatac tctcccgcac gccaaccatt
     6721 tctgacgaag tgaaacagga gatgctggca gtcgcgaccc gggaagggtt tgatgtcagt
     6781 aagtttattt gggtacagca gcccggtagt tagtgagtgc tgagtttcag accaataatc
     6841 cccaatacga ttaacgccag actcgccagg cgcatcgggt tagcggactc accgagcagc
     6901 acaatgccgg tgatggccgc gccgactgcg ccaatacccg tccacacggc ataagccgtc
     6961 cctaccggta acgatttcat cgcccaggca agtagcgcca tactgactat catcgccgtc
     7021 acagtaataa cacttggcgt caaacgacta aagccgtggg tatatttcag gccaacggcc
     7081 cataccactt ccagcagacc agcaataact aagataatcc aggacatatc aggctccgga
     7141 gaattggggc catccccggt gaaaagaggc gtttgcaggt cgtcctgcaa agctgatggt
     7201 tgaacggata ttttgccagg tggcagggga atttcaacta ttacctttgt gactatcgca
     7261 aaaaaacaaa cggcacgaca taactgtcgt accgttattg cttattgctg cgctttcgtt
     7321 gctgcaccag aaatcgcgtt accgccatca gaaatgtctt caccaacgcc acgcgtggtg
     7381 ttgcaggcag ttaatactgt tgaaagcacc agaacagaaa agatcgctgc aattgtcttc
     7441 ttcaccataa cgtcttcctt ttaggtaatg ttatttatgt ttgcctatag caaattaata
     7501 atagacaaca tcgccaaaaa tgatggaaat ctgatgattt ttcggaagag aagaaaaatt
     7561 agctggcagc gcgggagatg gagttgccga gatgtttgat gtcttcgccg aaaccgcgag
     7621 cggtgttaca gcccgtgagc agcgtgctgg caagcaaaac aagaacgata aggcgtttca
     7681 tcatctctga cctgaataag tgatggtgca gcctgcaggc cgcaccacaa ccgcattact
     7741 tcacgcgaga gacgtattca ccagagcggg tatccacttt gatgacttcg ccgatttgta
     7801 cgaacagcgg aactttaacc acagcgccag tagacagggt agccggtttg ccgccagtac
     7861 ctgcggtatc acctttcagg cccggatcgg tatcaacgat ttccagttca acgaagttcg
     7921 gcggagtaac ggagatcggc tgaccattcc acagagttac gatacactct gcctgatcca
     7981 gcagccattt agcgttgtcg ccaattgctt ttgcatcagc agacagctgc tcgaaagttt
     8041 cgttgttcat gaagtgccag aactcaccgt cgttgtacag gtaagtcagg ttcatatcga
     8101 caacatcagc gccttcagcg gaatcagtag atttgaaggt tttttctacg cgagtaccgg
     8161 tcagcagacg acgcagttta acgcgagcaa atgcctggcc tttacccggt tttacgaatt
     8221 cactcgcttc aaccgcgtaa ggttcgccgt ctaacatgat tttaagacca gcacgaaaat
     8281 cgttgctata gtacgttgcc ataaggccct ctgaaatttg ttaattggta gctaagccac
     8341 aaaatggcgc atattgtaac cctaaatacc ccatccagag aagattggtt aacgcaactt
     8401 gccgatgttg tgaccgatcc tggtgaactt ctgcgtcttt tgaatataga cgcggacgaa
     8461 aaactgttgg ccggacgcag cgccaaaaag ctgtttgccc tgcgtgtgcc ccgctcattt
     8521 atcgatcgca tggagaaagg caatccggac gatcctattt tgcgtcaggt acttacctcg
     8581 caagatgagt ttgtcgtcgc gtccggattc tccaccgacc cactggaaga acagcacagc
     8641 gtagtgcctg gtttgttgca taaataccac aaccgggcgc ttttgctggt caaaggcggc
     8701 tgcgcggtaa attgccgcta ttgcttccgt cgccacttcc cctatgccga aaatcagggc
     8761 aacaagcgta actggcagac tgcacttgag tatgttgctg cgcatccgga actggacgag
     8821 atgattttct ccggcggcga tccgctgatg gcgaaagatc acgagctgga ctggttgctc
     8881 acacaactgg aagccatccc gcatatcaaa cgtctgcgga ttcacagccg tctgccgatt
     8941 gtgatcccgg cacgtatcac cgaggcgctg gttgaacgct ttgcccgttc tacgctgcaa
     9001 atcttgctgg tgaatcacat caatcatgcc aatgaggtag atgaaacgtt ccgtcaggcg
     9061 atggctaaat tgcgccgtgt cggtgtcacc ctgcttaacc agagcgttct gttacgtggt
     9121 gtgaacgata acgcacaaac gttggcaaac ctgagtaatg cgttgttcga tgccggcgtg
     9181 atgccgtatt acctgcatgt gctagataaa gtacagggcg cggcgcattt tatggtgagt
     9241 gatgacgaag cacggcagat tatgcgtgag ttgctgacat tggtgtcggg atatctggtg
     9301 ccgaaactgg cgcgagaaat tggcggcgaa cccagcaaaa cgccgctgga tctccagcta
     9361 cgccagcagt aactgattaa acagggaagt taaacattag cctgatgcgc tacgcttatc
     9421 aggcctacat tgtgcattga aattgaatgg atttgcaatg aactgtaggc cggataaggc
     9481 gtttacgccg catccggcaa gagcaacaaa cgcaagtaca gcaacctgtc attaaactgg
     9541 gaaatttaat ctttgcccgt tgcataacct ttaattattc atatacattg ctgttgttac
     9601 tctttatata gccaacacca ataattaacc tttcttttct gtttatatag agtgagtacg
     9661 gctatttatg ttattttaac aactattact accaagccca tttatctggc ttataaatta
     9721 aaattaatta tttaattgag aaatggttag ggagaaccta catggccata agcatcaagg
     9781 gtgttaatac cggtgttatt cgcaagagta ataatttcat tgcactggcg ctgaaaatta
     9841 aagaacctcg c
//
LOCUS       DADMKF010000141         9822 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.144, whole genome shotgun sequence.
ACCESSION   DADMKF010000141 DADMKF010000000
VERSION     DADMKF010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9822
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.144"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(39..1034)
                     /gene="gapA"
                     /locus_tag="JNC24_003253"
     CDS             complement(39..1034)
                     /gene="gapA"
                     /locus_tag="JNC24_003253"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAY9159934.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            1376..1789
                     /gene="msrB"
                     /locus_tag="JNC24_003254"
     CDS             1376..1789
                     /gene="msrB"
                     /locus_tag="JNC24_003254"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HAY9159935.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSQTKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            1831..2103
                     /locus_tag="JNC24_003255"
     CDS             1831..2103
                     /locus_tag="JNC24_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HAY9159936.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKANCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            complement(2264..2905)
                     /gene="pncA"
                     /locus_tag="JNC24_003256"
     CDS             complement(2264..2905)
                     /gene="pncA"
                     /locus_tag="JNC24_003256"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HAY9159937.1"
                     /translation="MPPRALLLVDLQNDFCAGGALAVPEGDSTVDVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLDGLPQTFWPDHCVQNSEGAQLHPLLNQ
                     KAIAAVFHKGENPLVDSYSAFFDNGRRQKTSLDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(2916..3932)
                     /gene="ansA"
                     /locus_tag="JNC24_003257"
     CDS             complement(2916..3932)
                     /gene="ansA"
                     /locus_tag="JNC24_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HAY9159938.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMGGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            complement(4099..5955)
                     /gene="sppA"
                     /locus_tag="JNC24_003258"
     CDS             complement(4099..5955)
                     /gene="sppA"
                     /locus_tag="JNC24_003258"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HAY9159939.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKPVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            6116..6667
                     /locus_tag="JNC24_003259"
     CDS             6116..6667
                     /locus_tag="JNC24_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HAY9159940.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGRERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCEENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            6784..7827
                     /gene="selD"
                     /locus_tag="JNC24_003260"
     CDS             6784..7827
                     /gene="selD"
                     /locus_tag="JNC24_003260"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HAY9159941.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDATEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYDAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKATAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            7832..9793
                     /locus_tag="JNC24_003261"
     CDS             7832..9793
                     /locus_tag="JNC24_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAY9159942.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIIPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERALDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLLQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDVVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKILRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEVGSGAIA"
BASE COUNT         2434 a         2453 c         2617 g         2318 t
ORIGIN      
        1 ccgaagtcgc tctttttaga tcacagtttc atctcaactt atttggagat gtgagcgatc
       61 aggtccagaa ctttgttgga gtaaccggtt tcgttgtcgt accaggatac cagtttcacg
      121 aagttgtcgt tcagagcgat accagcttta gcatcgaaca cggaagtgca aacttcgccg
      181 ttgaaatcgg tagatactac gtcatcttcg gtgtagccca gaacgccttt catttcgcct
      241 tcagcagcgg ctttaacggc agctttgatc tgctcgtaag ttgcagcttt ttccagacga
      301 acggtcaggt caactacgga tacgttcggg gtcggaacgc ggaacgccat accagtcagt
      361 ttgccattca gttctggcag tactttacct acagctttag cagcaccggt agaggacggg
      421 atgatgttct gggaagcgcc gcggccgccg cgccagtctt tgtgagacgg gccatcaacg
      481 gttttctgag tagcggtagt agcgtgaacg gtggtcatca gaccttcgat gatgccgaag
      541 ttatcgttga taactttagc cagcggagcc aggcagttgg tggtgcagga agcgttggaa
      601 acgatgtcct ggccagcata tttgtcgaag ttagcgcctt taacgaacat cggagtgttg
      661 tctttagacg gaccagtcat aaccactttc ttcgcaccag cggtgatgtg tttacgagca
      721 gtttcgtcag tcaggaacag accagttgct tcagcgacaa cgtcaacacc aacttcgtcc
      781 catttcaggt tagccggatc acgttcagcg gtaacacgga tttttttacc gttaacgatc
      841 agatgaccgt ctttcacttc aacggtaccg tcgaaacggc cgtgagtgga gtcatatttc
      901 agcatgtatg ccatgtaatc agcgtctaac aggtcgttga ttgcaacgat ctcgatgtca
      961 gaacgtttct gagcagcacg gaaaacaatg cgaccgatac ggccaaaacc gttgatacct
     1021 actttgatag tcatatattc caccagctat ttgttagtga ataaaaggtt gcctgtaaaa
     1081 ttacaaaaac cttacgcagc gtcaagcgga atcgtgtcaa tcattgcgac aaatcaatcc
     1141 tgtgcctaag cattacgcga ctgactcgcc tcactcttcc tttgggcttg aaaccacatg
     1201 gggtcggcgc cccgaatttt aaagggcaat tacgataaaa atgtgatttt catcacgatt
     1261 taggtgcagc catttcgtcg agatcaagtt taggacaaaa ttttgccagt tggtgttaat
     1321 gttttgttag aatcagtcac gtaatgtgag cacgattaaa gtgagatgtg agcaaatggc
     1381 taataaacct tcggcagaag aactgaaaaa aaatttgtcc gagatgcagt tttacgtgac
     1441 gcagaatcat gggacagagc cgccatttac gggtcgttta ctgcataaca agcgtgacgg
     1501 cgtatatcac tgtttgatct gcgatgcccc gctgtttcat tcccaaacca agtatgattc
     1561 cggctgtggt tggcccagtt tctacgaacc ggtgagtgaa gaatccattc gttatatcaa
     1621 agacttgtca catggaatgc agcgcataga aattcgttgc ggtaactgtg atgcccatct
     1681 ggggcatgtc ttccctgacg ggccgcagcc aacgggcgaa cgttattgtg ttaactctgc
     1741 ttctttacgc ttcactgatg gcgaaaacgg cgaagaaatc aacggttgaa aaaacgattc
     1801 agcaacttat tccacaggag cggaaaaatt atgaatcttg atgacattat caacagcatg
     1861 acgcctgagg tataccagcg tttgtcgacc gccgttgaac tggggaaatg gcccgatggc
     1921 gttgcgttga ccgaggaaca aaaagcgaac tgcctgcaac tggtgatgct gtggcaagct
     1981 cgccataata ccgaagcaca gcatatgaca attgacacaa atggtcagat ggtcatgaag
     2041 agcaaacagc agttaaaaga agattttggt atctcagcaa agcctattgc tatgtttaag
     2101 tgaatgagtt atctagtatt taagcctgtt attaagggag ggcaaatata cttgttagga
     2161 cgtcgtgttt tagctgcaag taggttaaat gcaacactga caagtattat ctggcctaca
     2221 atgtcattga ggaaatgtag gccagagatg aattcaacta atattacccc tgtgtctctt
     2281 cccagtctgc cagcgtatat agcgttgccc cagctgctga catctccata aacgcgtgcg
     2341 cactgtcctg gggctggata ttcacgccac gacaaccatc ggtaatcaca tttaccttat
     2401 aacctaactg taacgcgtcc agcacggtaa acttcacgca atagtcagta gccaggccca
     2461 taacgatcaa ttcatcgatt tcatgatcgc gtaaccagtc atcgagcgag gttttctgcc
     2521 gacggccgtt atcaaaaaag gcactgtaac tgtcaactaa aggattttcg cctttatgga
     2581 acaccgctgc gatcgctttt tggttcagta acggatgtaa ttgtgcgcct tcactgttct
     2641 gcacacagtg atctggccag aaggtttgtg gcaaaccgtc gagttggcct ggcgtataag
     2701 gctctacacc gtgctgactg gcaaaactgc cgtgattcgc cgggtgccag tcctgactgg
     2761 cgataaccgc ttcaccgcgc gactggcacc agtcaatcag gcggttagcg acatccaccg
     2821 tactgtcacc ttccggcacg gcgagcgcgc caccagcaca gaaatcattt tgtaaatcga
     2881 ccagtaacag ggcgcgaggg ggcattacag tctccttaat catccggcgt cagttcgccg
     2941 cgcaggtttt ggctcatggc cttgcgaatg gtttcagtat ccagttcctg gctcagcagg
     3001 taatgcagtt tggttagcgt ggcttctaca gtcatatctg caccgccaat aacgccggca
     3061 tgggcgaggg cgttaccggt ggcataacca cccatattca ctttaccgga catacattgt
     3121 gtcaggttga ccaccacaat accgcgatcg ctggcttctt gtaattcctg caggaaggct
     3181 ttgttttgtg gcgcattacc cacgccatag gagcgcagaa tcaatgcttt caccggttgg
     3241 cgcagaaaat tgcgcacgac gtcagcagaa atccctggat aaatcgtcac tacgccaatt
     3301 ggttgtggcg tgattggatg aacgattaat tccccttcac cgtgcggggc gggtggcgta
     3361 ttcaaacgac gaatatggat accagcttcc agtaacggag gaaggtttgg agaggcaaac
     3421 gcatcaaaac catcggcatg ggctttggta gtgcggttgc cgcgatacaa tcggttattg
     3481 aaaaagagcg ttacttcgtt gatcggataa ttcgccgcaa cgtacaacgc attcagcaga
     3541 ttaatttgtc cgtcagagcg taactcagcc agcgggattt gtgaccctgt cacaatgacc
     3601 ggtttaccga gattctcgag catgaacgac agcgcagagg cagtatacgc catcgtgtcg
     3661 gtgccgtgca ggatgacaaa accatcatag tcgtcatagt gcgctttaat atcttcagca
     3721 atatgctgcc agtcttctgg cgtcatatca gaagaatcca tcagcggcgt atattcatga
     3781 atggtgaaat ctggcatctc cgggcgatgg aattccggca tcagcgccag ttggcgttgt
     3841 agatgacctg acaccggaat ataaccctgc tcggaacgct gcatcccgat ggtcccgccc
     3901 gtgtaggcga cgtaaattga tttcttttgc atgatattga tatgaatgat atcgaaagag
     3961 caaaagtata tacgtgaggc aggaggagcg cacctgatac tatgcggata aggaaatatg
     4021 gggcgagaca gcgggaagag gattcgtcga cgtaaaaaat aatgtggccg acgatctgtc
     4081 ggccactcag tacaagactt aacgcacgtt ggcgcaggtc aggcaaaacg cataacggtt
     4141 ttgtgggtcg ttaaacgcgg ccagcttgtc gctttcactt ttaacggtag aggctaccga
     4201 ggcaagcggt gcaggtaaca tggcctggaa cgcatctggc aacattgccc ggacagaacc
     4261 agacatgttg tccatcactt tgtcgaagaa ggtcggttca tcaacgtagt attccagatg
     4321 ccactgtttc acttttgcca gctctgctgc tttggcgacc gcatcatcga aatccccgag
     4381 actatcgacc agcccgttag cttttgcatc ctgaccggtc cagacgtggc cctgggcgat
     4441 tttatcaatc tgctccggcg tcgaatgacg cgcatcagca accagcgtga taaagcgttt
     4501 atagccattc tcaatgctta attgcatcat ctgctgcgct tccggcggca gtgccctggt
     4561 gatagaaaca tccgccagcg gtgaagttga gacaccatca gtatgaacac caatcgaatc
     4621 cagactattt tctacggtgg tgatcacgcc gaagataccg atagaaccgg tcagggtgct
     4681 ggggttagcc acaatgtaat tagctggcgt ggaaatccag taaccaccag atgccgccat
     4741 gccgcccatc gatacaacca caggcttacc cgctgcccgg gctgctgcca gttcagcgcg
     4801 aatcacttca gacgcggtaa cgctgccgcc tgggctatta acacgcagga caatcgcttt
     4861 cactttcggg tcaaggcgag cgtcgcggat ttgtgccgca gtggtatcac cgccaacatt
     4921 cccctgagtt tcctcgccat ccataattgc gccattagcg aagacgacac cgatgctgtc
     4981 accggtatct gccggcgttt tcaatgcgta atcgtaataa ctgatggcgc gataattttt
     5041 atcagtctta ctccagccga actctttggt tagagctttt tcgatttcgg cactcgatgc
     5101 cagtgcatcg accagcttgt tttccagtgc atatttcgcg gtatcgccac cggttttggt
     5161 taaaccctca agcaaccctt gcgcgccagg gaatacctgc tgagcaggga tctgccggtt
     5221 agcggcaaca gtattcagat agttttgcca cagctcacca atccagcggc tgtcagcttc
     5281 gcgggctgcc ggtgacatgt catcacgaat aaacggttca acggcagatt tatacgtacc
     5341 cacgcggaac acatgggtgg aaactttcag cttatccagc aacgatttgt agtacagacc
     5401 gttggtagca aaaccgtgca aatccaccac gccttgcggt gacagccaaa ttttattggc
     5461 gaaactggcg agataatatt gcccctggct gtagttctcg ccaacggcat aaaccggttt
     5521 cccgctgtca cgaaactctt tcagggcttt gccgatgtac tgcatagacg gctggtcgcc
     5581 gcctgcgaag tttttcagat ccatcacgat accggtgata ttgcggtcgt ccttcgcctg
     5641 gcgaatggtg ttgacgatat caaacagtga gttttcctgc agacgatcgg aactggcacc
     5701 aagcagctgg cggcttaatt tactaaaccg ctgagaactg tcgggtttat cgacgatcac
     5761 accagaaatg tccagcagca gtgcgccacg actggccgtt tctttcgaat caccaccact
     5821 gacctgcatc cagatcccca caccaaccag tacgaggaaa ataaagaaca ggttaagtac
     5881 catttcacgg acgaaattca gcagacgcca cgtccattta aaaaatccgg caataaatcg
     5941 ccaaagggtt cgcatgtatt ctcccaactt aaggtcacac ctgtcacagg cgcaatagat
     6001 gtggttatcg taatgacaca accggcactt gtcagcagga attgcggact gtgctgtaac
     6061 aaaatccact ctcgtgttaa ttttgtgaat aaatatcacg acaggagtta atcaaatgga
     6121 tgcactcgaa ctattgatca atcgccgtag cgcctcccgc ctggctgaac ccgcgccaac
     6181 gggtgaacaa ctgcaaaaca tcctgcgtgc gggtatgcgt gcgccggacc ataagtccat
     6241 gcaaccgtgg catttttttg tgattgaagg ggaagggcgc gagcgtttca gcgccgtact
     6301 ggaacagggg gcgattgctg ccggtagtga tgacaaagct atcgacaaag cccgtaatgc
     6361 gccgttccgc gcaccgctca tcatcacggt ggtggcgaaa tgcgaagaaa atcataaagt
     6421 cccgcgatgg gaacaggaaa tgtctgccgg atgcgcggtc atggcgatgc aaatggcagc
     6481 cgttgcccag ggatttggcg gcatctggcg cagtggcgca ttaactgaaa gtccggtagt
     6541 gcgtgaagca ttcggttgcc gtgagcagga taaaattgtc ggttttctct acctcggcac
     6601 gccgcagttg aaagcatcta cgtcgattaa cgtcccggac ccgacgccgt ttgtaactta
     6661 tttctgataa ttgtcgcgaa actgtctgga ttgtgagcaa aggcgcagga attcagacac
     6721 tctcacttat cacttcacgg aatgagggtt accatacccg gattgcattg acaggagatg
     6781 tccatgagcg agaactcgat tcgtttgacc caatacagcc acggagctgg ttgcggctgt
     6841 aaaatttccc caaaagtgtt ggaaaccatc ctgcacagtg agcaggcgaa gtttgttgat
     6901 ccgaatttgc ttgtgggtaa tgagacccgc gacgatgcgg cggtgtacga tctgggcaat
     6961 ggcaccagcg ttatcagtac caccgacttc tttatgccga tcgttgataa tcctttcgat
     7021 tttggccgca ttgcggcgac taacgccatc agcgatatct tcgcgatggg gggcaaaccg
     7081 attatggcga ttgcgatcct cggctggccg attaacaaac tttccccgga aattgcccgc
     7141 gaagtaaccg aaggtggacg ctatgcatgc cgtcaggcgg gtattgcgct ggctggcggt
     7201 cactccatcg atgcgacgga gccgattttt ggtctggcgg taacggggat cgtaccgacc
     7261 gagcgggtga agaaaaacag taccgcacaa gccggatgca aactgttcct gacgaaaccg
     7321 ctggggatcg gcgttcttac cacggctgag aaaaaatcac tgctgaaacc agaacatcag
     7381 ggactagcga cggaagtgat gtgccggatg aacatcgcag gcgcgtcctt tgccaacatc
     7441 gaaggcgtaa aagcgatgac cgacgttacg ggttttggtc tgctgggcca tttgagcgaa
     7501 atgtgtcagg gcgcaggtgt gcaggcgcgc gttgactatg acgcgatccc aaaattgccg
     7561 ggggttgaag agtacattaa gttgggcgca gtgcctggcg gcaccgaacg taactttgcc
     7621 agctacggtc atctgatggg tgaaatgccg cgtgaagtgc gcgatttgct gtgcgatccg
     7681 caaacttctg gcggtttgct gctggcggtc atgccggaag cagaaaatga ggtcaaagct
     7741 acagccgccg agtttggcat tgaactgacg gcaattggcg aactggtgcc agcgcgcggc
     7801 ggtcgtgcca tggttgagat tcgttaattc aatgcggttg tttattgccg aaaaaccgag
     7861 tctggcgcgc gccattgctg atgtcctgcc caaaccgcac cggaaaggcg atggctttat
     7921 cgagtgcggt aatggtcagg tggtgacctg gtgtatcggt cacctgcttg agcaggcgca
     7981 gccagacgcc tacgacagcc gctatgcgcg ctggaatctt gcggatttgc cgattatccc
     8041 ggaaaagtgg caattacagc cccgaccctc cgtgaccaaa caacttaacg tcatcaaacg
     8101 cttcctgcat gaagccagcg aaatcgttca cgccggggac ccggatcgtg aagggcaact
     8161 gctggtggat gaagtgctgg actatctgca actggcaccg gaaaagcgcc agcaggtgca
     8221 gcgttgcttg ataaacgacc tgaacccgca ggcggttgag cgggcgctcg accgtcttcg
     8281 gtccaacagt gagtttgtgc cattgtgcgt ttctgcgctg gcgcgagcgc gtgccgactg
     8341 gctgtacggc atcaatatga cccgtgcgta taccattctc ggtcgcaatg ccggttatca
     8401 gggcgtactt tccgtgggac gcgtgcagac acccgtgctc gggctggtgg tgcgccgcga
     8461 tgaagagatt gaaaacttcg tggcgaaaga cttctttgaa gtcaaagcac atatcgtgac
     8521 acctgccgat gagcggttta ccgctatctg gcaaccgagc gaagcgtgtg aaccgtacca
     8581 ggatgaagaa gggcgcttgt tacatcgtcc actggcggag catgtggtta accgcattag
     8641 tggtcaacca gcaattgtca ccagctataa cgataaacgg gaatcagaat ccgcgccgct
     8701 gcctttttcg ctttcggcgt tgcagattga agcggcaaaa cgctttggtc tgagcgcgca
     8761 gaacgtgctt gatatctgcc agaagctgta cgaaacgcac aagctaatca cttatccgcg
     8821 ttccgattgt cgctatttgc cagaagaaca ttttgccgga cgccacgcgg tgatgaatgc
     8881 catcagcgtt catgcaccag atctgttgct gcagccagtg gtagatccag atatacgcaa
     8941 ccgctgttgg gatgacaaaa aggtcgatgc acaccacgcc atcatcccga ccgcacggag
     9001 ttcagccatc aacctgacgg agaacgaagc gaaggtctat aacctgattg cccgtcagta
     9061 cttgatgcag ttctgcccgg atgtggtgtt ccgcaagtgt gttatcgaac tggacattgc
     9121 caaaggcaaa tttgtcgcta aagcgcgttt tcttgctgaa gcaggctggc gcacgctgtt
     9181 aggcagcaaa gagcgcgatg aagaaaacga cggtacgcca ttgcctgtgg tggcgaaagg
     9241 cgatgagttg ctgtgtgaaa aaggtgaagt ggtagagcgg caaacccagc cgccgcgcca
     9301 ttttaccgat gcaacactgc tttcggcgat gaccgggatc gcgcgctttg tgcaggacaa
     9361 agatctgaaa aagatccttc gtgcgaccga tggtctgggg acagaagcaa cccgtgccgg
     9421 gattattgaa ctgctgttca agcgtggttt tctgaccaaa aaagggcgct atatccactc
     9481 taccgacgcc ggaaaagcgt tattccattc gctgccagaa atggcgacgc gaccggacat
     9541 gaccgcgcac tgggaatcgg tgctgacgca aatcagcgaa aagcagtgtc gctatcagga
     9601 ctttatgcag ccgctggtgg ggacgctata tcagcttatt gatcaagcca aacgtacgcc
     9661 ggtgcggcag tttcgcggca ttgtggctcc gggcagtggt ggcagtgctg ataagaaaaa
     9721 ggctgcaccg cgtaaacgta gtgcgaaaaa aagtccgcca gcagatgaag tcggaagcgg
     9781 ggcgatagcg taagcgagtg aatctttcgt gctattcgag tc
//
LOCUS       DADMKF010000142         9811 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.145, whole genome shotgun sequence.
ACCESSION   DADMKF010000142 DADMKF010000000
VERSION     DADMKF010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9811
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.145"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            18..347
                     /locus_tag="JNC24_003262"
     CDS             18..347
                     /locus_tag="JNC24_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000555422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9159943.1"
                     /translation="MVHSENQSKGVHYAKSLRLLEINHAHLQLMESLLDEGKKHNIFK
                     PDIDPLQVNINIAALGGYYLINQHTLGLVYHISMVSPQALEARRKVIKETILSWLLVD
                     PSSTAHE"
     gene            complement(331..969)
                     /gene="ytfB"
                     /locus_tag="JNC24_003263"
     CDS             complement(331..969)
                     /gene="ytfB"
                     /locus_tag="JNC24_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YtfB"
                     /protein_id="HAY9159944.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNAPVVTREAQLDIQSQSQPPTEEQLRAQLVTPQNDPDQVAPVAPEPIQ
                     EGQPEEQPQTTQTQPFQPDSGIDNQWRSYRVEPGKTMAQLFRDHGLPATDVYAMAQVE
                     GAGKPLSNLQNGQMVKIRQNASGVVTGLTIDTGNNQQVLFTRQPDGSFIRAR"
     gene            1188..1808
                     /locus_tag="JNC24_003264"
     CDS             1188..1808
                     /locus_tag="JNC24_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HAY9159945.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            2117..3529
                     /gene="cycA"
                     /locus_tag="JNC24_003265"
                     /gene_synonym="dagA"
     CDS             2117..3529
                     /gene="cycA"
                     /locus_tag="JNC24_003265"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HAY9159946.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(3574..4236)
                     /gene="ytfE"
                     /locus_tag="JNC24_003266"
     CDS             complement(3574..4236)
                     /gene="ytfE"
                     /locus_tag="JNC24_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HAY9159947.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(4344..5309)
                     /locus_tag="JNC24_003267"
     CDS             complement(4344..5309)
                     /locus_tag="JNC24_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HAY9159948.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALVCIGIGLACVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNIQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            complement(5417..6277)
                     /gene="qorB"
                     /locus_tag="JNC24_003268"
     CDS             complement(5417..6277)
                     /gene="qorB"
                     /locus_tag="JNC24_003268"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HAY9159949.1"
                     /translation="MIAITGATGQLGHYIIESLMKTVPASQIVAIVRNPAKAQALAAQ
                     GITVRQADYGDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYAAAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDNSKTLSKLIGRPTTTLAE
                     SVSHLFNVNN"
     gene            6366..6746
                     /locus_tag="JNC24_003269"
     CDS             6366..6746
                     /locus_tag="JNC24_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAY9159950.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            complement(6864..8807)
                     /gene="cpdB"
                     /locus_tag="JNC24_003270"
     CDS             complement(6864..8807)
                     /gene="cpdB"
                     /locus_tag="JNC24_003270"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HAY9159951.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKTGDVHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VVVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIT
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIDPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINTNAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAADKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            8997..9737
                     /gene="cysQ"
                     /locus_tag="JNC24_003271"
     CDS             8997..9737
                     /gene="cysQ"
                     /locus_tag="JNC24_003271"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HAY9159952.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLTPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKQIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
BASE COUNT         2267 a         2485 c         2551 g         2508 t
ORIGIN      
        1 acccttggtt tttaaaaatt gtgcatagtg aaaatcaaag taaaggtgtg cattatgcaa
       61 aatcactgcg tctcttagaa attaaccatg cgcacttgca gttaatggaa tccttattgg
      121 atgaagggaa gaaacacaat atttttaagc cagatatcga cccattgcag gtgaatatta
      181 atattgctgc gcttggggga tattatttga tcaaccagca tacgcttggc ctggtttatc
      241 acatcagtat ggtttcaccc caggcactgg aagccagacg taaggtcatc aaagagacaa
      301 tccttagctg gcttttggtt gacccttcat ctaccgcgca cgaataaaac tgccatccgg
      361 ctggcgggta aacaggacct gttgattatt ccccgtatca atggttaagc ccgtcaccac
      421 gccgctggcg ttctggcgga ttttcaccat ctgcccattt tgcaaattac tcagcggctt
      481 acccgcgcct tctacctgcg ccattgcata gacatcggta gcgggcagtc cgtggtcgcg
      541 gaacagctgc gccatcgttt taccgggttc gacacggtaa gagcgccatt gattatcaat
      601 tccgctatct ggctggaatg gttgcgtttg ggtagtttgc ggctgttctt ctggctggcc
      661 ttcctgtata ggttctggag cgaccggagc cacctgatca ggatcatttt gtggtgtgac
      721 taattgcgcc cgcagctgtt cttctgtcgg cggttgtgat tgcgactgaa tatctaactg
      781 cgcttcacgc gtcaccaccg gcgcgttggg cgtatcatca gatggaagca gaaatccgac
      841 caccaataca atggcggcaa taatgatccc ccgacgatgc atcggcggca gcgggtccat
      901 gaagcgaaaa ttgtccggcg cgtgccagac tttctccagg gttggtttta gttcaaagcg
      961 cccgggcatg gtttcctcct gctccgcgtc ttgttcctca atcatagcct atgaataagc
     1021 taacgctatg atgtccgtgg taaacccgcc tttattatat tcatacggga tattgctatt
     1081 gtttcttttt ccccgggatt tgtcatcatt cctgcgacaa agttttaccc aaagaagtgt
     1141 ggctgatatg ctgcccgcta cttttatacc ctaagaaagg aaatacgatg accaccccaa
     1201 cttttgacac catcgaagcg caagcaagct acggcattgg tttgcaggta gggcaacaac
     1261 tgagtgaatc tggcctggaa gggctgctgc cagaagcact ggttgcaggt attgccgatg
     1321 cgctggaagg caaacatccg gctgtgccgg ttgatgtggt gcatcgcgcg ctgcgtgaaa
     1381 tccacgaacg cgccgatgcc gttcgtcgtc agcgtttcca ggcgatggct gctgaaggtg
     1441 tgaaatacct ggaagaaaac gccaaaaaag aaggtgtgaa tagcaccgaa tctggcctgc
     1501 aattccgcgt gatcaaccag ggtgaaggcg caattccggc acgtaccgac cgcgttcgtg
     1561 ttcattacac cggtaaactg atcgacggca ccgtgtttga cagctccgtt gctcgtggtg
     1621 aacccgctga attcccggtt aatggcgtga tccctggctg gattgaagca ctgactctga
     1681 tgccggtagg ttctaaatgg gaactgacta tcccgcagga actggcatat ggcgagcgcg
     1741 gcgcaggcgc atccatccct ccgttcagca ccctggtgtt tgaagtcgaa ctgctggaaa
     1801 tcctctaagc agcgcattct gttcccctcg aaccagaggg gagcaggcat tcagcaataa
     1861 acccttcagt ttgccaaacg gcgctatttt gtgttgcaaa gaccccgtaa gcgtgtattt
     1921 ttgtgagctg tttcgcgtta tcaccgtgat atgacactca ctttaaacat aaaattaaca
     1981 ttagatctaa atcttagtat tcatcccgcg tattgttacc taatatcgat gagtcccgat
     2041 acagattcgt cgtatcatag actgactaaa ggccgtagag cctgaacaac acagacaggt
     2101 acaggaagaa aaaaacatgg tagatcaggt aaaagtcgtt gccgatgatc aggctccggc
     2161 tgaacagtcg ctacggcgca atctcacaaa ccgacatatt cagcttattg ccattggcgg
     2221 tgccattggt acggggttgt ttatggggtc cggcaaaacg attagccttg ccgggccgtc
     2281 gatcattttc gtttatatga tcatcggttt tatgctcttt ttcgtgatgc gggcaatggg
     2341 ggaattgctg ctttcgaatc tggaatacaa atcttttagt gacttcgctt ccgatttact
     2401 cgggccgtgg gcaggatatt tcaccggctg gacttactgg ttctgctggg ttgtaaccgg
     2461 tatggcagac gtggtggcga tcactgctta tgctcagttc tggttccccg atctctccga
     2521 ctgggtcgcc tcgctggcgg tgatagtgct gctgctgacg ctcaatctcg ccaccgtgaa
     2581 aatgttcggt gagatggagt tctggtttgc gatgatcaaa atcgtcgcca ttgtgtcgct
     2641 gattgtcgtc ggcctggtta tggtggcgat gcactttcag tcaccgactg gtgtggaagc
     2701 gtcattcgcg catttgtgga atgatggcgg ctggttcccg aaaggtttaa gtggcttctt
     2761 tgccggattc cagatagcgg ttttcgcttt cgtagggatt gagctggtag gtacaaccgc
     2821 tgcggaaacc aaagatccgg agaaatcact gccacgcgcg attaactcca ttccgatccg
     2881 tatcattatg ttctacgtct tcgcgctgat tgtgattatg tccgtgacgc cgtggagttc
     2941 ggtagtcccg gagaagagcc cgtttgttga actgtttgtg ttggtaggtt tgcctgcggc
     3001 tgccagcgtg atcaactttg tggtgctgac ctctgcggcg tcttccgcta acagcggcgt
     3061 cttctctacc agccgtatgc tgtttggtct ggcgcaggaa ggtgtggcac cgaaagcgtt
     3121 cgctaaactt tctaagcgcg cagtacccgc gaaagggttg accttctcgt gtatctgtct
     3181 gctcggcggc gtggtgatgt tgtatgtgaa tcctagcgtg attggcgcgt tcacgatgat
     3241 tacaaccgtt tccgcgattc tgtttatgtt cgtctggaca attatccttt gctcgtacct
     3301 ggtttaccgc aaacagcgtc ctcatctgca tgagaagtcg atctacaaga tgccgctcgg
     3361 caagctgatg tgctgggtat gtatggcgtt ctttgtgttc gtggtcgtgt tgctgacact
     3421 ggaagatgac actcgccagg cgctgttggt caccccgctg tggtttatcg cactggggct
     3481 gggctggttg tttattggta agaagcgggc tgctgaactg cggaaataac cgcattatca
     3541 tgctggatgg cgcaatgcca tccagctttt agattactca cccgccagcg cgcgtgggaa
     3601 cagtacattg ttttccaggc tgatgtgatc catcaggtca tcaatcagtt cattaatacc
     3661 gttatacatc gctttccagg tggtgcaggc ttccggcggc ggtgtgacgt tattggtggt
     3721 gtgtttaatc acttccagca gttcgcccgc ttcatcgtgc tcgctttcca tcacgctgat
     3781 tggtcccatt gcctggctgc ccatgccttg tttgatcatc gggaagagga tctgctcttc
     3841 tttcatcatg tggctggata gctcttcatg cagcatagtc aggtattttg tcagcccttt
     3901 tggcacgctc ggtttgtcgg cgtgaacgcg ctcgacttta gtcgcttgca ggatcagctc
     3961 cggcagttgc tcgcggtgac gatcgtggta gcgcacgatg atatggtcaa tgatttctgc
     4021 cagcggggcg ctacgccagt ctttctcaat cggttgttca gcaagctttg ccagttcagc
     4081 ttcaatgacc tcaacatcca gttctttacg tgccgccgcg cgcgccagcg tttgcttacc
     4141 gccacagcag taatccatat catatttacg aaacagagct gaagcgcgag gaatagagag
     4201 cgccagttca cctaaaggtt ggtcgcgata agccatagct gatacctcat tcttaataat
     4261 ataagatgta ttttaaatgc atctttaagg caaaagctat aacccttacg tagcagaggc
     4321 ttatttaact cactgccagc gtgtcaactt tctgagacag attcagttaa aggtttttct
     4381 ggttttgctc tgaccgcaat gaccacgcca atcactaaca gcgcgatacc gctcagcgtc
     4441 attagcggcg gcatttgctg gcggagtaaa aaggtgtaca gcaaacctgc cagcgtttcg
     4501 aaaacaatca gcggcccgag aatcactgtc ggtaatcgct ggctggcgac gttccagcag
     4561 agtgcgccaa cccatgagca aagcacggct atcgcaacca tcagactaat aaacaccagc
     4621 ggacgggggc caaaaggtaa ggagaagtcc ggcgtttgta tattcagcca gtaacaggcg
     4681 acgagatagc cgatgagaga aaccggcagt gtgaccagcg cctgcgccgt tgcccacatc
     4741 atcggatgtt tgtcgggatt ttcccgcagc cagcgggcgt tgcgcagggc ataccacgcc
     4801 cagcagacca cggaaactaa cgccagcacg atgccagagg tataacgtgc ccagtcaaaa
     4861 tcggggagtc cgtggtttaa ctcagcaata ttcacacacg ccaggccgat gccaatacaa
     4921 accagtgccg gggcgagttt tccccatgcg agtttgccgt cgcgctggct ataaagcaga
     4981 ttggcaaaaa cgggaatcac caccggcagg gtgccgataa tcatcgtgga aacaggcgcg
     5041 ccagtacgtt gaatggcact ggcaaggcag aagtaataga taaggttgcc catcatggtg
     5101 agcatcaagg cggtaagcca gtcgcgacgc gccaactgac gcagacgcac gcgtcccagc
     5161 caggcaatgg gcagcgcaat taaccctaac gccagataac gccccatcga ctgcaacatc
     5221 gccgggtatt ccggtacgat caacggcccg acaaaaataa gcccccacat caaccctgct
     5281 aacagggcgt acagcacgcc gctaatcatt actggcatcc attggtctgt cagaagaaag
     5341 ttcaggaagc agacatgatt gctcccggca tcccgtcatt atcagagagg atgcgggagg
     5401 atgccacttt aattaactag ttattaacat taaaaagatg gcttacgctt tcggctaacg
     5461 ttgtcgttgg gcggccaatc aatttgctaa gcgttttgct gttatcaaac agaccacctt
     5521 tcgatgcgcc aacgtcagaa tccgccagca tatccgccag tccgtcgggc agtccgacgc
     5581 ttttcagtgc cgcggcgaaa tcggcttcgc tcagattttg ataggtaacc tgtttgccgc
     5641 tctgtttggt cagctctgcc gctaactgtg tcaacgtcca ggcactatcg cccgccagtt
     5701 cgtaaacctt gccttcgtga ccggcttcgc taatcacgcg tgccgcagct gccgcataat
     5761 ctgcccgcgt tgctgaggcg attttgccat cgcccgccgc accgataaat acgccgtgtt
     5821 ccagtgctgc cggggcgctg gcgaggtagt tttcgctgta ccagccgttg cgcagcaggg
     5881 tgtaaacgat gccagaatca gccagcattt tctccgtctc aatgtgctca tcggcgaggc
     5941 cgagcgggga ggtatctgca tgtagcaggc tggtataagc gataaatttc acgccagccg
     6001 cctttgcggc attaataaca ttacgatgct gcggggcacg ttgacccact tcgctggaag
     6061 agatcagcag tagtttttcc actccctgaa gtgcagatgt cagtgcggct tcatcgccgt
     6121 agtcagcctg acgcacggta atgccttgtg ctgctagagc ctgggctttt gccggattac
     6181 gaacgatagc cactatttgg ctggcaggaa ccgttttcat caaggattca ataatatagt
     6241 gaccaagttg gccagtggca ccagtaatag cgatcatggg aagtctccat cgttttgtct
     6301 tgtgtttaaa tacgctaaca cctaaactta cttttagtaa gtacgtacaa aaaggtaagt
     6361 atgaaatgag tcaggttagc ctgtcgcaac aactgaaaga gggtaatctc tttgcggaac
     6421 agtgcccgtc gcgcgaggtg ttgaaacacg tcaccagccg ttggggggtg ttgattctgg
     6481 tggcgctacg cgaaggtact catcgcttta gcgacctgcg gcgcaaaatc ggcggggtga
     6541 gcgaaaagat gcttgcgcag tcgttacagg cgttagaaca ggatggtttt cttaaccgta
     6601 tcgcgtatcc ggtggtgcca ccgcatgttg aatacagcct cacgccgctg ggcgagcagg
     6661 tgagcgaaaa ggttgctgca ctggcggact ggattgagtt gaatttgccc gaggtgttgg
     6721 cggtgcggga tgaacgtgcg gcataactgc cggatgcgcg gcctacggtg tatgacttgt
     6781 agggcggata agatgcgcca gcatcgcatc cgccaataaa tgccggatgc gattgatgat
     6841 ttataggccg aaaaagaagt gttttactta ctcaaatcca cctgataaat cgcaaacccg
     6901 atatcatcgg tcgcgacttt attcatcgga tactgccctt tctctttaat aaacgctgcg
     6961 gctttatctg acggggaagt ttcgaaacgg atatccagtt tcttatcggc agctatcggt
     7021 gctaaacgcc agttgttatc tgccgccggg tgaatttccc ccgcacgttt cgactcatca
     7081 gcaatccacg ctgccagcac cgagcggttc tcatccggtg aagcaaaagc gatatggctg
     7141 tcgcccgtac cggcaaattt gccgccgtaa gcgcgatagt tattggtggc aacgaggaac
     7201 atggcgttcg gatcaatcgg cttgccatta aaggtcaggt tcttaatcct ttccgcattg
     7261 gtattaatca tctggcactc gccgtcataa cgggcgggct gggtaacatc aatctgataa
     7321 ttcacaccat caataacatc aaagttataa gtgcggaaac catcccagtt gatgagtgac
     7381 tgtggtttcg tgctgttagg atcaatctgg ttaaactgtc cggcggagca ctccagccac
     7441 tctttcacct ctttaccgct ggctttcact acaatcagcg tattggggta gagataaaga
     7501 tcggcggcat tacggaaggt caactggcct ttttccacct ccacatagct tgccgggtca
     7561 tttttgcgac caccaacttt aaacggtgcg gcagctgaaa gcaccggcag ttttgccaga
     7621 tccggatcgc cctgaatgta atgttcgaca tacgcttttt gcgcgttgtt cacgacttgc
     7681 acggtcggat cgtcctgcac cagcgccaga tagctataca tattgtcggc agatttaccg
     7741 attggcttgc tgacgaactg gcgtgtggca tcgtgatcgg ctttgagtgt ttctaccagc
     7801 ttgctgtctt ccgccgcgag ggatttttta ttggcgatgt cgtaaatcgg ccgagcttcc
     7861 gctttcgcct gcgtcacctg ccatttaccg ctgtcattac tgagttgtaa gtcgaccacc
     7921 ccaagatgat cgccccacat gcccggcatt accgccggaa caccattcag cgtgcctttg
     7981 gtgatatcag ccccttcgat atcagcaaaa tctttacccg ggaaaacggc gtgagcatga
     8041 ccaaacataa tggcgttaac gcccggaatt tcactgaggt aataaactga gttttccgcc
     8101 atcactttat acggatcggc agacagcccg gaatgtgcca gaacgacaac gacatcggca
     8161 cctttctcgc gcatttcagg cacgtatttg cgcacggttt cggtaatatc attcaccgtc
     8221 actttcccgg ataaattagc tttatcccag cccatgattt gcggcggcac gacgccaata
     8281 tagccaatct tcagcgtctg tttttttccg tctttatcga ccacttcggt atctttaatt
     8341 aaatacggtg taaacattgg ctgtttggtt ctggcgtcaa tgacgttggc atttacataa
     8401 gggaatttcg ctcccgccag cgcatttttc aggtaatcca gaccgtagtt aaattcatga
     8461 ttgccgagtg taccgaccgt ataatccagc gtattcagcg ccttataaac cggatgaaca
     8521 tcacctgttt ttaatccttt cgccgacatg taatctgcca gcggactccc ctgaatcaaa
     8581 tcgccgttat cgaccagtac gctgtttttc acttcattgc gggcatcgtt aatcaggctt
     8641 gccgtacgta ccagtccgaa tttttccgtg gcggtgtctt tgtaataatc gaaatccatc
     8701 atgttgctat gcagatcagt ggtttccatg atacgcagat cgaccgtcgc tgcattcaca
     8761 ctggcggcaa tcagcgtggc caggagcgtt gcgctaaact taatcatcag ggacatcctt
     8821 ttatcatcgg gaatacgaaa gaaaagggag aataaacgtc ttacttatag aacagtgaag
     8881 aatgccacaa ttttacgctt tgaaaatgat gacactatca cagttggcgc attcattaac
     8941 gatagggtat aagtaaaaca ataagttaac accgctcaca gagacgaggt ggagaaatgt
     9001 tagatcaagt atgccagctt gcacggaatg caggcgatgc cattatgcag gtctacgacg
     9061 ggacgaaacc gatggacgtc gtcagcaaag cggacaattc tccggtaacg gcagcggata
     9121 ttgccgcaca caccgttatc atggacggtt tacgtacgct gacaccggat attccggtcc
     9181 tttctgaaga agatcctccc ggttgggaag tccgtcagca ctggcagcgt tactggctgg
     9241 tagacccgct ggatggtact aaagagttta ttaaacgtaa tggcgaattc accgttaaca
     9301 ttgcgctcat tgaccatggc aaaccgattt taggtgtggt gtatgcgccg gtaatgaacg
     9361 taatgtacag cgcggcagaa ggcaaggcct ggaaagaaga gtgcggtgtg cgcaagcaga
     9421 ttcaggtccg cgatgcgcgc ccgccgctgg tggttatcag ccgttcccat gcagatgcgg
     9481 agctgaaaga gtatctgcaa cagcttggcg aacatcagac cacgtccatc ggctcttcgc
     9541 tgaaattctg cctggtggcg gaaggacagg cgcagctgta cccgcgcttc ggaccaacga
     9601 atatttggga caccgccgct ggacatgctg tggctgcagc tgccggagcg cacgttcacg
     9661 actggcaggg taaaccgctg gattacactc cgcgcgagtc gttcctgaat ccggggttca
     9721 gagtgtctat ttactaaatt cagatggcag aaacagtgta tttcctgatt ctgccatcct
     9781 gatttctccc aacctaaaaa gttataaata a
//
LOCUS       DADMKF010000143         9720 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.146, whole genome shotgun sequence.
ACCESSION   DADMKF010000143 DADMKF010000000
VERSION     DADMKF010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9720
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.146"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            310..2199
                     /locus_tag="JNC24_003272"
                     /pseudo
     CDS             310..2199
                     /locus_tag="JNC24_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352717.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2192..3328
                     /locus_tag="JNC24_003273"
     CDS             2192..3328
                     /locus_tag="JNC24_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAY9159953.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSGGLGGSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDMLERMQPSESLLRAVLHTKHLMNPEVLAAARRIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSFIPLARNFDFKSTLRANLQHWHPQHGKLYIESPRFNSRIKRQSEQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAVEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDTAQALVNVGAQIAAMTPGELASWLAENLQS"
     gene            3325..5325
                     /locus_tag="JNC24_003274"
     CDS             3325..5325
                     /locus_tag="JNC24_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9159954.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     DALIATFSDGVRTQLANGQALKEAQCSCGANGMCRHRVMLVLSYQRLCATTQSTEKEE
                     EWDPAIWLEELATLPDATRKRAQALVAKGITIELFCAPGEIPSARLPMSDMRFYSRSS
                     IRFARCDCIESTLCEHVVLAVQAFVQAKAQQAELTHLIWQMRSEHVTSSDDPFASEEG
                     KTCRQYVQQLSQALWLSGISQPLIHYEAAFSRAQQAAERCSWRWVSESLRQLRASVDA
                     FHARASHYHAGECLRQLAALNSRLNCAQEMARRDSIGEVPPVPWRTVVGSGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQENSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPLRQPGIVALR
                     EYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNLLTLSL
                     PASACSPFAVERMAALLQQTDDPVSLVSGFVSFVEGQLTLEPRVMMTKTRAWALDAET
                     APVAPLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAISQAELLANDLSAV
                     GFYRLAHVLGQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
     gene            5447..5908
                     /locus_tag="JNC24_003275"
     CDS             5447..5908
                     /locus_tag="JNC24_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HAY9159955.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAAKTLEPLSAKYKNIAGVEEKLTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
     gene            complement(5949..6419)
                     /locus_tag="JNC24_003276"
     CDS             complement(5949..6419)
                     /locus_tag="JNC24_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HAY9159956.1"
                     /translation="MLSNDILRSVRYILKANNNDLVRILALGNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKS"
     gene            complement(6466..7185)
                     /gene="yehT"
                     /locus_tag="JNC24_003277"
     CDS             complement(6466..7185)
                     /gene="yehT"
                     /locus_tag="JNC24_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HAY9159957.1"
                     /translation="MIKVLIVDDEPLARENLRVFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(7182..8867)
                     /gene="btsS"
                     /locus_tag="JNC24_003278"
     CDS             complement(7182..8867)
                     /gene="btsS"
                     /locus_tag="JNC24_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HAY9159958.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISSP
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
     gene            9089..9154
                     /locus_tag="JNC24_003279"
                     /pseudo
     CDS             9089..9154
                     /locus_tag="JNC24_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
     gene            9382..9630
                     /locus_tag="JNC24_003280"
     CDS             9382..9630
                     /locus_tag="JNC24_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI family protein"
                     /protein_id="HAY9159959.1"
                     /translation="MRVEICIAKEKITKMPNGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVR"
     gene            complement(9641..>9720)
                     /locus_tag="JNC24_003281"
     CDS             complement(9641..>9720)
                     /locus_tag="JNC24_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HAY9159960.1"
                     /translation="SLSFSKSVELHDKVIGHYLNIKHYQ"
BASE COUNT         2379 a         2552 c         2489 g         2300 t
ORIGIN      
        1 cggccacgat acgtattgat tgaaggcccg gctgatttta atgaccgggt agacgaactg
       61 tttttagccc accagcttcc ggtagctatt tacagttatt gccagtatca ggacggtgca
      121 gcccccgggc gtggtgcctg gacgccattt gctgaatttt cgccggagtg gcaggcgcta
      181 caagccgcac gtcgtattca ggcacaaact tacttcatcg atttgccttg ctgggcgcag
      241 agtgaagaag tggacgattc gcctgatacg caagaggaaa gccaggcctt gctgctgcgt
      301 gccacccgca tggataacag cgacaccctg tgggatcact tgttcgaaga tgaaagccag
      361 caaactgcat taccctctgc actggcgcac tattttgccc aactgcgggg cgattccccc
      421 ggcgatgcac tcaatcgtca gcgcgaagcc tttatggccc gctggattgg atgggcgatg
      481 cagcaaaaca atggcgacgt gttagtcgtc tgcggtggct ggcacgctcc tgcactggca
      541 aaaatgtgac gcgaatgccc gcaggacatt aacacgccgg aattgccctc gctggcagat
      601 gccgttacag gttgttatct caccccctac agtgaaaagc gccttgatgt gctggcagga
      661 tacctttccg gtatgcctgc cccggtctgg caaagctggt actggcagtg gggcttacag
      721 caggcgggag aacaactgct aaaaacggtt cttacccgtt tgcgccagca caaattgcct
      781 gcttcgaccg cggatatggc tgccgctcat ctgcatgcga tggcgctggc acagttgcgc
      841 ggacataaac taccgttacg cactgactgg ctggatgcca tagcaggctc gctgattaaa
      901 gaagccctga acgcgccgtt gccgtggagc tatcgcggag ttattcatcc cgacaccgat
      961 ccgattctgc taacgttgat agacacatta gcgggtgacg gattcggtaa acttgcccct
     1021 tctacgccac aaccgcctct gccaaaagat gtcacctgcg aactggaacg taccgcaatc
     1081 tctcttccgg cggagcttac cttaaatcgc tttaacccca atgggctagc gcaaagtcag
     1141 gtgttacatc ggctggcaat actggagatc ccagggattg tacgccagca gggaagcaca
     1201 ctgacacttg caggcaacgg tgaagaacgc tggaaattaa cccgcccgct tagccagcat
     1261 gcggcattga ttgaggccgc ctgctttggt gccacactcc aggaagccgc acgccataaa
     1321 ttagaagccg atatgctgga cgcgggtgga atcggcagta tcaccacatg tcttagccag
     1381 gcggcgttag cgggtctggc gtccttcagt caacaattac tggagcaact cacattatta
     1441 atcgcccagg aaaatcaatt tgccgaaatg ggccaggcgc tggaagtgct atatgcctta
     1501 tggcggctgg atgaaattag cggtatgcaa ggcgcgcaga tattacaaac gacgttatgc
     1561 gcggctatcg atcgcacgct gtggctgtgt gaatctaacg gcagaccaga tgaaaaggag
     1621 tttcacgctc acctgcatag ctggcaggcg ctttgccata tcctgcgcga tctacatagc
     1681 ggcgttaatt tacccggcgt ttttctttct gcggcggtag ccttactgga gcgccgcagt
     1741 caggcgattc atgtcccggc gctggatcgc ggcgcggctc ttggcgcact gatgcgtctg
     1801 gaacatccca acgccagtgc cgaagcggcg ctgacgatgc tggcgcagtt atccccggca
     1861 caatccggcg aggcgctgca cggtttgctg gcgctcgccc gtcatcaact ggcctgccaa
     1921 ccagcattta tcgccggttt cagtagtcat ttaaatcaac tgagtgacga cgattttatt
     1981 aacgccctgc ccgatttgcg cgcggcgatg gcctggctac cgccacgaga acgcgggaca
     2041 ctggcgcatc aggtgcttga gcattaccag cttgcgcagc ttcctgtttc agcgctgcaa
     2101 atgccgttgc attgtcctcc gcaagccatt gcacatcatc aacaactcga acagcaggca
     2161 ctggcatcgc tgcaaaactg gggagttttc catgtctgaa ctgaacgatc ttctgaccac
     2221 ccgtgagcta caacgctggc gattaattct tggcgaagcg gcagaaacga cgctttgtgg
     2281 gctggatgac aacgcccggc agatagacca cgcgctggaa tggctgtatg ggcgcgatcc
     2341 tgaacggctc cagcgtggtg aacgctccgg tggattaggt ggctcaaatc tcaccactcc
     2401 tgagtggatc aacagtattc acacgctgtt tccgcaacag gtgattgagc ggctggaaag
     2461 cgatgccgta ctgcgctacg gcattgaaga tgtggtgacg aatctcgaca tgctggaacg
     2521 tatgcagcct tctgaaagcc tgctacgcgc cgttttgcac accaaacatc tgatgaatcc
     2581 cgaagtactg gctgccgccc gccggatagt gcgccaggtt gttgaagaaa ttatggctcg
     2641 actggcaaag gaagttcgtc aggctttttc tggtgtccgc gatcgccgtc gccgctcatt
     2701 tattccactg gcgcgaaact ttgatttcaa aagtactctg cgcgctaacc tgcaacactg
     2761 gcacccgcaa cacggcaagt tgtatatcga atccccccgc tttaacagcc gcattaaacg
     2821 ccaaagcgaa caatggcaac tggtcttact ggttgatcaa agcggatcga tggtggactc
     2881 ggtgatccac tctgcggtga tggcggcctg tttgtggcag ttacccggca ttcgtaccca
     2941 tctggtggcg tttgacacaa gcgtggttga tctcacggca gacgttgccg atccggtaga
     3001 gttattaatg aaagtacagt tgggcggcgg gaccaatatc gccagtgccg tggagtatgg
     3061 tcggcaactt attgaacaac cagcaaaaag cgtcattatc ctcgtgagcg atttttacga
     3121 agggggttca tcatcattgc tgacgcatca ggtgaaaaag tgtgtccaga gcggcatcaa
     3181 agtgctggga ctggctgcgc tcgatagcac cgcaacacct tgctatgacc gcgatacggc
     3241 ccaggcgctg gttaatgtcg gcgcacaaat agccgccatg acgccgggcg agctggcatc
     3301 atggcttgcg gagaatcttc agtcatgaat tcactacgtc cggaattatt agaactgaca
     3361 ccgcaggccc tgacggcgtt aagcaatgcc ggttttgtta agcgcagtct taaggaactg
     3421 gaaaatggca acgtcccgga gatcagccat gagaacgacg ctttaatcgc caccttcagt
     3481 gacggtgtcc gtacccagct ggcgaacggc caggcactga aagaggctca gtgcagttgc
     3541 ggggccaacg gtatgtgccg tcatcgcgtg atgctggtgt taagttatca acgactttgt
     3601 gccaccactc agtctacgga aaaagaagaa gagtgggatc cggcaatctg gctggaagaa
     3661 ctggctaccc ttcccgatgc tacccgcaaa cgcgcacagg cgctggtcgc taaaggcatc
     3721 accattgagt tgttctgtgc gccgggtgaa attccctctg cccgcttacc gatgagcgat
     3781 atgcgttttt attcccgcag cagtattcgt ttcgcccgtt gtgattgtat tgaaagcaca
     3841 ctttgcgaac atgtcgtact ggcagtacag gcctttgtcc aggcaaaagc gcagcaagcg
     3901 gaattgactc atttaatctg gcagatgcgc agcgaacacg tcacatcatc tgacgatccg
     3961 tttgccagcg aagaaggcaa aacgtgtcgt caatatgttc aacaactaag ccaggcatta
     4021 tggctgagcg gcatcagcca gccgcttatt cactacgagg ccgctttcag ccgcgcgcag
     4081 caagcagcgg aacgctgcag ctggcgatgg gtgagtgaat cgctacggca actgcgcgcc
     4141 agcgttgatg ccttccacgc ccgcgccagc cactatcatg ccggagaatg cttacgtcag
     4201 cttgcggcat taaacagtcg attaaattgc gcacaagaga tggcccggcg cgacagtatt
     4261 ggtgaagttc ctcctgtgcc gtggcgcacg gtcgttggct ctggcattgc cggagaagca
     4321 aagcttgatc atctgcggct ggtgtcttta ggtatgcgtt gctggcagga tattgagcat
     4381 tatggtttac gcatctggtt taccgatccc gacaccggca gtattttgca cctttcgcgc
     4441 agttggccgc gaagtgaaca ggaaaactca ccggcagcta cgcgtcggct gtttagtttt
     4501 caggctggcg cactagcggg cgggcaaatt gtttcacaag cagcaaaacg cagtgccgat
     4561 ggcgagctgc tgttagctac ccgcaaccgc ttaagcagcg ttgtgccgct gtcgcctgat
     4621 gcctggcaaa tgttgagcgc gccgttacgc cagccgggca ttgtggcttt gcgggaatat
     4681 ttacgccagc gtccccccgc ctgcatacgg cctcttaatc aggtcgataa cttatttatt
     4741 ctgccggtcg ctgagtgtat ttcgctcggt tgggatagca gccgccagac gctggatgcg
     4801 caggtaatca gcggcgaagg tgaagataat ctcctgacgt tatcccttcc ggcatctgcc
     4861 tgttctcctt ttgccgttga acgcatggcg gcgcttttgc aacaaacaga cgaccccgtg
     4921 agtctggttt ctggctttgt cagctttgta gaggggcaat tgacactgga accacgggtg
     4981 atgatgacaa aaacccgcgc ctgggcgctg gacgcagaaa ctgcgcctgt ggcaccgcta
     5041 ccttctgcca gcgttttgcc tgtgccgtct accgctcatc agttgctgat gcgctgccag
     5101 gcgttactta ttcaactgct ccataacggc tggcgctatc aggaacagag cgctattagt
     5161 caggcagagt tgctggcgaa tgacctctcc gcggtcggtt tttatcggct ggcacatgtg
     5221 ttgggacaat ttcgtaatac agaaagcgag gcacgggtag aagcaatgaa taacggtgtt
     5281 ttactttgcg aacaattatt ccccatgctt cagcaacaag gatgaaatag tgcttactaa
     5341 gagttctact ccagttccgg actgctcacg ccacggtatt aggcatatcc tatatagccc
     5401 ctggtgagag tcaccagttc cttgattaaa taaaatggag ttttacatga aggctttcaa
     5461 taagctgttt tccctcgttg ttgcatctgt tctggttttc tctcttgctg gctgcggtga
     5521 caaagaagaa tcgaagaaat tcagcgccaa tctgaacggc actgaaattg ccattaccta
     5581 tgtctacaaa ggtgacaagg tgcttaagca atcttctgaa accaaaattc aatttgcctc
     5641 cattggtgca accaccaaag aagatgctgc caagacactt gagccgttaa gcgccaaata
     5701 caaaaacatc gcgggtgttg aagaaaaatt aacctatacc gatacctacg cgcaggaaaa
     5761 cgtgactatc gatatggaaa aagtggattt taaagccctg cagggtattt caggaatcaa
     5821 cgtttctgct gaagatgcca aaaaaggtat cactatggcg caaatggaac tggtgatgaa
     5881 agccgctggt tttaaagaag tgaaataatc tgtcggcggc catgtttcgc atggccgcca
     5941 tacccgtctt agcttttctt cacatgctgg cgcgctgcca gtccacgcag aaaataacgt
     6001 aaaaattgat cgccgcattc gcggaagttt ttatgatccg gtgcgcgcat catcgctgta
     6061 atttccggca tcgaaacgcg gaactgctgt tcggtgagga tcgccagaat gtcatcggtt
     6121 ttcagcgaaa acgcgatgcg taattttttc agcacgatgt tgttattaat gcgacgttcc
     6181 ggctccagtg ccggagcaga ctcatccttg ccgcgttttt cataaatcag gccattgagg
     6241 aatgacgaca aaacaatgtc cggacaacgc tgaaaaccct cttcgtcttc tttacgtagc
     6301 cagacggcga tctgttccgc ggtggcttcg acattaccca gcgccagaat acgcaccagg
     6361 tcattattat tggctttcaa aatgtagcgc acgctgcgca gaatatcgtt acttagcatg
     6421 aggccttcag gtgttaatga ggcaaaaagc cattttagca gtcttttaca ggccaatcgc
     6481 ctcttttaag cttttcagat aacggcggct gaccggcacg gttaagccat tacgcaaaat
     6541 caactcggcc tggccgttat cttccagacg aatctcctgt aaatgcgcga ggttaaccag
     6601 atactgacga tggcagcgca gtagtggtgt acgactttcc agggtacgta atgtcaattc
     6661 ggtaaagccc tctttccctt cgtggctggt aacgtagaca ccgctcatcc gactgctgac
     6721 aaatgccaca tctttcattt gcagcaaata aatccgacta tgccccgtac aagggataaa
     6781 tttcagcgcc tgttgatttt ccggtaacag cgaaacatcc tgcttgctgc gctcctgacg
     6841 caatcgcgcc agcgttttct ccagtcgcgc ttcatcaatt ggcttcagca gataatcaaa
     6901 ggcatgttct tcaaaggctt taatagcgta ttcgtcaaac gcagtgagaa aaacaatata
     6961 cgggcgatgt tccgggtcaa gcatccccac catttccaga ccactgatgc gcggcatctg
     7021 gatatcgaga aacagcacat ccgggcgcag tttatgcacc gcgccgatcc cttccacggc
     7081 gtttgaacac tctccaacga tttcaatatc gctctgctcc tgcaaaaata cacgcaggtt
     7141 ctcccgtgct aacggttcat catcgacaat taagacttta atcatgcctc gtccctccat
     7201 ggtagtcgta acgttattcg ggtgtaacta tcaggctcac aggcgacgct tattccatag
     7261 tcatcgccaa accgttcacg taaacgctta tccaccagat tcatccccag cccactggca
     7321 ttggttaccg gttgatacaa accggcattg tcttcgatct ccagcatcaa atgttgcccc
     7381 tcacgtcggg cgctgattgc cactcgccct gtatccagca gttgtgatgt cccatgttta
     7441 atggcgtttt ccactatcgg ttgcagggta aacgcgggca attgctgctg ggataattct
     7501 tgcggaatag caatgttgac ctgcaaccgc gactggaagc gcgccttttc aatttgcaga
     7561 taagcgttca catgttcaat ttcgtcggcg agagtaacaa actccgaagg ccgctttaag
     7621 tttttgcgga aaaaagtgga aagatactgc accagctggc tggcctgttc gctgtcgcgg
     7681 cggatcaccg ctttaatggt gttaagcgca ttaaacaaaa aatgaggatt cacctgggcg
     7741 tgaagcagtt tgatttctga ctgggtgagc atcgcttttt gccgctcata ttgcccggca
     7801 agaatctgcg ccgaaagcag ttgcgcaatc ccctcgccca gcgtgcggtt tattgaactg
     7861 aataaacggt ttttggcttc atacaatttg atggtgccca tcacgcgctg attttcgcca
     7921 cgcaacggaa ttaccagcgt cgaccccagt ttgcattgcg gatgcaaaga gcaacgataa
     7981 ggtacttcgt tgccatcagc gtagaccact tcaccggttt caatcgcttt taaggtgtaa
     8041 ggcgaagaaa tcggtttgcc gggtaaatgg tggtcgtcac caattccggt aaaggccagc
     8101 aatttctctc gatcggtaat cgcgactgca ccaatatcca gctcctgata cagcacctga
     8161 gccactttca tgctgttcac ttcgttaaac ccctgtcgca aaatgccttc cgtcgaggct
     8221 gccactttca gcgcagtggc agaaaaagcc gaagtgtatt tttcaaacat cgcgcgttta
     8281 tcgagcaata tacgcataaa cagcgccgcg ccgacggtat tggtgaccat cattggcgca
     8341 gcaatattac tcaccagacg caccgcatct tcataaggtc gggcgatcgc caggatgatc
     8401 agcatttgca ccatttcagc gacgaacgtg acggcaccgg cggtaatggg gttaaagact
     8461 ttatcagtgc gcccgcgacg gatcaggatg ctgtgtacca ggccgccgag caatccttca
     8521 acgatggttg agatcatgca acttagcgcg gtcatgcccc ccatcgaata tcgatgtaag
     8581 ccgccggtca gaccaaccag cccaccgacg accggaccgc cgagtaagcc gcccattacc
     8641 gcgcctatcg cacgggtatt ggcaatagaa tcgtcaatgt gcaacccaaa ccaggtgccc
     8701 atgatgcaga agatggaaaa gacgatgtag cagagaaatt tatgcggcag acgaaccgtg
     8761 acctgcatta acggtatgaa taatggcgtt ttactcatta accacgcaat gactaaaaaa
     8821 acgcacatct gctgaagcag cagcaacacc agattaaaat cgtacatacc cgcaaaccac
     8881 acttcccttt aaaacgcgta acatacattg cctgcgttta actttctttg aactcttgca
     8941 gaaaaatgag aattcgtgag tacgatcact caaaatcgcc tggcaaaaat aaaatcaccc
     9001 tatagatgca caaaaaacgg gcaaaactac ctggttcgca aaactgcgtc taaagttaaa
     9061 ccgggacctc gcgagcaagg gtgagacgat ggcgctttac acaattggtg aagtggcgtt
     9121 gctttgtgat attaaccctg tcacgttacg cgcgtgacgc aacattttta aaaatcgacg
     9181 aagatcgtcc aggagcgccg tttttcaagg gttggataga tgattcgttt accatgtcga
     9241 cgaacttcga cgaatttcgc cgataaaaag tgtcccatac atgccccatt gatgccccat
     9301 aaacaatatc aggaattgac actggttatc catacagata aaaataacac ctgtacataa
     9361 ccacagttac aggagggtgt tatgcgtgtt gagatctgta tcgctaaaga aaaaatcact
     9421 aaaatgccaa acggtgctgt ggatgcgtta aaggaagaat taacccgacg catcagtaaa
     9481 cgttatgacg atgtagaggt gatcgtaaaa gccaccagca acgatggcct ttctgttaca
     9541 cgcaccgcag ataaggattc tgcaaaaaca tttgttcagg agactctgaa agatacctgg
     9601 gagtctgctg acgagtggtt tgttcggtaa tgactccaac ttattgatag tgttttatgt
     9661 tcagataatg cccgatgact ttgtcatgca gctccaccga ttttgagaac gacagcgact
//
LOCUS       DADMKF010000144         9644 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.147, whole genome shotgun sequence.
ACCESSION   DADMKF010000144 DADMKF010000000
VERSION     DADMKF010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9644
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.147"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(<1..667)
                     /locus_tag="JNC24_003282"
     CDS             complement(<1..667)
                     /locus_tag="JNC24_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAY9159961.1"
                     /translation="MNITPFPTLSPATIDAINVIGQWLAQDDFSGEVPYQADCVILAG
                     NAVMPTIDAACKIARDQQIPLLISGGIGHSTTFLYSAIAQHPHYNTIRTTGRAEATIQ
                     ADIAHQFWHIPHEKIWIEDQSTNCGENARFSIALLNQAVERVHTAIVVQDPTMQRRTM
                     ATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSVIFINQLQGLWPVERYLSLLTGELPR
                     LRDD"
     gene            complement(939..4841)
                     /gene="hrpA"
                     /locus_tag="JNC24_003283"
     CDS             complement(939..4841)
                     /gene="hrpA"
                     /locus_tag="JNC24_003283"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HAY9159962.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHGVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DSALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKIRKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQVMWNGLRRLLLLNIPSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDKLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
     gene            5042..5647
                     /gene="azoR"
                     /locus_tag="JNC24_003284"
     CDS             5042..5647
                     /gene="azoR"
                     /locus_tag="JNC24_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HAY9159963.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWREKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            complement(5698..7014)
                     /locus_tag="JNC24_003285"
     CDS             complement(5698..7014)
                     /locus_tag="JNC24_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2/dual specificity phosphatase
                     family protein"
                     /protein_id="HAY9159964.1"
                     /translation="MITERRNVLLQGAGWLLLLAPFFFFTYGSLNQFTAVQDSNSHDI
                     PSQVFGWETAIPFLPWTIVPYWSLDLLYGFSLFVCSTTFEQRRLVHRLILATVMACCG
                     FLLYPLKFSFIRPEVSGVTGWLFSQLELFDLPYNQSPSLHIILCWLLWRHFRQHLAVR
                     WRKVCGGWFLLIAISTLTTWQHHFIDVITGLAVGMLIDWMVPVDRRWNYQKPDQRRIK
                     IALPYVVGACACIVLMELMMMVQLWWSVWLCWPVLSLLIIGRGYGGLGAITTGKDSQG
                     KLPPAVYWLTLPWRIGMWLSMRWFCRRLEPVSKMTAGVYLGAFPRHIPAQNAVLDVTF
                     EFPRGRATKDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCALGLSRSA
                     LVVAAWLLCYGYCKTVDEAISFIRARRSHIVLKEDHKAMLKLWENR"
     gene            complement(7004..8761)
                     /locus_tag="JNC24_003286"
     CDS             complement(7004..8761)
                     /locus_tag="JNC24_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HAY9159965.1"
                     /translation="MENSRISGEHFFTTSDNTALFYRHWPALQPGAKKVIVLFHRGHE
                     HSGRLQHIVDELAMPDTAFYAWDARGHGKTSGPRGYSPSLARSVQDVDEFVRFAASDS
                     QVGLEEVVVIAQSVGAVLVATWVHDYAPAIRGLVLASPAFKVKLYVPLARPALTLWHR
                     LRGLFFINSYVKRRYLTHDRQRVASFNNDPLITRAIAVNILLDLYKTSERIIRDAAAI
                     TLPTQLLISGDDYVVHRQPQIDFYQRLRSPLKELHLLPGFYHDTLGEENRAQAFEKMQ
                     SFIRRLYANKSQKFDYQHEDRTGPSADRWRLLSGGPVPLSPVDLAYRFMRKAMKLFGT
                     HSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRLIDKIYLNSVGWRGIRQRKTHLQ
                     ILIKQAVADLHVKGLAVRVVDIAAGHGRYVLDALANEPAVSDILLRDYSELNVAQGQE
                     MIAQRGMSGRVRFEQGDAFNPEELSALTPRPTLAIVSGLYELFPENEQVKNSLAGLAN
                     AIEPGGILIYTGQPWHPQLELIAGVLTSHKDGKLWVMRVRTQGEMDLLVHDAGFDKCT
                     QRIDEWGIFTVSMAVRRDN"
     gene            complement(8777..9640)
                     /locus_tag="JNC24_003287"
                     /pseudo
     CDS             complement(8777..9640)
                     /locus_tag="JNC24_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005142702.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
BASE COUNT         2448 a         2627 c         2273 g         2296 t
ORIGIN      
        1 tatcatcgcg taaacgcggc agctcgccag tgagtagtga gagataacgc tcaaccggcc
       61 ataatccttg taactgatta ataaagatta cactgtctgc gttatttcct aactgaggaa
      121 cgaatccggg ataacttaac cagcgtggtg catcgggatt gtccccagtc atacggcgga
      181 acgtcgccat cgtgcgccgc tgcatggtgg ggtcctgaac aacgatagcc gtatgaactc
      241 gttctacggc ctgattcaat agcgcgatgc taaagcgtgc gttttcaccg cagtttgttg
      301 actggtcttc aatccagatt ttttcatgcg gaatgtgcca gaactgatga gcgatatccg
      361 cctggatggt cgcttctgct ctgccagtgg tgcggatagt gttgtagtgc ggatgctgtg
      421 cgatggcgct atacaaaaaa gttgtcgagt gaccgatacc accactaatc agtaaaggaa
      481 tttgctgatc gcgagcaatc ttacatgccg cgtcgatagt cggcataacc gcattgcctg
      541 caaggatcac gcaatcggcc tgatacggca cctcaccgga gaaatcatcc tgcgccagcc
      601 actgtccgat aacatttatg gcatctatag ttgccggcga aagcgtcgga aacggggtaa
      661 tgttcatggt tcctccttat cgttccctta agaatactgc gtgagataat agtgagaagc
      721 ggagcactct gaaaggagga agtcgtattt ccttaaccgc cactccgcaa gcctgacata
      781 tcttttaagg aaatgctgta gataaacatt attcatcttg cagcagagaa ttgtattttt
      841 ctgaattcct gaatttttac ccttaccgtt ttagaattaa taaaaaaccc ggcgctaaat
      901 gcttaccggg ttttttcttt atcaggcaaa tagcagggtt aaccgctaat ctgctccatc
      961 gcctgcaaaa tacgcttatc tgaaatcgga taaggcgtac caagttgttg agcgaagtaa
     1021 ctaacacgca actcttctat catccaacgg atctctttca cgtcttcatc ctcacgacgt
     1081 gcaggcggca gtttgttgat ccattgctgc cacgcctgct ggacgttttc gactttcagc
     1141 atctgagcac ggtcgcgatg tggatcaacc gccagttttt ccagccgttt ttcaatcgcc
     1201 tgcaaatatc gcagcgtgtc gcccagccgt ttgaagccgt taccagtgac aaaaccgcga
     1261 tataccagcc cgcccatctg cgctttaatg tcagaaagcc ccagcgccat cgtcatatcc
     1321 acccgcccct tcagacgttt gttgatattg aacactgccg taaggatttg ctcgacctgc
     1381 ttcgcaatat ccaccaccgt gtcgttcagt tcggcacgca ctttttcatg cagcgcagca
     1441 aagccttctt ccgtccagac cgggccacca ttggcgtcga tcaatttatc cacaccgcag
     1501 gagatacagt cgtcgatcag ctccagcact ttgccatacg ggttaaagta cagccccagc
     1561 ttggctttgt ttggtaactt ctcatgtaaa tacttgatcg gcgagggaat attcagcagc
     1621 agtagacggc gaagaccgtt ccacattacc tgtttttgct ccagcggatt atcaaacagt
     1681 ttgatcgcca cactgtcgcg ttcatccact agcgctggcc acgccttcac tttgtagttg
     1741 ccacgcttct gttcgtagct ttccggcagc tgaccaaaac tccagatatg cagtccgctc
     1801 tgctcgatac cgtcatccgc caccgcagac agcgtttcct gcactttgcc tttcagcgca
     1861 tctttcagat cttgtagcga gcgcccttct tttagcttct tgtttttgtc atccaccacg
     1921 cggaaggtga ttttcaggtg atcgggcacc tgatcccagt gccagtcttc gcggtcaacg
     1981 gtaacgccgg tcatccgccg taactcgcgc tcaaggctgt cgagcaacgg taactccagc
     2041 ggtgtgacgc ggcctaaaaa cgcttcggcg tagtttggtg caggcacaaa attacggcgt
     2101 accggtttcg gcaacgattt aatcagagca atcaccaatt cgcggcgcag accggggatc
     2161 tgccattcaa acccgctttc ctcaacctgg ttaagtaacg gcagcggaat atgtacggtc
     2221 acaccgtccg cgtccgcgcc tggctcaaac tgatagctca aacgcagctt gagattgccc
     2281 tgatgccaga agttcgggta atccagcttt ctgatttttt ccgccccttc tttgatcaac
     2341 atgctctttt caaagttgag caaatcaggc gtttcgcggc tgactttttt ccaccagcta
     2401 tcgaagtgac gagcggagat tacatcgtgg ctgatgcgct ggtcgtagaa ctcaaacagc
     2461 gtttcgtcat caaccagaat atcgcggcga cgtgatttgt gttccagctc ttccacttcg
     2521 gcccgcagtt tcaggttttc acggaagaat gcgtgacgcg tctgccagtc accttccacc
     2581 agagcgtggc gaataaagag ttcacgacat aacgccgaat cgatctggct gtagttgacc
     2641 ttgcgcgcag caacaatcgg caaaccataa acagtgactt tttccgttgc catcaccgcg
     2701 ccctgcgccc gttcccagtg cggttcgctg taggtgcgtt taatcaaatg ctgagcaacg
     2761 ggttctaccc attccgggtc gatacgcgca gcaatgcgcc cccacaggcg gctggtttct
     2821 accagttccg ccaccattac ccatttcggc ggctttttga ataaaccaga accggggaag
     2881 atggagaaac gcgcgttacg tgcaccggta tattcctgtt tatcggcatc tttcatgccg
     2941 atatgggaaa gcaaaccggt cagtaaggcg atgtgaattt cacgatactc cgccggttcg
     3001 ctgttgaccg gaatgccaag ttctttcacc acctggcgca actgggtgta gatatcctgc
     3061 cattcgcgca cgcgcagata gttgaggtaa tcggtacgac acaggcgacg gaaggcgttg
     3121 gaagaaagcg ccttttgctg ctcgccaaga taattccaca gatttacaaa cgcgaggaag
     3181 tcggattctt tgtcgtggaa gcgacgatgt ttttcgtccg atgcctgctg tttgtccatc
     3241 ggacgttcgc gcggatcctg aatggagagc gcggacgtga taatcatcgc ctcacgcacg
     3301 cagccatgtt tttgcgcttc cagcaccata cgcgccagac gtgggtcgac aggcaactgc
     3361 gagagctggc gaccgagcgg cgtcagttta taggcgctgg cctgttcatc cgtggtgatc
     3421 gcgcccagtt cttcgagcag acgcacgcca tcctggatat tgcgtttatc cggtgcttcg
     3481 acaaacggga acgcagcgat atcgcccagc cccagcgcgg tcatctgcaa aataaccgag
     3541 gccaggttgg tacgcagaat ctccggatcg gtaaactccg ggcgcgagag gaaatcgtct
     3601 tcggaataga gacgaataca gatcccttcg gacacacgac cacagcggcc tttacgctga
     3661 ttggcagacg cctgcgaaat gggttcaatc ggcaaacgct gcactttggt gcgatagctg
     3721 tagcggctga tacgcgctgt accggggtcg atgacgtatt taatccccgg tacggtcagc
     3781 gaggtttccg cgacgttggt cgccagcaca atgcgccgtc cgctgtgcga ctggaacacg
     3841 cggttctgtt cgctgttcga aagccgcgca taaagcggca agatttcggt atggcgtaag
     3901 ttcagcttgt tcagcgcatc ggcggtatcg cgaatttccc gctcaccact cataaagatc
     3961 agaatgtcgc cagggctttc ctgactcagt tcgtctacgg cgtcaaaaat cgcctgcaac
     4021 tgatcgcgct cggtgtcatc ggcttcttca acaatcggtc gatagcgcac ttccaccgga
     4081 taggtccgac cggagacttc gataatcggc gcattattaa agtggcgcga aaagcgttcc
     4141 gggtcgatag tcgcggaagt gataatgatt tttaggtcag gacgccgcgg cagcaactct
     4201 ttcaaatagc cgagcagaaa atcgatattc aggctgcgtt cgtgcgcttc gtcaataatg
     4261 atggtgtcgt actgcatcag caggcggtct tgctggatct ccgccagcag gataccgtcg
     4321 gtcatcagct tgaccatcgt gttatcactt acgtgatcgc tgaaacgcac tttgtaaccg
     4381 atgcaaccgc ccggctccgt tttcagctct tccgcaatac ggttcgccac cgttcttgcc
     4441 gccaggcgac gcggctgggt atggccgatc agccctttaa tcccgcgccc cagctccata
     4501 cagattttcg gtaactgagt cgttttacca gaacccgttt ccccggcgac gatcaccacc
     4561 tggtgatcac gaatcgcttc gagaatgtcc tgttttttct gactaaccgg taaattgtca
     4621 ggataagtaa tttccggtcg tgccgcttca cgcagcagga ctttccctgc cgcctggtca
     4681 atctctttcg ccatctcctg gaaaatggcc tgttgtgcat caggattttt aaccttcttc
     4741 acgccgtgca gacggcgaga aaaacgcagt ctgtcacgca gcatcagcga atccagccgc
     4801 tgctgcaagg ccgtaaaggt caatttttgt tgttctgtca taacgttagt gggcagtagt
     4861 ctgcctgatt aattttcttt gaaatgatag atatagagta ccacatcacg gctttctatg
     4921 cgttgttcaa taaattcgaa cataggcttc gatttattgc gctatctctg tggcaggatt
     4981 gtgaataaag tgtcaacaag caacggggca tcgcccattc aaacatctat aaggaaacac
     5041 catgagcaag gtattagttc ttaaatccag catcctggca gggtactctc agtctaatca
     5101 gttgtccgat tattttgttg aacaatggcg cgaaaagcac tccgctgatg aaatcaccgt
     5161 tcgcgacctg gctgcaaatc cgattccggt actggatggc gaactggttg gtgcactgcg
     5221 tccgagcgat gcgccgctga ctccgcgtca gcaggaagct ctggcgcttt ccgatgagct
     5281 gattgccgag ctgaaagccc acgacgttat cgttattgcg gcaccgatgt ataacttcaa
     5341 catctcaact cagttgaaaa actattttga cctggttgca cgcgcaggcg ttactttccg
     5401 ctataccgag aacggtccgg aaggtctggt aacgggtaaa aaagccatcg ttattaccag
     5461 ccgcggtggg atccacaaag atggaccaac ggacctggtg acgccgtatc tgtccacgtt
     5521 cctcggcttt atcggcatta ctgatgtgaa atttgtcttc gccgaaggga tcgcatacgg
     5581 tccggaaatg gcagcgaaag cgcagtctga cgcgaaagca gccatcgaca gcattgttgc
     5641 tgcataaccg gacacgatct tgatgtggac tgtagtccag tccacatccc aatccactta
     5701 cctgttttcc cataatttca gcatcgcttt gtgatcttcc ttaagcacaa tatgcgagcg
     5761 tctggctcga ataaagctaa tcgcttcatc aacggtttta cagtatccgt aacataacag
     5821 ccatgccgcc actaccagcg cactgcgcga taatcccagt gcgcaatgga ccagaacgct
     5881 gccttgctct tcgcgtaatg tttccagcat cgccacggcc tgtcggagct ccccctcttc
     5941 cggaaccacc agatctagca tcggtacaca gaaatagagt cgatcttttg tcgctcgtcc
     6001 acgagggaat tcaaaggtga cgtccagaac cgcattctgt gccggaatat gtcgtggaaa
     6061 cgcccctaaa taaacaccag cagtcatttt gctcaccggc tccaggcgac gacaaaacca
     6121 acgcatagat agccacatcc cgatgcgcca gggcaatgtc agccagtaaa cggcgggcgg
     6181 gagtttcccc tgactatctt tccctgttgt tatcgcgcca agcccaccgt acccacggcc
     6241 aataatgagt agcgataata ctggccaaca taaccagact gaccaccata actgaaccat
     6301 catcattagc tccatcaaca caatgcacgc gcacgcgcct acgacatatg gcagtgctat
     6361 tttgattcga cgttgatcag gtttctgata attccaacga cggtcgacgg gcaccatcca
     6421 gtcaatcaac atacctaccg ccagccctgt gatgacatca ataaaatgat gctgccatgt
     6481 cgtcagcgtc gaaatggcga tgagtaaaaa ccatccgccg cagactttac gccacctcac
     6541 agccagatgc tgacgaaagt gacgccaaag tagccagcag agaataatat gcagcgaagg
     6601 agactggtta taaggcagat caaacagttc aagttgcgaa aatagccatc ccgtcacccc
     6661 actcacttca ggacggataa aactaaactt cagcggatag agcaaaaaac cgcagcaggc
     6721 cattaccgtt gccagaataa gccggtggac aagtcggcgc tgttcgaatg tcgtgctaca
     6781 aacgaacagc gaaaatccat ataaaagatc cagactccag taaggaacaa tagtccaggg
     6841 aagaaaaggg atcgccgttt cccaaccgaa tacctgactg ggaatatcat ggctattact
     6901 gtcctgaacc gcggtgaact gattaagaga tccataggtg aagaagaaaa acggggccag
     6961 caacaataac cagccagctc cttgtagcaa tacgttccgg cgttcagtta tcacgacgca
     7021 ccgccatcga aaccgtaaaa atgccccact catcaatccg ttgtgtgcat ttatcaaatc
     7081 cagcatcatg cacgagcaaa tccatctccc cttgcgtacg cacgcgcatt acccacagtt
     7141 taccatcttt atgactggtt aacaccccgg caatcagctc cagttgtggg tgccacggct
     7201 gcccggtgta gatgagaatg ccacccggtt cgatggcatt ggcaagacct gcgagtgagt
     7261 tttttacctg ctcattttcg ggaaacagct catacagtcc agagacaatc gccagcgtag
     7321 gccgcggagt taacgcgctg agttcctccg ggttaaacgc atcgccctgt tcaaaacgca
     7381 cccgcccaga cattccccgt tgggcaatca tctcttgccc ctgtgcaaca tttaactcac
     7441 tgtaatcacg taataaaata tcgcttacgg caggctcgtt tgccagtgca tccagtacat
     7501 agcgcccatg ccctgcggca atgtcaacca cgcggacggc taaacctttg acgtggagat
     7561 cggcaacggc ttgtttaatc agtatttgta aatgagtttt gcgctggcga ataccgcgcc
     7621 agccaacact gttcaggtaa attttgtcga ttaagcgccc gaatgcgtta ctgccttgcg
     7681 gttgattttg atagacataa tccagcgaac tgcctgaatc aaagccagtg ctcattccga
     7741 gatgcaggcc cgcagagtgc gtcccgaaca atttcatcgc tttacgcata aagcgatacg
     7801 ccaaatcaac cggcgataat ggcacgggtc cacctgaaag gaggcgccag cgatccgctg
     7861 atggtccagt gcggtcttca tgctgataat caaatttttg tgacttgtta gcatataaac
     7921 gacgaataaa gctttgcatt ttttcaaatg cctgcgccct gttctcttca cccaacgtgt
     7981 cgtgataaaa gcctggcagc agatgcagct ctttcagagg gctacgtaat ctctgataaa
     8041 aatcaatctg cggttggcga tgcaccacat agtcatcgcc tgatatcaga agttgcgtgg
     8101 ggagcgtaat cgccgccgca tctctaataa tacgttcaga cgttttatag agatcgagca
     8161 agatattgac ggcaatcgcc cgtgtgatca gcggatcatt attgaaactc gccacccgtt
     8221 gccgatcgtg ggtcaaatag cgtcttttca cataggaatt aataaaaaac agaccacgca
     8281 gacgatgcca tagcgtcagc gcaggacgtg ccagcggcac atacaattta accttaaagg
     8341 ccggagaagc cagcaccagc ccgcgaattg caggcgcata atcatgaacc catgtggcaa
     8401 ccagcactgc gccgacgctt tgcgcgatca caaccacctc ttccagtcca acctggctgt
     8461 cgctggcagc aaaacggaca aattcatcga catcctgcac tgaacgcgca agagatgggc
     8521 tataaccacg cggccccgaa gtttttccat gcccccgggc atcccatgca taaaaagcag
     8581 tatctggcat cgccagttca tcaacgatat gttgtagacg accagaatgt tcatggccac
     8641 gatgaaataa gacgatgacc tttttcgccc ctggctgtaa agcgggccag tgccgataaa
     8701 acaacgctgt attatcactg gtggtaaaaa aatgttcccc agagatgcgt gaattttcca
     8761 ttttctctat tttccttcag taacagcagt aacgaataaa ataaaaaaat accggagcgg
     8821 tgaaaattaa tgagtcaatc ctgtcaagga ttccgccatg tccaggtaat agtttcccgc
     8881 tatctttaac accaacatct cgtttgatgg ctgacatcac tacatcgccg caaaaaccac
     8941 taataccaat taacaaaccc gctaatattg cctgtaatgt atttagcggt gtcagtaacg
     9001 gtccaatgat catcgacgct atcatggtgg tgattacgcc gccgaccaga ccttccagcg
     9061 tttttcccgg gctgactttg gggaccactt ttcttctgcc gcaggatttc ccccataaat
     9121 actgtgcaat atcgtttgac gcggttaatg ccaaaagaaa taacaccagt aaggccccgc
     9181 cctggatatt catggatggc aacattaata actaggcggc atgactcaag gcgaaaacgg
     9241 tcattatcca gccccaaaaa atggcgctca cggtatgcag aaaaccggag gggtcaccca
     9301 caaaaactcg ccaggcggct aatatcagaa acccagccaa aggtataaat aacagaaaca
     9361 gctcaaagca attaaaaccg ataagcaaat agttaagagg aataacccaa tataaccacc
     9421 gcggaaaatg tacggatata agcatacaat attctttcaa tgccagaaaa cttatcagcg
     9481 caaaaaacgc caacgtcatc cagcgtggac ctgaaatcac cagtgaaaaa caaatgacga
     9541 tgccccacca ggtacgaata cgtaatgtaa cctcaccccc ttttctctcc ggtaaccgcc
     9601 atagcagaaa acggtttatc agcgttgcta aaattaacaa cgcg
//
LOCUS       DADMKF010000145         9579 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.148, whole genome shotgun sequence.
ACCESSION   DADMKF010000145 DADMKF010000000
VERSION     DADMKF010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9579
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.148"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(<1..158)
                     /locus_tag="JNC24_003288"
     CDS             complement(<1..158)
                     /locus_tag="JNC24_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 family protein"
                     /protein_id="HAY9159966.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPA"
     gene            complement(158..1084)
                     /gene="glsB"
                     /locus_tag="JNC24_003289"
     CDS             complement(158..1084)
                     /gene="glsB"
                     /locus_tag="JNC24_003289"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HAY9159967.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(1148..2536)
                     /gene="sad"
                     /locus_tag="JNC24_003290"
     CDS             complement(1148..2536)
                     /gene="sad"
                     /locus_tag="JNC24_003290"
                     /EC_number="1.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate-semialdehyde dehydrogenase"
                     /protein_id="HAY9159968.1"
                     /translation="MTITPATHAISINPATGEQLSVLPWAGANDIENALELAAAGFRD
                     WRETNIDYRAEKMRDIGKALRARSEEMAQMITREMGKPINQARAEVAKSANLCDWYAE
                     HGPAMLKAEPTLVENQQAVIEYRPLGTILAIMPWNFPLWQVMRGAVPIILAGNGYLLK
                     HAPNVMGCAQLIAQVFKDAGIPQGVYGWLNADNDGVSQMIKDSRIAAVTVTGSVRAGA
                     AIGAQAGAALKKCVLELGGSDPFIVLNDADLELAVKAAVAGRYQNTGQVCAAAKRFII
                     EEGIASAFTERFVAAAAALKMGDPRDEENTLGPMARFDLRDELHHQVEKTLAQGARLL
                     LGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGPVAAITVAKDAEHALELANDSEFG
                     LSATIFTTDETQARQMAARLECGGVFINGYCASDARVAFGGVKKSGFGRELSHFGLHE
                     FCNIQTVWKDRI"
     gene            2637..3518
                     /locus_tag="JNC24_003291"
     CDS             2637..3518
                     /locus_tag="JNC24_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY9159969.1"
                     /translation="MDLTQLEMFNAVAEAGSITQAAAKVHRVPSNLTTRLRQLETELG
                     VDLFIRENQRLRLSPAGHNFLRYSQQILALVDEARSVVAGDEPQGLFSLGSLESTAAV
                     RIPATLAEFNHRYPKIQFSLSTGPSGTMLEGVLEGKLNAAFIDGPINHTAIDGIPVYR
                     EELMIVTPQGHAPVIRASQVNGSNIYAFRTNCSYRRHFESWFHADGAAPGTIHEMESY
                     HGMLACVVAGAGIALIPRSMLESMPGHHQVEAWPLAEQWRWLTTWLVWRRGAKTRPLE
                     AFIQLLDVSDSAKQSYQ"
     gene            3595..4710
                     /gene="yneK"
                     /locus_tag="JNC24_003292"
     CDS             3595..4710
                     /gene="yneK"
                     /locus_tag="JNC24_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HAY9159970.1"
                     /translation="MVTPVSISNYISLPDDFPVRNIAPQVKEVLKDFIDALSTIICDE
                     EWRTSLNINSATKKIFNNLDNLSYIQRTSFRGNDTLYNEKVQFKLTYPVKNGRHKENI
                     EFQVVINLSPIYLDNFRHDGEINIFCAPNPKPVTMGRVFQTGVERVLFLFLNDFIEQF
                     PMFNPGVPIKIAHTPHIEPLPPDHHTAADYLRQFDLLVLNFISRGNFVILPRLWNNSE
                     VHRWFVNKDPNLITAILDITDSELKEDLLQSLMDSLGSNKHVLPEVCICFLFLLAEQE
                     SPHFQDLFLFFANMLLHYHQFMNPNESDLNDVLMPASLSDDKIIKHMARRTLELFVKN
                     ETPPKVTHEDLVKNRPRSPVRPPIPATSKTPDLPERH"
     gene            4860..6050
                     /gene="ydeA"
                     /locus_tag="JNC24_003293"
     CDS             4860..6050
                     /gene="ydeA"
                     /locus_tag="JNC24_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HAY9159971.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALITLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(6075..6740)
                     /gene="marC"
                     /locus_tag="JNC24_003294"
     CDS             complement(6075..6740)
                     /gene="marC"
                     /locus_tag="JNC24_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HAY9159972.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            6952..7386
                     /gene="marR"
                     /locus_tag="JNC24_003295"
     CDS             6952..7386
                     /gene="marR"
                     /locus_tag="JNC24_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HAY9159973.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            7406..7789
                     /gene="marA"
                     /locus_tag="JNC24_003296"
     CDS             7406..7789
                     /gene="marA"
                     /locus_tag="JNC24_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HAY9159974.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            7821..8039
                     /gene="marB"
                     /locus_tag="JNC24_003297"
     CDS             7821..8039
                     /gene="marB"
                     /locus_tag="JNC24_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HAY9159975.1"
                     /translation="MKPLSSAIAAALILFSAQGVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(8096..9535)
                     /locus_tag="JNC24_003298"
     CDS             complement(8096..9535)
                     /locus_tag="JNC24_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAY9159976.1"
                     /translation="MSGFKKGFLWGGAVAAHQLEGGWNEGGKGISIADVMTAGAHGVP
                     REVTEGVIDGLNYPNHEAIDFYHRYKTDIQLFAEMGFKCFRTSIAWTRIFPQGDEQEP
                     NEEGLQFYDDLFDECLKQGMEPVVTLSHFEMPYHLVTKYGGWRNRKLIDFFIRFASTV
                     FTRYKEKVKYWMTFNEINNQVNFSESLCPFTNSGILYSPEEDINEREQIMYQAVHYEL
                     VASALAVQTGKSINPEFNIGCMIAMCPIYPLTCAPNDMMMATKAMHRRYWFTDVHARG
                     YYPQHMLNYFARKGFNLDITPEDNAILASGCVDFIGFSYYMSFTTQFSPDNPQLDYVE
                     PRDLVSNPYIDTSEWGWQIDPAGLRYSLNWFWDHFQLPLFIVENGFGAVDQRQADGTV
                     NDHYRIDYFASHIREMKKAVVEDGVDLIGYTPWGCIDLVSAGTGEMKKRYGMIYVDKD
                     NEGKGTLERIRKASFYWYRDLIANNGENI"
BASE COUNT         2467 a         2461 c         2163 g         2488 t
ORIGIN      
        1 gccggagcaa gacgtttagc gacaaaatcc gccgcatgtt gttcgatgac tggcgcgcct
       61 ttctccaggc aatactgacg ctctttcatg cccagacgaa agcgagagcg aaattttgaa
      121 cgggataaac gcgcgaagag tggatcgaga gactgcatta ataaaccgaa cgccctaact
      181 gtttcgtcaa ttgttcaagg acggcgatac ccgcaagcga gttacctgca tcatccagtt
      241 ccggactcca gactgcgatg gccatttcat gcggaacaat cgccacaata ccgccaccaa
      301 cgccagattt cgccggtagt cccacccgcc aggcaaactc ccccgcgttc tggtacatac
      361 cactggtcgc catcagcgcg ttaatttgcc gcgcctgcat tggcgtcact acaggttcat
      421 caatatgaat agctttcccc tgattagcca gaaagacaaa cgtccgagcc agctctacac
      481 agctcatttt cagagcgcag taatgaaagt agttttgcag aacggttgtc acgtcatgat
      541 ggaaattgcc aaacgacttc atcagccagg cgatagccgc atttcgcgcg gaatgttcaa
      601 attcggaacg cgctaccacc gtatcgtatg aaatatcaga cacaccgctt aagccgcgca
      661 cgacttccag catacgttgc cgtggtgcgc ttaatcgccc ttgcaacata tcgcagacca
      721 ccagcgcacc agcattaata aacggattac gcggaatacc ctgttccatt tccagttgca
      781 ctaaagaatt gaacggtgat ccagacggat ctttgccgac gcgttgccag atttcctctt
      841 cggagtaatg acgcatggcg acaacgagac tcagcacttt ggaaatagac tgaatggaaa
      901 aacgttcttg cgcgtctccg gcctgaaaaa gctgtccgtc aacggtacag atagcaatgc
      961 ccaatcggga accgtctact gtagccagcg ccggaatata atccgcgact ttaccctgac
     1021 caatgagcgg ccgcacttgc cgcaagatgt tttctaaaat tgcattatcc atggcgactg
     1081 ccactttcta ctcctggacc gcaggtctga aaagacctgc gagtatatca gagctgaata
     1141 tgtcgcgtca gatccggtct ttccacaccg tctggatatt acagaattcg tgtaagccga
     1201 aatgggaaag ctcacgacca aagccactct ttttcacgcc accaaaggcc actcgcgcgt
     1261 cgctggcaca ataaccattg ataaacaccc caccgcattc cagacgtgcc gccatctgtc
     1321 tggcctgtgt ttcgtcggtg gtaaaaatgg tcgctgaaag gccgaactca ctatcattag
     1381 ccagttccag cgcatgttct gcatctttcg caacggtgat tgccgcaacg gggccaaaca
     1441 tttcttcccg aaacgcggtc atttctgggg taacattcgc cagaaccgtt ggcggatagt
     1501 agttacctgc cccagccatc ttttccccgc ccagtaacaa acgcgcaccc tgcgccaggg
     1561 ttttctctac ctgatgatgc agctcatcac gtaaatcaaa acgagccatt ggtccgagag
     1621 tgttctcttc gtcacgggga tcgcccattt tcaaggctgc cgcagctgcc acaaaacgtt
     1681 cggtaaatgc agaagcaatt ccctcttcga taataaagcg ttttgccgct gcacatacct
     1741 gtccggtatt ctgataacgt ccggctaccg ccgctttaac cgccagttcc agatcggcat
     1801 cgttaagcac aataaacgga tccgaaccgc ccagttccag tacgcatttt ttcagtgccg
     1861 ctccagcctg tgcgccaata gccgctccag cacgaacact tccggtcacc gtgacagcag
     1921 caatgcgcga gtctttaatc atctgactga caccgtcgtt gtcggcattc agccagccat
     1981 atacgccttg cgggattccc gcatctttaa acacctgggc aatgagctga gcacatccca
     2041 tcacattcgg cgcatgttta agtaagtagc cgttacctgc aagaatgatg ggaacagcgc
     2101 cacgcatcac ctgccataac ggaaaattcc acggcataat cgccagaatc gtccccaacg
     2161 gtcgatactc aataaccgcc tgctgatttt ccaccagtgt aggttccgcc ttcagcattg
     2221 ccggaccatg ttctgcatac cagtcacaca aattcgccga tttcgccact tcagcgcgcg
     2281 cctggttgat tggtttgccc atttcgcggg tgatcatttg cgccatttct tcgctacggg
     2341 cgcgcagagc cttaccgata tcacgcattt tttcagcacg ataatctata tttgtctcgc
     2401 gccagtcgcg aaagcctgct gccgccagct caagtgcgtt ttcgatatcg ttagcgccag
     2461 cccacggcag cacagaaagt tgttcacccg tggcaggatt tattgaaatt gcatgagttg
     2521 ccggagtaat ggtcatcggg gtatctcctt tatgagtcat ggtatgaaga tacgcagatt
     2581 tactcttgct ttaaaatgaa taatattaag ccacttattc acgaatcgag aatgttatgg
     2641 atctgaccca actggagatg ttcaacgcgg ttgccgaagc tggcagcatt acccaggctg
     2701 cagcaaaagt gcatcgcgta ccgtcgaatc tcactacccg tctacgccag ctggaaacag
     2761 aactgggggt agatctgttt attcgcgaga atcagcgttt acgtctctct cctgccgggc
     2821 ataacttttt acgctatagc caacaaattc tcgcgttagt ggatgaagcg cggagcgttg
     2881 tcgctggcga tgagccgcaa ggtttatttt ctcttggttc gctggaaagc accgcagcag
     2941 tgcgcattcc ggccacgctg gcggaattca accatcgtta tcccaaaatt cagttttcac
     3001 tttccaccgg cccttccggc actatgctgg aaggagtgct ggagggaaaa ctgaatgcgg
     3061 catttattga tgggcccatt aaccatactg ccattgacgg gataccggta taccgcgagg
     3121 agctgatgat cgtcacgcca caaggtcatg cgccagtaat ccgcgccagt caggttaacg
     3181 gcagtaacat ttatgccttc cgcaccaatt gttcgtatcg tcgccacttc gagagctggt
     3241 ttcatgctga cggtgccgct ccgggaacta ttcacgagat ggagtcgtat cacgggatgt
     3301 tggcctgtgt ggtcgcagga gcaggcattg cgcttattcc acgctctatg ctggaaagta
     3361 tgccggggca tcaccaggtt gaagcgtggc cgttagctga gcaatggcgt tggttaacaa
     3421 cctggctggt ctggcgtcgc ggtgcgaaaa cgcgtccgct tgaggcattt attcaactac
     3481 tggatgtgtc tgactcggca aaacagagtt accaatgaac tattttgata attcttgcgg
     3541 ctaatatgct ctatatagtg atgttccgat gacttatgac tatatggggc aaatatggtt
     3601 acgccagtaa gcatcagcaa ttacatatct cttcctgatg attttcccgt tcgtaatatt
     3661 gcaccacagg taaaagaagt tttaaaggac tttattgatg cgctaagtac aataatatgt
     3721 gatgaagagt ggcgcacgtc tttaaacatc aactcagcca caaaaaagat atttaataac
     3781 cttgataact tatcatatat tcagaggact tctttccggg gtaacgacac gctatacaat
     3841 gaaaaggttc agtttaaact cacttatccc gtcaagaatg gaagacacaa agaaaatatt
     3901 gaatttcagg tagtaataaa tttaagccct atttatttag ataattttcg ccatgatgga
     3961 gaaattaata ttttttgcgc tcccaaccca aagcctgtca ctatggggcg cgtatttcag
     4021 accggcgtcg agcgtgtgct ctttctgttt ctgaatgatt ttattgaaca atttccaatg
     4081 ttcaaccctg gcgttccgat caaaatagcg catacaccac atattgaacc cctgcctccg
     4141 gatcaccata ccgctgcaga ttacttacgc cagtttgatt tgcttgtcct gaattttatc
     4201 tctcgcggta attttgtcat actcccccga ttatggaata actctgaggt tcacagatgg
     4261 ttcgtcaata aagatcctaa cttgatcacc gccattctcg acataacgga cagcgaatta
     4321 aaagaggatt tattgcaaag cctgatggat tcattaggtt ctaacaaaca tgtactaccc
     4381 gaagtctgca tctgcttttt attcctttta gcagaacaag aatctcctca ttttcaggac
     4441 ttgtttttat ttttcgccaa tatgttattg cactatcacc aatttatgaa tcccaatgaa
     4501 agtgatttga atgacgtgtt gatgccagca tcattaagtg atgataaaat tatcaagcat
     4561 atggcgcgca ggaccctcga actgtttgta aaaaatgaaa caccgccaaa agttactcac
     4621 gaagacctgg tgaaaaacag gcctcgctcc cctgtcagac cacctatacc cgcaacctcc
     4681 aaaacgccag acctccctga acgtcattaa accgtgatgt taccgactct ctgacgcgga
     4741 acagaatcag cgtcagagaa acggaaaacg cgatccagat cacaaataca ttgtattcac
     4801 atcattaacc gttttaagat catttcatca ctttttcgca actcacccga taatctgtta
     4861 tgacaacaaa cactgtttcc cgcaaagtgg cgtggctacg ggtcgttacg ctggcagtcg
     4921 ccgccttcat cttcaacacc accgaatttg tccctgttgg cctgctctct gacattgcgc
     4981 aaagttttca catgcaaacc gctcaggtcg gcatcatgtt gaccatttac gcatgggtag
     5041 tagcgctaat gtcattgcct tttatgttaa tgaccagtca ggttgaacgg cgcaaattac
     5101 tgatctgcct gtttgtggtg tttattgcca gccacgtact gtcgttttta tcgtggagct
     5161 ttaccgttct ggtgatcagt cgcattggtg tggcttttgc acatgcgatt ttctggtcga
     5221 ttacggcgtc tctggcgatc cgtatggctc cggccgggaa gcgggcacag gccttgagtt
     5281 taattgccac cggtacagca ctggcgatgg tcttaggttt acctctcggg cgcattgtgg
     5341 ggcagtattt cggttggcga atgaccttct tcgcgattgg tattggggcg cttatcaccc
     5401 ttttgtgcct gattaagtta cttcccttac tgcccagtga gcattccggt tcattgaaaa
     5461 gcctcccgct attgttccgc cgcccggcat tgatgagcat ttatttgtta actgtggtgg
     5521 ttgtcaccgc ccattacacg gcatacagct atatcgagcc ttttgtgcaa aacattgcgg
     5581 gattcagcgc caactttgcc acggcattac tgttattact cggtggtgcg ggcattattg
     5641 gcagcgtgat tttcggtaaa ctgggtaatc agtatgcgtc tgcgttggtg agtacggcga
     5701 ttgcgctgtt gctggtgtgt ctggcactgc tgctacctgc ggcgaacagt gaaatacacc
     5761 tcggggtgct gagtattttc tgggggatcg cgatgatgat catcgggctt ggtatgcagg
     5821 ttaaagtgct ggcgctggca ccagatgcta ccgacgtcgc gatggcgcta ttctccggca
     5881 tatttaatat tggaatcggg gcgggtgcgt tggtaggtaa tcaggtgagt ttgcactggt
     5941 caatgtcgat gattggttat gtgggcgcgg tgcctgcttt tgccgcgtta atatggtcaa
     6001 tcattatatt tcgccgctgg ccagtgacac tcgaagaaca gacgcaatag ttgaaaggcc
     6061 cattcgggcc ttttttaatg gtacgtttta atgatttcca ggatgccgtt aataataaac
     6121 tgcacaccca tacataccag caggaatccc atcagacggg agatcgcttc aatgccaccc
     6181 ttgcccacca gccgcataat tgcgccggag ctgcgtaggc ttccccacaa aataaccgcc
     6241 accaggaaaa agatcagcgg cggcgcaacc atcagtaccc aatcagcgaa ggttgaactc
     6301 tgacgcactg tggacgccga gctaataatc atcgctatgg ttcccggacc agcggtactt
     6361 ggcattgcta gcggcacaaa ggcaatattg gcactgggtt catcttccag ctcttccgac
     6421 ttgcttttcg cctccggtga atcaatcgct ttctgttgcg gaaagagcat ccgaaaaccg
     6481 ataaacgcga cgattaagcc gcctgcaatt cgcagaccgg gaatcgaaat gccaaatgta
     6541 tccatcacca gttgcccggc gtaatacgcc accatcatga tggcaaatac gtacaccgag
     6601 gccatcaacg actgacgatt acgttcggca ctgttcatgt tgcctgccag gccaagaaat
     6661 agcgcgacag ttgttaatgg gttagctaac ggcagcaaca ccaccagccc caggccaatt
     6721 gctttaaaca aatctaacat tggtggttgt tatcctgtgt atctgggtta tcagcgaaaa
     6781 gtataagggg taaacaagga taaagtgtca ctctttagct aaccttgcat cgcattgacc
     6841 aaaacttgaa ccgatttagc aaaacgtggc atcggtcaat tcattcattt gacttatact
     6901 tgcctgggca atattatcct ctgcaactaa ttacttgcca gggcaactaa tgtgaaaagt
     6961 accagcgatc tgttcaatga aattattcca ttgggtcgct taatccatat ggttaatcag
     7021 aagaaagatc gtctgcttaa cgagtatctg tctccgctgg atattaccgc ggcacagttt
     7081 aaggtgctct gctctatccg ctgcgcggcg tgtattactc cggttgaact gaaaaaagtg
     7141 ttgtcggtcg acctgggagc actgacccgt atgctggatc gcctggtctg taaaggctgg
     7201 gttgaaaggt tgccgaaccc gaatgataag cgcggcgtac tggtaaaact taccaccagc
     7261 ggcgcggcaa tatgcgaaca atgccatcaa ttagttggcc aggacctgca tcaagaatta
     7321 acaaaaaacc tgacggcgga cgaagttgca acacttgagc atttgcttaa gaaagtcctg
     7381 ccgtaaacaa aaaagaggta tgacgatgtc cagacgcaat actgacgcta ttaccattca
     7441 tagcattttg gactggatcg aggacaacct ggaatcgcca ctgtcactgg agaaagtgtc
     7501 agagcgttcg ggttactcca aatggcacct gcaacggatg tttaaaaaag aaaccggtca
     7561 ttcattaggc caatacatcc gcagccgtaa gatgacggaa atcgcgcaaa agctgaagga
     7621 aagtaacgag ccgatactct atctggcaga acgatatggc ttcgagtcgc aacaaactct
     7681 gacccgaacc ttcaaaaatt actttgatgt tccgccgcat aaataccgga tgaccaatat
     7741 gcagggcgaa tcgcgctttt tacatccatt aaatcattac aatagctagt tgataacgtg
     7801 acaacgtcac tgaggcaatc atgaaaccac tttcatccgc aatagcagct gcgcttattc
     7861 tcttttccgc gcagggcgtt gcggaacaaa ccacgcagcc agttgttact tcttgtgcca
     7921 atgtcgtggt tgttccccca tcgcaggaac aaccaccgtt tgatttaaat cacatgggta
     7981 ctggcagtga taagtcggat gcgctcggcg tgccctatta taatcaacac gctatgtagt
     8041 tattgtcagg ctctgatcac agagcctgac atctccgtaa tatcttcctc aagtctcaaa
     8101 tattttcgcc attgttggcg atgagatccc gataccagta aaacgacgct ttacgtatcc
     8161 gttccagcgt tcccttccct tcgttgtctt tatcgacata aatcattccg tagcgctttt
     8221 tcatttcccc tgttccggca gaaaccaggt caatgcagcc ccacggggtg tagccaatta
     8281 agtcaacacc atcttcaact acggcttttt tcatttcccg aatatgggaa gcaaagtaat
     8341 caatgcgata gtgatcgttc accgtgccgt cagcttgtct ctggtcaacc gcaccaaatc
     8401 cattttcgac aataaacagc ggcaactgga aatgatccca gaaccagttg agtgaataac
     8461 gtagccctgc cggatcaatt tgccatcccc attcggatgt atcgatataa gggttgctga
     8521 ccaaatctcg tggttcaaca taatccagtt gcgggttatc gggagaaaat tgtgtcgtaa
     8581 aagacatgta gtagctaaaa ccgataaagt cgacacatcc actggcaaga atcgcgttat
     8641 cttctggtgt gatatcgagg ttgaatcctt tcctggcaaa gtaattcagc atatgttgcg
     8701 gataatatcc acgagcatga acatcagtaa accagtaacg acgatgcatc gctttcgtgg
     8761 ccatcatcat atcgttgggt gcacacgtca gaggatagat ggggcacatg gcgatcatac
     8821 agccaatgtt aaattcagga ttgatcgatt ttccagtctg taccgccagg gcactggcaa
     8881 ctaactcgta atgtaccgcc tggtacatta tttgttcgcg ctcattgata tcttcctctg
     8941 gcgaatacaa gataccggaa ttagtaaatg gacacaggct ttcgctgaaa ttcacctgat
     9001 tattgatttc gttaaacgtc atccagtact ttactttttc tttatagcgc gtgaagaccg
     9061 ttgatgcgaa gcggataaaa aagtcgatca gcttacggtt tcgccagcca ccatattttg
     9121 tcaccagatg ataaggcatc tcaaaatgcg aaagcgtcac cacaggttcc attccttgct
     9181 tcaggcattc atcgaacaga tcatcataaa attgtaaacc ctcttcattc ggctcctgtt
     9241 cgtcaccttg cggaaagatt cgtgtccagg caatggaagt tcgaaagcat ttgaatccca
     9301 tctcggcaaa taactgaata tctgttttat agcgatgata aaaatcaatt gcttcatgat
     9361 tgggataatt aagcccgtcg ataacgcctt ctgtcacttc acgcggcacc ccgtgagcgc
     9421 cagcagtcat cacatcagcg atactgatgc cttttcctcc ttcattccag ccaccttcca
     9481 actgatgcgc ggctaccgcg ccaccccata aaaaaccttt tttaaatcct gacatacaca
     9541 actccttaac taaaatagat taccaccaga cttccgcct
//
LOCUS       DADMKF010000146         9542 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.149, whole genome shotgun sequence.
ACCESSION   DADMKF010000146 DADMKF010000000
VERSION     DADMKF010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9542
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.149"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            <1..1321
                     /locus_tag="JNC24_003299"
     CDS             <1..1321
                     /locus_tag="JNC24_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAY9159977.1"
                     /translation="PQFRIPAELIQHDIDFVAAHGVKFEYGCSPDLTVEQLKNQGFHY
                     VLIATGTDKNSGVKLAGDNQNVWKSLPFLREYNKGTALKLGKHVVVVGAGNTAMDCAR
                     AALRVPGVEKATIVYRRSLQEMPAWREEYEEALHDGVEFRFLNNPERFDADGTLTLRV
                     MSLGEPDEKGRRRPVETNETVTLHVDSLITAIGEQQDTEALNAMGVPLDKNGWPDVDH
                     NGETRLTDVFMIGDVQRGPSSIVAAVGTARRATDAILSRENIRSHQNDKYWNNVNPAE
                     IYQRKGDISITLVNSDDRDAFVAQEAARCLECNYVCNKCVDVCPNRANVSIAVPGFQN
                     RFQTLHLDAYCNECGNCAQFCPWNGKPYEDKITVFSLAQDFDNSSTPGFLVEDCRVRV
                     RLNNQSWVLNIDSEGQFNNVPPELNDMCRIISHVHQHHHYLLGRVEV"
     gene            1324..2652
                     /gene="ssnA"
                     /locus_tag="JNC24_003300"
     CDS             1324..2652
                     /gene="ssnA"
                     /locus_tag="JNC24_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HAY9159978.1"
                     /translation="MLILKNVTAVQLHPAKVQEGVDIAIENDVIVAIGDALTQRYPDA
                     SYKEMHGRIVMPGIVCSHNHFYSELSRGIMANIAPCPDFISTLKNLWWRLDRALDEES
                     LYYSGLICSLEAIKSGCTSVIDHHASPAYIGGSLSTLRDAFLKVGLRAMTCFETTDRN
                     NGIKELQEGVEENIRFARLIDEAKKATSEPYLVEAHIGAHAPFTVPDAGLEMLREAVK
                     ATGRGLHIHAAEDLYDVSYSHHWYGKDLLARLAQFDLIDSKTLVAHGLYLSKDDIALL
                     NQRDAFLVHNARSNMNNHVGYNHHLSDIRNLALGTDGIGSDMFEEMKFAFFKHRDAGG
                     PLWPDSFAKALTNGNELMSRNFGAKFGLLEAGYKADLTICDYNSPTPLLADNIAGHIA
                     FGMGSGSVHSVMVNGVMVYEDRQFNFDCDSIYAQARKAAASMWRRMDALA"
     gene            2703..3482
                     /locus_tag="JNC24_003301"
     CDS             2703..3482
                     /locus_tag="JNC24_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     FAD-binding subunit"
                     /protein_id="HAY9159979.1"
                     /translation="MIEQFFRPDSVEQALELKRRYQDEAVWFAGGSKLNATPTRTDKK
                     IAISLQDLELDWVDWDNGALRIGAMSRLQPLRDARFIPAALREALGFVYSRHVRNQST
                     IGGEIAARQEESVLLPVLLALDAELVFGNGETLSIEDYLACPCDRLLTEIIIKDPYRT
                     CATRKISRSQAGLTVVTAAVAMTDHDGMRIALDGVASKALRLHDVEKQNLEGNALEQA
                     VANAIFPQEDLRGSVAYKRYITGVLVADLYADCQQAGEEAV"
     gene            3479..6418
                     /locus_tag="JNC24_003302"
     CDS             3479..6418
                     /locus_tag="JNC24_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000583599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     Mo/Fe-S-binding subunit"
                     /protein_id="HAY9159980.1"
                     /translation="MIIHFTLNGAPQELTVNPGENVQKLLFNMGMHSVRNSDDGFGFA
                     GSDAIIFNGNIVNASLLIAAQLEKADIRTAESLGKWNELSLVQQAMVDVGVVQSGYND
                     PAAALIITDLLDRIAAPTREEIDDALSGLFSRDAGWQQYYQVIELAVARKNNPQATID
                     IAPTFRDDLDVIGKHYPKTDAAKMVQAKPCYVEDCVTADACVIKMLRSPHAHALITHL
                     DVSKAEALPGVVHVITHLNCPDIYYTPGGQSAPEPSPLDRRMFGKKMRHVGDRVAAVV
                     AESEEIALEALKLIDVEYEVLKPVMSIDEAMAEDAPVVHDEPVVYVAGAPDTLEDDNS
                     HAAQRGEHMIINFPIGSRPRKNIAASIHGHIGDMDKGFADADVIIERTYNSTQAQQCP
                     TETHICFTRMDGDRLVIHASTQVPWHLRRQVARLVGMKQHKVHVIKERVGGGFGSKQD
                     ILLEEVCAWATCVTGRPVLFRYTREEEFIANTSRHVAKVTVKLGAKKDGRLTAVKMDF
                     RANTGPYGNHSLTVPCNGPALSLPLYPCDNVDFQVTTYYSNICPNGAYQGYGAPKGNF
                     AITMALAELAEQLQIDQLEIIERNRVHEGQELKILGAIGEGKAPTSVPSAASCALEEI
                     LRQGREMIQWSSPKPQNGDWHIGRGVAIIMQKSGIPDIDQANCMIKLESDGTFIVHSG
                     GADIGTGLDTVVTKLAAEVLHCPPQDVHVISGDTDHALFDKGAYASSGTCFSGNAARL
                     AAENLREKILFHGAQMLGEAVADVQLATPGVVRGKKGEVSFGDIAHKGETGIGFGSLV
                     GTGSYITPDFAFPYGANFAEVAVNTRTGEIRLDKFYALLDCGTPVNPELALGQIYGAT
                     LRAIGHSMSEEIIYDAEGHPLTRDLRSYGAPKIGDIPRDFRAVLVPSDDKVGPFGAKS
                     ISEIGVNGAAPAIATAIHDTCGIWAEESQSGHGNGYRCRNNFQHAANAERSLQSVAIT
                     RLSPLGI"
     gene            6351..6665
                     /locus_tag="JNC24_003303"
     CDS             6351..6665
                     /locus_tag="JNC24_003303"
                     /inference="COORDINATES: protein motif:HMM:NF019581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HAY9159981.1"
                     /translation="MLQTLNEAYKVLQLQGYRLSAYEAFYLATLGGAKSLGLDDLIGN
                     FLPGKEADFVVMEPTATPLQQLRYDNSVSLVDKLFVMMTLGDDRSIYRTYVDGRLVYE
                     RN"
     gene            6701..7084
                     /locus_tag="JNC24_003304"
                     /pseudo
     CDS             6701..7084
                     /locus_tag="JNC24_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311784.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(7120..7608)
                     /locus_tag="JNC24_003305"
     CDS             complement(7120..7608)
                     /locus_tag="JNC24_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HAY9159982.1"
                     /translation="MKSLIIVNPADCIGCRTCEVACVVAHPSEQELNADVFLPRLKVQ
                     RLDSISAPVMCHQCENAPCVGACPVGALTMGEQVVQTNSARCIGCQSCVSACPFGMIT
                     IQSLPGDTRQQIVKCDLCEQREEGPACVESCPTQALQLLTERELRRVRQQRIVASGEN
                     PL"
     gene            complement(7608..9527)
                     /gene="ygfT"
                     /locus_tag="JNC24_003306"
     CDS             complement(7608..9527)
                     /gene="ygfT"
                     /locus_tag="JNC24_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     YgfT"
                     /protein_id="HAY9159983.1"
                     /translation="MNKFIAAEAAECIGCHACEIACAVAHNQENWPLSHSDFRPRIHV
                     VGKGQAANPVACHHCNNAPCVTACPVNALTFQSDSVQLDEQKCIGCKRCAIACPFGVV
                     EMVDTIAQKCDLCNQRSSGTQACIEVCPTQALRLMDDKGLQWIKVARQRKTAAGKASS
                     DAQPSRSAALLPVNSRKGADKISASERKTHFGEIYCGLDPLQATYESDRCVYCAEKAN
                     CNWHCPLHNAIPDYIRLVQEGKIIEAAELCHQTSSLPEICGRVCPQDRLCEGACTLKD
                     HSGAVSIGNLERYITDTALAMGWRPDVSKVVPRIEKVAVIGAGPAGLGCADILARAGV
                     QVDVFDRHPEIGGMLTFGIPPFKLDKTVLSQRREIFTAMGIDFHLNCEIGRDITFSDL
                     TSEYDAVFIGVGTYGMMRADLPHEDAPGVIQALPFLTAHTRQLMGLPESEEYPLTDVE
                     GKRVVVLGGGDTTMDCLRTSIRLNAASVTCAYRRDEVSMPGSRKEVVNAREEGVEFQF
                     NVQPQYIACDEDGRLTAVGLIRTAMGEPGPDGRRRPRPVAGSEFELPADVLIMAFGFQ
                     AHAMPWLQGSGIKLDKWGLIQTGDVGYLPTQTHLKKVFAGGDAVHGADLVVTAMAAGR
                     QAARDMLTLFDTKAS"
BASE COUNT         2301 a         2559 c         2488 g         2194 t
ORIGIN      
        1 tcctcagttc cgtattcctg cagagttaat tcagcacgat atcgattttg ttgccgctca
       61 cggcgtgaaa tttgagtatg gctgctcacc cgatttaacc gttgaacagt taaaaaatca
      121 gggcttccac tatgttctga ttgccaccgg cactgataaa aatagcggtg tgaaactggc
      181 gggcgacaac caaaatgtct ggaaatcact ccccttcctg cgtgaataca acaagggtac
      241 agcgctcaag ctgggcaaac atgtggtcgt tgtcggggcg ggtaacaccg caatggactg
      301 cgctcgtgcg gcgttacgcg ttccaggcgt agaaaaagca acgatcgttt accgtcgttc
      361 actacaagag atgcccgcat ggcgcgaaga gtatgaagaa gcgttgcacg acggcgtaga
      421 gttccgtttc ctgaataatc cggaacgttt cgatgctgat ggcaccttaa ccttgcgcgt
      481 tatgtcgctt ggcgaaccgg atgagaaagg tcgtcgccgt ccggttgaaa ccaacgaaac
      541 agtaacactg catgtagaca gcctgatcac cgccattggt gaacagcagg atactgaagc
      601 cctgaatgcg atgggcgtgc cgctggacaa aaacggctgg ccagacgtcg accataatgg
      661 cgaaactcgt ctgactgacg tctttatgat cggcgacgta cagcgcggac catcctccat
      721 tgtcgctgct gtcggaaccg cgcgtcgggc gaccgatgcc atccttagtc gggaaaatat
      781 ccgttcccac cagaacgata aatactggaa caacgtcaat ccagcggaaa tctatcaacg
      841 taaaggcgat atctctatca cgctggtgaa cagtgacgat cgtgacgcgt ttgtcgccca
      901 ggaagccgct cgctgcctcg aatgtaacta cgtttgcaac aagtgtgtgg atgtctgccc
      961 gaaccgcgcc aacgtctcca ttgcagtccc tggcttccag aatagattcc agacgctgca
     1021 cctcgacgct tactgtaacg aatgcggcaa ctgcgctcag ttctgtccgt ggaacggtaa
     1081 accatacgaa gacaaaatca ccgtcttcag cctggcgcaa gactttgata acagcagcac
     1141 cccaggcttc cttgtggaag attgccgggt acgagtacgt ctgaataacc aaagctgggt
     1201 gttaaacatc gacagcgaag gtcagtttaa caacgtacca ccggagctga acgatatgtg
     1261 ccgcatcatc agccatgtcc accagcatca tcattatctg ctgggccgcg tggaggtgta
     1321 atcatgttga ttctgaagaa tgtcaccgca gtgcagttac acccggcgaa agtgcaggaa
     1381 ggcgttgata tcgctatcga aaatgacgtg attgtcgcta tcggcgatgc cctgacgcaa
     1441 cgctaccccg atgccagcta caaagagatg catggtcgga ttgtgatgcc gggaattgtc
     1501 tgctcgcaca accattttta ctcggagctt tcccgcggaa ttatggcaaa catcgcccct
     1561 tgcccggatt tcatctcaac gctgaaaaat ctctggtggc ggctcgatcg cgcccttgat
     1621 gaagagtcgc tctattacag cggactgatt tgttccctgg aagcgattaa gagcggatgt
     1681 acatcggtta tcgatcacca tgcctcgccg gcgtatatcg gcgggtcgct ctccacattg
     1741 cgcgacgcat ttttaaaagt tggcctgcgc gcgatgacct gttttgaaac tactgaccgt
     1801 aacaacggca tcaaagagtt gcaggaaggt gtagaagaaa acatccgttt cgcccgtttg
     1861 attgatgagg cgaagaaagc gacaagcgag ccgtatctgg tggaagcaca tatcggtgct
     1921 cacgcgccgt ttaccgtgcc ggatgccggt ctggagatgc tgcgtgaagc cgtgaaagcc
     1981 acaggccgtg gtttgcatat tcacgctgcg gaagaccttt acgacgtttc ctacagtcac
     2041 cactggtacg gcaaagacct gctggcacga ctggcgcaat tcgacctcat cgacagcaaa
     2101 acgctggtcg ctcatgggct gtacttgtcg aaagatgaca tcgccctact caatcagcgc
     2161 gatgcgttcc tggtgcataa cgcccgttca aacatgaaca accatgtcgg ctacaaccat
     2221 caccttagcg acatccgcaa cctggcattg ggaacggacg gcattggttc ggacatgttt
     2281 gaagagatga aatttgcctt ctttaaacat cgcgatgcgg gtggtccgct gtggcctgac
     2341 agttttgcca aagccctgac taacggtaac gaactgatga gccgcaactt tggcgcgaaa
     2401 tttgggcttc tggaagccgg ttacaaagct gatttgacca tttgcgatta caactcgccg
     2461 acgccgctgc tggcagacaa tatcgccggg catatcgctt tcggtatggg ctcaggcagc
     2521 gttcacagcg tgatggtcaa tggtgtgatg gtctatgaag accgtcagtt taacttcgat
     2581 tgcgattcca tttatgcaca agccagaaaa gccgctgcca gtatgtggcg tcggatggat
     2641 gcgctggcat aaatgacaga tgccctcttc ccgcagggaa gagggttaaa taaggaaaga
     2701 agatgattga acaatttttc aggcccgact ctgtcgaaca ggcgctggaa ctgaagcgcc
     2761 gctaccagga tgaagccgtc tggttcgccg ggggcagcaa actcaatgct acaccaaccc
     2821 gtaccgataa aaagattgcc atttccttac aggatctgga actggactgg gttgactggg
     2881 ataacggtgc actgcggatt ggtgcaatgt ctcgcttgca gccactgcgt gatgcgcgct
     2941 ttattcctgc agcactgcgt gaagccctcg gttttgttta ctcacgccat gttcgtaatc
     3001 agtcgaccat tggtggtgaa atcgccgccc ggcaggaaga gtcagtgctg cttcccgtcc
     3061 tgctagcact ggatgctgaa ctggtttttg gcaacggcga aacgctgtca atcgaggact
     3121 acctggcctg cccatgcgat cgcctgctaa ccgaaattat cattaaagat ccgtatcgca
     3181 cctgtgcgac ccgcaaaatt agccgttctc aggcaggttt aaccgtcgtg acggcagccg
     3241 ttgcaatgac agaccacgac ggtatgcgaa ttgcgctgga tggcgtcgcc agtaaagcac
     3301 tgcgtctgca cgatgtcgaa aaacaaaatc tggaaggcaa tgcacttgaa caggctgtcg
     3361 ccaacgccat tttcccgcag gaagatttgc ggggcagcgt ggcctataaa cgctatatca
     3421 cgggagttct ggtagccgac ctgtatgccg actgccaaca ggctggggag gaagccgtat
     3481 gatcatccac tttactttaa atggcgcgcc tcaggagcta accgttaatc caggcgaaaa
     3541 cgtgcaaaag ctgttgttta acatgggaat gcactctgta cgcaacagtg atgatggctt
     3601 cgggtttgcc ggttctgacg caataatctt taacggtaat atcgttaacg cgtccttgct
     3661 tattgccgca cagttagaga aggcagatat tcgtaccgca gaatctctgg gtaaatggaa
     3721 cgagttaagt ctggttcaac aggcaatggt tgatgttggc gtggtgcagt ctggttataa
     3781 cgatccagct gcagctctga ttatcaccga tcttctcgat cgcatcgccg cacctacccg
     3841 cgaagagatc gacgacgcgc tttctggttt gttcagccgc gatgcaggct ggcaacaata
     3901 ctatcaggtc attgaactgg cggttgcacg taaaaataat ccgcaggcca ccattgatat
     3961 cgctccgact ttccgtgacg accttgacgt cattggcaag cattatccta aaactgatgc
     4021 cgcgaaaatg gtgcaggcga aaccctgcta tgttgaagac tgcgtaacgg ctgacgcctg
     4081 cgtcattaaa atgttacgta gcccacacgc tcacgcactg attactcatc tggatgtcag
     4141 caaagctgaa gccttaccgg gcgtcgttca cgttattact cacctgaatt gccctgatat
     4201 ctactatacc ccggggggtc agagcgcacc ggaaccgtca ccgcttgacc gccgtatgtt
     4261 cggcaagaaa atgcgtcacg tcggcgatcg tgttgctgcg gtcgtcgcag aaagtgaaga
     4321 aattgcgctc gaagcactga agctcatcga cgttgaatat gaagtgctta agccggtaat
     4381 gtcgatcgac gaagcaatgg cggaagatgc gcctgtcgtg cacgatgaac cggtggtgta
     4441 tgttgctggt gcgccagata ctctggaaga cgataacagc catgcagccc agcgcggcga
     4501 gcatatgatc atcaacttcc cgatcggttc tcgccctcgc aaaaatatcg ccgccagtat
     4561 tcatggtcat attggcgata tggacaaagg ctttgccgat gccgatgtga tcattgagcg
     4621 aacctataac tcaacgcagg cacagcagtg cccgactgaa acacatatct gctttacccg
     4681 gatggacggc gatcgtctgg ttatccacgc ctccacccag gtaccatggc acttacgccg
     4741 ccaggtcgcg cgcctcgtgg gcatgaaaca gcataaagtt catgtcatta aagagcgagt
     4801 tggcggcggt tttggttcca aacaggacat cctgctggaa gaagtgtgcg cctgggcaac
     4861 ctgcgtgacc gggcgtccgg tactattccg ctacacccgt gaagaagagt ttattgctaa
     4921 cacctctcgt cacgtcgcga aagtcaccgt caaactggga gcgaaaaaag atggtcgcct
     4981 gacggcagtg aagatggatt tccgcgccaa cactggccct tacggcaacc actcactcac
     5041 cgtaccgtgt aacggaccgg cgctgtcgct gccgttatat ccgtgcgata acgtcgattt
     5101 ccaggtcacc acctactaca gcaacatttg cccaaatggt gcttatcagg gttatggcgc
     5161 accgaaaggt aacttcgcta tcaccatggc attagcggaa ctggctgaac agttacagat
     5221 cgaccaactg gaaattatcg aacgtaaccg ggtacacgaa gggcaagagc tgaaaattct
     5281 cggtgcaatc ggtgaaggta aagcgccgac ctccgttcct tccgccgcca gctgtgcact
     5341 ggaagagatc ctgcgtcagg gtcgcgagat gatccaatgg tcgtcaccga aaccgcaaaa
     5401 tggtgactgg cacatcggtc gcggcgtcgc cattatcatg cagaaatcgg gtatcccgga
     5461 tatcgatcag gctaactgca tgatcaaact ggaatcggac ggcaccttta tcgttcattc
     5521 tggcggtgcg gatattggta ctggtctgga tactgtagtg acgaaactgg cagcagaagt
     5581 gctgcactgc ccaccgcagg acgtgcatgt tatctccggt gataccgatc atgcgttgtt
     5641 tgataaaggc gcatatgcct cgtccggtac ttgcttctcg ggtaacgcgg cgcgtttggc
     5701 agcggaaaat ctacgggaga aaattctgtt ccacggcgcg caaatgttgg gtgaggcagt
     5761 ggcagatgtt caactggcaa cgccgggcgt cgtgcgcggc aagaaaggcg aagttagttt
     5821 cggggatatt gcccataaag gcgaaaccgg catcggcttt ggttcactgg tgggaactgg
     5881 cagttatatc acgcctgatt tcgccttccc gtatggcgca aacttcgctg aagttgccgt
     5941 caacacgcgt acgggtgaaa tccgcctgga taaattctac gccttgctgg actgcggtac
     6001 accggtcaac ccggaattag cattgggaca aatctacggt gctaccctgc gagctatcgg
     6061 ccacagtatg agcgaagaga tcatttatga cgccgaaggt cacccgttaa cgcgtgattt
     6121 acgcagttac ggcgcaccga aaattggtga cattccgcgt gatttccgcg ctgtgctggt
     6181 gccgagcgac gataaagtcg gcccgttcgg ggcgaaatcg atctcggaaa tcggtgtaaa
     6241 tggcgcagct ccggcgattg ctaccgcaat tcacgataca tgcggcatct gggcagaaga
     6301 aagtcaaagt gggcatggga acggatatcg gtgccggaac aactttcaac atgctgcaaa
     6361 cgctgaacga agcctacaaa gtgttgcaat tacaaggcta tcgcctctcg gcatatgaag
     6421 cgttttacct ggccacgctc ggcggagcga aatctctggg ccttgacgat ttgattggca
     6481 actttttacc tggcaaagag gctgatttcg tggtgatgga acccaccgcc actccgctac
     6541 agcagctgcg ctatgacaac tctgtttctt tagtcgacaa attgttcgtg atgatgacgt
     6601 tgggcgatga ccgttcgatc taccgcacct acgttgatgg tcgtctggtg tacgaacgca
     6661 actaataata aaactttaac atcctcgtga ggacatcatt atgtctggag acatcctaca
     6721 aacaccggac gcaccaaagc cacagggcgc gctggataat tattttaaaa ttaccgctcg
     6781 tggcagtacc gttcgtcagg aagtactggc tggcttaacg acctttctgg ccatggttta
     6841 ttccgttatc gtcgttccgg gaatgctggg caaagcagtt tttcctcccg cagctgtgtt
     6901 tgttgccacc tgtctggtcg cgggcttcgg ctcgttgctg atggggttgt aggccaattt
     6961 gccaatggcg attggttgtg cgatttcctt gacggcattt accgcattca gtctggtact
     7021 cgggcaacaa attagcgttc ctgtcgcact ggtcgcattt tacgcgggtg gttgggcaat
     7081 ctaatagatt ctccgcgcct ttcagcgcgg agatttcttt cacagaggat tctcaccgct
     7141 ggcaacaata cgctgctggc ggactcgcct gagttctctt tcagtaagca actgcagcgc
     7201 ctgtgtcggg caagattcaa cacaggctgg cccctcttcc cgctgttcac agagatcgca
     7261 tttcacaatt tgttgtcggg tatcccccgg caacgactga atggtgatca ttccaaacgg
     7321 gcacgcactg acgcaactct gacagccaat acaacgggca gaattggtct gcaccacctg
     7381 ctcgcccatc gtcagcgccc ccacggggca agcgccaaca caaggggcgt tttcacactg
     7441 atggcacatc accggcgcac tgatgctgtc cagtcgctgt accttcagcc ggggtaaaaa
     7501 gacatcggca ttcaactcct gttctgaagg gtgagcgacc acacaggcta cttcacaagt
     7561 acggcagcca atacagtcag ccggattaac gataattaac gatttcatca cgatgccttc
     7621 gtatcaaaca gagttaacat atcgcgcgcc gcctgccttc ctgcggccat tgcagtgaca
     7681 accagatccg cgccatgaac tgcatcacca ccagcaaaga cttttttcag atgcgtctgg
     7741 gtaggtaaat acccgacgtc accggtttga atcaggcccc atttatcgag tttaattccg
     7801 ctgccctgca accacggcat ggcatgcgcc tggaaaccaa aggccataat cagaacatcg
     7861 gcgggcaatt caaactctga acccgctacc ggacgaggac gacggcgacc atccggcccc
     7921 ggctcaccca tggcggtacg aatcaggccc accgcagtta aacgcccatc ttcgtcacaa
     7981 gcgatatatt gcggttgaac attgaactga aactcgacac cttcctcgcg cgcattaacc
     8041 acctctttgc gcgagcccgg catactgact tcatcacgac gatacgcgca ggtcacgctg
     8101 gcagcattga ggcgaatgga agtccgcaaa caatccattg tcgtatcgcc accgcccaat
     8161 accacgactc gcttaccttc cacgtccgtc agcggatact cttcagactc cggcaatccc
     8221 atgagctggc gggtatgggc agtcaggaac ggtagagcct gaatgacacc gggcgcatct
     8281 tcatgcggca gatctgctcg catcatcccg taagtcccca cgccgatgaa aactgcatca
     8341 tattcagaag ttaaatcgct aaaggtaata tcgcggccaa tttcacagtt aagatgaaag
     8401 tcgattccca ttgcggtgaa tatctctcgc cgctggctta ataccgtttt atcgagtttg
     8461 aaaggaggaa tgccaaaagt cagcataccg ccaatttctg gatggcgatc aaagacatca
     8521 acctgaactc ccgcgcgcgc cagaatatca gcacacccta accctgctgg cccagcgcca
     8581 atcaccgcca ctttttcgat acggggaaca actttgctga catcgggacg ccagcccatc
     8641 gccagcgcgg tatcggtgat gtagcgttcc agattaccga tagagactgc gccagagtga
     8701 tctttcaaag tacatgcgcc ttcacaaaga cggtcctgtg ggcataccct gccgcagatt
     8761 tctggtaagg aactggtctg gtggcaaagt tctgccgctt caataatctt tccttcctgt
     8821 accagacgga tgtaatccgg aatagcgtta tgcagcgggc aatgccagtt gcagttagct
     8881 ttttcggcac aataaacaca gcggtcactc tcataagtcg cttgcagtgg atccagcccg
     8941 caatagattt cgccaaagtg ggttttccgt tcactcgctg aaattttatc tgcgccttta
     9001 cgcgagttaa cggggagcaa cgctgcactg cgagatggct gagcgtctga tgacgctttt
     9061 cctgctgccg ttttgcgctg gcgagccacc tttatccact gtaacccttt atcgtccatc
     9121 agtcgtaacg cctgtgttgg gcagacttca atacaggctt gcgtgccgga actgcgctgg
     9181 ttacaaaggt cgcatttctg cgcaatcgta tcgaccatct caacgacgcc aaaggggcaa
     9241 gcgattgcgc atcttttaca gccaatacat ttttgctcgt ccagttgtac gctatcggac
     9301 tggaaagtca gagcattaac cggacaagcc gtaacacaag gggcattgtt gcagtgatgg
     9361 caggccaccg gattcgcagc ctggcctttc cctacaacgt ggatacgcgg tcgaaagtca
     9421 ctgtgactca gcggccagtt ttcttgattg tgtgccaccg cacaggcaat ttcacaagca
     9481 tgacagccta tacattccgc agcttcagca gcgataaact tattcatttg catccctttc
     9541 at
//
LOCUS       DADMKF010000147         9501 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.150, whole genome shotgun sequence.
ACCESSION   DADMKF010000147 DADMKF010000000
VERSION     DADMKF010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9501
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.150"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(149..1231)
                     /locus_tag="JNC24_003307"
     CDS             complement(149..1231)
                     /locus_tag="JNC24_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001053102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HAY9159984.1"
                     /translation="MNLPVVPPLNVIRERLQLIFPEGTENRGYLIREIAAKTIFVMFY
                     AGAVEGLGRWIRPSQVVSMGDSQAALTDDASRESWVKMTFSKQKIRPADAWYAENTRE
                     PIRDETIKNGLIPCNAIVERKGIPTTSSRPRYSLNRSFAALFDAGLEGDSLLSAISSW
                     QENHLNKAALARLRLLKSGAVIAADAVVVTFPNGEKRTLAPGPSSTIAKAVIEVFAPN
                     FLKNPTVLWLSESGNKVVAQDDVLAKALGLNIDASKALPDIILIDLGEDRTGSDMLVV
                     FTEVVATDGPINRERKVTLTHLAIDAGFSEKNLAFLTAFMDRSALPFKKAIPELAWGS
                     YAWFASEPEHLIDLRDGYPVKISQRK"
     gene            complement(1228..3060)
                     /locus_tag="JNC24_003308"
     CDS             complement(1228..3060)
                     /locus_tag="JNC24_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HAY9159985.1"
                     /translation="MARMFDTLGFYIMNVQHFDFFTDNEFSNLERYQTCKAMAKGYAG
                     NYKTGQSRLMHARSYCFSIVAAYWDTLAKKQKSSIKMRAVPHTVYIEDIPCDARELAE
                     RTGELIAQFPAEDAGYLIGSIYTVMLPSAYRSELGAYYTPPPLVARLLDLAEKSGVDF
                     SHASVIDPACGGGAFLAPVAIRMLKKDKGSSPEWMLRRISRRLKGIEIDPFAAWMSLV
                     LLESVLMPLCVKVKRRLPEDTIIVADALQQDKFSGYDLVVGNPPYGRVTLDIKTREKY
                     SRSLFGHANLYGLFTDLAVRMVKEKTGVIAFLTPTSFLGGQYFTALRTLLTEKTTPYA
                     LDFVTDRDGVFDDVLQETMLTAFKAGEHKVRAQVSSLVPKGLNKAKIEKIGNVDIEKG
                     GATWLLPRVKDDACFLNVLKQMSTRLADLGYSVSTGQLVWNRFKSQLRTTKGKNSYPL
                     VWAESITSAGFRFSADRKNHVPYIDITPQQGFLVTKSECVLVQRTTSKEQDRRILAAI
                     LPQGFIDETGGVVVENHINIVYSNGLFSAVRPDVIDMLLNSHVVDRAFRCISGSVAVS
                     AYELNSIPLPSLEQVMEIQSLIDAGVHRRIIERTIAGFYGVMIA"
     gene            complement(3275..3688)
                     /locus_tag="JNC24_003309"
     CDS             complement(3275..3688)
                     /locus_tag="JNC24_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9159986.1"
                     /translation="MKLAIRLQLPLAFASKERSMEIKPEDELSNIVLFPVKEDNPRNQ
                     VNFLYEPSERPYCHHASVRVDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVRL
                     LRQEERERRKNIEKLIQIERNAKARIRRVTKYSPE"
     gene            complement(3689..4111)
                     /locus_tag="JNC24_003310"
     CDS             complement(3689..4111)
                     /locus_tag="JNC24_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HAY9159987.1"
                     /translation="MAKVFTQEEREKIKGQVVELVRQSGRETLRQLEAKTGATRYLIS
                     VLARELVASGDVYNSGYGLFPSEQARKDWQNARKKLSRAKLKKPSVVDPDLIWSLPDG
                     EIRRYDRHQNIICRECRKSEVMQRVPAFYQGDFQEAVL"
     gene            complement(4127..4852)
                     /locus_tag="JNC24_003311"
     CDS             complement(4127..4852)
                     /locus_tag="JNC24_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HAY9159988.1"
                     /translation="METVFDALKALKRASSHVVASRLGISREDAVNELWKLKRRGEAD
                     NKGSMWWLIQAGESEPVSPVPKVTAQMLTEAIEQHGPQTADELALMFGITSRRANSSL
                     AMAISKGRLIRVNQGGKFRYCIPGADLPAEPKAASIAETDGKVFPQPAGVALPVQEAA
                     TQEEIKTETVADIVQSLPSFTETQADDLVLPSLHMANRELRRAKSHVQKWERVCAALR
                     ELNKHRDIVRQIVDSSGRIVSEK"
     gene            complement(4874..5620)
                     /locus_tag="JNC24_003312"
     CDS             complement(4874..5620)
                     /locus_tag="JNC24_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAY9159989.1"
                     /translation="MKNIAAAGVLERIHRLAPQGAVPPYRTVEEWREWQLAEGRKRSE
                     EINRQNRQLRVEKILNRSGIQPLHSKCSFANYQVQNDGQKYALSQAKSIADELMTGCT
                     NFVFSGKTGTGKNHLAAAMGNRLMAKGRSVIIVTESDVMSVLHDSYDNGKSGEKFLQE
                     LCGVDLLVLDEIGIQRETKNEQVVLHQIIDRRTASLCSVGMLTNLNHAAMSTLLGERI
                     MDRMTMNGCRWVTFNWDSWRSNVSFPGVVK"
     gene            complement(5627..6691)
                     /locus_tag="JNC24_003313"
     CDS             complement(5627..6691)
                     /locus_tag="JNC24_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer"
                     /protein_id="HAY9159990.1"
                     /translation="MANAWLRLWHDMPNDPKWRTIARVSGQPIATVMAVYIHLLVSAS
                     RNVTRGHIDVTTEDLASALDVTEEVIDSILQTMQGRVLDGDLITGWEKRQVLKEDNGN
                     ISQTAKSPAERKRAQRERERKREQNGDCHGASRNVTHMSRRVTTDTDKDTDKEDQNTM
                     VHGVKNATNQAGDVQTVNPGQPAGSTPEADSAYALKADSGAVQQVMTARPEQSLQLQQ
                     PEADSAIQREADRVAPENTGQPVGRVDYPDVFEQVWREYPLRAGANPKKSAFSAWKAR
                     LREGVPPETMLDGVRRYARYLAATGKAGTEFVQRATTFFGPDRNFENPWLLPVSGTNN
                     QRCVNHISEPDTEIPPGFRG"
     gene            complement(6763..7188)
                     /locus_tag="JNC24_003314"
     CDS             complement(6763..7188)
                     /locus_tag="JNC24_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HAY9159991.1"
                     /translation="MKIKHEHIRMAMKAWAHPDGEKVPAAEITRVYFELGMTFPVLHN
                     DSKHNTLYLNTQKIFRWLDKDTPDAVEKIQALLPAIERAMPPPLVARMRSHSSAYFRE
                     LVETHERLVRDADDFVAVAIAGFNQMNRGGPAGNAVAVH"
     gene            complement(7185..7448)
                     /locus_tag="JNC24_003315"
     CDS             complement(7185..7448)
                     /locus_tag="JNC24_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY9159992.1"
                     /translation="MNLGEYLHHFHITQSSFAEIVGASQGMVSHVITGRAKLTGEKIL
                     RWCEATGWVVTPHEIDSKTYPNPTDGLPAEFQANTQPSAGVDS"
     gene            7560..8060
                     /locus_tag="JNC24_003316"
     CDS             7560..8060
                     /locus_tag="JNC24_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001343764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAY9159993.1"
                     /translation="MKRKSLSDTDLNAARRLKEIWMAKKSQLGLTQERAAEIMGFSTQ
                     GAVSHYLNGQTPLNLEAVLKFAGLLKVPPESIRPDMVDLLQIARMYCREPQQDNIVAL
                     PADTEQTEDELPFAVEPMERDLIHTFRAFPKEDQEHMLQEMKDKKESMDRTVARWLAA
                     QKGRRA"
     gene            8080..8274
                     /locus_tag="JNC24_003317"
     CDS             8080..8274
                     /locus_tag="JNC24_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAY9159994.1"
                     /translation="MSTVLSPMVSEFETVEQENSYNEWLRAKVAASLADPRPAIPHDE
                     VMAEMEQIIAQIAAATHRGE"
     gene            8274..8564
                     /locus_tag="JNC24_003318"
     CDS             8274..8564
                     /locus_tag="JNC24_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012601411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAY9159995.1"
                     /translation="MLPVLWLPSARDDLRQIVAYIAKENPAAARRMKIRIETSVLPLT
                     EHPYLYPPSERVLGLREIVTHPNYIILYRVTASSIEVVNIVHSRRQYPGKNS"
     gene            8844..8996
                     /locus_tag="JNC24_003319"
     CDS             8844..8996
                     /locus_tag="JNC24_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 domain-containing protein"
                     /protein_id="HAY9159996.1"
                     /translation="MDTIDLGNNESLVCGVFPNQDGTFTAMTYTKSKTFKTESGARCW
                     LARNTD"
     gene            9008..9373
                     /locus_tag="JNC24_003320"
     CDS             9008..9373
                     /locus_tag="JNC24_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001320327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9159997.1"
                     /translation="MEFKDLPSDVQKTAAHTLHSVLREIGKDIASEPAKDLARKIKTA
                     FVELYNVGTDSETVETKTVSSPIFSLEPSVLSGEICTEISSELLPVIREAIRRRGLDG
                     SYDHDVLQVLRTMVTSLGI"
BASE COUNT         2444 a         2493 c         2016 g         2548 t
ORIGIN      
        1 cacagaacaa ttattgcttt tgccgttgat gacaataaag tttttatcgc aggcgtatac
       61 cacggtgggc aaatctatga aaccgatttt ttatccagtg caccgtaaaa cccccatcct
      121 tcagggcgtg gcggatatca aactttggtc attttctctg gctgatcttt accggataac
      181 catcccgaag atcaattaag tgctccggtt cagaagcaaa ccatgcataa gatccccacg
      241 ccaattctgg tattgccttt ttaaaaggca gagcacttct gtccataaat gccgtaagga
      301 atgcaagatt tttctcgctg aacccagcgt caatagccaa atgagtgaga gtaacttttc
      361 gttccctgtt tataggccca tctgtcgcaa ctacttcagt gaaaactaca agcatgtccg
      421 aacccgttcg gtcctctccc aaatcaatca aaataatatc tggaagtgct tttgaggcat
      481 caatattcag ccccaatgct tttgccaata catcatcctg agcaacaact ttattcccgg
      541 attcagacaa ccagagcaca gttggattct taagaaaatt aggagcaaaa acttcgatga
      601 cggccttagc tattgtactt gacgggcccg gagctaaagt tctcttctct ccatttggga
      661 aagtgacgac tacggcatca gcagcaatca ctgcccctga tttcaataaa cgcaacctag
      721 caagagcggc tttgttcaaa tgattttctt gccaggaact tattgcactc agcagcgaat
      781 ctccttcaag cccggcatca aagagtgctg caaaagagcg attcaaactg taacgaggac
      841 gagaggatgt ggtgggtata cctttacgct caacaattgc attgcaaggt attaggccat
      901 tttttatagt ttcatcacgt attggctcac gcgtattttc cgcataccat gcatcagcag
      961 gccttatctt ttgtttagag aaagtcatct ttacccagct ttctctggac gcatcatcag
     1021 taagagcagc ctgcgaatct cccatactta caacctggct gggccttatc catcgcccca
     1081 gcccctcaac cgcaccagca taaaacatga caaaaattgt ttttgcagct atttcccgaa
     1141 ttaaatatcc acgattctca gtcccttcag ggaaaattaa ttgtaatctt tcccggataa
     1201 cattcaatgg gggaacaaca ggcagattca tgcaatcatc actccataga aaccagcgat
     1261 agttctctca ataatccgtc tgtggacgcc agcatcaatt aacgattgaa tctccatcac
     1321 ctgctccaga gaaggcaacg ggattgaatt caattcatag gccgaaacag caacacttcc
     1381 gcttatacac ctgaacgccc gatcgacaac atgcgaatta agtagcatat caataacatc
     1441 agggcgaacc gcagagaaca atccgtttga atatacaata ttaatatggt tttcaactac
     1501 tacgccacca gtttcatcta taaatccctg aggtaaaatt gccgctaaaa tccttctgtc
     1561 ctgttccttc gatgttgtcc gctgaacaag cacacattca gattttgtca caaggaaacc
     1621 ctgttgtggg gttatatcga tatagggaac atgatttttt ctgtctgcac taaaacgaaa
     1681 accagctgaa gttattgatt ccgcccagac taatgggtag ctattttttc ctttcgtcgt
     1741 acgcaactgc gacttaaatc gattccagac taactgacct gtagagactg agtatcccag
     1801 atcagctaaa cgcgtagaca tctgttttaa tacattaaga aaacaggcat catctttaac
     1861 gcgtggcaaa agccacgttg ctccaccttt ttctatatca acattaccta ttttctcaat
     1921 ttttgctttg ttcaacccct tagggacaag tgaggatacc tgggcccgaa ctttatgttc
     1981 tcccgccttg aatgctgtca acattgtttc ctggagaaca tcgtcaaaga ccccatccct
     2041 gtcagtgacg aaatcaaggg catacggagt agttttttcg gtcaaaaggg ttcgcagcgc
     2101 ggtgaaatac tgcccaccaa gaaaagaagt tggcgtaaga aatgcaataa ctcctgtttt
     2161 ctctttaacc atgcgaacag ctaagtcggt gaacaatcca tatagatttg catgaccaaa
     2221 taacgaacga gaatattttt ctctcgtttt aatatcgaga gtgacacgtc cgtaaggcgg
     2281 attccctacc acaagatcat aaccagaaaa tttatcttgc tgaagcgcat cagcaacaat
     2341 aattgtatct tcaggcaatc tacgttttac ctttacacat agtggcatta gtacagattc
     2401 gagcaatacc aaactcatcc aagccgcaaa tggatcgatc tctatcccct ttaaacgacg
     2461 actgattctc ctcaacatcc attcagggga agaccccttg tcttttttta acattcgaat
     2521 ggctactggt gccaaaaatg caccgccacc acacgcagga tcaataaccg atgcatgaga
     2581 aaaatcaaca ccagactttt cagccaggtc caagagacgc gctaccaaag gtgggggggt
     2641 atagtaagcc cccaattccg aacggtaagc ggatggcagc attaccgtat aaattgaccc
     2701 aatcagatat ccagcgtcct ccgccggaaa ttgagcaata agctcacctg ttcgttcagc
     2761 gagctcgcgt gcatcacatg gaatgtcttc tatgtaaaca gtgtgcggta ctgccctcat
     2821 tttaatgctg cttttttgct tttttgcaag ggtatcccaa tatgcagcta cgatgctgaa
     2881 gcagtatgag cgggcatgca tcaaccttga ctgcccggtt ttgtaattgc ccgcataacc
     2941 tttggccatg gctttacaag tttgatatct ttccagatta gaaaactcat tatctgtaaa
     3001 aaaatcaaaa tgttgcacat tcataatgta aaatcctaat gtatcaaaca tgcgggccat
     3061 aacatctcat ggtggcgcat agcaaactgg cccgaagaat atcacgcacc aagtcgatct
     3121 cataacccgt tatgacaata aatctttgta gctgaactca cttggtgatt tgtcaatcat
     3181 tcctggcctc cagttcgtcc tgaatctctt tatcgagttc accgtatttg ttagtcctca
     3241 tcactcacga cttgacttcc agaaataacc ttgattattc ggggctatat tttgtcaccc
     3301 tgcgtatccg cgctttcgca ttacgctcaa tctggattag cttttctata ttttttcgcc
     3361 tttcccgctc ctcctggcgc aagagcctta catcatctgc cagtctggtt tctcttttcg
     3421 ccacagagag catccagtca aatggctcca caactgcacc gcaaatttta cagcggacct
     3481 gacgctcttt ttcgtcaacc cggacagaag cgtgatggca atatggtctt tccgatggct
     3541 cataaagaaa attaacctga ttacgagggt tatcctcttt taccggaaat aaaacaatat
     3601 tgcttaactc atcttctggt tttatttcca tgctcctctc ctttgatgcg aatgccagcg
     3661 gtaattgaag cctgatagct aatttcactc acagtaccgc ctcctgaaaa tcaccctgat
     3721 agaaagccgg tacacgctgc ataacttcac tcttccggca ctcgcgacag attatgttct
     3781 gatgtctgtc gtagcggcgt atttctccgt ccggtaatga ccagataagg tccggatcaa
     3841 ccacagatgg tttcttcagc tttgccctcg agagtttttt gcgggcgttt tgccagtcct
     3901 tacgagcctg ttcagacggg aataacccgt agccagagtt gtatacatcc ccactggcaa
     3961 ccagctctct ggcgagaacg cttattaaat atcttgtcgc cccggtttta gcttccagtt
     4021 gtcgtaacgt ctcgcgccca ctctggcgta cgagttcaac aacctgcccc ttaatttttt
     4081 cccgctcttc ctgtgtaaat acttttgcca taagcgcccc cggcaatcac ttttccgaca
     4141 caatacgacc ggaggaatcg acaatctgtc gaacaatatc ccggtgcttg ttcagctccc
     4201 gcaacgcggc gcagacacgc tcccacttct ggacatgact tttcgcccga cgcagttcgc
     4261 ggtttgccat atgcagcgat ggtaaaacca ggtcatccgc ttgcgtttcg gtgaacgatg
     4321 gcaacgactg cacaatgtcc gccacagttt ctgttttaat ttcttcctgt gttgcagctt
     4381 cctgtaccgg taacgcaaca cctgctggct gaggaaagac tttaccatcc gtttccgcta
     4441 tggatgcggc tttcggctct gccggtaaat cagcgcccgg tatgcagtac cgaaatttac
     4501 cgccctgatt cacgcgaatc agacgccctt tgctgattgc catggccagc gatgaattcg
     4561 cccggcggga ggtaatcccg aacatcagtg ccagctcatc cgccgtttgt gggccatgtt
     4621 gttcaatcgc ctcagtcagc atttgcgctg tcactttcgg taccggtgac actggttcac
     4681 tttcaccagc ctgaatcagc caccacatcg aacccttgtt atccgcttca ccacggcgct
     4741 tcagtttcca cagttcgttg accgcatctt cacggctgat tccaaggcgg gacgccacca
     4801 catgtgaaga ggctcttttc agtgctttca gtgcgtcaaa tacagtttcc attaaaacat
     4861 cctccaacaa aaattacttc acaactcccg gaaagctgac atttgaacgc cagctatccc
     4921 agttaaacgt cacccaccga caaccgttca tggtcatgcg gtccataatc ctctcaccaa
     4981 gaagcgtgct cattgcggca tgattcaggt ttgttaacat cccgacactg cacagtgatg
     5041 ctgtccggcg atcaattatc tggtgcaata ccacctgctc gtttttcgtc tcccgctgaa
     5101 tgcctatttc atccaggacc agcaaatcaa ccccgcaaag ctcctgtaaa aatttttcac
     5161 cggatttgcc gttgtcgtag ctgtcatgca acacgctcat gacatcagac tcggtgacga
     5221 taatcacgct gcgccccttc gccatcagcc ggttacccat cgccgctgca aggtgatttt
     5281 tcccggtgcc ggttttaccg ctgaacacaa aattcgtgca cccggtcatc agttcgtcag
     5341 ctatggattt ggcctggctc agcgcgtatt tttgcccgtc gttctgcacc tgataattcg
     5401 caaacgagca tttgctgtgc agaggctgga tgcccgaacg attcaggatt ttttccaccc
     5461 gcaactggcg attctggcgg ttaatctcct cgctgcgttt tcgtccttca gcaagttgcc
     5521 attcccgcca ctcctccacc gtccggtacg gtggaaccgc cccctgtggt gcaagtctgt
     5581 gaatacgttc aagaaccccg gctgccgcaa tgtttttcat gccacatcac cccctgaatc
     5641 ccggcggaat ttcggtatcc ggttcagaaa tatgattcac acaacgctgg ttgttcgtgc
     5701 cgcttaccgg gagcaaccag gggttttcaa aattccggtc cggcccaaaa aacgtcgtcg
     5761 ctcgctgaac aaattccgtt cccgctttcc cggtcgccgc caggtatctc gcgtaacgcc
     5821 tcacaccatc cagcatggtc tctggtggca ccccctcgcg caatctggcc ttccaggcac
     5881 tgaatgcgga tttcttcggg tttgccccgg cacgcaacgg gtattcccgc cagacctgtt
     5941 cgaacacatc cggataatcc actcgtccca caggctgccc ggtgttttcc ggggctaccc
     6001 gatcggcttc ccgctgaatg gcggaatcgg cttcaggctg ctgcagttgg agtgattgct
     6061 ccggccttgc ggtcatcacc tgctgcacag cgcccgaatc ggctttcagc gcatacgctg
     6121 aatcggcttc cggtgtcgag cctgctggct gaccaggatt gacggtctga acatcccctg
     6181 cctggttcgt ggcgtttttt acgccatgga ccatagtgtt ttgatcttct ttatctgtat
     6241 ctttatctgt atctgtcgtg actcgtcgtg acatgtgcgt gacatttcgt gacgcgccgt
     6301 gacaatcgcc attttgttcc cgctttcttt ccctctctcg ctgcgccctc ttgcgctctg
     6361 caggagattt tgcggtttgc gaaatattgc cgttgtcctc tttaagcacc tggcgttttt
     6421 cccatccagt gattaaatca ccatcaagta cccgcccctg catcgtctgc aaaattgaat
     6481 caattacctc ttctgtcacg tcgagcgcac ttgccaaatc ttctgtcgtg acatcaatgt
     6541 gacctcgcgt gacatttcgt gacgcgctca ccaggaggtg gatatacact gccatcactg
     6601 ttgcaattgg ctgccctgac accctggcaa ttgttcgcca cttagggtca tttggcatgt
     6661 catgccataa tctgagccag gcgttagcca tactcacctc ttctgatacc gaatcttttt
     6721 actcacgagt tgccgggagc gattcgatat ggctattgtc agtcaatgta ctgccacagc
     6781 atttcctgcc gggccaccac ggttcatctg attgaaaccg gcgattgcca ctgcgacaaa
     6841 atcatcagcg tctctcacca gtcgttcatg cgtctccacc agttcccgaa aataagctga
     6901 actgtggctg cgcattcgag ccaccagcgg aggtggcatt gccctttcga tcgcaggtaa
     6961 caacgcctga attttttcaa cagcgtcagg ggtgtcttta tccagccaac ggaaaatctt
     7021 ttgggtgttg agataaagcg tgttgtgctt gctgtcgtta tgcagaaccg gaaacgtcat
     7081 tcccagttcg aaataaaccc tggttatttc agctgccgga actttttcac cgtccggatg
     7141 cgcccaggct ttcatcgcca tgcggatgtg ttcatgcttg attttcatga atcaaccccc
     7201 gccgatggtt gtgtgttagc ctgaaactca gcaggcaagc cgtcagttgg gttggggtag
     7261 gttttactgt caatctcatg tggggtcacc acccatcccg ttgcttcaca ccaacgtaat
     7321 attttttctc ctgtaagttt tgcacgcccc gttataacgt ggctgaccat cccttgggat
     7381 gcgccaacaa tctcagcaaa tgaactctgg gttatatgaa aatgatgcaa atattctcca
     7441 agattcataa tccgccctat gtgatgtgtt accaatgcat aattaatagc actgttattt
     7501 ttaaaagtaa atagtgatac tatttcaaag tgattagcaa tcttattaga attggatgta
     7561 tgaaaagaaa atcattgtca gacaccgacc tcaacgccgc cagaagactg aaagaaatct
     7621 ggatggcaaa gaaatctcag ttgggattga ctcaggaacg tgcggcggaa attatggggt
     7681 tttcaacaca aggggcggta agtcactacc taaatggtca aaccccacta aaccttgagg
     7741 ctgttctaaa gtttgctggc ttactgaaag ttccccctga gtcaatcagg ccagacatgg
     7801 tagatttgct gcaaattgca aggatgtatt gtcgagaacc acagcaagat aacatagtcg
     7861 cattaccagc agacacagaa caaacagagg atgaactacc gtttgccgta gagccgatgg
     7921 aacgagattt aatccatacg ttcagagctt ttccgaaaga agatcaagaa cacatgcttc
     7981 aggagatgaa agataaaaaa gaatcaatgg atcgtacggt tgcgcggtgg ttagctgcac
     8041 aaaaaggccg tcgcgcctaa atcagaggag atatgtaaaa tgagtacagt cctttcccca
     8101 atggtttcag aatttgagac agttgagcaa gaaaatagtt acaacgaatg gctgcgggcc
     8161 aaagtagcag caagcctcgc ggatccccgc ccggcgattc cgcatgatga ggtaatggcg
     8221 gaaatggaac aaattattgc ccaaattgct gctgcaactc acagaggtga ataatgttac
     8281 ccgttttatg gctaccatct gctcgtgatg atttgcgcca gatcgtagcc tacatagcta
     8341 aagaaaatcc cgcggcagca cgcagaatga aaattcgaat tgaaacatcc gttttgccgc
     8401 tcacagagca tccttacctg tatcctccaa gcgagcgagt tcttggtctt cgtgaaattg
     8461 taactcatcc taactacatc atcctttacc gagtaacagc atcaagcata gaagtagtaa
     8521 atattgtgca ttctcgaaga cagtatccag gcaaaaacag ttaattattc ccgccgacag
     8581 aaccacctta gggtggtttt ttcttgcccc aacaaaaaac aatagcgatg ctatttacat
     8641 caaaaaaaat aatgatgcta ttgtttcagg cgtcaaccca ccccgcccca cagaatgcag
     8701 ggcaatactt cgagttacca gacagtggtc aggggttaag tagccagccc gaggcgtaag
     8761 aacatgacgg cagggttcaa tctttaacta tgcagcaggt tttttgttcc gccaccccgg
     8821 cgttaagggg aaatgaggtc agcatggata ctatcgatct tggcaacaac gaatctctgg
     8881 tgtgcggcgt gttccctaac caggatggaa cgtttaccgc catgacgtat accaaaagca
     8941 aaacgtttaa aaccgaatct ggcgcgcgtt gctggttagc cagaaacact gactgatgag
     9001 gctgacgatg gaatttaaag atttaccgtc tgatgtacag aaaacagcag ctcatacatt
     9061 gcattctgtg ctgcgagaaa tcgggaaaga tattgcaagc gagccagcaa aagatctggc
     9121 ccggaaaatc aagaccgctt tcgttgagct ttataatgtt ggcactgact ctgaaactgt
     9181 cgagaccaag accgtaagtt caccaatatt ctcacttgag ccaagcgtat tatcaggtga
     9241 aatatgcacc gagatttcca gcgaactgct tccggtaata cgagaagcaa ttcgccgtcg
     9301 cggtttggat ggaagttatg atcatgacgt tctgcaagtt ctcaggacaa tggtgacttc
     9361 actggggatt tgatccctgc gtcctctaag cttttgatat agcggtcctc caagaagcgg
     9421 taaattttgt cgaaatcatc ttttccctct ggtttattca gaggattaca ggacccgtaa
     9481 gtagcggcat acatttcaac c
//
LOCUS       DADMKF010000148         9439 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.151, whole genome shotgun sequence.
ACCESSION   DADMKF010000148 DADMKF010000000
VERSION     DADMKF010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9439
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.151"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            <1..173
                     /locus_tag="JNC24_003321"
     CDS             <1..173
                     /locus_tag="JNC24_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAY9159998.1"
                     /translation="RNRRLDFCADAIRHAADDEKLAGIGFHWGFSDQSHFSTVFKQRF
                     GMTPGEYRRKFR"
     gene            complement(345..674)
                     /locus_tag="JNC24_003322"
     CDS             complement(345..674)
                     /locus_tag="JNC24_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HAY9159999.1"
                     /translation="MMKKTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVA
                     LKTDAETEKLVTDINAERKASYQQLAKQNNVSVDDIAKLAGQKLVARAKPGEYVQGIN
                     GKWVRKF"
     gene            complement(679..864)
                     /locus_tag="JNC24_003323"
     CDS             complement(679..864)
                     /locus_tag="JNC24_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HAY9160000.1"
                     /translation="MKILLAALTSSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(861..3500)
                     /locus_tag="JNC24_003324"
     CDS             complement(861..3500)
                     /locus_tag="JNC24_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HAY9160001.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLAGIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGMVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVIAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWPYAGFPLS
                     GRLGVKVDNWQAGLENALISGRLSVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLTDPTLAFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHEYELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPINATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRDPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGPMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTKINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
     gene            3708..4697
                     /gene="ldhA"
                     /locus_tag="JNC24_003325"
     CDS             3708..4697
                     /gene="ldhA"
                     /locus_tag="JNC24_003325"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HAY9160002.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            4808..5230
                     /gene="hslJ"
                     /locus_tag="JNC24_003326"
     CDS             4808..5230
                     /gene="hslJ"
                     /locus_tag="JNC24_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HAY9160003.1"
                     /translation="MKKVAAFVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     NTISEMLKEGAQVDLTANQLTLATAKQTLTYKLADLMN"
     gene            complement(5227..5493)
                     /locus_tag="JNC24_003327"
     CDS             complement(5227..5493)
                     /locus_tag="JNC24_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015964583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HAY9160004.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPGLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            5767..9291
                     /gene="nifJ"
                     /locus_tag="JNC24_003328"
     CDS             5767..9291
                     /gene="nifJ"
                     /locus_tag="JNC24_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HAY9160005.1"
                     /translation="MITIDGNGAVASVAFRTSEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPCVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFSAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQTLPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGKRETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTQPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPGG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARKCGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRLEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACSGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAADLQKRYDFLAQMAGKAEKSNTD"
BASE COUNT         2349 a         2466 c         2308 g         2316 t
ORIGIN      
        1 ttcgtaaccg tcgtctcgat ttttgtgcag atgcgattcg ccatgccgca gatgatgaaa
       61 aactggcagg catcggcttt cattggggat tttctgacca gagtcatttt tcaacggtat
      121 ttaagcaacg ctttgggatg acgccaggcg agtatcgacg taaattccgt taactaattc
      181 gcgttgtgtg tactgcaacg ccaattagtt aaaacagata tttgactatg catttttcag
      241 ggcaaaaggc aatttgacag gagtttttga atattcacct tgtttcagga aaatagatca
      301 atcgctttct cctgtagcgt tatcccgata ttgcggtttt gctattaaaa ttttcgcacc
      361 catttaccgt ttatcccttg tacatattcc cccggtttgg cccgagctac gagcttttgc
      421 ccggcgagtt tcgcgatatc atctaccgac acattatttt gctttgccag ttgttggtaa
      481 ctcgctttac gttcggcatt aatgtcggtt actaatttct ctgtttcagc atccgttttc
      541 agcgcaacca gataaccata aaatgtttca cctacccgcc cctgagttct ggcttcatcc
      601 agagtcaatg ccattacatt gctgcttacc agcccaacaa gaaacgcaca aagaagtaat
      661 gttttcttca tcatcgcctc agaaaagatc gctacgagtt tcaagcagct cttcgacatc
      721 tttgtctgcc ttgatgatga tctcatgctc aattttaacg ttcatattga tagtgatcgg
      781 ttccttgggc gcagcgactt caatacgcgg cgtacagcca accagcataa aagatgacgt
      841 caacgcagcc agtaaaattt tcattgtttt tcctcacact cttttccttg cggacagtca
      901 tttcccggca gacgtgcgtt ctgctccaac catgcctgga gattatcgcc aaagcgtaaa
      961 ctgcgccaca gcgtaaaaat attctcttca tgatggtaat taaggtttac cgtaccgctt
     1021 ttaccatcaa cgcgactggt acctgtaatg ttggcctgca tggttaataa accgagatta
     1081 tctaagttaa tttttgtcga tgaccggcta atttccatat agcgcaacca gttaattgct
     1141 gaacccgcag tcatattgtc tttcaccacc gcatctgcgg tgtctttatc cagccgcaag
     1201 gtcattggcc cgctatttgc cagccaacca tctttcacta tccatttttc gttgtttagc
     1261 cataacggca atgcaccact aaatgccccg gacatggcga attgtttcgg attgacggcg
     1321 ctaaccagtt cgctggatga gagattattc agccgcaaca gcgccgggtc atgttgcggc
     1381 atacgtaatt gtttcatcag tacgttaccg cctaacacat cgacgctaac atcagtcaac
     1441 agcaagggtt cttcttcagt ccacggataa cgcccttgca aatcagccgt aatatttttc
     1501 gctgtcacca gattaatcac ttcggcaatt cgcaacgtaa cggggccgcg agtccccaga
     1561 tgccaggctc catcggcaaa acggaaaggc aggacaaaat cgacaccgtt aacctgatta
     1621 tctggcatcc aggcactacc gcctttcaac acgccgtgtc ctcccgcgcg gaatccttgt
     1681 tcaggcgcag cagaaaatgc aacctgagca tacagttccc catcgcgtaa gttcatcttc
     1741 cagtcgggtg gcaccagcgg ctggaataca gtcagtgatt gttttggcca ccaggcgtta
     1801 ccgcgcagac gaataccgtc ccagcgacca tttacccgaa ccgggccaat ctcaccagca
     1861 tgtaaatcgc ctttaaagag gaaatacgtc ggatcgcgcc catcgacgct aaattttaag
     1921 gttgatggcg gtaacacact gccgccagtg aacagcgttt gcccggcgtc cagtgacagc
     1981 gcgccgctga aggagggatg ctgtgcgtca cgtacccaga cgacgggctt gtcgagaatt
     2041 aatcgcggct tttctaccgt catcgtaccg tattgtaact ggtcgaaacc ggtggagagg
     2101 tcggtcagcg taatcgtgct gtcatgccat tcaccttttc ctgcgacatc ccaggtggca
     2161 tttatcggtg taaaactgcc ttttccccag tagcgccact gccagcggcc cgcgtcaggg
     2221 agaaaatcat tcgccagccc atccatatgc agcacgaaat cgcccagttc atattcatgc
     2281 gcctgcaaaa tggcctgcaa acgtccgtca acaccacgtt gggtgacttt tacacccgct
     2341 aaaggccagc ggatttcatc gatgtccagc gaatcgatga ctcttccctt cgaacgtaat
     2401 aacgcgcctg gctcaaaggc cagcgttggg tcagtcagac ttccacttag ctgcgcgggt
     2461 aagcgggcat ataaaatgag gtccgcctgt ttcgcttcac cggtcagctg cagaggcagc
     2521 tggctgttat ccatgcttaa ttttcctggg ccaaaattta gcaccgcgtt gcctttaccc
     2581 gcttgcccct gggtcagcac actcagtcgt ccgctgatca gagcgttctc aagccctgcc
     2641 tgccagttgt cgactttgac acccagtcgg ccactcaaag gaaaacctgc atacggccaa
     2701 ctccagcgac catcgcttac ggtcaattgt tgacgagtaa tttgccacgg caaatcgagc
     2761 aacggatcgc cgttatcccg tgccagcaca atcaattgcc cgctattttc ctgccagtcc
     2821 agctctgcat ccaccagtga cggttcctgc ggcaagttta acgtcgcagt agcatgccca
     2881 cttacaggaa gtccatccgg cacgagcggc atagtaaatt cccccaccag tttaaccggc
     2941 ggctgatttt caaacgcgat gacatccagt tcgctgactg taagttgttg ccctttcagc
     3001 tggccttgaa atttaacttt ttcgccctga taacgcagtt gctgaatatc agaggttaat
     3061 gcgagagaga gttttccctg ccattcctgc cagggagaaa aaatcagttt atcgatattg
     3121 atccaggtgt taggcagcat ggactgccac tgcgcgaggg tttttggagc agctggcgat
     3181 tgctccgtct gcggcaattt cgccagacaa gcagaatcaa gttctaccat gccgacgttc
     3241 aataaccagc ggctgggatg tgaaaggctg gcgttggtga tatgcgcaag ctgacaatct
     3301 cccaccagat aacggagatc gggaatgatt aaacctttac gcgtaatgcg tgggctttca
     3361 tctaatgcaa tacgtgtccc gagcggtagc cagatgcccg ccagcgtggg aacccacagc
     3421 ccgagcgtca tcagcagcgt caacggcaca agaataatca gtaataacag cgcgagaacg
     3481 gctttatatt tacccagcat gggtagttaa tatcctgatt tagcgaaaaa ttaagcattc
     3541 aatacgggta ttgtggcatg tttaaccgtt cagttgaagg ttgcgcctac actaagcatt
     3601 gttgttgatg aatttttcaa tatcgccata gctttcaatt atatttgaaa ttttgtaaaa
     3661 tatttttagt agcttaaatg tgattcaaca tcactggaga aagtcttatg aaactcgccg
     3721 tttatagcac aaaacagtac gacaagaagt acctgcaaca ggtgaacgag tcctttggct
     3781 ttgagctgga attttttgac tttctgctga cggaaaaaac cgctaaaact gccaatggct
     3841 gcgaagcggt atgtattttc gtaaacgatg acggcagccg cccggtgctg gaagagctga
     3901 aaaagcacgg cgttaaatat atcgccctgc gctgtgccgg tttcaataac gtcgaccttg
     3961 acgcggcaaa agaactgggg ctgaaagtag tccgtgttcc agcctatgat ccagaggccg
     4021 ttgctgaaca cgccatcggt atgatgatga cgctgaaccg ccgtattcac cgcgcgtatc
     4081 agcgtacccg tgacgctaac ttctctctgg aaggtctgac cggctttact atgtatggca
     4141 aaacggcagg cgttatcggt accggtaaaa tcggtgtggc gatgctgcgc attctgaaag
     4201 gttttggtat gcgtctgctg gcgttcgatc cgtatccaag tgcagcggcg ctggaactcg
     4261 gtgtggagta tgtcgatctg ccaaccctgt tctctgaatc agacgttatc tctctgcact
     4321 gcccgctgac accggaaaac taccatctgt tgaacgaagc cgccttcgat cagatgaaaa
     4381 atggcgtgat gatcgtcaat accagtcgcg gtgcattgat tgattctcag gcagcaattg
     4441 aagcgctgaa aaatcagaaa attgggtcgt tgggtatgga cgtctatgag aacgaacgcg
     4501 atctattctt tgaagataaa tccaacgacg tgatccagga tgacgtattc cgtcgcctgt
     4561 ctgcctgcca caacgtgctg tttaccgggc accaggcatt cctgacagca gaagctctga
     4621 ccagtatttc tcagactacg ctgcaaaact taagcaatct ggaaaaaggc gaaacctgcc
     4681 cgaacgaact ggtttaatct tgccgctccc ctgcattcca ggggagctta ttcagataat
     4741 ccccaaagac ctttcatctt ctattcttaa aatagccctg agtcagaaac tgtaattgag
     4801 aaccacaatg aagaaagtag ccgcgtttgt tgcgctaagc ctgctgatgg cgggatgtgt
     4861 aagtaatgac aaaattgctg ttacgccaga acagctacag catcatcgct ttgtgctgga
     4921 aagcgtaaac ggtaagcccg tgaccagcga taaaaatccg ccagaaatca gctttggtga
     4981 aaaaatgatg atttccggca gcatgtgtaa ccgctttagc ggtgaaggca aactgtctaa
     5041 tggtgaactg acagccaaag ggctggcaat gacccgtatg atgtgcgcta acccgcagct
     5101 taatgaactc gataacacca ttagcgaaat gctgaaagaa ggtgcacaag tggatctgac
     5161 cgcgaaccag ttaacgctgg cgaccgcgaa acagacatta acttataagc tggcggattt
     5221 aatgaattaa tagctgccac agctcccggc ggcaagtgac tgttcgctac agcgtttgcc
     5281 gttgggtaat gcacacatcc caatcgccgt accatccagt tgacgggcaa cagaaagcga
     5341 accgccgatc attgcacaat ttgcttctcc actactggac atcgacgctt ttaaacctgg
     5401 cgctacgtgc gccgcagtcg cctgctgaac aggttcacta ctacacgccg acaacaataa
     5461 agcggcacac cctacccaaa acgctgctcg catctcttct tcctctgatc ttcaagccaa
     5521 acgacaccgc cataaataat aggcagcaca gagggcggcg tcgagagctg tcctgcgcgt
     5581 tgccccgcat ttttactttt ttatggctat ttttttgccc tctgtttgat caaaacattc
     5641 attacgctga tgtgggggac acaaaagcga aaatgcagaa gaaagccatt tgctaaaatt
     5701 gaaggattac tactgggcgc gcagcaattt cgtgcgcccc tcatttgcgc aatgtaaggg
     5761 tgtcatatga ttactattga tggtaatggc gcggttgctt cggtggcgtt tcgcaccagt
     5821 gaagttatcg ccatctaccc tattaccccc agttccacga tggcagaaca ggctgatgcc
     5881 tgggccggaa acggcttgaa gaacgtttgg ggagacacac catgcgtggt tgaaatgcag
     5941 tcggaagcgg gtgctatcgc taccgtgcat ggcgctttgc agacgggtgc cctttcaaca
     6001 tcgtttacgt catcgcaggg tttgctgctg atgatcccga cactgtacaa actggcaggc
     6061 gaactcacac cgtttgtcct gcatgtagcg gcacgtaccg ttgccacaca tgcactctct
     6121 atttttggcg atcattccga cgtcatggcg gtgcgccaga cgggttgcgc gatgttgtgt
     6181 gcggcaaacg tccaggaagc gcaagacttt gctctcattt cgcacatcgc gacgctgaaa
     6241 agccgcgtgc catttattca tttctttgat ggtttccgca cgtcccacga aatcaacaaa
     6301 attgtcccgc tggccgatga cacgattctt gatctcatgc cgcaggccga aattgatgct
     6361 catcgcgccc gggcgctcaa cccggaacat ccggtgatcc gtggtacgtc cgccaatcct
     6421 gacacttatt tccagtctcg cgaagccacc aacccctggt acaacgcagt ctatgaccat
     6481 gttgaacagg cgatgaatga tttctctgcc gcgacaggtc gtcagtatca gccgtttgaa
     6541 tattacgggc atccgcaagc tgaacgggtg attatcctga tgggctcagc cattggcacc
     6601 tgtgaagaag tggttgatga attgctaacc cgtggcgaaa aagtcggcgt gctgaaagtt
     6661 cgcctgtacc gccccttctc agccaaacac ttgctacaaa ctctaccggg atccgtacgc
     6721 agcgtggcgg tactggacag aaccaaagaa cccggtgccc aggcagaacc gctctatctg
     6781 gatgtgatga ccgcactggc agaagccttt aataatggca agcgcgaaac tctgccccgt
     6841 gtcattggtg ggcgctatgg tctttcatcc aaagaatttg gcccggactg tgtactggcg
     6901 gtatttgccg agctcaacgc ggctaaaccg aaagcgcgct ttacggttgg tatttacgat
     6961 gatgtaacca atctgtccct gccgttgccg gaaaacaccc agccaaactc ggcgaaactt
     7021 gaagccttgt tttatggcct tggtagtgat ggcagcgttt ccgcgaccaa aaacaatatc
     7081 aagattatcg gtaattccac gccgtggtac gcacagggct attttgttta cgactccaaa
     7141 aaagcgggcg gcctgacggt ttctcacctt cgcgtgagcg aacagccgat tcgttccgct
     7201 tatctcattt cccaggctga ttttgttggc tgccaccagt tgcagtttat cgataaatat
     7261 cagatggctg agcgtttaaa acctggcggc attttcctgc tcaacacgcc gtacagcgca
     7321 gatgaagtgt ggtcgcgctt gccgcaagaa gttcaggccg tgttaaacca gaaaaaagcg
     7381 cgcttctatg tgattaacgc ggcgaaaatc gcccgcaaat gtggcctggc ggcccgtatt
     7441 aataccgtca tgcagatggc ttttttccat ctgacgcaaa ttctgcctgg cgatagcgcc
     7501 ctcgcagaat tgcagggtgc gattgccaaa agttacagta gcaaaggcca ggatctggtg
     7561 gaacgcaact ggcaggctct ggcgctggcg cgtgaatccg tagaagaagt tccgttgcaa
     7621 ccggtaaatc cgcacagcgc caatcgaccg ccagtggttt ccgatgccgc ccctgatttc
     7681 gtgaaaaccg taaccgcagc gatgctcgca gggcttggtg atgccctccc cgtttcggcg
     7741 ctgccgccag acggcacctg gccgatgggc accactcgct gggaaaaacg caatatcgcc
     7801 gaagagatcc ccatctggaa agaggaactc tgtacccaat gtaaccactg cgtcgcagct
     7861 tgcccacact cagctattcg cgcaaaagtg gtaccacccg aagcgatgga aaacgcccct
     7921 gccagcctgc attcgctgga tgtgaaatcg cgtgatatgc gcgggcagaa atatgtcttg
     7981 caggtggctc cggaagattg caccggttgt aacctgtgcg tcgaagtttg cccggcgaaa
     8041 gaccgtcaga atccagagat taaagccatc aatatgatgt cgcgcctgga acatgtcgaa
     8101 gaagagaaaa tcaattacga tttcttcctc aacctgccag aaatcgaccg tagcaaactg
     8161 gaacgtattg atattcgtac atcgcagctg attacaccgc tgtttgaata ttccggtgct
     8221 tgctccggtt gtggcgagac gccgtatatt aaattgctga ctcagctcta tggcgaccgg
     8281 atgttgatcg ctaacgccac cggctgttct tcaatttatg gcggtaacct gccctctaca
     8341 ccgtatacca ccgatgccaa cggtcgtggg ccggcatggg cgaactctct atttgaagat
     8401 aatgccgaat ttggccttgg tttccgcctg acggtcgatc aacaccgtgt ccgcgtgctg
     8461 cgtctgctgg atcaatttgc cgataaaatc ccggcggaat tactgacggc gttgaaatca
     8521 gacgctacgc cagaggttcg tcgagaacag gtcgcagctt tacgccagca actcaacgat
     8581 gttgccgaag cacatgaact gctacgtgat gcagatgcac tggtggaaaa atcaatctgg
     8641 ctgattggtg gtgatggctg ggcttacgat atcggctttg gcggtctgga tcatgtattg
     8701 agtttgacgg aaaacgtcaa cattctggtg ctggatacgc aatgctattc caacaccggt
     8761 ggtcaggcgt cgaaagcgac accgctgggt gcagtaacta aatttggcga gcacggcaaa
     8821 cgtaaagcgc gtaaagatct tggcgtcagt atgatgatgt acggtcatgt ttatgtggcg
     8881 cagatttctc tcggcgcgca gctgaaccag acggtgaaag cgattcagga agcggaagcg
     8941 tatccgggac catcgctgat cattgcttat agcccgtgtg aagagcatgg ttacgatctg
     9001 gcactcagcc atgaccagat gcgccaactc acagctaccg gcttctggcc gctatatcgc
     9061 ttcgatcctc gtcgtgccga tgaaggcaaa ctgccgctgg cgctggattc acgcccgccg
     9121 tcagaagcac tggaagaaac gttacttcac gagcaacgtt tccgtcgtct gaattcgcag
     9181 cagccagaag tggcagaaca gttatggaaa gatgctgcag ctgatttgca aaaacgctat
     9241 gacttcctgg cacaaatggc cggaaaagcg gaaaaaagca acaccgatta aatgctctgg
     9301 ataaggatta cccaattcta aaaaaaagcc cggacgactg ttcgggcttg tctttttata
     9361 tgttggaaaa tcagtggcaa tgcaatggcc caacagaaaa taaaatctgt gacaataaag
     9421 gcatataacc cgcgcagaa
//
LOCUS       DADMKF010000149         9433 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.152, whole genome shotgun sequence.
ACCESSION   DADMKF010000149 DADMKF010000000
VERSION     DADMKF010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9433
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.152"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            206..1072
                     /gene="folD"
                     /locus_tag="JNC24_003329"
     CDS             206..1072
                     /gene="folD"
                     /locus_tag="JNC24_003329"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HAY9160006.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            1074..1286
                     /gene="ybcJ"
                     /locus_tag="JNC24_003330"
     CDS             1074..1286
                     /gene="ybcJ"
                     /locus_tag="JNC24_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HAY9160007.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKADGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            1394..1915
                     /locus_tag="JNC24_003331"
                     /pseudo
     CDS             1394..1915
                     /locus_tag="JNC24_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415060.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
     gene            complement(1951..3336)
                     /gene="cysS"
                     /locus_tag="JNC24_003332"
     CDS             complement(1951..3336)
                     /gene="cysS"
                     /locus_tag="JNC24_003332"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HAY9160008.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYAADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTEKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            3510..4004
                     /gene="ppiB"
                     /locus_tag="JNC24_003333"
     CDS             3510..4004
                     /gene="ppiB"
                     /locus_tag="JNC24_003333"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HAY9160009.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVEGMDVVDKIKGVATGRSGMHQDVPKEDVIIESV
                     TVSE"
     gene            4007..4729
                     /gene="lpxH"
                     /locus_tag="JNC24_003334"
     CDS             4007..4729
                     /gene="lpxH"
                     /locus_tag="JNC24_003334"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HAY9160010.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDEPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            4847..5356
                     /gene="purE"
                     /locus_tag="JNC24_003335"
     CDS             4847..5356
                     /gene="purE"
                     /locus_tag="JNC24_003335"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HAY9160011.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAAEIFEILNVLHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNDWRKAQTDEVL
                     ENPDPRGTA"
     gene            5353..6420
                     /gene="purK"
                     /locus_tag="JNC24_003336"
     CDS             5353..6420
                     /gene="purK"
                     /locus_tag="JNC24_003336"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HAY9160012.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWRLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYTLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVIWAQSKFS"
     gene            complement(6557..7450)
                     /locus_tag="JNC24_003337"
     CDS             complement(6557..7450)
                     /locus_tag="JNC24_003337"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HAY9160013.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRGKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(7447..8100)
                     /locus_tag="JNC24_003338"
     CDS             complement(7447..8100)
                     /locus_tag="JNC24_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HAY9160014.1"
                     /translation="MTIIHPLLASRSAPNYRQSWRLAGVWRRAINLMTESGELLTLHR
                     QGSGFGPGGWVLRRAQFDALCGGLCGNERPQVVAQGIRLGRFTVKQPQRYCLLRITPP
                     AHPQPLAAAWMQRAEETGLFGPLALAAGDPLPAELRQFFAYSDNLQLVTTAVSVSYLR
                     YAAQGYFASPLLHFVHALSCPKRTAVAIDSLLALGHTSGADTLLGFWLGQQLLQGKP"
     gene            complement(8111..9370)
                     /locus_tag="JNC24_003339"
     CDS             complement(8111..9370)
                     /locus_tag="JNC24_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009029994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HAY9160015.1"
                     /translation="MFTSVAQANAAVIEQIRRARPHWLDVQPASSLISELNEGKTLLH
                     AGPPMRWQEMTGPMKGACVGACLFEGWAKDEAQALAILEQGEVNFIPCHHVNAVGPMG
                     GITSASMPMLVVENVTDGNRAYCNLNEGIGKVMRFGAYGEDVLTRHRWMRDVLMPVLS
                     AALGRMERGIDLTAMMAQGITMGDEFHQRNIASSALLMRALAPQIARLDHDKQHIAEV
                     MDFLSVTDQFFLNLAMAYCKAAMDAGAMIRAGSIVTAMTRNGNMFGIRVSGLGERWFT
                     APVNTPQGLFFTGFSQEQANPDMGDSAITETFGIGGAAMIAAPGVTRFVGAGGMEAAR
                     AVSEEMAEIYLERNMQLQIPGWDFQGACLGLDIRRVVETDITPLINTGIAHKEAGIGQ
                     IGAGTVRAPLACFEQALEALAESMGIG"
BASE COUNT         2102 a         2673 c         2498 g         2160 t
ORIGIN      
        1 ttagacaagg aaataagtca gttccagatt tacaacgcca tcatggacga aaaatgaagc
       61 tttcaatctc agcgacggtg cgccccacct tcgcaagagg tcgcttcacg cgataaatct
      121 gaaacgaaac ctgacagcgc gccccgcttc tgacaaaata ggcgcatccc cttcgaccta
      181 cgtaacagat ggaatcctct ctctgatggc agcaaagatt attgacggta aaacgattgc
      241 gcagcaggtg cgctctgaag ttgctcaaaa agttcaggcg cgtattgcag ccggactgcg
      301 ggcaccagga ctggccgttg tgctggtggg tagtaaccct gcatcgcaaa tttatgtcgc
      361 aagcaaacgc aaggcttgtg aagaagtcgg gttcgtctcc cgctcttatg acctcccgga
      421 aaccaccagc gaagcggagc tgttggagct tatcgatgcg ctgaatgccg acaacaccat
      481 cgatggcatt ctggttcaac tgccgttacc ggcgggtatt gataacgtca aagtgctgga
      541 acgtattcac ccggacaaag acgtagacgg tttccatcct tataacgtcg gtcgcctgtg
      601 ccagcgcgcg ccgcgtctgc gcccctgcac ccctcgcggc atcgtcacgc tgcttgagcg
      661 ttacaacatt gataccttcg gcctcaacgc cgtggtgatt ggcgcatcga atatcgtcgg
      721 tcgtccaatg agcatggaac tgttgctcgc aggttgcacc actaccgtga ctcaccgctt
      781 cactaaaaat ctgcgtcacc acgtagaaaa tgccgatcta ctgatcgttg ccgtcggcaa
      841 gccgggcttt attcctggtg actggatcaa agaaggcgca attgtgattg atgtcggcat
      901 caaccgtctg gaaaatggca aagttgtggg cgacgtcgtg tttgaagacg cggctaaacg
      961 cgcctcatac attacgcctg ttccgggcgg cgttggcccg atgacggttg ccacgctgat
     1021 tgaaaacacg ctacaggcgt gcgttgaata tcatgatcca cagggtgagt aacatggcga
     1081 cattttcttt aggtaaacat ccgcacgttg agctgtgcga cttgctgaaa ctggaaggct
     1141 ggagcgaaag cggcgcgcag gcgaaaatcg cgattgccga aggccaggtg aaagccgacg
     1201 gcgcggttga aacgcgcaaa cgctgcaaaa tcgtcgccgg tcagacagtg agttttgcag
     1261 gtcacagcgt acaggttgtt gcctgatccc tttcccggcc tgtatcaaca ggccggttct
     1321 cttttcagac ttttgccctc tacgccccgt tcatccatgc tgatagcatc gacattttgc
     1381 cgaagcgacc atcatgccaa ccgttattac gcacgccgcc gttcccctat gcattggttt
     1441 agggttgagt tcgaaagtca tccctccgcg tctgttattt gcctgaatca tcctggcgat
     1501 gctgcccgac gccgacgtat tgtcgtttaa atttggcgtt gcttacggca atgtttttgg
     1561 tcatcgcggg tttacccatt cgctggtgtt tgcgtttgtt gtcccgctgt tatgtgtgct
     1621 gattggacgc cgatggttca gggctgggct gattcgctgc tggctgtttt taaccgtctc
     1681 gttgctatcg cacagcatgc tggattcggt aaccactggc ggtaagggtg ttggctggct
     1741 gtggccgtgg tcagatgaac gctttttcgc tccctggcag gtgattaaag tcgcgccgtt
     1801 tgcattgtct tgttacacca cgccgtacgg gcatcaggtg attatttctg aattgatgtg
     1861 ggtgtggttg ccggggatgc tactgatggg aatgttgtgg tggcgcagac gataaccgga
     1921 tgcgaaaact cgcatccggc aatagcgcaa ttacttacga cgccaggtgg tcccttgcgg
     1981 gccatcttcc agaacgatcc ccatctcgtt aagacggtca cgcgccgcat ctgccgccgc
     2041 ccagtctttc gctttacggg catccagacg ctgttgaatt aacgcttcaa tctcagccac
     2101 ttcgctgtcg tctgcctgcg cgccgctttg caggaacgct tccggttctt gctccagcag
     2161 gcctaataca gcggaaagtt tacgcaggtg agatgccatt gcattcgccg ctgccatatc
     2221 ttctgctttc agacggttta cttcacgcgc catatcaaac agtacggaat aggcttccgg
     2281 ggtgttgaaa tcgtcgtcca tcgcttcaat aaagcgcgct tcaaacgctt cgccaccggc
     2341 aggagcaacg gttttttctg tgccgcgcag ggcagtgtag agacgctcca gcgccgcacg
     2401 cgcctgcttc aggttctctt cgctgtagtt cagctggctg cgatagtggc ccgacatcag
     2461 gaagtaacgc acggtttcag catcgtagta tttcagcaca tcgcgcacgg taaagaagtt
     2521 acccagcgat ttggacatct tctcgcggtc aaccatcacc attccggagt gcatccagta
     2581 gttaacatac tgaccatcat gggcacaggt ggactgcgcg atttcattct cgtggtgcgg
     2641 gaacatcagg tcagaaccgc cgccgtggat atcaaagtgg ttacccagct gcttgcagtt
     2701 cattgccgaa cattcaatgt gccagcctgg acggcccgcg ccccacggag acggccagct
     2761 cggttcgccc tctttcgaca tcttccacag aacgaagtcc atcgggttgc gtttgtcgtc
     2821 gaccacgtca acgcgcgcgc ctgcctgcag ctggtcgaga tcctgacgcg acagcacacc
     2881 ataagtcgga tcggtcggga cgtcgaacat cacgtcgccg ttgtccgccg cataagcgtg
     2941 acctttggcg atcagttgtt cagtgagttc aataatttct gcgatatggt gcgtcgcgcg
     3001 cggctccata tccgggcgca gaatgttcaa agcatcaaaa tctttgtgca tttcggcgat
     3061 catgcgatcc accagcgcca caaagctttc gccattttca ttggcacgtt tgatgatttt
     3121 gtcgtcgata tcggtaatgt tgcgcacata cttcagttta tagccgagga aacgcagata
     3181 gcgcgcaacc acgtcaaaag caacaaaggt acgcccgtga ccaatatgac agagatcgta
     3241 aacggtgatt ccacacacat acatgccgac ttccccggcg tgaataggct taaattcctc
     3301 tttttggcgt gtcagagtat tgaagatttt tagcatcgaa gattccgttt agacatgtgt
     3361 gggtaattga gttgcgtata atacccatat ttcccgcgcg aatcagcata cattgcgtga
     3421 tgatccgatc gtgcggttat gctataacac caccctatat atgacccgaa ctgggttgaa
     3481 gcaccaatca aacggaacag gatgcaaaaa tggttacttt ccacaccaat cacggcgata
     3541 ttgtcatcaa aacttttgac gataaagcac ctgaaacagt taaaaacttc ctggactact
     3601 gccgcgaagg tttttacaac aacaccattt tccaccgtgt tatcaacggc tttatgattc
     3661 agggcggcgg ttttgaaccg ggcatgaaac aaaaagccac caaagaaccg atcaaaaacg
     3721 aagccaacaa cggtctgaaa aatacccgtg gtacgctggc aatggcccgt actcaggctc
     3781 cgcactctgc aactgcacag ttcttcatca acgtggttga taacgacttc ctgaacttct
     3841 ctggcgaaag cctgcaaggt tggggctact gcgtgtttgc tgaagtggtt gaaggcatgg
     3901 acgtggtaga caaaatcaaa ggtgttgcaa ccggtcgtag cggtatgcac caggacgtgc
     3961 caaaagaaga cgttatcatt gaaagcgtga ccgttagcga gtaatcgtgg cgacactctt
     4021 tattgcagat cttcatctct gcgtggaaga accggcgatc accgccggtt ttctgcgttt
     4081 tttagcgggg gaagctcgca aggccgacgc gctgtatatt cttggcgatc tgtttgaagc
     4141 gtggattggc gacgacgagc ccaatccact ccatcgccag atggcggcag cgatcaaagc
     4201 ggtgtccgat tctggcgttc cctgttattt cattcatggc aaccgtgatt ttctgctcgg
     4261 caaacgcttt gcccgtgaaa gtggcatgac gttattgccg gaagaaaagg tgctcgaact
     4321 ttatggccgc cgggtgttga ttatgcatgg cgacacgctg tgcaccgatg acgcgggtta
     4381 tcaggctttt cgcgccaaag tccacaaacc ctggctgcaa atgctattcc tcgccctgcc
     4441 gttgtttgtg cgcaaacgca ttgccgcgcg aatgcgagcg aacagtaaag aagccaacag
     4501 cagtaaatcg ctggcgatca tggacgttaa ccaaaacgcg gtagtcagtg cgatggaaaa
     4561 acatcaggtg caatggctga tccacggaca tacccatcgc ccggcggtac atgaacttat
     4621 cgccaaccag caacctgctt ttcgcgtggt actcggtgcc tggcatacgg aaggttcgat
     4681 ggtgaaagtc acggcggatg acgttgagct gattcatttt ccgttttaaa aaacccgcaa
     4741 ctttgctgat ttcacagcca cgcaaccgtt ttccttgctc tctttccgtg ctattctctg
     4801 tgccctctaa agccgagagt tgtgcaccac aggagtttta agacgcatgt cttcccgcaa
     4861 taatccggcg cgtgtcgcca tcgtgatggg gtccaaaagc gactgggcta ccatgcagtt
     4921 cgccgccgaa atcttcgaaa tcctgaatgt cctgcaccac gttgaagtgg tttctgctca
     4981 ccgcaccccc gataaactgt tcagcttcgc cgaaagcgcc gaagagaacg gttatcaggt
     5041 gattattgcg ggcgcaggtg gcgcagcgca cctgccaggc atgattgccg ccaaaacgct
     5101 ggtgccggtg ctgggcgtgc cagtacagag cgccgcactg agcggtgtcg atagcctcta
     5161 ctccatcgta caaatgccgc gcggcattcc ggtgggtacg ctggcgattg gtaaagctgg
     5221 cgcggcaaac gcggcattac tggcagcaca aattctcgcg actcacgata aagagctaca
     5281 ccagcgtctg aatgactggc gcaaagcaca aactgatgaa gtgctggaaa acccggaccc
     5341 gcgaggtacg gcatgaaaca ggtttgcgtc ctcggtaacg ggcagttagg ccgtatgctg
     5401 cgtcaggcag gcgaaccgtt aggcattgct gtctggccag tcgggctgga cgctgaaccg
     5461 gcggcggtgc cttttcaaca aagcgtgatt accgctgaga tcgaacgctg gccggaaacc
     5521 gcattaaccc gcgagctggc gcgccatccg gcctttgtga accgcgatgt gttcccgatt
     5581 attgctgacc gtctgactca gaagcagctt ttcgataagc tccacctgcc gaccgcgccg
     5641 tggcagttac ttgccgatcg cagcgagtgg cctgcggtgt ttgatcgttt aggtgaactg
     5701 gcgattgtta agcgtcgcac tggtggctat gacggtcgcg gtcaatggcg tttacgcgcc
     5761 aatgaaaccg aacagttacc ggcagagtgt tacggcgaat gtattgtcga gcagggcatt
     5821 aacttctctg gtgaagtgtc gctggttggc gcgcgcggct ttgatggcag caccgtgttt
     5881 tatacgctga cgcataacct gcatcaggac ggtattttgc gcaccagcgt cgcttttccg
     5941 caggccaacg cacagcagca ggcgcaagcc gaagagatgc tgtcggcgat tatgcaggag
     6001 ctgggctatg tgggcgtgat ggcgatggag tgttttgtca ccccgcaagg tctgctgatc
     6061 aacgaactgg ctccgcgtgt gcataacagc ggtcactgga cacaaaatgg tgccagcatc
     6121 agccagtttg agctgcatct gcgggcgatt accgatctgc cgttaccgca accagtagtg
     6181 aataatccgt cggtgatgat caatctgatt ggtagcgatg tgaattatga ctggctgaaa
     6241 ttgccgctgg tgcatctgca ctggtacgac aaagaagtcc gtccggggcg taaagtgggg
     6301 catctgaatt tgaccgacag cgacacatcg cgtctgactg cgacgctgga agccttaatc
     6361 ccgctgctgc cgccagagta tgccagcggc gtgatttggg cgcagagtaa gttcagttaa
     6421 gcaggtgctc atttgattgc cggatgcggc gtaaacgcct tatccggctt accgctccgg
     6481 tacccattgt aggcctgata agacgcgtca agcgtcgcat caggcatggt gcaccgattg
     6541 ccggatgctc tcatcactac agcgaaatac aggtccccgc ttcgcccgcc agcgtctctt
     6601 caattcgcga taacgcccca atccacgcgg gtttaccacg gcttctgaca taaccactca
     6661 ccgccgttac cttcggcccc atcgaaccat cggctttggc aaatggcgct aactcatccg
     6721 gtgtggcatg gcgaatggca cgttgctgcg gcgttcccca gttttcatat accgcatcag
     6781 catcggtgag gatcaccagt ccatcggcat taatctgctc ggcgagcaac gcagcggcga
     6841 gatctttatc aatcactgct tcactccctg ccccatcttc cgtcacaggc acacctccgc
     6901 cgccactgca aatcaccaca tgcccctctt tgagcaacaa ctcgatggct tcgctgtcga
     6961 gaattttacg cggttgcgga gacgccacca cccggcgcaa atatttacca tcacgtttca
     7021 tctgccagcc gtaagccgct tccagtgcct cttgttcttc tggctgataa accggaccaa
     7081 taaatttctc tggctgcaaa aacgccggat catcaggcga aacctcaatg cgcgtcagca
     7141 ccgtcgtaac aggcggcatc tgcggctgtg cgctcaagct ctgcgccagc atatagccaa
     7201 tcatcccctg gctttccgcc accagcacat ccagcggata cggttctacc tctttccacg
     7261 ccagattctg taacgccagc agccccacct gcggcccgtt gccgtgaaca atcgccagcc
     7321 gataagaacg ggccaggcgt gccagcgcgg gtacagcact ggcgatattg cgatattgat
     7381 tttccgccgt cagcgcctca ccgcgctgga gtaaggcgtt gcccccaaga gctacaacca
     7441 gtgttttcat ggttttcctt gtaataattg ttggccaagc cagaacccca gcagcgtatc
     7501 tgcccctgac gtatgcccca gcgccagcag cgaatcaatc gcaacagcgg tacgtttcgg
     7561 gcaactcaga gcatggacaa agtgcaggag tggcgaggcg aaatatcctt gcgcggcata
     7621 acgtaaataa ctgacgctca ccgctgtggt aacgagttga agattgtcgg aataggcaaa
     7681 aaactggcgt aactcagcag gcagcggatc gcctgccgcc aacgccagtg gcccgaaaag
     7741 cccggtttcc tccgcgcgtt gcatccatgc agctgcaagc ggttgaggat gcgcaggcgg
     7801 cgtaatacgc agcaaacaat aacgctgtgg ctgtttaacc gtgaaacgcc cgaggcgaat
     7861 cccttgagcc acaacctgtg gtcgttcatt gccgcataat ccaccgcata acgcatcgaa
     7921 ttgcgcgcgg cgaagcaccc atcctccggg gccgaaacca ctaccctgac gatgcagcgt
     7981 taacagttcg ccgctttccg tcatcaggtt aatcgcccgt cgccacactc ccgctaaccg
     8041 ccaggactgg cgataattcg gtgcactacg gctggcaagc agaggatgga tgatcgtcat
     8101 tgcgcgttcc tcaaccaatc cccatgcttt cagccagtgc ttccagcgcc tgttcaaaac
     8161 acgccagcgg tgcccgcaca gtgcctgcgc caatctgccc gatccccgcc tctttatggg
     8221 cgataccggt attgatgagt ggcgtaatgt cggtttctac cacgcgacga atgtccagtc
     8281 ccaggcacgc gccctgaaaa tcccagcctg ggatctgcaa ctgcatattg cgctcaaggt
     8341 aaatttccgc catctcttca gataccgctc tggccgcttc catgccaccc gcaccgacaa
     8401 agcgcgttac gccaggcgct gcgatcattg ccgcacctcc gataccaaag gtttcggtaa
     8461 tcgcgctatc gcccatatcc gggttcgcct gctcctgcga gaagccggtg aaaaacagac
     8521 cttgcggagt gtttacaggc gcagtaaacc agcgttcgcc cagcccgctt acccgaatcc
     8581 cgaacatatt gccgttgcgg gtcattgccg tgacgatgct gcctgcgcgg atcatcgcgc
     8641 cagcatccat cgccgccttg cagtaagcca tcgcgaggtt gaggaagaac tgatcggtca
     8701 cgctgaggaa atccatcact tcggcgatgt gctgtttatc atgatcgagg cgagcaattt
     8761 gtggggccag cgcacgcatt aacagtgcag aggaagcaat attgcgttga tggaactcat
     8821 cgcccatcgt aatgccctgc gccatcatcg ccgtgagatc gataccgcgc tccatgcgcc
     8881 ccagcgccgc gcttaatact ggcattaaca catcgcgcat ccagcgatgg cgagtcagga
     8941 catcttcgcc gtaagcgcca aaacgcatca ctttgccgat accttcgttg aggttgcagt
     9001 acgcccggtt gccgtcggtc acgttctcga ccaccagcat cggcatactg gcagaagtaa
     9061 taccgcccat tggaccgacg gcattcacat ggtgacaagg aatgaagttc acttccccct
     9121 gctccagtat tgccagcgcc tgcgcttcat ctttcgccca gccttcgaac agacatgcgc
     9181 ccacgcacgc ccctttcatg ggtccggtca tctcctgcca gcgcattggc ggcccggcgt
     9241 gaagcagtgt tttgccctcg tttagttcgc tgataagtga agaagccggt tgcacatcca
     9301 gccagtgtgg acgagcgcga cgaatttgtt cgataaccgc agcattggct tgcgccactg
     9361 atgtaaacat gggaacccct tattgcaaac gttctaataa acgagccagg taatgactcc
     9421 aacttattga tag
//
LOCUS       DADMKF010000150         9397 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.153, whole genome shotgun sequence.
ACCESSION   DADMKF010000150 DADMKF010000000
VERSION     DADMKF010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9397
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.153"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(350..1603)
                     /locus_tag="JNC24_003340"
     CDS             complement(350..1603)
                     /locus_tag="JNC24_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY9160016.1"
                     /translation="MSRTTTVDGAPASDTDKQSISQPNQFIKRGTPQFMRVTLALFSA
                     GLATFALLYCVQPILPVLSQEFGLTPANSSISLSISTAMLAIGLLFTGPLSDAIGRKP
                     VMVTALLLASICTLLSTMMTSWHGILIMRALIGLSLSGVAAVGMTYLSEEIHPSFVAF
                     SMGLYISGNSIGGMSGRLISGVFTDFFNWRIALAAIGCFALASALMFWKILPESRHFR
                     PTSLRPKTLFINFRLHWRDRGLPLLFAEGFLLMGSFVTLFNYIGYRLMLSPWHVSQAV
                     VGLLSLAYLTGTWSSPKAGTMTTRYGRGPVMLFSTGVMLFGLLMTLFSSLWLIFAGML
                     LFSAGFFAAHSVASSWIGPRAKRAKGQASSLYLFSYYLGSSIAGTLGGVFWHNYGWNG
                     VGAFIALMLVIALLVGTRLHRRLHA"
     gene            1710..2603
                     /locus_tag="JNC24_003341"
     CDS             1710..2603
                     /locus_tag="JNC24_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY9160017.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPHDHPLANNPNVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYHL
                     TPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWP
                     KHHEQSPASRNFRIHLLNALR"
     gene            2738..3958
                     /gene="mlc"
                     /locus_tag="JNC24_003342"
                     /gene_synonym="dgsA"
     CDS             2738..3958
                     /gene="mlc"
                     /locus_tag="JNC24_003342"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor Mlc"
                     /protein_id="HAY9160018.1"
                     /translation="MVAENQLGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVQELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDLPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGAGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIITGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            4083..4778
                     /gene="bioD"
                     /locus_tag="JNC24_003343"
     CDS             4083..4778
                     /gene="bioD"
                     /locus_tag="JNC24_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HAY9160019.1"
                     /translation="MLERFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEVIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(4731..6023)
                     /gene="clcB"
                     /locus_tag="JNC24_003344"
     CDS             complement(4731..6023)
                     /gene="clcB"
                     /locus_tag="JNC24_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001315626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HAY9160020.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAAFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSPWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLVSNLINHSDAL
                     LYSVQLSVTVQASDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(6182..6796)
                     /gene="dmsD"
                     /locus_tag="JNC24_003345"
     CDS             complement(6182..6796)
                     /gene="dmsD"
                     /locus_tag="JNC24_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HAY9160021.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            complement(6839..7693)
                     /locus_tag="JNC24_003346"
     CDS             complement(6839..7693)
                     /locus_tag="JNC24_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HAY9160022.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQRIV
                     RGMFFLWLLMGIGFMASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAGVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLVLGLILILVGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            complement(7695..8312)
                     /gene="dmsB"
                     /locus_tag="JNC24_003347"
     CDS             complement(7695..8312)
                     /gene="dmsB"
                     /locus_tag="JNC24_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HAY9160023.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCDEPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTSDTTGYLANPEEV"
     gene            complement(8323..>9397)
                     /locus_tag="JNC24_003348"
     CDS             complement(8323..>9397)
                     /locus_tag="JNC24_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HAY9160024.1"
                     /translation="RDGVRGKEKLDVPIKFLWCYASNTLINQHGDINHTHEVLQDDSK
                     CEMIVGIDHFMTASAKYCDILLPDLMPTEQEDLISHESAGNMGYVILAQPATSAKFER
                     KPIYWMLSEVAKRLGPDVYQTFTEGRSQHEWIKYLHAKTKERNPEMPDYEEMKTTGIF
                     KKKCPEEHYVAFRAFREDPQANPLKTPSGKIEIYSERLAKIADTWELKKDEVIHPLPA
                     YTPGFDGWDDPLRKTYPLQLTGFHYKARTHSSYGNIDVLQQACPQEVWINPIDAQARG
                     IRHGDTVRVFNNNGEMLIAAKVTPRILPGVTAIGQGAWLKADMFGDRVDHGGSINILT
                     SHRPSPLAKGNPSHSNLVQIEKV"
BASE COUNT         2449 a         2379 c         2437 g         2132 t
ORIGIN      
        1 cagacttccg tgagtggttg gtttcagcgt gtaacgatat gtacaaacgc tgaataaatt
       61 acagcattga taataacgta ttgtgactta agggaaattt agctaccaat aatagtagtc
      121 ttgatcgggt taactatttt actacttaca aagcggtaga ataactgcgc atcagtaata
      181 aatgacacac agcaaaatga atccgtttat ttgggtactt ataatcctga tgacgctaga
      241 cgcgctgcgg gaattggctg gcgcttcgtc tattttagga tggctattaa cgttggtttg
      301 agcaagcaat aagtccggac gtgtagttac cgcagtccgg gcttattttt caggcgtgca
      361 gacgacgatg caaacgcgtc ccgaccagca gagcaatgac cagcatcaga gcaataaatg
      421 cgccgacgcc gttccagcca tagttatgcc agaaaacacc acccagcgtc ccggcaatac
      481 tcgaccccag atagtaactg aacagataca gcgaggaggc ctggccttta gcgcgttttg
      541 cgcgggggcc aatccagctg ctggcaactg agtgggctgc gaagaatcct gctgagaaga
      601 gtaacattcc ggcaaagatc agccacagcg agctgaataa ggtcatcagt aaaccaaaca
      661 gcataacccc cgtcgaaaac aacatcaccg gaccgcgccc gtagcgggtg gtcatggttc
      721 cggctttggg tgagctccat gtaccggtca aataagccag cgataataag ccaaccacgg
      781 cctgactgac atgccagggg gaaagcatca accgatagcc gatgtaatta aacagcgtga
      841 cgaacgaccc catcagcaaa aagccttctg cgaacaataa cggtaatccc cggtcacgcc
      901 agtgcagacg aaagttgata aataacgtct tagggcgcag cgaagtcggg cgaaaatggc
      961 gtgattccgg aaggattttc cagaacatca acgccgaggc tagcgcgaaa caaccgattg
     1021 ccgccagagc aattcgccag ttgaaaaagt ccgtgaagac accactaatt aagcgtccgc
     1081 tcatgccgcc aattgagttg ccgctgatat acaaccccat cgaaaaggcc acgaaactgg
     1141 ggtggatttc ctcgctaaga taagtcatgc caacagctgc cacgccactt aacgaaagcc
     1201 caatcaaggc gcgcataatc aaaatgccgt gccagctggt catcattgtc gaaagtaacg
     1261 tacaaatgga ggccaacagt agcgccgtga ccatcactgg tttgcgacca atggcatcgg
     1321 atagggggcc agtaaacagc aaaccaatag ccaacatcgc cgtggaaatg gacagtgaaa
     1381 tactactgtt cgcgggggtt aagccaaact cctgcgaaag caccggaagg ataggctgca
     1441 cacaatagag gagtgcaaag gttgccagtc cggcagagaa cagcgccagg gtgacgcgca
     1501 taaattgcgg cgtaccgcgt ttaataaatt gatttggctg agaaatgctt tgcttgtcag
     1561 tgtcgcttgc cggagcgcca tcaacagttg tagtacggct cacttgaaat ccttgctaaa
     1621 tatgcctgtg gatcaggctt atacataggt taggaaaatc gaattgttct gtctaatata
     1681 ttaataatct caaataagat gttttaaata tgaatattga acttcgtcat ctgcgttact
     1741 ttgttgctgt tgcggaagag ctgcatttcg ggcgcgccgc tgcccgcctg aatatttcgc
     1801 aaccaccgct aagtcagcag attcaggcac tggagcaaca aattggtgcc cgactgctgg
     1861 cacgaactaa tcgcagtgta ttgctgacgg cagcaggaaa acagtttctt gcagatagtc
     1921 ggcaaatcct gtctatggta gatgacgctg ccgctcgcgc tgaaagactg catcagggtg
     1981 aagcggggga gttgcgcatt ggttttactt cgtccgctcc ttttattcgg gcggtgtccg
     2041 atacgttatc gctgtttcgc cgtgattatc ccgatgtcca tttacaaacc cgcgaaatga
     2101 acactcgtga gcaaatcgct ccgctcattg aaggaacgct ggatatggga ttgctgcgta
     2161 acacagcgtt accggagacg cttgaacacg cagtcatcgt ccatgaaccg cttatggcga
     2221 tgatcccgca cgatcatccc ctggcaaata acccgaatgt aacgctggct gaactggcga
     2281 aagaaccctt cgtctttttt gatccgcacg tcgggacagg gctgtatgac gatattctcg
     2341 ggctgatgcg acgttaccat ttgacgcccg tcatcactca ggaggtgggc gaggcaatga
     2401 ccatcatcgg tctggtttcc gccggtctgg gtgtttcaat tttgcctgct tcatttaaac
     2461 gtgttcagct caacgaaatg cgctgggtgc cgattgctga agaggatgcg gtttctgaaa
     2521 tgtggttggt ctggccgaaa catcatgaac aaagtccagc ttcgcgtaac tttcgtattc
     2581 atctgctgaa tgctctcagg tgagggaaat ttcagcgaaa aagcccgaaa aatgtgctgt
     2641 taatcacatg cctaagtaaa aatttgacga cacgtattga agtgcttcac catagcctac
     2701 agattatttc ggagcgcgaa aatataggga gtatgcggtg gttgctgaaa accagcttgg
     2761 gcacattgat caaataaagc agaccaacgc gggcgcggtt tatcgcctga ttgatcagct
     2821 tggtccagtc tcgcgtatcg atctttcccg tctggcgcag ctggctcctg ccagtatcac
     2881 taaaattgtc cgtgagatgc tcgaagcaca cctggtgcaa gagctggaaa tcaaagaagc
     2941 ggggaaccgt ggccgtccgg cggtggggct ggtggttgaa actgaagcct ggcactatct
     3001 ttctctgcgc attagtcgcg gggagatttt ccttgctctg cgcgatctga gcagcaaact
     3061 ggtggtggaa gagtcgcagg aactggcgtt aaaagatgac ttgccattgc tggatcgtat
     3121 catttcccat atcgatcagt tttttatccg ccaccagaaa aaacttgagc gtctaacttc
     3181 gattgccata accctgccgg gaattattga tacggaaaat ggtattgtac atcgcatgcc
     3241 gttctacgag gatgtaaaag agatgccgct cggcgaggcg ctggagcagc ataccggcgt
     3301 accggtttat attcagcatg atatcagcgc atggacgatg gcagaggcct tgtttggtgc
     3361 ctcacgcggg gcgcgcgatg tgattcaggt ggttatcgat cacaacgtgg gggcgggcgt
     3421 cattaccgat ggtcatctgc tacacgcagg cagcagtagt ctcgtggaaa taggccacac
     3481 acaggtcgac ccatatggta aacgctgtta ttgcgggaac cacggctgcc tcgaaaccat
     3541 cgccagtgtg gacagtattc ttgagctggc acagctgcgt ctcaatcaat ccatgagctc
     3601 gatgttacat gggcaaccgt taaccgtgga ctcattgtgt caggcggcat tgcgcggcga
     3661 tctactggca aaagacatca ttaccggggt gggcgcgcat gtcgggcgca ttcttgccat
     3721 catggtgaat ttatttaacc cacaaaaaat actgattggc tcaccgttaa gtaaagcggc
     3781 agatatcctc ttccctgtca tctcggacag catacgtcag caggcccttc ctgcgtatag
     3841 tcagcacatt agcgttgaga gtactcagtt ttctaaccag ggcacgatgg caggcgctgc
     3901 gctggtaaaa gacgcgatgt ataacggttc tttgttgatt cgtctgttgc agggttaaca
     3961 ttttttaact gttctaccaa aatttgcgct atctcaattt gggccaggaa agcataactt
     4021 agactttcaa ggttaattat tttcctagtt tatatttgtg aagcataacg gtggagttag
     4081 tgatgctgga gcgtttcttt attaccggta cagacacttc tgtagggaaa acggtggttt
     4141 cccgcgcatt gctacaagcg ttagcctccc agggaaaaac agttgcggga tataaacccg
     4201 tagcgaaggg gagcaaagag acacccgaag ggctgcgtaa taaagatgcc ctggtgttgc
     4261 agagtgtttc aaccatcgaa ctgccttatg aagcagttaa tcctatcgcg ttaagcgaag
     4321 aagaaagtag cgtggcgcac agttgcccaa tcaattacac cctcatttca aacggcctgg
     4381 caaacctgac cgataaagtc gatcatgtcg tggtagaagg gactggcggc tggcgcagtc
     4441 tgatgaatga tttgcgtcca ctctctgaat gggtggtgca ggaacagttg ccggtgttga
     4501 tggttgtcgg tattcaggaa ggttgcatta accatgcact gctaacagct caggcgattg
     4561 ccaacgacgg gctgccgctc attggctggg tggctaaccg aatcaaccca ggactggcgc
     4621 attatgcgga agtcattgat gtgctgggta aaaaacttcc ggcaccgctc attggtgaac
     4681 tgccttatct gccgcgcgcc gaacagcgag aactggggca atacatccgc ttagctatgc
     4741 tgcgcagtgt gctggcggta gatagagtca cggtgtaacg tccgcgaaat taccgacgca
     4801 atgacgcagg caatcaataa accggggagt agctgatact ccccggtcat ttcacatatc
     4861 atcaacgtcg acataatcgg cgcgtgcgtg gttgccgcca acagtgtcgc catcccggtc
     4921 aatccgagta aaagcgtaat ttcttcgcca tcagggaacc ataatcccag gctacgacca
     4981 tacaacatgc caatggcaag accgataaat agcgtcggtg taaagacacc accgggtgcg
     5041 ccggaaccgc tactcgccag cacggcacac agtttacaaa ggaagatccc ggcaatgatc
     5101 attaacagtg gtggggcggt taaaaaggat tgtacggtgc tatagccgtt gccccacact
     5161 gcaggtgtga acagggaaag cagacccacg atcaacccgc ctagtgccag ttgccagggc
     5221 ggcgcaagtt tgagactcac aaatccacga tgacaggcgt tcattaacgt taacaacagt
     5281 ggtccgcaca gacctgccag cacacctgta ctgataatca gcgcatagtc actagcctga
     5341 accgttactg agagttgtac gctgtagagt aacgcgtcgc tatgattaat aagattgcta
     5401 accagcaacg ccacgacggc ggaaataatc accgggccga gagaggccaa catcatagtg
     5461 ccaaacagca cttcggctat aaataaactg ccagcaagcg gggcgcgata ggccgcagcc
     5521 attcccgccg cggccccaca ggcgatccat aatttccatt cctggcgtgg cgtaaaacgt
     5581 tgggcaaaac aggaggcggc aagggcagct aaaagaatca tcgcaccttc gcgaccaatt
     5641 gcactgccgc tggttactac cagcagcgag gcaagcgatt taaccaggct tgctgcgtaa
     5701 tcgaactgtc catcggtttg caacgcttcc atgtaatcgg tcggcgcatg agggcgttgt
     5761 tgggtaaatt tctgccagcc catcagcaac aaacccgccg ccagtccgcc gagcgccgga
     5821 gttagcagcc gtcgccaggg ggaaaggttt gtcgctgcat tgaccagact gccggagtca
     5881 ttattgagga acaaccactc cagtagcagc atcgcatgac gaaacccggc aacggcaaag
     5941 gccgcgagaa taccgacgac tgttgcgata agcagacggc ggaacatagt gcgcagatcc
     6001 ggatagatgt gtagatgatg catgagcaga aatgggcaat gaaacacggg cgactatttt
     6061 gcggcaaaag aggccaaccg caaaagcagt gcgtgcttta agagataaaa aaacgtgaca
     6121 ttgtaactta ttattgccga atcgcgcgta atcgtcacca tccggcaata ttacggtgat
     6181 cctatcgaaa gagtggttta accgcgacag gaatcaacag ttgtgattgc cattgcgcca
     6241 gcgttaagcg agccagttca cccagtgcac ggtagaaagg gtgttctgct ttttcgataa
     6301 aaacatcaag aaaacgtgtt gaccacggaa aaaggtgcca tgccagcaat tcttcgcatt
     6361 ctgtctggcg accattctcc gccaaccacg cagccatcaa cagcagtgaa ccaaaatgat
     6421 cttccggttc gttttgcttc atttcaaact gaatgccttt ctcgcgcatc cactgacgaa
     6481 gtgccaatgt tgaatcgcca aacagcacag attcgcgatc cagccagacc gatccccacg
     6541 gcggagacgg cagtgcccac gggccgacaa acaaacgctg ccaggcctgg gcgtgagtct
     6601 cttcacactg tgtctgaaaa gccgtcacca gcggcgctaa tgacgcttct ggtagaggcc
     6661 actgagtttc ccagccgtca ctggtgagta ccgcaaccag aggtgcggct tccgcactct
     6721 ctggagcgta ataaaacagc gcgcccaaca cgcgcgccgc gacagaaaaa ttatcttgct
     6781 gtgaaaaatg ggtcattcct aacatcctga aatgtgcggg ttgccccgca ccaatttgtt
     6841 aacctgcaat agccattcct actgtcatgt gcaggccata aaagagcaca cgcccgatca
     6901 tctcaccaac aagtatgagg attagcccca gaacaagtcc ggcaacgtga ggttcacggc
     6961 gacgaatgag cggacaaagc cagcatccaa gacctgcgca taacaatacc acacgccaga
     7021 cctgtaagga ggcgtaatct ggtaccagtg cgctggcttg ttgcacggat gagtgaatag
     7081 acgccagaga caaaccttgc agcacaatca ccccggcaca agcgatcagc gcgagaacgc
     7141 taatgatggc aaatggtgtg gtattaaatg taacgcgggc tgcccgcaga attgcggcag
     7201 caagtattgg accgctcaac aataccgtca ggaagaaagc cagcgtagtg taaccgttat
     7261 gccaggttgg cacagtgtcg atttgataca cgcaggtcat catccagacg aaaatgacgc
     7321 cgagcgccat gctgacaagt aaccagagtt tccccaacgc ttgcggcatt ttaccgatga
     7381 ccgccaccag ccaccatagt ccgccaacgg caaaaaatat ggaaccggca gcaatctcgt
     7441 tactcagacc agaagctccg attcgattca gcgagttaaa cgcccgcagt ggcgatccca
     7501 ggtgcatgac agaggccata aacccgatgc ccatcaacag ccagaggaaa aacatgccac
     7561 ggacaatacg ctgacggtct gcgtcatttt tggcggcgaa ccagccaatt ccgctaacaa
     7621 tcagcgcgcc tactacgcat tgacccaata cggtaaagat aaccagtggc cactcatgcc
     7681 atccatttcc cattttacac ttcctccgga ttagccagat aaccagtggt atcgctggtc
     7741 gggcggctgt tggcgttagg tttgataacg atattgggtt ttgtgaaatg cgcgcgcggc
     7801 agtggcgcga cggcagccag agtgccgtgc ttctggcgca gctcttcaat tggaccgaac
     7861 tcaagtgcac gcagcgggca ggattcgaca catatgggtt gtttcccctc ggcgacgcgc
     7921 gaataacaac catcgcactt cgtcatgtgc cctttttcag cattgtactg tggcgcgccg
     7981 tacggacaag ccatgtggca gtagcggcag ccgatacaaa catcttcatc aaccaccaca
     8041 aagccatctt cacgcttatg cattgcgccg ctggggcaga cttttgtaca tgcgggttca
     8101 tcgcaatggt tacaggagat ggagagataa taagcgaaca cgttctggtg ccagacgcca
     8161 ttatcttcct gccagtcgcc gccagcatat tcataaatac ggcggaaact gacttccggg
     8221 ccaagatctt taaaatcttt gcacgccagt tcgcaggttt tacagccagt acagcggctg
     8281 gaatcgataa aaaatccata ttgtgtggtc acgggctact ccttaaacct tttcgatctg
     8341 gacaagattg ctgtgcgacg ggtttccctt tgccagcggt gaagggcggt gagaggtcag
     8401 aatattgata ctgccgccgt gatcgacccg gtcaccaaac atatccgctt taagccaagc
     8461 accttgcccg atggcggtaa cgccaggcag aatacgcgga gtcacttttg cggcaatcag
     8521 catttctcca ttattgttaa atacccgcac ggtatcgcca tgacggatac cgcgtgcctg
     8581 agcatcaatg gggttgatcc acacctcttg tgggcaggcc tgctgtaaca catcaatatt
     8641 gccgtagctg gagtgggtac gcgctttgta atggaagccc gttaactgca gtggataggt
     8701 tttccgcagg ggatcgtccc agccatcaaa acctggggta tacgcaggaa ggggatgaat
     8761 aacttcatct tttttcaatt cccaggtatc cgcaatcttc gccagtcgtt cagaataaat
     8821 ttcgattttc cccgaaggtg ttttcaacgg gtttgcctgt ggatcttcac ggaatgcgcg
     8881 gaaagcgacg tagtgttctt ccgggcattt tttcttaaag atcccggtcg ttttcatctc
     8941 ctcgtagtcg ggcatctcag ggttacgttc cttcgttttc gcatggagat atttgatcca
     9001 ttcatgctga ctgcgacctt cagtaaaggt ttgataaacg tctggtccta agcgtttggc
     9061 gacttcactc agcatccagt agatgggttt gcgttcaaat tttgctgagg ttgcgggttg
     9121 ggcgaggatc acatagccca tattccctgc agattcatga gagataaggt cttcttgctc
     9181 tgttggcatc aggtcgggca acaggatatc gcaatactta gccgaggccg tcatgaagtg
     9241 gtcaatgcca acaatcatct cgcacttgct gtcatcctga agcacctcat gggtgtgatt
     9301 gatgtcgcca tgttgattga tcaatgtgtt actggcgtag caccataaaa acttgatggg
     9361 gacatccagt ttttcttttc cacgaacacc atcacgg
//
LOCUS       DADMKF010000151         9213 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.154, whole genome shotgun sequence.
ACCESSION   DADMKF010000151 DADMKF010000000
VERSION     DADMKF010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9213
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.154"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            74..733
                     /locus_tag="JNC24_003349"
     CDS             74..733
                     /locus_tag="JNC24_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HAY9160025.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSL
                     VGAGIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     GDNIILNMPNNVTFDSSSATLKPAGANTLTGVAMVLTEYPKTAVNVIGYTDSTGGHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSP
                     L"
     gene            837..1811
                     /gene="ghrB"
                     /locus_tag="JNC24_003350"
     CDS             837..1811
                     /gene="ghrB"
                     /locus_tag="JNC24_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HAY9160026.1"
                     /translation="MKPSVILYKALPDDLLQRLQEHFTVHQVANLSPQTVEQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLHATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(1861..2571)
                     /locus_tag="JNC24_003351"
     CDS             complement(1861..2571)
                     /locus_tag="JNC24_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HAY9160027.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            3005..3295
                     /locus_tag="JNC24_003352"
     CDS             3005..3295
                     /locus_tag="JNC24_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HAY9160028.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIEQLRKG
                     TGLKIDDFARVLGVSVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLME"
     gene            3576..3788
                     /gene="cspA"
                     /locus_tag="JNC24_003353"
     CDS             3576..3788
                     /gene="cspA"
                     /locus_tag="JNC24_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HAY9160029.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(3976..4128)
                     /gene="hokA"
                     /locus_tag="JNC24_003354"
     CDS             complement(3976..4128)
                     /gene="hokA"
                     /locus_tag="JNC24_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HAY9160030.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQESYELAAFL
                     ACNLKE"
     gene            complement(4454..6523)
                     /gene="glyS"
                     /locus_tag="JNC24_003355"
     CDS             complement(4454..6523)
                     /gene="glyS"
                     /locus_tag="JNC24_003355"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HAY9160031.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLDAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRLNRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            complement(6533..7444)
                     /gene="glyQ"
                     /locus_tag="JNC24_003356"
     CDS             complement(6533..7444)
                     /gene="glyQ"
                     /locus_tag="JNC24_003356"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HAY9160032.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(7539..7838)
                     /locus_tag="JNC24_003357"
     CDS             complement(7539..7838)
                     /locus_tag="JNC24_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9160033.1"
                     /translation="MMMNAFFPAMALMVLVGWSTPSPVQKAQRVKVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            8013..9008
                     /locus_tag="JNC24_003358"
     CDS             8013..9008
                     /locus_tag="JNC24_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HAY9160034.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLIFYSAIALLYIALFTSINM
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMAVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(9050..>9213)
                     /locus_tag="JNC24_003359"
     CDS             complement(9050..>9213)
                     /locus_tag="JNC24_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9160035.1"
                     /translation="GLWNATLLLSEKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKET
                     QVTQEEYSE"
BASE COUNT         2286 a         2327 c         2268 g         2332 t
ORIGIN      
        1 ttcatgttca cgcggcataa tctcccgcca cggaacccgt ggcaaagaat aaaaaggtta
       61 ttaaggatta acaatgaaga aacgtgttta tcttattgcc gccgtagtga gtggcgctct
      121 ggcggtatct ggctgcacaa ctaaccctta caccggcgaa cgcgaagcag gcaaatctgc
      181 tatcggcgca ggtctgggct ctctcgtggg cgcgggtatt ggtgcgctct cttcttcgaa
      241 gaaagatcgc ggtaaaggcg cgctgattgg cgcagcagca ggcgcagctc tgggcggcgg
      301 cgtgggttat tacatggatg tgcaggaagc gaagttgcgt gacaaaatgc gcggcactgg
      361 tgttagcgta acccgcagcg gggataacat tatcctcaat atgccgaaca atgtgacctt
      421 cgacagcagc agcgcgaccc tgaaaccggc aggcgctaac accctgaccg gcgtggcaat
      481 ggtgctgaca gagtatccga aaacggcggt taacgtgatt ggttataccg acagcacggg
      541 gggtcacgac ctgaacatgc gtctctccca gcagcgtgcg gattccgttg ccagcgcgtt
      601 gatcacccag ggcgtggacg ccagccgcat ccgtactcag ggccttggcc cggctaaccc
      661 aatcgccagc aacagcaccg cagaaggtaa ggcgcaaaac cgccgtgtag aaattacctt
      721 aagcccgctg taatcccttt catgccaggt gatgcagaaa tcacctggca ttcttcagtt
      781 ttccttcatc atatttcagg ctaaggtgat cgccttatca gtgaatggag agaagcatga
      841 agccgtccgt tatcctctac aaagccttac ctgatgattt actgcaacgc ctgcaagagc
      901 atttcaccgt tcaccaggtg gcaaacctca gcccacaaac cgtcgaacaa aatgcagcaa
      961 tttttgccga agctgaaggt ttactgggtt caaacgagaa tgttgatgcc gcattgctgg
     1021 aaaaaatgcc gaaactgcat gccacatcaa cgatctccgt cggctatgac aattttgatg
     1081 tcgatgcgct taccgcccga aaaattctgc tgatgcacac gccaaccgta ttaacagaaa
     1141 ccgtcgccga tacgctgatg gcgctggtgt tgtctaccgc tcgtcgggtt gtggaggtag
     1201 cagaacgggt aaaagcaggc gaatggaccg cgagcatagg cccggactgg tacggcactg
     1261 acgttcacca taaaacactg ggcattgtcg ggatgggacg gatcggtatg gcgctggcac
     1321 aacgtgcgca ctttggcttc aacatgccca tcctctataa cgcgcgccgc caccataaag
     1381 aagcagaaga acgcttcaac gcccgctact gcgatttgga tacactgtta caagagtcag
     1441 atttcgtttg cctgatcctg ccgttaactg atgagacgca tcatctgttt ggcgcagaac
     1501 aattcgccaa aatgaaatcc tccgccattt tcattaatgc cggacgtggc ccggtggttg
     1561 acgaaaatgc actgatcgca gcattacaga aaggggaaat tcacgccgcc gggcttgatg
     1621 tcttcgaaca agagccactg tccgtagatt cgccgttgct ctcaatggcc aacgtcgtcg
     1681 cagtaccgca tattggatct gccacccatg agacgcgtta tggcatggcc gcctgtgccg
     1741 tggataattt aattgatgcg ttacaaggaa aggttgagaa gaactgtgtg aatcctcacg
     1801 tcgcggacta agccgcgact gcgtgaagta aagcccgata atcgctcggg cttttactct
     1861 ttattgggtt gcagtaactg ctgtagtcca ggcctgatta aacgcctgat gttgtgccgg
     1921 taatggcgca atcagtttgt tatattcact tgcctgctgt gaagtcggga actggatgcc
     1981 atttgcgaca aagctcacct gagtaccttg ctgagcaatg tagtctccaa ccatcaccag
     2041 ttgttgcgtg aaagtttgtg ccgccggaat caacggttgc aatgcatcag ctggcgtcgt
     2101 taccactttg gtgaatgcct gatcaaatac cggtttcagg tcatcgctct gttttagcgc
     2161 agagtgcgca gcatcggcct gcaacttcgc attttgcagt tgttgcgcca gcacacccag
     2221 cgaaccattc atttcacgca gcggaccgct ttgcgtaacg taatcctgcg gtacgcgaat
     2281 tgcgttaacg ctgtctacga ccgggcgcag accagaatcc atcgcctgat tcacctgctg
     2341 agaataacca tacagaatcg cgtaatcaga gacaaaagga ccaaactgtt ttttctgatc
     2401 ggcagtcagg gttggtagac gttcaccgct acgcatcact gtattctgca ggaagtcgat
     2461 aaacgcttta cgctgatcgc cttctttatc aaaacaccca ctcaggctaa ctaccattaa
     2521 taacgccgca agaggcgcaa accagcgaga gcaggacttt cctgtcgcca ttttcttact
     2581 cctttcaccc aaaaaaagcg cacaacgaca cacgcgtgtc tgacgtgaca aggatagtcc
     2641 aggacaggct tgcaggatac cctttctacg taatcttcct ggtaaaatca tggagaaaat
     2701 gaaacaaggt taatttgttg tttcattgtt aaaaaatgat agtgccgttg ccttttttgt
     2761 agccagagat ttacgccttc gcagaaggtt agattttcga aatctttaca aaacaggagt
     2821 ggtaagagat atatcggcat ttatcagatc gttaggtggc tataagtcac gctaaatgac
     2881 aggctgaatc gaatcatagc cagagcatgc cctgacttca ccccgctgtg tctgcttttc
     2941 ccgactattc ttaatgagct tcgatgcaat tcacgatccc gcagtgtgat ttgaggagtt
     3001 ttcaatggaa tataaagatc caatgcatga gctgttgagc agcctggaac agattgtttt
     3061 taaagatgaa acgcagaaaa ttaccctgac gcacagaaca acgtcctgta ccgaaattga
     3121 gcagttacga aaagggacag ggttaaaaat cgatgatttc gcccgggttt tgggcgtatc
     3181 agtcgccatg gtaaaggaat gggaatccag acgcgtgaag ccttcaagtg ccgaactaaa
     3241 attgatgcgt ttgattcaag ccaacccggc attaagtaag cagttgatgg aatagacttt
     3301 tatccacttt attgctgttt acggtcctga tgacaggacc gttttccaac cgattaatca
     3361 taaatatgaa aaataattgt tgcatcaccc gccaatgcgt ggcttaatgc acatcaacgg
     3421 tttgacgtac agaccattaa agcagtgtag taaggcaagt cccttcaaga gttatcgttg
     3481 atacccctcg tagtgcacat tcctttaacg cttcaaaatc tgtaaagcac gccatatcgc
     3541 cgaaaggcac acttaattat taaaggtaat acactatgtc cggtaaaatg actggtatcg
     3601 taaaatggtt caacgctgac aaaggcttcg gcttcatcac tcctgacgat ggctctaaag
     3661 atgtgttcgt acacttctct gctatccaga acgatggtta caaatctctg gacgaaggtc
     3721 agaaagtgtc cttcaccatc gaaagcggcg ctaaaggccc ggcagctggt aacgtaacca
     3781 gcctgtaatc tctgcttaaa agcacagaat ctaagatccc tgccatttgg cggggatttt
     3841 tttatttgtt tttcaggaaa taaataatcg atcgcgtaat aaaatttagt attatttttg
     3901 tgaagaataa atttgggtgc aatgagaatg cgcaacgccg taagaaaggc gggaataact
     3961 tcccgccaaa gactcttact ctttcaaatt gcaggctaaa aatgccgcca gctcataact
     4021 ctcctgttta atatgcaatt cacacagtga atctcttatc atccaggtga aaaataaaag
     4081 cgtgaaacaa atcactatta aagaaagtaa tctatatttc tgcggcattc ccagcctcct
     4141 gtgttgattt ccaacgagta agaggctaac cttatggtgt tcaggcatag ggtagcctca
     4201 ctttgattta tagtcaggtg gggcttttct ctgtctgcct ttcggtgaat acctgagaca
     4261 aacagtctca agcacccgtg gctattctag cttaataagt ttgtttcttc tccttgatat
     4321 aatcctaaaa aaacctcata aaattaatat atgagataat ctttattcaa cagaagatta
     4381 ttaaaggttg ctgtattatt tagcgataat aaaaaaagcc tgccagatgg caggcttttt
     4441 aataacggcg ttattactgc aacagcgaaa tatccgcaac gcgcaggaac agttcgcgca
     4501 gtttctccag catggtcaga cggttgagac gcaattcttt gtcatcaacc atgaccatca
     4561 ctttatcgaa gaaagcatca accggctcac gcagctctgc cagttcgacc agcgcatcct
     4621 ggtaacgacc ttccgcaaag tacggctcca gcttgtcacg tagcaccaca acctgcatcg
     4681 ccagtttaat ttcttccggc tctttcaggg tagaggcatt cacgcggtcg ctcagcactt
     4741 cgtcagattt cgccagaatg ttagatacac gtttgttcgc cgccgccagt gcagcagctg
     4801 catccagggt acggaaatgc gataccgctt tcatacgggc atcgaaatca gccggacgag
     4861 tcggacgacg cgccagtacc gcctggatgg tgtcaacggt gtaaccttcg tcctgatacc
     4921 aggcgcggaa gcgaccgagc ataaagtcga taacatcatc aactacgttg gcgttagtca
     4981 gcttatcgcc atacagacgc actgcttctt cggtcagcgt ttgcagatca aggttgaggt
     5041 tcttctcaac gataattcgc agcacgccaa gtgcggcacg acgcagcgca aacgggtctt
     5101 tgtcgccttt cggatgctga ccgataccga agatacccgc cagagtatcc atcttatcag
     5161 caatcgccag cgcacaagct accgggttgg acggcaggtc atcaccggca aagcgcggct
     5221 gatactgctc attcagcgcg acggctacgt cttccgcttc gccatcgtga cgcgcgtagt
     5281 gcatccccat aacgccttgg gtgtcggtga actcgaagac catgttggtc atcaggtcac
     5341 acttagacag caggcccgca cgggttgcgt ggttaacgtc agcgccaatc tgttcagcaa
     5401 tccagccagc cagcgcctgg atgcggtcag ttttgtcacg cagcgtaccc agctgttgct
     5461 ggaacagcac ggtttgcagg cgcggcaggt tatcttccag acgtttttta cggtcggtat
     5521 tgaagaagaa ctcggcatcc gccagacgtg gacgaacgac tttctcgtta ccagagataa
     5581 tttgctgcgg atctttcgat tcgatgttgg caacgaagat gaagttcggc agcagtttgc
     5641 catcgttcgc atacaccggg aagtatttct ggtcaccttt catggtgtaa accagggctt
     5701 cagccggcac cgcgaggaat ttctcttcga attttgcggt cagaacgacc ggccactcca
     5761 ccagcgaagc cacttcttcc agcaggcttt cgcttaagtc agcgttaccg ccaatcttac
     5821 gcgctgcttc ttcggcatcg gctttaatct tcgctttacg ttcttcgtaa tcggcgatga
     5881 ctttcccgcg ctcacgcaga atttccggat actgatcggc gttgtcgatg gtgaattccg
     5941 gttcgcccat aaagcggtgg ccgcgaatca cgcgatcgga ctgaatgccc agaatggttg
     6001 ccggaatgac tttgtcgccc agcagcaggg tcacggtgtg caccggacgc acgaagtgca
     6061 cgtcgcttgc gccccaacgc atcagtttcg ggatcggcag tttcgccagc gaagtcgcaa
     6121 ccatattcgg cagtagtgct tcagtgcttt cgcccttcac atgggcgcga tacagcagcc
     6181 attcgccttt atcggtagtc agacgctcag cctggtcaac ggtaataccg caaccacgcg
     6241 cccaaccttc tgccgctttg ctcggtttgc cttcagcgtc gaacgcctgg gcaatcgccg
     6301 ggccgcgttt ttcgatttca cgatctggtt gcgcttccgc caggttagct actttcaacg
     6361 ccagacgacg cggagcagca aaccattgaa cggtgccgtg tgcgaggcca gcgttatcca
     6421 gctccgcagt aaagttcgca gcaaaggact cagccaggct gcgcagtgct tttggtggca
     6481 gctcttcagt gccgatttcc accagaaaag ttttctcaga catagccgcc tcttacttat
     6541 ctttgttgca catcgggaag ccgagggctt cacgggaagc gtagtatgct tctgccactg
     6601 ctttggtcag ggtgcgaatg cgcagaatgt agcgctgacg ctcagtgacg gagatggctt
     6661 tacgcgcatc cagcaggttg aagctgtggg cggctttcag aatacgctcg taggctggca
     6721 gcggcagcgg attttccagc gccagcagct gctgcgcttc tttctcgtac tgctcgaagc
     6781 aggtgaacag gaaatccaca tccgcgtatt cgaagttgta agtggactgc tccacttcgt
     6841 tctgatggaa cacgtcgccg taggtggttt tacccagcgg gccgtcgctc cagaccaggt
     6901 cgtaaacgct gtctacgccc tgaatgtaca tggccagacg ttccagaccg taggtgatct
     6961 cgccggtaac cggtttacac tccagaccac caacctgctg gaagtaagtg aactgcgtca
     7021 cttccatgcc gtttagccac acttcccagc ccagtcccca ggcacccaga gtcgggtttt
     7081 cccagttatc ttccacgaaa cggatgtcgt gaatagtcgg gtccatgccc agctctttca
     7141 gagaaccgag gtacagctcc tgaatattgt ccggcgatgg cttaatgacc acctggaact
     7201 gatagtagtg ctgtaaacgg ttggggtttt cgccgtagcg accatcggtc gggcgacgag
     7261 aaggctgaac ataagcagcc gccatcggtt ctggccccag cgcgcgcaga caggtcattg
     7321 ggtgagaggt tcccgcgccg acttccatgt ccaatggttg aacaatggtg cagccctggc
     7381 gagcccagta atcctgtaag gtcaggatca agccctggaa ggtcctggta tcaaactttt
     7441 gcatattatt tcgtgctgga tacgtgtgga tttaaaggaa gggatcagta tacccgctgg
     7501 atggaagata tacagtacga aacgggaaaa agcagggctt aacgcatgga aagatgcaaa
     7561 aaatggccat ctgcgtcaaa agaacagaca aactgctctt tacggaaggt ataaccgcgc
     7621 atttcatagc ttccctggaa ctgttcgaag gcggtgacgt cgattttttg ctcgccggtg
     7681 ttataacgtt ttgccgcctg atccttgcat aacgcttcca tattcaacga acgcagagga
     7741 tcaaccttta cccgttgtgc tttctgcacg ggtgacggtg tagaccaacc cactagcacc
     7801 ataagcgcca ttgccggaaa gaaagcgttc atcatcatta aattaccgtc tggtttgcag
     7861 gtgagtctta ttatttatat cggtatagtg gctgatatct tacgaatttc cttaagcctc
     7921 tataagctgt agtgataaaa ctcagattat tccagtgcgc caaccaaatg atttatcggt
     7981 cagacttttt ttaaccagat acagagacac agatgcagcc caaaatttac tggattgata
     8041 acctgcgagg gatagcgtgt ttaatggtgg tgatgattca caccactacc tggtatgtga
     8101 ccaatgctca tagtgttagc cccgtcacat gggatatcgc caatgttctg aattctgcct
     8161 ctcgtgtcag cgtgccgcta tttttcatga tttccggcta tctctttttt ggcgaacgca
     8221 gcgcccagcc gcgccatttc ttgcgtatcg gcttatgtct gattttttat agcgcaatcg
     8281 cactgctcta cattgcgctg tttacctcca tcaatatgga gttagcgctg aaaaacctgc
     8341 tgcaaaagcc agtgttttac cacttgtggt ttttcttcgc gattgcggtg atttatctgg
     8401 tttcaccgct gattcaggtg aagaacgtcg gcggaaaaat gttgctggtg ctaatggcgg
     8461 tgattggcat tatcgctaac ccaaacacag tgccgcagaa aattgacggt tttgaatggc
     8521 tgccaattaa cttatatatc aatggcgata ctttttacta cattctgtat ggcatgttgg
     8581 gccgcgctat agggatgatg gacacacagc ataaagcact gtcgtgggtg agcgccgcgc
     8641 tgtttgcgac gggggttttt attatctctc gcgggacatt atatgaattg cagtggcgcg
     8701 gaaattttgc cgatacctgg tatctttact gtgggccgat ggtttttatc tgcgcaatcg
     8761 cgctattgac tctggttaaa aacacgctgg atacgcgtac cattcgcgga cttggcttaa
     8821 tctcccgcca ttcattgggt atatacggat tccacgcctt gattatccat gcgctgcgca
     8881 cccggggaat tgagcttaaa aattggccaa tactggatat tatttggatc ttttgcgcga
     8941 cgttggcagc gagtttgtta ctttctatgc tggtacaacg aatcgacaga aacagattag
     9001 tgagttaagt aaaagcccgt tcacattgga ctgaccgggc ttacgtgaat tattcgctgt
     9061 attcttcctg ggtaacctgt gtttcttttg gcgggtttat tccggcatca cgaatattct
     9121 tctgcaccgc tactgcacca aacaagctta agaagaaagc cagtccataa aaaccttttt
     9181 cactgagtaa taatgtcgca ttccacagac cta
//
LOCUS       DADMKF010000152         9188 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.155, whole genome shotgun sequence.
ACCESSION   DADMKF010000152 DADMKF010000000
VERSION     DADMKF010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9188
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.155"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            171..1769
                     /locus_tag="JNC24_003360"
                     /pseudo
     CDS             171..1769
                     /locus_tag="JNC24_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417880.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-dependent transcriptional regulator"
     gene            complement(1751..2509)
                     /locus_tag="JNC24_003361"
     CDS             complement(1751..2509)
                     /locus_tag="JNC24_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HAY9160036.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(2526..3356)
                     /gene="glpG"
                     /locus_tag="JNC24_003362"
     CDS             complement(2526..3356)
                     /gene="glpG"
                     /locus_tag="JNC24_003362"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HAY9160037.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWQAGHTGSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPALKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(3401..3727)
                     /gene="glpE"
                     /locus_tag="JNC24_003363"
     CDS             complement(3401..3727)
                     /gene="glpE"
                     /locus_tag="JNC24_003363"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HAY9160038.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            3917..5422
                     /gene="glpD"
                     /locus_tag="JNC24_003364"
     CDS             3917..5422
                     /gene="glpD"
                     /locus_tag="JNC24_003364"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HAY9160039.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDDRM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNTHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGAISDLGEDFGHEFYEAE
                     LKYLVDHEWVRRTDDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(5474..6079)
                     /locus_tag="JNC24_003365"
     CDS             complement(5474..6079)
                     /locus_tag="JNC24_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9160040.1"
                     /translation="MIKFRLYIPPVILGFVIVPLLVWPTVIVLAVLIFTLTFLAEIIF
                     SFPLLVVRISLQELQLELMVEYALFFSVMAGIGWQFSRRTPPELKNRLHCWLVFSPVY
                     FWLILSNFILYISPEKSALLENIRNFFLTFVWPPLNFSPFWPQPWTDFVGPISAQLGF
                     ALGYYCHWCRKNRNQRKKWGDWVTCLSLAILAQGPLFNYLQ"
     gene            complement(6082..6849)
                     /locus_tag="JNC24_003366"
     CDS             complement(6082..6849)
                     /locus_tag="JNC24_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9160041.1"
                     /translation="MQPSPAMQALIEQIYHTFRRYRVPQKFVVCCEYCLSQQEQKALR
                     STSLRAIPYSLINAWNSSPGPDPQNSDEVRYFLPRLLEFVAQGHFDNIHVVFSLRRIN
                     LANKENWRADERAILQRFACQYMTDWVSGDEAVELQYMLEMFFRADIALAPLLDAINS
                     VPGFWSTVSLACLLNRYCEDYIRDNQDDIDNAITTQINAWARNNHPLLKERARQAIEN
                     PLKQPEPMTEYQVWEDDWIIDECLCAMYDASSESPGK"
     gene            complement(6852..7562)
                     /locus_tag="JNC24_003367"
     CDS             complement(6852..7562)
                     /locus_tag="JNC24_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9160042.1"
                     /translation="MELVNTLFASLAGTDPFTGVDITIANCKSAYWDEGIVQQLINQA
                     LDEGEKFVGADGLEGLLRYDVTLNIGLTSSKVWPGFSLDTATISRLCACGADFGFDPY
                     ISDVPDVQCDLNTTNDVTVQFTAMLNPDERVIIAKRPLKKCDSWIEDVYIFQVFKDAW
                     KFHNNNSLRGFRDKQAELKLYTRHYSVENCAEESCRDCNSCIRPSFSLSRSALIRLNA
                     ANARFVYQPFTRDQRARG"
     gene            complement(7578..9083)
                     /locus_tag="JNC24_003368"
     CDS             complement(7578..9083)
                     /locus_tag="JNC24_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001472437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HAY9160043.1"
                     /translation="MQNRKWILSSLVMTFFGIPILTQFLAAVVAMLGVGLAGIIEVCN
                     ILITPTSYLLLNIFMLALGALMLFFSGRVWAGDSAPEKREIAVWRQCLFLVPGLLILV
                     GWIIALHLADYQFHQMGSGWLADLMLPWLGVLLVSVVGGEYWWIVIIPVGAHISFSLG
                     YGRPTRHPLTGTSGLRCRNSLLFILLMLGFVAGYQGYLYKQLNPGVGVRENIDTWAWR
                     PDKLNNQLTPLRGKPQIQFTQNWPRLDGATAAYPIYASAFYALSVIPEDFHTREYLES
                     SRTPDAYNRIVKGDADIIFVAQPSGGQKKRAEESGVTLLYTPFAREAFVFIVNADNPV
                     NSLTEQQVRDIFSGAITNWRTVGGNDQEIQTWQRPEDSGSQTVMQSQVMKNVRMISPQ
                     ETEVASMMEGMIKVVAEYRNTNNAIGYTFRYYATQMNADKNIKLLAINGIAPTAENIR
                     NGKYPYIVDAFMVTRENTTSETQKLLEWFLTPQGQSLVEDVGYVPMYKTLP"
BASE COUNT         2372 a         2359 c         2262 g         2195 t
ORIGIN      
        1 ttgtttgctg tcgtgataat tgcaaccgtc gtgccagaaa attaaataag cagattaatt
       61 ttttaattca ttgtttttaa ataggttaaa tctttacgtc cgtaaacgga gattttccac
      121 aaaaccaatt caccgataat gaaatatcgt cttttataag gatatctaag atgcgtaaaa
      181 cagtggcttt tggctttgtc ggtaccgtgc tggattatgc cggacgcggc agtcagcgct
      241 ggtcgaaatg gcgtccgacg ctttgtttat gccagcaaga atcgttagtc atcgatcggc
      301 tggaattgtt gcacgacact cgctcgcgct cgctattcga aacgcttaaa cgcgatatcg
      361 ccagcgtttc gccagaaaca gaagtcgtgg gcgttgagat tgaactgcat aacccgtggg
      421 atttcgaaga ggtttacgcc tgcctgcatg atttcgcccg tggttacgag tttcagccag
      481 aaaaagaaga ctatttaatt cacatcacca ccggcaccca cgtcgcgcag atttgctggt
      541 ttctgctggc ggaagcgcgt tacctgcccg cccggctgat acaatcttca cctccgcgca
      601 aaaaagaaca gccgcgcggc gcaggtgaag tgacgattat cgatctcgat ttaagccgtt
      661 ataacgccat cgccagccgc tttgccgagg aacgccagca aacgcttgat tttcttaagt
      721 ccggcattgc cacgcgtaac ccccacttca accgcatgat tgagcagatc gaaaaagtgg
      781 cgatcaaatc ccgcgcgccg attctgctta acggtccaac cggcgcgggc aagtcatttc
      841 tggcgcgacg catcttcgag ttaaaacagg cgcggcatca gtttagcggc gcgtttgtgg
      901 aagtgaactg cgccaccctg cgcggcgata ccgccatgtc gacgctgttt ggtcatgtaa
      961 aaggcgcgtt taccggggcg cggtaatctc gtgaaggttt attacgcagc gccaacggcg
     1021 gaatgttgtt tcttgatgag attggcgaac tgggcgcaga cgaacaggca atgctgctaa
     1081 aagccattga agagaaaacc ttttacccgt ttggcagcga tcgccaggtg agcagtgatt
     1141 ttcagcttat cgccggaacg gtgcgcgatt tgcgccagct ggttgccgaa ggcaaatttc
     1201 gcgaagatct gtacgcgcgg atcaatctct ggaccttcac cctgccgggt ctacgccagc
     1261 gccaggaaga tattgaaccg aaccttgatt atgaagtgga gcgccacgcc acactcactg
     1321 gcgacagcgt gcgttttaac accgaagcgc ggcgcgcctg gttggctttt gcgacctctc
     1381 cccaggcaac atggcgcggt aactttcgcg aactttctgc cagcgtcacg cggatggcca
     1441 cctttgccac tagcggacgc atcactctgg acgtggttga agatgagata aaccgtctgc
     1501 gctataactg gcaggagagc cgcccctccg cgcttacggc gttgctgggc gcagaggcag
     1561 aaaacatcaa tctcttcgac cgtatgcaac tggaacacgt tatcgctatc tgccgctagg
     1621 caaagtcgct ttctgcagcc ggacgacagc tttttgatgt ttcgcgccag ggcaaagcca
     1681 gcgtcaatga cgcggatcgg ctacgcaaat acctggcgcg ttttggtctg acgtgggaag
     1741 ccgtgcagga tcagcacagc tccagttgaa tatgatggtc cgtcagcacc tgcattacgc
     1801 ttactggcgg cggggcgtcg gtgtagacgg catctaccat gctgatgctg cccatattga
     1861 ccatcgcgtt acggccaaat ttcgagtgat cgacaaccag cataacgtgg cgcgagttct
     1921 caataatggc gcgcttggtg cgaacttcgt ggtaatcgaa ctccagcagc gagccgtcgc
     1981 tatcgatgcc gcttatcccc agaatgccga aatcaaggcg gaactgggag ataaaatcga
     2041 gcgtcgcttc gccaatgatc ccgccatcgc ggctgcgtaa ttcgccaccg gcgagaataa
     2101 tgcggaaatc ttcttttacc atcaacgtgt tagcaacatt gagattgttg gtgacaatac
     2161 gcaaattgct gtgattaagc agcgcgtgcg ccaccgcttc cggcgtggtg ccgatatcga
     2221 taaacagtgt cgagccgttg gggatttgct ccgccacttt gcgggcaatc ctctcttttt
     2281 cttcggtctg ggtggctttg cgatcgtgcc acggcgtgtt aaccgaactg gaaggcagcg
     2341 ccgcaccgcc atggtgacgc aggatcaggt tttgctccgc cagctcattg aggtcgcggc
     2401 gaatagtctg cgggctgacg gagaaatgct ctaccagctc ttcagtactg acataaccct
     2461 gctgtttaac cagttcgata ataccgttgt gacgttgtgt ttgtttcatt tataaatccc
     2521 tggaattatt ttcgttttcg cgcattgagc gaatcaacaa aagccatcgc taaacccacg
     2581 gctaacccgg cgatgtgtgc tccgttcgcc atcgacatcc caaacaaatc aaaccatccg
     2641 gcgacaatcc agatcagcgc aaagataatt aacccacgtt gcaggtaaat gccactttgc
     2701 ggatcgcgtt caccacgtag ccagacgtag cccatcagcg catacaccac gccagaaagc
     2761 ccgccaaacc acggcccgct gaatttttgc tgcacatagc cgcttaacag ggcgctaatg
     2821 agagtaataa caattagctt accgctaccg aggcgttttt ccaccgcacc gccgagatac
     2881 caccaccaga gcaggttaaa gaggatatgc atcagcgaga agtgcattaa cgcgtgggtg
     2941 aagtaacgcc agaactcaaa tttcagtgct ggatcgaatg gccaggccag ccataacatc
     3001 acttcctgat cgccgagaat ttgcatggca ataaacacca ccacgcaggc gatcatcatc
     3061 acccaggtca ccggacctgc gcgttcacgc aaggcggcaa agaaaggata acggcgataa
     3121 tgcaggccac tgccggtatg gcctgcctgc cagctggccg ccagataacg cggatctgcc
     3181 gggttttcga gaaaacgcgc cagctccgcc cgtacgcgct cggcctggga ctcatccgcc
     3241 agccagacat cgctttggtt atgttgttga atcgtgagga taacaccctg cgtcgccatg
     3301 taatcaacaa acgcctgcgc cacgcggggg ttagcaaaag aggtaatcat caacatcgtt
     3361 gctgtcgctt attccacaca aaaggggaca gtataaagcg ttacgcgccg tacgccacct
     3421 ctgcgggaaa ctgacgttgc caggcttcaa agccgccgtc aatgctatag accacatcgt
     3481 agccctgttg cagcagatac tgcgccgcgc ctttgctgct attgccgtga taacacatca
     3541 ccatcaccgg agtgtcaaag tcgttatcac gcataaacgc gcccagcgtg tcgttggtta
     3601 aatggaaagc ctgcaccgca tgccccatag cgaaactctg tggatcgcga atatcgacca
     3661 gcaccgcctc tttttcctgc aacttctggt gcgcgtcggc aacgttaata cattcgaact
     3721 gatccatgcg tctctctttc tttacaaaca agtgggcaaa tttaccgcac agtttacgtc
     3781 gaagcggcag ataaacgcca taatgttata catatcactc taaaatgttt tttcaatgtt
     3841 acctaaagcg cgattctttg ctaatatgtt cgataacgaa catttatgag ctttaacgaa
     3901 agtgaatgag ggcagcatgg aaaccaaaga tctgattgtg ataggtggcg gcatcaatgg
     3961 tgctggtatc gcggcagacg ccgctggacg cggtttatcc gtgctgatgc tggaggcgca
     4021 ggatctcgct tgcgcgacct cttccgccag ttcaaaactc attcacggtg gcctgcgcta
     4081 ccttgagcac tatgaattcc gcctggtcag cgaggcgctg gctgaacgtg aagtgctgct
     4141 gaaaatggct ccgcatatcg ccttcccgat gcgttttcgc ctgccacatc gtccgcatct
     4201 gcgcccggcg tggatgattc gcattggtct gtttatgtac gatcatctgg gtaaacgcac
     4261 cagcctgccg ggatcaactg gtttgcgttt tggcgcaaat tcagtgttaa aaccggaaat
     4321 taagcgcgga ttcgaatatt ctgactgttg ggtagacgac gcccgtctgg tactcgccaa
     4381 cgcccagatg gtggtgcgta aaggcggcga agtgcttact cggactcgcg ccacctctgc
     4441 tcgccgcgaa aacggcctgt ggattgtgga agcggaagat atcgataccg gcaaaaaata
     4501 tagctggcaa gcgcgcggct tggttaacgc caccggcccg tgggtgaaac agttcttcga
     4561 cgacaggatg catctgcctt cgccttatgg cattcgcctg atcaaaggca gccatattgt
     4621 ggtgccgcgc gtgcataccc aaaagcaggc ctacattctg caaaacgaag ataaacgcat
     4681 tgtgttcgtg atcccgtgga tggacgagtt ttccatcatc ggcaccaccg atgtggaata
     4741 caaaggcgat ccgaaagcgg tgaagattga agagagtgaa atcaattacc tgctgaaagt
     4801 gtataacacg cactttaaaa agcagttaag ccgtgacgat atcgtctgga cctactccgg
     4861 tgtgcgtccg ctgtgtgatg atgagtccga ctcgccgcag gctattaccc gtgattacac
     4921 ccttgatatc catgatgaaa acggcaaagc accgctgctg tcggtattcg gcggtaagtt
     4981 gaccacctac cgcaaactgg cggaacatgc gctggaaaaa ctgacgccgt attatcaggg
     5041 cattggcccg gcatggacga aagagagcgt gctgccgggt ggtgccattg aaggcgaccg
     5101 cgacgattac gcagctcgtc tgcgccgtcg ctatccattc ctgactgaat cgctggcgcg
     5161 tcattacgca cgcacttacg gcagcaacag cgagctgctg ctcggcaatg cgggggcgat
     5221 aagcgacctc ggggaggatt tcggtcatga gttctacgaa gcggaactga aatacctggt
     5281 ggatcacgaa tgggtccgcc gcaccgacga tgccctttgg cgtcgcacaa aacaaggcat
     5341 gtggctgaat gcggatcaac aatctcgtgt gagtcagtgg ctggtggaat atacgcagca
     5401 gaagttgtcg ctggcgtcgt aaggatgatg cccggttgaa gtgcaaccgg gctatttgga
     5461 ttccttagac tattcattgt aaataattga ataacggccc ctgagctaaa atcgccaaac
     5521 ttaagcacgt tacccaatcg ccccacttct tcctctggtt tctatttttt ctgcaccaat
     5581 ggcaataata tcccaatgca aaaccaagct gtgcactaat cgggccgaca aaatcagtcc
     5641 aaggctgcgg ccaaaaaggg gaaaaattca ggggaggcca gacaaacgtc agaaagaaat
     5701 ttcggatatt ttccagcaac gctgatttct ctggagaaat ataaagaatg aaattcgaga
     5761 gaattaacca gaaatagacc ggcgaaaaga ccagccagca atgcagtctg ttttttaatt
     5821 caggaggcgt tctgcgggag aattgccaac cgatgccagc cattacactg aaaaaaagtg
     5881 catattcaac cattaactca agttgtaatt cctgaagaga aatacgcaca accaggagcg
     5941 gaaaggagaa tattatttct gccagaaaag ttaacgtgaa tataagcacg gctaagacaa
     6001 taaccgtcgg ccataccaat aatggcacga taacaaaacc gagaattaca gggggaatat
     6061 aaagccggaa tttaatcatt tttattttcc tggtgattcg gatgatgcgt catacatcgc
     6121 gcataaacac tcatcaatta tccagtcatc ttcccagacc tgatattctg tcattggttc
     6181 gggctgtttt agcgggttct caatcgcttg ccgcgctctt tctttcagta aaggatgatt
     6241 atttctagcc caggcattga tttgggtggt aatggcgtta tcgatatcgt cctggttatc
     6301 tctgatataa tcctcacaat atctgttcaa taaacaggca agagaaacgg tcgaccaaaa
     6361 gccgggaact gaattaatgg catccagcaa tggcgcaaga gcgatatcgg cacggaaaaa
     6421 catttccagc atatactgta attcgacagc ttcatcgccg gatacccaat cagtcatata
     6481 ctggcaggca aatcgctgta aaattgcccg ttcatccgca cgccagtttt ctttgttggc
     6541 aaggttgata cggcgcagag aaaaaactac gtgaatattg tcaaaatgcc cctgtgcaac
     6601 aaattccagt aaacgcggca gaaaatagcg gacctcatcg ctattttgcg gatcaggccc
     6661 tgggctacta ttccaggcat tgattaagga atacgggatc gctcgtaacg atgtactccg
     6721 taatgccttt tgttcctgct gactcaggca atattcacaa caaaccacaa atttctgcgg
     6781 cacccgataa cgacgaaagg tgtgataaat ctgctcaatt aatgcctgca tggcggggct
     6841 gggttgcata gttaccccct cgcccgctga tcgcgtgtaa acggttgata gacaaaacga
     6901 gcattcgccg cgttcaggcg aataagggcg ctacgcgaaa gggaaaagct ggggcgaatg
     6961 caactattgc agtctcgaca gctttcctct gcgcaatttt ccactgagta atgacgcgtg
     7021 tagagtttca gttcggcctg cttatcgcga aatccgcgta aactgttatt gttatgaaat
     7081 ttccaggcat ctttaaagac ctgaaagata tagacatcct caatccagga gtcacatttt
     7141 ttaagcgggc gctttgctat tatcacccgc tcatccggat ttagcatcgc cgtaaattgt
     7201 acggtgacgt catttgtcgt gttcaggtca cattgaacat ctggtacatc actgatatag
     7261 gggtcaaatc caaaatcagc accacaggca caaagacggc taatggtggc ggtatcgagg
     7321 gaaaatcccg gccatacctt gctggaggtt aagccaatat ttaatgtgac atcatatcga
     7381 agcaatccct caagaccgtc agcaccgacg aacttttccc cttcatccaa ggcctggtta
     7441 ataagttgct gaacaatgcc ttcatcccag tatgccgatt tgcagttggc aatcgtaata
     7501 tccacgcctg tgaagggatc agtaccggca aggcttgcaa ataatgtatt aactaattcc
     7561 atttttatta tccagcttta aggtagcgtc ttatacatcg ggacataacc tacatcttcc
     7621 accaaactct gtccctgcgg cgttaaaaac cactccaaca atttttgtgt ctctgacgtc
     7681 gtattttccc gcgttaccat aaaggcatcg acgatatagg gatatttgcc gttgcgaatg
     7741 ttttcggcag tcggtgcaat accgttaatc gctagcagtt ttatattttt atcggcgttc
     7801 atttgtgttg cgtaataacg gaaggtgtaa cctattgcgt tgtttgtatt acggtattcg
     7861 gcaacgactt taatcattcc ctccatcata cttgccactt ctgtttcctg cggcgagatc
     7921 atgcggacat ttttcatgac ctgggattgc atcaccgtct ggctaccaga gtcttccggg
     7981 cgttgccagg tctggatctc ctgatcgtta ccgccaacag tacgccagtt ggtaattgca
     8041 ccactgaaga tgtcacgcac ctgttgttca gtcagggaat taaccggatt atccgcattg
     8101 acgatgaaaa caaacgcttc acgggcaaaa ggcgtgtaca gcaaagtaac gcccgattcc
     8161 tcagcgcgtt ttttctgccc accggaaggt tgcgccacaa aaataatatc agcatcacct
     8221 ttaacaatcc tgttatacgc atcgggggta cgagagctct ccagatattc cctggtgtga
     8281 aaatcttccg gtataacgct taaagcataa aatgcagagg cataaattgg gtacgccgcc
     8341 gtagcaccat cgagtcgcgg ccagttttgc gtaaactgaa tttgcggttt accacgcagt
     8401 ggtgtcagtt gattattgag tttatcgggt cgccaggccc aggtatcaat attttcgcgc
     8461 acaccgacac cgggattgag ctgtttatat aaataaccct gataaccagc gacaaaacca
     8521 agcatgagaa ggataaacag gagggaatta cggcaacgta gcccggatgt accggttaaa
     8581 ggatgtctgg ttggccggcc gtaccccagc gaaaaactga tatgcgcgcc aacgggtata
     8641 atgactatcc accagtactc acctccgact actgagacta acaaaacgcc cagccagggg
     8701 agcataagat cagccaacca acccgatccc atctggtgaa attgataatc tgccagatgc
     8761 agcgcgatta tccagccaac caggattaat aaaccgggta ctaaaaaaag acattgtcgc
     8821 cagacggcta tttctctttt ttctggtgca ctatcgcctg cccatactcg ccccgagaaa
     8881 aatagcatta atgcgcccag cgccagcata aaaatgttga gcagaaggta acttgttggc
     8941 gtgataagga tattacaaac ttcaataata ccggcaagtc cgacacccag catagcaaca
     9001 accgccgcca aaaattgcgt cagtatgggg atgccgaaaa aagtcattac cagcgaggac
     9061 aaaatccatt ttctgttttg cattattctt tccattcttt ttgaatggtg aaattatact
     9121 ccccgagtcc ccttgcccct tctggacact tttccgaaat gatggcggaa aaaaacggga
     9181 ccctttgg
//
LOCUS       DADMKF010000153         9129 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.156, whole genome shotgun sequence.
ACCESSION   DADMKF010000153 DADMKF010000000
VERSION     DADMKF010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9129
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.156"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(270..1043)
                     /locus_tag="JNC24_003369"
     CDS             complement(270..1043)
                     /locus_tag="JNC24_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308281.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HAY9160044.1"
                     /translation="MHDQPLLRMSLPSIPSFVLSGLLLLCLPFSSFACATTSHISFSY
                     AARQRMQNRARLLKQYQTHLKKQASYIVEGNAQSRRALRQHNREQIKQHPEWFPAPLK
                     ASDRRWQALAENNHFLSSDHLHNITEVAIHRLEQQLGKPYVWGGTRPDQGFDCSGLVF
                     YAYNKILEAKLPRTANEMYHYRRATIVANNDLRRGDLLFFHIHSREIADHMGVYLGDG
                     QFIESPRTGETIRVSRLAEPFWQDHFLGARRILTEETIL"
     gene            1229..1489
                     /gene="dinJ"
                     /locus_tag="JNC24_003370"
     CDS             1229..1489
                     /gene="dinJ"
                     /locus_tag="JNC24_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin DinJ"
                     /protein_id="HAY9160045.1"
                     /translation="MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVARE
                     KALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDLFDKLGI"
     gene            1492..1770
                     /gene="yafQ"
                     /locus_tag="JNC24_003371"
     CDS             1492..1770
                     /gene="yafQ"
                     /locus_tag="JNC24_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafQ"
                     /protein_id="HAY9160046.1"
                     /translation="MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLP
                     AVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLRFERTGTHAALFG"
     gene            1926..2666
                     /locus_tag="JNC24_003372"
     CDS             1926..2666
                     /locus_tag="JNC24_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HAY9160047.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVVN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            complement(2637..3404)
                     /locus_tag="JNC24_003373"
     CDS             complement(2637..3404)
                     /locus_tag="JNC24_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HAY9160048.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRQKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(3610..4188)
                     /gene="lpcA"
                     /locus_tag="JNC24_003374"
     CDS             complement(3610..4188)
                     /gene="lpcA"
                     /locus_tag="JNC24_003374"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HAY9160049.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            4428..6872
                     /locus_tag="JNC24_003375"
     CDS             4428..6872
                     /locus_tag="JNC24_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HAY9160050.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLAIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYAHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMAVLDGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDDLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALAKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            complement(6915..7388)
                     /gene="ivy"
                     /locus_tag="JNC24_003376"
     CDS             complement(6915..7388)
                     /gene="ivy"
                     /locus_tag="JNC24_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-lysozyme inhibitor"
                     /protein_id="HAY9160051.1"
                     /translation="MGRISSGGMMFKTITTVAALVIATSAMAQDDLTISSLAKGETTK
                     AAFNQMVQGHKLPAWVMKGGTYTPAQTVTLGDDTYQVMSACKPHDCGSQRIAVMWSEK
                     SNQMTGLFSTIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            7542..8312
                     /gene="yafV"
                     /locus_tag="JNC24_003377"
     CDS             7542..8312
                     /gene="yafV"
                     /locus_tag="JNC24_003377"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HAY9160052.1"
                     /translation="MPGLKITLLQQPLVWMDGPANLRHFDRQLEGITGRDVIVLPKMF
                     TSGFAMEAAASSLAQDDVVDWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAI
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHQATRIDAELSMMALREYREKFPAWQDADEFRLR"
     gene            complement(8354..>9129)
                     /locus_tag="JNC24_003378"
     CDS             complement(8354..>9129)
                     /locus_tag="JNC24_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HAY9160053.1"
                     /translation="RKGAIHVISAFSTMHSLVLGQIKTDEKSNEITAIPELLNMLDIK
                     GKIITTDAMGCQKDIAEKIQKQGGDYLFAVKGNQGRLNKAFEEKFPLKELNNPEHDSY
                     AISEKSHGREEIRLHIVCDVPDELIDFTFEWKGLKKLCVAVSFRSIIAEQKKEPEMTV
                     RYYISSADLTAEKFATAIRNHWHVENKLHWRLDVVMNEDDCKIRRGNAAELFSGIRHI
                     AINILTNDKVFKAGLRRKMRKAAMDRNYLASVLAESGLS"
BASE COUNT         2196 a         2284 c         2349 g         2300 t
ORIGIN      
        1 tttgttctcc ttaattatgt aaggagtatt tgatcatgca taagcattaa aaaacagttt
       61 caagtaatga ggaatatctc aaattttaaa cataaaatgc gaattattta gtacaaaaag
      121 tgtacttctg atgatctttc cctgggtgat ggaataacag gaaagcggct cgcaaaaacg
      181 cacagatttg taggcatgat aagacgcgtc agcgtcgcat caggcatctg cgcaccaccg
      241 ccggatgcgg cgtaaacgcc ttatccgtcc tacaaaatcg tctcttccgt caatatccgc
      301 cgcgcgccca aaaaatgatc ctgccagaaa ggttcagcta accgacttac ccgaatggtt
      361 tccccggtgc gcggtgactc aataaactga ccgtcgccca gatacacccc catatggtcg
      421 gcaatctcgc ggctgtggat atggaaaaac agcaaatctc cccggcgcag gtcgttattc
      481 gccacaatgg ttgcccgacg gtagtgatac atctcattgg ccgtacgcgg gagcttcgcc
      541 tcgaggatct tgttgtaggc ataaaaaact aacccactac aatcgaagcc ttgatcgggc
      601 cgcgtgccgc cccagacgta aggcttgcca agctgctgct ccagacggtg aatcgccact
      661 tcggtgatgt tatgcaggtg atcgctgctt aaaaagtggt tgttttccgc cagcgcctgc
      721 cagcgtctgt cactcgcctt gagcggcgca ggaaaccatt ctggatgctg ttttatctgc
      781 tcccggttgt gctggcgcag cgcccttctg ctttgggcat tgccttccac aatgtagctg
      841 gcctgttttt tcagatgggt ttggtactgt tttaataaac gcgcacggtt ttgcatccgc
      901 tggcgggcgg cgtagctgaa agagatatgt gatgtggtgg cgcaggcaaa tgaagaaaac
      961 ggcaagcaaa gcaacagtaa tcccgacaac acaaaagagg gaatcgacgg caaagacatt
     1021 ctcaataatg gctggtcatg cattgatgtt gcagtgtatc ctggcccgta cctgaacaaa
     1081 cttaataaac cattattata aaattaataa atatatttca gtttattatg taaaaacatc
     1141 cgtttattaa attactcatg gattgtcgct gttgctcatt tgagctacaa ttcaagctga
     1201 ataaatatac agcacaggag atcccccaat ggctgctaac gcgtttgttc gcgcccgaat
     1261 cgatgaagat ctgaagaatc aggcggcgga cgtactggcc gggatggggc tgaccatctc
     1321 tgacctggtt cgcataaccc tcacaaaggt cgcgcgtgaa aaggcattgc cgtttgattt
     1381 acgcgagcct aatcaattaa ccattcaatc aatcaaaaac agcgaagctg gcgttgatgt
     1441 tcataaggcc aaagacgctg atgatttatt tgataaatta ggaatttaaa tatgattcaa
     1501 agggatattg aatactcggg acaattttca aaggatgtaa aacttgcaca aaagcgtcat
     1561 aaggatatga ataaattgaa atatcttatg acgcttctta tcaataatgc tttaccgctt
     1621 ccagctgttt ataaagacca cccgctgcaa ggttcatgga aaggttatcg cgatgctcat
     1681 gtcgaaccgg actggatcct gatttacaaa cttaccgata aacttttacg atttgagaga
     1741 actggaactc acgcggcgct ctttgggtaa agcacgctgc cgatgaaatt tgtaaaccct
     1801 gcgccgacag gcttgcgtaa aactcttttg gtgataatgt gagccttcca aatccagact
     1861 attccgattt gggatctatt ttgccgtcct ggcgtgtgta accgttttat caaggaataa
     1921 gcagtatgcg taaaatcgca ttaattcttg cgatgttgtt gatcccgtgc gtttcgtttg
     1981 ccggtttgct gggcagcagt agctcgacca cgccagtgag caaagagtat aagcagcagt
     2041 tgatgggatc ccctgtctac atccagatct tcaaggaaga acgtacgctc gatctctacg
     2101 tcaaaatggg cgagcaatat cagctgctcg acagttataa aatctgtaaa tattccggcg
     2161 gcttagggcc aaaacagcgt cagggcgatt tcaaaagccc ggaagggttt tatagcgtcc
     2221 agcgtaatca gttaaaacca gacagccgtt actacaaagc gattaatatt ggtttcccca
     2281 atgcctatga ccgtgcgcat ggttatgaag ggaaatacct gatgatccac ggcgattgtg
     2341 tttccatcgg ctgctacgca atgaccaatc agggtattga tgagatcttc cagttcgtta
     2401 ctggtgcgct ggtgtttggt cagccgagcg tgcaagtgag tatttacccg ttccgcatga
     2461 ccgacgccaa tatgaagcgc cataaatatt ccaactttaa ggacttctgg gagcaactga
     2521 agccgggtta cgactacttt gagcaaaccc gtaagccacc gaccgtttct gtggtcaatg
     2581 gtcgttacgt ggtcagcaag ccgttaagcc acgaagttgt gcagccacaa ctggcatcaa
     2641 actacacgct ccccgaggca aaataagcgc cattcgcctg gcataatctt ttgccaggtt
     2701 tcattgcccg tcagcggctg tgttgcaatc accgtgacca catcattcgg tgtggtctgc
     2761 gagctgaagt cgatttccac atcctgatcc agcagcgttg ccacgccaaa cggcgcgcgg
     2821 cgggtgatcc agtgtaaatt agtcgagcaa tacgccatca catagcgccc gtcagaaagc
     2881 agcatgttga aaacgccctt ctgccgcagt tcatccgcca gtgaggcgat atatttaaac
     2941 actgccgcca tgttgcccgg tgtgcgcggg taacgctgcg ttaatttatg caggagccag
     3001 cagaaggctt tttcgctatc ggtttcaccg accgggcgga agttgccggt ttccagtgat
     3061 ttgtagcccg tcagttgtcc gttatgggca taagtccagt tacgccccca taactcacgg
     3121 gtaaacgggt gagtattttc cagcgccacc tcgccccgat tggcctggcg aatatgagcc
     3181 accaccgaac aggattttat cgggtagtcc tggacaagtt tggcgatggg ggaattaaag
     3241 ctgggttgtg gatctttaaa tgtgcgacag cctttacctt cgtaaaaggt aatgccccag
     3301 ccatctttat gtggcccggt tccaccacca cgctgcacca gaccggtgaa actaaagcag
     3361 atatcggtag ggacgttggc gctcatcccg agcaattcgc acatgggtaa cctccacaac
     3421 agcgggagcg catggccccc ggcgaagatt gatttaagaa ttttcaggcc ggatgcggcg
     3481 taaacgtctt attcggccta cggttcgcgt acagatttgt aggcatgata agacgcatca
     3541 gcgtcgcatc aggcatcagc gcacaagtgc cggatgcggc gtaaacgcct tatccggcct
     3601 acgccagact tacttaacca tctctttttc aattaactgg atcaggatat ggatcacttt
     3661 aatgtgaatc tcctgaatgc ggtcggcata accaaagtgc ggaacgcgaa tttcgatatc
     3721 cgccgtgcca gccattttac cgccgtcttt accggtcagg gtgatcactt tcatcccctt
     3781 ctcacgcgcc gctgcgatcg ctttgatcac gtttgcagag ttaccggagg tggagatccc
     3841 cagcagtaca tcgccttcgc gacctaccgc ttcaacgtag cgggagaaaa tatcattgaa
     3901 accgaaatca ttaccgacgc aggaaatatg actaacgtca gaaatagcaa tcgccgggta
     3961 gcccggacgg ttttcacggt agcgaccggt caactcttcg gcaaagtgca tagcgtcgca
     4021 atgggaaccg ccgttgccgc aggaaagcac tttgccaccg gctttaaagc tgtctgctaa
     4081 caggaccgcc gcgcgctgaa tggcgtgaat attggcgtca tcttttaaaa agttagccag
     4141 cgtttccgcc gcttcgttca gttcgttacg aataagatcc tggtacatga ggatatcctt
     4201 cagcataaat gtaatagaca aaatgcagtg taccggatac cgccaaaagc gagaagtacg
     4261 ggcaggtgct atgaccagga ctttttgacc tgaagtgcgg ataaaaacag caacaatgtg
     4321 agctttgttg taattatatt gtaaacacat tgctaaatgt ttttacatcc actacaacca
     4381 tatcatcaca agtggtcaga cctcctacaa gtaaggggct tttcgttatg atgattttga
     4441 gtattctcgc tacggttgtc ctgctcggcg cgttgttcta tcaccgcgtg agcttattta
     4501 tcagcagtct gattttgctc gcctggacag ccgccctcgg cgttgctggt ctgtggtcag
     4561 cgtgggtact ggtgcctctg gccattatcc tcgtgccatt taactttgcg cctatgcgta
     4621 agtcgatgat ttccgcgccg gtatttcgcg gtttccgtaa ggtgatgccg ccgatgtcgc
     4681 gcactgagaa agaagcgatt gatgcgggca ccacctggtg ggagggcgac ttgttccagg
     4741 gcaagccgga ctggaaaaag ctgcataact atccgcagcc gcgcctgacc gccgaagagc
     4801 aagcgtttct cgacggcccg gtagaagaag cctgccggat ggcgaatgat ttccagatta
     4861 ctcatgagct ggcggatctg ccgccggagt tgtgggcgta ccttaaagag catcgcttct
     4921 tcgcgatgat tatcaaaaaa gagtacggcg gactggagtt ctcagcttat gcccagtctc
     4981 gcgtgctgca aaaactctcc ggcgtgagcg ggatcctggc gattaccgtc ggcgtgccga
     5041 actcattagg cccgggcgag ctgctgcaac attacggcac tgacgagcag aaagatcact
     5101 atctgccgcg tctggcgcgt ggtcaggaga tcccttgctt tgcactgaca agcccggaag
     5161 cgggttccga tgcgggcgcg attccggaca ccgggattgt ctgcatgggc gaatggcagg
     5221 gccagcaggt gctggggatg cgtctgacct ggaacaaacg ctatattacg ctggcaccga
     5281 ttgcgactgt gcttgggctg gcgtttaaac tctccgaccc agaaaagctg ctgggtggcg
     5341 cagaagattt aggtattacc tgtgcgctga tcccgaccac cacgccgggc gtggaaattg
     5401 gtcgtcgcca cttcccgctg aacgtaccgt tccagaacgg accgacgcgc ggtaaagatg
     5461 tcttcgtacc gatcgattac atcatcggtg ggccgaaaat ggccgggcaa ggctggcgga
     5521 tgctggtgga gtgcctctcg gtaggccgcg gcatcaccct gccttccaac tcaaccggcg
     5581 gcgtgaaatc ggtagcgctg gcaacgggcg cgtatgctca cattcgccgt cagttcaaaa
     5641 tctctatcgg taagatggaa gggattgaag agccgctggc gcgtattgcc ggtaatgcct
     5701 acgtgatgga tgctgcggca tcgctgatta cctacggcat tatgctcggc gaaaaacctg
     5761 ccgtgctgtc ggctatcgtt aagtatcact gtacccaccg cgggcagcag tcgattattg
     5821 atgcgatgga tatcactggt ggcaaaggca ttatgctcgg gcaaagcaac ttcctggcgc
     5881 gtgcttacca gggcgcaccg attgccatca ccgttgaagg ggctaacatt ctgacccgca
     5941 gcatgatgat cttcggacaa ggagcgattc gttgccatcc gtacgtgctg gaagagatgg
     6001 aagcggcgaa gaacaatgac gtcaacgcgt tcgataaact gttgttcaaa catatcggtc
     6061 acgtcggtag caacaaagtt cgcagcttct ggctgggcct gacgcgcggt ttaaccagca
     6121 gcacgccaac cggcgatgcc actaaacgct actatcagca cctgaaccgc ctgagcgcca
     6181 acctcgccct gctttctgat gtctcgatgg cagtgctgga cggcagcctg aaacgtcgcg
     6241 agcgcatctc ggcccgtctg ggggatattt taagccagct ctacctcgcc tctgccgtgc
     6301 tgaagcgtta tgacgacgaa ggccgtaatg aagccgacct gccgctggtg cactggggcg
     6361 tacaagatgc gctgtatcag gctgaacagg cgatggatga tttactgcaa aacttcccga
     6421 accgcgtggt tgccgggctg ctgaatgtgg tgatcttccc gaccggacgt cattatctgg
     6481 caccttctga caagctggat cataaagtgg cgaagatttt acaagtgccg aacgccaccc
     6541 gttcccgcat tggtcgcggt cagtacctga cgccgagcga gcataatccg gttggcttgc
     6601 tggaagaggc gctggtggat gtgattgccg ccgacccaat tcatcagcgg atctgtaaag
     6661 agctgggtaa aaacctgccg tttacccgtc tggatgaact ggcgcacaac gcgctggcga
     6721 aggggctgat tgataaagat gaagccgcta ttctggtgaa agctgaagaa agccgtctgc
     6781 gcagtattaa cgttgatgac tttgatccgg aagagctggc gacgaagccg gtaaagttgc
     6841 cggagaaagt gcggaaagtt gaagccgcgt aattgaataa cggagccgaa aggctccgtt
     6901 tctttatccg ctaattattt aaaattaaag ccatccggat ggttttccag gctgccggtc
     6961 aacgccgcga acagcaccgt tttaccatca atcgaaagcg catcgttcac attcagccag
     7021 gtgagtttct cttgcgacgt tttctcatca atagtcgaga acagccccgt catctgatta
     7081 gatttctcgg accacatcac agcgatacgt tgcgagccac agtcatgcgg tttgcacgcg
     7141 ctcatcacct gatacgtatc atctcccaac gttacggttt gtgcgggagt ataagtaccg
     7201 cctttcatca cccaggcagg cagcttatgc ccttgtacca tctgattaaa tgcagctttg
     7261 gtggtttcgc cctttgcaag gctgctaatg gttaaatcat cctgcgccat tgcactggtg
     7321 gcgatgacca gagcggcgac tgtcgttatt gtcttaaaca tcattcctcc cgagcttatc
     7381 ctgcccatgt tattaacctt ctaaagcctg gcgataacac gataatttgc aaatcagata
     7441 tttccaatat cagcatttgt tatcaattta ttctgcgcta aggcaactgc ctgataaggt
     7501 gttaaagtta taaaaccctt cgcataaagg agttgtacac cgtgcctggt ttgaagatta
     7561 cgcttttgca gcaaccactg gtgtggatgg atggccctgc caacctgcgt cattttgatc
     7621 gtcaactgga aggtattacc gggcgcgatg tgatcgttct accgaagatg tttaccagcg
     7681 gctttgccat ggaagcggca gcttcgtcgc tagcacaaga tgacgtagtg gactggatga
     7741 cagctaaggc gcagcagtgc aatgcgctga ttgcaggcag tgttgcatta caaacggagt
     7801 ctggttcggt taaccgcttt ttgctggttg agccgggcgg cacggtacat ttttatgata
     7861 agcgtcatct gttccgcatg gcagatgagc atctacatta taaagcgggc aatgcgcgag
     7921 tgattgtgga atggcgcggc tggcgtattt tgccgttggt gtgctacgac ttacgttttc
     7981 cggtgtggtc gcgcaatctc aacgattatg acctcgccat ctacgttgcc aactggcctg
     8041 ctccgcgctc tctgcactgg caggcattgc tgacggcccg cgcgattgag aatcaggcgt
     8101 atgtggcggg atgcaatcgc gtcggcagcg atggcaacgg ctgccattat cgcggtgaca
     8161 gccgggtgat taatccacaa ggcgagatta tcgctactgc cgacgcgcat caggcaacgc
     8221 gcattgatgc ggagctgtcg atgatggcat tgcgggaata tagagaaaag tttccggcat
     8281 ggcaggatgc ggatgagttt aggttgcggt gagtgggggt tgtgtttaag gctgggggag
     8341 agtcggggta agactacgaa agcccgctct ccgcaaggac tgacgccaga tagtttctgt
     8401 ccatagctgc ttttcgcatc ttacgtctta accctgcctt gaatacctta tcattcgtca
     8461 aaatattaat agcgatgtgc cgtatccctg aaaataattc tgctgcattt cctcttctta
     8521 ttttgcagtc gtcttcattc attaccacgt ccagacgcca gtgcagctta ttctccacgt
     8581 gccagtggtt tcggattgct gtggcgaact tctctgcggt taaatcagca gaactgatat
     8641 aatatctgac cgtcatttct ggctctttct tttgttctgc tattatggac cgaaaggaga
     8701 ctgccacgca taatttcttc agccctttcc attcaaacgt gaaatcaata agttcatcag
     8761 ggacatcgca aacaatatga agacggattt cttctctgcc gtgactcttt tcactgattg
     8821 cgtaactgtc atgctctgga ttatttaatt ctttcagcgg aaatttttcc tcaaaggctt
     8881 tattaagccg cccctggttt ccttttaccg cgaataaata atcaccgccc tgtttttgta
     8941 tcttctctgc aatatctttc tggcatccca tcgcatcagt tgtgatgatt tttcctttaa
     9001 tatccagcat gttaagaagt tcaggaatgg ctgtaatctc attggatttc tcatccgtct
     9061 taatctgccc gagaaccaga ctgtgcattg ttgagaacgc actaatgaca tgaatcgctc
     9121 ccttgcggc
//
LOCUS       DADMKF010000154         9072 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.157, whole genome shotgun sequence.
ACCESSION   DADMKF010000154 DADMKF010000000
VERSION     DADMKF010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9072
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.157"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(506..1273)
                     /gene="fecE"
                     /locus_tag="JNC24_003379"
     CDS             complement(506..1273)
                     /gene="fecE"
                     /locus_tag="JNC24_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter ATP-binding
                     protein FecE"
                     /protein_id="HAY9160054.1"
                     /translation="MTLRTENLTVNYGTDKVLNDVSLSLPTGKITALIGPNGCGKSTL
                     LNCFSRLLMPQSGTVFLGDNPINMLSSRQLARRLSLLPQHHLTPEGITVQELVSYGRN
                     PWLSLWGRLSAEDNARVNVAMNQTRINHLAVRRLTELSGGQRQRAFLAMVLAQNTPVV
                     LLDEPTTYLDINHQVDLMRLMGELRTQGKTVVAVLHDLNQASRYCDQLVVMANGHVMA
                     QGTPEEVMTPGLLRTVFSVEAEIHPEPVSGRPMCLMR"
     gene            complement(1274..2230)
                     /gene="fecD"
                     /locus_tag="JNC24_003380"
     CDS             complement(1274..2230)
                     /gene="fecD"
                     /locus_tag="JNC24_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter permease
                     subunit FecD"
                     /protein_id="HAY9160055.1"
                     /translation="MKIALVIFITLALAGCALLSLHMGVIPVPWRALLTDWQAGREHY
                     YVLMEYRLPRLLLALFVGAALAVAGVLIQGIVRNPLASPDILGVNHAASLASVGALLL
                     MPSLPVMVLPLLAFAGGMAGLILLKMLAKTHQPMKLALTGVALSACWASLTDYLMLSR
                     PQDVNNALLWLTGSLWGRDWSFVKIAIPLMILFLPLSLSFCRDLDLLALGDARATTLG
                     VSVPYTRFWALLLAVAMTSTGVAACGPISFIGLVVPHMMRSITGGRHRRLLPVSALTG
                     ALLLVVADLLARIIHPPLELPVGVLTAIIGAPWFVWLLVRMR"
     gene            complement(2227..3225)
                     /gene="fecC"
                     /locus_tag="JNC24_003381"
     CDS             complement(2227..3225)
                     /gene="fecC"
                     /locus_tag="JNC24_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dicitrate ABC transporter permease FecC"
                     /protein_id="HAY9160056.1"
                     /translation="MTAIKHPVLLWGLPVAALIIIFWLSLFCYSAIPVSGADATRALL
                     PGHTPTLPEALVQNLRLPRSLVAVLIGASLALAGTLLQTLTHNPMASPSLLGINSGAA
                     LAMALTSALSPTPIAGYSLSFIAACGGGVSWLLVMTAGGGFRHTHDRNKLILAGIALS
                     AFCMGLTRITLLLAEDHAYGIFYWLAGGVSHARWQDVWQLLPVVVTAVPVVLLLANQL
                     NLLNLSDSTAHTLGVNLTRLRLVINMLVLLLVGACVSVAGPVAFIGLLVPHLARFWAG
                     FDQRNVLPVSMLLGATLMLLADVLARALAFPGDLPAGAVLALIGSPCFVWLVRRRG"
     gene            complement(3222..4124)
                     /gene="fecB"
                     /locus_tag="JNC24_003382"
     CDS             complement(3222..4124)
                     /gene="fecB"
                     /locus_tag="JNC24_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter
                     substrate-binding protein FecB"
                     /protein_id="HAY9160057.1"
                     /translation="MLAFIRFLFAGLLLVISHAFAATVQDEHGTFTLEKTPQRIVVLE
                     LSFADALAAVDVSPIGIADDNDAKRILPEVRAHLKPWQSVGTRAQPSLEAIAALKPDL
                     IIADSSRHAGVYIALQQIASVLLLKSRNETYAENLQSAAIIGEMVGKKREMQARLEQH
                     KERMAQWASQLPKGTRVAFGTSREQQFNLHTQETWTGSVLASLGLNVPAAMAGASMPS
                     IGLEQLLAVNPAWLLVAHYREESIVKRWQQDPLWQMLTAAQKQQVASVDSNTWARMRG
                     IFAAERIAADTVKIFHHQPLTVVK"
     gene            complement(4169..6493)
                     /gene="fecA"
                     /locus_tag="JNC24_003383"
     CDS             complement(4169..6493)
                     /gene="fecA"
                     /locus_tag="JNC24_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate transport protein FecA"
                     /protein_id="HAY9160058.1"
                     /translation="MTPLRVFRKTTPLVNAIRLSLLPLAGLSFSAFAAQVNIAPGSLD
                     KALNQYAAHSGFTLSVDASLTRGKQSNGLHGDYDVESGLQQLLDGSGLQVKPLGNNSW
                     TLEPAPAPKEDALTVVGDWLGDARENDVFEHAGARDVIRREDFAKTGATTMREVLNRI
                     PGVSAPENNGTGSHDLAMNFGIRGLNPRLASRSTVLMDGIPVPFAPYGQPQLSLAPVS
                     LGNMNAIDVVRGGGAVRYGPQSVGGVVNFVTRAIPQDFGIEAGVEGQLSPTSSQNNPK
                     ETHNLMVGGTADNGFGTALLYSGTRGSDWREHSATRIDDLMLKSKYAPDEVHTFNSLL
                     QYYDGEADMPGGLSRADYDADRWQSTRPYDRFWGRRKLASLGYQFQPDSQHKFNIQGF
                     YTQTLRSGYLEQGKRITLSPRNYWVRGIEPRYSQIFMIGPSAHEVGVGYRYVNESTHE
                     MRYYTATSSGQLPSGSSPYDRDTRSGTEAHAWYLDDKIDIGNWTITPGMRFEHIESYQ
                     NNAITGTHEEVSYNAPLPALNVLYHLTDSWNLYANTEGSFGTVQYSQIGKAVQSGNVE
                     PEKARTWELGTRYDDGALTAEMGLFLINFNNQYDSNQTNDTVTARGKTRHTGLETQAR
                     YDLGTLTPTLDNVSIYASYAYVNAEIREKGDTYGNLVPFSPKHKGTLGVDYKPGNWMF
                     NLNSDFQSSQFADNANTVKESADGSTGRIPGFMLWGARVVYDFGPQMADLNLAFGVKN
                     IFDQDYFIRSYDDNNKGIYAGQPRTLYMQGALKF"
     gene            complement(6580..7533)
                     /gene="fecR"
                     /locus_tag="JNC24_003384"
     CDS             complement(6580..7533)
                     /gene="fecR"
                     /locus_tag="JNC24_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fec operon regulator FecR"
                     /protein_id="HAY9160059.1"
                     /translation="MNPLLTDSRRQALRSASHWYAVLSGERVSPQQEARWQQWYEQDQ
                     DNQWAWQQVENLRNQLGGVPGDVASRALHDTRLTRRHVMKGLLLLLGAGGGWQLWQSE
                     TGEGLRADYRTAKGTVSRQQLEDGSLLTLNTQSAADMRFDAHQRTVRLWYGEIAITTA
                     KDALQRPFRVLTRQGQLTALGTEFTVRQQDNFTQLDVQQHAVEVLLASAPAQKRIVNA
                     GESLQFSASEFGAVKPLDDESTSWTKDILSFSDKPLGEVIATLTRYRNGVLRCDPAVA
                     GLRLSGTFPLKNTDAILNVIAQTLPVKIQSITRYWINISPL"
     gene            complement(7530..8051)
                     /locus_tag="JNC24_003385"
     CDS             complement(7530..8051)
                     /locus_tag="JNC24_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAY9160060.1"
                     /translation="MSDRATTTASLTFESLYGTHHGWLKSWLTRKLQSAFDADDIAQD
                     TFLRVMVSETLSTIRDPRSFLCTIAKRVMVDLFRRNALEKAYLEMLALMPEGGAPSPE
                     ERESQLETLQLLDSMLDGLNGKTREAFLLSQLDGLTYSEIAHKLGVSISSVKKYVAKA
                     VEHCLLFRLEYGL"
     gene            8343..9040
                     /locus_tag="JNC24_003386"
                     /pseudo
     CDS             8343..9040
                     /locus_tag="JNC24_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT         1987 a         2508 c         2585 g         1992 t
ORIGIN      
        1 cactttttag aaaaatcgat ggtcagaaac tggattagca attccgttcc agggttgctt
       61 ttgatttacg ttggcgtctg atcattgatt tatcctcaaa agcccaacct cattggtaat
      121 gaaccagctc cgtgaatgtc cgctctggca cagagcgaaa ttttttgatc tcccccccct
      181 gaaatctaaa cttagtcatg tcacgttttt gggtttctaa aattttaact tcgcgttttt
      241 cgttgccgta agggttatac agaaatgtcc gttaagcaga gttcaaaatt gattgccgtg
      301 atcacgactg gtttgaaagc cgcgcccaag cctgtacagc tctggtttgc gttgattatg
      361 aacctgtcag cctaaagcaa gcggatggac gatgagtatt ggtaatcttt cagagtccgg
      421 aaaagttcag ccccagtctg aacaggcttg ctggcgccag tccagtttca ttcagtcgtg
      481 gtttggttct tacggcctgt gcaatctacc tcattaggca catcggcctg ccagataccg
      541 gctcggggtg tatttccgct tccacgctga atactgttct cagcaatcct ggggtcatca
      601 cctcttctgg tgtgccttgc gccataacat gtccgtttgc cattaccacc agttgatcgc
      661 agtaccggct agcctgatta aggtcgtgca gcacagcgac caccgttttc ccctgagtcc
      721 ggagttcgcc catcaaccgc atcaggtcca cctggtgatt gatatcaaga taggtggttg
      781 gctcatcaag taatacaacg ggcgtattct gggccaggac cattgccaga aatgcgcgct
      841 ggcgctgacc gccggaaagc tcggttaacc gacgaacggc aagatgattg atccgggtct
      901 ggttcatggc gacattaact cgtgcattgt cttcagcgga gagacgcccc cagagtgaca
      961 gccagggatt acgaccatac gaaaccagct cctggactgt gatcccctct ggcgttaaat
     1021 ggtgctgagg cagcagcgaa agcctgcggg ccaactggcg cgatgagagc atatttatgg
     1081 gattatcgcc gagaaatacg gtgccagact gcggcattaa aagccgcgaa aaacagttta
     1141 acagcgtcga tttcccgcaa ccgttaggac cgatcagggc ggtgatcttc cccgttggca
     1201 gtgagagtga aacgtcgtta agtaccttgt ctgttccgta attgaccgtc agattttcag
     1261 ttcgtaaagt catttatcgc attctcacaa gcaaccagac aaaccacggc gcaccgataa
     1321 tggcggtcag cacgccaacc gggagctcca gtgggggatg aataattctc gccagcagat
     1381 cggcaaccac caacagcaac gcacctgtca gggccgaaac aggcagcagt ctgcggtgac
     1441 gtccaccggt gatgctacgc atcatatgcg gcaccacgag accaataaag ctaatcgggc
     1501 cgcaggcggc cacgccggta gatgtcatgg cgacagctag taacaaagcc cagaatcggg
     1561 tatagggcac cgacacaccg agcgtggtgg cgcgcgcatc gccgagtgca aggaggtcga
     1621 gatcgcggca aaaactcagg ctcagcggca gaaataaaat catcagcggg atggcaatct
     1681 tcacaaagct ccagtcacgg ccccataagc tgccggtcag ccacagcagg gcgttgttca
     1741 catcctgcgg gcgcgagagc atcagataat ccgtcaggct ggcccagcat gcagaaagcg
     1801 ccacgccggt gagtgccagc ttcatcggct ggtgggtctt tgccagcatc ttcagcaata
     1861 tcaaccccgc catgccgccc gcaaaggcca gcagcggcag caccatcacg ggcagtgacg
     1921 gcataagaag tagagccccc acagaggcca ggctggcggc atggttaacg ccgagaatat
     1981 ccggtgatgc cagagggttg cgcacaatcc cctgtatcag cacgcccgcc acggcgaggg
     2041 ctgcaccgac aaacagtgcc agcagcaagc gcggcagtcg gtactccatc aatacataat
     2101 aatgctcgcg tccggcctgc cagtcggtca gcagcgcgcg ccacggcacg gggatcactc
     2161 ccatatggag tgataacagc gcacagcccg ccagggcaag ggtgatgaaa ataaccagcg
     2221 caattttcat cctcgcctcc tcacaagcca gacaaagcaa gggctgccaa tcagcgccag
     2281 cactgcgcct gcgggcagat ctccggggaa ggccagcgcg cgtgcgagta catctgccag
     2341 cagcatcagc gtggccccca gcagcatgct cactggcagt acgttgcgct gatcgaagcc
     2401 tgcccagaag cgcgccagat gtggcaccag cagaccgata aacgccaccg gacctgccac
     2461 gctgacgcac gcaccaacca gaagcagcac taacatattg atgaccaaac gtagcctcgt
     2521 caggttcact cccagcgtat gggcggtgct gtcgctgagg ttgagcaggt tcagttgatt
     2581 cgccagcagc aacacgacag ggactgcagt gaccaccacc ggcaagagct gccagacatc
     2641 ctgccagcgg gcgtgggaca ctccgccagc cagccagtaa aagatgccgt aagcatgatc
     2701 ttcggccagc agcagggtga tgcgggtaag gcccatacaa aaggccgaca gcgcgatacc
     2761 cgcgaggatc agtttgtttc tgtcatgggt atgacgaaat ccgcctcctg cggtcatgac
     2821 cagcagccag ctcacgccgc ccccgcatgc cgcgatgaac gacagagaat agcctgcaat
     2881 cggcgtcgga ctcagcgcgc tggtaagcgc catagccagc gccgcgccgc tgttaatgcc
     2941 gagcagtgaa ggagaggcca ttgggttgtg ggtcagggtt tgcagcagcg tgcccgcgag
     3001 cgccaggctt gcgccgatca gaacggcgac caggcttcgt ggcaaacgaa ggttttgcac
     3061 cagcgcttct ggtagcgttg gcgtgtgtcc aggcagcagg gcgcgggttg catctgctcc
     3121 ggaaacagga atggccgagt agcaaaacag actcagccag aaaataataa taagtgctgc
     3181 aacgggaagc ccccacagca gcaccgggtg ttttatcgcg gtcatttcac aacggtaagc
     3241 ggctgatggt ggaagatttt taccgtgtca gcggcaatac gctctgcagc aaaaataccg
     3301 cgcatccgcg cccaggtgtt actgtcgacc gaagcaacct gctgcttctg cgcggcggtt
     3361 aacatctgcc agagcggatc ttgttgccag cgtttaacaa tgctctcttc gcgatagtgg
     3421 gcaaccagca gccaggcagg attgaccgcc agcagttgct ccaggccgat ggacggcatg
     3481 gacgcacccg ccatcgcagc gggaacgttc agccccagag aggccagcac gctgccggtc
     3541 caggtctcct gagtatgcag gttgaattgc tgttcgcgtg atgtgccaaa ggccacgcgt
     3601 gtccctttgg gaagctggct ggcccactgc gccatcctct ctttatgttg ttccagacgt
     3661 gcctgcatct ctcgcttttt acccaccatt tcgccgatga tagccgcaga ttgcaaattt
     3721 tcagcgtagg tttcgttgcg ggacttaagc agcagtaccg acgcgatttg ctgcaaggcg
     3781 atgtaaaccc ccgcatggcg actgctgtcg gcaatgatca ggtctggttt cagagcggca
     3841 atggcttcca ggctcggctg cgcgcgcgtt ccgacggact gccacggttt caggtgcgca
     3901 cgcacttcgg gcaggatgcg ttttgcatcg ttatcgtcgg caataccgat cgggctgacg
     3961 tccacggcgg ccagcgcatc ggcgaacgag agttccagca ccacaatccg ttgtggcgtt
     4021 ttttcgagtg taaacgtgcc gtgttcgtcc tgaaccgtgg cggcaaaggc gtggctgatc
     4081 accagcagca ggcctgcaaa aagaaaacgg ataaatgcca acataatcac attccagcta
     4141 aaagcccggc aagccgggcg ttaacacatc agaacttcaa cgccccctgc atatacagcg
     4201 tgcgcggctg gcctgcatag atgcctttgt tgttgtcgtc ataagagcgg atgaagtagt
     4261 cctggtcgaa gatgtttttc acaccgaatg ccaggttcag atctgccatc tgcgggccaa
     4321 agtcatacac cacgcgtgcg ccccagagca tgaagccggg aatgcggccg gtactgccgt
     4381 cggcgctctc tttcaccgta ttggcgttat ccgcaaactg gctggactgg aaatcgctgt
     4441 tcagattgaa catccagttt cctggcttgt agtccacgcc cagcgtgcct ttatgtttcg
     4501 gggagaatgg taccagattg ccgtaggtgt cgcctttctc gcggatttcc gcgttcacat
     4561 acgcatagct ggcgtagatg gaaacgttat caagcgttgg cgttagcgta cccagatcgt
     4621 aacgtgcctg cgtttccagc ccggtatggc gcgttttgcc acgtgcagtg acggtgtcgt
     4681 tggtctggtt ggagtcgtac tgattgttaa agttaatcag gaacagcccc atttccgccg
     4741 tcagcgcgcc gtcgtcgtag cgggtaccga gttcccaggt tcgcgctttt tccggttcca
     4801 cattgccgct ttgcacagcc ttgccaatct ggctgtactg tacggtgccg aacgagcctt
     4861 cagtgtttgc ataaagattc cagctgtcag tcaggtgata gagcacgttc aacgccggaa
     4921 gcggtgcgtt atagctcact tcttcgtgcg tgcctgtgat ggcgttgttc tggtatgact
     4981 cgatatgttc gaaacgcata cccggcgtga tggtccagtt gccgatgtcg attttgtcat
     5041 ccagatacca ggcgtgcgcc tcggtgccgg aacgcgtatc gcggtcgtaa gggcttgagc
     5101 cggacggcaa ctgcccgctg ctggtggcgg tgtagtaacg catttcatgc gttgattcat
     5161 tcacatagcg atagcccacg cccacttcgt gcgcggaagg gccgatcata aagatctggc
     5221 tgtagcgtgg ctcaataccg cgcacccagt agttacgcgg cgagagggtg atgcgtttgc
     5281 cttgctccag gtagccgctg cgcagggttt gggtgtagaa cccctgaatg ttgaatttat
     5341 gctggctgtc tggctggaac tggtagccca ggctcgccag cttgcgacga ccccagaagc
     5401 ggtcatacgg gcgggtggat tgccagcgat cggcgtcgta atccgcgcga gacaggccac
     5461 cgggcatgtc ggcttcaccg tcgtaatatt gcagcaggct gttgaaggtg tgcacctcat
     5521 ccggcgcata tttgcttttc agcatcaggt cgtcgatgcg ggtggcgctg tgctcgcgcc
     5581 agtcactgcc gcgcgtgccg gagtagagca gcgcggtgcc aaaaccgttg tccgctgtgc
     5641 cgcccaccat caggttgtgc gtctctttcg ggttgttttg tgaagaggtt gggctgagct
     5701 gaccttccac gcccgcctcg ataccaaagt cctgcggaat ggcacgggta acaaagttca
     5761 ccacgccgcc cacgctctgc ggtccgtaac gcaccgcacc accaccgcgt accacgtcaa
     5821 tggcattcat gttgccgagc gaaacgggag ccagtgaaag ctgcggctga ccgtaagggg
     5881 cgaaggggac ggggatgccg tccatcagga cggtcgagcg gctggcgagg cgcgggttca
     5941 ggccccggat gccaaagttc atcgccaggt cgtggctgcc ggtgccgttg ttttccggcg
     6001 cgctgacgcc agggatgcgg ttaagtacct cacgcatggt ggttgcgccg gttttggcga
     6061 aatcctcacg gcggatcacg tcacgcgcgc cagcatgttc aaatacgtcg ttttcacgcg
     6121 catcacccag ccagtcgccg accacggtca gggcatcttc ttttggtgcg ggcgcgggct
     6181 ccagcgtcca gctgttattt cccagcggtt ttacctgcag tccgctgccg tccagcagtt
     6241 gttgcaggcc gctctcgacg tcgtaatcgc cgtgcaggcc gttgctctgc ttgccgcgcg
     6301 tcaggctggc gtcaaccgag agggtaaatc cgctgtgtgc ggcatactga ttgagcgctt
     6361 tatcgagcga tcccggtgcg atattaacct gtgcagcaaa agcggaaaac gagagaccgg
     6421 ccagcggcag caggctcagg cgaatggcgt taaccaaagg tgttgtttta cgaaaaacgc
     6481 gtaacggcgt cataccttcc ccatcatcat ttttgttgtg ttcagctatg agtcgaacga
     6541 gaagggtaaa aaggacaatc gaaataagaa ttattttcct tacagtggtg aaatgtttat
     6601 ccagtaccgc gtaatagact gaattttaac gggaagcgtt tgcgcgataa cgttcaggat
     6661 cgcatcggta tttttcagcg ggaacgtccc gctcaggcgc agcccggcaa cggcgggatc
     6721 gcagcgcagc acgccgttgc ggtaacgggt tagcgtggct atcacctcac ccagcggttt
     6781 atcgctgaag ctcaggatgt ccttcgtcca gcttgtactc tcgtcatcca gcggtttcac
     6841 tgcgccaaac tcagaggcgc tgaactgcag gctttcacca gcgttcacga tgcgtttttg
     6901 cgcgggggca ctggcgagaa gcacttccac ggcgtgctgc tgcacgtcaa gctgcgtgaa
     6961 attatcctgc tggcggacgg taaattctgt ccctaaagca gtgagctggc cctgacgggt
     7021 caggacacgg aaggggcgtt gcagggcatc tttcgcggta gtaatggcga tttcaccgta
     7081 ccagagccgg acggtgcgct gatgcgcatc aaaacgcata tccgccgcgc tttgggtatt
     7141 cagcgtgagc agggagccat cttccagttg ctgacggctg actgtgcctt tggcggtgcg
     7201 gtaatctgcc cgcagacctt cgccggtttc cgactgccag agctgccagc ctccgccagc
     7261 gccgagcaac agcagtaatc ctttcatcac gtgacggcgg gtgaggcggg tatcgtgcaa
     7321 cgcccggctg gcaacgtcgc caggcacacc gccaagctgg ttgcgcaggt tttcaacctg
     7381 ctgccaggcc cactggttat cctgatcctg ttcataccac tgttgccagc gcgcttcctg
     7441 ttgtgggctg acgcgctcgc cgcttagcac ggcataccag tgggaagctg aacgcagcgc
     7501 ctgacggcgg gaatcggtta acaaaggatt cataacccat actccagacg gaacagcagg
     7561 cagtgctcga cggctttcgc cacgtatttt ttcacggagc tgatggaaac accgagtttg
     7621 tgcgcaatct cgctgtatgt cagaccatcc agttgcgaaa gcagaaacgc ttcacgtgtt
     7681 ttgccgttta gcccgtccag catgctgtcg aggagttgta gggtctcgag ttggctttcg
     7741 cgttcctcag gtgaaggcgc tcccccctcc ggcataagcg ccagcatctc cagatacgct
     7801 ttttccagcg cgtttcggcg aaacaggtcc accatcacgc gtttggcgat agtgcagagg
     7861 aaggagcgag gatcgcggat cgtcgagagc gtttcgctga ccattacccg caaaaaagtg
     7921 tcctgggcaa tgtcatctgc atcaaaagca gactggagtt tgcgcgtcag ccagcttttc
     7981 aaccagccgt gatgtgtgcc ataaagcgac tcgaatgtta aggaagctgt ggtagtggcg
     8041 cggtcagaca tgcggagtgc atcaaaagtt aattatcacg tagccatatt aatatgagaa
     8101 tagttatcat tacaattgga aataaaattg tttccaatag acatttttaa catgttgttt
     8161 ttctaagtgt tataaggtag gtataaaatg ggatggagcc tctgcttctg gcatgtgtcg
     8221 gtcagaatga ctcatgatgt ggtctgctat tattgacatc ctcactgccc taaaggatag
     8281 ggatttcggt aatgctgtca acttactgat ttagtgtatg atggtgattt taaggtgctt
     8341 gcgtggcttc catttccatc agatgtcctt cctgctccgc tactgaaggc gtggtgcgta
     8401 acggcaaaag cactgccgga catcagcgct atctctgctc tcattgccgt aaaacatggc
     8461 aactacagtt cacttacacc gcctctcagc ccggtaagca ccagaaaatc attgatatgg
     8521 ccatgaatgg cgtcggatgt cgcgccagtg cacgcattat gggcgttggc ctcaacacgg
     8581 ttttacgtca cttaaaaaac tcaggccgca gtcggtaacc tcgcgcatac aaccgggcag
     8641 tgatgtgatt gtctgcgctg aaatggacga acagtggggc tacgtcggtg ctaaatcacg
     8701 tcagcgctgg ctgttttacg cgtatgacag gatacggagg acggttgtgg cgcacgtctt
     8761 cggtgaacgc actctggcca cactggagcg tcttctgagc ctgctgtcgg cctttgaggt
     8821 cgtggtatag atgacggatg gctggccgct gtatgaatca cgcctgaagg gaaagctgca
     8881 cgttatcagc aagcgttaca ctcagcgcat tgagcgacat aatctgaatc tgagacaaca
     8941 tctggcaagg ctgggacgga agtcactgtc gttctcaaaa tcggtggagc tgcatgacaa
     9001 ggtcatcggg cattatctga acataaaaca ctatcagtaa gttggagtca ttacctcctc
     9061 ccgaactcaa aa
//
LOCUS       DADMKF010000155         8810 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.158, whole genome shotgun sequence.
ACCESSION   DADMKF010000155 DADMKF010000000
VERSION     DADMKF010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8810
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.158"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(1..766)
                     /locus_tag="JNC24_003387"
                     /pseudo
     CDS             complement(1..766)
                     /locus_tag="JNC24_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
     gene            1045..1998
                     /gene="tal"
                     /locus_tag="JNC24_003388"
     CDS             1045..1998
                     /gene="tal"
                     /locus_tag="JNC24_003388"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HAY9160061.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            2113..2700
                     /gene="mog"
                     /locus_tag="JNC24_003389"
     CDS             2113..2700
                     /gene="mog"
                     /locus_tag="JNC24_003389"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HAY9160062.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAAGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(2735..3301)
                     /gene="satP"
                     /locus_tag="JNC24_003390"
     CDS             complement(2735..3301)
                     /gene="satP"
                     /locus_tag="JNC24_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HAY9160063.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(3450..4163)
                     /gene="msyB"
                     /locus_tag="JNC24_003391"
     CDS             complement(3450..4163)
                     /gene="msyB"
                     /locus_tag="JNC24_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HAY9160064.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKNMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSAYRVT
                     IPAVIQIACLRRMVSATQV"
     gene            complement(4189..4593)
                     /locus_tag="JNC24_003392"
     CDS             complement(4189..4593)
                     /locus_tag="JNC24_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HAY9160065.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKLLGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            4970..6886
                     /gene="dnaK"
                     /locus_tag="JNC24_003393"
     CDS             4970..6886
                     /gene="dnaK"
                     /locus_tag="JNC24_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HAY9160066.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            6975..8105
                     /gene="dnaJ"
                     /locus_tag="JNC24_003394"
     CDS             6975..8105
                     /gene="dnaJ"
                     /locus_tag="JNC24_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HAY9160067.1"
                     /translation="MAKQDYYEILGVSKTAEEREIKKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(8209..8418)
                     /gene="mokC"
                     /locus_tag="JNC24_003395"
     CDS             complement(8209..8418)
                     /gene="mokC"
                     /locus_tag="JNC24_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HAY9160068.1"
                     /translation="MLNTCRVPLTDRKVKEKRAMKQHKAMIVALIVICITAVVAALVT
                     RKDLCEVHIRTGQTEVAVFTAYESE"
BASE COUNT         2333 a         2186 c         2259 g         2032 t
ORIGIN      
        1 aaggtatatc ccgccgcgcc gcctgccgct tcctgccagc caaaagcact ctcaaaaata
       61 gaccaaatga cgtggggaag ttgcccgata tttattacgc aaatcaccat gctggtcagc
      121 acccagatta tcgccatcaa cgggacaaac ccctgcatga gccgggcgac gccatgaaga
      181 ccgcgagtga ttgccagcag agcaaagaca gcgagaataa tgcctgtcac cagcggggga
      241 aaatcaaaag aaaactcagg gctcgggcaa cggcgttcgc ctgaactccg ctgaaaatta
      301 tgccgtaggc gatgagcaaa aagacggcga acagaacgcc catccaacgc atccccagcc
      361 cgcgcgccat ataccatgcc ggtcctccac gaaactgtcc attgacgtca cgttctttat
      421 aaagttgtgc aagggaacat tcggcaaacg aggtcgccat gccgataaac gcggcaaccc
      481 acatccaaaa gacggctcca ggtccaccgg cggtaatagc cagcgcaacg ccggccaggt
      541 tgccgctacc cacgcgcgcc gcaagactgg tacacaatga ctgaaatgag gttaaaccgc
      601 ctggctgtgg atgaatgcta tttttaagac ttttgccaaa ctggcggatg tagcgaaact
      661 gcacaaatcc ggtgcgaaaa gtgaaccaac aacctgcgcc gaagagcagg taaatcatta
      721 ccgatcccca aaggacgctg ttaataaagg agaaaaaatc tggcatgcat atccctctta
      781 ttgccggtcg cgatgacttt cctgtgtaaa cgttaccaat tgtttaagaa gtatatacgc
      841 tacgaggtac ttgataactt ctgcgtagca tacatgaggt tttgtataaa aatggcgggc
      901 gatatcaacg cagtgtcaga aatccgaaac agtctcgcct ggcgataacc gtcttgtcgg
      961 cggttgcgct gacgttgcgt cgtgatatca tcagggcaga ccggttacat ccccctaaca
     1021 agctgtttaa agagaaatac tatcatgacg gacaaattga cctcccttcg tcagtacacc
     1081 accgtagtgg ccgacactgg ggacatcgcg gcaatgaagc tgtatcaacc gcaggatgcc
     1141 acaaccaacc cttctctcat tcttaacgca gcgcagattc cggaataccg taagttgatt
     1201 gatgatgctg tcgcctgggc gaaacagcag agcaacgatc gcgcgcagca gatcgtggac
     1261 gcgaccgaca aactggcagt aaatattggt ctggaaatcc tgaaactggt tccgggccgt
     1321 atctcaactg aagttgatgc gcgtctttcc tatgacaccg aagcgtcaat tgcgaaagca
     1381 aaacgcctga tcaaactcta caacgatgct ggtattagca acgatcgtat tctgatcaaa
     1441 ctggcttcta cttggcaggg tatccgtgct gcagaacagc tggaaaaaga aggcatcaac
     1501 tgtaacctga ccctgctgtt ctccttcgct caggctcgtg cttgtgcgga agcaggcgtg
     1561 ttcctgatct cgccatttgt tggccgtatt cttgactggt acaaagcgaa tactgataag
     1621 aaagagtacg ctccggcaga agatccgggc gtggtttctg tatctgaaat ctaccagtac
     1681 tacaaagagc acggttatga aaccgtggtt atgggcgcaa gcttccgtaa catcggcgaa
     1741 attctggaac tggcaggctg cgaccgtctt accatcgcac cggcactgct gaaagagctg
     1801 gctgagagcg aaggggctat cgaacgtaaa ctgtcttaca ccggcgaagt gaaagcgcgt
     1861 ccggcgcgta tcactgagtc cgagttcctg tggcagcaca accaggatcc aatggcagta
     1921 gataaactgg cggaaggtat ccgtaagttt gctattgacc aggaaaaact ggaaaaaatg
     1981 atcggcgatc tgctgtaatc attcttagcg tgaccgggaa gtcggtcacg ctacctcttc
     2041 tgaagcctgt ctgtcactcc cttcgcagtg tatcattctg tttaacgaga ctgtttaaac
     2101 ggaaaaatct tgatgaatac tttacgtatt ggcttagttt ccatctctga tcgcgcatcc
     2161 agcggcgttt atcaggataa aggcatccct gcgctggaag aatggctgac atcggcgcta
     2221 accacgccgt ttgaactgga aacccgctta atccccgatg agcaggcgat catcgagcaa
     2281 acgttgtgtg agctagtgga tgaaatgagt tgccatctgg tgctcaccac gggcggaact
     2341 ggcccggcgc gtcgtgacgt aacgcccgat gcgacgctgg cagtagcgga ccgcgagatg
     2401 cctggctttg gtgaacagat gcgccagata agcctgcatt ttgtaccaac tgcgatcctt
     2461 tcgcgtcagg ttggtgtgat tcgcaaacag gcgctgatcc ttaacttacc cggtcagccg
     2521 aagtctatta aagagacgct ggaaggtgtg aaggacgctg cgggtaacgt tgtggtacac
     2581 ggtatttttg ccagcgtacc gtactgcatt cagttgctgg aagggccata cgttgaaacg
     2641 gcaccagaag tggttgctgc attcagaccg aagagtgcaa gacgcgacgt tagcgaataa
     2701 aaaaatcccc ccgagcgggg ggatctcaaa acaattagtg ggattcacca atcggcagaa
     2761 cggtgcgacc aaactgctcg ttcagtactt cacccatcgc cagatagatt gcactggcac
     2821 cacagatcag accaatccag ccggcaaagt ggatgattgc ggcgttaccg gcaatgttac
     2881 cgatcgccag cagggcaaac agcacagtca ggctaaagaa aacgaattgc agaacgcgtg
     2941 cgcctttcag cgtgccgaag aacataaaca gcgtaaatac gccccacaga cccaggtaga
     3001 caccaaggaa ctgtgcattt ggcgcatcgg tcagacccag tttcggcatc agcagaatcg
     3061 caaccagcgt caaccagaaa gaaccgtaag aggtgaatgc ggttaaaccg aaagtgttgc
     3121 cttttttgta ctccagcaga ccggcaaaaa tttgcgcgat gccgccgtag aaaatgccca
     3181 tggcaagaat aataccgtcc agagcgaaat aacccacgtt gtgcaggtta agcagaatgg
     3241 tggtcatgcc gaagcccatc aggcccagcg gtgccggatt agccaactta gtgttgccca
     3301 taattcctca aaaatcatca tcgaatgaat ggtgaaataa tttccctgaa taacagtagt
     3361 gttttcaggg cgcggcataa taatcagcca gtggggcagt gtctacgatc ttttgagggg
     3421 aaaatgaaaa ttttccccgg tttacggtgt cagacctgag tggcgctaac catccggcgc
     3481 aggcaggcga tttgtattac ggctggaatt gtcacgcgat aggcactgcc gctgaccgct
     3541 ttaaccccat ttaatgctgc gcccacaggg cctcccagac ccgcgccgcg cagcaaacca
     3601 tgcccaagca cgctcattgc tgcgtgggtg cgtaaaatgc gggtcaattg gctggaaagc
     3661 aaatgcgaca cgccttttgc caataatttg tctttcatca gcagcggcag cagctcttcc
     3721 agctcattca ccctggcatc gaccgcgtgc agaaactcct gcttatgttc ctcgtccata
     3781 ttcttccagg tgttacgcag aaattgttcc agtaactgtt gctcaatctc aaacgtagac
     3841 atctctttgt cggctttcag cttcaatcgt tttgaaacat cgagcaaaat ggcccgatac
     3901 aatttaccgt gtccgcgcag tttgttggcg atactatcgc caccaaaatg ctgtaattct
     3961 ccagcaatca gctgccagtt gcggcgatgt tgctcgggat gcccttccat cgatttaaac
     4021 agttcgttgc gcatcagtac gctggagagg cgagttttgc ctttttcatt atgggtgagc
     4081 aatcgggcga aatttgccaa ctgttcctca ctacaatgct gaagaaaatc cagatctgaa
     4141 tcattcaggt aattaacatt cattttttgt ggtttctata ttctggcgtt agtcgtcgcc
     4201 gataattttc agcgtggcca tatccgatga gttcaccgta tgaccggaaa aggtgatttt
     4261 tgagacgcag cgtttattgt cgttatcact gttaatgttg atccagtcag tggtttgccc
     4321 ttcttttatt tctgaaggaa tattcagact ctgactggcg ctacgggcgg ctttgaaata
     4381 aaccgatgca ccgcttaact gtaaatcgcc atggtcggca gagagttgta tgcgtttcac
     4441 aatgcgacaa acaggaagtt tcagcgccag atcgttggtt tcgttacgcg gcattgcaat
     4501 gacgccgagg agtttatggt cgtttgcctg cgccgtgcag cacagcatca ggctaatcgc
     4561 caggctggcg gaaatcgtaa aaacggattt cataaggatt ctcttagtgg gaagaggtag
     4621 agggatgaat acccactagt ttactgctga taaagagaag attcaggtac gtaatctttt
     4681 ctttttatta caattttttg atgaatgcct tggctgcgat tcattcttta tatgaataaa
     4741 attgctgtca attttacgtc ttgtcctgcc atatcgcgaa atttctgcgc aaaagcacaa
     4801 aaaatttttg catctccccc ttgatgacgt gggttacgac cccatttagt agtcaaccgc
     4861 agtgagtgag tctgcaaaaa aatgaaattg ggcagttgaa accagacgtt tcgcccctat
     4921 tacagactca caaccacatg atgaccgaat atatagtgga gacgtttaga tgggtaaaat
     4981 aattggtatc gacctgggta ctaccaactc ttgtgtagcg attatggatg gcaccactcc
     5041 tcgcgtgctg gagaacgccg aaggcgatcg caccacgcct tctattattg cctataccca
     5101 ggatggtgaa actctggttg gtcagccggc taaacgtcag gcagtgacga acccgcaaaa
     5161 caccctgttt gcgattaaac gcctgattgg ccgccgcttc caggacgaag aagtacagcg
     5221 tgatgtttcc atcatgccgt tcaaaattat tgctgctgat aacggcgacg catgggtcga
     5281 agttaaaggc cagaaaatgg caccgccgca gatttctgct gaagtgctga aaaaaatgaa
     5341 gaaaaccgct gaagattact tgggtgaacc ggtaactgaa gctgttatca ccgtaccggc
     5401 atactttaac gatgctcagc gtcaggcaac caaagacgca ggccgtatcg ctggtctgga
     5461 agtaaaacgt atcatcaacg aaccgaccgc agctgcgctg gcttacggtc tggacaaagg
     5521 tactggcaac cgtactatcg cggtttatga cctgggtggt ggtactttcg atatttctat
     5581 tatcgaaatc gacgaagttg acggcgaaaa aaccttcgaa gttctggcta ccaacggtga
     5641 tacccacctg gggggtgaag acttcgacag ccgtctgatc aactacctgg ttgaagaatt
     5701 caagaaagat cagggcattg acctgcgcaa cgatccgctg gcaatgcagc gcctgaaaga
     5761 agcggcagaa aaagcgaaaa tcgaactgtc ttccgctcag cagaccgacg ttaacctgcc
     5821 gtacatcact gcagatgcga ccggtccgaa acacatgaac atcaaagtga ctcgtgcgaa
     5881 actggaaagc ctggttgaag atctggtaaa ccgttccatt gagccgctga aagttgcact
     5941 gcaggacgct ggcctgtccg tatctgatat cgacgacgtt atcctcgtgg gtggtcagac
     6001 tcgtatgcca atggttcaga agaaagttgc tgagttcttt ggtaaagagc cgcgtaaaga
     6061 cgttaacccg gacgaagctg tagcaatcgg tgctgctgtt cagggtggtg ttctgactgg
     6121 tgacgtaaaa gacgtactgc tgctggacgt taccccgctg tctctgggta tcgaaaccat
     6181 gggcggtgtg atgacgacgc tgatcgcgaa aaataccact atcccgacca agcacagcca
     6241 ggtgttctct accgctgaag acaaccagtc tgcggtaacc atccatgtgc tgcagggtga
     6301 acgtaaacgt gcagctgata acaaatctct gggtcagttc aacctggatg gtatcaaccc
     6361 ggcaccgcgc ggcatgccgc agatcgaagt taccttcgat atcgatgctg acggtatctt
     6421 gcacgtttcc gcgaaagata aaaacagcgg taaagagcag aagatcacca tcaaggcttc
     6481 ttctggtctg aacgaagatg aaatccagaa aatggtacgc gacgcagaag ctaacgccga
     6541 agctgaccgt aagtttgaag agctggtaca gactcgcaac cagggcgacc atctgctgca
     6601 cagcacccgt aagcaggttg aagaagcagg cgacaaactg ccggctgacg acaaaactgc
     6661 tatcgagtct gcgctgactg cactggaaac tgctctgaaa ggtgaagaca aagccgctat
     6721 cgaagcgaaa atgcaggaac tggcacaggt ttcccagaaa ctgatggaaa tcgcccagca
     6781 gcaacatgcc cagcagcaga ctgccggtgc tgatgcttct gcaaacaacg cgaaagatga
     6841 cgatgttgtc gacgctgaat ttgaagaagt caaagacaaa aaataatcgc cctataaatg
     6901 ggtaattata ctgacacggg cgaaggggaa tttcctctcc gcccgtgcat tcatctaggg
     6961 acaatttaaa aaagatggct aagcaagatt attacgagat tttaggcgtt tccaaaacag
     7021 cggaagagcg tgaaatcaaa aaggcctaca aacgcctggc catgaaatac cacccggacc
     7081 gtaaccaggg tgacaaagag gccgaggcga aatttaaaga gatcaaggaa gcttatgaag
     7141 ttctgaccga ctcgcaaaaa cgtgcggcat acgatcagta tggtcatgct gcgtttgagc
     7201 aaggtggcat gggcggcggc ggttttggcg gcggcgcaga cttcagcgat atttttggtg
     7261 acgttttcgg cgatattttt ggcggcggac gtggtcgtca acgtgcggcg cgcggtgctg
     7321 atttacgcta taacatggag ctcaccctcg aagaagctgt acgtggcgtg accaaagaga
     7381 tccgcattcc gactctggaa gagtgtgacg tttgccacgg tagcggtgca aaaccaggta
     7441 cacagccgca gacctgtccg acctgtcatg gttctggtca ggtgcagatg cgccagggat
     7501 ttttcgctgt acagcagacc tgtccacact gtcagggccg cggtacgctg atcaaagatc
     7561 cgtgcaacaa atgtcatggt catggtcgtg ttgagcgcag caaaacgctg tccgttaaaa
     7621 tcccggcagg ggtggacact ggagaccgca tccgtcttgc gggcgaaggt gaagcgggcg
     7681 agcatggcgc accggcaggc gatctgtacg ttcaggttca ggttaaacag cacccgattt
     7741 tcgagcgtga aggcaacaac ctctattgcg aagtcccgat caacttcgct atggcggcgc
     7801 tgggtggcga aatcgaagta ccgacccttg atggtcgcgt caaactgaaa gtgcctggcg
     7861 aaacccagac cggtaagctt ttccgtatgc gcggtaaagg cgtcaagtct gtccgcggtg
     7921 gcgcacaggg tgatttgctg tgccgcgttg tcgtcgaaac accggtaggc ctgaacgaga
     7981 agcagaaaca gttgctgcaa gagctgcaag aaagcttcgg tggcccaacc ggtgagcaca
     8041 acagcccgcg ctcaaagagc ttctttgatg gtgtgaagaa gttttttgac gacctgaccc
     8101 gctaacctcc ccaaaagcct gcccgtgggc aggcttgggt aaaattaggg tgcgttgaag
     8161 atatgcgagc acctgaaaag tggcggggat cactccccgc cgttgctctt actcggattc
     8221 gtaagccgtg aaaacagcaa cctccgtctg gccagttcgg atgtgaacct cacagaggtc
     8281 ttttctcgtt accagcgccg ccactacggc ggtgatacag atgacgatca gggcgacaat
     8341 catcgcctta tgctgcttca ttgctctctt ctccttgacc ttacggtcag taagaggcac
     8401 tctacatgtg ttcagcatat agggggcctc gggttgatag taaaatatca ctcggggctt
     8461 ttctctatct gccgttcagc taatgcctga gacagatagc ctcaagcacc cgccgctatt
     8521 atatcgctct ctttaaccca ttctgtttta tcgattctaa tcctgaagac gcctcgccta
     8581 tttttgtggc gtaatttttt aatgatttaa ttatttaact ttaatttatc tatttatcgc
     8641 aattattgac gacaagctgg attatttttg aaatattgtc ctaacaaata acgccgactg
     8701 acaagaaatt aattattact tttcctaatt aatccctcag aaatcctgac cttaagctat
     8761 gattatcagg gcttagggtc acccgttagc gctgatatga ctttccgtgt
//
LOCUS       DADMKF010000156         8634 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.159, whole genome shotgun sequence.
ACCESSION   DADMKF010000156 DADMKF010000000
VERSION     DADMKF010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8634
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.159"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(144..2687)
                     /gene="mdoH"
                     /locus_tag="JNC24_003396"
     CDS             complement(144..2687)
                     /gene="mdoH"
                     /locus_tag="JNC24_003396"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HAY9160069.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
     gene            complement(2680..4215)
                     /gene="mdoG"
                     /locus_tag="JNC24_003397"
     CDS             complement(2680..4215)
                     /gene="mdoG"
                     /locus_tag="JNC24_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HAY9160070.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPQGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            4610..5767
                     /gene="mdoC"
                     /locus_tag="JNC24_003398"
     CDS             4610..5767
                     /gene="mdoC"
                     /locus_tag="JNC24_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HAY9160071.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            complement(5775..7256)
                     /locus_tag="JNC24_003399"
     CDS             complement(5775..7256)
                     /locus_tag="JNC24_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HAY9160072.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRQMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLSDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEIILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(7198..7731)
                     /gene="ymdB"
                     /locus_tag="JNC24_003400"
     CDS             complement(7198..7731)
                     /gene="ymdB"
                     /locus_tag="JNC24_003400"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HAY9160073.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            complement(7826..8137)
                     /locus_tag="JNC24_003401"
     CDS             complement(7826..8137)
                     /locus_tag="JNC24_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HAY9160074.1"
                     /translation="MFRPFLNSLMLGSLFFPFIAIAGSTAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSVPGKTYSSKALMSGNVKNAQIASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            complement(8258..8590)
                     /gene="csgC"
                     /locus_tag="JNC24_003402"
     CDS             complement(8258..8590)
                     /gene="csgC"
                     /locus_tag="JNC24_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly protein CsgC"
                     /protein_id="HAY9160075.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDVYTIIPEVTLTQSCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQPIALTKLSLNISPEDRVKIVVTVSDGQALHLSQQ
                     WPPSSEKS"
BASE COUNT         2156 a         2248 c         2051 g         2179 t
ORIGIN      
        1 cataacgccg gtatttttat tggctcgttg tttttgccgg atgcgacgtg aacgctttac
       61 ctaacccaca aaagcttgca aaatcaataa attgcatgaa cgatgtaggc ctgataagcg
      121 tagcgcatca ggcaactaca tttttattgc gaagccgcat ccggtttacg caatgccagt
      181 ggattgagct ttatcccttc gtaataactc acccatgaag aatatctctc aggggaattc
      241 cagacgcgga aatgcagacg ggccatcgtc accggatcgc ttagcagcac aaggcgacga
      301 tcgcgattca gcttctctgg cgtctcgttc agcgcctgtt caacgtggcg gtcacgggcg
      361 atttccagca ccttactggc gcggtgacgc gcggtcgcca ttgcggttgc cagagcgtta
      421 aatgacgggt taaacactgc gtgcataaag ccatcgtcaa gggagcgttg gcgattcatc
      481 tcaaggaacc gatcggtatc aaccagcacc tgcggcggtg aatactcttc cgggatcagg
      541 aacagtttcc agcgtttggt gcgcagacca acggtggcac ggctggaaat caccgaaaca
      601 aacggtgaca ggatcaacga gaagacaatc ggtgccagcc agaacaggaa acgcagatcc
      661 agccacgcca tcccaacagc ccacactaac cctaacagca gctgtgagcc gtggcgtttg
      721 aacgcttcac cccaggaagt ggagtcatca tcacgctgcg gtgaattcca caccacttcc
      781 cagccaagga acgcgctgac gacgaagacc gtatggaaca gcatgcgtac cggagccagc
      841 agcacggaaa acagcacttc cagcagcaac gataatgtaa cgcgccagaa gccgccgtat
      901 tctttcgttc ctttgcacca gataagcaaa atgctcaata acttcggcag gaacaacagc
      961 accatggtcg aagcaaaaag tgcaatcgcc agctcaggac gccactgcgg ccacaccggg
     1021 aacaactgcc gtggttgcag gaagtattgc ggttcggtca acgcatgtac tacctgcaat
     1081 gcagtagaga gcgcgaggaa cataaaccac agcggagcgg agagataaga catcacgccc
     1141 gtcaggaaca ccgcacggtg aaccgggtgc atacccttca ccaggaacag acggaagttc
     1201 atcaggttac cgtggcacca tcggcggtca cgttttagct catcaagcaa gttaggcggc
     1261 aattcttcat aagaacccgg gagatcgtaa gcaatccaga ccccccaacc tgcacggcgc
     1321 atcaacgccg cttccacgaa gtcatgtgac aggattgaac cggcaaagga accttcgccc
     1381 ggcagcggag ccagtgcgca gtgctcgata aacggtttca cgcggataat cgcgttatgt
     1441 ccccagtagt gcgactcgcc aagttgccag aagtgcaaac cggctgtaaa cagtggccca
     1501 tacacgcggg tcgcgaactg ctgacagcgc gcatacagcg tatccatacc ggacgctttc
     1561 ggcgacgact gaatgatccc ggcgttcggg ttggcttcca tcaggcgcac cagcccgcac
     1621 aaacaatcac cggtcattac cgagtcagca tccagcacca ccatgtagct gtactggctg
     1681 ccccagcgac ggcagaagtc atcgatatta ccgcttttac gcttcacgcg gcgacggcgg
     1741 cggcgataga aaatctgacc ttcgccaccg acttcagcga taagctccat ccaggctttt
     1801 tgctctgcga cgcagatatc cgggttataa ctgtcactaa gaatgtagac atcaaagtgt
     1861 ttggcattcc cggtggcttt tactgattcc cacgttgcac gcaggccagc aaaaacacgg
     1921 ttcacgtctt cgttacagat aggcatgatc aacgccgtgc gatgctccgg gtttaatggt
     1981 tcatcgccaa ctgttgacgc agatatactg tatttatcgc gaccaataag cagttgcagg
     2041 aagcccatta acgccgtcca gaatccggcg gacacccaac agaacagtac cgcaaagagg
     2101 atcaggatac cggtttgcag catataaggc agaagctgca taaaggaaac ccacaaatcc
     2161 tgaccaacca tatccatagg attaatcagc gcccaaccct gataaggaag aatggtcttc
     2221 atataccagg tcgcgacgac agtttgcgcg agcgtcagga tcaacagaat gtaacggcgg
     2281 atggtaccga cggtacgcca cttttgctcg ctctcctgct cttctttggt caaacgagcc
     2341 agatagcgcg gcgtgacatc gcgtccacgc aggcgatccc agaaacggcc taccgggttg
     2401 gtacgccacg ggtcgggaaa catcgaggag cgttttgctt ctggcatcgc cttcagctga
     2461 tcgcgccctt cgtcgtcttt aattaactgt ccatcagcaa gtgaatctgg ccaggcttgt
     2521 tccagacgcg cctttaccga gccttgcggg gaatcatcct cccgcgccca ggtgcggtgt
     2581 tcggcatcca gcgcctgatg aacggcgcgg atatcagtct tcggcaatgc cgctttctcg
     2641 cttgcggcga tgggcattgc gtcaatgtac tcagttgtct tattcattgg caggtaactg
     2701 gtagctccag gtttcactca acgtctgatc ggcattcacc agcgcagcac gcatttcagt
     2761 ggttttcttg gcatctttca ctttcacacg catcaccaga cgccagcctt tggtaaccgg
     2821 gttatagcgc accgtgcttt caactatctc accattatca ccaatgctgg tttgcgctgt
     2881 gaccggggta tcctctggca gttttttcat ctctgcgccg gtaaaatcga ccacaaaggc
     2941 gatagtaccg tcaggctggc gaatcaggtt cgactgcttc acatcccccg ttgaacgacg
     3001 cgtttgttgc acccatgcgt tatctggcgc atgcagtttg tcttcatcac ggctgaaggt
     3061 gatggtgtat ttaaagttca tctctttacc cggctccggc agctgatccg gcgtccagta
     3121 agcgacgatg ttatcgttgg tttcatcgtt ggttggaatt tccaccagct caacgctgcc
     3181 tttaccccac tcccctttcg gagtcaccca tgcgcttgga cgaagatcgt aacgatcatc
     3241 gagatcttca aagcgggaga aatcacgacc gcgctgcaac agaccgaagc cttgcgggtt
     3301 ttccatcgag aagctgctaa ccgctaaatg tttcgggtta ttcaacggac gccagatcca
     3361 ttcgccatta ccggcatgga tagagagacc gttagagtcg tgcaactccg gacgatagtt
     3421 atttgcaggc gacggttggt tcggcccaaa caggaacata ctggttaacg gtgcaacccc
     3481 cagtttgccg actttatcgc gcagatagat tttcgactgc acatccacaa ccgtgtcacg
     3541 ccctggcata accacgaatt tgtaagcacc tgtcgcgcgc ggcgagtcaa gcaatgcata
     3601 aatggttaaa cgtttatcag tcggttttgg acgctcgatc cagaactctt tgaagcgtgg
     3661 aaattcttcc cccgatggca aggcggtatc aattgccagg ccgcgggcag aaaggccata
     3721 aacctgacct gcaccaatca cgcggaaata gctggccccg agcatgctga cgatttcatc
     3781 gtttttatct ttgctgttga tcgggtaaag cactttaaaa ccggcaaaac caaggtcttt
     3841 taccgtgtct ttgtcatgct gaacatcgcc gaaagtgaaa taatccgggc tgtatttgat
     3901 tcgtttgact gcggtggcag tcacttcatt tattttgacc ggggtatcga agtacatacc
     3961 ctgatggtag aactcgagtt tgaatggggt cttcagattg ttccagtacg ctttgtcatg
     4021 attaaactgg atctgctgat agtccgcgta tttcatatcg cggaaaacgg agggcaagtt
     4081 gcttttgggc gcctcatagc ctttcccggc taaggattga gcttgctttg cgacatcatc
     4141 aatactgaaa gcccagcttg aagatgtata cagggttaac attactgcag cactcaacca
     4201 acgcattttc atcatttgta gtttatgttt cataattagt aaagcacttc cccctttgtg
     4261 tgcttatatc gatccgatct attttaatgg ataaccagcc attccgacaa cagaatcccg
     4321 gtatcgttca gcacgctggc tcatggatta agcacctgat tgcaaccgtt ttcactttcc
     4381 gtgcttctcc taaagacaga agcatcgact tcgtgtaggg tttgccagca attttattta
     4441 tcaaccgtct tacagacgct tccattctgg ttagtggtag cgatgatgct atgcataaaa
     4501 accttgaagc ctgcgcctgg cgcggtcagg tcggtgtcgc tatggcaaca agcctaaagg
     4561 cataacccga cagggataag acgaaaaatc cgagattagt taaccatata tgaatccagt
     4621 acccgcgcaa cgtgaatatt tcctcgactc catccgcgcc tggctgatgt tgttagggat
     4681 cccttttcat atttctttaa tctattcgag ccatacatgg catgtgaata gcgccgaacc
     4741 gtcattgtgg ctgacccttt ttaatgactt catccactcg ttccgcatgc aggtattttt
     4801 cgttatatcc ggctactttt cctacatgct ttttttacgc tatcccttga aaaaatggtg
     4861 gaaagtacgt gtcgaacgtg taggtatccc gatgttaaca gccatcccct tactgacatt
     4921 gccgcaattt attatgctgc aatacgtcaa agggaaagcg gaaagttggc ctgggctgtc
     4981 attgtatgac aaatataata cgttggcctg ggaattaata tcacacctgt ggtttttact
     5041 ggtgttagta gtcatgacga cgctgtgcgt atggatattt aaacgcatca gaaataattt
     5101 agaaaattct gataaaacga ataaaaaatt ctcgatggta aaactatcgg tgattttttt
     5161 atgcctcggc atcggctatg cggtaataag aagaacgatt tttattgtgt atccgcccat
     5221 tctgagtaat ggcatgttca attttattgt catgcaaacg ctattttatt tgccgttctt
     5281 tatcctcggt gcactggctt tcattttccc tcatcttaaa gccttgttta ccacgccgtc
     5341 tcgtggctgt acccttgcag cagcattggc atttgtcgct tatttactca accagcgcta
     5401 tggcagtggc gatgcctgga tgtacgaaac cgagtcggtg atcaccatgg tcctcggtct
     5461 gtggatggtg aatgtggtct tctcctttgg ccaccgtttg cttaacttcc agtcagcgcg
     5521 ggtgacttac tttgttaacg catcgctatt tatctatctg gttcaccacc cgttaacgct
     5581 gtttttcggc gcatacatta caccgcacat cacctccaac tggcttggtt ttctctgtgg
     5641 cctgatattc gtagtaggga ttgcgataat tctgtatgaa attcatttac gcatcccgtt
     5701 actgaagttt ttgttttctg gtaaaccggt tgttaagcgt gagaacgata aagcaccagc
     5761 ccgttaagcc acatttacaa taaccattcc acgggcaata tcgacgccag tctgaccata
     5821 acccgcttcc agaaactggt ggcgggttct tttttgagaa taatctcttt ctttgcatga
     5881 cgatcaaccc agttgatccg tccccacctg tccagacgga gctgccaggc cgcatcatac
     5941 tggctctgaa taaagcgttt atcaattaac tgtgccagcg tttcgctctc tatcacgaag
     6001 cccatttcag tattgagcaa tgttgaacgc ggatcgaaat tgaaagagcc gataaacacc
     6061 gttttaccat cgatgctaaa agttttagca tgcaggctgg ctccggaatt accggttatg
     6121 ccgcgatcgt gtaacgtact actttgttca cgcgtcggct tgagttcata taattccacg
     6181 ccatagcgga gcaatttttt gcgccagcgc gcgtatccgg catggacgac agcaacatcg
     6241 ttagcggcaa gagaattggt taggatcgca atctttaccc cttttctcac catccgtaag
     6301 agttgcgcca cacctgcgcg tgtcggtaca aaataggaag agataatatc gatgcgttca
     6361 ctgggtgagc ccatgatatc ggacaggcgc tgcggtaaca gtgaatgccg ttttgccttg
     6421 ccctcccctt tcgccggatc atcacttaat aaacgtgttt tcgcccagat aagcggcaac
     6481 gttccatcaa ccagatgatt tataaacgga ctggattcca tttggcgtaa gtaacgatgc
     6541 gtcatggcat cgttatgcca ggaggcgggt aactcgatgc gatccgccat ttcaccttcc
     6601 gggacatcca gcacctgctg taagggtgaa accgatttgc aataccagta gcgggcgaaa
     6661 tcatcggcaa cgtcctctac cacgggtcct attgccataa catctaaatc cgaaaaaagt
     6721 ggctcttccc ctgccccaaa ataggcatca ccaatatttc gtcctcccac cagggtaacc
     6781 acgccatcga cagtgaaact tttattgtgc atacggcgat tgagacggga aaagtcggtg
     6841 atataaccaa gcggacgcag caagcgaaac gagaaaggat taaaaagccg gacttcaatg
     6901 cggggatggc tgtcaagcaa gcgtaaaata tcgtcaagtc cgggcgtatt gttgtcgtcc
     6961 agcaacaaac ggacgcgaac gccacgcttt gctgcggcta acagggcgga aaacagtaac
     7021 cgacccgaca tatcgtcctg ccagatgtaa tactgaacat ccagcgtatg ctctgccatt
     7081 tcggccagac gataccgggc ggcaaacgca tccagacttt tctccagagg gaaaagacca
     7141 cactgaccgg gatgttgcga acacagtggc agcaccgcgc tcgccagccg gggcaaatca
     7201 ttcatctcct tgttgggtaa ggagtctttc gtagaggtgg gcgttttctt catcataaca
     7261 gacaaagtat acctgttcgg gtaaagcgtg acgggtaata aattctgaaa cggttttaac
     7321 tgcgatttca gccgctgccg cacgagggta accataaacc ccagtactga ttgcaggaaa
     7381 agccactgac gtatagctgt ttgccgccac cagtcgcagg ctattcagat aggcatcctg
     7441 caaaagctgg tcttcgtttt gttcaccacc acgccagact ggcccgacgg tgtgtactac
     7501 ggctttagcg ggaagatcgc ctgcaagcgt aataacggca tgccccgtag ggcaatcgcc
     7561 ctgctgttgc ctgactttta aacaggcatc cagcagggcc ggacccgctg cgcgatgaat
     7621 ggccccatcg acgccgccgc ctcccattaa tgacggatta gccgcattca caatcacatc
     7681 aacggccagt ttggtaatat caccctgcac aacatgaata cgcgttttca taacgcctcc
     7741 ttacaccttc ttgtttctta agtgtacagc aggctttttg gttaaatcgc atttttctgc
     7801 tcagcaacgt gcgttgttca gaaacttagt tatattcgat gttcattaca gccagctttt
     7861 tctgtttatc aagatactgc acttttactg aggctatttg cgcgttttta acattaccgc
     7921 tcattaacgc tttgctggaa taggttttgc ccggaacaga accattctgt gggcagttca
     7981 tttctacggg tgatgactgg gtgctgacgt cacatgccgg ttccacaatt tggccataaa
     8041 aatggatcac acccccttgc gcggtgcttc ctgcgatggc aataaaagga aaaaacaaac
     8101 tgccgagcat aagagagttt aaaaaaggtc tgaacattta ttctatttcc tcaatgatta
     8161 gtcatccttg agggttgtgt tatccatact ttcaggttgg ctgattcgca gcagaccatt
     8221 ctctccagat tcatcttatg ctcgatattt caacaaatta agacttttct gaagagggcg
     8281 gccattgttg tgataaatga agtgcctgtc catcagaaac agtaacaact attttcaccc
     8341 gatcttccgg ggaaatattt aaactcaact tcgtcaaagc aataggttga ttagcaggca
     8401 atgagagggt cttttcttgc ttcgtctgac tttgccctga actgccttcg cgcagggaca
     8461 atatttgtac tctgcacaga caagattgag taagagtgac ttcaggaata atggtataca
     8521 catccccttg ctgggtcgta ttaaaggtta tctggctgga aagtgccgca aggagtaata
     8581 acgcattcat attcttctcc cgaaaaaaac agggcttgcg ccctgtttct gtaa
//
LOCUS       DADMKF010000157         8534 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.160, whole genome shotgun sequence.
ACCESSION   DADMKF010000157 DADMKF010000000
VERSION     DADMKF010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8534
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.160"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(<1..359)
                     /locus_tag="JNC24_003403"
     CDS             complement(<1..359)
                     /locus_tag="JNC24_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308431.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAY9160076.1"
                     /translation="MSYQPQTEAATSRFLNVEEAGKTLRIHFNDCGQGDETVVLLHGS
                     GPGATGWANFSRNIDPLVEAGYRVILLDCPGWGKSDSIVNSGSRSDLNARILKSVVDQ
                     LDIAKIHLLGNSMGGHSS"
     gene            complement(377..1321)
                     /locus_tag="JNC24_003404"
     CDS             complement(377..1321)
                     /locus_tag="JNC24_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydroxyphenylpropionate/2,
                     3-dihydroxicinnamic acid 1,2-dioxygenase"
                     /protein_id="HAY9160077.1"
                     /translation="MHAYLHCLSHSPLVGYVDPAQEVLDEVNGVIASARERIAAFSPE
                     LVVLFAPDHYNGFFYDVMPPFCLGVGATAIGDFGSAAGELPVPVELAEACAHAVMKSG
                     IDLAVSYCMQVDHGFAQPLEFLLGGLDKVPVLPVFINGVATPLPGFQRTRMLGEAIGR
                     FTSTLNKRVLFLGSGGLSHQPPVPELAKADAHMRDRLLGSGKDLPASERELRQQRVIS
                     AAEKFVEDQRTLHPLNPIWDNQFMTLLEQGRIQELDAVSNEELSAIAGKSTHEIKTWV
                     AAFAAISAFGNWRSEGRYYRPIPEWIAGFGSLSARTEN"
     gene            complement(1323..2987)
                     /locus_tag="JNC24_003405"
     CDS             complement(1323..2987)
                     /locus_tag="JNC24_003405"
                     /EC_number="1.14.13.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid
                     hydroxylase"
                     /protein_id="HAY9160078.1"
                     /translation="MAIQHPDIQPAVNHSVQVAIAGAGPVGLMMANYLGQMGIDVLVV
                     EKLDKLIDYPRAIGIDDEALRTMQSVGLVENVLPHTTPWHAMRFLTPKGRCFADIQPM
                     TDEFGWPRRNAFIQPQVDAVMLEGLSRFPNVRCLFSRELEAFSQQDDEVTLHLKTAEG
                     QRETIKAQWLVACDGGASFVRRTLNVPFEGKTAPNQWIVVDIANDPLSTPHIYLCCDP
                     VRPYVSAALPHAVRRFEFMVMPGETEEQLREPQNMRKLLSKVLPNPDNVELIRQRVYT
                     HNARLAQRFRIDRVLLAGDAAHIMPVWQGQGYNSGMRDAFNLAWKLALVIQGKARDAL
                     LDTYQQERRDHAKAMIDLSVTAGNVLAPPKRWQGTLRDGVSWLLNYLPPVKRYFLEMR
                     FKPMPQYYGGALVREGEAKHSPVGKMFIQPKVTLENGDVTLLDNAIGANFAVIGWGCN
                     PLWGMSDEQIQQWRALGTRFIQVVPEVQIHTAQDNHDGVLRVGDTQGRLRSWFAQHNA
                     SLVVMRPDRFVAATAIPQTLGNTLNKLASVMTLTRPDADVSVEKVA"
     gene            3029..3346
                     /locus_tag="JNC24_003406"
     CDS             3029..3346
                     /locus_tag="JNC24_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAY9160079.1"
                     /translation="MQNNEQTEYKTVRGLTRGLMLLNMLNKLDGGASVGLLAELSGLH
                     RTTVRRLLETLQEEGYVRRSPSDDSFRLTIKVRQLSEGFRDEQWISALAAPLLGDLLR
                     EVV"
     gene            3356..3862
                     /locus_tag="JNC24_003407"
     CDS             3356..3862
                     /locus_tag="JNC24_003407"
                     /inference="COORDINATES: protein motif:HMM:NF013736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HAY9160080.1"
                     /translation="MSTLDVDAMVVRETTHRFSRLSFHRAMVGRRLPLLKTASGLTWL
                     AFCPEQERKELIEMLAARPGDDYQLAREPLKLQAILARARKEGYGQNYRGWDQEEKIA
                     SIAVPLRSEQRVIGCLNLVYMASAMTIEQAAEKHLPALQRVAKQIEEGVESQAILVAG
                     RRSGVHLR"
     gene            3939..5021
                     /locus_tag="JNC24_003408"
     CDS             3939..5021
                     /locus_tag="JNC24_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414879.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAY9160081.1"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAE
                     LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS
                     GVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSII
                     FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG
                     DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT
                     EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNT
                     QTASPRALADSLMQLARQVSRLESGQ"
     gene            5144..8218
                     /locus_tag="JNC24_003409"
     CDS             5144..8218
                     /locus_tag="JNC24_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HAY9160082.1"
                     /translation="MIMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEAR
                     TDRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYT
                     NVTYPITVNPPFVPAENPTGCYSLTFNIDESWLQEGQTRIIFDGVNSAFHLWCNGRWV
                     GYGQDSRLPSEFDLSAFLRAGKNRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHK
                     PSTQISDFHVATHFNDDFSRAVLEAEVQMYGELRDELRVTVSLWQGETQVASGTAPFG
                     GEIIDERGGYADRVTLRLNVENPALWSAEIPNLYRAVVELHTDDGTLIEAEACDVGFR
                     EVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSH
                     YPNHPLWYTLCDRFGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRN
                     HPSVIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVD
                     EDQPFPAVPKWSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGG
                     FVWDWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQ
                     QQFFQFRLSGQTIEVTSEYLFRHSDNELLHWSVALDGKPLASGEMPLDVAPQDKQLIE
                     LPELPQPESAGQLWLTVHVVQPNATAWSEAGHISAWQQWRLAENLSVTLPAAPHAIPQ
                     LTTSETDFCIELGNKRWQFNRQSGFLSQMWIGAEKQLLTPLRDQFTRAPLDNDIGVSE
                     ATRIDPNAWVERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKT
                     YRIDGSGQMAITVDVEVASDTPHPARIGLTCQLAQVAERVNWLGLGPQENYPDRLTAA
                     CFDRWDLPLSDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETS
                     HRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGSYHYQLVWCQK"
     gene            8270..>8534
                     /locus_tag="JNC24_003410"
     CDS             8270..>8534
                     /locus_tag="JNC24_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY9160083.1"
                     /translation="MYYLKNTNFWMFGLFFFFYFFIMGAYFPFFPIWLHDINHISKSD
                     TGIIFAAISLFSLLFQPLFGLLSDKLGLRKYLLWIITGMLVMFA"
BASE COUNT         1940 a         2390 c         2336 g         1868 t
ORIGIN      
        1 gaactatggc cgcccatcga gttgcccagc aggtggattt tggcgatatc cagttgatcc
       61 accacgcttt tcaggattcg tgcattaaga tccgatcgtg aaccactatt aacgatcgaa
      121 tcgcttttgc cccaacccgg acaatccagc aggatcaccc gatagcccgc ctctaccagc
      181 ggatcgatat tgcggctgaa gttcgcccag ccagtagcac ccgggccgga accatgcagc
      241 aggacaacgg tttcgtcgcc ttgtccgcag tcattaaaat ggatgcgcag cgttttaccc
      301 gcttcttcta cattcagaaa acggctggtg gcggcttcgg tttgtggctg ataactcatc
      361 atcttctcct gcatattcag ttctctgttc tggcgcttaa cgagccaaat ccggcaatcc
      421 actccgggat tgggcggtaa taacgccctt cgctacgcca gttgccaaat gcagaaatag
      481 cggcaaaagc ggcgacccag gttttgattt catgtgtcga ctttccggca atggcggaaa
      541 gctcttcgtt actgacggca tccagttcct gtatgcgtcc ctgctccagc aaagtcatga
      601 actggttatc ccaaatcggg ttgagcggat gcagagttct ctgatcctca acaaacttct
      661 cagcggcgct aatcaccctt tgctgacgca attcgcgctc actggcgggt aaatctttcc
      721 cgctccccaa cagacggtcg cgcatatggg catcggcttt cgccagttcg ggcaccggcg
      781 gctgatggga aagcccaccg gaacccagga acagcacgcg tttattgaga gtgctggtga
      841 aacgtccaat ggcttcaccc aacatgcggg tacgctggaa accgggcagc ggcgtggcga
      901 caccgttgat gaacacaggc agaactggca ccttatccag cccaccgagc aggaactcca
      961 gcggctgggc gaacccgtgg tccacctgca tacagtaaga aacggcaaga tcgatcccgc
     1021 tcttcatgac agcatgcgca caggcctccg ccagctccac aggcacgggc agctctcctg
     1081 ccgcactgcc gaaatcacca attgccgtcg ctccaacgcc taaacagaac ggtggcatca
     1141 cgtcatagaa aaagccgttg tagtgatctg gcgcaaacag caccaccagt tcaggggaga
     1201 aagccgcaat acgctcgcgg gcgctggcaa tcacgccatt gacctcatcg agtacctctt
     1261 gcgccgggtc gacatacccc accaacggcg agtgggaaag acagtgaaga taagcgtgca
     1321 tatcaggcta ccttttcgac agaaacgtcg gcatcagggc gggtcagcgt catcaccgac
     1381 gccagtttat tcagcgtatt gcccagggtt tgcggaatgg cggtggcggc aacaaagcga
     1441 tccgggcgca tcaccaccag cgaagcattg tgttgcgcga accagctacg cagacgacct
     1501 tgcgtatcgc ccacgcgtag cacgccgtcg tggttatcct gtgcggtatg aatttgcact
     1561 tccggcacca cctgaatgaa gcgtgtgccc aacgcgcgcc actgctggat ttgctcgtcg
     1621 ctcatccccc acagtggatt gcatccccag ccaattaccg cgaagttcgc gccgatcgcg
     1681 ttatcgagca gcgtcacgtc gccgttttcc agcgtgactt tcggctgaat aaacatcttg
     1741 ccgaccggag agtgcttcgc ttcgccctca cgcaccagcg caccgccgta atattgcggc
     1801 atcggcttga agcgcatttc gaggaagtag cgttttactg gcggcagata attcaacagc
     1861 caggaaacgc cgtcacgtaa cgtaccctgc cagcgtttcg gcggagccag cacgttgccc
     1921 gccgtcacgg acaggtcaat catcgctttg gcgtgatcgc gacgttcttg ttgataggta
     1981 tcgagcagcg catcgcgggc tttcccctgg ataaccaacg ccagtttcca ggcgaggtta
     2041 aaggcgtcgc gcataccact gttatagccc tgcccctgcc acaccggcat gatgtgcgcg
     2101 gcatcgcccg ccagcagtac gcgatcaata cggaaacgtt gcgccagtcg cgcgttatgg
     2161 gtgtagacac gctggcgaat caattcaaca ttgtccggat taggcagcac tttgcttaac
     2221 agcttgcgca tattttgcgg ctcacgcagc tgctcttcgg tttctcccgg catcaccata
     2281 aattcaaagc gacgtaccgc atgaggcagc gcggcagaaa catacgggcg caccggatcg
     2341 caacacaaat agatatgcgg cgtacttaac ggatcgttgg cgatatctac cacaatccac
     2401 tgatttggcg cagttttacc ttcaaacggc acattcaggg tgcgacgaac aaaacttgcc
     2461 ccaccatcac aggccaccag ccactgggct ttgatcgttt cccgctgccc ttctgccgtt
     2521 ttcaggtgca aggtcacttc gtcatcttgc tggctgaagg cctccagctc gcgggaaaac
     2581 aagcagcgca cattcggaaa acgcgacaac ccttccagca tcaccgcatc gacctgtggc
     2641 tgaataaagg cgttacggcg cggccagcca aattcatcgg tcattggctg aatatcagca
     2701 aaacagcggc cttttggggt gagaaaacgc atcgcgtgcc acggtgtagt gtgcggcaga
     2761 acattctcga ccaggccgac cgactgcatg gtgcgcagcg cctcgtcatc aataccaatc
     2821 gcacgcgggt agtcgatcaa cttatcgagt ttctccacca ccagcacgtc aatgcccatc
     2881 tgaccgagat agttcgccat catcagccca accggaccgg caccagcgat cgccacctga
     2941 acgctatggt taacagcagg ctggatgtca gggtgttgta ttgccatttc tgcacaacgt
     3001 tgttttaact atagaaatgt caattaatat gcagaacaat gagcagacgg aatacaaaac
     3061 cgtgcgcggc ttaacccgcg gtctaatgtt attaaatatg ttaaataaac ttgatggcgg
     3121 tgccagcgtc gggctgctgg cggaactcag cggcctgcat cgcaccactg tgcggcgact
     3181 gctggagacg ctgcaggaag agggatatgt ccgccgtagc ccctccgatg acagttttcg
     3241 tctgaccatc aaagtgcggc aattaagcga aggatttcgt gacgaacagt ggatttctgc
     3301 actggcggcc ccgctgctgg gcgatctgtt gcgcgaagtg gtatgaccaa cagatgtgtc
     3361 caccctggat gttgatgcaa tggtggtacg cgaaaccacc caccgtttca gccgcttatc
     3421 ctttcaccgg gcaatggtcg ggcgacgttt accgctcctg aaaaccgcct cgggcctgac
     3481 ctggctggcc ttttgcccgg aacaagaacg caaggaatta atcgaaatgt tagccgcccg
     3541 ccccggtgat gactatcaac tggcacgaga accgttaaag ctgcaagcca ttctggcgcg
     3601 cgcgcgcaaa gagggttacg gacagaacta ccgcggctgg gatcaggagg agaagatcgc
     3661 ctctatcgcc gtaccgctgc gcagtgaaca acgggtgatt ggctgtctga atctggtgta
     3721 tatggcgagc gcaatgacca ttgaacaggc agcggaaaag catcttccgg cgctacaacg
     3781 ggtagcaaaa cagatcgaag aaggggttga atcgcaggct attctggtgg ccggacggcg
     3841 aagcggcgtg catttacgtt gacaccatcg aatggcgcaa aacctttcgt ggtatggcat
     3901 gatagcgccc ggaagagagt caattcaggg tggtgaatgt gaaaccagta acgctatacg
     3961 atgtcgcaga gtatgccggt gtctcttatc agaccgtttc tcgcgtggtg aaccaggcca
     4021 gccacgtttc tgcgaaaacg cgggaaaaag tggaagcggc gatggcggag ctgaattaca
     4081 ttcccaaccg cgtggcacaa caactggcgg gcaaacagtc gttgctgatt ggcgttgcca
     4141 cctccagtct ggccctgcac gcgccgtcgc aaattgtcgc ggcgattaaa tctcgcgccg
     4201 atcaactggg tgccagcgtg gtggtgtcga tggtagaacg aagcggcgtc gaagcctgta
     4261 aagcggcggt gcacaatctt ctcgcgcaac gcgtcagtgg gctgatcatt aactatccgc
     4321 tggatgacca ggatgccatt gctgtggaag ctgcctgcac taatgttccg gcgttatttc
     4381 ttgatgtctc tgaccagaca cccatcaaca gtattatttt ctcccatgaa gacggtacgc
     4441 gactgggcgt ggagcatctg gtcgcattgg gtcaccagca aatcgcgctg ttagcgggcc
     4501 cattaagttc tgtctcggcg cgtctgcgtc tggctggctg gcataaatat ctcactcgca
     4561 atcaaattca gccgatagcg gaacgggaag gcgactggag tgccatgtcc ggttttcaac
     4621 aaaccatgca aatgctgaat gagggcatcg ttcccactgc gatgctggtt gccaacgatc
     4681 agatggcgct gggcgcaatg cgcgccatta ccgagtccgg gctgcgcgtt ggtgcggata
     4741 tctcggtagt gggatacgac gataccgaag acagctcatg ttatatcccg ccgttaacca
     4801 ccatcaaaca ggattttcgc ctgctggggc aaaccagcgt ggaccgcttg ctgcaactct
     4861 ctcagggcca ggcggtgaag ggcaatcagc tgttgcccgt ctcactggtg aaaagaaaaa
     4921 ccaccctggc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
     4981 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg
     5041 agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg
     5101 tgtgaaattg tgagcggata acaatttcac acaggaaaca gctatgatca tgattacgga
     5161 ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa
     5221 tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga
     5281 tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg cgctttgcct ggtttccggc
     5341 accagaagcg gtgccggaaa gctggctgga gtgcgatctt cctgaggccg atactgtcgt
     5401 cgtcccctca aactggcaga tgcacggtta cgatgcgccc atctacacca acgtgaccta
     5461 tcccattacg gtcaatccgc cttttgttcc cgcggagaat ccgacgggtt gttactcgct
     5521 cacatttaat attgatgaaa gctggctaca ggaaggccag acgcgaatta tttttgatgg
     5581 tgttaactcg gcgtttcatc tgtggtgcaa cgggcgctgg gtcggttacg gccaggacag
     5641 ccgtttgccg tctgaatttg acctgagcgc atttttacgc gccggaaaaa accgcctcgc
     5701 ggtgatggtg ctgcgctgga gtgacggtag ttatctggaa gatcaggata tgtggcgaat
     5761 gagcggcatt ttccgtgacg tctcgttgct gcacaaaccg agcacacaaa tcagcgattt
     5821 ccatgttgcc actcacttta atgatgattt cagccgcgcg gtactggagg cagaagttca
     5881 gatgtacggc gagctgcgcg atgagctgcg ggtgacggtt tctttgtggc agggtgaaac
     5941 gcaggtcgcc agcggcaccg cgcctttcgg cggtgaaatt atcgatgagc gtggtggtta
     6001 tgccgatcgc gtcacgctac gtctgaacgt cgaaaacccg gcgctgtgga gcgccgaaat
     6061 cccgaatctc tatcgtgcgg tggttgaact gcacaccgac gacggcacgc tgattgaagc
     6121 agaagcctgc gatgtcggtt tccgcgaggt gcggattgaa aatggtctgc tgctgctgaa
     6181 cggcaagccg ttgctgattc gcggcgttaa ccgtcacgag catcatcctc tgcatggtca
     6241 ggtcatggat gagcagacga tggtgcagga tatcctgctg atgaagcaga acaactttaa
     6301 cgccgtgcgt tgttcgcatt atccgaacca tccgctgtgg tacacgctgt gcgaccgctt
     6361 cggcttgtac gtggtggatg aagccaatat tgaaacccac ggcatggtgc caatgaatcg
     6421 tctgaccgat gatccgcgct ggttacccgc gatgagcgaa cgcgtaacgc gaatggtgca
     6481 gcgcgatcgt aatcacccga gtgtgatcat ctggtcgctg gggaatgagt caggccacgg
     6541 cgctaatcac gacgcactct atcgctggat caaatctgtc gatccatccc gcccggtgca
     6601 gtatgaaggc ggcggagccg acaccacggc caccgatatt atttgcccga tgtacgcgcg
     6661 cgtggatgaa gaccagccct tcccggctgt gccgaaatgg tccatcaaaa aatggctttc
     6721 gctacctgga gagacgcgcc cgctgatcct ttgcgaatac gcccacgcga tgggtaacag
     6781 tcttggcggc ttcgctaaat actggcaggc gtttcgtcag tacccccgtt tacagggcgg
     6841 cttcgtctgg gactgggtgg atcagtcgct gattaaatat gatgaaaatg gcaatccgtg
     6901 gtcggcttac ggcggtgatt ttggcgatac accgaacgat cgccagttct gcatgaacgg
     6961 tctggtcttt gccgaccgca cgccgcatcc agcgctgaca gaagcaaaac accagcagca
     7021 gtttttccag ttccgtttat ccgggcaaac catcgaagtg accagcgaat acctgttccg
     7081 tcatagcgat aacgagcttc tgcactggtc ggtggcgttg gatggcaagc cgctggcaag
     7141 cggtgaaatg cctctggatg ttgctccaca agataaacag ttgattgaac tgcctgaact
     7201 accgcagccg gagagcgccg gacaactctg gcttacggta cacgtagtgc aaccgaacgc
     7261 gaccgcatgg tcagaagccg gacacatcag cgcctggcag cagtggcgtc tggcggaaaa
     7321 cctcagcgtg acactccccg ccgcgcccca cgccatcccg caactgacca ccagcgaaac
     7381 ggatttttgc atcgagctgg gtaataaacg ttggcaattt aaccgccagt caggctttct
     7441 ttcacagatg tggattggcg ctgaaaaaca actgctgacg ccgctgcgcg atcagttcac
     7501 ccgtgcaccg ctggataacg acattggcgt aagtgaagcg acccgcattg acccgaacgc
     7561 ctgggtcgaa cgctggaagg cggcgggcca ttaccaggcc gaagcagcgt tgttgcagtg
     7621 cacggcagat acacttgccg acgcggtgct gattacgacc gctcacgcgt ggcagcatca
     7681 gggaaaaacc ttatttatca gccggaaaac ctaccggatt gatggtagtg gtcaaatggc
     7741 gattaccgtt gatgttgaag tagcgagcga tacaccgcat ccggctcgga ttggcctgac
     7801 ctgccagctg gcgcaggtag cagaacgggt aaactggctc ggattagggc cgcaagaaaa
     7861 ctatcccgac cgccttactg ccgcctgttt tgaccgctgg gatctgccat tgtcagacat
     7921 gtataccccg tacgtcttcc cgagcgaaaa cggtctgcgc tgcgggacgc gcgaattgaa
     7981 ttatggccca caccagtggc gcggcgactt ccagttcaat atcagtcgct acagccaaca
     8041 acaactgatg gaaaccagcc atcgccatct gctgcacgcg gaagaaggca catggctgaa
     8101 tatcgacggt ttccatatgg ggattggtgg cgacgactcc tggagcccgt cagtttcagc
     8161 ggaattccag cttagcgccg gtagctacca ttaccagttg gtctggtgtc aaaaataata
     8221 ataaccgggc aggccatgtc tgcccgtatt tcgcgtaagg aaatccatta tgtactattt
     8281 aaaaaacaca aacttttgga tgttcggttt attctttttc ttttactttt ttatcatggg
     8341 ggcctacttc ccgtttttcc cgatttggct acatgacatc aaccatatca gcaaaagtga
     8401 tacgggtatt atttttgccg ctatttctct gttctcgcta ttattccaac cgctgtttgg
     8461 tctgctttct gacaaactcg ggctgcgcaa atacctgctg tggattatta ccggcatgtt
     8521 agtgatgttt gcgc
//
LOCUS       DADMKF010000158         8515 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.161, whole genome shotgun sequence.
ACCESSION   DADMKF010000158 DADMKF010000000
VERSION     DADMKF010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8515
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.161"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            26..154
                     /locus_tag="JNC24_003411"
     CDS             26..154
                     /locus_tag="JNC24_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020244942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein FimG"
                     /protein_id="HAY9160084.1"
                     /translation="MRDDQDAALKNGDSKTVIVDEITRNAQFPLKARAITVNGNAS"
     gene            258..1172
                     /locus_tag="JNC24_003412"
     CDS             258..1172
                     /locus_tag="JNC24_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY9160085.1"
                     /translation="MGKTISIKVLFGIYLLLMAGKVFAFSCNVDGGSSIGAGTTSVYV
                     NLDPVIQPGQNLVVDLSQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWNN
                     VTYPFPLTTNTNVLDIGDKTPMPLPLKLYITPVGAAGGVVIKAGEVIARIHMYKIATL
                     GSGNPRNFTWNIISNNSVVMPTGGCTVDSRNVTVDLPDFPGSAEIPLGVYCSSEQKLS
                     FYLSGATTDSSRQVFANTAPDATKASGVGVTLMRNGKILATGENVSLGTVNKSKVPLG
                     LSATYGQTGNKVSAGTVQSVIGVTFIYE"
     gene            1506..3785
                     /gene="ydeP"
                     /locus_tag="JNC24_003413"
     CDS             1506..3785
                     /gene="ydeP"
                     /locus_tag="JNC24_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HAY9160086.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRHDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAADRTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFTPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPHS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            4033..4230
                     /gene="safA"
                     /locus_tag="JNC24_003414"
     CDS             4033..4230
                     /gene="safA"
                     /locus_tag="JNC24_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HAY9160087.1"
                     /translation="MYATTVKNKITQRDNYKEIMSVIVVVLLLTLTLIAIFSAIDQLG
                     ISEMGRIARDLTHFIINSLQD"
     gene            4305..5066
                     /gene="ydeO"
                     /locus_tag="JNC24_003415"
     CDS             4305..5066
                     /gene="ydeO"
                     /locus_tag="JNC24_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HAY9160088.1"
                     /translation="MSLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPY
                     ERHFKEIGFTENTIKKYLQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERN
                     HHNFSELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCL
                     YISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYA
                     FRKHFGNSPKRVSKEYRCQSHMGMNTGNTMSALAI"
     gene            5467..7149
                     /locus_tag="JNC24_003416"
     CDS             5467..7149
                     /locus_tag="JNC24_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HAY9160089.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDFTPTEYSTKGKPNIIVLTMDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAI
                     EAAQKSTPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGI
                     PLTETFLPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQ
                     NRGFDYFMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPF
                     MLYLAYNAPHLPNDNPAPDQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQ
                     YDNTIILFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKL
                     ISAMDFYPTALDAADISIPKDLKLDGVSLLPWLQDKKQGAPHKNLTWITSYSHWFDEE
                     NIPFWDNYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTD
                     LQQKDNLAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKALSEAK"
     gene            7201..8358
                     /locus_tag="JNC24_003417"
     CDS             7201..8358
                     /locus_tag="JNC24_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HAY9160090.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIEASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNALQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPLELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCQQCAYKPICNGGCPKHRIT
                     KVNNETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(8324..8446)
                     /locus_tag="JNC24_003418"
     CDS             complement(8324..8446)
                     /locus_tag="JNC24_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9160091.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKESVINYSPFVLQHP"
BASE COUNT         2492 a         1817 c         1835 g         2371 t
ORIGIN      
        1 tactgccgaa aatattcaga tagagctgag ggatgaccag gatgctgcgt taaaaaatgg
       61 cgatagcaaa acggttattg ttgatgagat cactcgtaat gcacagtttc cacttaaggc
      121 aagagctatc acggtgaatg gaaacgcaag ctagggaacg atcgaggcgc taatcaatgt
      181 gatctacacc tggcaataac gtcgattcga ttttcttagc cttcttttta aatcaatttt
      241 taaccttagg atatatcatg ggtaaaacaa tcagtataaa ggtcctgttc ggtatttatc
      301 ttctattaat ggcaggcaaa gtttttgcct tcagttgtaa tgtcgatggc gggtcaagta
      361 ttggagcagg tacaacctct gtttatgtta atctcgaccc tgtaatacag ccgggccaga
      421 atctggttgt agacttgtct cagcatataa gttgctggaa tgattacggc ggctggtacg
      481 acactgatca tataaacctg gtacaaggct cagctttcgc aggctcccta cagtcatata
      541 aaggatcgct gtactggaat aatgtgacct accccttccc gttgacgaca aataccaacg
      601 tattggatat tggtgataag acaccgatgc cattaccgtt aaaactctat attaccccag
      661 ttggggcggc cggtggagtt gtcataaaag cgggggaagt cattgcccgt attcatatgt
      721 ataaaattgc aacattagga agcggaaatc cgcgtaattt tacgtggaat attatttcaa
      781 ataacagtgt tgttatgcca acaggcggct gcaccgttga tagtcgtaat gtcactgtgg
      841 atctcccgga ctttcctgga agcgcagaaa taccccttgg cgtatattgc tcaagcgaac
      901 aaaagctctc attttatctt tcgggtgcca ccactgacag tagccgacaa gtgtttgcta
      961 acacagcacc tgatgcaaca aaagcatcgg gagtgggtgt cactctaatg cgtaatggaa
     1021 aaatactggc tacgggagaa aatgtctccc tggggacagt aaataaaagc aaagtacctc
     1081 tgggtttaag cgcaacctat ggacaaacag gaaacaaggt ttcagccgga accgtccagt
     1141 cagtcattgg tgtcactttt atctatgaat aattgtgatt ttgaccttag tctatgcgct
     1201 ccattcacga aaattgcgca gataattatt ttgtgaaggc tattatccta cacctgtaag
     1261 aaaatccgcg cacaattgaa gccagagtct tattttttat attgaaaaat atgttaattt
     1321 aaacactatg agagatgatg cttaacttat cgtatttaat ctattgttta acgataatag
     1381 attaaaaata gaaagagagt agtcccctac tcttatatat ccatgttggc gataatccct
     1441 ttgtatgtac tgtgcatcat cgctattaca aatcctaata attcatttcc acacaggata
     1501 agtagatgaa gaaaaaaatt gaatcctacc agggtgctgc aggtggttgg ggtgctgtta
     1561 aatccgtagc gaatgcagta cgtaagcaga tggatatacg ccatgatgtt attgccatgt
     1621 ttgacatgaa taagccagag ggctttgact gtccgggttg tgcatggcca gatcctaagc
     1681 acagtgcgtc attcgacatt tgtgaaaacg gcgcaaaagc aatcgcctgg gaagtcacgg
     1741 ataagcaggt aaatgcctct ttctttgctg agaatacggt tcaatcatta cttacctggg
     1801 gagatcacga gcttgaggct gcggggcgtc tcactcagcc tttgaaatat gatgccgtca
     1861 gcgactgtta caagccatta agctggcaac aagctttcga cgaaattggc gcacgccttc
     1921 aaagctatag tgatcccaat caggttgaat tctatacttc gggccgcact tccaatgaag
     1981 ctgcctttct ttatcagctt tttgcccgtg aatacgggag caataacttt cccgactgct
     2041 ccaacatgtg ccatgaaccg acaagcgtgg gtttggcagc gagtatcggt gtaggtaaag
     2101 ggaccgtgtt gctggaagac tttgagaagt gcgatttagt catttgcatt gggcataacc
     2161 ctggtacaaa ccaccctcgc atgctgactt cgttgcgcgc tttagtgaaa cggggagcga
     2221 aaatgatcgc catcaatcct ctacaggaac gtggcctgga gcgatttacc gcaccgcaaa
     2281 acccgtttga aatgctgacg aactctgaga ctcagttggc cagtgcctac tacaacgtgc
     2341 gcattggtgg cgatatggcg ttgctcaagg ggatgatgcg cctgttaatt gagcgcgatg
     2401 atgctgcaag tgccgcaggt cggccctcat tgcttgatga cgaatttatt caaacgcata
     2461 ccgtcggctt tgacgagcta cgccgtgacg ttctcaattc cgagtggaaa gatatcgaac
     2521 gtatttctgg actaagtcag acacaaatcg ccgaactggc tgacgcatat gccgctgccg
     2581 atcgcaccat tatctgttac ggaatgggga tcactcagca cgaacatggt acccagaatg
     2641 tacagcaact ggtcaatctg ctgttgatga aaggtaacat tggcaagcct ggtgcgggta
     2701 tctgcccact acgaggtcac tctaatgtac agggcgaccg aaccgtcggt atcaccgaga
     2761 aaccgtctgc agagtttctg gctcgcctgg gtgagcgcta tggcttcacc ccacctcatg
     2821 cacctggaca tgctgcaatt gccagcatgc aagcaatatg cacggggcag gctcgagcat
     2881 tgatctgcat ggggggcaac tttgcactgg caatgccaga tcgggaagcg agcgctgtac
     2941 cgttaacgca attagatttg gcggtacacg tagccactaa gcttaaccgc tctcatctgc
     3001 tgaccgcacg gcatagctat attctgccgg ttctggggcg tagcgagatt gacatgcaaa
     3061 aaagcggtgc gcaggcggta accgttgagg attcaatgtc gatgattcat gcctcgcgtg
     3121 gcgtgttaaa acccgccggt gtaatgctga aatcagagtg tgcagtggtc gcgggaatcg
     3181 cgcaggcagc actaccccac agcgtggtag cctgggagta tctggtggaa gattatgatc
     3241 gcattcgcaa tgacattgaa gctgtgttac cagagttcgc cgactataac cagcgcatcc
     3301 gacatcctgg aggttttcac ctgataaatg cagctgctga aaggcgctgg atgacgccgt
     3361 caggtaaggc taatttcatt accagcaaag ggctgttaga agatccctct tcagcgttta
     3421 acagtaagct ggtcatggcg acagtacgta gccacgatca gtacaacacg acgatttatg
     3481 gtatggatga tcgctatcga ggggtattcg gtcaacgaga tgtggtcttt atgagtgcta
     3541 aacaagctaa aatttgccgt gtaaaaaacg gcgaaagagt taatcttatt gcgcttacgc
     3601 cagacggtaa gcgcagctca cgccgcatgg atagattaaa agtggtcatt taccctatgg
     3661 ctgaccgctc actggtgacc tattttccag aatcgaatca catgctaact cttgataacc
     3721 acgatccatt aagtggcatt cctggttata aaagtattcc ggttgaatta gaaccatcaa
     3781 attaattcgc ctcctcattt cttctgctgt catccgcaca gcagaagaat ttctcattga
     3841 ctattatcgc gcaatttgct cacattgatt aaattaaacc acacactata agatataaac
     3901 ctctgcctac agctgtaaga aactccgctc agtactgaag tgccagtcct atttcctctt
     3961 ttctccagcc tgttatatta agcatactga ttaacgattt ttaacgttat ccgctaaata
     4021 aacatatttg aaatgtatgc gaccacagtg aaaaacaaaa tcacgcaaag agacaactat
     4081 aaagaaatca tgtctgtaat tgtggttgtc ttattactga cccttacgtt gatagccatt
     4141 ttttcggcaa ttgatcagct gggtatttca gaaatgggtc gcattgcaag agatcttaca
     4201 catttcatta tcaatagttt gcaagactga aaataaacag caaattataa atatgaaatg
     4261 ttaaaaaagt atcgataaaa actttattgt tttaaggaga taaaatgtcg ctcgtttgtt
     4321 ctgttatatt tattcatcat gccttcaacg ctaacatttt agataaagat tacgccttct
     4381 ctgacggcga gatcctgatg gtagataacg ctgttcgtac gcattttgaa ccttatgagc
     4441 ggcattttaa agagatcgga tttactgaaa ataccattaa aaaatatcta caatgcacta
     4501 acatccagac agtgacggtg cctgttcctg cgaagttttt acgtgcttca aatgtaccga
     4561 ctggattgct taatgaaatg attgcttatc ttaactcgga agaacgcaat catcataatt
     4621 tttcagaact tttgcttttt tcttgcctgt ctatttttgc cgcatgcaaa ggtttcatta
     4681 cactattaac taacggtgtg ctatccgttt ccggaaaagt gagaaatatt gtcaacatga
     4741 agctggcgca cccatggaag ctgaaagata tttgtgactg cctgtacatc agtgaaagcc
     4801 tgttgaagaa aaaactaaag caagagcaaa cgacattctc acagattcta ttagatgcca
     4861 gaatgcagca cgcaaaaaat ttgatacgcg tagaaggttc agtcaataaa attgccgaac
     4921 aatgtggtta tgccagtaca tcttatttta tttatgcgtt ccgcaaacat ttcggcaaca
     4981 gtccgaagag agtttctaag gagtaccgtt gtcaaagtca catgggtatg aatacgggca
     5041 acacgatgag tgctttagct atttgattat ttgctaacga gtagtcaacc acgcacgctg
     5101 cgtaagaatt aaatggggca gccattccct gccccgcgtt gtttttaggc gatatattta
     5161 ttgaaataaa taagtgacat ccatcacata tttatgcact tgcataacct gttgcagatt
     5221 atttatgatc tcaattcggc attttgtcag taaaatgcaa taatttatta aatatcaata
     5281 aattagttgt ttatcggcga gaaattactt aatagaacag aaagtaatgt caacgcttta
     5341 tggactgtta tttccctttt tttagctaaa tctgctatct ctttatgtga ctaacttcac
     5401 ttacatccac ttatttctct tcgtaaaatt acttcggaat taagtacaat aagaagagga
     5461 acatttatga agtctgcatt aaagaaaagt gtcgtaagta cctcgatatc tttgatactg
     5521 gcatctggta tggctgcatt tgctgctcat gcggcagatg atgtaaagct gaaagcaacc
     5581 aaaacaaacg ttgctttctc agattttacg ccgactgaat acagtaccaa aggaaagcca
     5641 aatattatcg tactaaccat ggatgatctt ggttatggac aacttccttt tgataaggga
     5701 tcttttgacc caaaaacaat ggaaaatcgt gaagttgtcg atacctacaa aatagggata
     5761 gataaagcca ttgaagctgc acaaaaatca acgccgacgc tcctttcatt aatggatgaa
     5821 ggcgtacgtt ttactaacgg ctatgtggca cacggtgttt ccggcccctc ccgtgccgca
     5881 ataatgaccg gtcgagctcc cgcccgcttt ggtgtctatt ccaataccga tgctcaggat
     5941 ggtattccgc taacagaaac tttcttgcct gaattattcc agaatcatgg ttattacact
     6001 gcagcagtag gtaaatggca cttgtcaaaa atcagtaatg tgccggtacc ggaagataaa
     6061 caaacgcgtg actatcatga caacttcacc acattttctg cggaagaatg gcaacctcaa
     6121 aaccgtggct ttgattactt tatgggattc cacgctgcag gaacggcata ttacaactcc
     6181 ccttcactgt tcaaaaatcg tgaacgtgtc cccgcaaaag gttatatcag cgatcagtta
     6241 accgatgagg caattggcgt tgttgatcgt gccaaaacac ttgaccagcc ttttatgctt
     6301 tacctggctt ataatgctcc gcacctacca aatgataatc ctgcaccgga tcaatatcag
     6361 aagcaattta ataccggtag tcaaacagca gataactact acgcttccgt ttattctgtt
     6421 gatcagggtg taaaacgcat tctcgaacaa ctgaagaaaa acggacagta tgacaataca
     6481 attattctct ttacctccga taatggtgcg gttatcgatg gtcctctgcc gctgaacggg
     6541 gcgcaaaaag gctataagag tcagacctat cctggcggta ctcacacccc aatgtttatg
     6601 tggtggaaag gaaaacttca acccggtaat tatgacaagc tgatttccgc aatggatttc
     6661 tacccaacag ctcttgatgc agccgatatc agcattccaa aagaccttaa gctggatggt
     6721 gtttccttgc tgccctggtt gcaagataag aaacaaggcg cgccacataa aaatctgacc
     6781 tggataacct cttattctca ctggtttgat gaggaaaata ttccattctg ggataattac
     6841 cacaaatttg ttcgccatca gtcagacgat tacccgcata accccaacac tgaggactta
     6901 agccaattct cttatacggt gagaaataac gattattcgc ttgtctatac agtagaaaac
     6961 aatcagttag gtctctacaa actgacggat ctacagcaaa aagataacct tgccgccgcc
     7021 aatccgcagg tcgttaaaga gatgcaaggc gtggtaagag agtttatcga cagcagccag
     7081 ccaccgctta gcgaggtaaa tcaggagaag tttaacaata tcaagaaagc actaagcgaa
     7141 gcgaaataac taaaccttca tgcggcggat ttttccgccg ccttattgag cgagatagcg
     7201 atgcacgtta cagccaagcc ctccagtttt caatgtaatc tcaaatgtga ttactgtttt
     7261 taccttgaaa aagagtcgca gtttactcat gaaaaatgga tggatgacag cactctgaaa
     7321 gagttcatca aacaatatat cgaagcgtct ggcaatcagg tctattttac ctggcaaggc
     7381 ggtgaaccca ctctggctgg cctggatttt ttccgtaaag ttattcacta tcaacaacgc
     7441 tatgcaggcc aaaaacgtat ttttaatgca ttacaaacga atggcatttt attgaataat
     7501 gaatggtgtg cctttctcaa agaacatgaa tttctggttg gtatctcgat cgatggcccc
     7561 ctggagttac atgaccgtta cagacgcagt aattcaggta acggtacttt tgcaaaagtg
     7621 atagcagcca tcgagcgtct gaaatcatat caagtagagt ttaatacgtt aaccgtcatt
     7681 aataacgtta atgtccatta ccctcttgag gtttatcatt ttttaaaatc tatcggcagt
     7741 aaacatatgc aatttatcga attgctagaa accgggacgc cgaatattga tttcagtggt
     7801 catagtgaga acacattccg tatcattgat ttttctgtgc ctcccacggc ttatggcaag
     7861 tttatgtcaa ccatttttat gcaatgggtt aaaaacgatg tgggtgaaat tttcattcgt
     7921 cagtttgaaa gctttgtcag ccgttttttg gggaatgggc atacaagttg tattttccag
     7981 gagtcctgca aggataatct ggttgttgaa agtaatggag acatttacga atgcgaccat
     8041 tttgtctatc cacagtacaa aattggaaac attaataaat ctgaactcaa aacgatgaac
     8101 agtgtacaac tgacagcgca aaaaaaacgg atttcagcga aatgtcagca atgtgcatat
     8161 aaacctatct gcaatggcgg ttgtcctaag catcgtatta ctaaagtaaa caatgagact
     8221 gtttcttatt tttgcgaagg ttataaaatc cttttttcaa ccatggtacc ttatatgaac
     8281 gccatggttg agttagctaa gaacagagta ccgctttacc acattatgga tgttgcaaaa
     8341 caaatggaga ataattaata acgctctctt ttaactccat tcttagcctc cttcttatga
     8401 ccttaacaag gcacccgaca taaattcctg tcgggtgttt cgtcatcatc atcttccctt
     8461 gcgaaaatcc tggcttacgt ggtatgcgtc agggcattag caataccctt gatta
//
LOCUS       DADMKF010000159         8460 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.162, whole genome shotgun sequence.
ACCESSION   DADMKF010000159 DADMKF010000000
VERSION     DADMKF010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8460
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.162"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            120..782
                     /locus_tag="JNC24_003419"
     CDS             120..782
                     /locus_tag="JNC24_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HAY9160092.1"
                     /translation="MSIEHILIGKHHGGSDYWRTPLLLFKNLHREFGFTLDGAATEHD
                     TLLPRFTNDISRQSWVSEKVFCNLPYSDIPSFLLKASEADLVAFLIPHRANTSYWLRH
                     IYSNNHCHEIRILHRAVKYLPPAGHNGLTIRSPFPSAVVVFKKEPRKHEITQTVCCGD
                     TLLPLTIINRGGLRGRPTIYPPETLDSFIKLYRQGKPIKCIADALRMPLSTSYRIAQR
                     LS"
     gene            1165..2118
                     /locus_tag="JNC24_003420"
     CDS             1165..2118
                     /locus_tag="JNC24_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAY9160093.1"
                     /translation="MEKFHMFDDRIYQMTNGVKAADRIIYTVMLNEQKFYHDKGQYYT
                     PSYAHLGQYAGITRTSVIACMKQLEAHGLIKKTNVASNQCVIKVYSLDEVPEVLTELT
                     AKEARKAKLESKVTQSVKKDDPLSQNSLPTESKFFTQSVKIFDTYNNSNNKKELINNN
                     KEDDFEEEYFKKGASLCDSHECDSLGIEEAIKETVKTSDLDDLDSEAAKEVVKKEDKI
                     SDVIASSDHKNTQCPATDDELPDVDLDDEEDPEKELKREQRKAEIKERFRNAKLKNKV
                     SDLDDFDDSDDDFTFTPKKKTHTEPKKEKVKVRRVYGGGMF"
     gene            2118..2414
                     /locus_tag="JNC24_003421"
     CDS             2118..2414
                     /locus_tag="JNC24_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000860811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9160094.1"
                     /translation="MKTYNGERKYRDYLLRRYSISREGYLMKDTHGEVYRIRPKKEGR
                     DYYFVDDVTDLKIDALKFAVLYHYDLWDSVHQLRLKDGDPGNLRATNIIKGKCR"
     gene            2738..3028
                     /locus_tag="JNC24_003422"
     CDS             2738..3028
                     /locus_tag="JNC24_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000149936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9160095.1"
                     /translation="MTHTVTRLLPEAFERLAAIKAKYPDMKHTALVGEILKESVNLSP
                     FDNVKGVAEGSKAIRITTGQLELLKAQSAVAGCSMIQVLSAKVHQLADRLEV"
     gene            3249..3464
                     /locus_tag="JNC24_003423"
     CDS             3249..3464
                     /locus_tag="JNC24_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9160096.1"
                     /translation="MKTITVQLQTNKAFRYFENLLELYEGWGSIHGKDDIYLHLSAPN
                     YSLKTPVKQSWLKDYGHQMGLLVSDLS"
     gene            3611..3916
                     /locus_tag="JNC24_003424"
     CDS             3611..3916
                     /locus_tag="JNC24_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9160097.1"
                     /translation="MNYPMQIMVDQETMNWLIDLNRSGFNIYFMHCFEASTYIDKHII
                     NEIKLGDYVHKLEVTDPYNKRFIIEECNRLDLSPEQLLKLNVFLTYQNELNPYSEVM"
     gene            4392..5102
                     /locus_tag="JNC24_003425"
     CDS             4392..5102
                     /locus_tag="JNC24_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY9160098.1"
                     /translation="MTNDIYINGKVIDSFSDWSPQTEHPAITIQRKEHDKRIELEQSL
                     KMSPKTTIFVSPEPQEMPDICKSEALLELEKKYYQLLKEQRLRLDDAYSKVTLMQSAI
                     EPSEFEIQDELSQKPFVYYQYEDNDGFGTFQENVAAVISSLPAGHRIVKMVKASRGAG
                     QFIYMTNKSDEELAEIARQNILASRNKQLDNVKLYLSRELQAMKELVKEYEDAKKIAM
                     QADIEQLTKISSKYAKAI"
     gene            5127..5333
                     /locus_tag="JNC24_003426"
     CDS             5127..5333
                     /locus_tag="JNC24_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9160099.1"
                     /translation="MIDLTERYELIKAISERLKEQPNKPKRIKSFTSSKQKNRIRKIA
                     KWCLDKLPSDCQIVGEDGNYYLVR"
     gene            5432..7612
                     /locus_tag="JNC24_003427"
     CDS             5432..7612
                     /locus_tag="JNC24_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HAY9160100.1"
                     /translation="MSKFTVDSFIVELSFSENVIKGLQRVEKAALQSAQRIERNLNRA
                     FKVDTKQLDSNLTSSLGQLERKFNKTFDKIEQRARSTKAFQFATRFNDTVNPPKQSRQ
                     PRISGNRAITAAHSVNMSKLGDINPLMAKMFKAQYYSLSGKAGNIGSEKFNAELAKLN
                     QSLRETLNKLRSQTKTASINNTSDAFNNLASNAIKLSGAFYSVMGALNAYKAIMNAGL
                     KRDSAQRAAKFVLGDKASEAEVFIRNLADKTGLNISEGLSSYAKFAAGAQGSMSQEQT
                     QELFGNATAMSRLMGLSNDELNGILRAFEQMASKGKIQAEELRGQLGDRMAGAFKLFA
                     EALGMTATELDAAMKNGKVLSADTLPKVAKQMGLMIDKAGGWAEVTKSTQTALGKLAN
                     NWDDTMVKIFSGSQDELNGFLSSLSNLLSEMGMSSSIAGDAIGGLIDMLKAGVDDIRV
                     FNNTIEGWILQTKKFYYSLDDTKRKLLDEVGDGFINFVKGLAIALSAKTLFSATTGVM
                     NLTRAITTLGTRANQVAGQVATGKGGGKLKGVAGGVGSALAIGYADDGYETALALASM
                     IPQIRGVTLGLYALKKALDFMNQEVVKNANMHPSGVGVGSDFNPVYSGDPNKPNMINE
                     GWSRIFSSMGELFNNATMNIARFNHPELNNIKQDSALTSADIQGLRDEISALSKRIQE
                     PVKVSLGGQVVVRPDEPSFFTFNQSANDQFTQDMLLSSSYPEEE"
     gene            7769..8460
                     /locus_tag="JNC24_003428"
                     /pseudo
     CDS             7769..8460
                     /locus_tag="JNC24_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000640578.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         2774 a         1521 c         1801 g         2364 t
ORIGIN      
        1 taagataaga gatagatatt ccagaaagga tatccgttta gaaagcctgt taagtatctc
       61 ttatcttaaa agagagtaaa ccttgtcaag agtgattgca ataaaatttt ttatttctta
      121 tgtcaattga gcatatttta atcgggaaac atcatggggg ttctgattac tggagaactc
      181 cattgttgtt atttaagaac ttacaccgtg aatttggttt tacgctagat ggtgctgcaa
      241 ctgagcatga cacgctgtta ccacgattca ccaatgatat aagccgtcaa tcatgggtta
      301 gtgaaaaggt cttctgtaac ctgccgtaca gcgatattcc gtcgttcctc ttaaaagcgt
      361 ctgaagcgga tttagtagct ttcctgatac cgcatagagc caacaccagc tactggctta
      421 ggcacattta cagcaataac cactgtcatg aaatccgtat tcttcatcgt gcagttaaat
      481 accttccacc agcaggccat aacgggctta ctattcgttc accgtttcct agtgcagtcg
      541 ttgtttttaa aaaagaacca cgtaagcacg aaattacaca gacggtttgc tgtggtgaca
      601 cgctattacc gttaaccatc attaacagag gaggcttgag aggaagaccc accatttacc
      661 cacctgagac gctcgacagc ttcataaaac tatacaggca aggaaaaccc attaagtgca
      721 ttgcagatgc tctcagaatg cctctgagca cgtcatacag gattgctcaa aggttgagct
      781 aaaattctca taaatatggt catttacaag ataggtgatt cagatattaa gaagtatccc
      841 tgtgaacaat aaatgaccaa tcagtaatta ttctgtgaaa taattgttgc aatttataat
      901 cacatctgtt atagttttta tatggggtga taaaagaact cagaacatta agaggttaca
      961 acgttgggag gcgtcagggt attgacagat tttcacggcc tcttattcaa tcaaggcttt
     1021 tccttttgct gttaatactc gcgtaatcgc tcaggcttct gagttccttt atcagtccat
     1081 cttctaatta attcactccc tctggggttg gtcactatac gtggtcagcc ctttctttgt
     1141 tttattttta aaaaggaaaa tataatggaa aaattccaca tgtttgatga ccgtatttat
     1201 cagatgacta atggcgttaa agctgccgac cgtattattt acactgtcat gttgaatgaa
     1261 caaaagtttt atcacgataa ggggcaatat tatacccctt catatgctca tcttggtcag
     1321 tatgctggaa tcacaagaac atccgtgatt gcatgtatga aacaacttga ggcacatggt
     1381 ttaatcaaga aaactaacgt tgcaagcaat cagtgtgtta ttaaagttta ttcacttgat
     1441 gaagtaccgg aggttttaac agagcttaca gctaaggaag ccagaaaggc aaagctggag
     1501 tctaaagtta ctcagtcagt caagaaagat gacccactga gtcaaaattc tttacccact
     1561 gagtcaaaat tttttactca gtcagtcaaa atctttgaca cgtataataa cagtaataac
     1621 aagaaagaac ttattaataa caataaagaa gatgactttg aagaagaata ttttaagaag
     1681 ggagcttcgc tctgtgattc acatgaatgt gattctcttg gtattgaaga ggctattaaa
     1741 gagactgtta aaacatctga cttagatgac cttgatagtg aagctgctaa agaggtcgtt
     1801 aaaaaggaag ataaaatctc tgatgtgatt gctagtagtg accataaaaa cacacagtgc
     1861 cccgccactg atgatgaatt accggatgtt gaccttgatg atgaagaaga ccctgaaaag
     1921 gaattgaaac gtgaacagcg taaagcagaa attaaagaaa gattcagaaa tgcgaagcta
     1981 aagaacaagg ttagtgacct cgatgacttt gatgatagtg acgatgattt tacttttaca
     2041 ccaaaaaaga aaactcacac cgagccaaag aaagaaaaag ttaaagtcag acgtgtttat
     2101 ggtggaggaa tgttttaatg aaaacttata atggcgagag aaaataccgt gactatttac
     2161 tcagacgtta cagcatttct cgtgaaggtt acttgatgaa agatactcac ggggaagttt
     2221 accggattcg accgaagaaa gaagggaggg attattattt cgtcgatgat gtgacagatt
     2281 taaagattga cgctttgaag tttgcggtgc tgtatcatta cgatttatgg gattcagtcc
     2341 accagctacg attaaaagac ggtgaccccg gtaatctgag ggccaccaat attatcaagg
     2401 gaaagtgccg gtaaggccac cagctattta tttactgaaa tctctatctc aacacgaaga
     2461 agtttaagtc gaattgtaag ctttgtcttt tttgtaatga ctcgattcat aacgcaaaac
     2521 accaatagca caataaatac cgcaaagccc aacgagctaa aacgatgaga ttcaataatt
     2581 gctgtgaaaa aatcatgcat taattttcct tttgtgaaag gaaacagcac ctgtatcagc
     2641 ccctacaacc atagtatcat gtaaatgagg tactgacaat atgttaaata gatgttaaga
     2701 ttgtagtatg agtaagagat ttaaggagat ttttacaatg actcacacag ttacgcgcct
     2761 tttgccagag gcatttgaac gactggcagc aatcaaagcc aaatatcccg atatgaagca
     2821 cacagcgctt gttggggaga ttttaaaaga gtcggtaaac cttagccctt tcgataacgt
     2881 taagggcgtc gcagagggtt ctaaggccat cagaatcaca acgggccaac tggaactact
     2941 caaagcacag tccgcagtag caggttgctc aatgattcag gtgttaagcg ctaaggtcca
     3001 tcaactggcg gatagattgg aggtttaatg tgttataggt tccaatattc cacacttggc
     3061 cgccacctaa tcagacagaa tttagcagat gctgaatttg aagatgtgac cctcaaagca
     3121 aacaaacgat taaaaccctc tcaaatccac cagcttaaac acgaattaaa gccactggta
     3181 atactcgact ataacgaccc tgaacagctt gagcagggaa ttaagcttat ttctgaatgg
     3241 ggtgcttgat gaaaactatc acagtacaac ttcagactaa taaagcattt cgttattttg
     3301 aaaacttgct tgagttatac gaaggatggg gaagtatcca cggcaaagat gatatctatc
     3361 ttcacttgtc agcccctaac tattctctga agactccggt taagcaaagc tggttaaaag
     3421 actacggtca ccagatgggg ctattagtct cagacttgag ctaaaaacag caaaacatat
     3481 ctctcaggct ttaccagacg caccagaaac gtttttaagc ccctaatcag ggtaacttag
     3541 ggaatcgcct taagtggtct aaaaaagggg ttaaaacgtc ttagaatgcg atacaggaag
     3601 gattttaaaa atgaattacc caatgcaaat tatggttgac caagaaacca tgaattggtt
     3661 gattgacctg aacaggtcgg gctttaatat ctatttcatg cattgttttg aagcgtcaac
     3721 gtatattgac aagcatataa tcaacgaaat taaacttggt gactatgttc acaagctgga
     3781 agtaacagac ccatacaata agcgctttat cattgaagag tgcaatcgac ttgatttaag
     3841 ccctgagcaa ttattgaagc tgaatgtatt cttgacctat cagaatgaat taaaccctta
     3901 cagcgaggtg atgtaatgac actcgttatt ctctggatga ctttagggtt atttgttggt
     3961 tgctgggtag gtaatacggt aaataagaat tgatgttacc aatctgttaa aaagctgttg
     4021 acaaataacc ttaagcatga gatgatggta atagggaaga gcaactcttc ctgaattaaa
     4081 ctattaatca ttctacatca atgatagtta ttctgaaagg tgcaatgctt ttcgctacat
     4141 gacctttcag gttagtctga gaggtcatct cgaaaagtac tcttgttcat tcgtttgaat
     4201 cctttttgaa tcctttagat gttttaattt cttcacctta tgaatggccc tttataagaa
     4261 attatctagg gctattcagc ttttaaaaag catacttggc aaaccgcctt ttaagtctct
     4321 tctaagttta gataggctgt caggtgtgct ttttaaaaac tgaaacactt ccgcttagaa
     4381 gaggaaaagc aatgacaaat gatatttaca ttaatggcaa agtgattgat agcttcagtg
     4441 actggtcgcc acagactgaa catccagcta tcactattca gcgtaaagag catgacaagc
     4501 gtattgagct tgagcaatcg ctaaagatgt caccaaaaac aaccatcttt gtgtctcctg
     4561 agcctcaaga gatgcctgat atttgcaaga gtgaagcatt acttgagctt gaaaagaaat
     4621 attaccaact gctgaaagaa cagcgcctta gattagatga cgcttacagc aaagttacct
     4681 tgatgcaatc agctattgag ccgtcagaat ttgagattca ggatgaattg tcacagaaac
     4741 catttgtcta ttaccaatac gaagataatg atggttttgg tacttttcaa gaaaacgtcg
     4801 cagccgtgat tagtagtctt cctgctggac atcgaattgt gaagatggtt aaggcatcaa
     4861 gaggtgctgg tcagtttatt tacatgacta ataagagcga tgaagaactc gctgaaattg
     4921 ctagacagaa catcttggca tcacgtaaca agcaacttga taatgttaag ttgtatctct
     4981 caagagagct tcaggctatg aaagagcttg tgaaagaata tgaagatgct aagaagattg
     5041 ctatgcaggc tgatattgag cagcttacga agatttctag caaatatgca aaggctattt
     5101 aattaataat taaaaggtgg tggtgaatga ttgatttaac agaacgctat gagcttatta
     5161 aagcaattag tgaacgattg aaagaacaac ccaacaaacc aaaacgaatc aaatcattca
     5221 cctcctctaa gcaaaagaat agaatcagga aaatagctaa gtggtgtctt gataaattac
     5281 caagtgattg tcagattgtt ggtgaagatg gtaattatta tctggttagg taacaagatt
     5341 ggttgggtga aaatctacgg gagagattat cgagtgatat cggtagtctt tccctttttc
     5401 gtatatgaac acttaaagag gtgaaacatg aatgagcaaa ttcacagttg atagttttat
     5461 cgttgaactt agtttcagtg aaaacgttat taagggcttg caaagagttg aaaaggcagc
     5521 tttgcagtca gcacaaagaa ttgaacgcaa tttgaataga gcgtttaagg tcgataccaa
     5581 gcaacttgac agcaatctga caagttcttt agggcagctt gaaaggaagt ttaataagac
     5641 ctttgacaag attgagcaga gagcaagaag cacaaaagca tttcagtttg ccaccagatt
     5701 taatgacact gttaatcctc caaaacaatc aaggcaacca cgaattagcg gaaacagagc
     5761 aatcacagca gcgcatagcg tgaatatgtc taagcttggt gatattaatc cgctgatggc
     5821 taaaatgttt aaagcacagt attacagctt aagcggtaag gctggtaata ttggaagtga
     5881 aaagtttaac gctgaacttg caaaattaaa tcaatctctt cgtgaaacgt tgaataaact
     5941 caggtcacag acaaagaccg ccagcattaa caatacaagc gacgcattta ataatcttgc
     6001 aagcaatgcg attaaattat ctggtgcatt ttactctgtg atgggtgctc ttaacgctta
     6061 caaggccatt atgaatgcag gtcttaaacg agattccgca cagagagccg ctaagtttgt
     6121 acttggtgat aaagcatctg aagctgaggt atttattcga aatttagcag acaagacagg
     6181 tcttaatatc tctgaagggc tatcaagcta cgctaagttt gcagcaggtg ctcagggttc
     6241 tatgtcacag gaacagacac aggagctatt tggtaatgca acggctatga gtcgcttaat
     6301 ggggctttct aacgatgagc taaacggcat tttgagagca tttgagcaga tggcgagcaa
     6361 gggcaagatt caggctgagg aattacgtgg gcagttaggt gaccgtatgg ctggtgcatt
     6421 taagctattc gctgaagcat tgggtatgac tgctacagag cttgatgcag ccatgaaaaa
     6481 cgggaaggta ctttccgctg atactctccc taaagtcgct aaacagatgg gcttgatgat
     6541 tgataaagcg ggtggctggg ctgaagtcac taagagcact cagacagctt taggtaagtt
     6601 agcaaacaat tgggatgata cgatggtgaa aatcttctca ggttcacagg atgagctaaa
     6661 cggcttctta tccagcctga gtaacctgct tagtgaaatg gggatgagtt cttctatcgc
     6721 tggtgatgct atcggtggac ttatcgacat gcttaaagct ggtgtagatg atattcgcgt
     6781 attcaataac accattgaag gctggatttt acagactaag aagttctatt acagccttga
     6841 tgacactaaa cgaaaactac ttgatgaagt cggtgatggt tttatcaact tcgtcaaagg
     6901 tcttgctata gcgttatctg caaagacgct attcagtgct actacagggg ttatgaacct
     6961 gacaagagca atcacaacgt taggtacaag agctaatcag gtggctggtc aggtagctac
     7021 tggtaaaggt ggtggaaaac tgaaaggtgt agctggtggt gtaggtagtg ctcttgcaat
     7081 tggttatgct gatgacggtt atgaaacagc gttagcatta gcttcaatga ttccacaaat
     7141 cagaggcgtc actcttggtt tatatgcatt gaagaaagca ctggatttta tgaatcagga
     7201 agtagtcaaa aacgctaata tgcatccgtc tggggtaggt gtgggtagtg actttaatcc
     7261 cgtgtattct ggtgacccta ataaaccgaa tatgattaac gaaggatgga gtagaatttt
     7321 tagttcaatg ggtgaattgt ttaacaatgc cacaatgaat attgcaagat tcaatcatcc
     7381 agagttaaac aacattaagc aagattcagc attaacgagt gcagatattc agggtctgag
     7441 ggatgaaatt agtgctttga gtaagcgtat tcaggagcca gtgaaagttt cacttggtgg
     7501 tcaagttgtc gttcgaccag atgaaccatc tttctttact ttcaatcaat cagctaatga
     7561 ccagttcacg caggatatgc tcttaagttc aagctatcca gaggaagaat gatttaacgg
     7621 tgttctacgt gaataaagca aagttaatac ccacaccaaa aattattttt cacgtagctc
     7681 acgcttaaca gagaaaatac atatatatca aaggcttggt taaaaatgat taaaatacac
     7741 taagcctttg aaatcaaagg agaatttaat gatttacact aatcagcttc gttacagtgg
     7801 caaagatggc atctatttcc acttaagaga caatgtagat gccttcttaa ccttatcagc
     7861 aactgaaaac atggaatttg atagccctat gcaggtgact acacagaaca tgcaattatg
     7921 aaggaagctt attattaact cgtcagggtc aattagtaga aaacttcatt gacactctgg
     7981 aaaactggcg tgaccagaag cagattattt ctgttatctg taaagatgga attaaaattg
     8041 acgattccat tattaccaac tttaaagcct ctaaagatgt tactatctct aacggtcttc
     8101 gtatccagct aacttttcag gaaattaact ttaaagcaat cgtaggtcaa actgatattt
     8161 cagcagccac tggcaaaacg gctaccacaa atgacggtgg tgtgaccagt aagaagaatg
     8221 caggtaacgt gaccactgaa cgcaataacg cgcctatgct atcgtgcaaa gagttaatga
     8281 atattagcga tgtatccggt tacgctccag ctatcagaaa tgatattctc gatgctcaga
     8341 ttaaatgtaa taaggcttgg aagctggact attcagactt aacagaagct gaaaaaacca
     8401 acgtcaccaa tgctaatagt aacgctggtc gagtagcttc aaagcttaaa aagtactcag
//
LOCUS       DADMKF010000160         8434 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.163, whole genome shotgun sequence.
ACCESSION   DADMKF010000160 DADMKF010000000
VERSION     DADMKF010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8434
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.163"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(185..261)
                     /locus_tag="JNC24_003429"
     tRNA            complement(185..261)
                     /locus_tag="JNC24_003429"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(225..227),aa:Asp,seq:gtc)
     gene            complement(394..1125)
                     /gene="dnaQ"
                     /locus_tag="JNC24_003430"
     CDS             complement(394..1125)
                     /gene="dnaQ"
                     /locus_tag="JNC24_003430"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HAY9160101.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVEKKGGSCLWRA"
     gene            1190..1657
                     /gene="rnhA"
                     /locus_tag="JNC24_003431"
     CDS             1190..1657
                     /gene="rnhA"
                     /locus_tag="JNC24_003431"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HAY9160102.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            complement(1654..2376)
                     /locus_tag="JNC24_003432"
     CDS             complement(1654..2376)
                     /locus_tag="JNC24_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAY9160103.1"
                     /translation="MKPARVPQTVVAPDRWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDVCLLAHTLPW
                     CTDPHRLLRETDRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            2410..3165
                     /gene="gloB"
                     /locus_tag="JNC24_003433"
     CDS             2410..3165
                     /gene="gloB"
                     /locus_tag="JNC24_003433"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HAY9160104.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAIAANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVIATPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTASQMYQSLNKLSALPDDTL
                     VCCAHEYTLSNMKFALSILPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINVINEETLLQQPEERFAWLRSKKDRF"
     gene            3237..4595
                     /gene="mltD"
                     /locus_tag="JNC24_003434"
     CDS             3237..4595
                     /gene="mltD"
                     /locus_tag="JNC24_003434"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HAY9160105.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAD
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(4644..5267)
                     /locus_tag="JNC24_003435"
     CDS             complement(4644..5267)
                     /locus_tag="JNC24_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAY9160106.1"
                     /translation="MSGLPQGRPTFGAAQNVSAVVAYDLSAHMLDVVAQAAEARQLKN
                     ITTRQGYAASLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRLIVMDVMSP
                     GHPVRDIWLQAVEALRDTSHVRNYASGAWLTLINEANLIVDNLITDKLPLEFSSWVVR
                     MRTPEALVDAIRIYQQSASTEVRTYFSLQTDGSFTSDIIMVEAHKAA"
     gene            complement(5271..6071)
                     /locus_tag="JNC24_003436"
     CDS             complement(5271..6071)
                     /locus_tag="JNC24_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HAY9160107.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            6312..7226
                     /locus_tag="JNC24_003437"
     CDS             6312..7226
                     /locus_tag="JNC24_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY9160108.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDKEIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAPGGAVREA"
     gene            complement(7223..8026)
                     /gene="dkgB"
                     /locus_tag="JNC24_003438"
     CDS             complement(7223..8026)
                     /gene="dkgB"
                     /locus_tag="JNC24_003438"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HAY9160109.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGHAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNATPAQVILAWAMGEGYSV
                     IPSSTKRKNLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
     gene            complement(8189..8265)
                     /locus_tag="JNC24_003439"
     tRNA            complement(8189..8265)
                     /locus_tag="JNC24_003439"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8229..8231),aa:Asp,seq:gtc)
BASE COUNT         2261 a         2018 c         2034 g         2121 t
ORIGIN      
        1 aaatatattt aaattgaatg atttgatagt atgattaaat attaatgata tggtctacca
       61 tatgtgatta acacatttac agcataacaa aaccatagac cgaagaaata gcgggattta
      121 aatgcaaaaa aaccgcaaaa ttaaaaatct tgcggttctc tgaactcatt ttcatgagtg
      181 aatttggcgg aacggacggg actcgaaccc gcgcccccct gcgtgacagg caggtattct
      241 aaccgactga actaccgctc cgcgttgtgt tccgttggga acgaggcgaa tattacggat
      301 tgcctcaccc ttcgtcaacg gtttttctca tcttttgaat cgtttgctgc aaaaatcgcc
      361 caagccgcta tttttagcgc cttttacagg tatttatgcc cgccagaggc agcttccgcc
      421 cttcttctcc accagatcaa gacgggcttc atgagctgca agctcttcat ctgtcgcaaa
      481 aacaacgcgt aacttacttg cctgacgtac aatgcgctga attgttgctt caccttgttg
      541 ctgctgtgtc tctccttcca tcgcaaaagc catcgacgtt tgaccaccgg tcatcgccag
      601 ataaacttcc gcaaggatct gtgcatcgag taatgccccg tgtagcgttc gtttactgtt
      661 atctatttcg tagcgggcac ataacgcatc gaggctgttg cgcttaccag gaaacatttt
      721 cctcgccacc gcaaggctat cggtgacctt acagaaggta ttggtcttcg gaatatcgcg
      781 cttaagcaac gaaaactcgt agtccataaa gccgatatcg aacgctgcgt tatggatcac
      841 caactccgcg ccgcggatat agtccatgaa ctcatcggct acttcggcaa acgtgggctt
      901 atcgagcaaa aattcatcgg caataccatg tacgccaaag gcttccggat ccaccagccg
      961 atcgggtttg agataaacat ggaagttatt gcccgtcagg cgacggttca ccacttcaac
     1021 ggcaccaatc tcaatgatct tgtggccttc atagtgcgca ccaatctggt tcataccggt
     1081 ggtttcggta tcgagaacga tctggcgtgt aattgcagtg ctcatagcgg tcatttatgt
     1141 cagacttatc gttttacagt tcgattcaat tacaggaagt ctaccagaga tgcttaaaca
     1201 ggtagaaatt ttcaccgatg gttcgtgtct gggcaatcca ggacctgggg gttacggcgc
     1261 tattttacgc tatcgcggac gcgagaaaac ctttagcgca ggctacaccc gcaccaccaa
     1321 caaccgtatg gagttgatgg ccgctattgt cgcgctggag gcgttaaaag aacattgcga
     1381 agtcattttg agtaccgaca gccagtatgt ccgccagggt atcacccagt ggatccataa
     1441 ctggaaaaaa cgtggctgga aaaccgcaga caaaaaacca gtaaaaaatg tcgatctctg
     1501 gcaacgtctt gatgctgccc tagggcaaca tcaaatcaaa tgggaatggg ttaaaggcca
     1561 tgccggacac ccggaaaacg aacgctgtga tgaactggct cgtgctgcgg caatgaatcc
     1621 cacactggaa gatacgggct accaagtcga agtttaagct tgtggtttac gacattgccg
     1681 ggtggctcca accgcctggc gaattcgtgg cttgttttta ctctgtttca tcgggtttag
     1741 cgttaaagga atagtccgtt tccgggcaac aataagttgt aagcaaccaa gcgcaggaat
     1801 atgcgcattc aatagttttc ctccgtgttt gttccacggg agaacgtgga aacggctggc
     1861 gtgcagcact tcaaaattca acaaagagag ccagtccagt tgccgcatca gagtaaacat
     1921 ccggctgtta tagggcgagg tttttcgcaa taccggcaca agtttgcgta atcccattaa
     1981 actaatggga ttgaagccac taatcaccag ccagccatca tcaatcaata cccgatcggt
     2041 ttcacgcaat aaacgatgcg gatcggtgca ccacggcaat gtatgtgcca gtagacaaac
     2101 atcaacggat ttatcggcaa aaggaagatg aagtggatcc gcctgcacct ggacgggcat
     2161 tccttgcgca gaaacattca cttgatgaga aaccgcgcac gcttcgcaat tgatttctgc
     2221 gcttaaattg ccaatcttaa gcagatgaaa accatacatt ttagtgaacc acgggttgag
     2281 ctggcgctcc agcgccttgc gataaagctc tccccagggc aaatcgcccc agcgatcagg
     2341 agccacgaca gtttgaggga ctcttgccgg tttcatcaca accttccgtt tcacactaag
     2401 aggtaatcta tgaatcttaa cagtattccc gcctttgatg acaattacat ctgggttttg
     2461 aatgatgaag caggtcgctg cctgattgtc gatcccggag acgcagagcc agtattaaac
     2521 gccattgccg ccaataactg gcaaccggag gccatatttc tcacccacca tcatcacgat
     2581 cacgttggcg gcgtaaaaga actggtggaa aagtttccgc aaattgtggt gtatggtcca
     2641 caagagacac aagataaggg aacaacacag gtagtcaaag atggcgaaac tgccttcgtt
     2701 ttggggcatg aatttagtgt aattgccaca ccgggtcaca ctttaggaca tatctgttac
     2761 ttcagtaaac cttatctatt ttgcggcgac acgctgtttt ctggtgggtg tggtaggttg
     2821 tttgaaggga cagcatcaca aatgtatcaa tcacttaata agttaagtgc gttacctgac
     2881 gatacattgg tatgttgtgc tcatgaatat acgttatcaa atatgaagtt tgctttgagt
     2941 attcttccgc acgatttgtc cataaatgat tattatcgta aagttaagga gttacgggca
     3001 aaaaatcaaa taacactacc cgtaattctg aaaaatgagc ggcaaattaa tgttttttta
     3061 agaacggaag atattgattt aattaatgta attaatgaag aaacattatt gcaacaacct
     3121 gaagagcgtt ttgcatggtt aaggtcaaag aaagataggt tctgataaaa ctttcttgtc
     3181 atcggctccg ttcgccgtta tgatcggtcg tcttttaagc aactattgac acacacatga
     3241 aggcaaaagc gatattactc gcctctgtcc tgctcgtggg ttgccagagt accggcaacg
     3301 ttcaacagca cgcacagagc ctttctgcag ctggtcaagg ggaagcagca aagtttacaa
     3361 gtcaggcacg atggatggac gatgggacgt ctatcgcgcc agatggtgac ttgtgggctt
     3421 tcattggcga cgagctaaag atgggaattc cggaaaatga ccggattcgc gaacagaaac
     3481 agaaatattt acgcaataag agctatctcc acgatgtaac tttacgggca gagccgtata
     3541 tgtactggat agcagggcaa gttaaaaaac gtaacatgcc tatggaactg gtactactac
     3601 ccatagtgga gagcgctttt gatcctcacg caacgtctgg cgccaatgcc gcaggcatct
     3661 ggcagatcat tccgagcacg gggcgcaatt atggtttgaa acagacccgc aattatgacg
     3721 cgcgtcgcga tgttgttgct tcaacaactg ccgcgctgaa tatgatgcag cgtctgaaca
     3781 aaatgtttga cggcgactgg cttctgaccg tagcggctta taacagcggc gaaggtcggg
     3841 tcatgaaggc aattaaaacg aacaaagcgc gtgggaaatc cacggacttc tggtcgttac
     3901 cgttgccgca ggaaacgaag cagtacgtgc ctaaaatgct ggcattgagt gatattctca
     3961 aaaacagcaa gcgttatggc gtacgtctgc caacgaccga tgaaagccgt gctctggcgc
     4021 gtgtgcacct gagtagcccg gttgaaatgg cgaaggttgc agatatggcg gggatttccg
     4081 tcagcaagct gaagacattc aacgctggcg tgaaaggctc cacgctgggc gcaagtgggc
     4141 cgcagtacgt gatggtgcca aagaagcatg cagatcaact gcgtgaatct ctcgcttcag
     4201 gcgaaattgc tgctgtacag tcgacgctgg ttgccgacaa tacgccgctt aacagccgtg
     4261 tttacaccgt acgctctggc gacacgcttt caagcatcgc ttcacgtctc ggcgtaagca
     4321 ccaaagattt gcagcagtgg aacaaactgc gcggatctaa gctgaagcca ggccaaagtt
     4381 tgacgattgg tgcaggcagt agcgcacagc ggttggcaaa caacagcgat agcattacgt
     4441 atcgtgtgcg caaaggcgat tcgctttcaa gcattgctaa acgccacggc gtgaacatca
     4501 aagatgtgat gcgctggaac agcgacactg cgaatctgca accaggcgat aagctgacgt
     4561 tgtttgtgaa aaacaacaac atgccagatt cctgacaaac cagataataa aaaaggcacc
     4621 gattcccccg gtgccttttt tatttatgcc gctttatgtg cttctaccat gatgatatca
     4681 ctggtgaaag agccatcagt ctgcaaggaa aaatacgttc tcacctctgt cgatgcgctc
     4741 tgttggtaaa tgcgaatagc gtctactaac gcttctggcg tacgcattct cacgacccat
     4801 gaagaaaatt ccagcggtaa cttatctgta attaaattat caactatcag attggcttca
     4861 ttgattaacg tcaaccacgc accgctggcg tagtttcgta cgtgagaggt atcgcgtaat
     4921 gcttctaccg cctgtaacca gatgtcgcgc actgggtgac ccggagacat tacatccatc
     4981 acaatcagcc taccgccagg tttcaatatc ctattcactt ctcgcagtgc tgcaccaaca
     5041 tcatgccagt gatgggcaga ataacggctg ataacaatat caaatgcgtt atcggcaaat
     5101 ggcagacttg cggcatatcc ctggcgggtg gtgatatttt tcagttgccg ggcttcggcg
     5161 gcttgtgcca cgacatccag catgtgggca gataagtcat acgccaccac cgcgctcacg
     5221 ttttgcgcag cgccaaacgt tggtctgccc tgcggcagac ctgacatacc ttatttatca
     5281 ggcttgccgg gactgaattc aacgagtagc ggattgtgat cggaagcgcg cgtaaccagt
     5341 acagaagctt cactgacgtt cagaccacgg tagaaaacaa aatcgagcgg gcgaccaaac
     5401 gcccgacggc gctgatcatc ggtaaaacgc acctggcgca gcgacatttc ccgcgcaaag
     5461 cgatataacg cgttcatcct tctacggctc caggcattga aatctcccgc cataatgacc
     5521 gggccgctgt ggtgagctat ctgatcacca ataggaagta actgcttact atagacatcc
     5581 acgcccagac tgaagttgac ggcgtgtata ttaaccacca tcaacaggcg ggtgtcaggt
     5641 aatggataga ccgtcaccag tgccgacttc gccagacgca aaatgggttc tcgttcacgt
     5701 aacgggcagc aatacactgg atgtgccgcc gaaagggtca ttacgccaga aggatgttgt
     5761 ggcagcacga aagcgggtac ctgatcggcg gcaagatagt tagcggtcgc aaactgtacc
     5821 aactctggcg ttgtttgcgc ttcctgcaat aacaccagat gtgcgtcttt gccgtaattc
     5881 tttaataccg acaaccattc agcgcgttgc tgtttgtata tgttccacac caggatccga
     5941 atacgctctt cggtacttaa cggttctccg ggtggtaatg cctggccgat gctcgcaaaa
     6001 gaccccggcg gtaagatcct ctccgcaggt tgtccggcaa catagcgcat ggcataggtg
     6061 ttttttcgca cttttgactt tgaaacctct attacgtcaa cccttccgca ctcgggaggg
     6121 ttctactgtt atagggattt tagctcactc tttcaacgag caattgtata ttgttatgta
     6181 agcaagtgct tacaccggtc agaaatttgc tcattattga gaacattaaa tcggatgaca
     6241 tatcacgcat cctgatggtt taatgacaaa taaattcaaa atatctcatc tattttgcaa
     6301 caggagcaaa aatgaaagcc acgtcggaag aactcgccat ttttgtttcg gtcgtcgaaa
     6361 gcggcagctt tagccgggca gcggaacaat tagggcaagc aaactcagcg gtaagccggg
     6421 cggtgaaaaa gctggaaatg aaacttggcg ttagcctgct taatcggacc acgcggcaac
     6481 ttagcctgac ggaagaaggc gagcgttatt ttcgtcgcgt acagtcaatt ttgcaggaga
     6541 tggcagcggc agaatcagaa attatggaga cgcgtaatac accgcgtgga ctgttacgga
     6601 tcgatgccgc aactccagtg gtgctgcact ttctgatgcc gttaattaag cctttccgtg
     6661 aacgctatcc ggaagtcact ttatcgctag tctcctccga aacgattatt aatttgatcg
     6721 aaagaaaagt ggatgtcgcg atacgcgctg gtacgttaac ggactccagc ttacgtgcca
     6781 ggccgttatt taacagttat cgaaaaatta tcgcctcccc cgattatatt tcccgctacg
     6841 ggaagccaga aacgatcgac gatttaaagc aacatgtttg cctgggattc actgaacccg
     6901 cttccctcaa tacctggccg atagcctgta gtgatggaca attacatgag gtgaagtacg
     6961 gtttgtcatc caatagtggg gaaacactga aacagctttg cctgagtggc aacgggattg
     7021 cgtgtttgtc cgactacatg atcgacaaag aaatcgctcg cggagaattg gtggagttaa
     7081 tggcagataa agtgttgcca gtggaaatgc cattcagtgc cgtctattac agcgaccgtg
     7141 cggtaagtac gcgcatccgg gcttttatcg atttccttag cgagcatgta aaaacagctc
     7201 ccggaggagc tgtcagagag gcttaatccc attcaggagc cagaccttcc gggctaacca
     7261 ggcggtcgtt gcaatccagt gcggcgatcg cttttttatc ttcggcatca agctgtaaat
     7321 tttgtgcctt aagattactt tccaggtttt tacgtttagt agaagaagga attactgagt
     7381 aaccttcccc catagcccac gccagaatca cttgtgccgg agtcgcattg tgtttagctg
     7441 caatacgagc aataacctca tctttcagtg ccttaccata agccagcgtc atataggaag
     7501 tgatatgaat accgtgctgt ttagcccagg caaccacttt acggttttgc agataaggag
     7561 agagttcaat ctggttggta gcgatgtttt cagcgccaac agcagcaatc gctttttcca
     7621 tcaacgggat cgtgaagttg gaaataccga tctcacgtgt cagaccttgt tttttggcct
     7681 ccagcagcgc ctgcataaac tcttcaacag agacttcatc gtttggtgac ggccagtgga
     7741 ttagcgtcag atcaacataa tcggtacgca atttttgcag gctctctttt agactcggga
     7801 tcaatttgtc tttgctgaga ttttcaatcc agattttagt ggtgatgtag agttcatgac
     7861 gtggcacgcc actttctgca atcgcatgac ctactgcggc ttcgttatca tagatttgtg
     7921 cagtatcaat tgcgcgataa ccaagttcaa gcgccgtttt cacagatgaa ataacaacgt
     7981 cgtctttgag acggaaagta cctaaaccaa atgcagggat agccataata tgcctctttt
     8041 aatgctattt tcgttaactg aggtgattat gggcttcaga ctgttgcgga aaaagagcat
     8101 aaaatgcaga ggatttttgc gattctggca ataatagata tacagacgaa aaaaaacctc
     8161 gagcgttaac tcgaggcttc ttaataagtg gcggaacgga cgggactcga acccgcgacc
     8221 ccctgcgtga caggcaggta ttctaaccga ctgaactacc gctccaccga attcttttac
     8281 aaccaccggt tttatgaccg gcttactgct taatttgatg cctggcagtt tatgaatcac
     8341 ttcgggctgt tgcttcgcaa cgttcaaaag ctttcgcttt tgtcctactc tcttatcgag
     8401 tagtgaactg cctcaaaggt cagtgccatt cttc
//
LOCUS       DADMKF010000161         8326 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.164, whole genome shotgun sequence.
ACCESSION   DADMKF010000161 DADMKF010000000
VERSION     DADMKF010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8326
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.164"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            267..1925
                     /locus_tag="JNC24_003440"
     CDS             267..1925
                     /locus_tag="JNC24_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HAY9160110.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNMEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIVTTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(1922..2878)
                     /locus_tag="JNC24_003441"
     CDS             complement(1922..2878)
                     /locus_tag="JNC24_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF535 domain-containing protein"
                     /protein_id="HAY9160111.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRRSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            3029..4144
                     /gene="macA"
                     /locus_tag="JNC24_003442"
     CDS             3029..4144
                     /gene="macA"
                     /locus_tag="JNC24_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HAY9160112.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSQQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            4141..6087
                     /gene="macB"
                     /locus_tag="JNC24_003443"
     CDS             4141..6087
                     /gene="macB"
                     /locus_tag="JNC24_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HAY9160113.1"
                     /translation="MTPLLELKDIRRSYPAGNEQVEVLKGISLDIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDVATLDADALAQLRREHFGFIFQRYHLLSHLT
                     AEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTAT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(6160..6384)
                     /gene="cspD"
                     /locus_tag="JNC24_003444"
     CDS             complement(6160..6384)
                     /gene="cspD"
                     /locus_tag="JNC24_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HAY9160114.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            6789..8027
                     /locus_tag="JNC24_003445"
     CDS             6789..8027
                     /locus_tag="JNC24_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000092881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAY9160115.1"
                     /translation="MSSAIHRLSDTLLRKLSGSPTTKNAFFNDGGNLSVRHSTSGLLT
                     WYFTYRAGTGRQVSPERLRLGNYPDLSLKAAREKAAQCRAWLAEGKNPRYELNRAVQD
                     ALAPVTVKEALTYWLESYAKEKRTDYESLKSRINKHIISQIGALPLEKCELRHWLACF
                     DQMAKRSPVSAGFLLQVCKQALKYCRKRRYAISNVLDDMVVGDVGKKAEISERVLTNK
                     ELGELLRALDEKIYPPYYSALIRLLIVFGCRATELRRSEVQEWDFKEMLWTVPKEHSK
                     TKVAIFRPIPEAILPFVTKLVEQNRHTGLLLGELKGQSSVSEYGRTAHRRINQAPWTL
                     HDIRHTFTTMLNDLGVDPHVVEQLTAHQLPGVQRVYNHSRYLDAKRDALNLWVERLEL
                     LQNNDEKIVVMTPRIYSQNS"
BASE COUNT         2057 a         1960 c         2233 g         2076 t
ORIGIN      
        1 ggttgcccca tatagtcatt tgtctgattg acagtgtagt gcacgcaaaa gatttaatcc
       61 tttaggcgta ataaaaaata atatatcatg ctaattattt gattttgttg tttttgcaga
      121 cttatcagca agagggagta taacgcgatt attcgctcat ttttcagaca tttgccatgc
      181 ttaaatgtga tgtcatcacg tattagcaag gcctttcccg ttatactgcc agcgtaaagg
      241 ataagtcaca tatttctgga ggggatatga ttcttgagcg cgttgaaatt gtgggttttc
      301 gcggtatcaa ccgtttgtcg ttgatgctgg aacaaaacaa cgtcctgatt ggggagaacg
      361 cgtggggtaa atccagcttg ctggacgcct taaccctgct gctatcgcca gaatcagatc
      421 tctaccattt tgagcgcgac gatttctggt tcccgccggg agatatcaac gggcgagaac
      481 atcatctgca tattattttg accttccgcg aatcgctgcc aggccgacat cgggttcgcc
      541 gttatcggcc gctggaagcg tgctggacgc catgcaccga tggctatcac cgtatttttt
      601 atcgtctgga aggggagagt gcggaagacg gcagcgtgat gacactgcgc agttttctcg
      661 ataaagacgg acatccgatt gatgtcgagg atattaacga tcaggcacgc catctggtgc
      721 gtttaatgcc ggtgctgcgc ttgcgtgatg cccgttttat gcgccgtatt cgtaacggca
      781 cggtgccaaa tgtccctaat atggaagtca ccgcgcgcca gctcgatttc ctcgcccgtg
      841 agttatcctc acatccgcaa aatctctctg atgggcagat tcgtcaggga ctttccgcaa
      901 tggtacagct gcttgagcat tatttctctg agcagggggc cggacaggcg cgatatcgtt
      961 taatgcggcg gcgagccagc aatgagcaac gaagctggcg ctatctggat atcatcaacc
     1021 ggatgattga ccgacctggt gggcgctcgt atcgggttat tttgctcggc ctgtttgcta
     1081 ctttgttgca ggcaaaaggc acattgcgac tggataaaga cgcccgtcca ttgttgctga
     1141 tcgaagatcc agaaacccgt ttacacccca ttatgctttc agttgcctgg catctgttga
     1201 atcttctgcc attgcagcgc attgtcacca ccaactcggg tgagttgctt tcgttaacgc
     1261 cggtagagca tgtttgccga ctggtacgtg agtcctcgcg cgttgccgcc tggcgtctgg
     1321 ggccgagtgg cttgagtacc gaagatagcc gacgcatatc ctttcacatt cgttttaacc
     1381 gtccgtcatc gctgtttgca cgctgctggt tgctggtgga aggggaaacg gaaacctggg
     1441 ttatcaatga actggcgcgt cagtgcggac atcattttga tgccgaaggg atcaaggtca
     1501 ttgagtttgc ccagtccggg ctaaagccac tggttaaatt tgcccgccga atggggattg
     1561 aatggcatgt actggtcgat ggcgatgaag cagggaagaa atatgccgct acggtacgca
     1621 gcctgttgaa taacgatcgg gaagccgaac gagaacattt aacggcgtta ccggcgctgg
     1681 atatggaaca ttttatgtat cgccagggat tttccgatgt gttccaccgc gtggcgcaaa
     1741 tcccggaaaa tgtaccgatg aatctacgca aaattatctc gaaagcgatc catcgctctt
     1801 ccaaacccga tcttgccatt gaagtggcaa tggaggcagg acgtcgtggt gtggactccg
     1861 taccgacgct gctgaaaaaa atgttctcac gcgtgctgtg gctggcgcgc ggtcgcgcgg
     1921 attaaccgcg aaacatcgtg gccatttgtg gctgaatagc gtcgagcatc tcatagcgcc
     1981 gacggtattc agcccgtttt ttactggcga tttcggcaat ctcttttcgt gctatctgtg
     2041 ctggaaggcg gtaatggcgt tcagcatcac atacgccgcc aaccgattcc cagaaagcgt
     2101 tgtaatcagc gtggatcttg ccttctttat cgcgataacg caggctgcgg taaatatgcg
     2161 tttcattgct gacggcaata atctgctcta cctgcaaacg ttgggcaaac agacaggccg
     2221 cttccatcac gaggcgtttg ggaaatagcc cgtggcaggc tttcgtcgca ttctggattt
     2281 cctgatgtgg aatttcccat tttgcgcctt gcagtccgcc aataaacatc gttcttttcc
     2341 cctgatattc acacagggta aacgtgatct ctgccagagg aataccttcg ctgttgcgaa
     2401 acaggattgt gctgtcacct tctttatcca ttgagatcat catggtcagc tcaagcgtga
     2461 actgctcgcc gtttttgcct tccagcttcg ccagttgcag cccgggggta ttcaaatata
     2521 agctgaattc ttccgccgac atacatccgc ggagtaacgc ataatggtaa cgtaacgcct
     2581 ccagcaattg cttacggcta agattcgccg caaggtaagg gcgatgcaga cgcacaggca
     2641 gtcgcggttg gcgcgttaac aacacattga gattaggcca gtgggaaagt tcgttcatcc
     2701 actcaacgct taaacgcggc ataatcaacg agcgcagcaa aaatttctgg cgaaaactac
     2761 ggcgatgcca gaatttaccc ggccgacact gtccacgtgc cagactaaga aaaagtgaca
     2821 ggctgctgag agattcagat ggcgtaaagg tccgttcagt tagctgcgac atattcatga
     2881 aatcaatggt tatacatgac gtcgatttca ccattgcgta tcttaaccaa acatcaatag
     2941 tgtgattact aacgtaaatt ttatggtttt gttgatattt cgttgaagtt aatgacccgg
     3001 attggcatat ggagtattca gaatatttat gaaaaagcgg aaaaccgtga agaagcgtta
     3061 cgttattgcg ctggtgatag tcatcgccgg actgattacg ttatggagaa ttcttaacgc
     3121 acccgtgccg acttatcaga cactgattgt gcgccccggt gatttacagc aaagcgtgct
     3181 ggcgaccgga aagctggacg cgctgcgtaa ggttgacgtg ggcgcgcagg tcagcggtca
     3241 gttgaaaacg ctgtcggtgg cgattggcga taaagtaaaa aaagaccagc ttttaggggt
     3301 cattgatcct gaacaggctg aaaaccagat caaggaggtc gaagcaacgc tgatggagct
     3361 acgcgcgcag cggcagcagg cggaagcgga gctgaaactg gcgcgggtga cgtattcccg
     3421 tcagcaacgt ctggcacaaa cgcaggcggt ttcacagcag gatctcgaca ccgccgcgac
     3481 ggagatggct gtgaaacagg cgcaaattgg caccattgac gcgcaaatca agcgcaatca
     3541 ggcttctctc gatacggcta aaaccaatct cgattacact cgcatcgttg ccccgatggc
     3601 cggggaagtc acgcaaatca ccactctgca aggccagacg gtgattgccg cacaacaagc
     3661 accgaacatt ctgacgctgg cagatatgag caccatgctg gtaaaagcgc aggtttctga
     3721 agcggatgta atccacctga agccggggca aaaagcctgg tttacggtac ttggcgatcc
     3781 actgacgcgc tacgaggggc aaatcaagga tgtactaccg acgccggaaa aggttaacga
     3841 cgctattttc tattacgccc gttttgaagt ccccaacccc aatggtttgc tgcggctgga
     3901 tatgactgcg caagtgcata ttcagctcac cgatgtgaaa aatgtgctga cgatccctct
     3961 gtcggcgtta ggcgatccgg ttggcgataa tcgttataaa gtcaaattgt tgcgtaatgg
     4021 cgaaacacgc gagcgtgaag tgacgattgg cgcacgtaac gataccgatg ttgagattgt
     4081 caaagggctg gaagcgggcg atgaagtggt gattggtgag gccaaaccag gagctgcaca
     4141 atgacgcctt tgctcgaatt aaaggatatt cgtcgcagct atcctgccgg taatgagcag
     4201 gttgaggtgc tgaagggcat cagcctcgat atttatgcgg gtgagatggt cgcgattgtt
     4261 ggcgcttcgg gttccggtaa atcgaccctg atgaatattc tcggctgcct ggataaggcc
     4321 accagcggca cctatcgcgt agccggtcag gatgttgcca cgctggacgc cgatgcgctg
     4381 gcgcaactgc gccgcgagca tttcggcttt attttccagc gttaccattt gctttcgcat
     4441 ttaaccgccg agcagaacgt tgaagtaccc gccgtctatg ctggtcttga gcggaaacag
     4501 cgactgcttc gtgcccagga gttgctgcaa cggctggggc tggaagaccg tacagagtat
     4561 tatccggcac agctttcggg tggtcagcaa cagcgcgtca gcatcgcgcg ggcattgatg
     4621 aacggcggtc aggtaattct tgccgatgaa ccaaccggcg cactggacag ccattctggc
     4681 gaagaggtga tggcgatcct gcatcagctg cgcgatcgcg ggcatacggt gattatcgtc
     4741 acccacgatc cgcaggtcgc tgctcaggcc gagcgggtga tcgaaattcg cgacggcgaa
     4801 attgtgcgca atcctcccgc cattgaaaaa gtgaatgttg ctggcgggac ggagcctgtt
     4861 gtcaatacag tgtctggctg gcggcagttt gtcagcggtt ttaacgaggc gctgacgatg
     4921 gcatggcggg cgctggcagc gaataaaatg cgtactttac tgaccatgct ggggattatt
     4981 atcggtattg cgtcggtggt ttccattgtc gtggtgggtg acgccgccaa acaaatggtg
     5041 ctggcggata ttcgttctat tggtacgaat actattgatg tctatcccgg gaaagatttt
     5101 ggcgatgacg atccgcaata tcagcaggcg ctgaagtacg acgacttaat cgccatccaa
     5161 aaacaaccgt gggtcgcctc agccacacct gccgtctcgc aaaacctgcg cctgcgttat
     5221 aacaatgttg atgttgctgc cagtgccaat ggcgtgagcg gcgattattt taatgtctat
     5281 ggcatgacct tcagtgaagg aaacaccttt aatcaggagc agctgaacgg tcgtgcgcag
     5341 gtcgtggttc tcgacagtaa tactcgccgc cagcttttcc cccataaagc agatgtggtt
     5401 ggcgaggtga ttctggtcgg caatatgccc gccagagtca ttggtgtggc ggaagaaaaa
     5461 cagtcgatgt ttggtagcag taaagtgctg cgtgtctggc taccttacag cacgatgtcc
     5521 gggcgagtta tgggccagtc gtggcttaac tccattactg tcagggtgaa agaaggattt
     5581 gacagcgccg aggcggaaca gcaactcacg cgtttacttt cactgcgcca cggtaagaag
     5641 gatttcttta cctggaacat ggacggcgtc ttgaaaactg ttgaaaagac cacacgtact
     5701 ttacaactgt ttctgacgct ggtggcggtg atttcgctgg tggtgggtgg tattggtgta
     5761 atgaatatta tgctggtgtc agtgaccgag cggacgcggg aaattggcat tcgcatggct
     5821 gtaggtgcgc gagcaagcga tgttttgcaa cagttcctga tcgaagccgt actggtttgc
     5881 ctggtcggtg gcgcgttggg aataactctg tcactgttaa ttgctttcac cttgcagctt
     5941 ttcttacccg gctgggagat tggtttttca ccgttggcac tgctgctggc gtttctctgc
     6001 tcgacggcca ccgggatttt atttggatgg ttacccgcac gaaatgcggc acgactggat
     6061 ccagtagatg ctctggcacg agagtaattt ttgagataaa aatgccagcc gatcgggctg
     6121 gcattttgcc tttaggatgt acacaatgag acagaagagc tatgcgactg ccgcttctac
     6181 ttcgacgggc acaataacac tggcgtgatt gccttttggc ccctggtgga catcaaactg
     6241 aacggattgt ccagctttta gcgttctata accatccatc tgaatggtgg aataatgagc
     6301 gaaaatatct tcgccgccgc cttcagggca gatgaaacca aaccctttgg cattgttgaa
     6361 ccacttaaca gtaccctttt ccatgcttcg acatccttcg caaatcttat acaagtaaga
     6421 tggaataaac cggggtcaga gagggggctg ttcaaaacct cgccaactct agaaatacaa
     6481 tttagagaat tagggcgagc cgtcaagcat ttgacagggg ccagggggca ggtatgaatc
     6541 aaaaatttga agcagttaac gctattgaca ggaatgtgac agatgtcgct gatgccaacg
     6601 atagatgata gttatttatc atctagtgtt cgcgggtgtt cgtggtagtg ttttgtgtgc
     6661 attcatcgct atgattataa aggaaaattt tgttttcctt gttcgctctt gttcgtcacg
     6721 gtttgccctt gtacgatgca cggtgtagtc aatggtgtag ttaattttga tgaccggaaa
     6781 cgcgaaacat gagcagcgca attcatagac ttagcgacac tcttttacga aaattaagcg
     6841 gatcaccaac cacaaaaaac gcctttttta atgacggtgg caatcttagc gtaagacatt
     6901 ccaccagcgg cctgttaacc tggtatttca cttacagggc cggaactggc aggcaggtat
     6961 caccggaacg cctgagactg ggcaactatc ccgatctgag cctgaaagca gccagggaaa
     7021 aagcggcaca gtgtcgcgcc tggctggctg aggggaaaaa tccgcgctat gagcttaatc
     7081 gtgctgtaca ggatgcgtta gcccccgtga cagttaagga agcgctcact tactggcttg
     7141 aatcgtatgc aaaggaaaag cgcactgact acgaatcact gaaaagccgg atcaataaac
     7201 acataatcag ccagattggc gctttgccgt tggaaaaatg cgagctacgg cactggttgg
     7261 catgttttga ccagatggca aagcgaagcc cagtatctgc cggatttttg ctacaggtat
     7321 gcaaacaggc gcttaagtac tgccgaaaac gacgatacgc aatcagcaac gttcttgatg
     7381 atatggttgt cggtgatgtg ggaaaaaaag cagaaataag cgagcgcgta ctaacaaaca
     7441 aggagcttgg ggaattactc cgcgccctgg atgaaaaaat ttacccaccg tactacagcg
     7501 ccctgattcg cctcctgatt gtcttcggat gccgtgccac tgagctaaga cgctctgagg
     7561 tccaggaatg ggattttaaa gaaatgctct ggaccgtgcc gaaagaacac agcaaaacga
     7621 aggtagcaat attcaggcca ataccggaag cgatcttgcc gttcgtaacg aagctggtgg
     7681 agcagaacag gcacacggga ttattgctcg gagaactgaa agggcaatca tccgtatcag
     7741 agtacggaag aacggcacac agacgcatta atcaagcccc ctggacgttg cacgacatcc
     7801 ggcacacgtt tacaacgatg ctgaacgatt taggcgtgga tcctcatgta gtggagcagc
     7861 taaccgcgca ccagttgcca ggagtgcaac gagtctataa tcactcccgt tatctcgatg
     7921 caaaacgtga cgctcttaat ctatgggttg agcgtctcga gcttctccag aacaatgatg
     7981 aaaaaatcgt tgttatgacc ccgcgaattt actcccaaaa ttcttgacaa attacggctg
     8041 tttttcttgc gaaaaatggc cctctaaaat aaatcgtcga atatcggcaa atatcgacga
     8101 tttatatgtg tcaactatcg aaaaaaattg aaatgtcttc ttctgagttc ttattctctg
     8161 tttcttgatt attccaatac ctgatggttt atctatttgt ttttaatgag gtaattcagg
     8221 tgtcatgcca aatggcgaca ttttttgttg cgtcgcttaa aaactcgtga cacgatttta
     8281 aacactaacg agcttctacg aaccttagcg aacaaagcct ttagca
//
LOCUS       DADMKF010000162         8290 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.165, whole genome shotgun sequence.
ACCESSION   DADMKF010000162 DADMKF010000000
VERSION     DADMKF010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8290
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.165"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            122..445
                     /gene="soxS"
                     /locus_tag="JNC24_003446"
     CDS             122..445
                     /gene="soxS"
                     /locus_tag="JNC24_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HAY9160116.1"
                     /translation="MSHQKIIQDLIAWIDEHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            complement(448..2034)
                     /gene="pdeC"
                     /locus_tag="JNC24_003447"
     CDS             complement(448..2034)
                     /gene="pdeC"
                     /locus_tag="JNC24_003447"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HAY9160117.1"
                     /translation="MSHRARHQLLALPGIIFLVLFPIILSLWIAFLWAKSEVNNQLRT
                     FAQLALDKSELVIRQADLVSDAAERYQGQVCTPAHQKRMLNIIRGYLYINELIYARDN
                     HFLCSSLIAPVNGYTIAPADYKREPNVSIYYYRDTPFFSGYKMTYMQRGNYVAVINPL
                     FWSEVMSDDPTLQWGVYDTVTKTFFSLSKEASAATFSPLIHLKDLTVQRNGYLYATVY
                     STKRPIAAIVATSYQRLITHFYNHLIFALPAGILGSLVLLLLWLRIRQNYLSPKRKLQ
                     RALEKHQLCLYYQPIIDIKTEKCIGAEALLRWLGEQGQIMNPAEFIPLAEKEGMIEQI
                     TDYVIDNVFRDLGAYLATHADRYVSINLSASDFHTSRLIARINQKTEQYAVRPQQIKF
                     EVTEHAFLDVEKMTPIILAFRQAGYEVAIDDFGIGYSNLHNLKSLNVDILKIDKSFVE
                     TLTTHKTSHLIAEHIIELAHSLGLKTIAEGVETEEQVNWLRKRGVRYCQGWFFAKAMP
                     PQVFMQWMEQLPARELTRGQ"
     gene            2464..2744
                     /locus_tag="JNC24_003448"
                     /pseudo
     CDS             2464..2744
                     /locus_tag="JNC24_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2843..3379)
                     /gene="ssb1"
                     /locus_tag="JNC24_003449"
     CDS             complement(2843..3379)
                     /gene="ssb1"
                     /locus_tag="JNC24_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HAY9160118.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            3633..6455
                     /gene="uvrA"
                     /locus_tag="JNC24_003450"
     CDS             3633..6455
                     /gene="uvrA"
                     /locus_tag="JNC24_003450"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HAY9160119.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(6490..6846)
                     /locus_tag="JNC24_003451"
     CDS             complement(6490..6846)
                     /locus_tag="JNC24_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HAY9160120.1"
                     /translation="MTISELLQYCMAKPGAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(6850..7266)
                     /locus_tag="JNC24_003452"
     CDS             complement(6850..7266)
                     /locus_tag="JNC24_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HAY9160121.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMERFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PLHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(7377..8090)
                     /locus_tag="JNC24_003453"
     CDS             complement(7377..8090)
                     /locus_tag="JNC24_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class B acid phosphatase"
                     /protein_id="HAY9160122.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
BASE COUNT         2063 a         2098 c         2059 g         2070 t
ORIGIN      
        1 tagcagcgct ttaatgcggg gtaatttctt ttccataaat cgctttacct caagttaact
       61 tgaggaatta tactccccaa cagatgaatt aacgaactga acactgaaaa gaggcagatt
      121 tatgtcccat cagaaaatta ttcaggatct tatcgcatgg attgacgagc atattgacca
      181 gccgcttaac attgatgtag tcgcaaaaaa atcaggctat tcaaagtggt acttgcaacg
      241 aatgttccgc acggtgacgc atcagacgct tggcgattac attcgccaac gtcgcctgtt
      301 actggccgcc gttgagttgc gcaccaccga gcgtccgatt tttgatatcg caatggacct
      361 gggttatgtc tcgcagcaga ccttctcccg cgttttccgt cggcagtttg atcgcactcc
      421 cagcgattat cgccaccgcc tgtagtttta ttgcccgcgc gttaactccc gcgcgggtaa
      481 ttgctccatc cattgcataa acacctgcgg cggcatcgcc ttcgcaaaaa accatccctg
      541 gcaatagcgc acgccgcgtt tgcgcagcca gttaacctgc tcctcagttt cgacgccttc
      601 agcgatcgtt tttaacccca ggctgtgcgc cagctcgatg atgtgttccg caatcaaatg
      661 actggttttg tgggtggtca gcgtttcaac aaacgattta tcgattttca aaatatcgac
      721 attcaatgat ttaaggttat gcaagttaga gtagccaata ccaaaatcat caattgccac
      781 ttcgtaacca gcctggcgga aagccagaat aatcggcgtc attttttcga catcaagaaa
      841 tgcatgctca gtcacttcaa atttaatttg ctgcggacgc accgcgtatt gctctgtttt
      901 ctgattgatt cgcgctatca accgtgacgt atgaaaatcg gaggccgaca ggttaataga
      961 aacatagcga tcggcatgtg ttgccaagta cgcgcccaga tcgcggaaga cattatcaat
     1021 aacataatca gttatctgtt ctatcatccc ctccttttct gccagcggaa taaactctgc
     1081 cggattcatt atttgcccct gctcaccaag ccaacgtaac aacgcttcag cgccgataca
     1141 tttttctgtt ttgatatcga ttattggctg gtaataaaga caaagttgat gtttttcgag
     1201 ggcgcgttgc aatttacgtt tgggagataa atagttttgt cgaatacgta gccagagtaa
     1261 tagcagaaca agactcccca aaataccggc gggcaacgca aaaataagat gattataaaa
     1321 atgggttata agacgttgat atgaagtcgc aacaatggct gcaattgggc gttttgtcga
     1381 ataaactgtc gcatataaat agccatttct ttgtacggtt aaatccttca aatgaatcag
     1441 cggagaaaac gttgctgccg aggcctcttt gcttaacgag aaaaaggttt tcgtcaccgt
     1501 atcatacaca ccccattgca atgtcgggtc atcagacatc acttcactcc agaagagagg
     1561 gttgataacc gccacataat ttccccgctg catataggtc attttatagc cagagaaaaa
     1621 aggcgtatcg cggtaataat agatagaaac gttaggttca cgcttataat cggccggtgc
     1681 aatcgtatag ccgtttacag gcgctatcag cgatgagcat aaaaaatggt tatcacgggc
     1741 atagatcaat tcattaatat aaagatagcc acgaataata ttcaacattc gcttttgatg
     1801 ggctggagtg caaacttgcc cctgatagcg ttcagctgca tcgctcacta aatctgcctg
     1861 gcgaatgacc agctcggatt tatccagtgc cagttgagca aaggttcgga gctgattatt
     1921 cacttctgat tttgcccaaa ggaaggcaat ccatagcgaa agaatgatgg gaaagagaac
     1981 taaaaagata atgcccggca acgccagtaa ttggtgtcgt gcacgatgac tcattgctat
     2041 caatttcctg cctgtttata cccggccatt gcaaggtgaa aaccctgggt accgacgtct
     2101 ttagataaat cttacggtat aaatagcact tttgcctgat ttgaaattga tacctttcta
     2161 atcgtcacct ggatgaaaat aattcattga tattcaaaat taatttccta caggaatgca
     2221 gaggcggcgg gaagatatgc ccttaatgcc actgaaaaac cacataatct gtacagcaat
     2281 aaaagtcacg gcctaatatg gctaacaaac aatcaattgt gatatagctc acaaaattaa
     2341 tgaaacaaac agagtgtttc atttttgtgt tctgcaagcc ccgcgatagc cagagtgtac
     2401 cacgcctccc gtgaacaacg ccgcgctgtc cagggcatcg gctcttttca gaggaattgt
     2461 ttgatggcta ccctcaccac tggcgtggtt cttcttcgct ggcagcttct tagtgccgta
     2521 atgatgtttc tggccagcac atcaacatcc gttttcgtcg gtctgattat gtcgggcttg
     2581 cggtgatcag cagcggtctg ggcgttgttt ccgcctgttg gttcgcaatg gggttgcttg
     2641 gcatcacaat ggcggacatc accgctatct ggcacaacat cgagtcggtg atgatagaag
     2701 agatgaatca gacaccgcca caatggccaa tgattttgac ctgatacgta gaagcctcca
     2761 taacggaggc ttctttttta tggctggcag atgctttaat catccacctt aaaacaatat
     2821 aacctattgt tttaatgaca aatcagaacg gaatgtcatc atcaaagtcc atcggcggct
     2881 cgttagacgg cgctgccgga gcggactgct gcgggcgaga ctgcgcgccg ccgctgaact
     2941 gattgccacc ctgcggctgc tgaggctgcc cccaaccgcc ctgcggctga ccaccaccga
     3001 tattaccacc tgccggagcg ccaccaccct gacgaccacc cagcatctgc atggtgccgc
     3061 caacgttcac cacgacttct gtggtgtagc gatcctgacc ggattgatcg gtccatttac
     3121 gggtacgcag ctgaccttcg atataaacct gagaaccttt acgcagatat tcgctggcca
     3181 cttctgccag tttgccgaac agcacaacgc ggtgccattc agtctgttct ttcatctcgc
     3241 cggtcgcttt atcacgccag gattcggaag tagccagcgt aatgttggca actgcgccac
     3301 catttggcat gtagcgtact tccgggtcct gacccagatt accaacgaga ataaccttgt
     3361 ttacgcctct gctggccatg ttcgtgtctc ctgaaaaaaa tcgttctgaa taagtgtaaa
     3421 cgcgcgattg taccattacc aatagcgctt ttactatgtt gtgacctcgg ttccgggaaa
     3481 caaacctggc cagacattgt tacacaacac tccggataat gcattccaat actgtatatt
     3541 cattcaggtc aatttgtgtc ataattaacc gtttgtgatc gccggtagca ccatgccacc
     3601 gggcaaaaaa gcgtttaatc cgggaaaggt gaatggataa gatcgaagtt cggggcgccc
     3661 gcacccataa tctcaaaaac atcaacctcg ttatcccccg cgacaagctc attgtcgtga
     3721 ccgggctttc gggttctggc aaatcctcgc tcgctttcga caccttatat gccgaagggc
     3781 agcgccgtta cgttgaatcc ctttccgcct acgcgcggca gtttctgtca ctgatggaaa
     3841 agccggacgt cgatcatatt gaggggcttt ctcctgccat ctcaattgag cagaaatcga
     3901 cgtctcataa cccgcgttct acggtgggga caatcaccga aatccacgac tatttgcgtt
     3961 tgttgttcgc ccgcgtcggc gaaccgcgct gtccggacca cgacgtcccg ctggcggcgc
     4021 aaaccgtcag ccagatggtg gataacgtgc tgtcgcagcc ggaaggcaag cgtctgatgc
     4081 tgctcgcgcc aatcattaaa gagcgcaaag gcgaacacac caaaacgctg gagaacctgg
     4141 caagccaggg ttacatccgt gctcgtattg atggcgaagt ctgcgatctt tccgatccgc
     4201 cgaaactgga actgcaaaag aaacacacta ttgaagtggt ggttgatcgc ttcaaggtgc
     4261 gtgacgatct tacccaacgt cttgcggagt cgtttgaaac cgcgctggag ctttccggtg
     4321 gtacagcggt agtggcggat atggacgacc cgaaagcgga agagctgctg ttctccgcca
     4381 acttcgcctg cccaatttgc ggctacagta tgcgtgaact ggagccgcga ctgttttcgt
     4441 ttaacaaccc ggcaggtgcc tgcccgacct gtgacggcct tggcgtacag caatatttcg
     4501 atcctgaccg cgtgatccaa aaccccgagc tgtcactggc tggcggtgcg atccgtggct
     4561 gggatcgccg caacttctat tatttccaga tgctgaaatc gctggcagat cactataagt
     4621 tcgacgtcga agcgccgtgg ggcagcctga gcgcgaacgt gcataaagtg gtgttgtacg
     4681 gttctggcaa agaaaacatt gaattcaaat acatgaacga tcgtggcgat acctccattc
     4741 gtcgtcatcc gttcgaaggc gtgctgcaca atatggagcg ccgttataaa gagacggaat
     4801 ccagtgcggt acgtgaagaa ttagccaagt ttatcagcaa tcgcccatgc gccagctgcg
     4861 aaggaacgcg tctgcgtcgg gaagcgcgcc acgtgtatgt cgagaatacg ccgctgcctg
     4921 ctatctccga catgagcatc ggtcatgcga tggaattctt caacaatctc aaactcgcag
     4981 gtcagcgggc gaagattgca gaaaaaatcc ttaaagagat cggcgatcgt ctgaaattcc
     5041 tcgttaacgt cggcctgaat tacctgacac tttcccgctc ggcagaaaca ctttccggcg
     5101 gtgaagccca gcgtatccgt ctggcgagcc agattggtgc gggcctggtt ggcgttatgt
     5161 atgtactgga cgagccgtct atcggcctgc accagcgcga taacgagcgc ctgttgggta
     5221 cgcttatcca tctgcgcgat ctcggtaata ccgtgattgt ggtggagcat gacgaagacg
     5281 caattcgcgc cgctgaccat gtgatcgata tcggtcctgg tgcgggtgtt cacggcggtg
     5341 aagtggtcgc agaaggtccg ctggaagcga ttatggcggt gcctgaatcg ttgaccgggc
     5401 agtacatgag cggtaaacgc aagattgaag tgccgaagaa acgcgttccg gcgaatccag
     5461 aaaaagtgct gaagctgaca ggtgcacgcg gcaacaacct gaaggacgtg acgctcacgc
     5521 tgccagtcgg tctgtttacc tgcatcacag gggtttcagg ttccggtaaa tcgacgctga
     5581 ttaacgacac actgttcccg attgcccaac gccagttgaa tggtgcgacc atcgccgaac
     5641 cggcaccgta tcgcgatatt caggggctgg agcatttcga taaagtgatc gatatcgacc
     5701 aaagcccaat tggtcgtact ccacgttcta acccggcgac ctataccggc gtgtttacac
     5761 ctgtgcgcga actgtttgcg ggcgtaccgg aatcccgtgc gcgtggttat acgccaggac
     5821 gtttcagctt taacgtccgt ggcgggcgct gcgaagcctg tcagggcgac ggtgtgatca
     5881 aagtggagat gcacttcctg ccggatatct acgtgccgtg cgatcagtgc aaaggtaaac
     5941 gctataaccg tgaaacgctg gaaattaagt acaaaggcaa aaccatccac gaagtgctgg
     6001 atatgaccat cgaagaggcg cgtgagttct ttgatgcggt gccagctctg gcgcgtaagc
     6061 tgcaaacgtt gatggacgtt ggtctgacgt acattcgcct ggggcagtcc gcaaccacac
     6121 tttctggtgg tgaagcccag cgcgtgaagc tggcgcgtga gctgtcaaaa cgcggcaccg
     6181 ggcagacgct gtatattctc gacgagccga ccaccggtct gcacttcgcc gatattcagc
     6241 aactcctcga cgtgctgcat aaactgcgcg atcagggcaa taccattgtg gtgattgagc
     6301 acaatctcga cgtgatcaaa accgctgact ggattgtcga cctgggacca gaaggcggca
     6361 gtggcggcgg cgaaatcctc gtctccggta cgccagaaac cgtcgcggag tgcgaagctt
     6421 cgcatacggc acgcttcctc aagccgatgc tgtaatcgtt aaggccgctt tctgagcggc
     6481 cttttccttt cagagttgta ccagcaattt acgcttttct tccggtagta aattcactgc
     6541 ctgctgataa gacgcatcca ccagataata gatttgcgaa tccggcagcg aaccgtcgag
     6601 atacacggtg ctccagtgcg ctttattcag atggcggcta ggacgcacat cgctgtgctg
     6661 ctgacgtagc agctccgcca gctccgggct ggttttcagc gaaacagctg ggcgattttc
     6721 tacttctttc accatcgcaa acagtacatc ttccactttg atctgcgtcg ctttccagtc
     6781 gttatgcacg ctctgttctg cgcctggttt tgccatgcaa tattgtagca actccgaaat
     6841 ggtcattttt tactcccctt gtagtgtcgc gatgatgcga cgcgatccgc cgtggatgcg
     6901 atgttccccc agccagatgc cttgccaggt gccggtctga atacgccctt tatgtaacgg
     6961 caatacaagc gatgttccca gcattgagga tttgatatga gaaggcatat cgtctgctcc
     7021 ctcatagtca tgctcataat ttccgttgtc gggaacggtg cggaggaaaa aacgctccat
     7081 gtcgtggcgt acggtgggat cgcagttctc attaagtgtc agagaggcgg aggtatgttg
     7141 cagcaacaga tgcagtaagc cgatgttaac gcgcggcata tcagccagct gattcagaat
     7201 ttcatccgtt accagatgaa acccacgaga tttggcgcta agcgtgagcg tcttttgata
     7261 ccacatgtgc tgctccttga taaaactctc ttaatcagtt tgcagcaaga cagcgaaagg
     7321 ataaaggtgt gattaaaaaa acagcattcg ggagagcatc acgctctccc gctctgtcag
     7381 tattctgaat tgacgatcac ctcttcacca aacgcacccg cttgtggcaa gggtttgtag
     7441 gtagagttgg aggcgcgcag aatgcggata ccacgagcgc cgacatcgcg tgcggcggta
     7501 atatcgttat cggaatcgcc ataaaaaatt cggatatttt tatcctgcag ccattgcgat
     7561 tttgtatttt gccctggttt atcgcccgca aagattaccg gattcatgtt ggtggcagga
     7621 atatgaaaat tatccgccag cgtttttgaa accgtttctg ttttcgtcgg gctacgacca
     7681 gtcacaaaga agatcgcgtc accgcggcgt acatgcatat caatcagctg gcgagcgacc
     7741 tcttttggaa tgctgaattc atcccagcca ttattcattt tttcccagaa cacaggattt
     7801 ttcagataat cttcgctttc tggcgagaag gtttttttgc cgcgccagaa ccccggactg
     7861 gaaaaaagta ccgtgtcatc gatatcaaac cccaccgcca ttggcggacg ccctgcgagg
     7921 ctattttcaa tttgtgcgac cgaaacccaa tgaattggtg cctgttcagc aagcctggca
     7981 acgttagtcc cagggttaag cggtgaagga gatgaggcca gggcaacagc ggaactgttt
     8041 agagcgaaca ataagcaaac ggcactgatt gcctgtgtga tcttgcgcat atttttccct
     8101 aaatagtcag tatgttgaaa ctttttattg tgagatttgt tgcaaaaacc atctgaccat
     8161 aacgccagct gccggggaaa ggaaggatta tctgcggttt ttgtgatgaa aagcaggatg
     8221 attatattta acccagtaaa tacgttcctg tctttttttg tataaaaaat gtttattact
     8281 cgatgtaagg
//
LOCUS       DADMKF010000163         8273 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.166, whole genome shotgun sequence.
ACCESSION   DADMKF010000163 DADMKF010000000
VERSION     DADMKF010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8273
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.166"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            52..936
                     /locus_tag="JNC24_003454"
     CDS             52..936
                     /locus_tag="JNC24_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HAY9160123.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHNEDADGVALTFELTQSEETKKFCPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSISMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(987..1841)
                     /gene="yeaE"
                     /locus_tag="JNC24_003455"
     CDS             complement(987..1841)
                     /gene="yeaE"
                     /locus_tag="JNC24_003455"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal reductase YeaE"
                     /protein_id="HAY9160124.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLRDNVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHRSGSFAFEETVAAMEKLIAQGKIRRWGVSNLDYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHA
                     HNISSAQVLLAWVISHQGVMAIPKAATIAHVQQNAAVLEVELSSAELTMLDKAYPAPK
                     GKTALDMV"
     gene            complement(1931..2677)
                     /locus_tag="JNC24_003456"
     CDS             complement(1931..2677)
                     /locus_tag="JNC24_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HAY9160125.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLTGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            3113..5047
                     /gene="yeaG"
                     /locus_tag="JNC24_003457"
     CDS             3113..5047
                     /gene="yeaG"
                     /locus_tag="JNC24_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HAY9160126.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMKGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQQIEREQFPQEQAERYLEFLKGYLIPKYAEFIGKEIQTAYLESYSEYGQNIFDRYVT
                     YADFWIQDQEYRDPDTGQLFDRESLNAELEKIEKPAGISNPKDFRNEIVNFVLRARAN
                     NSGRNPNWTSYEKLRTVIEKKMFSNTEELLPVISFNAKTSTDEQKKHDDFVDRMMEKG
                     YTRKQVRLLCEWYLRVRKSS"
     gene            5160..6443
                     /locus_tag="JNC24_003458"
     CDS             5160..6443
                     /locus_tag="JNC24_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HAY9160127.1"
                     /translation="MTGFIDRRLNGKNKSMVNRQRFLRRYKAQIKQSISEAINKRSVT
                     DVDSGESVSIPTEDISEPMFHQGRGGLRHRVHPGNDHFVQNDRIERPQGGGGGSGSGQ
                     GQASQDGEGQDEFVFQISKDEYLDLLFEDLALPNLKQNQQRQLTEYKTHRAGYTANGV
                     PANISVVRSLQNSLARRTAMTAGKRRELHALEENLAIISNSEPAQLLEEERLRKEIAE
                     LRAKIERVPFIDTFDLRYKNYEKRPDPSSQAVMFCLMDVSGSMDQSTKDMAKRFYILL
                     YLFLSRTYKNVEVVYIRHHTQAKEVDEHEFFYSQETGGTIVSSALKLMDEVVKERYNP
                     AQWNIYAAQASDGDNWADDSPLCHEILAKKLLPVVRYYSYIEITRRAHQTLWREYEHL
                     QSTFDNFAMQHIRDQDDIYPVFRELFHKQNATAKD"
     gene            6590..8065
                     /locus_tag="JNC24_003459"
     CDS             6590..8065
                     /locus_tag="JNC24_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HAY9160128.1"
                     /translation="MIQSTRISMGLFFKYFLSLTKIDPGQNYISLPSIKSSTHIALLF
                     MVSMGTQKLKAQSFFIFSLLLTLILFCITTLYNENTNVKLIPQMNYLMVVVALFFLNA
                     VIFLFMLMKYFTNKQILPTLILSLAFLSGLIYLVETIVIIHKPINGSTLIQTKSNDVS
                     IFYIFRQLSFICLTSLALFCYGKDNILDNNKKKTGILLLALIPFLVFPLLAHNLSSYN
                     ADYSLYVVDYCPDNHTATWGINYTKILVCLWAFLLFFIIMRTRLASELWPLIALLCLA
                     SLCCNLLLLTLDEYNYTIWYISRGIEVSSKLFVVSFLIYNIFQKLQLSSKLAVHDVLT
                     NIYNRRYFFNSVESLLSRPVVKDFCVMLVDINQFKRINAQWGHRVGDKVLVSIVDIIQ
                     QSIRPDDILARLEGEVFGLLFTELNSAQAKIIAERMRKNVELLTGFSNRYDVPEQMTI
                     SIGTVFSTGDTRNISLVMTEADKALREAKSEGGNKVIIHHI"
BASE COUNT         2192 a         1981 c         1914 g         2186 t
ORIGIN      
        1 cttcggtcgc tctttttttt acctgataaa atgaagttaa aggactgcgt catgattaag
       61 aaaatttttg cccttccggt catcgaacaa atctcccctg tcctctcccg tcgtaaactg
      121 gatgaactgg acctcattgt ggtcgatcat ccccaagtaa aagcctcttt tgcattacag
      181 ggcgcacacc ttctctcgtg gaaacctgcg ggtgaagaag aagttctgtg gttgagcaac
      241 aacacaccgt tcaaaaatgg cgtcgctatt cgcggtggcg taccggtttg ctggccgtgg
      301 tttggtccgg cggcacaaca aggtctgcct gcgcacggtt ttgcccgcaa cctgccgtgg
      361 acgctgaaat cacataatga agatgctgat ggcgtagcgc tgacttttga attgacgcaa
      421 agcgaagaga cgaaaaaatt ctgtccgcac gactttacgc tgttagcgca tttccgcgtg
      481 ggtaaaacct gtgaaatcga tcttgaatca catggcgaat ttgaaaccac ctctgccctg
      541 catacctact ttaacgtggg tgatatcgct aaggtaagcg tcagtgggct gggcgatcgc
      601 ttcattgata aagtgaatga cgcgaaagaa gatgtactga ccgacggtat tcagaccttc
      661 cctgaccgta ccgatcgcgt gtatctgaat ccgcaagatt gcagcgtgat taatgatgaa
      721 gcgctgaatc gtattatcgc cgtaggccac cagcatcatc tgaacgttgt aggctggaac
      781 ccgggaccgg cgctttcaat tagcatgggc gatatgccgg atgatggcta caaaacattt
      841 gtttgtgtag aaacggctta cgcttcagaa acgcaaaaag tcaccaaaga gaaacctgca
      901 catctggcgc aatccattcg cgttgcgaaa cgttaatttg cgttaatgtt gtgtgccggg
      961 tgcgatgcat ccggcacgta tcaacatcac accatatcca gcgcagtttt tccttttggt
     1021 gccggatacg ctttatccag catggttaat tccgctgacg aaagttcgac ctcaagcaca
     1081 gccgcattct gttgaacatg ggcaatcgtg gccgcttttg gaatcgccat cacaccctga
     1141 tggctgatta cccacgccaa caatacttgt gccgagctga tattgtgagc atgtgcaatt
     1201 tcgttgacta ccgcgttttt taacagtcca ttgcgcaacc gcccggcctg ggctaatgga
     1261 ctgtaagcca tcaccggcat ctgctgttgc tggcaccagg ggagtagatc gtactcaatc
     1321 cctcgtgaac cgagatggta aagcacctga ttagtggcac actgatttcc ccccggcagc
     1381 tgccagagtt cctgcatatc agcataatca aggttagaaa cgccccagcg gcggattttt
     1441 ccctgggcga tcaatttttc catcgctgca acagtctctt caaaagcgaa actgccagac
     1501 ctgtgtaata agtagagatc gagataatca gtattgagac ggcgtaaact ggcttcgcat
     1561 gcatttatcg ctttttgccc gccagcattc cacggataga ctttagagac gagaaagacg
     1621 ttatcacgca gaccggttaa tgcttcccca accacctttt cggcaccgcc atcggcatac
     1681 atttcggcgg tatcaatgag ggttaaaccg agctcaatgc ccgcgcgtag tgcagcaact
     1741 tctgttttgc gctgactggc atcttcgccc atataccatg ttccctgccc tatggctggc
     1801 agtgagacat cgccactaaa ttgaatcatt ttttgttgca ttgtttcctc ccaggtattg
     1861 caccaccgta atgcaaaaca gggcgtgacg ccctgttttt atgcacaaaa tgccctggaa
     1921 agatgcatta tcagaatttg taggtgatcc cggtagaaat caggccagtc caggatttat
     1981 ccaccatcgg gctgtcagta acttcatcag acagacgggt atagcgcgcg gtaccgtaaa
     2041 cactccagtc gccgaggaag ttgtagctgg cgctcagctc caggtaaggg ctccagctgt
     2101 cgttcgggtt atagccacgc agaccgctgc gagcggactc tttgcgcgat acgccataat
     2161 agtattcgtt ctggttttcg ctgttccact gcacaccgat acccggggtc actgtcaggc
     2221 cgccgttggt gtaacgatac aaccaggcca tatcccagac aatgccgttg ctgttatcca
     2281 gggtatcgcc agtcagggtg gtacgcaggt aaccgtactg ggtaaagtga gcataagaca
     2341 gaccagccat catggtgctc ttacggtcat ccaggtgacg catttggtga tcgccactgt
     2401 ctttcgcttt gaagtaaagc ggcgaccaat aagcggtaat tgaaagtttg tccgttgcgt
     2461 cattccacag gtagtaacca ccacctaagc cacggaacca gaagttatcg ccttcatagt
     2521 tgattaccgg tactgggtaa acatcggtat cgtaatcttt atatgggtgt tcaacgacac
     2581 cgacgcctgc gcccagggaa aatttacctt cagcgtgcgc tacgcctgca gacgttgcga
     2641 taagcactcc aagtgccaga agtttgagtt tggtcacaat taatcattcc ttaaacaaat
     2701 gtttagcggg cgacaaagtt tacccgtcaa tacatcgaaa cccaaccttt ttacgttttc
     2761 attttttaaa gtaactgttt aattttcctg acgcggatga caccgcgctt acagccaaat
     2821 gaatttagcg ttactggcga gccttgtctt tacattaatt atgcaaaatt tatggatgag
     2881 ttgttgatat gccattgaaa ttaagaaagc cgtgcaggca agttttccat ttgccatcta
     2941 cgcttaattt tgaaggtgta tcaccgggca cgttgttctc atcgtcgata aaatggcatg
     3001 agagttgctg tgttttagca agagacgtcg ttcagtttac ctcttccggg agcctctact
     3061 attcatatga acggctctta acctgtgcta aaaaacgaaa ggacggcata ccatgaatat
     3121 attcgatcac tatcgccagc gatatgaagc tgccaaggac gaagagttca cactgcagga
     3181 gtttcttacc acttgtcggc aagatcgcag tgcttatgcc aacgcggctg agcggctatt
     3241 gatggctatc ggtgagcctg tcatggtcga tacagcccag gaacccagac tttctcgact
     3301 cttttctaac cgggtcattg cacgttatcc ggcatttgaa gagttttacg gcatggaaga
     3361 cgcgattgaa cagattgtct cttatctgaa acacgcggct caggggctgg aagagaagaa
     3421 acaaatcctg tatctgctgg ggcctgtggg tgggggtaaa tcatcgcttg ctgagcgact
     3481 gaaatcatta atgcagctcg taccgattta tgtattgagc gccaacggtg agcgtagccc
     3541 ggtcaacgat catccgttct gtcttttcaa tccgcaggaa gatgcgcaga ttctggaaaa
     3601 agagtatggc attcctcgcc gctatctcgg caccatcatg tcgccgtggg cggcaaaacg
     3661 cctgcatgaa tttggtggcg atatcactaa gttccgggta gtgaaggtct ggccgtcaat
     3721 tctgcaacaa attgctatcg ccaaaacaga acccggtgat gagaacaacc aggacatctc
     3781 cgcgctggta gggaaagtcg atattcgtaa actcgaacac tacgcgcaga atgacccgga
     3841 cgcctacggc tattccggtg cgctgtgccg cgccaatcag gggatcatgg aatttgtcga
     3901 gatgtttaaa gcaccgatta aagtgctgca tccgctgtta accgctactc aggaaggtaa
     3961 ctacaacggg acggaaggta tctccgccct gccgttcaac gggattattc tcgcccactc
     4021 gaacgaatcc gaatgggtca ctttccgtaa taacaaaaac aacgaagcct tcctcgaccg
     4081 tgtttacatc gtgaaggtgc cgtattgctt acgcatttcc gaagagatca aaatctacga
     4141 gaaattgctt aatcacagtg aattgactca cgccccatgc gcccctggca cgctggaaac
     4201 actgtcacgt ttttccattc tttcgcgcct gaaagagcca gaaaactcca gcatttattc
     4261 aaagatgcgg gtttatgatg gcgaaagtct gaaagacacc gatcccaaag ccaagtcgta
     4321 tcaggaatat cgtgactacg ccggtgtcga tgaagggatg aaaggtctgt cgacgcgttt
     4381 tgcgtttaag atcctctccc gcgtgttcaa cttcgatcat gtagaagtgg cggcaaaccc
     4441 ggtccatctg ttctacgtcc tagaacagca gatcgagcgc gagcagttcc cgcaagagca
     4501 ggcagaacgc tatctggagt tcctgaaagg ttatctgatc ccgaaatatg ccgagtttat
     4561 cggcaaagag atccagacgg cctaccttga atcctattcc gaatatgggc aaaacatttt
     4621 cgaccgttat gttacctacg cggatttctg gattcaggat caggagtatc gcgatccgga
     4681 taccgggcag ctgtttgacc gcgagtctct taacgccgag ctggagaaaa tcgagaaacc
     4741 ggcggggatc agtaatccaa aagatttccg caacgagatt gttaacttcg tactgcgcgc
     4801 cagagcgaat aacagcggac gcaatccgaa ctggaccagc tatgaaaaac tgcgcacggt
     4861 tatcgagaag aaaatgttct ccaataccga ggagctgttg ccggttatct cgtttaacgc
     4921 caaaacgtca accgacgagc agaagaaaca cgacgacttt gtcgaccgta tgatggaaaa
     4981 aggctacacc cgtaaacagg tgcgtttact gtgcgaatgg tatttgcgcg tacgtaaatc
     5041 gtcttaacaa ccctggcccg gtacacatgc taccgggcct ataacgacag cgaaccgtgg
     5101 gccagagaaa tggcaacaca ggcgtagcat acagttggca aatgtagtac ggggggcata
     5161 tgaccgggtt tattgaccgg cgtctgaacg gcaaaaacaa aagcatggtg aatcgccagc
     5221 gttttttacg ccgttataaa gcgcaaatta aacagtcgat ctccgaggcc attaataagc
     5281 gttcggtgac tgacgtcgac agcggcgaat ccgtatccat tcccacggaa gatattagcg
     5341 aaccgatgtt tcatcagggg cgtggcggcc tgcgccaccg cgtacatccg ggcaatgacc
     5401 atttcgtcca gaacgaccga attgaacgtc cccagggtgg cggcggaggt tccggcagtg
     5461 gtcagggcca ggccagccag gatggtgaag gtcaggatga atttgtcttt cagatttcga
     5521 aagatgagta tcttgatctg ctctttgaag atttggcctt accgaatctg aaacaaaacc
     5581 aacaacgcca gctgaccgaa tataaaacgc atcgggcggg ttataccgct aacggcgttc
     5641 cagccaatat cagcgttgtg cgttccttgc agaactcact ggcgcgacgc acagccatga
     5701 cggcgggcaa gcgacgggaa cttcatgcgc tggaagagaa tttggccatc atcagcaaca
     5761 gtgaacctgc gcaactgctg gaagaggaac gtctgcgcaa agaaattgca gaattacgtg
     5821 ccaaaattga acgcgtccct tttattgaca ccttcgattt acgttacaag aactacgaga
     5881 agcggcccga tccctccagc caggcagtga tgttttgcct gatggacgtt tccggttcaa
     5941 tggatcaatc cactaaagat atggctaagc gtttttatat tctgctgtat ctgttcctca
     6001 gcagaacgta taagaacgtg gaagtcgtat acatccgcca tcatacccag gcgaaagaag
     6061 tcgatgaaca tgagtttttc tactcgcagg aaacaggcgg caccattgtt tccagcgccc
     6121 tgaaactgat ggatgaggta gtgaaagagc gttataaccc ggcacagtgg aatatttacg
     6181 ctgcacaagc atcggacggc gataactggg ccgatgactc tccgctttgc catgaaatcc
     6241 tggcgaaaaa attattacct gttgttcgtt attacagcta tatcgaaatt acccgtcgtg
     6301 cacatcagac attgtggcga gaatatgagc atctgcaatc tactttcgac aactttgcga
     6361 tgcagcacat ccgcgaccag gatgatattt atccggtgtt ccgtgaactg tttcataaac
     6421 aaaatgcaac agctaaagac taaaactatc agccaggtca ttatcgcctg gctgattttt
     6481 agcttactgt aaattatctc atttattaca tacattagct tacaatcgct ttaaatatga
     6541 cagcataacc tttacataat ttagttccag aaaacaatca ttcggaaaaa tgattcagtc
     6601 aacacgtatt tccatggggt tattctttaa atatttttta tcgttaacga aaattgatcc
     6661 tggtcaaaac tatatatctc tgccatcaat aaaatccagc actcacattg ctctcctttt
     6721 tatggtttct atgggtacac aaaaattaaa agctcaaagc ttttttattt tcagtttatt
     6781 gctgacgtta attttatttt gcattactac cttatataac gaaaacacaa atgtaaaact
     6841 catcccacag atgaattacc tgatggttgt tgtggctttg tttttcctta acgccgtcat
     6901 ttttcttttc atgttaatga aatatttcac taacaaacaa attttaccaa cactcatttt
     6961 aagccttgca tttttaagtg gccttatcta tttagttgaa accattgtaa ttatccataa
     7021 accaattaac ggcagtacac tgatccagac aaagtcgaat gatgtttcta ttttctatat
     7081 tttccgccaa ctcagtttta tttgtttaac ctcgctggca ctcttttgtt atggaaaaga
     7141 caacatcctt gacaacaata agaaaaaaac gggaatcctg ttgctggcgc tgattccttt
     7201 tttagttttt ccccttctgg cacacaatct gagcagttat aacgctgact attctttgta
     7261 tgtcgtcgac tactgtccgg acaaccatac tgcgacctgg ggaatcaact atacaaaaat
     7321 attggtttgt ctgtgggcat ttttactgtt ctttattatc atgcgtacac gattagccag
     7381 cgaactatgg ccgttaatag cattattatg tctggcatcg ctatgctgca acttacttct
     7441 actgactctg gatgagtata attacaccat ctggtatatc agtcgcggga ttgaagtttc
     7501 cagtaaactg tttgttgtgt cttttctgat ttataacatt tttcagaaac tgcaactctc
     7561 cagcaaactg gcagttcatg atgtgctgac caatatttat aatcggcgct actttttcaa
     7621 cagcgtagag tcattattgt cgagacctgt tgttaaggac ttctgtgtca tgctggttga
     7681 tattaatcag ttcaaacgca tcaatgccca atggggacat cgtgtgggtg ataaagtgct
     7741 ggtttcaatt gtcgatatta tccagcaaag catccgcccc gatgatattt tagcgcgact
     7801 ggagggtgag gtgtttggct tgctatttac cgaactcaat agtgcccagg caaaaatcat
     7861 tgcggaacgt atgcgtaaaa atgtcgaact cctgaccggc tttagtaaca gatatgatgt
     7921 tcctgaacaa atgaccatca gtattggcac ggttttttca acgggtgaca cgcgtaatat
     7981 ctcgcttgtc atgacggaag cagataaagc cttacgcgaa gcgaaaagcg aggggggcaa
     8041 caaagtgatt attcatcata tttaagtgca aaaatattca gagccatgct ttttgcgtgg
     8101 cttttgcata caatttatta ctattacccc taaatttccc ctccccagcg gcgcagagat
     8161 gagtataatt agcgcccctg tgccaggccg caatcgaact ttatctggtt ttctcgtttc
     8221 actaaccgaa ggagtgccat ttatcatgaa attgcaccat agaatgctcc ggc
//
LOCUS       DADMKF010000164         8237 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.167, whole genome shotgun sequence.
ACCESSION   DADMKF010000164 DADMKF010000000
VERSION     DADMKF010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8237
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.167"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            44..568
                     /gene="radC"
                     /locus_tag="JNC24_003460"
     CDS             44..568
                     /gene="radC"
                     /locus_tag="JNC24_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000794253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HAY9160129.1"
                     /translation="MNKLESLPGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYN
                     VRMREESPLLSPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEV
                     HPREIIREAIKINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVI
                     GRGEYVSFAERGWI"
     gene            785..1021
                     /gene="rpmB"
                     /locus_tag="JNC24_003461"
     CDS             785..1021
                     /gene="rpmB"
                     /locus_tag="JNC24_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HAY9160130.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            1042..1209
                     /gene="rpmG"
                     /locus_tag="JNC24_003462"
     CDS             1042..1209
                     /gene="rpmG"
                     /locus_tag="JNC24_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAY9160131.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            1307..2116
                     /gene="mutM"
                     /locus_tag="JNC24_003463"
     CDS             1307..2116
                     /gene="mutM"
                     /locus_tag="JNC24_003463"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HAY9160132.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(2155..2634)
                     /gene="coaD"
                     /locus_tag="JNC24_003464"
     CDS             complement(2155..2634)
                     /gene="coaD"
                     /locus_tag="JNC24_003464"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HAY9160133.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALTQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALVAKLA"
     gene            complement(2642..3919)
                     /gene="waaA"
                     /locus_tag="JNC24_003465"
     CDS             complement(2642..3919)
                     /gene="waaA"
                     /locus_tag="JNC24_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid transferase"
                     /protein_id="HAY9160134.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDADYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            4332..5390
                     /gene="rfaQ"
                     /locus_tag="JNC24_003466"
     CDS             4332..5390
                     /gene="rfaQ"
                     /locus_tag="JNC24_003466"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HAY9160135.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTTPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGLDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            5387..6511
                     /locus_tag="JNC24_003467"
     CDS             5387..6511
                     /locus_tag="JNC24_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAY9160136.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYHLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            6504..7301
                     /gene="rfaP"
                     /locus_tag="JNC24_003468"
     CDS             6504..7301
                     /gene="rfaP"
                     /locus_tag="JNC24_003468"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HAY9160137.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGI
                     NPLSRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAVGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPRRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFVAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
     gene            7317..>8237
                     /gene="waaO"
                     /locus_tag="JNC24_003469"
                     /gene_synonym="rfaI"
     CDS             7317..>8237
                     /gene="waaO"
                     /locus_tag="JNC24_003469"
                     /gene_synonym="rfaI"
                     /EC_number="2.4.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     3-alpha-galactosyltransferase"
                     /protein_id="HAY9160138.1"
                     /translation="MSAHYFNPQEMINKTIIFDERPAASVASSFHVAYGIDKNFLFGC
                     GVSITSVLLHNNDVSFVFHVFIDDIPEADIQRLAQLAKSYRTCIQIHLVNCERLKALP
                     TTKNWSIAMYFRFVIADYFIDQQDKILYLDADIACQGNLKPLITMDLANNVAAVVTER
                     DANWWSLRGQSLQCNELEKGYFNSGVLLINTLAWAQESVSAKAMSMLADKAVVSRLTY
                     MDQDILNLILSGKVKFIDAKYNTQFSLNYELKKSFVCPINDETVLIHYVGPTKPWHYW
                     AGYPSAQPFINAKEASPWKNEPFMRPINSNY"
BASE COUNT         2122 a         1896 c         2078 g         2141 t
ORIGIN      
        1 ctgcatgaca aagtcatcgg gcattatctg aacataaaac actatcaata agttggagtc
       61 attacctggc ttgttaacct ctgaatatga acaatttagt ggcgttcatg gaattggcgt
      121 agcgaaattt gcccagttaa aggggattgc tgaactggcg cggcgttact acaacgtgcg
      181 gatgcgtgaa gaaagccctt tactcagccc ggagatgacg cgggaatttt tacaaagcca
      241 gctcacgggt gaggagcggg agatctttat ggtgatcttt ctcgactcac aacaccgggt
      301 tataacgcat agccgtcttt tttccggcac gctaaaccat gttgaagtcc atcctcggga
      361 aattatccgg gaagcgataa aaataaacgc ctcggcgctg atccttgcac ataatcaccc
      421 ttcgggttgt gctgaaccca gtaaagcgga taaactcatt actgaacgga taataaagag
      481 ttgtcagttc atggatttac gcgtgctcga ccatatcgtg attgggcgtg gagagtatgt
      541 ttcttttgcc gaacgcggct ggatttaacc cgctatgcgc gatccttcgg gatctttgtc
      601 tgttcgggac ttgagcacat cgctgagtca gcgtatacta cgccaccttt gagaatctcg
      661 ggtttggcat ttgggcctgg caatcgagag ttcacataga actgcgatga ccgggctgta
      721 aagcctgacg aggcgccaat accccatacg aagctcgagc taatttgatt tttggagaat
      781 agacatgtcc cgagtctgcc aagttactgg caagcgtccg gtgaccggta acaaccgttc
      841 ccacgcactg aacgcgacta aacgccgttt cctgccgaac ctgcactctc accgtttctg
      901 ggttgagagc gaaaagcgtt ttgtcaccct gcgcgtatct gctaaaggta tgcgtgtaat
      961 cgataaaaaa ggcatcgata cagttctggc tgaactgcgt gcccgtggcg aaaagtacta
     1021 agtacttaga ggaaataaat catggctaaa ggtattcgtg agaaaatcaa gctggtttct
     1081 tctgctggta ctggtcactt ctataccact acgaagaaca aacgtactaa gccggaaaaa
     1141 ctggaactga aaaaattcga tccagttgtt cgccagcacg tgatctacaa agaagcgaaa
     1201 atcaaataat tctcgctttg atgtaacaaa aaacctcgct ccggcggggt tttttgttat
     1261 ctgcttgccc ccatattgat tgcatctgtt cattcctgga gatgctatgc ctgaattacc
     1321 cgaagttgaa accagccgtc gcggcataga accgcatctc gttggtgcaa ccattcttca
     1381 tgcggtggtg cgcaacggac gcttgcgctg gccggtttca gaagagatct accgtttaag
     1441 cgaccaacca gtgcttagcg tgcagcggcg ggctaaatat ctgctgctgg agctgcctga
     1501 gggctggatt atcattcatt tggggatgtc tggcagcctg cgcatccttc cagaagaact
     1561 tccccctgaa aagcatgacc atgtggattt ggtgatgagc aacggcaaag tgctgcgcta
     1621 caccgatccg cgccgctttg gtgcctggct gtggaccaaa gagctggaag ggcacaacgt
     1681 gctggctcat cttggaccgg agccgcttag cgacgatttc aatggtgagt atctgcatca
     1741 gaagtgcgcg aagaaaaaaa cggcgattaa accgtggctg atggataaca agctggtggt
     1801 aggggtaggg aatatctatg ccagcgaatc actgtttgcg gcggggatcc atccggatcg
     1861 gctggcgtca tcactgtcgc tggcagagtg tgaattgtta gctcgggtga ttaaagcggt
     1921 gttgctgcgt tcgattgagc agggtggtac aacgctgaaa gattttctgc aaagtgatgg
     1981 taaaccgggc tatttcgcgc aggaattgca ggtttacgga cgaaaaggtg agccgtgtcg
     2041 ggtgtgcggt acgccgattg tggcgactaa acatgcgcag cgggcaacgt tttattgtcg
     2101 gcagtgccag aagtaattca tacgcgccgg atggcatacc atccggcata aacgctacgc
     2161 taacttcgcc accagcgcct gatggacatt ctccggcagg aaatgggtga catcgccctg
     2221 atggcgcgcc acctctttca ccaacgatga agagataaac gaccactctt tcgacggcat
     2281 cagaaacaca ctttccagtt ccggcattaa gtggcgattc atatgcgcca gctgcatttc
     2341 atattcaaaa tccgccaccg cacgcaggcc acgaatcagc accgtagcgt gttgattacg
     2401 ggcgaagttc gccatcaaat cactaaaccc aaccacttcc acgttcccca gatgcgcggt
     2461 tgcctgctgt gtcagcgcca cacgctcttc cagggtaaac atcggttttt tactggggct
     2521 ggcggcaatc gccagaataa cgtgatcgaa catttgcgtg gcgcgcgtca cgatatcgat
     2581 atgaccattg gtaatgggat cgaaagtacc cggataaatc gcccgttttt gcataacaac
     2641 ctcaatgcgt tttcggtggc aggtaaggtt ccagcagttg aagcagacgc tgtagcgcgc
     2701 cctggttttg atacagtact tcaacggcat gacggccata gaaactacgg taatcggcgt
     2761 cggtgagtaa agaggaaacc tcttttgcaa gcgtagtggc atcggtaacg gtaatcagcc
     2821 cgcttgcctg ctccagccgc gcgcaaatgt ctttgaaatt aaaagtatgc ggccccatca
     2881 ataccggaat agcgtgtgcg gcagcttcca gaggattatg ccctccacgt tcaaccagtg
     2941 aaccgccaac aaaggcgaga tcggcaatgc cgtacagtaa catcaactcg cccatcgtat
     3001 cgccaaccac aacctgcgtg ctggtggagg ggacttcccc tgaagagcgt gtgatatagc
     3061 ttagtccagc ctggcggaca aggttaatcg catccgggaa gcgttctgga tgacggggta
     3121 ccaggatgag caataaattc gggaattgct gtaataatgc ctgatgtgcg gcgatcacca
     3181 cactctcttc accttcgtga gtgctggtgg caatccatac cgggcggtgt ggtgcccact
     3241 ggcggcgcag cgtcacggct ttagcagcca actgcggcgt tacagaaata tcgaatttca
     3301 ggctaccggt aacggttacc tggttatttt ttgcgcccag cgccacaaaa cgtgcaccat
     3361 cttcttcatt ttgcgcagca atcagcgtaa tacgacgcag taagcggcgg acgaatttac
     3421 ccagtttggc ataacctgcg gccgagcggg cagagagtcg cgcgttagcg atcaccagcg
     3481 gaattttacg tttatgtagc gccgcaatca ggttaggcca tagttcggtt tccataatca
     3541 acaccagttt ggggtcgact ttattcagga aacggttgag cgcatcgggc aggtcatacg
     3601 gcagataaac gtgctgaaca tccttcccga aagccgattg tacgcgctcc gaaccggttg
     3661 gcgtcatggt tgtaacggta atcggtaaat caggataacg atggcgcagc gcgcgcacca
     3721 acgggatcgc cgccagagtt tcaccgacgg agacggagtg cagcataatg ccgcctggtt
     3781 ttagcggatg gcggtaaaaa ccgtaacgtt caccccagcg ttttcgatag gccggagcct
     3841 tacgtccgcg cacccagagc cgtatccaga tcagcggctg aataaggtag agaagggcgg
     3901 tgtaaagcaa ttcgagcata gtaaatagct gacttatgga tgtgctgggg attctatgta
     3961 tttagctgtg gctttaccat tacttttccc gtttttgact taaatagcaa tttacccgcc
     4021 aattcttgct gaacatctat actttttttg tatttttatg cgattcattg atttctgctg
     4081 tacgtgcagg aaggaatcgc agagcaacag tgaaagaata tggttgctaa tgattttatt
     4141 tgattgattt ctttattgaa aatcaacaat gtgcttaaaa agcattcggg tcggattgcc
     4201 gcgaaagaag tgatacacta gcgcgtcaat atcagtgcgg tatcagtatt caggtagctg
     4261 ttgagcctgg ggcggtagcg tgctttttct gcttcacctt aatcggataa tctcaacaaa
     4321 aagagttact tgtggataag ccatttcgaa gaattttgct cattaagatg cgttttcatg
     4381 gggatatgtt attaactact cccgtcatta gttcgctgaa aaaaaattac cctgacgcaa
     4441 aaatcgatgt gctgctttat caggacacca ccccgatcct gtctgaaaat ccagagatta
     4501 acgcgctcta cggcataaaa aataaaaaag caaaagcctc agaaaaaatt gccaactttt
     4561 ttcatctcat caaggtatta cgtgccaata agtatgacct tatcgtcaat cttaccgatc
     4621 aatggatggt tgctatactg gttcgcttat taaatgcccg tgtgaaaatt tcccaggatt
     4681 atcatcatcg gcagtctgct ttttggcgta aaagtttcac ccatttagtg ccgttgcagg
     4741 gtggaaatgt ggtggaaagt aacttatccg tgctgacacc attgggactt gattcgttgg
     4801 tgaagcagac aaccatgagt tacccgcctg caagctggaa acgtatgcgt cgcgaacttg
     4861 atcacgctgg tgttggacaa aattatgtgg ttatccaacc tacggcgcgg caaatcttca
     4921 aatgctggga caacgccaag ttttccgctg tgattgatgc cttacatgct cgtggttatg
     4981 aagtcgttct gacgtccggc ccagataaag acgatctggc ctgcgtcaat gaaattgcgc
     5041 agggatgcca gacgccacca gtaacggcgc tggctggaaa ggtgaccttc ccggaacttg
     5101 gtgcgttaat cgatcatgcg cagctgttta ttggcgttga ttccgcaccg gcgcatattg
     5161 ccgctgcagt taatacgccg ctgatatcgc tgtttggtgc gacagaccat attttctggc
     5221 gtccttggtc aaataacatg attcaattct gggcgggaga ttaccgggaa atgccaacgc
     5281 gcgatcagcg tgaccgaaat gagatgtatc tttcggttat tccggcggca gatgtcattg
     5341 ctgctgtcga taaattactg ccctcctcca cgacaggtac gtcgttatga tcgttgcttt
     5401 ttgtttatat aaatattttc cctttggcgg tttgcagcgc gattttatgc gtattgctca
     5461 gacagtcgcc gcccgaggtc atcatgttcg ggtttatacc cagtcgtggg aaggcgaatg
     5521 ccctgatgta tttgaactga tcaaagtgcc ggttaaatcg cataccaatc acgggcgcaa
     5581 tgcggagtat tttgcctggg tgcaaaaaca tttacgcgaa catcccgtcg ataaagtcgt
     5641 tggattcaac aaaatgccgg ggctggacgt ttattatgcc gctgatgttt gttatgccga
     5701 gaaagtcgcg caggaaaaag gctttttcta tcacctgacg tcacgttatc gccattatgc
     5761 cgcctttgag cgggcaacct tcgaacaggg caagccgaca cagctgctga tgctgacaga
     5821 taagcaaatc gccgatttcc agaaacatta tcagactgaa gcggagcgtt ttcatattct
     5881 gccaccgggg atttatcctg atcgtaaata tagccagcag ccagctaata gccgtgaaat
     5941 cttccgtaag aagaatggaa taaccgaaca acaatattta ttgttgcagg tcggttcaga
     6001 cttcacgcgt aaaggtgtcg atcgttccat tgaagcactt gcttcgttac cggattcgct
     6061 gcgccacaac acattgctat atgttgttgg acaggataag ccgcgaaaat ttgaggcgct
     6121 ggcagaaaaa cgcggcgtgc gcagtaatgt tcacttcttc tcggggcgca acgatgtctc
     6181 ggaattaatg gcggcggcgg atttattact gcatcctgcc taccaggaag cagcgggaat
     6241 tgtgctactg gaagcgatta ctgcaggatt accggtacta acaacagccg tttgtggcta
     6301 tgcgcattat attgtcgacg ccaattgcgg cgaggctatt gctgagccat tccgccagga
     6361 aacattgaat gagattttac gcaaagcgtt aacgcaatct tcattgcgcc aggcctgggc
     6421 ggaaaatgcg cgacattatg ctgatacaca agatttatac agtctgccag agaaagcggc
     6481 ggatatcata acgggtggtc tggatggttg aacttaaaga gccgtttgcc acgttatggc
     6541 gcggtaaaga tccttttgag gaagttaaaa ccttgcaggg tgaggttttt cgtgaactgg
     6601 aaactcgccg cactctgcga tttgaaatgg cgggcaaaag ctattttctc aaatggcatc
     6661 gcggcacgac cctgaaagag ataatcaaaa atttactctc attgcggatg ccagtattag
     6721 gcgcagaccg cgaatggaat gcgattcatc gactgcggga tgtcggcgtt gatactatgt
     6781 atggggtggc atttggcgaa aaaggtatta atccgctgag cagaacctcg tttattatta
     6841 ccgaagatct gaccccaacc atcagtctgg aagattactg tgctgactgg gcgaccaacc
     6901 caccagatgt tcgcgtaaag cgtatgctta ttaagcgtgt cgcgacgatg gtgcgcgata
     6961 tgcatgctgt gggcattaac caccgtgact gttatatctg tcatttcctg ctgcacttgc
     7021 ctttttccgg taaggaagag gagttaaaaa tttcggtaat tgacctgcac cgggcgcagc
     7081 ttcgcacgcg cgttccacgt cgttggcgcg ataaagatct tattgggctt tatttttctt
     7141 cgatgaatat cggcctgact cagcgggata tctggcggtt tatgaaagtg tattttgtcg
     7201 ccccgcttaa agacattctc aagcaggaac aaggactgct gtcgcaagca gaagcaaaag
     7261 ccacaaaaat cagggaaaga acgattcgaa aatcgttgta atttccggag agaaatatga
     7321 gtgcccacta ttttaatcca caagagatga tcaataagac aatcatcttc gatgaaaggc
     7381 cagcggcgtc agtggcatca tcattccatg ttgcttatgg cattgataaa aactttcttt
     7441 ttggttgtgg tgtttcaatc acgtcagttt tgttacataa caacgacgtg agttttgttt
     7501 tccacgtttt tattgatgat atccctgaag ccgatatcca gcgtttagcc caattggcga
     7561 aaagctatcg tacctgtatc cagatccatc tggtaaattg tgaacggctt aaggcattac
     7621 cgacgaccaa aaattggtct attgccatgt atttccgttt tgtaattgca gattacttta
     7681 ttgatcaaca agataagatc ctttacctgg atgctgatat cgcctgtcag ggaaacttaa
     7741 agccgctgat aacaatggat cttgccaata acgttgctgc tgttgttact gaacgcgatg
     7801 ctaactggtg gtcgttacgg ggtcaaagtc tgcagtgtaa tgaacttgaa aagggttact
     7861 ttaattcagg tgtcctgtta attaatacac tagcgtgggc gcaggagtcc gtttctgcta
     7921 aagcgatgtc gatgcttgct gataaagccg tcgtttcccg tttaacctat atggatcaag
     7981 atatacttaa tcttatcctg tcagggaaag ttaaattcat tgatgctaaa tacaatactc
     8041 aatttagttt aaattatgaa ttaaaaaaat catttgtttg tccaattaat gatgaaaccg
     8101 tattaattca ttatgtcggc ccgacaaaac cctggcatta ctgggccggt tatccaagtg
     8161 cgcaaccttt tatcaacgcc aaagaagcat cgccctggaa aaatgaaccg tttatgcggc
     8221 caattaattc aaactat
//
LOCUS       DADMKF010000165         8217 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.168, whole genome shotgun sequence.
ACCESSION   DADMKF010000165 DADMKF010000000
VERSION     DADMKF010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8217
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.168"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(218..1108)
                     /gene="dgcZ"
                     /locus_tag="JNC24_003470"
     CDS             complement(218..1108)
                     /gene="dgcZ"
                     /locus_tag="JNC24_003470"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcZ"
                     /protein_id="HAY9160139.1"
                     /translation="MIKKTTEIDAILLNLNKAIDAHYQWLVSMFHSVVARDASKPEIT
                     DNHSYGLCQFGRWIDHLGPLDNDELPYVRLMDSAHQHMHNCGRELMLAIVENHWQDAH
                     FDAFQEGLLSFTAALTDYKIYLLTIRSNMDVLTGLPGRRVLDESFDHQLRNTDPLNLY
                     LMLLDIDRFKLVNDTYGHLIGDVVLRTLATYLASWTRDYETVYRYGGEEFIIIVKAAN
                     DEEACRAGVRICQLVDNHAITHSEGHINITVTAGVSRAFPEEPLDVVIGRADRAMYEG
                     KQTGRNRCMFIDEQNVINRV"
     gene            complement(1363..1755)
                     /gene="ydeI"
                     /locus_tag="JNC24_003471"
     CDS             complement(1363..1755)
                     /gene="ydeI"
                     /locus_tag="JNC24_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
                     /protein_id="HAY9160140.1"
                     /translation="MKFQSIVLASFLVMPYALADDQGGLKQDAAPPPPHAIEDGYRGT
                     DDAKKMTVDFAKNMHDGASVSLRGNLISHKGEDRYVFRDKSGEINVVIPAAVFDGREV
                     QPDQMINISGSLDKKSAPAVVRVTHLQK"
     gene            2031..2549
                     /locus_tag="JNC24_003472"
     CDS             2031..2549
                     /locus_tag="JNC24_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HAY9160141.1"
                     /translation="MNINKDKIVQLADTDTIENLTSALSQRLIADQLRLTTAESCTGG
                     KLASALCAAEDTPKFYGAGFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAI
                     ERADADVSIAITGYGGPEGGEDGTPAGTVWFAWHIKGQNYTAVMHFAGDCETVLALAV
                     RFALAQLLQLLL"
     gene            complement(2594..4639)
                     /gene="dcp"
                     /locus_tag="JNC24_003473"
     CDS             complement(2594..4639)
                     /gene="dcp"
                     /locus_tag="JNC24_003473"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HAY9160142.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELLTRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDGNDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGQRFREAILSRGNSEDLERLHRQWRGKAPQIMPMLQHRGLNI"
     gene            4776..5522
                     /gene="ydfG"
                     /locus_tag="JNC24_003474"
     CDS             4776..5522
                     /gene="ydfG"
                     /locus_tag="JNC24_003474"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HAY9160143.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWCNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            5611..6297
                     /locus_tag="JNC24_003475"
     CDS             5611..6297
                     /locus_tag="JNC24_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAY9160144.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            6474..6677
                     /gene="ydfZ"
                     /locus_tag="JNC24_003476"
     CDS             6474..6677
                     /gene="ydfZ"
                     /locus_tag="JNC24_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HAY9160145.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(6713..8173)
                     /locus_tag="JNC24_003477"
     CDS             complement(6713..8173)
                     /locus_tag="JNC24_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAY9160146.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIIHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQM
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLDHPMVAADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAKAVDVKLAQWIEDNVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVGDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRYAAYGLMLQEQAP
                     TLKVQGVDLQDYANRLIERYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GDDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
BASE COUNT         2173 a         2055 c         1871 g         2118 t
ORIGIN      
        1 gaacggagat aatccctcac ctaaccggcc ccttgttaca gttgtgtaca aggggcctga
       61 tttttatgac ggcgaaaaaa aaccgccagt aaaccggcgg tgaatgcttg catggataga
      121 tttgtgtttt gcttttacgc taacaggcat tttcctgcac tgataacgga tcgttgacac
      181 agtagcatca gttttctcaa tgaatgttaa acggagctta aactcggtta atcacatttt
      241 gttcgtcaat aaacatgcag cgatttcttc cggtttgctt accctcatac attgcccggt
      301 ctgctcttcc aatgaccaca tccagaggct cttcaggaaa tgcgcgactc acacctgctg
      361 tcacggtaat gttgatatgc ccttcagaat gtgtgatggc atggttatcg actaactggc
      421 aaattctgac acctgcacga catgcttctt catcattagc cgctttaaca ataatgataa
      481 attcttcgcc cccgtagcga taaaccgttt cgtaatcacg cgtccaactg gctaagtaag
      541 ttgccagggt gcgtaatact acatcgccga ttaaatgccc gtaggtatca ttaaccaatt
      601 taaatcggtc aatatccaac aacattaaat aaagattcag aggatcagtg ttgcgtaact
      661 gatgatcaaa ggattcatca agaacccgac gccccggcaa tcccgtcaaa acatccatat
      721 tgctacggat cgtcagcaaa taaattttgt aatcggttaa tgccgcagta aaagaaagca
      781 acccctcctg aaaggcgtcg aaatgcgcgt cctgccagtg attttcaaca atagccagca
      841 ttaattcccg accacagtta tgcatatgtt gatgggcaga atccattagc cgaacgtaag
      901 gtaattcatc gttatcgagt ggccccagat gatcaatcca ccgaccaaac tggcacagtc
      961 cataagaatg gttatccgtt atttctggct tactggcatc tctcgcgacc acgctgtgaa
     1021 acatactcac cagccactgg tagtgggcat cgatagcctt attgagattt aacaagatgg
     1081 catcaatttc cgttgtcttc ttgatcattg ccactccttt ttcacagttc cttgtgcgcg
     1141 ctattctaac gagagaaaag caaaattacg tcaatatttt catagaaatc cgaagttatg
     1201 agtcatctct gagataacat tgtgatttaa aacaaaatca gcggataaaa aagtgtttaa
     1261 ttctgtaaat cacctctgca ttatcgtaaa taaaaggatg acaaatagca taacccaata
     1321 ccctaatggc ccagtagttc aggccatcag gctaatttat ttttatttct gcaaatgagt
     1381 gacccgaacg acggccggcg cgcttttctt atccagactg ccgctaatgt tgatcatctg
     1441 gtccggctga acttctcgtc catcaaagac ggccgcagga ataacaacat taatttcacc
     1501 gctcttatcg cgaaaaacgt aacggtcctc tcctttgtga gaaatcaaat taccgcgtag
     1561 tgaaaccgaa gcgccatcgt gcatgttttt tgcgaaatca acggtcattt tttttgcatc
     1621 atcggttccg cgatagccat cttctattgc atgaggcggc ggtggcgctg catcctgttt
     1681 taaaccgccc tggtcatctg ccaacgcata aggcatgaca agaaaacttg ctaatacaat
     1741 ggactgaaat ttcatactaa ctccttaatt gcgtttggtt tgacttatta agtctggttg
     1801 ctatttttat aattgccaaa taagaatact gccaattgtt ataaggcatt taaaatcagc
     1861 ccactagctg tcaaatattc agagaatttg actcactaaa gttaagaaga ttgaagagtc
     1921 ttaaacatgt tttcagaata atcggattta tatgtttgaa aattattata ttggacgagc
     1981 atacagaaaa agcaaatcac ctttacatat aatagcgtgg acaaaaaaca gtgaacatta
     2041 ataaagataa aattgtacaa ctcgcagata ccgacactat tgaaaacctg acatccgcgt
     2101 tgagtcaaag acttatcgcg gatcaattac gcttaactac cgccgaatca tgcaccggcg
     2161 gtaagttggc tagcgccctg tgtgcagctg aagatacacc caaattttac ggtgcaggct
     2221 ttgttacttt caccgatcag gcaaagatga aaatcctcag cgtaagccag cagtctcttg
     2281 aacgatattc tgcggtgagt gagaaagtgg cagcagaaat ggcaacaggt gccatagagc
     2341 gtgcggatgc tgatgtcagt attgccatta ccggctacgg cggaccggag ggcggtgaag
     2401 atggtacgcc agcgggtacc gtctggttcg cgtggcatat taaaggccag aactacactg
     2461 cggttatgca ttttgctggc gactgcgaaa cggtattagc tttagcggtg aggtttgccc
     2521 tcgcccagct gctgcaatta ctgctataac caggctggcc tggcgatatc cccaggccag
     2581 ccattggtgg tgtttatatg ttcaagccac gatgttgcag catcggcata atctgaggag
     2641 ccttaccgcg ccattgtcga tgcaggcgtt ccagatcttc gctgttacct ctggaaagga
     2701 tcgcctcgcg aaaacgctgc ccattttcac gcgttaatcc gccctgctca acaaaccact
     2761 ggtaaccatc atcggccaac atttgcgtcc acagataagc gtaataacct gcagcatatc
     2821 cgccaccaaa aatatgggcg aaataactgc tgcgatagcg tggcggtata gcaggaagat
     2881 ccatattttc cgccaccagc gcccgcaatt caaaatcatc gacatcctgc attgcttcgt
     2941 tttcttccag gcaatgccag cgcatatcga gaagtgcggc gctaagcagt tcgctcatct
     3001 catacccttt gttgaacagg ctggcattac gcattttctg ttgcagttcg tcaggcattg
     3061 ctgccccgct ctgataatgc cgggcgtagc gagcgaatac ctgcggatgc gttgcccagt
     3121 gttcgttgat ttgcgacgga aattcgacaa aatcacgcgg cgtgttggtg ccggaaagcg
     3181 tggcataacg ctggcgggca aaaaggccgt gcagcgtatg accaaattca tggaataagg
     3241 ttattacatc atcccagagt aacaacgcag gctcaccggc agcgggtttc tgataattgc
     3301 agacgttata aattaccgga tgcgtttcat taagcgttga ttgctcaaca aaattgccca
     3361 tccatgcacc gccgcttttt gaatcacggg cgaagaaatc accgtaaaat aacgccagcc
     3421 ccacgccatt atgatcaaaa atttcccaca cccgaacgtc aggatggtag acaggaatat
     3481 caaaacgttc gacaaactta ataccgaaga gctgattcgc ggtccagaat acaccttcat
     3541 tcaacaccgt gtttaattca aaatatggct tgagctgcgc ttcatcaaga tcaaatttct
     3601 cccgccgtac ctgttccgca taaaatgccc agtcccacgg ctgcgcgcta aacccgccct
     3661 gctgcttatc gataaccgcc tgtatggagg ctaattcatc gctcgcacgt tgacgcgccg
     3721 ctggaacaat ttcccgcata aagttgagtg ctgcttctgg cgtttttgcc atctgatcgg
     3781 cgattttcca tgcggcataa tgaggaaaac caagcagttt cgcctgctgt gcgcgaatct
     3841 ctaccagacg ttgaatgata gcgcgggtat cattgccatc atttttttcc gctcgcgtcc
     3901 agcccgcaat aaacagtttt tcacgcgtcg cacgatcgcg catttcggca agcgccggtt
     3961 gttgggtggt attcagcagc ggaatcagcc atttgttatc cagacctttc tcgcgagccg
     4021 cctctgccgc cagcgcaatc tcttgctcac tcattcctgc cagctgcgcg atatcgttca
     4081 caaccagacc gccggattta tttgctgcca gtaatcgctg gttaaactgg ctggtcaggg
     4141 tcgcagcttc tgtattcagt acttttaatt ttgctttatc agcttgcgca agtttggctc
     4201 cggcaaggac aaaacgttga tgaatcacct ccaccaggcg gatggattca ctatcaagcc
     4261 ccagggattc acggcgctgc cagacagcat ctacccgcgc gaataattca ccgttcagat
     4321 agatatcatt agccagttcc gccagttcag cggaaaactg ttcatcaaga cgctgtaatt
     4381 catcattggt atgcgccgca gtcatcgcaa aaaagacgct ggtaacgcgg gtaagtaatt
     4441 ctccgctttg ttccagtgcc agaatagtat tgttgaaatc aggcgtttgc gggttaagcg
     4501 cgatggcagc aatttctgcc cgcttttgct gcattccctc atcgaatgcc gggcgatagt
     4561 gatgattggc aatttgatca aaatggggag ccagatacgg tagtgtgctt tgcacaagga
     4621 aaggattcat tgttgtcatt ttcttctcct gaacgcgaga tgttccatag cgtaggctta
     4681 ctgataacgg agtgcaatct tgcaaaccag tattacccgc tcttaagcat cacgtgctat
     4741 gttattgaca cacaaaagcg ttgaggaaca gtgagatgat cgttttagta actggagcaa
     4801 cggcaggttt tggtgaatgc attactcgtc gttttattca acaagggcat aaagttatcg
     4861 ccactggccg tcgccaggag cggttgcagg agttaaaaga cgaactggga gataatctgt
     4921 atatcgccca actggacgtt cgcaaccgcg ccgctattga agagatgctg gcatcgcttc
     4981 ctgccgagtg gtgcaatatt gatatcctgg taaataatgc cggcctggcg ttgggcatgg
     5041 agcctgcgca taaagccagc gttgaagact gggaaacgat gattgatacc aacaacaaag
     5101 gcctggtata tatgacgcgc gccgtcttac cgggtatggt tgaacgtaat catggtcata
     5161 ttattaacat tggctcaacg gcaggtagct ggccgtatgc cggtggtaac gtttacggtg
     5221 cgacgaaagc gtttgttcgt cagtttagcc tgaatctgcg tacggacctg catggtacgg
     5281 cggtgcgcgt caccgacatc gaaccgggtc tggtgggtgg caccgagttt tccaatgtcc
     5341 gctttaaagg cgatgacggt aaagcggaaa aaacctatca aaataccgtt gcattgacgc
     5401 cagaagatgt cagcgaagcc gtctggtggg tgtcaacgct gcctgctcac gtcaatatca
     5461 ataccctgga aatgatgccg gttacccaaa gctatgccgg actgaatgtc caccgtcagt
     5521 aatttttata cccggcgtaa ctgccgggtt attgcttgtc acaaaaaagt ggtagactca
     5581 tgcagttaac tcactcacaa gcaagaacga atgaccgtcg aaacgcaact taatcccaca
     5641 cagcctgtca atcagcaaat ttatcgtatt cttcgtcgcg acattgtcca ttgcctgatt
     5701 gcaccaggca caccgttgtc ggaaaaagaa gtttctgttc gtttcaatgt gtcacgccag
     5761 ccggttcgtg aagcctttat taaactggcg gaaaacggcc tgattcaaat tcgtccgcag
     5821 cgtggcagct acgtcaacaa aatttccatg gcccaggtgc gcaatggcag ttttatccgt
     5881 caggccattg agtgcgcggt ggcgcgtcgg gcggcgagca tgattaccga aagccagtgc
     5941 taccaactgg aacaaaatct tcaccagcaa cgcattgcca ttgagcgcaa gcaactggat
     6001 gatttttttg aacttgatga caacttccat caactcctga cgcagattgc cgactgtcaa
     6061 ctggcgtggg ataccattga gaacctgaaa gcgaccgttg atcgcgtgcg ctatatgagt
     6121 ttcgaccacg tttctccacc agaaatgctg ttacgccagc atcttgatat tttctctgcc
     6181 ctgcaaaaac gtgatggcga tgcggtagaa cgtgcaatga cgcaacattt gcaggaaatc
     6241 agcgaatccg tgcgccagat ccgccaggaa aatagcgact ggtttagcga agagtaattc
     6301 atttcctctc atcccatccg gggtgagagt cttttccccc gccttatggc tcatgcatgt
     6361 atcaaaaaag atgtgagctt gatcaaaaac aaaaaatatt tcactcgaca ggagtattta
     6421 tattgcgccc gttacgtggg cttcgactgt aaatcagaaa ggagaaaata cctatgacga
     6481 cctacgatcg taaccgtaac gcaatcacca ctggcagccg tgttatggtt agcggcaccg
     6541 gtcacactgg caagatcctg tcgattgata ctgaaggtct gaccgctgag caaatccgcc
     6601 gcggaaaaac cgtagttgtt gaaggttgtg aagagaaact ggcacctctg gacctgattc
     6661 gtctcggcat gaactaagcg tgtgaatgcc gccgatggcg gcattgcttt ttttacttca
     6721 cggaatattt cgccacggtc gctttcgcgc catgcgctaa taaagacaag tacgcttccg
     6781 tcacttttgc agtaaacaaa ctattgtctg gcaaatcatc accaaagatc gccttaatcg
     6841 ccagcaatga ctggacgcgc gctttccctt cagcactact ttgtacagcc ttctgaataa
     6901 caggtaacag tgggtcactg atttcaatcg gatttccctg ttcatcaaca ccaccgacat
     6961 aacgcatcca acccgcgacg cccagtgcca gcagatcgaa cttgctgtca tgcgccagat
     7021 gccagcgaac agaatccaac atccgctgtg gcaatttctg gctgccatcc atcgcaatct
     7081 gccaggttcg atgacgtaac gccgggttgc tatagcgttc aattaatcgg ttagcgtaat
     7141 cttgcaaatc aacgccctgc actttcagcg tcggcgcttg ttcctgcaac atcaagccat
     7201 acgccgcata acgataatgt tcatcttcca tgcagtcatt aatgtgctga tatccagcaa
     7261 gataacccaa atacgccagg aatgaatgac tgccgttgag catgcgcaac ttcatctctt
     7321 cataaggcag cacatcacta accagttcgg ctcccgcttt ttcccattct ggacgtccgg
     7381 caacaaagtt atcttctatt acccactggc ggaaaggttc acaggcaacg cccgcaggat
     7441 cgcccacacc ggtaagttgt tcgattttcg ccagcgtatc ctctgtcact gcgggcacaa
     7501 tacggtccac cattgttgat gggaaagtca cgttatcttc gatccattgt gccagtttta
     7561 catcaacagc tttcgcgtat gaagtgacaa cgtcacgcat aacatgaccg ttttctggca
     7621 tgttgtcaca tgacatgacg gtaaatgcgg gaagtcctgc cgctttacgg cgggccagcg
     7681 cctcaacaat cacccctgtt gctgttttcg gctggtgggg attttgcacg tcggcagcga
     7741 ccatcgggtg atcgagcatt aactgtccgg tcgccggaga gtggaaatac cctttttcgg
     7801 tgattgtcag agagacaatc gcgatttgcg gttcacacat cgcagccaac acggtttcta
     7861 agccatccat ctgtacgtgc aaggctttct taacgacgcc aacgacgcga gccgtccacg
     7921 catcggccga catttccgca acggtataaa gattatcttg ctgttgtaaa tcggcaattt
     7981 gctgttcgcc gccgattaag ttgacctcat aatatcccca gtcactgaaa tgttctgtag
     8041 caagaatatc ggcatacacg ccctgatgcg cacggtgaaa tgcaccaaag cctaaatgaa
     8101 taattcttgg ggccaggtta ttacgatcat aaacagggag tgtcgctttt gctgataaca
     8161 aattatttcc cataacaatt ccttaaatat aaatatggca aactatatgt tttgtta
//
LOCUS       DADMKF010000166         8183 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.169, whole genome shotgun sequence.
ACCESSION   DADMKF010000166 DADMKF010000000
VERSION     DADMKF010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8183
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.169"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            <1..2848
                     /gene="yfaL"
                     /locus_tag="JNC24_003478"
     CDS             <1..2848
                     /gene="yfaL"
                     /locus_tag="JNC24_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
                     /protein_id="HAY9160147.1"
                     /translation="ADGKTLVIGNTENDGAVDSIAGTGLITKTGSGDLVLNADNNDFT
                     GEMQIENGEVTLGRSNSLMNVGDTHCQDDPQDCYGLTIGSIDKYQNQAELNVGSTQQT
                     FVHSLTGFQNGTLNIDAGGNVTVNQGSFAGTIEGAGQLTIAQNGSYVLSGAQSMALTG
                     DIVVDDGAVLSLEGDAADLAALQDDPQSIVLNGGVLDLSDFSTWQSGTSYNDGLEVSG
                     SSGTVIGSQDVVDLAGGDNLHIGGDGKDGVYVVVDASDGQVSLANNNSYLGTTQIASG
                     TLMVSDNSQLGDTHYNRQVIFTDKQQESVMEITANVDTRSTTTEHGRDIEMRADGEVA
                     VDAGVDTQWGALMADSSGQHQDEGSTFTKTGAGTLELTASGTTQSAVRVEEGTLKGDV
                     ADIFPYASSLWVGDGAAFVTGADQDIQSIDATSSGTIDISDGTVLRLTGQDTSVALNA
                     SLFNGDGTLVNATDGVTLTGELNTNLETDSLTYLADVTVNGDLTNTSGAVSLQNGVAG
                     DTLTVNGDYTGGGTLLLDSELNGDDSVSDQLVMNGNTAGNTTVVVNSITGIGEPTSTG
                     IKVVDFAADPTQFQNNAQFSLAGSGYVNMGAYDYTLVEDNNDWYLRSQEVTPPSPPNP
                     NPTPDPDPTPDPDPTPDPAPTPAYQPVLNAKVGGYLNNLRAANQAFMMERRDHAGGDG
                     QTLNLRVIGGRYHYTAAGQLAQQEDTSTVQLSGGLFSGRWGTDGEWMLGAVGSYSDNQ
                     GDSRSNMTGTRADNQNHGYAVGLTSSWYQHGNQKQGAWLDNWLQYAWFSNDVSEHEDG
                     TDHYHSSGIIASLEAGYQWLPGRGVVIEPQAQVIYQGVQQDDFTAANHARVSQSQGDD
                     IQTRLGLHSEWRTAVGVTPTLDLNYYHDPHATEIEEDGSTISDDAVKRRGEIKVGVTG
                     NISQRVSLRGSVAWQKGSDDFAQTAGFLSMTVKW"
     gene            complement(2976..3698)
                     /locus_tag="JNC24_003479"
     CDS             complement(2976..3698)
                     /locus_tag="JNC24_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-demethylubiquinone
                     3-O-methyltransferase/2-octaprenyl-6-hydroxy phenol
                     methylase"
                     /protein_id="HAY9160148.1"
                     /translation="MNAEKSPENHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     ITNSFKLGPGVDVNYMLHTQNK"
     gene            3845..6472
                     /gene="gyrA"
                     /locus_tag="JNC24_003480"
     CDS             3845..6472
                     /gene="gyrA"
                     /locus_tag="JNC24_003480"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HAY9160149.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDLAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVEGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            6680..>8183
                     /locus_tag="JNC24_003481"
     CDS             6680..>8183
                     /locus_tag="JNC24_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
                     /protein_id="HAY9160150.1"
                     /translation="MVLLSAGVWALQYAGSGPEKTLSPLVVHNNLQIDLNEPDLFLDS
                     DSLSQLPKDLLTIPFLHDVLSEDFVFYYQNHADRLGIEGSIRRIVYEHDLTLKDKLFS
                     SLLDQPAQAALWHDKQGHLSHYMVLIQRSGLSKLLEPLLFAATSDSQLSKTEISSIKI
                     NSETIPVYQLRYNGNNALMFATYQDKMLVFSSTDMLFKDDQQDTEATAIAGDLLSGKK
                     RWQASFGLEERAAEKTPVRQRIVVSARLLGFGYQRLMPSFAGVRFEMGNDGWHSFVAL
                     NDESASVDTSFDFTPVWNSMPAGASFCVVVPYSHGIAEEMLSHISQENDKLNGALDGA
                     AGLCWYEDSKLQTPLFVGQFDGTAEQAQLPGKLFTQNIGAHESKAPEGVLPISQTQQG
                     EAQIWRREVSSRYGQYPKAQAAQPDQLMSDYFFRVSLAMQNKTLLFSLDDTLVNNALQ
                     TLNKNRPAMVDVIPTDGIVPLYINSQGVAKLLRNETLTSLPKNLEPVFYNA"
BASE COUNT         1979 a         2029 c         2321 g         1854 t
ORIGIN      
        1 tgccgacgga aaaacgctgg ttattggcaa tacagagaat gacggagctg ttgactctat
       61 tgctggtacc gggttgatca ccaaaacagg ttccggcgat ctggtactta atgcagataa
      121 caatgacttt actggtgaga tgcagattga aaacggtgaa gttaccctgg gccgcagcaa
      181 ctccctgatg aatgtcggcg atacgcattg ccaggacgat ccgcaagact gctacggtct
      241 gacgataggg agtattgata agtaccagaa tcaggcagag ctaaacgttg gctccaccca
      301 acaaaccttt gtgcactcat tgacgggctt tcagaatggc actttaaata tcgatgctgg
      361 tggcaatgtt actgttaatc aaggcagttt tgctggcacc atcgaaggtg ctggtcagct
      421 caccattgcg caaaacggca gctacgtgct gtcgggggcg cagtcgatgg cgctaaccgg
      481 cgatatagtc gttgatgatg gtgcggtgct ttcgctggaa ggcgacgcgg cagatctcgc
      541 cgctctccag gacgatccgc agtcgatcgt gttaaacggc ggtgtgctcg atctctctga
      601 tttctccacc tggcagagcg gcacatcata caatgatggc cttgaagtca gtggcagcag
      661 cggaacggtt atcggcagtc aggatgtggt agatcttgca ggtggcgaca atttgcatat
      721 cggcggcgac gggaaagatg gcgtctacgt ggtggtcgat gcgagcgacg ggcaggtaag
      781 tctggcaaac aataatagtt atttgggcac aacacaaatc gcctccggta cgctgatggt
      841 gagcgacaac tcgcagcttg gagataccca ctataaccgc caggttatct ttaccgataa
      901 gcaacaagaa agcgtgatgg agattactgc caatgtcgat actcgctcta cgacgactga
      961 gcatgggcgt gatattgaaa tgcgcgccga cggtgaagtg gcagttgatg cgggggtaga
     1021 cacgcagtgg ggcgcactga tggctgacag cagcgggcag catcaggatg agggtagcac
     1081 atttactaaa acgggggcgg gtacgctgga gctgaccgcc agcggtacaa cgcagtcggc
     1141 agtacgtgtc gaagaaggca ccctgaaagg cgatgttgcg gatatctttc cttatgcttc
     1201 gtcactgtgg gtcggtgatg gggcggcgtt cgttactggc gcggatcagg atattcagtc
     1261 tatcgatgct acttccagcg gcactatcga tatcagcgat ggtacggttt tgcgtctgac
     1321 cgggcaggat acttccgtcg cccttaatgc ctcattgttt aacggcgatg ggacgctggt
     1381 gaatgctacc gatggcgtga cgttgacagg tgagcttaat accaaccttg aaactgacag
     1441 cctgacctat cttgccgacg tgacggttaa tggtgatctg accaatacgt ccggtgcggt
     1501 tagcctgcaa aatggcgtcg ctggcgatac gctgacggta aacggtgatt ataccggcgg
     1561 cggtacgcta ctgctcgata gcgaattaaa cggcgatgac tcggtaagcg atcaattggt
     1621 gatgaacggt aatactgctg gcaataccac ggtggtggtt aactctatta cagggattgg
     1681 tgagccgaca tcgacaggca ttaaagtggt tgatttcgca gctgatccca cgcagtttca
     1741 aaacaatgcg cagttcagtc tggcaggcag cggctacgtc aatatgggag cgtatgacta
     1801 cacgctggtg gaagataaca acgactggta tctgcgatcg caagaagtaa cgccgccatc
     1861 gccacctaat cccaacccga ctcccgatcc tgatcccaca ccggatcctg acccaacccc
     1921 cgacccggcc cctacgcctg cttaccagcc ggtgctgaat gccaaagttg gcggttatct
     1981 caataacctg cgggcggcaa atcaggcgtt tatgatggag cgacgcgatc acgctggtgg
     2041 cgatggtcag acgctgaatt tacgtgttat cggcggacgt tatcattaca cggcggcagg
     2101 gcaactggct cagcaggaag acacttctac ggtgcagctt agcggcggct tgtttagcgg
     2161 gcgctggggc acggatggcg agtggatgct tggggcggtt ggtagctaca gtgataatca
     2221 gggcgacagc cgctcgaata tgaccggaac tcgcgccgat aaccagaacc acggttatgc
     2281 cgttgggctg acctcaagct ggtatcagca cggtaatcag aagcaggggg cctggctgga
     2341 taactggttg cagtacgcgt ggtttagcaa tgatgtttct gaacatgaag atggcacaga
     2401 tcattaccat tcgtcgggga ttatcgcctc gctggaagcc gggtatcagt ggttaccggg
     2461 gcgtggtgtg gtgattgaac cgcaggcgca ggtgatttat cagggcgtgc agcaggatga
     2521 ttttaccgcc gctaaccatg cgcgcgtgtc acaatcgcag ggggatgata ttcagacgcg
     2581 gctgggttta cacagcgaat ggcgtaccgc ggttggtgtc acaccaacat tagatctgaa
     2641 ttattatcac gatccccatg cgacggaaat tgaagaagat ggcagcacta tcagtgacga
     2701 tgcggtgaag cgacggggtg aaataaaagt gggagtcacg ggcaatatca gtcagcgagt
     2761 gtccctgcgc ggcagcgtgg cgtggcagaa agggagtgat gattttgccc agacggcagg
     2821 gtttttgtcg atgacggtga aatggtaatc gctgtctgac catcacaatt gccggatgcg
     2881 gcgtaaacgc cttatctggc ctacggagag catttgtagg cctgataaga cgctgcaagc
     2941 gtcgcatcag gcattggcgc ggccaaacat caacctcact tattctgcgt gtgcagcata
     3001 tagttcacat ccacgccggg gccgagctta aaggaattag tgatcgggtt gtaatgtagc
     3061 ccggttatat gtcgctcttt taaactggtc tgatccaccc agccaagcag ctctgccggt
     3121 ttaataaact tcttcacatc atgcgtacct ttgggcacca tgcgcaaaat atattccgcg
     3181 ccaaccaccg ccatcagcca tgacttgccg ttgcggttaa gcgtcgagaa aaagacatcg
     3241 ccgcctggtt tcaccagttg cgcacaggct ctgaccactg actgcggatc gggtacgtgc
     3301 tccagcatct ccatgcaggt caccacatca tactgcccgg catgttttgc cgcgtgctct
     3361 tccacggttt cctgcacgta atccacctga atgccgcttt ccagcgcgtg cagctttgcc
     3421 acctgcaatg gctcaaagcc catatccaga ccggtcaccg tcgcgccttc gcgcgccata
     3481 ctctcggcca gaatgccgcc gccacaaccg acatcgagca cctttttgcc aaataaaccg
     3541 ccagcacgct cggcaatata gcccagacgc agcgggttaa tgcggtgcaa cggtttgaac
     3601 tcaccttcaa gatcccacca gcgggaggcg acggcttcaa atttagcgat ctcttcgtgg
     3661 tctacgttat ggttttccgg cgatttttcg gcattcattg gcacttctac tccgtaattg
     3721 gcaagacaaa cgagtatatc aggcattgga tgtgaataaa gcgtataggt ttacctcaaa
     3781 ctgcgcggct gtgttataat ttgcgacctt tgaatccggg atacagtaga gggatagcgg
     3841 ttagatgagc gaccttgcga gagaaattac accggtcaac attgaggaag agctgaagag
     3901 ctcctatctg gattatgcga tgtcggtcat tgttggccgt gcgctgccgg atgtccgaga
     3961 tggcctgaag ccggtacacc gtcgcgtact ttacgccatg aacgtactag gcaatgactg
     4021 gaacaaagcc tataaaaaat ctgcccgtgt cgttggtgac gtaatcggta aataccatcc
     4081 ccatggtgac ttggcggttt atgacacgat cgtccgtatg gcgcagccat tctcgctgcg
     4141 ttacatgctg gtagacggtc agggtaactt cggttccatc gacggcgact ctgcggcggc
     4201 aatgcgttat acggaaatcc gtctggcgaa aattgcccat gaactgatgg ccgatctcga
     4261 aaaagagacg gtcgatttcg ttgataacta tgacggcacg gaaaaaattc cggacgtcat
     4321 gccaaccaaa attcctaacc tgctggtgaa cggttcttcc ggtatcgccg taggtatggc
     4381 aaccaacatc ccgccgcaca acctgacgga agtcatcaac ggttgtctgg cgtatatcga
     4441 tgatgaagac atcagcattg aagggctgat ggaacacatc ccggggccgg acttcccgac
     4501 ggcggcaatc attaacggtc gtcgcggtat tgaagaagct taccgtaccg gtcgtggcaa
     4561 ggtgtacatc cgcgcccgtg ctgaagtgga agtcgacgcc aaaaccgggc gtgaaaccat
     4621 tatcgtccac gaaattccgt atcaggtaaa caaagcgcgc ctgatcgaga agattgcgga
     4681 actggtaaaa gaaaaacgcg tggaaggcat cagcgcgctg cgtgacgagt ctgacaaaga
     4741 cggtatgcgc atcgtgattg aagttaaacg cgatgcggtc ggtgaagttg tgctcaacaa
     4801 cctctactcc cagacccagt tgcaggtttc tttcggtatc aacatggtgg cattgcacca
     4861 tggtcagccg aagatcatga acctgaaaga tatcatcgcg gcgtttgttc gtcaccgccg
     4921 tgaagtggtg acccgtcgta ctattttcga actgcgtaaa gctcgcgatc gtgctcatat
     4981 ccttgaagca ctagccgtgg cgctggcgaa catcgacccg atcatcgaac tgatccgtca
     5041 tgcgccgacg cctgcagaag cgaaaactgc gctggtggct aatccgtggc agctgggcaa
     5101 cgttgccgcg atgctggaac gtgctggcga cgatgctgcg cgtccggaat ggctggagcc
     5161 agagttcggc gtgcgtgatg gtctgtacta cctgaccgaa cagcaagctc aggcgattct
     5221 ggatctgcgt ttgcagaaac tgaccggcct tgagcacgaa aaactgctcg acgaatacaa
     5281 agagctgctg gatcagatcg cggaactgtt gcgtattctt ggtagcgccg atcgtctgat
     5341 ggaagtgatc cgtgaagagc tggagctggt tcgtgaacag ttcggtgaca aacgtcgtac
     5401 tgaaatcacc gccaacagcg cagacatcaa cctggaagat ctgatcaccc aggaagatgt
     5461 ggtcgtgacg ctctctcacc agggctatgt taaatatcag ccgctttctg aatacgaagc
     5521 gcagcgtcgt ggcgggaaag gtaaatctgc cgcacgtatt aaagaagaag actttatcga
     5581 ccgactgctg gtggcaaaca cgcacgacca tattctgtgc ttctccagcc gtggtcgcgt
     5641 ctattcgatg aaagtttacc agttgccgga ggccactcgt ggcgcgcgcg gtcgtccgat
     5701 cgtcaacctg ctgccgctgg agcaggacga acgtatcacc gcgatcctgc cagtgaccga
     5761 gtttgaagaa ggcgtgaaag tcttcatggc gaccgctaac ggtactgtga agaaaaccgt
     5821 cctcaccgaa ttcaaccgtc tgcgtaccgc cggtaaagtg gcgatcaaac tggttgaagg
     5881 cgatgagctg atcggcgttg acctgaccag cggcgaagac gaagtaatgc tgttctccgc
     5941 cgaaggtaaa gtggtgcgct ttaaagagtc ttctgtccgt gcgatgggct gcaacaccac
     6001 cggtgttcgc ggtattcgct taggtgaagg cgataaagtc gtctctctga tcgtgcctcg
     6061 tggcgatggc gcaatcctca ccgcaacgca aaacggttac ggtaaacgta ccgcagtggc
     6121 ggaataccca accaagtcgc gtgcgacgaa aggggttatc tccatcaagg ttaccgaacg
     6181 taacggttta gtggttggcg cggtacaggt agatgactgc gaccagatca tgatgatcac
     6241 cgatgccggt acgctggtac gtactcgcgt ttcagaaatc agcatcgtgg gccgtaacac
     6301 ccagggcgtg atcctcatcc gtactgcgga agatgaaaac gtagtgggtc tgcaacgtgt
     6361 tgctgaaccg gttgacgagg aagatctcga taccatcgac ggcagtgccg cggaagggga
     6421 cgatgaaatc gctccggaag tggacgttga cgacgagcca gaagaagaat aattttactt
     6481 cttcatgcca aaagggagct atctcccttg tttgaattga aaagtccagg ctgcaaagtc
     6541 tgggcttttg tcgtattagg gcacggtaaa gtttggctgt gcccgtaaaa aatggctggc
     6601 tatacacaag gaatgtggca atgagtggtg aaaaaaagcg aaaggctggc ggttctatgg
     6661 tcttgtaggt tttggcgcaa tagtactgct ttccgctggt gtctgggcgt tgcaatatgc
     6721 tggcagtggg ccagaaaaaa cgttgtcgcc gctggtggtg cacaacaatc tgcaaatcga
     6781 tctcaatgag ccggacctct ttctcgacag cgactctctg agccagcttc ccaaagatct
     6841 cctcaccatt ccgtttctcc acgatgttct gagtgaagat ttcgttttct attatcagaa
     6901 tcatgccgat cgtctgggca ttgaaggcag cattcgtcgc attgtctatg aacacgatct
     6961 cacgctgaaa gataaactct tttcgtcact cttagatcag cccgcgcagg ctgcgctatg
     7021 gcacgataaa caaggccatc tttcacatta catggtgctg atccagcgca gtggtttaag
     7081 caaactgctg gagccattgt tgtttgccgc taccagcgac agccagttaa gcaaaacgga
     7141 aatcagtagc atcaagataa atagtgaaac tattcctgtt tatcagttgc gctataacgg
     7201 caataacgcc ctgatgttcg cgacttatca ggacaagatg ctggtgtttt ccagcacgga
     7261 tatgttgttt aaagatgatc agcaggatac cgaagccacg gcgatcgcag gtgatttgtt
     7321 gagcggcaaa aaacgctggc aagcaagctt tggcctggaa gagcgtgctg ctgaaaaaac
     7381 gccagtacgc cagcgcatcg tggtcagcgc caggttgctg ggttttggct accagcggtt
     7441 aatgccttct tttgctggcg tacgcttcga aatgggtaac gacggctggc acagttttgt
     7501 ggcgttaaat gatgaatccg ccagcgtaga taccagtttc gattttacgc cggtctggaa
     7561 cagtatgcct gccggggcca gcttctgtgt ggtggtgccg tattcacacg gtattgccga
     7621 agagatgctt tcgcacatca gccaggaaaa cgacaagctg aatggggcgt tagacggtgc
     7681 cgcggggctg tgctggtatg aagactcaaa attgcaaacc ccgctgtttg tcggtcagtt
     7741 tgatggcact gccgaacagg cgcaattgcc agggaaactg tttacgcaaa atattggtgc
     7801 gcacgaaagc aaagcgccag aaggtgtttt gccgataagc cagactcagc agggcgaagc
     7861 gcaaatctgg cgtcgcgaag tgagttcccg atacggtcag tatccgaaag cgcaggcggc
     7921 gcaacccgat caattaatgt cggattattt tttccgcgtg tcgctggcga tgcaaaacaa
     7981 aacgctgctt ttctccctcg atgacacgct ggttaataac gccctgcaaa cactgaataa
     8041 aaaccgcccg gcaatggtgg atgtaatacc cactgatggc atcgttccgc tctatatcaa
     8101 ttcacaaggc gtggcgaaac tgctgcgtaa cgaaacgttg accagtctgc cgaagaatct
     8161 cgaaccggtt ttttataacg ccg
//
LOCUS       DADMKF010000167         8157 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.170, whole genome shotgun sequence.
ACCESSION   DADMKF010000167 DADMKF010000000
VERSION     DADMKF010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8157
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.170"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(<1..449)
                     /locus_tag="JNC24_003482"
     CDS             complement(<1..449)
                     /locus_tag="JNC24_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HAY9160151.1"
                     /translation="MKKQWIVGTALLMLMTGNAWADGEPPTENILKDQFKKQYHGILK
                     LDAITLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFN"
     gene            complement(566..1612)
                     /gene="ansB"
                     /locus_tag="JNC24_003483"
     CDS             complement(566..1612)
                     /gene="ansB"
                     /locus_tag="JNC24_003483"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HAY9160152.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINTDCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            complement(1788..2507)
                     /locus_tag="JNC24_003484"
     CDS             complement(1788..2507)
                     /locus_tag="JNC24_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HAY9160153.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGESSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            complement(2691..3017)
                     /locus_tag="JNC24_003485"
     CDS             complement(2691..3017)
                     /locus_tag="JNC24_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF469 domain-containing protein"
                     /protein_id="HAY9160154.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKTVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEERKLDEVRTSEL
                     FDVWWD"
     gene            complement(3017..3736)
                     /gene="trmB"
                     /locus_tag="JNC24_003486"
     CDS             complement(3017..3736)
                     /gene="trmB"
                     /locus_tag="JNC24_003486"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HAY9160155.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            3897..4949
                     /gene="mutY"
                     /locus_tag="JNC24_003487"
     CDS             3897..4949
                     /gene="mutY"
                     /locus_tag="JNC24_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HAY9160156.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMARFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAAANNSWSL
                     YPGKKTKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRQWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            4977..5252
                     /locus_tag="JNC24_003488"
     CDS             4977..5252
                     /locus_tag="JNC24_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HAY9160157.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            5317..6396
                     /gene="mltC"
                     /locus_tag="JNC24_003489"
     CDS             5317..6396
                     /gene="mltC"
                     /locus_tag="JNC24_003489"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HAY9160158.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLQSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQHTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLDGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            6598..7854
                     /locus_tag="JNC24_003490"
     CDS             6598..7854
                     /locus_tag="JNC24_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HAY9160159.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(7903..>8157)
                     /locus_tag="JNC24_003491"
     CDS             complement(7903..>8157)
                     /locus_tag="JNC24_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HAY9160160.1"
                     /translation="IRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQR
                     YFLALEEGVNLLPGFSPELQGVYSETDADGMKRLYGYVLK"
BASE COUNT         1922 a         2159 c         1987 g         2089 t
ORIGIN      
        1 taattgaaat cttcgctatt cacgatcaga tatttattat tcgttttgat atcgtcgacc
       61 acccgcccac gatcgctggc tgccgcctga aaagagtaaa agttcaccga ccagccagac
      121 gcgggcgttc ctttactggt taacatcgcc gagaatttta ccggtttatc tttcgtccag
      181 gtctgttcga gcagttcgta atctgccaac tgaccgaccc aggtatagag atcgtcactg
      241 gaagagacat cgccttccgc tgaccaggtg gcctgattac ccttagcatc aagatttttt
      301 aaggtgatag catcaagctt gagaatgccg tgatactgct ttttgaattg atcttttaag
      361 atattttcag ttggcggttc gccatctgcc caggcattac cagtcatcaa cataagcagt
      421 gccgtgccaa caatccattg ctttttcacc cgaaaaacct cacgcggaat aatttattcc
      481 ggtgattatc attagggtaa tgaaaattgg caatggacgt aagtctgaag tgaaaaagcc
      541 ccggcacgat accggggcga ggtgattagt actgattgaa gatctgctgg atctgctgcg
      601 gatctttggt ttgcgtcaga gccagctgca gcaggacgcg cgctttttgc gggttcagcg
      661 tgccagaggc gacgaagccg tatttcgcat catccacttc agcatcctga gtggtagcac
      721 ccgtcggtac gcgggaagaa cgcactactg cagtgccgtt tttcgcggcg gttgccaggg
      781 tgtcgaacac ggatttatac aggttaccat tacccacacc agcgctaacg atgccatcat
      841 agcccgcatc taccagtgct ttagccggaa gatcggatgc gttagcgtag ttataaacga
      901 tgccgacttt cggcagctca ttcagcttag agacatcgaa tggcgtatcg ctggtgtgct
      961 tacgtgccgg ggtacgttgg tagtcaatct taccgttgtg aatgtatccc agaggaccgt
     1021 agttaacaga cttgaaggtc gctacgtcgg tggtgttggt tttggtgaca tcgcgaccgt
     1081 ccagtacggt gtcgttcatc accaccagca cgccacgatt agcggaggct ttatcagctg
     1141 cggtcactac cgcgttatac aggttgaatg gaccgtctgc gctcatggac gtggacgggc
     1201 gcattgcgcc gaccatcacc accggtttgt cgcatttcac cgtcaggtcg aggaagtaag
     1261 cggtttcttc catcgtgtcg gtaccgtggg taatgacgaa gccgtcggtt ttatcgcagt
     1321 cggtgttaat ttttttcgcc agtgtcagcc agacattatc gttcatgtcc tgggagccga
     1381 tattcactac ctgctcgcct ttaacgttcg caatgtcttt tagttgcggc accgcattaa
     1441 ccagattttc tacgccaact ttacccgctg tgtagttaga tttggttgcg gagtcaccac
     1501 caccggcaat ggtcccgccg gttgctaaaa tggtgatatt gggtaatgcc aatgctgcac
     1561 cactaaaacc cataaccagt gcggcaagtg ccgtcttttt gaaaaactcc atttcattcc
     1621 tccagttacg tgaacgctac gcattatccc ttagctctgt atgggaaatt tgacgttaaa
     1681 caatttacaa cgtgaatata ttttggagat ctacaaagtt agaggcaggt aacaaaacga
     1741 agaattaaac ggcataaaaa agtattatgc cgtcttaaaa tagaggatta ttttaaattc
     1801 ccgaccaggg ctttgcggct atcttccaga gtcacaacgc ggctacaaac atctttgcca
     1861 aactgctgga aatctttttc ctgctttttc cactctgttt ggattgagga ttgcagcccc
     1921 cccaggcttc ccagcacatt ctgtaatggg ttaccgccgc ttttcagcac cgctttcgcg
     1981 cccatttcat taatgctgtc ctgtaaaatt ccacccattg cctgattcac taattgctgg
     2041 ccttcggcgc gaacctgatc aatggcttta tagtgaaacg tcaggccatc gctgcgcgtt
     2101 tcgataatgc ggttcatctg ctctttcagc tgcgcatcaa gtttggtcag acggctgcgc
     2161 attttgctgc tttcgcccat ctcctgaacg ataattttat ccagcgcaat acgggctttt
     2221 tcgacgcggc ttttcgcgcc ttcatcaatc cacggcagcg tgctgcgtag ttcagcctga
     2281 taatccttcg cctgctcgcg ctgggcggca ttcagggaat attgcttacc gttatacatc
     2341 acgttgccgt ctggcgtgat caccagattg ccgttttcgc ccttcacctg cacggtttgc
     2401 gggctgacaa tcacatcgtc acgcggcgtg acgctgcact ggtagtcggc gtgagcggtc
     2461 attgccgtca ctgaaagtgc tgccgccagc agcattttgc gcatcatagt cttccctcaa
     2521 gaaaaaatca ggccagcatt tgctggcccc agattgataa caaagtacgc gttttccatg
     2581 ccggatgcgg cgtaaacacc ttatcccgcc tactcgatcg tgcatctttc caggcctgat
     2641 aagcgtagcg catcaggcag ttttgcattt gtcatcgccc gtatgctttc ttagtcccac
     2701 caaacgtcga aaagttcgct ggtgcgtacc tcatccagtt tgcgctcttc cagccactta
     2761 cgcacaatcg cctgatgttc ttcggtgcat ttgccgattt cctgcatgca gatcagacct
     2821 tcccaggcca gataaccgct gccgtcaaag gccagtttgt tcggttcgat aacctcgtta
     2881 ataaaatcat caacggtttt atcaatctgt tcttccgatg taccttccgg gaatcgccat
     2941 gccaccgaaa atcctaattc ctggaattcg tcgatgtgca tttttttacg cagacgacgg
     3001 ctacggttct ttgccattat ttcaccctct cgaacattaa gtcccatact ccgtgaccaa
     3061 gacgatgacc acgttgttca aatttcgtca ccggacgtga tgccggacgc ggcacgtaat
     3121 cattgctctc tgacaggttt ttataaccgt caatagaaga catcacttca agcatatgtt
     3181 ccgcataagg ttcccagtcg gtcgccatat ggaatacacc ccccagctgc agtttgcttt
     3241 ttaccagttc ggcaaacggc acctgtacga tacggcgttt attatggcgc gctttgtgcc
     3301 acgggtcagg gaaaaagagc tgcaccatgc gcaatgaatt gtcaggaatc attttatgca
     3361 gcacttcaac cgcatcgtga cacatcacgc gcaggttgct taaaccttct tcatgcgcag
     3421 aagccaggca cgcaccaacg cccggtgaat gcacttcaat gccgaggaag tcctgctcag
     3481 ggcgatcttt agccattgcc accagcgacg cccccatgcc aaaaccaatc tcaagcgtca
     3541 ccggcgcttc acggccaaaa agcgcgggga aatccagcat atcttcgctg aactcaacgc
     3601 ccatcaccgg ccagtagttt tccagcgcat gttcctggcc tttggtcagt cgcccctggc
     3661 ggcgcacaaa actacggata cggcgcagtg ggcggccgtt ttcatcaaat tccggtgaaa
     3721 tgacgtcgtt tttcataaag atttagtcgc ttgtgaaagt gttctgaaaa cgggcattat
     3781 ccaaagttag ttgccggatg caagcatgat aaggccgtgg ctgcggaaag ttccggttta
     3841 caccctgccg tcgctgtgct gcaatcttgc ccccaacaac agtgaattcg gtgaccatgc
     3901 aagcgtcgca attttcagcc caggttctgg actggtacga taaatacggg cgaaaaacgc
     3961 tgccctggca aattgacaag acgccctaca aagtatggct ctcagaagtg atgttgcaac
     4021 aaactcaggt tgcgaccgtt atcccctatt ttgaacgctt tatggcgcgc ttcccgacgg
     4081 tgaccgatct cgccaatgcg ccgctcgacg aagttctcca cttgtggacc gggcttggct
     4141 attacgcccg cgcgcgcaat ctgcataaag cggcacaaca agtggcgacc ttacacggcg
     4201 gtaaattccc ggaaaccttt gaagaagtcg cggcgttacc gggcgtcggg cgttccaccg
     4261 caggcgcgat tctctcgctt tctctgggta agcactttcc gattctcgac ggtaacgtca
     4321 aacgcgtgct ggcgcgctgc tatgctgtaa gcggctggcc tgggaaaaaa gaggtcgaga
     4381 ataaattatg gagtttgagc gagcaggtga cgcccgcggt cggcgtggaa cggtttaatc
     4441 aggcgatgat ggatttgggc gcgatgattt gtacgcgctc gaagccgaaa tgttcgctct
     4501 gtccgctaca aaacggatgt attgccgccg ccaataatag ctggtcgctt tatccgggca
     4561 aaaaaacgaa acagacgctg ccggagcgca ccggctactt tttgctgtta cagcacgaag
     4621 atgaagtatt gctggcgcag cgtccgccga gcggattgtg gggcggttta tactgtttcc
     4681 cgcagtttgc cgacgaagaa agtttgcggc agtggctggc gcaacggcag attgctgccg
     4741 ataacctgac gcaactgacc gcgtttcggc ataccttcag ccatttccac ttagatattg
     4801 tgcctatgtg gcttcccgtg tcgtcattca ccggctgcat ggatgaaggc aatgcgctct
     4861 ggtataactt agcgcaaccg ccgtcagttg gcctggcggc tcccgtggag cgtttgttac
     4921 agcagttacg cactggcgcg ccggtttagc gcgtgagtcg ataaagagga tgatttatga
     4981 gcagaacgat tttttgtact ttcctgcaac gtgaagcaga aggtcaggat tttcagctgt
     5041 accccggcga gctgggaaaa cgcatctata acgagatctc caaagaagcc tgggcgcagt
     5101 ggcagcacaa gcaaaccatg ctgattaatg aaaagaaact caacatgatg aatgccgagc
     5161 accgcaagct gcttgagcag gagatggtca acttcctgtt cgagggtaaa gaggtgcata
     5221 tcgagggcta tacgccggaa gataaaaaat aaaaacagtg ccggagcacg cctccggcaa
     5281 cttgcataaa aacaaacaca acacgcaccc ggaatgatga aaaaatatct cgcgctggct
     5341 ttgattgcgc cgttgctcat ctcctgttcg acgaccaaaa aaggcgatac ctataacgaa
     5401 gcctgggtca aagataccaa cggttttgat attctgatgg ggcaatttgc ccacaatatt
     5461 gagaacatct ggggattcaa agaggtggtg atcgccggtc ctaaggacta cgtgaaatac
     5521 accgatcaat atcagacccg cagccacatc aacttcgatg acggtacgat tactatcgaa
     5581 actatcgccg ggacagaacc tgccgcgcat ttgcgccggg caattatcaa aacgttgctg
     5641 atgggtgacg atccgagttc ggtcgatctc tattccgacg ttgatgatat tacgatttcg
     5701 aaagaacctt tcctttacgg tcaggtggtg gacaacaccg ggcagccgat tcgctgggaa
     5761 ggtcgcgcaa gcaacttcgc ggattatctg ctgaaaaacc gtctgcaaag ccgcagtaac
     5821 gggctgcgta tcatctacag cgtcaccatt aacatggtac cgaaccacct tgataaacgt
     5881 gcgcacaaat atctcggcat ggtccgccag gcgtcacgga aatatggcgt tgatgagtcg
     5941 ctgattctgg caattatgca gaccgaatct tcctttaacc cgtatgcggt cagccgttcc
     6001 gatgcgctgg gattaatgca ggtggtacaa catactgccg ggaaagatgt gttccgctcg
     6061 caggggaaat ccggcacgcc gagccgcagt ttcttgtttg atcctgccag caatattgat
     6121 accggcaccg cgtatctggc gatgctgaac aatgtttatc tcgacggaat tgataaccca
     6181 acatcgcggc gttatgccgt catcaccgcc tataacggcg gcgcaggcag cgtgctgcga
     6241 gtcttttcga atgataagat tcaggctgcc aatattatta acaccatgac gccgggcgat
     6301 gtttatcaga cgctgacgac ccgccatccc tctgcggaat ctcgccgtta tctttataaa
     6361 gtgaataccg cgcaaaaatc ctaccgccgc cgataattcc attaaccgcc cctgacgatg
     6421 ctcaggggca aaaatgttat ccacatcaca atttcgtttt gcaaattggg aatgtttgca
     6481 attatttgcc acaggtaaca aaaaaccagt ccgcgaagtt gatagaatcc catcatctcg
     6541 cacggttaaa tgtgcttttt caaacactca tccgcatcac aatgtgagga aattaacatg
     6601 aatcttaagc tgcagctgaa aatcctctct tttctgcagt tctgtctgtg gggaagttgg
     6661 ctgacgaccc tcggctccta tatgtttgtt accctgaagt ttgacggtgc ttctattggc
     6721 gcagtttata gctcactggg tatcgccgcg gtatttatgc ctgcgctgct ggggattgtg
     6781 gccgacaaat ggttaagtgc gaaatgggta tatgccattt gccacaccat tggcgctatc
     6841 acgctgttca tggcggcaca ggtcacgacg ccggaagcga tgttccttgt gatattgatt
     6901 aactcgtttg cttatatgcc aacgcttggg ttaatcaaca ccatctctta ctatcgcctg
     6961 caaaatgccg ggatggatat cgttactgac ttcccgccaa tccgtatctg gggcaccatc
     7021 ggctttatca tggcaatgtg ggtggtgagc ctgtctggct tcgaattaag ccacatgcag
     7081 ctgtatattg gcgcagcact ttccgccatt ctggttctgt ttaccctgac tctgccgcat
     7141 attccggttg ctaaacagca agcgaatcag agctggacaa ccctgctggg cctcgatgca
     7201 ttcgcgctgt ttaaaaacaa gcgtatggca atcttcttca tcttctcaat gctgctgggc
     7261 gcggaactgc agattaccaa catgttcggt aataccttcc tgcacagctt cgacaaagat
     7321 ccgatgtttg ccagcagctt catcgtgcag catgcgtcaa tcatcatgtc gatttcgcag
     7381 atctctgaaa ccctgttcat tctgaccatc ccgttcttct taagccgcta cggtattaag
     7441 aacgtaatga tgatcagtat tgtggcgtgg atcctgcgtt ttgcgctgtt tgcttacggc
     7501 gacccgactc cgttcggtac tgtactgctg gtactgtcga tgatcgttta cggttgcgca
     7561 ttcgacttct tcaacatctc tggttcggtg tttgtcgaaa aagaagttag cccggcaatt
     7621 cgcgccagtg cacaagggat gttcctgatg atgactaacg gcttcggctg tatcctcggc
     7681 ggcatcgtga gcggtaaagt tgttgagatg tacacccaaa acggcattac cgactggcag
     7741 accgtatggt tgattttcgc tggttattcc gtggttctgg ccttcgcgtt catggcgatg
     7801 ttcaaatata aacacgttcg tgtcccgaca ggcacacaga cggttagcca ctaattacgc
     7861 aaagaaaaac gggtcgccag aaggtgaccc gtttttttat tcttacttca acacataacc
     7921 gtacaatcgt ttcatgccat ccgcatcggt ttcgctatag acaccttgca actccggcga
     7981 aaatcccggc aacaaattca cgccttcttc cagcgcaagg aaataacgtt gaaccgcccc
     8041 accccagact tccccgggaa ccacgcaaag cacgccaggt ggataaggca acgccccttc
     8101 tgccgcaatt cgcccttcgg catcacgaat ccgcaccaac tccacttcac cgcgaat
//
LOCUS       DADMKF010000168         8125 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.171, whole genome shotgun sequence.
ACCESSION   DADMKF010000168 DADMKF010000000
VERSION     DADMKF010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8125
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.171"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            165..319
                     /locus_tag="JNC24_003492"
                     /pseudo
     CDS             165..319
                     /locus_tag="JNC24_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415954.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mRNA interferase"
     gene            365..781
                     /gene="hicB"
                     /locus_tag="JNC24_003493"
     CDS             365..781
                     /gene="hicB"
                     /locus_tag="JNC24_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HicB"
                     /protein_id="HAY9160161.1"
                     /translation="MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALL
                     TAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKVLLLNAFLQSEITQQELARRIGK
                     PKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV"
     gene            860..2266
                     /locus_tag="JNC24_003494"
     CDS             860..2266
                     /locus_tag="JNC24_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAY9160162.1"
                     /translation="MKKYQQLAEQLREQIASGIWQPGDRLPSLRDQVALSGMSFMTVS
                     HAYQLLESQGYIIARPQSGYYVAPQAIKMPKAPVIPVTRDEAVDINTYIFDMLQASRE
                     PSVVPFASAFPDPRLFPLQQLNRSLAQVSKTATAMSVIENLPPGNAELRQAIARRYAL
                     QGITISPDEIVITAGALEALNLSLQAVTEPGDWVIVENPCFYGALQALERLRLKALSV
                     ATDVKEGIDLQALELALQDYPVKACWLMTNSQNPLGFTLTPQKKAQLVALLNQYNVTL
                     IEDDVYSELYFGREKPLPAKAWDRHDGVLHCSSFSKCLVPGFRIGWVAAGKHARKIQR
                     LQLMSTLSTSSPMQLALVDYLSTRRYDAHLRRLRRQLAERKQRAWQALLRYLPAEVKI
                     HHSDSGYFLWLELPEPLDAGELSLAALTHHISIAPGKMFSTGENWSRFFRFNTAWQWG
                     EREEQAVKQLGKLIQERL"
     gene            2511..3656
                     /locus_tag="JNC24_003495"
     CDS             2511..3656
                     /locus_tag="JNC24_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAY9160163.1"
                     /translation="MSKTFARSSLCALTMTIMTAHAAEPPTNLDKPEGRLDIIAWPGY
                     IERGQTDKQYDWVTQFEKETGCAVNVKTAATSDEMVSLMTKGGYDLVTASGDASLRLI
                     MGKRVQPINTALIPNWKTLDPRVVKGDWFNVGGKVYGTPYQWGPNLLMYNTKTFPTPP
                     DSWQVVFVEQNLPDGKSNKGRVQAYDGPIYIADAALFVKATQPQLGISDPYQLTEEQY
                     QAVLKVLRAQHSLIHRYWHDTTVQMSDFKNEGVVASSAWPYQANALKAEGQPVATVFP
                     KEGVTGWADTTMLHSEAKHPVCAYKWMNWSLTPKVQGDVAAWFGSLPVVPEGCKASPL
                     LGEKGCETNGFNYFDKIAFWKTPIAEGGKFVPYSRWTQDYIAIMGGR"
     gene            3674..4687
                     /locus_tag="JNC24_003496"
     CDS             3674..4687
                     /locus_tag="JNC24_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAY9160164.1"
                     /translation="MTYAVEFDNVSRLYGDVRAVDGVSIAIKDGEFFSMLGPSGSGKT
                     TCLRLIAGFEQLSGGAISIFGKPASNLPPWERDVNTVFQDYALFPHMSILDNVAYGLM
                     VKGVNKKQRHAMAQEALEKVALGFVQQRKPSQLSGGQRQRVAIARALVNEPRVLLLDE
                     PLGALDLKLREQMQLELKKLQQSLGITFIFVTHDQGEALSMSDRVAVFNNGRIEQVDS
                     PRDLYMRPRTPFVAGFVGTSNVFDGLMAEKLCGMTGSFALRPEHIRLNTPGELQANGT
                     IQAVQYQGAATRFELKLNGGEKLLVSQANMTGEELPATLTPGQQVMVSWSRDVMVPLV
                     EER"
     gene            4688..5588
                     /locus_tag="JNC24_003497"
                     /pseudo
     CDS             4688..5588
                     /locus_tag="JNC24_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415959.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            5578..6372
                     /locus_tag="JNC24_003498"
     CDS             5578..6372
                     /locus_tag="JNC24_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY9160165.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLIALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGSVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     LLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            6394..7818
                     /gene="patD"
                     /locus_tag="JNC24_003499"
     CDS             6394..7818
                     /gene="patD"
                     /locus_tag="JNC24_003499"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HAY9160166.1"
                     /translation="MQHKLLINGELVSGEGEKQPVYNPATGDVLLEIAEASAEQVDAA
                     VRAADAAFAEWGQTTPKVRAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINILFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHAASSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTPFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
     gene            8028..>8125
                     /locus_tag="JNC24_003500"
     CDS             8028..>8125
                     /locus_tag="JNC24_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic acetyltransferase"
                     /protein_id="HAY9160167.1"
                     /translation="MKVQLYKFTEDKNKTLTFRWTKKHFEFCMDNK"
BASE COUNT         1920 a         1908 c         2222 g         2075 t
ORIGIN      
        1 tattcagttt tgcaaattag cgcaaagaaa ttctggaatc ttccttcctg attttgcatt
       61 gcattctgcc gttgcggcga tttagtgcta ttttcgagca aattacacat aggggtaaac
      121 actaatgttt actttattga tatactcagc ggcagggagg cgatgtgaaa caaagcgagt
      181 tcagacgttg gctcgaatct cagggcgtcg atgtagcgaa tggccgcaac catttgaaac
      241 tcaggtttca tgggaggcgc agtgtcatgc cgcgtcactc ctgcgtaaag caatcctgaa
      301 acaactcggt ttgagttaat cgccaattaa aaaggttaat gacatgcgag agacagtcga
      361 aattatgcgt tatcccgtca ctcttacacc cgcgccggaa ggcggttata tggtttcttt
      421 tgtggatatc cctgaagcgt tgacgcaggg cgaaactgtc gctgaagcga tggaagcggc
      481 aaaagatgct ttactgaccg catttgattt ttattttgaa gataacgagc ttatcccgtt
      541 accttcgcca ttaaatagtc atgatcactt tattgaagta cctttgagcg tcgcctctaa
      601 agtattgctg ttaaatgctt ttttacagtc agaaatcact cagcaagagt tagccaggcg
      661 aattggcaaa cctaaacagg agattactcg cctatttaac ttgcaccatg cgacaaaaat
      721 cgacgccgtc cagcttgcgg caaaggcgct tggcaaagag ttatcgctgg tgatggttta
      781 attacagtta acgaaaagtt gtcattttta acaactgata tagactgccg aatcatctgc
      841 acataattac gattcgataa tgaaaaaata ccagcagctt gcagaacaat tacgcgagca
      901 gattgcgtcg ggtatctggc aacccggcga tcgtttgcct tcgttgcgtg accaggtggc
      961 gctttcaggc atgagcttta tgactgtcag ccatgcctat cagttgctcg aaagtcaggg
     1021 atatattatc gcacgaccgc agtcgggtta ttacgttgcg ccacaggcaa taaaaatgcc
     1081 gaaagcgcca gtcattccag tcactcgaga tgaagcagtc gatatcaaca cttatatttt
     1141 tgatatgttg caggccagtc gcgaaccgtc ggtcgttccg tttgcctcgg cctttcccga
     1201 cccgcgactt ttccccctcc aacaactaaa ccgctcgctg gcgcaggtaa gcaaaaccgc
     1261 cacagcgatg agcgtgattg aaaacttacc gccaggaaac gcagaactgc gtcaggctat
     1321 tgctcgtcgc tatgccttac agggcatcac catttctcct gatgaaattg tcattactgc
     1381 cggggcgtta gaggcattaa acctcagttt gcaagcggta actgaaccgg gcgattgggt
     1441 gatagtagag aatccttgtt tctacggtgc gttgcaggcg ctggagcggc tacggctgaa
     1501 ggcgttatcg gtggcgacgg atgttaaaga agggatcgat cttcaggcgc tggaactggc
     1561 gttgcaggat tatccggtga aagcgtgctg gctgatgact aatagccaga acccactcgg
     1621 atttacctta acgccgcaaa aaaaagcaca actggtggcg ttactcaatc agtacaacgt
     1681 aacgctgatt gaagatgacg tttacagcga actttatttt ggacgggaaa aaccgctgcc
     1741 tgcgaaagcg tgggatcgcc acgatggcgt tttgcattgc tcttcgtttt cgaaatgtct
     1801 ggtgcctggt tttcgtattg gttgggtcgc cgccggaaaa catgcacgta aaattcaacg
     1861 cttgcagttg atgagtacgc tttccaccag ctcaccgatg caacttgcgc tggtggatta
     1921 cctttccacg cgccgatacg acgcccatct tcgtcgcctg cgtcgccagc ttgcggaacg
     1981 taaacaacgt gcctggcagg cactgctgcg ttatctgcct gcggaagtta aaattcatca
     2041 tagtgacagc ggttattttc tctggctgga gctccccgag ccgttagatg ccggcgaatt
     2101 aagcctggcg gcactgacgc atcatatcag tattgcgccg ggtaaaatgt tttctaccgg
     2161 tgaaaactgg tcacgttttt ttcgttttaa taccgcgtgg cagtggggag aacgtgaaga
     2221 acaggcggta aaacaattag gcaaacttat tcaagaacgg ctgtaatagc gtttaattta
     2281 attcctctta gattgggtaa tatgaatttc gaatagcagt catatttcct aaccccttgt
     2341 ctatactcca gaagataacc ttacagacgg cataatgcgc ggtagctcac aacctgatta
     2401 aattttctca gggacgaagg tgtgcctgca agccgccgtc tatggttaaa caaggagata
     2461 tttttacggc acggcggctg aacaattaat tacgacagga gtaagacctt atgagcaaga
     2521 catttgcccg cagcagcctg tgtgcgctca ccatgacaat aatgaccgct cacgccgccg
     2581 aaccacctac caatttagat aaaccggaag ggcgactgga tattatcgcc tggccgggat
     2641 acatcgaacg cggacaaact gataaacaat acgactgggt aacgcaattt gaaaaagaga
     2701 caggctgcgc ggtgaatgtg aaaaccgccg cgacttccga tgaaatggtc agtctgatga
     2761 ccaaaggggg ttacgatctg gttacggcat ccggcgatgc ctcgctgcgt ttgattatgg
     2821 gcaaacgcgt gcagccgatt aataccgcct tgattcccaa ctggaaaacg ctcgatccgc
     2881 gcgtggttaa aggcgactgg tttaatgttg gcggcaaagt ttacggcaca ccttaccaat
     2941 gggggccgaa cctgctgatg tacaacacta aaaccttccc gacgccgccg gatagctggc
     3001 aagtggtttt tgttgagcaa aatctgccgg acggcaagag caataaaggc cgcgttcagg
     3061 cttatgatgg ccctatctac attgcggacg ctgcgttgtt cgttaaagcc actcagccgc
     3121 agttgggcat cagcgatccg tatcaactaa ccgaagaaca gtaccaggcg gtgctgaaag
     3181 tgctgcgcgc tcaacacagt ttgatccatc gctactggca tgacactacc gtgcaaatga
     3241 gcgatttcaa aaacgagggt gtggttgctt ccagtgcctg gccctatcag gccaacgccc
     3301 tgaaagccga aggccagcct gttgctaccg ttttcccgaa ggagggtgtt accggttggg
     3361 ctgataccac catgctgcat agcgaagcga aacatccggt ttgcgcctac aaatggatga
     3421 actggtcatt aacgccaaaa gtgcagggcg atgtggcggc ctggtttggc tcgttaccgg
     3481 tagtgccgga agggtgtaaa gccagtccgt tattaggcga aaaaggttgt gaaaccaacg
     3541 gttttaacta tttcgacaaa atcgccttct ggaaaacgcc tatagcagaa gggggcaagt
     3601 ttgttcccta cagtcgctgg acgcaggatt acattgccat tatgggcggt cgctaacttc
     3661 gctggggtgc tttatgacgt acgcagtgga gtttgacaac gtctcgcggt tgtacggtga
     3721 cgtgcgcgca gtagatggcg tcagtattgc gataaaagat ggtgagttct tctctatgct
     3781 ggggccgtcc ggctccggca aaaccacctg cctgcgcctg attgctggct tcgaacagct
     3841 ttccggcggg gctatctcta tctttggtaa acccgccagc aatctgccac cgtgggagcg
     3901 ggacgtgaat actgtctttc aggactacgc gctatttccg catatgtcga ttcttgacaa
     3961 tgtcgcctat gggctgatgg tcaaaggcgt gaataaaaag cagcggcacg caatggcgca
     4021 agaggcgctg gagaaagtgg cgttggggtt tgtacagcaa cgtaaaccgt cacaactttc
     4081 tggtggtcag cgccagcggg ttgctatcgc cagagcattg gtgaatgaac cgcgcgtatt
     4141 gctgttggat gaaccgctcg gcgcactgga tctcaaattg cgtgagcaga tgcagctgga
     4201 actgaaaaaa ctgcaacagt ctctcggtat cacttttatc ttcgttaccc acgatcaggg
     4261 cgaagcgtta tcgatgtccg atcgtgtggc ggttttcaat aatggacgca ttgagcaggt
     4321 cgattccccg cgcgatctct atatgcgccc gcgcacgccg tttgttgccg ggttcgttgg
     4381 tacatcgaat gtttttgatg gactgatggc agagaaactt tgtggcatga cgggaagctt
     4441 cgccctgcga ccggaacata tccgcctcaa cacccctggt gaactgcagg ccaatggcac
     4501 gatccaggcg gtgcaatatc agggcgcggc aactcgtttt gaactgaaat tgaacggcgg
     4561 tgaaaaactg cttgtgagtc aggccaatat gacaggcgaa gaactgcctg ccacgctcac
     4621 gcccggacaa caggtgatgg tttcctggtc gcgtgatgtg atggtgccgc tggttgagga
     4681 gaggtgaatg gcgatgaatg tattgcaatc accttcacgt ccaggtctgg ggctgttttt
     4741 actgctgctt ggcccgctaa tgtggtttgg cattgtctat ttcggctcgc tgctgacact
     4801 gttatggcag ggattttata cttttgacga tttcaccatg tcggtaacgc cggaactgac
     4861 gctggcgaat atccgtgcgc tgtttaatcc ggcgaattac gacatcattc tccgcacgct
     4921 gactatggct gtggcagtca ctatcgccag cgccattctg gcttttccaa tggcgtggta
     4981 tatggcgcgc tataccagcg ggaaaatgaa agcgttttta tattgcggta atgttgccga
     5041 tgtgggcgag ctacattgtt aaagcctatg cctggacgtt attgctggca aaagatggcg
     5101 tggctcagtg gtttttacaa catcttgggc tggaaccact gctgactgcg ttccttacat
     5161 tacctgcggt gggcggaaat acgctgtcaa cttccgggct ggggcgcttt ctggtgtttc
     5221 tctatatctg gttgccgttc atgatcctgc ccgttcaggc ggcgcttgag cgtttgccgc
     5281 cgtcattgtt gcaggcgtcg gctgatctcg gcgcacgtcc acgacaaacc tttcgctatg
     5341 tggtgctgcc gctggcaatc ccgggtattg ccgctggctc tatctttacc ttctcactca
     5401 ctctgggcga ttttatcgtc ccgcagctgg ttggtcctcc aggatatttt atcggcaata
     5461 tggtttattc ccagcagggg gcgattggca atatgccgat ggcggcggca ttcaccttgg
     5521 tgccgattat tctcatcgca ctgtacctgg cgttcgtgaa acgtctggga gcgttcgatg
     5581 cactctgaac gcgcaccgtt tttcctcaaa ctggcggcct ggggcggcgt tgttttccta
     5641 cattttccca tcctgataat cgccgcctat gcgtttaaca ctgaagatgc ggcgtttagt
     5701 tttccaccgc agggcctgac gctgcgctgg tttagcgtgg cagcacagcg tagtgatatt
     5761 cttgatgccg tgacactgtc acttaaagtg gcggcgctgg cgacattaat tgcgctggtg
     5821 ttagggacgc tggcagctgc cgcgctgtgg cgacgagact ttttcggcaa aaacgccatt
     5881 tcgctgttac tgctgctgcc cattgcgctg ccgggcagcg tcactggtct ggcgttatta
     5941 accgccttta aaaccatcaa tctggagccg ggatttttca ccatcgtggt cggtcatgcg
     6001 actttttgtg tagttgtggt gtttaacaat gtcatcgccc gttttcgccg cacctcctgg
     6061 agtctggttg aggcgtcaat ggatcttggg gccaatggct ggcaaacctt ccgctacgta
     6121 gtgttgccga atctcagttc ggcgttactg gcaggaggaa tgctggcgtt tgccttgtcg
     6181 ttcgatgaaa tcatcgttac gacctttacg gcaggtcatg aacgaacgtt actgttgtgg
     6241 ttgctcaatc agcttgggcg accgcgtgat gtaccggtaa ctaacgtggt ggcactgctg
     6301 gttatgttgg taacaacctt gccgatcctg ggggcctggt ggctaacccg cgaaggcgac
     6361 aatggtcaat aaccactgat acaggaatat gctatgcaac ataagttact gattaacgga
     6421 gaactggtta gcggcgaagg ggaaaaacag cctgtctata atccggcaac gggggacgtt
     6481 ttactggaaa ttgccgaggc atccgcagag caggtcgatg ctgctgtgcg cgcggcagat
     6541 gcagcatttg ccgaatgggg gcaaaccacg ccgaaagtgc gtgcggaatg tctgctgaaa
     6601 ctggctgatg ttatcgaaga aaatggtcag gtttttgccg aactggagtc ccgtaattgt
     6661 ggcaaaccgc tgcatagtgc gttcaatgat gaaatcccgg cgattgtcga tgtttttcgc
     6721 tttttcgcgg gtgcggcgcg ctgtctgaat ggtctggcgg caggtgaata tcttgaaggt
     6781 catacttcga tgatccgtcg cgatccgttg ggggtcgtgg cttctatcgc accgtggaat
     6841 tatccgctga tgatggccgc gtggaaactt gctccggcgc tggcggcagg gaactgcgta
     6901 gtgcttaaac catcagaaat taccccgctg accgcgttga agttggcaga gctggcgaaa
     6961 gatatcttcc cggcaggcgt gattaacata ctgtttggca gaggcaaaac ggtgggtgat
     7021 ccgctgaccg gtcatcccaa agtgcggatg gtgtcgctga cgggctctat cgccaccggc
     7081 gagcacatca tcagccatgc cgcgtcgtcc attaagcgta ctcatatgga acttggtggc
     7141 aaagcgccag tgattgtttt tgatgatgcg gatattgaag cagtggtcga aggtgtacgt
     7201 acatttggct attacaatgc tggacaggat tgtactgcgg cttgtcggat ctacgcgcaa
     7261 aaaggcattt acgatacgct ggtggaaaaa ctgggtgctg cggtggcaac gttaaaatct
     7321 ggtgcgccgg atgacgagtc tacggagctt ggacctttaa gctcgctggc gcatctcgaa
     7381 cgcgtcagca aggcagtaga agaggcgaaa gcgacagggc acatcaaagt gatcactggc
     7441 ggtgaaaagc gcaagggtaa tggctattac tatgcgccga cgctgctggc tggcgcatta
     7501 caggacgatg ccatcgtgca aaaagaggta tttggtccag tagtgagtgt tacgcccttc
     7561 gacaacgaag aacaggtggt gaactgggcg aatgacagcc agtacggact tgcatcttcg
     7621 gtatggacga aagatgtggg cagggcgcat cgcgtcagcg cacggctgca atatggttgt
     7681 acctgggtca atacccattt catgctggta agtgaaatgc cgcacggtgg gcagaaactt
     7741 tctggttacg gcaaggatat gtcactttat gggctggagg attacaccgt cgtccgccac
     7801 gtcatggtta aacattaagt tagatgttag tggctgttat aggacggcca ctatcgtttt
     7861 aggttctgag tttaatggcg gttaagtatt agaatgatta cattaataac tagcgtaaaa
     7921 attaggctaa atgtttgatg gcgatttttc atcctgtcac agttgattag cataaggctt
     7981 tagagtatcg ttattaaact aataatttat aagtagaagt attaacaatg aaagttcaat
     8041 tgtacaaatt cacggaagat aaaaataaaa cccttacctt cagatggacg aaaaaacact
     8101 ttgagttttg tatggataat aaaat
//
LOCUS       DADMKF010000169         8104 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.172, whole genome shotgun sequence.
ACCESSION   DADMKF010000169 DADMKF010000000
VERSION     DADMKF010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8104
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.172"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(101..796)
                     /locus_tag="JNC24_003501"
     CDS             complement(101..796)
                     /locus_tag="JNC24_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HAY9160168.1"
                     /translation="MDNRSEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVVLFSAVERGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(789..2216)
                     /locus_tag="JNC24_003502"
     CDS             complement(789..2216)
                     /locus_tag="JNC24_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding protein"
                     /protein_id="HAY9160169.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVANAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVWFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(2227..2946)
                     /locus_tag="JNC24_003503"
     CDS             complement(2227..2946)
                     /locus_tag="JNC24_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HAY9160170.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAVKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVVHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
     gene            complement(3474..4328)
                     /locus_tag="JNC24_003504"
     CDS             complement(3474..4328)
                     /locus_tag="JNC24_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAY9160171.1"
                     /translation="MDALSRLLMLNAPQGTIDKNCVLGSDWQLQHGAGELSVIRWHAL
                     TQGAAKLEMPTGEIFTLRPGNVVLLPQNSAHRLSHVDNESTCIVCGTLRLQHSARYFL
                     TSLPETLFLAPVNHSVEYNWLREAIPFLQQESRSAMPGVDALCSQICATFFTLAVREW
                     IAQVNTEKNILSLLLHPRLGAVIQQMLEMPGHAWTVESLASIAHMSRASFAQLFRDVS
                     GTTPLAVLTKLRLQIAAQMFSREMLPVVVIAESVGYASESSFHKAFVREFGCTPGEYR
                     ERVRQLAP"
     gene            4554..5879
                     /locus_tag="JNC24_003505"
     CDS             4554..5879
                     /locus_tag="JNC24_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridine nucleotide-disulfide oxidoreductase"
                     /protein_id="HAY9160172.1"
                     /translation="MNKYQAVIIGFGKAGKTLAVTLAKAGWRVALIEQSNAMYGGTCI
                     NIGCIPTKTLVHDAQQHTDFVRAIQRKNEVVNFLRNKNFHNLADMPNIDVIDGQAEFI
                     NNHSLRVHRPEGNLEIHGEKIFINTGAQTVVPPIPGITTTPGVYDSTGLLNLKELPGH
                     LGILGGGYIGVEFASMFANFGSKVTILEAASLFLPREDRDIADNIATILRDQGVDIIL
                     NAHVERISHHENQVQVHSEHAQLAVDALLIASGRQPATASLHPENAGIAVNERGAIVV
                     DKQLHTTADNIWAMGDVTGGLQFTYISLDDYRIVRDELLGEGRRSTDDRKNVPYSVFM
                     TPPLSRVGMTEEQARESGADIQVVTLPVAAIPRARVMNDTRGVLKAIVDNKTQRILGA
                     SLLCVDSHEMINIVKMVMDAGLPYSILRDQIFTHPSMSESLNDLFSLVK"
     gene            5988..6224
                     /locus_tag="JNC24_003506"
     CDS             5988..6224
                     /locus_tag="JNC24_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HAY9160173.1"
                     /translation="MFKKSVLFATLLSGVMEFSTNADDKIILKHISVSSVSASPTVLE
                     DTIADIARKYNASSWKVTSMRIDNNSTATAVLYK"
     gene            6236..6829
                     /gene="rclC"
                     /locus_tag="JNC24_003507"
     CDS             6236..6829
                     /gene="rclC"
                     /locus_tag="JNC24_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine species resistance protein
                     RclC"
                     /protein_id="HAY9160174.1"
                     /translation="MEKYLHLLSRGDKIGLSLIRLSIAIVFMWIGLLKFVPYEADSIT
                     PFVANSPLMSFFYEHPEDYKQYLTHEGEYKPEARAWQSANNTYGFSNGLGVVEVIIAL
                     LVLANPVNRWLGLLGGLMAYTTPLVTLSFLITTPEAWVPALGDAHHGFPYLSGAGRLV
                     LKDTLMLAGAVMIMADSAREILKQRSNESSSTLKTEY"
     gene            complement(6989..7858)
                     /locus_tag="JNC24_003508"
     CDS             complement(6989..7858)
                     /locus_tag="JNC24_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308365.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAY9160175.1"
                     /translation="MEFSVLSNNLKMPMMGFGVFQVTDKNVCKQSVLNAIRTGYRLID
                     TAAVYGNEDAVGEAVREAISEGLCTRDELFITSKLWGQDMLNQDTAAAGIEASLKKSG
                     LEYFDLYLLHQAMRDYFSVWRALEDAYEEGKLKAIGVSNFYPHVLANFCETVRVKPMV
                     NQVELHPYFAQPEALATMKYYNVQPEAWAPLGGGRHKPFENNLLQSIADAHQKSISQV
                     ILRWNIQRGVVVIPKSTHQQRIEENFAIWDFSLTEKEMAQISSLDLGYVGESVKHFNP
                     EFVRGCLAVKIHD"
BASE COUNT         2193 a         1982 c         1732 g         2197 t
ORIGIN      
        1 agcataacca tgacggaacg gggttaagtc aggttgtcct taacgctatt ttcaacctcg
       61 tatgccttct tcaggtttat gttcagatct catatctctc tcagcaatcc tcaataatca
      121 gatacaccgc tttcaccgga ccatgaaccc cgacgacttt gataagctca atatccgccg
      181 ttgagctggg accactgatg atgttaatac atgacggcat tcgttcaccg gcctgcgctt
      241 tctgatgcaa tttttctgcg agttgcgcta cacgcggcag gattgtgctt ttacgcagga
      301 taaaaagaga agattccggg agcaggctca atgaacgccc gcgctcgacg gcggaaaaaa
      361 gaaccacgcc tcccgattcg gttaaaccat attcagcata cacgacaccc actttagcct
      421 gctctgcctg cgagatattc tcggcacctt tcgccggatc ccaaacaacg gcattgcatt
      481 cctgctgcaa acgttcacta atccccaatt cctccagcct cgtgtcaccg ctaatcacga
      541 ccgactgatc tcccagctct ttacacagac gtattgcagc ttctgccgcc ttcgcctcgc
      601 tggtcagctc acagcgcgtc aacataacat cgctggcaaa ctgaataaac gcgtcacagc
      661 gctgctgttg gttaagttgg gtaagccgct cgttagcata gttgttaagc ggcgcatctt
      721 ctgcttgcgg ttcaagtcgc agcgggcgac ccagtgcctg agcaacgtta ttcaaaaatt
      781 cgctccgatt atccattctt tttctcctgc gcctgatgtt tcttaaacca actacggaaa
      841 ctctctccgt cagcttcagg aagatcgcgt gcttccatcc agtcgctaat cgcgccaaat
      901 ttgagtggtg ttttgccgcc attgataaac cagcttgccg catgagcgcc ggccatcatc
      961 ccgactttcc acaatcctgg atgactattg gcataagcga acatttttat cgcccgttgc
     1021 tctgcttttg cggtgatccc tttttcagcc atcacccgac gatgacgcaa aatcagtttt
     1081 gacagcggaa tacgcaccgg acacacgctg tcacaagcag tgcataaaga gcaggcgtag
     1141 ggtaaatctt taaaatcttt atagccgcca agtagcggag aaatcaccgc accaattggc
     1201 cctggataaa tagagccata tccatgaccg ccaatatggc gatatgccgg acaagtattc
     1261 atacaagccc cgcagcgaat acagcgcagc acatcccgaa attcagaggc cagcacctca
     1321 gaacgcccgt tatcgacaat aaccagatga aactcttcag gaccatcaac gtgccccgct
     1381 tcgcgcggtc ctgtcagcca ggtgttgtat cccgtcaaac gtgcaccaac ggcactgcgc
     1441 gccagcatgg tgatcaatac atctacctcg gcaaacgtgg gggcaatacg ctccattccc
     1501 atcacagcaa tatgcgtttt aggcagcgtg gtacacattc gcgcattacc ttcattggtc
     1561 accaggcata ccgaacctgt ctctgccacc gcgaaattac agccggtaat acctatttca
     1621 gcactgagga aatcttcgcg gattttttgc cggatgaata aggtcatcgc ttcaggcgtt
     1681 tccggcccct catagcccag acgttcgtgt agcactcgac ggatctgatg gcgatcttta
     1741 tgaattgccg ggaccacaac atgagatggc ggatcttgat ccagctggag aatatattca
     1801 cccagatcgg tttcaatcac ctgaatgcca gcatcctgca acacatgatt gacaccaatc
     1861 tcttcggtca ccatcgattt agatttcacc accttccggg catttttgcg ttgggcaacc
     1921 tgtaaaatgt agcgggtagc gtcttctttg gtttttgcaa accagacgtg accgccgttt
     1981 tgcgtcactt tttctgagag ctggtacaga taagcgtcga gattactcag aacatgatca
     2041 cgtatctggg cggcccgatc gcgccactcc tcccagtgcc ccaattcatc gaccattttt
     2101 tgccgatttg ccccaatacg ctgctgcgcg tttgccaccg ctttgcgcat gatcggatct
     2161 tcaatttgct gacggatgcg tgtcttaaaa tctgtattac tggttttgat cgacatcttt
     2221 atatcctcag cggctcatca acacttcagc aatatgcatc actttgactt tctgcccttc
     2281 ccgttgtaat cgcccactga tgttcagcag gcaactcacg tcagcaccaa ttaaatactc
     2341 agggcggact tccatcaggt gcacaacctt ttctttcacc atctcgccgg atatttcggc
     2401 cattttgacc gagaacgtgc cgccaaatcc gcagcaggta tcctgttcag caaaggtcaa
     2461 cagctccagt ccacgcacat ttttcagcag cgtaagtggc tcgtccttca ctcccagctt
     2521 acgggccagg ctacaagatg ggtgatacac agctctccct tgcaaactgg cacctacatc
     2581 gactacccct aatttattaa caataaaaga ggtgagatcc tgcatacgcg cggcaacctt
     2641 ttcggcacgt gatgcccatt caggttcatc cgccagatac gtcgggtaac tttttacggc
     2701 ataggtgcaa gagccagccg gtgaaataat gggatcgtcg ttatcctcca gtgcggcgat
     2761 cagatttttc atccctggaa tcgcttcttt gatataaccg ctattgatcg caggctgacc
     2821 gcagcatccc tgtttctccg ggaaatttac gcgacagccg agtttttcca gtagcagcac
     2881 ggagtctcgt gccattcttg atttcagggc gtcaccaata caggtgacaa agaaattgac
     2941 attcacaact aatactccat tacttcatgc ccatttatgc ccgatggttc ccctccttcc
     3001 gggggatcaa tcatcaataa atatggcgtt cttttttaat tggctagcaa cataccaccc
     3061 ctcatttgtg tgacaaatga taatctttca tccaaacttc aaatgcaata actaatttta
     3121 tttctttcgg catcgctcac attttatggg gaaatcgacg ttcatttttg gcataagaaa
     3181 tccatttaaa aaggctacat cccttatacc tattcagagc tacttattca ggggttattg
     3241 agatatacac tgatggtgta caaaaaaatc accgacaata atcacgtatc aagtaatagt
     3301 cggggtttta tttaattgaa gcaatttagt ttgcattttt atttaaaaac tgagctagag
     3361 tccatcagaa aaaaatatat aaagcccaaa ccaccgttgg aaatagaaat agttgaaagg
     3421 ctcacaactc ccggaatgcg gattatcttt gccctgattt ctggcggttt tattcagggt
     3481 gcaagctgtc tgaccctttc ccgatattct cccggagtac aaccaaactc gcggacaaac
     3541 gccttgtgaa aagatgattc actggcatag cctactgact cagcgatcac cacaacaggg
     3601 agcatttccc gggaaaacat ctgggccgct atttgtagac gcaactttgt taatacagcc
     3661 agcggcgtgg ttccggaaac atcacggaaa agctgggcaa aacttgcccg agacatgtgg
     3721 gcgatgctgg ccagcgattc gacggtccag gcgtgtcctg gcatttccag catttgctgt
     3781 attaccgcac caagacgtgg atgcagaagc aaactgagaa tgtttttctc tgtattaacc
     3841 tgtgcaatcc actcacgcac cgcgagggta aagaatgtag cgcagatctg gctacacagt
     3901 gcatccactc ccggcatcgc cgatctggat tcctgttgta aaaacgggat cgcctccctc
     3961 agccagttat attccacgct gtggttaacc ggtgctaaaa acagcgtttc cggcaaagac
     4021 gttaaaaaat aacgcgccga atgttgcaac cgaagagtgc cgcagacaat acaggtcgat
     4081 tcattatcga catgactcag gcgatgagct gaattttgtg gtagcaggac cacatttccc
     4141 gggcgtaatg taaaaatctc tcccgtcggc atttccagct tcgccgctcc ttgcgttaac
     4201 gcatgccaac gaataaccga taattccccg gcaccatgtt gaagctgcca gtcacttcct
     4261 aacacgcaat tcttatcgat cgttccttgt ggagcgttaa gcatcaacaa acggctaagg
     4321 gcatccatat cagactcctg agtggttttt gattacaaac accaacaatc ttagtaaata
     4381 actaatctaa taccatcaaa tggttaatca tgatttttgt tttgtgattc tgattaagaa
     4441 aaagaaaata ccagacgata agacaaaata tcaagcgttg tgaagaaatg gtatttgctc
     4501 ttttgcgtct atagtcatga tgtcaaatga acgtgtttcg acaggaaatc atcatgaata
     4561 aatatcaggc agttattatt ggttttggca aggctggaaa aacattagcc gtcacgctgg
     4621 caaaagcagg ttggcgagtg gctctcatcg aacaatcaaa tgcaatgtat ggcgggacct
     4681 gtattaatat cggctgcatc ccaaccaaaa cattggttca tgacgcacag cagcacacag
     4741 attttgtccg tgccatacag cgtaaaaatg aagtggttaa ttttttacgt aataagaatt
     4801 ttcataatct tgcggatatg cccaatatcg acgtgatcga cggccaggcg gagtttatca
     4861 ataatcatag cctgcgtgtt catcggcctg agggaaatct ggaaattcat ggcgagaaaa
     4921 tttttattaa taccggtgca caaaccgtgg ttccgccaat tcctggaatt accaccacgc
     4981 ctggtgtata tgacagcacc ggattactta atctaaaaga attgcctggg catttaggta
     5041 ttttgggcgg cggatatatt ggcgttgagt tcgcctctat gttcgctaat tttggcagca
     5101 aggtaaccat tttagaagca gcttcgctgt ttttgcctcg ggaagatcgg gatattgctg
     5161 ataatatcgc gacgatttta cgcgatcagg gcgtcgatat tatcctcaat gcccatgtgg
     5221 agcgaatcag tcaccatgaa aatcaagtgc aagtgcatag cgagcacgcc caactggcgg
     5281 tggatgcact gttaatagct tccggtcgtc aaccggctac cgcttcgtta catccagaaa
     5341 atgccggtat cgcagtaaac gagcgcgggg caattgtcgt tgacaagcaa ttacatacca
     5401 ccgcagacaa tatttgggcg atgggagatg ttaccggcgg gctgcaattt acttatattt
     5461 cactggatga ttaccgcatt gtgcgtgatg agttgctggg agaaggcaga cgtagtactg
     5521 atgatcggaa aaatgtgcct tattccgtat tcatgacacc gccactgtcc agggttggta
     5581 tgacagaaga acaggccaga gagagtggtg ctgatattca ggtggtgaca ttgcctgtag
     5641 ctgcaattcc gcgtgccaga gtgatgaatg atactcgtgg ggtattaaaa gcgattgttg
     5701 ataataaaac ccaacgtata ttaggggcat cactgctgtg tgttgactcc cacgagatga
     5761 tcaatatagt gaaaatggtg atggatgccg ggctgcctta tagcatatta cgcgatcaga
     5821 tatttactca tccgtcgatg agcgaatcac tcaatgatct attttcatta gtcaaataaa
     5881 ctcaaaatca gacgccagaa caaatattct ggcgtctcag agaaaagaat cttattaatt
     5941 ctctgttact ctataacttc ttaatcactt cattgatggt attttatatg tttaaaaaat
     6001 ctgttttatt tgcaacacta ttatctggcg ttatggaatt ttccaccaat gcagatgata
     6061 aaataattct gaaacatatc agcgtctcgt cagtatcagc atcaccgaca gttctggagg
     6121 ataccattgc tgatatagcc agaaaatata atgcttcatc ctggaaagtc acatcgatgc
     6181 gaattgataa taattcaacc gcaacagcag tattgtataa ataaggatgt tcacaatgga
     6241 aaaatacctg cacctgttaa gtcggggaga taaaattggc ctgtcattga ttcgtctgag
     6301 tattgcgatt gtttttatgt ggattgggtt attaaagttt gtcccttacg aggcagacag
     6361 cattacacca ttcgtcgcaa acagtccact aatgtcgttc ttttatgaac acccggaaga
     6421 ctataaacag tatctgactc acgaaggcga atacaaacca gaagcaaggg catggcaatc
     6481 ggccaataat acctatggtt tttccaacgg tcttggcgtc gtggaggtga ttattgctct
     6541 gctggttttg gctaatcctg tcaatcgctg gttaggttta ttgggaggac tgatggcata
     6601 taccacgccg ctggtaacac tctcattttt aatcaccacc ccggaggcat gggtacccgc
     6661 attgggtgac gctcatcatg gtttccctta tttatccggt gctggtcgct tggtattgaa
     6721 agatactctg atgctggcag gtgctgtcat gataatggca gattcggcgc gggaaattct
     6781 taaacaacgc agtaatgaat ccagttcaac gttaaaaact gaatattgat aatccgaaca
     6841 ctgtctgttg ttcacttttt caggcgtcag gccccctttc tgatgatgaa gcctgacgcc
     6901 atattaccgg agatatgaca gagtgaagat caatatcact cccctgttaa aagattcttg
     6961 tcgtctcagg taaaatatat gattaatatc aatcatgtat ttttacagca agacaaccac
     7021 gaacaaattc aggattaaaa tgttttaccg actccccaac ataacccaaa tcaagcgaac
     7081 taatttgtgc catttctttc tctgtcagtg agaaatccca gatagcaaaa ttttcttcga
     7141 tacgctgttg atgtgtcgat ttcggaataa cgaccactcc ccgttgaata ttccaacgca
     7201 gaatgacttg agaaatcgat ttttgatggg catctgcaat actctgaagc agattatttt
     7261 caaagggttt atgtcgtcca ccacctaacg gagcccatgc ttcaggctgc acgttataat
     7321 acttcatggt tgccagcgcc tctggttggg caaaataggg atgcaactcg acctggttga
     7381 ccatcggttt aactcttacc gtttcacaaa agttcgccag aacatgagga tagaaattgg
     7441 aaaccccaat tgcttttaat ttgccctctt cataggcatc ttcgagtgca cgccacacac
     7501 tgaaataatc gcgcatagcc tggtgcaata aatagaggtc gaagtactct agtcccgatt
     7561 tttttaatga tgcttcaata cctgctgctg ccgtatcctg attcaacata tcctgccccc
     7621 acaatttaga cgttataaat aactcatctc gggtacataa accttcagat atagcttcac
     7681 gaacggcttc accgacggca tcttcattac cgtataccgc tgcagtgtcg atgagtcgat
     7741 aacccgtgcg gatagcgttc aacactgact gtttgcacac gtttttatcg gtaacctgaa
     7801 aaacaccaaa tcccatcatc ggcattttca gattgttact taatacggaa aattccactg
     7861 tcagtccctc cattaggcat gaacaatgag tctacgttaa aacataacct caaattagta
     7921 tgtggatttt gatatcactt atgcaaaaaa ttcattaata atgtaggact gaaacctctc
     7981 tattttcggg gacaacgaag cagacgctac cagtgctttt gccttcgacc ttgctgtttt
     8041 tgatacactt agggccttgg gcataacgaa aatgtgcgat atcacgaaga attaatggag
     8101 aatg
//
LOCUS       DADMKF010000170         8027 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.173, whole genome shotgun sequence.
ACCESSION   DADMKF010000170 DADMKF010000000
VERSION     DADMKF010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8027
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.173"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            812..1054
                     /locus_tag="JNC24_003509"
     CDS             812..1054
                     /locus_tag="JNC24_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9160176.1"
                     /translation="MNSLAGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
     gene            complement(1384..2313)
                     /gene="fdhE"
                     /locus_tag="JNC24_003510"
     CDS             complement(1384..2313)
                     /gene="fdhE"
                     /locus_tag="JNC24_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HAY9160177.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQEVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(2310..2945)
                     /gene="fdoI"
                     /locus_tag="JNC24_003511"
     CDS             complement(2310..2945)
                     /gene="fdoI"
                     /locus_tag="JNC24_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HAY9160178.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHHNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(2942..3844)
                     /gene="fdxH"
                     /locus_tag="JNC24_003512"
     CDS             complement(2942..3844)
                     /gene="fdxH"
                     /locus_tag="JNC24_003512"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit beta"
                     /protein_id="HAY9160179.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(3857..6907)
                     /gene="fdnG"
                     /locus_tag="JNC24_003513"
     CDS             complement(3857..6907)
                     /gene="fdnG"
                     /locus_tag="JNC24_003513"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(6320..6322),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HAY9160180.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNREYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            7101..7934
                     /gene="fdhD"
                     /locus_tag="JNC24_003514"
     CDS             7101..7934
                     /gene="fdhD"
                     /locus_tag="JNC24_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HAY9160181.1"
                     /translation="MKKTQRKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
BASE COUNT         1865 a         2064 c         2085 g         2013 t
ORIGIN      
        1 atttacatca ttccctgtct atgccggtaa tattgaataa gaactctata aatttttatt
       61 cttcgttggc accatacgtg tcttgctaca tcactgttca ggagcattta aatattattt
      121 gtagagctga gtggcaatta tagcagataa tgcttaactc atcatcttag ttcaggtttg
      181 agcgattttt gatagtcaaa acctcctctg gtgccatact tttctcataa atatttttaa
      241 aatctatagc tcacaaacat tatgcaaaga taatgggctg gcatcattgt cagtatcatt
      301 catttactgc tctgtatgtt atgaatcatt atgttgtatg tttgctctta aaccgtggga
      361 aggaggctat aatggttgcc aatattaatc tcattaaaca agaaagttat tctgttgtta
      421 atttagaaaa acaactttcc cgaactgtta ccaacaaaat tataacgatg agcaagtaaa
      481 gaccggttgg tggcagtact gaccggtctg tgatgaaaga ttaatttcac cttaaatcct
      541 ggtgctgtta ggatgctgac gtttttcggc tacaccattc agattatgca aataaatatc
      601 cctgaatata atatattcat agcacccata actcatcctg ggcgataact aaaccatttt
      661 tgagtaataa atgaaacaac tgcaacgctc ttattatttc tggaaaacgc ctcgcaaaaa
      721 taaaaaatga tgcgtaaatg agctttcttt aaagattcgg caagggtact tttctctcca
      781 tagattcaaa aatgatattg gcgagatatt tatgaactct ctggcaggta tagatatggg
      841 cagaatttta ctcgatttat cgaatgaggt gattaagcaa cttgatgatc tcgaggtgca
      901 gcgtaatctt cctcgtgcgg acctattaag ggaagcagta gatcaatacc tgataaatca
      961 atcgcaaaca gcaagaacca gtgttcctgg catctggcaa gggtgtgagg aagatggtgt
     1021 cgaatatcag cgtaagctgc gcgaggaatg gtaatgggaa aaaatgatgt caatcagatt
     1081 gctgacaacg tgcgcgttgt tcatgccgga tgcggcgtga acgccttatc cggcctacaa
     1141 agtcatgcaa attcaatata ttgaagttta ttggtaggcc tgataagcgc agcgcatcag
     1201 gcaatcatac gcttgtcatc agtgacatac ccataacctt atcgatgtgt tggagggaag
     1261 aacgaggcaa acagacaatt gcggcatgat cttgaatggg taacgcggaa tacgagagat
     1321 ggtgtcgtca caccctatga gctgtaatcc gggcgacctg cgccacccgg aaaacgtcag
     1381 agattactct ccttcacccg gaaacaggaa cgggttaatg gaactacggg cgtagccttc
     1441 ttgctccatt cgcgcgtcca gtaccagaga ggcgaggtca tctgccacgg cttcaacttt
     1501 cggttctttt tcctgataaa gaatcttcag gtaagtgccg cagtcatcgc agctttcggc
     1561 tttaatcgcg gcctgttcgt catccagcga ccagtaatgc agtttgccgc tctgctcgca
     1621 gttgctgcat tttacgcgca ctacgtgcca ttcggtttca cacaggttgc agtgcaggta
     1681 acgcagaccc tgagtggtgc caatttgcac catgctggac accggcatag agccacatac
     1741 cgggcaatat tgacgttgtt cgccgtattc agcgcgggct ttgccgggga tcagattggc
     1801 catctgcgcc cagtagagcg acagcgcagc ccagataaac ggcgctttat cgctgctgac
     1861 ggacgagaaa tcagaggcaa acagcgcgct ggccatatct tccagctcct gagtcgatgc
     1921 cttctccaga ttctcaatca ctgccagcgc cgggccgctc atttcaggtt tcagctcagc
     1981 aattagcgcc atcagcagct tttgccagtg cttatcacgc ggcagaacgt gaatatccag
     2041 cgggggcttg ccctgtgcgc tggcttcttt aatgcgcgcg gtcagatcca tctccagcgg
     2101 atggtcgtac agcaccactt cctgggcgtg ggcgataagc gcagcaaagc gcaggtaatc
     2161 acccagcgga ttattttctg ccagctcgcg cagacgctcg gcgcggcggt tgtataaatt
     2221 cttgagccga gggaacaata acggcggaat catatccgcc gtacgtttct cgctcgaacc
     2281 cagctcatct tgcgggatta tgcgaatact cattcagctt tcttttccgt tgtcttgcgg
     2341 acctcacggt accagcgcgg gtgatgtttc ttcgcccagg cgctggtaac ccatccttcc
     2401 accatggcgg taatcgtgcc tttcacccaa agggcggcgt agatatgcac catgataacc
     2461 acaattaacg ccactgcggc aaatgaatgc agcattaacg cgaatcggat caccgggatt
     2521 gagaaagcag gcgcaaaata aggacgccag ataatcacgc cgctcaccag cagcagaacc
     2581 aggaaaataa tcgccgccca gaaaacgcat ttctgaccga agttataacg cccggtgtca
     2641 cctacttcct cgttgacgac gatcttacga atattcttcg cccaaaagat atcatcccga
     2701 ttgattaggt tgtgatgcca gtaacggaaa aacatgatga tgaacgaggc aaacataacc
     2761 acgccgacaa acgggtgcag aattcgcgcc agctgcggtg tgcccatgat ttgcatcaac
     2821 cagttgaagg acgggaacaa aaagcccagc ccgctcaccg ccgccaggat gaagcagaag
     2881 gcggtgatcc agtggttgat acgttccggc gcggtgtagc gcacgatggt gtcacgtcgt
     2941 ttcatttgcg ctcctcgtct ttctcttcgt gcagattatt ctcttcctca tccgcacggt
     3001 tcggaccgac acccacgtag tggaagatac tggccgcaaa ggtagccgca aagccaacag
     3061 ccgcgagcgg tttccagatg cctttccaga atttcacggt ttcgctgatt tccgggttct
     3121 ccggcaagcc atgatacaga tttggcttgt cagcatggtg cagcacgtac atgacgtgtg
     3181 taccaccgac gcctgccgga tcgtacagac ccgcattgtc gtaaccgcgg gttttcagct
     3241 cagcaacgcg ctcgctcgcc agcgttttca tcgactcttt cgtaccgaag tgaatcgcgc
     3301 ccgttgggca ggtcttcacg caggctggtt cttgcccaac caccacgcgg tcaacgcaca
     3361 gcgtacattt gtagacgcgg ttgtcttccg ggttgaggcg cggaatgtcg aacggacagc
     3421 ccgcaatgca ataaccgcag ccaatgcact gctcggactg gaagtcgacg ataccgttgg
     3481 catactgaat aattgcacct tccgccgggc acgctttcag gcagcctgga tcggaacagt
     3541 gcatacagcc gtctttgcgg atcagccatt ccagtttgtc gttctgctcc acttccgaga
     3601 agcgcatcac cgtccacgat ttggcgctta aatcattggg gttgtcgtac accccaatgt
     3661 tattgccgac ggtatcgcga atgtcgttcc actctgaaca cgccacctga caggctttac
     3721 agccgataca ggtggtgacg tcgatgagtt tcgccacttc ttcctggaag tcccgcgcct
     3781 gaggcgcggg ggtcagaccg ttagtcgcgg aacgacgaat gatatcttgc gattgataag
     3841 ccatatgtcg tctccgttac accttttcca cattcacaag gaaggactta aactccggcg
     3901 tctgcgtgtt cgcatcacca acgaatggcg ttaacgtatt ggcaataaag ccttttttag
     3961 ctacgccttc atagccccag tgaatcggaa taccgatggt atcgatatct ttgccgttcg
     4021 ctttcagcgt gcgaatacgt ttggtcacca ccgctttggc tttgatatag ccacggttgg
     4081 aggagacttt cacggtatcg ccctgggcaa tgccaagttt attcgccagc gactccccga
     4141 tttccacaaa ctgctctggt tgcaaaatcg cgttcaacag cgcgtgtttg gtccagtagt
     4201 ggaagtgctc ggtcagacga taggtggttc cgacatacgg gaacttatcg gctttaccca
     4261 atgcttcggc gtcgtcttta aagattcgcg cagccgggtt cgagataacg tttggatgca
     4321 gcgggttagt tcccagcggc gtttcaaacg gctcgtagtg ttccgggaac ggaccttccg
     4381 ccatcttatc gagggcaaac agacgcccca tgccttcctg ctgcatgata aacggcccga
     4441 cgccgctgcc cggaggcgct gcgctgtagt ccggaatatc ccagccggtc cacttagtgc
     4501 cgtcccattt cagcaactga cgcttcggat cccacgggtt accctgcgga tctgcggagg
     4561 cgcggttata cagaatgcgg cggttaagcg gccatgccca tgcccagccc agcgtgttac
     4621 cgaggccaga cggatcggcg ttatcacgac gcgccatctg gttgccttcc ggcgtccagc
     4681 taccggcgaa aatccagcag ccacaggagg ttgtaccgtc atcgcgcaat tgggcgaacg
     4741 agctaagttg ttggcctttc ttgacgataa ccgcaccggt tgccggatcg gtaatatcgg
     4801 ccagcgcctt accgttgctc tccatcgcca cttcttccga cttcggctca tgcggaatgg
     4861 cgtagttcca ggtcatgttc agcacctgat ccgggtttgc gccaccctgt tcggcataca
     4921 tcttgcgcaa gcgcaggaag ataccggaga ggatctcgcc atcagtcagc gcaatccctg
     4981 gggcgtccgc acctttccag tgccactgca accagcgacc tgagttaacg attgaaccgt
     5041 tctcttctgc gaagcaggtc gatggcagac ggaacacttc ggtctggatc ttcgacgagt
     5101 caacttcgtt cagctcaccg tggttctgcc agaagttaga ggtttcagtg ttcagcgggt
     5161 cgatggtgac gaggaacttc agtttcgaca gacaaccgat cactttgttt ttgttcggga
     5221 atgaggcaac agggttaaag ccctggcaga tatagccatt gaccttgccc tctttcatca
     5281 tctcgaagta ttgcaggacg tcgtagcctt tatcccactt cggcaaccag tcaaagcccc
     5341 agctattttc cgccgtcgct ttatcaccaa agaaggcctt catcatagag acgaagaatt
     5401 tcgggtagtt gccccagtag tttacctggc cttccagcag cggttttggc gtgttggcgg
     5461 taaggtaggt ttgcagatcg gtctgcttct cgcttggcag cgtcatgtaa cctggcaggc
     5521 tctgcgacag cagccccagg tccgtcagcc cctgaatatt ggagtgaccg cgcagggcgt
     5581 taacgccgcc gcctgccatc cccatattgc cgagcagcag ctggatcatc gccatcgtac
     5641 gaatgttttg cgcaccaacg gagtgttgcg tccagccgag ggcatacagg aacgaggcag
     5701 ttttatcgtg agcactggtt tctgcgatgt attcgcagac tttcaggaac gcgtcttttg
     5761 gcgtaccaca gatgttttca accacatctg gcgtgtagcg ggaaacgtgc tgtttcagca
     5821 agttccacac gcagcgcgga tgttgcagcg tggtatcgcg tttggcgaag ccgttttcgt
     5881 ccagttcgta agtccaggag gatttatcgt acttgcgttt ttccgcgtca tagccggtga
     5941 acaggccatc ttcaaagccg taatcctcac gcacgatcag gctggcgttg gtataggctt
     6001 cggtgtattc gcggttgaat ttttcattgt tcagcaggta cagcaatacg cctgacagga
     6061 aagcaatgtc agtaccggaa cgaatagggg catagtagtc agccaccgcc gccgtacgcg
     6121 taaagcgagg atcgatcaca atcagcttcg cgccattgtg aattttggct tccatcgccc
     6181 agcggaaccc gaccgggtga gcttcagcgg cgttaccgcc catcaccacg acgaggttgg
     6241 cgttcttgat gtcgacccag tggttggtca tcgcaccgcg accaaatgtt ggagcaagac
     6301 ttgctaccgt tggtccgtgt cagacacgcg cctggttgtc gaccgcgagc atacccagcg
     6361 cgcgggagaa tttttgcgtt aaatagccgg tttcgttact cgacgcggaa gcacacagca
     6421 tcccggtgga gagccagcgg ttaacagtca cgccttcggc gttttgcgca atgtagttag
     6481 catcgcggtc ttctttcatc agtttggcga tgcgatcaaa cgcctcttcc caactgattt
     6541 gctgccattt atcagaacct ggcgcacggt attccggaaa cttcagacgg ctttcggagt
     6601 ggatgaaatc caccaggcca gcgcctttcg gacaaagtgc accgcggttg accgggtgat
     6661 ccggatcgcc ttcgatatgg aagatagatg ctttggcgtt ttttgctccg tcaccgaggc
     6721 tgtacatcaa cagcccacag cctacggaac aataggtgca ggtattacgg gtttcgcggg
     6781 tgcgcagcag tttatactgc cgggtttccg cgagtgctac gctgggtgca aaacccagtg
     6841 ccgctgccgt ggtgcctgcc ataccgccag cgcagatctt aaagaactgc cttctgctga
     6901 cctgcatgga ttgctccttg tttcgacatt gtcacgtccc atttacattc gcttgctgcg
     6961 tgtgcaggga gtgggagtta tttttctttg cggaaggggc cgcaaaggtc cagaattggc
     7021 tcaatttccc tccatccagg aagggtttgt aacagaatac cataatgttg gtgtgtgtgt
     7081 tcttatctgg ttaagagaaa gtgaaaaaaa cacagcgaaa agaaatcgaa aatgtgacaa
     7141 atatcacagg tgttcgtcaa attgagttat ggcggcgtga tgatttacaa cacccacggt
     7201 tagatgaggt cgcggaagaa gttcccgttg cgctggtcta caacggcatt tcgcatgtgg
     7261 tgatgatggc gtcgcccaaa gaccttgagt actttgcgct cggtttttcg ctttccgagg
     7321 ggataataga aagtccgcgc gatatcttcg gcatggatgt cgttccttcc tgtaatggtc
     7381 ttgaagtaca aattgagctt tccagccgcc gctttatggg gttgaaggag cgccgccggg
     7441 cgctggcggg acgtacgggc tgtggcgtat gcggcgtgga gcaacttaat gacatcggaa
     7501 aaccggtgca gccgctaccg ttcacccaga cgtttgatct caacaaactg gatgatgcat
     7561 tacgtcatct caacgatttt cagccagtgg ggcaactgac tggttgtact cacgccgctg
     7621 cctggatgtt gccatctggc gaactggtcg gcgggcatga agacgtgggt cgccatgtgg
     7681 cgctggacaa actgttaggc cgccggtcac aagaagggga aagctggcag caaggtgcgg
     7741 tactggtttc cagtcgtgcc agttatgaaa tggtgcaaaa gtcggcgatg tgcggcgtag
     7801 agattttgtt tgcggtgtct gccgcgacca cgcttgctgt agaagtggct gagcgctgta
     7861 atctgacact ggtaggtttt tgtaaaccgg gtcgggcaac ggtttatacc catccgcagc
     7921 gtttgagcaa ttaattaaaa taagtcctgc gatatatatt aaattgcagg acttattcat
     7981 ttcgtgaatt ttattatttt atttataaat aaaacgcgtg tagtgac
//
LOCUS       DADMKF010000171         7968 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.174, whole genome shotgun sequence.
ACCESSION   DADMKF010000171 DADMKF010000000
VERSION     DADMKF010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7968
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.174"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            6..239
                     /locus_tag="JNC24_003515"
     CDS             6..239
                     /locus_tag="JNC24_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001338296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HAY9160182.1"
                     /translation="MAHTEINVNQTNHVGWTPLLEAIVLNDGGIKQQAIVQLLLEHGA
                     SPHLTDKYGKTPLELARERGFEEIAQLLIAAGA"
     gene            279..1142
                     /locus_tag="JNC24_003516"
     CDS             279..1142
                     /locus_tag="JNC24_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HAY9160183.1"
                     /translation="MWALTADADFLAQRGQGQVEQVFARAVNIALPARQQLLTLLCEE
                     YDNAPNSCRLALTHFDDLFRHGDKVQFDDQGITVGQHLHIEMSRCRRWLSPTLQMTAV
                     NFHLIAWLQWHDIIHQHLGENETLFNYRGDNPFYQALNKELHIKRRAVIQTVNDKQNI
                     AAAVASMIGLGIGLTPSADDYLTGLALILFIPGHPAEKYKEEFYRGLLRGRNNTTLLS
                     AITLEAALQQRCRENIHRFIHNIIYGVPGNSTQAIEKIKHIGSSSGCDMLYGMADGCA
                     LSQTYGGNYVS"
     gene            1132..2679
                     /gene="fdrA"
                     /locus_tag="JNC24_003517"
     CDS             1132..2679
                     /gene="fdrA"
                     /locus_tag="JNC24_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HAY9160184.1"
                     /translation="MSVKIVIKPNTYFDSVSLMSISTRANKLDGVEQAFVAMATEMNK
                     GVLKNLGLLTPTLEQAKNGDLMIVINGKSGADNEQLLVEIEELFNTKAQSGSHEARYA
                     TIASAKKHIPESNLAVISVNGLFAAREARQALQNDLNVMLFSDNVSVEDELALKQLAH
                     EKGLLMMGPDCGTAIINGAALCFGNAVRRGNIGIVGASGTGSQELSVRIHEFGGGVSQ
                     LIGTGGRDLSEKIGGLMMLDAIGMLENDPQTEIIALISKPPAPAVARKVLERARACRK
                     PVVVCFLGRVETPVDEQGLQFARGSKEAVLKAVMLSGVKQENLDLHTLNQPLIADVRA
                     RLQPQQKYIRGLFCGGTLCDETMFAVMEKHGDVYSNIQPDPEFRLQDINRSIKHTFLD
                     FGDDDFTNGKPHPMIDPTNRISRLIEEARDPEVAVIVMDFVLGFGSHEDPVGSTIEAI
                     KEAKAIAAAEGRELIILAYVLGTDLDTPSLEQQSQMLLDAGVILASSSTNTGLLAREF
                     ICKGEEA"
     gene            2679..4037
                     /locus_tag="JNC24_003518"
     CDS             2679..4037
                     /locus_tag="JNC24_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HAY9160185.1"
                     /translation="MSQSLFSQPLNVINVGIAMFSDDLKKQHVEVTQLDWTPPGQGNM
                     QVVQALERIIQSHPVLIGFDQAINVVPGMTPKTILHAGPPITWEKMCGAMKGAVTGAL
                     VFEGLAKDLDEAAELAASGEITFSPCHEHDCVGSMAGVTSASMFMHIVKNKTYGNIAY
                     TNMSEQMAKILRMGANDQSVIDRLNWMRDVQGPMLRDAMKIIGEIDLRLMLAQALHMG
                     DECHNRNNAGTTLLIQALTPGIIQAGYSVEQQREVFEFVASSDYFSGPTWMAMCKAAM
                     DAAHGIEYSTVVTTMARNGVEFGLRVSGLPGQWFTGPAQQVIGPMFAGYKPEDSGLDI
                     GDSAITETYGIGGFAMATAPAIVALVGGTVEEAIDFSRQMREITLGENPNVTIPLLGF
                     MGVPSAIDITRVGSSGILPVINTAIAHKDAGVGMIGAGIVHPPFACFEKAILGWCERY
                     GV"
     gene            4180..5130
                     /locus_tag="JNC24_003519"
     CDS             4180..5130
                     /locus_tag="JNC24_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase family protein"
                     /protein_id="HAY9160186.1"
                     /translation="MKELVVVAIGGNSIIKDNASQSIEHQAEAVKAVADMVLEMLASD
                     YDIVLTHGNGPQVGLDLRRAEIAHEREGLPLTPLANCVADTQGGIGYLIQQALNNRLA
                     RHGEKKAVTVVTQVEVDKNDPGFAHPTKPIGAFFSESQRDKLQKANPDWCFVEDAGRG
                     YRRVVASPEPKRIVEAPAIKALIQQGFVVIGAGGGGIPVVRTEAGDYQSVDAVIDKDL
                     STALLAREIHADILVITTDVEKVCIHFGKPQQQALDRVDIATMTRYMQEGHFSPGSML
                     PKIIASLTFLEQGGKEVIITTPECLPAALRGETGTHIIKT"
     gene            5140..6522
                     /locus_tag="JNC24_003520"
     CDS             5140..6522
                     /locus_tag="JNC24_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HAY9160187.1"
                     /translation="MKESNSRREFLSQSGKMVTAAALFGTYVPLAHAAVAGTLNCEAN
                     NTMKITDPHYYLDNVLLETGFDYENGVAVQTRTARQTVEIQDGKIVALRENKQHPDAT
                     LPHYDAGGKLMLPTTRDMHIHLDKTFYGGPWRSLNRPAGTTIQDMIKLKQKMLPELQP
                     YTQERAEKLIDLLQSKGTTIARSHCNIEPVSGLKNLQNLQAVLARRQAGFECEIVAFP
                     QHGLLLSKSEPLMREAMQAGAHYVGGLDPTSVDGAMEKSLDTMFQIALDYDKGVDIHL
                     HETTPSGVAAINYMVETVEKTPQLKGKLTISHAFALATLNEQQVDALANRMAAQQISI
                     ASTVPIGTLHMPLKQLHDKGVKVMTGTDSVIDHWSPYGLGDMLEKANLYAQLYIRPNE
                     QNLSRSLFLATGDVLPLNEKGERVWPKAQDDASFVLVDASCSAEAVARISPRTATFHK
                     GQLVWGSVAG"
     gene            6899..7948
                     /gene="yahK"
                     /locus_tag="JNC24_003521"
     CDS             6899..7948
                     /gene="yahK"
                     /locus_tag="JNC24_003521"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase YahK"
                     /protein_id="HAY9160188.1"
                     /translation="MKIKAVGAYSAKQPLEPMDITRREPGPNDVKIEIAYCGVCHSDL
                     HQVRSEWAGTVYPCVPGHEIVGRVVAVGDQVEKHAPGDLVGVGCIVDSCKHCEECEDG
                     LENYCDHMIGTYNSPTPDEPGHTLGGYSQQIVVHERYVLRIRHPQEQLAAVAPLLCAG
                     ITTYSPLRHWQAGPGKKVGVVGIGGLGHMGIKLAHAMGAHVVAFTTSEAKREAEKALG
                     ADEVVNSRNADEMAAHLKSFDFILNTVAAPHNLDDFTTLLKRDGTMTLVGAPATPHKS
                     PEVFNLIMKRRAIADSMIGGIPETQEMLDFCAEHGIVADIEMIRADQINEAYERMLRG
                     DVKYRFVIDNRTLTD"
BASE COUNT         1919 a         2018 c         2308 g         1723 t
ORIGIN      
        1 agcttttggc gcatacggag attaacgtta accagaccaa ccatgtaggc tggacgccgc
       61 tactggaagc gattgtgctt aatgatggtg gtattaaaca gcaggcgatt gtgcagttat
      121 tgctggaaca cggtgccagc ccgcatctga ccgataaata tggcaaaacg ccactggaac
      181 tggcgcggga acggggcttt gaagagattg cgcagttact gattgccgca ggtgcataaa
      241 ccgggaggct ggctatcaac acaccagaaa aacggtgtgt gtgggcgcta actgcggatg
      301 cggattttct ggcgcagcgg gggcaaggac aggttgaaca ggtctttgcc agagcggtaa
      361 atatcgcact cccggctcgc cagcagttgc tgacgctgct ttgtgaagag tacgacaatg
      421 cgccaaacag ttgtcggttg gcactcactc actttgatga tctgttccgg catggtgata
      481 aggttcagtt tgacgatcaa ggtattacgg ttggtcaaca tcttcatata gagatgagtc
      541 gttgtcggcg ttggctgtcc ccaaccttgc aaatgaccgc tgtgaatttt caccttatcg
      601 cctggctaca gtggcacgac attattcatc agcacctggg ggaaaatgaa accctgttta
      661 attatcgcgg cgataatccg ttttatcagg cgttaaataa agaattacat attaaacgac
      721 gggcagttat tcagacagta aacgataagc aaaatatcgc cgcagcggtc gccagtatga
      781 ttgggttagg gattggcctt acgccatcag ccgatgatta tttaacaggt ctggcgctta
      841 ttttatttat tcccgggcat ccggcagaaa aatacaaaga ggaattttat cgcggtctgc
      901 tacgcggcag aaataacacc acgttattaa gtgccataac gctggaagcc gcattacaac
      961 aacgctgccg ggaaaatatt catcgcttta ttcacaacat tatttatggc gtccctggga
     1021 attcaactca ggcaatagaa aaaattaaac atattggctc cagttccggc tgcgacatgc
     1081 tgtatggcat ggccgatggt tgtgcgctga gccaaaccta cggagggaat tatgtcagtt
     1141 aaaatagtca ttaaaccgaa tacctatttt gattctgtct cgctgatgtc tatctccacg
     1201 cgtgcaaata aactcgacgg cgtcgagcag gcatttgtgg cgatggcgac cgaaatgaac
     1261 aaaggtgtgc tgaaaaattt aggactgctg acgccgacgc tggaacaggc gaaaaacggc
     1321 gacctgatga ttgtcatcaa tggtaaatcg ggtgcggaca acgagcagtt gctggtggag
     1381 attgaagaac tgttcaacac caaagcgcaa agcggctcgc acgaggcgcg ttacgccact
     1441 attgccagcg ccaaaaagca tatcccggaa agtaacctgg cggtgatttc ggtcaacggt
     1501 ctgtttgccg ctcgcgaagc gcgtcaggcg ctgcaaaacg atctcaacgt gatgctgttt
     1561 tccgataacg tctcagttga agatgaactg gcgctcaagc agttggccca cgaaaaaggg
     1621 ctgctgatga tggggccgga ctgcggcacg gcgattatta acggcgcggc gctctgtttt
     1681 ggtaacgccg tgcgtcgcgg caatatcggt attgttggcg catccggcac cggcagccag
     1741 gagctgagcg tccgcattca tgaatttggt ggcggtgttt cacaactgat tggcaccggc
     1801 gggcgcgacc tgagcgagaa aatcggcggc ctgatgatgc tcgacgccat cgggatgctg
     1861 gaaaacgatc cgcaaactga aattatcgca ctgatttcaa aacctcccgc gcctgcggtg
     1921 gcccgcaaag tgctggaacg cgcacgtgcc tgccgcaagc cggtggtggt ctgcttcctc
     1981 ggtcgcgttg aaacgccggt ggatgagcag gggctacagt ttgcccgcgg cagcaaagag
     2041 gcggtgctaa aagcggtgat gctctccggc gtgaaacagg aaaatctcga cctgcatacg
     2101 cttaaccagc cgttgattgc tgatgtgcgt gcgcgtctgc aaccgcagca gaaatacatt
     2161 cgtggcctgt tctgcggcgg cacgctgtgc gacgaaacca tgttcgcggt gatggaaaaa
     2221 catggcgatg tctacagcaa cattcagccc gatccggaat tccgcctgca agatatcaac
     2281 cgcagcatca aacacacctt cctcgacttt ggcgatgacg acttcacgaa cggcaagccg
     2341 catccaatga ttgaccccac caaccgcatc agtcgcttga tcgaagaggc gcgcgatcca
     2401 gaagtggcgg tgatcgtgat ggattttgtg ctcggatttg gatcgcatga agatccggtc
     2461 ggctccacca tcgaggcgat caaagaagcg aaagcgatcg ccgctgccga aggacgcgag
     2521 ttgatcattc tcgcctatgt gctgggtacc gatcttgata cgccatcgtt agaacaacaa
     2581 agccagatgc tgcttgatgc cggagtgatt ctggcgagca gcagcaccaa taccggattg
     2641 ctggcgcgtg aatttatctg caaaggggag gaagcctgat gagccagtca ctgtttagcc
     2701 aaccattgaa cgttattaac gtcggcatcg ccatgtttag cgatgacctg aaaaagcagc
     2761 atgtagaagt gactcaactc gactggacgc cgccggggca gggcaatatg caggtggtgc
     2821 aggcgctgga gcgcattatc cagtcgcatc cggtgctgat tggttttgat caggcgatta
     2881 acgtggtgcc gggcatgacg ccgaaaacca ttcttcacgc cgggccgccg atcacctggg
     2941 aaaaaatgtg cggcgcgatg aaaggcgcgg tcaccggagc gctggtgttc gaaggactgg
     3001 cgaaagatct cgacgaggcg gctgaactgg cggcctccgg ggagatcacc ttctcgccgt
     3061 gccacgaaca cgactgtgtg ggatcgatgg cgggcgttac ctcggcctcg atgtttatgc
     3121 acatcgtgaa aaacaaaacc tacggcaaca tcgcttacac caatatgagc gagcagatgg
     3181 cgaagatttt gcgtatgggt gccaacgatc agagcgtgat cgaccgcctc aactggatgc
     3241 gcgatgtgca ggggccaatg ctgcgcgacg cgatgaaaat tatcggtgag atcgatctgc
     3301 gcttaatgct ggcgcaggcg ctgcatatgg gcgatgagtg ccataaccgc aataacgccg
     3361 ggacgacgct gctgattcag gcactgacgc cggggatcat tcaggcaggc tattccgtcg
     3421 agcaacagcg cgaagtgttc gagtttgtcg ccagcagcga ctacttctcc ggcccgacgt
     3481 ggatggcgat gtgtaaagcg gcgatggatg cggcgcacgg catcgaatac agcaccgtgg
     3541 tcaccaccat ggcgcgtaac ggcgtcgagt tcggcctgcg ggtcagcggc ctgccggggc
     3601 aatggtttac cggcccggcg cagcaggtga tcggcccgat gtttgccggt tataagccgg
     3661 aagattcggg gctggatatc ggcgacagcg ccatcaccga aacctacggt attggtggat
     3721 ttgctatggc gaccgcgcct gcaatcgtcg cgctggtggg cggcacggtg gaagaggcta
     3781 tcgatttctc ccgtcagatg cgtgaaatca ctctcggtga aaaccccaac gtcaccattc
     3841 cgctgctcgg ttttatgggc gtgccgtcgg caatcgacat cacccgcgtg ggtagcagcg
     3901 gcattctgcc ggtgatcaac accgccatcg cccataaaga tgcgggcgtc ggcatgattg
     3961 gcgcgggcat tgtgcatcca ccattcgctt gcttcgagaa agccattctc ggctggtgcg
     4021 aacgttacgg cgtctgactt acatgtttat gccggatgcg gcgtgaacgc cttatccggc
     4081 ctacggatgg cccgagaatt gtaggcctga taagcgtagc gcatcaggca gtctggcgtt
     4141 ggtcataacc tcatcaccct ctgtagcgga cataacaaca tgaaagagct tgtggtcgtt
     4201 gccattggtg gcaacagcat tatcaaagat aacgccagcc agtcgattga gcatcaggcg
     4261 gaggcggtga aagccgtggc cgatatggtg ctggaaatgc tggcttccga ttacgacatt
     4321 gtgctgaccc acggtaacgg gccgcaggtc gggctggatt tacgccgtgc ggagattgcc
     4381 cacgagcgcg aagggctgcc cttaacgccg ctggcgaact gtgtggcaga tacgcagggc
     4441 ggcatcggct atctgatcca acaggcgctg aataaccgac tggcgcgtca cggcgagaaa
     4501 aaagccgtca ccgtggtaac tcaggtggaa gtggataaaa acgatccagg ttttgcccat
     4561 cccaccaagc ccattggtgc attcttcagt gaaagccagc gtgacaaact acaaaaggca
     4621 aaccctgact ggtgttttgt tgaagatgcc ggacggggct atcgccgcgt ggtcgcctcg
     4681 ccggaaccga aacgtattgt cgaagcaccc gccattaagg cgctgatcca acaaggtttt
     4741 gtcgtaattg gcgcgggcgg cggtggaatt ccggtagtgc gtactgaagc gggggattac
     4801 caaagcgtgg acgcggttat tgacaaagat ctctctaccg cgctgctggc ccgtgaaatt
     4861 cacgccgaca ttctggtgat caccaccgac gtcgaaaaag tctgtatcca ctttggtaaa
     4921 ccgcagcagc aggcgctgga tcgggtggat attgccacca tgactcgcta tatgcaggaa
     4981 gggcattttt ctcccggcag catgttgcca aaaatcatcg ccagcctgac gttcctggag
     5041 cagggcggca aagaagtgat tatcaccacg ccggaatgcc tgcctgcggc gctgcgcggt
     5101 gaaacgggca cccatattat taaaacgtaa ggacgtagga tgaaagaaag caatagccgc
     5161 cgtgaatttc tgagccagag cggtaagatg gtcaccgccg ccgcgctgtt tggtacctat
     5221 gtgccgctcg cccatgcggc ggtagctggc accctaaact gcgaagcgaa caacaccatg
     5281 aaaatcactg acccgcatta ctatctcgat aacgtgctgc tggaaaccgg ttttgactac
     5341 gaaaacggtg tggcggtgca aacccgcacg gcgcgccaga ccgtggagat tcaggacggc
     5401 aaaattgtcg ccctgcgcga gaacaagcag catccggacg ccacgctgcc gcactatgac
     5461 gctggcggta agttgatgct acccaccact cgcgacatgc atattcatct cgacaaaacc
     5521 ttctacggcg ggccgtggcg ctcgctcaat cgtccggcag gcaccactat ccaggacatg
     5581 atcaaactca agcagaaaat gctgccggag ttacaaccgt atacgcagga gcgggcggaa
     5641 aaactgatcg atctattgca gtcgaaaggc accaccattg cccgcagcca ctgcaatatc
     5701 gaaccggttt ccggcctaaa aaatctgcaa aatttgcagg cggtgctggc gcggcgtcag
     5761 gcgggcttcg agtgtgaaat cgtcgccttc ccgcagcacg gtttgctgct gtcgaaatcg
     5821 gaacccttaa tgcgcgaagc gatgcaggcg ggtgcgcatt acgtcggcgg gctggacccg
     5881 accagtgtcg atggcgcgat ggaaaaatcc ctcgacacca tgtttcagat tgcgctggac
     5941 tacgacaaag gtgtcgatat tcacctgcac gaaaccactc cgtcgggcgt ggcagccatc
     6001 aattatatgg ttgaaacggt agagaaaacg ccgcaactga aaggtaagct gaccatcagc
     6061 cacgcctttg cgctggctac gctcaacgaa caacaggtcg atgcgctggc gaatcggatg
     6121 gcggcgcagc aaatttctat cgcctcgacg gtgccgattg gcacgctgca tatgccgctc
     6181 aaacagttgc acgacaaagg cgtaaaagtg atgactggca ctgacagcgt tatcgaccac
     6241 tggtcgcctt atggtctggg cgacatgctg gaaaaagcca atctctacgc gcagctctat
     6301 attcgtccta acgaacagaa cctctcccgt tcgctgtttc tagccactgg cgatgtattg
     6361 ccgctgaatg aaaaaggcga gcgtgtatgg ccaaaagcgc aggatgatgc cagctttgtg
     6421 ctggtggacg cctcctgttc cgccgaggcg gtggcgcgta tctcgccgag aaccgcaacg
     6481 ttccataaag ggcaactggt gtgggggagt gtggcaggtt gatgcgcatt tgaaacgcct
     6541 gcaacggtga attgtaagag atttaagccc cagggagtgg ggcgacctca atatgatagt
     6601 gtcggcagaa atggacatta tctttgagga atatgccctt attgacgttg tgtagataac
     6661 tatttgtcta cgtgaaaaga tcatcagttt tgccgatttt agtcttttac ggatagcaaa
     6721 tatcacactt ataggcgctc gccttatggg gaacgttgct gctctgtttc attcaggttc
     6781 gcggcatgtt ggggctggat cgggtggttt aaatattggc tatattcaat ggacgcgttt
     6841 tgccgcgatg acatatcagg cgtcgccaaa tacacatagc taatcaggag taaacacaat
     6901 gaagatcaaa gctgttggtg catattccgc taaacaacca cttgaaccga tggatatcac
     6961 acggcgtgaa ccgggaccga atgatgtcaa aatcgaaatc gcttactgtg gcgtctgcca
     7021 ttccgatctc caccaggtcc gttccgagtg ggcggggacg gtttacccct gcgtaccggg
     7081 tcatgaaatt gtggggcgtg tggtagccgt tggtgatcag gtagaaaaac atgcgccggg
     7141 cgatctggtc ggtgtcggct gcattgtcga cagttgtaaa cattgcgaag agtgtgagga
     7201 cgggctggaa aactactgtg atcacatgat cggcacctat aactcgccga cgccggacga
     7261 accgggccat actctgggcg gctactcaca acagatcgtc gttcatgagc gatatgttct
     7321 gcgtattcgt cacccgcaag agcagctggc ggcggtggct cctttgttgt gtgcagggat
     7381 caccacgtac tcgccgctac gtcactggca ggccgggccg ggtaaaaaag tgggcgtggt
     7441 cggcatcggc ggtctgggac atatggggat taagctggcc cacgcgatgg gggcacatgt
     7501 ggtggcattt accacttctg aggcaaaacg cgaagcggaa aaagccctgg gggccgatga
     7561 agttgttaac tcacgcaatg ccgatgagat ggcggctcat ctgaagagtt tcgatttcat
     7621 tttgaataca gtagctgcgc cacataatct cgacgatttt accaccttgc tgaagcgtga
     7681 tggcaccatg acgctggttg gtgcgcctgc gacaccgcat aaatcgccgg aagttttcaa
     7741 cctgatcatg aaacgccgtg cgatagccga ttctatgatt ggcggcattc cagaaactca
     7801 ggagatgctc gatttttgcg ccgaacatgg catcgtggct gatatagaga tgattcgggc
     7861 cgatcaaatt aatgaagcct atgagcgaat gctgcgtggt gatgtgaaat atcgttttgt
     7921 tatcgataat cgcacactaa cagattgaaa aaattaataa atacgctg
//
LOCUS       DADMKF010000172         7945 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.175, whole genome shotgun sequence.
ACCESSION   DADMKF010000172 DADMKF010000000
VERSION     DADMKF010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7945
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.175"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            <1..359
                     /locus_tag="JNC24_003522"
     CDS             <1..359
                     /locus_tag="JNC24_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cyclohexanecarboxylate-CoA ligase"
                     /protein_id="HAY9160189.1"
                     /translation="GRKKDIIVRGGENISSREVEDILLQHPKIHDACVVAMPDERLGE
                     RSCAYVVLKAPHHSLSLEEVVAFFSRKRVAKYKYPEHIVVIEKLPRTASGKIQKFLLR
                     KDIMRRLTQDVCEEIE"
     gene            complement(416..2794)
                     /gene="ppsA"
                     /locus_tag="JNC24_003523"
     CDS             complement(416..2794)
                     /gene="ppsA"
                     /locus_tag="JNC24_003523"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HAY9160190.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHEISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVEMMPDLPLKVMMNVGNPDRAFDFACLPNEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            3127..3960
                     /gene="ppsR"
                     /locus_tag="JNC24_003524"
     CDS             3127..3960
                     /gene="ppsR"
                     /locus_tag="JNC24_003524"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HAY9160191.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            4117..5163
                     /gene="aroH"
                     /locus_tag="JNC24_003525"
     CDS             4117..5163
                     /gene="aroH"
                     /locus_tag="JNC24_003525"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HAY9160192.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGAATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYAPRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTAIAGIMAESFLLEGTQKIVGSQPLTYGQSITDPCLGWEDTE
                     RLVEKLASAVDTRF"
     gene            5295..5486
                     /gene="hemP"
                     /locus_tag="JNC24_003526"
     CDS             5295..5486
                     /gene="hemP"
                     /locus_tag="JNC24_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HAY9160193.1"
                     /translation="MRYTDSRKLTPETDANHKAASPQPIRRITSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(5490..6926)
                     /locus_tag="JNC24_003527"
     CDS             complement(5490..6926)
                     /locus_tag="JNC24_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HAY9160194.1"
                     /translation="MTLSFVTRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNGAGVWGGEALLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TAEPGAMLMRVAPSHLRFGHFEHFYYRRESEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FSDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FITEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSTASPLRDEFIDRAAFDGW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMTELHRLHGAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(6989..7702)
                     /gene="ydiV"
                     /locus_tag="JNC24_003528"
     CDS             complement(6989..7702)
                     /gene="ydiV"
                     /locus_tag="JNC24_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HAY9160195.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQVLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLERDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRGLLSLSQVYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLDKSFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTAEILAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
BASE COUNT         2030 a         2086 c         1888 g         1941 t
ORIGIN      
        1 ccggacgcaa aaaagatatt attgtccgcg gcggcgaaaa tattagcagc cgtgaagtag
       61 aagatatttt attgcagcat cctaaaattc acgatgcctg tgtggttgca atgcccgatg
      121 aacgtttagg tgaacgatca tgcgcttatg tcgtgctgaa agcgccgcat cattcattat
      181 cgctggaaga ggtggtggct ttttttagcc gtaaacgggt cgcaaaatat aaatatcctg
      241 aacatatcgt ggtaatcgaa aaactaccgc gtactgcctc aggtaaaata caaaagtttt
      301 tgttaagaaa agatattatg cggcggttaa ctcaggatgt ctgtgaagag attgaataag
      361 tttcatcttc ggggatcaca taaccccggc gactgaacgc cgccggggat ttattttatt
      421 tcttcagttc agccaagctt aaccaggttt gcaccacggt gtccgggttc agagacaggc
      481 tatcgatccc ctcttccatc aaccatgcgg caaagtcttc gtggtcggac ggaccctgac
      541 cgcaaatccc gacatatttg ccctgtttct tcgcggcacg gatagccatc gacagcagtg
      601 ctttcaccgc atcgttgcgc tcatcgaaca attcagacac cacgccggag tcacggtcca
      661 gaccgagcgc cagctgcgtc atatcgtttg agccaattga gaagccgtcg aaatattcga
      721 ggaactgctc ggccagcaag gcgttggacg ggatttcaca catcatgatg attttcagcc
      781 cgttctcacc acgcttcagc ccctgacgcg ccagttcctc aaccaccgct ttcgcctgat
      841 ctacggtacg cacgaacggg atcatgatct caacgttggt cagccccatg tcgttgcgca
      901 cacgtttcac tgcttcgcac tccagcgcga agcagtcgcg gaagctgtcg gaaacatagc
      961 gtcccgcgcc acggaagccg agcatcgggt tctcttcatc tggctcgtaa cgctcaccac
     1021 cgaccaggtt ggcatattcg ttcgatttaa aatcagagag acggacaatg acgcgcttcg
     1081 gataaaacgc ggcacccagc gtcgcgatcc cttcagtcag acgaccaacg taaaattcac
     1141 gcggagaatc aaaacctttc atcatctcgc ggatttcatt ttgcaactgc ggttcctgat
     1201 cgtcaaactc aagcagtgcg cgtgggtgga cgccaatcat acggttgatg ataaattcca
     1261 gacgcgcaag gcccacgcct tcgttcggca ggcaggcgaa gtcgaaagca cggtccgggt
     1321 taccgacgtt catcatcact ttcaacggca gatccggcat catttctacg ctggagcttt
     1381 tcacgctaaa ttccagcaac tccgcataga cgtaaccggt atcaccttcg gcacaagaaa
     1441 cagtgacgtt ctcaccgtct ttcatccgtt ctgttgcatc accacagccc actaccgccg
     1501 gaatgcccag ttcacgagcg atgatcgccg cgtgacaggt acgaccgcca cggttggtga
     1561 cgatggcaga tgctttcttc atgatcggtt cccagtccgg gtcggtcatg tcagtgacca
     1621 gcacgtcacc aggttcgatg cggttcattt cgctgatctc atggatgact ttcaccggac
     1681 ccgcgccgat gcgatgaccg atagcacggc cttcggcgat aatcttaccc tgtgaatgca
     1741 gcgtataacg ctccatgacc tgaccgcgtg agcgcacggt ttccggacgc gcctgcacaa
     1801 tgaacagttt gccagtgtgg ccatctttcg cccactcaat atccatcggg cgaccgtagt
     1861 gtttctcaat ttgtacggcc tgttttgcca gttcctgcac ttcttcgttg gtcagcgaga
     1921 agatgtcacg ctgttcctgc ggtacgtctt cgattttaac ctgcttgccg tgctcctggg
     1981 tcggcgcgta aaccatgcgg atttttttcg accccatggt gcgacgcacg atagccgggc
     2041 gattcgccgc cagtgtcggt ttatggacgt aaaactcatc cgggttaacc gcaccctgca
     2101 cgaccatttc cccaaggccc catgcggaag tgataaacac cacctggtca aagccggatt
     2161 cggtatcaat ggagaacatc acgccagaag atgcgaggtc ggagcgcacc atccgctgaa
     2221 caccggcaga gagcgccacg ccacggtgat cgtaaccctg gtgcacacga taagagatgg
     2281 cgcgatcgtt aaacagagaa gcaaatacat gcttcactgc cacgagaacg gcgtcaaaac
     2341 cctgaacgtt gaggaaggtt tcctgctgac cggcaaaaga agcgtccggc atatcttctg
     2401 cggtggcgga ggagcgcacc gcaaaagagg cgttttcgtc atcggcggaa agctgtgcat
     2461 aggcttcgcg gatggcgttt tccagctcag gctggaaggg agtgtcgata atccactggc
     2521 ggatttgcgc gcccgctttc gcaagctgag taacatcgtc aatatccgtt ttatccagca
     2581 gttcataaat gcgctggttt acgccgcttt ggtccagaaa ctggttaaac gcgtcggcgg
     2641 ttgtggcgaa accattcgga acggaaacac ccattccgga aaggttagta atcatttcac
     2701 ccagggaggc atttttgccc ccaaccctgt ctacatcatt catgccgagt tggttatacc
     2761 aaagcaccag cggtgacgag ccattgttgg acatcgaaca atccttttgt gataaatgaa
     2821 cggtttgaga aacacatttc tgcgcattta tctttgcata tttaaccgga tgaaaaaaac
     2881 ggtgaatcgt tcaagcaaat atattttttt actttttaag actgatccca gcgttgcgca
     2941 aatctgctct cttcgacaat ttccacataa accatcggta taaacgaaac cataaaaatg
     3001 aaatgctgtt ttcataaaaa ataaaattga aggttcattt tataaaccag acataacgtc
     3061 ctacgcttct gtgcgttttt aatttatgct ttcatagaat tatgtctgca tcacgggaag
     3121 aacaaaatgg ataatgctgt tgatcgccac gttttttata tttctgatgg tacggcaata
     3181 actgcggagg tattaggaca cgcagtaatg tcacaatttc ccgtcactat cagcagcatt
     3241 acgctgccgt ttgtcgaaaa tgagagccgt gcacgggcag tgaaggatca gattgacgca
     3301 atttatcacc agacaggtgt gcgcccgctg gtcttctact ccatcgtgtt gccggagatt
     3361 cgcgccatca tcttgcaaag tgaaggcttt tgccaggata tcgttcaggc gctggttgcc
     3421 ccgctacaac aagagatgaa actggatcca acgccgattg ctcatcgtac ccatggcctt
     3481 aaccctaata atctcaataa atatgatgcg cgcattgcgg cgattgatta caccctcgcc
     3541 cacgatgacg gcatttcgtt gcgcaatctg gatcaggctc aggtgatcct gctcggtgtt
     3601 tctcgctgtg gtaaaacccc caccagtctt tatctggcaa tgcaatttgg tatccgcgcg
     3661 gcaaactacc cctttattgc cgacgatatg gataatctgg tgctacccgc gtcgctcaaa
     3721 ccgcttcagc ataaattgtt cggcctgact atcgacccgg aacgtctggc ggcgattcgc
     3781 gaggaacgtc gggagaacag tcgctatgcc tcgcttcgtc agtgcaggat ggaagtcgcg
     3841 gaagtggaag cgttgtaccg taaaaatcag atcccgtgga ttaacagtac caattattcg
     3901 gtagaagaga ttgccaccaa gatcctcgat atcatgggcc ttagtcgccg aatgtactag
     3961 agaactagtg cattagctta tttttttgtt atgatgctaa ccacccggcg aggtgtgaca
     4021 cacctcgcac ttgaaatcag cagcgattgg tttatcgtga tgcgcatcac ttcccggcag
     4081 tcctgccgta gaagcaacaa atttctgaga cttgtaatga acagaactga cgaactccgt
     4141 actgcgcgta ttgagagcct ggtaacgccc gccgaactcg cgctacggta tcccgtaacg
     4201 cctggcgccg ccacccatgt caccgactcc cgccgcagaa ttgaaaaaat actcaatggt
     4261 gaagataagc gactgttggt cattattggc ccctgctcga tccacgatct caccgctgca
     4321 atggagtacg ccccccgtct gcagtcgctg cgcaaccagt accagtcacg gctggaaatc
     4381 gtaatgcgca cctattttga aaaaccacga actgttgtcg gctggaaagg actaatctcc
     4441 gatccagatt taaacggcag ctatcgggta aatcacggtc tggagctggc gcgcaaatta
     4501 cttttacagg taaatgagct gggcgtccca accgcgactg agttcctcga tatggtgacc
     4561 ggtcagttta ttgctgattt aatcagttgg ggcgcgattg gcgcacgtac taccgaaagt
     4621 cagatccacc gtgaaatggc ttcggcactc tcctgtccgg taggttttaa aaatggtacc
     4681 gatggcaata cgcggattgc tgtggatgct atccgcgcag cccgcgccag ccatatgttc
     4741 ctctcgccag acaaaaatgg tcagatgacc atctatcaga ccagcggcaa cccgtatggc
     4801 cacattatta tgcgtggcgg caaaaaaccg aattatcatg ccgatgatat cgccgcagcc
     4861 tgcgatacgc tgcacgagtt tgatttacct gaacatctgg tggtggattt cagccacggt
     4921 aactgccaga agcagcaccg tcgccagtta gaagtttgtg aggatatttg tcagcaaatc
     4981 cgcaatggct ctacggcgat tgctggaatt atggcggaaa gtttcctgct cgaaggaacg
     5041 caaaaaatcg tcggcagtca gccgctcact tacggtcaat ccattaccga cccgtgtctg
     5101 ggctgggaag ataccgaacg cctggtcgaa aaactcgcct ctgcggtaga tacccgcttc
     5161 tgaatgcgtg cccattcctg acggaatggg catttctgcg caacttgttg tcttctcaac
     5221 aaattactgc ttgctctggc ctgccataat attgataata agaatcattg ttatatcaat
     5281 tattattaat ctttatgcgt tatacggata gcagaaaact cacgcctgaa acggacgcca
     5341 atcacaaggc cgcttccccg cagcccatcc ggcgaattac cagccagaca ctgttaggtc
     5401 cggatggcaa actgattatc gatcatgacg ggcaagaata tctgctccgt aaaactcagg
     5461 ctggcaagct gctgttaact aagtagcctt taactcgagc agctgacttc cagccgttta
     5521 ccccagtcag gtggacgact gacatagtca tcatctctat cgctgaaagg atttcgcaac
     5581 gccccatgca ggcggtgcaa ttccgtcata tcaccctttt ctgccgcttc aatcgcccgt
     5641 tgcgccaacc agttgcgcaa taccagagcg gggttaacgc tttgcatcaa ctgctgacgc
     5701 tcactatcgc taacctcgtc ttgttgcaaa cgcccccgat aacgggcaaa ccagccatca
     5761 aatgccgcac gatcaataaa ctcatcacgt agcggtgacg ccgtgctgtg ctgctcggtc
     5821 agactcagca tgcggaatgt gcgggtataa tcgctgcgct ctcgcgccat cagactgaat
     5881 aattcattca gtagcgcgtt atcctctttt tgctccgtga tgaagcccag tttctgccgc
     5941 atccgttgtc cataatgcgt caacaaaacc tgctgatagc tgtccagggc ctcattcagg
     6001 gcatctacgg caacaaatgg tgacaatgtc tgcgccagac gctgtaaatt ccacaacgcg
     6061 acagcaggtt gattatcaaa actataacgc ccttgatgat ccgagtgatt acaaataaaa
     6121 ccgggttcgt aatcatcaag aaaaccaaac ggcccgtaat caagcgtcag tcccagcagc
     6181 gacatgttgt cggtattcat caccccatgt gcaaagccga ccgtctgcca ttgggcaatt
     6241 aacgacgcgg tacgtgcgac aacatcgcta aaccagagac ggtatttgtc ctcatcatct
     6301 tcaagatgtg accagtaatg acgaatagca aagtcagcta actgacgaac cttttccgac
     6361 tcgcggcggt agtaaaaatg ttcgaaatga ccaaagcgca gatgacttgg tgccacacgc
     6421 atcagcatcg cgcctggctc cgccgtttcc cgatacactg gcgaatcgct ggtgacgata
     6481 cttaacgcgc gggtcgtcgg aatgcccaga taatgcatcg cctcactggc gagactttct
     6541 cgtatcgtcg aacgtaaaac cgcccgtcca tcacccattc gcgaataagg cgtcaggcca
     6601 gcacctttca gatgccagtc cattgtagtg ccatcagcaa gcagttgttc gccgagtaaa
     6661 atgccgcgcc catcacccag ttggcccgcc cagacgccga actgatgacc actgtaaacc
     6721 tgcgccagtg gtgacatacc aggcagtagg gcttcgccgc cccagacacc tgcgccattt
     6781 ttaaacagcg acgatggaat actcagcgtg ttagccagtt cggtattatg ccaaatcagc
     6841 cgggcattat ttaaaggcgt aggggaaagt gctgtatagg tttctggcaa ttcatcgcgc
     6901 cagcgggtaa caaaagacag ggtcatagat cctcctgttt gatagtgtag acggttactc
     6961 tcgtcttaaa caccagcaaa cagaagggtt atcgctgaac caacgtcgtt atctgattta
     7021 tgggaacggc aggccacagg catccctgga gagcatggaa atcgaagggg gtaatctgtg
     7081 ccaatatttc tgccgtatca atgcctcccg caatgatgca attgcaacaa ggggaaattt
     7141 gcgcctggat cgcacgaata aacggttcga aggagcggtg cgtgatttgt tgctgaataa
     7201 aacttttatc cagcatgacg cgggtgaaaa gaccatcaaa aaccgctttc attgtgctat
     7261 tgcctgctcc caaattacct aacacaaggg gatacacttg cgataaggat aaaagaccgc
     7321 gattatcttt tccttcattg agatgcgggt aattctcatt aatcaacagt tcaataaacg
     7381 gatattttaa tagttcgcct gcgtaattat ccctctctaa taataatgta gccacttgtg
     7441 gcgttaaatt aagccaggca aagagtttat gttgaataaa aaaatgttgg catgatttta
     7501 gtaattccaa ctgctctgaa aataattgcc aatgttgctc ctcggtaagt tgcgctataa
     7561 cccgactcgt cgggatacgc accgtgccat cttcactgga gaaatgggta attaactcaa
     7621 cgccgacaag gacttgctgg ttatctctta tcggcaggaa atagcaatca gaatgataaa
     7681 gattctccaa aaaaatcttc atggtaaccg tccctctcga aggatgtttt tcagtatccg
     7741 gctaaaaggg atgaggccat aagacataac aattacagaa ggagtaactt tcatttgttc
     7801 catgttaacc actttttcag gggtcctttt tttagattat cctgattata aacgaataat
     7861 ccatttacgg aatttttgtc tgcaaaatac tactgtattc aggagtaaat ggacggcgat
     7921 gtatcatcgc cgtccatttt tcgac
//
LOCUS       DADMKF010000173         7944 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.176, whole genome shotgun sequence.
ACCESSION   DADMKF010000173 DADMKF010000000
VERSION     DADMKF010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7944
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.176"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            111..443
                     /locus_tag="JNC24_003529"
     CDS             111..443
                     /locus_tag="JNC24_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HAY9160196.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            590..1951
                     /locus_tag="JNC24_003530"
     CDS             590..1951
                     /locus_tag="JNC24_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HAY9160197.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIRNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(2281..2598)
                     /locus_tag="JNC24_003531"
     CDS             complement(2281..2598)
                     /locus_tag="JNC24_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9160198.1"
                     /translation="MKKIAAISLISIFLISGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
     gene            3004..3903
                     /gene="yegS"
                     /locus_tag="JNC24_003532"
     CDS             3004..3903
                     /gene="yegS"
                     /locus_tag="JNC24_003532"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HAY9160199.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARYVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDGIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCLLLR"
     gene            complement(3985..4764)
                     /locus_tag="JNC24_003533"
     CDS             complement(3985..4764)
                     /locus_tag="JNC24_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAY9160200.1"
                     /translation="MTMNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRF
                     LEQKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMT
                     VILDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMH
                     GSIAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKF
                     NRRGFNQVLPIEKIDIIITDDAVSEVDKLALQKTRVKLITV"
     gene            complement(4864..5904)
                     /gene="gatD"
                     /locus_tag="JNC24_003534"
     CDS             complement(4864..5904)
                     /gene="gatD"
                     /locus_tag="JNC24_003534"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HAY9160201.1"
                     /translation="MKSMVNDTDGIVRVAESVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTYPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFTQAVRDIA
                     RNAMPGKVLLIP"
     gene            complement(5952..7307)
                     /locus_tag="JNC24_003535"
     CDS             complement(5952..7307)
                     /locus_tag="JNC24_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAY9160202.1"
                     /translation="MFSEVMRYILDLGPTVMLPIVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRVIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQLAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSTQNIPGFIIIGAIYLTGIFMTWRRARGFIKQEKTVLA
                     E"
     gene            complement(7311..7595)
                     /gene="gatB"
                     /locus_tag="JNC24_003536"
     CDS             complement(7311..7595)
                     /gene="gatB"
                     /locus_tag="JNC24_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HAY9160203.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQNHSIPVELIQCRVNEI
                     ETYMDGVHLICTTAKVDRSFGDIPLVHGMPFISGVGIEALQNKILTILQG"
     gene            complement(7626..>7944)
                     /gene="gatA"
                     /locus_tag="JNC24_003537"
     CDS             complement(7626..>7944)
                     /gene="gatA"
                     /locus_tag="JNC24_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HAY9160204.1"
                     /translation="EFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADD
                     DNDVEVSLVIALIVENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLD
                     SDE"
BASE COUNT         2148 a         2036 c         1783 g         1977 t
ORIGIN      
        1 tgacattatc tctccgccct ggcaggcatc agaaaatcgc tcatacttta atcggtaaac
       61 agcaccttta gatgctgttt tcgatacgca atttcaatca aggagtcatt atggctggtt
      121 ggtttgaact cagtaagagc agtgataatc agttccggtt tgtgctaaaa gcgggcaatg
      181 gtgagactat cctcaccagc gagctgtata catcaaaagc ctctgcggaa aagggcatcg
      241 cgtcggtgcg tagcaacagc ccgcaagaag aacgctatga gaaaaaaacg gcaagtaacg
      301 gcaaattcta tttcaatctg aaagccgcca atcatcaaat tatcggctcc agccagatgt
      361 acgccaccgc gcaatctcgt gaaaccggaa ttgcctccgt taaagccaat ggcacaagcc
      421 agacggtgaa agacaatacg taattacgat gccgggcgtg gggtgtcggc gtccggcgct
      481 ttaccagatc cccccacagc gctacaatgc ccgcccttaa agtgggggca ctcccctaac
      541 cgcttcatca ggtgaagcgg agctgacctg tcatcagaac gagagaatta tgtttaaacc
      601 ggaactcctt tccccggcgg gaacgctgaa aaatatgcgt tacgctttcg cttatggcgc
      661 agatgctgtt tatgcgggcc agccgcgtta ctccctgcgt gtgcgcaaca acgaattcaa
      721 ccacgaaaat cttcagctcg gcatcaatga agcccacgcg ctggggaaaa agttttatgt
      781 cgtggtcaac attgcaccgc acaacgccaa gctgaaaacc tttatccgtg acctgaaacc
      841 ggtggtggaa atggggccgg atgcgctgat tatgtccgat ccagggctga ttatgctggt
      901 gcgtgagcac ttccctgaaa tgccgatcca cctctcggtg caggctaacg ccgtaaactg
      961 ggcgacggtg aaattctggc agcaaatggg cctgacccgc gtgatcctct ctcgcgagct
     1021 gtcgctggaa gagattgaag agatccgcaa tcaggtgccg gatatggaga ttgagatctt
     1081 cgttcacggt gcgctgtgca tggcctactc cggtcgctgc ctgctctctg gctatatcaa
     1141 caagcgcgat ccgaaccagg gtacctgcac caacgcctgc cgctgggagt acaacgtcca
     1201 ggaagggaaa gaagatgacg ttggcaacat cgtacacaag tacgagccga ttccggtgca
     1261 aaatgttgag ccgacgctag gtatcggcgc gccaaccgac aaagtgttta tgatcgaaga
     1321 agcccagcgt ccgggcgagt atatgaccgc gtttgaagat gagcacggca cttacatcat
     1381 gaactcgaaa gatctgcgcg ccatcgccca tgtagaacgc ctgaccaaaa tgggcgtgca
     1441 ttcgctgaaa atcgaaggcc gtactaaatc tttctactat tgcgcacgca ccgcacaggt
     1501 ttatcgtaaa gctatcgatg atgccgctgc gggcaaaccg ttcgatacca gcctgctgga
     1561 aactctggaa ggtctggcgc atcgtggcta taccgaaggt ttcctgcgtc gtcatactca
     1621 cgacgattat cagaactacg aatacggtta ttcagtttct gaccgccagc agtttgttgg
     1681 tgagtttacc ggtgagcgca agggggacct cgcagcggta gcggtgaaaa ataaattctc
     1741 cgttggcgac agccttgagc tgatgacgcc gcaaggcaac attaacttta cccttgagca
     1801 catggaaaac gccaaaggtg aagcgatgcc ggtcgcacca ggcgatggtt atactgtgtg
     1861 gctcccggtc ccgcaggatc ttgagctaaa ttacgcgctg ctgatgcgta atttctccgg
     1921 ggaaaccacg cgtaacccac acggtaagtg attaatttcg attatttttc ccggatggaa
     1981 aattcttaga aaccgatcac atacagctgc atttattaag gttatcatcc gtttcgctga
     2041 aaaacataac ccataaaatg ctagctgtac caggaaccac ctccttagcc tgtgtaatct
     2101 cccttacacg ggcttatttt ttatgcataa gccctatccc tggtcaccgt cttccattga
     2161 ccacatcgat agaatctccc ttcatagcac gatgcctttc acgtaacggc atcgtgctcg
     2221 cacaggttcc ggctaagcac aaccagaacg cgcatgtttg atgcttacca aaaaatattc
     2281 tcactctcca catttgaatg tcagacgagc gacgccatgt aatcctgcac cttctgtctt
     2341 caggtcaact atctgcattt ttttgccctg agtaacacag aaatgggctg catcattttt
     2401 tactatattt tctgcaccag atattcttcc cctagctaaa gaagcttcgg cttcggtgta
     2461 gtattggtta tcgagtttac gctgaatatt acttttatat gcaagaccaa atttaccgat
     2521 acttgtttca tcattatgca cagcacaacc agatataaga aaaatactaa ttaatgatat
     2581 agcagctatc tttttcatct caccttcccc cattaaatac caacgacact ctctagtgtt
     2641 taaatataat aatggcatga ttattataat tgaataggat tataataaat gttctgtaca
     2701 acatttccta cataagtagg aattacggac attgaggccc ttcagggtaa ctccatgggg
     2761 gctttaatat attatattga agatgccact gtttagttga atattaggta tatgctcttt
     2821 tttgaaattt atcggtggca gccgttagta ttcgctgtcc ccattgcaag ctcctggtgg
     2881 taaccactga atcctccata cttgaactga ctttttatcc tccgactttc atcctgttct
     2941 gactccacct tttgttttct gctctacact atctacagac caatcataaa ggcacatacg
     3001 atcatggcag aatttcccgc cagcttactg attcttaatg gcaaaagtac tgacaatcta
     3061 cccttgcgcg aagcaattat gctgttgcgt gaggaaggaa tgacgatcca tgtgcgggtc
     3121 acctgggaga aaggcgatgc cgcacgatat gtagaggagg cccggaagtt gggcgtcgca
     3181 acggtgattg ccggtggtgg cgatggcacc attaatgaag tttctacggc gttgattcag
     3241 tgtgaggggg atggcatacc cgcgctggga attttgccat taggaaccgc caatgatttt
     3301 gccaccagtg tagggattcc tgaggcactg gataaggcgc tgaaactggc aattgccggt
     3361 aacgccattg cgatagatat ggcgcaggtc aacaaacaaa cctgttttat taatatggcg
     3421 acaggcggat ttgggacgcg tattaccaca gaaacgccgg aaaaattaaa agccgcgctg
     3481 ggtggcgtct cttacatcat tcatggctta atgcgcatgg atactctgca accggaccgt
     3541 tgtgaaatcc gcggtgaaaa ctttcactgg caaggtgacg ccctggtcat tggtattggt
     3601 aacgggcgtc aggccggtgg cggtcagcaa ttgtgtccga acgcgttaat taacgatggc
     3661 ttgctgcaac tgcgcatttt taccggcgat gaaattcttc cggctctcgt atcaacctta
     3721 aaatctgacg aagataaccc gaatattatc gaaggcgctt cgtcgtggtt tgatatacaa
     3781 gccccacacg aaatcacttt taatcttgat ggcgaaccgt tgagcggaca aaactttcat
     3841 attgaaatac ttccggcggc gttgcgttgt cgattaccac cagattgtct attattgcgt
     3901 taatcaaact attctctctt gaaccaggga gaatccgaaa tgccggatgc ggtgtaaata
     3961 ccttatccgg catacagaac aatactacac cgtaattaat tttacccgtg tcttctgcaa
     4021 tgccagttta tccacctctg atacagcatc atcggtaata ataatgtcaa ttttttcgat
     4081 tggcagtacc tgattaaaac cgcgacggtt aaatttcgat gaatcgagaa cggcaatcac
     4141 tttattagcg gcggtgacca tcgcaccgct aatcgaataa ccttcattaa aggtcgtaat
     4201 accattaacc gcatcaatac catcagcacc gacaaacatt aaatcggcat taatatcctg
     4261 caatgaacgc tcggcaatgg aaccatgcat cgagcgcgtt ttatggcgga ccgtgccacc
     4321 acagacgacc agagtaatgt ctttattttc ggaaagggca aacgctgccg ggagactgtt
     4381 ggtaatcacc gtgatatttt tagcggtcat taatccttcg gcgataagca ttgtcgtact
     4441 tccgctgtcg agaataacgg tcatcccttc atggatcatt ttgactgccg cctgcgctat
     4501 tctgtttttt ggcgcgctgg cgagctgaaa tcgctcttta aacccgactt cctgggtctc
     4561 ggtttcacta ttaccggaca ttattttcgc cgcaccgcca tgaaagcgcg taaccacgcc
     4621 tttttgttcg agaaagcgca aatcggcacg gattgtcgct tccgaggcag caaatactcc
     4681 cgccagatcc tgaacaagca cggttccctg ttcattcact aattggatga tcttattcct
     4741 tcgctcgaat gaattcatcg tcatttaccc atattcaatt gtggctagtg taaacgaagg
     4801 gggaccattg gtgaataatc tgttttgcac aataccgggc cagcatcacg ctggcccgcg
     4861 gtttcatgga atgagcaaca ctttgcccgg catggcatta cgagcgatgt cacgcaccgc
     4921 ctgggtgaag ctttcaaagc ttccacggtg agcgattaat ggctccaggc ttaacttacg
     4981 ttctgtcagc aaccggctcg ccgtttccca ctcctgcccc ggccaagggc tggaatagtt
     5041 catccaactg ccgataaccg tcagctcttt acgcaatatt ttgccaaacg ttgccgatgt
     5101 taaatgcaga tcctgatgca acgtgcccac cagcgccagt tgggcatgag gaccggcaat
     5161 ctctaccgcc agttcgacag tttgcggtac gccagccgtc tcgaggataa gctgattaaa
     5221 gcgcagttcg cgtaaaacgc tctgcatttg cggcgcgctc atttcgctac tgttaaatgt
     5281 ttgcatcgca ccgaaagatt ttgccaatgc cagtttttct gagctaatgt cgatcgccgt
     5341 cacactcttt gctcccagcg cgacagcgca ctgaatagcc agcaggccaa tggttccggc
     5401 accaataata ataacgtttt tattctcaca accttgcgct aaatgaaaag catgcaggcc
     5461 aacggtaatc ggctcaataa aagccccatc ctcaataggc atatccgtgg gtagagcaaa
     5521 gacatttttt cgcttaacga caatatattc agcaaatcca ccatcacgcc gcgagccaat
     5581 aaaatcatat tttgcgcact gggaataaaa ccctttcaaa cactctggat aagtaaaaca
     5641 gggtaataac ggcacacagg caaccgcatc gccagggtgt aaatcatcaa caccggatcc
     5701 caccgcatca atatagccgc taaattcatg gcctaacgtt attggataat aatgtgcacc
     5761 atttttaaat atcctgggta aatcggaacc acataagccc gagctggcaa tttttacccg
     5821 cacctcatcc tgatgtttaa tttcaggaat gacgctttct gcaacgcgca cgataccatc
     5881 agtatcattc accattgatt tcataaaaac tcctgattgt taagggggat aacctccccc
     5941 tcaggtaaaa attattctgc gagaacggtt ttctcttgtt taataaaacc acgcgctcta
     6001 cgccaggtca tgaaaatacc ggtaagataa attgcgccga taataatgaa accgggaata
     6061 ttttgcgtgg agaaaacctg aatcagtaac caggtaatgg gagaaccgcc ctgatccatt
     6121 gaagccacca tacccccggc ttttaacgcc ccagcattag ccgccagttg ggtgtgcaaa
     6181 ccaatagttt gcgtcgcgat ccacagggtg atgctcataa tgatgacacc cgagattaag
     6241 gtgcggaaca gatttccacg atgcaccgcg accgccatcg ccacgaagaa gccgatggtg
     6301 gcaagatcgc caaacggcag tacctgatta cccggaacac aaacagcaat taaaatggtg
     6361 agcgggataa aaatcaggct tgccgatacc accgccgtat gccccagcag taatgcggga
     6421 tccaggccaa tcaggaactc ctgaccgccg aacttcgcct gtaaacgact acgggcctgc
     6481 ttagcgatgg gcgttaaacc atccatgatg ggtttaatca cccgtggcat cagcagcata
     6541 actgccgccg tttttaccgc cagctgcaat acacctttga catcgtaacc ggcgaggatg
     6601 ccgataatca gccccatcac aaaacccacg gtgacaggct cgccaaatgg accaaatttg
     6661 cgctgaatat cgtcggcgct aaatttaatt cggttaacgc ctgggatttt ctcgatgata
     6721 gcatcgacca gcaccgcaat cggccccata tacgccgacg taccgtgcgg aatagcaata
     6781 ccttccagct caaagaaatt tcgggtatcg cgggcaaacc agtcgccgag cttataaaca
     6841 aacgccgcgt gaattaccac acctgccatc cctatcatcc atgaaccggt tgccagatgc
     6901 agcaacgcgc cggtgaaggt catatgccag atattccaga tatcaacatt taccacccgc
     6961 gtcatacggg tcagtaacat cgccacgtta accagaatcg caatcggaat cgccaccagc
     7021 gcaatttgcg acgcccaggt cattggtgaa gagcccggcc agccgacatc gaccacatgc
     7081 agattcaggt cgaaattttc cgccatcgct ttcgccgccg gaccaatgga atccagcatt
     7141 aagccaatca caaggccaat gccaacaaag ccaatcccga tatgcagacc cgctttaaag
     7201 caatcgcctg ccttcatgcc taatatttta gaaaaaataa tgatgacaat cggaagcatc
     7261 accgtagggc cgaggtcgag aatataacgc atgacttctg aaaacatagg tcacccctgt
     7321 aagatagtca gaattttatt ttgtaatgct tcgataccga caccagaaat aaaaggcatg
     7381 ccgtgaacta acggaatatc gccaaaacta cgatccactt tggcagtggt gcatatcaaa
     7441 tgcacaccat ccatataggt ttctatttca ttaacccgac actggattaa ttcaacagga
     7501 atactatgat tctgacacaa ctctttaatt tcttccgccg ccatcgtaga ggtcgcaacc
     7561 gcgcctccgc aagcgacaat aatcttgcgt ttcataaata gtccttttta ttgttacaga
     7621 gggatttatt cgtctgaatc taaaacatac tttgtgaagt attcctttaa ctgggtttca
     7681 ggaagagtga ttagtgtctc gacgatatcg ggctgttgta acttgccaaa taaacagcgt
     7741 aaaagtttca attgctgctg cggattctcc acaatcaacg caataaccaa cgatacctcc
     7801 acgtcgttat catcatccgc ttgctgaaaa tgaactttat ttgttggcct taacagataa
     7861 atggctgacg acttagcatg aatcgcctca caatgcggta ttgcaatggc gtgctgctca
     7921 agcattatcc cggtagggaa ttct
//
LOCUS       DADMKF010000174         7777 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.177, whole genome shotgun sequence.
ACCESSION   DADMKF010000174 DADMKF010000000
VERSION     DADMKF010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7777
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.177"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(1..1168)
                     /locus_tag="JNC24_003538"
                     /pseudo
     CDS             complement(1..1168)
                     /locus_tag="JNC24_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000532143.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecQ"
     gene            complement(1297..1780)
                     /locus_tag="JNC24_003539"
                     /pseudo
     CDS             complement(1297..1780)
                     /locus_tag="JNC24_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410808.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
     gene            complement(1921..3261)
                     /gene="gntU"
                     /locus_tag="JNC24_003540"
     CDS             complement(1921..3261)
                     /gene="gntU"
                     /locus_tag="JNC24_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HAY9160205.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILHETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTDTNLVKLIIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDINEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNNSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(3265..3792)
                     /gene="gntK"
                     /locus_tag="JNC24_003541"
     CDS             complement(3265..3792)
                     /gene="gntK"
                     /locus_tag="JNC24_003541"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HAY9160206.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDAAFAMQRTNKVSLIVCSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(3931..4926)
                     /gene="gntR"
                     /locus_tag="JNC24_003542"
     CDS             complement(3931..4926)
                     /gene="gntR"
                     /locus_tag="JNC24_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HAY9160207.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHCHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(5150..5845)
                     /locus_tag="JNC24_003543"
     CDS             complement(5150..5845)
                     /locus_tag="JNC24_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HAY9160208.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(5968..7005)
                     /locus_tag="JNC24_003544"
     CDS             complement(5968..7005)
                     /locus_tag="JNC24_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAY9160209.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYILNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRVLEAGKNVLVEKPFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDICSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAK"
     gene            7339..>7777
                     /gene="yhhY"
                     /locus_tag="JNC24_003545"
     CDS             7339..>7777
                     /gene="yhhY"
                     /locus_tag="JNC24_003545"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAY9160210.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQHPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKYGFEIEGTGKKYA"
BASE COUNT         1978 a         2101 c         1871 g         1827 t
ORIGIN      
        1 ggttttgctg cattttctgc ggtggattgt ccacgacttt ttcaatggca tcaataaaag
       61 gtaagccaag tttcgccgcc agtcgccggg caaaatcagg aaccaggtcg aggtgcttta
      121 atgaaggaac gcagcatacc caggttggtt caggatgcgg attccagcgt tggcgaacca
      181 tttccgcaca tgcctccacc agctcatcac tgaatcgccc cgtttttttc tcctgtgcta
      241 cctgctttcc ccacccggaa tctccccaac gagaaaggac tcgccccgta gatccttgta
      301 aacccgcagg caagttccct ttaaatccgt attgcgtaaa agctccagat gctacctgtt
      361 tattgagatt taatggcagg tcagcgtgtt taataaataa atttgccgca tggagtaagc
      421 cgctatcaat atcgggcgac aacaacggat gctgaagaca actgctacat ttaccacaac
      481 gttcggcact tggctcatcg agtgcgcggc gcagaaattg cattttgcac tccttggtcg
      541 tgatatagct ttcaacatct gccagctcat tttttctctg attcatcaaa tggttaattc
      601 gttcatggtc aggagagaaa ctgacgatag ttctgcgcca taatttctcg gtatacacca
      661 caggcgatgg attttcagct accaataatt tcagtgcttt ttctatttgc ccataacgga
      721 gattcgtccg ttgttcaatg cctcgtaggg taagaccgtc attctcgcta agtacgttga
      781 gtatttcatg aatttgcgcc tccgcaggaa aggcgctttc acggaagaat ttatgaatag
      841 cgcggtcttc accaccacaa agcaatatgc caactgcgga atctatagca cgcccggcac
      901 gccctacctg ttggtagtaa ccgacaatag accccggcat ttgataatga atgacgaaac
      961 ctaaatcagg tttatcaaac cccataccta atgccgtcgt cgcgaccagc actttgattt
     1021 tatttgccag cagcgcctgc tccagatatt ccctggcagt attagaatct tccgcgcctt
     1081 cacaagtcac gccgctgtag taagcaaatg cactgatacc atttgtcctt aaccactcgg
     1141 cgacaagttc ggcatcgcga gtagtcaggg tataaacaat tcccgatttg gaaaactgag
     1201 ggataaccgt tgctaaccat gccagacggg atgactgttc acctaagact aaggcatcaa
     1261 gcgccaaact ttcgcgagcc agcgttccac gctgaatcac aatgtcaccc aattgctgac
     1321 ggatatcctc aacgacacga ttattcgctg tcgcggttgt acccagaaca ggggtattcg
     1381 cagtttcaag aaaatcctga ttcgccaacc gttcaggtga aatcagcaga caatctacac
     1441 caccccgcag caacttatcg ctaatgcgct gccattcttc cctgtttgta gaattcaacg
     1501 tttcagccgt aatacctaac cgttcagcgg cggcaacctg gttacgcatc agggcaagca
     1561 aaggagagat gataattgtc gggccagcgc cgcgatcgcg gaagatttta ctggcgatga
     1621 agtacacggc acttttcccc cagccagtgc gttggacaac cagcagtttt cgccgctgat
     1681 taaccaccgc atctatagct tcccattgcc cttccctgaa agtagcgctg gtgttactta
     1741 acatccgctg taacagcaat tcagctccgt gtttctccat catcttctcc ctcgtccgtt
     1801 aacgcagtga ttagaacgga taatgttccg gtctttttgc gagagagatc aaaaagatga
     1861 agacaggaga taagagaaat gaattactgc gagcaagctc gcagtaattt gtcgcagtaa
     1921 ttaactcaac agctggaacg caatcatccc aacaatggca ccaacagtgc cgaggatggt
     1981 ttccatcatg gtccaggttt tcagcgtttc ggcttcggtc gcgccggtaa atttaccgaa
     2041 caaccagaaa ccagcgtcgt taacgtggct gacaacaatc gaaccaccgg cgatacaaat
     2101 cgacagcgcc gccatttgcg caccggaatt gttcagttgt tcaatgaccg gcatcaccag
     2161 ccccaccgcc gttaaacagg caacggtggc agacccctga atgatgcgca ctgcagctgc
     2221 cagcacgaag caggtgatag caatcggcag gcccatgccg gttaacgctt cgcccagtgc
     2281 cggacctacg ccagagtcaa ccagcacctg tttgaacacg ccgcctgcac caatcaccag
     2341 cagaatgatc cccgccggtt gcagcgcgtg accgcaaatc tccatcactt tgtcttttgg
     2401 catgccctga cgcattgcca ggccataaat cgctaccaga caagcaacca gaatcgcggt
     2461 aaacggatga ccaataaact cgaaccattc gtaagcggta gagccttctg gcacaaaacg
     2521 cgcggcaatg gttttcagcc ccaccagcac cagcggcaac aggatcagcg acaggctgaa
     2581 tccgaaagat ggcattttac cttcgccgag atgcggctcg ttgatgtcgt caggaatatg
     2641 cagctcaacg taacggctga tgaagttacc ccacagcggc ccggcaataa tcattcccgg
     2701 aattgccgca cacaggccaa tcaggatcat ccagccaaaa tcggcgttca tctgcgatgc
     2761 cagcagcatt ggcgctggcc ctggcaccag gaacgccgcc gctgccgcca cgcctgcgaa
     2821 taatgggatt atcagcttca ccaggttcgt atcggtgtgg cgcgccattg agaaagcaac
     2881 gctaatcagc agaacaatcg ccacttcaaa gaacagcggt agcgcacaga ccagccccgc
     2941 aaggccgatg gcataatgcg cgcggctgtg accgaaggat ttgagcattt tgacggcaat
     3001 ctgatcgact gcgccggttt catgtaagat cttgccaaac atagctccca gggcgacaac
     3061 caccgccagg aagccgaggg tgcctcccat ccctttttcc atcgtcgctg cgattttatc
     3121 gagcggcata ccggaaaaaa ggccagcccc catggacacc accatcaaag ccaggaaagc
     3181 gtgcatacgc gccttcatga ctaaaaacag cagcagtaaa acagacccta ctgctgttaa
     3241 aacaagcgtt aatgtagtca ctacttattt gcctttttta ataacctcaa tggtgcttgc
     3301 cacaacacct tccagcggtt gatcgatatc caccaccagt acatcggttt cgtccgcacc
     3361 cggctcctgc agcgtttcaa actgcgtcac caacatttgg gttttaaaga aatggccttt
     3421 gcgcgctttc aggcggcttt caatcacgtc aaaatcgcct ttcaggtaga tgaaagagag
     3481 attcggatta ccttcacgca gcaagtcgcg atagtgtttt ttcaatgcag aacagacgat
     3541 cagtgacact ttattagtgc gctgcatagc aaacgcggcg tcgttcagcg cctgcaacca
     3601 gggtttgcga tcgtcgtcat tcagcggttc gccagacgcc attttttcga tattgcgccg
     3661 tggatggagg aaatcgccat caagaaacgc ggcatgaagt tgatgcgcca cttcactggc
     3721 gaccgcagat ttgccgctgc ccgatacgcc catcaagacg taaatgtggt gatcatggtt
     3781 agtcgtgctc aaagtggtgc ccccacaata caagaattaa aaatgttacg ggtaactgtt
     3841 atcggtaaca ttgtccagcc ggacaatatc agaagcaata tccatccgtg ccttaagtgt
     3901 ataagtgtga gctacttcaa atttgtaggc ttaaatagat ccgcccggtg acaaggtgaa
     3961 acctaaatct aacattttcg gtgtcacaga ttcgccacga atacgcgcca gcaggcgttc
     4021 agcgccaata ctgcccatcc gctcacgcgg cgtcagcacg ctcgctaacc gcggctccat
     4081 cacctggcca atgtcatgac cgtggaaacc ggcaatcgcc atatcgtcag gaaccttcaa
     4141 tcccagacgc tgacattcaa acgccgcgcc gaccgccagg tcatcgttgg tacagaacac
     4201 gccatccagc tgcggatatt cccgccgcgc ctggcgaatc agttcaatac cggaagagta
     4261 agaagaagat tgctcaacca tcacgctata cggcaccaga cctgcatcca gcatcgcctg
     4321 ttcgtatccc ttctgtttga tgatagtacg ttcgtcgaga cgcgcgccga gataggcaat
     4381 gtggcaatgc ccgcgagcaa taatggcggt ggtcatctgg cgtgccgctt caaagttatc
     4441 aaaaccgacg gcgatatcga ggcacggcga ctggctgtcc atgagttcca ccaccgggat
     4501 ccccgccact tcaatcatct ttaaggtgcg cggcgtgtgg gtacgttcgg tgaggatcag
     4561 tccgtcgata ttccaggaga gcatggattc gagacgttct tgctccattt ccggtttata
     4621 accgtagtga gccagcatgg tctgataacc gtgcgcgtcg gtgacgcttt cgattccgcg
     4681 taatacttcc gcgaaaacct ggttggtgag agaaggtaac aggacgccaa tcgcccggct
     4741 ggtggcgtta gagaggatat cgggcgcacg attgggaata tagcccagtt catcaagagc
     4801 ggcggcaatc ttgccgcgta gagcgacgga aacctgctcc gggttgcgta aaaaacggct
     4861 gaccgtcatt ttggtcacgc ctacacggtc agccacatcc tgaagtacgg gtcttttctt
     4921 tttcatcgtc ctgaaggtac aaaagagata gattctctta gtttaacacg gacgcatcag
     4981 aaccttcccg gggaaaacag tgcttgttta caattattag atatacacta aatcaattgc
     5041 atcgcattgt gctaacgacg cggtaaaacc ccggaagcgt gcatcagcac gcgaccggag
     5101 cgagcgagga agcctgcaaa aaggcaggct tcaaagatgc cgttgagttt taaaccggcg
     5161 gcagatcgaa cagtaacact tcgctgtcgc tatccgcatg gatggagatt gcctgctcat
     5221 cccagattgc cagaccatcg ctggtcgagg ctttcacgcc gttaatggtg acattgcctt
     5281 tcaccacctg gatccagacg cggcgttcag cggcaatctg atgcaccgac tgctcatctt
     5341 tcagcaacgc ccagcggtac agttccatat cctgatgcac tttcaatgaa ccatctcgcg
     5401 catccggcga gagcaccagc tgtttgccct gtacggcatc gaagcgacgc tgttcataac
     5461 gcggcgtaat accgttttct tcaggcatga tccagatctg atacagatgc agacgctcgg
     5521 tgctgcttgg gttgtactct gagtgacgaa tacccgtacc cgcactcata atctggaact
     5581 cacccgccgg aacctgctct ttattgccca tgctgtcctg atgctcaaca gtaccttcca
     5641 gcacgtaggt caaaatttcc atatctttat gcgggtgagt gccgaagccc tgccctgctt
     5701 caatcacgtc gtcgttaatc acgcgcagcg cggagaagcc cataaagttc ggatcgtaat
     5761 agttggcaaa agagaaagta tgccaggagt ccagccagcc atgatttgca tgaccacgtt
     5821 catttgcttt gcgtaagtag atcatttgta ttcgccccat gaatgatttc gatgggctta
     5881 gtgtggaccc gatctgcctg ggatgataga gggtgaaaat tgacccctct gttcaaaaaa
     5941 attgaactat tcgaggggcc atcaaactta cttcgcgaga gtcactgtgg agggagaggc
     6001 ttgctcaaat ccgcgttcaa ggatttccag attggtaaga acttcagatt ccttgacgta
     6061 atttggcgca ccgtgggtga tggtttgata caacgcatca taaacgcgcc cgtaatcgcc
     6121 catctccggc ttcatctctt ctctgaccgt cacgccctcg tcattgacat actccagcac
     6181 accgaccgaa tcatccgctg cgaatcccgg ttcgcccggc ataatattag ccttcaggct
     6241 ggtttcctgc tggtcgatac cgtatttaat aaacgaacct ttcttaccgt gaacgataaa
     6301 tttcggataa tcgattttca ccagatggct ggttttgacg atggctttca ggtcgccata
     6361 aaacagttgc gcttcaaagg tgtcgtcagg attggcttta ttacgcaggc tgcagatgtc
     6421 ataagcgacg tgatccgggc gaccgaacag agaaataatc tggtccatcg tatgcacacc
     6481 aaggccatag aacgcgccat cctgcggcaa cccaggtttg gtttctgcca ccgggcggta
     6541 atagtcaaaa tggctttcca cttcaacaat ctctcccagc ttaccgcttt caatcgcttt
     6601 tttcgccgtc aggaagcagg aatcaaagcg acgattctga tatggcgtga cggtcagccc
     6661 tttgcttttc gccagtgcaa acagctcttt cgcctgcgca agtgtcggag tgaacggttt
     6721 ttcgaccagc acatttttcc cggcttccag cacgcgcttc gcgtactcga agtggctgtc
     6781 cgcatgggtg cagacaacaa ccaacttaac atcgggatcg tttagcactt cgtcgagatc
     6841 gctggtgaaa tggatatgag agtaaatggg agcctgttct tccggcttcg catggcgacg
     6901 aaaaatatgc gcgacatgcc agctatcctt gcggttaagt atatacggca gatggtaacg
     6961 ggtggtgctt ttgccgaagc caataaaggc gcagttgatg accatgattt cgtccttttt
     7021 aaggtggtta tttacacctt agcgcaaagc ggacgtggtt cctactggag tgcgcataac
     7081 gaacacaagc actcccgtgg ataaattgag aacgaaagat caaaaaaaaa gccagcaccc
     7141 ggctggctaa gtaatactgg aagcaatgtg agcaatgtcg tgctttcagg ttctccgcga
     7201 gggtcttcct gatcgcgaga caataataat cattctcatt cgcacttgtc caacactttt
     7261 tgcaaaaaaa atgcatttga ctcgcatttg aaagtcaatg atgttgaaag ggacatttac
     7321 cccaaagagg acaaaggaat gagtgagata gtaatacgcc acgcagaaac acgggattac
     7381 gaggccatca ggcagattca cgcccagccg gaggtgtatt gcaacacact acaggtgcct
     7441 catccttccg atcatatgtg gcaggagcga ctcgccgatc gtcccggcat caagcaactc
     7501 gtcgcctgta ttgatggaga cgtcgtgggc catctcacca ttgacgtgca acagcaccca
     7561 cgccgcagtc atgttgccga ttttggtatc tgtgtcgact ctcgttggaa gaaccgcggc
     7621 gtcgccagcg ccctgatgcg agagatgatt gaaatgtgcg acaactggtt gcgggtagat
     7681 cgcattgaac taaccgtgtt tgtcgataac gccccggcaa ttaaggtcta taaaaaatac
     7741 ggctttgaaa ttgaagggac tggtaagaag tacgcat
//
LOCUS       DADMKF010000175         7746 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.178, whole genome shotgun sequence.
ACCESSION   DADMKF010000175 DADMKF010000000
VERSION     DADMKF010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7746
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.178"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            22..519
                     /locus_tag="JNC24_003546"
     CDS             22..519
                     /locus_tag="JNC24_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HAY9160211.1"
                     /translation="MSPSLRKAVAVAIGGGAVAIASVLITGPGGNDGLEGVSYILYKD
                     IIGVWTVCHGHTGKDIMPGKTYTEAECKALLNKDLATVARQINPYIKVDIPETTRGAL
                     YSFVYNVGAGNFRTSTLLRKINQGYIKGACDQLCRWTYAGGKQWKGLMTRREIEREVC
                     LWGQQ"
     gene            516..986
                     /locus_tag="JNC24_003547"
     CDS             516..986
                     /locus_tag="JNC24_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005013949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HAY9160212.1"
                     /translation="MSRVTAIISALVICIIVRLSWAVNHYRDNAITYKEQRDKNAREL
                     KLANATITDMQQRQRDADALDAKYTKELADAKAENDALRRKLDNGGRVLVKGKCPVPS
                     SAETSSASGMGNDATVELSPVAGRNVLGIRDGIISDQTALRMLQEYIRIQCLGG"
     gene            complement(1075..1587)
                     /locus_tag="JNC24_003548"
     CDS             complement(1075..1587)
                     /locus_tag="JNC24_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HAY9160213.1"
                     /translation="MMEWINALMNYFVLHPYRLWGLLFIIAFTKSTLLISAILPPASI
                     MMATVITVSKTTLSPWEAGITVMSGAWCGTIVNYHLGIIIGHIPQLACIISSRSNTIE
                     RVRLQLQNNSVSILFTSRFIAVLRYIAPLVAGMLQLHPVKVYTVSLISAASWSALYVG
                     SFSFVLPFFS"
     gene            1782..2546
                     /locus_tag="JNC24_003549"
     CDS             1782..2546
                     /locus_tag="JNC24_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9160214.1"
                     /translation="MAKPDWEAIESAYRAGVLSLRDIGEKYGVTEGAIRKRAKKLGWA
                     RSGGTQVCKNGTQKRKVRTSRKPAITGLTQKSTQLKTESTPDTKPIRGMRTDPPTNPF
                     QPGNQQALKHGGYARRLLLKDEVIEDAKALTLEDELFRLRANNLVAAENIGRWLVSLE
                     DANGDQERKMLIENISAAEKAMMRNTVRIESIVGTLATVGKIFADTAYRKAATDKVSL
                     EADRLRRDAGIDDGNGERDLNDFYSDIQTDAESGFT"
     gene            2497..3900
                     /locus_tag="JNC24_003550"
     CDS             2497..3900
                     /locus_tag="JNC24_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HAY9160215.1"
                     /translation="MTSTLTSKPTLNPALRSFWTMRARNKVLYGGRSSSKSWDAAGIA
                     IFLSNKYTLRFCCARQIQNKIEESVYTLLKIQIDRFGLRHRFRILNNKIINRVTGSEF
                     VFYGLWRNIEEIKSLEGIDVLWLEEAHALTEYQWKILEPTIRKEGSECWFIFNPGLVT
                     DFVWRNFVVDPPEGTLIRKINYDENPFLSDTMLKVIDAARRRDPDGFKHVYEGVPESD
                     DDAAIIKLSWIEAAVDAHKTLNFEPSGRKRIGFDVADSGTDKCANVYRHGSVVFWADE
                     WKAKEDELLKSCQRTYQAALEREADIVYDSIGVGASAGAKFSEINADRKSENAYARRV
                     NYQRFNAGAGVHEPDDEYNGIPNKDFFANLKAQAWWLVADRFRNTFNAINNGEQYPVD
                     ELISIDSRCPLLEKLKLELTTPHRDFDRNGRVMVESKKDLAKREIPSPNVADAFIMAF
                     APIDTSLDIWEQLGRQA"
     gene            3900..5366
                     /locus_tag="JNC24_003551"
                     /pseudo
     CDS             3900..5366
                     /locus_tag="JNC24_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706598.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1073 domain-containing protein"
     gene            5257..6000
                     /locus_tag="JNC24_003552"
     CDS             5257..6000
                     /locus_tag="JNC24_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HAY9160216.1"
                     /translation="MTNRRSRQAEIRYRTSLRRIARAVGDIVNGRYDGSNDSITEIIA
                     ALERYSEIITPWATKVAENFTADLTRQNEKVWRQHSKNISRELRNLVESAPVGQVMQS
                     IIAEQVKYIKSLPLEAADRVYDIQNKAIEAVVTGGRAEQFAKEIASTGDVAKSRADLI
                     ARTELGRATGALDMARAIAIGSDGYIWRTADDGDVRDSHDHMKGKFVRWDSPPTLDGM
                     TGHAGELPNCRCYKEIVFVRVPFAMKRAA"
     gene            6004..7224
                     /locus_tag="JNC24_003553"
     CDS             6004..7224
                     /locus_tag="JNC24_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2213 domain-containing protein"
                     /protein_id="HAY9160217.1"
                     /translation="MKYFFETRLGETRYRLADGSLLCKDVPIGRTGKQLYGADDLPKL
                     KPDKFGEIVVTRSPEQVFHPATLASFEGMSITILHPEDENGNVRLVNPENWKELAVGH
                     LQNVRRGTGEQSDLMLADLIVKDESAIQLIEDGLREVSCGYDAEYEQTEPGKAEQVDI
                     TGNHVALVPKGRAGNRCAIGDRDTMANQKKNWWNRMRAAIKTGDADTMNELVESAPAS
                     VTGDEGDLPQGVNLNINLSPQQPLPDKAPEMGGDPTGDSDDDLKTLLKALLAKLERNA
                     TGDNDNKPDDNPTGDGEDDEEETTITGDSAWRAEVIVPGIDLSRKMKPTAFKREVLAS
                     ADKTLVRQIVGDADIRKLPKQSVDMAFNAVSEVAKGRNTRATTGDAQRLNMGMTSIAS
                     LNKQNAEFWANRKG"
     gene            7228..7734
                     /locus_tag="JNC24_003554"
     CDS             7228..7734
                     /locus_tag="JNC24_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9160218.1"
                     /translation="MNNVFLYRMPVGIAGAVSRPQDLTVEPVVLKSDNAFAAYGLAGK
                     YDDDGFFVPLADGDTADKVKGIYVRPYPTTSQPDMVRQVGTGKNFPGDAMKRGYVTVN
                     LGSDFDASTIKKGDPVYVVVSTDESIKVPLGGFMSTSVSGKNVVLTNAEFTGAGDADG
                     NAEISWKI"
BASE COUNT         2063 a         1845 c         2111 g         1727 t
ORIGIN      
        1 taaggctgcg agaggtgaat aatgtcgcca tcattacgca aggctgttgc agttgctatt
       61 ggtggcgggg ctgttgctat agcatctgtg ttaatcactg gcccaggtgg taacgatggt
      121 ctggaaggtg tcagctacat actatacaaa gatatcattg gtgtatggac tgtatgtcac
      181 ggacacaccg gaaaagacat catgcctggt aaaacgtata ccgaagcaga atgcaaagct
      241 ctcctgaata aagaccttgc cactgtcgcc agacaaatta acccgtacat caaagtcgat
      301 ataccggaaa caacgcgcgg cgctctttac tcgttcgttt acaacgtggg cgctggcaat
      361 ttcagaacat cgacgcttct tcgcaaaata aaccagggct atatcaaagg cgcatgtgac
      421 cagctatgtc gctggacata tgctggcggt aagcaatgga aagggctgat gacccgtcgt
      481 gagattgagc gtgaagtctg tttgtggggg cagcaatgag cagagtcacc gcgattatct
      541 ccgctctggt tatctgcatc atcgtccgcc tgtcatgggc tgttaatcat taccgtgata
      601 acgccattac ctacaaagag cagcgcgaca aaaacgcaag agaactgaag ctggcgaacg
      661 caaccattac tgacatgcag cagcgccagc gtgatgctga tgcactcgat gctaaataca
      721 cgaaggagtt agctgatgcg aaagctgaaa atgatgctct tcggcgcaag cttgataatg
      781 gtggtcgggt gctcgtcaaa ggaaaatgcc ctgtgccatc ctcagccgaa acctccagcg
      841 cctccggcat gggcaatgat gccaccgtcg aactctctcc agttgctgga cgaaacgttc
      901 tcggtatccg ggacggaatc atcagcgacc agacagcatt gagaatgctt caggagtaca
      961 tcaggatcca atgccttggg gggtagcggt aattttactc attatccttc aaatcagatt
     1021 ctgttgtcag aggaatgggg gaagctggat tcgcaagagc tttttatggg cagtttagct
     1081 aaaaaaaggc aacacgaagc taaaactacc aacataaagt gctgaccagc tagcagctga
     1141 aatcagactc actgtataca ctttcactgg atgaagttgc aacattcctg ccaccaaagg
     1201 agcaatataa cgcagcacgg cgataaagcg cgaagtgaaa agtattgaca cagagttatt
     1261 ttgcaactga aggcgaaccc gttctattgt attggaacgg cttgatatta tacatgcaag
     1321 ttgagggata tgcccaatta ttataccaag atggtaatta acgatagtac cgcaccatgc
     1381 gccactcatg accgtgattc cagcttccca tggtgaaaga gtcgtcttgc tgactgtaat
     1441 tacagtggcc atcataattg atgctggtgg caatatcgcc gaaatgagta aagtggattt
     1501 tgtgaacgct attataaaaa gaagccccca taggcgatat ggatgaagta caaaatagtt
     1561 catcaaagca tttatccact ccatcaatgt ctttccttct gtgaattttt ctttgctatt
     1621 taaccgtgat acgacttaat ttaggcttaa acaccttaca tttacaaggt actcctgtgg
     1681 ggggccctgc cacggagcgt cgcaggagtg agtgatgagg aagaattaaa gcaacctatt
     1741 gtgacaggca gcgataacag taaatcacag gaagcataat catggcaaaa ccggactggg
     1801 aggccatcga atcggcatac cgggccggag tccttagcct ccgtgatata ggcgagaaat
     1861 acggcgttac agaaggggct atcaggaaga gggccaaaaa gcttggttgg gcacgcagtg
     1921 gcggtacgca ggtttgcaaa aatggtacgc aaaaaaggaa agtgcgtacc agcagaaagc
     1981 ctgccattac tggccttaca caaaaaagta cgcaactaaa aacagaatct acaccggata
     2041 cgaaaccgat acgcggaatg cgtaccgatc ccccgactaa cccattccaa cccggtaacc
     2101 agcaggcatt aaaacacggt ggttatgccc gtcgccttct gctcaaagat gaggtgatag
     2161 aggacgctaa agcgttgacg ctcgaggacg aattatttcg ccttcgtgct aacaaccttg
     2221 tcgctgcaga gaatattggt cggtggttgg tgtcgctgga agatgctaat ggggaccagg
     2281 aaaggaagat gctgattgaa aatatcagcg ccgccgagaa agcaatgatg cgcaatacag
     2341 ttcgtattga gtccatcgtt ggcacgcttg cgacggtagg aaaaatattt gctgatacag
     2401 cctaccgcaa ggccgccact gataaggtgt ctctggaggc tgatcgtctt cgccgtgatg
     2461 caggtattga tgatggcaat ggagagcgtg acctcaatga cttctactct gacatccaaa
     2521 ccgacgctga atccggcttt acgtagtttc tggactatgc gggcacgtaa caaagtgctt
     2581 tatggtggtc ggtcatcgtc aaaatcatgg gatgccgctg gcattgccat atttctgtcg
     2641 aataaataca ccctgcgttt ttgttgtgcc cgtcagatcc agaataaaat cgaagagtcg
     2701 gtgtataccc tgctcaaaat tcagatagac aggtttggtc tgcggcaccg tttccgtatt
     2761 ctgaacaaca aaatcattaa ccgggttact ggctcggaat ttgtttttta tggattatgg
     2821 cgcaacatcg aagaaattaa gtcactggag gggatcgatg tgttgtggct ggaagaagcc
     2881 cacgcactga cggaatacca gtggaaaatt ctggagccaa cgatccgtaa agagggttcg
     2941 gaatgctggt tcatattcaa ccccggactt gttactgatt tcgtctggcg caacttcgtt
     3001 gttgatccgc ccgaaggcac tctcatccgc aaaattaact atgacgaaaa tccgtttctg
     3061 tctgacacca tgcttaaggt tatcgacgcg gcgcgacgcc gtgatccgga tggttttaaa
     3121 catgtgtatg agggcgttcc ggagtctgat gatgatgcgg caatcatcaa actgtcttgg
     3181 atagaagccg cagtggatgc gcacaaaacg ttaaatttcg aacccagtgg aagaaagcgt
     3241 attggctttg acgtggctga cagtggtaca gataagtgcg ctaacgttta ccgtcacgga
     3301 tccgttgttt tctgggccga cgaatggaag gccaaagaag atgaattact gaagagctgc
     3361 cagcgtactt atcaggcggc gctggagcgt gaagcagata ttgtttacga ctctatcggt
     3421 gttggtgcgt ctgccggtgc taaattctct gaaattaacg ctgaccggaa gagcgagaac
     3481 gcatacgcgc gacgtgtgaa ttaccagagg tttaacgccg gtgctggtgt gcatgagcca
     3541 gatgacgagt acaacggcat ccccaacaaa gactttttcg caaatcttaa ggctcaggca
     3601 tggtggctgg tggctgaccg tttcagaaat acgtttaacg ccattaacaa cggagaacag
     3661 tatcctgtgg atgagctgat cagcatagat tctcgttgtc cgttgcttga aaagctgaaa
     3721 ctggaactga caacacctca tcgtgatttc gaccgtaacg gacgtgtgat ggtcgaaagt
     3781 aaaaaagacc tcgcaaaacg cgagataccg tcaccaaacg ttgctgatgc attcattatg
     3841 gccttcgcgc caatcgatac atcgctggat atctgggaac agctggggag acaggcctga
     3901 tggcacgaaa caaacaagcc ctgcggcgaa ctgtgcaggc cacagctgat ggttatgaga
     3961 attttattgc ccgcgtaggg atgcagacac ctaaccagca ctcagcatcc acctaccggg
     4021 ctaatttcac cagtcgtaac cgcatgctgg tggaatggtc ctatcgttca tcctagatca
     4081 tcggcgaagc agtcgatgct atcccggatg atatgacccg caaaggcatt cgcatcactt
     4141 cggaaattga tgcaaaagat cgtggcattc tcgaatcaca actggatgag ttgcaaatct
     4201 gggatgcgct gaatgacgtg ctgaaatggt cgcgcctcta cggcggcgcg gtgggtttca
     4261 tcatgattga ggggtaggca ccaatgaccc cgctgcgacc cgaaaccatc ggtaagggca
     4321 agtttaaggg gattctcccg ctcgaccgct ggatggtcga cccggcactg acccgccgca
     4381 ttaaagatat ggggccggac ctgggtaaac ctgagtttta cgatgtggtg accacagcaa
     4441 cgggaattcc tgcctggcgc attcatcaca gtcgcctgat tcgctttgat ggcgtcacgt
     4501 tgccatttca gcagaagatg accgagaacg aatggggaat gtcggttgta gagcgtatct
     4561 gggatcgtct taccgcgttc gacagcgcta ctgtcggcgc ggcgcagctg gtctacaagg
     4621 cgcatttgcg tacctacagc gtggagaagc tacgcgagct tatcgcactt ggtggtcctg
     4681 cgtatgaagc gttgctgaag aatatcgacc tgattcgaca gttccagagt aatgaaggca
     4741 tgacgctcat ggactcgcgg gataagtttg aaacccatca gtacagcttc agtggtctgg
     4801 atgacatcct ttcgcagttt gtagaacaga ttagtggcgc tgttggtatc ccactggtgc
     4861 ggttgttcgg acagtccccg aaaggatttt ctaccggtga tgcagacctt gccaactatt
     4921 acgatcgcat cagttcgttg caggagaggc gtttacgtct tccggtgcgg cggatactgg
     4981 acatcatgca tcgttcggaa cttggcaagc cgctgccgga cgatttcacg tttgagttta
     5041 acccgctctg gcaaatgtct gatgtcgatc gctcaacggt ggcgttaaac accaccaacg
     5101 caatcagtac ggcgctgggt gatggtctga tgacactgaa agccgctatg actgatttgc
     5161 gagaaaattc tgacgtaacc ggcatcgggg catccattac cgacgaggac atcgagaatg
     5221 ccgaagatga agcgccgccc ggcatcggcg aacctgatga cgaaccgcag gagccgtcag
     5281 gcggaaatcc ggtatcgaac cagcctacgc aggatagcga gggcggtcgg agacatcgta
     5341 aatggtcgct acgatggttc aaatgacagt atcacggaaa ttattgcggc gctggagcgt
     5401 tacagtgaaa tcatcacccc ctgggcgaca aaggtcgcgg aaaactttac tgcggaccta
     5461 acccggcaga acgagaaagt ttggcggcaa cacagcaaga acatcagtcg cgagctccgc
     5521 aatcttgtgg aaagcgctcc tgtgggccag gtgatgcaat ccatcatcgc cgaacaggtc
     5581 aagtacatca aatctctgcc tctcgaggcc gcagacaggg tgtacgacat ccagaacaaa
     5641 gcgatagagg ctgttgtcac cggtggtcgg gcggagcagt ttgctaaaga gattgcatct
     5701 accggcgatg ttgctaagtc tagggccgat ctgattgccc gaacggaact gggaagagca
     5761 acgggcgcgc tggatatggc ccgagcgata gctattggct cggatggtta tatttggcgt
     5821 acagccgatg atggcgatgt ccgagattcc cacgatcaca tgaaaggtaa attcgtccgc
     5881 tgggattcac ctccaacttt ggacggcatg accggccacg cgggcgagct gccaaactgc
     5941 cgctgctata aagagatcgt gtttgttcgc gttccattcg caatgaaaag ggcagcataa
     6001 cccatgaaat acttttttga gaccaggctc ggggaaaccc gataccgcct ggctgacggc
     6061 tcgttgctgt gcaaagacgt gccgatagga cgaacaggta agcagctcta tggtgctgat
     6121 gacctgccaa aactgaaacc cgataagttc ggtgaaatag tcgtcacgcg ttctcctgag
     6181 caggtattcc atccggccac gcttgcctca ttcgaaggga tgagcatcac gatcctgcat
     6241 cctgaagatg aaaacgggaa tgtgcggctg gtaaatcccg agaactggaa agagcttgct
     6301 gtcgggcacc tccagaatgt ccggcgcggg acgggtgagc agtctgattt gatgctggct
     6361 gaccttatcg tcaaagacga aagcgccatt cagcttatcg aagatggcct gcgcgaagtg
     6421 tcgtgcggct atgacgcgga gtacgagcag accgagccag gtaaagccga gcaggtcgat
     6481 attaccggaa accatgtggc tcttgtccct aaaggcagag ccggaaatcg ttgtgcaatt
     6541 ggagacagag acacaatggc aaatcaaaag aaaaactggt ggaaccgcat gcgtgcggcc
     6601 atcaagacag gagatgccga caccatgaac gaactggtgg agtcggctcc cgcatcggtt
     6661 acaggagatg agggggattt gccgcagggc gttaatctca acatcaacct gtccccgcag
     6721 caaccactac cggacaaagc accagagatg ggcggagatc caaccggcga cagtgatgat
     6781 gacctcaaaa cattactgaa agccctgctg gctaagctgg aaagaaatgc cacgggcgat
     6841 aacgataata agcctgacga taatccgacc ggtgacggcg aggacgatga agaggaaacc
     6901 acgattactg gtgactcagc ctggcgtgcc gaagttatcg ttccgggtat cgatctgagc
     6961 cgtaagatga aaccgaccgc gttcaaacgc gaggttctgg cttctgctga caaaacgctg
     7021 gttcgccaga tagtcggtga tgcggatatc cgcaaattac cgaaacaatc ggtcgacatg
     7081 gcgtttaatg ccgtgtctga ggttgccaaa gggcgaaaca cccgcgccac caccggcgat
     7141 gcacagcgcc taaacatggg catgaccagt atcgcttccc tgaacaaaca aaacgctgaa
     7201 ttctgggcaa accgtaaagg gtaaaaaatg aataatgtat ttctgtaccg gatgcctgtt
     7261 ggcattgccg gggctgtctc tcgcccgcag gacttaaccg tcgaaccggt ggtccttaaa
     7321 tccgataacg ccttcgctgc ctatggcctg gctggtaaat acgatgatga cggttttttc
     7381 gtgccgctgg cagatggtga taccgcagac aaggtgaagg ggatctacgt gcgcccttat
     7441 ccgaccacgt cgcagccgga catggttcgc caggtgggaa caggcaagaa cttcccgggc
     7501 gacgccatga agcgtggcta cgtgaccgtt aatctcggtt ctgattttga tgccagcact
     7561 atcaaaaaag gcgatccggt atacgttgtc gtctccactg atgaatccat caaagtgccg
     7621 ctgggtggat tcatgtccac gtcagtcagt ggcaaaaacg tggtgctgac caacgctgaa
     7681 ttcacaggtg ccggtgatgc tgacggcaat gcagaaattt cctggaagat ttaaggaaca
     7741 gacgaa
//
LOCUS       DADMKF010000176         7715 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.179, whole genome shotgun sequence.
ACCESSION   DADMKF010000176 DADMKF010000000
VERSION     DADMKF010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7715
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.179"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            <1..643
                     /locus_tag="JNC24_003555"
     CDS             <1..643
                     /locus_tag="JNC24_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAY9160219.1"
                     /translation="HAFITESDADGHVLTVREDLIWPLLEVLYPGTRETFGLPGICLS
                     LADKPDELAALEHYWQLIERESVEQLPGREHTLTLLAQAVFTLLLRNAKLDDHAASGM
                     RGELKLFQRFNMLIESHFHQHWTVPDYANELHITESRLTDICRRFANRPPKRLIFDRQ
                     LREAKRLLLFSDNAVNNIAWQLGFKDPAYFARFFNRLVGCSPSAYRAKKVPVT"
     gene            894..2456
                     /gene="hpaB"
                     /locus_tag="JNC24_003556"
     CDS             894..2456
                     /gene="hpaB"
                     /locus_tag="JNC24_003556"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
                     /protein_id="HAY9160220.1"
                     /translation="MKPEDFRASTQRPFTGEEYLKSLQDGREIYIYGERVKDVTTHPA
                     FRNAAASVAQLYDALHKPEMQDSLCWNTDTGSGGYTHKFFRVAKSADDLRQQRDAIAE
                     WSRLSYGWMGRTPDYKAAFGCALGANPGFYGQFEQNARNWYTRIQETGLYFNHAIVNP
                     PIDRHLPTDKVKDVYIKLEKETDAGIIVSGAKVVATNSALTHYNMIGFGSAQVMGENP
                     DFALMFVAPMDANGVKLISRASYEMVAGATGSPYDYPLSSRFDENDAILVMDNVLIPW
                     ENVLIYRDFDRCRRWTMEGGFARMYPLQACVRLAVKLDFITALLKKSLECTGTLEFRG
                     VQADLGEVVAWRNTFWALSDSMCSEATPWVNGAYLPDHATLQTYRVLAPMAYAKIKNI
                     IERNVTSGLIYLPSSARDLNNPQIDQYLAKYVRGSNGMDHVQRIKILKLMWDAIGSEF
                     GGRHELYEINYSGSQDEIRLQCLRQAQSSGNMDKMMAMVDRCLSEYDQNGWTVPHLHN
                     NDDINMLDKLLK"
     gene            2474..2986
                     /locus_tag="JNC24_003557"
     CDS             2474..2986
                     /locus_tag="JNC24_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HAY9160221.1"
                     /translation="MQLDEQRLRFRDAMASLSAAVNIITTEGDAGQCGITATAVCSVT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            3379..5529
                     /gene="btsT"
                     /locus_tag="JNC24_003558"
     CDS             3379..5529
                     /gene="btsT"
                     /locus_tag="JNC24_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HAY9160222.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGHLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            5579..5782
                     /locus_tag="JNC24_003559"
     CDS             5579..5782
                     /locus_tag="JNC24_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HAY9160223.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            5793..6749
                     /gene="yjiA"
                     /locus_tag="JNC24_003560"
     CDS             5793..6749
                     /gene="yjiA"
                     /locus_tag="JNC24_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HAY9160224.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYEIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQRYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEDEIRAAFAGLKK"
     gene            complement(6827..>7715)
                     /locus_tag="JNC24_003561"
     CDS             complement(6827..>7715)
                     /locus_tag="JNC24_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin acylase"
                     /protein_id="HAY9160225.1"
                     /translation="RVTEIDRLLEQKPRLTADQAWDVIRQTSRQDLNLRLFLPTLQAA
                     TSGLTQSDPRRQLVDTLTRWDGINLLNDDGKTWQQPGSAILNVWLTSMLKRTVVAAVP
                     MPFDKWYSASGYETTQDGPTGSLNISVGAKILYEAVQGDKSPIPQAVDLFAGKPQQEV
                     VLAALEDTWETLSKRYGNNVSNWKTPAMALTFRANNFFGVPQAAAEETRHQAEYQNRG
                     TENDMIVFSPTTSDRPVLAWDVVAPGQSGFIAPDGTVDQHYEDQLKMYENFGRKSLWL
                     TKQDVEAHKESQEVLHVQR"
BASE COUNT         1784 a         2101 c         2021 g         1809 t
ORIGIN      
        1 tcatgcgttt attacggagt ctgacgccga cggtcatgtg ttgacggtac gggaagatct
       61 gatctggccc ctgctggaag ttctttatcc aggcactcgg gaaaccttcg gcctgccggg
      121 gatttgcctg tcactggcag ataaacccga cgaactggcg gcgctggaac actattggca
      181 actgatagag cgggaatcgg tggaacaact gcctggacgg gaacacaccc tgacgctact
      241 ggcacaggca gtgttcaccc tactgctgcg taatgcaaaa ctcgacgacc atgccgccag
      301 cgggatgcgc ggagaattaa aactgttcca gcgttttaat atgctgattg aaagccattt
      361 tcatcagcac tggacagtac cggattacgc taacgaactg catatcaccg aatcacgcct
      421 cacggacatc tgccgccgct ttgccaaccg tccgccaaaa cggttgattt ttgacaggca
      481 gctgcgagaa gccaagcggc tgctgctgtt ttctgataac gccgtgaaca atattgcctg
      541 gcaactcggt tttaaggatc cagcttattt tgcgcgcttt tttaatcgct tagtcggttg
      601 ctcgcccagt gcttatcgtg ccaaaaaagt acctgtgacg tgaaaatccc ttagccttaa
      661 cgggaaacca ggcaccacct gctattcccc tttctccgcc tggagcaagg ggatttagta
      721 acgtcaaaaa tcgctaaaag cgaccgcgat cacaaaaccg tacccgacct gaaaagtacc
      781 agccattgtc ccaaaagtgt cttcccgtaa tcgcccctgc aagcttcaat tgaataacaa
      841 aaaaaacaca caaaattaac aatcgtattc cgattaatac tgtagaggtc gacatgaaac
      901 cagaagattt ccgcgccagt acccaacgtc cgttcaccgg ggaagagtat ctgaaaagcc
      961 tgcaggatgg tcgcgagatc tatatctatg gcgagcgagt gaaagacgtc actactcatc
     1021 cggcatttcg taatgcggca gcgtctgttg cccaactgta cgacgcgctg cacaaaccgg
     1081 agatgcagga ctctctgtgc tggaacaccg acaccggcag cggcggctat acccataaat
     1141 tcttccgcgt ggcgaaaagt gccgacgacc tgcgccagca acgtgacgcc atcgctgagt
     1201 ggtcacgcct gagctatggc tggatgggcc gtaccccaga ctacaaagct gctttcggtt
     1261 gcgcactggg cgcgaatccg ggcttttacg gtcagttcga gcagaacgcc cgtaactggt
     1321 acacccgtat tcaggaaact ggcctctact ttaaccacgc gattgttaac ccaccgatcg
     1381 atcgtcattt gccgaccgat aaagtgaaag acgtttacat caagctggaa aaagagactg
     1441 acgccgggat tatcgttagc ggtgcgaaag tggttgccac caactcggcg ctgactcact
     1501 acaacatgat tggcttcggc tcggcacaag taatgggcga aaacccggac ttcgcactga
     1561 tgttcgttgc gccaatggat gccaatggcg tcaaattaat ctcccgcgcc tcttatgaga
     1621 tggtcgcggg tgctaccggc tcgccgtacg actacccgct ctccagccgc ttcgatgaga
     1681 acgatgcgat tctggtgatg gataacgtgc tgatcccatg ggaaaacgta ctgatctacc
     1741 gcgatttcga tcgctgccgt cgctggacaa tggaaggcgg ttttgcccgt atgtatccgc
     1801 tgcaagcctg tgtgcgcctg gcagtgaaac tcgacttcat tacagcactg ctgaaaaaat
     1861 cactcgaatg taccggcacc ctggagttcc gtggtgtaca ggccgatctc ggtgaagtgg
     1921 tggcgtggcg caacaccttc tgggcattga gtgactcgat gtgttctgaa gcgacgccgt
     1981 gggtcaacgg ggcttattta ccggatcatg ccacactgca aacctatcgc gtactggcac
     2041 caatggccta cgcgaagatc aaaaacatta tcgaacgcaa cgttaccagt ggcctgatct
     2101 atctcccttc cagtgcccgt gacctgaaca atccgcagat cgaccagtat ctggcgaagt
     2161 atgtgcgcgg ttcgaatggt atggatcacg tccagcgcat caagatcctc aaactgatgt
     2221 gggacgccat tggcagcgag tttggtggtc gccacgaact gtatgaaatc aactactccg
     2281 gtagccagga tgagattcgc ctacagtgtc tgcgccaggc acaaagctcc ggcaatatgg
     2341 acaagatgat ggcgatggtt gatcgctgcc tgtcggaata cgaccagaac ggctggactg
     2401 tgccgcactt gcacaacaac gacgatatca acatgctgga taagctgctg aaataacgca
     2461 gcaggaggtt aagatgcaat tagatgaaca gcgcctgcgc tttcgtgacg cgatggccag
     2521 cctgtcggca gcggtaaata ttatcaccac cgagggcgac gccggacaat gcgggattac
     2581 ggcaacggcc gtctgctcgg tcacggatac accaccatcg ctgatggtgt gcattaacgc
     2641 caacagtgcg atgaacccgg tttttcaggg caacggtaag ttgtgcgtca acgtcctcaa
     2701 ccatgagcag gaactgatgg cacgccactt cgcgggcatg acaggcatgg cgatggaaga
     2761 gcgttttagc ctctcatgct ggcaaaaagg tccgctggcg cagccggtgc tgaaaggttc
     2821 gctggccagt cttgaaggtg agatccgcga tgtgcaggca attggcacac atctggtgta
     2881 tctggtggag attaaaaaca tcatcctcag tgcagaaggt cacggactta tctactttaa
     2941 acgccgtttc catccggtga tgctggaaat ggaagctgcg atttaagctc cagccccttc
     3001 acgcattcgt cgaaggggca tttttattgt gatctcaatc acatatccac ccgatgaata
     3061 attcttgccc aaccgctcac cgccattcct gaccgcttac gtccctaaaa taaccactca
     3121 gttatttacc ttactttacg cgcgcgtaac tctggcaaca tcactgcagg atagcggtca
     3181 atttacctcc tcaaacgcaa cgcaaaccta gaacggcttc ggcctatgat taacaaatta
     3241 ctctatgaat tttggtttgc aggaaggcag caagtgagtg aatccccggg agcttacaac
     3301 agtaagtgac aggggtgaac gaacgcaact gccgcacctg taagccaaaa gacgacgagt
     3361 aaaatgccag gttttactat ggatacgaaa aaactattca agcacatacc ctgggtgatt
     3421 ctcggaatca tcggtgcatt ctgcctcgcg gtagttgcat tacgtcgggg ggagcacgtc
     3481 agcgccctgt ggatcgtggt cgcctctgtg tcggtgtatc tggtggcgta tcgctactac
     3541 agtctgtaca tcgcccagaa ggtgatgaaa ctcgacccca cgcgcgcgac gcctgcggtt
     3601 attaacaacg acggtctgaa ctacgttccg accaaccgtt acgtgttgtt tggtcaccac
     3661 ttcgccgcta tcgccggtgc tggtccgctg gtcggtccgg ttctcgccgc acagatgggc
     3721 cacctgcctg gcacgctgtg gctgctggcg ggggtagtac tggccggtgc ggttcaggac
     3781 tttatggtgc tgtttatctc ctctcgccgt aacggcgcat ctctcggtga gatgataaaa
     3841 gaagagatgg gaccagtacc ggggactatc gcgctgtttg gctgtttctt aatcatgatc
     3901 atcatccttg ccgtcctggc gctgattgtg gttaaagccc tggccgaaag tccgtggggt
     3961 gtcttcaccg tttgctcaac cgtaccgatt gcgctgttta tgggtatcta catgcgcttt
     4021 atccgtccgg ggcgtgtggg tgaagtctct gtcattggta tcgtgctgct ggttgcctct
     4081 atctacttcg gtggcgtgat tgctcacgat ccgtactggg gtccggcact gacctttaaa
     4141 gacaccacca ttaccttcgc gctgattggc tatgcgtttg tttccgcatt gctgccggtg
     4201 tggctgatcc tcgcaccgcg cgactatctg gcaaccttcc tgaaaatcgg cgttatcgtc
     4261 ggcctggcgc tgggtatcgt ggtgctgaac ccggaactga aaatgcctgc gatgacccag
     4321 tacattgacg gtactggccc gctgtggaaa ggtgctctgt tcccgttctt gttcattacc
     4381 atcgcctgtg gtgcggtatc tggcttccac gcactgatct cttccggtac gacgccgaaa
     4441 ctgctggcta atgaaaccga cgcgcgtttc attggctacg gcgcaatgct gatggagtcc
     4501 ttcgtggcga ttatggcgct ggttgctgcg tccatcatcg aaccgggtct ttacttcgcg
     4561 atgaacaccc cgcctgctgg ccttggcatc accatgccta acctgcatga aatgggtggc
     4621 gagaacgcgc cgatcatcat ggcgcagctg aaagacgtta ccgcacacgc ggcagcgacc
     4681 gtcagctctt ggggcttcgt gatttcgcca gagcagatcc tgcaaaccgc gaaagacatt
     4741 ggtgagcctt ctgtcctgaa ccgtgcaggt ggtgcgccaa cgctggcggt aggtatcgct
     4801 cacgtgttcc acaaagtgct gccgatggct gacatgggct tctggtatca cttcggtatt
     4861 ctgttcgaag ccctgttcat cctgaccgcg ctggatgcgg gtacccgttc tggccgtttt
     4921 atgctgcaag acctgctggg taacttcatc ccgttcctga aaaaaaccga ctctctggtt
     4981 gccggtatca tcggtactgc gggctgtgtg ggtctgtggg gctacctgct gtatcagggc
     5041 gtggtcgatc cgctgggcgg cgttaagagc ctgtggccgc tgttcggtat ctccaaccag
     5101 atgctggcag ccgtagcgct ggtactgggc accgttgtgc tgattaagat gaagcgcacc
     5161 caatacatct gggtaactgt tgttccggct gtatggctgc ttatctgcac cacctgggcg
     5221 ctgggtctga aactgttcag caccaacccg cagatggaag gcttcttcta catggcaagc
     5281 cagtacaaag agaagattgc taacggtact gacctgacgg cgcagcagat tgccaacatg
     5341 aaccacatcg ttgtgaacaa ctacaccaac gcaggcctga gtattctgtt cctgattgtg
     5401 gtgtacagca tcatcttcta cggtttcaaa acctggcttg cggtgcgtaa cagcgacaaa
     5461 cgtactgaca aagaaactcc gtacgttcca atcccggaag gcggcgtgaa gatctcttcg
     5521 caccactaac cgtgtttagc cccgcttcgg cggggctttg ttcttatcag agtgaactat
     5581 gtttggtaac ttaggacagg caaaaaaata tctcggccag gcggcgaaga tgttgattgg
     5641 cattccagac tacgacaact acgtcgagca tatgaagacc aaccatcccg acaagccgta
     5701 catgagctat gaagaattct tccgcgaacg ccagaacgcg cgctacggcg gcgatggtaa
     5761 aggcggtatg cgctgttgtt aatggagaga gcatgaaccc gattgcagtt accctactca
     5821 ccggtttttt aggcgcgggc aaaaccaccc tgctgcgcca tattcttaac gaacaacacg
     5881 gctacgagat tgccgtgatt gaaaacgaat tcggcgaagt ctccgttgat gatcaactga
     5941 ttggcgaccg cgccacgcag atcaaaacgc tgaccaacgg ctgcatctgc tgttcgcgct
     6001 ccaacgagct ggaagacgca ctactcgacc tgctggataa tctcgacaag ggcaatattc
     6061 agttcgaccg tctggtcatt gaatgcaccg gcatggccga tcccggcccg attattcaga
     6121 ccttcttctc ccatgagatt ttatgccagc gctacctgct ggacggggtg attgcgctgg
     6181 tggatgcggt acatgccgat gagcagatga accagttcac catcgcccag tcccaggttg
     6241 gctacgccga ccgtattctg ctgacgaaaa ccgacgtcgc aggcgaagca gagaaactgc
     6301 gtgaacgcct ggcgcgcatc aacgcccgcg caccggtcta caccgttacc cacggcgata
     6361 tcgacctggg ccttctgttc aacaccaacg gttttatgct ggaagaaaac gtcgtcagca
     6421 ccaaaccgcg tttccacttt atcgcggaca aacaaaacga tatttcgtcg attgtggtgg
     6481 aactggatta cccggtagat atcagcgaag tttcccgggt gatggaaaac ctgctgctgg
     6541 aatcggcgga taaactgctg cgttacaaag ggatgctgtg gattgacggc gaacctaacc
     6601 gcctgctgtt ccagggcgtc cagcgcctct acagcgccga ctgggaccgc ccgtggggcg
     6661 atgaaaaacc gcatagcacg atggtgttta tcgggattca gttgccggag gatgagattc
     6721 gggcggcgtt tgcgggactc aaaaagtaac cagccctcca acatcacaat acctgagact
     6781 ttatcaccct catattcctc cagaatatga gggcattcgg gcttaattat ctctgaacgt
     6841 gcaacacttc ctgcgactcc ttatgcgcct ccacatcctg cttcgttaac cagagcgact
     6901 tacgaccaaa attttcgtac attttcagct gatcttcata gtgctgatca actgttccat
     6961 cgggagcaat aaacccactc tgaccgggtg cgaccacatc ccaggcaagc accggacgat
     7021 cgcttgtcgt tggtgagaaa acaatcatat cgttttctgt tccacggttt tgatactccg
     7081 cctgatgacg cgtttcttcc gctgcggcct gcggtacacc aaagaaatta tttgcacgga
     7141 acgttaaggc cattgcaggt gttttccagt tactcacatt attgccatag cgtttggaaa
     7201 gagtctccca ggtatcttcc agcgcagcca acacaacctc ctgctgtggt ttcccagcaa
     7261 acagatcaac cgcctgtggg attggtgatt tgtctccctg caccgcctca tacaaaattt
     7321 ttgctccaac acttatattc agcgaaccag ttgggccgtc ctgggttgtt tcgtagccac
     7381 tggcgctgta ccacttatca aatggcatag gtacggcagc cactacggta cgcttcaaca
     7441 tactggtcag ccaaacgttc aggatggcag agcctggctg ctgccaggtt ttaccatcat
     7501 cattaagcaa attgatgcca tcccaacgtg ttaatgtatc caccaactga cggcgcggat
     7561 cgctctgtgt caaaccagat gtcgctgctt gcagagtagg taaaaaaagc ctcaggttaa
     7621 gatcctgacg actggtttgg cgaataacat cccatgcctg atcagcagtt aagcgtggct
     7681 tttgctcaag cagtcggtcg atctccgtaa cgcga
//
LOCUS       DADMKF010000177         7612 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.180, whole genome shotgun sequence.
ACCESSION   DADMKF010000177 DADMKF010000000
VERSION     DADMKF010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7612
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.180"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            101..1390
                     /locus_tag="JNC24_003562"
     CDS             101..1390
                     /locus_tag="JNC24_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HAY9160226.1"
                     /translation="MKGTIFAVALNHRSQLDAWQEAFQQSPYKAPPKTAVWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKVREEDAAEYIAGYALANDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPGDAILLGTPQARVEIQPGDRVRVLAEGFPPLENPVVDEREVTTHKSFPTQ
                     PHPHGTLFALGLNYADHASELEFKPPEEPLVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARNVSEADAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPML
                     STIVPKEAIPDPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGRLVNRIVSEDTAK"
     gene            1387..2853
                     /gene="hpaE"
                     /locus_tag="JNC24_003563"
     CDS             1387..2853
                     /gene="hpaE"
                     /locus_tag="JNC24_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HAY9160227.1"
                     /translation="MKKVNHWINGKNVAGNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWANLPMKERARLMRRLGDLIDQNVPEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALVSPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTADSLGELALEADIPAGVLNVVQGYGATAGDALVRHHDVR
                     AVSFTGGTATGRNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVGDPNDPNTQVGALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKPSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLRQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            2855..3706
                     /gene="hpaD"
                     /locus_tag="JNC24_003564"
     CDS             2855..3706
                     /gene="hpaD"
                     /locus_tag="JNC24_003564"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HAY9160228.1"
                     /translation="MGKLALAAKITHVPSMYHSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGVYTSNELPHFIRDMTYNYEGNPELGQLI
                     ADEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            3716..4096
                     /locus_tag="JNC24_003565"
     CDS             3716..4096
                     /locus_tag="JNC24_003565"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HAY9160229.1"
                     /translation="MPHFIVKCSDNIREEADLPGLFAKVNPTLAATGIFPLAGIRSRV
                     HWVDTWQMADGQRDYAFVHMTLKIGAGRSLESRQQAGEMLFELIKTHFAALMESRLLA
                     LSFEIEELHPTLNFKQNNVHALFK"
     gene            4207..5010
                     /gene="hpaH"
                     /locus_tag="JNC24_003566"
     CDS             4207..5010
                     /gene="hpaH"
                     /locus_tag="JNC24_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hepta-3-ene-1,7-dioic acid hydratase"
                     /protein_id="HAY9160230.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAEGRTLKGHKIGLTSKAMQASSQISEPDYGALLDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLEAGQIILGGSFTRPVPARKGDTFHVDYGNMGSISCRFV"
     gene            5021..5809
                     /gene="hpaI"
                     /locus_tag="JNC24_003567"
     CDS             5021..5809
                     /gene="hpaI"
                     /locus_tag="JNC24_003567"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HAY9160231.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGAGFDWLLIDGE
                     HTPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     EAVRATRYPPAGIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIREAGKAPGILIANEQL
                     AKRYLELGALFVAVGVDTTLLARAAEALAARFGAQATAVKPGVY"
     gene            5984..7360
                     /gene="hpaX"
                     /locus_tag="JNC24_003568"
     CDS             5984..7360
                     /gene="hpaX"
                     /locus_tag="JNC24_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001285781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HAY9160232.1"
                     /translation="MSDTSPAIPESIDPANQHKALTAGQQAVIKKLFRRLSVFLFVLF
                     IFSFLDRINIGFAGLTMGRDLGLSATMFGLATTLFYAAYVIFGIPSNIMLSIVGARRW
                     IATIMVLWGIASTATMFATGPTSLYVLRILVGITEAGFLPGILLYLTFWFPAYFRARA
                     NALFMVAMPVTTALGSIVSGYILSLDGVMALKGWQWLFLLEGFPSVLLGVMVWFWLDD
                     SPDKAKWLTKEDKKCLQEMMDNDRLTLVQPEGAISHHAMQQRSMWREIFTPVVMMYTL
                     AYFCLTNTLSAISIWTPQILQSFNQGSSNITIGMLAAVPQICTILGMVYWSRHSDRRQ
                     ERRHHTALPYLFAAAGWLLASATDHNMIQMLGIIMASTGSFSAMAIFWTTPDQSISLR
                     ARAIGIAVINATGNIGSALSPFMIGWLKDLTGSFNSGLWFVAALLVIGAGIIWAIPMQ
                     SSRPRATL"
     gene            7370..>7612
                     /locus_tag="JNC24_003569"
     CDS             7370..>7612
                     /locus_tag="JNC24_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003924737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9160233.1"
                     /translation="MCDRQIANIDISKEYDESLGTDDVHYQSFARMAAFFGRHMLPHR
                     HEQYFQMHFLNSGQIELQLDDHRYSVEAPLFVLTPPS"
BASE COUNT         1751 a         2135 c         2152 g         1574 t
ORIGIN      
        1 atcacattta catcacttac ttttgccaaa ctacgcaatc gggaataaaa gcaatcatta
       61 acatattaat gattaagatg atcgaatccg aggagtggta atgaaaggca ctatcttcgc
      121 cgtagcgttg aaccatcgca gccaacttga tgcatggcag gaagcgttcc agcaatcccc
      181 ctacaaagcc ccgcctaaaa ctgcggtctg gtttattaaa ccgcgcaata cggtgattgg
      241 ttgcggtgaa ccgattccct ttccacaggg tgaaaaggta ctgagcggtg cgactgttgc
      301 gctgattgtg ggaaaaacgg cgacgaaagt acgtgaagaa gatgcggcag agtacatcgc
      361 cggatatgcg ctggctaacg acgtcagcct gccggaagag agcttttacc gcccggcaat
      421 caaagcaaaa tgtcgtgatg gattctgccc cattggcgaa accgtggctc tcagcaatgt
      481 cgataatctg accatctata ccgagatcaa cgggcgtcct gccgatcact ggaataccgc
      541 cgatttacaa cgtaacgccg cgcagttgct gagcgccctg agcgaatttg ccacgctgaa
      601 tccaggcgac gccattctgc tcggcacgcc acaggcgcgc gtggaaatac agccaggcga
      661 tcgcgttcgt gttctcgcag aaggtttccc gccgctggaa aatccggtag tggacgaacg
      721 tgaagtgacc acgcacaaga gcttcccaac gcagccacac ccgcacggca cgctgtttgc
      781 cctcggcctg aactacgccg accacgccag cgaactggaa tttaagccac cggaagaacc
      841 gctggtgttc ctgaaagcgc caaataccct cactggcgat aaccagacct ccgtgcgtcc
      901 aaacaatatt gaatacatgc actatgaagc ggagctggtg gtagttattg gcaaacaggc
      961 gcgtaacgtc agcgaagccg atgccatgga ttatgtcgcg ggctacaccg tgtgtaacga
     1021 ctacgccatt cgtgactatc tggaaaacta ctaccgccct aacctgcggg taaaaagccg
     1081 cgacggactg acgccgatgc tttcaaccat cgtgccgaaa gaggcgatcc cggacccgca
     1141 taatctgacc cttcgcacct tcgtcaacgg cgagttacgc cagcaaggca ccaccgccga
     1201 tctgatcttc agcgtgccct tcctgatcgc ctacttaagt gaatttatga ccctgaatcc
     1261 gggcgacatg atcgccaccg gcacacccaa aggcttatct gacgtggtgc ctggcgatga
     1321 agtagtggtg gaagtagaag gcgtgggccg cctggtgaac cgaattgtga gtgaggatac
     1381 agcgaaatga aaaaagtaaa tcattggatc aacggcaaaa atgttgcagg taacgactac
     1441 ttccagacca ccaatccggc aacgggtgaa gtgctggcgg atgtggcctc tggcggtgaa
     1501 gcggagatca atcaggcggt agcggcagcg aaagaggcgt tcccgaaatg ggccaatctg
     1561 ccgatgaaag agcgtgcgcg cctgatgcgc cgtctgggcg atctgatcga ccagaacgtg
     1621 ccagagatcg ccgcgatgga aaccgcggac accggcctgc cgatccatca gaccaaaaat
     1681 gtgttgatcc cacgcgcttc ccacaacttt gaatttttcg cggaagtctg ccagcagatg
     1741 aacggcaaga cctatccggt tgacgacaag atgctcaact acacgctggt gcagccggtg
     1801 ggcgtttgtg cgctggtatc gccgtggaac gtaccgttta tgaccgccac atggaaggtc
     1861 gcgccgtgtc tggcgctggg caataccgcg gtactgaaaa tgtcggaact ctccccgctg
     1921 accgctgaca gcctgggtga gctggcgctg gaagccgata ttccggcagg cgtgctgaac
     1981 gtggtacagg gctacggcgc aaccgcaggg gatgcgctgg ttcgtcatca tgacgtacgt
     2041 gccgtgtcgt tcaccggcgg tacggccacc gggcgcaaca tcatgaaaaa cgccgggctg
     2101 aaaaaatact ccatggaact gggcggtaaa tcgccggtgc tgatttttga agatgccgat
     2161 attgaacgcg cgctggacgc cgccctgttc accatcttct cgatcaacgg cgaacgctgc
     2221 accgccggtt cgcgcatctt tattcagcaa agcatctacc cggaattcgt taaacgcttt
     2281 gccgaacgcg ccaaccgtct gcgcgtgggc gatccgaacg atccgaatac ccaggttggc
     2341 gcgcttatta gccagcagca ctgggaaaaa gtctccggct atatccgtct cggcattgaa
     2401 gaaggcgcaa ccctgctggc gggcggcccg gataaaccgt ccgacctgcc tgcgcacctg
     2461 aaaggcggca acttcctgcg cccaaccgtg ctggcagacg ttgataaccg tatgcgagtc
     2521 gcccaggaag agattttcgg gccggtcgcc tgcctgctgc cgtttaaaga cgaagccgaa
     2581 ggcttacgtc tggcaaacga cgtggagtac ggcctcgcgt cgtacatctg gacacaggat
     2641 gtcagcaaag tgttacgcct ggcgcgtggc attgaagctg gcatggtgtt cgtcaacacc
     2701 cagaacgtgc gtgacctgcg ccagccattt ggcggcgtaa aagcctccgg caccgggcgt
     2761 gaaggcggtg agtacagctt cgaagtgttc gcggaaatga agaacgtctg catttccatg
     2821 ggcgaccatc caattccgaa atggggagtc tgatatgggt aagttagcgt tagccgccaa
     2881 gatcacgcac gtaccgtcga tgtatcactc tgaactgcca gggaaaaacc acggttgccg
     2941 ccagggcgcg atcgacgggc ataaagagat cagcaagcgt tgccgggaaa tgggcgtcga
     3001 taccattatc gttttcgata cccactggct ggtcaacagt gcttatcaca tcaactgtgc
     3061 agaccatttt gaaggcgtct acaccagcaa cgagctgccg cattttatcc gcgatatgac
     3121 ctacaactac gagggcaacc cggagttggg gcagcttatt gccgatgaag ccttaaagct
     3181 cggcgtgcgg gcaaaagcgc acaacattcc cagcctgaaa ctggaatacg gcacgctggt
     3241 gccgatgcgc tacatgaatg aagacaagca cttcaaagtg gtctccattt cggctttctg
     3301 cacggttcac gattttgccg acagccgcaa gctgggcgaa gcgattctga aagcgatcga
     3361 acagtacgac ggcaccgtgg cggtccttgc cagcggttcg ttatcgcacc gctttattga
     3421 cgatcagcgt gcggaagaag ggatgaacag ctacacccgc gagttcgacc gccagatgga
     3481 cgagcgcgtg gtgaagttgt ggcgcgaagg ccagttcaaa gagttctgca atatgctgcc
     3541 ggagtacgcc gactactgct acggcgaagg caatatgcac gacacggtga tgctgctggg
     3601 gatgctcggc tgggataaat acgacggcaa ggtggagttt atcaccgagc tattcccaag
     3661 ctctggcacc ggtcaggtta acgctgtttt cccgcttccc gcgtaaggac gttttatgcc
     3721 gcactttatc gttaaatgca gtgacaacat ccgcgaagaa gccgacctgc cggggttgtt
     3781 cgccaaagtg aatccgacgc tggcagccac gggtattttt ccgctggcgg gtattcgcag
     3841 ccgcgtgcat tgggtcgata cctggcagat ggccgacggg cagcgtgatt atgccttcgt
     3901 gcatatgacg ttgaaaatcg gcgcaggtcg cagcctggaa agccgccagc aggcgggtga
     3961 aatgctgttt gaactgatta aaacgcactt cgccgccctg atggagagcc gcctgctggc
     4021 gttgtcgttt gagattgaag agctgcatcc gacgctgaat tttaaacaaa acaacgtgca
     4081 cgcattgttt aagtagcgcg cagattgccc ggtggcgctg cgcttaccgg gcctacaaaa
     4141 ccccaaaccg tacaccgtag gccggataag gcgcagccgc atccggcaat gccacaggat
     4201 atcgctatgt tcgataaaca cacccacacc ctgatcgccc agcgtctgga tcaggcagaa
     4261 aaacagcgcg aacagatccg cgcgatctcg ctggattacc cggagatcac catcgaagac
     4321 gcttacgcgg tgcagcgtga atgggttcgc ctgaaaatcg ccgaaggtcg cacgctgaaa
     4381 ggccacaaaa tcggcctgac ctcgaaagcg atgcaggcaa gctcgcagat cagcgaaccg
     4441 gattacggcg cgctgctgga tgacatgttc ttccacgatg gcagcgatat cccgaccgat
     4501 cgctttatcg tgccgcgcat tgaagtggag ctggctttcg tgctggcaaa accgctgcgt
     4561 gggccaaact gcacgctgtt cgacgtctac aacgccacgg actacgtgat cccggcgctg
     4621 gagctgatcg acgctcgctg ccacaacatc gatccggaaa cccagcgccc gcgcaaagtg
     4681 ttcgacacca tttctgataa cgccgccaac gccggggtga tcctcggcgg tcgtccgatc
     4741 aaaccagacg aactggatct gcgttggatc tccgccctga tgtatcgcaa tggcgtgatt
     4801 gaagaaaccg gcgtcgccgc tggcgtgctg aatcatccgg caaacggcgt ggcctggctg
     4861 gcgaacaaac tcgcaccgta tgacgtacaa ctggaagccg ggcaaatcat tctcggtggt
     4921 tcgttcaccc gcccggttcc ggcgcgtaag ggcgacacct tccacgtcga ttacggcaac
     4981 atgggttcca ttagctgccg ctttgtttaa ggagagaacg atggaaaaca gctttaaagc
     5041 ggcgctgaaa gcaggccgtc cgcagatcgg attatggctg gggctgagta gcagctacag
     5101 cgcagagtta ctggccggag caggattcga ctggttgttg atcgacggtg agcacacgcc
     5161 gaataacgtg caaaccgtgc tcacccagct acaggcgatt gcgccctacc ccagccagcc
     5221 ggtagtacgt ccgtcgtgga acgatccggt gcaaatcaaa caactgctgg acgtcggcac
     5281 acaaaccttg ctggtgccga tggtacaaaa cgctgacgaa gcccgtgaag cggtacgcgc
     5341 cacccgttat ccccccgccg gtattcgcgg tgtgggcagt gcgctggccc gcgcctcgcg
     5401 ctggaatcgt attcctgatt acctgcaaaa agccaacgat caaatgtgcg tgctggtgca
     5461 gatcgaaacg cgtgaggcaa tgaagaactt accgcagatt ctggacgtgg aaggcgtcga
     5521 cggcgtgttt atcggcccgg cggatctgag cgccgatatg ggttatgccg gtaatccgca
     5581 gcacccggaa gtacaggccg ccattgagca ggcgatcgtg cagatccgcg aagcgggcaa
     5641 agcgccgggg atcctgatcg ccaatgagca actggcaaaa cgctatctgg aactgggcgc
     5701 gctgtttgtc gccgtcggcg ttgacaccac cctgctcgcc cgcgccgccg aagcgctggc
     5761 agcacggttt ggtgcgcagg ccaccgccgt gaagcccggc gtgtattaat gccggatgcg
     5821 ctgcgcttat ccggcctaca ctcgcaccaa accgtaggcc ggataaggcg tttacgccgc
     5881 atccggcaaa aagccgtacc aaatcgcgga ccggataagg cgttacgccg catccgccat
     5941 aaaccttgtc gtaccctaca aaaatcccat tagaggaaga aaaatgagcg acacctcacc
     6001 tgccataccg gagagtatcg atccggcgaa tcagcataaa gcgctgactg ccggacaaca
     6061 ggcggttatt aagaagctat ttcgccgcct gagcgtcttt ctgttcgtgc tgtttatctt
     6121 ctcgttcctt gatcgcatca acatcggctt tgccggactc acgatgggac gcgacctcgg
     6181 tctgagcgcc accatgtttg gcctcgctac caccctgttc tatgccgctt atgtcatctt
     6241 cggcattccc agcaacatta tgctgagtat tgtcggtgcg cggcgctgga tcgccaccat
     6301 catggtgctc tggggcatcg cctctactgc caccatgttt gccactggcc ccaccagctt
     6361 gtacgtactg cgtatactgg ttggcattac cgaagccggc tttctgcctg gtattctgct
     6421 gtatttaacc ttctggtttc cggcctattt ccgcgcccgt gccaacgcct tgtttatggt
     6481 agcaatgccg gtaacgacag cgttgggctc gatcgtttcc ggctacattt tgtcgctgga
     6541 tggcgtcatg gcattaaaag gctggcagtg gctgtttttg ctggaaggct tcccgtcggt
     6601 attactcggc gtcatggtgt ggttctggct tgatgactca ccggacaaag ctaagtggct
     6661 gacgaaagaa gacaaaaaat gcctgcaaga gatgatggat aacgatcgtc tgacgctggt
     6721 tcagccagag ggagccatca gccaccacgc catgcaacaa cgcagtatgt ggcgggagat
     6781 cttcactccg gtggtgatga tgtataccct ggcgtatttc tgcctgacca acacacttag
     6841 tgcgatcagc atctggacac cgcagatcct gcaaagtttt aatcagggca gcagtaatat
     6901 caccatcggc atgctggccg ccgtaccgca gatttgtacc attctcggga tggtctactg
     6961 gagccgtcac tcagatcgcc gccaggaacg aaggcatcac accgcccttc cttatttgtt
     7021 cgctgccgca ggttggttac tggcttcggc aactgatcac aacatgatcc agatgctggg
     7081 gatcattatg gcttcgaccg gatcattcag cgcaatggcg attttctgga caacaccgga
     7141 tcagtccatc agcctgcggg cacgagcgat cggtattgcg gtgatcaacg ccactggcaa
     7201 cattggttca gcgttaagtc cgtttatgat cggctggttg aaagatttga ccggcagctt
     7261 taacagtgga ttgtggtttg ttgccgcgct gctggtgatt ggtgcgggga ttatctgggc
     7321 gattccaatg cagtcctccc gtccgcgagc gaccctgtaa ggaacgacga tgtgtgaccg
     7381 tcagattgcc aatattgata tcagcaaaga gtacgatgaa agcctgggca cggacgatgt
     7441 gcattatcag tcgttcgccc gcatggcggc attttttggc cgccatatgc tgccacatcg
     7501 ccacgaacag tactttcaga tgcatttcct caatagcgga cagattgagc tacagcttga
     7561 cgatcatcgc tactcggtgg aagcgcccct gtttgtcctg acgccgccgt ca
//
LOCUS       DADMKF010000178         7593 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.181, whole genome shotgun sequence.
ACCESSION   DADMKF010000178 DADMKF010000000
VERSION     DADMKF010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7593
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.181"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            137..823
                     /locus_tag="JNC24_003570"
                     /pseudo
     CDS             137..823
                     /locus_tag="JNC24_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554952.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication protein"
     gene            1156..2031
                     /locus_tag="JNC24_003571"
     CDS             1156..2031
                     /locus_tag="JNC24_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-regulated protein"
                     /protein_id="HAY9160234.1"
                     /translation="MRKFILISMITLASVSLGACRQNVTIKQDAPGQKAFLTDGQIYD
                     LHSGKIISSSELLADLATARHLIIGEKHDNAEHHQIELWLIQNLLIQRPQGSVLLEML
                     TSEQQPRVNQVKCWLKDNPVVRDSRVQELLNWQKGWSWEMYGDIVMQLLRGPYPLLNA
                     NIGREQMLALYKKNEFPKGKKSTAPVVQEALRETIISMHEGNLESQQLTSMLSIQQQR
                     DRYMARQLLSAPVPSLLIAGGYHASKSMGVPLHMEDLATGTHPVVLMLAEKGMNITVD
                     HADYVWFVAPDTTKR"
     gene            2039..2815
                     /locus_tag="JNC24_003572"
     CDS             2039..2815
                     /locus_tag="JNC24_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004095468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HAY9160235.1"
                     /translation="MMNILHFSQSVKWSSWFICSLLLHGLIFLAFIWRFSEVQPAMSP
                     APAIMLQWAEIIEAPSSPLSLPVGIAQQESAVTEEKQQTEDRQQRPVTEDSDATIEIT
                     RKKKSSDGEKKKTRPPRKIKAQTSDSNPTAVSSNAAPQALVESSRIAAPFNSDSTKRD
                     NSEASWESRVKGHLNRYKRYPGDARKRARTGTAVVTFTVNTEGTIVSSFLEISSGTLS
                     LDREAIAVLERAQPLPKPPPEILEGGLFKVKMPITFKLKE"
     gene            2984..5245
                     /locus_tag="JNC24_003573"
     CDS             2984..5245
                     /locus_tag="JNC24_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016536327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HAY9160236.1"
                     /translation="MNVIKLAIGSGILLLSCGAYSQSISEKTNSDKKGAAEFSPLSVS
                     VGKTTSEQEALEKTGATSSRTTDKNLQSLDATVRSMPGTYTQIDPGQGAISVNIRGMS
                     GFGRVNTMVDGITQSFYGTSTSGTTTHGSTNNMAGVLIDPNLLVAVDVTRGDSSGSEG
                     INALAGSANMRTIGVDDVIFNGNTYGLRSRFSVGSNGLGRSGMIALGGKSDAFTDTGS
                     IGVMAAVSGSSVYSNFSNGSGINSKEFGYDKYMKQNPKSQLYKMDIRPDEFNSFELSA
                     RTYENKFTRRDITSDDYYIKYHYTPFSELIDFNVTASTSRGNQKYRDGSLYTFYKTSA
                     QNRSDALDINNTSRFTVADNDLEFMLGSKLMRTRYDRTIHSAAGDPKANQESIENNPF
                     APSGQQDISALYTGLKVTRGIWEADFNLNYTRNRITGYKPACDSRVICVPQGSYDIDD
                     KEGGFNPSVQLSAQVTPWLQPFIGYSKSMRAPNIQEMFFSNSGGASMNPFLKPERAET
                     WQAGFNIDTRDLLVEQDALRFKALAYRSRIQNYIYSESYLVCSGGRKCSMPEVIGNDW
                     EGISDEYSDNMYIYVNSASDVIAKGFELEMDYDAGFAFGRLSFSQQQTDQPTSIASTY
                     FGAGDMTELPRKYMTLDTGVRFFDNALTLGTIIKYTGKARRLSPDFEQDEHTGAIIKQ
                     DLPQIPTIIDLYGTYEYNRNLTLKLSVQNLMNRDYSEALNKLNMMPGLGDETHPANSA
                     RGRTWIFGGDIRF"
     gene            5314..6489
                     /gene="senB"
                     /locus_tag="JNC24_003574"
     CDS             5314..6489
                     /gene="senB"
                     /locus_tag="JNC24_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001020413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterotoxin production-related protein TieB"
                     /protein_id="HAY9160237.1"
                     /translation="MNIFTLSKAPLYLLISLFLPTMAMAIDPPERELSRFALKTNYLQ
                     SPDEGVYELAFDNASKKVFAAVTDRVNREANKGYLYSFNSDSLKVENKYTMPYRAFSL
                     AINQDKHQLYIGHTQSASLRISMFDTPTGKLVRTSDRLSFKAANAADSRFEHFRHMVY
                     SQDSDTLFVSYSNMLKTAEGMKPLHKLLMLDGTTLALKGEVKDAYKGTAYGLTMDEKT
                     QKIYVGGRDYINEIDAKNQTLLRTIPLKDPRPQITSVQNLAVDSASDRAFVVVFDHDD
                     RSGTKDGLYIFDLRDGKQLGYVHTGAGANAVKYNPKYNELYVTNFTSGTISVVDATKY
                     SITREFNMPVYPNQMVLSDDMDTLYIGIKEGFNRDWDPDVFVEGAKERILSIDLKKS"
     gene            complement(7015..7476)
                     /locus_tag="JNC24_003575"
                     /pseudo
     CDS             complement(7015..7476)
                     /locus_tag="JNC24_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611683.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2108 a         1772 c         1801 g         1912 t
ORIGIN      
        1 gtcagagagc gtctgagagg gcacgtacaa cgggagttat ggtaaatgga tcgggttttc
       61 gggaaggttc gacaggattt gccgttgggt gtagtgtaag cgactgaaaa acaaacgccc
      121 ctgaaatcat gtccagatcc ggcagaacca gcctcatgcg cagttctggc tggtttttga
      181 cgttgatcgc gagggggcag cgattgactg gagcgaccgg aacgctcccg cgccgaacat
      241 caccgtaaaa aatcctgtga acggtcatgc tcacctgctc tacgcgctca atatcgccgt
      301 gagaaccgcg ccggacagct cggttaaggc gctgaaatac gccgctgcga ttgaacgttc
      361 gctgtgtgag aaactttgtg ctgatgtgaa ctacagcgga ctgatttgta aaaatccgtt
      421 ccacctggaa tggcaggtga tggagtggcg agaggaagcc tataccctcg atgaactggc
      481 tgattatctc gatttgagcg cctcagcgcg ccgtagcatc gataaacatt acgggatggg
      541 gcgaaactgc cacctgttcg aaatgacgcg caaatgggct tacagggcga ttcgtcaggg
      601 ctggcccgaa ttctcacagt ggcttgatgc cgtgattcag cgtgtcgaaa tgtacaacgc
      661 atcgcttccc gttccgcttt caccgcctga atgtcgggct attggcaaga gcattgcgaa
      721 atacacgcac aggaacttca cgccggaaac tttcgcacag tatgtggctg atacgcacac
      781 attaacgtac gttttcgttc cattggccct caccccccga taacgaccca ggtagcgcgc
      841 cgtattcccc ccgccggccg ttttcttcga catgtacatc aggcgcggaa ctcatcgggg
      901 cgcgggaatg aaatcccatt cagactccgg atagattcaa gcaagcccac ttatcgtcaa
      961 aatcggtgtt gcaaaaacgg gagtgaccat agatccgttt tccaagcgga ccccataata
     1021 tcggctggta aacatcactc gctagtgcat tctttttgtt ggtaatgata atcatttaca
     1081 ttcattcttt gatgttttat cataattaat gttttgtcaa taaatatatg gaaacatatt
     1141 gtttcagatg ggaatatgag aaagttcatc cttatttcta tgataacgtt agctagtgtt
     1201 agtctgggag cctgcagaca gaatgtcacc ataaagcagg atgcgccagg gcagaaagct
     1261 ttcctgactg acgggcagat ttacgatttg cacagtggca aaattatttc tagcagcgag
     1321 cttttggcgg atcttgctac cgctcgacat cttattattg gtgaaaaaca cgataatgcc
     1381 gagcatcatc agattgagct ttggcttatt caaaatttgc tcatccagcg accacagggg
     1441 agtgtgttgc ttgagatgtt gacgtcagag caacagcccc gagttaacca agttaaatgt
     1501 tggctgaaag ataatcctgt ggttcgagat agccgtgttc aggaactact taactggcaa
     1561 aaagggtggt cctgggagat gtatggcgac atcgtcatgc agctattacg tgggccttac
     1621 ccgctgctca atgccaacat tggccgggag cagatgttgg cactgtacaa aaaaaacgaa
     1681 tttcctaaag gaaaaaaatc cactgcacct gtggtacaag aagctctgcg tgaaaccatc
     1741 atatctatgc atgaaggcaa ccttgaaagc cagcaactga cgtcaatgct ctctattcag
     1801 cagcagcggg atcgctacat ggcccgccag cttctgagtg ccccggtacc gtcgttgttg
     1861 attgcaggtg gttatcacgc gtcaaaaagc atgggcgtac cgttacacat ggaagatctt
     1921 gccaccggca cccatccagt ggtgttgatg cttgcagaaa aaggaatgaa catcacagta
     1981 gatcatgctg actacgtgtg gtttgtagcg ccagatacaa caaaaaggta aaccatggat
     2041 gatgaatatt ctccacttct cacagtcggt aaaatggagc agctggttta tctgttctct
     2101 actgcttcat ggactgattt tccttgcgtt tatctggcgt ttttccgaag ttcagcccgc
     2161 tatgtctcct gcaccggcga tcatgctgca atgggcagaa attatagagg ctccttcttc
     2221 tccactttcg cttccggtgg gaatcgctca acaggagtcc gcagtgacag aagaaaagca
     2281 acaaactgag gacagacaac aacgtcctgt cactgaagac agtgatgcaa ccattgaaat
     2341 aactaggaag aaaaagtcat ccgatggaga aaagaaaaag acacgtccac ctcgtaagat
     2401 aaaggctcaa accagcgaca gcaaccccac agccgtttcc agtaatgctg cacctcaggc
     2461 tctggtcgaa tcgtcccgta ttgctgcgcc ttttaacagt gactcaacaa agcgagataa
     2521 ttctgaggca tcttgggaaa gcagagtaaa aggccaccta aaccgttata aacgttaccc
     2581 tggcgatgcc agaaaacgtg cacgaaccgg cacagccgtc gtgactttta ccgtaaacac
     2641 ggaaggcact attgtcagta gtttcttgga aatctcttca ggaacccttt ctcttgaccg
     2701 cgaagctatt gctgttttag aacgcgctca accacttccc aaaccaccgc ctgaaatatt
     2761 ggagggagga ttatttaaag taaaaatgcc gattaccttt aagttgaagg agtaaaggga
     2821 taattggccg ttcaagttca tttaactgaa atttaatttt catttttatg ctgaacattt
     2881 tatcaaactt ttccactgtc gaaaaaatca aaaagacagt agaacataat taataaacaa
     2941 ttagttatac ttatctttat taatattata cccaaggaaa tttatgaacg ttataaaact
     3001 ggctatcggc tcaggcatat tattgctcag ctgcggtgct tactcacaat ccatcagtga
     3061 aaaaactaat tccgacaaaa aaggagcggc agaattcagt ccgctcagcg tttctgtcgg
     3121 gaagacgacc agtgagcagg aagctctcga gaaaacaggc gcgaccagtt cccggacaac
     3181 ggacaaaaac ctgcaatcac ttgacgcaac agtgcgtagt atgcctggta cttatactca
     3241 aatagatcct ggtcagggag caatcagtgt gaatattcga ggcatgagcg gatttggtcg
     3301 tgtaaacact atggtcgatg gtattaccca gagtttttac ggaacctcta cctccggaac
     3361 aacgacgcat ggttcaacta acaatatggc tggcgtactt atagatccta acttactggt
     3421 agcagttgat gttacacgcg gtgacagcag tggctctgaa gggatcaacg cccttgccgg
     3481 tagtgcaaat atgcgtacta ttggcgttga cgatgtaata tttaacggta atacatatgg
     3541 ccttcgttca cgtttctctg tcggtagtaa tgggctggga cgcagcggaa tgatcgccct
     3601 tggtggaaaa agcgacgctt ttacggatac gggaagcatt ggcgttatgg ctgctgtgag
     3661 cggcagttct gtgtactcta atttctcaaa tggttctgga attaacagca aagagtttgg
     3721 ttatgataaa tatatgaagc agaaccccaa atcccaactg tataaaatgg atatcagacc
     3781 agacgaattt aacagcttcg aactttccgc tcgaacctat gaaaataaat ttacacgtcg
     3841 tgatataacc agtgacgact attacattaa atatcattac accccttttt ctgaattaat
     3901 tgactttaac gtaacggcca gtaccagtcg cggtaatcaa aagtatcgtg atggctcgct
     3961 gtatactttc tacaaaacct cagcgcaaaa tcgttctgac gcgctggata tcaacaatac
     4021 cagccggttc actgtcgcgg acaatgacct ggagtttatg ctgggcagca aactgatgcg
     4081 tacccgctat gaccggacca ttcactcagc ggcgggcgac ccgaaagcga atcaggaatc
     4141 gatcgagaac aatccgttcg caccctccgg ccagcaggat atttcagcgc tgtataccgg
     4201 gctgaaggtt acgcgcggca tctgggaggc agatttcaat ctcaactaca cacgtaacag
     4261 gatcacaggg tacaagcccg cctgcgattc acgcgttatc tgcgtgccac agggtagcta
     4321 cgatattgac gataaagagg gtggcttcaa cccttcagtt cagctttctg ctcaggtaac
     4381 accatggctt cagccgttca ttggctacag caaatccatg cgcgccccga acatccagga
     4441 gatgttcttc tctaattcag gaggcgcatc catgaaccca ttcctgaagc ctgaacgtgc
     4501 agaaacctgg caggcgggtt ttaacattga taccagagat ttactggtcg aacaggatgc
     4561 cctgcgcttt aaggctctgg cgtaccgcag caggatccag aactacatct acagcgagtc
     4621 ttatctggtt tgttctggag gtcgtaaatg cagtatgcct gaggtgattg gcaatgactg
     4681 ggagggcatt agcgatgaat acagcgacaa tatgtacatc tacgttaact cggcaagcga
     4741 cgttatcgca aagggcttcg aactggagat ggattatgat gcaggttttg cttttggccg
     4801 actctctttc agccagcagc aaacagacca gccaacctcc atcgccagca cctactttgg
     4861 cgcaggggat atgaccgaac tgcccagaaa atacatgacg ctggatactg gtgttcgctt
     4921 cttcgataac gcgttgaccc tgggcactat cataaaatac acaggcaagg ctcgtcgcct
     4981 gtcgcctgat tttgagcagg acgaacatac cggcgcaata atcaaacagg atttgccgca
     5041 gatcccaacg attatcgatc tctatggtac ttacgagtac aaccgcaacc taacactgaa
     5101 actttcggta caaaacctga tgaacagaga ttattcggag gcgctgaata agctcaacat
     5161 gatgccaggt cttggtgacg agacccaccc agccaattcc gcgcgtggca gaacatggat
     5221 atttggcggg gacattcgtt tctgatgaca atttccgcta ggttcagaca atacgttttc
     5281 tctcttatgt caattttatt gcaggaacga aaaatgaata ttttcacttt atccaaagca
     5341 ccgctatacc tgttaatttc actattttta cccacgatgg ccatggctat cgatccacct
     5401 gaacgcgaac tttcgcgatt tgccctgaaa acgaattacc ttcagtcccc tgatgaaggc
     5461 gtctatgaac tggcgtttga taatgccagt aaaaaggtgt ttgcagcagt caccgatcgt
     5521 gtaaatcgtg aagccaataa aggctatctg tattcgttta attcagattc gctgaaagtc
     5581 gaaaataaat acacgatgcc ataccgggca ttttcgctgg cgataaatca ggataaacat
     5641 cagctctata tcggacacac ccagtcagcg tccctgcgta tcagtatgtt tgacacccca
     5701 accggcaaac tggtaagaac cagcgacagg ttaagtttta aagcggcaaa cgctgcagat
     5761 tcgcgttttg agcattttcg ccatatggtt tacagccagg attccgatac cctgtttgtg
     5821 agttatagca atatgctgaa aacggccgag ggcatgaagc ctctgcataa gctgttaatg
     5881 ctcgacggga cgacgcttgc cttaaaaggc gaggttaagg atgcttacaa aggtacagcg
     5941 tatggtctga cgatggatga aaaaacacag aaaatctacg ttggcggaag agattacatc
     6001 aacgaaattg atgcgaaaaa tcagacgctg ctgcgtacca tcccgttgaa agatccgaga
     6061 ccacaaatca caagtgtgca gaatctggcg gtggactccg cttctgaccg tgcctttgtg
     6121 gtggtattcg accatgacga tcgttccggt acaaaagatg gactctatat ttttgactta
     6181 cgcgacggta aacagcttgg ctatgtgcac acaggagccg gagctaacgc ggtgaaatac
     6241 aatccgaaat ataacgaact gtatgtcacc aacttcacta gcggcaccat cagcgtagtg
     6301 gatgccacca aatacagcat cacccgtgaa tttaacatgc cggtctaccc aaaccagatg
     6361 gtgttgtcgg acgatatgga taccctttac attggcatca aagaaggctt taaccgcgat
     6421 tgggatcctg atgtgtttgt ggaaggagct aaagaacgta ttctgagcat tgatttgaaa
     6481 aagtcgtgat cggggggaaa aaacatggga taaaaactcc gttattccac taaactggcc
     6541 ggactaggaa aagcaaacat ctctgtcctg ccggtacgcc tgttacgccg ctccatgagg
     6601 ctgaggaatc tccagaaaac gagacaggca cagcattgta cgatcaactg atttgtgcca
     6661 ataattaaat gaaatcacag ctatgcctgt tcatggggtt ctaggccgga accccagatt
     6721 tttccgattg tttgttttct aactgattat tcagtagttt tagagggctg acgctcagtg
     6781 gaacgaaaac tcacgttaag aggttttgac tatgataaaa gtcaaagtag gggtgagttt
     6841 ggatatggaa attattgtac tataaggaga gcatgaaaac ccctggggca gtcactgaat
     6901 gagggaccag cagcggtaaa ataaccagca taatttctgc atggtcgatt tttataccgt
     6961 gacaggctgt tttttatact tggggtcagt ttggatatcg aaaattattg tacgttaagg
     7021 ttgttttttg accttgtcga tcttaactga tctcaaacga cgaaacttac ctgaagtcgg
     7081 tatccggctg tttttccaga tataatcgcc tgtcagattg atatgttccc aacctaatgg
     7141 tgataaatgc gataatagtt gagcatttat tttcatgccc ttacgcttca gagcatcaac
     7201 cgctctttcc atgtaaactg tgttccataa ggaaatagcc gccgtcaata aagtcaatcc
     7261 actggcgcga taactttgat tttccggttt ccgatctcta atctccccca atcgatgcat
     7321 gaatacagca cgggccagag cattacgggc ttctcctttg ttcagcccgg cctggacacg
     7381 tcgccgcaat gccggatccc gaaaccagtc cagcataaac agtgttcgtt ctatacggcc
     7441 aatctctctc agagctttag ccaacccatt ttgtttgtaa gcgtgcagca agaaccgtat
     7501 tgacgggaat gtgttattca gtcggcagtg ctacgcgcca ggggagcagt tcgccgaccc
     7561 ggtttatcgg ccagtcggct atgacgtcaa gga
//
LOCUS       DADMKF010000179         7575 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.182, whole genome shotgun sequence.
ACCESSION   DADMKF010000179 DADMKF010000000
VERSION     DADMKF010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7575
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.182"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            <1..300
                     /locus_tag="JNC24_003576"
     CDS             <1..300
                     /locus_tag="JNC24_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9160238.1"
                     /translation="KNSHQKKIYDYIDIIIEDYENIKENAYLVDPKINYTIDLNIEDS
                     SSEEFLFDNSLEKDENSSDNCFEVEKYNAYEAYNSENLYFTREEYTYDYDLLNAI"
     gene            complement(688..1064)
                     /gene="rnpB"
                     /locus_tag="JNC24_003577"
     ncRNA           complement(688..1064)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="JNC24_003577"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(1097..2242)
                     /gene="garK"
                     /locus_tag="JNC24_003578"
     CDS             complement(1097..2242)
                     /gene="garK"
                     /locus_tag="JNC24_003578"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HAY9160239.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSVPVADG
                     GEGTVEAMIAATQGAERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESSATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYADVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTDDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNAG"
     gene            complement(2339..3229)
                     /gene="garR"
                     /locus_tag="JNC24_003579"
     CDS             complement(2339..3229)
                     /gene="garR"
                     /locus_tag="JNC24_003579"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HAY9160240.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGEIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLLLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            complement(3259..4029)
                     /gene="garL"
                     /locus_tag="JNC24_003580"
     CDS             complement(3259..4029)
                     /gene="garL"
                     /locus_tag="JNC24_003580"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HAY9160241.1"
                     /translation="MNNDVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAEQAVASTRYPPEGIRGVSVSHRTNMFGTVADYFAQSNKNITILVQIESQQGVDN
                     VDAIAATEGVDGIFVGPSDLAAALGHLGNASHPDVQKAIQHIFNRASAHGKPSGILAP
                     VEADARRYLEWGATFVAVGSDLGVFRSATQKLADTFKK"
     gene            complement(4045..5379)
                     /locus_tag="JNC24_003581"
     CDS             complement(4045..5379)
                     /locus_tag="JNC24_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY9160242.1"
                     /translation="MILDTVDVKKKGVHTSYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGGIGFVLTALWIKLIHNPTDHPRMSAEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     VSTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            5754..7325
                     /gene="garD"
                     /locus_tag="JNC24_003582"
     CDS             5754..7325
                     /gene="garD"
                     /locus_tag="JNC24_003582"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HAY9160243.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHTPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPAPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLVGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPASELVVGVQCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMATRTELANRWFDLMDINAGTIATGEESIEEVGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            7474..>7575
                     /gene="prlF"
                     /locus_tag="JNC24_003583"
     CDS             7474..>7575
                     /gene="prlF"
                     /locus_tag="JNC24_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PrlF"
                     /protein_id="HAY9160244.1"
                     /translation="MPANARSHAVLTTESKVTIRGQTTIPAPVREALK"
BASE COUNT         1931 a         2027 c         1817 g         1800 t
ORIGIN      
        1 aagaattctc atcagaaaaa aatatatgat tacatcgata taatcattga agattatgaa
       61 aatatcaaag aaaatgctta tctggtcgat ccaaaaatta attacactat agatttaaac
      121 atagaagact ctagctcaga ggaattcctt tttgataata gcctcgagaa agacgaaaat
      181 tcttcggaca attgctttga ggtcgaaaaa tataacgcat atgaagcata taactcagag
      241 aatctatatt ttaccagaga ggaatatacg tatgattacg accttttaaa tgcaatatag
      301 acataataac ttcttacaaa atctataatt aataccgtag atattcattc atcttaccct
      361 gtgcaccaaa agcaaaaact cactgacgcg ggtttgaaac tgatggcaac cgcaaaaatg
      421 cctgatgcgc tacgcttatc aggcctacgc catctctaca atatattgaa tttgcgtgct
      481 tttgtaggca ggataaggcg ttcacgcagc atccggcatg aataaagcgc actcaacaat
      541 ctgaaacccg ccggaacggt ttattacgta catcaggtga aactgaccga taagccgctt
      601 tcttttgggt atagtgtcgt ggacagtcat tcatctttct gcccctccaa aagcaaaaac
      661 ccgccgaagc gggtttttac gtaaatcagg tgaaactgac cgataagccg ggttctgtcg
      721 tggacagtca ttcatctagg ccagcaatcg ctcactggct caagcagcct acccgggttc
      781 agtacgggcc gtaccttatg aacccctatt tggccttgct ccgggtggag tttaccgtgc
      841 cacggactgt taccagccgc gcggtgcgct cttaccgcac cctttcaccc ttacctgatc
      901 ccgcttgcgc gggccatcgg cggtttgctc tctgttgcac tggtcgtggg tttccccccc
      961 aggcgttacc tggcaccctg ccctatggag cccggacttt cctcccctcc gcccgtctcc
     1021 cccgaagagg acgacgacga agcggcgact gtctggtcag cttcggcgcg cagtatagag
     1081 ggtttgcgcg cccttgtcac cccgcgttgc gcattccaat cgccagtgtc gcggcgatat
     1141 tacgtgaagc acggcagata ttgtcataag ccccgcggaa tgcttcgtcc aacgtaccta
     1201 tgctggtcaa tacgctgaag accgcatcga tgccatgctg atgtacaacg ccaacatcat
     1261 cggtcaggct acccgcaatg ccaatcaccg gtttatggta cttcttcgcc acgtttgcaa
     1321 cgccaatcgg taccttgccg tgaatactct ggctgtcaat acgcccttca ccggtgatca
     1381 ccagcgtaca atcgtgaata tgttcttcca gattcagcgc cgtagtgacg atttcaatac
     1441 cacttttcag ttccgcacca agaaacgcca tcagcgccgc gcccataccg cccgcagctc
     1501 ctgcgccggg gacatctttc acatcaacat gcagcgcttt tttaatgaca tcggcatagt
     1561 gagagaggtt attgtccagc tcaacaatca tcgcttcact ggctcccttt tgtgggccaa
     1621 agatgcgcga tgcgccgtta tcgcccacca gcggattggt gacatcacaa gcgacgcgaa
     1681 tgacgcaatc ttttaagcgc ggatcgaggc cggaaatatc aatatcattc agagtattaa
     1741 gactaccgcc gccaaaacca atttcattgc cgttggcgtc gcataatttc gcccccagcg
     1801 cctgtaccat gcctgcgccg ccatcatttg tagcgctgcc gccaatgccg ataataatgt
     1861 ttgttgcact gctctccagc gcctgcagga ttaactcgcc tgtgccgcgt gaagtggtta
     1921 cgagtggatc gcgtttttcc gcaggtacca gctccagccc actggccgcc gccatttcaa
     1981 taaacgcggt tttgccatcg ccggagatcc cccaactggc attcactttc tcgcccagcg
     2041 gccctgtaac ccaggcgtga cgttcagccc cctgggtggc tgcaatcatc gcttccaccg
     2101 ttccttcacc accgtcggca accggaacag aaacatactg tgcatcagga aaaatttccc
     2161 gaaatccttt ttctatcgcc tgcgcaacct cgctggcaga taaactttct ttataagagt
     2221 ctggggcgat tacgattttc atacctatgc ctgttaccac atgacgccgg agggcgtttc
     2281 tcttattcgg cctagattcc aggcccggat tgcaatacac catccgggca cgacgtcatt
     2341 aacgagtaac ttcgactttc gccagttttt cgtagtagca cgccagggcg ctatgatccg
     2401 ccgttcctaa accatctgca cgcagtgcct gcatcatctc cataaccgca gctgtgagca
     2461 gcagttgtgc gccgacgccg tgagaagtat ccagcgcatt cgccagatcc ttaatatgca
     2521 gatcaatgcg gaagcccggc ttgaagttgc ggtccatcac catcggcgct ttggcatcca
     2581 gcacggtact gcccgccagc ccaccgcgaa ttgcctgata aaccaggtcc gggttaacgc
     2641 ccgctttggt tgccagcgtt aacgcttctg acatcgcagc aatattcagc gccacaatga
     2701 cctgatttgc cagtttggtg acgttacctg caccgatttc cccggtatgc accacggaac
     2761 ccgccatcgc tttcatcaaa tcatagtatt tgtcgaaaat agccttgtcg ccgcccacca
     2821 tcactgacag cgtaccgtcg atggctttcg gttcaccgcc gctcaccgga gcatccagca
     2881 tatcaatgcc tttcgctttc agcgcttcgc tgatttcacg gcttgccagc ggtgcgatag
     2941 aactcatatc gatcaatacc gtacctggct tcgcgccttc aataatgcca ttctcaccca
     3001 gcgccacctc tttcacatga ggggagtttg gcagcatggt tatgatgacg tcgcactgtt
     3061 cagcgatcgc tttagccgta gacgctgttt ctgcacctgc agcaatcacg tcagcaatag
     3121 cttctgggtt acggtcagca accaccagcg agtaacctgc tttcagaagg tttttactca
     3181 ttggtttacc cataatcccc aggccaataa aaccaacttt catagtcata tcaaccatct
     3241 ctcttgttgc ggtggtggtt attttttaaa ggtatcagcc agtttctgag tggcagagcg
     3301 gaagacgcca agatcgctgc cgacagccac aaacgtcgcg ccccattcca gataacgacg
     3361 cgcatcggct tcgaccggcg cgagaatacc gctgggtttg ccgtgcgcgc tggcacggtt
     3421 aaaaatatgc tgaattgctt tttgtacatc cggatgcgag gcattgccga gatggcccaa
     3481 tgccgcagcc agatcgctgg ggccgacgaa gataccgtct acaccttcgg tagcggcaat
     3541 agcatcgacg ttatctacgc cctgctgact ttctatctgg accagaatag taatgttctt
     3601 gttcgactga gcgaaataat ccgccacggt gccaaacata ttggtgcggt gagaaacgga
     3661 gacgccgcga atgccttccg gcgggtaacg agttgatgcc accgcctgct ctgcttcctc
     3721 ttttgtttct acaaaaggaa tcaggaagtt atagaaaccg atatccagaa gacgcttaat
     3781 aattaccggc tcgttggtcg gcactcgcac tactggcgcg ctggcgctgc ctttcagggc
     3841 cattaactgc ggaataaacg tggaaatatc gtttggcgca tgttcgccat ccagcaccag
     3901 ccagtcaaac ccagccaaac caagaacttc agtgctaatc gggttagaga gtgctgacca
     3961 gcaaccaatt tgtacctgtt tcgcagccag tgcggctttg aatttattcg ggaaaacatc
     4021 gttattcatc gcttatacct ttgcttattt ctgcaattcc atacgtttaa tgtcgccaac
     4081 tacgaagagg tagcagacca tcgccatcag cgctgaacat cccacgaaaa ccagtgctgc
     4141 attgaaggag tgcagttcac ttaccaggta gccaatcacc agtggagtga caatggaggc
     4201 aacattgcca aagacgttaa agacgccgcc acagaggcca acaatctctt tcggcgcggt
     4261 gtcagaaatc accggccagc ccagagcacc aaatcctttg ccaaaaaaag ccagcgccat
     4321 cagcatgacc accagcgtgg tgttgttggt gtagttacat aagatgatgg tggaaaccag
     4381 caacattccc agcacaatcg gtagcttacg tgccagggtc agggataaac cgcgtttgat
     4441 cagataatcc gagaagacac ctcccagcac gccgcccgca aaaccacaca gtgccggaat
     4501 cgaggcgacc agacccactt tcagaatcga catgcctttt tcctgcacca gataaatcgg
     4561 gaaccaggtg aggaagaacc aggtgatggt gttgataaaa tattgtccga aaaatacgcc
     4621 caacatcatg cggttagaga gcaattgctt gatgtaatgc agtttgggac cgcttgctgc
     4681 cgcgctgccc ggctttttgt ggtccatatc gaccaccgcg ccattttcag agataaactt
     4741 cagctcttcc gcagacatac gtgggtgatc tgtcgggtta tgaatcaact tgatccacag
     4801 cgccgtcagc acaaaaccaa tcccccccat aacggtgaag acgtgctccc agccccaagc
     4861 gaaagtcagc cagccaagca gcggcgaaaa gagcgccagc gagaaatatt gcgccgagtt
     4921 aaagatggcg gaggcagtac cacgttcttt cgtcgggaac caggcggcga caatacgggc
     4981 gttcgccggg aatgatggcg cttccgagaa ccccagcata aagcgcataa agaacatgga
     5041 gatccctgcc caggccagcg ggaacatatc aacaaagcct tgcaggaagg tgaacagcga
     5101 ccagaaaaag aggctgtagg tgtaaacttt tttcgagcca aacttatcaa gcaaccagcc
     5161 gccggggatt tgcatcagca agtaggccca gccaaaagca gagaagatgt aacccatcga
     5221 aaccgcactt aactgcaact cttttgccac ttcggtacca gcaatagaca gcgttgcacg
     5281 atcggcgtag ttaacggcgg taacaataaa aataatcagt aatattaaat agctggtatg
     5341 cacgcctttc ttttttacgt caacggtgtc cagaatcatt ttatttacct cgggtactta
     5401 tgctgatttt tattattatg gggaaggtgt tatttatgag ttttatttat gccgttacga
     5461 caatgaactc gggaattagt ataagcagcg ggagaataat aatcattgtg caaatgctaa
     5521 tttaatcagt actatttaaa tattattttg agcatatgca cataaggttg cgcgctaaag
     5581 cacagatttg cgctttacct taccgggcgg cactgcaatc cctgaaatga ttgacattga
     5641 tcacatttct gcgtttaaac tcctgacatt cttatttcac ccaatgaagt catttatttt
     5701 taaatgagac cagatcctca ttttaataac ccctggctgg agaatattgc acaatggcca
     5761 acatcgaaat cagacaagaa acgccaactg cgttttatat aaaagttcac gacacagata
     5821 atgtggcaat tattgttaat gataatggcc tgaaagcagg aacgcgtttt ccggatgggc
     5881 tggaattaat tgaacatact ccccaggggc ataaagtcgc attgctggac attccagcta
     5941 atggtgaaat tattcgttat ggcgaagtga ttggttacgc cgtgcgcgca atcccacgcg
     6001 gaagctggat cgacgaatca atggttgtac taccggaagc gccgccgtta cacacgctgc
     6061 cactggcaac caaagtcccg gcccccttac cgccgctgga aggatacacc tttgagggct
     6121 atcgcaatgc cgatggcagc gtgggcacca aaaacctgct cggcatcacc accagcgtcc
     6181 actgtgtggc aggcgtggtg gactatgtag taaaaatcat tgaacgcgat ctgctaccga
     6241 aatacccgaa cgtcgatggc gtggtggggc tgaatcattt gtacggctgt ggcgtggcga
     6301 ttaacgcacc ggcggcagtt gtgcctattc gtaccatcca caatatttcg ctgaacccta
     6361 actttggcgg cgaagtaatg gtgattggtc tgggttgtga aaagttgcag cctgagcgcc
     6421 tgctggtcgg cacggatgat gtgcaagcta ttccagtaga aagcgccagc attgtcagtt
     6481 tgcaggatga aaagcatgtc ggctttcagt ccatggtcga ggatattttg caggtcgccg
     6541 aacgccatct acaaaaactg aatcaacggc agcgagaaac ctgcccggct tcagaactgg
     6601 tcgttggtgt gcagtgcggt ggcagcgatg cgttttctgg tgtaacggca aacccggcgg
     6661 ttggctatgc gtctgatcta ctggtgcgct gcggcgcaac ggtgatgttt tcagaagtaa
     6721 cggaagtgcg tgacgcgatc catctgctga caccacgcgc agtgaacgaa gaggtcggca
     6781 aacggctgct ggaggagatg gagtggtacg ataactatct caatatggga aaaaccgacc
     6841 gcagcgccaa cccttcgccg ggcaacaaga aaggcggtct ggcaaatgtg gtggagaagg
     6901 cactcggctc cattgctaaa tcgggtaaaa gtgcaattgt tgaagtgctg tcgcccggtc
     6961 aacgcccgac taaacgcgga ttaatttacg ccgcgacgcc agccagcgat tttgtctgtg
     7021 gcacgcaaca ggtggcttcg ggtatcaccg tacaagtgtt tacgaccggc cgtggtacgc
     7081 cgtacggcct gatggcggta cccgtaatta aaatggcaac ccgcaccgag ctggcgaacc
     7141 gctggtttga tttaatggat attaacgcgg gcactatcgc taccggcgaa gagagcattg
     7201 aagaggtggg ctggaagttg ttccacttta ttctcgacgt cgccagcggg aagaagaaaa
     7261 ccttctcgga tcaatggggg ctgcataacc agctggcggt gtttaacccg gcaccggtga
     7321 cctgatttct tttggaacgt ccctcgcaaa acatggcctt agtgccatgt ttttattgtt
     7381 taaagccccc acgtccatta ataatgcatt tgcattacct tagttcaagc ttataatttg
     7441 agcagaaaac aggctgtaaa aggacaatga atcatgcccg ctaatgctcg ctctcacgct
     7501 gtactgacca ctgaatcaaa ggtcacgata cgcggacaaa caactatccc cgcgccagtg
     7561 cgtgaggcct taaaa
//
LOCUS       DADMKF010000180         7491 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.183, whole genome shotgun sequence.
ACCESSION   DADMKF010000180 DADMKF010000000
VERSION     DADMKF010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7491
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.183"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(16..522)
                     /gene="mug"
                     /locus_tag="JNC24_003584"
     CDS             complement(16..522)
                     /gene="mug"
                     /locus_tag="JNC24_003584"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HAY9160245.1"
                     /translation="MVEDILAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVIYQA
                     GFTDRQLKPQEAQHLLDYRCGVTKLVDRPTVQANEVSKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKQAYEQGFSQRGAQWGKQTLTIGSTQIWVLPNPSGLSRVSLEKLVEAYRELD
                     QALVVRGR"
     gene            647..722
                     /locus_tag="JNC24_003585"
     tRNA            647..722
                     /locus_tag="JNC24_003585"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:680..682,aa:Ile,seq:cat)
     gene            complement(776..1540)
                     /gene="nfeF"
                     /locus_tag="JNC24_003586"
     CDS             complement(776..1540)
                     /gene="nfeF"
                     /locus_tag="JNC24_003586"
                     /EC_number="1.16.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase"
                     /protein_id="HAY9160246.1"
                     /translation="MNNSPRYPQRVRNDLRFRELTVLRVERISAGFQRIVLGGEALDG
                     FTSRGFDDHSKLFFPQPDAHFVPPTVTEEGIVWPEGPRPPSRDYTPLYDELRHELAID
                     FFIHDGGVASGWAMQAQPGDKLTVAGPRGSLVVPEDYAYQLYVCDESGMPALRRRLET
                     LSKLAVKPQVSALVSVRDNACQDYLAHLDGFNIEWLAHDEQAVDARLAQMQIPADDYF
                     IWITGEGKVVKNLSRRFEAEQYDPQRVRAAAYWHAK"
     gene            1828..2451
                     /locus_tag="JNC24_003587"
     CDS             1828..2451
                     /locus_tag="JNC24_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAY9160247.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHSRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDITDAQIKKIIAVIDHAAFDITQLD"
     gene            complement(2558..4078)
                     /locus_tag="JNC24_003588"
     CDS             complement(2558..4078)
                     /locus_tag="JNC24_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HAY9160248.1"
                     /translation="MSSHPYVTQQNTPLADDTTLMSTTDLQSYITHANDTFVQVSGFT
                     LQELQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VREGKISGYMSIRTRATDEEIAAVEPLYKALNAGRTSKRIHKGLVVRKGWLGKLPSLP
                     LRWRARGVMTLMFILLAAMLWFVAAPVVTYFLCVLVVLLASACFEWQIVRPIENVARQ
                     ALKVATGERNSVEHLNRSDELGLTLRAVGQLGLMCRWLINDVSSQVSSVRNGSETLAK
                     GTDELNEHTQQTVDNVQQTVATMNQMAASVKQNSATASAADKLSITASNAAVQGGEAM
                     TTVIKTMDDIADSTQRIGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGE
                     VRHLASRSANAANDIRKLIDASADKVQSGSQQVHAAGRTMEDIVAQVKNVTQLIAQIS
                     HSTLEQADGLSSLTRAVDELNLITQKNAELVEESAQVSAMVKHRASRLEDAVTVLH"
     gene            4385..5875
                     /gene="ygjG"
                     /locus_tag="JNC24_003589"
     CDS             4385..5875
                     /gene="ygjG"
                     /locus_tag="JNC24_003589"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HAY9160249.1"
                     /translation="MITEFVFIPIFAIAAGVAQSLQYLNRYHVIREPPEHILNRLPSS
                     ASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKEHVNPGFLEYRKSVTAGGDYGA
                     VEWQAGSLNTLVDTQGQEFIDCLGGFGIFNVGHRNPVVVSAVQNQLAKQPLHSQELLD
                     PLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKLAKAYQSPRGKFTFIATSGAFH
                     GKSLGALSATAKSTFRKPFMQLLPGFRHVPFGNIEAMRTALNECKKTGDDVAAVILEP
                     IQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTGMGRTGKMFACEHENVQPDILC
                     LAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFGGNPLACAAALATINVLLEQNL
                     PAQADQKGDMLLDGFRQLAREYPDLVQEARGKGMLMAIEFVDNEIGYNFASEMFRQRV
                     LVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALAAMRVSVEEA"
     gene            complement(5917..6249)
                     /locus_tag="JNC24_003590"
     CDS             complement(5917..6249)
                     /locus_tag="JNC24_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HAY9160250.1"
                     /translation="METVAYADFARLEMRVGKIVEVKRHENADKLYIVQVDVGEKTLQ
                     TVTSLVPYYSEEELMGKTVVVLCNLQKAKMRGETSECMLLCAETDDGSESVLLTPERM
                     MPAGVRVV"
     gene            6468..7451
                     /gene="ebgR"
                     /locus_tag="JNC24_003591"
     CDS             6468..7451
                     /gene="ebgR"
                     /locus_tag="JNC24_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HAY9160251.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELTN
                     CYEHNGLPDIKNVTGILIVGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLFSVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
BASE COUNT         1825 a         2029 c         1868 g         1769 t
ORIGIN      
        1 tttcggtggc gttttttatc gcccacgcac taccagcgcc tggtccagct cgcgatacgc
       61 ttcaaccagt ttctctagtg aaacgcgact taaaccgctg ggatttggca gcacccaaat
      121 ctgcgtcgaa ccaatggtga gcgtttgttt cccccactgt gcaccgcgct ggctgaatcc
      181 ctgttcatat gcttgtttgc ccagaatcgc caacgcctgc ggctgataat cttcaatttt
      241 ttcaatcagc ttacgcccac ctgcgtgcag ctcctgcttt gaaacttcat tggcttgcac
      301 cgttggacgg tctaccagtt tggtgacgcc acaacgataa tccagcagat gctgtgcctc
      361 ctgcggcttc aactgacggt cggtaaaccc ggcctgatat atcaccttcc agaagcgatt
      421 tgctggatga gcaaagggaa aaccagtccc ggcggatgaa agcccagggt tgataccgca
      481 aaatacgacc cgtaaccctg gagccaaaat atcctcaacc atatttactc ctttctaaca
      541 tcaccaggaa aagtataacg gattgaaaat acattgttta taaaaacagc aggcgcacgg
      601 taatggctgg attgcgacac ggagttactt tataatccgc taccatggcc ccttagctca
      661 gtggttagag caggcgactc ataatcgctt ggtcgctggt tcaagtccag caggggccac
      721 cagatatagc aaaggctgac gagaaatcgt cagccttttt ctttttatat atcagttact
      781 ttgcgtgcca gtaagccgct gcacgtaccc gctgtgggtc atactgttcc gcttcaaagc
      841 ggcggcttaa attcttaacg actttacctt cgccggttat ccagatgaag taatcatcgg
      901 cagggatttg catctgcgcc agacgcgcat ctaccgcctg ctcatcatgt gccagccatt
      961 cgatattaaa accatcaagg tgcgcgagat aatcctgaca ggcgttatcc cgcacgctaa
     1021 ccagcgcact aacttgcggt ttaacggcaa gtttgctcaa cgtttccaag cggcggcgca
     1081 gtgcgggcat tccggattca tcgcagacat acagctgata cgcgtaatct tccggcacca
     1141 ccagcgaacc gcgcggacct gcaaccgtaa gtttatcgcc cggttgcgcc tgcatcgccc
     1201 agccgctggc gaccccaccg tcgtgaataa agaaatcgat cgccagttca tggcgtagtt
     1261 cgtcatacag cggcgtatag tcacgcgacg gtgggcgtgg tccttccggc cagacgatgc
     1321 cctcttccgt taccgttggc ggcacaaagt gagcgtcagg ttgaggaaag aagagtttgc
     1381 tgtgatcgtc aaagccgcgc gatgtaaaac cgtccagcgc ctcgccgccg aggacaatgc
     1441 gctgaaaacc ggcgctgatg cgctcaacgc gtaacacagt cagttcacgg aagcgcagat
     1501 cattgcgaac gcgctgcggg tagcgggggg agttattcat tgttatcgcc ttcgtgatgg
     1561 taatcagata tatctaaata attttcgtaa atgataatga ttgttaatca tgataaatgc
     1621 aagcgatttg tagaactgat atgtctatag tctgataaga cgaaccgcct cttctcaggc
     1681 atcattactc aacgccggat gcggcgtgaa cgccttatcc ggcctacgtg tgagatgagt
     1741 ccacatttaa aaatcatttt tattcttgca agagtgctca tatcagatat aaattagata
     1801 tatctaatca agcaaaagga ggctgatatg agccatcatc acgaagggtg ttgtaaacat
     1861 gaaggccagc cacgccatga gggctgctgc aaaggtgaga agtcagaaca cgagcactgc
     1921 ggacacggtc accagcatga acacggtcaa tgctgcggtg gtcgccacag tcgcggcggc
     1981 ggtcgtcggc aacgtttctt tggtcacggt gaattgcgtc tggtgattct ggatattctc
     2041 tcgcgcgatg acagccacgg ttacgaattg attaaagcga ttgagaatct aacccagggg
     2101 aattacaccc caagcccggg cgtcatctac ccaacgctgg attttctgca ggagcagtcg
     2161 ctgattacca tccgcgaaga ggaaggtggt aagaagcaga ttgcgctgac cgaacaaggc
     2221 gcgcagtggc tggaagaaaa ccgcgaacag gtggagatga ttgaagaacg catcaaagcg
     2281 cgttgcgttg gtgctgcgct gcgccagaac ccgcaaatga agcgggcgct ggataatttt
     2341 aaagcggtgc tggatttacg cgtcaaccag agcgatatca ctgatgcaca aataaaaaag
     2401 atcattgcgg tgatcgacca cgccgctttt gatattaccc aactggatta atcaccgatt
     2461 gccggatgcg gcgtacccta cacaccccgc aggcctgata agatgcgcca gcatcgcatc
     2521 aggcattgtg ctccaaccgc cggatccggc ataccgatta atgcagcacc gtcaccgcgt
     2581 cttccagtcg gctggcgcgg tgtttcacca tcgccgacac ctgcgcactc tcttccacca
     2641 gctcagcatt tttctgggtg atcaggttaa gctcatccac tgcacgggtc aggctggaaa
     2701 gcccatcggc ctgttccagc gttgaatggc taatctgggc gatcaactgg gtgacgtttt
     2761 tcacctgtgc cacaatatct tccatcgtcc gtccggcggc gtgtacctgc tgcgaaccgg
     2821 attgcacctt atcagcactg gcatcaatca gcttgcgaat atcgttggca gcattagcgc
     2881 tgcggctggc taaatgacgc acttcccctg ccaccactgc aaaacctttg ccctgttcgc
     2941 cggcacgcgc cgcttccacc gccgcattca gggccagaat attggtctga aacgcaatat
     3001 cgttaatcag cgaagtaatg gtgccaatgc gctgggtact gtcggcgata tcgtccattg
     3061 tcttgatcac cgtggtcatc gcctccccac cctgcactgc cgcattactg gcggtgatag
     3121 aaagtttatc ggcagccgac gccgtggcag agttctgttt caccgacgcc gccatttggt
     3181 tcatggtcgc caccgtttgc tgaacgttat caactgtctg ctgggtatgt tcgttcagtt
     3241 catcggtgcc tttcgccagc gtctcactgc catttctgac actggacacc tggcttgaga
     3301 catcgttaat tagccaacgg cacatcaggc caagctgccc taccgcgcgt aatgtcagcc
     3361 ccagctcatc gctgcgattc aggtgctcaa cactattacg ctctccggtc gccaccttca
     3421 gtgcctgacg ggcgacattt tctatcgggc gcacaatctg ccattcaaaa caagcgcttg
     3481 ccaacaatac cactaacaca cagaggaaat acgtcaccac cggggcagca acaaaccaaa
     3541 gcatggccgc cagcaagata aacatcaggg tcatcactcc acgcgcccgc cagcgaagcg
     3601 gtaatgaagg cagtttaccc agccagcctt tacgcaccac caggccttta tgaatacgct
     3661 tactggtacg tccggcgttc agcgctttgt acagcggctc caccgccgca atctcttcat
     3721 ccgtcgcccg ggtacgaatc gacatatagc cactgatttt tccctcgcgc accatcggta
     3781 ccgcattggc ccgcacccaa taatggtcgc catttttgcg gcgatttttc acgatgccgc
     3841 tccagggctc cccttttttc agggtgaacc acatatccgc aaacgccgct tttggcatat
     3901 ccgggtgacg caccatgttg tgcggctgcc cttgtaactc ttgcaaggta aagccgctca
     3961 cctgcacaaa agtgtcatta gcatgagtga tatagctttg cagatcggta gtggacatca
     4021 gagtggtatc gtccgccagc ggggtatttt gctgggtgac atacggatga gaagacatgt
     4081 tcgcgtcctg tgcaggttat atggttgtta acttcttgtc agagtttatg tcggccccgc
     4141 tgcggttatc tttaaccgat taatttgatt tagatcgcaa tttgcgatta aagcacaaat
     4201 ctaattcttt gatttaaaat actttcactc tgttactata cgaaaacgtt aattatcttg
     4261 cccaaaaatc aggcaattat tgccctgaaa acgtgcattt gcgcagcaat catcaaatcc
     4321 atacccgaca aaaaccgtgc aaaataacaa caaatgttaa cagatagcat taaatattgc
     4381 acaaatgata accgaatttg tgtttatccc gattttcgcg atcgcagccg gagtggcgca
     4441 atccctgcaa tacttaaatc ggtatcatgt gatacgcgag cctccggagc atattttgaa
     4501 caggttacct tcgagcgcat cggctttagc gtgcagcgcc cacgccctga atctcattga
     4561 gaagcgaacg ctggatcatg aggagatgaa agcacttaac cgagaggtga ttgaatactt
     4621 caaagagcat gtcaatccgg ggtttttaga gtatcgcaaa tctgttaccg ccggcgggga
     4681 ttacggagcc gtagagtggc aagcgggaag tttaaatacg cttgtcgaca cccagggaca
     4741 ggagtttatc gactgcctgg gaggttttgg aattttcaac gtggggcacc gtaatccagt
     4801 tgtggtttcc gccgtacaga atcaacttgc gaaacaaccg ctgcacagcc aggagctgct
     4861 cgatccgtta cgggcgatgt tggcgaaaac ccttgctgcg ctaacgcccg gtaaactgaa
     4921 atacagcttc ttctgtaata gcggcaccga gtccgttgaa gcagcgctga agctggcgaa
     4981 agcttaccag tcaccgcgcg gcaagtttac ttttattgcc accagcggcg cgttccacgg
     5041 taaatcactt ggcgcgctgt cggccacggc gaaatcgacc ttccgcaaac cgtttatgca
     5101 gttactgccg ggcttccgtc atgtgccgtt tggcaatatc gaagccatgc gcacggctct
     5161 taacgagtgc aaaaaaaccg gtgatgatgt ggctgcggtg atcctcgaac cgattcaggg
     5221 tgaaggtggc gtaattctgc cgccgccggg ctatctcacc gccgtacgta agctatgcga
     5281 tgagttcggc gcactgatga tcctcgatga agtacaaacg ggcatggggc gcacgggcaa
     5341 gatgttcgcc tgcgagcatg agaacgtaca gccggatatc ctctgccttg ccaaagcgct
     5401 cggcggcggc gtgatgccga ttggcgcgac catcgccact gaagaggtgt tttcagttct
     5461 gttcgacaac ccattcctgc ataccaccac ctttggcggc aacccgctgg cctgtgcggc
     5521 ggcgctggcg accatcaatg tgttgctgga gcagaactta ccggctcagg ctgatcaaaa
     5581 aggcgatatg ttgctggacg gtttccgtca actggcgcgg gaatatcccg atctggtaca
     5641 ggaagcgcgt ggtaaaggga tgttgatggc gattgagttt gttgataacg aaatcggcta
     5701 taactttgcc agcgagatgt tccgccagcg cgtactggtg gccggaacgc tcaataacgc
     5761 caaaacgatc cgcattgaac cgccactgac actgaccatt gaacagtgtg aactggtgat
     5821 caaagcggcg cgtaaggcgc tggcggccat gcgagtaagt gtcgaagaag cgtaatatca
     5881 aatcggatgg cgatacgacg tcgccatccg atctttttat acgacgcgta cgcccgcagg
     5941 catcatccgc tccggcgtta acagcacgct ttcactgccg tcatcggttt ccgcacacaa
     6001 cagcatgcat tccgacgttt caccacgcat tttcgctttt tgcagattgc ataagaccac
     6061 caccgttttt cccatcagct cctcttcgct gtagtacggc accaggctgg tcacggtttg
     6121 cagtgttttt tcccccacat caacctgtac gatgtacagc ttgtcggcgt tttcatggcg
     6181 tttcacttcc acaatctttc cgacgcgcat ttccagacgt gcaaaatcag cgtaagccac
     6241 ggtttccatt gcttcctccc ttcaagtaaa attttactaa actatagcaa aattttttct
     6301 caatcctgta ggctaaaaat ggagaatgca ggcgtgatca cattcgtaag ccgctgtgtt
     6361 accgttacag cgtcaaagaa acgcgcttta tttactgaaa acaggtgacc cgataagcac
     6421 ttcctctaca atgggggcgc acatcaggga aagtaaaaaa ggtaaacatg gcaacattaa
     6481 aagacatcgc aatcgaagct ggcgtatccc tggcgacagt atccagggtc ttaaatgacg
     6541 atccgacatt gaatgtgaaa gaagagacga aacatcgcat tctcgagatc gccgaaaagc
     6601 tggagtacaa gaccagtagc gcccgtaaac tacagacagg cgcagtcaac caacaccata
     6661 ttctggctat ctacagctac cagcaggagc tggagatcaa cgatccttac tatctggcga
     6721 tccgccacgg cattgaaact cagtgcgaaa agctggcgat cgaactgacc aactgttatg
     6781 aacacaacgg attaccagac attaaaaacg tcaccggtat tttaattgtc ggcaaaccca
     6841 cgcccgccct gcgcgccgct gccagcgcgt tgaccgacaa tatctgtttt atcgactttc
     6901 acgaacccgg cagcggttac gatgcggtgg atatcgatct ggcacgcatc agtaaagaaa
     6961 tcatcgactt ctatatcaac cagggcgtta atcgtattgg ttttattggc ggtgaagatg
     7021 agcctggcaa ggcggatatt cgtgaggtcg cctttgcgga atatggccga ctgaaacaag
     7081 tggtacgcga agaggatatc tggcgcggcg gtttttccag ttcgtcgggt tatgaactgg
     7141 caaaacaaat gctggcgcgg gaagactatc cgaaggcact gtttgttgct tccgattcca
     7201 ttgctatcgg cgtactgcgg gcaattcatg aacgtggcct gaacatccca caggatattt
     7261 cgcttttcag cgttaacgat atccccaccg cgcgatttac ctttccgccg ctctccaccg
     7321 tgcgcatcca ttccgagatg atgggaagtc agggcgttaa cctggtgtat gaaaaagccc
     7381 gcgatggtcg cgcgctgccg ctgctagtct tcgttcccag caaattaaaa ctgcgcggca
     7441 cgacccgtta aatcccctta cacactgtcc ggcaatcatt tttgccggac a
//
LOCUS       DADMKF010000181         7429 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.184, whole genome shotgun sequence.
ACCESSION   DADMKF010000181 DADMKF010000000
VERSION     DADMKF010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7429
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.184"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(<1..99)
                     /locus_tag="JNC24_003592"
     CDS             complement(<1..99)
                     /locus_tag="JNC24_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001433403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HAY9160252.1"
                     /translation="MTLTRREFIKHSGIAAGAQVVTSAAPLPAWAEE"
     gene            complement(124..1224)
                     /locus_tag="JNC24_003593"
     CDS             complement(124..1224)
                     /locus_tag="JNC24_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C"
                     /protein_id="HAY9160253.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKSFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFETHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDPGLADRKPLWQYAEKLDDTYCAGCHAPIAADHYTVNAWPSIA
                     KGMGARTSMSENELDILTRYFQYNAKDITEKQ"
     gene            complement(1612..2358)
                     /gene="cutC"
                     /locus_tag="JNC24_003594"
     CDS             complement(1612..2358)
                     /gene="cutC"
                     /locus_tag="JNC24_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HAY9160254.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLYTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DEHADEYSRYIVDGAAVAEMKGIIERHQAK"
     gene            complement(2372..2938)
                     /locus_tag="JNC24_003595"
     CDS             complement(2372..2938)
                     /locus_tag="JNC24_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAY9160255.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSLRLGLNITPLTADHIS
                     LRCHQNATAERWRRGFEQCGELLSENMINGRPICLFKLHEPVQVAHWQFSIVELPWPG
                     EKRYPHEGWEHIEIVLLGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            3154..4890
                     /gene="argS"
                     /locus_tag="JNC24_003596"
     CDS             3154..4890
                     /gene="argS"
                     /locus_tag="JNC24_003596"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HAY9160256.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQVRHSAKVQFGDYQANGM
                     MAVAKKLGMTPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVSTPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGDEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKL
                     QSGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAK
                     GLAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRV
                     LYYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLA
                     DLLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNM
                     LAFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETL
                     TVVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLG
                     LDTLGIETVERM"
     gene            5067..5429
                     /locus_tag="JNC24_003597"
                     /pseudo
     CDS             5067..5429
                     /locus_tag="JNC24_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416391.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
     gene            complement(5433..6503)
                     /locus_tag="JNC24_003598"
                     /pseudo
     CDS             complement(5433..6503)
                     /locus_tag="JNC24_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416393.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            complement(6496..>7429)
                     /gene="flhB"
                     /locus_tag="JNC24_003599"
     CDS             complement(6496..>7429)
                     /gene="flhB"
                     /locus_tag="JNC24_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhB"
                     /protein_id="HAY9160257.1"
                     /translation="DPNLILGQIILLIREAMLALLPLISGVVLVALISPVMLGGLVFS
                     GKSLQPKFSKLNPLPGIKRMFSAQTGAELLKAILKTILVGSVTGFYLWHHWPQMMRLM
                     AESPTTAMGNAMDLVGLCALLVVLGVIPMVGFDVFFQIFSHLKKLRMSRQDIRDEFKQ
                     SEGDPHVKGRIRQMQRAAARRRMMADVPKADVIVNNPTHYSVALQYDENKMSAPKVVA
                     KGAGLVALRIREIGAENNVPTLEAPPLARALYRHAEIGQQIPGQLYAAVAEVLAWVWQ
                     LKRWRLAGGQRPVQPTHLPVPEALDFINEKPTHE"
BASE COUNT         1920 a         1947 c         1776 g         1786 t
ORIGIN      
        1 ctcttctgcc catgcgggga gcggtgctgc cgatgtcacc acctgcgctc cagcggcaat
       61 accgctgtgt ttaataaatt cacgtcttgt taatgtcata acttcctccc tgatcaacga
      121 ggatcactgt ttctcggtaa tatctttggc gttgtactgg aaataccgcg ttaaaatgtc
      181 cagttcgttt tcgctcatgc tggttcgtgc ccccattcct ttggcaatgg acggccacgc
      241 attgacggtg taatggtcgg cggcaatagg ggcatgacaa ccagcgcaat aggtatcgtc
      301 aagtttttca gcgtattgcc atagcggttt acggtccgct aatccgggat cggtaagcgc
      361 accctgtaaa gacgcctgac gccattgatt gccgtattcg tcagcctgcc attccccgtt
      421 taccgtgagc gccttgatac cttcttcact taatgtggct agcgccagcc gttgacctgc
      481 cgccaggtag agcgtgtttt cactgccctg catttgataa ccctgcaaca gaacgatcgg
      541 ctgtttgcca ctggcatcaa cgacggtgag atcggtacca ggattcacgg tagccagctc
      601 gcctatgcga gaagttttga aaggataaat atgtgcgcca ttagtcactg aagtagcggc
      661 ctgactttcc agctcatgcg ccgcgttgtc atccattttt atttctggcg gaaaatgggc
      721 aatgccttta tgacaatcga tacaggtttc gccgcccttt tgtgctttgt tatgcatttt
      781 ctgcgcagat tcactttgcg aggcaatatc catggcatca aaagaatggc aactacggca
      841 cgttgcagag tcagttgctt ttaattcttt ccatactgtt tcggccattt cctggcgatg
      901 agtttcgaac ttatcgtcac tgtctatttt gccgctaaca aattcatgat aaatatcttt
      961 agatgctttt aatttagcaa ataaataatc catccctgac tttggaatat ggcaatctgc
     1021 acattccgca cgtatccctt tctggttcga aaagtggaca gttccctgat attcctcaaa
     1081 agatttactc atcgagtggc aggaaaggca aaatgctgta tccgacgttt tatgtaaggc
     1141 tttttgcgcc agcaataacc cgccgccacc aaccacaacc gctatcagca aaaacaataa
     1201 cccaatgcgt tttttccctc gcatagattc ctctaaataa attccagaac accggaatca
     1261 ttgtataatt aaatacacaa cgaattaacc gcttgcataa ctcggcacaa cactgcctga
     1321 aataatcgat aaagaatatg atttattaca atgtaatcat taattgctaa ggaataaccc
     1381 agttgctatt tagaatatat atttatcagt tttagcaatt taaatcccat aatcaatgtg
     1441 aatataaaaa atattattag cacaccgcta tgttcactgt cacccgctca acagatcacg
     1501 gttatcgttc gttttttata ctgctcaggg ataaaaaaag gcccctgttg aaattgcagg
     1561 ggcctggtac gagcaagcat catattgggc gacatgatgc aacggtaaaa atcatttggc
     1621 ctgatggcgt tcaatgattc ctttcatttc agcaaccgcc gccccgtcta cgatataacg
     1681 cgaatactcg tccgcgtgtt catctgatga catggacaat ccttgattac gataacgcat
     1741 cggtgaggct tgccacgctc ccgcggagct atggacttcc agcactccgg catcgaggaa
     1801 gtggtgcagg ttttctgcac ggactcctgc tccggccata atgattggag catcacgatg
     1861 ggcaataagt tccataattt ttgataaacc ttgcagtgcg tctgattttt gccctgatgt
     1921 cagtacccgg gcaatgccca attccgcaag attattgagt gtatataaag ggttagcgca
     1981 catatcgaag gcgcgatgaa aagtcactgc cagcggaccg gcagcagcca ttattttttc
     2041 cattcgtggc atatcgacat tcccgtcaac atcgagaacg cccgtcacca gtccaggaaa
     2101 acctaattcg cggaccgtgc gcacatcctc aagaatggcg gcaaactcac cgtcgctgta
     2161 gcaaaaatca ccaccgcgtg ggcgaattat cggatgcaca gggatcgtca cccgctggcg
     2221 cacggatttc agtaccccca acgacggcgt taagcccccc tcttttgggg ctgcgcataa
     2281 ttcaactctg tctgcgccgt tttgctgcgc cgttagtgca cattccatgc tgtaacagca
     2341 aatttccagt aatgccattt ttgctcctta attacgctga ctgctcgctg gcaacgatct
     2401 cttcaatgga ccacggatga aacttaatag ttgttttgcc atcggtgacc gccagcgtcg
     2461 gattcggtaa tcgctcatgc tcaccttttg gtgaactggt tttaactgag atccccggta
     2521 ggctgagtcc ttcatcagaa agcaatgcca gcgcacgggc attcagcgtt tctggatcac
     2581 ccagcagaac gatttcaata tgttcccaac cttcgtgtgg gtaacgtttt tccccgggcc
     2641 acggtagctc cacaatagaa aactgccagt gcgcaacctg taccggttca tgcaatttaa
     2701 acagacaaat cggtctgcca ttgatcatat tttctgacaa aagctcgcca cactgttcaa
     2761 acccgcgacg ccagcgttca gcagtggcgt tttgatggca acgcaaagaa atgtgatcgg
     2821 cagtcagcgg agtgatattc aaccccagac ggagggaaag ttcatctaat gcgtgggtaa
     2881 atcgcggtaa atccgatgca atatcctgca gctcgtcgat agattgccag ttcgccataa
     2941 tcactcttcg tctttcagta aaagcgttaa tttaccctgt tgccctgcgc caaccaaccg
     3001 ctgatttcac gccgcttctg atgcaatagt gaaaacggca atacgccacg cgcacgttgc
     3061 tgacgaaaac agccatttgc agtatactcc cgccctaatt tctttaactg gtgcgggcaa
     3121 tttttgctcg cttcatcaat gtaaggtatt ccggtgaata ttcaggctct tctctcagaa
     3181 aaagtccgtc aggccatgat tgcggcaggc gcgcctgcgg attgcgaacc gcaggttcgt
     3241 cattcagcaa aagttcagtt cggcgactat caggctaacg gcatgatggc agttgctaaa
     3301 aaactgggta tgacaccgcg acaattagca gagcaggtgc tgactcatct ggatcttaac
     3361 ggtatcgcca gcaaagttga gatcgccggt ccaggcttta tcaacatttt ccttgatccg
     3421 gcattcctgg ctgaacatgt tcagcaggcg ctggcgtccg atcgtctcgg tgtttctacg
     3481 ccagaaaaac agaccattgt ggttgactac tctgcgccaa acgtggcgaa agagatgcat
     3541 gtcggtcacc tgcgctctac cattattggt gacgcagcag tgcgtactct ggagttcctc
     3601 ggtcacaaag tgattcgcgc aaaccacgtc ggcgactggg gcactcagtt cggtatgctg
     3661 attgcatggc tggaaaagca gcagcaggaa aacgccggtg atgaaatgga gctggctgac
     3721 cttgaaggtt tctaccgcga tgcgaaaaag cattacgacg aagatgaaga gtttgccgag
     3781 cgtgcacgta actacgtggt aaaactgcaa agcggtgacg aatatttccg cgagatgtgg
     3841 cgcaaactgg tcgacatcac catgacgcag aaccagatca cctacgatcg tctcaacgtg
     3901 acgctgaccc gtgatgacgt gatgggcgaa agcctctaca acccaatgct gccaggaatt
     3961 gtggcggatc tcaaagccaa aggtctggca gtagaaagcg aaggggcgac cgtcgtattc
     4021 cttgatgagt ttaaaaacaa ggaaggcgaa ccgatgggcg tgatcattca gaagaaagat
     4081 ggcggctatc tctacaccac cactgatatc gcctgtgcga aatatcgtta tgaaacactg
     4141 catgccgatc gcgtgctgta ttacatcgac tcccgtcagc atcaacacct gatgcaggca
     4201 tgggcgatcg tccgtaaagc aggctatgta ccggaatccg taccgctgga acaccacatg
     4261 ttcggcatga tgctgggtaa agacggcaaa ccgttcaaaa cccgcgcggg tggtacagtg
     4321 aaactggccg atctgctgga tgaagccctg gaacgtgcac gccgtctggt ggcagaaaag
     4381 aacccggata tgccagctga cgagctggaa aaactggcta acgcggttgg tattggtgcg
     4441 gtgaaatatg cggatctctc caaaaaccgc accacggact acatcttcga ctgggacaac
     4501 atgctggcgt ttgagggtaa taccgcgcca tacatgcagt atgcttacac gcgtgtattg
     4561 tccgtgttcc gtaaggcaga aattaacgaa gagcaactgg ctgcagctcc ggtaatcatc
     4621 cgtgaagatc gtgaagcgca actggcagct cgcctgctgc agtttgaaga aaccctcacc
     4681 gtggttgccc gtgaaggcac gccgcatgtg atgtgtgctt acctgtacga tctggctggt
     4741 ctgttctctg gcttctacga gcactgcccg atcctcagcg cagaaaacga agaagtgcgt
     4801 aacagccgtc tgaaactggc acaactgaca gcgaagacgc taaagctggg tctggatacg
     4861 ctgggtattg agacagtaga gcgtatgtaa tcgatttttc gtgagagtaa agcctgatca
     4921 gggttacctg atcaggcttt tttattgcca tctaaatgta ttctgaaaat ggacatgcca
     4981 ttgttttctg actgttggat aagaggccag aagcgtaata tccggcccca gggaaacgat
     5041 aacggttgaa tttaaggaat accgcagtgt ttaaatttct tgtattaaca ttaggcatta
     5101 tctcttgcca ggcttacgca gaagatacgg ttatagaaaa cgaccatgac atttcagcca
     5161 tcaaagattg ttggcaaaaa aattcagatg atgatactga cattaacgtg atcaaatcat
     5221 gcctgcgaca agaatacaat ctcgtcgatg cgcaattaaa taaagcctat ggtgaagctt
     5281 atcgttatat agaacaagtg ccacgcacag gtgtaaaaaa acctgatacc gaacaactta
     5341 acttgcttaa aaaatcacag cgagcctggc tggattttag ggacaaagaa tgtgaattaa
     5401 tcctttcaaa tgaggacgtt caggatttaa tgtacatcgt tccacgtcgc ttcgacaacg
     5461 gtattattct ctgccatttt taccggtttg ggttcggcag gcgctttttg ttcgcgtccg
     5521 cgtatccacc aggccagccc gagcaatccg gcagtgaaca gcaaaaatac caggttcggc
     5581 attccaggca ccaggccgag taaaccgagc acggcggcgc ttaacaacat aacgcttggg
     5641 ttactgaaaa gctgattcac catctgctcg ccaacatcct gatcggtgct gacacgcgta
     5701 acgatgaccc ccgcggcggt aaaaatcacc agcgccggaa tttgtgccac cagaccatcg
     5761 ccaatggtca atagcgtata actttccgcc gcgtgtccca tgctcatgcc atgttgcagc
     5821 acgccgacca gcaacccgcc gacaacgtta atgaccatga tgaggatccc ggcgatggca
     5881 tcgccgcgaa caaacttact tgccccgtcc attgagccgt aaaaatcggc ttcctgagtc
     5941 acttcggagc ggcgtttttt cgcctcatct tcaccaatca atccagcgtt aaggtcggcg
     6001 tcaatcgcca tctgcttacc cggcatacca tcgagaacaa agcgcgcacc cacttctgcg
     6061 atacgcccgg cacctttggt aatgaccata aagttgatga tcacgagaat gacaaacacc
     6121 acgataccga tagcgaaatt gccaccaacg aggaagtgac cgaacgcttc gaccaccttc
     6181 cctgccgccg ccgcgccggt atgcccttcc atcaaaatga tacgggttga agccacgtta
     6241 agtgccagac gcaacaacgt agtaaacaac agaatggtcg gaaacgcagc aaactcaagc
     6301 gtgcgctggg taaacatcgc caccagcaac accatgatcg acaaggcaat attgaaggta
     6361 aacaacaggt cgagtatgaa tgcgggcagt ggcagcacca tcatcgacaa gatcaacagg
     6421 atcaaaatcg gtccggcaag gatctgccat tgtgtcgatt tcaggtttgc gggcaggcgc
     6481 agcatcgcgg ccagattact catgggtcgg tttctcgtta ataaaatcca gggcttccgg
     6541 caccggaaga tgagtaggtt gtacagggcg ctgtccacca gccagacgcc agcgtttcag
     6601 ttgccagacc caggccagca cttccgccac cgcggcgtac agttgacccg ggatttgttg
     6661 accaatctcc gcatgtcgat acagcgctcg cgccagcggc ggcgcttcaa gcgtcgggac
     6721 gttattttca gcgccaattt cacgaatgcg cagcgcgacc agccctgcac ctttagcgac
     6781 cactttcggt gcgctcattt tgttttcgtc atactgcaac gctaccgaat agtgggtcgg
     6841 gttattgaca atgacatcag ctttcggcac atcggccatc atccgacgcc gtgcagcagc
     6901 tcgctgcatc tgacggatcc gccctttaac atgagggtca ccttcgcttt gtttgaactc
     6961 atcacgaata tcctgccgcg acatacgcag ctttttcagg tggctgaaga tttggaaaaa
     7021 gacgtcaaat cccaccattg gaatgacacc aagcaccacc agcagtgcgc atagcccaac
     7081 cagatccatc gcattaccca tggcggtagt cggagactcg gccattaagc gcatcatctg
     7141 cggccagtga tgccagagat aaaaccccgt cacgctgcca accaggatgg ttttcaaaat
     7201 tgccttaagc aactccgcgc cagtctgtgc cgagaacatc cgtttaatgc ccggtagcgg
     7261 gttgagtttg gaaaacttcg gctgcaagga tttgccgcta aataccagcc cccccagcat
     7321 gaccggagaa atgagcgcca ccagcaccac gccgctaatc agcggcagca gcgccagcat
     7381 ggcttctctg atcagcagaa taatctgccc gaggatcaga ttcgggtca
//
LOCUS       DADMKF010000182         7268 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.185, whole genome shotgun sequence.
ACCESSION   DADMKF010000182 DADMKF010000000
VERSION     DADMKF010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7268
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.185"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(1..4518)
                     /locus_tag="JNC24_003600"
                     /pseudo
     CDS             complement(1..4518)
                     /locus_tag="JNC24_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616597.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Rhs"
     gene            complement(4538..4999)
                     /locus_tag="JNC24_003601"
     CDS             complement(4538..4999)
                     /locus_tag="JNC24_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
                     /protein_id="HAY9160258.1"
                     /translation="MQFTFNEGHIQLPSQWQDQSMQVLVSTDNSGINLVITREAVPQG
                     TLTPELYQETLALYQGKLDGYTEHACREITLAEAPAWLLDYSWNGPEDEGNQGRISQI
                     AVFQRRGDTLLTFTFSTSLSLKNSQKTMLLEVIKSFTPLPPENDIQKDQPR"
     gene            complement(5027..6928)
                     /locus_tag="JNC24_003602"
     CDS             complement(5027..6928)
                     /locus_tag="JNC24_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HAY9160259.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTIWQGDEVQRRVKGVVTWFELGENDKNQMLYSMKVHPPLWRA
                     GLRQNFRIFQNEDIKSILGTMLQENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAYKSTDQSLVLCDTVRHLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWAGRFEQEGQHQDYQRTQYEVYDYPGRFKGAHGQNFARWQ
                     MDGWRNNAETARGMSRSPEIWPGRRIVLTGHPQANLNREWQVVASELHGEQPQAVPGR
                     QGAGTALENHFAVIPADRTWRPQPLLKPLVDGPQSAVVTGPAGEEIFCDEHGRVRVKF
                     NWDRYNPADQDSSCWIRVAQAWAGTGFGHLAIPRVGQEVIVDFLNGDPDQPIIMGRTY
                     HQENRPPGSLPGTKTQMTIRSKTYMGSGFNELKFDDATGREQVYIHAQKNMDTEVLND
                     RTTTVKHDHRETVKNDQTVTIQEGNRLLTVEKGHKITGVLKGSLSEDVFQDRGTIAGS
                     VHVDAVNNGGEGDGIQAYTAIKEILLAVEESKIALTPDSIQLQVGESTVIRLSKDGIT
                     IVGGSVFIN"
BASE COUNT         1379 a         2206 c         1960 g         1723 t
ORIGIN      
        1 ttatttgact acgtgataaa cgaaccccat ccaggaaacc cgtatgccca ctaacacgac
       61 gaccatcgcc aatatagtcc atcaaactgc tgattttaga acttgcagcc aatcccagcg
      121 gatcaaccca cccacaagga ttcggcacat accggtaagt ctgctccccg ccctgcaacc
      181 ccagcggatc actcaccagg tacattccgg tttccggctc gtaataccgg aaccgattat
      241 agcacagccc ggattccgca tcctgccact gtcctgcata cagcaggccg gggtcggctt
      301 ccgggtccag ttccccgcgc gggtccgggt agccggtgtc tgcgggcagg ctgagtgcca
      361 gcccccacag gctggtctgc cccggtcgcc agaccgtctt tccttcactg ttaaagagca
      421 tcagcgggcg gcccgtcagg tcgctcaccg catgattcgt ccgcgtcacc cactgcgggg
      481 ccaccgatgg atgcgcctgc cgtacccggc tgaactgctg gcttatcagc tcaaagccgt
      541 taaacaccag gtgccttacg ctgtacagtt tatcatcgcg gtattcccgg atttccgcaa
      601 tactgtcgcc gtcccacaga tacgtgaagc ggattttctt ccggtcgcag cgtttctctg
      661 ttcgtctgcc gctggcgtcg tggcggtatt cccacagctc accctgtgcg ctgcaatacc
      721 cggtcagctg gtcccggctg tcccaccgga accgctctgt ttccggtcgg tagccgtcac
      781 ggtgtctggt gcggctgacc atccggcctg cggcgtcata gtcatactgc gtgtttccgg
      841 cctgtttcag ccggtggcct ccggcatact ggtcagtctc ttcacttatc cggtgcctgc
      901 ccgcagactg cgccttcagg tagccgcagc tgtcatagcc ataggactca tcgccttcgc
      961 catagcccag cccggtgcct gaggctgtca ccgccgtgac ctggccgttc cggtccagcc
     1021 gatactcccg ccgtgtttcc cgtccgtaat cttcccggct ggtgacgcct gacacgttac
     1081 ccgcgctgtc gtaacggtac tcccgtacca gtgagggaac cagcccgtcc catgtggctt
     1141 ctgccggaaa aaatccggcg cgctgactga caggctgacc ggctgcattc cagccctgtt
     1201 cacaggcaaa accccggtta ctgtgccgga gcacttcacg tcccgcctca tcacggctga
     1261 acgtcagcgt ctcatccccc ggcagaatga cctctgtcag ttctccggcg gcgttgtagc
     1321 ggtacgtggt tttcagcagg gattcaccgg ggtgtctgtc gcgttcatct tccggtggca
     1381 ggatacagcg gatgacggtg tgttcatcgg ggtagttgta ttcaatggcc cgtcggccct
     1441 gagtggtgac attcacccgc ccgcgttcgt catagccggt gcgctgtgat gtgccgtccg
     1501 ggctgaagat acccgtcagc ctgccggatt tgtcataggc atactgcgtt ttgccttccg
     1561 gcgagatgat ttctgccagg cggcctgcgg cgtcccggct gtagaggatg gtttcctcca
     1621 ggccgttaac cttggcggta cagaggccat cggcgtcata gcggtaacgc cagaccacgc
     1681 cgccccagtc acgctcttcc gtcacccggc cgcagttatc cagaacataa agatatgact
     1741 caccctgcgg gttgatgact tctgtcagtt gcagcgtgtc tttatcgtag cggtactgcc
     1801 agcggtggcc ttcaccgtcc gttcgcgcca ccggaaaatc gaacggaccg tattccatcg
     1861 tccagacaac accgttgccg tccgtccagg ccagcaggtt gccgtggcgg tcatagcgaa
     1921 atccttccgc gccggttcca gcacgttcaa tgccgctcag tttatggtgc tgctgccact
     1981 gtaaccgcac tgactgaccg ccggggccgc gcatcttcac tggcaggtcg tgaccgctgt
     2041 attcggtgtg gtgttcacgg cccatcacgt cggtgagtgt ttccggccgg ttgtggtggt
     2101 cgtactctat gcccagacgc cgttcatcct ggcggaatat tcccgacagc agcccctgag
     2161 cggtgtatgc cagccgggtc tcttcgccct gtggcgacac cagacttacc ggcaggccgc
     2221 gttcatcaca ctgctgtgtc cacgtctgcc cggcagggtc ggtgatgttg ctgagcaggc
     2281 cgcgctcatc atagcggtac tgccactccg cgccgtctgc cgccgtgtaa cggcttaact
     2341 gaccgtgtgc ggcgttgtat tcaaatttct ccacccgccc ggcaggggag tggcgggcca
     2401 gcaggtgatg gaattcatcc cattcataca tggtggtgct gccgtccggc gcttcttccc
     2461 gcagaatatt gccgtcagga tcatagtaat aacggtgaat gcccttgccg tccgtcacgg
     2521 tggtgcagcc gtcaccatag tccagcgtgg cgttgtaata gccctccgcc ccggtgacat
     2581 tcacgcaccg gccctgttca tcgtaggtga aacggctcca cgtctggtcg ttatcggacc
     2641 agcggatgat ccggtccgca gcgtcgtact cataaaacag gtggtagtcc agccgcgcat
     2701 ccgcttctgt cagccgcccc cgcgcatcct gttcgtaggt cgccagcgtc tgtatgtcgt
     2761 tatccgtgcg gtgaatggct ttcaggtagc cgctggcgtg gtacgtcagt ttaagccgga
     2821 tcccgtcggt gtgactgaca cccgtgatcc ggtactggct gtcgcgctca aacgtgatgc
     2881 ggttgctggc ggtgtcgctc atcccgccca gcatgtacat ccggtcattg gggctgtgtg
     2941 ccaggcggtc atcgctggta tcgtcagtca ccgggtggcg cagacgcatt cccggaaagg
     3001 cgtcataaaa gaagcggctg ctcagtgcat ccctgtcgaa cagctccaga taatcatcgc
     3061 ggcggcgcag aatgtactgg gcgtgatacg ggttaaccgc cgtatcactg tcaccgtcga
     3121 aggcaaatct cagcgtggct ccctggctga tgatatccac ccggtttccc tgttcacgca
     3181 cccaggcgta ttcgctccag gagtcgctcc agcccacgcc ggtcaggcca tgcagcggca
     3241 gtacagaact gtggaaacgg gtgaaggaga gggggagggt ctggcccagg gtgaagtcgg
     3301 tacgttcgtc gcagtatgcg ccggttaccg gatcgacagg atctgttacc catctgatga
     3361 ctttattggc aatacttttt aacgacttac ctgttttccc cagcaacgcg ccgagctttg
     3421 cgcccagcag cttcccgctt ttccccagca gtttcccagt ttgccgagtc tcgccagatt
     3481 cttcagtttt cccagtagtc ccgctggcgg cagaagatag ctggctatcg ttattccccc
     3541 caggatcatt ctctgccacg gcgggaactc cggctggata tccagatatg tgacctgctc
     3601 accgccaata aagacgtccg gcgaacctcc cacgatcgcc gcactgcact ccagtttgtc
     3661 gcccttgcgc gcggcaggct gattgttgat aaagacgttg ctgctgccct gggctatcct
     3721 cggtggtttg ttctccttgt cgcagagcgc cgtgtcctca ccggcttccg ggaaatccac
     3781 atcttttttg atttcgccac gcccgcccac catggtggca aactccatta ataccggacc
     3841 ggcgggaaac agaagcgatg cgccggcgct gacgcgatcc ataaaaccgg cattgctgtc
     3901 cgtcagggtg ataacagcgt tcccccaggc cttcgccagt ggtactgctt tttccgctgc
     3961 cgattcaagc aggtcagtgg caatatcacc ccatgagggt tccccctgtg ggacttcagg
     4021 tattgctcca ggtgggggag gagggggaag ggtgaccgct gcacgtgcgg ccagctcgcc
     4081 gttaattttt acgttttcag aaccggtatt gatggcgccg tccgtgtcac aaaagctgtt
     4141 tatccatgat tcaagctggt tctgtttctc ctccacataa tcactcgcct tatagctggc
     4201 atacatcagc gcaggtacca gaaccagcgc ataagggccg gtggcacagg cggccataat
     4261 caggcaacct tctgcgatgg tcactatcgc ccccagtacg gcccctgcca gcgcccccaa
     4321 aaaactcttg tgctgaattt catcgcccag cctggcggcg ggggggccag acttttttga
     4381 tgccaacatc tgtgcaatgg ctttgtcgtt ggctgcccgt ttcatctggc tggcgaccat
     4441 cgcctgctgt gacagcattc gcattgccag cgtaccgcct gcggtcgctc cctgtggccc
     4501 gcctggtcct tcactcatgt gttatccctc gtttatgtca gcgcggctgg tccttttgga
     4561 tgtcattctc cggtggcaac ggggtgaagc tctttatcac ctccagcagc atcgtctttt
     4621 gactgttctt cagggataag gaggtgctga aagtgaaggt cagcagcgta tctccacgcc
     4681 gctggaatac ggctatctgg ctaatccgcc cctgattccc ctcatcttcc ggaccgttcc
     4741 aggaataatc cagcagccag gccggggcct ctgccagagt gatttcccgg caggcatgtt
     4801 cggtgtaccc atcaagtttg ccctggtaca gcgccagcgt ttcctggtac agctctggtg
     4861 tcagggttcc ctgtggaacg gcttcgcggg taatgaccag gttgatgcca ctgttatctg
     4921 tggacaccag gacctgcatt gactgatcct gccattgaga cggcagttga atgtggcctt
     4981 cattaaaagt gaactgcata aaatactccc tgataatctg agtgaatcag ttgatgaaaa
     5041 cagaaccgcc cacgatggtg atgccatcct tcgacagcct gattaccgtc gattccccga
     5101 cctgtagctg aatgctatcc ggcgtcagcg caattttgct ttcctccacg gccagcaaaa
     5161 tttccttaat cgccgtataa gcctgtatac cgtcgccttc gccaccattg tttacagcgt
     5221 caacatgcac cgaaccggca atcgtgcctc tgtcctgaaa gacatcctca gataaagacc
     5281 ctttcagtac tccggtgatc ttgtggcctt tttccaccgt aagaaggcgg ttaccttcct
     5341 ggatcgtgac cgtctggtca ttttttacgg tttcgcggtg atcgtgtttt acggtggtgg
     5401 tacggtcgtt gagcacttcg gtatccatgt tcttctgcgc gtggatgtag acctgttctc
     5461 tccctgtcgc atcatcaaac ttcagctcat taaatccgct gcccatgtac gttttggagc
     5521 ggatggtcat ctgcgtcttt gttcccggca ggctgccggg ggggcggttt tcctggtggt
     5581 aggtgcgccc cataatgatc ggctggtccg gatcgccgtt gaggaagtct acaatcactt
     5641 cctgacccac acgcggtatc gccaggtggc caaaaccggt gcctgcccac gcctgtgcca
     5701 cacggatcca gcacgaactg tcttggtctg ccgggttata acggtcccag ttgaacttca
     5761 cccgcacgcg accatgttcg tcgcagaaga tttcctcgcc tgccggtcct gtcacgacag
     5821 cgctctgcgg gccgtcgacc agcggtttca gcaacggctg tggtcgccat gttctgtctg
     5881 ccgggatcac cgcaaaatgg ttctccagcg ccgtccccgc tccctgccgt cctggcaccg
     5941 cctgtggctg ttcgccgtgc agttcacttg ccaccacctg ccattcccgg ttcaggttcg
     6001 cctgcggatg ccccgtcagc acaattcgtc gtcccggcca tatctccggc gagcgactca
     6061 ttccccgcgc ggtttctgca ttgtttcgcc agccgtccat ctgccagcgg gcaaagttct
     6121 gcccgtgagc acccttgaaa cgtccggggt agtcatacac ttcatactgc gtgcgctggt
     6181 agtcctggtg ctggccttcc tgttcaaaac gtccggccca gccggggcgt ttaaaggtgt
     6241 agtctttggt caccacggaa gaagggcgga tttgtgcgct gtagcggaac tggctgatgc
     6301 agagggtgct cacctcggta cgggtgttcg ggttccatgg gatttcaaaa gattcgggca
     6361 gatggcggac tgtgtcgcac agcaccaggc tctggtcggt acttttgtaa gcatgctcct
     6421 cataaaagaa gatgccttcc tccgccgcca tccggcacag gaaatcgtaa tcagtctcac
     6481 cgtactggac acaaaactca cgggaaggat gcggctcgct gaacagcgga ctccattcgg
     6541 tcaccccgtt ttcctgcaac atcgtgccga ggatgctttt gatgtcctcg ttctggaaga
     6601 tacggaagtt ctggcgcaga ccggcacgcc acagcggcgg gtgcaccttc atgctgtaca
     6661 gcatctggtt tttgtcgttc tccccgagtt caaaccaggt caccacgcct ttcacccggc
     6721 gctgtacttc atcgccctgc catatcgtca ggtaggccat tttgtccagc acctgcgcaa
     6781 attcaaggga gagaaactgc tggctgacca gggagagatc gagggaaaag agcgaagaga
     6841 gtgactggtt cagatgaaag gaaaccaccg caaaagcgtc cggcggcagg ccgtccactt
     6901 ccagtgtgaa acgtaatccg gttgacatag caacctccag taaaatgaat aatgatccgt
     6961 accgtacaac tgcggataat gtgataagcg ggacgacttc agacgttatt agggaaggaa
     7021 cagaaaatgc tatggttatg tgagaatttt gtttaaatat tactttacag aaacgggtgt
     7081 ctgttcagtc aaaaaaataa tcacaagctc aacctcttgt ttcttggcgt tattcaggac
     7141 aggtacgcat atcacaacga gtaacaaaaa cgcaaagtta aaccaatatt cataatatat
     7201 ttttattcac agcaataaat aatccaatta acattattaa aatgaatgat tgcataagaa
     7261 atatatgc
//
LOCUS       DADMKF010000183         7239 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.186, whole genome shotgun sequence.
ACCESSION   DADMKF010000183 DADMKF010000000
VERSION     DADMKF010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7239
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.186"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            <1..337
                     /locus_tag="JNC24_003603"
     CDS             <1..337
                     /locus_tag="JNC24_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAY9160260.1"
                     /translation="ADLSRYSGTVVNRPTFFAFAKGAGVMGEAGPEAILPLRRGADGK
                     LGGVADTGGSGMVMFAPQYNIEINNDGTNGQIGPAALKVVYDLGKKAAADFMQQQARD
                     GGRLSGAYR"
     gene            337..666
                     /locus_tag="JNC24_003604"
     CDS             337..666
                     /locus_tag="JNC24_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY9160261.1"
                     /translation="METFHWKVRPDMNVVSEPKVVTVKLGDGYEQRRAAGLNNQLSTY
                     SVKIRVRKGEHPFLKAFLERHGGVRAFQWTPPYDWKPIRVVCRKWSASVGALWVTITA
                     DFEQVVA"
     gene            676..1374
                     /locus_tag="JNC24_003605"
     CDS             676..1374
                     /locus_tag="JNC24_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HAY9160262.1"
                     /translation="MQDIPQETHHETTRLTQSAQVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQAYPIQGTGFELNGKGSAARPTLTVSNLHGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLATPTETDG
                     AVFPGRIMLANTCMWTYRSDECGYTGRAVADEFDKPTTDIRKDKCSKCMRGCELRNNT
                     GNFGGFLSINKLSQ"
     gene            1379..2122
                     /locus_tag="JNC24_003606"
     CDS             1379..2122
                     /locus_tag="JNC24_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY9160263.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVVRTPEGDRYLPSENISGEPE
                     ERFRMAPEDWLRAQMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGAIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHRGDDWWRNGQNLYLDNMEVT
                     GFYRVALTEAQPGDVLLCCFGSSVPNHAAIYCGDGGLLHHIPEQLSKRERYTDKWQRR
                     THSLWRHRAWRASAFTGIYNDLVAASTFV"
     gene            2059..2661
                     /locus_tag="JNC24_003607"
     CDS             2059..2661
                     /locus_tag="JNC24_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HAY9160264.1"
                     /translation="MARICLYGDLQRFGRRIDLRVKTGAEAIRALATQLPAFRQKLSD
                     GWYQVRIAGRDAGETELSARLNEPLANGAVIHIVPRLVGAKSGGVFQAVLGAAVMAVA
                     IWMPGVGIMASNLLFSLGASMTLGGVAQMLAPKARTPRTQTTDNGKQNTYFSSLDNMV
                     AQGNVLPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            2722..6201
                     /locus_tag="JNC24_003608"
     CDS             2722..6201
                     /locus_tag="JNC24_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HAY9160265.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVDGPVDGLKS
                     VLLNGTPVLDSEGKTNLSGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRLTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVGNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGNQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTLKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVTDGFGGTEPRITCNSYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQMLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNAVEVNWIDPDNGWETATELVEDTQAIARYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISTGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGSGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDRRGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVAADDGSERLVSTARTTETTYRFRQ
                     LALGNYSLTVRAVNARGQQGDPASVSFRIAAPAAPVTIELIPGYFQITAVPKLAVYDP
                     TVQFEFWFSEKRIADIRQVETSARYLGTALYWIAASINIRPGHDYYFYVRSVNTVGKS
                     AFVEAIGRASDDAEGYLNFYKGLINKTHLGKELWTQIDNGQLAPDLTEIRTSITNVSN
                     EITQTVNKKLENQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMKDGRLYIAGIGAGIEN
                     TPAGMQSQVLLAADRIAMINPANGNTKPMFVGQGDQIFMNDVFLKCLTAPTITSGGNP
                     PAFSLTPGGRLTAKNADISGNVNANSGTLNNVTINKNCRVLGKLSANQIEGDLVKTVG
                     KPFPRDSRAPERWPSGTITVRVYDDQPFDRQIVIPAVAFRGAKHERKNNNIYSSCRLI
                     VKKNGAEIYNRTTLDNTLIYTGVIDMPAGHGHMTLEFSVSAWLVNGWYPTASISDLLV
                     VVMKKATAGITIS"
     gene            6269..6868
                     /locus_tag="JNC24_003609"
     CDS             6269..6868
                     /locus_tag="JNC24_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HAY9160266.1"
                     /translation="MRKVCAAILSAAICLAVSGAPAWASEHQSTLSAGYLQTHTDMPG
                     SDDLKGINVKYRYEFTDTLGLITSFSYANAEDEQKTHYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDGGRHSNTSLAW
                     GAGVQFNPTESVAIDLAYEGSGSGDWRTDGFIVGVGYKF"
     gene            6932..>7239
                     /locus_tag="JNC24_003610"
     CDS             6932..>7239
                     /locus_tag="JNC24_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004981847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY9160267.1"
                     /translation="MAVKISGVLKDGAGKPVVNCAIELRARRTSPTVVAHVVATCVTD
                     NNGAYVIEAEPGYYEVALHCNGWQPTRVGDIDVAPTDAPGTLNAFLNAPKDGDLRPEV
                     "
BASE COUNT         1637 a         1727 c         2315 g         1560 t
ORIGIN      
        1 tgctgatttg agtcgctaca gtggcacggt ggttaaccgt ccgacgtttt ttgcttttgc
       61 aaaaggcgcg ggtgtgatgg gggaagcggg acctgaagcc attctgccac tgcgtcgcgg
      121 tgctgatggt aagctggggg gtgtggcgga tactggtggt tcaggcatgg tgatgtttgc
      181 cccgcagtac aacattgaga tcaataacga tggcacgaac gggcagatag gtccggctgc
      241 cctgaaggtg gtttatgacc tcgggaaaaa agcggccgcg gactttatgc aacagcaggc
      301 ccgtgatggt ggccggttaa gtggagcata ccggtaatgg agacgtttca ctggaaagtg
      361 cgcccggata tgaatgtggt atcagagccg aaagtggtga cagtgaagct gggcgatggt
      421 tatgaacagc gtcgtgcggc gggactgaat aaccagttgt cgacttacag cgtgaagata
      481 cgtgttcgta aaggtgaaca cccattttta aaagcctttc tggaacggca cggtggcgtc
      541 cgcgcatttc agtggacgcc accttatgac tggaagccga tcagggtggt ttgtcgtaaa
      601 tggtcggcaa gcgtgggggc gttgtgggta accataacgg cagattttga acaggtcgtg
      661 gcataggagg ctctgatgca ggatattcca caggaaacac atcatgagac gacacgcctc
      721 actcagtcag cccaggtggt gctctgggaa atcgatctga cagaggtcgg tggtgaacgt
      781 tattttttct gtaatgagca gaacgaaaaa ggtgagccgg ttacctggca ggggcggcag
      841 tatcaggcat accccattca ggggacggga tttgaactga acggcaaggg cagtgctgcc
      901 cgtccgacac tgacggtttc taacctgcac ggaatggtca ccgggatggc ggaagacctg
      961 cagagtctgg tcggcggaac ggtggtcagg cgtaaggttt acgcccgttt tctggatgcg
     1021 gtgaacttcg tcaacggaaa cagcgacgcc gatccggaac aggaggtgat cagccgctgg
     1081 cgcatcgagc agtgcagcga actgagcgcg gtcagtgcct cttttgtgct ggccacacca
     1141 acggagacgg atggcgcggt tttcccgggg cgtatcatgc tggcgaatac ctgtatgtgg
     1201 acttaccgtt ctgatgagtg tggttacacg ggcagggcag tggctgacga gttcgacaaa
     1261 ccaacgacgg atatccggaa ggacaaatgc agcaagtgta tgcgcgggtg tgagttgcgc
     1321 aacaataccg gtaatttcgg cggtttcctt tccatcaata aactttctca gtaaatccat
     1381 gacacagaca gaatcagcga ttctggcgca cgcccggcga tgtgcgccag cggagtcgtg
     1441 tggcttcgtg gtaagaacgc cggagggaga caggtatctt cccagcgaga atatctccgg
     1501 tgagccggag gaacggttcc ggatggctcc ggaggactgg ctgcgggcac aaatgcaggg
     1561 tgagattgtg gcgctggtcc acagtcatcc cggtggtctg ccctggctga gtgaggctga
     1621 ccggcggctg caggtgcaga gtgatttgcc gtggtggctg gtctgccggg gggcgattca
     1681 caagttccgc tgtgtgccgc atctcaccgg gcggcgcttt gagcacgggg tgacggactg
     1741 ttacacgctg ttccgggatg cttatcatct ggcagggatt gagatgccgg attttcatcg
     1801 cggggatgac tggtggcgta acggtcagaa tctctatctt gacaatatgg aggtgacggg
     1861 tttttaccgt gtcgcactga cagaggcaca gccgggcgat gtgctgctgt gctgttttgg
     1921 ttcatcggtg ccgaatcatg ccgccattta ctgcggtgac ggcgggctgc tgcaccatat
     1981 tcctgaacaa ctgagcaaac gagagaggta caccgacaaa tggcagcgac gcacacactc
     2041 cctctggcgt caccgggcat ggcgcgcatc tgcctttacg gggatttaca acgatttggt
     2101 cgccgcatcg accttcgtgt gaaaacgggg gctgaagcca tccgggcact ggccacacag
     2161 ctcccggcgt ttcgtcagaa actgagtgac ggctggtatc aggtgcgcat tgccgggcgt
     2221 gatgcaggtg aaaccgaatt gtctgcccgt cttaatgagc cgctggcaaa tggtgcagtg
     2281 atccacatag taccgcgtct ggtgggagct aaaagtggcg gtgtgtttca ggcggtgctg
     2341 ggggcggctg ttatggcggt tgctatatgg atgccggggg taggaattat ggcgagtaat
     2401 ctgctgtttt ctctcggtgc cagtatgacg cttggcggtg ttgcacagat gctggcaccg
     2461 aaagccagaa ctccccgtac acagacaacg gataacggta agcagaacac gtatttctcg
     2521 tcactggata acatggttgc ccagggcaat gttctgcccg ttctgtacgg tgaaatgcgc
     2581 gtggggtcac gtgtggtttc tcaggagatc agcacggcag acgaagggga tggtggtcag
     2641 gttgtggtga ttggtcgctg atgcaaaatg ttttatgtga aaccgcctgc gggcggtttt
     2701 gtcgtttatg gagcgtgagg aatgggtaaa ggcagcagta aggggcatac cccgcgcgaa
     2761 gcgaaggaca acctgaaatc cacgcagttg ctgagtgtga ttgatgccat cagcgaaggg
     2821 ccggttgacg gtccggtgga tggattaaaa agcgtgctgc tgaacggtac gccggtcctg
     2881 gacagcgagg ggaagacaaa cctttccggt gttacggtgg tgttccgtgc aggtgagcag
     2941 gagcagacac cgccggaggg ttttgaatcc tccggctccg agacggtgct gggtacggaa
     3001 gtgaaatatg acacgccgat cacccggacc atcacgtcgg caaacatcga ccgtctgcgc
     3061 ctgaccttcg gtgtgcaggc actggtggaa accacctcaa agggtgaccg gaatccgtcg
     3121 gaagtccgcc tgctggttca gatacagcgt aacggtggct gggtgacgga aaaagacatc
     3181 accattaagg gcaaaaccac ttcgcagtat ctggcctcgg tggtggtggg taacctgccg
     3241 ccgcgcccgt ttaatatccg gatgcgcagg atgacaccgg acagcaccac agaccagctg
     3301 cagaacaaaa cgctctggtc gtcgtacacc gaaatcatcg atgtgaaaca gtgctacccg
     3361 aacacggcgc tggtcggcgt gcaggtggat tcagagcagt tcggtaacca gcaggtgagt
     3421 cgcaattatc atcttcgcgg gcgcattctg caggtgccgt cgaactataa cccgcagacg
     3481 cggcaataca gcggtatctg ggacggaacg cttaagccag catacagcaa caacatggcc
     3541 tggtgtctgt gggacatgct cactcatccg cgctacggca tggggaaacg tcttggtgcg
     3601 gcagatgtgg acaaatgggc gctgtatgtc atcggccagt actgcgacca gtcagtgacg
     3661 gacggatttg gcggcacgga gccgcgcatc acctgtaact cttacctgac cacacagcgt
     3721 aaggcgtggg atgttctcag tgatttctgc tcggcgatgc gctgtatgcc ggtatggaac
     3781 ggacagatgc tgacgttcgt gcaggaccga ccgtcggata aggtgtggac ctataaccgc
     3841 agtaatgtgg tgatgccgga cgatggcgcg ccgttccgct acagcttcag cgccctgaag
     3901 gaccgccata atgccgttga ggtgaactgg attgacccag ataacggctg ggagacggcg
     3961 acagagcttg tggaggatac gcaggccatt gcccgttacg gtcgtaatgt cacgaagatg
     4021 gatgcctttg gctgtaccag ccgggggcag gcacaccgag ccgggctgtg gctgattaaa
     4081 acggaactgc tggaaacgca gaccgtggac ttcagcgtgg gcgcagaagg gcttcgccat
     4141 gtaccgggtg atgtcattga aatctgcgat gatgattatg ccggtatcag caccggtggt
     4201 cgcgtactgg cggtgaacag ccagacccgg acgctgacgc tcgaccgtga aatcacgctg
     4261 ccgtcctccg gtaccacgct gataagcctg gttgacggaa gtggcaatcc ggtcagcgtg
     4321 gaggtccagt ccgtcaccga cggcgtgaag gtgaaagtga gccgggttcc tgacggcgtt
     4381 gcagaataca gcgtgtgggg gctgaagctg ccgacgctgc gccagcgtct gttccgctgc
     4441 gtgagtatcc gtgagaacga cgacggcacg tatgccatca ccgccgtgca gcatgtgccg
     4501 gaaaaagagg ccatcgtgga taacggggca cactttgacg gtgaccggcg cggcacggtg
     4561 aatggtgtca cgccgccagc ggtgcagcat ctgaccgccg aagtcaccgc agacagcggg
     4621 gaatatcagg tgctggcgcg atgggacacg ccgaaggtgg tgaagggtgt gagcttcctg
     4681 cttcgcctga ccgtggcagc ggacgacggc agtgagcggc tggtcagcac agccaggacg
     4741 acggaaacca cataccgctt caggcagctg gcgctgggga attacagtct gacagtccgg
     4801 gcggtaaatg cccgggggca gcagggcgat ccggcgtcgg tatcgttccg gattgcggca
     4861 ccggcagcgc ctgtcactat tgaactgata ccggggtatt ttcagataac ggcggtcccg
     4921 aaactggctg tatatgaccc gacggtgcag tttgagttct ggttttcgga aaagcggatt
     4981 gcggatatca ggcaggttga aaccagcgcc cgctatcttg gcacggcgct gtactggata
     5041 gccgccagta tcaatatcag gccgggccat gattattatt tttacgttcg cagtgtgaac
     5101 accgttggca aatcggcatt tgtggaggcc atcggtcggg cgagcgatga tgcagaaggt
     5161 tatctgaatt tttataaagg gttgatcaat aaaacgcatc tcggcaagga gttgtggacg
     5221 cagattgata acggtcagct tgcgccggac ctgactgaaa tcaggacgtc cattacgaat
     5281 gtcagcaatg aaatcacgca aaccgtcaat aaaaaactgg aaaatcagag tgctgcaatc
     5341 cagcagatac agaaagttca ggttgataca aataataacc tgaacagcat gtgggctgtg
     5401 aagctgcaac agatgaagga cggacgcctt tatattgcgg gtatcggtgc cggtattgag
     5461 aatacgccag caggaatgca gagtcaggtg ctgctggcgg cagacaggat tgcgatgatt
     5521 aatcctgcga atggcaacac aaagccgatg tttgttggtc agggcgatca gatattcatg
     5581 aacgacgtgt tcctgaaatg cctgacggct ccgaccatta ccagcggcgg taatcctccg
     5641 gcattttccc tgacacctgg cggacggctg acggcgaaaa atgccgatat cagcggtaac
     5701 gtgaacgcga actccgggac gctcaacaac gtcacgatta ataagaactg tcgggttctg
     5761 ggaaaattgt ccgcgaacca gattgaaggc gatctcgtta aaacagtggg caaacctttc
     5821 ccacgggact cccgggcacc ggagaggtgg ccatcaggga ccattaccgt cagggtttat
     5881 gacgatcagc cgtttgatcg gcaaattgtt attcccgcgg tggcgtttcg cggtgctaaa
     5941 catgagcgga agaataacaa tatttattcg tcatgccgcc tgatagtgaa gaaaaacggt
     6001 gctgaaattt ataaccgaac gaccctggat aatacgctga tatatacggg tgttattgat
     6061 atgcctgccg gtcacggtca catgacgctg gagttttctg tatcggcatg gctggtaaat
     6121 ggctggtatc ccacagcaag tatcagcgat ttgctggttg tggtgatgaa gaaagccact
     6181 gcaggcatca cgattagctg aattttataa cccagatacg ggcaccagaa atggtgcctt
     6241 ttttattgca gaaaagcgag aggtaattat gcgtaaagtt tgtgcagcca ttttgtccgc
     6301 agccatctgt ctggccgtat ccggtgcgcc tgcatgggcg tctgaacatc agtccacgct
     6361 gagcgccggg tatcttcaga cccatactga tatgccaggc agcgatgacc tgaagggcat
     6421 taacgtgaaa taccgttatg agtttacgga cacgctgggg ctgattacgt cattcagtta
     6481 tgccaacgct gaagatgagc aaaaaacgca ttacagcgat acccgctggc atgaggattc
     6541 cgtgcgtaac cgctggttca gcgtgatggc ggggccgtct gtgcgcgtga atgaatggtt
     6601 cagcgcgtat gcgatggcgg gcgtggctta cagccgtgtg tcgaccttct ccggggatta
     6661 tctccgcgta actgacaaca aggggaaaac gcacgatgtg ctgaccggaa gtgatggcgg
     6721 tcgccacagc aacacgtctc tggcgtgggg ggctggcgtg cagtttaacc cgaccgaatc
     6781 cgtggccatt gaccttgctt atgaaggttc cggcagtggt gactggcgca ctgacggttt
     6841 catcgtgggt gtcggttata agttctgatt agccaggtaa cacagtgtta tgactgcccg
     6901 ctggttcagg cgggcttttt gtggggtgaa tatggcagta aagatttcag gtgtactgaa
     6961 agatggggcg ggtaaacctg tcgtaaattg tgcgattgaa ctgcgggcca gaagaaccag
     7021 tccgaccgtt gtggcacacg ttgttgccac ttgcgtgacg gacaataacg gtgcttatgt
     7081 gattgaggct gagccggggt attacgaggt tgcgcttcac tgtaacggct ggcagccaac
     7141 ccgtgtcggg gatattgatg tggcaccgac tgatgcaccg gggacactga acgcgtttct
     7201 gaatgcacca aaggatggtg atttacgtcc ggaggtgat
//
LOCUS       DADMKF010000184         7121 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.187, whole genome shotgun sequence.
ACCESSION   DADMKF010000184 DADMKF010000000
VERSION     DADMKF010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7121
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.187"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            4..435
                     /locus_tag="JNC24_003611"
     CDS             4..435
                     /locus_tag="JNC24_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001409170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9160268.1"
                     /translation="MALNLASGEGNFFIRPGGVFYVAGDKVDIVRLDAFKTSKEIQFA
                     VQSGPMLMENSVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAKA
                     KLNVEQLLYLDGTISHMYMKGGAIPWQRYPFVTMISVERKG"
     gene            518..1420
                     /locus_tag="JNC24_003612"
     CDS             518..1420
                     /locus_tag="JNC24_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HAY9160269.1"
                     /translation="MKKTLATLFLLTCLGSSSAYADNALILQTDFSLKDGAVSAMKGV
                     AFGVDHNLKIFDLTHEIPPYNIWEGAYRLYQTASYWPQGSVFVSVVDPGVGTDRKSVV
                     LKTKNGQYFVSPDNGTLTLVAESLGIESVRQIDEKTNRLKGSEKSYTFHGRDVYAYTG
                     ARLASGAITFEQVGPELPAKVVELSYQKAKATKGEVKGNIPILDIQYGNVWSNISDEL
                     LNQAGIKLNDTLCVTISEGSQQKYAGKMPYVASFGDVPEGQPMVYLNSLLNVSVALNM
                     DNFAQKHQVASGADWNIDVKKCAK"
     gene            complement(1475..3637)
                     /gene="uvrD"
                     /locus_tag="JNC24_003613"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             complement(1475..3637)
                     /gene="uvrD"
                     /locus_tag="JNC24_003613"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HAY9160270.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAET
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTLHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLETV"
     gene            complement(3721..4437)
                     /gene="yigB"
                     /locus_tag="JNC24_003614"
     CDS             complement(3721..4437)
                     /gene="yigB"
                     /locus_tag="JNC24_003614"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HAY9160271.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            complement(4437..5333)
                     /gene="xerC"
                     /locus_tag="JNC24_003615"
     CDS             complement(4437..5333)
                     /gene="xerC"
                     /locus_tag="JNC24_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HAY9160272.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDVTMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHLHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            complement(5330..6037)
                     /locus_tag="JNC24_003616"
     CDS             complement(5330..6037)
                     /locus_tag="JNC24_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HAY9160273.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            complement(6034..6858)
                     /gene="dapF"
                     /locus_tag="JNC24_003617"
     CDS             complement(6034..6858)
                     /gene="dapF"
                     /locus_tag="JNC24_003617"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HAY9160274.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLAEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            complement(6895..7098)
                     /locus_tag="JNC24_003618"
     CDS             complement(6895..7098)
                     /locus_tag="JNC24_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAY9160275.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
BASE COUNT         1640 a         1990 c         1854 g         1637 t
ORIGIN      
        1 aaggtggcgt taaatctcgc ttcaggtgaa gggaatttct ttatccgtcc tggcggcgtg
       61 ttttatgtcg cgggagataa agtcgacatc gttcgtctgg atgccttcaa aaccagtaaa
      121 gagattcagt ttgcggtgca gtcagggcca atgttgatgg aaaacagtgt aattaatccg
      181 cgtattcatc ccaacgtcgc ctcacgtaaa attcgtaacg gtgttgggat taataaacat
      241 gggaatgccg tgtttttgtt gagccagcag gcgacaaatt tttatgattt tgcctgttat
      301 gccaaagcga aactgaacgt tgagcagctg ctttatcttg atggcacgat ttcacatatg
      361 tatatgaaag gcggggcaat cccgtggcaa cgctatcctt ttgtcaccat gatttccgtc
      421 gagaggaaag gttaagcatt gtacactcga ggaggccaga caaactgggg ctggctacac
      481 agctgaaaaa tcatcagata aatgaaggga aacaaaaatg aaaaaaacgc tcgcaacgtt
      541 atttttatta acgtgtctgg gatcatcatc cgcttatgcg gataacgctt tgattttaca
      601 aactgatttt agcctgaaag atggtgccgt ctcggcgatg aaaggggtcg cattcggtgt
      661 tgaccataat cttaaaatat ttgatttaac acacgaaatt ccaccgtata acatctggga
      721 aggcgcttac cgcctgtatc agaccgccag ttactggccg caaggttcgg tatttgtcag
      781 tgtggtcgat cctggtgttg gtacagaccg taaatcggta gtgctaaaaa ccaaaaacgg
      841 tcaatacttc gtttcaccag ataacggtac gctcactttg gtggcggaat cgctgggtat
      901 tgagagcgtc cggcaaatcg atgaaaaaac caaccgcctg aaaggttccg aaaagtcata
      961 taccttccac ggtcgtgacg tgtatgccta caccggtgca cgcctggctt ccggagcgat
     1021 cacgttcgaa caggtggggc cagagcttcc tgcaaaagtt gtcgaacttt cttatcagaa
     1081 agccaaagca acaaaaggtg aagtgaaggg caatatcccg attctcgata ttcagtacgg
     1141 caacgtctgg agcaatatca gcgacgagtt gctgaatcag gcaggaatca aactcaacga
     1201 cacgttgtgt gtaaccattt ccgaaggttc ccagcaaaaa tacgcaggga aaatgccgta
     1261 tgtcgcaagc ttcggcgatg tgccggaagg ccagccgatg gtgtatctaa acagcttatt
     1321 gaatgtttcc gtggcgctca atatggataa tttcgcgcag aaacatcaag ttgcgtctgg
     1381 tgctgactgg aatattgatg tgaagaagtg cgctaagtaa ggtgctgatg caggatgcgg
     1441 tgtggcgcac cgcatcctgc aacggagttg taaattacac cgtctccagc cgggcgtaag
     1501 ccgccaccag ccatttgatt ccctggccct ggaatgccac ctgcaaacgg ctatgttcac
     1561 cgctgccttc catattgacg atggtgcctt caccaaactt agcgtggcgc acgcgctggc
     1621 cgagtttgta gccgctgtcg ttctcgacca tcggcgtacc catccgctga tggctaaccg
     1681 ggcggcttac cgtggcgcgc aggcgcacct cttccacaca ctcttccggc agctcgccga
     1741 taaagcgcga cgggcgatgg taaacctctt tgccatacag acggcgagtt tccgcgtagg
     1801 tcagcgtcag tttctgcatc gcacgggtta cgccaacgta ggccagacgg cgttcttctt
     1861 ccagacgtcc gccttcatcc agcgacatct ggcttgggaa catgccctct tccataccga
     1921 cgataaacac ctgcgggaac tccagccctt tcgccgagtg tagcgtcatc aactgcaccg
     1981 catcctgcca agtatccgcc tgcccttcac ctgcttccag tgccgcatgg gagaggaacg
     2041 cctgcagcgg cattaaatct tcgtcttctt cgttgtagct gaactggcgc gttgccgtca
     2101 ccagttcctc taagttttcg atacgcgtct gacctttttc gcccttctcc tgctcgtaca
     2161 tggtacgcag gccggagtct ttaattaccc ggtcagtctg tacatgcagc ggcatatcgg
     2221 cagtttcttg cgctaaggca tcgattaatt ccataaatcg ctgcaaggcg ctggcagctc
     2281 gcccggcgag ggctttttcc tgcaacagtt cacgacatgc ctgccagagt gttaactggc
     2341 gatcgcgcga tgtctgacgt accacgtcca gcgtccggtc accaataccc cgcgttggcg
     2401 tattcaccac gcgctcaaac gccgcgtcgt cgttacggtt ggcaatcaga cgcagatacg
     2461 agagcgcgtc tttgatttcc tggcgttcga agaagcgcat cccgccgtaa atacggtacg
     2521 gcatactggc ctgcaacagt gcttcttcca gtacacgcga ctgggcgtta ctgcggtaga
     2581 gaatggcgca ctcggcaagc gccccgccgt tgtcctgcca agttttgatg cggttaacca
     2641 caaaacgcgc ttcgtcgagt tcgttaaaag cgcaatagag ggaaataggc tcaccgtccg
     2701 cgccatcggt ccacagtttt ttacccagac gcccgttgtt gttttcaatc agggcgttcg
     2761 cggcgctcag aatattgctg gtagagcggt agttctgctc cagacggata gtttcagcac
     2821 cggggaaatc attgaggaaa cgctgaatat tctctacctg cgccccgcgc cagccgtaga
     2881 ttgactggtc gtcatcgcca acgatcatca ctttgccggt atcgcccgcc agcaggcgga
     2941 tccacgcgta ctgaatgttg ttggtatcct ggaattcgtc caccaggatg ttggtaaaac
     3001 gttcgcggta gtgttgcagg atatgcggct tgttaagcca caactcgtga gcgcgcagca
     3061 gcagctcggc gaagtccacc aggcccgcac gatcgcacgc ttcctgatac gcctgataca
     3121 ccttctgcca ggtctgctcc accggattac cgtagctttg aatatgatgc ggacgcaggc
     3181 cttcatcttt ctggctgttg atgtaccaca ttgcctggcg cggcggccac tgcttctcgt
     3241 cgaggttcat ggctttgatc agacgcttaa gcaggcgtag ctggtcttca ctgtcgagga
     3301 tctggaaatc ctgcggtaga ttggcgtcca tatggtgcgc acgcagcaaa cggtgcgcca
     3361 gcccgtggaa ggtgccgacc cacatgccgc cctggctggt gcccatcagt tgcccgatac
     3421 gatgacgcat ctccgccgcc gctttgttgg taaacgtcac agccataatc gagtatggcg
     3481 agcagttttc cacgctcatc agccaggcga tacgatgcac cagtacgcgc gtcttaccac
     3541 tgcccgcgcc cgccagcacc agaaggttgc tgcgtggcgc ggccaccgct tcgcgctgtt
     3601 tgtcattaag gctgtcgagc aggtaagaaa cgtccattgg caccgccgcg taaaaatagg
     3661 ttgggcggag aagcgcaacg ccctccgcca aaaagctggg tatatataca gatttactga
     3721 ttatatcagc gaggtcagag atgccaaccg ggaaatttcc agatgcggca gtaaacggct
     3781 gtcccaggtt tgcatcagat cgccattttc cggtctgatc caacaagcct gcattccgct
     3841 gcgaattgcc ccacccacgt cagtggtgag atcgtcccca acatgtaaga tctcgccgat
     3901 cggcacgttg agtttttccg cagccaaaaa gtacatatcg ctgaacggtt ttgagcgccc
     3961 atgcgggcca gcgcgcagca caaactcaaa ataatccccc aaaccaaaca gttccggctg
     4021 ggcgttaccg ttggtgatcg ccaccagcgg ccatttcttc gccagctgtt ttaaggtgtc
     4081 gtgagtttgc tgcgggacgt cgattcggct gcgccatttg gcaaagttaa tcattgctgc
     4141 gtgtgcgcct gcactggctt cttcggcact cagcccggcg tcgagcatcg cttgttcaat
     4201 cgaacgaaaa cgccaacgcg tcacgtcgtg ataaatctcg ggttccgctt cccgtaccgc
     4261 ctggcgcagg cgttgcagat cttcattctg gaagctgcgc agcgccggat gataattttg
     4321 cacaaaggta agcgcctctc gctcggtgcg caaaatcacc ggacggttat cgtaaagggt
     4381 atcatccagg tcaaaggtga gcgccgagat gcgccccaaa ggccggtaaa aacgcattat
     4441 ttcccccgtt tggcgcgtgg atgcgccgca tcgtacaccg aggcaaggtg ttgaaaatca
     4501 agatgagtat agatttgcgt ggtggagagg ttggcatgac ccagcagctc ctgcacacca
     4561 cgaagatcgc cgctcgactc cagcatatgc gtggcgaacg agtgacgtaa tttatgcgga
     4621 tgaaggtgat tattcagccc ttgttttatg ccccattcgg caaagcgttt ctgcacatta
     4681 cgcgcggaga tacgcttgcc cagtttcgac agaaaaagcg cgtcgtcttc gctaccaaac
     4741 aggtcgcgca aatcaagcca gtgctcaatc cacgccacag cgttgcgacc aatcggcagg
     4801 cggcgctctt tgctgccttt ccccattacc cacacttcac cagactccag gtcgaggtgt
     4861 ttgatatcca gccccaccag ctcggaaaga cgcagacccg cgccgtacat cacttccagc
     4921 attgcacggt cgcgtacagc gaggggatcg ttgatatcaa tgtccagcag ccgattcata
     4981 tcgtcgacgt cgatattttt cggcagatga cgcggcgctt tcggtgccga aacacctttc
     5041 gccgggttag ctttgagttc gttctggctg accagccagt caaaaaagct acgtagcgca
     5101 gaaagccgta acgcgaggct tgctgctccc agccctttac ggcgactgcg tacagcaaaa
     5161 ttgcgcacca tcgtcacatc acattgctgc cagctttgca ggccgttttc gctggcaaaa
     5221 ttgatgatcg cctcaagctg acgctggtag ttaagcaggg ttatcgggct aagctggcgc
     5281 tccacgctca gataacgtag gtagcgttct acatcggtgt gtaaatcggt catacgcgtt
     5341 caatccaacg ctccagaagc tccggcaaca tcagcgcaat ttcatgaagt aactgcgttc
     5401 cctgcccttg ttgatagtga ctggcatcgc gactggtaaa cagcacgaca cccaaatcag
     5461 catcgcttcc cagcatcgac atcgccaccg atcccaccgc tttcgcttcc ggtagcacca
     5521 ccagcagctc tggtccgtta agcggcccaa gatagtgctg ttcctgcccc aaacgctgaa
     5581 tacgcagcgg ttcgaaagac tgacggctta atgccagatg agtgtggttc gacggcgcac
     5641 ctaagcgcca gcgatccgga aacaggcgca gactcgcacc tgccaggccg agatcgcgcg
     5701 cccagcggtg aaagcgcatc agcatatcgt cgagactgct ggcggcggtg agactgcgct
     5761 gcaggtagag taggcgataa aacaggcctt cgttggcgat agcctgttcc atcaacagcg
     5821 ccatgttctc ttccagaaca tgaatatgat tacgtgcgcg ggccatgtgc cactcgacca
     5881 acgaaacggt gccgcgtacc ggatgcggca cacgtatcgc ttctactgcg cgcgcattac
     5941 ggataaaaaa ctcaggattt ttaatcagat aatcgacaac cgcccggtca tcaagctccg
     6001 tgagtgtttc ctgcagttct tcccctggtt gcttcataga tgaataaatc cgtcgtagac
     6061 atgtaccgcc gggccagtca tatataacgg gtgacccgga cctttccagg cgatatcaag
     6121 acgaccgccg gggagttcca cgcgtacttc ttcggccagc aaaccttgct gaatccctac
     6181 tgcaaccgcc gcacacgcgc cgctgccgca ggcctgggtt tctcctgccc cacgctcata
     6241 aacgcgtaaa cgaatatgct cgcgcttaac cacttgcata aaaccgatat tggcgcgctc
     6301 cggaaaacgc tcgtggcttt ccagaacagg accaagcgtt tctaccgccg cggtatcgac
     6361 atcatcgacc tgaatcacgc aatgcggatt tcccatcgac accacgccgc ataagattgt
     6421 ctgctcggcg gcgcgcataa tataggtctt ttccgctttg ttagcgcgaa acggcacggc
     6481 ggaaggttcg aagttgggtt cgcccatatt tacgcggacc agatcatcat cggtgacggt
     6541 cagaaccatc cgcccgttgg cggtgctgac gcggatatca cgcttattgg tcagtccttt
     6601 cagacgcaca aaacgggcaa agcagcgcgc accgttgccg cactgcgcca cttcactgcc
     6661 atcagcattg aaaatgcgat agtgaaaatc cagttcagga tcatacggcg gctcaaccac
     6721 cagcagttgg tcaaacccta cccccaggtg ccgatcagcc aggcgacgaa tcagctccgg
     6781 tgaaaaaaag acattctgcg ttaccgcgtc gacgaccata aaatcgttgc caaggccatg
     6841 cattttcgag aactgcatca tttactccaa tcacgcgggt acagaaactg acttttagta
     6901 attcacctgg gatggaccat cgccagtggc gcgatcgttt ttatccggca ccgtggattg
     6961 cgtttgcgtc tctaccggtt tggtcggcgg cggtgcgttt ttatctgcag gcgggaaata
     7021 gagcggacct ttcagaccgc agcccgtcag gctgaagaga gtaagtaata cagtgagtgc
     7081 cttaaacacg tttttcatta tggattgcct gtaaagttca t
//
LOCUS       DADMKF010000185         6946 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.188, whole genome shotgun sequence.
ACCESSION   DADMKF010000185 DADMKF010000000
VERSION     DADMKF010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6946
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.188"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            7..309
                     /locus_tag="JNC24_003619"
     CDS             7..309
                     /locus_tag="JNC24_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HAY9160276.1"
                     /translation="MMPEYEGGFWHFIRLPDGGGYMMPDGDRFHMVNGENWFDRTVSA
                     DAAGIILTSLVINRQLWLYHDSGDAGLTHLYRMRDAQLWSHIEFHPECNAIYAALD"
     gene            356..778
                     /locus_tag="JNC24_003620"
     CDS             356..778
                     /locus_tag="JNC24_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 domain-containing protein"
                     /protein_id="HAY9160277.1"
                     /translation="MYCTVKEIIREVLDTDVPDSECVFAVVLTRGDVRHIARDWSLSD
                     DELETVMQRLDDAFVYGACDRVVSDIVNELMEEKRASRHVTVPAVMLEKVMALAGSEM
                     KRLYAVGSENGGDGDAFVREELEAMDVVLQALDGETMS"
     gene            775..966
                     /locus_tag="JNC24_003621"
     CDS             775..966
                     /locus_tag="JNC24_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9160278.1"
                     /translation="MNISTETREILRNYRAVINARRREMGQKPLTTAQIVDEICDFVA
                     NQQAVFLGGHYILQGSRNR"
     gene            1037..1351
                     /locus_tag="JNC24_003622"
     CDS             1037..1351
                     /locus_tag="JNC24_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005112260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9160279.1"
                     /translation="MRGAAPAALPARLSGQKVVYGCMQGTGVIFRDTVTVSGCLLLRP
                     LRLPAAWRHPVVRPDSDRQLLAHPPVSAEPAGAVSGCRYGPMRENPACSDRSGRAAPV
                     AP"
     gene            1735..2262
                     /locus_tag="JNC24_003623"
     CDS             1735..2262
                     /locus_tag="JNC24_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAY9160280.1"
                     /translation="MSARGINKVILVGRLGKDPEVRYIPNGGAVANLQVATSESWRDK
                     QTGEMREQTEWHRVVLFGKLAEVAGEYLRKGAQVYIEGQLRTRSWEDNGITRYVTEIL
                     VKTTGTVQMLGRAPQQNAQAQPKPQQNGQPQSADATKKGGAKTKGRGRKAAQPEPQPQ
                     PPEGEDYGFSDDIPF"
     gene            2320..2553
                     /locus_tag="JNC24_003624"
     CDS             2320..2553
                     /locus_tag="JNC24_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 domain-containing protein"
                     /protein_id="HAY9160281.1"
                     /translation="MSEYFRILQGLPDGSFTREQAEAVAAQYRNVFIEDDQGTHFRLV
                     VRQDGTLIWRSWNFEDGAGYWMNQYIRDFGILK"
     gene            2612..4570
                     /locus_tag="JNC24_003625"
     CDS             2612..4570
                     /locus_tag="JNC24_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000117326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HAY9160282.1"
                     /translation="MPVTKCEPETTRKASRKSVKTQETALSALLAQTEEVSVPLDSLI
                     KSPLNVRTVPYSAESVSELADSIKGVGLLQNLVVHALPGDRYGVAAGGRRLAALNMLA
                     ERNILPADWPVRVKVIPQELATAASMTENGHRRDMHPAEQIAGFRAMAQEGKTPAQIG
                     DLLGYSPRHVQRMLKLADLAPVILDALAEDRITTEHCQALALENDTARQVQVFEAACQ
                     SGWGGKPDVRVIRNLITESEVAVAENSKFRFVGADAFSPDELRTDLFSDDGDGYVDRV
                     ALDAALLEKLQAVAEHLREAEGWEWCAGRMEPVGECREDAGTYRCLPEPEAVLTEAEE
                     ERLNELMTRYDALENQCEESDLLEAEMKLMRCMAKVRAWTPEMRTGSGVVVSWRYGNV
                     CVQRGVQLRSEDDAADDADRTEQVQEKASVEEISLPLLTKMSSERTLAVQAALMQQPD
                     KSLALLAWTLCLNVFDSGAYSKPAQISLECKHYSLTSDAPSGKEGAAFLALMAEKARL
                     AALLPEGWSRDMTTFLSLSQEVLLSLLSFCTACSLNGVQTRECGRTSRSPLDSLESAI
                     GFHMRDWWQPTKANFFGHLKKPQIIAALNDAGLSGAARDAEKMKKGDAAEHAEFHMKD
                     NRWVPGWMCSPRPQTDATERADNLADAA"
     gene            4625..5059
                     /gene="psiB"
                     /locus_tag="JNC24_003626"
     CDS             4625..5059
                     /gene="psiB"
                     /locus_tag="JNC24_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000845897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="HAY9160283.1"
                     /translation="MNTELTLNALQSMNAQEYEEIRAAGSDMRRNLTHEVMREVDAPA
                     NWMMNGEYGSEFGGFFPVQVRFTPAHERFHLALCSPGDVSQLWMLVLVNGGGQPFAVV
                     QVQHIFTPVAISHTLALAATLDAQGYSVNDIIHILMAEGGQA"
     gene            5056..5775
                     /locus_tag="JNC24_003627"
     CDS             5056..5775
                     /locus_tag="JNC24_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001723065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
                     /protein_id="HAY9160284.1"
                     /translation="MSARSRALIPLSAEQQAAMQAVAVTEQRRRQGRTLSAWPYATAF
                     FRCLNGSRRISLTDLRFFAPALTKEEFHGNRLLWLAAVDKLIESFGEVCVLPLPSDAG
                     HRLFPSVPFREGERRRQKTTLTEQKYSRQREREAERRELEYQTCFAQAQIDLAFHTPA
                     TVGSWLSRWSGVVEEHDLETIFWGWCGRFPSLSSFDRFFWQEEPLWRLIFEAGEAGRG
                     APVQVRALEQWMIPNKLENVI"
     gene            5772..6368
                     /locus_tag="JNC24_003628"
     CDS             5772..6368
                     /locus_tag="JNC24_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000978013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9160285.1"
                     /translation="MMKSDEKYQVPAWMRPLLPLLCNTGGNDPEELLNDTETTASANV
                     VRYVLIVAVRSQVDLLQLLYRKGLLRTEIPGGFSPEEAQALLDNLVRSHISKALSGER
                     MAARDRNADLAWIRQQLVDAAWFVRATLEAHGMSVGNESPPAPPETMPDIQTRELVML
                     IKRLASSLKAVKPDSCVVREAQDWLRDRKLVDITDILR"
     gene            6440..6864
                     /locus_tag="JNC24_003629"
                     /pseudo
     CDS             6440..6864
                     /locus_tag="JNC24_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001672075.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1472 domain-containing protein"
     gene            6830..>6946
                     /locus_tag="JNC24_003630"
     CDS             6830..>6946
                     /locus_tag="JNC24_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001198918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HAY9160286.1"
                     /translation="MQYAKPVTLNVEECDRLFFLPYLFGQDFLYVEASVCALA"
BASE COUNT         1495 a         1851 c         2177 g         1423 t
ORIGIN      
        1 aaaaaaatga tgccggaata tgaaggcgga ttctggcact tcatccgcct gccggacggt
       61 ggcggttaca tgatgccgga tggcgaccgt ttccacatgg tgaacggtga aaactggttt
      121 gaccgcacgg tcagtgctga tgccgcaggc atcatcctta cctcccttgt gattaaccgc
      181 cagttgtggc tgtaccacga cagcggggat gcagggttga cacacctgta caggatgcgt
      241 gatgcgcagt tgtggagcca catcgaattt caccctgaat gcaacgcgat ttacgcggcg
      301 ctggactgat taacggacgg ggcggcaatg ccgcccctgt aaaggagacg agaacatgta
      361 ctgtactgtt aaagaaatta tccgcgaagt gctggataca gacgtgccgg acagtgaatg
      421 cgtttttgcc gtagtgctga cccgtgggga tgtgcgtcac atagcccggg actggagcct
      481 gtcagacgat gagctggaaa ccgtcatgca gcggctggac gatgcttttg tatatggcgc
      541 atgtgacaga gttgtttccg atattgtcaa tgaactgatg gaagaaaagc gcgccagccg
      601 tcatgtgaca gtcccggcgg tgatgctgga aaaagtgatg gcgctggcag gtagtgaaat
      661 gaagcgcctg tatgccgtcg ggagtgagaa cgggggcgac ggtgacgcat tcgtcaggga
      721 ggaactcgaa gcaatggacg ttgtgttgca ggcgctggac ggggagacga tgtcatgaat
      781 atcagcacag aaacccgcga aattctgcgt aattacaggg ccgtgattaa tgcgcggcgt
      841 cgtgaaatgg ggcagaaacc gctcaccacc gcgcagattg ttgatgaaat ctgcgatttt
      901 gtggcgaatc agcaggcggt tttcctgggc ggtcactaca tcttacaggg cagcagaaac
      961 aggtgataca gggcaggcgg tggaggccgc ctgtcagccc gcgcgaaaac ccctggcagg
     1021 ctggcgcctg ccggaaatgc ggggtgcagc gcctgcggcg ctgccggccc ggctttccgg
     1081 tcagaaggta gtgtatggct gtatgcaggg aacaggtgtg atattccgtg acactgtcac
     1141 cgtttctggc tgtcttttat tgcgcccact tcggctcccg gctgcctggc ggcatccggt
     1201 cgtcaggccc gactccgaca ggcagctgct ggcgcacccg ccagtctccg ccgaacccgc
     1261 tggcgcggtt tcgggctgcc gctacggtcc gatgcgtgaa aacccggctt gcagcgaccg
     1321 ttccggtcgc gccgcccctg tcgcgccgtg acctgcgctc cctgcacacc tgacggtgtg
     1381 tgaggaaact gcgcctgatg gcgcagcagt cttccctcct gccgccacct ctgcggcctg
     1441 tttcccgctg tggactttcc ggggggcaga cgtaaccccc tcccgttcca tctgtccagg
     1501 gtgagatgca ccggccacaa aatttttagc catgaacccg gcaaaaaatt ttgcgtccgc
     1561 ccccggacag ctgtcccgtg aggcggtaaa cgggccccca tcggaaaggt ccactgcggg
     1621 gagaaaaccc tgtgaagtgg acggcaggag gagctttcgc tcctctgaag ggggacatga
     1681 acgtcccgtc gccggtatcc ggtttatctc acatctttca gtcaggaggt tcttatgagt
     1741 gcacgtggta tcaacaaggt catcctcgtc gggcgtctgg gaaaagatcc ggaagtccgt
     1801 tacatcccaa acgggggcgc agtggcaaac ctgcaggtgg ccacgtcaga aagctggcgt
     1861 gacaaacaga cgggggagat gcgggagcag acagaatggc atcgcgtggt gttgttcggc
     1921 aagctcgcgg aagtggcagg tgaatatctg cgcaagggtg cgcaggtcta catcgaaggt
     1981 cagcttcgca cccgtagctg ggaagataac ggtatcaccc gttacgtcac tgaaattctt
     2041 gttaagacca cgggcaccgt gcagatgctg ggacgtgcac cacagcagaa cgctcaggcg
     2101 caaccgaagc ctcagcagaa tgggcagcca cagagtgctg acgcgacgaa aaaaggtggc
     2161 gcgaaaacga aaggccgtgg acgtaaggcc gcgcagccag agcctcagcc gcaaccgccg
     2221 gagggggagg attacgggtt ttcagacgac atcccgttct gatcgggctg actgtgacaa
     2281 ccgccccgcc ctgtgcgggg catcaccgga gatatgagga tgagcgaata tttcagaata
     2341 cttcagggac tgccggacgg ctcctttacc cgcgaacagg cggaagccgt tgccgcacag
     2401 taccggaacg tctttatcga ggatgatcag ggaacgcatt ttcgcctggt tgtccgtcag
     2461 gatggcacgt tgatctggcg ctcctggaat tttgaggacg gtgccgggta ctggatgaac
     2521 cagtacatca gggatttcgg gattcttaag taagagaggt gccggacgcg cgaacgtccg
     2581 gcaggacata agcaattata aggggatgat tatgcctgta accaagtgtg aaccagaaac
     2641 cacccgcaaa gcaagccgta aatctgtaaa aacgcaggaa actgcactgt ctgccctgct
     2701 ggcgcagacg gaggaagtga gcgtgccgct ggattcactg attaaatcac cgctgaatgt
     2761 gcgcacggtg ccgtattctg cggagtccgt cagtgaactg gctgattcca ttaagggagt
     2821 cggcctgctg cagaatctgg ttgttcatgc cctgccaggt gaccgttacg gtgtcgccgc
     2881 aggcggtcgc cgactggcag cactcaacat gctggcagag cgcaacatcc ttccggctga
     2941 ctggcctgta cgcgtgaagg tcattccgca ggagctggcg actgccgcat caatgactga
     3001 aaacggtcat cgtcgggata tgcaccctgc cgaacagatt gccggattcc gtgcgatggc
     3061 gcaggaaggc aaaacgcctg cacaaatcgg tgatttgctg ggctattcgc cccgccacgt
     3121 tcagcgaatg ctgaaactgg cagaccttgc gcctgtcatc ctcgatgcgc tggcagaaga
     3181 ccgcatcacc accgagcact gtcaggcgct ggcgctggag aacgacaccg cgcgtcaggt
     3241 acaggtgttt gaagccgcct gtcagtcggg atggggcgga aaaccggatg tgcgggttat
     3301 ccgcaatctg attacggaaa gtgaagtggc ggtggcggag aacagtaaat tccgcttcgt
     3361 gggggctgat gctttctcgc cagacgaact gcgcactgat ttgttcagcg atgacgggga
     3421 cggttatgtc gaccgcgtgg cgctcgatgc tgccctgctg gaaaaactcc aggctgtcgc
     3481 tgaacacctt cgggaagccg aaggctggga atggtgcgcc ggacgcatgg agcctgtcgg
     3541 tgagtgccgt gaggatgccg gaacataccg ctgtctgccg gagccggaag cggtgctgac
     3601 ggaggcggaa gaagaacgcc tgaacgaact gatgacgcgt tacgacgcgc tggaaaacca
     3661 gtgtgaggaa tccgacctgc tggaagcaga aatgaagctg atgcgctgca tggcgaaggt
     3721 cagagcgtgg acgccggaga tgcgtaccgg aagcggtgtg gtggtgtcct ggcgttacgg
     3781 taatgtgtgt gtccagcgtg gtgtgcagtt gcgcagcgag gatgacgcgg ctgacgacgc
     3841 tgaccgcacg gaacaggtgc aggagaaagc atcagtggag gaaatcagtc tgccactgct
     3901 gacgaaaatg tcttccgaac gcacgctggc agtccaggcg gcactcatgc agcaaccgga
     3961 caaatccctg gcactgctgg catggacgct ctgcctgaat gtgtttgaca gcggagcgta
     4021 cagtaaacca gcacaaatca gcctggaatg taaacattat tcgctgacca gcgatgcgcc
     4081 gtcagggaag gaaggtgccg cattcctggc gctgatggca gaaaaagccc gtcttgctgc
     4141 cctgttaccg gagggatggt cacgggacat gacgacgttc ctgtcactca gtcaggaggt
     4201 gctgttatcc ctgctcagtt tctgcaccgc atgcagcctt aacggtgtcc agacccgcga
     4261 gtgtggtcgc acgtcacgca gtccgcttga ctcactggag agcgccatcg gttttcacat
     4321 gcgcgactgg tggcagccga cgaaagcaaa cttcttcgga cacctgaaaa agccgcagat
     4381 tatcgcagcc ctgaatgatg caggactgtc cggtgccgca cgggacgcag agaagatgaa
     4441 gaaaggcgat gccgctgaac atgcagagtt ccatatgaaa gacaaccgct gggtgcctgg
     4501 ctggatgtgt tcaccacgtc cacagacgga tgccactgaa cgcgccgata acctggctga
     4561 tgccgcctga tgaacaacca caccgccccg ccggagacgg ggcggcagag ggagaaatac
     4621 gatgatgaat accgaactga cactgaatgc cctgcagtcc atgaatgccc aggaatatga
     4681 agaaatccgt gctgcgggaa gcgatatgcg ccgtaatctc actcacgagg tgatgcgtga
     4741 agtggacgca ccggctaact ggatgatgaa cggagagtat ggcagcgagt tcgggggctt
     4801 tttccccgtc caggttcgtt tcacgccagc ccacgaacgt ttccacctgg cattatgttc
     4861 gccgggagac gtctctcagc tctggatgct ggttctggtg aatggtggtg gacagccttt
     4921 cgccgtcgtt caggtgcaac atatcttcac gcctgtcgcg atcagtcaca cgctggcgct
     4981 tgccgcgaca ctggatgcgc agggatatag tgttaacgac atcatccata tcctgatggc
     5041 agaaggaggt caggcatgag cgcacgttca cgggcactga tccccctcag cgcagaacaa
     5101 caggccgcca tgcaggcggt ggctgtcaca gaacaacgtc gtcgtcaggg acgcacactt
     5161 tcagcatggc cttatgccac cgctttcttt cgctgcctga atggcagtcg ccggatttcg
     5221 ctgaccgatc tccgcttttt tgcccctgcg ctgacgaagg aggaatttca tggcaaccgt
     5281 ctcctgtggc tggctgccgt ggataaactg attgaaagtt ttggggaagt ctgtgttctt
     5341 cccctgccat ccgatgcggg gcaccgtctg ttcccgtccg tgccttttcg tgaaggtgag
     5401 cgtcgtcgtc agaaaaccac gctgacagag cagaaataca gccgccagcg ggaacgtgag
     5461 gcagaacgac gggaactgga ataccagaca tgttttgctc aggcgcagat tgaccttgcg
     5521 tttcatactc ccgccacggt cggaagctgg ttgtcccgct ggtctggtgt tgttgaggag
     5581 catgatctgg aaacgatttt ctgggggtgg tgcgggcgtt ttccatcact gtcatcattt
     5641 gaccggtttt tctggcagga ggaaccactc tggcggctga tttttgaagc cggtgaggcc
     5701 ggtcgtggtg caccggtaca ggtacgtgca cttgagcagt ggatgatccc gaacaagctg
     5761 gagaacgtaa tatgatgaaa tcagacgaaa aataccaggt tcccgcgtgg atgcgacctc
     5821 tgttgccgtt gctctgcaac accgggggga acgatccgga agaactgctg aatgatacag
     5881 aaaccactgc cagtgcgaat gttgtccgtt atgtactgat agttgctgtg cggtcgcagg
     5941 ttgatctgct gcagcttctg tacaggaaag ggctgttgcg cacagagata ccaggtggct
     6001 tttcaccgga agaagcgcag gcactcctgg ataatctggt gcgcagccat atcagcaagg
     6061 cgctgtcggg cgagcgaatg gcagcccgtg acagaaatgc cgatctggcc tggattagac
     6121 agcaactggt cgatgccgcc tggtttgtcc gtgccacact ggaagcgcat gggatgagcg
     6181 tcggaaatga gagtccccct gctccgccgg agacaatgcc ggacatacag acacgggaac
     6241 tggtgatgtt gatcaagcga ctggcatcat cgctgaaagc ggtgaaaccg gacagttgtg
     6301 tggtgcgtga agcgcaggac tggctgcgcg acagaaaact tgtggatatc acagatattc
     6361 tccggtgaac caggcatgta tattttacgc agggacatgc cggtcatgat cgtttcgttc
     6421 caggggtggt ctggagatca tgcggtgtgc tccctgctct cgccggaata aggacgtcgc
     6481 cgccggctcc gcttttaccc ggccatgcgg ggcatggcct tgtgggtttt cagttatgtg
     6541 gcctcagcat cgtgtgcggg ctgtgccgtg cctccatcgt agccgggctt ctgcgggcgg
     6601 gttgcaaggt aaggtgaacc gctgcggtca cccggtccct ccttcccgtc tgccgtgatt
     6661 ttccggtcgt tcacccgctc agatttcgcg gtctcaccct ccaactcccg ccagccgtgt
     6721 gcgtcaggct gcggcttccc ttgcgccctt gcaaccctgc aggcccggca aagatggagg
     6781 cacggcaccg cccggtgtcg cggaatatgt gaacaacgga ggaaatccca tgcagtatgc
     6841 gaaacctgtc actctgaatg ttgaagagtg cgaccgtctg ttttttctgc cttacctgtt
     6901 tggccaggat tttctgtatg tcgaagcgtc tgtgtgcgcg ctggcg
//
LOCUS       DADMKF010000186         6877 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.189, whole genome shotgun sequence.
ACCESSION   DADMKF010000186 DADMKF010000000
VERSION     DADMKF010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6877
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.189"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(91..264)
                     /locus_tag="JNC24_003631"
     CDS             complement(91..264)
                     /locus_tag="JNC24_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9160287.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDI"
     gene            365..550
                     /locus_tag="JNC24_003632"
     CDS             365..550
                     /locus_tag="JNC24_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9160288.1"
                     /translation="MSRRYSSATITLVILFCRRPHCRKISVRQYYLKRVIFYPTTLQR
                     TAFKKCLFLMRDIIISS"
     gene            511..630
                     /locus_tag="JNC24_003633"
     CDS             511..630
                     /locus_tag="JNC24_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAY9160289.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            complement(1109..1375)
                     /gene="minE"
                     /locus_tag="JNC24_003634"
     CDS             complement(1109..1375)
                     /gene="minE"
                     /locus_tag="JNC24_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HAY9160290.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(1379..2191)
                     /gene="minD"
                     /locus_tag="JNC24_003635"
     CDS             complement(1379..2191)
                     /gene="minD"
                     /locus_tag="JNC24_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HAY9160291.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            complement(2215..2910)
                     /gene="minC"
                     /locus_tag="JNC24_003636"
     CDS             complement(2215..2910)
                     /gene="minC"
                     /locus_tag="JNC24_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HAY9160292.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALEDKIAQAPAFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTTPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            3430..3798
                     /locus_tag="JNC24_003637"
     CDS             3430..3798
                     /locus_tag="JNC24_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9160293.1"
                     /translation="MNMMRIIYIGLSGVGMMFSSMASGHDAGGLQSPACGVVCDPYIC
                     VNSDGISPELTRKYLGEKAAENLQSLQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVD
                     GKRSGKINQTTTKMLFMCRE"
     gene            complement(3906..4307)
                     /locus_tag="JNC24_003638"
     CDS             complement(3906..4307)
                     /locus_tag="JNC24_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inhibitor of g-type lysozyme"
                     /protein_id="HAY9160294.1"
                     /translation="MKIKSISKAVLLLALLTSTSFAAGKNVNVEFSKGHSSAQYSGEI
                     KGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPGIDDSVDLSRYSPELDSHGQYSLP
                     ASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            4549..4842
                     /locus_tag="JNC24_003639"
     CDS             4549..4842
                     /locus_tag="JNC24_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9160295.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKLALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            4914..5573
                     /locus_tag="JNC24_003640"
     CDS             4914..5573
                     /locus_tag="JNC24_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HAY9160296.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFISAAEFTGDPQNTTLGLSVNGEQRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            5650..6111
                     /locus_tag="JNC24_003641"
     CDS             5650..6111
                     /locus_tag="JNC24_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415699.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HAY9160297.1"
                     /translation="MAEHLMSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDED
                     TDEIYFTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMICAYRLLA
                     EGKDLPAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(6318..>6877)
                     /gene="hlyE"
                     /locus_tag="JNC24_003642"
     CDS             complement(6318..>6877)
                     /gene="hlyE"
                     /locus_tag="JNC24_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hemolysin HlyE"
                     /protein_id="HAY9160298.1"
                     /translation="VLDDGITKLNEAQKSLLVSSQSFNNASGKLLALDSQLTNDFSEK
                     SSYFQSQVDKIRKEAYAGAAAGVVAGPFGLIISYSIAAGVVEGKLIPELKNKLKSVQS
                     FFTTLSNTVKQANKDIDAAKLKLTTEIAAIGEIKTETETTRFYVDYDDLMLSLLKEAA
                     KKMINTCNEYQKKHGKKTLFEVPEV"
BASE COUNT         1780 a         1507 c         1614 g         1976 t
ORIGIN      
        1 cctttcgtgg tgcggttcat atccgccatc gctggcgtgg agcagaacaa tgctgagata
       61 agcccgaagg caagtatttt tttcttcaac ttagatgtcc ggtattaagt aagttgcaca
      121 cacaataatt tcgtcttcaa ttaagatctg cttaactaaa gaacgctcgc tattattcag
      181 ataattcaaa atgagcgtgg ctgtgatgat aggaattatg ttttttacgt gaatgagaat
      241 aatcttaaat gaggaataac tcattgattg gcaatatttt tattcaagaa gtatcattga
      301 ctgttaacgc aatgttgtaa aggtaagata atctgattta tcaatattat tgtgtgattt
      361 ttatgtgagc agaagatatt catcagcaac gattacatta gtcattttat tttgccgacg
      421 gcctcattgt cgaaagataa gcgtacgaca gtattatctg aaaagagtga ttttttatcc
      481 aactacactt cagcgcactg cgtttaaaaa atgcctcttt cttatgcggg atatcatcat
      541 ttcatcatga tgtctttggt gagcggtgaa cacaatacac ctgcgctgtc tcttcaggat
      601 gaatcccctg gtctggtgcc tgcgggctga tgttgcagca gagcttaggt cacttagacg
      661 ctactatcat ttatccaatg gcatggaatc gaaatcagtc gatacccgca gtatatatcg
      721 tgaactggtc gttctgacca tcaacgatta aactgcgctt cggcgcagtt ttcgtttaca
      781 gggggctaaa agggaaaatt ctggggcaaa aaaagcccgc cagttacggc gggaaacctc
      841 atcctatggg agaacaatga ataatgaaat tgcggggtta tcatccccca gtgtatccac
      901 actaacaata aggttattta ctcagccagg cataaacatt atgttctgtg cgtgggaaca
      961 gccttaaggt gtaaaggggg aggtggaaat agcaatgagg agtatcagca agaatactcg
     1021 ccgctttacc acaacgtgga tgagagggat gaaaaactca aggcagaaat gactctgcct
     1081 tgaagataaa tgcgttttta cagcgggctt atttcagctc ttctgcttcc ggtaaggtca
     1141 cgttcagctc aagaatagaa atatcgccat ctttttgctc aagctgtacg gttaccatct
     1201 caggatcaat ttgtacgtat ttacaaatga cctcaagaat atctttacgc aactgcggca
     1261 gataatgcgg ttctgcatcg ctgcgacggc gttcagcaac aataatctgc agccgttctt
     1321 ttgcaatgtt ggctgtgttt ttcttccgcg agagaaagaa atcgagtaat gccataactt
     1381 atcctccgaa caagcgtttg aggaagcctt tcttctcttc ttcaatgaag cggaaaggac
     1441 gttcttctcc caacagacgt tctacggtat ctgcgtaggc tttacccgca tcggcgttaa
     1501 tgtcgagaat gaccggttca ccctggttag aggcgcgtaa tactgattga tcctctggga
     1561 tcacgccgac gagtttgatg cgcaggatct ccagcacatc ttccatgctc agcatgtcac
     1621 ctctgcttac gcggcctggg ttatagcgcg ttaacagcag gtgctcttta ataggctctt
     1681 cgccattttc tgcgcggcgt gatttcgacg ccagaatgcc taaaatacgg tcagagtcgc
     1741 gtactgagga gacttccggg ttggtggtaa taatggcttc gtctgcaaaa tagagtgcca
     1801 ttaacgcacc ggtttcaatc cctgccgggg agtcacaaac gataaattca aaatccatcg
     1861 ctttcagatc atcaagaact ttggcgaccc cttcacgggt gagggcatct ttatcgcgtg
     1921 tttgcgatgc cggaagaata tagagatttt cagtacgctt atctttaatt aacgcctgat
     1981 ttagcgttgc atcgccctga atgacgttga cgaaatcgta aacgacccgg cgttcgcaac
     2041 ccataatcag gtcgagatta cgcaggccga tatcaaaatc tatcacgaca gttttctttc
     2101 ccttctgggc caaaccagtg gcgatggccg cgctggaggt tgtcttacca accccccctt
     2161 tgcccgaagt aacaacaata atgcgtgcca tagaaattcc ttgttaaaaa gggatcaatt
     2221 taacggttga acggtcaaag cgttttcgac taactgcagt cgcgccgctt tgccataaaa
     2281 ttctgctggg atttgatcac tcagccagta ttcacctgcg atggacacca attccgccat
     2341 caggttcgta caaaatattt gcgtttcccg gtcaccactt gcccctgcca gcgcacgacc
     2401 gcgcatcatg ccatagacat gaatgttccc atcggcaatc aattcggccc cagcgctaac
     2461 gtggcttgta acaatcagat cacattgtgg agcataaata cgctgaccgg aacgcaccgg
     2521 ggtatctatt aaacgcgttt ttgtgaccgg cgtggtattt tgcgctggag cctgtggtgc
     2581 gggagctgga cgtggcgctt tttcctttcc ttccgtcagg ataggcagcc ccattttttc
     2641 aatttcggct ttaagttgcg cgtctttgca gccacttacg ccaataaccc gcaaaccggt
     2701 tgccgaaacc gccttatgca tcgctgacca gtttaccggg tcttccagtg cactgacgtt
     2761 gagtacaacg ggggcatgtt ttaaaaatgc gggggcctga gcgattttgt cttccagcgc
     2821 ctgatggata accttaggtt ctgcctcatg cagatgaacc acagataaag tgaagctact
     2881 gcctttaagc tcgattggcg tgtttgacat cctggcctta ctcaattagc tattaatcat
     2941 cgccagcgcg cgatgatgtt ccgaagacta taaggcatgt tatagtctgg attatattga
     3001 ggcaagtcac cctcccattt attcagagta aaagtctatt ctgtgataaa tggcgctgat
     3061 tcatagctta aaaaataccc ttgtcaatca acccattgcc gccgtacttt tgattcttct
     3121 tatttacgct tcctttttcc gcaccctaat cgtgacagca tctccagacc tgtttaaata
     3181 tccagcattg aggcaagtgt attttttcac cacgccaggc tttcttaact ttccattttt
     3241 tgcagcatca ttaaagaaca gatacccgtt atcttaatat ccgccacaga attgctctat
     3301 catagaaacc gcagaagacg tctgatactt ctgtcaggtc atcgtaactc gtccaggtca
     3361 gcggttttga ttacggattg atgacgaaat gagcataagt gttgtacggt tggattatca
     3421 ggatgtgcta tgaatatgat gcgtattatt tatatcggat tgtcaggtgt ggggatgatg
     3481 ttctcatcaa tggcgtctgg acatgacgct ggcggacttc aatctccggc gtgcggcgtt
     3541 gtttgcgacc cgtatatttg tgtgaactca gatggcattt ccccagagtt aacaaggaaa
     3601 tatctcggcg aaaaagccgc tgaaaactta caatcattac aaggctacga tcccagcgaa
     3661 tttacattcg ctaacggtgt attttgcgat gttaaagaaa aattatgtcg tgatgatcgt
     3721 tattttggtg tagatggtaa gcggagtgga aaaatcaatc aaaccaccac aaagatgtta
     3781 tttatgtgtc gtgagtgatg ccttgtttac attatgttct ctgctattcg tcattaacct
     3841 cttgagcgac agagaggagt ggggcgttaa cgtctatgat ggggatagtc cccgtcggtt
     3901 gacatttact taatttgaat atcgacgtta tattttttag ttttgttttt acgggcgtcg
     3961 tttcgagttt gcagcactcg cagctcatat ttgccagaag cagggagtga gtattgtccg
     4021 tgactatcga gttctgggga atatctggat aaatcaacag aatcatcgat tcctggcccg
     4081 aagagataag tatcagcgcc ttcattagag atactcacat gcactttctg accttttttg
     4141 gcatagaagg tatatgtatc gtaatcgtat ccctttattt cgccggagta ctgagcgctg
     4201 ctatggcctt tgctgaactc aacattgaca tttttacccg cagcaaagct cgtggaggtt
     4261 agcagtgcaa gcagcaatac agccttactg atgctcttga ttttcattgg tagtctcctg
     4321 ttcagaatga gtacgatgaa ctgttataat ataacaatcc cttacgggag aattccagtg
     4381 gcttttctgc gttcggtaaa atcccggcgc ggcatttttt gccgggggga ttacttataa
     4441 aggggcatgg actcaacaaa ccgcagaaat ctacagctgg ttggcttgtg gcgcgggttt
     4501 cattgatgtt agcttatgcc aaaacctggc atcttaaaga gtaaaagtat gttttgtgtg
     4561 atttatcgaa gcagcaagcg tgaccagacc tatttatatg tcgaaaaaaa agacgatttt
     4621 tcgcgtgttc ctgaggaact gatgaaaggt tttggtcagc ctcagttagc gatgattctg
     4681 ccgttggatg ggcggaagaa actggtcaat gctgatattg aaaaggttaa actggcatta
     4741 accgagcaag gttactattt gcagttaccg ccaccacctg aagatttgct gaagcaacat
     4801 ctttccgtca tggggcagaa aacagacgac actaacaaat aaccgatatc cggcggtggc
     4861 attatctttg tcggcgcggg ttttcatatc cacgataagg tgaggggaac gttatgtatc
     4921 aacatcacaa ctggcaaggt gcgctgctgg attatccggt gagtaaagta gtctgtgttg
     4981 gcagtaacta tgccaaacat attaaagaga tgggcagcgc agtgcccgaa gagccagtgc
     5041 tgtttattaa accagaaacg gcactgtgcg atctgcggca gccattggcg atcccatccg
     5101 atttcggttc agttcatcat gaagtcgaac tggcggtgtt gattggcgcg acgctgcgtc
     5161 aggcaacgga agagcatgtc cgcaaagcca ttgccggtta cggcgtggcg cttgatctga
     5221 cattgcgtga tgttcaggga aaaatgaaga aagccgggca gccgtgggaa aaggctaaag
     5281 cgtttgataa ctcttgtccg ctttccgggt ttatctccgc ggcggaattt accggcgatc
     5341 cacaaaatac aacgctgggc ctgagcgtaa acggcgaaca acgccagcaa ggtacgactg
     5401 cggacatgat ccataaaatc gttccgttga tcgcttatat gagcaagttt tttaccctca
     5461 aggccggtga cgttgtgttg acaggcacgc ctgatggcgt cggcccgttg caaagcggtg
     5521 atgagctgac agtcactttc gatgggcatt ctttgacaac tcgcgttttg taatactttt
     5581 tgccgcctga aagcggcggc aaaacttgca tcgctgtgcc agactggtta taaggtgcgt
     5641 tttaacgtaa tggcggaaca cctgatgagc gatgtacctt tctggcaaag taaaaccctg
     5701 gacgaaatga gcgatgcgga atgggagtcg ttgtgtgatg gttgcggtca gtgttgcctg
     5761 cataaactga tggatgaaga caccgacgaa atctacttca ctaacgtcgc ctgtcgccag
     5821 ctcaatatta aaacttgtca atgtcggaac tacgaacgtc gtttcgagtt tgaacccgac
     5881 tgcattaaat taacccgtga aaatctgcca acattcgaat ggctgccaat gatctgcgct
     5941 tatcgtttgc tggcggaagg taaagattta cctgcgtggc atccgctact tactggttcg
     6001 aaagcggcaa tgcatggtga acgtatctct gtgcgtcata tcgcagtgaa agaatcagaa
     6061 gtgattgact ggcaggatca tatcttaaat aaacctgact gggcgcagtg ataaatttaa
     6121 tagatgttga tgggggcttt cttaatatac gtctggatgc tcgccatttc gtagccctgt
     6181 ttttgaaagc gctctgtcat aatgagagtt acccgatacc ttattatcaa caggtaactc
     6241 tcaatcttta aaaaacgtct gtgtttttaa atgtgataaa ccttatacct tgagtacatg
     6301 gagagaataa tcgctgatca gacttcaggt acctcaaaga gtgtcttttt accgtgtttt
     6361 ttctgatact cattacaggt gttaatcatt tttttggccg cttcttttag caaagaaagc
     6421 attaaatcat cataatcaac gtagaatctg gtggtttcag tttccgtttt tatctctccg
     6481 atggcggcta tttcggtggt taatttcaat ttggcggcat cgatatcttt attcgcttgt
     6541 ttaaccgtgt tagacagggt ggtaaagaaa ctctgcacag attttaactt gttcttcaat
     6601 tctgggatca gtttcccttc aactacgccc gcagcaatag aataggaaat gattaaacca
     6661 aatggaccgg cgacgacacc ggctgcggca ccggcatacg cttccttcct gattttatct
     6721 acctgtgact ggaaatagct gcttttttct gaaaaatcat tggttaactg gctatctaac
     6781 gccagcagtt tcccggaagc gttgttgaaa ctttgtgagc ttaccagcag ggatttttgc
     6841 gcttcattca gcttcgtgat gccgtcatcc agtacct
//
LOCUS       DADMKF010000187         6774 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.190, whole genome shotgun sequence.
ACCESSION   DADMKF010000187 DADMKF010000000
VERSION     DADMKF010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6774
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.190"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(281..424)
                     /locus_tag="JNC24_003643"
     CDS             complement(281..424)
                     /locus_tag="JNC24_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_072020761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="HAY9160299.1"
                     /translation="MKKITWIILLLLAAIILAACQANYIRDVQGGTVSPSSSAELTGL
                     ATQ"
     gene            complement(522..773)
                     /locus_tag="JNC24_003644"
     CDS             complement(522..773)
                     /locus_tag="JNC24_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012644888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E5 immunity protein"
                     /protein_id="HAY9160300.1"
                     /translation="MKLSPKAAIEVCNEAAKKGLWILGIDGGHWLNPGFRIDSSASWT
                     YDMPEEYKSKIPENNRLAIENIKDDIENGYTAFIITLKM"
     gene            complement(841..2511)
                     /locus_tag="JNC24_003645"
     CDS             complement(841..2511)
                     /locus_tag="JNC24_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin"
                     /protein_id="HAY9160301.1"
                     /translation="MSGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENN
                     PWGGGSGSGIHWGGGSGHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGG
                     LAVSISAGALSAAIADIMAALKGPFKFGLWGVALYGVLPSQIAKDDPNMMSKIVTSLP
                     ADDITESPVSSLPLDKATVNVNVRVVDDVKDERQNISVVSGVPMSVPVVDAKPTERPG
                     VFTASIPGAPVLNISVNNSTPAVQTLSPGVTNNTDKDVRPAGFTQGGNTRDAVIRFPK
                     DSGHNAVYVSVSDVLSPDQVKQRQDEENRRQQEWDATHPVEAAERNYERARAELNQAN
                     EDVARNQERQAKAVQVYNSRKSELDAANKTLADAIAEIKQFERFAHDPMAGGHRMWQM
                     AGLKAQRAQTDVNNKQAAFDAAAKEKADADAALITAMESRKKKEDNKRDAEGKLNDEL
                     AKNKGKIPGLKIDQKIRGQMPERGWTEDDIKNTVSNGATGTSFDKRSPKKTPPDYLGR
                     NDPATVYGSPGKYVVVNDRTGEVTQISDKTDPGWVDDSRIQWGNKNDQ"
     gene            complement(2744..4963)
                     /locus_tag="JNC24_003646"
     CDS             complement(2744..4963)
                     /locus_tag="JNC24_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="HAY9160302.1"
                     /translation="MAIYHLSMKIISRKNGYSAVASAAYRSGSVIPDDRTGLIHDYTR
                     KRGVDDAVILTPANAPSWCGDRSVLWNAVEKAEQRRNSQLAREIELAIPREISREAAR
                     ETVLAFVRENFVSRGMIADVAFHHMDRTNPHAHIMLTTRAVGETGFAGKVRDWNDRAL
                     AETWRASWADHANRALANAGYQEEIDHRSYERQGLEKAPGLHLGKAACAMEKRGMETE
                     RGEQNRLINSLNLEIQVSRTQLALRTVQEAQRKRELSDAARRAAEALNLTIPAANASA
                     DTLREFIATLPQECGNAWEMTPEFLAMSGKVNDIEREGNALLKEQAILEKEMTGLKKA
                     RPVASLLSEIPLMTWAEPEYRKRQLRFWKLGKQIESLRRTYRAVKERDIPARRQAFET
                     QWNTWIAPGMAELKEKLSAREAERRREEAEAEARRKEQEHEARLKRHDNHRLSRETAL
                     VGVITELGRAREPGTGRITRYMMLSNRAGEFTVWGDELAHYPQSVHDPVNVYLSPGGA
                     VMVSDIREGMPESHETMARPERVRMYSGATVRHVLEQMRQGWPSYGFPALPHHWPDNF
                     YFSDDRRPVASPLPSAHRVDITADTVPEQLMPVVFSREGNSRTLNLLLCKGPEEMLVG
                     FVRREGGFCPVLVLPSPDYSHLIVSTITENGVCLAGYGEAINHDEDTPYPPEPHLMQF
                     RLKGHHDRLLAAVHKPEEMPDYLFRQLGFNQTWHEWKRDEQHRQQQRRPGHFRGMSM"
     gene            5142..5408
                     /locus_tag="JNC24_003647"
     CDS             5142..5408
                     /locus_tag="JNC24_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="HAY9160303.1"
                     /translation="MSRTLEQKIAEAEARLQRLKAKSRSLDTAQKVIVGAAMLARVRR
                     PEEAQLRAFLLQFLRKEVTRQADVNRIQPLINELEKLPKPPAKP"
     gene            complement(5633..6526)
                     /locus_tag="JNC24_003648"
     CDS             complement(5633..6526)
                     /locus_tag="JNC24_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication protein"
                     /protein_id="HAY9160304.1"
                     /translation="MSAVLQRFREKLPHKPYCTNDFAYGVRILPKNIAILARFIQQNQ
                     PHALYWLPFDVDRTGASIDWSDRNCPAPNITVKNPRNGHAHLLYALALPVRTAPDASA
                     SALRYAAAIERALCEKLGADVNYSGLICKNPCHPEWQEVEWREEPYTLDELADYLDLS
                     ASARRSVDKNYGLGRNYHLFEKVRKWAYRAIRQGWPVFSQWLDAVIQRVEMYNASLPV
                     PLSPAECRAIGKSIAKYTHRKFSPEGFSAVQAARGRKGGTKSKRAAVPTSARSLKPWE
                     ALGISRATYYRKLKCDPDLAK"
BASE COUNT         1600 a         1870 c         1563 g         1741 t
ORIGIN      
        1 aaaaaaaaca aaaagacaag gaaacaccag ggtaaacata aaaaatgcgt cagaatcgtt
       61 tttagcgcgt tgcataacta tgcacgaaag cgatgcacga tcggtttcgt gcgttctgaa
      121 caccgaacgg ctcaaaacga caagttttct ccccctggat ggaaagttct gcacggggat
      181 tttagatccc atctcccaca tggaattttt gtgacacttc aaaaaaaagc gcgtcagaat
      241 cagcctgaca cgctttgttc ttgtcaaaga ggatttcggg ttactgcgtt gctaatccgg
      301 tcagttcagc tgaggatgac ggggatacag tcccgccctg aacatcgcgg atatagtttg
      361 cctgacatgc agcaagaata atggctgcaa gaagcaataa aataatccat gttatttttt
      421 tcatactgtt ccttgtatat atggactcag gcattcatcc ataaaaacct gaaatccaaa
      481 ggttgaaatc atccatgaaa cataaaattc tataacacta tttacatctt taacgtgata
      541 atgaaagcag tgtatccatt ctcaatatca tctttaatat tttcaatagc caatctatta
      601 ttttcaggga tttttgattt gtattcctcc ggcatatcat atgtccatga tgctgaacta
      661 tctatcctga atccaggatt cagccaatgc ccaccatcaa tgcccaaaat ccataagcct
      721 tttttcgctg cttcattaca aacttctatt gcagcttttg gtgataactt catagaagca
      781 tttcctttta attcaaaaaa ggcatcacca ctatcatata acaccgtatg ttcaaataat
      841 ttattggtca tttttatttc cccattgaat tctcgaatcg tccacccaac ccggatctgt
      901 cttatcacta atctgagtaa cctcaccagt acgatcattg acaacaacat atttaccagg
      961 agaaccatac actgttgctg gatcatttcg acctaaatag tctggaggtg ttttcttcgg
     1021 actacgctta tcaaaagatg taccagttgc accgttagat acggtatttt tgatatcatc
     1081 ctctgtccat cctctttctg gcatttgtcc cctaattttc tgatctattt ttagacctgg
     1141 tatctttcct ttattttttg ctaactcatc atttagtttg ccttcagcat ctcttttatt
     1201 atcctctttc ttcttcctgc tttccatcgc agtaattaat gcagcatcag catctgcctt
     1261 ctcttttgca gcagcatcaa atgcagcctg cttattattt acatccgtct gcgcccgctg
     1321 agctttaagc ccggccattt gccacattct gtgaccgcca gccattgggt catgtgcaaa
     1381 tcgttcaaat tgttttattt cagctattgc atcagcaaga gttttattcg ctgcatcaag
     1441 ttcgctttta cgcgaattat aaacctgaac agctttagcc tgtcgctcct gatttctggc
     1501 aacatcttca tttgcctgat tcagctctgc acgcgcgcgt tcataatttc gctcagccgc
     1561 ttcaaccgga tgcgtagcat cccattcctg ctgacggcga ttttcttcat cctgacgttg
     1621 ttttacctgg tcagggctaa gaacatcact cactgaaaca tatacggcat tatgaccgct
     1681 gtccttcggg aatcgaataa ctgcatccct ggtattaccc ccctgagtaa atcctgccgg
     1741 gcgaacatcc ttatcagtat tatttgtaac acctgggctt aatgtctgta ctgctggcgt
     1801 actgttatta actgaaatat tcagaacagg tgcacctgga attgatgccg taaaaacacc
     1861 tggacgttcg gtaggttttg catcaaccac cggaacactc atcggaacac ctgaaacaac
     1921 cgaaatattc tgtcgttcgt cttttacatc atcaacaaca cgaacattta cgtttactgt
     1981 tgccttatcg agaggtaatg aactgacagg tgattcagta atatcatctg cgggtaatga
     2041 cgtcacaatc tttgacatca tattggggtc atctttcgct atttgtgatg gcaatacacc
     2101 atataaagcc accccccaaa gaccaaattt aaacggtcct ttcagggcag ccataatatc
     2161 agcaatagct gccgataatg ctcccgctga aatactgacc gccagaccgc cagctcctgg
     2221 agtggaaagt gccggaaaac caaatgccac tggcgcagct actgctgaca gattaccgcc
     2281 tgttcccgag ccaccaccgg aattaccatt ccccccgcca ttaccatgac cggaaccacc
     2341 accccagtga atgccgctac cggaaccacc accccacggg ttattttccg aactccatcc
     2401 ggagccatca gaagcaccac cacctacacc aagcccggtc gggccaccat taatgttacc
     2461 acttgtgcta tgcgcgcccg tgttatggcc gcgtccatcg ccaccgctca taaaaactcc
     2521 ctcttttaag cgttaaaaca attaattaca tgattaatac tgtacataaa accactggtt
     2581 ttatgtacag atcaaaaagt acccgtcatc gttttttctg tcaagttcag gtggaaaatt
     2641 ttttagaaat caggagggat aacacttaga aaacgcagag cgggccacaa aaaattttgt
     2701 ggcccgtgat gtgttggcgg ggtccggggt gcagccccgg atatcacata ctcatgcccc
     2761 ggaagtgccc ggggcggcgt tgttgttgcc tgtgctgttc gtcccgcttc cactcatgcc
     2821 aggtctgatt aaaaccgagt tgacggaaga gataatccgg catctcttcc ggtttgtgga
     2881 cagcagccaa aagcctgtca tgatggcctt tgagccggaa ctgcatcagg tgcggttccg
     2941 gtgggtacgg agtatcctca tcatggttta tagcttctcc gtaacctgcc aggcataccc
     3001 cgttctccgt gatggtgctg actatcagat gactgtaatc cggcgacgga agcacaagaa
     3061 cggggcaaaa ccctccttcc cggcgaacaa acccgacaag catttcctcc ggccctttgc
     3121 ataacaacag attcagcgtc ctgctgtttc cctctctcga aaatacaaca ggcatgagtt
     3181 gttccggtac agtatcagcg gtgatatcca cccggtgcgc tgacggcagc ggagaggcta
     3241 cgggcctgcg gtcgtcgctg aaataaaaat tatccggcca gtgatgcggc agcgccggaa
     3301 aaccgtaaga gggccacccc tggcgcatct gttccagtac atgccggacc gtcgcaccgg
     3361 aatacattct cacacgctca ggccgcgcca tcgtctcatg agattctggc attccctcgc
     3421 gtatatccga gaccatcaca gccccgcctg gcgacaggta aacattcacc gggtcatgaa
     3481 cactctgggg gtaatgcgcc agctcatcac cccataccgt gaattctccg gctctgttac
     3541 tcaacatcat gtagcgggtt atcctgcccg ttcccggctc tctggcacgc cccagctccg
     3601 taataacccc gactaatgcc gtttcacggc tcagacggtg gttatcatga cgtttcagcc
     3661 gcgcctcatg ctcctgttcc ttccggcgcg cttccgcttc ggcttcctcc ctgcgccgct
     3721 ccgcttcccg tgctgacagt ttttctttca gctctgccat tcccggcgca atccacgtat
     3781 tccactgcgt ttcaaaggcc tgacgacggg cgggaatgtc ccgttctttc acggccctgt
     3841 aggtgcggcg aagagattca atctgtttcc cgagtttcca gaaacggagt tgtcttttgc
     3901 ggtattccgg ttcagcccat gtcatcaggg gaatctctga cagaagggac gcgacagggc
     3961 gtgctttttt gagtccggtc atctcctttt cgagaatggc ctgctctttc agcagcgcat
     4021 tcccctcacg ttcgatgtcg ttcaccttcc cgctcatcgc caggaactcc ggggtcatct
     4081 cccacgcgtt cccgcattcc tgcggcagcg tggcaatgaa ttcccgcagg gtatccgctg
     4141 aggcattcgc agcgggaatg gtcaggttaa gggcttccgc tgcacgacgt gcagcatcgc
     4201 tgagttcccg cttacgctgc gcttcctgaa ccgtcctgag agcaagctgc gtgcgggaaa
     4261 cctgtatttc caggttaagg ctgttaatca gacggttctg ctcaccgcgt tctgtttcca
     4321 ttccccgttt ttccatcgca caggcagcct ttccgaggtg aaggcccggc gctttctcca
     4381 gtccctgacg ctcgtatgaa cgatggtcta tctcttcctg gtagccggcg ttcgcaagcg
     4441 ctctgttcgc atggtcagcc catgatgcgc gccacgtctc ggcgagtgcc cggtcgttcc
     4501 agtccctgac ctttcctgcg aatcccgttt ccccgacagc tctcgtggtc agcatgatgt
     4561 gcgcatgggg attggtccgg tccatgtgat ggaacgccac atcggcaatc atgccccgac
     4621 tgacaaagtt ttcccggaca aaagcgagaa cggtctcccg tgcggcctcg cgggaaatct
     4681 cacggggaat ggcaagttca atctcccttg ccagctggga gttccggcgc tgttcggctt
     4741 tctcgaccgc attccagaga acggaacggt caccacacca ggacggtgca ttcgcagggg
     4801 tgagaatgac cgcatcatca acgccgcgtt tacgggtgta atcgtggatt aatccggtac
     4861 ggtcatcggg tatgacagag ccggaacggt aggcagcaga agcaacagca ctgtagccgt
     4921 tttttcgcga aatgattttc atgctgagat gataaatcgc catttctgcc gtaatgcctt
     4981 tttgccgttt tcttttagcg tcgcagaccg cacacaatcg aaacgaagtg gagattgtat
     5041 aagtgcgcac ttcgtgatgt gccgtgcatt atgccagttc agttttgcaa ctaccagtga
     5101 cgcgctaaaa tgcgattttt caccgcgcat ggagtcacaa aatgagcaga acactcgaac
     5161 agaagattgc cgaagctgag gccagattac agcgcctgaa ggcgaagagc cggagcctgg
     5221 acacggcgca aaaagtcatt gtgggggctg ccatgctggc gagggtacgg aggccggaag
     5281 aggcgcaact gcgtgcgttc ctgctccagt tcctgcgaaa ggaagtcaca agacaggccg
     5341 atgtgaaccg gatacaaccg ctgattaacg agctggagaa gctacccaaa ccacctgcga
     5401 aaccgtagaa cggagaaacc ccggcgcgta agtgcccatg caccaccgga cgcctttgcg
     5461 cttcctcgct cattcgctcg ctacgctcgg tcatgagact gcggcgagcg ctaccacctc
     5521 aatcatgcgg aaagccacgc cattacgggg cagaacggag gaaatcgcaa gaaagatcac
     5581 tgctcaaaaa gacgccaaaa gggcgctgtt atctgataag gcttatctgg tctcattttg
     5641 cgagatctgg atcacatttt aattttcggt agtacgtcgc tcgactgatc cctaatgcct
     5701 cccacggctt cagcgaacgt gctgatgtag gaactgctgc gcgcttagat ttagttccac
     5761 ccttgcgacc tcgagcggcc tgtacagcgg aaaatccctc tggtgagaat ttcctgtgtg
     5821 tatatttcgc aatgctcttg ccaatagccc gacattcagc cggagaaagc ggaacgggaa
     5881 gcgatgcgtt gtacatttcg acacgctgga tcacggcatc aagccattgt gagaatacag
     5941 gccagccctg acgaatcgct ctgtaggccc atttacggac cttttcgaac agatggtagt
     6001 ttcgccccag cccgtaattt ttatcgacgc tacggcgcgc tgaggcgctc aaatcgagat
     6061 aatcagccag ttcgtcgaga gtgtagggtt cctcgcgcca ttccacttcc tgccattcag
     6121 ggtggcacgg atttttgcag atcaggccgc tgtaattcac atccgcgccc agtttttcac
     6181 acaacgcacg ctcaatagcg gcagcgtatc tgagcgccga agccgatgca tccggcgcag
     6241 ttctcacagg aagggcgagc gcgtagagca gatgcgcgtg cccgttacgg ggatttttta
     6301 cggtgatgtt aggggccgga caattccggt cgctccagtc gattgatgcc cctgtccggt
     6361 ccacgtcaaa gggaagccag tacagtgcat gtggctggtt ctgctggatg aaacgggcaa
     6421 gaatggcaat gtttttcggc agaatgcgaa cgccgtacgc gaaatcgttc gtacagtacg
     6481 gtttgtgcgg taatttttcc ctgaagcgtt gaagtacggc gctcatgtga tacgatttct
     6541 ctcggtacac agtttaacta accgcgcttt cccatcggga aacgcggaac ccggcgccct
     6601 gtaatttcgt gatcaatctt ggcggtgaga gcacgattta cggggcgttt gtttttcagt
     6661 cgcttacact acacccaacg gcaaatcctg tcgatccttc ccgaaaaacc gatccattta
     6721 ccataactcc cgttgtacgt accccttaaa acaccctcaa tcgctttctg acgc
//
LOCUS       DADMKF010000188         6772 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.191, whole genome shotgun sequence.
ACCESSION   DADMKF010000188 DADMKF010000000
VERSION     DADMKF010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6772
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.191"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(221..706)
                     /gene="sixA"
                     /locus_tag="JNC24_003649"
     CDS             complement(221..706)
                     /gene="sixA"
                     /locus_tag="JNC24_003649"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HAY9160305.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
     gene            complement(909..3053)
                     /gene="fadJ"
                     /locus_tag="JNC24_003650"
     CDS             complement(909..3053)
                     /gene="fadJ"
                     /locus_tag="JNC24_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HAY9160306.1"
                     /translation="MEMASAFTLNVRLDNIAIITIDVPDEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFISAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEAAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTDVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHAARPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            complement(3053..4363)
                     /gene="fadI"
                     /locus_tag="JNC24_003651"
     CDS             complement(3053..4363)
                     /gene="fadI"
                     /locus_tag="JNC24_003651"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HAY9160307.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLF
                     SRLRLCDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SELTLIDMHEAFAAQTLANIQLLGSERFAREVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            complement(4543..4827)
                     /locus_tag="JNC24_003652"
     CDS             complement(4543..4827)
                     /locus_tag="JNC24_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 family protein"
                     /protein_id="HAY9160308.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKARSVESEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            5199..6539
                     /gene="fadL"
                     /locus_tag="JNC24_003653"
     CDS             5199..6539
                     /gene="fadL"
                     /locus_tag="JNC24_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HAY9160309.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAVYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
BASE COUNT         1687 a         1816 c         1619 g         1650 t
ORIGIN      
        1 aataaatgac aatgagtgtc aaacagacat tattcatttc aacatgtaac tttgggcaaa
       61 atcaagatac gcgcagaaga ataatttccg aatgaaagta gaaagtgttt tatttataga
      121 tggggagagt cttcgatagt agtgagattg acagatatcc gctgcctgat gagcaattat
      181 gcccaatgaa tctacctcat tgggcataat ttggaactca tcagatagct tttgccatct
      241 tcagattaca cgggctcatc tgccagttga atgtgccgtt tccgctttca tcaagcgtta
      301 cgctggcaat ggccgaggtg gtaaacatag gcggcgtttc gcccggacat aactcggcaa
      361 ccagataccc gactaacggt aagtgagaga tcaccagtac cgaagcaaca ccttcattgg
      421 tcaacgcctg caaataggca ctgaccagac caacatcgcc gcagggcgtt aactccggca
      481 gaacttctgc actggaaggc aggttcagac aatcccctac ttcttccagt gtttgctcgg
      541 ctcgcaggaa cgggctcacc agaacgcgtt cgatttccac tttttgacct ttcagccagt
      601 tcgccatcag gcgagattcg tcacaaccat tagtggtcag aggacgaacc gaatcactgg
      661 cggcatcaag ggctgcgtcg ccgtgacgca tgataaaaac ttgcatattg caccgctttt
      721 gttaaccaga ttcgtccgtt ttctttcatc tgaaaccaga gaaagaaaac ggtggccggc
      781 attgtgcctt atccattcac cgaatgaaac gctgtttttt acctcaatgg cgtaagtata
      841 gtcaatatgc gtttacattt tgaccaacac tccgccattc agcgcggatt catatagctt
      901 tgaccttctt attgaaggtc agttgcagtt gttttccaaa aactttcccc acgcgcgccc
      961 atctcgacca aacgctcgca aggggtaaaa cgggaaccat actgcgtggc cagtcgttgc
     1021 attattgcaa ccacttcgcc cgcgccgaga gaatcgatat agcggaacgg tccaccgaga
     1081 aatggcggaa aaccaatgcc aaataccgcg ccaatatccc cgtcacgcac gctacggata
     1141 acctgctcat caacacaacg tactgcttca ttcagcatca acatcacaca ccgttcagca
     1201 acctgcggtg cggagagtcg cccctgccct tgtgcgccaa tgagcgggta aatggcggga
     1261 tcgacctgtt ttttgctttt acgccctttc tgaccataaa gatagaaacc ccgaccattt
     1321 tttctgcctt tgcgatcgtc gttcaaaatt gaagaaacaa catttgcagg cgcgctaaaa
     1381 cgttctccat aagcggcttc cagtacagga ataattttag tcccggtgtc gattcctacc
     1441 tcatccaaaa gttggattgg gcctaccgga aaaccaaatt tcactagcgc ggcatcaatg
     1501 tgctctaccc gttcaccttc ggtcagcatg cggatagctt cattaatgta aggcgctaag
     1561 atgcgattga cgtaaaaccc ggctttgtca cgcacgacaa tgggcgtttt gccctgtttt
     1621 ttcgccagtt ttaccgtagt ggcgatggtt tgcgccgatg tacccgcatg aggaataatc
     1681 tctaccagcg gcattttttc cacaggactg aagaaatgca ggccaataac ttgctcaggt
     1741 cgcgcggcgt gagcggcgat atcaccaatc ggtaaagatg acgtgttcga agcaaaaatg
     1801 gtatgagcgg cgcaattttg ctccacttcc gccaccatct gttgttttaa ttcgagattt
     1861 tcaaacaccg cttcaataat cagatcgcga tgggcaaagc cgcgatagtc cgtcgttccg
     1921 gagattaatg ccagctgttt gtcacgttcg ctggctttga gatgacgacg gcgaactttg
     1981 ccctccagct gatcccaact gtacttcagc gcatgattta tgccctgcgg gttgatatct
     2041 ttaattctga ccggaagccc cgctttacaa gcagtgacat aagcaatacc gccgcccatc
     2101 aagccaccac ctaaaattcc cacgctgttt aatggcgcag gcggtgcatc actaccggga
     2161 tctttcttca cgtccgtact ggcaaaaaag atactacgca aagcttgcga ttgcggcgtc
     2221 atcgccagtt cgccaaaggc gcgagcttca gcgtcatagc cgctgctggt gccctgcgct
     2281 aatcccgttt caacaacctc caggatgcgt tctgtcgccg gataattgcc ttgagttttg
     2341 tgttctgttt tcttgccgac cattttgaac agcagcgcac gacctaacgg ccccgccaga
     2401 atacgctcgc gtacaggtag agggcgggaa gatgggcgat ccttcttcgc cagctcaaca
     2461 gcggcttcca gcaaaatgga gtgcggaaca acgtcatcca ccagccccag ctttaatgcc
     2521 tgtttcgccc gaagttgttt tccggtgaga atcatctcta atgctgtact gacgcctatc
     2581 agacgcggta aacgctgggt gccgcctgaa ccgggtaaca atccaagttg tacttcaggc
     2641 aaaccgagca ccgttttagg atcgtcagta caaacgcgac cgtggcacgc cagcgccagc
     2701 tccagcccac cgcccaggca agcgccatga atagccgcga taaccggaat gggcaaagca
     2761 tgaatctccg ccatcaactg ttggccctgc cgcgccagtg cttccgcttc ttgcgccgtt
     2821 ttgcagttgc caatcatgtt gatgtctgct ccggcgataa aattgtccgg tttcgcggag
     2881 ataaacacca cgccgcgcaa ctctttgttt tcacggagtt gcttaataat ggcgcgcacc
     2941 tgcgaggcaa attccgcctt cagggtattc attttctcat ccggtacatc gatggtgatg
     3001 atagcaatgt tgtccagacg aacattaaga gtaaacgctg atgccatttc cattattccg
     3061 cctccagaac cattgccgcg ccaagcccac cggcagcaca ggcggtaact aaaccaaatc
     3121 caccgccgcg acggcgaagt tcatgcaatg tctgggtaat catccgcgcg ccggtcgccg
     3181 cgaagggatg cccgtaagca atcgaaccgc caagcacgtt aaatttgcta tcgtccactt
     3241 cgccagtggc atgtgcacgc cccagtactt cacgagcaaa acgttcacta cccagcaact
     3301 gaatattcgc cagtgtctga gctgcaaagg cttcgtgcat atcgatcaat gtcagttcgc
     3361 tcatcgtcaa accggcacgt tccagcgcca gcggtgttga ccaggctgga ccgagcaaca
     3421 tgtcctgcca gacatcaatc gcagtaaatg cgtagctgcg cagatacccc agcggcacca
     3481 gccctaattc tttcgcccgg gattcagtca tcaggatcac cgctgccgcg ccatcggtca
     3541 gcggcgtact gtttgccgcc gttaccgttc cgtgtttgcg atcaaacgcc gggcgcagct
     3601 ttgcgtaatc ggcaagcgag gaattaccgc gaatattgtt gtcttcgaca agcggttgtt
     3661 tataaggagg gataaaggca gtcatcacct cttctttgag ttttccgtct gaccatgcct
     3721 gagcggcacg ctgatgcgaa cggtgcgcta atgcatcttg ctgttctcgg gtgatgccgt
     3781 aggttttcgc catttgttcg gcggtgtcgc ccatccgcaa gccagtagaa tattctgcta
     3841 ccgcaggcgg tacgggcatt aagtcgcaca aacgcaggcg agagaagagt ttcaggcgct
     3901 ggctcatggt tcgagctttg ttgacatcaa ccagcacgcg cgccagtttt ttactgacgc
     3961 caattggcaa tacggaagag gaatctgccc caccggcaat ccccgctcgg atagttcccg
     4021 ccatcaggct ttctgcgacg tttgcaaccg cctggaaact ggtagcgcaa gcgcggctga
     4081 cgctgtaagc atcggtatgc acattcattc cggtaccgag aacaatttca cgcgcaatgt
     4141 tgggggcttc aggcatttgt acgacctgac caaagaccag ctgttcaatc acttcagcgg
     4201 ggatctcgct gcgtgccagc agttcgccta ccaccatctt ccctaaatca accgcgggaa
     4261 tgccatgaaa agccgtcgcc tggcgggcaa aaggcgtacg taaaccgcta acaatggcga
     4321 tacgatcgcc ctggcgggta accagcggta aaacctgacc cataacactc ccctgtaaaa
     4381 aaataaataa agtggtctga cctgatcata gtcttaacta tttttttaca tttagccaag
     4441 cggagaaaaa ggaaagtggg agctatgaca cagtgaagac aggagaaaga aaagaaaacg
     4501 cccctgccag caactgacag gggcgaacgt acccgagagg aattaacgca gacccagctg
     4561 gaagatcagc atttcagctt cgcaggcaaa agtgaaatcg atatccaggc gcacaccgtc
     4621 agactcttca gtgaaagtcg gggagatttt gcacggttcg gattccacgc tacgggcttt
     4681 ttcagtcagc gccgccagcg tttgttctgc ttctgcgcgg tttgcaaaca cgcggctgta
     4741 agacgcggtg cagtcggagt tgtccataat ggtgccaaca tccatacagc agcaaaccgg
     4801 ggtttcatca gcactacatt tactcatcgt tgatttcctc tgtatgtgca cccaaggtgc
     4861 cagataaacg ttgtggatat tttacgcttc cggaaagtgc tgctccagtt gttaattctg
     4921 caaaatcgga taagtgaccg aaatcacact taaaaatgat ctaaaacaaa attcacccga
     4981 atccgcgagt gcgcaacctc caaattttgc cagctggatc gcgtttctta gatcatattt
     5041 gaaaaaagat ggaaacatac ttgcagcatt ccagctggtc cgacctatac tctcgccact
     5101 ggtctgattt ctaagatgta cctcagaccc tacacttcgc gctcctgtta cagcacgtaa
     5161 catagtttgt ataaaaataa atcattgagg ttatggtcat gagccagaaa accctgttta
     5221 caaagtctgc tctcgcagtc gcagtggcac ttatctccac ccaggcctgg tcggcaggct
     5281 ttcagttaaa cgaattttct tcctctggcc tgggccgggc ttattcaggg gaaggcgcaa
     5341 ttgccgatga tgcaggtaac gttagccgta accccgcatt gattaccatg tttgaccgcc
     5401 cgacattttc tgcgggtgcg gtttatattg acccggatgt gaatatcagc ggaacgtctc
     5461 cgtctggtcg tagcctgaaa gccgataaca tcgcgcctac ggcatgggtt ccgaacatgc
     5521 actttgttgc accgattaac gaccaatttg gttggggcgc ttctattacc tctaactatg
     5581 gtctggcaac agagtttaat gatacttatg caggcggctc tgtcgggggt acaactgacc
     5641 ttgaaaccat gaacctgaac ttaagcggtg cgtatcgctt aaataatgca tggagctttg
     5701 gtcttggttt caacgccgtc tacgctcgcg cgaaaattga acgtttcgca ggcgatctgg
     5761 ggcagttggt tgctggtcag attatgcaat ctcctgccgg gcaaactcct caagggcaag
     5821 cattggcagc taccgccaac ggtatcgaca gtaataccaa aatcgctcat ctgaacggta
     5881 accagtgggg ctttggctgg aacgccggaa tcctgtatga actggataaa aataaccgct
     5941 atgcactgac ctaccgttct gaagtgaaaa ttgacttcaa aggtaactac agcagcgatc
     6001 ttaatcgtgc gtttaataac tacggtttgc caattcctac cgcgacaggt ggcgcaacgc
     6061 aatcgggtta tctgacgctg aacctgcctg aaatgtggga agtgtcaggt tataaccgtg
     6121 ttgatccaca gtgggcgatt cactatagcc tggcttacac cagctggagt cagttccagc
     6181 agctgaaagc gacctcaacc agtggcgaca cgctgttcca gaaacatgaa ggctttaaag
     6241 atgcttaccg catcgcgttg ggtaccactt attactacga tgataactgg accttccgta
     6301 ccggtatcgc ctttgatgac agcccagttc cggctcagaa tcgttctatc tccattccgg
     6361 accaggaccg tttctggctg agtgcaggta cgacttacgc atttaataaa gatgcttcag
     6421 tcgacgttgg tgtttcttat atgcacggtc agagcgtgaa aattaacgaa ggcccatacc
     6481 agttcgagtc tgaaggtaaa gcctggctgt tcggtactaa ctttaactac gcgttctgat
     6541 aacgcgttcg cctggataaa gtcacctgca tagcaggtga ctttaactcc cccacttcac
     6601 cgaaagtagt gtcctcattg cttacctcct gagttttgca aacagcctgt tggcagcttg
     6661 ccctttcaaa tcaataagcg gtatccacag acaaacctga agggaaaggc atattttcag
     6721 gcgttctgct cgtccttctc aaagagttta cttttctgca tttccaggat ac
//
LOCUS       DADMKF010000189         6712 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.193, whole genome shotgun sequence.
ACCESSION   DADMKF010000189 DADMKF010000000
VERSION     DADMKF010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6712
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.193"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            84..758
                     /locus_tag="JNC24_003654"
     CDS             84..758
                     /locus_tag="JNC24_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAY9160310.1"
                     /translation="MSDNLLNKLTQLKLPAMAGSLIRQRETPQTYDELSFEERLTLLV
                     DDELLSRENSRVARLRKNACLKYQATPEGLRYPASRGLRAEQMRELLNGYYIIHRKNL
                     LITGPTGCGKSWIANALGEQACRQKYSVRYCRTGRLLEQLAQGRVDGSWLKYLKQLQK
                     IQVLISSTIVVSQFPVDKWHGLMENPTTADAILDRLVHNSHRVVLQGESLRKNPPTVE
                     SSEKTS"
     gene            complement(897..1259)
                     /locus_tag="JNC24_003655"
     CDS             complement(897..1259)
                     /locus_tag="JNC24_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HAY9160311.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATDPEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            1796..3709
                     /locus_tag="JNC24_003656"
     CDS             1796..3709
                     /locus_tag="JNC24_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HAY9160312.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGERGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGTSPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            3939..5087
                     /gene="mtlD"
                     /locus_tag="JNC24_003657"
     CDS             3939..5087
                     /gene="mtlD"
                     /locus_tag="JNC24_003657"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HAY9160313.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISDLDANSEVVSEAVTAYKAMQ"
     gene            5087..5674
                     /gene="mtlR"
                     /locus_tag="JNC24_003658"
     CDS             5087..5674
                     /gene="mtlR"
                     /locus_tag="JNC24_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor MtlR"
                     /protein_id="HAY9160314.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISRISLKKAFQK"
     gene            complement(5686..5895)
                     /locus_tag="JNC24_003659"
     CDS             complement(5686..5895)
                     /locus_tag="JNC24_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9160315.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            6180..6542
                     /locus_tag="JNC24_003660"
     CDS             6180..6542
                     /locus_tag="JNC24_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HAY9160316.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLQEMTGFSKTAF"
BASE COUNT         1741 a         1644 c         1758 g         1569 t
ORIGIN      
        1 gggcgaacaa catccggcat atgcttcaga gcatgaaaac ctgcgtggtc ctggctacta
       61 ccactgacag aaggaacaac cgtatgagtg ataatctgtt aaacaaactg acccaactga
      121 aactcccggc gatggccggg agcctgatac ggcaacggga aacgcctcag acttacgacg
      181 aactgtcgtt cgaggaaagg ctgacactgc tggttgatga cgagcttctg agcagagaaa
      241 acagtcgtgt ggcacggttg agaaaaaatg cctgcctgaa atatcaggca acaccggaag
      301 ggctgcgtta tccggcctca agaggtctgc gggctgaaca gatgagggaa ctgctgaacg
      361 gctactacat aatccacagg aaaaacctac tgataaccgg tccgacagga tgcggtaaga
      421 gctggatagc caatgcgctg ggcgaacagg catgtcgaca gaaatacagc gtccggtact
      481 gtagaaccgg gcgattactg gaacagttgg ctcagggacg tgttgatggc agttggctga
      541 aatatctgaa acagttgcaa aaaatacagg ttctaataag cagcacgata gtggtaagcc
      601 agttcccggt agataaatgg catggtctga tggagaaccc aacaacagca gatgccatcc
      661 tggacagact ggtacataac tcacacagag tggtccttca gggagagtcc ctgaggaaaa
      721 atcccccgac agtggaaagc agcgaaaaaa cgagttaaaa agcggtagaa aaagttaagt
      781 tcccggttga tcgaatggat gcgaatcgct gatcgaactt cgatgccaat ccgtgatcga
      841 atagagcgga atacgcacca gcaccacgta agttaaaaca atcaatagat ccataattag
      901 atctccggga acgtggaata gtcagtcttt ttctcggcgg cgagttgatg ctctagccgg
      961 gaaattcggt cagaaagtgc ggcgatttcc gggtcagtcg ctttctcctg cgacgcaaca
     1021 tgtgactgca tgccccaccc gcgctccggt tgatagagcg tcgcccagat ccacaggaac
     1081 ggccagataa catgcagggt aaacaggctc acccagcccg ccgtatgaat agcgtcggca
     1141 tggggatggt tgcgcttttt ggcaatcaga taagggatgt catgtatcgc gatgattcca
     1201 taaaaaatca ccagaaagac gaagatcagc actcccaacg cgaaatagtt tagaaacata
     1261 tctgcctcgg attcacgttt atcagtgttg tttttgggct ggcagccaga agggagtcag
     1321 gctgatattt tgacaataat ccgggttcgc gattctcgcc ataacaccaa agaataattt
     1381 ttagaggtga tgagttgctt agttacataa caattgtatg acgaaggcat aacatgctgt
     1441 agatcacatc aggtgaacgc cgtaagaaaa tatcttgtga ttcagatcac aaagattcaa
     1501 caaaccatca aaacaaaaat gtgacactac tcacatttaa atgccatttt tagcgaaaat
     1561 cgccgccttg ttgctttttt acacaagcgt tttgtgatga acgtcacgtt aattacctct
     1621 ctacccccta tatttatgtg attgatatca cacaaaaggc cgtcgactgg acagttaacc
     1681 gattcagtgc cagatttcgc agtatctaca aggtccggct acctctgccg ccacattaac
     1741 aaaaaacctc gggcttccag cctgcgcgac agcaaacata agaaggggtg tttttatgtc
     1801 atccgatatt aagatcaaag tgcaaagctt tggtcgtttc ctcagcaaca tggtgatgcc
     1861 aaatatcggc gcgtttatcg cgtggggtat catcaccgcg ttatttattc caacagggtg
     1921 gttaccgaac gagacgctgg cgaagctggt cgggccgatg atcacttatc tcctgccgct
     1981 gctgatcggt tataccggtg gtaagctggt aggcggcgaa cgtggcggcg tagtcggtgc
     2041 catcaccacc atgggcgtta tcgtcggcgc agacatgccg atgttcctcg gttctatgat
     2101 tgcaggtccg ctgggcggct ggtgcattaa gcacttcgac cgctgggtag acggtaagat
     2161 caaatccggt tttgagatgc tggtgaataa cttctccgca ggcatcatcg ggatgatcct
     2221 cgctattctg gcattcctcg gcattggccc gattgttgaa gccctgtcca aaatgctggc
     2281 tgcgggcgtt aacttcatgg ttgtccatga catgctgccg ctggcgtcta tctttgttga
     2341 accggcgaaa atcctgttcc tcaacaacgc cattaaccac ggtatcttct cgccgctggg
     2401 tattcagcag tcccatgaac tgggtaaatc aatcttcttc ctgattgaag ctaacccagg
     2461 tccaggtatg ggcgtgctgc tggcgtacat gttctttggt cgtggtagcg ctaaacagtc
     2521 tgcgggcggt gcggcaatca tccacttcct gggtggtatc cacgaaatct acttcccgta
     2581 tgtgctgatg aatccgcgtc tgatcctcgc agtcatcctc ggcggtatga ctggcgtgtt
     2641 cacgctgact atcctgggcg gtggtctggt ttctccggca tctccgggtt ctatccttgc
     2701 tgtactggcg atgacaccaa aaggtgctta tttcgctaac atcgcgggtg tgtgtgcggc
     2761 gatggctgtc tccttcgttg tctctgctat tttgctgaaa accagcaaag tgaaagaaga
     2821 agatgatatt gaagcagcaa ctcgtcgtat gcaggacatg aaagctgagt ctaaaggcac
     2881 atctccgctg tctgcgggcg atgtgactaa cgacctgagc cacgtacgta aaatcatcgt
     2941 tgcctgtgac gccggtatgg gttccagtgc gatgggcgca ggcgtgctgc gtaagaaaat
     3001 tcaggatgca ggtctgtcac agatttcggt cactaacagc gcgatcaaca acctgccgcc
     3061 agatgtggac ctcgtcatca ctcaccgtga cctgaccgaa cgcgctatgc gccaggttcc
     3121 gcaggcgcag catatttcgc tgaccaactt cctcgacagc ggcctgtaca ccagcctaac
     3181 cgaacgtctg gttgctgccc agcgccatac tgaaaacgaa gtgaaagtga aagacagtct
     3241 gaaagacagc tttgacgatt ccagtgctaa cctgttcaaa ctgggcgcgg agaacatctt
     3301 cctcggtcgc aaagcagcaa ccaaagaaga agcgattcgt tttgctggcg agcagctggt
     3361 gaaaggcggt tacgttgagc cggaatacgt tcaggcgatg ctggatcgtg aaaaactgac
     3421 cccgacttat ctgggtgagt ctatcgcggt gccacacggt acggttgaag cgaaagatcg
     3481 cgtactgaaa acgggcgtgg tgttctgcca gtacccggaa ggcgtgcgct tcggtgaaga
     3541 agaagatgac attgcccgtc tggtgattgg tattgcagcc cgtaacaacg agcacattca
     3601 ggttatcacc agcctgacca atgcactgga tgatgagtcc gtcatcgagc gtctggcaca
     3661 caccaccagc gtggatgaag tgctggaact gttggcaggt cgtaagtaat ccaatcccac
     3721 cctctccaca tggagaaggt ggggttaatt gcctgatgcg ctacgcttat caggcctaca
     3781 ggatgcatca cgatttgttg aatttgcacg ttcttgtagg ccggataagg cgcttacgcc
     3841 gcatccggcg ctgcccctct cctcacggag agggtttggg tgagggaaaa gcctcacccc
     3901 agccctctcg ggtaaaaaca ttgatgaagg ttaatactat gaaagcatta cattttggcg
     3961 caggtaatat cggtcgtggc tttatcggta aactgctggc agacgcgggt atccaactga
     4021 cgtttgccga tgtcaatcag gtggtacttg atgccctgaa tgcccgtcat agctatcagg
     4081 ttcatgtggt tggtgaaacc gagcaggtag ataccgtttc cggcgtcaat gctgtcagca
     4141 gcattggtga tgatgtcgtt gatctgattg ctcaggtcga tttagtcact accgccgttg
     4201 gcccggttgt gctggaacgt attgctccgg ctatcgccaa agggctggtg aaacgcaaag
     4261 aacaaggtaa tgaatccccg ctgaacatca tcgcctgtga aaacatggta cgcggtacca
     4321 cgcagctgaa aggccatgtg atgaacgccc tgccggaaga cgccaaagcg tgggtagaag
     4381 aacacgttgg ctttgtcgat tccgccgttg accgcatcgt accgccttcg gcttcggcaa
     4441 ctaacgatcc gctggaagtg acggtagaaa ctttcagcga atggattgtc gataaaacgc
     4501 agttcaaagg cgcactgccg aacatcccag gcatggagtt aaccgacaac ctgatggcat
     4561 ttgtcgaacg taaactcttc accctgaaca cgggtcatgc tataaccgcg tacctcggaa
     4621 aactggccgg tcatcagacc attcgtgacg ctattctcga cgagaaaatc cgcgcggtgg
     4681 taaaaggtgc gatggaagaa agtggtgcgg tactgatcaa gcgctacggc tttgacgcag
     4741 acaaacatgc ggcgtacatc cagaaaatcc tcggtcgttt tgagaacccg tatctgaaag
     4801 atgatgtaga gcgcgtaggc cgtcagccgc tgcgtaaact gagtgctggc gaccgtctga
     4861 tcaagccatt gctcggtacg ctggaataca gccttccgca caagaatctg attcagggga
     4921 ttgctggtgc aatgcacttc cgcagtgaag atgatccgca ggctcaggaa ctggcagcac
     4981 tgatcgctga caaaggtccg caggcggcgc tggcacagat ttccgatctt gatgccaaca
     5041 gcgaggttgt atccgaggcg gtaaccgctt ataaagcaat gcaataatgg tggaccaggc
     5101 gcaggacacc ctgcgcccga ataacagatt gtcagatatg caggcaacaa tggaacaaac
     5161 ccaggccttt gaaaaccgtg tgcttgagcg tctgaatgct ggcaaaaccg tgcgaagctt
     5221 tctgatcacc gccgtcgagc ttttgaccga ggcggtaaat cttctggtgc ttcaggtatt
     5281 ccgcaaagac gattacgcgg tgaagtatgc tgtagaaccg ttactcgacg gcgacggtcc
     5341 gctgggcgat ctttctgtgc gtttaaaact catttacggg ttgggcgtca ttaaccgcca
     5401 ggaatacgaa gatgcggagc tgctgatggc attgcgtgaa gaactaaatc acgacggcaa
     5461 cgagtacgcc tttaccgacg acgagatcct tggacccttt ggtgaactgc actgcgtggc
     5521 ggcgttacca ccgccgccac agtttgaacc agcagactcc agtttgtatg caatgcaaat
     5581 tcagcgctat caacaggctg tgcgatcaac aatggtcctt tcactgactg agctgatttc
     5641 cagaatcagc ttaaaaaaag cctttcaaaa gtaagcaacg tctgcttact gcccctctac
     5701 ctgcttcggc cgataaagcc gacgataata ctccagacgt tgtagatata acggcacgct
     5761 ttcatcgggt attccggacg gaatcgcgtt acggggagga agttttttca gatactcccg
     5821 gaacgcctgg cttgatgcca tgaaatctac ggctttatcg ataagaagcg gaacgttttc
     5881 acctaatttg cccatgatga tatctccgta ttaccccgcg ccgggaatgc gcggccgcca
     5941 attttagttt aggttcccgg taaagatgac attaaggaaa acgtgctgaa tcctcaataa
     6001 tcatccgctg ctgtttatta tcttttttct atagatttct taattaatca ataaattgcg
     6061 tagtttttaa gcatgttaat gattacgtta cagaactatt gcaatgtcat cagttgtgaa
     6121 caaagcacct ggtcgcgcat actaggggct ataaatttat ctttatcaga agccatcaca
     6181 tgaaagaagt cgaaaaaaac gaaatcaaac gtctcagcga tcgtctggac gccatccgcc
     6241 accagcaggc cgatctgtcg ctggttgaag ccgcagacaa atatgccgag ctggaaaaag
     6301 agaaagccac gctggaagca gaaattgctc gcctgcgtga agttcatagc caaaaactga
     6361 gtaaagaagc acagaagctg atgaagatgc cgtttcagcg cgcaattacc aaaaaagagc
     6421 aggctgatat gggcaagctg aagaaaagtg ttcgcggact ggtggttgtg cacccaatga
     6481 ccgcgctggg ccgtgaaatg ggcctgcagg agatgactgg attctctaag actgcgtttt
     6541 aagcgcctca gacgactggt tcgatcatat caacccgcat ctggcttgcc ttagctggtc
     6601 aatgcgggga ctctcctgtc tactcgccta attaccaaac aattcactcc ctttacgtcg
     6661 atttaccgct ggttgatatt ctgaaaatgc atttttaccg aaatagccac ct
//
LOCUS       DADMKF010000190         6621 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.194, whole genome shotgun sequence.
ACCESSION   DADMKF010000190 DADMKF010000000
VERSION     DADMKF010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6621
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.194"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(24..605)
                     /locus_tag="JNC24_003661"
     CDS             complement(24..605)
                     /locus_tag="JNC24_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9160317.1"
                     /translation="MYYPVTDYIALALIISFLFLTLFICLLCLKHERIKKETIRQKNA
                     HILEHGWNATEFSWFRYGQYNETGIYISIEKTIIITITVSGECFKKEYSIVSHMLVTD
                     TITEATLYENGLYTRHIRLSRPVSDSKYPLPPGSQLIKNMTLRLRLQDQQEETSVTLF
                     QGKMSTDGNNYYIIKGKVSSVLLLLKMLQINHA"
     gene            complement(652..4509)
                     /gene="sigA"
                     /locus_tag="JNC24_003662"
     CDS             complement(652..4509)
                     /gene="sigA"
                     /locus_tag="JNC24_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin SigA"
                     /protein_id="HAY9160318.1"
                     /translation="MNKIYSLKYSHITGGLVAVSELTRKVSVGTSRKKVILGIILSSI
                     YGSYGETAFAAMLDINNIWTRDYLDLAQNRGEFRPGATNVQLMMKDGKIFHFPELPVP
                     DFSAVSNKGATTSIGGAYSVTATHNGTQHHAITTQSWDQTAYKASNRVSSGDFSVHRL
                     NKFVVETTGVTESADFSLSPEDAMKRYGVNYNGKEQIIGFRAGAGTTSTILNGKQYLF
                     GQNYNPDLLSASLFNLDWKNKSYIYTNRTPFKNSPIFGDSGSGSYLYDKEQQKWVFHG
                     VTSTVGFLSSTNIAWTNYSLFNNILVNNLKKNFTNTMQLDGKKQELSSIIKDKDLSVS
                     GGGELTLKQDTDLGIGGLIFDKNQTYKVYGKDKSYKGAGIDIDNNTTVEWNVKGVAGD
                     NLHKIGSGTLDVKIAQGNNLKIGNGTVILSAEKAFNKIYMAGGKGTVKINAKDALSES
                     GNGEIYFTRNGGTLDLNGYDQSFQKIAATDAGTTVTNSNVKQSTLSLTNTDAYMYHGN
                     VSGNISINHIINTTQQHNNNANLIFDGSVDIKNDISVRNAQLTLQGHATEHAIFKEGN
                     NNCPIPFLCQKDYSAAIKDQESTVNKRYNTEYKSNNQIASFSQPDWESRKFNFRKLNL
                     ENATLSIGRDANVKGHIEAKNSQIVLGNKTAYIDMFSGRNITGEGFGFRQQLRSGDSA
                     GESSFNGSLSAQNSKITVGDKSTVTMTGALSLINTDLIINKGATVTAQGKMYVDKAIE
                     LAGTLTLTGTPTENNKYSPAIYMSDGYNMTEDGATLKAQNYAWVNGNIKSDKKASILF
                     GVDQYKEDNLDKTTHTPLATGLLGGFDTSYTGGIDAPAASASMYNTLWRVNGQSALQS
                     LKTRDSLLLFSNIENSGFHTVTVNTLDATNTAVIMRADLSQSVNQSDKLIVKNQLTGS
                     NNSLSVDIQKVGNNNSGLNVDLITAPKGSNKEIFKASTQAIGFSNISPVISTKEDQEH
                     TTWTLTGYKVAENTASSGAAKSYMSGNYKAFLTEVNNLNKRMGDLRDTNGEAGAWARI
                     MSGAGSASSGYSDNYTHVQIGVDKKHELDGLDLFTGLTMTYTDSHASSNAFSGKTKSV
                     GAGLYASAIFDSGAYIDLISKYVHHDNEYSATFAGLGTKDYSSHSLYVGAEAGYRYHV
                     TEDSWIEPQAELVYGAVSGKRFDWQDRGMSVTMKDKDFNPLIGRTGVDVGKSFSGKDW
                     KVTARAGLGYQFDLFANGETVLRDASGEKRIKGEKDGRILMNVGLNAEIRDNLRFGLE
                     FEKSAFGKYNVDNAINANFRYSF"
     gene            complement(4870..5907)
                     /locus_tag="JNC24_003663"
     CDS             complement(4870..5907)
                     /locus_tag="JNC24_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001363149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
                     /protein_id="HAY9160319.1"
                     /translation="MHRGFCVAHEPSILLPSVGAQLSNPDNITDNADRISDEWKAFDR
                     AIESNHGVPEAAARLKEHLRDFRNTHAAAQLGVSAVAALPGDRLSAALMVKSHGTVSV
                     DGKVSDADLTYLEEVANSTGQEVDKSRLTSQAFARAALITDVGIALATELETAGQKWS
                     LGFPPKFQRVDLFNYNVLVRNYDSSAFKGDRYHNTKNGINADIGASTDLDDNWTLGLV
                     AQNLIPRSIETKEVNGITETFRIRPQVTAGVSWHNAMFTTAFDVDLTPASGFTSDSNR
                     QFAAIGAEFNAWKWAQLRAGYRQNLAGNDGSAFTAGVGISPFDVVHLDVAGLIGTDNT
                     YGAVAQFQFTF"
     gene            5859..6236
                     /locus_tag="JNC24_003664"
                     /pseudo
     CDS             5859..6236
                     /locus_tag="JNC24_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014839879.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(6275..>6621)
                     /locus_tag="JNC24_003665"
     CDS             complement(6275..>6621)
                     /locus_tag="JNC24_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000555401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAY9160320.1"
                     /translation="VPGEYSSGNSIRPRGITKVGNSELRRLLYEAAWSYRTPAKVGAW
                     LIYYRPDSVTQYSKDIAWKAQQRLCSRYRTLTAKGKKSQVAITAVARELTGFMWDIAL
                     AAQSSFSQQKQN"
BASE COUNT         1644 a         1471 c         1493 g         2013 t
ORIGIN      
        1 acctcaaaat accgggacag agatcaggcg tgattaatct gcagcatttt taataacaga
       61 agtactgagc ttactttacc tttgatgatg taatagttat ttccgtcggt actcattttt
      121 ccctgaaata atgtcacgga tgtttcttcc tgctgatcct gcaggcgcag tcgtaaggtc
      181 atatttttaa tgagctgaga tccgggtggc aaggggtatt tgctgtcaga tactgggcga
      241 ctgagtctga tgtggcgcgt atacagcccg ttttcgtaca gggtggcctc tgttatggtg
      301 tcagtgacca gcatgtggga gacaatgctg tattcttttt taaagcactc accagaaacg
      361 gtgattgtaa tgataatggt cttctcgatc gagatgtaaa tacccgtttc gttatactgt
      421 ccgtatcgga accatgagaa ctcagttgca ttccagccat gctccagaat atgtgcattt
      481 ttttgcctga tagtttcttt ttttattcgc tcatgtttaa ggcataacag gcagataaat
      541 aatgtcagaa aaagaaagct aataatgaga gcaagagcga tatagtctgt cacagggtaa
      601 tacatattct ctacggtaga agaagggccg caaacgcggc ccgggctgtt atcagaaaga
      661 gtaacggaag ttggcgttga tcgcgttatc cacgttgtat ttaccaaatg ccgatttctc
      721 aaactcaaga ccgaagcgaa gattatcgcg aatttcggcg ttgagaccaa cattcatgag
      781 aatacgaccg tctttttcac ctttgatacg tttctcaccg gacgcatcac gcagtacggt
      841 ttcaccgttg gcaaacaggt caaactggta gccaaggccg gcgcgggctg tgactttcca
      901 gtccttaccg gagaaggatt tacccacatc aacaccggta cgcccaatca gcggattaaa
      961 gtccttatcc ttcatggtca cgctcattcc gcgatcctgc cagtcgaacc gtttacctga
     1021 tacggcccca taaaccagtt ctgcctgcgg ctcaatccag gagtcttctg ttacatgata
     1081 gcggtagcct gcttcagcac ccacatacaa ggaatgagaa ctgtagtctt ttgttccgag
     1141 tccagcaaag gtcgccgagt actcattatc atggtgaaca tacttactaa tcaggtcgat
     1201 ataggcacca gagtcaaata tagcggaagc atacagacct gccccgacgg acttcgtctt
     1261 gccactgaat gcattactgc tggcatgact gtcggtatac gtcatagtca gaccagtgaa
     1321 aaggtcaagt ccatccagct catgtttttt atccacacca atctgcacat gggtgtagtt
     1381 gtcactgtat ccactagaag ctgaacctgc tccgctcatg atgcgggccc atgcaccggc
     1441 ctcgccattg gtgtcacgca gatcccccat tcgtttattc aggttgttga cttctgtcag
     1501 gaaggctttg taattaccgg acatatacga ttttgctgca ccggaagatg ctgtattttc
     1561 agccacctta tatccggtca gggtccacgt ggtatgttcc tgatcctctt tcgtgctgat
     1621 cacaggagat atgttgctga aacctatggc ctgagtactg gctttaaata tctctttatt
     1681 gcttcctttt ggggctgtta tcaggtcaac gtttaatcct gagttattat ttcccacttt
     1741 ctgtatatcg accgacagac tgttattgct tccggttaac tgatttttaa caatgagttt
     1801 atccgattga tttacagact ggctcagatc agcccgcata atcacagcag tattagtggc
     1861 atccagtgtg tttactgtca cagtatggaa acccgaattc tctatgttac taaacaacaa
     1921 aagactgtcg cgggttttta atgattgcag ggctgactgt ccgtttactc tccataaggt
     1981 gttatacatg ctggctgagg cagcaggagc atcaatacct ccggtataag aagtatcaaa
     2041 gccacccagc aaacctgtag ccagcggtgt gtgtgtggtt ttgtccaggt tatcttcttt
     2101 atactggtca acaccaaaca gaatagatgc ttttttgtct gattttatat taccattgac
     2161 ccaggcataa ttttgagcct ttaacgtggc accatcttct gtcatattat atccatctga
     2221 catatagatt gccgggctgt atttattatt ttctgtaggg gtgcctgtta atgtcagggt
     2281 tccggccagt tcaatagctt tatctacata catttttccc tgggcggtaa cagtagctcc
     2341 tttgttgata atcaggtctg tattaattaa ggataatgca ccagtcatag taacagttga
     2401 tttatcacca acagttattt tgctgttttg agcactcaga ctgccgttga aactactttc
     2461 gcctgctgaa tccccggagc gaagctgttg tctgaatcca aaaccttcgc cagtaatgtt
     2521 tcttcctgag aacatgtcaa tgtatgcagt tttatttccc agaacaattt gagagttttt
     2581 agcctctatg tgtcctttta cattagcatc ccggcctata ctcagggttg cgttttctaa
     2641 atttaatttc cggaaattaa atttacgact ttcccagtcg ggctgggaaa aagaggctat
     2701 ctgattgttg gacttatatt ccgtattgta acgtttattt acagtgcttt cctggtcctt
     2761 tatggcagca gaatagtctt tttgacataa aaaaggaatt ggacagttgt tattgccttc
     2821 tttaaatatg gcatgttctg tcgcatgtcc ttgtaatgtt aactgtgcat tccggacaga
     2881 gatatcgttt ttgatatcga ctgagccatc aaagatcaga ttggcattat tgttatgttg
     2941 ctgggtagta ttgataatat gatttatgct tatattacct gatacattcc catggtacat
     3001 atatgcatca gtattagtaa gtgataatgt tgattgcttc acgtttgagt tcgttacggt
     3061 tgttcccgca tctgttgctg cgattttctg aaatgactgg tcatagccgt ttagatccag
     3121 tgttccgcca tttctggtaa aatagatttc gccattaccg ctttcgctta aagcgtcttt
     3181 ggcatttatt tttaccgtac ctttacctcc ggccatgtaa attttattga aggctttttc
     3241 agcactaagg atgacagtcc cattacctat tttaaggtta tttccctgtg ctatttttac
     3301 atccagagta ccactaccta ttttatgcag attatctccg gcaacgccct taacattcca
     3361 ttcaacggtg gtattattat caatatctat cccggcacct ttataagact tatcttttcc
     3421 gtacacttta tatgtctggt tcttatcgaa tataagcccg ccaatgccaa gatcggtatc
     3481 ctgcttgagc gttaattccc ctcctcctga gacagacagg tccttatctt ttataatcga
     3541 tgataactct tgttttttac catccagctg catagtgttt gtgaaattct tttttaaatt
     3601 gtttaccaga atattattaa ataacgagta gtttgtccag gctatattgg tactactgag
     3661 aaaaccaact gtactggtaa caccatggaa aacccatttt tgttgttctt tatcatatag
     3721 ataagaacca gaaccactat cgccaaaaat tggtgagttt ttaaaagggg ttctgttggt
     3781 ataaatgtaa ctcttgtttt tccagtccag attaaaaaga cttgcgctta acaagtcggg
     3841 attatagttt tgtccaaaca gatattgttt gccgtttaat atcgttgagg ttgttccggc
     3901 acctgctctg aagccaatta tttgttcctt accgttgtag tttacgccat atcttttcat
     3961 cgcatcttcg ggagaaagtg agaagtcggc actctccgta acccctgttg tttccacgac
     4021 gaatttattc agacgatgaa ccgaaaagtc gccagatgat actctgttac ttgctttata
     4081 tgctgtctga tcccatgact gtgttgttat tgcatgatgc tgtgtaccgt tatgagtcgc
     4141 agtaacactg tacgcacctc caattgatgt tgttgcacct ttgttggaaa cagcagaaaa
     4201 atcaggtaca ggtagttctg gaaaatgaaa tatctttcca tctttcatca ttaattgaac
     4261 atttgttgca cccggtctga actctcctct gttttgagca aggtcaagat aatcgcgggt
     4321 ccatatatta tttatatcca gcattgctgc aaatgctgtt tcgccataac ttccatatat
     4381 tgaggataaa ataataccga ggataacttt ctttcttgat gtaccgacac taactttccg
     4441 ggtcagttca gaaacagcaa ctaatccacc tgtaatatga ctatatttca gtgaataaat
     4501 tttattcata cgtgaactcc gatatttctg agttgtggtt atgggatagc atttaattac
     4561 aacggggaac tatcagtcat ggtacgtaac tgataagggt ataatgataa tcactgtcag
     4621 ttatatttgt atgacagatg taacaaaatg aaaaaaaaga taagcacaag tgattgattg
     4681 gtcagatatt gatcacttgg tgtttgtatt ttgaactttg cgatcaaaat ttgatctttg
     4741 ttgtttccgg tcaatccttt acaggttttc tgtattatcc gggggagtta agtctgtcga
     4801 tgtccgacag acttttgatg atggaaacaa catgcagcga taatgtgcat gctgttttgc
     4861 aggtggaact cagaaggtga actggaactg tgcgaccgca ccgtaagtgt tgtccgtgcc
     4921 gatcaggcct gcaacatcaa ggtgaaccac atcaaacggt gagatcccca ccccggccgt
     4981 gaatgcactg ccgtcgttac cggccagatt ctgacggtaa ccggcgcgca gctgtgccca
     5041 tttccaggca ttaaattctg cgccaatggc ggcaaactga cggttgctgt cggaggtgaa
     5101 accgctggcc ggggtcagat ccacatcaaa tgcggtggtg aacatcgcgt tgtgccagga
     5161 gacaccggcc gtcacctgcg ggcggatcct gaaggtttcc gtgataccgt tcacttcttt
     5221 tgtctcaata ctacggggga tcaggttctg tgcgaccagt cccagcgtcc agttgtcatc
     5281 caggtccgta ctggcaccga tgtcggcgtt gatgccattt ttcgtgttgt ggtagcggtc
     5341 gcctttgaag gcgctgctgt cataatttct gaccagcacg ttgtagttga acaggtcgac
     5401 gcgctggaat ttggggggga agcccagaga ccatttctgc ccggccgttt ccagttccgt
     5461 agccagagca atacccacat cagtgatcag tgccgcgcgg gcaaaggcct gtgatgtcag
     5521 gcggcttttg tccacctcct gcccggtgct gtttgccacc tcttccaggt aagtgaggtc
     5581 agcatcagag actttaccgt cgacactgac ggtgccgtgt gatttaacca tcagtgcggc
     5641 agagaggcga tcaccgggaa gggccgccac agcagaaact cccagctgtg cggcggcatg
     5701 tgtgttacgg aagtcccgga ggtgttcttt caggcgtgcg gcagcctcag gaacaccgtg
     5761 gttactttcg atggcgcggt cgaaggcctt ccactcatca ctgatccggt cggcgttgtc
     5821 ggtgatatta tccggattgg agagctgcgc gccgacggac ggaagcagga tgctgggctc
     5881 atgcgcgacg caaaatccac gatgtatata tcagtactaa aagcgcgacg gcggaagaag
     5941 ctctgaaact aatcggtgaa ctgtacgcca tagagcacga tatacgcggg ttaccggtgt
     6001 ctgaacgcct ggcggtcagg caaatgcaga gtagaccgct cctgacttcc ctgtataagc
     6061 tgatgcagga gaaagaacac acgttatcga agaaatgtcg tctgggagat gcgttccggt
     6121 atatcaggaa gcactgggct gcgttgtgca acttctgtga tgatggcctg gctgaggtag
     6181 ataataatgc agcagaaaga gcgcttcgtg cagtctgtct cgggaagaaa actttttcga
     6241 ggatcactca ttccgtgtct gactgcttca gggtttaatt ttgcttctgc tgactgaatg
     6301 atgattgggc agcaagtgca atatcccaca taaatccagt taactcacga gccaccgccg
     6361 taatggctac ttgtgatttt ttcccttttg ctgtcagagt tcggtaacga gaacacaatc
     6421 gttgttgagc tttccatgca atatctttgg aatattgtgt tacagagtcc ggtcggtaat
     6481 atataagcca tgctccaact tttgcaggtg tacgataaga ccaagcggct tcgtaaagca
     6541 gacgtctcag ttcgctgttt ccaacttttg ttattcctct gggacgaata ctgtttccac
     6601 tggaatattc acctggcacc a
//
LOCUS       DADMKF010000191         6537 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.195, whole genome shotgun sequence.
ACCESSION   DADMKF010000191 DADMKF010000000
VERSION     DADMKF010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6537
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.195"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            142..1317
                     /gene="mdtL"
                     /locus_tag="JNC24_003666"
     CDS             142..1317
                     /gene="mdtL"
                     /locus_tag="JNC24_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HAY9160321.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WAMAMMGIAVLMLSLFILKETRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTVSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            1292..2251
                     /gene="yidZ"
                     /locus_tag="JNC24_003667"
     CDS             1292..2251
                     /gene="yidZ"
                     /locus_tag="JNC24_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HAY9160322.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTPLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQSDTWAL
                     DNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQYYNQLHQLPLVALPL
                     PFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYASMA"
     gene            2408..3157
                     /locus_tag="JNC24_003668"
     CDS             2408..3157
                     /locus_tag="JNC24_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9160323.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPAIEKLSVWELDGKHSWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKSFSPN"
     gene            3179..3729
                     /gene="yieF"
                     /locus_tag="JNC24_003669"
                     /pseudo
     CDS             3179..3729
                     /gene="yieF"
                     /locus_tag="JNC24_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent chromate oxidoreductase YieF"
     gene            complement(3783..5120)
                     /locus_tag="JNC24_003670"
     CDS             complement(3783..5120)
                     /locus_tag="JNC24_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAY9160324.1"
                     /translation="MSHQHTTQTSGQGMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQIGMGAIFWGAVGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANPETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            5286..5951
                     /gene="yieH"
                     /locus_tag="JNC24_003671"
     CDS             5286..5951
                     /gene="yieH"
                     /locus_tag="JNC24_003671"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HAY9160325.1"
                     /translation="MSRIEAVFFDCDGTLVDSEVICSRAYVTMFQEFGITLDPEEVFK
                     RFKGVKLYEIIDIVSLEHGVTLAKTESEHVYRAEVARLFDSELEAIEGAEALLSAITA
                     PMCVVSNGPNNKMQHSMGKLNMLHYFPDKLFSGYDIQRWKPDPALMFHAAKAMNVNVE
                     NCILVDDSVAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTHLSQLPELWKARGWD
                     ITA"
     gene            6018..6491
                     /locus_tag="JNC24_003672"
     CDS             6018..6491
                     /locus_tag="JNC24_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CbrB"
                     /protein_id="HAY9160326.1"
                     /translation="MSVSRRVIHHGLYFAVLGPLIGVLFLVLYIFFAKEPLVLLVIIQ
                     VLPLFLLLSITTGAIPALLTGVMVACLPEKIGSQKNYRCLAGGIGGVVITEIYCAVIV
                     HIKGMASSELFENILSGDSLVVRIIPALLAGVVMSRIITRLPGLDISCPETDSLS"
BASE COUNT         1607 a         1650 c         1652 g         1628 t
ORIGIN      
        1 atttggctaa atccttcaag aagccagcca ttcgctggct tcttgcctct caggaaatca
       61 cttatgtcca aatggcaact cgcctgatcc tccttcacca cgtatgcttt gcgtcacctt
      121 actatcagga cgctttagcc catgtcccgc tttttgattt gtagttttgc cctggtttta
      181 ctttatcccg ccgggattga tatgtacctc gttggtttac cgcgcatcgc cgccgatctc
      241 aatgccagcg aagcgcagtt gcatattgcg ttctccgtat atctggcggg gatggcagct
      301 gcgatgttat ttgccggtaa agtggccgat cgttcaggga gaaagccggt cgccataccc
      361 ggcgcggcgc tatttattat tgcctcggtg ttctgttcac tggctgaaac cagcacgtta
      421 tttcttgcag gccgatttct acaggggttg ggcgcaggct gttgttacgt agtggcgttc
      481 gccattttgc gcgacacgct ggatgatcga cgtcgggcta aagtgctgtc attactcaac
      541 ggtattacct gcatcattcc ggtgttagcg ccagtgctcg gacatctgat tatgcttaaa
      601 ttcccgtggc agagtctgtt ctgggcgatg gcaatgatgg gcatcgcggt actgatgttg
      661 tctttgttta ttttaaaaga aacgcgccca gcgtcccccg ctgcttcgga caaaccacga
      721 gaaaatagcg agtcgctgct taatcggttt ttcctcagcc gtgttgttat caccaccctc
      781 agcgtttcgg tgatcctcac tttcgtcaat acatcgccgg tattgctgat ggaaatcatg
      841 ggttttgagc gcggagaata cgccaccatt atggcgttga ccgctggcgt cagcatgacc
      901 gtttcattct ccacgccatt tgcgctggga atttttaagc cacgtacgtt gatgatcacc
      961 tcgcaggtgt tattccttgc ggcggggatc acccttgccg tttcaccttc ccatgcggtt
     1021 tctctgtttg gtatcacgct gatttgcgcc ggtttctcgg taggttttgg cgtagcgatg
     1081 agtcaggcgt taggaccatt ttcattacgc gcgggcgtag ccagctcgac cttaggtatt
     1141 gcgcaggttt gcggttcgtc actgtggatt tggctggcag cggtggttgg tattggcgca
     1201 tggaatatgc tgatcgggat tctgattgcc tgtagcatag tgagcctgtt gctgattatg
     1261 ttcgtcgcgc ctggacgccc cgttgccgct catgaagaaa tccatcacca cgcttgatct
     1321 caatctgctg ctctgtctgc aactgctgat gcaggagcgc agcgtgacca aagcggcgaa
     1381 gcggatgaac gtgacccctt cagcggtgag taagtcgctg gcaaagttaa gagcgtggtt
     1441 tgacgacccg ctctttgtga actcaccgct gggcctgtcg cccacaccgc taatggtcag
     1501 catggagcaa aatctggcgg aatggatgca aatgagcaat ctgctgctgg ataaaccgca
     1561 ccaccagaca ccgcgcggcc tgaagtttga gttggcggcg gaatcgccgc tgatgatgat
     1621 catgcttaat gcgctgtcga aacggatcta ccaacgttac ccgcaggcga ccatcaaact
     1681 acgtaactgg gattacgatt ccttagatgc cattactcgt ggtgaagtgg atattggttt
     1741 ttccggtcgc gaaagccatc ctcgctcgcg ggagctgtta agctcgctac cgttagccat
     1801 tgattatgaa gtgctgttta gtgatgtacc ctgcgtctgg ttacgcaaag atcatccggc
     1861 actgcatgaa acgtggaatc tggacacctt tttacgttat ccgcatatca gcatttgctg
     1921 ggaacagagc gacacctggg cgctggacaa tgtgttacag gagctggggc gcgaacgcac
     1981 gattgctatg agtctgccgg aattcgagca gtcactgttt atggcagcgc aacccgacaa
     2041 tctgctactg gcgaccgcgc cgcgctactg tcagtactac aatcaactcc atcaactgcc
     2101 gttggttgct cttcctctcc cgtttgacga aagccagcaa aaaaagctgg aagttccttt
     2161 taccctgctg tggcataaac ggaacagcca taatccgaag atcgtctggt tacgggaaac
     2221 cattaaaaac ctttacgcgt cgatggcata accgaatcgt atgaaacgcg acccaatttc
     2281 acaataaaat gtaaaaaagt tgtaacaagc ttgtctgaat cgaactttta gccgctttag
     2341 tctgtccatc attccagtaa atgattactc ttgtattcat aatggaccat taagcatgga
     2401 gcgaaaaatg gcgactcact ttgcccgagg gattttaacg gaaggacatc tgatttctgt
     2461 tcgtctcccc tcccagtgtc atcaagaagc ccgaaacatt ccccctcatc gtcaaagccg
     2521 ttttctggcg tccagaggtt tactcgcaga actgatgttc atgctgtatg gcattggcga
     2581 attgccggaa atcgtcaccc tgccgaaagg taaaccggtt ttcagtgata aaaatttgcc
     2641 ttcgttttct atttcctatg ccgggaatat ggttggcgtg gcgttaacaa ccgaaggtga
     2701 atgtggcctc gatatggaac tacagcgtgc gacgcgcggg tttcatagcc cacacgcgcc
     2761 cgataaccac accttttcca gcaatgaatc gctatggatc agtaaacaaa acgatcctaa
     2821 cgaagcgcgg gcgcagctca tcacgctgcg ccgaagcgtg ctaaaactaa ccggtgatgt
     2881 tttgaatgac gatccgcgcg atctgcagct gctgcccatt gccggacgcc tgaaatgtgc
     2941 tcatgtaaat catgtagaag cgttatgcga cgcggaggac gtgctggtgt ggtccgtggc
     3001 ggtcacgccc gcgattgaaa agctcagtgt ctgggagtta gatggcaaac acagctggaa
     3061 aagcctgccg gatattcaca gccgcgccaa caatcctacc agccggatga tgcgttttgc
     3121 ccagctctct accgtgaagt ctttttcgcc aaattgatag acaaccacag gagtcattat
     3181 gtctgaaaaa ttgcaggtgg ttacgttact ggggagcctg cgcaaaggct catttaatgg
     3241 catggttgca cgtaccctgc cgaaaattgc tccggcgagc atggaagtca atgcgttacc
     3301 ttccattgcc gacattcccc tgtatgacgc tgacgtacag caggaagaag gttttccagc
     3361 aacggttgaa gctctggcgg aacagatccg tcaggctgac ggtgtggtga tcgtcacgcc
     3421 ggaatataac tactctgtac cgggtgggct gaaaaatgcc atcgactggc tttcccgctg
     3481 gccggtaaac cggtgttgat tcagaccagc tcaatgggcg tgattggcgg cgcgcgctgt
     3541 cagtatcacc tgcgccagat tctggttttc ctcgatgcaa tggtgatgaa caagccggaa
     3601 tttatgggcg gcgtgattca gaacaaagtt gatcggcaaa ccggagaagt gattgatcag
     3661 ggtacgctgg accacctgac cgggcaattg accgcatttg gtgagtttat tcagcgagtt
     3721 aagatctaaa taaaaaaccc gccagtaatc atgcatggcg ggtttttaat gcgctatcga
     3781 ttttaatgag cgtcgataaa tacaatcttc aggataaaca gcagcgcaac gatgattacg
     3841 cacgggctaa ggtcacgcag acgcccggta ccaatcttca tcacgcagta ggagataaag
     3901 cccagcgcaa taccttcggt aatcgaaaag ctgaacggca tcatcacggc ggtaataaac
     3961 gccggaacag attcagtaag atcctgccag ttcacgcgag caagacttga ggtcatcaac
     4021 acgccaacgt agatcaacgc gccagctgca gcgtagcctg gcaccattcc cgccagcggc
     4081 gacagaaaga taaccagcag gaacaacaga ccaacaacca ctgccgtcag accggtacga
     4141 ccgccaaccg atacgccgga agaggactca atataagccg taacggaaga agtaccgata
     4201 aacgaaccgg tcacggaaga gatactgtcg acatacagcg cctgcttcat gcgcgggaat
     4261 ttccccttct catccgccag acctgcttta tcggtcacgc caatcagcgt accggaggag
     4321 tcaaacaagt tgaccaacat gaaagagaaa atcacccctg ccagcccgag gttaaacgac
     4381 ccggctaaat ctacatgacc cacaactgtc attacgctcg gcggcgcaga aacgatgcca
     4441 ttgtagtgca catcacccag catccagccc agcagcgtcg tcaccacgat agaaaccagc
     4501 accgctgcgt gaatgttgcg cgaggccaga atggcgatga tgaagaaacc gaggatcccc
     4561 agcagcacgc tgtgagaagt cagattaccg atgctcacca gcgtttccgg gttagcgaca
     4621 atcacacctg cgtttttcag ccccatcatg ccaatgaaca gaccgatacc gctggtaata
     4681 cccacacgca gacttaccgg aatgttagca atcatccagt aacgaacgcg gaaaatggtc
     4741 aaaagaagca gtccgacagc gccccagaag attgcgccca taccgatctg ccacggcagc
     4801 cccattgcct ggacaaccac aaaagcgaaa aaggcgttaa gccccatcgc aggagccagc
     4861 gccaccggaa ggtttgcaaa caatcccatc ataatgctac cgaaagcagc aatcagacag
     4921 gttgttacga agacggcact ggtgtccatg ccagcaacgc ccagaatttg tgggttaaca
     4981 aatacgatgt agaccatcgt caggaaggtg gtgaacccgg caatcacttc agttcgtgcc
     5041 gtagtaccat gttcgcgcag tttaaaaact cgttccagca tcccctgacc agaagtctgg
     5101 gtggtatgtt gatgactcat tatctatttc cgaacaagga gggaaaatcc gtcgctatcg
     5161 tataccaaaa tgcgacaata ggcgcgtttg tgagagactt tttttattgg atttacttat
     5221 acggcaacga ttgcgttgcg caaatcggct ctacgaaaac gttaaactga ttaaaaagga
     5281 aaggcatgtc ccggatagaa gcggtatttt tcgactgcga cggtacgctg gtcgacagtg
     5341 aagtcatttg ctcccgcgca tatgtaacga tgtttcagga atttggtatt acgctcgatc
     5401 ctgaagaggt attcaaacgt ttcaaaggtg taaaactgta cgaaattatc gatattgttt
     5461 cccttgaaca tggtgttacg ttagcgaaaa cagaatctga acacgtttac cgtgcagaag
     5521 tcgctcggct gttcgattca gaactggaag ccatcgaagg ggctgaagcg ctcctgtcag
     5581 cgatcactgc gccaatgtgt gtggtatcta acggcccaaa taacaaaatg cagcattcta
     5641 tgggcaagct gaatatgttg cactacttcc cggataaact gttcagcggc tacgatattc
     5701 agcgctggaa gccagacccg gcgttaatgt tccatgcggc aaaagcgatg aatgtaaatg
     5761 tagaaaactg cattctggtt gatgactcag ttgccggtgc gcaatctggt atcgacgcag
     5821 gtatggaagt gttctacttc tgcgccgacc cgcacaataa gccgatcgtt cacccgaaag
     5881 tcaccacctt tacccatctt tcgcagttac cggaactgtg gaaagcgcgt ggttgggata
     5941 ttacggcata gttcttcaca ctcccttcac ttaccccgct taaattggcg ctcaaaggta
     6001 agtaaaggga gtttgatatg tctgtttcac gtcgggtaat acatcacgga ctttattttg
     6061 cggttttagg accgttaatt ggtgttctgt ttcttgtcct ctacatattc ttcgcaaaag
     6121 aaccgctggt tcttttggta ataatacagg tacttccatt gtttctctta ttgtcgatta
     6181 ctacgggagc tattcctgca ttgttaaccg gcgtgatggt cgcctgtctg ccggaaaaga
     6241 tcgggtcaca gaaaaattat cgttgtctgg ctggtggcat aggtggcgtc gttatcaccg
     6301 agatctattg tgcagttatt gtacatatta agggcatggc ttcctcggag ttgtttgaaa
     6361 acattctttc tggtgacagt ctcgttgtcc gcatcattcc tgcattgctg gcaggtgtgg
     6421 tgatgagcag aatcattacc cgtctgcccg gattggatat ttcatgtcct gaaacagact
     6481 ctttaagtta agcgggatac tttatctttg ggctactcaa aagcagacag gatgtta
//
LOCUS       DADMKF010000192         6515 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.196, whole genome shotgun sequence.
ACCESSION   DADMKF010000192 DADMKF010000000
VERSION     DADMKF010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6515
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.196"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            38..250
                     /locus_tag="JNC24_003673"
     CDS             38..250
                     /locus_tag="JNC24_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9160327.1"
                     /translation="MSNTQKIINTEKYNEWVKKFSEQIFKITGDENVAKNELEPWTPE
                     GNAPNYCWWEVDPVDAANEAMSYHND"
     gene            283..501
                     /locus_tag="JNC24_003674"
     CDS             283..501
                     /locus_tag="JNC24_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 domain-containing protein"
                     /protein_id="HAY9160328.1"
                     /translation="MTTLFRKEYPQKSRATEFLFLILFIVLMIPISPLIFVWAIGKII
                     EPVTELYTDVVWASFNTLHNKINPYKEN"
     gene            572..889
                     /locus_tag="JNC24_003675"
                     /pseudo
     CDS             572..889
                     /locus_tag="JNC24_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309784.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(922..1560)
                     /gene="ompW"
                     /locus_tag="JNC24_003676"
     CDS             complement(922..1560)
                     /gene="ompW"
                     /locus_tag="JNC24_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HAY9160329.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDAGSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDYWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTAKYKSGVTTVKDSVRLDPWVFMFSAGYRF"
     gene            1800..1889
                     /locus_tag="JNC24_003677"
                     /pseudo
     CDS             1800..1889
                     /locus_tag="JNC24_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            1917..2660
                     /locus_tag="JNC24_003678"
     CDS             1917..2660
                     /locus_tag="JNC24_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0259 family protein"
                     /protein_id="HAY9160330.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSGLIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            2690..3229
                     /locus_tag="JNC24_003679"
     CDS             2690..3229
                     /locus_tag="JNC24_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HAY9160331.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLGKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            3334..3732
                     /gene="yciA"
                     /locus_tag="JNC24_003680"
     CDS             3334..3732
                     /gene="yciA"
                     /locus_tag="JNC24_003680"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HAY9160332.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(3772..4491)
                     /gene="tonB"
                     /locus_tag="JNC24_003681"
     CDS             complement(3772..4491)
                     /gene="tonB"
                     /locus_tag="JNC24_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HAY9160333.1"
                     /translation="MTLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAPAQP
                     ISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKP
                     VKKVQEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALSRNQ
                     PQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRYEPG
                     KPGSGIVVNILFKINGTTEIQ"
     gene            4582..6102
                     /locus_tag="JNC24_003682"
     CDS             4582..6102
                     /locus_tag="JNC24_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAY9160334.1"
                     /translation="MRTVITYLRFSSAIQGAEGADSTRRQNDLFKQWLKKNGDAQIVA
                     SFSDEGLSGYKGKHLTGQFGDMLARIEAGEFPEGTILLVESIDRIGRLEHLETEALMN
                     RILGNGIEIHTLQDGLIYTKDALADDLGISIIQRVKAYIAHQKSKQKSFRVSQKWGQR
                     AKLALAGEQRLTKMVPGWIDPETFKLNEHAETVRLIFKLLLDGESLHNIARHLQSNGI
                     KSFSRRKDANGFSVHSVRTILRSETTIGTLPASQRNDRPAIPNYYEGVVDIPTFNKAQ
                     EILDKNRKGRTPASDNPLTINIFKGLFRCQCGASVHPTGTKNKYAGVYRCNNHLDGRC
                     DVPPLKRKPFDRWMIDNFLGMIDVGNDGESERKIAALQHEVEIVTARIKKATALLLEM
                     DDIDELKIQLKELNQKRTELQTTIDNMRRKASLTDKELPQLKDIDLMTKAGRVECQLI
                     LSKHLKGLTLGKDSVTVTLQNDTEITIPTNPLPLNDGSPIFEIADKELLDIDAYQL"
     gene            complement(6135..>6515)
                     /locus_tag="JNC24_003683"
     CDS             complement(6135..>6515)
                     /locus_tag="JNC24_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001460821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9160335.1"
                     /translation="IANGLRIQMTFQGVEFRSIVGQTDVSAATGKTATTNDGGATSKK
                     NTGNTTTSLGSPMLTCKELFGYSASELSDEALKARVTCSKSVSVKNGESTFSDAANEQ
                     ASKVLKPGNALRKYSVNPNKKGTY"
BASE COUNT         1714 a         1489 c         1550 g         1762 t
ORIGIN      
        1 tgtaaataaa atttaatcct taactggagg tatatctatg tcaaatacac agaaaattat
       61 taacactgaa aaatataacg agtgggtgaa aaaattctct gagcagattt ttaaaattac
      121 tggcgacgag aatgtggcaa aaaatgaatt agaaccgtgg acacctgaag gaaacgcacc
      181 aaattattgc tggtgggagg ttgatccggt tgatgctgca aatgaagcca tgagttacca
      241 caacgattaa tgtcgggagg ccgcccgaaa gggcggtaag aaatgactac attattcaga
      301 aaagaatatc cgcaaaaaag tagggcgaca gaatttttgt ttctcattct gtttatcgta
      361 ttgatgatac cgatatcccc tctaattttt gtctgggcaa tcgggaaaat aattgagcca
      421 gttactgaat tgtataccga cgttgtatgg gcgtcgttca acacactgca caataaaatt
      481 aatccgtata aggaaaactg atatggcaac tttgacaaaa aaagaacggg catggttgaa
      541 cgaattacag gaagttcttg atcgctgtcc tgtggtgtca tcctgccgcg attgcgttct
      601 tagtccgtgg ctattgcacc accatcacgc gaaagggaca gctaagcgcg gcgggatggt
      661 taagccagca acattaaccg ttgcatttaa aaaagcccgg gattctgtgg attacaactg
      721 gcgtgctaat ggcaccccac cctctttcca tgagcagaga tctttatcag agcgattgtt
      781 cagagagcag ggggttgata ccaaaatttt gctaggccat tcgaatcaaa aaatgaccga
      841 tatttacaac gatgtacgcg gtaaggagtg gaaaaaactg gtcatttgat aaccagtttt
      901 gcagaggttt tgcagagaaa attaaaagcg gtacccggca gagaacataa acacccacgg
      961 atccagtcgt accgagtctt ttacggtagt aacaccagat ttgtacttag ccgtggtatc
     1021 gatatccatg taccacactg acatgttaac caaccagtca cggttaatca gataatcaac
     1081 ccccacctgc ccggcagctc cccagtaatc tttcagactg agatcggaaa gccccgcctc
     1141 tttgccatga tcgttaaatc cattatcaaa gaaggtggtg tagttaatac ctgccccaac
     1201 ataaggacgg aatttgctgc cggcatcacc aaaataccac tgcgccatca gtgttggcgg
     1261 cagatgatga acggttgcaa tatcgccggt cgcccgggtg ccgattttat ggcggaacgg
     1321 cgtcgctgcc agtaattcca caccaatgtt gtcggtcgcc atataagtaa acgtcaggcc
     1381 cagttgcgtg ttattggtca cgctgaatcc acccagactt cctaacgtac caccagcacc
     1441 ttctgttgga cgtacggttg cagaacctgc acgcataaaa aattcgcctg cttcatgcgc
     1501 aaaggcactg ccagagagaa gagttgttac tgccaaagcc gccactgtta actttttcat
     1561 atccgctccg tcgttatggt tataaaaacg tgacgaatat acctacattg gagtaacaaa
     1621 tgatcctaca tagatcacat tacatatgat aattcaccct ttattgatct ggattaattt
     1681 agaagttgca ttgagaatcc tggttaacgt gatttaaatc aatttttaga tattttcgct
     1741 acgcaaatat taactcgttc gccacttgcc gcggtttgcc cacgcctgaa ggccagcaat
     1801 tacctccgct ttacaaagat atgcttttcc atacaagacc tgatgctgcc acccgaggct
     1861 taaccagggt acaattgccc gctaattaac acctgcataa aactcaagga gagtgcatgt
     1921 ctatcacggc gcagtccgta taccgtgaca ccggaaattt cttccgtaat caatttatga
     1981 ccattctgtt ggtatcgttg ctatgtgcgt ttatcacagt ggtgttaggg catgttttct
     2041 cacccagtga tgcacagctt gcgcagctca atgacggcgt gcccgttagc ggcagtagtg
     2101 ggttgttcga cctggttcag aatatgtcac cggaacaaca acaaattttg ctgcaggctt
     2161 cagcggcgtc cactttttca ggattaatcg gtaacgccat tctcgccgga ggcgtaatat
     2221 taattatcca gctggtgtct gcgggtcaga gagtcagtgc gctgcgagct attggtgcca
     2281 gtgcgccgat attgcccaag ttatttattc tgatttttct gactaccctt ttagtacaga
     2341 ttggcatcat gctggtggtc gttccgggaa ttatcatggc cattttactg gccctggcac
     2401 cggtgatgct ggtacaggac aaaatgggca tttttgcctc gatgcgtagc agtatgcggc
     2461 tgacttgggc gaatatgcgt ctggtggcac ccgcagtact gagctggttg ctggcaaaaa
     2521 cactgttgct gctttttgcc tcttcttttg ccgcattaac cccggaaatt ggtgccgtac
     2581 tggcgaacac cttgagcaac ctgatttcag ccatattgct catctatctg ttccgcctgt
     2641 atatgttgat tcgccaataa ccttaagcat ttgattacgg aatcgtaaaa tgaagcagtt
     2701 tcttgatttt ttaccgctgg ttgtcttttt cgcgttttac aagatttatg acatctatgc
     2761 ggctactgcg gcgctgatcg tcgccacggc gattgtgctt atctatagct gggttcgctt
     2821 tcgtaaggtt gagaagatgg ccctgatcac ttttgttctg gtggtcgtct tcggtggctt
     2881 gacgctgttc ttccacaatg atgagtttat taaatggaag gttacagtca tttatgctct
     2941 gtttgcggga gccctgttag tcagccaatg ggtgatgaaa aagccgctaa ttcagcggat
     3001 gctgggtaaa gaactcacgc tgccgcaacc ggtatggtcg aagctaaatc tggcctgggc
     3061 tgttttcttt atcctttgcg gtctggcaaa catctacatc gcattctggc tgccgcaaaa
     3121 tatttgggtc aactttaaag tctttggcct gaccgccctt accttaatct tcacattgtt
     3181 aagcggtatc tatatctacc gccacatgcc gcaggaagat aaatcctaac cagactgcca
     3241 gtccttcggg gctggcgctg tatctcctct gttctcctca ggaaaatcat agtagcatcg
     3301 cgcctgtgat tttcctttta agtcggtttt accatgtcta caacacataa cgtccctcag
     3361 ggcgatcttg ttttacgtac tttagccatg cccgccgata ccaatgccaa tggtgacatc
     3421 tttggtggtt ggttaatgtc acaaatggat attggcggcg ctattctggc aaaagaaatt
     3481 gcccacggtc gcgtagtgac tgtgcgggtt gaaggaatga ctttcttacg gccggttgcg
     3541 gtcggcgatg tggtgtgctg ctatgcacgc tgtgtccaga aagggacgac atcggtcagc
     3601 attaatattg aagtgtgggt gaaaaaagta gcgtctgaac caatcgggca acgctataaa
     3661 gcgacagaag cattatttaa gtatgtcgcg gttgatcctg aaggaaaacc tcgcgcctta
     3721 cctgttgagt aatagtcaaa agcctccggt cggaggcttt tgactttcta cttactgaat
     3781 ttcggtggtg ccgttaattt taaacaggat attcaccaca atcccactgc ctggcttacc
     3841 cggctcataa cgccatctgc gcatcgcatt tttcacctca cgctcaaaca tgttcgcagg
     3901 cttggctgag aggatttgta cgttatccac gcgaccatcc ggcgtgacgt caaatttaac
     3961 tttaacctgc ccttcaatgc gcaatgcctg tgctcgtgcc ggatactgcg gctgattacg
     4021 gcttaatgcg cgtggtcctg aagccacact ggtaaccggc ttgctggttg cagccgttgc
     4081 cgtacttgat gtggggcgcg cgggtgccgt attttcaaac ggtgatgccg gacgcgactc
     4141 tacgggtttg acatcgcgtt ttggctgctc ctgtaccttt ttcaccggct tcggttttgg
     4201 cttaggtttc ggcttcggct tttcaatgac caccggtgct tcttttggcg gttcggggat
     4261 cggctcaggt tccggttctg gctctaccac cggctccggt ggcggctgaa cggcttgtgg
     4321 cggttcgaga tcagcaggcg caaccatcgt gacagaaatt ggctgcgcag gcgcaggtag
     4381 ttcaataacc tgatgtaccg aggtatagag cagacccgcc acaacagcac catgaatgca
     4441 gaccgaaagt aacgtcggcc aggggaagcg gcgaggtaaa tcaagggtca ttgtattgta
     4501 gctattcggc attgtagcat tagccataac gtttataagt tcatctttaa atgataatca
     4561 attgcatggg agtttatcac cgtgagaacc gtaataacat acttgagatt ctcatcagcc
     4621 atacaaggcg ctgaaggtgc agattcaacc agacgacaaa acgacctgtt caagcagtgg
     4681 ctgaagaaga atggcgatgc tcaaatagta gcgtctttca gtgatgaagg gttgagtggt
     4741 tacaaaggta aacaccttac tggtcagttt ggtgacatgt tagcccgtat tgaggctggg
     4801 gagtttccag aaggtacaat tctgctagtt gagagtatcg accgtatagg ccgattagag
     4861 catcttgaaa cagaagcctt gatgaaccgc atcttaggca acgggataga gattcacaca
     4921 ttgcaagatg gccttatcta cacaaaggat gctttagccg atgaccttgg cataagcatc
     4981 atccagcgtg tcaaagccta catagcgcat cagaaatcta agcagaagtc tttccgtgtt
     5041 tctcagaaat gggggcaacg tgcaaaactt gcccttgctg gtgaacaacg tcttacaaag
     5101 atggttcctg gatggattga ccctgagact tttaaactca atgaacacgc tgagactgtg
     5161 agattgatat tcaaactgct gctagatggt gaaagtctgc ataacattgc acgtcacctt
     5221 caaagcaacg gtataaagtc gtttagtcgc cgtaaagatg ctaatgggtt ctctgttcac
     5281 tctgtacgca ccattctaag gtcagagaca acaataggca cgttaccagc atcacaacgt
     5341 aatgaccgcc ccgctatacc gaactactac gaaggtgttg tagatatacc aacgttcaat
     5401 aaagctcaag agattctcga caagaatcgt aaaggccgta cacctgcaag tgacaaccca
     5461 ctaacgatta acatcttcaa aggtctgttt aggtgtcagt gtggggctag tgtccatcct
     5521 accggaacaa agaataagta tgctggggtc tacaggtgca ataaccactt agacggtcgc
     5581 tgtgatgttc caccgttgaa gcgtaaaccg tttgaccgat ggatgattga taattttctg
     5641 gggatgattg acgtggggaa tgatggagaa tcagagagaa agattgcagc gttacagcat
     5701 gaggttgaaa ttgtcacagc cagaatcaag aaagctaccg ccctacttct tgagatggat
     5761 gatattgatg aactaaaaat tcagcttaag gaactgaacc agaagcgcac agaactacag
     5821 accacgattg ataacatgag gcgtaaggct tcactcactg ataaggaact accccagctt
     5881 aaagatattg accttatgac taaagcgggt cgtgtcgagt gtcagttgat tttgtccaag
     5941 catctaaaag ggcttacatt gggtaaggat tcggtaactg taacgctaca gaacgacact
     6001 gaaatcacca tacctacaaa cccgttaccg ctaaatgatg ggtcaccaat ctttgaaatt
     6061 gctgataaag agctactgga tattgacgct tatcaactgt aattttcagg ggctatgaag
     6121 ccccttttta tgtctcaata agtgcccttc ttgttagggt tcacagagta ctttcgcaga
     6181 gcattacccg gcttaagcac ctttgaagct tgctcattag cggcatctga gaaggtgctc
     6241 tcaccatttt taacgctaac gctcttagag cacgttacac gggcttttag cgcttcatct
     6301 gataactcac ttgccgaata gccaaaaagc tctttacagg tcagcatagg gcttccaagg
     6361 ctcgttgtag tgtttccggt gttcttttta ctggtagcac cgccatcatt cgtggtagcg
     6421 gtctttccag tagcagccga aacatcagtc tgacctacga ttgaacgaaa ctctacacct
     6481 tggaaggtca tctgaatacg aagaccgtta gcaat
//
LOCUS       DADMKF010000193         6450 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.197, whole genome shotgun sequence.
ACCESSION   DADMKF010000193 DADMKF010000000
VERSION     DADMKF010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6450
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.197"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            <1..331
                     /locus_tag="JNC24_003684"
     CDS             <1..331
                     /locus_tag="JNC24_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HAY9160336.1"
                     /translation="IEAWRTEQEAKPFTFEWNGRIWNAGPNSLGRLYPVVMAAKSDIV
                     RDVMTWGDADNQQVKQSMPELEELAAAMAQAQVERNDEIYRRQRELKEELNSLKDLNS
                     VRNFIVE"
     gene            complement(532..1389)
                     /gene="sitD"
                     /locus_tag="JNC24_003685"
     CDS             complement(532..1389)
                     /gene="sitD"
                     /locus_tag="JNC24_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HAY9160337.1"
                     /translation="MMALLLEPLQFTFMSHALLISLVVSIPCALLSVFLVLKGWALMG
                     DAMSHAVFPGIVLTWILGLPLATGAFVAGVFCAVATGYLKDNSRIKQDTVMGIVFSGM
                     FAAGLILYIAIKPDVHLDHILFGDMLGITIGDIIQTVIIAGLVTLVISAKWRDFLLFS
                     FDYQQAQVSGLHTRWLHYGLLCMVSLTIVATLKAVGIILSISLLIAPGAIAVLLTQRF
                     HIALLLATGISVIVSMTGVWLSFFIDSAPAPTIVVLFAVVFIMTFTVTSINARTKGNA
                     HTQDLLSPN"
     gene            complement(1386..2243)
                     /gene="sitC"
                     /locus_tag="JNC24_003686"
     CDS             complement(1386..2243)
                     /gene="sitC"
                     /locus_tag="JNC24_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HAY9160338.1"
                     /translation="MNVLLEPFSYEYMLNAMWVSAMVGGLCAFLSCYLMLKGWSLIGD
                     ALSHSIVPGVAGAYMLGLPFSLGAFFSGGLAAGSMLFLNQRTRLKEDAIIGLIFSSFF
                     GLGLFMVSLNPTSVNIQTIVLGNILAIDPADILQLTIIGILSIIVLFFKWKDLMVTFF
                     DENHARAIGLHPGRLKILFFTLLSVSTVAALQTVGAFLVICLVVTPGATAWLLTDRFP
                     RLLIIAVTIGSVTSFLGAWVSYFLDGATGGIIVVAQTLLFLLAFVFAPTHGLLANRRR
                     AHKALEDRS"
     gene            complement(2240..3067)
                     /gene="sitB"
                     /locus_tag="JNC24_003687"
     CDS             complement(2240..3067)
                     /gene="sitB"
                     /locus_tag="JNC24_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="HAY9160339.1"
                     /translation="MMQSAGIVVNDVTVTWRNGHTALRDASFTVPSGSIAALVGVNGS
                     GKSTLFKAIMGFVRLTSGKISVLGIPTRQALQKNLVAYVPQSEEVDWSFPVLVEDVVM
                     MGRYGHMGMLRIAKKRDRQIVTDALERVDMVDFRHRQIGELSGGQKKRVFLARAIAQQ
                     GDVILLDEPFTGVDVKTEAKIISLLRELRAEGKTMLVSTHNLGSVTTFCDYTVMVKGT
                     VLASGPTDTTFTAENLELAFSGVLRHVTLNDSEESIITDDERPFVAHRPSAVQREAR"
     gene            complement(3067..3957)
                     /gene="sitA"
                     /locus_tag="JNC24_003688"
     CDS             complement(3067..3957)
                     /gene="sitA"
                     /locus_tag="JNC24_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707262.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HAY9160340.1"
                     /translation="MLLGCLALTCSIAFQASATEKFKVITTFTIIADMAKNVAGDAAE
                     VSSITKPGAEIHEYQPTPGDIKRAQGAQLILANGMNLELWFQRFYQHLNGVPEVIVSS
                     GVTPVGITEGPYEGKPNPHAWMSPDNALIYVDNIRDALIKYDPANAQTYQRNADTYKA
                     KITQTLAPLRKQITELPENQRWMVTSEGAFSYLARDLGLKELYLWPINADQQGTPQQV
                     RKVVDIVKKNHIPAVFSESTISDKPARQVARETGAHYGGVLYVDSLSTENGPVPTYID
                     LLKVTTSTLVQGIKAGKREK"
     gene            complement(4567..5001)
                     /gene="uspF"
                     /locus_tag="JNC24_003689"
     CDS             complement(4567..5001)
                     /gene="uspF"
                     /locus_tag="JNC24_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HAY9160341.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            complement(5142..6275)
                     /gene="ompN"
                     /locus_tag="JNC24_003690"
     CDS             complement(5142..6275)
                     /gene="ompN"
                     /locus_tag="JNC24_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
                     /protein_id="HAY9160342.1"
                     /translation="MKSKVLALLIPALLAAGAAHAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNSAKDGDQSYARLGFKGETQINDQLTGYGQWEYNIQANNTESSKNQSWTRLAFAGL
                     KFADYGSFDYGRNYGVMYDIEGWTDMLPEFGGDSYTNADNFMTGRANGVATYRNTDFF
                     GLVNGLNFAVQYQGNNEGASNGQEGTNNGRDVRHENGDGWGLSTTYDLGMGFSAGAAY
                     TSSDRTNDQVNHTAAGGDKADAWTAGLKYDANNIYLATMYSETRNMTPFGDSDYAVAN
                     KTQNFEVTAQYQFDFGLRPAVSFLMSKGRDLHAADGADNPAGVDDKDLVKYADVGATY
                     YFNKNMSTYVDYKINLLDEDDSFYAANGISTDDIVALGLVYQF"
BASE COUNT         1759 a         1517 c         1556 g         1618 t
ORIGIN      
        1 aattgaagca tggcgcacag aacaggaggc gaagccgttc acgtttgaat ggaacggtcg
       61 tatctggaat gctggcccca actcactggg ccgcctttat ccggtggtaa tggctgcgaa
      121 atccgacatt gtacgggacg tgatgacgtg gggtgatgcc gataatcagc aggtgaaaca
      181 gtcgatgccg gaactggaag aactggcggc agcaatggct caggcacagg ttgagcgcaa
      241 tgacgagatt tatcgccgtc agcgggagct gaaggaagag ctgaatagtc tgaaggattt
      301 gaattcggtt aggaatttta tcgtggaata acagaagctg cggcacgtcg tatgcaggaa
      361 cgtgccgcgg ttggctggta aactttcgat agtgcgagta ttgaatgatt tctaggcgtt
      421 atcgatttta cgtatttttt gcatgataga attcgtacct cctcccaacg accttccatg
      481 gctttccgga cttctgtagg ctggcggacg atgctgtcct cggatgttgt gctaattggg
      541 tgataacaga tcctgtgtat gggcgtttcc ctttgtgcgt gcgttgatgc tggtaacggt
      601 aaacgtcatg atgaacacga ccgcgaataa gacgacaatc gtcggagctg gtgcgctgtc
      661 gataaaaaag gacagccaga caccagtcat tgacactatt accgatatgc cagtcgccag
      721 taggagcgca atatgaaagc gttgtgtcag gagtactgca atggcgccag gcgcaatgag
      781 cagagaaata gagagaatga tacctactgc tttcagcgtc gccacaatgg tcagggaaac
      841 catgcacagc agcccataat gcagccatct cgtatgcaaa ccgcttacct gcgcctgttg
      901 ataatcaaag ctgaacagca aaaaatcccg ccattttgcg ctaataacaa gtgtaaccag
      961 cccggcaata atcaccgtct ggattatatc gccgatggtt atcccgagca tgtcgccgaa
     1021 aagaatatgg tcaagatgta cgtctggttt gattgcaata tacaagatca gacctgcggc
     1081 aaacatgcca gaaaaaacaa tccccataac tgtatcttgc ttgattcggc tgttgtcttt
     1141 caggtatccc gtcgccaccg cgcagaatac tccggcgacg aatgcccctg tagccagagg
     1201 taaccccaga atccaggtaa gcacgatgcc aggaaaaacc gcatgactca tcgcgtcacc
     1261 catcagtgcc caacctttca ggaccagaaa gaccgacagc agcgcacagg gtatggaaac
     1321 taccagtgaa atcagcagtg cgtgactcat aaaggtgaac tgcagtggct ccagtaataa
     1381 tgccatcatg agcgatcctc cagcgcttta tgcgcacgcc gacgatttgc caaaagtcca
     1441 tgggtgggcg caaatacaaa tgctaaaaga aacagcagtg tctgcgccac cacgatgatt
     1501 ccacctgtcg cgccgtccag aaagtaactg acccacgcgc cgaggaaact ggttacactg
     1561 ccaatagtta cagcaattat gagcaggcgg ggaaaacgat cggttaacaa ccatgctgtt
     1621 gcaccgggag tcaccaccag acagatcacc agaaaggcac caaccgtttg cagcgccgca
     1681 acggtcgaaa ccgacagcag ggtgaagaag aggattttta atctgcccgg atgtaatcca
     1741 atagcacggg cgtgattctc gtcgaaaaag gtcaccataa gatctttcca tttgaaaaaa
     1801 agcacgatga tcgagagaat gccaatgata gtgagttgca ggatatccgc cggatcgatt
     1861 gccagaatat tgcccaggac gatggtctga atgttcacgg atgtcgggtt caatgacacc
     1921 ataaacagcc ccaggccaaa aaaggacgag aagatcaggc cgatgatggc atcttctttt
     1981 aagcgggtgc gttggttaag aaacagcatg ctgcccgctg ccagtccgcc ggaaaagaag
     2041 gctcccagag aaaacggaag ccccagcata tatgcccccg caacacccgg gacaatagag
     2101 tgcgacagcg catcgccaat cagcgaccag ccttttagca tcagatagca tgacaggaaa
     2161 gcgcagagac cgccgaccat tgccgagacc cacatcgcat tgagcatata ttcatagctg
     2221 aagggttcca gtagcacgtt catcttgctt ccctctgcac cgctgacggt cgatgtgcca
     2281 caaaagggcg ttcatcgtca gtaataatgc tttcttccga gtcgttaagt gtgacatggc
     2341 gcagtacgcc gctaaaagcc agctccaggt tctcggctgt aaaagtggtg tctgtcggcc
     2401 cgcttgccag gacggtgcct ttgaccatga ccgtataatc gcaaaatgtc gtgactgagc
     2461 cgagattgtg cgttgagaca agcatcgttt tgccttccgc gcgtaactcc ctcaacaggc
     2521 tgatgatttt cgcttcggtt ttcacatcaa cgccggtaaa aggttcatcc agcaggatca
     2581 catcaccctg ctgtgcaatc gctctcgcca gaaagacgcg tttcttttgt ccaccggaaa
     2641 gctcgccgat ttgacgatgg cgaaaatcca ccatatcgac acgttccagc gcatcggtga
     2701 caatctgacg atccctcttt ttggctatac gcaacatacc catatgccca tagcggccca
     2761 tcatcaccac atcttccacc agtacaggaa atgaccagtc aacctcttct gactgaggga
     2821 cgtaggcaac caggtttttc tgtagcgcct gtcgcgtggg aatacccaga acagatatct
     2881 ttccgctggt cagacgcaca aatcccataa tcgctttaaa caacgtcgat tttccggaac
     2941 cgtttacccc aaccagagcg gcaatagatc cacttggtac tgtgaaggat gcatcgcgca
     3001 gtgctgtgtg cccgttacgc caggtgacag taacatcatt aacgacaatg cctgcagatt
     3061 gcatcattat ttctcccttt tcccggcttt aattccctgt accagcgtac tggtagtaac
     3121 cttaagaagg tcgatatacg taggtaccgg gccgttttct gtgctcaggg aatcgacata
     3181 gagcacaccg ccgtagtgtg cgccggtttc acgcgcaacc tgacgcgctg gtttatcgga
     3241 aatcgtactc tcgctaaaga ctgccgggat atgatttttc ttaactatat caacaacctt
     3301 acgtacctgc tgcggtgttc cttgttgatc ggcattaatc ggccacagat aaagctcttt
     3361 tagccccaaa tcgcgtgcga gataagaaaa agccccttca ctggtgacca tccatcgctg
     3421 attctcaggg agttccgtaa tctgcttacg caggggggca agggtttggg taatcttggc
     3481 tttataagta tcggcattac gttggtaggt ttgtgcattt gccggatcgt attttatcaa
     3541 cgcatcacga atattatcga cgtaaatcag agcattatct ggcgacatcc atgcatgggg
     3601 attaggtttg ccctcatagg gtccttcggt gatccctact ggcgtcacac ccgaagagac
     3661 aattacttct ggaaccccat tgagatgctg gtaaaagcgt tggaaccaca attccagatt
     3721 cataccattg gcgagaatca gttgtgcccc ctgcgcacgt ttaatatcgc caggggtagg
     3781 ctgatactca tgaatttctg caccaggctt ggttatggat gagacttctg cagcatctcc
     3841 agccacgttt ttggccatat ccgcgatgat ggtaaatgtt gtaatgacct tgaatttttc
     3901 agttgcactt gcctgaaatg cgatcgagca ggtgagggcg aggcacccca agagcatggt
     3961 tacttttttt attgagtgca taatttagta cctatagtga gcaaaattag aacaaacata
     4021 gcaacggcta tgttttatag cataacccat acaaaatgaa aatgattatc attaacagct
     4081 ttgaggccta aaacagggat taggttgttt cctgctggcg acatgatatt gaggaaaaaa
     4141 gtagagcagc tattaggctt tgaaatgact ttatgaggaa aattgttgat ggcaaaaatg
     4201 gggcaaaacg ctgtagaagg ggcaaaaaag gggcaaaaaa agagtgggtt atcgtagctt
     4261 attgttgtcg ctgataatgt ttaacatatt gaaaaataaa taaaatactt atgggtcaga
     4321 tagttgtgat ttttgccctt acttgttcag gttgtattgt tctttcttac taatttcttg
     4381 attttgcgac atttaaaagc gactcaattc gttatatggc atcagaagag tatgcgtcat
     4441 gccggaacgc ccagcataag aaatctgata taaaactgtg gcgtgtatgg tacggattag
     4501 aggggaaaat gtcagcacat ttgcgaaatg aatcaaaaag cccgcagcaa tgtgcgggcg
     4561 ttagtgtcag cgcacaacca gcacggagca ctctgcgtga cgcactacag ctgcggcgtt
     4621 ggaaccgagc agataagtgg tgatatccgg tcgatgggaa gcaatgatga tcatatgagc
     4681 ggggatcttc ttcgccaatt ccagaatgcg gtctttgggc gagccttcct caacatggac
     4741 atgcactctg tcggttggca gtttaaattt tttaatgatc tcttccagtt gcgatttggc
     4801 ttccgctttc aggtcatcca ttgccggtaa ttctgcggaa tacgctaaac ccagagaggc
     4861 atagtaaggc agtgaaggta ttaccgtcag gaaatgaacc tctgcatcgt caatctttgc
     4921 ctctgcctca acgtggctaa tcacgcgttg agttaattct gaatcggaaa tatcgatagg
     4981 gacaagaatc gttctgttca taaaacctcc tgttttagta tccgcataaa gtgtaacgcc
     5041 agatgacact ttttgtgtaa tgacggagtt cacattttta atttagatca aaggaggaag
     5101 aataagcaga aaaagcccgc cataacagcg ggcaggagga tttagaactg ataaaccaga
     5161 cctaaagcga caatatcatc ggtagagatg ccgttggcag cgtagaaact gtcatcttca
     5221 tccaacaggt tgattttata gtcaacataa gtggacatgt ttttattgaa atagtaagtc
     5281 gcgccaacat cggcgtattt aacaagatct ttatcatcaa cacctgccgg gttgtctgca
     5341 ccatcggcag cgtgcaggtc acggccttta gacatcagga aagagactgc cggacgcaga
     5401 ccaaaatcaa actggtactg tgcagtgact tcaaagttct gggttttgtt tgcaacagca
     5461 taatcgctgt cgccaaacgg ggtcatatta cgcgtttctg aatacatggt tgccaggtaa
     5521 atattgttag catcgtattt tagcccagca gtccacgcgt ctgctttatc accacccgcc
     5581 gcagtatggt taacctggtc attggtgcgg tcagatgagg tgtatgccgc accagcgcta
     5641 aagcccatgc ctaaatcata tgttgtggaa agaccccagc cgtcaccatt ttcatggcga
     5701 acatcacgtc cgttgttggt gccttcctga ccattactgg caccttcgtt gttaccttga
     5761 tactgcaccg cgaagttcag accatttacc agaccgaaga aatcagtatt acgataagtc
     5821 gcgacgccgt tggcacgacc agtcataaag ttgtctgcat tggtataaga gtcaccgcca
     5881 aattcaggca gcatatcggt ccagccttcg atgtcgtaca ttacgccata attacgtccg
     5941 taatcgaaag aaccgtaatc tgcaaatttc agcccggcaa atgccagacg ggtccatgac
     6001 tggttttttg aagattcagt gttgtttgcc tgaatattgt attcccattg accgtagcca
     6061 gtgagttgat cgttaatttg ggtttcgcct ttaaaaccca gacgcgcata gctctgatcg
     6121 ccatctttcg ctgaattatc agaaaaataa tgcaggccat caactttgcc atacagatct
     6181 aatttgttgc cgtctttatt ataaacttcg gctgcatgtg cagcacctgc ggcgagcagg
     6241 gcaggaatta aaagtgccag tactttgctt ttcattgaat aaatccttta gttattttat
     6301 ttgcctttta tccccattcc ggggagtgaa aacatcctaa aggagaagtt cagttagccg
     6361 ataaatatca tttgttacgc cttaagtaaa accttaatca aataattctt ctgaaatgat
     6421 aataagtctg gtgaatgtat cgaagataat
//
LOCUS       DADMKF010000194         6443 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.198, whole genome shotgun sequence.
ACCESSION   DADMKF010000194 DADMKF010000000
VERSION     DADMKF010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6443
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.198"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(135..896)
                     /gene="kduD"
                     /locus_tag="JNC24_003691"
     CDS             complement(135..896)
                     /gene="kduD"
                     /locus_tag="JNC24_003691"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HAY9160343.1"
                     /translation="MILSAFSLEGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIKQVTALGRRFLSLTADLRKIDGIPALLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQRNGGKIINITSMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPIVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(926..1762)
                     /gene="kduI"
                     /locus_tag="JNC24_003692"
     CDS             complement(926..1762)
                     /gene="kduI"
                     /locus_tag="JNC24_003692"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HAY9160344.1"
                     /translation="MDVRQSIHSAHAKTLDTQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFDDMDHVAVKD
                     LR"
     gene            complement(2049..3230)
                     /locus_tag="JNC24_003693"
     CDS             complement(2049..3230)
                     /locus_tag="JNC24_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HAY9160345.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMIQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            3485..4714
                     /locus_tag="JNC24_003694"
     CDS             3485..4714
                     /locus_tag="JNC24_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HAY9160346.1"
                     /translation="MSNIWSKEETLWSFALYGTAVGAGTLFLPIQLGSAGAVVLFITA
                     LVAWPLTYWPHKALCQFILSSKTSACEGITGAVTHYYGKKIGNLITTLYFIAFFVVVL
                     IYAVAITNSLTEQLAKHMVIDLRIRMLVSLGVVLILNLIFLMGRHATIRVMGFLVFPL
                     IAYFLFLSIYLVGSWQPDLLTTQVEFNQNTLHQIWISIPVMVFAFSHTPIISTFAIDR
                     REKYGEHAMDKCKKIMKVAYLIICISVLFFVFSCLLSIPPSYIEAAKEEGVTILSALS
                     MLPNAPAWLSISGIIVAVVAMSKSFLGTYFGVIEGATEVVKTTLQQVGVKKSRAFNRA
                     LSIMLVSLITFIVCCINPNAISMIYAISGPLIAMILFIMPTLSTYLIPALKPWRSIGN
                     LITLIVGILCVSVMFFS"
     gene            5174..5806
                     /locus_tag="JNC24_003695"
     CDS             5174..5806
                     /locus_tag="JNC24_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY9160347.1"
                     /translation="MGLCSRYKSLTCNSCSMHCQIMPEESPRLQYCANSCFCMWPEES
                     SYFNRGVVEGILTKNHNARLSGYIFVDFSVSFLRLFLEKDWIDYLASTDMGIVLVSDR
                     NMQSLANYWRKHNTAISAVIYNDDGLDVANEKIRQLFIGRYLSFTRGNTLTQMEFTIM
                     GYMVSGYNPYQIAEVLDMDIRSIYAYKQRIEKRMGGKINELFIRSHSVQH"
     gene            6140..>6443
                     /locus_tag="JNC24_003696"
     CDS             6140..>6443
                     /locus_tag="JNC24_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAY9160348.1"
                     /translation="MYWIINDNIEFWPEHRKLISVHNADLNVVLTTPASRCLSLLLEA
                     FPDVVAQQDFFTRVWEEEGMRVPTNTLYQNISIIRRGFRAVGDTTHSLIATVPREDS"
BASE COUNT         1808 a         1501 c         1346 g         1788 t
ORIGIN      
        1 agcagataaa attcataaag ttcattaatt gataattgat atggattatt tcataaccat
       61 gatatgaatt atgatgacct acaggtataa aaaaccctgc catgaggcag ggtcataaaa
      121 gtaagaggaa tgaattaacg agccagccaa ccgccatcta cggcaatggt ataaccattc
      181 acataatctg aagcgctgga ggcaaggaac actatcggcc ccatcaggtc actcggcagt
      241 ccccaacgac cagctggaat gcggtcgaga atttccgcgc tacgttgttc atctgcccgt
      301 agttgttgag tattgttggt cgccatgtaa cccggagcta tcgcattaac attaatgttg
      361 tgtttagccc attcgttcgc catcaatcgc gtcacgccca tcacgccgct ttttgatgcg
      421 gtataagaag gcacacggat cccgccctgg aaggagagca ttgacgtgat attgataatc
      481 ttgccgccat tgcgttgcgc gataaagtgt ttcgccgctg cctgagacat gaagaatacg
      541 ctcttgatat tcaggttcat gacatcgtcc cagtcttttt cgctgaactc gagagcatct
      601 tcgcggcgaa tcaatccggc gttattcacc aggatatcaa tatgaccaaa ctcagctacc
      661 gcgcgatcca gcagtgctgg aatgccatca atctttcgca gatcggcggt caggcttaaa
      721 aaacgacgcc ccagcgccgt gacctgcttg atggtttcag tcggttcaac gatgttaatg
      781 ccaacaatgt cacagcccgc ttgcgccagc cccaacgcca tcccctggcc cagtccagta
      841 tcacaaccag tgacgaccgc aactttacct tcgagagaaa atgcacttaa aatcataaca
      901 ataccttatt cgtttatgcc gacaactagc gcaaatcttt aacggccaca tggtccatat
      961 catcaaagac ctggttttca ccaaccatgc cccagataaa ggtataagct ttggttccga
     1021 cgccggaatg gatcgaccag ctgggggaga tcaccgcctg ctcgttatgc atcacaatgt
     1081 gacgcgtttc ttgcggctgc cccatcatgt ggaaaacgca ggcgtcatca tccatattga
     1141 aatagaaata aacttccatc cggcgctcgt gagtgtgaca cggcatggtg ttccacaagt
     1201 tacctggagc cagctccgtc agccccatac tcaattggca ggtttccagc acatccggga
     1261 caaaatattt gttaattgtg cgacggttac tggtgaggtt atcgcctaac gtaactggag
     1321 atacttcgtc cggtgtgact tttttggtgg gatacgtcgt atgcgcgggt gcgcaattgt
     1381 aataaaactt tgccggagtg gcggtatcaa tactggcaaa gacaacttct tttgcacctt
     1441 taccaacata cagggcgtcg cggtgaccga tttcatagca ttggccatcg acagtaatcg
     1501 taccggcacc gccaatattg ataacaccta actcgcgacg ttcaaggaaa tagcttacgc
     1561 cgagttgttt accaacttcc ccgccaacgg aaaccgtttt agttatcggc ataatgccgc
     1621 caacaataat tcggtcaatg tggctgtaaa ccatggtgta ctcatcggcg acaaatacct
     1681 tttcaaccaa aaattcattg cgcagccctt gggtatccag cgtttttgcg tgcgcactgt
     1741 ggatgctctg tcttacgtcc acatcaacct ccgataatca gtgaagcgtg aaaatagaaa
     1801 acgatagtga aacactgttt cgttttctct ataaagggat gatatgccca tgggctaggg
     1861 ttttcaatta aaaataaaac aatgtttcat ttttaagtta aggattaaaa aaagtgcagt
     1921 gtcgatcacg aatatgtcat gagaatggaa aaatgaggga gcccagaaaa tgtatgtcga
     1981 cttgccagcc atcattattc aacagattaa tgacggcaga tatttatcgg taaggaggaa
     2041 atgcagagct actcgtcacg ttcaatggtc aatgccacgc cctggccccc gccgatacaa
     2101 agcgttgcca gccctttgcg ggcattacgt ttcaccattt catgaaccag agaaaccagg
     2161 attcggcaac cggaagcgcc tatcgggtga ccgagtgcga tcgcgccacc attgacattg
     2221 acccgacgct catcccactc aagcatcttg ccaaccgaaa gcgcctgtgc agcaaacgct
     2281 tcattagcct cgataagatc gacatcagcc aactgccagc ctacacgctc caggcaacgg
     2341 cgggtcgcat acaccggcgc aattcccatc aatgccggat ctacaccaac gctggcaaat
     2401 gcgcggatgc gggccagcac gggtaaattc aacgctcgtg ctttggcttc gctcatcatc
     2461 attaccgcag ctgcgccatc gtttatggat gatgcattcc ccgcggtcac agaaccgaga
     2521 ctatcaaatg aaggatttaa acgggctaaa ccttctgcac tggcgtcagt gcgtggctgt
     2581 tcatcggtat caacaaccaa cgtctgaccg ttactttgga tcattaccgg gacgatctca
     2641 tctttaaatc gtccggcgtc aatcgccgct cgcgcttttt gttgcgagct aagtgcgtaa
     2701 gcatcctgca actgacggct gatgccatat tcgcgagcca gattttcggc ggtgacacca
     2761 atatgataat cattgaaggc atcccacaac ccatcatgca ccagactgtc aaccaactgg
     2821 ctattgccaa gctgtgcacc ggtgcggcta tcagtcagaa catgtggtgc gcggctcatg
     2881 ttttcctggc caccggcgat gacaatatca gcctcgccac actgtatcgc ctgagtagcc
     2941 agatgcagtg ctttaagtcc ggaaccgcaa acgtcattaa tcgtgattgc agaaacgcta
     3001 ttaggaagac cacctttaat agccgattgc cttgccggat tctgccctgc ccctgcagtc
     3061 aacacctgac caagaattac ttcatccacc gcatatgcag gaacgccggt acgttctatt
     3121 aacgctttca cgaccagact accaagttcc acggcggaat gacccgctaa cgcaccacga
     3181 aagcagccga taggtgtccg taacgccccg acaatcacaa cgtctttcat cacaacctcg
     3241 caccaattaa cagtgcaata gtaaatgatt gttaattaca gttatcttaa ttgtttaaaa
     3301 aaagtgattt ttatcacaaa ggaaatatgc ctgagcagta gtcagagtca taactggcac
     3361 gtaaggtttg caaccactaa cccaccaata gatgggtaga taaggcgtta atctcccata
     3421 cttaacctgg tttatggtaa attgccctcc attttgttta atttgtagat gatacgttca
     3481 gataatgtct aatatttggt ctaaagaaga aactctgtgg agtttcgcgc tctacggcac
     3541 agccgttggt gcaggcacgc tcttccttcc tattcagtta ggttcggcag gggctgtggt
     3601 cctgtttatt actgctctgg tcgcctggcc tttaacatat tggccacata aagccttatg
     3661 ccagttcatc ctctcatcaa aaacatcagc atgtgaaggg ataacgggcg cggtaacaca
     3721 ctactatggc aagaagattg gtaatctgat taccacgctg tacttcatcg ccttttttgt
     3781 cgtcgtgttg atatatgcag tggcaattac caactcactt acggaacagc tggcaaagca
     3841 tatggttatt gatcttcgca tccgtatgtt ggtgagtctg ggtgttgtat taattctgaa
     3901 tctcattttt ctgatgggac gtcatgccac tattcgggta atgggatttt tggtattccc
     3961 attgattgcc tatttcttat ttctttccat ttaccttgtc ggtagttggc aacctgatct
     4021 attaacgacc caggtagagt tcaatcagaa tacccttcac cagatatgga tatcgattcc
     4081 cgtgatggtt ttcgccttta gccatacgcc cattatttct acgtttgcca tagacagacg
     4141 tgaaaaatat ggcgaacacg ctatggataa atgcaaaaaa attatgaaag tcgcttatct
     4201 catcatctgc ataagtgtac tgttctttgt ctttagctgc ctgctttcta ttccaccttc
     4261 gtatattgaa gcggctaaag aagaaggggt caccatttta tcggcgcttt ctatgctgcc
     4321 gaacgcccca gcatggttgt caatttccgg gattattgtc gcagtagttg cgatgtcgaa
     4381 atcattcctg ggtacgtact ttggcgttat tgaaggtgcc acggaggtcg tcaaaacaac
     4441 actacagcag gttggtgtaa agaaaagtcg tgcatttaac cgcgcactat caattatgtt
     4501 ggtatcgctg attaccttca ttgtttgttg cattaacccg aacgcgattt cgatgattta
     4561 tgcgatcagc ggcccgctca ttgccatgat acttttcatc atgcctacgt tgtcaacgta
     4621 tctgatcccg gcgcttaaac cctggcgctc catcggaaat ctgattacgc tgatcgtggg
     4681 tatcctgtgc gtatcggtaa tgttctttag ctaatatccg ttgtggatgg ttcaaaattg
     4741 ctacaatttt gaaccattcc atttttcata atattcattt gttattattc cgccctatca
     4801 cttctctcgt catatcctag aattatcagt tagttaaaca cacaaagaga tctgaacatc
     4861 ttaaataaga ctatttaaga tgcataactt agattcgcaa gatatcttct ggtcattcaa
     4921 aaacaatttg cgatatagat attctcatac catgagatag ttatctggac cacgttcgac
     4981 catgcaagat taaatcagtg aaacataatc atatttaata acgagaaatg catttttaaa
     5041 tgtcttaatc agaattatag caaatacaga ttaatcgcaa tttttcccag caaaattaca
     5101 aaatcaagat gtattgacat catcaatcac caatgaaatt atgcaattat atacggatag
     5161 ggaggttctt aacatggggc tttgtagtcg ttataaaagt cttacatgca atagttgctc
     5221 aatgcattgc caaataatgc cagaagagtc accgcgtttg cagtattgcg caaactcgtg
     5281 tttttgtatg tggcccgaag aaagttcata ttttaatcgt ggtgtggtgg aaggtatctt
     5341 aacaaaaaac cacaatgcga gattaagcgg atatattttt gtggattttt cagtaagttt
     5401 tttacgtcta ttccttgaaa aagactggat tgactatctt gcaagtactg acatggggat
     5461 agttttagtc agcgaccgca atatgcaatc gttagctaac tactggcgaa aacacaacac
     5521 cgcaatatct gctgtcatat ataatgatga tggtcttgat gttgccaatg aaaagatcag
     5581 acaactattt attggtcgtt atttatcatt taccagaggt aacacattaa ctcagatgga
     5641 attcaccata atggggtata tggtttctgg ttataatcca tatcagattg ctgaagtgct
     5701 ggatatggat atccgtagca tctacgcgta caagcaacga atcgaaaaga gaatgggtgg
     5761 taaaataaac gaattattta ttcgttcaca ttcggtccaa cattgatact caacgaccag
     5821 ccagaatcat actctgttca tacgggcatc tattgaaatg cccgtatttt ataaataagc
     5881 agtaacccca agagtaaaca acatagtatt taaaaccatc acacgaaaaa ttcagaagca
     5941 ttacgaaata tgaatttaca aacaatagtg gcataaatgt taaccatgtt aatttacgta
     6001 aagttttacg ttgcaacatt aaagcctcat ttcaatcatc atgataaata taaaattaat
     6061 atatatttat gccgtaaata ccaatatact tagcaaacta tgtgatctcc atttcgattg
     6121 atttagtgtt tattgacgta tgtactggat tattaacgat aatatcgagt tctggcctga
     6181 gcaccgaaaa ttaatatcgg tacataacgc cgatcttaac gtcgttctga caacgccagc
     6241 cagtcgatgt ttatcacttc tacttgaagc ttttcctgat gtggttgcac aacaagattt
     6301 tttcaccaga gtctgggaag aagaaggtat gcgtgtgcct actaacacgt tatatcagaa
     6361 catatccatt atcagacgcg gatttcgcgc tgttggtgat actacccact cgctaattgc
     6421 aaccgtgccg agagaggatt caa
//
LOCUS       DADMKF010000195         6410 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.199, whole genome shotgun sequence.
ACCESSION   DADMKF010000195 DADMKF010000000
VERSION     DADMKF010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6410
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.199"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            79..319
                     /locus_tag="JNC24_003697"
                     /pseudo
     CDS             79..319
                     /locus_tag="JNC24_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001450234.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(370..1761)
                     /locus_tag="JNC24_003698"
     CDS             complement(370..1761)
                     /locus_tag="JNC24_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HAY9160349.1"
                     /translation="MNFPLIANIVVFVVLLFALAQTRHKQWSLAKKVLVGLVMGVVFG
                     LALHTIYGSDSQVLKDSVQWFNIVGNGYVQLLQMIVMPLVFASILSAVARLHNASQLG
                     KISFLTIGTLLFTTLIAALVGVLVTNLFGLTAEGLVQGGAETARLNAIESNYVGKVSD
                     LSVPQQVLSFIPKNPFADLTGANPTSIISVVIFAAFLGVAALKLLKDDAPKGERVLAA
                     IDTLQSWVMKLVRLVMQLTPYGILALMTKVVAGSNLQDIIKLGSFVVASYLGLLIMFA
                     VHGILLGINGVSPLKYFRKVWPVLTFAFTSRSSAASIPLNVEAQTRRLGVPESIASFA
                     ASFGATIGQNGCAGLYPAMLAVMVAPTVGINPLDPMWIATLVGIVTVSSAGVAGVGGG
                     ATFAALIVLPAMGLPVTLVALLISVEPLIDMGRTALNVSGSMTAGTLTSQWLKQTDKA
                     ILDSEDDAELAHR"
     gene            complement(1894..2484)
                     /locus_tag="JNC24_003699"
     CDS             complement(1894..2484)
                     /locus_tag="JNC24_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAY9160350.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPEGWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            complement(2647..3315)
                     /gene="hxpB"
                     /locus_tag="JNC24_003700"
     CDS             complement(2647..3315)
                     /gene="hxpB"
                     /locus_tag="JNC24_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HAY9160351.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLRDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            3462..3998
                     /locus_tag="JNC24_003701"
     CDS             3462..3998
                     /locus_tag="JNC24_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9160352.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFTHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            complement(4039..4899)
                     /locus_tag="JNC24_003702"
     CDS             complement(4039..4899)
                     /locus_tag="JNC24_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HAY9160353.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQLQPSLLHGDLWSGNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(5005..5295)
                     /gene="ghoS"
                     /locus_tag="JNC24_003703"
     CDS             complement(5005..5295)
                     /gene="ghoS"
                     /locus_tag="JNC24_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HAY9160354.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            complement(5396..6325)
                     /gene="pfkB"
                     /locus_tag="JNC24_003704"
     CDS             complement(5396..6325)
                     /gene="pfkB"
                     /locus_tag="JNC24_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416237.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HAY9160355.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISASQKQG
                     IRCIVDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
BASE COUNT         1662 a         1599 c         1566 g         1583 t
ORIGIN      
        1 atatacatcc tgccgggagc atttttgctg cgattcaaaa aaaatgagag tctcctaaag
       61 tttccttcga taatatcaat gcttaatatc aatcttcttt ttaatccact aaatcttccg
      121 ggaatgggaa gtggtgtgct tgaagatatt atgagtattc cagatagttc acttagaaaa
      181 cgtctcgggt atgaggtttt aagcttctca ctacaggcac attcattgag ccaggaatgt
      241 attgataagt tggacatttt tttcgctgac gatctgttta aatatgagtc agtatgcatc
      301 gccgcgatgg aacatttaaa aagtaaagca acggccccta tacaaaacgg accgttgcca
      361 gcataagaat taacggtgtg ccagttcggc gtcgtcttcg ctatccagaa tggctttatc
      421 ggtttgcttc agccactggc tggtcagcgt gccagctgtc atcgagccac taacgtttag
      481 cgccgtacgg cccatgtcga taagcggttc aacggagatt aacagcgcca ccagggttac
      541 tggcaggccc atcgcaggca gcacaatcag tgcggcgaaa gttgcaccac caccgacacc
      601 ggcaacgcct gcggaactaa cggtaacaat accgaccagc gtcgcaatcc acatcgggtc
      661 cagcgggtta atgccaaccg taggcgcaac catcaccgcc agcattgccg gatacaaacc
      721 ggcgcagccg ttctgaccaa tggttgcacc gaaagaggcg gcgaaactgg cgatggattc
      781 aggaacgccc agacgacgcg tttgtgcttc cacattcagt gggatagacg cagcactgga
      841 acggctggta aaggcaaacg tcagcacagg ccatacctta cggaagtact tcagcggact
      901 cacgccatta atgcccagca gaatgccatg cactgcaaac ataatcagca gaccgaggta
      961 ggacgcgaca acgaaacttc ccagtttgat gatgtcttgc aagttagaac ctgcaaccac
     1021 tttggtcatt agagccagaa tgccgtaagg ggtcaactgc atgacaaggc gaaccagttt
     1081 catcacccag ctttgtaggg tatcgatagc ggctaagacg cgttcacctt tcggcgcatc
     1141 atccttcagc agtttcaatg cagctacgcc gaggaatgcg gcaaaaatta ccacgctgat
     1201 aattgacgtc ggattggctc cggtaagatc ggcaaacggg tttttcggga taaaggacaa
     1261 gacctgctgc ggaacgctca gatcagagac tttaccaaca tagttacttt caatggcgtt
     1321 cagacgtgca gtttctgcac caccctgaac cagaccttca gccgtcaaac caaacaggtt
     1381 ggtgaccagc acaccgacca gcgccgcaat cagcgtggta aacaaaagcg taccgatggt
     1441 cagaaaactg attttgccta actgagatgc gttatgcaga cgggcaaccg cgctcagaat
     1501 agaggcgaag actaacggca taacgatcat ttgcagcagt tgaacatagc cgttaccaac
     1561 gatgttaaac cactgtacag aatctttaag tacctggctg tcagaaccat aaatggtatg
     1621 cagggcaagg ccaaaaacca cacccatcac cagacccacc agcacttttt tcgccagact
     1681 ccactgttta tggcgggtct gagccagcgc aaacagcagt acaacgaaca ccacgatgtt
     1741 cgcaattaat ggaaagttca tccccgttct cctgatctta ttatttctat cactgcgtag
     1801 cagtcatagt gttggcgcaa gattaacaga actgtgagct ggctcttata ttcaaatgga
     1861 attgcttatt ccagaaaggt gatttttgcc tttttagtat tcaacctgat gcttaataag
     1921 ccgatgaaac tggatctgca aggtattgat catttgcgat gaccaacgaa cagcgctgtt
     1981 ctcgggtaat gaatggggca tccataccga ccagacaaat aatgccatgc agataaaacg
     2041 ttccagttgg ttgccctttt ggggaaccag gataggcaag cggaaacgcc agcggcatgg
     2101 ccagagcagg ggaacaccgg cgggtgtcag catatcggca agtatgtggc tcaaataacc
     2161 cagcaccatt ccttgtagcg catcagccgg aatgaaccag ccttccggaa ccttaaggta
     2221 aaaggttgcc agcagcgcaa ataccgccag cagactgtgg gtaaaaccac gatgcccaaa
     2281 agcgcgggcg atcggttttg atatccattt taatcgctgc ccaagaaacg actttgggtg
     2341 atcgatgtcc ggtaacaaac acgtcaggat tgcggaaggg acaatatgcc accagtcacc
     2401 ctgtgccagc acgggcgtca gctcggcatt tttggcaaat accgcacaag caatagaaaa
     2461 gagaaggtga ccttccgccg tcatgataaa actcactaaa ctgtcgatac gtacagtata
     2521 ggatttttat acagtgcacg gaagtggtga cggtgtaact ctttctctta aattgacgat
     2581 taatccgtca gttgaatgca aattcagata aagaaccagg ggcagcgtga tcactgcccc
     2641 tggtcattaa ccgagaaggt cttttgcggt gagttctgtc agcgatgaga gtttgacgtt
     2701 tgctaataca aaacgtggat cattttgcgc ttctggcgca ggaacgacga tggaacgcat
     2761 gcgggctgct ttagaagcga tcatgccatt taccgagtct tccagcgcca cgcaggtcag
     2821 tgggtcaacg cccagttttg ctgcgcagtc gagatatact tgcggatgcg gcttgctgta
     2881 aggcagtttt tcagccgagg cgagggcatc gaaactgtcg cgtaagtcaa acatggtcaa
     2941 cactttttcc agcatatgta gtggtgacgc ggaggccagt cccaccaata aaccttgttc
     3001 tttgcataac gcaacagctt cgcgcacgcc tggtaataat ggacgtgtct cttcaaccag
     3061 tgaaatggca cgggcaataa cccgttctac tacttcctga cggcttggcc cattccacgg
     3121 ttgccgggcg taccaaagat cgaccaccat atcgatgcgt aaacctaagg tgtccggcag
     3181 ctcgttacga cgggagatat ccacccccag gcttgccatc acatccagtt cggctcgatc
     3241 ccgtaaaggt tctgagtcga taagtaatcc atccatatca aaaattgcag caagaatctg
     3301 acgcggggtt gacattacaa cctctccttt agagcattat gtgtaaggta ttaagccgtg
     3361 cttatgatta agcgcctatc atatcgcctt taaagaatgg gcgaaaacag atatctacag
     3421 gtaaactaag tgaaacgacg cgtcttaatc aaggagaact catgacgtat caacaagctg
     3481 gacgcattgc tgttttgaaa aggattttgg gttgggtgat ttttatacct gcattgatct
     3541 ccacattgat ttctttgctg aaatttatga atacccggca ggaaaaccag gaaggcatta
     3601 atgcggtcat gctcgacttt actcatgtca tgatcgatat gatgcaggcg aatacgcctt
     3661 ttttgaatct gttctggtat aactccccga cccctaattt taatggtggc gtgaacgtta
     3721 tgttctgggt gattttcatc ctgatttttg tcggactggc gttgcaggat tccggtgccc
     3781 ggatgagtcg ccaggcgcgc ttcttgcggg aaggtgttga agatcaactc attctggaaa
     3841 aagccaaagg ggaagagggg ctaacgcgtg aacaaattga gtcccgtatt gttgttccgc
     3901 accatactat tttcctgcag tttttctcac tatatatcct gccggttatt tgtattgctg
     3961 caggttatgt gttcttttct ctgcttgggt ttatttaatc aaaaggccgc acggagcggc
     4021 cattatcctc aacccatatc atgctgctaa taatctatcc aatgactgct gagcaataac
     4081 caaatgctga ccgccaaata atcttgcacg atttaacagc gtgtagagtt ggtaaaccgg
     4141 ttgacgttca aggaaatcgg caggtagcgg ggatactgac tgatagccgt catagatttg
     4201 tggcggctgt tcagtatgca gcggtaacat cgccaggtcg cactctcggt caccccagta
     4261 gcaggcaggg tcgaaaatgt acgggccatc cggacccagc gcacagttgc cggaccataa
     4321 atcgccgtgc aacagagaag gctgcagttg atgcgaggcc agacgctgct gaatatgctc
     4381 gacgatggcg tcgatattgc cgaaagcgat ccctttctct gctgccagtt ccaactgcca
     4441 gccaatccgt tgttcagcaa aaaacgttga ccagcgacgt tgccaggtgt tgggctgtgg
     4501 agttgtggag agcgagttat caaaatcgag gccaaattgt ggttggtcac tccattgatg
     4561 taaacgcgca atttgctgac caagaataaa tgcgctatgc gcatccagcg gacgaggtgg
     4621 gagataatcc atcaccagaa aactgtagtc acggtcagcg cctactgccc aaaccttagg
     4681 cacggtgacg gttttactac gcgacagtaa ctccagttgg tcggcttcgg cggtaaaacc
     4741 gggaagcagt tccctttcat cacatttgac gaaaaagtca tgtcctgcat agcgcaaatg
     4801 ccatgcggca tggacttctc cgccaggcag ttcattacgc agttcgattt cgccttcacc
     4861 taactgctcg ctcaaaagac gactgattgc ctgccacatg atgtctctcc catgttgtct
     4921 gccagatcat aaggttagcg caagaatgcg gtgtaaaaac acgagctaac gcacaccaca
     4981 gactgattat tgcccaaaaa ccatttattt tctgttgcta ttccattcct cccaagtcgt
     5041 gatggtgatt tcaggatctt tgccggttgc actttgggta agccccgaaa ccagttctct
     5101 aatttcttct tcactttggg tactgataag tccaaaagtg ttagtcccca gctcatggat
     5161 atttccctca tcatccgtca tggtgagcaa aaaaccgtcg cgagtcaggt aattattcaa
     5221 ctcgttaatt tcagtcaatg tatcctcatg caacatgacg gttatgacat aacggacaag
     5281 atcgccactg gccatagttc acccctttgt tatcacatga cttttgagca aagtcggaga
     5341 aacgcgttgc cgacaggttg gtggtgattc ccccaatgct gggggaatgt ttttgttagc
     5401 gggaaaggta agcgtaaatt ttttgcgtat cgtcatggga gcacagacgt gttccctgat
     5461 tgagtgtggc tgcactcccc gcagctacgc caaaacggac catctcttca agagaggcat
     5521 tttctgccag tttcagtgtc atcgcgccga ccatgctgtc accagcgcca acggtactct
     5581 ggcttttcac cggtggtggc accacctgaa tgcagttttc actatcaaca cccagcgctc
     5641 cttgtggacc aagggaaacg acaacccgtt tggccttgcc gctattaacg atttcctgcg
     5701 cggctttgcg gacatcgtcc ggctgggtga gttcgcgatt caccagcgca ctgagttctt
     5761 tttggttagg cttaaccaac tcgatgttac caattgccag tgctgcactt aacgcttcgc
     5821 cagaactgtc gacgatgcag cggatccctt gtttttgcga agcggaaatc agttgggtta
     5881 atttttccag cttcacacct ggcggcaggc ttccgcttat gaccaggatg gccccggatt
     5941 caatttccag aacttgctct tcaagctggc gaaactcatc ttcatttaat gccgcgcctg
     6001 gcataacaaa acgatactgc tcaccgcttg cttccacatg tacatgtaaa ttctgccggg
     6061 tccagtcttt ggcttctaca gtagcgacgg ggacattttc atccgccaac agtgaaacca
     6121 ggtgttcgcc ggtcgcgcca cccgccggga agatcgctgt ggcactgcct ccaagatggg
     6181 caatggcgcg ggcgacgttg atgccgccgc ccccgggttc gaacaccggt gcggtacagc
     6241 gcagttttcc ttcgggataa atttgcgggg taattgttgc gctatcgaga gagggcgcaa
     6301 gtgtcaacgt atagatacgt accatcattt cctcctatag gctgatttca gtctggcacc
     6361 gaatcagcgg aaagtgaaga aattaacaat atgatttatt ggagctttat
//
LOCUS       DADMKF010000196         6352 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.200, whole genome shotgun sequence.
ACCESSION   DADMKF010000196 DADMKF010000000
VERSION     DADMKF010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6352
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.200"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            191..2038
                     /gene="ldtD"
                     /locus_tag="JNC24_003705"
     CDS             191..2038
                     /gene="ldtD"
                     /locus_tag="JNC24_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HAY9160356.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAVFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPVKGTRWLYSSKPYALATPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLVYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWQVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADGRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            2219..2767
                     /locus_tag="JNC24_003706"
     CDS             2219..2767
                     /locus_tag="JNC24_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbK family protein"
                     /protein_id="HAY9160357.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            2794..3441
                     /gene="gloC"
                     /locus_tag="JNC24_003707"
     CDS             2794..3441
                     /gene="gloC"
                     /locus_tag="JNC24_003707"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HAY9160358.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(3663..4853)
                     /locus_tag="JNC24_003708"
     CDS             complement(3663..4853)
                     /locus_tag="JNC24_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/tyrosine/aromatic aminotransferase"
                     /protein_id="HAY9160359.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSSLINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSETVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCEAI
                     VAVL"
     gene            complement(5038..6126)
                     /gene="ompF"
                     /locus_tag="JNC24_003709"
     CDS             complement(5038..6126)
                     /gene="ompF"
                     /locus_tag="JNC24_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HAY9160360.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVELVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
BASE COUNT         1597 a         1558 c         1610 g         1587 t
ORIGIN      
        1 ttacggcgcg cttttcttta aacttcttta cattaggtta tgtaaaaacg tatcggcgtt
       61 tatatactga agataagcct gatgagtaac aggcttgctc gtcatacttt cgtgagtatt
      121 ggcgttgtac aggcaagtcg taaaataaca gcctggctat tcagagtatg ataaaaacag
      181 ggggcaaggg atgttgctta atatgatgtg tggtcgtcgg ctgtcggcaa tcagtttgtg
      241 cctggccgta acattcgctc cactgttcaa tgcgcaggcc gatgagcctg aagtaatccc
      301 tggcgacagc ccggtggctg tcagtgaaca gggcgaggca ctgccgcagg cgcaagccac
      361 ggcaataatg gcggggatcc agccattgcc tgaaggtgcg gcagaaaaag cccgcacgca
      421 aatcgaatct caattacccg caggttacaa gccggtttat cttaaccagc ttcaactgtt
      481 gtatgccgca cgcgatatgc aacccatgtg ggaaaaccgt gatgctgtta aagccttcca
      541 gcaacagctg gcagaggtgg cgattgccgt tttccagccg cagtttaata aatgggtaga
      601 gttactgacc gatcctggtg ttaacgggat ggcacgcgac gtagtgctct ctgatgcgat
      661 gatgggctat ctccatttca ttgcaaatat tccggtcaaa ggcactcgct ggctatatag
      721 cagtaaacct tatgcacttg caacgccgcc gctctcggtg attaaccaat ggcagctggc
      781 gctggataaa ggtcaattgc ctacgtttgt tgcaggactg gcaccgcagc atccgcaata
      841 tgcggcgatg catgaatcgt tactggcctt actcagtgac accaaaccgt ggccccaact
      901 gaccggcaaa gcaacgttgc gcccagggca gtggagtaac gacgtaccgg cgttgcgcga
      961 aatattgcaa cgcacaggca tgttggacgg ggggccgaaa attactctac ctggcgatga
     1021 cacgccaact gacgcggtag tcagcccatc cgctgttact gttgaaacag cagaaactaa
     1081 gccgatggac aagcaaacga cgtctcgtag taaacctgcg cctgccgttc gcgccgccta
     1141 cgataatgaa ctggtggaag ccgttaaacg ttttcaggca tggcaaggat tgggggcaga
     1201 tggtgctatt ggcccggcaa cacgtgactg gttaaacgta acgcccgccc agcgtgctgg
     1261 cgtgttggct ctcaacatcc agcgattgcg cttgctgcca acagagcttt ctaccgggat
     1321 catggttaac attccggcct attcgctggt ctactatcag aacggcaatc aggtgctgga
     1381 ttcgcgagtc attgtcggtc gccccgatcg caaaacgccg atgatgagca gtgcccttaa
     1441 caacgtagtg gtaaacccgc cgtggaacgt acctccaact ctggcacgca aagatattct
     1501 gccaaaagtg cgcaacgatc cgggatatct cgaaagccat ggctatacgg tgatgcgcgg
     1561 ctggaacagc agagaagcga ttgacccatg gcaggttgac tggtctacaa tcacggcctc
     1621 gaatttaccg ttccgcttcc agcaggctcc aggcccacgg aactcgctgg ggcgctataa
     1681 attcaatatg ccgagttcag aggccattta tttgcatgac acgccgaacc acaatctgtt
     1741 caagcgtgat acacgcgcat tgagctcagg ctgtgtacga gtgaataaag cttccgatct
     1801 ggcgaatatg ctgttgcagg atgcaggctg gaatgacaaa cgtatttctg atgcgctgaa
     1861 gcagggtgat acacgttacg tcaatattcg gcagtcgatt ccggtgaatc tctactacct
     1921 gacggccttt gttggtgcag atggtcgtac ccagtatcgt acagatattt acaattatga
     1981 tctgcctgcg cgatccagct cgcaaatcgt atcgaaagcg gaacaattaa tcaggtaaat
     2041 gaagtagttc ggggaaatca gttgtcgtaa taattagcat gattgggggc gattctctgc
     2101 agcccccgtc actgctgggg ttgagtcatc ttgacgtctg ctttacgggc ggttaaggtg
     2161 cctcttgtgc gccagaagtg catataaacg ataacattga cctgtagact tgattatcat
     2221 ggacaaattc gacgctaatc gccgcaaatt gctggcgctt ggtggcgttg cactcggtgc
     2281 cgccatcctg ccgacccctg cgtttgcaac actctctacc ccacgcccgc gcattttgac
     2341 actcaataat cttcataccg gagagtcaat caaagcggag tttttcgatg gcagaggcta
     2401 tattcaggaa gaattggcaa aacttaacca ttttttccgc gattaccgcg cgaacaaaat
     2461 aaagtccatc gacccaggat tattcgacca gttgtatcgc ctgcaagggt tgttaggcac
     2521 gcgcaaaccg gtgcaactca tttccggtta tcgttctatt gataccaaca atgaactacg
     2581 cgcccgcagc cgtggagtag cgaagaaaag ctatcacact aaaggccagg cgatggattt
     2641 ccatattgaa ggtatcgcgt taagcaatat tcgcaaagcc gcgttatcta tgcgcgcagg
     2701 tggtgtagga tattatccac gtagtaactt tgtgcatatt gataccgggc cagcacggca
     2761 ctggtagtaa ttgcttaacg aaacaggggc agtatgaact atcgtattat tccggtcacc
     2821 gcattctccc agaactgttc attaatctgg tgtgaacaaa cccgtctggc cgcactggtc
     2881 gatcctggcg gcgatgcgga aaaaatcaaa caggaagttg atgacagcgg cctgacactg
     2941 atgcagatcc tgctgacgca tggtcatctg gaccacgttg gcgcagcggc ggaactggcg
     3001 caacattacg gcgtgccggt ttttggcccg gaaaaagaag atgagttctg gctgcaaggc
     3061 ttgcctgcgc aaagtcgtat gtttggtctg gaagagtgcc agccgctgac gccagatcgt
     3121 tggctgaacg aaggcgatac catcagcata gggaatgtga ctttacaggt gttacattgc
     3181 cctgggcata cgccgggtca tgtcgtgttt tttgatgatc gggcaaagct gctgatttct
     3241 ggcgatgtta ttttcaaagg cggagtaggg cgcagtgact tcccgcgtgg cgatcataat
     3301 caactgattt cttcaatcaa agataaattg ctgccactgg gggatgacgt gacatttatt
     3361 ccgggtcacg gaccattatc cacacttggt tatgaacgcc tgcataatcc cttcctgcaa
     3421 gacgaaatgc ccgtctggta aggcacataa aaaagcccgc ttttaatgcg ggcctggatt
     3481 tctggcaaag tgcgctttgt ttatgccgga tgcggcacga gcgccttatc cggcctacaa
     3541 aatcgtgcaa attcaaaata ttgcagggga cgcgtaggcc tgataagcgt agcgcatcag
     3601 gcaatgttgc gtttgtcatc agtctcagcc cgcttttcag cgggcttcat tgtttttaat
     3661 gcttacagca ctgccacaat cgcttcgcac agcggagcca tgttatctgg tgtcatcccg
     3721 gccacattta cgcgaccaga agcaaccgca tatacgccaa actcttcgcg cagacgcagc
     3781 acttgttctt ttgtcaggcc actgaaggag aacatgccgt tctgtttgat gataaagctg
     3841 aagtcgcggt ttgcgccttt ttcctgcagc gtattgacga acaactgacg catacgctga
     3901 atacgctggc gcatatcagt cagctcttgt tcccaaatcg cacgtaacgc atcgttgctc
     3961 aggatggtgg caacaacaga agcgccgtgt gctggtgggt tagagtagtt agcgcgaatc
     4021 gccgctttca tttggctgaa tgcgcgatca acggtttcac tgtcggcagc aaccagagta
     4081 caagcgccaa cacgctcgtt gtacaggcca aagtttttag agtaggaact ggcaacaatc
     4141 agctctttat gcatagccgc gaaagcgcgc agtccttcag catcttcttc cagaccacgg
     4201 gcaaaaccct ggtaagcgaa gtcaaacagc ggtaaccagc ctttctcaac ggagagttgt
     4261 gccagtgtct gccattgttc cagcgtaggg tcgataccgg ttgggttatg gcagcagcca
     4321 tggaacagca ctacgtcgcc agcctgagct tcattcaggc tgttaatcag tgcatcgaag
     4381 tcaagagtgt gattttccgc atcataataa gcgtattcac gaacttccag acctgcagag
     4441 ttaaagacgc tcttatggtt cggccagctt gggttgctca cccacactcg cttaacgctg
     4501 gtattttttg ccaggaaatc ggcagctacg cgtagtgcgc cagtgccccc cggagtctgt
     4561 gccgtgcgag cacgtttgtc attgatcagg gagctacctt taccaaacag cagttcctga
     4621 gtgcagcgac caaattcagg gatgccgtca atgccgaggt aatttttggt ggtttcattt
     4681 tcgagcagat actgttcagc ctttttcacg ctggtcagta ccggggtttt gcccgtctca
     4741 tctttataga caccaatccc gaggttaatt ttgccgggac gttcatcggc acgaaacaga
     4801 tcggccaggc ccagaatcgg gtcggcagga gcggcggtaa tgttctcaaa catgacgagg
     4861 ttccattatg gttacagaag ggaagtccgc tatcagggta acgggagatt tacaaaattc
     4921 caactattac tgatgaaaac gcaggctgtt tttgcaagac gtgagattgc tctggaaggt
     4981 ataaaaaaaa caggaccaaa gtcctgtttt ttcggcattt aacaaagagg tgtgctatta
     5041 gaactggtaa acgataccca cagcaacggt gtcgtctgaa cctacgccca gtttgttgtc
     5101 agaatcgatc tggttgatga tgtagtcaac ataggtggac atgtttttgt tgaagtagta
     5161 ggttgcgccc acttcaaagt agttcaccag ctcaacatca ccgatacctt ctacgtcttt
     5221 cgctttagat ttggtgtaag cgatggacgg acgcagaccg aaatcgaact ggtattgcgc
     5281 aactaacaga acgtcttgcg ttttgttggc gaagccgctg gtgtttgtaa atttattagt
     5341 gatcggcgta gcgttacggg tttcaccgta gttcgctgcc aggtagatgt tgttcgcgtc
     5401 gtacttcaga ccagtagccc actgttcagc ttttttaccg ttgccaagag gttgagcttc
     5461 ttgcaggttg gtacggtcag ctgcaccata agcaccaacg ataccaaagc cttcgtattc
     5521 gtagctgata gaaccgccaa caccgtcgcc gttagaacgg cgtgcagtgt cacgctcgtt
     5581 tttacccagg tactgaacag cgaagttcag gccatcaacc agaccaaaga agttggagtt
     5641 acgataggta gcaacgccgc caacacgacc aacgaagaag tcatcgctgt atgcagtatc
     5701 accaccaaat tctggcagca tatcggtgta acccagtgca tcataaacca caccgtagtt
     5761 acggccgtaa tcgaaagaac caacgtcagc gtatttaaga cccgcgaatg ccagacgcgt
     5821 tttgttacca gtttgagcgt cagcgccttc agagttgtta ccctggaagt tatattccca
     5881 ctgaccataa ccggtcagat cggaattgat ttgagtttcc cctttaaaac caagacgggc
     5941 ataggtcatg tcgccattgc caccgtaact gttttcaccg ttacccttgg aaaaataatg
     6001 cagaccaaca gctttaccgt acagatctac tttgttgcca tctttgttat agatttctgc
     6061 agcgtttgca gtacctgcta ctaacagagc agggacgatc actgccagaa tattgcgctt
     6121 catcattatt tattaccctc atggtttttt ttatgacacc tgccactgcc gtcaataagt
     6181 tctgtcaata aaaatttacg gaactattga tgagagtttg gtgtctttat gtgtctgcag
     6241 gcatctttcc attcaaacta acgtttcgct accgtgaaag tgctacaaag ataaagattt
     6301 agtttcaaaa agaaaaaata tgtaaccaaa agtaaaaatt aaggaacttt gt
//
LOCUS       DADMKF010000197         6260 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.201, whole genome shotgun sequence.
ACCESSION   DADMKF010000197 DADMKF010000000
VERSION     DADMKF010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6260
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.201"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(83..634)
                     /locus_tag="JNC24_003710"
     CDS             complement(83..634)
                     /locus_tag="JNC24_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAY9160361.1"
                     /translation="MAILGYCRVSTDDQSITNQQMQIEEAGYNIAKWFADEAVSGSVK
                     ASLRNGFSSLLAYAREGDTVVVVAVDRLGRDTIDVLSTVKALQAKGVTVISLREGFDL
                     SSAMGEAMLGIMSTLAQLERSLIAERRKAGIERAKAEGVHMGRPVKASSEAVQMLISQ
                     GKTRLQIQEELGISRATYYRLAK"
     gene            1221..1568
                     /locus_tag="JNC24_003711"
     CDS             1221..1568
                     /locus_tag="JNC24_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005069111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HAY9160362.1"
                     /translation="MILDSRPVHAARPHSEAIRDAQRKKPKVPVHAVLTATNPLIRFI
                     GSDDMTQNRELFQVWLQKLAQWHQTTTPYLFLHTPDIAQAPELVHTLWEDLRKTLPEI
                     GAVPAIPQQSSLF"
     gene            1621..2016
                     /locus_tag="JNC24_003712"
     CDS             1621..2016
                     /locus_tag="JNC24_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9160363.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            2057..2800
                     /gene="cmoA"
                     /locus_tag="JNC24_003713"
     CDS             2057..2800
                     /gene="cmoA"
                     /locus_tag="JNC24_003713"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HAY9160364.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCSRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            2797..3768
                     /gene="cmoB"
                     /locus_tag="JNC24_003714"
     CDS             2797..3768
                     /gene="cmoB"
                     /locus_tag="JNC24_003714"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HAY9160365.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     AVEFLPEIKPYRLDLLHSVTAESEEPLSTGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIVDVC
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(3933..6224)
                     /locus_tag="JNC24_003715"
     CDS             complement(3933..6224)
                     /locus_tag="JNC24_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001433402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin guanine dinucleotide-containing
                     S/N-oxide reductase"
                     /protein_id="HAY9160366.1"
                     /translation="MNVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTTRIQHPMV
                     RKSYLDNPLQPTKGRGEDTYVQVSWEQALKLIHEQHDRIRKANGPSAIFAGSYGWRSS
                     GVLHKAQTLLQRYMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQTSWPLILEN
                     SQVVVLWGMNPLNTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIDFFGDNA
                     TWIAPNMGTDVALMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLRGKSDNTPKSAA
                     WAAEITGVPEAQIVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGT
                     PGGGFGFSYHYSNGGNPTRVGGVLPEMSAAIAGQASEAADDGGMRAIPVARIVDALEN
                     PGGKYQHNGKEQTYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAA
                     KHADIVLPITTSFERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFADLAELLKP
                     GGKEIYTEGKDEMAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQY
                     VRYGDFRADPVKNALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGTADEKQL
                     QLLTAHPAHRLHSQLNYAELRKKYAVADREPITIHTEDAARFSIANGDLVRVWNKRGQ
                     ILTGAVVTDGIKKGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQLANACAGNSAL
                     VYIEKYTGNAPKLTAFDKPAVQA"
BASE COUNT         1474 a         1605 c         1450 g         1731 t
ORIGIN      
        1 tttagtaccc gtcacaccag ctatgtgacc cccttggcaa aaaagccaaa tatcagctac
       61 atccatgtaa ctattcagta acttatttag caagtctgta ataagttgct ctagagattc
      121 ctagctcttc ctgaatctgc aacctcgttt taccttgtga aataagcatc tgtacagcct
      181 ctgaagaagc ttttacaggt cttcccatat gtaccccttc agctttagca cgttcaatac
      241 cagcctttct acgctctgct atcaagcttc tttcaagctg tgccaacgtt gacataatgc
      301 ctaacattgc ttcacccata gcgcttgata agtcaaatcc ttctctaagg ctgataactg
      361 tcacaccttt agcctgtagc gctttaaccg ttgataacac atcaattgtg tcacgtccaa
      421 gacggtctac agctaccaca actacggtgt caccttctcg tgcataagct aacaaactgc
      481 tgaaaccgtt tcttaaagaa gctttgacag aaccactgac agcttcatca gcgaaccatt
      541 tagcaatgtt gtaccctgcc tcttcaattt gcatttgctg gttagtgatg gattggtcat
      601 ctgtactcac cctgcaatat ccaagtatcg ccataatgtt ccccttagaa gtctcaaaag
      661 ttagtctcat aagcttagct gtctcataat ccaaaaacaa ccttttgaga ctacttgagg
      721 gtgcattcta ccgttacgtt gaaagtgtct caaaaggact tccttatgag actggttaat
      781 agagcgctat gaagattcag gagggtaact ataaccatca ctttctaact ttcacgtagg
      841 aaatcgttaa gtttcttgat agttacacca tgtgaccaac tatctatata tcgttatata
      901 gccctgtata tagcactata tgtagtgtct ggagagggac gactaacacg atacagttta
      961 ccctgaaaag agacttcaca gattctttat atatttcaat aagttatgct aaaaagagac
     1021 tataccgata ctgcatagaa tttgtgtagg attgcataga tacttggaaa aacaccgtta
     1081 aattgcatag tcattcacct gatattgtcc tgtttttaca ggtagttgat tcggatgttc
     1141 taggaatcat cctcatagtt gattcggtta gttgggcttg taccaacccc taatcaacct
     1201 catagtgtat ttagaccttg atgattttag acagccgccc tgttcatgca gcacgtccac
     1261 acagtgaagc tattcgcgac gctcaacgaa aaaaacctaa agttccggta catgctgtac
     1321 tgacggcgac aaatccgctg attcgtttta tcggtagtga tgatatgacg caaaaccggg
     1381 aattatttca ggtctggtta cagaaattag cgcagtggca tcagaccact acgccttatc
     1441 tttttttaca tacgccagac attgcccagg ccccggaact ggtacatacc ctgtgggaag
     1501 acttacgtaa aacgcttcca gagatcggag cagttccggc tattccacag caatcttctc
     1561 ttttctgaat ttgccaccta tcatagacag gtgccatcgg ccattttaaa gggagtttgt
     1621 atggtaagcg cgctatatgc cgttttaagt gcgttgttat taatgaagtt ctcttttgat
     1681 gtcgttcgcc tgcgaatgca gtaccgcgtt gcctatggtg acggtggttt tagcgaactg
     1741 caaagcgcta ttcgcattca tggtaacgcg gtggaatata ttcccatcgc gattgtgctg
     1801 atgctgttta tggaaatgaa tggcgcagaa acctggatgg tgcatatttg cggcatcgtt
     1861 ttgcttgctg gtcgtctgat gcattattac ggttttcatc accgtctgtt tcgctggcga
     1921 cgttccggca tgagcgccac ctggtgtgcg ctgttgctga tggtgctggc gaatctttgg
     1981 tatatgccct gggagttggt tttctccctg cgttagcgca caatacgcca ctttcttttt
     2041 cccggatttt tacgttatgt ctcaccgcga cacgctattt tctgccccta tcgccagact
     2101 gggcgactgg acctttgatg aacgggtagc tgaagtcttc ccggatatga tccagcgttc
     2161 cgttcccggc tattccaata ttatttccat gattggtatg ttagccgagc gcttcgttca
     2221 acctggtacg caggtttacg atctgggttg ttctctgggc gcggcgacgc tctcggtgcg
     2281 tcgcaacatt catcatgata attgcaaaat tattgccatc gacaactccc cggcgatgat
     2341 tgaacgctgc agtcgtcata ttgacgccta taaagcccct acgccagtag acgtcattga
     2401 aggtgatatt cgcgatatcg ccattgaaaa cgcatcgatg gtggtgctga attttaccct
     2461 gcaattcctg gaaccttccg agcgccaggc attactggat aaaatttatc aaggactgaa
     2521 cccgggcggc gcgctggtgc tttcggaaaa attcagtttc gaagatgcca aagttggtga
     2581 actgttgttc aacatgcacc acgactttaa acgtgccaac ggttacagcg aactggagat
     2641 cagccagaag cgcagcatgc tggaaaacgt gatgctgacc gattccgtgg aaacccataa
     2701 agcacgcctg cataaagccg gttttgagca tagcgagctg tggttccagt gctttaactt
     2761 tggttcactg gtggcattaa aagcagagga cgctgcatga tcgactttgg taacttttat
     2821 tctctgattg ccaaaaatca tctttcacac tggctcgaaa cgctgcccgc gcagattgct
     2881 aactggcagc gcgagcagca gcacgggctg tttaagcagt ggtccaacgc ggtggaattt
     2941 ctgcctgaaa ttaaaccgta tcgtctggat ttattgcata gcgtaaccgc cgaaagcgaa
     3001 gagccactga gcaccgggca aattaaacgc attgaaacgc ttatgcggaa cctgatgcca
     3061 tggcgcaaag ggccgttctc actgtatggc gtcaacatcg ataccgaatg gcgttccgac
     3121 tggaaatggg atcgtgttct gccccatctt tctgatttaa ccggacgcac cattctggat
     3181 gtcggctgcg gcagcggtta tcacatgtgg cgcatgattg gcgcaggcgc gcacctcgcg
     3241 gtggggatcg accctacgca actgtttctg tgccagtttg aagcggtgcg taaactactg
     3301 ggtaacgatc agcgcgcaca tttgttaccg ttaggtattg aacagcttcc ggcactgaaa
     3361 gcctttgata ccgtcttttc gatgggcgtg ctttaccatc gccgttcgcc gctggagcat
     3421 ctctggcagt tgaaagatca actggtgaat gagggtgaac tggtgctgga aacgctggtt
     3481 attgatggcg atgagaacac ggtgctggta ccaggcgatc gttacgcgca aatgcgtaat
     3541 gtctatttca tcccttccgc gctggcgctg aaaaactggc tgaagaagtg tggttttgta
     3601 gatatccgca ttgtggatgt gtgcgttacc actactgaag agcagcgacg cactgaatgg
     3661 atggttaccg aatctctgtc cgatttcctc gacccacatg atccgagtaa gacagttgaa
     3721 ggttatcctg cgcctaaacg cgcggtgctg attgcgcgca agccgtaaag gtctggtatt
     3781 actgccggat gcggcgtgaa tgccttatcc ggcctacaaa cccttgctaa ttcaatctat
     3841 tgcagggact atgtaggcct gataagcata gcgcatcagg cagttttacg tttgcataac
     3901 ctcagcgccc gtttccgggc gctattcact tcttacgcct gaacagcagg cttatcaaat
     3961 gccgttaact tcggtgcgtt acccgtatat ttttcgatat acaccagcgc agagttaccg
     4021 gcacaggcat ttgccagctg cgagctgggg atatccgccg ttaacacgtt cgcactgccg
     4081 tttttacaca agccgttttc cagatctggc catgcacctt catgcacgca taccacgcct
     4141 tttttgatcc cgtcagtcac caccgcgcct gtcaaaatct gaccacgttt gttccagaca
     4201 cgcaccagat cgccattcgc aatactaaat cgagcagcat cttcggtgtg aatagtgatc
     4261 ggttcacgat cggcaaccgc atatttttta cgcagttccg catagttaag ctgactgtgt
     4321 aaacggtgtg ccggatgtgc ggtcagaagc tgcaactgct tctcgtcggc ggtacccttc
     4381 cactcatcag gcgcaagcca ggttgggtgt gccgggcaat ccttatagcc aaatttttcc
     4441 agcgttttgg agtaaatctc aattttgcca cttggcgtac ccagcgcatt tttcaccgga
     4501 tcggcgcgga aatcaccata acgaacgtac tgttcgttct tctcgctgcg gcgcatttcg
     4561 atcagtttat tttgctgcca gaaggcatta aacattggca tagtgacgcg ttgcgcacgg
     4621 gcccctttct gagcggcatc atagaaaaat ttcagccacg ccatttcatc tttaccttcg
     4681 gtatagatct cttttccgcc aggtttgagt aattccgcaa gatcggcaaa cacatcaaaa
     4741 tcgtttcgcg cttcaaattg cggagcaaca gcctgcttca tcggcacaat atgctggttg
     4801 ctgtaatcac cggtcatcgt caagtcattg cgctcaaacg atgtggtgat cggtaatacg
     4861 atatctgcgt gtttagcagc tgcggtccag tagcattcag aaacgacgat catctccggt
     4921 ttctgccatg ctttaatcag acgattagta tcctggtgat gggtaaagtt accgccaccc
     4981 gcccaccaga tcattttgat attcggatac gtttgctctt taccgttatg ctgatatttg
     5041 ccgccaggat tttccagcgc atcgacgata cgggcaacag gaatcgccct cattccgcca
     5101 tcatcagcgg cctcgctggc ctgtccggca atcgccgcag acatttccgg cagcacgccg
     5161 ccaacacgtg tcgggttgcc accattggag taatgataag agaaaccaaa accgccgccc
     5221 ggtgtaccaa tctgccctaa catcgccgcc agcgtcacca gcatccagtg tttctgttca
     5281 ccatattgct ggcgctgaat tccccagcct gccatcagca ttgtgcgatt cgccgccatc
     5341 agttcagcga gtttgactat ctgcgcttcc ggtacgccag taatttccgc cgcccaggcc
     5401 gcgctcttcg gcgtattatc gctcttacct ctcagatact cttcaaactg cggatacccg
     5461 gtagtgtatt tttcaaggaa cactttatcg tgtttgcctt gtgtcatcag ggtatgcgca
     5521 atccctaaca tcagtgccac gtcggtgccc atattcggcg cgatccaggt ggcattatcg
     5581 ccaaagaagt cgatggtttc ggagcggata ggatcaatgg caatcactgg tttgccagat
     5641 tttttcagct gatggaagta ttccagccct tgctcatcgg tactgctcca ggcaattttt
     5701 aaggtattca gcgggttcat cccccacagc accacaacct ggctgttttc cagaatcagc
     5761 ggccaggagg tctgctgttc atacacctct acagaaccga ccacatgcgg catgatcacc
     5821 tgtgccgccc cggttgaata atcgccgcta tgcccggaat aaccgcccgc caggttcata
     5881 taacgttgca gtaaggtttg cgctttatgc aacacgccag aagagcgcca gccgtatgat
     5941 ccggcaaaaa tagccgatgg tccgttagct ttgcgaatgc gatcgtgttg ctcatgaatc
     6001 agttttaatg cttgttccca gctcacctgt acataggtat cttcgccacg acctttcgtc
     6061 ggttgcagtg gattatcgag atagcttttt ctcaccatcg gatgctgaat acgcgtcgtg
     6121 gtgtgtacct gatccgccgc cgtagactgt aaggaattcg gtatggtttt cgccagcgcg
     6181 cctgttgaag aaacaatctt gccgtccttc acttctacgt tcatcgctcc ccaacgttcc
     6241 gcggtgagga ttttaccgtc
//
LOCUS       DADMKF010000198         6252 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.202, whole genome shotgun sequence.
ACCESSION   DADMKF010000198 DADMKF010000000
VERSION     DADMKF010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6252
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.202"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            121..951
                     /locus_tag="JNC24_003716"
     CDS             121..951
                     /locus_tag="JNC24_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418745.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfZ/YjiC family protein"
                     /protein_id="HAY9160367.1"
                     /translation="MSMPLSNALQSQIITDNHFLHHPIVDSEFTHKLKYARMDLENIY
                     LPPLTRGNNHNYDGKSAVEIRKLDISKESWPFNYVTGACRESDGITTTGRMLYRNLKI
                     TSALDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGCNVPGYA
                     GTTIGYISTLPASQAKRWTNEQPRIDIYIDQIMTVTGVANSSGFALAALLNANIELGN
                     DPIIGIEAYPGTAEIHAKMGYKVIPGDENAPLKRMTLQPSSLPELFELKNGEWNYIGK
                     "
     gene            complement(1092..1865)
                     /gene="uxuR"
                     /locus_tag="JNC24_003717"
     CDS             complement(1092..1865)
                     /gene="uxuR"
                     /locus_tag="JNC24_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HAY9160368.1"
                     /translation="MKSATSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWRHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(2080..3540)
                     /locus_tag="JNC24_003718"
     CDS             complement(2080..3540)
                     /locus_tag="JNC24_003718"
                     /EC_number="1.1.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HAY9160369.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRVLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMRYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(3621..4805)
                     /gene="uxuA"
                     /locus_tag="JNC24_003719"
     CDS             complement(3621..4805)
                     /gene="uxuA"
                     /locus_tag="JNC24_003719"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAY9160370.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLVQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            5145..>6252
                     /gene="gntP"
                     /locus_tag="JNC24_003720"
     CDS             5145..>6252
                     /gene="gntP"
                     /locus_tag="JNC24_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HAY9160371.1"
                     /translation="MHVLNILWVVFGIGLMLVLNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIIS"
BASE COUNT         1480 a         1618 c         1586 g         1568 t
ORIGIN      
        1 tgtagagttg atatcactac aacagaaaca tgcatgtcat ttgtttactt ctctttttac
       61 tacagaaaaa tttatctatt tttctaaaca tcagtgaaat actcataaaa ggtaactatt
      121 atgtctatgc cattaagcaa tgcccttcag agtcaaatta ttacagataa tcacttcctt
      181 catcacccaa tagtcgacag tgagtttaca cataaattaa aatatgcacg catggatttg
      241 gaaaacatct atcttccgcc tcttactcgt ggcaataacc ataattacga tggaaaaagc
      301 gctgtcgaaa ttagaaaact tgatattagt aaagagtcct ggccatttaa ttatgtgaca
      361 ggagcatgca gagaatccga tggtattaca acaacaggac gtatgctcta tcggaacttg
      421 aaaatcacct cagctttaga cgaaatatat ggtggcatat gtaaaaaggc gcatgctgcc
      481 acagagttag cagaaggttt acggctaaat ttatttatga agagcccttt cgatcctgtt
      541 gaggactata cagttcatga aataacatta ggccctggtt gcaatgttcc tggttatgcc
      601 ggaaccacga taggatatat ttcaaccctt ccagcttctc aggcaaaaag atggaccaat
      661 gagcagccaa ggattgatat ctatattgat cagataatga cggtaacagg ggtagcaaat
      721 tcttccggat tcgcccttgc cgcattactt aatgctaata ttgagttggg taatgaccca
      781 ataattggta tagaagccta tcccggtacc gcggagatcc atgcaaaaat gggatataaa
      841 gttatcccgg gtgatgaaaa tgcaccactc aaacgtatga ctctgcaacc gtcatctctt
      901 ccagaactgt ttgaattaaa aaatggtgag tggaattata ttggtaaata actaccagga
      961 agaatgagta ctaacattca ggttatacag atagctaaac aataccctgc aatttacaaa
     1021 gtaatactca ttgcgacttc ttaatttaag aaagcgcaag gaacatttac ccttgcgctt
     1081 attataataa gtcaggcgtc aactttatcc agcggccatg aatcaaacag atagccatca
     1141 aaataaatat cgtcaacgtt cgagaattcc agcagacgtt gcttaacgtt ttccagatgc
     1201 cgccacattg ccagcttcgc cgctcgggca tcttttttga ttaacgcggc gaggatctgt
     1261 ttgtgatcgc ccaaccactc tttgcgatac aggctgtcat ccagatggct gtgcaactgg
     1321 atccacattg gattgttttc ccgccactgc caggactgac ggaacagctc caccagcatg
     1381 ctgttatgcg ttgcttcggc aatggcgaga tggaactgca tatcaccact ttcgctgctg
     1441 cccggcgcgc tggaagccag ctcacgctct tccagttgca atgcctgacg cattttgacg
     1501 atatcttcgc gggtagcctg caaagcggca aattccgcga tgttactttc cagtaactga
     1561 cgcgcctgta acagctcaaa aggaccggca tcattgcaga cgttagcatc cggactgtct
     1621 gtgttgtggc tgcctgagct atcaagaaca tagataccgg caccccggcg cacttccacc
     1681 agccctttga tctccagcat gatcagcgct tcacgtacca ccgtcctcgt gacatcaagc
     1741 atttctgcaa tttcacgctc cggcggcagc cgttcgccag gattgtatgg cgtctttatg
     1801 atcagatcgc ggatcatcgc cccgacttcc tggtaaggtc tttgcgcaga ggtggcagat
     1861 ttcatggtgt tgtgtccgtt aggtgcggtt aatcaaattt ttgccggatg cggcgtgaac
     1921 gccttatccg gcctacaacg ggcaatttta cggcacccgt taatctcaaa ctataccatt
     1981 agcactggaa gttggcacgt acaggtgggc aaaaaaacga aaactggtca accaaatatc
     2041 agtttgattg accagtaagt ctgtagggta atcagttagt tacagcgcag ccacacactc
     2101 gcgcgcaccg cgttcgcaca gctgctgata tgccgccgtc accgcgccaa caaagtcggc
     2161 attctgcggc agatcatcgg caaaaatgcc gctcaggccc agcagcgctt tcacgcggtc
     2221 tgcgccctga tactgcgcgt taatcttctg gaactcggcc agcatcgggt caaccacgtc
     2281 aatggcatta ccctgttcat ccacgccctg ggtgtagcgc atccagccag ccacgcccag
     2341 tgccaggtga cgccagctgc cgccgttttg caggtgcaga cgcaccggat ccagaagacg
     2401 ctgcggtaac ttctggctgc cgtccattgc aatctgccag gtacggtgac gcagagacgg
     2461 gttgctgaaa cgctcgatca gcagcgtcgc ataggcgttc aggtctgtac cttccggcat
     2521 cgacagcgtt ggcgcttgtt cctgcatcat cagggcaaag gccgctttgc gataatccgg
     2581 attagtcatg gtgtcggcaa tggtttcata gccgccgagg taaccgaggt acgccaggaa
     2641 agagtggctg ccgttcagca tacgcagttt catcatttcg aacggcacaa cgtctgcaac
     2701 gaactgtgcg cccactttat cccagtccgg acgaccatta acgaagttat cttcaatcac
     2761 ccactgacgg aacggttcgc aggcaatggc gcacgggtcg taaacaccca gctggtcagc
     2821 aatttcctgt aaggtttctg gcgtcgccgc cggaacgatg cggtcaacca tggtgcacgg
     2881 gaaggtgacg ttctcttcaa tccatgccgc cagctgcgga tcacgtgcct gagccagccc
     2941 cagaaccgcg accttcgcca catgaccgtt ttcacgcacg ttgtcgcagg acatcaccgt
     3001 aaacgctttc agcccttttt cacgacgcag acgcaaggct tcgacgatgt aaccaatcgc
     3061 ggacttcggc gcagtcgggt tttccagatc gtgcttgatc agcgggttat tgagatccag
     3121 ctgaccgctt gccgcatcgg cgcaataacc tttctctgtg accgtcaggg agacaatcgc
     3181 cgtctgcgga cgcgccatcg cgttgagaat accttcacaa ccgtcgattt ccggatgcag
     3241 ggcttctttc atcgaaccga taattttcag ctcggtgctt tctgcgcctt tttccgccac
     3301 ggtgtacagc agttgctgtt ttttcaggtt ttcaatcagc acacggtcgt tgcctggcat
     3361 caggttaact tcgcagatac cccagtcgct gtcggtactt tccagcagat gatgggtgta
     3421 cagcgcctgg tgcgcgcggt gaaacgcccc gcaaccgaga tgcacaatgc gtgattccag
     3481 acgagaatga tcccatgacg ggcgggcaac cggcagattg ctgtcaacaa tagtagtcat
     3541 cgtaaactcc agaatccgtc ttcagagagt gacggcaagg aagaaggaac cggcacggcg
     3601 cgcagccatg ccggtgggta tcaacggcta aagaaagcgc gctggatcgc cagttcgaca
     3661 ccgcgaactt cagccaggcc tttcagacga ccaattgcgg agtaacctgg gttggttttc
     3721 ttcttcaggt cgtccagcat ctgatgaccg tggtccggac gcatcgggat caggtcttct
     3781 ttgccttccg ctttacgacg gtgttcttct tcaacaatcg ctttcaccac ttcgtacata
     3841 tcaacgtcac cgttcaggtg cgccgcttcg tggaaggttt tcgggttatc ttcacgcatg
     3901 gtggagcgca gatgggtgaa gtaaatacgc ggaccgaact gcttgatcat atcaaccaga
     3961 tcgttgtcag cacgcacgcc gtaggaacct gtgcacatgg tgaaaccgtt cgccatgctg
     4021 tttacggtat caaccatcca ctgcatatct tcaatggtgg aaacaatgcg cggcaggccg
     4081 aggatcgggc gcggcggatc gtccgggtga acagccatac ggacgccaac ttcttcggca
     4141 accggaataa tcgctttcag gaagacggca aagttttcgc gcagtttggc tttgtcgata
     4201 tctttataca gctccaggtg cttacggaac tggtcgaggg tatacccttc ttccgcgccc
     4261 ggcagaccgg caatgatgtt acgggtcaga cgcgctttgt cttcgtcgct catagtggcg
     4321 aagcgttcag cggcctgagc aatttcttct tcggtgtaat ccgcttccgc gcctggacgc
     4381 ttcaggatat gcatttcgaa tgcagcgaat tcgatctggt cgaagcgcag agctttggag
     4441 ccgtctggca gcacgtattc gaggtcagta cgggtccagt cgagcaccgg catgaagttg
     4501 tagcacacgg tgcgaatgcc gcactgcacc aggttgcgca gagtctgctg atagttagcg
     4561 atccactgtt catagttgcc agtgtgggtt ttgatatctt cgtgaattgg cacgctttct
     4621 acgacagacc ataccaggcc tgcgtcttca acgatcgcct tgcgtttgag gatctcttct
     4681 acggaccata cttcgccgtt cgggatatgg tgcagcgcgg taaccacgcc agttgcgccc
     4741 gcctgacgga catcagctag agaaaccgga tcgtttgggc cgtaccagcg ccaggtctgt
     4801 tccatgattt ttcctctttc acatagtggg ttccacagca aagagacgtg gagccccgga
     4861 gttgtttagg tatccggatg aacaaccaga ccgcagccgt ggcgttgcat cggatgagat
     4921 ggcgtataag ttctaccttt tggtaagaca tgtcaacccg aaatctgaaa ttggttaacc
     4981 acatcgcaag aatttcactc tcacggttgt tgctgcaaat tgcagcaaag ggtaatcgat
     5041 ggttattgat cagaggatgt gacattcatc gcaacaatgg ttgaccaatt tacataacat
     5101 gtcgcccaaa ataacaacgg ttcaaccagg taaggtaaaa cgttatgcat gtgcttaaca
     5161 ttctctgggt ggtattcggc attggcctga tgctggtact gaatttgaag ttcaaaatca
     5221 attcaatggt ggctttgcta gtggcggcgc tgtccgtcgg gatgctggcg ggcatggatt
     5281 tgatgtcgct gctgcacaca atgaaagcgg gcttcggcaa cacgctgggg gaactggcta
     5341 tcatcgtggt gttcggtgcg gtcatcggta aattgatggt cgactccggc gcggcgcacc
     5401 agatagcgca tacgctgctg gcgcgtctcg gtctgcgcta tgtacagctg tcggtaatta
     5461 tcatcggcct gattttcggt ctggcgatgt tctatgaagt ggcctttatc atgttagcgc
     5521 cgctggttat tgtcattgcc gccgaagcta aaatcccgtt tctgaaactg gcgatcccgg
     5581 cagtagcagc tgccactacc gcacattcac tgttcccacc gcagccgggt ccggtggcgc
     5641 tggtgaatgc ttatggcgcg gatatgggga tggtttatat ctatggcgta ctggtgacga
     5701 tcccaagtgt catctgcgca ggtctgatcc tgccgaagtt cctcggcaat cttgagcgcc
     5761 caacgccatc attcctgaaa gcagatcaac cggtagatat gaataatctg ccctctttcg
     5821 gcgtttcgat tctggtgccg ctgatcccgg cgatcattat gatctctacc accatcgcca
     5881 atatctggct ggtaaaagat acccctgcct gggaagtggt taactttatc ggttcctcgc
     5941 cgattgcaat gtttattgcg atggtggttg cattcgtact cttcggcacc gcgcgcgggc
     6001 acgatatgca gtgggtgatg aacgcttttg aaagcgcggt gaagagcatt gcaatggtga
     6061 ttctgatcat cggtgcgggt ggcgtgctga agcagaccat catcgacacc ggcattggtg
     6121 acaccatcgg catgttaatg tcccacggca atatctcgcc ctacatcatg gcatggctga
     6181 tcaccgtact gattcgtctg gcgacgggtc agggtgtggt ttcagcgatg accgccgccg
     6241 ggattatcag tg
//
LOCUS       DADMKF010000199         6130 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.203, whole genome shotgun sequence.
ACCESSION   DADMKF010000199 DADMKF010000000
VERSION     DADMKF010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6130
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.203"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            <1..225
                     /locus_tag="JNC24_003721"
     CDS             <1..225
                     /locus_tag="JNC24_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY9160372.1"
                     /translation="DSYDNAMAEIIKGLYKAEVIHRKSWKNRTEVELATLTWVDWYNN
                     RRLLERLGHTPPAEAEKAYYASIGNDDLAA"
     gene            271..621
                     /gene="traA"
                     /locus_tag="JNC24_003722"
                     /pseudo
     CDS             271..621
                     /gene="traA"
                     /locus_tag="JNC24_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673167.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system pilin TraA"
     gene            636..947
                     /gene="traL"
                     /locus_tag="JNC24_003723"
     CDS             636..947
                     /gene="traL"
                     /locus_tag="JNC24_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraL"
                     /protein_id="HAY9160373.1"
                     /translation="MLGDENKLKKYRFPETLTNQSRWFGLPLDELIPAAICVGWGITT
                     SKYLFGIGAAVLVYFGIKKLKKGRGSSWLRDLIYWYMPTALLRGIFHNVPDSCFRQWI
                     K"
     gene            969..1151
                     /locus_tag="JNC24_003724"
                     /pseudo
     CDS             969..1151
                     /locus_tag="JNC24_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490565.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1132..2403
                     /locus_tag="JNC24_003725"
     CDS             1132..2403
                     /locus_tag="JNC24_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001500202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAY9160374.1"
                     /translation="MCPLPCHTYRNQLAFIDIFFRGMNNTNYNMAVCGTSGAGKTGLI
                     QPLIRSVLDSGGFAVVFDMGDGYKSLCENMGGVYLDGETLRFNPFANITDIDQSAERV
                     RDQLSVMASPNGNLDEVHEGLLLQAVRASWLAKENRARIDDVVDFLKNASDSEQYAGS
                     PTIRSRLDEMIVLLDQYTANGTYGQYFNSDEPSLRDDAKMVVLELGGLEDRPSLLVAV
                     MFSLIIYIENRMYRTPRNLKKLNVIDEGWRLLDFKNHKVGEFIEKGYRTARRHTGAYI
                     TITQNIVDFDSDKASSAARAAWGNSSYKIILKQSAKEFAKYNQLYPDQFQPLQRDMIG
                     KFGAAKDQWFSSFLLQVENHSSWHRLFVDPLSRAMYSSDGPDFEFVQQKRKEGLSIHE
                     AVWQLAWKKSGPEMASLEAWLEEHEKYRSVA"
     gene            2400..2786
                     /gene="trbI"
                     /locus_tag="JNC24_003726"
     CDS             2400..2786
                     /gene="trbI"
                     /locus_tag="JNC24_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TrbI"
                     /protein_id="HAY9160375.1"
                     /translation="MTTTQKTTDVTAPRRSHWWWTVPGCLAMVLLNAAVSYGIVRLNA
                     PMTAAFNMKQTVDAFFDSASQKQLSEAQSKALSARFNTALEASLQAWQQKHHAVILVS
                     PAVVQGAPDITREIQQDIARRMRAEP"
     gene            2783..4087
                     /locus_tag="JNC24_003727"
     CDS             2783..4087
                     /locus_tag="JNC24_003727"
                     /inference="COORDINATES: protein motif:HMM:NF023893.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005143240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase"
                     /protein_id="HAY9160376.1"
                     /translation="MRCRGLIALLIWGQSVAAADLGTWGDLWPVKEPDMLTVIMQRLT
                     ALEQSGEMGRKMDAFKERVIRNSLRPPAVPGIGRTEKYSSRLFDPSVRLAADIRDNEG
                     RVAGFESAYVALSRMKQHVQVYTDNRQGWTDAINNAVQKGTAHDVFEPKPDREVMNAE
                     RLFSTARELRDVAAGRAVLRQAGLAGGDSPARFIAPGRKYPQPYVALPAFDRNGKSAG
                     IWLNPLTTDDGNGLRGFSGEGRVKGSGDAQFVALQGSRNGESLLADNMQDGVRIARDN
                     PDSGVVVSIAGEGRPWNPGAITDGRVWGDIPDNSVQPGAGNGEPVTAEVLAQRQAEEA
                     IRRETERRADEIVRKMAENKPDLPDGKTELAVREIAGQERDRAAITEREAALPESVLR
                     ESQREREAVREVARENLLQERLQQMERDMVRDLQKEKTPGGD"
     gene            4107..4854
                     /gene="traX"
                     /locus_tag="JNC24_003728"
                     /pseudo
     CDS             4107..4854
                     /gene="traX"
                     /locus_tag="JNC24_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858383.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     acetylase TraX"
     gene            4909..5469
                     /locus_tag="JNC24_003729"
     CDS             4909..5469
                     /locus_tag="JNC24_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HAY9160377.1"
                     /translation="MTEQKRPVLTLKRKTEGETPVRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPTLDEAVNTLKPWWPGLFDGDTPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEA
                     YAAARLDKIRRQNRIKAELQAVLDEK"
     gene            5599..5811
                     /locus_tag="JNC24_003730"
     CDS             5599..5811
                     /locus_tag="JNC24_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HAY9160378.1"
                     /translation="MNGFRNSSRNGQVWRYQRAGGRAVILEVSGRWMEAAEAWRRAAC
                     VAPRTDWQQFARKRAEHCHRRCRGRV"
BASE COUNT         1462 a         1484 c         1865 g         1319 t
ORIGIN      
        1 gactcgtatg acaacgcgat ggcagagatc atcaagggcc tttacaaagc ggaggtaata
       61 caccgtaaga gctggaaaaa ccggacagaa gtggagctgg ccacactcac gtgggtggac
      121 tggtataaca atcgacgatt gctggaaagg ctgggccata ctcctccggc agaagcagaa
      181 aaagcttatt atgcttccat cggaaacgat gatctggcag cctgagttca cagataaaac
      241 actctccagg aaacccgggg cggttcagcg ttaagtgttc agggtacttc tgcgcccgtc
      301 aaaaagaaat cgtttttttc taaattcact cgtctgaata tgcttcgcct ggttcgtgca
      361 gtgatcccgg ttgctgttct gatgatgttg ttccctgagt tagctatggc tgctggtaaa
      421 ggcacggacc tgatggctaa aggtcaggaa acggttaagg cgaccttcgg taaggactcc
      481 agtatcgtta aatgggttgt tctggctgaa gttctggtcg gtgcagtcat gtacatgatg
      541 accaaaaacg tcaagttcct ggtcggcttt gccatactct ctgtatttat tgcagtaggt
      601 atgtctgttg ccggattctg acaggaaata aaacgatgtt gggagacgag aataaactta
      661 agaaatatcg tttcccggaa acactgacca accagagccg ctggtttggc ctgccactgg
      721 atgaactgat ccccgcagca atctgtgttg gctggggtat cacaacatcg aaatatctgt
      781 tcggtattgg tgcagcggtt ctggtttatt tcgggattaa aaaactgaaa aaagggcggg
      841 gcagttcctg gttacgtgac ctgatttact ggtatatgcc aacagccctg ctgcgcggta
      901 tttttcataa tgttcccgat tcgtgtttcc ggcagtggat taaatagaac tgataccagg
      961 attgttatat ggaacacggt gcccgtttaa gtaccagtcg tgtaatggcc atcgccttta
     1021 tatttatgtc agtgcttatt gttctcagcc tctctgttaa cctcattcag ggggtgaata
     1081 actaccgtct tcagaatgag caacgcactg ccgtgacccc aatgggattt aatgtgccct
     1141 ttgccgtgtc acacctaccg taaccagctg gcgtttatcg atattttctt ccggggaatg
     1201 aataacacca actacaacat ggcggtctgt ggcacctccg gggccggtaa aaccgggctg
     1261 atacagccac ttatccgcag cgtgctggac tccgggggct ttgccgtggt gttcgacatg
     1321 ggggatggct acaagtccct gtgtgagaac atgggtggcg tatatctgga cggtgaaacc
     1381 ctgcgtttta acccgtttgc gaacatcacc gatatcgacc agtcagcgga gcgtgtccgt
     1441 gaccagttgt cagtgatggc cagccccaac ggtaacctgg atgaagtgca tgaaggtctg
     1501 ctgctgcagg cggtcagggc ctcctggctg gccaaagaga acagggcacg tattgatgac
     1561 gtggtggatt tcctgaaaaa cgccagtgac agcgagcagt atgccgggtc accgactatc
     1621 cgcagccgtc tggacgaaat gattgtgctg cttgaccagt acactgccaa cggcacttac
     1681 ggccagtatt ttaactctga tgagccgtcc ctgcgggatg acgcaaaaat ggtggtgctg
     1741 gagctgggcg gactggaaga ccgtccgtca ctgctggttg cggtgatgtt ctccctgatt
     1801 atctacatcg agaacaggat gtaccgcacg ccgcgtaacc tcaagaaact gaacgtcatt
     1861 gatgaaggct ggcgtctgct ggacttcaaa aatcacaagg tcggtgagtt tattgagaaa
     1921 ggttaccgta ccgcccgtcg tcataccggt gcttatatca ccatcacaca gaacatcgtc
     1981 gactttgact ctgacaaggc ctccagtgcg gcccgtgcgg catggggtaa ctcctcctac
     2041 aaaattatcc tcaaacagag tgcgaaggag ttcgcaaaat acaaccagct gtatccggac
     2101 cagttccagc cactgcagcg cgacatgatt ggtaagtttg gtgcggccaa agaccagtgg
     2161 ttcagttcct tcctgctgca ggtggaaaac cattcctcct ggcaccgcct gtttgtggac
     2221 ccgttaagcc gcgccatgta cagctctgac ggcccggatt ttgagtttgt gcagcagaaa
     2281 cgtaaagagg ggctgagtat tcatgaggcg gtgtggcagc tggcgtggaa gaagtcaggg
     2341 ccggagatgg cttcgctgga agcctggctg gaagaacatg agaaatacag gagtgttgca
     2401 tgaccacaac gcaaaaaacg actgatgtta cagccccccg acgcagccac tggtggtgga
     2461 cggtgccggg atgcctggca atggtgttac tgaacgcagc cgtcagttac ggcattgtca
     2521 gactgaacgc accgatgact gccgccttca acatgaaaca gactgtggat gcattttttg
     2581 acagcgccag tcagaaacaa ctgtcagaag cacagtcaaa agccctttca gcgcgtttta
     2641 acacggcact ggaagccagc ctgcaggcgt ggcagcagaa acaccacgcg gtcattctgg
     2701 tgtcgcctgc cgtggtacag ggggctccgg atatcacccg ggaaatccag caggatattg
     2761 cccggcgaat gagagcggaa ccatgagatg ccgggggctg attgccctgc tgatatgggg
     2821 gcagagtgtg gccgccgccg atcttggcac ctggggtgat ctgtggccgg taaaggagcc
     2881 ggacatgctg acggtgatca tgcagcgcct gacggcactg gagcagtccg gtgagatggg
     2941 ccggaaaatg gatgccttta aggagcgggt gatccgcaac agtctgcggc cccctgccgt
     3001 gcccggtatc ggacgcacgg agaaatacag cagccggttg tttgacccgt ccgtcagact
     3061 ggctgcggat atccgggata acgaagggcg ggtggccggc tttgagtcag cctacgtggc
     3121 cctgtcgcgt atgaagcagc atgtgcaggt gtacaccgat aaccgtcagg gctggacgga
     3181 tgccatcaac aatgccgtac agaaaggaac tgcccacgat gtgtttgagc cgaaaccgga
     3241 ccgggaggtc atgaatgcag agcggctgtt cagtacggcg cgggagctgc gggacgtggc
     3301 ggcaggccgt gccgttctcc gtcaggcggg gctggccggg ggagacagtc ctgcacggtt
     3361 tattgctccg ggacgtaaat atccgcaacc gtatgtggca ctgccggcgt ttgaccgtaa
     3421 cggcaagtcc gccggtatct ggctgaaccc gctgaccacg gatgacggaa acgggctgcg
     3481 gggattcagc ggtgaaggtc gggtaaaagg cagcggggat gcgcagttcg tggccctgca
     3541 gggcagccgt aacggagaaa gcctgctggc tgataatatg caggatggcg tccggattgc
     3601 ccgtgataat cctgacagtg gtgtggtggt aagcattgcc ggtgaaggcc gtccgtggaa
     3661 tcccggtgcc atcaccgatg gccgcgtgtg gggggatatc ccggacaaca gcgtccagcc
     3721 gggagccgga aatggcgaac cggtcacggc agaggtactg gcacagcggc aggctgaaga
     3781 ggccatccgc cgtgaaacgg aacgtcgcgc agatgaaatt gtccgtaaaa tggcagagaa
     3841 caaacctgac ctgccggacg gcaaaacaga gctggctgtc agggaaattg ccgggcagga
     3901 gcgtgaccgg gctgccataa ctgaacggga agccgcgctg ccggagagtg tgctgcgtga
     3961 atcacaacgg gagcgggaag cggtccggga ggttgcccgg gaaaatctgc tgcaggagcg
     4021 tctgcagcag atggagcggg atatggttcg tgacctgcag aaagagaaaa ccccgggcgg
     4081 agactgatac aggaagacaa acactgatga caacggataa cacgaacacg acacgtaacg
     4141 attcactggc tgcacggacc gatacctggt tgcagtcatt tctggtctgg tcacccggac
     4201 agcgggatat catcaaaacg gtggcactgg tgctgatggt actggaccac atcaatctga
     4261 tattccagct gaagcaggaa tggatgtttc tggccgggcg aggagccttt ccgctttttg
     4321 ccctggtgtg ggggctgaat ctgtcccgtc atgcgcatat ccggcaaccg gccattaacc
     4381 ggctgtgggg atggggaatt attgcccagt tcgcatatta tctggcaggc tttccctggt
     4441 atgaggggaa tatcctgttt gcctttgcag tggcggcaca ggtgctgacg tggtgtgaaa
     4501 cgcgcagtgg gtggcgtaca gcagcggcca ttcttctgat ggccctgttg ggggcctttg
     4561 tccggcacca gttacggcat tgccgggctg ctgatgctgg cagtcagcca ccgactgtac
     4621 cgggcggaag acagagcgga acgtctggcg ctggtcgcct gcctgctggc tgttattccg
     4681 gcgcttaacc ttgccaccag tgatgcagcg gcggtggccg gtcttgtgat gacggtgctg
     4741 accgttggtc tggtgtcgtg tgcaggaaaa tcattacccc gtttctggcc cggggatttt
     4801 ttcccggtat tctacgcctg tcatctggct gtgctgggcg ttctggcgct gtgacgggtg
     4861 tggtatcttt gaccgtaaca ggatgattcg tcagaggcag aacacagcat gacagagcag
     4921 aagcgaccgg tactgacact gaagcggaaa acggaagggg agacgcctgt ccgcagccgg
     4981 aaaaccatca tcaatgtcac cacgccacca aagtggaagg tgaaaaagca gaagctggcc
     5041 gagaaagcag cccgggaagc agagctggcg gcaaaaaaag cgcaggccag acaggcactg
     5101 tccatttatc tgaacctgcc cacgctggat gaggccgtga ataccctgaa accctggtgg
     5161 ccgggattat ttgacggtga caccccccgg cttctggcct gcggtatccg ggacgtgtta
     5221 ctggaagacg tggcgcagcg gaatatcccg ctctcgcata aaaaactgcg cagggcgctg
     5281 aaggccatca cccgttcaga aagctatctg tgtgccatga aagccggtgc ctgccggtat
     5341 gacacggaag ggtatgtgac ggagcatatt tctcaggagg aagaagcgta tgcggcagcg
     5401 cgtctggata aaatccgccg ccagaaccgg ataaaggcag aacttcaggc cgtgcttgat
     5461 gagaaataaa aaaaagcctc catcagagag gaggcaggga aataaggctt aaaaggaaat
     5521 aacttccgct tccgaaagga agccagtcca ctgaattatt tatcacaata atactgacaa
     5581 ctaaaggggg acaagaaaat gaatggattc agaaacagtt cacggaacgg ccaggtctgg
     5641 cgttaccagc gtgccggcgg gcgggccgtt attctggaag tcagtggacg ctggatggaa
     5701 gcggcagaag catggcgacg ggctgcctgt gttgcgccca ggacagactg gcagcagttt
     5761 gcccgaaaaa gggctgaaca ctgccaccgg cgctgccggg gaagggtgta acgaaaaaag
     5821 cagccttcat tactccagga gtcagaaggc tgaaaggagt ttatgagatt tgcttttttt
     5881 aatatccggt cgggcagtat gaccggacag ttctgagtag cacagagttt tttccgtatt
     5941 aagtcgtgat tttatatttt gtgattaatt tcacaaaata aggtgttatt cagtgtgtgc
     6001 tgcaatattc aggatgcctg aaatacagat gctatttatc tgatatggag aataacatga
     6061 ggaaatagta aggcagtatg actttccgtg agacagatgc tggccaacgt gggatattta
     6121 acgcgggtta
//
LOCUS       DADMKF010000200         6123 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.204, whole genome shotgun sequence.
ACCESSION   DADMKF010000200 DADMKF010000000
VERSION     DADMKF010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6123
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.204"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            <1..358
                     /locus_tag="JNC24_003731"
     CDS             <1..358
                     /locus_tag="JNC24_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS200/IS605 family element transposase accessory
                     protein TnpB"
                     /protein_id="HAY9160379.1"
                     /translation="PGRNVRAKSGLNRSILDQGWYEMRRQLEYKQLWRGGQVLAVPPA
                     YTSQRCACCGHTAKENRLSQSKFRCQVCGYTVNADVNGARNILAAGHAVLACGEMVQS
                     GRPLKQEPTEMIQATA"
     gene            complement(398..1069)
                     /locus_tag="JNC24_003732"
     CDS             complement(398..1069)
                     /locus_tag="JNC24_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HAY9160380.1"
                     /translation="MCGRFAQSQTREDYLALLAEDIERDIPYDPEPIGRYNVAPGTKV
                     LLLSERDEHLHLDPVFWGYAPGWWDKPPLINARVETAATSRMFKPLWQHGRAICFADG
                     WFEWKKEGDKKQPFFIYRADGQPIFMAAIGSTPFERGDEAEGFLIVTAAADQGLVDIH
                     DRRPLVLSPEAAQEWMRQEIGGKEASEIATNGCVPANQFTWHPVSRAVGNVKNQGAEL
                     IQPVC"
     gene            complement(1176..1409)
                     /gene="yedF"
                     /locus_tag="JNC24_003733"
     CDS             complement(1176..1409)
                     /gene="yedF"
                     /locus_tag="JNC24_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HAY9160381.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            complement(1406..2611)
                     /gene="yedE"
                     /locus_tag="JNC24_003734"
     CDS             complement(1406..2611)
                     /gene="yedE"
                     /locus_tag="JNC24_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HAY9160382.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFTALMLIIGWEKRFFRRA
                     APQTAKEIA"
     gene            2798..3211
                     /locus_tag="JNC24_003735"
     CDS             2798..3211
                     /locus_tag="JNC24_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310694.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAY9160383.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEEIGSLIVTKEGDTLDCRQWQRVIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            complement(3245..4732)
                     /gene="amyA"
                     /locus_tag="JNC24_003736"
     CDS             complement(3245..4732)
                     /gene="amyA"
                     /locus_tag="JNC24_003736"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HAY9160384.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIENPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(4810..5175)
                     /gene="fliT"
                     /locus_tag="JNC24_003737"
     CDS             complement(4810..5175)
                     /gene="fliT"
                     /locus_tag="JNC24_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HAY9160385.1"
                     /translation="MNHAPHLYFAWQQLVDKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVAPSTTMQEQLRPMLRLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            complement(5175..5585)
                     /gene="fliS"
                     /locus_tag="JNC24_003738"
     CDS             complement(5175..5585)
                     /gene="fliS"
                     /locus_tag="JNC24_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HAY9160386.1"
                     /translation="MYTASGSKAYAQIGVESAVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSLIQDPV"
     gene            complement(5600..>6123)
                     /gene="fliD"
                     /locus_tag="JNC24_003739"
     CDS             complement(5600..>6123)
                     /gene="fliD"
                     /locus_tag="JNC24_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HAY9160387.1"
                     /translation="YKEVDPGAEAQDKNNGALLGDSVVRTIQSGIRAQFANGASDSAF
                     KTLNEIGIKQDGTTGKLKIDDDKLKKVLNENTASVRELLVGDGKETGITTKIATEVKG
                     YLADDGIIDSAQDSINATLKKLTKQYLSVSASIDDTVARYTAQFTQLDTMMSKLNNTS
                     TYLSQQFTAMSNS"
BASE COUNT         1498 a         1608 c         1527 g         1490 t
ORIGIN      
        1 gccggggcgc aatgtccggg caaaatcagg tttaaaccgt tcgatactgg atcagggctg
       61 gtatgaaatg cgccgccagc ttgagtacaa gcagctctgg cgtggcggtc aggtgcttgc
      121 tgttccgcca gcgtacacaa gccagcgttg cgcgtgctgt ggtcatacag cgaaagaaaa
      181 ccgcctgtca caaagtaaat tcagatgcca ggtatgtgga tatacagtga acgccgatgt
      241 aaatggcgct cgtaacattt tagcggcggg gcacgccgtt cttgcctgtg gagagatggt
      301 gcagtcaggc cgcccgttga agcaggaacc caccgaaatg attcaggcga cagcctgaac
      361 gtagcaggga tcctcgtcct tcagggcgtg gaggatgtca acaaacaggt tgaattaact
      421 ccgcgccctg gtttttgaca ttacctaccg cgcgcgatac cgggtgccag gtgaactggt
      481 ttgctggcac acagccgttg gtagcaattt ctgatgcttc tttaccgcca atctcttgcc
      541 gcatccattc ctgtgcagct tctggcgaca gaaccagcgg gcggcggtca tgaatgtcta
      601 ccagaccctg gtctgctgca gcagtgacaa ttaaaaatcc ttcggcttcg tcaccacgct
      661 caaatggtgt gctacctatc gcggccataa aaataggttg tccatcagca cgatagataa
      721 aaaaaggctg ttttttgtcg ccttcttttt tccactcaaa ccagccatcg gcaaaacaga
      781 ttgcccgacc atgttgccag agcggtttaa acatgcgact ggttgcggca gtttctacgc
      841 gggcgttaat cagcggcggt ttatcccacc atccgggagc atatccccag aaaaccggat
      901 ccagatgaag gtgttcatca cgttcactga gcagaagaac tttggttccc ggcgcgacgt
      961 tgtatctgcc aatgggttcg ggatcgtagg gaatatcgcg ttcaatatct tccgcgagaa
     1021 gcgcaaggta atcttcacgc gtttgggatt gggcaaagcg tccacacata gaaacctcct
     1081 gagatcatac tgaaaagaat aggagagggg gcgggtaaaa tctatgtgtc agaggcaaca
     1141 atgttcccgc tgtgggccac agcgggaatg agggattact tctgaattaa ataacgaatg
     1201 gtcggcccgt cttgctgaat atccagcacc gtatagccgt gattacgcgc atccagtgga
     1261 atattattga tcgactgtgg acagtcgctc accacttcca ggatttcccc tttttttaac
     1321 tgcggcatcg cctcaagggt tgcgactgcc ggataagggc agggttcacc caccatatca
     1381 aggcggtaat caggaacgat atttttcatg cgatctcctt agcagtctgc ggtgccgcac
     1441 ggcggaagaa gcgtttttcc cagccgataa tcaacatcag cgcagtaaac aacagcaaat
     1501 atgtcaccag caggccaccc atcgggccaa aggttttcag caggttgatt ttgtcccagt
     1561 cggtggccag cgccggagcg aaatcatccc agtaatacgc cagaatcgtc gagccgatca
     1621 cattgcccag accgacccac cagtagtgca cctggccttc taccgcgcgg tacatccagc
     1681 cggtttcgca gccgcctgcc agcacgatgc caaaaccaaa cagtaaacca ccaattaccg
     1741 cgtttggtcc cgcccacatg attttgggtt caacgcctaa ctgtacgtaa ctgaagatcc
     1801 cgatggcact caccgccata ccgataatga ttgctttcgc catatgggta cgtccggtga
     1861 tccacatatc gcggaacgcc gaagtaaagc agatttgcgc acgttcaatc agtaaaccaa
     1921 agccgacgcc aaacagcatt gccagcccca gtttgggttg gttcatcgcc gtgagcagcg
     1981 cccagcccag catgccgaaa aagaccagca tcccgagacg aaaacgacgc cgcgcctgat
     2041 ccggtttttg cgtcagcggt gaggcggcag aaactttctg cattttcacg ggaatacgga
     2101 agatgggcag cagggtaaag cgcgcgccaa accatgaacc aatggcagtg gcgatggcaa
     2161 agaaccaggc atgcagcgag aactgaggaa tgccggtaaa gaacgccgcc aggttacagc
     2221 ccattgccag acgcgcgcca aaaccggcga taatgccgcc aataatggcc tgcataatgc
     2281 ggatacggct gcgcggcatg cgcagtttga cattgttggc ccacagcgct gcggcaaagc
     2341 agccgccaaa catgccgagg atcatcatcc cgtcgatgcg ggttaatggc gatccttcca
     2401 gatggataat tttaaaataa ccccactctt cagcatggac gccaaacagc tgcaggagct
     2461 gaccgcccca acgggtaaat tcacccgtga cagcccaaaa ggtgccagta atgccaaaat
     2521 agtaagtgga gagaataccc gccgcgatga ccgcagggat gggtgcccag aatttaatca
     2581 accaggcgtg tttgaattgc tgccatgaca tgaatgtagc ctctgaactc agaaggtttg
     2641 aaacaagaat cgcgaatcat acatcatgca aatttatttt cggggtaata attcacaaaa
     2701 ataatagtta gatcaaattt ctctctgaac ctggaaaggc tggcgacatc agggctttaa
     2761 tgccacaatc ctcttttctt ctcatgcagg acgaatgatg aaaaaactcg caattgcagg
     2821 tgcgctcatg ttgctggcgg gctgtgccga agtcgaaaac tataacaatg tcgtgaaaac
     2881 ccccgcgccg gactggctgg cgggctactg gcagaccaaa gggccgcaac gtgcgctggt
     2941 cagtccggaa gagataggca gcctgattgt cactaaagag ggcgataccc tggattgccg
     3001 tcagtggcag cgggtgattg ccgtgccggg caagttgaca ttgatgtctg atgatctcac
     3061 taacgttacc gtaaaacgtg agctgtacga ggttgagcgt gacggtaaca ccattgagta
     3121 cgatggtatg acgatggagc gtgtggatcg cccgacggca gagtgtgccg ccgcgctgga
     3181 taaagcgccg ttaccgacgc cactgccgta atgaaatctc cccggcttgc cgccggggat
     3241 gaatttaaat cacctcttcg ataacccaca cgctgacgct gccgccgttg caaaagaagg
     3301 tcgcttcgcc gttttcgtcg gtcactacgc tctcttgccg attccccaga aaatcacgcc
     3361 aggttttatt gccgtaattc tctcccagat gaatggtttt ttcgccatca tccccgttcg
     3421 acatgaccac cacgcagccg ggatattcgt cagtgccact gcggctaaag gcaatgcagt
     3481 tcggatggtc gaaaaataac gtctgtacac cgtgggcgaa acgctgacgg gcgagaatta
     3541 actcatcaag ctgttcgatt attggcatat ctatcggata ggtttgcccg tcaccaccga
     3601 catcttcgta atgcgcaccg tagaggtccg gatagaacac cgaaggaacg ccattttccc
     3661 gcaacaaaat tagagcatac gccagcggtt taaaccacgg ttcgaccggc gcttcgaggg
     3721 cttgcaacgg ttgggtgtcg tgattggcta cgagggttac ggcgtggaaa ggatcggctt
     3781 ccactaatgt acccgtgaaa atctgcgtca tgtcgtagtc gcgtcccatg cgcgatgctt
     3841 catggaattt catctgcagc ggcgcatcga acagcatggt tttgccttcc acctgatcaa
     3901 tatacgtttg cagtttatcg acttcatgcg accagtactc tgccacaata aacagcggct
     3961 ttggcgcaac ttcctgtacg tgttcgatcc actctttata aaaccacgcc ggaatatgtt
     4021 taaccgcatc aagacgaaaa ccgtcgcatt gtgtttgttc catcacccag cgcgcccagt
     4081 atttaatctc ttccgtcacg gcatgattgc ggaaatcgat attctcaccc atcagataat
     4141 cgaaattacc gagttcatca tcaacctgat cgttccagcc ttcgccggtg tagtcgttaa
     4201 caattttaaa tatgccatct tcatcaggat tttcgatatg gtcgataccg ctaaaacatt
     4261 tgaagtccca gataaactgc gagtattgcc cggcacgggc ggggaaggtg taacgcgtcc
     4321 agccttcgca ctcaatgatt tcttcatcaa tttgcgtacg gtcatcagca tttacacgct
     4381 gcacgcgaat agcttctttt tcatccgccc ccattttgtg gttgactacc acgtccagta
     4441 gcaccgcaat gtcattacgt ttcagagcgt caatggcggc cagcagttgt gctttatcgc
     4501 catatttagt agggatgctg cctttctgat caaactcacc taaatcaaat aaatcatagg
     4561 agtcgtagcc gaccgaatac ccgcccgatg cgcctttata ggcgggcggc aaccagacca
     4621 tattgatacc aatatcatta aaaccgtcgg cgcgctcggc cagttcaggc cagagcttac
     4681 cgccttccgg gtaataccag tgaaaacatt gtaacagcgt gggattacgc attcttcgac
     4741 tccattcaag gggaacatta gaagcgtagc cgtaatcgga ttattcgcga gccatcggct
     4801 cgttaagatt caaaagaggt tatcctgcgg agccagcaca aagccgccct gatctccata
     4861 ggcacttaac actgattttt gcaccgatga ctgaccgacc agtttcgcca gttcatccat
     4921 acgaatctgt aataactgct ttaccttgct ttcgttgtcg agaatcaggc gcagcatcgg
     4981 gcggagctgc tcctgcatcg tggtggacgg ggcaacctct tcagtcagat gtgcaatctc
     5041 ctgcaccgca ttcacatacg ccatttcgct ggcgatgagt tcgtcccatt gttcttccgt
     5101 tgccaggcgt aacatgagct ggcttttatc gacgagttgt tgccaggcga aatacaaatg
     5161 cggtgcatgg ttcatcagac tgggtcctga atcaaagaag gggagagtaa agactctttc
     5221 caggcatcgg caatattgcg cattaatgct tccacttctt cgactgcgga gacatcgttg
     5281 cgtaaattgg cttgcagcaa gcgcctgacc atatagctat aaagagcaat caagttttgg
     5341 gttagttcgt ctttgctctc ttcatcaaga ctcacccgca gcccgttctc aatgatgttg
     5401 atcgcttttg acaaagagac gcctttgcct tgctgattgt tgtcctgcat aaacaggcgc
     5461 gctctaacca gtgcgctcag cactccatca aatagcatgg tgaccagctg ttgctggctg
     5521 gcgctcatta cggcgctttc gacgccaatt tgtgcatagg cttttgagcc gctcgcggta
     5581 tacatgctga cctccgtgat caggaattac tcattgcagt aaattgttgg ctgagatagg
     5641 tactggtgtt attcagtttg ctcatcatgg tatctaattg cgtaaactgt gcagtgtaac
     5701 gcgccacggt gtcatcaatg ctggcactga cggatagata ctgtttggtc agttttttca
     5761 gtgtggcgtt aatactgtcc tgtgcgctat caataatgcc atcatcggca aggtaacctt
     5821 ttacctcggt ggcaattttg gtggtaattc ctgtttcttt accatcgcct accagcaatt
     5881 cacgtacgga tgctgtattt tcattcagga cttttttgag tttatcatca tcaattttca
     5941 gcttacccgt cgtgccgtcc tgcttaatcc cgatctcgtt taatgttttg aaggcactgt
     6001 cgctggcacc gtttgcaaat tgtgcgcgga tgccgctttg aattgtacgt acaacgctgt
     6061 ctccgagaag tgcaccatta tttttatctt gtgcttcagc acctgggtct acttctttat
     6121 att
//
LOCUS       DADMKF010000201         6108 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.205, whole genome shotgun sequence.
ACCESSION   DADMKF010000201 DADMKF010000000
VERSION     DADMKF010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6108
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.205"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(145..2412)
                     /gene="adiA"
                     /locus_tag="JNC24_003740"
     CDS             complement(145..2412)
                     /gene="adiA"
                     /locus_tag="JNC24_003740"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HAY9160388.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSTHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLSVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
     gene            complement(2611..3519)
                     /gene="melR"
                     /locus_tag="JNC24_003741"
     CDS             complement(2611..3519)
                     /gene="melR"
                     /locus_tag="JNC24_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HAY9160389.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGNITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSGWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            3802..5157
                     /gene="melA"
                     /locus_tag="JNC24_003742"
     CDS             3802..5157
                     /gene="melA"
                     /locus_tag="JNC24_003742"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAY9160390.1"
                     /translation="MMTAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALQDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICEDMTEVCPDATMLNYVNPMAMNTWAM
                     YARYLHIKQVGLCHSVQGTAEELARDLNIDPSTLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKPGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPGWLH
                     R"
     gene            5260..>6108
                     /gene="melB"
                     /locus_tag="JNC24_003743"
     CDS             5260..>6108
                     /gene="melB"
                     /locus_tag="JNC24_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HAY9160391.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEG
                     TTQIVFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
                     GVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQPSAEGSHL
                     TLKAIVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAG
                     AANLVTL"
BASE COUNT         1420 a         1451 c         1619 g         1618 t
ORIGIN      
        1 aaaaaaaagc gcggggtggg gccgcgcaaa aagtatgaca tttttgtttg cagcaatttt
       61 tatgaaaacg ttgccatact caaaaggcct aaggccaggc ggcgtaatgt tatttaaaca
      121 attacgcctt cagcggaata gtggttacgc tttcacacac ataacgtggt aaataccgtc
      181 aataatttca gtcccttcag tttcgtgttc aaatccaggg aaatggtggt cccaggattg
      241 cagcgagcgt aaataactta cttgcggact gtttttatcg ccgaagtttt caccagacag
      301 cagcatcggg attcctggcg gatatgggat aactgagttt gccgcgatgc gtcctggcag
      361 attttcaatg gataccagtt cgacattgtt gtcgacaatc gcgttgtacg cttcacgcgg
      421 ggtgatttcc gccaccgaca ggccggaata ggcttcgttc aaccgtgcgc cagggttgtt
      481 ttctttcagc caggcaaaca tggtgtcacc cagatcgtga atccccatgt tcgcgtaagt
      541 gtcaggatat tgttcaacaa gttccggcat cacctgcgcc agcggtgtgt tggcgtcata
      601 gtggcgtttg aaggagcaaa gggtgttaac cagagttccc catttcccac gggttacgcc
      661 catagagaac aggaacataa tttggaagtc agtggtgcgg gtaggcacaa tgccgtggcg
      721 accaagccag gcagtgacca gcgccgccgg aacaccggtt tcttccagtt caccatcttc
      781 acccattccc ggagcaagga tgctgacttt aatcgggtcg agcatactcc agttatccgg
      841 aatatctttg aagccgtgcc agctttcgcc cggatgcatt acccagcagt cctgaacggt
      901 ggtcagcagt ttggttggtg cgtcagcaaa gtcataggtt ttgccggttt gtgggtcggt
      961 gacgacttct ttgttccacg gtttgaagaa ccagctaccg tcagcggtga actctttata
     1021 cagccgcgcc atcgcctgac ggaaatcaac cgcttcgtcg atcacttcct gcgtcagtga
     1081 caggccgctg ttgccgtcca tcatcgacac cgccacgtcg ttggatgcgc agatggcata
     1141 cagcggggag gtggtggcat gcatcatgta ggcctggttg aagcgggaga agttaatcgc
     1201 cccacgacct tcacgtacat gaatataaga agcctgtgaa agcgcattca gcagtttgtg
     1261 ggtggagtgg gtggcgaaaa cggtaggacc gttgtgatcg ccaggttcgc cgcgcatggc
     1321 atagtgatcg gcatagatcg ggttgaaacg tgcatagccg taccaggctt cgtcaaagtg
     1381 cagacgatcg gaggtttttt ccagcagatc ctgcgcttct ttagcgttat aacacacgcc
     1441 gtcataggtg cagttggtca ccacgcagta agacggtttt tgcccggctt tgtctttagt
     1501 cagcgggctt tcactgattt tcttctgcaa ggtttcaggt tgcatttcct gcggatagat
     1561 tggcccgata atgccgtagc ggttgcggct tggcaccatg tagaccggtt tcgcgcctgt
     1621 cagcatcaag ccttgttcga tggatttatg gcagttacgg tcaacgacca cgacatcgtt
     1681 atcggtcatg caagcctgca tgatggtgcg gttagagccg gaagtaccga cgactaccga
     1741 ccaggagcga tcggcaccaa atacgcgtgc ggcatatttt tcgctttcgc caaatgcgcc
     1801 agtatggtca agcaaagaac cgagggaagt tcgttcgatg cccatatcgg tgcggaacag
     1861 attttcacca tagtagtcat ggtagaaacg tccggcgggt gttttggtaa aaccaacgcc
     1921 gccctggtgg cctggcgctg cccaggaata ttcatggatg tcgctatatt tcatcagcgc
     1981 gctgaacagt ggcggcaaca gctgctggcg gtagcgggtc atcgcggcaa cagcgcgtcc
     2041 ggcgataaag tcggcggtat cttccagaat ccaggcgaat tcatcgacaa gctccagcag
     2101 gtcgcgatcc attgcggcga gggctttttc ccgatcgccc aacaggaaga ccggcacgtt
     2161 ttgttggcgc tcatgaagct taccgatcaa ttgtctgacg ttttgatgtt cgtccggatg
     2221 ttccatttga tagctgaaca tcaggcagtc aatggcttcg tttgaagaga gaatggcaaa
     2281 accatcatca aaggaggtgg atttaatcac ggtaacattt tgctggctta aagcatctgc
     2341 cagacgctca acggcgttac cgacccaggt gtcttgatgg agaaactcgc tttcaacaat
     2401 taatactttc atcattgctt acccggttat gaaggaatct tcgttgcggg caagtatctt
     2461 ccggcctttt cgggctgtaa agcgcgtgaa aaacaaacat ttttgaaata tttattttta
     2521 taaacaatgc gttatgtgat tattttaaat tattttcact gcttattatc gacggctaaa
     2581 ctattttttt ggctgatact gatatcgtct ttagccggga aacgtctggc ggcgctgttg
     2641 gctaagtttg cggtattgtt gcggcgacat gccgacatat ttgccgaacg tgctgtaaaa
     2701 acgactactt gaacgaaagc ctgccgtcag ggcaatatcg agaatacttt tatcggtatc
     2761 gctcagtaac gcgcgaacgt ggttgatgcg catcgcggta atgtactgtt tcatcgtcaa
     2821 ttgcatgacc cgctggaata tccccattgc atagttggcg ttaagtttga cgtgctcagc
     2881 cacatcgttg atggtcagcg cctgatcata gttttcggca ataaagccca gcatctggct
     2941 aacataaaat tgcgcatggc gcgagacgct gtttttgtgt gtgcgcgagg ttttattgac
     3001 cagaatcggt tcccagccag agaggctaaa tcgcttgagc atcaggccaa tttcatcaat
     3061 ggcgagctgg cgaatttgct cgttcggact gtttaattcc tgctgccagc ggcgcacttc
     3121 aaacgggcta agttgctgtg tggccagtga tttgatcacc atgccgtgag tgacgtggtt
     3181 aatcaggtct ttatccagcg gccaggagag aaacagatgc atcggcagat taaaaatcgc
     3241 catgctctga caggttccgg tatctgttag ttggtgcggt gtacaggccc agaacagcgt
     3301 gatattaccc tgattgatat tcactttttc attgttgatc aggtattcca catcgccatc
     3361 gaaaggcaca ttcacttcga cttgaccatg ccagtggctg gtgggcatga tatgcggtgc
     3421 gcgaaactca atctccatcc gctggtattc cgaatacagc gacagcgggc tgcgggtctg
     3481 tttttcgtcg ctgctgcaca taaacgtatc tgtattcatg gatggctctc tttcctggaa
     3541 tatcagaatt atggcaggag tgagggagga tgactgcgag tgggagcacg gttttcaccc
     3601 tcttcccaga gaggcgaggg gagcgtccga gcatcatgag gccgaaaact ctgcttttca
     3661 ggtaacttat taccataaac tcagatttac tgctgcttca cgcaggatct gagtttatgg
     3721 gaatgctcaa cctggaagcc ggaggttttc tgcagattcg cctgccatga tgaagttatt
     3781 caagcaagcc aggagatctg catgatgact gcacccaaaa ttacatttat cggcgctggt
     3841 tcgacgattt tcgttaaaaa tattcttggt gatgtgttcc atcgcgaggc gctgaaaacg
     3901 gcgcatattg ccctgatgga cattgatccc acccgcctgg aagagtcgca tattgtggtg
     3961 cgtaagctga tggattcagc aggggccagc ggcaaaatca cctgccacac ccaacagaaa
     4021 gaagccttac aggatgccga ttttgtcgtg gtggcatttc agattggcgg ttatgaacct
     4081 tgcacggtga ctgatttcga ggtctgtaag cggcatggtc tggaacaaac cattgccgat
     4141 acgttggggc cgggcggtat tatgcgcgcg ctacgtacca ttccgcatct gtggcaaatt
     4201 tgcgaggaca tgacggaagt ctgccccgat gccaccatgc tcaactacgt taacccaatg
     4261 gcgatgaata cctgggcgat gtatgcccgc tatctgcata tcaaacaggt agggctgtgc
     4321 cattcggtgc agggaacggc ggaagagctg gcgcgtgacc tcaatatcga tccgtccacg
     4381 ctgcgttacc gttgcgctgg tatcaaccat atggcgtttt acctggaact ggagcgtaaa
     4441 accgccgacg gcagttacgt gaatctctac ccggaactgc tggcggctta tgaagcaggg
     4501 caggcaccga agccaaatat tcatggtaat actcgctgcc agaatattgt gcgctacgaa
     4561 atgttcaaaa agctgggcta cttcgttacg gaatcgtcag aacattttgc tgaatacacg
     4621 ccgtggttta ttaagccagg tcgtgaggat ttgattgagc gttataaagt accgctggat
     4681 gagtacccga aacgctgcgt cgagcagctg gcgaactggc ataaagagct ggaggagtat
     4741 aaaaacgcct cccggattga tattaaaccg tcacgagaat atgccagcac aatcatgaac
     4801 gctatctgga ctggcgagcc gagtgtgatt tacggcaacg tccgtaacga tggtttgatt
     4861 gataacctgc cacaaggatg ttgcgtggaa gtagcctgtc tggttgatgc taatggcatt
     4921 cagccgacta aagtcggtac gctaccttcg catctggccg ccctgatgca aaccaacatc
     4981 aatgtacaga cgctgctgac cgaagctatt cttacggaaa atcgcgaccg tgtttaccac
     5041 gccgcgatga tggacccgca tactgccgcc gtgctgggca ttgacgaaat atatgctctt
     5101 gttgacgacc tgattgccgc ccacggcgac tggctgccag gctggttgca ccgttaaaac
     5161 gcgactaaac gctactgcgc cgggggattt attccggcgc acacctctga cgataccaat
     5221 aacagaaggc gggcgttggt aacagcgacc cgatacccta tgagcatttc aatgactaca
     5281 aaactcagtt atggatttgg agcgttcggg aaggattttg cgatcggcat tgtgtatatg
     5341 tacctcatgt attactacac cgatgtcgtt gggctgtctg tgggtttggt cggtactttg
     5401 tttctggtgg cgaggatctg ggatgctatt aacgatccga ttatgggatg gattgtaaat
     5461 gctacgcgat cgcgatgggg taagttcaaa ccctggatcc tgatcggtac gttggcaaac
     5521 tctgtaatct tatttctcct ctttagtgcg catctgtttg aaggtactac tcagattgtc
     5581 tttgtttgcg tgacctacat cctctggggc atgacttaca ccattatgga tattcccttc
     5641 tggtcgctgg ttccaaccat cacgctcgat aaacgtgagc gtgaacaact ggttccttat
     5701 ccgcgttttt ttgccagtct ggcgggcttt gttacggcag gtgtgacgct accatttgtt
     5761 aattatgtcg gcggtggcga tcggggattt ggctttcaga tgttcacact ggtactgatc
     5821 gcctttttta ttgtttcaac catcatcact ctgcgcaatg tgcatgaagt cttttcgtca
     5881 gacaatcaac cgtctgctga aggaagccat ctgacactta aagccatcgt tgcgctaatt
     5941 tataaaaacg atcagctttc atgcctcttg ggtatggctc ttgcttataa tgtagccagc
     6001 aacattatta ccggatttgc tatctattat ttctcatatg ttatcggtga tgcggatttg
     6061 ttcccctatt atctgtcgta tgcgggagct gctaacctgg tgacgtta
//
LOCUS       DADMKF010000202         6073 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.206, whole genome shotgun sequence.
ACCESSION   DADMKF010000202 DADMKF010000000
VERSION     DADMKF010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6073
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.206"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            235..1341
                     /gene="ompC"
                     /locus_tag="JNC24_003744"
     CDS             235..1341
                     /gene="ompC"
                     /locus_tag="JNC24_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HAY9160392.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNKSEDGDQTYVRLGFKGVTQVTDQLTGYGQWEYQIQGNTSEDNKENSWTRVAFAGL
                     KFQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRNTDFFG
                     LVDGLNFAVQYQGKNGSVSGEGMTNNGRGALRQNGDGVGGSITYDYEGFGIGGAISSS
                     KRTDDQNSPLYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGSLGWANKAQN
                     FEAVAQYQFDFGLRPSVAYLQSKGKNLGTIAGRNYDDEDILKYVDVGATYYFNKNMST
                     YVDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            1453..2508
                     /gene="apbE"
                     /locus_tag="JNC24_003745"
     CDS             1453..2508
                     /gene="apbE"
                     /locus_tag="JNC24_003745"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HAY9160393.1"
                     /translation="MEISFTRVALLAAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGTKTDGAMDITVGPLVNLWGFGPEQQSVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLNVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGLPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLISEKN"
     gene            2582..3646
                     /gene="ada"
                     /locus_tag="JNC24_003746"
     CDS             2582..3646
                     /gene="ada"
                     /locus_tag="JNC24_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HAY9160394.1"
                     /translation="MKNATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANPRQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            3646..4296
                     /gene="alkB"
                     /locus_tag="JNC24_003747"
     CDS             3646..4296
                     /gene="alkB"
                     /locus_tag="JNC24_003747"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HAY9160395.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILRRFAFNAAEQLIRDINDVASQS
                     PFRQMVTPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHDLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTGDCRYNLTFRQAGKKE"
     gene            4372..6015
                     /gene="yojI"
                     /locus_tag="JNC24_003748"
     CDS             4372..6015
                     /gene="yojI"
                     /locus_tag="JNC24_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HAY9160396.1"
                     /translation="MELLVLVWRQYRWPFISVMELSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAITLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELVQGIILTIGSAAYLWMLSGKMLLVTAIWMAVT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFSYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGQPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAADQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
BASE COUNT         1441 a         1534 c         1646 g         1452 t
ORIGIN      
        1 tgatgttagg tgcttatttc gccattccgc aataatctta aaaagttccc ttgcatttac
       61 attttgaaac atctatagcg ataaatgaaa catcttaaaa gttttagtat catattcgtg
      121 ttggattatt ctgcattttt ggggagaatg gacttgccga ctgattaatg agggttaatc
      181 agtatgcagt ggcataaaaa agcaaataaa ggcatataac agagggttaa taacatgaaa
      241 gttaaagtac tgtccctcct ggtcccagct ctgctggtag caggcgcagc aaacgctgct
      301 gaagtttaca acaaagacgg caacaaatta gatctgtacg gtaaagtaga cggcctgcac
      361 tatttctctg acaacaagtc agaagacggc gaccagacct atgtacgtct tggtttcaaa
      421 ggcgtaactc aggttactga ccagctgacc ggttacggcc agtgggaata tcagatccag
      481 ggcaatacct ctgaagacaa caaagaaaac tcctggaccc gtgtggcatt cgcaggtctg
      541 aaattccagg atgtgggttc tttcgactac ggtcgtaact acggcgttgt ttacgacgta
      601 acttcttgga ccgacgtact gccagaattc ggtggcgaca cctacggttc tgacaacttc
      661 atgcagcagc gtggtaacgg cttcgcgacc taccgtaaca ctgacttctt cggtctggtt
      721 gacggcctga actttgctgt tcagtaccag ggtaaaaacg gtagcgtaag cggcgaaggc
      781 atgaccaaca acggtcgtgg tgctctgcgt cagaacggcg acggcgttgg cggatctatc
      841 acttatgatt acgaaggctt cggtatcggt ggtgcgatct ccagctccaa acgtactgat
      901 gatcagaaca gcccgctgta catcggtaac ggcgaccgtg ctgaaaccta caccggtggt
      961 ctgaaatacg acgctaacaa catctacctg gctgctcagt acacccagac ctataacgca
     1021 actcgcgtag gttccctggg ttgggcgaac aaagcacaga actttgaagc tgttgctcag
     1081 taccagttcg acttcggtct gcgtccgtct gtagcatacc tgcagtctaa aggtaaaaac
     1141 ctgggtacca tcgctggtcg taactacgac gacgaagata tcctgaaata tgttgatgtt
     1201 ggtgctacct actacttcaa caaaaacatg tccacctacg ttgactacaa aatcaacctg
     1261 ctggacgaca accagttcac tcgtgacgct ggcatcaaca ctgataacat cgtagctctg
     1321 ggtctggttt accagttcta atctcgattg atatcgaaca aagggcctgc gggccctttt
     1381 ttcattgttt tcagcgtaca aactcatttt tttggtgtac tcttgcgacc gttcgcatga
     1441 ggataatcac gtatggaaat aagctttacc cgcgtggcac tgctggctgc cgcgctcttc
     1501 tttgttggtt gcgatcaaaa accacaaccc gccaaaaccc acgctactga agttaccgtt
     1561 ctagaaggca aaaccatggg taccttctgg cgtgccagca tcccgggcat tgatgccaaa
     1621 cgcagtgccg aacttaaaga aaagattcag acccagctgg acgccgacga tcagctgctt
     1681 tcgacctata aaaaagattc cgcgctgatg cgctttaacg attcgcaaag tttatcgccg
     1741 tggccggtaa gtgaagcgat ggccgatatc gtcaccacct cgctgcgcat tggcacgaag
     1801 accgatggcg cgatggatat aaccgtcggg ccgctggtga atctgtgggg ctttggcccg
     1861 gaacaacagt cggttcaaat tccgagccag gaacagatcg atgcgatgaa agccaaaacc
     1921 ggcttacagc acctgacggt cattaatcag tcgcatcagc aatatctgca aaaagacctg
     1981 ccggatttaa atgtcgatct ctccaccgtc ggtgaaggtt atgcggcgga tcacctggca
     2041 cgcttgatgg agcaggaagg gatttcccgc tatctggtgt cggtgggcgg cgcgctgaac
     2101 agccgtggta tgaacggtga aggcctgccg tggcgggtag cgattcaaaa accaaccgat
     2161 aaagaaaacg cggttcaggc tgtggtggat atcaacggtc atgggattag cacctctggc
     2221 agctaccgta actattacga actggacggc aaacgtcttt cccatgttat cgatccgcaa
     2281 accgggcgtc ccatcgaaca caatctggta tccgtgacgg tgattgctcc gacggcgctg
     2341 gaagccgatg cctgggatac tggcttgatg gtactcgggc cggagaaagc caaagaggtt
     2401 gttcgccggg aagggctggc ggtttatatg atcaccaaag aaggcgatag ctttaaaacc
     2461 tggatgtcac cacagtttaa aagcttcctt atcagcgaaa aaaattaaag cgcaagattg
     2521 ttggtttttg cgtgatggtg accgggcaga ctaaaggcta tccttaacca gggagctgat
     2581 tatgaaaaac gccacatgct taactgacga tcaacgctgg caatctgtct tagcccgcga
     2641 cccgaatgcc gacggcgaat tcgttttcgc cgtgcgcacc acgggtatct tttgccgtcc
     2701 gtcttgccgc gccagacatg ccttgcggga aaatgtctcc ttctacgcaa atgccagcga
     2761 ggcactcgcc gccggatttc gcccctgcaa acgttgtcag ccagacaaag ccaatccccg
     2821 gcaacatcgg ttggataaaa tcacccacgc gtgtcgactg ctggaacagg aaacgcctgt
     2881 aacgctggaa gccttagccg accaggtggc gatgagtcca tttcatctac atcggttgtt
     2941 taaagcgact accggaatga cgcctaaagc ctggcaacag gcctggcgcg ctcgccgttt
     3001 gcgcgaatcg ctggcgaaag gggagagcgt gacgacgtct attcttaacg ccggattccc
     3061 cgacagcagc agttactacc gcaaagccga tgaaacgctg ggcatgacgg ctaaacaatt
     3121 ccgtcacggt ggcgaaaatc tggcggtgcg ttacgcgctg gctgattgtg agctgggtcg
     3181 ttgcctggtg gcagaaagcg agcgggggat ttgcgcgata ttgctgggcg atgatgacgc
     3241 gacattaatc agcgagttgc agcagatgtt tcccgctgcc gacaacgcgc ctgccgatct
     3301 gacgtttcag caacatgtgc gtgaagtgat cgccagcctc aatcaacgcg atacgccgct
     3361 gacgttaccg ctggacattc gcggcactgc ttttcagcaa caagtctggc aggcactgcg
     3421 cacgatacct tgtggtgaaa ccgtcagtta tcagcaactg gctaacgcca tcggcaaacc
     3481 gaaagcggta cgggccgttg ccagcgcctg tgccgccaac aagctggcta tcgtaatacc
     3541 ctgtcatcga gtggtccgtg gtgatggcac actttccggt taccgctggg gcgtgtcgcg
     3601 taaagcgcaa ctgctgcgcc gcgaagctga aaatgaggag aggtaatgtt ggatctgttt
     3661 gccgatgctg aaccgtggca agagccactg gcggctggtg cggtaatttt acggcgtttt
     3721 gcttttaacg ctgcggagca actgatccgc gatattaatg acgttgccag ccagtcgccg
     3781 tttcgccaga tggtcacccc cgggggatat accatgtcgg tggcgatgac caactgtggg
     3841 catctgggct ggacgaccca tcggcaaggt tatctctatt cgcccattga tccgcaaaca
     3901 aataaaccgt ggcccgccat gccacaaagc tttcatgatt tatgtcaacg tgcggctacg
     3961 gcggcgggct atccagattt ccagccagat gcttgtctta tcaaccgcta cgctcctggc
     4021 gcgaaactgt cgctgcatca ggataaagac gaaccggatc tgcgcgcgcc gattgtttct
     4081 gtttctcttg gcttacccgc gatttttcaa tttggtggcc tgaaacgtaa tgatccgctc
     4141 aaacgtttgt tgttggaaca tggcgatgtg gtggtatggg gcggtgaatc gcggctgttt
     4201 tatcacggca ttcaaccgct gaaagcgggg tttcatccac tcaccggcga ctgccgttac
     4261 aacctgacat tccgtcaggc aggtaaaaaa gaataaaaat aagaattatt attgctgtgc
     4321 gcgcgaagat gtttaaactg cgggctgtaa ttcattgtcc gggttttctg catggaactt
     4381 cttgtacttg tctggcggca gtatcgctgg ccatttatca gtgtgatgga gctaagcctc
     4441 gccagtgcgg ctttaggcat tggcttaatt gcttttatca atcagcgcct tatcgaaacg
     4501 gcggatacca gtctgctggt gttgccggag tttctgggat tattgctgct gttgatggca
     4561 atcactctcg gatcgcaact ggcgctcacc actttggggc atcacttcgt ttaccgactg
     4621 cgtagtgaat ttatcaagcg gattctcgat acccacgtcg agcgcattga acaactcggt
     4681 agcgcctcgt tgctggcggg attaaccagc gatgtgcgca atatcaccat tgcttttgtg
     4741 cgtctgccgg aactggtgca ggggatcatt ctcactatcg gttcagcggc gtatctgtgg
     4801 atgctgtcgg gcaaaatgtt gctggtaacg gctatctgga tggcggtcac catctggggc
     4861 ggttttgtgc tggtggcgcg ggtgtacaaa catatggcga ccctgcgtga aaccgaagat
     4921 aagctgtaca cggattttca aaccgtactc gaagggcgca aagagctgac tctgaaccgt
     4981 gaacgcgccg agtatgtgtt taacaacctc tacattcctg atgcgcaaga gtatcgccat
     5041 catattatcc gcgcagacac cttccatctt agtgccgtga actggtcaaa catcatgatg
     5101 ctgggcgcta tcggcctggt gttctggatg gcgaacagcc tcggttgggc tgataccaac
     5161 gttgctgcga cctattcgtt gacgctgtta ttcctgcgta cgccgctgct ttcggcggtt
     5221 ggcgcattgc cgacgctgct gacggcgcag gtggcgttta acaagctgaa caaattcgcg
     5281 ctcgcgcctt tcaaagcaga gtttccgcgc ccgcaggcgt ttcccaactg gcaaacgctg
     5341 gagctgcgta atgtgacgtt tagctatcag gataacgcgt tttccgttgg gccgattaac
     5401 ctcaccatta aacgtggcga gctgctgttt ctgattggtg gcaacggtag tggaaaatcg
     5461 acgctggcga tgttgctgac gggcttgtac cagccacaaa gcggcgaaat cttgctggat
     5521 ggcaaacctg tcagcggcga acaaccggaa gattatcgca aactgttttc ggcagtgttt
     5581 accgatgtct ggctgtttga tcaactgctg gggccggagg ggcaacccgc taacccgcaa
     5641 ctggttgaga agtggctggc gcagctgaaa atggctcata agcttgagtt aagcaacggg
     5701 cgtattgtta acctgaagtt atcaaaaggg cagaaaaaac gcgtggcgct gttgctggcg
     5761 ctggcagaag aacgcgatat tatcctgctg gatgaatggg cggcggatca ggatccacac
     5821 ttccgtcgtg agttttatca ggtgttgctg ccgctgatgc aggagatggg taaaactatt
     5881 ttcgccatca gtcatgatga tcattacttt atccacgccg accgcctgct ggaaatgcgc
     5941 aatgggcaac ttagcgagct gacgggcgaa gagcgcgatg ccgcttcgcg tgatgccgtt
     6001 gcccggacgg cataatatta cgacgctgac ctggcggact gctgccgcca ggtcaatatt
     6061 ctaaaaatcc ctt
//
LOCUS       DADMKF010000203         5934 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.207, whole genome shotgun sequence.
ACCESSION   DADMKF010000203 DADMKF010000000
VERSION     DADMKF010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5934
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.207"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            292..368
                     /locus_tag="JNC24_003749"
     tRNA            292..368
                     /locus_tag="JNC24_003749"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:326..328,aa:Arg,seq:tct)
     gene            complement(413..1174)
                     /locus_tag="JNC24_003750"
     CDS             complement(413..1174)
                     /locus_tag="JNC24_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAY9160397.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLITHINRNIINDYLLFLNKNLTCVKPWSRLATPVIACHSRTPEVFRLAA
                     NHSKQQPSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENISYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYGLTPLNYLAKQRQKVMW"
     gene            complement(1357..2247)
                     /locus_tag="JNC24_003751"
     CDS             complement(1357..2247)
                     /locus_tag="JNC24_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HAY9160398.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRLQGFDTLVLQWTRYGDAFTQPEQRTLLFKRAAAAQQAGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGITPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDTLYFSLRYLPVAHGILEY"
     gene            complement(2248..5220)
                     /gene="nfrA"
                     /locus_tag="JNC24_003752"
     CDS             complement(2248..5220)
                     /gene="nfrA"
                     /locus_tag="JNC24_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
                     /protein_id="HAY9160399.1"
                     /translation="MKENNLNRVIGWSGLLLTSLLSTSALADNIGTSAEELGLSDYRH
                     FVIYPRLDKALKAQKNNDEATAIREFEYIHQQVPDNIPLTLYLAEAYRHFGHDDRARL
                     LLEDQLKRHPGDARLERSLAAIPVEVKSVTTVEELLAQQKACDAAPTLRCRSEVGQNA
                     LRLAQLPVARAQLNDATFAASPEGKTLRTDLLQRAIYLKQWSQADTLYNEARQQNTLS
                     AAERRQWFDVLLAGQLDDRILALQSQGIFTDPQSYITYATALAYRGEKARLQHYLNEN
                     KPLFTTDAQEKSWLYLLSKYSANPVQALANYTVQFADNRQYVVGATLPVLLKEGQYDA
                     AQKLLATLPANEMLEERYAVSVATRNKAESLRLARLLYQQEPANLTRLDQLTWQLMQN
                     EQSREAADLLLQRYPFQGDARVSQTLMARLASLLESHPYLATPAKVAILSKPLPLAEQ
                     RQWQSQLPGIADNCPAIVRLLGDMSPSYDAAAWNRLAKCYRDTLPGVALYAWLQAEQR
                     QPSAWQHRAVAYQAYQVEDYATALAAWQKISLHDMSNEDLLAAANTAQAAGNGAARDR
                     WLQQAEQRGLGSNALYWWLHAQRYIPGQPELALNDLTRSINIAPSANAYVARATIYRQ
                     RHNVPAAVSDLRAALELEPNNSNIQAALGYALWDSGDIAQSREMLEQAHKRLPDDPAL
                     IRQLAYVNQRLDDMPATQHYARLVIDDIDNQALITPLTPEQNQQRFNFRRLHEEVGRR
                     WTFSFDSSIGLRSGAMSTANNNVGGAAPGKSYRSYGQLEAEYRIGRNMLLEGDLLSVY
                     SRVFADTGENGVMMPVKNPMSGTGLRWKPLRDQIFFLAVEQQLPLNGQNGASDTMLRA
                     SASFFNGGKYSDEWHPNGSGWFAQNLYLDAAQYIRQDIQAWTADYRVSWHQKVANGQT
                     IEPYAHVQDNGYRDKGTQGAQLGGVGVRWNIWTGETHYDAWPHKVSLGVEYQHTFKAI
                     NQRNGERNNAFLTIGVHW"
     gene            complement(5207..>5934)
                     /locus_tag="JNC24_003753"
     CDS             complement(5207..>5934)
                     /locus_tag="JNC24_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9160400.1"
                     /translation="LDTALRNRVEGLRLGGSMLMQGLISAEQLAQALAEQNGVAWESI
                     DAWQIPSSLIAEMPASVALHYAVLPLRLENDELIVGSEDGIDPVSLAALTRKVGRKVR
                     YFIVLRGQIVTGLRHWYARRRGHDPRAMLYNAVQHQWLTEQQAGEIWRQYVPHQFLFA
                     EILTTLGHINRSAINVLLLRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSMQ
                     SLLLKAGLNTEQVAQLESENEGE"
BASE COUNT         1347 a         1559 c         1551 g         1477 t
ORIGIN      
        1 ttctgatgat aggatgagta tccataatga tcaccgacgt tggtttcata gttaccagtc
       61 tcataggagc ggacaatttt ccgttagggc ggtaaattgt actttgatac atgaaaatac
      121 gggttttctt gattcaaacg cgcagcggtg tgcatttgtt tgccgctata gcgtaataaa
      181 tcagaaaatc agacgcggtc gttcacttgt tcagcaacca gatcaaaagc cattgactca
      241 gcaagggttg accgtataat tcacgcgatt acaccgcatt gcggtatcaa cgcgccctta
      301 gctcagttgg atagagcaac gaccttctaa gtcgtgggcc gcaggttcga atcctgcagg
      361 gcgcgccatt tcttcttcat ttaaccccgt cttatccgtt ccgctttgct cttcaccaca
      421 tcactttttg tcgctgtttg gcgagataat tcaacggtgt caggccgtaa aacgccttaa
      481 aaacagagat aaagtacgac gtgctgctat agccacataa ttgcgccacc tgagtgatat
      541 ttttgttatc catcaataac atctgtacgg cgtaacgcat acgacactct gtgacaatct
      601 ggctatagct gatattttcg ttttttaatt tctttttgag caggctgggg cttaaacata
      661 gcgaactggc gacaattcgc agattccagt aatgctgaat atcgctttga ataatgcggc
      721 agacgctgtc gcggacgctg ctgcgtaaga tatacatcag tagggcaata aaccgcgatt
      781 gctcaagaaa gttagacaat acggtaaaaa gcaatgcgcg cgtcaactcc gcttcgcagg
      841 gtttgctggg ttgctgcttg ctgtggttgg cggctagccg gaacacttcc ggtgtacggc
      901 tatgacaagc gataaccggg gttgccagcc gcgaccaggg ctttacacag gttaagttct
      961 tatttaaaaa caacagataa tcgttgatga tgttacggtt gatgtgggtg attaatacgt
     1021 gatttaacgt tgagagttca atgacgttat tgttgcacgc gaaggcggcc aggtagtttt
     1081 tcggaagggt aaacgtagca tggttattga cgcttacctc tacctctttt tcggtcagga
     1141 tcaccacgca aggttcactg ctgctcaatt gcatttcgca ctcctcagat atcagaaact
     1201 ccgctcaaag gatctatgct tcctgcatga gtgatcggcc cgttcgccga taacgatctt
     1261 ctttctttag cacgcttttt agcaattaat cttgatggaa ttctgacgag agcgaaagag
     1321 gtaagccagg tcgtacccga cttacctgga ggagatttaa tactcgagaa tgccgtgcgc
     1381 gacgggcaaa tagcgcagag agaaatagag agtgtcttta ccgcaagagg aacgtttcgc
     1441 taacagcgag gcgatctctg catccggttt cggtttcgct gtaaaggttt tgcctttgcc
     1501 ggcgacaaac agttcataaa caatgccgct tgctggggcg gagctttggc aatcggcgct
     1561 ggcctgtaga taacgttcac gctgttcagc ggtaagttta tccacgccgc tgccatcctg
     1621 tacccagaca ttaactccgg ttgctttaac ttgttccagc agttggcgat agccatctgg
     1681 cgacatgttt ccggcgaaaa aactactgat ataaaccggt tttgctgaaa catcgctaat
     1741 cagccgctgc gcgttgttta accatgttag caaaggctga cgggcagctt cgctgcgcca
     1801 gttcaggtcg tcaatttccg cgctgatgta ccagccatcc ggcgttatgc caggcgcggc
     1861 gctccataat ctggcttgct ggagatcggc agccagcagg cgattaagat agctttccag
     1921 cgctgcggac gactgtttct ggtgcataaa aaattccgga tcggcgttca gcccgacaat
     1981 cagtttcagg ccagcctgtt gcgcagctgc ggcacgctta aacaataacg tgcgctgttc
     2041 tggctgggta aatgcatcgc cgtaacgggt ccattgcaaa accagggtat cgaagccttg
     2101 caaacgtaac tgactcatca gcccctgcca ctgggtatcg gtaacctgac tatctcggtt
     2161 ttgtggttgc cagataatac ctttcatcgc aaaggaaaaa gggctgacca aaagcagtgt
     2221 cagcaatacg aaaatgaact tacgcattta ccagtgcact ccaatggtga gaaacgcgtt
     2281 gttgcgctct ccgttacgtt gattaatcgc cttaaaggta tgttgatact cgacgccgag
     2341 actgactttg tgcggccagg cgtcgtagtg cgtctcgccg gtccagatat tccagcggac
     2401 cccgactccg ccaagctgcg cgccctgagt gcctttatca cgatagccgt tgtcctgaac
     2461 gtgagcgtaa ggctcaatag tctgtccgtt agctaccttc tgatgccagc tgacgcgata
     2521 atctgccgtc cacgcctgaa tatcctggcg gatatattgc gccgcatcga ggtacaggtt
     2581 ttgggcaaac cagcctgaac cgttcgggtg ccattcgtcg ctgtatttgc cgccattaaa
     2641 gaatgaggcg ctggcgcgca gcatggtatc ggatgcgcca ttttgcccgt tcagcggcaa
     2701 ctgctgttcg acggcgagga aaaagatctg atcgcgcagc ggcttccagc gcagaccggt
     2761 gccggacatc ggatttttca ccggcatcat caccccgttt tctccggtat cggcaaagac
     2821 gcggctataa actgagagca ggtcgccttc cagcagcata ttgcgtccga tgcggtactc
     2881 ggcttccagt tgcccgtagc tacgatagct tttccctggc gctgcgccgc caacattatt
     2941 gttagcggta ctcattgccc cggaacgcaa gccgatagaa gaatcgaaac tgaacgtcca
     3001 gcggcgaccg acctcctcat gcaaacggcg gaaattgaag cgttgttgat tttgctctgg
     3061 ggtcagtggg gttatcagag cctgattatc aatgtcatca atcaccagcc gggcgtagtg
     3121 ctgcgtcgca ggcatgtcat ccagacgctg gttcacgtag gccagttgtc ggatcagtgc
     3181 cggatcgtcc ggaagccttt tatgcgcctg ttcgagcatt tcccgcgact gtgcgatatc
     3241 accgctatcc cacaaggcgt aaccaagcgc tgcctggatg ttgctattat tcggttccag
     3301 ttccagcgcg gcgcgcaaat cactcaccgc ggccgggaca ttatgacgtt ggcgataaat
     3361 tgtcgcccgc gcaacgtaag cgttggcaga aggcgcaata ttgattgagc gcgtgagatc
     3421 gttcagtgcg agttccggct gaccaggaat gtaacgttgt gcatgcagcc accagtagag
     3481 ggcattgctt cccagtccac gttgttctgc ctgttgtagc cagcgatcgc gagccgcacc
     3541 atttcctgcc gcctgggcgg tattggcagc agcaagcaga tcctcattgc tcatgtcgtg
     3601 aagactgatt ttctgccagg ccgccagtgc ggtggcgtag tcctcaacct gatacgcctg
     3661 ataggctacc gcacgatgtt gccaggcgct cggttgtcgt tgttcggcct gaagccatgc
     3721 atacaacgcc acaccgggta gcgtgtcccg ataacacttt gccagacggt tccaggcggc
     3781 agcatcgtag gaaggcgaca tatcgcccag caagcgaact attgccgggc aattatctgc
     3841 aatacccggc aactgacttt gccactgacg ttgctccgcc agcggtaagg gtttcgataa
     3901 aatcgccacc ttcgccggcg ttgccaggta aggatgactt tccagcagag acgccagtcg
     3961 cgccattaaa gtctggctga cacgcgcatc gccctggaaa ggatagcgtt gcagcaataa
     4021 atcggcagct tcgcgtgact gctcgttctg catcagttgc caggtcagtt gatccaggcg
     4081 ggtaagattt gccggttctt gctgatacag caatcgtgcc agacgcagag attcagcctt
     4141 gttacgggtc gccacgctga cagcataacg ctcctcaagc atttcattgg cggggagggt
     4201 ggcgagcagt ttttgcgctg cgtcgtactg accttctttt aacagcaccg gtagcgtcgc
     4261 gccaacaaca tactggcggt tatcggcaaa ctgcaccgta taattcgcca atgcctgaac
     4321 ggggttggcg ctgtatttag ataacagata gagccaactt ttctcttgtg cgtccgtggt
     4381 aaacagcggc ttattttcat tgagataatg ctggaggcgt gctttttcgc cacgataagc
     4441 cagcgcggtc gcgtaagtaa tatatgactg aggatcggtg aagatcccct gtgattgcag
     4501 tgctaggatc cgatcgtcca gctgcccggc aagaagcacg tcaaaccact gacggcgttc
     4561 tgccgcgctt aatgtgttct gctggcgtgc ttcattgtat agcgtatctg cctgggacca
     4621 ttgtttcagg tagattgccc gttgcagcag atcggttcgc agcgtttttc cttccggcga
     4681 tgcagcaaac gtcgcatcgt tcagttgcgc tctggcgaca ggtaactgtg caagccgcag
     4741 ggcattctgc ccgacttcac tgcgacaacg cagggtcggc gcagcatcgc acgctttttg
     4801 ctgggcaagc agttcttcaa cagttgtaac gcttttcact tcaaccggaa tagccgccag
     4861 actgcgctca agtcgggcat ctcctgggtg acgtttcagt tgatcctcaa gcaacagccg
     4921 cgcccggtca tcatgaccaa aatggcgata ggcttccgca aggtataaag tcagcggaat
     4981 attatccggc acctgctggt gtatatattc aaattcgcgg atggcggttg cttcgtcgtt
     5041 atttttctgt gccttcagcg ccttatcgag acggggataa ataacaaaat ggcgataatc
     5101 gctcagcccc agctcttctg cgctggtgcc gatattgtct gcgagtgcgc tggtactcaa
     5161 taaagacgtc agcagtaaac cagaccatcc gatgacgcga ttaaggttat tctccttcat
     5221 tttcggactc cagttgcgca acctgttctg tgtttaaacc tgctttgagt aatagtgatt
     5281 gcatcgaaac ttgtaattcg cgttgaattg tcaggacgcg atccaacgtt tcctggctga
     5341 taacgccttc ggtgaccaaa aacttgccga gcggcagaga actgcgttca tggcgcaata
     5401 acaacacgtt aattgctgaa cgattaatat gaccgagcgt ggtcagtatt tcggcgaaca
     5461 ggaactgatg cggcacatat tgccgccaga tttcaccggc ctgctgttcc gtgagccact
     5521 gatgctgaac cgcattgtac aacattgccc gcggatcgtg accgcgtcgg cgtgcatacc
     5581 agtgacgtaa ccccgtgaca atttgtcccc gcagaacaat gaagtaacgc actttgcgtc
     5641 cgactttacg cgtcagggcc gccagcgaaa ccgggtcaat accatcttca ctgccgacaa
     5701 ttaactcatc attttccaga cgcagcggca gtaccgcata atgcagcgcc acggaggccg
     5761 gcatttcggc aatcagcgag gaagggatct gccaggcatc gatggattcc cacgccacgc
     5821 cgttttgctc tgccagcgcc tgtgccagct gctcggcgct aatcagcccc tgcatcagca
     5881 ttgaaccgcc caagcgtaga ccttcgacgc gattacgcag tgctgtatcg agtt
//
LOCUS       DADMKF010000204         5640 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.208, whole genome shotgun sequence.
ACCESSION   DADMKF010000204 DADMKF010000000
VERSION     DADMKF010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5640
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.208"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(33..770)
                     /gene="bamD"
                     /locus_tag="JNC24_003754"
     CDS             complement(33..770)
                     /gene="bamD"
                     /locus_tag="JNC24_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HAY9160401.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            905..1885
                     /gene="rluD"
                     /locus_tag="JNC24_003755"
     CDS             905..1885
                     /gene="rluD"
                     /locus_tag="JNC24_003755"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HAY9160402.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            1882..2613
                     /gene="pgeF"
                     /locus_tag="JNC24_003756"
     CDS             1882..2613
                     /gene="pgeF"
                     /locus_tag="JNC24_003756"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase"
                     /protein_id="HAY9160403.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAVHAGWRGLCAGVLEETVSCFADKPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            2743..5316
                     /gene="clpB"
                     /locus_tag="JNC24_003757"
     CDS             2743..5316
                     /gene="clpB"
                     /locus_tag="JNC24_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HAY9160404.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
BASE COUNT         1401 a         1351 c         1529 g         1359 t
ORIGIN      
        1 caagggctgc cgttttgtgt ttcaggtttc tgttatgtat tgctgctgtt tgcggcgatg
       61 attttcgcta ctttttcagc ttgcgcattc atctgcatct gacggtatgc attttccatc
      121 agtggcagcg catcacgcgt agcctgggta tccgggtagt cgcgcaacat gccttctacg
      181 cggttaacga cggcaaccca tgcaccacgt tctgtatagt actcggcaac ggagtattca
      241 tatttcgcca gacgatcttt caggaatacc agacgtttgg tggcatcggt agtgtactga
      301 ctgttcggat agccgcgcac cagtttggaa aagtcactaa acgcagctcg tgcatgttga
      361 ggatcgcgat cgctacggtc aacgccaaag aacccttgca gcgcactgtc atccagcgcc
      421 atattggtca ggccacgcat gtacatgaca taatcgatat tcggatgggt cgggttaagg
      481 cgaataaaac gatcgatggc agcctgtgct aacggcaaat cggcgttttt atagtaggcg
      541 tagatgagat ccagctgcac ctgctgcgaa tacggaccaa acggatagcg attatctaac
      601 gcttccagtt gcgttattgc ctgtctccag ttaccgtcct gcagcttttg ttgtgcagtc
      661 gcgtaaattt catttggcgg attatcaggt acttcttcct ttgaccccga gcaacccgcc
      721 aaaaacaggc ttagtgtggc ggctgccacc agatatttca tgcgcgtcat gacgttttga
      781 ctttcctcaa aatgtaatac gggagattct ctgttcctgc tcccggttaa gaccagctac
      841 aatagcacac tatattaaac ggcaaagccg taaaacccca acgataaacg aagaagcagt
      901 atatatggca caacgagtac agctcactgc aacggtgtcc gaaaaccaac tcggtcaacg
      961 cttagatcag gctttggccg aaatgttccc ggattattca cgttcgcgga taaaagaatg
     1021 gatcctcgac cagcgcgtgc tggttaacgg caaagtttgt gataagccga aagaaaaagt
     1081 attgggtggc gagcaggttg ccatcaacgc tgagattgaa gaagaagcgc gttttgaacc
     1141 gcaggatatc ccgctggata tcgtctatga agatgaagat atcattgtca ttaataaacc
     1201 gcgcgaccta gtagtacatc ctggcgcggg taacccggat ggcacggtac tgaatgcgtt
     1261 gcttcattat tatccgccca ttgccgatgt accgcgtgcg ggcatcgtcc atcgtctgga
     1321 taaagacacc accggcctga tggttgtggc aaaaaccgtt ccggctcaga cgcgtttagt
     1381 cgaatctttg caacggcgtg aaattactcg tgagtatgaa gcggtggcga ttggtcatat
     1441 gaccgctggc ggtacagtag atgaacccat cagccgccac ccgaccaaac gtactcacat
     1501 ggcggtgcat ccgatgggca aaccggcggt gactcactat cgcatcatgg aacacttccg
     1561 tgtgcacacg cgtctgcggt tgcgtctgga aactggacgt acgcaccaga tccgcgtgca
     1621 tatggcccat atcactcatc cgctggtggg cgatccggtt tatggtggcc gtccgcgtcc
     1681 gccaaaaggt gcttcggaag catttatctc cacgctgcgt aagtttgacc gccaggcgct
     1741 tcatgcaacc atgctgcgtc tttatcaccc gatctctggc atcgaaatgg aatggcatgc
     1801 gcctattcca caagatatgg tggagctgat tgaggtgatg cgcgccgatt tcgaagagca
     1861 taaggatgaa gtggactggt tatgagtaag ctgattgtcc cgcagtggcc actgccaaaa
     1921 ggtgttgcgg cctgtagctc cactcgtatc ggcggcgtga gcttgccccc gtatgactca
     1981 ctcaacctcg gtgcccattg tggcgataac ccggatcacg ttgaggagaa tcgtaagcga
     2041 ctttttgctg cgggcaattt gccttctaaa ccggtctggc ttgagcaggt acacggcaaa
     2101 gatgtgctta agcttactgg cgaaccttat gcctcaaaac gagcagatgc ctcttatagc
     2161 aatacgcctg gcacggtttg cgcagtgatg actgccgact gcctccctgt gctgttttgc
     2221 aatcgagcgg gaacggaagt cgccgccgtt catgctggct ggcgtggact gtgcgcaggc
     2281 gtgctggaag agacggtttc ctgttttgct gataaacctg aaaacattct cgcctggtta
     2341 gggccggcaa ttggtccacg cgcgttcgaa gtgggggcgg aggttcgcga ggcgtttatg
     2401 gcagtagacg ctaaagcaag tgcagctttc attcagcatg gtgataagta tctggcggat
     2461 atttatcagc ttgcccggca gcgtctggcg aacgtgggtg ttgagcaaat tttcggcggc
     2521 gaccgttgta catatacgga aaatgagact ttcttctctt atcgtcgcga caagaccacc
     2581 ggtcgtatgg caagtttcat ttggctgata taacctaaag aatcaagacg atccggtacg
     2641 cgtgattttc ttttcacatt aatctggtca ataaccttga ataattgagg gatgacctca
     2701 tttaatctcc agtagcaact ttgatccgtt atgggaggag ttatgcgtct ggatcgtctt
     2761 actaataaat tccagcttgc tcttgccgat gcccaatcac ttgcactcgg gcacgacaac
     2821 caattcatcg aaccacttca tttaatgagc gccctgctga atcaggaagg gggttcggtt
     2881 agtcctttat taacatccgc tggcataaat gctggccagt tgcgcacaga tatcaatcag
     2941 gcattaaatc gtttaccgca ggttgaaggt actggtggtg atgtccagcc atcacaggat
     3001 ctggtgcgcg ttcttaatct ttgcgacaag ctggcgcaaa aacgaggtga taactttatc
     3061 tcgtcagaac tgttcgttct ggcggcactt gagtctcgcg gcacgctggc cgacatcctg
     3121 aaagcagcag gggcgaccac cgccaacatt actcaagcga ttgaacaaat gcgtggaggt
     3181 gaaagcgtga acgatcaagg tgctgaagac caacgtcagg ctttgaaaaa atataccatc
     3241 gaccttaccg aacgagccga acagggcaaa ctcgatccgg tgattggtcg tgatgaagaa
     3301 attcgccgta ccattcaggt gctgcaacgt cgtactaaaa ataacccggt actgattggt
     3361 gaacccggcg tcggtaaaac tgccatcgtt gaaggtctgg cgcagcgtat tatcaacggc
     3421 gaagtgccgg aaggattgaa aggccgccgc gtactggcgc tggatatggg cgcgctggtg
     3481 gctggggcga aatatcgcgg tgagtttgaa gaacgtttaa aaggcgtgct taacgatctt
     3541 gccaaacagg aaggcaacgt catcctgttt atcgacgaat tacataccat ggtcggcgcg
     3601 ggtaaagccg atggcgcaat ggacgccgga aacatgctga aaccggcgct ggcgcgtggt
     3661 gaattgcact gcgtaggtgc cacgacgctt gacgaatatc gccagtacat tgaaaaagat
     3721 gctgcgctgg aacgtcgttt ccagaaagtg tttgttgccg agccttctgt tgaagatacc
     3781 attgcgattc tgcgtggcct gaaagaacgt tacgaattgc accaccatgt gcaaattact
     3841 gacccggcaa ttgttgcagc ggcgacgttg tctcatcgct acattgctga ccgtcagctg
     3901 ccggataaag ccatcgacct gatcgatgaa gcagcatcca gcattcgtat gcagattgac
     3961 tcaaaaccag aagaactcga ccgactcgat cgtcgtatca tccagctcaa actggaacaa
     4021 caggcgttaa tgaaagagtc tgatgaagcc agtaaaaaac gcttggatat gctcaacgaa
     4081 gaactgagcg acaaagaacg tcagtactcc gagttagaag aagagtggaa agcagagaag
     4141 gcatcgcttt ctggtacgca gaccattaaa gcggaactgg aacaggcgaa aatcgctatt
     4201 gaacaggctc gccgtgtggg ggacctggcg cggatgtctg aactgcaata cggcaaaatc
     4261 ccggaactgg aaaagcaact ggaagccgca acgcagctcg aaggcaaaac tatgcgtctg
     4321 ttgcgtaata aagtgaccga cgccgaaatt gctgaagtgc tggcgcgttg gacggggatt
     4381 ccggtttctc gcatgatgga aagcgagcgc gaaaaactgc tgcgtatgga gcaagaactg
     4441 caccatcgcg tcattggtca gaacgaagcg gttgatgcgg tatctaacgc tattcgccgt
     4501 agccgtgcgg ggttggcgga tccaaatcgc ccgattggtt cattcctgtt cctcggccca
     4561 acgggtgtgg ggaaaactga gctttgtaag gcgctggcga actttatgtt tgatagcgac
     4621 gaggcgatgg tccgtatcga tatgtccgag tttatggaga aacactcggt gtctcgtctg
     4681 gttggtgcgc ctccgggata tgtcggttat gaagaaggtg gctacctgac cgaagctgtg
     4741 cgtcgtcgtc cgtattccgt catcctgctg gatgaagtgg aaaaagcgca tccggatgtc
     4801 ttcaacattc tgttgcaggt actggatgat ggtcgtctga ctgacgggca ggggagaacg
     4861 gtcgacttcc gtaatacggt tgtcattatg acctctaacc tcggttccga tttgattcag
     4921 gaacgcttcg gtgaactgga ttatgcgcac atgaaagagc tggtgctcgg tgtggtaagc
     4981 cataacttcc gtccggaatt cattaaccgt attgatgaag tggtggtctt ccatccgctg
     5041 ggtgaacagc acattgcctc gattgcgcag attcagttga aacgtctgta caaacgtctg
     5101 gaagaacgtg gttatgaaat ccacatttct gacgaggcgc tgaaactgct gagcgagaac
     5161 ggttacgatc cagtctatgg tgcacgtcct ttgaaacgtg ctatccagca gcagatcgaa
     5221 aacccgctgg cacagcaaat actgtctggt gaattggttc cgggtaaagt gattcgcctg
     5281 gaagttaatg aagaccggat tgtcgctgtc cagtaatgat aaaacgagcc cttcggggct
     5341 cgtttttgtc tataagttag acggaaaaga ctatatttaa gatgttttgc ctgaaaaatg
     5401 agcgaacgat aaagttttta tatttttcgc ttgtcaggcc ggaataactc cctataatgc
     5461 gccaccactg acacggaaca acggcaaaca cgccgccggg tcggcggggt tctcctgaga
     5521 attccggcag agaaagcaaa aataaatgct tgactctgta gcgggaaagc gtattatgca
     5581 caccccgcgc cgctgagaaa aagcgaagcg gcactgctct ttaacaattt atcagacaat
//
LOCUS       DADMKF010000205         5620 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.209, whole genome shotgun sequence.
ACCESSION   DADMKF010000205 DADMKF010000000
VERSION     DADMKF010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5620
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.209"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(<1..513)
                     /locus_tag="JNC24_003758"
     CDS             complement(<1..513)
                     /locus_tag="JNC24_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HAY9160405.1"
                     /translation="MGKYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSVGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSA
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIIDAELFALAGLDA
                     QHLQLLVHSGS"
     gene            complement(759..1211)
                     /locus_tag="JNC24_003759"
     CDS             complement(759..1211)
                     /locus_tag="JNC24_003759"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAY9160406.1"
                     /translation="MNNIQIRNYQPGDFQQLCAIFIRAVTMTASQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVWVAVINAQPVGFISRIEHYIDMLFVDPEYTRRGVASALLKPFIK
                     SESELTVDASITAKPFFERYGFQTVKQQHVECRGAWFTNFYMQYKPQH"
     gene            complement(1208..2263)
                     /gene="dinB"
                     /locus_tag="JNC24_003760"
     CDS             complement(1208..2263)
                     /gene="dinB"
                     /locus_tag="JNC24_003760"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HAY9160407.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVMLLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            complement(2334..3119)
                     /gene="lafU"
                     /locus_tag="JNC24_003761"
     CDS             complement(2334..3119)
                     /gene="lafU"
                     /locus_tag="JNC24_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HAY9160408.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSITTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLDNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDSKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRTR"
     gene            3064..4803
                     /gene="flhA"
                     /locus_tag="JNC24_003762"
     CDS             3064..4803
                     /gene="flhA"
                     /locus_tag="JNC24_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhA"
                     /protein_id="HAY9160409.1"
                     /translation="MLSRSDLLTLLTINFIVVTKGAERISEVSARFTLDAMPGKQMAI
                     DADLNAGLINQAQAQTRRKDVASEADFYGAMDGASKFVRGDAIAGMMILAINLIGGVC
                     IGIFKYNLSADAAFQQYVLMTIGDGLVAQIPSLLLSTAAAIIVTRVSDNGDIAHDVRN
                     QLLASPSVLYTATGIMFVLAVVPGMPHLPFLLFSALLGFTGWRMSKRPQAAEAEEKSL
                     ETLTRTITETSEQQVSWETIPLIEPISLSLGYKLVALVDKAQGNPLTQRIRGVRQVIS
                     DGNGVLLPEIRIRENFRLKPSQYAIFINGIKADEADIPADKLMALPSSETYGEIDGVL
                     GNDPAYGMPVTWIQPAQKAKALNMGYQVIDSASVIATHVNKIVRSYIPDLFNYDDITQ
                     LHNRLSSMAPRLAEDLSAVLNYSQLLKVYRALLTEGVSLRDIVTIATVLVASSAVTKD
                     HILLAADVRLALRRSITHPFVRKQELTVYTLNNELENLLTNLVNQAQQGGKVMLDSVP
                     VDPNMLNQFQSTMPQVKEQMKAAGKDPVLLVPPQLRPLLARYARLFAPGLHVLSYNEV
                     PDELELKIMGALM"
     gene            complement(4926..5423)
                     /gene="rayT"
                     /locus_tag="JNC24_003763"
     CDS             complement(4926..5423)
                     /gene="rayT"
                     /locus_tag="JNC24_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase RayT"
                     /protein_id="HAY9160410.1"
                     /translation="MSEYRRYYIKGGTWFFTVNLRNRRSQLLTTQYQMLRNAIIKVKR
                     DRPFEINAWVVLPEHMHCIWTLPEGDDDFSSRWREIKKQFTHACGLKNIWQPRFWEHA
                     IRNTKDYRHHVDYIYINPVKHGWVKQVSDWPFSTFHRDVARGLYPIDWAGDVTDINAG
                     ERIIL"
     gene            complement(5494..>5620)
                     /locus_tag="JNC24_003764"
     CDS             complement(5494..>5620)
                     /locus_tag="JNC24_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY9160411.1"
                     /translation="HIAINILTNDKVFKAGLRRKMRKAAMDRNYLASVLEGSGLS"
BASE COUNT         1339 a         1468 c         1460 g         1353 t
ORIGIN      
        1 cgagccgcta tgaaccaaca gttgcagatg ttgtgcatcc agacctgcca gtgcaaacag
       61 ttcagcgtcg atgatttgat caacttgttg cagttcagcg aaatgattgc cgccaccgat
      121 agagccaagc gagctgcgcc acggatgctg tgcaaatgct gacggcaggt tttcctccag
      181 ccagctttct tcagcaacgt catccaaagc tgataatcgc ttttcaaact tatcggcgtt
      241 gtatttgcga gcgagaatat ctgtttgcca tagcgccatg ccgcagccga tatcattgcc
      301 gaccagtgct gggtaaaaac ggccaacaga gaagaacgct gcgccaatcg ggtagccgcg
      361 tccgggatgt aaatctggca tccccactac gcgctgcatg ttgggtaaat ttgccgtggt
      421 gtgtaattgt tggatcgcta aactctcgat ccacaggtca tcagatgcga tggtaaatac
      481 cgcatcggat aagggacgaa tatatttgcc catgtaccat tccgtaaata gactcgtcat
      541 acttcaagtt gcatgtgctg cgactcgaat tattgagagt aacaattaaa aaatcaggaa
      601 atggcagacg aatccagggc aagaaaaaaa gaattaatcc tggaaaggtc tgcaaagagt
      661 cattgataaa ggatttgtca tgatttacct ccttgcagtt tgtggaataa gaaaacgcgc
      721 acatactagg cgggcgttat tttcattgca agctggattt aatgttgcgg tttatattgc
      781 atataaaaat tagtaaacca cgctccccgg cattcaacgt gctgctgctt aactgtctga
      841 aaaccataac gttcaaaaaa gggttttgcg gttatgctgg catctaccgt aagttcggat
      901 tcagacttaa taaatggttt taacaaggcg ctggcaaccc cgcggcgggt gtattcaggg
      961 tcaacaaata acatatcgat ataatgttca atgcgggaaa taaaaccaac cggttgtgca
     1021 ttaatgaccg caacccacac ttgtgatttc gcgagtttct ccttccagcg agattcgtca
     1081 atctgcgccc aggcggcaat ttgttgtgga gaataatgct gactggcggt catcgtaacc
     1141 gctctaatga aaatagcgca tagttgctga aaatcgccag gctgatagtt tcttatttgt
     1201 atgttattca taatcccagc accagttgtc tttccatttg cggatcaagc aacgtcacat
     1261 gcagccccac cagtcgcaca ccgcgcccgc cgcggcgttc atcccaggtt ttacgcgcgg
     1321 tggcgattaa gtcagcttta ttcagccgcg gccagacgtg ctcctgagtg gtttgctgaa
     1381 aatcgtcgaa ctttaatttc accccctggc gggcaatcag taaatcaggt tttacctttg
     1441 ccagacggcg ttcaagttcc ggatacagcc gctcgataat cgcttcacat tcagaccagt
     1501 ggtggatatc ttccgccatc gtgcgttcca cgccgacgga ttttcgcaac cgttcgctgt
     1561 taacgtcgcg ctcgtcaatc ccctgactac gctcccacaa aatgcggcca aatttgccaa
     1621 agcgtttaag cagcatcacc agatcacact tttgtacatc accgcaggtt cgtagcccca
     1681 tcgcttccag ttttgccgcc gagactttgc cgacgccggg gatttttgcc agtggtaagg
     1741 tttgtaaaaa tgccggaact tctgccggcg taatcacaaa ctggccgttg ggtttattca
     1801 tgtcggaggc gattttggcg agaaacttta cgggtgccac gcccgcagac gccgtcagtt
     1861 gcagctcgtt gaaaatcgtc tggcggattt cctgggcgat gagggtcgca gaaccgtggc
     1921 aatggacgct atcggtgacg tcgagataag cctcatccag tgacaacggt tcaatacgcg
     1981 aggtgtagcg cgagaagatt tcgcggatat gatttgaggc ttctttgtag gcgtcaaagc
     2041 gccccggaag caaggtgaga tgcgggcata atttgagcgc catccctgtc ggcatagcgc
     2101 tacgtacgcc aaatttacgc gcgggataat tggcggtact gatcaccccc cgacgttcgc
     2161 ggctgccgcc aatagcaata gggatatcgc gcagggcggg attgtcgcgc atctccaccg
     2221 ctgcgaaaaa gcagtccata tccacatgaa tgattttacg cattgctcac ctctcaacac
     2281 tggtaaagta tacagtgatt tcagagtttg agaaatgcgt aaagattcag catttaacgc
     2341 gttcgctgcg aaagcacctg ctgcttatcc agcttttgca ccagcggctg caccacttta
     2401 tccccatgct gaccaaagta ttgatacagc gtatcggacg cacttttggt cagcaccata
     2461 atctcaatgc gccggttgcc cgcgctttgc ggatttttgg aatccagcag catctggtcc
     2521 gccattgcgc ttacctgcat cactttatct tccggcattc cggcctcttc cagcacccga
     2581 cgagccgaaa gcgcgcgatc gcccgaaagg ttccagttgt tgtagatatt gtttttgtaa
     2641 gccatcgcat cggtatgccc ggtaataata attttgttat cgagcgagtc gaaaactggc
     2701 gcgagctcca ccagcagcgt tttaaagaac ggcataatct gcgcgctgcc gcgttcgaac
     2761 atattgcgat tctggtcgtc tttaatcagc acgcgtaatc cctggggaac aatctccatc
     2821 tccagattcg cttccatatg cgcatcacgg gcaatggtgg tgatactggt tgccagctcg
     2881 ccaagctcgg tggcggattt cttcttcagc agcgccgttt tctcattgac gtcgcgcgct
     2941 tttttctccg tttcttccgg caccgccacg gtggcaggtt ttggcggatg agacgggctg
     3001 attttattta acggcgacag cccgccaccg ttaaaaatcg actgcccgtg cagcgcggcg
     3061 ataatgcttt cacgctcgga tttactgacg ctgttgacaa tcaactttat tgtcgtcacc
     3121 aaaggggccg agcgtatctc cgaggtttct gcccgcttta ccctggacgc gatgcccggt
     3181 aagcagatgg cgattgacgc cgatcttaat gccgggttga tcaatcaggc gcaggcacaa
     3241 acccgacgta aagatgttgc cagcgaggcc gatttctacg gcgcgatgga cggggcatcg
     3301 aaatttgtgc gcggggatgc tatcgccggg atgatgattc tggcgatcaa cctgatcggc
     3361 ggcgtctgta tcgggatctt caaatacaac ctgagcgccg acgccgcctt ccagcagtat
     3421 gtgctgatga ccatcggcga cgggctggtg gcgcaaatcc cttccctgct actctccacc
     3481 gcggcggcga ttatcgtcac ccgcgtcagc gacaacggcg atatcgccca tgacgtgcgt
     3541 aaccagttgc tggcaagccc atcggtgctc tacaccgcta ccggaattat gttcgtactg
     3601 gcggtagtgc cgggaatgcc acatctgccg tttttgctgt tcagcgccct gcttggcttt
     3661 accggctggc ggatgagcaa acgcccgcag gcggcggaag cggaagagaa aagtctcgaa
     3721 acgctgaccc gcaccatcac cgaaaccagc gagcaacagg tcagttggga aaccattccg
     3781 ctgatcgagc ccatcagctt aagcctcggc tacaagctgg tggcgctggt ggacaaagcc
     3841 cagggcaacc cgctcaccca gcgtattcgc ggcgtgcgac aggtgatatc cgacggcaac
     3901 ggcgtgctgc tgccggagat ccgcattcgg gaaaacttcc gccttaagcc cagccagtac
     3961 gctattttca tcaacggcat taaggctgat gaagcggata ttccggcgga taaactgatg
     4021 gcgctgccct ccagcgaaac ctacggcgag atagacggcg tgctggggaa cgacccggcg
     4081 tacgggatgc cggtgacctg gatccagcct gcgcagaaag cgaaggcgct gaatatgggg
     4141 tatcaggtga tcgacagcgc cagcgtgatt gctacccatg taaataagat tgtgcgcagc
     4201 tatattcctg atttatttaa ctatgacgat attacgcagt tgcataaccg tttgtcgtcg
     4261 atggcaccgc gcctggcgga ggatttaagc gcggtgctca attacagcca gttgctgaaa
     4321 gtgtaccggg cgctgctgac cgaaggcgtt tccctgcgcg atatcgtcac catcgccacc
     4381 gtgctggtcg ccagtagcgc ggtgaccaaa gatcatattc tgctggcagc cgatgtgcgc
     4441 ctggcgctgc ggcgcagcat tacccatccg ttcgttcgca agcaggagct gacggtgtat
     4501 accctgaata atgagctgga aaatctgctg actaacctag tgaatcaggc gcaacagggc
     4561 ggtaaagtga tgctcgacag cgtgccagtg gacccgaata tgctcaacca gttccagagc
     4621 acgatgccac aggtgaaaga gcagatgaaa gcggcgggga aagacccggt gctgctggtg
     4681 ccaccgcagc tgcgcccttt gctggcgcgt tatgcaaggt tgtttgcgcc ggggctgcat
     4741 gtgctgtcgt ataacgaagt gccggacgag ctggagttga agattatggg ggcgttgatg
     4801 taagtatccg gcattggtgc acaactgccg gatgcggcgt aaacgcctta tccggcctac
     4861 cgttcaggca caagtttgta ggcatgataa gacgcgccag cgtcgcatca ggcatctgcg
     4921 cataattata aaatgatacg ctcgccagca tttatatccg ttacgtcccc cgcccaatcg
     4981 atgggatata accctcgcgc gacatcgcga tggaacgttg agaatggcca atcactcact
     5041 tgctttaccc aaccatgctt tactggattt atataaatat aatcaacatg atgcctataa
     5101 tctttggtat tgcggatggc gtgctcccaa aaacgtggtt gccagatatt tttcaatcca
     5161 caagcatggg taaattgctt tttaatttcc cgccagcgag aggaaaaatc atcatcgcct
     5221 tcaggtaatg tccatatgca atgcatatgc tctggcaaaa cgacccaggc gttgatttca
     5281 aaaggcctgt ctcgcttaac tttaataatg gcgttacgga gcatctggta ctgggtggtc
     5341 aagagttggc ttcgacgatt gcgtaaattc accgtgaaaa accaggttcc ccccttgatg
     5401 taataacgac gatattcaga catggcgact cctttttctc aacggtaaga agaaaaggaa
     5461 aggtcggcaa tgaggtgatg gaacagggca agattacgaa agcccgctcc cctcaaggac
     5521 tgacgccaga tagtttctgt ccatggctgc ttttcgcatc ttacgtctta accctgcctt
     5581 gaatacctta tcattcgtca aaatattaat agcgatgtgc
//
LOCUS       DADMKF010000206         5614 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.210, whole genome shotgun sequence.
ACCESSION   DADMKF010000206 DADMKF010000000
VERSION     DADMKF010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5614
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.210"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            12..428
                     /gene="soxR"
                     /locus_tag="JNC24_003765"
     CDS             12..428
                     /gene="soxR"
                     /locus_tag="JNC24_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001129347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HAY9160412.1"
                     /translation="MAKRSGVAVSALHFYESKGLITSIRNSGNQRRYKRDVLRYVAII
                     KIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREELDRRIHTLVALRDELDG
                     CIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            974..2323
                     /locus_tag="JNC24_003766"
     CDS             974..2323
                     /locus_tag="JNC24_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAY9160413.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            2475..4124
                     /locus_tag="JNC24_003767"
     CDS             2475..4124
                     /locus_tag="JNC24_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HAY9160414.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(4278..5569)
                     /locus_tag="JNC24_003768"
                     /pseudo
     CDS             complement(4278..5569)
                     /locus_tag="JNC24_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
BASE COUNT         1256 a         1270 c         1456 g         1632 t
ORIGIN      
        1 cccccggcga agtggcgaaa cgcagcggtg tggcggtatc ggcgctgcat ttctatgaaa
       61 gtaaagggtt gattaccagt atccgtaaca gcggcaatca gcggcgatat aaacgtgatg
      121 tgttgcgata tgttgcaatt atcaaaattg ctcagcgtat tggcattccg ctggcgacca
      181 ttggtgaagc gtttggcgtg ttgcccgaag ggcatacgtt aagtgcgaaa gagtggaaac
      241 agctttcgtc ccaatggcga gaagagttgg atcggcgcat tcatacctta gtggcgctgc
      301 gtgacgaact ggacggatgt attggttgtg gctgcctttc gcgcagtgat tgcccgttgc
      361 gtaacccggg cgaccgctta ggagaagaag gtaccggcgc acgcttgctg gaagatgaac
      421 aaaactaaag cgccacaagg gcgctttagt ttgttttccg gtctttgtct ttctctctat
      481 cccgctggta cacaggaggg tttcccccga cgtcaacaca ccccattcga gcacgtggtg
      541 gaggttccgg ttggtgttga tgctttaatt gtatgtcacc ggcatttctt cgccagtgta
      601 aaagtatact ttttatccgc aatatttttg tcatctcaga cgatttttta tcgcaatcct
      661 gaacggtata cggctcgata acgctgcaat cttgcgcacc gacgataacg tttgcgcatc
      721 aattgcctgg tttttcatcg tcaagacaat aaaagagaaa aaagcagcaa acttcggttg
      781 aaaaagccgc tatgatcgcc ggataatcgt ttgctttttt taccacccgt tttgtatgcg
      841 cggagctaaa cgtttgcttt tttgcgacgc agtaaattgt cgcaaacctg gagcaggaag
      901 ataacgtttc tctggcaggg gattgtccgc cacgcatctt gacgaaaatt aaactctcag
      961 gggatgtttt cttatgtcta cgccatcagc gcgtaccggc ggttcactcg acgcctggtt
     1021 taaaatttca caacgtggaa gcactgtccg tcaggaagtg gttgccgggt taacaacgtt
     1081 tctggcgatg gtctactcgg tcatcgtcgt tccaggtatg ttgggtaaag caggcttccc
     1141 gcctgcggca gttttcgttg caacctgtct ggttgccgga ctcggttcta tcgtgatggg
     1201 tctgtgggct aatctgccgt tggcgattgg ttgcgccatc tccctgacgg cgtttaccgc
     1261 attcagcctg gtgctggggc aacatattag cgtacctgtc gcgctgggtg ccgtgttcct
     1321 gatgggtgtg ctgtttacgg taatttctgc cacgggtatc cgtagctgga ttttgcgcaa
     1381 cttgccgcac ggtgtggcgc acggcacggg gatcggtatc ggtctgttcc tgctgctcat
     1441 tgccgctaat ggtgtcggtc tggtgattaa aaacccgctt gatggtctgc ccgttgcgct
     1501 gggtgatttc gcgaccttcc cggtgattat gtcactggta ggtctggcgg tgatcatcgg
     1561 cctggaaaaa ctgaaagtcc ctggtggcat tctgctgacc attatcggta tctcaattgt
     1621 cggtttgatc ttcgatccta acgtccattt ctccggcgtt ttcgccatgc cttcattgag
     1681 cgatgaaaac ggcaattcac tgattggcag cctggacatt atgggcgcgc tgaatcctgt
     1741 agtcctgcca agcgttctgg cgctggtgat gacggcagta tttgatgcca ccggaactat
     1801 ccgtgccgtc gccggccagg cgaacctgct ggataaagat gggcagatca tcgacggtgg
     1861 gaaagcactg accactgact ccatgagcag cgttttctct ggcctggtgg gtgctgctcc
     1921 ggcagcggta tacatcgagt ctgcggcggg tacggcggcg ggcggtaaaa ccggtttgac
     1981 ggctatcacc gttggcgtgc tgttcctgct gattctgttc ctctctccgc tctcttacct
     2041 cgttccgggg tatgcaacgg ctccggcgct gatgtacgtt ggcctgctga tgctgagcaa
     2101 cgtggcgaaa atcgactttg ctgattttgt tgatgcgatg gcgggtctgg ttacggcggt
     2161 attcatcgtg ctgacctgta acatcgtaac aggcatcatg atcggcttcg cgactctggt
     2221 gattggtcgt ctggtttccg gcgaatggcg caagttgaac atcggtacgg tcgttatcgc
     2281 cgtggcgctg gtgaccttct atgcgggtgg ctgggctatc taatctttca tcggattctg
     2341 aaaacgggtg gcaatggctg cccgttttta ttttctccgc acattgtgtg agcttttgcg
     2401 atattctgaa aaaatgagaa ttcaggcata acgcctttct cagatcataa aaaaacatcg
     2461 caaacaggga acgcatggaa atcttcttca ccatactgat aatgaccctc gtggtctcac
     2521 tgtccggggt ggtcactcgt gtcatgccct ttcagatccc gcttccgctt atgcaaatcg
     2581 ccatcggtgc gctactggcg tggccgacgt ttggtttgca tgtggagttt gatcctgaac
     2641 tctttttagt cttgtttatc ccgccgttgc tgttcgctga tggctggaaa acgccgaccc
     2701 gtgaatttct cgaacatggt agagagattt tcggcctcgc gctggcgctg gtggtggtca
     2761 ccgtggtcgg cattggcttc cttatttact gggtggtgcc gggcattccg ctgatccccg
     2821 cctttgcgct ggcggcggta ctttctccga ccgatgctgt ggcgctctcc gggattgttg
     2881 gcgaagggcg catcccgaaa aaaatcatgg gcattttgca gggcgaagcg ttgatgaatg
     2941 acgcctccgg cctggtgtcg ttgaagtttg ccgtggcagt ggcgatgggg acgatgatct
     3001 tcaccgtcgg cggtgcgacg gtcgaattta tgaaagtagc cattggtggt attcttgccg
     3061 gttttgtggt gagctggctg tatggtcgct cgctgcgatt cctcagccgc tggggcggtg
     3121 atgaacccgc gacgcagatc gttctgctgt tcttgctgcc attcgcttct tatctgattg
     3181 ccgaacatat tggcgtttcc ggcatcctcg ctgcggttgc cgccgggatg accatcaccc
     3241 gctccggtgt gatgcgccgt gcgccgctgg caatgcgcct gcgcgcaaac agcacctggg
     3301 cgatgctgga atttgtcttt aacggcatgg tgttcctgct gttaggtctg cagctgccgg
     3361 gtattctgga gacgtcgctg atggcggcag aaatcgaccc taacgtcgaa atctggatgc
     3421 tgtttaccga tattattctg atatatgcgg cgctgatgct ggtccgtttc ggctggctgt
     3481 ggacgatgaa aaagttcagc aaccgcttcc tgaagaagaa gccgatggag tttggttcgt
     3541 ggaccacacg agaaatcctg atcgcgtctt tcgccggggt gcgtggggcg atcactctgg
     3601 ccggtgtgct ctctatcccg ctgctcttgc cggatggtaa cgtcttcccg gcgcgctatg
     3661 agctggtgtt cctggcggct ggcgtcattc tcttctcgct gtttgtcggc gtggtgatgt
     3721 tgcctattct gctacaacac attgaagtcg cggaccattc gcaacaattg aaagaagaac
     3781 gtattgcgcg agcggcaacg gcagaagtgg cgattgtggc gatccagaaa atggaggagc
     3841 gtctggcggc ggataccgaa gagaatatcg ataaccagct gcttacggag gtcagttctc
     3901 gcgtcattgg taacctgcgt cgtcgcgccg atggacgtaa cgacgttgaa agttccgtgc
     3961 aggaagagaa ccttgagcgt cgcttccgtc tggcggcatt gcgttctgaa cgtgctgaac
     4021 tgtaccacct gcgcgccacg cgggagatca gcaacgaaac gctgcaaaaa ttactgcacg
     4081 atctcgattt gcttgaagcg ttgctaattg aggaaaatca gtaagccgaa agcgacgtaa
     4141 ccaggcccga ggtaatctga aaaaggtgca atcttttgca cctttaacag gttagctcgg
     4201 gctaacattc atgattctaa aacaaataag ttatctctct tgtaaagtca attcattggc
     4261 tgttcttcga cttaacctca atctgcgtgt tgcataatat ctgcagtaat cttcatagcg
     4321 actgcattaa tattatcctc agtttgtgac atgccagtat gagaggtaca atcttcttca
     4381 aagaatttct gtagtgttac cagggtatca ctatcaatgg ggatattttc ctccccacgg
     4441 agtatggaaa tgcccattcc ctggacgcta gcctcatcaa aattaacccg atagagacgg
     4501 gtattaaaca tacaggcttt tgttaaaata gaacagttga aatggcagtt gcttaaattg
     4561 gcattattaa aattaacatt agccatatta gagtcggtac atataaccat ttcgaggaat
     4621 gtatttgtta gccacgtaca aaagagtttg ctatcaatta agtttatatt ctggaggatt
     4681 gctttgttca gattgacgcg agacagatca gcatgttcaa agttaattaa atcgagttta
     4741 gagaaactaa ggtcggcttt aaataaatta acatatggcg ctattacttt ataaagattt
     4801 gcctcagcca tgtacgccat ataaaaattt gaatagtcaa gaaaggtatt tttaatattg
     4861 gctctattca ttgctgcatt gtagaattga acatgcataa cggagcatgc atttagagtt
     4921 ccgtcggcca tttttatctg agcgaaattt gagtcatcga gaatacaatt atcaaaaagt
     4981 atttttccat tatggcgtaa taaaaattcg ttttatacat attacagtcc tggaaaatag
     5041 cgttttgtag tctgcaacct gcaaaattcg aattggataa atcttcatct ttaatcacga
     5101 tacctttcag gctaaggtgt gaaaaatcgt aaccaccatt tactgtctgt ggaatttcga
     5161 tagtattatt atggacggcg acattattta taaaatatac caccttaatg gatacttcct
     5221 tttctttact aaggtaaact cttctcgtaa ttctatcgtc acatttatta tttgcatata
     5281 aagctatatc tataaaatgt ttatgtattt tcctgtccgt ttccttcgtg atttttcctg
     5341 atggtaaact atcataaatt cttttcattt tctgaatcaa acctatcact ttttcatgat
     5401 catccatctt gaaaagagag agggctttat tgtcaattaa cgtgttttta tgatgtttgc
     5461 tgtaattata tgattttgtt ttctttgtat gtgatgaact tgtgaaagag tggttatcgt
     5521 taatctggca aagagagaaa aacatattat ttaaaccatt atagcgcata aaatatcctt
     5581 ataattaaca agaaagaaaa ggcatattct ctgc
//
LOCUS       DADMKF010000207         5554 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.211, whole genome shotgun sequence.
ACCESSION   DADMKF010000207 DADMKF010000000
VERSION     DADMKF010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5554
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.211"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            298..867
                     /locus_tag="JNC24_003769"
     CDS             298..867
                     /locus_tag="JNC24_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004743146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAY9160415.1"
                     /translation="MRTFLYARVSVDDLTTQNQVEAVKRAGYEVRPSRVIEEVISGAT
                     PAMDRPQFVKLVDKLEEGDCLVVTKIDRLGRDNIDVQKTVTMLLGMGVKLVCLDLPVQ
                     DLSSAEGKLILQLFSTFAEFELNRIRERTREGLERAKAQGKVLGRPVAKNTTEAVLEH
                     KAKGLSQSQVAKLLNLSVRTVSRHWTKTL"
     gene            1596..1769
                     /locus_tag="JNC24_003770"
     CDS             1596..1769
                     /locus_tag="JNC24_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HAY9160416.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGQHSGGNFKNDPQRASEAG
                     KKGGKSSHGKSDN"
     gene            complement(2027..3355)
                     /gene="rutG"
                     /locus_tag="JNC24_003771"
     CDS             complement(2027..3355)
                     /gene="rutG"
                     /locus_tag="JNC24_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HAY9160417.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPDERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSHAAWFGLPHFSTPAFNSQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNGNLIMVAVTL
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQEP"
     gene            complement(3376..3870)
                     /gene="rutF"
                     /locus_tag="JNC24_003772"
     CDS             complement(3376..3870)
                     /gene="rutF"
                     /locus_tag="JNC24_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization flavin reductase protein
                     F"
                     /protein_id="HAY9160418.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPSGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHTTPYGLVWFDRSYHALM
                     RPAC"
     gene            complement(3881..4471)
                     /locus_tag="JNC24_003773"
     CDS             complement(3881..4471)
                     /locus_tag="JNC24_003773"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HAY9160419.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLRELYAPIKWGP
                     TSANCSPARIVFIRTVEGKERLHPALSSGNLQKTLTAPVTAIVAWDSEFYERLPLLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQHVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            complement(4481..5281)
                     /gene="rutD"
                     /locus_tag="JNC24_003774"
     CDS             complement(4481..5281)
                     /gene="rutD"
                     /locus_tag="JNC24_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HAY9160420.1"
                     /translation="MKLSLSPPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIEHYAVVGHALGALVGMQLALDYP
                     ASVTVLISVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPSACSS
                     ELHAALPDSQKMVMRYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            complement(5289..>5554)
                     /locus_tag="JNC24_003775"
     CDS             complement(5289..>5554)
                     /locus_tag="JNC24_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyrimidine utilization protein C"
                     /protein_id="HAY9160421.1"
                     /translation="VLFADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWKN
                     YAAINEIYAEFFPGDKPARFCIQCGLVKPDALVEIATIAHIAK"
BASE COUNT         1466 a         1456 c         1385 g         1247 t
ORIGIN      
        1 attccccttc taaaaatctt cgaaaatatc tgacagatgt ctgtagctgc taagtacccg
       61 tcacaccagc tatgtgaccc cctttgcaaa aaagccaaat atcattgact ttctcggtga
      121 attgctataa aggcatctat ctaggtcttt aactgtataa atacacatga caacaaacgc
      181 ccgttaagtg tctatataaa aaggtagctg tagctataga caacaattgt gataattgat
      241 ttaatggcta tagacaagca agacagacct ttgttgacta tatagagaga taagaacatg
      301 cgtacattcc tttatgctcg tgtatcagtt gatgacctca ccacacagaa ccaagtagaa
      361 gctgttaaac gtgctggtta tgaagtcaga ccttcacgag taattgagga agttattagt
      421 ggggctacac cagctatgga cagaccacaa tttgtaaagc tggtggataa gctggaagaa
      481 ggtgattgtc ttgtagtgac caagattgac cgcttaggaa gggataacat tgacgtacag
      541 aagaccgtca caatgttact tggtatgggt gttaagctgg tctgtctaga tttgcctgtc
      601 caagaccttt caagtgctga aggtaaactt attcttcaac tattcagtac ctttgcagag
      661 tttgaactta accgcatcag agagcgaacc agagagggct tagaacgagc taaagcacaa
      721 ggtaaggtgt tgggtcgtcc tgtagctaaa aacactacag aggccgtttt agagcataag
      781 gctaagggat tgtcacaaag tcaggttgca aagcttctga atctctctgt gagaacggtt
      841 agccgtcact ggacaaagac actgtaaaaa aaacttagta gcatcttcat agcctccttc
      901 atggggactt ttttgtgtct gtaacatatg gtgaccaaac atctatatat cgttatatag
      961 ccctgtatat agcactatat atagtgtctg gagagggacg actaacaccc tgcatagttc
     1021 cctgaaaagg gggcttcaca gattctctat atatttcaat aagttaggat aaaaagggac
     1081 tataccgata ctgcatagaa tctgtgtagg attgcatagt cataggggtt ggaacaagcc
     1141 caactacacc tgataaagcc tgttattaac aggtagttga atgtacgtgt ttagctatgt
     1201 aaaagcacca gaactttagc catttctaac cactcctcgt gttatctcta ttccgtagcg
     1261 attcgctacc acttatttaa agataagacg tccttttcag agtgcaaatt tcacaaccac
     1321 ttatttgatt tataacaact ttcacaagca cgtaattttg tcgcaaaatg acacattttt
     1381 atctcatcgc gtttttttaa tcataagagc ggcttatgga taattattgg agatgatatc
     1441 tattctcgct aagaagctgt tgcaggatat taccaaacgc gggtctaccc gcgtcagttc
     1501 actaagctta gtcccacgta gcgaaaatat ggcagccgcc atacgccgcg ttaattctat
     1561 gcaatatgat gtctataccc agacggaggt cagtaatggc aaaccatcga ggcggttccg
     1621 gcaattttgc agaagaccgc gaaagagcat cagaagcagg taaaaaaggt ggacagcaca
     1681 gcgggggtaa tttcaagaat gacccgcagc gcgcatctga agcaggtaaa aaaggtggca
     1741 agagcagtca cggcaaaagc gacaactagc cgggctaatc aatgacgaat gcatttttgt
     1801 ctgtagctcg tcaaaaagcc atcaccgccg gttacccggt ggttgatact gatgacaaat
     1861 gtaagcttgc ctgatgcgcg atgcttatca ggcctaccag aagattgcaa tatattgaat
     1921 ttgcactgtt ttgtaggccg gataaggcgt ttacgccgca tccggcatga acaatgcgta
     1981 cgttgtcaac aatctgcacc gccgggtaac cccggcggtt ttctgtttat ggctcctgat
     2041 gaacaacttc tggcggtgga acgtcaacca accttctact taacaacgca ttgagtaaaa
     2101 tcgcgccaaa ggtggctgta ccgatccctc ccaacgtaaa accgcccagc gtgagagcaa
     2161 aatcacccgc gcccagcact aaggttactg cgaccataat caaattaccg ttctggctta
     2221 aatcgacacg gttttgtacc cagatccttg cgcctgcgac ggcaatcagc ccgaacacaa
     2281 caattgatgc accaccaatc accgcggccg gaatggtatg gatcagcgcg ccaaatttcg
     2341 gcgaaaagcc caacagcatg gcgatgacgg cagcagcaac aaacaccagc gtcgagtaga
     2401 cttttgtcac ggccatcaca ccgatatttt cagcataggt ggtgacgccg ctaccgccga
     2461 cagagccgga aagcatcgtt gccagaccat caccgacgaa tgctcgcccc atatacgggt
     2521 ccatattgcg tccggtcatc ccggcgaccg ccttgagatg acctaagttt tccgccacca
     2581 gaatcaccgc cacgggcgca atcagcatca ttgcctgact attaaaagca ggagtggaaa
     2641 aatgtggcaa accgaaccag gcagcatggc tgacgagagt aaaatcgacg gcttttccca
     2701 gccctaaaac gttggtcatc acgccataca gcagacaggc gacaattaaa cctacgagaa
     2761 tcaataaccg ctggatcatg ccacgggtaa acaccgccac cagcccaata cacagcaccg
     2821 tcattaccgc catccagcta tcaaaggctg aagccgatac acttttcact gcgataggcg
     2881 ctaagttcag gccaatcgcc atcaccaccg cacccgtcac caccggcggc attagtcgtt
     2941 caatccagcg tgtaccaatt ttcatcacca ccaggccaat gacggtataa accagcccac
     3001 aggcgataat cccgcccagt gcgatgctga tattcgggtt aatgccctga ccgttaaagc
     3061 ccgtcgcggc aatcaccacg ccgacaaaag ccgcgctgga gccgagataa ctggggacgc
     3121 gcccgccggt aataaagaaa aacagtaacg tgccgatccc cgacattaaa atggaaagat
     3181 tgggatccag ccccatcaga atcggcatta acaccgtcgc gccaaacatc gccaccgcgt
     3241 gttgaacgcc cattactgcc gtctgagcaa acggcaatcg ttcatccggc gcgaccacgc
     3301 cgctctctgt agaggtcgat tttaactgcc agtgaggaaa accgaacatt gccatcagct
     3361 gtctccttaa ggaggttaac aagcagggcg catcagcgcg tgataactgc gatcgaacca
     3421 caccagcccg tagggtgtgg tgtgacgatg aatcgcttcg atggcgcaaa acagaatgtc
     3481 gtgggtgccg acgctcacca cctggctgat gcggcagtca aacgaaacca gagcctcttc
     3541 cagttgcggg catccggtca cacccgtctg ccagcgggcg gcggcaaagc ggtgttccat
     3601 gggcgttttg ccgccaaaaa gatttgaaag cggctcctgc ccggcgctca gtgtatttac
     3661 acacagcgtt cgattttcat tgaataccgg ccagacggac gccccacgat tcaggcacac
     3721 cagtaatgtg ggcggcgtgt cggtcacgct gcagacggcg ctggcggtga acccggcgcg
     3781 cccggatgga ccgtccgtgg tgatgatatt gaccgccgcg cccatacagg acatcgcatc
     3841 gcgaaaggtt tgttgatcaa ctatgttcat agtttgctcc ttacaacagc ccgcaggctt
     3901 cttcaaagga cagacgtggc aggcgcgcaa aaagtttgct gctatcgcca tagccgatat
     3961 taatcagcag attgctcttc agcgtgctgc ccgcaaaaaa ggcgtcgtca acgtgttgac
     4021 ggtcaaaacc cgacatcggg ccggtatcca gtcccagcgc acggcaagcg acgatcagat
     4081 aggccgcctg catggagctg ttgcgaaacg ctgtttcttc ggcaagttgt gggctggagg
     4141 taaaccaact gcgggcatca ccgtggggaa acagtagtgg taaccgttca taaaattcac
     4201 tgtcccaggc aacgatagcg gtgacgggcg cggtcagggt tttttgtaga ttgccgctgg
     4261 aaagcgccgg gtgcagacgt tcttttcctt ctacagtgcg gataaacacg atccgtgccg
     4321 gagaacagtt agctgatgtc ggcccccatt ttatcggggc ataaagctcc cgtaacgtct
     4381 catcgctgac gggtgtctcc cgccagccgt tgtgagtgcg ggcatcggtg aacagggtgc
     4441 taagcgcacc tgggctaacg gcttcgttca tagcaattcc ttacagggcg gcttcacggt
     4501 gatgtaacag gctggcaagc ccgttgagta acagagcatt aaacgtttcg gggtcggtca
     4561 cgttgcaggc gtgtccgcca tagcgcatca ccattttctg gctatcgggc agggcggcat
     4621 gaagctcact ggaacatgct gatggcacca gcagatcatc actggcgcag atgatttgca
     4681 ccgggcagcg gatgcgatcc gcatggtgac taaagtcagc gcgtttgagg gcgttaagtc
     4741 gacgcagtaa attatttttg ccctgaaaat gcgccagtgc cagcgcgtct tctgcctcaa
     4801 ggcgaggtgc gcgggccgcc atccagtcgg cgggatagag gaacaacggc tgcgcttcca
     4861 cccatgcctg cgcgccgccg ctatacagta atcgttcgcg aacctgaaaa cagcggcgcg
     4921 tatgggcgtt tattcgtagc cagccgttaa cgctgatcag cacagttacc gacgcgggat
     4981 aatccagcgc cagctgcatt cccaccagcg caccgagcgc atggccgacc accgcgtaat
     5041 gctcaatccc tgcggctacc agcgcctgat gcagttccgc tgccatctgg gcgatactgt
     5101 aatcttctgc cagcgtgtcg ggattattgc cggtgccgcg ctggtcgtaa cagactacct
     5161 gatactcctg ctccagcacc gccagttgcg gtaaccagta actgccgcta ccgccaagac
     5221 ccgaaatcaa caccactacg ggcgcatcag cataaggggg aggtgagagt gaaagtttca
     5281 tcgcggcctc acttggcgat atgcgcaatt gtggcgattt ccaccagcgc gtcaggtttt
     5341 accagtccgc actgaatgca gaatcgcgcc ggtttatcac ccggaaaaaa ctcggcgtag
     5401 atttcgttaa tcgcggcgta atttttccag tcggtaataa agatgctgtt gaaggtcaca
     5461 tccgccatcg tgccacccgc cgtctcgatc accttgcgga tagtttccag aacgtggcgg
     5521 gtttgcgcct ttgggtcatc ggcaaacagc acgt
//
LOCUS       DADMKF010000208         5378 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.212, whole genome shotgun sequence.
ACCESSION   DADMKF010000208 DADMKF010000000
VERSION     DADMKF010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5378
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.212"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(55..1338)
                     /gene="aroA"
                     /locus_tag="JNC24_003776"
     CDS             complement(55..1338)
                     /gene="aroA"
                     /locus_tag="JNC24_003776"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HAY9160422.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGVSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLGSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
     gene            complement(1409..2497)
                     /gene="serC"
                     /locus_tag="JNC24_003777"
     CDS             complement(1409..2497)
                     /gene="serC"
                     /locus_tag="JNC24_003777"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HAY9160423.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            complement(2696..3388)
                     /locus_tag="JNC24_003778"
     CDS             complement(2696..3388)
                     /locus_tag="JNC24_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HAY9160424.1"
                     /translation="MKAFDLHRMAFDKVPFDFLGEVALRSLYTFVLVFLFLKMTGRRG
                     VRQMSLFEVLIILTLGSAAGDVAFYDDVPMVPVLIVFITLALLYRLVMWLMAHSEKLE
                     DLLEGKPVVIIEDGELAWSKLNNSNMTEFEFFMELRLRGVEQLGQVRLAILETNGQIS
                     VYFFEDDKVKPGLLILPSDCTQRYKVVPESADYACIRCSEIIHMKAGEKQLCPRCANP
                     EWTKASRAKRVT"
     gene            3518..5278
                     /locus_tag="JNC24_003779"
     CDS             3518..5278
                     /locus_tag="JNC24_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory protein YcaO"
                     /protein_id="HAY9160425.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLTENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLEAEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
BASE COUNT         1378 a         1386 c         1269 g         1345 t
ORIGIN      
        1 atgtgccgac gttttgataa agaaagattt ggctatttat tgcccgttgt tcattcaggc
       61 tgcctggcta atccgcgcca actgctcgaa ataatccgga aatgttttgg ccgtacattt
      121 gggatcaaga atcgtcactg gtgtatctga caacgccacc agcgagaaac acatcgccat
      181 ccggtgatca ttgtatgtcg cgatctcggc aaagttcagt ttttccggtg gagtgatacg
      241 aatgtaatcg tgcccctctt ctacttccgc accgacttta cgcagttctg ttgccatcgc
      301 aaacaggcga tcggtctctt taacacgcca gttatagata ttgcgcagcg tggtggtgcc
      361 ttttgcaaat aacgccgccg tggcaatggt catcgccgca tcgggaatat ggttcatatc
      421 catatcaata gcgttcagtt caccacgcgt gcaggaaata taatcatcgc cccagcaaat
      481 ggtcgcgccc attttttcca gcacatcagc aaagcgaata tcaccctgca tactgttacg
      541 tccaataccg gtcactttta cagtgccgcc tttgattgct gctgctgcca gaaagtaaga
      601 agccgaagat gcatcgcctt cgaccaaata agtacccgga gactgataag actgcccgcc
      661 ttttacgaca aattgttgat agtgctgatt ttcaatttca acaccaaacg tcttcatcag
      721 attgagtgtg atgtcgatat aaggtttaga aaccagatcg cctttaatac gaatcaccgt
      781 atcttccggc gcaagaggcg cagtcattaa cagtgcggtg aggaattggc tggaaacgga
      841 gccatcaacg tcaacgttgc cgccagtaaa gccgccctgt aaacgcaacg gcggataatt
      901 ttcttgttcc aggtaagtga tcttcgcccc gcccaggcgc agcgcatcca ccagatgacc
      961 aatcgggcgt tctttcatac gcggctcacc ggtcagcaca atatcattgc tacccagaca
     1021 aagagctgcc gccagcggac gcattgccgt tccggcgtta ccgaggaaca actccagggc
     1081 accttctgcg tgtaatggac cgccgttacc gataatttcg caacgcgtac gatcggctga
     1141 aagcgtatag cttaccccta acgctgttaa tgcattcagc atatggcgca cgtcatcgct
     1201 atccagcaga ttggttaata ctgttttgcc gtgtgctaat gccgccagca ataaagcgcg
     1261 gttagaaacg ctcttggaac cgggcagatt aatagtgcca tcgacacgag cgatgggttg
     1321 taacgtcagg gattccatga actcaactct ctacaacaga aataaaaacc ccacaggctg
     1381 gctgtgggga ttaagcaaaa tttcggcatt aaccgtgacg gcgttcgaac tcaaccatga
     1441 agtctgtcag cgctttaacg ccttccagcg gcatggcgtt ataaatagaa gcgcgcattc
     1501 cgccgaccac acggtgacct ttcagtgcat gaaggccagc agcaaaagac tcttcaagga
     1561 acaatttgtc aagcgcactg tccgccaact ggaacggcac gttcatccgc gaacggttag
     1621 ctttcgccac gtcattgcgg tagaaatcgc tgttatcaat caccccatat agcagttctg
     1681 ctttttgctg attgatttta tccatttcag ctacaccgcc gttcgctttc agccatttaa
     1741 agaccagacc agatagatac caggcaaatg tcggcggcgt gttaaacatg gagccgttat
     1801 cgttgaggat ggaataatcc agaatcgacg gacacgcgat attcgctttg cccagcaaat
     1861 cttcacgaac gatgacgatt gtcaggccag ccgggccgat atttttctgc gcgccagcgt
     1921 aaatcacgcc ataacggctg acgtcaatcg gacgggaaag aatggttgaa gagaagtcgg
     1981 cggcgaccac cacatctttg ccgaagtctg gcgtttcgtc gatggcgata ccgtcgatgg
     2041 tttcattcgg gcagtaatgc atataagcag cattatcaga gagttgccat tcacgcattg
     2101 gcttaaccgc gcgcagacca tcaacagtca ctttggcgtc aaagacatta ggcgtgcagt
     2161 attttttcgc ttctttaatg gcacttgccg cccagtaacc ggcatcaaca taatctgcgg
     2221 tggttttatc accgagaata ttcagcggta ccgcagcaaa ctgaccgcga ccaccgccat
     2281 ggcagaataa taccttgtag ttggagggga cattaagaag atcgcgaaaa tccttctcgg
     2341 cttcctctgc aacctgaatg aactctttgc cacggtgact cacttccatc accgacgtac
     2401 caagaccgtt ccagtcgcgc agttcctgtt gagcctgttt aagcacctct gccggtagca
     2461 ttgccggacc agaactaaaa ttgaagattt gagccattgc ccctcaccac gttgcgttgc
     2521 tattaacccg cggtcaatcg attgcgacca caggtaatac attattcctg tggctatcgg
     2581 ttttatcatt cagtgacaca catcgcaatg agtaaaaccg ttgagggcaa aaatgtggct
     2641 tgcatcacac aaaagaatct ctcggattat tagcaaaaag ccgatgtttt tactgtcagg
     2701 tcacccgttt tgcccgactt gccttcgtcc attctggatt tgcacagcgc ggacataatt
     2761 gtttttcccc cgctttcata tgaatgattt cactacaacg aatacaggca tagtccgccg
     2821 actccggcac cactttgtaa cgctgcgtac aatcactggg taaaataagt aaacccggtt
     2881 tcaccttgtc atcttcaaag aaatagacac tgatttgccc gttggtttcg agaatcgcca
     2941 gacgtacctg ccccagctgc tccacgccac gcaatcgtag ctccataaag aactcaaatt
     3001 ccgtcatgtt ggagttattg agtttcgacc aggccagctc gccatcttca ataataacaa
     3061 ccggcttacc ttccagtaga tcttccagtt tttcactgtg cgccatcaac cacattacca
     3121 ggcggtataa caacgccaga gtgataaaga cgataagtac cggcaccatc ggcacatcat
     3181 cataaaacgc cacatctccc gccgctgaac ccagcgtgag aatgattaac acttcaaaca
     3241 gcgacatctg tcgcacaccg cgtcttccgg tcattttgag gaacaaaaaa accagtacaa
     3301 aggtataaag actacgtagt gcaacttcgc caaggaaatc aaaaggcact ttatcaaatg
     3361 ccatacggtg gagatcgaac gccttcattt ttatgctctt atcgtggttt atccgctaaa
     3421 agcatagtca acagttcatc ggcatggcgg cggtgatagc gcctttgaga taaaacccgt
     3481 attattgcgc gctttccgta cgactaaagt gattttcatg acgcaaacat ttatccccgg
     3541 caaagatgcc gctctggaag attccatcgc tcgcttccag caaaaacttt cagacctcgg
     3601 ctttcagatt gaagaggcct cctggctgaa tcctgtgcct aacgtctggt ctgtacatat
     3661 tcgcgacaaa gagtgcgcac tgtgttttac caacggtaaa ggcgcaacca aaaaagcggc
     3721 gctggcttct gcacttggtg aatatttcga gcgtctctca accaactact tttttgctga
     3781 tttctggctg ggcgaaacca tcgccaacgg tccgttcgtg cattatccca acgaaaaatg
     3841 gttcccactg accgaaaatg acgatgtgcc agaaggacta ctcgatgacc gtctgcgcgc
     3901 gttttatgat ccggagaatg aactgaccgg cagcatgctg attgacctac aatccggtaa
     3961 cgaagatcgt ggtatttgcg gcctgccgtt tacgcgtcag tccgacaatc agaccgttta
     4021 tattccgatg aatatcattg gtaacctgta cgtctccaac ggtatgtccg caggtaatac
     4081 ccgcaacgaa gcacgcgttc agggattgtc tgaagttttc gaacgctacg tgaaaaaccg
     4141 cattattgct gaaagcatca gcctgccaga gatcccggca gacgtgctgg cgcgttaccc
     4201 agcagtagtt gaagccatcg aaacgctgga agcggagggt ttcccaatct tcgcttatga
     4261 cggttcgctt ggcggccagt atccggtgat ttgcgtggta ctgttcaatc ctgctaacgg
     4321 tacctgcttt gcctctttcg gtgcgcatcc tgattttggc gtagcactgg aacgtaccgt
     4381 gaccgagctg ctgcaaggtc gtggcctgaa agatttggat gtgtttactc cgccaacctt
     4441 cgatgatgaa gaagtcgctg aacataccaa cctcgaaacg cactttatcg attccagcgg
     4501 tttaatctcc tgggacctgt tcaagcagga tgccgattat ccgtttgtgg actggaattt
     4561 ctccggcacc acggaagaag agtttgctac gctgatggct atcttcaaca aagaagataa
     4621 agaagtttat attgccgatt acgagcatct gggcgtttat gcttgccgta ttatcgtgcc
     4681 tggcatgtcc gatatttatc cggctgaaga tctgtggctc gcgaataaca gtatgggcag
     4741 ccatttacgt gaaacgattc tttcgctacc aggcagcgag tgggaaaaag aagattacct
     4801 gaacctcatc gagcaactgg atgaagaagg ttttgatgac tttacccgcg tgcgtgagct
     4861 gttgggtctg gcgaccgggt cggataacgg ttggtacacc ctgcgtatcg gtgaattaaa
     4921 agccatgctg gcgctggctg gtggcgatct ggaacaggct ctggtctgga ccgaatggac
     4981 gatggagttt aactcatcag tatttagtcc ggaacgcgcc aactattatc gctgcctgca
     5041 aacgttgtta ttactggcac aggaagaaga tcgccagccg ctgcaatatc tgaatgcgtt
     5101 tgttcgcatg tacggcgcag atgccgtaga agccgccagt gcggcaatga gcggcgaagc
     5161 ggcgttttac ggcctgcaac cggtagatag cgatctgcac gcgtttgctg cacatcagtc
     5221 gttgttgaag gcctacgaaa agctgcagcg cgccaaagca gcattctggg caaaataaaa
     5281 tcaaatagcc tacgcaatgt aggcttaatg attagtctga gttatattac ggggcgtttt
     5341 tttaatgccc cgctttacat atatttgcat taataaaa
//
LOCUS       DADMKF010000209         5323 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.213, whole genome shotgun sequence.
ACCESSION   DADMKF010000209 DADMKF010000000
VERSION     DADMKF010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5323
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.213"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            168..1025
                     /gene="focA"
                     /locus_tag="JNC24_003780"
     CDS             168..1025
                     /gene="focA"
                     /locus_tag="JNC24_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HAY9160426.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
     gene            1080..3362
                     /gene="pflB"
                     /locus_tag="JNC24_003781"
     CDS             1080..3362
                     /gene="pflB"
                     /locus_tag="JNC24_003781"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HAY9160427.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKQLEKIVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKLAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDVLNYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            3554..4294
                     /gene="pflA"
                     /locus_tag="JNC24_003782"
     CDS             3554..4294
                     /gene="pflA"
                     /locus_tag="JNC24_003782"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HAY9160428.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(4387..4563)
                     /locus_tag="JNC24_003783"
     CDS             complement(4387..4563)
                     /locus_tag="JNC24_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005043118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H oxidoreductase"
                     /protein_id="HAY9160429.1"
                     /translation="MQSERIYLVWAHPRHDSLTAHIADAIHQRAMERKIQVTELDLYR
                     RNIISRYYLRYGIW"
     gene            4663..>5323
                     /locus_tag="JNC24_003784"
     CDS             4663..>5323
                     /locus_tag="JNC24_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY9160430.1"
                     /translation="MRMNMSDFATFFAVARNQSFRAAGDELGLSSSAISHSIKTLEQR
                     LKIRLFNRTTRSVSLTEAGSNLYERLRPAFDEIQIMLDEMNDFRLTPTGTLKINAARV
                     AARIFLMPLLVGFTREYPDIKVELTTDDSLVDIVQQGFDAGVRLSGIVEKDMISVAIG
                     PPVKLCVAATPEYFARYGKPRHPHDLLNHQCVVFRYPSGKPFHWQFAKEQEIAVAGNI
                     IL"
BASE COUNT         1368 a         1308 c         1277 g         1370 t
ORIGIN      
        1 tttttaaaat ttatttttat ttggataatc aaatgtttac tccgtatctg cataaaaacc
       61 atgcgagtta cgggcctata agccaggcga gatatgatct atatcaattt ctcatctata
      121 atgctttgtt agtatctcgt cgccgactta ataaagagag agttagtgtg aaagctgaca
      181 acccttttga tcttttactt cctgctgcaa tggccaaagt ggccgaagag gcgggtgtct
      241 ataaagcaac gaaacatccg cttaagactt tctatctggc gattaccgcc ggtgttttca
      301 tctcaatcgc attcgtcttc tatatcacag caaccactgg cacaggcaca atgcccttcg
      361 gcatggcaaa actggttggc ggcatttgct tctctctggg gctgattctt tgtgttgtct
      421 gcggagccga tctctttact tccaccgtgt tgattgttgt tgctaaggcg agtgggcgca
      481 tcacctgggg tcagttggcg aaaaactggc taaatgtcta ttttggcaac ctggtcggcg
      541 cactgctgtt tgtactttta atgtggcttt ccggcgagta tatgaccgca aatggtcaat
      601 ggggactaaa cgtcctacaa accgccgacc acaaagtgca ccatactttt attgaggccg
      661 tctgtcttgg tatcctggca aacctgatgg tatgtctggc agtatggatg agttattctg
      721 gccgcagcct gatggacaaa gcgttcatta tggtgctgcc ggtcgcgatg tttgttgcca
      781 gcggttttga gcacagtatc gcaaacatgt ttatgatccc gatgggtatt gtaatccgcg
      841 acttcgcatc cccggaattt tggaccgcag tcggttctgc accggaaaat ttttctcacc
      901 tgaccgtgat gaatttcatc actgataacc tgattccggt tacgatcggc aacattatcg
      961 gtggtggttt gttggttggg ttgacatact gggtcattta cctgcgtgaa aacgaccacc
     1021 attaatggtt gtcgaagtac gcagtaaata aaaaatccac ttaagaaggt aggtgttaca
     1081 tgtccgagct taatgaaaag ttagccacag cctgggaagg ttttaccaaa ggtgactggc
     1141 agaatgaagt aaacgtccgt gacttcattc agaaaaacta cactccgtac gagggtgacg
     1201 agtccttcct ggctggcgct actgaagcga ccaccaccct gtgggacaaa gtaatggaag
     1261 gcgttaaact ggaaaaccgc actcacgcgc cagttgactt tgacaccgct gttgcttcca
     1321 ccatcacctc tcacgacgct ggctacatca acaagcagct tgagaaaatc gttggtctgc
     1381 agactgaagc tccgctgaaa cgtgctctta tcccgttcgg tggtatcaaa atgatcgaag
     1441 gttcctgcaa agcgtacaac cgcgaactgg atccgatgat caaaaaaatc ttcactgaat
     1501 accgtaaaac tcacaaccag ggcgtgttcg acgtttacac tccggacatc ctgcgttgcc
     1561 gtaaatctgg tgttctgacc ggtctgccag atgcatatgg ccgtggccgt atcatcggtg
     1621 actaccgtcg cgttgcgctg tacggtatcg actacctgat gaaagacaaa ctggcacagt
     1681 tcacttctct gcaggctgat ctggaaaacg gcgtaaactt ggaacagact atccgtctgc
     1741 gcgaagaaat cgctgaacag caccgcgctc tgggtcagat gaaagaaatg gctgcgaaat
     1801 acggctacga catctctggt ccggctacca acgctcagga agctatccag tggacttact
     1861 tcggctacct ggctgctgtt aagtctcaga acggtgctgc aatgtccttc ggtcgtacct
     1921 ccaccttcct ggatgtgtac atcgaacgtg acctgaaagc tggcaagatc accgaacaag
     1981 aagcgcagga aatggttgac cacctggtca tgaaactgcg tatggttcgc ttcctgcgta
     2041 ctccggaata cgatgaactg ttctctggcg acccgatctg ggcaaccgaa tctatcggtg
     2101 gtatgggcct cgacggtcgt accctggtta ccaaaaacag cttccgtttc ctgaacaccc
     2161 tgtacaccat gggtccgtct ccggaaccga acatgaccat tctgtggtct gaaaaactgc
     2221 cgctgaactt caagaaattc gccgctaaag tgtccatcga cacctcttct ctgcagtatg
     2281 agaacgatga cctgatgcgt ccggacttca acaacgatga ctacgctatt gcttgctgcg
     2341 taagcccgat gatcgttggt aaacaaatgc agttcttcgg tgcgcgtgca aacctggcga
     2401 aaaccatgct gtacgcaatc aacggcggcg ttgacgaaaa actgaaaatg caggttggtc
     2461 cgaagtctga accgatcaaa ggcgatgtcc tgaactatga tgaagtgatg gagcgcatgg
     2521 atcacttcat ggactggctg gctaaacagt acatcactgc actaaacatc atccactaca
     2581 tgcacgacaa gtacagctac gaagcctctc tgatggcgct gcacgaccgt gacgttatcc
     2641 gcaccatggc gtgtggtatc gctggtctgt ccgttgctgc tgactccctg tctgcaatca
     2701 aatatgcgaa agttaaaccg attcgtgacg aagacggtct ggctatcgac ttcgaaatcg
     2761 aaggcgaata cccgcagttt ggtaacaacg atccgcgtgt agatgacctg gctgttgacc
     2821 tggtagaacg tttcatgaag aaaattcaga aactgcacac ctaccgtgac gctatcccga
     2881 ctcagtctgt tctgaccatc acttctaacg ttgtgtatgg taagaaaacg ggtaacaccc
     2941 cagacggtcg tcgtgctggc gcgccgttcg gaccgggtgc taacccgatg cacggtcgtg
     3001 accagaaagg tgcagtagcc tctctgactt ccgttgctaa actgccgttt gcttacgcta
     3061 aagatggtat ctcctacacc ttctctatcg ttccgaacgc actgggtaaa gacgacgaag
     3121 ttcgtaagac caacctggct ggtctgatgg atggttactt ccaccacgaa gcatccatcg
     3181 aaggtggtca gcacctgaac gttaacgtga tgaaccgtga aatgctgctc gacgcgatgg
     3241 aaaacccgga aaaatatccg cagctgacca tccgtgtatc tggctacgca gtacgtttca
     3301 actcgctgac taaagaacag cagcaggacg ttattactcg taccttcact caatctatgt
     3361 aattagattt gactgaaatc gtacagtaaa aagcgtacaa taaaggctcc acgaaagtgg
     3421 ggcctttttt agcacgagag ccttttttgt cagctatcta tactctaagg tgactgccaa
     3481 aacagactcg acgtagcctt cgagctgcgc accaacacgg cctcagatgg gccacatctg
     3541 gagaaacacc gcaatgtcag ttattggtcg cattcactcc tttgaatcct gtggaaccgt
     3601 agacggcccg ggtattcgct ttatcacctt tttccagggc tgcctgatgc gctgcctgta
     3661 ttgtcataac cgcgacacct gggatacgca tggcggtaaa gaagttaccg ttgaagattt
     3721 aatgaaagag gtggtgacct atcgccactt tatgaacgct tccggcggcg gcgttaccgc
     3781 atccggcggt gaggcaatcc tacaagctga gtttgttcgt gactggttcc gcgcctgcaa
     3841 aaaagaaggc attcatacct gtctggacac caacggtttt gttcgtcgtt acgatccggt
     3901 gattgatgaa ctgctggaag taaccgacct ggtaatgctc gatctcaaac agatgaacga
     3961 cgagatccac caaaatctgg ttggagtttc caatcaccgc acgctggagt tcgccaaata
     4021 tctggcgaac aaaaatgtga aggtgtggat ccgctacgtt gttgtcccag gctggtctga
     4081 cgatgacgat tcagcgcatc gcctcggtga atttacccgt gatatgggca acgttgagaa
     4141 aatcgagctt ctcccctacc acgagctggg caaacacaaa tgggtggcaa tgggtgaaga
     4201 gtacaaactc gacggtgtta aaccaccgaa gaaagagacc atggaacgcg tgaaaggcat
     4261 tcttgagcag tacggtcata aggtaatgtt ctaatttccc cgtaaagcgg caactttatt
     4321 gagttgccgc ttttttattc cgcacttcat tctgctacag cgtttttaac acatcttata
     4381 gtgcatctac catatcccgt acctgagata ataacgactg ataatattac gccgatataa
     4441 atcgagttcc gtcacctgta ttttccgctc cattgcccgc tgatggatcg catcagcaat
     4501 atgtgcggtc aatgaatcat gacgaggatg ggcccatacc aaataaatac gttcagactg
     4561 cataatttta cctccacgtt aagttctgga gcgcacttta cgcaggcggc ataataatgt
     4621 aaacgtgatg attcaggatg agttaatgaa ggattttcac taatgcggat gaatatgtct
     4681 gactttgcca ctttctttgc tgtggcccgt aatcaaagct ttcgtgcagc gggcgatgag
     4741 ttaggcttat cctcgtccgc cattagccat agtattaaaa cactggaaca acgtcttaaa
     4801 attcgcttat tcaatcggac aacccgaagc gtatcgttaa cggaagcggg ttctaatctc
     4861 tatgaacgat tgcgcccagc tttcgacgaa attcagatca tgttagatga gatgaatgat
     4921 tttcgtctga caccaacagg aacgctcaaa atcaatgccg cccgggtagc tgcacgtata
     4981 tttttaatgc ctttactggt tggtttcaca cgtgaatatc ctgatattaa agtcgagctc
     5041 acaactgacg actctcttgt cgatatcgtc caacagggct ttgacgctgg cgttcgactt
     5101 agtggcatcg tcgaaaaaga tatgatcagc gtggcgatcg gcccaccagt gaaattatgt
     5161 gtagcagcaa cgccagagta ctttgcaaga tatggcaaac cccgccatcc tcatgatctg
     5221 cttaatcatc aatgtgttgt atttcgttat cccagcggca aacctttcca ctggcaattt
     5281 gcaaaggagc aggaaattgc tgtcgcaggg aatatcattc tgg
//
LOCUS       DADMKF010000210         5298 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.214, whole genome shotgun sequence.
ACCESSION   DADMKF010000210 DADMKF010000000
VERSION     DADMKF010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5298
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.214"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            368..2653
                     /locus_tag="JNC24_003785"
     CDS             368..2653
                     /locus_tag="JNC24_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase 1 subunit
                     alpha"
                     /protein_id="HAY9160431.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPAIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINNLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDTIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            2887..4017
                     /gene="nrdB"
                     /locus_tag="JNC24_003786"
     CDS             2887..4017
                     /gene="nrdB"
                     /locus_tag="JNC24_003786"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HAY9160432.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            4017..4271
                     /gene="yfaE"
                     /locus_tag="JNC24_003787"
     CDS             4017..4271
                     /gene="yfaE"
                     /locus_tag="JNC24_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HAY9160433.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEM"
     gene            complement(4325..4974)
                     /gene="inaA"
                     /locus_tag="JNC24_003788"
                     /pseudo
     CDS             complement(4325..4974)
                     /gene="inaA"
                     /locus_tag="JNC24_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708127.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
     gene            5153..5298
                     /locus_tag="JNC24_003789"
                     /pseudo
     CDS             5153..5298
                     /locus_tag="JNC24_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
BASE COUNT         1312 a         1416 c         1298 g         1272 t
ORIGIN      
        1 gattatgtga tgactcgtgc ttagatcaat ttttgcaatc attaacaaaa agattaataa
       61 accatctata tcaatttatc caacctattc tgccgttcaa gaaatcgccg aacagttatt
      121 tttaacaaat ttttctcttc ccattgactt tcccggacac cttgtctgac ctaaggtgcg
      181 cgaaagccac tttttccttc ctgagttatc cacaaagtta tgcacttgca agagggtcat
      241 tttcacacta tcttgcagtg aatcccaaac atacccccta tatatagtgt tctaagcagc
      301 ttcccttact acaggtagtc tgcatgaaac tattgcggaa agaattccaa aaacaggtac
      361 gacatacatg aatcagaatc tgctggtgac aaagcgcgac ggtagcacag agcgcatcaa
      421 tctcgacaaa atccatcgcg ttctggattg ggcggcagaa ggactgcata acgtttcgat
      481 ttcccaggtc gagctgcgct cccacattca gttttatgac ggtatcaaga cctctgacat
      541 ccacgaaacc attatcaagg ctgccgcaga cctgatctcc cgtgatgcgc cggattatca
      601 gtatctcgcc gcgcgcctgg cgatcttcca cctgcgtaaa aaagcctacg gccagtttga
      661 gccgcctgcg ctgtacgacc acgtggtgaa aatggtcgag atgggcaaat acgataatca
      721 tctgctggaa gactacacgg aagaagagtt caagcagatg gacacgttta tcgatcacga
      781 ccgtgatatg accttctctt atgctgccgt taagcagctg gaaggcaaat atctggtaca
      841 gaaccgcgtg accggcgaaa tctatgagag cgcccagttc ctttatattc tggttgccgc
      901 gtgcctgttc tcgaactacc cgcgtgaaac gcgcctgcaa tatgtgaagc gtttttacga
      961 cgcggtttcc acatttaaaa tttcgctgcc gacgccaatc atgtccggcg tgcgtacccc
     1021 gactcgtcag ttcagctcct gcgtactgat cgagtgcggt gacagcctgg attccatcaa
     1081 cgccacctcc agcgcgattg ttaaatacgt ttcccagcgt gccgggatcg gcatcaacgc
     1141 cgggcgtatt cgtgcgctgg gtagcccgat tcgcggtggt gaagcgttcc ataccggctg
     1201 cattccgttc tacaaacatt tccagacagc ggtgaaatcc tgctctcagg gcggtgtgcg
     1261 cggcggtgcg gcaacgctgt tctacccgat gtggcatctg gaagtggaaa gcctgctggt
     1321 gttgaaaaac aaccgtggtg tggaaggcaa ccgcgtgcgt catatggact acggggtaca
     1381 aatcaacaaa ctgatgtata cccgtctgct gaaaggtgaa gatatcaccc tgttcagccc
     1441 gtccgacgta ccggggctgt acgacgcgtt cttcgccgat caggaagagt ttgaacgtct
     1501 gtataccaaa tatgagaaag acgacagcat ccgcaagcag cgtgtgaaag ccgttgagct
     1561 gttctcgctg atgatgcagg aacgtgcgtc taccggtcgt atctatattc agaacgttga
     1621 ccactgcaat acccatagcc cgtttgatcc ggccatcgcg ccagtgcgtc agtctaacct
     1681 gtgcctggag atagccctgc cgaccaaacc gctgaacgac gtcaacgacg agaacggtga
     1741 aatcgcgctg tgtacgctgt ctgctttcaa cctgggcgca attaataacc tggatgaact
     1801 ggaagagctg gcaattctgg cggttcgtgc acttgacgcg ctgctggatt atcaggatta
     1861 cccgatcccg gccgccaaac gtggagcgat gggtcgtcgt acgctgggta ttggtgtgat
     1921 caacttcgct tactacctgg cgaagcacgg taaacgctac tccgacggca gcgccaacaa
     1981 cctgacgcat aaaaccttcg aagccattca gtattacctg ctgaaagcct ctaatgagct
     2041 ggcgaaagag caaggcgcgt gcccgtggtt taacgaaacc acttacgcga aagggatcct
     2101 gccgatcgat acctataaga aagatctgga taccatcgct aatgagccgc tgcattacga
     2161 ctgggaagca ttgcgtgaat caatcaaaac gcacggcctg cgtaactcta cgctttctgc
     2221 cctgatgccg tccgagacct cttcgcagat ctctaacgcc actaacggta ttgaaccgcc
     2281 gcgcggttac gtcagcatca aagcgtcgaa agacggtatt ttgcgccagg tggtgccgga
     2341 ctacgagcac ctgcacgacg cctatgagct gctgtgggaa atgccgggta acgatggcta
     2401 cctgcaactg gtaggtatca tgcagaaatt tatcgatcag tcgatctctg ctaacaccaa
     2461 ctacgatccg tcacgtttcc cgtcaggaaa agtgccgatg cagcagttgc tgaaagacct
     2521 gctcaccgcc tacaaattcg gggtcaaaac gctgtattat cagaacaccc gtgacggcgc
     2581 tgaagacgcg caggacgatc tggtaccgtc aatccaggac gatggctgcg aaagcggcgc
     2641 atgtaagatc tgatattgag atgccggatg cggcgtaaac gccttatccg gcctacggct
     2701 cggtttgtag gcctgataag acgcgccagc gtcgcatcag gctccgggtg ccggatgcag
     2761 cgtgaacgcc ttatccggcc tacggctcgg atttgtaggc ctgataagac gcgccagcgt
     2821 cgcatcaggc acaggatgcg gcgtaaaatg ccttatccgg cattaaactc ccaacaggac
     2881 acactcatgg catataccac cttttcacag acgaaaaatg atcagctcaa agaaccgatg
     2941 ttctttggtc agccggtcaa cgtggctcgc tacgatcagc aaaaatatga catcttcgaa
     3001 aagctgatcg aaaagcagct ctctttcttc tggcgtccgg aagaagttga cgtctcccgc
     3061 gaccgtatag attaccaggc gctgccggag cacgaaaaac acatctttat cagcaacctg
     3121 aaatatcaga cgctgctgga ttccattcag ggtcgtagcc cgaacgtggc gctattgccg
     3181 cttatttcta ttccggaact ggaaacctgg gtcgaaacct gggcgttctc agaaacgatt
     3241 cattcccgtt cctatactca tatcattcgt aatatcgtta acgatccgtc tgttgtgttt
     3301 gacgatatcg tcaccaacga gcagatccag aaacgtgcgg aagggatctc cagctattac
     3361 gatgaactga tcgaaatgac cagctactgg caccttctgg gcgaaggcac ccacaccgtt
     3421 aacggtaaaa ccgtaaccgt tagcctgcgc gagctgaaga aaaaactgta tctctgcctg
     3481 atgagcgtta acgcgctgga agcgatccgc ttctacgtca gctttgcttg ttcctttgcc
     3541 tttgcagaac gcgaactgat ggaaggtaac gccaagatta ttcgcctgat tgcccgcgat
     3601 gaagccctgc acctgaccgg tactcagcat atgctgaatc tgctgcgcag cggcgcggat
     3661 gatccggaga tggcggaaat tgccgaagaa tgtaagcagg agtgctatga cctgtttgtc
     3721 caggcggcac agcaagagaa agactgggcg gattatctgt tccgtgacgg ttcgatgatt
     3781 ggtctgaata aagatattct ctgccagtac gttgaataca tcaccaatat ccgcatgcag
     3841 gcagtcggtt tggatctgcc gttccagacg cgctccaacc cgatcccgtg gatcaacacc
     3901 tggctggtgt ctgataacgt gcaggttgct ccgcaggaag tggaagtcag ttcttatctg
     3961 gtcgggcaga ttgacgcgga agtggacacc gacgatttga gtaacttcca gctctgatgg
     4021 cccgcgttac cctgcgcatc actggcacac aactgctgtg ccaggatgaa cacccttccc
     4081 ttctggcggc gctggaatcc cacaatgttg cggttgagta ccagtgccgc gaaggttact
     4141 gcggctcctg tcgcacccga ctggtcgcag gtcaggtgga ctggattgcc gaaccgttag
     4201 cctttattca gccgggggaa attttgccct gttgttgccg ggcaaaaggc gatattgaaa
     4261 tcgagatgtg aattggttgt agtgccagat acaacgctta tacgtcttat ctggcctaca
     4321 acgattacat ggcgtagtaa tacgctttca cctgctccca gtccgctttg gggattggct
     4381 ccagatattt tccagctggc ggaagtcatg attaatcgct ttattgcgac gcaagcggcg
     4441 acgacttttc tccaggtcaa gaaaaccagc ttctgcttta ccttctgttt tcacatagat
     4501 atggcgaaca taacaacagc catgctgacg attaacgcta tgcattttct taaacgccag
     4561 cgccactgct ttcaacatgg cttgccgtac ttcgtcagaa tagggcgata ctgcatgccg
     4621 ggcataccag tcagcaatgc tgatgaaccc cgccatatct tcagtcacca gcaacgcgcg
     4681 ccattcaccc tcaattttca ccgcttcacc aaaaacgatc ttcggcacga tgacaccggc
     4741 ccgttccagt tctttaatta ccgcaacctc acggacaatc gttggtcggc cgaacggata
     4801 acgtacggaa tgaaacagat gatgcgtcat gcgctttaca tacagctttt tgccgttgcg
     4861 ctcgacgcat tgcaccccgc tcataccatt acggcgatag ttaggctctt caacccagtc
     4921 gccctctgtt gcccaccagt gattaaattc gtcgtacttt gctgaaactg ccatacccta
     4981 tcgcctgtca tttttattaa cgacaatgac tataggtggt tacctgagga aaatcttaat
     5041 gaaacgtgtc gtattaatga atttcttttg ccgctactag tgtgacaccg cttttgccct
     5101 tcatttgcag cgcatagatt ccaccagcca cgaccactaa tagtgcgccc agcagataaa
     5161 atgtgaattt aatcaggtgg atacagcgat gaattttatt cgacagggat taggcatcgc
     5221 cttattacca gagttaacgc tgaaaaccat tgcaggtgaa ttgtgttccg ttcctcacga
     5281 accaaccttc tatcgaca
//
LOCUS       DADMKF010000211         5290 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.215, whole genome shotgun sequence.
ACCESSION   DADMKF010000211 DADMKF010000000
VERSION     DADMKF010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5290
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.215"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            313..399
                     /gene="leuL"
                     /locus_tag="JNC24_003790"
     CDS             313..399
                     /gene="leuL"
                     /locus_tag="JNC24_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HAY9160434.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            492..2063
                     /gene="leuA"
                     /locus_tag="JNC24_003791"
     CDS             492..2063
                     /gene="leuA"
                     /locus_tag="JNC24_003791"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HAY9160435.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSSAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKAQHNENNKETV"
     gene            2063..3154
                     /gene="leuB"
                     /locus_tag="JNC24_003792"
     CDS             2063..3154
                     /gene="leuB"
                     /locus_tag="JNC24_003792"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HAY9160436.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLNAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKRF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAACAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            3157..4557
                     /gene="leuC"
                     /locus_tag="JNC24_003793"
     CDS             3157..4557
                     /gene="leuC"
                     /locus_tag="JNC24_003793"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HAY9160437.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFNGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKTGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVKRASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            4568..5173
                     /gene="leuD"
                     /locus_tag="JNC24_003794"
     CDS             4568..5173
                     /gene="leuD"
                     /locus_tag="JNC24_003794"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HAY9160438.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIAAYEAKQPAFMN"
     gene            complement(5222..>5290)
                     /locus_tag="JNC24_003795"
     CDS             complement(5222..>5290)
                     /locus_tag="JNC24_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY9160439.1"
                     /translation="VYWVIAVISVVALFLTAKVKDV"
BASE COUNT         1349 a         1401 c         1396 g         1144 t
ORIGIN      
        1 caatatcaaa aaaaatcgca aaacatataa ttcaatacaa atcatcagga taggttttgc
       61 aacgcgtgca ttttgtcccc tttttcctcg ttgattagat gcaaaaattt atgctgaaat
      121 atgtcaaccg atgaaaagcg tcggtagtta agcagaaatt aatatcgctt actttaacca
      181 ccgcagcaca attagctaat tttacggatg cagaactcac gctggcggga cgtttttatt
      241 gcgtcagggt tgacatccgt ttttgtatcc agtaactcta aaagcatatc gcattcatct
      301 ggagctgatt taatgactca catcgttcgc tttatcggtc tactactact aaacgcatct
      361 tctttgcgcg gtagacgagt gagcggcatc cagcattaag ccagcacgca gtcaaacaaa
      421 aaacccgcgc cattgcgcgg gtttttttat gcccgaagcg aggcgctcta aaagagacaa
      481 ggacctaaac catgagccag caagtcatta ttttcgatac cacattgcgc gacggtgaac
      541 aggcgttaca ggcaagcttg agtgtgaaag aaaaactgca aattgcgctg gcccttgagc
      601 gtatgggtgt tgacgtgatg gaagtcggtt tccccgtctc ttcgccgggc gattttgaat
      661 cggtgcaaac catcgcccgc caggttaaaa acagccgcgt atgtgcgtta gctcgctgcg
      721 tggaaaaaga tatcgacgtg gcggccgaat ccctgaaagt cgccgaagcc ttccgtattc
      781 atacctttat tgctacctcc ccaatgcaca tcgccaccaa gctgcgcagc accctggacg
      841 aggtgatcga acgcgctatc tatatggtga aacgcgcccg taattacacc gatgatgttg
      901 aattttcttg cgaagatgcc gggcgtacac ccattgccga tctggcgcga gtggtcgaag
      961 cggcgattaa tgccggtgcc accaccatca acattccgga caccgtgggc tacaccatgc
     1021 cgtttgagtt cgccggaatc atcagcggcc tgtatgaacg cgtacctaac atcgacaaag
     1081 ccattatttc cgtacatacc cacgacgatt taggcctggc ggtcggcaac tcactggcgg
     1141 cggtacatgc cggtgcacgc caggtggaag gtgcaatgaa cgggatcggc gagcgtgccg
     1201 gtaactgttc gctggaagaa gtcatcatgg cgattaaagt tcgtaaggat attctcaacg
     1261 tccacaccgc cattaatcac caggagatat ggcgcaccag ccagttagtt agccagattt
     1321 gtaatatgcc gatcccggca aacaaagcca ttgttggcag cagcgcattc gctcactcct
     1381 ccggtatcca ccaggatggc gtgctgaaaa accgcgaaaa ctacgaaatc atgacaccag
     1441 aatctattgg tctgaaccaa atccagctga atctgacctc tcgttcgggg cgtgcggcgg
     1501 tgaaacatcg catggatgag atggggtata aagaaagtga atataattta gacaatttgt
     1561 acgatgcttt cctgaagctg gcggacaaaa aaggtcaggt gtttgattac gatctggagg
     1621 cgctggcctt catcggtaag cagcaagaag agccggagca tttccgtctg gattacttca
     1681 gcgtgcagtc tggctctaac gatatcgcca ccgccgccgt caaactggcc tgtggcgaag
     1741 aagtcaaagc agaagccgcc aacggtaacg gtccggtcga tgccgtctat caggcaatta
     1801 accgcatcac tgactataac gtcgaactgg tgaaatacag cctgaccgcc aaaggtcacg
     1861 gtaaagatgc gctgggtcag gtggatattg tcgccaacta caacggtcgc cgcttccacg
     1921 gcgtcggcct ggccaccgat attgtcgagt cctccgccaa agccatggtg cacgtactga
     1981 acaatatctg gcgtgccgca gaagtcgaaa aagagttgca acgcaaagct caacacaacg
     2041 aaaacaacaa ggaaaccgtg tgatgtcgaa gaattaccat attgccgtat tgccggggga
     2101 cggtattggt ccggaagtga tgacccaggc gctgaaagtg ctgaatgccg tgcgcaaccg
     2161 ctttgcgatg cgcatcacca ccagccatta cgatgtaggc ggcgcagcca ttgataacca
     2221 cgggcaacca ctgccgcctg cgacggttga aggttgtgag caagccgatg ccgtgctgtt
     2281 tggctcggta ggcggcccga agtgggaaca tttaccacca gaccagcaac cagaacgcgg
     2341 cgcgctgctg cctctgcgta agcgcttcaa attattcagc aacctgcgcc cggcaaaact
     2401 gtatcagggg ctggaagcat tctgtccgct gcgtgcagac attgccgcta acggcttcga
     2461 catcctgtgc gtgcgcgaac tgaccggcgg catctatttc ggtcagccaa aaggccgcga
     2521 aggtagcgga caatatgaaa aagcgtttga taccgaggtg tatcaccgtt ttgagatcga
     2581 gcgtattgcc cgcatcgcgt ttgaatctgc tcgtaaacgt cgccacaaag tcacctcaat
     2641 cgacaaagct aacgtgctgc aatcctctat tttatggcgt gaaatcgtca acgagatcgc
     2701 cacggaatac ccggatgtcg aactggcgca tatgtacatc gacaacgcca ccatgcagct
     2761 gattaaagat ccatcacagt ttgacgttct gctgtgctcc aacctgtttg gcgacattct
     2821 gtctgacgag tgcgcaatga tcactggctc gatggggatg ttgccttccg ccagcctgaa
     2881 cgagcaaggt tttggtctgt atgaaccggc aggcggctca gcaccagata tcgcaggcaa
     2941 aaacatcgcc aacccgattg cgcaaattct gtcgctggca ctgctgctgc gctacagcct
     3001 ggatgccgat gatgcggctt gcgccattga acgcgccatt aaccgcgcat tagaagaagg
     3061 cattcgcacc ggggatttag cccgtggcgc tgccgccgtt agtaccgatg aaatgggcga
     3121 tatcattgcc cgctatgtgg cagaaggggt gtaatcatgg ctaaaacgtt atacgaaaaa
     3181 ttattcgacg ctcacgtagt gtacgaagcc gaaaacgaaa ccccgctgtt atatatcgac
     3241 cgccacctgg tgcatgaagt gacctcaccg caggcgttca atggtctgcg cgcccacggt
     3301 cgcccggtac gtcagccggg caaaaccttc gccactatgg atcacaacgt ctctacccag
     3361 acgaaagaca ttaatgcctg tggtgaaatg gcgcgcatcc agatgcagga gctgattaaa
     3421 aactgcaaag aatttggcgt cgaactgtat gacctgaatc acccgtatca ggggatcgtc
     3481 cacgtaatgg ggccggaaca gggcgtcacc ttgccgggga tgaccattgt ctgcggcgac
     3541 tcgcataccg ccacccacgg cgcgtttggc gcactggcct ttggtatcgg cacttccgaa
     3601 gttgaacacg tactggcaac gcaaaccctg aaacagggcc gcgcaaaaac catgaaaatt
     3661 gaagtccagg gcaaagccgc gccgggcatt accgcaaaag atatcgtgct ggcaattatc
     3721 ggtaaaaccg gtagcgcagg cggcaccggg catgtggtgg agttttgcgg cgaagcaatc
     3781 cgtgatttaa gcatggaagg tcgtatgacc ctgtgcaata tggcaatcga aatgggcgca
     3841 aaaaccggtc tggttgcacc ggacgaaacc acctttaact atgtcaaagg ccgtttgcat
     3901 gcgccgaaag gcaaagattt cgacgacgcc gttgcctact ggaaaaccct gcaaaccgac
     3961 gaaggcgcaa ctttcgatac cgttgtcact ctgcaagcag aagaaatttc accgcaggtc
     4021 acctggggca ccaatccagg ccaggtgatt tccgtgaacg acaatattcc cgatccggct
     4081 tcgtttgccg atccggttaa acgcgcgtcg gcagaaaaag cgctggccta tatggggctg
     4141 aaaccgggca ttccgctgac cgaagtggct atcgacaaag tgtttatcgg ttcctgtacc
     4201 aactcgcgca ttgaagattt acgcgcggca gcggagatcg ccaaagggcg aaaagtcgcg
     4261 ccaggcgtgc aggcactggt ggttcccggc tctggcccgg taaaagccca ggcggaagcg
     4321 gaaggtctgg ataaaatctt tattgaagcc ggttttgaat ggcgcttgcc tggctgctca
     4381 atgtgtctgg cgatgaacaa cgaccgtctg aatccgggcg aacgttgtgc atccaccagc
     4441 aaccgtaact ttgaaggccg ccaggggcgc ggcgggcgca cgcatctggt cagcccggca
     4501 atggctgccg ctgctgctgt gaccggacat ttcgccgaca ttcgcaacat taaataagga
     4561 gcacaccatg gcagagaaat ttatcaaaca cacaggcctg gtggttccgc tggatgccgc
     4621 caatgtcgat accgatgcaa tcatcccgaa acagtttttg cagaaagtga cccgtacggg
     4681 ttttggcgcg catctgttta acgactggcg ttttctcgat gaaaaaggcc aacagccaaa
     4741 cccggacttc gtgctgaact tcccgcagta tcagggcgct tccattttgc tggcacgaga
     4801 aaacttcggc tgtggctcct cgcgtgagca cgcgccctgg gcattgaccg actacggctt
     4861 taaagtggtg attgcgccga gttttgctga catcttctac ggcaatagct ttaacaacca
     4921 gctgctgccg gtgaaattaa gcgatgcaga agtggacgaa ctgtttgcgc tggtgaaagc
     4981 caatccgggg atccatttcg acgtggatct ggaagcgcaa gaggtgaaag cgggagagaa
     5041 aacctatcgc tttaccatcg atgccttccg ccgccactgc atgatgaacg gtctggacag
     5101 tattgggctt accttgcagc acgacgacgc cattgccgct tatgaagcaa aacaacctgc
     5161 gtttatgaat taatcccctt gcccggtcaa atgaccgggc tttccgctat cgtccacgtc
     5221 atcaaacgtc tttaaccttt gcggttaaaa ataatgcgac aacagaaata accgcaatta
     5281 cccagtagac
//
LOCUS       DADMKF010000212         5281 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.216, whole genome shotgun sequence.
ACCESSION   DADMKF010000212 DADMKF010000000
VERSION     DADMKF010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5281
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.216"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(<1..471)
                     /locus_tag="JNC24_003796"
     CDS             complement(<1..471)
                     /locus_tag="JNC24_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012562879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAY9160440.1"
                     /translation="MKHIQIRNSDMAWHIAANIQFPPNFDESKQYPAIISVHPFGSCK
                     EQTSGNIYGKALAEKGYLVLAYDASFQGESGGEPRWIEDPTQRVEDISRVIDYAVTLP
                     YVNAERIGVLGVCGGGGYAINAALTEKRIKAVVSITGVNIGRLFREGFSNYDPIG"
     gene            complement(471..1391)
                     /locus_tag="JNC24_003797"
     CDS             complement(471..1391)
                     /locus_tag="JNC24_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAY9160441.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYQAIVVSHPGG
                     GVKEQTAGTYAKKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDISAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPYVEAGSNARTSDISSGEYAVMPLAPMKESDAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITFDAYHMAEVYLTQPMQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKAYVAEAISVLAPFFEETL"
     gene            1529..2428
                     /locus_tag="JNC24_003798"
     CDS             1529..2428
                     /locus_tag="JNC24_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY9160442.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMAQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPMLHDIDSALASLSELQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAGVRLGGEMDKDMIAIRI
                     GPDIPMAIVGSPDYFSRRSVPTSVSQLIDHQAINLYLPTSGTANRWRLIRGGREVRVR
                     MEGQLLLNTIDLIIDAAIDGHGLAYLPYDQVERAIKEKKLIRVLDKFTPDLPGYHLYY
                     PHRRHAGSAFSLFIDRLKYKGAV"
     gene            2765..4378
                     /locus_tag="JNC24_003799"
     CDS             2765..4378
                     /locus_tag="JNC24_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY9160443.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKDRV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFTPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSTHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(4429..>5281)
                     /gene="ynaI"
                     /locus_tag="JNC24_003800"
     CDS             complement(4429..>5281)
                     /gene="ynaI"
                     /locus_tag="JNC24_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HAY9160444.1"
                     /translation="INYVIENYKLKFITPDVIDFICTSLIAVILTIKLFLLINQFEKQ
                     QIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAGKD
                     ILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPNSL
                     FSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLVYF
                     NQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMDNI
                     TPPEQGR"
BASE COUNT         1429 a         1203 c         1226 g         1423 t
ORIGIN      
        1 gccaatcgga tcgtaattgc tgaaaccttc ccgaaaaaga cgcccgatat taacacccgt
       61 aatactcact actgctttaa ttcgtttttc tgtcagcgcg gcattaatgg catagccacc
      121 acccccacac actcccagca cgccaatacg ttcagcatta acatatggca gagttactgc
      181 ataatcgata acacggctaa tatcctctac gcgctgtgtc ggatcttcta tccagcgagg
      241 ctccccacca gattctccct ggaaactggc gtcataagcg agaacgagat agcctttttc
      301 agccaatgcc ttgccataga tattgccaga cgtctgctcc ttacaactgc caaaaggatg
      361 gacgctgata atcgccggat actgttttga ctcatcaaaa tttggtggaa actgaatatt
      421 ggcagcaata tgccaggcca tatcactatt gcggatttga atatgtttca ttacagagtt
      481 tcctcaaaga acggagctaa tactgaaatt gcttcagcca cataggcttt gccgtcataa
      541 agatccatat ggtttgcgcc ttcaacaatg tgatagcgtt tatcttggct tgatgctcga
      601 tcgtacaggt cgtcactcat ccatttgctc cctgcctggc tgcccgccac aatctgcatc
      661 ggctgagtca ggtacacttc cgccatatgg taagcatcaa aggtaataat ctggttaagg
      721 ctgcgcaaag tagcgtaacc cggtgctgtt ggatactgcg cgcgaggggt gtggtaatac
      781 tcccaggcct gacgcagttc ttcgttcggc gcatcggact ccttcattgg tgccagtggc
      841 ataacggcat attctccgct gctaatatcg ctggttcggg cgtttgaacc cgcttcaacg
      901 tatggcaggg catcaataga tttcacattg ttttcccaac cattacggaa catcgaacca
      961 atattgaccg cactaacggt accgatggcc ttgatgcggc gatcctgaat tgcagcattg
     1021 gctgtatatc ctgcaccggc acaaattccc atcgcaccaa ttcgggtatt gtcgacataa
     1081 gaaagcgttg tcaggtaatc aatcacggca ctgatgtctt cagtacgaat gtatgggttt
     1141 tctaactgac gcggctcgcc gccactttca ccttgataag atgcgtcata agcaatagtg
     1201 acaaatccct tttccgccag ttttttggca taggttccgg ccgtttgttc tttaacgccc
     1261 ccacctgggt gagataccac aattgcctga tactgacggg tttcatcaaa ttttggaggg
     1321 aaatagatca ctgcagacaa agagatggtt ggattattgc tgttagtgaa gctgacttta
     1381 ttattcatca ttccgttcct catggagttg tcggttcgtt ttaacggttg gtgatatcac
     1441 tatagatatt gatcattaag ttgattagac ccaaaattat gattagacct atcatttaaa
     1501 tgattaatag attgcatgga gatactgaat gaagcgtgaa gaaatcgctg atctgatggc
     1561 gtttgtcgtc gttgcagagg agcgtagctt cactcgtgca gcagcccgcc tgagcatggc
     1621 gcagtcagct ttaagccaga tagtgcgtcg tatagaagaa cgattgggat tgcggcttct
     1681 gacgcgaacc acgcgcagcg ttgttccaac tgaagcgggc gagcatcttt tgtctgttct
     1741 tggccctatg ttgcatgaca tagattcagc cctggcatcc ctgagcgagc tgcagaaccg
     1801 cccatccggg acaatacgta ttactactgt agaacatgca gccaaaacga tattgttacc
     1861 agcaatgcgc acattcctga aatcgcatcc tgaaattgat attcagctca ccattgatta
     1921 tggtttgacc gatgtcgttt ctgaacgttt tgatgcaggc gtccgtctgg gtggggagat
     1981 ggataaagat atgatcgcca ttcgaatcgg gccagatata ccaatggcta ttgttggctc
     2041 accggattat ttttctcgcc gaagtgttcc aacgtcagtg tcacaattaa tagatcatca
     2101 ggcaattaat ttgtatcttc ccacatcggg tacagcaaat cgctggagat taatacgcgg
     2161 tggacgtgaa gttcgtgttc gcatggaagg tcagctttta ctgaatacga tagacctgat
     2221 cattgatgct gcaattgatg ggcatggatt ggcgtatcta ccttatgatc aggttgagcg
     2281 ggctattaaa gaaaaaaaac tgatacgtgt tttggataaa ttcacaccag atttacccgg
     2341 ttatcacctg tactatccac accgtcgaca tgctggctcg gcattctcat tatttataga
     2401 taggctgaag tataaaggtg ctgtttagca ctacttgctg atacattaat ttaatttttc
     2461 tcttaacgta ttctcagttc ctttcaacgt tttggttatt ttttattctt cgtacaatgg
     2521 cgacagatgc tgattatgat accgaaaact ggtttgaacg tgcgaagcct gaacgtagtg
     2581 ttcggtgttc tatgtgcttt accgcatttt gtcgtctatt atttacacta aatctgattt
     2641 aatatattga tacataaaac attttatgct ccctgtgtca cttttttgat ggaagttgtt
     2701 tgcatttctt taaggcgaaa caaataatta cgcatcaatt ttaatgtcgg ttagagggaa
     2761 acttatgaag cactctgttt cagtcacgtg ttgtgcgctg ttggtcagca gcatttctct
     2821 ttcgtatgct gcagaagttc cgagcggcac agtactggca gagaagcagg agctggtgcg
     2881 ccacattaaa gatgagcctg cgtcgctgga tcccgctaaa gccgtgggcc tgccagagat
     2941 tcaggtcatt cgcgatctgt ttgaaggact ggtgaatcag aacgagaaag gggagattgt
     3001 ccccggcgtt gcgactcagt ggaaaagtaa tgacaaccgt atctggactt ttaccctgcg
     3061 cgataacgca aaatgggcgg atggcacacc ggtaacggcg caagattttg tctacagctg
     3121 gcagcgtctg gtggacccga aaacgctatc gccatttgca tggtttgccg cgctggcggg
     3181 aatcaacaac gcacaggcga ttattgatgg taaagctacg ccagaccagc ttggcgtcac
     3241 cgcagttgat gcccatactt tgaaaattca gcttgataaa ccgttgccgt ggtttgtgaa
     3301 tttaaccgct aactttgcct tcttcccggt gcaaaaagcc aacgtagaaa gcggtaaaga
     3361 gtggacgaaa cccggaaatc tgatcggcaa tggcgcttat gttcttaaag atcgcgtagt
     3421 caatgaaaaa ctggtcgtgg taccgaatac ccattattgg gataacgcca aaacggtact
     3481 gcaaaaagtg accttcctgc caattaatca ggaatccgca gccactaagc gttacctcgc
     3541 gggggatatt gatatcaccg aatccttccc gaaaaatatg tatcagaagc tgttgaagga
     3601 tattccgggg caggtttata cgccgccgca gctcgggacc tattattatg cgtttaacac
     3661 gcaaaaaggg ccgacggcag atcagcgcgt tcgtctggca ttaagtatga cgatagatcg
     3721 ccgcctgatg accgaaaaag tattagggac gggcgaaaag ccagcgtggc attttacacc
     3781 agatgttacc gccggattta cgccggaacc ttcgccgttt gaacaaatga gtcaggaaga
     3841 actgaatgcg caggcaaaaa ctttgttgag cgcagctggt tatggtccgc aaaaaccgct
     3901 gaagctgacg cttttgtata acacttcaga aaaccatcaa aaaattgcga ttgctgtagc
     3961 atcgatgtgg aaaaagaacc ttggcgtaga tgttaaattg caaaatcagg aatggaaaac
     4021 ctatatcgat agccgtaaca ccggcaattt tgatgttatc cgcgcctcat gggtggggga
     4081 ttataatgaa ccctccactt tcctgacatt attaacgtca acgcattcag gaaatatttc
     4141 acgctttaac aatccggcat acgacaaagt tctggcccag gcatcgacgg aaaataccgt
     4201 taaagcgcgt aatgccgatt acaacgcggc agaaaaaatc ctcatggagc aagcaccgat
     4261 tgcaccaatt tatcaatata ccaatggacg attaatcaag ccgtggctga aaggttatcc
     4321 cattaataac ccggaagatg tggcgtacag ccggactatg tatattgtga agcattgatg
     4381 tgatgggaac tggcgttacc cttgtggata acgccagtga tgtctgattt agcgcccctg
     4441 ttccggcggt gtaatgttat ccatatacag cgtctggctg gggaaggcaa agtccgcgcc
     4501 gtgtgactgc acaatatcga taatcttcaa ataaacgtct tgctgtgcag caagccattc
     4561 agcccatacc gtggttttgg taaagcaata aaccataata ttcaatgacg agtcggcaaa
     4621 ctggttgaaa taaaccagta aggtttgtcg ctggtcgatg gccgggtgat ttttcagcat
     4681 ctcacgtacg gcttcgacaa taacgcccac ttttgccgca tcctcataac gtaaaccaat
     4741 ggtcgtggta atgcggcggt tggtcattcg tcctgggttt tctacgctga tcgacgaaaa
     4801 cagcgagttc ggtacgtaca atggacgatt atcaaaggtc gtaattttgg taattcgcca
     4861 gccaatttcc gctactgtac cttcgatatt tctgtccggt gaacggatcc agtcgccaat
     4921 actgaaagga cggtcgaaat agagcatgat cccggaaaag aaattactca gaatatcttt
     4981 accggccata ccgacagcca gaccaccaat accaccaaag gtcagcaagc cagaaaggct
     5041 catgccgaaa tgttcgccat aaagcagaac aagcaccaca ataatggtga ttttgataat
     5101 gcgcgacata atccgcgcac tggtgatatc gcgacctttt ttaatctgct gtttttcaaa
     5161 ctgattaatc agcaaaaata gcttaatcgt cagaataacc gcaatcaggg acgtacagat
     5221 aaaatcgata acgtctgggg tgataaattt gagtttatag ttttctataa cataattaat
     5281 a
//
LOCUS       DADMKF010000213         5244 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.217, whole genome shotgun sequence.
ACCESSION   DADMKF010000213 DADMKF010000000
VERSION     DADMKF010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5244
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.217"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(<1..1008)
                     /locus_tag="JNC24_003801"
     CDS             complement(<1..1008)
                     /locus_tag="JNC24_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014334055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAY9160445.1"
                     /translation="MRLTAVYSCVRVLAESVGMLPCSLYKISGTLKTRAVDERLHKLV
                     SAKPNGYMTPQEFWELVIVCLCLRGNFYAYKVKALGEVVELLPIDPGCVEPKLNSQWQ
                     PVYQVTFPDGSVDVLTQNEIWHVRTLTLDGLVGLNPIAYAREAISLAAATEEHGARLF
                     SNGAVTSGVLRTEQKLTPDAYERLKKDFEECHTGLGNAHRPMILEMGLDWKSMALNAE
                     DSQFLETRKFQLEEICRLFRVPLHMVQNTDRATFNNIEELGLGFINYSLVPYLTRIEQ
                     RINTGLVRKSKQGVYYAKFNAGALLRGDMKSRFEAYATGINWGIYSSNDCRDLEDMNP
                     RP"
     gene            complement(1133..1315)
                     /locus_tag="JNC24_003802"
     CDS             complement(1133..1315)
                     /locus_tag="JNC24_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9160446.1"
                     /translation="MIMLILAPLVGVLGALLLAYGAWLIYPPAGFVVAGALCLFWSWL
                     VARYLDRTRQSVGGGK"
     gene            complement(1327..3060)
                     /locus_tag="JNC24_003803"
     CDS             complement(1327..3060)
                     /locus_tag="JNC24_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAY9160447.1"
                     /translation="MSRKSYPNVNAANQYARDVVRGKIVACQFVIQACQRHLDDLMAE
                     KSKSFRYRFDKDLAERAAKFIQLLPHTKGEWAFKRMPITLEPWQLFVICCAFGWVNKG
                     TRLRRFREVYTEIPRKNGKSAISAGVALYCFACDNEFGAEVYSGATTEKQAWEVFRPA
                     RLMCKRTPMLTEAFGIEVNASNMNRPEDGARFEPLIGNPGDGSSPHCAVVDEYHEHAT
                     DALYTTMLTGMGARRQPLMWAITTAGYNIEGPCYDKRREVIEMLNGSVPNDELFGIIY
                     TVDEGDDWTGPQVLEKANPNIGVSVYREFLLSQQQRAKNNARLANVFKTKHLNIWVSA
                     RSAYFNLVSWQSCEDKSLTLEQFEGQPCILAFDLARKLDMNSMARLYTREIDGKTHYY
                     SVAPRFWVPYDTVYSVEKNEDRRTAERFQKWVEMGVLTVTDGAEVDYRYILEEAKAAN
                     KISPVSESPIDPFGATGLSHDLADEDLNPVTIVQNFTNMSDPMKELEAAIESGRFHHD
                     GNPIMTWCIGNVVGKNMPGNDDVVKPVKEQAENKIDGAVALIMAVGRAMLYEKEDTLS
                     DHIESYGIRSL"
     gene            complement(3057..3551)
                     /locus_tag="JNC24_003804"
     CDS             complement(3057..3551)
                     /locus_tag="JNC24_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003832359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HAY9160448.1"
                     /translation="MAGTAGRSGRRPKPTARKALAGNPGKRALNKDEPVFTPIKGVEP
                     PEWFAEEDLPLATIMWQLTTKELCGQGLLCVTDLAVLERWCVAYEFWRRAVKNIAIQG
                     NTITGAMGGRVKNPELTAKKEQESEMSSTGAMLGLDPSSRQRLIGLAGQKKATNPFLK
                     IIES"
     gene            complement(3673..4023)
                     /locus_tag="JNC24_003805"
     CDS             complement(3673..4023)
                     /locus_tag="JNC24_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAY9160449.1"
                     /translation="MPSLIPRACRKRGCAGTTTDSSGYCDKHRGEGWVQHQRGLSRHQ
                     RGYGSKWTVIRARILKRDKGLCQLCLRVGVVSEAKTVDHIIPKAHGGTDADSNLQSLC
                     WPCHKAKTARERIR"
     gene            complement(4066..4530)
                     /locus_tag="JNC24_003806"
     CDS             complement(4066..4530)
                     /locus_tag="JNC24_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HAY9160450.1"
                     /translation="MSRVTAIISALVICIIVCLSWAVNHYRDNAITYKEQRDKNAREL
                     KLANATITDMQQRQRDVNALDAKYTKELADAKAENETLRADVAAGRRRLHIKAVCQSV
                     REATTASGVDNATSPRLADTAERDYFTLRERLMTMQMQLEGAQEYIRTQCIK"
     gene            complement(4527..5024)
                     /locus_tag="JNC24_003807"
     CDS             complement(4527..5024)
                     /locus_tag="JNC24_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HAY9160451.1"
                     /translation="MSPSLRKAVAAAIGGGAVAIASVLITGPGGNDGLEGVSYIPYKD
                     IIGVWTVCHGHTGKDIMLGKTYTEAECKALLNKDLATVARQINPYIKVDIPETTRGAL
                     YSFVYNVGAGNFRTSTLLRKINQGDIKGACDQLRRWTYAGGKQWKGLMTRREIEREVC
                     LWGQQ"
     gene            complement(5024..5239)
                     /locus_tag="JNC24_003808"
     CDS             complement(5024..5239)
                     /locus_tag="JNC24_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAY9160452.1"
                     /translation="MYQMEKISTGIAYGTSAGSAGYWFLQWLDQVSPSQWAAIGVLGS
                     LLLGLLTYLTNLYFKIREDKRKAARGE"
BASE COUNT         1148 a         1534 c         1299 g         1263 t
ORIGIN      
        1 gggacgcgga ttcatatctt ccaggtcgcg gcagtcattg gaagagtaaa ttccccagtt
       61 gatcccggtg gcgtaggctt caaaacggga cttcatatcc ccgcgcagta acgccccggc
      121 gttaaatttg gcgtaataaa cgccctgctt actttttcgt accagtccgg tgttgatccg
      181 ctgttcgatg cgggtcagat acggcaccag tgaatagttg ataaatccca gccccagctc
      241 ttcgatattg ttgaaggtgg cgcgatcggt gttctgcacc atgtgcaacg gcacccggaa
      301 cagacgacag atttcttcca gctgaaactt gcgggtttcc aggaactgac tgtcctcggc
      361 gttcagcgcc atcgacttcc agtccagccc catttccaga atcatcgggc ggtgagcatt
      421 gccaagcccc gtgtgacact cctcaaaatc tttcttcaga cgctcgtaag catccggcgt
      481 gagcttttgt tccgtacgca acacaccgga tgtcaccgca ccattactaa acaacctggc
      541 accgtgctcc tcggttgccg ctgccagtga aatggcctca cgcgcatacg caatgggatt
      601 cagcccgaca agtccatcca gcgtcagggt gcgcacatgc cagatttcat tctgggtcaa
      661 cacatccacg gaaccatccg gaaacgtcac ctgataaacc ggctgccact ggctgttcag
      721 cttcggttcc acacagcccg gatctatcgg aagaagctcc accacttctc ccagtgcctt
      781 taccttgtag gcgtaaaaat tcccccgtag acacaggcag acaataacca gctcccagaa
      841 ttcctgcggt gtcatgtagc cattaggttt tgccgaaacc aacttatgca gtcgttcatc
      901 caccgcccgt gttttcaggg taccgctgat tttgtagaga ctgcagggca gcataccaac
      961 agactctgcc agcaccctga cgcaagaata gaccgccgtc aggcgcatgg cccgctggct
     1021 actgatccgc tttccggtat aggtgtcgta tgacagcccg atagcatcag cagttctgct
     1081 ggcgtggtca ccggcacatc gctttttcgt tgaaataatc ccgaaaagaa cactatttac
     1141 ctccgccgac agactgccgt gtacggtcga gatatcgcgc caccagccac gaccagaaca
     1201 ggcacagcgc cccggcaaca acaaaacccg ccgggggata aatcagccag gcaccatacg
     1261 ccagcaaaag cgcacccagc acgcccacca gaggcgcgag aatcagcatg atcataatta
     1321 cctcagttaa agcgagcgga tcccgtagga ctcaatgtgg tcagacagcg tgtcttcttt
     1381 ctcgtacagc atggctctgc caaccgccat aatcagcgca actgcaccat cgattttgtt
     1441 ttccgcctgc tctttgacgg gtttcaccac atcatcgtta cccggcatgt ttttgccgac
     1501 cacgttgccg atacaccagg tcatgatggg attgccgtca tgatgaaaac gtcccgattc
     1561 aatcgctgct tccagctctt tcatcggatc ggacatattg gtgaagttct ggacgatagt
     1621 gacgggattc aggtcttcat cagcaaggtc atgtgacaac ccggtcgccc cgaaggggtc
     1681 gatgggtgac tcactgaccg ggctgatttt gttcgccgct ttggcctcct cgaggatgta
     1741 gcgataatcc acctccgcac catcggtaac ggtcagaacg cccatttcca cccatttctg
     1801 aaagcgttcg gctgtccgtc gatcttcatt tttctcgacg ctgtacaccg tgtcatacgg
     1861 tacccagaaa cgcggggcca cactgtagta atgcgtttta ccgtcaatct cgcgggtata
     1921 aagtcgcgcc atgctgttca tatccagctt acgcgccagg tcaaaggcca gaatgcacgg
     1981 ctgcccctcg aactgctcaa gggtcagtga tttatcctcg cagctctgcc agctcaccaa
     2041 gttgaaatac gccgaacgcg ccgacaccca gatattgagg tgttttgttt taaagacgtt
     2101 tgccagacgg gcgttatttt tcgcacgctg ctgctgactt aacaaaaatt cgcgataaac
     2161 cgacacgcca atatttggat tggctttttc cagcacctgc gggccggtcc agtcgtcacc
     2221 ttcatcaacg gtatagatga tcccgaacag ttcatcgtta ggcaccgagc cgttgagcat
     2281 ctcgatgact tcccgccgct tgtcgtagca cggcccctca atgttgtacc cggcggtggt
     2341 gatggcccac atcagtggct gacgtcgcgc ccccatcccg gtaagcattg tggtataaag
     2401 cgcatcggtg gcatgctcgt gatattcatc aaccacggca cagtggggtg atgaaccatc
     2461 acctgggttg ccgatcagcg gttcaaaccg cgcgccatcc tccggacggt tcatgtttga
     2521 ggcgttaacc tcaatcccga acgcttccgt cagcatgggt gtgcgtttac acatcagtcg
     2581 cgccgggcga aagacttccc acgcctgttt ctctgtcgtg gcaccggaat acacttccgc
     2641 gccaaactcg ttatcacagg caaaacaata cagggcaaca ccggcagaga ttgctgattt
     2701 gccgttctta cgggggattt cggtgtacac ctcccggaag cggcgcaacc gggtgccttt
     2761 attgacccag ccaaacgcac agcagatcac aaatagctgc cacggctcca gcgtgatggg
     2821 catccgtttg aatgcccact cccccttggt gtgcggcaac agctgaataa atttcgcggc
     2881 ccgttcagcc aggtccttgt cgaagcggta acgaaacgac ttactttttt ccgccatcag
     2941 gtcatcaaga tggcgctggc aggcctgaat cacaaactgg caggcaacaa tctttccgcg
     3001 cacgacatcc cgggcatact gatttgcagc atttacgttg gggtaagatt tccggctcat
     3061 gattcgataa ttttcagaaa cgggttagtg gctttcttct gccccgccag gccaatcaga
     3121 cgctggcggc tgctggggtc gagtccgagc attgcccccg tgctgctcat ctcggactcc
     3181 tgttcttttt tggcggtcag ctccggattt ttgaccctgc cgcccattgc accggtgatg
     3241 gtgttgccct gtatggcaat atttttcacg gcacgtcgcc agaactcata ggccacgcac
     3301 caccgctcaa gtaccgccag gtcagtcacg cacagcaggc cctgaccgca gagttctttg
     3361 gttgtcagtt gccacatgat cgtggcgaga gggagatctt cttcagcgaa ccactctggt
     3421 ggctcaacac ctttgatggg cgtaaaaaca ggttcatctt tgttcagggc tcgcttgccg
     3481 gggtttccgg ccagcgcctt gcgcgccgtt ggcttggggc gacgcccgga acgccccgcc
     3541 gttccagcca tatgcggcac tcctggttaa atttcatttt tcgcgggtat aaaaaaacga
     3601 tggggcgggc agtccggaag acgtcaggtc acagggattt gccccgcccc tccctacaag
     3661 tgagaataat tatcaccgga ttcgttcgcg cgctgttttc gctttatggc agggccagca
     3721 cagactctgc aggttgctgt ctgcgtctgt tccgccatgc gctttcggga tgatgtggtc
     3781 gacggttttc gcctcgctca ccacaccgac acgcagacac aactgacaca gacctttatc
     3841 gcgcttcaga atacgggcac gaatcaccgt ccattttgag ccatagccac gctggtggcg
     3901 gctcagtccg cgctggtgct gcacccagcc ttcaccgcga tgtttatcgc agtaacccga
     3961 actgtctgtg gttgtacctg cacatccacg cttacggcag gcacgtggga ttagtgatgg
     4021 cataaatacc tcataccctg cgaaatgttt accacgataa aaaggctact taatgcactg
     4081 agtgcggata tactcctgtg ccccttccag ttgcatctgc atcgtcatca gccgctctct
     4141 gagggtgaaa taatcccgtt cagcggtgtc tgccagtcgg gggctggttg cattatccac
     4201 gccggaggcg gtggtggctt cacgcactga ctgacagact gctttgatgt gcaaccgacg
     4261 acgaccagcg gcaacatcag cgcgcagagt ttcattttca gctttcgcat cagctaactc
     4321 cttcgtgtat ttagcatcga gtgcattaac atcacgctgt ctctgctgca tgtcagtaat
     4381 ggttgcgttc gccagcttca gttctcttgc gtttttgtcg cgctgctctt tgtaggtaat
     4441 ggcgttatca cggtaatgat taacagccca tgacaggcag acgatgatgc agataaccag
     4501 agcggagata atcgcggtga ctctgctcat tgctgccccc acaaacagac ttcacgctca
     4561 atctcacgac gggtcatcag ccctttccat tgcttaccgc cagcatatgt ccagcgacgt
     4621 agctggtcac atgcgccttt gatatcgccc tggtttattt tgcgaagaag cgtcgatgtt
     4681 ctgaaattgc cagcgcccac gttgtaaacg aacgagtaaa gagcgccgcg cgttgtttcc
     4741 ggtatatcga ctttgatgta cgggttaatt tgtctggcga cagtggcaag gtctttattc
     4801 aggagggctt tgcattctgc ttcggtatac gttttaccga gcatgatgtc ttttccggtg
     4861 tgtccgtgac atacagtcca tacaccaatg atatctttgt atggtatgta gctgacacct
     4921 tccagaccat cgttaccacc tgggccagtg attaacacag atgctatagc aacagcccca
     4981 ccaccaatag cagctgcaac agccttgcgt aatgatggcg acattattca cctctcgcag
     5041 ccttacgctt gtcttctctt attttgaaat acagatttgt cagataagtc agaagcccca
     5101 gaagcagact tcccagcaca ccaatcgcag cccactgtga tggactgacc tgatccaacc
     5161 actgcaaaaa ccagtagccg gcactgccgg cggaggtgcc gtaggcaatg cctgttgata
     5221 ttttttccat ctgatacata tccc
//
LOCUS       DADMKF010000214         5184 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.218, whole genome shotgun sequence.
ACCESSION   DADMKF010000214 DADMKF010000000
VERSION     DADMKF010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5184
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.218"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(<1..580)
                     /locus_tag="JNC24_003809"
     CDS             complement(<1..580)
                     /locus_tag="JNC24_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V subunit UmuC"
                     /protein_id="HAY9160453.1"
                     /translation="MFALADINSFYASCEKVFRPDLRNEPVIVLSNNDGCVIARSPEA
                     KALGIRMGQPWFQVRQMRLEKKIHVFSSNYALYHSMSQRVMAVLESLSPAVEPYSIDE
                     MFIDLRGINHCISPEIFGHQLREQVKSWTGLTMGVGIAPTKTLAKSAQWATKQWPQFS
                     GVVALTAENRNRILKLLGLQPVGEVWGVGRRLT"
     gene            complement(580..1017)
                     /gene="umuD"
                     /locus_tag="JNC24_003810"
     CDS             complement(580..1017)
                     /gene="umuD"
                     /locus_tag="JNC24_003810"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HAY9160454.1"
                     /translation="MSTVYHRPAAPSGDDSYVRPLFADRCQAGFPSPATDYAEQELDL
                     NSYCISRPAATFFLRASGESMNQAGVQNGDLLVVDRAEKPQHGDIVIAEIDGEFTVKR
                     LLLRPRPALEPVSDSPEFRTLYPENICIFGVVTHVIHRTRELR"
     gene            complement(1014..1262)
                     /locus_tag="JNC24_003811"
     CDS             complement(1014..1262)
                     /locus_tag="JNC24_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ImpC"
                     /protein_id="HAY9160455.1"
                     /translation="MIRIEILFDRQSTKNLKSGTLQALQNEIEQRLKPHYPEIWLRID
                     QGSAPSVSVTGARNDKDKERILSLLEEIWQDDSWLPAA"
     gene            1612..2583
                     /locus_tag="JNC24_003812"
     CDS             1612..2583
                     /locus_tag="JNC24_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HAY9160456.1"
                     /translation="MRHGLMEAACERRIPMPNWCSNRMYFSGEPAQIAEIKRLASGAV
                     TPLYRRATNEGIQLFLAGSAGLLQIAENIRSEQCPGVTAAGRGAVSPENIAFTRWLTH
                     LQNGVLLDEQNCLMLHELWLQSGTGQRRWEGLPDDVRDTITALFTAKRGDWCGFWSNE
                     DVSVWWNRLCDNVLPEKTMPFDLLTVLPTRLDIEVNGFNGGVLNGVPSAYHWYTERYG
                     VKWPCGYDLNISSQGENFIQVDFDTPWCQPESDVVAELSRLFGCTLEHWYAEQGCDFC
                     GWQLYERGELVDVLWGELEWSSPTDDDELPEVTGPAWIVDNVAHYGG"
     gene            2587..2891
                     /locus_tag="JNC24_003813"
                     /pseudo
     CDS             2587..2891
                     /locus_tag="JNC24_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052644.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2967..3650
                     /locus_tag="JNC24_003814"
     CDS             2967..3650
                     /locus_tag="JNC24_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HAY9160457.1"
                     /translation="MSRFVLGNCIDVMARIPDNAIDFILTDPPYLVGFRDRQGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFMSAWKNAGFSVVGHLVFTKNYT
                     SKAAYVGYRHECAYILAKGRPRLPQNPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLTAVQRAMQQGAAN
                     DNWFEPEAA"
     gene            3651..3872
                     /locus_tag="JNC24_003815"
     CDS             3651..3872
                     /locus_tag="JNC24_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9160458.1"
                     /translation="MNYAGHEKLRADVAEVANTMCDLRARLNDMEHRCRFDSDVLVER
                     LTRQTLYRANRLFMEAYTEILELDSCFKD"
     gene            3886..4320
                     /locus_tag="JNC24_003816"
     CDS             3886..4320
                     /locus_tag="JNC24_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 domain-containing protein"
                     /protein_id="HAY9160459.1"
                     /translation="MYGTCETLCRALAAKYPGDTPLMLVIWSPEEIQALADGMDISLS
                     DHEIRTVLARLEDIPEDQRTESGISSGAAMEIINNVRENRQVTVPAELLASLIQTAEQ
                     ALWKREWAARDNGLAVPECVTRRQAVVNQARALLKNNTREND"
     gene            4365..5051
                     /locus_tag="JNC24_003817"
     CDS             4365..5051
                     /locus_tag="JNC24_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9160460.1"
                     /translation="MNETLNALICRHARNLLDAPRLATLLINRHGGVLPPLLASAIQK
                     LTGTGAELVLSGSQWQSLPVLPADGTQVSFPYAGEWLTEDEIRAVLDAVHDAVRSVSC
                     RVAEDARRIRAALTTTGQTLLTRQTRRFRLVVKESDHPCWLDEDDENLPVVLDAILNR
                     GARFSSVEMYLVSECVEHILSSGLACDVLRIPDEPSRRWFDRDILREVVLEARTEIRS
                     MADALAKIRK"
BASE COUNT         1093 a         1417 c         1501 g         1173 t
ORIGIN      
        1 ccgtcagtct gcgtcctact ccccagacct caccaactgg ctgcaacccc agtagcttca
       61 agatccgatt acggttttct gccgtcagcg cgaccacacc ggaaaactgt ggccattgct
      121 ttgttgccca ctgggcactt ttagccagcg tttttgtagg cgcaatgccc acccccatgg
      181 tgagtcctgt ccagctcttt acctgttccc tgagctgatg accaaaaatc tccggagaga
      241 tgcaatgatt tatcccccgc aaatcaatga acatttcatc aattgagtag ggctcaactg
      301 cgggagaaag cgactccaga acagccataa cccgttggct catgctgtgg tacagcgcat
      361 aattgctgga aaatacatgt attttcttct ccaggcgcat ttgtctcacc tgaaaccagg
      421 gctgccccat tctgatgcca agggcttttg cctccgggct gcgcgcgatc acacagccat
      481 cgttattgct gagtacgatg accggttcgt tgcgaaggtc cgggcggaaa actttttcac
      541 atgaggcgta gaaactgttg atatcagcca gtgcaaacat cagcgtaact cccgcgtcct
      601 gtgtatcacg tgagtgacaa caccaaaaat acagatgttt tccggataca gtgtgcggaa
      661 ctccgggctg tctgaaaccg gctccagtgc cgggcgtggg cgcaacagca gtcgtttgac
      721 ggtgaactca ccgtcgatct cagcgataac gatgtccccg tgttgtggtt tttcggccct
      781 gtccactacc agcagatcac cattctgcac gccagcctgg ttcatcgatt caccgctggc
      841 gcgcagaaag aaggtggctg caggtctgct gatgcaatag ctgttcagat ccagttcctg
      901 ctcagcataa tcagtggcag gcgaaggaaa accggcctgg caacgatcgg caaacaacgg
      961 tcgcacgtaa ctgtcatcgc ctgaaggggc agccggacgg tgataaacgg tactcatgct
     1021 gcaggcagcc agctgtcgtc ctgccagatt tcttccagca gagacaggat acgttcttta
     1081 tccttgtcgt tgcgggctcc tgtgacagat acagacgggg cgctgccctg gtctatacgc
     1141 agccagattt ccgggtagtg tggtttcaga cgttgttcga tttcattctg tagcgcctga
     1201 agtgtgcctg atttgaggtt ttttgtgctc tggcggtcga aaagaatttc aatgcggatc
     1261 atgttctcat ctcccttttc tttatgctgt atgtttatac agtattctaa taactgaatc
     1321 cgcacacagt caaatgtcat tgttgtgact aaaaaacgct caattctctg agtgtgtggc
     1381 tgtatgcctg ctgtgggctg ctgtgtccag cttatccaca gcattttgtg cacggttctg
     1441 tggacaaaat acctggttac ccaggccgtg ccggcacgtt aaccgggctg catccgatgc
     1501 aagtgtgtcg ctgtcgccgg cctcctcacc cggtcacgtt tcgtcgttcc tcctccacgc
     1561 gctgcggctt cggggccgca cctgcattcg tatgcggtcg cccggtaaca ggtgcggcac
     1621 ggcctgatgg aggccgcatg tgagaggaga attcccatgc caaactggtg ctcgaatcgt
     1681 atgtattttt ctggtgaacc ggcacagatc gctgagatta aacgactggc cagcggtgca
     1741 gttacaccgc tttatcgccg cgccacaaat gaaggtattc agctgtttct ggccggaagt
     1801 gccggacttc tgcagatcgc tgaaaatatc cgctctgagc agtgccccgg agtgacggct
     1861 gccggacgtg gtgctgtatc accggaaaat atcgcgttca cccgctggct gacacacctg
     1921 cagaacggtg tactgctgga tgagcaaaac tgcctgatgc tgcatgaact ctggctgcag
     1981 agcggtactg gacagcgtcg ctgggaagga ttaccggacg atgtcaggga taccatcacc
     2041 gcacttttca ccgcaaaaag aggtgactgg tgtggcttct ggagtaacga ggatgtatcg
     2101 gtgtggtgga accgtctgtg tgacaacgta ctgccggaaa aaaccatgcc gtttgacctg
     2161 ctgacggttt tgccgacccg cctggatatt gaagtgaatg gctttaacgg cggtgttctg
     2221 aacggtgttc cttctgcata tcactggtat acagaacggt atggcgtgaa gtggccttgt
     2281 gggtatgacc tgaatatcag cagtcagggg gaaaacttca ttcaggtaga tttcgacacg
     2341 ccgtggtgtc agccggaaag cgacgttgtt gcagaattaa gccgcctttt cggctgcacg
     2401 ctggagcact ggtacgccga acagggctgt gatttctgtg gctggcagtt gtatgagcgc
     2461 ggagagcttg ttgatgtgct gtggggggaa ctggaatggt cttccccgac agatgacgat
     2521 gagctgccgg aagtcaccgg acctgcgtgg atagtcgaca atgtggcgca ttatggcgga
     2581 tgaagtatga cggagacggg cgggcaaccg cccgtttctt ttccggtaaa ggatgtcgcc
     2641 gtgctcctct ttttactgcg ccggctgacg cggcgcggca taaacgctgc ctgtggtcag
     2701 tgtcccgcgt ccggtgggca cgggacgggc gcttttaccg ctcccggctg gcaccggtga
     2761 cagtgtacgc cagcctgtcg cccttttctg atgaacgccc ttcaagccgc tttcgcggca
     2821 tagccttgcc gtcaaaaaga cgacggctgc ggtattccac ggtcggcctg acccgttacc
     2881 ggacgcggtg aacagcccac aggcagcggg gaacgggcac cacaggggtg ccctcccggt
     2941 gccctctgta agagaaggag tttggtatgt cccgttttgt cctcggtaac tgcatcgatg
     3001 ttatggcccg tatccctgat aacgccattg atttcatcct caccgacccg ccgtacctcg
     3061 tcggttttcg tgaccgtcag gggcgtacca ttgccggcga taaaaccgat gaatggctgc
     3121 aaccggcctg taatgagatg taccgcgtac tcaaaaaaga cgcgttaatg gtgagcttct
     3181 atggctggaa tcgcgtcgat cgttttatgt ctgcctggaa aaatgcggga ttcagcgttg
     3241 ttggtcacct ggtcttcacc aaaaactaca catcgaaggc cgcatatgtg ggctatcgcc
     3301 acgaatgtgc ctacatcctg gcaaaaggcc gtccacgtct gccacaaaac ccgctgccgg
     3361 acgtgctggg ctggaaatat tcgggcaatc gccatcaccc gacggaaaag cccgttacca
     3421 gcctgcaacc actgattgag agcttcacac atccgaacgc gattgtgctg gacccgtttg
     3481 caggcagcgg atcaacctgc gtggccgctc ttcagtccgg acgccggtat atcggtatcg
     3541 agctgcttga acagtatcac cgtgccggac agcaacgact gaccgccgtg caacgggcca
     3601 tgcagcaggg ggccgcgaat gataactggt ttgaaccgga ggctgcgtaa atgaactatg
     3661 ccggacacga aaaacttcgc gccgacgtgg cggaggtggc caacaccatg tgtgaccttc
     3721 gggccaggct gaacgatatg gagcatcgct gtcgctttga ttccgatgtg cttgttgaac
     3781 gcctgacacg ccagacgctg tatcgcgcca atcgcctgtt catggaagcg tataccgaaa
     3841 ttctggaact ggattcctgc ttcaaagatt gagaaggaga cgagaatgta cggaacatgc
     3901 gaaacgctct gccgggcgct ggcagcaaag tatccgggag atacgccgct gatgctggtt
     3961 atctggtccc cggaagaaat tcaggccctc gctgacggaa tggatatttc cctgtccgat
     4021 catgaaatca ggactgtcct ggcgcgcctg gaggacatcc cggaagacca gcggactgaa
     4081 tccggtattt cttccggtgc ggcgatggag atcatcaata acgtgagaga aaaccgccag
     4141 gtgaccgtcc ctgctgaact gctggcgtcc ctgattcaga ccgccgaaca ggcactgtgg
     4201 aaacgtgagt gggccgcccg tgataacggc ctcgccgtcc cggaatgcgt cacccgccga
     4261 caggcggtgg ttaatcaggc ccgcgccctg ctgaaaaaca acacacgcga aaacgactga
     4321 tgttatcgcc gcctgcgggc ggcgattcag ggagaccatg aatcatgaac gaaacgctga
     4381 atgcactgat ttgtcgccac gcccgtaacc tgctggatgc gccccggctg gcgacgttac
     4441 ttatcaatcg tcacggcggc gtactgccgc cgctcctggc ctctgccatt cagaaactga
     4501 ccggaaccgg ggcggaactt gttctgtccg gcagtcagtg gcagtcgctg ccggtacttc
     4561 cggcagacgg aacgcaggtg tctttcccgt atgccggaga atggctgacc gaagacgaaa
     4621 tcagggcagt tcttgatgcg gtgcacgatg cggtacgcag tgtcagttgc cgggtggcag
     4681 aagatgcgcg gcgtatcagg gcggcgctga ccacaaccgg tcagacgttg ctgacccgcc
     4741 agacgcgccg ctttcgcctg gttgtgaagg aaagcgatca cccctgctgg ctcgatgaag
     4801 acgacgaaaa cctgcccgtg gtgctcgatg ccatcctgaa ccggggcgct cgtttttcgt
     4861 cggtggaaat gtatctggtc agtgagtgtg tggagcatat tctgtccagt gggctggcct
     4921 gcgatgtgct gcgtatacct gatgaaccgt cccgtcgctg gtttgaccgg gatattctgc
     4981 gggaggtggt cctggaagcc cggactgaaa tccgcagcat ggcggatgca ctggcgaaaa
     5041 tccggaaatg atgacgctgg cggggaaaaa tctccgcctt ttccagccgg tccccggaaa
     5101 gagcaaaagg atgtcgccgc aggctccgct ttacccggcc atgcggggca tggccttgtg
     5161 ggttttcagt tatgtggcct cagc
//
LOCUS       DADMKF010000215         5044 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.219, whole genome shotgun sequence.
ACCESSION   DADMKF010000215 DADMKF010000000
VERSION     DADMKF010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5044
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.219"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            165..1601
                     /gene="lsrA"
                     /locus_tag="JNC24_003818"
                     /pseudo
     CDS             165..1601
                     /gene="lsrA"
                     /locus_tag="JNC24_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553891.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
     gene            1595..1903
                     /locus_tag="JNC24_003819"
     CDS             1595..1903
                     /locus_tag="JNC24_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000911157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY9160461.1"
                     /translation="MLKFIQNNREITALLAVVLLFVLPGFLDRQYLSVQTLTMVYSSA
                     QILILLAMGATLVMLMRKVVSAMISWIILSRARQKYGAASVVVDEQSWLSSGVKKSPY
                     "
     gene            complement(2075..3523)
                     /locus_tag="JNC24_003820"
     CDS             complement(2075..3523)
                     /locus_tag="JNC24_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HAY9160462.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAISYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFAL
                     AALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDHVIGTQELVAIVLAEKDHWEQ
                     DLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            complement(3750..4700)
                     /locus_tag="JNC24_003821"
     CDS             complement(3750..4700)
                     /locus_tag="JNC24_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HAY9160463.1"
                     /translation="MHVHPISTFRLFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGGFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLVINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPLTVMLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGL
                     VARMGGEEFAVAVPSVNPVDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRAS
                     YRTLTDDFNKLMVEADTCLYRSKKDGRNRTSTMRYGEEVV"
     gene            complement(4839..>5044)
                     /locus_tag="JNC24_003822"
     CDS             complement(4839..>5044)
                     /locus_tag="JNC24_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4186 family protein"
                     /protein_id="HAY9160464.1"
                     /translation="ALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSL
                     SEEQQRYIVAVIYHWLVVQMNQP"
BASE COUNT         1316 a         1288 c         1229 g         1211 t
ORIGIN      
        1 taaggttatg aacaaattaa aagcagaaat acatttgttc aaaactcacc tgcaaaactg
       61 aacgggggaa atatgcaaac gagtgatacc cgcgcgttac cgctactttg cgcccgctcg
      121 gtttataaac agtattcagg ggtcaatgtc ctgtcgattt tacgttgcat caaggggagg
      181 tccacgccct gctcggcggc aatggtgccg gtaaatcgac gttaatgaag attattgccg
      241 gtattacccc tgctgatagc ggtacgctgg agattggggg caacaactac gtcagattaa
      301 cgccagttca tgctcatcag ctgggtattt atctcgttcc ccaggaaccg ctgcttttcc
      361 caagcctgtc gataaaagaa aacatcctgt ttgggctggc aaaaaaacag ctctccatgc
      421 agaaaatgaa gaacttgctg gcggcgctgg gctgctagtt tgatctgcat agtctggcag
      481 gatcgctgga tgtcgccgat cgccaaatgg tggaaatcct ccgcggcctg atgcgcgact
      541 cgcggattct gatcctcgat gaacctaccg cctcgcttac ccctgcggaa accgaacgct
      601 tgtttcgtcg cttgcaagag ctgcttgcta ctggcgtggg tattgttttt atctcgcata
      661 agctgccgga aattcgccag attgccgatc gaattagcgt gatgcgcgac ggaaccatcg
      721 ccttaagcgg caaaaccagc gaactgtcta ccgatgacat tattcaggcc atcacgccag
      781 tggtacggga aaaatcgctc tctgccagcc aaaaattatg gctggagtta cctggcaacc
      841 gcccacaaca tgccgccgga acgtcggtgc tgacactaga aaatctgacc ggcgaaggtt
      901 tcaggaatgt cagcctgacg ctcaatgccg gagaaattct gggtctggcc gggctggtgg
      961 gggccggacg cacagaactg gccgagacgc tctatggtct gcgtactttg cgtggcggac
     1021 gcattatgct gaatggtaaa gagatcaata aattatccac tggagaacgt ttactgcgcg
     1081 gtctggttta tctgccggaa gatcgccagt catccggact gaatctcgat gcttcgctgg
     1141 cctggaacgt ctgcgccctt actcataacc ttcgtggatt ctgggcgaaa accgcgaaag
     1201 ataatgccac cctggaacgt tatcgtcggg cgctgaatat taaatttaac caaccggaac
     1261 aagctgcacg gacattatcc ggtggcaacc agcaaaaaat cctgattgcc aaatgcctgg
     1321 aagcttcgcc gcaagcattg attgtcgatg agccgacgcg cggcgtggat gtctcggccc
     1381 gtaatgatat ctaccagctg ttgcgcagca tcgccgcaca gaatgtggca gtgctgctta
     1441 tctcctctga tctggaagag atcgaactga tggcagatcg tgtgtatgtg atgcatcagg
     1501 gcgaaattac ccactctgca ctgaccgggc gcgatattaa tgtcgagact attatgcgcg
     1561 ttgccttcgg cgatagtcag cgtcaggagg cgtcatgctg aagtttattc agaacaaccg
     1621 tgaaatcacg gcactgctgg cggtggtgct gctgtttgta ttacccggtt ttctcgaccg
     1681 ccagtattta agtgtgcaaa cgctgaccat ggtttatagc agcgcgcaaa tcctgatcct
     1741 gctggcaatg ggcgcgacgc tggtaatgct tatgcgcaaa gtagtaagcg cgatgatatc
     1801 ttggataata ctgagccgtg ccagacaaaa atacggcgca gccagtgttg ttgtggacga
     1861 acaatcatgg ttatcttcag gcgtgaaaaa gtcgccctat taaacgtgat tacgatcaca
     1921 ttctcaagac gctacttaca aattacctac aagcttacaa cattaacagg cgctgcatgt
     1981 ggcatctgtt aatgataaag gcacatagga tgttgtaact aatatagtga aataagaaac
     2041 ccggtcaaaa ccgggttcag aagtaacggt gttattagca caacggacgt acagcttcgc
     2101 gcatcccttt ttcaagaatc gcatccaggt cattagcaac ctgctcgacc agaccaggca
     2161 cttgcgtcag gtcctgctcc cagtggtctt tttctgccaa cacaatcgct accagttcct
     2221 gggtaccgat aacatggtca cgatgctggc tccagagttg ctgataacgt tccagccagt
     2281 gtgcatcgtc ctgtaccgga tatgtttctc cgttacgctc accacgatag aacgcaatca
     2341 atgccgctaa tgcgaaagta aggcgcgccg gaagtgtgcc gtttgccttc tgccctgcca
     2401 gcagctgcgg caggatgcgg gtgcggaact tggtcatacc gttgagcgcg atagacagca
     2461 gctgatgctt atacgggtta cggaaacgcc cggtgactgc actggcgaaa gattccagtt
     2521 catcacgagg caaatccagt accggaataa tttcttcgta gatggctttt tcaacgaatg
     2581 cgcaaatttc agcatcgttc atcgcttcac ctacggtatc cagccctgcc tggaacgcca
     2641 ccggcaccag cgcggtgtgc gcaccgttga ggattgccac tttgcgttct ttatacggtt
     2701 taatatcgtc aacaatcagc acgttgagcg gatatttgtc caggcgcagc tcagtcgcta
     2761 aggatttcgg cccctgaatc acaaacaggt aaaagtgttc agcggtgtcg aggaaaccat
     2821 cgtgataacc cagttcttct tccagtttag ccacttcatc gcgcggataa ccggtaacga
     2881 tacggtccac cagcgtagaa cagaagctgt tagcctgatc cagccattga ataaatgctt
     2941 ctggcagtgc ccactcttgc gcatagcgca gcaccagttc acgcaacgcg tcgccattgt
     3001 agtcgatcaa ctcacacggg atgatgatcc aacctttatc cagcgcaccg ttgaaatggc
     3061 taaagcgttc gaacagcaga cgggtcagtt ttgccggata gcttatcgct ggcgcgtcat
     3121 cgaatttatc gcccgcatgg tagctgatcc ccgcttcggt ggtgttagag aaaacaaagc
     3181 gcatttccgg gttgtgcgcc agtttcagga attcatcgta ttcactgtag acgctgattt
     3241 cacgattaac cgagcgaatc agacgcgcgt cgctgaccgc ttcccccttc tcattcaggc
     3301 cacggataat ggtggtatac agaccatcct gcgtgctcag tgacggcggg aatgaagttt
     3361 caatcggacg aacaacgacc acgccagaat tcagatcggt gtgctcattc aggagatcga
     3421 tttgccagtc aacaaaggcg cgcaggaagt taccttcacc aaactgaatg atacgttctg
     3481 gatactgtgc accgggaaaa tcgcgacgat ttagtgtttt cacaatgggt tcccttctga
     3541 ttagtcatac aacctgtttt aattggtacg acaggttagc aaactttaat acgccgaacc
     3601 cctgttttga tcaactcctg atgattaatg agcagtttta tgagaaaagt gtggcgcgga
     3661 tcatggttta atcgaggaaa tacgcctttt cctggatcat aaagtggtag aacacattgc
     3721 attcaaatcg cgcggaatga ataaagatgt cagacaactt cctcaccgta acgcatagtg
     3781 ctggtacggt tgcgcccatc tttcttcgag cgatacagac acgtatcggc ttcgaccatc
     3841 aatttattaa aatcatcggt cagcgttcgg tacgatgcgc gaccactacc gacgccaata
     3901 cttaccgtga gataaagcgt tttttgttgc caggtgaatg gttgcagttc aacgccttta
     3961 cggatttttt ccgccattag cagaccatcg acaggattca ccgacggcac tgcaacagca
     4021 aattcttcgc cgcccatgcg cgccaccagc cccttatcac cgacaatctt ctgaatatgc
     4081 cgggcaaaca cgcttaacac tttatcgcca cattcatgtc catagttatc gttaatgctt
     4141 ttgaaatagt cgatatcaag cagcatgacc gtcagaggca cgatcaacac gcccgttgag
     4201 aattgctcac tgaaccagtc cgcaagcaaa ggccagaaat ccagactatc aatactgacc
     4261 gaaccaatcg ccccgacaat agcgcataat aatgcggcaa tcagacagta attaaatagc
     4321 cgtaaggcac ttactggctc atacttattt ttccccagac gcttatcccg cacgaccagt
     4381 aaggcgacgg taacaataaa catcatattg gagaaattga taaccagtga aaccagtccc
     4441 cattcggtgg ttatggcatc ataaaccagc atcgccacat aactgatcgc atagtaatgc
     4501 agtcgattaa gccagacata gcgggcaaac ccccccgcca ttacgccatt cagcggccag
     4561 aaaagagaaa gttcgtgaac cagccgtaac tctgcaccaa taaaatagaa cagcgtggtt
     4621 agcgcaaaaa tagcgatgct attacgtagc agatgacctt cctggaacaa acggaaagtt
     4681 gaaatggggt gtacatgcat taattatctg tccatacaat tgcgggtggc aattatctca
     4741 acgtaataag taaattctca taaaggatag catgttcgct aacagcggcg gcaaataaag
     4801 gtaccctggg aatgctattt tatcgttttc cgcaattctc atggctgatt catctgtaca
     4861 accagccagt gatagattac cgccacgata taacgttgtt gttcttcgct taacgaaaca
     4921 ccctgcggaa tgttatgcca cttagccaga caaccgcgac agcaggtcgc ggtggcgtgc
     4981 tgagcgataa acaccggatg cccgcgcatg ggggtttgct taccgtcatt agccggtaat
     5041 gccg
//
LOCUS       DADMKF010000216         5039 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.220, whole genome shotgun sequence.
ACCESSION   DADMKF010000216 DADMKF010000000
VERSION     DADMKF010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5039
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.220"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            82..197
                     /gene="rrf"
                     /locus_tag="JNC24_003823"
     rRNA            82..197
                     /gene="rrf"
                     /locus_tag="JNC24_003823"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(315..758)
                     /locus_tag="JNC24_003824"
     CDS             complement(315..758)
                     /locus_tag="JNC24_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAY9160465.1"
                     /translation="MVISIRRSRHEEGEELVAIWCRSVDATHDFLSAEYRAELEVLVR
                     SFLPEAPLWVAVNERDQPVGFMLLSGQHMDALFIDPDVCGCGVGRMLVEHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVEE"
     gene            915..1844
                     /gene="metA"
                     /locus_tag="JNC24_003825"
     CDS             915..1844
                     /gene="metA"
                     /locus_tag="JNC24_003825"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HAY9160466.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYFRDVEAGLDPDVPYNYFPHNDPQNKPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            2113..3714
                     /gene="aceB"
                     /locus_tag="JNC24_003826"
                     /pseudo
     CDS             2113..3714
                     /gene="aceB"
                     /locus_tag="JNC24_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418438.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
     gene            3744..4964
                     /gene="aceA"
                     /locus_tag="JNC24_003827"
                     /pseudo
     CDS             3744..4964
                     /gene="aceA"
                     /locus_tag="JNC24_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005137734.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
     gene            4937..5035
                     /locus_tag="JNC24_003828"
     CDS             4937..5035
                     /locus_tag="JNC24_003828"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HAY9160467.1"
                     /translation="MGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
BASE COUNT         1260 a         1318 c         1287 g         1174 t
ORIGIN      
        1 tgttttggcg gatgagagaa gattttcagc ctgatacaga ttaaatcaga acgcagaagc
       61 ggtctgataa aacagaattt gcctggcggc cgtagcgcgg tggtcccacc tgaccccatg
      121 ccgaactcag aagtgaaacg ccgtagcgcc gatggtagtg tggggtctcc ccatgcgaga
      181 gtagggaact gccaggcatc aaattaagca gaaggccatc ctgacggatg gcctttttgc
      241 attggcgcag aaaaaaatgc cgggtgcgac gctggcgcgt cttctccagc ctgggcaggc
      301 acggcagggg cagcttattc ctccacatac gccagattca gcaacggata cggtttcccc
      361 aaatcgtcca cctcagagcg tcccgtaacc ttaaaaccca ccttcttata gaacccaacc
      421 gcctgctcat tttgctcatt aacgttggtt gtcagttccg gtgccatcga gagcgcatgc
      481 tccaccagca tccgacccac gccgcagccg cacacatcag gatcgataaa cagcgcatcc
      541 atatgctgcc cacttaacaa cataaaccca accggctgat cccgttcatt aaccgcgacc
      601 cacaacggcg cttccggcag gaaggaacga actagcacct ccagctcggc ccgatactct
      661 gctgataaaa aatcgtgtgt ggcatcgaca gaacgacacc aaatcgcaac gagttcctcc
      721 ccttcctcat gccgtgagcg gcgaatacta ataaccattt tctctccttt tcgtcattct
      781 tatattctaa cgtagccttt tccttgaaac tttctcacct tcaacatgca ggctcgacat
      841 tggcaaattt tctggttatc ttcagctatc tggatgtcta aacgtataag cgtatgtagt
      901 gaggtaatca ggttatgccg attcgtgtgc cggacgagct acccgccgtc aatttcttgc
      961 gtgaagaaaa cgtctttgtg atgacaactt ctcgtgcgtc tggtcaggaa attcgtccac
     1021 ttaaggttct gatccttaac ctgatgccga agaagattga aactgaaaat cagtttctgc
     1081 gcctgctttc aaactcacct ttgcaggtcg atattcagct gttgcgcatc gattcccgtg
     1141 aatcgcgcaa cacgcccgca gagcatctga acaacttcta ctgtaacttt gaagatattc
     1201 aggaacaaaa ctttgacggt ctgatagtta ccggcgcgcc gctgggcctg gtggagttta
     1261 atgatgtcgc ttactggccg cagatcaaac aggtgctgga gtggtcgaaa gatcacgtca
     1321 cctcgacgct gtttgtctgc tgggcggtac aggccgcgct caatatcctc tacggcattc
     1381 ctaagcaaac tcgcaccgac aaactctctg gcgtttacga gcatcatatt ctccatcctc
     1441 atgcgcttct gacgcgtggc tttgatgatt cattcctggc accgcattcg cgctatgctg
     1501 actttccggc agcgttgatt cgtgattaca ccgatctgga aattctggca gagacggaag
     1561 aaggggatgc atatctgttt gccagtaaag ataagcgcat tgcctttgtg acgggccatc
     1621 ccgaatatga tgcgcaaacg ctggcgcagg aatatttccg cgatgtggaa gccggactag
     1681 acccggatgt accgtataac tatttcccgc acaatgatcc gcaaaacaaa ccgcgcgcga
     1741 gctggcgtag tcacggtaat ttgctgttta ccaactggct caactattac gtctaccaga
     1801 tcacgccata cgatctacgg cacatgaatc caacgctgga ttaatcttct gtgatagtcg
     1861 atcgttaagc gattcagcac cttacctcag gcaccttcgg gtgccttttt tatttccgaa
     1921 acgcacctca gcaagtgaat aaattttatt catattgtta tcaataagtt atcaagtatt
     1981 tttaattaaa atggaaattg tttttgattt tgcattttaa atgagtagtc ttagttgtgc
     2041 tgaacgaaaa gcgcacaacg atccttcgtt cacagtgggg aagttttcgg atccatgacg
     2101 aggagctgca cgatgactga acaggcaaca acaaccgatg aactggcttt cacaaggccg
     2161 tatggcgagc aggagaagca aattcttact gccgaagcgg tagaatttct gactgagctg
     2221 gtgacgcatt ttacgccaca acgcaataaa cttctggcag cgcgcattca gcagcagcaa
     2281 gatattgata acggaacgtt gcctgatttt atttcggaaa cagcttccat tcgcgatgct
     2341 gattggaaaa ttcgcgggat tcctgcggac ttagaagacc gccgcgtaga gataactggc
     2401 ccggtagagc gcaagatggt gatcaacgcg ctcaacgcca atgtgaaagt ctttatggcc
     2461 gatttcgaag attcactggc cccggactgg aacaaagtga tcgacgggca aattaacctg
     2521 cgtgatgcag ttaacggcac catcagctat accaatgaag caggcaaaat ttatcagctc
     2581 aagcccaatc cagcggtttt gatttgtcgg gtacgcggtc tacacttgcc ggaaaaacat
     2641 gtcacctggc gtggtgaggc aatccccggt agcctgtttg attttgcgct ctatttcttc
     2701 cacaactatc aggcactgtt ggcaaagggc agcggtccct atttctatct gccgaaaacc
     2761 cagtcctggc aggaagcggc ctggtggagc gaagtcttca gctatgcaga agatcgcttt
     2821 aatctgccgc gcggcgccat caaggcgacg ttgctgattg aaacgctgcc cgccgtgttc
     2881 cagatggacg aaatccttca cgcgctgcgc gaccatattg ttggtctgaa ctgcggtcgc
     2941 tgggattaca tcttcagcta tatcaaaacg ttgaaaaact atcccgatcg tgtcctgcca
     3001 gacagacagg ccgtgacgat ggataaacca ttcctgaatg cttactcacg cctgctgatt
     3061 aaaacctgcc ataaacgcgg tgcttttgcg atgggcggca tggcagcgtt tattccgagc
     3121 aaagatgaag agcgtaataa ccaggtgctc aacaaagtaa aagcggataa agcgctggaa
     3181 gtcaataacg gtcacgatgg cacatggatc gctcacccag gccttgcgga cacggcaatg
     3241 gcggtattca acgacattct cggctcccgt aaaaatcagc ttgaagtatt gcgcgaacaa
     3301 gacgcgccga ttactgccga tcagctgctg gcaccttgtg acggtgaacg caccgaagaa
     3361 ggtatgcgcg ccaacattcg cgtggctgtg cagtacatcg aagcgtggat ctccggcaac
     3421 ggctgcgtgc cgatttatgg cctgatggaa gatgcggcga cggctgaaat ttcccgtacc
     3481 tcgatctggc agtggatcca tcatcaaaaa acgttgagca atggcaaacc ggtgaccaaa
     3541 gccttgttcc gctagatgct gggcgaagag atgaaagtca ttgccagcga actgggcgaa
     3601 gaacgtttct cccagggccg ttttgacgat gccgcacgct tgatggaaca gatcaccact
     3661 tccgatgagt taattgattt cctgaccctg ccaggctacc gcctgttagc gtaaaccacc
     3721 acataactat ggagcatctg cacatgaaaa cccgtacaca acaaattgaa gaattacaga
     3781 aagagtggac tcaaccgcgt tgggaaggca ttactcgccc atacagtgcg gaagatgtgg
     3841 tgaaattacg cggttcagtc aatcctgaat gcacgctggc gcaactgggc gcagcgaaaa
     3901 tgtggcgtct gctgcacggt gagtcgaaaa aaggctacat caacagcctc ggtgcactga
     3961 ctggcggtca ggcgctgcaa caggcgaaag cgggtattga agcagtctat ctgtcgggat
     4021 ggcaggtagc ggcggacgct aacctggcgg ccagcatgta tccggatcag tcgctctatc
     4081 cggcaaactc ggtgccagct gtggtggagc ggatcaacaa caccttccgt cgtgccgatc
     4141 agatccaatg gtccgcgggc attgagccgg gcgatccgcg ctatgtcgat tacttcctgc
     4201 cgatcgttgc cgatgcggaa gccggttttg gcggtgtcct gaatgccttt gaactgatga
     4261 aagcgatgat tgaagccggt gcagcggcag ttcacttcga agatcagctg gcgtcagtga
     4321 agaaatgcgg tcacatgggc ggcaaagttt tggtgccaac tcaagaagct attcagaaac
     4381 tggtcgcggc gcgtctggca gctgacgtga cgggcgttcc aaccctactg gttgctcgta
     4441 ccgatgccga tgcagcggat ctgatcacct ccgattgcga cccgtatgac agcgaattta
     4501 ttaccggcga gcgtaccagt gaaggcttct tccgtactca tgcgggcatt gagcaagcga
     4561 tcagccgtgg cctggcgtat gcgccatatg ctgacctggt ctggtgtgaa acctccacgc
     4621 cggatctgga actggcgcgt cgctttgcac aagctatcca cgcgaaatat ccgggcaaac
     4681 tgctggctta taactgctcg ccgtcgttca actggcagaa aaacctcgac gacaaaacta
     4741 ttgccagctt ccagcagcag ctgtcggata tgggctacaa gttccagttc atcaccctgg
     4801 caggtatcca cagcatgtgg ttcaacatgt ttgacctggc aaacgcctat gcctagggcg
     4861 agggtatgaa gcactacgtt gagaaagtgc agcagccgga atttgccgct ataccttcgt
     4921 atctcaccag caggaagtgg gtacaggtta cttcgataaa gtgacgacta ttattcaggg
     4981 cggcacgtct tcagtcaccg cgctgaccgg ctccactgaa gaatcgcagt tctaagcaa
//
LOCUS       DADMKF010000217         5026 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.221, whole genome shotgun sequence.
ACCESSION   DADMKF010000217 DADMKF010000000
VERSION     DADMKF010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5026
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.221"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            12..754
                     /locus_tag="JNC24_003829"
                     /pseudo
     CDS             12..754
                     /locus_tag="JNC24_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308314.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
     gene            complement(796..1248)
                     /locus_tag="JNC24_003830"
                     /pseudo
     CDS             complement(796..1248)
                     /locus_tag="JNC24_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            1312..1458
                     /locus_tag="JNC24_003831"
                     /pseudo
     CDS             1312..1458
                     /locus_tag="JNC24_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612015.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1458..2615)
                     /gene="intS"
                     /locus_tag="JNC24_003832"
     CDS             complement(1458..2615)
                     /gene="intS"
                     /locus_tag="JNC24_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage integrase IntS"
                     /protein_id="HAY9160468.1"
                     /translation="MLTVKQIEAAKPKEKPYRLLDGNGLYLYVPVSGKKVWQLRYKID
                     GKEKILTVGKYPLMTLQEARDKAWTARKDISVGIVPVKAKKASSNNNSFSAIYKEWYE
                     HKKQVWSVGYATELAKMFDDDILPIIGGLEIQDIEPMQLLEVIRRFEDRGAMERANKA
                     RRRCGEVFRYAIVTGRAKYNPAPDLADAMKGYRKKNFPFLPADQMPAFNKALATFSGS
                     IVSLIATKVLRYTALRTKELRSMLWKNVDFENRIITIDASVMKGRKIHVVPMSDQVVE
                     LLTTLSSITKPVSEFVFAGRNDKKKPICENAVLLVIKQIGYEGLESGHGFRHEFSTIM
                     NEHEWPADAIEVQLAHANGGSVRGIYNHAQYLDKRREMMQWWADWLDEKVE"
     gene            complement(2777..2851)
                     /locus_tag="JNC24_003833"
     tRNA            complement(2777..2851)
                     /locus_tag="JNC24_003833"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2816..2818),aa:Arg,seq:cct)
     gene            complement(2927..3859)
                     /locus_tag="JNC24_003834"
     CDS             complement(2927..3859)
                     /locus_tag="JNC24_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HAY9160469.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHVELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            4153..4908
                     /gene="mlaA"
                     /locus_tag="JNC24_003835"
     CDS             4153..4908
                     /gene="mlaA"
                     /locus_tag="JNC24_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HAY9160470.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADGLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
BASE COUNT         1226 a         1166 c         1174 g         1460 t
ORIGIN      
        1 gggtggatga tatggcaact acatgttcag ttatattgat tttggagtcc tttgatgttt
       61 atttcggaaa agagagtgtg tttctggaga gaggttcatc tgtacttgtc gactctagct
      121 ctagagattt tttcctgaca tatcctgaaa gagtgatagt ggcggatttt ggcgctgagt
      181 ttattagtcg ctatttgaaa gctaataact taagggatat ttctgattgt agggaatatc
      241 tatcttattt aaaaataaac tttgctgact tcagtttaat taaaggatta attagttggg
      301 ctaatcactg tgctgaatac atagaaattt ttgatgagtc tattgctttt acatgtctct
      361 ctgcattttc ttctgaaaaa caatttggag tatttctgtt tggatgtttg aaaagcacag
      421 ggctaaagtt aaaacgatta ttcatacgga tttatctgca ccatggcgtc ttaaggatat
      481 atcatcaaga ttatatctca gcgaaagttt actaaagatg aaattgaaag aagagggggt
      541 atcattcagt aagatcatac ttgatgagag gatgcaaatg gctgaatatt tactcagcac
      601 tcgttgttat cctattagta aagtagctaa ggtctgtggt tatgccagtg tctcatactt
      661 tacttatgta tttagacgtt attttggtgt ttctccaagt caatactctc agaggagttc
      721 agaaagtaaa attcttactc accagggaat ctgatcattg ttcttgcccc cttatttcca
      781 gacagggggt gtatcttaag ttaacgttac ccgctgacgt cgatattctc gcggagagcg
      841 ataacccagc gcactgtgcg gatggttttc attgtaatgt tcgatcgcca ctgcaagatt
      901 atgcaatgcc gttcttacat tcggtttcgg catgaacgcg atgtagtctt ccttcatcgt
      961 tttcacgaac ctttctgcca ttccattact ctgaggactg ctgattgccg ttgtgcaggg
     1021 ctccagattc aactctctgg cgaactgccg tgtttcatgc gctctatacg ctgaaccgtt
     1081 atctgtcagc cactggatgg actgttccgg tactttatcg ccaaagcgtt tttctatcgc
     1141 tcctaacatg acatcctgca ctgtcgcttt atcgtaacct cccgtgcttg cggcccagtc
     1201 tatggcttca cgatcgcaac agtccagggc gaacgtaaca cgcagttttg aatcgccacg
     1261 ggtttaacag acacctcaga gtcatttaag atggcttaaa gagaggtgcc catgagcggt
     1321 aagcgttatc ccgaagagtt taaaactgaa gcagtcaaac aggttgttga tcgcggttat
     1381 tctgttgcca gcgttgcaac acgtctcgat atcactaccc acagccttta tgcctggata
     1441 aagaagtacg gtccggatta ctccactttt tcatcaagcc aatccgccca ccactgcatc
     1501 atttctctgc gcttatcgag atactgagca tggttgtaaa taccgcgcac agatccgccg
     1561 ttggcatgtg ccagctgcac ttcaatagcg tcagcaggcc attcgtgctc gttcataatc
     1621 gtgctgaatt catgcctgaa tccgtgaccg ctttccagac cttcatagcc gatttgtttg
     1681 atcacaagca ataccgcgtt ctcgcagatt ggcttcttct tatcgttgcg cccggcaaaa
     1741 acaaactctg agactggttt ggtgattgag cttagcgtag tgagaagttc aaccacctgg
     1801 tctgacatcg ggaccacatg aattttgcgt cccttcatca cactggcgtc gatggtgata
     1861 atcctgtttt caaaatcgac gttcttccat agcatggaac gaagctcttt tgttcttagg
     1921 gctgtgtagc gtaaaacttt ggtcgcaatg agcgatacga tacttcctga aaatgttgcc
     1981 agtgctttgt tgaatgcagg catctggtct gctggaagga acgggaagtt cttcttgcgg
     2041 tatcctttca tggcgtctgc aaggtcaggt gccgggttat atttagccct tccggtgaca
     2101 atagcgtaac gaaaaacctc gccgcatctt ctgcgggctt tgttggctcg ctccattgca
     2161 ccgcgatctt caaatctgcg gattacttcc agcagttgca tcggctcaat atcctgaatc
     2221 tcaagaccgc cgatgatggg taaaatgtcg tcatcaaaca ttttggcaag ttcagttgca
     2281 tagcctactg accagacttg cttcttgtgc tcgtaccatt ccttgtaaat cgcactaaat
     2341 gaattgttgt tagacgaagc ctttttcgcc tttaccggaa cgatgccaac cgagatgtct
     2401 ttcctcgcag tccatgcctt atcccttgct tcctgcaaag tcataagcgg atattttccg
     2461 acggtcagga ttttctcctt accgtcaatc ttgtagcgaa gctgccatac ctttttcccg
     2521 gatacaggaa cataaaggta caggccatta ccatcgagaa ggcggtatgg tttttctttc
     2581 ggctttgctg cttcaatctg cttaacggtg agcatgggta aaaatccggt gggtaaaatt
     2641 attttatcca ctttttaccc gtcatggagt gcggctgtca acgatctgac gcgaaccatg
     2701 acgaaccgtg aatatacgga aggcttgata ttcaggggat tttgcggact ggtacggatg
     2761 ggagcgaact gataaatggt gtcccctgca ggaatcgaac ctgcaattag cccttaggag
     2821 gggctcgtta tatccattta actaagagga caatgcggca tgagtatacc cgctaatgga
     2881 gtgcggggta agtacgctgc cgctcgattg cttaaaccct cgccatttat gccgggtttt
     2941 tatcattttt cttaatgttt tccgcacgtt ctgctttttg gcgtgcttct gctttacgct
     3001 tgttgctcat gtcgttacga atctgtgcat gactcattaa cgcgaagata aaggtgccgc
     3061 cgcagatgtt ccccgctaaa gtaggtagtg cgaagggcca gatgaaatcg ctccagtgca
     3121 gcgtaccgtt aaacaccaga tagaggattt caacagaacc gaccacgata tgggtggtgt
     3181 cacctagggc aataagccag gtcatcaata taatcaccac aatctttgcc gcacctgctg
     3241 caggaaacat ccataccata gtggcgatca gccagccgga aatgatcgcg ttggcaaaca
     3301 tctcgctggg ggtgttcttc atcacatcca tgccgatttt gacaaatgca tcgcgagttt
     3361 cttcattgaa gataggcata tattcaaatg cccacgcagc aataccagtc ccgagaatat
     3421 tacccagcag cacgactccc cataaccgca taagtaagcc aacgttgctc attgtcggtt
     3481 tttgcatgac gggtagtacc gcagtcacgg tgttttcggt aaataattgc tggcgggcca
     3541 ttatgacgat aataaaacca aaggtataac cgagattctc cagtaagaag ctgcccggca
     3601 caccttccag ttcgacatga aatatccctt ttgccagtaa cgaagcgccc atcgacagac
     3661 ccgccgcaat ggctgaccac agtagcgcca ttgcgtcgcg ttccagctct ttttcaccat
     3721 cctggcggat atgctcatga attgccatcg cccgggaggg gagtcggtct tcatctattt
     3781 ctattttttt gccgcgctct ttttcttcgc tctcaacttc aatttcgtcg ctgtgttgat
     3841 caattttgtc gttgtccatg gtctcttctc ttgaattagc acgtatagct aaagcgtagc
     3901 ggcttttttg ctcgcaactg gcgggagtta ctctgaaaat gtagaaaagg ctgcgtttgc
     3961 ctttttctgt ttctatagaa tcaagtagcc tacagggcgg cgattaccag gctatgatca
     4021 aatcagcaaa tcagggcgtc tggacatcag ttgacgtgct gttacaatcg cccacaccta
     4081 aacaggcgga tacggtatcg ttccgtcatg gatggcaaac tgcataagcc ataaaaaaac
     4141 agggagacat ttatgaagct tcgcctgtcg gcgcttgctc tgggaactac gcttctggtg
     4201 gggtgtgcga gttccggtac agatcagcaa gggcgttctg acccgttaga agggttcaac
     4261 cgcaccatgt acaacttcaa cttcaacgta ttagacccgt atattgttcg accggtcgct
     4321 gtcgcctggc gtgattatgt tccgcaaccg gcgcgtaatg gtttgagcaa ctttactggc
     4381 aaccttgaag aacctgcggt gatggttaac tacttcttgc agggcgaccc ttatcagggg
     4441 atggtccact ttacccgctt tttcctgaac accattttgg ggatgggcgg ctttattgat
     4501 gttgcaggga tggcgaaccc gaaactgcaa cggactgaac ctcaccgctt cggtagtacg
     4561 cttggtcatt atggcgtggg ttatgggcct tacgttcagt taccgttcta cggtagcttc
     4621 acgctgcgtg atgacggtgg tgatatggcg gatggtcttt acccggttct ttcctggctg
     4681 acctggccga tgtctgtggg taaatggacg cttgaaggga tcgaaacccg cgctcagctg
     4741 ctggattccg atggtctgct gcgtcagtcg tccgatcctt atattatggt gcgcgaagcg
     4801 tacttccagc gtcatgattt catcgctaat ggcggcgaac tcaaaccgca ggaaaatccg
     4861 aacgcacaag cgattcagga tgatttaaaa gatattgatt ctgaataaga aacaaataaa
     4921 aaaggtgagt cgcaaaactc accttttttg ttgttatccc tcaattacca caagtaaaag
     4981 gagataaacc gacaatgacc cggacgtaat gatgtaaaaa tactga
//
LOCUS       DADMKF010000218         4980 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.222, whole genome shotgun sequence.
ACCESSION   DADMKF010000218 DADMKF010000000
VERSION     DADMKF010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4980
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.222"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            274..1218
                     /gene="leuO"
                     /locus_tag="JNC24_003836"
     CDS             274..1218
                     /gene="leuO"
                     /locus_tag="JNC24_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HAY9160471.1"
                     /translation="MPEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
     gene            1536..3260
                     /gene="ilvI"
                     /locus_tag="JNC24_003837"
     CDS             1536..3260
                     /gene="ilvI"
                     /locus_tag="JNC24_003837"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025294.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HAY9160472.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVWPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     TAGCHQQLKETVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRARQCLKYDTHSEKIKPQAVIETLWRLTKGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVHNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            3263..3754
                     /gene="ilvN"
                     /locus_tag="JNC24_003838"
     CDS             3263..3754
                     /gene="ilvN"
                     /locus_tag="JNC24_003838"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HAY9160473.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVASTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIREVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            3766..3852
                     /locus_tag="JNC24_003839"
     CDS             3766..3852
                     /locus_tag="JNC24_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HAY9160474.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            3934..4938
                     /gene="cra"
                     /locus_tag="JNC24_003840"
     CDS             3934..4938
                     /gene="cra"
                     /locus_tag="JNC24_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HAY9160475.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREVAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
BASE COUNT         1212 a         1125 c         1310 g         1333 t
ORIGIN      
        1 acatttaatc cattttgtag atgattgagt attcgcggta gttatgatta gattgttttc
       61 gcaacaaaaa cattatggat tcttatgctg tggtaaatga ctcattccac ggcaatggat
      121 tctgttttta tcagaacccg tatctttatg ttttccgaat tttactcatt ttgctttttc
      181 ttattttata tgcatgataa atcatattct tcaggattat ttctctgcat tccaataagg
      241 gaaagggagt taagtgtgac agtggagtta agtatgccag aggtacaaac agatcattca
      301 gagacggcgg agttaagcaa gccacagcta cgcatggtcg atctcaactt attaaccgtt
      361 ttcgatgccg tgatgcagga gcaaaacatt acccgtgccg ctcatgttct gggtatgtcg
      421 caacctgcgg tcagtaacgc tgttgcacgc ctgaaggtga tgtttaatga cgagcttttt
      481 gttcgttatg gccgtggtat tcaaccgact gctcgcgcat ttcaactttt tggctcagtt
      541 cgtcaggcat tgcaactagt acaaaatgaa ttgcctggtt caggttttga acccgcgagc
      601 agtgaacgtg tatttcatct ttgtgtttgc agcccgttag acagcattct gacctcgcag
      661 atttataatc acattgagca gattgcgcca aatatacatg ttatgttcaa gtcttcatta
      721 aatcagaaca ctgaacatca gctgcgttat caggaaacgg agtttgtgat tagttatgaa
      781 gacttccatc gtcctgaatt taccagcgtg ccattattta aagatgaaat ggtgctggta
      841 gccagcaaaa atcatccaac aattaagggc ccgttactga aacatgatgt ttataacgaa
      901 caacatgcgg cggtttcgct cgatcgtttc gcgtcattta gtcaaccttg gtatgacacg
      961 gtagataagc aagccagtat cgcgtatcag ggcatggcaa tgatgagcgt acttagcgtg
     1021 gtgtcgcaaa cgcatttggt cgctattgcg ccgcgttggc tggctgaaga gttcgctgaa
     1081 tccttagaat tacaggtatt accgctgccg ttaaaacaaa atagcagaac ctgttatctc
     1141 tcctggcatg aagctgccgg gcgcgataaa ggccatcagt ggatggaaga gcaattagtc
     1201 tcaatttgca aacgctaact gaatgcagaa taggtcagac atgaatgtct ggtttattct
     1261 gtatttttta ttgaatgtag aattttattc tgaatgtgtg ggctctctat tttaggatta
     1321 attaaaaaaa tagagaaatt gctgtaagtt gtgggattca gccgatttat tatcaattta
     1381 atccttcgta atggaggatt ttatcgtttc ttttcacctt tcctcctgtt tattcttatt
     1441 accctgcgtt tatgtctctg gctgccaatt gcttaagcaa gatcggacgg ttaatgtgtt
     1501 ttacacattt tttccgtcaa acagtgaggc aggccatgga gatgttgtct ggagccgaga
     1561 tggtcgtccg atcgcttatc gatcagggcg ttaaacaagt attcggttat cccggaggcg
     1621 cagtccttga tatttacgat gcattgcata ccgtgggtgg tatcgatcat gttttagttc
     1681 gtcatgagca ggcggcagtg catatggccg atggtctggc gcgcgcgacc ggggaagtcg
     1741 gcgtcgtgct ggtaacgtcg ggtccagggg cgaccaatgc gattactggc atcgccaccg
     1801 cttatatgga ttccattcca ttagttgtcc tttccgggca ggtagcgacc tcgttgatag
     1861 gttacgatgc ctttcaggag tgcgacatgg tggggatttc gcgaccagtg gttaaacaca
     1921 gttttctggt taagcaaacg gaagacattc cgcaggtgct gaaaaaggct ttctggctgg
     1981 cggcaagtgg tcgtcctgga ccagtagtcg ttgatttacc gaaagatatt cttaatccgg
     2041 cgaacaaatt accctatgtc tggccggagt cggtcagtat gcgttcttac aatcccacta
     2101 ctaccggaca taaagggcaa attaagcgcg ctctgcaaac gctggtagcg gcaaaaaaac
     2161 cggttgtcta cgtaggcggt ggggcaatca cggcgggctg ccatcagcag ttgaaagaaa
     2221 cggtggaggc gttgaatctg cccgttgttt cctcattgat ggggctggga gcgtttccgg
     2281 caacgcatcg tcaggcactg ggcatgctgg gaatgcacgg tacctacgaa gccaatatga
     2341 cgatgcataa cgcggatgtg attttcgccg tcggggtacg atttgatgac cgaacgacga
     2401 acaatctggc aaagtactgc ccaaatgcca ctgttctgca tatcgatatt gatcctactt
     2461 ccatttctaa aaccgtgact gcggatatcc cgattgtggg ggatgctcgc caggtcctcg
     2521 aacaaatgct tgaactcttg tcgcaagaat ccgcccatca accactggat gagatccgcg
     2581 actggtggca gcaaattgaa cagtggcgcg ctcgtcagtg cctgaaatat gacactcaca
     2641 gtgaaaagat taaaccgcag gcggtgatcg agactctttg gcggttgacg aagggagacg
     2701 cttacgtgac gtccgatgtc gggcagcacc agatgtttgc tgcactttat tatccattcg
     2761 acaaaccgcg tcgctggatc aattccggtg gcctcggcac gatgggtttt ggtttacctg
     2821 cggcactggg cgtcaaaatg gcgttgccag aagaaaccgt ggtttgcgtc actggcgacg
     2881 gcagtattca gatgaacatc caggaactgt ctaccgcgtt gcaatacgag ttgcccgtac
     2941 tggtggtgaa tctcaataac cgctatctgg ggatggtgaa gcagtggcag gacatgatct
     3001 attccggccg tcattcacaa tcttatatgc aatcgctacc cgatttcgtc cgtctggcgg
     3061 aagcctatgg tcacgtcggg atccagattt ctcatccgca tgagctggaa agcaaactta
     3121 gcgaggcgct ggaacaggtg cacaataatc gcctggtgtt tgttgatgtt accgtcgatg
     3181 gcagcgagca cgtctacccg atgcagattc gcgggggcgg aatggatgaa atgtggttaa
     3241 gcaaaacgga gagaacctga ttatgcgccg gatattatca gtcttactcg aaaatgaatc
     3301 aggcgcgtta tcccgcgtga ttggcctttt ttcccagcgt ggctacaaca ttgaaagcct
     3361 gaccgttgcg tcaaccgatg atccgacatt atcgcgtatg accatccaga ccgtgggcga
     3421 tgaaaaagta cttgagcaga tcgaaaagca attacacaag ctggtcgatg tcttgcgcgt
     3481 gagtgagttg gggcagggcg cgcatgttga gcgggaaatc atgctggtga aaattcaggc
     3541 cagcggttac gggcgtgacg aagtgaaacg taatacggaa atattccgtg ggcaaattat
     3601 cgatgtcaca ccctcgcttt ataccgttca attagcaggc accagcgata agcttgatgc
     3661 atttttagca tcgattcgcg aggtggcgaa aattgtggaa gttgctcgtt ctggtgtggt
     3721 cggactttcg cgcggcgata aaataatgcg ttgagaatga tctgaatgcg caatttgcag
     3781 cccaacatgt cacgttgggc tttttttgcg aaatcagtgg gaacctggaa taaaagcagt
     3841 tgccgcagtt aattttatgc gcttagatgt taatgaattt aacccatacc agtacaatgg
     3901 ctatggtttt tacattttac gcaaggggca attgtgaaac tggatgaaat cgctcggctg
     3961 gcgggagtgt cgcggaccac tgcaagctat gttattaacg gcaaagcgaa gcaataccgt
     4021 gtgagcgaca aaaccgttga aaaagtcatg gctgtggtgc gtgagcacaa ttaccacccg
     4081 aacgccgtgg cagctgggct tcgtgctgga cgcacacgtt ctattggtct tgtgatcccc
     4141 gatctggaga acaccagcta tacccgcatc gctaactatc ttgaacgcca ggcgcggcaa
     4201 cggggttatc aactgctaat tgcctgctca gaagatcagc cagacaacga aatgcgctgt
     4261 attgagcacc ttttacagcg tcaggtcgat gccattattg tttcgacgtc gttgcctcct
     4321 gagcatcctt tttatcaacg ctgggctaac gacccgttcc cgattgtcgc gctggaccgc
     4381 gccctcgatc gtgaacactt caccagcgtg gttggtgccg atcaggatga tgccgaaatg
     4441 ctggcggaag agttacgtaa gtttcccgcc gagacggtgc tttatcttgg cgcgctgccg
     4501 gagctttctg tcagcttcct gcgtgaacaa ggtttccgta ctgcctggaa agatgatccg
     4561 cgcgaagtgc atttcctgta tgccaacagc tatgagcggg aggtggctgc ccagttattc
     4621 gaaaaatggc tggaaacgca tccgatgccg caggcgctgt tcacaacgtc gtttgcgttg
     4681 ttgcaagggg tgatggatgt cacgctacgt cgcgatggta aactgccttc tgacctggca
     4741 attgccacct ttggcgataa cgaactgctc gacttcttac agtgcccggt gctggcagtg
     4801 gctcaacgtc accgcgatgt cgcagagcgt gtgctggaga ttgtcctggc aagcctggac
     4861 gaaccgcgta agccaaaacc tggtttaacg cgcattaaac gtaatctcta tcgccgcggt
     4921 gtgctcagcc gtagctaagc cgcgaacaaa aatacgcgcc aggtgaattt ccctctggcg
//
LOCUS       DADMKF010000219         4814 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.223, whole genome shotgun sequence.
ACCESSION   DADMKF010000219 DADMKF010000000
VERSION     DADMKF010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4814
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.223"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            197..1171
                     /gene="hemB"
                     /locus_tag="JNC24_003841"
     CDS             197..1171
                     /gene="hemB"
                     /locus_tag="JNC24_003841"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HAY9160476.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
     gene            complement(1277..2128)
                     /gene="tauD"
                     /locus_tag="JNC24_003842"
     CDS             complement(1277..2128)
                     /gene="tauD"
                     /locus_tag="JNC24_003842"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HAY9160477.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRYQV
                     VFLRDQAITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSGLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(2125..2952)
                     /gene="tauC"
                     /locus_tag="JNC24_003843"
     CDS             complement(2125..2952)
                     /gene="tauC"
                     /locus_tag="JNC24_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HAY9160478.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVVIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     VLAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            complement(2949..3716)
                     /gene="tauB"
                     /locus_tag="JNC24_003844"
     CDS             complement(2949..3716)
                     /gene="tauB"
                     /locus_tag="JNC24_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HAY9160479.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSGGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSSGPGRVLERLPLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            complement(3729..4691)
                     /gene="tauA"
                     /locus_tag="JNC24_003845"
     CDS             complement(3729..4691)
                     /gene="tauA"
                     /locus_tag="JNC24_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY9160480.1"
                     /translation="MAISSRNTLLAALAFIAFQAQAVNVTVAYQTSAEPAKVAQADNT
                     FAKESGASVDWRKFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKL
                     GNSEALVVKKTISKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGQVEIVNLQPPA
                     IIAAWQRGDIDGAYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHP
                     EVVKAFAKSAIDAQQPYIANPDAWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQ
                     QQTAELTGPVNKAIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
BASE COUNT         1115 a         1391 c         1231 g         1077 t
ORIGIN      
        1 aaaataataa ccaagagcta taaataaaga ttaaaaatat tttcagtgaa tatgaaatta
       61 ttgctatttc tattacaaag atgtcctggt ctctctttca ttctcttctt gtcaacatca
      121 cgacaacttt cgtaaaacac tcctgccccg cttcaggtat actgtgcacc ttaattccac
      181 aaacatcagg cagaccatga cagacttaat ccaacgccct cgtcgcctac gcaaatctcc
      241 tgcgctgcgc gctatgtttg aagagacaac acttagcctt aacgacctgg tgttgccgat
      301 ctttgttgaa gaagaaattg acgactacaa agccgttgaa gccatgccag gcgtgatgcg
      361 cattccagag aaacatctgg cacgcgaaat tgaacgcatc gccaacgccg gtatccgttc
      421 cgtaatgact tttggcatct ctcaccatac cgatgaaacc ggcagcgacg cctggcggga
      481 agatggactg gtggcgcgta tgtcgcgcat ctgcaagcag actgtgccag aaatgatcgt
      541 catgtcagac acttgtttct gtgaatacac ttctcacggt cactgcggtg tgctgtgcga
      601 gcatggcgtc gacaacgacg cgactctgga aaatttaggc aagcaagccg tggttgcagc
      661 tgctgcaggc gcagacttca tcgccccttc cgccgcgatg gacggccagg tacaggcgat
      721 tcgtcaggcg ctggacgctg caggctttaa agatacggcg attatgtcgt attcgaccaa
      781 gttcgcctct tccttttatg gcccgttccg tgaagctgcc ggaagcgcat taaaaggcga
      841 ccgcaaaagc tatcagatga acccaatgaa ccgtcgcgag gcgattcgtg aatcactgct
      901 ggatgaagcc cagggcgcag actgcctgat ggttaaacct gctggagcgt acctcgacat
      961 cgtgcgtgag ctgcgtgaac gtactgaatt gccgattggc gcgtatcagg tgagcggtga
     1021 gtacgcgatg attaagttcg ccgcgctggc gggtgctata gatgaagaga aagtcgtgct
     1081 cgaaagctta ggttcaatta agcgtgcggg tgcggatctg attttcagct actttgcgct
     1141 ggatttggct gagaagaaga ttctgcgtta atttttttcc tctcagaaag ggggagttaa
     1201 cctgttgaaa gtacggtgaa cgccccctca ccctaaccct ctccccaagg gggcgaggga
     1261 accgtccgca ctcgtatcac cccgcccgat aaaacggttt atccccaagg atcgtcgccc
     1321 gatgcataat ccgccgctgt ggcaggtaat cggcattggc atagtgctga gtcacgcggt
     1381 tatcccaaat cgcaatatca ttcggttgcc agcgccaacg cacctgaaac tccggtttag
     1441 tgatatgggc gaataaaaag cctaacaagg cttcgctctc tttctcgctg acatcgacaa
     1501 ttcgcgtagt aaagccttca ttcacaaaca gcgcctgttt accgctcacc ggatgcgttc
     1561 gcaccaccgg atgtagcaac ggaggatttt tcgcgaccgc ctcgcgccag cgttgatgtt
     1621 cctcctcggt tttgcggtat ttgtattccg ggaacgattt acggaaatca tgctccgcac
     1681 gcagcccgct cagcagctgg cggaagggaa cagagagcgc ctcataggcc gcaataccgc
     1741 tggtccagag cgtatcaccg ccggtcgaag gtaactcttt agctgccaga atcgcccctg
     1801 cgggtggcgt ttcaataaat gtcacatcgg tatgccagtt gtcgttatcc ggcggattat
     1861 cgttatgggt atccagcacg atgatctcgt caaccccttc ggcatgaggg taaacagggt
     1921 gaatatgcag ttcgccaaaa cgctgggcca gcgcgcgttg ctgctgcggc gtaatagctt
     1981 gatcgcgcag aaataccacc tgatagcgca gcaccgcatg gtaaagctgt tcaaactgat
     2041 tatcgcttaa cgggcgcgtc aggtcggcac ccgaaatttg tgcgccgata tacggcccca
     2101 gcggggtaat actcagacgt tcactcattg tacttctcca tgccagggcg tcagacggcg
     2161 ctgtaacgcg cgcagaccca gttctaaaag aaaggcgata atcgcaatca ccgcgatccc
     2221 cgccagtacc acgtcagtcg cgagaaattc acccgctgac tgaaccataa atcctaaacc
     2281 gcgtgtcgcg gcaatcagct ccgccgctac cagcgtagac cagcccacac ccagaccaat
     2341 gcgtaatccg gtgaggattt ccggcagcgc gccgggcaaa atgacaaacc acagcacctg
     2401 cgcacggctg gcacccagcg actgggcggc acgaatgcga acctgctgcg cgcttttcac
     2461 ccccgccagc accgacatcg ccaccggtgc aaaaatcgct aaatagatca ataagatctt
     2521 cgaggtttca ccaataccaa accagatcac catcagcggc aaataagcca gcggcggcac
     2581 cggacgataa agttcgatta ccggatccag aatgccgcgc accgtcgggc ttagccccat
     2641 ggcgatcccg accggaatac cgatcaccac cgccgcgagt agcgccagca caatgcgcgt
     2701 caggctggct gccagatgct gccacagcgt ggcgtccata aatccctgcg gtccggcaat
     2761 ggtgagcagt tttgccagta cttgttgcgg cggcggcaaa aacagcgggc taatcagttg
     2821 cagcgccgcc accgtccacc atacagtgag taaaaccgct aacgtgccaa tgcttaaggt
     2881 cacctgacgc gagagcggcc agcgccattt cagccgccgc gaatgcagtt tttcattaat
     2941 gagcacactc atgagaacgc ctcccgttgc tcaaatacgc ggcttaaaac atattcgcgc
     3001 atggcgatga attgtggatc ggacttgatg ctgcggctcg actctcccgc aacaaagcgg
     3061 cgagcaaagt tgagcggcag ccgctccagc acacggccag ggccggatga aagcagaacc
     3121 agttcagtcg ccataaacac cgcttcttct atatcgtggg taatcaacag cacctgcttg
     3181 cccgtctcct gccagagttt cagcagcaga gtttgcatct ggtcgcgggt gaaggcgtcc
     3241 agcgcaccaa acggttcgtc gagtaataac agctggggat tcgccgccag cgcgcgggca
     3301 atccccaccc gctgacgttg accaccggaa agctgccaga tatagcgttt ttctgcgcct
     3361 tccagcccca cttttttcag catctggtgc gcgatttcca gtcgctgcat tttctctata
     3421 cctgccaatt gcaggccgaa cgccacgttg tcctgtacat tgcgccacgg tagtagccct
     3481 tcattctgaa aaaccacccc gcgctctgct cccggtccct caatacgctt acccgccagt
     3541 tgaatgctgc catgctgata aggcacaaaa ccggcaatca gattcagcag ggtggtttta
     3601 ccgcagccgg acggccccag caccaccagt agctcgccgc tttccagcgt caggttgata
     3661 tcttccagtg ccggtttgcc gccataatcg gcatagagat gagagatttg cagcatctgc
     3721 gcctcctttt attgcacgaa gcgcgaggta acgtactggc tgtaatcatt cgctacagcc
     3781 gggaccttgc cctgctcttt caaaaactgc gcggtgtcga tgattgcttt gttcaccggt
     3841 ccggtcagtt ctgccgtttg ttgctgcggc gtcagatagg tattcccctt caccagcccc
     3901 ggaacgtcac cttccggcac gccgcttaaa cgcgccagtt tgctgatgtt ttccggctgt
     3961 ttcagccacg cgtctgggtt agcaatgtac ggttgctgag catcgatggc gcttttagcg
     4021 aacgctttca cgacctcagg atgtttctcg gcaaaatctt tgcgcaccac ccagacgtcc
     4081 agcgttggcg caccccactg cccgacctgt tcagaatcgg tcaacacctt gccgtctttt
     4141 tccagggcgt taaccgccgg tgcccagaca taagcaccat caatatcacc ccgctgccat
     4201 gcggcgataa tcgcgggcgg ctgcaggttc acaatctcca cttgccctgg tttaatcccc
     4261 cagtgtttca gcgccgccag caggctgtag tgggtggtgg agataaacgg tacggcaatg
     4321 cgtttgccaa tcaaatcttc gggtttgctg atagttttct ttaccaccag cgcttcggag
     4381 ttacccagtt ttgacgccag caagaagact tcaatcggca cctgttggct ggctgcaacc
     4441 gctaatgggc tggaaccgag gttgccgatt tgcacgtcgc cagaagccag cgcccgcacg
     4501 atgctggctc cgctgtcaaa cttacgccag tccacgctcg ccccgctctc tttagcaaag
     4561 gtgttgtcgg cctgagccac tttcgccggt tcggctgagg tttgatacgc cacggtgacg
     4621 ttcaccgcct gtgcctgaaa agcgatgaat gccagtgcgg caagaagtgt gttacgcgat
     4681 gaaattgcca tgattgtctg ctcccctgtc ttgttatggg agcagtattc aggaataaaa
     4741 acattcatta aaagaattag tcgttatcgc acagatgatt ttattcttag caaaaaaacg
     4801 gcaaaggaaa gatt
//
LOCUS       DADMKF010000220         4799 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.224, whole genome shotgun sequence.
ACCESSION   DADMKF010000220 DADMKF010000000
VERSION     DADMKF010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4799
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.224"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            241..582
                     /gene="raiA"
                     /locus_tag="JNC24_003846"
     CDS             241..582
                     /gene="raiA"
                     /locus_tag="JNC24_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HAY9160481.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     gene            686..733
                     /locus_tag="JNC24_003847"
     CDS             686..733
                     /locus_tag="JNC24_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9160482.1"
                     /translation="MKHTPFFFAFFFTFP"
     gene            833..1993
                     /gene="pheA"
                     /locus_tag="JNC24_003848"
     CDS             833..1993
                     /gene="pheA"
                     /locus_tag="JNC24_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HAY9160483.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQQQN
                     FTRFVVLARKAINMSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEIFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(2036..3157)
                     /gene="tyrA"
                     /locus_tag="JNC24_003849"
     CDS             complement(2036..3157)
                     /gene="tyrA"
                     /locus_tag="JNC24_003849"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HAY9160484.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(3168..4238)
                     /gene="aroF"
                     /locus_tag="JNC24_003850"
     CDS             complement(3168..4238)
                     /gene="aroF"
                     /locus_tag="JNC24_003850"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HAY9160485.1"
                     /translation="MQKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHRFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            4447..>4799
                     /locus_tag="JNC24_003851"
     CDS             4447..>4799
                     /locus_tag="JNC24_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HAY9160486.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVESASQ
                     LHEVWQKGADENAST"
BASE COUNT         1311 a         1229 c         1096 g         1163 t
ORIGIN      
        1 tgagctgccg tttttttatt ctgttagttg tgaaactgaa gcgatttagt cactatcgat
       61 ctcatcaaat atggctcgct ttgagatatt cctcaagtaa aaaaacacct cttcctgcga
      121 tttctcacaa aaaagattcg ttgacaaaaa gtgacaaaat tatgagattc ccatcacaca
      181 ttttgacatc aggaacggta tgctgaattc accaagacgg gaagacaaga ggtaaaattt
      241 atgacaatga acattaccag caaacaaatg gaaattactc cggccatccg ccaacatgtc
      301 gcagaccgtc tcgccaaact ggaaaaatgg caaacacatc tgattaatcc acatatcatt
      361 ctgtccaaag agccacaagg gtttgttgct gacgccacaa tcaatacacc taacggcgtt
      421 ctggttgcca gtggtaaaca tgaagatatg tacactgcaa ttaacgaatt gatcaacaag
      481 ctggaacggc agctcaataa actgcagcac aaaggcgaag cacgtcgtgc cgcaacatcg
      541 gtgaaagacg ccaacttcgt cgaagaagtt gaagaagagt agtcctttat attgagtgga
      601 tcgccaacgc gccttcgggc gcgttttttg ttgacagcgt gaaaacagta cgggtactgt
      661 actaaagtca cttaaggaaa caaacatgaa acacacaccg tttttcttcg cattcttttt
      721 taccttcccc tgaatgggag gcgtttcgtc gtgtgaaaca gaatgcgaag acgaacaata
      781 aggcctccca aaccgggggg ccttttttat tgataacaaa aaaggcaaca ctatgacatc
      841 ggaaaatcca ttactggcgc tgcgagagaa aatcagcgcg ctggatgaaa aattattagc
      901 attactggca gagcggcgcg aactggccgt cgaggtggga aaagccaaac tgctctcgca
      961 tcgcccggta cgagatattg atcgtgaacg cgatttactg gaaagattaa ttacgctcgg
     1021 taaagcgcac catctggacg cccattacat tactcgcctg ttccagctca tcattgaaga
     1081 ttccgtatta actcagcagg ctttgctcca gcaacatctc aataaaatta atccgcactc
     1141 agcacgcatc gcttttctcg gccccaaagg ttcttattcc catcttgcgg cgcgccagta
     1201 tgctgcccgt cactttgagc aattcattga aagtggctgc gccaaatttg ccgatatttt
     1261 taatcaggtg gaaaccggcc aggccgacta tgccgtcgta ccgattgaaa ataccagctc
     1321 cggtgccata aacgacgttt acgatctgct gcaacatacc agcttgtcga ttgttggcga
     1381 gatgacgtta actatcgacc attgtttgtt ggtctccggc acgactgatt tatccaccat
     1441 taatacggtc tacagccatc cgcagccatt ccagcaatgc agcaaattcc ttaatcgtta
     1501 tcctcactgg aagattgaat ataccgaaag tacgtctgcg gcaatggaaa aggttgcaca
     1561 ggcaaaatca ccgcatgttg ctgcgttggg aagcgaagct ggcggcactt tgtacggttt
     1621 gcaggtactg gagcgtattg aagcgaatca gcaacaaaac ttcacccgat ttgtggtgtt
     1681 ggcacgtaaa gccattaaca tgtctgacca ggttccggcg aaaacgacgt tgttaatggc
     1741 gaccgggcaa caagccggtg cgctggttga agcgttgctg gtgctgcgca accacaatct
     1801 gattatgacc cgtctggaat cacgcccgat tcacggtaat ccatgggaag agatatttta
     1861 tctggatatt caggccaatc ttgaatcagc ggaaatgcaa aaagcattga aagagttagg
     1921 ggaaattacc cgttcaatga aggtattggg ctgttaccca agtgagaacg tagtgcctgt
     1981 tgatccaacc tgatgaaaag gtgccggatg atgtgaatca tccggcactg gattattact
     2041 ggcggttgtc attcgcctga cgcaataaca cgcggctttc actctgaaaa cgctgtgcgt
     2101 aatcgccgaa ccagtgctcc accttgcgga aactgtcaat aaacgcctgc ttatcgccct
     2161 gctccagcaa ctcaatcgcc tcgccgaaac gcttatagta acgtttgatt aacgccagat
     2221 tacgctctga cgacataata atgtcggcat aaagctgcgg atcctgagca aacagtcgcc
     2281 cgaccatcgc cagctcaagg cggtaaatcg gcgaagagag cgccagaagt tgctcaagct
     2341 gaacattttc ttctgccaga tgcagcccat aagcaaaagt agcaaagtgg cgcagagcct
     2401 gaataaacgc catattctga tcgtgctcga cagcgctaat acgatgcagc cgagcgcccc
     2461 agacctgaat ttgctccaga aaccattggt atgcttccgg cttacgtcca tcacaccaga
     2521 ccacaacttg ctttgccagg ctaccgctgt ccgggccgaa catcgggtgt aaccccagta
     2581 ccgggccatc gtgcgccgcc agcatggcct gtaatggtcc atttttcact gatgccagat
     2641 caaccagaat acaatctttc ggtaaaggcg gtaatttgcc gataacttgc tcagtaacgt
     2701 ggattggcac actaacaatc accattccgg catcggcaac aatatcagcc gctcgatccc
     2761 agtcatgttg ctccagaatc cgcacctgat aacccgagag tgtcagcatc ttctcgaaca
     2821 ggcgtcccat ctgaccgcca ccgccgacga taaccaccgg acgcagtgac ggacaaagtg
     2881 ttttaaatcc tttgtcgttt tcactggagt aagattcacg catcacccga cgcaaaacat
     2941 cctcaatcag atctggcggt acacccagag cttccgcctc tgcacgacgc gaggccaaca
     3001 tagatgcctc gcgctccgga acataaatag gcagtccaaa gcggcttttc acctcgccca
     3061 cttcagcaac cagttccaga cgcttcgcta ataaattcag cagcgcttta tcgacttcat
     3121 caatttgatc gcgtaatgcg gtcaattcag caaccataat aaacctctta agccacgcga
     3181 gccgtcagct gcccgttcag atcctgatga atttcacgca gcaaggcatc ggtcatttcc
     3241 cagctaatgc aggcatcggt tacggataca ccgtatttca tttcactgcg cggttgctca
     3301 gaagactgat tgccctcgtg gatattactt tcgatcatca gaccaataat tgagcgattg
     3361 ccatctttga tttgagcaac cacggattct gccaccgcag gctgacggcg ataatcttta
     3421 ttggaattac cgtggctgca atctaccatc agagacgggc gcagtcccgc ctgttccatc
     3481 tctttttcac attgcgcaac atccgcaggg ctatagttcg gcgctttacc accgcgcagg
     3541 atgacatgcc cgtccggatt cccctgagtt tgtagcaacg caacctgccc tgcctggtta
     3601 atgccaacaa aacggtgcgg ctgggcggcg gcgcgcatag cgttaattgc tgttgccaga
     3661 ctgccgtcgg tgccgttttt aaaaccaacc ggcatggaaa gcccggaggc catttcacgg
     3721 tgagtttgcg attccgttgt acgagcacca attgctgacc agctaaacag atcgcccagg
     3781 tattgcgggc tattcggatc taacgcttcc gtcgccagtg gcagtcccat attcaccagc
     3841 tcaagcagca atttacgcgc gatctgcagc ccggcttcta catcaaaaga gccatccata
     3901 tggggatcgt taattaaccc tttccagccg acagtggtac ggggtttttc aaaatagacg
     3961 cgcattacca gatagaggct atcgctgacc tctgcggcaa gggctttaaa tcgacgagca
     4021 tattccagag cagtttccgg atcatgaatg gaacaaggac cacaaactac cagcagacga
     4081 ggatcgcgcc cggcgataat atctgaaatg gttttacgcg agtcagcaat ctgggcttct
     4141 tgttgcaggc tcaatggaaa agcggccttc agttgttccg gagtcattaa aacctgttcg
     4201 tcggtaatat gtacgttatt cagcgcgtct ttttgcatga tggcgatcct gtttatgctc
     4261 gtttgcgata gttgatcctc agcgaggatg acgtaacgat aacacataaa gtaaagtttt
     4321 caatccatat ttcgtacatt tttctttaca cgaacaattt gctcgcgctt ttgatgctta
     4381 tttcgtacaa ataaatttac acctccttta aaaacaatcc gctatgcttt gaaaaaggag
     4441 aaagaaatga tgaaaaagtt tatcgccccc ttattggctt tactggttag cggatgtcag
     4501 attgatcctt atactcacgc gccaaccttg accagcaccg actggtatga tgtcggtatg
     4561 gaagatgcga tatcgggcag cgccataaaa gatgacgatg catttagcga ttcacaggcg
     4621 gatcgcggtc tataccttaa aggatatgcc gaaggacaaa agaaaacttg ccagaccgat
     4681 tttacttatg cccggggact ttccggtaaa agctttcctg ccagctgtaa caatgttgaa
     4741 agtgccagcc aactgcatga agtatggcaa aaaggggctg atgaaaatgc cagcacaat
//
LOCUS       DADMKF010000221         4796 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.225, whole genome shotgun sequence.
ACCESSION   DADMKF010000221 DADMKF010000000
VERSION     DADMKF010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4796
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.225"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            6..81
                     /locus_tag="JNC24_003852"
     tRNA            6..81
                     /locus_tag="JNC24_003852"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39..41,aa:Thr,seq:ggt)
     gene            121..236
                     /gene="rrf"
                     /locus_tag="JNC24_003853"
     rRNA            121..236
                     /gene="rrf"
                     /locus_tag="JNC24_003853"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(299..1057)
                     /locus_tag="JNC24_003854"
     CDS             complement(299..1057)
                     /locus_tag="JNC24_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAY9160487.1"
                     /translation="MSQILLQPANAMITLENVNKWYGQFHVLKNINLTVQLGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQAAPDEFFAHPKSERTKAFLSQVIH"
     gene            complement(1065..2168)
                     /locus_tag="JNC24_003855"
     CDS             complement(1065..2168)
                     /locus_tag="JNC24_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAY9160488.1"
                     /translation="MTKVLLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAVWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTNIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(2178..3359)
                     /locus_tag="JNC24_003856"
                     /pseudo
     CDS             complement(2178..3359)
                     /locus_tag="JNC24_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026209.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
     gene            complement(3427..4452)
                     /locus_tag="JNC24_003857"
     CDS             complement(3427..4452)
                     /locus_tag="JNC24_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY9160489.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPGFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTDYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVGNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
BASE COUNT         1315 a         1238 c         1146 g         1097 t
ORIGIN      
        1 agcgtgctga tatggctcag ttggtagagc gcacccttgg taagggtgag gtccccagtt
       61 cgactctggg tatcagcacc actttttagg ttaaagttcg gcagattaga aaagaatttg
      121 tctggcggca gtagcgcggt ggtcccacct gaccccatgc cgaactcaga agtgaaacgc
      181 cgtagcgccg atggtagtgt agggtctccc catgcgagag tagggaactg ccagacatca
      241 aatcaagcga aaggccatcc gaaaggatgg cctttttgct tttcgaacta acattcaatt
      301 aatggattac ctgcgataaa aatgccttcg tacgctctga tttaggatgc gcaaaaaatt
      361 catcaggtgc agcttgctcc actatttccc cacgatccat aaaaatcacc cggtcagcga
      421 cggttcgcgc aaaccccatc tcatgcgtta cacacaacat tgtcataccc gattgcgcca
      481 gcccaatcat cgtatccagc acctctttca ccatctccgg atcgagcgcc gacgttggct
      541 catcaaacaa cataattttc ggcttcatac acagcgaacg cgcaatggca acgcgttgct
      601 gctgaccacc agaaatttgt ccgggaaact tatgcgcatg ttcggcaatt ctcacccgct
      661 caaggtaatg catcgccaga gcttcagcct ctttctttgg catcttgcgt acccaaatcg
      721 gtgccagggt acagttctgt agaacggtca gatgagggaa gagattgaaa tgctgaaaga
      781 ccattcccac ttcctgcctg acgcgctcaa tattgcggat atcttcatta agttcgatgc
      841 catctactac gatccgtccc tgttgatgtt cttccagatg attaatacaa cgaatggttg
      901 tcgatttacc ggaaccagaa gggccacaca gaacgatccg ttctcccagt tgcacggtta
      961 aatttatatt tttcaaaaca tggaattgtc cataccattt attgacgttt tccagcgtaa
     1021 tcatcgcgtt agcaggttgc agtaaaattt ggctcatagt gtcctcaatg cggtgtacgc
     1081 ccggtgttaa aacgtttttc cagatgctgg ctatagcgcg acatgctgaa acagaagatc
     1141 cagtagatca gtgcggcgaa aacataccct tccgtcgaca tacccaacca ggcgggatca
     1201 acggttgcct gctgaacgct actgaaaaga tcgaacaacc cgataatgat caccaggctg
     1261 gtatctttga agagtgcgat gatggtattt accagcccag gaattaccag cttcaacgcc
     1321 tgtggcagaa taaccagccc ctgagttttc cagtaaccca atgccagtga ctctgccgct
     1381 tcatattgcc ctttaggcag cgcctgtaat ccgcctcgca cgacttccgc aacatatgct
     1441 gactgaaaca ggatcacgcc aaccagcgcg cggatcagtt tgtcgatatt ggtgccttct
     1501 gccataaaca aaggcagcat gaccgaagac ataaacaata cggtaatcag cggtacgccg
     1561 cgccagaact cgataaaaat gaccgataag atacgcacaa tcggcatatg ggagcggcga
     1621 cctaacgcca gtaagatccc ccacggtagc gcccccgcaa tcccaacgga tgcaataatt
     1681 aaagttagcg tcagcccgcc ccattgccgg gtttcaaccc gctccagacc aaaaaaaccg
     1741 ccatacatca gccaccagac aatcagtggg taaatcaccg cccagaccgc aatatagcga
     1801 ccgcgatgcg ggagtatttt ccagaacatt ggtgcgattg acacaaggcc aatcagtaat
     1861 gccaggttaa ttcgccagcg ttggtcgtgt gggtaaagtc catacataaa ctgaccaaat
     1921 cgttcgtgga tgaagaccca acaggcaccg gcttttgtgc agtcggcacg cgttgagcca
     1981 acccaattag cctgcaaaaa tgcccagttc agcaacggtg ggatcagttc ccacatcaac
     2041 catatgcagc caatagtcag caggctattg ctccagctgg agaacagatt ttttcgcacc
     2101 cacaccatcg cgcggcttga gttatggctc gctgggcgcg agggctgaga cagcaatact
     2161 tttgtcatca tgactcctta gcgttcaacg atcgcgatgc ggcggttata gatattcatc
     2221 agcagcgaga tagtcaggct gataatcaga tagaccgaca tggtcatggc tatcgtctcg
     2281 atggcttgcc ccgtctggtt cagcacggtg ccagcaaaca gcgaaaccat atcgggatag
     2341 ccaatagcgg cggcaagaga ggagtttttg acgatgttga gatactggct ggttaacggc
     2401 ggaataatca ctcgcagtgc ctggggaata atgacctagc gtagcgtaac cgggttgggt
     2461 aatcccagtg accgagccgc ttcatgttga ccataaggca ctgcctggat cccagcgcgg
     2521 ataatctcgg cgataaatgc agatgtataa accgaaagtg ccagcgttaa ggctgccagt
     2581 tcaggaatta aaaccatccc gccgcggaag ttaaagcctc gtagggctgg gacatcccag
     2641 tgtagtgctg cgccaaaaag ccattgcgcc agtaaaggca aaccaatgat caacactgcg
     2701 gcgatcggcc aggtgcggcg cagttgtccg gtctttatct ggtatgtttt attaaagcgg
     2761 aatagcccaa cagaaaggac tatagccata acaacagcca ggataaacgc aataaatccg
     2821 tctcccagct gcggtgacgg aatataaagc ccacgattgc tcaaaaaggc cagatcaaac
     2881 gcgctcacag cctggcgtgg tccgggcaaa ttgcgcaaca cggcaaagta ccagaagaag
     2941 atttgcagca gcgggggaat attacggaag atctcaatat aaattgtgga aagctttcgt
     3001 agcagccagt tatccgaaag tctcgccaga ccgataaaga agcccaaaac agaagcgaac
     3061 acaatacaca atgcagaaac cagtagcgta ttgagtaagc cgacaataaa aacgcgtccg
     3121 taggtgtcgc cctgctggta atcgatcaaa tgctggacaa taccgaagcc agcgccgcga
     3181 tccagaaagg caaaacctga agtaatgcca cgattattga gattcgttac agtgttgtga
     3241 aataaccaac caacaatgcc gacaacagca acaacggcaa ggatctggaa taaccaggca
     3301 cgaaccgtag ggttggcaaa agagagtgag cctttaacgg ttgagcggcg atgagacata
     3361 gaaacctcgg aaagcataac gctgaagaga gcgccgccgt agcgacgctc gttacctcac
     3421 accgacttaa cgcacgggcg gcgcgtactg aataccgccg ttattccaga gattattttg
     3481 cccacgttta attttcagcg ggctttctga acctacgtta cgctcgaaaa tttccgagta
     3541 gttacccacc tgtttgatga tgttataggc ccatttatta tccagcttca gatccttgcc
     3601 gtaatcgcct tctttaccca gcagatgtgc catatcaggc gttgctggat tagccgcttt
     3661 ttcatcgacg ttctgggaat tgatgcccat ctcttcagca ttcagcatgg cgaaaagcgt
     3721 ccagcgtaca atcgagaacc attcatcatc gccacgacga actaccggcc caagcggttc
     3781 tttagagata acctccggta agacaatcca ttcagctggg ttgcttaatt tgatgcgcag
     3841 ggcatacagt tgcgactgat ccgaggccag cgtatcgcag cgaccagatt ccagtgcctt
     3901 cgctgattcg tcagagcgat cgaaagtcac cggtgtgtac ttcatattgt ttgccttgaa
     3961 atagtcggcg acgttgagtt cggtatctgt acccgcctga atacagacgg tagcgccatc
     4021 cagttctttc gcgcttttta gccccgcttt atcgtgcgtc aggaagccaa tgccgtcgta
     4081 atcagtgacg ccagtaaatg ccattcccat cccggcatcg cgagatgaag tccaggtcgt
     4141 attacgggag agcaaatcca cctcccctga ctgtaaagcg gtgaagcgtt cttttgcagt
     4201 gagcggggta tatttcactt tcgtgtcgtc accaaataca gcagcggcaa caccacgaca
     4261 aacatctaca tcaatacctg aaaacttacc gtctgcatcg gcataagaga acccaggtaa
     4321 tccatcactg atcccgcatt gcacaaaacc ttttttctgt acggcatcaa gcgtcgcgcc
     4381 agcatgcgcc tgatttgcaa cggcaagcag cacgctggcg gcagccagtg tggctatcat
     4441 catctttttc ataatgcttc ctgtgaggcg aaaattatcg ttataggacg tttgggaaac
     4501 gtcttttacc tgtctgtggc catcctctta taagcaaaag tagtgccatc ttttgcgcac
     4561 gatttcgcgt cgcaataagc tgagtgtaga caggaaataa taaaaacagc gccccgcaat
     4621 ggtgcgaaaa tgaaacatgc gccacatttt ggcgcaaaat atttacgcgt gttttactaa
     4681 gcagaataat gattgttagc gtgagtatat ttctggtggg aggtgtgaaa gttaaattga
     4741 tgcgttattt cgaaagcagc gaaataacgc gatataacat gctaaggtaa tgactc
//
LOCUS       DADMKF010000222         4734 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.226, whole genome shotgun sequence.
ACCESSION   DADMKF010000222 DADMKF010000000
VERSION     DADMKF010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4734
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.226"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(<1..737)
                     /locus_tag="JNC24_003858"
     CDS             complement(<1..737)
                     /locus_tag="JNC24_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
                     /protein_id="HAY9160490.1"
                     /translation="MRHFIYQDEKSHKFWAVEQQGNELHISWGKVGTKGQSQIKSFSD
                     AAAAAKAELKLIAEKVKKGYVEQAKDNSLQPSQTVTGSLKVADLSTIIQEQPSFVAET
                     RAPDKNTDAVLPWLAKDIAVVFPPEVVHTTLSHRRFPGVPVQQADKLTQLRRLACSVS
                     QRDNKTATFDFSACSLEWQNTVAQAISQIDGLKTTQLPSPVMAVLTALEMKCTRYKVR
                     EDVMDQIVQEGGLEYATDVIIHLQQID"
     gene            complement(878..2911)
                     /gene="metG"
                     /locus_tag="JNC24_003859"
     CDS             complement(878..2911)
                     /gene="metG"
                     /locus_tag="JNC24_003859"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HAY9160491.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRSDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPQLYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKAAAAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            3043..4152
                     /gene="apbC"
                     /locus_tag="JNC24_003860"
     CDS             3043..4152
                     /gene="apbC"
                     /locus_tag="JNC24_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HAY9160492.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLNALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            4397..4678
                     /locus_tag="JNC24_003861"
     CDS             4397..4678
                     /locus_tag="JNC24_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HAY9160493.1"
                     /translation="MNKYWLSGIIFLAYGLASPAFSSETATLAINGRISPPTCSMAMV
                     NGQPQPHCGQLTYNVDTRHLFSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
BASE COUNT         1144 a         1177 c         1182 g         1231 t
ORIGIN      
        1 atatcaatct gttgaaggtg tattattaca tcagtagcat attccagacc gccttcctgg
       61 acgatctgat ccataacatc ctcacgcact ttatatcttg tgcatttcat ttcaagtgcc
      121 gtgagtacag ccattactgg tgatggtaac tgtgttgttt tcaggccgtc gatctgactg
      181 atcgcctggg cgacggtgtt ttgccattct aaagaacagg cgctgaagtc aaatgtggct
      241 gttttattat cccgttgcga cacactacag gctaagcgac gtaattgggt caatttgtct
      301 gcttgctgaa caggaactcc gggaaagcgg cgatgactta acgtggtgtg tacaacttcg
      361 ggcggaaaaa cgacagcaat atctttcgcc agccacggta aaacagcatc tgtatttttg
      421 tcaggcgcac gggtttctgc tacgaaagag ggttgttcct gaataatggt ggataagtcc
      481 gctaccttga gagagcccgt taccgtttgg gaaggttgca aagaattatc cttcgcttgc
      541 tccacatacc ccttcttcac cttctccgca atcagcttaa gctccgcttt tgccgctgcc
      601 gcagcatctg aaaaactttt tatctggctt tgccctttgg tgccaacttt tccccaactg
      661 atatgcaact cgtttccctg ttgctcaact gcccagaatt tatgtgattt ttcgtcctga
      721 tagataaagt gtctcattct gcatccctgt aattttatcc agttgaatgc agatgctacc
      781 agtatttatg cgggttagag agagacaaaa tcgcaagaaa acaactttag gaatttataa
      841 agaaaaggcg ctgtcgatgc agcgccttga agggggatta tttcacctga tggcctggtt
      901 tagcaccagc atccgggctt aacaggaaga tatctttccc gccaggacca gcagccatta
      961 ccatgccttc ggagatacca aagcgcattt tacgcggtgc caggttggcc accataatgg
     1021 tgtgacgccc gatcagtgcc tgcggatccg ggtaggcaga acgaataccg gagaagacat
     1081 tgcgtttttc accgccgaga tccagtgtca ggcgcagcag tttgtcagaa ccttcaacaa
     1141 actctgcgtt ttcgatcagc gccacgcgca ggtcaacttt tgcgaagtcg tcaaaggtga
     1201 tggtttcctg aatcggatca tctgccagtg ggccagtaac tggcgcggca gctgctttca
     1261 cttcttcttt agacgcttcc accagtgctt caacctgctt catatcgatg cggttataca
     1321 gcgctttgaa cggattcact ttgtggccca gcagcggttg ctggatacca tcccaggtca
     1381 gttctgtatt gaggaatgct tctgcgcgct cggtcagttt cggcagtacc ggcttcaggt
     1441 aagtcatcag cacgcggaac aggtttatgc ccattgagca aattgcctgc aggtcggcat
     1501 cgcggccttc ctgttttgcc accacccacg gagcctgctc gtcgacatag cggttagcca
     1561 gatcagccag cgccatgatt tcgcgcactg ctttaccaaa ttcgcggctt tcccacgctt
     1621 caccaatcac ttcagcggca tcagtgaagg ttttgtacaa ctgcgggtca gccagttcgc
     1681 ttgccagcac gccgtcaaaa cgcttgttga taaagcccgc attacgagag gccaggttaa
     1741 ccactttgtt cacgatatcg gcattcacac gctgaacgaa atcctccagg ttgagatcga
     1801 tatcatcaat gcgcgaagag agtttcgcag tgtagtagta acgcaggctg tcagcgtcaa
     1861 aatgattcag ccaggtgctg gctttaataa aggtgccgcg agacttggac atctttgcgc
     1921 cgttcaccgt cacatagcca tgaacaaaca ggttggtcgg cttgcggaag ttgctgcctt
     1981 ccagcatggc aggccagaac aggctgtgga agtaaacaat atctttaccg atgaagtggt
     2041 acagctcggc ggtggagtct ttcttccagt attcatcgaa gcttacgctg tcgctgcgct
     2101 tgtcgcacag attcttgaaa gaacccatgt agccaatcgg tgcgtccagc cagacgtaga
     2161 aatatttgcc cggcgcgttc ggaatttcaa aaccaaagta tggcgcgtcg cgggagatat
     2221 cccactgttg caggccggat tcaaaccact cctgcatttt atttgccacc tgctcctgca
     2281 acgcaccgct gcgggtccat gcctgcaaca tttcgctgaa agagggcaga tcaaagaaga
     2341 agtgttcaga atcacgcatt accggcgtag cgccagaaac caccgatttc ggctcgatca
     2401 attcagtcgg gctgtaggtc gcgccgcaga cttcgcagtt atcgccgtat tgatccgggg
     2461 atttacattt cgggcaggtg cctttcacaa aacggtccgg caggaacatg cctttttccg
     2521 gatcgtacag ctgagagatg gtgcggtttt taataaaacc gttctctttc aggcgagagt
     2581 agataagttc tgacaactgg cggttctctt cgctgtgcgt cgagtgatag ttgtcatagc
     2641 tgatgttaaa gcctgcgaaa tcagtctgat gctcctgact catttcgcca atcatctgct
     2701 ccggggtgat accaagctgc tgagctttca gcatgatcgg tgtaccgtgg gcatcgtcgg
     2761 cgcagatgaa gttgacctcg tggccgcgca ttcgctggta acggacccag acatcagcct
     2821 ggatgtgctc cagcatatgg ccgaggtgga ttgagccgtt agcgtacggc agtgcgcacg
     2881 tcaccagaat tttcttcgcg acttgagtca tagtaggcat tacttcttaa tagtgaaaag
     2941 gggttagata gtaccaaatg ggaaaatgtt aagtaagtac ttctctatat tcttcaggta
     3001 aactatcagc tgatggttta tctatacaaa aaaggagtcg ggatgaacga acaatcccag
     3061 gccaaatcac cagaagcctt gcgcgcaatg gtcgccggga cactggccaa ttttcagcac
     3121 ccaaccctga agcataacct caccacgctg aacgcgttgc accatgtcgc ctggatggac
     3181 gacacgttgc atgtggaact ggttatgcca ttcgtctggc atagcgcgtt tgaagagtta
     3241 aaagagcaat gtagcgccga actgctgcgt atcaccgggg cgaaagctat cgactggaaa
     3301 ctgtcgcaca atatcgccac cttaaaacgc gtgaagaatc agccgggtat taatggtgtg
     3361 aagaacatca ttgcggtcag ttccggtaaa ggcggcgtgg gtaaatcctc cacggcggta
     3421 aacctggcgc tggcactggc ggcggaaggg gcgaaagtcg gcattctgga cgccgatatc
     3481 tacggcccat caattcccac catgctgggc gcagaaaatc agcgtccaac ttcgccggac
     3541 ggtacccata tggcaccgat tatgtctcat ggcctggcaa ccaattcaat tggttatctg
     3601 gtcactgacg acaatgcgat ggtgtggcgt ggaccgatgg cgagcaaggc gttaatgcag
     3661 atgttgcagg aaaccttgtg gccggatctc gactatctgg tgcttgatat gccgccaggc
     3721 accggtgaca ttcaattgac gctggcgcaa aacattccag taactggtgc agtcgtggtc
     3781 accaccccgc aggacatcgc tttgatcgat gcgaaaaaag gcattgtgat gttcgagaaa
     3841 gtcgaggttc cggtgcttgg tattgtcgag aacatgagtg tgcatatttg cagtaactgt
     3901 ggtcaccatg aaccgatttt cggtaccggt ggtgcagaga aactggctga aaaataccac
     3961 acacagctgc tggggcagat gccgttacac atctccttac gtgaggatct cgataaaggc
     4021 acgccaacgg ttatcagccg tccggagagc gaatttaccg ctatctaccg ccagctggct
     4081 gaccgcgttg cagctcagct ctactggcag ggtgaagtca ttccaggcga gatttccttc
     4141 cgcgcggtct aacgcctatt aaccattctg gttattcttc atacaccgca gagatgttac
     4201 attgatgcgg tgtattagtt tttgccgcat taatgaacac ttatttaata ctgcgaatat
     4261 tctgcggacc ttcgtccgaa actctacaaa gtcgattgat catacaggca atgcttcatt
     4321 atcatccttc gtggctgtgt agtggatgta cagctatagt ttgttatgca ctcattctat
     4381 ccaggcataa ccttcaatga ataagtattg gctgtcagga attatttttc ttgcctatgg
     4441 attggcttct cccgcattct cttcagagac tgccacatta gcgattaatg gcaggatcag
     4501 tcccccaaca tgcagtatgg ctatggttaa tggtcaacct caaccgcatt gtggtcagtt
     4561 aacgtataat gttgacaccc gtcatctgtt ttcttcgccc gtcaaaggtg taacgacaga
     4621 agtggttgtt gcaggtagcg atagcaaacg tcgaatcgtg ttaaatcgct acgattaatt
     4681 tggcaacctg atttttattg atatcaacaa taccatttac ataaatctac atta
//
LOCUS       DADMKF010000223         4607 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.227, whole genome shotgun sequence.
ACCESSION   DADMKF010000223 DADMKF010000000
VERSION     DADMKF010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4607
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.227"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            <1..2162
                     /locus_tag="JNC24_003862"
     CDS             <1..2162
                     /locus_tag="JNC24_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HAY9160494.1"
                     /translation="GTIAWSILSSHNTSGNMDKLKIKFDSLASHDITFVGIVQTAKAS
                     GMERFPLPYVLTNCHNSLCAVGGTINGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMRE
                     MMAGGGKMILGSDSHTRYGALGTMAVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKP
                     APYVGPQDVALAIIGAVFKNGYVKNKVMEFVGPGVSALSTDFRNSVDVMTTETTCLSS
                     VWQTDEEVHNWLALHGRGQDYCQFNPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYE
                     IDTLNQNLTDILREIEIESERVAHGKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENV
                     IAAANALRGQSCGNDTFSLAVYPSSQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFG
                     AGDTPINNGLSIRHTTRNFPNREGSKPANGQMSAVALMDARSIAATAANGGYLTSASE
                     LDCWDNVPEYAFDVTPYKNRVYQGFVKGATQQPLIYGPNIKDWPELGALTDNIVLKVC
                     SKILDEVTTTDELIPSGETSSYRSNPIGLAEFTLSRRDPGYVGRSKATAELENQRLAG
                     NVSELTEVFARIKQIAGQEHIDPLQTEIGSMVYAVKPGDGSAREQAASCQRVIGGLAN
                     IAEEYATKRYRSNVINWGMLPLQMAEVPTFEVGDYIYIPGIKAALDNPGTTFKGYVIH
                     EDAPVTEITLYMESLTAEEREIIKAGSLINFNKNRQM"
     gene            complement(2396..3679)
                     /locus_tag="JNC24_003863"
     CDS             complement(2396..3679)
                     /locus_tag="JNC24_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HAY9160495.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEAQNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNMYYGFLAVNSRFN
                     AFGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(3814..4506)
                     /locus_tag="JNC24_003864"
     CDS             complement(3814..4506)
                     /locus_tag="JNC24_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAY9160496.1"
                     /translation="MLNGYIDEGKAASAKLIRSTLSDAFREAIAEGHITTKPVAATRA
                     AKSEVRRSRLTADEYLKIYQTAESSPCWLRLAMELAVVTGQRVGDLCEMKWSDIVDGY
                     LYVEQSKTGVKIAIPTTLHVDALGISMKETLDKCKEILGGETIIASTRREPLSSGTVS
                     RYFMRARKASGLSFEGDPPTFHELRSLSARLYEKQISDKFAQHLLGHKSDTMASQYRD
                     DRGREWDKIEIK"
BASE COUNT         1093 a         1141 c         1194 g         1179 t
ORIGIN      
        1 aaggcactat tgcctggtct attctctctt ctcataatac gtccggaaat atggataaac
       61 ttaaaattaa gtttgattca ttagcctctc acgatattac ctttgttggt attgtacaga
      121 ccgctaaagc gtccggaatg gaacgtttcc cgctgccgta tgtgctgacc aactgccata
      181 actcactctg cgccgtcggc ggcaccatta acggtgatga ccatgttttt ggtttatcgg
      241 cagctcagcg ttatggcggt atttttgtgc ctccgcatat tgcggtcatc catcaatata
      301 tgcgtgagat gatggcaggc gggggcaaaa tgatcctcgg gtcagacagc cacacccgtt
      361 acggtgcatt agggacaatg gcagtcggtg agggtggcgg tgagttggta aaacagctgc
      421 ttaatgacac ctgggatatc gactatccgg gcgtggttgc ggtgcatctg accggaaaac
      481 ctgcgccgta tgtggggcca caggatgtgg cgctggctat cattggcgcg gtgttcaaaa
      541 acggttacgt caaaaacaaa gtcatggagt tcgttggacc gggcgttagc gcgctctcta
      601 ccgatttccg taacagcgtt gacgtgatga ccacggaaac cacctgttta agctccgtct
      661 ggcaaactga tgaagaagtg cataactggc tggcgctgca cggtcgcggc caggattact
      721 gccagtttaa ccctcaaccg atggcgtact acgatggctg catcagcgtt gatttaagcg
      781 ccatcaaacc aatgattgcg ctgccgttcc acccgagcaa cgtgtatgaa atcgacacac
      841 tgaaccagaa cctgaccgac attctgcgtg agattgaaat tgagtccgaa cgcgtggcgc
      901 acggtaaagc caaactctcg ctgctggata aagtggaaaa tggtcgcctg aaagtgcagc
      961 aggggattat cgcgggctgt tctggcggta actacgaaaa cgtcatcgcg gcggcgaatg
     1021 cactgcgcgg tcaatcctgt ggcaatgaca ccttctcgct ggcagtttac ccgtcatcac
     1081 agccggtgtt tatggatctc gccaaaaaag gtgtggtagc agatttgatt ggcgcaggcg
     1141 caatcatcag aaccgcgttc tgcggcccat gctttggcgc gggcgatacg ccaatcaaca
     1201 acggtttgag tattcgccac accacgcgta acttcccgaa ccgcgaaggc tctaagccag
     1261 ctaatgggca gatgtcagcg gtggcgttga tggacgctcg ttctatcgct gcgactgcgg
     1321 caaacggtgg ctatttaacc tctgccagcg aacttgattg ctgggacaac gtgccggagt
     1381 acgccttcga tgtaacgccg tataaaaacc gtgtttatca gggctttgtg aaaggggcaa
     1441 ctcagcaacc gctgatttac gggccgaaca ttaaagactg gccggaattg ggtgcgctga
     1501 ctgacaatat cgtcctgaaa gtgtgctcga agatcctcga cgaagtgacc accaccgacg
     1561 aactgattcc ttccggtgaa acctcttctt atcgttcaaa tccgattggt ctggcggagt
     1621 ttaccctgtc tcgccgtgat cccggttatg ttggcagaag taaagcgact gctgagctgg
     1681 aaaatcagcg tctggcgggg aatgtcagcg agctgacaga ggtgtttgcg cgcattaagc
     1741 agattgctgg tcaggagcat attgatccgc tgcaaactga aattggcagc atggtctatg
     1801 cggtgaaacc aggcgatggt tctgcgcgtg aacaggcggc gagctgccag cgtgtgattg
     1861 gcggtctggc gaatattgcc gaggagtacg cgactaaacg ctaccgttct aacgtcatca
     1921 actgggggat gttacctctg cagatggcgg aagtgccaac ctttgaagtg ggggattaca
     1981 tttacatccc tggcattaaa gcggcgctgg ataatccggg tacgacgttt aaaggttatg
     2041 tgatccatga agatgcgccg gtaacggaaa ttacgctcta tatggaaagt ctgactgctg
     2101 aagagcgcga gattatcaag gcgggtagtt tgattaactt caataaaaac cgtcagatgt
     2161 aaaaagcgcc atgtgaatgt aggtcgcatt cggcacttat tgtcggatgc gatgcttgcg
     2221 catcttatcc gacctacgaa tcgcatcgaa tctgtaggcc agataaggca tttacgcagc
     2281 atccggcact tattgtcgga tgcgatgctt gcgcatctta tccgacctac aaatcgcatc
     2341 gaaccgtagg ccggataagg cgtttacgcc gcatccggca aatagttaat tgctcttact
     2401 tcttcgcctc tgcaaccact ttactaccca cgccgcggtt attgtattcc cacatgcggt
     2461 tgtagttagt gtcattcaga ttgcgctgta tttcgtcgtt atcatctacg ctgccggtat
     2521 tacccgcaaa cggacgatta gagatcaccg catcggccca cggtttagcc gtgttaaaac
     2581 cttcgttgat ggcgctatca cggatcacca cctgaccgtt ggtattggca tcaacatcca
     2641 gcgagcggcc cagttgcgcc acaccatcac cgaaagcatt gaaacggctg tttacggcga
     2701 ggaaaccgta gtacatgttg gacagcgtag ccggtgcaaa cacatacgct tcttgctgag
     2761 tacgtgagtt caccacgcgg aattcggtgt tatcgaacac cactgcgccg cgaccagaaa
     2821 cgatatccac atccccttca atgtagctgt tggtcaccag cgtacgcggc tgacgattcg
     2881 tttccagacg gttctgcaca ccgctgttgg tgacaaagaa ggtgttctga cgaccgagaa
     2941 tgttaacgtt gttaatctgt acctggtcac catcagtacg cagtgccacc gccggatggt
     3001 tacctgcatc tacgctatcg cccagcgtgt tttcgatggt cagattttgc agttgcaggc
     3061 cattgttttg tgaccagaag accgcagagc agagaacacc gatactgtcg ctgcgtttgc
     3121 tctggcagct atcgtacata taccacgctg gtttacctgg catatatttg ccgcgcgggt
     3181 tgacgtcgtg acgccagtcg gcagggctca tgccaccatc aagggaaagc ccaatcttca
     3241 catcaatcgg tttttcacct gtaccgtaca gagtaattcc acccggagcg gcagggacat
     3301 ataccgttcc ctgatactca ccaggcatca cggcaatata ctggcgcttg ttggtacgct
     3361 tgataattgc cgcatctacc gccgcctgaa tcgtggtatg cgttacacct tgagtgcccg
     3421 ccgggccgac aacaaagtca ggttgcgcag gcagggtaat cggggaagga ttccacgctg
     3481 cagcacctgg tgtcagggat gcaaaatagt gttgagcatc gaaattctgc gcttcttttg
     3541 ccgacagaat cgggcgagaa gaggtaccag gcgcggtttg atcagaagga cgttgatcgg
     3601 gcggggttga gctacaggcg gtcagcgtca cgccaaaagc caatgccagc gccagacggg
     3661 aaactgaaaa tgtgttcaca ggttgctccg ggctatgaaa tagaaaaatg aatccgttga
     3721 agcctgcttt tttatactaa gttggcatta taaaaaagca tcgcttatca atttgttgca
     3781 acgaacaggt cattatcagt caaaataaaa tcattatttg atttcaattt tgtcccactc
     3841 cctgcctctg tcatcacgat actgtgatgc catggtgtcc gacttgtgcc cgagaagatg
     3901 ttgagcaaac ttatcgctta tctgcttctc atagagtctt gcagacaaac tgcgcaactc
     3961 gtgaaaggta ggtggatccc cttcgaagga aagacctgat gcttttcgtg cgcgcataaa
     4021 ataccttgat actgtgccgg atgaaagcgg ttcacgacga gtagatgcaa ttatggtttc
     4081 tccgccaaga atctctttgc atttatcaag tgtttccttc attgatatcc cgagagcatc
     4141 aacatgcaat gttgtaggga tggcaatttt tacgcctgtt ttgctttgct cgacataaag
     4201 atatccatct acgatatcag accacttcat ttcgcataaa tcaccaactc gttgcccggt
     4261 aacaacagcc agttccattg caagtctgag ccaacatggt gatgattctg ctgtttgata
     4321 aattttcagg tattcgtcag ccgtaagtct tgatctcctt acctctgatt ttgctgcgcg
     4381 agtggcagcg accggttttg ttgttatatg gccttcagct attgcctctc ggaatgcatc
     4441 gctcagtgtt gatctgatta acttggctga tgccgccttg ccctcgtcta tgtatccatt
     4501 gagcattgcc gcaatttctt ttgtggtgat gtcttcaagt ggagcatcag gcagccccct
     4561 ccttattgct ttaattttgc tcatgtaatt tatgagtgtc ttctgct
//
LOCUS       DADMKF010000224         4573 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.228, whole genome shotgun sequence.
ACCESSION   DADMKF010000224 DADMKF010000000
VERSION     DADMKF010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4573
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.228"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(<1..211)
                     /locus_tag="JNC24_003865"
     CDS             complement(<1..211)
                     /locus_tag="JNC24_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse2/CasB"
                     /protein_id="HAY9160497.1"
                     /translation="MSIVKEEHKATLRKWHEELQEKRGERASLRRSTTVNDVCLTDGF
                     RLFLKNRQIKWQDEPEWRITALALIA"
     gene            complement(208..1769)
                     /gene="casA"
                     /locus_tag="JNC24_003866"
                     /pseudo
     CDS             complement(208..1769)
                     /gene="casA"
                     /locus_tag="JNC24_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311641.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse1/CasA"
     gene            complement(1867..4566)
                     /locus_tag="JNC24_003867"
     CDS             complement(1867..4566)
                     /locus_tag="JNC24_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HAY9160498.1"
                     /translation="MRKYPLSLLKDKNIVTFFDFWGKTRRGEKDGGDDYHLLCWHSLD
                     VAAMGYLMVKRNCFGLADYFRQLGISDKEQAAQFFAWLLCWHDIGKFARSFQQLYLPP
                     ELKIQEGARKNYEKISHSTLGYWLWNHYLSECQELLPSSSLSPRKLRRVIEMWMPVTT
                     GHHGWPPDRMDELDNFLPEDKAAARDFLLEIKPLFPLIEIPAFWDDDEGIELIKHLSW
                     YISATVVLADWTGSSTRFFPRVAHPMDIKGYWQKTLVQAQNALTVFPLKAKVAPFNGI
                     NTLFPFIENPTPLQQKVLDLDISQQGPQLFILEDVTGAGKTEAALILAHRLIAAGKAQ
                     GLFFGLPTMATANAMYDRLVKTWLAFYSPESRPSLVLAHSARTLMDRFNESLWSGDLV
                     GSEEPDEQTFSQGCAAWFADSNKKALLAEIGVGTLDQAMMAVMPFKHNNLRLLGLSNK
                     ILLADEIHACDAYMSCILEGLIERQARGGNSVILLSATLSQQQRDKLVAAFARGTEGL
                     QEAPFLEKDDYPWLTHVTKSDVNSHRVATRKDVERSVSVGWLHSEQECIARIESAVSQ
                     GKCIAWIRNSVDDAIKVHRQLLARGVIPASSLSLFHSRFAFSDRQRIETETLARFGKE
                     DSSQRAGKVLICTQVLEQSVDCDLDEMISDLAPVDLLIQRAGRLQRHIRDINGQLKRD
                     GKDERSPPELLILAPVWDDSPGDEWFGSAMRNSAFVYPDHGRIWLTQRVLREQGAIQM
                     PHAARLLIESVYGEDVVMPEGFARSEQEQVGKYYCDRAMAKKFVLNFRPGYAANINDY
                     LPEKLSTRLAEESVSLWLATCIDGVVKPYATGAHAWEMSVVRVRRSWWKKHRDEFSLL
                     EGEAFRLWCIEQRQDPEMANVILVNDDESCGYSATEGLIGKVG"
BASE COUNT         1132 a         1250 c         1050 g         1141 t
ORIGIN      
        1 cggctatcag cgccagtgcg gtgatacgcc actcaggttc atcctgccac tttatttgtc
       61 tgttcttcag aaacaggcga aatccatccg tcagacaaac atcattaacc gtcgtactac
      121 gccgcagact ggcacgttcg ccgcgtttct cctgcaattc ctcgtgccat ttgcgcagcg
      181 tggctttgtg ctcctctttt acaatactca ttcagcagcc tcctgctttt tctccctggc
      241 ggctttagca ctttgcttct ccgccgatgt tgtaaaatat ttcttgcgcg cggtcatgat
      301 gcgttccaaa tcaacgggct cataaggatt ggtgaataca cgctcgtcaa aatcctgacg
      361 cgcgaataac caaatttcct tattccattt gctgagtaat tcatccgcat cctgaccttc
      421 ttcaatttgg cgcactaacc tcaggaagcg atgctgagtt ttgttccaga agtcgatatc
      481 cacaaaacta aaatcacccc ttgcaccttt tggatcggag aaccatgctt ctttcaatgc
      541 actccgtaac agacttagaa tccgtgaagc cgtttgcgca gccagccgca gtttcggtat
      601 ctggccttct ttttattgag cagcagcggg aaatgatgtt cgtaccaaca gcgcgctttc
      661 atgttgtcga aatcatagcc aaatccccac aggcccactt ttgcctgctt gagactgctg
      721 gcattaaaga gtttcaccac cagcgcggga agttccgtat tgttttctga cttacccgtt
      781 tcgataaggc ctaaccagtc gcgccagatt aaaccgcccg gttgtggttt gaccgagtaa
      841 aactcgccgc cctctttaag tagtacacgg taaggcgtta agggatgctg ccacatggca
      901 tagttagcgc cgtaattttt ggtcgtcatc aggctcagaa gcgcgtcgct ctgctcaccg
      961 caaatatcgc agttgccgac tgtggtggta ttaaaatcaa tacgaatacg gcgaggcatt
     1021 ccccagtacg cctggagttt attgacctgc tcatcggtta ccaccgcacc ggccagttcg
     1081 ctgatgcgcg tcgggccaag ccaggggaaa accagatcgt caaatttttt gggtagcggt
     1141 aaatcggctt catcctgcgg catgacattg atccacagtt tgcgccacaa gggagtttgt
     1201 tgattaccct gatattcctg taattcaatc agagtggtca tcggcccacc gccgcgtaaa
     1261 ccggtgcggt agcctttgcc acctgacggc gcattcaact gtagggagaa cagagctaac
     1321 gcagagcaat gtgggcacag gtattccgtt acaccacgct tgataaagtg gtctttatta
     1381 aacttcgttg tttgagcgcc gggaatctca ggcagtagcg aagcgaccgg aactttatct
     1441 ccggtgagcg cctcgaaatc ctgtataaat gaaggggaat ctggcccaaa ctggaaagca
     1501 tgttctaatg acagcaatgc ttcccgtagc ttttcagctt ccagtccgtc ttcccagata
     1561 tcatcccaac gacgatgatc ttttggcgcg aagctggttt gtagtagccc cagcaaaaac
     1621 tgccataccg caccctggag atctgcccgc ggcgcagaaa tatcgacaac attttcatcc
     1681 gccagatcga ctggcgccag tttgcctgtc gttccgtctt taaaacgaac gggcaaccac
     1741 ggggttgtca gaagtgaaaa cgagttcata cgcatcctct cgtaaaaaag accatcccag
     1801 gtcggttgtc catctttcca tccttttcgg tgtatcaact attcagagag tcctgtcgca
     1861 gacatctcaa ccaaccttgc caatcaatcc ttccgtagcc gaatacccac aactttcgtc
     1921 atcgttgact aaaataacgt ttgccatctc cggatcttgt cgctgttcaa tgcaccacag
     1981 cctgaacgcc tctccctcca gtaaagaaaa ctcatcccga tgttttttcc accagcttcg
     2041 gcgcactctg acaacgctca tttcccatgc gtgagcaccg gtggcataag gcttcaccac
     2101 accgtcaata caggtagcca gccacaggga aacagactcc tcagccagac gtgtcgacag
     2161 tttttctggg aggtaatcgt tgatattggc ggcatagccg ggcctgaagt tcagcacaaa
     2221 ctttttagcc attgcgcgat cacagtaata tttgcccact tgctcctgct cgctgcgggc
     2281 aaatccttcc ggcattacca cgtcctcacc gtagacggat tcaataagaa gccgggctgc
     2341 gtgtggcatc tgaatagcac cttgctcacg cagcacacgc tgcgtcagcc agattcgccc
     2401 atgatcggga tagacaaatg cactgttacg catggcactg ccaaaccatt cgtcgccagg
     2461 agagtcgtcc cagacgggag ccagaatcag caattcaggg ggggaacgct cgtcctttcc
     2521 gtcacgcttt aactgaccat taatatcgcg gatatgccgc tgtaatcgtc ctgctcgctg
     2581 aatcagcaaa tcaacagggg ccaggtcgga gatcatttcg tccaggtcac aatcgacact
     2641 ctgctctaat acctgagtac aaataaggac ttttccggca cgctgtgagc tatcttcttt
     2701 accaaagcgt gccagtgtct ccgtttcaat tcgctggcga tcgctaaaag caaagcggct
     2761 atgaaaaagt gaaaggctgg aagcaggaat gacgccgcgg gcaagcagct gacgatgaac
     2821 cttaatagcg tcatcgacag aattccggat ccaggcgatg catttcccct gacttaccgc
     2881 cgattcgata cgcgcaatac actcttgttc actatgaagc caacccacgc tgacgctacg
     2941 ctcaacgtct ttgcgcgtcg ctacccggtg tgagttcaca tcggatttcg tgacatgcgt
     3001 cagccagggg taatcatcct tttccaggaa cggagcttct tgcaggccct ctgtaccccg
     3061 cgcaaaggca gcgacgagtt tgtcgcgctg ctgttgagat aacgtggcag aaagcaaaat
     3121 aacgctgttt ccgccacgcg cctgccgctc gatcaaccct tcaagaatgc acgacatgta
     3181 agcgtcacag gcgtggatct catcagccag caggattttg ttactcagcc ccagaagccg
     3241 cagattatta tgtttgaacg gcatcaccgc catcatcgcc tgatccagcg tgccgacgcc
     3301 aatttcagcc agcaacgcct tcttgttact gtcggcaaac caggccgcac atccctgact
     3361 gaacgtttgt tcatccggtt cttctgaccc gactaaatca ccagaccaga gtgattcatt
     3421 aaagcggtcc attaatgtgc gggcactatg tgccagcacc aggctggggc gggattctgg
     3481 cgaatagaaa gcaagccagg ttttgaccag ccgatcgtac atggcattgg ccgttgccat
     3541 tgttggcaga ccaaaaaaca gcccctgagc cttccccgca gctatcaacc tgtgcgccag
     3601 gataagcgcc gcttctgttt tacctgcgcc agtcacatct tccagaataa ataactgcgg
     3661 tccttgctgg ctgatatcca gatccagcac cttttgctgt aacggtgtcg gattctcaat
     3721 aaaaggaaac agcgtattaa ttccgttgaa aggcgcgact tttgctttta gaggaaagac
     3781 ggttaaggcg ttttgagcct gaactaaagt tttctgccag taacctttaa tatccattgg
     3841 gtgtgcgacg cgtggaaaaa atcgcgttga cgaacccgtc cagtctgcga gcacgacggt
     3901 tgcagagata taccaggaaa ggtgttttat aagttcaatg ccctcatcat catcccagaa
     3961 tgcggggatc tctatgagcg gaaacagtgg tttgatttca aggagaaaat ctcgtgctgc
     4021 agctttgtct tcaggcagaa aattatccaa ttcatccata cgatcagggg gccaaccatg
     4081 atgccctgtc gtcacgggca tccacatttc tatcactcgc ctaagtttgc gcggagagag
     4141 cgaagatgaa ggaagtaact cctgacattc acttaaataa tgattccaca accagtagcc
     4201 cagcgttgaa tgagagattt tttcgtaatt ttttctggca ccttcctgaa tcttgagttc
     4261 agggggcagg taaagttgtt gaaaagagcg ggcaaatttt ccaatatcgt gccagcacag
     4321 caaccaggcg aaaaattgag ccgcctgttc cttgtcagaa atccctaatt gacgaaaata
     4381 atcagccagc ccgaagcaat ttcttttaac cattaaatag cccatcgcgg ctacatccag
     4441 cgaatgccag caaagaaggt gatagtcgtc gcccccgtct ttctcgccgc gtcgggtttt
     4501 tccccagaaa tcaaaaaaag tcacaatatt tttatccttc agtaaactta aaggatattt
     4561 acgcatatat taa
//
LOCUS       DADMKF010000225         4547 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.229, whole genome shotgun sequence.
ACCESSION   DADMKF010000225 DADMKF010000000
VERSION     DADMKF010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4547
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.229"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            823..1712
                     /locus_tag="JNC24_003868"
                     /pseudo
     CDS             823..1712
                     /locus_tag="JNC24_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000049841.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            1712..2002
                     /locus_tag="JNC24_003869"
     CDS             1712..2002
                     /locus_tag="JNC24_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000666098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9160499.1"
                     /translation="MKEYHGEKRYKDYLLKRYSISREGHLMKDTHGEVYRIRPKKEGR
                     GYFFYDGVTDLKIDALRFAVLYHFDIWNSIHQLRLKDGDPSNLKATNIITKR"
     gene            2323..2613
                     /locus_tag="JNC24_003870"
     CDS             2323..2613
                     /locus_tag="JNC24_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000149936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9160500.1"
                     /translation="MRHTVTRLLPEAYSRLAAIKDKYPAMSHTALVGEILKEHIALTP
                     FDAIKGVAEGSKAIRITTGQLELLKAQSAVAGCSMIQVLSAKVHQLADKLGV"
     gene            2613..3032
                     /locus_tag="JNC24_003871"
     CDS             2613..3032
                     /locus_tag="JNC24_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000882667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9160501.1"
                     /translation="MLCTFDYHAFYRHRGHLVRENNGTWKDLTRNAEPFTHQQLKSRF
                     SRWKNAPADTGDFEIIHTFDYASEDDLHDAIKLIEDFNTSEDVDGDKYKSRGNRYNRD
                     DITQAINAGQSQRYITEKYGISRRQYFYIKPLINKDN"
     gene            3200..3523
                     /locus_tag="JNC24_003872"
     CDS             3200..3523
                     /locus_tag="JNC24_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9160502.1"
                     /translation="MNNYPMQIMVDNDTAMMVQSFIDAGIAINFDKLLKLMADNVENI
                     SDFIQSVEFNEPRMMLPITDSNMKRLVIEQTNIYSISPEQFLKGAVTILYSDNILVTD
                     SVRVH"
     gene            3953..>4547
                     /locus_tag="JNC24_003873"
     CDS             3953..>4547
                     /locus_tag="JNC24_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9160503.1"
                     /translation="MIRNEHEMLIEHRPSALDIHELTLRESFDRAWRESHEVNKGPYI
                     PPEPLEIPRVEIDFSMNERCELDLKLKLQRRYFQAIKDSQEAMRSVYEKIQSKLEDEI
                     DEMVISMELQTNSYAYFSRLDRDGWGYDPVEIGKVIADLPEGQRVVRTERAVRAPHRL
                     SLIVADKSQAELEEIAKEKLMKARENEINLLKSRLAKL"
BASE COUNT         1499 a          836 c          931 g         1281 t
ORIGIN      
        1 ccgtacagcg atattccgtc gttcctctta aaagcgtctg aagcggattt agtagctttc
       61 ctgataccgc atagagccaa caccagctac tggcttaggc acatttacag caataaccac
      121 tgtcatgaaa tccgtattct tcatcgtgca gttaaatacc ttccaccagc agggcataac
      181 aggcttacta ttaggtcacc gtttccaagt gcagtcgttg tttttaaaaa agaaccacgt
      241 aagcatgaaa ttacacagat ggtttgctgt gctgatacat tgctaccgtt aaccatcatt
      301 aaccgtggtg gactaagagg aagacccacc atttacccac ctgagacgct cgacagcttc
      361 ataaaactat acaggcaagg aaaacccatt aagtacattg cagatgctct cagaatgcct
      421 ctgagcacgt catacaggat tgctcaaagg ttgagctaaa attctcataa atatggtcat
      481 ttacaagaca ggtgattcag atgttaagaa gtatccctgt gaacaataaa tgaccaatca
      541 gtaatcattc tgtgaaataa ttgttgcaat ttataatcgc atctgttata gtttctatat
      601 gggctgataa aaaagttcat gtcttgaagt ggtaacactt ggggaggtgt cagatggttg
      661 gcgtatatta acctctcatt cagggtatac ctcagcgttt taatcatcgt actgtttcag
      721 gcatggactt ctttatcagt ccatcaatcc cccagatatt ttaatcacac aggctaatcg
      781 gttttatccg gttggccttt tcacgtatga ggtaatttat gaatgtctaa atataaccca
      841 aatcagtttt ataaatttga tgtgcgaatc tctcaacttt cagaagaaat taacgcagca
      901 gcaagattga tttatatggt catggtccat agctgtgact tctatgaaga gaaatacaaa
      961 tcatattcac caagtattga ccatctcgct tatatggctg gtgtatcaaa gagaacagca
     1021 atcagagcca ttgagacact tgagaaacac ggtttaattc aggtgcaaaa gaatcagggg
     1081 cgtcaaagca cttatcgtgt attcacactg gatgataagc cggaattact tgtaagacct
     1141 acatcacagg aagttgcagc ctcagtaaga caaaatgaca ccactaccag tgacaaaatg
     1201 tcatgggggt agtgacaaaa tgtcacccgt gttagagttt agataagtta ttagataaaa
     1261 cattagataa taatattaca gataagaata aagagactgg acttaatgat gatattaaag
     1321 aagatgactt tctttctttg gatgaattaa atgattttct ctctatgaat gaatctgacc
     1381 ttgaagtaag ctctaaaaag acttctaaac aagacagaga aatcactgat gtgagtgttg
     1441 atagtgacca taaaaacaca cagtgccccg ccactgatga tgagttggac cccttcgatg
     1501 atgaagagga tgaacgtgaa aaagccatta agagggcaga gaagaaaaag agattcgagc
     1561 ttgcaagaca aaagaacaaa gtatcgggtt tagacccatt cgatgacagt gaagatgatt
     1621 attatctttc accgaaaaag aaacaaactc aggatgacaa gaagcaaaaa attaaaccga
     1681 agatgtattt tgccggtgga ggaattaagt aatgaaagaa tatcacggtg aaaagagata
     1741 caaagattac ctgttaaagc gttacagcat ttctcgtgaa ggtcatctta tgaaggatac
     1801 tcacggggaa gtttaccgga ttagaccaaa gaaagaaggg aggggttatt tcttttacga
     1861 tggtgtgaca gatttaaaga ttgacgcttt gaggtttgca gtgctgtatc attttgatat
     1921 ctggaattca atccaccagc tacgattaaa agacggtgac cccagcaact taaaggccac
     1981 caatattatc acaaaacgtt agtttgtttt accatcatca atcgtaattg tgatttcata
     2041 tttgaaaagc ttaatgctga aattgaatcg tgtacttctg gcgataatca tttttaaaag
     2101 ctgatacaca agaagcacca gaaacacaaa gcaacctaag cttgcgaatc tgtgagattc
     2161 agcaattgtt tcaagaaagc ccattaattc cccttattta tcaaagggaa tgacctgtat
     2221 cagcccccta caaccatatt aacatgtaaa tgagttgttg acaatatgtt ataaatatgt
     2281 taaagttagt tataagtcaa agattttaag aaggaaaatt taatgagaca cacagttaca
     2341 cgccttttgc cagaggcata ttccagactg gcagcaatca aagacaaata cccagccatg
     2401 agccacacag cacttgttgg tgagattttg aaggaacaca tagcccttac accgtttgac
     2461 gctatcaagg gcgttgcaga gggttctaag gccatcagaa tcacaacggg ccaactggaa
     2521 ctactcaaag cacagtccgc agtggcaggt tgctcaatga ttcaggtgtt gagtgccaag
     2581 gttcatcaac tggcggataa gctgggggtt taatgctatg cacatttgat tatcatgcgt
     2641 tttatcgcca tagaggtcac cttgtccgtg agaataacgg aacatggaaa gatttaactc
     2701 gtaatgctga acccttcaca caccagcaat taaaatcccg cttctccaga tggaagaatg
     2761 caccagcaga tactggtgat tttgagatta tccatacctt cgattatgcc tcagaagatg
     2821 accttcacga tgcaattaag ctgattgaag acttcaatac ctcagaagat gtggatggtg
     2881 acaaatataa atcccgtggt aaccgctata accgtgatga tatcactcag gccataaatg
     2941 ctggtcaatc tcagcgatac attacagaaa aatatggtat ttcacggaga cagtatttct
     3001 acattaagcc attgattaat aaagacaatt aaaaataacc atctaagaaa cggtagaatg
     3061 cactttacag gcagagttgc accttaattt gataaattac cttaccagcc agtgcaactc
     3121 cctctgtaac ccgcatggtt actggcctca gaatggctat tttaagaggt ctgaggtgtg
     3181 tttcatagga gatttaaaaa tgaataatta cccaatgcaa attatggttg ataacgacac
     3241 tgctatgatg gtgcaaagct tcattgatgc aggtatcgca attaattttg ataaattact
     3301 aaagctgatg gctgataatg tagaaaacat ctctgacttt attcagtcgg tggagtttaa
     3361 cgagcctcga atgatgctac caattacaga cagcaacatg aaaagattgg ttatcgagca
     3421 aacgaacata tacagtattt ccccagagca atttttgaaa ggtgctgtga caatcctgta
     3481 cagtgataat attctggtga cggattctgt gagggttcac taatgggttt aataattgct
     3541 tggacgttgc ttggcttatt tgttggttgt attattaaca atgcggtaaa taagaattga
     3601 tgttatcaac ctgttaaaaa gctgttgaca aataacctta agcatggcat aattttaatt
     3661 atgggagagc aattctctct tatttttctt tcaataatcc gatggatgga tattctgagt
     3721 tggaaaggtg caatgctttt cgtcatggcc tttcaggtta gtctgagagg ctatctcaaa
     3781 aagtactctt gttcattcat tattgaatcc tttgcccttc acagaaatgt ttagggcttt
     3841 ttcagttttt aaaagcacac ttggcaggtt cttgttcagt cagactcttc acctctgatt
     3901 gctgagtgtg cttctataaa ttgaaataac aaataaacta aggggtgaac gaatgattag
     3961 aaatgaacat gaaatgctta tcgaacacag accaagtgca cttgatattc acgaattaac
     4021 tttgcgtgaa tcttttgata gagcttggcg ggaatcacac gaagtaaata aaggtccata
     4081 tattccacca gaaccactcg agattccaag agttgaaatt gatttttcta tgaacgaacg
     4141 gtgtgaactt gatttgaagt tgaagctaca acgccgatat ttccaagcta ttaaagactc
     4201 tcaagaagca atgagaagtg tttacgagaa aattcagtcc aagcttgaag acgaaattga
     4261 cgaaatggtt atttcgatgg agcttcaaac caattcttat gcttatttct ctcgactaga
     4321 ccgtgacggt tgggggtatg accctgtaga gattggtaaa gtgattgcag atttaccaga
     4381 aggtcaaaga gttgttcgta cagaaagagc tgtcagagca ccacatcgac tgtctctaat
     4441 tgttgctgat aaatcacaag ctgagcttga agaaattgcc aaagagaaac tgatgaaagc
     4501 aagagaaaat gagattaatt tgctgaaatc aagactggct aagctga
//
LOCUS       DADMKF010000226         4534 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.230, whole genome shotgun sequence.
ACCESSION   DADMKF010000226 DADMKF010000000
VERSION     DADMKF010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4534
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.230"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(146..2083)
                     /locus_tag="JNC24_003874"
     CDS             complement(146..2083)
                     /locus_tag="JNC24_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HAY9160504.1"
                     /translation="MNIAASSELVSRLSTHRRVVALGDTDFTDVAAVVITAADSRSGI
                     LALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPPFYD
                     TLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGDLLI
                     HEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKSNHH
                     GALIQAGATSVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKAELPRPFRLAI
                     IQLGTYDGTVYNARQVIDTVGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNENDP
                     GIFVTQSVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYPLFA
                     ALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDYPTS
                     VLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGINAETGEYSDFGVPATI
                     LAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSPLAEVLP
                     SVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAHSAD
                     MTFRVKPESLILTIFFLGLIP"
     gene            2494..3201
                     /locus_tag="JNC24_003875"
     CDS             2494..3201
                     /locus_tag="JNC24_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HAY9160505.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLFGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            3307..3382
                     /locus_tag="JNC24_003876"
     tRNA            3307..3382
                     /locus_tag="JNC24_003876"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3340..3342,aa:Phe,seq:gaa)
     gene            3580..>4534
                     /locus_tag="JNC24_003877"
     CDS             3580..>4534
                     /locus_tag="JNC24_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HAY9160506.1"
                     /translation="MALTDAKIRAAKPTDKAYKLTDGAGMFLLVHPNGSRYWRLRYRI
                     LGKEKTLALGVYPEVSLSEARTKRDEARKLISEGVDPCEQKRAKKVVPDLQLSFEHIA
                     RRWHASNKQWAQSHSNKVLKSLETHVFPFIGNRDITTLNTPDLLIPVRAAEAKQIYEI
                     ASRLQQRISAVMRYAVQSGIIRYNPALDMAGALTTVKRQHRPALDLSRLPELLSRIGS
                     YKGQPVTQLAVMLNLLVFIRSSELRYARWSEIDIDNAMWTIPAEREPLPGVKFSHRGS
                     KMRTPHLVPLSKQAVAILTELQTWAGENGLIFTGAHDPRKPI"
BASE COUNT         1182 a         1195 c         1091 g         1066 t
ORIGIN      
        1 acccacgtta gcgcttggcg acggcggaac aaagctgatt cgcgacgtaa gaaagtggca
       61 ggctcatatg acggacctct gtaatgcggt atccaacccg cgaatatcag cgtgattcac
      121 cgtcagattt actgctacgt cgccctcagg gaattaaacc taaaaagaaa atcgtcaaaa
      181 ttagagattc cggtttgaca cggaaagtca tatcagcgct atgcgcatcc tgggggttca
      241 tcaccactga cgggaaactc tgctggcgga acatcgcttt ttgtaggtct ttgacgtcga
      301 aactgacata cagatcgtgc atctcctgac acaactggcg cagggtgtag tcgcgatagc
      361 gcaccggata tttgttataa acgctcggca acacctcagc cagcggcgag tcatcctcaa
      421 tatgctgttc aaattgcgcc agcatcgcca ccagttgtgc cagcttctcg tggctttccg
      481 ccggagttaa taagaacaga atggagttga gatcgcactt ctccggcaca atgccgttct
      541 cacgcagata gtgcgccaga atcgtcgccg gaacgccaaa gtcgctatat tcgccggttt
      601 cggcattgat acctggtgta gtgagtaaca gcttgcacgg atcaacaaaa tactgatccg
      661 cggcatatcc ttcaaagccg tgccacttcg cccccggctc aaaactgaaa aaacggcggt
      721 cgctggctaa cacagatgtc ggataatcct gccacaattt gccatcaaca acgggcggga
      781 taaacgggcg gaacagctta cagcgcgcaa gaatagcctt gcgcgactca atacccaact
      841 caacgcactc agcccacagc cgacgcccac tctccccttc atgaattttg gcgttaacat
      901 ccagtgcagc aaacagcgga tagaaagggc tggtagaagc atggagcata aaggcgttat
      961 tcaaccgctt atgcgggcaa aaacgcgcct gtccgcggat atggttatct tttttatgga
     1021 tctgcgacgt ctgtgagaat cccgcctgct gtttgtgcac cgactgagtc acaaagatcc
     1081 ccggatcgtt ttcgttaagt tctaatagca gcggcgagct atccgccatc atcgggataa
     1141 actgttcgta accgacccac gcggaatcaa acagaatgta atcacacaga tgcccaacgg
     1201 tatcgatcac ctgacgggcg ttatagacag ttccgtcata ggttcccagc tgaataatcg
     1261 ccaggcgaaa cgggcgcggc agctcggctt tttctggcgc aacgtcgcga atttgctggc
     1321 gcagatactc ttcattaaaa cagtgcgcat caataccgcc aatgaaacca aacgggttgc
     1381 gtgaagcttc cagatagacc gacgtcgccc ccgcctgaat cagcgcgccg tgatgattcg
     1441 acttatggtt gttacggtcg aagagcacca gatcgccacg cgttaacagc gcattcgtca
     1501 ccactttatt cgctgccgat gtgccgttca gcacaaaata ggttttatcg gcatgaaaga
     1561 ctttggctgc gaatttctgc gcatccttcg ccgatccttc atgaataagc agatcgccca
     1621 attttacgtc agcgttacac atatcggcgc gaaagacgtt ctcaccaaag aaatcgtaaa
     1681 aatggcgtcc ggcaggatgc tttttaaaaa acgcaccatg ttgatgtcca gggcaagcaa
     1741 aggtgctgtt gcccatctca acgtactgcg tcagcgtgtc ataaaacggt ggcagcaaat
     1801 tctcttcata ctgacaggct gcggattcca gctccagcca ctgctgctcg ttgccgttga
     1861 ttaccgccgt aacgcccgca ggtaattcaa cagcatgttc ggaatacaaa aacaccggta
     1921 gatgaaaacc ggtgcgctta agcaacgcaa gaatgccact gcgactatcc gcagcggtaa
     1981 tgacgactgc cgcgacatcc gtaaaatcag tatctcccaa cgccaccacg cggcgatgag
     2041 tagaaagtcg ggataccagt tcactactgg cggcaatatt cattgatttc ataagcgcaa
     2101 aacccgtttc ggggaagtaa gaataccgga caaagtgtaa aaccctgccc catgagatat
     2161 gggtcaaact ggtcaccagc tccgacgacc gccagacatc agtaaaagca gaaacgctct
     2221 gattttactg ttgtcctgca gtgaacgtgc gttaacttca ccgcatgagc agtaacatag
     2281 aaagggaaac gtttcgcgcg aaacggcgat aagcgagaga atgtaaggag atggcgtgca
     2341 tcgggcaaac tccgtacaga gaggagaaaa ttcgcgcaat catggcacct ttcgctaagg
     2401 cgtgcaagcc aggactttgc gaccagacaa gccatcaaca aggcaaaaag ttcataataa
     2461 gaaaatcaaa caatacagct aacaggaact cttgtggtca tcggcccttt tatcaacgct
     2521 agtgcagtct tactgggtgg cgttctcggc gcactgctca gccaacgctt accggaacgt
     2581 atccgcgtct ccatgacatc tatttttggt ctggcatcgc tggggattgg tattttactg
     2641 gttgtgaaat gcgccaacct tccagcgatg gttttagcta cgctatttgg cgctctaatt
     2701 ggcgaaattt gtttgctgga gaaaggtgtc aatacagcag tcgccaaagc acaaaatctg
     2761 tttcgtcact cacgtaagaa gccagcgcat gaatctttta ttcagaatta tgtcgcgatt
     2821 attgtcctgt tttgcgccag cggcaccggg atcttcgggg cgatgaacga agggatgacc
     2881 ggcgatccga gtattttaat cgccaagtca tttcttgatt tctttacggc gatgatcttc
     2941 gcctgctcgc tgggtattgc ggtgtcggta attagtatcc cattactgat catccagcta
     3001 acgctagcgt gggctgccgc gctgatatta ccgctgacca caccgtcgat gatggcagac
     3061 ttcagcgccg taggcggttt attgctgctg gcaaccggat tacgcatctg tggcattaaa
     3121 atgttcccgg tggtcaacat gcttccagca ctccttctgg caatgccgct ttccgccgcc
     3181 tggaccgcct ggtttgcctg acaatgcgtg caatagcggc aaagtgatga tagattgtgc
     3241 agtctgcagt aaattgaaga aatttgattg acgagacgag gcgaatcagg tttaatgcgc
     3301 cccgttgccc ggatagctca gtcggtagag caggggattg aaaatccccg tgtccttggt
     3361 tcgattccga gtccgggcac caaattcata tcaacggacc tccacggagg tccgtttttc
     3421 gtttcagaac gccacgattt aagcgttctg cctacaaatc aattctaccg aactcaacca
     3481 gattcccccc acatcaaccc cattgtgtgg gtataattgc gggcataccc cagttcgaca
     3541 gaatttgtac cctctttaac gcattcagaa ggctgaacta tggcactgac tgacgcaaaa
     3601 atccgggctg caaagcccac tgacaaggct tataaactca ctgacggggc tggcatgttc
     3661 ctgctggtac atcctaatgg ctcccgttac tggcgtctcc gttatcgtat tctgggtaag
     3721 gagaagactc tggcacttgg tgtgtatcca gaagtttctc tctccgaagc tcgtacaaaa
     3781 cgggatgagg cccgaaaact gatttcggag ggggttgacc cttgcgaaca gaaaagagct
     3841 aaaaaagtag tccctgattt acagctctct tttgaacata ttgcgcgacg ctggcatgcc
     3901 agtaataaac aatgggcaca atcacacagc aataaagtac tcaaaagcct tgagactcac
     3961 gttttcccct ttatcggcaa ccgggatatc acaacactca ataccccgga tctgcttatc
     4021 cctgttcgtg ctgcagaagc taaacaaatt tatgaaatcg ccagtcgtct gcagcaaaga
     4081 atatctgccg taatgcgtta tgccgtacag tctggcatca tcagatataa tcctgctctg
     4141 gatatggctg gcgcattgac tacggtaaaa cgccagcatc gccccgctct tgatctttct
     4201 cgcctgcctg aacttttgtc gcgtattggc agttacaaag ggcaacctgt cacccagctt
     4261 gccgttatgc tgaatttact ggtttttatt cgttccagtg aactcagata cgcccgctgg
     4321 tctgaaattg atattgacaa tgccatgtgg actattccag ccgaacgcga acccctgcca
     4381 ggcgtaaaat tctcacaccg gggctccaag atgcgaacac cacatcttgt gccactcagc
     4441 aaacaggctg tagccatact gacagaacta cagacatggg ctggtgaaaa tggtctgatc
     4501 tttacgggag cacatgaccc gcgtaaacca atca
//
LOCUS       DADMKF010000227         4481 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.231, whole genome shotgun sequence.
ACCESSION   DADMKF010000227 DADMKF010000000
VERSION     DADMKF010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4481
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.231"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(7..1068)
                     /locus_tag="JNC24_003878"
     CDS             complement(7..1068)
                     /locus_tag="JNC24_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01620 family protein"
                     /protein_id="HAY9160507.1"
                     /translation="MTEPLKPRIDFDGPLEVDQNPKFRAQQTFDENQAQNFAPATLDE
                     APEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGVQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWQCLWCLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(1065..2462)
                     /locus_tag="JNC24_003879"
     CDS             complement(1065..2462)
                     /locus_tag="JNC24_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HAY9160508.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSVKWRMMCEGLDPLAPADENRLADIAAAWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDTWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTVDQHANMVSLLQQLIQDAWQNAAFEGISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            2617..3615
                     /locus_tag="JNC24_003880"
     CDS             2617..3615
                     /locus_tag="JNC24_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAY9160509.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLAAHNIAVRD
                     ELIVDGGYTHETALQAARELLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            complement(3726..>4481)
                     /gene="ompG"
                     /locus_tag="JNC24_003881"
     CDS             complement(3726..>4481)
                     /gene="ompG"
                     /locus_tag="JNC24_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
                     /protein_id="HAY9160510.1"
                     /translation="AEPSVYFNAANGPWRIALAYYQEGPVDYSAGKRGTWFDRPELEV
                     HYQFLENDDFSFGLTGGFRNYGYHYVDEPGKDTANMQRWKIAPDWDVKLTDDLRFNGW
                     LSMYKFANDLNTTGYADTRVETETGLQYTFNETVALRVNYYLERGFNMDDSRNNGEFS
                     TQEIRAYLPLTLGNHSVTPYTRIGLDRWSNWDWQDDIEREGHDFNRVGLFYGYDFQNG
                     LSVSLEYAFEWQDHDEGDSDKFHYAGVGVNYSF"
BASE COUNT         1092 a         1228 c         1098 g         1063 t
ORIGIN      
        1 attgcattat ttttcgctgg gtgttttgcc tttttgcagt gtttctttca cctgaccgat
       61 aagctgacga cggaaatccc cgaggcgagg tttgtcatcg tcaatccacg gcagcgggcg
      121 gcaaagctcc atagctttaa tcccgagtcg tgccgtcaga agtcctgcac caatcccctg
      181 agctgcgcgg gtagacaaac gagcagcgag atcttgcgac atccagtcca tccccacttc
      241 gcgcaccagt tcgctggctc cggcaaaagc gatattcagc aataccagct taaacagacg
      301 caaacggctg taatacccca gttcaatgcc atacagcgtg gcgatgcgat taattaaacg
      361 cagattgcgc caggcgataa acgccatatc gaccaacgcc agcgggctga ccgcaatcat
      421 caacgttgat tccgccgccg aacggctgat ttcgcgccgc gcctgggcat ctaaaactgg
      481 ctggaccaaa tgcgcataca ggctgaccac ttcacggtcg ttttgcgttt catggattga
      541 ggcataccag cgttgcagcg ccggatgcga ctgatcaata cccgcctgct gcgccagttt
      601 ttcgcaaaat gcgcggcctt tgcccgtgcc gtggctatgc aataaatcac gcgcttcgtc
      661 gcgttcatgg gcgcgctgtc gcaagcacca taagcactgc cactctgtta ccacagaacc
      721 tacgccagcg ccgataatca atgccccagc ggcacatcca cccagcgcta cccagtcctg
      781 ggtttgccag gcattcattg tccactgtac accctgcccg acaacacttg cgccaaacag
      841 agccagcccg cccatcacca ttttgcgcca caggctgcgt ttcggacgta acgctgcatc
      901 cattaccgct tcaacttgcc cctcttcttc aggcgcttca tcgagcgtgg ccggggcaaa
      961 attttgcgcc tgattttcgt caaaggtctg ctgcgccctg aattttggat tctgatcgac
     1021 ctccagcgga ccgtcgaaat caatacgtgg ttttaacggt tcggtcatcg caatttatct
     1081 cctattaaaa attccagcgc ggcatcaaga cgaatatgcg gcagcggttt gtcgacatcc
     1141 atcacctgcg ggcgaaacgc ttcaaactgg aacccttgct tgtcccagaa cgcctgaccg
     1201 ggcaaacgcg caggaacttc accggggtaa acggtgagag gcgcaccatc gctgagccga
     1261 ttaccgcgca atgccggaat tttctcgccg ttgacatcaa taatgccgct ggtggtcgcc
     1321 tgcaccgagg ccagccccag gcaatccata ctaatccctt caaacgccgc attttgccag
     1381 gcatcctgaa tcagttgttg cagtaatgaa accatattgg cgtgctgatc gaccgttaca
     1441 tggtccgctt tcgttgcggc aaacagcagt ttgtcgataa ccggcgagaa taagcggcga
     1501 aagagcgtgc gctgcccgta gtggaaactt tgcatcagct gcgtcagtgc cagacgcata
     1561 tcattaaatg cctgtggccc actgttgaga ggttgcaggc aatccaccag cacaatctgg
     1621 cggtcaaagc gcagaaaatg attcttatag aaccccttca ccaccttctc gcagtaataa
     1681 ttaaaccgct cgcgcagcat tccggcattg gtgtgcttat cggcctgcgc cagtttggac
     1741 tcgccccagg tatcgacatc cggccacggg aagaattgca gcgcgggcgc acctgccata
     1801 tctcctggca agacaaagcg ccctggctga ataaagtgca gcccctgctg tttacagtgg
     1861 tggagataat cggtccacgc ggcggcaatg tctgccagcc ggttttcgtc ggcaggtgct
     1921 agcgggtcca gcccttcgca catcattcgc catttgaccg accattcgcc gcgctgacca
     1981 ttgagtaagc ccgtcatctg gcgtgaccag cttaaatagt cctgcgccag catcggcagg
     2041 tcgagcaacc attcgccggg ataatccaca atctccagat acagcgtgga ggtgtctttg
     2101 aagtggcgta gcagcgaatc gttcgattta aaacgtagcg ccaggcggat ttcactgacg
     2161 ccgcgcgtag gcgtcggcca ggcgggagga tcgccatata actgcgccag tccttcatca
     2221 taggtgaagc gcggaatgcc aaagtcacgc tgaggaatgc gttttacgcc cagcaggcgc
     2281 tcttcacgca ccgcgcttaa cagcggcaag cgtgctccgg cgtgaatatt gagcaactga
     2341 ttgaccatcg cagtgataaa cgctgttttg ccgctgcggc ttagtccggt tacggcgagg
     2401 cgcagatgtc tgtcgacacc ccgattcacc agcgcattaa gttcattttt aagtcgcttc
     2461 attgctgtcc tgtgctgctc tgaagaccag aaaaaaatca ttcgtaagga tatgaatgtg
     2521 ggggccaggc cattgattat caactgcccg tgttgtcatc gacgggcgaa ctccataaga
     2581 tgtgcagcat tcacgttcgg acggagttcc tgacctatgt cccctactat ttatgatatt
     2641 gccagggttg caggcgtatc aaaatccacc gtatcacgcg tgctgaataa gcaaactaat
     2701 atctccccgg aagcgcgcga aaaagtgtta cgggccattg aagaattaca gtatcagcca
     2761 aacaagctag cccgcgcgct gacctcttcg ggttttgatg ccattatggt gatttctacc
     2821 cgttcgacca aaactacggc gggtaatccg tttttctctg aagttttgca tgccatcacc
     2881 gccaaagctg aagaagaagg tttcgacgtg atattgcaga cgtcgcacaa cccggcagaa
     2941 gacttacaaa aatgtgaaag caaaattaag cagaaaatga ttaaaggcat tattatgcta
     3001 agttcgccag cggatgagtc attttttgcc caactcgata aatacgatat tccggtggtg
     3061 gtgattggca aagttgaagg tcaatatgcc catgtttatt ctgtcgatac cgataatttt
     3121 ggcgacagca ttgcgttgac cgatgcatta attgaaagtg ggcatcaaaa tattgcctgc
     3181 ctgcatgctc cgcttgatgt ccatgtttca gtggatcggg taaatggtta taagcagagc
     3241 ctggctgcgc ataatattgc agtgcgtgat gaattgattg ttgacggtgg ttatacccat
     3301 gaaacagcac tgcaagccgc gagagaatta ttaagccagt caccgttgcc tgaggcggtg
     3361 tttgccactg acagtctgaa attaatgagt atttatcgtg cggcagcaga gaaaaatatt
     3421 gctattccgc agcagttagc ggtggtgggt tatagtaatg aaacgctgtc gtttatatta
     3481 acgcctgcac cgggcggcat cgatgttccg acgcaggagt taggtcaaca aagctgcgag
     3541 ttattattcc gcttaatttc tggaaaaccg tcaccacaga atattaccgt tgccacgcat
     3601 atgacgctga aataaatacc agctaccttg cctgaatgcc ggatgcgatg ctagcgcatc
     3661 ttatccggcc tacatttata gatcggataa agtactcgcg ccgcatccgg caatttagcc
     3721 cattatcaga acgagtaatt tacgccgaca cctgcataat ggaatttatc actgtctcct
     3781 tcgtcgtgat cctgccactc aaacgcgtat tccagcgaaa cggaaagtcc gttctggaaa
     3841 tcataaccgt aaaataaacc tacacggtta aaatcatggc cttcacgttc aatatcatcc
     3901 tgccagtccc agttactcca gcgatccagc ccaatgcgcg tatacggcgt caccgagtgg
     3961 ttgccgagcg tcagcggcaa ataggcgcga atttcttgcg tggaaaactc accgttattg
     4021 cggctgtcgt ccatattgaa gccgcgctcg agataatagt tcactcgcaa ggcaaccgtt
     4081 tcgttgaagg tatattgcag acctgtttcc gtttcgacac gggtatcagc gtaaccggta
     4141 gtgttcagat cgttggcaaa tttatacatc gacaaccaac cgttgaaacg taaatcgtca
     4201 gtcagtttca catcccagtc tggcgcgatt ttccagcgct gcatattcgc cgtgtcttta
     4261 cccggttcat caacgtagtg ataaccataa ttacggaaac cgccggtcag gccgaaactg
     4321 aaatcatcgt tttcgaggaa ctgataatgc acctccagct ccgggcgatc aaaccacgtt
     4381 ccacgtttac ccgcgctata atctaccggc ccttcctgat aataggccag agcaattctc
     4441 cacggcccgt tggcggcatt aaaatagact gaaggctccg c
//
LOCUS       DADMKF010000228         4471 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.232, whole genome shotgun sequence.
ACCESSION   DADMKF010000228 DADMKF010000000
VERSION     DADMKF010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4471
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.232"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(<1..268)
                     /locus_tag="JNC24_003882"
     CDS             complement(<1..268)
                     /locus_tag="JNC24_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001371617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9160511.1"
                     /translation="MSGKPAARQGDMTQYGGSIVQGSAGVRIGAPTGVACSVCPGGVT
                     SGHPVNPLLGAKVLPGETDIALPAPLPFILSRTYSSYRTKTPAPM"
     gene            497..1105
                     /locus_tag="JNC24_003883"
     CDS             497..1105
                     /locus_tag="JNC24_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HAY9160512.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            1203..2594
                     /gene="selA"
                     /locus_tag="JNC24_003884"
     CDS             1203..2594
                     /gene="selA"
                     /locus_tag="JNC24_003884"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HAY9160513.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRDSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLAAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPMIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            2591..4435
                     /gene="selB"
                     /locus_tag="JNC24_003885"
     CDS             2591..4435
                     /gene="selB"
                     /locus_tag="JNC24_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HAY9160514.1"
                     /translation="MIIATAGHVDHGKTTLLQVITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPSFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPGREHASQHSVRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETAHAGQRIALNIAGDAEKEQINRGDWLLADAPPEPFTRVIVELQTHT
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQSDADALSVHLERGAVNLADFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKIRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDALLFPEK"
BASE COUNT         1002 a         1139 c         1321 g         1009 t
ORIGIN      
        1 ccatcggcgc gggcgttttt gtccggtaac tgctgtaggt gcgggagagg atgaacggca
       61 gcggggcggg cagggcgatg tcggtttcac cgggaaggac ctttgcaccg agcaggggat
      121 tgaccggatg gccggacgtc actccgccgg ggcacaccga acaggccaca ccggtggggg
      181 caccgatgcg caccccggct gaaccctgaa caatgctacc gccatactgc gtcatgtcgc
      241 cctgacgcgc tgccggtttt ccgctcatct gctcatcctt gttttatcgg gttatttgtc
      301 tgcttccagg caaaacccta cagatttata agaaagcctt aagtgagata tccatcagat
      361 agaaacaatt aggaaaatat ttatatcacc atgataaata tgtgatttat ttggaagtaa
      421 caaaccatca acattttctg ccctttttcc catcaccagg ccatactgat atttttcctc
      481 gcacaaagga ctcggcatga aactcgtcgg tagctacacc agcccgtttg tacgcaaact
      541 ttctattctg ttgttagaaa agggcataac tttcgaattt attaatgaac tgccctataa
      601 cgcggacaac ggcgtggcgc aatttaaccc gttaggaaaa gtgccggtgc tggtgaccga
      661 agagggcgaa tgctggtttg attcgccgat catcgctgaa tatattgagt taatgaatgt
      721 cgctccggcg atgttgccgc gcgatccgct ggagtcgttg cgggtgcgca aaattgaggc
      781 gctggcggat ggcattatgg atgccgggct ggtatcggtg cgtgaacagg cgcgtccggc
      841 ggcgcagcag tctgaagatg aattgttacg ccagcgggag aaaatcaacc gcagtctgga
      901 tgtgctggaa ggatatctgg tcgatggcac actcaaaacc gatacggtca atctggcgac
      961 tatcgccatt gcctgtgctg tcggatatct caatttccgc cgcgttgcgc caggttggtg
     1021 tgtcgaccgc ccacacttag tcaaactggt cgaaaacctg tttagccgcg aaagttttgc
     1081 tcgcaccgaa ccgccaaagg cttgatgcgc gatatgtcct cctgacccat ctcacgttac
     1141 aatccgtggt tatgttaaac gcccttctcc gtgtgagagg gccttgatca gccaggtttc
     1201 ctatgacaac cgaaacgcgt tccctctata gtcaacttcc ggctattgat cgcttattgc
     1261 gcgatagctc cttcctttct ttgcgtgata cttatggtca cacccgcgtg gtggaattgt
     1321 tgcgtcagat gctcgacgaa gcgcgagaag tgattcgtga cagccagacg cttcctgcgt
     1381 ggtgtgaaaa ctgggcgcaa gaagtcgatg cccggttgac gaaagaggcg cagagcgcgc
     1441 tgcgtccggt gatcaacctg acgggaaccg tgctgcatac caaccttgga cgagctttac
     1501 aggcggaagc cgcggtggaa gccgttgcgc aggctatgcg ttcgccagtg accctcgagt
     1561 acgatctgga cgacgccgga cgcggacatc gcgatcgggc gctggcgcaa ctgctgtgcc
     1621 gtattacggg ggcggaagat gcctgtatcg tcaataacaa tgcggcggcg gtgttattga
     1681 tgttggcggc cactgccagc gggaaagagg tggtagtatc tcgcggcgaa ttggtggaga
     1741 ttggcggcgc gtttcgtatt cccgatgtta tgcgtcaggc aggctgcacc ctacacgaag
     1801 tagggaccac caaccgcacg cacgcgaatg attatcgtca ggcggtgaat gaaaataccg
     1861 cactgttgat gaaagtacat accagtaact acagcattca ggggttcacc aaagcgatag
     1921 atgaagcgga actggtggcg ctcggcaaag agctggatgt tcccgtagtg actgatttag
     1981 gcagtggctc gctggtcgat cttagccagt acggtttgcc gaaagagcca atgccgcagg
     2041 agttgattgc ggcgggcgtc agtctggtga gcttctccgg cgacaagttg ttaggcgggc
     2101 cgcaggcagg gattatcgtt ggtaaaaaag agatgatcgc ccgcctgcaa agccacccgc
     2161 tgaagcgtgc attacgcgcg gataaaatga ccctcgcggc gctggaagcc acgttgcgtc
     2221 tttatttaca ccctgaagct ctgagtgaaa aattaccgac cctgcgcctg cttacccgca
     2281 gcgcagaggt cattcaaatc caggcacaac gtttacaggc tccccttgcc gcacattacg
     2341 gcgcggagtt tgcggtacag gttatgccat gtctttcgca gattggcagt ggttcgctgc
     2401 cggttgatcg cctgccgagc gcggcattaa cgtttacacc ccatgatgga cgcggtagcc
     2461 accttgagtc attagccgcc cgctggcgtg aattgccagt gccgatgatt ggtcgtattt
     2521 atgacggacg attgtggctg gatttacgct gccttgaaga tgagcaacgg tttttggaga
     2581 tgttgttgaa atgattattg cgactgccgg acacgttgac cacggcaaaa caaccttatt
     2641 gcaggtgatt actggcgtaa atgctgaccg tctgccggaa gaaaaaaagc gcggcatgac
     2701 catcgatctc ggctatgcct actggccgca gccggatggt cgcgtgccta gttttatcga
     2761 cgtacccggt catgaaaagt ttctttccaa catgctggcg ggcgttggtg gtatcgatca
     2821 cgcgctgttg gtggtggcgt gcgatgacgg cgtgatggcg cagacccgtg agcatctggc
     2881 gattttgcag ctgaccggta acccgatgct gacagtggcg ctgaccaaag ccgatcgcgt
     2941 ggacgaagcg cgtgttgatg aggttgaacg ccaggtaaag gaggtgctgc gggaatacgg
     3001 ttttgctgag gcaaaactgt ttatcaccgc agcaaccgaa ggtcggggaa ttgatgccct
     3061 gcgcgagcat ctgcttcagt taccaggacg tgaacacgcc agccaacata gtgtccgcct
     3121 cgcgatcgac cgcgcattta ccgtaaaagg tgccggtctg gtcgtcaccg gtacggcgtt
     3181 aagcggggaa gtgaaggtag gcgattcact ctggctgact ggtgtaaata aaccgatgcg
     3241 tgtacgtgcg ctgcatgcac aaaaccagcc aacagaaacc gcccatgccg ggcagcgtat
     3301 cgcgcttaac atcgcgggtg atgcggaaaa agagcagatt aaccgtggcg actggctgct
     3361 tgccgatgcg ccgccagagc cgttcacacg ggtgattgtc gagcttcaaa cccatacgcc
     3421 gctgacccag tggcagccgc tgcatattca ccacgccgcc agccacgtta cgggacgcgt
     3481 ttcgctgctg gaagataacc ttgccgagct ggtgttcgac acgccgctgt ggctggcgga
     3541 taacgatcgc ttagtgctgc gcgatatctc ggcccgcaac acgctggcgg gggcgcgtgt
     3601 ggtgatgctt aacccgccgc gtcgcggtaa acgtaagccg gaatatctgc aatggctggc
     3661 gtctcttgca cgggcgcaga gcgatgccga tgcgttatct gttcatctgg aacgcggcgc
     3721 ggttaacctt gcggatttcg cctgggcgcg ccagctcaac ggcgaaggga tgcgcgaatt
     3781 gctgcaacag cctggctata ttcaggctgg ttatagcttg ttgaatgcgc cggttgccgc
     3841 ccgctggcag cggaaaattc tcgacacatt agcgacttat catgagcaac atcgcgatga
     3901 acctggtcct gggcgcgaac gtctgcgacg tatggcgttg ccaatggaag atgaagcgct
     3961 ggtactgttg ctgattgaaa agatacgcga aagcggcgac atcctcagtc atcacggttg
     4021 gctgcatctg ccggatcaca aagcgggctt cagcgaagag cagcaggcca tctggcaaaa
     4081 agcagagccg ctgtttggtg acgagccgtg gtgggtgcgt gatctggcaa aagagacggg
     4141 taccgacgag caggcaatgc gcctgacgct acgccaggcg gcgcagcaag gaataattac
     4201 cgcgatcgtc aaagatcgtt attaccgtaa cgatcggatt gtcgaatttg ccaatatgat
     4261 ccgcgatctc gatcaggagt gtggttcaac ctgcgcggcg gatttccgcg atcgcttagg
     4321 cgtaggccga aagctggcaa ttcagattct ggaatatttt gaccgcattg gctttacgcg
     4381 tcgtcgtgga aatgatcatt tattgcgcga cgcattatta tttccggaaa aataaggaaa
     4441 tgattaatta atttttaaaa taaattaata c
//
LOCUS       DADMKF010000229         4411 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.233, whole genome shotgun sequence.
ACCESSION   DADMKF010000229 DADMKF010000000
VERSION     DADMKF010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4411
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.233"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(173..2122)
                     /locus_tag="JNC24_003886"
     CDS             complement(173..2122)
                     /locus_tag="JNC24_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAY9160515.1"
                     /translation="MADSFQLKAIITAVDKVSAPLKGMQRQLKGFKKEFASLLLGAAG
                     AGTAVLGALALPVKSAIALESKMADVRKVVDGLDTPEAFKAMTEQVRDLSTELPMSAE
                     GIAEIVAAGGQAGIARDELMQFTDDAVKMGVAFDTTAEESGQMMAQWRTAFKLTQGEV
                     AGLADKINYLGNTGPASAKKISDVVTRIGPLGSVAGVASGEIAAMGATIAGMGVESEI
                     AATGIKNFMLSLTARDSATKSQKKVLRSLRISPKKLAADMQKDARGAMLHVLDSLAKV
                     PKEKQAAVLKALFGKESLGAIAPLLTNLDLLRTNFNRVADAQQYGGSMQKEYAARAAT
                     TENQLLLLQNQINAISSTLGETFLPSLNEGIKEMKPFLEEVRTFVRENPEVVKTIAKT
                     GAALLTMGVAIGTLTRITKIMGSVMNMTPAKGLIALLVGGAYLIIDNWETVGPVVKKV
                     WQEVDQVVRAMGGWEQAVKTIATVSALYIGVKAVASIRAATVAQNQWTTAAGKTGLKL
                     KGLGKISLIGGLLELGMMAQEFEKAHPWLVKNFVADALNSGFGLNDKFDEWGKQFHDF
                     VYDMTGWQMPRGDGYLSPDKRYTPNVSLERNQLLSLASSPATRSELKVTFDNAPPGMR
                     VIDLPKTGDPFMKITHDVGYSPFKR"
     gene            complement(2207..2542)
                     /locus_tag="JNC24_003887"
     CDS             complement(2207..2542)
                     /locus_tag="JNC24_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HAY9160516.1"
                     /translation="MAMNVTEIVLKKPVTAHNEMLHVLELREPTYDEIEALGFPFIIS
                     GEGSIKLDSQVALKYIPLLAGIPRSSAAQMAKLDIFKTSMQILRFFTQSETGSTSGND
                     STMLPGSGN"
     gene            complement(2542..2898)
                     /locus_tag="JNC24_003888"
     CDS             complement(2542..2898)
                     /locus_tag="JNC24_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY9160517.1"
                     /translation="MAKIAGTCFFKVDGQQLSLTGGIEVPMNTNVRDDIVGMAGDVDY
                     KETWRSPYVKGTFKVPKNFPVDKITTSDQMTITAELANGMVYVLSAAWLHGEANHNAE
                     EGTADLEFHGEEGGYQ"
     gene            complement(2898..4394)
                     /locus_tag="JNC24_003889"
     CDS             complement(2898..4394)
                     /locus_tag="JNC24_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY9160518.1"
                     /translation="MTISFNTVPSNTLVPLFYAEMNNSAANTAVTSAPALLIGHASND
                     AAIEVNSLVLMPSADYARQICGAGSQLARMVEVYRQTDPFGELYVIAVPEARGAAATV
                     RVTVAGEAEESGTLSLYVGRSRVQVPVVNGDDATAVATAIKEAVNGVITLPFTASSDA
                     GVVTLTARHKGLYGNELPVCLNYYGSGGGEILPAGLRVVTEVGAAGSGAPDLTAAVAA
                     MGDEAFDFIGLPFNDAASINMMMTEMNDSSGRWSYARQLYGHGYTAKLGTLSELVGAG
                     DMHNQQHITLAGYEKETQSPVDELVASRLAREAVFIRNDPARPTQTGELVGMLPAPKG
                     KRFIMTEQQTLLSHGVATAYVEGGTLQIQRSVTTYKKNAYGVADNSYLDSETLHTSAY
                     VLRKLKSVITSKYGRHKLANDGTRFGPGQAIVTPAVIKGELLATYRQMERAGIVENYD
                     LFKQYLIVERDADNPNRLNTLFPPDYVNQLRVFAVVNQFRLQYSEESA"
BASE COUNT         1020 a         1315 c          970 g         1106 t
ORIGIN      
        1 aacgttacta ataaatcgtg aaacaatctc ctcatttgtt agtattttcg cctgagcaca
       61 gcaagaggtt aatattgcaa ccgtcattaa aaatggcttt atcacaaaac aaactccatt
      121 gttagcatgt tacgcgataa ttttgcctca tctttcacca caaaaaaaga actcaccttt
      181 taaatggaga atacccaaca tcatgggtga ttttcataaa gggatcgcct gttttcggca
      241 aatcgataac gcgcattcca ggcggcgcgt tgtcaaacgt caccttaagt tcgctgcggg
      301 ttgccggtga agatgcaagg cttaacagct gattcctttc cagtgataca ttcggggtgt
      361 agcgtttatc gggagataaa tacccatcac cacgtggcat ctgccatccg gtcatgtcat
      421 agacaaaatc atggaactgt ttgccccact cgtcgaactt atcattcaga ccaaatccac
      481 tgttcagtgc atcagccaca aaatttttaa caagccaggg atgtgctttt tcaaactcct
      541 gcgccatcat gcccagttca agcaatccac caatcagact gatctttccg aggcccttta
      601 atttcagccc tgtttttcct gccgccgttg tccattgatt ctgggcgact gttgccgcac
      661 gaatacttgc caccgccttc acaccaatat acagggcaga aaccgttgct attgttttta
      721 ctgcctgttc ccatcccccc attgctctga caacctgatc aacctcctgc cagacttttt
      781 tcacaaccgg accgaccgtt tcccagttat caattatgag atatgcacca ccgacgagaa
      841 gcgcaattaa tccctttgcc ggcgtcatat tcatcacgct acccatgatt tttgttatgc
      901 gtgtcaatgt gccaatcgcc acgcccatcg tcagtaatgc cgcaccagtt ttcgcgatgg
      961 tttttacgac ctcaggattt tctctgacaa acgtgcgcac ttcttccaga aaaggcttca
     1021 tctcttttat gccttcattg agtgaaggca ggaaggtttc ccccagcgtg gaagaaatcg
     1081 cattgatctg gttctgcagc agcaacaact gattttctgt cgtcgcggca cgggcggcat
     1141 attctttctg cattgagccg ccatactgct gcgcatccgc cacacgatta aagttggttc
     1201 gcaacaaatc cagattcgtg agcagcggcg caatggcccc cagggattcc ttcccgaaca
     1261 gcgctttcag cacagcagcc tgtttttctt tcggcacttt tgccagagaa tccagtacgt
     1321 gcagcatggc cccgcgggcg tctttctgca tatccgccgc cagttttttc gggctaatgc
     1381 gcaacgagcg gagcactttt ttctgcgatt tggtggcaga atccctcgct gtcagcgaca
     1441 acataaaatt ctttatcccc gtcgcagcaa tttctgactc cacccccatt ccggcaatgg
     1501 ttgcccccat tgcggcaatc tctccggagg ccacacccgc aacactgcct aaagggccaa
     1561 tacgggtcac aacatcagaa atctttttcg cacttgcagg accggtatta ccaagatagt
     1621 taatcttatc cgcaagccct gccacctctc cctgtgtcag tttaaaggca gtgcgccact
     1681 gcgccatcat ctgaccggat tcttctgccg tggtgtcaaa ggccacgccc atcttcacgg
     1741 catcgtcagt aaactgcatc agctcgtcac gggcgatacc agcctgacca cccgccgcca
     1801 caatttcggc gataccttcc gccgacatgg gcagttctgt tgacaggtcg cgcacctgct
     1861 ccgtcattgc cttaaacgct tccggcgtat ccagaccatc caccactttc cggacatccg
     1921 ccatttttga ttcaagggca atggcagatt tgaccgggag cgccagcgcc cccagtactg
     1981 cggttccggc acccgctgcg cccagtaaca ggctggcaaa ctccttttta aaacctttca
     2041 gctggcgctg cattcccttc agcggtgccg ataccttatc cacggcagtg atgatcgcct
     2101 tcagctgaaa actgtcagcc atgcttcatc tcctcgttaa tgcggacggc ctcggcttcc
     2161 agttctgtaa actgcgaaac agccccccgt cgcagctcca gtggattcag tttccagaac
     2221 caggcaacat tgtagagtcg tttccggagg tgcttcccgt ctccgactgg gtaaaaaaac
     2281 gcaggatctg catgctggtc ttaaaaatat ccagttttgc catctgcgcc gccgatgaac
     2341 gcgggatccc cgccagcaac gggatatatt tcagtgccac ctggctgtcc agtttaatac
     2401 tgccttcacc ggaaataatg aaaggaaaac ccagcgcctc gatttcgtca tacgtgggct
     2461 cgcgcaactc cagcacatgc agcatttcgt tatgcgctgt caccggtttt tttaaaacta
     2521 tttctgtaac gttcattgcc attactgata ccctccctct tcgccgtgga attcaagatc
     2581 tgccgtgcct tcttcggcat tatgattagc ctctccgtgc agccatgccg ccgaaagcac
     2641 atacaccatg ccgtttgcca gctcagcggt aatcgtcatc tggtctgagg tggtaatttt
     2701 gtcgaccgga aagtttttgg gcactttaaa cgtgccctta acgtaaggtg accgccaggt
     2761 ctccttgtaa tccacatccc ctgccatgcc gacgatgtca tcccggacat tggtgttcat
     2821 cggcacttca atgccacctg tcagcgataa ctgctgacca tccactttaa aaaaacaggt
     2881 tccggcgatc tttgccatta tgctgactcc tctgaatact gaagacggaa ctggttaacc
     2941 accgcaaaga cacgtaactg attaacataa tccggcggga acagcgtatt cagtcggttc
     3001 gggttatctg catcacgctc aactatcaga tactgtttaa acagatcgta attttccaca
     3061 ataccggcac gctccatctg acgatatgtc gccagaagtt cccctttgat aacggcagga
     3121 gtgacaatcg cctgccccgg accaaaacgg gtaccatcat ttgccagctt gtgacgtccg
     3181 tacttgcttg tgatgaccga tttcagtttg cgaagaacat atgcactggt atgcagagtt
     3241 tcactgtcca gatagctgtt gtctgccacg ccatacgcat tctttttgta ggtggttacg
     3301 gaacgctgga tctgcaacgt gccgccttcc acataagccg tcgccacgcc gtgagataaa
     3361 agggtctgct gctctgtcat gatgaatcgc ttacctttcg gtgccggaag catccccacc
     3421 agctcccccg tctgtgtcgg acgggcagga tcattccgga taaataccgc ttcacgggca
     3481 aggcgactgg caaccagttc atcgacaggc gactgggttt ctttttcgta accggcaagc
     3541 gtgatatgtt gctgattatg catatctccg gcaccaacca gctctgacag cgttcccagt
     3601 tttgcggtat agccatgccc gtataactgg cgcgcataac tccagcgacc gctgctgtca
     3661 ttcatttcgg tcatcatcat attgatggag gcggcatcgt tgaacggcag accgataaag
     3721 tcgaatgcct catcgcccat agcagcaaca gcggcggtaa gatcaggcgc tccgctaccg
     3781 gcagcaccga cttccgtcac cacccgaagc cctgctggca gaatctcacc accaccagaa
     3841 ccataataat tcagacagac cggtaactca ttaccataca gccccttatg acgggcagtc
     3901 agcgtcacca cacctgcatc agatgacgcc gtaaacggca gggtgataac cccgtttacc
     3961 gcttccttaa tcgcggtggc aaccgcagtg gcatcatcgc cattcaccac aggcacctgt
     4021 acacgggagc gcccgacata cagactcagg gtgccgcttt cctctgcttc tccggcaacc
     4081 gtcaccctga ccgtcgccgc cgcccctctg gcttccggta ctgcaataac atacagttca
     4141 ccgaaaggat ctgtctgacg gtagacttcc accatacgcg ccagctggct ccccgcccca
     4201 caaatctggc gggcataatc tgccgatggc atcagcacca ggctgttaac ctcaatggct
     4261 gcatcgttgc tggcatgccc gatcagtaat gcaggcgcgc tggtcaccgc tgtatttgcc
     4321 gcagaattgt tcatctcggc gtagaacaac ggcaccagcg tattcgacgg aacagtatta
     4381 aaacttatcg tcatgatttt ttagccttat c
//
LOCUS       DADMKF010000230         4404 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.234, whole genome shotgun sequence.
ACCESSION   DADMKF010000230 DADMKF010000000
VERSION     DADMKF010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4404
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.234"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(139..3654)
                     /locus_tag="JNC24_003890"
     CDS             complement(139..3654)
                     /locus_tag="JNC24_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase"
                     /protein_id="HAY9160519.1"
                     /translation="MDKNALGFASYWRNSLADAESGKGSFERKDAKNFTHWHGIAAGR
                     LDEAIVSKFFKGEKDDVETVDVILRPKVYFRLLQHGKDRSAGAPDIVTPIVTPALLSR
                     EGFLYPTPATSIPRDLLEPLPKGAFSIGEIGQYDKYKTTHTTFSINFDDSVDKTAETD
                     EEREARYAALQQEWRQYLYDSERLLKSVAGDWIEKPEQYELAEHGYIVKTAQSGGASS
                     HILSLYDHLIVCNKDVPLFNRFASREVHAAESLLAPGAKFSDRLGHSGDKFPLAKAQR
                     DALSHFLDARHGDILAVNGPPGTGKTTLVLSIIATQWARAALEKSEPPVIIATSTNNQ
                     AVTNIIEAFGKDFSQGSGAMAGRWLPELKSFGAYFPSSSRKAEAAKKYQTEDFFNQVE
                     SKEYVEDALLFYLEKAKAAFPGKECSSPEKVIELLHGQLAAKSEQLIRLNATWQTLSQ
                     IRAARELIANDIEQYLDNLNKLLSGQEQKVTLLKSAKTEWKKYRAGESLIYSLFSWLP
                     AVRNKRQYQIQLFLEDKLGALIAGNQWSDPETIERNIDGLLNSAEREQTTYRQQIDSA
                     HEIVLKEQQAVQEWQRLAFDLGYEGDEELSFSQADELADTQIRFPAFLLTTHYWEGRW
                     LMDMASIDDLQDEKKKKGAKGVTARWQRRMKLTPCVVMTCYMLPGNMQISEHKGQRKF
                     EKSYLYDFADLLIVDEAGQVLPEVAAASFALAKKALVIGDTEQIPPIWSIAPAIDVGN
                     MLAEKILSGSTQEEITEKYTAIADLGKSAASGSVMKIAQFASRYQYDPELARGMYLYE
                     HRRCYDNIIGYCNTLCYHGKLLPKRGREESNLMPAMGYLHIDGKGELASSGSRYNLLE
                     AETIAVWLAENQQNIEAHYGKSLHEVVGIVTPFSAQVSTIKQVLGKQGISTGTNEKSL
                     TVGTVHSLQGAERAIVIFSPVYSKHEDGGFIDSDNSMLNVAVSRAKDSFLVFGDMDLF
                     EVQPASSPRGLLAKYLFESEKNALSFDYKERKDLKTAGTKIYTLHGVEQHDNFLNQTF
                     ENTSKHITIISPWLTWQRLEQTGFLDSMIAACSRGINVTIVTDRSYNTEHNDFEKRKE
                     KQQNFKAALEKLNALGIATKLVNRVHSKIVIGDDGLLCVGSFNWFSATREARYERYDT
                     SMVYCGDNLKGEIEAIYNSLERRQV"
     gene            complement(4106..>4404)
                     /locus_tag="JNC24_003891"
     CDS             complement(4106..>4404)
                     /locus_tag="JNC24_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HAY9160520.1"
                     /translation="VNKALRVMGYDTTQDVCGHGFRAMACSALIESGLWSRDAVERQM
                     SHQERNGVRAAYIHKAEHLEERRLMLQWWADFLDANRDKGISPFEYAKINNPLK"
BASE COUNT         1141 a         1128 c          905 g         1230 t
ORIGIN      
        1 gagcctctga ttcttccgga cagaagataa tcatgtcggt atgaatgtta taattaagga
       61 ctgtgcaata atagccattc cattcagcca tcttacgctc gaaaacgatc ggtaagatgg
      121 ctactcacca ttcctgctct aaacctgacg cctctcaaga ctattataaa tagcctcaat
      181 ctcacccttc aggttatcac cgcaataaac cattgatgta tcgtatcgtt catatcgcgc
      241 ttcccgtgtc gcactaaacc agttgaacga tcccacacac agcaaaccat catcaccaat
      301 aacaattttg ctatgaacac ggtttaccag ctttgtagca atacccagcg cattcagttt
      361 ctccagcgcc gctttaaagt tctgctgctt ctcttttcgc ttctcaaaat cattatgttc
      421 agtgttgtag cttctgtcag tgactatcgt gacgttaatt ccacgtgaac acgccgcaat
      481 catggaatca agaaaaccgg tttgctccag cctttgccag gtcagccatg gagaaattat
      541 cgtgatgtgt ttactggtat tttcaaatgt ctgattcagg aaattatcat gttgctccac
      601 accatgaagt gtgtagattt tggtcccggc ggtttttaaa tccttacgct ctttataatc
      661 aaaagagagc gcattcttct ctgactcaaa gaggtatttt gccagtaatc cccgtggcga
      721 tgaggctggc tggacctcaa acaggtccat atcgccgaag accagaaaac tgtccttcgc
      781 acgggagact gcaacattca gcatgctgtt atcgctatca ataaacccgc cgtcttcatg
      841 ttttgaatag actggcgaga atatcacaat cgctctttcc gctccctgaa gagagtgcac
      901 ggtgcccact gtgagcgact tttcattcgt gcctgtactg ataccttgtt tgcccagcac
      961 ctgtttgata gtggatacct gagcgctaaa aggcgtcaca ataccgacaa cttcatgaag
     1021 cgatttaccg taatgcgctt caatattttg ctggttctct gccaaccaga ccgctatcgt
     1081 ttcagcctca agcaaattat atcgacttcc actacttgcc agctctcctt taccatcaat
     1141 atggagatac cccattgcgg gcattaaatt gctctcttca cgccctcttt taggcaacaa
     1201 cttaccgtga tagcagagcg tattacagta tccaataata ttgtcgtagc accggcggtg
     1261 ttcatatagg tacataccac gagccagttc gggatcatat tgatagcgcg aagcaaactg
     1321 cgctattttc ataacgctgc cagatgcggc acttttacca aggtctgcga ttgccgtata
     1381 tttctcggta atctcttctt gcgtactgcc agacagaatt ttttccgcca gcatgttacc
     1441 gacatcaatc gcaggagcaa tactccatat tggcgggatc tgctccgtat cgccaatcac
     1501 taatgccttc ttagctaatg caaacgaggc agcagccact tcaggaagca cctgcccggc
     1561 ttcatcgaca atgagtaaat cggcaaaatc atacaaataa cttttctcga atttacgttg
     1621 tcctttgtgc tcacttatct gcatattacc gggcagcata tagcatgtca ttaccacaca
     1681 tggcgtgagt ttcattcgac gttgccaacg ggcggttacc cctttagcac cttttttctt
     1741 cttctcgtcc tgcagatcat caatgctggc catatccatc agccaacgac cttcccagta
     1801 gtgagtcgtc agtaaaaatg cagggaagcg aatctgcgta tcagccagtt catcggcctg
     1861 tgagaagctc agttcctcgt cgccctcata ccctaaatca aatgccagcc tctgccactc
     1921 ctgaaccgcc tgctgttctt taagaacgat ttcatgggcg gagtcaatct gctgccggta
     1981 tgttgtttgc tcgcgctcag cggaattgag cagcccatca atattacgtt cgatagtttc
     2041 aggatcagac cactgatttc ctgcaatcag cgcgcctaat ttatcttcga gaaacagctg
     2101 tatttggtac tgtcgcttat tgcgaaccgc cgggagccag gaaaataatg aatagatcag
     2161 tgattcaccg gcgcgatatt ttttccattc cgttttagca ctcttcagta gagtgacttt
     2221 ttgttcttgt ccggaaagta atttatttaa attatcgaga tattgctcaa tatcattagc
     2281 aataagctca cgcgcagccc gaatctggct taacgtttgc catgttgcgt tcagtcttat
     2341 cagttgctca gattttgctg ccaactgacc atgcaggagt tcaatgacct tttcagggga
     2401 tgaacactct tttccaggaa aggctgcctt agccttttcc agataaaaca gcagtgcatc
     2461 ctctacatac tcttttgact caacctggtt gaagaaatct tcagtttgat attttttggc
     2521 tgcctcagct ttacgactgc ttgagggaaa ataagcaccg aagcttttca gctctggcaa
     2581 ccatcgcccg gccatcgcac ctgaaccttg cgaaaagtct ttcccgaatg cctcaataat
     2641 gttcgttaca gcctggttat tcgttgaagt cgcgataata accggaggct cagatttttc
     2701 gagagccgct ctggcccact gcgtggcgat gatagaaagc accagcgtgg tttttccggt
     2761 tcccggaggg ccattaacag caaggatatc gccatgtctt gcatccagaa aatggcttaa
     2821 ggcatcgcgc tgagcctttg ccagcggaaa cttatctccg gagtgtccaa gcctgtcgct
     2881 gaattttgct cctggggcca gcaaagactc tgcagcatga acctctcgcg aggcgaagcg
     2941 attgaagagc ggcacatcct tattgcaaac aatcaggtga tcataaagag aaaggatatg
     3001 ggaactggca ccgccagatt gagccgtttt aacaatataa ccgtgctcag cgagttcata
     3061 ttgctcaggt ttttcaatcc agtcgccggc aacgctcttc agtagcctct ctgagtcata
     3121 cagatattga cgccactcct gctgcaaggc ggcatatcgt gcttcccgtt cttcatccgt
     3181 ttcggcagtc ttatcaacgc tgtcatcaaa gttgatagag aacgtggtat gggtcgtctt
     3241 gtatttgtca tactgcccaa tctcaccaat cgaaaatgct ccttttggca aaggttcaag
     3301 caggtctctg ggaatggagg tcgctggcgt cggatataaa aaaccctcac ggcttagcaa
     3361 ggctggcgtc actatcgggg taacaatatc aggcgcacct gcagaacggt ccttaccatg
     3421 ctgcagtaac cggaaataaa cttttgggcg caagatgaca tcgaccgttt cgacatcgtc
     3481 tttttctccc ttaaaaaatt tactgacaat cgcttcgtca agacgtcccg ccgctatccc
     3541 atgccagtga gtgaaatttt tggcgtcttt ccgttcaaaa ctgccctttc ctgactcagc
     3601 atccgcaagc gagttgcgcc agtatgaggc aaaccctaaa gcatttttat ccattaccag
     3661 tcctgtgact caatgaaatc cattgattta tcgaatataa aaccaccata tggacaacta
     3721 ttgtccatat tgaaaaagtt ggatgcaatg ttttgtttat aattcggtat caaatgtact
     3781 ggttgtacaa ggttcagaac acaagcacat tcaacagtac gtcaggacta ttgatttcat
     3841 aacagtgaat acatttttta ccgtttataa tgactgcctg tgttggacaa ggataccaga
     3901 acagccgaaa acaccggggg ttattacctg ctctcatgga aaccagatat tcctggtgct
     3961 aatcacagtc ataaatagcc tgaagaacat gacgaaggga ctttaatgga agccggagta
     4021 acttgccaga acatcaatct tcgtcatcat ttacaaagaa aaataatcag ttaaagcagt
     4081 ccgggcaatc agcccggact gcttactatt ttaatggatt gttaatcttt gcatattcaa
     4141 acgggctgat acccttatct ctgttcgcat ccagaaaatc tgcccaccac tgtagcatca
     4201 gtcgacgttc ttccagatgt tctgctttat ggatatacgc agcacgtaca ccattacgct
     4261 cctgatggct catctgacgt tccacagcat cgcgggacca caaacctgat tcaatcaatg
     4321 cactgcacgc catcgcccgg aatccatggc cacagacatc ctgggttgtg tcatacccca
     4381 tcaccctcag ggctttattt acag
//
LOCUS       DADMKF010000231         4401 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.235, whole genome shotgun sequence.
ACCESSION   DADMKF010000231 DADMKF010000000
VERSION     DADMKF010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4401
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.235"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(<1..590)
                     /locus_tag="JNC24_003892"
     CDS             complement(<1..590)
                     /locus_tag="JNC24_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000785587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate protein"
                     /protein_id="HAY9160521.1"
                     /translation="MADSQFYRPGLPQLISMIRSDLLTRFEQDTLLRRMDAEVYARVQ
                     AAAVHTLYGYIDYLARNLLPDVCDEDWLYRHARIKRCPRKEAVAARGFVRWDGVEGTP
                     VLPAGTQIQRDNQVTFTTTATVTAADGLLRVPVVADEPGSAGNTDDGIAMQLGTPVSG
                     LPSTGYADTITDGEDIENLEIWRARVMERYYYIPQGG"
     gene            complement(583..993)
                     /locus_tag="JNC24_003893"
     CDS             complement(583..993)
                     /locus_tag="JNC24_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000973352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9160522.1"
                     /translation="MMINVNGRPVSTGASIDLLTRAVIISLFTWRRAGRDDAPRIFGW
                     WGDTWPAVQNDRTGSRLYLLRRSKLTNKTPQLARDYVREALAWMVEDGVASRLDINAV
                     RTGTDSLALAITIYQRDGNIHNIIFDDIWSELNG"
     gene            complement(999..1532)
                     /locus_tag="JNC24_003894"
     CDS             complement(999..1532)
                     /locus_tag="JNC24_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015967647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein"
                     /protein_id="HAY9160523.1"
                     /translation="MNGFSLRNLISRAVITAVDSARKCQSVGLKMIAGDQKQHVEHLE
                     PYGFTSAAQNGAEGVALFPAGDRSHGVVVVVADRRYRLKGLKRGEVALYDDLGQSVVL
                     TRSGIVVDGAGKAIIFKNAPKARFEMPVESTADITDNCDSGGLSMQQMRQAYNAHKHT
                     ENGDGGGITDTPDQPMG"
     gene            complement(1510..2592)
                     /locus_tag="JNC24_003895"
     CDS             complement(1510..2592)
                     /locus_tag="JNC24_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001343745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate protein"
                     /protein_id="HAY9160524.1"
                     /translation="MNDTVLLRVSGREWGGWTSVRISAGINRIARDFNVAITTRWPGS
                     RDYQPRIKNGELVEVLIGDEPVLTGYVEALPLRYDASSVSMGIVGRSKTADLVDCSAL
                     PLQQSGKNLLRIVSELAAPFGITVVDAGVPQTAVIDAQPEHGETVADCLNRLLGQVQT
                     LAYDDECGRLVLGKPGTGKAATALVLVLGENILSCDTERSIRERFSEYQVSGQRPGND
                     DDFGEATIAAIRQTIQDSGVTRYRPLLIQQSGTATTATCKARCEFEARQRAALTRETT
                     YTVQGWRQGSGALWRPGLSVIVFDPLNNFDNDELVIAEVTYNQDDRGTTTELRVGPAD
                     AYLPEPVTARKKKMLRRISDERVFSS"
     gene            complement(2589..3980)
                     /locus_tag="JNC24_003896"
     CDS             complement(2589..3980)
                     /locus_tag="JNC24_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000347288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA circularization protein"
                     /protein_id="HAY9160525.1"
                     /translation="MDLPSLPSLSSLFSASSGINWRDNLYDASFRGVPFSVESDEGSF
                     GRRVQVHEYPNRDKPYTEDLGRATRRLTINAYLVGDDYAEQRDRLITAIETAGPGTLI
                     HPQFGEMQGCIDGQVTVSHSGTEGRMCRVSFQFVESGELSFPVAGAATARKLEESSVF
                     LDELIEDMFGDFDLAGIPDFIQNDVIARTTDMLGTVQTAFKMVNSAVSAGARLLQGDL
                     SVILMPPSVASDFVHTLQDTWRAGTRLVDNTQDLVQSITTMSGITLDPGLAPRAVWPT
                     DSASVIRQKQQTNLVAAVIRTTAISEATRAVTSLPQPGSLVKNQQAVVAVGGSTERQS
                     DIIHVSHPALDSVAASTGQDERAQPPTRENLTIIRKSLNAAIEQELRRTADDRLFFQL
                     TSLRTELNRDIQARLVQTEETAERTPAEVLPALVLAASWYDDASRETDILDRNAISHP
                     GFVPVRALRVPVR"
     gene            complement(4027..4233)
                     /locus_tag="JNC24_003897"
     CDS             complement(4027..4233)
                     /locus_tag="JNC24_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001343746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9160526.1"
                     /translation="MTFLIPIYKDDDFDSDTVGFTFAFKMPRGQFFVDVKENGNIRAG
                     VNVNGESGVTYENCKLNMKDINDD"
BASE COUNT         1054 a         1283 c         1040 g         1024 t
ORIGIN      
        1 cccccctgtg gaatgtagta ataacgttcc ataacgcggg cacgccatat ttccagattt
       61 tcaatatctt caccgtctgt aatggtgtca gcgtaccctg tggacggcag accactgacg
      121 ggtgttccca gttgcatggc aataccatca tccgtattcc ccgccgatcc cggttcgtct
      181 gccacaacag gcacccggag aagaccatcg gctgcggtca ccgtcgccgt cgtggtaaag
      241 gtcacctgat tatcacgctg gatctgcgtt cccgctggca ataccggcgt cccctctacg
      301 ccatcccagc gcacaaatcc ccgggctgcc accgcttctt ttcgcgggca gcgtttgatt
      361 ctggcgtgcc ggtacagcca gtcttcatca cacacgtccg gtaacagatt tctggcaaga
      421 taatcgatat acccgtacaa cgtgtgtacg gctgcagcct gtacacgggc atacacttcc
      481 gcatccatac gacgaagcag cgtatcctgc tcaaagcggg ttaataaatc gctccggatc
      541 atagaaataa gttgcgggag gccggggcga taaaactgac tgtcagccat tcagttcact
      601 ccagatatca tcaaaaataa tgttgtgaat attgccgtca cgctggtaaa tggtaatggc
      661 aagtgccagc gagtctgtcc cggtccggac agcgttaata tcaagacggg aagcaacacc
      721 atcctccacc atccacgcca gcgcctcacg gacataatct ctggcaagct gcggggtttt
      781 atttgtcagc ttgctgcgtc gcaacagata caaacgcgac cccgtgcgat cattctgaac
      841 cgcaggccag gtatcccccc accatccaaa tatccgtggt gcatcatcac gcccggcacg
      901 ccgccatgta aaaagcgaaa taatcacagc acgcgtcaga aggtcgatcg aagccccggt
      961 cgacacgggt cgcccgttaa cattaatcat catgattttc agcccatcgg ttgatccggc
     1021 gtgtcagtga tcccgccacc atcaccattt tcggtatgtt tgtgggcatt gtaggcctgc
     1081 cgcatttgct gcatgctgag tccgccactg tcgcaattgt cagtaatatc ggcggtggat
     1141 tcgactggca tttcaaagcg cgctttaggc gcgtttttaa aaattatggc cttcccggcc
     1201 ccgtccacca caataccgga acgggtcagg acaacggact gtcccagatc gtcataaagc
     1261 gccacttccc cacgtttcag tcctttcagc cggtaacgtc tgtcagccac gaccacaacc
     1321 acaccatgag aacgatcgcc cgccggaaat aaagcaacgc cctcagcacc gttctgtgcg
     1381 gcagatgtaa aaccataagg ttcaaggtgc tcaacgtgtt gtttctgatc tccggctatc
     1441 attttcaacc ctacagactg acactttctg gcggaatcca ccgccgtgat gacagcccgg
     1501 gaaatcagat tacgaagaga aaacccgttc atcagaaatc ctcctcaaca tttttttctt
     1561 cctggcggta acaggctcgg ggagataagc atctgccggg ccaacccgta attcagtcgt
     1621 cgtgccccgg tcgtcctggt tataggtaac ttctgcgatc accagttcat cattatcaaa
     1681 attattcagc gggtcgaaaa cgatgacaga taaccccgga cgccataacg cgccactgcc
     1741 ctgccgccag ccctgaaccg tatatgttgt ctcacgggta agcgcagccc gttggcgcgc
     1801 ttcaaattca caacgggcct tacaggttgc tgtcgttgct gtgcctgact gctgaatcaa
     1861 caaagggcga taacgggtca cgccactgtc ctgaatggtc tgacgtattg cggcaatggt
     1921 ggcctcacca aaatcatcgt cgttgcccgg gcgctgccca ctgacctgat attcagaaaa
     1981 ccgctctctg atgcttcttt ccgtgtcaca ggaaagaata ttctctccca gcaccagcac
     2041 cagcgccgtc gccgctttac ctgttccggg ttttcccaga accagtcgcc cgcattcatc
     2101 gtcataagcc agcgtctgaa cctgccccag taaccggtta agacaatcgg caacggtttc
     2161 gccgtgttcc ggctgggcat caatcaccgc tgtctgcggc acgccagcat caacaacggt
     2221 gatgccaaat ggcgcagcca gttcactgac tattctgagc aggttttttc cgctctgctg
     2281 gagtggcaaa gcagagcagt caaccagatc ggctgttttg cttcgcccga caattcccat
     2341 gctgacgctg ctggcgtcat aacgaagcgg tagtgcctcc acatatccgg tgagcacagg
     2401 ctcatccccg ataagcactt caaccagctc accatttttt atccggggtt gataatcccg
     2461 gcttccgggc cagcgggtgg taatggcaac attaaaatcc cgggcaatac ggttaatgcc
     2521 cgcactgata cggacggatg tccagccgcc ccattcgcga ccggaaaccc gaagtaaaac
     2581 ggtatcattc atctgacggg tacccttaat gccctgaccg gaacaaagcc cggatgagag
     2641 atggcatttc gatccaggat atcagtttca cgggacgcat cgtcgtacca tgacgcagcc
     2701 agaacaagcg caggcagaac ttccgctggc gttcgctctg cggtttcctc cgtctgaacc
     2761 agacgcgcct gaatatcccg gttcagttct gtacgtaatg acgtcagctg aaaaaacagc
     2821 ctgtcatccg ccgtacgcct gagctcctgt tcaatcgccg cgttcagcga tttgcgtatg
     2881 atggtgagat tttcccgcgt gggtggttgc gctctctcat cctgtcctgt gctggctgcc
     2941 acgctgtcaa gtgccggatg agaaacgtga ataatatcgg actgacgttc agtggaacca
     3001 ccaacagcca caaccgcctg ctgatttttc accaggcttc cgggttgtgg cagtgaagtg
     3061 accgcccttg tggcttcgct gattgccgtc gtccggatga cagcagccac aaggtttgtc
     3121 tgctgttttt gcctgataac cgatgcggaa tctgtgggcc acaccgcacg cggtgccagt
     3181 cctgggtcca gcgtaatgcc ggacattgtc gttatggact gcaccagatc ctgtgtgtta
     3241 tccaccagcc gggttccggc ccgccaggta tcctgcaacg tatgcacaaa atcactggca
     3301 accgacggcg gcatcagaat gacggataaa tcgccctgca gtagtctcgc tccggcagaa
     3361 acagccgaat taaccatttt gaaagctgtc tgaacggttc ccagcatatc ggtcgtccgg
     3421 gcaataacat cgttctgaat aaagtccggt attcccgcga gatcaaaatc gccgaacatg
     3481 tcttcaatca actcatccag gaatacggac gactcctcca gttttctggc ggttgcagct
     3541 ccggcgaccg gaaatgacag ttccccgctc tcaacaaact gaaatgaaac ccggcacata
     3601 cggccttcag tgccggagtg agaaacggtc acctgcccgt caatacagcc ctgcatttca
     3661 ccgaactgcg gatggatcag cgtccccggc ccggcggttt caatcgctgt aatcagcctg
     3721 tcgcgctgct ctgcgtaatc atcaccaacg agatacgcat taatcgtcag ccgtcgcgtg
     3781 gcacgcccga gatcttccgt gtacggtttg tcacggtttg ggtattcatg gacctgaacg
     3841 cggcgtccga acgaaccctc gtcgctctcc accgaaaacg gaacgccacg aaatgaagcg
     3901 tcgtaaagat tatcgcgcca attgatacct gaagatgcag aaaagagtga agaaagagaa
     3961 ggtaaagaag gtaagtccat actgtctcca ctataaaaaa accgccaatt tctggcggct
     4021 tatatattaa tcatcattaa tatctttcat attcaatttg caattttcgt aggtcactcc
     4081 cgactcgcca ttaacattaa ctccagctct gatattcccg ttttctttta catctacaaa
     4141 aaactggcct cttggcatct tgaacgcaaa tgtaaaacca acagtatctg aatcaaaatc
     4201 atcatcttta tatattggga tcaaaaatgt catcgtcgct ggtgatgcat tgtttgcaaa
     4261 atcaaataca cctatacttt tgccgaattc ataagaagct tttctcaata taatcgcacc
     4321 gctggcagaa ggggatgggc acgatatcct cagttgtctg aatccatctg tatccaagtc
     4381 aacgtcatta tctgcagatg t
//
LOCUS       DADMKF010000232         4330 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.236, whole genome shotgun sequence.
ACCESSION   DADMKF010000232 DADMKF010000000
VERSION     DADMKF010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4330
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.236"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(<1..583)
                     /locus_tag="JNC24_003898"
     CDS             complement(<1..583)
                     /locus_tag="JNC24_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAY9160527.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIDLGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTIISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADAITVDAMIPNF"
     gene            complement(646..1095)
                     /locus_tag="JNC24_003899"
     CDS             complement(646..1095)
                     /locus_tag="JNC24_003899"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HAY9160528.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVETDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            complement(1092..4184)
                     /gene="ebgA"
                     /locus_tag="JNC24_003900"
                     /pseudo
     CDS             complement(1092..4184)
                     /gene="ebgA"
                     /locus_tag="JNC24_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026199.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
BASE COUNT         1029 a         1090 c         1168 g         1043 t
ORIGIN      
        1 tgaagttcgg gatcatcgcg tcaacggtga tggcgtcagc aggctgtacg ccgccaacca
       61 gcgccgtacc tgccagtaaa atgtaggaga gcgttaacag cagcatcagc gttgaagtga
      121 cggaggtaat tggccccaac attttcgccc cgttggtgga aacccaggtg gagaaggcga
      181 acagcaccat actgataatg gtggtggcaa ccggcgtcat aatatattcg tatccgagga
      241 aggcatacga agcataagca ataacgcgcg gcaacagtga ggtgaaaaag aacaggttta
      301 cgaaccagta ggtataggca gtaataaatg cccaacgtcc gcccagcgaa ctttttaccc
      361 atgcgtagac accggcttct gagtttttat ttaacgaaac aaattctgcg atgatcagac
      421 agaagggaat aaaataaaaa atcgtcgcga ggaaaaacat cggtgccgag gccagtccaa
      481 gatcaatatt attattgata acgttgttaa agctgtaaac ggcggcaaaa gtcagcgaga
      541 gcaagccgaa tttgccgatt gtattacgtt tggtatcaga cataacacat ctccgaaaat
      601 gagtagggta gcggtggcgg caaactcctt ttgccgccgt agttgttatt tgttatggaa
      661 ataaccatct tcgatggtga ctttgagaac cacttttttg accgcgttat tgcagataaa
      721 ccgatacgct tcatggatat cgcaaataac gatttgccct tcgtggacct caacggtttc
      781 tccgcagcct tttaaatatt cacggtcagt ttcatcacga taatattcca ctacctgtaa
      841 tgtctctttc ggcgcatatt caattttttg ctgcccttgc aggtagtaat gcacttcaaa
      901 atagcgtcga tgcccggtaa atagcgcatc ggtcgcggag tctgtctcca cgcggtaagt
      961 caatgagtca ccgatggagt gggcgacgcc aggctgaatg ttgtcgatat tttcaatcgc
     1021 ttcaacgcag cgttgccact tcttgccaga ggcgtaaatc tggcggaact gttctaagtt
     1081 atcgatgatc ctcattgctt attctcgctg tgcaaattcg tggaaaagaa ccctgcgccg
     1141 aactcatacg acgccaggct ttgcgcggta gcttctccgc cagaaaccgg cagcaacgta
     1201 aagccgtagc tgaagtcacg gaaccagacg cgccaggagt ccagcacctc gctgccccag
     1261 gagttggagc cgaggccaag cagctggtga tcgaggttca gggtgatgtc atcactgcgc
     1321 tgcagctcgt tacagtgctg ggcagcgtgg atgttttcct aggtatagtg ccaggcgctg
     1381 aagttaattg ggcgctgcgg aaccaccagc agaccgttac cgtggcggtt agtcagtgcc
     1441 gtccagcgga catgctgacg gttaccgttg ttctgcggga agggatagtt ctcgaacatg
     1501 gcatcgacgg tgctgcgcca gatatcgatg atgttagcct gctggctgtc ggcgtagttt
     1561 tcgcccggtc cacgaccgta atacgccacc tgatcgtatt cgccgttaat tcccatggtg
     1621 aaaccgatgc acggaatgat gtgcggatag tcgccgtaac gctcgccgga aagcgccacg
     1681 ttaacctggc catcggcagc gatgcgccag atgtaggtgc agcgcattcc gaaatcaaac
     1741 accggcgggg caataaccgt gcggctgatg atcaacactt caccctcgct ctgttctacg
     1801 gcaaagtcgc gcagatgttc ctgcatgatc tgcaaatgat tcggctgcca caacccttcg
     1861 tactcctgct tgtggttgtc gatcatcggc ttgaagaagt tgatctttgg ctcgcgggtc
     1921 agcagcgact cgccatttac ctgccaggat gtcggtttgc cactcatttt tgagaaggtg
     1981 atcgcgaagt tgtagccgcg aacggtgcag ctcaaacgat cgtcttccag cgtcagcgga
     2041 cgcgcattat ttggtgcgaa aggcactggc tgcgcggtgt tttccttcag cgggaactga
     2101 taagtggcga tagaatgccc ggcttcgctg tagcgggtgc gggaatcttt ggtcaccgta
     2161 atgttgagga acgcttcgcg ggcgtccagc tgcggcagcg tgatctgcaa gggggcttcg
     2221 ctgttcggcg caacgtcgcg cagtttaatc tgctgcgtcg cgagcgtttc accttcggcg
     2281 cgcacctctg cgtgcagggg gtagtcatca agcgtggtaa accacagttt attttcgact
     2341 ttcaactcgc cgcgagtcag atcccgcgcg tggattttta ccggcgcgat aacctgtttg
     2401 tactctttca ggcccggtcc cggcgtctga tcggaataga tcaaaccatc aagacagaag
     2461 ttatagttgt tgggatagtc gccgtagtcg ccgccgaatt tataccagac attgccgtgg
     2521 tcgtcctgtg cctggatccc gtggtcgcac cactcccaga cataatgacc ctgaatgcaa
     2581 tcgtgcttat agaagacgtt ctggtactcc gtcagcccgc ccggtccgtt ccccatcgca
     2641 tgagcatatt cacagatgat gcgcggcttc ggatgcgggt attcaccaaa ctcattcatc
     2701 agcggcacgc gggtgtacat ggtggaaata atatcgacca cttcagcatc gcgatcttct
     2761 tcgtaatgca ccagtcgcgt gtcatccagc gccttcgctg cgtggtacat cgcgcggatg
     2821 ttacagccat agccggattc attgcccagc gaccagatga tgatcgacgg atggtttttc
     2881 tgcgcgtgaa tatggcgaac aatgcgctcg acgtagactt tttcccactg cggatcgtcg
     2941 gtaatacggc tgatatcgcc gacattagca aagccgtgcg attcgacgtc ggtttccgcc
     3001 atcacaaaca ggccgtagat atcacacagt tcgtaaaaac gcggatcgtt cgggtagtga
     3061 gcggtacgca cggagttgat gttgtgctgc ttcatcaact ggagatcttt ctcgacgcga
     3121 tccattccaa cggcgcggcc tttgcgatga tcgttgtcgt gacggttgac gccgtgcagc
     3181 atcacataac ggttattgat ccagaacaga ccgtcgcgca ctttgatatc acggaagcca
     3241 acgcgttgtg gtaccacttc cagaacgttg ccgttggcgt ctttcagcgt catgaccaga
     3301 tggtaaagat aaggggattc tgctgaccat tgctgcggct gttcgacagt aaaagcaaag
     3361 ctggcgctgg tcagtttttc aattgccaaa tgatcaatgg cgctgctgtg caccacgcgt
     3421 tcgccatcaa acagggtata ttccagcgtc gtgacgacag gggaggcggc gagattttcc
     3481 agcaccactt cgcaggaaag cgtggcatcg caataggctt cgtcaaagtc ggtacgcacg
     3541 gtgaaatcgt taatatgtgt tagttgtttt ccgatcagat aaacatcgcg gaagatcccc
     3601 gctgaccacc acatatcctg gtcttccacg taggtagagt ccgcccattg catcacgcgc
     3661 acacacaaca ggttgtcgcc ggttttaacc atcgcgctga tgtcaaactc tgcggtcagg
     3721 cgactgccct tgctgaaacc cacatactga ccgttaacgt agacttcaaa ataggtttcg
     3781 acgccgtcaa atttaatcag cgtctgttta ccctgccagc cgtcgctgag ggtgaaaata
     3841 cgttgatagg caccggttgg gttatcgctg ggaacaaacg gcacatcgat ggggaacgga
     3901 aaaccttcgt cggtatattg cagtttgccg tgaccttcca tttgccacat ggcggggacg
     3961 gtaatatgcc cccagtcagc cattaactca gaggtgaagg cttcaggcac ttgcagcggg
     4021 tggtcaaaaa agtggaaatt ccactgaccg cttaagggca gaaacaggct gctggtttcg
     4081 cgggcaaagg tacgcgcttg cgcaacagaa tcatatgaaa aaaagtacgc acgtggcgca
     4141 agtcggtttt cgtgggtgag ctgaatgttt tcccagcgat tcatagggcc tcccggtaga
     4201 gagaaatcgg aaccattcat ggtgatggta gtttgtgtaa ttgctgggga gtttagtaaa
     4261 atttttacta aaaagtgaca ggtaaatgca tttactttaa ctggatcacg aaaataaacg
     4321 gcagcactgt
//
LOCUS       DADMKF010000233         4265 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.237, whole genome shotgun sequence.
ACCESSION   DADMKF010000233 DADMKF010000000
VERSION     DADMKF010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4265
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.237"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(289..630)
                     /locus_tag="JNC24_003901"
     CDS             complement(289..630)
                     /locus_tag="JNC24_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000578010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9160529.1"
                     /translation="MIFTKFQSLTHKIDTMIIHDIKREMPLKYGLYRVAKWFAWLAHT
                     GIFCTFIIYIGFSIITQHAGQELPETFKHGFALTFCSFATAALVSQWIGGGLHSKLEE
                     RIRMKWQNHAH"
     gene            930..1517
                     /locus_tag="JNC24_003902"
     CDS             930..1517
                     /locus_tag="JNC24_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HAY9160530.1"
                     /translation="MLIKNPISYFHIGTFLFSGKKRHLLEYWKAEVIMRDKFVFHRLI
                     RNGMQRQRGFLLWWRLANEMYILGNKKQRKCAIKIKNTLMAKYGCDIGLGAQIGKGLV
                     LPHHSGVVIHGNVKIGENVIIRQNTTIGEKESDSRENYIVIGDNVDIGAHTCIIGLNV
                     KIGSNVKIGAMSFIMEEVPDNCTYVTRKESRVIMR"
     gene            complement(1799..2392)
                     /locus_tag="JNC24_003903"
     CDS             complement(1799..2392)
                     /locus_tag="JNC24_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proQ/FINO family protein"
                     /protein_id="HAY9160531.1"
                     /translation="MEKNQEERKTPVIVVKKRRTFSLPSLSEKTDIIAPVFTEQTAES
                     APAGINSSAVETHIPEAPARKKKKKRHRFPRPSHWTREYTHECVEKIKALFPHLRAEG
                     GGFIPLKIGINNDISAFLAESPDTELSMDEWFCAVSCITSRRVYLQRTSVAGVPRYGL
                     DGHPDGQVSETEAQSAVQRLAIIEQRWLRMKERQTEQ"
     gene            complement(2839..4047)
                     /locus_tag="JNC24_003904"
     CDS             complement(2839..4047)
                     /locus_tag="JNC24_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HAY9160532.1"
                     /translation="MTRSGGDFQPRPLKRLFTANQCWTSFLDAGGLRDIEVEAVTKML
                     ACGTRILGVKEYNCDKPECPHVRYVTNSCGSRACPSCGKKATDLWIATQLNRLPDCDW
                     VHLVFTLPDTLWPVFESNRWLLNDVCRLAVENLLYAARKRGQEPGIFCAIHTYGRRLN
                     WHPHVHVSVTCGCLNKHGQWKKLSFLKDAMRSRWMWNMRQRLLKAWSEGLAMPESLSH
                     ITTESQWRSLVLKAGGKYWHVYMSKKTAGGRNTARYLGRYLKKPPIAASRLAHYNGGA
                     SLSFRYLDHKTGETATETLTQRELVARLKQHIPEKFFKMVRYFGFLANRVCGEKLPQV
                     YRALGMDKPEPVAKVCYVQMVKQFLSRDPFECVLCGGRMVYRRAIAGLNVEGLKKNAR
                     DISLLRYMPA"
     gene            complement(4028..4261)
                     /locus_tag="JNC24_003905"
     CDS             complement(4028..4261)
                     /locus_tag="JNC24_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001366937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9160533.1"
                     /translation="MVKEVLKAVARANNHPYQSVFADFITGHPSCTVCFWETFHKMYP
                     DSPYEYVTFCHTCRRFDLYETEAEMKADDPKWW"
BASE COUNT         1024 a         1033 c         1004 g         1204 t
ORIGIN      
        1 cacccccatt ttgttctggt ttatgaggtc gacagccagt ggggaaaagt gtatgtcctg
       61 cgcgtgttgc ataccgcaca gaagtggcca taaaattcca gtcaggaata atacttttca
      121 tttttagttt actgctcccc cagtaagagc gatatattcc gaacagtctt gtctgggagg
      181 tatggtgcag tcagcgaaga accgccgaga atcctcgccc aatgcgggcg gggagcagtc
      241 accggagtct tcgccacccg ccctctgtgc actgaccggg tgatcctgtc agtgagcgtg
      301 gttctgccac ttcatccgaa ttctttcttc cagtttgcta tgcaatccgc ctccgatcca
      361 ctggctgacc agagctgctg ttgcaaatga acagaaagtc aaggcgaatc catgtttaaa
      421 cgtctccggt agttcctggc cggcgtgctg agtgatgatg gaaaacccta tatatataat
      481 aaaggtacaa aaaattcctg tgtgggcgag ccaggcaaac catttggcca ccctgtataa
      541 cccgtatttc aatggcatct cccgtttaat atcatgaata atcattgtat ctatcttgtg
      601 ggtcagagat tgaaattttg taaatatcat aagtccttag tccatctaaa gtgagattta
      661 ccgtatctga atatatcaaa atgctaaggc agaatccccg gaaatttgtg tattacttct
      721 gtagcagaca cggcaaaatc gttgcattac aggtgcatct gctggggcgt ctgtttatcg
      781 atgcatcaga tttaatgtct gacagttact tttctgtatc acccacactc tctgcttttt
      841 cattgctact gtcatattta tgtctgttgc gcggttttat ctgtgtatat aaaatacatc
      901 tgtattcatt gttttctaaa aatttgcgta tgttgataaa aaatccgatt tcttatttcc
      961 atattggcac gtttttattt tcaggaaaaa aacgtcatct tctggaatac tggaaagcag
     1021 aagttatcat gcgggataag tttgtttttc accggcttat caggaacggt atgcagaggc
     1081 agagaggttt tcttctgtgg tggcgcctgg ctaatgaaat gtatatattg gggaataaaa
     1141 aacaacgaaa atgtgcgatc aagataaaaa atacgcttat ggcaaagtat ggatgtgata
     1201 ttggtctggg ggcgcagatt ggtaaaggcc tcgtgctgcc ccaccattca ggcgtcgtca
     1261 ttcacggaaa tgtaaaaatc ggtgagaatg tgattatacg gcagaatacg accattggcg
     1321 aaaaggaatc tgacagcaga gagaattata tcgttattgg tgataatgtc gatatcggtg
     1381 cccatacgtg tattatcggg cttaacgtaa aaataggcag taatgtaaaa attggagcca
     1441 tgtcttttat tatggaggag gttccggata actgtactta cgttaccagg aaagaatcac
     1501 gggtaattat gcgttaatca tgatgatatt attcacagtt ttattgtatt gttgattgag
     1561 aaatttatac gtgcgggggg tatggcctga gtgcaggaag gaagcccccg tgattgtggt
     1621 gatttttttg ttactttatt tcagtatgcg gcagatatcc attgcatcct tgcggtcgct
     1681 tttttccttt caaaaggcag ttacgtcgct tctgccttac atctgatgag cctttggtgg
     1741 catccatgag tctgtaagca gactctgata cacaatgtca tgatgttatc tgaaatgttt
     1801 attgctcagt ctgccgttcc ttcatcctga gccaccgttg ttctattatc gccagtcttt
     1861 gaacagcact ctgtgcttca gtttctgaga cctgcccatc gggatgacca tccagcccat
     1921 aacgagggac gccggcgaca gaggtccgct gcagatatac gcggcgggag gtgatgcagg
     1981 acactgcaca gaaccattcg tccatggaca gttctgtatc cgggctttct gccagaaatg
     2041 ccgaaatatc attgtttatg ccaattttca ggggaataaa tccaccacct tcagcacgaa
     2101 gatgaggaaa cagggccttt attttttcca cgcactcgtg agtatactca cgggtccagt
     2161 gggagggacg tggaaaacgg tgtcttttct ttttcttctt cctggctggc gcttcgggga
     2221 tatgtgtttc gacggcagat gaatttattc cggcgggggc tgattctgcc gtctgttcag
     2281 taaatacagg tgcgataata tctgtttttt cggataagga cggtagcgaa aaagtacgac
     2341 gttttttcac cacaatgacg ggagttttcc tttcttcctg atttttttcc ataaatacct
     2401 cggctggctg gttttactct ttaataatat cacaattcat aaattagtaa attttccatc
     2461 aggtgatttt tcatggctaa taagtccgtt ctctgaatat ttatactcga ggtagtaaca
     2521 gttatggcgt gacagtcgaa ttgatattct actgaaccct gacagaaaca tggttgatat
     2581 attctctcac ttttatgttg tcatgatata aataaaaaca attcatttga aaggatatca
     2641 gaagaagaaa tcggggcgca cagttcacgt ataggaaatt gaaaaactaa gcgatacccc
     2701 tgtccgcgcc ttatgccacg tgagcctttc tgtttttccc caccactccg gtgttttctc
     2761 tctctgcatg gtcgattctt ataccgcaac aggctgtttt ttatacttgc accgattccc
     2821 gggcacactt agcctgtgtc aggccggcat atacctcagc agactgatat cccgcgcgtt
     2881 tttcttcagc ccctccacat tcagccccgc aatggcccgg cggtatacca tccggccgcc
     2941 acacagcacg cattcgaacg ggtcacgact caggaactgt ttcaccattt gtacatagca
     3001 cactttcgcc actggttccg gtttatccat ccccagagca cggtacacct gcggcagctt
     3061 ctctccacac acccggttgg caaggaaccc gaagtacctc accatcttaa aaaacttctc
     3121 cgggatgtgc tgtttcagcc tcgcgaccag ctcacgctgt gtcagcgttt ccgtcgccgt
     3181 ttctcccgtt ttgtggtcca ggtaacggaa gctcaggctc gcccctccgt tgtaatgtgc
     3241 cagccgggag gccgctatcg gcggcttctt cagataacga cccaggtagc gcgccgtatt
     3301 cctcccgccg gccgtttttt tcgacatgta cacatgccag tattttcctc cggctttcag
     3361 caccaggctt ctccactgtg attccgtcgt gatatgtgac aacgactccg gcatcgccag
     3421 cccctctgac cacgctttca gaagccgttg ccgcatattc cacatccacc gcgaacgcat
     3481 cgcgtctttc aggaagctca gctttttcca ctgaccatgc ttattcagac atccacaggt
     3541 tacagacaca tgtacatgcg gatgccagtt gagacgacgg ccatacgtgt ggatggcgca
     3601 gaagataccg ggttcctgcc cccgtttccg ggcggcatac agcagattct ccaccgccag
     3661 acggcacacg tcattcagca gccaccggtt gctttcgaac accggccaca gcgtgtccgg
     3721 cagggtgaag accagatgta cccagtcgca gtcaggaaga cgattcagct gtgtcgctat
     3781 ccacaggtct gtggcctttt ttccgcagga cgggcaggca cggctgccgc atgagttagt
     3841 gacgtatctg acgtgggggc actcaggttt atcgcagttg tattccttca ctcccagtat
     3901 ccgcgtgccg caggccagca tttttgtgac agcctcaacc tcgatatcgc gcagaccgcc
     3961 cgcatccagg aaggacgtcc agcactggtt ggctgtgaac aggcgtttca gcggtcgggg
     4021 ctgaaaatca ccaccacttc gggtcatccg ccttcatctc cgcttctgtt tcgtataaat
     4081 caaaacgacg gcaggtatgg cagaacgtga cgtattcgta cggtgaatcc gggtacattt
     4141 tgtggaaggt ttcccagaaa cagaccgtgc aggagggatg accggtgata aagtctgcaa
     4201 atacggactg ataagggtga ttattggctc tggcgacggc tttcagaacc tctttcacca
     4261 ttctg
//
LOCUS       DADMKF010000234         4246 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.238, whole genome shotgun sequence.
ACCESSION   DADMKF010000234 DADMKF010000000
VERSION     DADMKF010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4246
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.238"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            <1..1358
                     /gene="dcuC"
                     /locus_tag="JNC24_003906"
     CDS             <1..1358
                     /gene="dcuC"
                     /locus_tag="JNC24_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HAY9160534.1"
                     /translation="VVVIVGVARYIIKGYSATGVLFVGGLLLLIISAIMGHKVLPSSQ
                     ASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMVVKLASKPLQY
                     INSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRGAAAAICASPA
                     AIILAPTSGDVVLAAQASEVSLIDFAFKTTLPISIAAIIGMAIAHFFWQRYLDKKEHI
                     SHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVICMLIASILEF
                     LRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQSLISIATSFG
                     SASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAYLTIPMLQASN
                     LGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELMVPGTAAAVTG
                     K"
     gene            complement(1399..2079)
                     /gene="dpiA"
                     /locus_tag="JNC24_003907"
                     /gene_synonym="citB"
     CDS             complement(1399..2079)
                     /gene="dpiA"
                     /locus_tag="JNC24_003907"
                     /gene_synonym="citB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component response regulator DpiA"
                     /protein_id="HAY9160535.1"
                     /translation="MTAPLTLLIVEDETPLAEMHAEYIRHIPGFSQILLAGNLAQARM
                     MIERFKPGLILLDNYLPDGRGINLLHELVQAHYPGDVVFTTAASDMETVSEAVRCGVF
                     DYLIKPIAYERLGQTLTRFRQRKHMLESIDSASQKQIDEMFNAYARGEPKDELPTGID
                     PLTLNAVRKLFKEPGVQHTAETVAQALTISCTTARRYLEYCASRHLIIAEIVHGKVGR
                     PQRIYHSG"
     gene            complement(2048..3706)
                     /gene="dpiB"
                     /locus_tag="JNC24_003908"
     CDS             complement(2048..3706)
                     /gene="dpiB"
                     /locus_tag="JNC24_003908"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase DpiB"
                     /protein_id="HAY9160536.1"
                     /translation="MLQLNENKQFAFFQRLAFPLRIFLLILVFSIFVIAALAQYFTAS
                     FEDYLTLHVRDMAMNQAKIIASNDSVISAVKTRDYKRLATIANKLQRDTDFDYVVIGD
                     RHSIRLYHLNPEKIGYPMQFTKQGALEKGESYFITGKGAMGMAMRAKTPIFDDDGKVI
                     GVVSIGYLVSKIDSWRAEFLLPMAGVFVVLLGILMLLSWFLAAHIRRQMMGMEPKQIA
                     RVVRQQEALFSSVYEGLIAVDPHGYITAINRNARKMLGLSSPGRQWLGKPIAEVVRPA
                     DFFTEQIDEKRQDVVANFNGLSVIANREAIRSGDDLLGAIISFRSKDEISTLNAQLTQ
                     IKQYVESLRTLRHEHHNWMSTLNGLLQMKEYDRVLAMVQGESQAQQQLIDSLREAFAD
                     RQVAGLLFGKVQRARELGLKMIIVPGSQLSQLPPGLDSTEFAAIVGNLLDNAFEASLR
                     SDEGNKIVELFLSDEGDDVVIEVADQGCGVPESLRDKIFEQGVSTRADEPGEHGIGLY
                     LIASYVTRCGGVITLEDNDPCGTLFSIYIPKVKPNDSSINPIDR"
     gene            4086..>4246
                     /locus_tag="JNC24_003909"
     CDS             4086..>4246
                     /locus_tag="JNC24_003909"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308684.2"
                     /note="activates citrate lyase by acetylation of the gamma
                     subunit; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HAY9160537.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGG"
BASE COUNT         1048 a         1079 c          980 g         1139 t
ORIGIN      
        1 gggttgtggt tattgtgggt gtagctcgct acatcattaa agggtattcc gccactggtg
       61 tgttatttgt cggtggcctg ttattgctga ttatcagtgc cattatgggg cacaaagtgt
      121 taccgtccag ccaggcttca acaggctaca gcgccacgga tatcgttgaa tacgttaaaa
      181 tattactaat gagccgcggc ggcgacctcg gcatgatgat tatgatgctg tgtggatttg
      241 ccgcttacat gacccatatc ggcgcgaatg atatggtggt caagctggcg tcaaaaccat
      301 tgcagtatat taactcccct tacctgctga tgattgccgc ctattttgtc gcctgtctga
      361 tgtctctggc cgtctcttcc gcaaccggtc tgggtgtttt gctgatggca accctatttc
      421 cggtgatggt aaacgttggt atcagtcgtg gcgcagctgc tgccatttgt gcctccccgg
      481 cggcgattat tctcgcaccg acttcagggg atgtggtgct ggcggcgcaa gcttccgaag
      541 tgtcgctgat tgacttcgcc ttcaaaacga cgctgcctat ctcaattgct gcaattatcg
      601 gcatggcgat cgcccacttc ttctggcaac gttatctgga taaaaaagag cacatctctc
      661 atgaaatgtt agatgtcagt gaaatcacca ccactgctcc tgcgttttat gccattttgc
      721 cgttcacgcc gatcatcggt gtactgattt ttgacggtaa atggggtccg caattacaca
      781 tcatcactat tctggtgatt tgtatgctga ttgcctccat tctggagttc ctccgcagct
      841 ttaataccca gaaagttttc tctggtctgg aagtggctta tcgcgggatg gcagatgcgt
      901 ttgctaacgt ggtgatgctg ctggttgccg ctggggtatt cgctcagggg cttagcacca
      961 tcggctttat tcaaagtctg atttctatcg ctacctcgtt tggttcggcg agtatcatcc
     1021 tgatgctggt attggtgatt ctgacaatgc tggcggcagt cacgaccggt tcaggcaatg
     1081 cgccgtttta tgcgtttgtt gagatgatcc cgaaactggc gcactcttcc ggcattaacc
     1141 cggcgtattt gactatcccg atgctgcagg cgtcaaacct gggtcgtacc ctatcacccg
     1201 tttctggcgt agtcgttgcg gttgccggga tggcgaaaat ctcaccattt gaagtcgtaa
     1261 aacgcacctc ggtgccggtg cttgttggtt tggtgattgt tatcgttgct acagagctga
     1321 tggtgccagg aacggcagca gcggtcacag gcaagtaaat agtaatgccg gaggcaagtt
     1381 tcctccggct ttatcatgtc acccactgtg gtatatgcgt tgtggtctgc caactttgcc
     1441 gtgaacaatt tcagcaataa tcagatggcg gctggcgcaa tattcaagat aacgcctggc
     1501 agtggtgcag ctgatggtca gtgcctgcgc caccgtttct gccgtatgtt gcacaccagg
     1561 ctctttaaac agttttcgca ccgcgtttag cgtcaagggg tcaatgccgg tcggtagctc
     1621 gtccttaggt tcaccgcgag cataagcatt aaacatctca tcaatttgct tctggctggc
     1681 gctatcaata ctttccagca tatgtttacg ctggcggaaa cgggttagcg tttgccccag
     1741 ccgttcatag gcaatgggct taatgagata atcaaataca ccacaacgta cggcttcaga
     1801 caccgtttcc atatcgctgg ctgcagtggt aaacaccacg tcgccgggat aatgcgcctg
     1861 caccagttca tgcagtaaat taatccctct accgtcagga agatagttat cgagcaagat
     1921 tagccccggc ttaaaacgct cgatcatcat tcgggcctgc gccagatttc ccgccagtaa
     1981 tatctgactg aatccgggaa tgtgacgaat atattccgca tgcatctctg ccagcggcgt
     2041 ttcgtcctca acgatcaata gggttaatgg agctgtcatt aggtttcact ttcggaatat
     2101 agattgaaaa taaggtaccg cagggatcat tatcttcgag agtgataaca ccaccgcagc
     2161 gcgttacgta gctggcaatc aagtacaacc caatgccatg ttcaccgggc tcgtcagcac
     2221 gcgtactgac cccctgctca aatattttgt ctcttagaga ctctggaacg ccgcagccct
     2281 gatcggcgac ttcaatcacc acatcatcgc cttcatcgct gaggaataat tcaacgatct
     2341 tgtttccttc atcgctacgc aggctggctt cgaaggcgtt atcaagtaaa ttgccaacaa
     2401 tggcggcaaa ctcggtgcta tccagtcctg gcggcagttg cgaaagctgg ctaccgggga
     2461 cgatgatcat ttttagcccc agttcgcggg cgcgctgcac tttaccaaaa agcagccccg
     2521 ccacctggcg atcggcaaac gcctcacgca ggctgtcaat aagctgttgc tgggcctgag
     2581 actccccctg caccatcgcc agcacgcgat catactcttt catctgcaac agaccattga
     2641 gcgtcgacat ccaattgtga tgctcgtgtc gcaatgtacg aaggctctcg acgtattgtt
     2701 ttatttgcgt cagttgcgca ttgagggtgg aaatttcgtc tttactacga aagctgataa
     2761 tggcccccag caaatcatca cctgaacgaa tagcttcccg gttggcaata acgctcagac
     2821 cattaaagtt cgccaccaca tcctgacgtt tttcatcaat ctgttcggta aagaaatcgg
     2881 cgggcctgac cacttcagca atgggtttac ccaaccattg ccgtccggga gagctcagcc
     2941 ccagcatctt tcttgcgtta cgattgatgg cggtaatgta accatgcgga tccaccgcaa
     3001 tcagcccttc ataaaccgaa ctaaacagcg cctcttgctg acggaccacg cgtgcgattt
     3061 gctttggctc catgcccatc atttgtcgac ggatatgcgc ggccaggaac cacgacagca
     3121 acatcagaat ccctaacagc acgacaaaca cgcctgccat cggtaataaa aactcagccc
     3181 gccagctatc gattttactc accaggtagc caatcgacac cacgccgatg acttttccat
     3241 cgtcatcaaa gattggcgtt ttggcgcgca tcgccatccc cattgcccct ttcccggtaa
     3301 tgaagtagct ctcccctttc tccagcgcgc cctgtttggt gaactgcata ggataaccaa
     3361 ttttctccgg attaagatgg taaaggcgga tcgagtgccg gtcgccaatc accacataat
     3421 caaaatcggt atctctttgt aatttgttag cgatggtcgc cagccgtttg tagtcacgcg
     3481 ttttcaccgc agagatgaca ctgtcattgg aggcaataat tttcgcctga ttcattgcca
     3541 tgtcgcgtac atgaagcgtt aaatagtcct caaaactggc cgtaaaatat tgcgccaggg
     3601 ctgcaatgac aaatattgag aacaccagaa tcagcaaaaa gatacgcagc ggaaatgcca
     3661 gtctttggaa aaatgcaaac tgtttattct cgttaagctg caacattgtt ttccttgcgg
     3721 gttgtcccga gcctctttga cggatgtaat taaaatgttg aatcaaaata catagatatt
     3781 aattatgatc gtggcaaatt ttattcatta aatcaattaa atcacttaaa cctgcaggtc
     3841 atttcgtaat tacattaatt aaaaaactta aaaccattaa ataaataaaa aaccactaac
     3901 tctatgtgaa ataaatcaaa atttcacgct gcaatactcc ttagaatgta tagcgaaagg
     3961 agaaaaagat atacctcgat cacccccctt tctcccaagt gaaaataaaa ggttatcaac
     4021 ttgcaacatt gaacaacatt cgttgcaaat cgataacaac atgcaccttc aggatactat
     4081 ttattatgtt cggcaatgat attttcaccc gcgtaaaacg ttcagaaaat aaaaaaatgg
     4141 cggaaatcgc ccaattcctg catgaaaatg atttgagcgt tgacaccaca gtcgaagtat
     4201 ttattaccgt aacccgcgat gaaaagctta tcgcgtgcgg tggaat
//
LOCUS       DADMKF010000235         4192 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.239, whole genome shotgun sequence.
ACCESSION   DADMKF010000235 DADMKF010000000
VERSION     DADMKF010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4192
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.239"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(<1..215)
                     /locus_tag="JNC24_003910"
     CDS             complement(<1..215)
                     /locus_tag="JNC24_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAY9160538.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDT"
     gene            complement(330..405)
                     /locus_tag="JNC24_003911"
     tRNA            complement(330..405)
                     /locus_tag="JNC24_003911"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(370..372),aa:Thr,seq:ggt)
     gene            complement(412..486)
                     /locus_tag="JNC24_003912"
     tRNA            complement(412..486)
                     /locus_tag="JNC24_003912"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(451..453),aa:Gly,seq:tcc)
     gene            complement(603..687)
                     /locus_tag="JNC24_003913"
     tRNA            complement(603..687)
                     /locus_tag="JNC24_003913"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(651..653),aa:Tyr,seq:gta)
     gene            complement(696..771)
                     /locus_tag="JNC24_003914"
     tRNA            complement(696..771)
                     /locus_tag="JNC24_003914"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(736..738),aa:Thr,seq:tgt)
     gene            1133..2083
                     /gene="coaA"
                     /locus_tag="JNC24_003915"
                     /gene_synonym="panK"
     CDS             1133..2083
                     /gene="coaA"
                     /locus_tag="JNC24_003915"
                     /gene_synonym="panK"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HAY9160539.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHAVEEVRLRK"
     gene            complement(2112..3077)
                     /gene="birA"
                     /locus_tag="JNC24_003916"
     CDS             complement(2112..3077)
                     /gene="birA"
                     /locus_tag="JNC24_003916"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HAY9160540.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQYLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3074..4102)
                     /gene="murB"
                     /locus_tag="JNC24_003917"
     CDS             complement(3074..4102)
                     /gene="murB"
                     /locus_tag="JNC24_003917"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HAY9160541.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQAGGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1080 a         1042 c          936 g         1134 t
ORIGIN      
        1 gtgtcgtatt caacgtgaga agtgttgatg gtgataccac gagctttttc ttccggcgcg
       61 ttatcgatct ggtcgaatgc gcgagcagca ccgccgtagg ttttagccag tacggtagtg
      121 attgcagcgg tcagcgttgt tttaccatgg tcaacgtggc cgatagtacc gacgttaacg
      181 tgcggttttg tacgttcaaa cttttcttta gacatcgatt gtccctctaa gacacggata
      241 aatcggtgat atcaccacat caaccaggca acatgcccga cttgttgaat gcaataaaca
      301 gaaggaaaaa acagggagga gaaaagaagt ggtgctgata ggcagattcg aactgccgac
      361 ctcaccctta ccaagggtgc gctctaccaa ctgagctata tcagcacatc ttggagcggg
      421 cagcgggaat cgaacccgca tcatcagctt ggaaggctga ggtaatagcc attatacgat
      481 gcccgcatcc tggaactcgg ctacctgatt ttcattctgc actgaatatc gagagaagct
      541 ctctttattc gagccggtaa gcgaacttat cgtctcgggc tacgccatcg cgtggccgaa
      601 attggtggtg ggggaaggat tcgaaccttc gaagtctgtg acggcagatt tacagtctgc
      661 tccctttggc cgctcgggaa ccccaccgga cttgatggtg ccgactaccg gaatcgaact
      721 ggtgacctac tgattacaag tcagttgctc tacctactga gctaagtcgg catcaagtag
      781 cgcgcattct atggagacat gcgagttcat gcaactaaaa aattgcataa tttgttttat
      841 tggtcacatt ttatgcgaca cgatgaagaa acagccgaac ggagggcaga ttagcacact
      901 ttttcaacat cattgtgctc aacaatgcgc tcctgctaaa ccataattct ttttatcaga
      961 tggaatatct gtcacattgc ttttcaacga tagcttcctg gcagagattt tttcttatta
     1021 ttcctcccca tctggtgtta ccctcctgcc cattaaccca ttcaacagaa ctgtgacgcg
     1081 ccatggcaaa tatcgctttg ccgatagagc tatgaccgcc agaaacatgc ttatgagtat
     1141 aaaagagcaa acgttaatga cgccttacct acagtttgac cgcaaccagt gggcagctct
     1201 gcgtgattcc gtacctatga cgttatcgga agatgagatc gcccgtctca aaggtattaa
     1261 tgaagatctc tcgttagaag aagttgccga gatctattta cctttgtcac gtttgctgaa
     1321 cttctatata agctcgaatc tgcgccgtca ggcagttctg gaacagtttc ttggtaccaa
     1381 cgggcaacgc attccttaca ttatcagtat tgctggcagt gtcgcggtgg ggaaaagtac
     1441 aaccgcccgt gtattgcagg cgctattaag ccgttggccg gaacatcgtc gtgttgaact
     1501 gatcactaca gatggcttcc ttcaccctaa tcaggttctg aaagaacgtg gtctgatgaa
     1561 gaagaaaggc ttcccggaat cgtatgatat gcatcgcctg gtgaagtttg tttccgatct
     1621 caagtccggc gtgccaaacg ttacagcccc tgtttactcg catcttattt atgatgtgat
     1681 cccggatgga gataaaactg ttgttcagcc tgatatttta attcttgaag ggttaaatgt
     1741 cttacagagc gggatggatt atccacacga tccacatcat gtatttgttt ctgattttgt
     1801 cgatttttcg atatatgttg atgcaccgga agacttactt cagacgtggt atatcaaccg
     1861 ttttctgaaa ttccgcgaag gggcttttac cgacccggat tcctattttc ataactacgc
     1921 gaaattaact aaagaagaag cgattaagac tgccatgaca ttgtggaaag agatcaactg
     1981 gctgaactta aagcaaaata ttctacctac tcgtgagcgc gccagtttaa tcctgacgaa
     2041 aagtgctaat catgcggtag aagaggtcag actacgcaaa taatttgcag gggagcgaat
     2101 actccccttt cttatttttc tgcactacgc agggatattt caccgcccat ccagggtttt
     2161 attattccat cctgctcaag taataaagcc ccctgtttgt ctattccgcg tgaaatgcca
     2221 aatatttctt tatcaccaat gataagtttc actgggcgat taataaaatt atccagcttt
     2281 tcccagcgcg acagataagg tgccaatcct tcttgttcga agagttccaa cgcagcacgt
     2341 aattcacgta ttagcatggc cgccaacgta ttacgatcga gattgatccc cgcttcctgc
     2401 agcgtgatcc acccctgatt aacgacactc tcttcaacac ggcgcattgc catgttgatc
     2461 ccggctccaa tgactatttg cgccgcatcg ccagttttgc cagtcagctc cacaagaatg
     2521 cctgccagct tgcgatcctg cagatagagg tcattaggcc atttgacacg aactttatct
     2581 gctcccagct tgcgtaatac ttccgccatc acgataccga taaccagact taaaccaatc
     2641 gccgccgccg ggccttgttc cagacgccag aacatcgaca aatataagtt tgcgccaaaa
     2701 ggcgaaaacc atttccgccc ccggcgacca cggccagcct gctggtattc tgcaacacag
     2761 gcatcgcccg atttaagctc tccgatacga tcaagaaggt actgattcgt ggagtcaata
     2821 actggcagca cggctacact accgccatcc agctgaccca atatctgttc agcattaagt
     2881 aactggatgg gctcaggcag gctgtatcct ttacccggaa cggtaaagac atcaacgccc
     2941 cagtcacgca gtgtctgaat gtgtttatta atagccgccc ggctcattcc cagcgtttca
     3001 cccaactgct cgccagagtg aaattcaccg ttcgctaaca gggcaatcaa tttcagtggc
     3061 acggtgttat ccttcatgaa attgtctcca ctgcgctcac ttcacctgat gcaccaataa
     3121 agcggacttc aggctcaagc cagacattaa atttttcacc cactttttgt cttacatggt
     3181 gtgccagttg caccacatct tcgctttttg cattgtcttc attaatgaga actaacgcct
     3241 gttgacggtg caccgcagcc ccacccattt gcatcccttt tagctggcac tgatcaataa
     3301 gccaacctgc tgccagtttt actgaaccac ccgcctgggg gtaatttggt gctgttggaa
     3361 attgcgccag taatgcttta gccgtttcgg cagatacaac agggtttttg aagaaactac
     3421 cggcattgcc attcactttt ggatcaggga gtttggtggt gcgcatatga cacaccgcat
     3481 taaatacttg ctgtggcgtt actgttgtag gatccagacg agttaagtca ccatacgtta
     3541 gtacaggttg ccactctttt ggcagacgca gacctacggc tacaatggcg aagcgatcct
     3601 ggtattcatg tttaaaaata ctgtcgcgat agccaaaacg gcactctttg gcagttaagc
     3661 gcacttgctt gcctgtcgcc agttcaacac aatcaacata agcgcaaact cgctgtaatt
     3721 ctacgccata agcaccaata ttctggatag gtgatgagcc gacacaacca ggaattaatg
     3781 ccagattttc cagaccaggc ataccttcct gcaaagtgta ttttaccagg cgatgccagt
     3841 tttctccggc tcctacatgt aaataccacg catcaggttc atcatgaatt tcgatacctt
     3901 tgatccggtt gatgatcacc gtgccacgat agtcttccag aaaaagtaca ttacttcctt
     3961 cacccagaat aagaacgggt tgtccttctg cggttgcatg ctgccaggca ttgagtaatt
     4021 gttgttcgtc ttcggcacat acaatgtgct gagcattatg atcaatgcca aatgtgttcc
     4081 agggttttaa ggagtggttc atagctgctt tcctgatgca aaaacaaggc tagtttaccg
     4141 tatctgtggg gggatggctt gtagatatga cgacaggaag agtttgtaga aa
//
LOCUS       DADMKF010000236         4185 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.240, whole genome shotgun sequence.
ACCESSION   DADMKF010000236 DADMKF010000000
VERSION     DADMKF010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4185
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.240"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            <1..324
                     /locus_tag="JNC24_003918"
     CDS             <1..324
                     /locus_tag="JNC24_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY9160542.1"
                     /translation="FYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGKATGIYYS
                     AGSIATFTIPLITAHLSQRSIADIMWFDTAIAAIGFLLALFIGLRSRKKTRHHSLKEN
                     VAPGG"
     gene            551..1816
                     /locus_tag="JNC24_003919"
     CDS             551..1816
                     /locus_tag="JNC24_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY9160543.1"
                     /translation="MSQNKAFSTPFILAVLCIYFSYFLHGISVITLAQNMTSLAEKFS
                     TDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPNLT
                     LAYGLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSYML
                     LNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGVSS
                     VLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLKKM
                     VRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEFFP
                     KSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFSRY
                     YRVFIIPENDVRFGERKFSTRLNTIKHRG"
     gene            1828..2694
                     /gene="ydiB"
                     /locus_tag="JNC24_003920"
     CDS             1828..2694
                     /gene="ydiB"
                     /locus_tag="JNC24_003920"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HAY9160544.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTVTDLADQQAFAEVLASADILTNGTKVGMNPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            2725..3483
                     /gene="aroD"
                     /locus_tag="JNC24_003921"
     CDS             2725..3483
                     /gene="aroD"
                     /locus_tag="JNC24_003921"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HAY9160545.1"
                     /translation="MKTVTVKDLVIGAGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLTATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            3627..>4185
                     /locus_tag="JNC24_003922"
     CDS             3627..>4185
                     /locus_tag="JNC24_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9160546.1"
                     /translation="MKPVKPPRINGRVPVLSAQEAVNYIPDEATLCVLGAGGGILEAT
                     TLITALADKYKQTQTPRNLSIISPTGLGDRADRGISPLAQEGLVKWALCGHWGQSPRI
                     SELAEQNKIIAYNYPQGVLTQTLRAAAAHQPGIISDIGIGTFVDPRQQGGKLNEVTKE
                     DLIKLVEFDNKEYLYYKAIAPDIAFI"
BASE COUNT         1092 a          938 c         1004 g         1151 t
ORIGIN      
        1 ttttatgtgg tgataatatt tgcttttgtc attggtttta cctctgctgg aggtgttgtg
       61 caaattggcc tgacgttaat ggctgaacgt ttcccttacg ctaaaggtaa agctacaggg
      121 atctattaca gtgcgggcag tattgcgacc tttactattc cgttgattac ggctcatctg
      181 tcccaaagaa gtattgccga tattatgtgg ttcgataccg ccatcgctgc catcggtttt
      241 ttactggcac tgtttatcgg cttacgcagc cgcaaaaaaa cgcggcatca ctcgctaaag
      301 gaaaatgtcg ctccgggtgg gtaatgcaat attcttttca ggtcatgcaa gatcttacgg
      361 ataaataact ctttctgcgc taactaagga aaatcgcgat caaaaacaaa ctatgacatg
      421 caatattcct ggaaacataa actttatgcc atgtacccag ggaaaatcat cttcagtata
      481 gtaattatgt aaaccgtcgg agaacaatac gtacggtaac gaaattatct ttcagcaagg
      541 agctgtgaaa atgtctcaaa ataaggcttt cagcacgcca tttatcctgg ctgttctttg
      601 tatttacttc agctatttcc tgcacggcat tagtgttatt acgcttgccc aaaatatgac
      661 atctctggca gaaaagtttt ccactgacaa cgcgggcatt gcctatttaa tttccggtat
      721 cggtttgggg cgattgatca gtattttatt cttcggtgtg atctccgata agtttggtcg
      781 tcgggcggtg atattaatgg cagtaataat gtatctgcta ttcttctttg gtattcccgc
      841 ttgcccgaat ttaactctcg cctacggtct ggcagtgtgc gtaggtatcg ctaactcagc
      901 gctggatacg ggtggctacc ccgcgctcat ggaatgcttt ccgaaagcct ctggttcggc
      961 ggtcatactg gttaaagcga tggtgtcatt tgggcaaatg ttctacccaa tgctggtgag
     1021 ctatatgctg ctcaataata tctggtacgg ctatgggctg attattccgg gtattctgtt
     1081 tgtactgatc acgctgatgc tgttgaaaag caaattcccc agccagttgg tggacgccag
     1141 cgtagctaat gaattaccgc aaatgaacag caaaccgtta gtctggctgg aaggtgtttc
     1201 atcggtactg ttcggtgtag ccgcattctc gaccttttat gtgattgtgg tgtggatgcc
     1261 caaatatgcg atggcttttg ctggtatgtc agaagctgag gcattaaaaa ccatctctta
     1321 ttacagtatg ggctcgttgg tctgtgtctt tatttttgcc gcactactga aaaaaatggt
     1381 ccggcccatc tgggctaatg tatttaactc tgcgctggca acaataacag ctgccattat
     1441 ctacctgtac ccttctccac tggtgtgtaa tgccggcgcc tttgttatcg gtttctcagc
     1501 agctggcggc attttacagc tcggtgtttc ggtcatgtca gagtttttcc ccaaaagcaa
     1561 agccaaagtc accagtattt atatgatgat gggtggactg gctaactttg ttattccgct
     1621 gattaccggt tatctgtcga acatcggcct gcaatatatc attgttctcg attttacttt
     1681 cgcgctgctg gccctgatta ccgcaattat tgtttttagc cgctattacc gcgtttttat
     1741 tattcctgag aatgatgtgc ggtttggcga acgcaaattt agcacccggt taaacacaat
     1801 taagcataga ggttaaagga gttaattatg gatgttaccg caaaatacga attgattggg
     1861 ttgatggctt atcctatccg ccacagttta tcgcctgaaa tgcagaataa agccttagaa
     1921 aaagcgggat tgccatttac ctatatggcc ttcgaagtgg ataacgatag ctttcccgga
     1981 gcaattgaag gattaaaagc cctcaaaatg cgcggaactg gtgtgtcgat gccgaacaaa
     2041 caactggcgt gtgaatatgt tgatgaatta acgccagcag ccaaactggt gggtgccatc
     2101 aacaccatcg ttaatgatga tggctatctg cgtggctata acaccgacgg cacgggccat
     2161 attcgcgcca ttaaagagag cggttttgat atcaaaggca aaacgatggt gctgttaggg
     2221 gccggaggtg cctcaacagc gattggcgcg cagggggcaa ttgaaggttt aaaagaaatt
     2281 aaactcttta accgtcggga tgagttcttc gataaagccc tcgccttcgc gcagcgggtt
     2341 aacgaaaaca ccgattgtgt cgtcacggtt accgatctcg ccgatcagca agcctttgct
     2401 gaagtcctgg cttccgccga cattttaacc aatggcacaa aagtaggtat gaaccccctt
     2461 gagaatgaat cattggttaa tgatatcagt ctgttacatc cgggactgct ggtcactgaa
     2521 tgcgtgtata acccgcatat gacgaagtta ttgcagcagg cacaacaagc aggttgcaaa
     2581 acgattgatg gatacggcat gttgttgtgg caaggggctg aacagttcac gttatggact
     2641 ggcaaagatt tccctctgga atatgttaaa caggtcatgg ggttcggtgc ctgacaggct
     2701 gaccgcgtgc agaaagggta aaaaatgaaa accgtaactg taaaagatct cgtcattggt
     2761 gcgggcgcac ctaaaatcat cgtctcgctg atggcgaaag atatcgcccg cgtgaaatcc
     2821 gaagctctcg cctatcgtga agcggacttt gatattctgg aatggcgtgt ggaccacttt
     2881 gccgacctct ccaatgtgga gtctgtcatg gcggcggcaa aaattctccg tgaaaccatg
     2941 ccagaaaaac cgctgctgtt taccttccgc agtgccaaag aaggcggcga gcaggcgatt
     3001 tccaccgagg cttatattgc tctcaatcgt gcagccatcg acagcggcct ggttgatatg
     3061 atcgatctgg agttatttac cggcgatgat caggttaaag aaaccgtcgc ctacgcccac
     3121 gcgcatgatg tgaaagttgt catgtccaac catgacttcc ataaaacgcc ggaagccgaa
     3181 gaaatcattg cccgtctgcg caaaatgcag tccttcgacg ccgatattcc taagattgcg
     3241 ctgatgccgc aaagtaccag cgatgtgctg acgttgctta ccgcgaccct ggagatgcag
     3301 gagcagtatg ccgatcgtcc aatcatcacg atgtcgatgg caaaaactgg cgtaatttct
     3361 cgtctggctg gtgaagtatt tggctcggcg gcaacttttg gtgcggtaaa aaaagcctct
     3421 gcaccagggc aaatctcggt aaatgattta cgcacggtat taactatttt acaccaggca
     3481 taagcaataa tatttcggcg ggaacaccct ccccgccgaa cttaaaaaat atatttaacc
     3541 gtatttaata aaaatatttc gtgagtctct gtgcgctaat tctccatctg gcgtagggaa
     3601 aatcacatct gaatcaggaa ttaacaatga aacctgtaaa accacctcgt attaatggac
     3661 gagtgccggt cctgtcggca caggaagcgg tgaattatat tcccgacgaa gcaacacttt
     3721 gtgtgttagg cgctggcggc ggtattctgg aagccaccac gttaattaca gctcttgctg
     3781 ataaatataa acagactcaa acgccacgta atttatcgat tattagtccg acagggcttg
     3841 gcgatcgcgc cgaccgtggt attagtcctc tggcgcaaga aggtctggtg aaatgggcat
     3901 tatgtggtca ctggggacaa tcgccgcgta tttctgaact cgcagaacaa aataaaatta
     3961 ttgcttataa ctatccacaa ggtgtactta cacaaacctt acgcgccgcc gcagcccacc
     4021 agcctggtat tattagtgat attggcatcg ggacatttgt cgatccacgc cagcaaggcg
     4081 gcaaactgaa tgaagtcacg aaagaagacc tgattaaact ggtcgagttt gataacaaag
     4141 aatatctcta ttacaaagcg attgcgccag atatcgcctt cattc
//
LOCUS       DADMKF010000237         4176 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.241, whole genome shotgun sequence.
ACCESSION   DADMKF010000237 DADMKF010000000
VERSION     DADMKF010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4176
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.241"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            <1..784
                     /locus_tag="JNC24_003923"
     CDS             <1..784
                     /locus_tag="JNC24_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HAY9160547.1"
                     /translation="AKTRHQINLSNSTSFSKDGYSSNNTGITGIAGEHDQLNYGIYVN
                     QQQQNNDTSLGTNLSWRTPIAIIDGSYSHSKNAWQSGGSISSGLVVWPGGINITNQLS
                     DTFAILDAPGLEGAHINGQKYNRTNSKGQVVYDLIIPHRENHLVLDIANSESETELQG
                     NRQIIAPYRGAVSYVQFTTDQRKPWYIQALRPDGSPLTFGYDVLDLQENNIGVVGQGS
                     RLFIRVDEIPTGIKVALNDEQNLFCTITFQHVIDENKTYICQ"
     gene            797..1758
                     /locus_tag="JNC24_003924"
                     /pseudo
     CDS             797..1758
                     /locus_tag="JNC24_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial family protein"
     gene            complement(1796..2695)
                     /gene="nhaR"
                     /locus_tag="JNC24_003925"
     CDS             complement(1796..2695)
                     /gene="nhaR"
                     /locus_tag="JNC24_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HAY9160548.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKSFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAAR"
     gene            complement(2761..3927)
                     /gene="nhaA"
                     /locus_tag="JNC24_003926"
     CDS             complement(2761..3927)
                     /gene="nhaA"
                     /locus_tag="JNC24_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HAY9160549.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMMMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMAVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
BASE COUNT         1249 a          972 c          892 g         1063 t
ORIGIN      
        1 tgccaaaaca cgtcaccaaa ttaacttatc gaattcgacc tcattttcca aagatggcta
       61 ttcctccaac aatactggaa ttactggcat agccggtgaa catgatcagt taaattatgg
      121 tatatatgtt aatcagcaac aacaaaataa tgatacctcg cttggtacga atttaagctg
      181 gagaactccc atcgccataa tagatggcag ctatagtcat tctaaaaacg cctggcaaag
      241 tggtggaagt attagtagtg gattagttgt ctggcccggt ggtattaata tcactaacca
      301 gttatccgat acatttgcaa ttctggatgc gcctgggtta gaaggtgcac atattaatgg
      361 acaaaaatac aatcgaacaa acagcaaagg ccaggttgtt tacgacctga taatacctca
      421 tcgtgaaaac catctggtac ttgatatagc aaacagtgaa agtgaaacag aattgcaggg
      481 caatcgtcaa attattgcac cttaccgtgg agcagtttct tatgtgcagt ttacaactga
      541 ccaacgtaag ccctggtata tacaggcact gcgtcccgat ggttcgccat taacctttgg
      601 ctatgacgta ctggatctcc aggaaaataa tattggagtc gttggccagg gtagtcgcct
      661 ttttattcgc gtagatgaaa ttcccactgg aataaaagtt gctctcaatg atgaacagaa
      721 cttattctgt actattactt ttcaacacgt tatcgatgaa aacaaaacat atatatgcca
      781 gtaaaaggag tttactatga agtggctttt attaatcacc ttgagtctat attcttttat
      841 tgtgcaaagt gctccctgtg cattgaccaa tgtcggggaa caacgtggaa catatatact
      901 caagccactg agtatgaaag gtaatctcac cgccgaaaaa attgaaaaca ccggaaatat
      961 cattgagtgt gcagacttac aagagacgac caacgccatt aaagttcatt ttttaacaca
     1021 gaacgcttta aggtttaaag tcggaagtcg tcattatatt attaatttcc cctttgaatc
     1081 tgacggtaaa aaaaaataga gcttccagga aggtcatctt ataatttaga agaaatgcta
     1141 tctgcgataa atttcaccat tccatactct atcgcatctg tatcagatag ttccaacacc
     1201 gcagatattt ttccaggaac tccttttcct ttgttgttcg aaattgaagt gagttcatgt
     1261 acagggaata atactaattg taatagcgtc cgctttaatt ataatttaaa tactatttta
     1321 caggtaaatt taatgacctg tggttttgaa gatcaatcca tcgacactgg acgatttgta
     1381 ctgtctcgta tcaataatga aaattatcag tttcatcata taagatttga ctgcatccag
     1441 ggcgagcaag gtgaattagt ttttgaaccc agtgatgttg aatattactt tgaaccagtg
     1501 acgatacagg agtcaggaac cagcatatta aaaaatgagt tagaaaacag tgaggacggt
     1561 gcgggtcaag taggctttca accctccaat gatggtacga atgaaatcca gtatggtaaa
     1621 agcaattact actcttttca gcatccccat gaaggaagta atcagattcc tctatttatt
     1681 cgcccgcgta catatggaaa taatgtctca agcgggcaaa taatgagccg agtcaaaatc
     1741 gtggttatgt acaactaatc tatctccccc accttaactt tgggggaggt gccgattaac
     1801 gcgccgctgg actaaaaagc gcagaataat ccgtattgca gattcgctgt accgccgggt
     1861 gctgaatcat ccgctcagca aaaatagcat ggtactcttc catcacattc tcgacgcgac
     1921 caatttctac gacagtttta tcggcataaa agtcatatgc ataaagcgtt ggggcaacga
     1981 agattgcatt gtgcatcgca ccaaaagctt tcatcaacgc ggcatcatca aactcgccga
     2041 ggatttctac gtttaatccc tgggagttaa accagttaag caatttgcgc cctaacattg
     2101 aacgtcgccc aggaatcaaa agtcgccgtt cttccagaca agccgggaac gatttttctg
     2161 gtggtggatt tgtacaccag aaactcacgc cacattcgcc aattctcacg gagaacaggc
     2221 cttcctgctg cgtagagtct atcggacagt cagaaatgat catatccagt ttatgctgac
     2281 ttaattgctc cagcagcatt tcgtgggtgg attcgaagca gcgaagatga atgggctcgc
     2341 cttctaccac tgcggcgtta agtacgctac tgaccaggcg tttggaaagt gcatcagcca
     2401 cgccaacgtc aaacaataaa ttggattctt tgcgatagtt cacaatatcc agcatttcct
     2461 ggcttaaggt gaacatttta tcggcatagc gatagaccag ttctcccagc tcgctgggtt
     2521 cgagaccacg tcccttgcgt ttaaataatt tgccttgcag gcgctcttcc agcgctcgaa
     2581 tctgtccggt aatggtttgt ggagttaaat aaagcgcctc cgctgcgcca accacggaac
     2641 cttctttata gacatgccag aagtaataca agtggttgta attgatatga gacatgctca
     2701 tttctctccc tgataacaat gaaaagggag ccgtttatgg ctccccggta aaccgtcctg
     2761 tcaaactgat ggacgcaaac gaacgcgtaa ccagctgtat ccaattaccg ccgaagagat
     2821 agaaccgacc aggataccga gtttcgccca gttaatcagt tctggatcta cgctaccaaa
     2881 ggccaggctg gcaataaaga tagacatagt aaaaccgata ccgcacagga tccccaccgc
     2941 cataatttgc tgataagtcg ttccctcagg cagatgcgcc agtttcaaac gcagcgccaa
     3001 ccagcagaac agactaatcc ccagcggttt gccaatcagc aagccagcga tgatccccaa
     3061 tggcagaatg gaggtcaagc catccagcgt gacgccttgc agtgaaacgc cagcattagc
     3121 aaatgcaaac agcggtaaaa tcagatacgc cacccacggg tgcaatacat gctccagtcg
     3181 cttcgctgga gaacgcccat gcttctcttt caaaggaata aagaagccga caattacccc
     3241 cgccagggtt gcgtgaaccc ccgatttcaa caccgcagtc cacaacacca cgccaacaag
     3301 aatatagacg cccgtgcggc gtacaccaca cagattcaat accgcgagta ccgcaattgc
     3361 tacagccgcg acgccaagag aggccatcga taagtcatta gtgtagaaca atgcgatgat
     3421 aatgatggcc ccaagatcgt cgataatagc cagagccatc aaaaagatct tcagtgctaa
     3481 cggaacacga cttcccaaca gcgccagcac accaagtgca aaggcaatgt cagtagccgc
     3541 cgggatcgcc cacccttcgc gggtaatcgg atcggcatag ttaaaggcca gatagagtaa
     3601 tgccggaaca atcatcccac caatagcggc gataaccgga aatgcggcct ggcgtaagct
     3661 ggctagcgat ccttgcatca gttcacgttt aacttccaga ccgactaaca ggaaaaatac
     3721 cgccatcagc gcgtcattta tccataacag catgtttttg ttgatttcga gtgaaccaac
     3781 ccggagctga accggcgtct ccagaaagtc gtgataccat ccactggttg cgccgctgtt
     3841 ggccatcatc atcgccagga tagcggcaat gataagaata atgcctcccg aggcatcact
     3901 gctaaagaat cgatgcagat gtttcacttt ttatttctct ttcaggtgaa tagatcgttt
     3961 ttactatttt accccgcaca aatcatcgtt aaaatcagat tatagtgtag gaataactcg
     4021 ttttttacga gcaattaaag aatttgcgcc atcagcggtg agatgagttt ttgacggctg
     4081 taaggcagta tgactttccg tgagacagat gctggccaac gtgggatatt taacgcgggt
     4141 taacatcgtt gtcagagccg catgggtgag ctgttg
//
LOCUS       DADMKF010000238         4130 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.242, whole genome shotgun sequence.
ACCESSION   DADMKF010000238 DADMKF010000000
VERSION     DADMKF010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4130
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.242"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            115..528
                     /locus_tag="JNC24_003927"
     CDS             115..528
                     /locus_tag="JNC24_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HAY9160550.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(573..890)
                     /gene="hspQ"
                     /locus_tag="JNC24_003928"
     CDS             complement(573..890)
                     /gene="hspQ"
                     /locus_tag="JNC24_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HAY9160551.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(948..2138)
                     /gene="rlmI"
                     /locus_tag="JNC24_003929"
                     /pseudo
     CDS             complement(948..2138)
                     /gene="rlmI"
                     /locus_tag="JNC24_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
     gene            2233..2511
                     /gene="yccX"
                     /locus_tag="JNC24_003930"
     CDS             2233..2511
                     /gene="yccX"
                     /locus_tag="JNC24_003930"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HAY9160552.1"
                     /translation="MSKVCIIAWVYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(2508..2837)
                     /gene="tusE"
                     /locus_tag="JNC24_003931"
     CDS             complement(2508..2837)
                     /gene="tusE"
                     /locus_tag="JNC24_003931"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HAY9160553.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(2928..3587)
                     /gene="yccA"
                     /locus_tag="JNC24_003932"
     CDS             complement(2928..3587)
                     /gene="yccA"
                     /locus_tag="JNC24_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HAY9160554.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIVHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(3794..3881)
                     /locus_tag="JNC24_003933"
     tRNA            complement(3794..3881)
                     /locus_tag="JNC24_003933"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3845..3847),aa:Ser,seq:tga)
BASE COUNT         1100 a         1047 c         1017 g          966 t
ORIGIN      
        1 atacttacta acatatagac atatttcccg cttttgctct cattcattcg tattagctgc
       61 atggttggca tgtcggcttt gtcgtacact ttgttaaaca gtctggagga aataatgaaa
      121 gaaaccgata ttgcaggcat tttaacgtcc actcacacca ttgcgctggt gggggcaagc
      181 gacaaacccg atcgtcccag ctatcgcgtg atgaaatatc tactcgacca ggggtatcac
      241 gtcattccgg tttcgccaaa agttgccggc aaaacgctgc tgggccagca ggggtatggc
      301 acgctggcag acgttccgga aaaggtcgat atggtcgatg ttttccgtaa ttcagaagcg
      361 gcgtggggcg tggcacagga agctattgcc attggcgcga aaaccctctg gatgcaactg
      421 ggggtgatta atgagcaagc ggcagtactg gcacgggatg ccgggttgaa tgtggtgatg
      481 gatcgctgcc cggctattga gatccctcgc ctgggcctgg ccaaataaaa aatccccgga
      541 aggcaaaaac cttccgggga ttcgttcagg gatcagttac gcagacgcgg ggcctggagt
      601 tgtttgcgga tggtctgcgc cagctcatcc atggatggct gttcaggatg ttcatcctgc
      661 aattcgctac ttagctgggc ttcagccagg taggtatgaa ccggtaggcc gttatcgtcc
      721 tccatcacta cgtgatacca cggagcggcg cgaagctcgt catttaccgc cagctcatca
      781 ggcgacggtt cagagagcga ataaaccggg tcgatatcca cgaccactcc gagataacct
      841 aacagggaat ggcggacctg ctggccgata ccgaatttgc tggcaatcat agtcacctcc
      901 cgggaaatct ttacctttga tataagggta aaattccaca tttcaagtta catgacgcga
      961 caggcaaacc ctttcagata tagcccttcc ggataggtag cgatcaccgg atgatcggct
     1021 gcctgacgga actgctctat aaattgtaca tcacggccgg catcaattgc ggcatccgcg
     1081 atgattttct gaaataaatc gctggtcatc agaccggaac aggagaaagt caggagaata
     1141 ccgccttcat tcagcaactg aatcgccagc atgttgatat ctttatagcc acgacacgcg
     1201 cccatcaact ggcttttatt ctcaacaaac ttcggcgggt ccatcacgat aacgtcaaat
     1261 ttttcaccgc gatcgcgata agtacgcagc aatttaaaga catcatcacg gacaaactca
     1321 gccttgctca gatccagttt gttcagctca acgttctgcc gtgcaatatc cagcgcttcc
     1381 taggaggtat caacgctgac aacctggctg caaccgccca tcagtgccga tacggcgaaa
     1441 ccaccggtat aggagaaaca gttcagcaca cgtttatttt caacgtagcg gcgggtagcc
     1501 aggcggctgt ctcgctggtc caggtagtag cccgttttgt gtccgtgctg aatatccacc
     1561 agcagcttca tgccgtgttc ttcaatcggc agcagggcag gcggcaactc gccggtgacg
     1621 aggccctggg tcagctccat cccttctttt ttacgtaccg caacatcgct gcgatcgtaa
     1681 atcgcacatt ccgggtacag cgtttgcagg gcactaatta atgccgcgcg ctgatattct
     1741 gccccagcac tcagcagttg cagcaccaga aaattaccga aacgatcgat agtaataccc
     1801 ggcaggccat cagattctcc ggcgattaaa cgatagctgt cgaggccatc tttttgcgcc
     1861 agtcagtcac gccatttttg tgcttgttgc aaacggcggg aaaaaaaagc aatgtcgata
     1921 gactcagacg ggtcaaacgt ccagacgcgc gcccggattt gcgaagctgg cgaataagcg
     1981 ccgcgtgcta accattttcc ctgatgatca acaatatcga tggtttcacc gaggctggct
     2041 ttaccttcca tgcgggcaac ggccccggaa aagacccacg gatggcgacg aagtaatgat
     2101 ttttcgcgcc ctttggctaa cactaaacgt acactcataa tttacttttc tgtcgatgca
     2161 aagaaatggg cgtcattgtc cggagttttc catggatttg caacgcgtcc attaaggata
     2221 aggagagcag caatgtcgaa agtctgcata attgcctggg tttacgggcg agttcagggc
     2281 gtaggatttc gctacaccac acagtacgaa gcgaaaagac tgggtttaac cgggtacgcc
     2341 aaaaatcttg acgatggcag cgtggaagtg gttgcctgcg gtgaagaagg gcaggtggaa
     2401 aaattaatgc agtggttaaa gagtggcggc ccgcgttctg cgcgtgtaga acgggtgctc
     2461 agcgagccgc atcatccctc gggggaatta accgattttc gtatccgtta aatacatttt
     2521 accggttttg gcaggccagc aattttggtg gcttgcttag ccggaccttt cggaaacagt
     2581 cggtataaat agcggctgtt acctttttct tcgccaaatt tattcgccat cgcttttacc
     2641 agcatacgaa tcgccggaga agtattgaat tccagataga aatcacgcac aaaacgcacc
     2701 acttcccagt gttccggtga cagcgaaatc ccttcgttct ctgcaatcac cactgccagc
     2761 ggctcactcc actggctgct ttctttgaga tagccttcgg tatccgtttc tatctcttta
     2821 ccttcgaaga tcagcataat tattactacc ttaatcagac tggcggcagt gtaacaaaaa
     2881 acaaagcccc gcataaagcg aggctatgaa agtgttagcg ggtgagatta atcgcggctg
     2941 gcgaagccca gaatgctcag caggctgacg aagatgttgt acagcgaaac atacaggcta
     3001 accgtggcac gaatatagtt cgtttcaccg ccatgaacga tgttgctggt ttcaaacaaa
     3061 atagcgccag aggagatcag aatgaagacc gcgctgatcg ccagatgcag agcaggcagc
     3121 tgcaggaaga tattagcaac cataccaatc agcaccacca caatacccgc catcagcata
     3181 ccgccgagga acgacatatc tttgcgggtg gtcagcacat atgcagagca gcagaagaac
     3241 actaacgccg ttccgcccag tgccatagcg attacgtcac ccattccggc agacagatag
     3301 gtgttcagaa taggtccgag gatataaccc agaaaaccgg taaaggcgaa tgcggagata
     3361 atcccggtcg gcttattcgc cgttttatag gtcaggaaca tcaaaccata catacccacc
     3421 agcgtcagaa tcagacccgg agatggcagc atcagcaccg tactggcagt tgcggtaatc
     3481 gccgaaaagg ccagcgtcag gctcagcaga aaataggtat tacgcagcac cttatgggtg
     3541 ctaagcagtg atgtacggtc atgtgaagaa ctaacaatac gatccattga gtcactctct
     3601 atgacagatg taattaatta agcagcataa tgataatgcg taagggcacc cagaagtttt
     3661 acccatcttt acgcatttga tctggaacag gtttaacagc ggattatcag gtcattaagc
     3721 aaatataacg ccctgagaat ttcgacaggc aaaagaaaaa ggggttagca tttagctaac
     3781 cccttatctt atttggcgga agcgcagaga ttcgaactct ggaacccttt cgggtcgccg
     3841 gttttcaaga ccggtgcctt caaccgctcg gccacacttc cggaatgacg cgcactataa
     3901 acatcccgat gcggcgtgta aacccctaat ttgtttgttt gcctgaaaaa cagccaaaag
     3961 tgcattgata gcgtgaaata acagcagatt gatcatttca tcaccatgaa ttccttctct
     4021 tttactcgtt tagcaaccgg ctaaacatcc ccaccgcccg gcaaaaagaa aaataggtcc
     4081 atttttatcg ctaaaagata aatccacaca gtttgtattg ttttgtgcaa
//
LOCUS       DADMKF010000239         4125 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.243, whole genome shotgun sequence.
ACCESSION   DADMKF010000239 DADMKF010000000
VERSION     DADMKF010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4125
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.243"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            <1..83
                     /locus_tag="JNC24_003934"
     CDS             <1..83
                     /locus_tag="JNC24_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016504975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HAY9160555.1"
                     /translation="FWHDLAALVIAGILASMIVNWLNKRK"
     gene            459..566
                     /locus_tag="JNC24_003935"
     CDS             459..566
                     /locus_tag="JNC24_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HAY9160556.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(653..2332)
                     /gene="bcsG"
                     /locus_tag="JNC24_003936"
     CDS             complement(653..2332)
                     /gene="bcsG"
                     /locus_tag="JNC24_003936"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HAY9160557.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFATFLLMPIPRYSLHRLRHWIALPIGFALFWHDTWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGASVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(2329..2520)
                     /gene="bcsF"
                     /locus_tag="JNC24_003937"
     CDS             complement(2329..2520)
                     /gene="bcsF"
                     /locus_tag="JNC24_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HAY9160558.1"
                     /translation="MMTISDIIEIIVVCALIFFPLGYLARHFLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(2517..4088)
                     /gene="bcsE"
                     /locus_tag="JNC24_003938"
     CDS             complement(2517..4088)
                     /gene="bcsE"
                     /locus_tag="JNC24_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsE"
                     /protein_id="HAY9160559.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLARDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
BASE COUNT         1051 a         1030 c         1074 g          970 t
ORIGIN      
        1 ccttctggca tgatttagcg gctctggtca ttgctggcat tcttgccagt atgatcgtga
       61 actggctgaa caagcggaag taacgtgtca tgcgggcgtc aggctgccgt aatggcaatt
      121 tgcgcccgga ccaggccgca ggggtgaaac tctgcggcct ttttcgtaag aaaaatgcaa
      181 cgattgcacg cggataatat tctgcggaat gcgcagcaca gagtgaagac gtgacggatt
      241 ccggaacaga aaaacagtcg tagaatcagc ggcaagccgg aaaggttccg gtgaggcgca
      301 atgttgcggg ggctttatcc ctggtggcat tggttgctgg agagagaaaa cccccgcacg
      361 ttgcaggtat gcacctgaca acatcacggg ggctaatctt gaacccaaac aactcaagaa
      421 tagccgcgaa acgttggcat tacaacacag gcggctatat gacgctcgca gaactgggca
      481 tggccttctg gcatgattta gcggctccgg tcattgctgg cattcttgcc agtatgatcg
      541 tgaactggct gaataagcgg aagtaacgtg tcatgcgggc gtcaggctgc cgtaatggca
      601 atttgcgccc ggatcaggcc gcagaggtta aactctgcgg cctttttcgt gcttactgcg
      661 ggtaaggcac ccagtcgccg ccgttcaggc gaacgtacgg tttatcctgg tattgaataa
      721 ctactgcatt tgagttctcg gagaccggtg ctgtttgtgg caacccactg gtgagttttt
      781 tccagtcaac attgtcttcg gtgaaaatct tgccatcaag aacgcgaacc accagatcgg
      841 agatagctag gaagctgctc ggttggtcaa tgacaatcgg tgccccctga tgtggtgcct
      901 tcatgccgaa gaatttcacc ccaacgggga cgtctgtgat agacgggcta gggatatcac
      961 gtaggccaga tacctgcatt ctgtcgccct tcagcgcgcc gccgtgttcc ggcaccacga
     1021 ccaccatcac tttacgaccc gatttctcca gttcagtaaa gaaggcgtcc agttcatcaa
     1081 agaatttctg cgcccgcgct ttgtaatccg ctgttttgct gacccccgga taatggttgc
     1141 cgtcatgcag tggaagcgtg ttgtagaacg tggcactacg gctgttttta tctttttcgg
     1201 taacgtccag ccagcggtta agcacggcgg tatcgtcata aaccggcgaa ccatcaaagc
     1261 ccagcaaaat aaccggcaga tttgtttgat ccatcaattc agtctgcatg ccgccatttt
     1321 cgcgaacttc tttcaaaaaa ccgccgaact ggccgttatg tcccatcatc aggtgctggg
     1381 taaagcccag tttcgaaagg ttatcaaaca gatagcagtc gttatttgcc ggttgataca
     1441 gattagtgtg cgaagtctgc ccgcagctgg cgcgcagtaa acggatcgcc gccgggccac
     1501 tgtaggaggt ggcggagtta aagttcttga actcaatatc gaaatgcgac cacagtggat
     1561 gcgacatcaa cccggcggct tctatatccg accaggaaag cgaacagatg ttaatcacca
     1621 gtagttcaaa tggctgagca tcagcgggca gcgaagacgg gaaggtcgat ttacgtttcg
     1681 cctccgcgtt atagaaatta ttcagccagg cgttaaggtt cgccgttgtt ggcggtgcag
     1741 tttgtgcggg catatcaccc actaccgatg cgccccccgt cgccgcaacg gttgccgctg
     1801 cgttaccacc cgtcgttgtt acagtggtcg tcggttgtcc ggctggccac aaggagaaac
     1861 ttggtcccgc cagggtaagt acgttcagcc atagcagtat ggcaaccaca aaaacggtaa
     1921 tgcgaatcca ttgtgacagg aataaccagg ccactaataa aacaaaaatg gccccaatca
     1981 tctgccagtt aataaagcgt gtgacaaggt cgattaaata atcggtactg aaccccgcca
     2041 cctgcgaacc ctggctcatt atgctttccg ggccaggcaa ccaggtgtca tgccagaaca
     2101 aagcaaagcc gatcggcagg gcaatccagt ggcgcaagcg atgcaggctg tagcgcggaa
     2161 tgggcatcag cagaaacgtg gcaaacacca aattgaggag cggatggaag ttaagatatc
     2221 ccgcccacaa caggccgaac ttaaccagaa aatagaagtt ccagccggaa aggccgcgcc
     2281 agtattgcca gagggaagaa ggcatggcgg tattttgcgt aaattgagtc attttttggt
     2341 tgccctggct ttttccgtgc ggcgtaacgt cccggccggt ttaacataac gaggtttagc
     2401 aaagaataaa cgtaaggtgt cgcgaatgcg tcgcaaaaag tgccgcgcca gatagcccag
     2461 cgggaaaaat atcagtgcgc aaacgacaat aatttcaatg atatcgctga tggtcatcat
     2521 gatgagcgct ccacagcatc atctaacagt cgcatgggtt ctggtattcg tcgccagtgg
     2581 cgaccatcgt gctcggcatt gatgaccggt ttagaacttt gcgttaaagg cagcggcatg
     2641 ccccattgtt ctggggcaag caagcgcatc tgcaccagct cggcactgat ttgatcatct
     2701 tcaaaccaga ccatacggtt tgagaaaatg tcgccagtag gcaatgggaa aatatgattc
     2761 aacgcggtat ccagatcgtt aatccgacag aatgagagaa acagcaccag ccgattaccg
     2821 ccaatggtca tgatatcgcc ggtacggtta gggcgacaca gcgtcagggc ttgttcaacg
     2881 cggatacccg gtaccggacg tagggcaacc agaacgcctt taccgtgggc aggtaataga
     2941 gggttattca tcatgttgtt gacggcatta cagaacacat cccacttctg gaaaccacgc
     3001 agtttgagcg gctgggtcat tgacagcaag gtagtgatat cttccggcac atagcgacta
     3061 aacttctgcc cttgcacgct ttcgatcatc gtcagacaac gggagagtgg cgcattccac
     3121 ggaataacca tatttgcacc gcaggccaat aacaaacgtt catcggtggc gcgcaggcta
     3181 gcggtatttt cccgcacgag gattttcatc gcactaccgc gctggcgacg cagggtatga
     3241 atgctgcggg ccagtggctc gatttgcgca ttttgctgta aagaaaagac caccgtcgcc
     3301 gcctgagcgg tacgggcttc attgaacagg acttcgttat tgttgaacag ttgccagtgt
     3361 tccgatagcg gcggcgcacc ttccagtaca gcaacattac tcagaatgcg tttttcgtcg
     3421 ctgcgtggtt ggatctccgc ctcttcgctt tgaactaatg tccagatacc attttgttgc
     3481 tgaacgctaa gctgctgacg ggcgctgacc cctttttcgt tgcaccagaa ggcaatatcc
     3541 agcaaatgtt ggtcaccctg aaaacgcaaa ctggcaagac caaaaagtga acggtattcc
     3601 tcaagcaaca atgaaaattg tttatcgtta ttatttccgg gattaattac caaaagcgaa
     3661 caatggttta acctgctcca tttattcatt ttatccaacc atgagcgaag ccgctcggca
     3721 ggaatgtttt gccatgcgtt atttgcgcaa acaagaataa aaaggtaatt atggggatca
     3781 atagaacact gcaaatcacg ggccaaatag tatagacctt tttcatgatt tagcattgaa
     3841 aataatttta ttttttccgg cccttgagaa tcatctaatt gaaagatttt cgccggatcg
     3901 ctgtccatgc taatgaccgc gacgtgtgcg gtttcagcct gggatgcaat tgtttgattc
     3961 gccagactga tagcatcttc atggcgatcg acgttaaacc accagacgcc gcctgctggc
     4021 atatgtcgca gctcatccca taatgatgag ataccgatag agaatacagg gtccacaatg
     4081 tccctcatgt caccattttg tctgattatc agtttactag cgaaa
//
LOCUS       DADMKF010000240         4089 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.244, whole genome shotgun sequence.
ACCESSION   DADMKF010000240 DADMKF010000000
VERSION     DADMKF010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4089
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.244"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            <1..879
                     /locus_tag="JNC24_003939"
     CDS             <1..879
                     /locus_tag="JNC24_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAY9160560.1"
                     /translation="IGLFIALMGLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFII
                     TVLSSRHFHAAVLVSIVVTSCCGLFFGDVHFSGVYSIPPDISGVIGEVDLSGALTLEL
                     AGIIFSFMLINLFDSSGTLIGVTDKAGLIDGNGKFPNMNKALYVDSVSSVAGAFIGTS
                     SVTAYIESTSGVAVGGRTGLTAVVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLM
                     TSSLARVNWDDFTESVPAFITTVMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLC
                     VVVVASLFALKIILVD"
     gene            932..1384
                     /locus_tag="JNC24_003940"
     CDS             932..1384
                     /locus_tag="JNC24_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HAY9160561.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDGSEQNLLQWHRILQKAR
                     LSAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            1595..2775
                     /gene="nepI"
                     /locus_tag="JNC24_003941"
                     /pseudo
     CDS             1595..2775
                     /gene="nepI"
                     /locus_tag="JNC24_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
     gene            complement(2816..3109)
                     /locus_tag="JNC24_003942"
     CDS             complement(2816..3109)
                     /locus_tag="JNC24_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9160562.1"
                     /translation="MKPTTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKI
                     CTPQASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            3331..>4089
                     /gene="nlpA"
                     /locus_tag="JNC24_003943"
     CDS             3331..>4089
                     /gene="nlpA"
                     /locus_tag="JNC24_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpA"
                     /protein_id="HAY9160563.1"
                     /translation="MKLTTHHLRTGAALLLAGILLAGCDQSSSDAKHIKVGVINGAEQ
                     DVAEVAKKVAKEKYGLDVELVGFSGSLLPNDATNHGELDANVFQHRPFLEQDNQAHGY
                     KLVALGNTFVFPMAGYSKKIKTVAQIKEGATVAIPNDPTNLGRALLLLQKEKLITLKE
                     GKGLLPTALDITDNPRHLQIMELEGAQLPRVLDDPKVDVAIISTTYIQQTGLSPVHDS
                     VFIEDKNSPYVNILVAREDNKNAENVKEFLQSYQS"
BASE COUNT          901 a          907 c         1076 g         1205 t
ORIGIN      
        1 attggattat ttatcgcctt aatgggatta aaaaatactg gcgttattgt cgccaataaa
       61 gacacgctgg tgatgattgg cgatttaagt tctcacggcg tgttgttagg tattttaggg
      121 ttttttatta taaccgtgtt gtcatcacgt cattttcatg ccgcggtgct ggtttctatt
      181 gtggtgacgt cttgctgtgg attatttttc ggtgatgttc attttagcgg cgtctattcc
      241 attccgcctg atattagcgg cgtcattggt gaagtagatt tgagcggcgc gttaacactt
      301 gaactcgccg gtatcatttt ctcctttatg ctgatcaacc tatttgattc atcaggaaca
      361 ttaattggtg taactgataa agcgggctta atagatggta acggtaaatt ccccaatatg
      421 aataaggcgc tgtatgttga tagcgtcagt tcggtggcgg gtgcgtttat cggcacctcg
      481 tctgttactg cctatattga aagtacttct ggtgtggcag tcggtggccg cacggggctg
      541 actgcggttg tggttggcgt tatgttcctg ttggttatgt tcttctcccc gctggtggcg
      601 atggttcctc cttacgcaac cgccggagcg ttaatctttg ttggcgtgct gatgacttcg
      661 agcctggcgc gcgttaactg ggatgatttt accgaatcgg tgcctgcgtt tattaccacg
      721 gtgatgatgc cctttacttt ctcgatcacc gaagggattg cactcggctt tatgtcgtac
      781 tgcatcatga aagtgtgcac cgggcgctgg cgcgatctga acctgtgtgt ggtggtggtc
      841 gcatctctgt ttgcactgaa gattattctg gtggattagt aactggcgag ggtttccaga
      901 tatcatgagt tctgataacg caggagaact catgatctgg ataatgctcg ccacgctggc
      961 ggtagtgttt gtggttggtt ttcgggtgct gacatccggg gccagaaaag cgattcgccg
     1021 tctcagtgat cggctgaata tcgatgtcgt acccgtggag tcgatggtcg atcaaatggg
     1081 aaagtcagcc ggtgacgaat ttttacgtta tttgcatcgc ccggatgagt cgcacctgca
     1141 aaacgccgcg caggtgttgc tcatctggca aattgtcatt gtcgatggta gcgaacagaa
     1201 cctgctgcaa tggcatcgga ttttacaaaa agcccgcctt tccgcgccga ttaccgatgc
     1261 tcaggtcagg ctggcgctgg gttttctgcg cgaaaccgaa cctgaaatgc aggatattaa
     1321 tgcttttcag atgcgctata acgcgttctt tcagcctgcc gagggcgttc actggctgca
     1381 ttaaaaccgc ttcgtcttat cagcctacag accgcgcctg acctgtcggg tggataagac
     1441 gttcacgccg catccggtaa tctttgccct gttccgtttc gatcttaact ccagctcaag
     1501 caacaccgta aaagttgcaa aaacgttaaa tgcgtcacac atttcaatgt taaagtggtc
     1561 ggcttttccc ctgaaacatg ccacgggtaa caccatgagt gaatttattg ccgaaaaccg
     1621 cggcgcggat gccatcaccc gaccgaactg gtcagccgtt ttctcggtgg cgttttgtgt
     1681 cgcctgtctg attatcgttg agtttttgcc cgtcagtttg ttgacgccaa tggcccagga
     1741 tttaggcatt tcggaagggg ttgccgggca atcggtgacc gtgaccgcct ttgtggcaat
     1801 gtttgccagt ttgtttatta cccagacaat tcaggctact gaccgccgca acgttgttat
     1861 tttgtttgcc gttttgctga cgctctcctg cttgctggtt tcctttgcta actcattcag
     1921 tttgctttta atcggtcgtg cctgtctggg gctggcgctg ggcgggttct gggcgatgtc
     1981 ggcgtcgctg accatgcgtc tggtgccgcc gcgtacggtg ccgaaggcgc tgtcggtgat
     2041 cttcggcgcg gtttctattg cgctggtgat tgccgcgccg ttgggcagtt ttttaggcga
     2101 gcttatcggt tggcgcaatg tctttaatgc ggcggcggtg atgggcgtgc tgtgtatttt
     2161 ctggattatc aaatcattgc cttcactgcc aggcgaaccc tcgcatcaga aacaaaatac
     2221 tttccgctta ttacaacgtc cgggtgtgat ggcagggatg atcgccatct tcatgtcttt
     2281 cgccgggcag tttgctttct tcacatatat tcgcccggtg tatatgaacc tggcgggatt
     2341 cagcgtggat ggcttaacgc tggtgctgtt gagttttggc attgccagct ttatcggtac
     2401 gtcgctttcg tcattcattc ttaaacgttc ggtaaaactg gccttagcag gcgcgccgtt
     2461 aatactggct gtgagtgcgt tggtgctgac gttgtgggga agcgataaaa tcgttgctac
     2521 cggcgtggcg attatctggg ggctaacttt tgcattggtt cccgtcggct ggtcaacgtg
     2581 gatcacccgc tcactggccg atcaggcaga aaaagccggg tctattcagg tggcggttat
     2641 tcagcttgtg gcgcggcaat cggcggttat gcgctggata atattggtct gacttcgccg
     2701 ttgatgtttt ctggcacatt gatgttgctg actgcattgt tggttactgc aaaggtgaaa
     2761 atgaagaaat cctgatatca ggtaaagaag cgttggtcag tagttgttgc tgttcttaca
     2821 tatccgggca ttctacctta cccaacgctt cccaataatt gctctgatta ttacgttcca
     2881 tcgccacgat ggcctcgcgt atatgttgtt tattgttttc atctgacaac attaactttt
     2941 cccacgcttc atctgataat gacgcctgtg gcgtgcaaat tttacgtaaa tcttgtaata
     3001 ccgtggtttt ctcttttgcg gactgcagtg agctaaaagg cgactcggcg tatacgatgc
     3061 ctggcatagc gaaaaaagtg aaaataagca gtagtgtcgt tggtttcatg gattcaacct
     3121 tttacaaatg tgtatcgaat gaagcataag tgtagtttgc tttcatcgct ctcatcatgc
     3181 tgtacgaaac gcgcttgcgt ggtcagtaag aagtgccaga ctttatattc cacttttatt
     3241 cctttttatt cttatcgata gcgtttcgtt ttttaaaccg cagcgacctt accgctatag
     3301 tcaggtaatc attaataaaa ggataaaaaa atgaaactga caacacatca tctacggaca
     3361 ggggccgcat tattgctggc cggaattctg ctggcaggtt gcgaccagag tagcagcgat
     3421 gcaaaacaca ttaaagttgg cgttataaat ggcgcagaac aagatgtcgc ggaagtcgct
     3481 aaaaaggtgg caaaagagaa atatggcctc gatgttgaac tggtaggatt tagcggttcg
     3541 ttgctaccga acgatgcgac aaatcatggc gaacttgatg cgaatgtctt ccagcatcga
     3601 ccctttcttg aacaagataa tcaggcgcat ggttataaac tggtagcgct gggaaatacg
     3661 tttgtcttcc ctatggcggg ttattccaaa aaaatcaaaa ccgtggcgca aataaaagaa
     3721 ggcgcgacag tggcgatccc caacgacccg accaaccttg gtcgcgcact tttactgttg
     3781 caaaaagaga agctaattac tctgaaagag ggaaaagggt tattacctac cgcgctggat
     3841 ataaccgata atcctcgtca tttgcagatt atggaactcg agggggcgca gctcccgcgc
     3901 gtactggatg atcccaaagt tgatgtagcg attatcagca ccacttacat tcagcagaca
     3961 gggctttctc cggtgcacga cagcgtattt attgaagata agaattcgcc gtatgtgaat
     4021 attttggtgg cacgggaaga taataagaat gcagaaaacg tgaaggaatt tctgcaatct
     4081 tatcaatca
//
LOCUS       DADMKF010000241         4059 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.245, whole genome shotgun sequence.
ACCESSION   DADMKF010000241 DADMKF010000000
VERSION     DADMKF010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4059
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.245"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            35..1181
                     /locus_tag="JNC24_003944"
                     /pseudo
     CDS             35..1181
                     /locus_tag="JNC24_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000535347.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1475..2716
                     /gene="agp"
                     /locus_tag="JNC24_003945"
     CDS             1475..2716
                     /gene="agp"
                     /locus_tag="JNC24_003945"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HAY9160564.1"
                     /translation="MNKTLIAATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGMVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPVITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRTSAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
     gene            complement(2754..2981)
                     /locus_tag="JNC24_003946"
     CDS             complement(2754..2981)
                     /locus_tag="JNC24_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9160565.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKADKDSLFWGEQTIERKNV"
     gene            complement(3002..3607)
                     /gene="wrbA"
                     /locus_tag="JNC24_003947"
     CDS             complement(3002..3607)
                     /gene="wrbA"
                     /locus_tag="JNC24_003947"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000111568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HAY9160566.1"
                     /translation="MSVKARILVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPE
                     TMPPQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWA
                     SGALYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGG
                     TPYGATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            3659..>4059
                     /locus_tag="JNC24_003948"
     CDS             3659..>4059
                     /locus_tag="JNC24_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAY9160567.1"
                     /translation="MKLLVTYIRWSTKEQDSGDSLRRQTNLIDAFYSKHKNDYYLLPA
                     HRYVDKGKSGFHQQHKNQGSDFRRMFENVMSGAIPEGSLIVVENFDRFSRADIDTAID
                     DVRQILRKGVSILTLGDGELYDKSALTDPVK"
BASE COUNT         1145 a          865 c          954 g         1095 t
ORIGIN      
        1 cgtcattatt tattaaattt tatagacgct atatatgggt agtaatatac atggaattag
       61 ttgcactgca aataattatt tgaaacaggc ctggaacgat ataaaaaatg agtacgaaaa
      121 aaatcaaaca tattcaatca cgctttttga aaacacactg gtgtgtttta tgcggttata
      181 caatgaactc agacgtaaag taaatgaaga ggatactcca tgtctggaat gtgaatcact
      241 agaaaaagaa tttgaggaaa tgcagaatga taatgatcta tcattattta tgagaacatt
      301 gcgtactaat gatacacaaa tttattcagg ggtttcagga ggtattacat atactataca
      361 atatgttcaa aatgttgata ttgttagagt gtccttgccg ggcagaggtt cagagtctat
      421 cacagatttt aaaggttatt attggtatgg ctttatggaa tatattgaaa acattactgc
      481 gtgtgatgat attttttctg agtattgttt agatgatgaa aatatgagta tccagccaga
      541 acagataaat acgccgggaa tatctgattt agatactggt tttaaaagaa catgagtatt
      601 catgggcgct gggcggccat aagacgcaaa agcaagtata cgaagagttt attaaaattg
      661 ttgataaaca aacgatcgag gctcttgttg atacggcatt ctataatctc agggaagatg
      721 taaagacgtt atttggcgtt aatctacaat ctgacaacaa aattcagggg cagggtggtt
      781 ttttatcatg gagttttttg tcttttaaaa aagaatttat tgatagttgc ttgaatgaca
      841 aaaaatgcat actgcattta cccgaattta tatttaatga taacaaggca cgccttgttt
      901 tagacaccga cccagagcag aaggttaatg aggtgaaaaa tttcctcact gctctttcag
      961 atagcatttg ctcattattt attgtttaca gcaatgtggc atcaatctcc ttagggaatg
     1021 aatccttttc aacagatgac gaccttgagt atgggtattt aataaacact ggcaatcatt
     1081 atgacgttta cctccctcct gaactttttg ctcaggctta cgagttaaac aataaggaaa
     1141 ggaatgcgca aattgatttt ttaactcgtt atgcaattta atgacaaagg catgtgctaa
     1201 aaaccattgt tattagcctc acttctctta ttattggtac tattgcctct gtattagtaa
     1261 cgccgctgat attctgttta tccacaggtg caattatcag cggcgtacgc gaggcagggg
     1321 ctaatcaggc atagtttgcg tcaaaccttg cctgtttttg aagatgtata tagaaaaaca
     1381 ggcgttcaac aagccatttt gcgaacctgt tcccggaaaa aagtcatatt tctgtcacac
     1441 tctttaatga ttgataacaa aagaggtgcc aggaatgaac aaaacgctaa tcgccgcaac
     1501 tgtggcaggg atagttttac tcgcttcaaa tgctcaggca caaaccgtac cggaaggcta
     1561 tcagctacag caagtgctca tgatgagccg ccataactta cgtgcgccgc tggcgaacaa
     1621 tggcagtgtg ctggagcagt cgacgccgaa taaatggcca gaatgggacg tccccggtgg
     1681 gcaactcacc accaaaggtg gcgtgctcga agtgtatatg ggccattaca tgcgtgaatg
     1741 gctggcagag caggggatgg tgaaatcggg ggaatgcccg ccgccggaca ccgtttatgc
     1801 ctatgccaat agtctgcaac gtaccgttgc gaccgcacag ttctttatta ccggcgcatt
     1861 cccggggtgt gatattcctg tgcatcacca ggaaaaaatg ggcactatgg acccaacctt
     1921 taacccggtg atcaccgatg attccgccgc attcagtgaa caggcggtgg cggcaatgga
     1981 gaaagagctc agcaaactcc agcttaccga cagctaccag ctactggaaa aaatcgttaa
     2041 ctataaagat tcccctgcct gtaaagagaa acaacagtgt tcgctggtgg atggcaaaaa
     2101 tacctttagc gccaagtatc aacaagaacc aggtgtttcc gggccgctga aagtcggcaa
     2161 ctcgctggta gatgcgttta ctttgcaata ttacgaaggt tttccgatgg atcaggtggc
     2221 ctggggagaa atcaaatctg accagcagtg gaaggtgttg tcgaagctta aaaacggcta
     2281 ccaggacagc ctgtttacct caccggaagt ggcgcgcaat gttgcgaaac cgctggtcag
     2341 ttatatcgac aaagctctgg tcaccgatcg caccagcgca ccgaaaatta cagtgttggt
     2401 tgggcacgac tccaacattg cctctctgtt aacggcgctg gatttcaaac cgtatcagtt
     2461 gcatgaccag aacgaacgca cgccgattgg cggcaaaatc gttttccagc gttggcatga
     2521 cagcaaagcc aatcgcgatt tgatgaaaat tgaatatgtg tatcagagtg cggaacagtt
     2581 acgtaatgcc gatgcgttaa ccctgcaggc acctgctcag cgtgtgacgc tggaattaag
     2641 cggttgcccg atagatgcca acggtttctg cccgatggat aaatttgata gcgtgttgaa
     2701 tgaagcggtg aaataacaga aaactccccc gcgagaagcg ggggagtcgc tggttaaacg
     2761 tttttacgtt cgatggtctg ttcgccccaa aaaagcgaat ctttatcggc cttagcaaag
     2821 gctttgacta acacttcatc gctaccttct tcccaaatct tttctgccat tttttcgtcg
     2881 tacccggcga cttcgaaaat ggcctcggct atttccggcg acgtattgcg cagagatgcc
     2941 cattcaccga cgtgatgagc tttcgcttct tgagttggca tgcgtatcct cctgttgaag
     3001 attagccgtt aagtttaact gccagacctg cgacatattc cccttgataa cgagcaatag
     3061 acagttcttc ctggcttggc tggcgtgagc cgtcaccgcc tgcgatggtg gttgcgccgt
     3121 acggcgtacc gccgcgaacc tgtgaaacgt caaataattc ctgcgctgcg tagccaatgg
     3181 ggacaatcac catgccgtga tgcgcaaggg tcgtccaggt tgaagtaata gtttgttcct
     3241 gaccgccgcc agtaccggtg gaactaaaga cgctcgccag ttttccgtat agtgcgccgg
     3301 aagcccacag gccgcccgtc tggtcgagga aggtacgcat ttgaccggac atgttgccaa
     3361 agcgggtagg tgtaccaaaa ataatggcgt cgtaatcggc cagttcttgc ggggttgcaa
     3421 ccggtgcagt ttgcgtttta ccgcctgctt tttcaaataa ttgcggcggc atggtttccg
     3481 gtacacgctt aacgacaact tctgcgccat cgactttgct tgcaccctca gcgactgcgc
     3541 gtgccatcgt ttcaatatgt ccgtacatgg aataataaag cactagtatt cttgccttaa
     3601 cactcatatc attgttctcc tgttcaatga accacttaga taagcatagt ccgaagatat
     3661 gaagctgcta gtgacataca tcaggtggag tactaaagag caagactcag gagactcctt
     3721 aagacggcaa acaaacctca ttgatgcttt ctactctaag cacaaaaacg attattacct
     3781 tcttcctgct cacagatatg ttgataaggg taaatctggt tttcaccagc aacataagaa
     3841 tcaaggttct gacttcagac gtatgtttga aaatgttatg tctggggcta ttccagaagg
     3901 ttccttgatt gttgtagaga actttgaccg cttcagtagg gctgatatcg acacagctat
     3961 agatgatgtc aggcagatac tgaggaaggg ggtctctata ctgactctgg gtgatggtga
     4021 actctacgac aagtcagcct taacagaccc tgtgaagtt
//
LOCUS       DADMKF010000242         4045 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.246, whole genome shotgun sequence.
ACCESSION   DADMKF010000242 DADMKF010000000
VERSION     DADMKF010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4045
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.246"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            30..758
                     /gene="mpaA"
                     /locus_tag="JNC24_003949"
     CDS             30..758
                     /gene="mpaA"
                     /locus_tag="JNC24_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HAY9160568.1"
                     /translation="MTVTRPRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQAFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(733..1698)
                     /gene="ycjG"
                     /locus_tag="JNC24_003950"
     CDS             complement(733..1698)
                     /gene="ycjG"
                     /locus_tag="JNC24_003950"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HAY9160569.1"
                     /translation="MRTVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVSQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGVAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKIGGLTEALALATEARAQGFSLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            1817..2323
                     /gene="tpx"
                     /locus_tag="JNC24_003951"
     CDS             1817..2323
                     /gene="tpx"
                     /locus_tag="JNC24_003951"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HAY9160570.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTDVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(2367..3908)
                     /gene="tyrR"
                     /locus_tag="JNC24_003952"
     CDS             complement(2367..3908)
                     /gene="tyrR"
                     /locus_tag="JNC24_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HAY9160571.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICVTQKNLVELVQKGMFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVQRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
BASE COUNT          969 a         1142 c          970 g          964 t
ORIGIN      
        1 ctgtaatact tcctgaacca gagacgatca tgaccgtaac ccgcccacgc gccgaacgcg
       61 gcgcatttcc gcccggaaca gaacattacg gacgttcatt attgggcgcg ccgttaatct
      121 ggtttcctgc ccctgcagcc agccgcgaaa gtggtttgat tctggctggc actcacggtg
      181 atgaaaactc ttcggtcgtc acgctctcct gtgctctgcg gacgttgaca ccttctttgc
      241 gccgccatca tgtggtgctg tgtgtgaatc ctgacggctg ccagctgggg ttacgggcca
      301 atgctaatgg tgtggattta aacagaaact ttccggcggc gaactggaag gaaggtgaaa
      361 cggtttatcg ctggaacagc gccgctgaag aacgtgatgt cgtattactg acaggtgaca
      421 aacctggctc agaacctgaa acccaggcgc tgtgccagct tatacaccgc attcaacctg
      481 cctgggtggt ctcattccac gatccactgg cctgtattga agatcccaga cacagcgaat
      541 taggcgaatg gctggcccag gcgtttgaat tgcctctggt aaccagcgtc ggttatgaaa
      601 cgcctggctc tttcggcagt tggtgtgcag atctcaactt gcattgcatc accgcagagt
      661 ttccgcccat ctcctccgac gaagccagcg aaaaatatct ctttgcgatg gcaaacttgc
      721 tgcgctggca tcctaaagat gcaattcgcc cgtcgtgaac tgaagcgccg gttccacatc
      781 taccgccagc caggtcggtc cgtcaagatc ggcgaaactg acctgcggca ccagcggtaa
      841 agcggcgcta atggcacgag aggtacacaa catgcagccc agcatcagac tgaaaccttg
      901 tgcacgcgct tcagtcgcca gcgccagcgc ttccgtcaga ccaccgattt tatcgagctt
      961 aatgttaacc atctcatagc gccctttcag cgccttcaga ttgctacgtg tatgacaact
     1021 ctcatcagca caaatcggca acggatgaat aaaattctcc agcgccgcat catcctgcgc
     1081 aggaaggggt tgttcaagca tcgcaacgcc taaatccgcc aatagctggc aacgcgccgc
     1141 caccccttct gcacgccagg attcatttgc atcaacgatc agcgtcgcat cgggcacagc
     1201 tgtgcgaatt gccaccatcc gctcactaat aagatggtta tccagcttca ctttcagtaa
     1261 tttcgcgcct gcctgccaga gtgttgatgc actattggcc atctgatcag gcgtaccgat
     1321 gactaccgtc tgtgcagtga taactgtctc gggaagcgtt atgccgatca aatcagccag
     1381 cgattgctgc tgttttcgcg cggccagatc ccacaatgca caatccagcg cattacgtgc
     1441 tgcgccggca gggagaattt tttgcaactc ctcccgtgtc agccctttct ctaattgcga
     1501 cacgacgctc ataatttgcg ccattaccga ggcatcactt tctccataac gcggatacgg
     1561 cgtgcattcg ccggtgcctt taataccctc ttcttccagt tcaaccacca ccacgcgcgc
     1621 ttcactgcga cttccccggg caatcacaaa cggggtatgt aagggccagg cttcctcaaa
     1681 taccttaacg gttctcatct ttactccttg ttgtcgcggt ggtctgcgga ttaagcgcaa
     1741 atagcgtttg ctgtgttatt gacagttagc ataaactagg tgtgacgtta actatatgta
     1801 aacaggaaag ataatcatgt cacaaaccgt tcatttccag ggcaacccgg ttacagtcgc
     1861 caattccatc ccgcaggcgg gtagcaaagc gcagactttt actctcgtgg caaaagatct
     1921 gtctgacgtc accctcggtc agtttgcggg taaacgcaaa gtgctgaaca ttttcccgag
     1981 tattgatacc gatgtttgcg ccgcatcagt acgcaagttt aaccaactgg caactgagat
     2041 cgacaacacc gttgtgctgt gtatctctgc cgatctgccg ttcgcccagt ctcgtttctg
     2101 cggcgcagaa ggtctgaaca acgttatcac cctctccact ttccgtaacg ctgaatttct
     2161 gcaagcttac ggtgtggcaa ttgctgatgg cccactgaaa ggtctggcag cgcgtgccgt
     2221 tgtggttatt gacgaaaatg acaatgtgat tttcagccag ctggtggatg aaatcaccac
     2281 cgagccggat tacgaagcag ctctggctgt actgaaagca taagttaata tgcctgatgg
     2341 tgttgcacca tcaggcatat tcgcgcttac tcttcgttct tcttctgact cagaccatat
     2401 tcccgcaact tattggcaat cgcggtatgt gaaacgccga gacgttttgc cagtttgcgc
     2461 gtgctgggat aattgcgata aagctgggtt aataccgagc gttcaaaacg gctggtgatt
     2521 tcgtccagcg aaccttccat cgcatcttcg cccacggcta ccgttgcggc gtcataatcc
     2581 ggcaacaaaa tatcctgtgg acgcagctca taaccgtcca gttgtgtcag tgcgcgatag
     2641 atagcgttct ttaactgccg cacatttccc ggccacgcat aacgcgtaag tacagtattc
     2701 aggtcagcgg ccagtttcgg acgctgcacg ccctgctcgt cggcaaagcg ggcgacgaac
     2761 agctcagtta acggcatgat gtcctgcgga cagtcacgta gcggcggcag attgagcgtc
     2821 aacacgttca gacgataata gagatcttca cggaacatgc ctttttgcac cagttcgacc
     2881 agattcttct gcgtaacgca aatcacccgc acatcgacat gcacctcatg gtcttcgcca
     2941 acccgacgga aagtgccatc attaaggaaa cgcagtaatt tcgcctgcat ccgtggtgac
     3001 atttccccta tttcatccaa cagcaccgaa ccaccgttcg cctgctcaaa gaatcctttc
     3061 ttcccttccg gagcatgacc aaacagttca ctctcgaccg catcttccgg tatagacgca
     3121 cagttcagcg ccaggtaagg tttgcccgct ctggggcttg cctgatggca ggcgtaggca
     3181 aagagatctt tacctgtacc tgtgtcaccc gtaatcagca gcggcgcgct tagcatcgcc
     3241 agtttctgcg cctgttcgac aacatgcttc attttcgggc tgacggcgac aatttgactg
     3301 aaggcgctga cgtcctgggc ggcgacattt tgcaactggc ggcccatacg aatcgttgat
     3361 cgcaacatca ccaccgcacc ggtcaggacg tgttgatcat tttcatcctg aagataaaca
     3421 ggcgtaatct ccatcaggaa attctgccca ttaataacga catgctcgtt atgcgaatct
     3481 tgcggttcgc tttccagcca acgtaaaaaa ttaaagccgt taatcaattg tgcggcggta
     3541 tggttgcgca ggcgatccaa tttttgccca aaaagctgac agctcgccgg gttcgccata
     3601 tccactttgc ttttcatatc gacagagagc acaggttcag gcaacgcctc cagtaacgcg
     3661 ctcaacgcca gatgctcacg ttcggaaggc atccacggga cagtacgcac atcggtaaca
     3721 cccgcaatac ggcgtatttc ggccatcaga ctgctgaaac tctcaaactc cagttcagca
     3781 aaattgaggt agattcgccc aatgggatca atctcaatac cgcgtaaatc aatgcctctt
     3841 agcacgagta gatcgagtaa ttcgcgggtc agaccgagtc ggtcttcaca aaagacttcc
     3901 agacgcatgg gaaccttcac ctgaaaaaag aacaattaat atgatgatat gacactattt
     3961 ggatagcagg aaggtttctg tcaacaatca ttgacacaaa gacggaaaaa cgggcacttc
     4021 ggcgtaaaga tatccggtca gcacc
//
LOCUS       DADMKF010000243         3997 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.247, whole genome shotgun sequence.
ACCESSION   DADMKF010000243 DADMKF010000000
VERSION     DADMKF010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3997
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.247"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(1..2588)
                     /gene="acrF"
                     /locus_tag="JNC24_003953"
                     /pseudo
     CDS             complement(1..2588)
                     /gene="acrF"
                     /locus_tag="JNC24_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417732.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit AcrF"
     gene            complement(2600..3757)
                     /gene="acrE"
                     /locus_tag="JNC24_003954"
     CDS             complement(2600..3757)
                     /gene="acrE"
                     /locus_tag="JNC24_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrE"
                     /protein_id="HAY9160572.1"
                     /translation="MTKHARFFLLPSFILISAALIVGCNDKGEEKAHVGEPQVTVHIV
                     KTAPLEVKTELPGRTNAYRIAEVRPQVSGIVLNRNFTEGSDVQAGQSLYQIDPATYQA
                     NYDSAKGELAKSEAAAAIAHLTVKRYVPLVGTKYISQQEYDQAIADARQADAAVIAAK
                     ATVESARINLAYTKVTAPISGRIGKSTVTEGALVTNGQTTELATVQQLDPIYVDVTQS
                     SNDFMRLKQSVEQGNLHKENATSNVELVMENGQTYPLKGTLQFSDVTVDECTGSITLR
                     AVFPNPQHTLLPGMFVRARIDEGVQPDAILIPQQGVSRTPRGDATVLIVNDKSQVEAR
                     PVVASQAIGDKWLISEGLKSGDQVIVSGLQKARPGEQVKATTDTPADTASK"
BASE COUNT          981 a         1043 c          985 g          988 t
ORIGIN      
        1 tcctgatacg acatacctgt ccagtcataa ccaatgcccg caggtaattt tgacgcaagg
       61 ttttccatca atgccatggc atcgccggaa ctggttcctg gcgcggcttc cccctgaatc
      121 tccattgacg gcagaccgtt gtagcgttcc agtcgcggag agccatacac ccaatgtgaa
      181 gtggtaaagg cagagaatgg caccatttcg ccgttggcgc tgcggacata aagtttatcg
      241 acatcttctg gcagcatacg gaatttggca tccgcctgaa catacacctt tttcacgcga
      301 ccacggtcga tgaagtcgtt aacgtaagtc ccacccagcg ccgttgaaat ggtctgattg
      361 atgtcagaaa gtgaaacacc taatgcctgc gccttttcct ggtcaacttc cagtttaaac
      421 tgcgcggtgt cttccagacc attaggacgc acgctgacta agctggcagg atgttgcgcc
      481 gccatgccaa gcaactggtt acgggcctgg gtcagggcat cgtgacccag cccagcctga
      541 tcaattaact caaagtcgaa acccgttgcc gtgcccagtt caacaatggc tggcatattg
      601 aatggaatga caaaaccgtc gcggatcttg cccaattcca ttttggcacg atggattacc
      661 gcttccgcac tgttttcgtc accactacgc tcttcccacg gtttcagact gacgaaagcc
      721 ataccggcgt tttgtgcctg gccgctgaag ctaaagccgt taaccgtaaa gacactttct
      781 acgttcgctt tctcgttatt cagatttgat ccaacacttt ttgcgtccgc tcttgcgtcg
      841 cgccagcggg taactgaatc atggtcagaa agacaccctg atcctcttca ggtaagaagg
      901 aagaaggaag acggaagacg taaaaacaac accaccattc ctgcaacaat cagcgcatag
      961 atcagtaaat atcgtcctgt ggaaccgagg attttgccga cgctgttggt gtagtggtta
     1021 acgctatgat cgaaggtggt attaaaccag ccgaagaaac cgcccttatt ttcgtgatgc
     1081 tcagcagaga cgggtttaag cagcgttgca cataacgcag gggtaagaat caatgccacc
     1141 agaacagaaa gcgccattgc cgaaacgatg gtgatagaga actggcgata aattgcccca
     1201 gtagaaccgc cgaagaatgc catcggaata aataccgctg acagcaccat cgcgataccc
     1261 accagtgcgc cctgaatttg cgacatcgat ttttccgtcg cttcttttgg cgggagctta
     1321 tcctccatca tcacgcgctc gacgttctcc accaccacta tcgcatcatc gacgagcagc
     1381 ccgatggcaa gcaccatccc gaacatcgtt agtgtgttga tggagtaacc aaaagcggcg
     1441 aggatggcaa acgtccctaa caacaccacg ggtaccgcaa tggtggggat cagcgttgct
     1501 cgcatattct gcaagaacag atacatcacc aggaacacca gcataatggc ttcgaacagc
     1561 gtttttacca cttcgtgaat agaaagctgg acgaatggcg tggtgtcata agggtagaga
     1621 accttcattc cctgcgggaa gaatggctgt aattccgcca gttttgcctt aatggctttc
     1681 gcggtattga gagcgttcgc gccggttgct agcttaatcc ccaggcccgc cgccggtttt
     1741 ccgttgatac gagcgataac gttatagttt tcaccgccaa gttcaactcg tgcgacatct
     1801 ttcaggcgta ccaccgagcc gtcactgttt acgcgcaggg ttactttgcc gaattcttcc
     1861 ggatttttta accgcgtctg agcaataatc gaggcgttca actgttgccc tggtaacgct
     1921 ggcgttccgc ccaactgccc ggcagcgatc tgatcgttct gtaccttcaa ctggttaatc
     1981 acatcaaccg gtgtcagttt atatttgttt agcagatcgg cgtccagcca gatacgcatc
     2041 gcatactgtg cgccgaaaag ctgtacgtca ccgacgccat tcagacggct aagcgtatct
     2101 ttaacgttag aggccacata gtccgagata tcgtcctgtg tggtgtctgg gttatcagag
     2161 acaaagcccg ccaccatcaa atagctgcta ctggactttt caacactgat cccctgctgc
     2221 tgaacctcct gcggcagcaa cggcgtggcg agctggagtt tgttctgcac ctgcacttgc
     2281 gcgatatcag gatcggtccc ggactggaag gtaagggtaa ttgtcacgct accggcggaa
     2341 tcgctggtgg aggacatata catcaggtta tcgataccat tcatattctg ttcgataacc
     2401 tgcgtcaccg tatcctgcac ggtctgcgca tcagcacctg gatagtttgc ctaaacagaa
     2461 accgcaggcg gtgcaatggt tggatactga gcgacgggca attgtaggat cgccagtgcg
     2521 cccgccatca tcagaataat ggccagcacc catgcaaata tcggtcgtcg aataaaaaag
     2581 tttgccatgt cagattacct tacttcgatg cagtatctgc gggggtatcg gtagtggctt
     2641 taacctgctc tcccggacgc gctttttgca ggccgctgac aatgacttga tcgccagatt
     2701 tcagaccttc gctaatcaac catttatcgc caatcgcctg actggcaacg acagggcgcg
     2761 cttccacctg acttttatcg ttaacaatca gcacggttgc atcaccacgc ggtgtgcggc
     2821 taacgccttg ttgcgggata agaatggcgt caggttggac gccttcatca atccgtgcac
     2881 gcacaaacat acccggcaaa agcgtatgtt gcgggttagg gaagacagca cgtagggtta
     2941 tggagccggt gcattcatca acggtcacat cagagaattg cagcgtacct ttcaggggat
     3001 aggtttgacc gttttccatg accaactcta cgttgctggt ggcgttttcc ttatgcaaat
     3061 ttccttgctc tacggattgc ttcagcctca taaaatcgtt gctggattgg gtcacatcaa
     3121 cgtagatagg atcgagctgc tggacagtcg ccagttcagt cgtttgccca ttagtgacaa
     3181 gagcgccttc ggtcacggtc gatttgccga tacgtccgct aattggcgca gtcactttgg
     3241 tataagcgag attgatgcga gcgctttcga ctgtggcttt tgcggcaatc acggcggcat
     3301 cggcctggcg agcatcagca atggcctggt cgtactcctg ctggctgatg tatttcgtac
     3361 ccacgagcgg aacgtaacgt tttaccgtca aatgcgcgat ggcggcggcg gcttcacttt
     3421 tcgccagttc gcctttcgcg ctgtcataat ttgcctgata ggtcgcggga tcgatctggt
     3481 acagggactg gcctgcttgt acatcgctgc cttcagtgaa attacgattc agtacgatcc
     3541 cgctaacctg tgggcgaact tcggctatac ggtaagcatt ggtgcggcct ggtaattcag
     3601 tcttaacttc taacggggcc gttttcacaa tatgaacggt aacctgcggt tcaccgacgt
     3661 gagctttctc ttctccctta tcgttacaac cgacgattaa agccgcggag atcagaataa
     3721 aggagggcag gaggaaaaac ctggcatgtt tcgtcattac tattcctcaa aaaaacaaaa
     3781 gcgcgttatt tacccagaaa ggcaacgcgt taactcgcag aaagaaaaat acagtttgct
     3841 atcctacaaa ttatcattcg tcgatgtaag gaatagttat gaatactggc gtctcaaggc
     3901 acataaacac aaaaaaagat taatattcta ctgttttatt ttgacgcggg ttgaaagagg
     3961 cagaattaaa acctcgtaaa ttgaaatata tattcat
//
LOCUS       DADMKF010000244         3951 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.248, whole genome shotgun sequence.
ACCESSION   DADMKF010000244 DADMKF010000000
VERSION     DADMKF010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3951
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.248"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            705..1898
                     /locus_tag="JNC24_003955"
                     /pseudo
     CDS             705..1898
                     /locus_tag="JNC24_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001460468.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
     gene            1909..3261
                     /locus_tag="JNC24_003956"
     CDS             1909..3261
                     /locus_tag="JNC24_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001087757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9160573.1"
                     /translation="MNSNVLTQTIVTGSDPRGLPEFSAIREEINKASHPSQPELNWKL
                     VESLALAIFKANGVDLHTATYYTLARTRTQGLAGFCEGAELLAAMVSHDWDKFWPQGG
                     PARTEMLDWFNSRTGNILRQQISFAESDLPLIYRTERALQLICDKLQQVELKRVPRVE
                     NLLYFMQHTRKRLEPQPKSNAENAAQTTVRTLIYAPETLASSTPEAVVPPLPGLPEMK
                     VEVRSLTENPPQASVIKQGSTVRGFIAGIACSVAVASALWWWQVYPVQQQLLQVNDTA
                     QGAATVWMASPELENYERRLQQLLDTSPVQPLETGMQMMRVADSRWPESLQQQQASTQ
                     WNEALKTRAQSSPQLRGWLQTRQDLHAFADLVMQREKEGLTLSYIKNVIWQAERGLGQ
                     ETPIESLLTQYHDARAQKQNTDALEKQINERLEGVLSRWLLLKNNVMPEAATGTTAEK
                     "
     gene            3285..3767
                     /locus_tag="JNC24_003957"
     CDS             3285..3767
                     /locus_tag="JNC24_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HAY9160574.1"
                     /translation="MANISYLSLSGETQGLISAGCSTLDSVGNKAQPEHKDQIMVYAL
                     MHSISRSQNVNHHELIITKPVDKSSPLLAKALSDNEKMAICEFILYRTSKAGIYQPYY
                     KINLSKARISSIDFVTPHAVLEKELEPQERIAFIYEDISWEHTLAGTNAMSKWQDRVQ
                     "
BASE COUNT         1060 a         1019 c         1062 g          810 t
ORIGIN      
        1 tggaacagac ctatctgcaa ctgctggagc aacgttacct gccctcgctg tttaacgggc
       61 tggtcaaagc gatgaacgcc gcgccgccgg agagtgaaga aaaactcgcg gtgctgcgcg
      121 tgatgcgaat gctggaggac aaaagcggac gtaacaatca ggtggtgaag cagtatatgg
      181 caaaacgctg gagcgaaaag ttccacggtc agcgcgatat ccaggcacaa ctgatgtccc
      241 atcttgacta cgcgctggct catactgact ggcacgcaga gcgtcaggcg ggcgacggtg
      301 acgccatcag ccgctggacg ccatatgaca agcccgtggt atcagcacag aaagaactga
      361 gcaaactgcc tgtctaccag cgggtttacc agagcctgaa aacgcgggcg ctgggcgttc
      421 ttcctgccga cctcaatctg cgtgaccagg tagggccaac ctttgaccag gtgtttacat
      481 ctgccgatga caacaaactg gttgttccac agtttatcac ccgctacggc ctgcaaagct
      541 attttgtaaa acagcgcgat gaactggttg aactgacagc gatggattcc tgggtactta
      601 acctcacccg caatgtgaaa tacagtgacg ccgaccgtgc ggaaatccag caccagttga
      661 ccgagcagta tatcagcgac tacaccgcca cctggcgggc tgggatggac aatctgaata
      721 tccgcaattt tgagtccatc ggacaactga ccggggcgct ggagcaggtt atcagcggcg
      781 accagccttt gcagcgggcg ctgaccgtgc tgcgtgacag cacacagcca ggcgtctttt
      841 ctgaaaaact ctctgccaaa gaacgggagg aagccctggc agagccggat taccagttac
      901 tcacccgcct cgggcatgaa ttcgccccgg aaaacagtac cctggcagta cagaaagaca
      961 aagaaagcac gatgcaggcc gtgtatcagc aactcaccga gttgcaccgc tacctgctgg
     1021 caatccagaa cgcgcctgta ccagggaaat cggcgctgaa agccgtgcag ttacggcttg
     1081 atcagaacag cagcgatccg atattcgcca cccgccagat ggcaaaaacg ctgcctgctc
     1141 cgctcaaccg ctgggttggc agactgactg actaggcctg gcatgtggtg atggtggagg
     1201 ctgttcatta tatggaagtg gactggcgcg acagcgtggt gaaaccgttt aacgagcaac
     1261 tggcaaataa ctatccgttt aatccgcgtt ctgcacagga tgcctcactg gatgccttcg
     1321 aacgcttctt taaaccggat ggcatactgg ataccttcta ccagcagaac ctgaagctgt
     1381 ttatcgataa tgacctgagt ctggaggatg gcgataacaa cgtcattatt cgcgaagata
     1441 ttattgcgca actggaaact gcgcagaaaa tccgtgacat cttcttcagc aaacagaacg
     1501 gtctgggaac atcctttgcc gtggaaacgg tatcgctttc aggcaataaa cgccgcagtg
     1561 tactgaacct tgacagtcag ttagtcgatt acagccaggg ccgtaactat accgcccatc
     1621 tggtctggcc taacaacatg cgcgaaggca acgaaagtaa gctgacgctc atcggcacca
     1681 gcggcaacgc gccgcgcagt atcagcttca gcgggccgtg ggcgcagttc cgcctgttct
     1741 gggccggaca actgaccgga gtacaggatg gcaactttac cgtgcgcttt agcgtggacg
     1801 gtggcgcgat gacctaccgt gtgcataccg acacggaaga taacccgttc agcggtgggt
     1861 tgttcagcca gtttggtctg tcagacacac tgtactgagg aggcagggat gaatagtaac
     1921 gtactgacac aaactatcgt taccggcagt gacccgcgcg ggctgccgga attcagcgcc
     1981 atccgcgagg aaataaacaa agccagccac ccgtcacagc ctgagctgaa ctggaaactg
     2041 gtggagtcgc tggcgctggc gatttttaaa gccaacggtg tggatttaca caccgccacc
     2101 tactatacgc ttgcccggac acggacacag ggactggcgg gattctgcga aggtgcggaa
     2161 ctgctggcgg caatggtaag ccacgactgg gataagttct ggccgcaggg cggcccggcg
     2221 cgtactgaaa tgctggactg gtttaactcc cgaaccggca atattctgcg tcagcaaatc
     2281 tcctttgcgg aatccgacct accactgatc taccgcacag agcgggcatt gcagcttatc
     2341 tgcgataagc tccagcaggt ggaactgaag cgcgttccgc gcgtggagaa tctgctctat
     2401 tttatgcagc acacgcgtaa acggcttgaa ccccagccga agagtaacgc tgagaacgcc
     2461 gcacagacca cggtcagaac gctgatttat gccccggaaa cactggcatc ttccacacca
     2521 gaagcggtag tgcctcccct gcccggcctg cctgagatga aagtggaagt gcgcagtctg
     2581 acagagaatc ccccacaggc cagtgtgata aagcaaggca gtacggtaag agggtttatc
     2641 gcagggatcg cctgttcagt ggctgtcgcc tcagcattgt ggtggtggca ggtctatccg
     2701 gtgcagcagc aactgttaca ggttaacgac accgctcagg gcgcagcaac ggtgtggatg
     2761 gcctcacctg aactcgaaaa ctatgagcgc aggctgcaac aacttcttga tacctccccg
     2821 gtacagccgc tggaaaccgg gatgcagatg atgcgtgttg ccgacagtcg ctggccggaa
     2881 agcctgcaac agcaacaggc ctcgacacaa tggaatgagg cactcaaaac ccgcgcacag
     2941 agtagcccgc agttgcgtgg ctggttgcag acccgccagg acttacatgc ttttgcagat
     3001 ctagtgatgc agcgcgagaa agagggacta accctttcct atatcaaaaa tgtcatctgg
     3061 caggcggagc ggggactggg gcaggaaacc cccattgagt ctctgttgac gcagtaccat
     3121 gatgcccgtg cgcagaagca gaatacagat gcgctggaaa aacaaattaa tgagcgactc
     3181 gaaggcgtgt taagccgctg gctgctgctg aagaataacg tcatgccaga ggcggcaacc
     3241 ggtaccacgg ccgaaaaata attatttaat taaaggagat tatcatggca aatataagtt
     3301 atttatcttt gagtggagag acacaagggc taatttcagc aggttgctca acgcttgatt
     3361 ctgtcggaaa caaagcgcag ccagaacata aagatcagat catggtttat gcgttaatgc
     3421 attccatctc aagaagtcaa aatgtcaatc atcatgaatt aattatcacc aaaccggttg
     3481 ataaatcctc accattgctg gcaaaagcac ttagtgacaa tgaaaaaatg gcaatatgtg
     3541 aatttatact ctaccgaacc agtaaagcgg gtatttacca gccttattat aaaataaatc
     3601 tgtcaaaagc ccgaatatca agtattgatt ttgtcacacc gcatgctgtc ctggaaaaag
     3661 aactggaacc acaggagaga attgccttta tttatgaaga tatttcctgg gagcatacgt
     3721 tagccggtac gaatgcaatg agtaaatggc aggatcgtgt acagtaactt actgtaataa
     3781 cataataaat tactattgcg gagagataaa atggctggtt taaggaataa caacaacaca
     3841 cagaatgcac agtgggctga ttatgtgggt gacattcttc ggggggctca gccgataaac
     3901 cagctagtac ctcagcaccc ttatcttaac gacgtcccac tcattgatga g
//
LOCUS       DADMKF010000245         3950 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.249, whole genome shotgun sequence.
ACCESSION   DADMKF010000245 DADMKF010000000
VERSION     DADMKF010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3950
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.249"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(<1..953)
                     /gene="ydcO"
                     /locus_tag="JNC24_003958"
     CDS             complement(<1..953)
                     /gene="ydcO"
                     /locus_tag="JNC24_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BenE family transporter YdcO"
                     /protein_id="HAY9160575.1"
                     /translation="MLRCLYAITHEVTMRLFSIPPPTLLAGFLAVLIGYASSAAIIWQ
                     AAIVAGATTAQISGWMTALGLAMGVSTLTLTLWYRVPVLTAWSTPGAALLVTGLQGLT
                     LNEAIGVFIVTNALIVLCGITGLFARLMRIIPHSLAAAMLAGILLRFGLQAFASLDGQ
                     FTLCGSMLLVWLATKAVAPRYAVIAAMIIGIVIVIAQGDVVTTDVVFKPVLPTYITPD
                     FSFAHSLSVALPLFLVTMASQNAPGIAAMKAAGYSAPVSPLIVFTGLLALVFSPFGVY
                     SVGIAAITAAICQSPEAHPDKDQRWLAAAVAGIFYLLAGLFG"
     gene            1006..1542
                     /locus_tag="JNC24_003959"
     CDS             1006..1542
                     /locus_tag="JNC24_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAY9160576.1"
                     /translation="MENLARFLSTTLKQLRQQRGWSLSRLAEATGVSKAMLGQIERNE
                     SSPTVATLWKIATGLNVPFSTFISPPQSAMPSVYDPQQQAMVITSLFPYDPQLCFEHF
                     SIQMASGAISESTPHEKGVIEHVVVIDGQLDLCVDGEWQTLNCGEGVRFAADVTHIYR
                     NGGEQTVHFHSLIHYPRS"
     gene            1615..3576
                     /gene="rlhA"
                     /locus_tag="JNC24_003960"
     CDS             1615..3576
                     /gene="rlhA"
                     /locus_tag="JNC24_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA 5-hydroxycytidine C2501 synthase"
                     /protein_id="HAY9160577.1"
                     /translation="MTVSSHRLELLSPARDAAIAREAILHGADAVYIGGPGFGARHNA
                     SNSLKDIAELVPFAHRYGAKIFVTLNTILHDDELEPAQRLITDLYQTGVDALIVQDMG
                     ILELDIPPIELHASTQCDIRTVEKAKFLSDVGFTQIVLARELNLDQIRAIHQATDATI
                     EFFIHGALCVAYSGQCYISHAQTGRSANRGDCSQACRLPYTLKDDQGRVVSYEKHLLS
                     MKDNDQTANLGALIDAGVRSFKIEGRYKDMSYVKNITAHYRQMLDAIIEERGDLTRAS
                     SGRTEHFFVPSTEKTFHRGSTDYFVNARKGDIGAFDSPKFIGLPVGEVLKVAKDHLNV
                     AVTEPLANGDGLNVMIKREVIGFRANTVEKTGENQYRVWPNEMPADLHKIRPHHPLNR
                     NLDHNWQQALTKTSSERRVAVDIELGGWQEQLILTLTSEEGVSITHTLDGQFDEANNA
                     EKAMNNLKDGLAKLGQTLYYARDVQINLPGALFVPNSLLNQFRREAADMLDAARLASY
                     QRGSRKPVADPAPVFPQTHLSFLANVYNQKAREFYHRYGVQLIDAAYEAHEEKGEVPV
                     MITKHCLRFAFNLCPKQAKGNIKSWKATPMQLVNGDEVLTLKFDCRPCEMHVIGKIKN
                     HILKMPLPGSVVASVSPDELLKTLPKRKG"
     gene            complement(3668..3898)
                     /locus_tag="JNC24_003961"
     CDS             complement(3668..3898)
                     /locus_tag="JNC24_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncJ family protein"
                     /protein_id="HAY9160578.1"
                     /translation="MFTKALSVVLLTCALFSGQLMAGHKGHEFVWVKNVDHQLRHEAD
                     SDELRAVAEESAEGLREHFYWQKSRKPEAGQR"
BASE COUNT         1023 a         1008 c         1001 g          918 t
ORIGIN      
        1 ccaaacagac ctgcgagcaa atagaaaatg cctgcaacgg cagctgccag ccaacgttga
       61 tctttatccg gatgcgcttc cgggctttgg caaatagccg cggtgattgc cgcaataccg
      121 acggaataaa cgccgaaagg ggaaaaaacc agtgccagca atccagtaaa tacaattaat
      181 ggcgaaacag gagccgaata tccagctgct ttcattgctg cgatacccgg tgcgttttgc
      241 gatgccatcg tcaccagaaa aagggggagt gcaacgctca ggctgtgagc aaacgaaaaa
      301 tcaggggtaa tataagtggg gagaacgggt ttaaagacaa catcagttgt gacaacgtca
      361 ccttgcgcga taacgatcac gatcccaata atcatcgcgg caattaccgc atagcgcggc
      421 gcaacggcct tggttgccag ccataccagc aacatacttc cacacaacgt aaattgaccg
      481 tccagactgg caaacgcctg taaaccaaag cgtaataaaa tcccggcaag cattgccgcc
      541 gcaagcgagt gcggaataat gcgcatcaga cgagcaaaga gtcccgttat gccgcagagg
      601 actattaacg cgttggtgac aataaaaacg ccgatggctt cgttaagtgt tagtccctgc
      661 aatccggtga ccaacaaagc cgcgccaggc gttgaccatg cggtgagaac aggtacgcga
      721 taccataatg tcagagtcag cgtactgacg cccattgcca gccccagcgc cgtcatccag
      781 ccagagattt gtgcagtggt ggctccggcg acaatcgctg cttgccagat tattgccgct
      841 gaactggcgt agccaattaa taccgccaga aaccccgcca gtagcgtggg tggaggaata
      901 gaaaacagac gcatagtcac ctcgtgcgtt atagcgtaca ggcaccgtag catttgtccg
      961 ttataacgca caagtgataa acttccgttt tgccggagga gtcgcatgga aaatctcgct
     1021 cgctttttat ccactacact taaacaacta cgccagcagc gcggctggag tctttcgcga
     1081 ctggcagaag cgacgggcgt ttctaaagca atgttagggc agattgagcg taatgagtcc
     1141 agtccgacgg tcgcgacatt atggaaaatt gccaccgggt tgaacgtacc attttcaaca
     1201 tttatttctc cgccgcagtc cgctatgcct tcagtttatg atccacaaca gcaggcgatg
     1261 gtgatcactt cgctgtttcc ttatgatcca caactctgct ttgaacactt ctcgattcag
     1321 atggcatctg gtgcaataag cgaatcgacg ccccatgaga agggggtaat cgaacatgtc
     1381 gtcgtcattg atggacaact tgatctgtgc gttgatgggg aatggcagac ccttaattgt
     1441 ggggaaggcg ttcgatttgc tgcagacgtc acgcatatct atcgtaatgg tggggagcaa
     1501 accgtacatt ttcattccct catccattac ccgcgcagtt aagcgggaaa actatttcgc
     1561 aacgtcgcgc ttgtggctaa aatagccgcc atttttcagc tactggataa gaatgtgacc
     1621 gtatcttctc atcgacttga actgttaagc ccggcacgcg atgccgccat tgcccgcgaa
     1681 gctattttgc acggtgccga tgctgtttat atcggcggcc ctggttttgg tgcccgtcat
     1741 aatgccagta atagcctgaa agatattgcc gagctggtgc cgtttgccca tcgttatggt
     1801 gcaaaaattt tcgtcacgct taacaccatt ttgcatgatg atgagctgga acccgcgcaa
     1861 cggctgatta ctgacctcta ccagaccggt gtcgatgcgc tgattgttca ggatatgggg
     1921 attctggaac ttgatattcc gccgattgaa ctgcacgcca gtacgcagtg cgacattcgt
     1981 acagttgaaa aagcgaagtt cctctctgat gttggcttca cgcagattgt gctggcgcga
     2041 gagctgaatc ttgatcagat ccgcgcgatt caccaggcta cggacgcgac cattgaattc
     2101 tttattcatg gggcactgtg cgtggcctat tcgggtcagt gctacatttc tcatgcgcaa
     2161 acagggcgta gcgccaaccg tggcgattgc tcgcaggcgt gccgtttgcc atacacattg
     2221 aaagacgatc aggggcgggt ggtttcctat gaaaaacatc tgctgtcgat gaaagataac
     2281 gatcagactg ccaacctcgg cgcgctgatt gatgctggcg tacgctcctt caagattgaa
     2341 gggcgttaca aagatatgag ctacgtgaag aatatcaccg cccattatcg ccagatgctg
     2401 gatgccatta ttgaagaacg tggcgatctg acgcgtgctt catcaggtcg tactgaacat
     2461 ttctttgttc catcgacgga aaagactttc caccgtggta gcacagatta ttttgtgaat
     2521 gcccgtaaag gcgatattgg cgcgttcgat tcgccgaaat ttatcggcct gccggtgggt
     2581 gaagtattga aagtggcgaa agatcatctc aatgttgccg tcaccgagcc actggcaaat
     2641 ggtgatggcc tgaacgtgat gattaaacgt gaagtcatcg gttttcgtgc caatacggtc
     2701 gagaaaaccg gagaaaatca gtaccgcgtc tggcccaatg aaatgccagc agatttgcac
     2761 aaaattcgtc cacatcaccc actaaaccgt aatcttgatc ataactggca gcaggcactg
     2821 acaaaaacct ccagcgaacg tcgggtggcg gtagacattg aactgggcgg ctggcaggaa
     2881 caactgattc tgaccctcac cagtgaagag ggtgtcagca tcacgcatac gctggacggg
     2941 cagttcgacg aagccaataa cgccgaaaaa gcaatgaaca atctgaagga tggtctggca
     3001 aaactggggc aaaccctcta ttacgcccgc gatgtgcaaa ttaatttgcc gggggcgctg
     3061 tttgtaccaa acagtctgtt aaaccagttc cgccgtgaag ctgctgacat gctggatgct
     3121 gcgcgtcttg ccagttacca gcgcggcagc cgtaaaccgg ttgctgatcc tgcgccggtt
     3181 tttccgcaaa cgcatctgag tttcctcgcg aacgtataca accagaaagc gcgtgaattt
     3241 tatcatcgct atggtgtgca gctgattgac gcggcgtatg aggcacatga agagaagggc
     3301 gaagtcccgg tgatgatcac caagcattgt ctgcgctttg cctttaatct gtgcccgaaa
     3361 caggcgaaag gcaatatcaa aagctggaag gcgacgccaa tgcaactggt taacggcgat
     3421 gaagtattaa cgctaaagtt tgattgccgc ccatgcgaga tgcacgtcat tggcaaaatc
     3481 aaaaatcaca tactgaaaat gccgttaccg ggaagcgtag tggcatccgt aagtccggat
     3541 gagctgctga aaacattgcc taagcgaaaa gggtaaaacg ccagttttct ggttactcac
     3601 aactcattga atctgcatga tattgcctgc cgggtaaggc gtcacgccgc atccggcatc
     3661 aaacgactca acgttgtccc gcttctggtt tgcgcgattt ttgccagtaa aaatgctcgc
     3721 gcaaaccttc cgccgactct tccgccacag cacgcaattc atcgctgtcc gcttcatgac
     3781 gcagctgatg atccacattc tttacccaca caaattcatg tcctttgtgc cctgccatga
     3841 gttgtcctga gaacagcgca cacgttaata agacaaccga taacgccttc gtaaacatcc
     3901 tgccaccttt ttcttacctt ttgccgctat gatgccgatc gtttcttgag
//
LOCUS       DADMKF010000246         3915 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.250, whole genome shotgun sequence.
ACCESSION   DADMKF010000246 DADMKF010000000
VERSION     DADMKF010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3915
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.250"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            <1..301
                     /locus_tag="JNC24_003962"
     CDS             <1..301
                     /locus_tag="JNC24_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9160579.1"
                     /translation="RVELYYRFNHEEEYYPSHRFMLVVDMKTVMVFSQRAESLGLYSN
                     NRLLINIIAEHIHSDIYLTKYQQQYPDANALINTLHESGNDMVQDEKHLSDSKNR"
     gene            390..2258
                     /locus_tag="JNC24_003963"
     CDS             390..2258
                     /locus_tag="JNC24_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001548650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAY9160580.1"
                     /translation="MIFFNDIKPTGWLKNKMEWYMNGHIGELDKLVPHLITEHKIYGR
                     DRITPRTVSAEDGVIKKQNNPDDNMMQYYWWDSETQSNWKDGYCRSAYLLDNKEWQEK
                     ASDLCLELIDTQDDDGYIGIYDSSLKFNCKAENGEFWAQATALRFVLGCYESTRNESL
                     LTSLTQAAQCIMAGYPKETSHPFVVEQGFAGHSHGLTITDVFYRLYEITNNIEYLSYC
                     LWLYEDYSAGCVSEQDLQYKNVIDPGYLLKGHGVHTYEHIRALAIAASQRPQYQTALD
                     IFLSQLKYYLTPSGAPIGDEWITGRTANATYTGYEYCSVHELFSTYVMLIKLTTNIGL
                     ADDAEWLFYNASFGMQHPILHAIMYCKTDNCYEANERKHPDSNQFNTRYKYSPTHEEA
                     AVCCVPNTARTIPAFVENMFQENNNGFTALFYGPCEFYGTFNNVAIKITQLTEYPHDL
                     SVKCLIEPESSVRFALSFRYPGWAKMMIINGVTFTKNDTQNSLIILDRTWQHHDVIDI
                     KFIADIQFNTDLCGDTYISRGPVLYAIELESDILIKKNLLNGKYFDSGYIPCSRADES
                     IQFSYADMESFSYSTSPEGKQYIEGCFYLNKTKIVRKLIPFGQTILRKVTFSNIES"
     gene            2295..>3915
                     /locus_tag="JNC24_003964"
     CDS             2295..>3915
                     /locus_tag="JNC24_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAY9160581.1"
                     /translation="MYLTDNIIKLSNKIGQRVYTVVSEMSIEAWITKEPQPFVYRRSG
                     AYQKLTIGSDWGQLFDCAWMRVYGKRPADKFRHKLVALVDIGGEGLIVDKNGSPICGI
                     TNKASSYGVPPDKPGKWVVDLSLVSENDEVEFWIDAACNDLFGYVTNGGIITDVHIAT
                     RNQLLKSLYYDIEVLFDWINDGQKFESIHPKGITPEQFIAKRSERTDEIISTLEYIDN
                     TLITFSNEEIIKCQIAIQSIINKNNNPSEFRIMATGHAHLDIAWMWPLREGRRKAIRT
                     FATALANIEKYPDYIFGASQYQLFHWIKKDYPYFFEKLKKQIAAGRFELQGCFWVECD
                     LNLVSGESLIRQIMHGTRFTLQNFSKKINYVWQPDVFGFPATLPQILKKSGINYIASQ
                     KLSQNKINKFNNYLFRWQGLDGSEILMHNFPEDTYDSRARARSLEYIEQNYNEKEICP
                     YALMVYGVGDGGAGPGEEHIERLTRIRNIDGLPHVDFSRVDKFFTYADAFRESLPIIS
                     GELYFEAHQGCFTSESATKAHNRNMENKLHDA"
BASE COUNT         1230 a          721 c          766 g         1198 t
ORIGIN      
        1 acgtgtagaa ctgtattacc gctttaacca tgaagaagaa tattaccctt ctcaccgttt
       61 tatgcttgtt gttgatatga agacagtaat ggttttttca caacgagctg aatcactggg
      121 gttatactcg aataatcgct tgcttatcaa tataattgcc gaacatatcc atagtgatat
      181 atatctaacc aagtatcagc aacaatatcc agatgccaat gcattaataa atactttgca
      241 tgaatctggc aatgatatgg tgcaggatga aaaacatttg agtgatagca aaaaccgtta
      301 atattttcct ttcattgtga tcgtctgtag gcgatcacaa tctatttgtc ttttataacg
      361 tctaaacttt tttatgcaaa ggtgtattta tgattttttt taacgatata aagcccactg
      421 gttggttaaa gaataagatg gaatggtata tgaacggtca tataggagaa ctggataaat
      481 tagttcccca tcttattacg gagcataaaa tatatggaag agatcgtata actccacgca
      541 ctgtatctgc tgaagatggc gttattaaaa aacagaataa tcccgatgat aacatgatgc
      601 aatattattg gtgggatagt gaaactcaat cgaattggaa ggatggatat tgccgcagtg
      661 cctacttgtt agataataaa gaatggcagg aaaaagcgag cgatttatgt ttggaactta
      721 tcgatacgca agatgatgat ggctacattg gaatttatga ttcatcatta aaatttaact
      781 gtaaagcaga aaacggagag ttctgggcac aagctactgc cttgcgtttt gtgcttggat
      841 gttatgagtc cacccgtaat gagagtttat taacttctct tacccaagcc gcacaatgca
      901 ttatggctgg ctaccctaaa gaaacgtcac atcctttcgt tgtggaacag ggttttgctg
      961 gacatagcca cggattaaca ataacagatg ttttctaccg cctttatgaa ataaccaata
     1021 acattgaata tctctcatat tgcttgtggc tgtatgaaga ttattccgca ggatgcgtgt
     1081 cggagcaaga tctgcaatat aaaaatgtca tagaccctgg ttaccttctt aaaggtcatg
     1141 gcgtacatac gtatgagcat attagggcat tagccatcgc agcgagccaa cggccgcagt
     1201 atcaaactgc attagatatt tttttatccc agttgaaata ttatcttaca ccatctggcg
     1261 ctcccattgg tgatgagtgg attacaggac gcacagctaa tgccacttat acaggctatg
     1321 aatattgttc tgttcatgaa ctatttagca cttatgttat gttaataaaa ctgaccacta
     1381 atattggtct ggccgatgat gctgagtggc tattctataa tgctagtttt ggcatgcagc
     1441 atcccatact tcacgcgata atgtactgta agactgataa ttgttatgaa gcaaatgaaa
     1501 ggaaacatcc tgacagtaat cagtttaata ccagatataa atactcccct actcatgagg
     1561 aggctgctgt ctgctgcgta ccgaatacag caagaaccat accggctttt gtcgaaaata
     1621 tgtttcaaga aaataacaat ggttttactg cattatttta tggcccgtgt gagttttatg
     1681 gcacatttaa taatgttgca attaaaatta cacaattaac tgaataccct catgatttaa
     1741 gcgtaaaatg tctcattgag cctgagtcat ctgttcgttt tgctctcagt ttccgttacc
     1801 ccggctgggc aaaaatgatg attattaatg gtgttacttt tactaaaaat gatactcaaa
     1861 actcacttat tattttagat agaacatggc aacatcatga tgtgattgac attaaattta
     1921 tagctgatat acagtttaat actgacttat gtggtgacac ttatatttcc agaggtcccg
     1981 ttttatatgc aattgagctt gagagcgata ttttaatcaa aaaaaacctg ctgaacggga
     2041 aatattttga ctcaggatac ataccctgta gtagagctga tgaatctatc cagttctctt
     2101 atgctgatat ggaatctttt tcatattcaa cctcaccaga gggcaaacaa tacattgaag
     2161 gctgttttta tttaaacaag actaaaatag tcagaaaact tataccattt ggtcaaacca
     2221 ttcttcgcaa agtaactttc tccaatatag agagttaata ttttcgagtg ttataattaa
     2281 ttaaagggat ttctatgtac cttactgata atattatcaa actgtcgaat aaaataggtc
     2341 agcgtgttta tactgttgtc tctgaaatgt cgattgaagc atggataaca aaagaaccgc
     2401 agccttttgt ttatcgccgt agcggcgctt accagaaact caccattggc tcagactggg
     2461 gtcaactgtt tgactgtgcc tggatgcgag tttatggtaa acgtccagca gataaattta
     2521 gacacaaact cgtcgctctc gttgatatcg gtggcgaagg tcttattgtg gataagaatg
     2581 gttcacccat ctgcggcatc acgaataaag catcatctta tggcgtaccg ccagataaac
     2641 caggtaaatg ggtggttgac ctttctctcg ttagtgaaaa tgacgaggtt gagttttgga
     2701 ttgatgctgc ctgcaacgat ttatttggct atgttactaa tggagggatt attactgatg
     2761 ttcatatcgc gacacgtaat caactactga aatcccttta ttatgatatt gaggtgcttt
     2821 tcgactggat taatgatggt cagaagtttg aatccataca tcctaaaggt atcaccccag
     2881 aacaatttat tgccaaaaga agtgagcgta ctgacgaaat cataagtact ctcgaatata
     2941 ttgataatac gttaattacc tttagcaatg aagaaataat taaatgtcaa attgcaatcc
     3001 aatccataat caataaaaac aataatcctt cagagttcag gattatggct actggtcatg
     3061 cccatttaga tatagcgtgg atgtggcctt taagagaagg acgcagaaaa gcaataagaa
     3121 catttgccac cgcccttgca aacattgaaa aatatcctga ttacattttt ggcgctagtc
     3181 agtaccaact ttttcactgg ataaaaaaag attatcccta tttcttcgaa aagttaaaaa
     3241 aacaaatcgc cgcaggccgc tttgagttac aaggttgctt ctgggtcgaa tgcgatttaa
     3301 atctggtaag tggtgaatcg ctgataaggc aaatcatgca tgggacacga ttcacactgc
     3361 agaatttttc aaaaaagata aattatgtct ggcaaccgga tgtctttgga ttccccgcaa
     3421 cgcttccaca aattctcaaa aaatcaggca ttaactatat tgcatcgcaa aagctctcac
     3481 aaaacaagat taataaattt aataattacc ttttccgctg gcagggcctg gatggctcag
     3541 aaattctgat gcataacttt cctgaagaca cttatgatag ccgagcacgt gctcgctctt
     3601 tagaatatat tgaacaaaac tataatgaaa aagagatctg tccctatgcg cttatggtgt
     3661 atggtgtcgg tgatggcggc gctggccccg gtgaggaaca tatagaacga ttaacacgta
     3721 tacgcaatat tgatggctta cctcatgttg atttcagccg tgttgataag ttcttcactt
     3781 acgccgacgc ctttcgcgaa agcttgccca ttatttcagg tgaactttat ttcgaggctc
     3841 atcaagggtg ctttacatca gagtcagcaa ctaaagctca taatcgaaat atggaaaata
     3901 aattacatga tgctg
//
LOCUS       DADMKF010000247         3908 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.251, whole genome shotgun sequence.
ACCESSION   DADMKF010000247 DADMKF010000000
VERSION     DADMKF010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3908
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.251"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            24..590
                     /locus_tag="JNC24_003965"
     CDS             24..590
                     /locus_tag="JNC24_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HAY9160582.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            594..1169
                     /locus_tag="JNC24_003966"
     CDS             594..1169
                     /locus_tag="JNC24_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HAY9160583.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTAKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(1211..2263)
                     /locus_tag="JNC24_003967"
     CDS             complement(1211..2263)
                     /locus_tag="JNC24_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HAY9160584.1"
                     /translation="MPVLHNRISNDALKAKMLVESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            2488..3408
                     /locus_tag="JNC24_003968"
     CDS             2488..3408
                     /locus_tag="JNC24_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxL/LpxP family Kdo(2)-lipid IV(A)
                     lauroyl/palmitoleoyl acyltransferasee"
                     /protein_id="HAY9160585.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPVIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGIQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            3580..3777
                     /locus_tag="JNC24_003969"
     CDS             3580..3777
                     /locus_tag="JNC24_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HAY9160586.1"
                     /translation="MSPCGNDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQL
                     GVTGHSALNMWSGIVFSIIPS"
BASE COUNT          971 a          988 c          950 g          999 t
ORIGIN      
        1 gcaaattcgt attggatgca cttatgtcat tcacaaatac ccctgaacgc tatggtgtta
       61 tatctgcggc cttccattgg ctaagcgcaa ttattgtcta cggcatgttt gctttaggtc
      121 tgtggatggt cacgctcagt tattacgatg gctggtatca caaagcaccc gaactgcata
      181 aaagcatcgg tattttgtta atgatggggc tggttattcg cgttctgtgg cgtgtcatat
      241 ctccaccgcc gggtccgctg ccgagttatt cgcccatgac tcgtcttgcc gcaaaggctg
      301 gacatctcgc attgtaccta ttgctgttcg ccatcggcat cagcggctat ctgatctcaa
      361 ctgccgatgg taaaccgatc agcgtttttg gctggtttga cgtccccgcg acccttgccg
      421 acgctggcgc acaggcagac tttgccggtg ccctgcattt ttggctcgcg tggagcgtcg
      481 tagtactgtc cgttatgcac ggatttatgg ccctgaagca tcatttcatc gataaagacg
      541 acactctgaa gcgcatgctg ggaaagtcgt catctgacta tggagtataa aaaatgaaaa
      601 aaagcctgct tggtttaacc ttcgcgtccc tgatgttctc tgccggttca gcggttgccg
      661 ccgattacaa aattgacaaa gaaggtcagc acgcctttgt taatttccgc atccagcacc
      721 ttggctatag ctggttatac ggcaccttta aagatttcga cggtactttt acctttgacg
      781 aaaaaaatcc ggctgccgat aaagtgaatg tgacaattaa caccaccagc gtcgatacta
      841 atcacgccga acgcgataaa catcttcgca gtgcagattt cctcaatacc gcaaaatatc
      901 cacaggcaac attcacctcc accagcgtga agaaagacgg tgacgaactg gatattaccg
      961 gcgatctgac gctgaatggc gtaaccaaac ccgtcacgct ggaagcgaaa ttaattggtc
     1021 agggcgacga cccatggggt ggtaaacgtg caggcttcga ggccgaaggc aaaattaagc
     1081 tcaaggactt caatatcaag acagatttag gtccagcttc tcaggaagtg gatctgatta
     1141 tttcagtgga aggcgtacag cagaagtaag cgtgaaatgt tgccggatgg cacgcatccg
     1201 gcaatgatat ttattctgtt ggatcaggaa tgcccagtgt tgtattcaga cgtccacgag
     1261 acttattaaa gatcttattg ccattttcac gtcctgcccg acgccgtcgc tgttcttctg
     1321 gcggtaacgc gctttcttcg cagcaaatct cactacaaca acctttgtat ttttccgcgc
     1381 atactggaca ctgaataaac agcaggtggc agccatcatt tttacagttg gtatggctgt
     1441 cgcacggcgc gccgcactgg tggcaatgcg cgataatctc atccgatata cgttcgccca
     1501 tccgctcgtc aaaaacaaaa tttttgccaa taaaacgcac cggcaagccc tgctcgcgcg
     1561 ccttacgggc gtattcaata attccgccct cgatatgcca gactttgttg aatccgttat
     1621 gtttcatcca ggcactggcc ttttcacaac gaatgccgcc ggtgcagtac atgacgattt
     1681 ttttatcttt atgtgcctgc atcatctcga ctgcttttgg cagctgctca cggaaggtat
     1741 ctgccggaat ttcgagcgcg ttttcaaagt gccccacttc atactcatag tggttacgca
     1801 tgtcgataaa tagtgcatcg ggatcgtcaa gcatggcgtt cacttccgcc gcttgcagat
     1861 actcaccaac attgctggca tcaaagtgag gatcgtcaat accgtcggca acaatgcgat
     1921 cgcgtacctt catgcgcagt acccagaagg atttcccgtc atcatccaac gcgatattca
     1981 ggcgtaaacc ctctaaagcc gggtcgaagg catagagctg cgcgcgaaat gtttcaacat
     2041 tgctcgcagg tacgctgatt tgcgcgttaa tgccctcatg cgccagatac actcgcccaa
     2101 aaacattcag cgcggtaaac agctgatata aagcgtcacg ggtcgcctta ggatcggcga
     2161 tgtggaaata cttgtaaaac gaaatggtgg ttcgcggttc gctctcaacc aacattttgg
     2221 cttttaacgc gtcgttggaa atgcggttgt gtaacactgg catggtgtac ggttcctgcg
     2281 agatgggaaa gtaaaaatcc gcggcatgat atagcaatta tcgataatta acatccacac
     2341 attttacgct acatttgcgc attaaaaatt atttgttatt tacaagcgcg gcaatttcgc
     2401 ccagtcttca gccacaaatt ttggttgcgg gcgaaaaaat gcgacaatac atacaattgc
     2461 ccgaataggt tgaaaaacag gattgatatg acgaatctac ccaagttctc caccgcactg
     2521 cttcatccgc gttattggtt aacctggttg ggtattggcg tactttggtt agtcgtgcaa
     2581 ttgccctacc cggttatcta ccgcctcggt tgtggattag gaaaactggc gttacgtttt
     2641 atgaaacgac gcgcaaaaat tgtgcatcgc aacctggaac tgtgcttccc ggaaatgagc
     2701 gaacaagaac gccgtaaaat ggtggtgaag aatttcgaat ccgttggcat gggactgatg
     2761 gaaaccggta tggcgtggtt ctggccggac cgccgaatcg cccgctggac ggaagtgatc
     2821 ggcatggaac acattcgtga cgtgcaggcg caaaaacgcg gcatcctgtt agttggcatc
     2881 cattttctga cactggaact gggcgcgcgg cagtttggta ttcaggaacc ggggattggc
     2941 gtttaccgcc cgaacgataa tccactgatt gactggctac aaacctgggg ccgtttgcgc
     3001 tcaaataaat cgatgctcga ccgcaaagat ttaaaaggca tgattaaagc cctgaaaaaa
     3061 ggcgaagtgg tctggtacgc accggatcat gattacggcc cgcgctcaag cgttttcgtc
     3121 ccgttatttg ccgttgagca ggctgcgacc acgaccggaa cctggatgct ggcacggatg
     3181 tccggcgcat gtctggtgcc cttcgttcca cgccgtaagc cagatggcaa agggtatcaa
     3241 ttgattatgc tgccgccaga gtgttctccg ccactggatg atgccgaaac taccgccgcg
     3301 tggatgaaca aagtggtcga aaaatgcatc atgatggcac cagagcagta tatgtggtta
     3361 caccgtcgct ttaaaacacg cccggaaggc gttccttcac gctattaaat ctcccctgcc
     3421 ggatgcttca gaatggcatc cggcattacc acagcaaatc cccctgattt agcgataaaa
     3481 gctctctgga ttgcgccccc tggaagtcgg gcgcataatt agtgtgctta tcttttcttc
     3541 ttatgttcac cgcgcctggc gcaccaacag cggattgcta tgtcaccctg tggaaatgac
     3601 acccctataa actggaaacg aaacctgatc gtcgcctggc taggctgttt tcttaccggt
     3661 gccgccttca gtctggtaat gcccttctta cccctctacg ttgagcagct tggcgttacc
     3721 ggtcactccg ccctgaatat gtggtccggt attgtcttca gtataatacc ttcctaaata
     3781 cactacctga gagaagagat gaaaagagct atcagctaca tgaggttttc aactgagaag
     3841 cagataaaag gggactctat acgaagacag tctaagctgg tcactgactg gttatcaaag
     3901 aaccctga
//
LOCUS       DADMKF010000248         3903 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.252, whole genome shotgun sequence.
ACCESSION   DADMKF010000248 DADMKF010000000
VERSION     DADMKF010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3903
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.252"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(<1..1363)
                     /locus_tag="JNC24_003970"
     CDS             complement(<1..1363)
                     /locus_tag="JNC24_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HAY9160587.1"
                     /translation="MNRTSPHYCRRSVLSLLISALIYAPPVMAAFTTNVIGVVNDETV
                     DGNQRVDERGTTNNTHIINHGQQSVYGGVSNGSLIESGGYQDVGRNNNYMGQSNNTTI
                     NGGRQTIHDGGISTGTIIDSGNQDVYTGGISNGTTIKGGNSHISGGTANGTIIDGGGQ
                     TVTTQGHVDGTTINKSGYQDITQGSMATNTIINGGRQYVEQSTVGTTTIKNGGEQRVY
                     ESHALDTTIEGGTQSLNNKSTAKNTQIYSGGTQIVDYTSSSDVIEVYSGGVLDVSGGT
                     ATNVTQHDGAILKTNTNGTTVSGTNSEGAFSIHNHVADNVLLENGGHLDINAYGSANK
                     TIIKDKGTMSVLTNAKADATRIDNGGVMDVTRNATNTIINGGTQNINNHGIATGTNIN
                     SGTQNIKSGGKADTTNISTGSRQVVEKDGTATGSNISAGGSLIVYTGGIAHGVNQETG
                     SALV"
     gene            complement(1428..1547)
                     /locus_tag="JNC24_003971"
     CDS             complement(1428..1547)
                     /locus_tag="JNC24_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAY9160588.1"
                     /translation="MPLSYVEHHHFIMMSLMSGEHNTLALSLQDEPPGLVPAG"
     gene            complement(1702..1920)
                     /locus_tag="JNC24_003972"
     CDS             complement(1702..1920)
                     /locus_tag="JNC24_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000340076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAY9160589.1"
                     /translation="MKKLLRTLSAVDARVVCKPHRLGRSMWHLVVLLEELCKRGINFR
                     ALAQSIFAQQWGDECCKSKRICDLKVIV"
     gene            complement(2274..3752)
                     /locus_tag="JNC24_003973"
     CDS             complement(2274..3752)
                     /locus_tag="JNC24_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9160590.1"
                     /translation="MLVSKSNEFNTSSFIDSGNCNERKSSESMELLAYSIINLICKEA
                     ASGTYRGALETLQKMMSECIYHEGNAFVIMGSGEQLKRIKYDVDENNLKVFNVYFDNN
                     EELVTDGEPDVVCLSKQVWEDLLIKLKLENKENAVSETDLSSNKNNVDQFIECAKRNE
                     QTLFDNIRKSDFHVDSFKPGSTRSVILETQPNVCMEPHNLYDNQIDKVSPVTKNSQQV
                     KGHYGDKFKEMQMFLNQMSNALQQTPSLSESKEHTIDIQKITDAFVKEFRGVLIDKNG
                     NCSERLFNFYECCYKFLPRAQPQDKIESYNSALQAFSIFRSSTLNNNDVGFNFKLFPE
                     VKLSGENLETVFKYKKGSFVREIARINITLQKEEDGLYNLGGLDFKGCFFSGQNFSNY
                     DIQYVNWRTSLFDLDTPCIFNAPAYNKSNEKSLKPVSENGLSGVLTDRNNKIKLITGV
                     APFDDILFMDDDFDDSSSEDDPVENSPVVTSPVVSSSKSSFQ"
BASE COUNT         1093 a          864 c          680 g         1266 t
ORIGIN      
        1 caactaaagc actgcccgtc tcctggttaa ccccatgtgc tataccgccg gtatagacaa
       61 tcagcgagcc tccggcgcta atattgctgc cagttgccgt accatctttc tcaacaacct
      121 gccggctccc ggtggaaata tttgtcgtgt cagctttccc gccgctcttg atattttgtg
      181 ttccgctgtt gatattggta cccgtggcaa taccatgatt attaatattc tgtgtaccac
      241 cattgattat ggtattggtc gcgtttctgg taacatccat taccccgccg ttatctattc
      301 gggtcgcatc agctttagca ttcgttaaaa ctgacattgt tcctttatct ttaataatcg
      361 tcttatttgc cgaaccatat gcgtttatgt ctaaatgacc accgttttcc agcaacacat
      421 tgtctgccac gtgattgtgg atggagaatg caccttcact attcgtaccg ctcaccgtcg
      481 taccgttagt gtttgttttt aaaattgcac catcgtgctg ggtaacattt gttgccgtac
      541 caccactaac atcaagcacg ccaccggaat aaacttcaat aacatccgag gagctggtgt
      601 aatcaacaat ttgcgtacca ccagaataga tctgagtatt ttttgccgtt gacttattat
      661 tcagagactg agttccacct tcaatcgtcg tgtccagcgc atggctttca tatactcttt
      721 gctcaccgcc atttttaatg gtggttgttc ctactgtgct ctgttcaaca tactgtcgtc
      781 caccatttat gattgtgttc gttgccatac ttccttgcgt gatgtcctga taaccggact
      841 tatttatcgt tgtaccatca acatgcccct gagttgttac tgtctgccct ccaccatcaa
      901 tgatggttcc attcgcagtc cccccactta tgtgtgaatt accaccctta attgtcgttc
      961 cattgctgat accgcctgta taaacgtcct gattgccact gtcgattatc gtaccggtgg
     1021 aaatgccccc gtcatgaatt gtctgtcttc ctccgttaat ggttgtatta ttagactgcc
     1081 ccatataatt gttattcctt ccaacatctt gatatccacc agattcaata agacttccat
     1141 tagatactcc gccataaaca ctctgctggc catggttgat aatatgagtg ttatttgttg
     1201 tacctcgttc atctactcgt tggttgccat ctacagtctc atcgtttacc acaccaataa
     1261 cattagtagt aaaggccgcc atcacgggcg gggcatatat caaggcagat atcaataagg
     1321 aaagtactga gcggcgacaa taatggggac tggtcctgtt cataaatttc atcctctgaa
     1381 aagtgaatac tgagtagcgt ttaagcgact ttagctctgc tgcaacatca gcccgcaggc
     1441 accagaccgg ggggttcatc ctgaagagac agcgcaagtg tattgtgttc accgctcatc
     1501 aaagacatca tgatgaaatg atgatgttcc acataagaaa gaggcatttt ttaaacacag
     1561 tgcgctgaag tgtgattgga taaaaaagtc aatctattca ggaaatacgg gcgtattctt
     1621 ttctttcgac aatcaggccg tcggcaaaat aaaatgattt acataatcgt ttctgatgaa
     1681 tatcttctgc tcacataaaa atcacacaat aactttgaga tcgcagattc ttttactttt
     1741 acagcattcg tccccccatt gttgggcaaa tatagattgg gccagagcac gaaagttaat
     1801 accacgtttg cacagctcct ccaacagcac gacaagatgc cacatactgc gccccagtcg
     1861 gtgcggttta cagactaccc gtgcgtccac cgccgataat gtcctgagca gttttttcag
     1921 tccggacctc ctgccacggt tttacgttga agaggtaacc ctgagcacgc agttcttcag
     1981 tcaggcgtgg tgcaccgtaa cgctgttatt gctgggtaag atcaaaaaac tttcaggcag
     2041 ctaaggaaag ttgaaccaga cattagatga aatatttcaa ccaattacag caccaattta
     2101 aactcagtaa atcacagcac agatcaaggg gttacgtgaa agcgtagccc ctttttcttt
     2161 ggtagtggca gcaaaatggt tgtagtgtaa aaaataatcc cgtttaatca atcaataata
     2221 catattgttt caatctacgt tattatctct ttgtaaaaat agccatttat taatcattga
     2281 aaactgcttt tagaacttga tacaacggga ctagtcacaa caggactatt ctcaacggga
     2341 tcatcctcag aggaactatc atcaaagtca tcatccataa ataaaatatc atcgaatggt
     2401 gccacgcccg tgatgagttt tattttatta ttacgatcag tcaagactcc acttaaaccg
     2461 ttttcgctca caggttttaa tgatttttca ttactcttgt tgtaagcagg cgcattaaaa
     2521 atacacggag tatcaagatc aaacaatgac gttctccagt tcacatattg aatatcatag
     2581 ttactgaagt tctgtccaga aaagaagcat cccttaaaat ccaatccacc taaattatat
     2641 aaaccatcct cttctttttg gagagtaatg ttaattctgg ctatctcccg gacaaaactt
     2701 ccttttttgt atttgaatac cgtttcaaga ttttcaccag acagcttgac ttctgggaat
     2761 aatttaaaat taaaccctac atcattatta ttcaaagtag atgaacgaaa gatggaaaaa
     2821 gcttgtagtg ctgaattata gctttcgatt ttatcttgag gctgcgctct tggtaaaaac
     2881 ttatagcagc actcataaaa attgaataaa cgttctgaac aattcccatt tttatcaatt
     2941 aatacccccc ggaattcttt cacaaaggca tccgttattt tttgaatatc tatggtgtgt
     3001 tcctttgact ctgacaaaga tggagtctgt tgaagtgcat tgctcatctg gttaaggaac
     3061 atctgcattt ctttaaattt atctccatag tggcctttaa cttgctgaga gtttttagtt
     3121 acaggtgaaa ccttatctat ttggttgtca tataaattat gtggttccat acagacattt
     3181 ggttgtgttt ctaaaataac actacgtgta ctacctggct taaatgaatc aacatgaaaa
     3241 tcactttttc ttatattatc gaatagggtc tgttcatttc tcttagcgca ttcaataaac
     3301 tgatcaacat tatttttatt cgatgacagg tcagtttcag aaaccgcatt ttctttgttc
     3361 tccagcttta gtttaatgag aagatcttcc cagacctgct tgcttaaaca tactacgtca
     3421 ggctcaccat cagtaactaa ctcttcatta ttatcaaagt atacgttgaa tacttttaag
     3481 ttattttcat caacatcata tttaatacgt tttaattgct ctccagatcc cataatgaca
     3541 aaggcgttgc cttcatgata tatacattca gacatcattt tttgtaaagt ttcaagagca
     3601 ccgcgatacg tccctgaggc agcttcctta caaattaaat ttataatact ataagctagt
     3661 agctccatgg attcagaaga ttttctttca ttacaatttc cactatctat aaatgagcta
     3721 gtgttaaatt cgttgctttt actaactaac attgtctatt cctcagttaa tgtctacatg
     3781 gctattttta attttattat tgtttgtcac tataaaaaat cgctcatttg agacaattgc
     3841 tggcattaac agcttcatat gctatacatg gtacttttaa ttaaattagc acaagaatgt
     3901 taa
//
LOCUS       DADMKF010000249         3866 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.253, whole genome shotgun sequence.
ACCESSION   DADMKF010000249 DADMKF010000000
VERSION     DADMKF010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3866
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.253"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            752..1468
                     /gene="nanC"
                     /locus_tag="JNC24_003974"
     CDS             752..1468
                     /gene="nanC"
                     /locus_tag="JNC24_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HAY9160591.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            1488..2594
                     /locus_tag="JNC24_003975"
     CDS             1488..2594
                     /locus_tag="JNC24_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HAY9160592.1"
                     /translation="MNKTIMALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPRDQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLMSLAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLDPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            2659..3639
                     /locus_tag="JNC24_003976"
     CDS             2659..3639
                     /locus_tag="JNC24_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709926.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HAY9160593.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHSRIKQL
                     ARFAHTHPGGPPCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPNNAGILIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPQNKFLGVCWMQGEFDLMTCDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHSYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSTAARRGIISDRFVEAILQFWRER"
BASE COUNT         1162 a          801 c          877 g         1026 t
ORIGIN      
        1 gacttcagaa gcgaccagtt acaagccaca aatcaggaaa catatttcat ggtgagaatt
       61 atgatcagcc tgatggcggg taaacacttg ttttcaccac gctcactgag ttagcttgca
      121 aagcttccct tgtttagacc ctaaataaat tttttataaa acaaacagtt aaaagaaatc
      181 ctttagtttt tgaagaccac ctcaagtgtt cgtctggctt cacatggcat cttcctctta
      241 gaaaaagatc gacatatttt gtgacacgaa ttgcaaatct cgttttgttg tatggcttgc
      301 gtgatttttg atctggtata acaggtataa aggtgcaccg cgatagtcaa tgagacaggg
      361 catctcgcaa tctatggcaa acatcacttc agttctttct catcgggtga tgaaaacgca
      421 cttcagtctg aaaggaatat gaaaatgaga tcaacagaca ctctatttta tgactctggg
      481 taaaatggat tgagtaagtg atatagctta cgaacattca aatcaattaa acatcagaag
      541 agattttata ctcaggtatt taatctgatt ctctgtttat ttaaataatg tggaaagaga
      601 attttcacag gagaccttat acaaaaatat aaaataagct acccgttgcc aaagacacta
      661 taagcctggc aaaaaatatt acacaacata aatgctaatt gtttatgcgg gctttgtatt
      721 gctttctgta tcctacaaat gagtgaaatt tatgaaaaag gctaaaatac tttctggcgt
      781 attattactg tgcttttcgt caccattaat ttctcaggct gcgacactgg acgtacgtgg
      841 tggatatcgt agcggaagcc atgcctatga gactcgactc aaagtcagtg agggatggca
      901 aaatggatgg tgggcaagca tggaaagtaa tacctggaat accattcatg ataataaaaa
      961 ggaaaatgcc gcactcaatg atgttcaggt tgaagttaat tacgcgatta aacttgatga
     1021 tcaatggacg gtgcgcccgg gaatgttaac gcattttagc agcaacggca cacgctacgg
     1081 accctacgta aaactgtcct gggacgcgac aaaagatctt aattttggca ttcgctatcg
     1141 ttacgactgg aaagcttacc gacaacaaga cttatccggt gatatgtctc gtgataacgt
     1201 tcatcgttgg gatggatatg tcacttacca tattaatagt gatttcacct tcgcatggca
     1261 aacgacgcta tacagcaaac agaatgatta tcgctatgca aaccacaaga aatgggcgac
     1321 ggaaaatgcc tttgttctac aataccatat gacgcccgat attacgccat acatagaata
     1381 tgactacctt gaccgccagg gtgtttacaa cggcagagat aatttatcgg aaaacagtta
     1441 tcgcattggt gtgtcattta aactgtagta gacaggagac agtcacaatg aataaaacta
     1501 taatggcgct tgctatcatg atggcttcat ttgccgcaaa cgcgtctgta ttaccggaaa
     1561 ctcctgtgcc atttaaaagt ggtaccggag caattgataa cgacactgtc tacattggtt
     1621 taggtagcgc aggtacagca tggtacaagc tagatacaca ggccaaagat aaaaaatgga
     1681 ctgcgttagc tgcattccct ggtggaccga gagatcaagc aacctctgca tttattgatg
     1741 gcaatctata tgtgtttggc ggcattggca aaaacagcga gggattgact caggtattta
     1801 atgacgtaca caaatacaac cccaaaacca atagctgggt taaattgatg tcgctcgcgc
     1861 cgatgggcat ggcgggtcat gtgacttttg tacacaacgg caaggcttat gttactggcg
     1921 gtgttaacca gaatatcttc aatggctatt ttgaagatct caacgaggct ggaaaagatt
     1981 caaccgctat agataaaatc aacgcccact attttgacaa aaaagcagaa gattatttct
     2041 tcaataagtt tctgttgtct tttgatccct caacacagca atggagttac gctggcgaat
     2101 cgccctggta cggaacggct ggtgcggcgg ttgtgaataa aggtgataaa acctggctta
     2161 ttaatggcga agccaaacca ggattgcgaa cggatgccgt atttgaactt gatttcaccg
     2221 gtaataattt aaaatggaat aagcttgatc ccgtctcatc accagatggc gtcgctggcg
     2281 gttttgcggg gataagcaat gattctctta tatttgccgg aggggccgga ttcaaaggtt
     2341 cacgagaaaa ttaccagaac ggtaagaact atgcgcatga aggcctgaaa aaatcatata
     2401 gcactgatat tcatctttgg cataacggga aatgggataa atcgggtgaa ttatcgcaag
     2461 gtcgggccta cggagtatca ttgccctgga ataatagttt attaattatt ggcggtgaaa
     2521 ctgcaggcgg caaagcggtg acggattcag ttttaatttc tgtgaaggat aataaagtca
     2581 cagtacaaaa ctaacgcttc agggccccgg taaaggggcc ctgctatcaa tttgtcattt
     2641 gtattacgcg aaaattatat gaacgcaata atatcgcccg attattacta tgttcttacc
     2701 gttgctggtc agtctaatgc catggcgtat ggcgaaggac tgccattacc ggacagggaa
     2761 gatgcgcctc attccagaat taaacaatta gcgagatttg cgcatacgca tcccggaggc
     2821 ccgccatgtc actttaacga cattattcca ctgactcact gcccacacga tgttcaggat
     2881 atgcagggtt atcaccatcc tctggcaacg aatcatcaaa cacagtacgg caccgttggc
     2941 caggcactgc atattgcacg gaaattactg cccttcattc ctaataatgc agggattctt
     3001 atcgttccgt gttgccgtgg cggatcggct tttaccgcgg gcagcgaagg gacatattca
     3061 gaacggcacg gagccagcca tgatgcttgt cgttggggaa cggatactcc gctgtaccag
     3121 gatttagtca gcagaacgcg agccgcactg gcaaaaaatc cgcagaacaa attcctcggc
     3181 gtatgctgga tgcaaggcga atttgactta atgacctgtg actacgcgtc acaccctcaa
     3241 cactttaatc atatggttga agcctttcgt agggatctaa aacaatacca ttctcagctt
     3301 aataatatta ctgacgcgcc atggttttgc ggcgatacca cctggtactg gaaagaaaat
     3361 ttcccgcatt cgtatgaagc tatttatggc aattatcaaa ataatgtttt agccaatatt
     3421 attttcgtcg acttccagca acaaggtgaa agaggactga cgaacgcgcc tgatgaagac
     3481 ccggacgatt taagcacggg atattacggt tcagcgtacc ggtcaccgga gaactggacg
     3541 acggcactgc gaagcagtca tttcagcacg gcagcccgtc gggggattat ttctgacaga
     3601 tttgtagaag caattttgca gttttggcgc gaaaggtgag cggggagttt attcatgggg
     3661 gatgtccttg ttcgttctca gagtagacaa caaaaaacaa ataagatcaa ggtaatatct
     3721 cttcacttgc gggctcgaac agcaggcaat tgccaatata ataatctggc acaagggtga
     3781 aaaacgcgca ccgcataaac cgttgttatt gcttacagat tagccggatg ccttaataga
     3841 catccgcgcc ttttcgatta tggctc
//
LOCUS       DADMKF010000250         3864 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.254, whole genome shotgun sequence.
ACCESSION   DADMKF010000250 DADMKF010000000
VERSION     DADMKF010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3864
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.254"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            1..1803
                     /gene="fimD"
                     /locus_tag="JNC24_003977"
                     /pseudo
     CDS             1..1803
                     /gene="fimD"
                     /locus_tag="JNC24_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308621.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="outer membrane usher protein FimD"
     gene            1816..2823
                     /gene="fimH"
                     /locus_tag="JNC24_003978"
     CDS             1816..2823
                     /gene="fimH"
                     /locus_tag="JNC24_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrin D-mannose specific adhesin FimH"
                     /protein_id="HAY9160594.1"
                     /translation="MKIICRLLLAMACLCLANISWATVCANSTGVAEDEHYDLSNVFN
                     STNNQPGQIVVLPEKSGWVGVSAICPLGTLVNYTYRSYVTNFIVQETIDNYKYMQLND
                     YLLGAMSLVDSVMDIQFPPQNYIRMGTDPNVSQNLPFGVMDSRLIFRLKVIRPFINMV
                     EIPRQVMFTVYVTSTPYDPLVTPVYTISFGGRVEVPQNCELNAGQIVEFDFGDIGASL
                     FSAAGPGNRPAGVMPQTKSIAIKCTNVAAQAYLTMRLEASAVSGQAMVSDNQDLGFIV
                     ADQNDTPITPNDLNSVIPFRLDAAAAANVTLRAWPISITGQKPTEGPFSALGYLRVDY
                     Q"
     gene            2834..3349
                     /gene="sfmF"
                     /locus_tag="JNC24_003979"
     CDS             2834..3349
                     /gene="sfmF"
                     /locus_tag="JNC24_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria assembly protein"
                     /protein_id="HAY9160595.1"
                     /translation="MRRVLFSCFCGLLWSSSGWAADPLGTININLHGNVVDFSCTVNT
                     ADIDKTVDLGRWPTTQLLNAGDTTALVPFSLRLEGCPPGSVAILFTGTPASDTNLLAL
                     DDPAMAQTVAIELRNSDRSRLALGEASPTEEVDANGNVTLNFFANYRALASGVQPGVE
                     KADAIFMINYN"
     gene            complement(3352..>3864)
                     /gene="fimZ"
                     /locus_tag="JNC24_003980"
     CDS             complement(3352..>3864)
                     /gene="fimZ"
                     /locus_tag="JNC24_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria biosynthesis transcriptional regulator
                     FimZ"
                     /protein_id="HAY9160596.1"
                     /translation="TIDYLRTRPVDLIIMDIDLPGTDGFTFLKRIKQIQSTVKVLFLS
                     SKSECFYAGRAIQAGANGFVSKCNDQNDIFHPVQMILSGYTFFPSETLNYIKSNKCST
                     NSSTVTVLSNREVTILRYLVSGLSNKEIADKLLLSNKTVSAHKSNIYGKLGLHSIVEL
                     IDYAKLYELI"
BASE COUNT          988 a          846 c          959 g         1071 t
ORIGIN      
        1 tggtgtacgg ctttattctt ccgataatat gtatccagat agccagcaag ggtttgcccc
       61 aacggtacgt gggattgccc gtacggcggc ccagctaacg attcggcaaa atggttttat
      121 tatctatcaa agctatgttt cccccggtgc ttttgaaatt actgatttgc acccgacatc
      181 ttcaaatggc gatctggatg tcaccatcga cgagcgcgat ggcagaatta cacaattccg
      241 tattcaacag tgccgatttt acaacgcgaa gggcgtttca aatttgacct gacggcgggc
      301 gattttcgta gcggtaatag tcagcaatca tcacctttct tttttcaggg cacggcactc
      361 ggtggtttac cacaggaatt tactgcctac ggcgggacgc aattatctgc caattacacc
      421 gcctttttat tagggctggg gcgcaacctc gggaactggg gcgcagtgtc gctggatgtg
      481 acccatgcgc gcagccagtt agccgacgac agtcgtcatg agggggattc cattcgcttc
      541 ctctatgcga aatcgatgta caccttcggc accaattttc agttaatggg ttaccgctat
      601 tcgacacaag gtttttatac ccttgatgat gttgcgtatc gtcgaatgga ggggtacgaa
      661 tatgattacg attacgacgg tgagcatcgc gatgaaccga taatcgtgaa ttaccacaat
      721 ttacgcttta gccgcaaaga ccgtttgcag ttaaatattt cacaatcact taatgacttt
      781 ggctcgcttt atatctctgg tacccatcaa aaatactgga atacttcgga ttcagatacc
      841 tggtatcagg tggggtatac cagcagctgg gttggcatca gttattcact ctcattttcg
      901 tggaatgaat ctgtagggat ccccgataac gaacatattg tcggacttaa tgtttcagtg
      961 cctttcaatg ttttgaccaa acgtcgctac acccgggaaa atgcgctcga ccgcgcttat
     1021 gcctccttga acgctaaccg taacagcaac gggcaaaata gctggctggc aggcgttggt
     1081 gggaccttac tggaaggcca caacctgagt tatcacgtaa gccagggcga tacctcgaat
     1141 aatgggtaca cgggcagcgc cacggcaaac tggcaggcca cttacggtac gctgggggtc
     1201 gggtataact acgaccgcga tcaacatgac gttaactggc agctgtctgg cggtgtggtc
     1261 gggcatgaaa atggcataac actgagccag cctttagggg ataccaatgt tttgattaaa
     1321 gcgcttggcg caggcggtgt acgcattgaa aatcaaactg gcattttaac cgactggcgc
     1381 ggctatgcgg tgatgccgta tgccacggtt tatcggtata accgtatcgc gcttgatacc
     1441 aatacgatgg ggaattccat cgatgttgaa aaaaatatta gcagcgttgt gccgacgcaa
     1501 ggcgcgttgg ttcgtgccaa ttttgatacc cgcataggcg tgcgggcgct cattaccgtt
     1561 acccagggcg gaaaaccggt gccgtttgga tcactggtac gggaaaacag taccggaata
     1621 accagtatgg tgggtgatga cgggcaagtt tatttaagtg gtgcgccatt gtctggtgaa
     1681 ttactggttc agtggggaga cggcgcgaac tcacgctgca ttgcgcacta tgtattgcca
     1741 aagcaaagct tacagcaagc cgtcactgtt atttcggcag tttgcacaca tcctcgctca
     1801 taaaggaaat tatcaatgaa gataatctgc agattattat tggcaatggc atgtttgtgt
     1861 ctggcaaaca tatcctgggc tactgtttgt gcaaatagta ctggcgtagc agaagatgaa
     1921 cactatgatc tctcaaatgt ctttaatagc accaataacc agccagggca gattgttgtt
     1981 ttaccggaaa aatccggctg ggtaggtgtc tcagcaattt gtccactcgg cacgctggtg
     2041 aattatacat accgtagtta tgtcactaac tttattgttc aggaaactat cgataattat
     2101 aaatatatgc aattaaatga ttatctatta ggtgcgatga gtctggttga tagtgtgatg
     2161 gatattcagt tccccccgca aaattatatt cggatgggaa cagatcctaa cgtttcgcaa
     2221 aaccttccgt tcggggtgat ggattctcgt ttgatatttc gtttaaaggt tattcgtccc
     2281 tttattaaca tggtggagat ccccagacag gtgatgttta ccgtgtatgt gacatcaacg
     2341 ccttacgatc cgttggttac acctgtttat accattagtt ttggtggccg ggttgaagtg
     2401 ccgcaaaact gcgaattaaa tgccgggcag attgttgaat ttgattttgg tgatatcggc
     2461 gcatcgttat ttagtgcggc agggccgggg aatcgaccag ctggcgtcat gccgcaaacc
     2521 aagagcattg cgatcaaatg tacgaatgtt gctgcgcagg cttatttaac aatgcgtctg
     2581 gaagccagtg ccgtttctgg tcaggcgatg gtgtcggaca atcaggattt aggttttatt
     2641 gtcgccgatc agaacgatac gccgatcacg cctaacgatc tcaatagcgt tattcctttc
     2701 cgtctggatg cagctgcggc agccaatgtc acacttcgcg cctggcctat cagtattacc
     2761 ggtcaaaaac cgaccgaagg gccgtttagc gcgctggggt atttacgcgt cgattatcaa
     2821 tgaggtacgg agaatgagaa gagtactctt tagctgtttc tgcgggttac tgtggagttc
     2881 cagtggatgg gcagctgacc ctttgggaac gattaatatc aatttgcacg gtaacgttgt
     2941 tgatttctcc tgtaccgtaa acactgcgga tattgataag acggtagatt taggcagatg
     3001 gcctacgaca caattactga acgctggcga taccacggca ctcgtccctt ttagcctgcg
     3061 gctggaggga tgtcctccgg gttccgttgc gattttattt acgggaacgc cggcatccga
     3121 taccaacctg ctggctctgg atgatcccgc aatggcacaa accgtcgcca tcgaattacg
     3181 taatagcgat cgctcccggc tcgcactggg ggaggcgagc ccgactgagg aagtagatgc
     3241 aaatggcaat gtcacactaa acttttttgc caattatcga gcgttagcca gcggtgttca
     3301 gccaggtgtg gagaaagcgg atgcgatatt tatgatcaat tataattaat attatattaa
     3361 ttcgtataat ttggcgtagt cgataagctc tacaattgaa tgcaaaccta gtttgccata
     3421 aatattagat ttatgcgcac taactgtttt attgctaagt aataacttat cggcaatttc
     3481 tttattagat aatccgctaa ccagataacg taatatggtc acttcacgat tagatagcac
     3541 agtgaccgtt gaactattcg tactacattt attgcttttt atatagttga gcgtttcgct
     3601 aggaaaaaac gtgtatccgg agaggatcat ctgaacggga tgaaaaatat cattctgatc
     3661 attgcattta ctgacaaaac cgttagcacc agcttgtatc gctctgccag cataaaagca
     3721 ttctgatttc gatgataaaa ataacacttt cactgtgctc tggatttgtt tgatcctttt
     3781 caggaaggta aaaccgtctg ttccgggcaa gtctatatcc ataatgatta aatcaacagg
     3841 acgggttcgg agataatcga tggt
//
LOCUS       DADMKF010000251         3853 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.255, whole genome shotgun sequence.
ACCESSION   DADMKF010000251 DADMKF010000000
VERSION     DADMKF010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3853
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.255"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            115..510
                     /gene="caiF"
                     /locus_tag="JNC24_003981"
     CDS             115..510
                     /gene="caiF"
                     /locus_tag="JNC24_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HAY9160597.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVAEISCAVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSRDKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(719..1309)
                     /gene="caiE"
                     /locus_tag="JNC24_003982"
     CDS             complement(719..1309)
                     /gene="caiE"
                     /locus_tag="JNC24_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HAY9160598.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFHGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(1315..2100)
                     /gene="caiD"
                     /locus_tag="JNC24_003983"
     CDS             complement(1315..2100)
                     /gene="caiD"
                     /locus_tag="JNC24_003983"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HAY9160599.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFDGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPAIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIFRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            complement(2209..3762)
                     /gene="caiC"
                     /locus_tag="JNC24_003984"
     CDS             complement(2209..3762)
                     /gene="caiC"
                     /locus_tag="JNC24_003984"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HAY9160600.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTTNLFYTLGIRKGDKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESA
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYL
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQVQKYRATVTECIPMMIRTL
                     MAQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPG
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYCDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
BASE COUNT          909 a         1047 c          941 g          956 t
ORIGIN      
        1 ttatcgacgg tgaagttgca tactatcgat atatccacaa ttttaatatg gccttgttta
       61 attgcttcaa aacgagtcat agccagactt ttaatttgtg aaactggagt tcgtatgtgt
      121 gaaggatatg ttgaaaaacc actctacttg ttaatcgccg aatggatgat ggctgaaaat
      181 cggtgggtga tagcaagaga gatctctatt catttcgata ttgaacacag caaggcggtt
      241 aataccctga cttatattct gtcggaagtc gcagaaataa gctgcgcagt taagatgatc
      301 cctaataagc tggaaggacg gggatgccag tgccagcgac tggtaaaagt ggtcgatatc
      361 gatgagcaaa tttacgcgcg cctgcgcaat aacagccggg ataaattagt cggcgtaaga
      421 aagacgccgc gtattcctgc cgttccgctc acggaactta accgcgagca gaagtggcag
      481 atgatgttgt caaagagtat gcgtcgttaa tgtcatttcg ttgataccac gcagcctggt
      541 tgctggatgc aatgctggcg catcttatcc agcctacggt tgctctccag ccgggttgga
      601 taaggcgttt atgccgcatc cgacagctat cgccggatgc gatgctggcg catcttatcc
      661 ggcctacggt tgctctccag cagagttgga taaggcgttt acgccgcatc cggcaagatc
      721 accgtttagg cgtcacatcc gtcgtcccct gcaaacgggg gcgattttcc tccatttgcc
      781 tcagtggctg tgtttcatgt aacgatgcat gacagcgccc gacaagatcc tgatactctt
      841 tggtattcaa ccgtttccag tgtaactcgt cgtcactaac actgcgtaca gcgcgggctg
      901 gcgtacccat caacaactgg cgtttctcgc cgtgaaagcc tgctttgaca aagctcatgg
      961 cggcaacaat gctctcttcg ccaatgaccg cgccatccat aatcacgctg ttcatcccga
     1021 ccaatgcatc gcgaccaatc acacaaccat gcaggatcgc tccgtgcccg atatggccgt
     1081 tttccccaac gatggtgtca gtgtcgcagt agccatgcat aatgcagcca tcctgaatat
     1141 tggctcccgc ttgcacgatc aaccgcccgt agtcaccacg cagtgaggcg agtgggccga
     1201 tgtagacacc ggctcccaca atcacatcgc caatcaagac ggcactggga tggacaaacg
     1261 ccgtcgggtg aaccaccgga attaacccct caaaggcgta atagctcacg gttgttaacg
     1321 tcctttccac accggatcgc gcttctcggc aaacgccagc ggcccttcaa tggcatcctc
     1381 cgaatgcaga accgacggat agtgtttcaa cacgccgctg cgaatatagc gatacgcttc
     1441 ttctaccggc atttcgctgg tggtgcggaa gatctctttc agcgccgcaa tcgccagcgg
     1501 ggcgctgtta accagctgct gcgccagttc gcgggcgtta tccatcagtt ccgcctggct
     1561 aaccacgcgg ttgactatcc cccaacgcag cgcctcttct gcgcccattc gtctgccggt
     1621 catcaccatt tcattgacga tggcaggcgg caggatcttc ggtagacgca gcacgccgcc
     1681 gctgtcagga acgatgccca gcttggcttc cggcagggcg aagctggcgt tatcggcaca
     1741 aacaataaaa tctgccgcca gcgccagttc gaaaccgccg tcaaaggcat agccgttcac
     1801 agctgcgata accggtttgt cgagattgaa aatttcggtt aatcccgcaa atccacccgg
     1861 gccaaagtca gcatccggtg cttcgccttc tgctgccgct tttaaatccc agcccgcgga
     1921 aaagaacttc tctccggcac cggtaataat ggcgacacgt aattgcggat cgtcacggaa
     1981 atttagaaat acttcgccca tttcaaagct ggtttttgca tcaatagcat tcgcttttgg
     2041 acgatcaagg gtaatttcca gaattgatcc attgcgggtc agatgtaatg attcactcat
     2101 tccttttctc catttttgct ttttcaggga cgacaacatc cctgcaaaaa atgcatattg
     2161 ttttatagcg tgattattag ttggcagggt agttccctgc tgtttcattt atttcagatt
     2221 ctttctaatt attttccccg agcaattacg tggcagatct tttctgatct ccagataaga
     2281 gggcacttta aatttcgcca tattttgttc gcagaagcgg aaaaattcct cttcgctcaa
     2341 tgtttcacct tcattcagca ccacaaatgc tttgatggct tcatcgcgaa tcgaatcttt
     2401 aatacccaca accacgatat cctgaatttt tggatgggtg gcgatgatat tttccagctc
     2461 cacgcaggag acattctcgc caccgcgttt gatcatattg cagcggcgat cgacgaaata
     2521 aaaaaagccc tcttcgtcgc agtatccggt atcgccggta tgtagccagc cgtcggcttc
     2581 cagcactttc gcagtggctt tcgggttgag aaaatactct ttgaagatgg ttttccctgg
     2641 tacgccttta atacagattt caccgatctc acctgccggg agcgggcgat tgtgatcgtc
     2701 gcggatctcc gcttcgtagc aaaaccccgc ccgaccaatc gacggccagc gtcgtttatc
     2761 gccagggcga tcgccgataa tgcccacaat ggtttccgtc atcccataag acgtcagcaa
     2821 gcgaacgccg aagcgttcac aaaacgcatc tttttcctgc tccgacaagt tgagataaaa
     2881 catcacttcc cgcaggcggt gttgccgatc gttcgctgaa ggcggctgcg ccatcaaggt
     2941 acggatcatc atcggaatac attcggtaac ggtggcgcgg tacttctgta cctgtcccca
     3001 gaaggcgcgg gcgctgtatt tctcgaccag cacaaaggtg gccccggcag aaaacgccgc
     3061 catcgccgca gtacactggc aatcgatatg aaacgcaggc attaccgtca ggtagacgtc
     3121 atcgtcacgc agtgcacact gccaggcgga gtaatatcca gcgaagcgca ggttgtaatg
     3181 ggtaatcacc acacctttcg gtcgggaggt ggtgccggag gtgaagagaa tttccgccgt
     3241 atcgtcagtc gatagcggcg gtgcatagca caaggtggca ggttgttgat ttttcagttg
     3301 agtaaacgaa ctcacgccat catcagcggg aagtgccaca tctgtcaggc aaatgtgccg
     3361 caattgagtg gcatcttcct gctgaatctg ttgatacata ggatagaatt gcgcactggt
     3421 caccagcagg cacgcctggc tattttgcag gatccacgcg ctttcctcgc gcaacaggcg
     3481 ggcgttaatc ggcaccataa tcgcgccaat ttttgccagc ccgaaccagc aaaagataaa
     3541 ttccgggcag ttgtcgagat gtagagcaac cttgtcgcct ttgcgaatcc ccagcgtata
     3601 aaacaggttt gtcgtgcggt taatctcctg atttaactca agataactat accggttaac
     3661 gactccgccg ctggattcac aaatcagcgc cgttttatga ccgtaaacgt ccgccagatc
     3721 gtcccacatt tgacgtagat gttgtccgcc aatgatatcc attgcacctc tatccatttt
     3781 tgttcgtttg ttattgggcg ggcgctagtc aggcaagccg actgacgcca cgcgtttagt
     3841 cctcaacttt ggc
//
LOCUS       DADMKF010000252         3833 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.256, whole genome shotgun sequence.
ACCESSION   DADMKF010000252 DADMKF010000000
VERSION     DADMKF010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3833
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.256"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(<1..266)
                     /locus_tag="JNC24_003985"
     CDS             complement(<1..266)
                     /locus_tag="JNC24_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9160601.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRELTDAGGFLDISL"
     gene            353..760
                     /gene="nudG"
                     /locus_tag="JNC24_003986"
     CDS             353..760
                     /gene="nudG"
                     /locus_tag="JNC24_003986"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HAY9160602.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFADGKVELDES
                     QQQALVRELNEELGIEATVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARAAD"
     gene            complement(726..998)
                     /locus_tag="JNC24_003987"
     CDS             complement(726..998)
                     /locus_tag="JNC24_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HAY9160603.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDQNYSEGAVIKAEGILLQCQRDDKTLSTNPLLWRRVKP"
     gene            1234..2577
                     /gene="gdhA"
                     /locus_tag="JNC24_003988"
     CDS             1234..2577
                     /gene="gdhA"
                     /locus_tag="JNC24_003988"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013366584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HAY9160604.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQIQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGEVMRFCQALMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMRVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKASRDGRVADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDVDAAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLAQGVI"
     gene            complement(2694..3733)
                     /locus_tag="JNC24_003989"
                     /pseudo
     CDS             complement(2694..3733)
                     /locus_tag="JNC24_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310495.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
BASE COUNT          998 a          931 c          917 g          987 t
ORIGIN      
        1 tcaagagaaa tatcaagaaa gccgcccgcg tcggtaagct ctctgcgccg cgccagcgca
       61 ccgtccagac catcaagcag cctgttcaac aaaatgacga ctagcgccgc cagataccag
      121 cccagcgcca gaaacggcag agccagcacg ccgatggcaa atcccactaa tgttaaacca
      181 tctggcgtaa tgcccggttt atcaagaacc cgcacgcact gatgcaacaa cggtttaatc
      241 cggggatgaa gatggcggtc tagcacaggc actccttaaa tataaagcct ttctgattga
      301 gcaacagtgc ggatattatg gcatttttcg cttatctgcc cgtgtgtaat ttatgaaaat
      361 gattgaagtt gttgccgcca tcattgaacg tgatggcaaa attttactcg cgcaacgccc
      421 cgcccagagc gatcaggcgg gattatggga gtttgccgat ggtaaagtcg agctggatga
      481 aagccagcag caggcactgg tgcgtgagtt aaacgaagaa ttgggcatcg aagcaactgt
      541 gggtgagtat gttgccagcc atcagcgaga agtttcgggc cggattatcc atcttcatgc
      601 ctggcacgta cccgacttcc acgggacgtt acaggcacat gaacatcagg cgctggtctg
      661 gtgctcacct gaagaggcgc tgcaatatcc gctggcccct gctgacattc cactattaga
      721 ggcgtttatg gctttacgcg ccgccagagc agcggattag tgctaagggt tttgtcatcc
      781 cgctggcatt gcagcagtat tccttcggct ttaattaccg ccccttcaga ataattttga
      841 tcctgataaa cgcagcactg agtacagggc tgcgctgact gcccgcctga actgaacact
      901 tctggcggta cgtttacctc cacgtccgga cgataatgcg ggttagccag tgcaattaat
      961 ggaaatgcta atactacggc gaacaatgct cgactcacag ggaactcctt aacgttattg
     1021 tctctgcaac tgataacggt agccgggtgg caaaacttta gcgtctgagt tatcgcattt
     1081 ggttatgaga ttactctcgt tattaatttg ctttcccggg tcattttttt cttgcttacc
     1141 gtcacattct tgatggtata gtcgaaaact gcaaaagcac atgacataaa caacataagc
     1201 acaatcgtat taatatataa gggttttata tctatggatc agacatattc tctggagtca
     1261 ttcctcaacc atgtccaaaa gcgcgacccg aatcaaaccg agttcgcgca agccgttcgt
     1321 gaagtaatga ccacactctg gccttttctt gaacaaaatc caaaatatcg ccagatgtca
     1381 ttactggagc gtctggttga accggagcgc gtgatccagt ttcgcgtggt atgggttgat
     1441 gatcgcaacc agatacaggt caaccgtgca tggcgtgtgc agttcagctc tgccatcggc
     1501 ccgtacaaag gcggtatgcg cttccatccg tcagttaacc tttccattct caaattcctc
     1561 ggctttgaac aaaccttcaa aaatgccctg actaccctgc cgatgggcgg tggtaaaggc
     1621 ggcagcgatt tcgatccgaa aggaaaaagc gaaggtgaag tgatgcgttt ttgccaggcg
     1681 ctgatgaccg agctttatcg tcatctgggc gcggataccg acgttccggc aggtgatatc
     1741 ggcgttggtg gtcgtgaagt cggctttatg gcggggatga tgaaaaagct ctccaacaat
     1801 accgcctgcg tcttcaccgg taagggcctt tcatttggcg gtagtcttat tcgcccggaa
     1861 gctaccggct acggtctggt ttatttcaca gaagcaatgt taaaacgcca cggtatgggt
     1921 tttgaaggga tgcgcgtttc cgtttctggc tccggcaacg tcgcccagta cgctatcgaa
     1981 aaagcgatgg aatttggtgc tcgtgtgatc actgcgtcag actccagcgg cactgtagtt
     2041 gatgaaagcg gattcacgaa agagaaactg gcacgtctta tcgaaatcaa agccagccgc
     2101 gatggtcgag tggcagatta cgccaaagaa tttggtctgg tctatctcga aggccaacag
     2161 ccgtggtctg taccggttga tatcgccctg ccttgcgcta cccagaacga actggatgtt
     2221 gacgccgcgc atcagcttat cgccaatggc gttaaagccg tcgctgaagg ggcaaatatg
     2281 ccgaccacca tcgaagcgac tgaactgttc cagcaggcag gcgtactgtt tgcaccgggt
     2341 aaagcggcta atgctggcgg tgtagcaacg tcgggcctgg aaatggcaca aaacgctgcg
     2401 cgcctgggct ggaaagccga gaaagttgac gcacgtttgc atcacatcat gctggatatc
     2461 caccatgcct gtgttgagca tggtggtgaa ggtgagcaaa ccaactacgt gcagggcgcg
     2521 aacattgccg gttttgtgaa ggttgccgat gcgatgctgg cgcagggtgt gatttaagtt
     2581 gtaaatgcct gatggcgcta cgcttatcag gcctacaaat gggcacaatt cattgcagtt
     2641 acgctctaat gtaggccggg caagcgcagc gcccccggca aaatttcagg cgtttatgag
     2701 tatttaacgg atgatgctcc ccagggaaca tttcttatgg gccaacggca tttcttactg
     2761 tagtgctccc aaaattgctt gtcgtaacga taacacgctt caagttcagc atccgttaac
     2821 tttctgcgat agcagcagat atgccagtaa agaaacccca ttagactatt tttttgataa
     2881 tcttcttcgc tttcgaacaa ctcgtgcgcc tttcgagaag caagcattat ataatgccag
     2941 gccagttctt cttcaattgt cccgttttga aaagctgtgc ttgatatcga ggtcatccat
     3001 gataattccg ccgcccatat tagcttcgcc gaggatttac cggagcgatt attagcgcaa
     3061 tcagagatat agtctgaggg aaaaacagca aatttattca acaaggcgat aacctgctct
     3121 ggggcttcct ccatgtttgc tttaaaggta ttggctccat ggtcgccaga aagaaaatgc
     3181 tccattaggc acaataactt tcgctatctt cgatacccca ttgattctct aaagactcgc
     3241 gtcttttact gatgatatcg atcgagtcaa aaggaagcac atgatattgg aaggtatctt
     3301 taccaggttc aggctttcgc ggccagaact ctagcgtttc agaccattgc ttatgataga
     3361 atcgataagg tgcgatcaat tgtagcgcct gtaacttctc gatactgagc ggctcaatac
     3421 ctttagcctg ataataatgc agttgttctt tttttgcttt aaaaccggcc cgaacaataa
     3481 gccccatcat aattaataga taaagaaaag agcatcccgc ggtaatcagg cctctttcat
     3541 tcaaaccgtt ggatgttatc gctgcgaaca caaacattac agcgacaaca catgttaaat
     3601 aaaaccccca cttacaaagc agcatggcct tattttcttt aatcatccgt tcaaaattac
     3661 tattaaatat ttcccagcca ttaaaagaat acttctcgct cccaggatgg ttttgtaata
     3721 aaactttttt catcacattc cctgttatta catactttag aggcgataat tttatccatg
     3781 caaaaaaaat atccaacgaa ttttcttgat ccgctggata tttcagaata tga
//
LOCUS       DADMKF010000253         3797 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.257, whole genome shotgun sequence.
ACCESSION   DADMKF010000253 DADMKF010000000
VERSION     DADMKF010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3797
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.257"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(98..907)
                     /gene="proC"
                     /locus_tag="JNC24_003990"
     CDS             complement(98..907)
                     /gene="proC"
                     /locus_tag="JNC24_003990"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HAY9160605.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            1027..1485
                     /locus_tag="JNC24_003991"
     CDS             1027..1485
                     /locus_tag="JNC24_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HAY9160606.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            1668..2192
                     /gene="aroL"
                     /locus_tag="JNC24_003992"
     CDS             1668..2192
                     /gene="aroL"
                     /locus_tag="JNC24_003992"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HAY9160607.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            2242..2433
                     /gene="yaiA"
                     /locus_tag="JNC24_003993"
     CDS             2242..2433
                     /gene="yaiA"
                     /locus_tag="JNC24_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HAY9160608.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            2691..3368
                     /locus_tag="JNC24_003994"
     CDS             2691..3368
                     /locus_tag="JNC24_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HAY9160609.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLTVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            3440..3724
                     /gene="ppnP"
                     /locus_tag="JNC24_003995"
     CDS             3440..3724
                     /gene="ppnP"
                     /locus_tag="JNC24_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HAY9160610.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
BASE COUNT          951 a          904 c          990 g          952 t
ORIGIN      
        1 cgtgagtggt tgaaatcggc agatttggcg ctttacaaag caaagaaagc cggacgtaac
       61 cgcaccgaag tggcggcctg acgtccggcg aaagtcatca ggatttgctg agtttttctg
      121 atttttccat acacttcgtc atcgcttcga tcactgcggc acggaagcct ttctcttcca
      181 gtacgcgtac cgcttcaatg gtggtgcctc ccggtgagca gaccatatct ttcagtgccc
      241 ccggatgttc tcccgtttcc agcaccattt ttgcggaacc cattaccgcc tgagcggcaa
      301 atttatacgc ctgggcgcgt ggcatcccgc ccagcacggc ggcgtcggcc atcgcttcga
      361 taaacataaa tacgtaggct ggcgaagaac cgctcacacc gaccaccggg tggatcatcg
      421 gctcagcaat tacttccgct tcgccaaagc agcggaaaat attcagcaca tcagcggtat
      481 cttcgggggt taccagcgcg tttggcgtta cggaggtcat cccggcatta accagcgcgg
      541 gagtgttcgg catggcgcgg ataattttcc ggtcatggcc cagcgcgcgg gcaagctggt
      601 cgagcgtgac acctgcagca atagaaacga ccagagagtc tttattcagg ctggaggtga
      661 tttcgctaag cactttaatc atgatgccag gtttaacggc ggcaaaaatg atgtcggcga
      721 tttgcgccac ttcttgcgcc gattctgcgg cgttgatgcc gaactggtca tgcagggcgg
      781 cgactttatc cggggagggg gtgtataccc agatttgccc tggaagcacc tgaccgctgg
      841 caatcagacc gccgagaatg gcttttccca tattgccgca gccaataaaa ccgattttct
      901 tttccattgc ctcactcctg ccgtgaaatt cattgttttg ataatcgctg gcagaagcat
      961 aaacagaact atgccggaag gcaaaagcgc gacacaatag aggattaccc aacaaaggat
     1021 gactttatga caatttgggt ggatgccgac gcgtgtccca atgtaattaa agagattttg
     1081 tatcgcgcgg cggaacgtat gcagatgccg ctggtactgg tagcaaacca gagtttacgc
     1141 gtgccgccat cgcgatttat tcgtacgctg cgcgtcgcgg caggtttcga cgttgccgat
     1201 aacgaaattg tccggcagtg tgaagcgggc gatttggtga tcaccgcaga tatacctttg
     1261 gctgctgaag ccatcgagaa aggcgcagcg gcgcttaatc cgcgcggcga acgttacacg
     1321 ccagcgacca ttcgtgagcg cctgacgatg cgcgatttta tggatacctt acgtgccagt
     1381 gggatccaga ccggcggacc agatagcctt tcacaacgtg accgccaggc ctttgccgcg
     1441 gagctggaga agtggtggct ggaagtgcaa cgtagtcgtg gctaaatgta atttattatt
     1501 tacacttcat tcttgaatat ttattggtat agtaagggat gtattgagat tttcacttta
     1561 agtggaattt tttctttaca atcgaaattg tactagtttg atggtatgat cgctattctc
     1621 atgacaccgg ctttcgccgc attgcgacct attggggaaa acccacgatg acacaacctc
     1681 tttttctgat cgggcctcgg ggctgtggta aaacaacggt cggaatggcc cttgccgatt
     1741 cgcttaaccg tcggtttgtc gataccgatc agtggttgca atcacagctc aatatgacgg
     1801 tcgcggagat cgtcgaaagg gaagagtggg cgggatttcg cgccagagaa acggcggcgc
     1861 tggaagcggt aactgcgcca tccaccgtta tcgctacagg cggcggcatt attctgacgg
     1921 aatttaatcg tcacttcatg caaaataacg ggatcgtggt ttatttgtgt gcgccagtat
     1981 cagtcctggt taaccgactg caagctgcac cggaagaaga tttacggcca accttaacgg
     2041 gaaaaccgct gagcgaagaa gttcaggaag tgctggaaga acgcgatgcg ctatatcgcg
     2101 aagttgcgca tattatcatc gacgcaacaa acgaacccag ccaggtgatt tctgaaattc
     2161 gtagcgccct ggcacagacg atcaattgtt gattttagag cgcctatact taacgttcat
     2221 cccgtgaaat aaggaagaac gatgccaacg aaaccgcctt atcctcgtga agcatatata
     2281 gtgacgattg aaaaaggaaa gccaggacag acggtaacct ggtaccaact cagagccgat
     2341 catcctaaac cagactcgtt gatcagtgaa catccgacgg ctcaggaagc gatggatgcg
     2401 aaaaaacgct atgaggaccc tgacaaagag tgaccgcatc agactgctcg gaagggattc
     2461 tgagtgccac tacaagggat ctgcgtcaca tttttcataa ttcatgtttt tctaataatt
     2521 agaatattaa acaataacaa tccattactg gaatcatttg gaatctttac attatgccgt
     2581 gcacgtctgc tgctacgctt tttgtcattt gtagcacaag taagtgtcag cagtggtgct
     2641 ccacacttgc ccggtaatta acgacgaaag aaaagtaagg tggatgaaca atgagtgcgt
     2701 cgttggcgat cctgaccatc ggcattgtac ctatgcagga ggttttgccg ctcctgacgg
     2761 aatacattga cgaagataat atttcccatc atagcctgct ggggaagtta agtcgtgaag
     2821 aagtgatggc ggagtacgcg ccagaagcag gcgaagacac cattctcaca ttattaaatg
     2881 acaaccagct ggcccatgtt tcgcgtcgta aagtggagcg agacctgcaa ggtgtggttg
     2941 aagtgctcga taatcagggt tatgacgtca ttttattaat gagtacagca aacattagta
     3001 gtatgactgc gcgtaatacg atctttcttg agccgtcgcg aatattgcct ccactggttt
     3061 cctctattgt tgaagatcat caggtggggg ttatcgttcc ggttgaggag atgctgaccg
     3121 ttcaggcgca aaaatggcaa attttgcaga aatcgccggt attttcattg ggtaacccca
     3181 ttcatgattc agaacaaaaa atcattgatg ccgggaaaga attactggca aaaggggctg
     3241 atgtcatcat gctggattgt ttaggatttc accaacgtca tcgcgattta ctgcaaaaac
     3301 agctcgatgt tcctgtcttg ctgtctaacg tattgattgc acggctggct gcggaattac
     3361 tggtgtaatt ttgcgtgaca gccagcgcct ctggccccta tagtgaagta gatgttcaac
     3421 taccagacag ggccagttta tgcttcaaag taatgagtac ttttccggca aagtgaaatc
     3481 aatcggcttt tccagcagca gcactggtcg cgccagcgtg ggtgttatgg ttgaaggcga
     3541 atacaccttc agcaccgctg agccggaaga gatgacggta atcagtggcg cgctgaatgt
     3601 gttactgcct gacgcgaccg actggcaggt gtatgaagcc ggttcggtgt ttaatgttcc
     3661 cggtcacagt gagtttcatc tgcaagttgc cgaacccacc tcttatctgt gccgctatct
     3721 gtaattcctc gccttcccct tgaacgggag ggcatttttc tgaaatatcc tctctttaac
     3781 ccataataat atttcct
//
LOCUS       DADMKF010000254         3735 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.259, whole genome shotgun sequence.
ACCESSION   DADMKF010000254 DADMKF010000000
VERSION     DADMKF010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3735
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.259"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(<1..181)
                     /locus_tag="JNC24_003996"
     CDS             complement(<1..181)
                     /locus_tag="JNC24_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel protein MscS"
                     /protein_id="HAY9160611.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSI"
     gene            425..682
                     /locus_tag="JNC24_003997"
     CDS             425..682
                     /locus_tag="JNC24_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
                     /protein_id="HAY9160612.1"
                     /translation="MIMAKLKSAKGKKFLFGLLAVFIIAASVVTRATIGGVIEQYNIP
                     LSEWTTSMYVIQSSMIFVYSLVFTVLLAIPLGIYFLGGEEQ"
     gene            complement(732..1682)
                     /gene="uspE"
                     /locus_tag="JNC24_003998"
     CDS             complement(732..1682)
                     /gene="uspE"
                     /locus_tag="JNC24_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HAY9160613.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(1834..2586)
                     /locus_tag="JNC24_003999"
     CDS             complement(1834..2586)
                     /locus_tag="JNC24_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FNR family transcription factor"
                     /protein_id="HAY9160614.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            complement(2781..3296)
                     /gene="ogt"
                     /locus_tag="JNC24_004000"
     CDS             complement(2781..3296)
                     /gene="ogt"
                     /locus_tag="JNC24_004000"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HAY9160615.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLRDYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            complement(3307..>3735)
                     /locus_tag="JNC24_004001"
     CDS             complement(3307..>3735)
                     /locus_tag="JNC24_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate transporter"
                     /protein_id="HAY9160616.1"
                     /translation="KFIAVGLTDILESSGLSGIPAFVGLALLSSFLCMFIASGSAIWS
                     ILAPIFVPMFMLLGFHPAFAQILFRIADSSVLPLAPVSPFVPLFLGFLQRYKPDAKLG
                     TYYSLVLPYPLIFLVVWLLMLLAWYLVGLPIGPGIYPRLS"
BASE COUNT         1002 a          886 c          893 g          954 t
ORIGIN      
        1 taatgctacc aacagcactg ataattatgg tgtagattaa aaattgcacc gcatggaata
       61 aaaaaccttt tcttttacga tttccgcggc gaaaccaaaa gctcatcaga atcaatgctg
      121 cgcagctacc gaaaataatg accagattaa gcgcattatt tgtaaacagt tcagcgatca
      181 ttgttttatc aggctcctcc agataattgt cgtcatgccg gaaacccctg gcggggctat
      241 tttaccgcga caattcattc agatcatcaa tagtcaggga aggaagtagc aacattagct
      301 aactaatcaa tgcgttgatt gtaaatccag ctaagaggtg aggttttcag agcagacaac
      361 ggtgaaatgt catggtattg ttacgtttag gtaacaagaa atttgtctgc acaaggatta
      421 catcatgatt atggcgaaac tgaagtcagc gaaagggaaa aaatttctct ttggtttgtt
      481 ggcggttttc attattgcgg cgtcggttgt gactcgcgcg actatcggcg gcgttataga
      541 acagtacaat attccgctgt ctgagtggac gacatcaatg tatgtgattc agtcatcgat
      601 gatttttgtt tatagcctgg tctttactgt gttgctggca atcccgttgg gaatttattt
      661 ccttggcggc gaagagcagt aagtaaaaaa taggcccgat aactcgggcc ttgtcagtta
      721 ttgaagagtc gttaatcgtc ttcttcgtca tccagttcaa cgggtgtctg atactggtca
      781 ggtttaatga ccaacagatc gcagcgaaga tgatcaatca cctgttccgc cgtgttgccg
      841 aggaatgctg ctgaaatacc ggtgcgtcct accgtgccca gaaccacaat ccccgcctgt
      901 aagtgctccg ccaaatcagg aataacctct tccggcagac ctttttctac gtgcgtcatg
      961 ttttcattaa tgccgaattt ctgccgcaga gctttcattg ccagcaaatg ttgcccacga
     1021 atggcatcgt tataaacgct cggatcaaat tccggcagtt caatcgcgat attaattggc
     1081 gttactgggt aagcgccaac cagatgaact tcggtatggt tgacttgttc tgccagttcg
     1141 atcgtctctt tgaccagttt ttcattgagc gcattatgat acggctcttc actggcgaga
     1201 ttcaccgcca ccagcgcctt acctccttcc ggccacggct ggtctttcac catccacacc
     1261 gggcttgggc atttgcgtaa cagatgccag tccgttggcg taaaaatcac cgcttccaga
     1321 cggtcatgtt ggtgcgccat ttttagcacc aaatcgtgcc cgccgctgat cacttcctga
     1381 atgatggctt cgaagggacg gttatgccag accactttaa tttcaatggg aacgccagca
     1441 ttgagataat attttgcctg ctcgtggatc caggctgtac gctggctgat gacgccctga
     1501 cgcatagcgg tacgttcgtc cggggagagc agggtggtca tttcgtatga gaagtcatag
     1561 atcggcaaaa aggctttaat tttgccacca atccgttgat gtaaataaac agctcgccgc
     1621 aatgctggtt ggtcgtcctg gttaggatcg ataacaacga gcatgttctg atacatagcc
     1681 atacagggtc tccttacaac aactgtcaac gcagtttgta attaaaagat taacccatat
     1741 ctggtgaatg aaacagtgat gaaccttctg ccagatcaat aaatcagaaa aatttaatga
     1801 tatgacagaa ggatagtgag ttatgcggaa aaatcaggca acattacgcg tatgaccagc
     1861 aagctgggcc agcgcatcgt tattttcgat ggtgatgtat ttacctttga ctgccagcat
     1921 accgcttttc tggaagcgac ctagcagacg gctgatggtt tctaccgtca ggcccagata
     1981 gttaccgata tcgccacgag tcatcgtcag gcggaattca cgaggggaga agccgcgttg
     2041 ggcaaaacga cgggacaggt tgtagatgaa tgcagccaga cgttcctcgg catttttctt
     2101 cgacaacagc aagatcatgt cctgatcgcc tttgatttca ccgctcatca gacgcatcat
     2161 ctgctgacgc agattcggca ttttaccgga caaatcgtcc agcgtttcga acgggatttc
     2221 acataccatc gaggtttcca gcgcctgagc gaagctcggg tgatggccgc tgccgatggc
     2281 gtcaaatccc accaggtcgc cagctaaatg gaaaccagtg atttgctcgt cgccttgctc
     2341 agtgatggta taacttttaa tcgtaccgga gcggatggca taaagcgatt taagttcatc
     2401 accagcctta aacagcgtct ggcctttctg aataggcttc ttccgctcaa tgatattatc
     2461 aagctgatca agctcatgtt cgttgagtgt gaacgggatg caaagctggc tgatgctgca
     2521 atcctggcaa tggatagcac aaccgccaga ctgaatgcgc cgtataattc gcttttccgg
     2581 gatcataggt ctgctcaagc cgtaattgat atttgtcaat tttaacatct ttttagggag
     2641 caagtaagtc taagcaaacc ttaacagcag agaattccga tattagatgt aaatatatgt
     2701 ctatctattt gaaaaccctt aagttgttaa gggtaacttt acataaaagt gtgaacaagc
     2761 tggcacaaat tgtttaatgt ttacagcaaa agataacctt cgtggcgcaa taaccactct
     2821 tttcgctgaa ctccgcctgc atatccggtc atggtgccgt ttcggccaat aacccgatgg
     2881 caaggtacga cgatgctgat gggattcgat ccgtttgccg caccaacggc acgcgccgcg
     2941 ccagggcggc ccaattgttc agccagttgg ccgtaatgca ttacctgtcc gcaggggata
     3001 gtgcgtagag ttttccagac ttcgcgctga aatggcgtcc cccctgtggc agtgggaagc
     3061 gtatcaataa tgctaagatt accggcaaaa taatcacgaa gcttgtcgct taaaccgcct
     3121 gggttggtgg cagaaatgcg ctcatagcct tctttgcgat aatggatgtc cagcagctgc
     3181 accatgcgtt cgctgtactc ttcccattca accgcccgca ggcgaaattg ctcatcgcaa
     3241 atcacccaca gtggacccag tggcgtggca attttttctt caagtaatct cagcatccgt
     3301 tctctcttaa gacaaacgtg ggtaaatccc cggacctatc ggcagaccaa caagatacca
     3361 cgccagcaac atcagcagcc ataccaccaa aaagataagc ggatagggca agactaacga
     3421 atagtaagta cccagtttcg cgtctggttt gtagcgttgc aggaatccaa gaaacagtgg
     3481 aacaaaagga gataccggcg ctaaaggcaa tacggatgag tcggcaatac gaaagaggat
     3541 ttgcgcaaat gccgggtgaa agccaagtag cataaacatt ggtacgaaaa tgggggccag
     3601 aatcgaccag attgcggaac cgctggcaat aaacatgcat aagaaagagg aaagcaacgc
     3661 cagaccgaca aacgccggga tgccgctaag ccctgaactt tccagtatat cggtcagccc
     3721 cacggcgatg aattt
//
LOCUS       DADMKF010000255         3699 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.260, whole genome shotgun sequence.
ACCESSION   DADMKF010000255 DADMKF010000000
VERSION     DADMKF010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3699
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.260"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            2..394
                     /locus_tag="JNC24_004002"
     CDS             2..394
                     /locus_tag="JNC24_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAY9160617.1"
                     /translation="MLFPNFSLGEEYEHAPPATNRQISPYLPSGRFRTGLPVEGLAIE
                     RGDLFYACPRASVFYGTALDADLRTRGVSTLVMAGISTTGVVLSSVAWASDADYDVRL
                     VQDCCYDPDRDAHEALLRSGFGGRVQVV"
     gene            532..1416
                     /locus_tag="JNC24_004003"
     CDS             532..1416
                     /locus_tag="JNC24_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HAY9160618.1"
                     /translation="MSLRTPDLLFTAIAPAIWGSTYIVTTQYLPNFSPMTVAMLRALP
                     AGLLLVMIVRQIPTGIWWMRIFILGALNISLFWSLLFISVYRLPGGVAATVGAVQPLM
                     VVFISAALLGSPIRLMAVLGAICGTAGVALLVLTPNAALDPVGVAAGLAGAVSMAFGT
                     VLTRKWQPPVPLLTFTAWQLAAGGLLLVPVALVFDPPIPMPTGTNVLGLAWLGLIGAG
                     LTYFLWFRGISRLEPTVVSLLGFLSPGTAVLLGWLFLDQTLSALQIIGVLLVIGSIWL
                     GQRSNRTPRARIACRKSP"
     gene            complement(1448..2647)
                     /gene="tet(A)"
                     /locus_tag="JNC24_004004"
     CDS             complement(1448..2647)
                     /gene="tet(A)"
                     /locus_tag="JNC24_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(A)"
                     /protein_id="HAY9160619.1"
                     /translation="MKPNRPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAH
                     YGILLALYALMQFACAPVLGALSDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYIG
                     RIVAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPH
                     APFFAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAV
                     FFIMQLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGE
                     RRALMLGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQG
                     QLQGSLAALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSG
                     AGQRADR"
     gene            2753..3403
                     /gene="tetR(A)"
                     /locus_tag="JNC24_004005"
     CDS             2753..3403
                     /gene="tetR(A)"
                     /locus_tag="JNC24_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(A)"
                     /protein_id="HAY9160620.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWH
                     FRNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAG
                     TRPGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGG
                     TVEQAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     gene            complement(3435..3677)
                     /locus_tag="JNC24_004006"
     CDS             complement(3435..3677)
                     /locus_tag="JNC24_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase"
                     /protein_id="HAY9160621.1"
                     /translation="MKTAALDLARERQAHEAGARTRATAHERTPQQERQKAAREAERG
                     REAWTLGQGMKKPVAGCYGRLTRWKGGGDVVYMALL"
BASE COUNT          686 a         1144 c         1162 g          707 t
ORIGIN      
        1 cgtgctgttc cccaactttt cgctcggcga agagtatgaa catgcgccgc cggcgacgaa
       61 tcgccaaatc tcaccgtatc tcccgagcgg acgatttcgc accggtctgc ccgtcgaagg
      121 gcttgcgatc gaacggggcg accttttcta tgcatgtccg cgagccagcg tcttttatgg
      181 cacggcgctc gacgccgacc ttcggacgcg cggcgtaagc acgcttgtca tggccgggat
      241 aagcaccacc ggcgttgttc tttcaagcgt cgcctgggct agtgatgcgg actacgacgt
      301 gcgtttggtc caggactgct gctacgaccc ggatcgggat gcccacgaag ctctgttgcg
      361 ttccgggttc ggcggacgtg tacaggtcgt gtaattttcg ggcctcgcat gatcgcggcc
      421 atagccgcag gtggattggt gcgaggcagg gccagtccaa gtcagggtgc ggtcatcgcg
      481 gcgcctgcaa ctctcaaaaa cccatcgccg tagcggaggt cttgtaatcg catgtcatta
      541 cgcacgcccg atttattgtt cacagcgata gcacctgcca tttggggcag cacctacatt
      601 gtcaccaccc aatacctgcc gaacttctca ccgatgacgg tcgcgatgct gcgggcgttg
      661 ccggcgggtt tattgctcgt gatgatcgtc cgacagattc caacgggaat ctggtggatg
      721 cgcatcttca tcctcggcgc acttaatatt tcgctattct ggagcttgtt gtttatttcg
      781 gtctaccgcc tgccgggcgg ggtcgcggcg acggtaggcg ctgtgcagcc gctgatggtc
      841 gtgttcatct ctgccgctct gctaggtagc ccgatacgat tgatggcggt cctgggggct
      901 atttgcggaa ctgcgggcgt ggcgctgttg gtgttgacac caaacgcagc gctagatcct
      961 gtcggcgtcg cagcgggcct ggcgggggcg gtttccatgg cgttcggaac cgtgctgacc
     1021 cgcaagtggc aacctcccgt gcctctgctc acctttaccg cctggcaact ggcggccgga
     1081 ggacttctgc tcgttccagt agctttagtg tttgatccgc caatcccgat gcctacagga
     1141 accaatgttc tcggcctggc gtggctcggc ctgatcggag cgggtttaac ctacttcctt
     1201 tggttccggg ggatctcgcg actcgaacct acagttgttt ccttactggg ctttctcagc
     1261 ccggggaccg ccgtgttgct aggatggttg ttcttggatc agacgctgag tgcgcttcaa
     1321 atcatcggcg tcctgctcgt gatcgggagt atctggctgg gccaacgttc caaccgcact
     1381 cctagggcgc gtatagcttg ccggaagtcg ccttgacccg catggcatag gcctatcgtt
     1441 tccacgatca gcgatcggct cgttgccctg cgccgctcca aagcccgcga cgcagcgccg
     1501 gcaggcagag caagtagagg gcagcgcctg caatccatgc ccacccgttc cacgttgtta
     1561 tagaagccgc atagatcgcc gtgaagagga ggggtccgac gatcgaggtc aggctggtga
     1621 gcgccgccag tgagccttgc agctgcccct gacgttcctc atccacctgc ctggacaaca
     1681 ttgcttgcag cgccggcatt ccgatgccac ccgaagcaag caggaccatg atcgggaacg
     1741 ccatccatcc ccgtgtcgcg aaggcaagca ggatgtagcc tgtgccgtcg gcaatcattc
     1801 cgagcatgag tgcccgcctt tcgccgagcc gggcggctac agggccggtg atcattgcct
     1861 gggcgagtga atgcagaatg ccaaatgcgg caagcgaaat gccgatcgtg gtcgcgtccc
     1921 agtgaaagcg atcctcgccg aaaatgaccc aaagcgcggc cggcacctgt ccgacaagtt
     1981 gcatgatgaa gaagaccgcc atcagggcgg cgacgacggt catgccccgg gcccaccgga
     2041 acgaagcgag cgggttgaga gcctcccggc gtaacggccg gcgttcgcct ttgtgcgact
     2101 ccggcaaaag gaaacagccc gtcaggaaat tgaggccgtt caaggctgcc gcggcgaaga
     2161 acggagcgtg gggggagaaa ccgcccatca gcccaccgag cacaggtccc gcgaccatcc
     2221 cgaacccgaa acaggcgctc atgaagccga agtgccgcgc gcgctcatcg ccatcagtga
     2281 tatcggcaat ataagcgccg gctaccgccc cagtcgcccc ggtgatgccg gccacgatcc
     2341 gcccgatata gagaacccaa aggaaaggcg ccgtcgccat gatggcgtag tcgacagcag
     2401 cgccggccag cgagacgagc aagaccggcc gccgcccgaa acgatccgac agcgcgccca
     2461 gcacaggtgc gcaggcaaat tgcatcaacg catacagcgc cagcagaatg ccatagtggg
     2521 cggtgacgtc gttcgagtga accagatcgc gcaggaggcc cggcagcacc ggcataatca
     2581 ggccgatgcc gacagcgtcg agcgcgacag tgctcagaat tacgatcagg ggtctgttgg
     2641 gtttcacgtc tggcctccgg accagcctcc gctggtccga ttgaacgcgc ggattcttta
     2701 tcactgataa gttggtggac atattatgtt tatcagtgat aaagtgtcaa gcatgacaaa
     2761 gttgcagccg aatacagtga tccgtgccgc cctggacctg ttgaacgagg tcggcgtaga
     2821 cggtctgacg acacgcaaac tggcggaacg gttgggggtt cagcagccgg cgctttactg
     2881 gcacttcagg aacaagcggg cgctgctcga cgcactggcc gaagccatgc tggcggagaa
     2941 tcatacgcat tcggtgccga gagccgacga cgactggcgc tcatttctga tcgggaatgc
     3001 ccgcagcttc aggcaggcgc tgctcgccta ccgcgatggc gcgcgcatcc atgccggcac
     3061 gcgaccgggc gcaccgcaga tggaaacggc cgacgcgcag cttcgcttcc tctgcgaggc
     3121 gggtttttcg gccggggacg ccgtcaatgc gctgatgaca atcagctact tcactgttgg
     3181 ggccgtgctt gaggagcagg ccggcgacag cgatgccggc gagcgcggcg gcaccgttga
     3241 acaggctccg ctctcgccgc tgttgcgggc cgcgatagac gccttcgacg aagccggtcc
     3301 ggacgcagcg ttcgagcagg gactcgcggt gattgtcgat ggattggcga aaaggaggct
     3361 cgttgtcagg aacgttgaag gaccgagaaa gggtgacgat tgatcaggac cgctgccgga
     3421 gcgcaaccca ctcactacag cagagccatg tagacaacat cccctccccc tttccaccgc
     3481 gtcagacgcc cgtagcagcc cgctacgggc tttttcatgc cctgccctag cgtccaagcc
     3541 tcacggccgc gctcggcctc tctggcggcc ttctggcgct cctgctgcgg cgtccgctcg
     3601 tgggccgtgg cgcgggtccg cgcgccggcc tcgtgcgcct ggcgctcgcg ggcgaggtcc
     3661 agggcggccg tcttcacgtt ctgccttgcg cagatgaga
//
LOCUS       DADMKF010000256         3674 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.261, whole genome shotgun sequence.
ACCESSION   DADMKF010000256 DADMKF010000000
VERSION     DADMKF010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3674
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.261"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            <1..95
                     /locus_tag="JNC24_004007"
     CDS             <1..95
                     /locus_tag="JNC24_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HAY9160622.1"
                     /translation="RLGRKSLSFSKSVELHDKVIGHYLNIKHYQ"
     gene            244..1491
                     /locus_tag="JNC24_004008"
     CDS             244..1491
                     /locus_tag="JNC24_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY9160623.1"
                     /translation="MALNIPFRNAYYRFASSYSFLFFISWSLWWSLYAIWLKGHLGLT
                     GTELGTLYSVNQFTSILFMMFYGIVQDKLGLKKPLIWCMSFILVLTGPFMIYVYEPLL
                     QSNFSVGLILGALFFGLGYLAGCGLLDSFTEKMARNFHFEYGTARAWGSFGYAIGAFF
                     AGIFFSISPHINFWLVSLFGAVFMMINMCFKDKDHQCVAADAGGVKKEDFIAVFKDRN
                     FWVFVIFIVGTWSFYNIFDQQLFPVFYAGLFESHDVGTRLYGYLNSFQVVLEALCMAI
                     IPFFVNRVGPKNALLIGVVIMALRILSCALFVNPWIISLVKLLHAIEVPLCVISVFKY
                     SVANFDKRLSSTIFLIGFQIASSLGIVLLSTPTGILFDHAGYQTVFFAISGIVCLMLL
                     FGIFFLSKKREQIVMETPVPSAI"
     gene            complement(2020..2220)
                     /gene="torI"
                     /locus_tag="JNC24_004009"
     CDS             complement(2020..2220)
                     /gene="torI"
                     /locus_tag="JNC24_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator inhibitor TorI"
                     /protein_id="HAY9160624.1"
                     /translation="MQHELQPDSLVDLKFIMADTGFGKTFIYDRIKSGDLPKAKVIHG
                     RARWLYRDHCEFKNKLLSRANG"
     gene            complement(2343..2687)
                     /locus_tag="JNC24_004010"
     CDS             complement(2343..2687)
                     /locus_tag="JNC24_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9160625.1"
                     /translation="MSDLAMKVLKWQSTGDVGISSATLASIACGLKKNIYGHHFGALH
                     DAADFRRCVALVEQIPEIRDSFDKVAKRVPAFKGILNEWDSLVALLKSEMKTYGNKAP
                     ETYRRISELRKD"
     gene            2929..3531
                     /locus_tag="JNC24_004011"
     CDS             2929..3531
                     /locus_tag="JNC24_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9160626.1"
                     /translation="MSYFLCKKWMINLSGAGKILWALNMKKDSYPYLICMTVSGLIFI
                     FLFFWWRADIYRVTFLNQSISHYYILFSMGIAFLLSLFWVKKGIVKQSGWKSLSAYLK
                     VYAGICIFSGFFLIIPLTTLTYFLPGETSSYVAPYRYTSGSSKSCSGAEVDDPDLHEN
                     IRICYPYGNYEYDNIIYVEKKINTLGAVVTYAQTARDDTE"
BASE COUNT          919 a          740 c          845 g         1170 t
ORIGIN      
        1 cacggctggg acggaagtcg ctgtcgttct caaaatcggt ggagctgcat gacaaagtca
       61 tcgggcatta tctgaacata aaacactatc aataagttgg agtcattacc cgcttgcagt
      121 aacggcgaaa ggggcaatga cagcgctgcc atgtcgacaa gaactggaat agtgagaagt
      181 aaacggcgaa gtcgctctta tctctaaata ggacgtgaat tttttaacga caggtaggta
      241 attatggcac tgaatattcc attcagaaat gcgtactatc gttttgcatc cagttactca
      301 tttctctttt ttatttcctg gtcgctgtgg tggtcgttat acgctatttg gctgaaagga
      361 catctagggt tgacagggac ggaattaggt acactttatt cggtcaacca gtttaccagc
      421 attctattta tgatgttcta cggcatcgtt caggataaac tcggtctgaa gaaaccgctc
      481 atctggtgta tgagtttcat cctggtcttg accggaccgt ttatgattta cgtttatgaa
      541 ccgttactgc aaagcaattt ttctgtaggt ctaattctgg gagcgctctt ttttggcctg
      601 gggtatctgg cgggatgcgg tttgcttgac agcttcaccg aaaaaatggc gcgaaatttt
      661 catttcgaat atggaacagc gcgcgcctgg ggatcttttg gctatgctat tggcgcgttc
      721 tttgccggca tattttttag tatcagtccc catatcaact tctggttggt ctcgctattt
      781 ggcgctgtat ttatgatgat caacatgtgt tttaaagata aggatcacca gtgcgtagcg
      841 gcggatgcgg gaggggtaaa aaaagaggat tttatcgcag ttttcaagga tcgaaacttc
      901 tgggttttcg tcatatttat tgtggggacg tggtctttct ataacatttt tgatcaacaa
      961 ctttttcctg tcttttatgc aggtttattc gaatcacacg atgtaggaac gcgcctgtat
     1021 ggttatctca actcattcca ggtggtactc gaagcgctat gcatggcgat tattcctttc
     1081 tttgtgaatc gggtagggcc aaaaaatgca ttacttatcg gtgttgtgat tatggcgttg
     1141 cgtatccttt cctgcgcgct gttcgttaac ccctggatta tttcattagt gaagctgtta
     1201 catgccattg aggttccact ttgtgtcata tccgtcttca aatacagcgt ggcaaatttt
     1261 gataagcgcc tgtcgtcgac gatctttctg attggttttc aaattgccag ttcgcttggg
     1321 attgtgctgc tttcaacgcc gactgggata ctctttgacc acgcaggcta ccagacagtt
     1381 ttcttcgcaa tttcgggtat tgtctgccta atgttgctat ttggcatttt cttcctgagt
     1441 aaaaaacgcg agcaaatagt tatggaaacg cctgtacctt cagcaatata gacgtaaact
     1501 tttccggttg ttgtcgatat ctccctatcc ctcaaccgga aaataataat actaaagtgc
     1561 ttagcccggc taataatcac ctaatccaaa cgcctcattc atgttctggt acagtcgctc
     1621 aaatgtactt cagatgcgcg gttcgctgat ttccaggaca ttgtcgtcat tcagcgacct
     1681 gtcccgtgta tcacggtcct gcgaattcat caaggaatgc attgcggagt gaagtatcga
     1741 gtcacgccat atttcgctat caggattctg tgtgatggtt acatcgcccg gcccagggct
     1801 gtttagtcat cagcgctttc tgacagtgct gagatttcaa cctgttgcag taaaaatgag
     1861 tagatataag gcaagtgtgc tgccaaaccc atcttttacg gggtgaaggt agatttcgtt
     1921 tgaagggtat ctggtgtccc ctgcagacat ctacttgagg cggcagggga ttgattggaa
     1981 tgctgttttt tagatttgaa aaatatttta cccgctattt tacccattgg cgcggcttaa
     2041 gagcttattt ttgaattcac aatggtcacg atataaccat cttgctcgtc cgtggataac
     2101 tttggcttta ggcaggtcgc cggacttaat ccggtcatag atgaaggtct taccgaagcc
     2161 agtatcagcc atgatgaatt tcaaatcaac cagtgaatca ggttgtagtt cgtgttgcat
     2221 gagtgctatc tccgaatatg gaatcgaacc tgcaaatcag gcaataaaaa gccgcattga
     2281 tgcagcaatg gtaggtctgg atatcttgag aaatgaacag gcctcatcga gtgtgaggcg
     2341 ggttagtcct tgcgtagctc gctgattctt ctgtaagtct ctggtgcttt gttcccgtac
     2401 gtcttcattt cagacttcaa cagagcaacg agtgaatccc attcgttgag gattcctttg
     2461 aatgccggaa cgcgctttgc aaccttgtcg aatgaatctc tgatttctgg aatctgctca
     2521 acaagtgcaa cgcatcgccg aaagtctgct gcgtcatgga gagcgccgaa gtgatgacca
     2581 tagatattct ttttcaggcc acatgcgatt gaggcaagag ttgcgctact gatgccgaca
     2641 tcgccagttg attgccattt caaaaccttc atagccaaat ctgacatttc ttgtctccat
     2701 aaaacaaaac tcgccgtagc gagctcagat aaaagaaatc cccgtcagtg cgaggatgct
     2761 gttcattgct gctatacact tttttgctct caacgtaagc ggtagcccat tctgttgggt
     2821 tggtcagttg cttttaggaa atgctcttta ccccttaaac gtcggctgaa agagctaaaa
     2881 tccatgcaaa aaatttacgc aattttgtgt attattgtgc agtaagtaat gagctatttt
     2941 ctgtgcaaaa aatggatgat aaatttgtcc ggggcaggaa aaattttatg ggcgctaaac
     3001 atgaaaaaag attcgtatcc ttatttgatt tgcatgacag tttcagggct gatctttatt
     3061 ttccttttct tctggtggcg ggcagatatc tacagggtca cgtttcttaa tcagagtata
     3121 tcccactatt acattctgtt tagcatggga atagcttttc tgttatctct gttttgggtt
     3181 aagaagggga tagtaaaaca aagcggctgg aagagtctgt cagcatacct taaggtttat
     3241 gcagggatat gcatattttc tggatttttt ctgattatac cccttacaac actaacttat
     3301 tttttgcctg gagagacatc gtcttatgtt gcaccgtatc ggtatacttc cggtagttca
     3361 aaaagttgtt ctggagctga ggtggatgac cccgatctac atgagaatat tcgcatttgc
     3421 tatccgtatg gcaattatga gtacgataat attatctatg ttgaaaagaa aattaataca
     3481 ttaggtgcgg tagtgacata tgcacagacc gcgcgtgatg atactgaatg agatagtata
     3541 tagcgggcaa gttttagtta atttatcgag gtaatataat ttacctcgac tcgtttattc
     3601 tggtatgaat atttcgcttt acgatcgatt tttatctgat gatatcatgc ggttttcata
     3661 tactgactta ctgt
//
LOCUS       DADMKF010000257         3627 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.262, whole genome shotgun sequence.
ACCESSION   DADMKF010000257 DADMKF010000000
VERSION     DADMKF010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3627
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.262"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(89..652)
                     /locus_tag="JNC24_004012"
     CDS             complement(89..652)
                     /locus_tag="JNC24_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HAY9160627.1"
                     /translation="MNRTSIEKLPSLTHNHLPVMTTELLADLYGTERQRLTNNFNRNK
                     ERFIEGKHFFLIEGDALRELKNENSLRVSVKIARNVRSLILWTERGAARHAKMLETDQ
                     AWEVFEKLEDCYFNQKQPPAAQNTSIENDGCALLSHFDKHGQVEFTEKVPADAMVCTL
                     ERFKFYLEQHGWIVARKEQLVERLMRF"
     gene            complement(756..974)
                     /locus_tag="JNC24_004013"
     CDS             complement(756..974)
                     /locus_tag="JNC24_004013"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9160628.1"
                     /translation="MGELKSSPVGFPVVPTLCVSPPDDWNLTVLTVSGLQFTFPRGAQ
                     NTSKAVSTPDSHVVFLCPEFLVYDRVCG"
     gene            complement(1133..1519)
                     /locus_tag="JNC24_004014"
     CDS             complement(1133..1519)
                     /locus_tag="JNC24_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HAY9160629.1"
                     /translation="MARDIQQVMERWGAWAANTHEDVSWASIAAGFKGLIPSKVKSRP
                     QCSDDDAMIICGCMARLNKKNQDLHDLLVDYYVGGMTFMSLARKHGCSDTCIGKRLQK
                     AEGVIDGMLMMLDIRLEMDRYVERIM"
     gene            complement(1509..1880)
                     /locus_tag="JNC24_004015"
     CDS             complement(1509..1880)
                     /locus_tag="JNC24_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HAY9160630.1"
                     /translation="MRHEFILPYPPTVNTYWRRRGSTYFVSKVGERYRRDVTLIVRQQ
                     RLKLNLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDEINIVRGQ
                     LVPGGRLGVKIYEITGDNDGA"
     gene            complement(1880..2137)
                     /locus_tag="JNC24_004016"
     CDS             complement(1880..2137)
                     /locus_tag="JNC24_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9160631.1"
                     /translation="MNNQTMTFTSEQLRKHAQEMLRQAEQLEKTGVTKDAIRRDMVPA
                     LRELMQAKHRAQKAVDELVDCVAELETKVGKFEKMVQEVLR"
     gene            complement(2134..3525)
                     /locus_tag="JNC24_004017"
     CDS             complement(2134..3525)
                     /locus_tag="JNC24_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HAY9160632.1"
                     /translation="MTTPVWRNDDLEGAVIGAFFLRGADHEVMDILITLPADIFSVRA
                     YRDIYTGICRQARVSGVIDPVLLCNEMPELAPVITDTGRKTWVKSSLEHYVAALRRNA
                     ALRDAEKTLNEALQKLRDAHTCEAAEDALKDAQNMMVTLSTGKGVIQPVHIDDVLPEV
                     VERVECRNQGLEKSRTLMTGIDELDAKTGGMEPGDLVFIAARPSMGKTELALDIIDKV
                     TEQGHGVLLFTMEMANIQIGERMVSAAGGMPVSRLKSVAHFEDEDWARFSQGVGRMTG
                     RNIWMVDQANLTIDEICATTKHHLIKHPETALVVVDYLGLIKTRTTGRHDLAVGEISK
                     GLKGLAKSGGFPLIALSQLSRGVESRPNKRPMNSDLKNSGEIEADADIILMLYRDEVY
                     NPDTQATGIAEINITKQRNGSLGTIYRRFYNGHFLPVDQESAQVLSTPMRQPQPRRYS
                     NTRTDSSKMERFF"
BASE COUNT          850 a         1013 c          811 g          953 t
ORIGIN      
        1 catcggcggg tttaagctgt gtggcgtagt aaccactctt aacaggatat tcaacttttt
       61 acgatcgtaa agttttcgga aaaaattttt aaaaccgcat caaccgctcc accagttgct
      121 ctttacgggc aacgatccac ccgtgttgct ccagataaaa tttaaaccgt tccagagtac
      181 ataccatcgc atcggcgggt accttttccg tgaactcaac ctgaccgtgt ttatcgaagt
      241 ggctcagtaa tgcgcatcca tcattttcga tagatgtgtt ttgtgccgct ggtggctgtt
      301 tctggttgaa ataacagtct tccagttttt cgaacacttc ccacgcctga tcggtttcga
      361 gcattttggc gtgacgggct gcgccacgtt ctgtccagag gatgagggag cgaacgttac
      421 gggcaatttt cacagaaact cttaaagagt tttcgttctt caactctcgt aacgcatcac
      481 cttctatcag gaaaaaatgt ttaccttcta taaaccgttc tttgtttcga ttgaaattgt
      541 ttgtcaaacg ctggcgctct gttccgtaca aatcagccag cagctctgtt gtcatgacag
      601 gaagatggtt atgcgtaagt gacgggagtt tttcaataga cgtgcgattc atagacatgt
      661 cctttcgaat attttgattt accccttttg agagggtgac cgggcgctca aaaccgtcga
      721 aagtcggcgg gcgtatttcc attgctggta ttgtattagc cgcacacccg gtcataaacc
      781 aagaattctg gacacaaaaa aaccacatgg ctatcgggtg tggataccgc tttcgaggtg
      841 ttttgagcac cgcggggaaa tgtaaactgc aaacctgaaa ctgtcaacac cgtcaggttc
      901 caatcatcgg gtggtgagac gcacagggtt ggaactaccg ggaaaccgac cggcgagctt
      961 ttcagctccc ccatgcgccc caccataatt cagatgtgcg cgtgcatacg acaataaaaa
     1021 acacgctcgc ggcgtgtgtc tgtcgcggtc tctatccggg gttccaatcc cgacgccaga
     1081 ttttgctggc gcgtgaggaa tatagccccg gacaatgtgt ctggtcaagc acctacataa
     1141 ttcgttctac gtatctgtcc atctccagcc ggatatcaag catcatcaac atgccatcaa
     1201 taaccccttc cgctttctgc aggcgcttgc caatacaggt atccgaacac ccatgtttcc
     1261 gtgccagact cataaaagtc attccaccta cgtaataatc caccagcaaa tcgtgcaaat
     1321 cctgattttt cttgttcaac cgggccatac agccacaaat tatcattgca tcatcatcag
     1381 aacactgagg gcgtgatttc actttcgacg gaattaaccc tttaaaacca gcagcgatcg
     1441 acgcccagga tacatcttca tgagtgtttg cagcccaagc cccccaccgt tccataacct
     1501 gctgaatatc acgcgccatc gttatcacct gtgatttcat aaatcttcac gcccagtcgc
     1561 ccaccaggaa cgagctgacc gcgcacaata ttgatttcat caaactgctc gtcgtctatg
     1621 agtagtccgg catgcgtcag cgcatccagt ggtgctttca ggatattgtc caggtcacga
     1681 cggcgcttat ccggtggctc tgcaataatc tttatcgcca gccttccgga caggtttaat
     1741 ttcagccgct gctggcgaac aattagcgtc acatcacggc gataacgctc accaactttt
     1801 gatacaaaat atgtgctgcc acgacgtcgc cagtaagtat tcaccgtcgg cggataaggt
     1861 aaaataaact catggcgcat cagcgcagca cctcctgcac cattttttca aactttccaa
     1921 ctttggtttc cagctctgcc acacaatcaa cgagctcatc caccgctttt tgtgcgcgat
     1981 gtttcgcctg catcagttcc ctgagcgctg gcaccatatc ccgacgaatg gcatcttttg
     2041 ttacacctgt tttttccagt tgttccgcct gtcgcaacat ttcctgcgcg tgtttacgca
     2101 attgttcaga ggtaaaagtc attgtctggt tgttcaaaag aaacgctcca tcttactgct
     2161 gtcggtacgt gtattgctgt atctgcgcgg ctggggctgc cgcattgggg tggaaagaac
     2221 ctgtgcgctt tcctggtcca caggcagaaa atgtccgtta taaaaacgcc ggtaaatcgt
     2281 ccccagagaa ccgttacgtt gtttcgtgat attaatttct gctatgcctg tagcctgtgt
     2341 atccgggttg tacacttcat ccctgtaaag catcagaatg atgtctgcat ccgcctctat
     2401 ttctccggaa tttttcaggt ctgagttcat gggacgttta ttgggtctgg actccacacc
     2461 gcgggagagc tggctcagcg caatcaacgg aaaaccaccg gattttgcca ggcctttaag
     2521 cccctttgag atttcaccca cggcaaggtc atgacgcccc gtggttcggg tttttatcag
     2581 cccgagataa tcaaccacca ccagcgccgt ttccggatgt ttaatcagat ggtgtttcgt
     2641 tgttgcgcat atctcatcaa tggtcaggtt cgcctggtcc accatccaga tattgcgccc
     2701 ggtcatccgc cccacccctt gtgagaaacg cgcccagtct tcatcttcaa agtgagccac
     2761 agatttcagg cgtgatactg gcatccctcc agccgcagac accatacgtt caccaatctg
     2821 gatgttcgcc atctccatgg tgaacagaag cacaccatgc ccctgctcag tcaccttgtc
     2881 gatgatgtcc agcgcaagtt cggttttccc catcgaagga cgggcggcaa tgaataccag
     2941 gtctccgggc tccataccgc ctgtttttgc gtccagttca tcaataccgg tcatcaacgt
     3001 cctggatttc tccagcccct gattccggca ttcaacacgc tcaaccactt ccggaagcac
     3061 atcatcaata tgtaccggct gaatgacgcc ctttcctgtc gacagtgtga ccatcatgtt
     3121 ctgcgcatcc ttcagggcat cttcagctgc ttcacaggta tgcgcatcac gtaatttctg
     3181 cagcgcctca ttcagtgttt tttctgcatc gcgcagtgcg gcattgcgcc gcaacgctgc
     3241 aacatagtgc tccagtgaag acttcaccca ggttttacgc ccggtatcag taatcaccgg
     3301 ggcaagttcc ggcatctcat tacacaacag cacggggtca atcactcctg aaacacgggc
     3361 ctgtctgcag atgcctgtgt agatatcccg atacgctcgt acagaaaaaa tgtccgccgg
     3421 tagtgtgatc agaatatcca tcacttcatg atctgcccca cgcagaaaga atgcgccaat
     3481 gacagcgcct tccaggtcat cattacgcca gactggtgtt gtcatgcagc cacacctctg
     3541 atacaagaac ggtagctggg ccagttgaac gacaaccagt tgcgcccccc gtctgtgatc
     3601 ctgtcggcaa tgcgggggct gatgaac
//
LOCUS       DADMKF010000258         3577 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.263, whole genome shotgun sequence.
ACCESSION   DADMKF010000258 DADMKF010000000
VERSION     DADMKF010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3577
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.263"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            <1..196
                     /locus_tag="JNC24_004018"
     CDS             <1..196
                     /locus_tag="JNC24_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9160633.1"
                     /translation="SPKKIGFYTIGDKSIYLYDLRRMDEIMEALDNRSSMDWCVAVHD
                     MNAGFDEKILFPSSVESTAG"
     gene            371..715
                     /locus_tag="JNC24_004019"
     CDS             371..715
                     /locus_tag="JNC24_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9160634.1"
                     /translation="MTTFTREQLIAHAEETIEAQRLCIPGTIDHDIIRTYKMDIAVLE
                     IALVSLAAEPAGKLHEYKPVGYQRLVDELTMLVKQLTWQLRKAKPDCKLPDKAMSYLE
                     RNGLISVEDILR"
     gene            950..1162
                     /locus_tag="JNC24_004020"
     CDS             950..1162
                     /locus_tag="JNC24_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HAY9160635.1"
                     /translation="MNGKSRLASYVPKGKEKQAMKQQKAMLIALIVICLTVIVTALVT
                     RKDLCEVRIRTGQTEVAVFTAYEPEE"
     gene            1466..1738
                     /locus_tag="JNC24_004021"
     CDS             1466..1738
                     /locus_tag="JNC24_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9160636.1"
                     /translation="MPSRLHNSDNSAVFASRHGRRSHAFKSDWYQHPPCTEEQAEWLI
                     QCYRRRGCEVKKALSLDYRHWIISVRLPYSERPTRPSRTFQQRIWR"
     gene            1812..2798
                     /locus_tag="JNC24_004022"
     CDS             1812..2798
                     /locus_tag="JNC24_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HAY9160637.1"
                     /translation="MSAFHNGRILVEPEPKSMRNLPSGVVPAARQPLVEDKTLLPFFS
                     NARVIRAAGGAGALSDWLLRHIKSCQWPHGDYHHSETVIHRYGTGAMVLCWHCDNQLR
                     DQTSESLEQLAHQNLSAWMIDVIGHAISGTQERELSLAELSWWAVRNQVADALPEAVL
                     RRSLGLRAEKIRSVYSESDIIPGEQTATSILKQRTKNIALLPHVHQQQNPPQEKTVVS
                     IAVDPESPAQYLQRQKSQREEMPVYTRWVKTQKCMTCGNQADDPHHIIGHGLGGMGTK
                     ADDLFVIPLCRKCRNELHAGVKDFEEKHGSQLLLLIRFLMHARNSGVLKWKA"
     gene            2799..3179
                     /locus_tag="JNC24_004023"
     CDS             2799..3179
                     /locus_tag="JNC24_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HAY9160638.1"
                     /translation="MTERIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRAVALIVR
                     QQRLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLMDDEQFDEINIVR
                     AQPVSGGRLGVKIYPIMLEGQVKK"
     gene            3176..>3577
                     /locus_tag="JNC24_004024"
     CDS             3176..>3577
                     /locus_tag="JNC24_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HAY9160639.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGVSAFLGKMGISMNDRERATELLTEYALSRCDRVAALRKLPAEIKPAVMRIMASY
                     AFEDYARSAASKKQCPCCHGKKFIESEVFTNK"
BASE COUNT          969 a          808 c          979 g          821 t
ORIGIN      
        1 atcaccgaaa aaaattggct tttacaccat tggcgataaa agcatttacc tgtatgacct
       61 acgccgcatg gatgaaatca tggaggctct tgataatcgt tcgtcgatgg attggtgtgt
      121 tgctgttcat gatatgaatg cagggtttga tgaaaagatt ttgttcccct catcagttga
      181 aagcactgcg ggttaaggag taacacatga ccactattac caaagaacgt attgaattgt
      241 tcattaaaaa cccgcttgaa aacgggctta cccgtggtga acaaatggaa ctggcacgga
      301 ttgcgctggc atcgctggaa gcagagccgg ttgtgttctg gtttgaaaaa tatcaagaag
      361 gggctacggc atgacgactt ttaccagaga gcagttaata gctcacgcag aggagactat
      421 tgaagcacag agactgtgca taccgggcac aatcgaccat gacatcatcc gcacatataa
      481 gatggatatt gctgttctgg aaatcgcact ggtatcgctg gcagcagagc cagccggtaa
      541 attgcatgaa tacaaaccag tgggatatca gcgtctggtc gatgagttaa ccatgctggt
      601 aaagcagtta acctggcaac tgaggaaagc gaagccagac tgcaaattac cggataaggc
      661 gatgagttat ctggagcgga acggactgat aagcgtggag gatattttac gatgacctag
      721 cctgaagcat taacaacggt aggaattgcg atggcggtgg cgctggtggt gtattcgatt
      781 tgccgctggg gataaaaacg gtttgcggga aaaggagagt taagtagaat tgcagcgggt
      841 gcttgaggct atctgtctca ggcatgaaca ccaaaaggca gatagagaaa agccccagtt
      901 aacattacgc gtccggcaag acgcttaaca ttaatctgag gctcaatcta tgaacggcaa
      961 atctaggtta gcctcttacg tgccgaaagg caaggagaag caggctatga agcagcaaaa
     1021 ggcgatgtta atcgccctga tcgtcatctg tttaaccgtc atagtgacgg cactggtaac
     1081 gaggaaagac ctctgcgagg tacgaattcg aaccggccag acggaggtcg ctgtcttcac
     1141 agcttacgaa cctgaggagt aagagaccag gcgggggaga aatccctcgc cacctctgat
     1201 gggtcaggca tcctcaatgc acccacactt aacccgcttc ggcgggtttt tgtttttatt
     1261 ttcaacgcgt ttgaagtttt agatggtgcc ggaatagaat caaaaatact taagtagcgc
     1321 gcagggagaa gagggatgga ccccgaacag gggagtgcta tttatctgga aggattctgt
     1381 tgatgaaaat cgaagaatta cgtgaaattt ttagtgaaga tggcctctat actgtgcgcg
     1441 ttgagaatgg cgctattgtc agccactgcc ttccagacta cacaattctg ataattcagc
     1501 cgtctttgcc agcaggcacg ggcggcgctc tcacgcattt aaatctgatt ggtaccagca
     1561 tcccccatgc actgaagaac aggccgaatg gctcattcag tgttaccgca ggcgcggatg
     1621 cgaggttaaa aaagccctta gcctcgacta ccgtcactgg ataatctccg tcaggctccc
     1681 ttactccgaa cggccaacgc gtccgtcccg cacattccag caacggatct ggaggtaatg
     1741 tgcgggtatt acttcgacct gttctggtac cggaactcgg tctggttatc gttaagccag
     1801 gccgtgaatc aatgtcagca ttccataacg gcagaatact ggtggagccg gaaccaaaaa
     1861 gcatgcgtaa tctgccgtcc ggggtcgttc ctgccgctcg ccagccgctg gtggaagaca
     1921 aaacattgct gccgtttttc agtaacgcac gggtgattcg tgctgctggt ggtgctggtg
     1981 cattgtctga ctggctgttg cgccatatta aatcctgcca gtggccacac ggcgattatc
     2041 atcacagcga aaccgtcatt caccgttatg gtaccggcgc aatggtgttg tgctggcact
     2101 gcgacaacca gctgcgtgac cagacatccg aatcactcga gcaacttgct catcaaaacc
     2161 tgtcagcatg gatgattgac gtcatcggtc acgcaataag cggtacgcag gagcgtgaat
     2221 tatctctggc tgaattatcc tggtgggcgg tccgcaatca ggtggcggac gcgctaccgg
     2281 aagcggtatt acgtcgttcg ctggggttgc gtgcggaaaa aattcgctct gtgtacagtg
     2341 aaagcgacat cataccggga gagcagacag ccaccagcat actgaagcag cgcacaaaaa
     2401 atattgcgtt actgcctcac gtccaccagc aacagaaccc accacaggaa aagacggtgg
     2461 tcagcattgc cgttgatccg gagtcaccgg ctcagtatct ccagcgccag aaatcacaac
     2521 gggaagagat gcctgtatac acgcgctggg taaaaacgca gaaatgcatg acgtgtggca
     2581 atcaggcaga tgatccgcat cacatcattg gtcatggact gggagggatg ggaacaaagg
     2641 ctgatgattt gtttgttatt ccgttatgcc gtaaatgccg taacgaacta catgccggag
     2701 taaaagattt tgaagagaaa cacggcagtc agctgttgtt gctgattcgt tttttaatgc
     2761 acgcgagaaa ttcgggtgtt ctgaagtgga aagcataaat gaccgagcgc atagaatttg
     2821 ttttacctta cccgccgacg gtgaacactt actggcgtcg tcgtggcagc acatattttg
     2881 tatcaaaagc cggggagcgt tatcgccggg cagtggcgct tattgttcgc cagcagcggc
     2941 tgaaattaag cctgtccgga aggttggcaa taaaaattat tgcagaacca ccggataagc
     3001 gccgccgtga cctggacaat attctgaaag cgccgctgga tgcgctgacg catgcggggt
     3061 tgctaatgga cgatgagcag tttgatgaaa tcaatatcgt tcgtgctcag ccagtatctg
     3121 gtggacgtct gggggtgaag atttatccca taatgcttga agggcaggtc aaaaaatgaa
     3181 actggaagat ttaccgaaat actactcccc aaaatccccc ggcctgactg atgcatcggc
     3241 ctcaacgtcg aaagatgcgc tgagtatcac tgatgtgatg gccgcgcagg gcatgacaca
     3301 gaatcgggct gagatggggg tttctgcgtt ccttgggaaa atgggcatta gtatgaatga
     3361 cagagagcgg gcaacagaat tgctgacaga atatgcactc agtcggtgtg atcgcgtggc
     3421 ggcgttaaga aaactcccgg cagaaataaa accggcagtg atgcgtatta tggcttcgta
     3481 tgcgtttgaa gattatgccc gtagcgcggc gagcaaaaaa cagtgcccct gctgtcacgg
     3541 aaaaaaattt attgaaagcg aggtttttac aaacaag
//
LOCUS       DADMKF010000259         3568 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.264, whole genome shotgun sequence.
ACCESSION   DADMKF010000259 DADMKF010000000
VERSION     DADMKF010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3568
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.264"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(60..1457)
                     /gene="ccp"
                     /locus_tag="JNC24_004025"
     CDS             complement(60..1457)
                     /gene="ccp"
                     /locus_tag="JNC24_004025"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HAY9160640.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHALNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLGVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            1862..3511
                     /gene="treF"
                     /locus_tag="JNC24_004026"
     CDS             1862..3511
                     /gene="treF"
                     /locus_tag="JNC24_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase TreF"
                     /protein_id="HAY9160641.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKMI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
BASE COUNT          901 a          927 c          851 g          889 t
ORIGIN      
        1 cagactttat tatcgtaata aaaatcgccc aaacattcct gttcgggcga tcaaattaat
       61 tattgtttat cctgcatata cggcgtgtac accccgttca gactgtgcag gaaagctacg
      121 atatcatcca catcctcctg cggcagctct ttgcctacct gatagcgcag catcagtttc
      181 accgccccgt ccagcgtcgg cacgtcaccg cgatggaagt acggtgccgt taaagcaacg
      241 ttacgtaaac cgggtacttt ctgacgcaat ttatcacgct cttctttagt aacattcata
      301 cgaccaatat ccgccgccgt aatttcccca aagttaaagt cttttttcag ccccaacggt
      361 tcaaaggaac gtccaccgag aataatacca ccatgacaag ttgcacattt attatcttta
      421 aataattgat agcctttttt ctgttgcgcc gtcagcgcat tttcatctcc acgcaaccat
      481 ttatcaaatg gggaatccgg cgtaattaat gttttctcaa attcagcaat ggcatcagta
      541 atattttcgc cactgaaacc ttgcggatag acgccgagga actgcgcttt aagctgcgga
      601 tctttttcca gcttagcaat aatttcgtcc caagatttcg acgccatttc aatcgggttc
      661 aacggcggtc caccagcctg atcctgcaat gttgccgcac gaccatccca gaactgctca
      721 acgttaaata ctgagttaaa taccgtcggc gcgttaatcg gcccaactgc gccaccaaca
      781 ccaatcgatg tttttctgcc atcgacgccc cccgcattca acgcatggca atgagcgcat
      841 gaaatggtgc tatcagccga taaacgggga tcgtgataca gcgcaaagcc caacgccact
      901 ttttgcgcat cggtaggcag tttttgcggg atgggctgca ccggttcatt gcgatgctcc
      961 ggagcagtat cattgctggc gtaatattcc gcgcgctgtt ttgcaatcca ggccagtatt
     1021 tccgcccgct cttcatcact caccttaccc gcccagtgaa gcgcggtata acgcgttggc
     1081 ggcatagttt catactgcat cacccattca atcttattca gatcgctttg cgaaacgggt
     1141 ttatcagcca gcagtgccgc acgcacggcc tcgaggttaa aagatttata tccaagctta
     1201 atgtcgtaat ccatcaactg tttcgcgcca ggaatataat aataggcggg taattctgcc
     1261 gaaggcgtgt ggcaatagtc gcatcctttt tcgcggagaa aacctaaaac cttattattt
     1321 tcactgacag cggatgcctg aacatcggcc tgtttactgc gtttattatc gtggtaccac
     1381 acataaccag ataaccctaa atagcaaatc gcgacgccag ccaggccgat cgcggtaata
     1441 cgtgagacca ttttcattat tcttccctca cggtggtgag ttattgtgac ccagaaaatt
     1501 ttccagaatg ctgcaaacgt ctctgcgaga caaagccatc ataaaaaaca gtgagatacg
     1561 ctttttgata ggaatcaatc aattacatga atataaacta tcaataagat agcctgtgcc
     1621 tattacccag atcaggaatg gcatatcatg attgcggcga gagcaggatt gaatgttctg
     1681 aaaatgaaaa acaacacgaa atccgtattt ttataaattc catggatatt agtgcgttat
     1741 attttttgac aggttaataa acgtgatcca ccgcacgctt tgtcgcccac caggcggagc
     1801 gaatgactac ccttaaagaa aagcccgata attagcgacg aatttcggag gttggatcct
     1861 tatgctcaat cagaaaattc aaaaccctaa tccagacgaa ctgatgatcg aagtcgatct
     1921 ctgctatgag ctggacccgt atgaattaaa actggatgag atgatcgagg cagaaccgga
     1981 acccgagatg attgaagggc tgcccgcctc tgatgcgctg acgcctgccg atcgctatct
     2041 cgaactgttc gagcatgttc agtcggcgaa aattttcccg gacagtaaaa cctttcccga
     2101 ctgcgcacct aaaatggacc cgctggatat tttaatccgc taccgtaaag tgcgccgtca
     2161 tcgtgatttt gacttgcgca agtttgttga aaatcacttc tggctgccgg aggtctactc
     2221 cagcgagtat gtatcggacc cgcaaaattc cctgaaagag catatcgacc agctgtggcc
     2281 ggtgctaacc cgcgaaccac aggatcacat tccgtggtct tctctactgg cgctgccgca
     2341 gtcatatatt gtcccaggcg gccgttttag cgaaacctac tattgggact cctatttcac
     2401 catgctgggg ctggcggaaa gtggtcggga agatttgctg aaatgcatgg ccgataactt
     2461 cgcctggatg atcgaaaact atggtcacat ccccaacggc aaccgcacct attatttgag
     2521 ccgatcgcaa ccaccggttt ttgcgctgat ggtggagttg tttgaagaag atggtgtacg
     2581 cggtgcgcgc cgctatctcg accaccttaa aatggaatat gccttctgga tggacggtgc
     2641 agaatcgttg atccctaatc aggcctatcg ccatgttgtg cggatgccgg acggatcgct
     2701 gctcaaccgt tactgggacg atcgcgacac gccgcgtgac gaatcctggc ttgaggacgt
     2761 tgaaaccgcg aaacattctg gtcgcccgcc caacgaggtg taccgcgatt tacgcgcggg
     2821 ggcggcctcc ggttgggatt actcttcccg ttggctgcgt gatactggtc gtctggcgag
     2881 cattcgtacc acccagttca tccccatcga tctgaatgcc ttcctgttta aactggagag
     2941 cgccatcgcc aacatctcgg cgctgaaagg cgagaaagag acagaagcgc tgttccgcca
     3001 gaaggccagt gcccgtcgcg atgcggtaaa ccgttacctc tgggatgatg aaaacggcat
     3061 ctaccgcgat tacgactggc gacgcgaaca actggcgctg ttttccgctg ccgccattgt
     3121 gccgctctat gtcggcatgg cgaaccatga acaggccgat cgtctggcaa acgccgtacg
     3181 cagccggtta ctgacacctg gcgggattct ggcaagcgag tacgaaaccg gtgaacagtg
     3241 ggataaaccc aacggctggg caccgttaca atggatggca attcagggat ttaaaatgta
     3301 tggcgatgac cttctgggtg atgaaatcgc gcgcagctgg ctgaaaacgg tgaatcagtt
     3361 ctatctggaa cagcacaaaa tgatcgagaa ataccatatt gccgatggtg ttccccgcga
     3421 aggcggcggt ggcgagtatc cgttgcagga tgggtttggc tggactaacg gtgtggtacg
     3481 ccgtttaatt ggtttgtacg gcgaaccata atatttttac agccagccgc taacttcctg
     3541 ctggctgtaa aattatcctc ttcaggag
//
LOCUS       DADMKF010000260         3546 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.265, whole genome shotgun sequence.
ACCESSION   DADMKF010000260 DADMKF010000000
VERSION     DADMKF010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3546
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.265"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(<1..143)
                     /locus_tag="JNC24_004027"
     CDS             complement(<1..143)
                     /locus_tag="JNC24_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9160642.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVL"
     gene            complement(189..530)
                     /locus_tag="JNC24_004028"
     CDS             complement(189..530)
                     /locus_tag="JNC24_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9160643.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            complement(699..890)
                     /locus_tag="JNC24_004029"
     CDS             complement(699..890)
                     /locus_tag="JNC24_004029"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9160644.1"
                     /translation="MGPALGAARLAQIAANPEKSLIELLPQLPLEQSHLPDAQRYAAY
                     QPRRETFRRLYQQLLPLMA"
     gene            complement(823..2154)
                     /gene="xylB"
                     /locus_tag="JNC24_004030"
     CDS             complement(823..2154)
                     /gene="xylB"
                     /locus_tag="JNC24_004030"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005054141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HAY9160645.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVDSQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWAAKLTGLCNVPALIAAAQQADESVEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQKLDYRTGGGCGASTGRSKAGADCGESREIAH"
     gene            complement(2226..>3546)
                     /gene="xylA"
                     /locus_tag="JNC24_004031"
     CDS             complement(2226..>3546)
                     /gene="xylA"
                     /locus_tag="JNC24_004031"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HAY9160646.1"
                     /translation="QAYFDQLDRVRYEGSKSSNPLAFRHYNPDELVLGKRMEEHLRFA
                     ACYWHTFCWNGADMFGVGAFNRPWQQPGEALALAKRKADVAFEFFHKLHVPFYCFHDV
                     DVSPEGASLKEYINNFAQMVDVLAAKQEESGVKLLWGTANCFTNPRYGAGAATNPDPE
                     VFSWAATQVVTAMEATHKLGGENYVLWGGREGYETLLNTDLRQEREQLGRFMQMVVEH
                     KHKIGFQGTLLIEPKPQEPTKHQYDYDAATVYGFLKQFGLEKEIKLNIEANHATLAGH
                     SFHHEIATAIALGLFGSVDANRGDAQLGWDTDQFPNSVEENALVMYEILKAGGFTTGG
                     LNFDAKVRRQSTDKYDLFYGHIGAMDTMALALKIAARMIEDGELDKRIAQRYSGWNSE
                     LGQQILKGQMSLADLAKYAQEHNLSPVHQSGRQEQLENLVNHYLFDK"
BASE COUNT          924 a          983 c          784 g          855 t
ORIGIN      
        1 agtacggcaa aataataccc tttttcattt aactgcatct ctgcattcca tagccctaac
       61 agataggtaa cgataccacc aacgagagct atccatgaca caatactaaa ggccggtgaa
      121 taggttgata ttttgttgtc catcacagta ttcctttcat tcctgaatgt gtaagagctt
      181 tatgttgctc ataccgagcg aaacaaacgc atttgaccaa acaagcagac aaaaaaggcg
      241 atggggtaga tcaccatttc ataagcattg ataggtgcat ttaaaacacc gataaacaag
      301 agaccgatag tgattaacgc aactaactgg cagatatggg taaaaaaatt atccagttgc
      361 ccgagttttt ctgcgcgaag atatgcatag ttaccaaaag ttgctgtcat taacacgcca
      421 agaaaatagc cttttccact tagcaatggg catgagatcc atagcccaac cagataaacc
      481 agcgccccga caacaaataa tagttttgcc acagtttttg aggttttcat aacgatctcc
      541 atatctacca gcgatacatt acgagtaacc aacgaaagac aaaactgaag aatgccatta
      601 acaaatgatt ttcagaataa attcatacta aatattaatt aattagtgag atatatagat
      661 gtgaattatc ccccacccgg tcaggcaggg gataacgttt acgccattaa tggcagaagt
      721 tgctgataga ggcgacggaa cgtttcacgt cgtggctgat aagcggcata acgctgcgca
      781 tctggtagat gcgactgttc taacggtagc tgcggcaaca actcaatgag cgatttctct
      841 ggattcgccg caatctgcgc cagccttgct gcgcccagtg ctggccccac atcccccccc
      901 cgtacggtaa tcgagcttct gaccgctgat atccgccagc atctgacgcc agtactcact
      961 acgcgccccg ccaccaatca acgtaacact ttgcggttta atgccgcagg catgcacgac
     1021 atccatgcca tctgccagcg cataacccac gccttccagc actgctcgcg ccagttcatt
     1081 ggggccatgt tgatgagtca aaccaaagaa aacccccttc gcctggggat tattgtgtgg
     1141 tgtacgctcg ccggaaagat aaggcagaaa ccaaactggc tcgacacttt catcagcctg
     1201 ttgagctgca gcgattaaag ctgggacatt gcacaggccg gttaatttcg cggcccaatc
     1261 cagacacgat gctgcactca gcattacaga cattaaatgc caacgctgcg gtagcgcatg
     1321 gcaaaagctg tgtacagcgc tttctggctt gcttaagaac ccttcgctga cagcaaaata
     1381 gacccccgac gtccccagcg ataacattgc ctgattagca tcaaccattc ccacaccaac
     1441 tgcaccagct gcattgtcgc caccgcctgc gacaactggc accgtcgcca taccccacgc
     1501 tttcgcaact tcaggtaaca aagcaccagt aatttcgctg ccttcgtata atgcgggcat
     1561 ctggtcacga gataagtcgc aagcctgcag catgacgtca ctccagtcac gctttgcgac
     1621 atccagccac atggtgccag ctgcgtcaga catatcgctg gcaaactccc ccgtcatacg
     1681 cagacgcaag taatctttcg gtaataatac tttgtcgatt tgacggaata tctccggctc
     1741 atgccgctga acccatagca atttaggcgc agtaaatccg ggcatcatca ggttgccggt
     1801 aatcactcgt gattgcggaa ctctcgcttc cagcaaagtg cactcttgcg cacagcgccc
     1861 gtcgttccac aaaatggcag ggcgcaatac ccgttgttga gcatccagta aggttgctcc
     1921 atgcatctgg ccggcaatac ccaatgcttt aacgtcctgc agagaatgct gatcgcccag
     1981 agctttcatt gcgcgatcag ttgcctgcca ccactgttcc gggtcttgtt ccgaccagag
     2041 tggatgcggg cgcgaaacgg tcagcttttc cgtttgcgaa tcaaccacct caccctgctc
     2101 gttgagcaaa ataactttta cgcccgaggt gccaagatct atcccgatat acatatcgat
     2161 cgttccttaa aaaaatgccc ggtatcgcta ccgataaccg ggccaacgga ctgcacagtt
     2221 agccgttatt tgtcgaacag ataatgattt accagatttt ccagttgctc ctggcgacca
     2281 ctctgatgca ccggagacaa attatgttcc tgagcatatt tggctaaatc tgccagtgac
     2341 atttggcctt tcaggatttg ctggcccaat tcgctattcc agccggaata acgctgcgcg
     2401 atgcgtttat ccagctcgcc atcttcaatc atgcgcgctg caattttcag cgccagtgcc
     2461 atcgtatcca tcgcgccgat atgaccgtaa aacagatcat atttatcagt actttgacga
     2521 cgtactttgg catcgaagtt cagaccaccg gtggtgaaac cgcctgcttt gagaatttca
     2581 tacatcacca gcgcattctc ttccacactg ttcgggaact ggtcggtgtc ccagcccagt
     2641 tgcgcatcgc cacggttggc gtcgacagaa ccgaacaggc caagcgcaat ggcggtggct
     2701 atttcatgat ggaaagagtg acctgccagc gtcgcgtggt tagcttcaat gttcagttta
     2761 atctcttttt ccagaccaaa ctgtttcagg aagccataga ccgtcgcggc atcgtaatca
     2821 tattgatgtt tagtcggttc ttgcggtttc ggttcgataa gcaacgtgcc ctggaagccg
     2881 attttatgtt tatgctcaac caccatctgc ataaagcggc ccagttgttc acgctcctga
     2941 cgcaagtcgg tatttaacag cgtttcgtaa ccttcacgac caccccacag gacatagttt
     3001 tcaccgccca atttatgggt tgcttccatc gctgtaacaa cttgcgttgc cgcccagctg
     3061 aagacttcag gatctgggtt cgtcgccgca cccgcgccat agcgagggtt tgtaaagcag
     3121 ttagcggttc cccacagcag cttcacgccg ctctcttctt gcttggctgc caggacatca
     3181 accatttgcg caaaattatt gatgtactct tttaacgacg cgccctcagg ggaaacatcc
     3241 acatcgtgga agcaataaaa tggcacatgt aacttgtgga aaaactcaaa tgcgacatct
     3301 gctttacgct tcgccaacgc cagtgcctca ccaggctgct gccacggacg attaaacgcc
     3361 cccacaccaa acatgtccgc cccgttccag cagaaggtgt gccagtagca ggcggcaaaa
     3421 cgcaagtgct cttccatacg cttacccaac accagttcgt cgggattgta gtgacggaat
     3481 gctaacgggt ttgaggattt tgagccttca taacgaacgc gatcgagctg gtcaaaatag
     3541 gcttgc
//
LOCUS       DADMKF010000261         3507 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.266, whole genome shotgun sequence.
ACCESSION   DADMKF010000261 DADMKF010000000
VERSION     DADMKF010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3507
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.266"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(<1..1523)
                     /locus_tag="JNC24_004032"
     CDS             complement(<1..1523)
                     /locus_tag="JNC24_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HAY9160647.1"
                     /translation="MKKAPNLKHQPRDKMTEVIIFAGNDAWAHAKQWQEQDGRLAGDS
                     VPPVWLGEQQLAELDKLQIVPDGRYRVRLYQAGLLRPGLVNTIGQKLAAAGVRDADYY
                     PEGMHSQKRENWREYLERERAEQAEKKKVVELPVKKKERVKDDNASSLALNQMGASQR
                     GEVLLAHYGGELAIHADSDTVHHYNGVVWEPVQDKELQRAMAQIFIDAEISYSQNAIK
                     SAVDTMKLSLPVMGNTARNLIGFSNGVFDTRTGNFREHNKNDWLLIASELPFSPPAEG
                     ETLATHAPNFWKWLRRSVAENDRKADRVLAVLFMVLANRYDWQLFIEVTGPGGSGKSV
                     MAEICTMLAGKANTVSASMKALEDARERALVVGFSLIIMPDMTRYAGDGAGIKAITGG
                     DKVAIDPKHKAPYSTRIPAVVLAVNNNAMSFSDRSGGISRRRVIFNFSEVVPENERDP
                     MLAEKIEGELAVVIRHLLTRFSDQDEAKRLLYEQQKSEEALVIKREGDSLVDFCGYL"
     gene            complement(1520..1819)
                     /locus_tag="JNC24_004033"
     CDS             complement(1520..1819)
                     /locus_tag="JNC24_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HAY9160648.1"
                     /translation="MRTYLSGLTASGYAHPKIIPGAIYLDKNGNRVTVKELMFDRVYF
                     IRDGYSFHSSLNVEIFISRFRREIPLSRNNHVSCMDVDKKLQELKNMIAAWREQK"
     gene            complement(1826..2146)
                     /locus_tag="JNC24_004034"
     CDS             complement(1826..2146)
                     /locus_tag="JNC24_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001113153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9160649.1"
                     /translation="MPDMTNYQYLINPHFNCEHDIAKKVYSAADGATDNISMAVASIG
                     SLMWHASENEEYDEKAMRIDMGNIGLLLAMLGQFDISLRCTIENATDALNAIEKANTD
                     SNLR"
     gene            complement(2139..2336)
                     /locus_tag="JNC24_004035"
     CDS             complement(2139..2336)
                     /locus_tag="JNC24_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HAY9160650.1"
                     /translation="MVAVNHIPHLVHTQTAFVWRFLALSAGESQIIHVTAWTEREARN
                     RCPSGCVAVFAARIRQEVCHA"
     gene            2390..2842
                     /locus_tag="JNC24_004036"
     CDS             2390..2842
                     /locus_tag="JNC24_004036"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9160651.1"
                     /translation="MRGFVVSIAELHVSALVVFTLHLGEYHFKSFLVFTFVLHCIPLL
                     MGGANMSRSSVNNKSQQLNARFPHEVVSGIEASLQPGETKANFIVTAVRGEIARRQAE
                     GSGENPLVSSLDALANVEQIGIKAAEEIGQLITVAREELQRRKAKESE"
     gene            2900..3076
                     /locus_tag="JNC24_004037"
     CDS             2900..3076
                     /locus_tag="JNC24_004037"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9160652.1"
                     /translation="MTNEESTKKTSPARKRRRRKKEHESERFAPCSFALEKYLKEYSS
                     KERASQLWQRTETD"
     gene            complement(3188..3397)
                     /locus_tag="JNC24_004038"
     CDS             complement(3188..3397)
                     /locus_tag="JNC24_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family phage regulatory protein"
                     /protein_id="HAY9160653.1"
                     /translation="MREINEDRVIREDECRKLTGVCRTTRYELEKKGRFPSRLNLGGR
                     SVGWLLSEVMEWVKSRDRINSGKAV"
BASE COUNT          881 a          939 c          744 g          943 t
ORIGIN      
        1 atgagatagc cgcagaagtc caccagcgaa tcgccctcgc gttttatcac cagagcttct
       61 tctgatttct gctgctcata cagcaggcgt ttagcttcgt cctggtcaga aaaacgagta
      121 agcaggtggc gaatcacaac cgccagctct ccttctattt tttctgccag catggggtcg
      181 cgttcgttct ccggtacaac ttccgaaaaa ttgaatatca cccgacgacg tgagatcccc
      241 ccgctgcggt cactgaatga catggcgtta ttgttaaccg ccagcactac tgccggaata
      301 cgcgttgagt agggggcttt gtgtttcggg tcaattgcca ccttgtcacc gcctgtaatg
      361 gccttaatcc ctgccccatc accagcgtag cgggtcatat ccggcatgat aatcagcgaa
      421 aagccaacca ctaacgcgcg ttcccttgca tcttccagcg ccttcatgct tgccgatact
      481 gtgttggcct tacccgccag catggtgcaa atctccgcca tcacgctttt accacttccc
      541 cctggacctg ttacctcaat gaataactgc cagtcgtacc ggttcgccag caccatgaat
      601 aatacagcca gtacgcgatc cgccttgcgg tcattctcag ccaccgaacg gcgtaaccac
      661 ttccagaaat tcggcgcatg tgttgccagc gtttccccct ctgctggtgg gctgaaaggt
      721 aattcactgg caattaacaa ccagtcgttt ttgttatgct cccgaaaatt acctgttctg
      781 gtatcaaata ccccgttact gaatccaatc aggttacggg ctgtattccc cattacaggc
      841 aaacttaact tcatggtatc gaccgccgat ttaatggcgt tctgcgaata gctgatctcc
      901 gcatcaatga aaatctgtgc catagctcgc tgtaattctt tatcctgtac tggctcccat
      961 acaacgccgt tgtaatggtg aacagtgtca gagtcagcat gaatcgccag ttcaccgcca
     1021 taatgtgcca ggagaacttc gccgcgttga cttgctccca tctggttaag cgccagtgat
     1081 gaagcgttat cgtcttttac ccgctctttt ttctttacag gcagttcaac tacctttttc
     1141 ttttccgcct gctctgcccg ttcacgttcc agatattcgc gccagttctc ccgtttctgg
     1201 ctgtgcattc cttcagggta ataatcagca tccctgacac ctgccgctgc cagtttctgc
     1261 ccgatggtat taacaagccc cggacgcaat aaccccgcct ggtagagacg cacccgatag
     1321 cgtccgtccg gtacgatttg cagcttatcc agttcggcaa gttgttgttc tccaagccag
     1381 acaggaggca cgctgtcgcc agccagtcgc ccgtcctgtt cctgccactg cttcgcatgt
     1441 gcccacgcat cattacccgc aaaaatgatg acttccgtca ttttgtcacg cggctggtgt
     1501 tttaaatttg gcgctttttt catttctgct ctctccacgc ggcaatcatg tttttcagtt
     1561 cctgtagttt tttatcaaca tccatacatg acacatggtt atttctggaa agcgggattt
     1621 cccgcctgaa tctgctaata aagatctcca cgttcagcga actatgaaat gaatagccat
     1681 cacgaataaa atacacacgg tcaaacatca gttcttttac cgttactctg ttaccgttct
     1741 tatccagata aatagcgccg gggataattt tggggtgtgc ataaccgctg gcagtcaagc
     1801 cagataaata cgttctcatg attatttatc tcagatttga atcagtattc gctttctcta
     1861 tggcatttaa tgcatctgtg gcattttcaa tggtgcaccg taacgaaata tcaaactgcc
     1921 caagcattgc cagtaacaag ccaatattac ccatgtcaat gcgcatagcc ttttcgtcat
     1981 attcctcatt ttctgacgca tgccacatca ggctaccaat tgacgcaaca gccattgata
     2041 tattgtcagt agccccatcc gcagcggaat aaaccttttt agcaatatca tgctcacagt
     2101 taaaatgcgg attaatcagg tactggtaat tggtcatgtc aggcatggca cacctcctga
     2161 cgaatacggg cggcgaatac agcaacacaa cctgacgggc aacggttacg cgcttcgcgt
     2221 tccgtccagg cggttacgtg gatgatttga gattctccgg cactcagggc cagaaaacgc
     2281 cacacaaagg ccgtttgtgt gtgtacaagg tgtggtatat gatttacggc aaccataacg
     2341 gctcctcgtt tacgttgttg gttagaagcc ctgcgagtgg taacgacact tgcggggctt
     2401 cgtcgtttca attgctgaat tgcatgtatc agcacttgtg gtattcacat tacatttagg
     2461 tgaataccat ttcaagtctt ttttggtatt cacttttgta ttacactgca tcccgttatt
     2521 aatgggaggt gcaaacatgt ccaggagttc tgttaacaat aagtcacagc aactgaatgc
     2581 cagatttcca catgaagtag tgagtggcat tgaggcatcc ctacagccag gggaaactaa
     2641 agcgaatttt atagttacgg ctgtacgcgg tgagatcgcc aggcgccagg cagaaggaag
     2701 tggagaaaat ccccttgtgt cttcactgga tgccctagct aatgtcgaac aaatcggcat
     2761 caaggcagcg gaggaaatcg ggcagcttat caccgttgca cgcgaagaac tccagcgccg
     2821 caaggccaaa gaatcagaat aatcactatc agcgccgtgg tgtgaggaac tccggcgcat
     2881 tgctttacag gcgcacacaa tgaccaacga agaatcaacc aaaaaaacat cacctgcacg
     2941 aaaaagacga cgcagaaaga aagagcatga atcagaacga ttcgcgccgt gttcttttgc
     3001 ccttgagaag taccttaaag aatactcatc aaaggagaga gcttcgcagt tatggcaacg
     3061 cactgaaaca gactgatagc attgcccacc agcctgatag cggctatcat tgacatgctt
     3121 atgtttagtg tttccaaact ggcgaccgcc ccaggtcgcc tttgttttat gtgtcatatg
     3181 ctccccttta cactgcctta cctgaattaa tgcgatcccg gcttttaacc cattccataa
     3241 cctcggacag cagccaccct acagaacgac cgcccagatt aagacgtgac ggaaagcgcc
     3301 cttttttctc cagttcgtaa cgtgtagtgc ggcacactcc agttaactta cgacattcat
     3361 cctcacggat tacgcgatct tcatttattt cacgcataca aaaacctaca taaaaattac
     3421 gtatataaac ttttctctta gctgtagata tatgagatcg gatattactt agattctttt
     3481 tcacctctta aattaaaaac acaacca
//
LOCUS       DADMKF010000262         3397 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.267, whole genome shotgun sequence.
ACCESSION   DADMKF010000262 DADMKF010000000
VERSION     DADMKF010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3397
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.267"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            89..403
                     /locus_tag="JNC24_004039"
     CDS             89..403
                     /locus_tag="JNC24_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001301570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9160654.1"
                     /translation="MYNKKNKTGTVALSGTWQQGNRESKSIRRNIEYTWIENYDTAHL
                     TSKKVNKFEIMDQVDDDRLAQLIPDFYVFPEKSVSYNILKQGKHAFILSIGNRAIMHC
                     AR"
     gene            complement(553..977)
                     /locus_tag="JNC24_004040"
                     /pseudo
     CDS             complement(553..977)
                     /locus_tag="JNC24_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417326.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1407..1897
                     /locus_tag="JNC24_004041"
                     /pseudo
     CDS             1407..1897
                     /locus_tag="JNC24_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311734.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2122..2613
                     /locus_tag="JNC24_004042"
     CDS             2122..2613
                     /locus_tag="JNC24_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAY9160655.1"
                     /translation="MSTETIEIFNNSDEWANQLKHALSKGENLALLHGLTPDILDRIY
                     AYAFDYHEKGNITDAEIYYKFLCIYAFENHEYLKDFASVCQPKKKYQQAYDLYKLSYN
                     YSPYDDYSVIYRMGQCQIGAKNIDNAMQCFYHIINNCEDDSVKSKAQAYIELLNDNSE
                     DNG"
     gene            2948..>3397
                     /gene="ygeH"
                     /locus_tag="JNC24_004043"
     CDS             2948..>3397
                     /gene="ygeH"
                     /locus_tag="JNC24_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HilA family transcriptional regulator YgeH"
                     /protein_id="HAY9160656.1"
                     /translation="MDLENKFSYHFLEGLTLTEDGILTQGNEQVYIPQKELGVLIVLL
                     ESAGHVVLKDMIIESVWKNIIVSDESLTRCIYSLRCIFEKIGYDRCIETIYRKGYRFS
                     GQVFKTKINEDNTSDYSIAIFPFTTSLNTLDPLILNQELVQIISNKKI"
BASE COUNT         1287 a          532 c          537 g         1041 t
ORIGIN      
        1 tgcagaaaaa atgatatata acgaaacatg cactgcaaat tgggtaattt ttaatgacca
       61 gggacgagca aatcttacca tcgactttat gtataacaaa aaaaataaaa caggaactgt
      121 tgcgttgagt ggaacctggc aacaaggtaa cagagaaagt aaatcaatac ggcgaaatat
      181 tgaatacaca tggattgaaa actatgacac agcccattta acatcaaaga aagttaataa
      241 gtttgagatt atggatcaag ttgatgatga tagacttgca caacttattc ctgattttta
      301 tgtctttcca gaaaaaagtg taagctataa tatactaaag caaggtaagc atgcttttat
      361 tttgagcatt ggtaacagag caataatgca ttgtgcaagg taaactaaaa tagataacaa
      421 aaaagatgga acctcgttaa tcataaaaca ataaaattaa atattctcgc agtatatggc
      481 agtctaaagc atcaaagttt tgatcaacat ctttcatttt agacatctcc ttgcaatgta
      541 aaatatcatg tatcaacaac atctggtgca aatccatgag tcgaactcga cattcatctt
      601 agctcgatta ttattccttc gtacagtcga tgtaaacaat acagaaagag gattattaag
      661 aaccagttta ctcaggaatc aatagaggaa aggactaacg tttctttaaa agaattgatt
      721 tcatcatctg ttaaactaaa ctcatcattg acagatcgtg agatataact gtttttaact
      781 ttactcttta cgttgacttt attgacagaa ttaacattca catatcttga atttaatgtc
      841 catgatgttg tttgcgtgag aacattctca gcattaagga atttttttaa cggaatcctt
      901 ggtttctttt tcgagtccga atttacaata tcatgcatat agaccatttc atgaatctgc
      961 gaaaactcaa tgtccattgt atacctcaca tttttaccgt gactcgatgt tactgttcaa
     1021 taatcacctt ccatcaatac taaaattaat acccctaatg tgccgataac aaatatagtc
     1081 attctacgta acgtctccat aaggtgataa ccttgatcaa atgaatttcg aatataataa
     1141 aggaaatctt ttgagatgtt tatcacgaat aaagctattt atgttaccaa tatcatttcg
     1201 agtgcgcaca ctatctatct gttttttcga gatataaaaa acaacgtgta agttatgtaa
     1261 attgccgatg ctactgagta acattatggt aaaaaaagta aaaatgagcg atttttgata
     1321 gaataaagca tgagaaaagg tgatatttga cattatcaga agctgcgaat tcggattttg
     1381 ctctaatcaa atgagagaaa tgtaaaatga agccacgaaa tattaataat agcctaccac
     1441 tgcaaccatt agttcctgat caggagaaca aaaataagaa aaatgaagag aaatccgtta
     1501 atccagttaa aatcacaatg gggtctggtt taaattatat tgaacaagaa tctcttggag
     1561 gaaaatatct aacacatgat ttgtcaataa agatagcgga tatttctgaa gagataattc
     1621 agcaagcaat attatctgct atgagcatat ataaattttc gataacagat gatttaatga
     1681 gtatggctgt aaatgaactc ataaaactga ccaaaataga gaataatgta gacctgaata
     1741 aattcactac tatatgcaca gacgttctat cccccctcgt caccagacat aataaagaaa
     1801 aaaaacaaac gacattctac ccttctcaaa aatccccttt ttaattttca ttgaaaaaat
     1861 ggcaattgat actatgaatc caggtcatac acattaaggc atttatgtaa tgtcgtaatt
     1921 aatataacta ataaggtgaa tattagtaaa acaactaatg atgttattag tttgtagtga
     1981 acagtacttt taccaataat gaaaaatata ccataagcaa tgcaagctta aaactaataa
     2041 tttaaaaaag ataaatgtaa aaatattaat gagtcaatca ataataattt agcatcacga
     2101 gacacatcac agaggaatat tatgagcaca gaaacaattg aaatattcaa taatagtgat
     2161 gaatgggcaa atcaactaaa acacgcatta tcgaaaggag aaaatctggc attactacat
     2221 ggtttaactc ctgatatcct tgatagaata tatgcatatg cattcgacta ccatgaaaag
     2281 ggtaatataa cggacgcaga aatttattat aaatttttgt gcatttatgc gttcgaaaat
     2341 catgagtatc taaaagattt tgcatcagta tgtcagccaa aaaagaaata tcaacaagca
     2401 tatgaccttt acaaactaag ttacaattac tccccgtatg atgactattc agttatttat
     2461 cgtatgggtc aatgtcagat tggggctaaa aatatcgata acgcaatgca atgtttctat
     2521 cacattatta acaattgtga ggatgatagt gttaagagta aagcgcaggc atatattgaa
     2581 ctcttaaacg ataattcaga agataatggc taagcattac aatcatttgg ttaaatttgc
     2641 tggttatagt gcagttaact actataacca gcacctctta tgttttctta aatcagcatc
     2701 ttaaaacagt taagagcata ctataaatca ttatttttca atgcgttaga gaacaatcta
     2761 ctttataaaa attgaataag gctatttcag attacatcac tgaattccta agataaatta
     2821 gtgaacacaa ccatagatat gtcgaatgta ttttcctgta gcattaatga cacataaata
     2881 tgaatagcca taatttctat tgcttaagca ggatgcaaga aaccaatttt ttcatagagg
     2941 ttaactaatg gacttagaaa ataaattctc atatcatttt cttgagggat taacgctcac
     3001 ggaagatgga attcttactc aaggaaatga gcaagtttat attccacaga aagagttagg
     3061 tgtattaata gtattacttg aatccgctgg tcatgttgta ctgaaagata tgatcatcga
     3121 atcagtatgg aaaaatatta ttgttagtga cgagtccctg acaagatgta tctattcttt
     3181 gcgctgcatt tttgaaaaaa ttggctatga tcgttgcata gaaacaatct accggaaagg
     3241 ttatcgtttc agcgggcagg ttttcaaaac taaaattaat gaagataata cttcagacta
     3301 ttccatagct atattccctt tcactacttc attgaataca ctggatccat taatacttaa
     3361 tcaggaatta gtgcaaatca tttcaaataa aaaaatc
//
LOCUS       DADMKF010000263         3380 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.268, whole genome shotgun sequence.
ACCESSION   DADMKF010000263 DADMKF010000000
VERSION     DADMKF010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3380
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.268"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(377..452)
                     /locus_tag="JNC24_004044"
     tRNA            complement(377..452)
                     /locus_tag="JNC24_004044"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(417..419),aa:Asn,seq:gtt)
     gene            complement(553..2196)
                     /gene="yeeO"
                     /locus_tag="JNC24_004045"
     CDS             complement(553..2196)
                     /gene="yeeO"
                     /locus_tag="JNC24_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416491.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HAY9160657.1"
                     /translation="MLRHILTAKNLLSNPIFKFPNCLPFLSTVCCICRQFVGENLCSF
                     ADSPSLFEMWFHFLQLRSALNISSALRQVVHGTRWHAKRKSYKVLFWREITPLAVPIF
                     MENACVLLMGVLSTFLVSWLGKDAMAGVGLADSFNMVIMAFFAAIDLGTTVVVAFSLG
                     KRDRRRARVATRQSLVIMTLFAVLLATLIHHFGEQIIDFVAGDATTEVKALALTYLEL
                     TVLSYPAAAITLIGSGALRGAGNTKIPLLINGSLNILNIIISGILIYGLFSWPGLGFV
                     GAGLGLTISRYIGAVAILWVLAIGFNPALRISLKSYFKPLNFSIIWEVMGIGIPASVE
                     SVLFTSGRLLTQMFVAGMGTSVIAGNFIAFSIAALINLPGSALGSASTIITGRRLGVG
                     QIAQAEIQLRHVFWLSTLGLTAIAWLTAPFAGVMASFYTQDPQVKHVVVILIWLNALF
                     MPIWSASWVLPAGFKGARDARYAMWVSMLSMWGCRVVVGYVLGIMLGWGVVGVWMGMF
                     ADWAVRAVLFYWRMVTGRWLWKYPRSEPQKCEKKPVVSE"
     gene            2201..2276
                     /locus_tag="JNC24_004046"
     tRNA            2201..2276
                     /locus_tag="JNC24_004046"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2234..2236,aa:Asn,seq:gtt)
     gene            complement(2314..3264)
                     /gene="cbl"
                     /locus_tag="JNC24_004047"
     CDS             complement(2314..3264)
                     /gene="cbl"
                     /locus_tag="JNC24_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HAY9160658.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEL
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGTPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFRWHHSLLVPLDHPLTQITPLTLESIAKWPLITYRQGITGRSRIDDAFARKGLLA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEENLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
BASE COUNT          991 a          878 c          706 g          805 t
ORIGIN      
        1 actcgtcata ttgattatac ccccccgttc ccagagaaat aatatttatt aaaattccag
       61 ttcttctttt tctgattaca gaaggcaaag tggcaattac gcatagtttc ccgataaaga
      121 cgcgatagcg acatcccgca taaggcattt ttctctttat ctttgtacgg tacttcatgg
      181 aacagagttt ttgaccttgc gaatcgtgat gtctgttggg gaggggcaat ttgctcactg
      241 aagcgtgaga ctcgattaag cgcacgaaac acagaaatca aaaaacccgg tcactttttt
      301 acaaggtaac cgggtaaaaa taatttttat tttttaactg ttttgagact catagagatg
      361 tctcaaaact aaaatttggc tcctctgact ggactcgaac cagtgacata cggattaaca
      421 gtccgccgtt ctaccgactg aactacagag gaatcgtgag aacgaggcga atattagcga
      481 tgcccaccca caatgtcaaa gcctgttttt taaatttgaa atcgtttgct gaaataatct
      541 gcattttgtc gtttattccg acacaactgg ctttttttca cacttttgcg gttcggatcg
      601 agggtatttc cacagccaac gtccagtaac cattcgccag taaaacagca cggcccgcac
      661 agcccagtcg gcaaacattc ccatccagac accaaccaca ccccagccaa gcatgatgcc
      721 cagcacataa ccgacgacaa cccggcaacc ccacatgctc aacatagaaa cccacatggc
      781 gtaacgggca tcgcgagcac ctttaaatcc agcgggtagc acccatgagg cggaccaaat
      841 aggcataaat aaagcattta gccaaatcag aatcacaacg acatgtttaa cctgtggatc
      901 ctgggtgtaa aacgatgcca taaccccggc aaagggagcc gttagccagg cgatggccgt
      961 taatccaaga gtggaaagcc agaacacatg ccgcaactga atctctgctt gcgctatctg
     1021 ccctaccccc aaccttcggc ctgtaatgat cgtagaagca gagccgagcg cacttccggg
     1081 taagttgata agagccgcaa ttgaaaacgc gataaaattt ccggcaataa cactggtccc
     1141 catcccggca acgaacattt gggttaataa ccgaccactg gtaaataaca ctgattcgac
     1201 actcgcggga ataccaatcc ccatgacttc ccagataatg ctaaaattca gcggtttaaa
     1261 atagctcttt aacgaaatcc ttagcgcagg attaaaacca atcgccagca cccacaaaat
     1321 tgcaactgcg ccaatataac gagaaatggt taaacccagc cctgccccga caaatcccag
     1381 tcccggccag gagaaaaggc cgtaaatcaa tatgccgcta ataataatat taagaatatt
     1441 caggctaccg ttaatcaata gcggtatttt cgtattccct gcaccacgaa gtgccccgct
     1501 accaataaga gtgatggcag ctgctggata actgagtacc gtcagctcca gataagtcaa
     1561 cgccagtgct ttaacttctg tcgtggcatc acccgcgacg aaatcaataa tttgttcgcc
     1621 aaaatgatga ataagcgttg ccaacagtac ggcaaacaac gtcatgatca ccaatgactg
     1681 ccgcgtcgcc accctcgctc gtcgtcgatc ccgcttaccg agactaaatg ccacaacgac
     1741 agtagtacca agatcgatag cagcaaaaaa agccataatg accatattga agctgtccgc
     1801 caatcccacg ccggccatcg catcttttcc cagccagctg accagaaaag tgctcagaac
     1861 ccccatcaac aggacacagg cattctccat gaagatagga acagcaagcg gggttatctc
     1921 gcgccagaac aacactttgt agctcttgcg tttagcgtgc cagcgagtgc cgtgaacaac
     1981 ctggcgtaaa gcagaggaga tattcaaagc cgaccttaat tgcagaaagt gaaaccacat
     2041 ttcaaataat gagggagaat cagcaaagct gcaaagattt tcgccaacaa attgtctgca
     2101 aatgcaacaa actgttgata gaaacggcaa acagttgggg aatttaaaaa tcgggtttga
     2161 caaaagattt ttcgccgtta agatgtgcct caacagcgat tcctctgtag ttcagtcggt
     2221 agaacggcgg actgttaatc cgtatgtcac tggttcgagt ccagtcagag gagccaaatt
     2281 tctgaaaagc ccgcttttat agcgggcttt ttgctatatc tgataatcaa tttcctcttc
     2341 actgttttcc atcacctgcc gcttgatatc ctcaactgac agtcctgcat tacaaagttc
     2401 cagaaagcgc cagacatagt tacgctgaag ttgtcctcgc ttcagtccca accagacagt
     2461 attagcatcg aaaagatgcc gcgtatccag acggattaaa ttctcttcct cttgttcgcc
     2521 gctggattgt tcggcaacta atccgatccc aagcccaaga gcaacatagg ttttaatgac
     2581 atcagaatcc tgcgcactta atacaatatc tgccagcaaa cctttgcggg caaatgcgtc
     2641 atcaatacgt gagcgccccg taatcccctg tcggtaagtg attaacggcc acttcgctat
     2701 tgattccagc gtcaatggtg taatttgcgt taagggatga tcgagtggaa caagcaaact
     2761 atggtgccaa cgaaaccacg ggaaggcgac gagctgcggg tcattactca aacgctcgct
     2821 ggcgatacca atatcagctt cgccattttg caacaacgtc gcaatttcct gtggcgtccc
     2881 ctggattagc tcgagccgaa cctccgggaa aagttcgcga aaagctttaa tgacctctgg
     2941 caagctataa cgtgcctgag tatgcgtcgt tgcaatagtg agaacgccag acgtatcgtt
     3001 ggtaaacagg tctgcaagcc gacgaacatt actggcttca ttcagaatac gttctgcaat
     3061 gaccagtaat gctttgcccg gttcagtcat gcccagcagt cgcttacctc gtcgaacaaa
     3121 tatttcgatg ccaagttcat cctccagttc ccgaatatga cggctgacgc cggactgtga
     3181 ggtaaaaagc atattcgcaa cctctgtcag gttgtaatcc tgacgtgcag cctcgcggat
     3241 tatctttagt tgttggaaat tcacggtaaa ctccgggcag ttcagatttc ccgttattgt
     3301 taaagtctaa tgcccggcat aacaaataat aaaaacccgc atcttattcc atcccgatat
     3361 aacgcttagc tcaccaattg
//
LOCUS       DADMKF010000264         3325 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.269, whole genome shotgun sequence.
ACCESSION   DADMKF010000264 DADMKF010000000
VERSION     DADMKF010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3325
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.269"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(<1..321)
                     /gene="fliZ"
                     /locus_tag="JNC24_004048"
     CDS             complement(<1..321)
                     /gene="fliZ"
                     /locus_tag="JNC24_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HAY9160659.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDMLLDENGWQVEQKSWAALCRFCGDLHCKTQSDFFDIIGFKQFLFEQTEM
                     SPGTV"
     gene            complement(367..1086)
                     /locus_tag="JNC24_004049"
     CDS             complement(367..1086)
                     /locus_tag="JNC24_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HAY9160660.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDSNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLRTKLGKL"
     gene            complement(1251..2825)
                     /locus_tag="JNC24_004050"
     CDS             complement(1251..2825)
                     /locus_tag="JNC24_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin FliC"
                     /protein_id="HAY9160661.1"
                     /translation="MAQVINTNSLSLLTQNNLNKSQSSLSSAIERLSSGLRINSAKDD
                     AAGQAIANRFTANIKGLTQASRNANDGISVAQTTEGALSEINNNLQRIRELSVQATNG
                     TNSDSDLNSIQDEITQRLSEIDRVSNQTQFNGVKVLASDQTMKIQVGANDGETIEIAL
                     DKIDAKTLGLDNFSVALGKVPMSSAVALKSEAAPDLTKVNATDGSVGGAKAFGSNYKN
                     ADVETYFGTGNVQDTKDTTDATGTAGTKVYQVQVEGQTYFVGQDNNTNTNGFTLLKQN
                     STGYEKVQVGGKDVQLANFGGRVTAFVEDNGSATSVDLAAGKMGKALAYNDAPMSVYF
                     GGKNLDVHQVQDTQGNPVPNSFAAKTSDGTYIAVNVDAATGNTSVITDPNGKAVEWAV
                     KNDGSAQAIMREDDKVYTANITNKTATKGAELSASDLKALATTNPLSKLDEALAKVDK
                     LRSSLGAVQNRFDSAITNLGNTVNDLSSARSRIEDADYATEVSNMSRAQILQQAGTSV
                     LAQANQTTQNVLSLLR"
     gene            3080..3325
                     /locus_tag="JNC24_004051"
                     /pseudo
     CDS             3080..3325
                     /locus_tag="JNC24_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460913.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          852 a          792 c          712 g          969 t
ORIGIN      
        1 caccgtacct gggctcattt cagtttgctc aaaaagaaat tgcttaaagc cgataatatc
       61 gaaaaaatca ctctgcgttt tgcagtgtaa atcaccacaa aatcggcaca atgccgccca
      121 tgatttttgt tccacttgcc agccattttc atcaagcagc atgtccaggc gggaaatctc
      181 gattttattc actattttgc cgtcgcgaac gagggtaatg cggtcgagta atttacggca
      241 atgcgcgcaa tgagtctggc tgtgtttaaa gtctttaaga tagcggctta atggccgtct
      301 tttcaggtgc tgcaccatca ttaagaactc ctggtagtca aaattaaagt gcggcattta
      361 ctgacgttat aacttaccca gtttagtgcg taaccgttta atggcctggc tgtgtaactg
      421 actgacccgc gattccccga cctccagcac cgcgccaatc tctttgagat tcagctcttc
      481 ctggtaatag agggttaata ccagtttttc gcgctccggc aacgtttcga tggcttccat
      541 cacccgctgg cgcagattac tgtccagcag ttgttgtagc gggttttctc gctgatgatc
      601 atcagtaacc agttcgatgc tatcgccgtg ctcttcgcgc cactcatcgt aggagaagag
      661 ctggctgtta ttggtgtcga gcaacatttg gcgataatcg gcaatatcga tccctaaacg
      721 ttccgctacc tcagtttccg tggcgttgcg gccaagttcc tgctccagtt gccctattgc
      781 ctgtgccact tcacgcgcgt tgcgtcgcac gctgcgcggc acccagtcac ggctgcgaag
      841 ttcatccagc atagcgccac ggatacgctg cactgcgtaa gttgtaaatg ccgttccttg
      901 tagggcgtca tagcgttcga cggcattaag taacccaatg ccgcccgcct gtagcagatc
      961 gtcaagttcc acgctcgcgg gcagtcgaac ctgcaggcgc aatgcttcgt gacgcaccag
     1021 cgggacataa cgctgccaca gcgagtgttt atccattaca ccttcagcgg tatagagtga
     1081 attcacgata aacagccctg cgttatatga gttatcggca tgattatccg tttctgcagg
     1141 ttttttaatc ggacgattag tgggtgaaat gaggggttat ttaggggtta ccggtaaatt
     1201 gcggacagaa aaaaccccgc cggtggcggg gtttgagcga taagtgtaaa ttaacgcagc
     1261 agagacagta cgttctgcgt ggtctggtta gcctgtgcca gaacagaggt acccgcttgt
     1321 tgcaggatct gcgcacgaga catgttagac acttcggttg cgtagtcagc atcttctata
     1381 cggctacggg cagaagacag gtcgtttacg gtgttgccaa ggttggtgat ggcagagtcg
     1441 aaacggtttt gtactgcacc caaagaactg cgcaacttat caacttttgc caaagcttcg
     1501 tctaatttgg ataatggatt tgtggttgct aaggctttca aatctgaggc actcagttca
     1561 gcacctttgg ttgccgtctt attcgtgata ttggctgtat aaaccttatc atcttcacgc
     1621 ataattgcct gtgcagaacc atcatttttt actgcccatt caactgcctt accattagga
     1681 tcagtaataa cagacgtgtt acctgtagcg gcatctacat ttactgcaat gtaggtgccg
     1741 tctgatgttt tagcagcaaa tgaattaggt acaggattcc cttgggtatc ttgtacttgg
     1801 tggacatcta ggttttttcc cccaaaataa acagacattg gtgcatcatt ataagctaat
     1861 gctttaccca ttttacccgc agctaaatca actgatgtgg cagaaccatt atcttcaaca
     1921 aatgcagtta cacgaccacc aaagtttgct aactgaacat ccttaccacc cacctgaact
     1981 ttttcataac ctgtagagtt ttgtttcaat aatgtaaaac cgttcgtgtt ggtattatta
     2041 tcttgaccaa caaaataagt ctgcccttcc acctgtactt gataaacttt tgttcctgca
     2101 gtaccggtcg catcagttgt atcctttgta tcttgtacat taccggtacc aaaataagtt
     2161 tcaacatcag catttttata attgctaccg aatgctttag cacctcccac actaccatca
     2221 gttgcattta ccttagttaa gtcaggagcg gcttcgctct taagtgcaac cgcagaggac
     2281 attggaactt ttcctagtgc tacgctaaag ttatcaagcc ccaaggtttt agcatcaatt
     2341 ttatcaaggg caatctcaat ggtttcacca tcgttcgcac ctacttgaat tttcatagtc
     2401 tgatcagaag ccagaacttt tacaccatta aattgtgtct ggttagaaac acgatcaatt
     2461 tcactaaggc gttgtgtaat ttcatcctga attgaattca ggtcggagtc agagtttgta
     2521 ccattagtgg cctgtactga caattcacga atacgctgta agttattgtt gatttcagaa
     2581 agcgcacctt cagtggtctg cgcaacagaa ataccatcat tcgcgttacg ggaagcctga
     2641 gtcagacctt taatatttgc tgtaaaacgg ttagcaatcg cctgacctgc tgcgtcatct
     2701 ttagcactgt taatacgcag gccagaagag agacgttcaa tggcggagct cagagaagac
     2761 tgagatttgt tcaggttatt ctgggtcaac agcgacaggc tgtttgtatt aatgacttgt
     2821 gccatgatct tttccttatc aattacaact tgatgttatt gggctgttgc ccacggtttc
     2881 tcaccgtacc ccttgtatcg gcacctgaat ttcgaacttt agaaaatttt agtttctctc
     2941 ctgttttttc ttaacggctg aaatatccgt aatatacaac tctactctgc gcatcatctt
     3001 cgtgaatcaa ctattaaact ttgcgcacaa attgccgata acccgcttat ctactgtttt
     3061 caatcaaaag gaattaggta tggcttcatt tacatctctt ggcgttggtt cgaatctgcc
     3121 gctggataca ttgctaaaca acctgaccat cgctgaaaaa aaacgcttaa acccaattac
     3181 tcagcaacag agtgacaata ccgcacgtct gacagcctat ggcacattaa aaatgtgcat
     3241 tagaaaaatt ccagactgca aatactgcgt taaataaagc cgatctgttc aaaagcacta
     3301 ccgttaccag tagcaatgaa gacct
//
LOCUS       DADMKF010000265         3308 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.270, whole genome shotgun sequence.
ACCESSION   DADMKF010000265 DADMKF010000000
VERSION     DADMKF010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3308
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.270"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(<1..843)
                     /gene="adrA"
                     /locus_tag="JNC24_004052"
                     /gene_synonym="dgcC"
     CDS             complement(<1..843)
                     /gene="adrA"
                     /locus_tag="JNC24_004052"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HAY9160662.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMIMCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVVYPLLFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRG"
     gene            complement(945..1265)
                     /gene="psiF"
                     /locus_tag="JNC24_004053"
     CDS             complement(945..1265)
                     /gene="psiF"
                     /locus_tag="JNC24_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HAY9160663.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQSLKGDDRNKFMSACL
                     KKAA"
     gene            complement(1384..2799)
                     /gene="phoA"
                     /locus_tag="JNC24_004054"
     CDS             complement(1384..2799)
                     /gene="phoA"
                     /locus_tag="JNC24_004054"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HAY9160664.1"
                     /translation="MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTSDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVNDAASLNSVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKTIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKEGNTLVIVTADH
                     AHASQVVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(2900..3160)
                     /gene="iraP"
                     /locus_tag="JNC24_004055"
     CDS             complement(2900..3160)
                     /gene="iraP"
                     /locus_tag="JNC24_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HAY9160665.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPRQ"
BASE COUNT          782 a          874 c          783 g          869 t
ORIGIN      
        1 accgcgcagg gtaatttgta actgtcgggt aagcgccaca atcgcttcat cgcccacatc
       61 atgaccccag gtatcgttga tgctcttgaa atggtcgata tcgataatca gtaacgttgc
      121 atcgcgatta tgccgccgac agttatcaaa ttcattgcgt aacatagttt cccaatgacg
      181 tcggttatac acgcccgtca tgccgtcgcg agtactcatg acctgcaacc tgcgtttatg
      241 ttccgccagt ttggttgccg tctggtagct gacccagcca aacagcagag gataaacgac
      301 aataatggga agggagagcc accattccag cggcgcacta ttgaacgaca cggtaatgcc
      361 cgtaagctcg agggtgacaa ggcaggaaac caccatcaac accagacccg cgacaaacag
      421 acgggggccg cctgccccca tcaaattcag acacataatc atcaacatag cggtggaagg
      481 cagcacgttt acgcccatta cgcctaccca cattcccgct aatactgcat cggtttttaa
      541 gttgtaaatt tcccggctaa gcggatcgac ggccctgctc gctatctgcc aggctaaatg
      601 cggccagacg aacgcccagc cgaccaacac cagccaccac cagcccggcg gcgggtgcga
      661 aaccagcgtt gaagcaatcg gtaagaacat gccagccagg ccaaccgcac ggggtagtct
      721 gacgcgacgg gcgaagcgca gcccggaccg ctgatgttcg ttttgaggag ttaaaggagg
      781 ttcctcccca tgcgccgccg cttttttgaa aaagttttca tcattcatta tttttgggaa
      841 cattcagaaa caattttccc aaattataga gacggggcta aaggcagaag tatgaaattt
      901 cgggtgagcc actttcgtag ctcacccgtt agcactatca ggtatcaggc ggctttcttg
      961 aggcaggcac tcataaactt attacgatca tcacctttca gagattgttg cgttgcttga
     1021 ttattacatt cgcgcatctt ttgctgctgt ggcgtcaaac ttttttcacc aggcgcagac
     1081 ttgctgttct tcaggcaatc actcatgtag gtcttacgag catccccttt caacgcctgc
     1141 gccgtcgcct gctgattaca ggaggtcata cgctgttgtt gtggggttaa ggttctctcg
     1201 gcagcgccga cggtggttaa aaaaaccaga ccaaaaagca aggtaaccag taatgttatt
     1261 ttcatagcac catccctctt catgttttaa ccatgagcgt ttgcgcccgt gatctgccat
     1321 taagtctggt tgctaacagc aaaaaaacca cccggcagcg aaaattcact gccgggcgcg
     1381 gttttatttc agccccagag cggctttcat ggtgtagaag agatcggtct ggtcggtcag
     1441 tccaacaaca ttggcggcat gcggaccata cgccgcaata cgcaactgac tgccggtatg
     1501 ttcttgtgaa tcctcttcgg aattcccgta actcatcacc atcactgcgc catctttagt
     1561 atttagcgcc tgagtgaggc ccggcgcttt ggtatccggc gcaacaacct ggctggcgtg
     1621 ggcgtgatca gcggtgacta tgaccagcgt gttaccctcc tttttagcga attccagcgc
     1681 ccgttgtacg gcttcatcga gatcgaccgt ctcgccaatt tgcccacaag gattcgcagc
     1741 gtgatcctgt ttatcgattg acgcaccttc aacttgcagg aaaaatcctt tctcattttt
     1801 actcaacaat tcaatggttt tgtcggtcat ctgtgccagg gttggtacac tgtcattacg
     1861 ttgcggatta ggcgtacagg tgactgcggg cttatcgata ttgccgtggt acgttgcttt
     1921 cggccctagc cagcgcactg gcatattgcc gtcagcaaac aggccaagca ggggtttttg
     1981 ctgattcgct tccgtcaccg aattcagtga ggcagcatcg ttcaccaact gataaccacg
     2041 cgcctgtgcc tgttcacgca gcgtttttcc ctgccattca ccagcggttg ccgtttcagc
     2101 aaaggttttt gcgccgccgc caagcgtaac gtcggcacga gcgttaagca gctgttcggt
     2161 aatcgatcct tttccgcctt tttccagagc gttacccgga catttttcac tggtcgcgct
     2221 cggaccgtag catttgcgcg aagtcacatg tgccaccagc gcagcgggcg tggcatcctg
     2281 caactctgcg gtagaaacgt taccggtcgc cagacctgcg gcttttgcca tttccagaat
     2341 cgttgggtga tctttttcgt gaatatcgac gcccagcgcg ccgttatagg ttttgacacc
     2401 ggttgaccag gcggttgctg atgcagccga gtcggtgacg tagtccggtt tgccagtttt
     2461 tttattcagc gcatagtgag tgtattgccc ggtaagcggt aaggcatcga tacctttaaa
     2521 aaagccgccc gcaccttcgg cataattacg tgcggcagta atttccgaat cccccatccc
     2581 atcgccaatc agcaaaataa tattttttgc aggtttatcg ctaagagaat cacgcagagc
     2641 ggcggtctga tcactcgtta agcggcgagc accgccgggt gcagtaatat cgccctgagc
     2701 agcccggttt tccagaacag gcatttctgg tgtccgggct tttgtcacag gggtaaacag
     2761 taacggtaag agtgccagtg caatagtgct ttgtttcact ttattttctc catgtacaaa
     2821 tacattaaaa aataaaaaca aagcgactat aagtctcggc cgtgacaact ttatgacagc
     2881 tgttgaaaag attaactttt tactgacgag gatgcttcaa taacttcttt acgtaatcgc
     2941 gcagcagctc cgtatcgtcg tcaggaatgc tggcatcggg ctttacctcg tacagcgccc
     3001 cctctacctg atcaatcaac cgctgttggt cattttgcgc catattgcga agcattgcag
     3061 tgacgataat ctccaaagct tctacctgcg cacacagttc tttcgactct tcttcttttt
     3121 gggcaagctt aaataacaac tcagcaatga gatttttcat gtctgtattt ccttatccaa
     3181 agtatggaga agttaacatt atgtttcaca acttcatagt gtctggaagt attattttta
     3241 tgacaaagga aatactttgc gcaaatattt tttgtttgat taccagcgaa acgtttctct
     3301 tatttaaa
//
LOCUS       DADMKF010000266         3285 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.271, whole genome shotgun sequence.
ACCESSION   DADMKF010000266 DADMKF010000000
VERSION     DADMKF010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3285
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.271"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(<1..229)
                     /locus_tag="JNC24_004056"
     CDS             complement(<1..229)
                     /locus_tag="JNC24_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9160666.1"
                     /translation="MQSITPPLIAVIGSDGSGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRAVTKLPLMGKSLHKTIERNQVKTAKK"
     gene            403..1116
                     /locus_tag="JNC24_004057"
     CDS             403..1116
                     /locus_tag="JNC24_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HAY9160667.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSPLTASLVNELAARMPT
                     EHIYLGQSSGLIGEWISQLPVIGAPFGRYLRSKAAHVHEKPSTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGFLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGARILELDGR
                     QPADEILQASLRAIHAALS"
     gene            1148..1906
                     /locus_tag="JNC24_004058"
     CDS             1148..1906
                     /locus_tag="JNC24_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9160668.1"
                     /translation="MDALQTQTVNSTTAPQPSYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCLSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERRLYQQMAQYRPELIIRLGIDIETAISRKPDHDYAELQDKIGVMSKIGYNGTK
                     ILEIDSRAPYSEVLEQAQKAVSLVAIVSDRRSLT"
     gene            1952..>3285
                     /locus_tag="JNC24_004059"
     CDS             1952..>3285
                     /locus_tag="JNC24_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HAY9160669.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVVSALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVVAWYFDFGFAGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVLI
                     AGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVVLSLIPTLKAFFQRHSLLYNAA"
BASE COUNT          750 a          790 c          854 g          891 t
ORIGIN      
        1 atttttttgc tgttttcacc tgatttcgtt caattgtttt atgtaaggat tttcccatca
       61 acggtaattt tgtcactgca cgaccgacat ttccggcctg ttttcctaaa tgaactcttt
      121 cggcagcacc atatttttcg acaacggtaa taagatgttc acacaccgtt gacttgcctg
      181 aaccatcgct accaataacg gcaattaatg gaggtgttat tgactgcatg aatatatcct
      241 ttaatttaaa catccattaa aaatatttat ttggttaata tgtttttatg aaagcgtaat
      301 tcaggtcaat gtcacaatta accattgtca caataaggtt gaacggatat tctgagcacg
      361 gaccacgtaa tattcaatac attatttact gccgggtttt tcgtgaatca acgtaatatg
      421 tcaataatta attccacgcc agtgcgtgtt attgccattg taggatgtga tggttcaggt
      481 aaatcgcccc tcacggcaag cctggtaaat gaactggcag caagaatgcc aacagaacac
      541 atttatctcg ggcaatcgtc cgggctaatt ggcgaatgga tttcacagct ccctgttatt
      601 ggcgcacctt ttgggcgtta tctgcgaagt aaagcggcac atgtgcacga aaaaccctca
      661 acaccgcctg gcaatattac tgctctggtt atctatctgc tttcctgctg gcgggcgtac
      721 aagtttcgca aaatgttgtg taaaagccag caaggctttc tgctcatcac cgaccgctac
      781 ccgcaggttg aagtgccggg ttttcgcttt gatggcccgc aattggcaaa aaccacgggc
      841 ggtaacggtt ggataaaaat gttaaggcag cgcgagctga agctgtacca atggatggca
      901 tcttatttgc ccgtattgtt gattcgtctt ggcattgatg aacaaaccgc gtttgcgcgt
      961 aaacctgacc accaactggc agcgttacag gaaaaaatcg ccgttacgcc gcaactgact
     1021 ttcaacggcg ccaggatcct tgaactggat ggtcggcaac cagccgatga aatcctgcaa
     1081 gcgtcactac gtgcaattca cgccgcgctt tcctgatcac caaaatggat aggggatgat
     1141 ttaacgaatg gatgcactac aaacacaaac tgttaatagt acaaccgcgc cgcagcccag
     1201 ctacattccg gggctaattg cggtggtcgg gtgcgatggc accggtaaat ccacgctgac
     1261 caccgatctg gtgaaatcat tgcaacagca ctggcaaacc gagcggcgct atctggggct
     1321 gctctcgggc gaagacggcg acaaaatcaa acgattaccg ttggttggcg tctggctgga
     1381 acggcgactg gcggccaaat cctcgaaaac ccaaagcatg aaaaccaaat ctccggcgct
     1441 atgggcggcg gtgattatgt actgcttatc gctgcgaaga atggcgaatc tacgcaaggt
     1501 tcagcgactg gcgcaaagtg gcgttctggt ggtcagcgat cgcttcccgc aggctgaaat
     1561 ttcgggcttt tattacgacg gaccggggat tggcgtcgaa cgtgcgaccg ggaaaatcag
     1621 catgtttctg gcgcagcgcg aacggcgttt ataccaacaa atggcgcaat atcgcccgga
     1681 attaattatt cgcctgggca ttgatattga gactgccatc tcccgcaagc ctgaccatga
     1741 ctatgccgag ctgcaggaca aaatcggtgt catgtcgaag attggctata acggcacaaa
     1801 aattcttgag attgattcca gagcgcccta cagcgaagtg ctggaacagg cacagaaggc
     1861 ggtttctctg gttgccatcg tttctgaccg ccgaagttta acttaaggct ggcgggatac
     1921 tttttactat caacgataag gtcaggctga attggcgggt tttaacatca aacattggtt
     1981 tgcagatggc gcgtttcgca ccattattcg caatagcgcc tggttaggct ccagtaatgt
     2041 cgtgagcgcc ttgctggggc tgttggcgct ctcgtgtgcc ggtaaaggga tgacgcccgc
     2101 catgtttggc gtactggtga ttgtgcaatc gtacgccaag tcgatcagcg attttattaa
     2161 gtttcagaca tggcagctgg tggttcagta cggaacgcca gccttgacca acaataatcc
     2221 gcagcaattt cgcaatgtcg tctcattttc cttctcgctg gatatcgtca gcggcgcggt
     2281 ggcgattgtc ggcggcattg ccttactgcc attcctttcc cattcattag gtctggatga
     2341 ccaaagcttt tggctggcgg cgctgtattg cacgctcatt ccttcaatgg cttcctccac
     2401 gccgaccggc attctgcgtg cggtagatcg cttcgattta attgctgtac agcaggcgac
     2461 gaaacctttt ctgcgcgcag cggggagcgt cgtagcctgg tattttgact ttggttttgc
     2521 gggttttgtt attgcctggt acgtgtcgaa tctggttggc ggcaccatgt actggtggtt
     2581 tgcagcgcgc gaattacgcc gcagaaatat ccataatgcc ttcaaattga atctgtttga
     2641 gtctgcccga cacatcaaag gcgcgtggag ttttgtctgg tcaaccaaca ttgcccactc
     2701 catctggtcg gcgcgtaact cgtgcagcac agtgctggtg gggatcgtgt taggacccgc
     2761 tgccgccggg ttatttaaaa tcgccatgac attctttgac gccgccggaa cgccagcggg
     2821 tttgctgggt aaaagttttt acccagaggt gatgcgttta gatccgcgca ccaccagacc
     2881 gtggttgtta ggtgtgaagt ccggtttact ggcgggcgga atcggtattc tggtggcgct
     2941 cgcggtgttg atcgccggca agccgctcat ttcgctggtg tttggcgtta agtatctcga
     3001 agcgtatgac ctgattcagg tgatgctggg cgcaattgtg atttcgatgc tgggtttccc
     3061 gcaggagtca ttgctattga tggcgggaaa acagcgcgct tttctcgtgg cgcaaaccat
     3121 cgcctcaatc ggctacatcg tattgctgtt catgttctgt catctgtttg gtgtgctggg
     3181 agcagcgttt gcttacttcg gtgggcaatg tctggatgtg gtgctctcgt taattccaac
     3241 tttaaaggca ttttttcagc gtcattcctt gctttataac gcagc
//
LOCUS       DADMKF010000267         3222 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.272, whole genome shotgun sequence.
ACCESSION   DADMKF010000267 DADMKF010000000
VERSION     DADMKF010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3222
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.272"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            8..511
                     /locus_tag="JNC24_004060"
     CDS             8..511
                     /locus_tag="JNC24_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAY9160670.1"
                     /translation="MNNIAPQSPVMRRLTLLDNPAIARVIRQVSAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYEGEVVGGGGIAPLTGSESDICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIALYEHLGFEHIDYALGCTGHVDCE
                     VRMLREL"
     gene            complement(557..973)
                     /gene="rraB"
                     /locus_tag="JNC24_004061"
     CDS             complement(557..973)
                     /gene="rraB"
                     /locus_tag="JNC24_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HAY9160671.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            1135..2139
                     /gene="argF"
                     /locus_tag="JNC24_004062"
     CDS             1135..2139
                     /gene="argF"
                     /locus_tag="JNC24_004062"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HAY9160672.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKTFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     "
     gene            complement(2211..2873)
                     /locus_tag="JNC24_004063"
     CDS             complement(2211..2873)
                     /locus_tag="JNC24_004063"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9160673.1"
                     /translation="MQYFGDSEKIKYACSVINHIDFSCDQSKDFSCDQSKIKIAETLF
                     FNLDKEPYKNSRKLQELIWDKLVAYVNDFSLSNQEKSRLILRLFDDVKLLFNEVPVSI
                     LVNDIFLKDFFMKQPDFAKWYFYQLLKCYEGEQLYLNELGYVYGDEEKTKEIVNKLPG
                     YVVKIFEEKMGNELKIRTRMMKILRNGKINIYGYINEEQLGKLNPPEDLRIAIKKLGW
                     NN"
     gene            complement(2881..>3222)
                     /locus_tag="JNC24_004064"
     CDS             complement(2881..>3222)
                     /locus_tag="JNC24_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001379308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAY9160674.1"
                     /translation="DLLEKPLNNALLAIRKEHLLLMPYVCNESISYLLGEKGILKEID
                     DLNAVNNYLLNNKKATDNEINDIKVNLSHILIDSLDDAKVNLTPVIDSILETFLKSPY
                     INDVRIPGLVF"
BASE COUNT          893 a          720 c          676 g          933 t
ORIGIN      
        1 ttaaactatg aataacattg cgccgcaatc acctgtaatg cgtcgcctga cgctgctgga
       61 taatcctgct atcgcccgcg tcattcgtca ggtatccgcc gaatacggtc ttaccgctga
      121 taaaggctac accgtcgccg atccgaatct tgacgagctg tatcaagtgt atagtcagcc
      181 tggccatgca tattgggtgg ttgagtacga gggtgaagtg gtcggcggcg gtgggattgc
      241 gccattaacc gggagtgagt cggacatttg cgaactgcaa aagatgtatt ttcttccggc
      301 tattcgcggt aaagggctag cgaaaaaact ggccttaatg gcgatggagc aggcacgaga
      361 gatgggcttc aaacgctgct atctggaaac gaccgctttt ttaaaggaag ccattgcgct
      421 ttatgagcat ttgggctttg agcatatcga ctatgcgctt ggctgcacgg gccatgtcga
      481 ttgtgaagtg cggatgctac gcgaactcta aaaaaatgcc cgctagtatg tgctatcggg
      541 cattttaatc aattaattag tggcgaaccc cgtcatcgtc ttcatcgaca aaatcttcat
      601 cgtcgccatc ttcgccgtta ggatcttcaa agtaagtgcc ccaaccgtcg tactcaacgt
      661 caaatttctc cgccagcgtc atcagttgtt caacctgggc atcgatcaga tcggcattca
      721 acgcgcactc gctgaggatg tcgcagcaaa tcacgatatc accatcttca acttccagct
      781 cttctggatc ggtcacttcg taaccgagtt taaacgcttc aactgccgct ttttccagag
      841 tttccagatc gtctgcggaa agatggtgtt cgatggtgta caacgcgtcc ggatcgctgc
      901 catcttccag taattcttca ataatcaaac gtgtttcttc acgctgttct tccagttgtt
      961 ccgggtttgc catggctcat tcctcttaat gtcctgccga tactcttatt gtcacacacc
     1021 actgacattg cctccacctt tgtgataaag atttatgctt tagacttgca aatgaataat
     1081 catccatata aattgaattt taattcatta aggcgttagc cacaggaggg acctatgtcc
     1141 gggttttatc ataagcattt cctgaaatta ctcgatttca cgccagctga actcaacagc
     1201 ctgctgcagt tagccgcgaa gctgaaagcc gataagaaaa gcggtaaaga agaagccaaa
     1261 ctcactggta aaaacatcgc gctcatcttc gaaaaagact cgacccgtac ccgatgctct
     1321 ttcgaagttg ccgcatatga ccagggggct cgtgttactt atctcggccc aagcggcagc
     1381 caaattggtc ataaagagtc gattaaagac actgcccgcg tgcttggtcg catgtatgac
     1441 ggtattcagt atcgcggcta tggtcaggag attgtcgaaa cactggcgga atacgctggc
     1501 gtgccggtat ggaacggcct gaccaatgag tttcacccca cgcagctgct ggcggatctc
     1561 ctcaccatgc aggagcattt gcccggcaaa acgttcaacg aaatgacgct agtctatgca
     1621 ggtgacgcac gtaacaacat gggcaattcg atgctcgaag ctgcagcgct taccggtctg
     1681 gatttgcgtc tggtcgcgcc acaggcatgc tggccggaag ctgcgctggt tacggaatgc
     1741 cgcgccctgg cacaacaaaa tggcgggaat attacgctga cggaagatgt agctaaagga
     1801 gttgaaggtg ctgactttat ctataccgat gtatgggtgt cgatgggtga agcaaaagag
     1861 aaatgggctg aacgcattgc attgctgcgt gattatcaag tgaacagcaa gatgatgcag
     1921 ttgaccggta acccggaggt caaattcctc cactgcctgc ccgcgtttca tgacgaccaa
     1981 acgacgcttg gcaaaaaaat ggcggaagag tttggcctac atggcggaat ggaagtgact
     2041 gatgaggtct tcgaatctgc cgccagcatt gtatttgatc aggcggaaaa ccgcatgcat
     2101 accatcaaag cggtgatggt cgcgacgctc agtaaataat tgatatcgct ataaagatgc
     2161 cgggaaatac aaaaacatcc cggcgtcaca ttattttatg cagtgatagt tcaattattc
     2221 caccccaatt tttttattgc tatacgtaaa tcttccggag gatttaattt tcctaactgt
     2281 tcttcattaa tatatccata tatattgatt ttgccattcc ggaggatttt catcattctt
     2341 gttctaattt ttaattcatt acccatcttt tcttcaaaaa tcttaacgac ataacccgga
     2401 agtttattta caatttcttt tgttttttct tcatcaccat aaacatatcc taactcatta
     2461 agatacagct gttcgccctc ataacactta agtaattgat agaagtacca cttagcaaaa
     2521 tcaggttgct tcatgaaaaa atccttcaaa aaaatatcat ttactaaaat gctgacaggc
     2581 acttcattaa atagtaactt aacatcatca aataacctta gtattaagcg tgatttttct
     2641 tgattgctta agctaaaatc attgacatat gcaaccaact tatcccaaat caattcctgt
     2701 aatttacggc tatttttata gggttcttta tcgagattga aaaataaggt ctcagctatt
     2761 ttaattttag attgatcgca gctaaaatct ttagattgat cgcagctaaa atcaatatga
     2821 tttatcacgg agcatgcata ctttatcttt tcagaatcac caaaatattg cattctttta
     2881 ttaaaacacc aatccaggta ttcttacatc attaatatat ggggatttta aaaaagtctc
     2941 cagaatcgaa tcgatgaccg gtgtaaggtt aacttttgca tcatctaagg aatcaataag
     3001 aatatgagac aggttaactt tgatgtcatt aatttcattg tcagtagctt tcttattatt
     3061 aagaagataa ttatttactg cgttaaggtc atctatttct ttcagtatcc ctttttcacc
     3121 aagcaagtat gaaatcgatt cattacatac ataaggcatt aatagcagat gttctttcct
     3181 tattgctaat aatgcattgt tcaatggttt ttctaataaa tc
//
LOCUS       DADMKF010000268         3209 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.273, whole genome shotgun sequence.
ACCESSION   DADMKF010000268 DADMKF010000000
VERSION     DADMKF010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3209
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.273"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(140..1352)
                     /locus_tag="JNC24_004065"
                     /pseudo
     CDS             complement(140..1352)
                     /locus_tag="JNC24_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709437.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(1624..1718)
                     /locus_tag="JNC24_004066"
     tRNA            complement(1624..1718)
                     /locus_tag="JNC24_004066"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1682..1684),aa:Sec,seq:tca)
     gene            2011..>3209
                     /locus_tag="JNC24_004067"
     CDS             2011..>3209
                     /locus_tag="JNC24_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
                     /protein_id="HAY9160675.1"
                     /translation="MKSEVLSVKEKIGYGMGDAASHIIFDNVMLYMMFFYTDIFGIPA
                     GFVGTMFLVARALDAISDPCMGLLADRTRSRWGKFRPWVLFGALPFGIVCVLAYSTPD
                     LSMNGKMIYAAITYTLLTLLYTVVNIPYCALGGVITNDPTQRISLQSWRFVLATAGGM
                     LSTVLMMPLVNLIGGDNKPLGFQGGIAVLSVVAFMMLAFCFFTTKERVEAPPTTTSMR
                     EDLRDIWQNDQWRIVGLLTIFNILAVCVRGGAMMYYVTWILGTPEVFVAFLTTYCVGN
                     LIGSALAKPLTDWKCKVTIFWWTNALLAVISLAMFFVPMQASITMFVFIFVIGVLHQL
                     VTPIQWVMMSDTVDYGEWCNGKRLTGISFAGTLFVLKLGLAFGGALIGWMLAYGGYDA
                     AEKAQNSA"
BASE COUNT          804 a          719 c          750 g          936 t
ORIGIN      
        1 tcaatttgtt tttaattatg tttttgtaat aagtttgaag acgcaaaaaa aataatataa
       61 taacaaaaat cctccagcct tgtaggccag aggataaaat acttaattga ggaaaatatc
      121 agggaaaagt atttctgact taactttccc ggctggagag tgaatcaatg taatcagcgt
      181 accattgcat catttcacgg cggccatcga gatacagggc atgattatag gtacccctga
      241 tggaattttt atccacatga gctaactgca tttcaatcca ggcggaatta aagccttgtt
      301 catgcagaat tgtactcatg gtgtgtctga aaccgtgccc tgtgagtctt cccttatatc
      361 caagtaattc aattacttgg ttaatgcttt ccttactaat tggtttgcgg ggatcatttc
      421 taccgataaa gactagggga tgatgttttg agattggttc aagctgctta aatagcataa
      481 tgacctgctc agagagcggc acaatatgag gtcgcttcat cttcattact tctgccggga
      541 tctcccacaa ttttgtctca aaatcgatat cttcccagcg tgcaaaacgc agttcttgtg
      601 ttcgtacgcc ggttaacata atgatctgag tagctgtctt agtgattata cttccggtat
      661 aacccgccag atcgttgagg aagtggggaa gttcattagc agtaagaaag ggaaaatgta
      721 cttttttcgg tgtagctaaa gcactggcaa gatcgggggc tgggttatag tctgcccggc
      781 cagtaacaat tgcatagcgg aacacctcac cacagcgctg gcggaccttg cgcattttct
      841 ccagagctcc acgtttttcc attttacgca gtgcttccag taactccatt ggtttgattt
      901 cggcaatagg gcgcttgcca atgtagggaa aaatgtcttt ttcaaaggta tcaatgattt
      961 catcacgata gcgtaatgac caccgatcag cttttgactg gtgccattcg cgagcaatag
     1021 cctcgaacgt atttttcaga ctcatttgct gcgcgatttt ttcttccttt tttgcctccc
     1081 ctggatcgcc tccagcagcg aggagtttcc tggcttcact gcgtttctcg cgagcatcgg
     1141 ctagcgttac atcggggtat acgccaatag aaagcatctt ttctttacca gcaaagcggt
     1201 acttcatgcg ccagtattta gaaccattag tgttaatcag tagatacata ccgccgccat
     1261 cagcaagctt atagggtttg tctttgggtt ttgcggtttt cactttgata tcggttaatg
     1321 ccatgacatc ctccggcttt tgatgaattc atgttcctga gggggatttc tgccagtaac
     1381 taacctgata cccccaatac accccttgta taacaaagat attgttaaaa gccgttgtta
     1441 tgtatcacat ttttcctatt atgggggtgc ttgtatttca ataggaaata acacccccta
     1501 aagtaccccc aaaagaaact tgatatgggg tgaagtaggt tgacctcagg atacaaataa
     1561 atactgatat acaagggatt agaagagatt tattgacatt gggagagttc agaagaggta
     1621 aactggcgga agatcacagg agtcgaacct gcccgggacc gctggcggcc ccaactggat
     1681 ttgaagtcca gccgcctcac cggagacgac gatcttccgc gcctcgattg ctacatggag
     1741 gcggggcgca ttatagctac ttccttgagt ttctacatcc cccagatcag ttttttccgc
     1801 ctaatacacg cagcgtatta tcacgaaagc attataactc atataaaacc catattttac
     1861 aatgatatat gagcggaatt aacgctacca cgatcacata aaacaagaat cataaattaa
     1921 taaccagata tcggaatatt cgctctcaca gggatggttt acaaaatgcg ttatcggtct
     1981 cagcacccct atagcatcaa ggaaaagcag atgaagagtg aagtgttgtc cgttaaagag
     2041 aaaattggtt atggcatggg agacgccgcc agccacatta ttttcgataa cgtaatgtta
     2101 tatatgatgt tcttttatac cgatattttt ggcattcctg ccggatttgt cggaaccatg
     2161 tttttggtcg ctcgtgcact ggatgcgatt tccgatcctt gcatggggtt gttggccgat
     2221 cgaacgcgct ctcgctgggg taaatttcgt ccgtgggtac tgtttggcgc actgccattc
     2281 gggatcgtct gtgtactggc ctatagcacg ccagatctca gtatgaacgg caaaatgatc
     2341 tatgcagcaa ttacttacac cctacttacc ttactttata ccgtcgtcaa tatcccttac
     2401 tgcgcattgg gtggtgtaat caccaatgac ccgactcagc gtatctcgct gcaatcctgg
     2461 cgttttgtgc tggcgaccgc gggaggcatg ctttctactg ttctgatgat gccactggtt
     2521 aatttaattg gcggtgataa taaaccactc ggtttccagg gcggtatcgc ggtcctttcc
     2581 gtggtggcat tcatgatgct ggcattttgt ttcttcacca ctaaagaacg cgttgaagca
     2641 ccacctacaa caacgtctat gcgggaagat ttacgtgata tctggcaaaa cgaccagtgg
     2701 cggattgtcg gtttactaac cattttcaat atcctggcgg tgtgcgtacg cggtggggcg
     2761 atgatgtatt acgtcacatg gattttgggc acgccggaag tgtttgtcgc ttttctcacc
     2821 acttattgcg tgggtaacct gattggttcc gcactggcaa aacctctgac cgactggaaa
     2881 tgtaaagtca ctatcttctg gtggacgaac gccctgctgg cagtgattag cctcgcgatg
     2941 ttctttgttc ccatgcaggc cagcatcact atgtttgtct tcatcttcgt gattggtgtg
     3001 ttgcatcaac tggtgacacc tatccagtgg gtaatgatgt ccgataccgt cgactacggc
     3061 gagtggtgca atggtaaacg cctgaccggg atcagttttg ctggcacgct gtttgtgctc
     3121 aaactggggt tggccttcgg cggcgctctt atcggctgga tgctggctta tggcggatat
     3181 gatgcggcag aaaaagcgca gaacagcgc
//
LOCUS       DADMKF010000269         3206 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.274, whole genome shotgun sequence.
ACCESSION   DADMKF010000269 DADMKF010000000
VERSION     DADMKF010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3206
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.274"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(60..587)
                     /gene="mobB"
                     /locus_tag="JNC24_004068"
     CDS             complement(60..587)
                     /gene="mobB"
                     /locus_tag="JNC24_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418293.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HAY9160676.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTEKPDEEELDLQFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
     gene            complement(569..1153)
                     /gene="mobA"
                     /locus_tag="JNC24_004069"
     CDS             complement(569..1153)
                     /gene="mobA"
                     /locus_tag="JNC24_004069"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HAY9160677.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLELNGKPLWQHVADALM
                     TQLSHVVVNANRHQEIYQASGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPHVLASRLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
     gene            1223..1492
                     /locus_tag="JNC24_004070"
     CDS             1223..1492
                     /locus_tag="JNC24_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HAY9160678.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDLNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            1569..2555
                     /gene="srkA"
                     /locus_tag="JNC24_004071"
     CDS             1569..2555
                     /gene="srkA"
                     /locus_tag="JNC24_004071"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HAY9160679.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQF
                     QDEERRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQTEAVGRYLGRMHQTGHKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAEQRMQLETIIEAYEEFGEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRRWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            2572..3198
                     /gene="dsbA"
                     /locus_tag="JNC24_004072"
     CDS             2572..3198
                     /gene="dsbA"
                     /locus_tag="JNC24_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HAY9160680.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEKK"
BASE COUNT          820 a          773 c          778 g          835 t
ORIGIN      
        1 ttggcgcaga aaaaaatgcc ggatgcgaca ctggcgcgtc ttatccagcc tactcttgct
       61 tatccgttct gcttttgcat ccactccacc acaaaatcag ccagcccctc aacatcatta
      121 atatccagta atgcgacatc aagattaagc ggcacatcac tggctacagc aataacatgc
      181 ctgtctatca ctaattcttc aggtcgatgt ccggctccat cgcgaaacag cacaatcttt
      241 gcgatctctt catgcttaaa cccttcgacc agaatcaaat ccagctttga ggtatccatt
      301 cgacttgcga gaaactgtag atccagctct tcttcgtctg gtttttccgt catcaaggcc
      361 catcgttgct ggctggcaac gatggtttgt gccgcgccag ccttgcgcag ctcatagcta
      421 tctttgcctg gcttatcaac atccatatca tggtgcgtat gcttaatcag ccctggacgg
      481 atccctctgg cgcataatgc cgggatcagt tttttcaaaa gcgtagtttt tccggtacca
      541 ctccacgcgg caaaggcgag taacggtatc atcgtttttc ctgccatcgg gcaagctcct
      601 ctggcgtatt cacgttaaca aatgcatctt tatgatcgct gaaatcaacc gcatggccgc
      661 cagccagacg cataaatgcc attacccggc gttctcctgc ttgcagatat tctagtaata
      721 aaggctcaat agcacggttt accagagcaa tagtcgggtg atcgcgttca ccgtcatgga
      781 cccacacgac aggcgcatct ttgcgctgat gattaagccg ggaagctaaa acatggggaa
      841 tgtaaggcgt atcgcacgga caaaacaaaa accactcacc agcttcctgc tgcattactg
      901 aaagcattcc tgccagaggg cctgggtaat ccgccagtga atcttcaatc actttcagac
      961 cgcttgcctg atagatttcc tgatgacgat tggcattaac cacgacgtga gagagctgcg
     1021 tcataagcgc gtcagcgaca tgttgccata atggtttgcc gtttaattca agcaatcctt
     1081 tatctacgcc gcccattcgt ctggctttac cgcctgccag cacaacgcct gttatcgtcg
     1141 tcatcagatt caccgatatc gcctctttta ttgtgggatt gaccctgcta acgtgtctgt
     1201 ctcaagagta aggagcatca ctatgaaatg taaacgtctg aatgaagtta ttgaactcct
     1261 ccagccagcc tggcaaaaag agccagacct taacctgttg caatttttgc agaaactggc
     1321 gaaagagtca ggttttgacg gcgaactggc ggatttgacg gatgacattc tgatctatca
     1381 cctgaaaatg cgtgattccg cgaaagatgc ggtgatcccg ggtttgcaga aagattatga
     1441 agaagacttc aaaacggcgc tgttacgcgc tcgcggcgta attaaagagt aaaagcttgt
     1501 aagcggcgcc accaaaatca tcgtgaaatg atatccttca tcattcgtaa tgttttccgg
     1561 atgatgggat gaacaacagc gcttttactt tccagacact acacccggat accatcatgg
     1621 acgctctgtt tgagcaaggg atccgggtgg attccggtct taccccgctt aacagctatg
     1681 aaaaccgtgt ctatcaattt caggacgaag agcgtcgacg ttttgtcgtc aaattttatc
     1741 gccctgaacg ttggacagcc gatcaaatcc tcgaagaaca tcaatttgcg ttgcagctgg
     1801 taaatgatga agttccggtc gcagcacctg tggcctttaa cggtcagact ttattgaatc
     1861 atcagggatt ttatttcgct gtttttccaa gcgtcggtgg tcgccagttc gaagctgata
     1921 atatcgatca gacggaagcg gttgggcgtt atttagggcg tatgcaccag acggggcaca
     1981 aacagctttt tatccatcgc ccgaccatcg gtctgaatga atatctcatt gagccacgca
     2041 agctgtttga ggacgctaca ctgatacctt ccgggttgaa agcggcattc ctgaaagcga
     2101 cagatgagct gatcgccgcc gttacagcac actggcggga agatttcacc gttctgcggc
     2161 tacatggaga ctgccacgcc gggaatattc tctggcgcga tggtccaatg tttgttgatc
     2221 tggatgatgc acgtaatggt ccagctattc aggatttgtg gatgttgctc aatggcgata
     2281 aagccgagca gcggatgcaa ctggaaacta ttattgaagc ttatgaagaa tttggcgagt
     2341 tcgacaccgc tgaaatcgga ctgattgaac ctttacgcgc catgcgtttg gtttattatc
     2401 ttgcctggct aatgcggcgt tgggctgatc ccgcgttccc gaaaaatttc ccgtggttaa
     2461 ccggggaaga ttactggctg cgacaaacgg cgacttttat agaacaggca aaagttctac
     2521 aagaaccccc tttacaatta acacctatgt attaatcgga gagagtagat catgaaaaag
     2581 atttggctgg cgctggctgg tttagtttta gcgtttagcg catcggcggc gcagtatgaa
     2641 gatggtaaac agtacactac cctggaaaaa ccggtagctg gcgcgccgca agtgctggag
     2701 tttttctctt tcttctgtcc gcactgctat cagtttgaag aagttctgca tatttctgat
     2761 aatgtgaaga aaaaactgcc ggaaggcgtg aagatgacta aataccacgt caacttcatg
     2821 ggtggtgacc tgggcaaaga tctgactcag gcatgggctg tggcgatggc gctgggcgtg
     2881 gaagacaaag tcactgttcc gctgtttgaa ggcgtacaga aaacccagac cattcgttca
     2941 gcatctgata tccgcgatgt atttatcaac gcaggtatta aaggtgaaga gtacgacgcg
     3001 gcgtggaaca gcttcgtggt caaatctctg gtcgctcagc aggaaaaagc tgcagctgac
     3061 gtgcaattgc gtggcgttcc ggcgatgttt gttaacggta aatatcagct gaatccgcag
     3121 ggtatggata ccagcaatat ggatgttttt gttcagcagt atgctgatac agtgaaatat
     3181 ctgtccgaga aaaaataatt caatgt
//
LOCUS       DADMKF010000270         3172 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.275, whole genome shotgun sequence.
ACCESSION   DADMKF010000270 DADMKF010000000
VERSION     DADMKF010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3172
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.275"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            530..2383
                     /locus_tag="JNC24_004073"
     CDS             530..2383
                     /locus_tag="JNC24_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HAY9160681.1"
                     /translation="MAFKHYDVVRAASPSDLAKRLTQKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVVVSGATEPEWYYVIVLAGQSNAMAYGEGLPLPDSYDAPHPRIKQLARRN
                     TVTPGGEVCVFNDIIPADHCLHDVQDMSTINHPRADLSKGQYGCVGQGLHIAKKLLPY
                     IPNNAGILLVPCCRGGSAFTQGTEGTFSESTGASQDSARWGVGKPLYQDLLFRTKAAL
                     QKNPKNVLLAICWMQGEFDMTNASYAQQPAAFLAMVQQFRADLAGLAAQCHGGSPASV
                     PWICGDTTYAWKQEHGTQYEVVYGAYKGKESQQIYFVPFMTDGSGVNTPTNNPSEDPD
                     IAGSGYYGSASRTNKNWVSSNRPTHFSSWARRGIIPDRMATAILNVAGRTLAFISGKA
                     PEIKPSPGGDTPSGPSEDASIRTISLLPTAGDAAAQGWSIKNGGVQLSDGVFKITKQS
                     NKAWSLTRPVDDAVSLLTRGGRLSCKFRLSGALTNNQFGLGIYLYTDVALPDVVAMTG
                     TGNPFLMSFFTQTTDGKLNLMHHKKAGNTKLGEFGNYSNDWLTLELVFTAGSATVTPK
                     LNGVAGPAFQVIKDSLTLGLNALTLTDITKNAAYGVEIESLVLEINAPASS"
     gene            2534..2749
                     /locus_tag="JNC24_004074"
     CDS             2534..2749
                     /locus_tag="JNC24_004074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAY9160682.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYLTNLYFKIREDRRKAARGE"
     gene            2754..3068
                     /locus_tag="JNC24_004075"
     CDS             2754..3068
                     /locus_tag="JNC24_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdfR family protein"
                     /protein_id="HAY9160683.1"
                     /translation="MTQNYELIVKGIRNFENKVTVTLALRDKKRFDGEIFDLDISLDR
                     VEGAALEFYEAAARRSIRQVFLDVAAGLCEGDELLPETRPCSEARYTIKINSSDNSIT
                     GC"
BASE COUNT          778 a          761 c          885 g          748 t
ORIGIN      
        1 ccaggcccat ccgctccggt gggccttttt actgcagaaa acaggttccc cgttaaatgc
       61 tatgttgctc acaattcagt aagttgacag ttgcctgtca gactgggcat ttgttaaaaa
      121 aatttcgcat ggtgaatccc cctgagcgga ggggcgactg gtgacggtat aatctctgat
      181 tatcaaaacg agaatgacgc gggtttagtg gcaccgggct gaactcaccg ggaggcaccc
      241 ggcaccatgt gcatgatgat acagatacgc ggctttagcc cctctccgga ggggttttct
      301 tgtgggcaaa aaaagcccgc gctgggagac gcgggcggca aggaataaac aataaaacgt
      361 gaagtaatat ttcagctggc gaataatacc ccatagtaat cactctgcgc aactgcgcgg
      421 tctttttcga attgcgggct gtcgtctctc ttctgccatt gtcctgtaac ttccggactt
      481 cagcccgctc cttattttac tcacaatatt atcccggccg ggaggattca tggcatttaa
      541 acactatgac gtggtcaggg cggcatcgcc gtcagacctt gcgaaacgac tgacacaaaa
      601 actgaaggag ggctggcagc cgtttggtag tccggtggcc ataacccctt ataccctgat
      661 gcaggcgatt gcagcagaag gtgatgtggt cgtcagtggt gcaactgagc cggagtggta
      721 ctacgtcatc gtactggccg ggcaatccaa tgccatggct tacggtgaag gtcttccgct
      781 tccggattca tacgatgcgc cccatccgcg cattaagcaa ctggcccgtc gtaacacagt
      841 gactcccggt ggtgaagtat gcgtatttaa cgacatcatt cctgctgacc attgtctgca
      901 tgatgttcag gatatgagta cgattaacca tccccgggct gacctgagca aagggcagta
      961 cggctgtgtc ggacagggct tacatattgc caaaaaactg cttccgtata tccctaataa
     1021 tgcggggatc ctgctggtac catgctgtcg tggtggttcg gcattcaccc agggcacgga
     1081 ggggacattc agcgagtcca cgggggccag tcaggattcg gctcgctggg gagtgggtaa
     1141 gccgttatat caggatctgc ttttccgcac gaaggcagca ttgcagaaaa acccgaaaaa
     1201 cgttttgctg gcgatatgct ggatgcaggg ggaattcgat atgacgaatg ccagttacgc
     1261 ccagcagcca gcagcatttc ttgcaatggt acagcagttc cgtgctgacc ttgccgggct
     1321 ggcggcgcag tgtcacggtg gaagtccggc atcagtcccc tggatttgtg gcgacacgac
     1381 atacgcgtgg aaacaagaac acggtacgca atatgaagtg gtatatggtg catataaagg
     1441 taaagaatcc cagcagattt attttgttcc ctttatgacc gatggtagcg gagttaatac
     1501 accgacaaac aacccgtcag aagatcctga tattgccggg tctggttatt acggttcggc
     1561 atcccgaacg aacaaaaact gggtatcatc aaatcgcccg acgcatttca gctcatgggc
     1621 gcgtcgtggc attattcccg atcgtatggc aactgctatt ctgaacgtag ccggtcgcac
     1681 cttagccttc attagtggta aggcaccgga aatcaaaccc tcgcccggcg gcgacacgcc
     1741 atcggggccg tctgaagatg catccatacg cacaatctcc ctgttgccga cagccggaga
     1801 cgctgctgcg cagggctgga gcattaagaa tggcggagtt cagttgtcag atggtgtatt
     1861 taagatcacc aagcagagca ataaagcctg gtccctgacg cgcccggtgg atgacgcagt
     1921 ctccctgctg acacggggtg gcagactgag ctgtaagttt cgactgtcag gcgcactgac
     1981 caacaaccag ttcggtctgg gaatttatct gtataccgat gtagcgttac ctgacgtcgt
     2041 ggcgatgacc gggactggta acccgttcct gatgtcgttc ttcacccaga ccacagacgg
     2101 caaactgaat ctgatgcatc acaagaaagc aggaaacaca aagttgggcg agttcgggaa
     2161 ttacagtaac gactggctga cgctggagct ggtgttcacc gccggcagtg ccacggttac
     2221 tccgaaactg aatggagtgg ctggcccggc attccaggtc ataaaagaca gtctgacact
     2281 ggggctgaat gcgctgacgc tgacggatat taccaaaaat gcagcgtatg gcgttgagat
     2341 agaaagtctg gtgctggaga taaatgcacc ggcatcatca taaaaagtga gccagccaaa
     2401 tggaaggtat cgttaaactc accggtagtg tcagtgggtc gtctgagatg cctgcatgag
     2461 ttatcagagc catcagtact taactggtgg ctttttttat tgttgtcagc ttccggataa
     2521 cgggagacgg ggtatgtacc agatggaaaa aatcacaaca ggtgtgtcat acaccacgtc
     2581 agcggtggga acgggctact ggttcctgca gttgctggac agggtttccc cgtctcagtg
     2641 ggcggcaata ggcgtgctgg ggagtctgct gtttgggctg ctgacatatc tgactaacct
     2701 gtatttcaaa atcagagagg accgtcgtaa ggctgcacgg ggagagtaat tcaatgactc
     2761 aaaactatga actgattgtg aaagggatcc gcaattttga gaataaagtt acggtaactt
     2821 tagcgttacg ggacaaaaaa cgctttgacg gtgaaatttt tgacctggac atctcgctgg
     2881 accgtgttga aggtgccgcg ctggagtttt atgaggcagc agccagaagg agcatcagac
     2941 aggtcttcct ggatgttgct gccgggttat gtgaagggga cgagctgttg ccagaaacgc
     3001 gcccctgttc agaggcgcgg tataccataa aaattaacag ttctgataac tcgattacgg
     3061 gttgttagct ttttgcagtt ggctttccag tatctttcat tggtagcatc ctgataaata
     3121 tccatgagcg caaaaatcaa atacggcctg tcagctgctg ttctggcgct ga
//
LOCUS       DADMKF010000271         3127 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.276, whole genome shotgun sequence.
ACCESSION   DADMKF010000271 DADMKF010000000
VERSION     DADMKF010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3127
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.276"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            <1..493
                     /locus_tag="JNC24_004076"
     CDS             <1..493
                     /locus_tag="JNC24_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000785587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HAY9160684.1"
                     /translation="ADPDYVIWAKEVQGINRAWTFRHWKGTGTVGVMVATNDPERPAP
                     DESVINAVRDHILPLAPVAGSGLYVFGATEKVIPMTIALSKDTPQIRTAIKAELNALM
                     LRDGVPEGRMYLSRISEAISLSAGEVAHRLIVPSSDIDLGETELPVLGEITWQAYDPA
                     RSK"
     gene            493..1083
                     /locus_tag="JNC24_004077"
     CDS             493..1083
                     /locus_tag="JNC24_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2313 domain-containing protein"
                     /protein_id="HAY9160685.1"
                     /translation="MDSLQDDYTKLLYGLMPPGPAWSDTDGVLDGLAPSLVRVHQRAD
                     ELVIEIDPGQSTELIERYEELYGLPDSCSPVGTQTLRQRQQRLEAKANVAGGINEQFF
                     LDQLEALGYTGVTIEQFQHLDASPDPEWGDRWRYFWRVTLPMDAGAQWQTCTDACNTP
                     IRTWGDTVAECVINKLCPSHTVVLFSYPDEDEDAQD"
     gene            1070..2110
                     /locus_tag="JNC24_004078"
     CDS             1070..2110
                     /locus_tag="JNC24_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000554725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY9160686.1"
                     /translation="MHRIDTLTAVKDKFGPGKNGFTDGNLRTGRLATWLNSAMWNAIQ
                     EEICGVIEKAGIELNKEEHDQLYKAILLLVGGAINEEALLIKNNLSDVEDRDEAVENL
                     GLKPTVDKAKNAVQRDGDTMTGELKIRGVNALRIFNEAFGLIFRRSEECLHLIPTSEG
                     QGENGDIGPLRPFTINLRTGEISMSHKVSVGGGSQVNGALGIGVQNALGGNSIVLGDN
                     DTGFKQNGDGLLDVYANSVHVLRFQSGSIQSNKAVNVTGRVTPSDYGNFDARYQQRNG
                     GVQDVRYGYEMYYPPGSNTVSWTFRSPSGHGLSGISISDTGRNSADNVNGVYYRPLQK
                     LINGTWYNVASI"
     gene            2170..2658
                     /locus_tag="JNC24_004079"
     CDS             2170..2658
                     /locus_tag="JNC24_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HAY9160687.1"
                     /translation="MTKQYDVTWLFSEDGKNWYEEQKNFASDTIKMVYTGDGRVVWVG
                     KDVTGIEPRNASVIEVPDITANRRITAPGYWFYRNDEFVFDYRLKAEDERDALLAQVS
                     ARTGEWEEDLLLGLISDEDREKLKAYRIYAKSLQAMDFSTITDKSSYNAIEWSVSPEG
                     SS"
     gene            2682..>3127
                     /locus_tag="JNC24_004080"
     CDS             2682..>3127
                     /locus_tag="JNC24_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY9160688.1"
                     /translation="MSVVISGALTDGAGIPMSGCQIILKSRVNTSEVVMRTRADVVTG
                     NNGEYSFEAQVGKYCVYLKRDWRDEYCVGDIAVYDDSKPGTLNDFLIAPDEGDLKPDV
                     VKRFEEMVAQAQQSAGAAAGNAQQTAQDVAAAAGYARAAEQAKNDI"
BASE COUNT          809 a          695 c          902 g          721 t
ORIGIN      
        1 cgcagaccct gattatgtta tctgggcgaa agaagttcag ggtattaacc gtgcatggac
       61 tttcagacac tggaaaggca ctggaacggt tggcgtgatg gtggcgacaa acgatccgga
      121 acgcccggcc ccggatgaaa gcgtgattaa cgcagtcagg gaccacatcc ttcctctggc
      181 acctgttgcc ggaagcggat tgtatgtatt cggtgccaca gaaaaagtca tcccgatgac
      241 gattgcatta tcgaaagaca caccgcagat caggactgca ataaaagcag agctgaatgc
      301 actgatgtta cgggatggtg tgccagaggg gcgtatgtat ctgtccagaa tcagtgaggc
      361 catcagtttg tctgctggtg aagtggccca ccggttaatc gtcccttcat cggatattga
      421 cctgggggag accgagcttc cggtgctcgg cgagatcacc tggcaggctt atgacccggc
      481 aaggagtaaa taatggattc gttacaggat gattatacaa agctgctgta cggactgatg
      541 ccgccggggc ctgcatggtc agataccgac ggtgtacttg acggtctggc accatcgctt
      601 gtgcgtgttc accagcgggc tgatgaactg gtgattgaaa tcgatcccgg tcagtccaca
      661 gagctgattg aacgttatga agaattgtat ggtttacctg attcctgttc ccctgtcgga
      721 acccagacat tacgccagcg tcagcaacgt cttgaagcaa aagccaatgt ggctggtggc
      781 ataaatgagc agttcttcct ggatcagctt gaggcgctgg gatataccgg cgtaacgatc
      841 gaacagttcc agcacttgga tgcaagcccc gatccggaat ggggagatcg ctggcgttat
      901 ttctggcgtg tgacgttgcc gatggatgcc ggtgctcagt ggcagacatg cacggacgcc
      961 tgcaacacac cgatccggac gtggggggat acggttgcgg agtgcgtgat taataaatta
     1021 tgtccgtcac acaccgtcgt tttattttcc tatccagatg aggatgaaga tgcacaggat
     1081 tgatacgctg actgcggtaa aagataagtt tggtccgggt aagaacggat ttactgacgg
     1141 aaatcttcgc acaggacgtc ttgctacctg gctgaacagt gccatgtgga atgccattca
     1201 ggaggaaatc tgtggtgtca ttgagaaggc cgggatagaa ctgaataaag aagaacacga
     1261 ccagctatat aaggctatat tattgctggt gggcggtgca attaacgaag aggcattgct
     1321 gataaaaaat aacctttcgg atgtggaaga cagggatgag gctgttgaaa acctcggatt
     1381 aaaacccacg gtggataagg caaaaaatgc cgttcagcgt gatggcgaca ccatgaccgg
     1441 agagctgaaa atccgtggtg ttaatgcgct gaggattttc aacgaagctt ttggcctgat
     1501 ttttcgtcgt tcggaagagt gcctgcacct tattcccacc agtgaaggtc agggggaaaa
     1561 tggcgatatt ggtcccctgc gcccgttcac cattaatctg cggacgggtg aaatatccat
     1621 gtcgcataaa gtgtctgttg gcggtggttc gcaggtcaat ggtgcgctgg gtatcggcgt
     1681 tcagaacgcc ctggggggga attcaattgt tcttggtgat aatgacaccg gattcaaaca
     1741 gaatggtgat ggcctgctgg atgtttatgc caatagcgtg catgtgttgc gttttcagag
     1801 tggcagtatc cagagtaata aagctgtaaa cgttacagga cgggtaacac cgtcagacta
     1861 cggaaacttt gatgcccgtt accagcagcg aaatggtggc gtgcaggatg tgcggtatgg
     1921 ttatgaaatg tattaccccc ccggcagtaa caccgtttcg tggacatttc gctcaccttc
     1981 gggacacggg ctgtcaggga tatcgatatc ggataccggc cgtaactcag cggataacgt
     2041 caacggcgtg tattaccgac cgctgcaaaa actgattaat ggcacctggt ataacgtagc
     2101 gagtatttaa caatgttgca tttaaaaaat attacggcag gtaatccgaa aaccgcggaa
     2161 taatatcagc tgacaaaaca gtatgatgtc acctggcttt tttcggaaga cggcaaaaac
     2221 tggtatgaag aacagaagaa ctttgccagt gacaccataa aaatggttta caccggagac
     2281 gggcgcgtgg tgtgggtcgg taaggatgtg acaggcattg aaccccgtaa cgccagtgtt
     2341 attgaagttc ctgatattac cgccaaccgc cgcattaccg cgccgggtta ctggttttac
     2401 cgcaatgatg aatttgtctt tgactacaga ctcaaagcgg aagatgagcg tgatgccctt
     2461 ctggctcagg tcagtgcccg gacaggggaa tgggaagaag acctgctgct ggggctaatc
     2521 agcgacgaag accgggaaaa actgaaagcg taccgtattt acgcgaaatc gctgcaggcg
     2581 atggatttca gcaccatcac tgataaatcc tcatataacg ccattgaatg gtccgtctct
     2641 ccggaaggtt cttcctgatt taatttatcg cgagaaaaac tatgtctgta gtgatatcag
     2701 gtgcgctgac tgatggcgca ggcatcccca tgtccggatg ccagattatt ctgaaatccc
     2761 gtgtaaacac ctcagaagtg gtgatgcgta ccagggctga tgtagtgacc ggaaataacg
     2821 gtgaatattc gtttgaggca caggtcggaa aatattgcgt gtatctgaaa cgggactggc
     2881 gcgacgagta ctgtgttggc gacattgctg tatacgacga ctcaaagccc ggcacgctga
     2941 atgactttct gattgctcct gatgagggcg acctgaaacc ggatgtcgtc aaacgctttg
     3001 aggaaatggt ggcgcaggcg cagcagagcg ccggggccgc agccggaaac gcacagcaga
     3061 cggcgcagga tgtggcggca gccgcaggtt atgcccgcgc agcagagcag gccaaaaatg
     3121 acattga
//
LOCUS       DADMKF010000272         3109 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.277, whole genome shotgun sequence.
ACCESSION   DADMKF010000272 DADMKF010000000
VERSION     DADMKF010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3109
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.277"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(<1..1255)
                     /locus_tag="JNC24_004081"
     CDS             complement(<1..1255)
                     /locus_tag="JNC24_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HAY9160689.1"
                     /translation="MAQFKVDDFLIELSFNSQKVLKGLEKAEKQTMQVASRIEKRLNK
                     SFRVNPTPLNDSLKLMERNVDKTVSKIEQRLKNTRAFKIKTEIEDTLKPLRQPRQPRI
                     SGSRAITAAYSANMSKLRDFNPMLEKYFKSQYYSLSSKSKTLGNETFNRELAKLNQSL
                     RETLSKFNKTTSKNNHSENASNSLDVLATSAIKAGTAIYSFQTALEAYRRIMEVGLKK
                     EASQRAAQFVLGDEGAKRATEFVKNLADSSGVDQIETLSSFAKFSAGAGDMNADQKES
                     LFSNVIGTSRLMGLSTDEINGILKAFEQMASKGKIQAEELRGQLGDRMAGAFQLFARS
                     LGMTTEELDKAMKDGKVLSKDVFPKVSAEMGRMIDKAGGWEKIINSTQTQLGRLSNSW
                     NNNLALMFDGSQEGLTDFTRSLTNLL"
     gene            complement(1412..1621)
                     /locus_tag="JNC24_004082"
     CDS             complement(1412..1621)
                     /locus_tag="JNC24_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9160690.1"
                     /translation="MFEERLETIRSVCENLKLQTKPALRIKNKRQVITSHKPKTRKIP
                     KWCIDRIPSDAQIIGETELHYLVRH"
     gene            complement(1644..2354)
                     /locus_tag="JNC24_004083"
     CDS             complement(1644..2354)
                     /locus_tag="JNC24_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000180371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY9160691.1"
                     /translation="MTNDLYINGKKVDSFGDWSPQGEHPLHTIQRQEHDKRIELEQEL
                     KMSPKVISFVSPEPQEMPDICKTEALFELEREYYPALKSQRLRLDVAYDAVKNFEETS
                     KPSEYDIAYEIKSNPFIYYEGNFNDGFGTAIEDVPKVLQSIPEGFRLIDVRQALRGSG
                     QFILMTDKTDEEILQLAEANLTKHYSGKLDKLKQKLKKELDTMKELVSEYESQKKIAL
                     MADIEQLTKISQKYSKAV"
     gene            complement(2870..>3109)
                     /locus_tag="JNC24_004084"
     CDS             complement(2870..>3109)
                     /locus_tag="JNC24_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9160692.1"
                     /translation="DLDKLLKLMAENSEEIEDFIQEIETGEPRFMFPVTDSNMKRLII
                     EETNKYSVSPEKYLKAAIAILYSDNILVTDSKVVH"
BASE COUNT          911 a          654 c          507 g         1037 t
ORIGIN      
        1 taagtaaatt agtcagtgac cttgtaaagt ctgttaaacc ttcctgtgaa ccatcgaaca
       61 ttaaggccag attattattc catgaattac ttaagcggcc taactgcgtc tgagtagagt
      121 taataatctt ttcccatcca ccagctttat caatcatcct tcccatttca gcggatactt
      181 taggaaaaac atctttagaa agaaccttac cgtctttcat ggccttgtct aattcttccg
      241 tagtcatccc aagacttctg gcaaataact gaaaagcacc tgccattctg tcacctaatt
      301 gaccgcgtag ctcttcagcc tgaatctttc ctttagatgc catctgctcg aaagctttta
      361 aaatcccgtt aatttcatcg gtgcttagcc ccattaatct ggatgtacca atcacattag
      421 aaaataagga ttctttctgg tctgcattca tatcaccagc accagcggaa aacttagcaa
      481 aactgcttaa tgtttcaatc tggtctacac cactactatc agcaagattc ttaacaaatt
      541 ctgtagctcg tttagcacct tcatcaccta aaacgaattg tgccgctcgt tgtgatgctt
      601 cttttttcag accaacctcc ataatccgcc tgtaagcttc taaggctgtc tgaaaactat
      661 aaatggctgt acctgctttg attgcactcg tagctaatac atccagacta tttgaagcat
      721 tttcactgtg attatttttg ctggtggtct tgttaaactt agaaagtgtt tcacgaagag
      781 actgatttaa ctttgctaac tcacgattaa aagtttcgtt tccaagtgtc ttggatttac
      841 tgcttaagct gtaatactgg cttttaaaat acttctcaag cataggatta aagtcacgaa
      901 gcttgctcat attcgctgag taagctgctg tgattgctct gcttccgcta attcgtggct
      961 gtcttggttg tctaagtggc tttaaagtat cctcaatttc agttttaatc ttaaacgctc
     1021 tagtgttttt aagacgctgc tcaatcttgg aaacagtttt atcgacattt ctttccatta
     1081 gtttgagaga atcatttaaa ggcgtaggat taactctaaa cgacttattc aatcgcttct
     1141 caattcgtga tgctacctgc atcgtttgtt tctcggcttt ttccaagcct tttaaaactt
     1201 tctgagagtt aaaacttaac tcaatcagaa aatcatcaac tttaaattgt gccattcata
     1261 tttttcacct ctttaagcgt gttgtacgta aattctattt ttcagtgtgg gtattagctt
     1321 tacagatttc acgtagcaca catacaaaaa aagggagagc taccgatgtc actcgataat
     1381 ctctccccgt atttacccaa ccaattcttt tttaatgtcg gacaagataa tgtaattccg
     1441 tttcacctat gatttgagca tcagaaggaa tacggtcaat acaccacttt ggaatcttgc
     1501 gtgtttttgg tttgtgtgag gtgataactt gacgtttgtt tttaattctt aaggctggct
     1561 tagtttgtaa ctttaagttt tcacaaacag accgaatagt ctctaaacgt tcttcaaaca
     1621 ttaatcacct ctttaattca taatcaaaca gccttgctat acttctgaga aatcttcgtt
     1681 aactgctcaa tatcagccat caaagcaatc ttcttctgcg actcatattc actaacaagt
     1741 tctttcatag tatcaagttc ttttttaagc ttttgcttta acttatccag tttaccggaa
     1801 taatgcttag tcaaattagc ttcagctaac tgaagaatct cttcatctgt tttatcagtc
     1861 atcagaataa attgaccaga accccttaat gcttgtctga catcaattaa ccggaaccct
     1921 tcagggatac tttgaagaac tttaggaacg tcttcaatcg ctgtaccaaa accatcatta
     1981 aagtttcctt cataatagat aaacgggttt gacttaatct cgtaagcaat atcatattct
     2041 gaaggtttcg aggtttcttc gaagttctta acagcatcgt aagcgacatc aagtctaaga
     2101 cgctgtgatt ttaaagctgg gtaatattca cgctcaagct caaatagtgc ttcagttttg
     2161 cagatatcag gcatttcttg aggctcagga gacacaaagg aaattacctt tggtgacatc
     2221 tttagctctt gctcaagctc aatgcgcttg tcatgctctt gacgctgaat agtatgtagc
     2281 ggatgttcac cctgtggtga ccagtctccg aagctatcaa cctttttacc gttaatatat
     2341 aaatcgtttg tcattataat ttcctctaag tgtggttatt tcttaataga gcaccttcaa
     2401 cgccaataca cttagaagat gacttaacga tttattaaag atgctctatt aagaaaagcc
     2461 ctaaacataa tctgtgaagg gctttggagg tttgcttata acgttaacga agtttcagga
     2521 aaactttctt taaaagctct gtttaaaaac tcttaaagaa agccctaact catgattagg
     2581 gcaaagctgt aaaggattca ataatgaatg aacaaagact ttttgagata gcctctcaga
     2641 ccaacctgaa aggctaggac gaaaagcatt gcacctttcc aactcagaat atccattcat
     2701 cggattattg aaagaaaaaa taagagagaa ttgctctccc ccatatatac aattatgtca
     2761 tgctcaaggt tgtttgtcaa cagcctttaa cagattggta acatttatac tttaatagat
     2821 gcacctgcaa tcataccaat caatacaaaa cagataatca ccaattccat taatgcacca
     2881 ccttcgaatc cgtcaccaga atattatcgc tatacaaaat tgctatagcc gccttgagat
     2941 atttttcagg gctaacgctg tatttgttcg tttcttcgat aataagacgc ttcatgtttg
     3001 agtcggtaac aggaaacata aaacgcggtt caccagtttc aatttcctga ataaagtctt
     3061 caatttcttc tgagttttca gccattagct ttagtaattt atcaaggtc
//
LOCUS       DADMKF010000273         3045 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.278, whole genome shotgun sequence.
ACCESSION   DADMKF010000273 DADMKF010000000
VERSION     DADMKF010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3045
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.278"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            <1..172
                     /locus_tag="JNC24_004085"
     CDS             <1..172
                     /locus_tag="JNC24_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HAY9160693.1"
                     /translation="AKALMAQYRVPVVVEVILERVTNISMGSELDNVMEFEDIADNAV
                     DAPTETCFMHYE"
     gene            185..1036
                     /gene="hyi"
                     /locus_tag="JNC24_004086"
     CDS             185..1036
                     /gene="hyi"
                     /locus_tag="JNC24_004086"
                     /EC_number="5.3.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000943555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HAY9160694.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAIRYARALGNKKI
                     NCLVGKTPAGFSSEQIHATLVENLRYAANMLMKEDILLLIEPINHFDIPGFHLTGTRQ
                     ALKLIDDVGCCNLKIQYDIYHMQRMEGELTNTMTQWADKIGHLQIADNPHRGEPGTGE
                     INYDYLFKVIENSDYNGWVGCEYKPQTTTEAGLRWMDPYFLNRVVATGKVMKPVLAQR
                     QKVVSPP"
     gene            955..2133
                     /gene="allB"
                     /locus_tag="JNC24_004087"
                     /pseudo
     CDS             955..2133
                     /gene="allB"
                     /locus_tag="JNC24_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415045.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
     gene            2190..>3045
                     /locus_tag="JNC24_004088"
     CDS             2190..>3045
                     /locus_tag="JNC24_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil/xanthine transporter"
                     /protein_id="HAY9160695.1"
                     /translation="MFNFAVSRESLLSGFQWFFFIFCNTVVVPPTLLSAFQLPQSSLL
                     TLTQYAFLATALACFAQAFCGHRRAIMEGPGGLWWGTILTITLGEASRGTPINDIATS
                     LAVGIALSGVLTMLIGFSGLGHRLARLFTPSVMVLFMLMLGAQLTTIFFKGMLGLPFG
                     IADPYFKIQLPPFALSVAVMCLVLAMIIFLPQRFARYGLLVGTITGWLLWYFCFPSSH
                     SLSGELHWQWFPLGSGGALSPGIILTAVITGLVNISNTYGAIRGTDVFYPQQGAGNTR
                     YRRSFVATG"
BASE COUNT          695 a          711 c          836 g          803 t
ORIGIN      
        1 ggcgaaagcc ttaatggcgc aatatcgggt accggtagtc gtggaagtta ttcttgagcg
       61 tgtgaccaat atttcgatgg gcagcgaact ggataacgtc atggaatttg aagatatcgc
      121 cgataacgca gtggacgcac cgactgaaac ctgcttcatg cactatgaat aagggagata
      181 aataatgtta cgtttctctg ctaatttatc gatgttattt ggtgaatatg attttctcgc
      241 ccgttttgag aaagctgcgc agtgtggttt tcgcggcgtt gaatttatgt ttccttatga
      301 ctacgacatt gaagaattaa aacaggtgct ggcgagtaat aaactcgaac atacgctgca
      361 caatctaccg gcgggtgact gggcggcggg ggagcgcggt attgcctgta ttcctggccg
      421 tgaagaagag tttcgggatg gcgtagcagc agcgattcgt tatgcccgtg cgctggggaa
      481 taaaaaaatt aactgtctgg tcggtaaaac gccggctggt ttcagcagtg aacagattca
      541 cgcaacgctt gtagaaaacc tgcgttatgc cgcgaatatg ctgatgaaag aagatatttt
      601 attactgatt gaacctatta accattttga tattcctggt ttccatctca ccggaactcg
      661 gcaggcgctg aaattgattg atgatgttgg ttgctgcaat ttgaaaattc agtatgacat
      721 ttatcacatg cagcggatgg aaggtgaatt aaccaacacc atgactcagt gggccgataa
      781 aattggtcac ctgcaaattg ctgataatcc gcatcgcggc gaaccgggga ccggagaaat
      841 taattatgat tatctcttta aggtaatcga aaattctgac tacaacggtt gggttgggtg
      901 tgaatataaa ccccaaacca ccacggaagc cggtttacgc tggatggatc cgtactttct
      961 gaaccgggtc gtagccactg ggaaggttat gaaaccggta ctcgcgcagc ggcaaaaggt
     1021 ggtatcacca ccatgatcga aatgccgctc aaccagctgc ctgcaacggt tgaccgcgct
     1081 tcaattgaac tgaagttcga tgccgctaaa ggcaagctga ctatcgatgc ggcacaactc
     1141 ggtggcctgg tgtcttacaa catcgaccgt ctgcatgagc tggatgaagt gggcgttgtc
     1201 ggcttcaaat gcttcgttgc gacctgtggc gatcgcggta tcgacaacga cttccgtgat
     1261 gtaaacgact ggcagttctt caaaggtgcg cagaagctgg gcgaactggg gcagccggtg
     1321 ttggtgcact gcgaaaacgc gctgatctgt gacgcaccgg gcgaagaagc gaaacgtgaa
     1381 ggtcgcgtaa ctgcccatga ctatgtggct tcgcgtccgg tatttaccga agtggaagcg
     1441 attcgccgcg tgctgtatct ggcgaaagtt gccggttgcc gtctgcacgt ttgccatatc
     1501 agcagcccgg aaagtgttga agaagtgact cgtgcacgtc aggaaggtca ggatgttacc
     1561 tgtgaatcct gcccgcatta ctttgtactg gataccgatc agttcgaaga aatcggtact
     1621 ctggcgaagt gttcaccgcc gatccgcgat ctggaaaacc agaaaggcat gtgggaaaaa
     1681 ctgtttaacg gtgaaatcga ctgcctggtt tccgaccact caccatgccc tccggaaatg
     1741 aaagccggca acatcatgga agcatggggc ggtattgccg gtctgcaaaa ctgtatggac
     1801 gtgatgttcg atgaagcggt acagaaacgc ggaatgtctc tgccaatgtt cggcaaatta
     1861 atggcgacta acgcagcaga tattttcggt ctgcagcaaa aaggccgtat cgccccagga
     1921 aaagatgccg acttcgtctt cattcagccg aatagcagct atgttcttac caatgacgat
     1981 ctggaatatc gccacaaagt cagcccgtat gttggccgta ccattggcgc gcgtatcacg
     2041 aaaaccatct tacgtggtga tgtgatttac gacattgaac agggcttccc tgttgcgccg
     2101 aaaggtcaat ttatccttaa acatcagcag taatctggcc cctgcaatgc ccgtccttgc
     2161 ggcgggcatt ctccggttaa ggtgtgttta tgttcaattt tgcagtcagc cgcgaaagcc
     2221 tgttatcagg atttcagtgg tttttcttta ttttttgcaa cacggttgtg gttcctccta
     2281 cgctactttc tgcttttcag ttgccgcaaa gtagcctgct tacgctcacg caatatgctt
     2341 ttcttgctac cgcactggcc tgcttcgctc aggcgttctg cggtcatcgt cgcgctatta
     2401 tggaagggcc gggtggcctg tggtggggaa ccatccttac tatcaccctt ggcgaagcat
     2461 cgcgcgggac accgatcaac gatatcgcta ccagtctggc ggtggggatt gcactctccg
     2521 gcgtgctgac gatgttgatt ggttttagcg gattaggcca tcgcctggca cggttattta
     2581 cgccgtcggt gatggtcttg tttatgttga tgctgggcgc acagctgacc actatctttt
     2641 tcaaaggtat gctcgggctg ccgtttggca tagccgaccc atattttaaa attcagttac
     2701 caccgttcgc gctctcggtg gcggtgatgt gcctggtact ggcgatgatt atctttctgc
     2761 cgcaacgttt tgcccgttat ggcctgctgg tcggcaccat aaccggctgg ttgttgtggt
     2821 acttttgctt tccttcttcg cattcgctct ccggtgagtt gcactggcag tggttcccgc
     2881 tcggcagtgg cggtgctttg tcgccgggaa ttattctgac ggcggtgatt acaggtctgg
     2941 taaatatcag caatacctac ggtgcgattc ggggcacgga tgttttttat ccgcagcagg
     3001 gcgcggggaa tacgcgttat cgtcgcagct ttgtggcgac cggat
//
LOCUS       DADMKF010000274         3040 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.279, whole genome shotgun sequence.
ACCESSION   DADMKF010000274 DADMKF010000000
VERSION     DADMKF010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3040
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.279"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            2..1197
                     /gene="bluF"
                     /locus_tag="JNC24_004089"
                     /pseudo
     CDS             2..1197
                     /gene="bluF"
                     /locus_tag="JNC24_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415681.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="blue light-responsive regulator BluF"
     gene            1402..2133
                     /locus_tag="JNC24_004090"
     CDS             1402..2133
                     /locus_tag="JNC24_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAY9160696.1"
                     /translation="MAYYSIGDVAERCGINPVTLRAWQRRYGLLKPQRSEGGHRLFDE
                     EDIQRIEEIKRWISNGVPVGKVKALLETTSQDTEDDWNRLQEEMMSILRMANPAKLRA
                     RIISLGREYPVDQLINHVYLPVRQRLVLDHNTSRIMSSMFDGALIEYAAASLFEMRRK
                     PGKEAILMAWNVEERARLWLEAWRLSLSGWHISVLADPIESPHPELFPTQTLIVWTGM
                     APTRRQNELLQHWGEQGYKVIFHAP"
     gene            2354..2758
                     /locus_tag="JNC24_004091"
     CDS             2354..2758
                     /locus_tag="JNC24_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 domain-containing protein"
                     /protein_id="HAY9160697.1"
                     /translation="MDQVVVFQKMFEQVRKEQNFSWFYSELKHHRIAHYIYYLATDNI
                     RIITHDDTVLLLRGTRNLLKVSTTKNPAKIKEAALLHIYGKSTFREYCSTLAGAGVFR
                     WVTDVNHNKRSYYAIDNTLLYIEDVENNKPLI"
     gene            2811..2921
                     /locus_tag="JNC24_004092"
     CDS             2811..2921
                     /locus_tag="JNC24_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9160698.1"
                     /translation="MGIKVQRLSLWMINTVLLLLPINNHQANTIKLIFEM"
BASE COUNT          904 a          634 c          644 g          858 t
ORIGIN      
        1 aatgcttacc acccttattt atcgtagcca tatacgtgac gacgaacctg tcaaaaaaat
       61 cgaagaaatg gtttcgatag caaatcgcag gaacatgcag tctgacgtaa cagggatctt
      121 actgtttaat ggttctcatt ttttccagct tctggaaggt ccggaagaac aggttaaaat
      181 gatatatcgg gctatatgcc aggatccacg gcactataat attgttgagc tgctgtgcga
      241 ttacgcgcct gctcgccgtt ttggcaaagc gggaatggaa ttatttgatt tgcgcctgca
      301 cgagcgagat gacgttttac aggccgtatt cgacaaaggc acatcaaaat ttcagctaac
      361 ttatgatgac agagcgctac aattttttcg tacttttgtc cttgcaaccg aacaatcaac
      421 ctatttcgag atccctgccg aagactcctg gctttttatc gctgacggat ctgataaaga
      481 acttgattcc tgtacccttt caccaactat aaacgaccac tttgcctttc atcccttatc
      541 gcggcggata atcgcttttg aagccattgt gcaaaaaaat gaagcccatc agccatagcg
      601 gttgggcagc gtaaagacgg ggaaatctac acagcggatc tcaaaagtaa ggcgcttgca
      661 ttcgcgatgg cacacgcact tgagctcggt gataaaatga tttcaatcaa tctattacct
      721 atgaccctgg ttaacgaacc tgacgcagtc tcttttttac ttgatgaaat aaaggccaat
      781 gctctggtgc ctgaacaaat catcgttgaa tttactgaaa gtgaagttat atctcggttt
      841 gatgagtttg cagaagcgat taaatcgctt aaggctgtcg gtatcagtgt agcaattgat
      901 cattttggcg caggttttgc cggtttgtta ctcctgtcac gcttccagcc tgacagaatt
      961 aaaatcagtc aggaattgat taccaatgtt cataaaagcg ggccacggca ggcaattatt
     1021 caagcgatca taaaatgctg tacatcactt gaaattcaag tcagtgctat gggcgtggca
     1081 acaccagaag agtggatgtg gcttgaatcc gcaggaattg agatgtttca gggagatctg
     1141 tttgctaaag ctaaattgaa tggtatccct tccatagcgt ggccagagaa aaaataattt
     1201 tcagcacatt ctttcacatg atttcagtaa atcatcgtga aaatgaatac gttatgttta
     1261 aatttaatcc acattaaata taatgtttca tccaatttag ctgaaatctt atttatactt
     1321 tgtacaattt tagcgtatat tgtacaaagt attattgggt cgtgtacagg cggcggagat
     1381 ttatgaccgc aaggaggaat tgtggcttat tacagcattg gtgatgttgc tgaacgttgc
     1441 gggattaatc ctgtcactct ccgggcctgg caacgccgct acggtttgtt aaaaccacag
     1501 cgcagtgaag gcggacaccg actctttgat gaagaagaca tacaacgcat cgaagagatc
     1561 aagcgttgga taagtaatgg cgtccctgta ggcaaagtta aagcattact ggaaaccacc
     1621 agccaggata cggaagatga ctggaaccgc ctgcaagaag agatgatgtc aattcttcgt
     1681 atggctaatc ctgccaaact acgcgcgaga attatttcac tgggtcgaga gtacccagta
     1741 gatcaattga ttaatcatgt ttaccttcct gttcgccagc gtctcgtgct tgatcacaac
     1801 acctcccgca ttatgagcag tatgtttgac ggcgcattaa ttgaatatgc agcagcctca
     1861 cttttcgaaa tgcgccgtaa gcccggtaaa gaagccattc tgatggcgtg gaatgttgaa
     1921 gagagagcac gattgtggct ggaagcatgg cgtttatcat tgtcaggatg gcacatttct
     1981 gttcttgctg atcccattga atcgccgcac ccggaactgt tcccgacgca aacattgatt
     2041 gtctggacag gcatggcacc aacgagaagg caaaacgaac ttttacaaca ttggggtgag
     2101 caaggttata aagtcatctt tcatgccccc taatttcacc tttatcccgc cccttagaca
     2161 gaatgttata atctgaaccc acaacagcat ttgtaccgga gctggtccgt tgcaaaatgc
     2221 tcaccggaag aattcaggta atccctctat cacactctgt acatatcaca gccaaaggac
     2281 agaccagatt gaagtggtaa aaccgatcgt gcacactacg cagtctcaca actaacaaga
     2341 ggttattatc ctgatggatc aggtcgttgt ttttcaaaag atgtttgagc aagtgagaaa
     2401 agagcaaaac ttctcctggt tttattcaga attaaaacat caccgtattg cacattacat
     2461 ttattatctg gctacggata acatcagaat tattactcac gatgacacgg ttttgttatt
     2521 aagaggaacc aggaacctgt taaaagttag tacgaccaag aaccctgcta aaataaaaga
     2581 ggccgcattg cttcatattt acggaaaatc tacatttcgg gaatactgtt caacactggc
     2641 aggcgcgggc gttttccgtt gggttactga tgttaatcat aacaaacgca gttactacgc
     2701 cattgataat acgcttttat atattgaaga tgtagaaaat aataaaccat taatctagct
     2761 aaagttggat gcttaagaaa tgcttcataa ttcagtaagg cattagcata atgggaataa
     2821 aagtacagag actatcccta tggatgataa atactgtcct tttattgcta cccataaata
     2881 atcaccaggc caatactatc aaattgatat ttgaaatgtg atcacttgac tttctatacg
     2941 ttattttata acggttaata tatttataaa aacaacgggt gtgccatacg cccgtttcaa
     3001 tacttaacgc acatgtgttt tggtttagtc atcatccagt
//
LOCUS       DADMKF010000275         3028 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.280, whole genome shotgun sequence.
ACCESSION   DADMKF010000275 DADMKF010000000
VERSION     DADMKF010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3028
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.280"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(<1..370)
                     /gene="quuD"
                     /locus_tag="JNC24_004093"
     CDS             complement(<1..370)
                     /gene="quuD"
                     /locus_tag="JNC24_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein QuuD"
                     /protein_id="HAY9160699.1"
                     /translation="MRDIQMVLERWGAWAANNHEDVTWSSIAAGFKGLITSKVKSRPQ
                     CCDDDAMIICGCMARLKKNNSDLHDLLVDYYVVGMTFMSLAGKHCCSDGYIGKRLQKA
                     EGIIEGMLMALDIRLEMDIVV"
     gene            complement(456..596)
                     /locus_tag="JNC24_004094"
     CDS             complement(456..596)
                     /locus_tag="JNC24_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcG family protein"
                     /protein_id="HAY9160700.1"
                     /translation="MMFEFYMAELLRHRWGHLRLYRFPGSVLTDYRILKNYAKTLTGA
                     GV"
     gene            complement(593..955)
                     /gene="rusA"
                     /locus_tag="JNC24_004095"
     CDS             complement(593..955)
                     /gene="rusA"
                     /locus_tag="JNC24_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RusA"
                     /protein_id="HAY9160701.1"
                     /translation="MNTYSITLPWPPSNNRYYRHNRGRTHISAEGQAYRDNVTRIIKN
                     AMLDIGLAMPVKIRIECHMPDRRRRDLDNLQKAAFDALTKAGFWLDDAQVVDYRVVKM
                     PVTKGGRLELTITEMGNE"
     gene            complement(952..1242)
                     /locus_tag="JNC24_004096"
     CDS             complement(952..1242)
                     /locus_tag="JNC24_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HAY9160702.1"
                     /translation="MADLRKAARGRECQVRIPGVCNGNPETSVLAHIRLAGLCGTGIK
                     PPDLIATIACSACHDEIDRRTYFVDAEYAKECALEGMARTQVIWLKEGVIKA"
     gene            complement(1235..1405)
                     /locus_tag="JNC24_004097"
     CDS             complement(1235..1405)
                     /locus_tag="JNC24_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9160703.1"
                     /translation="MATPLIRVMNGHIYRVSNRRKRKPELKPSEIPTLLGYTASLVDK
                     KWLRLAARRNHG"
     gene            complement(1405..1860)
                     /locus_tag="JNC24_004098"
     CDS             complement(1405..1860)
                     /locus_tag="JNC24_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9160704.1"
                     /translation="MTLPVDGIKLHRGNFAAIGQQIQPLLDAGQCLRLQVKPWREKRS
                     LSQNALSHMWYTEISEYLIARGKTFATPEWVKDAMKHTYLGYESKDRVDVVSGEVTTV
                     QSLRHTSELETGEMYIFLCKVEAWAMNIGCHLTIPQSCEYQQLRDKQEA"
     gene            2125..2502
                     /locus_tag="JNC24_004099"
     CDS             2125..2502
                     /locus_tag="JNC24_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000182282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9160705.1"
                     /translation="MTNKENKDKYINKFKKELSKYIQPTAGVDIQLFNSKDGGGVIKA
                     TLNRSGKRKSSIAGNFTKLGEAITSSGQRVFGGDLSNVNFYGTNTIFDGDTIFLIKSP
                     DSEEWSSQKASKDVEGIVFGGKK"
BASE COUNT          831 a          713 c          648 g          836 t
ORIGIN      
        1 taacaacgat atccatctct aaccggatat ctaatgccat taacatccct tcaattatgc
       61 cctcagcctt ctgtaacctt ttcccgatat aaccatcaga gcagcaatgc ttacctgcca
      121 gtgacatgaa tgtcataccg actacataat aatctactaa taaatcgtgc aaatcgctgt
      181 tgttcttttt cagacgggcc atgcacccgc aaataatcat cgcgtcatcg tcacaacatt
      241 gcgggcgaga ttttactttt gaagtaatta atcccttaaa accggcggca atggacgacc
      301 aggtcacatc ttcatgatta ttagccgccc acgctcccca acgctcaaga accatctgaa
      361 tatcacgcat caacttactc cacaaaaatc agaccagaac gccaattaca agcaaaaatc
      421 aacaaaacag tattagttga ttgttatctc tgacttcata ctcctgctcc tgtcagggtt
      481 ttggcgtaat tcttcagtat tcggtaatcg gtcaaaacag aaccggggaa acgatataag
      541 cgcagatgcc cccagcggtg gcgaagaagt tctgccatat aaaactcaaa catcattcat
      601 tccccatttc ggtgatggtc agttccagcc tcccaccttt ggtaacaggc atcttcacaa
      661 cgcggtaatc aacgacctga gcatcatcca gccagaaacc tgctttggtg agtgcgtcaa
      721 aagcggcttt ttgcagatta tccaggtcac ggcgacggcg atccggcatg tggcactcaa
      781 tacggatttt cactggcata gccaggccga tatccagcat tgcgttttta atgattcggg
      841 tgacgttatc gcggtatgcc tgcccttctg cgctgatgtg tgtgcgcccg cgattatggc
      901 ggtaatagcg gttattgctc ggaggccagg gtaatgtgat gctgtaggta ttcacgcctt
      961 aataaccccc tctttcagcc agataacctg cgttctcgcc ataccttcca gcgcgcattc
     1021 ttttgcatac tcagcatcga caaaatatgt gcggcggtcg atttcgtcgt gacaggcaga
     1081 acatgcaatg gtggcaatca ggtctggcgg tttgataccg gtaccgcaca atccagccag
     1141 ccggatatgt gccagtactg acgtttcagg attgccatta catacgccag ggattcttac
     1201 ctggcattcc cgaccacgcg ctgcttttct caaatcagcc atgattcctc cttgctgcca
     1261 gtcgcaacca ttttttatca accaggctag cggtatatcc gagcagtgtt ggtatttcgg
     1321 atggcttcag ctcaggctta cgcttacgac gatttgatac tctgtagatg tgcccgttca
     1381 tgacacgaat aagcggtgta gccattacgc ctcctgctta tcgcgcagct gctggtattc
     1441 acagctctgc ggaattgtca ggtggcagcc gatattcatc gcccaggctt cgactttgca
     1501 caggaagatg tacatctcgc cggtttccag ctctgacgta tggcggaggg attggacggt
     1561 ggtgacctcg ccggacacga catctacacg gtctttgctt tcgtagccga gataggtgtg
     1621 cttcatcgcg tctttgaccc actcaggcgt agcgaaggtc ttgccgcggg cgatgagata
     1681 ttcgctgatc tccgtgtacc acatgtggct gagcgcgttc tgcgacaggc tgcgcttctc
     1741 gcgccacggc ttaacctgca ggcgtaagca ttgcccggca tccagcaatg gctgaatctg
     1801 ctggccgatg gccgcgaagt taccgcgatg gagtttgatg ccgtctactg gcagagtcat
     1861 acggcctccc taacggaaac cgcagaatgc agaaaatcgc aggtgcattt ctgcatctgt
     1921 gacaaggtga tgagttcaga ttgtggtcgc atttaagtcc ccttaaatgc gcagaagtca
     1981 ccggagttgt tcaggctccg atgagaggat tatggcggat tgattacgat aaatcaaaac
     2041 tatcttgaag ccaaaacagt gatcttcgct ttaaaaacct tgtatacttt cacctaatca
     2101 cagccagaac tatggatcta accaatgacc aacaaagaaa acaaagataa atatattaac
     2161 aagttcaaga aagagctttc aaaatatata caaccaactg ctggagtgga cattcaatta
     2221 tttaactcta aggatggtgg tggtgtaatt aaagccactt tgaatagaag tggaaaaaga
     2281 aaatcatcta ttgctggtaa ttttactaaa ttaggtgagg caattacctc gagtgggcag
     2341 agggtttttg gtggtgatct atcaaatgtg aatttttacg gaacaaatac gatttttgat
     2401 ggagatacaa tatttttaat taaatcacct gattcagagg agtggtcatc acaaaaagct
     2461 tccaaagacg tagagggcat cgtatttgga gggaaaaaat gattaacatt actactaagg
     2521 atgcactcaa gtcagtggtg aagtaagaga tctagtaatt cttcttgaaa ctgatggaaa
     2581 aacggtgtct gattatttgc agccaatccc tcccaaaaaa gatacacact ggagcatgat
     2641 tttcattccg gtagcaattt atatactact taatcttgta tacattattg ctcacccatt
     2701 atttcctgaa aaatatctaa aaacatcaac atctatattt ctattcattt ccttcatatt
     2761 aagtgcaata gtagcatatt ttgttttcca taagcataaa tctgccactc tatccatatg
     2821 cagcttagtg ttcttagtgt taattgtagg tgctaacgta gggtttatca gttacagcga
     2881 cctaacacaa aaagcgacgc aaaaattaga tagtttgtct aaataattat gttgccatgc
     2941 gaagtatgca tggctgcatg tctgccttcc atttaaaatg ggctaagacc tataacccta
     3001 aatattattc tttattatct tctttacc
//
LOCUS       DADMKF010000276         2992 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.281, whole genome shotgun sequence.
ACCESSION   DADMKF010000276 DADMKF010000000
VERSION     DADMKF010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2992
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.281"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            18..1076
                     /locus_tag="JNC24_004100"
     CDS             18..1076
                     /locus_tag="JNC24_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000035736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HAY9160706.1"
                     /translation="MAQAGIRPLVTLSHYEMPYGLVKNYGGWANRAVIDHFEHYARTV
                     FTRYQHKVALWLTFNEINMSLHAPFTGVGLAEESGEAEVYQAIHHQLVASARAVKACH
                     SLIPEAKIGNMLLGGLVYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYMQRFFR
                     DHNITIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPNPHLKS
                     SEWGWQIDPVGLRVLLNTLWDRYQKPLFIVENGLGAKDSVEADGSIQDDYRIAYLNDH
                     LVQVNEAIADGVDIMEYTSWGPIDLVSASHSQMSKRYGFIYVDRDDNGEGSLTRTRKK
                     SFGWYAEVIKTRGLSLKK"
     gene            1162..2778
                     /locus_tag="JNC24_004101"
     CDS             1162..2778
                     /locus_tag="JNC24_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HAY9160707.1"
                     /translation="MFRRNLITSAILLMAPLAFSAQSLAESLTVEQRLELLEKALRET
                     QSELKKYKDEEKKKYTPATVNRSVSTNDQGYAANPFPTSSAAKPDAVLVKNEEKNASE
                     TGSIYSSMTLKDFSKFVKDEIGFSYNGYYRSGWGTASHGSPKSWAIGSLGRFGNEYSG
                     WFDLQLKQRVYNENGKRVDAVVMMDGNVGQQYSTGWFGDNAGGENFMQFSDMYVTTKG
                     FLPFAPEADFWVGKHGAPKIEIQMLDWKTQRTDAAAGVGLENWKVGPGKIDIALVRED
                     VDDYDRSLQNKQQINTNTIDLRYKDIPLWDKATLMVSGRYVTANESASEKDNQDNNGY
                     YDWKDTWMFGTSLTQKFDKGGFNEFSFLVANNSIASNFGRYAGASPFTTFNGRYYGDH
                     TGGTAVRLTSQGEAYIGDHFIVANAIVYSFGNDIYSYETGAHSDFESIRAVVRPAYIW
                     DQYNQTGVELGYFTQQNKDANSNKFNESGYKTTLFHTFKVNTSMLTSRPEIRFYATYI
                     KALENELDGFTFEDNKDDQFAVGAQAEIWW"
     gene            2805..>2992
                     /locus_tag="JNC24_004102"
     CDS             2805..>2992
                     /locus_tag="JNC24_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9160708.1"
                     /translation="MNIKIAALTLAIASGISAQWAIAADMPASPAPTIPVKQYVTQVN
                     ADNSVTFRYFAPGAKNVS"
BASE COUNT          838 a          632 c          744 g          778 t
ORIGIN      
        1 atcggctgtt tgatgaaatg gcgcaggcgg ggatcaggcc gctggtaacg ttatcccatt
       61 acgaaatgcc atatgggctg gtgaaaaact acggcggttg ggctaatcgg gcggtcatcg
      121 atcacttcga gcattacgcc cgcacggtct ttacccgcta ccaacataaa gtagcgttat
      181 ggctgacgtt taatgaaatc aatatgtcgt tacacgcgcc attcacgggc gtggggctgg
      241 cagaagagag tggcgaggcg gaagtttatc aggcgatcca tcatcaactg gttgccagtg
      301 cgcgggcagt taaagcctgt catagcctga tccccgaagc gaaaatcggc aatatgcttc
      361 tcggtgggct ggtttacccc ctcacctgcc agccacagga tatgttgcag gccatggaag
      421 agaaccggcg ctggatgttc tttggtgatg ttcaggcgcg tggccagtat cccggctata
      481 tgcagcgttt cttccgcgac cacaatatca ccattgagat gactgaaagt gacgcagaag
      541 atttaaaaca tactgtagat ttcatctctt ttagttatta catgactggt tgtgtttccc
      601 acgacgaaag cattaataaa aatgcgcagg gcaacatact gaatatgatc cccaatccgc
      661 atctgaaaag ttcagagtgg gggtggcaaa ttgatccggt tggattacgg gttctgttaa
      721 atacgctttg ggatcgttat caaaaaccgt tatttattgt cgagaacgga ttaggcgcaa
      781 aagacagcgt tgaagcggat ggttcgatac aggacgatta tcgaattgcc tatttaaacg
      841 atcacctggt acaggtaaat gaagcgattg ccgatggtgt ggatattatg gagtacacca
      901 gttgggggcc aattgattta gtcagtgcat ctcattcaca aatgtctaag cgctacggct
      961 ttatttatgt ggatcgtgat gataatggcg aaggaagcct cacaagaacg cgtaagaaaa
     1021 gcttcggatg gtatgcagaa gtgatcaaaa cgcgggggct gtcattaaaa aaataaccaa
     1081 taaagcacct taattatcgt cgcattcaga acagtctgga tgcgatacgt taattctttc
     1141 tttgcaccat aaagggatat tatgtttaga cgaaatctta ttacctctgc catcttatta
     1201 atggcaccgt tagccttttc tgcacaatca ttggctgaat cattaacggt ggaacaacgc
     1261 cttgagttat tagaaaaagc gttaagagaa acgcaaagcg aactcaaaaa gtataaagat
     1321 gaagagaaga aaaaatatac gccagcgacg gtgaatcgta gcgtaagtac gaatgatcaa
     1381 gggtatgccg ccaatccgtt cccgaccagt agtgccgcaa aacctgatgc tgtactggtc
     1441 aaaaatgaag agaaaaatgc cagtgagaca ggttcgattt attcttccat gactctgaaa
     1501 gatttcagta agtttgtgaa agatgaaatt ggctttagtt acaacggcta ttaccgttct
     1561 ggttggggga ccgcctctca tggttcacct aaatcatggg cgattggttc tctgggccgc
     1621 tttggtaacg aatactccgg ctggtttgat ttgcagttaa aacaacgtgt ctacaacgaa
     1681 aacggcaaac gggttgatgc cgttgtgatg atggatggta acgttggtca gcagtactct
     1741 accggctggt ttggcgataa cgccggtggc gagaacttta tgcagttctc cgatatgtac
     1801 gttaccacca aaggtttcct gccctttgcg ccagaggctg atttctgggt gggtaaacac
     1861 ggtgcgccga aaattgaaat ccagatgctg gactggaaaa cgcagcgtac tgatgccgca
     1921 gcgggtgtag gtctggaaaa ctggaaagtc ggtccgggta aaattgatat cgcgctggtt
     1981 cgcgaagatg tcgatgatta cgatcgcagc ctgcaaaaca aacagcagat taataccaat
     2041 actattgatt tacgctataa agatatcccg ttatgggata aagcgacctt aatggtgagt
     2101 ggtcgttatg tcacggcaaa cgaaagcgca tcggaaaaag ataatcagga taataacggg
     2161 tattatgact ggaaagatac ctggatgttc ggcacatctt taacgcagaa atttgataaa
     2221 ggtggcttca acgaattctc cttcctggtt gcgaataact ctatcgccag taactttggc
     2281 cgttatgctg gcgcaagtcc atttaccacc tttaatggtc gttattatgg tgatcacacc
     2341 ggcggaacag cagttcgtct gacctcgcag ggcgaagcct atatcggcga tcattttatt
     2401 gtggctaacg cgattgttta ctccttcggt aacgatattt atagctacga aacaggcgca
     2461 cactctgatt tcgaatctat tcgtgcggtt gttcgcccgg cctatatttg ggaccaatat
     2521 aaccagacag gtgttgaact gggctatttc acccagcaaa acaaagatgc gaatagtaat
     2581 aaatttaatg agtctggtta taaaaccacg ctcttccata cctttaaagt caataccagt
     2641 atgttgacct cgcgtccgga aattcgtttc tacgccacgt atatcaaagc tctggaaaac
     2701 gaactggatg gcttcacctt tgaagacaat aaagacgacc agtttgctgt cggtgcgcag
     2761 gctgaaatct ggtggtaatg gaacttaatg aaaggaatat tacgatgaat ataaaaattg
     2821 ctgctttaac gctggctatt gccagcggta tttctgctca gtgggccata gcagcggata
     2881 tgccagccag cccggcaccc actattccgg ttaaacagta tgtgactcag gtcaatgccg
     2941 ataacagcgt gacctttcgc tactttgccc ctggggcaaa aaacgtctcc tt
//
LOCUS       DADMKF010000277         2966 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.282, whole genome shotgun sequence.
ACCESSION   DADMKF010000277 DADMKF010000000
VERSION     DADMKF010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2966
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.282"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            <1..216
                     /locus_tag="JNC24_004103"
     CDS             <1..216
                     /locus_tag="JNC24_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HAY9160709.1"
                     /translation="DGTLYGATILGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLT
                     HILSEHFENVKTRVQGTVVMFSASGKK"
     gene            complement(271..369)
                     /locus_tag="JNC24_004104"
                     /pseudo
     CDS             complement(271..369)
                     /locus_tag="JNC24_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518755.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            complement(415..597)
                     /locus_tag="JNC24_004105"
     CDS             complement(415..597)
                     /locus_tag="JNC24_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAY9160710.1"
                     /translation="MLIGYVRVSTNDQNTDLQRNALVCAGYEQIFEDKLSGTRTGRPG
                     LKRALKRLQKGDALVV"
     gene            696..1586
                     /locus_tag="JNC24_004106"
                     /pseudo
     CDS             696..1586
                     /locus_tag="JNC24_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540327.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
     gene            1589..2122
                     /locus_tag="JNC24_004107"
     CDS             1589..2122
                     /locus_tag="JNC24_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000972171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HAY9160711.1"
                     /translation="MQHLKNIVAGNPKTVEQYQLTKNFDVIWLWSEDGKNWHEELKNF
                     QEDTIKLAYTVEGIIIAIDKDVSAINPEGLSVVELPDITANRRADISGNWMFKDGAVI
                     KRTYTEEEQRQQAENEKQSLLQLVRDKTQLWDSQLQLGIISDENKQKLTEWMLYAQKV
                     ESTDTSSLPVTFPEQPE"
     gene            complement(2151..2678)
                     /locus_tag="JNC24_004108"
     CDS             complement(2151..2678)
                     /locus_tag="JNC24_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001762409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HAY9160712.1"
                     /translation="MMHLKNIKAGNPKTPEQYQLTKKAGVVWLFCEDGKNWYEEQKNF
                     QPDTIKIAYDENNIIVAVSKDVSTINPEGLSIVEVPDITANRHADNNGKWMFLDGKVV
                     KREYTEQELQQQAESQKAALLSEAESVIQPLERAVRLNMATDEERTRLEAWERYSVLV
                     SRVDTANPEWPQKPE"
     gene            complement(2680..>2966)
                     /locus_tag="JNC24_004109"
     CDS             complement(2680..>2966)
                     /locus_tag="JNC24_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000145463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY9160713.1"
                     /translation="ALGSGAQVASSGDIYGSVWENNWLSTWLHNHVVRDIRLGSIEYK
                     NVWRDYGFGDASGYVLTAAINSNADDIVDTVARRPIQKLIGGIWYNVGSV"
BASE COUNT          838 a          661 c          649 g          818 t
ORIGIN      
        1 gatggaactc tatacggagc cacaattctt ggcgatggag ttgtgcacaa tagcttcggt
       61 caaaaactga tgcgcattta caatcagaaa ggcatctttt caaacacaaa agattccgaa
      121 gaaggcttaa cacatatact ctcagagcat ttcgagaatg ttaaaaccag ggttcaaggt
      181 actgtagtaa tgttttccgc ttcagggaaa aaatagcatc caaccgcagc acgttcttgc
      241 ttaagacgtg ctgcggcata atcccaatga ttactccctg acagggttcg taggccactc
      301 aatatcaggt gcagttgatg tatcaacacg gttcagcaac acccgatact ttttctaggc
      361 ttccaccacc agcacgacaa gatgccgcat acagtgcccc agtcagtcca gttttcagac
      421 aaccagtgcg tcaccttttt gaaggcgctt taaagcacgt tttaatccag gtcggcctgt
      481 ccttgttccg cttaatttat cttcaaatat ttgttcatat cctgcacaaa caagagcgtt
      541 tcgttgcagg tctgtattct ggtcatttgt tgataccctt acatagccaa tcagcacgct
      601 gaatctcccg tccaaaagca caaatcatgc catgcaggcc agaaaccgcc attatctaaa
      661 acctcggttt acgagaactc ggcacaagcg gggagaaaat accgttactc agtacagcga
      721 atacctggac taatcgacaa acattcagcg gtggcctttc tggtgaacta tccggcaatg
      781 cttctacagc tgcaaaatta aaaactgcca ggaaaataag caatgtggct tttgatggtt
      841 cctccgatat cacattaaaa gcaagtcatg ttggtgcgtt tgccttaggg aaaacaggaa
      901 gcaccgttgc gaatgataaa gcagttggat ggaactggag tagcggagcc tataacgcaa
      961 ctattagtgg tgcatcaacg ttaattattc atttttatat gggagaagga agttgtcctg
     1021 cagctcagtt tcggattaat tataaaaatg gcggtatttt ttatcgttca gcccgtgatg
     1081 gttatggttt tgaagccgac tggtccgaat tttacaccac cagaaaacct tcagcaggag
     1141 atgttggtgc actgccgtta tctggtggtc aactgaatgg tgcactgggt atcggaacat
     1201 ccagtgctct tggcggtaat tcgattgtat tgggtgataa tgacacgggc tttaaacaaa
     1261 aaggcgatgg taatctggat gtttatgcta ataacgtcca tgttatgcgc tttgtctccg
     1321 gaagcattca aagtaataaa accataaata ttacgggacg tgttaatccc tcggattacg
     1381 gtaactttga ttcccgctat gtgagagatg tcagacttgg cacacgagtt gtttagacca
     1441 tgaagaaagg cgtgatgtat gaaaaatcag gccatgtaat cacggggctt ggtattatcg
     1501 gtgaagtcga tggtgatgat ccggcagtat tcagaccaat acaaaagtta attaacggaa
     1561 catggtataa cgtatcgcag gtataatcat gcagcattta aaaaacattg tcgcaggtaa
     1621 tccaaaaacc gttgaacaat accagctaac aaagaatttt gatgttatct ggttatggtc
     1681 cgaagatgga aaaaattggc atgaggaatt aaaaaacttt caggaggata caataaaact
     1741 ggcttacaca gttgagggaa taattattgc tatagacaag gatgtatcag caattaatcc
     1801 agaaggttta agtgtcgttg agttgcctga tattacagca aatcgccggg ctgatatttc
     1861 gggcaactgg atgttcaaag atggtgcggt gataaagcga acttataccg aggaagagca
     1921 aaggcaacaa gcggaaaatg aaaagcagag cctgctgcag ctcgtcaggg ataaaaccca
     1981 gctgtgggac tctcagctac agctgggcat catttccgat gagaataaac aaaaattaac
     2041 cgagtggatg ctctatgcgc agaaggttga atccacagac acctccagcc tgccagtaac
     2101 gtttcccgaa caaccagaat gagagaaggc ccgctatcgg gccttaattt ttattcaggc
     2161 ttttgtggcc attcaggatt tgccgtatcc acacggctga ccagaacact gtagcgttcc
     2221 catgcttcca gtcgtgtgcg ttcctcgtct gttgccatat tcagcctgac agctcgttcc
     2281 agcggctgga tgactgattc agcttcggaa agcaatgcgg ccttttgtga ttcggcctgt
     2341 tgttgcagtt cctgttctgt atattccctc tttactactt taccatccag aaacatccat
     2401 tttccgttat tgtctgcgtg acgatttgcc gtaatatcag gaacttcaac tatgcttagc
     2461 ccttcaggat taatagttga aacgtcttta ctgacagcaa cgatgatgtt gttctcatcg
     2521 taagcaattt ttatggtgtc tggctgaaaa ttcttttgtt cctcatacca gtttttgcca
     2581 tcttcgcaaa agagccatac cacgcccgct tttttagtta gctggtattg ttccggtgtt
     2641 ttcggattcc cggccttaat atttttcagg tgcatcatct taaacactcc ccacgttgta
     2701 ccatattccc ccaatcaatt tctgaattgg ccttctggca acagtgtcga caatatcatc
     2761 cgcattgctg ttaattgcgg ctgttaaaac ataacctgac gcatcgccaa agccgtagtc
     2821 gcgccatacg tttttatatt caatgctgcc aagacgaata tcccgaacga catgattatg
     2881 cagccatgtg cttaaccagt tgttttccca cactgaacca taaatatcgc cactagacgc
     2941 aacttgcgca ccacttccca gcgcaa
//
LOCUS       DADMKF010000278         2944 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.283, whole genome shotgun sequence.
ACCESSION   DADMKF010000278 DADMKF010000000
VERSION     DADMKF010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2944
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.283"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            <1..312
                     /locus_tag="JNC24_004110"
     CDS             <1..312
                     /locus_tag="JNC24_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9160714.1"
                     /translation="ALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMFTEMFGASYRYS
                     GAGVGYQVASVVGGGFTPFIAAALITYFAGNWHSVAIYLLAGCLISAMTALLMKDSQR
                     A"
     gene            414..1868
                     /gene="amn"
                     /locus_tag="JNC24_004111"
     CDS             414..1868
                     /gene="amn"
                     /locus_tag="JNC24_004111"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HAY9160715.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGNYITSGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSAGLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWNLITADAQGITLV
                     NIGVGPSNAKTICDHLAVLRPDVWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
     gene            2199..2915
                     /locus_tag="JNC24_004112"
     CDS             2199..2915
                     /locus_tag="JNC24_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAY9160716.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNAGVIVFKGSDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPTDLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYHNVANL"
BASE COUNT          799 a          655 c          662 g          828 t
ORIGIN      
        1 gcgcttgaag cacaatctat tttctggata gttttcttct ccataatgct ggcaaacatt
       61 gcgcatgaca tggtggtgtg tgtgcaacaa ccgatgttta ccgaaatgtt tggtgccagt
      121 tatcgctata gtggcgctgg agtcggttat caggttgcca gtgtggttgg cggtggattt
      181 acacctttta ttgccgctgc actcatcact tactttgccg ggaactggca tagcgtcgcc
      241 atttatttgc tggctggatg cctgatttcc gcaatgaccg ctttgttgat gaaagacagt
      301 caacgcgctt gatagcctgg cgaagatcat ccgatcttcg ccttacactt ttgtttcaca
      361 tttctgtgac atactatcgg atgtgcggta attgtatgga acaggagaca cacatgaata
      421 ataagggctc cggtctgacc ccagctcagg cactggataa actcgacgcg ctgtatgagc
      481 aatctgtagt cgcattacgc aacgccattg gcaactatat tacaagtggc gaattacctg
      541 atgaaaacgc ccgcaaacaa ggtctttttg tctatccatc actgaccgta acctgggacg
      601 gtagcacaac caatcccccc aaaacgcgcg catttggtcg ttttactcac gcaggcagct
      661 acaccaccac gattactcgc cctactctct ttcgttcgta tcttaatgaa caacttacgt
      721 tgctgtatca ggattatggt gcgcatatct ctgtgcaacc ctcgcagcat gaaatccctt
      781 atccttatgt catcgatggc tctgaattga cacttgatcg ctcaatgagc gctgggttaa
      841 ctcgctactt cccgacaaca gaactggcgc aaattggcga tgaaactgca gacggcattt
      901 atcatccaac ggaattctcc ccgctatcgc attttgatgc gcgccgcgtc gatttttccc
      961 tcgcacggtt gcgccattat accggtacgc cagttgaaca ttttcagccg ttcgtcttgt
     1021 ttaccaacta cacacgttat gtggatgaat tcgttcgttg gggatgcagc cagatcctcg
     1081 atcctgatag tccttacatt gccctttctt gtgctggcgg gaactggatc accgccgaaa
     1141 ccgaagcgcc agaagaagcc atttccgacc ttgcatggaa aaaacaccag atgccagcat
     1201 ggaatttaat taccgccgat gctcagggta ttactctggt gaatattggc gtgggaccat
     1261 caaatgctaa aaccatctgc gatcatctgg cagtgctacg cccggatgtc tggttgatga
     1321 ttggtcactg tggcggatta cgtgaaagtc aggccattgg cgattatgta cttgcacacg
     1381 cttatttacg cgatgaccac gttcttgatg cggttctgcc gcccgatatt cctattccga
     1441 gcattgctga agtgcaacgt gcgctttatg acgccaccaa gctggtgagc ggcaggcccg
     1501 gtgaggaagt caaacagcgg ctacgtactg gtactgtggt aaccacagat gacaggaact
     1561 gggaattacg ttactcagct tctgcacttc gttttaactt aagccgggcc gtagcaattg
     1621 atatggaaag tgcaaccatt gccgcgcaag gatatcgttt ccgcgtgcca tacgggacac
     1681 tactgtgtgt ttcagataaa ccattacatg gtgagattaa acttcccggt caggctaacc
     1741 gtttttatga aggcgctatt tccgaacatc tgcaaattgg cattcgggcg atcgatttgc
     1801 tgcgcgcaga aggcgaccga ctgcattcgc gtaaattacg aacctttaat gagccgccgt
     1861 tccgataata agttctgaac cttcttatca gaaatagatg tgaaggagta agtaagacac
     1921 ctggcaaata gcctgcaata atcgtgggct gtttgcttcc ttgggcggat acgagtttta
     1981 ttatcgtctt aatgatttcc acatattaaa agcaagtatg ctttcaaaac acaattataa
     2041 aaaatcccgc caacaatata agtttttata aaattatggc tttagaatat ttttatttct
     2101 aatagacgag atttttcctg ttatgatata atatgccgaa ttaacacatg ttaacgattt
     2161 accagtaatg taaataaatt ttcgaggaga tcattccagt gggacgtaaa tgggccaata
     2221 ttgttgctaa aaaaacggct aaagacggtg caacgtctaa aatttatgca aaattcggtg
     2281 tagaaatcta tgctgctgct aaacaaggtg aacccgatcc agaattaaac acatctttaa
     2341 aattcgttat tgaacgtgca aagcaggcac aagttccaaa gcacgttatt gataaagcaa
     2401 ttgataaagc caaaggcggc ggagatgaaa cgttcgtgca gggacgttat gaaggctttg
     2461 gtcctaatgg ctcaatgatt atcgccgaga cattgacttc aaatgttaac cgtacgattg
     2521 ctaacgttcg cacaattttc aataaaaaag gcggcaatat cggagcggca ggttctgtca
     2581 gctatatgtt tgacaatgct ggtgtgattg tatttaaagg atcagaccct gaccatattt
     2641 ttgaaatttt acttgaagct gaagttgatg ttcgtgatgt gactgaagaa gaaggtaaca
     2701 ttgttattta taccgaacct actgaccttc ataaaggaat cgcggctcta aaagcagctg
     2761 gaatcactga gttctcaaca acagaattag aaatgattgc tcaatctgaa gttgagcttt
     2821 ccccagaaga tttagaaatc tttgaagggc ttgttgatgc ccttgaagat gacgacgatg
     2881 tacaaaaagt ttatcataac gtcgcaaatc tctaattatc ttttaaagaa atctgtcttt
     2941 acgg
//
LOCUS       DADMKF010000279         2939 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.284, whole genome shotgun sequence.
ACCESSION   DADMKF010000279 DADMKF010000000
VERSION     DADMKF010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2939
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.284"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            37..264
                     /locus_tag="JNC24_004113"
     CDS             37..264
                     /locus_tag="JNC24_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HAY9160717.1"
                     /translation="MRLIVVSIMVTLLSGCGSIISRTIPGQGHGNQYYPGVQWDVRDS
                     AWRYVTILDLPFSLVFDTLLLPIDTHHGPYE"
     gene            complement(265..639)
                     /locus_tag="JNC24_004114"
     CDS             complement(265..639)
                     /locus_tag="JNC24_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secY/secA suppressor protein"
                     /protein_id="HAY9160718.1"
                     /translation="MTMYATLEEAIDAAREEFLADNPGIDAEDANVQQFNAQKYVLQD
                     GDIMWQVEFFADEGEEGECLPMLSGEAAQSVFDGDYDEIEIRQEWQEENTLHEWDEGE
                     FQLEPPLDTEEGHAAADEWDER"
     gene            complement(722..1768)
                     /gene="mdtG"
                     /locus_tag="JNC24_004115"
                     /pseudo
     CDS             complement(722..1768)
                     /gene="mdtG"
                     /locus_tag="JNC24_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtG"
     gene            complement(2099..2674)
                     /locus_tag="JNC24_004116"
     CDS             complement(2099..2674)
                     /locus_tag="JNC24_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAY9160719.1"
                     /translation="MSRTFAYCRVSVETSTTENQIQAIKKAGYDIPKSRVIEEVVSGS
                     VSAMDRDGFANLVNHKLEEGDTLVVLKLDRLGRDNIDLQNTVTLLNDKGVKLICLDLP
                     VKDLSSSEGKLVLQLFSTFAEFERNRIRERTKQGLERARVQGKVLGRPIASDTTRAVL
                     EYKERGLSQSQVAKVLNLSVRTVSRHWTKTL"
BASE COUNT          762 a          696 c          688 g          793 t
ORIGIN      
        1 cgttatgccg gtattttttt acggaagagg tatcaaatgc gtttaattgt ggtgagcatc
       61 atggtgacct tgctgagtgg ctgtggcagc atcattagcc gcactatacc ggggcagggg
      121 cacggcaacc aatattatcc cggtgtgcaa tgggatgtgc gtgactccgc ttggcgttat
      181 gtcacaatcc ttgatctgcc attctccctg gtttttgata ctttactgct gccgatcgac
      241 actcatcatg gcccgtatga gtgattaacg ttcatcccac tcatcagctg cagcgtgtcc
      301 ttcctcggta tccagcggtg gctcaagctg aaattccccc tcgtcccatt catgtaatgt
      361 attctcttcc tgccactcct ggcggatctc tatctcatca tagtcgccat caaaaacact
      421 ttgcgcggct tcaccgctaa gcataggtaa acattcacct tcttcccctt cgtcggcaaa
      481 aaactcaact tgccacatga tgtcgccgtc ctgcaaaacg tatttttggg cattgaactg
      541 ttgcacattc gcatcttcgg cgtcgatgcc ggggttgtct gcaagaaatt cttcgcgtgc
      601 agcgtcaatg gcttcttcaa gcgttgcgta catggtcatc acgattctcc ctttgagttg
      661 atgaggtttc agggaaaagg atagctgatt ctccgctttt gcaagtatga aaggcgaaaa
      721 atcagttcga tacctggggt attcgacgac gacgtagact gttccatgaa tagactgcgt
      781 tgaataacac tacgccagcg gtgacgagaa ataccgctct gaaaccgtag ttcgctgaaa
      841 tcgctgctcc catcaatggt ccggtaacgt tgccaatatc acgaaacgat tggttatagc
      901 tgaagatacg tccggcgatt tggttgctcg agttataaac taacagtgtc tgtacggcgg
      961 ggagtagtgc accatcggcg gcaccgagca aaaaacgtaa aatcccaagt tgcaatggcg
     1021 tctgaacgta agacattggg atcaacagca gtacagaaaa gatcagcgcc gtaatcagga
     1081 tcttttcggg tccgattcga tcgccaagtt tgccgagtcg tggtgcactt agcagagccg
     1141 ccacgcctgg caccgaggcg atcatgccac tgataaaggc gacgttactg acgttacccg
     1201 ccagttcgcg gacatacagc gtcagaatgg gggcaattga gcccgtcgcc acctggatga
     1261 ttaacgtagt gacaaacagg ctgagtacca gtttcgggtt tttaagtgat gtcaccactt
     1321 cccgcatgtg cagcatctct tttttgctga tcggctggaa tttttctctt atgcaaaaca
     1381 gggtgacgaa aaagcagagt atgagcacac tggcggtaat aaagaatacc ggacgtaagc
     1441 cgtagctatc ggcgagcagg ccgccagcca ttgggccgag caacgcacca ctaacgccgc
     1501 ctgtggagag cgtacccagc gcccagccgc ttttattacg cggtacttgt gtggcgataa
     1561 gagcattagc gttggggaca aatccgccaa gtaacccaag aagcgcccgc aggatcaaaa
     1621 actgccagat attttgtgcc agccccatca acaccatcac gatgcccatg ccgagggcag
     1681 agcgtaatag catgagtttt cggcctttac ggtcggcgag tccaccccaa aacggtgagg
     1741 cgatggccga aaataaaaat gtaatgctca tatatgctat cttaatgcac tacctgataa
     1801 gtgagcctat acaggttatt gctgtatgtt tatacagtat tattggtaga agactgtgta
     1861 gatttggtat agagatgaat agatactatt aaggtaactt attgaaatca ataaagattc
     1921 ttgtgcagtc tgaatagata acttagcgtt atgtctagtc gtttccctct ccattcctaa
     1981 aaagtgctgt atactggcct atacaacgct ataaagacct ctacatagcg ccttgatagc
     2041 tatgtcacag gtacaaaaaa gccccctata aaggaggctg tgtagtctat taagggtctt
     2101 acagggtctt ggtccagtgt ctgctaacag tccttacaga gaggttaaga accttagcta
     2161 cctgagactg agatagtccc ctttccttgt actctaagac cgctctagtt gtgtcactcg
     2221 ctataggtct tccaagtacc ttaccttgaa ctctagcacg ctctagaccc tgtttagtgc
     2281 gctctctgat acggtttctt tcaaactctg caaaggtact aaatagctgt agaaccaact
     2341 taccctctga gcttgaaagg tctttaacgg gtaaatctaa acagatgagc ttcacaccct
     2401 tatcattaag aagggtcact gtattctgta ggtcgatgtt atcccttccc agacggtcaa
     2461 gtttaagaac aacaagggtg tcaccttctt ctagcttatg gttgaccaga ttcgcaaatc
     2521 cgtccctatc catagctgac actgaaccag agacaacctc ctctataact cgtgacttag
     2581 gaatatcata gccagccttc ttaatggctt gaatctggtt ctctgtggtg gaagtctcta
     2641 ctgatacacg gcagtaagca aaggttcgtg acatagatat ccctctgggt agtcattaaa
     2701 cggttagttt gtcttgtcta tagacattaa agcaaatggt agatttgttg tctatagcta
     2761 tagtgactct tctatgtaga cattaaacgg acgtttcttg tcgtgggtca catagatact
     2821 tttagggctt ctgtcaagtg atattcgtag attttgcaag ggggaggtgt aaggctgaaa
     2881 cagggctact aagaacacat acatctaagg ctggaaagta cctgagcaga tattcttca
//
LOCUS       DADMKF010000280         2879 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.285, whole genome shotgun sequence.
ACCESSION   DADMKF010000280 DADMKF010000000
VERSION     DADMKF010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2879
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.285"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(<1..953)
                     /gene="curA"
                     /locus_tag="JNC24_004117"
     CDS             complement(<1..953)
                     /gene="curA"
                     /locus_tag="JNC24_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HAY9160720.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQPSDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATG
                     PVGATVGQIGKLKGCRVVGVAGGAEKCRHAIEVLGFDVCLDHHADDFAEQLVKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQKEMGQWVKEDKIHYHEDITDG"
     gene            1134..1652
                     /gene="mddA"
                     /locus_tag="JNC24_004118"
     CDS             1134..1652
                     /gene="mddA"
                     /locus_tag="JNC24_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HAY9160721.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHHSLGFVVTAQMPQVGTKFGRWLDLTFMQL
                     QLDERTEPDAIG"
     gene            1649..2098
                     /locus_tag="JNC24_004119"
     CDS             1649..2098
                     /locus_tag="JNC24_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HAY9160722.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMVRITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLMLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(2099..2332)
                     /locus_tag="JNC24_004120"
     CDS             complement(2099..2332)
                     /locus_tag="JNC24_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcY family protein"
                     /protein_id="HAY9160723.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(2418..2591)
                     /locus_tag="JNC24_004121"
     CDS             complement(2418..2591)
                     /locus_tag="JNC24_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GhoT/OrtT family toxin"
                     /protein_id="HAY9160724.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            2786..>2879
                     /gene="yncL"
                     /locus_tag="JNC24_004122"
     CDS             2786..>2879
                     /gene="yncL"
                     /locus_tag="JNC24_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HAY9160725.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
BASE COUNT          691 a          738 c          692 g          758 t
ORIGIN      
        1 aaaccatcag taatatcttc gtggtagtgg attttatcct ctttcaccca ttgccccatc
       61 tccttctgaa actcatggat gcggtgacca taatcctgag cgataataaa cccttgcaag
      121 cgaatacgtt ttttcagcac cgttgccatc aacaggggta aacgatccgg accgggtggt
      181 agctctgtag cgttatagct gctcactaag ccacaaacgg gaatgcgcgc agatgtatta
      241 agtaatggta gcactgcatc aaatacctta ccgcccacgt tttcataata gatatcaata
      301 ccttttgggc acgctttcac cagttgttcg gcaaaatcat ccgcgtggtg atcaagacaa
      361 acatcgaagc ccagcacctc gatagcatgg cggcattttt ccgcgccacc ggcaaccccc
      421 accaccctgc aacctttaag tttgccgatt tgccccaccg ttgctcccac aggacctgtc
      481 gccgcagcta ccaccaacgt ttcgccctct ttaggctgac cgatatccag cagccccata
      541 taagcggtaa agcctggcat ccccagcaca cccagcgacc acgatggatt ttgcggatga
      601 tcgccaagtt tcaccagatc atcaccactg gatatgtcat agtcctgcca tccactgtag
      661 cccagcaccc agtcgctagg ctgataatca ggatgattcg actccacaac acggctcacc
      721 gtgccgccga ccatcacgcc gccaatatca acaggcggtg aataagatgg ctcatcgctc
      781 atacgtccac gcatatacgg gtccagggac aaataaactg tgcgcagtaa cacctgtcct
      841 tcacccggtg tggcgacatc atcttcttca agacggaaat tctccggaac cggtgcgcca
      901 tgtggacgcg aggccagaac ccaacgtcga ttacgctgct tttgttgccc cataatgctc
      961 tcctgtgctt ttggcatgaa tccatcaata gtagcaggct cacgggattg gttttgtttg
     1021 cagccgtcac atcccctctg caacacgcaa taatttcatc gaggaatccg ttgcttcggg
     1081 ctgttttcga tagtattgat aacatcatca tcattatttt atcgaggcca gacatgtcca
     1141 tccgttttgc ccgcaaagcc gattgtgctg ccattgcgga aatttataac cacgccgtgt
     1201 tgtatacggc ggctatctgg aatgaccaaa cggttgatgc agataaccgc attgcctggt
     1261 ttgaagcgcg gaccatagca ggttatccgg tgctggtaag tgaagaagat ggcgtagtga
     1321 cgggatatgc ctcgtttggc gactggcgta gtttcgatgg ttttcgccat accgtggaac
     1381 attcggttta tgtccatccc gatcatcagg gcaaaggtct ggggcgtaaa ttgttaagcc
     1441 gattgattga tgaagcgcgg gattgcggga agcatgtcat ggtcgccggg atcgaatcgc
     1501 aaaatcaggc ctcgctgcat ctgcaccatt cgctgggatt tgtcgtcacc gcacaaatgc
     1561 cgcaggtggg cactaaattt gggcgctggc tggatctgac atttatgcag ttgcaactcg
     1621 acgaacgcac tgaaccggac gcgattggat gaatcagtcg ctcacccttg cttttttgat
     1681 tgccgccgga attggtctgg tagtccagaa cacgctgatg gtgcgcatca cccagacctc
     1741 ctctaccatt ctcatcgcca tgttgctgaa ctcactggtg gggattgtgc tctttgtttc
     1801 tattttatgg tttaaacaag gcatggcggg gtttggcgaa ctggtgtcca gcgtgcgctg
     1861 gtggacactt atacccggct tgctgggatc gttttttgtc ttcgccagta tcagcggata
     1921 tcagaatgtg ggagcggcga caactattgc agtactggtc gccagccagc taattggcgg
     1981 tctgatgctg gatatcttcc gcagtcatgg cgtaccacta cgggcgttgt tcggcccaat
     2041 ttgtggcgca attttacttg tggttggcgc ctggttggta gccagacgct cattttaatc
     2101 agaggatggt gccgcctttg gttaactgtt cgtggcgcgc ttccatatct tctttatgct
     2161 tgcgaccgtg atgagcaata gcggtgcgca aacgctgctg ctgggtgtag cgatcttccc
     2221 gacttaactc cgcgtcatcg ctcagggcga ttaataattc gttcatatgg gcaatgacgc
     2281 tctcttcaat cgcggcatcc acgcgcgcga tgacttcatc cagatgcgac atactttctc
     2341 cttaaccact tgtgaaattt atcgattcat ccaaatgata taagtctgcg tggtgccgtc
     2401 aagccagtga gtaagattta aaacagtgaa aacaacagcg ccaccgggaa actcatcggc
     2461 catgttgccc ccaccagaaa ggcgcttaag aaacggatgc gtttcttatc gtgagaaaga
     2521 aaccaggtga taagaaatgc gattgctgcc ataaccgcat aaaaaacaag catatgttga
     2581 tagagagaca tgtacgggcg tccagccaga tgaataaaac gcgcgcaaat atgcgtcatt
     2641 ttttgacgtg catcaagttt tttcaccagt ttgagaaaaa atcatcaaac agcaacttgc
     2701 ccttatccat cttcagaatg agtattattg cggattttct taacccgtac tatacacata
     2761 ggggcaatga taaaaggtgg caaaaatgaa tgtttccagt agaactgtag tactgataaa
     2821 tgtctttgct gctgttggtt tgtttactct tatctctatg agatttggct ggtttattt
//
LOCUS       DADMKF010000281         2814 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.286, whole genome shotgun sequence.
ACCESSION   DADMKF010000281 DADMKF010000000
VERSION     DADMKF010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2814
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.286"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            1..989
                     /locus_tag="JNC24_004123"
                     /pseudo
     CDS             1..989
                     /locus_tag="JNC24_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480735.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
     gene            1005..1754
                     /locus_tag="JNC24_004124"
     CDS             1005..1754
                     /locus_tag="JNC24_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HAY9160726.1"
                     /translation="MRYLNTKNLIAAGVLLACMSSIAWGAIIPDRTRIIMNESDKGEA
                     LKLTNQSKNLPYLAQTWIEDTKGNKSRDFIVTVPPMVRLNPSEQIQIRMISQEKIAQL
                     PNDRETLFYFNVREIPPKTDKKNVMQVTMQHALKLFWRPKAIELEDDGVMSYEKVEII
                     RRNDGSIRFNNKMPYHVTLGYIGTNGITMLPQTQSLMVTPYSHADTQFKNVPSAFQVG
                     YINDFGGLSFYEINCPTVNNSCNVSVAKRDK"
     gene            1756..>2814
                     /locus_tag="JNC24_004125"
     CDS             1756..>2814
                     /locus_tag="JNC24_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9160727.1"
                     /translation="MRYLLIVIIFIIGFSALPVWAMDCYAEHEGGNTVVIGYVPRISI
                     PSNGKKGDKIWQSSEYFMNVFCNNALPAPSPGEEYPSAWTNIMMFLPGGQDFYNQNSY
                     IFGVTYNGVDYDSTAPNAIAAPECIDIKGAGTFNNHYKNPAVCSGGPEPQLSVTFPAR
                     VQLYIKLAKNANRVNKNLVLPDEYIALEFKGMSGAGAIEVDKNLTFRIRGLNNIHVLD
                     CFVNVALEPADGVVDFGKINSRTIKNTSVSETFSVVMTKDPGAACTEQFNILGSFFTT
                     DILSDYSHLDMGNGLLLKIFHKDGTATEFNRFSQFASFSSSSAPSVTAPFRAELSANP
                     AETVVEGPFSKDVILKITY"
BASE COUNT          885 a          566 c          585 g          778 t
ORIGIN      
        1 aggttaaata catacgtaag tttggctcgt aatacttact gggacgccag cagtaatgtg
       61 aattattcat tatcacttag ccgcgatttt gatatcgggc cattaaaaaa cgtctccgct
      121 tctctaacat ttagccgaat aaactgggaa gaagacaacc aggatcaact gtacctaaat
      181 atttcgattc cctggggaac cagtagaaca ttgagctatg gtatgcaacg aaatcaggat
      241 aacaagattt cgcatactgc ttcgtggtat gactcttccg atcgaaataa ttcctggagc
      301 gtttctgctt caggcgacaa tgatgaattc aaagatatga aagcgtcact acgcgccagt
      361 tatcagcata ataccgagaa cggtcgtctc tatctctccg ggacatcaca gcgagacagt
      421 tattattctc tgaatgccag ttggaatggt tcattcacgg cgactcgtca cggagccgct
      481 ttccatgact atagtggtag tgctgactcg cgttttatga tcgacgcaga tggtgcagaa
      541 gatattccgt tgaacaataa acgcgcggta actaatcggt atggcatcgg agttattcca
      601 tcagtcagca gttacataac aacatcatta agcgttgata cccgaaatct tccagaaaat
      661 gtggatatcg aaaactcggt tatcaccacc accttaaccg aaggtgctat tggctacgcc
      721 aaacttgata ccccaagggc tatcaaatca tgggggttat tcgcctggca gatggtagtc
      781 acccaccact ggggattagc gtaaaagata aaaccagcca taaagaatta ggactggttg
      841 ctgatggcgg ctttgtatac ctcaacggca ttcaggatga cagcaaactt actttacgct
      901 ggggtgataa atcctgtttt attcaaccac ccaatagcag caacttaacc accggaacgg
      961 ttattttacc gtgtattagc caaaattaat tcaggatgaa actaatgcgc tacttaaaca
     1021 caaagaatct cattgcagca ggagtattac tcgcctgcat gagcagcatt gcctggggtg
     1081 ccatcatccc ggaccgtacc cgtatcataa tgaatgagtc agataaaggg gaagcattaa
     1141 aacttactaa tcaaagcaaa aatctaccct atctggcgca aacatggata gaagatacga
     1201 aaggcaataa atcacgtgac tttattgtca cggttccccc tatggtacgt ttaaatccca
     1261 gtgagcaaat tcaaataaga atgataagtc aggaaaaaat tgctcaactg cctaatgaca
     1321 gagaaacgct cttctatttt aacgtgcgag aaataccgcc aaaaacagat aaaaaaaatg
     1381 tgatgcaagt cactatgcag cacgcattga aactattctg gcgaccaaaa gctattgaat
     1441 tagaagatga tggggtaatg tcttacgaaa aagttgaaat catccgtaga aatgatggtt
     1501 cgatccgctt taacaataag atgccttatc acgttacgct gggttatatc ggaacaaacg
     1561 gaatcacaat gttgccacaa acgcaaagtt taatggtaac cccttatagc catgcagata
     1621 cgcagtttaa aaatgttccg tcagcttttc aggttggtta tatcaatgat tttggtggat
     1681 taagttttta tgaaataaac tgcccgacgg ttaataactc ttgtaatgtt tccgtagcca
     1741 aacgagataa ataatatgcg ctacttatta attgtaatta tatttattat aggttttagt
     1801 gcattacctg tatgggcaat ggactgctat gctgaacatg aagggggaaa taccgtagtc
     1861 attggttacg taccaagaat ttctatcccc agtaatggta aaaaaggtga taagatctgg
     1921 caaagcagtg agtattttat gaatgtcttc tgtaataatg cattacctgc cccatcacct
     1981 ggagaagaat acccatcagc ctggactaat ataatgatgt ttttaccagg cggtcaggac
     2041 ttttataatc agaactccta tatttttggc gtaacataca atggagttga ttatgattcg
     2101 actgccccta atgctatcgc agcaccagaa tgcattgata taaaaggagc gggtaccttt
     2161 aataatcact ataaaaaccc cgctgtctgt agtggaggtc ctgagcctca attatcagta
     2221 acctttccag ctcgcgtaca actctatatt aaattagcaa aaaatgccaa tagagttaat
     2281 aaaaacctgg tgttacctga tgaatatatc gctctagagt ttaaaggtat gagtggcgca
     2341 ggagctatag aagtagacaa aaatttgacc ttcaggattc gtggattaaa taatattcat
     2401 gtccttgact gctttgttaa tgtcgcactg gaaccagctg atggcgttgt tgactttggt
     2461 aaaataaatt cccgaacaat taaaaatacc agcgttagtg agacgtttag tgtagtcatg
     2521 acaaaagatc ctggtgcggc ctgtactgag caattcaata ttttagggag ctttttcact
     2581 acggatattt tgagtgatta tagccatctg gatatgggta atggactgct attgaagata
     2641 tttcataaag atggaacagc aacggaattt aaccgcttct cacaatttgc ttctttttca
     2701 tcgtctagtg caccttcggt caccgcacca ttcagggcag aacttagtgc gaacccggca
     2761 gagactgttg ttgagggacc gtttagtaaa gacgtaatcc tcaaaatcac ctat
//
LOCUS       DADMKF010000282         2788 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.287, whole genome shotgun sequence.
ACCESSION   DADMKF010000282 DADMKF010000000
VERSION     DADMKF010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2788
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.287"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            142..726
                     /locus_tag="JNC24_004126"
     CDS             142..726
                     /locus_tag="JNC24_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY9160728.1"
                     /translation="MSKKLGFALSGLMLAMVAGTASADMDGGQLNISGLVVDNTCETR
                     VDGGNKDGLILLQTATVGEIDAGVLNDTVGAKAKPFSITVDCSKANPNPGSTAKMTFG
                     SVFFGNSKGTLNNDMSINNPSDGVNIALHNIDGSTIKQVQINNPGDVYTKALDATTKS
                     AVYDFKASYVRAVADQTATAGYVKTNTAYTITYQ"
     gene            831..1571
                     /locus_tag="JNC24_004127"
     CDS             831..1571
                     /locus_tag="JNC24_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HAY9160729.1"
                     /translation="MFFNTKHTTALCFVTCMAFSSSSIADIVISGTRVIYKSDQKSVN
                     VRLENKGNNPLLVQSWLDTGDNNAEPGSITVPFTATPPVSRIDAKRGQTIKLMYTAST
                     SLPKDRESVFWFNVLEVPPKPDAEKVANQSLLQLAFRTRIKLFYRPDGLKGNPSEAPL
                     ALKWFWSGSEGKASLRVTNPTPYYVSFSSGDLEASGKRYPIDVKMIAPFSDEVMKVTG
                     LNGKASSAKVHFYAINDFGGAIEGNASL"
     gene            1606..2788
                     /gene="htrE"
                     /locus_tag="JNC24_004128"
                     /pseudo
     CDS             1606..2788
                     /gene="htrE"
                     /locus_tag="JNC24_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414681.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Yad fimbria usher protein HtrE"
BASE COUNT          798 a          584 c          596 g          810 t
ORIGIN      
        1 tcctaatgaa caatggttgt aaattttggt aacaaatatc attgctcatt aatatctagc
       61 atctgcttat aacatttgtg tcatgaatag tcattcttca aaccagtagt ctgactaatg
      121 gaattttatt caaggatttt tatgtctaaa aaattaggtt ttgccctgtc tggtcttatg
      181 ctggcaatgg ttgcaggtac tgcttctgct gatatggatg gtggtcagtt aaatatcagt
      241 ggtctggttg ttgataacac ctgtgaaacc cgcgttgacg gcggtaataa agatggtctg
      301 atcctgctgc aaactgcaac tgttggtgaa atcgatgctg gcgtgctgaa tgacaccgtt
      361 ggcgctaaag ccaaaccttt cagcatcacc gttgactgca gcaaagcaaa tccgaatcca
      421 ggtagtacag ctaaaatgac cttcggttct gttttctttg gtaatagcaa aggaacgctg
      481 aacaacgaca tgtctattaa caacccgtct gacggcgtga atatcgctct gcacaatatt
      541 gatggttcca ctatcaaaca ggttcaaatt aacaaccctg gcgatgtgta tactaaagcc
      601 ctggatgcaa cgacaaaatc tgctgtttat gattttaaag cgtcttacgt tcgtgccgtc
      661 gcagaccaaa cagcaactgc tggttatgta aaaactaaca ctgcatacac cattacttat
      721 cagtaagtat tcaccaatta aattgattga atgaatatac agggaatagt gttaataatt
      781 tctattttta tattattccc tgttttaatt aactctgtca gggatggttt atgtttttta
      841 acaccaaaca tacaacagct ttatgcttcg taacctgtat ggcttttagt tcatcgtcta
      901 ttgcggacat tgtcatttcg ggtactcgcg taatatataa aagcgatcaa aaaagtgtca
      961 acgtacgtct ggaaaataaa gggaataacc cgttgcttgt ccagagttgg ttagatactg
     1021 gcgataacaa cgctgagcct ggcagtatta cagtcccttt tactgccacg ccgccagtat
     1081 cgcgtattga tgccaaacgt gggcaaacaa tcaaattaat gtacacagcc agcacctcac
     1141 tgcctaaaga cagagagagc gtgttctggt ttaacgtact ggaagttcca ccaaaaccag
     1201 atgcagaaaa ggtcgcgaat caaagtctgc tgcaactggc atttcgcaca cgtataaaac
     1261 ttttctatcg cccggatgga ttgaagggaa atccctctga agccccgtta gccctgaagt
     1321 ggttctggtc aggttcagaa ggtaaggcgt cattgcgagt gaccaatcca accccttact
     1381 acgtctcttt tagcagtggt gatttagaag ctagcggtaa acgctatccg attgatgtga
     1441 aaatgattgc accatttagt gatgaggtca tgaaagtcac tggccttaat ggcaaagcaa
     1501 gttctgcaaa agtacatttt tacgccatta atgattttgg tggcgccatt gaaggtaatg
     1561 ccagtctgta atcaggcaag gatataattc cgcaggaagc ataacgtgac tataaaacca
     1621 actaaaaatt atcataatca tctgacccgt atcgctacgt tttgcgcgct gctgtattgc
     1681 aatagtgctt tctgtgctga acttgttgaa tatgaccata ccttcctgat ggggcagaat
     1741 gcctcaaata ttgatctcag ccggtatagt gaaggtaacc ctgctatacc gggtatgtac
     1801 gacgtcagtg tttatgtcaa cgatcaacct attattaacc aaagcattac atttattgaa
     1861 attgaaggaa aaaaaacgca caagcctgta tcacacttaa gaatttattg cagtttcata
     1921 taaattctcc cgatataaat aacgaaaaag ccgttctgct tgccagggat gaaacactcg
     1981 gcaattgcct taatttgacg gaaattatcc ctcaggcttc tgttcgttat gacgttaacg
     2041 agcaacgtct ggatatagac gttcctcagg catgggtaat gaaaaattac caaaactatg
     2101 ttgatccatc gttatgggaa aatggcatta atgcggccat gttgtcatac aacctcaacg
     2161 gatatcatag tgaaactcct ggtcgaagaa atgacagcat ttatgctgct tttaacggtg
     2221 ggatgaattt aggtgcatgg cgactgcgtg cctcaggcaa ctacaactgg atgaccgatt
     2281 ctggcagtaa ttatgatttt aagaatcggt atatacagcg tgatatcgcc tcgctgcgtt
     2341 ctcaactcat tcttggtgag tcttatacaa cgggcgaaac ctttgattct gtcagtatcc
     2401 gcggcattcg tttatacagc gacagccgca tgttgcctcc gacattagcg agttttgcgc
     2461 ctatcattca tggcgttgct aataccaacg ccaaagtcac tattacgcaa ggtggctata
     2521 agatttatga aacgactgtg ccgccaggcg ctttcgtcat tgatgatctg agtccgtcag
     2581 gttacggcag cgatcttatt gttactgtcg aagaatccga tggctcaaag cggacattct
     2641 cgcaaccttt ttcatcggtc gttcaaatgt tacgccctgg cgttggacgt tgggatatta
     2701 gcggcggcca ggtcttaaaa gatgatattc aggatgagcc taatttattt caggccagct
     2761 actactacgg tctgaataac tatctaac
//
LOCUS       DADMKF010000283         2739 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.288, whole genome shotgun sequence.
ACCESSION   DADMKF010000283 DADMKF010000000
VERSION     DADMKF010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2739
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.288"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(<1..1007)
                     /gene="shiA"
                     /locus_tag="JNC24_004129"
     CDS             complement(<1..1007)
                     /gene="shiA"
                     /locus_tag="JNC24_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate transporter"
                     /protein_id="HAY9160730.1"
                     /translation="MDSTLISTRPDEGTLSLSRARRAALGSFAGAVVDWYDFLLYGIT
                     AALVFNREFFPQVSPAMGTLAAFATFGVGFLFRPLGGVIFGHFGDRLGRKRMLMLTVW
                     MMGIATALIGILPSFSTIGWWAPILLVTLRVIQGFAVGGEWGGAALLSVESAPKNKKA
                     FYSSGVQVGYGVGLLLSTGLVSLISMMTTDEQFLSWGWRIPFLFSIVLVLGALWVRNG
                     MEESAEFEQQQHYQAAAKKRIPVIEALLRHPGAFLKIIALRLCELLTMYIVTAFALNY
                     STQNMGLPRELFLNIGLLVGGLSCLTIPCFAWLADRFGRRRVYITGALIGTLSAFPFF
                     MA"
     gene            1322..2374
                     /locus_tag="JNC24_004130"
     CDS             1322..2374
                     /locus_tag="JNC24_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HAY9160731.1"
                     /translation="MFLASLLRRIAFSYYDYKAYNFNIEKTDFVVIHIPDQIGDAMAI
                     FPVIRALELHKIKHLLIVTSTINLEVFNALKLEQTKLTLVTMTMQDHATLKEIKDLAK
                     NITQQYGTPDLCIEGMRKKNLKTMLFISQLKAKTNFQVVGITMNCYSPLCKNASRMDQ
                     KLRAPVPMTWAFMMREAGFPAVRPIYELPLSEDVLDEVREEMRSLGSYIAFNLEGSSQ
                     ERTFSLSIAENLIAKIQSETDIPIVIVHGPKGEDKARALVDCYNNVYRLSLPPSIKRS
                     AAIIKDAYIAITPDTSILHMASAYNTPVVAIYADYKTRWPAMADVSESVVVGQKIDNI
                     SLDEFAKALKSVLARI"
BASE COUNT          849 a          592 c          616 g          682 t
ORIGIN      
        1 gccataaaga aaggaaatgc gctcaacgtt ccgattaacg cacctgtgat ataaacccta
       61 cggcgaccaa aacgatcggc aagccaggca aaacagggaa ttgtcaggca gcttaatcca
      121 cctaccagca aaccaatatt aaggaaaagt tcgcgcggta gccccatatt ctgggttgaa
      181 taattaagtg caaaggcagt aacgatgtac atcgtcagca attcgcacag tcgtagcgca
      241 ataatcttca ggaaagcacc gggatgtcgt aacagcgctt cgataaccgg gatgcgtttt
      301 ttcgcggcag cttgataatg ttgctgttgt tcaaattccg cggactcctc catgccattg
      361 cgcacccaca atgctcccag taccagtacg atgctaaaca ggaaaggaat gcgccagccc
      421 cagcttaaaa actgttcgtc agtcgtcatc atactgatca atgaaaccag tccggttgaa
      481 agcagtaaac ctacaccgta gccaacttgt acaccgctac tgtaaaaggc ttttttattt
      541 ttcggtgcac tttcaacgga aagcaacgcc gcgcctcccc attcgccgcc gacggcaaat
      601 ccctgaatga cacgcagtgt caccagtaaa ataggtgccc accacccaat ggtcgagaat
      661 gaaggaagaa taccaatcaa ggctgtcgcg atgcccatca tccagacggt cagcattaac
      721 atgcgcttac gtcccagtcg gtcgccaaag tgaccgaaaa tgacaccgcc gagcggacgg
      781 aaaaggaatc cgacgccaaa ggtagcaaat gcggcgagcg ttcccatcgc cgggcttact
      841 tgcgggaaaa actcgcgatt aaacaccagt gcggcggtga tgccatagag taaaaaatca
      901 taccagtcga cgacggcacc agcgaagctg cctaacgcag ctcgtcgggc gcgacttaac
      961 gaaagcgtcc cttcatcggg acgagtggag atgagcgtgg agtccataat gatcctgttt
     1021 gtactgtctt tttataatta gtatggaaaa ggtaactcac attgccgtcg atctataaac
     1081 gaaccttatg agactacctc gacagagcgc atctgcaaat gattttatgg ctgattagcg
     1141 gcaacatgaa aaattcgatg gaagatgaaa gcgcaggagt tgctggtgtt gtttaaggat
     1201 taagcagagg acccacaggt tagagatatg aaacataaaa gtccgcatat gcacaaaatg
     1261 gcaccctgat gagtctgata gactgcattg cattataacc agtagggagg cgaggttagg
     1321 tatgttttta gcatcattgt tgagacgtat tgcatttagt tactacgatt ataaagctta
     1381 taatttcaat attgaaaaaa cagactttgt tgtcatccat attcccgatc agattggcga
     1441 tgctatggcc atctttcctg ttattcgggc gcttgaattg cataaaatta agcatctttt
     1501 aattgtaacg tcgacaatta atttagaagt ctttaatgcg cttaaacttg aacagactaa
     1561 attaacatta gtcacaatga ctatgcagga tcacgcaaca ttaaaagaaa taaaagattt
     1621 agcaaagaac ataacacagc aatacggtac gccggatctt tgtattgagg ggatgcgtaa
     1681 aaagaacctg aaaacgatgt tatttatcag tcagttgaaa gcaaaaacga attttcaggt
     1741 tgttggtata accatgaatt gctactcccc tttgtgcaag aacgcgtccc gtatggatca
     1801 gaaactccgg gctcccgtac ctatgacatg ggcatttatg atgcgtgagg cgggttttcc
     1861 agcagtcagg ccaatatatg aattgccact aagtgaggat gtactcgatg aggtgcgcga
     1921 ggaaatgcga tcgttaggat cttacattgc gttcaattta gaaggtagct cgcaggaacg
     1981 tacattttca ttatcgattg cagaaaatct aatagcaaaa attcaaagtg aaacagatat
     2041 accaatagtg atcgttcatg gacccaaagg tgaagataaa gccagggcat tagtggattg
     2101 ttataataat gtctaccgtt tatccttacc accctcgatt aaacgttcag cagcaatcat
     2161 aaaagatgct tatatcgcaa taactcctga cacctcaata ttacatatgg caagtgccta
     2221 taatacccct gttgttgcaa tttatgctga ttacaaaacg cgatggcccg caatggccga
     2281 tgtctcggag tcagtcgtcg ttgggcaaaa aattgacaat ataagtctgg atgaattcgc
     2341 aaaggcatta aaaagtgttt tggcgagaat atgagacagt acttatgcat agtaatgccg
     2401 atctgttatg aacggtggtg tcatcaatac tctgaggctg gtcaatcatc acagaaaacc
     2461 agcctttgaa tcaaagcagt actcaccgga tacgatcata gtcacatcga acgcaattct
     2521 ggcggtgccg catttttatt tattcatttt ctgtgagaga agggcaagct ctatagaggg
     2581 aatgtaaaaa agagagctcc gaatggagca ctctttaaga aattataacg gaaaatcaga
     2641 ggtttttata acatgtcgca taagcaaacc caagcttttc aaatacgctt tgatcacctg
     2701 ttgccaggga aactacgtat tgttcgcttg agtttgcgg
//
LOCUS       DADMKF010000284         2726 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.289, whole genome shotgun sequence.
ACCESSION   DADMKF010000284 DADMKF010000000
VERSION     DADMKF010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2726
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.289"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(423..680)
                     /locus_tag="JNC24_004131"
     CDS             complement(423..680)
                     /locus_tag="JNC24_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016063373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9160732.1"
                     /translation="MSDHVIECASRAGRDFSEFMKGEKGMMEALASVDEFGEQLRLNG
                     CVNHHFVSYMMRNSIMQAFMDMAKAERKEERRRKRAEAKAK"
     gene            complement(677..1174)
                     /locus_tag="JNC24_004132"
     CDS             complement(677..1174)
                     /locus_tag="JNC24_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001496757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAY9160733.1"
                     /translation="MKSLTLFNQPIRIGEDGMICLTDMWKASGKSESESPYHYLRNKQ
                     TKEFLAELEKNHESVVFTERGVHGGTYGGKFVAYDYAAWLNPGFKYAAYKVLDDYFTG
                     ELQHRNSLSAQLNMKCHEFDQKKDMASFCGQGLAAWRYTKPVLVAEINSLANQLQITI
                     PGLPG"
     gene            complement(1376..1834)
                     /locus_tag="JNC24_004133"
     CDS             complement(1376..1834)
                     /locus_tag="JNC24_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HAY9160734.1"
                     /translation="MSRVTAIISALVICIIVCLSLAVNHYRDNAIAYKEQRDNKASEL
                     EKANATIADMRKRQRDVAELDARYIKELADANATIESLRADVSAGRKRLQVAATCAKS
                     TTGASGMGDGESPGLTADAELNYYRLRSGIDKITAQVNYLQEYIRTQCLK"
     gene            complement(1831..2328)
                     /locus_tag="JNC24_004134"
     CDS             complement(1831..2328)
                     /locus_tag="JNC24_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HAY9160735.1"
                     /translation="MPPSLRKAVAAAIGGGAIAIASVLITGPGGNDGLEGVRHNPYKD
                     IVGVWTVCYGHTGKDIIPGKTYTEAECKALLNKDLATVARQINPYIKVDIPETTRCAL
                     YSFVYNVGAGNFRTSTLLRKINQGDIKGACDQLRRWTYAGGNQWKGLMTRREIEREVC
                     LWGKQ"
     gene            complement(2328..2543)
                     /gene="essD"
                     /locus_tag="JNC24_004135"
     CDS             complement(2328..2543)
                     /gene="essD"
                     /locus_tag="JNC24_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage lysis protein EssD"
                     /protein_id="HAY9160736.1"
                     /translation="MKSMDKLTTGVAYGTSAGSAGYWFLQLLDKVTPSQWAAIGVLGS
                     LVFGLLTYLTNLYFKIKEDKRKAARGG"
BASE COUNT          648 a          707 c          620 g          751 t
ORIGIN      
        1 atacagcgca tgatgtgtca ggcttgaata cctttatcct ttaaaaggga tatcagttaa
       61 gttatcccgt gtagggtata agccattatc aaagccactc tgtagggaat ggcttttgtg
      121 atggcatcac ttactcttta cgctgctatc ccactcatcc cggaattttg atgggttatt
      181 gaaaccttct gctgacataa caactccttc aatgtttggc tgaaattggg atgtctttcc
      241 atcagtccgc caccacaaag aatctttttt gccataaggc aggaggttca tctttcagtg
      301 gctgccggtg ttatttcccc acttactggc ttgggttgtt tcgtggtact gccgttaact
      361 ggtggcccag aataaattcc ggtttcatta tcaagcccac ccgtaaatgg gctttgtaat
      421 ggctacttcg cttttgcttc cgctcgctta cgccggcgct cttctttcct ctcggctttt
      481 gccatgtcca tgaatgcctg catgatcgag ttccgcatca tgtagctaac aaagtgatga
      541 ttgacacagc cgttgaggcg cagctgctcg ccaaactcat ccaccgaggc caatgcttcc
      601 atcatgccct tctcgccttt catgaactct gagaagtcgc gccccgctct ggaggcgcat
      661 tcaataacat gatcactcat cccggaagcc cggggatcgt aatctgcagc tggttagcca
      721 gggagttaat ctcagcgacc aacactggct tcgtatagcg ccatgctgcc agcccttgtc
      781 cgcagaagct cgccatatct ttcttctggt caaattcatg acatttcatg ttgagctgcg
      841 cacttaagct gttgcgatgc tgaagttctc cggtgaagta gtcatcgagg actttatagg
      901 ccgcgtactt gaacccgggg tttaaccaag ccgcataatc gtaagcaaca aacttcccgc
      961 catatgttcc accgtgtaca ccgcgctcag taaaaaccac agattcgtgg tttttctcca
     1021 gctcggctaa gaactctttg gtctgcttgt ttcgcaggta gtggtaaggc gattcagatt
     1081 cacttttacc actggctttc cacatatcag tgaggcagat catgccatct tcaccgatac
     1141 gaattggttg attgaagagg gttaatgatt tcatagcgtg tacctactct ttgaaatgaa
     1201 cctttgccgc acaggaaacc agcccaccga ggctcgccag cactaactgg tatcctcaaa
     1261 ggcccattcc aaaggggcag gttcggtgta aaaaacatgc gttgcggtac gcatttattg
     1321 caaaaagccc cgcatcgcga ggctcattaa atggactttg tgatttgcaa aaaaattatt
     1381 tcaggcattg cgtcctgatg tattcctgca ggtagttaac ctgcgcggtt atcttgtcga
     1441 ttccacttcg gagacggtaa taattgagtt cagcatctgc tgtaagtcct gggctttctc
     1501 catcgcccat gccgctggct ccggtcgttg actttgcaca ggtggcggcg acttgcaggc
     1561 gcttacgccc agcagaaaca tcagcacgga gactttcgat agtcgcgtta gcatcagcaa
     1621 gctcctttat gtatcttgcg tcgagttctg ctacatcacg ttgacgcttc cgcatgtcag
     1681 cgatggtggc gttcgccttc tccagttcac tggccttgtt atcgcgctgc tctttgtagg
     1741 cgattgcgtt atcacggtaa tgattaacag ccaatgacag gcagacgatg atgcagataa
     1801 ccagagcgga gataatcgcg gttactctgc tcattgtttc ccccacaaac agacttcacg
     1861 ctcaatctca cggcgagtca tcagtccttt ccattgatta ccgccagcgt atgtccagcg
     1921 ccgtagctga tcacatgcgc ctttaatatc accctggttt attttgcgaa gaagcgtcga
     1981 tgttctgaaa ttgccagcac ccacgttgta gacgaacgag taaagagcgc agcgcgttgt
     2041 ttccggtata tcgactttga tgtacgggtt aatttgtctg gcgacagtgg caaggtcttt
     2101 attcaggagg gctttgcact ctgcttcggt atacgtttta ccgggaatga tgtcttttcc
     2161 tgtatgcccg taacatacag tccatacacc aactatgtct ttgtaaggat tatgtctcac
     2221 accttccaga ccatcgttac cacctgggcc agtgattaac acagatgcta tagcaatagc
     2281 cccgccacca atagcagcag caacagcttt tcgtaatgat ggaggcatta tccacctctc
     2341 gcagccttgc gcttatcttc tttaatcttg aaataaaggt ttgtcaggta cgtcagcagg
     2401 ccaaatacca ggctacccag cactccaatt gccgcccact gtgagggcgt gactttatct
     2461 agcagctgta aaaaccagta cccggcacta cctgctgagg tgccataggc gacacccgtt
     2521 gttaacttat ccatggattt cataacccca cctcgcagac aaagcgggtg taaattgagg
     2581 gaatactacg aaacgtaaca gactcggagt cagtgaataa ctcaggtatt gggttatcag
     2641 ctaatatcga gactcaaaaa atggaaaaac ccgctcgacg gcgggtttaa gctgtgtgac
     2701 gaagtaacca ctcttaacag cataac
//
LOCUS       DADMKF010000285         2723 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.290, whole genome shotgun sequence.
ACCESSION   DADMKF010000285 DADMKF010000000
VERSION     DADMKF010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2723
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.290"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            30..2546
                     /locus_tag="JNC24_004136"
     CDS             30..2546
                     /locus_tag="JNC24_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HAY9160737.1"
                     /translation="MPQRHHQGHKRTPKQLALIIKRCLPMVLTGSGMLCTTANAEEYY
                     FDPIMLETTKSGMQTTDLSRFSKKYAQLPGTYQVDIWLNKKKVSQKKITFTANAEQLL
                     QPQFTVEQLRELGIKVDEIPALAEKDDDSVINSLEQIIPGTAAEFDFNHQRLNLSIPQ
                     IALYRDARGYVSPSRWDDGIPTLFTNYSFTGSDNRYRQGNRSQRQYLNMQNGANFGPW
                     RLRNYSTWTRNDQTSSWNTISSYLQRDIKALKSQLLLGESATSGSIFSSYTFTGVQLA
                     SDDNMLPNSQRGFAPTVRGIANSSAIVTIRQNGYVIYQSNVPAGAFEINDLYPSSNSG
                     DLEVTIEESDGTQRRFIQPYSSLPMMQRPGHLKYSATAGRYRADANSDSKEPEFAEAT
                     AIYGLNNTFTLYGGLLGSEDYYALGIGIGGTLGALGALSMDINRADTQFDNQHSFHGY
                     QWRTQYIKDIPETNTNIAVSYYRYTNDGYFSFDEANTRNWNYNSRQKSEIQFNISQTI
                     FDGVSLYASGSQQDYWGNNDKNRNISVGVSGQQWGIGYSLNYQYSRYTDQNNDRALSL
                     NLSIPLERWLPRSRVSYQMTSQKDRPTQHEMRLNGSLLDDGRLSYSLEQSLDDDNNHN
                     SSLNASYRSPYGTFSAGYSYGNDSSQYNYGVTGGVVIHTHGVTLSQYLGNAFALIDAN
                     GASGVRIQNYPGIATDPFGYAVVPYLTTYQENRLSVDTTQLPDNVDLEQTTQFVVPNR
                     GAMVAARFNANIGYRVLVIVSDRNGKPLPFGALASNDETGQQSIVDEGGILYLSGISS
                     KSQSWTVRWGNQADQQCQFAFSTPDSEPTTSVLQGTAQCH"
     gene            2557..>2723
                     /locus_tag="JNC24_004137"
     CDS             2557..>2723
                     /locus_tag="JNC24_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY9160738.1"
                     /translation="MKRAPLITGLLLISTSCAYASSGGCGADSTSGATNYSSVVDDVT
                     VNQTDNVTGRE"
BASE COUNT          778 a          657 c          630 g          658 t
ORIGIN      
        1 caaatataaa aaacacaggt catcagggaa tgccacaacg acaccaccag ggacataaac
       61 gcacaccgaa acagttggcg ctcatcatca aacgttgttt gccgatggtg ctcactggca
      121 gcggcatgct ttgcactacc gctaacgccg aagagtatta tttcgacccc attatgctgg
      181 aaactacaaa aagtggtatg caaacaaccg atctgtcacg tttttcaaaa aaatacgcac
      241 aactaccagg aacttatcag gttgatatct ggctgaataa aaagaaggtt tcacagaaaa
      301 aaattacatt taccgccaat gcagagcaac ttctgcagcc acagtttacg gtagaacaac
      361 tacgtgagct gggtattaag gtggatgaaa tcccggcgct ggctgaaaaa gatgacgata
      421 gcgtgatcaa ctcgcttgaa caaatcattc ccggtacagc agctgaattt gatttcaatc
      481 atcagcgact taatttgagc attccccaaa ttgcactgta ccgtgatgca agaggttacg
      541 tctccccttc tcgttgggac gatggtatac caacgctgtt taccaactac tcgtttacag
      601 gttctgataa ccgttaccgc cagggcaatc gtagccaacg acagtacctg aatatgcaaa
      661 atggtgctaa ttttggcccc tggcgattac gcaactattc cacatggaca cgcaacgatc
      721 agacatcaag ctggaatacc atcagtagtt atttacaacg tgatatcaag gcattgaagt
      781 ctcagttgct tctgggagaa agcgccacca gcggcagtat tttttccagc tacaccttta
      841 ctggcgttca actcgcttcc gacgataata tgttgccaaa cagccagcgc ggatttgccc
      901 caacggtacg cggtatcgca aacagtagtg caatcgtgac tatcaggcaa aatggttatg
      961 tgatctatca aagcaacgtg ccagcgggtg cctttgaaat taacgatctc tacccctctt
     1021 ccaacagcgg cgatttagaa gtcacgattg aagaaagtga cggtacgcaa cgtcgcttta
     1081 tccagcctta ttcttcatta cccatgatgc agcgacctgg gcatctaaaa tatagcgcga
     1141 ccgctggacg ctatcgcgct gatgcaaaca gtgatagcaa agaacccgaa tttgctgaag
     1201 ccacggcaat atatggtttg aataatactt ttacgctgta tggtggcctg ctcggttctg
     1261 aagattatta tgcgctgggg atcggtatcg gcggcacact tggcgcactg ggcgcgttgt
     1321 cgatggatat caacagagct gacacccaat tcgataacca gcactctttt catggctatc
     1381 aatggcgtac gcagtacatc aaagatatcc cggaaaccaa caccaatatc gctgtcagct
     1441 actatcgcta taccaacgat ggctatttta gttttgatga agccaatacc cgtaattgga
     1501 actataacag tcgccaaaaa agtgaaattc aattcaacat cagccagaca atatttgatg
     1561 gggtaagtct gtatgcctcc ggttcgcagc aagactattg gggcaataac gataaaaaca
     1621 ggaatatctc agttggggtt tccggtcagc aatggggaat tggttacagc ctgaattatc
     1681 aatacagtcg ctacacggat caaaataatg accgcgcact ctctttgaat ctcagtattc
     1741 cgttagaacg ctggttaccg cgtagccggg tttcctatca gatgaccagc cagaaagatc
     1801 gcccaaccca acatgaaatg cgtcttaatg gctcactgct ggatgatggt cgcctgagct
     1861 atagcctgga acaaagtctg gatgacgata acaaccataa cagtagcctg aacgccagtt
     1921 accgttcacc ttatggcacc ttcagtgccg gatacagcta cggtaatgac agcagccaat
     1981 acaattacgg cgttaccggc ggcgtggtta tccatactca tggcgtgacg ctctcgcaat
     2041 atctgggcaa cgcttttgcg cttatcgatg ctaatggggc ttctggcgtg aggatacaaa
     2101 actatccggg gattgctact gatccctttg gctatgcagt ggttccttat ctcacgactt
     2161 accaggaaaa ccgtctctcg gtagatacta cgcagctgcc cgataacgtc gatcttgagc
     2221 aaacaacaca gtttgtggtg cccaacagag gtgcaatggt agcggcgcgt ttcaacgcca
     2281 atatcggtta tcgcgtactt gttatagtca gcgatcgcaa cggtaaaccg ttgccctttg
     2341 gcgctcttgc cagcaacgat gagacggggc aacaaagtat cgtcgatgag ggcggcatac
     2401 tatatctctc tgggatatcg agtaaatcac aaagctggac tgtacgctgg ggaaatcagg
     2461 cagatcaaca atgtcagttt gcttttagta caccggattc agaaccaaca acctctgtat
     2521 tacaaggcac ggcgcagtgc cattaaggat aaaaaaatga aaagagcgcc tcttataaca
     2581 ggacttttgt tgatatccac atcctgcgct tatgcctcct caggagggtg tggagctgac
     2641 agcactagcg gtgcgacaaa ttacagcagt gtggttgatg atgttacggt gaaccagaca
     2701 gataacgtga caggacggga gtt
//
LOCUS       DADMKF010000286         2704 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.291, whole genome shotgun sequence.
ACCESSION   DADMKF010000286 DADMKF010000000
VERSION     DADMKF010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2704
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.291"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            <1..248
                     /locus_tag="JNC24_004138"
     CDS             <1..248
                     /locus_tag="JNC24_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9160739.1"
                     /translation="AALPVSWIQMLAGLALLSTIGGSLYQALHNERERERDAAVVAFL
                     VTASGLTLVGIGSAFWGLIAGGVCYVVLNLIADRNRY"
     gene            complement(288..1496)
                     /gene="tnpB"
                     /locus_tag="JNC24_004139"
     CDS             complement(288..1496)
                     /gene="tnpB"
                     /locus_tag="JNC24_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element transposase accessory
                     protein TnpB"
                     /protein_id="HAY9160740.1"
                     /translation="MKRLQAFKFQLRPGGQQECEMRRFAGACRFVFNRALARQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKDLERAYKNFFRKRAAFPR
                     FKKRGQNDVFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGTWY
                     ISIQTESEVSTPAHPSASMVGLDAGVAKLATLSDGTVFEPVNSFQKNQKTLARLQRQL
                     SRKVKFSNNWQKQKRKIQRLHSCIANIRRDYLHKVTTAVSKNHAMIVIEDLKVSNMSK
                     SAAGTVSQPGRNVRAKSGLNRSILDQGWYEMHRQLEYKQLWRGGQVLAVPPAYTSQRC
                     AYCGHTAKENRLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQSGRPLKQ
                     EPTEMIQATA"
     gene            1504..2005
                     /gene="tnpA"
                     /locus_tag="JNC24_004140"
                     /pseudo
     CDS             1504..2005
                     /gene="tnpA"
                     /locus_tag="JNC24_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604919.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(2023..2691)
                     /locus_tag="JNC24_004141"
                     /pseudo
     CDS             complement(2023..2691)
                     /locus_tag="JNC24_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
BASE COUNT          626 a          665 c          664 g          749 t
ORIGIN      
        1 tggctgccct gcccgtaagt tggatccaga tgctggcagg tctggcgctg ttgagtacca
       61 ttggcggcag tttgtatcag gcgctgcata atgagcgtga gcgtgagcgc gacgcggcgg
      121 tggtggcatt tctggtaacg gcaagtggat tgacgctggt cgggattggt tctgcgttct
      181 ggggattaat tgccggaggc gtttgttacg tggtgttgaa tttaatcgct gacagaaacc
      241 gatattgaca tcctccacgc cctgaaggac gtggatccct gctacgttca ggctgtcgcc
      301 tgaatcattt cggtgggttc ctgcttcaac gggcggcctg actgcaccat ctctccacag
      361 gcaagaacgg cgtgccccgc cgctaaaatg ttacgagcac catttacatc ggcgttcgct
      421 gtatatccac atacctggca tctgaattta ctttgtgaca ggcggttctc tttcgctgta
      481 tgaccacagt acgcgcaacg ctggcttgta tacgctggcg gaacagcaag cacctgaccg
      541 ccacgccaga gctgcttgta ctcaagctgg cggtgcattt cataccagcc ctgatccagt
      601 atcgaacggt ttaaacctga ttttgcccgg acattgcgcc ccggctggct gaccgtaccc
      661 gctgctgact ttgacatgtt gctgaccttc aaatcctcaa tgactatcat tgcgtggttt
      721 ttgctgacgg ccgttgtgac tttgtgaagg tagtccctgc ggatatttgc gatacaggaa
      781 tgcagtcgct gtattttgcg tttctgcttc tgccagttgt tgctgaattt gaccttgcgg
      841 cttaactgtc gctgaagtct cgccagcgtc ttctggtttt tctgaaaact gtttacaggc
      901 tcaaagactg tgccatctga cagcgtggcg agtttagcca cgccagcatc cagcccgacc
      961 attgatgctg aagggtgagc cggagtggat acttcacttt ctgtctgaat actgatgtac
     1021 cacgtaccgc aggactggct gacagtgaca tttttcacaa cacccgtgac ctgacggcta
     1081 ttccggtagc gcatccagcc cagtttcggc agaaaaatac ggctgttttc ctgatcgagc
     1141 ttaacgccct gcgggtagcg gaatacatca ttctgtcccc gctttttgaa tcggggaaaa
     1201 gcagcccgct tccggaagaa gtttttgtaa gcccgctcaa ggtctttcag tgactgttgc
     1261 aatggctgtg agggagaatc tttaagccat tgcgtttcag tggcattttt ccactcaacc
     1321 agccaggaag ccattttccc gtaagggatg tatttattac cggcctcatg attctcattc
     1381 tgacgtgcca gagcacgatt gaaaacgaaa cgacatgcgc ctgcgaagcg cctcatttca
     1441 cactcctgtt gaccacctgg tcttaactgg aatttaaatg cctgtaatcg tttcacaggt
     1501 caaatgatac tttggtctgt gaaaaaagaa accgatatcc ggcgtggaag gcattgtgtt
     1561 ttcctgatgc atgttcacct ggtctttgtc actagatacc gacgccagat tttttgacgc
     1621 gacagaaaaa ctacgcactt acttttcaaa tgtatgtgct tattttgaag ctgaactggt
     1681 tgaaatggat ggcgaaccag atcacgtcca tttgttaatt aactaacctc ccaaactggc
     1741 gatatccagt ctggtaaaca gcctcaaagg cgtatcgggt aggttactgc gacgagatcg
     1801 accagatatt gcagtcaggt attactacaa aggcgttttg tggagtcctg gctattttgc
     1861 cagtagctgc ggaggtgtgc caatatccgt catccgccaa tacattgaac aacagcaaac
     1921 acctggtcag gtggaaaacc gcgccttata tccccgccct gaaggatggg gttttacggc
     1981 gcaccggata aaaagcgcca tctaacgatg gcgcgtctgg aactactttt tgttaacgtc
     2041 aaacatggtg gcatcggtcg ccatgtcatc aataacttgt ttaatatttt cgaaagccat
     2101 tggcgtactt tcgttattca ggtctttacc ttcgccctga cgaacgactt tgataaccgg
     2161 tttattagtc gctgcatcaa tcagctcacc ttcaaaatag agtcgagtat ccatggtacg
     2221 gtggcctgta gccatttgcg tccccgcaac cactaatgca acaggaacca cttcatagaa
     2281 ttgcagccct tctttgctgg tatctacacc ggtaatggcc ccacggaaaa tcagactacg
     2341 cggcccagcg gtggtaacca gtggtttacg ctgcgctatc gcttctttca tttcggtgtt
     2401 ggtatagttc aaaattttat ccagaacttt ctgccctacc taggtagaag gttttggcac
     2461 cggataataa gtgattgggt tccagacgat gctgtcatat ttgctttgat caaaactcgg
     2521 gtctacccaa cgtaaaacag gtttacctgt tgctgaggtt gtttctttta aatcagagta
     2581 attgtttaaa aaaccagaat atttatctgg ctgggtgatt ttagaggcac aaccagacag
     2641 agccaacaag ccgcataaag ctgcaacttt cgcaaatgat gtggtacgca tgaatagttt
     2701 cctg
//
LOCUS       DADMKF010000287         2690 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.292, whole genome shotgun sequence.
ACCESSION   DADMKF010000287 DADMKF010000000
VERSION     DADMKF010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2690
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.292"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            230..769
                     /locus_tag="JNC24_004142"
     CDS             230..769
                     /locus_tag="JNC24_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015870862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HAY9160741.1"
                     /translation="MSHGGWKEISHPQTLFTRQKGDGWTVVPKNLRIDFYTKDNDFTK
                     GLSVLSEVNKRHTEAQKGLTPSLNISKDDLKVLANARNIPQSKLEEEMMSQAIYRKEY
                     ATSNEKIKNYALYYHEQAQNIIQRHQDGLGNKNIDIAFITDKNHKKHLSDIFKVINES
                     GVKYDVIHFGACRVNREEK"
     gene            complement(995..1318)
                     /locus_tag="JNC24_004143"
     CDS             complement(995..1318)
                     /locus_tag="JNC24_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nikA protein"
                     /protein_id="HAY9160742.1"
                     /translation="MTKRSGSNTRRRAISRPVRLTAEEDQEIRKRAAECGKTVSGFLR
                     AAALGKNVNSLTDDRVLKEVMRLGALQKKLFIDGKRIGDREYAEVLIAITEYHRALLS
                     RLMAD"
     gene            1604..1798
                     /locus_tag="JNC24_004144"
     CDS             1604..1798
                     /locus_tag="JNC24_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HAY9160743.1"
                     /translation="MNKQQQTVLNMAGFIKSQSLTLLEKLDALDADEQAAMCEKLHEI
                     AEELQNSIQTRFEAESGTGV"
     gene            complement(2119..2386)
                     /locus_tag="JNC24_004145"
                     /pseudo
     CDS             complement(2119..2386)
                     /locus_tag="JNC24_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000844.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          857 a          665 c          579 g          589 t
ORIGIN      
        1 aaaaaaaaca ataaaaaaca ccgcaaaaat ggattgttaa caaatgaaaa tactcaattc
       61 gaatctcata acaaacatat catcaacaat aagaaatcac cttctaacat catccaaaaa
      121 tataaaaaca cacccatcac caaacatgga cgtaacaagt catcgtcgtt tattccagaa
      181 agacggaata ggattgagcc aaacaggaaa agcaacacaa cttgtcatta tgtcgcatgg
      241 tggatggaaa gagatctccc atccacaaac actattcacg cgtcaaaaag gcgatggatg
      301 gactgtagtc cccaaaaacc taagaattga tttttataca aaagacaacg acttcacaaa
      361 gggactaagc gttctttcag aggtaaacaa acgccatact gaagctcaaa aagggttaac
      421 accatcacta aacataagca aagatgacct aaaagttcta gcgaacgcgc gcaatatacc
      481 tcaatcaaaa ttagaagaag aaatgatgtc acaagccata tatagaaaag aatacgcaac
      541 aagtaatgag aagataaaaa actatgctct atattaccat gaacaagccc aaaatattat
      601 acaaagacat caagatggct taggaaacaa aaatattgat atagcattta taactgataa
      661 aaatcataag aagcaccttt ctgacatatt taaagtaata aatgaatcag gagtaaaata
      721 tgatgtaatt cactttggcg cttgccgagt aaatagagag gagaaataat catcatttac
      781 cataatccct taattgtacg caccactgaa acgcgctgaa cgcgattctg acgcagacac
      841 cacaaaaata gatgttttac cccggaaaaa caggaaggat gcgtcaggat cgttttcagc
      901 tcgctgcata gctatgcatg aaagcgaatg gagatcactt tgggagctta ccgtgttcat
      961 accgtctgtt ttcaacagtt ttctccccgg gaagctaatc tgccataagc ctggataaca
     1021 gggcacggtg atactccgta atagcgatca gcacctccgc atactccctg tccccgatac
     1081 gcttgccgtc gataaagagt tttttctgta acgcccccag tctcataact tctttcagca
     1141 cccgatcatc agtcagtgag ttaacgttct taccgagagc tgccgcccgt aaaaaaccag
     1201 aaacggtctt gccgcattca gcagcccttt ttctgatttc ctggtcttct tctgccgtca
     1261 gacgaacagg gcgactgata gcccgcctgc gcgtattact tccacttcgt tttgtcataa
     1321 aacatggctc cgtatctgac acggggtgtc ggggcgaagc cctgaccagg gtaattgtaa
     1381 tagcgtgcat gtatgcgcgg tataacaatt acacatcctg tcctgtcagc aagttcgaat
     1441 taccgacaaa ggcacggcag tttttttggc agtgacagta ttgtcaggca gaaatacccg
     1501 tcaatgcgtc agacggcaga ataccggcgg taaaacagtc gaaaagtggt acgcgatata
     1561 atcacacaac gcagatgaca gacagaacgg agaaaacaga aagatgaaca agcagcagca
     1621 aaccgtactc aacatggcgg gattcataaa aagccagagc ctgacgctgc tcgaaaagct
     1681 ggacgcactc gatgccgacg aacaggccgc catgtgtgag aagctgcacg aaatcgcgga
     1741 agaactccag aacagcatac agacacgctt tgaagcggaa agtggcaccg gggtataaca
     1801 gtgtatcctg ctccagacag gatgcagaac ggtataccgg tgcgtaagga gaaaatgtca
     1861 cataaggcgc tcttccgctt cctcgctcac tgactcgcta cgctcggtcg ttcgactgcg
     1921 gcgagcggtg tcagctcact caaaggcggt aatgcggtta tccacagaat caggggataa
     1981 cgccggaaag aacatgtgag caaaaagccg aaggcaggga caaaggccgc gcaggtggcg
     2041 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     2101 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     2161 cgctctcctg ttccgaccct gccgtttacc ggatacctgt ccgcctttct cccttcggga
     2221 agcgtggcgc tttctcatag ctcacgctgt tggtatctcg gcccggtgta ggtcgttcgc
     2281 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     2341 aactatcgtc ttgagtccaa cccggtaaga cacgaattat cgccactggc agcagccact
     2401 gataaccgaa ttagaggagt aagacttgaa gtcatgcgcc tgttaaggct aaactgaaag
     2461 aacaagtttt ggtgactgcg ctcctccaag ccagttacct cggctcaaag agttggtagc
     2521 tctgagaacc ttcgaaaaac cgccttgcag ggcggttttt tcgtttacag gcagcagatt
     2581 acgcgcagaa aaaaaggatc tcaagaagaa cctttaattt tttttcacac ttggtatcct
     2641 actttaaccc ataaaataaa cacttacacc gacacaccct taaacaaaag
//
LOCUS       DADMKF010000288         2644 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.293, whole genome shotgun sequence.
ACCESSION   DADMKF010000288 DADMKF010000000
VERSION     DADMKF010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2644
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.293"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            12..143
                     /locus_tag="JNC24_004146"
     CDS             12..143
                     /locus_tag="JNC24_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001344347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA invertase"
                     /protein_id="HAY9160744.1"
                     /translation="MLIGYVRVSTNDQNTDLQRNAFICAGSELIVERMGSWPEVVMA"
     gene            403..1398
                     /gene="pgl"
                     /locus_tag="JNC24_004147"
     CDS             403..1398
                     /gene="pgl"
                     /locus_tag="JNC24_004147"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HAY9160745.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESALPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTCWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDHSGKYLIVAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            complement(1439..2392)
                     /locus_tag="JNC24_004148"
     CDS             complement(1439..2392)
                     /locus_tag="JNC24_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308823.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY9160746.1"
                     /translation="MKHELSSMKAFVILAESSSFNNAAKLLNITQPALTRRIKKMEED
                     LHIQLFERTTRKVTLTKAGKRLLPEARELIKKFDETLFNIRDMNAYHRGMVTLACIPT
                     AVFYFLPLAIGKFNELYPNIKVRILEQGTNNCMESVLCNESDFGINMNNVTNSSIDFT
                     PLVNEPFVLACRRDHPLAKKQLVEWQELVGYKMIGVRSSSGNRLLIEQQLADKPWKLD
                     WFYEVRHLSTSLGLVEAGLGISALPGLAMPHAPYSSIIGIPLVEPVIRRTLGIIRRKD
                     AVLSPAAERFFALLINLWTDDKDNLWTNIVERQRHALQEIG"
BASE COUNT          673 a          588 c          648 g          735 t
ORIGIN      
        1 ggggatttac catgcttatt ggctatgtac gcgtatcaac aaatgaccag aataccgatc
       61 tacaacgtaa tgcgtttatt tgtgccggaa gtgagttgat tgttgagcga atgggaagtt
      121 ggccagaagt tgttatggca tagtgtttac ggtcgttgat acaggtaaac aaaagcgggg
      181 agagtcatac tccctgaata gtcgcgaaaa tatctgtggc attgtcaaat tcgtgtcggt
      241 gactaatatt ttagtaatat gccattcgaa atggagtgct aacttattga tttaattgat
      301 gtgcgatatt gtattttatc gtgttttgtt gtctgttttt tatccagtaa gtgttgttat
      361 gtgtatgaaa gataagggat ttatcatttt tgaggtgtgt tcatgaagca aacagtttat
      421 atcgccagcc ctgagagcca gcaaattcac gtctggaacc tgaatcatga aggcgcactg
      481 acgctgacac aggttgtcga tgtgccgggg caggtgcagc cgatggtggt cagcccggac
      541 aaacgttatc tctatgttgg tgttcgccct gagtttcgcg tcctggcgta tcgtattgcc
      601 ccggacgatg gcgcactgac ctttgccgca gagtctgcgc tgccgggtag tccgacgcat
      661 atttccaccg atcaccaggg gcagtttgtc tttgtaggtt cttacaatgc gggtaacgtg
      721 agcgtaacgc gtctggaaga tggcctgcca gtgggcgtcg tcgatgtggt cgaggggctg
      781 gacggttgcc attccgccaa tatctcaccg gacaaccgta cgctgtgggt tccggcatta
      841 aagcaggatc gcatttgcct gtttacggtc agcgatgatg gtcatctggt ggcgcaggac
      901 cctgcggaag tgactaccgt tgaaggggcc ggcccgcgtc atatggtatt ccatccaaac
      961 gaacaatatg cgtattgcgt caatgagtta aacagctcag tggatgtctg ggaactgaaa
     1021 gatccacacg gcaatattga atgtgtccag acgctggata tgatgccgga aaacttctcc
     1081 gacacctgtt gggcggctga tattcatatc accccggatg gtcgtcattt atacgcctgc
     1141 gaccgtaccg ccagtctgat taccgttttc agcgtttcgg aagatggcag cgtgttgagt
     1201 aaagaaggct tccagccaac ggaaacccag ccgcgcggct tcaatgttga tcacagcggc
     1261 aagtatctga ttgtcgccgg gcaaaaatct caccacatct cggtatacga aattgttggc
     1321 gagcaggggc tactgcatga aaaaggccgc tatgcggtcg ggcagggacc aatgtgggtg
     1381 gtggttaacg cacactaacc gctgatttac ccggcgcagt ctctcctgcg ccggtgtatt
     1441 aacctatctc ctgtaacgca tgtctctggc gttcgacgat attggtccac aaattgtctt
     1501 tatcgtcagt ccacaaatta atcagcaagg caaaaaagcg ttctgctgcc ggagaaagca
     1561 cggcatcttt gcggcgaata atccccaatg tccgacgtat aacgggttcc accagtggaa
     1621 taccaataat cgaagaatag ggcgcatggg gcattgccag accaggaagc gccgaaatcc
     1681 ccagtcccgc ctctaccagt cctaatgacg tcgaaagatg acgcacttcg taaaaccagt
     1741 ccagcttcca gggcttgtcg gccagctgtt gttctatcaa cagtcggttg ccgctggagg
     1801 agcgtacgcc aatcattttg taacccacta actcctgcca ttctacaagc tgctttttgg
     1861 ccaatggatg gtcacgccga caggccagca cgaacggctc gttgaccagt ggggtaaaat
     1921 caatggatga atttgtgacg ttgttcatgt ttatgccaaa gtctgattcg ttacacagca
     1981 ccgattccat gcaattattc gtgccttgtt ccagaatccg cactttaata ttgggataca
     2041 gctcattaaa tttaccgatt gccagcggta aaaaataaaa tactgcggtc ggaatacacg
     2101 ctaacgtcac cataccacga tgataagcat tcatatcacg aatattaaaa agcgtttcat
     2161 cgaatttctt tattaattcc cgcgcctccg ggagcaacct ttttcctgct tttgttaacg
     2221 taaccttgcg agttgtacgc tcaaatagtt gaatatgtaa atcttcctcc atctttttta
     2281 ttcttcgcgt taatgcaggt tgcgtaatat tgagtaattt agcggcattg ttaaacgaac
     2341 tggactctgc cagtatgaca aatgccttca tacttgataa ttcatgcttc attattactc
     2401 cggaaaatgg aagcgacgat tttgggtggc tggccgttaa aaattttaac tgcatttagc
     2461 caacttaaat taatgaaaaa atgttattaa tcgttgagct aaagtcatta gagatgcttt
     2521 gcccttaatg taaccatatc gcaataagtt atgtttttaa attgagggca ttattatgaa
     2581 aaaaataccc tgggtaatga ctccaactta ttgatagtgt tttatgttca gataatgccc
     2641 gatg
//
LOCUS       DADMKF010000289         2610 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.294, whole genome shotgun sequence.
ACCESSION   DADMKF010000289 DADMKF010000000
VERSION     DADMKF010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2610
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.294"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            1..1363
                     /locus_tag="JNC24_004149"
                     /pseudo
     CDS             1..1363
                     /locus_tag="JNC24_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
     gene            1535..1642
                     /locus_tag="JNC24_004150"
     CDS             1535..1642
                     /locus_tag="JNC24_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9160747.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(1695..2453)
                     /locus_tag="JNC24_004151"
     CDS             complement(1695..2453)
                     /locus_tag="JNC24_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HAY9160748.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
BASE COUNT          779 a          582 c          542 g          707 t
ORIGIN      
        1 cttgattcat tgagaaatgg aaaaggcaaa gtcaacctga ttaaacacta ctcctccgtt
       61 gaatccatac agcagcatgt ccccttagtc cgggacgcgg agttcagagc attacttcgc
      121 catcctcctg cagggagtcg cgttatcgcg agtaaggatt ttggcttcgc tttagatatt
      181 ttcttttgtc gaatgatggc aaacaatgtc agtcatatgt ccgcgatttt atatatagac
      241 aatcatactt tgtcagtaag gctacgaata aagcagtcag tgtatggaca attaaattat
      301 gttgtgtctg tttacgaccc gaacgatacc aacgttgccg tcagaggcac ccacagggca
      361 gcacggggct ttctctcgct tgataagttc atcagttcag ctcccgatgc tcagacctgg
      421 gctgataggt atgttcgcaa ctgtgcaatt gctattcttc ctctattacc tgagggagtt
      481 ccagttgcta ttttagcggg tatcacgaca cgaatgccat ttgcccctat acatccatcg
      541 gcaatgttgt taataatggc cacaggccag actcaatagc ttattacact attcaaacag
      601 ttgcccatac tccctgaaaa agaaatcatt gaaataataa ctgcgcagaa tagcgttggt
      661 acacctgctt tatttctggc tatgatgaac ggacaaactg acaacgtaaa aatatttatg
      721 caagaaattc agtcactggt atacaatcat atcattcatg aagataatct ggttaaatta
      781 ctgcaaacta aaagtgctaa cgaaacacct ggactctata tctccatgtt gtatggattc
      841 gatgaaataa tcgatatctt tctgaatgca ttaaccactc caatagcaca agaactttta
      901 aacaaaaaaa atggtgatga gtattttagc catgaaaata catgatggtg agccaggatt
      961 atacgccgca atggaaaata atcacccttt gtgtgtcaca cggttcctct ctaaaattaa
     1021 tggcatcgcc tttaaataca agttgagcaa agctaacatc atggatttat taaaaggagc
     1081 tacagcacag ggaacccctg cattatacat cgccatgagc aagggtaatg aagacgtcgt
     1141 gttatcttat atatcgacgc tgggtgcttt tgcaaaaaaa cattctttta gtcaacatca
     1201 gttatttaca ctattggccg ctaaaaatca tgacaacatg tcagctgttc atatagccat
     1261 tcatcataag cattataaaa ctgtagaaac atattatgct gctataaatg tcatcagcca
     1321 aagcctgagt tttagtgctg atgaaataaa gacgtattta taaccagcta tttacgatat
     1381 atatcgatac ataattttat ttttttcata aaacgcagaa acagtgacca tgtgcacaac
     1441 gccacccgac ggctgtaata acaaaaaatc ccttgatgcc tatccctttt gttacactcc
     1501 gttatcacgc acaagagata tgcaggacac tggtatgccg actaaacgct ttgataaaaa
     1561 acactggaag atggtggtgg tgctactggc aatctgtggc gctatgttgt tgctacgttg
     1621 ggcagcaatg atttggggct gagcagtgac aaacgaagac agccagaccg cataacacgg
     1681 tccggcagat aattttacat gctgtagccc agcgataagg ttgtccgacg atcggtatgt
     1741 tccggtgcag actctggagg ttcagagttc catgtgacgt tgtaagccac cttcagtcca
     1801 aaatgttcat taatagcaac atttaatgcg ctttcggagt tcagcgttgt atcttccgcg
     1861 ccaaagaccg aaacaccctg cgtaaattta gcgttgtcag tcaactgcca tgcataagcc
     1921 ccggaggcat agcccagcgg ctgggtttca ctggcattgt cggtatattt gtcgtaacgc
     1981 acacctgggc cgaattcaaa gcggaaactg tgtaccgggc cattcaaaaa ctggcgacca
     2041 taacccgcgg tcaacacatc gcgctctcga tagccgttat aacggtctgt cagccagctt
     2101 gcctgaccaa ataaatagtc ataatcagtt aaattaaaac ggctacgccc gcccgccgca
     2161 tatttttctg aagaacgctc atcattagaa gaagtattac tggcgttccc ccacagcgac
     2221 caggcagtgg tttgtccata ccaggtcatg gtggtgtcag ccgtaagtga ggagcttttc
     2281 gtattgcctg attgtgcaag atatcctgcg ttcagattac cttcgaaagg ttttttagcg
     2341 ctggcaggat cgtccatgac agtaaaaacg gaatcatcgg cagctgcatt cagtgacgca
     2401 aacatgcccc ccgccaacat aacgatggca ggaactgtct tcaaaagctt catttatcaa
     2461 gagtccgtac aacaaaaaaa gagaccatcg cggtcccgga aactttctta aggatcaaag
     2521 attagcgtcc ctggaaaggt aacgaattat aaaaaggcgc gaataactta gcaatgtatt
     2581 cttatttcat tttttgaata agcatgtggc
//
LOCUS       DADMKF010000290         2580 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.295, whole genome shotgun sequence.
ACCESSION   DADMKF010000290 DADMKF010000000
VERSION     DADMKF010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2580
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.295"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(880..1431)
                     /locus_tag="JNC24_004152"
     CDS             complement(880..1431)
                     /locus_tag="JNC24_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAY9160749.1"
                     /translation="MAILGYCRVSTDDQSITNQQMQIEEAGYNIAKWFTDEAVSGSVK
                     ASLRNGFSRLLAYAREGDTVVVVAVDRLGRDTIDVLSTVKALQAKGVTVISLREGFDL
                     SSAMGEAMLGIMSTLAQLERSLIAERRKAGIERAKAEGVHMGRPVKASSEAVQTLISQ
                     GKTRLQIQEELGISRATYYRLAK"
     gene            2230..2526
                     /locus_tag="JNC24_004153"
     CDS             2230..2526
                     /locus_tag="JNC24_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009448315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HAY9160750.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
BASE COUNT          743 a          541 c          492 g          804 t
ORIGIN      
        1 tttgtttaaa agcatatcaa acgatacatt ttattgattt aggtcaaaga caaaagcatt
       61 gacaaaatgg taagattgtt cacatgacct tacttaacag acttttaaga gactttgaaa
      121 cagcctgttt aacaggtcac tacctctggt cttatcgttc ccttctttct aggtggttat
      181 ctgttaagag tttttcagtt tttattctta agataattgt taagatgata ctctcccttc
      241 gggttatatc gtatctcttc tcccgttaaa tatcccttca cagtcagact aaataacacc
      301 tgtgtagttc ttggtagaat ctttcaaggt aacttagctc tgagtttatg aagttgacct
      361 atgagataga ctctaccgaa tctgttagtc aattgttgct taggtgctgc taaggcttac
      421 ttagtggaaa gagaggggat atgtgaccat aggggaaccc ctcctgttaa aagactactt
      481 agaagtctga ggttttagta aaatctgtga atatggcttc tcagacgctc tgtaacgcat
      541 tctaagcgat tttacccctt tattttaagg tggttagtac atccctatag gttaccccta
      601 aaaatggggt taaaactcgc tacagagctt tctatgagat tagcttctaa tcgaatcaac
      661 tatgaggttt ggtaggattg tgcaaaatct gtacagtaac ttttcagaat ctgaaaattt
      721 ttataaaaaa tgactagccc tctttgaatc tcatcttccc cgaatcttca aaaatatctg
      781 acagatgtct gtagctgcta agtacccgtc acaccagcta tgtgaccccc ttggcaaaaa
      841 agccaaatat cagctacatc catgtaacta ttcagtaact tatttagcaa gtctgtaata
      901 agttgctcta gagattccta actcttcctg aatctgcaac ctcgtcttac cttgtgaaat
      961 aagcgtctgt acagcctctg aagaagcttt tacaggcctc cccatatgta ccccttcagc
     1021 tttagcacgt tcaataccag cctttctacg ctctgctatc aagcttcttt caagctgtgc
     1081 caacgttgac ataatgccta acattgcttc acccatagcg cttgataagt caaacccttc
     1141 tctaaggctg ataactgtca cacctttagc ctgtagcgct ttaaccgttg ataacacatc
     1201 aattgtgtca cgtccaagac ggtctacagc taccacaact acggtgtcac cttctcgtgc
     1261 ataagctaat agtctgctga aaccgtttct taaagaagct ttgacagaac cactgacagc
     1321 ttcatcagtg aaccatttag caatgttgta ccctgcctct tcaatttgca tttgctggtt
     1381 agtgatggat tggtcatctg tactcaccct gcaatatcca agtatcgcca taatgttccc
     1441 cttagaagtc tcaaaagtta gtctcataag tttagctgtc tcataatcca aaaacaacct
     1501 tttgagacta cttgagggtg cattctaccg ttacgttgaa agtgtctcaa aaggaagtcc
     1561 ttttaagact ggttatgaag gagctatgag gttgattagg ggttggtaca agcccaacta
     1621 accgaatcaa ctatgaagat tttaggaggg taactgtaac catcactttc taactttcac
     1681 gtaggaaatt gttaagtggt ttgatagtta caccatgtga ccaagcatct atatatcgtt
     1741 atatagccct gtatatagca ctatatgtag tgtcgagaga ggaacgacta acacgataca
     1801 gtttaccctg aaaagagact tcacagattc tttatatatt tcaataagtt agggtaaaaa
     1861 gggactatac cgagactgta tagaatctgt gtagaattgc atagatactt ggaaatctac
     1921 tattaaattg catagggact cacctgatat tgtcctgttt ttaacaggtc tcctagaaaa
     1981 ccgaatagac ttcattcaag ggtcattgaa gtcataatca tttcagtaga aaaaccaagt
     2041 ctcgatttta aatgcaaata gcaatcatat tcaataaggc aagacgatca ggatcagtga
     2101 aaatgtcatt tttggcgtaa ttgaccttac tttatgcatg gtcttaacaa agacatcatc
     2161 tttccgttgc agatcttcgc cctttcaaat aacgtacttt acaactttcc cgaacaagga
     2221 gttgtgcccg tgttgtatgt tatttacgct caagacaaag ctgatagcct cgaaaaacgc
     2281 ctttccgttc gtccggcaca tttagcacgt ttacagttac tgcatgatga aggtcgtttg
     2341 ttgacagctg gtccaatgcc agcggtagac agtaacgatc caggggctgc gggatttact
     2401 ggctcaaccg ttattgctga atttgaatcg ctggaagcag ctcaggcctg ggccgatgct
     2461 gacccgtacg tagcggcagg tgtctatgaa cacgtttcag tgaaaccatt taagaaagtg
     2521 ttctgaatag agattcaata tttaagggca cggtttttgc aaccgtgttc ttgtttggcc
//
LOCUS       DADMKF010000291         2577 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.296, whole genome shotgun sequence.
ACCESSION   DADMKF010000291 DADMKF010000000
VERSION     DADMKF010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2577
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.296"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            <1..397
                     /locus_tag="JNC24_004154"
     CDS             <1..397
                     /locus_tag="JNC24_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3289 family protein"
                     /protein_id="HAY9160751.1"
                     /translation="KECIENGINSTPSTGYQPLILQSIKTKLLSSRLNKFNDFEDSFN
                     GLGISVHDISAQKISLLSFQKYAIGWSATIHFVAQDHFGLDVTDIKNKTYSKYRFFRI
                     WFFLQRHKDFAFKPFFTNFNTIERIENYL"
     gene            394..873
                     /locus_tag="JNC24_004155"
     CDS             394..873
                     /locus_tag="JNC24_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF943 family protein"
                     /protein_id="HAY9160752.1"
                     /translation="MKSKLQKIILCLFLLCCIYNLWTLRPVQILYTYSDAGNSVFLVV
                     DHLPWTDSDKINWYLKHQNEIKNQHPLPEGSWHTWYVIDIGNGFTDYKKYIEGPYEDL
                     YCFPTIKSNDNCIVKNYLMVINEYPYRNTHIGINDFTEYQLTQENKIERVFNPHDFK"
     gene            complement(927..1877)
                     /gene="corA"
                     /locus_tag="JNC24_004156"
     CDS             complement(927..1877)
                     /gene="corA"
                     /locus_tag="JNC24_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HAY9160753.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            2247..>2577
                     /locus_tag="JNC24_004157"
     CDS             2247..>2577
                     /locus_tag="JNC24_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9160754.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLALTLLPLFAVAADDCALSDPMLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLVDMNSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQ"
BASE COUNT          753 a          590 c          560 g          674 t
ORIGIN      
        1 caaagaatgt atcgaaaatg gcattaattc aactccctcg acaggatacc aacctcttat
       61 cctgcaaagc attaaaacca aattactttc atcaagacta aataaattta atgacttcga
      121 ggacagtttt aatggcttag gaatcagtgt tcatgatatt tcagctcaaa aaatatctct
      181 tttaagtttt caaaaatacg ctataggatg gagtgcaaca attcattttg tagcacagga
      241 ccacttcggt cttgatgtaa ctgatatcaa aaacaagact tacagcaaat atagattttt
      301 tcgcatatgg ttctttttac aacggcataa agactttgct ttcaaacctt ttttcaccaa
      361 ttttaacact atcgaaagaa tcgaaaatta tctatgaaaa gcaaactcca gaaaattata
      421 ttatgtttat ttcttctatg ctgcatctat aatttatgga cattgcgtcc tgtacagatt
      481 ttgtatacat actctgatgc tggcaattca gtctttctgg tcgttgatca tctgccatgg
      541 actgacagcg ataaaataaa ttggtatctg aagcatcaga atgaaattaa aaatcaacat
      601 cccctaccag aaggtagttg gcatacctgg tatgtcattg atatcggtaa tggcttcact
      661 gactataaaa aatatatcga agggccgtat gaagatctct actgtttccc gacaattaag
      721 agcaatgata attgcatcgt gaaaaattac ttaatggtta ttaatgaata cccttatcgt
      781 aacactcata ttggtattaa cgattttact gagtatcaat taacacaaga aaataaaata
      841 gagcgagttt tcaatccgca tgactttaaa taagacaatt tttagaaata agccggataa
      901 aaaaatagct ttatccggca ctcatattac agccagttct tgcgcttaaa gtacagatac
      961 ggtgccaggc ccgcgaggat cataaagata atcgcgcctg ggtagccgaa actccacttc
     1021 agttctggca taaactcaaa gttcatccca tagctggaag caacgagcgt cggcggcagg
     1081 aataccacgg ataccaccga gaagattttg atgatgcggt tctgctcgat gttgataaaa
     1141 cccatcgccg cctgcatcag gaagttcacc ttctggaaca gggattcgtt atgcggcagc
     1201 agggattcga tatctcgcag gatttcacgc gcctgctcca gttgcccacc cggtaaacgc
     1261 gctttacgca ccaggaagtt gagcgcgcgc tgggtatcca tcagacacag gcgaactttc
     1321 cagccgatat cttccagttc cgccagagtg gagagcgcct cgtcgtactc atcgccctga
     1381 tgcccttcca taatcacccg gctcaactgc tccaggtcgc tatagatatt ttcaatttca
     1441 tctgccaact gttcgatttt ggtttcgaac agatccagca gcagctcgta ggcgttaccg
     1501 tctaccatcg actggctacg ggcacgcata cgatacagac gaaaagcggg cagttcacgc
     1561 tcacgcagag taaacagacg accatcacgg atggtaaatg ccacagtgga gttaccggcg
     1621 tgatcttccg catcttcaaa gaagaagaag gagtgaatat gcaggccgtc gtcgtcttca
     1681 aagaaacgtg ccgatgcttc gatgtcttcc agttccgggc gggttgccag gctctggcca
     1741 agttcagatt gtacgcgcag tcgctcgtcg tcgtccggtt cgacaagatc aatccatact
     1801 gcatttacaa ggggttgtga ctcttcgact tccagccggg tcagtcggtt attttccagt
     1861 tgaaatgcgc tcagcatgac cgggactccc aatgcttaaa atatcggaca gttcggtggg
     1921 cacacagaaa caaattgggt ttcagaccat taaacagcct gactcagcgc gacgggaaaa
     1981 attgaggtcg ctgacaaccg ctaaggctat cagcaaaaag ggatagcctt aggagttgat
     2041 cctggatgac aggatagtga gccagtatct actgggtgtg tccaaggcga atgtcctctt
     2101 agagtgatcg tgcgcgcatg ttacgccagc aaaattttgc cgtcaacacg caacagaaca
     2161 ccacagaaca atagttgaac ttcgcgcttt ttggtactcg tcaaggtcgc taacaggata
     2221 aaaaaatcca acatccagga cctataatgg cgcatcggct actcattggt aaaggaatga
     2281 tcaccttgaa tctcaaacgg atttttctcg ccctcacctt acttccctta tttgctgttg
     2341 ccgctgatga ttgcgcactc tcagatccga tgctgaccgt acaggcgtat accgttaatc
     2401 ctcaaacaga gcgggtgaaa atgtactggc aaaaagccaa tggcgaagcg tggggaactt
     2461 tacatgctct gctggtggat atgaatagtc agggtcaggt gcagatggcg atgaacggcg
     2521 gcatctatga tgaaagctat gcgccgctcg gtttgtacat cgaaaacggt cagcaga
//
LOCUS       DADMKF010000292         2574 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.297, whole genome shotgun sequence.
ACCESSION   DADMKF010000292 DADMKF010000000
VERSION     DADMKF010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2574
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.297"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            1..146
                     /locus_tag="JNC24_004158"
                     /pseudo
     CDS             1..146
                     /locus_tag="JNC24_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001417502.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="extended Signal peptide of Type V secretion
                     system family protein"
     gene            143..2413
                     /locus_tag="JNC24_004159"
                     /pseudo
     CDS             143..2413
                     /locus_tag="JNC24_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001702658.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter barrel domain-containing
                     lipoprotein"
     gene            2415..2574
                     /locus_tag="JNC24_004160"
                     /pseudo
     CDS             2415..2574
                     /locus_tag="JNC24_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368382.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
BASE COUNT          699 a          535 c          729 g          611 t
ORIGIN      
        1 cattttagga tatcaagcga acagtaatgg taaggttatc gttgaaaagg gtggcgagtg
       61 gctaataaaa aataatgatt cctcaattga atttcaaatt ggtaatcaag gagctgggga
      121 ggcgactatt cgcgagggtg ggctgattac ggctgaaaat acgattatcg gtggcaatgc
      181 caccggtatc ggaaccctga atgtgcagga tcaagactct gtcatcacgg tacgcagact
      241 ctataatggg tatttcggta atggcacagt caatatttcc aataatggtc tgattaataa
      301 caaagaatat tcattggtgg gcgttcagga cggttcccac ggtgtcgtca acgtgaccga
      361 taaagggcat tggagtttcc tcggaacagg cgaagctttc cgctatatct atatcggtga
      421 tgctggtgac ggtgaactta atgtctcgcg tgaaggtaaa gtagattcgg gaattatcac
      481 tgcggggatg aaagaaacag gcacaggcaa cattactgtt aaggataaga actccgttat
      541 cactaatctc ggaactaatc ttggttatga cggccacggc gaaatgaata tcagtaatga
      601 ggggcttgtt gtcagcaacg gaggaagctc actcggttat ggagaaaacg gcgtcgggaa
      661 tgtcagcatc accacggggg ggatgtggga ggtcaataag aatgtctata ccaccattgg
      721 tgttgcgggc gtcggaaacc tcaatattag cgatggcggt aagtttgtat cgcaaaatat
      781 tacttttttg ggcgataaag caagcggtat cggcacactg aacctgatgg atgcgacatc
      841 atcgttcgat actgtgggta tcaatgtcgg taattttggt agcggtatcg taaatgtcag
      901 taatggtgcc acccttaatt caacgggcta tggatttatc ggaggaaatg cctccggtaa
      961 ggggatagtt aatatttcaa cggacagtct ctggaattta aagacatcat ctactaacgc
     1021 acaattgcta caggtcggtg tattaggcac gggtgaactg aatattacca ccggaggtat
     1081 agttaaagcg cgtgatacac agatagctct caatgacaaa agtaagggcg acgtgagggt
     1141 ggatgggcag aactctcttc ttgaaacatt caatatgtac gtagggacat ctggtacggg
     1201 tacgttaacc ctgacgaata gcggcacgct gaatgtcgaa ggtggagaag tttacttagg
     1261 tgtttttgaa cctgctgtag gaacgctaaa cattggtgca gctcacggtg aggcggcggc
     1321 agatgccggg tatataacca acgcgacgaa agtagagttt ggttctggcg aaggtgtttt
     1381 tgtctttaat catactaata acagtgatgc cggctaccag gtcgatatgc tgattacagg
     1441 tgacgataaa gacggaaaag tgatccatga tgcaggccat acggtgttca atgcagggaa
     1501 tacttatagc ggtaaaacgc tggtcaatga cggcctcctg accattgcgt ctcatacggc
     1561 agatggggta acgggcatgg gttcgagtga agtcaccatt gcaagccccg gtacgctcga
     1621 cattctcgca tcaacgaaca gtgcaggaga ttacacgctg accaatgcgc tcaaaggcga
     1681 tggcttgatg cgagtgcagc tgtcatccta cgacaagatg tttggcttta cccatgcaac
     1741 agggactgaa ttcgccggtg ttgcccaact gaaagacaga accttcactc tggaacgcga
     1801 caacaccgct gcgcttactc acgcgatgtt gcagtctgac agtgaaaata ccacatcggt
     1861 aaacgtggga gagcaatcca ttggtggact ggccatgaat ggcggtacgc tcattttcga
     1921 tacggatatt cctgctgcga cgcttgcaga gggatatatc agcgtcgata cgctggttgt
     1981 cggcgcgggt gactacacct ggaaaggccg taactatcag gtaaacggga cgggcgacgt
     2041 gcttatcgac gtgcctaaac cgtggaatga tcccatagcg aataaccctc tgacgacgct
     2101 caatttgctg gaacacgatg atagccatgt cggcgttcaa ctggtgaagg cgcaaacggt
     2161 tattgggtcg ggtggctcat taacgttacg tgatttacag ggcgacgagg tggaagcgga
     2221 caaaacgtta cacattgcgc aaaacggaac ggtggtcgcc gagggtgatt atggattccg
     2281 cctcacgacc gcaccaggtg atggtttgta cgttaactat gggctgaaag cgctgaacat
     2341 ccatggtggg caaaagctga cgttagccga acatggcgga gcctatggcg caacggccga
     2401 tatgtcggca aaatagccat cgcttacacg accgtctggg tgagccgcag tatacagatt
     2461 cactgcattc tcagggatcg gcaagcagta tgtggatgcg tcatgtcggg gggcacgaac
     2521 gtttcaggac cggtgacggc cagctaaata ctcaggctaa ccgctatgta ttgc
//
LOCUS       DADMKF010000293         2557 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.298, whole genome shotgun sequence.
ACCESSION   DADMKF010000293 DADMKF010000000
VERSION     DADMKF010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2557
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.298"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(59..262)
                     /gene="tatE"
                     /locus_tag="JNC24_004161"
     CDS             complement(59..262)
                     /gene="tatE"
                     /locus_tag="JNC24_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HAY9160755.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(391..1179)
                     /locus_tag="JNC24_004162"
     CDS             complement(391..1179)
                     /locus_tag="JNC24_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HAY9160756.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHVPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRRVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPVLNNRRFAPPQLL"
     gene            1272..1655
                     /gene="crcB"
                     /locus_tag="JNC24_004163"
     CDS             1272..1655
                     /gene="crcB"
                     /locus_tag="JNC24_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HAY9160757.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTAH"
     gene            complement(1709..1918)
                     /gene="cspE"
                     /locus_tag="JNC24_004164"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(1709..1918)
                     /gene="cspE"
                     /locus_tag="JNC24_004164"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002436076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HAY9160758.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(2093..>2557)
                     /gene="pagp"
                     /locus_tag="JNC24_004165"
     CDS             complement(2093..>2557)
                     /gene="pagp"
                     /locus_tag="JNC24_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HAY9160759.1"
                     /translation="TFRENIAQTWQQPEHYDLYIPAITWHARFAYDKEKTDRYNERPW
                     GGGFGQSRWDEKGNWHGLYAMAFKDSWNKWEPIAGYGWESTWRPLADENFHLGLGFTA
                     GVTARDNWNYIPLPVLLPLASVGYGPVTFQMTYIPGTYNNGNVYFAWMRFQF"
BASE COUNT          652 a          659 c          580 g          666 t
ORIGIN      
        1 ttttgaagcg gtttttttgt atcgaacaag atattgaggg agcgtcctgc tcgccacgtc
       61 actctttatg agagagcttt tcagcctgaa gatcaacgtc tgcgcctttt ttcgccgcag
      121 catcgtcatc attcatcgcc ttcttgaacc ctttaatggc cgctccaagg tctccgccca
      181 gcgtacgtaa cttcttagtc ccaaacagca gaacgaccag cgccgcaact accagcagtt
      241 tggtaatact aatctccccc atagatacct tcttgacata taaccggcaa cagaacgcgc
      301 cttaaaaacc agagaatatt aacggtcatt cgacgcgcgc acacaatagc aatctgtaac
      361 aaggtgaatc aagcgccgag taaaaaaaca tcataataat tgcggcggcg caaagcgacg
      421 gttgtttaag acgggcagtt gcgcgcgcac ctgacgcaca cgttcgggcg tcacttccgc
      481 cataatgagt gcaggcattt ctgacgctgc cgcaatggtg acgccaaagg gatctataat
      541 ccggctttgc ccgatatttt tgttcccgca ctcccccgcc gccaccatat aacaggtggt
      601 atccagcgca cgagcggcaa gcaacgttga ccagtgatgc tctttgagcg gcccgcgaac
      661 ccaggcggca ggaagtacca ggatttcagc tccctgtaat gcctgtgcca gcgccagctc
      721 tggaaagcgt aagtcataac aggtcatcag accgaccttc atcccctcca cctcgagtaa
      781 cggagcgatt tcattaccag catcaacacg gcgtgattcc tgaatggcaa atgcatcata
      841 gagatgcaat ttggcataac gggcgacgat gtttcctgcc tgtagcgcca ccagcatatt
      901 ccatgcgcgc cccggcgttg aaggaacatg aatcgtcaga attgtcgtca tcatgttacg
      961 tttactttct cgccgtaaac gtccgaggaa ttcgccttcc agcagctgtg ctgatttaac
     1021 cgatagatct gcatcatgat catcgcgcgc cagcaatgct tccggcagga cgaacagcga
     1081 tacgtcgttt tccgccgcct gcgccatcaa cgaggcacaa atctcagcgt tcttttccca
     1141 cacagatgta acagcaaact gtcctgcggc aaccagcatt gccacctcct gtcagagtcg
     1201 taaaattcgc caacgacgat tattcggcgt tacacttatc actcatacaa atcaaatagc
     1261 aggattttgc agtgttacaa cttcttttag cagtttttat tggcggtggt acgggaagcg
     1321 tggcgagatg gctgttaagt atgcgattta acccgctgca tcaggcgatt ccgttgggga
     1381 cgctggcagc aaacctgatt ggggcattca tcataggaat gggattcgcc tggttcagca
     1441 ggatgacgaa cattgatcca gtgtggaaag tattaatcac caccggattt tgtggcggtc
     1501 taacaacctt ctcaacattt tcggcagaag tggtgttttt gttacaagag ggccgctttg
     1561 gctgggcgtt actgaacgtt ttcgtcaacc ttctggggtc ttttgccatg accgcactgg
     1621 ctttctggct gttttcggcc tcaaccgcac actaaagacc aaaaaaaacc cgctgattaa
     1681 gcgggttttg aattcttgct gacgtatctt acagagcgat tacgtttgca gcagaagggc
     1741 ctttggcacc gttagtgatt tcgaactcta cgcgctgacc ttcagcaaga gttttaaaac
     1801 cattagtctg tattgcagag aagtgtacga acacgtcttt gctgccgtct tccggagtaa
     1861 tgaaaccgaa tcctttggac tcattaaacc acttaacgtt acctttaatc ttagacatca
     1921 aaattacctt tacatgaaaa atagacacaa atgctgtgtc ggttaccagt acaccaattg
     1981 tggtacgctt tgtccagtgc aactttacta aaaagtgata aaagtcgcgt tatttttgat
     2041 gttacttatt ttagttattg attttaaaga agttactaaa acttcatttg tctcaaaact
     2101 gaaagcgcat ccaggcaaag tacacattgc cattgttgta ggtacccgga atgtaggtca
     2161 tctgaaaagt cactgggcca taacccacgg aggccaatgg cagtagaacc gggagaggga
     2221 tgtaattcca gttatcgcgt gccgttacgc cagcggtgaa tcccagacct aaatgaaaat
     2281 tttcatccgc cagcggtcgc caggtacttt cccatccgta tccggcaatc ggttcccatt
     2341 tgttccacga gtccttaaat gccatggcat acaggccatg ccagtttcct ttttcatccc
     2401 atcgcgactg accaaaaccg ccaccccacg gtcgctcgtt atagcgatcg gttttttctt
     2461 tgtcgtaagc gaaacgtgca tgccaggtga tggcaggaat atataaatca taatgttcag
     2521 gctgttgcca ggtttgtgca atattttctc taaacgt
//
LOCUS       DADMKF010000294         2542 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.299, whole genome shotgun sequence.
ACCESSION   DADMKF010000294 DADMKF010000000
VERSION     DADMKF010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2542
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.299"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(108..1526)
                     /gene="phrB"
                     /locus_tag="JNC24_004166"
     CDS             complement(108..1526)
                     /gene="phrB"
                     /locus_tag="JNC24_004166"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HAY9160760.1"
                     /translation="MTTHLVWFRQDLRLHDNLALAAACRNSSARVLALYIATPRQWAT
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVEVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIEPSPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCQN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGVGSVWLNEL
                     IWREFYRHLITYHPSLCKHRPIIAWTDRVQWQSNPAHLQAWQEGKTGYPIVDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPTTQGEKFDLEGEFIRQWLPELRNVPGKSVHEPWKWAQKAGVKLDYPQP
                     IVEHKEARVQTLAAYEAARKGK"
     gene            complement(1523..2032)
                     /locus_tag="JNC24_004167"
     CDS             complement(1523..2032)
                     /locus_tag="JNC24_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HAY9160761.1"
                     /translation="MNLQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDYYNQYRQRVVVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYHDAWLSGQRYFELWPRV
                     INLRHSGVL"
BASE COUNT          642 a          660 c          580 g          660 t
ORIGIN      
        1 tcaggcctac gtatttcctg caatttattg aatttgcaca aatttgtagg ctggataagg
       61 cgttcacgcc gcatccggca tctgggctcg attgcctgac gcgtctgtta tttccccttc
      121 cgcgccgcct catacgctgc caacgtttgt actctcgcct ctttgtgctc gactatcggt
      181 tgcggataat ccagcttcac acctgctttc tgcgcccact tccacggctc atgcaccgat
      241 ttccccggta cattgcgtag ttccggtagc cactggcgga taaactcacc ttcgagatca
      301 aatttctcgc cctgggttgt cgggttgaaa atacgaaaat acggcgctgc atcggttccg
      361 gttgaagcgg cccactgcca gccaccgtta ttggctgcca aatcaccatc aatcagctgc
      421 gacatgaaat atcgctcgcc ttcgcgccag tcgatcaata aatctttcac cagaaaactg
      481 gctgtaatca tccgtagcct gttatgcatc cagccagtgc tgttaagctg acgcatagcg
      541 gcatcaacaa tcgggtatcc cgttttgcct tcctgccagg cctgtaaatg tgcgggattg
      601 ctctgccact gtacacgatc cgtccaggca ataattggac gatgtttaca caacgagggg
      661 tgatacgtta tcaggtgacg gtaaaactcg cgccagatca gctcattaag ccagacacta
      721 ccgaccccac cgtccagcgc ctgcggctgt tcagccaaca agcgatgcaa gcactggcga
      781 ggcgataacc cgcccgttgc caggctggcc gacaaacggc tggtgccttc cactgccgga
      841 aaatctcgtt gttgctcata ttctccggca ccgttctggc aaaactggcg taattgcgca
      901 atcgccgctt tttcttccac cggaaaatgc gcagtatcga aagactgacg aggataattc
      961 agcgtaatgg atggcgaggg ctctatcgat ccgctactac gaacttttgg cgcagcgacg
     1021 cactccggca tcccttcccg cagccgtttc agccaggcat tcttaaaagg cgtaaagact
     1081 ttgtacatct cgtgattacc ggtcatcacc gcgccaggcg gcaggatcac gctgtcatca
     1141 aatccttcac acaccacgtt acgcagcgct ctttcaactt ccacatcccg cgcccgctca
     1201 ttcacttcat actgatagtt ataaaacagg tgggtaacgc tgttttccgc gcacacctgt
     1261 ttaactattt cgacactggc gacaaagtca tccacttcac ggaacaataa aggaatacct
     1321 ttttccgcaa gcgctatttg tagcccattc agttgagcat tgataagttc agcctgacgc
     1381 ggcgacatgt tatgcgtcgc ccactggcgt ggtgtagcga tatacaacgc cagcacgcgt
     1441 gcagacgaat tgcggcaggc ggcagccagt gcgagattat cgtgcagacg taaatcttgg
     1501 cgaaaccaga ccagatgggt agtcataaaa ctcctgaatg gcgcaagtta atcacacgcg
     1561 gccaaagttc gaaataacgc tgccctgaaa gccaggcgtc atgataaagc gccatatagt
     1621 gtttgatacg catcagcggc gcaagaagtg gttgctcgcc acggcgataa ctgtcgataa
     1681 gcgcagccag ctgctggcgt tctgtggaat caatatgcgg gcgaaaataa ccctgaacgt
     1741 gcatcaaaac attggtgtga tcgcgcgggt tggcggggtg agaaagcaaa acaactacgc
     1801 gttggcggta ctggttgtaa tagtcatcca gattttgcca ctggtgaata tcagccacga
     1861 acgggccaag tttgcggtac tccggctgag aatgcgccag aaagacgagt ttatagcggc
     1921 tgtgataatc gagaagcgcc ccgcgcgtta agccatgttc tttcagtcga tgtaactcat
     1981 gaagggcaaa gatacggatt agggtgctgt catcaaatcg ttgtagattc attactgctc
     2041 ctgaacggta taaaggagta agtgtagacg atggtaaatg aattttatgg ggcagagttt
     2101 ttgcggggaa gtattttgtt ggtaaaaata ttatcaactt catctgagtg agaattattc
     2161 tactatttcc cgagctgcat tcattttttg tattttttat ttttagaaaa taacagttct
     2221 caaagcttaa ttacctcact attaattgta tttcaaacta acaatcgcac tccattttct
     2281 ctttttaaaa aacataaaca ctgttaaaac aaatacttat ttcgttatct taaatgctat
     2341 ttctacatca ctcgtcctta taatcaaatt attcttattc atagctattt ttgcaggttt
     2401 aaaatagcgg ctttcaccaa agtcagccat gaaaattaga ctacaagtca gggcaagatt
     2461 acgaaagccc gctccccgca aggactgacg ccagatagtt tctgtccatg gctgcttttc
     2521 gcatcttacg tcttaaccct gc
//
LOCUS       DADMKF010000295         2540 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.300, whole genome shotgun sequence.
ACCESSION   DADMKF010000295 DADMKF010000000
VERSION     DADMKF010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2540
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.300"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(14..88)
                     /locus_tag="JNC24_004168"
     tRNA            complement(14..88)
                     /locus_tag="JNC24_004168"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(54..56),aa:Gln,seq:ttg)
     gene            complement(112..196)
                     /locus_tag="JNC24_004169"
     tRNA            complement(112..196)
                     /locus_tag="JNC24_004169"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(160..162),aa:Leu,seq:tag)
     gene            complement(205..281)
                     /locus_tag="JNC24_004170"
     tRNA            complement(205..281)
                     /locus_tag="JNC24_004170"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(245..247),aa:Met,seq:cat)
     gene            complement(661..2325)
                     /gene="asnB"
                     /locus_tag="JNC24_004171"
     CDS             complement(661..2325)
                     /gene="asnB"
                     /locus_tag="JNC24_004171"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HAY9160762.1"
                     /translation="MCSIFGVFDIKTDAVEQRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKSLVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVHKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
BASE COUNT          610 a          638 c          628 g          664 t
ORIGIN      
        1 attgtttctt cgatggctgg ggtacctgga ttcgaaccag ggaatgccgg tatcaaaaac
       61 cggtgcctta ccgcttggcg ataccccatc cgtacaacgc tttctggtga atggtgcggg
      121 aggcgagact tgaactcgca caccttgcgg cgccagaacc taaatctggt gcgtctacca
      181 atttcgccac tcccgcaaaa aagatggtgg ctacgacggg attcgaacct gtgaccccat
      241 cattatgagt gatgtgctct aaccaactga gctacgtagc catctttttt tcgcgatacc
      301 ttatcggcgt tgcggggcgc attatgcgta tagagccttg cagcgtcaac ctctttttca
      361 aggaaaattg ctcgaaagtg actgtttggt taggttgcga acagcgtggc gctatattcg
      421 tcaattattg tttactttgt gttttttccc caccctacag ccattctttt gtcatacagg
      481 atgaaattcg gaattcaaca atggtggtgg gaaattaatc tatgaaatac tggcctacag
      541 tgatgagttg tcaaacagtg atgtggcaaa cccggaacat ttccttactg catatcagaa
      601 tcaacaagca cctcaataac tgaaacagcc ccggatttca ccggggctgt ttcgcatttc
      661 ttacttatac gccgactggt gaacgccaac cgcacgacca gacggatcgt ccattttctt
      721 gaacgcctca tcccactcaa tcgctttagc ggaagaacac gcgacggacg ggccgcccgg
      781 cacgcactca gcggcgctcg gaagcgggaa cagttcttca aagatctccc ggtacagata
      841 cgcttctttt gaggttggcg tgttgtacgg gaagcggaag cgggcagttt ccagttgctg
      901 atcagaaacc tgctgcgccg ccacttcttt cagggtgtcg atccaactgt aaccgacgcc
      961 atcggagaac tgctctttct gccgccaggc cacgcttgcg ggcaggtatg actcaaaaca
     1021 ttcacgcagg atgtgttttt ccattttgcc gttaccgcac attttatcct gcgggttaat
     1081 gcgcatcgcc acatcaagga attttttgtc gaggaacgga acgcgtgctt ccacgcccca
     1141 ggctgacatc gctttgttgg cgcgcgcgca gtcatacata tgcagggcca gcagtttatg
     1201 caccgtctct tcatgcagtt ctttagcgtt gggcgcttta tggaagtaaa gataaccgcc
     1261 aaacacttca tcagaacctt caccggacag caccatttta atgcccatcg ccttgatctt
     1321 acgcgacatt aaatacatcg gtgttgaagc gcgaatagtg gtcacatcat aagtttcgat
     1381 gtggtaaatc acgtcgcgga tggcatccag accttcctgt acagtgaagt gaatttcgtg
     1441 atgcaccgtg cccagatggt ttgccacttc ctgggctgct ttaagatccg gtgaacccgg
     1501 cagacctaca gcaaaggagt gtaactgggg ccaccaggct tcagagcgtt cctgatcttc
     1561 cacgcgacgg gctgcgtatt tcttggtgat agcggaaata attgaggaat ccagaccacc
     1621 agaaagcagc acaccgtaag gcacatcaga catcagatgg cttttaacgg aatcttccag
     1681 agcctgacgc agctcgtttt tgtcggtcac gttatctttc actgcatcaa agtcgaacca
     1741 gtcgcgatga taataagaac ggatttcgcc gtcctggctc cacaaatagc tccccgccgg
     1801 gaactcttta atcgtgcggc aaactggcac cagcgatttc atttctgagg ccacatacag
     1861 ctgaccgtgt tcgtcatacc ccatatacag tgggatgatc cccagatggt cgcgaccaat
     1921 caggtaggca tctttttcgc tgtcgtacag ggcaaaggca aacatgccct gcaagtcgtc
     1981 gagaaattcc ggcccttttt cctgatacag cgcgaggatc acttcacagt cagaccctgt
     2041 ctggaactga taacgatcgc catattcagc gcgcaatgcc tggtggttgt agatttcacc
     2101 gtttaccgcc agtacgtggg ttttttgttg gttgtagaga ggttgcgccc ccgcgttaac
     2161 gtcaacaatt gacagacgtt cgtgagcgag aatggcgtta tcgctggcat aaataccgga
     2221 ccagtccggg ccacgatgac gcatcaggcg tgacagctcg agtgctttct tacgctgctc
     2281 aactgcgtct gttttgatat cgaatacgcc aaaaattgaa cacataacct tctccgttaa
     2341 cctggtattt gttgcttgtt gtgtttgctt gtttaaaaaa atgccgcaaa gcagcactgt
     2401 gcgcaagcga tttggcggtg aaaaaataaa aaacgtaatg gtgattgtcg attggtgaaa
     2461 aagggtctgg tgtgaggatg tatttattga tgaatcgata atttttagcg ggttttattg
     2521 aatggttata ttttacgggg
//
LOCUS       DADMKF010000296         2472 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.301, whole genome shotgun sequence.
ACCESSION   DADMKF010000296 DADMKF010000000
VERSION     DADMKF010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2472
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.301"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(1..713)
                     /locus_tag="JNC24_004172"
                     /pseudo
     CDS             complement(1..713)
                     /locus_tag="JNC24_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001462196.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like adhesin"
     gene            complement(766..1362)
                     /locus_tag="JNC24_004173"
     CDS             complement(766..1362)
                     /locus_tag="JNC24_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY9160763.1"
                     /translation="MHPTQRKLMKRIILFLSLQFPITYPAIAGQDIDLVANVKNSTCK
                     SGISNQGNIDLGVVGVGYFSDNVTPESYQPGGKEFTVTVSDCALQGTGDVLNQLHIDF
                     RALSGVMAAGSRQIFANEVATGAKNVGVVLFSIQDPTNIFNVISSAGNSRSVYPVMTS
                     ALHNSSWKFYARMQKIDPALDVISGQVMSHILVDVYYE"
     gene            complement(1389..1991)
                     /gene="yadL"
                     /locus_tag="JNC24_004174"
     CDS             complement(1389..1991)
                     /gene="yadL"
                     /locus_tag="JNC24_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein YadL"
                     /protein_id="HAY9160764.1"
                     /translation="MTFKNLRYGLSSSVVLAASLFSVPSYAATDSIGLTVITTVEIGT
                     CTATLVNDSDQDISVVDFGDVYISEINAKTKVKTFKLKFKDCAGIPNKKAQIKLTKRA
                     TCEGTANDGAGLANGSTAADKASAVAVEVWSTETPATGSATQFSCVTPASQEVTISNA
                     ANAVVYYPMSARLVVEKNKTVSNVTAGKFSAPATFTVTYN"
     gene            complement(2006..>2472)
                     /locus_tag="JNC24_004175"
     CDS             complement(2006..>2472)
                     /locus_tag="JNC24_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY9160765.1"
                     /translation="ATTCNITLTGNNVTNDGNNNYTLRIPKMGLDKIANKTTESQADF
                     KLVASGCSSGISWIDTTLTGNASSSSPKLIIPQSGDSSSTTSNIGMGFKKRTTDDATF
                     LKPNSAEKIRWSTDEMQPDKGLEMTVALRETDAGQGVPGNFRALATFNFIYQ"
BASE COUNT          680 a          537 c          495 g          760 t
ORIGIN      
        1 tggcctgcgc cataggcttt aaatgaatac agataatttg atgagctgga taatttgatc
       61 tgttgagtta ctgttggatc aaagtttgta tctgtataga attcaattgt aagggtctga
      121 taaataccac ctacagccca gtatttacgg gtgctattcg cattattgca actccaatat
      181 aacacgtctt cgcttggatt ataccacgaa aaatattgtt ctgcagagtc accaatgtat
      241 atcccaggtg aactaacgtt agttacggta ccgtaagctg accacatgtt tgaaattaat
      301 aatgtgtaat acaaaccagg aactgatgta ttaaataatt tatgggagcc atacatttta
      361 cctgagtcaa ccattgcatt ttcaatacgc attgcgctac cacttgtgct atcacttctg
      421 ttgcaataaa gaaaaccacc accagaagca gtatctaact gactctactg taattttaca
      481 cctgtaggtc caagctgcgt cggtttgtag aaaactaaat tatttgtact tgttgcatat
      541 ctgaatatga cagtagcatc gccattcggc cccgtttctc tccatttttg atctgacgga
      601 ccaacgccgt aactattccc aacaatatat cctacgttat catcagtgcc agcactcgcg
      661 gtataacaac tcaagcacag aattaaaaaa aaatgtctga agaatttctt cattatcact
      721 ccaccatcta ctgctgtaac agtcgcagtt atttgttttg atctattatt cgtagtacac
      781 atcaactaat atatgactca ttacttggcc agatatgaca tccagcgcgg gatcgatttt
      841 ttgcattctg gcataaaatt tccatgaaga attatgcagg gcagaagtca ttactggata
      901 cacggaacga gaatttcctg ctgaactaat aacattgaat atattggtcg ggtcctgaat
      961 ggaaaagaga acgacaccga catttttagc cccggttgcg acttcattgg caaatatttg
     1021 ccttgaacct gcagccatga caccgctaag ggctctaaaa tcaatatgta actgattaag
     1081 cacatcgcca gttccctgta atgcacagtc tgatactgtg acagtgaact cttttccacc
     1141 tggttgatag ctttcaggag taacattatc tgaaaaatat cccaccccaa cgacgccaag
     1201 atcaatatta ccctggttac tgattccgct tttgcaagtg ctgtttttta cattggcaac
     1261 aaggtcaata tcctgcccag cgatggctgg ataggtaatg ggaaactgta gtgacagaaa
     1321 cagaattatt ctcttcatca gcttacgttg agtgggatgc atatatagtt cctggatctg
     1381 tcagtctgtt agttataggt tactgtaaat gtggcaggtg cagaaaattt acccgcagtg
     1441 acattgctta cggttttatt tttttcgacg accagtcgag cactcatcgg ataataaaca
     1501 accgcgttgg ctgcattgga gatagtgacc tcttgagatg ctggtgtcac acaactaaat
     1561 tgtgttgcgc tccctgttgc cggagtttcg gtgctccaga cttcaacggc gacggcactt
     1621 gctttatctg cggctgtgga tccatttgcg agccccgcac cgtcattagc agtaccttcg
     1681 catgtggctc gcttggttaa ttttatttgt gcttttttat tggggatacc cgcacagtct
     1741 ttgaatttga gtttgaatgt tttaactttg gtcttggcat tgatttcaga aatatataca
     1801 tcgccaaagt caacaacaga aatgtcctga tcagagtcat ttactaatgt agcggtacaa
     1861 gtacctattt ctacggtagt aatcacggtc agcccaatgc tgtctgttgc cgcataagag
     1921 ggtacgctga acaatgaggc agccaaaacg acactgctgg ataatccata acgtaaattt
     1981 ttaaatgtca tcttaattat ccttcttatt gatagatgaa attaaacgtt gccagtgcac
     2041 gaaaattccc cggtacgcct tgccctgcat ctgtttcacg tagcgcaacg gtcatttcaa
     2101 gacccttatc gggctgcatc tcgtctgtgc tccagcgtat cttttccgca ctgttaggtt
     2161 taaggaaagt ggcatcatca gtagtccgtt ttttgaaacc cataccgata ttacttgtcg
     2221 tcgaagatga atcaccagac tgcggtataa taagcttagg tgagcttgat gatgcatttc
     2281 cggtcagagt ggtatcaatc caactgatgc cactgctgca cccactagca accagtttaa
     2341 aatcagcctg agattctgtc gttttattcg cgatcttatc cagacccatc ttagggattc
     2401 tcaatgtgta gttattattg ccatcattcg tgacgttatt accagtaagt gtgatgttac
     2461 aggttgtcgc tt
//
LOCUS       DADMKF010000297         2462 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.302, whole genome shotgun sequence.
ACCESSION   DADMKF010000297 DADMKF010000000
VERSION     DADMKF010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2462
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.302"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            <1..>2462
                     /locus_tag="JNC24_004176"
     rRNA            <1..>2462
                     /locus_tag="JNC24_004176"
                     /product="23S ribosomal RNA"
BASE COUNT          635 a          540 c          783 g          504 t
ORIGIN      
        1 tcctgactga ccgatagtga accagtaccg tgagggaaag gcgaaaagaa ccccggcgag
       61 gggagtgaaa aagaacctga aaccgtgtac gtacaagcag tgggagcacg cttaggcgtg
      121 tgactgcgta ccttttgtat aatgggtcag cgacttatat tctgtagcaa ggttaaccga
      181 ataggggagc cgaagggaaa ccgagtctta actgggcgtt aagttgcagg gtatagaccc
      241 gaaacccggt gatctagcca tgggcaggtt gaaggttggg taacactaac tggaggaccg
      301 aaccgactaa tgttgaaaaa ttagcggatg acttgtggct gggggtgaaa ggccaatcaa
      361 accgggagat agctggttct ccccgaaagc tatttaggta gcgcctcgtg aattcatctc
      421 cgggggtaga gcactgtttc ggcaaggggg tcatcccgac ttaccgaccc gatgcaaact
      481 gcgaataccg gagaatgtta tcacgggaga cacacggcgg gtgctaacgt ccgtcgtgaa
      541 gagggaaaca acccagaccg ccagctaagg tcccaaagtc atggttaagt gggaaacgat
      601 gtgggaaggc ccagacagcc aggatgttgg cttagaagca gccatcattt aaagaaagcg
      661 taatagctca ctggtcgagt cggcctgcgc ggaagatgta acggggctaa accatgcacc
      721 gaagctgcgg cagcgacgct tatgcgttgt tgggtagggg agcgttctgt aagcctgcga
      781 aggtgtgctg tgaggcatgc tggaggtatc agaagtgcga atgctgacat aagtaacgat
      841 aaagcgggtg aaaagcccgc tcgccggaag accaagggtt cctgtccaac gttaatcggg
      901 gcagggtgag tcgaccccta aggcgaggcc gaaaggcgta gtcgatggga aacaggttaa
      961 tattcctgta cttggtgtta ctgcgaaggg gggacggaga aggctatgtt ggccgggcga
     1021 cggttgtccc ggtttaagcg tgtaggctgg ttttccaggc aaatccggaa aatcaaggct
     1081 gaggcgtgat gacgaggcac tacggtgctg aagcaacaaa tgccctgctt ccaggaaaag
     1141 cctctaagca tcaggtaaca tcaaatcgta ccccaaaccg acacaggtgg tcaggtagag
     1201 aataccaagg cgcttgagag aactcgggtg aaggaactag gcaaaatggt gccgtaactt
     1261 cgggagaagg cacgctgata tgtaggtgaa gtcccttgcg gatggagctg aaatcagtcg
     1321 aagataccag ctggctgcaa ctgtttatta aaaacacagc actgtgcaaa cacgaaagtg
     1381 gacgtatacg gtgtgacgcc tgcccggtgc cggaaggtta attgatgggg tcagcgcaag
     1441 cgaagctctt gatcgaagcc ccggtaaacg gcggccgtaa ctataacggt cctaaggtag
     1501 cgaaattcct tgtcgggtaa gttccgacct gcacgaatgg cgtaatgatg gccaggctgt
     1561 ctccacccga gactcagtga aattgaactc gctgtgaaga tgcagtgtac ccgcggcaag
     1621 acggaaagac cccgtgaacc tttactatag cttgacactg aacattgagc cttgatgtgt
     1681 aggataggtg ggaggctttg aagtgtggac gccagtctgc atggagccgg ccttgaaata
     1741 ccacccttta atgtttgatg ttctaacgtg gacccgtgat ccgggttgcg gacagtgtct
     1801 ggtgggtagt ttgactgggg cggtctcctc ctaaagagta acggaggagc acgaaggttg
     1861 gctaatcctg gtcggacatc aggaggttag tgcaatggca taagccagct tgactgcgag
     1921 cgtgacggcg cgagcaggtg cgaaagcagg tcatagtgat ccggtggttc tgaatggaag
     1981 ggccatcgct caacggataa aaggtactcc ggggataaca ggctgatacc gcccaagagt
     2041 tcatatcgac ggcggtgttt ggcacctcga tgtcggctca tcacatcctg gggctgaagt
     2101 aggtcccaag ggtatggctg ttcgccattt aaagtggtac gcgagctggg tttagaacgt
     2161 cgtgagacag ttcggtccct atctgccgtg ggcgctggag aactgagggg ggctgctcct
     2221 agtacgagag gaccggagtg gacgcatcac tggtgttcgg gttgtcatgc caatggcact
     2281 gcccggtagc taaatgcgga agagataagt gctgaaagca tctaagcacg aaacttgccc
     2341 cgagatgagt tctccctgac cctttaaggg tcctgaagga acgttgaaga cgacgacgtt
     2401 gataggccgg gtgtgtaagc gcagcgatgc gttgagctaa ccggtactaa tgaaccgtga
     2461 gg
//
LOCUS       DADMKF010000298         2446 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.303, whole genome shotgun sequence.
ACCESSION   DADMKF010000298 DADMKF010000000
VERSION     DADMKF010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2446
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.303"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(25..315)
                     /locus_tag="JNC24_004177"
     CDS             complement(25..315)
                     /locus_tag="JNC24_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HAY9160766.1"
                     /translation="MLGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(305..781)
                     /gene="ratA"
                     /locus_tag="JNC24_004178"
     CDS             complement(305..781)
                     /gene="ratA"
                     /locus_tag="JNC24_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HAY9160767.1"
                     /translation="MILFVGFLLMEIVMPQISRTALVPYSAEQMYQLVNDVQSYPQFL
                     PGCTGSRILESTPGQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGG
                     WKFTPLSQEACRIEFHLDFEFTNKLIELAFGRVFKELASNMVQAFTVRAKEVYSAR"
     gene            913..1395
                     /gene="smpB"
                     /locus_tag="JNC24_004179"
     CDS             913..1395
                     /gene="smpB"
                     /locus_tag="JNC24_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HAY9160768.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            1610..1972
                     /gene="ssrA"
                     /locus_tag="JNC24_004180"
     tmRNA           1610..1972
                     /gene="ssrA"
                     /locus_tag="JNC24_004180"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(2171..>2446)
                     /locus_tag="JNC24_004181"
     CDS             complement(2171..>2446)
                     /locus_tag="JNC24_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HAY9160769.1"
                     /translation="GRIWNAGPDSLGRLSPVVMLAKSVTAQTHMAWGDADNQQVKLSM
                     PELEELAAAMVQAQVDRNDEIYRRQREMKEELSGLEDLGTIRAFDVT"
BASE COUNT          667 a          655 c          549 g          575 t
ORIGIN      
        1 caatgagcac cttttttctg tctgttattt attcgctgat ttttctgctc gttgcctgcg
       61 aagctctttc ggatcggcaa tgagagggcg ataaatctcc acccgatcgc catcatgcac
      121 actatcgctt agttttgccg gacggctgta aatgccgact ttgtttttag ttaaatcgat
      181 atcggtacgc aattccagca agccactggc gcgaatagct tcttcaaccg tcgcgccctc
      241 ctgcagcgtc acgcgctgca ggtactgctt ctcaggtagc gcataagcca cctcaacggc
      301 aattttacct agcactgtaa acctctttcg cacgaaccgt aaaagcctgg accatattag
      361 acgccagctc tttaaacacg cgaccaaagg cgagttcaat caacttattg gtaaactcaa
      421 agtcgagatg aaactcgata cgacacgcct cctggctcag cggcgtaaac ttccatccac
      481 caatcaattt cttgaacggc ccatccacca gattcataag aatactttgg ttactggtca
      541 actggttgcg ggtagtaaac gttttgctga tcccagcctt agagacatct accgccgcag
      601 tcatctgccc aggagtggac tccagaatcc gacttccggt acaacccggc aaaaactgag
      661 gataagactg aacgtcattc actaactgat acatttgctc cgcgctgtag ggtaccagtg
      721 cggtccggct aatctgaggc ataactattt ccatcaacaa aaatccaaca aataatatca
      781 tttatcctgc tgtaaaaaaa acgctatccc ggcgctgggt aacatcgggt tcatgctaag
      841 atagagcctt gtcccccgca ggattgatat ggggtgtttt cgatttcaga ttaccgatga
      901 ttcacgacgc ttatgacgaa gaaaaaagca cataaacctg gttcagcgac catcgcgctt
      961 aacaagcgcg cccgtcacga atactttatc gaagaagagt tcgaagcggg acttgccctg
     1021 caaggctggg aagttaaatc cctgcgcgca ggaaaagcca atatcagcga cagctacgtc
     1081 cttctgcgtg acggagaggc atttctgttt ggcgctaaca tcacgccaat ggccgtggcc
     1141 tccacgcatg tggtgtgcga tcctacccgt acccgcaagt tacttctcaa ccagcgcgaa
     1201 ctggactcat tgtacggtcg cgtcaatcga gaaggctata ccgtagtggc gctctccctg
     1261 tactggaaaa atgcctggtg caaagtgaaa atcggcgtcg ccaaaggtaa aaaacagcac
     1321 gataaacgtt cagatatcaa agaacgcgaa tggcaggtgg ataaagcacg tatcatgaaa
     1381 aacgcccacc gttaaacctg cactccaatt attgaccagt tcctcaccgc gcctccctct
     1441 ccggcggcgc gaatgaacat cttattggct atcacatccg acacaaatgt tgccatccca
     1501 ttgcttaatc gaataaaaat caggctacat gggtgctaaa tctttaacga taacgccatt
     1561 gaggctggtc atggcgctca taaatctggt atacttacct ttacacattg gggctgattc
     1621 tggattcgac gggatttgcg aaacccaagg tgcatgccga ggggcggttg gcctcgtaaa
     1681 aagccgcaaa aaatagtcgc aaacgacgaa aactacgctt tagcagctta ataacctgct
     1741 tagagccctc tctccctagc ctccgctctt aggacgggga tcaagagagg tcaaacccaa
     1801 aagagatcgc gtggaagccc tgcctggggt tgaagcgtta aaacttaatc aggctagttt
     1861 gttagtggcg tgtccgtccg cagctggcaa gcgaatgtaa agactgacta agcatgtagt
     1921 accgaggatg taggaatttc ggacgcgggt tcaactcccg ccagctccac cacttttgac
     1981 aggactgcaa ccggacagag actataaaaa cagtcactta cggacactga ccagacagta
     2041 agcggaccag aaaaagacaa aaatatacac gcaaaatgca cgtgcatttc aaaaaagcct
     2101 ctgattatga agtcagaggc ttttctagtt gtaaataagg gtaacaattt aacacgatgc
     2161 agcttaccaa ttacgtaaca tcaaacgctc taattgttcc caaatcctcc agaccactca
     2221 gctcctcttt catttcacgc tgacggcgat aaatctcatc gttgcgatcg acctgcgcct
     2281 gcaccattgc tgccgccagt tcttccagtt ccggcatcga cagtttcacc tgctgattat
     2341 cggcatcacc ccacgccata tgcgtttgtg ctgtgacaga ttttgccagc atgactaccg
     2401 gggacaggcg gcccagtgag tcggggccag cattccagat acgacc
//
LOCUS       DADMKF010000299         2438 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.304, whole genome shotgun sequence.
ACCESSION   DADMKF010000299 DADMKF010000000
VERSION     DADMKF010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2438
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.304"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(443..519)
                     /locus_tag="JNC24_004182"
     tRNA            complement(443..519)
                     /locus_tag="JNC24_004182"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(483..485),aa:Arg,seq:tct)
     gene            complement(533..609)
                     /locus_tag="JNC24_004183"
     tRNA            complement(533..609)
                     /locus_tag="JNC24_004183"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(573..575),aa:Arg,seq:tcg)
     gene            complement(617..692)
                     /locus_tag="JNC24_004184"
     tRNA            complement(617..692)
                     /locus_tag="JNC24_004184"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(657..659),aa:Ile,seq:cat)
     gene            complement(733..1791)
                     /locus_tag="JNC24_004185"
     CDS             complement(733..1791)
                     /locus_tag="JNC24_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HAY9160770.1"
                     /translation="MLNTVKISSCELINADCLEFIRSLPENSVDLIVTDPPYFKVKPE
                     GWDNQWKGDDDYLKWLDQCLAQFWRVLKPAGSLYLFCGHRLASDIEIMMRERFSVLNH
                     IIWAKPSGRWNGCNKESLRAYFPATERILFAEHYQGPYRPKDAGYAAKGSALKQHVMA
                     PLISYFRDARAALGITAKQIADATGKKNMVSHWFSAGQWQLPNESDYLKLQALFARVA
                     EEKHRRGELEKLHHQLVDTYTSLNRQYAELLSEYKHLRRYFGVTVQVPYTDVWTHKPV
                     QFYPGKHPCEKPAEMLQQIISASSRPGDLIADFFMGLGSTVKAALALGRRAIGVELET
                     ERFEQTVREVQDLVSQNG"
     gene            complement(1899..2139)
                     /locus_tag="JNC24_004186"
                     /pseudo
     CDS             complement(1899..2139)
                     /locus_tag="JNC24_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309113.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2364..>2438)
                     /locus_tag="JNC24_004187"
     CDS             complement(2364..>2438)
                     /locus_tag="JNC24_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HAY9160771.1"
                     /translation="YDALVTRFDIEEAWAERQLKKVTR"
BASE COUNT          586 a          698 c          560 g          594 t
ORIGIN      
        1 atagcggtca tgtgatgccg gtttcccggt aactcagcac cggtatctga gtcaacgttt
       61 tctctactgg gtcatttccg atacgccctg cctgctgaca ggctttcatc acatctgaaa
      121 atatagcacc ctgactgata ctgtagtacc taaggttcca gaaactgtga tgtatccggc
      181 acagaaaagc ccctccggag agggctggag agtggcgcta tgtgccattg catgatgccg
      241 ggtgcctccc ggtgaattca gtaccagcac ctgaatccgc gattatccca tatacctact
      301 cgctgattgc ccctccgcac agggggattc accatgccag tttcttttaa caaactcccc
      361 gcaaaacaga caactgtcaa ccgtctgaat tgtgagacat ttaaaaaaaa ggcccgcaaa
      421 agcgagccag ggaaaataag tgtggcgcgt tgtactggat tcgaaccagt gaccgattgc
      481 ttagaaggca attgctctgt ccggctgagc taacaacgca tgatgcagat aatggaccgc
      541 catcggggac ttgaaccccg cgcagccagc ttcgaaggct ggcgctcttt cctgatgagc
      601 taatggcggt atgtgatggt ggcccttgct ggatttgaac cagcgacctg gcgattatga
      661 gtcgctcgct ctcaccactg agctaaaggg ccgggagccg cataataacg acgcgtaatt
      721 aattcttcaa tatcatccgt tctggctgac taaatcctgt acttcccgaa ccgtctgctc
      781 aaaacgttca gtctccagct caacgccaat tgcacgacgc ccgagcgcca gtgccgcttt
      841 cactgtcgaa cccaacccca tgaaaaaatc tgcaatcagg tcacccggac gactgctagc
      901 gctgattatc tgctgcagca tttctgccgg tttttcgcac ggatgtttcc cgggatagaa
      961 ctgcaccggt ttatgcgtcc acacatcggt atacggcacc tgcaccgtca cgccaaaata
     1021 ccgccgcaga tgtttatatt cactcagcag ctccgcatac tgccggttca gtgaggtata
     1081 cgtatccacc agctggtggt ggagcttttc cagttcacca cgccgatgct tctcttctgc
     1141 cacccgggca aacagcgcct gtaatttcag ataatcgctt tcattcggca actgccactg
     1201 accggcactg aaccagtgcg acaccatgtt tttctttcct gtggcatctg caatctgttt
     1261 tgccgttatc cccagggcag ctcgcgcatc acgaaagtaa gaaatcagcg gggccatcac
     1321 atgctgtttc agtgcactgc ccttcgccgc atacccggca tctttcggac gatacggccc
     1381 ctgataatgt tccgcgaaca gaatgcgctc tgtggcgggg aaatacgccc gcaggctttc
     1441 cttgttgcat ccgttccagc gtccggacgg cttcgcccag ataatatggt tcagcacact
     1501 gaagcgttca cgcatcatga tttcgatatc agatgccagg cgatgaccac agaacaggta
     1561 aagacttccg gcaggtttca gcacccgcca gaactgcgcc agacactggt ccagccactt
     1621 caggtaatca tcgtcgccct tccactggtt atcccagccc tcaggcttca ctttaaagta
     1681 cggcgggtcc gtgactatca ggtcaacaga attttcgggt aacgaccgga taaattccag
     1741 gcagtcggcg ttgattaact cacaactgga tatttttaca gtattaagca tggatcatta
     1801 agcctgtctc tgataggctc attctgcttt tgcgcaaagc agtgggcctg aggtttgctt
     1861 gtgaacccaa cgcatgagca gatggctggt gggtgcccct aacacccacc agccgcccat
     1921 ttaccacaaa taaaaaagcc ttcactgcgg aaggcgtctg taacaaccga actgatagtc
     1981 tgccagaccc gccataacca gctgggtcag tattaactgg cagcgttcgc gtgaaaggta
     2041 agtattctgc gctatctccc cgactgtcgc cggttcggta acgcttaatt cattaaacac
     2101 cactctggcg gtttctgtca tatcctgctg ttttagcatg tcttttcctt ttccggttaa
     2161 cgtgacacac caataactct tgtcgaaaaa gccagcaagc tgaaagacag gtattcaccg
     2221 ccaccagcgc gtttactgta ctgacgcgat ttcagtcata aaaaacccgc tcggcggcgg
     2281 gttgtagaaa ctcttctaac gtcaggcata aaacgcccat cgttatgacg aatttaccac
     2341 agattccgga aaaatcaacc ttgttaccta gttacctttt ttaactgccg ctcagcccat
     2401 gcttcttcaa tatcaaaccg ggtcaccagc gcatcata
//
LOCUS       DADMKF010000300         2403 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.305, whole genome shotgun sequence.
ACCESSION   DADMKF010000300 DADMKF010000000
VERSION     DADMKF010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2403
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.305"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            375..641
                     /gene="hipB"
                     /locus_tag="JNC24_004188"
     CDS             375..641
                     /gene="hipB"
                     /locus_tag="JNC24_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HipB"
                     /protein_id="HAY9160772.1"
                     /translation="MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISN
                     FENNPDNTTLTTFFKILQSLELSMTLCDAKNASPEATEQQDLEW"
     gene            641..1962
                     /gene="hipA"
                     /locus_tag="JNC24_004189"
                     /pseudo
     CDS             641..1962
                     /gene="hipA"
                     /locus_tag="JNC24_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416024.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system serine/threonine
                     protein kinase toxin HipA"
     gene            2146..2247
                     /locus_tag="JNC24_004190"
                     /pseudo
     CDS             2146..2247
                     /locus_tag="JNC24_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310140.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
BASE COUNT          667 a          504 c          618 g          614 t
ORIGIN      
        1 agatttggcg cagtggagta gcaacgcgca ggatcgctgg catcttggcg tgatggcagg
       61 ctacgccaat cagcattagc ctgtggggga atgtcggtgt gcaactaggt gataaaggct
      121 atagcgatac tcagggcatg ctgggagtga aatatagctg gtaaaccgta taagccgcat
      181 gtcgagatgg catgcggctt aatattgcca acttcaaaag gcttttaaag cctccctttt
      241 atccgcgatc gcggatatcg cagcgtttat cccgtacagc ggataagatg tgtttccaga
      301 ttgactttat cctcactaaa ggataaaact gataatatcc ccttaagcgg ataaatttgc
      361 tgtggacgta tgacatgatg agctttcaga agatctatag cccaacgcaa ttggcgaatg
      421 caatgaaact ggttcgccag caaaatggct ggacgcagag cgagctggcg aaaaaaattg
      481 gcattaagca ggcgacgatt tccaatttcg aaaacaaccc tgacaatacc acgctgacga
      541 ccttttttaa gattttacag tcgcttgaac tctcaatgac gctatgcgac gcgaaaaatg
      601 cctcgccaga agcaacagaa cagcaagatc tggagtggta atgcctaaac ttgtcacttg
      661 gatgaacaac cagcgggtag gcgagttaac gaagttagcc aacggcgcgc acacctttaa
      721 gtatgcaccg gagtggttag ccagccgtta tgccagaccg ttgtcacttt cgctgccatt
      781 gcagaggggg aatatcacct ctgatgccgt atttaacttc ttcgataacc tgttacccga
      841 tagcccgatt gtacgtgacc ggatcgttaa acgttatcat gccaaatcca gacaaccgtt
      901 tgatttgttg tcagaaatag ggcgagacag cgttggtgcc gtgacgttaa tacccgaaga
      961 cgaaaccgta acgcgtccga taatggcatg ggaaaagctt actgaagcca gacttgaaga
     1021 agtattaacg gcttataaag cagatatccc gctaggcatg attagagaag aaaatgactt
     1081 tcgcatctcg gttgctggcg cacaggagaa gacagcactg ctcagaatag gcaatgactg
     1141 gtgcattccg aaaggaataa cgccgacgac gcacatcatt aaattaccga ttggcgaaat
     1201 caggcagtcc aatgcgacgc tcgatctcag ccaaagcgtt gataatgagt attactgtct
     1261 gctgctggcg aaagaacttg ggttgaatgt tccggacgca gaaatcatta aagcgggaag
     1321 ggtgcgcgcg ttagcggtcg aacgttttga caggcgttgg aatactgagc gaacggtttt
     1381 acttcgcttg ccacaggagg atatgtgtca gacattcggt ttaccttcat cggtgaaata
     1441 tgaatcagat ggaggcccag gcatcgcgcg gatcatggcg tttttgatgg ggtccagcga
     1501 ggcgctgaaa gatcgctatg attttatgaa gtttcaagtg ttccagtggt tgattggcgc
     1561 aacggatggc catgcaaaaa acttctccgt atttattcag gctggcggca gttatcgact
     1621 cacgccattt tacgacatca tttcagcatt tccggtcctt ggcggtacgg gaataacatc
     1681 agcgatctca aactggcaat ggggcttaac gcatccaaag gcaaaaaaac ggcaatcgat
     1741 aaaatttatc cgcgacattt tttggcgaca gcaaaggtgc tgagattccc ggaagtgcag
     1801 atgcatgaaa tcctaagtga ctttgccaga atgattccgg cagcactgga taacgtgaag
     1861 acttcattac cgacagattt tccggagaac gtggtgacgg cagttgaaac caatgtgttg
     1921 aggttgcatg gacggttaag ccgagaatac ggtagtaagt gagatatggg gtttggtcgt
     1981 tgttaatgag caatcatgac cggccaacga ctggattctc ccttggttta tgtttgtgcg
     2041 cataatgtat attatgttaa atcatgtctg agtgatgcga agtgcttttc tggtagtcgt
     2101 tattcgttca gattaggtgt ccttgtgatt taaagatgtt atcgtggcac gaatatactg
     2161 ccattccagg ctcgttatat tgctctcggg cagtccgtag ctggtactgc aaacgctgat
     2221 gcgaccttca aagttcaata tctataattc aatcttaagg tggctatacg tcatctgaaa
     2281 ttgaaacagg gtgtgtgtgg catggctacg cacacccgag caattcacca atgtgaatct
     2341 cattattaat gaggagtggt ttgtatttgg agcgctatga aaaatcacat aatatctaaa
     2401 cta
//
LOCUS       DADMKF010000301         2379 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.306, whole genome shotgun sequence.
ACCESSION   DADMKF010000301 DADMKF010000000
VERSION     DADMKF010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2379
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.306"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(561..740)
                     /locus_tag="JNC24_004191"
     CDS             complement(561..740)
                     /locus_tag="JNC24_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001485364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Par-like protein"
                     /protein_id="HAY9160773.1"
                     /translation="MSISFFEENLKDIDALITAEMVSGKGRVNRSDVVRAAVEALKTL
                     SKTEVSRLIAEVKQR"
     gene            complement(860..1486)
                     /locus_tag="JNC24_004192"
     CDS             complement(860..1486)
                     /locus_tag="JNC24_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010260829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family plasmid-partitioning AAA ATPase"
                     /protein_id="HAY9160774.1"
                     /translation="MIIVLGSQKGGVGKSTLAVSIAAYLMSLGNRVIIVDADDQKSVL
                     TWYNNRPEDLPHIPVTGATGNIKAMLKEHEKSYDFVIADCAGRDSAEMRSGLMAADVF
                     ISPLRPSQMDLDVVPHTCSVFTAAKDFNEDVRGYLVLNMTPTNMFVNEANEAAEVLKD
                     YPEMHLANSRVCDRKAHRDAWAESMTIFETQNDKAQQEIEALVKEVIL"
     gene            complement(1690..>2379)
                     /gene="umuC"
                     /locus_tag="JNC24_004193"
     CDS             complement(1690..>2379)
                     /gene="umuC"
                     /locus_tag="JNC24_004193"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V subunit
                     UmuC"
                     /protein_id="HAY9160775.1"
                     /translation="KLNALGINTALQLAQANTAFIRKNFSVILERTVRELNGESCISL
                     EEAPPAKQQIVCSRSFGERITDKDAMHQAVVQYAERAAEKLRGERQYCRQVTTFVRTS
                     PFAVKEPCYSNAAVEKLSLPTQDSRDIIAAACRALNHVWREGYRYMKAGVMLADFTPS
                     GIAQPGLFDEIQPRKNSEKLMKTLDELNQSGKGKVWFAGRGTAPEWQMKREMLSPAYT
                     TRWTDLPVAQL"
BASE COUNT          621 a          546 c          487 g          725 t
ORIGIN      
        1 tttctggtgc tccaggttgt gtgacatggg aactcattct ggatggttac tctgaaagct
       61 catattctgc cacaccccga tttgcagccg ccaggctgcc gtggttcatc tctgcctttg
      121 tttcatgggg tgttctttgt atattttact tactggggtc cgcttggaat tcggaaaata
      181 aaccacgtta agactatttt ttagtcaaag gactttcagg aatcgccaga atggcgggac
      241 aacagcggtt ttggtgtccg aaatcgtaca attctgtaca tctacgcaag ggaatgtacg
      301 gaagtaagcc aaacggttat cggacggccg gaacatttcg tacagccttt acgttcagtg
      361 agttagctgg cctggatgta accactcgtt caactcaatt ctcttagcgt gggaaaattt
      421 ccgaattcca agcggacccc tattaatatt tgttttgttt taggttctat tcaacatagg
      481 tcatcctaaa atacattgaa gataacaaga taacaagata acaagataac aagataacaa
      541 gataacaaga taacaaggtg ttatctttgt tttacttctg ctatcaatcg tgatacctct
      601 gtttttgata gtgttttaag tgcttctacc gccgccctga caacatcaga acgattcact
      661 ctgcctttac ctgaaaccat ctcagccgtt ataagggcat caatgtcttt taggttttct
      721 tcaaaaaaag aaatactcac tggttttgct ttgcttacaa ccgatgattt atctgatgac
      781 tttttggtat tatcaaggcc agccgtgcca ccgttaataa atgctgcctc gtcacgaggt
      841 gaagtgtttg ctctatttct cacagaataa cctctttcac taacgcttcg atttcttgct
      901 gggctttatc attttgcgtt tcaaaaattg tcattgattc ggcccaagca tctcgatgtg
      961 ctttacgatc acatacccgg gagtttgcca gatgcatttc agggtaatcc ttaagtactt
     1021 cagctgcctc attggcttca ttaacaaaca tatttgtggg cgtcatgttt aaaacgagat
     1081 atccccgaac atcctcatta aaatcttttg ctgctgtaaa aacggagcat gtatgaggca
     1141 caacatccag gtccatctga gatgggcgca aaggagaaat gaatacatct gcggccatca
     1201 atccactacg catttctgcg ctatcacggc cagcacagtc tgcaataaca aaatcatagg
     1261 atttttcgtg ttcctttaac atggctttga tgttacctgt cgcgcctgta accggaatat
     1321 gagggagatc ttcagggcga ttgttgtacc aggttaatac tgatttctgg tcatctgcgt
     1381 ctacaataat cactctattg cctagtgaca tcagatacgc agctatagat accgccagtg
     1441 tacttttacc taccccaccc ttttgactac ctaatactat tatcattata gccccatatt
     1501 aagttgttaa gttaacaact taatattgcc atctgaacgc gaatcatgca attatttttg
     1561 ttatattaac aacttaataa gttgttaagt atttgattta tagattaatt aagataacaa
     1621 gataacaaga taacaagata acaagataac aagataacaa gataacaata ttatgggtgg
     1681 attacatatt cataactggg ctactggcaa atctgtccat ctggtggtat acgcaggaga
     1741 aagcatttcc cgtttcattt gccactcagg ggcggttcct cgccccgcaa accacacttt
     1801 cccttttccc gactggttca gttcatcgag tgttttcatt aacttttcac tgtttttacg
     1861 gggctggatt tcatcaaata atcccggctg tgctataccc gatggtgtga aatcagccag
     1921 catgactcct gccttcatat agcggtaccc ttcacgccag acatggttta aggccctgca
     1981 tgcggcggca ataatgtccc ggctgtcctg tgtgggcaat gaaagctttt ccacagcggc
     2041 attgctgtaa cagggttctt ttactgcaaa gggggatgtc cgtacaaatg tcgtcacctg
     2101 ccggcaatac tgacgttccc cacgtagttt ctctgcggcc cgctctgcat actgaacaac
     2161 agcctggtgc atggcatctt tgtctgtgat tcgttcacca aaactgcgac tacagacaat
     2221 ctgctgtttt gccggcggtg cttcttccag agatatgcag gactcgccgt tgagttcgcg
     2281 taccgtacgc tcaagaatga cgctgaagtt tttccggatg aatgccgtgt tagcctgcgc
     2341 cagctgcagt gctgtgttaa tacccagcgc attcagctt
//
LOCUS       DADMKF010000302         2329 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.307, whole genome shotgun sequence.
ACCESSION   DADMKF010000302 DADMKF010000000
VERSION     DADMKF010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2329
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.307"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            20..238
                     /locus_tag="JNC24_004194"
     CDS             20..238
                     /locus_tag="JNC24_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 domain-containing protein"
                     /protein_id="HAY9160776.1"
                     /translation="MVTLFRKKYPRKSRTTEFLFLILFIVLMTPISPLIFVWIIGKII
                     EPVIELYNDVVWASFNTLHNKINPYKES"
     gene            240..605
                     /locus_tag="JNC24_004195"
     CDS             240..605
                     /locus_tag="JNC24_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAY9160777.1"
                     /translation="MALTKKQRAELRMKFGGRCAYCGCELGEKWHADHVKPVIRFDGN
                     MLHQERDDISNMVPACHPCNLHKHCSSLEDYRRIISDGRREFLASGKGKALVRMGLVE
                     MKSDPVVFWFEKYQEGATA"
     gene            602..1267
                     /locus_tag="JNC24_004196"
     CDS             602..1267
                     /locus_tag="JNC24_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9160778.1"
                     /translation="MTTITKERLQWLANISGRDDIDDIDGGEIRELALIALASLEAEP
                     VAECIVEDGGMCIDGFGEYVGHSLPDGMHELYAAPRMRQPDGWKAVAVAWKVTFTQVD
                     QESNTFTAIYFDKAEVECWVRLHKACDFRADITPLYAAPAVPVAVNDDMAYAFHHALS
                     DSSLGSDEIEEIKTGLRAAFANVTIQPEPIVPDEIGPNDSNTFDYVDGWNACRAAMLK
                     GDK"
     gene            1267..1632
                     /locus_tag="JNC24_004197"
     CDS             1267..1632
                     /locus_tag="JNC24_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HAY9160779.1"
                     /translation="MINRTKLEHILEYARQQKCIGQLCKIPPGDMVEIVEMAMRKAGN
                     SPVAPAGWISCSDAVPAEYCDVILLDDLGNVFPGSWDKVFCPTRGGNKMAFVDKDGVE
                     VESSTHWMPLPEPPQEVNQ"
     gene            2030..2242
                     /locus_tag="JNC24_004198"
     CDS             2030..2242
                     /locus_tag="JNC24_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HAY9160780.1"
                     /translation="MSHKCRLASYVPKGKEKQAMKQQKAMLIALIVICLTVIVTALVT
                     RKDLCEVRIRIGQTEVAVFVDYESRE"
BASE COUNT          627 a          496 c          628 g          578 t
ORIGIN      
        1 cccgaaaggg cggtaatgaa tggttacatt atttagaaaa aaatatccgc gaaagagtag
       61 aacaacagaa ttcctgtttc tcattctgtt tatcgtgttg atgacaccga tatccccgct
      121 aatttttgtc tggataatcg gaaaaataat tgagccagtt attgaattgt ataacgacgt
      181 ggtatgggcg tcattcaaca cactgcacaa taaaattaat ccgtataagg aaagctgata
      241 tggcactgac gaaaaaacaa cgtgcagaac tgcgcatgaa gttcggtggt cgctgcgctt
      301 attgcggctg cgaacttggc gaaaagtggc atgcagacca tgtaaaaccg gtcattcgtt
      361 ttgatggaaa tatgcttcac caggaacgtg acgatatatc caacatggta ccagcatgcc
      421 acccatgcaa tctgcacaag cattgcagta gcctggaaga ttatcgacga attatcagtg
      481 atggtcgtcg tgaattcctt gcgtccggga aaggcaaagc gctggttcgt atgggattgg
      541 ttgaaatgaa atctgacccg gttgtgttct ggtttgaaaa atatcaagaa ggggctacgg
      601 catgaccact attaccaaag agcgactgca atggctggct aacatttctg gccgcgatga
      661 tattgacgat atagacggcg gtgaaattcg tgaactggcg cttatcgctc tggcatcact
      721 ggaagcagag cctgtagcgg aatgcattgt tgaagatggg ggcatgtgta ttgacgggtt
      781 cggtgagtat gtgggtcact cgctgcctga tggaatgcat gagctttatg ctgccccgcg
      841 aatgcgacaa cctgatggat ggaaagccgt agctgtagcg tggaaggtga cgtttactca
      901 agttgaccag gaatctaata cgttcactgc tatatatttt gacaaagcgg aagttgagtg
      961 ttgggtacga ctgcataaag catgtgattt tcgggcagat ataacaccgc tttacgcagc
     1021 gccggcagtg ccggttgcag taaacgacga catggcttac gcattccatc acgcactgtc
     1081 agattcatcg cttggctctg atgaaatcga agaaattaaa accggtttgc gtgctgcctt
     1141 tgccaacgtc actatccaac cagagccgat agtgccggat gaaatcgggc caaacgatag
     1201 taatacgttt gattatgttg atggctggaa cgcctgccgc gctgccatgc ttaagggaga
     1261 taaataatga ttaatcgaac caaactggag cacattctcg aatatgccag gcagcagaaa
     1321 tgtattgggc aactttgtaa aattccacca ggagatatgg ttgaaatcgt ggaaatggcc
     1381 atgcgtaagg ctggcaactc tccggtagct ccggctggct ggataagctg tagtgatgca
     1441 gttcctgctg aatattgcga tgtgattctt ctcgatgatc tcgggaatgt attccccggt
     1501 tcctgggata aggttttttg ccccactcgt ggcgggaata agatggcttt tgtggacaaa
     1561 gacggcgttg aagtagagag ctcaactcac tggatgccgc taccggaacc accgcaggag
     1621 gtgaatcaat gacctggcct gaggcattca ccacggtagg aattgcgatg gcggtggcgc
     1681 tggtggtgta ttcgatttgc cgttggggtt aacaaacaaa aaccccggat tgatggtccg
     1741 gggtttttga aggaaacaaa acagaaacag caattgccgt tacctgttgt taccatggca
     1801 agtaaacgta tctcaggcga gcgcattgcg ccgttctgac gcagataaac tagcctggat
     1861 agatggtgct ggcaataaaa aatagcgttt tcttatcggt gtcggtaaga ttgctgcggg
     1921 tgcttgaggc tgtctgcctc gggtatgtca ctgtaaggca gacagagaaa agccccagtt
     1981 aacattacgc gtcctgcaag acgcttaaca ttaatctgag gcccaatcta tgtctcacaa
     2041 atgtaggtta gcctcttacg tgccgaaagg caaggagaag caggctatga agcagcaaaa
     2101 ggcgatgtta atcgccctga tcgtcatctg tttaaccgtc attgtgacgg cactggtaac
     2161 gaggaaagac ctctgcgagg tacgaatccg aatcggccag acggaggtcg ctgttttcgt
     2221 agactacgaa tctagagagt aagagtgacc aggcgggaga gtaatctccc gccacctttg
     2281 atgtgtcagg catcctcaac gcacccgcac ttaacccgtt tcggcggtt
//
LOCUS       DADMKF010000303         2296 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.308, whole genome shotgun sequence.
ACCESSION   DADMKF010000303 DADMKF010000000
VERSION     DADMKF010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2296
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.308"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(<1..431)
                     /locus_tag="JNC24_004199"
     CDS             complement(<1..431)
                     /locus_tag="JNC24_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAY9160781.1"
                     /translation="MVKSMKTNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     ISHDVFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIR"
     gene            488..757
                     /locus_tag="JNC24_004200"
     CDS             488..757
                     /locus_tag="JNC24_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000937495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HAY9160782.1"
                     /translation="MLPQHSDIEIAWYASIQQEPNGWKTVTTQFYIQEFSEYIAPLQD
                     AVDLEIATEEERSLLEAWNKYRVLLNRVDTSTAPDIEWPANPVRE"
     gene            complement(1169..1726)
                     /locus_tag="JNC24_004201"
     CDS             complement(1169..1726)
                     /locus_tag="JNC24_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HAY9160783.1"
                     /translation="MNTAITFSNESPKVFFKDGRLVTTSQAVADYFDKQHKHVLAKID
                     TLDCSPEFTSANFSADVQTVEIGNGAERESRCYLITKDGFMFLVMGFTGKKAARLKEA
                     YIEKFNAMEEELHKRPPAAQNSPAPNNGCALLIHFDKHGQVEFTEKVPADAMVCTLER
                     FKFYLEQHGWIVARKEQLVERLMRF"
     gene            complement(1723..1998)
                     /locus_tag="JNC24_004202"
     CDS             complement(1723..1998)
                     /locus_tag="JNC24_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HAY9160784.1"
                     /translation="MTVFVLRGYIPHTTAKSVVGIGVSDNITTHSRVCGFFCVKHCHI
                     RIMVGRAGAEKSAPGFVMTGYANPVRLTTSLIGVRGGDHPINWSHHI"
     gene            2183..2296
                     /locus_tag="JNC24_004203"
                     /pseudo
     CDS             2183..2296
                     /locus_tag="JNC24_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="receptor for colicin S4; incomplete; partial in the
                     middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
BASE COUNT          650 a          471 c          544 g          631 t
ORIGIN      
        1 ttgcgtatta cacagctttt tgtagatata tttccaggca ggcagtgaag aaggtaaaac
       61 atggaaatgg aatcaaattg accatgtaac gccgcgggat aaggttcaaa aacatcatgg
      121 ctaattttat gtttaatttt tgattcccca gcccttgtag atgccgcgtt caggctagct
      181 tcgttcaaat ccattaaaga tatcagacta ctctcaggta cgtgagtaag gtaaaaccca
      241 gttccaacac caatatccag atggttgtta cctaaatgtt ccagaaagtg tggaagaagg
      301 tgttcctttg taggacatcc ccatgcaagc cgatttgata ctcccaaaac ccaccagtca
      361 taaagcttta gggtaagtgg tgtgtaaatt ttagccccat catctgtgtt tgttttcatt
      421 gatttcacca tgttatagtt ttatttgtga attaaatcaa ttatggcgat gaattacaag
      481 gggttaaatg ctgccgcagc atagcgatat tgaaatagcc tggtatgctt cgatacagca
      541 ggagccgaat ggctggaaga ccgtcaccac acagttctac atccaggaat ttagtgagta
      601 tattgcgcca ctgcaggatg ctgtagatct ggaaatcgca acggaggaag aaagatcgtt
      661 gctggaggca tggaataaat atcgggtatt gttgaatcgt gttgatacat caactgcacc
      721 tgatattgag tggcctgcaa atcctgtcag ggagtaatca ttgggattat gccgcagaca
      781 cgtcgtatgc aggaacgtgc tgcggttagt ttgtgagctt tcgatagtgg ttgttatttt
      841 tgcccttatt tgttccggag gccatggttc aatggtccgt ctgccccctg tggtgatgtc
      901 agcaaaatca gccactgcgc gaaccacaat agcccgggaa gatgctgaag atcaccaggt
      961 aaagctgtca gcgcagaaac tggaagaact gctcgcatca atggttaagg atgaggttga
     1021 tcgcaatgat gggatttatt gacgtcagcg agagcagaag gaagaactga ataacctgaa
     1081 tgatttacgc tcaatcagag cgatggcgat tagcggcaat cacgctcgat aaaattaacc
     1141 tcggtgatca tccggggttt tgcattcatt aaaaccgcat caacctttcc accaattgtt
     1201 ctttacgggc aacgatccac ccatgttgct ccagataaaa tttaaaccgt tccagagtgc
     1261 ataccatcgc atcggcgggg actttttctg tgaactcgac ctgaccgtgt ttatcgaagt
     1321 ggatcagtaa tgcgcatcca ttatttgggg cgggggagtt ttgtgctgca ggaggacgtt
     1381 tgtggagttc ttcttccatt gcgttaaatt tttcaatgta agcttctttt agtctggctg
     1441 cttttttgcc agtaaatccc attacgagga acatgaagcc gtcttttgtg atcaggtagc
     1501 atcgtgattc acgttctgcg ccgttgccaa tttcgacagt ctgaacatcg gccgaaaagt
     1561 tggctgatgt aaattcggga gagcagtcga gtgtgtcaat cttagctaaa acgtgtttat
     1621 gttgtttatc gaagtagtcg gcaacagctt gagaggtggt aaccaggcga ccatctttga
     1681 aaaagacttt tggtgactcg ttagaaaaag ttatagctgt attcatatgt gatgactcca
     1741 attaattgga tgatcaccac ctctgacgcc aatcagggag gtggtgagac gtacagggtt
     1801 ggcgtaaccg gtcatcacaa aacccggcgc ggatttctcc gcccctgcac gccccaccat
     1861 aatgcgaatg tggcaatgct taacgcaaaa aaaaccgcaa acgcggctat gcgttgtgat
     1921 gttatccgag acgccaatcc cgacaaccga ttttgcggtt gtgtgaggaa tatagccccg
     1981 gagaacaaaa actgtcaacg ggcaaaaaaa tttccgttat caggaatgta taaaattaaa
     2041 gcgatcgagc aaaaaatcct ttctgcaaaa ctaaaaaaga tttcaataaa atcaggcagt
     2101 taaaaacacg gaaaacggta taaaaaagcg cctcaaaatc ctgttttttc tttcagatac
     2161 aatgtcttat tgcatttttt aaaacatgtc agtgtggtac atggatatcg ataccatcgc
     2221 caattataaa ctgggcggtg cacagcaaca tgatagcgta cgcctcgatc cgtgggtgtt
     2281 tatgttctca gcagga
//
LOCUS       DADMKF010000304         2286 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.309, whole genome shotgun sequence.
ACCESSION   DADMKF010000304 DADMKF010000000
VERSION     DADMKF010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2286
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.309"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            <1..325
                     /gene="casB"
                     /locus_tag="JNC24_004204"
     CDS             <1..325
                     /gene="casB"
                     /locus_tag="JNC24_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse2/CasB"
                     /protein_id="HAY9160785.1"
                     /translation="VSANVKAIDERQPFAAQLAAVMSKGRFTRLSAVKTPDELLRQLR
                     RAVRLLNGSVNLDSLADGIFRWCQESDDLLNHHRRQQRPTEFIRIRWALEYYQAGDAD
                     NEQNQ"
     gene            337..1392
                     /gene="cas7e"
                     /locus_tag="JNC24_004205"
     CDS             337..1392
                     /gene="cas7e"
                     /locus_tag="JNC24_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas7/Cse4/CasC"
                     /protein_id="HAY9160786.1"
                     /translation="MTTFIQLHVLTAYPAANLNRDDTGAPKTVVLGGATRLRVSSQSL
                     KRAWRTSALFEQALAGHIGIRSGRIAREAATILIEKGIEDKKAIEWAAKIADYLGKAK
                     NDKKPKDPLTNAETEQLVHISPAEFDAVKALAHQLAEEKRAPKEEDLALLRKDRIAVD
                     IAMFGRMLANKPEFNVEAACQVAHAFGVSETIVEDDFFTAVDDLRQASEDAGAGHLGE
                     TGFGSALFYTYICIDKDLLVENLGGDEALANQTIRAFTEAALKVSPTGKQNSFASRAY
                     ASWALAEKGTDQPRSLAAAFYEPINGTRQLDVAVQRITTLRENMNTVYEQKTECASFD
                     VMNKQGSMKDVLDFICA"
     gene            1403..2149
                     /gene="cas5e"
                     /locus_tag="JNC24_004206"
     CDS             1403..2149
                     /gene="cas5e"
                     /locus_tag="JNC24_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cas5/CasD"
                     /protein_id="HAY9160787.1"
                     /translation="MSQYLIFQLHGPMASWGVDAPGEVRHTHELPSRSALLGLLAAGV
                     GIRRDDTERLNAFNRHYSLVVCASRNPRWARDYHTVQMPKEVRKARYFSRREELSDPE
                     LLSAIISRRDYYTDAWWMVAVATTADAPYSLEQLQDGLRHPVFPLYLGRKSHPLALPL
                     APLLLEGNACDALCNAYQQYQDHFHKLKVSLPKLQDECWWEGKHDGLVASKILRRRDV
                     PLNRQQWLFGERTVNQGPWLSKEEPCTSQE"
     gene            2131..>2286
                     /gene="cas6e"
                     /locus_tag="JNC24_004207"
     CDS             2131..>2286
                     /gene="cas6e"
                     /locus_tag="JNC24_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311637.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HAY9160788.1"
                     /translation="MYLSRITLHTGQLSPAQLLHLVDRGEYVMHQWLWDLFPGGKERQ
                     FLYRREEL"
BASE COUNT          569 a          570 c          613 g          534 t
ORIGIN      
        1 agttagcgcc aatgttaaag ccattgatga acggcagcct tttgccgcgc aactggcggc
       61 ggtaatgtca aaggggcgtt ttactcgcct gtcagcagta aaaacaccgg atgaattact
      121 gcgccagtta cgccgcgccg tcagattatt aaacggttcc gtcaatcttg actccctggc
      181 agatggcatt ttccgctggt gccaggagag cgacgacctg ctcaatcatc accgccgcca
      241 gcagcggcca accgaattta tccgtatccg ctgggcgctg gaatattacc aggctggcga
      301 cgccgataac gaacaaaatc aataaaggac tatctcatga caacatttat tcagcttcat
      361 gtgttaaccg cttaccctgc tgccaacctt aaccgtgatg acaccggtgc gccaaaaact
      421 gtggtgctgg gtggagcaac gcgtctgcgc gtttcctcgc aaagtctgaa acgtgcgtgg
      481 cgcacttctg cactttttga acaggcactg gcgggccata ttggtattcg cagtgggcgt
      541 attgcgcgtg aggcggcaac tatcctgatt gagaaaggga ttgaagataa aaaagccatc
      601 gaatgggcgg caaaaattgc tgattatctt gggaaagcta aaaatgacaa aaaaccaaaa
      661 gatccgctca ctaacgccga aactgaacaa ttagttcata tcagcccggc agaatttgac
      721 gccgtaaaag cgcttgctca tcaactggca gaagaaaagc gcgcgccaaa agaggaagat
      781 ctcgcgttgt tacgtaaaga ccgtatagcg gtagatatcg ctatgtttgg tcgtatgctg
      841 gcgaataaac ctgagtttaa tgttgaagcc gcctgccagg tcgcgcatgc gtttggtgtc
      901 agtgaaacga ttgtcgaaga tgattttttc accgccgttg atgatttgcg ccaggcctct
      961 gaagatgccg gtgccgggca tctcggagaa accggattcg gttcagcact tttctacacc
     1021 tatatctgca ttgataaaga tctgctggta gaaaacctcg gcggtgacga agcgttagct
     1081 aatcagacca tacgcgcttt tacggaagcc gcgcttaaag tttccccaac cggtaaacaa
     1141 aatagctttg ccagccgtgc ctatgcttcc tgggcgctgg cagaaaaagg caccgatcaa
     1201 ccacgctctc tggcggcggc tttctatgaa cccattaatg gcacacgtca gttagatgtg
     1261 gcggtgcagc gtattacaac gcttcgcgaa aacatgaata cggtctacga acagaagacc
     1321 gaatgcgcaa gttttgacgt gatgaacaaa cagggaagca tgaaggacgt gctggacttt
     1381 atctgcgcgt aaggggcggc ttatgagcca atatctgatt tttcagcttc atgggccgat
     1441 ggcgtcctgg ggtgtcgatg ctccaggtga agttcgtcat acccatgaac ttccttcgcg
     1501 ctcagcattg ctggggctac tggctgccgg ggtagggatt cggcgtgatg ataccgaacg
     1561 cttaaacgcg tttaatcgcc actattcact ggtggtttgc gccagccgta acccgcgctg
     1621 ggcacgggat tatcacacgg tccagatgcc aaaagaggtg cgtaaagcac gttatttcag
     1681 ccgtcgtgaa gagttgagcg accctgagct tttgagcgcg attatctccc ggcgcgacta
     1741 ctacaccgat gcctggtgga tggtggcggt ggcaacaaca gccgatgcgc cttacagcct
     1801 cgaacagttg caggacggtt tacgccatcc agtttttccg ctttatctgg ggcgaaaaag
     1861 tcatccgctg gcattacctc ttgcgccgtt actgctcgaa ggcaacgcgt gtgatgcctt
     1921 atgtaatgca taccaacagt atcaggatca tttccataaa ctgaaagtct cactcccgaa
     1981 acttcaggat gaatgctggt gggaagggaa acacgacggc ctggtcgcga gcaaaatatt
     2041 acgccgccgg gatgttcctt taaatcgtca gcagtggctg tttggggaac gcaccgtcaa
     2101 tcaggggccg tggctcagca aggaggaacc atgtacctct caagaataac cttacatacc
     2161 ggccagcttt cgcctgcgca gttgctgcat ctggtggatc gcggtgaata tgtgatgcat
     2221 cagtggctgt gggatctttt ccctggcggc aaagaaaggc aatttcttta tcgtcgggaa
     2281 gaactc
//
LOCUS       DADMKF010000305         2283 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.310, whole genome shotgun sequence.
ACCESSION   DADMKF010000305 DADMKF010000000
VERSION     DADMKF010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2283
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.310"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(<1..304)
                     /locus_tag="JNC24_004208"
     CDS             complement(<1..304)
                     /locus_tag="JNC24_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HAY9160789.1"
                     /translation="MAHIQLVKQTSSGLLLPATPESCDFLHQIKIGEWIHADFKRVCN
                     YAFHKRFFKLLQLGFDYWTPVGGAITPRERKLVSGFVDYLCESVGREHTPALSEAAE"
     gene            complement(343..630)
                     /locus_tag="JNC24_004209"
     CDS             complement(343..630)
                     /locus_tag="JNC24_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9160790.1"
                     /translation="MKIEELREIFSENGLYAVRVENGAIVSHCRIRCLQSQQRKSGAV
                     LFYFCNGLLTDGFILREDEFVTSLRVLKEIGFKAGFLLLLKNKLIYNLEQD"
     gene            complement(880..1092)
                     /locus_tag="JNC24_004210"
     CDS             complement(880..1092)
                     /locus_tag="JNC24_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HAY9160791.1"
                     /translation="MLCTCRLASYMPKGKEKQAMKQQKAMLIALIVICLTVIVTALVT
                     RKDLCEVRIRTGQTEVAVFVDYESRK"
     gene            complement(1263..1928)
                     /locus_tag="JNC24_004211"
     CDS             complement(1263..1928)
                     /locus_tag="JNC24_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HAY9160792.1"
                     /translation="MTVADFKRPKLELPNGANKLLLHSCCAPCSGEVMEALQASGIDY
                     TIFFYNPNIHPQKEYLIRKDENIRFAEQHGVPFIDADYDTDNWFERAKGMEWEPERGI
                     RCTMCFDMRFERTALYAAENGFSVISSSLGISRWKNMQQVNDCGRRAVAHYPGMVYWD
                     YNWRKQGGSSRMIEISKREKFYQQEYCGCVYSLRDTNLHRKSQGRPLIKIGQLHYGKE
                     EKE"
     gene            complement(2097..>2283)
                     /locus_tag="JNC24_004212"
     CDS             complement(2097..>2283)
                     /locus_tag="JNC24_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9160793.1"
                     /translation="GAVVEPFDWMLSVAKRETRLADDVRLLRQEEQERRRNIEKLIQI
                     ERNAKARIRRATKSRTE"
BASE COUNT          568 a          595 c          508 g          612 t
ORIGIN      
        1 gctctgcggc ttcgctcaga gctggcgtat gttcccggcc tactgattca cacaggtaat
       61 caacgaaacc ggacaccagt tttcgttcgc gaggcgtgat cgccccaccg accggagtcc
      121 agtaatcgaa acccagttgc aggagtttga aaaaacgctt gtggaatgcg tagttacaca
      181 cacgcttaaa gtctgcgtgt atccactcac ctattttgat ttgatgcaga aaatcgcaac
      241 tctccggcgt cgccgggaga agtaatccgg aagaggtttg tttgaccagt tgtatatgtg
      301 ccatcggctt tctccggtgg cacggtgtta cacagcagga gttcaatcct gctcaagatt
      361 gtagatgagt ttattcttca gcaaaagcag aaaaccagcc ttaaaaccaa tctctttcaa
      421 aacccgtaat gatgtgacaa attcgtcctc acgcaaaata aaaccgtccg tcagaagtcc
      481 attacaaaaa taaaataaca cagcaccgct cttcctttgt tgagattgca aacatctaat
      541 gcggcaatgg ctgacaatcg ctccattctc aacgcgcaca gcatagaggc cattttcact
      601 aaaaatttca cgcaattctt cgattttcat cttcagaatc cttccagata aatagctctc
      661 ccctgttcgg ggtccatccc tcttctccct gcgcgctact taagtgagtc gattctatct
      721 gcgaaggtgc gcgaatcaaa ttcaccggaa ataaacaaca aaaaacccgc cgaaacgctg
      781 agagatcaca gcgatgggcg ggttaagtgc gggtgcgttg aggatgcctg acacatcaga
      841 ggtgggcggg gatgggatca gctccccgcc cggtcactct tacttcctgg attcgtagtc
      901 tacgaagaca gcgacctccg tctggccggt tcggattcgt acctcacaga ggtctttcct
      961 cgttaccagt gccgtcacta tgacggttaa acagatgacg atcagggcga ttaacatcgc
     1021 cttttgctgc ttcatagcct gcttctcctt gcctttcggc atgtaagagg ctaacctaca
     1081 tgtgcaaagc atgaaattgg cctcagatta atgttaagcg tcttgccgga cgcgtaatgt
     1141 taactggggc ttttctctat ctgccttttg gtgttcatgc ctgaagcaga tagcctcaag
     1201 cacccgcagt cattctactt acctcgccaa tatgaaatca atcagaaagg tgccccataa
     1261 aatcactcct tctcttcttt accgtagtgg agttgaccaa ttttgataag agggcgtccc
     1321 tgagatttgc ggtgtagatt ggtatcgcgc agagaataca cacagccaca atattcctgc
     1381 tgatagaatt tttcgcgctt gctgatttca atcatacggg acgagccgcc ctgcttgcgc
     1441 cagttataat cccagtacac catgcccgga taatgcgcga cggctcgccg cccacagtcg
     1501 ttaacctgct gcatattttt ccagcgtgaa atgcccagtg aactgctgat cacactgaaa
     1561 ccattttcag cggcgtacag cgctgtccgc tcaaaacgca tgtcaaaaca catggtgcaa
     1621 cggatccctc gttcgggctc ccattccatt cctttggctc gttcaaacca gttgtctgtg
     1681 tcgtaatcag catcaataaa cggcacgccg tgttgttcag caaagcgaat attctcatcc
     1741 ttacgaatta aatactcttt ctgaggatga atgttcgggt tgtagaaaaa gatggtgtag
     1801 tcgattcccg aggcctgaag cgcctccatc acctcaccgg aacatggagc acagcaagag
     1861 tgcagtagta gtttgtttgc cccgtttggg agctccaatt tgggccgttt gaaatcagca
     1921 actgtcataa atatgtttat tggggtcata aaaatatcaa agagtgtagc attagaacgg
     1981 ggctatcgga aacagatgtg tgactgctcc ccgccctttc gggtggtctc ctgatgattt
     2041 gagggtgcag aaatccctcc ggttaaggat taaattttta acagtgctaa atttaattat
     2101 tcagttctgg attttgtcgc cctgcgtatc cgcgctttcg cgttacgctc aatctgtatc
     2161 agcttttcta tatttctccg cctttcctgt tcctcctggc gcaatagcct tacatcatct
     2221 gccagtctgg tttctctttt tgccacagag agcatccagt caaacggctc cacaaccgca
     2281 ccg
//
LOCUS       DADMKF010000306         2210 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.311, whole genome shotgun sequence.
ACCESSION   DADMKF010000306 DADMKF010000000
VERSION     DADMKF010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2210
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.311"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            <1..717
                     /locus_tag="JNC24_004213"
     CDS             <1..717
                     /locus_tag="JNC24_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9160794.1"
                     /translation="ILSPITSFDYIHFITTHPSGIKDTLAWLVNASKLMTEFDDNGKI
                     IFNINALKYTKASYFEILGEKYIKITTSSPWLLEKLGKYIFSSRAPQVLELAIGWRGA
                     LSESIKGVKFCIWFSVAWRTIEFIMSSERDLVNFLGDFSMDVAKAVIAGGVATAIGSL
                     ASFACVSFGFPVILVGGAILLTGIVCTVVLNEIDAQCHLSEKLKYAIRDGLKRQQELD
                     KWKRENMTPFMYVLNTPPVI"
     gene            727..1206
                     /locus_tag="JNC24_004214"
     CDS             727..1206
                     /locus_tag="JNC24_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9160795.1"
                     /translation="MRLHVKLKEFLSMFFMAILFFPAFNASLFFTGVKPLYSIIKCST
                     EIFYDWRMLILCFGFMSFSFLNIHVILLTIIKLFLIKKTKVVNFATDITIQLTLIFLL
                     IAIVIAPLIAPFVTGYVNANYHPCGNNTGIFPGAIYIKNGMKCNNEYISRKEDSAVK"
     gene            complement(1343..2128)
                     /locus_tag="JNC24_004215"
     CDS             complement(1343..2128)
                     /locus_tag="JNC24_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9160796.1"
                     /translation="MNLRRVCCICVLFSLLAGCVSESSIDEKKKKAQVTQSNINKNTP
                     QQLTDKDLFGNETTLAVSEEDIQAALDGDEFRVPLNSPVILVQSGNRAPETIMQEEMR
                     KYYTVSTFSGIPDRQKPLTCNKNKDKNENEDVASAENMNWMQALRFVAAKGHQKAIIV
                     FQDTLQTGKYDSGLKSIVWSDYKNQKLTDTMSLRYLVRFTLVDVATGEWAAWSPVNYE
                     YKVLPPLPDKNEASTTDMTEQQIMQLKQKTYKAMVKDLVNRYQ"
BASE COUNT          655 a          424 c          392 g          739 t
ORIGIN      
        1 atactcagcc caataacatc atttgattac attcatttta tcactacaca tccttcaggt
       61 ataaaagata cactcgcctg gttagttaac gcgagtaaat taatgactga gtttgatgac
      121 aatgggaaaa ttatatttaa cattaatgcc ttaaagtata caaaagcaag ttactttgaa
      181 atacttggag agaaatatat aaaaatcaca acatccagcc cctggctttt agaaaagctc
      241 ggtaaatata ttttctcttc acgagcgcct caagttctgg aactcgctat tggctggcga
      301 ggagctttat ctgaatcaat aaaaggagtt aagttctgta tctggttttc agtagcctgg
      361 agaaccatag aatttattat gagttcagag cgagatctgg ttaattttct tggcgatttt
      421 tcaatggatg tggccaaagc agttatagcc ggtggtgttg caaccgctat tggaagcctg
      481 gcttcttttg cctgtgttag ctttggcttt ccagtaattc ttgtcggagg agcaatttta
      541 ctgacaggga tagtgtgtac agttgtttta aatgaaatcg atgctcaatg ccatttatca
      601 gaaaaattaa aatatgcaat tagagatgga ctaaaacggc aacaggaact tgataaatgg
      661 aaaagggaaa acatgactcc atttatgtat gttcttaaca ctccacccgt gatatgagga
      721 ttaaccatgc gtttacatgt taaattaaaa gagtttcttt cgatgttttt tatggccata
      781 ttatttttcc ctgcattcaa tgcttctttg ttttttaccg gagttaaacc tctatattca
      841 ataataaaat gctccacgga aatattttat gattggagaa tgttgattct gtgctttggt
      901 ttcatgtcgt tttccttttt aaatattcat gtaattttac ttacgataat aaaattattc
      961 ttaatcaaaa aaacaaaggt agtcaatttc gccacagaca taacaataca gctaactctt
     1021 atatttttac taatagctat cgtaatagca cctttaatag ctccttttgt cacaggatat
     1081 gtcaatgcta attatcaccc ctgcggtaat aatacgggaa ttttcccagg agcaatctat
     1141 attaaaaatg gcatgaaatg taataatgaa tatatttctc gcaaggaaga ttcagcagtt
     1201 aaataattaa acattctaaa acaaacagtt tttaactccg gattccatgt cctttcttgc
     1261 actgtctttg tcaagaaaaa tttcttatgc aaagaatttt gtattatttc cgccagaaat
     1321 tcctggcggc agatataatt tattattgat agcggttaac caaatctttt accatcgctt
     1381 tataggtctt ttgcttaagt tgcatgattt gctgctctgt catatcagtc gtactggctt
     1441 cattcttgtc gggcaatggt ggaagcactt tatattcata attcaccggc gaccaggcag
     1501 cccactcacc tgtagctaca tccaccagcg tgaatcgtac cagatagcgc agtgacatcg
     1561 tgtcagtcaa tttttgattt ttataatctg accaaactat tgatttcaat ccagagtcat
     1621 attttcctgt ctgcaacgta tcctggaaaa caataatcgc tttctgatgc ccttttgcag
     1681 ccacaaaacg cagtgcctgc atccagttca tattctcagc actggcaaca tcctcgttct
     1741 catttttatc tttgtttttg ttacaagtca gaggcttttg cctgtccggg ataccagaga
     1801 atgtggaaac agtataatat tttcgcatct cctcctgcat aatggtttcc ggtgcgcggt
     1861 tgccggattg aacaagtatt acaggagaat taaggggaac gcggaactca tctccatcaa
     1921 gcgcagcttg aatatcctct tcggatacgg ccagagtggt ttcattaccg aataaatctt
     1981 tgtctgtcag ttgctgggga gtgtttttat taatattact ttgtgtgact tgagcttttt
     2041 tcttcttttc atcaatagaa gattcagaga cacatcctgc aagcagcgag aataatacac
     2101 atatacaaca aacccttctt aaattcataa tcggacactc cctcgcctgt cataatcatg
     2161 gtaatgttta gctaacgatt tttaattata tactccactt gaatcagaac
//
LOCUS       DADMKF010000307         2205 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.312, whole genome shotgun sequence.
ACCESSION   DADMKF010000307 DADMKF010000000
VERSION     DADMKF010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2205
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.312"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(263..463)
                     /locus_tag="JNC24_004216"
     CDS             complement(263..463)
                     /locus_tag="JNC24_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001597778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9160797.1"
                     /translation="MLCIKFEYLTDKMIKHVSDLLIKEGGFGDACNPKDIFIHATSPN
                     ATLKTAVTAEWFERNKAELGYW"
     gene            complement(463..735)
                     /locus_tag="JNC24_004217"
     CDS             complement(463..735)
                     /locus_tag="JNC24_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000816065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAY9160798.1"
                     /translation="MKQWRDDIKRFFATYFMINYETGMLEWIDGGEVKTRDDAYGNPM
                     IRYGTRDYYLKYVIWFLHHEVQATKKIIFRDGNKRNCHPNNLLLEI"
     gene            complement(738..1016)
                     /locus_tag="JNC24_004218"
     CDS             complement(738..1016)
                     /locus_tag="JNC24_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9160799.1"
                     /translation="MNKHTLTRVTKTAFDELNRIKNITGLPHSAVMKLAINRDINEQD
                     LLNSRLEAARHHIRIKQDDLDSLKSLNAFGLDLARLLSIKIHKLSQEV"
     gene            complement(1017..1574)
                     /locus_tag="JNC24_004219"
     CDS             complement(1017..1574)
                     /locus_tag="JNC24_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9160800.1"
                     /translation="MQYSEFRAMLQASRERNKHNSYAYTNNPTSYEVPTFSKSERKNI
                     EAVIRSITPRDRFMPVRKTTKNTIKNYLANFDSYEQLPSKLDDVFIGFCRSEGHPKYN
                     KKLFYLLKNLDEINASTVTNHLQRQATRLSYELPSDKYCALLAVMCAKLIGIVEHHIA
                     VGNIEPMENEQPDFEFDPYLVTEDF"
BASE COUNT          598 a          447 c          424 g          736 t
ORIGIN      
        1 aattgataca ccggaatcag cgaaagcctg caccatcata gcagtgtcgc tatcaatgaa
       61 aatctgtaat tcatattcgt tcatttttaa aatctccttt ttaccagacc tcctcaatcg
      121 ctctgtaacg agctaaacag tctttttcag gggtagcctg tacagtggta ttacccaccc
      181 taaaataaga tagttttttg cgttagaatg cgattgagag cgtctgataa gctgtttttc
      241 taatttctct taaagtctcg gactaccaat aacctaattc cgctttattt cgttcaaacc
      301 attccgctgt aactgctgtc tttagtgttg cgtttgggct ggtggcatga atgaagatat
      361 cttttggatt gcaagcatca ccgaaaccac cttctttgat gagcaggtct gatacatgct
      421 taatcatttt atctgtcaaa tattcgaatt taatacataa cattaaatct ccaggagtaa
      481 attgtttggg tggcaatttc ttttattacc atccctgaag ataatctttt tagttgcctg
      541 aacttcatga tgcaaaaacc agatgacata cttcagataa taatcacgag tgccataacg
      601 aatcattgga ttaccgtaag catcatctct tgtcttaacc tctccaccat caatccattc
      661 aagcattcct gtctcataat taatcatgaa gtaagttgca aagaaccgtt taatatcatc
      721 tcgccattgc ttcataatca gacctcctga gacagtttgt gaattttgat acttagtaac
      781 cttgctaaat ccagtccaaa agcatttaag gatttcagac tatcaaggtc atcttgctta
      841 atcctgatat ggtgtcttgc agcctctaag cgactattaa gcaaatcctg ttcgttaata
      901 tcacggttaa ttgcaagctt catcactgct gagtgtggca gacctgtaat gtttttgatt
      961 cggttcagtt catcaaaggc tgtctttgta actctggtta atgtgtgttt attcatttaa
     1021 aaatcctctg taactaaata ggggtcaaac tcgaaatctg gttgctcgtt ttccattggc
     1081 tcaatattcc caacggcaat atgatgttca acaatgccaa ttaatttcgc gcacatcaca
     1141 gcaagcaaag cgcaatattt atcgcttggt aattcgtaac taagtctggt tgcctgtcgc
     1201 tgaagatgat ttgtcacggt ggatgcgtta atttcgtcaa gattctttag cagataaaat
     1261 agttttttgt tgtatttcgg gtggccttca cttctacaga acccaataaa aacatcatca
     1321 agcttgcttg gtaactgctc gtaactgtca aagttagcca gataattttt aatagtattc
     1381 tttgttgttt tgcgtactgg cataaatctg tctcgtggcg taatgctacg aatgaccgct
     1441 tcaatgttct tacgttcgga tttgctaaag gttgggactt cgtagctggt ggggttgttt
     1501 gtgtaagcgt aggagttgtg cttgttgcgt tctcttgatg cctgaagcat cgctctaaat
     1561 tctgaatact gcattcatgt tctgactctc ataagatgtt attattctgt taacctatat
     1621 acaatattag cattttcaaa gactaatgtc aagtattttt aacattttcg taacatctgt
     1681 ggtataatgg gtttatgggg agagtaaaat atgaggcgtg gctatgtatt tggtcgcata
     1741 ttttgcattg cacacagacc tcttcaatcg ccctgtaacg agttaaacga cctcttcagg
     1801 tcaactcata aggtcaggta ctttttgtgt cagaatgcga ttgagagcgt cttacaagga
     1861 aaactaatgg taaatctccc tcctgagctg gggtcacctg atgtatggac tggtggaggt
     1921 attgggctga ttgcaggcct gctacttcgc tacgtcctcg tcagagtgct tgttgtcttt
     1981 aaactgttcg gtcgtacatg gacgcttgac cttaaggata acgaccacac agacccccgt
     2041 taaactcctt caaaacccct gcacgagatg acagatagaa gtctatggta caggggtgtc
     2101 tttagcattt tgtgctatct attaaaaaaa tagcttgaca gaaccggaaa gccgtatcta
     2161 tcaagcattc acctctctga cagtaatttc taaaagttct tattt
//
LOCUS       DADMKF010000308         2169 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.313, whole genome shotgun sequence.
ACCESSION   DADMKF010000308 DADMKF010000000
VERSION     DADMKF010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2169
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.313"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            167..1666
                     /gene="pitB"
                     /locus_tag="JNC24_004220"
     CDS             167..1666
                     /gene="pitB"
                     /locus_tag="JNC24_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HAY9160801.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPTDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRYWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGTQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
     gene            complement(1715..>2169)
                     /locus_tag="JNC24_004221"
     CDS             complement(1715..>2169)
                     /locus_tag="JNC24_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HAY9160802.1"
                     /translation="VPALVITAFVARRLLRFRHMLACRRRGLIVLTDRYPQDQIPGAY
                     DGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDLVIKLNVDLEVACARKPDHKRES
                     LARKIAITPQLTFGGAQLVDIDANQPLEQVLVDAEKAITDFMTARGYH"
BASE COUNT          507 a          526 c          568 g          568 t
ORIGIN      
        1 acctgtgttt tgattatttc taaaggtttt gaatgaatgc ttattgtctg atacacaaga
       61 aataacactc tttttatcgt taaaaaatga tatttcactt tgcccatgcc gttaaagtca
      121 ctgataatgc gtccgttcgt aaattcaaaa tggcgtaatc taatatatgc taaatttatt
      181 tgttggcctt gatatataca cagggctttt gttattgctt gctctggcat ttgtgttgtt
      241 ctacgaagca atcaatggtt ttcatgacac ggcgaatgcg gtggcaaccg ttatttatac
      301 tcgtgccatg caaccacaac ttgctgtggt gatggcggca ttttttaact tttttggcgt
      361 gttattgggc ggacttagcg ttgcctatgc cattgtccat atgttgccaa ccgatttgtt
      421 gctgaatatg gggtcaaccc acggcctggc gatggtcttt tccatgctgc tggcggcgat
      481 tatctggaac ctgggaacgt ggttcttcgg tttaccggcc tccagttcgc acaccttgat
      541 tggtgcgatt atcggcatcg gtttaaccaa cgcgctgtta accggctcat cggtgatgga
      601 tgcgttaaac ctgcgtgaag tgaccaaaat tttctcctcg ctgattgttt cccctatcgt
      661 cggcctggtc attgcgggag gcctgatatt cctgctgcga cgctactgga gcgggacgaa
      721 aaagcgtgac cgtattcacc gcattccgga agatcgcaaa aagaaaaaag gcaaacgtaa
      781 accgccattc tggacgcgta ttgcgctgat tgtttccgct gcgggcgtgg cgttttcgca
      841 cggcgcgaac gacggacaaa aagggatcgg cctggtgatg ctggtactgg tggggattgc
      901 ccctgctggc ttcgtcgtca atatgaacgc gtccggctat gaaattaccc gtacccgcga
      961 tgccgtcacc aacttcgaac actacctgca acagcatcct gaactgccgc agaagttgat
     1021 tgcgatggaa cctccattgc ctgcagcatc gactgatggc acgcaagtaa cagagtttca
     1081 ctgtcatccg gcaaatacct ttgatgcgat tgcgcgcgtt aaaacgatgc tgccaggcaa
     1141 tatggaaagt tacgagccgt taagcgtgag tcagcgcagc cagctgcgcc gcattatgct
     1201 gtgcatctct gatacctccg cgaagctggc gaaactgcca ggcgtcagta aagaagacca
     1261 gaacctgctg aaaaaactgc gcagcgatat gttaagcacc attgagtacg ctccggtgtg
     1321 gatcatcatg gcggtagcac tggcgctcgg cattggcacc atgattggct ggcgtcgtgt
     1381 agcgatgacc atcggtgaga agattggtaa gcgcggcatg acgtatgcgc aaggcatggc
     1441 ggcacaaatg acggcggcag tgtctatcgg ccttgccagt tatattggga tgcctgtctc
     1501 cacaacacac gtcctctcgt ctgcggtagc agggacgatg gtggtggacg gcggtgggtt
     1561 acagcgtaaa acggtaacca gcatcctgat ggcgtgggtg tttactttac cggcggcaat
     1621 ttttctttct ggtgggctgt actggatagc attgcagttg atttaacctt cctgaaaatg
     1681 cccggtcctg gcgatcgggc atttccattt ttgactagtg ataaccacgc gcggtcataa
     1741 aatccgtaat cgctttttct gcatcaacca acacctgttc cagtggctga ttggcatcga
     1801 tatcaaccag ttgtgcacca ccaaaggtta actgtggcgt tatggcaatc ttcctcgcca
     1861 gcgattcccg tttatggtcg ggtttacgtg cacaggcaac ttcaaggtca acattgagtt
     1921 tgatgaccag atcaggctta tggctcgcca tccagtgaaa cgctttacgt tcctggcttg
     1981 ccagccatga gacaaaacga ccaccttcaa cgttaggtgg gaacaccgta ccatcgtaag
     2041 cgccaggaat ttggtcctga ggataacggt cggttagaac aattaacccg cgacgacgac
     2101 aggcaagcat atgacgaaag cgcagtaagc gacgggcgac aaacgctgta attaccagcg
     2161 ccggaactg
//
LOCUS       DADMKF010000309         2101 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.314, whole genome shotgun sequence.
ACCESSION   DADMKF010000309 DADMKF010000000
VERSION     DADMKF010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2101
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.314"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(60..1025)
                     /locus_tag="JNC24_004222"
     CDS             complement(60..1025)
                     /locus_tag="JNC24_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HAY9160803.1"
                     /translation="MSEDKFLSDYSPRDAVWDTQRTLTDSVGGIYQTAAEFERYALRM
                     ASCSGLLRFGWSTIMETGETRLRLRSAQFCRVRHCPVCQWRRTLMWQARFYQALPKIV
                     VDYPSSRWLFLTLTVRNCEIGELGTVLTAMNAAFKRMEKRKELSPVQGWIRATEVTRG
                     KDGSAHPHFHCLLMVQPSWFKGKNYVKHERWVELWRDCLRVNYEPNIDIRAVKTKTGE
                     VVANVAEQLQSAVAETLKYSVKPEDMANDPEWFLELTRQLHKRRFISTGGALKNVLQL
                     DRETNEDLVIADDVGDGTDDGKRTAFVWDSGKRRYKRAPEKDKSD"
     gene            complement(1891..>2101)
                     /locus_tag="JNC24_004223"
     CDS             complement(1891..>2101)
                     /locus_tag="JNC24_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9160804.1"
                     /translation="RSEAVIPDRFKNLILLIDGLSFGTFGFHPHEGFEDELILYIQKT
                     NERVKTLFVKIDLNGIKFGFLRTHS"
BASE COUNT          582 a          551 c          439 g          529 t
ORIGIN      
        1 aaaaaaaata aaccaatagt cttatttcca ttcaggtatt caatattaat attcatacgt
       61 taatccgatt tatccttctc aggggcgcgt ttgtaacgcc gtttacctga atcccagaca
      121 aacgccgtcc gcttcccgtc atcagtgcca tcccctacat cgtcggcaat gacaagatcc
      181 tcattggttt ctcgatccaa ctggaggacg ttttttagcg ccccaccggt cgagataaaa
      241 cggcgcttgt gaagctgccg cgtcagctca agaaaccact caggatcgtt tgccatatct
      301 tccggtttaa cggagtattt cagcgtttca gcaaccgcgc tttgcagttg ctcggcaacg
      361 ttggccacaa cctcacctgt cttagttttt actgcccgaa tatcgatatt cggctcatag
      421 ttcacccgca agcaatcgcg ccagagttct acccaacgtt cgtgcttaac gtagttcttc
      481 cctttaaacc aagaaggttg caccatcagc agacagtgaa aatgcggatg tgcgctgcca
      541 tccttacctc gcgtcacctc cgtagccctg atccacccct gaacaggtga tagctccttt
      601 cgcttttcca ttcgcttaaa cgccgcattc attgctgtaa ggactgttcc aagttcacct
      661 atctcgcagt tcctgacagt taacgtcaga aacaaccatc gggaagacgg gtaatccaca
      721 acgattttcg gtagagcctg ataaaaacgg gcttgccaca tgagggttct tctccactgg
      781 cagacagggc aatgacggac acggcaaaat tgcgcactac gaagccgtag gcgcgtttct
      841 ccggtttcca tgatggtaga ccaaccaaaa cgtaacaaac cgctacagga ggccatacgg
      901 agtgcatagc gctcgaattc agcagcagtc tggtagatac ccccgacaga atcggtaagc
      961 gtgcgctggg tatcccaaac tgcatcacgg gggctgtagt ccgaaaggaa tttatcttct
     1021 gacattaagc ctgctccgat ctgggcaggc tgtgatttga tgtggtatat tcatcacgta
     1081 cctgtgcatt gcgagccagt acatgtcacc agagtttgcc gctgaggtga cacattcgaa
     1141 atgttgggga gtgttgacgc actccccaac gccgattaaa aacgcctcaa ttctgctcca
     1201 ttttctttga cctgtcaaaa cccactcaaa gcctcgtcat tactgggcta aaccactatt
     1261 tcatatccgc catttatccc ttaagtataa atacaaggat cacgcgcgag aattgcgcaa
     1321 aaaataacca caattttaac cccccaaaac aagcaccccc gaacggctta ccccaacctt
     1381 ttccacacac ccgatcacca aaacagggaa taaaaacaaa accctgtctt acaggtggat
     1441 ggcctcactt cgtgaaacca tccaccccgc ataagttttt taatcggtgg tgtcgtcaat
     1501 cactaaatcg gaaccccaaa ggggaccccc acgatttagc atttgacgag attaactctc
     1561 ggagatggca tcatgctttt aacggacttt aaaaaacggt gaacggatgc ggattttaat
     1621 ccgtgtctgt gaggcgttga gcaacgagca aagcgaggcg gtagaaagag agccagcggc
     1681 aggctcagga cgataatccg gggaaaatat accactcgca gagtgttaac atttgacatc
     1741 ggattgtcta tcctgcattg gtgtcgagtt ggcaaggctg aggataatgc tgaggataat
     1801 gctgaggata atgcaggata gacagagcac tagagattgc atgcaatctc gtacctatca
     1861 cctcagcctg ccgctggctc ttttccagaa ctaagaatgc gttcttaaaa atccaaactt
     1921 gataccgttt aaatctattt tcacaaaaag agtttttacc ctctcgtttg ttttctgaat
     1981 atataaaatt aattcatcct caaaaccttc gtgcggatga aaaccaaatg tgccaaaact
     2041 taatccatca ataagcaaaa taaggttttt aaacctgtca ggaatcaccg cttcacttct
     2101 t
//
LOCUS       DADMKF010000310         2093 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.315, whole genome shotgun sequence.
ACCESSION   DADMKF010000310 DADMKF010000000
VERSION     DADMKF010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2093
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.315"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(<1..138)
                     /locus_tag="JNC24_004224"
     CDS             complement(<1..138)
                     /locus_tag="JNC24_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HAY9160805.1"
                     /translation="MVERTMAFKHYDVVRAAPPSDLAEKLTHKLKEGWQPFGSPVAIT
                     PY"
     gene            384..719
                     /gene="iraM"
                     /locus_tag="JNC24_004225"
     CDS             384..719
                     /gene="iraM"
                     /locus_tag="JNC24_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HAY9160806.1"
                     /translation="MKWIVIDTVIQPSCGISFSVIWSKIKLIIWYQSDAFLPPESIFT
                     LTHTGIMLNNKVLPVTIYNVVPFNKTFWNSIKNSQECPTNTDNVLNECFNNRCTLQIC
                     PYGLKQQSP"
     gene            complement(1000..1131)
                     /locus_tag="JNC24_004226"
     CDS             complement(1000..1131)
                     /locus_tag="JNC24_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001611307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 domain-containing protein"
                     /protein_id="HAY9160807.1"
                     /translation="MIAYCFCEWHCSSRIMSVLADGVLVAGVVVVAFPLLKKKTPDC"
     gene            complement(1631..1707)
                     /locus_tag="JNC24_004227"
     tRNA            complement(1631..1707)
                     /locus_tag="JNC24_004227"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1671..1673),aa:Arg,seq:tct)
     gene            complement(1808..1883)
                     /locus_tag="JNC24_004228"
     tRNA            complement(1808..1883)
                     /locus_tag="JNC24_004228"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1848..1850),aa:Ile,seq:cat)
     gene            complement(1986..>2093)
                     /locus_tag="JNC24_004229"
     CDS             complement(1986..>2093)
                     /locus_tag="JNC24_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HAY9160808.1"
                     /translation="YDALVTRFDIEEAWAERQLKKVTRLIFPESVVNSS"
BASE COUNT          677 a          522 c          370 g          524 t
ORIGIN      
        1 ataaggggtt atggccaccg gactaccaaa cggctgccag ccctctttca gtttatgtgt
       61 cagcttttcc gcaagatctg acggcggcgc cgccctgaca acatcatagt gtttaaatgc
      121 catggttctt tccaccatct gaaaaataat tctttaaaat acctgacatg taatacagaa
      181 aaaacacaaa accatacctt aaataaaaac ctgattatca agcagatatg catggataaa
      241 ctacaagacg agatataaac caccctgcat ttaaataaac aataaacaac atcagaaaaa
      301 taattctgct ctatggttta cattcaaaaa tatcatttat acttttcaga acatcaccag
      361 caaggcataa acaaggaaac taaatgaagt ggattgtgat tgatacagtt atccagccat
      421 catgcggaat atctttttca gtcatatgga gtaaaataaa attaataatc tggtatcaat
      481 cggatgcttt cttacctcct gaaagtatat ttacactgac tcacacaggc atcatgctca
      541 ataacaaagt gctacctgta accatttaca acgtagtacc attcaataaa acattctgga
      601 attcaatcaa aaatagccag gaatgcccta caaatacaga taacgtattg aatgaatgct
      661 ttaataaccg ttgcactctg caaatatgtc cttatggact aaaacaacaa agtccataag
      721 gagtttactc acatctgaca aaatcaatat aaacagcccc tccggagagg ggctggagag
      781 tggcgctatg tgccattgca tggtgccggg tgcctcccgg tgaattcagt accagcacct
      841 gaatccgcga ttatcccata tacctactcg ctgattgccc ctccgcacag ggggattcac
      901 catgccagtt tcttttaaca aactccccgc aaaccagaca acagtcaacc gcctgaattg
      961 tgaagtattt aaaaatttct cccgctaact gatacccggc taacagtctg gcgttttctt
     1021 tttcagcaac gggaaagcaa caaccaccac acccgccacc agcacaccgt cagccagcac
     1081 tgacattatc cggctgctgc aatgccattc acaaaaacag taagcaatca ctttttaccg
     1141 taacaggtga taatccagat atgtatctac cccagatgag taatccgaag ttcatccata
     1201 ccacaggtcc tggctattct gttgtactcc tgaacaagag caaataattc tgaattagca
     1261 accatgaact catcgcaaac cctctgtata gcatcactat tcagaataat aacgtctctt
     1321 cccgaaagac gatcaggagt acagaacaaa actgtcaaac ggctgaaggc ctttgctcgt
     1381 gctgcattga ctatatcaat acgctgccta aggatgaaac accccgacgc ctcatcaata
     1441 ttcactctac ccacaccata tgaatgataa atatttaatg ctgaaaaaac cattagaccg
     1501 tataacaaac actcaatcaa tacttaacag aacttttatt tttgacaaac ataaaatatt
     1561 ttcaacaata tcctgagcca ggtatatttc agtataaggc tctgccggaa ggaatctgga
     1621 agaatgaata tggcgcgctg tactggattc gaaccagtga ccgattgctt agaaggcaat
     1681 tgctctgtcc ggctgagcta acaacgctga ataccgataa tggaccgcca tcggggaccc
     1741 gcccccgcac caacaaccct gttatcgtgt cgtctgctct tcctgataag ctaatggcgg
     1801 tttgtgatgg tggcccttgc tggatttgaa ccagcgacct ggcgattatg agtcgctcgc
     1861 tctcaccact gagctaaagg gccgggagca gaataataac ggtccgtaat taattccgca
     1921 ataaaaaacc cgctcggcgg cgggttgtag aaactcttct aacgtcaggc ataaaacgcc
     1981 catagttatg acgaatttac cacagattcc ggaaaaatca acctagttac cttttttaac
     2041 tgccgctcag cccatgcttc ttcaatatca aaccgagtca ccagcgcatc ata
//
LOCUS       DADMKF010000311         2084 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.316, whole genome shotgun sequence.
ACCESSION   DADMKF010000311 DADMKF010000000
VERSION     DADMKF010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2084
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.316"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            <1..1600
                     /gene="tssI"
                     /locus_tag="JNC24_004230"
     CDS             <1..1600
                     /gene="tssI"
                     /locus_tag="JNC24_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HAY9160809.1"
                     /translation="VLCDTVRYLPESFEIPWNPNTRTEVSTFCISQFRYSAQIRPSSV
                     VTKDYTFKRPGWAGRFDQEGQYQDYQRTQYEVYDYPGRFKGAHGQNFARWQMDGWRNN
                     AEVARGTSRSPEIWPGRRIVLTGHPQANLNREWQVVASELHGEQPQAVPGRSGSGTTL
                     NNHFAVIPADRTWRPQPLLKPLVDGPQSAVVTGPAGEEIFCDEHGRVRVKFNWDRYNP
                     SNQDSSCWIRVAQAWAGTGFGNLAIPRVGQEVIVDFLNGDPDQPIIMGRTYHQENRTP
                     GSLPGTKTQMTIRSKTYKGSGFNELKFDDATGKEQVYIHVQKNMNTEVLNNRTTDVIN
                     NHAEKIGNNQAITVTNNQIQNIGVNQIQTVGVNLVETVGSNQIIKVGSNQVEKVGIIR
                     ALTVGVAYQTTVGGIMNTSVALLQSSQVGLHKSLMVGMGYSVNVGNNVTFSVGKTMKE
                     NTGQTAVYSAGEHLELCCGKARLVLTKDGSIFLNGTHIHLEGESDVNGDAPVINWNCG
                     ATQPVPDAPVPKDLPPGMPDMRQF"
     gene            1676..>2084
                     /locus_tag="JNC24_004231"
     CDS             1676..>2084
                     /locus_tag="JNC24_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000508696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9160810.1"
                     /translation="MGGGKPAARQGDMTRKGLDIVQGSAGVLIGAPTGVACSVCPGGI
                     TYANPVNPVLGAKVLPGETDLALPCPLPFILFRAYSSYRTRTPAPVGVFGPGWKAPFD
                     IRLQIRDEGLILNDSGGRSIHFEPLFPGEISYSR"
BASE COUNT          511 a          528 c          649 g          396 t
ORIGIN      
        1 ggtcctgtgc gacaccgtgc gttatctgcc ggagtccttt gagatcccct ggaacccgaa
       61 cacccgtacc gaggtaagta ccttctgcat cagccagttc cgctacagcg cacaaatccg
      121 tccttcttcc gtggtgacca aagactacac ctttaaacga cccggctggg cagggcgttt
      181 tgatcaggaa ggccagtacc aggattacca gcgcacacag tatgaagtgt atgactaccc
      241 cggacgtttc aagggtgctc acgggcagaa ctttgcccgc tggcagatgg atggctggcg
      301 caacaacgca gaagtggcgc gcggaacaag ccgttctccg gagatatggc cgggacggcg
      361 aattgtgctg acggggcatc cgcaggcgaa cctgaaccgg gaatggcagg tggtggcaag
      421 tgaactgcac ggcgagcagc cgcaggcggt gccgggacgc agtggttcag gaaccacgct
      481 gaataaccac tttgcggtaa taccggcaga ccggacatgg cggccacagc cgttgctgaa
      541 accgctggtg gatggcccgc agagcgccgt cgtgacggga ccggcaggcg aggaaatctt
      601 ctgtgatgaa catggccgcg tgcgggtgaa atttaactgg gaccgctata acccgtcaaa
      661 ccaggacagt tcatgctgga tccgtgtggc acaggcgtgg gcaggcaccg gattcggtaa
      721 ccttgcgata ccgcgcgtgg gtcaggaggt cattgtggac ttcctcaacg gcgatccgga
      781 ccagccgatt attatggggc gtacctacca ccaggaaaac cgcacacccg gtagcctgcc
      841 ggggacgaag acgcagatga ccattcgttc gaaaacctat aagggcagcg ggtttaatga
      901 actgaagttt gacgatgcga cagggaaaga acaggtctac atccacgtgc agaagaacat
      961 gaacaccgag gtgctgaata accgcaccac ggatgtgata aacaaccatg ccgaaaaaat
     1021 aggtaacaac caggcgatca ccgttaccaa taaccagatc cagaacattg gcgttaatca
     1081 gatacagacg gttggtgtca acctggtgga aacggtgggc agtaaccaga ttatcaaagt
     1141 gggatcaaac caggttgaaa aggtggggat cattcgtgcg ctgacggtgg gtgtggctta
     1201 ccagacgacg gtaggcggca ttatgaatac ctcggtggcg ttgttgcagt cctcacaggt
     1261 agggctgcat aaatcactga tggtgggaat gggctacagc gtcaatgtag ggaataacgt
     1321 caccttctcg gtgggcaaga cgatgaagga aaacaccgga caaacagcag tttattctgc
     1381 cggtgagcat cttgaactct gctgtggtaa ggcaaggctg gtgctgacga aggacggaag
     1441 catatttctc aacggtacgc acattcatct ggaaggggag tcggatgtga acggtgatgc
     1501 gccagtgatt aactggaact gtggtgccac acaacctgta ccggatgcgc ctgtgccgaa
     1561 agatttaccc cccggaatgc cggatatgcg gcaattttga gtcaggtgca aaaaattaaa
     1621 acggaactgg tgcagtgacc agatgaacca tattaaaagc agaaggaatt gattcatggg
     1681 cggcggaaaa ccggcggcgc ggcagggcga catgacccgc aagggactgg atattgtgca
     1741 gggttcagcg ggtgtgctga taggtgcgcc gacgggagtg gcctgttcgg tgtgtccggg
     1801 agggattacc tatgctaacc cggtgaaccc ggtgctgggt gcgaaggtgc ttccgggcga
     1861 gacggacctt gcgctgccct gcccgctgcc gtttattctt ttccgcgcct acagcagcta
     1921 ccggaccaga acacccgcgc cggtgggggt gtttggtccc ggctggaaag cgcccttcga
     1981 tatccgctta cagatacgcg atgaaggcct gatactcaac gacagcggcg gcaggagcat
     2041 tcactttgag ccgctgtttc ccggcgagat aagctacagc cgca
//
LOCUS       DADMKF010000312         2069 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.317, whole genome shotgun sequence.
ACCESSION   DADMKF010000312 DADMKF010000000
VERSION     DADMKF010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2069
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.317"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(36..974)
                     /gene="mdh"
                     /locus_tag="JNC24_004232"
     CDS             complement(36..974)
                     /gene="mdh"
                     /locus_tag="JNC24_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HAY9160811.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKTCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGEEFVNK"
     gene            1409..1879
                     /gene="argR"
                     /locus_tag="JNC24_004233"
     CDS             1409..1879
                     /gene="argR"
                     /locus_tag="JNC24_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HAY9160812.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
BASE COUNT          563 a          525 c          442 g          539 t
ORIGIN      
        1 gtctgtgctc cggtttttta ttattcgcta atcaattact tattaacgaa ctcttcgccc
       61 aaggcgatat ctttcttgag tgtatccagc ataccttcca gcgcgttctg ttcaaatgcg
      121 ctcagggtac cgatagattt acgctcttcc acgccgtttt tacccagcag cagcggttga
      181 gagaagaaac gggcgtactg accatcgcct tcaacgtagg cacattcgac aacgccttgt
      241 tcgccctgca gtgcacgaac cagagacaga ccaaaacgtg cagctgcctg gcccatagac
      301 agggttgcag acccgccacc ggctttcgct tcaaccactt cagtacccgc gttctggatg
      361 cgtttggtca gatcagccac ttcctgctcg gtaaaactaa cgccaggaac ctgtgacagc
      421 agcggcagaa tggtaacacc agagtgaccg ccaataaccg gcacttcaac ttcgcctggc
      481 tgtttgcctt tcagttccgc aacaaaagtg ttggaacgaa tgatgtccag cgtggtaacg
      541 ccgaacagtt tgtttttgtc ataaacaccg gcttttttca gcacttcagc agcaatcgca
      601 actgtggtgt taaccgggtt agtgataata ccaatgcacg ctttcgggca ggttttcgca
      661 acttgctgta ccaggttttt cacgatgccg gcgttaacgt taaacaggtc ggaacgatcc
      721 atacccggtt tacgcgctac acctgcagag ataagaacga catctgcgcc ttccagcgcc
      781 ggagtcgcat cttcaccaga aaaacctttg attttcacag cagtagggat atggctcaga
      841 tcgacagcca caccgggagt cactggagcg atatcataca gagagagttc tgaacctgaa
      901 ggcagttggg tttttaacag tagtgcaagc gcctggccaa taccgccagc agcgccgagg
      961 actgcgactt tcatcctaaa ctccttatta tattgataaa ctaagatatg ttgctccgct
     1021 gccgcgacct taatccacaa aattgccgtt tacaatgacc acatctcaag aatgtgtagt
     1081 cacgcaagtt tagcgtttat gcatttaatt gccgtaatca ggaacctaac ttacgtaatt
     1141 aacagtcgct tatgcattag cgcaacattc caacaggtgg tgacaatata ccctaccgtt
     1201 cagccaaaac aacatcaatt tgataacaat taatttactt ttaaacagaa tttgcatgcc
     1261 gtgacgcagg catgtttctc aataacgaaa tttgataaaa tcccgctctt tcataacatt
     1321 atttcagcct tcttcagggc tgactgtttg cataaaaatt catctgtatg cacaataatg
     1381 ttgtatcaac caccatatcg ggtgacttat gcgaagctcg gctaagcaag aagaactagt
     1441 taaagcattt aaagcattac ttaaagaaga gaaatttagc tcccagggcg aaatcgtcgc
     1501 cgcgttgcag gagcaaggct ttgacaatat taatcagtct aaagtctcgc ggatgttgac
     1561 caagtttggt gctgtacgta cacgcaatgc caaaatggaa atggtttact gcctgccagc
     1621 tgaactgggt gtaccaacca cctccagtcc attgaagaat ctggtgctgg atatcgacta
     1681 caacgatgca gttgtcgtga ttcataccag ccctggcgcg gcgcagttaa ttgctcgcct
     1741 gctggactca ctgggcaaag cagaaggtat tctgggcacc atcgctggcg atgacaccat
     1801 ctttaccacc cctgctaacg gtttcaccgt caaagacctg tacgaagcga ttttagagct
     1861 gttcgaccag gagctttaat ctctgccccg tcgcttctgg cggcggggaa aatgttgctt
     1921 attcctctca accccctgct ttcccctgcg attaatttaa cgaatagtgc gttttaccgc
     1981 gacatatcat tcacacaatg aatacatacg gtaaaaaaag cacattatgt aaaattcatt
     2041 atctaattga aaatactaga attaacaat
//
LOCUS       DADMKF010000313         2050 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.318, whole genome shotgun sequence.
ACCESSION   DADMKF010000313 DADMKF010000000
VERSION     DADMKF010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2050
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.318"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            1..536
                     /locus_tag="JNC24_004234"
                     /pseudo
     CDS             1..536
                     /locus_tag="JNC24_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706798.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
     gene            451..675
                     /locus_tag="JNC24_004235"
     CDS             451..675
                     /locus_tag="JNC24_004235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9160813.1"
                     /translation="MRKPIRTEQGANGAIRELEKFRQQGFTPEQVIRHSIANEYQGLF
                     APKGVRPETLLRQVNTVSLPDSAIPPGFRG"
     gene            682..1428
                     /locus_tag="JNC24_004236"
     CDS             682..1428
                     /locus_tag="JNC24_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAY9160814.1"
                     /translation="MKNIATGGVLERIRRLTPPHVTAPFRTVAEWREWQLAEGQKRSE
                     EINRLNRQLRVEKILNRSGIQPLHRKCSFANYQVQNDGQRYALSQAKSIADELMTGCT
                     NFAFSGKPGTGKNHLAAAIGNRLLKDGQTVIVVTVADVMSALHASYDDGQSGEKFLRE
                     LCEVDLLVLDEIGIQRETKNEQVVLHQIVDRRTASMRSVGMLTNLNYEVMKTLLGERV
                     MDRMVMNGGRWVNFNWESWRPNVSHSRVVK"
     gene            1443..1865
                     /locus_tag="JNC24_004237"
     CDS             1443..1865
                     /locus_tag="JNC24_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HAY9160815.1"
                     /translation="MAKPFTPEQREELKTRIVELVHQDGRVTIRQLSDETGISRASVG
                     RLCIELVASGDVYNSGYGLFPSEQARKDWQSARKKLSRVKVRKPVVVDPDLIWSLPDG
                     EIRRYDRRLNIICRECRKSEAMQRVLAFYQGNFQEAIL"
     gene            1866..>2050
                     /locus_tag="JNC24_004238"
     CDS             1866..>2050
                     /locus_tag="JNC24_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9160816.1"
                     /translation="MKLAIRLQLPLAFASKERSMEIKPEDELSNIVLFPVKEDDPRNQ
                     VNFLYEPSERPYCHHASV"
BASE COUNT          556 a          456 c          592 g          446 t
ORIGIN      
        1 ttattcgctt ctgggcatgg gcggatcaac aaatgataga cggtaatgca gattgtaacg
       61 ctcgcggcgt tacaaaaagt gcaatagatc gcatcacttt tatgtctggt tttgctgatg
      121 cgttaattca ggttggatgg ctggtcgaaa atgacggagg gctttctcta cctaactttg
      181 aacgtcataa cggaaaaagc tctaaaaaaa acgggcggtt acaaacgagc gagtaacaaa
      241 aatacgcgaa ctgaaacgaa aaggtaacgc tgccagcgtt acacaaacgg atcaaaaagc
      301 gttaccagag gaagaggaag aggaagatct aaatactgat ctccccctcg ccaaaaacga
      361 gcgtctaaaa aattcgagcc ggaggctatt gagctgcccg attggttgcc ggaaacactc
      421 tggcatgagt gggtccggtt cagacaggca ttgcgaaaac cgattcgaac ggagcagggc
      481 gctaacgggg cgatacggga actggaaaaa ttccgtcagc agggttttac acctgagcag
      541 gtgattcgac acagcatcgc caatgaatac cagggcctgt tcgcgccgaa aggtgttcgg
      601 cctgagacgt tgctccgaca ggttaacacc gtctcgttgc cggacagtgc gatcccgcca
      661 ggcttcaggg ggtaacggac catgaaaaat attgcgacag gcggcgttct ggaacgcatc
      721 cgccgactga ccccgccaca tgtaaccgcc ccattcagaa cggtagcgga gtggcgcgag
      781 tggcaacttg ctgaaggcca gaaacgtagc gaggagatca accgcctgaa tcgccagttg
      841 cgggtggaaa aaattctgaa tcgctcaggc atccagccgt tgcaccgtaa atgctcgttt
      901 gcgaattacc aggtgcagaa cgacggccag cgatacgcgt taagccaggc gaaatccatc
      961 gccgatgaac tgatgaccgg gtgtacaaat tttgcgttca gcggaaaacc tggtaccggg
     1021 aagaatcact tagcggcagc tatcgggaat cgcctgctga aagacggtca gacagtgatt
     1081 gtggttaccg tggctgatgt tatgagcgcc ctgcacgcca gctatgacga cgggcagtca
     1141 ggcgaaaaat ttttgcggga gctgtgcgaa gtggatctgc tggttcttga tgaaattggc
     1201 attcagcgcg agacgaaaaa cgagcaggtg gtgctgcacc agattgttga tcgccggaca
     1261 gcgtcgatgc gcagcgtggg gatgctgaca aacctgaact atgaggtgat gaaaacattg
     1321 ctcggcgagc gggtgatgga tcgcatggtc atgaacggcg ggcgctgggt gaattttaac
     1381 tgggagagct ggcgtccgaa tgttagccat tcgagggttg ttaagtagtt tcaggaggat
     1441 ttatggcgaa accttttact cccgaacagc gggaagaact gaagacgcga attgtggaac
     1501 tcgtgcatca ggacggtcgg gtcacgattc ggcagttgtc agatgaaaca ggtatcagtc
     1561 gtgcgtctgt cggtcgctta tgcatagaac tggtcgcaag tggtgatgta tataattctg
     1621 gctacggctt attcccgtct gaacaggctc gcaaggactg gcaaagcgcc cgcaaaaaac
     1681 tctcgagagt aaaggtgagg aaaccggttg ttgttgatcc ggaccttatc tggtcattac
     1741 ctgacggaga aatacgccgc tacgacaggc gcctgaatat aatctgtcgc gagtgccgga
     1801 agagcgaagc tatgcagcgt gtactggctt tctatcaggg taattttcag gaggcgatac
     1861 tgtgaatgaa attagctatc aggcttcaat taccgctggc attcgcatca aaggagagga
     1921 gcatggaaat aaaaccagaa gatgagttaa gcaatatcgt tttatttccg gtaaaagagg
     1981 atgaccctcg taatcaggtt aattttcttt atgagccatc ggaaagacca tattgccatc
     2041 acgcctctgt
//
LOCUS       DADMKF010000314         2005 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.319, whole genome shotgun sequence.
ACCESSION   DADMKF010000314 DADMKF010000000
VERSION     DADMKF010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2005
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.319"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            <1..389
                     /locus_tag="JNC24_004239"
     CDS             <1..389
                     /locus_tag="JNC24_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9160817.1"
                     /translation="FDLSLEIPLPPPGILSKTVVPGESSATVKQRVMAARERQFKRQN
                     KLNAWLDSPEIRQFCKLESEDAMWLEGTLIHLGLSIRAWQRLLKVARTIADIDQSDII
                     TRQHLQEAVSYRAIDRLLIHLQKLLT"
     gene            complement(414..752)
                     /gene="maoP"
                     /locus_tag="JNC24_004240"
     CDS             complement(414..752)
                     /gene="maoP"
                     /locus_tag="JNC24_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HAY9160818.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            871..1710
                     /gene="hdfR"
                     /locus_tag="JNC24_004241"
     CDS             871..1710
                     /gene="hdfR"
                     /locus_tag="JNC24_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HAY9160819.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     EVY"
     gene            complement(1806..1881)
                     /locus_tag="JNC24_004242"
     tRNA            complement(1806..1881)
                     /locus_tag="JNC24_004242"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1846..1848),aa:Trp,seq:cca)
     gene            complement(1890..1966)
                     /locus_tag="JNC24_004243"
     tRNA            complement(1890..1966)
                     /locus_tag="JNC24_004243"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1930..1932),aa:Asp,seq:gtc)
BASE COUNT          529 a          484 c          487 g          505 t
ORIGIN      
        1 gcttcgatct ctcactggag atcccattac caccccccgg cattttgagt aaaacggtag
       61 tgccgggaga aagcagcgcc accgttaaac aacgcgtaat ggccgccaga gagcgccaat
      121 ttaagcggca gaataaactg aatgcctggc tggatagtcc ggaaatacgc caattctgca
      181 aacttgagag cgaagatgcg atgtggttgg aaggaacact gatccatctg gggttatcga
      241 ttcgtgcctg gcagcggtta ttgaaagttg cacgaaccat tgctgatatt gatcagtctg
      301 acattatcac acgtcagcat ttgcaggagg cagttagcta tcgtgcgatt gaccgtttgc
      361 tcatccatct gcaaaaacta ctgacataaa aaaagggcat ttcgcccttt ttattaatca
      421 tcagaatcgg tgtagtcttc agcaccttca acctgcggtt taccgccgga aagggtgtga
      481 aaacgttttg gacgcttaat acgcgtcata tacttggacc acacgcgttc tgcttctgtc
      541 actggctcac gttcgccacg gcatactgct acgaagagtt tctcttcctc ggtaaccggc
      601 tcgcgtttgc caagatccaa ctcattgaag gcataaccat gacgctcaag cagttgtgcc
      661 tctttgatgg tgaaatcacc atgacgagag aatccacgtg gataatgttt attgtcgaaa
      721 tatcgattag tcgtcgtaaa gctttccgcc atcctgcacg ctcctaattc tttgaccgag
      781 ctagttatgg cgcggagtat tagttacgct tgacagagtg taaaacaaaa catttaaatc
      841 ataacgacaa ataattttgc ggagagcact gtggatacgg aattgttaaa aactttcctg
      901 gaagttagcc gaacgcgtca ctttggtcga gcggctgaat cgctctatct gacccagtca
      961 gcagtgagct ttcgaatcag gcaactggaa aatcaactgg gtgtgaacct tttcacccgc
     1021 cacagaaaca atatccgttt aaccgctgcc ggtgaaaaac tactgcctta tgcagaaacg
     1081 ctcatgagca cctggcaggc cgcccgcaag gaggtggcgc atacctcacg acataacgag
     1141 ttttctatcg gtgccagcgc ctcgttgtgg gaatgtatgc ttaatcagtg gttgggacgc
     1201 ttgtatcaaa atcaggatgc ccatacaggc ttacagttcg aagcgcgaat tgcccaacgg
     1261 cagtctctgg taaaacagct gcatgaacgc cagcttgatc ttcttattac cactgaagcg
     1321 cccaaaatgg acgaatttag tagtcagttg ctggggtatt tcactttagc gctttatacc
     1381 agtgcccctt ctaaactaaa gggagatctt aactatctgc gactggagtg ggggccagat
     1441 tttcaacagc atgaggcagg tttgatcggt gctgacgaag ttcccattct gacaaccagt
     1501 tctgctgaac tggcacagca acagattgcg atgcttaatg gttgcacctg gctacccgtt
     1561 agctgggcgc gtaaaaaagg cggcctgcat accgttgtcg atagcacaac actttcacgg
     1621 ccgctttatg ccatatggct gcaaaatagc gataaaaatg cgttgattcg cgatcttttg
     1681 aaaattaacg tgctggatga agtgtattaa tctgaatggc tggcaaggat gccggtagaa
     1741 ggatttactt cggagaggat tatttcagat aaaaaaaatc cttagctttc gctaaggatg
     1801 atttctggca ggggcggaga gactcgaact cccaacaccc ggttttggag accggtgctc
     1861 taccaattga actacgcccc taattagggt ggcggaacgg acgggactcg aacccgcgac
     1921 cccctgcgtg acaggcaggt attctaaccg actgaactac cgctccaccg aattctttta
     1981 caaccaccgg ttttatgacc ggctt
//
LOCUS       DADMKF010000315         1991 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.320, whole genome shotgun sequence.
ACCESSION   DADMKF010000315 DADMKF010000000
VERSION     DADMKF010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1991
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.320"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            1..936
                     /locus_tag="JNC24_004244"
                     /pseudo
     CDS             1..936
                     /locus_tag="JNC24_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1-like element ISEc1 family transposase"
     gene            947..1384
                     /locus_tag="JNC24_004245"
                     /pseudo
     CDS             947..1384
                     /locus_tag="JNC24_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307594.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
     gene            1384..1761
                     /locus_tag="JNC24_004246"
     CDS             1384..1761
                     /locus_tag="JNC24_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9160820.1"
                     /translation="MNQYIASYIEKMASFSTVLSETVSLANEYWHPETPPVVMLFSQV
                     GKTIVKNFYEMGDIEREKIFKHVEDGMVSLDDELATVVATGLIEAIVTATDSNSLLWQ
                     DLSKFLGDKSKSHALVWKNFGQE"
BASE COUNT          661 a          319 c          493 g          518 t
ORIGIN      
        1 attcctgttc acgataccat tgccagagtt gtatcctgta tcagtcctgc aaaatttcac
       61 gagtgcttta ttaactgaat gcgtgactgc cattcttcag atgataaaga cgtcattgca
      121 attgatggaa aaacgctccg gcactcttat gacaagagtc gccgcagggg agcgattcat
      181 gtcattagtg cgttctcaac aatgcacagt ctggtcatcg gacagatcaa gacggatgag
      241 aaatctaatg agattacagc tatcccagaa cttcttaaca tgctagatat taaaggaaaa
      301 atcatcacaa ctgatgcgat gggttgccag aaagatattg cagagaagat acaaaaacag
      361 ggaggtgatt atttattcgc ggtaaaagga aaccaggggc ggctaaataa agcctttgag
      421 gaaaaatttc cgctgaaaga attaaataat ccagagcatg acagttacgc aatgagtgaa
      481 aagagtcacg gcagagaaga aatccgtctt catattgttt gcgatgtccc tgatgaactt
      541 attgatttca cgtttgaatg gaaaggactg aagaaattat gcgtggcagt ctcctttcgg
      601 tcaataatag cagaacaaaa gaaagagcca gaaatgacgg tcagatatta tatcagttct
      661 gctgatttaa ccgcagaaaa gttcgccaca gcaatccgaa accactggca cgtggagaat
      721 aagctgcact ggcgtctgga cgtggtaatg aatgaagacg actgcaaaat aagaagagga
      781 aacgccgcag aattattttc agggatacgg cacatcgcta ttaatatttt aacgaatgat
      841 aaggtattca aggcagggtt aagacgtaag atgcgaaaag cagccatgga tagaaactat
      901 ctcgcgtcag tccttgcggg gagcgggctt tcgtaatctt tccctaggga gtcagccgaa
      961 tgttgggaag gatttgacgg atgaagagaa gaactgcttg gccggcggca gtacgggaag
     1021 cccgggagga tggggaccag aagatgagaa tagcgcaagg gatcgtatgc agcatgaacg
     1081 gtataaagat gaattgcgac agaaaatggg aaaacccgac gttacagatt caaatttgaa
     1141 aaatattata gacgatcttt atcggccaaa tgcaaaagta ggaagtggaa gcacagcaga
     1201 tgctgttcgt tatgaattgg ccacaggtga aaaagttggt ggtagaggac atgtattgaa
     1261 agcacaggat tatacaaggg ctttacagga ttggttggat aagaatccaa cagcatctcc
     1321 aggggatcga gcagcagcag aaaacgttct tcaggattta cagaatgctt tgggaggcaa
     1381 gtaatgaacc aatatattgc tagttatata gaaaaaatgg catcattttc cactgttttg
     1441 tctgagacag tctcattagc taatgaatac tggcatccag aaacgccacc ggtggttatg
     1501 ttgttctctc aggttggaaa aacgattgta aaaaattttt atgaaatggg ggatattgaa
     1561 agagaaaaaa tatttaaaca tgtcgaagat ggtatggtct cgcttgatga tgaattggcc
     1621 acagttgtag ctacagggct tatagaggca atagtcactg cgaccgatag caattcttta
     1681 ctttggcaag atttaagtaa atttctgggt gacaaatcaa agtctcatgc attagtgtgg
     1741 aaaaattttg gtcaggagtg atattattca agaagtatta agctcgctat tgagatctta
     1801 atgcttcttg agattaaatt aaagttagga cgaagtaatt gcaggtggaa ataaatccat
     1861 agtgacgtgt ggcaatggct attagatgtt atggtgtgat gggctttggt gatttaagag
     1921 ctaagttaga ttgccttatg aaaaagtatg gttatatatt taatgatgtg tatcatatat
     1981 ttaaataaaa a
//
LOCUS       DADMKF010000316         1939 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.321, whole genome shotgun sequence.
ACCESSION   DADMKF010000316 DADMKF010000000
VERSION     DADMKF010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1939
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.321"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(129..683)
                     /locus_tag="JNC24_004247"
     CDS             complement(129..683)
                     /locus_tag="JNC24_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HAY9160821.1"
                     /translation="MIYPTNTGKSGEHLRLTTLESVWIQGKLRMWGRWSYIGGGKTGN
                     MFNLMLTSKKLTKTAINEALRRMKKAGLNKSELEAFLRDMINGKQKSWLAHCTDAEAL
                     CIDRVISEVLAEHPGLISVLRQRYEGRGMTKRKMAELLNDAHPKWSLRTCERRIEHWL
                     KVAEFILYKPMVMAFGIEKKVIAF"
     gene            complement(680..970)
                     /locus_tag="JNC24_004248"
     CDS             complement(680..970)
                     /locus_tag="JNC24_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HAY9160822.1"
                     /translation="MVDLRKAARGQMCTVRIPGYCNHDPETSVLAHYRLAGTCGTAIK
                     PHDMQAAIACSSCHDLIDGRVKTSDYTKEELRLMHAEGVFRTQEIWRKEGYL"
     gene            complement(970..1569)
                     /locus_tag="JNC24_004249"
     CDS             complement(970..1569)
                     /locus_tag="JNC24_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HAY9160823.1"
                     /translation="MAHIQLIKQTSSGLLLPATPESCDFLHQIKTGEWIHADFKRVRN
                     YAFHKRFFKLLQLGFDYWTPVGGAITPRERELLSGFVDYLCESVGREHTPALSEAAEQ
                     YLNTVATRRTRDTALLKSFEAFREWVTIQAGFYTEHFYPDGSRGRRAKSIAFANMDET
                     EFQQVYKSVLNVLWNWILFRKFSSPEQVENVAAQLLEFA"
     gene            complement(1641..1910)
                     /locus_tag="JNC24_004250"
     CDS             complement(1641..1910)
                     /locus_tag="JNC24_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9160824.1"
                     /translation="MTFCLYVDITVCTYKPGGIVEKIKKLFSSKYAVIRRDDLSVIVE
                     MDYFPETKKSMMYRNGRKAIFLPMRVSDIMGNDKLLDELRVRASC"
BASE COUNT          484 a          511 c          394 g          550 t
ORIGIN      
        1 taacgtcctg tgccgttttt caggtataaa aaaacccgca taaagcgggc tctttcaaat
       61 gtccatgtct gctattcgcc tcgcggtaca gctttgcgaa gcgtacaaga attgaagcag
      121 tttttacgtc aaaaagcaat aacttttttc tctataccaa aagccataac cattggtttg
      181 tacaaaataa attctgccac ctttagccaa tgctcaatgc gtctttcaca ggttcttaaa
      241 ctccatttcg ggtgtgcatc attcagcagt tcagccattt tgcgtttggt catcccccgc
      301 ccctcatacc gttgccgaag gacgctaatc aatcctggat gctctgccag cacctcactt
      361 atgacccgat caatacataa cgcctctgca tcagtacaat gcgccagcca gctcttttgc
      421 ttaccgttaa tcatatcccg caaaaaagcc tcaagttcag acttgttcag acctgctttt
      481 ttcatcctcc ggagcgcctc gttaattgcc gtttttgtca gctttttaga ggtcaacatc
      541 aggttaaaca tattccccgt cttaccgcca ccaatatacg accagcgccc ccacatacgt
      601 agttttccct gaatccagac actttccagc gtggtgagac gaaggtgttc tccgcttttt
      661 cctgtattcg ttgggtaaat cataaatatc cctcctttct ccagatttct tgtgtgcgaa
      721 aaacaccttc tgcatgcatc aggcgtaatt cttctttggt gtaatcgctg gtttttaccc
      781 gcccgtcgat taaatcgtgg cacgagctac aggcaatcgc tgcctgcata tcgtgtggct
      841 ttatcgctgt tccgcacgtt cccgccagtc ggtaatgcgc cagcacagac gtttccggat
      901 cgtgattgca gtagccagga attctgacgg tgcacatctg cccccgcgcc gctttacgta
      961 aatccaccat tacgcaaact ccagcagctg cgcggccaca ttttcgactt gttccggaga
     1021 ggaaaattta cggaacagaa tccagttcca cagcacattc agaacagatt tataaacctg
     1081 ctgaaactcg gtttcgtcca tattcgcaaa agcgatggat tttgcccgac gcccgcggct
     1141 accgtccgga taaaaatgtt cggtgtaaaa tccagcctga atggttaccc actcgcgaaa
     1201 agcctcaaac gactttagca atgccgtatc ccgggttctg cgtgtcgcaa ctgtattcag
     1261 atattgctct gcggcttcgc tcagagctgg cgtatgttcc cgacctactg attcgcacag
     1321 gtaatcaacg aaaccagaca gcagttctcg ttcgcgaggc gtgatcgccc caccgaccgg
     1381 agtccagtaa tcgaatccca gttgcaggag tttgaaaaaa cgcttgtgga atgcgtagtt
     1441 acgcacacgc ttaaagtctg cgtgtatcca ctcacctgtt ttgatttgat gcagaaaatc
     1501 gcaactctcc ggcgtcgccg ggagaagtaa tccggaagag gtttgtttga tcagttgtat
     1561 atgcgccatc gtagttctcc gctggcgcag tagaatgggt gttcagcccg ttatgtagta
     1621 taccagaatt aatgccaata ctaacaggat gctctgactc gcaattcatc cagcagttta
     1681 tcatttccca taatgtcact taccctcatc ggtaaaaaaa ttgcctttcg accattacga
     1741 tacatcattg attttttggt ttcagggaag taatccattt cgactataac tgacaggtca
     1801 tcacgacgta tgactgcgta tttactacta aatagtttct ttattttttc cacgatgcct
     1861 ccaggtttat aagtacaaac ggttatatca acatagagac aaaaggtgat gctgccaact
     1921 tactgattta gtgtatgat
//
LOCUS       DADMKF010000317         1916 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.322, whole genome shotgun sequence.
ACCESSION   DADMKF010000317 DADMKF010000000
VERSION     DADMKF010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1916
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.322"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            2..286
                     /locus_tag="JNC24_004251"
     CDS             2..286
                     /locus_tag="JNC24_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001675603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY9160825.1"
                     /translation="MSISKLALENGINANLLFKWRQQWREGKLLLPSSESPQLLPVTL
                     DAAAEQPESLAEDPKTLSISCEVTFRHGTLRFNGNVSEKLLTLLIQELKR"
     gene            283..630
                     /gene="tnpB"
                     /locus_tag="JNC24_004252"
     CDS             283..630
                     /gene="tnpB"
                     /locus_tag="JNC24_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019104956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HAY9160826.1"
                     /translation="MIPLPSGIKIWLVAGITDMRNGFNGLAAKVQTALKDDPMSGHVF
                     IFRGRSGSQVKLLWSTSDGLCLLTKRLERGRFAWPSARDGKVFLTQAQLAMLLEGIDW
                     RQPKRLLTSLTML"
     gene            680..1916
                     /locus_tag="JNC24_004253"
                     /pseudo
     CDS             680..1916
                     /locus_tag="JNC24_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
BASE COUNT          436 a          542 c          564 g          374 t
ORIGIN      
        1 gatatccatc tcaaaacttg ctcttgaaaa tggcattaac gccaatctgt tgttcaaatg
       61 gcgacaacaa tggcgcgagg gaaagctgct attaccttct tcagagagcc cccagctact
      121 tcctgtgact ctcgatgcag ctgccgaaca gccagaatcg ctcgcagagg acccgaaaac
      181 cctcagtatc agctgtgagg taacgttccg gcacgggacg ctccgcttca atggcaatgt
      241 cagcgaaaag ctcctgactc tgctgataca ggaactgaag cgatgatccc gttaccttcc
      301 gggatcaaaa tttggctggt tgccggtatc accgatatga gaaatggctt caacggcctg
      361 gctgcgaaag tacagacggc gctgaaagac gatcccatgt ccggccatgt tttcattttc
      421 cggggccgca gcggcagtca ggttaaactg ctgtggtcca ccagtgacgg actgtgcctc
      481 ctgaccaaac ggctggagcg tgggcgcttc gcctggccgt cagcccgtga tggcaaagtg
      541 ttccttacac aggcgcagct ggcgatgctg ctggaaggta tcgactggcg acagcctaag
      601 cggctgctga cctccctgac catgctgtaa atctctttat cctggttgtc acagaataag
      661 cccggtaaaa tacgggctta tgaacgacat ctcttctgac gacatcttcc tgctgaaaca
      721 gcgcctggcc gaacaggaag cgctgatcca cgccctgcag gaaaagctta gcaaccggga
      781 gcgcgaaata gaccatctgc aggcgcagct ggataaactc cgccggatga acttcggcag
      841 tcgttccgaa aaagtctccc gccgtatcgc acaaatggaa gccgatctga accggcttca
      901 gaaagagagc gatacgctga ctggtagggt gtatgacccg gcagtacagc gtccgttgcg
      961 tcagacccgc acccgtaagc cgttccctga atcactaccc cgtgacgaaa agcgactgtt
     1021 gcctgcggcg ccgtgctgcc cgaactgcgg cggttcactg agctatctgg gcgaggatac
     1081 cgccgaacag ctggagttga tgcgtagcgc cttccgggtt atccggacgg tacgggaaaa
     1141 acatgcctgt actcagtgcg atgccatcgt gcaggcacct gcaccttcgc ggcccatcga
     1201 gcggggtatc gccggaccgg ggctgctggc ccgcgtgctg acctcgaagt atgcagagca
     1261 caccccgctg tatcgccagt cagaaatata cggccggcaa ggtgtggagc tgagccgttc
     1321 actgctgtcg ggctgggtgg atgcatgctg ccggctgctg tctccgatgg aagaggcgct
     1381 tcatggctat gtgctgactg acggtaagct ccatgttgat gacacgcctg tcccggtgct
     1441 gttgccaggc aataagaaaa cgaagaccgg gcggttgtgg cgtatgttcg tgacgaccgt
     1501 aacgccgggt caacgctggc gccggcggtg tggttcgctt acagcccgga cagaaaaggc
     1561 atccatccgc agacccatct tgcggggttc agtggtgtac tgcaggcgga tgcatacgcc
     1621 gggttcaacg agctgtaccg ggatggccgg ataacggaag ccgcctgttg ggctcacgcc
     1681 cgccgtaaaa tccacgatgt gcacgttcgc accccgtcag ccctgacgga ggaagcgctg
     1741 aaacggatcg gcgaactgta cgccatcgag gcagagataa ggggaatgac ggcggaacag
     1801 cgccttgccg aacgtcagtt gaaaacgaaa ccgctgctga aatccctgga aagctggctg
     1861 cgtgaaaaga tgaaaaccct gtcgcgacac tcagaactgg cgaaagcgtt cgcata
//
LOCUS       DADMKF010000318         1881 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.323, whole genome shotgun sequence.
ACCESSION   DADMKF010000318 DADMKF010000000
VERSION     DADMKF010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1881
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.323"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(161..496)
                     /locus_tag="JNC24_004254"
     CDS             complement(161..496)
                     /locus_tag="JNC24_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin transporter"
                     /protein_id="HAY9160827.1"
                     /translation="MNRKYYFNNMWWGWVTGGYMLYMSWDYDFKYRLLFWCISLCGMV
                     LYPVAKWYIEDTALKFTRPDFWNSVFFADTPGKMGLLAVYTGTVFILSLPLSMIYILS
                     VIIKRLSVR"
     gene            625..972
                     /locus_tag="JNC24_004255"
     CDS             625..972
                     /locus_tag="JNC24_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9160828.1"
                     /translation="MTFEQKKARAIALMDSKKMWRSNYAPPLLRILWRLGIRLPPLPF
                     MPFWQVTVLTGGLWGISWGCAMWFIYWGPSGMVAGEAIIISITGGFLSGLLMASFHWW
                     RRKVNRLPPWDDV"
     gene            complement(992..1582)
                     /locus_tag="JNC24_004256"
     CDS             complement(992..1582)
                     /locus_tag="JNC24_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9160829.1"
                     /translation="MTQSRRPSPLQRRVLIVLAALDEKRPGPVLTRDIERVLEQSGEA
                     PVYGPNLRASCRRLEDAGWLRTLHAPNLQLAVELTDAGRAVAQPLLLAEQDRIRAEQR
                     AAEVVVLPLVPAAGLPAGGTSATDLAVELNGITYQACRGDFVVRLDGSTCLQLWNKEG
                     WVVRREGDPLEVAQWLPACHDAGMEVRVQINESAAP"
     gene            complement(1579..1839)
                     /locus_tag="JNC24_004257"
     CDS             complement(1579..1839)
                     /locus_tag="JNC24_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001488293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9160830.1"
                     /translation="MDQEMTFSLSYEQLTRFAEKRIRECNLDSHGVTYLCESAKAGAV
                     LIFWHELAINGYTSMNAIKRQEIIDADHQRLRKLIWPEDDWK"
BASE COUNT          454 a          505 c          452 g          470 t
ORIGIN      
        1 catataatct tacagcgcaa gcttccccgt catacagacc tcgtgatcga gttaataaga
       61 ctatgataga actacaaact aacggagtag aacggcaact tttagataat tcttgtggca
      121 taagttctgg ggtatttaaa taaaatataa gacaggctgt ctatcttaca gacagccttt
      181 ttataataac agaaagaata tatatcatac ttaacggaag agataatatg aaaacagtac
      241 ccgtataaac agcaagcaat cccatttttc caggtgtatc agcaaaaaaa acgctgttcc
      301 agaaatcagg ccgggtaaat ttcagagctg tatcttcaat ataccatttt gcaaccggat
      361 acaaaaccat tccgcagaga gaaatacacc agaacagtaa tctgtattta aaatcataat
      421 cccatgacat atacagcata tatcctcccg tcacccatcc ccaccacata ttattaaaat
      481 aatattttct gttcatctgc cagttttcct ttcttttgca cttttgtcag tgtactgatg
      541 catgacaaca taaccacagc atgataataa taatcaataa caataagctg tgtcacgttt
      601 acatcatgga acggaggtgc tcctgtgact tttgagcaga aaaaagcccg ggccattgcc
      661 ctgatggaca gcaaaaagat gtggcggagc aattatgccc ctcctcttct gcgtattctc
      721 tggcggctgg gtatccggct tccgccatta ccctttatgc cgttctggca ggtcacggtg
      781 ctgacggggg gcttatgggg catttcctgg ggatgtgcaa tgtggtttat ctactggggg
      841 ccgtcaggaa tggtggcagg tgaggccatt attataagta tcacgggggg ttttttgtcc
      901 ggactgctca tggcctcttt ccactggtgg cgcaggaaag ttaaccgact gccaccatgg
      961 gatgatgtgt aaaccgtgca caggcgtgtt cttacggagc ggcactttca tttatctgta
     1021 cgcgaacctc cattcctgca tcgtgacagg ccggcagcca ctgcgccact tccagcggat
     1081 cgccctcccg gcgtaccacc cagccttctt tattccataa ctgcagacag gtgctgccgt
     1141 cgagacgcac cacaaaatcc ccacggcagg cctgatacgt tatgccgttc agctccaccg
     1201 ccagatcagt ggcagaagtg ccgcctgcgg gcagacctgc tgccgggacc agcggcagta
     1261 cgacgacttc tgccgcacgc tgttctgccc ggatgcggtc ctgttctgcc agcagcagag
     1321 gctgtgccac ggcccggccg gcgtccgtca gctccactgc cagctgcagg ttcggagcgt
     1381 gcagggtacg cagccagccg gcatcctcca gacgacggca ggaggcgcgc aggtttggcc
     1441 cgtagaccgg ggcttccccg ctttgctcca gcacccgttc tatgtcccgc gtcagcacgg
     1501 gtcctggccg tttttcatca agggcagcca gcacaatcag cactctccgc tgcagtggcg
     1561 acggtcggcg gctctgagtc atttccagtc atcctccggc cagatgagct ttctgaggcg
     1621 ctgatggtcg gcgtcaatga tctcctgtct ctttatggca ttcattgagg tatagccatt
     1681 tatggccagt tcatgccaga agatcagcac ggctccggct ttggctgact cgcagagata
     1741 agttacgccg tggctgtcca gattgcattc ccggatgcgt ttttccgcaa accgggtcag
     1801 ttgttcatag ctgagtgaaa acgtcatttc ctgatccatc ctgcacgttc catctgataa
     1861 tgttctgaca ggtaaaaatc a
//
LOCUS       DADMKF010000319         1877 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.324, whole genome shotgun sequence.
ACCESSION   DADMKF010000319 DADMKF010000000
VERSION     DADMKF010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1877
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.324"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(50..193)
                     /locus_tag="JNC24_004258"
                     /pseudo
     CDS             complement(50..193)
                     /locus_tag="JNC24_004258"
                     /inference="COORDINATES: protein motif:HMM:NF024729.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(197..1018)
                     /locus_tag="JNC24_004259"
     CDS             complement(197..1018)
                     /locus_tag="JNC24_004259"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAY9160831.1"
                     /translation="MFSHPVYLFLREFSLQDSRGGSLSKKAESLSSGKITIGTKEKVI
                     IINELRQCHPLSQLLVIADLPRSTFYYHVKRLNAPDPYQLVKQVILRIYHQHKGRYGY
                     RRIRLACRNESILLNGKTIRKLMKELGISSLIRRKKYRTYRGEQGRTCNNLLKRQFYA
                     DRPNQKWVTDVTEFKVDGRKLYLSPIMDLYNGEIVSYNLTERPLASMVKSMLLDAVEQ
                     LNKDDKPLLHSDQGWQYQMPRWQRWLSDNGITQSMSRRGNCLDNAAMESFFSTLK"
     gene            1007..1877
                     /locus_tag="JNC24_004260"
                     /pseudo
     CDS             1007..1877
                     /locus_tag="JNC24_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          469 a          415 c          455 g          538 t
ORIGIN      
        1 cgtaggtgcc caccttttca aatgatctgc gccccaaatc ttggacatcc tatgaaaaat
       61 gtttaagtcg atattgaacc ggactcatgc catcgagttt ctctttaatg cgatggtgat
      121 tgtagtattc aatgtaatca attatatctt gctttagatg atgtaactta ttgtatttat
      181 ttaggtagta acattcttat tttaaagtac taaaaaagct ttccattgct gcattatcta
      241 agcagttacc acgtcgggac atgctttgtg ttatgccatt atcactcaac caacgttgcc
      301 aacgaggcat ctgatattgc cacccctgat cactatgcaa caagggctta tcgtctttat
      361 tgagttgttc taccgcatct aatagcatgc tctttaccat tgatgctaat gggcgctcag
      421 taaggttata tgagactatt tctccattat acaggtccat gattggtgat aaatagagct
      481 tcctgccatc gaccttaaac tctgtgacat ctgttaccca cttttgattt gggcggtcag
      541 cataaaattg tctcttcagt aaattattac aagttcgccc ctgttctcca cgataggtac
      601 ggtacttctt gcgcctgata agtgagctga tacccagttc cttcattaac tttctgatgg
      661 ttttaccatt aagtaaaatg ctctcgttac ggcaggcaag tctgatgcgt cgataaccat
      721 aacgcccttt atgctgatgg tatattctca atattacttg tttaacaagt tgatatggat
      781 ctggcgcatt aaggcgtttc acgtgatagt aaaatgtact acgtggcaaa tccgcgataa
      841 ccagtaattg tgataatgga tgacattgcc ttaactcatt aataatgatt actttttctt
      901 ttgttccaat cgtaattttt cctgagctaa ggctttcagc ttttttagat aatgaaccgc
      961 cccgggaatc ctggagacta aactccctga gaaagaggta aacaggatga ctaaaaatac
     1021 tcgttttccc ccgaagtccg tcaacgggca gtccgtatgg ttctggaaag tcagggcgaa
     1081 tatgactcac aatgggcggt aatttgttcc attactccaa agattggctg tacaccggag
     1141 actctgcgtg tctgggttcg ccagcatgag cgggatacca ggggcggtga tggagggctc
     1201 accaccgctg aacgtcagcg tctgaaagag ctggagcgtg agaatcgtga actgcgctgc
     1261 agtaacgata tccttcgcca ggcttccgct tattttgcga aggcggagtt cgaccgcctc
     1321 tggaaaaaat aatgccactg ctggataagc tgcgtgggca gtacggggtc ggaccggtat
     1381 gcagtgaact gcatattgcc ccgtcaacgt attaccactg tcagcaacag gacatcatcc
     1441 tgataaacgc agtgcccgtg cgcagcacga tgactggctg aagagagaga tacagcgcgt
     1501 atacgatgaa aatcatcagg tgtacggtgt gcgtaaagtc tggcgccagt tgttacggga
     1561 aggaatcagg gtggccagat gtacagtggc gcgcctcatg gcggttatgg gacttgtcgg
     1621 tgttctccgg ggtaaaaagg tccgcactac cgtcagccgg aaaaccgttg ccgcaggtga
     1681 ccgcgtaaac cgtcagttcg tggcagaacg tcctgaccag ctgtgggtgg ctgattttac
     1741 ttacgtcagc acatggcagg gcttcgtcta tgtggcgttc atcattgatg tgtttgccgg
     1801 atacatcgtg tggtgacgag tctcatcgtc tatggaaacg acattcgtgc tggatgcact
     1861 ggagcaggcg ttgtggg
//
LOCUS       DADMKF010000320         1870 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.325, whole genome shotgun sequence.
ACCESSION   DADMKF010000320 DADMKF010000000
VERSION     DADMKF010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1870
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.325"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            81..1253
                     /locus_tag="JNC24_004261"
     CDS             81..1253
                     /locus_tag="JNC24_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS21 family transposase"
                     /protein_id="HAY9160832.1"
                     /translation="MLSREDFYMIKQMRQQGAYIVDIATQIGCSERTVRRYLKYPEPP
                     ARKTRHKMVKLKPFMDYIDMRLAENVWNSEVIFAEIKAMGYTGGRSMLRYYIQPKRKM
                     RPSKRTVRFETQPGYQLQHDWGEVEVEVAGQRCKVNFAVNTLGFSRSFHVFAAPKQDA
                     EHTYESLVRAFRYFGGCVKTVLVDNQKAAVLKNNNGKVVFNSGFLLLADHYNFLPRAC
                     RPRRARTKGKVERMVKYLKENFFVRYRRFDSFTHVNQQLEQWIADVADKRELRQFKET
                     PEQRFALEQEHLQPLPDTDFDTSYFDIRHVSWDSYIEVGGNRYSVPEALCGQPVSIRI
                     SLDDELRIYSNEKLVASHRLCSASSGWQTVPEHHAPLWQQVSQVEHRPLSAYEELL"
     gene            1253..>1870
                     /locus_tag="JNC24_004262"
     CDS             1253..>1870
                     /locus_tag="JNC24_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001775081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAY9160833.1"
                     /translation="MHELEVLLSRLKMEHLSYHVESLLEQAAKKELNYREFLCMALQQ
                     EWNGRHQRGMESRLKQARLPWVKTLEQFDFTFQPGIDRKVVRELAGLAFVERSENVIL
                     LGPPGVGKTHLAIALGVKAVDAGHRVLFMPLDRLIATLMKAKQENRLERQLQQLSYAR
                     VLILDEIGYLPMNREEASLFFRLLNRRYEKASIILTSNKGFADWGE"
BASE COUNT          473 a          462 c          533 g          402 t
ORIGIN      
        1 acggtaattc gccatttgga ttgtccgctc cacccaacat gttgtttcct taaggttctc
       61 acaccagaaa ggacatcaac atgctgagca gagaggactt ttacatgata aagcaaatgc
      121 gccagcaggg cgcgtacatc gtcgatattg cgactcagat tggttgctct gaacggacgg
      181 tcagacgcta cctcaaatac cctgaaccgc cagccagaaa gacccgccac aaaatggtta
      241 agctgaaacc gtttatggat tacatcgaca tgcgcctggc agagaatgtc tggaatagcg
      301 aggttatctt cgcggagatt aaggcaatgg gttatacggg cggacgttcc atgctgcgtt
      361 actacatcca gcccaaacgt aaaatgcgtc cgtcaaaaag aacagttcgc ttcgaaactc
      421 agcctggata ccagctccag catgactggg gcgaagttga ggtggaggtt gccgggcaac
      481 ggtgcaaagt taactttgcg gttaatacgc tggggttctc ccgcagcttc catgtcttcg
      541 ccgcaccaaa acaggatgct gagcatacct acgaatcact ggttcgcgcc ttccgctact
      601 tcggtggttg tgtgaaaacg gtgctggttg ataaccagaa ggctgcggtg ctgaagaata
      661 acaacgggaa agtcgtgttc aactccggat tcctgttgct ggctgaccac tataacttcc
      721 tgccacgggc atgccgtcca cgcagggcca gaacaaaagg taaggttgag cggatggtga
      781 aatacctcaa ggagaacttc ttcgtccggt accgcaggtt cgacagcttc actcatgtca
      841 atcaacaact ggagcaatgg atagccgatg tggctgacaa acgggagctt cgccagttca
      901 aagaaacgcc ggaacagcgc ttcgcgctgg agcaggaaca tctgcagccg ttaccggata
      961 cagacttcga taccagttac ttcgatatcc gccatgtgtc ctgggacagc tatatcgagg
     1021 ttggtggtaa tcgttacagc gttcccgaag cgctgtgtgg tcagccggta tcgatacgaa
     1081 tatcgctgga tgacgagttg cggatctaca gtaatgagaa actggtggcc tcacatcgcc
     1141 tctgttcagc atcgtctggc tggcagacag tgccggagca tcacgccccg ctctggcagc
     1201 aggtcagtca ggtggaacat cgaccactga gtgcctatga ggagctgttg tgatgcatga
     1261 actggaagtc ctgctgagtc gcctgaaaat ggagcatctg agttatcacg ttgaaagcct
     1321 gctggaacag gcagctaaaa aagagctgaa ctaccgggag ttcctgtgca tggcgctaca
     1381 gcaggaatgg aacggcaggc atcagcgcgg tatggagtcc aggctgaagc aggctcgtct
     1441 gccgtgggtc aaaacgctgg agcagttcga ctttaccttc cagccgggca tcgaccgtaa
     1501 ggttgtccgg gaactggctg gtctggcgtt cgtggagcgc agcgaaaacg tgatcctgct
     1561 gggccctcct ggtgtcggaa aaactcatct ggccatagct cttggcgtga aagcggtgga
     1621 tgcgggacat cgggtactgt ttatgccact ggacagactg atcgcgacac tgatgaaagc
     1681 gaaacaggaa aaccggctgg agcgtcagct acagcaactg agttatgccc gggtgttgat
     1741 cctggatgaa ataggctatc tgccgatgaa cagagaggaa gccagtctgt tcttccggct
     1801 actgaaccgt cgatatgaaa aagcgagcat catactgacg tcaaacaaag ggttcgcaga
     1861 ctggggagaa
//
LOCUS       DADMKF010000321         1836 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.326, whole genome shotgun sequence.
ACCESSION   DADMKF010000321 DADMKF010000000
VERSION     DADMKF010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1836
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.326"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            109..651
                     /locus_tag="JNC24_004263"
     CDS             109..651
                     /locus_tag="JNC24_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY9160834.1"
                     /translation="MKRSIIAAAVFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFE
                     AGGDSVSINMPTVPTTVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDK
                     ITGNDKAIASSNDTVGYYLYLGDNSDVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLT
                     DNSVQSGDVLSSLVMRVAQD"
     gene            731..1405
                     /locus_tag="JNC24_004264"
     CDS             731..1405
                     /locus_tag="JNC24_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HAY9160835.1"
                     /translation="MKGLLSLLIFSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLDIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNVKSNNANNWHLTIIDDHGN
                     YISDKI"
     gene            1421..>1836
                     /locus_tag="JNC24_004265"
     CDS             1421..>1836
                     /locus_tag="JNC24_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9160836.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSREVIKRLGINSDNFASGK
                     QCLTFEQLVQGGSYTWDIGVFRLDFSVPQAWVEELE"
BASE COUNT          584 a          325 c          402 g          525 t
ORIGIN      
        1 aataaaacaa tgtgtttaca ttgcggtaac ttttttgatg tgagaaattt gtttccaaga
       61 gatacagact cttaacaacc aaaaaaaaat tttattaagg aacatattat gaaacgttca
      121 attattgctg ccgctgtctt ttcttctttt tttatgagcg ctggagtatt tgctgcagac
      181 gttgataccg gaacattaac tattaagggg aatattgcag aatctccgtg taaattcgaa
      241 gcgggtggtg attcagtaag tattaatatg ccgactgtac caaccactgt ctttgaaggt
      301 aaagctaaat attctaccta tgatgatgca gtcggtgtaa ccagcagcat gttaaaaatt
      361 agctgcccga aagaagttgc tggtgtaaaa ctctcgttga ttaccaacga taaaataacc
      421 ggtaacgata aggcgatagc cagtagcaac gataccgtgg gttactatct ctatttaggt
      481 gataacagcg atgtcctgga tgtttctgca ccttttaaca ttgagagtta taaaacagcg
      541 gaaggtcaat atgctattcc gtttaaagca aaatacctga aactgacaga taactcagtg
      601 caatcaggtg atgtgttatc ttctctggtt atgcgtgtgg cgcaggatta ataaataaaa
      661 ttgtaagttt ctccaggtaa taaacatggc cgctatccca tggcggcctt ttaatttaag
      721 aggcattaaa atgaaaggat tattatcttt actcattttt tctatggtcc ttcctgcaca
      781 tgccggaatt gttatctacg ggacgcgcat tatttacccg gcagaaaata aagaagtgat
      841 ggtgcagttg atgaaccagg gaaaccgttc ttcgctgctg caggcgtgga ttgatgatgg
      901 cgatacgtca ttaccaccag aaaaaattca ggttcctttc atgttaacgc caccagtggc
      961 aaaaataggg gcaaattccg ggcagcaagt aaaaatcaaa attatgccga ataaactgcc
     1021 cactaataaa gaaagcattt tttatctgaa tgttctggat attccaccaa atagtccaga
     1081 gcaagaaggt aagaatgcac tgaagtttgc gatgcaaaac agaattaagt tgttttaccg
     1141 accagcgggt attgctccgg taaataaagc gacatttaaa aaattgctgg taaatcgcag
     1201 tggcaatggt ttggtgataa aaaatgactc agctaattgg gtgacgattt cggatgtcaa
     1261 agctaataat gtcaaagtca attatgaaac tattatgatt gcccccttag aaagtcagag
     1321 tgttaatgtc aaaagtaata atgcaaataa ctggcatctg accattatcg atgaccatgg
     1381 caactatatt agtgacaaaa tttaattgca ggagctgcct atgttgagaa tgaccccact
     1441 tgcatcagca atcgtagcgt tattgctcgg cattgaagct tatgcagctg aagaaacctt
     1501 tgatacccat tttatgatag gtggaatgaa agaccagcag gttgcaaata ttcgtcttga
     1561 tgataatcaa cccttaccgg ggcagtatga catcgatatt tatgtcaata agcaatggcg
     1621 cgggaaatat gagattattg ttaaagacaa cccgcaagaa acatgtttat caagagaagt
     1681 tatcaagcgg ttaggcatta atagcgataa cttcgccagc ggtaagcaat gtttaacatt
     1741 tgagcaactt gttcagggtg ggagctatac ctgggatatc ggggtttttc gtctcgattt
     1801 cagtgtcccg caggcctggg tggaagaact ggaaag
//
LOCUS       DADMKF010000322         1827 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.327, whole genome shotgun sequence.
ACCESSION   DADMKF010000322 DADMKF010000000
VERSION     DADMKF010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1827
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.327"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            114..665
                     /locus_tag="JNC24_004266"
     CDS             114..665
                     /locus_tag="JNC24_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HAY9160837.1"
                     /translation="MSAFKKSLLVAGVAMILSNNVFADEGHGIVKFKGEVISAPCSIK
                     PGDEDLTVNLGEVADTVLKSDQKSLAEPFTIHLQDCMLSQGGTTYSKAKVTFTTANTM
                     TGQTDLLKNTKETEIGGATGVGVRILDSQSGEVTLGTPVVITFNNTNSYQELNFKARM
                     ESPGKDATPGNVYAQADYKIAYE"
     gene            725..1827
                     /locus_tag="JNC24_004267"
                     /pseudo
     CDS             725..1827
                     /locus_tag="JNC24_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480735.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
BASE COUNT          588 a          370 c          352 g          517 t
ORIGIN      
        1 aaactaattt cataaaaccc gaaaagtaaa atataatatt tgaatgatta gcaatcctta
       61 aataacagtt actaatcatt cattttctac ttatatttat ataaaggaat attatgtctg
      121 cttttaaaaa gtcacttctt gttgctggcg tagcaatgat tctttccaat aacgtatttg
      181 ctgacgaagg tcacggcatt gttaaattta aaggtgaagt tatctctgct ccatgttcaa
      241 ttaaaccagg tgacgaagac ttaactgtaa atctgggtga agtagcagat accgttctga
      301 aaagcgacca gaaatctttg gctgagcctt tcactattca cctgcaagac tgcatgttga
      361 gccagggtgg aaccacttat tcaaaagcga aggttacttt caccacagcg aatactatga
      421 cgggtcagac tgacctgctg aaaaacacca aagaaactga aatcggtggt gccacaggcg
      481 ttggcgtacg tattctggat agccagagcg gcgaagtgac tttaggtact ccggtagtaa
      541 ttacattcaa caatactaac agctatcagg aactgaactt caaagctcgt atggaatccc
      601 cgggcaaaga tgctaccccg ggcaacgttt acgctcaggc tgactataag attgcttacg
      661 agtaatcaca ttaggccggg ttcgcccggc cacatcatta acggattaat gataagtgga
      721 acagatgtat aaaaaattaa aattaaccac aataagcgaa ttgattaaaa atatttattg
      781 ttcattatcc gttctcatca ttggttgtgc gtcagcttat gccgttgaat tcaacaaaga
      841 tttaatcgaa gccgaagatc gtgaaaacgt taacctttcc caatttgaaa ctgatggcca
      901 attacccgta ggtaaatact cattaaacgc tcttattaat aataaaagga cgccaatcca
      961 tctggacctg caatgggtat taattgataa ccaaactgca gtttgtctga ctccagagca
     1021 attaacatta ttaggcttta ctgatgagat tattgaagag gctcagcaaa acctgatcga
     1081 tggttgttac cctatcgaaa aagaaaaaca aattacaact tatctcgata aagggaaaat
     1141 gcaattatcc atatctgcac ctcaggcatg gttaaaatac aaagatgcaa actggacgcc
     1201 tcctgaactt tgggatcatg gtattgctgg ggcatttctt gactacaatt tatatgcctc
     1261 tcattatgca ccacatcagg gcgataattc acaaaatata agttcctatg ggcaggctgg
     1321 ggttaatctt ggggcctggc gcctgcgtac tgattaccag tacgatcagt catttaacaa
     1381 tggcaaaagc caggcgaaca acctggattt tccgcgtatt tatttgtttc gcccaatccc
     1441 agcaattaat gcaaaactaa ctataggtca atacgatact gaatcctcta ttttcgactc
     1501 tttccatttt tctggcgttt cgttgaaaag cgatgagaat atgttgccgc ctgatctacg
     1561 tggttacgca ccgcaaatca cgggtgttgc acaaacgaat gcaaaggtca ctgtctcaca
     1621 gaacaaccgt attatttatc aagaaaatgt tcctcctggc ccatttgcta ttactaattt
     1681 attcaataca ttacaggggc aactggacgt caaggttgaa gaagaggacg ggccagtcta
     1741 tatatcaatg acactaacca ttacatgaat tatcagaatg aaaaagagag ttatattgtc
     1801 acgtttaacc agtatcttga atcatta
//
LOCUS       DADMKF010000323         1818 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.328, whole genome shotgun sequence.
ACCESSION   DADMKF010000323 DADMKF010000000
VERSION     DADMKF010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1818
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.328"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(8..859)
                     /gene="hchA"
                     /locus_tag="JNC24_004268"
     CDS             complement(8..859)
                     /gene="hchA"
                     /locus_tag="JNC24_004268"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000218213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase HchA"
                     /protein_id="HAY9160838.1"
                     /translation="MTVQTSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMPHKDEKVMPFFEQHKSLFRNPKKLADVVASLNADSEYAAIFVPGGHGALIG
                     LPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHGDNPLNGYSICAFPDAADKQTP
                     EIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKVLTGDSPFAANALGKLAAQE
                     MLAAYAD"
     gene            1067..1310
                     /locus_tag="JNC24_004269"
                     /pseudo
     CDS             1067..1310
                     /locus_tag="JNC24_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001333883.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1450..>1818)
                     /locus_tag="JNC24_004270"
     CDS             complement(1450..>1818)
                     /locus_tag="JNC24_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460946.1"
                     /note="allows for ions and hydrophilic solutes to cross
                     the outer membrane; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HAY9160839.1"
                     /translation="AMMYAETRNMTPYGNVGIANKTQNFEAVAQYQFDFGLRPSLAYV
                     YSKGKDLGGNDYNNNGHQEYVDQDLVNYVEIGATYYFNKNFSTYVDYKINLLDKDDDF
                     YDNNGIATDDVVGVGLVYQF"
BASE COUNT          478 a          370 c          445 g          525 t
ORIGIN      
        1 acagggatta atccgcataa gctgccagca tttcctgcgc cgccagttta cccaacgcat
       61 tcgctgcgaa aggactgtcg ccggtgagaa ctttacggtc cttatgtact cgcccggtga
      121 tgtcgtcatt aataatgttc atgcccattt tcttcagctc ttcgccgaag taccaggtga
      181 gatgacccgg catatagcct atctctggcg tttgtttgtc tgcggcgtct gggaatgcgc
      241 aaatggaata accattcagt gggttatcgc cgtggcgaag cgccagaaaa gccgccgggc
      301 cgtggcaaag ggagataaca aaacggtcat ttttgattgc ccactgcaaa gcagcagcta
      361 cgtcctggct ttctggtaaa ccaataagtg cgccatgacc accaggaaca aagattgctg
      421 catattcgct atcagcgttg aggctggcga caacatccgc gagtttcttc ggattgcgga
      481 acaacgattt atgctgctca aagaatggca ttactttttc atctttgtgc ggcatagccc
      541 agtattcaaa cttggtcatc agaccggaaa tggtcgccac ttcgaattcg aaaccagcag
      601 catggagatg atacaacggc agcaacgttt caatcggatg gttaccggtc gagaacagtt
      661 ttccgttatc ggttggcaaa taacgttcgt ccgcggcgat caccagaatt ttatgcttac
      721 cgcgatacgg ttttggatag tccacgccat caagatcaga gacgggactg gtatattggc
      781 taagcgaata ttctgaaggg aagaatgcat tatcttcagc aatatcgacc tgcggatttt
      841 tacttgtttg aacagtcata gtgtattcct tattgttgct tagttagggt agtcactata
      901 tttgcgactg agctggtcgc tatttttaac cagtctggaa aattgagtca atatatcgaa
      961 agcggtagtc ggcgggtgta agtatgtaat gatgatgctg cactggtgtt gaatattatt
     1021 cacgccaaat tttccttatt gagcaacgtc gtcgcaagtg gcctcaatga ttttaggcca
     1081 ttatcaaagg tacggggcgg cggggagaga tttagtgctg tgaaatattg tggtactgat
     1141 gaatatttat atcgcggatt ggtagagaaa agattatttc atgcactatt tttgcaaata
     1201 tttctatatg ggaagcgtgt ttgttacgga ttaattcata aagaaatgca tttgggtgtt
     1261 ggtttgaaga gtcgctggtt gatgttattg tgccaggagg ggcagggtaa ggtgctgtat
     1321 cgatatgcat tggcaattgc gtgtgccgca gatggagatg aaatgccaat aaaaaagcct
     1381 gaattcgatg agggacgaac tcaggctagt gtttagtttt acagtaacac gccgcagcac
     1441 gttggctgat tagaactggt agactaagcc cacaccaaca acgtcatctg ttgcgatgcc
     1501 attgttgtca tagaaatcgt catctttatc caacaagttt attttatagt caacatatgt
     1561 tgagaagttc ttattgaaat agtaggtcgc tccaatttca acatagttga ccagatcctg
     1621 atcaacatac tcctgatgac cattgttgtt atagtcgtta ccgccgaggt ctttgccttt
     1681 tgaataaaca tatgcaagag atgggcgcag accaaaatcg aactgatact gtgctacagc
     1741 ttcaaagttc tgggttttat ttgcgatacc cacattacca tatggtgtca tattgcgggt
     1801 ttcagcatac atcattgc
//
LOCUS       DADMKF010000324         1768 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.329, whole genome shotgun sequence.
ACCESSION   DADMKF010000324 DADMKF010000000
VERSION     DADMKF010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1768
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.329"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(23..1708)
                     /locus_tag="JNC24_004271"
     CDS             complement(23..1708)
                     /locus_tag="JNC24_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HAY9160840.1"
                     /translation="MITIPITFYMLIAKYLCLLKPFWLRKNNKTSVLLIIIILAMILG
                     VVKIQVWLNDWNNDFFNALSQKETDKLWQLVLWFPALLGIFVLISVNKTWLIKLLTIR
                     WREWLTDYYLNRWFADKNYYFTQIYGEHKNTDNPDQRIAEDILLLISKTLSLSFGFIQ
                     SLSMLITFTVILWQSAGTLSFTVGGTEWNIQGYMVYTVVLIVIGGTLFTHKVGKRIRP
                     LNVEKQRSEATFRTNLVQHNKQTELIALSNAESLQRQELRDNFHTIKENWHRLMNRQR
                     WLDYWQNIYSRSLSVLPYFLLLPQFISGQINLGGLMKSRQAFMLVSNNLSWFIYKYDE
                     LAELAAVIDRLYEFHQLTEQRPTNKPKNCQHAVQVANASIRTPDNKIILENLNFHVSP
                     GKWLLLKGYSGAGKTTLLKTLSHCWPWFKGDISSPADSWYVSQTPLIKSGLLKEIICK
                     ALPLPVDDKSLSEVLHQVGLGKLAARIHDHDRWGDILSSGEKQRIALARLILRRPKWI
                     FLDETTSHLEEQEAIRLLRLVREKLPTSGVIMVTHQPGVWNLADDICDISTVL"
BASE COUNT          515 a          375 c          378 g          500 t
ORIGIN      
        1 tacagcaggg cttttattgt tgttataaaa ccgtgctaat gtcacaaata tcatcagcca
       61 ggttccagac accgggttga tgtgtaacca taatgacgcc gcttgtgggt agtttttcac
      121 gcactaaacg cagtaagcgt atagcctctt gttcctcaag atgagaggta gtttcgtcaa
      181 gaaatatcca tttcggacgt cgtaaaatta atcgtgccag ggcgatacgt tgtttttcgc
      241 cgctggaaag aatatctccc cagcgatcgt ggtcatgaat acgcgcagcc aatttcccca
      301 gaccaacctg atgcagtact tcgctcaacg atttatcgtc tacgggcagg ggaagtgctt
      361 tacaaataat ctctttcagt aagccgcttt tgattaacgg tgtttgtgac acataccaac
      421 tgtcagcagg agaagaaata tcacctttaa accacggcca gcagtgggat aatgttttaa
      481 gcagtgtggt ttttcccgca ccagagtagc ctttcagtaa tagccatttg cctggcgaaa
      541 catgaaagtt caagttctct aatatgatct tattatcagg cgtacgaata ctcgcattag
      601 ccacttgtac cgcatgttgg caatttttag gcttattcgt agggcgctgt tcagtgagtt
      661 gatggaactc atacaagcga tcgataaccg cagccagttc agcaagttcg tcatatttat
      721 aaataaacca gcttaaattg ttcgatacta gcataaatgc ctggcgcgat ttcatcagtc
      781 cgcccagatt gatctgcccg ctaataaact gcggcaataa cagaaagtag ggaagaacgc
      841 taagcgagcg cgaataaata ttttgccagt aatcaagcca ccgctgacga ttcattaaac
      901 gatgccagtt ctctttaatg gtatgaaaat tgtcgcgcaa ctcctggcgt tgtagagact
      961 cagcattgct tagcgcaatc agttctgtct gcttattatg ctgcacaaga ttagttcgaa
     1021 atgtcgcttc gctacgttgt ttttccacat taagcggacg aatacgttta cccaccttat
     1081 gggtaaataa agttccaccg atcacaatga gcacgacggt atagaccata tacccctgga
     1141 tattccattc tgttccacca acagtgaatg agagcgtacc cgcgctttgc caaagaataa
     1201 cggtaaaggt gatcagcatg ctaagtgact ggatgaagcc aaatgaaaga ctgagcgttt
     1261 tgctgatcag taagagaatg tcttcagcga tacgttggtc agggttatct gtatttttat
     1321 gctcgccgta gatctgcgtg aagtagtagt ttttatctgc aaaccagcga ttaaggtagt
     1381 aatctgttaa ccattcgcgc cagcgaatag tcagtagttt aattaaccat gttttgttta
     1441 cggatattaa tacaaagatc cccagcaggg cagggaacca aagaacaagc tgccaaagct
     1501 tatcggtttc tttctggctt agcgcattga aaaaatcatt attccagtcg tttaaccaga
     1561 cctgaatttt aaccacgcca aggatcattg cgaggataat aatgattaac aacactgaag
     1621 ttttattatt cttacgcagc caaaagggct tcagcagaca gagatacttt gctatcaaca
     1681 tataaaacgt aatgggaatg gttatcatta gcgaaaattg attgtgccat ccaatgatta
     1741 tctatggcaa tgatttactg atgaaatt
//
LOCUS       DADMKF010000325         1729 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.330, whole genome shotgun sequence.
ACCESSION   DADMKF010000325 DADMKF010000000
VERSION     DADMKF010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1729
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.330"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            79..1179
                     /gene="ompC"
                     /locus_tag="JNC24_004272"
     CDS             79..1179
                     /gene="ompC"
                     /locus_tag="JNC24_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HAY9160841.1"
                     /translation="MKLKIVAVVVTGLLAANVAHAAEVYNKDGNKLDLYGKVTALRYF
                     TDDKRDDGDKTYARLGFKGETQINDQMIGFGHWEYDFKGYNDEANGSRGNKTRLAYAG
                     LKISEFGSLDYGRNYGVGYDIGSWTDMLPEFGGDTWSQKDVFMTYRTTGLATYRNYDF
                     FGLIEGLNFAAQYQGKNERTDNSHLYGADYTRANGDGFGISSTYVYDGFGIGAVYTKS
                     DRTNAQERAAANPLNASGKNAELWATGIKYDANNIYFAANYAETLNMTTYGDGYISNK
                     AQSFEVVAQYQFDFGLRPSLAYLKSKGRDLGRYGDQDMIEYIDVGATYFFNKNMSTYV
                     DYKINLIDESDFTRAVDIRTDNIVATGITYQF"
     gene            1343..>1729
                     /locus_tag="JNC24_004273"
     CDS             1343..>1729
                     /locus_tag="JNC24_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HAY9160842.1"
                     /translation="MIMTNINTACVKNNASYQLNNALPNKETISSNFCERLEQWGNKS
                     VNNGEERAIAVERIKEAYNSNMASLDLSYLDLSELPPIPSTVNTLNLENNCLTCLDLA
                     DNASLVNINLSVNKINTITFPKESKLE"
BASE COUNT          524 a          350 c          358 g          497 t
ORIGIN      
        1 agtatgtcat ttataaatga cgccgtttaa aattcgttta gaaaatacgt ttaacgataa
       61 ttaaaaggat tctgttaaat gaaattaaaa atagttgcgg tggttgtaac tggtttgtta
      121 gctgcgaacg tagcacacgc tgccgaagtc tataacaagg atggtaataa actcgacctt
      181 tatggcaagg ttaccgctct acgttatttt actgatgata agcgtgacga tggtgataaa
      241 acttatgccc gtctcggctt taaaggagaa acgcaaatca atgatcaaat gattggtttt
      301 ggtcactggg aatatgattt taaaggctat aacgatgaag ccaacggctc gcgcggcaac
      361 aagacccgtc tggcctatgc tggtttaaaa attagtgaat ttggctctct ggactatggt
      421 cgtaactacg gtgtcggcta cgacattggt tcatggaccg atatgttgcc agaatttggt
      481 ggcgatacct ggagtcagaa agatgtcttc atgacatacc gtaccaccgg tctagcaacc
      541 tatcgcaact acgatttctt tggcttaatt gaagggctga actttgccgc gcaatatcaa
      601 ggcaaaaatg aacgtactga caacagtcat ctttatggtg ctgactacac gcgtgccaac
      661 ggtgacggtt tcggtatctc ctcaacttat gtttatgatg gctttggtat cggtgcggta
      721 tataccaaat ccgatcggac aaatgcgcag gaaagagccg ctgctaatcc tctcaatgcc
      781 tccggtaaga atgcagaact atgggccaca ggtataaagt atgatgccaa caacatctac
      841 tttgcagcta attacgctga aacattaaac atgaccacct atggggatgg ttatatctct
      901 aacaaagcac aaagttttga agtggtggcg caatatcaat tcgacttcgg cttgcgccca
      961 tcactcgctt acctgaaatc gaaaggcaga gatctgggcc gctacggcga ccaggacatg
     1021 attgagtata tcgacgttgg tgcgacgtat ttcttcaaca aaaatatgtc gacctatgtt
     1081 gattataaaa tcaacctgat tgatgaaagc gactttaccc gtgccgtaga tattcggacc
     1141 gataacatcg tcgctacggg cattacctat cagttctaac gtcctctcct gaccagccct
     1201 tctctgggct ggtttttgat ttaagtgctg ctgtatgact ccttaagtaa aaatgagcaa
     1261 tttttgatat ttctggtcat tcacctcaat taatattatt ccatatcctc cggggacatc
     1321 ttactataat atttattgat gagtgattat gaccaatatt aatacagctt gtgtaaaaaa
     1381 taatgccagt tatcaactta ataacgcatt acctaacaaa gaaaccatct ccagcaattt
     1441 ttgtgaacga ctagaacaat ggggtaataa atcggttaat aatggtgaag aaagagcaat
     1501 tgccgtggag cgaattaaag aagcttacaa ttcgaacatg gcatctcttg atttgtcata
     1561 tcttgatttg agtgaattac cgcctatccc ctcgacagtt aatacgttaa atctggaaaa
     1621 caattgtcta acttgtcttg acttagctga caatgccagt ctcgttaata tcaacctcag
     1681 cgttaataaa attaacacga taacctttcc aaaagaatca aaactggaa
//
LOCUS       DADMKF010000326         1705 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.331, whole genome shotgun sequence.
ACCESSION   DADMKF010000326 DADMKF010000000
VERSION     DADMKF010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1705
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.331"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            <1..1138
                     /locus_tag="JNC24_004274"
     CDS             <1..1138
                     /locus_tag="JNC24_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000508696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HAY9160843.1"
                     /translation="TWYGWDGDRLTTIQTGTTRIQTVYQPGSFTPLLRIETENGEQAK
                     ARHRSLAEVLQEDTGVTLPAELSVMLGRLERELRAGAVSAESEAWLAQCGLTAEQMAA
                     QLEAEYIPERKLHLYHCDHRGLPLALISPEGETAWQGEYDEWGNLLGETSAQHLQQSL
                     RLPGQQYDEESGLYYNRNRYYDPLQGRYITQDPIGLRGEWNLYKYPLNPVRFIDSLGL
                     KFHVNGDPSDFNQAVEYLKQDSQMKETIDFLSSSEETINIEYIEGTNVRFNSNNMTIY
                     WNSRASLFCSTELNSKSQSPALGLGHEFTHAQYCLLDKENFMALLSRTDKKYENKEEA
                     RVITIIESRAAKTLGECTRGAHSGLPFYRVDGPLQTMKITGTPE"
     gene            1150..1599
                     /locus_tag="JNC24_004275"
     CDS             1150..1599
                     /locus_tag="JNC24_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005141515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9160844.1"
                     /translation="MMKYLMVLLSLFSGSVLGMGRVNELCGIDSVKTIEIINLPSYVT
                     TLVPLSKEGLNEIYRYKVVVNEIRDLYAGKIIDLLQMKYFRKEKYNNIRWGGSIISKG
                     NNKCEIYFDAFGECGSVNGINVCFEKNEMIGWIKKEIPLLSQKIGGL"
BASE COUNT          513 a          281 c          489 g          422 t
ORIGIN      
        1 gacatggtac gggtgggacg gtgacaggct gacgacgata cagaccggca ccacgcgtat
       61 ccagacggta taccagccgg ggagcttcac gccgctcctg agaatcgaaa cagagaatgg
      121 cgaacaggcg aaggcgcggc accggagcct ggcggaggtg ttgcaggagg acacgggtgt
      181 gacgctaccg gcggagctgt cggtgatgct ggggaggctg gagcgggaac tgcgcgcggg
      241 agcggtgagt gcggagagtg aggcatggct tgcgcagtgc gggctgacgg cggagcagat
      301 ggccgcgcag ctggaggcgg aatacatccc ggagaggaaa cttcatcttt accactgcga
      361 ccaccgggga ctgccgctgg cgctcatcag cccggaaggg gaaacggcgt ggcaggggga
      421 gtatgacgaa tggggaaacc tgctgggcga aaccagcgcg cagcacctcc agcagtcact
      481 ccgtctgccg gggcagcagt atgatgagga gtcggggctg tactacaacc gcaaccggta
      541 ctatgacccg ttgcagggaa ggtatatcac ccaggatccg atagggctga gaggtgaatg
      601 gaatctgtat aaatatccat taaaccctgt tcggtttata gattctttgg gtttaaaatt
      661 ccatgttaac ggtgatccaa gtgattttaa tcaggctgtt gagtatttaa aacaagattc
      721 acaaatgaaa gaaactatag attttttatc aagctctgaa gaaactataa atatagagta
      781 tattgaagga accaatgtta gatttaattc gaataatatg acgatatatt ggaatagtag
      841 agcatcttta ttttgttcaa ctgaattaaa ctcgaaatca caatcgcctg cattaggttt
      901 gggacatgag tttactcatg cgcagtattg ccttcttgat aaggaaaatt tcatggctct
      961 tttaagccga acagataaga aatatgagaa taaagaagag gcaagggtta ttacaataat
     1021 tgaatcgcgt gcggcgaaaa ctttaggtga gtgtaccaga ggagctcata gtggactgcc
     1081 tttttatcgg gtggatggac cgttgcagac gatgaaaata acgggtacgc cagaatagtg
     1141 aggtttttta tgatgaaata tttgatggtt ttattgtctc tattttctgg ttcggtattg
     1201 gggatggggc gagtgaatga attgtgtgga atagatagcg tgaaaacgat agagataatt
     1261 aatcttccat cgtatgttac cacgttagtt ccgcttagca aggaaggtct taatgaaata
     1321 tacagatata aagtggtggt gaatgaaatt agagatcttt atgctggtaa gataattgat
     1381 ttattgcaaa tgaagtattt cagaaaagaa aaatataaca atataagatg ggggggtagt
     1441 atcatttcga aaggtaataa taaatgtgaa atttattttg atgcttttgg tgaatgtgga
     1501 agtgtgaatg gcattaacgt gtgttttgaa aaaaatgaaa tgataggttg gattaaaaaa
     1561 gaaatccctt tgctgtcgca aaaaataggt gggctttaaa tggtagggca atattcaaag
     1621 acagtatagg taactatttg tatggccatg taaggtaaaa gaaaaggaat aaaccatgag
     1681 cggaaaaccg gcggcgcggc agggc
//
LOCUS       DADMKF010000327         1677 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.332, whole genome shotgun sequence.
ACCESSION   DADMKF010000327 DADMKF010000000
VERSION     DADMKF010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1677
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.332"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(<1..889)
                     /locus_tag="JNC24_004276"
     CDS             complement(<1..889)
                     /locus_tag="JNC24_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY9160845.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFGILQTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYRCTFPPHPGRRLPVIKKTTSSTEHRCSIIDLASYTLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALFTVCLHP"
     gene            complement(989..1345)
                     /locus_tag="JNC24_004277"
     CDS             complement(989..1345)
                     /locus_tag="JNC24_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HAY9160846.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGKCAIP
                     DCVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQQVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(1481..1588)
                     /gene="rprA"
                     /locus_tag="JNC24_004278"
     ncRNA           complement(1481..1588)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="JNC24_004278"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
BASE COUNT          538 a          334 c          392 g          413 t
ORIGIN      
        1 tgggatgcag gcagacggta aacagagcaa taaatgagat aaaggtgtac agcatcagta
       61 atgttgttgg gcgaacggta ttacgaatga gtggagcggt aataaataca caaagcagcg
      121 aacccacggt gtagatactg agtagtttga tcgacatagt gtatgacatg cctgcaacaa
      181 attgtccgta ctgtgccagc cactggctaa ccagataaaa cgttgccatt gagatatagc
      241 catataaggt ataactggct aaatcgataa ttgaacagcg atgttccgta gagctggtgg
      301 ttttctttat gacaggtaag cgacgacccg gatggggtgg gaacgtacaa cggtataaaa
      361 acagagcgtt aataaacata atgcctgcag caatcataaa ggaccaaccg aaccacagtt
      421 cagcccacac taacaggcta atgattagcg gtaataaaaa ttgtccgctg gaaacaaatg
      481 ctttaattaa aatattggct gtcccaggtg agcgtggaaa agcttccatc aaactgggat
      541 aagtgcctgc atcgagaaaa ctgtttgcca ttcccgccag aaagccaaaa acataagcga
      601 taatgatgtt attggtctgc aggatgccaa aaaagaaggc catatagcag cacatcccga
      661 gcatgataaa agggcggcga ccaaagcgat cggataataa tcctgcaaaa agcaagacac
      721 ttaatcgacc aatgcccagc gatgagataa ctatcgagac acccgcggca ttagtctgcc
      781 aaagtgtctc cagcgaggcc atattcaggc tcatcaaaat gacgcccata ccatgcacca
      841 ggtaattaaa atacaaccca agtgcggtag ggtaataggg atttttcata ggttctactt
      901 agcaaaggtg ctttaaaaag tgtagcgatg ttgtgtcagt atccattcat tattgttgta
      961 acaacgacaa ttcctttctg tggaaaaatt aacaaagccg ctccagatcg tgtgcatata
     1021 attcagcggc taccgattga tagatcttcc agtcaataaa attgccatca gggtagactt
     1081 gttgaaacgc ggttaagtcg gggaaaaaac gcatggtgtt gttgccgatg cggttattta
     1141 ttttctctat gatggcatga agatgttttt ttcgttgctg acgcatagcc aacagttgct
     1201 cgactacaca atcaggaatg gcgcatttgc cattttccca ctgacgccag ctttcactgt
     1261 caccggtctg ggcaatccag gtcgcgcatt catcaatagt catggcaaaa atatggcgga
     1321 gtgcctggag ttcataagcg ttcatcgtgt aatggctgca tgaaataaac aataaaatat
     1381 aaataacatt atcagcctgc tgacggcttg aagagagtca cagtatcttg tgcaacatta
     1441 ttggaaagag tgaggggcga ggtagcgaag cggaaaaatg ttaaaaaaaa gcccatcgtg
     1501 ggagatgggc aaagactaca cacagcaatt cgttgtttca ctcaggggat ttccatgctt
     1561 ataaatcaat atgttgattt ataaccgtga gctaatagta ggcatacgga ctttttgcgt
     1621 cgatcagatt cgtctcatta gtttaaaaac tgtaaagaat ttgcgacagg tactacc
//
LOCUS       DADMKF010000328         1626 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.333, whole genome shotgun sequence.
ACCESSION   DADMKF010000328 DADMKF010000000
VERSION     DADMKF010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1626
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.333"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            57..1477
                     /gene="espX1"
                     /locus_tag="JNC24_004279"
                     /pseudo
     CDS             57..1477
                     /gene="espX1"
                     /locus_tag="JNC24_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308052.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspX1"
BASE COUNT          629 a          275 c          233 g          489 t
ORIGIN      
        1 gtcacattca ctcataaatt aaattaataa aaattcatca acttcaagga ctgataatga
       61 caaatggtat ctcaacttcg ccacattgtc tttataaatc aaatatagta gatgatgtaa
      121 taataaataa aacaagacaa aatgagttag taaaagtatt ttgcgagtat aaaacagagt
      181 tcttaatttt gtttgatgac ttttttagaa gccataactt gcctaaacca tctccagtcc
      241 ttcatcattt ttttcaatat actcatctga gagatgcata tttttataga tgtaaactca
      301 tagaacatac agttcaattt tattttttaa acacaaaggt atcacattac ttagattgga
      361 tgtttttgat gacagaacaa gcgaatgtct ttctgaagag attaagatat accaagaatg
      421 ccatgaaaaa ttcgttaatt ttttaaaggc aaacttcaac caagaaatat acccacagct
      481 atatactcct gaaatatttt atgaggcctg cagaaatctc cagtcatttt atgatcatca
      541 ggaaacgtca caaaacgcaa aatacagtgc aatagacaag aaaaaaagtc atcttaacca
      601 ggaaatcaaa aagcttatta aaaaaaatat tttccctgag ctatacaatg agcaatgcaa
      661 taaaatcccc tcctcatcga tagatgataa ccagggaata atgtggcaga actttaaaac
      721 tagcaattcg gcatattcaa aactatgtgg aaaattatct ttatttaaaa gcgtgcccag
      781 tcggttaata gaaaaatcag cttactgctc aactgaaaac atggccacta ataaatttaa
      841 tgtcgtcttt tcttactgcg gagataatat aaaggaattt atacttttat taccctataa
      901 taaatcactg gagatgtatg aacttaacga tcaaaagatc cagtatttga ctgctcttaa
      961 tattaatata cataaaatgt tgctatcaaa tataacaata gaaaagagca acctttcata
     1021 tgggtattat ttttgttgtg tattaaccaa tatatcatgt tttgaaagcg acttatcaaa
     1081 caccatcttt tcaaatggag aaataaacaa cctcttcatt aaaaaaagca acatatttgg
     1141 aacatcgttc actaatacca tgataaaaaa tttgcgctgt gaagatatta tgcctggccg
     1201 atggaccact caacttgtca ataagcactt aggttacaga tacacaggag tattcaaaac
     1261 cctggcaagt attgatgaca aaccgtcccg tttcgaaatt ttgataccat tggtacaaac
     1321 tttagttcgc gataatgtaa agctaaataa tgatgtgtat aaagaactta ataagtttat
     1381 gcacgattac gacaaaacat catcaaaaat gagaaaatac cttcagagca tcaacgagtg
     1441 catgctcctt ataaaaaata tagttcatca ggattaaaac actcaaagaa agaggtaatt
     1501 ttagaatgaa attatctgtg atgattatta atacactgca gagaaataaa aaacaatctg
     1561 aagcatgctc tgaaacatcc tactctgtca ctctaatgat ggcaccttgc tcttcttata
     1621 aagaag
//
LOCUS       DADMKF010000329         1621 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.334, whole genome shotgun sequence.
ACCESSION   DADMKF010000329 DADMKF010000000
VERSION     DADMKF010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1621
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.334"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            387..1469
                     /locus_tag="JNC24_004280"
     CDS             387..1469
                     /locus_tag="JNC24_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpD"
                     /protein_id="HAY9160847.1"
                     /translation="MKKLTVAISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKH
                     YFSSNDADDGDTTYARLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAF
                     AGLKFGDYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNN
                     DFFGLVDGLNFAAQYQGKNDRTDVTEANGDGFGFSTTYEYEGFGVGATYAKSDRTNNQ
                     VIYGNNSLNASGQNAEVWAAGLKYDANNIYLATTYSETQNMTVFGNNHIANKAQNFEV
                     VAQYQFDFGLRPSVAYLQSKGKDLGAWGDQDLVEYIDVGATYYFNKNMSTFVDYKINL
                     IDKSDFTKASGVATDDIVAVGMVYQF"
BASE COUNT          501 a          330 c          325 g          465 t
ORIGIN      
        1 ttttttacgt acgtaaacca ctaaatgata tttgcgagaa tgctaccgag tattgaaaac
       61 accactacaa atacataagc aaatcccaac aaataaccaa caaataattt ccagtgttat
      121 ttttagccgg tttaaattga accttcaaat tatagagcac ttataaataa cagccgttaa
      181 tataaattgg ctaatagatt tatttttatt cagtcaagag ccatgaatag gattcgatag
      241 aaaaaagttc agataaaaat agagatctac ttcacaaatc aaacgagaaa ccaaaactta
      301 catcttgaaa taatcacatt gattagatga atatttatcg cgcaatgaca tcatttttta
      361 ataatagttc aaaaaaaggg ctcacgatga aaaaattaac agtggcaatt tctgctgtag
      421 ctgcatcagt actgatggcg atgtctgctc aggcagctga aatttataat aaagacagta
      481 acaagctgga tctgtacggg aaagttaatg ctaagcacta cttctcctct aatgatgcag
      541 atgatggtga tactacttat gcccgtcttg gcttcaaagg tgaaacccaa atcaacgatc
      601 aactgactgg tttcggtcag tgggaatatg aattcaaagg caaccgcgct gaatctcaag
      661 gttcctccaa agataaaacc cgtcttgcct tcgctggcct gaaattcggt gactacggct
      721 ccatcgatta cggccgtaac tacggtgtag catacgacat cggtgcgtgg actgacgtcc
      781 tgccagaatt cggtggtgac acttggactc aaacagatgt gttcatgact ggtcgcacca
      841 ctggtgttgc aacttatcgt aacaacgact tctttggtct ggttgatggt ctgaactttg
      901 ctgctcagta tcagggtaaa aatgaccgca ctgacgtaac tgaagctaat ggtgatggtt
      961 tcggtttctc cactacttat gagtatgaag gattcggtgt aggtgcaacc tatgctaaat
     1021 ctgaccgcac taataatcag gttatctacg gtaacaacag cctgaatgca tctggtcaaa
     1081 atgctgaagt atgggcagct ggtctgaaat atgatgcgaa caacatctat ctggctacca
     1141 cctattctga aacccagaac atgactgttt ttggtaataa ccatattgcc aacaaagcac
     1201 aaaacttcga agtagttgca caatatcagt tcgacttcgg tctgcgtccg tccgttgctt
     1261 acctgcaatc taaaggaaaa gacttgggtg cgtggggtga tcaggacctg gttgaatata
     1321 ttgatgtagg tgcaacctat tacttcaaca aaaatatgtc cacttttgtt gattacaaaa
     1381 tcaacctgat tgataagagc gatttcacga aagcatctgg cgttgctacc gatgatatcg
     1441 ttgctgtagg tatggtttac cagttctaat ttgattacta aaagatatgt tgcgggaggc
     1501 tttgcctccc caacatataa gtggctccct caagccactt cctttaggag cacaaccttg
     1561 cttctaacta tataaacctt ctgttatata ttacccttta tttttggggg cgttgcaacg
     1621 c
//
LOCUS       DADMKF010000330         1593 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.335, whole genome shotgun sequence.
ACCESSION   DADMKF010000330 DADMKF010000000
VERSION     DADMKF010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1593
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.335"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            193..1236
                     /locus_tag="JNC24_004281"
     CDS             193..1236
                     /locus_tag="JNC24_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAY9160848.1"
                     /translation="MNDRLCFEVHDNKGYFVFPDTWFGPLLGEFEEVLDAYDADEISE
                     TSYINKLRRLAQREPDFIDIHAHLAYAFLEQNAPRKALNASLKGLAAGNRIIPESFCG
                     EIIWMHPENRPYLRALYAAILANVHLQRHQDAVMLTDKILAYNPEDNQGARWLLGSEL
                     LRTGDHERAFSVLKEHADEFSPYWYELGLLHFLNGEHVKAATAFRHGFATNTYIAEML
                     CGNLHPFPLAVWHDFSGSLDTAEDYYATYSPLWGQYPEALLFVNWLYNHSSVLHERSE
                     IIKCAEMLIQEDDFEICESILRQQEHLWKRIDKTLSEEIVQKCRNMNGEYIWPWILPF
                     SAAGMKHSSIQYQ"
BASE COUNT          456 a          355 c          337 g          445 t
ORIGIN      
        1 aatttagcat ttcaatcatc tgacatcatt ataaaaaatg agattcacat aaagataaaa
       61 ttattgttct caaggaaagc atgaaggggc ttcgtaagca atattcattc ggtgccaaga
      121 caggacttga tacacagaat ctcaactttt atgctctcag actatatcac cacatgtttt
      181 acaggctcag aaatgaacga tagattatgc tttgaagtgc acgataacaa gggttatttc
      241 gtatttccgg atacctggtt tggcccattg ctgggggagt ttgaagaagt gcttgatgca
      301 tacgacgctg atgaaatcag cgagacgagt tacatcaaca aactccgacg ccttgctcaa
      361 cgggagccgg actttattga tatacacgct catctggcat atgcatttct ggaacagaat
      421 gccccgcgta aggcactgaa cgcctctctt aaaggactgg cggcaggtaa tcgtatcatc
      481 ccggaaagtt tctgtggtga aataatctgg atgcatcccg aaaaccggcc ttatctgcgc
      541 gcgctttatg cagcaattct cgcaaatgtg cacttacagc gccatcagga tgcggttatg
      601 ctcactgata aaatactggc atacaatcct gaagataatc agggggctcg ctggttgttg
      661 ggttcagagc tgttacgcac cggcgatcat gagcgagcct tcagtgtact gaaagagcat
      721 gctgatgaat tttcacctta ctggtatgaa ctcggtctcc tgcattttct gaacggggaa
      781 catgttaagg ccgccacagc ttttcgccat ggctttgcca caaacactta catcgcagaa
      841 atgctgtgcg gaaatctgca tccatttcca cttgctgtgt ggcatgactt ctccggctct
      901 ctggatacgg cagaagatta ctatgcaaca tactcaccat tatggggaca atatccggaa
      961 gcattgctgt ttgtaaactg gctctataac cattcctccg tgctgcatga acgatcagaa
     1021 atcattaaat gcgccgaaat gctgattcag gaagatgatt tcgaaatatg cgaaagtatt
     1081 ttaagacagc aggagcacct gtggaaaaga attgacaaga cactttctga ggaaattgta
     1141 cagaaatgca gaaatatgaa cggtgaatat atctggccct ggatattgcc cttttccgca
     1201 gcagggatga aacattcaag catccagtat cagtagatat tgcattagtg taactttaaa
     1261 acaagtaata atttattatc caataataac actgtgttcc ttccccaggt gattaccctg
     1321 gggttatttt ctgaaagttt cctttgcaaa aggatagcgt tgtaaattac tttaacgaat
     1381 tactccatga actcattaaa gttctttcgt aatactttat cgtcaaacac gttatagctg
     1441 gcatcaaaaa ccacagattt accctgccag cgggtaatcc cctcctctgg cattttcggt
     1501 tgaactggta tccccgtgga tttgccccta tatttccaga catctgttat cacttaaccc
     1561 attacaagcc cgctgccgca gatattcccg tgg
//
LOCUS       DADMKF010000331         1564 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.336, whole genome shotgun sequence.
ACCESSION   DADMKF010000331 DADMKF010000000
VERSION     DADMKF010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1564
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.336"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            502..957
                     /gene="csgB"
                     /locus_tag="JNC24_004282"
     CDS             502..957
                     /gene="csgB"
                     /locus_tag="JNC24_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin minor subunit CsgB"
                     /protein_id="HAY9160849.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSTQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAIVVQRQSQMAIRVTQR"
     gene            998..1450
                     /gene="csgA"
                     /locus_tag="JNC24_004283"
     CDS             998..1450
                     /gene="csgA"
                     /locus_tag="JNC24_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin major subunit CsgA"
                     /protein_id="HAY9160850.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGNHGGGGNNSGPNSEL
                     NIYQYGGGNSALVLQTDARNSDLTITQHGGGNGADVGQGSDDSSIDLTQRGFGNSATL
                     DQWNGKNSEMTVKQFGGGNGAAVDQTASNSSVNVTQVGFGNNATAHQY"
BASE COUNT          474 a          302 c          357 g          431 t
ORIGIN      
        1 aatgatagaa aagttgtaca tttggttttt attgcacaat tttaaaaaat catacaaatg
       61 gtgataactt actaataatg catataaaaa atatttcggt gtagtccttt cgtcatgtaa
      121 aaagttgttg ttttttctcc acacctccgt ggacaaattt ttactccaaa aagacgaggt
      181 ttgtcacggc ttgtgcgcaa gacatatcgc agcaatcagc gatgggcaag aagaatgact
      241 gtctggtgat ttttgatagc ggaaacggag atttaaaaga aaacaaaata ttttttgcgt
      301 agataacagc gtatttacgt ggcttttaat actttggtat gaacaaaaaa agaaaaatac
      361 aacgcgcggg tgagttatta aaaatatttc cgcagacata ctttccatcg taacgcagcg
      421 ttaacaaaat acaggttgcg ttaacaacca agttgaaatg atttaatttc ttaaatgtac
      481 gaccaggtcc agggtgacaa catgaaaaac aaattgttat ttatgatgtt aacaatactg
      541 ggtgcgcctg ggattgcagc cgcagcaggt tatgatttag ctaattcaga atataacttc
      601 gcggtaaatg aattgagtaa gtcttcattt aatcaggcag ccataattgg tcaagctggg
      661 actaataata gtactcagtt acggcaggga ggctcaaaac ttttggcggt tgttgcgcaa
      721 gaaggtagta gcaaccgggc aaagattgac cagacaggag attataacct tgcatatatt
      781 gatcaggcgg gcagtgccaa cgatgccagt atttcgcaag gtgcttatgg taatactgcg
      841 atgattatcc agaaaggttc tggtaataaa gcaaatatta cacagtatgg tactcaaaaa
      901 acggcaattg tagtgcagag acagtcgcaa atggctattc gcgtgacaca acgttaattt
      961 ccattcgact tttaaatcaa tccgatgggg gttttacatg aaacttttaa aagtagcagc
     1021 aattgcagca atcgtattct ccggtagcgc tctggcaggt gttgttcctc agtacggcgg
     1081 cggtaaccac ggtggtggcg gtaataatag cggcccaaat tctgagctga acatttacca
     1141 gtacggtggc ggtaactctg cacttgttct gcaaactgat gcccgtaact ctgacttgac
     1201 tattacccag catggcggcg gtaatggtgc agatgttggt cagggctcgg atgacagctc
     1261 aatcgatctg acccaacgtg gcttcggtaa cagcgctact cttgatcagt ggaacggcaa
     1321 aaattctgaa atgacggtta aacagttcgg tggtggcaac ggtgctgcag ttgaccagac
     1381 tgcatctaac tcctccgtca acgtgactca ggttggcttt ggtaacaacg cgaccgctca
     1441 tcagtactaa tacatcattt gtattgtcag cgccagtgat ataagacggt aattcgccat
     1501 ttggattgtc cgctccaccc aacatgttgt ttccttaagg ttctcacacc agaaaggaca
     1561 tcaa
//
LOCUS       DADMKF010000332         1541 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.337, whole genome shotgun sequence.
ACCESSION   DADMKF010000332 DADMKF010000000
VERSION     DADMKF010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1541
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.337"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            <1..>1541
                     /locus_tag="JNC24_004284"
     CDS             <1..>1541
                     /locus_tag="JNC24_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HAY9160851.1"
                     /translation="LVLTTQAQRAEEARQQAISGGTEPSAFPDTLPGYTEYGRDNGIR
                     LSAVWLTHDPEYPENLPAAPLVRYGWTPRGELAAVYDRSNTQVRSFTYDDKYRGRMVA
                     HRHTGRPEIRYRYDSDGRVTEQLNPAGLSYTYQYEKDRITITDSLNRREVLHTQGEAG
                     LKRVVKKEHADGSVTQSQFDAVGRLRAQTDAAGRTTEYSPDVVTGLITRITTPDGRAS
                     AFYYNHHSQLTSATGPDGLEMRRKYDESGRLIQETAPDGDIIRYRYDNPHSDLPCATE
                     DATGSRKTMTWSRYGQLLSFTDCSGYVTRYDHDRFGQMTAVHREEGLSQYRAYDSRGQ
                     LIAVKDTQGHETRYEYNAAGDLTTVIAPDGSRNGTQYDAWGKAICTTQGGLTRSMEYD
                     AAGRVIRLTSENGSHTTFRYDVLDLLIQETGFDGRTQRYHHDLTGKLIRSEDEGLVTH
                     WHYDEADRLTHRTVNGETAERWQYDERGWLTDISHLSEGHRVTVHYGYDEKGRLTGER
                     QTVHHP"
BASE COUNT          366 a          454 c          478 g          243 t
ORIGIN      
        1 ctggtactga ccacgcaggc gcagcgggca gaagaagccc ggcagcaggc catttccggc
       61 gggacggaac cgtccgcttt tcctgatacc ctgccgggtt acaccgaata tggccgggac
      121 aacggcatcc gtctgtctgc cgtgtggctg acgcacgacc cggaataccc ggagaattta
      181 cctgccgcgc cgctggtgcg ctatggctgg acgccgcgcg gcgaactggc ggcagtgtat
      241 gaccgcagca atacacaggt gcgcagcttt acttacgatg ataaataccg gggccggatg
      301 gtggcgcacc gtcacacggg ccggccggaa atccgttacc gttacgacag cgacgggcgg
      361 gtgacagaac agctaaaccc ggcaggctta agctacacgt atcagtatga gaaggaccgc
      421 atcaccatca ccgacagcct gaaccgccgt gaagtgctgc acacgcaggg tgaagccggg
      481 ctgaagcggg tggtgaaaaa ggaacacgcg gacggcagcg tcacgcagag tcagtttgat
      541 gcggtgggca ggctcagggc acagacggat gccgcaggcc ggacgactga gtacagcccg
      601 gatgtggtga cgggactcat cacgcgcatc accacgccgg atggcagggc atcggcgttt
      661 tactataacc accacagcca gttaacgtca gccaccgggc ctgacgggct ggaaatgcgc
      721 cgcaaatatg atgaatccgg ccgtctgatt caggaaactg cccctgacgg cgatatcatc
      781 cgctaccgtt atgataatcc acacagtgac ttaccctgcg caacggaaga tgccaccggc
      841 agccggaaaa ccatgacgtg gagccgttac ggtcagttgc tgagcttcac cgactgttcc
      901 ggttatgtaa cccgttatga ccatgaccgc ttcgggcaga tgacggcggt gcaccgcgag
      961 gaagggctga gtcagtaccg cgcatacgac agccgtggac agttaattgc cgtgaaagac
     1021 acgcagggcc atgaaacgcg gtatgaatac aacgccgccg gtgacctgac caccgtcatt
     1081 gccccggacg gcagcagaaa cgggacacag tacgatgcgt ggggaaaagc tatctgtacc
     1141 acgcagggcg gtctgacgcg cagtatggaa tacgatgctg ccggacgggt catccgcctg
     1201 accagtgaaa acggcagcca caccaccttc cgttacgatg tactcgacct gctgatacag
     1261 gaaaccggct ttgacggccg cacacagcgt tatcaccacg acctgaccgg caaacttatc
     1321 cgcagcgagg atgaggggct ggtcacccac tggcactatg acgaagcaga ccgcctcacg
     1381 caccgcaccg tgaatggcga aaccgcagag cggtggcagt atgacgaacg cggctggctg
     1441 acagacatca gccatctcag cgaagggcac cgggtgacgg tgcattacgg gtatgatgag
     1501 aaaggccggc tgaccggtga gcgtcagacg gtgcatcacc c
//
LOCUS       DADMKF010000333         1471 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.338, whole genome shotgun sequence.
ACCESSION   DADMKF010000333 DADMKF010000000
VERSION     DADMKF010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1471
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.338"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            <1..122
                     /locus_tag="JNC24_004285"
     CDS             <1..122
                     /locus_tag="JNC24_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA helicase"
                     /protein_id="HAY9160852.1"
                     /translation="HFLPVDQESARVLSTPMKPGNPRRYSNKRTDSSKMERFF"
     gene            119..376
                     /locus_tag="JNC24_004286"
     CDS             119..376
                     /locus_tag="JNC24_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9160853.1"
                     /translation="MNNQTMTFTPEQLRKHAQEMLRHAEQLEKTGITKDAIRKDMVPA
                     LRELMQAKHRAQKAVDELVDCVAELETRVGKFEKLVQEVLR"
     gene            376..750
                     /locus_tag="JNC24_004287"
     CDS             376..750
                     /locus_tag="JNC24_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HAY9160854.1"
                     /translation="MRHEFILPYPPTVNTYWRRRDNTYFVSKAGERYRRDVALIVRQQ
                     RLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGVLMDDEQFDEINIVRGQ
                     PVSGGRLGVKIYPIMHEEQVKK"
     gene            747..>1471
                     /locus_tag="JNC24_004288"
     CDS             747..>1471
                     /locus_tag="JNC24_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HAY9160855.1"
                     /translation="MKLEDLPKYYSPKSPCLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRARATELLADYALSRCDRVAALRKLPAEIKPVVMRIMASY
                     AFEDYARSAASKKQCPCCYGEKFIESVVFTNKVQYPDGKPPVWAKCTKGVYPSYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHREESVKRGMPVIRDCQRCGGRGY
                     ERLPSTEAFNAICEVTNQITRAS"
BASE COUNT          406 a          311 c          424 g          330 t
ORIGIN      
        1 gacattttct gcctgtggac caggaaagtg cacgggtgct ttccactccc atgaagcccg
       61 gcaatccgcg cagatacagt aataaacgaa ctgacagcag taagatggag cgtttctttt
      121 gaacaaccag acaatgactt ttacccctga acaattgcgt aaacacgcgc aggaaatgct
      181 gcgtcatgct gaacaactcg aaaaaacggg cataacaaaa gacgctatcc gtaaagatat
      241 ggtacccgcg cttcgggaac tgatgcaggc gaagcatcgc gcacagaaag cagtagatga
      301 gctggtggac tgtgtggcag agctggaaac cagagtcgga aagtttgaaa aactggtgca
      361 ggaggtgctg cgctgatgcg tcatgagttt attttacctt atccgccgac ggtgaatact
      421 tactggcgac gtcgtgacaa cacatatttt gtatcaaaag ccggtgagcg ttatcgccgg
      481 gatgtggcgc ttattgtccg tcagcagcga ctgaaattaa gcctgtccgg aagactggcg
      541 ataaagatta ttgcagagcc accggataag cgccgtcgtg acctggacaa tatcctgaaa
      601 gcaccactgg atgcactgac acatgcggga gtgttaatgg acgatgagca gtttgatgaa
      661 atcaatatcg ttcgtggtca gccagtatct ggtggacgtc tgggggtgaa gatttacccc
      721 ataatgcatg aagagcaggt caaaaaatga aactggaaga tttaccgaaa tactactccc
      781 caaaatcccc ttgcctgacc gatgcatcgg cctcaacgtc aaaagatgcg ctgagtatca
      841 ctgatgtgat ggccgcgcag ggcatgacac agaatcgggc tgagatgggg ttttctgcgt
      901 tcctggggaa aatgggcatc agtatgaatg acagggcgcg ggcaacagaa ttactggcag
      961 attatgcact cagtcggtgc gatcgtgtgg cggcgttgag aaaacttccg gcagaaataa
     1021 aaccggtagt gatgcgcatt atggcttcgt acgcttttga ggattatgcc cgcagcgcag
     1081 cgagtaaaaa gcagtgccct tgttgctatg gggaaaaatt tattgaaagc gtagttttta
     1141 caaacaaggt ccagtatccg gatggtaagc cgccggtatg ggcaaagtgt acgaaaggtg
     1201 tgtatccgtc ttactgggaa gaatggaaaa aagtcaggga ggtggtaaaa gttgcctgtc
     1261 cggagtgtgg cggaaagggt gaggtttcca ccgcctgtaa ggattgccgt gggcgtggtg
     1321 tcgccattca tcgtgaagag tcggtaaaac gtggtatgcc tgttatcaga gactgccagc
     1381 gttgtggtgg tcgtggctat gaaagactac catcaacgga ggcatttaat gctatatgcg
     1441 aggtgacaaa ccagataaca cgcgcgtcat g
//
LOCUS       DADMKF010000334         1460 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.339, whole genome shotgun sequence.
ACCESSION   DADMKF010000334 DADMKF010000000
VERSION     DADMKF010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1460
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.339"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            271..1386
                     /locus_tag="JNC24_004289"
                     /pseudo
     CDS             271..1386
                     /locus_tag="JNC24_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312403.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          571 a          272 c          213 g          404 t
ORIGIN      
        1 aaaaaaatca cttcacacac tatcagagaa cataataact ttttaaaatg agcgcatttt
       61 gatagttgtt aacgatttcc tttacgatac tatcactacc ctttttttat acacaaatat
      121 taccgggtgg agataaaagg gaaatcaaag gtaattatat caggaagata taacgcatta
      181 catttattgt gtgtagaaga aaatccattc ctgttctaat tatccaattt aaacatctta
      241 gcataaaaca aatgatgaat aaggaatttt atgtcaattg actttacccc aggtataata
      301 aatacatatc acggcgatat ttataactgc acaacaaata ccgataatgc taaaacgcca
      361 gacacaccga agtggccttg cgataattgg gaagaacaac aacccattaa ttccacattc
      421 tctggagaag gatatatctc tgatcaatat gatttagtgc aacaccaact tcaacaaatt
      481 aatgcgtgcc acacgaacac tacatataca aatgcagact actcaaaagt tgtggctcaa
      541 cttgtaagtc ttattactaa cattgaaacg ataagttcga cacaacttac gcaacagaca
      601 caatcaatac ttaatcagat taataacatt agatatgaaa aaaacaaaag tgctgaatgt
      661 cgaatcattg ttgtcgctaa tcctaaaccc gacaaggcaa tcataactaa aattagcgta
      721 gaagagggaa tacccataac attcagtgtc caaaccatgt tttcagatac taattttatt
      781 gctgaacaac gagctgactt accaactaat ataaaagata ttcaaagcct gtatcaaaaa
      841 atgacgaagt tatatattga gcacagtgaa aataaaaaca ggatgaaagt ctttgccgga
      901 gccaacttta ttgatttcaa tatgacagga cagaacctgt ccgggttcgt tctaaccttg
      961 tcaaggtttt atttcgaaga tctacttaat ataaatttca ctgacgcaaa tttaggtgac
     1021 actatttttt acataaagaa caccctaccc ccaaattata taaagatgga caatatcttg
     1081 acaaacaaat cgaaggttta ttttcaacat tattaaccat taatgacaac tttttacgag
     1141 caaaagcgga aattgcttcg acaataatta aatttttaga ggccaggata accaatcttt
     1201 catacaatga catattaaaa tatcagcaag aattccaaaa acaatgttat aagcaagtta
     1261 aagcctttac gacactctct cgatacaata aaattcaaac atgggcagaa atgtctgaat
     1321 atcagttcga agtatttcaa tacgagacgc ttaaccccaa aaaaatgtca cacacgcctt
     1381 atttaaagag gccacttcct aatgaaaaag atataaatta tggggtggaa atagaaatac
     1441 catctggcaa aagaattcgc
//
LOCUS       DADMKF010000335         1459 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.340, whole genome shotgun sequence.
ACCESSION   DADMKF010000335 DADMKF010000000
VERSION     DADMKF010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1459
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.340"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(<1..1012)
                     /gene="cadA"
                     /locus_tag="JNC24_004290"
     CDS             complement(<1..1012)
                     /gene="cadA"
                     /locus_tag="JNC24_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase CadA"
                     /protein_id="HAY9160856.1"
                     /translation="MNVIAILNHMGVYFKEEPIRELHRALERLNFQIVYPNDRDDLLK
                     LIENNARLCGVIFDWDKYNLELCEEISKMNENLPLYAFANTYSTLDVSLNDLRLQISF
                     FEYALGAAEDIANKIKQTTDEYINTILPPLTKALFKYVREGKYTFCTPGHMGGTAFQK
                     SPVGSLFYDFFGPNTMKSDISISVSELGSLLDHSGPHKEAEQYIARVFNADRSYMVTN
                     GTSTANKIVGMYSAPAGSTILIDRNCHKSLTHLMMMSDVTPIYFRPTRNAYGILGGIP
                     QSEFQHATIAKRVKETPNATWPVHAVITNSTYDGLLYNTDFIKKTLDVKSIHFDSAWV
                     PYT"
     gene            complement(1092..1439)
                     /locus_tag="JNC24_004291"
     CDS             complement(1092..1439)
                     /locus_tag="JNC24_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000126034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HAY9160857.1"
                     /translation="MTALMILITLMNSAGGKASDLFGELTGIAVLLTMLPYFYSCVDL
                     IRFEGVNIRNFVSLICSVLGCVFCFIALMGASSFELAGTFIVSLIILMFYARKMHERQ
                     SHSMDNHTASNAH"
BASE COUNT          406 a          320 c          365 g          368 t
ORIGIN      
        1 tggtgtaagg cacccacgcg gagtcaaagt ggatggattt cacatccagt gttttcttga
       61 tgaagtcggt gttgtacagc agaccatcat aggtagagtt ggtaattaca gcatgtaccg
      121 gccaggttgc gtttggtgtt tctttcacgc gcttagcaat ggtagcgtgc tggaattcac
      181 tctgtgggat accaccaaga ataccgtaag cgttacgggt cgggcggaaa tagattggcg
      241 taacatcgct catcatcatc aggtgggtca gcgatttgtg gcagttacgg tcaatcagaa
      301 tggtgctgcc tgccggagca gagtacatgc caacaatttt gttcgcagtg gaagtaccgt
      361 tggtcaccat gtagctgcgg tctgcgttaa agacgcgagc gatatactgt tctgcttctt
      421 tgtgtggacc actatgatcc agcagagaac ccagttcaga tactgaaatg gaaatatcag
      481 atttcatggt attcggacca aagaaatcat agaacaggct acctaccggg cttttctgga
      541 atgcagtacc gcccatgtga ccaggagtac agaaagtata tttaccttca cgaacatatt
      601 taaacagtgc tttagtcagc ggaggcagaa tagtgttgat atattcgtca gtggtctgct
      661 tgatcttatt agcaatatct tcagcagcac ccagcgcata ttcaaagaag ctaatctgta
      721 aacgcaggtc attcaggctt acatcgagag tggaatacgt attagcgaac gcgtacaacg
      781 gcaggttctc gttcattttg ctaatttctt cgcacagctc gagattatat ttatcccagt
      841 caaaaataac gccgcacaga cgcgcattgt tttcgatcag ttttaataag tcgtcacggt
      901 cgttcgggta aacaatctgg aagttcagac gttcaagcgc gcgatgaagt tcacggatgg
      961 gttcttcttt aaaataaacc cccatgtgat tcaatattgc aataacgttc atagtcatat
     1021 ctccaggtaa aaaaggcccc tcccaacaca tgggacaaaa tgaaaggagg agcctcggaa
     1081 aatactttta attaatgtgc gttagacgcg gtgtggttat ccattgagtg gctctggcgc
     1141 tcgtgcattt tgcgagcgta gaacatcagg ataatcaggc tgacgatgaa ggtacctgcc
     1201 agctcgaagg agcttgcgcc catcagcgcg atgaagcaga acacacaacc cagtacagag
     1261 cagatcaggc tgacaaagtt gcggatgtta acgccttcaa aacgaatcag gtcaacgcaa
     1321 gagtagaagt acggcagcat agtcagcagt actgcgatac cggtcagttc accgaacagg
     1381 tcagatgctt taccaccggc agagttcatc agagtgatga ggatcatcag ggcagtcatt
     1441 ttcactgcag ccagcagca
//
LOCUS       DADMKF010000336         1435 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.341, whole genome shotgun sequence.
ACCESSION   DADMKF010000336 DADMKF010000000
VERSION     DADMKF010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1435
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.341"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            1..683
                     /locus_tag="JNC24_004292"
                     /pseudo
     CDS             1..683
                     /locus_tag="JNC24_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            848..>1435
                     /locus_tag="JNC24_004293"
     CDS             848..>1435
                     /locus_tag="JNC24_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708747.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HAY9160858.1"
                     /translation="MNKVYSLKYCPVTGGLIAVSELARRVIKKTCRRLTHILLAGIPA
                     ICLCYSQISLAGIVRSDIAYQIYRDFAENKGLFVPGANDIPVYDKDGKLVGRLGKAPM
                     ADFSSVSSNGVATLVSPQYIVSVKHNGGYRSVSFGNGKNTYSLVDRNNHPSIDFHAPR
                     LNKLVTEVIPSAVTSEGTKANAYKYTERYTAFYRVG"
BASE COUNT          375 a          325 c          365 g          370 t
ORIGIN      
        1 aagtctggcg tcagttgtta cgggaaggta tcagagtggc cagatgcact gtggcacgtc
       61 tcatggcggt tatgggactt gccggtgttc tccggggtaa aaaggtccgt acgaccatca
      121 gccggaaagc cgttgccgca ggcgaccgcg taaaccgtca gttcgtggca gaacgacctg
      181 accagctgtg ggtggctgat tttacttacg tcagcacatg gcagggcttc gtctatgtgg
      241 cgttcatcat tgatgtgttt gccggataca tcgtgtggtg acgagtctca tcgtctatgg
      301 aaacgacatt cgtgctggat gcactggagc aggcgttgtg ggcccgtcgc ccgtctggca
      361 ccatccatca cagcgataaa ggctctcagt atgtgtcact ggcctataag gcgcgactaa
      421 aagaagcaaa actgctggca tcaacaggga gtactggtga ctcgtatgac aacgcgatgg
      481 cagagatcat caagggcctt tacaaagcgg aggtaataca ccgtaagagc tggaaaaacc
      541 ggacagaagt ggagctggcc acactcacgt gggtggactg gtataacaat cgacgattgc
      601 tggaaaggct gggccatatc ccaccggcag aagcagaaaa agcttattat gcttccatcg
      661 gaaatgatga tctggcagcc tgagttcaca gataaaacac tctccaggaa acccggggcg
      721 gttcagttca caaaaacaca ttaatgcagt aactatattt tcctttctgg tgataacgtc
      781 gggttatcat tagcttcttc agctatttta cttttatatc ccttgtaaac atcatggaga
      841 atccatagtg aataaagttt attctcttaa atattgcccc gtcaccgggg ggcttattgc
      901 tgtctctgaa cttgcccgca gggtaataaa aaagacatgc cgaagattaa cgcatattct
      961 tctggctggc attccagcaa tctgtctgtg ttactctcag atatccctgg cgggtattgt
     1021 ccgttccgat attgcctatc agatttatcg tgatttcgcc gaaaacaaag ggctttttgt
     1081 acctggtgcc aatgatattc cggtatatga taaggacgga aaacttgtgg gaagactggg
     1141 taaagcccca atggccgatt tcagcagtgt gagctcaaat ggcgttgcta cgcttgtatc
     1201 gcctcagtat atcgtcagcg taaagcataa cggaggatat cggagtgtga gctttggtaa
     1261 tgggaaaaat acatattccc ttgttgaccg taataaccac ccttctattg acttccatgc
     1321 tccacgtctg aataaactgg ttacagaagt tattccctca gcggtaacat cagaaggaac
     1381 caaagccaat gcttataaat acactgaacg ttacaccgct ttttatcggg tgggt
//
LOCUS       DADMKF010000337         1430 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.342, whole genome shotgun sequence.
ACCESSION   DADMKF010000337 DADMKF010000000
VERSION     DADMKF010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1430
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.342"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            22..1104
                     /locus_tag="JNC24_004294"
     CDS             22..1104
                     /locus_tag="JNC24_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005099906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HAY9160859.1"
                     /translation="MFRIPVNQWTLATAALGGVFLVSGLILLMNYNHPYTFTAQKAVI
                     AIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQARVDRLQADLMTATHNIKTL
                     RAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFSERDIDDARQNFLAQDALV
                     KGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLEQTVIRAPSNGYVTQVLIR
                     PGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGDDAEVVFNALPGQVFHGKL
                     TSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPNDDIDALPDGIYAQVAVYSD
                     HFSHVSVMRKVLLRMTSWMHYLYLDH"
BASE COUNT          346 a          327 c          383 g          374 t
ORIGIN      
        1 ctggcgtggg cggtctttaa aatctttcgt attccggtga atcagtggac gctggcgacg
       61 gcggcgctgg gaggcgtgtt tctggtgagt ggtttgattt tgttgatgaa ctacaaccac
      121 ccttacactt ttaccgcgca aaaggcagtg atagcgatcc ctatcacgcc acaggtgacg
      181 ggaattgtta ctgaagtcac tgacaagaat aatcagctta ttcaaaaggg cgaggtgctt
      241 tttaagctcg acccggttcg ttaccaggcg cgagttgaca gacttcaggc tgacctgatg
      301 acggcgacgc ataatataaa gacgctgcgt gcgcagctca ctgaagcgca ggccaacacc
      361 acccaggttt cagcggagcg cgaccgtctg tttaaaaatt atcaacgtta cctgaaaggc
      421 agccaggcgg cggtgaatcc gttctcggaa cgtgacatcg acgatgcgcg gcaaaatttc
      481 ctcgcgcagg atgcgctggt gaaaggctcg gtggcggagc aggcgcagat ccagagccag
      541 ctcgacagta tggttaacgg cgagcaatcg cagattgtga gcttaagagc gcaacttact
      601 gaagcaaaat ataaccttga gcagactgtc attcgcgcgc cgagcaatgg ctacgttact
      661 caggtactga tccgcccagg tacatacgca gctgccttgc cgctgcgtcc ggtgatggtc
      721 ttcatccccg agcaaaaacg gcaaattgtc gcccaatttc ggcaaaactc gctgttacgt
      781 ctgaaacctg gcgatgatgc ggaagtggtg tttaacgcgc tacctgggca ggtgttccac
      841 ggcaaattga ctagtatttt acctgtcgtg ccaggcggtt cttatcaggc gcagggggta
      901 ttgcaatcat taacggtcgt tcccggcacg gacggcgtgc tgggaaccat tgaactggac
      961 cctaacgatg atatcgatgc cttacccgac ggcatttacg cccaggtagc agtttactcc
     1021 gaccatttca gccatgtttc ggtgatgcgg aaggtgctgc taagaatgac cagttggatg
     1081 cattatttgt atttggatca ttgaggggga agggggagat tatggaactt tgctccctgc
     1141 aagggctaat tatttttgca tattacttca ttggctgcga tgatttttct ttaatattca
     1201 ttatgttaat aagttctgga tgagtgtgct ctcattggat tttttatccg tcttgttctg
     1261 ccgcaggatg agactataca tcgtcgagat cgcaatgatg atatgagttg ttcctcctgc
     1321 gacggctcta atcaaaaaag agcaggagtg cttttccatc tattgcgatg atggctttat
     1381 tatctgaagg atgacagtca catttaaaaa atatttaatc tatagatcac
//
LOCUS       DADMKF010000338         1430 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.343, whole genome shotgun sequence.
ACCESSION   DADMKF010000338 DADMKF010000000
VERSION     DADMKF010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1430
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.343"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            13..438
                     /locus_tag="JNC24_004295"
     CDS             13..438
                     /locus_tag="JNC24_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000056357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HAY9160860.1"
                     /translation="MLRIIHTKGVSLPDDRESVFWLNIKNIPPSASNKATNSLEIAVK
                     TRIKLFWRPANIRLIPEDAAPKVKWRREGRNLIAENPNPIHISVMDVIVDGHDVPLNM
                     IRPFETLTLPLPANSAGGQMTWRFINDYGAVSDPIKMTL"
     gene            463..>1430
                     /locus_tag="JNC24_004296"
     CDS             463..>1430
                     /locus_tag="JNC24_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HAY9160861.1"
                     /translation="MMTTRIVVGLTAGTCLIFSQNLMAEVSVFNPALLEIDHQSGVDI
                     RQFNRANLMPPGVYSVDIFINGKMFERQDVTFVQDNPDADLHACFIAIKKTLSSFGIK
                     VDALKSFNDVDETVCLDPAPRIEGSSWQFDSDKLQLNISIPQIYMDAMAYDYISPTRW
                     DEGINALTINYDFSGSHTLRSDYGSQETDTSYLNLRNGLNIGPWRLRNYSTLNTSDGR
                     AEYNSISTWIQRDIAALRSQIMIGDTWTASDIFDSTQIRGARLYTDNDMLPASQNGFA
                     PVVRGIAKSNATVIIRQNGYVIYQSAVPQGAFEITDLNTASTGGDL"
BASE COUNT          386 a          331 c          341 g          372 t
ORIGIN      
        1 tcgcaagaaa aaatattacg cattattcac accaaaggtg tgtcattgcc tgatgatcgc
       61 gagtcagtct tctggctgaa tattaaaaat attccgccat cagcgagcaa caaagcgacg
      121 aactcgctgg aaatagcagt aaaaacgcgt atcaagctgt tctggcgacc agcaaatatc
      181 cgccttattc ccgaagatgc tgcgccgaaa gtgaaatggc gccgggaagg gcgaaatctg
      241 atagcagaga atcccaaccc aattcatatc agtgtgatgg atgtcatcgt tgatggccat
      301 gatgtgcccc tcaatatgat ccgtcctttt gaaacgctca cgttaccttt acctgctaat
      361 tctgcgggag gccaaatgac ctggcgattt attaatgatt acggcgcagt cagtgacccg
      421 ataaaaatga cgctgtaagc aagggataag gataggcgta ttatgatgac gaccagaata
      481 gtggttggcc tcacggcagg gacgtgtctg attttctcgc aaaacctgat ggccgaggtc
      541 agtgtattca atccggcgct tctggaaatc gaccatcaat ccggagtcga tattcgccag
      601 tttaatcggg caaacctgat gcccccaggt gtttatagcg ttgatatttt tatcaacggt
      661 aaaatgtttg aacgtcagga tgtgacattt gttcaggata atccagatgc tgatctgcac
      721 gcttgcttta ttgccattaa aaaaacactg tcctcctttg gcataaaagt tgatgcgctc
      781 aaatcgttca atgatgtgga tgagacggtt tgcctcgatc ctgctccacg tattgaaggc
      841 tcatcctggc agtttgacag tgataaattg cagctgaata tatccattcc ccaaatctac
      901 atggacgcga tggcttatga ttacatcagc cccacgcgtt gggatgaggg gattaacgcg
      961 ctcaccatca actacgattt ttctggttca catacactac gttcagatta tggttcacaa
     1021 gagacagata ccagttatct caatctgcgc aatggactga atattggacc gtggcggcta
     1081 cgtaattaca gtactttaaa caccagcgat ggccgtgcgg aatacaactc cattagtacc
     1141 tggatacagc gcgatattgc cgcgttaaga agccagatta tgattggtga tacgtggacg
     1201 gcgagcgata ttttcgacag tacgcaaatt cgcggcgcgc gtttgtatac tgataacgat
     1261 atgctacccg ccagccagaa tggctttgct cctgtggttc gtgggattgc aaagtccaac
     1321 gccaccgtca tcattcggca gaatggctac gtgatttatc agtctgccgt tccacaaggt
     1381 gcttttgaga tcaccgatct caacaccgcc agtacaggtg gcgatttgga
//
LOCUS       DADMKF010000339         1426 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.344, whole genome shotgun sequence.
ACCESSION   DADMKF010000339 DADMKF010000000
VERSION     DADMKF010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1426
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.344"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(130..822)
                     /gene="rutB"
                     /locus_tag="JNC24_004297"
     CDS             complement(130..822)
                     /gene="rutB"
                     /locus_tag="JNC24_004297"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HAY9160862.1"
                     /translation="MTTLTARPEAITFDPQQSALIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPDDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEYFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            complement(822..>1426)
                     /locus_tag="JNC24_004298"
     CDS             complement(822..>1426)
                     /locus_tag="JNC24_004298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HAY9160863.1"
                     /translation="GDFFTMNDCRVSPQPSVPMKVICAGQSDAGMAFSAQYADFNFCF
                     GKGVNTPTAFAPTAVRMKQAAEQTGRDVGSYVLFMVIADETDDAARAKWEHYKAGADE
                     EALSWLTEQSQKDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPG
                     AEGVLLTFDDFLSGIETFGERIQPLMQCRAHLPALTQEVA"
BASE COUNT          323 a          396 c          396 g          311 t
ORIGIN      
        1 tatgttgatc aaaagccagc gtaccggaga catacaccac gccatcagcc agcgtgccgg
       61 gaacgaaggg ggccagcggt gcgctgctgc cagcgggaat aattacggat tttggcatcg
      121 ttaaactcct taagcgatac gagcaaagga cgtgggagaa agcgcgtcgc agaacgtttc
      181 gacgtcgctg acccagccaa aaaaggtttc gatattgaac aacgcggctt tctgtgcaaa
      241 ttcaggcccc gcctggtgtg ttgcgtcttc cagcaccacg ccgaaatact ccagaaaaaa
      301 gccatcgcgt agcgtcgatt cgacgcagac gttggtagcg ataccggtga aaaccagatg
      361 gcgtattcca cggctgcgca aaatgctgtc cagcggcgta ttgaagaaac cgctgtagcg
      421 cggcttcggc agcacaatat cgtcaggctg cggcaccagt tcatccacca gttgataatc
      481 ccaggagcct ttcgccagca atttcccctg cagctgcggc tgcttacgca tggttttcag
      541 ggcgttcgat ttatgaaaat tcggtgagcc gggtccgcca gcttcgacat actgttcatc
      601 ccagccattt tgaaaccaga tgatcagcat ccctgccgct cgcgctgcgg tcacggcggt
      661 ttgaatgttg gcaatgaccg ggcgagtggt tgagacatca aacccggcga gatctaagta
      721 gccgcctggc gtggcataag cgttttgcat atccaccacg atcagcgcac tttgctgcgg
      781 atcgaaggta atggcttccg gtcgagcggt taaggtcgtc atcatgccac ctcctgagtc
      841 agcgcaggga gatgggcgcg gcactgcatc agtggttgaa tgcgctcgcc gaaggtttcg
      901 attcccgaca gaaaatcatc gaaggtcaac agcacgcctt cggcaccagg cacgcttgcg
      961 acttcatcta acatgcgcgc gacactggcg taagaaccga ctaatgtccc catattgatg
     1021 tttaccgccg aagtgggatc ggccatctga cggacgttgg tgtcagtacc ggagcgggta
     1081 tctttctgac tttgttcggt tagccagctt aacgcctctt catccgcgcc cgctttgtag
     1141 tgttcccatt tggcgcgagc ggcatcgtcg gtttcatcgg caatcaccat aaacaacaca
     1201 taagagccaa cgtcgcgccc ggtttgctct gcggcctgtt tcatccgcac agcggtcggg
     1261 gcgaaagccg tgggtgtatt tacgcctttg ccgaaacaga agttgaaatc ggcatactga
     1321 gcggagaacg ccatgccagc gtcgctttgc ccggcgcaga tcactttcat ggggacactc
     1381 ggttgcggac tgacgcgaca atcattcatg gtgaaaaaat cgcctt
//
LOCUS       DADMKF010000340         1419 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.345, whole genome shotgun sequence.
ACCESSION   DADMKF010000340 DADMKF010000000
VERSION     DADMKF010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1419
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.345"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(<1..651)
                     /locus_tag="JNC24_004299"
     CDS             complement(<1..651)
                     /locus_tag="JNC24_004299"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415792.1"
                     /note="Catalyzes the conversion of citrate to isocitrate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HAY9160864.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTM"
     gene            complement(815..910)
                     /locus_tag="JNC24_004300"
     CDS             complement(815..910)
                     /locus_tag="JNC24_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9160865.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            complement(1024..1191)
                     /locus_tag="JNC24_004301"
     CDS             complement(1024..1191)
                     /locus_tag="JNC24_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9160866.1"
                     /translation="MVGQEQLESSPLCQHSDNETETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            complement(1194..1358)
                     /locus_tag="JNC24_004302"
     CDS             complement(1194..1358)
                     /locus_tag="JNC24_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HAY9160867.1"
                     /translation="MLISDGDYMRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVV
                     VALLIWKVWG"
BASE COUNT          378 a          348 c          330 g          363 t
ORIGIN      
        1 catagtggtg tgcgagtcag taccaacgag tgtatccgga taagcaatcc attcaccgtc
       61 ctgcaattca ctccacactg ctttgccgag atattcgagg ttaacctgat ggcaaatgcc
      121 tgtgcctggc ggcacgacgc taaaccgact gaacgcttgc tttccccatt tcaggaacac
      181 ataacgttcg tggttgcgct ccatttccag gcgtacgttt tcttcaaatg cctcatcatc
      241 accaaaacga tcgacggtca ccgagtggtc aatgaccagg tcgaccggtg agagcgggtt
      301 aacctttgca gtatcgccgc cgaggcgttt aaccgcttcg cgcattgccg ccagatcaac
      361 aacggcaggt acgccggtaa agtcctgcat caacaccctt gccgggcggt aggcaatttc
      421 acggtcagca tgggcatttt tcagccatcc tgccagcgcg tggatatcct cttcggtaac
      481 cgagttacca tcctgccagc gcagcaggtt ttcgagcaaa actttgagtg acttgggtag
      541 acgggtgata tcgcccagtg atttagcagc aagcggcagg ctgtagtagt ggtaagtttt
      601 atctttggcc tgcaacgtgt ccttactggc ttctcgtagg gttgacgaca tagctcctcc
      661 ttaatgacag ggttgcgaag ccctgattct cttcagggtg aatattaaag ataacacaaa
      721 catcgcgtaa cgatttgata acaacccaaa ttgataaaag aggagaaacc ttgtggcaaa
      781 gcaaatgaca accccgccgc agcggggtca aggatcagtg agtaatgatc cagaggagtt
      841 cggcccagaa gagcatcgac agagaaaacg cgcccatcca ggaccaatat ttcatatttg
      901 tgttgatcat aggctacacc cttaacggtt attattttgt gttgagataa ctcaagctgt
      961 tattgctgcg gtgtattaaa agccctttaa ttcattaaaa atgggtaata agaatgatga
     1021 taattattgt ttcggctgat catcttcagc aaacagttct ataaaggctt gctgctgcga
     1081 tgttagttgc cagtcgtctg gaataacgac ggaacattcc cgtttcgttt ctgtttcatt
     1141 gtcactatgc tggcataatg gtgacgactc cagttgctcc tgaccgacca tagttatccc
     1201 cacactttcc aaatcagcag tgcgacaacc acccagaaaa gtgcagaacc aagaaaaacc
     1261 gccagccagg ctttacgttt taattcagga tcgcggcggg gttcctgatt tccagaaggc
     1321 attgctaacc tcatataatc gccatcactt atcagcaaga cgttgaacga ttggtgtaat
     1381 taatcatacg atgagacaaa tcctaaagaa actttagtc
//
LOCUS       DADMKF010000341         1393 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.346, whole genome shotgun sequence.
ACCESSION   DADMKF010000341 DADMKF010000000
VERSION     DADMKF010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1393
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.346"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            <1..460
                     /locus_tag="JNC24_004303"
     CDS             <1..460
                     /locus_tag="JNC24_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAY9160868.1"
                     /translation="LNAMASQRTKEARGGELQINELLPASLDAAKAHGLTERDVYEAT
                     DYYKTPRGQQPGGATKMLFSHAQKTLAWDAFAFTEVLLTQPVMVVVGEKVGAFGAYRD
                     GLEVYGRAMVSQDRQLVSLPDFSHYELYDKPEAVQEALEKVIPFFNTHLG"
     gene            478..1116
                     /locus_tag="JNC24_004304"
     CDS             478..1116
                     /locus_tag="JNC24_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAY9160869.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSKIIMGDVLNHAALKQAMQGQDVVYANLTGEDLDIQANSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFGEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSRKSVAALITDIIDKPEKHIGENIGINQPVTDGDKPLFM"
BASE COUNT          445 a          282 c          307 g          359 t
ORIGIN      
        1 attgaatgca atggcgtcgc aacgaaccaa agaggctcgt ggtggagaat tacagatcaa
       61 cgaattactt cccgccagcc tggatgcagc aaaagcccat ggactgaccg aacgcgatgt
      121 ttatgaagca actgactact ataaaacacc acgcgggcaa caaccgggag gtgcgacaaa
      181 aatgttgttc tcacatgcac agaaaacgct ggcctgggat gccttcgcat ttactgaagt
      241 gttactgact cagccggtga tggtagtagt tggtgaaaaa gtcggtgcct tcggtgctta
      301 tcgtgatgga ctggaagtct atggccgggc gatggtttcg caggatcgtc agttagtttc
      361 attacccgat ttctcacatt atgaactgta tgacaaaccg gaagcagttc aggaggcgct
      421 ggaaaaagtt attccttttt tcaacaccca tctgggataa gtttcatgag aaatattatg
      481 aaaaatgtac tgattcttgg tgctggcggt cagatagccc gccatgtgat taatcaactg
      541 gcagataaac aaaccattaa acaaacttta tttgcccgtc agccagcaaa aatccacaaa
      601 ccctatccaa ctaatagcaa gattattatg ggagatgtac tgaatcatgc agcactgaag
      661 caggccatgc agggacagga cgttgtgtat gccaacctta cgggggaaga tttagatatt
      721 caggcaaatt cagtgatcgc tgcgatgaaa gcctgtgatg ttaaacgtct gatctttgtt
      781 ctttccctcg gtatttatga tgaagttcca ggaaaatttg gcgaatggaa taatgcagtt
      841 attggtgagc ccctaaaacc attccgcagg gctgcggatg caattgaggc ttctggactg
      901 gaatatacaa ttcttcgccc tgcctggctg actgatgaag atattattga ttatgagctt
      961 acctcacgca atgaaccatt caaaggtacg atcgtatcac gtaagagtgt cgcagcatta
     1021 atcacagata tcatcgataa acctgagaaa cacattggtg agaatatagg tatcaatcaa
     1081 cccgtgactg atggtgacaa gccattattc atgtaaacca attaaatagt taccatcgta
     1141 atgtactatg tgcagtacga tttattaaac ttattaaggt gaactgatca atccgatgcg
     1201 attaacatgt gtagaaccag taagaataaa atacgccatg tatataacat tacgacttac
     1261 atctaagtca gcataattct aatatctgca catcaataaa aatgcaaaca acttccggca
     1321 aaaaaagtga tacaagatcg atgacatagc gttgaagaaa aggatttgat aatccgcaaa
     1381 atttgcacaa cac
//
LOCUS       DADMKF010000342         1376 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.347, whole genome shotgun sequence.
ACCESSION   DADMKF010000342 DADMKF010000000
VERSION     DADMKF010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1376
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.347"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            3..983
                     /locus_tag="JNC24_004305"
     CDS             3..983
                     /locus_tag="JNC24_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001346030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HAY9160870.1"
                     /translation="MVGVPVPDNIHPMIKDEAGVWSWRTPVLKGNLYEYFFNVDGVRS
                     IDIGTAMTKPQRQVNSSMVLVPGSYLDTRSVAHGDLIAITYHSNALQSERQMYVWTPP
                     GYTGMGEPLPVLYFYHGFGDTGRSAIDQGRIPQIMDNLLAEGKIKPMLVVIPDTETDA
                     KGIIPEDFVPQERRKVFYPLNAKAADRELMNDIIPLISKRFNVRKDADGRALAGLSQG
                     GYQALVSGMNHLESFGWLATFSGVTTTTVPDEGVAARLNDPAAINQQLRNFTVVVGDK
                     DVVTGKDIAGLKTELEQKKIKFDYQEYPGLNHEMDVWRPAYAAFVQKLFK"
     gene            998..>1376
                     /locus_tag="JNC24_004306"
     CDS             998..>1376
                     /locus_tag="JNC24_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HAY9160871.1"
                     /translation="MKLIITEDYQEMSRVAAHHLLGYMSKTRRVNLAITAGSTPKGMY
                     EYLTTLVKGKPWYDNCYFYNFDEIPFRGKEGEGVTITNLRNLFFTPAGIKEENIQKLT
                     IDNYREHDQKLAREGGLDLVVLGL"
BASE COUNT          359 a          315 c          363 g          339 t
ORIGIN      
        1 tagtggtggg tgttccggtt ccggacaata ttcacccgat gatcaaagac gaagcaggag
       61 tctggtcgtg gcgcacgcct gtcctgaaag gcaacctgta tgagtatttt ttcaatgttg
      121 atggtgtacg cagcattgat ataggcaccg caatgactaa gcctcagcgc caggttaact
      181 ccagtatggt tctggtgcca ggcagttatc tggatacgcg ttctgttgcg catggtgatt
      241 tgatcgccat aacttaccac tccaacgcat tgcaatctga acgtcagatg tatgtctgga
      301 ccccgccagg atacaccggc atgggcgagc ctttgccagt gctctatttc tatcacggtt
      361 ttggtgatac cggacgttcc gctatcgatc aggggcgtat cccgcaaatc atggataacc
      421 tgctggctga agggaaaatt aaaccgatgc tggtggtgat cccggatacc gaaaccgatg
      481 cgaagggcat tattcccgaa gatttcgtgc ctcaggaaag acgtaaagtc ttttatccgc
      541 tgaatgctaa agcggcagat cgcgaactga tgaacgatat tatcccgctg attagcaagc
      601 gttttaatgt ccgtaaagat gccgatggtc gcgcgctggc agggctttca caaggcgggt
      661 accaggcgct ggtttccgga atgaatcatc tggaaagctt tggctggctg gccacattca
      721 gtggtgttac cacgacaacc gtaccggatg aaggtgtcgc ggcccggctg aacgatccgg
      781 cagctattaa ccagcaacta cgtaatttta ctgtggtcgt tggtgataaa gatgtcgtaa
      841 ccggcaagga tatcgccggg ctgaaaactg agcttgagca gaaaaaaatt aagtttgatt
      901 accaggaata tcccggcctg aaccatgaaa tggatgtctg gcggcctgcc tatgcagcct
      961 tcgtacagaa attatttaaa taaaataagg cattaagatg aaattaatca ttaccgaaga
     1021 ttaccaggaa atgagccgtg tcgcggcaca ccatctgctt ggttatatgt cgaagacgcg
     1081 tcgtgttaac ctggcaatta ccgccggtag cacgcccaaa ggcatgtatg aatacctcac
     1141 caccctggtt aaaggtaagc cctggtacga taactgctat ttctataatt tcgatgaaat
     1201 tccctttcgc ggcaaagagg gagaaggcgt aacgattacc aatctgcgta atctgttttt
     1261 cacccctgcg gggatcaaag aagagaatat ccagaagctc actattgata actaccgcga
     1321 gcatgatcag aaactggcgc gtgaaggcgg cctggatttg gtggtgctgg gattag
//
LOCUS       DADMKF010000343         1341 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.348, whole genome shotgun sequence.
ACCESSION   DADMKF010000343 DADMKF010000000
VERSION     DADMKF010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1341
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.348"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            <1..184
                     /locus_tag="JNC24_004307"
     CDS             <1..184
                     /locus_tag="JNC24_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9160872.1"
                     /translation="TISIIIALFTIVPAICYLLSAIIAKRYYSLTTHNLKTVMEQLAQ
                     GKRRCQQQFTSQEVQN"
     gene            194..>1341
                     /gene="yicI"
                     /locus_tag="JNC24_004308"
     CDS             194..>1341
                     /gene="yicI"
                     /locus_tag="JNC24_004308"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HAY9160873.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQDNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPAWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPG"
BASE COUNT          335 a          318 c          351 g          337 t
ORIGIN      
        1 cacgattagc atcattattg cgctattcac gattgttccg gcgatctgtt atttgctgag
       61 cgcgattatc gctaaacgct actactcact cacgacgcac aatctgaaaa ccgttatgga
      121 acagctggct cagggtaaac gccgttgcca gcaacaattc acctctcaag aagtgcagaa
      181 ctaaggaacg gcaatgaaaa ttagcgatgg aaactggttg attcaacctg gcctcaattt
      241 gattcacccg cttcaggtgt tcgaggttga acagcaggat aatgaaatgg tggtctatgc
      301 tgccccccgt gatgtgcgtg aacgtacctg gcagcttgat acgcctttat ttacgctgcg
      361 ctttttctcc ccacaggaag gtattgtcgg tgtgcggatt gagcattttc agggggcgct
      421 gaataacggt cctcattatc cgctcaatat tttgcaggac gtgaaggtca caatcgaaaa
      481 cacagaacgt tatgctgagt ttaaaagtgg caacttaagc gcgcgtgtca gcaaaggtga
      541 gttctggtca ctggattttc tgcgcaacgg cgaacgtatt accggtagtc aggtgaaaaa
      601 taatggctac gtgcaggaca cgaataatca acgcaattat atgtttgagc ggcttgatct
      661 tggcgttggc gaaacagttt acggtctggg agagcgcttt actgccctgg tgcgcaatgg
      721 ccagacggta gagacctgga accgggacgg cggcacaagt actgaacagg cgtataaaaa
      781 tatcccgttc tacatgacta accgtggtta tggggtactg gtcaatcatc cccagtgtgt
      841 ctcttttgaa gtgggatcgg agaaagtctc caaagtgcag ttcagcgttg agagtgaata
      901 tctcgaatac tttgttatcg acggcccgac gccgaaagcg gtacttgatc gttatacccg
      961 ctttactggt cgtccggcgc tgccgcccgc gtggtccttc ggcctgtggc taaccacttc
     1021 atttaccacc aactacgacg aagcgacggt aaacagcttt atcgatggta tggcggaacg
     1081 caatctgccg ctgcatgttt tccactttga ctgtttctgg atgaaagcct tccagtggtg
     1141 cgattttgag tgggacccgc tgactttccc tgacccggaa gggatgatcc gccgcctgaa
     1201 agcgaaaggg ctgaaaatct gcgtctggat taacccctat atcggtcaaa aatcccccgt
     1261 ctttaaagag ttacaagaga aaggctattt actcaaacgc ccggacggtt cgctatggca
     1321 gtgggataaa tggcagccag g
//
LOCUS       DADMKF010000344         1294 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.349, whole genome shotgun sequence.
ACCESSION   DADMKF010000344 DADMKF010000000
VERSION     DADMKF010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1294
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.349"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            206..1112
                     /locus_tag="JNC24_004309"
                     /pseudo
     CDS             206..1112
                     /locus_tag="JNC24_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001384481.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
BASE COUNT          355 a          262 c          399 g          278 t
ORIGIN      
        1 gacctggtac gggtgggacg gtgacaggct gaccactgta cagacccaac agacaagaat
       61 ccagacggta taccagccgg gaagcttcac gccgctcctg agaatcgaaa cagagaatgg
      121 cgaacaggcg aaggcgcggc accggagcct ggcggaggtg ttgcaggagg acacgggggt
      181 gacgcttccg gcggagctgg cggtgatgct ggggaggctg gagcgggaac tgcgtgcggg
      241 tgcggtgagt gcggagagtg aggcatggct tgcgcagtgc gggctgacgg tggagcagat
      301 ggaaagccag atggaggcgg aatacatccc ggagcggagg ctgcatcttt accactgtga
      361 ccaccgggga ctgccgcagg cgctcatcag cccggaaggg gaaacggcgt ggcgcgggga
      421 gtatgacgag tgggggaacc agcttaatga ggagaacccg catcacctgt accagccgta
      481 ccgtttgccg gggcagcagt atgatgagga gtcgggactg tattacaacc gtcaccggta
      541 ctacgatccg ttgcaggggc ggtatatcac ccaggatccg atagggttga aggggggaat
      601 aaatctatac acgtatccac tggcaccaat acgttacacc actggggtta gaacgagtta
      661 taagtgtata tgggcctccc gctccagacc gggcaggtgc tgaaactcct cttgttctta
      721 ctgatatgac tggaggagtg actatttatt atgatcctga aacaggtgat tcaatgactt
      781 ttgatagtag caatagaatt gatcgtcgtt ctcaacgtgg tgctggtgat ccatatacag
      841 gtgaagtcgt tggttgtgag acaaatggat caggaatatc ggcagcatat ggcacaacga
      901 agatctatac tacagatact agagcaagat ggctacatgg tgggggatca tcgttgcgcg
      961 atccttatgc tcctcgacaa ggctggaaac caactatggg atgtacccgt gcacagaatg
     1021 aagatgtaga tgagttgtgt aagaaagtga caagttggat gtattcgcat cctggagaga
     1081 gaattagata tgaacgtttt aagacacgtt aatgcgatta acatgggagt gtgcattcta
     1141 attttggtta ttggatgtgc aaatgctaaa gttcttaaac gatgtatatt acccgctcct
     1201 gatggtggcg tagaaaaact atcaaaaccc aacttacatg gagtaattaa aaaagttgat
     1261 ttgaacacaa atgaagcgga aattaaaata aaag
//
LOCUS       DADMKF010000345         1293 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.350, whole genome shotgun sequence.
ACCESSION   DADMKF010000345 DADMKF010000000
VERSION     DADMKF010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1293
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.350"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            262..780
                     /locus_tag="JNC24_004310"
     CDS             262..780
                     /locus_tag="JNC24_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HAY9160874.1"
                     /translation="MPTPCYISITGQTQGNITAGAFTADSVGNIYVQGHEDEMLVQEF
                     LHNVTVPTTPQSGQPAGQRAHKPFIFTVALNKAVPLMYNALASGEMLPTTELHWWRTS
                     VEGKQEHYFTTRLTDSTIVDMKLHMPHCQDPAKREFTQLLEVSLAYRKIEWEHVKSGT
                     SGADDWRAPLEA"
     gene            990..>1293
                     /locus_tag="JNC24_004311"
     CDS             990..>1293
                     /locus_tag="JNC24_004311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HAY9160875.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLELSLVSQQ
                     FLSLEFPQVLDKMAYLTIWQGDDVQRRVKGVVTWFELGENDKNQMLYSMKVCPPLWR"
BASE COUNT          317 a          316 c          332 g          328 t
ORIGIN      
        1 ctttggccat tttatggagg caatacggac tttgaacagt tgggtatggt tatccagata
       61 tatattatta agttattgct gaataagaga tttaatgatt ttaaaaatag tttttacgac
      121 aaccgtaaat tctagctcgg tttgtatgta aaaaatactg aaaaatgatg cggttccgtg
      181 aagtgagctg catctgcctg tctgggatta acggcaggtc attgtaagta gtaattaacc
      241 acgttaacaa ggagtttaca aatgccaaca ccgtgttata tctccatcac tggtcagacc
      301 cagggaaata ttactgcagg tgcatttacc gctgattctg tcggcaacat ctatgtgcag
      361 gggcacgaag acgagatgct ggtgcaggaa ttcctgcata acgttactgt cccgaccact
      421 cctcagtccg gtcagcctgc gggtcagcgt gctcacaagc ctttcatttt taccgtggcg
      481 ctgaacaaag cggttccgct gatgtacaac gcactggcct ccggcgaaat gctgccaacc
      541 actgaacttc actggtggcg tacctctgtg gagggcaaac aggagcatta tttcactacc
      601 cgcctgactg attcgacgat tgtcgatatg aaactgcata tgccgcattg ccaggacccg
      661 gcgaaacgcg agttcaccca gttgctcgag gtttcactgg cctaccgcaa aattgaatgg
      721 gaacacgtca aatctggcac ctccggtgcc gacgactggc gcgcaccact ggaagcataa
      781 gttaagccaa cagcatccgg ctatcaggcc ggatgctgtt tttgtattac gccgtagaaa
      841 gaaaggttgt cagtaagggg ttacgttcac gggagatggc ggatgccgga aacttcggcg
      901 gtagcgtgcc tgaacgcaag cgtaacactg tactgtcaca gccgctttaa cagcgaactg
      961 tctgtttttc cttaaccccg gaggttgcta tgtcaaccgg attacgtttc acgctggaag
     1021 tggacggcct gccaccggat gcttttgcgg tggtctcctt tcatctgaac cagtcactct
     1081 cttcgctctt ttccctcgag ctctctctgg tcagccagca gtttctctcc cttgaatttc
     1141 cgcaggtact ggacaaaatg gcctacctga cgatatggca gggcgatgac gtacagcgcc
     1201 gggtgaaagg tgtggtgacc tggtttgaac tgggggagaa cgacaaaaac cagatgctgt
     1261 acagcatgaa ggtatgccca ccgctgtggc gca
//
LOCUS       DADMKF010000346         1282 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.351, whole genome shotgun sequence.
ACCESSION   DADMKF010000346 DADMKF010000000
VERSION     DADMKF010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1282
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.351"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            132..1151
                     /locus_tag="JNC24_004312"
     CDS             132..1151
                     /locus_tag="JNC24_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000878026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISEc11 family transposase"
                     /protein_id="HAY9160876.1"
                     /translation="MKYTPVGVDIAKHVIQIHFINEHTGEVVDKQLRRQDFLTFFGNR
                     EPCLIGMEACGGSQHWARELTKLGHKVRLLQARFVKAFVMGNKNDVMDARAIWMAVQQ
                     PGKEIAVKTEEQQSVLVLHRTRMQLVKFRTAQINALHGTLLEFGETIHKGRAAMEREF
                     PEALERMKERLPPYLIMVLENQYNRLNELDSLIEDIEKQLTSVARQNETCKRLLDIPG
                     VGPLIATAAVATMGEASAFKSGREFAAYVGLVPKQTGSGGKVRLLGISKRGDTYLRTL
                     FIHGARAVALVAKEPGPWITELKKRRPASVAIVAMANKLARTVWAITAHDRKYDRNHV
                     SIRPY"
BASE COUNT          368 a          285 c          357 g          272 t
ORIGIN      
        1 cacacaatta aacacggagc agtatgatgg ttaaaagggt gaaactggca ggtgtggagc
       61 ctcgcagatt tccggcatca tagtgcccaa tacgggatgc ataaagctcc ctcagactgg
      121 agactccaca aatgaaatat acaccggttg gcgttgatat cgcaaaacat gtcattcaga
      181 ttcacttcat caatgagcac acaggtgaag tggttgataa acagttgcgt agacaggatt
      241 ttctgacgtt cttcggcaac cgtgagccat gcctgattgg tatggaggcc tgtggaggtt
      301 ctcagcactg ggcacgggaa ctgacaaaac ttggtcataa agtccggttg ttgcaggccc
      361 gcttcgttaa ggcattcgtc atgggcaata agaatgatgt gatggatgcc cgggctatct
      421 ggatggcggt tcagcagccg ggtaaagaaa tcgccgtaaa aacagaagaa cagcagtcgg
      481 tactggttct gcaccgtacc cgcatgcaac tggtgaagtt ccggaccgca caaattaatg
      541 ccctgcacgg gacgttactg gagtttggtg aaaccatcca caaaggccgg gcagcgatgg
      601 agcgggagtt ccccgaagca ctggaacgga tgaaagagag actgccaccg tatctcatta
      661 tggttctgga aaaccagtac aaccgactga atgagctgga ctcactgata gaggatattg
      721 aaaaacagct taccagcgtg gcgaggcaga atgaaacctg taagcggttg ctggatattc
      781 ctggcgttgg accacttatt gcgacggcag cggtggccac catgggggaa gcatcagcgt
      841 ttaaatcggg gcgagagttc gccgcatatg ttggtctggt tccaaaacaa actggctccg
      901 gagggaaagt acgtctgctg gggataagca aacgtggtga cacttatctc aggacattat
      961 ttatccacgg tgcaagagcg gtggcattag tagctaaaga gcctggcccg tggataaccg
     1021 aactgaaaaa acgtcgtcca gccagtgtgg caatcgtcgc catggcaaac aagctggcac
     1081 gaacagtatg ggcgataacc gcccatgacc gtaagtatga caggaaccac gtcagtatca
     1141 gaccatatta atcgctgata ccattaaaca atgaactctt aacaaaaggg tgaatgctga
     1201 aaggttgcta tggcggccag agtgatgaca aagacaggta agaccgtgac tcactaaacc
     1261 tgaacagtat tttgggcttg aa
//
LOCUS       DADMKF010000347         1279 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.352, whole genome shotgun sequence.
ACCESSION   DADMKF010000347 DADMKF010000000
VERSION     DADMKF010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1279
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.352"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(66..392)
                     /locus_tag="JNC24_004313"
                     /pseudo
     CDS             complement(66..392)
                     /locus_tag="JNC24_004313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518821.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(481..>1279)
                     /locus_tag="JNC24_004314"
     CDS             complement(481..>1279)
                     /locus_tag="JNC24_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2778 domain-containing protein"
                     /protein_id="HAY9160877.1"
                     /translation="QMQNQMDPVYTPARKIHLYHCDHRGLPLVLISTEGATEWCAEYD
                     EWGNLLNEENPHQLQQLIRLPGQQYDEESGLYYNRHRYYDPLQGRYITQDPIGLKGGW
                     NFYQYPLNPITDIDPLGLATCLYSITLSMLSCVSDTQNDDNSYDVLTIPVASGNNGNN
                     MQCKNNPGCTHLQNRGPIPQGVWSWNVNGPGATNRKPNGIRLVPSANTETYNRDGFLT
                     QSCLNAFGPSLGPRFCSEGCITGSSNDMQKLNELIFSEPDSTLTVTD"
BASE COUNT          343 a          277 c          241 g          418 t
ORIGIN      
        1 ctattaactc tcaattttat atttttatca tcactacaaa tattaactga atttatatca
       61 atgatttttt tattcttatc ttttatttta tttactgttt caatgcaatc agcggcaagt
      121 tggagtggat ttagattttt cagatatggt cttatgatct ttcccttact taataaatag
      181 catgtatagg attcggaaag tccagcatct attttgtacc tcattatatt agataaggat
      241 gtgagaaatt ccttgttatc attcgcagcc atatatgcca gtccgagttc agcctctgcg
      301 gccttacagg caaaatagct tagtgaacac gcttttcgtc cctctttagt actggctgat
      361 attagtgccc ataatattgc tttgtctgcc aaatctaact ctctctgaaa ttgtcttgca
      421 gtaactgcac tcgcagaaat agagaaaaaa atacaattgt taataacttt gccatatttt
      481 ttagtccgta actgttaatg tgctatctgg ctctgaaaat attaactcat ttaatttttg
      541 catgtcatta gatgaaccag ttatacaacc ctcggaacag aatcgaggtc ctaaagacgg
      601 accgaaagcg tttaaacaac tttgtgttaa aaatccatca cgattatatg tttctgtatt
      661 tgctgacggt actaaacgta taccattagg ttttctattg gttgctccag gaccattaac
      721 attccagctc catacccctt gaggtattgg acctctattt tgtaagtgtg tacaaccagg
      781 attattttta cattgcatat tattaccatt attaccagag gcgacaggaa tagtaagcac
      841 atcataagag ttatcatcat tttgcgtatc tgaaacgcat gaaagcatac ttaaagttat
      901 gctgtataaa caggtcgcta atcccaacgg gtcaatatca gttatcggat tcaacggata
      961 ctgataaaaa ttccatcccc ccttcagtcc aatcggatcc tgagtgatat atcgcccctg
     1021 cagcgggtca taatagcggt ggcggttgta atacaggccg gactcctcat catactgctg
     1081 ccccggcagg cggataagct gctgcagctg atgcgggttc tcttcattca gcaggttgcc
     1141 ccactcatcg tattctgcgc accattctgt tgccccttcc gtgctgataa gcaccagcgg
     1201 caggccgcga tggtcgcagt ggtacaggtg gatttttcgc gccggcgtgt acaccgggtc
     1261 catctggttt tgcatctgc
//
LOCUS       DADMKF010000348         1265 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.353, whole genome shotgun sequence.
ACCESSION   DADMKF010000348 DADMKF010000000
VERSION     DADMKF010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1265
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.353"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(24..>1265)
                     /locus_tag="JNC24_004315"
     CDS             complement(24..>1265)
                     /locus_tag="JNC24_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HAY9160878.1"
                     /translation="IKHIIIAERAHQESLVKQKRIAQVWNHKTQLARELKKPMGKQAP
                     GWLELSEDGSYYIVDEDKASLVNIIYDKRLSGMSMFAICKWLNEQGYLTINQRKVRIS
                     KTKKPDGNWSALSVKHILTSRSVLGYLPAKISTEDRKTVLREEIEGFYPQIVTDSKFY
                     AVQQLLEETGKGKTSSGEHWLYVNILKGLIRCKCGLVMTPTGIRKPVYQGTYRCNGNK
                     ESRCSYGTVSRKLLDTQLCSRLFSKLSQLHDEATDTAKLDELQRRLNTVDGELEKLTE
                     TLIQLPNITQIQEALRVKQEEKDELIVQLSREKARVKSVSSLDLSGLDMESVEGRTEA
                     QIIIKRLVKEIVVSGNEKLVDIYLHNGNMIRGFPLDGKDDHTLTLEEATDEMQSLDDM
                     LIFGEPVTRIYPAGDMEEVDA"
BASE COUNT          334 a          298 c          227 g          406 t
ORIGIN      
        1 tcagtgaacc ctaacaaaaa atactaagcg tccacctctt ccatatcacc agcaggataa
       61 atccttgtta ctggttcacc aaaaataagc atatcatcta atgactgcat ctcatcggtt
      121 gcttcttcaa gcgttaaggt gtggtcatct ttaccatcaa gaggaaaacc acgaatcata
      181 tttccattgt gaagatatat atccaccagc ttttcattac cagaaacaac aatctctttc
      241 accagacgct taataataat ctgggcttca gttctcccct ctacagactc catatcaaga
      301 ccgcttaaat ccaagcttga tacagatttt accctcgcct tttctcgtga taactgaact
      361 atcagttcat ctttttcttc ctgtttaacc cgtagtgctt cttgaatctg agtgatgtta
      421 ggaagctgta tcagcgtctc agtgagtttt tccagttcac catcaacggt gtttaatctt
      481 ctttgtagtt catctaattt agctgtatcg gtagcttcat cgtgtagctg tgacagtttg
      541 ctaaacagac gactacaaag ctgtgtatca agtaactttc tggacactgt accgtagcta
      601 caacgggact ccttattacc attgcaacgg taagtacctt gataaacggg tttacgtatc
      661 cctgtaggag tcattacaag cccacactta caacggataa gccccttcag tatgttcaca
      721 tatagccagt gttcaccgct ggatgttttc cccttccctg tttcctctaa cagttgctgt
      781 acagcgtaaa acttagagtc tgtgacaatc tgaggataga acccttcaat ttcttcacgt
      841 aacactgttt tacggtcttc tgtagaaatc ttagcgggaa ggtaacctaa tacacttcgt
      901 gaagtcagga tgtgtttaac gcttaatgct gaccagttgc catccggttt cttagtcttg
      961 gatattctga ccttacgctg attaatggtc aggtaacctt gttcgtttag ccatttacag
     1021 atagcaaaca tcgacatacc agaaagacgc ttgtcataga tgatattcac cagtgacgct
     1081 ttatcttcat caacgatgta gtatgaacca tcttctgata gctctaacca tccgggggct
     1141 tgcttaccca taggcttctt aagttctctt gcaagctgtg ttttatggtt ccagacctga
     1201 gctatacgct tttgcttaac aagagattct tggtgtgctc tctcagctat gatgatgtgc
     1261 ttaat
//
LOCUS       DADMKF010000349         1233 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.354, whole genome shotgun sequence.
ACCESSION   DADMKF010000349 DADMKF010000000
VERSION     DADMKF010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1233
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.354"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(1..857)
                     /locus_tag="JNC24_004316"
                     /pseudo
     CDS             complement(1..857)
                     /locus_tag="JNC24_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706805.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
BASE COUNT          308 a          299 c          308 g          318 t
ORIGIN      
        1 aaagtctcca tggtacagac ccgtgcgcca tcaggaacgg tttcggtgaa tctgatgttg
       61 ccgcgttcgt cgaagtgaac aaccagggtg cggccatcgc ctggaattat tttgtcagtg
      121 ggtggggtgt tattctggcg cagttcggcc tccatgcgat cgaactcagc aatgtaggct
      181 tctttaaatg cagcggcttt tttgccagtg aatcccatca ccaggaaaac gaagccgttt
      241 ttggtgattt ggtacatcgg gcgtttttcg cctttggcgt cagtgtaggt gacgtcctca
      301 aaattgaggg cgttaaattc aggtgagcat tcagtatttg cgatggcacg taacacattg
      361 tcgtgacgct tgccaaagaa ctctgcgatc gcaatagacg tagtgacagc gcgaccattt
      421 tcgatggtta cgtcagggtg agaaagggca gggatagtag ccatgatggc atcctcgagt
      481 gataagttaa ttaactcacc accgaggttt tccacgacca taagggtggt gagacgtaca
      541 ggggtggaaa taccggtcac tcgagaaccc ggccagtctt gcgactgccc tgcacatccc
      601 accataattt gaatggagct gtgcattacg cataaaaaaa ccgcttcagc gcggctatgc
      661 gctcaagtaa cgttcgggtt tccacgcccg gcacccgttt tataaggtgc aggtgcacta
      721 taattccacc cgttctggtt ttcaatagct acattcaaca ttttctcttt cctttcatca
      781 ccgaagtgaa ctttgttgat gcggtgcctg gtgcctccag gtgacgttaa ccagttaaca
      841 attaacgccg gatacaggga aacccataac cacccgatac gggggaacta atcgcttttt
      901 aactgtgccg cgtgcgctga gccgcattca ccgcatcaca aaattcactt taaaaagggc
      961 ggacatcagc cagcaatgaa actgatgccg ccaaaggtac caatcaacat ggagtgttgt
     1021 ggcgtggttg tcacttaagc gtatggtcaa cctgacaacc cggtgtcctc aacggggaag
     1081 gaataactcc gccatactta ccgccgcgtc atttcgcgtt gtgtgcctgc ttttaaccac
     1141 gtcaggcgag gtggtatcct tcttatcccg aataaccaag aaggaaatct atatgactaa
     1201 agaagaattt gtctcttata tttttgataa aac
//
LOCUS       DADMKF010000350         1213 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.355, whole genome shotgun sequence.
ACCESSION   DADMKF010000350 DADMKF010000000
VERSION     DADMKF010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1213
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.355"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            231..>1213
                     /gene="gspA"
                     /locus_tag="JNC24_004317"
     CDS             231..>1213
                     /gene="gspA"
                     /locus_tag="JNC24_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspA"
                     /protein_id="HAY9160879.1"
                     /translation="MSTRREVILSWLREKRQTWRLCYLLGEAGSGKTWLAQQLQKDKH
                     RRVITLSLVVSWQGKAAWIVTDDNAAEQGCRDSAWTRDEMAGQLLHALHRTDSRCPLI
                     IIENAHLNHRRILDDLQRAISLIPDGQFLLIGRPDRKVERDFKKQDIELVSTGRLTEH
                     ELKASILEGQNIDQPDLLLTSRVLKRIALLCRGDRRKLALAGETIRLLQQAEQTSVFT
                     AKQWRMIYRILGDNRPRKMQLAVVMSGTIIAITCGWLLLSSFTAPFPVPAWLVPVTPV
                     VKQDMTKDIAHVVMRDSEALSVLYGVWGYEVPADSAWCDQAVRAGLACKSG"
BASE COUNT          329 a          249 c          310 g          325 t
ORIGIN      
        1 agcatcttta ttatgattcg ccagatggaa ggggaaacgt agtgtgggca cgatgtatgt
       61 tctaataaaa tagattgggt gggttgtata ttgacgttag tataaatagt attaatgccg
      121 cttgcatgaa tcaagcaaaa tttatttata ttagtaatat gtatttaatt aataaataca
      181 tacattattt ttatgattaa ggataatcaa taattaaaac aggaagttct atgtctacga
      241 gaagagaagt tattctttcc tggttgcgtg agaaacgtca gacctggcgt ctgtgctatt
      301 tgttgggtga ggctggaagt ggaaaaacct ggctggcgca gcaactgcaa aaagataaac
      361 atcgccgtgt gattacttta agcctcgttg tttcctggca aggtaaggcc gcatggatcg
      421 ttaccgacga taacgcggct gaacagggct gccgtgacag cgcctggacg cgagatgaga
      481 tggcggggca attactgcat gcgcttcatc gaactgatag tcgctgccct cttattatta
      541 ttgaaaatgc tcacctgaat caccgccgca tacttgatga tctccaacgc gcgatatctc
      601 ttattcctga cggtcaattt ttgctgatcg gcagacccga tcgaaaagtc gaacgtgatt
      661 ttaaaaaaca ggacattgaa cttgtctcga caggacgtct gacggagcac gaacttaagg
      721 caagcatcct tgaaggtcag aatattgacc aacccgatct ccttttaacc tccagagttc
      781 tgaaacggat agctttatta tgtcggggcg atcgcagaaa gctggcgctt gccggcgaaa
      841 caattcgctt attacagcaa gccgagcaga ccagcgtttt cactgcaaaa caatggcgaa
      901 tgatttaccg catattgggt gataaccgcc cccggaaaat gcagcttgcc gtggtaatga
      961 gtggaaccat tatcgctatt acctgtggtt ggctactgct gtcctctttc acagctccgt
     1021 tccccgttcc tgcgtggctg gtacccgtaa cgccagtagt taaacaagat atgactaagg
     1081 atattgctca tgtggtgatg cgcgatagtg aggcgctaag cgtgttatac ggcgtatggg
     1141 ggtacgaggt cccagctgat tcggcctggt gcgatcaggc agtaagagcg gggctggcat
     1201 gtaaaagtgg gaa
//
LOCUS       DADMKF010000351         1211 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.356, whole genome shotgun sequence.
ACCESSION   DADMKF010000351 DADMKF010000000
VERSION     DADMKF010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1211
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.356"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(<1..680)
                     /locus_tag="JNC24_004318"
     CDS             complement(<1..680)
                     /locus_tag="JNC24_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HAY9160880.1"
                     /translation="MLPVNNPPLSTGNVSFYRTTSIDNVHNNYLSEWVEWTKNSISGE
                     NRETAFTRLQLCLENSETSLDLSCLGLRSLPRLPDNLDEINVSNNQLSMLPELPRALK
                     ELNASSNQLSALPELPVSLEYINVSDNHLFALPELPASLEYINVSDNHLSVLPRLPMS
                     LELLDAARNALEVIPDFPERDDHIIRIFWLNQNRITAIPESILGLSSDSVVNLRENQL
                     SPRIMQTL"
     gene            complement(996..>1211)
                     /locus_tag="JNC24_004319"
     CDS             complement(996..>1211)
                     /locus_tag="JNC24_004319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HAY9160881.1"
                     /translation="DGTLYGATILGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLT
                     HILSEHFENVKTKVQGTVVMFSASGKK"
BASE COUNT          355 a          211 c          252 g          393 t
ORIGIN      
        1 aacaaagttt gcattattct gggagatagt tgattttctc taagattgac aacgctatca
       61 gaactgaggc caagtatgct ttccggaatt gccgtgatcc ggttctgatt aagccagaat
      121 attcttataa tatgatcatc tctttctgga aaatctggta ttacttccaa agcatttctg
      181 gctgcatcaa gtaattccaa tgacattggt aacctcggaa gtacagacag gtgattgtca
      241 cttacattaa tatattctag tgacgcaggt aattcaggaa gtgcgaacaa atggttatca
      301 ctcacattta tatattccag cgacactggt aattcaggaa gtgcagataa ttgattactg
      361 cttgcattca gctctttcaa tgcccttggt agctcgggga gcattgatag ttggttattg
      421 cttacattaa tttcatcaag attgtcaggc aatcgtggta gagatctgag acctaaacaa
      481 gataagtcca acgatgtttc actgttctcc agacataatt ggagccgggt aaaagcagtt
      541 tccctgtttt ctccggaaat gctgttttta gtccattcaa cccattcgga gagataatta
      601 ttgtgaacat tgtcgattga tgtagttctg taaaaagaga cgtttccagt ggataggggg
      661 ggattattta caggaagcat aataacctcg cagaaggata ttctgataaa atgtgatact
      721 gggaaataat aagctgaaat aaaatgtttc aggattgaaa tttattgttt tcattttaag
      781 gaagagatgt tgatttaact gtaaaaaaac gatactattt tcttagatgg taaatgtctc
      841 gcctgtgcta tattttgttt ttcggttagt taaatatcgt acgttcatat ttgaacgttc
      901 tgccggaatg cattatcaat agaggtaaag tcgcaacccc aaatcgtaaa ggaaaccgta
      961 gcacgtctta agcaagaacg tgctgcggtt ggatgctatt ttttccctga agcggaaaac
     1021 attactacag taccttgaac cttggtttta acattctcga aatgctctga gagtatatgt
     1081 gttaagcctt cttcggaatc ttttgtgttt gaaaagatgc ctttctgatt gtaaatgcgc
     1141 atcagttttt gaccgaagct attgtgcaca actccatcgc caagaattgt ggctccgtat
     1201 agagttccat c
//
LOCUS       DADMKF010000352         1198 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.357, whole genome shotgun sequence.
ACCESSION   DADMKF010000352 DADMKF010000000
VERSION     DADMKF010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1198
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.357"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            223..1038
                     /locus_tag="JNC24_004320"
     CDS             223..1038
                     /locus_tag="JNC24_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9160882.1"
                     /translation="MISLKAPHNNLMPYTQQSILNTVKNNQLPEDIKSSLVSCVDIFK
                     VLIKQYYDYPYDCRDDLVDDDKLIHLMAAVRDCEWSDDNALTINVQFNDFPGFYDWMD
                     YPDHPVKFVFRILENQKGTVWVYDQDDAFLDIKANVQAGRFTGLKKLVQFINSVRTDC
                     KCILLEHHMPLLRLFPKGKECMHVEKWLREMSSIPETDAPIKQALAHGLLLHLKNIYP
                     VFPESLVMLLLSVLDVKTYRDDARLNEWISNRVQELGDRYYPVNKHVKIRYTL"
BASE COUNT          364 a          230 c          233 g          371 t
ORIGIN      
        1 tggtttaaca tattaacttc gctctccact taacttttta gttaaggaga gcgaaataat
       61 atcaaagtag cagtaaaacc tataacgtaa attcaaattg ttaaattaac tccctccagt
      121 acacaatact tctcacgtta gttatgagcg atttctgata gtgcctggtt taatcagcgc
      181 tttattatct gcgacgttta tttttattta agagagatcg cgatgatatc attaaaagct
      241 ccgcacaata atttgatgcc atatacgcaa caaagcatac ttaatacggt taaaaacaat
      301 cagttaccag aggatatcaa aagctcccta gtttcctgtg tcgatatatt caaggttttg
      361 attaaacaat attatgatta tccttatgat tgtcgtgatg atttagtcga tgacgacaaa
      421 ctcattcatc tcatggctgc cgtacgagac tgtgagtggt cagacgataa cgcactcacc
      481 ataaatgtgc agtttaatga tttcccagga ttttatgact ggatggatta ccctgatcac
      541 ccggttaagt ttgtttttcg catacttgaa aatcaaaaag ggacagtttg ggtatatgac
      601 caggatgatg cgtttcttga tattaaagca aatgtgcagg ctggacgctt taccgggctt
      661 aagaaattag tgcagtttat caattcagtg cgaacagatt gcaaatgtat tcttcttgaa
      721 caccatatgc ctttgctgag acttttccct aaagggaaag agtgtatgca tgtggaaaaa
      781 tggcttcgcg aaatgtcgtc tattccggag acagacgcgc ccattaagca ggcgcttgcg
      841 catggattat tattacactt aaaaaatatc taccctgttt ttcctgaatc actggtgatg
      901 ttattgctct ctgttctgga cgtaaaaaca tacagagatg atgcacgatt aaatgaatgg
      961 atatctaata gagtgcagga attaggtgac agatattatc ctgtaaataa gcacgttaaa
     1021 atccgctaca ctctttagtt cagaaagggt tccacctgca agttgttatt ccaccagaga
     1081 gtgaatcgca aagcaggtaa tcatttcatc tgtatatccg tatgccaaat ggcattccac
     1141 cgccttttcg ccgatatttt gttgtgatcg gcgacacttc gcttaaaaaa gcaccagc
//
LOCUS       DADMKF010000353         1181 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.358, whole genome shotgun sequence.
ACCESSION   DADMKF010000353 DADMKF010000000
VERSION     DADMKF010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1181
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.358"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            <1..1000
                     /gene="htrE"
                     /locus_tag="JNC24_004321"
     CDS             <1..1000
                     /gene="htrE"
                     /locus_tag="JNC24_004321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Yad fimbria usher protein HtrE"
                     /protein_id="HAY9160883.1"
                     /translation="WASGQNRSNYSIGYSNSASWGSYSVSAQRSWNEDGDTDDSVYLS
                     FTIPIEKLLGTEQRTSGFQSIDTQMSSDFKGNNQLNVSSSGYSDNARVSYSVNTGYTM
                     NKASKDLSYVGGYASYESPWGTLAGSVSANSDNSRQVSLSTDGGFVLHSGGLTFSNDS
                     FSDSDTLAVVQAPGAQGARINYGNSTIDRWGYGVTSALSPYHENRIALDINDLENDVE
                     LKSTSAVAVPRQGSVVFADFETVQGQSAIMNITRSDGKNIPFAADIYDEQGNVIGNVG
                     QGGQAFVRGIEQQGNISIKWLEESKPVSCLAHYQQSSEAEKIAQSIILNGIRCQIQ"
BASE COUNT          358 a          236 c          276 g          311 t
ORIGIN      
        1 ctgggcttcc ggacaaaatc gtagcaatta ctctattggc tacagtaaca gtgcatcctg
       61 gggcagctac agtgtcagtg cccagcgttc atggaatgaa gacggcgaca ctgacgatag
      121 cgtttatctc agtttcacca ttcctattga aaaattactt ggcactgaac aacggacttc
      181 aggtttccag agtattgata ctcaaatgag cagtgacttt aagggtaata accaactcaa
      241 cgtcagtagc agtggctata gtgataacgc tcgcgtcagt tatagcgtaa atactggcta
      301 tacgatgaat aaagccagca aagatttgag ttatgttggg ggttatgcca gctatgaatc
      361 accgtgggga accctggcag gttcagtttc tgcaaatagc gataacagtc gtcaagtgtc
      421 tctcagcacc gacggtggtt ttgtattgca tagcggcggc ctgaccttca gtaatgacag
      481 ttttagcgac tccgatacac tggcggtagt tcaggctcca ggtgctcaag gagcgcgaat
      541 aaattatggc aacagtacta tcgatcgatg gggttatggt gtcaccagcg ctctttctcc
      601 ttatcatgaa aaccgtatcg cgctggatat caacgatctt gagaacgatg ttgaattaaa
      661 aagtaccagt gcagtagctg taccgcgtca gggttcagtc gtctttgctg attttgaaac
      721 cgtgcaaggg caatcagcca ttatgaacat cacacgaagt gatggtaaaa atattccatt
      781 tgctgcagat atttatgatg agcaaggcaa tgtcattggt aatgttggac agggtggaca
      841 agcatttgtt cgtggcattg agcagcaggg aaatatcagc attaaatggc tcgaagaaag
      901 taaacccgta agttgtcttg cgcattatca acaaagttca gaagcagaaa aaatagcgca
      961 atctattatt ctgaatggaa ttcggtgtca gattcagtaa ctacaaggaa cgtcaaatga
     1021 taaaaataac gccacataaa atagcgatac tgatgggaat attattatca ccatcagtat
     1081 ttgcaacgga tattaatgta gagtttacag ccactgtcaa agtagactgg ccccctgaat
     1141 ctccagacaa ccagtatcac ttaaataagt gatagtctta a
//
LOCUS       DADMKF010000354         1150 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.359, whole genome shotgun sequence.
ACCESSION   DADMKF010000354 DADMKF010000000
VERSION     DADMKF010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1150
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.359"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            <1..189
                     /locus_tag="JNC24_004322"
     CDS             <1..189
                     /locus_tag="JNC24_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAY9160884.1"
                     /translation="VREYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPTTSTN
                     NYRIALRKYQHYQRQTCT"
     gene            322..1122
                     /gene="tcyJ"
                     /locus_tag="JNC24_004323"
     CDS             322..1122
                     /gene="tcyJ"
                     /locus_tag="JNC24_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY9160885.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKERGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTADDLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
BASE COUNT          318 a          248 c          307 g          277 t
ORIGIN      
        1 gtgcgtgaat atgtcgttcg tctgcgccgt ttgggtaatc atctacacga gcaaaatatt
       61 tccctcgatc agctgcagga cggtttcctt gatgaaatcc tcgccccgtg gctgcccacc
      121 accagcacca acaattaccg cattgcgtta cggaagtatc aacactatca gcgtcaaacg
      181 tgtacctgac ttgtgcagaa atcctccagt ctgcctgctt ctgatatata ttaaataaga
      241 ataagatgta gcggagttgt ttttgtgttt acaaacaatg gctctacact gcaaacagac
      301 ataacaacat tcggggtgaa tatgaaatta gcacatctgg gacgtcaggc attgatgggt
      361 gtgatggccg tggcgctggt tgcgggtatg agcgttaaaa gttttgcaga tgaaggtctg
      421 cttaataaag tcaaagagcg cggcacgctg ctggtagggc tggaaggaac ttatccgccg
      481 ttcagttttc agggggatga cggcaaatta accggttttg aagtggaatt tgcccaacag
      541 ctggcaaaac atcttggcgt tgaggcgtca ctaaaaccga ccaaatggga cggtatgctg
      601 gcgtcgctgg actctaaacg cattgatgtg gtgattaacc aggtcaccat ttctgacgag
      661 cgcaagaaaa aatacgattt ttctacccct tacaccattt ccggtattca ggcgctggtg
      721 aaaaaaggta acgaaggcac cattaaaaca gccgatgatc tgaaaggcaa aaaagtgggt
      781 gtcggtctgg gcaccaacta tgaagagtgg ctgcggcaga atgttcaggg tgtcgatgtg
      841 cgtacctatg atgatgaccc gaccaaatat caggatctgc gcgtaggccg tatcgatgcg
      901 atcctcgttg accgtctggc ggcgctggat ctggtgaaga aaaccaacga tacgctggca
      961 gtaaccggtg aagcattttc ccgtcaggag tctggcgtgg cgctgcgtaa aggaaatgaa
     1021 gatctgctga aagcagtgaa tgatgcaatt gcggaaatgc aaaaagatgg cactctgcaa
     1081 gccctttccg aaaaatggtt tggtgctgat gtgaccaaat aatcagcata atgacaaaaa
     1141 agggcgcttt
//
LOCUS       DADMKF010000355         1149 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.360, whole genome shotgun sequence.
ACCESSION   DADMKF010000355 DADMKF010000000
VERSION     DADMKF010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1149
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.360"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            248..493
                     /locus_tag="JNC24_004324"
     CDS             248..493
                     /locus_tag="JNC24_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9160886.1"
                     /translation="MAVQLFKTLLNQIPLLSSLQSGTLPLFGYSGRVRPMKKAHTGES
                     GLKWEAKDSSKLIGNKDHALRGLSSPIVGIRQIRLIT"
     gene            complement(510..1130)
                     /locus_tag="JNC24_004325"
     CDS             complement(510..1130)
                     /locus_tag="JNC24_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308409.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HAY9160887.1"
                     /translation="MDPLHAVYLTVGLFVITFFNPGANLFVVVQTSLASGRRAGVLTG
                     LGVALGDAFYSGLGLFGLATLITQCEEIFSLIRIVGGAYLLWFAWCSMRRQSTPQMST
                     LQQPISASWYVFFRRGLITDLSNPQTVLFFISIFSVTLNAETPTWARLMAWAGIVLAS
                     VIWRVFLSQAFSLPAVRRAYGRMQRVASRVIGVFALRLIYEGVTQR"
BASE COUNT          346 a          274 c          274 g          255 t
ORIGIN      
        1 gtttcgcagc catgcggtgt ataaaaaatg atctcatgca gatgttttgt gaatgtgtgg
       61 gttgacattc atatgaaaaa aatcataatt ccatcatgtt tgtgtggggt cttttctgta
      121 ccttacgcat cgcactcaag cctgacagaa aatatgctgt aaggctcata tcaaaaaccg
      181 ccattagctc atcaggaaga gcagacgaca accaggattg ttgtatggta cggggttcga
      241 ggcctcgatg gcggtccaac ttttcaaaac tcttctgaat cagatcccct tattatcatc
      301 cctgcaaagt ggaacattgc cattgtttgg ctattcagga cgggtcagac caatgaaaaa
      361 agcccacaca ggggagagtg ggctgaaatg ggaagctaaa gactcaagta aacttatcgg
      421 aaataaggac cacgcattac gggggctatc atcgccgata gtcgggattc ggcaaatcag
      481 gttgattacg tagagagtaa attatctgct caccgctgcg tcaccccttc gtaaatcagg
      541 cgtagcgcga atacaccaat aacccgactg gcaacgcgtt gcatacgccc ataagcacga
      601 cgcacagcgg gcaaagaaaa cgcctgacta agaaagactc gccagataac tgatgcgagc
      661 acaatccccg cccaggccat taaacgtgcc catgttggtg tttcggcatt taatgttact
      721 gagaaaatac tgataaaaaa taaaacggtt tgcgggttag agagatcggt aattaatccg
      781 cggcgaaaaa agacatacca ggaggcgcta atcggttgtt gtagtgtgct catttgcggt
      841 gttgactggc ggcgcatgct gcaccacgca aaccataaga gataagcgcc gccgacgatt
      901 ctgataagcg aaaaaatctc ctcacactgc gttattagcg ttgcaagacc aaacaaaccc
      961 aacccggaat aaaatgcatc gcccagcgcc acgcccagtc cggtcagcac ccctgcgcgt
     1021 cgaccggaag ccaggctggt ttgtactacc acaaagagat tggctcccgg attaaaaaaa
     1081 gtaatcacga acagtcctac ggtcaggtaa acggcgtgca aaggatccat agtgatttca
     1141 tccataaat
//
LOCUS       DADMKF010000356         1139 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.361, whole genome shotgun sequence.
ACCESSION   DADMKF010000356 DADMKF010000000
VERSION     DADMKF010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1139
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.361"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(<1..485)
                     /locus_tag="JNC24_004326"
     CDS             complement(<1..485)
                     /locus_tag="JNC24_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9160888.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGMGIAQTKTLAKLANHAAKKWQRQT
                     GG"
     gene            complement(485..904)
                     /gene="umuD"
                     /locus_tag="JNC24_004327"
     CDS             complement(485..904)
                     /gene="umuD"
                     /locus_tag="JNC24_004327"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HAY9160889.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
BASE COUNT          319 a          299 c          239 g          282 t
ORIGIN      
        1 ccccccgtct gccgctgcca tttttttgcc gcatgattgg caagcttagc cagcgttttg
       61 gtctgggcga tccccatacc aacagtaaga tgggtacgtt gtagcaccgt tgcgcgaatt
      121 tctctgccaa aatcagtcag atcgcgacaa ttacgcacac ctgtcagatc gcagaatgcc
      181 tcatcaatac tgtaaatctc gacgcggggc gatagttctt ccagcgtcga catcacccga
      241 ttgctcatgt ctgcgtaaag ctcataattg ctgctaaagc aaaccacacc acagcgacga
      301 aacagatctt tttgtttgaa ccagggatcg cccattttaa cacccagcgc ctttgcctca
      361 gcgtttcggg cgataacgca accgtcatta ttcgatagca caaccaccgg tttaccccat
      421 aaatcagggc gaaacaccgt ctcacagctg gcataaaacg cgtttacatc acagagagca
      481 aacatcagcg catcgcctta acgacgtgga tcaccacacc aaagacatcc agcgtatctt
      541 cgctactgat ggtaatgggc gagtacgcac tgttcatggg aataagctgt accgtcgggc
      601 gtagttgcaa ttttttcacc gtaaactcgc cgtcaacagc agcgatgaca atatcaccat
      661 ggctggcggt aatagcgcta tcgacaatca gtaaatcacc gtcactaatt ccaccatcaa
      721 tcatagaatc accacttgct ttgacgaagt aagtcgcgct gggatgctgg atcaacagtt
      781 gattcagatc gatgcgctgt tcgacgtaat ctgctgccgg tgaaggaaag ccacactgaa
      841 caagatcgct aaatagcgga aaagtcacaa tttcgcggag atccgcaggc ttgataaaca
      901 acataataat ctgcctgaag ttatactgtt tttatataca gtaatctgtt cttgccagca
      961 gatcaatact gattcaggct atcaatattt gtcgctgcat agactgctga tttttcgttc
     1021 tcttatcctg tgctcacgtg gccttctggc gacgacgctc atccagcaga aatgaaaaat
     1081 atcacccggc aaaaaaatag aatagaagca ttcgccataa tgacattaaa cattgtttg
//
LOCUS       DADMKF010000357         1118 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.362, whole genome shotgun sequence.
ACCESSION   DADMKF010000357 DADMKF010000000
VERSION     DADMKF010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1118
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.362"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            44..238
                     /locus_tag="JNC24_004328"
     CDS             44..238
                     /locus_tag="JNC24_004328"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9160890.1"
                     /translation="MLPSISINNTSATYPESINENNNDEVNGLVQELKNLFNGKEGIS
                     TCIKHLLELIKNAIRVNDDP"
     gene            427..1112
                     /locus_tag="JNC24_004329"
                     /pseudo
     CDS             427..1112
                     /locus_tag="JNC24_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000577525.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          437 a          190 c          192 g          299 t
ORIGIN      
        1 gtaatatata tttgtaacaa atcaatcaaa atggaataaa atcatgctac catctatttc
       61 aatcaacaat accagcgcaa cttacccaga atccattaat gaaaataaca atgatgaagt
      121 taatggatta gtacaagagc tcaaaaacct ttttaatggt aaggaaggaa taagcacctg
      181 tattaaacat ctacttgagc ttataaaaaa cgccatacga gtaaacgacg atccttaaga
      241 tttaatatta ataattcctc agttacttat attgatattg gctccaatga tacagaccat
      301 attactattg gtatcgacaa ccaagaacca atagaattac ctgcgaacta taaagacaaa
      361 gaactcgtcc gtactatcat taatgacaac atagttgaga agactcatga tatcaataac
      421 aaggaaatga tcttcagcgc attaaaagaa atatatgatg gagatcctgg ttttatcttc
      481 gataagatat cacacaaact cagacatacg gtaacagaat ttgatgagag cgggaatagc
      541 gaaccaacgg acttatttac ctggtacggt aaagataaaa aaggcaactc tctcgctatt
      601 gtaattaaaa ataaaaacgg aaatgattac ttatctctcg gttactacga tcaggacgac
      661 taccacattc aaagaggaat tcgtattaat ggtgatagtc tcacccaata ttgtagtgaa
      721 aaagccagga atgcttcagc aatgcttcag cgtggtttga aagcagtaaa gctatcatgg
      781 cagaatcatt tgcaacaggt tccgatcatc aggttgtaaa cgagctcaac ggggaaagac
      841 tgagagaacc taacgaagtt tttaaacgtt taggtcgagc aataagatat aattttcaag
      901 tggacgatgc aaaatttaga cgcgacaatg taaaagaaat aatttctaat ttattcgcta
      961 acaaagttga cgttgaccat cctgaaaata aatacaaaga ctttaaggat ctcgaagata
     1021 acgttgaaaa aagacttcaa aatcgccagg cgaaatatca aaatgaaatt aatcaactat
     1081 ctgcgccagg tgttaatttt gatgatattt aaaattat
//
LOCUS       DADMKF010000358         1117 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.363, whole genome shotgun sequence.
ACCESSION   DADMKF010000358 DADMKF010000000
VERSION     DADMKF010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1117
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.363"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            84..1115
                     /locus_tag="JNC24_004330"
     CDS             84..1115
                     /locus_tag="JNC24_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630-like element IS630 family transposase"
                     /protein_id="HAY9160891.1"
                     /translation="MPIIAPISRDERRLMQKAIHKTHDKNYARRLTAMLMLHRGDRVS
                     DVARTLCCARSSVGRWINWFTQSGVEGLKSLPAGRARRWPFEHICTLLRELVKHSPGD
                     FGYQRSRWSTELLAIKINEITGCQLNAGTVRRWLPSAGIVWRRAAPTLRIRDPHKDEK
                     MAAIHKALDECSAEHPVFYEDEVDIHLNPKIGADWQLRGQQKRVVTPGQNEKYYLAGA
                     LHSGTGKVSCVGGNSKSSALFISLLKRLKATYRRAKTITLIVDNYIIHKSRETQSWLK
                     ENPKFRVIYQPVYSPWVNHVERLWQALHDTITRNHQCSSMWQLLKKVRHFMETVSPFP
                     GGKHGLAKV"
BASE COUNT          303 a          285 c          301 g          228 t
ORIGIN      
        1 tagatcactt tgagggaact tagcccggat tgtgcgatct gatcaatcgc caaatcaaaa
       61 caaatcacca accggactga gcaatgccga tcatagcacc aatttcccgt gacgaacgac
      121 gcctgatgca gaaagccatc cataaaacac acgataaaaa ttatgcccgc agactgactg
      181 ccatgctgat gctgcaccgg ggcgaccgtg tcagcgacgt tgccagaacg ctctgctgcg
      241 cccgttcctc tgttggacgc tggattaact ggttcacgca gtcgggtgtt gagggactga
      301 aatcattacc tgccgggcgt gcccgtcgct ggccgtttga gcatatctgc acactgttac
      361 gtgagctggt aaaacattct cccggcgact ttggctacca gcgttcacgc tggagtacag
      421 aactgctggc aataaaaatc aatgagataa ccggttgcca gttaaatgcc ggaaccgttc
      481 gccgctggtt gccgtctgcg gggattgtgt ggcgaagggc tgcgccaact ctgcgtatcc
      541 gtgacccgca taaagatgaa aagatggcag caatccataa agcactggac gaatgcagcg
      601 cagagcatcc ggtcttttat gaagatgaag tggatatcca tcttaatccc aaaatcggtg
      661 cggactggca actgcgcgga cagcaaaaac gggtggtcac gccgggacag aatgaaaaat
      721 attatctggc cggagcgctg cacagcggga caggtaaagt cagctgtgtg ggcggcaaca
      781 gcaaaagttc ggcgctgttc atcagcctgc tgaagcggct taaagcgaca taccgtcggg
      841 cgaaaaccat cacgctgatc gtggacaact acattatcca caaaagccgg gaaacacaga
      901 gctggctgaa ggagaacccg aagttcaggg tcatttatca gccggtttac tcgccatggg
      961 tgaatcatgt tgaacggcta tggcaggcac ttcacgacac aataacgcgt aatcatcagt
     1021 gcagctcaat gtggcaactg ttgaaaaaag ttcgccattt tatggaaacc gtcagcccat
     1081 tccccggagg caaacatggg ctggcaaaag tgtagcg
//
LOCUS       DADMKF010000359         1107 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.364, whole genome shotgun sequence.
ACCESSION   DADMKF010000359 DADMKF010000000
VERSION     DADMKF010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1107
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.364"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(<1..295)
                     /locus_tag="JNC24_004331"
     CDS             complement(<1..295)
                     /locus_tag="JNC24_004331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001572122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HAY9160892.1"
                     /translation="MSRKSCLIRLFKTTVSCKLFTAIILSVFLGQPALTYAGVVIGGT
                     RVVYLSNNADKSISVFSKEEKIPYLIQAWVDPFNKEDKSKAPFTVIPPVSRLEP"
     gene            complement(343..915)
                     /locus_tag="JNC24_004332"
     CDS             complement(343..915)
                     /locus_tag="JNC24_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA-O113"
                     /protein_id="HAY9160893.1"
                     /translation="MKRNIIGGAFTLASLMLAGHALAEDGVVHFVGEIVDTTCEVTSD
                     TADQIVPLGKVSKNAFSGVGSLASPQKFSIKLENCPATYTQAAVRFDGTEAPGGDGDL
                     KVGTPLTAGNPGDFTGTGQAIAATGVGIRIFNQSDNSQVKLYNDSAYTAIDAEGKAEM
                     KFIARYVATNATVTAGTANADSQFTVEYKK"
BASE COUNT          345 a          238 c          206 g          318 t
ORIGIN      
        1 aaggttctaa tcgtgatact ggcggaataa cggtaaacgg cgctttgctt ttatcctctt
       61 tattaaatgg atctacccaa gcctggatga gataaggtat tttttcttct ttgctaaata
      121 cagaaataga tttatcagca ttattactta agtaaaccac ccgcgtacca ccaatgacaa
      181 cgcctgcata agttaatgct ggttggccta aaaaaacgct gagtataatc gcggtaaata
      241 acttacatga aactgttgtt ttaaagagcc tgattaaaca actttttctg gacataacga
      301 ctccctggcc aaagccaact gaataaaaag gcccttgata aattatttct tatattcgac
      361 agtaaattgt gaatcagcgt tcgccgtacc agccgttacg gtcgcattgg ttgccacata
      421 gcgtgcaata aacttcattt cagccttgcc ttcagcatcg atagcggtat aagcagagtc
      481 gttataaagt ttaacctgcg aattatcgga ctggttaaaa atacgaatac caacgccggt
      541 tgccgcaatc gcttgtcctg taccggtaaa atcaccaggg ttgcctgctg taagcggcgt
      601 acccactttc aggtcgccgt cgccgccagg cgcttctgta ccatcaaaac gaacggctgc
      661 ttgagtgtac gttgccgggc aattttcaag tttaatactg aacttctgtg gcgacgccag
      721 actacctaca cctgaaaatg catttttact gactttaccc agtgggacaa tttgatcggc
      781 tgtatcggag gtaacttcac aagtagtgtc gacaatttca ccgacgaagt gaacaacacc
      841 atcttctgcc agtgcatgcc cggccagcat tagagatgcc agagtgaatg caccgcctat
      901 aatattacgc ttcataaaaa ctccattgat ataatatatc ctttaccatg aatcatcact
      961 tatattaata agtgtatttt tgattcaaag gaatagtaca ccaaagagac ggctttatct
     1021 aaaaataata ttaattacgc aaaaataaaa tataaattca ttgtttaaat attatttaaa
     1081 cagtaaaaaa tcaagtttgt tatcaga
//
LOCUS       DADMKF010000360         1107 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.365, whole genome shotgun sequence.
ACCESSION   DADMKF010000360 DADMKF010000000
VERSION     DADMKF010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1107
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.365"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(<1..93)
                     /locus_tag="JNC24_004333"
     CDS             complement(<1..93)
                     /locus_tag="JNC24_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9160894.1"
                     /translation="MSEENKENGFNHVKTFTKIIFIFSVLVFNDN"
     gene            232..903
                     /gene="queE"
                     /locus_tag="JNC24_004334"
     CDS             232..903
                     /gene="queE"
                     /locus_tag="JNC24_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HAY9160895.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVISRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYDVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
BASE COUNT          307 a          226 c          252 g          322 t
ORIGIN      
        1 gttatcatta aaaactaata cagaaaaaat aaatataatt ttggtgaatg ttttgacatg
       61 attaaatcca ttttctttat tttcttctga cataatacgt tactcccttt aattattaac
      121 gatatgtttc gcatttttct ttaaatctgc ctgtaacaga atgttaataa gacatgattg
      181 ttgtggcagg atctgcaata attaacaatt atccacttca cagagaatgc tatgcagtac
      241 ccgattaacg agatgttcca gaccctgcaa ggtgagggtt actttaccgg cgtccccgcc
      301 atttttattc gtttacaggg atgcccggtt ggctgtgcct ggtgcgacac caaacacacc
      361 tgggaaaagc ttgaggatcg ggaagtctcc cttttcagca ttctggcgaa gaccaaagag
      421 agtgataagt ggggggcggc gagcagtgaa gacttgttgg ctgtcattag tcgccaggga
      481 tacaccgcgc ggcatgtggt gattacgggt ggtgagcctt gcattcatga tttgctacca
      541 ctgactgatc tgctcgaaaa gaacggtttt agctgccaga tcgaaaccag tggtactcat
      601 gaggtacgct gcacaccgaa tacctgggtt accgtatcgc caaagctgaa catgcgcggc
      661 ggctatgacg tgttgtcaca ggcactggag cgagccaacg aaatcaagca tccagtgggg
      721 cgcgtacgcg atattgaagc actggatgaa ctattggcaa cgctgaccga tgataaaccg
      781 cgagtcattg cactgcagcc gattagccaa aaggatgatg ccacacgttt gtgcattgaa
      841 acctgcattg cgcgtaattg gcgtttgtcg atgcaaacac ataaatatct aaatattgcc
      901 tgattaaaca tttataagcg ttataaatgg gtggaacctg taaagacttc tactcattta
      961 tattgtttgt cgcctctgaa aactcctcca ttttacccat ccagggctaa tcattagcat
     1021 tctctacaaa ttctgtggca ttaatttttc gctggagtga aaattattgc ggtaaagttt
     1081 ggtagatttt aatttgtata gagttat
//
LOCUS       DADMKF010000361         1096 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.366, whole genome shotgun sequence.
ACCESSION   DADMKF010000361 DADMKF010000000
VERSION     DADMKF010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1096
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.366"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            <1..220
                     /locus_tag="JNC24_004335"
     CDS             <1..220
                     /locus_tag="JNC24_004335"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY9160896.1"
                     /translation="EGASRLSLPEGTLGQWVTAARKGLGTPGSRTVAELESEILQLRK
                     ALNEARLERDILKKATAYFAQESLKNTR"
     gene            256..1074
                     /locus_tag="JNC24_004336"
     CDS             256..1074
                     /locus_tag="JNC24_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAY9160897.1"
                     /translation="MCQVFGVSRSGYYNWVQHEPSDRKQSDERLKLEIKVAHIRTRET
                     YGTRRLQTELAENGIIVGRDRLARLRKELRLRCKQKRKFRATTNSNHNLPVAPNLLNQ
                     TFAPTAPNQVWVADLTYVATQEGWLYLAGIKDVYTCEIVGYAMGERMTKELTGKALFM
                     ALRSQRPPAGLIHHSDRGSQYCAYDYRVIQEQSGLKTSMSRKGNCYDNAPMESFWGTL
                     KNESLSHYRFNNRDEAISVIREYIEIFYNRQRRHSRLGNISPAAFREKYHQMAA"
BASE COUNT          312 a          261 c          286 g          237 t
ORIGIN      
        1 tgaaggcgct tcccgattat ctcttcctga aggcacttta ggacaatggg ttaccgccgc
       61 cagaaaaggg ctcggtactc ctggttcccg cacggtggct gaactggaat ctgaaattct
      121 gcaactgcgt aaggcgttaa atgaagctcg ccttgagcga gatatattaa aaaaagcaac
      181 agcgtatttt gcacaggagt cgctgaaaaa tacgcgttaa tcgaacaatg gcgacaacaa
      241 tttcccattg aagcgatgtg tcaggtattt ggtgtatcca ggagcggtta ttacaactgg
      301 gtacagcatg aaccctcaga cagaaaacaa agtgatgagc ggctaaaact ggagattaag
      361 gtggcacata tccgcactcg cgaaacatat ggaacccggc ggctccagac ggagctggca
      421 gagaatggca tcatcgttgg tcgtgaccga ctggcacgtc ttcgtaagga gctaaggcta
      481 cgctgtaagc agaaacgcaa gttcagagcg actacgaact cgaaccacaa tctgccagtt
      541 gcgccaaatc tgctgaacca gacgttcgct cctacagcac caaatcaggt ctgggtggcg
      601 gacctgacgt atgttgccac acaggaggga tggttgtacc tcgctggcat caaagatgtt
      661 tatacgtgcg aaattgtcgg ctacgccatg ggagagcgca tgacaaaaga gctgacaggt
      721 aaagccctgt ttatggcgct caggagccag cgcccacctg ccgggctaat ccaccactct
      781 gatcgaggtt cacagtactg cgcatacgat taccgggtca tacaggagca gtctggtctg
      841 aaaacatcaa tgtcgcgtaa aggtaactgt tacgacaacg ctccgatgga aagcttctgg
      901 ggaacgctga aaaatgagag cctgagccac tatcgtttta ataaccggga tgaagccatc
      961 tcagtaatac gggaatacat tgagattttc tacaatcgtc agcgtcgtca ctctcgtctg
     1021 gggaatatct ccccggcagc cttcagggaa aaatatcatc agatggctgc ttaaaaaaag
     1081 aacaaatggt agtgtc
//
LOCUS       DADMKF010000362         1087 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.367, whole genome shotgun sequence.
ACCESSION   DADMKF010000362 DADMKF010000000
VERSION     DADMKF010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1087
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.367"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(<1..645)
                     /locus_tag="JNC24_004337"
     CDS             complement(<1..645)
                     /locus_tag="JNC24_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAY9160898.1"
                     /translation="MSKISDLNYSQHITLADNFKQKSEVLNTWRVGMNNFARNAEGQD
                     NTRNILDPKTFLEFLVKIFTLGYVDFSKRSNEAGRNMMAHIESSSYIKNNDGSEIMKF
                     VMNNPEGERADLSKVEIEITLSAFTTMGTRQGHTAIIFQQPDGSTNRYEGKSFERKDE
                     SSLHLITNKILACYQREANKEIARLLNIPQELNNSQDLNNSQVSCKDSVDSTITD"
     gene            complement(768..>1087)
                     /locus_tag="JNC24_004338"
     CDS             complement(768..>1087)
                     /locus_tag="JNC24_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HAY9160899.1"
                     /translation="YLISEDMTEPHEARRTEYHARYLDIQIVLKGQEGMTFSTQPAGT
                     PDTDWLADKDIAFLPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKM
                     LMA"
BASE COUNT          313 a          247 c          191 g          336 t
ORIGIN      
        1 atccgtaatc gtagaatcaa ctgaatcttt acaactcacc tgcgagttat tcaagtcctg
       61 agaattattt aactcctgcg gaatatttaa tagacgagct atttccttgt tagcttcgcg
      121 ttggtaacat gccagaatct tgttagtaat caggtgtaat gaactctcat cttttctttc
      181 aaaggacttt ccttcataac gattagtcga accatcaggt tgttgaaata taatagccgt
      241 atgtccttga cgagtcccca tagtagtgaa agcggaaagt gtgatttcta tttccacctt
      301 agataaatcc gctcgttccc cttcaggatt attcataaca aacttcatta tctcactgcc
      361 atcattattt ttgatataag atgaggactc aatatgagcc atcatatttc ttcccgcttc
      421 gttggagcgt ttgctaaaat ccacataacc tagagtaaat atttttacca aaaattccaa
      481 aaatgtctta ggatcaagga tatttcttgt gttatcctgc ccctcggcat tacgggcaaa
      541 attattcatt ccaacacgcc aggtatttaa aacttcactt ttttgtttaa aattgtcggc
      601 taatgtaatg tgttgagaat aattcaaatc tgatattttg ctcatgacga actccttgag
      661 ttaataagaa cgtctaagca ttacaaattt tactcacaaa aaaatatcaa aaaacgctct
      721 tttcacaaac tcgccgtaaa cctggagtta tttacggcga aaagtcatca cgccatcagc
      781 atcttcacta ctgctttgcg aacctgggct ggtgcaccca ctgcgcacag cggtttatgc
      841 acttcccccg gataaaacac aacaaaatca ccttcattaa ggataactgt tttctcatca
      901 acgccttccg gcaaaaatgc gatgtcttta tcagctaacc agtcggtatc cggcgtgcct
      961 gcaggttgcg tgctgaaggt catgccttcc tgacctttta acacaatctg aatgtcgaga
     1021 tagcgggcat ggtactccgt acggcgagct tcgtgcggct cggtcatatc ttccgagata
     1081 agataaa
//
LOCUS       DADMKF010000363         1075 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.368, whole genome shotgun sequence.
ACCESSION   DADMKF010000363 DADMKF010000000
VERSION     DADMKF010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1075
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.368"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            6..1075
                     /locus_tag="JNC24_004339"
                     /pseudo
     CDS             6..1075
                     /locus_tag="JNC24_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000015058.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
BASE COUNT          264 a          309 c          335 g          167 t
ORIGIN      
        1 cgtagggtga agccgggctg aagcgggtgg tgaaaaagga acacgcggac ggcagcgtca
       61 cgcagagtca gtttgacgcc gtgggcaggc tcaggacaca gacggatgcc gcaggccgga
      121 caacagaata cagcccggat gtggtgacgg gcctcatcac gcgcatcacc acgccggatg
      181 gcagggcatc ggcgttttac tataaccacc acagccagtt aacgtcagcc accgggcctg
      241 acgggctgga aatgcgccgc aaatatgatg aatccggccg tctgattcag gaaaccgccc
      301 ctgacggcga tatcacccgc taccgttatg ataatccaca cagtgactta ccctgcgcaa
      361 cggaagatgc caccggcagc cggaaaacca tgacatggag ccgttacggt cagttgctga
      421 ccgtcactga ctgttccggt tatgtaaccc gttatgacca tgaccgtttt ggtcaggtga
      481 cggcggtgca ccgcgaggaa gggctgagcc agtaccgcgc atacgacagc cgtggacagt
      541 taattgcggt gaaagacacg cagggccatg aaatgcggta tgaatacaac gccgccggtg
      601 acctgaccgc cgtcattgcc ccggacggca gcagaaacgg gacacagtac gatgcgtggg
      661 gaaaagccat ctgtaccacg cagggcgggc tgacgcgcag tatggaatac gatgctgccg
      721 gacgggtcat ccgtctgacc agtgaaaacg gcagccacac caccttccgt tacgatgtac
      781 tcgaccggct gatacaggaa accggctttg acggccgcac acagcgttat caccacgacc
      841 tgaccggcaa acttatccgc agcgaggatg aggggctggt cacccactgg cactatgacg
      901 aagcagaccg cctcacgcac cgcaccgtga agggtgaaac cgcagagcgg tggcggtacg
      961 acgaacgcgg ctggctgaca gacatcagcc atctcagcga agggcaccgg gtgacggtgc
     1021 attacgggta tgatgagaaa ggccggctga ccggtgagcg tcagacggtg catca
//
LOCUS       DADMKF010000364         1072 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.369, whole genome shotgun sequence.
ACCESSION   DADMKF010000364 DADMKF010000000
VERSION     DADMKF010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1072
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.369"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            75..>1072
                     /locus_tag="JNC24_004340"
     CDS             75..>1072
                     /locus_tag="JNC24_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HAY9160900.1"
                     /translation="MSAYLRYIVQKADSSFLYDKYQNQSIAAHVMRALAAEQSEVSPE
                     QRRAICEAFESANNTHGLNLTAHKYPGLRGTLQTASTDCDTIVEAAALLPAFDQAVEG
                     NRHQDDYGSGLGMAEEKFHYYLDLNDRYVYFYEPVNVEYFAMNNWSFLQSGSIGIDRK
                     DIEKVFTGRTVLSSIYQDQRTKQNVMSLLTPVYVAGQLKGIVLLDINKNNLRNIFYTH
                     DRPLLWRFLNVTLTDTDSGRDIIINQSEDNLFQYVSYVHDLPGGIRVSLSIDILYFIT
                     SSWKSVLFWILTALILLNMVRMHFRLYQNVSRENISDAMTGLYNRKILTPELEQRL"
BASE COUNT          270 a          251 c          248 g          303 t
ORIGIN      
        1 attttatcgc cgcaagtgtc attgtgctga catcttcctt ccttattttt gaacttgtcg
       61 ccagcgacag agcaatgagt gcctatctgc gctatatcgt gcagaaagca gactcctcct
      121 ttctttatga taagtatcag aatcagagta ttgccgcgca tgtgatgcgc gctctcgctg
      181 ctgagcagtc ggaagtgtcg ccagaacagc ggcgcgccat ctgcgaggct tttgagtctg
      241 ccaataatac ccatggctta aacctgactg cccataaata cccgggctta cgcggcacac
      301 tacaaaccgc atccactgac tgcgacacaa ttgtggaagc tgcagcacta ttacccgctt
      361 ttgatcaggc agtggaaggc aaccgccacc aggatgatta cggttcaggt cttgggatgg
      421 ccgaagagaa atttcactat tatctcgatc tcaatgaccg ctatgtctat ttttatgagc
      481 cggttaatgt tgaatacttt gcgatgaata actggtcctt cctgcagtca ggaagtattg
      541 gcatcgatcg caaagatatt gaaaaggtat ttaccgggcg taccgtattg tcgagcattt
      601 accaggatca gcgtactaaa cagaacgtga tgagtttgct gacgccggta tatgtcgcag
      661 ggcagctaaa agggattgtg ctgctggata ttaacaaaaa caatttgcgg aatatctttt
      721 acactcatga ccgccctctc ctctggcgtt ttctcaatgt cacgctaacc gataccgatt
      781 cggggcgcga cattatcatc aaccagagcg aagataatct gttccagtat gtcagttacg
      841 tccatgactt accgggcggc attcgtgtct cgttatccat tgatattctt tactttatca
      901 cgtcttcgtg gaaaagcgtt ctgttctgga ttttgacggc gttaattttg ctgaatatgg
      961 tccgaatgca cttccgttta taccaaaatg tgtcgcgaga aaatattagt gatgcgatga
     1021 ctggactgta taatcgcaaa attttaaccc ctgaactgga gcagcggttg ca
//
LOCUS       DADMKF010000365         1059 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.370, whole genome shotgun sequence.
ACCESSION   DADMKF010000365 DADMKF010000000
VERSION     DADMKF010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1059
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.370"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(<1..>1059)
                     /locus_tag="JNC24_004341"
     CDS             complement(<1..>1059)
                     /locus_tag="JNC24_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000015417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HAY9160901.1"
                     /translation="AAQHSSQPLSALWQVLPEDVRLSPHVYLATNSLQGPWWILSWPE
                     RVPGADEVLPPEPPAYRVLTGVVDGFGRTLAFHRAAEGDVAGAVTGVMDGAGRRFHLV
                     LTTQAQRAEEARKPHTASLSSPDSPCPLSAPSFPDTLPAGTEYGADNGIRLEAVWLTH
                     DPAYPDEQPTAPLARYTYTAGGELRAVYDRSGMQVRGFTYDAEHAGRMVAHHYAGRPE
                     SCYRYDDTGRVTEQVNPEGLDYRFEYGESRVIITDSLNRREVLYTEGEGGLKRVVKKE
                     HADGSITRSEYDEAGRLKAQTDAAGRRTEYRLHMASGKLTSVILPDGRTVRYGYNSQR
                     QVTSVTYPDGLRSSREYDE"
BASE COUNT          164 a          409 c          294 g          192 t
ORIGIN      
        1 ctcatcatac tcccggctgc tgcgaagccc gtccgggtac gtcaccgacg tcacctgccg
       61 ctggctgtta tagccatacc gtaccgtcct gccgtccggc agtatcactg aagtcagctt
      121 tccagacgcc atatgcagtc ggtactccgt ccgccgtccc gccgcatccg tctgcgcctt
      181 cagcctcccc gcctcatcat actcgctgcg ggtgatgctc ccgtcggcat gctccttctt
      241 caccacccgc ttcaggccac cctcgccttc cgtgtacagc acctcccgcc ggttcaggct
      301 gtccgtgatg attacccggc tctctccgta ctcaaagcgg tagtccagcc cctccgggtt
      361 gacctgctcc gtcacccgac cggtatcatc ataccggtag cagctctccg gcctccccgc
      421 ataatggtgc gccaccatcc gcccggcgtg ctccgcatca taagtaaacc cgcgcacctg
      481 catcccgctg cggtcataca ccgcccgcag ttcaccaccg gccgtgtacg tgtagcgcgc
      541 cagcggcgca gtcggctgtt catccgggta tgccgggtcg tgtgtcagcc ataccgcctc
      601 cagccggata ccgttatcgg caccgtactc tgtaccggcg ggcagcgtgt cggggaagga
      661 aggagctgag agagggcagg gactgtcagg ggaagataac gaagcggtgt gcggtttacg
      721 ggcctcttcc gcccgctgcg cctgtgtggt cagcaccagg tgaaaacggc ggcccgcgcc
      781 gtccatcacc cccgtcaccg cgcccgccac gtcaccctcc gctgcccggt gaaaggccag
      841 cgtccgcccg aagccgtcca ccacgcccgt cagcacccgg tatgcgggcg gctccggcgg
      901 cagcacctcg tccgcccccg gcacccgctc cggccagctg agtatccacc acggcccctg
      961 aaggctgttt gtcgccaggt acacatgcgg actcaggcga acatcttccg gcagcacctg
     1021 ccagagcgcg cttagcggct gcgaactgtg ctgcgccgc
//
LOCUS       DADMKF010000366         1045 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.371, whole genome shotgun sequence.
ACCESSION   DADMKF010000366 DADMKF010000000
VERSION     DADMKF010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1045
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.371"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            284..388
                     /locus_tag="JNC24_004342"
                     /pseudo
     CDS             284..388
                     /locus_tag="JNC24_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            728..985
                     /locus_tag="JNC24_004343"
     CDS             728..985
                     /locus_tag="JNC24_004343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm development YmgB/AriR family protein"
                     /protein_id="HAY9160902.1"
                     /translation="MHSDYYNMVFGEKLANILYEANSQFFYERNVIEEAVNALFCERE
                     IINNKNIIKKLMFFLSDVNHTKKDVVQSALNIIIDITSGDI"
BASE COUNT          357 a          179 c          198 g          311 t
ORIGIN      
        1 atcatacaga taattactgg ttaaccctat atagggcatt ttaaagtgtt gtgatcttac
       61 ccagcaatag tggacacgcg gctaagtgag taaactctca gtcagaggtg actcacaaga
      121 caaaacagtt tcaaccagta aaaaaacgta aacaccattc gcatgaattt cgtagttaag
      181 atctgaagct tgcagaatgc atcggtgtta ctgccggagt ccgtgaactt agtctgcatg
      241 aatcaccaca gctttacatc tgtcgcagta aacagcaaaa tcagcaaact tacttaatta
      301 tattgagtgc gattacaatc gctggcgatg gtacagttgg tgtggcggtc tcagtccgga
      361 acaatttgaa aatcagaatc tcacttaaag cggcatccat tttacaaaag atggccgtct
      421 gtgtatttgt aatgaatacc ttctacatgt ttagagtgca taatccacga caaggcgata
      481 tcctaaaaat tagctagcag gcaactttga tggcatagat aatgttttta gtaaaaaaga
      541 gttgactgaa tataatacag cctcttccta cataagaagg aatgtgctca caaaaaaaaa
      601 ccattagtct gcttcctgtt cttacaataa tcagtattat gtattttatt tatatagcaa
      661 aatatgcttg ttacacctgt cataattagg catggtaaaa ttattgactg aaccctgggg
      721 gaggaagatg cacagtgatt attataatat ggtttttggt gaaaaattgg ccaatatttt
      781 atatgaggct aactctcaat ttttttacga gagaaatgtc atagaagagg cagtcaacgc
      841 tttattctgt gagcgtgaaa taataaacaa caagaacata ataaagaagc tgatgttctt
      901 tttgtcagat gttaatcaca ccaagaaaga tgttgttcag agcgcactta atattatcat
      961 tgacattaca tcaggtgata tttgagattt tacataaaag ataatctctc aaggagtctt
     1021 atctgaaaac gaaatatata gtccc
//
LOCUS       DADMKF010000367         1042 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.372, whole genome shotgun sequence.
ACCESSION   DADMKF010000367 DADMKF010000000
VERSION     DADMKF010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1042
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.372"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(<1..906)
                     /locus_tag="JNC24_004344"
     CDS             complement(<1..906)
                     /locus_tag="JNC24_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter YfaL"
                     /protein_id="HAY9160903.1"
                     /translation="MRIIFLRKEYLSLLPSMIASLFSANGVAAAIDLCQGYDIKASCH
                     ASRQSLSGITQDWSIADGQWLVFSDMTNNASGGAVFLQQGAEFTLSPENETGMTLFAN
                     NTVTGEYYNGGAIFAKENSTLNLTDVIFSGNVAGGYGGAIYSSGTNDTGAVDLSVTNA
                     MFRNNIANDGKGGAIYTINNDVYLSDVIFDNNQAYTSTSYSDGDGGAIDVTDNNSDSK
                     HPPGYTIINNTAFTNNTAEGYGGAIYTNSATAPYLIDISVDDSYSQNGGVLVDENNSA
                     AGYGDGPSTAAGGFMYLGLSEVTFDI"
BASE COUNT          293 a          238 c          209 g          302 t
ORIGIN      
        1 aatatcaaag gtaacttcac ttaagccgag atacataaag ccacccgccg cagtggaagg
       61 accatctcca tagcctgctg cgctgttgtt ctcatccact aacacgccgc cgttctggct
      121 gtagctgtca tcaacagaaa tatcaataag atagggagcc gtcgcgctat tggtatatat
      181 cgccccgcca taaccttcgg cagtgttatt tgtaaaggca gtgttattta ttatcgtata
      241 acctggagga tgcttgctgt cgctattatt atcggtaaca tcgattgccc cgccatcgcc
      301 atcactgtaa cttgttgatg tatatgcctg gttgttatca aaaataacat cacttaaata
      361 gacgtcatta ttaatggtat aaattgcgcc acctttgcca tcattagcga tattattgcg
      421 aaacatggcg ttagtgacac ttaaatcgac ggcaccagta tcgttagtac cagaagaata
      481 gattgcgcca ccatagccgc ctgcgacgtt accggaaaaa atgacatcag taagattcag
      541 cgttgagttt tctttagcaa atatggcccc gccgttataa tattctcctg taacggtgtt
      601 attggcaaac agagtcattc cagtttcgtt ttctggtgat aatgtaaatt ccgctccttg
      661 ttgcaaaaat acggccccac cgctggcatt attggtcata tccgaaaaaa ccagccattg
      721 cccatcggcg atactccagt cctgcgtaat gcctgaaagg ctttgcctgc tggcgtgaca
      781 actcgctttg atatcatatc cctggcataa atcaatggcc gccgcgacac cgttagcaga
      841 gaaaagagat gcaatcattg acgggagtag agataaatac tccttgcgta gaaagataat
      901 ccgcatatta atctgaccat cattttatat aagacggcat atttaaacgc attatacaat
      961 atgggttatc tttcttttgt aaattgtcca acccgatgca atggctattt tgttattatt
     1021 tgatgaataa tatcagtgcg tc
//
LOCUS       DADMKF010000368         1041 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.373, whole genome shotgun sequence.
ACCESSION   DADMKF010000368 DADMKF010000000
VERSION     DADMKF010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1041
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.373"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(88..>1041)
                     /locus_tag="JNC24_004345"
     CDS             complement(88..>1041)
                     /locus_tag="JNC24_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000445018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9160904.1"
                     /translation="IAQQKAKTEKQNQRAQAAAARAQARQQQQIAARKEKAELKLLDV
                     SSSISAMHRLSVAEQYKAIAQAREIAKQYEQGAISLARMNSQMKRLQQQQRKINGNRK
                     AQFAPVKGGSGNSATMGALLFGGATAAAGVMAVSKTSEFMANSFANAETEGELIQRAR
                     LGGVDYNLLNNLERWAYDNGIDSLQGDPGRRKILDAIGKDVKERATKSYDEAEYVVDK
                     KTGKGIWKGGDSGINTLMNEGFLTKKDLKDFANNSAGLISKAVNGMVKKGYTDAQIGS
                     RLEDLADDLLLTSKYWQRSAREVQASITQQKAAGQWLTETQ"
BASE COUNT          223 a          254 c          209 g          355 t
ORIGIN      
        1 tgtactccaa tgactagtct aaaaactagt attaagacta tcacttattt aagtgatact
       61 ggttgtctgg agattcaggg ggccagtcta ctgtgtttct gttagccatt gtccggcggc
      121 tttttgctgg gtaatgcttg cctgcacctc tctggctgat ctttgccaat atttagaagt
      181 taagaggagg tcgtccgcta aatcttccag acgactaccg atctgtgcgt cggtatagcc
      241 ctttttcacc attccgttga cagcttttga aattaaacca gcagagttat tggcaaagtc
      301 tttcaaatcc tttttagtta agaatccttc attcattaaa gtgttaatac cagaatcacc
      361 gcccttccat atgcctttcc ctgttttctt atcaactaca tattcagcct cgtcatagct
      421 tttggtagcg cgttccttta catctttacc gatggcatct agtatttttc tgcgtcccgg
      481 atcaccctgg aggctatcga tcccgttgtc atacgcccaa cgctcaagat tattaagtaa
      541 gttgtaatca actccaccaa gccttgcacg ttggattagc tccccttctg tttcagcatt
      601 agcaaagctg ttagccataa attcagaggt tttactaaca gccatcacgc cagcagcagc
      661 cgtagcgcct ccaaataaca gagcgcccat cgtggcggaa ttccctgatc ctcccttaac
      721 gggggcaaat tgagcttttc tgttgccatt aatcttacgt tgttgctgtt gtaggcgttt
      781 catctgacta ttcatacgcg ccaggctgat agccccttgc tcatattgtt ttgctatctc
      841 tctagcctgt gcaatagctt tgtattgttc cgctacagag agacggtgca ttgcactgat
      901 agagcttgat acatcaagga gttttaattc tgctttctct ttacgtgctg ctatttgttg
      961 ctgttgcctt gcctgtgctc ttgctgcggc agcttgtgct ctttgattct gcttttctgt
     1021 tttagctttc tgttgagcaa t
//
LOCUS       DADMKF010000369         1026 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.374, whole genome shotgun sequence.
ACCESSION   DADMKF010000369 DADMKF010000000
VERSION     DADMKF010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1026
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.374"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(<1..>1026)
                     /locus_tag="JNC24_004346"
     CDS             complement(<1..>1026)
                     /locus_tag="JNC24_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Rhs"
                     /protein_id="HAY9160905.1"
                     /translation="AAQHSSQPLSALWQVLPEDVRLSPHVYLATNSLQGPWWILSWPE
                     PPAYRVLTVVVDGFGRSLTFHRAAEGDVAGAVTGVTDGAGRRFHMALSTQAQRAEASR
                     KQRASSLSSPASPRSVSSSQVFPDTLPAGTEYGADNGIRLEAVWLTHDPAYPDEQPTA
                     PLARYTYTAGGELRAVYDRSGTQVRGFTYDAEHAGRMVAHHYAGRPESRYRYDDTGRV
                     TEQVNPEGLDYRFEYGESRVIITDSLNRREVLYTEGEGGLKRVVKKEHADGSITRSEY
                     DEAGRLKAQTDAAGRRTEYRLHMASGKLTSVVLPDGRTVRYGYNNQLQLTSVTYPDGL
                     RSSRKYDR"
BASE COUNT          172 a          381 c          283 g          190 t
ORIGIN      
        1 acggtcatat ttccggctgc tgcgtaaacc atccggatat gtcactgacg tcagttgcaa
       61 ctggttgttg taaccatacc gtaccgtcct gccgtccggc agtaccactg aagtcagctt
      121 tccagacgcc atatgcagcc ggtactccgt ccgccgcccc gccgcatccg tctgcgcctt
      181 cagcctcccc gcctcatcat actcgctgcg ggtgatgctc ccgtcggcat gctccttctt
      241 caccacccgc ttcaggccac cctcgccttc cgtgtacagc acctcccgcc ggttcaggct
      301 gtccgtgatg attacccggc tctctccgta ctcaaagcgg tagtccagcc cctccgggtt
      361 gacctgctcc gtcacccgac cggtatcatc ataccggtag cggctctccg gcctccccgc
      421 ataatggtgc gccaccatcc gcccggcgtg ctccgcatca taagtaaacc cgcgaacctg
      481 cgtcccgctg cggtcataca ccgcccgcag ttcaccaccg gccgtgtacg tgtagcgcgc
      541 cagcggcgca gtcggctgtt catccgggta tgccgggtcg tgtgtcagcc ataccgcctc
      601 cagccggata ccgttatcgg caccgtactc tgtaccggcg ggcagcgtgt cggggaaaac
      661 ctgagaagag gaaacagaac ggggactggc cggggaagat aaggaagagg cgcgctgttt
      721 acgggacgct tccgcccgct gcgcctgcgt gctcagcgcc atgtgaaaac ggcggcccgc
      781 gccgtccgtc acccccgtca ccgcgcccgc cacgtcaccc tccgccgccc ggtggaaagt
      841 cagtgagcgc ccgaagccat ccaccacgac cgtcagtacc cggtatgcgg gcggctccgg
      901 ccagctgagt atccaccacg gcccctgaag gctgtttgtc gccaggtaca catgcggact
      961 caggcgaaca tcttccggca gcacctgcca gagcgcgctt agcggctgcg aactgtgctg
     1021 cgccgc
//
LOCUS       DADMKF010000370         1023 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.375, whole genome shotgun sequence.
ACCESSION   DADMKF010000370 DADMKF010000000
VERSION     DADMKF010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1023
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.375"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(77..865)
                     /locus_tag="JNC24_004347"
     CDS             complement(77..865)
                     /locus_tag="JNC24_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAY9160906.1"
                     /translation="MSIIEVTGNPRHDQLVHLIAERGYMNIEELAQLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRVSSVMNTAFEQRELSLTAEKRAIAEAVADYLPERCTVFI
                     TIGTTVEAVARALLNRRDLRIITNSLRVAQILYKNQDIEVMVPGGTLRAHNGGIIGPG
                     AVDFIEGFRADYLITSIGAIEHDGTLLEFDVNEALVARTMIKHARNTLLVADHTKFAA
                     SAAVSIGNARNVRAFFTDAPPPNSFCQLLSEENVELVVAEQEVS"
     gene            complement(905..>1023)
                     /locus_tag="JNC24_004348"
     CDS             complement(905..>1023)
                     /locus_tag="JNC24_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HAY9160907.1"
                     /translation="KEAIRFASAVAAMKCTQPGGRAGIPNREQTESFLSLYA"
BASE COUNT          255 a          263 c          239 g          266 t
ORIGIN      
        1 atcgctggca ggtttttttt gcttatggaa aacaaaatcc gctgaaattc ccagcggatt
       61 ttccgaaatt tcaatttcag gatacttctt gctcggcaac caccagttca acattctctt
      121 cacttaacaa ctggcagaaa gaattgggag gcggggcatc agtaaaaaaa gccctgacat
      181 tccgtgcatt gccaattgaa acggcagcag acgcggcaaa cttcgtatga tcggccacta
      241 acaatgtatt ccgcgcatgt ttaatcatcg ttctcgcgac taacgcttca tttacatcaa
      301 attccagtag ggtgccgtca tgttcaatgg cacctatact ggtgattaaa taatctgcgc
      361 ggaagccttc aataaaatcc acggctcctg ggccgataat cccgccgtta tgagcgcgta
      421 aagtgcctcc cggcaccatc acttcaatat cctgattctt ataaagaatc tgtgccacac
      481 gtaggctgtt agtgataatg cgtaaatcac gccggttgag taatgccctg gcaacggctt
      541 ctacggttgt accgatggtg ataaagacgg tacagcgttc gggaaggtaa tcggcgactg
      601 cctcagcgat cgcccgtttc tcggcggtga gcgaaagttc ccgttgctca aaagcggtat
      661 tcatgacgct ggagacgcgc ccggcccctc catgatgacg ggtgatcaag ccctgctcac
      721 tgagttttcg gatatcccgg cgcacggttt gcgttgaaac atccagcagt tgtgccagtt
      781 cttcaatatt catataaccg cgttctgcga ttagatgaac gagttggtca tggcgtggat
      841 tacctgtcac ttcgatgata ctcatgaata ctcccttgcc cctcctcggt agatgtttac
      901 cattttacgc ataaagtgac aaaaatgatt cggtttgctc acgattagga atgccggccc
      961 gaccgccagg ctgggtacat ttcatcgcgg caaccgcact ggcaaaacga atagcctctt
     1021 ttg
//
LOCUS       DADMKF010000371         1000 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.376, whole genome shotgun sequence.
ACCESSION   DADMKF010000371 DADMKF010000000
VERSION     DADMKF010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1000
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.376"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            <1..>1000
                     /locus_tag="JNC24_004349"
     CDS             <1..>1000
                     /locus_tag="JNC24_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasin"
                     /protein_id="HAY9160908.1"
                     /translation="SGNLIEGLTVYFALKSGSATLTSLTAVTDQNGIATTSVKGAMTG
                     SVTVSAVTTAGGMQTVDITLVAGPADASKSVLKNNRSSLKGDFTDSAELHLVLHDISG
                     NPIKVSEGLEFVQSGTNVPYVQVSAIDYSKNFSGEYKATVTGGGEGITTLIPVLNGVH
                     QAGLSTTIQFTRAEDKIMSGTVSVNGTDLPTTTFPSQGFTGAYYQLNNDNFAPGKTAA
                     DYEFSSSASWVDVDATGKVTFKNVGSNSERITATPKSGGPSYVYEIRVKSWWVNAGEA
                     FMIYSLAENFCSSNGYTLPRANYLNHSSSRGIGSLYSEWGDMGHYTTEAGFQSNMYW"
BASE COUNT          293 a          221 c          253 g          233 t
ORIGIN      
        1 agtggtaacc tgatcgaagg tctcactgtg tactttgcct taaaaagcgg ctctgccaca
       61 ttaacgtcat taacagcggt gaccgatcaa aacggaatcg cgacaacaag cgtgaaagga
      121 gcgatgacag gtagcgtcac ggtaagcgca gtcacgaccg ctggtggaat gcaaacagta
      181 gatataacgc tggtggcagg cccagcagac gcctcaaagt cagtccttaa gaacaatcgg
      241 tcatcattga aaggagactt taccgacagt gctgagctac atcttgttct gcacgatata
      301 tcaggcaatc cgatcaaagt ttctgaaggg ctggaatttg tacagtcagg taccaacgtg
      361 ccctatgtgc aagttagtgc aattgactac agtaaaaatt tctcaggcga gtacaaagcc
      421 actgttacag gaggcggaga gggtatcaca acgctgatcc ctgtattgaa tggtgttcat
      481 caagctggtc tgagtaccac aatacaattc actcgcgcag aagacaaaat aatgagcggt
      541 acagtatcag tcaatggtac tgacctaccg acaactacat tcccttcgca ggggttcacc
      601 ggggcgtatt atcagttgaa taatgacaac tttgccccag gaaaaacggc ggctgattat
      661 gagttttcaa gctctgcctc ctgggtcgat gttgatgcta ccggtaaagt gacatttaaa
      721 aatgtcggca gcaattcgga aaggattacg gcgacaccaa aatcaggagg ccctagctat
      781 gtatacgaaa tccgtgtgaa gagttggtgg gtgaacgccg gcgaggcttt catgatatac
      841 agccttgctg aaaatttttg cagcagcaat ggctacacgc tccccagagc aaactattta
      901 aaccacagta gttcccgagg catcgggtca ctgtacagtg aatggggaga tatggggcat
      961 tacacgactg aagctggttt tcaatcaaat atgtattggt
//
LOCUS       DADMKF010000372          998 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.377, whole genome shotgun sequence.
ACCESSION   DADMKF010000372 DADMKF010000000
VERSION     DADMKF010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..998
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.377"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            44..904
                     /locus_tag="JNC24_004350"
     CDS             44..904
                     /locus_tag="JNC24_004350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005155803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4329 domain-containing protein"
                     /protein_id="HAY9160909.1"
                     /translation="MGRLRTQTDAAGRTTEYSPDVVTGLITRITTPDGRASAFYYNHH
                     SQLTSATGPDGLEIRREYDEWGNLLNEENPHQLQQLIRLPGQQYDEESGLYYNRHRYY
                     DPLQGRYITQDPIGLKGGWNLYTYPLSPVNSMDPLGLYEFKSKNIDDIGIFALAMCNG
                     ESINENKEYGGLICKKQGEYFPMNPISSNDNDSVDLRNIKCPEGSERVGDYHTHGFYS
                     DDKGNKVTKENDVYDSLNFSSKDLTNSYMNGMGKKEYSSYLGTPNNTYLKYNPKAKGN
                     GVTIIRQGSN"
BASE COUNT          325 a          182 c          249 g          242 t
ORIGIN      
        1 ggaacacgcg gacggcagcg tcacgcagag tcagtttgac gccgtgggca ggctcaggac
       61 acagacggat gccgcaggcc ggacaacaga atacagcccg gatgtggtga cgggtctcat
      121 cacgcgcatc accacgccgg atggcagggc atcggcgttt tactataacc accacagcca
      181 gttaacgtca gccaccgggc ctgacgggct ggaaatacgc cgggaatatg atgaatgggg
      241 caacctgctg aatgaagaga acccgcatca gctgcagcag cttattcgtt tgccggggca
      301 gcagtatgat gaggagtccg gcctgtatta taaccgccac cgctattatg acccgctgca
      361 ggggcggtat atcactcagg atccgattgg gctgaagggg ggatggaacc tgtatacata
      421 tccgctgagc ccggtgaata gcatggatcc attaggatta tatgaattta aatcaaaaaa
      481 tattgatgat attggaatat ttgcattggc aatgtgtaat ggagaatcaa ttaacgagaa
      541 taaagaatat ggtggactaa tatgtaagaa gcaaggtgaa tatttcccca tgaatccgat
      601 aagttcaaat gataatgata gtgtagactt gcgaaatata aaatgccctg aaggttcaga
      661 gagagtaggc gattatcaca ctcacggttt ttactctgac gataaaggaa ataaagtaac
      721 aaaagaaaat gatgtttatg atagtctaaa tttttcaagc aaagatttaa cgaattctta
      781 tatgaatgga atgggaaaaa aagaatacag tagttacttg ggaacaccaa ataacaccta
      841 tctaaaatat aatcccaaag ctaaagggaa tggagttaca attatcaggc aagggagtaa
      901 ttaaaatgaa ggcaatacaa tgttgttatt tccttgcttt agtctccagt catttaatgg
      961 ctggagttat gggtgatgat aatatatttt taagtaat
//
LOCUS       DADMKF010000373          983 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.378, whole genome shotgun sequence.
ACCESSION   DADMKF010000373 DADMKF010000000
VERSION     DADMKF010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..983
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.378"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            1..983
                     /locus_tag="JNC24_004351"
                     /pseudo
     CDS             1..983
                     /locus_tag="JNC24_004351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611435.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS2 family transposase"
BASE COUNT          240 a          242 c          298 g          203 t
ORIGIN      
        1 agtcttactg ctgtcgccgc cggagaacag gttgttcctg cctctgaact tgctgccgcc
       61 atgaagcaga ttaaagaact ccagcgcctg ctcggcaaga aaacgatgga aaatgaactc
      121 ctcaaagaag ccgttgaata tggacgggca aaaaagtgga tagcgcacgc gcccttattg
      181 cccggggatg gggagtaagc ttagtcagcc gttgtctccg ggtgtcgcgt gcgcagttgc
      241 acgtcattct cagacgaacc gatgactgga tggatggccg ccgcagtcgt cacactgatg
      301 atacggatgt gcttctccgt atacaccatg ttatcggaga gctgcccacg tatggttatc
      361 gtcgggtatg ggcgctgctt cgcagacagg cagaacttga tggtatgcct gcgatcaatg
      421 ccaaacgtgt ttaccggatc atgcgccaga atgcgctgtt gcttgagcga aaacctgctg
      481 taccgccatc gaaacgggca catacaggca gagtggccgt gaaagaaagc aatcagcgat
      541 ggtgctctga cgggttcgag ttctgctgtg ataacggaga gagactgcgt gtcacgttcg
      601 cgctggactg ctgtgatcgt gaggcactgc actgggcggt gactaccggc ggcttcaaca
      661 gtgaaacagt acaggacgtc atgctgggag cggtggaacg ccgcttcggc aacgatcttc
      721 cgtcgtctcc agtggagtgg ctgacggata atggttcatg ctaccgggct aatgaaacac
      781 gccagttcgc ccggatgttg ggacttgaac cgaagaacac ggcggtgcgg agtccggaga
      841 gtaacggaat agcagagagc ttcgtgaaaa cgataaagcg tgactacatc agtatcatgc
      901 ccaaaccaga cgggttaacg gcagcaaaga accttgcaga ggcgttcgag cattataacg
      961 aatggcatcc gcatagtgcg ctg
//
LOCUS       DADMKF010000374          971 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.379, whole genome shotgun sequence.
ACCESSION   DADMKF010000374 DADMKF010000000
VERSION     DADMKF010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..971
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.379"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(<1..133)
                     /locus_tag="JNC24_004352"
     CDS             complement(<1..133)
                     /locus_tag="JNC24_004352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAY9160910.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALARQNENHE
                     "
     gene            141..572
                     /gene="tnpA"
                     /locus_tag="JNC24_004353"
                     /pseudo
     CDS             141..572
                     /gene="tnpA"
                     /locus_tag="JNC24_004353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
BASE COUNT          297 a          222 c          184 g          268 t
ORIGIN      
        1 cctcatgatt ctcattctga cgtgccagag cacgattgaa aacgaaacga catgcgcctg
       61 cgaagcgcct catttcacgc tcctgttgac caccgggtct taactggaat ttaaatgctt
      121 gcagtcgttt cacaggccaa atgatacttt ggtctgtgaa aaaagaaacc gatatccggc
      181 gtggaaggca ttgtgttttc ctgatgcatg ttcacctggt ctttgtcacc aaataccgac
      241 gtcagatttt tgaccatgac gcgacagaaa aactacgcac ttacttttca aatgtatgtg
      301 ctgattttga agctgaactg gttgaaatgg atggcgaacc agatcacgtc catttgttaa
      361 ttaactatcc tcccaaactg gcgatatcca gtctggtaaa cagcctcaaa ggcgtatcgg
      421 gtaggtgact gcgacgagat cgaccagata ttgcagtcag gtattactac aaaggcgttt
      481 tgtggagtgt tggctatttt gccagtagct gcggaggtgc gccaatatcc gtcatccgcc
      541 aatacattga acaacagcaa acacctggtt aggtggaaaa ccacgcctta tatccctgcc
      601 ctgaaggacg gggttttacg gcgcaccgga taagcctgga gattatctac accagcatgg
      661 acatcattgt actatgcccc gtacatgccc catcacatcc tcaacatcaa agtattagag
      721 ccttgcaaac cattatgcgt ctcagtttgt ctcatctatc ttacgcaaaa aaaaatagaa
      781 ctaatatcaa gcggcgctat attgcgtatt aaataaaacc acctcaatta cattaataaa
      841 tctaaacaaa catcatgcaa ataatttcca tggttatttc taggctattt aaattaaata
      901 aataaattat gagtcaatca taaataacaa cctttaatat aatttaacta ccaaatttat
      961 attcgttcac a
//
LOCUS       DADMKF010000375          970 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.380, whole genome shotgun sequence.
ACCESSION   DADMKF010000375 DADMKF010000000
VERSION     DADMKF010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..970
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.380"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            <1..>970
                     /locus_tag="JNC24_004354"
     CDS             <1..>970
                     /locus_tag="JNC24_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001572598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HAY9160911.1"
                     /translation="TGKLTQSEDEGLITLWHYDASDRITRRTVNGEPAEQWQYDDHGW
                     LTEISHLSEGHRVAVHYGYDDKGRLTGERQTVETPETGEMLWEHETGHAYSEQGLATR
                     QEPDGLPPVEWLTYGSGYLAGMKLGGTPLVEYTRDRLHRETARSFGGEAYELATAWNT
                     SGQLRSRHLNLPQLDRDYDWNDNGQLIRISGPQESREYRYSDTGRLTGVHTTAANLDI
                     DIPYATDPAGNRLPDPELHPDSTLTAWPDNRIAEDAHYVYRYDEYGRLAEKTDRIPEG
                     VIRMHDERTHHYHYDSQHRLVFYTRIQHGEPQVESRYLYDPLGRRTG"
BASE COUNT          220 a          289 c          330 g          131 t
ORIGIN      
        1 tgaccggaaa actcacgcag agtgaagatg aggggcttat caccctctgg cattacgatg
       61 cgtcggaccg tatcacccgg cgcacggtga acggtgaacc ggcggagcag tggcagtatg
      121 acgaccacgg ctggctgaca gaaatcagcc acctgagtga aggccaccgt gtggcggtcc
      181 actacggcta tgacgataaa ggccgtctga cgggcgaacg gcagacggtg gagaccccgg
      241 agacggggga gatgctgtgg gagcatgaga cggggcacgc gtacagcgaa caggggctgg
      301 cgacccgtca ggagccggac ggcctgccgc cggtggagtg gctgacgtat ggcagcggtt
      361 atcttgcggg gatgaagctg ggcggaacac cgctggtcga gtacacgcgg gaccggctgc
      421 accgtgagac ggcccgcagc ttcggcgggg aggcatatga acttgccacc gcctggaata
      481 ccagcggcca gctccggagc aggcacctga accttccgca gcttgaccgt gactacgact
      541 ggaacgacaa cggacagcta atccgcatca gtggcccgca ggagagccgg gagtaccgtt
      601 acagcgacac ggggaggctg acgggcgtcc acaccacggc agcgaacctg gatatcgata
      661 tcccgtatgc aacagacccg gcaggaaatc ggctgccgga cccggaactg cacccggaca
      721 gcaccctcac ggcgtggccg gataaccgca tcgcggaaga tgcgcactat gtctatcgct
      781 acgatgaata cggcaggctg gcggagaaga cggaccgtat cccggaaggg gttatccgga
      841 tgcacgacga gcgcacccac cactatcact acgacagcca gcaccgcctg gtgttctaca
      901 cgcggataca gcacggcgaa ccacaggtgg agagccggta cctctatgac ccgctgggcc
      961 gccggacggg
//
LOCUS       DADMKF010000376          967 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.381, whole genome shotgun sequence.
ACCESSION   DADMKF010000376 DADMKF010000000
VERSION     DADMKF010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..967
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.381"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(<1..228)
                     /locus_tag="JNC24_004355"
     CDS             complement(<1..228)
                     /locus_tag="JNC24_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3289 family protein"
                     /protein_id="HAY9160912.1"
                     /translation="MNNILLPPINIPCTLFETISLFDDFSADDMQYGDMVEQDFLSLG
                     LSDISAKVDPYRLIKYHFPGPGSINVAFSASS"
     gene            364..561
                     /locus_tag="JNC24_004356"
     CDS             364..561
                     /locus_tag="JNC24_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001301726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA"
                     /protein_id="HAY9160913.1"
                     /translation="MLIVNNKMLNLPIRKLFPTRIASSYGMNFEFMPELKWSFGYPGA
                     IIFMILAGLTPYLYFKRKNWL"
BASE COUNT          315 a          165 c          188 g          299 t
ORIGIN      
        1 acttgaggca ctaaaagcaa cattaataga acctgggcca gggaaatggt atttaataag
       61 tctgtaagga tcaacttttg ctgagatatc gcttaggccg agtgagagaa agtcttgttc
      121 taccatatca ccatattgca tatcatcagc agaaaaatca tcgaataaac taatagtttc
      181 gaaaagtgta cagggaatat taattggcgg aagtaatatg ttattcattt gcatttgcct
      241 tggttatatt taagctcaag atcaattcga gaacaaatgc tagggtaaaa aaatatttaa
      301 aaaagaagta cacttcacat tttttcatta catctctgaa gagaacctcg ttaatgattg
      361 gtcttgctca ttgttaataa caaaatgttg aatttaccga ttaggaaact ttttcctacg
      421 cggattgcct ccagctatgg catgaacttt gagtttatgc cagaactgaa gtggagcttt
      481 ggctaccctg gcgcgattat ctttatgatc cttgcgggcc tgacaccgta tctgtacttt
      541 aagcgtaaga actggctgta actatacgat gtcgctgtga gtaagttagt gtgctgacag
      601 cgacatttta gccgagggaa tcgcagataa cataattgtt gtgaataata tccatgccac
      661 gttgagttgg acgcttaatg actcacatct caccccttca attgctgcag agataataga
      721 aaaaagtgtc aaacccaatg aaatcaatac aaagtagatt gccttacatc cagtatctct
      781 tattcttttt gctccaatat tgatatttac ataggtatta ttaataacca gaataaaact
      841 caaagccagg ttgatatcat tcaaagttat taatattgac catgatgtta atgtatagat
      901 tatgaactga aagcgtgata aacgctcaag cctggatggg tagaatatat ttctgatgga
      961 tgatttg
//
LOCUS       DADMKF010000377          963 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.382, whole genome shotgun sequence.
ACCESSION   DADMKF010000377 DADMKF010000000
VERSION     DADMKF010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..963
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.382"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(29..829)
                     /locus_tag="JNC24_004357"
                     /pseudo
     CDS             complement(29..829)
                     /locus_tag="JNC24_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020218947.1"
                     /note="allows for ions and hydrophilic solutes to cross
                     the outer membrane; incomplete; partial in the middle of a
                     contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
BASE COUNT          278 a          221 c          181 g          283 t
ORIGIN      
        1 actaaatcag taagttggca gcatcaccca ttgcaagata aacattgttg gcgtcgtatt
       61 ttgcaccaaa tgtccatgct tcagctgtat caccaccagc aaatttgctg tattcattgt
      121 agtgataccc gccaagagct acttgagaat tggtacggtc agagtttgag taagcggcac
      181 ctaagctcaa accgaagtca aagtcatatg aagtggaaag accaaaacca tcaccgttct
      241 ggaagcgaac gtcttcgtga ccatttttag taccttcttg atcttctcct gcattgctgc
      301 cttcgttgtt accttgatac tgcaatgcaa agttcagacc ttccaccaga ccgaagaaat
      361 cactattgcg gtaagtagct accccatttg cacggcctgc catgaagtta tcagtccagg
      421 tataggagtc gccgccgaat acaggaagca tatctgtcca ggcttcaacg tcgtaaatca
      481 caccatagtt acgaccatag tcaaaggagc ccatctcgcc gaacttcaga cctgcaaaag
      541 caagtttgac ataagtatta tcagaacctt cagtgtcgct agcctttgtt ttggtcagtg
      601 cttgaccata accagttaac tgatctgtaa tttgggtctc acctttgaag cctaactcga
      661 tgatagtttc atcgccgtca tcgccagggt tatcggaaaa agtatgacga gcatcaactt
      721 ttccgtacag atccagttta ttgccatttt tattataaat ttcagccgca tttgctgcgc
      781 cagcaactaa taacgctggg accagcattg ccagaacttt tcttttcatt atgtattccc
      841 ttgtgattat aatcttcatg aatatatcaa taagtgccgt tatccaaaaa aaaagcacat
      901 ttggatacta ttctatgaag ttcattttat tttaaagatt gcgtgtaaca aatgtattta
      961 aaa
//
LOCUS       DADMKF010000378          961 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.383, whole genome shotgun sequence.
ACCESSION   DADMKF010000378 DADMKF010000000
VERSION     DADMKF010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..961
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.383"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            211..>961
                     /locus_tag="JNC24_004358"
     CDS             211..>961
                     /locus_tag="JNC24_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISEc20 family transposase"
                     /protein_id="HAY9160914.1"
                     /translation="MTESSDYESVQVFIGVDVGKDTHHAVAINRSGKRLFDKALPNDE
                     NKLRSLISDLKQHGQILLVVDQPATIGALPVAVARSEGVLVGYLPGLAMRRIADLHAG
                     EAKTDARDAAIIAEAARTLPHALRTLKLADEQIAELSMLCGFDDDLAAQTTQASNRIR
                     GLLTQIHPALERVLGPRLEHPAVLDLLQRYPSPEKLASLGEKKLAAQLCKLAPRLGKR
                     LAADIAQALAEQTVVVPGTNAAAVVLPRLALQ"
BASE COUNT          211 a          293 c          252 g          205 t
ORIGIN      
        1 ccttataatg gggtgttagc cagccagacc cggcatgatt actgccccca gtcgtccatg
       61 atccgggggg tgatgtcacc gggtctggtg gggcgctggt aaccgctaat aggggtcagg
      121 tcaggcactt ttgccgggac cgtctgtaac gtggatgccg gtacctgctc cccgtggtta
      181 tctggttaac ccatatacaa gggagacaga atgaccgaat ccagcgatta cgaatccgtc
      241 caggtcttta tcggcgttga tgtcggtaaa gatacgcatc acgctgttgc cattaatcgt
      301 tcaggtaaac gcctgttcga taaagcatta cccaacgacg aaaacaaact caggtcgcta
      361 atatctgacc tgaaacaaca tggtcagata ctgctggttg ttgatcagcc agctaccatc
      421 ggtgcgttac ctgtcgccgt tgcccgctca gaaggagtcc ttgtcggata cctccctgga
      481 ctggccatgc gccgcatagc cgacttacac gccggtgaag ctaaaactga tgctcgtgac
      541 gctgccatca ttgccgaagc tgcccgtacc ctgcctcacg cgctacgcac gctgaaactg
      601 gctgacgagc aaatcgccga actctccatg ctctgcggct tcgatgatga tcttgccgca
      661 cagacaacgc aggccagcaa ccgtatccgc ggccttctga cccagataca tccggcactg
      721 gagcgcgttc tcggtccgag acttgagcac ccggcggtac tcgatcttct ccagcgatat
      781 ccctcaccag aaaaactcgc ttcgctgggt gagaagaagc tggcagccca gctctgcaaa
      841 cttgcgcctc gtctgggtaa acgccttgca gcagacatag ctcaggcact ggccgaacaa
      901 accgtcgtcg ttcccggcac gaatgccgct gccgtagtac tgccacgtct ggcactccag
      961 c
//
LOCUS       DADMKF010000379          960 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.384, whole genome shotgun sequence.
ACCESSION   DADMKF010000379 DADMKF010000000
VERSION     DADMKF010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..960
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.384"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            <1..>960
                     /locus_tag="JNC24_004359"
     CDS             <1..>960
                     /locus_tag="JNC24_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY9160915.1"
                     /translation="AAVQTLREMNADNLRKVPADAPTAFIKPRWKPLVITPEGLDRKF
                     YEICALSELKNALRSGDIWVKGSRQFRDFDDYLLPAEKFAALKREQALPLAINPNSDQ
                     YLEERLQLLDEQLATVTRLAKDNELPDAILTESGLKITPLDAAVPDRAQALIDQTSQL
                     LPRIKITELLMDVDDWTGFSRHFTHLKDGAEAKDRTLLLSAILGDAINLGLTKMAESS
                     PGLTYAKLSWLQAWHIRDETYSAALAELVNHQYRHAFAAHWGDGTTSSSDGQRFRAGG
                     RGESTGHVNPKYGSEPGRLFYTHISDQYAPFSTRVVNVGVRDST"
BASE COUNT          189 a          335 c          291 g          145 t
ORIGIN      
        1 ggcagccgtg cagaccctgc gtgagatgaa cgccgacaac ctgcgcaagg tgccggccga
       61 tgcacccacg gccttcatca agccgcgctg gaagccgctg gtgatcaccc cggaaggcct
      121 cgaccggaaa ttctacgaaa tctgcgccct gtccgagctg aagaacgccc tgcgctccgg
      181 cgacatctgg gtcaagggct cgcggcagtt ccgcgacttc gacgactacc tgctgccggc
      241 cgagaagttc gccgcactca agcgcgagca ggccctgccc ctggcgatca acccgaacag
      301 cgaccagtac ctggaagagc gtttgcagct gctggacgag cagttggcca ccgtcacccg
      361 cctggccaag gacaacgagc tgcccgatgc catcctcacc gagtcagggc tgaaaatcac
      421 cccgctggat gcggcggtgc cggatcgggc gcaggcgctg atcgaccaaa ccagccagtt
      481 actgccgcgc atcaagatca ccgaactgct gatggacgtg gacgactgga cgggcttcag
      541 ccgccacttc acccacttga aggacggggc cgaggccaaa gacaggacgt tgctgctgtc
      601 cgcaatcctc ggtgatgcga tcaacctcgg gctgaccaag atggccgagt cgagccccgg
      661 cctgacctac gccaagctgt cctggctgca agcctggcac atccgcgacg aaacctattc
      721 ggcggccttg gccgagctgg tcaaccacca gtatcgccac gcctttgccg cccactgggg
      781 cgacggcacg acctcatcct ccgatggcca gcgcttccgc gcgggtggcc ggggcgagag
      841 caccgggcac gtcaacccga agtacggtag cgagccggga cggctgttct atacccatat
      901 ctccgaccag tacgcgccgt tcagcacccg cgtggtgaat gtcggcgtcc gcgattccac
//
LOCUS       DADMKF010000380          957 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.385, whole genome shotgun sequence.
ACCESSION   DADMKF010000380 DADMKF010000000
VERSION     DADMKF010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..957
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.385"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            <1..395
                     /locus_tag="JNC24_004360"
     CDS             <1..395
                     /locus_tag="JNC24_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tail tape measure protein"
                     /protein_id="HAY9160916.1"
                     /translation="INAIVKPFYERQMSKSDNPIDKVVRSKVEPTDQALTGDMNSLAA
                     VLWAMVQGKSKDVPQFSPEALNNLKGRVETFTQDLKVIKPSVNIQPQTINITTQTVLD
                     GKVIDERTTSHINRMQEDTLISSAYPEE"
     gene            569..>957
                     /locus_tag="JNC24_004361"
     CDS             569..>957
                     /locus_tag="JNC24_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9160917.1"
                     /translation="MFDTKQIRYSGKDGIYFHLRDNVESFLALTATEQMDYDSPMMVT
                     TQAMQSGQTITDNVQRAPKTISISGVVVVDSTGSVLLTRQGQLVETFISTVEQWRDQK
                     QIITIVCRDGIAITDSIITQFKASKEHT"
BASE COUNT          327 a          177 c          188 g          265 t
ORIGIN      
        1 tgattaatgc cattgtcaaa ccgttctatg aacgtcagat gagtaagtca gataatccaa
       61 ttgataaagt tgtaaggtca aaggtggaac ccacagacca agcattgact ggtgacatga
      121 actctttagc cgctgtttta tgggctatgg ttcagggcaa atcaaaagat gttccacagt
      181 tttcacctga agcattaaat aatctgaaag gtcgagtgga aacattcaca caagatttaa
      241 aggttatcaa accgtcagta aatattcagc ctcagacaat caatatcaca actcagacgg
      301 tacttgatgg aaaggttatt gatgaaagaa ccacatcgca tattaatcgt atgcaggaag
      361 ataccttgat tagctcagct tatccagagg aataaacaat gttagagcta ctaatattta
      421 ttgccacaca tggcaatcta ttaatcttta tctagtatga cgattcaact atgaatagtc
      481 gaatgctacg tgaaatcttc tgaacagata ccaacatcaa aaattagatt tcacgtagct
      541 cacgcttaac agaataggaa taacaacaat gtttgacaca aagcaaatta gatactcagg
      601 taaagatggt atctattttc accttagaga taatgtggaa tcttttcttg ccttaacagc
      661 tactgaacag atggactatg actcaccgat gatggtgaca actcaggcta tgcaatcggg
      721 gcaaactatc actgataatg ttcagagagc acctaagacc atctctatta gcggtgttgt
      781 agtggttgat tcgacgggtt cagttctatt aaccagacag gggcaacttg tagaaacctt
      841 tatttctacg gttgaacaat ggcgagacca gaagcaaatc atcactattg tttgtagaga
      901 tggaatagct atcactgata gtatcattac tcagtttaaa gcgagtaaag agcatac
//
LOCUS       DADMKF010000381          935 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.386, whole genome shotgun sequence.
ACCESSION   DADMKF010000381 DADMKF010000000
VERSION     DADMKF010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..935
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.386"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(197..>935)
                     /locus_tag="JNC24_004362"
     CDS             complement(197..>935)
                     /locus_tag="JNC24_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="permease"
                     /protein_id="HAY9160918.1"
                     /translation="GPTLTFLFVSPLLNPIIVGLMWMTFGWKVTLLYAIIAAGVSVLA
                     SIILDSLGFERHIIASKSSSANCCAPAKTSPGTTYTPIKVSCCSPAAKAIENPVVTCC
                     STKAVVSINPIKLATKDALQQFKDVLPYLLLGVLIGSFIYGFIPSAWIAAHAGADNPF
                     AIPLSAVVGIPLYIRAEAVIPLASVLMTKGMGLGALMALIIGSAGASLTEVILLKSMF
                     RMPMIAAFLTVILGMAILMGYLTQLLF"
BASE COUNT          279 a          198 c          199 g          259 t
ORIGIN      
        1 ttgcttctcc gataaaatgt cagagaagca tgattacgtg gttattgtct gttgatgact
       61 tgttgaccga gccgtcatca ttctcaatgc attagttaaa tcatttgtga tttttaagga
      121 ttatcctgtc tatgattttc tacttagaag gatatggcac taactatgca tcttatagtt
      181 tactgctggc atctatttaa aatagcaatt gagtcaaata gcccatcaag atagccatac
      241 ctaatataac cgtcaggaat gcagctatca tcggcattct gaacattgat ttaagtaata
      301 tcacttccgt caggcttgcg ccggcactgc cgatgattaa tgccattaat gctcccagac
      361 ccattccttt tgtcatcaga acagatgcca gagggataac cgcctcagcc cggatataca
      421 gcggaatacc gacaacggcg cttaatggga tggcgaaggg attatctgct cctgcatgag
      481 cggcaatcca cgctgaggga ataaagccat aaataaaaga tcctattaat actcctaaca
      541 aaagatatgg cagtacatct ttaaactgtt gcaacgcatc tttggtggct agttttatgg
      601 gattaatact tactacagct ttggtactgc aacaggtaac tacggggttc tcaatggctt
      661 tagccgctgg actacaacaa ctcactttta tgggcgtata tgtcgttccc ggcgaagttt
      721 ttgctggagc acaacaattt gctgatgagc ttttactggc aatgatatga cgttcaaacc
      781 ccagagaatc caggataata ctggcaagaa cggagacgcc ggctgcgata atcgcgtaca
      841 acaaggtgac tttccaacca aaggtcatcc acattagtcc gacgataatg ggattaagta
      901 atggtgaaac aaataaaaaa gtgagagtcg gacca
//
LOCUS       DADMKF010000382          906 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.387, whole genome shotgun sequence.
ACCESSION   DADMKF010000382 DADMKF010000000
VERSION     DADMKF010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..906
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.387"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(<1..752)
                     /gene="ipaH3"
                     /locus_tag="JNC24_004363"
     CDS             complement(<1..752)
                     /gene="ipaH3"
                     /locus_tag="JNC24_004363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase IpaH3"
                     /protein_id="HAY9160919.1"
                     /translation="MSIMLPINNNFSLSQNSFYNTISGTYADYFSAWDKWEKQALPGE
                     NRNEAVSLLKECLINQFSELQLNRLNLSSLPDNLPPQITVLEITQNALISLPELPASL
                     EYLDACDNHLSTLPELPASLKHLDVDNNQLTMLPELPALLEYINADNNQLTMLPELPT
                     SLEVLSVRNNQLTFLPELPESLEALDVSTNLLESLPAVPVRNHHSEETEIFFRCRENR
                     ITYIPENILSLDPTCTIILEDNPLSSRIRESLS"
BASE COUNT          267 a          144 c          222 g          273 t
ORIGIN      
        1 gacagagact ccctgatccg tgaggacaga ggattgtctt cgaggatgat agtgcaggtc
       61 ggatcaaggc taagtatatt ttccggaatg tatgtgatgc gattctcgcg gcaccggaaa
      121 aatatctcgg tttcctctga gtgatgattt cttacaggta cggctggtag gctttccaga
      181 agattagtac ttacatcgag cgcttccagt gattcaggta actcagggag aaatgtcagc
      241 tggttatttc ttactgagag cacttccagc gatgtaggta attcaggaag catggttagc
      301 tgattgttat ctgcattaat atattccagc aatgcaggca attcaggaag catggttagt
      361 tggttgttat ctacatcaag atgtttcaga gatgcgggta attcaggaag tgttgacagg
      421 tgattgtcac aggcgtcaag gtattccagc gatgctggca attctggtaa tgatattagg
      481 gcattctgag taatttccag aacagtgatt tgaggtggta agttgtcagg tagcgaggac
      541 agatttaaac gattcagttg aagctcactg aactgattga tgagacattc tttaagtagg
      601 gagaccgctt cattccgatt ttcaccgggg agcgcttgtt tttcccattt atcccatgct
      661 gaaaagtaat cagcatatgt accggaaata gtgttataaa aagaattttg ggacaatgaa
      721 aagttattat ttatcggtaa catgatggac attcagttaa agccgatgga caaatggctt
      781 tgttaaaaga aataagtagg agtttttatg aagttcattc catgaaatga acttcagcac
      841 ttttttatta taaaaggaca aacaaaattg gctgtaaaaa aacgatactg tttcagttaa
      901 ctgtga
//
LOCUS       DADMKF010000383          886 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.388, whole genome shotgun sequence.
ACCESSION   DADMKF010000383 DADMKF010000000
VERSION     DADMKF010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..886
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.388"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            <1..344
                     /locus_tag="JNC24_004364"
     CDS             <1..344
                     /locus_tag="JNC24_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HAY9160920.1"
                     /translation="ADGHFCGNLPNTTHFHEQTVEFPIQGEMVDIVAHGELGGDFSLV
                     PDSYVTMGPKSIMAAKNLLIIVSGAGKAQALKNVLQGPVTEDVPASVLQLHPSLIVIA
                     DKAAAAELALG"
     gene            complement(406..798)
                     /locus_tag="JNC24_004365"
     CDS             complement(406..798)
                     /locus_tag="JNC24_004365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001333812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9160921.1"
                     /translation="MSWSHYRFQDDASIEGVEFEYDEEDEFAGIKNTYPDEMLKELVE
                     RTPGYHGWQQEFWLAHCGDFCAFIGYVGWNDIKDRLDEFANLEEDCENFGIRNSDLAK
                     CLQKGGDCQGYLFRCLHCGKLRLWGDFS"
     gene            complement(795..>886)
                     /locus_tag="JNC24_004366"
     CDS             complement(795..>886)
                     /locus_tag="JNC24_004366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HAY9160922.1"
                     /translation="LGRKSLSFSKSVELHDKVIGHYLNIKHYQ"
BASE COUNT          238 a          218 c          198 g          232 t
ORIGIN      
        1 gtgcagatgg tcatttttgt ggcaatttgc cgaatacgac ccatttccat gagcaaaccg
       61 tggagttccc gatccaggga gagatggttg atattgtggc gcatggtgag ttgggcggcg
      121 acttttcgtt agtgcctgac agctacgtga cgatggggcc gaaaagcatc atggcggcga
      181 aaaacttgct gataattgtc agtggcgcag gtaaagcgca ggcactgaaa aatgtgctgc
      241 aaggtccggt taccgaagat gttccagctt ccgtgttgca gctgcatcct tcgctgatag
      301 taatcgctga taaagccgct gccgcagaat tagcgctcgg ttaagctgaa gaaataaggc
      361 cggatgtatg aattacgctc cggcctgttc tcattattta aataactacg aaaaatcacc
      421 ccacagtctc agcttgccgc agtggagaca gcggaagaga taaccctgac aatcaccacc
      481 cttttgcagg catttagcta gatcagaatt tctaataccg aaattctcac aatcttcttc
      541 aaggttggca aattcatcga ggcgatcttt tatatcattc cagcccacat agccgataaa
      601 agcacagaaa tcgccacaat gcgcaagcca gaattcctgt tgccatccat gataacctgg
      661 cgtgcgttca accaactctt tcagcatttc atcaggatat gtgttcttaa taccggcaaa
      721 ttcgtcctct tcatcatact caaattcaac accttctatg ctggcatcat cctgaaaacg
      781 gtaatgactc caacttattg atagtgtttt atgttcagat aatgcccgat gactttgtca
      841 tgcagctcca ccgattttga gaacgacagc gacttccgtc ccagcc
//
LOCUS       DADMKF010000384          877 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.389, whole genome shotgun sequence.
ACCESSION   DADMKF010000384 DADMKF010000000
VERSION     DADMKF010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..877
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.389"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            <1..>877
                     /gene="cadA"
                     /locus_tag="JNC24_004367"
     CDS             <1..>877
                     /gene="cadA"
                     /locus_tag="JNC24_004367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysine decarboxylase CadA"
                     /protein_id="HAY9160923.1"
                     /translation="NFSPIYEGKCGMSGGRVEGKVIYETQSTHKLLAAFSQASMIHVK
                     GDVNEETFNEAYMMHTTTSPHYGIVASTETAAAMMKGNAGKRLINGSIERAIKFRKEI
                     KRLRTESDGWFFDVWQPDHIDTTECWPLRSDSTWHGFKNIDNEHMYLDPIKVTLLTPG
                     MEKDGTMSNFGIPASIVAKYLDEHGIVVEKTGPYNLLFLFSIGIDKTKALSLLRALTD
                     FKRAFDLNLRVKNMLPSLYREDPEFYENMRIQELAQNIHKLIVHHNLPDLMYRAFEVL
                     PTMVMTPYAAFQKELH"
BASE COUNT          238 a          221 c          212 g          206 t
ORIGIN      
        1 caacttctca ccgatttacg aaggtaaatg cggtatgagc ggtggccgtg tagaagggaa
       61 agtgatttac gaaacccagt ccactcacaa actgctggcg gcgttctctc aggcttccat
      121 gatccacgtt aaaggtgacg taaacgaaga aacctttaac gaagcctaca tgatgcacac
      181 caccacttct cctcactacg gtatcgtggc gtccactgaa accgctgcgg cgatgatgaa
      241 aggcaatgca ggtaaacgtt tgatcaacgg ttccattgaa cgtgcgatca aattccgtaa
      301 agagatcaaa cgtctgagaa cggaatctga tggctggttc tttgatgtat ggcagccgga
      361 tcatatcgat acgactgaat gctggccgct gcgttctgac agcacctggc acggcttcaa
      421 aaacatcgat aacgagcaca tgtatcttga cccgatcaaa gtcaccctgc tgactccggg
      481 gatggaaaaa gacggcacca tgagcaactt tggtattccg gccagcatcg tggcgaaata
      541 cctcgacgaa catggcatcg ttgttgagaa aaccggtccg tataacctgc tgttcctgtt
      601 cagcatcggt atcgataaga ccaaagcact gagcctgctg cgtgctctga ctgacttcaa
      661 acgtgcgttc gacctgaacc tgcgtgtgaa aaacatgctg ccgtctctgt atcgtgaaga
      721 tcctgaattc tatgaaaaca tgcgtattca ggaactggct cagaatatcc acaaactgat
      781 tgttcaccac aatctgccgg atctgatgta tcgcgcattt gaagtgctgc cgactatggt
      841 aatgactccg tatgctgcgt tccagaaaga gctgcac
//
LOCUS       DADMKF010000385          866 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.390, whole genome shotgun sequence.
ACCESSION   DADMKF010000385 DADMKF010000000
VERSION     DADMKF010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..866
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.390"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            <1..>866
                     /locus_tag="JNC24_004368"
     CDS             <1..>866
                     /locus_tag="JNC24_004368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HAY9160924.1"
                     /translation="IPVHDTIARVVSCISPAKFHECFINWMRDCHSTDDKDVIAIDGK
                     TLRHSYDKSRRRGAIHVISAFSTMHSLVIGQIKTDEKSNEITAIPELLNMLDIKGKII
                     TTDAMGCQKDIAEKIQKQGGDYLFAVKGNQGRLNKAFEKKFPLTELNNPAHDSSAMSE
                     KSHGREEIRLHIVCDVPDELIDFTFEWKGLKKLCVAVSFRSIIAEQKKEPEMMVRYYI
                     SSADLTAEKFATAIRNHWHVENNLHWRLDVVMNEDDCKIRRGNAAELFSGIRHIAINV
                     LTNDKVFKAGLRR"
BASE COUNT          284 a          156 c          207 g          219 t
ORIGIN      
        1 attcctgttc acgataccat tgccagagtt gtatcctgta tcagtcctgc aaaatttcac
       61 gagtgcttta ttaactggat gcgtgactgc cattctacag atgataaaga cgtcattgca
      121 attgatggaa aaacgctccg gcactcttat gacaagagtc gccgcagggg agcgattcat
      181 gtcattagtg cgttctcaac aatgcacagt ctggtcatcg gacagatcaa gacggatgag
      241 aaatctaatg agattacagc tatcccagaa cttcttaaca tgctggatat taaaggaaaa
      301 atcatcacaa ctgatgcgat gggttgccag aaagatattg cagagaagat acaaaaacag
      361 ggaggtgatt atttatttgc ggtaaaagga aaccaggggc ggctaaataa agcctttgag
      421 aaaaaatttc cgctgacaga attaaataat ccagcgcatg acagttccgc aatgagtgaa
      481 aagagtcacg gcagagaaga aatccgtctt catattgttt gcgatgtccc tgatgaactt
      541 attgatttca cgtttgaatg gaaagggctg aaaaaattat gcgtggcagt ctcctttcgc
      601 tcaataatag cagagcaaaa gaaagagccc gaaatgatgg tcagatatta tatcagttct
      661 gctgatttaa ccgctgagaa gtttgcgaca gcgattcgaa atcactggca cgtggagaat
      721 aatctgcact ggcgtctgga cgtggtaatg aatgaagacg actgcaaaat aagaagagga
      781 aatgcagcag aattattttc agggatacgg cacattgcta ttaatgtttt gacgaatgat
      841 aaggtattca aggcagggtt aagacg
//
LOCUS       DADMKF010000386          825 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.391, whole genome shotgun sequence.
ACCESSION   DADMKF010000386 DADMKF010000000
VERSION     DADMKF010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..825
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.391"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(<1..516)
                     /locus_tag="JNC24_004369"
     CDS             complement(<1..516)
                     /locus_tag="JNC24_004369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HAY9160925.1"
                     /translation="MKLNIFTKSMIGMGLVCSALPALAMEAWNNQQGGNKYQVIFDSK
                     IYENAWWGFSTNCPGKAKANDATNPWRLKRTATAAEISQFGNTLSCEKSGSSSSSNSN
                     TPASNTPANGGSATPAQGTVPSNSSVVAWNKQQGGQTWYVVFNGAVYKNAWWVASSNC
                     PGDAKGNDASNP"
BASE COUNT          267 a          173 c          152 g          233 t
ORIGIN      
        1 tgggttgctg gcatcattgc ctttcgcatc acccggacag ttagaagagg ctacccacca
       61 agcattttta tataccgcac cattaaagac gacataccag gtctgaccgc cctgctgttt
      121 attccaggca actacagaag aattagacgg aacagtgccc tgtgctggtg tagccgaacc
      181 gccattagcc ggcgtattgg atgcaggcgt atttgaattt gaagaagatg agctgccgct
      241 cttttcgcag gaaagtgtat tgccaaactg actaatttca gcagctgttg cggtacgctt
      301 taaacgccac gggttagttg catcatttgc tttcgctttt cccgggcaat ttgtagaaaa
      361 accccaccag gcattttcat aaattttgct atcgaaaata acctgatatt tattaccacc
      421 ttgttggtta ttccatgctt ccattgccaa tgctggcaga gcggaacaca ccagccccat
      481 accaatcata gatttagtaa atatatttaa tttcattata atcccttgtg acgtaaaaac
      541 tgcaaaacaa aagtcctacg caggaattat ttacgtttta cgagaaccct tcaatatgaa
      601 ttaaagtgag gcattaaaaa taagaaaaaa gcctgacaaa tgaagaattt taaaaacaga
      661 aacattcata tttaaaatat taaattgaat tgatatttta aatatgaata atttattcgt
      721 tctgacagta cgaataagat atgcagtcaa caaatgcaaa aagggcgccg aagcgccctt
      781 ttcaattcaa aactaattaa cgtgtaatta gcccagaact ttagc
//
LOCUS       DADMKF010000387          815 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.392, whole genome shotgun sequence.
ACCESSION   DADMKF010000387 DADMKF010000000
VERSION     DADMKF010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..815
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.392"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            <1..>815
                     /locus_tag="JNC24_004370"
     CDS             <1..>815
                     /locus_tag="JNC24_004370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS21-like element ISSso4 family transposase"
                     /protein_id="HAY9160926.1"
                     /translation="KKLTNRCIGLTLHISASTVFEVLARFKASSLSWPLPADISHDTL
                     EKLIFPPKDTSASELVMPDMLYFDTEMRKPGVTRQLLWMEYKAQAGDKAMGYSHFCRC
                     YRKWKKTRRLSMRQEHRAGEKLFIDFCGPTVPVINPDTGEIRRVAIFVAVMGASNYTY
                     VEACEGQDMMSWLNAHSRCLTFLGGVPKLLIPDNLRSAVKKADRYEPVINDSYQALAE
                     HYGTVIIPARPRKPKDKPKAENGVLIVERWLLARIRNETFHTLRALNARLREL"
BASE COUNT          199 a          228 c          207 g          181 t
ORIGIN      
        1 taagaagctg actaaccgtt gtatcggact gacgttacac atcagcgcct caaccgtttt
       61 tgaggttctt gcccgcttca aagcctcttc tctgtcatgg ccattacctg cggatatatc
      121 ccatgacacg ctggagaaac tcattttccc gcccaaagac acctctgcgt cagagcttgt
      181 tatgcctgac atgctgtact tcgatacgga aatgagaaaa cctggcgtga cgcgccaact
      241 gttgtggatg gaatacaagg cacaggcggg tgacaaagcg atgggatact cacacttctg
      301 ccgttgctac cggaagtgga aaaaaacgcg acgcctctcc atgcgacagg agcatcgtgc
      361 tggcgaaaag ctctttatcg acttctgtgg tcctacggtc cctgttatta atccggacac
      421 cggagagata cgccgcgtgg caatcttcgt ggctgtaatg ggggcgtcca actataccta
      481 tgttgaagcg tgcgaaggtc aggacatgat gtcatggtta aatgcccaca gccgttgcct
      541 gaccttcctg ggcggcgtac cgaagctgct catccccgat aacctcagaa gcgcagtgaa
      601 aaaagccgat cgctatgagc ctgtcatcaa cgacagttat caggcgctgg ctgagcacta
      661 tggcacggtt attatcccgg cccgcccgcg caaacctaaa gataaaccaa aagcagaaaa
      721 cggcgtcctc atcgtcgagc gatggcttct tgcccgcatc cgtaatgaaa ccttccacac
      781 gctcagggcg ctgaatgcac gccttcggga gttgc
//
LOCUS       DADMKF010000388          795 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.393, whole genome shotgun sequence.
ACCESSION   DADMKF010000388 DADMKF010000000
VERSION     DADMKF010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..795
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.393"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(<1..741)
                     /locus_tag="JNC24_004371"
     CDS             complement(<1..741)
                     /locus_tag="JNC24_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HAY9160927.1"
                     /translation="MRYFILMFTFVCSFVAAQPTIVPQLQQQVTDLTSSLNSQEKKEL
                     THKLESIFNNTQVQLAVLIVPTTKDETIEQYATRVFDNWRLGDAKRNDGILIIVAWSD
                     RTVRIQVGYGLEEKVTDALAGDIIRSNMIPAFKQQKLAQGLELAINALNNQLTSQHQY
                     PTNPSESESASSSDHYYFAIFWVFAVMFFPFWFFHQGSNFCRACKSGVCISAIYLLDL
                     FLFSDKIFSIAVFSFFFTFTIFMVFTCLC"
BASE COUNT          267 a          137 c          160 g          231 t
ORIGIN      
        1 acataaacag gtaaagacca taaatatggt gaaagtaaaa aagaaggaaa atacagcaat
       61 ggaaaaaatt ttatcggaga acaggaataa atctaaaaga tagattgctg aaatacagac
      121 tccactttta catgcgcgac aaaaattact gccttgatga aaaaaccaga aagggaagaa
      181 catcactgca aatacccaaa aaatagcaaa gtaataatga tcactggaag acgctgattc
      241 actttctgaa ggattagtcg gatattggtg ttgtgaagtg agttggttat tcaaagcgtt
      301 tatagctagc tctaatccct gagctaattt ttgttgttta aatgcgggta tcatgttgct
      361 acggatgata tctccagcca gagcatcggt taccttttct tccagcccat aacctacctg
      421 gatgcggaca gtacgatctg accaggcaac aatgatcagt atcccatcat tacgtttggc
      481 atctcctaaa cgccaattgt caaaaactct tgtagcatat tgttcaattg tctcatcttt
      541 ggttgtggga acaattaata cagcaagttg cacttgggta ttattgaaaa tagattctaa
      601 cttgtgcgtc agttctttct tttcttgtga gtttaagcta ctcgtaagat ccgtaacttg
      661 ttgctgtaat tgtgggacaa tagttggttg ggctgcaaca aaggaacaca cgaaagtgaa
      721 cattagtatg aaatagcgca ttataaattt ctcattaaat ctggataaat aaattgacgg
      781 cggcatcggt aattt
//
LOCUS       DADMKF010000389          790 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.394, whole genome shotgun sequence.
ACCESSION   DADMKF010000389 DADMKF010000000
VERSION     DADMKF010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..790
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.394"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            52..790
                     /locus_tag="JNC24_004372"
                     /pseudo
     CDS             52..790
                     /locus_tag="JNC24_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          228 a          194 c          208 g          160 t
ORIGIN      
        1 gccacctgaa cagaggtgat atgctcacct cagaacaaca caggtgctcc aatgaaaaaa
       61 agaaatttta gcgcagagtt taaacgcgaa tccgctcaac tggttgttga ccagaaatac
      121 acggtggcag atgccgccaa agctatggat gttggccttt ccacaatgac aagatgggtc
      181 aaacaactgc gtgatgagcg tcagggcaaa acaccaaaag cctctccgat aacaccagaa
      241 caaatcgaaa tacgtaagct gaggaaaaag ctacaacgca ttgaaatgga gaatgaaata
      301 ttaaaaaagg ctaccgcgct cttgatgtca gactccctga acagttctcg ataatcggga
      361 aactcagagc gcattatcct gtggtcacac tctgccatgt gttcggggtt catcgcagca
      421 gctacagata ctggaaaaac cgtcctgaaa aaccagacgg cagacgggct gtattacgca
      481 gtcaggtact tgagctacat ggcatcagcc acggttcggc cggagcaaga agcatcgcca
      541 caatggcaac ccggagaggc taccagatgg gacgctggct tgctggcagg ctcatgaaag
      601 agctggggct ggtcagctgt cagcagccga ctcaccggta taaacgtggt ggtcatgaac
      661 atgttgctat ccctaactac cttgaaaggc agttcgccgt gaccgagcca aatcaggtgt
      721 ggtgcggtga tgtgacctat atctggacgg gtaagcgctg ggcgtacctc gccgttgttc
      781 tcgacctgtt
//
LOCUS       DADMKF010000390          751 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.395, whole genome shotgun sequence.
ACCESSION   DADMKF010000390 DADMKF010000000
VERSION     DADMKF010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..751
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.395"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            584..>751
                     /locus_tag="JNC24_004373"
     CDS             584..>751
                     /locus_tag="JNC24_004373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HAY9160928.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVID"
BASE COUNT          213 a          135 c          164 g          239 t
ORIGIN      
        1 cttgcttgca ccgtattgtt cttgtttttc ggttagttaa acatcgtact tcatatttga
       61 acgttctgcc ggaatgcatt atcaatagag gtaaagtcgc aaccccaaat cgtaaaggaa
      121 accgtagcac gtcgtatgca agaacgtgcc acggctggct gatggatgtt cgatagcgcg
      181 agtttgaatg aaaatcagcc ggagatgatt ttacataatt gctacggaat tattcaatac
      241 aggaattgct tgcgtctgca tggattgacc tgaaatattc ccgaaaattt ctctaaaaaa
      301 ctcgaaaaaa atggtaactg gttgaatgta ttaatatgca atggtacgtg tcagggatta
      361 aaagatgaac gtaaatttat ttaacgcatt aattttaaag ggttttattg tttgttgacg
      421 aaaacaggaa tcgtgttcgg tctcttttta tctgttaaaa gccagaagca tttccttcgc
      481 tgactttata gtcaaccata acacacactc tactgtctga gtccaacgtt ttttaacatt
      541 cttgttaaaa ttatgtgatc tttagcgcgg gaggaaaata ttgatgaaac agcctgcgcc
      601 cgtttatcag agaattgcgg gtcatcaatg gcgacatatc tggctttctg gcgatataca
      661 cggttgtctt gagcagttgc gccgcaaatt atggcattgt cgttttgatc cgtggcgaga
      721 tttacttatc tcagtgggag acgttatcga t
//
LOCUS       DADMKF010000391          751 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.396, whole genome shotgun sequence.
ACCESSION   DADMKF010000391 DADMKF010000000
VERSION     DADMKF010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..751
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.396"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            <1..>751
                     /locus_tag="JNC24_004374"
     CDS             <1..>751
                     /locus_tag="JNC24_004374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YiiG family protein"
                     /protein_id="HAY9160929.1"
                     /translation="KRNLLSSAIIVAIMALGLTGCDDKKAETETPPPANSQPAAPAPE
                     APVAKAEAKPETPAQPVVDEQAVFDEKMDVYIKCYNKLQIPVQNSLARYADWLKDFKQ
                     GPTGKESTVYGIYGISESNLAECEKGVKSAVALTPALQPIDGVAVSYIDAAVALGNTI
                     NEMDKYYTQENYKDDAFAKGKTLHQTFLKNLESFEPVAESYHAAIQEINDKRQLAELK
                     NIEEREGKTFHYYSLAVMISAKQINNLISQE"
BASE COUNT          240 a          160 c          176 g          175 t
ORIGIN      
        1 tgaaaaggaa tttattatct tccgctatta tagtcgccat catggccctc ggtctgacgg
       61 gttgtgatga taaaaaagcc gaaacagaaa cgcccccgcc tgccaatagt caacctgccg
      121 caccagctcc tgaagctccc gttgcaaaag cagaagctaa acctgaaaca cctgcgcaac
      181 cggtggtcga tgaacaagcg gttttcgacg aaaaaatgga tgtctatatc aagtgctaca
      241 acaagttaca gatccctgta cagaacagtc tggcgcgtta tgctgactgg ctgaaagatt
      301 ttaaacaggg gcctaccggt aaagagagta ctgtttatgg catctacggc attagtgaat
      361 ccaacctcgc tgagtgtgaa aaaggcgtaa aaagtgctgt ggcgttaacg cctgcgctgc
      421 aacctattga tggcgtggcg gtgagttata ttgatgctgc cgtggcgttg ggtaatacca
      481 ttaacgaaat ggataaatat tacacccagg aaaattataa agacgatgcg tttgcgaaag
      541 ggaaaacgct gcaccagaca ttcttaaaaa atctggaatc ctttgaacct gtagcagaat
      601 cttatcatgc ggcgattcag gaaattaatg ataagcgcca gcttgcggaa ctgaaaaata
      661 ttgaagaaag agaagggaaa acattccact actactctct ggcagtcatg atttcagcga
      721 aacaaattaa taacctgatt tcgcaagaga a
//
LOCUS       DADMKF010000392          743 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.397, whole genome shotgun sequence.
ACCESSION   DADMKF010000392 DADMKF010000000
VERSION     DADMKF010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..743
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.397"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(<1..396)
                     /locus_tag="JNC24_004375"
     CDS             complement(<1..396)
                     /locus_tag="JNC24_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HAY9160930.1"
                     /translation="MLTKRILFSVMLIVSPSVVASEKAHELYDSIYGGKPAPDVINTL
                     HKMAESGDIDAQSLLGWEYYQPRYDTKPDVQEAIKWFELAAKQGDREAPLALGGIYYD
                     GEQVRVDYAKAYALFNQAAQHGVNLAWSRL"
BASE COUNT          233 a          172 c          108 g          230 t
ORIGIN      
        1 caacctggac catgctaaat tcacaccgtg ctgcgcggcc tgattaaata gtgcatacgc
       61 tttggcataa tccacccgca cctgctcacc gtcgtagtag atacccccca gcgctaacgg
      121 agcttctctg tcaccttgtt tagccgctaa ctcgaaccat ttaatcgctt cctgaacatc
      181 aggtttggta tcataacgag gctgataata ttcccagcct aacaggctct gcgcgtcgat
      241 atctcccgat tcagccattt tatgcagtgt atttataaca tcgggagcag gtttaccacc
      301 gtatatgctg tcatacaatt catgcgcttt ctcgctggcc accacgcttg gtgaaactat
      361 cagcattaca gaaaataata ttcttttagt taacatgtat acctttatgt cctcactgtc
      421 atatatttcc ataataatta accaccaaat atccatttaa tgaataaata cagtgccata
      481 aaggtccaag taataagcac acatttagcc tgaaacatta tcattcatgt caccaaatcg
      541 tttatttagt gagataaaac tagcatattc gtaagataaa aaatgcacat ggcaatcaag
      601 aaatgatatt attacttttt tgatgtaaaa ctatttagtt ttaaaattca ttttttcaac
      661 atatagagca ttcattttat gaatgcaaaa aaacatacct gacacacaaa ttactctcct
      721 ctgtaatatt ttctcattaa cac
//
LOCUS       DADMKF010000393          741 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.398, whole genome shotgun sequence.
ACCESSION   DADMKF010000393 DADMKF010000000
VERSION     DADMKF010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..741
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.398"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            176..694
                     /locus_tag="JNC24_004376"
     CDS             176..694
                     /locus_tag="JNC24_004376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4154 domain-containing protein"
                     /protein_id="HAY9160931.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDALSRSGVKVNP
                     DVLMLARKKNDG"
BASE COUNT          214 a          167 c          144 g          216 t
ORIGIN      
        1 cattacccac aatacgatta aattaattaa aaccccacaa ataaattaaa cataagattt
       61 tactcattgt gttttatttc tcacattgat gacgatcgca tgaaaaaatg atattttaat
      121 gagcattctg aaaaaatatc gatgcatttc gagcgaagat ggtggggatc cctgaatgcg
      181 tttttctcac cgactgttct tacttctgat tctcctgctg acgggagcac caatactcgc
      241 tcaggagcct tctgacgttg cgaaaaacgt gcgtatgatg gtttccggta ttgtcagtta
      301 cacccgctgg cctgcgctat cagggccacc aaagttatgt atcttttcat cttcacgttt
      361 tagtaccgcc ctacaagaga atgccgcgac atctttgcct tatctgcctg tcatcattca
      421 cactcaacaa gaggcgatga tttcaggctg caatggtttt tattttggca acgagtcacc
      481 gacattccag atggaattaa ccgaacaata tccgtcaaag gcgttattat taattgccga
      541 acaaaatacc gagtgcatta ttggtagcgc cttttgtctg atcatccaca ataatgacgt
      601 cagatttgcc gtaaacctgg atgccttatc gcgtagcggt gtaaaggtca acccggatgt
      661 cttaatgctc gcacggaaga aaaatgatgg ataacgataa ttctcttaat aagcgcccca
      721 cgtttaaaag agcattacgc a
//
LOCUS       DADMKF010000394          732 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.399, whole genome shotgun sequence.
ACCESSION   DADMKF010000394 DADMKF010000000
VERSION     DADMKF010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..732
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.399"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(162..>732)
                     /locus_tag="JNC24_004377"
     CDS             complement(162..>732)
                     /locus_tag="JNC24_004377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709821.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HAY9160932.1"
                     /translation="FFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIV
                     IAGILLNVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFF
                     IAVVLGMIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQ
                     IHLLDKYRKVPPEPVHADIPVGAVSDVKA"
BASE COUNT          226 a          180 c          158 g          168 t
ORIGIN      
        1 gctttggctt gataaccgga taacaacgtg cgctttgttg atgccggatg cggcgtgaat
       61 tccttatccg gtctacaaaa tttgcaaatc caaagcatta cattaagacg tggcctgata
      121 aacatatcgc atcaggccat tttcgttagc tattgtccac gtcaggcttt cacatcactc
      181 actgcaccga ccggaatatc agcatgaaca ggctcgggcg gtactttgcg atatttatcc
      241 agcagatgaa tctggatcct gcgcagcgtg tcgccattga ggcgatagaa acggaagtag
      301 agaatcagcg ttaccatgaa aaacagagtt ggtagagcaa tcataataaa ctgcatacct
      361 aatagggctt gcgtagactg ttcaacattc ggtacatagc caatcatccc taacacaacc
      421 gcaatgaaaa aagccgcaaa ggctgaaccg cccttcacca ccatagtctg cacggagtaa
      481 gcgatacttt cacagcgtac gtgcagttta tattcaccgt aatcaacggt atctgccacc
      541 atgatgacct gtaataccca gaaaagcgcc gttcccacat tcagcaaaat acccgcaatc
      601 acaatgagga cgacgttgtg atagctcatt aatgccatta acaggagaac accacagctt
      661 aacaccggaa gaatagatgc tccggcccat aaaatgcgtc gggataatga tttaactaag
      721 cgtgggaaga at
//
LOCUS       DADMKF010000395          731 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.400, whole genome shotgun sequence.
ACCESSION   DADMKF010000395 DADMKF010000000
VERSION     DADMKF010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..731
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.400"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            <1..241
                     /locus_tag="JNC24_004378"
     CDS             <1..241
                     /locus_tag="JNC24_004378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HAY9160933.1"
                     /translation="GVGLLTGNGVNGVLKGAAVGAGVGAVTEKGRDGKNARKGAKVGA
                     AVGAVTGVLTGNGLEGAIKGAVIGGTGGAILGKMK"
     gene            245..580
                     /locus_tag="JNC24_004379"
     CDS             245..580
                     /locus_tag="JNC24_004379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HAY9160934.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
BASE COUNT          202 a          154 c          202 g          173 t
ORIGIN      
        1 aggcgttgga ttacttactg gcaatggtgt taatggcgta ctgaaaggtg cagctgttgg
       61 cgctggtgtt ggtgcagtaa cagaaaaagg ccgcgacggt aaaaatgcac gtaaaggtgc
      121 aaaagtgggt gccgccgttg gtgctgtcac tggcgttctg accggcaatg gcctcgaagg
      181 ggcgattaaa ggtgccgtga ttggcggtac gggtggtgca attctgggta aaatgaaata
      241 aggaatgatg atgaaaaaat ttgctttact ggcgggcctg tttgttttcg caccaatgac
      301 ctgggcgcag gactacaata ttaaaaacgg tttgccgtca gaaacctata ttacttgcgc
      361 agaagccaac gaaatggcaa aaactgacag cgcacaggtt gcagaaattg ttgcggtgat
      421 gggtaatgcc agcgttgcca gccgtgattt aaaaattgag caatctccgg aactgagcgc
      481 caaagtggta gaaaaactga atcaggtttg cgctaaagac ccgcaaatgc tgttgatcac
      541 ggcaattgat gacaccatgc gtgctatcgg caaaaaataa tatttaatat gttaccgcgt
      601 cgatgaattc gacgcggtat tttagctaac ggggatcaga acttatattt caggcccacc
      661 atcactgcgg tgtcattgta gccattatca cccagctgca cgcccacatt accccagaca
      721 ctggccgccg g
//
LOCUS       DADMKF010000396          731 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.401, whole genome shotgun sequence.
ACCESSION   DADMKF010000396 DADMKF010000000
VERSION     DADMKF010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..731
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.401"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(<1..>731)
                     /locus_tag="JNC24_004380"
     CDS             complement(<1..>731)
                     /locus_tag="JNC24_004380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9160935.1"
                     /translation="KAIMVNGPQFGWYAPAYTYGIGLHGAGYDVTGNTPFAYPGLVFG
                     HNGVISWGSTAGFGDDVDIFAERLLAEKPGYYLHNGKWVKMLSREETITVKNGQAETF
                     TVWRTVHGNILQTDQTTQTAYAKSRAWDGKEVASLLAWTHQMKAKNWQEWTQQAAKQA
                     LTINWYYADVNGNIGYVHTGAYPDRQSGHDPRLPVPGTGKWDWKGLLPFEMNPKVYNP
                     LSGYIANWNNSPQKDYPASDLFAFL"
BASE COUNT          194 a          195 c          155 g          187 t
ORIGIN      
        1 caaaaaggca aacagatccg aagcgggata atctttttgg ggagaattgt tccagttagc
       61 aatatatccc gacagggggt tatacacctt agggttcatt tcaaaaggca atagcccttt
      121 ccagtcccat tttcccgtac caggaacggg taatcgcgga tcatggcctg attgacgatc
      181 tggataagca ccagtatgaa cataaccaat attgccgttt acatcagcat agtaccagtt
      241 gatggtcagt gcttgtttcg ctgcctgctg tgtccactcc tgccaatttt tggccttcat
      301 ctgatgagtc caggccagca aagacgccac ctctttacca tcccatgcgc gggatttagc
      361 gtaagccgtt tgtgtcgtct ggtcagtttg gagaatgttg ccatgcaccg tacgccagac
      421 agtaaaggtc tctgcctgac catttttcac cgtaatggtt tcctcacggc ttaacatttt
      481 cacccactta ccattatgca agtagtagcc tggtttctct gccaacagcc gttcagcaaa
      541 aatatcgaca tcatcgccga aacctgccgt tgatccccag gaaatcacac cattatgacc
      601 aaaaaccagc ccaggatagg caaatggtgt attgccagtg acatcataac cagcaccgtg
      661 cagaccaata ccataagtat acgcaggcgc ataccagcca aactgcggac catttaccat
      721 gattgctttc g
//
LOCUS       DADMKF010000397          731 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.402, whole genome shotgun sequence.
ACCESSION   DADMKF010000397 DADMKF010000000
VERSION     DADMKF010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..731
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.402"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            81..239
                     /locus_tag="JNC24_004381"
                     /pseudo
     CDS             81..239
                     /locus_tag="JNC24_004381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1480 family protein"
     gene            260..535
                     /locus_tag="JNC24_004382"
     CDS             260..535
                     /locus_tag="JNC24_004382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HAY9160936.1"
                     /translation="MAGYLSWLFPRCKISPKLNGTAPHFGDEMFALVLFVCYLDGGCE
                     DIVVDVYNTEQQCLYSMSDQRIRHGGCFPIEDFIDGFWRPAQEYGDF"
     gene            complement(536..>731)
                     /locus_tag="JNC24_004383"
     CDS             complement(536..>731)
                     /locus_tag="JNC24_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine/threonine-protein phosphatase"
                     /protein_id="HAY9160937.1"
                     /translation="DVDLHQVLWSRSRLGERQKGQGITGADHFWFGHTPLRHRVDIGN
                     LHYIDTGAVFGGELTLVQLQ"
BASE COUNT          189 a          185 c          161 g          196 t
ORIGIN      
        1 cagctgatag aaacagaagc cactggagca cctcaaaaac accatcatac actaaatcag
       61 taagttggca gcatcacccg ttaaccattc cttgtaaatc tgaccccgat ttatgtatgc
      121 aactcgatgc ctgggatgct gaaaccagta tccctgccct gctaaatggc gaacactctg
      181 tcctttaccg tacccgttac gatcaacaat ctgatgcctg gattatgcgt cttgcctgat
      241 ccaaaaagaa cccgtcggga tggcgggtta tttgtcctgg ttattccccc gttgtaaaat
      301 ctctcctaaa cttaacggta cggcaccaca cttcggggat gaaatgttcg cgctggtact
      361 ttttgtttgc tacctggatg gcggttgtga agatattgtt gtggatgtct acaacacgga
      421 acagcagtgt ctttattcta tgagcgatca acggatccgc catggcggtt gttttccgat
      481 tgaggatttt atagatggtt tctggcgacc tgcacaggag tacggtgatt tttaattatt
      541 gcaattgcac aagagtcagt tcgcccccaa agacagcacc ggtatcaata taatgcaggt
      601 tgccaatatc cacgcgatgt cgcaacggtg tatgaccaaa ccagaaatga tcagcacctg
      661 taattccctg ccctttttgg cgttcaccta atcgcgagcg gctccacaag acctgatgca
      721 aatcaacgtc c
//
LOCUS       DADMKF010000398          723 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.403, whole genome shotgun sequence.
ACCESSION   DADMKF010000398 DADMKF010000000
VERSION     DADMKF010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..723
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.403"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            <1..>723
                     /locus_tag="JNC24_004384"
     CDS             <1..>723
                     /locus_tag="JNC24_004384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY9160938.1"
                     /translation="IEEHYTDTAGFTDHVFALMHLLGFRFAPRIRDLGETKLYVPQGV
                     QAYPTLRPLIGGTLNIKHVRAHWDDILRLASSIKQGTVTASLMLRKLGSYPRQNGLAV
                     ALRELGRIERTLFILDWLQSVELRRRVHAGLNKGEARNSLARAVFFNRLGEIRDRSFE
                     QQRYRASGLNLVTAAIVLWNTVYLERATQGLVEAGKPVDGELLQFLSPLGWEHINLTG
                     DYVWRQSRRLEDGKFRPLRMPGK"
BASE COUNT          132 a          240 c          236 g          115 t
ORIGIN      
        1 atcgaggagc actacaccga cacggccggc ttcaccgatc acgtctttgc cctgatgcac
       61 ctgctaggct tccgcttcgc gccgcgcatc cgcgacctcg gcgaaaccaa gctgtacgtg
      121 ccgcagggcg tgcaagccta cccgacgttg cgcccgctga tcggcggcac cctgaacatc
      181 aagcacgtgc gtgcccactg ggacgacatc ctgcgcctgg ccagctcgat caagcagggc
      241 accgtcaccg cctcgctgat gctgcgcaag ctcggcagct acccgcgcca gaacggactg
      301 gccgtggccc tgcgcgagct gggccggatc gagcgcacgc tgttcatcct ggactggctg
      361 caaagtgttg aactgcgccg ccgcgtgcat gccggcctga acaaaggtga ggcgcgcaac
      421 tcgctggcca gggcggtgtt cttcaaccgc cttggggaaa tcagggatcg gagcttcgag
      481 cagcagcgct accgggccag cggcctcaac ctggtgacgg cggctatcgt gctgtggaac
      541 acggtgtacc tggaacgcgc cacccagggg ttggtcgagg ccggcaagcc ggtggacggc
      601 gagctgctgc aattcctgtc gccgctgggc tgggagcaca tcaacctaac cggcgattac
      661 gtctggcggc agagccgcag actggaagac gggaagtttc ggcccttacg gatgcccgga
      721 aaa
//
LOCUS       DADMKF010000399          710 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.404, whole genome shotgun sequence.
ACCESSION   DADMKF010000399 DADMKF010000000
VERSION     DADMKF010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..710
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.404"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
BASE COUNT          245 a          110 c          147 g          208 t
ORIGIN      
        1 gatcgattca gatgccattt tgcagtagtc tattttataa aaaaattaag taggctaaga
       61 agtaccttag aaatcaattt taaggggtct aaaacgctct gtaacgagct aaactgtata
      121 agtggcagta agatgccttt tagactgaca ctcgcttaga gagcttccta gagcttctgg
      181 gaggattcat tagatatcgt tgtatacaga gacacagtat ctataaaggg tattaagaga
      241 ttcttagaag ttactgtaaa gaaccactaa gataagaaga gagggacaaa gaaccgaagg
      301 aaagtccctt attaagtagg agtcttaata gactgtctta atagataact taaaagcctt
      361 aaaggatatc ttccaagaag agaggaacga agaaccagag gaagtgaccg ttgaccgttt
      421 gaacagactg tttaagtaaa gcctataaag tatctattaa ttaagggcta tgtctcaata
      481 ctagcagttt gtcaatacct ttgtctttag caattcgtgc tatttattaa atatagcact
      541 ttttgttaaa acagagtatg ctttttaacc taaaaccttg tcgcttcgct ctgggtcaca
      601 tgttcttggg ttcttagtga gagttacact atcttctaag gtactcaata aaaagacgag
      661 taacaaccga agaacgagga tagaggtatt gtaataactt agaaggtgtc
//
LOCUS       DADMKF010000400          692 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.405, whole genome shotgun sequence.
ACCESSION   DADMKF010000400 DADMKF010000000
VERSION     DADMKF010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..692
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.405"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            <1..>692
                     /locus_tag="JNC24_004385"
     CDS             <1..>692
                     /locus_tag="JNC24_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAY9160939.1"
                     /translation="VDCPGHADYVKNMITGAAQMDGAILVVAATDGPMPQTREHILLG
                     RQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQYDFPGDDTPIVRGSALKALEG
                     DAEWEAKILELAGFLDSYIPEPERAIDKPFLLPIEDVFSISGRGTVVTGRVERGIIKV
                     GEEVEIVGIKETQKSTCTGVEMFRKLLDEGRAGENVGVLLRGIKREEIERGQVLAKPG
                     TIKPHTKFESEV"
BASE COUNT          161 a          166 c          205 g          160 t
ORIGIN      
        1 gtagactgcc cggggcacgc cgactatgtt aaaaacatga tcaccggtgc tgctcagatg
       61 gacggcgcga tcctggtagt tgctgcgact gacggcccga tgccgcagac tcgtgagcac
      121 atcctgctgg gtcgtcaggt aggcgttccg tacatcatcg tgttcctgaa caaatgcgac
      181 atggttgatg acgaagagct gctggaactg gttgaaatgg aagttcgtga acttctgtct
      241 cagtacgact tcccgggcga cgacactccg atcgttcgtg gttctgctct gaaagcgctg
      301 gaaggcgacg cagagtggga agcgaaaatc ctggaactgg ctggcttcct ggattcttac
      361 attccggaac cagagcgtgc gattgacaag ccgttcctgc tgccgatcga agacgtattc
      421 tccatctccg gtcgtggtac cgttgttacc ggtcgtgtag aacgcggtat catcaaagtt
      481 ggtgaagaag ttgaaatcgt tggtatcaaa gagactcaga agtctacctg tactggcgtt
      541 gaaatgttcc gcaaactgct ggacgaaggc cgtgctggtg agaacgtagg tgttctgctg
      601 cgtggtatca aacgtgaaga aatcgaacgt ggtcaggtac tggctaagcc gggcaccatc
      661 aagccgcaca ccaagttcga atctgaagtg ta
//
LOCUS       DADMKF010000401          684 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.406, whole genome shotgun sequence.
ACCESSION   DADMKF010000401 DADMKF010000000
VERSION     DADMKF010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..684
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.406"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            <1..560
                     /locus_tag="JNC24_004386"
     CDS             <1..560
                     /locus_tag="JNC24_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HAY9160940.1"
                     /translation="RFSQLESALNTQKNSIPALEKEVKALDKQMVAAQKAADAYWGKD
                     ANGKQMTREEAFKKIHQQRDEFNKQNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVP
                     IQQKRDFDINEQRRQTFLQSQKLSRKLQDDWVTLEKGQYPLTMKVSEINSKKVAILMK
                     IDDINQANERWKKDTEQLRRNGVIK"
BASE COUNT          244 a          122 c          165 g          153 t
ORIGIN      
        1 atcgctttag tcagcttgaa tctgcgttga atacccagaa gaactcgatt cctgcgctgg
       61 aaaaggaagt gaaagcgctt gataagcaaa tggtggcggc gcagaaagca gcggatgcat
      121 attggggaaa agatgcaaac ggtaagcaaa tgacccgcga agaagccttt aaaaaaattc
      181 accaacagcg tgatgagttt aataaacaga acgatagcga ggcgttcgcg gttaaatatg
      241 acaaagaggt ttatcaaccg gcgattgcgg catgtcataa acagagtgaa gagtgttatg
      301 aagtgccgat tcagcagaaa cgagatttcg atatcaacga acaacggcga cagacttttc
      361 tgcaatcaca aaaactcagc cgtaaattgc aggatgactg ggtaacgctc gaaaaagggc
      421 aatatccgtt aacgatgaaa gtttcagaaa taaacagtaa aaaagtcgcg attttgatga
      481 aaattgacga tatcaaccaa gctaatgaac gctggaaaaa agataccgaa caactgcggc
      541 gcaatggggt gattaagtaa ctcatgttaa gtgcctttgc cggataatat aaaaagttat
      601 ccggcaaatt attttttaca ttagccctag tagctgtgga atgaacaagg atatttgcgg
      661 aatgtaagtg atcatcatca acgc
//
LOCUS       DADMKF010000402          677 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.407, whole genome shotgun sequence.
ACCESSION   DADMKF010000402 DADMKF010000000
VERSION     DADMKF010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..677
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.407"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            <1..>677
                     /locus_tag="JNC24_004387"
     CDS             <1..>677
                     /locus_tag="JNC24_004387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HAY9160941.1"
                     /translation="QMQSQMDPVYTPARKIHLYHCDHRGLPLALISTEGTTAWCAEYD
                     EWGNLLSDENPHHLQQLIRLPGQQYDEESGLYYNRHRYYDPLQGRYITQDPIGLKGGW
                     NFYQYPLNPVQYIDSMGLASKYGHLNNGGYGAKPSKPPTPDPSKLPDIAKQLRLPYPI
                     DQASSAPNVFKTFFRALSPYDYTLYCRKWVKPNLTCMPQDDPQYPGMETKTASDYLPQ
                     TNWSTTQ"
BASE COUNT          194 a          170 c          171 g          142 t
ORIGIN      
        1 gcagatgcaa agccagatgg acccggtata cacgccggcg cgaaaaattc acctgtacca
       61 ctgcgaccat cgcggcctgc cgctggccct tatcagcacg gaagggacaa cagcgtggtg
      121 cgcagaatat gatgaatggg gcaacctgct gagtgatgag aacccgcatc atctgcagca
      181 gctcattcgc ctgccggggc agcagtatga tgaggagtcc ggcctgtatt acaaccgcca
      241 ccgctattat gacccgctgc aggggcggta tatcactcag gatccgattg gactgaaggg
      301 gggatggaat ttttatcagt atccgttgaa tccggttcag tatatagatt caatggggct
      361 ggcatctaaa tatggacact taaataatgg cggatatgga gcgaaaccca gcaaaccgcc
      421 tacgcccgat ccaagtaagt tgccagacat agcgaaacaa ttaagactgc catatcctat
      481 tgaccaggcc agtagtgcgc ctaatgtttt caaaacattc ttcagagcat taagccctta
      541 cgattacaca ctgtattgca ggaagtgggt aaaacccaat ctgacttgta tgccgcagga
      601 tgatccccag tatccaggga tggagacaaa gacagcaagt gattacctgc cacagacaaa
      661 ttggtcaaca actcaat
//
LOCUS       DADMKF010000403          663 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.408, whole genome shotgun sequence.
ACCESSION   DADMKF010000403 DADMKF010000000
VERSION     DADMKF010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..663
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.408"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            <1..>663
                     /locus_tag="JNC24_004388"
     CDS             <1..>663
                     /locus_tag="JNC24_004388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
                     /protein_id="HAY9160942.1"
                     /translation="LPQRSDTLDKQELNLKSDRKEKEFPRIKLNGQCYFPGRPQNRIV
                     CRHIAAQYINDIYQNVDYKPHQDDYSSAEKFLTHFNKKCKNQTLALISSRPEGRCVAA
                     CGDFGLVMKAYFDKMESNGISVMAAILLVDNHALTVRLRIKNTTEGCTHYVVSVYDPN
                     VTNDKIRIMSESKEDIKHYSLMDFMNVDYSLLKWSNDHVINQSVAIIPALPKEQLLIL
                     KG"
BASE COUNT          230 a          124 c          123 g          186 t
ORIGIN      
        1 ttttacctca acgctcagac actcttgata aacaagaatt aaacctaaaa tcagatagaa
       61 aagaaaaaga attcccaaga attaaactta atggtcaatg ttattttccg gggcgacccc
      121 aaaaccgtat agtatgccga cacattgctg cacaatatat taatgatatt tatcagaatg
      181 ttgattacaa accccatcaa gatgattatt cttcagctga aaaatttctc actcacttca
      241 acaaaaaatg caaaaaccag actttggcgt tgatttccag ccgtcctgag gggcgttgcg
      301 ttgctgcctg cggtgatttc gggctagtta tgaaagcata ttttgacaag atggaatcaa
      361 atggcatcag tgttatggca gccatattac tggtggataa ccatgctttg acggtccggc
      421 taagaataaa gaacacaact gaaggatgta cccattacgt ggtttcggtt tatgatccta
      481 atgtaactaa cgacaaaata agaatcatga gcgaaagcaa agaggatatt aaacactatt
      541 ctctgatgga ttttatgaat gtagattata gcctcctgaa atggtcaaat gatcatgtta
      601 ttaaccaatc tgttgcaata attccagcac ttccgaaaga acagctattg atattaaaag
      661 gat
//
LOCUS       DADMKF010000404          662 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.409, whole genome shotgun sequence.
ACCESSION   DADMKF010000404 DADMKF010000000
VERSION     DADMKF010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..662
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.409"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            116..>662
                     /locus_tag="JNC24_004389"
     CDS             116..>662
                     /locus_tag="JNC24_004389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HAY9160943.1"
                     /translation="MITDLILHNHPRMKTITLNDNHIAHLNAKNTTKLEYLNLSNNNL
                     LPTNDIDQLISSKHLWHVLVNGINNDPLAQMQYWTAVRNIIDDTNEVTIDLSGLNLTT
                     QPPGLQNFTSINLDNNQLTHFDATNYDRLVKLSLNSNALESINFPQGRNVSITHISMN
                     NNALRNIDIDRLSSVTYFSAAH"
BASE COUNT          247 a          133 c           89 g          193 t
ORIGIN      
        1 aattaatatt tctgatagtt ataaactgaa atttcttaat cttgattata acaagctagc
       61 atcgctggat gtctcccggc aagaatctct gattgagtta agtgcccatc acaatatgat
      121 cactgacctt atattacaca atcatcccag gatgaaaaca atcactttaa acgacaacca
      181 tattgcacat ttaaacgcca aaaacactac aaaactggaa tatttaaact taagcaataa
      241 caatttactg ccaaccaatg acattgatca actaatatca tcaaaacatc tttggcatgt
      301 attagttaac ggcatcaaca atgatccact tgcacaaatg cagtactgga ctgcagtaag
      361 aaatataatt gatgacacta atgaagtgac cattgattta tcaggactta atttaaccac
      421 tcaaccacca gggctgcaaa acttcacatc tatcaatctt gataataacc aactcacaca
      481 ttttgatgca accaactacg atagactcgt aaagctaagt ctgaatagta atgctcttga
      541 gtcaataaat tttcctcaag gcagaaatgt aagtattaca catatatcta tgaataataa
      601 tgctctcaga aatattgata tagataggct ttcatcagtt acttatttta gtgcggcaca
      661 ta
//
LOCUS       DADMKF010000405          651 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.410, whole genome shotgun sequence.
ACCESSION   DADMKF010000405 DADMKF010000000
VERSION     DADMKF010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..651
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.410"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
BASE COUNT          206 a          155 c          127 g          163 t
ORIGIN      
        1 tcggaaagaa aaaaggccat cagcaaaact ctgatggcct gaatcacctt taccaatatt
       61 gtatgaaaaa acacgcacga ctaattgaca ataattttca tttccattaa aaaagatcgc
      121 gtagcattct taattcatga ggaactttac ccgccagcaa tctgagtagc gaaagctgtt
      181 catccccaat gtttttgctg gcgggtcctt ttttctacgg tcctcctccc cggagggggg
      241 catttcatta tttttctaat cgttactggt gagaaggcgg aagaaaaagc cagttcttcg
      301 cccctaaata acagcatgat tccagtccat gcaaaatctg atttccagct cacacatacc
      361 gagcgctgta caaaaaaccg ccagccaagg cagttaagag tgtgttcccg gggtttgctt
      421 agaatatttt gtaagttgtc cgaagagata tttacaacac cagaatgatg aatcatcagt
      481 cccctgccag aggttcacta cgtaacctga ccgacaatgc accattctgg tgctgaaaaa
      541 agagcactgg aactgcaaca aaacaacatc acagaacagg agatggtaag gagtcagaca
      601 ttcccacaca atattgtgtc agtgcaaata acaaccttcg tctcaaatct c
//
LOCUS       DADMKF010000406          649 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.411, whole genome shotgun sequence.
ACCESSION   DADMKF010000406 DADMKF010000000
VERSION     DADMKF010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..649
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.411"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(487..>649)
                     /locus_tag="JNC24_004390"
     CDS             complement(487..>649)
                     /locus_tag="JNC24_004390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HAY9160944.1"
                     /translation="TEEVVNQRNIKVKNMTLDEINNTYCNNDYYNQAIREEPIDLLDR
                     SFSSSSWPF"
BASE COUNT          206 a          114 c          130 g          199 t
ORIGIN      
        1 gcgctaaata gcatccagac acagaaggca agaagtagac aactgactga tatccagagg
       61 tttcttcgag caatatgttt tcctttattt tcccagaagg ccggattttc tggtttccag
      121 tcgcgtaaaa gataacgact atttttctca ttttgcagtg ccatattgtt cctcacatgc
      181 acacattggt aatgaaaaaa gacaaaacag gaggtaaggc gcaatagcca gttattagaa
      241 ttaaggatga attgggtgaa gtgctgattg aaagaataga taagaaagcg taaccgcggg
      301 ggcagaatgg ggattaagta gccagatatg tgttactaaa tgtaactaac aagctaacct
      361 tcaaacgggg ttaatctttg aacgtatgtc atgtttcaac ccttcagatc gtgaatctaa
      421 agggttacat attaactata ttatcaacaa gaccagacac ttatagaatt agtgatgatt
      481 tattcactaa aaaggccatg aactggagga aaacgatctg tccagaaggt ctatcggttc
      541 ctctcttatt gcctgattgt aataatcgtt attacagtaa gtattgttta tttcatctaa
      601 ggtcatgttt ttaactttta tattcctctg gtttacaact tcctctgtc
//
LOCUS       DADMKF010000407          642 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.412, whole genome shotgun sequence.
ACCESSION   DADMKF010000407 DADMKF010000000
VERSION     DADMKF010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..642
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.412"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            <1..>642
                     /gene="fliD"
                     /locus_tag="JNC24_004391"
     CDS             <1..>642
                     /gene="fliD"
                     /locus_tag="JNC24_004391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HAY9160945.1"
                     /translation="VSTEAGAAPGIYTISVTQLAQAQSLRTDSPTIIASTKDALGDES
                     SDTRTIKITQDGRKEPLEIKLNKDQTSLDEISKAINDADSGISASIVKVKDGNYQLVL
                     TASEGLANKMTISVEGDSKLNDLLAYDSKTNTGNMKELVNAQNAQLNVNGIDIERSSN
                     KITDAPQGVTLDLTKKVTDVRVTVTKSNDKATEAIKGWVDSYNSLIDTFNTLT"
BASE COUNT          218 a          146 c          139 g          139 t
ORIGIN      
        1 aagtttccac cgaagcaggt gctgcccccg gtatctatac catcagcgta acccaactgg
       61 cgcaggcgca atctttacga actgattccc cgacaatcat tgcatcaaca aaagatgcac
      121 tgggtgatga gtcatcagat acacgcacaa taaaaattac ccaagacggt cgtaaagagc
      181 cgctggaaat caagctcaat aaagatcaga cgtcattgga tgagatcagc aaagccatta
      241 acgatgccga tagtggaatt tccgctagta tcgttaaagt caaagatggt aactatcaat
      301 tggtattgac cgccagtgaa ggtctggcca ataaaatgac catatctgtt gagggtgaca
      361 gtaaattaaa tgatctgctg gcttatgaca gcaagaccaa caccgggaat atgaaggaac
      421 tggttaacgc gcaaaatgca cagctaaacg ttaacggtat cgatattgaa cgttcaagta
      481 acaaaattac cgatgctcct cagggagtga ctctcgattt gaccaaaaaa gtcactgacg
      541 tccgggtcac ggtgacaaaa agcaacgata aagcaaccga agcaattaaa ggctgggtgg
      601 attcatataa ctccctcatt gacaccttta acaccctgac aa
//
LOCUS       DADMKF010000408          633 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.413, whole genome shotgun sequence.
ACCESSION   DADMKF010000408 DADMKF010000000
VERSION     DADMKF010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..633
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.413"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(<1..100)
                     /locus_tag="JNC24_004392"
     CDS             complement(<1..100)
                     /locus_tag="JNC24_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HAY9160946.1"
                     /translation="MASVSISYPSCSATDGVVRNCKSTAGHQRYLCS"
     gene            156..633
                     /locus_tag="JNC24_004393"
                     /pseudo
     CDS             156..633
                     /locus_tag="JNC24_004393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
BASE COUNT          155 a          140 c          148 g          190 t
ORIGIN      
        1 gagagcagag atagcgctga tgtccggcag tgcttttgca gttacgcacc accccgtcag
       61 tagctgaaca ggagggatag ctgatagaaa cagaagccac tggagcgcct caaaaacacc
      121 atcatacact aaatcagtaa gttggcagca tcaccgccgt tgtctacaca tttattctct
      181 cggtgctgat ttaccgggaa gtaaaatggc gccatttacc caaactgatt ctggagtcgg
      241 ttgttaccac ttcgattgta ttactgttga tcggtttttc cgtgggcatg tcctgggcaa
      301 tgactaatgc tgatattcct tatatgatca gcgacgcatt gatggggatt tctgataacc
      361 cactgatcat attgctgctg attaatatcg tcttattaat tgtcggcatc tttatggaca
      421 tgacaccggc tgtactaatc tttacaccga tcttcttgcc gattgcacaa gagctgggaa
      481 tggacccagt acatttcggt attatgatgg tggccaacct ttgtattggg ctgttaacac
      541 cgccggtggg tagtgcgcta tttgttggtt gttctatctc gggtgtgaag attcaacatt
      601 taatcaaacc attgctgcca ttttatgcta gca
//
LOCUS       DADMKF010000409          621 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.414, whole genome shotgun sequence.
ACCESSION   DADMKF010000409 DADMKF010000000
VERSION     DADMKF010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..621
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.414"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
BASE COUNT          194 a          128 c          110 g          189 t
ORIGIN      
        1 tgcctggcat agcaagccct tttcttgtgt tgtcaagaca tttggcaacg ttcaaacatc
       61 tgtttagaaa tgccaaattg ttgatctaac aaataaacct gtataaacag aacaccattt
      121 aatctattcc cctgtgtaac ccaccacatc acacaataaa aacaaaaaac tttaattata
      181 tttcatagca cacaaaagtc aatagaagcc ctttcgtgct cctacgtaaa aaatccatca
      241 cttttccact gatacatcaa ttcaaggcgt tatatgtgtt ccttagcaca tataacattt
      301 attatgttaa atggattttg gcctatattt tgacctgcac ccgcataaac actggtttca
      361 tcgcttacag cccttgtgag acgtggtgct gtttctagca tctgatccga cataaaaccg
      421 atcattctcg tgtttagtga aagtttagtg agagacatta cagaaaggct ttctgtgtaa
      481 tgatttaata ggtaaaaacg atgttgatta cgcaagaaaa ttccttgctg atatttgggg
      541 attctggatt ttccagatcg gggggcgctg aaaatcgcgt taagcacctc tcgcaatcta
      601 gccataaggg aagggatata g
//
LOCUS       DADMKF010000410          594 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.415, whole genome shotgun sequence.
ACCESSION   DADMKF010000410 DADMKF010000000
VERSION     DADMKF010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..594
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.415"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            289..>594
                     /locus_tag="JNC24_004394"
     CDS             289..>594
                     /locus_tag="JNC24_004394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAY9160947.1"
                     /translation="MPGTGKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVGKRQLDEINRIRYELELMAVALAVE
                     "
BASE COUNT          173 a          121 c          149 g          151 t
ORIGIN      
        1 cggtttagag aatgcgccac agacgtttat cggcctgctg aagggtaaaa acttcggcaa
       61 agtggtgatc cgcgtcgcgg gtgatgatta aagtttcaac ggcggcgtaa gccgccataa
      121 aaatccaggc aaaaagctta ttttcagctt taattaacta accacgtcat tgttctgctg
      181 ctaataacaa caaaggtggg tggtttatga ttattcactt taatacacca ggtgaactcc
      241 ttctgccgtg caggcagggt tggacagaaa acacgcgaag gaattaaaat gcctggaacg
      301 ggaaaaatga aacatgtcag tttgactctg caggttgaga acgacctgaa acatcagctt
      361 agtattggcg cattaaaacc tggcgcacgc ctgattacta aaaatctggc ggagcaatta
      421 ggtatgagta ttacacctgt gcgtgaagca ttattacgtc tggtttcggt gaatgcgctt
      481 tctgtcgcac ctgcacaagc atttacagtt ccggaagtgg ggaaacgtca attagacgaa
      541 atcaatcgga tccgctacga actggaatta atggcagttg ctctggctgt tgaa
//
LOCUS       DADMKF010000411          575 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.416, whole genome shotgun sequence.
ACCESSION   DADMKF010000411 DADMKF010000000
VERSION     DADMKF010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..575
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.416"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
BASE COUNT          157 a          113 c           91 g          214 t
ORIGIN      
        1 tatttatact tatcgggtat cagttttgat aggggtatca gacttgatag gggtaacaga
       61 attctgtggg ggtagttcac tatgacctac ccctagttca ctgtgagcta ccgttacatc
      121 caatcatctt ccgatgtaac gtatcgttac taccgatgtg taatatcgtt acattcaata
      181 attagtctat gtaatatatc gttactacca actttcttac agagagcatt tcagataaat
      241 gaatgtaacc tctcgttaca tccatttgtc acatagcact ctgtgtaatg tctttctatg
      301 tgtgtgccat aatcccccta tattccctgt gtaaatcata gaataatgct ctctattccc
      361 tgtgtaattc cctactaaaa aatccaatga aaagtagctt aaaagttcaa ttcttacttt
      421 cttcactttt acatcacttt cgcaaagcct tgtgtaatgc ggcttacaga gcaccatcag
      481 aaattgttgg ctaaaaattg ttctgtttgt ttctgaacga gtttctcgtt tgtgcaaatt
      541 ttaaaattta ttttgttttt atagccttat ttcgt
//
LOCUS       DADMKF010000412          566 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.417, whole genome shotgun sequence.
ACCESSION   DADMKF010000412 DADMKF010000000
VERSION     DADMKF010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..566
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.417"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            <1..225
                     /locus_tag="JNC24_004395"
     CDS             <1..225
                     /locus_tag="JNC24_004395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY9160948.1"
                     /translation="DSYDNAMAESINGLYKAEVIHRKSWKNRAEVELAILTWVDWYNN
                     RRLLERLGHTPPAEAEKAYYASIGNDDLAA"
     gene            267..467
                     /locus_tag="JNC24_004396"
                     /pseudo
     CDS             267..467
                     /locus_tag="JNC24_004396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611683.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          174 a          128 c          142 g          122 t
ORIGIN      
        1 gactcgtatg acaacgcgat ggcggagagc atcaatggtc tttacaaagc ggaggtaata
       61 caccgtaaga gctggaaaaa ccgtgcagaa gtggaactgg ccatactcac gtgggtggac
      121 tggtataaca atcgacgatt gctggaaagg ctgggccata ctcctccggc agaagcagaa
      181 aaagcttatt atgcttccat cggaaacgat gatctggcag cctgagttca cagataaaac
      241 actctccagg aaacccgggg cggttcaacc ttcacgataa aaaacttttc attaaaggta
      301 aagcggatca atatccggca cttcattctc tgatatctcc gacccgcatt aatctgaaag
      361 aaatagaaat tcactggcgt gaagtgctac gtctggcgac ctcaataaaa cagggaacag
      421 tgacagcatc cctgatgctg agaaaacttg ccagttaccc caaacaaaag taaggcagta
      481 tgactttccg tgagacagat gctggccaac gtgggatatt taacgcgggt taacatcgtt
      541 gtcagagccg catgggtgag ctgttg
//
LOCUS       DADMKF010000413          551 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.418, whole genome shotgun sequence.
ACCESSION   DADMKF010000413 DADMKF010000000
VERSION     DADMKF010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..551
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.418"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            <1..>551
                     /gene="feaR"
                     /locus_tag="JNC24_004397"
     CDS             <1..>551
                     /gene="feaR"
                     /locus_tag="JNC24_004397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator FeaR"
                     /protein_id="HAY9160949.1"
                     /translation="WQEVKGSDDAWFYTVFQLSGQAIMEQDERQVQIGAGDITLLDAS
                     RPCSLYWQESSKQISLLLPRTLLEQYFPHQKPVCAERLDADLPMVQLSHRLLQESMNN
                     PALSETESEAALQAMVCLLRPVLHQRESVQPRRERQFQKVVTLIDDNIREEILRPEWI
                     AGETGMSVRSLYRMFADKGLVVA"
BASE COUNT          139 a          125 c          146 g          141 t
ORIGIN      
        1 tggcaggaag taaaaggcag cgacgatgcc tggttttaca ccgtttttca gcttagtggt
       61 caggcaataa tggagcagga tgagcgtcag gtgcagattg gcgctggcga tattacgtta
      121 ctcgatgcct cacgcccctg ttcgctttac tggcaggagt cttctaaaca gatttcatta
      181 cttttgccac gcactctgct ggaacaatat tttccccatc aaaaacctgt ctgcgcagaa
      241 agactggacg ctgacttacc catggtgcaa ctcagtcatc gcctgttaca ggagagcatg
      301 aataatccgg cactttctga aacagaaagt gaagctgcgc tacaggcgat ggtgtgtctg
      361 ctgcgcccgg tacttcatca gcgggaatct gttcaacctc gccgtgaacg tcagtttcaa
      421 aaagtggtta cgttgataga cgataatatt cgcgaagaga tattacgccc ggagtggata
      481 gccggagaga caggtatgtc agtacgtagt ttgtaccgaa tgtttgccga taaaggtttg
      541 gtagtcgcgc a
//
LOCUS       DADMKF010000414          546 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.419, whole genome shotgun sequence.
ACCESSION   DADMKF010000414 DADMKF010000000
VERSION     DADMKF010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..546
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.419"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            <1..511
                     /locus_tag="JNC24_004398"
     CDS             <1..511
                     /locus_tag="JNC24_004398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001400703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAY9160950.1"
                     /translation="RLIKNACLKLYNLAHTATNSHLKPTGPDNSDVLLFKKLFSPSKL
                     MTIIGDEIPLISEKQSLSKVLLNDENNELSDGTNFWDKNRQLTTDEIACYLQKIAANA
                     KNTQVNYPTGLYVPYSTRTHLEDALNENIKSDPSWPNEVQLFPINTGGHWILVSLQKI
                     VNKKIINYK"
BASE COUNT          194 a          120 c           83 g          149 t
ORIGIN      
        1 ccgacttatt aaaaatgcct gtttaaaatt atataattta gcacataccg caaccaactc
       61 ccaccttaaa ccaactggtc cggataattc agatgttctc ctgttcaaaa agctattctc
      121 cccttcgaaa ttaatgacaa ttatcggtga tgaaattcct cttatatctg aaaaacagtc
      181 gctttcaaag gtgcttttaa atgatgagaa taatgaactg agtgatggta caaacttctg
      241 ggataaaaat cgtcaattaa ccacagatga aatagcttgc tatcttcaga agatcgccgc
      301 taatgcaaaa aatactcaag tcaattatcc tactggtctc tacgtcccct actccaccag
      361 aactcacctg gaagacgctc tcaatgaaaa tattaagagc gatccatcat ggccgaatga
      421 agtccagtta ttccccataa atactggcgg acactggata ttagtttcgc tacagaaaat
      481 agtaaataaa aaaataataa actacaaata aaatgcgtca tattcaactc attgcgtgca
      541 ctaggc
//
LOCUS       DADMKF010000415          543 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.420, whole genome shotgun sequence.
ACCESSION   DADMKF010000415 DADMKF010000000
VERSION     DADMKF010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..543
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.420"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            <1..>543
                     /locus_tag="JNC24_004399"
     CDS             <1..>543
                     /locus_tag="JNC24_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HAY9160951.1"
                     /translation="TILSDNYNAITLGNGWNTPLGAISFYAARSSSKLNNDTRHEGTS
                     YQVAYNKYLVQTATRFSVAAWRYASQDYRTFSDHLYENDKHYHQSDYDDFYDIGRKNS
                     LSANIMQPLSNNLGNVSLSALWRNYWGRSGNAKDYQFSYSNNWQHISYTFSASQSYDE
                     NNKEEERFNLFISIPFYWGDD"
BASE COUNT          168 a          105 c          109 g          161 t
ORIGIN      
        1 acgattttat ccgataacta taatgctata actctgggaa atggttggaa tacaccgctg
       61 ggtgccatct ctttttacgc tgcccgatcg agtagtaagt taaataatga taccagacac
      121 gaaggtacga gctatcaggt cgcctataat aaatatctag tacaaacagc cactcgattt
      181 agcgtggccg cctggcgtta tgcttcgcag gattacagga cattcagcga ccatctttac
      241 gaaaatgata aacactatca tcagagtgat tatgatgatt tttatgatat tggcagaaaa
      301 aatagccttt ctgccaatat tatgcaacct ttatccaata atctgggaaa tgtatcatta
      361 agtgcgcttt ggcggaatta ctgggggcga agtggaaatg ctaaagatta ccaattcagt
      421 tactccaata actggcaaca cattagttat actttctctg ccagccaatc ttatgatgaa
      481 aataataaag aagaggagcg ttttaatctg tttatctcca ttcctttcta ctggggggat
      541 gat
//
LOCUS       DADMKF010000416          520 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.421, whole genome shotgun sequence.
ACCESSION   DADMKF010000416 DADMKF010000000
VERSION     DADMKF010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..520
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.421"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            36..>520
                     /locus_tag="JNC24_004400"
     CDS             36..>520
                     /locus_tag="JNC24_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAY9160952.1"
                     /translation="MSSASAEDLTLLQLRRSGLVIADLAGESEDPRSVCEHYYSLISQ
                     YREIHWVFMVSRSWYSQAVELLMCPTATLLSDVEPIENLVKTVRSGNTHAERISAMLT
                     SPAMNETHDFSYRSVILTLSERKVLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRR
                     LA"
BASE COUNT          138 a          121 c          117 g          144 t
ORIGIN      
        1 aggtcatgag gactcacttc cctgaatatg aaataatatc cagcgcctct gcggaggacc
       61 ttacattatt acaattacgt cgttccggat tagtcattgc tgatttagcc ggtgaaagtg
      121 aagatccacg ttctgtttgt gaacattatt attctttaat ctcacaatat cgggaaattc
      181 actgggtttt catggtgtca cgctcctggt attcccaggc agtagaattg ctcatgtgcc
      241 ctacggcgac gttattgtct gatgttgaac ccattgagaa tctggtcaag accgtacgtt
      301 ccggcaatac gcacgcagag cgtatcagcg ccatgctgac ctctccggca atgaatgaaa
      361 ctcatgattt tagctatcgc tccgtcattc tcactctttc agagcgcaag gtactgcggc
      421 tattaggtaa aggatggggc atcaaccaga tagcttcgtt gcttaagaaa agtaataaaa
      481 ctatcagcgc ccaaaaaaac agtgcgatgc gtcgactggc
//
LOCUS       DADMKF010000417          520 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.422, whole genome shotgun sequence.
ACCESSION   DADMKF010000417 DADMKF010000000
VERSION     DADMKF010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..520
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.422"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            168..503
                     /locus_tag="JNC24_004401"
     CDS             168..503
                     /locus_tag="JNC24_004401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAY9160953.1"
                     /translation="MLSAFVIGAYSAYWLWNNNQPKPFFKDYKTVAEINGCHFNVTED
                     TIDGLKEFDKYKTRILDSGINCKKYPWLYFPLAKSSPGMIVMACNKNYNQHEVADCLT
                     LSYREVNRD"
BASE COUNT          191 a           94 c           94 g          141 t
ORIGIN      
        1 tccataatga cattaacata caaaatcatg taataaactc gtcaacagac gcacatacac
       61 acaatgcccc acctgccata aaagttaatg cgggatacaa agagagcatt ggtggcgcaa
      121 agaatttcaa taacaaaatc ctcaaacata taaaaagtca tctaattatg ttgagcgcat
      181 ttgtcatagg cgcatattct gcatattggc tatggaataa taatcaaccg aagccatttt
      241 tcaaggatta caagactgtt gcggaaatta atggctgcca ttttaatgta acagaagata
      301 caattgatgg gttgaaagag ttcgataaat ataaaacacg gatactggat tcggggatta
      361 actgtaaaaa atatccgtgg ttatacttcc ctcttgcaaa atcctcaccg gggatgattg
      421 ttatggcatg caataaaaac tataaccaac atgaagtggc agactgttta accctgtctt
      481 accgtgaggt taatcgtgat tgattataaa aaaaacttat
//
LOCUS       DADMKF010000418          507 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.424, whole genome shotgun sequence.
ACCESSION   DADMKF010000418 DADMKF010000000
VERSION     DADMKF010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..507
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.424"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(<1..188)
                     /locus_tag="JNC24_004402"
     CDS             complement(<1..188)
                     /locus_tag="JNC24_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY9160954.1"
                     /translation="MTKNTRFSPEVRQRAIRMVLESQDEYDSQWAAICSIAPKIGCTP
                     ETLRVRVRQHERDTGGGDG"
     gene            242..445
                     /locus_tag="JNC24_004403"
                     /pseudo
     CDS             242..445
                     /locus_tag="JNC24_004403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414790.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
BASE COUNT          126 a          135 c          117 g          129 t
ORIGIN      
        1 ccatcaccgc ccccggtatc ccgctcatgc tggcgaaccc ggacacgcag agtctccggc
       61 gtacagccaa tctttggggc aatggaacaa attgccgccc actgtgagtc atattcatcc
      121 tgactttcca gaaccatacg aatcgcccgc tgacggactt cgggggaaaa acgagtattt
      181 ttagtcatcc tgtttacctc tttctcaggg agtttagtct ccaggatttc cggggcggtt
      241 cagacatcta gaatttactg tcagcaccgg cgttaaagtt tacttctgcg atcctcagag
      301 tccttggcag cggggaacaa atgagaatac aaatgggcta attcggcagt actttcctaa
      361 aaagacatgt cttgcccaat atactcaaca tcgagtaggc agcctgacgg ctgcggcttg
      421 tcatggtctg caattaccgt tataaaaaca ggcaatgtca ttgtctttca ggtggttata
      481 tggcccgttc agctaaaccc cgtaaac
//
LOCUS       DADMKF010000419          504 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.425, whole genome shotgun sequence.
ACCESSION   DADMKF010000419 DADMKF010000000
VERSION     DADMKF010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..504
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.425"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            224..>504
                     /locus_tag="JNC24_004404"
     CDS             224..>504
                     /locus_tag="JNC24_004404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY9160955.1"
                     /translation="MKKVFAKSLLVAAMFSVAGSALAVQKDITVTANVDAALDMTQTD
                     NTALPKAVEMQYLPGQGLQSYQLMTKIWSNDVTKDVKMQLVSPAQLVQSL"
BASE COUNT          172 a           96 c          107 g          129 t
ORIGIN      
        1 gcttaaagat tttaacgatc gcttattgta aaaataatag caaagaaaat tgtaagatac
       61 agaccgtaaa taaaaacatc ttccttggtc aggaaagaaa tcttgaaagc atatctggct
      121 acgacaaaat cgttgtcaaa tataataact ggatcaccaa agataatggc gagtttgaac
      181 tggcggtcca ttaattatca ttgtaataaa cgaaggaata gtaatgaaaa aggtatttgc
      241 aaaatctctt ctggtcgcag cgatgttttc tgttgcaggc tccgcattgg cagtacaaaa
      301 ggatattact gtaacggcca acgttgatgc cgctctggat atgacacaga ccgataacac
      361 cgcgttgccg aaagcagtag aaatgcaata tctgccgggg cagggtctgc aatcttacca
      421 gttgatgacc aaaatctggt ctaacgacgt taccaaagat gtaaaaatgc agttagtttc
      481 cccggcgcaa ctggttcaaa gcct
//
LOCUS       DADMKF010000420          489 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.426, whole genome shotgun sequence.
ACCESSION   DADMKF010000420 DADMKF010000000
VERSION     DADMKF010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..489
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.426"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(<1..>489)
                     /gene="ipaH9.8"
                     /locus_tag="JNC24_004405"
     CDS             complement(<1..>489)
                     /gene="ipaH9.8"
                     /locus_tag="JNC24_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH9.8"
                     /protein_id="HAY9160956.1"
                     /translation="TGALLSLGREMFRLEILEDIARDKVRTLHFVDEIEVYLAFQTML
                     AEKLQLSTAVKEMRFYGVSGVTANDLRTAEAMVRSREENEFTDWFSLWGPWHAVLKRT
                     EADRWALAEEQKYEMLENEYPQRVADRLKASGLSGDADAEREAGAQVMRETEQQIYRQ
                     LTD"
BASE COUNT           99 a          153 c          117 g          120 t
ORIGIN      
        1 gtcagtcagc tgacggtaaa tctgctgttc agtctcacgc atcacctgtg caccggcttc
       61 cctctccgca tccgcatcac cgctcagacc tgatgctttc agccggtcag ccaccctctg
      121 agggtactca ttctccagca tctcatattt ctgctcttct gccagcgccc agcggtcagc
      181 ttccgtacgc ttcagtacag catgccatgg tccccagagg gagaaccagt ccgtaaattc
      241 attctcttca cggcttctga ccatggcttc ggcagtgcgg aggtcatttg ctgtcactcc
      301 cgacacgcca tagaaacgca tttccttcac ggcagtggag agctgaagtt tctctgcgag
      361 catggtctgg aaggccaggt agacttctat ctcatccaca aaatggagag ttctgacttt
      421 atcccgggca atgtcctcca gaatttcgag gcggaacatt tccctgccca gggagagcag
      481 agcgccggt
//
LOCUS       DADMKF010000421          488 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.427, whole genome shotgun sequence.
ACCESSION   DADMKF010000421 DADMKF010000000
VERSION     DADMKF010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..488
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.427"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            1..488
                     /locus_tag="JNC24_004406"
                     /pseudo
     CDS             1..488
                     /locus_tag="JNC24_004406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS1-like element IS1A family transposase"
BASE COUNT          116 a          121 c          145 g          106 t
ORIGIN      
        1 actgcacgca ttatgggcgt tggcctcaac acgattttac gtcacttaaa aaactcaggc
       61 cgcagtcggt aacctcgcgc atacagccgg gcagtgacgt catcgtctgc gcggaaatgg
      121 acgaacagtg gggctatgtc ggggctaaat cgcgccagcg ctggctgttt tacgcgtatg
      181 acagtctccg gaagacggtt gttgcgcacg tattcggtga acgcactatg gcgacgctgg
      241 ggcgtcttat gagcctgctg tcaccctttg acgtggtgat atggatgacg gatggctggc
      301 cgctgtatga atcccgcctg aagggaaagc tgcacgtaat cagcaagcga tatacgcagc
      361 gaattgagcg gcataacctg aatctgaggc agcacctggc acggctggga cggaagtcgc
      421 tgtcgttctc aaaatcggtg gagctgcatg acaaagtcat cgggcattat ctgaacataa
      481 aacactat
//
LOCUS       DADMKF010000422          483 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.428, whole genome shotgun sequence.
ACCESSION   DADMKF010000422 DADMKF010000000
VERSION     DADMKF010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..483
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.428"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(<1..>483)
                     /locus_tag="JNC24_004407"
     CDS             complement(<1..>483)
                     /locus_tag="JNC24_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001578320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HAY9160957.1"
                     /translation="SRNGNITRWFYDSSRSGLPCAVEDGTGVRRRITRNRYGQLQAFT
                     DCSGYTTRYEYDRYGQQIAVHREEGISTYSSYNPRGQLVSQRDAQGRETRYEYSAAGD
                     LTAIVAPDGSRSEIQYDAWGKAVSTTQGGLTRSMGYDAAGRITVLTNENGSQSTFRYD
                     "
BASE COUNT           82 a          158 c          134 g          109 t
ORIGIN      
        1 ggtcataccg gaacgtggac tggctgccgt tctcgttggt cagcacagtg atgcgcccgg
       61 cggcgtcata ccccatgctt cgcgtcagac cgccctgcgt ggtgctgacg gcctttcccc
      121 acgcatcata ctgtatctca ctgcggctgc cgtccggggc aacgatagcg gtgaggtctc
      181 ctgcggcgct gtactcataa cgggtttcac gcccctgcgc atccctctga ctgaccagtt
      241 gaccgcgcgg gttataactg ctgtaagtgc tgatgccttc ttcccggtga acggcgattt
      301 gttgaccgta ccggtcatac tcataccgcg tcgtgtaccc cgagcagtcc gtaaacgcct
      361 gaagctgacc gtaccggttt cgcgttattc tccgcctcac cccggttccg tcttccaccg
      421 cacagggcag gccactgcgg gaagaatcat aaaaccagcg cgtaatgtta ccgttgcgcg
      481 agg
//
LOCUS       DADMKF010000423          476 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.429, whole genome shotgun sequence.
ACCESSION   DADMKF010000423 DADMKF010000000
VERSION     DADMKF010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..476
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.429"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            <1..>476
                     /locus_tag="JNC24_004408"
     rRNA            <1..>476
                     /locus_tag="JNC24_004408"
                     /product="16S ribosomal RNA"
BASE COUNT          119 a          115 c          142 g          100 t
ORIGIN      
        1 tgtcgtcagc tcgtgttgtg aaatgttggg ttaagtcccg caacgagcgc aacccttatc
       61 ctttgttgcc agcggtccgg ccgggaactc aaaggagact gccagtgata aactggagga
      121 aggtggggat gacgtcaagt catcatggcc cttacgacca gggctacaca cgtgctacaa
      181 tggcgcatac aaagagaagc gacctcgcga gagcaagcgg acctcataaa gtgcgtcgta
      241 gtccggattg gagtctgcaa ctcgactcca tgaagtcgga atcgctagta atcgtggatc
      301 agaatgccac ggtgaatacg ttcccgggcc ttgtacacac cgcccgtcac accatgggag
      361 tgggttgcaa aagaagtagg tagcttaacc ttcgggaggg cgcttaccac tttgtgattc
      421 atgactgggg tgaagtcgta acaaggtaac cgtaggggaa cctgcggttg gatcac
//
LOCUS       DADMKF010000424          472 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.430, whole genome shotgun sequence.
ACCESSION   DADMKF010000424 DADMKF010000000
VERSION     DADMKF010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..472
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.430"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            <1..111
                     /locus_tag="JNC24_004409"
     CDS             <1..111
                     /locus_tag="JNC24_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAY9160958.1"
                     /translation="PGGDVYLTPMNMTTKPSDGSKAGKQKDNANADETTS"
     gene            89..451
                     /locus_tag="JNC24_004410"
     CDS             89..451
                     /locus_tag="JNC24_004410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HAY9160959.1"
                     /translation="MQTKQRLDVPLSLKSVSDSGEFEGYGSVFGVKDSHDDVVMSGAF
                     AASLRAWSDRKALPALLWQHRMDEPIGVYTEMKEDDVGLYVRGRLLIDDDPLAKRAHA
                     HMKAGSLTGLSIGYVLKD"
BASE COUNT          114 a          112 c          138 g          108 t
ORIGIN      
        1 cccggtggtg atgtctatct cacaccgatg aacatgacca cgaaaccctc cgatggcagt
       61 aaggccggta agcagaagga taacgccaat gcagacgaaa caacgtcttg atgtaccgct
      121 gagtctgaaa tctgtcagtg actccggtga gtttgaaggg tatggctccg tctttggtgt
      181 aaaggacagc cacgatgatg tggtgatgtc cggggcattt gctgcttccc tgcgggcgtg
      241 gagtgacaga aaagcgttac ctgcgctgct ctggcagcac cgcatggatg aacccatcgg
      301 tgtttacacc gaaatgaagg aagacgatgt cgggctttac gtcaggggac ggttgcttat
      361 tgatgatgat cccctcgcaa aacgtgcaca tgcacacatg aaggccggtt cgttaaccgg
      421 cctttctatt gggtacgtcc tgaaagactg aggtagcctg agtttaacgg ac
//
LOCUS       DADMKF010000425          461 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.431, whole genome shotgun sequence.
ACCESSION   DADMKF010000425 DADMKF010000000
VERSION     DADMKF010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..461
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.431"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            2..169
                     /locus_tag="JNC24_004411"
     CDS             2..169
                     /locus_tag="JNC24_004411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HAY9160960.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
BASE COUNT          125 a          109 c           81 g          146 t
ORIGIN      
        1 aatgaacaaa tttattaaag ttgcactggt aggtgcagta ctggctacgt taactgcatg
       61 tactggtcat attgaaaacc gtgataagaa ctgctcttac gactacctgc tgcacccggc
      121 aatttctatt tctaaaatca ttggcggttg cggtcctact gcacagtaag tctctgacag
      181 aaatcggcta acaccggttt aattgcccga cgccctctgg attttccgag ggcgtatttg
      241 tttctatgtc cttcttgccc ccgcccgtaa aaatattttc actttaaatt caatttgata
      301 actacatcca ttagccacag cgcgatcctg tgcgaaattt tgtgatcttc ctccacatta
      361 cataacatca tatgttgtta tattcatcat gcattgtcat gttacctttt aaatgactgc
      421 aaactctccc ctacaacgta ttggacaaga aaaaggtatc g
//
LOCUS       DADMKF010000426          461 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.432, whole genome shotgun sequence.
ACCESSION   DADMKF010000426 DADMKF010000000
VERSION     DADMKF010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..461
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.432"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            <1..>461
                     /locus_tag="JNC24_004412"
     CDS             <1..>461
                     /locus_tag="JNC24_004412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY9160961.1"
                     /translation="LLTSMTDAMRFPGGEMADLYSHRWEIELGYREIKQTMQLSRLTL
                     RSKKPELVEQELWGVLLAYNLVRYQMIKMAEHLKGYWPNQLSFSESCGMVMRMLMTLQ
                     GASPGRIPELMRDLASMGQLVKLPTRRERAFPRVVKERPWKYPTAPKKSQS"
BASE COUNT          134 a           94 c          143 g           90 t
ORIGIN      
        1 tctgctgacg tcgatgacgg acgccatgcg cttccccgga ggagaaatgg cggatctgta
       61 cagtcatcgc tgggaaatcg aactgggata cagggagata aaacagacga tgcaactgag
      121 caggctgacg ctgagaagta aaaagccgga gcttgtggag caagagctgt ggggtgtctt
      181 actggcttat aatctggtga gatatcagat gattaaaatg gcggaacatc tgaaaggtta
      241 ctggccgaat caactgagtt tctcagaatc atgcggaatg gtgatgagaa tgctgatgac
      301 attgcagggc gcttcaccgg gacgtatacc ggagctgatg cgcgatcttg caagtatggg
      361 acaacttgtg aaattaccga cgagaaggga aagggccttc ccgagagtgg taaaggagag
      421 gccctggaaa taccccacag ccccgaaaaa gagccagtca g
//
LOCUS       DADMKF010000427          456 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.433, whole genome shotgun sequence.
ACCESSION   DADMKF010000427 DADMKF010000000
VERSION     DADMKF010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..456
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.433"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            <1..386
                     /locus_tag="JNC24_004413"
     CDS             <1..386
                     /locus_tag="JNC24_004413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HAY9160962.1"
                     /translation="KGQVVELVRLSGRETLRQLEAKTGATRYLMSVLARELVASGDVY
                     NSGYGLFPSEQARKDWQNARKKLSRANLKKPSVVDPDLIWSLPDGEIRRYDRRQNIIC
                     SECRKSEAMQRVLAFYQGNFQKVLL"
BASE COUNT          133 a           96 c          122 g          105 t
ORIGIN      
        1 ttaaagggca ggttgttgaa cttgtacgtc tgagcggtcg cgagacgttg cggcaactgg
       61 aagccaagac aggtgcgaca agatatctga tgagtgttct cgccagagag ctggttgcca
      121 gtggcgatgt atacaactct ggttacgggt tattcccgtc tgaacaggcg cgtaaggact
      181 ggcaaaacgc ccgcaaaaaa ctctcaaggg caaatctgaa gaaaccatct gtggttgatc
      241 ctgaccttat ctggtcatta ccagacggag aaatacgccg ctacgacagg cgtcaaaaca
      301 taatctgtag cgagtgccgg aagagcgaag ctatgcagcg tgtactggct ttctatcagg
      361 gtaattttca gaaggtgctg ttgtgagcca aattaacaat cggaacttcg tgaagagaaa
      421 gcataatcca aaatctgaat aattaagttc agcact
//
LOCUS       DADMKF010000428          452 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.434, whole genome shotgun sequence.
ACCESSION   DADMKF010000428 DADMKF010000000
VERSION     DADMKF010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..452
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.434"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            <1..>452
                     /locus_tag="JNC24_004414"
     CDS             <1..>452
                     /locus_tag="JNC24_004414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HAY9160963.1"
                     /translation="TRAERFRMLDAPALSPLPLEPYEYTEYKAVKVGPDYHVEYARHW
                     YSVPHELVGQRLSLKVGQSVVQLWHKGQCVAQHPRSTHEYKHTTNPLHMPERHRRHGT
                     WTPERLIEQGNRTGPSTGRVVESMLKAKPHPELAYRAVLGLLALQKKY"
BASE COUNT          111 a          137 c          122 g           82 t
ORIGIN      
        1 aacccgcgca gaacgcttcc gcatgctgga tgcaccagca ctctctccgc tgcctctgga
       61 gccttatgaa tacacggaat acaaagccgt gaaggttggc cctgactacc acgttgaata
      121 tgcccgtcac tggtactccg ttccacacga actggtcggt cagcgtctgt cactgaaggt
      181 ggggcagtcg gtggtacagc tctggcacaa ggggcagtgt gtggcgcaac atccgcgcag
      241 cacacatgaa tataaacaca caaccaatcc gcttcacatg ccagaacgcc accggcggca
      301 tgggacatgg acaccggaac gccttatcga gcagggaaac cgtacaggtc cgtccacagg
      361 ccgggtggtt gaatccatgc tgaaagccaa accccatccg gagctggctt accgtgcagt
      421 cctgggtctg ctggcacttc agaaaaaata cg
//
LOCUS       DADMKF010000429          443 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.435, whole genome shotgun sequence.
ACCESSION   DADMKF010000429 DADMKF010000000
VERSION     DADMKF010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.435"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            <1..>443
                     /locus_tag="JNC24_004415"
     CDS             <1..>443
                     /locus_tag="JNC24_004415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HAY9160964.1"
                     /translation="CSDGFEFRCDNGEKLRVTFALDCCDREALHWAVTTGGFNSETVQ
                     DVMLGAVERRFGNELPASPVEWLTDNGSCYRANETRQFARMLGLEPKSTAVRSPESNG
                     IAESFVKTIKRDYISVMPKPDGLTAAKNLAEAFEHYNEWHPHSAL"
BASE COUNT          109 a          106 c          144 g           84 t
ORIGIN      
        1 ggtgctctga cgggttcgag ttccgctgtg ataacggaga aaaactgcga gtcacgttcg
       61 cgctggactg ctgtgaccgt gaggcactgc actgggcggt cactacgggc ggcttcaaca
      121 gtgaaacagt acaggacgtc atgctgggag cggtggaacg ccgcttcggc aacgagcttc
      181 cggcgtctcc agtagagtgg ctgacggata atggttcatg ctaccgggct aatgaaacac
      241 ggcagtttgc ccggatgttg gggcttgaac cgaagagcac ggcggtgcgg agtccggaga
      301 gtaacggcat agcagagagc ttcgtgaaaa cgataaagcg tgactacatc agtgtcatgc
      361 ccaaaccaga cgggttaacg gcagcaaaga accttgcaga ggcgttcgag cattataacg
      421 aatggcatcc gcatagtgca ctg
//
LOCUS       DADMKF010000430          442 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.436, whole genome shotgun sequence.
ACCESSION   DADMKF010000430 DADMKF010000000
VERSION     DADMKF010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..442
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.436"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            <1..>442
                     /locus_tag="JNC24_004416"
     CDS             <1..>442
                     /locus_tag="JNC24_004416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HAY9160965.1"
                     /translation="HRLVHYTRTQYAEPLVESRYLYDPLGRRVAKRVWRRERDLTGWM
                     SLSRKPQVTWYGWDGDRLTTIQNDRTRIQTIYQPGSFTPLIRVETATGELAKTQRRSL
                     ADALQQSGGEDGGSVVFPPVLVQMLDRLESEILADRVSEESRRWL"
BASE COUNT           94 a          127 c          153 g           68 t
ORIGIN      
        1 caccggctgg tgcactacac gcggacacaa tatgcagagc cgctggtcga aagtcgctat
       61 ctttacgacc cgctgggccg cagggtggca aaacgggtat ggcggcgtga acgggacctg
      121 acgggctgga tgtcgctgtc acggaaaccg caagtgacct ggtacggctg ggacggcgac
      181 cggctgacca caatacagaa cgacagaacc cgcatccaga cgatttatca gccggggagc
      241 ttcacgccac tcatcagggt cgaaactgcc accggtgagc tggcgaaaac gcagcgccgc
      301 agcctggcgg atgcccttca gcagtccggc ggcgaagacg gtggcagtgt ggtgttcccg
      361 ccggtgctgg tgcagatgct cgaccggctg gaaagtgaaa tcctggctga ccgggtgagt
      421 gaggaaagcc gccgctggct gg
//
LOCUS       DADMKF010000431          430 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.437, whole genome shotgun sequence.
ACCESSION   DADMKF010000431 DADMKF010000000
VERSION     DADMKF010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..430
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.437"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
BASE COUNT          138 a           76 c           84 g          132 t
ORIGIN      
        1 tcacataaac caccgattga gtgacagtta ctgaaacaac tatttcgaca caaaatctcg
       61 tctattcaac atcatgcaaa tttgtcagtg actgaattta gtgacagaac cctctggaat
      121 tctgccgtgt aaccacttat atgcagacct gccccgttgc tctgggtttt caggctgcga
      181 tgagtgatga gcgatgattt ccatacagat gatatatcga ctggaattga ttgcctgcaa
      241 ttagatttag ttttattgtg tggatcgttt tagttagtgg ggatgaattt ccgataaatt
      301 aaccaatgga aaggagatga tatgaataaa aagataactg gattattaat aattagtctg
      361 gcgtcaattt tcttaattga ttgcacccag tgccattaac atctatctac acaaaaatag
      421 aggaaaacaa
//
LOCUS       DADMKF010000432          420 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.438, whole genome shotgun sequence.
ACCESSION   DADMKF010000432 DADMKF010000000
VERSION     DADMKF010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..420
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.438"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            41..116
                     /locus_tag="JNC24_004417"
     tRNA            41..116
                     /locus_tag="JNC24_004417"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:74..76,aa:Ile,seq:cat)
BASE COUNT          117 a           79 c           98 g          126 t
ORIGIN      
        1 tttattgcgt ggtttataag tattgttatt gcacactcaa ggccctttag ctcagtggtt
       61 agagcaggcg actcataatc gcttggtcgc tggttcaagt ccagcaaggg ccaccaaatc
      121 attatttacg tatcaggaag agttaagggt acaaggggta ctcgatggcg gtctatattt
      181 catgactaac gccatttcaa ggaagaagtg cggtgggtgg tttcgaatga gggctggtgt
      241 tttggtacgt ggcacgccac tgcaatgtat ttatccatca gtactcattc aaaagtacct
      301 ggaaaaaatg cctgctatgc caagcgggca aattgcttac atgtggaatt aagtaattaa
      361 cgataaagcc agagtattat catttattat accccttcat ttaatcaggt taattactca
//
LOCUS       DADMKF010000433          412 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.440, whole genome shotgun sequence.
ACCESSION   DADMKF010000433 DADMKF010000000
VERSION     DADMKF010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..412
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.440"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            1..412
                     /locus_tag="JNC24_004418"
                     /pseudo
     CDS             1..412
                     /locus_tag="JNC24_004418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT           95 a          109 c          124 g           84 t
ORIGIN      
        1 tcagggcgaa tatgactcac aatgggcgac aatttgttcc attgctccaa agattggctg
       61 tacgccggag actctgcgtg tccgggttcg ccagcatgag cgggataccg ggggcggtga
      121 tggagggctc accaccgctg aacgtcagcg tctgaaagag ctggagcgtg aaaatcgtga
      181 actgcgccgc agtaacgata tccttcgcca ggcttccgct tattttgcga aggcggagtt
      241 tgaccgcctc tggaaaaaat gatgccactg ctggataagc tgcgtgagca gtacggggtc
      301 ggaccgctat gcagcgaact gcatattgcc ccgtcaacgt attaccactg tcagcaacag
      361 cgacatcatc cggataaacg cagtgcccgt gcgcagcgcg atgactggct ga
//
LOCUS       DADMKF010000434          408 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.441, whole genome shotgun sequence.
ACCESSION   DADMKF010000434 DADMKF010000000
VERSION     DADMKF010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..408
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.441"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            54..130
                     /locus_tag="JNC24_004419"
     tRNA            54..130
                     /locus_tag="JNC24_004419"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:88..90,aa:Ile,seq:gat)
     gene            173..248
                     /locus_tag="JNC24_004420"
     tRNA            173..248
                     /locus_tag="JNC24_004420"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:206..208,aa:Ala,seq:tgc)
BASE COUNT          115 a           86 c           97 g          110 t
ORIGIN      
        1 ctttgcagtg ctcacacaga ttgtctgatg aaaatgagca gtaaaacctc tacaggcttg
       61 tagctcaggt ggttagagcg cacccctgat aagggtgagg tcggtggttc aagtccactc
      121 aggcctacca aatttgcatc gcaaatttga agaggtttta actacatgtt atggggctat
      181 agctcagctg ggagagcgcc tgctttgcac gcaggaggtc tgcggttcga tcccgcatag
      241 ctccaccatc tctgtagtga ttaaataaaa aatacttcag agtgtacctg caaaggttca
      301 ctgcgaagtt ttgctcttta aaaatctgga tcaagctgaa aattgaaaca ctgaacaatg
      361 aaagttgttc gtgagtctct caaattttcg caacacgatg atgaatcg
//
LOCUS       DADMKF010000435          407 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.442, whole genome shotgun sequence.
ACCESSION   DADMKF010000435 DADMKF010000000
VERSION     DADMKF010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..407
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.442"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            20..127
                     /locus_tag="JNC24_004421"
     CDS             20..127
                     /locus_tag="JNC24_004421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HAY9160966.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
BASE COUNT           98 a           92 c          129 g           88 t
ORIGIN      
        1 ttacaataca ggcggctata tgacgctcgc agaactgggc atggccttct ggcatgattt
       61 agcggctccg gtcattgctg gtattcttgc cagtatgatc gtgaactggc tgaacaagcg
      121 gaagtaacgt gccatgcggg cgtcaggctg ccgtaatggc aatttgcgcc cggatcaggc
      181 cgcaggggtg aaactctgcg gcctttttcg taagaaaaat gcaacggttg caagcggata
      241 atattctgcg gaatgcgcag cacagagtga agacgtgacg gattccggaa cagaaaaaca
      301 gtcgtagaat cagcgtcaag ccggaagggt tccggtgagg cgcaatgttg cgggggcttt
      361 atccctggtg gcattggttg ctggagagag aaaacccccg cacgttg
//
LOCUS       DADMKF010000436          406 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.443, whole genome shotgun sequence.
ACCESSION   DADMKF010000436 DADMKF010000000
VERSION     DADMKF010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..406
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.443"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            4..>406
                     /locus_tag="JNC24_004422"
     CDS             4..>406
                     /locus_tag="JNC24_004422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001439999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9160967.1"
                     /translation="MFSTNLIQSNYGDLNIKSLAFDSFKERLESTMAALTFFISTGQC
                     DCDEIAESNFNRMIAYMSNINYDASKPGAPALSFDTYLQDNVKYRVIINNLYGSEFRI
                     RDINENFVGMDFTCEFRPEKMTNLINIKNRLV"
BASE COUNT          133 a           61 c           82 g          130 t
ORIGIN      
        1 gagatgtttt ctacaaactt aatacagagt aattatggtg atttaaatat taaaagcctg
       61 gctttcgact cttttaaaga aaggcttgag tctaccatgg cggcactcac tttctttata
      121 tcaacgggtc aatgtgactg cgatgaaata gcagagtcga acttcaatcg tatgattgcc
      181 tatatgtcta acattaacta tgatgcctca aagccaggag ctccagcact tagttttgat
      241 acgtatctgc aggacaatgt taaatataga gtgataatta ataatcttta tggcagtgag
      301 tttcggataa gagatattaa tgagaatttt gttgggatgg attttacctg tgaattccga
      361 cccgaaaaaa tgacgaattt gatcaatata aaaaataggt tggtta
//
LOCUS       DADMKF010000437          398 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.444, whole genome shotgun sequence.
ACCESSION   DADMKF010000437 DADMKF010000000
VERSION     DADMKF010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..398
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.444"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(<1..363)
                     /locus_tag="JNC24_004423"
     CDS             complement(<1..363)
                     /locus_tag="JNC24_004423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105657.1"
                     /note="phosphorylates aminoimidazole ribotide (AIR) which
                     forms 4-amino-5-hydroxymethyl-2-methylpyrimidine phosphate
                     (HMP-P); synthesis of HMP-P from AIR is complex and
                     requires the product of thiC gene at least; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoimidazole riboside kinase"
                     /protein_id="HAY9160968.1"
                     /translation="MPESDGRLLPCPGGAPANVAVGIARLGGISGFIGRVGDDPFGAL
                     MQRTLLTEGVDITYLKQDEWHRTSTVLVDLNDQGERSFTFMVRPSADLFLETTDLPCW
                     RHGEWLHLCSIALSAEPSR"
BASE COUNT          105 a          120 c           89 g           84 t
ORIGIN      
        1 acgcgaaggc tcggcagaca acgcaattga acagagatgt aaccattcgc catgtcgcca
       61 gcagggcaag tctgtcgtct ctaaaaaaag atcggcactg gggcggacca taaacgtaaa
      121 tgaacgttcc ccttgatcgt tcagatcgac aagcaccgtg gatgtccggt gccattcatc
      181 ttgcttcaga tacgtgatat cgactccctc agttagcagc gttctttgca ttaacgcccc
      241 aaaaggatca tcaccgaccc gacctataaa tccacttatt ccgcctaatc tggcgattcc
      301 caccgcaacg ttagctggcg cgccgccagg acaaggcagt aggcgcccgt ctgattctgg
      361 caagagatct acgaccgcat cccctaaaac ccatactt
//
LOCUS       DADMKF010000438          397 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.445, whole genome shotgun sequence.
ACCESSION   DADMKF010000438 DADMKF010000000
VERSION     DADMKF010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..397
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.445"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            167..>397
                     /locus_tag="JNC24_004424"
     CDS             167..>397
                     /locus_tag="JNC24_004424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HAY9160969.1"
                     /translation="MNLVTLKTWGKLRYPDNPPSISTLRRWARNGNIYPAPELHGRSY
                     RVVPEAFYINPNKVDTDITHHQPNGRQGRDSPL"
BASE COUNT          119 a           89 c           88 g          101 t
ORIGIN      
        1 aacgttgggt ttttctgctc attattagct ccttctgatg ccattctatt tcaggaagga
       61 gtgtccgtta aactcaggct acctcacata gctgattatt aaaaatcaac cacacccgcc
      121 agtattctgt atatttactg gcggtcatat cgtgagaggt atggcaatga atcttgtgac
      181 actcaaaacg tggggaaaac tcagatatcc ggataaccca ccatcaatat caacgctgag
      241 acgatgggca aggaatggaa acatttatcc tgcacctgaa ctacacggga ggagttacag
      301 ggtggttccg gaggctttct atatcaaccc aaataaggtt gataccgata taacacacca
      361 tcagcctaat gggcgacaag ggagagacag tccgtta
//
LOCUS       DADMKF010000439          397 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.446, whole genome shotgun sequence.
ACCESSION   DADMKF010000439 DADMKF010000000
VERSION     DADMKF010000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..397
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.446"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            <1..>397
                     /locus_tag="JNC24_004425"
     CDS             <1..>397
                     /locus_tag="JNC24_004425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tail tape measure protein"
                     /protein_id="HAY9160970.1"
                     /translation="QSKSLGESLGGLMSDMSHGVDSLTDISYRVRAFFDEVTLAYRSL
                     NDTQKAVADGLANGLLSALKGLAGIVAVRSGIGAVGGIWNLIRAISTLGNVANTAAGR
                     INSRSTGSVEGGKSRLSVADAITRVMIVGM"
BASE COUNT          104 a           64 c          110 g          119 t
ORIGIN      
        1 tcaatctaaa agtcttggtg aatctcttgg tggcttgatg agcgatatgt cgcatggggt
       61 cgattcacta accgatatta gttacagggt acgagcgttc tttgatgagg tgacattagc
      121 ttatcgttca ctaaatgaca ctcaaaaagc tgtagctgat ggtcttgcaa atggcctgtt
      181 aagtgcatta aaggggcttg ctggtatagt cgctgtacgg tctgggattg gtgctgtagg
      241 tggtatctgg aacttaatta gggccatttc cacgcttgga aatgtggcaa atacagcagc
      301 aggaagaatc aattcacgtt ctactggtag tgttgaaggt ggaaaaagta ggctatctgt
      361 agctgatgca attaccagag taatgattgt cggtatg
//
LOCUS       DADMKF010000440          395 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.447, whole genome shotgun sequence.
ACCESSION   DADMKF010000440 DADMKF010000000
VERSION     DADMKF010000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..395
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.447"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(<1..356)
                     /locus_tag="JNC24_004426"
     CDS             complement(<1..356)
                     /locus_tag="JNC24_004426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HAY9160971.1"
                     /translation="MFPDFFMHIGQALDLVSRYDSLRNPLTSLGDYLDPELISRCLAE
                     SGTVTLRKRRLPLEMMVWCIVGMALERKEPLHQIVNRLDIMLPGNRPFVAPSAVIQAR
                     QRLGSEAVRRVFTKTA"
BASE COUNT          103 a           99 c          117 g           76 t
ORIGIN      
        1 tgcgctgttt tcgtgaacac gcggcggaca gcctcacttc ccaggcgctg gcgggcctga
       61 ataacggcac tgggggcaac gaaggggcga ttgcccggca gcatgatgtc caggcgattc
      121 acaatctggt gaagaggttc tttacgctca agcgccatgc caacaataca ccagaccatc
      181 atttcgaggg gaagacggcg cttgcgtagc gttacagtac ctgattcggc aaggcaacga
      241 gagatgagtt cggggtcgag gtaatccccc agagaagtca gtgggttacg cagagaatcg
      301 taacgggata ccagatcaag agcctgtcca atgtgcataa aaaaatccgg aaacaagtga
      361 gcgtttccgg attcttacac agccactgga tcggt
//
LOCUS       DADMKF010000441          394 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.448, whole genome shotgun sequence.
ACCESSION   DADMKF010000441 DADMKF010000000
VERSION     DADMKF010000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..394
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.448"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            63..>394
                     /locus_tag="JNC24_004427"
     CDS             63..>394
                     /locus_tag="JNC24_004427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YhaV family
                     toxin"
                     /protein_id="HAY9160972.1"
                     /translation="MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKA
                     ATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKNKDWSRVKFGAGRYRLFFRYSEK
                     EKVIILGW"
BASE COUNT          120 a           72 c          100 g          102 t
ORIGIN      
        1 ggaaagaaac ttgtcactgg catggacgtc aacattgatg atgagattgg cgacgacgaa
       61 taatggattt tccacaaagg gttaatggtt gggcgctata tgctcatccc tgttttcagg
      121 aaacctacga cgctttagtt gccgaagtcg agacattaaa gggaaaagat cctgaaaatt
      181 atcagagaaa agccgccaca aagttattgg cggtagtcca taaagtgatt gaggagcata
      241 tcacggtcaa tccatcatca ccggcattcc gtcatggcaa gtcgttaggc tctgggaaaa
      301 ataaagactg gtcacgggta aaatttggtg caggtcgtta tcgtctcttc tttcgttata
      361 gtgaaaaaga gaaagtcatc attctgggat ggat
//
LOCUS       DADMKF010000442          386 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.449, whole genome shotgun sequence.
ACCESSION   DADMKF010000442 DADMKF010000000
VERSION     DADMKF010000442.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..386
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.449"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            <1..>386
                     /locus_tag="JNC24_004428"
     CDS             <1..>386
                     /locus_tag="JNC24_004428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9160973.1"
                     /translation="LYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAYFGLPEALMNR
                     FVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPARAAWIQFLIV
                     IPLMIIPMLGSNTVQDLMNTIINMTA"
BASE COUNT           81 a           98 c          102 g          105 t
ORIGIN      
        1 actgtattcc gtctcctcgg tgctgattaa cgtcttcgtc agcagcaaag agttgaaatt
       61 taccggcgga tcggtgcagg tattccacgg catggcggcg tattttggtc taccggaagc
      121 gttgatgaat cgctttgtcg gtctggtgtc ctttaccgcg atgttcggtt ccctgctgat
      181 gtggaccgca acgccggtga aaattttctt ctccgaaatc ccggaaggca tctttggtaa
      241 gaaaaccgtc gaactgaacg aaaacggcgt tccggcgcgc gcagcgtgga tccagttcct
      301 gatcgtcatc ccgctgatga ttatcccgat gctcggttcc aataccgtgc aggatctgat
      361 gaatactatt attaatatga ccgccg
//
LOCUS       DADMKF010000443          374 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.denovo.450, whole genome shotgun sequence.
ACCESSION   DADMKF010000443 DADMKF010000000
VERSION     DADMKF010000443.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..374
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.denovo.450"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            <1..>374
                     /locus_tag="JNC24_004429"
     CDS             <1..>374
                     /locus_tag="JNC24_004429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HAY9160974.1"
                     /translation="SRLTMKALEMAWETRGKPGGVMFHSDQGSHYTSRQFRQLLWRYQ
                     IRQSMSRRGNCWDNSPMERFFRSLKNEWMPVVGYVSFSEAAHAITDYIVGYYSALRPH
                     EYNGGLPPNESENRYWKNSNSVA"
BASE COUNT          106 a           87 c          111 g           70 t
ORIGIN      
        1 agcaggctca ccatgaaagc gctggaaatg gcatgggaaa cccgtggtaa gcccggcggg
       61 gtgatgttcc acagcgatca gggcagtcat tatacgagca ggcagttccg gcagttattg
      121 tggcgatacc agatcagaca gagtatgagc cggcgcggaa actgctggga taacagccca
      181 atggaacgct tcttcaggag tctgaagaac gaatggatgc cggtggtggg ttacgtaagc
      241 ttcagcgagg cagctcacgc cataacggac tatatcgttg gatattacag cgcactaaga
      301 ccgcacgaat ataacggtgg gttaccccca aacgaatcgg aaaatcgata ctggaaaaac
      361 tctaactcgg tggc
//
LOCUS       DADMKF010000444        32542 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.guided.031, whole genome shotgun sequence.
ACCESSION   DADMKF010000444 DADMKF010000000
VERSION     DADMKF010000444.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32542
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.guided.031"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(10..987)
                     /gene="intI2"
                     /locus_tag="JNC24_004430"
                     /pseudo
     CDS             complement(10..987)
                     /gene="intI2"
                     /locus_tag="JNC24_004430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_023994230.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class 2 integron integrase IntI2"
     gene            1319..1792
                     /locus_tag="JNC24_004431"
     CDS             1319..1792
                     /locus_tag="JNC24_004431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HAY9160975.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            1887..2411
                     /gene="sat2"
                     /locus_tag="JNC24_004432"
     CDS             1887..2411
                     /gene="sat2"
                     /locus_tag="JNC24_004432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat2"
                     /protein_id="HAY9160976.1"
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSP
                     YRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAH
                     SLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNET
                     AMYWYWFSGAQDDA"
     gene            2469..3257
                     /gene="aadA1"
                     /locus_tag="JNC24_004433"
     CDS             2469..3257
                     /gene="aadA1"
                     /locus_tag="JNC24_004433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HAY9160977.1"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
     gene            3333..3830
                     /locus_tag="JNC24_004434"
     CDS             3333..3830
                     /locus_tag="JNC24_004434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9160978.1"
                     /translation="MRDLLKVVLSLVLAMASGQALADLPIVLVDEARLPYDYSPSNYD
                     ISPSNYDNSISNYDNSPSNYDNSESNYDNSSSNYDNSRNGNRRLIYSANGSRTFAGYY
                     VIANNGTTNFFSTSGKRMFYTPKGGRGVYGGKDGSFCGALVVINGQFSLALTDNGLKI
                     MYLSN"
     gene            3891..4262
                     /locus_tag="JNC24_004435"
     CDS             3891..4262
                     /locus_tag="JNC24_004435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9160979.1"
                     /translation="MSYEPPRDGYTNGYIVDVMYDNRPSKYPAVFRTYVRIPDYVELD
                     NAEELADAIIREVELKEAWLTRVLGNSADPDASVGKWDRRLAQCGGIDEMSSGVQATY
                     FSPNGQLVGLVAYIDAEWMDE"
     gene            4672..5049
                     /locus_tag="JNC24_004436"
     CDS             4672..5049
                     /locus_tag="JNC24_004436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9160980.1"
                     /translation="MSAAKALAYALCDKLESKKVDEVVRNLVSSGWNIREVAWEASSF
                     DHEMPYFDTGRDFDIGFPDTLSSKIEFLTANGSIVQARARLHFKQALIFAKASKHVAA
                     LKNSLDYYYGSSVIPPQNHGGYL"
     gene            5072..5224
                     /locus_tag="JNC24_004437"
                     /pseudo
     CDS             5072..5224
                     /locus_tag="JNC24_004437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011069623.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(5280..6896)
                     /locus_tag="JNC24_004438"
     CDS             complement(5280..6896)
                     /locus_tag="JNC24_004438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000251878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator"
                     /protein_id="HAY9160981.1"
                     /translation="MVRLATFNDNVQVVHIGHLFRNSGHKEWRIFVWFNPMQERKWTR
                     FTHLPLLSRAKVVNSTTKQINKADRVIEFEASDLQRAKIIDFPNLSSFASVRNKDGAQ
                     SSFIYEAETPYSKTRYHIPQLELARSLFLINSYFCRSCLSSTALQQEFDVQYEVERDH
                     LEIRILPSSSFPKGALEQSAVVQLLVWLFSDQDVMDSYESIFRHYQQNREIKNGVESW
                     CFSFDPPPMQGWKLHVKGRSSNEDKDYLVEEIVGLEINAMLPSTTAISHASFQEKEAG
                     DGSTQHIAVSTESVVDDEHLQLDDEETANIDTDTRVIEAEPTWISFSRPSRIEKSRRA
                     RKSSQTILEKEEATTSENSNLVSTDEPHLGGVLAAADVGGKQDATNYNSIFANRFAAF
                     DELLSILKTKFACRVLFEETLVLPKVGRSRLHLCKDGSPRVIKAVGVQRNGSEFVLLE
                     VDASDGVKMLSTKVLSGVDSETWRNDFEKIRRGVVKSSLNWPNSLFDQLYGQDGHRGV
                     NHPKGLGELQVSREDMEGWAERVVREQFTH"
     gene            complement(6897..8423)
                     /locus_tag="JNC24_004439"
     CDS             complement(6897..8423)
                     /locus_tag="JNC24_004439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator"
                     /protein_id="HAY9160982.1"
                     /translation="MRNFPVPYSNELIYSTIARAGVYQGIVSPKQLLDEVYGNRKVVA
                     TLGLPSHLGVIARHLHQTGRYAVQQLIYEHTLFPLYAPFVGKERRDEAIRLMEYQAQG
                     AVHLMLGVAASRVKSDNRFRYCPDCVALQLNRYGEAFWQRDWYLPALPYCPKHGALVF
                     FDRAVDDHRHQFWALGHTELLSDYPKDSLSQLTALAAYIAPLLDAPRAQELSPSLEQW
                     TLFYQRLAQDLGLTKSKHIRHDLVAERVRQTFSDEALEKLDLKLAENKDTCWLKSIFR
                     KHRKAFSYLQHSIVWQALLPKLTVIEALQQASALTEHSITTRPVSQSVQPNSEDLSVK
                     HKDWQQLVHKYQGIKAARQSLEGGVLYAWLYRHDRDWLVHWNQQHQQERLAPAPRVDW
                     NQRDRIAVRQLLRIIKRLDSSLDHPRATSSWLLKQTPNGTSLAKNLQKLPLVALCLKR
                     YSESVEDYQIRRISQAFIKLKQEDVELRRWRLLRSATLSKERITEEAQRFLEMVYGEE
                     "
     gene            complement(8426..10093)
                     /locus_tag="JNC24_004440"
     CDS             complement(8426..10093)
                     /locus_tag="JNC24_004440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAY9160983.1"
                     /translation="MSATRIQAVYRDTGVEAYRDNPFIEALPPLQESVNSAASLKSSL
                     QLTSSDLQKSRVIRAHTICRIPDDYFQPLGTHLLLSERISVMIRGGYVGRNPKTGDLQ
                     KHLQNGYERVQTGELETFRFEEARSTAQSLLLIGCSGSGKTTSLHRILATYPQVIYHR
                     ELNVEQVVYLKIDCSHNGSLKEICLNFFRALDRALGSNYERRYGLKRHGIETMLALMS
                     QIANAHALGLLVIDEIQHLSRSRSGGSQEMLNFFVTMVNIIGVPVMLIGTPKAREIFE
                     ADLRSARRGAGFGAIFWDPIQQTQRGKPNQEWIAFTDNLWQLQLLQRKDALLSDEVRD
                     VWYELSQGVMDIVVKLFVLAQLRALALGNERITAGLLRQVYQDELKPVHPMLEALRSG
                     IPERIARYSDLVVPEIDKRLIQLQLDIAAIQEQTPEEKALQELDTEDQRHLYLMLKED
                     YDSSLLIPTIKKAFSQNPTMTRQKLLPLVLQWLMEGETVVSELEKPSKSKKVSAIKVV
                     KPSDWDSLPDTDLRYIYSQRQPEKTMHERLKGKGVIVDMASLFKQAG"
     gene            complement(10090..12198)
                     /locus_tag="JNC24_004441"
     CDS             complement(10090..12198)
                     /locus_tag="JNC24_004441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000267723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HAY9160984.1"
                     /translation="MWQINEVVLFDNDPYRILAIEDGQVVWMQISADKGVPQARAELL
                     LMQYLDEGRLVRTDDPYVHLDLEEPSVDSVSFQKREEDYRKILPIINSKDRFDPKVRS
                     ELVEHVVQEHKVTKATVYKLLRRYWQRGQTPNALIPDYKNSGAPGERRSATGTAKIGR
                     AREYGKGEGTKVTPEIERLFRLTIEKHLLNQKGTKTTVAYRRFVDLFAQYFPRIPQED
                     YPTLRQFRYFYDREYPKAQRLKSRVKAGVYKKDVRPLSSTATSQALGPGSRYEIDATI
                     ADIYLVDHHDRQKIIGRPTLYIVIDVFSRMITGFYIGFENPSYVVAMQAFVNACSDKT
                     AICAQHDIEISSSDWPCVGLPDVLLADRGELMSHQVEALVSSFNVRVESAPPRRGDAK
                     GIVESTFRTLQAEFKSFAPGIVEGSRIKSHGETDYRLDASLSVFEFTQIILRTILFRN
                     NHLVMDKYDRDADFPTDLPSIPVQLWQWGMQHRTGSLRAVEQEQLRVALLPRRKVSIS
                     SFGVNLWGLYYSGSEILREGWLQRSTDIARPQHLEAAYDPVLVDTIYLFPQVGSRVFW
                     RCNLTERSRQFKGLSFWEVWDIQAQEKHNKANAKQDELTKRRELEAFIQQTIQKANKL
                     TPSTTEPKSTRIKQIKTNKKEAVTSERKKRAEHLKPSSSGDEAKVIPFNAVEADDQED
                     YSLPTYVPELFQDPPEKDES"
     gene            complement(12185..13006)
                     /locus_tag="JNC24_004442"
     CDS             complement(12185..13006)
                     /locus_tag="JNC24_004442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heteromeric transposase endonuclease subunit
                     TnsA"
                     /protein_id="HAY9160985.1"
                     /translation="MAKANSSFSEVQIARRIKEGRGQGHGKDYIPWLTVQEVPSSGRS
                     HRIYSHKTGRVHHLLSDLELAVFLSLEWESSVLDIREQFPLLPSDTRQIAIDSGIKHP
                     VIRGVDQVMSTDFLVDCKDGPFEQFAIQVKPAAALQDERTLEKLELERRYWQQKQIPW
                     FIFTDKEINPVVKENIEWLYSVKTEEVSAELLAQLSPLAHILQEKGDENIINVCKQVD
                     IAYDLELGKTLSEIRALTANGFIKFNIYKSFRANKCADLCISQVVNMEELRYVAN"
     gene            complement(13165..14994)
                     /gene="glmS"
                     /locus_tag="JNC24_004443"
     CDS             complement(13165..14994)
                     /gene="glmS"
                     /locus_tag="JNC24_004443"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HAY9160986.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLDGGTGIAHTRWATHGEPSEANAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            complement(15156..16526)
                     /gene="glmU"
                     /locus_tag="JNC24_004444"
     CDS             complement(15156..16526)
                     /gene="glmU"
                     /locus_tag="JNC24_004444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HAY9160987.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGSKVGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            complement(16877..17296)
                     /gene="atpC"
                     /locus_tag="JNC24_004445"
     CDS             complement(16877..17296)
                     /gene="atpC"
                     /locus_tag="JNC24_004445"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HAY9160988.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            complement(17317..18699)
                     /gene="atpD"
                     /locus_tag="JNC24_004446"
     CDS             complement(17317..18699)
                     /gene="atpD"
                     /locus_tag="JNC24_004446"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HAY9160989.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            complement(18726..19589)
                     /gene="atpG"
                     /locus_tag="JNC24_004447"
     CDS             complement(18726..19589)
                     /gene="atpG"
                     /locus_tag="JNC24_004447"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HAY9160990.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKSVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            complement(19640..21181)
                     /gene="atpA"
                     /locus_tag="JNC24_004448"
     CDS             complement(19640..21181)
                     /gene="atpA"
                     /locus_tag="JNC24_004448"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HAY9160991.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            complement(21194..21727)
                     /gene="atpH"
                     /locus_tag="JNC24_004449"
     CDS             complement(21194..21727)
                     /gene="atpH"
                     /locus_tag="JNC24_004449"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HAY9160992.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            complement(21742..22212)
                     /gene="atpF"
                     /locus_tag="JNC24_004450"
     CDS             complement(21742..22212)
                     /gene="atpF"
                     /locus_tag="JNC24_004450"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HAY9160993.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            complement(22274..22513)
                     /gene="atpE"
                     /locus_tag="JNC24_004451"
     CDS             complement(22274..22513)
                     /gene="atpE"
                     /locus_tag="JNC24_004451"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HAY9160994.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            complement(22560..23375)
                     /gene="atpB"
                     /locus_tag="JNC24_004452"
     CDS             complement(22560..23375)
                     /gene="atpB"
                     /locus_tag="JNC24_004452"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HAY9160995.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYSIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            complement(23384..23764)
                     /gene="atpI"
                     /locus_tag="JNC24_004453"
     CDS             complement(23384..23764)
                     /gene="atpI"
                     /locus_tag="JNC24_004453"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HAY9160996.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAYTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            complement(24381..25004)
                     /gene="rsmG"
                     /locus_tag="JNC24_004454"
     CDS             complement(24381..25004)
                     /gene="rsmG"
                     /locus_tag="JNC24_004454"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HAY9160997.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            complement(25068..26957)
                     /gene="mnmG"
                     /locus_tag="JNC24_004455"
     CDS             complement(25068..26957)
                     /gene="mnmG"
                     /locus_tag="JNC24_004455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HAY9160998.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     LYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASQHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLVGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            complement(27336..27779)
                     /gene="mioC"
                     /locus_tag="JNC24_004456"
     CDS             complement(27336..27779)
                     /gene="mioC"
                     /locus_tag="JNC24_004456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HAY9160999.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     SASGIWLIISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            complement(27869..28327)
                     /gene="asnC"
                     /locus_tag="JNC24_004457"
     CDS             complement(27869..28327)
                     /gene="asnC"
                     /locus_tag="JNC24_004457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HAY9161000.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLAEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            28479..29471
                     /gene="asnA"
                     /locus_tag="JNC24_004458"
     CDS             28479..29471
                     /gene="asnA"
                     /locus_tag="JNC24_004458"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HAY9161001.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVSEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRENVPSLL"
     gene            complement(29476..30927)
                     /gene="viaA"
                     /locus_tag="JNC24_004459"
     CDS             complement(29476..30927)
                     /gene="viaA"
                     /locus_tag="JNC24_004459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HAY9161002.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQLIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVIERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(30921..32417)
                     /gene="ravA"
                     /locus_tag="JNC24_004460"
     CDS             complement(30921..32417)
                     /gene="ravA"
                     /locus_tag="JNC24_004460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HAY9161003.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRLQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTVIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSAQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEASLQDVGAQIHQAQQC"
BASE COUNT         8249 a         8264 c         7617 g         8412 t
ORIGIN      
        1 tatctacttt tactgattga taagtagcat cagtccatcc gcaggactgg tggtgccggc
       61 aaaatgctga cccaacacat gcgtatagat ttgcgtggtc ttaacatcgt tatgccctaa
      121 gagttcttgc acagtgcgaa tatcacgccc cgcttgtaat agatgcgtag caaacgagtg
      181 acgaaatgta tgacaagtga cacgcttgct aacgatgcct gctttttgta cggctgcctt
      241 caatgccttt cgcgcaacgg agtcatgcag atgatggcgg cataatttgc cgttatacgg
      301 gtggttgcag agcgtgctgg agggaaagac aaacatccac gccgcttgtc gataagcaga
      361 agggtatttg tgatctaaag caaaaggcag cgatggccct acgccttgta agttgtcgtc
      421 ttgctgaata agccgcgctt gctcaatgag ttattttatt gctgggatta ggcgcgtggg
      481 cagtaggctg tttctgcttt tcccaccctt accgtcatgc acagtgatgc agccattatc
      541 aaaatcaaaa tctttaaccc gcaaacgcaa gcattcatta atgcgcaaac ctgcaccata
      601 cagcagcgta aaaataactt ggttgcgagt atccataacc tgcaaaatgc gttgcacttc
      661 atttgcagag ataacagagg gtagccgtct aggcttgctt gcagggatat aatcaatatc
      721 gcccaacggc tgttgtaaaa acctgttgta caaaaaagct agggcattta aagcgatttt
      781 ctgcgtgttt atggctacat gtctgctgtt tgctaagctg gataaaaaca gcctgacctc
      841 ttcactgccc atggtctgag gatgacgttt tttgtgaaac agaataaaac gcttaatcca
      901 gtgcaggtaa gttttttcag ttttcagcgc ataacctttt tgtcgcatat ccgtgcgtat
      961 agaatttaaa aatggactgt tagacataaa acgctccttg tcttgcaact gtctgcctat
     1021 acagcctatt ctagctggga tttaaaaaag tgcctgtttt ttacgcctag agatgcttgt
     1081 ttaccggtag agttttaatt taatgctaaa taaattaaaa tgttatgagt tctttgggtg
     1141 agataatgtg catcgtgcaa gcaggataga cggcatgcac gatttgtaat aacagagtgt
     1201 cttgtatttt taaagaaagt ctatttaata caagtgatta tattaattaa cggtaagcat
     1261 cagcgggtga caaaacgagc atgcttacta ataaaatgtt aacctctgag gaagaattgt
     1321 gaaactatca ctaatggtag ctatatcgaa gaatggagtt atcgggaatg gccctgatat
     1381 tccatggagt gccaaaggtg aacagctcct gtttaaagct attacctata accaatggct
     1441 gttggttgga cgcaagactt ttgaatcaat gggagcatta cccaaccgaa agtatgcggt
     1501 cgtaacacgt tcaagtttta catctgacaa tgagaacgta ttgatctttc catcaattaa
     1561 agatgcttta accaacctaa agaaaataac ggatcatgtc attgtttcag gtggtgggga
     1621 gatatacaaa agcctgatcg atcaagtaga tacactacat atatctacaa tagacatcga
     1681 gccggaaggt gatgtttact ttcctgaaat ccccagcaat tttaggccag tttttaccca
     1741 agacttcgcc tctaacataa attatagtta ccaaatctgg caaaagggtt aacaagtggc
     1801 agcaacggat tcgcaaacct gtcacgcctt ttgtaccaaa agccgcgcca ggtttgcgat
     1861 ccgctgtgcc aggcgttagg cgtcatatga agatttcggt gatccctgag caggtggcgg
     1921 aaacattgga tgctgagaac catttcattg ttcgtgaagt gttcgatgtg cacctatccg
     1981 accaaggctt tgaactatct accagaagtg tgagccccta ccggaaggat tacatctcgg
     2041 atgatgactc tgatgaagac tctgcttgct atggcgcatt catcgaccaa gagcttgtcg
     2101 ggaagattga actcaactca acatggaacg atctagcctc tatcgaacac attgttgtgt
     2161 cgcacacgca ccgaggcaaa ggagtcgcgc acagtctcat cgaatttgcg aaaaagtggg
     2221 cactaagcag acagctcctt ggcatacgat tagagacaca aacgaacaat gtacctgcct
     2281 gcaatttgta cgcaaaatgt ggctttactc tcggcggcat tgacctgttc acgtataaaa
     2341 ctagacctca agtctcgaac gaaacagcga tgtactggta ctggttctcg ggagcacagg
     2401 atgacgccta acaattcatt caagccgaca ccgcttcgcg gcgcggctta attcaggagt
     2461 taaacatcat gagggaagcg gtgatcgccg aagtatcgac tcaactatca gaggtagttg
     2521 gcgtcatcga gcgccatctc gaaccgacgt tgctggccgt acatttgtac ggctccgcag
     2581 tggatggcgg cctgaagcca cacagtgata ttgatttgct ggttacggtg accgtaaggc
     2641 ttgatgaaac aacgcggcga gctttgatca acgacctttt ggaaacttcg gcttcccctg
     2701 gagagagcga gattctccgc gctgtagaag tcaccattgt tgtgcacgac gacatcattc
     2761 cgtggcgtta tccagctaag cgcgaactgc aatttggaga atggcagcgc aatgacattc
     2821 ttgcaggtat cttcgagcca gccacgatcg acattgatct ggctatcttg ctgacaaaag
     2881 caagagaaca tagcgttgcc ttggtaggtc cagcggcgga ggaactcttt gatccggttc
     2941 ctgaacagga tctatttgag gcgctaaatg aaaccttaac gctatggaac tcgccgcccg
     3001 actgggctgg cgatgagcga aatgtagtgc ttacgttgtc ccgcatttgg tacagcgcag
     3061 taaccggcaa aatcgcgccg aaggatgtcg ctgccgactg ggcaatggag cgcctgccgg
     3121 cccagtatca gcccgtcata cttgaagcta ggcaggctta tcttggacaa gaagatcgct
     3181 tggcctcgcg cgcagatcag ttggaagaat ttgttcacta cgtgaaaggc gagatcacca
     3241 aggtagtcgg caaataatgt ctaacaattc gttcaagccg acgccgcttc gcggcgcggc
     3301 ttaactcaag cgttagagag ctggggaaga ctatgcgcga tctgttgaag gtggttctaa
     3361 gcctcgtact tgcgatggca tcggggcagg cacttgctga cctgccaatt gttttagtgg
     3421 atgaagctcg tcttccctat gactactccc catccaacta cgacatttct ccaagcaact
     3481 acgacaactc cataagcaat tacgacaata gtccatcaaa ttacgacaac tctgagagca
     3541 actacgataa tagttcatcc aattacgaca atagtcgcaa cggaaatcgt aggcttatat
     3601 atagcgcaaa tgggtctcgc actttcgccg gctactacgt cattgccaac aatgggacaa
     3661 cgaacttctt ttccacatct ggcaaaagga tgttctacac cccaaaaggg gggcgcggcg
     3721 tctatggcgg caaagatggg agcttctgcg gggcattggt cgtcataaat ggccaatttt
     3781 cgcttgccct gacagataac ggcctgaaga tcatgtatct aagcaactag cctgctctct
     3841 aataaaatgt taggcctcaa catctagtcg caagctgagg ggaaccacta gtgtcatacg
     3901 aacctccaag agacggttac acaaacgggt acattgttga tgtcatgtat gacaatcgcc
     3961 caagtaagta tccagctgtg ttcagaacgt acgtccgaat tccagactat gtcgaactag
     4021 ataacgcgga ggagctggcc gacgcaatca ttcgagaggt tgagttaaag gaggcatggc
     4081 tcacaagggt cttggggaac tcagccgatc ctgacgcttc tgtgggcaag tgggaccggc
     4141 gcctagcgca gtgcggtggt attgatgaga tgtcaagtgg ggtgcaggca acatacttca
     4201 gtccaaacgg tcagctggtc ggcttagtgg catacattga tgccgaatgg atggatgaat
     4261 agctctgacg ttacgattca ggtgagacaa ccagatgggc attgcgtaca agctcagttg
     4321 ccaccgatgt ggctacgagt ctgatcttct ttacctaggc caaggtatgg cgatgcttcc
     4381 tgagcaaata gtcggaacgt gcacatgctg cgacaatcta acgacgattt ctataaatga
     4441 aagctacgga gtatgctcac tttgtggggc taaggatcgt gtgatattta caagctatca
     4501 gcatgaggca aggccaagaa atccctgcta tctagagaga agtttcaaat atcaatgccc
     4561 aagatgccat gccttcagta tggagcctcc ggatctaccg gagatgcttt gggactaatg
     4621 ctccgctttg cctcgggtat ccgttcgacc tatgtttaag aggaaacgca cgtgtctgcc
     4681 gccaaagctc ttgcctatgc actttgtgac aagttggaaa gcaaaaaagt tgatgaagtc
     4741 gtacgaaatc ttgtttctag cggctggaat attagggaag tggcctggga agcttcgtcc
     4801 tttgatcatg aaatgcccta ctttgacacg gggcgggact tcgatattgg atttcctgac
     4861 acgctgtcat ccaagattga gtttttgacc gcgaatggat caatcgtcca agcacgtgct
     4921 cgcctgcact tcaagcaggc tctaattttt gccaaggcgt ccaaacatgt cgccgcgctc
     4981 aagaactcat tggattacta ctatggatcg tctgtaatcc cccctcaaaa ccacggaggc
     5041 tatttgtaga aaattccatt aatatgaact caaggtgttc cctacggacg aatcagcgat
     5101 gaaggtggtg tacctggcta tccaggcggc gaccaagaaa tggacgatgc cgatccgcaa
     5161 ctggaagccg gccatgaacc gttttatgat cgaatttggt gaccgtttaa atggccacct
     5221 ttaactgacc ggcaaaagca cttacacaaa agggtttaca ggctctttca gtcattcctt
     5281 taatgcgtaa attgctctct aaccactctc tcagcccacc cttccatatc ctctctcgat
     5341 acttgcagct cccccaaccc ctttggatga ttcacccctc tatgcccgtc ttgtccatat
     5401 aattgatcaa acaaactatt tggccaattc aatgagctct tcaccactcc acgccgtatc
     5461 ttttcaaaat cattccgcca tgtttcgcta tcaacgccac tcaacacttt ggtagaaagc
     5521 attttcaccc catccgatgc atccacctct agcaatacaa attcactgcc attacgttgc
     5581 accccaacgg ctttaatcac tcttggtgag ccatctttac acagatgtaa tcggctacgc
     5641 ccaacttttg gcaaaaccaa ggtttcttca aaaagcaccc gacatgcaaa tttagttttt
     5701 agaattgaaa gtagctcatc aaaagcagca aatcgattag caaaaataga gttgtaattg
     5761 gttgcatcct gcttcccacc cacatctgcc gctgctagga caccacctaa gtgtggctca
     5821 tcagtactaa ccaaattact attttcactt gttgttgctt cttctttttc taaaatagtt
     5881 tggctacttt ttcttgccct gcgagatttt tcaattcgac taggtctact aaaacttatc
     5941 catgtcggct cagcctctat gactcgtgtg tctgtgtcta tattggctgt ttcctcatcg
     6001 tccaactgta gatgctcatc atcaacaact gactctgttg aaaccgctat gtgctgcgta
     6061 ctaccatcac ctgcctcctt ttcctgaaaa gaggcatggc taatagctgt tgtgctagga
     6121 agcatagcgt tgatttctaa acctactatt tcctcaacta aataatcctt atcctcgtta
     6181 gaagaacgtc cttttacatg taatttccaa ccctgcatgg gcggagggtc aaagctaaag
     6241 caccagcttt caacgccgtt cttaatttct ctattttgtt gatagtgcct aaaaatactt
     6301 tcatacgaat ccataacatc ttgatccgaa aacaaccaaa ccaaaagctg cactacggcc
     6361 gactgctcta acgccccttt aggaaacgat gaactgggta agatccttat ctctaaatga
     6421 tctcgctcaa cctcatactg aacgtcgaac tcttgctgta aagcggtact gctcaaacag
     6481 cttcgacaga aataggagtt aattaaaaat aatgaccgag ctagctctaa ctgtgggatg
     6541 tgataacgag tcttgctata tggtgtttca gcttcgtaaa taaatgaact ctgcgctcca
     6601 tccttgttgc gtacggaagc aaaggacgag agattaggaa aatcgattat tttggctcgt
     6661 tgaagatccg atgcttcaaa ctcaatcaca cgatccgcct tatttatttg ctttgttgta
     6721 ctgttaacca ccttagctcg acttaataaa ggcaaatgag taaatcgagt ccatttccgt
     6781 tcttgcattg gattaaacca aacaaaaata cgccactcct tatgacccga gttacggaat
     6841 aaatgaccaa tatgtacaac ctgcacattg tcattaaatg tagctagcct aaccactcac
     6901 tcttccccat aaaccatttc caagaatctt tgtgcttcct cagttatccg ctctttagat
     6961 aacgttgcac ttcttaataa tcgccagcgc ctaagctcaa catcttcctg tttaagctta
     7021 ataaaagctt ggctaatccg tctaatttga taatcttcca cactctctga gtaacgcttt
     7081 aagcaaagcg ctaccaaagg cagtttctgt agattttttg caagagaggt tccgttagga
     7141 gtttgcttta acagccagct cgatgtcgct cttgggtgat caaggctact atctagacgc
     7201 tttatgattc ttaatagttg tcgtacagca attcgatctc tttggttcca atcaactcta
     7261 ggggcgggtg ccagacgctc ttgttgatgc tgttgattcc agtgaactag ccaatccctg
     7321 tcatgtcggt aaagccaagc gtatagcacc ccaccctcta aagactgtct tgccgcctta
     7381 attccttggt atttatgcac tagttgctgc cagtctttat gcttaacaga taaatcttca
     7441 gagttaggtt gcacagactg gctaacaggt cttgtcgtta tagagtgctc agtaagagca
     7501 cttgcctgct gtagcgcttc tataaccgtt agttttggca ataaggcttg ccacacaata
     7561 ctatgctgta aataactaaa ggcttttcta tgcttacgga atatactttt cagccaacac
     7621 gtgtccttgt tctctgccaa ctttaaatcc agtttctcta gtgcctcatc actaaaagtc
     7681 tgcctcactc tctccgccac caagtcatga cgaatgtgct tgcttttggt tagccctaga
     7741 tcctgcgcta agcgctgata aaacagcgtc cactgctcaa ggcttgggga aagctcttgc
     7801 gctcgtggag catctaacag aggggctata taagcagcta gtgctgttaa ttgagatagg
     7861 gagtctttgg ggtagtctga aagcagctca gtatgaccca aagcccaaaa ttgatgtcgg
     7921 tgatcatcta cagctctatc aaagaagact aaagcaccgt gttttggaca atatggcaaa
     7981 gcgggcaaat accaatctcg ttgccaaaag gcttccccat acctatttag ctgaagagca
     8041 acgcaatcag ggcagtatct aaagcggtta tcgctcttaa ctctagaagc agcgactcct
     8101 agcattaaat gcaccgcacc ttgcgcttgg tactccatta accgaatagc ttcgtctcgg
     8161 cgctccttgc ctacaaacgg agcatataaa gggaataagg tatgctcata aataagctgc
     8221 tgaacagcgt aacgtcctgt ttgatgtaga tgtcttgcta tcacacctaa atgcgagggc
     8281 agacctaagg tagcgaccac cttgcggttg ccatacacct catccaacag ctgcttagga
     8341 ctaacaatcc cttgataaac gcctgcccgt gcaatagtgc tataaatcag ctcattcgag
     8401 tacggaacag gaaagtttct catggctaac ctgcttgttt aaataagctc gccatatcca
     8461 ctattacccc tttccctttt aaccgttcat gcatggtttt ttcaggttgg cgttgtgaat
     8521 agatataacg taaatccgta tcaggcaagc tatcccagtc gctgggcttg actaccttta
     8581 tagccgaaac ctttttactc ttggagggct tttctagttc tgacactacc gtttcgcctt
     8641 ccatcaacca ctgcaaaaca agaggcagta acttttgtct tgtcatcgtt ggattctggc
     8701 taaacgcttt tttaatagtg ggaattaaca ggcttgaatc gtaatcctct ttcagcatca
     8761 gatataaatg acgctgatct tcggtatcta actcttgaag ggctttttct tctggtgttt
     8821 gttcttgtat cgctgcgata tctagctgaa gttggattaa ccgtttatca atctcgggaa
     8881 cgactagatc agaataacga gcaatgcgtt ctgggatacc cgagcgtaat gcctctagca
     8941 tggggtgcac aggctttaac tcatcttgat acacttgccg caataaacca gcggtaatac
     9001 gctcattgcc taaagctagc gcacggagct gagcgagtac aaaaagtttt actacaatgt
     9061 ccatcactcc ttggcttagc tcataccaca catcacggac ctcatccgat aacagcgcat
     9121 ctttgcgttg taaaagctgt aattgccaaa gattatccgt aaaagcgatc cactcttgat
     9181 tgggctttcc acgttgcgtt tgttgtatag gatcccagaa tatagctcca aaccctgccc
     9241 ctctacgtgc agaccgcaaa tcagcctcaa aaatctctcg tgctttaggg gtaccaatca
     9301 acatcactgg tacgccaata atattcacca tcgtcacaaa aaagttcagc atctcttgag
     9361 atccacccga acgagagcgg cttaaatgct gaatttcatc aataaccaac aaccctaaag
     9421 catgtgcatt ggctatttgc gacatcaaag ccaacatggt ttctatacca tgacgtttta
     9481 agccataacg acgctcatag ttcgagccca aggctcgatc caacgctctg aaaaaattca
     9541 agcagatttc ttttagcgaa ccattatgcg agcagtctat tttcaaatac accacctgct
     9601 ctacattgag ttcacgatgg taaatcacct gaggatacgt ggctagaata cgatgaagag
     9661 aggtcgtctt cccactacca gaacaaccaa ttaacaataa gctttgtgcc gtagatcgtg
     9721 cctcctcaaa gcgaaatgtc tccaactctc ccgtttgaac acgctcataa ccattttgta
     9781 aatgcttttg taaatctcct gttttaggat ttctgcctac gtagccacct cgaatcatga
     9841 ccgaaatacg ctcacttagt agcaaatgcg tacctaatgg ctgaaaatag tcatctggaa
     9901 tacgacaaat ggtatgagct ctgataacac gggacttttg caagtcagag gaagtaagct
     9961 gtaaagagga tttcagtgat gcagcactat tcactgactc ttgtaatggt ggtaaggcct
    10021 cgataaaagg attatcacga taagcctcta cccccgtatc acgataaact gcttgaatcc
    10081 gggtagcact catgactcat ccttttctgg tggatcctga aataattcag gcacgtatgt
    10141 gggtaggctg taatcttctt gatcatccgc ttccactgcg ttgaaaggaa taactttagc
    10201 ctcatcacct gaagagcttg gcttcaaatg ctccgcacgt tttttacgct ccgaggtcac
    10261 ggcttctttt ttattagttt taatctgctt aatgcgtgtt gatttgggct cagtagtact
    10321 gggcgttaac ttattcgctt tctgaatggt ttgctgaata aacgcctcaa gctccctgcg
    10381 tttagttaac tcatcctgct tcgcattggc tttattgtgt ttttcttgtg cttgtatatc
    10441 ccaaacctcc caaaatgaga gacctttaaa ctgccgacta cgttccgtca gattacagcg
    10501 ccaaaataca cggctgccaa cttgcggaaa caaataaatc gtatcaacca gcactgggtc
    10561 ataagccgct tctaaatgtt gaggtctagc tatatcagtg ctccgctgca accaaccctc
    10621 acgcagaatc tctgaccccg agtaatacaa accccacaaa ttaacgccaa atgaagaaat
    10681 agagaccttt cggcgaggca gtaacgctac tcgcaactgc tcttgctcca cagcccttaa
    10741 actacctgta cgatgctgca taccccattg ccatagctgg acaggaatag acggtaaatc
    10801 tgtaggaaaa tcagcatctc gatcgtattt atccatcacc agatggttat ttctgaataa
    10861 gatcgtacgc aaaataattt gtgtgaactc aaataccgac agagatgcat ctaacctata
    10921 gtctgtttca ccatggcttt tgatccgact gccctctaca atgccaggtg caaaggactt
    10981 aaactcggct tgtagtgttc taaaagtgct ttccactatg cctttagcat cgccacgtct
    11041 aggtggagca ctttccactc gcacattaaa actagaaact aaggcttcga cctgatgact
    11101 cattaattcg ccacggtccg ctagcaacac atctggcaaa cctacacacg gccagtctga
    11161 gctactaatc tcaatatcat gctgggcaca aatggccgtt ttgtcagagc aagcatttac
    11221 aaaagcctgc atcgccacca cataagacgg attttcaaag ccgatataaa agcccgtgat
    11281 catccgacta aacacatcaa tcacaatgta aagcgttggt cttcctatga ttttttggcg
    11341 atcatgatga tccactaaat aaatatcagc aatcgtggca tcaatctcat aacgactccc
    11401 agggcctaac gcctgagaag tggctgtact acttaagggt cgtacgtctt ttttatatac
    11461 ccctgcttta actctagact ttaagcgctg agctttaggg tattctcgat cataaaaata
    11521 acgaaactga cgtagtgttg ggtaatcctc ttggggaatg cgaggaaaat actgagcaaa
    11581 caagtccaca aatcgtctat aggcaacggt ggtctttgta cctttttgat ttaacaggtg
    11641 cttttctatg gtcaacctaa aaaggcgttc aatctcgggc gttaccttgg ttccttcacc
    11701 cttaccatat tctctggctc ggccaatctt tgctgttcct gtcgctgaac gtctttcccc
    11761 tggtgcaccg ctgtttttgt agtcaggaat taatgcatta ggcgtttgac cacgctgcca
    11821 gtaacggcgt aacaacttat aaactgtagc cttagtaacc ttatgttctt ggaccacatg
    11881 ctcaacgagt tcgcttctga ctttagggtc gaaacgatcc ttactattaa taataggaag
    11941 aatttttcga taatcctcct cgcgcttctg gaagctgaca gaatctacag acggctcttc
    12001 taaatcaaga tgtacataag ggtcatcagt tctaactaag cggccttcat ctaaatactg
    12061 cattagcaac aactcagccc tagcttgtgg aactccttta tcagcgctta tttgcatcca
    12121 gacaacttgg ccatcctcta tagccaaaat gcgatacgga tcattatcaa atagcacaac
    12181 ctcattaatt tgccacatag cgcaactcct ccatattcac tacttggcta atacagagat
    12241 ctgcacactt atttgccctg aaagacttat aaatattgaa cttaataaaa ccatttgcgg
    12301 ttaaggctcg tatctcactc aatgttttgc ctaactccaa atcataagca atatcaacct
    12361 gcttacagac attgataatg ttttcatctc ctttttcttg caggatatgg gccaatgggg
    12421 atagttgtgc taaaagctcc gcagaaactt cttctgtttt cactgaataa agccattcaa
    12481 tattttcttt tactacggga tttatttctt tatcagtaaa aatgaaccaa ggaatttgct
    12541 tttgctgcca atagcgacgc tctagttcta gtttttctaa ggtacgctcg tcttgtaagg
    12601 ctgctgcagg tttgacttga atagcaaact gctcaaaagg accatctttg cagtccacta
    12661 aaaaatcagt agacataacc tgatctacac cacgaataac aggatgctta ataccactat
    12721 ctattgcaat ctgcctggta tcactaggta ataaggggaa ctgctcgcgt atatctagca
    12781 cgctgctctc ccactcaaga ctgagaaaaa cagcaagctc taagtcagat agcaaatgat
    12841 ggactcgtcc cgtcttatga gaataaatac ggtgggaacg acctgaagaa ggaacttctt
    12901 gtactgttag ccatggaata tagtctttac catgcccttg gccacgcccc tctttaatac
    12961 gacgggcaat ttgcacttca gaaaatgaag agtttgcttt agccataaca aaagtccagt
    13021 atgctttttc acagcataac tggactgatt tcagtttaca actattctgt ctagtttaag
    13081 actttattgt catagtttag atctattttg ttcagtttaa gactttattg tccgcccaca
    13141 gccctttttt atctatgata gtgattactc aaccgtaacc gattttgcca ggttacgcgg
    13201 ctggtcaacg tcggtgcctt tgatcagcgc gacatggtaa gccagcagct gcagcggaac
    13261 ggtgtagaag atcggtgcaa tcacctcttc cacatgcggc atctcgatga tgtgcatgtt
    13321 atcgctactt acaaaacccg catcctgatc ggcgaagaca tacaattggc cgccacgcgc
    13381 gcgaacttct tcaatgttgg atttcagttt ttccagcaat tcgttgttcg gtgcaacgac
    13441 gataaccggc atatcggcat caataagcgc cagcggaccg tgtttcagtt caccagcagc
    13501 gtaggcttca gcgtgaatgt aggagatctc tttcagcttc aatgcgcctt ccagtgcgat
    13561 tgggtactga tcgccacggc ccaggaacag tgcgtgatgt ttgtcagaga aatcttctgc
    13621 cagagcttca atgcgtttgt cctgagatag catctgctca atacggctcg gcagcgcctg
    13681 cagaccatgc acaatgtcat gttcaatgga ggcatccaga cctttcaggc gagacagctt
    13741 cgccaccagc atcaacagca cagttaactg agtggtgaat gctttagtgg acgccacgcc
    13801 gatttccgta cccgcgttgg tcatcagcgc cagatcggat tcgcgcacca gagaagaacc
    13861 cggaacgtta cagattgcca gcgaaccaag gtaacccagc tctttcgaca gacgcaggcc
    13921 cgccagggta tccgcggttt cgccagactg tgacaaggtg atcatcaggc tgttacgacg
    13981 cacggcagat ttacgatagc ggaattcaga ggcgatttcg acgtcgcacg gaatacctgc
    14041 cagcgattca aaccagtagc gggaaaccat accggagtta taagaagtac cacaggcgag
    14101 gatctgaata tgctcaacct tcgacagcag ttcgtcggcg ttcggtccca gctcgcttaa
    14161 atcaacctga ccgtggctga tgcgcccggt aagggtgttt tttatcgcgt tcggctgttc
    14221 gtagatctct ttctgcatgt agtgacggta aataccttta tcgcccgcgt catattgcag
    14281 attggattcg atatcctgac gttttacttc cgcgccagtt ttatcgaaga tgtttaccga
    14341 acggcgagtg atttccgcaa tatcgccctc ttcaaggaag ataaagcgac gggtcaccgg
    14401 caacagcgcc agctggtcag aagcgataaa gttttcgccc atacccaggc caatcaccag
    14461 cggactacca gaacgtgccg ccagcagggt atccgggtga cgggagtcca tgatcactgt
    14521 accgtacgca ccgcgcagct gcgggatagc acgcagaaca gcctcacgca gagtcccgcc
    14581 ttgtttcagc tcccagttca ccagatgggc aatcacttcg gtgtcggttt cagaaacgaa
    14641 ggtatagcca cgcgctttta actcttcacg cagcggttca tggttttcga tgatgccgtt
    14701 atgcaccacc acaatgtgtt cagaaacatg cggatgcgcg ttagcttctg aaggttcacc
    14761 gtgggtcgcc cagcgagtgt gagcaatacc ggtgccgcca tccagaggat gttcttccgc
    14821 tgcctgagcc agcatctgga ctttaccgag gcgacgcagg cgggtcatat gaccttccgc
    14881 atcaacaacg gccagaccgg cagagtcata tccgcggtat tccagacgac gtaaaccttc
    14941 aagaaggatt tctgctacat cacgttgcgc gatcgcgcca acaattccac acatagtttt
    15001 tgattccgat ttatatcgtt gtcggtcaac ctgtatgccc gttttttcgg gcgccccgag
    15061 ccttgtagag agtggggtta tttttatagt tactgcttgt gggagggaga ttatgttatc
    15121 tcctcatccc agatgaccgg attacccggc cagaattact ttttctttac cggacgacgc
    15181 cagccttctt tctgagtctg cggcacacgg ctgatagcta atgcattttc gccgacatta
    15241 cgcgtcacag ttgtacccgc agcaatggtc gcgcctttgc ctactgttac cggggccacc
    15301 agctgagtgt cggaaccgac aaacacatcg tcgccgataa tggtcttaaa tttattcgca
    15361 ccatcgtagt tgcaggtaat ggttcccgcg ccgatgttaa cgttatcgcc aatttccgca
    15421 tcgcccaggt aagtcagatg cccgactttc gagcctttac ccagacgcgc ttttttcatc
    15481 tcaacgaagt taccaacgtg tgcaccttcc agcaactcag caccaggacg caaacgagca
    15541 aacgggccaa tagtacaggc cgctgccaga ttcgcgtctt ccacaacggt atacggactg
    15601 atttcgcaat catcgccaat cacgctgttt ttaatcacgc aaccggtgcc gattttcacg
    15661 cgatgaccga gagtcacgtt gccctcgatg ataacgttag tatcaatttc aacatcgcgc
    15721 ccgtgagtaa gcgtaccgcg cagatcaaaa cgcgccggat cgcgcagcat aacgcctgct
    15781 aacagcagtt tttcagcctg ttcggactgg taaacgcgct ccagacggga gagttgcagg
    15841 cggttattca caccttctac ttcgcttaaa cgttgcggat gaacggcgac gatttcacgc
    15901 ccttcctgat acgccagcgc aataatgtcg gtgatgtagt attcgccctg agcattattg
    15961 ttggtcagct tcgccagcca gcgtttcata tctgcgccgt tagcgatcag aatgccggtg
    16021 ttgatctcct gaatctgacg ctgctcgtcg gtggcatctt tgtgctcaac aatgccggta
    16081 actttgccat tttcacgggt gatacgtcca taaccggtcg gatcatccag tttcaccgtc
    16141 agcagaccaa tgccaccctg cggtttagca tcacgcaggc gctggagtgt ttcgacagag
    16201 atcagcggca cgtcgccgta gagcattaaa atgtcttcat catcggcaaa gaaaggtgcg
    16261 gcctgctgca ttgcatgacc cgtacccagc tgctctgcct gaagcaccca gttaaggttg
    16321 tcgtctttca gcgcctgttt aagcagatcg ccgccgtgac cgtacaccaa gtgaacgtgc
    16381 gctgcgccta attcattcgc agcatcaatg acatgctgaa ccatcgcttt cccggcaagg
    16441 gtatgcagca ctttcggaag atcggaatac atgcgcgtgc ctttgcctgc ggcaaggatc
    16501 actacgctca tagcattatt caacatacgc gtcctgactg taatttgaga acgaatttaa
    16561 accgcttcac cttgaaaaaa ctacattttt ttcatcgtga aatggacaga ggataaattg
    16621 ttcaataacg gattatcccc gctgacgaaa gtgccatttt cgaccatcgt cacgcctttt
    16681 gtctgcagaa aattaaggca gtaccttcat ttaagcgtca ggtggatgtt tttgctctta
    16741 tttcgatcaa tgaataaacg ggaaaacagg ggtgttttat atcagcagga tctatgtgaa
    16801 cgctcttcag gacggatcac gcgtaaaaaa aagccagcct gtttccagac tggcttttgt
    16861 gcttttcaag ccggtgttac atcgcttttt tggtcaactc gataacgcgc agctgcgcga
    16921 tcgctttggc cagttccgca gacgcctgag cgtaatctac gtcgccgtga gagctgctaa
    16981 tgtgctcttc agccttacgt ttcgcttcca tggctcgcgc ttcgtcgaga tcctggccgc
    17041 gaattgcggt gtcggccaga acggtcacgt tgccaggctg cacttcaaga atgccgccag
    17101 acagatagat aaactcttcg tgaccgtgct gtttcacgat gcgaatcata ccaggcttaa
    17161 tggcggtgag cagcggtgcg tggccagggt agatccccag ttcaccttcg ctacccgtta
    17221 cctggatttt ctcgaccaga ccagagaaca tttgttgctc tgcgctgacg acgtccaggt
    17281 ggtaagtcat tgccatatca ccctccgatt aaggcgttaa agttttttgg ctttttccac
    17341 agcttcttcg atggaaccga ccatgtagaa cgcctgctcc ggcaggtgat cgtattcgcc
    17401 ttccatgatg cctttaaagc cacggatggt gtctttcagg gagacgtatt tacccggaga
    17461 accggtgaat acttctgcca cgaagaacgg ctgggacagg aagcgctgga tcttacgagc
    17521 acgcgctacc accagtttgt cttcttcaga cagttcatcc atacccaaga tggcgatgat
    17581 gtctttcagt tcctgataac gttgcaggat ggactgaacg ccacgcgcgg tgtcgtagtg
    17641 ttcctgacca accaccagcg ggtccagctg acggctggtg gagtccagcg ggtcaacggc
    17701 cgggtagata cccagagacg cgatctgacg gctcagtacc acggttgcgt caaggtgcgc
    17761 aaaggtggtt gccggagacg ggtcagtcaa gtcatccgca ggtacgtata ccgcctgtac
    17821 ggaggtgata gaaccagttt tggtggaggt gatacgttcc tgcagaacgc ccatctcttc
    17881 cgccagggtc ggctgataac ctaccgctga aggcatacgg cccagcagtg cggatacttc
    17941 cgtaccggcc agggtgtaac gatagatgtt gtcaacgaac agcagaacgt cacgaccttc
    18001 gtcacggaat ttctcagcca tggtcagacc ggtcagagca acgcgcagac ggtttcccgg
    18061 cggctcgttc atctggccat acaccaggga tactttgtcg ataacgttgg agtcggtcat
    18121 ttcgtggtag aagtcgttac cctcacgagt acgttcacct acgcccgcaa acacagagta
    18181 accggagtgc tcgatcgcga tgttacgaat gagctccatc atgtttacgg ttttacctac
    18241 acccgcacca ccgaacagac caactttacc gcccttagcg aacggacaca tcaggtcgat
    18301 aactttgata ccggtttcca gcagttcctg agagtttgac agctcttcgt aggaaggtgc
    18361 tgcgcggtga atcgcccaac gctcttcttc accgatctcg cctttcatgt cgaccggttc
    18421 acccagtacg ttcatgatac ggcccagagt cgctttacct accgggactt cgatcgggtg
    18481 ttcgaggtct tttacatcca gaccgcgacg cagaccgtcg gaggaaccca ttgcgatggt
    18541 acgcacgata ccgccgccga gctgctgctg aacttccagc accagacgct cattaccatt
    18601 ttgcacctca agagcatcgt acacgcgcgg tacggcatcc tgagggaatt cgacgtcaac
    18661 tacggcgccg attacctgga caatctttcc agtagccatc ttaaatcctc tacgaaataa
    18721 cctgtttaaa ccgcggcggc ccccgagacg atctcggtga gttcctgagt aatgctggcc
    18781 tgacgagctt tgttgtatac caactgcagc tctttaatca ggctgccgcc attgtcggtc
    18841 gcggctttca tcgccaccat acgggcggcc tgctcgctgg ccaggttttc aaccacgccc
    18901 tgataaacct gagattcgac ataacgacgc agcagggtat ccagcaacgc cttcggatcg
    18961 ggttcgtaca ggtaatccca ggatttatgt ttcagatcat catcatctga tgccggtaac
    19021 ggcagcagct ggctgatggt cggaacctga gacatggtgt taataaattt gttgctgaca
    19081 atgtaaagct tgtccagacg gccttcgtcg taagcctgca acatcacttt taccggaccg
    19141 atcagttcgg acagggaagg gttatccccc atgccggtga cctgggcaac aacattgccg
    19201 cccacggagt tgaagaacga cacgcctttc gagccgatca ttgcgaggtc gcattgaacg
    19261 cttttgtcgg tccaggtctt catttccgcc agtagttttt tgaacaggtt aatgttcaaa
    19321 ccaccgcata aaccacggtc ggtcgacacc accaggtagc ccacgcgttt aacgtcgcgg
    19381 tcttccaggt aagggtgctt atattccaga ttaccgtgtg caaggtgacc aatcactttg
    19441 cgcatggttt ctgcataagg acggctggcc gccatgcgat cctgcgattt acgcattttg
    19501 gaagcggcga ccatctccat cgctttagtg atcttttgcg tgttctggac gcttgcgatc
    19561 ttactacgta tctcttttgc gccggccatg agcttctcct caatgccttg cggcctgccc
    19621 taaggcaagc cgccagacgt taccaggatt gggttgcttt gaaggaatcg aggatgcctt
    19681 tcagcttgcc ttcgatttcg tcgttgtagc caccggtctg gttgatctct tgcatcaacg
    19741 gagcgtgatc acggtcgacg taagccagca gagcggcttc gaagctgcca attttcgaca
    19801 gttcaacatc cgccaggtaa ccacgttctg ctgcgaacag aaccagagac tgctgcgcaa
    19861 cggacatcgg cgcatactgt ttctgtttca gcagttcggt cactttctga ccgtggtcaa
    19921 gctgcttacg tgttgcatcg tcaaggtcgg atgcaaactg agagaacgct gccagttcac
    19981 gatactgtgc cagagcggta cggataccac cggacagttt tttcatgatc ttggtctgtg
    20041 ctgcaccacc aacacgggat acggaaatac ccgggttaac cgcaggacga ataccggcgt
    20101 tgaacaggtt ggtttccagg aagatctgac catcggtaat ggagattacg ttggtcggaa
    20161 cgaacgcaga aacgtcaccc gcctgagttt cgataatcgg cagtgcggtc agagaaccgg
    20221 ttttcccttt cacttcacct ttggtgaagg cttcaacgta ttcggcgtta acacgtgcag
    20281 cacgctccag cagacgagag tggaggtaga aaacgtcgcc cgggaatgct tcacgtcctg
    20341 gcggacgacg gagcagcagg gagatctgac ggtaagcaac agcctgttta gacaggtcat
    20401 cgtaaatgat cagcgcatct tcaccgcggt cacggaagta ttcgcccatt gcgcaaccgg
    20461 catacggtgc caggtattgc agtgcagcgg attcagacgc ggttgctacc acaacgatgg
    20521 tgttagccag tgcgccgtgc tcttccagtt tacgtaccac gttagaaatg gtggacgctt
    20581 tctggccgat agcgacatag atacatttga taccggaatc gcgctggttg atgatggcat
    20641 cgatagccag tgcggtttta cctgtctgac ggtcaccgat gatcaattca cgctgaccac
    20701 gaccgattgg gatcatggag tcaacggctt tataaccggt ctgtaccggc tgatctacgg
    20761 actgacgttc gataacgccc ggagcgattg cttctacagc agagaagccg tcgtgatcca
    20821 gcggaccttt accgtcgatt ggtgcaccca gagtgttaac cacacggccc agcaggccac
    20881 ggccaaccgg aacttccagg atacggccag tacacttaac tttcatgcct tcggcaaggt
    20941 cagcgtacgg acccataaca accgcaccta cagagtcgcg ctcaaggttc agtgcgatag
    21001 cgtaacggtt acccggcagg gagatcattt caccctgcat acaatcggcc aggccgtgaa
    21061 tgcggataac accgtcactt acagaaacaa tagtaccttc gttgtgagct tcactcacaa
    21121 cattgaactg agcaatgcgc tgcttgatca gttcgctgat ttcggtggaa ttcagttgca
    21181 tgctccagtc cccttaagac tgcaagacgt ctgcaaggcg ctcaagacga ccgcgtacgc
    21241 tgccatcaat gaccatatca cccgctcgga tgataacgcc tgccattaca gacttatcga
    21301 ttttgcaatt cagcttaact ttgcgtgaca gacgtttttc catcgcagca gaaattttcg
    21361 cgagctgttg ttcactcagt gcggcagcgg aaatgacgtc tacctcagcg gtagcctcac
    21421 tcacggcacg aaggtgaata aactgctcca gaacatccgg gagcgcgtta agacgaccat
    21481 tttcagccat tacccgaatc aggttctgac cgttttcgtc cagttgctca ccacaaactg
    21541 cgataaacga ctcggcgagc gtttctggcg caagcgcgcc agagagaagc tctgccattt
    21601 gttcgttttt ggttacctcg gcggcaaacg ccagcatgtc ctgccagcgt tctacacttt
    21661 ggtgttcgac ggcaaagtca aaagctgctt tggcgtaggg gcgagctacc gtaataaatt
    21721 cagacatcag cccctccctc cttacagttc agcgacaagt ttatccacga tgtcgctgtt
    21781 agcagcttca tccacggaac gttcgatgat cttctcggcg ccagcaacag ccaggatagc
    21841 aacttgctta cgcagctctt cacgggcacg tttacgctcg gcttcaattt ctgcctgcgc
    21901 ctgggccacg attttagtac gttcctgttc tgcctcagct ttcgcttcgt ccagaatctg
    21961 cgagcggcgt ttgttcgcct gctcgatgat tacctgggct tccgctttcg cttttttcag
    22021 ctggtcggtc gcgctggcct ttgcaaggtc aaggtcctta tgtgctcgtt ctgcggaagc
    22081 aaggccgtca gcaatttctt tttgacgttt ttcgatggct gccattaatg gcggccatac
    22141 gtacttcatg cagaacagaa cgaacaggac aaacgcgatg gcctggccga ggattgttgc
    22201 gttaagattc acagcacaat gcctctattt agttaacgtt ctgatattgc tctttaaata
    22261 aaagcaacgc ttactacgcg acagcgaaca tcacgtacag acccagacct acagcgatca
    22321 tcgggatagc atccaccaga cccataacga taaagaactg agtacgcagc agaggaatca
    22381 gatcaggttg acgcgctgcg ccttccagga atttaccccc gaggatgccg ataccgatcg
    22441 cagcaccgat tgccgccaga cccatcatca cagcggcagc catgtacagc agatccatat
    22501 tcaggttttc catgacagtc tccagtttgt ttcagttaaa acgtagtagt gttggtaaat
    22561 taatgttctt cagacgccat cgacagatag acgatcgtca gaaccatgaa gatgaaggct
    22621 tgcagcgtaa tgatcaggat gtggaaaatg gcccacggca cattcaggat ccactgtgac
    22681 caccacggca acagaccagc aatcagaatg aaaatcagct caccggcata catgttaccg
    22741 aacagtcgca aaccgagtga aactggtttg gacagcaggc ttaccccttc aaggattaag
    22801 ttgacaggaa tgaacgccca gtgattgaac ggctgcagcg tcaactcttt cgtgaagccg
    22861 ccgatgcctt tcattttgat gctgtagaac agaatcagga taaatacgcc cagtgccata
    22921 gacagcgtta cgttcacgtc cgcagacgga accacacgca gtgcaggcag acccagtaca
    22981 tgttcagcaa tgtacggcag caggtcgata ggcagtaaat ccatcaggtt catcaggaat
    23041 acccagacga agatcgtcag ggccagcgga gcaatcagct tgcttttgcc atggtacatg
    23101 tctttcacgc taccattaac aaagccgatc accagctcaa tcgcggtctg aaacttacct
    23161 ggcacaccgc tggtcgcctt tttggctacg ctacggaata aaaccaggaa caacagaccc
    23221 agcaccaccg agaagaacat ggagtcaata ttgattgtcc agaaggtggc tggggggttt
    23281 tgtggatcca ccagcgagaa tgtacgcagg tccagctgaa ggttattcag gtggtgtcct
    23341 atgtaatcct gcggcgtcat attttctgaa gccatgatgc cttttaccct ttgttgttaa
    23401 ttacagccgg tgccagtatc tgaaccacca gcaccaaaac ccacgtaacg atcagcggca
    23461 agaataccgc ctttaaaacc gccaacgcca ccaccagtaa caccaacatc gccagaactt
    23521 tgaaagcttc gccaaatgcg aatgtccagg ccacccggcc tttcgctggt gtatacgcct
    23581 ggtgacgcca ggcaaatatc ataaacaaaa cgttaggcag aaagactgcc aggcccccgc
    23641 ttattgcaga gacgccccag aaggggtctt tgaggctgaa cagcaatcca cttgctatca
    23701 ccaccagtaa ctgaacgagc agaagcttcc gagcaacgtt tcgactcacg agcgacacag
    23761 acatcacgtt tttcactcct gctcccttcg aggtatgccg cgtgtcgtat aaaactttct
    23821 ttaaggctta gagtcaagca tcaaaaagcg gtcaaattat acggtgcgcc cccgtgattt
    23881 caaacaataa gtagccaaaa ggtgaataaa tgtttaaata tttttccagt gcatacaatt
    23941 gcgacttttc cgctaaccct gttcgatcat gaaaaactgt aaataacgcg taaacactag
    24001 tgataaagcg tgcttcagat cacatattgc gcatgttcgc gcacagtata tttatttact
    24061 tggcaaatga tgcctttgca agtttatgat atttcagtct aaaaacagat actgttttaa
    24121 taaatgacat ttacacaaca aaaaccaccc attgacattt ttaataatgt tttaacagcc
    24181 aatgatggtt cttagcgccg atttttagca gactgatatt ttcactaatg acttattttc
    24241 tgcttaccaa aaaaagccac gttatcttgt tgatacaaaa gagtgaacgt ggcgttaaat
    24301 gtaaccagtt atatcagtag aaaacttggt tgttgttaac agtctaaccg gtcaattttt
    24361 tatgattttt ttgataaaaa ttaaatttta tttgctttaa tcaccaccag atgacgttcg
    24421 ccatccagag ctggaacctg aagtttaacc actgattcga cctgatattc ttcgggcaac
    24481 aaagcgattt catcttccgg catttgccct ttcagcgcgt agaaacggcc ttgctcacca
    24541 ggaagatggt ggcaccagct caccatatcg ttcagagagg caaaagcgcg gctaattacg
    24601 ccatcaaatg gcggctctga aggaaactct tctaccctgc tctgtactgg ctcaatattc
    24661 tccagtttaa gctcatgttg cacctgacga aggaaacgca cgcgtttacc aaggctatcc
    24721 aacagagtga aatgggcttc aggacgcacg atagagagtg gaatgcctgg cagtcctggt
    24781 ccggtgccga catcgataaa ccgttcacct tgcagatacg gtgccaccac aatgctatcg
    24841 agaatatggc gtaccagcat ctcattagga tcgcggaccg aagtcaggtt gtacgctttg
    24901 ttccatttat gcagcatatt cacgtaggca ataagctggt ttttctggtg atcggtaagc
    24961 gaaatacctg cgtctttcag cagtaaggag agtttgttga gcacggtgat tacctgttct
    25021 tgatgcgttg cctggtaagc gggtgcttac caggcatttt taatgcgtta tgcgctacga
    25081 cgcagcatac cctgtttttt cagccacacc agcagaatgg agatagccgc aggcgtgacg
    25141 ccagaaatac gcgaagcctg gccgatagag gccggtttgt gatcgttaag tttggcgatc
    25201 acttcgttag aaagaccgga tacctggcgg taatccagtg tcgcgggtag cagggtgttc
    25261 tcgttacgca gctgcttttc gatctcatct tgctggcgcg cgatataacc ttcgtattta
    25321 acctgaatct caacctgttc cgccgcctgt tcgtctgtca acgcaggggc aaacggcgtc
    25381 agcgtggtta atttttcata agtcatttcc ggacgacgca gcagatcttc accactggct
    25441 tcacgggaaa gcggcgcagt caggtgagca ttcacttcgg ctgcagcttc cgccgacggg
    25501 gttacccagg tcgatttcag acgctgacgc tcacgctcga tattctcaag tttctcgtta
    25561 aagcgcgccc aacgttcgtc atccaccagg cccagttcac gaccgatttc agtcaaacgc
    25621 agatccgcat tatcttcgcg cagcatcaga cgatattctg cgcgcgaagt aaacatacga
    25681 tacggttctt tggttcctaa agtgcacagg tcatcaacca gtacgccgag atacgcctga
    25741 gaacgcgccg gagcccaacc ttctttgtca gcagacagac gggcagcgtt aagaccgacc
    25801 agcaaacctt gcgcagcggc ttcttcgtaa ccggtagtgc cgttaatctg accagcaaag
    25861 aacagcccct ggataaactt gctctccagc gtcggtttca ggtcgcgtgg atcgaagaag
    25921 tcatactcaa tggcataacc cggacgcacg atcttcgcgt tttccatccc ctgcatggag
    25981 cggacgattt gcatctgcac atcgaacggc aggctggtgg agataccgtt cggataaatt
    26041 tcgttagagg tcagcccttc cggttcaagg aagatctgat gctgatttct gtcggcgaag
    26101 cgcatgactt tgtcttcgat cgatgggcag tagcgtgggc cgacaccttc gatcacccct
    26161 gcgtacattg ggctacgatc gaggttactg cggatcacat catgggtttt ctcgttggta
    26221 tgagtgatat aacacggcac ctgctgggga tgctgggacg cattgcccat aaacgagaat
    26281 accggcattg ggttatcgcc atgctgttgc gccagcacac taaagtcgat agttcgagca
    26341 tcaatacgcg gtggtgtccc ggttttcaga cgaccaacgc gcagcggcag ttcacgcaaa
    26401 cggcgagaaa gcggaatgga cggcggatca ccagcacgac caccgctgta attatccaga
    26461 ccgatatgaa ttttaccgtc gaggaacgtc ccaacggtga gcacgacggc tttggcacgg
    26521 aacttcagtc ccatttgggt aacagcaccg accacgcgat cgttttcgac aataagatct
    26581 tcaaccgcct gctggaagat catcaggttc ggttggttct ccagcgccgt acgtaccgcc
    26641 tgacggtaga gcacacgatc cgcctgagct cgggtagcgc gaaccgccgg tcctttgctt
    26701 gcgtttagta tcctaaactg gatacccgct tgatcgatcg ctttcgccat cagaccgccg
    26761 agtgcatcca cttcttttac cagatgtccc ttcccaatac cgccgatcgc cgggttgcag
    26821 ctcatctgcc ccagagtgtc gatattgtgt gtcaaaagca gagtctgttg acccatacgc
    26881 gccgcggcca tcgcggcctc ggtgcctgca tgacccccgc caatgatgat gacgtcaaaa
    26941 ggatccggat aaaacatggt gattgcctcg cataacgcgg tatgaaaatg gattgaagcc
    27001 cgggccgtgg attctactca actttgtcgg cttgagaaag acctgggatc ctgggtatta
    27061 aaaagaagat ctatttattt agagatctgt tctattgtga tctcttatta ggatcgcact
    27121 accctgtgga taacaaggat ccggctttta agatcaacaa cctggaaagg atcattaact
    27181 gtgaatgatc ggtgatcctg gaccgtataa gctgggatca gaatgagggg ttatacacaa
    27241 ctcaaaaact gaacaaccgt tgttccttgg ataactaccg gttgatccaa gcttcctgac
    27301 agagttatcc acagtagatc gcacgatctg tatacttatt tgagtaaatt aatccacgat
    27361 cccagccatt cttctgccgg atcttccgga atgtcgtgat caagaatgtt gatcttcagt
    27421 gtttcgcctg tctgttttgc accggaattt ttgagttctg cctcgagttt atcgatagcc
    27481 ccacaaaagg tgtcatattc acgactacca ataccgattg cgccaaagcg gaccgcagaa
    27541 agatcgggct tctgttcctg caatgcttca tagaaaggag aaaggttgtc cggaatatct
    27601 ccggcaccgt gggtggagct gataatcagc cagatccccg aggcagataa atcttccaac
    27661 agcggaccgt gcagcgtttc ggtggtaaaa cccgcctctt ccagcttttc agccaggtgt
    27721 tctgctacat attcggcacc gccgagggtg ctgccgctga taagagtgat atctgccata
    27781 aaccgccacc tttattaaga gtggcgtatt gtacgctgtg aacgcgttgg gatctacctg
    27841 tggaaaagta tgggattaaa aaagccgatt atggcttgat ggtacgcatg atcgggtttt
    27901 gcaggacgat caatgtctcg gtggactgaa tttcatcaat tgtttggatc ttgttgataa
    27961 gtacatgctg gagagcgtcg atcgaacggc acattacttt tataaagatg ctgtagtggc
    28021 ctgttgtgta ataggcttca gtgacttcag caaggctttc cagctttgcc agcgcggaag
    28081 ggtagtcttt ggcgctcttt aatataatgc cgataaagca gcctacgtca taaccgagct
    28141 gcttcgggct gacatcaata cgcgccccgg taatgatccc cgcctgcttc attttctcta
    28201 ctcgaacgtg aatcgtcccc ggactgacgc caaattgttt cgccagttcg gcgtaagcgg
    28261 tgcgcgcatt gcccattaat gcttccagga tgccacggtc cagattgtcg atcagataat
    28321 tttccatagg attttcttat gcagattgat gattcattct attttagcct tcttttttaa
    28381 tgaatcaaaa gtgtgttagg ctttttattg aatgattatt gcatgtttgt cggtttttgt
    28441 tgcttaatca taagcaacag gacgcaggag tataaaaaat gaaaaccgct tacattgcca
    28501 aacaacgcca aattagcttc gtgaaatctc acttttctcg ccaactggaa gaacgtctgg
    28561 ggctgatcga agtccaggcg ccgattctta gccgtgtggg ggatggcacg caggataact
    28621 tgtcgggctg tgaaaaagcg gtgcaggtaa aagtgaaagc tctgcctgat gcccagttcg
    28681 aagtggttca ttcactggcg aagtggaaac gtcagacctt agggcaacac gacttcagcg
    28741 cgggcgaagg gctgtacacg cacatgaaag cccttcgccc cgatgaagac cgtctttctc
    28801 cgttgcactc ggtctatgtt gaccagtggg actgggaacg cgtaatgggc gacggtgagc
    28861 gtcaattctc gactctgaaa agcacggtag aggcgatctg ggcgggaatt aaagcaaccg
    28921 aagctgcggt tagcgaagag tttggcctgg caccgttcct gccggatcag atccacttcg
    28981 tacacagcca ggagttactg tctcgttatc cggatctcga tgccaaaggg cgtgagcggg
    29041 cgatagcgaa agatcttggc gcggtattcc ttgtcgggat tggcggcaag ctgagcgatg
    29101 gtcatcgcca cgacgtgcgc gcaccggatt atgatgactg gagcaccccg tcagagctgg
    29161 gtcatgcggg tctgaacggc gatattctgg tgtggaaccc tgtactggaa gatgcgtttg
    29221 agctttcttc catggggatc cgcgtggatg ccgacacgtt gaagcatcag ttggcgctga
    29281 ccggtgatga agatcgtctg cagctggaat ggcatcaggc gctgctgcgc ggtgaaatgc
    29341 cgcagaccat cggcggcggt atcggccagt ctcgtttgac catgctgctg ctgcaactgc
    29401 cgcatatcgg ccaggttcag tgtggtgtat ggccagctgc tgttcgcgag aacgtccctt
    29461 ctctgctgta ataatttatc gccgccagcg tctgagcagg cggcttcgca tcccggtatc
    29521 aaagcgccag atatgatcga aaatgcgcat gatgccgggt ttgccgtgtg ccgacatcgc
    29581 cacggcatga aagcgatgct gatgtacccg ctgcagctct ttcactttac tcgtcacgtc
    29641 gtcaggcaac cgctgagcga taaaatcaga aatcaccacc gcatcggcat caaaccattc
    29701 cctgctttgc aagcgttcca taatggcgcg aaaacaactg gcaagatcgg tgccgccacg
    29761 aaactgctgg cttaaaaaac ggattgcttg ttcgatgcct tgggggcctg aaagctcata
    29821 acggacgatc tcggtggaaa atagcataat atagcagcgc cggttttcag cgagagcaat
    29881 gcgcatcaag gccaggcaga acgctttcgc acactgttca ttaaagccgc ccattgagcc
    29941 ggaagtatcc acacagacaa taaacggccc gcgcggctgt tcatcgtaat ctttatgtac
    30001 caccggacgt tcgatcactt tttcacgcca cgactcaccg tgcaggcgat aggtgagcaa
    30061 ctgtttttcc accagccgac ggtaaaactc atactccagt tccgttatcc ctagtgtcgc
    30121 cagttctggc ggcagaagac gtaaaatatc atcgctttgt tgcagaccat caacctgctc
    30181 aggaaccgtc gccggttcgc gcaccatggt gcggaaggtt tccatctgcg catcgttgcg
    30241 cggtattgat ttggcttccc gagaacgccc cagctgctct gccaggcgtt tcagttccgg
    30301 ctgttcgtta agaaattcac cgtatttcac aatcaactga tagtcgccac gtttaagctg
    30361 accggcgctc atatcccaca gacgaccagc tgcagtattg ttatctgcga gaatcggttc
    30421 aagttgtccg ctcagcgtca tgcgttcttg aacttcactc aacagttgtt cgcgttcttc
    30481 ttctaatagc tgttgattta acgtcgttgc ttgcacgatc agacttaaac gccaacgctg
    30541 gagaaaaagc gtgtgtaacg ctgaagttat tgtgctgtta gcatcaacca actgccgggc
    30601 ttgttctgcc catggggaat tcagacgatg cagtaagtcc aggatctgtg gtagctgcac
    30661 aataaactgc ggcgtggaga ggagctggct ttgctgatag cacatcacct cttcggtgag
    30721 ttccggcggg actcgggcat ctttcagccg actgcgcagc gcctcacgcc agcggggaac
    30781 atcatcagtg attgccgcct tcaggcgtgg gaatttttca aagaagaccg ccagctgcgg
    30841 tgaggccagc agtgcgatga tcatctcttc gatcaatccc tcttcgctga cggccagcat
    30901 cacattaagc gtatccagcg ttagcattgt tgtgcctggt gaatctgtgc accgacatcc
    30961 tgtaggctgg cttcaatgcg acctaaccag tcgccaggga taaacagaca tttttgctgt
    31021 tcgctgaata aagcgtgttg cttgcgccag tcgctttcaa gctcttccag ttgttgtttt
    31081 atttcaccag gcagaccttc agccgatgaa ccgggaagtg ccagcgtact gccttgtaaa
    31141 cttacatcgc gaacgacaag atgttgggcg ctatcaactt ccagattcag tttctgggca
    31201 aagccgatac cgttcagttt accgcgaatt tcaccacctt tgctcagcca ctgttccagc
    31261 gcgctacgct caaaggagat atgaaccact tccatatcat gcagttttaa cggtttttgc
    31321 agcagtagag tcagagtgga agcagtaacg ttaacaggaa gttgatactg ctgacgacgg
    31381 ctgaaaatgc cgcccagacg aattaccgtt aaggctgttt tatcgctttg ttgctgctgg
    31441 agttgcaggt gacgttgcac aatcgcgcca aggcgagtca acatcccttg ctgttgccag
    31501 gcatgaccgg tcatcaatac atcaatttgt tgttgtatca aattcaggct ttgcgcgtca
    31561 taccacaggc aatctttcag caaaatgaga tctaccgggg caacagcact gcgaccgcta
    31621 aaaaaggcgc tggcctgcaa taatcggatc gcttttttcc agcgacgatc cgagacataa
    31681 ggcgcatccg gtaatttatc cagttgcagg cgcagcataa aaatcagctc aaatacatga
    31741 tcgggcagcg taatttcacc aatctctttc tgccagcgtt catattcttc atctgtgacc
    31801 tgcaaggcat caggaaccgg attgtcgttt tcatcctgtt gactggtcag catggagcgg
    31861 aaattcgctt tatcctgcac tttatctaac cacagacgaa tcagcatgcg gtcatataac
    31921 gcttccagac tgctgtctgc ttccggcagc tcgttggagg ccgccaccag cagacgcatc
    31981 gggatttttt ctacgtgtgc accgttgcgg aactggcgct cgttaatggc ggtgagcaag
    32041 gtattaagaa ttgccgggcc cgctttccag atctcatcca gaaagacaat ttctgcttcc
    32101 ggcaggtaac cgctggttaa acgttcatag cgcccttcat cttttagcgc ctgaatagaa
    32161 aggggaccaa aaacttcttc cggcgtggag aagcgggtca tcagatattc aaacgcgcgg
    32221 gcattctgaa aggcgaattt taagcgccgg gcgatcaaac ttttggcaat acctggcggg
    32281 ccaaggagga acacactttc accacttaat gccgctaata aacacaagcg gatggcgtgg
    32341 ctacgttcat aaagcccctt ttccaacgaa ctgctcaggc gggaaattct ttccgctaat
    32401 aaatgagggt gagccataat gaagtggcgt cctttcgtca aaagttctgc gtaaattgcg
    32461 agtatagacg tttcttgctg gtggctaaaa tagtctcaaa ggggggtaat gccgatcagt
    32521 taaggatcag ttgaccgatc ca
//
LOCUS       DADMKF010000445        16287 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.guided.089, whole genome shotgun sequence.
ACCESSION   DADMKF010000445 DADMKF010000000
VERSION     DADMKF010000445.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16287
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.guided.089"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            72..1196
                     /locus_tag="JNC24_004461"
     CDS             72..1196
                     /locus_tag="JNC24_004461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HAY9161004.1"
                     /translation="MTIHYTATLNNKHLSLIALYPDGNREVIAILPVTELGALINRNN
                     RLMHLSFSSPFREITETVLVDTVDAERPGHHIGRMLSAIDACLDAEYAGKGKHPSRKN
                     GFWRTLMCGFIAGIALPATVAGYHWMKAEPPSTLAYPTSQEWRAEPVTDTETPRPHTE
                     HHVNSIPARPAPTQQEPSSAPAPTAVTEEQHVQAAEARRNLATVLKRNADRGMFTINL
                     STGHERTLYAFLDPACINCRLLEPALKQLAREFSVVIYPVSVIGGESSTKRTAPLLCE
                     PDSQKRATGWHQLYSADSGMAPTGDAPAAQDGQCLTAARAAIDVNNQAFRQFGFGGTP
                     WVLSDTGWHLPSGILKDATSVRLFLKATDSEEYHNEPAPR"
     gene            1177..3480
                     /locus_tag="JNC24_004462"
     CDS             1177..3480
                     /locus_tag="JNC24_004462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="HAY9161005.1"
                     /translation="MSQHHVNPDLIHRTAWGNPLWNALHNLNITGLCLAGSIITALIW
                     PLALPVCLLFTLVTSVIFMLQRWRCPLRMPMTLSLDDPSQDRKVRRSLFSFWPTLFQY
                     EADETFPARGIFYVGYRRINDIGRELWLSMDDLTRHVMFFATTGGGKTETTFAWLLNP
                     LCWGRGFTFVDGKAQNDTTRTIWYLSRRFGREDDIEVINFMNGGKSRSEIIQSGEKSR
                     PQSNTWNPFAFSTEAFTAETMQSMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVREGK
                     TMSLQMLREHMTLEGMAKLYCRGLDDQWPEEAIAPLRNYLQDVPGFDMSLVRTPSAWT
                     EEPRKQHAYLSGQFSETFTTFAETFGDVFAADAGDIDIRDSIHSDRILIVMIPALDTS
                     AHTTSALGRMFVTQQSMLLARDLGYRLEGTDAQTLEVKKYKGSFPYICILDEVGAYYT
                     DRIAVEATQVRSLEFSLIMTAQDQERIEGQTSATNTATLMQNAGTKFAGRIVSDDKTA
                     RTVKNAAGEEARARMGSLQRHDGVMGESWVDGNQITIQMESKIDVQDLIRLNAGEFFT
                     VFQGDVVPSASVYIPDSEKSCDSDPVVINRYISVEAPRLERLRRLVPRTVQRRLPTPE
                     HVSSIIGVLTAKPSRKRRKNRTEPYRIIDTFQRQLATAQTSLDLLPQYDTDIESRANE
                     LWKKAVHTINNTTREERRVCYITLNRPEEEHSGPEDIPSVKAILNTLLPLEMLLPVPD
                     LTASPPHKKNVAQTPSGGKQESRKKRF"
     gene            3959..4156
                     /locus_tag="JNC24_004463"
     CDS             3959..4156
                     /locus_tag="JNC24_004463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9161006.1"
                     /translation="MGSTKNGARVPHVRNRFTSMDIGMNSQIPLYCKQDIFIFYIQWL
                     IFLLIGNTMKNTMLRKGLTIF"
     gene            complement(4243..4320)
                     /locus_tag="JNC24_004464"
                     /pseudo
     CDS             complement(4243..4320)
                     /locus_tag="JNC24_004464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013221335.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            4344..5159
                     /gene="sul2"
                     /locus_tag="JNC24_004465"
     CDS             4344..5159
                     /gene="sul2"
                     /locus_tag="JNC24_004465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HAY9161007.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            5220..6023
                     /locus_tag="JNC24_004466"
                     /note="aph(3'')-Ib"
     CDS             5220..6023
                     /locus_tag="JNC24_004466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HAY9161008.1"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASR
                     RGELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAW
                     PSMGQQLGAVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLL
                     ARVERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLA
                     LMIANAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     gene            6023..6859
                     /locus_tag="JNC24_004467"
     CDS             6023..6859
                     /locus_tag="JNC24_004467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HAY9161009.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            complement(6831..7253)
                     /locus_tag="JNC24_004468"
     CDS             complement(6831..7253)
                     /locus_tag="JNC24_004468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005001938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9161010.1"
                     /translation="MNQHVWQEKELPGYVQREFEEFLQCGRLEHGFLRVRCESCHAEH
                     LVAFSCKRRGFCPSCGARRMAESAALLVDEVLPEQPMRQWVLSFPFQLRFLFGVVCGK
                     GRNPTLRLWPAIFSGEIDVFPGDRRSRLISSMTSVAPA"
     gene            complement(7465..7896)
                     /locus_tag="JNC24_004469"
     CDS             complement(7465..7896)
                     /locus_tag="JNC24_004469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tolA family protein"
                     /protein_id="HAY9161011.1"
                     /translation="MVLRSIWIEEQYMGVKKIVMLAILVSFVAGCAPLHPSNCHKTTA
                     LGSCSSGRWDDQDEWGEQARGIRDAINAKLDDPQKWKRKKCRLHMEFSRDGTALKIST
                     SNGDKAYCEAIKSAAHKAKFPAFNNPEVYRDFQKSGFDMRG"
     gene            complement(7964..10675)
                     /locus_tag="JNC24_004470"
     CDS             complement(7964..10675)
                     /locus_tag="JNC24_004470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAY9161012.1"
                     /translation="MNAIIPHKRRDGRSSFEDLIAYTSVRDDVQENELTEDSEIKSDV
                     PHRNRFRRLVDYATRLRNEKFVSLIDVMKDGSQWVNFYGVTCFHNCNSLETAAEEMQY
                     KADKAVFSRNDTDPVFHYILSWPAHESPRPEQLFDCVRHTLKSLELSKHQYVAAVHTD
                     TDNLHVHVAVNRVHPETGYINWLSWSQEKLSRACRELELKHGFAPDNGCFVHAPGNRI
                     VRKTALVRERRNAWRRGKKQTFREYIAQMSIAGLREEPAQDWLSLHKRLASDGLYITM
                     QEGELVVKDGWDRAREGVALSSFGPSWTAEKLGRKLGEYQPVPTDIFSQVGTPGRYDP
                     EAINVDIRPEKVAETESLKQYACRHFAERLPEMARNGELESCLDVHRTLAKAGLWMGI
                     QHGHLVLHDGFDKQQTPVRADSVWPLMTLDYMQDLDGGWQPVPKDIFTQVIPGERFRG
                     RNLGTQAVSDYEWYRIRMGTGPQGAIKRELFSDKESLWGYTTVQCESLIEDMIAGGNF
                     SWQACHEMFARKGLMLQKQHHGLVIVDAFNHELTPVKASSIHPDLTLSRAEPQAGPFE
                     IAGADIFERVKPECRYNPELAASDEVELGFRRDPVLRRERREARAAAREDLRARYLAW
                     KEHWRKPDLRYGERLREIHAACRRRKAYIRVQFRDPQLRKLHYHIAEVQRMQALIRLK
                     ESVKEERLSLIAEGKWYPLSYRQWVEQQAVQGDRAALSQLRGWDYRDRRKDKRRTTNA
                     DRCVILCEPGGTPLYEDTGVLEARLQKDGSVRFRDRRNGELVCVDYGDRVVFYHHQDR
                     NELVDKLNLIAPVLFDREPRMGFEPEGSYQQFNDVFAEMVAWHNAAGITGSGHFVISR
                     PDVDLHRQRSEQYYHEYIRQQKSISGGHGASYAPVQDNEWTPPSPGM"
     gene            complement(10687..11019)
                     /locus_tag="JNC24_004471"
     CDS             complement(10687..11019)
                     /locus_tag="JNC24_004471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nikA protein"
                     /protein_id="HAY9161013.1"
                     /translation="MSEKKTRTGSENRKRIVKFTARFTEDEAEIVREKAESSGQTVST
                     FIRSSSLDKVVNCRTDDRMIDEIMRLGRLQKHLFVEGKRTGDKEYAEVLVAITEYVNA
                     LRRDLMGR"
     gene            11252..11587
                     /locus_tag="JNC24_004472"
     CDS             11252..11587
                     /locus_tag="JNC24_004472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000157082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobC"
                     /protein_id="HAY9161014.1"
                     /translation="MASKKFYSDDDIQLAKAALSELPDLTAQRKTLRDFLDAIRDDII
                     ILVRTKGYTLTDVRDTLKNAGYEAGEKALRDIIREAESKKPSRRSSSKTAPKKTDSGR
                     KDGIDMNNN"
     gene            complement(11673..12521)
                     /locus_tag="JNC24_004473"
     CDS             complement(11673..12521)
                     /locus_tag="JNC24_004473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HAY9161015.1"
                     /translation="MNQTLPTADLNTAGTTDVIPSVAIDRIIAQRNEGIALFMQAMEC
                     LATARKILLDASGDIFLYGFEDCVTDSVRRMDKTEEAKRNITRLADRKIWDRLMTDTG
                     MYTFMSSCQRDEWNSQLMSDTCPEITLDNVLATFRHLNASKMQTFEQGLIDVYRKLSW
                     DYRTNNPCRLGKKIIIENLLYRWSNGRVTLDCSGREALDDLVRPFYLLEGRNVPDFRN
                     SIGAQYGEFLGNGDNVGKLLEGEYFTVRGYQKGTVHIVFKRSDLVEKLNDIIARHYPG
                     ALPPRV"
     gene            13101..13352
                     /locus_tag="JNC24_004474"
     CDS             13101..13352
                     /locus_tag="JNC24_004474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003980153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY9161016.1"
                     /translation="MTGWELRIWRKSMLWSREKAAREFGVTQRTWHAWENAEQVDVTV
                     WRTTQALSVRDLLPHMQGMRKADIIRRLENELGETAGNV"
     gene            complement(13383..13604)
                     /locus_tag="JNC24_004475"
     CDS             complement(13383..13604)
                     /locus_tag="JNC24_004475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001348086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9161017.1"
                     /translation="MPGFFVSRSRANSMTIAERLIQKGFDEGFKQGALEVAREIACRL
                     RDIGWPLERIQEVTGLSGEELKKLFPDEQ"
     gene            complement(13628..14554)
                     /locus_tag="JNC24_004476"
     CDS             complement(13628..14554)
                     /locus_tag="JNC24_004476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HAY9161018.1"
                     /translation="MSKKKNTTTPTPHDAAFRSFLANPDVARDFLELHLPAEYRQLCD
                     LSTLKLEPATFVEPDLHQYASDILWSVKTTGGEDGYVYTLIEHQSTENLYMPFRMLRY
                     SVAAMQRHLEQHKTLPLVIPVLFYHGERSPYPYSMNWLDCFENPALAAKIYTKPFPLV
                     DITVVDDNEIMNHRRMAALTLLMKHIRHRDMMELLDKLPQVMVEISDEQVRVLIHYIV
                     NAGDSVSPEFMRALAERLPQHEDKLMTIAERLEQKGRQEGRMEGRMEGRMEGALEKAL
                     AIACQLQKMGMTPEQIKQATGLSDDELKKITH"
     gene            complement(14551..14940)
                     /locus_tag="JNC24_004477"
     CDS             complement(14551..14940)
                     /locus_tag="JNC24_004477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein CbiX"
                     /protein_id="HAY9161019.1"
                     /translation="MKSCFDTDKAFHMSFDEVTFNADRIRSKLELLSEELMPRCGLVY
                     ELYQRRLSAAIDNFVATLPTEQHLLVLELAREEFDYLSAEEIAEEIRRDSERGYCCHG
                     FERNCCPLGCGDLGDDCCDAADLMEEP"
     gene            complement(15118..15384)
                     /locus_tag="JNC24_004478"
     CDS             complement(15118..15384)
                     /locus_tag="JNC24_004478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY9161020.1"
                     /translation="MRPSIIFATAEYVKRLREECLRENKPLHRHTRFRRQELAPDEIN
                     PDVLAMGGHIARRCSEQKRVRIPAMKVSEWGHLLRALEIERVCH"
     gene            complement(15477..15911)
                     /locus_tag="JNC24_004479"
     CDS             complement(15477..15911)
                     /locus_tag="JNC24_004479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000218847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="post-segregation killing protein PndC"
                     /protein_id="HAY9161021.1"
                     /translation="MTVSSTISVFCRDGVFRTVYCHLHGEPTWNGRILHTHYATGQQA
                     EALVEHGDIRCLGPRCDKPAGHTLQNPVDGVTAYYGRDSGFRMDSEAREYRSFREAIA
                     TESTEEVRFHYVFIDGYWKVMYRTPEGWKMKALALALRRCPE"
BASE COUNT         3897 a         4548 c         4002 g         3840 t
ORIGIN      
        1 gaaaccaccg aaaccaccga aaccaccgaa accaccgaaa aacagcatcc cgccgacaag
       61 gaaaaagaac aatgaccatc cactacactg caacactgaa taataaacac ctgtccctga
      121 tcgccctgta ccctgacggt aatcgggagg tgattgccat cctgccagtc acagaactgg
      181 gcgcactgat taaccgtaat aacaggctga tgcacctgtc cttcagttcc ccttttcgcg
      241 aaataaccga aacggtgctt gttgataccg ttgatgcaga acgtcccgga catcacatcg
      301 gcagaatgct ctcagcgatt gatgcctgcc tggatgccga atatgccgga aaaggaaaac
      361 atccctcccg gaaaaatggc ttctggcgga cactgatgtg tggctttatc gccggaattg
      421 ctctgccggc gacagttgca ggttaccact ggatgaaggc agagccccct tccacgctgg
      481 cttaccctac atctcaggaa tggagagctg aaccagtaac cgacacagag acaccacgcc
      541 cacatacgga acatcatgtt aacagcatcc cggcccgccc ggctcccaca cagcaggaac
      601 cttcttctgc ccccgccccg acggcagtga cggaagaaca acacgttcag gccgctgagg
      661 cccgccgtaa tctggccaca gtacttaaac gcaacgcaga ccgggggatg ttcaccatta
      721 acctgtccac tggtcatgaa cgcacgctgt acgccttcct tgaccccgca tgcatcaact
      781 gccgtctgct ggaacccgcg cttaagcaac tggcccggga attcagcgtg gtgatttatc
      841 cggtctccgt gattggcggg gagtccagta caaagcggac tgctccgctg ctgtgtgaac
      901 cggactcaca aaaacgggcg accggatggc atcagctgta ttccgctgac agtggtatgg
      961 cccccaccgg cgatgcaccg gccgcacagg atggacagtg tctgaccgcc gcacgtgctg
     1021 ccatcgatgt caataaccag gctttccgcc agttcggttt tggcggaacc ccctgggtgt
     1081 tatcagatac cggctggcac ctgccgtccg gcatcctgaa agacgccact tccgttcgcc
     1141 tcttcctgaa agcaacagat agcgaggagt atcacaatga gccagcacca cgttaatcct
     1201 gacctcatac accgcacggc ctggggaaac ccgttgtgga atgcactgca taacctgaac
     1261 atcactggtc tgtgtctggc cggcagtatc attacggcgc tgatatggcc actggccctt
     1321 cctgtctgcc tgctgttcac cctggtcacc tccgtgatat tcatgctgca acgctggcga
     1381 tgccccctgc gtatgcccat gacactcagc cttgatgacc cctcccagga cagaaaggtc
     1441 aggagaagcc tcttcagctt ctggcccacc ctgtttcagt atgaagcgga tgaaacattc
     1501 cccgcccggg ggatttttta tgtcggttac cgccgtatca acgatatcgg tcgtgagctc
     1561 tggctgagca tggatgacct cacccggcat gtcatgtttt ttgccaccac cggtggcggt
     1621 aaaacagaaa ccacctttgc ctggctgctc aacccgctct gctggggaag aggctttacc
     1681 tttgttgacg gaaaggcaca gaacgatacc acgaggacaa tctggtatct ctcccgacgt
     1741 tttggcaggg aggatgatat cgaagtcatc aacttcatga acggcggaaa atcccgcagt
     1801 gaaatcattc agagcggcga aaaatcccgc ccacagtcaa acacctggaa cccgttcgca
     1861 ttcagcaccg aagccttcac cgccgaaacc atgcagtcca tgctgcccca gaacgttcag
     1921 ggcggtgaat ggcagtcacg ggcgatagcc atgaacaagg ccctggtctt tggcacaaaa
     1981 ttctggtgcg tccgggaagg taaaaccatg tccctgcaga tgttacggga acatatgacg
     2041 ctggaaggga tggcaaaact ctattgccgt ggccttgatg accagtggcc ggaagaagct
     2101 atcgcaccac tgcgaaacta cctgcaggat gtcccgggat tcgatatgtc actggtgaga
     2161 acgccgtcag cctggactga ggagccccgt aaacagcatg cttatctctc cggtcagttc
     2221 tcggaaacgt tcacaacctt tgccgaaacc ttcggggacg tctttgcggc agatgccggc
     2281 gacatcgata ttcgcgacag tattcacagc gaccgcattc tgattgtgat gatccctgca
     2341 ctggacacat cggctcatac cacgtcggca ctcggtcgta tgttcgtcac ccagcagagc
     2401 atgctcctcg cccgtgacct gggctaccgg ctggaaggca cagatgcaca gaccctggaa
     2461 gtcaaaaaat acaaaggcag cttcccctat atctgtatcc ttgacgaagt cggtgcctac
     2521 tacacagacc gcattgctgt ggaggccacc caggtacgtt cactggagtt ttccctgata
     2581 atgacggcgc aggatcagga gcgaattgaa gggcagacct ccgccaccaa caccgccaca
     2641 ctgatgcaga acgccggcac caaattcgcc ggacgcattg tcagtgacga caaaacggca
     2701 agaacggtga aaaatgccgc cggtgaagag gcacgggcca gaatgggcag cctgcagcgc
     2761 cacgatggtg tgatgggtga gtcctgggtg gacggtaacc agatcaccat ccagatggaa
     2821 agcaaaatcg acgtgcagga tttgatcaga ctgaatgctg gtgagttctt taccgttttc
     2881 cagggggatg tggtgccgtc agcctctgtg tatattccgg acagcgagaa aagttgcgac
     2941 agtgatccgg tggtcatcaa ccgctatatt tccgttgaag ccccccggct ggagcgtctc
     3001 cgccgtctcg tccccaggac agtgcagcgc aggctcccca caccggagca tgtcagcagc
     3061 atcatcggtg tactgacagc aaaaccatcc cgtaaacgtc gtaaaaaccg tacagagcct
     3121 taccgtatca ttgatacatt ccagcgccag ttggcaacag cgcagacatc actggatctg
     3181 cttccgcaat atgatacaga tatcgaatcc cgggcaaacg aactgtggaa aaaggctgtg
     3241 cacactatca ataacacaac cagggaagaa cgccgggtat gttatatcac actgaatcgt
     3301 ccggaagagg aacactccgg tcctgaagat ataccttcag tgaaggcgat tctgaatact
     3361 ctgctccccc tggagatgct gttacctgta ccggatctaa ctgcatcccc gccccataaa
     3421 aaaaatgtcg ctcagacacc atcaggtgga aaacaagaga gtcgtaaaaa aagattctga
     3481 taacacctgt atggctgatt ttaataacaa cctttacgcg attattaatc tcctccgctc
     3541 accatagtaa gcggaggatt caggatttca atccatcacc agaggtacaa taaatgtgta
     3601 ttagtagccc ggaaacaaac tcatggtctg ttatttatcg taaaaattcc ggagaagata
     3661 tcaatatcac ttccctgacg tttaaaaata gtcttctggc agcccggaca ctgatggtgc
     3721 ccgaaaacta tatgatatgc atactcagaa acggggagag ggtcagacga tgggacagag
     3781 aaatacttgc aggctcaaac agatggtaca agtgttctcc cgataatttc gaaatactgg
     3841 gtaagctccc cataataaat aaagtcacaa aactgataaa atcataatct acggatttta
     3901 atcagttaag ctaaatcagg atgaacgctt tgattatgaa ttatacatga aaacaaatat
     3961 gggcagcaca aaaaatggtg ccagggttcc ccacgttcgt aatagattca cttctatgga
     4021 tattggtatg aattcccaga tcccactcta ttgtaaacaa gacattttta tcttttatat
     4081 tcaatggctt attttcctgc taattggtaa taccatgaaa aataccatgc tcagaaaagg
     4141 cttaacaata ttttgaaaaa ttgcctactg agcgctgccg cacagctcca taggccgctt
     4201 tcctggcttt gcttccagat gtatgctctt ctgctcctgc agctaatgga tcaccgcaaa
     4261 caggttactc gcctggggat tccctttcga cccgagcatc cgtatgatac tcatgctcga
     4321 ttattattat tatagaagcc cccatgaata aatcgctcat cattttcggc atcgtcaaca
     4381 taacctcgga cagtttctcc gatggaggcc ggtatctggc gccagacgca gccattgcgc
     4441 aggcgcgtaa gctgatggcc gagggggcag atgtgatcga cctcggtccg gcatccagca
     4501 atcccgacgc cgcgcctgtt tcgtccgaca cagaaatcgc gcgtatcgcg ccggtgctgg
     4561 acgcgctcaa ggcagatggc attcccgtct cgctcgacag ttatcaaccc gcgacgcaag
     4621 cctatgcctt gtcgcgtggt gtggcctatc tcaatgatat tcgcggtttt ccagacgctg
     4681 cgttctatcc gcaattggcg aaatcatctg ccaaactcgt cgttatgcat tcggtgcaag
     4741 acgggcaggc agatcggcgc gaggcacccg ctggcgacat catggatcac attgcggcgt
     4801 tctttgacgc gcgcatcgcg gcgctgacgg gtgccggtat caaacgcaac cgccttgtcc
     4861 ttgatcccgg catggggttt tttctggggg ctgctcccga aacctcgctc tcggtgctgg
     4921 cgcggttcga tgaattgcgg ctgcgcttcg atttgccggt gcttctgtct gtttcgcgca
     4981 aatcctttct gcgcgcgctc acaggccgtg gtccggggga tgtcggggcc gcgacactcg
     5041 ctgcagagct tgccgccgcc gcaggtggag ctgacttcat ccgcacacac gagccgcgcc
     5101 ccttgcgcga cgggctggcg gtattggcgg cgctgaaaga aaccgcaaga attcgttaac
     5161 tgcacattcg ggatatttct ctatattcgc gcttcatcag aaaactgaag gaacctccat
     5221 tgaatcgaac taatattttt tttggtgaat cgcattctga ctggttgcct gtcagaggcg
     5281 gagaatctgg tgattttgtt tttcgacgtg gtgacgggca tgccttcgcg aaaatcgcac
     5341 ctgcttcccg ccgcggtgag ctcgctggag agcgtgaccg cctcatttgg ctcaaaggtc
     5401 gaggtgtggc ttgccccgag gtcatcaact ggcaggagga acaggagggt gcatgcttgg
     5461 tgataacggc aattccggga gtaccggcgg ctgatctgtc tggagcggat ttgctcaaag
     5521 cgtggccgtc aatggggcag caacttggcg ctgttcacag cctatcggtt gatcaatgtc
     5581 cgtttgagcg caggctgtcg cgaatgttcg gacgcgccgt tgatgtggtg tcccgcaatg
     5641 ccgtcaatcc cgacttctta ccggacgagg acaagagtac gccgcagctc gatcttttgg
     5701 ctcgtgtcga acgagagcta ccggtgcggc tcgaccaaga gcgcaccgat atggttgttt
     5761 gccatggtga tccctgcatg ccgaacttca tggtggaccc taaaactctt caatgcacgg
     5821 gtctgatcga ccttgggcgg ctcggaacag cagatcgcta tgccgatttg gcactcatga
     5881 ttgctaacgc cgaagagaac tgggcagcgc cagatgaagc agagcgcgcc ttcgctgtcc
     5941 tattcaatgt attggggatc gaagcccccg accgcgaacg ccttgccttc tatctgcgat
     6001 tggaccctct gacttggggt tgatgttcat gccgcctgtt tttcctgctc attggcacgt
     6061 ttcgcaacct gttctcattg cggacacctt ttccagcctc gtttggaaag tttcattgcc
     6121 agacgggact cctgcaatcg tcaagggatt gaaacctata gaagacattg ctgatgaact
     6181 gcgcggggcc gactatctgg tatggcgcaa tgggagggga gcagtccggt tgctcggtcg
     6241 tgagaacaat ctgatgttgc tcgaatatgc cggggagcga atgctctctc acatcgttgc
     6301 cgagcacggc gactaccagg cgaccgaaat tgcagcggaa ctaatggcga agctgtatgc
     6361 cgcatctgag gaacccctgc cttctgccct tctcccgatc cgggatcgct ttgcagcttt
     6421 gtttcagcgg gcgcgcgatg atcaaaacgc aggttgtcaa actgactacg tccacgcggc
     6481 gattatagcc gatcaaatga tgagcaatgc ctcggaactg cgtgggctac atggcgatct
     6541 gcatcatgaa aacatcatgt tctccagtcg cggctggctg gtgatagatc ccgtcggtct
     6601 ggtcggtgaa gtgggctttg gcgccgccaa tatgttctac gatccggctg acagagacga
     6661 cctttgtctc gatcctagac gcattgcaca gatggcggac gcattctctc gtgcgctgga
     6721 cgtcgatccg cgtcgcctgc tcgaccaggc gtacgcttat gggtgccttt ccgcagcttg
     6781 gaacgcggat ggagaagagg agcaacgcga tctagctatc gcggccgcga tcaagcaggt
     6841 gcgacagacg tcatactaga tatcaagcga cttctcctat cccctgggaa cacatcaatc
     6901 tcaccggaga atatcgctgg ccaaagcctt agcgtaggat tccgcccctt cccgcaaacg
     6961 accccaaaca ggaaacgcag ctgaaacggg aagctcaaca cccactgacg catgggttgt
     7021 tcaggcagta cttcatcaac cagcaaggcg gcactttcgg ccatccgccg cgccccacag
     7081 ctcgggcaga aaccgcgacg cttacagctg aaagcgacca ggtgctcggc gtggcaagac
     7141 tcgcagcgaa cccgtagaaa gccatgctcc agccgcccgc attggagaaa ttcttcaaat
     7201 tcccgttgca catagcccgg caattccttt tcctgccaga catgctgatt aattttgccc
     7261 ttattaacat cacagacaga aagaacgagg gtaccaatac cattgatggt aactggcagg
     7321 ccgatgaagg acgccgttac cgggataatg taagaatcta tttttgagta tgtcaacata
     7381 acctgaggca aagtacctca ggttccagtg tagtaatcag ccaacctgac agtcttctgt
     7441 tagccctaca gatatgtcgt ggagctaacc gcgcatgtca aagccggatt tttgaaaatc
     7501 tctataaact tccggattgt tgaaggccgg gaatttagct ttatgggctg ctgactttat
     7561 cgcttcgcaa taggctttat cgccattact ggttgatatt tttaacgccg tgccatcccg
     7621 ggagaattcc atatgcaacc tgcatttttt ccttttccat ttttgcggat catcaagttt
     7681 agcattaatt gcatccctga ttccccgtgc ttgctccccc cattcatcct gatcatccca
     7741 acgtcctgaa ctgcaactac ccagggctgt agttttatgg caatttgaag gatgtaatgg
     7801 cgcacatccc gccacaaagc tgactaaaat agccagcata acgattttct ttacccccat
     7861 atattgctcc tcaatccata tagatctcag taccatagct ctgtcagagc aaagaaaatc
     7921 ttaactaata caagttaaga tttttaatat ccactcctca tccctacatt ccaggtgatg
     7981 gtggcgtcca ctcattatcc tgaactggag cataagatgc gccatgcccc ccggatatgc
     8041 ttttctgttg cctgatatat tcgtggtagt actgttcact acgctgacga tgtaaatcca
     8101 catccgggcg gctgattaca aagtgtccac ttccggttat tccagctgca ttatgccagg
     8161 ccaccatttc cgcaaataca tcattaaact gttgatatga gccttccggt tcaaaaccca
     8221 ttcttggctc acgatcaaac aatacaggag caatcagatt cagcttatcc accagttcat
     8281 ttctgtcctg atgatggtag aacaccacac ggtccccata atccacacag actaactcgc
     8341 cattcctccg atcacgaaaa cgcacactgc catctttctg cagacgggct tcgagaacgc
     8401 cagtgtcttc ataaagtgga gttcctcccg gttcacagag aatcacgcag cggtcggcat
     8461 tcgttgttcg tcgcttgtct ttgcgacgat cacgataatc ccagccacgt aactgtgata
     8521 atgcagctct gtctccctgt accgcctgtt gctccaccca ctgccggtag gacaaaggat
     8581 accacttacc ctccgcaatc agtgacaacc gctcttcctt cacactctcc ttcagcctga
     8641 tcagcgcctg catacgctgc acttctgcaa tatggtaatg cagcttacgc aattgtggat
     8701 cccggaactg cacacggata tacgccttgc gccgacggca ggcagcatga atttcccgta
     8761 accgttctcc gtaacgtaag tcgggtttac gccagtgctc cttccatgca agataacgcg
     8821 cgcgcaaatc ttcccgcgca gctgcacggg cttcacggcg ttcacgacgc agtacaggat
     8881 cgcgccggaa gccgagctcc acttcatcgc tggcagcaag ttccggattg tagcgacatt
     8941 caggcttcac ccgttcaaat atatctgcac ctgcaatctc aaatggccct gcctgcggct
     9001 cagccctgga taacgtcaaa tccgggtgga tactgctggc cttcactggt gtgagctcat
     9061 gattaaacgc atccacaatc acaagaccat gatgctgttt ctgcaacata agccctttac
     9121 gcgcaaacat ctcatggcac gcctgccagc tgaaattccc gcccgcgatc atatcttcaa
     9181 tcaacgactc acactgaaca gtcgtgtacc cccacagact ttccttatcg gaaaacagtt
     9241 cgcgctttat tgctccctgt ggacctgtcc ccatccgaat acgataccat tcgtaatcac
     9301 tgacagcctg agtaccaaga ttacgtcccc ggaatcgttc tccgggtata acctgagtaa
     9361 aaatatcctt cggtacaggc tgccatccac catccagatc ctgcatataa tccagcgtca
     9421 ttaacggcca cacactgtct gcacgtactg gtgtctgttg tttatcaaat ccatcatgca
     9481 gtaccagatg cccgtgctgg atccccatcc ataatccagc ctttgccaga gttctgtgca
     9541 catccaggca actttccagc tcaccatttc tcgccatttc cggtaagcgt tcggcaaaat
     9601 gacggcaggc gtactgtttc agactctccg tttctgccac tttttccggc cggatatcaa
     9661 cgtttatggc ttccggatca taacgaccgg gtgtccccac ctgactgaaa atatctgtcg
     9721 gtacaggctg atattccccc agtttccgcc ccagtttttc agctgtccat gacggtccga
     9781 acgaactgag tgcaacacct tcccgcgctc tgtcccagcc atccttcacc accagttccc
     9841 cttcctgcat cgtgatatac agaccatcac tggccagccg tttatgcaaa gacaaccagt
     9901 cttgtgcggg ctcttcacgc agcccggcaa tcgacatctg cgcaatatat tccctgaacg
     9961 tctgcttttt cccgcgtcgc caggcattac ggcgctcacg taccagcgct gttttgcgca
    10021 caatacggtt gccgggtgca tgcacaaagc agccattatc aggcgcgaat ccgtgcttaa
    10081 gttcaagttc acgacatgca cggctgagtt tttcctggct ccaggaaagc cagttaatat
    10141 aaccagtttc aggatgtaca cgattcacgg caacatgcac atgcagatta tccgtgtccg
    10201 tatgaacggc agccacgtac tgatgttttg agagttcaag agatttcagt gtgtgacgca
    10261 cacagtcaaa aagctgctca ggacgcgggc tttcatgcgc aggccaggaa agaatgtagt
    10321 ggaaaacggg gtctgtatca ttacgggaaa aaacagcttt gtccgccttg tactgcatct
    10381 cttcagcagc tgtttccagt gaattgcagt tatggaaaca ggttacgcca tagaaattta
    10441 cccactgact gccgtccttc atcacatcaa tcagggagac aaatttttcg ttacgcaggc
    10501 gtgttgcata atcaacaagc cgtctgaaac ggttgcggtg agggacatca ctcttgattt
    10561 cgctgtcttc tgtgagctca ttttcctgca catcatcccg cacagacgtg tacgcaatca
    10621 ggtcttcaaa ggaagacctg ccgtcacgtc ttttgtgcgg aataatggca ttcatcagca
    10681 gttatctcag cgtcccatta agtctcttcg cagagcattg acatattcag taatggcaac
    10741 aagaacctct gcatactcct tatcaccagt gcgttttccc tccacaaaca gatgcttctg
    10801 aaggcggcca agacgcataa tctcatcaat catacggtcg tcagtacggc agttaaccac
    10861 cttatccagt gaagaacttc gtataaatgt tgaaaccgtc tgtccacttg actccgcttt
    10921 ttctcttacg atttcagcct catcctccgt gaatctcgcc gtgaatttta caattctctt
    10981 cctgttctca ctgcccgttc gggttttctt ctcactcatg gtctgctcct taccggggtg
    11041 tcggggcaaa gccctgacca ggtggggaat gtctgagtgc gcgtgcgcgg tccgacattc
    11101 ccacatcctg tcccgttttt cagtccatta tagcctgtta tttttgtgca tcatagtctt
    11161 atgcagacgg cagaggcagg gcgcagccct gccaggagtc agtttttgat gataaaatca
    11221 ggcttcaaca ttaaacggag agaaagtgcc aatggcgtct aaaaaatttt actcagatga
    11281 tgacatccag ctggcaaaag ctgctttatc ggaattgcca gacctcaccg ctcaacggaa
    11341 aacattgcgt gattttcttg atgccattcg tgatgacatc attatcctgg taaggacaaa
    11401 gggttataca ctgacagatg ttcgtgacac gctcaaaaat gcaggatatg aagcaggaga
    11461 gaaagcactt cgtgacatta ttcgggaagc agaatcgaaa aaaccatccc gccgttcttc
    11521 ctctaaaaca gcacctaaaa agacagactc aggcaggaaa gatggcatcg atatgaacaa
    11581 caattgatgt gacaatgtca cattgtgttc tgagattatg atgattacag aaaaaaaagc
    11641 cccggcataa atacctgggc ttttctgttt aatcagactc gtggcggcaa tgcaccagga
    11701 taatgccgtg caataatatc attcagtttt tcaacaaggt cagaacgctt aaagacaatg
    11761 tgtacggtcc ctttctggta gccacgcacc gtaaaatatt ccccttctaa cagcttaccg
    11821 acattgtcac cgttcccgag aaattcacca tactgcgccc cgatgctgtt cctgaagtca
    11881 ggaacgttgc gcccctccag cagataaaac ggacgtacta ggtcatccag tgcctcccgt
    11941 ccgctgcagt ccagcgtcac acgcccgtta ctccagcggt acagtaggtt ttcaataatt
    12001 attttcttac ccagacggca gggattattg gttctgtaat cccatgacaa tttccggtag
    12061 acatcaatca gcccctgttc aaacgtctgc atcttgctgg cattaagatg gcggaatgtt
    12121 gccagtacat tgtccagggt gatttcagga caggtgtcgc tcatcagctg gctgttccac
    12181 tcatcacgct ggcatgaact catgaaggtg tacatgcccg tatctgtcat caggcggtcc
    12241 cagattttcc ggtcggcaag acgggtgata ttccttttcg cttcttccgt cttatccatg
    12301 cgacgaacgg aatcagtcac gcagtcttca aacccgtaaa gaaaaatatc acctgacgca
    12361 tcgagcagaa tcttgcgcgc tgtcgccagg cattccatcg cctgcatgaa cagtgcaata
    12421 ccttcgttac gctgcgcgat gatgcggtcg atagctacag acggaataac atctgtcgtg
    12481 ccggcagtat tcaggtcagc agtgggtaaa gtctgattca tcacattgtc tcctcatcgt
    12541 cacgactgcc gggtcagcca ggtcatttcc cggaggagcg aagctccttc tgccgtccgg
    12601 gtcttcgggg atttccccgc agtggacctt tccgacgggg gacgctcacc acgtcacggg
    12661 gcagttgtcc gggggcagac gcaaagtttt tgacgcctgc ggcaaaaagt tttgtggctg
    12721 gtacatctct cccctggacg actgaaacgg gatgtggtca tgtatgtacc cccggaaagt
    12781 ccgtctgcgg agaacaggcc gcagtgcggc ggcagacagg cagagacagc tgcgccatca
    12841 ggcgcagtgg ccttacacac cgtaaggtgt gcagggagcg ccgcatacgg cgcgactgaa
    12901 gaaaaacgac ggcacaacaa tggcatgtgg tggtcactgg cacaccactc ccgcacaggt
    12961 gaaaacagag aaaaccgcac gacatttaca accaaaaaaa agtgaaattt cttcactttt
    13021 tcacgcttat ttttcgcgtt gatttggtga ttatgatgat aaaataaagt gaaatttttt
    13081 cactttttca aaggtgtttt atgaccggct gggaactgcg aatctggcga aaaagcatgc
    13141 tctggtcacg cgaaaaagcg gccagagaat ttggcgtgac gcagagaaca tggcacgcct
    13201 gggaaaatgc ggaacaggtg gacgtcaccg tctggaggac cacccaggca ctttcagtcc
    13261 gtgacctgct gcctcacatg caggggatgc gtaaggcaga tatcatcagg cgactggaaa
    13321 atgaactggg ggaaacggca gggaatgtgt gagcgtgatt cacagcctgg ctgaatgcca
    13381 ggctactgct catcaggaaa cagctttttc agttcttcac cggaaagtcc cgtgacctcc
    13441 tgaatccgtt ccagcggcca gcctatatcc cgaagccggc aggctatttc ccgcgccact
    13501 tcaagagccc cttgtttaaa accctcgtcg aaacctttct gtataagacg ttctgcaatc
    13561 gtcatgctgt ttgctctgct cctgctgaca aaaaagcccg gcattttgcc gggctttgtg
    13621 gttctcatca gtgagtgatt ttcttcagtt cgtcatcgga aagtccggta gcctgcttaa
    13681 tctgctccgg cgtcatcccc attttctgaa gctggcacgc aatagccagg gctttttcaa
    13741 gcgctccttc catgcgtcct tccatgcgtc cttccatcct gccttcctgg cgaccttttt
    13801 gctcaagacg ttctgcgata gtcatcagtt tatcctcatg ctgcggcaga cgctcagcca
    13861 gcgcccgcat aaattccggt gatacagagt cccctgcgtt aacgatgtaa tgaatcagaa
    13921 cacgcacctg ctcgtctgaa atttccacca tgacctgcgg gagtttgtcc agcagctcca
    13981 tcatgtcacg atggcggata tgcttcatca gcagcgtcag tgcggccatc cggcgatggt
    14041 tcatgatttc attgtcatca acgacagtga tatcaaccag cggaaacggc tttgtgtata
    14101 ttttagccgc aagtgccgga ttctcaaaac agtccagcca gttcatgctg tacgggtacg
    14161 ggctgcgctc accgtgatag aacagcaccg gaatcaccag tggcaacgtt ttgtgctgct
    14221 ccagatgtct ctgcatcgcc gccacactgt agcgtaacat gcgaaaaggc atgtacagat
    14281 tttctgtgct ctggtgctcg atgagcgtat aaacataacc atcttcaccc ccggtggttt
    14341 tcacgctcca gaggatatcg ctggcgtact gatgcaggtc cggctcaaca aaggtggcgg
    14401 gttccagctt cagcgtggac aggtcgcaca actgccggta ctccgcagga agatgcagtt
    14461 ccagaaaatc cctggcaacg tcgggattcg ccaggaacga ccggaacgcg gcatcatgcg
    14521 gcgtgggcgt tgtggtgttt ttcttcttac tcacggttcc tccatcaaat cagcagcatc
    14581 gcagcaatca tcgcccaaat ccccacaacc gagaggacag caattgcgtt caaagccatg
    14641 acaacaatag ccgcgttctg agtcacggcg tatttcttca gcaatctctt ctgcggaaag
    14701 gtaatcaaac tcctcacggg ccagttccag gaccagtaga tgttgttctg tcggcaatgt
    14761 ggccacaaaa ttatcaatag cagcactcaa acgtcgctga tatagctcat aaacaagacc
    14821 acaacggggc attagttctt cggataatag ttccagtttt gagcgaattc tgtcagcgtt
    14881 gaaagtgact tcatcaaaag acatatgaaa agctttgtca gtatcaaaac aagacttcat
    14941 tgttatctcc agccctcccg ggccctgaat gaaagagaat ctgtttccct tttctgtttt
    15001 tttgtggaga caccatgcaa cacagacgtc gtgatgtttt tcatcttgct cacggttcct
    15061 cctggtaaag tacctgaaca catcctgcca cctgcacagg cagacggcag gggttaatca
    15121 gtgacagacc cgttcaattt caagcgcgcg gagcaggtgg ccccattcgc tgactttcat
    15181 agcggggata cgtacccgct tctgctcact gcagcgtctg gcgatatgac cgcccatcgc
    15241 caggacgtcc gggttaatct catccggtgc cagttcctgg cgtctgaagc gggtatggcg
    15301 gtgcaggggt ttgttctccc gcaggcactc ttcacgcaga cgctttacat actcggcggt
    15361 tgcgaagata attgatggtc tcatggcata atctccatag ttagtgaatg agatgccgga
    15421 cttccgtacg gggaagcgcc agcggagaaa ggcagggttt atcgccctgc tttttttcat
    15481 tccggacagc gacgcagtgc cagcgcgagc gctttcatct tccagccttc cggcgtgcgg
    15541 tacatcacct tccagtagcc gtcgatgaac acataatgga agcgcacctc ttcagtgctt
    15601 tcagtggcaa tagcctccct gaaagaacgg tactcacgcg cctcactgtc catccggaaa
    15661 ccactgtcac gtccgtaata agccgtcaca ccgtccaccg ggttctgaag cgtatgcccg
    15721 gcgggtttgt cgcaacgggg accgaggcaa cggatatcac cgtgttcaac cagggcttcg
    15781 gcctgctgac cggtggcata gtgggtatgc aggatgcgac cgttccaggt cggctcaccg
    15841 tgcaggtggc agtaaacggt acggaacacc ccgtcgcggc aaaaaacgga aatcgtgctg
    15901 ctgactgtca tgaaaacgtc tccttctgct gaatcaggtc gccgggccga ccggttgctc
    15961 ccccggaggg gcgaaagccc cttctgccgt ccacttcttc ggggtttctc cccgcagtgg
    16021 acctttccga tggggcagcg acagccacgg cacggggcag ttgtccgggg gcagacgcaa
    16081 aagtttttga cgcttgcggc aaaaagtttt gtggctggta catctccccc ctggacggct
    16141 gacacgtgtt gtggccgctt cagcccccgg aaagtccaca gcgggaaaca ggccgcaggg
    16201 gtggcggcgg aagggcagac aactgcgcca tcaggcgcag tttccttaca caccgtcagg
    16261 tgtgcaggga gcgcaggtca cggcgcg
//
LOCUS       DADMKF010000446         9932 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.guided.140, whole genome shotgun sequence.
ACCESSION   DADMKF010000446 DADMKF010000000
VERSION     DADMKF010000446.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9932
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.guided.140"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            complement(16..204)
                     /locus_tag="JNC24_004480"
     CDS             complement(16..204)
                     /locus_tag="JNC24_004480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HAY9161022.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMVMLPTY"
     gene            complement(423..1400)
                     /gene="epmA"
                     /locus_tag="JNC24_004481"
     CDS             complement(423..1400)
                     /gene="epmA"
                     /locus_tag="JNC24_004481"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HAY9161023.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            1725..3533
                     /gene="frdA"
                     /locus_tag="JNC24_004482"
     CDS             1725..3533
                     /gene="frdA"
                     /locus_tag="JNC24_004482"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HAY9161024.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQECFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            3526..4260
                     /locus_tag="JNC24_004483"
     CDS             3526..4260
                     /locus_tag="JNC24_004483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HAY9161025.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALVYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            4271..4666
                     /gene="frdC"
                     /locus_tag="JNC24_004484"
     CDS             4271..4666
                     /gene="frdC"
                     /locus_tag="JNC24_004484"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HAY9161026.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            4677..5036
                     /gene="frdD"
                     /locus_tag="JNC24_004485"
     CDS             4677..5036
                     /gene="frdD"
                     /locus_tag="JNC24_004485"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HAY9161027.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
     gene            5099..6232
                     /gene="blaEC"
                     /locus_tag="JNC24_004486"
     CDS             5099..6232
                     /gene="blaEC"
                     /locus_tag="JNC24_004486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HAY9161028.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLTPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            6321..6854
                     /locus_tag="JNC24_004487"
     CDS             6321..6854
                     /locus_tag="JNC24_004487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HAY9161029.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDREMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(6851..7168)
                     /locus_tag="JNC24_004488"
     CDS             complement(6851..7168)
                     /locus_tag="JNC24_004488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound-resistance protein
                     SugE"
                     /protein_id="HAY9161030.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(7343..7489)
                     /gene="ecnB"
                     /locus_tag="JNC24_004489"
     CDS             complement(7343..7489)
                     /gene="ecnB"
                     /locus_tag="JNC24_004489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HAY9161031.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(7600..7725)
                     /gene="ecnA"
                     /locus_tag="JNC24_004490"
     CDS             complement(7600..7725)
                     /gene="ecnA"
                     /locus_tag="JNC24_004490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HAY9161032.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(7777..8343)
                     /gene="efp"
                     /locus_tag="JNC24_004491"
     CDS             complement(7777..8343)
                     /gene="efp"
                     /locus_tag="JNC24_004491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HAY9161033.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            8385..9413
                     /gene="epmB"
                     /locus_tag="JNC24_004492"
     CDS             8385..9413
                     /gene="epmB"
                     /locus_tag="JNC24_004492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HAY9161034.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPGELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPILRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
     gene            9803..9898
                     /locus_tag="JNC24_004493"
                     /pseudo
     CDS             9803..9898
                     /locus_tag="JNC24_004493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313153.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         2544 a         2519 c         2514 g         2355 t
ORIGIN      
        1 ccatcataca ctaaatcagt aagttggcag catcaccatt aaggcgaatg gaataaagaa
       61 taacaatgca gaaaatatat aaaagggaat cgcactataa cccattaagt aataagccga
      121 tgggctattg gcaaatccaa atacggaagt aaagatcatc aatatgagtc ctatcagact
      181 catctttttt atcgtgtgag gcatgaaacc atccttcatt tcagcgcgtg tataacacgt
      241 gcccactgaa ctgcactgcg caacattgag caggcagcgt aaaaaccaat acagaattga
      301 tacccggatg atatcaaata tccagtctta aattgtggcc tttaacggaa aaatgaaaac
      361 atatgctaca gaatggcgct tatcacgcca ttcttcgctg ttaattcagt aatttttcag
      421 aattatgccc ggtcaacgct aaaggcgatg acttcagcca gtgtctccgc gcccagcgcc
      481 aacatcacca gacgatcaac acctaatgcc acgccggaac agtcaggcat accgactttc
      541 aaggcttcaa tcagattctg gtcaatgggg tgctgcggca aaccgcgcgc cgcgcgctta
      601 cggttatctt gttcaaagcg ttgttgctgc tcacgggcat ccgttaattc atggaaacca
      661 ttcgccagct caataccttt ataataaacc tcaaagcgtt cagcgacccg atgatcttcg
      721 gtactgattt gcgccagtga tgcctggctg gctggaaagt ggtacacaaa ggtcggtttt
      781 tctttgccaa tatttggctc tacgccaaag gtaaacagca attgtagcaa cgtgtcgcgg
      841 tcttcttcgg tatcagcaac gttactcaaa tccagtttcg ctgcgacttc ccgcagttgc
      901 gttttgtcgg cagagagcgg gtcaatttcc agataacgca agaaagcttg ttgataagaa
      961 aggctttctg ctgccgggca gtccagcacc tgttgtaaga gatcgtccac ctcgttcatc
     1021 aaccggtaca tatcatagtg cggtcgatac cactcaagca tagtgaactc agggttgtga
     1081 taacgcccca tctcttcatt acggaagctg cggcacagct ggaataccgg cccacaaccg
     1141 gcaaccagca ggcgtttcat atggtattcc gggctggtca ttaaccagag attcatcccc
     1201 tgcgaatgcc cggggccaac gaaacgtgtc tcaaacggga ccaaatgaat atcggttacc
     1261 gtcgcctggc tcatacaagg cgtctccacc tccagcactc cacgatcggc aaagaaacga
     1321 cggatctccg ccataatcgc cgcacgtttt aataagttag gaatggatgc gctcggctgc
     1381 caggatgccg tttcgctcat agttaaatct ccagtttttg acaagggcac gaagtctact
     1441 cgcaacgcga cggcgagaca aattttacgc aggaatcaaa cagcggtggg cagtgactaa
     1501 aaaaagcacg atctgatggt ttagtaatta aattaatcat cttcagtgat aatttagccc
     1561 tcttgcgcac taaaaaaatc gatctcgtca aatttcagac ttatccatca gactatactg
     1621 ttgtacctat aaaggagcag tggaatagcg ttcgcagacc gtaactttca ggcacttacc
     1681 ctgaagtacg gggctgtggg ataaaaacaa tctggaggaa tgtcgtgcaa acctttcaag
     1741 ccgatcttgc cattgtaggc gccggtggcg cgggattacg tgctgcaatt gctgccgcgc
     1801 aggcaaatcc aaatgcaaaa atcgcactaa tctcaaaagt atacccgatg cgtagccata
     1861 ccgttgctgc agaagggggc tccgccgctg tcgcgcagga tcatgacagc ttcgaatatc
     1921 actttcacga tacagtagcg ggtggcgact ggttgtgtga gcaggatgtc gtggattatt
     1981 tcgtccacca ctgcccaacc gaaatgaccc aactggaact gtgggggtgc ccatggagcc
     2041 gtcgcccgga tggtagcgtc aacgtacgtc gcttcggcgg catgaaaatc gagcgcacct
     2101 ggttcgccgc cgataagacc ggcttccata tgctgcacac gctgttccag acctctctgc
     2161 aattcccgca gatccagcgt tttgacgaac atttcgtgct ggatattctg gttgatgatg
     2221 gtcatgttcg cggcctggta gcaatgaaca tgatggaagg cacgctggtg cagatccgtg
     2281 ctaacgcggt cgttatggct actggcggtg cgggtcgcgt ttatcgttac aacaccaacg
     2341 gcggcatcgt taccggtgac ggtatgggta tggcgctaag ccacggcgtt ccgctgcgtg
     2401 acatggaatt cgttcagtat cacccaaccg gtctgccagg ttccggtatc ctgatgaccg
     2461 aaggctgccg tggtgaaggc ggtattctgg tcaacaaaaa tggctaccgt tatctgcaag
     2521 attacggcat gggcccggaa actccgctgg gcgagccgaa aaacaaatat atggaactgg
     2581 gtccacgcga caaagtttct caggccttct ggcacgaatg gcgtaaaggc aacaccatct
     2641 ccacgccgcg tggcgatgtg gtttatctcg acttgcgtca cctcggcgag aaaaaactgc
     2701 atgaacgtct gccgttcatc tgcgaactgg cgaaagcgta cgttggcgtc gatccggtta
     2761 aagaaccgat tccggtacgt ccgaccgcac actacaccat gggcggtatc gaaaccgatc
     2821 agaactgtga aacccgcatt aaaggtctgt tcgccgtggg tgaatgttcc tctgttggtc
     2881 tgcacggtgc aaaccgtctg ggctccaact ccctggcgga actggtggtc ttcggccgtc
     2941 tggccggtga acaagcgaca gagcgtgcag caactgccgg taatggcaac gaagcggcaa
     3001 ttgaagcgca ggcagctggc gttgaacaac gtctgaaaga tctggttaac caggatggcg
     3061 gcgaaaactg ggcgaagatc cgcgacgaaa tgggcctggc aatggaagaa ggttgcggta
     3121 tctaccgtac gccggaactg atgcagaaaa ccatcgacaa gctggcagag ctgcaggaat
     3181 gcttcaagcg cgtgcgcatc accgacacct ccagcgtgtt caacaccgac ctgctctaca
     3241 ccattgaact gggccacggt ctgaacgttg ctgaatgtat ggcgcactcc gcaatggcac
     3301 gtaaagagtc ccgcggcgca caccagcgtc tggacgaagg ttgcaccgag cgtgacgacg
     3361 tcaacttcct caaacacacc ctcgccttcc gcgatgctga tggcacgact cgcctagagt
     3421 acagcgacgt gaagattact acgctgccgc cagctaaacg cgtttacggt ggcgaagcgg
     3481 atgcagccga taaggcggaa gcagccaata agaaggagaa ggcgaatggc tgagatgaaa
     3541 aacctgaaaa ttgaggtggt gcgctataac ccggaagtcg ataccgcacc gcatagcgca
     3601 ttctatgaag tgccttatga cgcaactacc tcattactgg atgcgctggt ctacatcaaa
     3661 gacaacctgg caccggacct gagttaccgc tggtcctgcc gtatggcgat ttgtggttcc
     3721 tgcggcatga tggttaacaa cgtgccaaaa ctggcatgta aaaccttcct gcgtgattac
     3781 accgacggta tgaaggttga agcgttagct aacttcccga ttgaacgcga tctggtggtc
     3841 gatatgaccc acttcatcga aagtctggaa gcgatcaaac cgtacatcat cggcaactcc
     3901 cgcaccgcgg atcagggtac taacatccag accccggcgc agatggcaaa gtatcaccag
     3961 ttctccggtt gcatcaactg tggtctgtgc tacgccgcgt gcccgcagtt tggcctgaac
     4021 ccagagttca tcggtccggc tgccattacg ctggcgcatc gttataacga agatagccgc
     4081 gaccacggta agaaggagcg tatggcgcag ttgaacagcc agaacggcgt atggagctgt
     4141 actttcgtgg gctattgctc cgaagtctgc ccgaaacacg tcgatccggc tgcggccatt
     4201 cagcagggca aagtagaaag ttcgaaagac tttcttatcg cgaccctgaa accacgctaa
     4261 ggagtgcaac atgacgacta aacgtaaacc gtatgtacgg ccaatgacgt ccacctggtg
     4321 gaaaaaattg ccgttttatc gcttttacat gctgcgcgaa ggcacggcgg ttccggctgt
     4381 gtggttcagc attgaactga ttttcgggct gtttgccctg aaaaatggcc cggaagcctg
     4441 ggcgggattc gtcgactttt tacaaaaccc ggttatcgtg atcattaacc tgatcactct
     4501 ggcggcagct ctgctgcaca ccaaaacctg gtttgaactg gcaccgaaag cggccaatat
     4561 cattgtaaaa gacgaaaaaa tgggaccaga gccaattatc aaaagtctct gggcggtaac
     4621 tgtggttgcc accatcgtaa tcctgtttgt tgccctgtac tggtaaggag cctgagatga
     4681 ttaatccaaa tccaaagcgt tctgacgaac cggtattctg gggcctcttc ggggccggtg
     4741 gtatgtggag cgcaattatt gcgccggtga tgatcctgct ggtgggtatt ctgctgccac
     4801 tggggctgtt tccgggtgat gcgctgagct acgagcgcgt tctggcgttc gcgcagagct
     4861 tcattggtcg cgtattcctg ttcctgatga tcgttctgcc gctgtggtgt ggtttacacc
     4921 gtatgcacca cgcgatgcac gatctgaaaa ttcacgtgcc tgcgggcaaa tgggttttct
     4981 acggtctggc tgctatcctg acagttgtca cgctgattgg tatcgttaca atctaacgta
     5041 tcgccaatgt aaatccggcc cgcctatggc gggccgtttt gtatggaaac cagaccttat
     5101 gttcaaaacg acgctctgcg ccttattaat taccgcctct tgctccacat ttgctgcccc
     5161 tcaacaaatc aacgatattg tgcatcgcac aattaccccg cttatagagc aacaaaagat
     5221 ccccggtatg gcggtggcgg taatttatca gggtaaacct tattacttta cctggggcta
     5281 tgcggacatc gccaaaaagc agcccgtcac acagcaaacg ttgtttgagt taggttcggt
     5341 cagcaaaaca tttacgggcg tgcttggtgg cgacgctatt gctcgagggg aaatcaagtt
     5401 aagcgatccc gcaacaaaat actggcctga acttactgct aaacagtgga atgggatcac
     5461 actattacat cttgcgacct acaccgctgg cggcctgcca ttgcaggtgc cggatgaagt
     5521 gaaatcctca agcgacttgc tgcgcttcta tcaaaactgg cagcctgcat gggctccagg
     5581 aacacaacgt ctgtatgcca actccagtat cggtttgttc ggcgcactgg ctgtgaagcc
     5641 gtctggtttg agttttgagc aggcgatgca aactcgtgtc ttccagccac tcaaactcaa
     5701 ccatacgtgg attaatgtac ctcccccaga agaaaagaat tacgcctggg gatatcgcga
     5761 aggtaaggca gtgcatgttt cgccaggggc gttagatgct gaagcttatg gtgtgaagtc
     5821 gaccattgaa gatatggccc gctgggtgca aagcaattta accccccttg atatcaatga
     5881 gaaaacgctt caacaaggga tacaactggc acaatctcgc tactggcaaa ccggcgatat
     5941 gtatcagggc ctgggctggg aaatgctgga ctggccggtc aatcctgaca gcatcattaa
     6001 cggcagtgac aataaaattg cactggcagc acgcccggta aaagcgatta agcccccaac
     6061 tcctgcagta cgcgcatcat gggtacataa aacgggggcg accggcggat ttggtagcta
     6121 tgtcgcgttt attccagaaa aagagctggg tatcgtgatg ctggctaaca aaaactatcc
     6181 caatccagcg agagtcgccg ccgcctgtca gattctcaac gctctacagt aaaatttcat
     6241 cgggtccgaa ttttcggacc ttttctgcgc ttttccttgc tgtcatctac acttagaaaa
     6301 aaaccagtaa ggaaacattt atgcgcctgc tccctctcgt tgccgcagcg acagctgcat
     6361 ttctggtcgt tgcctgcagt tctcctacgc cgccgcgtgg cgtgacagta gtgaataatt
     6421 tcgacgctaa acgctatctc ggtacctggt atgagattgc ccgttttgat caccgttttg
     6481 aacgtggact ggaaaaagtc accgcaacat acagtctgcg tgatgacggc ggcctgaatg
     6541 tcatcaataa aggctataac cctgacagag aaatgtggca gcagagtgaa gggaaagcgt
     6601 actttaccgg cgcaccaaca cgcgctgcgc tgaaagtgtc attctttggt cctttctatg
     6661 gcggttataa cgtcattgca cttgatcggg aataccgcca tgcgctggtt tgcggcccgg
     6721 accgcgacta cctgtggata ctctcccgca cgccaaccat ttctgacgaa gtgaaacagg
     6781 agatgctggc agtcgcgacc cgggaagggt ttgatgtcag taagtttatt tgggtacagc
     6841 agcccggtag ttagtgagtg ctgagtttca gaccaataat ccccaatacg attaacgcca
     6901 gactcgccag gcgcatcggg ttagcggact caccgagcag cacaatgccg gtgatggccg
     6961 cgccgactgc gccaataccc gtccacacgg cataagccgt ccctaccggt aacgatttca
     7021 tcgcccaggc aagtagcgcc atactgacta tcatcgccgt cacagtaata acacttggcg
     7081 tcaaacgact aaagccgtgg gtatatttca ggccaacggc ccataccact tccagcagac
     7141 cagcaataac taagataatc caggacatat caggctccgg agaattgggg ccatccccgg
     7201 tgaaaagagg cgtttgcagg tcgtcctgca aagctgatgg ttgaacggat attttgccag
     7261 gtggcagggg aatttcaact attacctttg tgactatcgc aaaaaaacaa acggcacgac
     7321 ataactgtcg taccgttatt gcttattgct gcgctttcgt tgctgcacca gaaatcgcgt
     7381 taccgccatc agaaatgtct tcaccaacgc cacgcgtggt gttgcaggca gttaatactg
     7441 ttgaaagcac cagaacagaa aagatcgctg caattgtctt cttcaccata acgtcttcct
     7501 tttaggtaat gttatttatg tttgcctata gcaaattaat aatagacaac atcgccaaaa
     7561 atgatggaaa tctgatgatt tttcggaaga gaagaaaaat tagctggcag cgcgggagat
     7621 ggagttgccg agatgtttga tgtcttcgcc gaaaccgcga gcggtgttac agcccgtgag
     7681 cagcgtgctg gcaagcaaaa caagaacgat aaggcgtttc atcatctctg acctgaataa
     7741 gtgatggtgc agcctgcagg ccgcaccaca accgcattac ttcacgcgag agacgtattc
     7801 accagagcgg gtatccactt tgatgacttc gccgatttgt acgaacagcg gaactttaac
     7861 cacagcgcca gtagacaggg tagccggttt gccgccagta cctgcggtat cacctttcag
     7921 gcccggatcg gtatcaacga tttccagttc aacgaagttc ggcggagtaa cggagatcgg
     7981 ctgaccattc cacagagtta cgatacactc tgcctgatcc agcagccatt tagcgttgtc
     8041 gccaattgct tttgcatcag cagacagctg ctcgaaagtt tcgttgttca tgaagtgcca
     8101 gaactcaccg tcgttgtaca ggtaagtcag gttcatatcg acaacatcag cgccttcagc
     8161 ggaatcagta gatttgaagg ttttttctac gcgagtaccg gtcagcagac gacgcagttt
     8221 aacgcgagca aatgcctggc ctttacccgg ttttacgaat tcactcgctt caaccgcgta
     8281 aggttcgccg tctaacatga ttttaagacc agcacgaaaa tcgttgctat agtacgttgc
     8341 cataaggccc tctgaaattt gttaattggt agctaagcca caaaatggcg catattgtaa
     8401 ccctaaatac cccatccaga gaagattggt taacgcaact tgccgatgtt gtgaccgatc
     8461 ctggtgaact tctgcgtctt ttgaatatag acgcggacga aaaactgttg gccggacgca
     8521 gcgccaaaaa gctgtttgcc ctgcgtgtgc cccgctcatt tatcgatcgc atggagaaag
     8581 gcaatccgga cgatcctatt ttgcgtcagg tacttacctc gcaagatgag tttgtcgtcg
     8641 cgtccggatt ctccaccgac ccactggaag aacagcacag cgtagtgcct ggtttgttgc
     8701 ataaatacca caaccgggcg cttttgctgg tcaaaggcgg ctgcgcggta aattgccgct
     8761 attgcttccg tcgccacttc ccctatgccg aaaatcaggg caacaagcgt aactggcaga
     8821 ctgcacttga gtatgttgct gcgcatccgg aactggacga gatgattttc tccggcggcg
     8881 atccgctgat ggcgaaagat cacgagctgg actggttgct cacacaactg gaagccatcc
     8941 cgcatatcaa acgtctgcgg attcacagcc gtctgccgat tgtgatcccg gcacgtatca
     9001 ccgaggcgct ggttgaacgc tttgcccgtt ctacgctgca aatcttgctg gtgaatcaca
     9061 tcaatcatgc caatgaggta gatgaaacgt tccgtcaggc gatggctaaa ttgcgccgtg
     9121 tcggtgtcac cctgcttaac cagagcgttc tgttacgtgg tgtgaacgat aacgcacaaa
     9181 cgttggcaaa cctgagtaat gcgttgttcg atgccggcgt gatgccgtat tacctgcatg
     9241 tgctagataa agtacagggc gcggcgcatt ttatggtgag tgatgacgaa gcacggcaga
     9301 ttatgcgtga gttgctgaca ttggtgtcgg gatatctggt gccgaaactg gcgcgagaaa
     9361 ttggcggcga acccagcaaa acgccgctgg atctccagct acgccagcag taactgatta
     9421 aacagggaag ttaaacatta gcctgatgcg ctacgcttat caggcctaca ttgtgcattg
     9481 aaattgaatg gatttgcaat gaactgtagg ccggataagg cgtttacgcc gcatccggca
     9541 agagcaacaa acgcaagtac agcaacctgt cattaaactg ggaaatttaa tctttgcccg
     9601 ttgcataacc tttaattatt catatacatt gctgttgtta ctctttatat agccaacacc
     9661 aataattaac ctttcttttc tgtttatata gagtgagtac ggctatttat gttattttaa
     9721 caactattac taccaagccc atttatctgg cttataaatt aaaattaatt atttaattga
     9781 gaaatggtta gggagaacct acatggccat aagcatcaag ggtgttaata ccggtgttat
     9841 tcgcaagagt aataatttca ttgcactggc gctgaaaatt aaagaacctc gcaataggta
     9901 atgactccaa cttattgata gtgttttatg tt
//
LOCUS       DADMKF010000447         6715 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.guided.192, whole genome shotgun sequence.
ACCESSION   DADMKF010000447 DADMKF010000000
VERSION     DADMKF010000447.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6715
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.guided.192"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            164..652
                     /locus_tag="JNC24_004494"
                     /pseudo
     CDS             164..652
                     /locus_tag="JNC24_004494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001341229.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rtcB domain protein"
     gene            649..1263
                     /locus_tag="JNC24_004495"
     CDS             649..1263
                     /locus_tag="JNC24_004495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HAY9161035.1"
                     /translation="MILLQLSSAQGPEECCLAVKKALERLIKEAARQDVAVTVLETET
                     GRYSDTLRSALVSLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAVLKSQRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(1320..2777)
                     /gene="pepD"
                     /locus_tag="JNC24_004496"
     CDS             complement(1320..2777)
                     /gene="pepD"
                     /locus_tag="JNC24_004496"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008783788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HAY9161036.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKSVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDVL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            3038..3496
                     /gene="gpt"
                     /locus_tag="JNC24_004497"
     CDS             3038..3496
                     /gene="gpt"
                     /locus_tag="JNC24_004497"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HAY9161037.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            3588..4832
                     /gene="frsA"
                     /locus_tag="JNC24_004498"
     CDS             3588..4832
                     /gene="frsA"
                     /locus_tag="JNC24_004498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HAY9161038.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     IQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            4890..5291
                     /locus_tag="JNC24_004499"
     CDS             4890..5291
                     /locus_tag="JNC24_004499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HAY9161039.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(5330..6385)
                     /gene="phoE"
                     /locus_tag="JNC24_004500"
     CDS             complement(5330..6385)
                     /gene="phoE"
                     /locus_tag="JNC24_004500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HAY9161040.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAVGMTYQF"
BASE COUNT         1658 a         1637 c         1708 g         1712 t
ORIGIN      
        1 acaccccatt ataaggggta cgaataaagt aacggggcag tctattttac agcggcatat
       61 tgcctcgttt tcgcatcatg gtttgcctga aggcagtgac gacgcgctac ggtatattgc
      121 ggaacatgac gatgcgctgg cgtttgcgcg tattaatcgc cagctgatct ctttacgcat
      181 aatgcaacag gttaaggcca ccggaagtcc ggttctggat gtggcgcata actttgttag
      241 cgcgtgtcaa atcggcgatc aacagggctg gttgcatcgt aaaggtgcca cgccggatga
      301 caacggtctg gtgattattc ccggttcact cggtgattac tcctagcttg tacagcctgt
      361 tgcgaacgaa aaaacgctcc actcgctggc gcatggggct gggcgtaaat gggggcgcac
      421 cgagtgtaaa gggcgtctgg cagcgaaata cacagcgacg cagctctcac gtactgaact
      481 tggcagtcgg gtaatttgtc gcgataaaca actcatcttt gaagaagcgc cacaagccta
      541 taaatcggct gaaagcgtgg tgcaatgtct ggtgcaggct ggtttaatta ttcctgtcgc
      601 gcggctgcgt ccggtgctaa cgctcaaaaa cagtggaggg aaaaaaggat gatcttgcta
      661 caactctcct cagcgcaggg gccggaagaa tgttgtctcg cagtgaaaaa ggcgctggag
      721 cgattaatta aagaagctgc ccgacaagac gtcgcggtaa cggtgttgga aacagaaacg
      781 ggccgctact ctgacacgct gcgttcagct ctggtttctc tggatggcga taacgcatgg
      841 gcattaagcg aaagctggtg cggcactatt cagtggattt gtccgagtcc gtatcggcct
      901 catcatgggc gcaaaaactg gtttctgggc attgggcgtt ttaccgctga tgagcaggaa
      961 caatcggatg caatccgtta tgagacgttg cgttcgtcgg ggccgggcgg tcaacatgtc
     1021 aataaaaccg actcggcggt acgcgccacg catttggcat ccggtattag cgtgaaggtt
     1081 cagtcagagc gtagtcagca tgctaacaag cggctggcac gattgctgat tgcctggaag
     1141 ctggagcagc agcaacagga aaatagcgcg gtgctgaaat cgcagcggcg aatgttccat
     1201 caccagattg aacgtggcaa cccgagacgg acatttacag ggatggcttt tatcgaagga
     1261 taatgaagga tgaaactgcc ggaaggcgat taaacgccat ccggcagcaa atcaaataat
     1321 tacttcgccg gaatttcttt cagcagttca gtcagcagtg tccagtaatg acctacgctt
     1381 tcgatgtgaa cttgctcatc cggagagtgt ggaccggtga tagttggccc gatagaaacc
     1441 atgtccattt ccggatacgg ttttttgaac agaccacatt ccaggcccgc gtggataatc
     1501 tggatgttcg gcgttttgtt gaacagacgc tgataggttt cacgtaccag atgcatcacc
     1561 ggagaattag cgtccggctg ccagccagga tatgcgcctt tcgcttcggt tttcgcgcca
     1621 gccagtttac ccagcgaatc cagcatgctc accacgtagt ctttaccgct gtcgatcaga
     1681 gaacggatca gacagtgaat ttctacgtta ttatcagtca tggtcaccac accgacgttc
     1741 agggaggttt cgaccacacc ttttgccacg tctgagttgc ggatcacacc gttcggggtg
     1801 gcgttcagca gacgaataaa ggtatcgcga gatttcgcaa tcagggcagc tttatcgttc
     1861 gctacagagt ccagcaacaa ggccagattt ttctctttct ctgccagctc gtttttcagg
     1921 atctcctgat aggtattcac cagagatttc aggacgtcga ctttatcagc tgcgacagca
     1981 atggtcgcaa aggcttcacg cgggatggcg ttacgcagtg tgccgccgtt gaaatcgata
     2041 aggcgcagat ccagttcttc tgcatgaccc gctaggaagc gcaccagcag tttgttggca
     2101 ttacccaggc caacgtggat ttccccgccg gagtgacccc ctttcagacc ttttaaggtt
     2161 aacttgaagg tttcaaaacc agctggaacc gcttcacgat ctaaatgcag gttggaggtg
     2221 aagtcgatac cccccgcaca acccatgtag atttcacctt cttcttcgga gtcggtgtta
     2281 atcagaatat cagcctgcaa ccagttgctc tgtaagccga acgcaccgtc cataccggct
     2341 tcttcggtca tggtcagcag cacttccagc gggccgtgaa ccacgttttc gtcagccaga
     2401 accgccagcg cagacgccat accaatgccg ttatctgcac ccagcgtggt gccgcgcgct
     2461 ttaacccatt cgccatcaat ataaggctgg ataggatctt tcgtgaagtc atgcacggtg
     2521 tcgttatttt tctgcggcac catatcgagg tgggcctgta agacgaccga tttacgattt
     2581 tccatacctg cggtagcagg tttacgaatc aggatattac ctacctgatc gcgttcgaca
     2641 tggaaacctt tctcttttgc ccaaccaaca atgtattcag cgagttgctc ttcatgatag
     2701 gacgggtgag gaatagaaca gattttggca aaaatatccc acagcggctg tggagataat
     2761 tgagacagtt cagacacgtt aagtctcctt gtcgatcacc cgcaaaacag tattgcaggt
     2821 cacagggtta gcagaaaatg ttgtcaacac aagacaggct tgcgagatat gtttgagaat
     2881 accactttat cccgcgtcag ggagaggcag tgcgtaaaaa gacgcggact catgtgaaat
     2941 actggttttt agtgcgccag atctctataa tctcgcgcaa cctattttcc cctcgaacac
     3001 tttttaagcc gtagataaac aggctgggac acttcacatg agcgaaaaat acatcgtcac
     3061 ctgggacatg ttgcagatcc atgcacgtaa actcgcaagc cgactgatgc cttctgaaca
     3121 atggaaaggc attattgccg taagccgtgg cggtctggta ccgggtgcgt tactggcgcg
     3181 tgaactgggt attcgtcatg tcgataccgt ttgtatttcc agctacgatc acgacaacca
     3241 gcgcgagctt aaagtgctaa aacgcgcaga aggcgatggc gaaggcttca tcgttattga
     3301 tgacctggtg gataccggtg gtactgcggt tgcgattcgt gaaatgtatc caaaagcgca
     3361 ctttgtcacc atcttcgcaa aaccggctgg tcgtccgctg gttgatgact atgttgttga
     3421 tatcccgcaa gatacctgga ttgaacagcc gtgggatatg ggcgtcgtat tcgtcccgcc
     3481 aatctccggt cgctaatctt ttcaacgcct ggcactgccg ggcgttgttc tttttaactt
     3541 caggcgggtt acaatagttt ccagtaagta ttctggaggc tgcatccatg acacaggcaa
     3601 acctgagcga aaccctgttc aaaccccgct ttaaacatcc tgaaacctcg acgctagtcc
     3661 gccgctttaa tcacggcgca caaccgcctg tgcagtcggc ccttgatggt aaaaccatcc
     3721 ctcactggta tcgcatgatt aaccgtctga tgtggatctg gcgcggcatt gacccacgtg
     3781 aaatcctcga cgtccaggcg cgtattgtga tgagcgatgc cgaacgtacc gacgatgatt
     3841 tatacgatac ggtgattggc taccgtggtg gcaactggat ttatgagtgg gccatccagg
     3901 cgatggtgtg gcaacaaaaa gcctgtgcgg aagaagatcc gcaactcagt ggtcgtcact
     3961 ggctgcatgc ggctacgttg tacaacattg ctgcctatcc tcatctgaaa ggagatgacc
     4021 tggccgagca agcgcaggct ttgtcaaacc gtgcctatga agaagccgcc cagcgtctgc
     4081 cgggcacgat gcggcagatg gagtttaccg tacccggcgg tgcgcccatc accggctttt
     4141 tgcatatgcc gaaaggcgat ggcccgttcc cgacagtatt aatgtgtggt ggtctggatg
     4201 cgatgcagac ggactattac agcctgtatg aacgttattt tgcgccgcgc ggcattgcga
     4261 tgctgactat tgatatgccg tcggtgggct tttcatcaaa atggaagctc acccaggact
     4321 ccagcctgtt gcatcagcac gttttaaagg ctctgcctaa cgtaccgtgg gtggatcaca
     4381 ctcgcgtcgc ggcctttggt ttccgtttcg gcgctaacgt tgccgtgcgt ctggcatatc
     4441 ttgaatcgcc gcgtctgaaa gcggttgcct gtcttggtcc ggtagttcat accctgttga
     4501 gtgattttaa gtgccagcaa caggtgccgg aaatgtatct tgacgttctg gcgagtcgtt
     4561 tggggatgca tgatgcttcc gacgaagcgt tacgcgtgga gctgaatcgc tattcattaa
     4621 aagtgcaagg attgctgggg cgtcgctgcc caacgccaat gttatcaggc tactggaaga
     4681 acgatccgtt cagcccggaa gaggactcac gcttaatcac ctcatcatct gctgacggta
     4741 aattattaga gatcccattt aacccggtgt atcggaattt tgacaaaggt cttcaggaaa
     4801 tcaccggctg gatcgaaaaa cgcttgtgtt aaaaatttgc taaaatttgc caatttggta
     4861 aaacagttgc atcacaacag gagattgcaa tgacgttacc gagtggacac ccgaagagca
     4921 gattgatcaa aaaatttacc gcactaggcc cgtatattcg tgaaggtaag tgcgaagata
     4981 atcgattctt tttcgattgt ctggctgtat gcgtcaacgt gaaaccggca ccggaagtgc
     5041 gtgagttctg gggctggtgg atggagcttg aagcgcagga atcccgtttt acctacagtt
     5101 accagtttgg tctgttcgat aaagcaggcg actggaagag tgttccggta aaagacactg
     5161 aagtggttga acgactggag cacaccctac gtgagtttca cgagaagctg cgtgaactgc
     5221 tgacgacgct gaatctgaag ctggaaccgg cggatgattt tcgtgatgaa ccggtgaagt
     5281 taacggcgtg agtgaaatgt gccggatgta tcacatccgg caatatccat taaaactgat
     5341 aggtcatgcc aaccgcgaca atatcatcat tattaatatt caatttgtta tcgctatcca
     5401 gttggttgat tttataatca acaaacgctg acatattttt gttgaaataa tacgtagcac
     5461 cgacgtcgat ataattgacc agatcttcat caccgatacc ttcaatatct ttccctttcg
     5521 ataagacata acccagcgat ggacgcagac caaagtcaaa ctggtattga gcgaccgctt
     5581 caaagttctg tgtcttattg gcaaagccgc cagttattgg cgtcattttg cgtgtttcag
     5641 aatagaaagt tgccagataa atattattgg catcgtattt cagacctgtt gcccatgctt
     5701 ctgcacgctt gcctgtgcca cggctttgca ggttctgctc gttggtgcga tctgagttgg
     5761 tataggcccc actaatggcg aaatcgctgc cgccaaagtc atatgtcaat gacgtgccga
     5821 agccatcgcc gttttgcttt ttaacgtcgc ggttttcgtt tttcccttga tattgcaggg
     5881 ttaagttcag gccatcgata acgccgaaga agtcggtgtt ccgatacgtc gccagaccgc
     5941 tggcgcgttt ggtcataaag ttgtcggtct gcgccgagga atcgccacca aattccggga
     6001 acatatcggt ccaggcttcc acgtcataca acgcgcccag gttacgacca taatcgaaag
     6061 aacccaaatc tttatatttc aacccggcaa aagcgagacg cgttttctgc tgtgcagtat
     6121 cactctccgc tttatttccg gcaaactccg cttcccaccg accataacca gtcagttgat
     6181 cgttaatttg tgtttcacct ttaaaaccaa aacggatata actctggtcg ccatctttac
     6241 tggcgttatc actcatataa tgcatagctt taactttgcc atagacatcc agtttattac
     6301 cgtctttatt atatatttct gcagcctgta cagatgcaga tgccacaatg cccatcacca
     6361 ctaatgccag agtgctcttt ttcattttca ttcctgattt taattaacgc gcgaatattc
     6421 agcgggagag ccccgttgaa aacaggaaag tttttaacct gagattgtta aagatatatt
     6481 acagattaat aatattctta aaatgtggta atttattaaa tctgtaataa aagcgtaaac
     6541 aactgccgct aggcttgctg atcccgcgca acaaaacgcc atgctttgct cgcagatggt
     6601 tggcaaccga cgacagtcct gctaaaacgt tcgtttgata tcatttttcc taaaattgaa
     6661 tggcagagaa tcatgagtga cagccagacg ctggtggtaa aactcggcac cagtg
//
LOCUS       DADMKF010000448         3781 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella sonnei strain CenAsiaIII
            SAMN12179898-rid11405823.guided.258, whole genome shotgun sequence.
ACCESSION   DADMKF010000448 DADMKF010000000
VERSION     DADMKF010000448.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12179898
            Sequence Read Archive: SRR9639883
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000940682.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000940682.1
            Genome Coverage       :: 106x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/25/2021 20:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,505
            CDSs (total)                      :: 4,416
            Genes (coding)                    :: 4,176
            CDSs (with protein)               :: 4,176
            Genes (RNA)                       :: 89
            rRNAs                             :: 2, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 116 of 240
            Pseudo Genes (incomplete)         :: 50 of 240
            Pseudo Genes (internal stop)      :: 92 of 240
            Pseudo Genes (multiple problems)  :: 18 of 240
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3781
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12179898-rid11405823.guided.258"
                     /strain="CenAsiaIII"
                     /serotype="sonnei"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Viet Nam"
                     /collection_date="Nov-2011"
                     /collected_by="University of Melbourne, Microbiological
                     Diagnostic Unit Public Health Laboratory"
                     /note="genotype: Global 3"
     gene            64..306
                     /locus_tag="JNC24_004501"
     CDS             64..306
                     /locus_tag="JNC24_004501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase"
                     /protein_id="HAY9161041.1"
                     /translation="MKTAALDLARERQAHEAGARTRATAHERTPQQERQKAAREAERG
                     REAWTLGQGMKKPVAGCYGRLTRWKGGGDVVYMALL"
     gene            complement(338..988)
                     /gene="tetR(A)"
                     /locus_tag="JNC24_004502"
     CDS             complement(338..988)
                     /gene="tetR(A)"
                     /locus_tag="JNC24_004502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(A)"
                     /protein_id="HAY9161042.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWH
                     FRNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAG
                     TRPGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGG
                     TVEQAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     gene            1094..2293
                     /gene="tet(A)"
                     /locus_tag="JNC24_004503"
     CDS             1094..2293
                     /gene="tet(A)"
                     /locus_tag="JNC24_004503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(A)"
                     /protein_id="HAY9161043.1"
                     /translation="MKPNRPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAH
                     YGILLALYALMQFACAPVLGALSDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYIG
                     RIVAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPH
                     APFFAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAV
                     FFIMQLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGE
                     RRALMLGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQG
                     QLQGSLAALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSG
                     AGQRADR"
     gene            complement(2325..3209)
                     /locus_tag="JNC24_004504"
     CDS             complement(2325..3209)
                     /locus_tag="JNC24_004504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HAY9161044.1"
                     /translation="MSLRTPDLLFTAIAPAIWGSTYIVTTQYLPNFSPMTVAMLRALP
                     AGLLLVMIVRQIPTGIWWMRIFILGALNISLFWSLLFISVYRLPGGVAATVGAVQPLM
                     VVFISAALLGSPIRLMAVLGAICGTAGVALLVLTPNAALDPVGVAAGLAGAVSMAFGT
                     VLTRKWQPPVPLLTFTAWQLAAGGLLLVPVALVFDPPIPMPTGTNVLGLAWLGLIGAG
                     LTYFLWFRGISRLEPTVVSLLGFLSPGTAVLLGWLFLDQTLSALQIIGVLLVIGSIWL
                     GQRSNRTPRARIACRKSP"
     gene            complement(3347..3739)
                     /locus_tag="JNC24_004505"
     CDS             complement(3347..3739)
                     /locus_tag="JNC24_004505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAY9161045.1"
                     /translation="MLFPNFSLGEEYEHAPPATNRQISPYLPSGRFRTGLPVEGLAIE
                     RGDLFYACPRASVFYGTALDADLRTRGVSTLVMAGISTTGVVLSSVAWASDADYDVRL
                     VQDCCYDPDRDAHEALLRSGFGGRVQVV"
BASE COUNT          718 a         1190 c         1169 g          704 t
ORIGIN      
        1 ccttagcgta cgattttttc cgaattctgc gggctcccct atctcatctg cgcaaggcag
       61 aacgtgaaga cggccgccct ggacctcgcc cgcgagcgcc aggcgcacga ggccggcgcg
      121 cggacccgcg ccacggccca cgagcggacg ccgcagcagg agcgccagaa ggccgccaga
      181 gaggccgagc gcggccgtga ggcttggacg ctagggcagg gcatgaaaaa gcccgtagcg
      241 ggctgctacg ggcgtctgac gcggtggaaa gggggagggg atgttgtcta catggctctg
      301 ctgtagtgag tgggttgcgc tccggcagcg gtcctgatca atcgtcaccc tttctcggtc
      361 cttcaacgtt cctgacaacg agcctccttt tcgccaatcc atcgacaatc accgcgagtc
      421 cctgctcgaa cgctgcgtcc ggaccggctt cgtcgaaggc gtctatcgcg gcccgcaaca
      481 gcggcgagag cggagcctgt tcaacggtgc cgccgcgctc gccggcatcg ctgtcgccgg
      541 cctgctcctc aagcacggcc ccaacagtga agtagctgat tgtcatcagc gcattgacgg
      601 cgtccccggc cgaaaaaccc gcctcgcaga ggaagcgaag ctgcgcgtcg gccgtttcca
      661 tctgcggtgc gcccggtcgc gtgccggcat ggatgcgcgc gccatcgcgg taggcgagca
      721 gcgcctgcct gaagctgcgg gcattcccga tcagaaatga gcgccagtcg tcgtcggctc
      781 tcggcaccga atgcgtatga ttctccgcca gcatggcttc ggccagtgcg tcgagcagcg
      841 cccgcttgtt cctgaagtgc cagtaaagcg ccggctgctg aacccccaac cgttccgcca
      901 gtttgcgtgt cgtcagaccg tctacgccga cctcgttcaa caggtccagg gcggcacgga
      961 tcactgtatt cggctgcaac tttgtcatgc ttgacacttt atcactgata aacataatat
     1021 gtccaccaac ttatcagtga taaagaatcc gcgcgttcaa tcggaccagc ggaggctggt
     1081 ccggaggcca gacgtgaaac ccaacagacc cctgatcgta attctgagca ctgtcgcgct
     1141 cgacgctgtc ggcatcggcc tgattatgcc ggtgctgccg ggcctcctgc gcgatctggt
     1201 tcactcgaac gacgtcaccg cccactatgg cattctgctg gcgctgtatg cgttgatgca
     1261 atttgcctgc gcacctgtgc tgggcgcgct gtcggatcgt ttcgggcggc ggccggtctt
     1321 gctcgtctcg ctggccggcg ctgctgtcga ctacgccatc atggcgacgg cgcctttcct
     1381 ttgggttctc tatatcgggc ggatcgtggc cggcatcacc ggggcgactg gggcggtagc
     1441 cggcgcttat attgccgata tcactgatgg cgatgagcgc gcgcggcact tcggcttcat
     1501 gagcgcctgt ttcgggttcg ggatggtcgc gggacctgtg ctcggtgggc tgatgggcgg
     1561 tttctccccc cacgctccgt tcttcgccgc ggcagccttg aacggcctca atttcctgac
     1621 gggctgtttc cttttgccgg agtcgcacaa aggcgaacgc cggccgttac gccgggaggc
     1681 tctcaacccg ctcgcttcgt tccggtgggc ccggggcatg accgtcgtcg ccgccctgat
     1741 ggcggtcttc ttcatcatgc aacttgtcgg acaggtgccg gccgcgcttt gggtcatttt
     1801 cggcgaggat cgctttcact gggacgcgac cacgatcggc atttcgcttg ccgcatttgg
     1861 cattctgcat tcactcgccc aggcaatgat caccggccct gtagccgccc ggctcggcga
     1921 aaggcgggca ctcatgctcg gaatgattgc cgacggcaca ggctacatcc tgcttgcctt
     1981 cgcgacacgg ggatggatgg cgttcccgat catggtcctg cttgcttcgg gtggcatcgg
     2041 aatgccggcg ctgcaagcaa tgttgtccag gcaggtggat gaggaacgtc aggggcagct
     2101 gcaaggctca ctggcggcgc tcaccagcct gacctcgatc gtcggacccc tcctcttcac
     2161 ggcgatctat gcggcttcta taacaacgtg gaacgggtgg gcatggattg caggcgctgc
     2221 cctctacttg ctctgcctgc cggcgctgcg tcgcgggctt tggagcggcg cagggcaacg
     2281 agccgatcgc tgatcgtgga aacgataggc ctatgccatg cgggtcaagg cgacttccgg
     2341 caagctatac gcgccctagg agtgcggttg gaacgttggc ccagccagat actcccgatc
     2401 acgagcagga cgccgatgat ttgaagcgca ctcagcgtct gatccaagaa caaccatcct
     2461 agcaacacgg cggtccccgg gctgagaaag cccagtaagg aaacaactgt aggttcgagt
     2521 cgcgagatcc cccggaacca aaggaagtag gttaaacccg ctccgatcag gccgagccac
     2581 gccaggccga gaacattggt tcctgtaggc atcgggattg gcggatcaaa cactaaagct
     2641 actggaacga gcagaagtcc tccggccgcc agttgccagg cggtaaaggt gagcagaggc
     2701 acgggaggtt gccacttgcg ggtcagcacg gttccgaacg ccatggaaac cgcccccgcc
     2761 aggcccgctg cgacgccgac aggatctagc gctgcgtttg gtgtcaacac caacagcgcc
     2821 acgcccgcag ttccgcaaat agcccccagg accgccatca atcgtatcgg gctacctagc
     2881 agagcggcag agatgaacac gaccatcagc ggctgcacag cgcctaccgt cgccgcgacc
     2941 ccgcccggca ggcggtagac cgaaataaac aacaagctcc agaatagcga aatattaagt
     3001 gcgccgagga tgaagatgcg catccaccag attcccgttg gaatctgtcg gacgatcatc
     3061 acgagcaata aacccgccgg caacgcccgc agcatcgcga ccgtcatcgg tgagaagttc
     3121 ggcaggtatt gggtggtgac aatgtaggtg ctgccccaaa tggcaggtgc tatcgctgtg
     3181 aacaataaat cgggcgtgcg taatgacatg cgattacaag acctccgcta cggcgatggg
     3241 tttttgagag ttgcaggcgc cgcgatgacc gcaccctgac ttggactggc cctgcctcgc
     3301 accaatccac ctgcggctat ggccgcgatc atgcgaggcc cgaaaattac acgacctgta
     3361 cacgtccgcc gaacccggaa cgcaacagag cttcgtgggc atcccgatcc gggtcgtagc
     3421 agcagtcctg gaccaaacgc acgtcgtagt ccgcatcact agcccaggcg acgcttgaaa
     3481 gaacaacgcc ggtggtgctt atcccggcca tgacaagcgt gcttacgccg cgcgtccgaa
     3541 ggtcggcgtc gagcgccgtg ccataaaaga cgctggctcg cggacatgca tagaaaaggt
     3601 cgccccgttc gatcgcaagc ccttcgacgg gcagaccggt gcgaaatcgt ccgctcggga
     3661 gatacggtga gatttggcga ttcgtcgccg gcggcgcatg ttcatactct tcgccgagcg
     3721 aaaagttggg gaacagcacg ggccggccgc gcaaggcgtg ctggcagccg tgcagaccct
     3781 g
//
